LOCUS       DAZGOB010000001        98468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGOB010000001 DAZGOB010000000
VERSION     DAZGOB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 98468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.001"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHLIF_000001"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHLIF_000001"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO9604082.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /gene="mepK"
                     /locus_tag="ACHLIF_000002"
     CDS             complement(804..1352)
                     /gene="mepK"
                     /locus_tag="ACHLIF_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO9604083.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHLIF_000003"
     CDS             complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHLIF_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9604084.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHLIF_000004"
     CDS             complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHLIF_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO9604085.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLANYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQITEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHLIF_000005"
     CDS             complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHLIF_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO9604086.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHLIF_000006"
     CDS             complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHLIF_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO9604087.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHLIF_000007"
     CDS             complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHLIF_000007"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO9604088.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11026..11805
                     /gene="elyC"
                     /locus_tag="ACHLIF_000008"
     CDS             11026..11805
                     /gene="elyC"
                     /locus_tag="ACHLIF_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO9604089.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGDGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11782..12675)
                     /gene="ycbJ"
                     /locus_tag="ACHLIF_000009"
     CDS             complement(11782..12675)
                     /gene="ycbJ"
                     /locus_tag="ACHLIF_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO9604090.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHLIF_000010"
     CDS             complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHLIF_000010"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO9604091.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHLIF_000011"
     CDS             complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHLIF_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO9604092.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHLIF_000012"
     CDS             complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHLIF_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO9604093.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHLIF_000013"
     CDS             complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHLIF_000013"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO9604094.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHLIF_000014"
     CDS             complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHLIF_000014"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO9604095.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17843..20107)
                     /gene="ycaI"
                     /locus_tag="ACHLIF_000015"
     CDS             complement(17843..20107)
                     /gene="ycaI"
                     /locus_tag="ACHLIF_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO9604096.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHLIF_000016"
     CDS             complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHLIF_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO9604097.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHLIF_000017"
     CDS             complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHLIF_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO9604098.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHLIF_000018"
     CDS             complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHLIF_000018"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO9604099.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHLIF_000019"
     CDS             complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHLIF_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO9604100.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHLIF_000020"
     CDS             complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHLIF_000020"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO9604101.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHLIF_000021"
     CDS             complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHLIF_000021"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO9604102.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26972..27664)
                     /gene="ycaP"
                     /locus_tag="ACHLIF_000022"
     CDS             complement(26972..27664)
                     /gene="ycaP"
                     /locus_tag="ACHLIF_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO9604103.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHLIF_000023"
     CDS             27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHLIF_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO9604104.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            29960..30817
                     /gene="focA"
                     /locus_tag="ACHLIF_000024"
     CDS             29960..30817
                     /gene="focA"
                     /locus_tag="ACHLIF_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO9604105.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            30872..33154
                     /gene="pflB"
                     /locus_tag="ACHLIF_000025"
     CDS             30872..33154
                     /gene="pflB"
                     /locus_tag="ACHLIF_000025"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO9604106.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            33346..34086
                     /gene="pflA"
                     /locus_tag="ACHLIF_000026"
     CDS             33346..34086
                     /gene="pflA"
                     /locus_tag="ACHLIF_000026"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO9604107.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34168..34758)
                     /gene="ycaK"
                     /locus_tag="ACHLIF_000027"
     CDS             complement(34168..34758)
                     /gene="ycaK"
                     /locus_tag="ACHLIF_000027"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO9604108.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            34858..35766
                     /gene="ycaN"
                     /locus_tag="ACHLIF_000028"
     CDS             34858..35766
                     /gene="ycaN"
                     /locus_tag="ACHLIF_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9604109.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(35767..37197)
                     /gene="ycaM"
                     /locus_tag="ACHLIF_000029"
     CDS             complement(35767..37197)
                     /gene="ycaM"
                     /locus_tag="ACHLIF_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO9604110.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPFLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTASAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(37406..38554)
                     /gene="ycaD"
                     /locus_tag="ACHLIF_000030"
     CDS             complement(37406..38554)
                     /gene="ycaD"
                     /locus_tag="ACHLIF_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9604111.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            38869..39495
                     /gene="ycaC"
                     /locus_tag="ACHLIF_000031"
     CDS             38869..39495
                     /gene="ycaC"
                     /locus_tag="ACHLIF_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO9604112.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(39531..40394)
                     /gene="dmsC"
                     /locus_tag="ACHLIF_000032"
     CDS             complement(39531..40394)
                     /gene="dmsC"
                     /locus_tag="ACHLIF_000032"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO9604113.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(40396..41013)
                     /gene="dmsB"
                     /locus_tag="ACHLIF_000033"
     CDS             complement(40396..41013)
                     /gene="dmsB"
                     /locus_tag="ACHLIF_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO9604114.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(41024..43468)
                     /gene="dmsA"
                     /locus_tag="ACHLIF_000034"
     CDS             complement(41024..43468)
                     /gene="dmsA"
                     /locus_tag="ACHLIF_000034"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO9604115.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(43707..44999)
                     /gene="serS"
                     /locus_tag="ACHLIF_000035"
     CDS             complement(43707..44999)
                     /gene="serS"
                     /locus_tag="ACHLIF_000035"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO9604116.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(45090..46433)
                     /gene="rarA"
                     /locus_tag="ACHLIF_000036"
                     /gene_synonym="mgsA"
     CDS             complement(45090..46433)
                     /gene="rarA"
                     /locus_tag="ACHLIF_000036"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO9604117.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            complement(46444..47055)
                     /gene="lolA"
                     /locus_tag="ACHLIF_000037"
     CDS             complement(46444..47055)
                     /gene="lolA"
                     /locus_tag="ACHLIF_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO9604118.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            complement(47210..51316)
                     /gene="ftsK"
                     /locus_tag="ACHLIF_000038"
     CDS             complement(47210..51316)
                     /gene="ftsK"
                     /locus_tag="ACHLIF_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO9604119.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQSYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            complement(51451..51945)
                     /gene="lrp"
                     /locus_tag="ACHLIF_000039"
     CDS             complement(51451..51945)
                     /gene="lrp"
                     /locus_tag="ACHLIF_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO9604120.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            52489..53454
                     /gene="trxB"
                     /locus_tag="ACHLIF_000040"
     CDS             52489..53454
                     /gene="trxB"
                     /locus_tag="ACHLIF_000040"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO9604121.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGSGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53577..55343
                     /gene="cydD"
                     /locus_tag="ACHLIF_000041"
     CDS             53577..55343
                     /gene="cydD"
                     /locus_tag="ACHLIF_000041"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO9604122.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            55344..57065
                     /gene="cydC"
                     /locus_tag="ACHLIF_000042"
     CDS             55344..57065
                     /gene="cydC"
                     /locus_tag="ACHLIF_000042"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO9604123.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFRLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            57107..57811
                     /gene="aat"
                     /locus_tag="ACHLIF_000043"
     CDS             57107..57811
                     /gene="aat"
                     /locus_tag="ACHLIF_000043"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO9604124.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            58096..58314
                     /gene="infA"
                     /locus_tag="ACHLIF_000044"
     CDS             58096..58314
                     /gene="infA"
                     /locus_tag="ACHLIF_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO9604125.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            58568..58655
                     /locus_tag="ACHLIF_000045"
     tRNA            58568..58655
                     /locus_tag="ACHLIF_000045"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58602..58604,aa:Ser,seq:gga)
     gene            complement(58998..61274)
                     /gene="clpA"
                     /locus_tag="ACHLIF_000046"
     CDS             complement(58998..61274)
                     /gene="clpA"
                     /locus_tag="ACHLIF_000046"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO9604126.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(61305..61625)
                     /gene="clpS"
                     /locus_tag="ACHLIF_000047"
     CDS             complement(61305..61625)
                     /gene="clpS"
                     /locus_tag="ACHLIF_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO9604127.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            complement(61724..61798)
                     /gene="yljB"
                     /locus_tag="ACHLIF_000048"
     CDS             complement(61724..61798)
                     /gene="yljB"
                     /locus_tag="ACHLIF_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO9604128.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            61948..62172
                     /gene="cspD"
                     /locus_tag="ACHLIF_000049"
     CDS             61948..62172
                     /gene="cspD"
                     /locus_tag="ACHLIF_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO9604129.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            complement(62245..64191)
                     /gene="macB"
                     /locus_tag="ACHLIF_000050"
     CDS             complement(62245..64191)
                     /gene="macB"
                     /locus_tag="ACHLIF_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO9604130.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(64188..65303)
                     /gene="macA"
                     /locus_tag="ACHLIF_000051"
     CDS             complement(64188..65303)
                     /gene="macA"
                     /locus_tag="ACHLIF_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO9604131.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            65454..66410
                     /gene="ybjX"
                     /locus_tag="ACHLIF_000052"
     CDS             65454..66410
                     /gene="ybjX"
                     /locus_tag="ACHLIF_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO9604132.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            complement(66407..68065)
                     /gene="ybjD"
                     /locus_tag="ACHLIF_000053"
     CDS             complement(66407..68065)
                     /gene="ybjD"
                     /locus_tag="ACHLIF_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO9604133.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HSSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            68491..69186
                     /gene="aqpZ"
                     /locus_tag="ACHLIF_000054"
     CDS             68491..69186
                     /gene="aqpZ"
                     /locus_tag="ACHLIF_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO9604134.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            69681..70580
                     /gene="lysO"
                     /locus_tag="ACHLIF_000055"
     CDS             69681..70580
                     /gene="lysO"
                     /locus_tag="ACHLIF_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO9604135.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTSLAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            70724..72376
                     /gene="hcp"
                     /locus_tag="ACHLIF_000056"
                     /gene_synonym="priS"
     CDS             70724..72376
                     /gene="hcp"
                     /locus_tag="ACHLIF_000056"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO9604136.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            72388..73356
                     /gene="hcr"
                     /locus_tag="ACHLIF_000057"
     CDS             72388..73356
                     /gene="hcr"
                     /locus_tag="ACHLIF_000057"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO9604137.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            73489..75207
                     /gene="poxB"
                     /locus_tag="ACHLIF_000058"
     CDS             73489..75207
                     /gene="poxB"
                     /locus_tag="ACHLIF_000058"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO9604138.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            75244..76245
                     /gene="ltaE"
                     /locus_tag="ACHLIF_000059"
     CDS             75244..76245
                     /gene="ltaE"
                     /locus_tag="ACHLIF_000059"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO9604139.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            76256..77686
                     /gene="ybjT"
                     /locus_tag="ACHLIF_000060"
     CDS             76256..77686
                     /gene="ybjT"
                     /locus_tag="ACHLIF_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO9604140.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYSTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            77785..78798
                     /gene="ybjS"
                     /locus_tag="ACHLIF_000061"
     CDS             77785..78798
                     /gene="ybjS"
                     /locus_tag="ACHLIF_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO9604141.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWTV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(78795..79625)
                     /gene="amiD"
                     /locus_tag="ACHLIF_000062"
     CDS             complement(78795..79625)
                     /gene="amiD"
                     /locus_tag="ACHLIF_000062"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO9604142.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(79622..79945)
                     /gene="ybjQ"
                     /locus_tag="ACHLIF_000063"
     CDS             complement(79622..79945)
                     /gene="ybjQ"
                     /locus_tag="ACHLIF_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO9604143.1"
                     /translation="MQFSTTPTLEGQIIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            80071..80586
                     /gene="ybjP"
                     /locus_tag="ACHLIF_000064"
     CDS             80071..80586
                     /gene="ybjP"
                     /locus_tag="ACHLIF_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9604144.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            80804..81532
                     /gene="artP"
                     /locus_tag="ACHLIF_000065"
     CDS             80804..81532
                     /gene="artP"
                     /locus_tag="ACHLIF_000065"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO9604145.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            81550..82281
                     /gene="artI"
                     /locus_tag="ACHLIF_000066"
     CDS             81550..82281
                     /gene="artI"
                     /locus_tag="ACHLIF_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO9604146.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            82288..83004
                     /gene="artQ"
                     /locus_tag="ACHLIF_000067"
     CDS             82288..83004
                     /gene="artQ"
                     /locus_tag="ACHLIF_000067"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO9604147.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            83004..83672
                     /gene="artM"
                     /locus_tag="ACHLIF_000068"
     CDS             83004..83672
                     /gene="artM"
                     /locus_tag="ACHLIF_000068"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO9604148.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            83964..84695
                     /gene="artJ"
                     /locus_tag="ACHLIF_000069"
     CDS             83964..84695
                     /gene="artJ"
                     /locus_tag="ACHLIF_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO9604149.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(84870..85997)
                     /gene="rlmC"
                     /locus_tag="ACHLIF_000070"
     CDS             complement(84870..85997)
                     /gene="rlmC"
                     /locus_tag="ACHLIF_000070"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO9604150.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(86038..86526)
                     /gene="ybjO"
                     /locus_tag="ACHLIF_000071"
     CDS             complement(86038..86526)
                     /gene="ybjO"
                     /locus_tag="ACHLIF_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO9604151.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(86586..87431)
                     /gene="potI"
                     /locus_tag="ACHLIF_000072"
     CDS             complement(86586..87431)
                     /gene="potI"
                     /locus_tag="ACHLIF_000072"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO9604152.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(87428..88381)
                     /gene="potH"
                     /locus_tag="ACHLIF_000073"
     CDS             complement(87428..88381)
                     /gene="potH"
                     /locus_tag="ACHLIF_000073"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO9604153.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(88391..89524)
                     /gene="potG"
                     /locus_tag="ACHLIF_000074"
     CDS             complement(88391..89524)
                     /gene="potG"
                     /locus_tag="ACHLIF_000074"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO9604154.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(89619..90731)
                     /gene="potF"
                     /locus_tag="ACHLIF_000075"
     CDS             complement(89619..90731)
                     /gene="potF"
                     /locus_tag="ACHLIF_000075"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO9604155.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(91082..91558)
                     /gene="ybjN"
                     /locus_tag="ACHLIF_000076"
     CDS             complement(91082..91558)
                     /gene="ybjN"
                     /locus_tag="ACHLIF_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO9604156.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(91646..92548)
                     /gene="rimK"
                     /locus_tag="ACHLIF_000077"
     CDS             complement(91646..92548)
                     /gene="rimK"
                     /locus_tag="ACHLIF_000077"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO9604157.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(92609..93331)
                     /gene="nfsA"
                     /locus_tag="ACHLIF_000078"
     CDS             complement(92609..93331)
                     /gene="nfsA"
                     /locus_tag="ACHLIF_000078"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO9604158.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(93315..93602)
                     /gene="ybjC"
                     /locus_tag="ACHLIF_000079"
     CDS             complement(93315..93602)
                     /gene="ybjC"
                     /locus_tag="ACHLIF_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO9604159.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            93762..94019
                     /gene="grxA"
                     /locus_tag="ACHLIF_000080"
     CDS             93762..94019
                     /gene="grxA"
                     /locus_tag="ACHLIF_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO9604160.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(94049..94426)
                     /gene="ybjM"
                     /locus_tag="ACHLIF_000081"
     CDS             complement(94049..94426)
                     /gene="ybjM"
                     /locus_tag="ACHLIF_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO9604161.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            94696..96381
                     /gene="ybjL"
                     /locus_tag="ACHLIF_000082"
     CDS             94696..96381
                     /gene="ybjL"
                     /locus_tag="ACHLIF_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO9604162.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(96556..97092)
                     /gene="rcdA"
                     /locus_tag="ACHLIF_000083"
     CDS             complement(96556..97092)
                     /gene="rcdA"
                     /locus_tag="ACHLIF_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO9604163.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            97176..98384
                     /gene="ybjJ"
                     /locus_tag="ACHLIF_000084"
     CDS             97176..98384
                     /gene="ybjJ"
                     /locus_tag="ACHLIF_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9604164.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            98384..>98468
                     /locus_tag="ACHLIF_000085"
     CDS             98384..>98468
                     /locus_tag="ACHLIF_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFO9604165.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMA"
BASE COUNT        23770 a        26211 c        24354 g        24133 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgtc acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag cagacgtcaa
     1441 gatgactcaa ccccagcagt gacgggggct gcagagaatc gcccccaatc atgctaatta
     1501 ttacgacaac tgatttcccc gaactacttc atttacctga ttaattgttc cgctttcgat
     1561 acgatttgcg agctggatcg cgcaggcaga tcataattgt aaatatctgt acgatactgg
     1621 gtacgaccat ctgcaccaac aaaggccgtc aggtagtaga gattcaccgg aatcgactgc
     1681 cgaatattga cgtaacgtgt atcaccctgc ttcagcgcat cagaaatacg tttgtcattc
     1741 cagcctgcat cctgcaacag catattcgcc agatcggaag ctttattcac tcgtacacag
     1801 cctgagctca atgcgcgtgt atcacgcttg aacagattgt ggttcggcgt gtcatgcaaa
     1861 taaatggcct ctgaactcgg catattgaat ttatagcgcc ccagcgagtt ccgtgggcct
     1921 ggagcctgct ggaagcggaa cggtaaattc gaggccgtga ttgtagacca gtcaacctgc
     1981 catgggtcaa tcgcttctct gctgttccag ccgcgcatca ccgtatagcc atggctttcg
     2041 agatatcccg gatcgttgcg cacttttggc agaatatctt tgcgtgccag agttggaggt
     2101 acgttccacg gcgggtttac cactacgttg ttaagggcac tgctcatcat cggcgttttg
     2161 cgatcggggc gaccgacaat gactcgcgaa tccagcacct gattgccgtt ctgatagtag
     2221 accagcgaat aggccggaat gttaaccatg atcccggtag aaagctctgt tggcagcaag
     2281 cgcaatcgct ggatgttgag agccaacacg ccagcacgct gggcgggcgt tacgtttaac
     2341 cagtcacgtg ttgccgggcc aatagcacca tctgccccca atccttgcca tgcctgaaaa
     2401 cgtttaacgg cttccaccag ttcattatcg taggcggcac gaacggcagg cgcaggttta
     2461 ctacgagacg tcgtttgctt gtccatcggc ttagtttctg ctgtttcaac agtaacagcg
     2521 gatgggctga ctaccgcgtc agttggcgtg tcatcgccag gtagagtaat tttcggcccc
     2581 ccgtccaaca tgcctgtgcg ttgcaatatt tcgcgcaacg ccggtacgtc gttactccac
     2641 tgccctgggc gcaacgttgc tttgccggtc agttggggcc acggtttggt gtcactgagt
     2701 aaggccagta acgattcatg catcgccgca tattgcggat gctgcggtgc cagtcctgca
     2761 acaaacgtag gcaattgacc tttatccagc gccagctgcc attggttaat caccgagagc
     2821 ggcggcgttg caagtgcata aggtttactg ctatatagcc agcgagtgcc tttgaccgga
     2881 atatttgcaa tgaaatggag atagcccatc atcgcatcag agagcactac gtcgcgtgcc
     2941 atcccgttaa caccaggatc ggtcagtaac tctacccatt tattaaactg cggctggaaa
     3001 ccggcaatcg ccacctctgc cagctgttgc tggaaggctt taacagcatc acggttttcc
     3061 cacatgggtt gcatatcgcg tgcggcatac aacagttgaa gctggttaag ataaaccggc
     3121 ttgtaacctg cgggtaattg agattcgatt tgcgtgcggg ctttttctgc cgcaccttca
     3181 ggcaatggct ggatccccgc cattattgcc gtggcttgcg cctgcggcag tgcctcgccc
     3241 tgttcactga cagccaccgg gctgtcgcca gggattactt caggctcatc ggcctgcgca
     3301 ttgaacagtg gagcgaatgt tacggccagg cacaaactga ttgccgacag ccgacgacca
     3361 cacatcatat taagcaacat cccttgcccc ctgtttttat catactctga atagccaggc
     3421 tgttatttta cgacttgcct gtacaacgcc aatactcacg aaagtatgac gagcaagcct
     3481 gttactcatc aggcttatct tcagtatata aacgccgata cgtttttaca taacctaatg
     3541 taaagaagtt taaagaaaag cgcgccgtaa tgtgccactt ttttagtgcg gaaacggagt
     3601 tttcggaaaa agaaaaggcg gcattgctgc cgccttaatt ttaactcgcc tgagaaggcg
     3661 cttcgtcact tcctggaagc gtttcaggga gttgcggcgc aaatcctcgc aggccgacga
     3721 cgtgaacgtg ttcggtattc tggaagactt tacgcaccag tttataggtg gtgcctttct
     3781 ccgggctgat attttccggc gctgcgatga tgagctgcat ttgcaaacgc tcacacaatt
     3841 caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc
     3901 ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca
     3961 ccatcaccag aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact
     4021 ctgcgcgcaa ccaaccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc
     4081 ggtaatccag cagttcttcg ccaatggtct gcggcgtgcg ctgtcccata tcaatctgcg
     4141 gattaagacg ctgatacaat ttcgccaacg cttccgagaa ggtcagacgg ttgctgttaa
     4201 acagatcctg atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt
     4261 cacgcacgtt cacgttgagg cgcacgctgt tcacctgacc aaacgatacg ttctgcaacc
     4321 cctggttgag catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg
     4381 ccacgctgcg ggaactgatc gccagtttct gttcacggga ggttaattct tcggtcagac
     4441 ggctaagttc gatctccatc tgttcgatag cttccaccgg atcatcggta cgaataatat
     4501 cctgacgaat acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgct
     4561 ccggacgttt cggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca
     4621 ccgccagacg cagcgcaccc aatgccttat ccgacataga gcgcaaatca tcagcggaga
     4681 gataagccag ctcacgacgg tgtaagcggc gctcaacgcc gttatctttc accatgcgca
     4741 tcaccgcaca ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc
     4801 gctccagctt gcgcagtttg cgggtcaggt tgtccatctc cgcttcgcag aaggtaagcg
     4861 ctttttccag ttgattgcgg cgtgaacggt tattgctcag ttgcgcatgc agctcgtcac
     4921 ggcgaatacg cgcccgctct tctgccccgc tatcagcacg cacgccgata tcctgcaatt
     4981 cacgttgcag atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca
     5041 gcacctggtt gtactgactc aactgcgctg cgtgaccacg caacgcttca cgagcgcgag
     5101 tacgctccgc ttccgcctgt tccagacgtt cacgcagctt ttcgttgaga tcgctattac
     5161 cgctcagcat ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg
     5221 tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt
     5281 cttttagctg ttcgaactgt tccgggtcgc tctgcaatac cgaaacgatc ggttccagtt
     5341 tcgccagttg attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac
     5401 gttcgcggat ttcatcgacg cgatccgcca ggctgtcatc agccaacagg ttgagacgcg
     5461 gcagaatgcg gttcagcgcc gtaacgcctt ctttcgcctg ctcaaactga atacgctgct
     5521 gctggttatc attttcatga ttactcaacg cccgctccag ttcgacgcga cggctgttca
     5581 gttgacggat ttctgcttcc gggtcagact caaacgcaac cgccagatga ctgccgataa
     5641 agcggctgaa cgcctgatgc agacgctgag ttttctgtac atcaaaggag agcgtggcga
     5701 agcgttcgga aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag
     5761 cacgaccaaa cagcggcact tccgggaaac gtgaataacg ccactgacga tcggcgattt
     5821 tcaccactac cgctttttcc agctcatcaa cgctgaacac gctgtcatcg aatgactgcg
     5881 gatctccttc gatcagatag agatcttccg ggcaatcggt caagccttcc aggtgttcag
     5941 ttatctgtga cagatctggc accacgatgg cgtggcgtga cgggccatac agcgctgaga
     6001 agtacggcgc atcttccagg ctaacgtcgt cataaatttc tgacagcagc acaccaccaa
     6061 aacgctccgc cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt
     6121 cgatctcttc atcgacggcg tttttgcgcg cgcccacttc atcgcgttca acaatcgcct
     6181 ctcgctcacg ctccagcaac tgttgcagat attcggtgac gtcctggctg gaggtaaact
     6241 cttcgccgca ctgttcgctc aactggttga gactgttttg cgctgccagc caaaccggcg
     6301 cacgctgcat caaactctga atgcgagact gcagctgttc ctgttcctgg cgcagtgcca
     6361 tgcgctcttc acgggcgtta gacacgctat cggaaagaga ggcaatgcgc gcttccagct
     6421 cctgatgcag agcttccagc tcgtcaatgt caaaattctt gccctgacgt ttgcagaaat
     6481 ctgccagcag acgctcagct tcttgctgtt cgcgcagacg ctgttccagt tcgcttaagc
     6541 gcatccgcaa cggctgaacc tgctctgcca ggtgacgctg atcgacccct tcgcgcaata
     6601 gttcgcgagc gacatcccac gcctcgttac gcgccagcgg gccgttgatt gccaccacca
     6661 gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa
     6721 gagagagcat tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg
     6781 cggcgctgtc ggcggttaag tccggcagat ggcacagttc tttggcgcga ttaagcgcag
     6841 caatcgcctg gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt
     6901 tagcaagctg gcttttcagc tcgtccactt ccagttcggc agcttccgcg cgagcctcgt
     6961 tctcttcctg gcgttcgatg gcttctgcca ccacttcgtt ttgctcttcc agacggatct
     7021 gcagctcatc gagatccgct tcgtagcgtt cgatcttctc ctgctgacgc agtgcggttt
     7081 gcaccaggtt caggtgatcg ctggccgcct gataatccgc ttccagatcg ccttcggcac
     7141 cgttgtgctc tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt
     7201 gctgacgcga agtatgtaac tcgcgacgaa actccagggc tttgtcgaga tggacacggc
     7261 gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt ggtagcttcg ctgatcagat
     7321 gcttaaacag gtcacggtcc gactgggtga cacgaatcgc ttccagcgtc atgcggtttt
     7381 cacgcagcgc cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca
     7441 ggtagtcgcg cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga
     7501 tcagacgata gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca
     7561 gatcgaacat cagcgagtgg taatcggtaa tggagttgaa ctgtttaaac tgcacgcctt
     7621 ccatcgcctc gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt
     7681 tcagggtttc ggtcaccagc tgtgtcggct gcaccgacat cggcagtccc tgaatggcaa
     7741 atggtttgat atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca
     7801 ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca
     7861 gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac
     7921 ggaaatgcag cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atggtggtgg
     7981 atttacccgc cccgttaccg ccagaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc
     8041 gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca
     8101 ttattcttcc tctccgctat ctggctgatt ttcttcggtt tcatcgttga gttgcagatg
     8161 attttcaatc ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc
     8221 gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc gaaacttgct
     8281 gctgtcgtgg cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg
     8341 tactttctcc tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag
     8401 tttcagcagt tttgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt
     8461 gaaaatcccc tcattcgcca gccgttccgg gctgagatag agataacaga ggattttccc
     8521 gaccatcata tccagttccg acaagacgga acgagggatc agcgtggtgg aacgtgggcg
     8581 taaatagaag aacccttctg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc
     8641 ttccagatat tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat
     8701 atggcggcct gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg
     8761 cgccagctta accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg
     8821 ctccgtaatc attaatcggc tgccatttcg ctggcagtcc ggtgaaatct gcttgcgcta
     8881 cgccaagacg taccgcctga tcaataacaa tacgcgcaac gtcaaagtga cgtgcacgcg
     8941 gatactgtga cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt
     9001 tgtacacggc aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact
     9061 cttcgtattc cagatcctca ggaagttccc cagtcacttc ttcatcgcgc agtgccatct
     9121 cttcgtcacg catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat
     9181 caaaataggt ttgtaccgac tgacgtaacc gctgagcaaa gacgcggttt ttatccatat
     9241 cgatcgcggt acgaataaat ttgtgtacgt ggcggtcgta gccaatccac aagtcgatgg
     9301 attgctggcc ccaactgata atacgatcga gtttgctctg cagatcgaac accagacgat
     9361 cgacgaaatg cagatcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct
     9421 gcaatttgtc gcctgccgct tccagcgtat cctgcaattc acgcagcgtc ccggaagttt
     9481 ccgaaagcaa caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg
     9541 cgatatcgtc cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac
     9601 tgtcgaaaat ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa
     9661 attcatcacc gccctcttcg gcggcatctg ctgcgcgttt gagctcaccc gccacaatcg
     9721 acaactgcat agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa
     9781 tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa
     9841 agcggttcag cagacgttga cgcaccatgt cgttgatcgc gttattggcg cgcacgccga
     9901 tggtttcgct ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc
     9961 cttcactcat ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag
    10021 agagtcggtc taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt
    10081 cggggactgt ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt
    10141 gacatgaata taacgcccca gggtaatata cggttcctga cggcaataac gcgtttctaa
    10201 ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc tcgcgcagat aatcatgaaa
    10261 cacgcgaacg cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac
    10321 ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc
    10381 cgcctgcacg taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt
    10441 gtagaacatt aacgacaaca cgccgcctgg acgcaacact gaccacaggg tctgcaatac
    10501 actgcgggga tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt
    10561 ttccaaatgc gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac
    10621 accttttgct tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa
    10681 aatgacctga tgcccacgct cggccatttt gattgcggtc tgcccttctc caccgccagc
    10741 atccagcaca cgcagttttt gcgggcccat ttccgccagc acgcgatcga gatcctgcca
    10801 cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc
    10861 cgcaatatca tcaaaattgc gatcctgcat tgctttctcc accggttggc taagacgttt
    10921 tctgataaag acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat
    10981 cgctggcgat cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg
    11041 aaaaaagtga ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct
    11101 ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt
    11161 gggtggctgg cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc
    11221 atcgaaagca cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc
    11281 ggagatggtt atacctggaa cccgcagtgg gcaccgagct ctaatttaat caataacagt
    11341 ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa atccgggatc aaaactgatc
    11401 ttcacgggag gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt
    11461 gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc
    11521 gaagaagaag ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc
    11581 tccgcatcac atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta
    11641 ccagccccgg cgaatcagtt ggccatcgac tcgccgctta acccgtggga acgggcaatc
    11701 ccttctccgg tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc
    11761 tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc
    11821 gaagttgcgc cgactaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac
    11881 atacaaccaa cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca
    11941 gagcaaatct tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc
    12001 ccaaagcatt agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag
    12061 catgctgcgt aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa
    12121 taatgccggg agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc
    12181 ggtgttattg aactgattga gcgtggtcca tagcacttca acatgttgca ggtaccatga
    12241 gggccagaaa ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg
    12301 gtgccaggcc agtaaggctt caacgatttg gtctttgagc tgttcccagc gttctggtgt
    12361 tcgggctggt gcctccaccg aaacaccacg catccgctcc agtagcagga catcagggcc
    12421 ggggtgttct tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct
    12481 tgccagcatg gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct
    12541 tgccattaac ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc
    12601 tttttcattg acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc
    12661 acgcagctgt tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa
    12721 tataaaagat gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta
    12781 acggtacgac actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc
    12841 agcgcgaacg cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc
    12901 aacagcaaca tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat
    12961 gtgttctaac ggacttgcct gccagttgac gtaacgacgg ataaagcctg cacggtagcc
    13021 ataaatacca agatgacgca ggaagttatc gccaacggtt tcaaggcctt ctgcaaaacg
    13081 atcacgatcc caaggaatgg tggcgcgtga gaagtacagt gcataccctt cagcgtcgag
    13141 aaccactttc accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcaccgccag
    13201 agtcgccata cccacctgac gctgagcgag gttatcagca acctgacgaa tgattgtcgc
    13261 agggatcatc ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca
    13321 tttttcgaca acttccgcca gacgttctgt tcctgactga tgatcggcgc gcgtcataca
    13381 tacttcaccg ccagcggctt caacggcgcg ggcaacatcc tcatgatcgg ttgccacgat
    13441 gatgcgctcg gcacctgatt cacgcgcgcg ttcaagaaca tgaacaatca tgggtttgcc
    13501 gttaatatca accaatggtt taccgggcag acgcgtggac gcgtagcgcg cgggaataat
    13561 gaccacaaaa ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc
    13621 aacactggaa tgccatcgcg gagcgggaaa gccaggttat ccagtttgca aataagctct
    13681 tgtttttcct ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc
    13741 aaacgatgat ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata
    13801 atcatttatg cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc
    13861 gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc
    13921 tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag
    13981 ataacttcaa ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc
    14041 ccacgatgta ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag
    14101 cactccagcc ggtagctaaa atcaaaaagc tgctctgcgc gtttgcgatc ccagacaaca
    14161 ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc
    14221 tcgagtagcg gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca
    14281 gcaatgattt gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc
    14341 agccgatagt aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta
    14401 tccagcatga tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca
    14461 ggcatgacac ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc
    14521 acctttccgg cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag
    14581 ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac gtaccggccc cttatcatga
    14641 atatgctgaa ttaactgtgc aatttccgcc tcatgttcct gcatccaggc gtctttgtat
    14701 ttccagccca ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctacgc
    14761 ggcataaagc aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca
    14821 tccagccact gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg
    14881 gcaacaatat tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata
    14941 tcctccaacg acgcttgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaga
    15001 ttacgcgcat cagcaaggga gaggtgcggc agcgacattc atgactccat caatcgaacg
    15061 ctgccgcggc gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct
    15121 ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag
    15181 gcccggcatt tcaccgcatc tttttcagtc attaccagcg tttgcccggc gcttaccaac
    15241 gcactgacat ccgcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt
    15301 tgtacgccac acatcttcag cgtggcaaaa aagcgcggcg gatgcccaat ccccgccatc
    15361 gccactacat gttcaagctg agcaacgtca caacgcgtac cggtacgtaa attcaccgcc
    15421 tgacccggca gcagatgcat ggggatttca ccgctgcgag ggacaccgcc gttgacgatt
    15481 accgcatcaa ccgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac
    15541 cagccattgc caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga
    15601 cggtaatgct gtaaaccgtc gtcggttacg atgatctgca catcagggtg ttgcgccaga
    15661 atggctttta ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga
    15721 taaatcaaca caggttcatc acccgcctgt gctgttgtgg tatctgccga caataacagc
    15781 ggataagatt cagccttacc accatatccc cgcgatacga cccccacgcg aataccgcgc
    15841 tgttgcaact gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg
    15901 agattaccaa ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta
    15961 tagcaaagac ggatcgcgcc acttaccagg ccatacaacc aggagagtgg cagcaatagc
    16021 cgccacaaag gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt
    16081 gaagttgcgc gtaaacgccg cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa
    16141 tgacaccatc ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg
    16201 caatcaccag agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt
    16261 cggattcggt atccagagcc gaggtagctt catccagaat cagaatcggg ctatcacgca
    16321 gcaaggctcg agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt
    16381 caccaatcac tgtatcgaga ccgttatcca tcttattgat gaagtccatg gcgtaggcca
    16441 tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc agtccgtgcg taagcaatgt
    16501 tgttagcaac cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac
    16561 gtaacgacgc cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat
    16621 caatatcgta aaaacgagtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc
    16681 caaccagagc aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcaggta
    16741 cgtcacgtcc cggataagta aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct
    16801 cgatcacgcg cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct
    16861 gacaagccgc cataccgcgc tggaactggg cgttaacgtt ggtcagtgat ttcagcggac
    16921 gcatcagtgc aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggctatcca
    16981 tgacacttgg gaagctcgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct
    17041 gaatgatcgg atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc
    17101 ggttgctgac tttatcaaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt
    17161 ctttgtggcc cttcagcatt tgttcggcgc tggtggtcac ctgccccatg gtgttctgca
    17221 tgtttttact gatgttgcga aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg
    17281 gtgccagcac aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca
    17341 ggccgatgat tgacgcacct tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag
    17401 caacctgttc ggaatcgtag gtgatacgtg acaacagcgt ccccgttgac tgtttgtcaa
    17461 aaaatgaaac cggcattccc atcatgtgac caaacaggcg gcgacgcatg gtcattacca
    17521 cctttcctga tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa
    17581 tcatcagccc gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa
    17641 agccatcatc aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga
    17701 ggattaacgc tacgcccgcc acgatcagac ccgctttaaa aggcgcaatg gttggccaca
    17761 gtcggcggaa cgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca
    17821 tttgttgaaa tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat
    17881 accaacgagg taaaatttgc tctctgaggc tgctaatccg ccagccttgc gctgaaaaat
    17941 tgaccgtcac ttgaccctga tgtggagtat caagccattg atatccttgc tgttgatagc
    18001 gatgcttaac tttattagag ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg
    18061 ccacttttcc attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt
    18121 gaggtacctg aagcaatgtt gtctgcactt gctgccagta acggcttagc atcttttgtt
    18181 cagcggggac ttcaatatca ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa
    18241 ccacacagga atggttattt cctttatcgt tgctagcttg taaaggccag tgcacgctga
    18301 aacgcaatcc ttgccattgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt
    18361 ttaacggact tctgatccat aacatcggcc atgtgtgcaa tattgagtcc agccctcccc
    18421 ggtgatccag atgttcatga ctcagaataa cgccttccgg ttcaagatta tgccagtgga
    18481 gccaggggat aatcagttgt tgcccactat ccccttcggg ccaggccagt cctgtgtcat
    18541 agagaatcgc tttgccgttt ctggctatca ccattgccag cccttgcccg acatcaagca
    18601 tgtacacctg ccactcgtca ggtcgaggtt tttgccacag cggccagcac atcagcaagc
    18661 ctacagccac acacattgct ggcaacgttc gccaggcgtt taatcgccat accaccagta
    18721 agaaccatgg ggaaaatgat agccattgcc aacgttcagc aatgttgatc cacccttctg
    18781 gcaaactctt taacccccag aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc
    18841 cttgctccag gattaacggc ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa
    18901 ccgtgataaa tgtcaccaag ggaattgcta atagatttgc aataaacgag gtcagactaa
    18961 tgccatgaaa tatgacgatt tgcacaggca taagcaggag tgtgattccc agttgcaaat
    19021 gaatgaggga aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc
    19081 actggtacca aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga
    19141 gaatggcaac aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc
    19201 cactccactg tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac
    19261 gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccac
    19321 caattaatgg catttgccag tggatccagc gcccaggcag ataaacttgc ccaccgcgaa
    19381 tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat
    19441 gcgccgttcc agtatcacgc atgatatttt tgatttcttt ggggacggat aaccgttccc
    19501 ccatacctaa accaagaata accgcattcc acatataggg ttgcagcgtt gtttgtagtg
    19561 acgccagata ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaagc
    19621 ggccggtgag cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat
    19681 taagttggcc atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa
    19741 ccgcttgcgg aagatattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac
    19801 cttgtagatg agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa
    19861 caattgcatc ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg
    19921 ataatatgcc ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag
    19981 ggataaatgc cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcccaggca
    20041 attggggcaa aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta
    20101 ttttcatgca cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt
    20161 gggaattttg cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct
    20221 caaaactctg cgaggcagct tccagttcct gattagagaa atgaaaaaag cacccgacag
    20281 gtgcttttct cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg
    20341 cagttcttta ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc
    20401 agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc
    20461 gcggatttca atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt
    20521 tactgcatct tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat
    20581 caattctgac ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt
    20641 gctgcattga ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg
    20701 caatctgtca agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta
    20761 ctcgccttta gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc
    20821 ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga
    20881 caggctgatt gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc
    20941 aacgctcaga accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag
    21001 gtaaccttca acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac
    21061 tttaccggtt acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc
    21121 tgcgagctgt ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac
    21181 tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca
    21241 ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc
    21301 gaagtcagtg atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc
    21361 gaactgctgc cacgggttag ctttgcactg tttcagaccc agggagatac gacgacgttc
    21421 ttcgtcgata tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg
    21481 gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt accaggcctt caacgccttc
    21541 ttcgatttca acgaagcagc cgtagtcggt caggttggtc acgcgaccag tcagtttggt
    21601 accttccgga taacgtttag cgatagctac ccacggatct tcgcccaact gtttcaggcc
    21661 cagggataca cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc
    21721 cacgttgacg atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag
    21781 caggccgtca acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat
    21841 acctttaact tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt
    21901 ttcggattcg ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt
    21961 gattacttta aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac
    22021 gtctaccaga gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc
    22081 cttaactttg ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag
    22141 cgtgatccag gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc
    22201 gtcttctact gcgtccagag caacgtcaac ttcatcacct acctggattt ccagctcgcc
    22261 ctgggcgttt ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac
    22321 cagtactacg tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt
    22381 ttcgatttct tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct
    22441 tcaggtttat atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg
    22501 ctgtcattcc attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc
    22561 tggcgcgcgt attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc
    22621 aacactaaag catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg
    22681 tcgcgttctt tgatctcggc caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc
    22741 aactgtagca tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact
    22801 ggcgcatcag ggaataccac cgttcccatg tcgcggccat cggcaatcag gccgggtaat
    22861 tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact
    22921 tgtgaagctg cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg
    22981 aggatcactt ccagattgcc attggttgac acaaaacgta catccagatg ggatgccagc
    23041 ggtaccagcg catcttctga cgcaacatca acatggtgat gtaatgccgc caatgccagt
    23101 acgcgataaa ttgcacccga atccagcaga tgccattgca atgcttccgc catagcctta
    23161 cacaaggtgc ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc
    23221 gtcatcttta tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc
    23281 cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg
    23341 atttattgcg caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta
    23401 cttaccagag gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga
    23461 gtcaaacatg gactgggtgc gaccaccatc cagactagcc agtttttcaa agctgccaac
    23521 cagcccctgg gtgctgatgc cacgtttctt caacagatca aaggagaaat catctgcttc
    23581 ggactcttta tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg
    23641 tgagcgggaa agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac
    23701 gatcgcataa gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc
    23761 atggcccaga acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac
    23821 acggacacag ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata
    23881 gttgaccggc gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt
    23941 accgtacttg ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg
    24001 attggcaatc gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc
    24061 ggagctgata gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc
    24121 tgcagaggtc gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt
    24181 aatcatatat aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc
    24241 aaaatgactc acaaggtccg aaaaaaaacg ccgacaatgt gccgacgttt tgataaagaa
    24301 agatttggct atttattgcc cgttgttcat tcaggctgcc tggctaatcc gcgccaactg
    24361 ctcgaaataa tccggaaatg ttttggccgt acatttggga tcaagaatcg tcactggtgt
    24421 atctgacaac gccaccagcg agaaacacat cgccatccgg tgatcattgt acgtcgcgat
    24481 ctcggcaaag ttcagttttt ccggtggagt gatacgaatg taatcgtgcc cctcttctac
    24541 ttccgcaccg actttacgca gttctgttgc catcgcaaac aggcgatcgg tttctttaac
    24601 acgccagtta tagatattgc gcagcgtggt ggtgcctttt gcaaataacg ccgccgtggc
    24661 aatggtcatc gccgcatcgg gaatatggtt catatccata tcaatagcgt tcagttcacc
    24721 acgcgtgcag gaaatataat catcgcccca gcaaatggtc gcgcccattt tttccagcac
    24781 atcagcaaag cgaatatcac cctgcatact gttacgtcca ataccggtca cttttacagt
    24841 gccgcctttg attgctgctg ctgccagaaa gtaagaagcc gaagatgcat cgccttcgac
    24901 caaataagta cccggagact gataagactg cccgcctttt acgacaaatt gttgatagtg
    24961 ctgattttca atttcaacac caaacgtctt catcagattg agtgtgatgt cgatataagg
    25021 tttagaaacc agatcgcctt taatacgaat caccgtatct tccggcgcaa gaggcgcagt
    25081 cattaacagt gcggtgagga attggctgga aacggagcca tcaacgtcaa cgttgccgcc
    25141 agtaaagccg ccctgtaaac gcaacggcgg ataattttct tgttccaggt aagtgatctt
    25201 cgccccgccc aggcgcagcg catccaccag atgaccaatc gggcgttctt tcatacgcgg
    25261 ctcaccggtc agcacaatat cattgctacc cagacaaaga gctgccgcca gcggacgcat
    25321 tgccgttccg gcgttaccga ggaacaactc cagggcacct tctgcgtgta atggaccgcc
    25381 gttaccgata atttcgcaac gcgtacgatc ggctgaaagc gtatagctta cccctaacgc
    25441 tgttaatgca ttcagcatat ggcgcacgtc atcgctatcc agcagattgg ttaatactgt
    25501 tttgccgtgt gctaatgccg ccagcaataa agcgcggtta gaaacgctct tggaaccggg
    25561 cagattaata gtgccatcga cacgagcgat gggttgtaac gtcagggatt ccatgaactc
    25621 aactctctac aacagaaata aaaaccccac aggctggctg tggggattaa gcaaaatttc
    25681 ggcattaacc gtgacggcgt tcgaactcaa ccatgaagtc tgtcagcgct ttaacgcctt
    25741 ccagcggcat ggcgttataa atagaagcgc gcattccgcc gaccacacgg tgacctttca
    25801 gtgcatgaag gccagcagca aaagactctt caaggaacaa tttgtcaagc gcactgtccg
    25861 ccaactggaa cggcacgttc atccgcgaac ggttagcttt cgccacgtca ttgcggtaga
    25921 aatcgctgtt atcaatcacc ccatatagca gttctgcttt ttgctgattg attttatcca
    25981 tttcagctac accgccgttc gctttcagcc atttaaagac cagaccagat agataccagg
    26041 caaatgtcgg cggcgtgtta aacatggagc cgttatcgtt gaggatggaa taatccagaa
    26101 tcgacggaca cgcgatattc gctttgccca gcaaatcttc acgaacgatg acgattgtca
    26161 ggccagccgg gccgatattt ttctgcgcgc cagcgtaaat cacgccataa cggctgacgt
    26221 caatcggacg ggaaagaatg gttgaagaga agtcggcggc gaccaccaca tctttgccga
    26281 agtctggcgt ttcgtcgatg gcgataccgt cgatggtttc attcgggcag taatgcatat
    26341 aagcagcatt atcagagagt tgccattcac gcattggctt aaccgcgcgc agaccatcaa
    26401 cagtcacttt ggcgtcaaag acattaggcg tgcagtattt tttcgcttct ttaatggcac
    26461 ttgccgccca gtaaccggca tcaacataat ctgcggtggt tttatcaccg agaatattca
    26521 gcggtaccgc agcaaactga ccgcgaccac cgccatggca gaataatacc ttgtagttgg
    26581 aggggacatt aagaagatcg cgaaaatcct tctcggcttc ctctgcaacc tgaatgaact
    26641 ctttgccacg gtgactcact tccatcaccg acgtaccaag accgttccag tcgcgcagtt
    26701 cctgttgagc ctgtttaagc acctctgccg gtagcattgc cggaccagaa ctaaaattga
    26761 agatttgagc catttcccct caccacgttg cgttgctatt aacccgctgt caatcgattg
    26821 cgaccacagg taatacatta ttcctgtggc tatcggtttt atcattcagt gacacacatc
    26881 gcaatgagta aaaccgttga gggcaaaaat gtggcttgca tcacacaaaa gaatctctcg
    26941 gattattagc aaaaagccga tgtttttact gtcaggtcac ccgttttgcc cgacttgcct
    27001 tcgtccattc tggatttgca cagcgcggac ataattgttt ttcccccgct ttcatatgaa
    27061 tgatttcact acaacgaata caggcatagt ccgccgactc cggcaccact ttgtaacgct
    27121 gcgtacaatc actgggtaaa ataagtaaac ccggtttcac cttgtcatct tcaaagaaat
    27181 agacactgat ttgcccgttg gtttcgagaa tcgccagacg tacctgcccc agctgctcca
    27241 cgccacgcaa tcgtagctcc ataaagaact caaattccgt catgttggag ttattgagtt
    27301 tcgaccaggc cagctcgcca tcttcaataa taacaaccgg cttaccttcc agtagatctt
    27361 ccagtttttc actgtgcgcc atcaaccaca ttaccaggcg gtataacaac gccagagtga
    27421 taaagacgat aagtaccggc accatcggca catcatcata aaacgccaca tctcccgccg
    27481 ctgaacccag cgtgagaatg attaacactt caaacagcga catctgtcgc acaccgcgtc
    27541 ttccggtcat tttgaggaac aaaaaaacca gtacaaaggt ataaagacta cgtagtgcaa
    27601 cttcgccaag gaaatcaaaa ggcactttat caaatgccat acggtggaga tcgaacgcct
    27661 tcatttttat gctcttatcg tggtttatcc gctaaaagca tagtcaacag ttcatcggca
    27721 tggcggcggt gatagcgcct ttgagataaa acccgtatta ttgcgcgctt tccgtacgac
    27781 taaagtgatt ttcatgacgc aaacatttat ccccggcaaa gatgccgctc tggaagattc
    27841 catcgctcgc ttccagcaaa aactttcaga cctcggcttt cagattgaag aggcctcctg
    27901 gctgaatcct gtgcctaacg tctggtctgt acatattcgc gacaaagagt gcgcactgtg
    27961 ttttaccaac ggaaaaggcg caaccaaaaa agcggcgctg gcttctgcac ttggtgaata
    28021 tttcgagcgt ctctcaacca actacttttt tgctgatttc tggctgggcg aaaccatcgc
    28081 caacggtccg ttcgtgcatt atcccaacga aaaatggttc ccactgaccg aaaatgacga
    28141 tgtgccagaa ggactactcg atgaccgtct gcgcgcgttt tatgatccgg agaatgaact
    28201 gaccggcagc atgctgattg acctacaatc cggtaacgaa gatcgtggta tttgcggcct
    28261 gccgtttacg cgtcagtccg acaatcagac cgtttatatt ccgatgaata tcattggtaa
    28321 cctgtacgtc tccaacggta tgtccgcagg taatacccgc aacgaagcac gcgttcaggg
    28381 attgtctgaa gttttcgaac gctacgtgaa aaaccgcatt attgctgaaa gcatcagcct
    28441 gccagagatc ccggcagacg tgctggcgcg ttacccagca gtagttgaag ccatcgaaac
    28501 gctggaagcg gagggtttcc caatcttcgc ttatgacggt tcgcttggcg gccagtatcc
    28561 ggtgatttgc gtggtactgt tcaatcctgc taacggtacc tgctttgcct ctttcggtgc
    28621 gcatcctgat tttggcgtag cactggaacg taccgtgacc gagctgctgc aaggtcgtgg
    28681 cctgaaagat ttggatgtgt ttactccgcc aaccttcgat gatgaagaag tcgctgaaca
    28741 taccaacctc gaaacgcact ttatcgattc cagcggttta atctcctggg acctgttcaa
    28801 gcaggatgcc gattatccgt ttgtggactg gaatttctcc ggcaccacgg aagaagagtt
    28861 tgctacgctg atggctatct tcaacaaaga agataaagaa gtttatattg ccgattacga
    28921 gcatctgggc gtttatgctt gccgtattat cgtgcctggc atgtccgata tttatccggc
    28981 tgaagatctg tggctcgcga ataacagtat gggcagccat ttacgtgaaa cgattctttc
    29041 gctaccaggc agcgagtggg aaaaagaaga ttacctgaac ctcatcgagc aactggatga
    29101 agaaggtttt gatgacttta cccgcgtgcg tgagctgttg ggtctggcga ccgggtcgga
    29161 taacggttgg tacaccctgc gtatcggtga attaaaagcc atgctggcgc tggctggtgg
    29221 cgatctggaa caggctctgg tctggaccga atggacgatg gagtttaact catcagtatt
    29281 tagtccggaa cgcgccaact attatcgctg cctgcaaacg ttgttattac tggcacagga
    29341 agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt cgcatgtacg gcgcagatgc
    29401 cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg ttttacggcc tgcaaccggt
    29461 agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg ttgaaggcct acgaaaagct
    29521 gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa atagcctacg caatgtaggc
    29581 ttaatgatta gtctgagtta tattacgggg cgttttttta atgccccgct ttacatatat
    29641 ttgcattaat aaaataattg taattataag gttaaatatc ggtaatttgt atttaataaa
    29701 tacgatcgat attgttactt tattcgcctg atgctccctt ttaattaact gttttagcgg
    29761 aggatgcgga aaaaattcaa ctcatttgtt aatttttaaa atttattttt atttggataa
    29821 tcaaatattt actccgtatt tgcataaaaa ctatgcgagt tacgggccta taagccaggc
    29881 gagatatgat ctatatcaat ttctcatcta taatgctttg ttagtatctc gtcgccgact
    29941 taataaagag agagttagtg tgaaagctga caaccctttt gatcttttac ttcctgctgc
    30001 aatggccaaa gtggccgaag aggcgggtgt ctataaagca acgaaacatc cgcttaagac
    30061 tttctatctg gcgattaccg ccggtgtttt catctcaatc gcattcgtct tctatatcac
    30121 agcaaccact ggcacaggca caatgccctt cggcatggca aaactggttg gcggcatttg
    30181 cttctctctg gggctgattc tttgtgttgt ctgcggagcc gatctcttta cttccacagt
    30241 gttgattgtt gttgctaagg cgagtgggcg catcacctgg ggtcagttgg cgaaaaactg
    30301 gctaaatgtc tattttggca acctggtcgg cgcactgctg tttgtacttt taatgtggct
    30361 ttccggcgag tatatgaccg caaatggtca atggggacta aacgtcctac aaaccgccga
    30421 ccacaaagtg caccatactt ttattgaggc cgtctgcctt ggtatcctgg caaacctgat
    30481 ggtatgtctg gcagtatgga tgagttattc tggccgtagc ctgatggaca aagcgttcat
    30541 tatggtgctg ccggtcgcga tgtttgttgc cagcggtttt gagcacagta tcgcaaacat
    30601 gtttatgatc ccgatgggta ttgtaatccg cgacttcgca tctccggaat tctggaccgc
    30661 tgtcggttct gcaccggaaa atttttctca cctgaccgtg atgaacttca tcactgataa
    30721 cctgattccg gttacgatcg gtaatattat cggcggtggt ttgttggttg ggttgacata
    30781 ctgggtcatt tacctgcgtg aaaacgatca ccattaatgg ttgtcgaagt acgcagtaaa
    30841 taaaaaatcc acttaagaag gtaggtgtta catgtccgag cttaatgaaa agttagccac
    30901 agcctgggaa ggttttacca aaggtgactg gcagaatgaa gtaaacgtcc gtgacttcat
    30961 tcagaaaaac tacactccgt acgagggtga cgagtccttc ctggctggcg ctactgaagc
    31021 gaccaccacc ctgtgggaca aagtaatgga aggcgttaaa ctggaaaacc gcactcacgc
    31081 gccagttgac tttgacaccg ctgttgcttc caccatcacc tctcacgacg ctggctacat
    31141 caacaagcag cttgagaaaa tcgttggtct gcagactgaa gctccgctga aacgtgctct
    31201 tatcccgttc ggtggtatca aaatgatcga aggttcctgc aaagcgtaca accgcgaact
    31261 ggatccgatg atcaaaaaaa tcttcactga ataccgtaaa actcacaacc agggcgtgtt
    31321 cgacgtttac actccggaca tcctgcgttg ccgtaaatct ggtgttctga ccggtctgcc
    31381 agatgcatat ggccgtggcc gtatcatcgg tgactaccgt cgcgttgcgc tgtacggtat
    31441 cgactacctg atgaaagaca aactggcaca gttcacttct ctgcaggctg atctggaaaa
    31501 cggcgtaaac ctggaacaga ctatccgtct gcgcgaagaa atcgctgaac agcaccgcgc
    31561 tctgggtcag atgaaagaaa tggctgcgaa atacggctac gacatctctg gtccggctac
    31621 caacgctcag gaagctatcc agtggactta cttcggctac ctggctgctg ttaagtctca
    31681 gaacggtgct gcaatgtcct tcggtcgtac ctccaccttc ctggatgtgt acatcgaacg
    31741 tgacctgaaa gctggcaaga tcaccgaaca agaagcgcag gaaatggttg accacctggt
    31801 catgaaactg cgtatggttc gcttcctgcg tactccggaa tacgatgaac tgttctctgg
    31861 cgacccgatc tgggcaaccg aatctatcgg tggtatgggc ctcgacggtc gtaccctggt
    31921 taccaaaaac agcttccgtt tcctgaacac cctgtacacc atgggtccgt ctccggaacc
    31981 gaacatgacc attctgtggt ctgaaaaact gccgctgaac ttcaagaaat tcgccgctaa
    32041 agtgtccatc gacacctctt ctctgcagta tgagaacgat gacctgatgc gtccggactt
    32101 caacaacgat gactacgcta ttgcttgctg cgtaagcccg atgatcgttg gtaaacaaat
    32161 gcagttcttc ggtgcgcgtg caaacctggc gaaaaccatg ctgtacgcaa tcaacggcgg
    32221 cgttgacgaa aaactgaaaa tgcaggttgg tccgaagtct gaaccgatca aaggcgatgt
    32281 cctgaactat gatgaagtga tggagcgcat ggatcacttc atggactggc tggctaaaca
    32341 gtacatcact gcactgaaca tcatccacta catgcacgac aagtacagct acgaagcctc
    32401 tctgatggcg ctgcacgacc gtgacgttat ccgcaccatg gcgtgtggta tcgctggtct
    32461 gtccgttgct gctgactccc tgtctgcaat caaatatgcg aaagttaaac cgattcgtga
    32521 cgaagacggt ctggctatcg acttcgaaat cgaaggcgaa tacccgcagt ttggtaacaa
    32581 tgatccgcgt gtagatgacc tggctgttga cctggtagaa cgtttcatga agaaaattca
    32641 gaaactgcac acctaccgtg acgctatccc gactcagtct gttctgacca tcacttctaa
    32701 cgttgtgtat ggtaagaaaa ctggtaacac cccagacggt cgtcgtgctg gcgcgccgtt
    32761 cggaccgggt gctaacccga tgcacggtcg tgaccagaaa ggtgctgtag cgtctctgac
    32821 ttccgttgct aaactgccgt ttgcttacgc taaagatggt atctcctaca ccttctctat
    32881 cgttccgaac gcactgggta aagacgacga agttcgtaag accaacctgg ctggtctgat
    32941 ggatggttac ttccaccacg aagcatccat cgaaggtggt cagcacctga acgttaacgt
    33001 gatgaaccgt gaaatgctgc tcgacgcgat ggaaaacccg gaaaaatatc cgcagctgac
    33061 catccgtgta tctggctacg cagtacgttt caactcgctg actaaagaac agcagcagga
    33121 cgttattact cgtaccttca ctcaatctat gtaattagat ttgactgaaa tcgtacagta
    33181 aaaagcatac aataaaggct ccacgaaagt ggggcctttt ttagcacgag agcctttttt
    33241 gtcagctatc tatactttaa ggtgactgcc aaaacagact cgacgtagcc ttcgagctgc
    33301 gcaccaacac ggcctcagat gggccacatc tggagaaaca ccgcaatgtc agttattggt
    33361 cgcattcact cctttgaatc ctgtggaacc gtagacggcc caggtattcg ctttatcacc
    33421 tttttccagg gctgcctgat gcgctgcctg tattgtcata accgcgacac ctgggatacg
    33481 catggcggta aagaagttac cgttgaagat ttaatgaaag aggtggtgac ctatcgccac
    33541 tttatgaacg cttccggcgg cggcgttacc gcatccggcg gtgaggcaat cctacaagct
    33601 gagtttgttc gtgactggtt ccgcgcctgc aaaaaagaag gcattcatac ctgtctggac
    33661 accaacggtt ttgttcgtcg ttacgatccg gtgattgatg aactgctgga agtaaccgac
    33721 ctggtaatgc tcgatctcaa acagatgaac gacgagatcc accaaaatct ggttggagtt
    33781 tccaaccacc gcacgctgga gttcgccaaa tatctggcga acaaaaatgt gaaggtgtgg
    33841 atccgctacg ttgttgtccc aggctggtct gacgatgacg attcagcgca tcgcctcggt
    33901 gaatttaccc gtgatatggg caacgttgag aaaatcgaac tcctccccta ccacgagctg
    33961 ggcaaacaca aatgggtggc aatgggtgaa gagtacaaac tcgacggtgt taaaccacct
    34021 aagaaagaga ccatggaacg cgtgaaaggc attcttgagc agtacggtca taaggtaatg
    34081 ttctaatttc cccgtaaagc ggcaacttta ttgagttgcc gcttttttat tccgcgcttc
    34141 attctgctac agcgttttta acacatttta tagtgcatct accatatccc gtacctgaga
    34201 taataacgac tgataatagc tcgcatgaag ttcttgccca tcgaatacca ctgtattaca
    34261 caagaaagtg acatcggcat cttcaatacc aagataactg cacatatttt tcaaataatc
    34321 gcttatattt ttttcccagc ccatctggac aaatgattct ttgtctcctc caaccagcgc
    34381 cacccaacga actttattga atggtaattt gtgcccatct ccataagcca gcccattatt
    34441 ccatactctg tcaatatatc cttttagcat tgccgggaag ctataccacc agagaggaaa
    34501 aaccaccact aacgtgtcat gttcaagcag ctctgaataa agctgatgaa cctctggaga
    34561 ataacgttta tccatattct tccagtctgg ttcatcttcc ggcgtcatca ctgggttgaa
    34621 attacgccga tataaatcga gttccgtcac ctgtattttc cgctccattg cccgctgatg
    34681 gatcgcatca gcaatatttg cggtcaatga atcatgacga ggatgggccc ataccaaata
    34741 aatacgttca gactgcataa ttttacctcc acgttaagtt ctggagcgca ctttacgcag
    34801 gcggcataat cctgtaaacg tgatgattca ggatgagtta atgaaggatt ttcactaatg
    34861 cggatgaata tgtctgactt tgccactttc tttgccgtcg cccgtaatca aagctttcgt
    34921 gcagcgggcg atgagttagg cttatcctcg tccgccatta gccatagtat taaaacactg
    34981 gaacaacgtc ttaaaattcg cttattcaat cggacaaccc gaagcgtatc gttaacggaa
    35041 gcgggttcta atctctatga acgattgcgc ccagctttcg acgaaattca gatcatgtta
    35101 gatgagatga atgattttcg tctgacaccg acaggaacgc tcaaaatcaa tgccgcccgg
    35161 gtagctgcac gtatattttt aatgccttta ctggttggtt tcacacgtga atatcctgat
    35221 attaaagtcg agctcacaac tgacgattct cttgtcgata tcgtccaaca gggctttgac
    35281 gctggcgttc gacttagtgg catcgtcgaa aaagatatga tcagcgtggc gatcggccca
    35341 ccagtgaaat tatgtgtagc agcaacgcca gagtactttg caagatatgg caaaccccgc
    35401 catcctcatg atctgcttaa tcatcaatgt gttgtttttc gttatcccag cggcaaacct
    35461 ttccactggc aatttgcaaa ggagctggaa attgctgtcg caggaaatat cattctggat
    35521 gatgttgatg ctgaactgga ggccgttttg atgggtgctg gtatcgggta tctgctctat
    35581 gaacagatca aggagtacct tgatactggt cgtttagaat gtgtactggg agactggagc
    35641 acagagcgtc caggcttcca gatctattat ccaaaccgcc agtatatgtc ctgcggcttg
    35701 cgcgcatttc tcgattatgt aaaaacgggt cagatctgcc agagtcagcg tcaccgacca
    35761 caataattat ttactattcg ctttacgcgc cagcagcggg aaaatcagtc ctaatcctac
    35821 cagcacaaac ggcgttgcaa cattcagcgc caactggaag gtccactctg cagtgaaggc
    35881 ttccattttc gggaagatcc ccgtcaaaca ggcaaaggcg gtaaaggcaa aacaccaaat
    35941 cccgacggtc attgccagag gcttattacg aataaagaca tattctggtt tatatttctg
    36001 cgccaggcga acgactgcaa taaatgcaac aaatacccat aaataacgca gcggcatcac
    36061 taccgagtta agattcaaca gccatttata gagattgttc atatcaccga tgccgagagt
    36121 cggcagcata atcagaattg ccaccagcac cagggtcaga aaatagccat taacgggcgt
    36181 accagaagcg ttggtacgac ataaactggc tggaatatat ttgctgtcag catcacctaa
    36241 tagcacttta agcggggcat caatcgagaa taccagcgca gctacttgtc ccagggtatt
    36301 cgcaatggcg taaatcacca ttaaagtatt acccatgttg taatactcgc ccagcttctg
    36361 aaaggcgtaa tattgaccgt tagtcattaa gtcatccggg atattacgcg agtcaaacat
    36421 catccccatc gccagtgagc ccagaatggc acaaaccgca accatcaccg ccaggcatag
    36481 catccctttt ggaaattctt ttcctgggtt gcgcgtttga ttaacgtaag gagaaatctt
    36541 ctctgctccg ccaaccgcga aaaccagcat tgaaatagtg gtaatgtagg taaagtcgat
    36601 atgaggaatg aacgtttccc aggtaatgtt tgtggtcgca atatgcactt cagtaatcgc
    36661 agacgcggtt accgccatcg ccacatacag gagcgacata acaaacatcg caatccctgc
    36721 cacagaaccg acgattttca gcgatttcat accgcgtgaa gcaacccaca taaagaagat
    36781 aaacagcacc agcgttaacc cctgtaacgc tacgactgaa tattctttga ttagcgaacc
    36841 gtcgcctttc atcgcccaac cgagcgcaat cagaattgcc tggggttttt gtgccagaaa
    36901 gggaatatgc accacccagt aggtccacgc ggcgagataa gccagtccgg gtcccatcgt
    36961 gtgtttaatc caggtactga ccccgccctt cccatctttg aaggtcgagc ctaactgacc
    37021 aacaattagc gcataaggtg tgaaatagag tgcaaagata aacacccatg aaaaaacaac
    37081 caccagcccc tggttggcat agttattaac aacgttgcca aaaccccaga cagtgataaa
    37141 agacatcagc gcaatgttgt accatcgcaa ctgtttttcc tgaacattcc cagccataga
    37201 caaatccttc gccaaaatta aaggggcatc tgtaaaacga gggattatgc gcggcaaata
    37261 agaagtgcgg aaatagcgaa atgcaaaatt ataacctgct gcacatttta tttttaaacg
    37321 aataaaattt ggaataaagc aagtcagata gaagaaaaaa aaagcagagt gtgaaacagt
    37381 aagttcacac tctgcttgaa ttcatttaca cgtgagcaac gggtttcggc gtatgaccgg
    37441 cgttgcgcag cagcatcagc aaatagataa acgatacgct ggcgatcatg ataaacaata
    37501 aattatcgga gaaattctgc attagcatag cggtaaatga cgggccaagc agacttccca
    37561 cagtatagct caacagtaag gcctggttca tcgccaccag ttgatgatgt tcaactttct
    37621 cgcaagccca tgccatcgcc accggatata gcgtaaagcc agcggcaccg aggatgaata
    37681 acgctggggc catcgccgcc tggctaagca tcgcgatact gccgagaatg acgacaaaga
    37741 cctgaacacg caacaccagc agtcgaccaa acttatccgc cagacgtcca atcggccatt
    37801 gtccaaggat acccgcactg accagtaccg ccatccagaa accaatgctg gcattgctca
    37861 cccctttgtg attgaggtac agcggcatca ggccatatag agatcccaga acgattcctg
    37921 agataatgca gccattcacg ccaagccgcg cctgacggag ttttagcatt gacgtaattg
    37981 acgtcgaatc atggttttca gcctgctgat tcagcacacg cgtaaacaac agcggtaaga
    38041 tccctgccaa cgtcaaacct gtaacccacg gcaatacgga catcagctcg gttgaaactt
    38101 tgctgaccag taactggcct aaaaacgttc ccacgtaata aaccatcata tacgcagcaa
    38161 gcaaacgccc acggttacgt gacgtcccac tgcacatcag cgcgctctca acaaccaccc
    38221 aaatcatggc acagccgacg cccgcgacaa aacgccaagc caaccagctc cagaatccaa
    38281 tcatcaggcc aaggccggca cagccagcgg caaaaatgaa ggaggccaga taatagctgc
    38341 ggttaaagcc aatgcgctta atgacatacc ctgtcagcaa tgtaccgaca aggttgccgg
    38401 taaaatagga tgagctgaca acgcctacct gccatgtgga catgtgttcc tgggcgagcc
    38461 aaagcggcac gagtgtattt aacaccgcga tcgccagagt caacaaaagc aggccagaca
    38521 gcaaaagcat gacaggccgg gtatacgtgg acatgaatta aaaccgtgag gaagttcaaa
    38581 tttcatgcgc atcatgccac cgccaaaaac attgtcaatg ggtgaaaata cgcgcatatc
    38641 gcagcggcgg tattatcgat ttatattact cattcataac gctaatgctg aagagatgat
    38701 taatctcatc tgattgtttt tattgatcta acacaaattt catacgcttc gttcagtcga
    38761 aaaatttcat ggtcacagca aactcatatt tcccgtctat gctttcagag caatgcatca
    38821 cgtctgaccg atttcagacg taccactgac tctcgaaagg agaagaggat gaccaaaccg
    38881 tatgttcgtc ttgataaaaa tgatgctgcc gttttgcttg ttgatcatca ggctggttta
    38941 ctttcccttg tacgggatat cgaacccgat aagtttaaaa ataacgtgct ggcgctgggt
    39001 gacctggcta agtatttcaa cttacctact attcttacca ccagttttga aaccggtcct
    39061 aacggtccat tagttccaga actgaaagca caattccccg atgcacctta cattgctcgc
    39121 ccgggaaata ttaacgcctg ggataacgaa gattttgtaa aagcagtcaa agcgacaggt
    39181 aaaaaacagt taattattgc cggtgtggta accgaagttt gcgtggcatt cccggcgctt
    39241 tcagccattg aagaagggtt tgatgtcttt gttgtgactg acgcgtcagg gacctttaat
    39301 gaaattaccc ggcattcggc atgggatcgc atgtcgcaag caggtgcgca attgatgaca
    39361 tggttcggcg tggcgtgcga gctgcaccgt gactggcgta atgatattga aggactggcg
    39421 acgttgttct ctaaccatat tcctgattat cgtaatttga tgaccagtta tgacacgtta
    39481 acgaagcaga aataaaagag aaccggggcc gaaaccccgg ttgttactga ttagcttgcg
    39541 acggccatcc ccacggtcat atgcaaacca tagaatacgc cgcgaccgat taattcccct
    39601 gccagaagca gaatgaatga gacggatagt agcggaaccg caggctgata accttttagc
    39661 tgtggtgcaa tccacaagca cagggcaacg gccaaaagca cgatccgcca ggacatcagc
    39721 gcaccatagt ccggcaccag cgctgcggcc tgctgcacag aactatgaat agttgccagc
    39781 tctgtgccct gcatcactga catcacgcca ctcaccacca gtgccagtac agaaatggct
    39841 ggcagcagac gcatcgccca gccatcgacg ccagccagac tcaacaacag gtaacccagt
    39901 aacgggccgc ccataaacat cgtcaggaag aagcccatcg gcgtccagat gctgtaccag
    39961 gtcggaacgg tatcaatgct gttatacaca cgcaccatca tccagacaaa gatgacgcca
    40021 agaaccatcg tcactatcag ccacagcgta cgcaatgccg gtgacagctt tttcagcatt
    40081 gccagcaacc agccgatgcc gcctacggca aaaaagatcg aaccgctggc gatttcgtta
    40141 ctgagtgctg aagcccctac ccggttgagc gagttaaaag cgcgcattgg tgaaccaaga
    40201 tggagcatag aggcgataaa gccaatgccc atcagcaccc ataaaccaaa catgcaggcg
    40261 ataacacgct gctgggcttc tgctcgcagg tcgcctttga gcagcgccaa agccagaacg
    40321 ataaaaccac ctgctacaca ttgcccgaag accgtgaaga tcatcagcgg ccattcatgc
    40381 catccacttc ccatctcaca cctccttcgg gtttgccaga tagccagtgg tatccccggt
    40441 cgggcggcta ttggcattgg gtttgatcac aatattcggt ttggtaaagt gagctcgcgg
    40501 caacggcgca acggccgcca gatcgccatg ttttttacgc agctcgtcga tagggccgaa
    40561 atccagcgcc cgcagcggac aagattcaac acagatcggc tttttaccct ccgcaacacg
    40621 gtcataacaa ccatcgcatt tggtcatatg gcctttcgtt tcgttatatt gcggtgcgcc
    40681 atacgggcaa gccatatggc agtagcggca gccaatgcac acatcttcat cgaccacaac
    40741 aaaaccatct tcacgtttat gcatcgcacc gctcgggcag actttagtac aagccggatc
    40801 ttcgcagtgg ttacatgaaa tcgacagata gtaggcaaac acgttctggt gccagacacc
    40861 gttatcttcc tgccagtcgc cgccagcata ttcataaatg cggcggaagc tgacttctgg
    40921 cgtcaaatct ttgtagtctt tacaggccag ctcgcaggtt ttgcaaccgg tgcaacggct
    40981 ggaatcaata aaaaatccat actgggttgt catcggttac tccttacacc ttttcaacct
    41041 gaacaaggtt tgtatgtgac ggattcccct tagcgagagg agacggacgt tgagtggtca
    41101 gtacgttgat acaaccgccc ttatcgacac gttttgtatc cgggtcatac caggcacctt
    41161 cacccagtgc gaccacaccc ggcatcattc gtggcgtcac tttcgcctca atatgaacct
    41221 caccacgatc gttaaagatc ctgactttat cgccgttgtt gataccgcgt ttttgcgcat
    41281 caagcgggtt gatccacatt tcctgacggc aagctgcttt cagcacatca acgttgccgt
    41341 aagttgagtg aacgcgagat ttatagtgga aacccgtaag ctgcagcgga tactgtttgt
    41401 tcagcggatc ctgataactt tcaaagcccg gcgtgtagat cggcagtgga tcgatcacat
    41461 cgccttctgg caattcccag gtagcggcaa tgtcagccag cgcctgcgaa taaatctcaa
    41521 ttttgcccga tggcgtagtc agtgggtttg cctgcggatc ttcacggaag gctttataag
    41581 caacgtgatg cccttgtggg tcgcgctttt taaagatccc ctgcttgcgg aactcttcaa
    41641 acgttggcag ttcaggaatc gcttcccgcg actgggcata cagatgccgc atccactctt
    41701 cctgggtacg gccttcagta aactgttgct caacgccaag acgttttgcc agttcgctgg
    41761 tcatttcata gatggtctta cattcaaagc gcggtttaat cacctgatcg ttgaaaatca
    41821 cgtaagacat attcccgcag gatgcatcca gtgcaaagtc catctgttcg gaagcggtgc
    41881 agtcaggcag caggatgtca gcatatttcg ccgatgaggt catgtggcag tcgataacca
    41941 caatcagctc gcacttctta tcatcctgaa ggatttcatg ggtacggttg atttcagaat
    42001 gctggttaat caggcagtta ccggcatagt tccagatcat tttgatcggc acatccagct
    42061 tatctttccc gcgtacacca tcacgcagcg ccgtcatttc cgggccacgt tcaatggcat
    42121 cggtccacat aaacatcgaa atgctggtct ggatcgggtt ttccaaggtc ggcatacgga
    42181 caaacggtaa gctgtatgaa ccttcacgcg cgccgctgtt gcctccgtta ataccaacgt
    42241 taccggtcag aatcgccagc atcgagatag cacgggttgc gatttcaccg ttagcgtgac
    42301 gctgcgggcc ccatccctgg ctgataaacg ccggtttggt actgccgatt tcacgagcca
    42361 gtttgatgat tttgtctgcc ggaacaccgg tgatttgtga ggcccattcc ggtgttttag
    42421 ccacgccatc tggcccttca cccagaatat aagctttata gtggccattt ttcggcgcac
    42481 tggctggcag ggttttctca tcgtagccaa cgcaatattt atcgaggaat gcctgatcca
    42541 ccaggttttc agtgatcatg acgtacgcca gaccgttaac cagtgcggca tctgttcccg
    42601 gacgaatagg gatccactca tcttcgcgcc cggcaccggt gtcggtatag cgcggatcaa
    42661 tgatgatcat gcgggcatta gatttctggc gtgcctgttc aagatagtaa gtcaccccac
    42721 cgccactcat tcgcgtttcg ccagggttat taccaaacag cactaccagc ttactgtttt
    42781 cgatatccga cgggctgttg ccatctgccc agccgccgta ggtatagttc aaaccttccg
    42841 caatttgcgc ggaggagtag tcgccgtaat ggttcagata gccgccgcag cagttcatca
    42901 gccgcgcgac cagggtattt cccggcggcc aggagcgggt catggtgccg cccagcgtac
    42961 cggtgccata gttcagatag atagactcgt tgccgtactc tttgatcagg cgctgcatat
    43021 tggtcgcgat gatgtcgtag gcttcttccc agctaatgcg ctcgaatttg ccttcaccgc
    43081 gcgccccgac tcgtttcatc ggatatttca ggcggtccgg attgtagaca cggcgacgca
    43141 tggaacgccc acgcaggcag gcgcgaacct ggtgcaggcc gtcgtaattg tcatcgccgg
    43201 tattgtccgt ttcgacatat ttgatttcac cgtccacgac gtgcatacgt agcgggcagc
    43261 gactaccaca gttaactgta caggcgctcc agataacctt ttcgtctgat tttgttggaa
    43321 tggcgctatc gacagcgtgc gcaatccgac taaaaggtaa tgttaatgcg ctgctggcca
    43381 ttgccaggcc gccgatcgct gtcgttttta ccaaaccacg gcgactcacc tcagcagcca
    43441 ataccgcatc agggattttc gttttcataa tggctcactc aagcttgctc acgattgatt
    43501 ccagtaacaa accgtattgt gtaattattt ctatagcagc tcgctatata ggcttgtata
    43561 catcgaataa tttgaaaatc aaagagatag gtagattttc actcgaaagt agtattacgt
    43621 gtaagtgagg agtaattatt gttcggtatc aaagggagac aaaaaaagcg cccgcaggcg
    43681 ctttttagat tcagaaaaat tgggtattag ccaatatatt ccagtccgtt catatacgga
    43741 cgcagaactt ctggtacttc aatacgacca tcagcctgct gatagttttc cattactgca
    43801 accagcgtac gaccaacagc cagtccagaa ccgttcaggg tatgaaccag acgggttttc
    43861 ttgtccgact tgctgcggca gcgtgcctgc atacgacgcg cctggaaatc ccaaacgttg
    43921 gagcaggaag agatctcacg gtaggtgttc tgtgccggga tccatacttc caggtcgtaa
    43981 gttttgcaag cgccaaagcc catgtcgcca gtgcaaagga tgattttacg gtacggcagg
    44041 cccaacaact gcaggacttt ttctgcatga ccagtcatct cttccagcgc cgccattgag
    44101 tcttccgggc gcacgatctg caccatttca actttgtcga actggtgcat acggatcaga
    44161 ccacgggtgt cacgaccata tgaaccggct tcagaacgga agcatggggt gtgggcggtc
    44221 atcttaattg gcagatcatc ttcatcgatg atttcaccgc gcaccaggtt agtcagcgga
    44281 acttctgccg ttgggatcag cgcatagtta ctggtgtctg cttcttcttc cagcggacga
    44341 gtatgaaaca gatcgccagc aaatttcggc agctgacccg taccgtacag cgtgtcctgg
    44401 ttaaccaggt acggaacata gttctcactg tagccatgct gttcggtatg cagatccagc
    44461 ataaactgcg acagtgcgcg gtgcatgcga gcaatctgcc ctttcattac cacaaagcgg
    44521 gaaccagtca gcttaactgc agctgcaaag tcgaggccag agtgcatttc acccagcgtc
    44581 acatggtcac gaacttcaaa gtcaaactca cgcggggtgc cccagcggct gacttcgacg
    44641 ttgtcatttt cgtctttacc taccggcact tcatctgcag gcaggttagg gatggtcagc
    44701 gcgatatcgc gaatttcagc ctgtaaagca tccagctcgg cttttgctgc atccagctct
    44761 tcgcccagtt tgttcacttc cagacgtaaa ggctcgatat cttccccgcg cgctttcgcc
    44821 tggccaatgg atttcgatcg ggagttacgc tccgcttgca ggttttccgt tttgacctgc
    44881 aatactttac gacgctcttc aagagcgccc agcttatcta catccagctt aaagccccgg
    44941 cgtgccagtt tttcagcgac tgcgtctggc tcattacgca gcagattggg atcgagcatg
    45001 cttatcctgt gcttatcgaa ttaaatggga gatgtggcca cagcctgcga ccacagggat
    45061 acagtaacaa cattaccgca acgataacat taacggtagc gttttatggg gctattttga
    45121 tcctgttcag ccagccaggc gagcttttcg ccaatcttgc cttcaaggcc cctgtttgtc
    45181 gggaaataat agcgtgtttg tgctatttcc ggcgggaagt aaacctcacc ggcagcataa
    45241 gcgtttgctt catcatgagc gtaacgatat tcctgcccgt agcccatttc cttcattaat
    45301 ttcgtcggcg cattacgcaa atgaaccggc acgtcataat ccggacgttc gcgagcatcc
    45361 gccagcgcag ctttaaacgc agtgtagaca gcgttgcttt ttggcgcgca ggccaggtaa
    45421 acaatcgcct gagcaatggc gcgttcacct tccgccgggc caacgcgagt aaagcaatcc
    45481 caggccgcaa ttgccacctg catcgcccgt gggtcggcat tgccgacatc ttcggaagca
    45541 attgccaggc aacgacgcgc gacatataac ggatcgccac cagcggtaat aattcgcgca
    45601 taccagtaga gcgccgcatc gggtgcgcta ccacgtaccg acttatgcag tgcggaaatg
    45661 agatcgtaaa agcgatcgcc tttgttatca aagcgggcgc ttcgctcacc ggcgatttcg
    45721 gtcaataatt caggcttcag gacccgctta ccgctatcat tgacttcggc catatccgcc
    45781 atcatttcca gcgtatttaa cgcccggcgc gcgtcgccat tcaccagttc agcaatggcg
    45841 cgtcgtgttt catctggcag aacaatatcc tgaccaccat aaccacgggt cttgtcttcc
    45901 atcgcctgag ttagtacttg ctcaatatcc tctgtaccca gggatttcaa cagatagaca
    45961 cgggcacggg aaagcagtgc cgaattaagc tcaaacgacg ggttttcagt ggttgcgcca
    46021 ataaaagtga tggtgccgtc ttcaatatgt ggcagaaatg catcctgctg gcttttgttg
    46081 aatcggtgca cttcgtcaac aaaaagaata gtgcggcgac ctgcattgcg gttttgccgg
    46141 gcgcgctcga tcgcctcgcg aatctctttc acgccagagg tgacggcaga aatacgttcc
    46201 acatcagcgt tcgcatagcg ggcaatcact tcagcgaggg ttgttttgcc ggtacccggt
    46261 ggcccccaga ggatcataga atgtaaatgc ccggcttcga tagcgcgcgg caacggcttc
    46321 cccgcagcca gcaaatgttg ctggccgata tactgtgcta aattttctgg ccgcatacgc
    46381 gcggccagag gttgaaaagt attatccgaa aaatcgagcg acagattgct cactcaggtg
    46441 cctctactta cgttgatcat ctaccgtgac gccttgcggc ggggtgaagg taaatttcgc
    46501 tgcatccaca gccccatttt gctgggattt cagttgataa ctgctgcgct gatcgtcctg
    46561 ctccaccgcg ctaaactgat ggattgtgcc atcacgtccc acgttaatgg tgaactgctt
    46621 cagattgcca ttgctggctt tcggcgtcag gacaaagtca tcgccattct gtttgatatt
    46681 gtactgctgc cagtcgctgg actggttgcg ggcaatcagc ataaacggcg tattaccggt
    46741 ggcatctttc agccaggttg ccgtagcttg ctcaacgaac gggttataga accacagtgt
    46801 tttaccgtca gaaaccagaa tgctttcatc aggttgtgtc atatgccagt tgaataagtt
    46861 tggacgtttc acccacagat cgccctgacc ttcctgcacc gccgcgccgc taccgtcagt
    46921 cactttttgt gtgaagctgg cgtggaagct gctgacttta tccaggcggc ttttcagatc
    46981 gcttgcggca tcagcccaaa cgctgcttgc tactaagctt gagagtaatg cacaggtgat
    47041 ggcaattttt ttcatcatta ttcctcaaat tacgtcactc ccgatcggga gttccctcta
    47101 ctctctattc caggctaata atcagcttga ggaaagaaga aaatactgaa ttttcagttg
    47161 atagagtgcc ggatgcgacg ctaccgcgcc ttatccggca atgcattaat tagtcaaacg
    47221 gcggtggggc cagcacttca cgattaccgt tgtgcccctg ttcgctgaca atcccctgcg
    47281 cttccatctg ttcgataata cgcgctgcac ggttataacc aatgcggaac tgacgctgta
    47341 cgccagaaat tgacgctttg cgtttttcag tgacaaactg caccgcctga tcgaacaacg
    47401 gatccagttc ttcagcgcca tcgaaaccgc ccgcgccacc ttcgctttca ctgtcggaag
    47461 tgatgccatc aacatactgt gggcgaccac gcgctttcca gtcctgcacc acggcatgaa
    47521 cttcctgatc gcgaacaaaa gcaccatgga cacgtaccgg caacgtggag ttcggcccag
    47581 agtagagcat atcccccatc cccagcagtg attccgcgcc agcctgatca agaatggtac
    47641 gtgagtcaat cttactggat acggtaaagg cgatacgggt cggaatattc gctttaatca
    47701 gaccagtaat aacatcaacc gatggacgct gagttgccag tacgaggtgg atacccgcgg
    47761 cacgggcttt ttgcgccaga cgtgctatca gctcttccac ttttttacct accgtcatca
    47821 tcaggtcggc aaattcgtca accaacacca caatgtatgg ttcttttttc agcaccggat
    47881 gctgggcatc catactgtca cccggcttcc agtacgggtc tggaatcgga cgcatcatgc
    47941 gatcggcttc agcaattttt tcgttataac ccgccagatt acgcacaccc agcgcagaca
    48001 tcagtttata ccgacgctcc atctcgttaa cacaccagcg cagcgcgttg gcggcatctt
    48061 tcatatcagt aacgacttcc gttaacagat gcggaatgcc ttcataaacc gaaagctcca
    48121 gcattttcgg gtcgatcatg atgaaacgca catcttctgg ctgtgcttta taaagcatgc
    48181 tcaggatcat cgcgttcaca ccgacagatt taccggaacc ggtagtcccc gcaaccaaca
    48241 agtgcggcat tttcgccaga tcggcaacca ccggctcacc ggcgatatct ttacccagca
    48301 ccacggttaa tggcgacgga ttatcgcgga atttggcgtt atccaaaact tcgcgcagat
    48361 aaacggtttg tcgtttttta tttggtaact ccagacctac atagggtttg ccaggaataa
    48421 cttcaacgac acgcaccgcc accgtcgaaa gtgaacgggc aaggtcccgt gacaagttag
    48481 aaatgcgcgc cgcttttacg ccaggagcca ggttaagttc aaagcgagtg ataaccggcc
    48541 ccggagagta attgacgaca tcggctttaa tacggaaatc agccagacgc gcttccacca
    48601 ggcgagccat ttgttcaagc gcaaaggtat ctaccggctc cacttcgctc ggcggcggtg
    48661 tcagcaaatc cagagaaggc agcggcgtcg tcggtttatg caacggacgg ctgtcgccat
    48721 tacgcatcaa cagcggatga agcagggtat cctgctgctg cggtgcaacc ggttgttgcg
    48781 gctgttgata ctgcggctgc ggagcaactg gttgttgcgg ctgctgatat tgcggctgtg
    48841 gcgcaaccgg ttgttgcggc tgctgatatt gtggctgcgg cgcaacctgc tgttgtggct
    48901 gctgatactg cggctgcggc gcaaccggct gttgcggctg ctgatattgc tgctgtggcg
    48961 gaactggttg ttgcggctgc tgatactgct gctgcggtgc aaccggttgt tgcggctgct
    49021 gtacaggttc aacaattggc gtaaacaacg gttcgtgtgg accatcatcc agcaacgctt
    49081 tcattggcga aaattcaaaa tcatccagcg agaacggatt cgccccagcc ggttgttcgc
    49141 cggaataacg ttgttgttga gtttgcgcaa actgacgagc cagttcagcc tctgccgcag
    49201 catctgcatc ttctgcgttt acgggcacat catgttgata ctgttcgcca tagcgttgct
    49261 gctgtgtctg ggcgaactga cgtgccagtt catcctgctg catcgcatcg atttcatcat
    49321 cgttatactg atcgccagaa tcatactgat tgcgctgggc ttcacgggct ttctcttccg
    49381 ccgcacgctg tgagggcagc ttaataccgt aagacgccag ttcacgacga gttggcacgc
    49441 ggatacgttt cggtcgtggc aactgcgggc caatcccctc tttgacttgc ggacgcggtc
    49501 caccgctatt tgccagactg aagactggcg cggcaacggt tgcggcagcc cccgtcgcca
    49561 gtgtcgcttt tttcacgcca gatgccagcg gagaaacagc ggcagcggct tctactggag
    49621 gtactgctgc aacagaaggc gctttcaacg aagatttgat cggttctggt tctttaaccg
    49681 gttctggaat cggttgatac caggccgcaa gttgttcacg ttcacgggct cgcttctctt
    49741 ccacttcttc aaaatagtaa agcggcggac gcgcgggttt tgtctcttct acaacgggtt
    49801 caggctccac aacaggctgc tgttcaacgg attgcggctg ttggtacaac ggctcctgag
    49861 cggctggttg ctgataagtt tgctcagtct ggtatgtaga ctgtggagca aaagtggatt
    49921 gctgctcttc ggcttgccag gcgttacctg ccaccggttg ttctggcgca ggggcgtaat
    49981 acggctgttg tgcaggctgt tccggcgcag ttgcgtaata cggttgctga acaggttgtt
    50041 ccgccgcagg agcgtagtaa ggttgttgcg caggttgttc agctgcaggt gcataatacg
    50101 actgctgcgg ctgtactggt tgttgcagcg gctcattata ttgcactgca ggctgcgcat
    50161 attgtgactg ctgtgggtaa ccttccggtg caggagcaat aaccggctct cccgtttgtg
    50221 gacccggtac aggctgccag gctactgtag gttgcgaggg tggaacatca acagaggcaa
    50281 caggcggcgt ctgagtcaca ggttcaaccg gcgcagccca gctttgtgtc gccgtggtag
    50341 cagcagctgc tacagcgaca ggttcggtaa ttggcgcacc gtttaataat ggatcgtatt
    50401 cgtcatattc tggctgcgtt gcacgattgc ccgaaaatag gacgtcgtcc gggtcggcag
    50461 ccacaccgcg tgcagtgtag gtaatctctt cgtcatcatc catccgctta ccggagaaca
    50521 acgcagcgtc tgtttgccgc cccatcggat taatgaattt ttccgccaac cgtttacgac
    50581 gcgctagcgc gccgcgaaga atacgggcac ggcgtgattc atgctgtttg ccgtggtttt
    50641 catcttcata ctcttcgtca tcttcatatt catcttcatc gacccaggta tcatcgcgac
    50701 gggtacgatt actggcgaag gtgagaatgt ttaaaatcca gccgccgagt ttttcagcaa
    50761 tggtcaccca tgaccaaccg gtgaacagcg tcaggcccgc agcccaaacg cagagcagcg
    50821 caatagttcc cccgctactg tgtagcagtg gttgtagcgt agtacttagt aagctgccaa
    50881 tgacgccacc ggaggcaaaa taccagatat cgtcagcgtt gattgccgcc agaccacagg
    50941 aggtaaggat gagcgccaaa acgccaatga tgcgtagcga aacggcaaaa taatcaatgt
    51001 attcgtcgct ggactgatga cgccaggcaa accaacaacc gccgacaata atgacgggaa
    51061 tggtgtaagc catcacgcca aaaataaaga acagcgtatc tgccaaccac gcaccgggca
    51121 tcccacctaa attatggata ggttcatgcc aggccgtttg cgaccagctg gggtccgaag
    51181 ggttaaagct tagtaaggca gccatcaacc agacggcaaa caggacaata aggatcagca
    51241 acgcctccag aaggcggcgg ccgctactta actttgtcaa tgtgacttct ttgtcttcag
    51301 tgtattcctg gctcaagaaa ggctctccag gttacaggct tttcctgccc gatgctaaaa
    51361 cggacaacag taccaggtta ccccagtact gttgctgtat ggattaacag gagtgtaatc
    51421 aaaatacgcc gattttgcac ctgttccgtg ttagcgcgtc ttaataacca gacgattact
    51481 ctgcttgact tcttccataa caacgtatgt ccgtgtgtca ttgacgccag gcagacgcag
    51541 cagggtttcc cccagcaact tacggtaagc tgacatatcc ggcacgcgtg ttttcaacag
    51601 gtagtcgaaa tcaccagata ctaaatgaca ctcctgaatt tcttcaagtt tttgtacagc
    51661 ggtattgaat tgttcaaaca catccggtgc gccacgattc agagtaatct caacgaatac
    51721 cagaagtgat gcatccagat aatgggggtt cagcagcgcc gtatagccct gaataaaccc
    51781 ttgtctttcc agccgacgca cacgctcaag gcacggcgtt ggggaaagtc ccacacgttt
    51841 agaaagctcg acgttagaaa tacgcccatc cttttgcaac tcattaagaa tgttacgatc
    51901 gatacggtcg agatctttgc cagggcgctt cttgctatct accattatta ttgtctctct
    51961 gtattccttc cctactcctg tctgaccctg aaacatcact gttcagagtc gctacccgtc
    52021 acgatagacc gaaatcatga agattcacgg cgcgcaatgc ctgggtctac ggtgagaaga
    52081 ccgttgcctt gtggcagcta tcgacatcac gaatggcgtc tgtccacgcc atacgtagat
    52141 ttcactagca cggtaaacat ggtatttaca tgcatggtta tgcgcatata agaatactgt
    52201 ttttttcttc ctcgaatgtt ttcgcaaaac accaggggat tgtcaaagca aaacatcgat
    52261 ttttagtaca acatgccaga tattcattaa caggggcgtt tattcatcat ttaatcggct
    52321 tataaagcgg gaaatttaag tagcagaggt gataatgacg ggagaatttg acctgcttat
    52381 cgatgctgcc gattgcacaa attgttaaca aaatcgtcat actctttttt tacgtctgta
    52441 aattccctac aatcctgccc attgtctgcc aacaactatg gggatctcat gggcacgacc
    52501 aaacacagta aactgcttat cctgggttca ggcccggcgg gatacaccgc tgctgtctac
    52561 gcggcgcgcg ccaacctgca acctgtgctg attaccggca tggaaaaagg cggccaactg
    52621 accaccacca cggaagtgga aaactggcct ggcgatccaa acgatctgac cggtccgtta
    52681 ttaatggagc gcatgcacga acatgccacc aagtttgaaa ctgagatcat ttttgatcat
    52741 atcaacaagg tggatctgca aaaccgtccg ttccgtctga atggcgataa cggcgaatac
    52801 acttgcgacg cgctgattat tgccaccgga gcttctgcac gctatctcgg cctgccctct
    52861 gaagaagcct ttaaaggcag tggggtttct gcttgtgcaa cctgcgacgg tttcttctat
    52921 cgcaaccaga aagttgcggt catcggcggc ggcaataccg cggttgaaga ggcgctgtat
    52981 ctgtctaaca tcgcttcgga agtgcatctt attcaccgcc gtgacggttt ccgcgcggaa
    53041 aaaatcctta ttaagcgtct gatggataaa gtggagaacg gcaacatcat tctgcacacc
    53101 aaccgtacgc tggaagaagt gaccggcgat caaatgggtg tcactggcgt tcgtctgcgc
    53161 gatacgcaaa acagcgataa catcgagtca ctcgacgttg ctggtctgtt tgttgctatc
    53221 ggtcacagcc cgaatactgc cattttcgaa gggcagctgg aactggaaaa cggctacatc
    53281 aaagtgcagt cgggtattca tggtaatgcc acccagacca gcattcctgg cgtctttgcc
    53341 gcgggtgacg tgatggatca tatttatcgt caggccatta cttcggccgg tacaggctgc
    53401 atggcagcac ttgatgcgga acgctacctc gatggtttag ctgacgcaaa ataattttac
    53461 aaatcagtaa caaaagtaaa gaaggcgaca ccatgcgact atgggtcgcc tttatttttt
    53521 ccccgttgta acattgctct gcaaataatt ctgataactc acctgctaag cgtgcaatga
    53581 ataaatcccg tcaaaaagag ttaacccgct ggttaaaaca gcaaagcgtc atctcccaac
    53641 gttggctgaa tatttctcgt ctgctgggct ttgtaagcgg catattgatc attgcccagg
    53701 cctggttcat ggcgcgaatt ctgcaacata tgattatgga gaatattccc cgtgaagccc
    53761 tgctgcttcc ctttacgtta ctggttctga cctttgtact gcgcgcatgg gtggtctggt
    53821 tacgcgaacg ggtgggttat cacgccgggc agcatatccg ctttgccatc cgccgtcagg
    53881 ttctcgaccg tctgcaacaa gcagggccag cgtggattca gggtaaacct gcggggagct
    53941 gggcgacgtt ggtactcgag caaattgacg atatgcatga ttactatgca cgctacctgc
    54001 cgcaaatggc gctggcagtg tcggtgccgt tgctgattgt ggtggcgatc ttcccctcta
    54061 actgggctgc ggcgctcatt ctgctgggca cagcaccgtt aattccgttg tttatggcgc
    54121 tggttgggat gggggctgcc gatgctaacc ggcgtaactt tctcgctctt gctcgcttaa
    54181 gtgggcattt cctcgatcgc ctgcgcggca tggaaacatt gcgtattttt ggtcgtggtg
    54241 aagctgaaat tgaaagtatt cgttctgctt cggaagattt ccgccaacgg acaatggaag
    54301 tgctacggct ggcgttttta tcctccggca ttctcgaatt ttttacctcg ctgtcaattg
    54361 ctctggtggc ggtctacttt ggtttttcct atctcggcga gctggatttt ggtcactacg
    54421 ataccggtgt gacgctggct gcgggttttc tggccctgat ccttgcgcca gagtttttcc
    54481 agccattgcg cgatctcggt acgttttatc atgctaaagc ccaggctgtt ggtgcagctg
    54541 acagtctgaa aacgtttatg gaaaccccgc tcgcccatcc gcagcgtggc gaggtggaat
    54601 tagcatcgac cgatccggtg accattgagg ccgaggatct gtttatcacg tcgccggaag
    54661 gtaaaacgct ggccggaccg ctgaacttta ctttgccagc aggccaacgt gcagtgttgg
    54721 ttggtcgcag cggttcaggt aaaagctcac tgctgaacgc gctttctggt tttctctcat
    54781 atcagggatc attacgaatc aacgggatag aattacgcga tttatcacca gaatcatggc
    54841 gtaaacatct ctcctgggtt gggcaaaacc cacaattacc ggcagcaaca ttgcgggata
    54901 acgtactact ggcgcgacct gatgccagcg aacaagaatt acaagcagcg ctggataacg
    54961 cctgggtcag cgagtttcta ccgctcctgc cacaaggcgt tgatacacct gttggcgacc
    55021 aggctgcccg cctttccgtg gggcaggcgc agcgcgtggc ggtggcccgt gcgttactaa
    55081 atccctgttc gctattactg ttggatgaac ccgctgccag ccttgatgct cacagtgaac
    55141 agcgcgtaat ggaggcgctg aatgccgcct ctctgcgcca gacaacgtta atggtcaccc
    55201 accagttaga agatcttgct gactgggatg tcatttgggt tatgcaggat ggccggatta
    55261 ttgagcaagg acgttacgcg gaattaagtg tggctggtgg cccattcgcc acattactgg
    55321 cccatcgtca ggaggagatt taaatgcgcg ctttgctacc ctatctggca ctgtataaac
    55381 gtcataaatg gatgttaagt cttggtatcg tgctggcaat tgtcacgctg ctcgccagta
    55441 tcggtctgtt gacactttcc ggctggttcc tctcggcctc agcggttgcg ggggttgccg
    55501 gactgtacag cttcaactat atgctacccg ctgcgggcgt gcgtggcgca gcaatcaccc
    55561 gtactgccgg gcgttatttt gaacgtctgg taagtcacga cgcgactttc cgcgtgttgc
    55621 agcatctgcg catttacacc ttcagcaaat tgctgcccct ctcccctgcc ggactggcgc
    55681 gctatcgtca gggcgaattg ctcaatcgcg tggtggcgga tgttgatacg ctcgatcatc
    55741 tttatctgcg cgttatctcg ccgctggtgg gcgcttttgt ggtgattatg gtggtgacaa
    55801 tcgggttaag tttccttgat ttcaccctcg cctttaggct gggcggcatt atgttactga
    55861 cgcttttcct gatgccaccg ctgttttatc gtgcgggaaa aagcaccggg caaaatctga
    55921 ctcatcttcg cggacagtat cgccaacaac tgacggcctg gctgcaaggg caagctgagc
    55981 tgaccatttt tggcgccagc gatcgttatc gcacgcaact agagaataca gaaattcaat
    56041 ggctggaagc gcaacgccgt caatctgaac tgaccgcatt gtcgcaagcg ataatgctgc
    56101 tcattggcgc gttagcggtg atcctgatgc tgtggatggc gtctggcggc gttggcggca
    56161 atgctcaacc cggcgcgtta attgccctgt ttgtcttctg cgcgttagcc gcgtttgaag
    56221 cactggcacc agtaacgggt gcatttcagc atctggggca agtcattgcc tctgccgttc
    56281 ggatcactga tttaacggat caaaaaccgg aagtcacctt ccctgatacc caaactcgtg
    56341 ttgccgatcg cgtttcgctg acattacggg atgttcagtt cacttatccg gagcaatccc
    56401 aacaggcact taaagggatt tctcttcagg taaacgccgg ggaacatatt gcgattctcg
    56461 ggcgaaccgg atgcggcaaa tcaacactgt tacaactgct gacccgcgct tgggacccgc
    56521 aacagggaga gattttgctt aacgatagcc ccatagccag cctgaatgaa gcggctctac
    56581 gacagaccat cagcgttgtt cctcagcgag tgcatctgtt tagcgccacg ctgcgtgata
    56641 atctgttact cgcctcgcct ggcagtagtg atgaggctct ggcggagagc ttgcgtcgcg
    56701 ttggtctgga gaagctgctc gaggatgcag gtctcaacag ttggttaggt gaaggcggac
    56761 gccagctctc cggtggtgaa ctgcgccgtc tggctatcgc ccgtgcgctg ttacatgatg
    56821 cgccactggt gttgctggat gaacctaccg aaggcttaga tgccacaacc gaaagccaga
    56881 tccttgaatt gcttgcagaa atgatgcgtg agaaaacggt gttaatggtc acccatcgac
    56941 ttcgcggact ctctcgtttc caacaaataa tagtgatgga caacgggcaa attattgagc
    57001 aaggtactca cgcagagctg cttgccagac aggggcgtta ttaccagttc aagcagggtt
    57061 tgtaagctat tattgaacga tccgacttgc gtggagtttt gcggtcatgc gcctggttca
    57121 gctttctcgc cattcaatag ccttcccttc cccggaaggc gcattacgtg agcctaacgg
    57181 cctgctggca cttgggggcg atcttagccc tgcgcgcctg ttaatggctt accagcgtgg
    57241 tatttttccg tggttttctc caggcgaccc catcctctgg tggtcgcccg atccccgcgc
    57301 ggtgctatgg ccagaatcac tgcatatcag ccgtagtatg aagcgatttc ataaacgctc
    57361 gccctatcgt gtcacgatga attacgcttt tggtcaggtc attgaaggct gtgccagcga
    57421 tcgcgaagaa ggaacctgga tcacgcgtgg cgtggtcgaa gcctaccatc gccttcacga
    57481 actcggccat gcccactcca ttgaagtctg gcgtgaagat gagcttgtcg gcggtatgta
    57541 cggcgtggcc cagggaacgc tattttgtgg cgagtccatg ttcagccgga tggaaaatgc
    57601 gtctaaaacg gcgcttctgg tattctgtga ggaatttatc ggtcatggcg gtaagcttat
    57661 cgactgccag gtccttaacg atcacacagc atcgcttggt gcctgcgaaa ttccccgccg
    57721 ggattacctt aattatctca atcaaatgcg cctcggacga ttgccgaata atttctgggt
    57781 accacgatgc ttgttttcac cacaagaatg aatgttttcg gcacatttct ccccagagtg
    57841 ttataattgc ggtcgcagag ttggttacgc tcattacccc gctgccgata aggatttttt
    57901 cgcgtcaggt aacgcccatc gtttatctca ccgctccctt atacgttgcg cttttggtgc
    57961 ggcttagccg tgtgttttcg gagtaatgtg ccgaacctgt ttgttgcgat ttagcgcgca
    58021 aatctttact tatttacaga acttcggcat tatcttgccg gttcaaatta cggtagtgat
    58081 accccagagg attagatggc caaagaagac aatattgaaa tgcaaggtac cgttcttgaa
    58141 acgttgccta ataccatgtt ccgcgtagag ttagaaaacg gtcacgtggt tactgcacac
    58201 atctccggta aaatgcgcaa aaactacatc cgcatcctga cgggcgacaa agtgactgtt
    58261 gaactgaccc cgtacgacct gagcaaaggc cgcattgtct tccgtagtcg ctgattgttt
    58321 taccgcctga tgggcgaaga gaaagaacga gtaaaaggtc ggtttaaccg gcctttttat
    58381 tttgtgatat gtatgaagta ctttggaagt ataagtccgt aacttgtctc gatgtagata
    58441 tactgcacaa cttgtttaca gtatgagcaa acaataatta tcattaaaat tcttcttgac
    58501 cgttttactc caactcccta tagtagcgcc ccgttgccac ccatgaggtt tggtagcaaa
    58561 acaatttggt gaggtgtccg agtggctgaa ggagcacgcc tggaaagtgt gtatacggca
    58621 acgtatcggg ggttcgaatc cccccctcac cgccatattt aaagaagagc tcgtacgaaa
    58681 gtgcgagctt ttttttcgca ttttatctgt ctgtacggca gtgaacgcgt tcgcaaaggt
    58741 tttaccgcac cccgtcgatt tctaagctgc ctgtacggca gtgaactcaa atactacacg
    58801 tttagcccgt tgttaataaa gcaaaaccat cccattctcc tgcagcaccc ttttttatcc
    58861 ttaccttccc gcagcactaa ttatcaacaa cttaccatca tcattaaaaa agggtctgaa
    58921 cctcctccac cccataaaca aaaagtccgt aacctctttc gagattacgg acttgaccaa
    58981 cctacctaac aatcagatta atgcgctgct tccgccttgt gcttttgtgc actctggaat
    59041 ccgtaagtca gctcattttt ctctttatcc agcgcgacgg tgacctgacc gccgtccacc
    59101 agcgaaccaa acagcagttc gttggcgagc ggttttttca ggttgtcctg gatgacacgc
    59161 gccatcggac gtgcgcccat tgcccggtcg taacctttct cggccagcca gttacgcgct
    59221 tcctggctca cttccagaga aacacctttc tgatccagct gaacctgcaa ctcgacgatg
    59281 aatttatcca ccacctgatg gatcacgtcg gttgacagat gatcaaacca gataatgttg
    59341 tcgagacggt tacggaattc cggtgtaaag atcttcttga tctcctccat cgcatcggtg
    59401 ctgttatcct ggtggataag accaatggat ttacgctcag tttcacgtac cccggcgttg
    59461 gtggtcatca ccagcaccac gttacggaag tccgctttgc gtccgttgtt atcggtcagc
    59521 gtaccgttgt ccatcacctg caacagaata ttgaacacgt ccggatgcgc tttctcgatt
    59581 tcgtccagca acagcaccgc atgtggatgc ttgatgaccg catcagtcag caaacctccc
    59641 tgatcaaaac caacgtatcc cggaggcgca ccaatcagac ggctgacggt atggcgttcc
    59701 atatactcgg acatatcaaa gcgcagcagc tcaatgccca acgctttcga aagctgtacc
    59761 gtcacctctg ttttcccgac cccggtaggg ccggcaaaca ggaacgaacc aaccggttta
    59821 tgttcgtgac ctaaacctgc acgcgccatc ttaatggctt cagtcagcgc ctcaatggct
    59881 ttatcctgac cgaagaccag cattttcagg cgatcgccga ggtttttcag ggtatcgcgg
    59941 tcactctgag aaacactctt ctctggaatg cgcgcaatac gggccaccac ggactcaata
    60001 tccgccacat taacggtttt cttgcgtttg cttaccggca tcaggcgtgc gcgagcgccc
    60061 gcttcgtcga taacgtcaat ggctttatcc ggcagatgac gatcgttaat gtatttcacc
    60121 gccagctcta ccgctgcacg caccgctttt gcggtataac gcacgtcgtg gtgcgcttca
    60181 tacttcggtt tcaggccatt gatgatttga acagtttctt cgatcgacgg ttcagtaata
    60241 tcaattttct ggaagcgacg cgccagagca cggtctttct cgaaaatgtt gctgaactcc
    60301 tgataggttg tcgaaccaat tacgcgaatt ttaccgctgg agagcaacgg tttgatcagg
    60361 ttagccgcat cgacctggcc accagacgct gcacccgcac cgataatggt gtggatctca
    60421 tcaataaaca ggatgctgtt agtgtcctgc tccagctgct tgagcaacgc tttaaaacgt
    60481 ttttcaaagt cgccgcgata tttagtgccc gctaacagag aaccgatatc gagagagtaa
    60541 atcgtacagt cagccatcac ttccggcaca tcgccctgaa caattcgcca ggcaagacct
    60601 tccgcaatcg cggttttacc gacaccagat tcccccacca gcagcgggtt gtttttacgg
    60661 cgacggcaga gaacctgaat agcacgctcc agctccttct cacgaccaat cagtgggtcg
    60721 attccgccca cgcgcgcaag ctgattcagg ttcgtcgtga aattctccat acgttcctcc
    60781 ccaccagctt gttcttcgct gtttggctgg ctgccaggat cagaagactg tgtcggctcg
    60841 tctttacgcg tgccatgaga gataaagttc accacatcga gacggctgac ttcatgttta
    60901 cgcaacagat atgccgcctg cgactcctgt tcgctaaaga tagcgaccag aacgtttgca
    60961 ccggttacct cattgcgacc ggaggactgg acatggaaga ccgcacgttg cagtacacgc
    61021 tgaaaactca gcgtcggctg tgtgtcgcgc tcctcttcac tggcaggcag aacgggtgtg
    61081 gtttgttcaa taaaggcttc cagttcctga cggagcgcaa ccaaatccac agaacacgct
    61141 tccagcgcct cccgggcaga tgggttactg agcagcgcca gtaacaagtg ctcgacggtc
    61201 ataaactcat gacggtgctc gcgcgctctg gcgaaagcca tatttaaact gagttccagt
    61261 tcttgattga gcataggcac ctcccccaat ttttatgcct gcattcaggc tttttctagc
    61321 gtacacagca atggatgctc attctccctc gcgtacttgt tcaccatcgc cactttggtt
    61381 tctgcaacct cggcggtaaa gactccgcaa atggccttcc cctggtagtg aacagcgagc
    61441 atcaattgcg ttgcacgttc tacatcataa gaaaagaatt tttgtaacac gtcaataaca
    61501 aactccatcg gagtgtaatc atcattgact aatatcactt tatacataga tggcggtttt
    61561 agcgcgtcgc gaactttttc ttccgccagt tgatcaaagt ccagccagtc gttcgtttta
    61621 cccattgtca gttatcatct tcggttacgg ttatcggcag aacgccctgc cgctgacaag
    61681 agcttatttg cctgaccaat ctactccaca tgatagataa ctatcatcta tcgttggcat
    61741 cagcgacatc tgtcacattc ctgtcaatag cgttaactgc ttcaaatttt tgattcatac
    61801 ctgccccctg gcccctgtca aacgcttgac ggctcgccct aattctctaa attgtatttc
    61861 tagagttggc gaggttttga acagccccct ctctgacccc ggtttattcc atcttacttg
    61921 tataagattt gcgaaggatg tcgaagcatg gaaaagggta ctgttaagtg gttcaacaat
    61981 gccaaagggt ttggtttcat ctgccctgaa ggcggcggcg aagatatttt cgctcattat
    62041 tccaccattc agatggatgg ttacagaacg ctaaaagctg gacaatccgt tcagtttgat
    62101 gtccaccagg ggccaaaagg caatcacgcc agtgttattg tgcccgtcga agtagaagcg
    62161 gcagtcgcat agctcttctg tctcattgtg tacatcctaa aggcaaaatg ccagcccgat
    62221 cggctggcat ttttatctca aaaattactc tcgtgccaga gcatctactg gatccagtcg
    62281 tgccgcattt cgtgcgggta accatccaaa taaaatcccg gtgaccgtcg agcagagaaa
    62341 cgccagcagc agtgccaacg gtgaaaaacc aatctcccag ccgggtaaga aaagctgcaa
    62401 ggtgaaagca attaacagtg acagtgttat tcccaacgcg ccaccgacca ggcaaaccag
    62461 tacggcttcg atcaggaact gttgcaaaac atcgcttgct cgcgcaccta cagccatgcg
    62521 aatgccaatt tcccgcgtcc gctcggtcac tgacaccagc ataatattca ttacaccaat
    62581 accgcccacc accagcgaaa tcaccgccac cagcgtcaga aacagttgta aagtacgtgt
    62641 ggtcttttca acagttttca agacgccgtc catgttccag gtaaagaaat ccttctttcc
    62701 gtggcgcagt gaaagtaaac gcgtgagttg ctgttccgcc tcggcgctgt caaatccttc
    62761 ttttaccctg acagtaatgg agttcagcca cgactggccc ataactcgcc cggacatcgt
    62821 gctgtaaggt agccagacac gcagcacttt actgctacca aacatcgact gtttttcttc
    62881 cgccacacca atgactctgg cgggcatatt gccgaccaga atcacctcgc caaccacatc
    62941 tgctttatgg gggaaaagct ggcggcgagt attactgtcg agaaccacga cctgcgcacg
    63001 accgttcagc tgctcctgat taaaggtgtt tccttcactg aaggtcatgc catagacatt
    63061 aaaataatcg ccgctcacgc cattggcact ggcagcaaca tcaacattgt tataacgcag
    63121 gcgcaggttt tgcgagacgg caggtgtggc tgaggcgacc cacggttgtt tttggatggc
    63181 gattaagtcg tcgtacttca gcgcctgctg atattgcgga tcgtcatcgc caaaatcttt
    63241 cccgggatag acatcaatag tattcgtacc aatagaacga atatccgcca gcaccatttg
    63301 tttggcggcg tcacccacca cgacaatgga aaccaccgac gcaataccga taataatccc
    63361 cagcatggtc agtaaagtac gcattttatt cgctgccagc gcccgccatg ccatcgtcag
    63421 cgcctcgtta aaaccgctga caaactgccg ccagccagac accgtgttga caacaggctc
    63481 cgtcccgcca gcaacattca ctttttcaat ggcgggagga ttgcgcacaa tttcgccgtc
    63541 gcgaatttcg atcacccgct cggcctgagc agcgacctgc ggatcgtggg tgacgataat
    63601 caccgtatgc ccacgatcgc gcagctgatg caggatcgcc atcacctctt cgccagaatg
    63661 gctgtccagt gcgccggttg gttcatcggc aagaattacc tgaccaccgt tcatcaatgc
    63721 ccgcgcgatg ctgacgcgct gttgctgacc acccgaaagc tgtgccggat aatactctgt
    63781 acggtcttcc agccccagcc gttgcagcaa ctcctgggca cgaagcagtc gctgtttccg
    63841 ctcaagacca gcatagacgg cgggtacttc aacgttctgc tcggcggtta aatgcgaaag
    63901 taaatggtaa cgctggaaaa taaagccgaa atgctcgcgg cgcagttgcg ccagcgcatc
    63961 ggcgtccagt gtggcaacat cctgaccggc gacgcgatag gtgccgctgg tggccttatc
    64021 cagacagccg agaatattca tcagggtcga tttaccggaa cctgaagcgc caacaatcgc
    64081 taccatctca ccggcataaa tatcgagggt gatgcccttc agcacctcaa cctgctcatc
    64141 accggcagga tagctgcgac gaatatcctt taattcgagc aaaggcgtca ttgtgcagct
    64201 cctggtttgg cctcaccaat caccacttca tcgcccgctt caagcccttt gacaatctca
    64261 acatcggtat cgttacgtgc gccaatcgtc acttcacgct cgcgtgtttc accattacgc
    64321 aacaatttga ctttataacg attatcgcca accggatcgc ctaacgccga cagagggatc
    64381 gtcagcacat ttttcacatc ggtgagctga atatgcactt gcgcagtcat atccagccgc
    64441 agcaaaccat tggggttggg gacttcaaaa cgggcgtaat agaaaatagc gtcgttaacc
    64501 ttttccggcg tcggtagtac atccttgatt tgcccctcgt agcgcgtcag tggatcgcca
    64561 agtaccgtaa accaggcttt ttgccccggc ttcaggtgga ttacatccgc ttcagaaacc
    64621 tgcgctttta ccagcatggt gctcatatct gccagcgtca gaatgttcgg tgcttgttgt
    64681 gcggcaatca ccgtctggcc ttgcagagtg gtgatttgcg tgacttcccc ggccatcggg
    64741 gcaacgatgc gagtgtaatc gagattggtt ttagccgtat cgagagaggc ctgattgcgc
    64801 ttgatttgcg cgtcaatggt gccaatttgc gcctgtttca cagccatctc cgtcgcggcg
    64861 gtgtcgagat cctgctgtga aaccgcctgc gtttgtgcca gacgttgctg acgggaatac
    64921 gtcacccgcg ccagtttcag ctccgcttcc gcctgctgcc gctgcgcacg tagctccatc
    64981 agcgttgctt cgacctcctt aatctggttt tcagcctgtt caggatcaat aacccctaaa
    65041 agctggtctt tttttacttt atcgccaatc gccaccgaca gagttttcaa ctgaccgctg
    65101 acctgcgcgc ccacgtcaac cttacgcagc gcgtccagct ttccggtcgc cagcacgctt
    65161 tgctgtaaat caccggggcg cacaatcagt gtctgataag tcggcacggg tgcgttaaga
    65221 attctccata acgtaatcag tccggcgatg actatcacca gcgcaataac gtaacgcttc
    65281 ttcacggttt tccgcttttt cataaatatt ctgaatactc catatgccaa tccgggtcat
    65341 taacttcaac gaaatatcaa caaaacccta aaatttacgt tagtaatcac actattgatg
    65401 tttggttaag atacgcaatg gtgaaatcga cgtcatgtat aaccattgat ttcatgaata
    65461 tgtcgcagct aactgaacgg acctttacgc catctgaatc tctcagcagc ctgtcacttt
    65521 ttcttagtct ggcacgtgga cagtgtcggc cgggtaaatt ctggcatcgc cgtagttttc
    65581 gccagaaatt tttgctgcgc tcgttgatta tgccgcgttt aagcgttgag tggatgaacg
    65641 aactttccca ctggcctaat ctcaatgtgt tgttaacgcg ccaaccgcga ctgcctgtgc
    65701 gtctgcatcg cccttacctt gcggcgaatc ttagccgtaa gcaattgctg gaggcgttac
    65761 gttaccatta tgcgttactc cgcggatgta tgtcggcgga agaattcagc ttatatttga
    65821 atacccccgg gctgcaactg gctaaactgg aaggcaaaaa cggcgagcag ttcacgcttg
    65881 agctgaccat gatgatctca atggataaag aaggtgacag cacaatcctg ttccgcaaca
    65941 gcgaaggtat tcctctggca gagctcactt ttaccctgtg tgaatatcag gggaaaagaa
    66001 cgatgtttat tggcggactg caaggcgcaa aatgggaaat cccacatcag gaaatccaga
    66061 atgcgacgaa agcctgccac gggctatttc ccaaacgcct cgtgatggaa gcggcctgtc
    66121 tgtttgccca acgtttgcag gtagagcaga ttattgccgt cagcaatgaa acgcatattt
    66181 accgcagcct gcgttatcgt gataaagaag gcaagatcca tgccgattac aacgctttct
    66241 gggaatcggt tggcggcgta tgtgatgctg aacgccatta ccgccttcca gcacagatag
    66301 cacgaaaaga gattgccgaa atcgccagta aaaaacgggc tgaataccgt cggcgctatg
    66361 agatgctcga cgctattcag ccacaaatgg ccacgatgtt tcgcggttaa tccgcgcgac
    66421 cgcgcgccag ccacagcacg cgtgagaaca tttttttcag cagcgtcggt acggagtcca
    66481 caccacgacg tccggcctcc attgccactt caatggcaag atcgggtttg gaagagctat
    66541 ggatcgcttt cgagataatt ttgcgcagat tcatcggtac attttccggg atttgcgcca
    66601 cgcggtggaa cacatcggaa aatccctggc gatacataaa atgttccata tccagcgccg
    66661 gtaacgccgt taaatgttct cgttcggctt cccgatcgtt attcaacagg ctgcgtaccg
    66721 tagcggcata tttcttccct gcttcatcgc catcgaccag tacatgccat tcaatcccca
    66781 ttcggcgggc aaatttaacc agtggcttta gcccggactg ggcaaactca atgaccttga
    66841 tcccttcggc atcgaaatgg tgtccacact ggcgcgccag ttcattgata acccaggttt
    66901 ccgtttcccc ttccaccagc aaccagcagc gagcaaacag cgatgacgga cggttaaaac
    66961 gaatgtgaaa ggatatgcgt cggctatctt cggtactcaa gccactcggc cccagacgcc
    67021 aggcggcaac gcgcgaggac tcacgtacca gtcggcaaac atgctctacc ggcgttaacg
    67081 aaagcaactc acccgagttg gtggtggcaa tgcgctgcaa tggcagaaga ttcaacagat
    67141 gccaggcaac tgaaagcata atggggtgta aacgggtttc tggatcttca attagcaata
    67201 acggacgcgc gtctttatcc agtctcaatg tgccttttgc ctgcaataaa gtagcaaaca
    67261 ggccgagcaa aataacccga tacgagcgcc ctccaggtcg gtcaatcatc cggttgatga
    67321 tgtccagata gcgccagctt cgttgctcat tgctggcccg ccgccgcatt aaacgatatc
    67381 gcgcctgtcc agccccctgc tcagagaaat aatgctcaag caactgcacc attgcggaaa
    67441 gtccctgacg aatctgtcca tcagagagat tttgcggatg tgaggacaac tcacgggcga
    67501 ggaaatcgag ctggcgcgcg gtgacttcca cattgggaac atttggcacc gtgccgttac
    67561 gaatacggcg cataaaacgg gcatcacgca agcgcagcac cggcattaaa cgcaccagat
    67621 ggcgtgcctg atcgttaata tcctcgacat caatcggatg tccgtcttta tcgagaaaac
    67681 tgcgcagtgt catcacgctg ccgtcttccg cactctcccc ttccagacga taaaaaatac
    67741 ggtgatagcc atcggtgcat ggcgtccagc acgcttccag cggccgataa cggcgaaccc
    67801 gatgtcggcc tggcagcgat tcgcggaagg tcaaaataat atgcagatga tgttctcgcc
    67861 cgttgatatc tcccggcggg aaccagaaat cgtcgcgctc aaaatggtag agatctgatt
    67921 ctggcgatag cagcagggtt aaggcgtcca gcaagctgga tttaccccac gcgttctccc
    67981 caatcaggac gttgttttgt tcgagcatca acgacaaacg gttgataccg cgaaaaccca
    68041 caatttcaac gcgctcaaga atcatatccc ctccagaaat atgtgactta tcctttacgc
    68101 tggcagtata acgggaaagg ccttgctaat acgtgatgac atcacattta agcatggcaa
    68161 atgtctgaaa aatgagcgaa taatcgcgtt atactccctc ttgctgataa gtctgcaaaa
    68221 acaacaaaat caaataatta gcatgataaa ttatttttta ttacgcctaa aggattaaat
    68281 cttttgcttg cactacactg tcaatcagac aaatgactat atggggcaac ccgctgttgc
    68341 cctaaatcaa atttactgga tttatttatc tggtgaatga ctcgccttcg tttttaaaat
    68401 agccgcgcat tatttccgtc gtcacttttt tacgacgacc ctataaaacg accatatttt
    68461 tcacagggtc aatttttaat tgaggtggat atgttcagaa aattagcagc tgaatgtttt
    68521 ggtactttct ggcttgtttt tggtggctgt ggtagtgctg tactggccgc aggcttcccg
    68581 gaattaggca ttggttttgc cggcgtggcg ttggcgttcg gtctgaccgt tctgacgatg
    68641 gcctttgctg ttggtcatat ttctggtggt cattttaacc cggcggtcac tattggttta
    68701 tgggctggcg gacgttttcc ggcaaaagaa gtcgttggct acgtaattgc ccaggttgtc
    68761 ggcggtattg ttgcagcggc gctgctgtat ttaattgcca gtggtaaaac gggttttgac
    68821 gcggcagcca gcggttttgc ttctaacggt tatggcgagc attcaccagg cggttattcc
    68881 atgctttccg cgctggtagt tgaactggta ttgagtgcag gtttcctgtt ggtgatccac
    68941 ggcgcaaccg acaaattcgc gccggcaggt tttgcgccga tcgctattgg tctggcctta
    69001 accctgattc acttaattag tattccggtg actaacactt ctgttaaccc ggcgcgcagc
    69061 accgcggttg ctatcttcca gggcggctgg gcattagaac aactgtggtt cttctgggtg
    69121 gtgccaattg tcggcggcat tatcggtggt ctgatttacc ggaccctgct ggaaaagcgt
    69181 gattaattaa cgcagaaggc ctggcgtaat gtcaggcctt tttttaagat gttgttatat
    69241 gttgattcat catcttgttg cgctcctttc atgagctaag ccacatattg ccactggcgc
    69301 aaggagcgcg cagggggcgg ccaatcgccg ccgccccatg cacccccggg ctctggcgaa
    69361 caaaatcgcc gctgcgcggt gccctcgact tatcccttac ggctaccggg tcgggcgcga
    69421 ggtaacatcc ctgtaaaacg cgccctcagc ccacatccat gtgggctgcc ccggccttca
    69481 gggaacgcct cggcgatttt gacgccacca acatcggtgc agtttgtaat taaagcgcaa
    69541 gaatatcttt aaatgaaaac gaatactcta ctgctcctta tgcataacat ttccttttaa
    69601 ttgcaacttt actcgtcctt ccgctttcag tagtgtgtca tcgcgcattt cgacttcatc
    69661 tttgcaagga ctgggttttc atgttttctg ggctgttaat cattctggtt cccctgattg
    69721 tgggttacct cattccgctt cgccaacaag ctgcgttaaa agttattaat cagctattaa
    69781 gctggatggt ttaccttatt ctctttttta tgggtataag tctggcgttc ctcgataacc
    69841 tcgccagtaa cctgttggcg attctgcatt attctgccgt cagtattacc gttattttac
    69901 tgtgtaatat tgccgccctg atgtggctgg agcgaggcct gccgtggcgc aaccaccatc
    69961 agcaagaaaa actcccgtcg cgtattgcga tggcgctgga gtcgctaaaa ctgtgcggcg
    70021 tagtagtgat tggttttgcc attggtctga gtggactggc tttcttacaa cacgcgaccg
    70081 aagccagtga atacacgtta attttgctac ttttcctcgt tggtattcag ttgcgcaata
    70141 atggcatgac cttaaagcag attgtcctta atcgccgggg aatgattgtc gccgtggtgg
    70201 tggttgtcag ttcattaatt ggtggtttaa ttaacgcctt tattcttgat ctccccatca
    70261 atacctcgct ggcaatggcc tccggtttcg gctggtattc tctttccggt attttattga
    70321 ccgaatcttt tggtccggta atcgggagcg cggcgttttt taatgatctg gcccgtgaac
    70381 tgattgctat tatgttgatc cctgggctga ttcgccgcag ccgctctact gcactgggct
    70441 tatgcggtgc cacatcaatg gatttcaccc tgcccgttct tcaacgtact ggcgggctgg
    70501 atatggtccc ggcggcaatt gttcacggtt ttattcttag cctgttagtg ccgatcctca
    70561 tcgccttttt ctctgcgtaa tacctctctg gcggtagatt cctgccgcca aaattgcgct
    70621 aaatcaatct cccttaaagt tgcatgaaaa atccctttta tccccgcgtt aagcgtctta
    70681 accttaaaca tgtatattaa atataacttt aaaaggtgtg atcatgtttt gtgtgcaatg
    70741 tgaacaaact atccgtactc cggcaggaaa cggctgctca tacgcgcagg ggatgtgtgg
    70801 taaaacggcg gaaacttctg accttcagga tttactcatc gcggcgctgc aagggctttc
    70861 tgcctgggcg gtaaaagcgc gtgaatacgg catcatcaac cacgatgttg acagcttcgc
    70921 gccacgtgcc tttttctcaa ctctgaccaa cgttaacttc gattctccgc gtattgtcgg
    70981 ctacgctcgt gaagcgattg ccctgcgcga ggcgctgaaa gcgcaatgcc tggctgtaga
    71041 tgccaacgcg cgcgtcgata acccgatggc tgacctgcaa ctggtgagcg acgatctcgg
    71101 cgagctgcaa cgtcaggctg cagaatttac ccctaacaaa gataaagcgg cgattggcga
    71161 aaacattctc ggcctgcgtc tgctgtgcct gtatggcctg aaaggtgcgg cggcctatat
    71221 ggaacacgcg catgttctcg gtcaatacga caacgatatt tatgcccagt atcataaaat
    71281 catggcgtgg ctggggacct ggcctgccga tatgaacgcg cttcttgagt gttcaatgga
    71341 aatcggccag atgaacttca aagtgatgag cattctggat gcaggcgaaa ccggtaaata
    71401 cggtcacccg acgccaaccc aggtcaacgt caaagcgacg gcgggtaaat gcattctgat
    71461 ttccggtcac gatctcaaag atctctacaa cctgctggaa cagaccgaag gcacgggcgt
    71521 taatgtctac acccacggcg aaatgctgcc tgcgcatggc tacccggagc tgcgtaaatt
    71581 caagcatctg gtcggtaact acggcagtgg ctggcagaat cagcaagtgg agttcgctcg
    71641 tttccctggc cccatcgtga tgacctcgaa ctgcatcatc gacccaaccg taggcgctta
    71701 tgacgatcgt atctggaccc gcagcattgt tggctggcct ggcgtgcgtc atctggacgg
    71761 tgatgatttc tctgcggtta tcacccaggc gcaacagatg gcaggcttcc cgtacagcga
    71821 aattccgcac cttatcaccg tgggttttgg tcgccagacg ctgcttggcg ctgctgatac
    71881 gctgattgat ctggtgagcc gtgaaaaact gcgtcatatc ttcctgcttg gtggctgtga
    71941 cggcgcacgc ggcgagcgcc actacttcac cgatttcgcc accagcgtgc cggatgactg
    72001 cctgatcctg accctcgcct gtggtaaata tcgctttaac aaacttgagt ttggcgatat
    72061 cgaaggtctg ccgcgtctgg tagatgcagg tcaatgtaac gatgcttact cagcgattat
    72121 tctggctgtc actctggcag agaaactggg ctgcggtgtc aacgatctgc cgctgtcgct
    72181 ggtgctctcc tggtttgaac agaaagcgat cgtcattctg ctgacgctgc tttctctggg
    72241 cgtgaaaaat atcgtcaccg gcccgactgc acctggtttc ctgacaccag acctgctggc
    72301 ggtgctgaac gagaaatttg gcctgcgttc tatcaccact gttgaagaag acatgaagca
    72361 actgttgagc gcgtaaggag tttgtcgatg acgatgccaa cgaatcaatg cccgtggcgg
    72421 atgcaggttc atcacattac gcaagaaacg ccggatgtgt ggacgatttc cctgatttgc
    72481 cacgattact acccatatcg cgccgggcaa tatgcactgg tcagcgtgcg aaactcagcg
    72541 gaaacgctgc gtgcttacac catttcctcc acgccaggcg tgagtgaata tatcaccctg
    72601 accgtgcggc ggattgatga cggtgtcggc tcccagtggc tgacgcgcga tgtaaaacgc
    72661 ggtgattatc tctggctttc ggacgcgatg ggggaattta cctgcgacga taaagcagaa
    72721 gataaattcc tgttgctggc ggcaggctgc ggcgtcacgc cgattatgtc gatgcgtcgc
    72781 tggcttgcga agaaccgtcc acaggccgat gtgcgggtga tctacaacgt gcgtacgccg
    72841 caggatgtta ttttcgccga tgagtggcgt aactatccgg taacgctggt ggcagaaaat
    72901 aacgttaccg aaggctttat cgctggtcgt ctcactcgcg aactgctggc aggtgtacct
    72961 gacttagctt cacgtaccgt gatgacctgc ggcccggctc cgtatatgga ttgggtagag
    73021 caggaagtga aagcgctcgg cgtgacgcgt ttctttaaag agaaattctt caccccagta
    73081 gcagaagcgg cgaccagcgg tctgaaattc accaaactgc aaccggcacg agaattttac
    73141 gccccggttg gcaccacgct actggaggcg ctggaaagca ataacgttcc ggttgtcgct
    73201 gcctgccgtg cgggtgtttg cggctgctgt aagacaaaag tggtttccgg tgaatatacg
    73261 gtgagcagta caatgacgct gaccgacgcc gaaatcgctg aaggttacgt actggcctgc
    73321 tcctgccatc cgcaggggga tttggttctc gcataatcgc cttatgcccg atgatattcc
    73381 tttcatcggg ctatttaacc gttagtgcct cctttctctc ccatcccttc cccctccgtc
    73441 agatgaacta aacttgttac cgttatcaca ttcaggagat ggagaaccat gaaacaaacg
    73501 gttgcagctt atatcgccaa aacactcgaa tcggcagggg tgaaacgcat ctggggagtc
    73561 acaggcgact ctctgaacgg tcttagtgac agtcttaatc gcatgggcac catcgagtgg
    73621 atgtccaccc gccacgaaga agtggcggcc tttgccgctg gcgctgaagc acaacttagc
    73681 ggagaactgg cggtctgcgc cggatcgtgc ggccccggca acctgcactt aatcaacggc
    73741 ctgttcgatt gccaccgcaa tcacgttccg gtactggcga ttgccgctca tattccctcc
    73801 agcgaaattg gcagcggcta tttccaggaa acccacccac aagagctatt ccgcgaatgt
    73861 agtcactatt gcgagctggt ttccagcccg gagcagatcc cacaagtact ggcgattgcc
    73921 atgcgcaaag cggtgcttaa ccgtggcgtt tcggttgtcg tgttaccagg cgacgtggcg
    73981 ttaaaacctg cgccagaagg ggcaaccacg cactggtatc atgcgccaca accagtcgtg
    74041 acgccggaag aagaagagtt acgcaaactg gcgcaactgc tgcgttattc cagcaatatc
    74101 gccctgatgt gtggcagcgg ctgcgcgggg gcgcataaag agttagttga gtttgccggg
    74161 aaaattaaag cgcctattgt tcatgccctg cgcggtaaag aacatgtcga atacgataat
    74221 ccgtatgatg ttggaatgac cgggttaatc ggcttctcgt caggtttcca taccatgatg
    74281 aacgccgaca cgttagtgct actcggcacg caatttccct accgcgcctt ctacccgacc
    74341 gatgccaaaa tcattcagat tgatatcaac ccagccagca tcggcgctca cagcaaggtg
    74401 gatatggcac tggtcggcga tatcaagtcg actctgcgtg cattgcttcc attggtggaa
    74461 gaaaaagccg atcgcaagtt tctggataaa gcgctggaag attaccgcga cgcccgcaaa
    74521 gggctggacg atttagctaa accgagcgag aaagccattc acccgcaata tctggcgcag
    74581 caaattagtc attttgccgc cgatgacgct attttcacct gtgacgttgg tacgccaacg
    74641 gtgtgggcgg cacgttatct aaaaatgaac ggcaagcgtc gcctgttagg ttcgtttaac
    74701 cacggttcga tggctaacgc catgccgcag gcgctgggtg cgcaggcgac agagccagaa
    74761 cgtcaggtgg tcgccatgtg cggcgatggc ggttttagca tgttgatggg cgatttcctc
    74821 tcagtagtgc agatgaaact gccagtgaaa attgtcgtct ttaacaacag cgtgctgggc
    74881 tttgtggcga tggagatgaa agctggtggc tatttgactg acggcaccga actacacgac
    74941 acaaactttg cccgcattgc cgaagcgtgc ggcattacgg gtatccgtgt agaaaaagcg
    75001 tctgaagttg atgaagccct gcaacgcgcc ttctctatcg acggtccggt gttggtggat
    75061 gtggtggtcg ccaaagaaga gttagccatt ccaccgcaga tcaaactcga acaggccaaa
    75121 ggttttagcc tgtatatgtt gcgcgcaatt atcagcggac gcggcgatga agtgatcgaa
    75181 ctggcgaaaa cgaactggct aaggtaaaaa gggtggcatt tcccgtcata ataaggacat
    75241 gccatgattg atttacgcag tgataccgtt acccgaccga gccgcgccat gctcgaagcg
    75301 atgatggccg ccccggttgg ggacgacgtt tacggagacg accctaccgt taatgctctg
    75361 caggactatg cagcagagct ttccggtaaa gaagccgcca tttttctgcc taccggcact
    75421 caggccaacc tggtcgctct gctcagtcac tgcgaacgtg gcgaagagta tattgtcggt
    75481 caggccgcgc ataactatct gtttgaagcc ggtggcgcag cggtgctggg cagtattcag
    75541 ccgcaaccca tcgacgcggc tgccgacggc acgctaccgc tggataaagt ggcgatgaaa
    75601 atcaaacccg acgatatcca tttcgcccgc accaaattac tcagtctgga aaacacccac
    75661 aacggcaaag tgctgccgcg tgaatacctg aaagaagcat gggaatttac ccgcgagcgc
    75721 aatctggcgc tgcatgttga cggtgcgcgc atctttaatg ccgtggtggc ttacggctgc
    75781 gaactgaaag agatcacgca atattgtgat tcgttcacca tttgcctgtc gaaaggtctt
    75841 gggacgccag tcggttcatt actcgtcggt aatcgtgatt acattaaacg tgccatccgc
    75901 tggcggaaaa tggccggtgg cgggatgcgt cagtccggga ttctggctgc cgccgggatg
    75961 tatgccctga aaaataacgt cgcacggttg caggaagatc acgacaacgc cgcctggatg
    76021 gcggagcaac tgcgtgaagc aggcgcggat gtgatgcgtc aggacactaa tatgctgttt
    76081 gttcgcgtcg gggaagaaaa tgctgccgcg ttaggcgaat acatgaaagc gagaaacgtg
    76141 ctgattaacg cctcgccgat tgtccgcctg gtgacgcatc ttgacgtctc gcgcgaacaa
    76201 ctggcggaag tcgccgccca ctggcgcgca ttcctggcgc gttaaggaga gagacgtgcc
    76261 gcaacgcatt ttagttctcg gtgccagtgg ctacattggt cagcatctgg tgcgcacact
    76321 cagccagcaa gggcatcaga tcctggcggc ggcacgtcat gtcgacaggc ttgcaaagct
    76381 gcaactggca aacgtcagtt gccataaagt cgatctcagc tggccggata accttccggc
    76441 cctgttgcag gatatcgata ccgtctattt tctggtgcac agcatgggcg aaggcggcga
    76501 ttttatcgct caggagcgcc aggtggctct caacgtccgc gatgcgctac gtgaagtacc
    76561 agttaagcaa ttaatctttc tcagttcgtt gcaggccccg ccacatgagc agtcggatca
    76621 tctgcgtgct cgtcaggcta cggcggacat tcttcgtgaa gcgaatgtac ctgtgaccga
    76681 acttcgggcc ggaattatcg ttggcgcagg ttcagcggcg ttcgaagtca tgcgcgatat
    76741 ggtctacaac ctgccagtgt taacgccgcc acgctgggta cgttcacgca ccacgcccat
    76801 cgcgctggaa aacttgctgc actatctggt ggcgttgtta gatcatccgg ccagcgaaca
    76861 ccgcatcttc gaagccgccg gaccagaggt gctcagttat cagcaacagt ttgaacattt
    76921 tatggcggtg agcggtaagc gccgctggtt gatccccatc cccctcccca cccgctggat
    76981 ttcggtgtgg tttctcaatg tgattacttc cgtaccgccc accaccgcca gggcgttgat
    77041 tcaggggctg aaacacgatc tgctggcgga tgataccgcg ctacgtgcac tcatcccaca
    77101 acggctgatc gctttcgatg acgcggtacg tagcacgttg aaagaggagg aaaaactggt
    77161 caactccagc gactggggct acgacgctca ggcctttgcc cgctggcgac cagagtacgg
    77221 ttattttgcc aaacaggcgg ggtttaccgt taaaacgtcc gccagccttg ctgctttatg
    77281 gcaggtagtg aaccaaatcg gcggtaaaga gcgttatttc tttggcaata ttttgtggca
    77341 gacacgggcg ttgatggacc gcgcgatcgg tcataagctg gcgaaaggcc gcccggagcg
    77401 cgaatattta cagactggcg atgcggtgga tagctggaaa gtgattgtcg ttgaaccgga
    77461 aaaacaactt acgttgttat ttggcatgaa agcgccgggg ctgggacgac tgtgttttag
    77521 cctggaagat aaaggcgact atagtactat cgatgtccgc gctttctggc atccgcacgg
    77581 tatgccgggg ctgttttact ggttattgat gatccccgcg catctgttta tttttcgcgg
    77641 aatggcaaaa caaatcgcca gactggcaga acaaagcaca gattaatcga caaaacctgc
    77701 gttattcttt catgtttccc attgcatcat gggggaaatc acggaagaat gcgcacgtta
    77761 ttttctcaac aacagtggat tgatatgaag gtactggtta ccggcgccac cagcggctta
    77821 ggtcgaaacg cggtagagtt tttatgccag aaaggcatca gcgtgcgagc gaccggtcgc
    77881 aacgaggcaa tgggcaaatt gctggaaaaa atgggcgcag agtttgttcc ggcggatctg
    77941 accgagctgg tttcatcaca agctaaagtg atgctcgcgg gcattgatac gctgtggcac
    78001 tgctccagct ttacctcacc ctgggggaca caacaggctt tcgatctggc taacgttcgc
    78061 gccactcgcc gcctgggtga atggactgtc gcctggggtg tgcgtaactt tattcatatc
    78121 tcttccccct ccctgtactt cgattatcac caccatcgcg atattaaaga agattttcgc
    78181 cctcaccgct tcgccaacga gtttgcccgc agcaaagcag ccagcgaaga agtgatcaat
    78241 atgctttcgc aggcgaatcc acaaacgcgc tttactattc tgcgcccaca aagtctgttc
    78301 ggaccgcacg ataaagtctt tattccccgt ctggcgcata tgatgcacca ctacggcagt
    78361 attctgttac cgcatggcgg cagtgcgctg gtcgatatga cctactatga aaatgccgtg
    78421 cacgcaatgt ggctggcaag ccaggaagcc tgcgataagc taccttccgg gcgtgtgtac
    78481 aacatcacca acggcgagca tcgcacactg cgcagcatcg tgcagaagct gatcgacgag
    78541 ttgaatattg actgtcgtat tcgttccgtc ccctacccga tgctggatat gatcgcccgc
    78601 agcatggagc gtttaggccg caagtcagca aaagagccgc cgctgaccca ctacggcgtc
    78661 tccaagttga attttgactt tacgctggat attacgcgtg cgcaggaaga gttaggttat
    78721 cagccggtca tcaccctgga tgaaggtatc gagaaaactg ccgcctggct acgcgaccac
    78781 ggaaaactgc cgcgctaatc ctgcccgtat ttctccagca atgcttcggc aatcgcctga
    78841 gtttctgcat ccgcttcgcc gttatataac gtcgggcgga aatgcatctg gaatgccata
    78901 atcacgcgcc gctgctcgcg cggtgtcata tcaggtttaa cgtcataacc gtagcgcgcc
    78961 aaaagctcca gcaatgacgc ggtatctacc ggtgtgtgcg gcgcgcgccc ggcaaggtaa
    79021 aagttaaccc gctgcgcatc cggccaggca ccaatcccct gctgcgccag ttgctgccag
    79081 ggaaataatg gccccggatc gtctttgcgc tgcggtgcga tatccgcatg tgccactacg
    79141 ttttccggct tgatgtgata acgggcaata atatctttcg ccagtggaat aagcgcctga
    79201 atctgtgccg gttcaaacgg ggcaaaatat ttcactccgg ccgatttttg ccagccacgg
    79261 ttttccagct caatgccaat agaggtgtcg ttaaggcgcg ttgccccgcg ccaggcgcta
    79321 atccccgcat gccaggccag ttcttgttcc ggcaccagtt gccagatgcg cggtttaccg
    79381 ttgtatcgcg gtggtaccgc agggaccaga taatgcgagc tgacctgctt atcggtcagt
    79441 gtcgccagcg agctatcaaa atcatctgcg gtgtagtgga tcaccagcac tttaatgcgc
    79501 ggatacgccg cctgcgcctg gcgtcgggta tcaagctgat atccctcttt ctcgacaatg
    79561 cctttttcgc ctgcacaccc tgccagtaac agagcggcag cgatcagcca gaagactctt
    79621 ctcatcgacg cgttttcacc gccgtaccgc tgacgctaac catcagcata ctgccgtttt
    79681 gcccgaccgt ttcgtagtcg atatcaatac cgacgacggc atcggccccc agcgcccgcg
    79741 cctgggagcc taattcctca aaggcgatct cccgtgcttt acgcagttct ttttcatacg
    79801 caccggaacg tccgccaacg atatcgcgga taccggcaaa gaaatcacgg aaaatattgg
    79861 cacctaaaat cgcttcgccg gtcaccacac cgcaatattc aacgatgatc tggccttcca
    79921 gagttggggt tgtagaaaat tgcatgatcg atctccttgt ttgttaagcc ttcgatctct
    79981 aaagcattat cagactgata cgctattatt gaaaggatat cattattatc gtagaacgct
    80041 ttcagagcga tcgcttataa ggaaatcatt atgcgctaca gcaaattgac aatgcttatc
    80101 ccctgcgcac tgctcctcag tgcctgcacc acagtcactc cagcttataa agataacggc
    80161 acacgcagtg gtccttgcgt tgaaggaggc ccggataacg tggcccagca attttatgac
    80221 taccgcattt tgcaccgcag caacgacatc accgccttgc gcccttatct gagcgacaaa
    80281 ctggcgacac tgcttagcga tgcctcccgg gataataacc atcgggaact actgaccaac
    80341 gatccattct ccagtcgcac aaccttgcca gagagcgccc gtgttgccag tgcctcaaca
    80401 atcccgaatc gtgatgcgcg aaatattcct ctgcgtgtgg atctaaaaca gggtgatcag
    80461 agctggcagg atgaagtgtt aatgattcag gaaggccagt gttgggtgat cgacgatgta
    80521 cgctatttag gcggcagcgt tcatgccacg gcgggtacgc tccgtcagtc catcgagaat
    80581 cgctaacgcc ttgatcaacc aataaacccc gtaaattgtc cggcatttcg tgaggaatgc
    80641 cgacatttat gctcgccgac caccgccccc gttattttgt gctatgttta ttgaataatg
    80701 cgctttgctt ttaactttta aagcagaaat attgcataat tattctgtca aaggtactat
    80761 cttcgacctc aatagctatc agactgccag tatacgagtg tcaatgagta ttcaattaaa
    80821 cggcattaat tgcttctacg gcgcgcatca ggcgctgttc gatatcacgc tggattgccc
    80881 acagggcgaa acgctggtgt tacttggccc cagcggcgcg ggtaaaagct cgctgctgcg
    80941 tgtactcaat ctgcttgaga tgccgcgctc cggtacgctc aacattgcag gcaaccattt
    81001 cgatttcacc aaaacaccct ctgacaaagc gattcgcgat ttgcgtcgta acgttggcat
    81061 ggtgtttcag caatacaacc tgtggccgca tctgaccgtg cagcaaaacc tgattgaagc
    81121 gccctgccgt gtactggggt tgagtaaaga tcaggcgctg gcccgtgcag aaaaactgct
    81181 ggaacgtctg cgtctcaaac cttatagcga tcgttacccg ctgcatcttt ctggtggtca
    81241 gcagcagcgt gttgctattg cccgtgcgtt gatgatggaa ccgcaggtac tgctgttcga
    81301 tgaaccgacc gccgcactgg acccggaaat tacggcacaa atcgtcagca tcattcgtga
    81361 gctggcagaa acgaatatta cccaggtgat cgtcacccac gaagttgaag tggcgcgtaa
    81421 aaccgccagc cgagtggtgt atatggaaaa tggtcatatc gtagaacaag gcgacgcgag
    81481 ctgctttacc gagccgcaaa ccgaagcatt taaaaactat ctctctcact aagattcggg
    81541 ataacaacaa tgaaaaaagt tctgattgcc gcgttaattg caggttttag tctttccgcc
    81601 acagctgccg aaaccattcg ttttgctacc gaagcatcct atcctccgtt tgaatcgatt
    81661 gatgcaaaca accagatcgt tggttttgac gtcgacctgg cacaagcgct gtgtaaagag
    81721 attgatgcaa cctgcacttt ctctaaccag gcgtttgaca gcctgatccc aagcctgaaa
    81781 ttccgtcgcg tagaagccgt gatggcgggc atggatatca ctccggagcg tgaaaagcag
    81841 gtgctgttta ccaccccgta ctatgacaac tctgccctgt ttgtgggtca gcaaggcaaa
    81901 tacaccagtg ttgatcagct gaaaggcaaa aaagtcggcg tacagaacgg gacgacacac
    81961 cagaaattca ttatggataa gcacccggaa atcactaccg ttccgtatga cagctaccag
    82021 aacgcaaaac tggatctgca aaacgggcgt atcgacggcg tcttcggtga caccgcagtg
    82081 gtcactgagt ggctgaaaga taacccgaaa ctggcggcgg tgggcgacaa agtgaccgat
    82141 aaagattact tcggcactgg cctcggcatc gcggtacgtc agggcaacac tgagctgcag
    82201 cagaaactca acactgcgct ggaaaaagtg aagaaagatg gcacttacga aaccatctac
    82261 aacaaatggt tccagaagta attcctgatg aatgaatttt ttcctttagc aagcgccgcc
    82321 gggatgaccg tcggccttgc cgtttgtgca ttgattgtcg ggctggcgct ggcgatgttc
    82381 tttgcggtat gggagtcggc aaaatggcgt cctgtcgcgt gggcaggttc agcgctggta
    82441 accattctgc gtggcctgcc agaaattctg gtggtgctgt ttatctattt tggctcctcg
    82501 cagctgctgc tgacgctttc ggatggcttc actatcaatc ttgggttcgt gcagatccca
    82561 gtgcagatgg acattgagaa cttcgacgtt agcccgttcc tttgtggtgt catcgctctg
    82621 tcactgctgt atgccgccta tgcctcgcaa acgcttcggg gcgcgttgaa agcggtgccg
    82681 gtgggtcagt gggagtctgg tcaggcgctg gggctgtcga aatcggctat ctttttccgt
    82741 ctggtgatgc cgcagatgtg gcgtcatgcg ctgcctggcc tcggtaacca gtggctggtg
    82801 ctgctgaaag ataccgcgct ggtcagtttg attagtgtga atgatttaat gctgcaaaca
    82861 aaaagcatcg ctactcgtac ccaggaacca tttacctggt acattgtggc ggcggcgatt
    82921 tacctggtga tcaccctgct cagtcagtac attctcaaac gcattgacct gcgcgcgaca
    82981 cgttttgagc ggaggcccag ctaatgtttg agtatttacc cgaactgatg aaagggctac
    83041 acaccagcct gacgctaacc gttgcctcgc tgattgtggc actgattctg gcattgattt
    83101 ttaccatcat cctgacgctg aaaacgccgg tgctggtgtg gctggtgcgg ggttatatca
    83161 cgctgtttac cggtacgccg ctgctggtgc agatcttcct gatttattac gggccgggcc
    83221 agtttccgac tttgcaggag tatccggcac tgtggcattt gttgtcagaa ccgtggttat
    83281 gtgcgctgat tgcgttatcg ctgaacagtg cggcgtatac cacgcagctg ttttacggtg
    83341 caattcgtgc gatcccagaa ggtcagtggc agtcctgtag cgccctggga atgagcaaaa
    83401 aagatacgct ggcgatcctg ctgccgtatg cctttaaacg ctcgctctct tcttattcca
    83461 acgaagtggt gctggtgttc aaaagtacct ctctggctta caccattacg ctgatggaag
    83521 tgatgggata cagccagttg ttgtacggac gcacctacga tgtaatggtg ttcggtgcgg
    83581 cagggattat ttacctggtc gtcaacggcc tgctgacgct gatgatgcgt ctgatcgagc
    83641 gcaaagcact ggcattcgaa cggcgaaatt aatgtagctg cgtacattcg ccgggggggt
    83701 ttgtaggtct gataagacgc gccagcgtcg catcagacat cagcacggtg cctgtgccgg
    83761 atgcggcgtg gacgccttat ccggcctaca tagtgcataa attcttataa tgaagacgga
    83821 caactcacta aggtatccgt ctttttttat ttcatttaaa ttatttaatc atatttattg
    83881 catataaatt cacttgatgg cattgttatc ccatgccgca gacacggcca aaaatcataa
    83941 gattgacaga cgggagttcc atcatgaaaa agttagttct tgccgcttta cttgcttcct
    84001 ttactttcgg tgcttctgcc gcagagaaaa tcaattttgg cgtttcagcc acctatccac
    84061 cctttgaatc tataggtgct aataatgaga ttgtcggctt tgatatcgat ctggcaaaag
    84121 ccttgtgcaa acaaatgcag gcagaatgta cttttactaa tcacgcgttc gacagcctga
    84181 tcccgtcact gaaattcaga aaatatgacg ccgtaatctc cggtatggat atcacgcccg
    84241 agcgtagcaa gcaggtatcg tttaccacgc cctactatga aaactcagcc gtcgtgattg
    84301 ccaaaaaaga tacctacaaa acgtttgccg atctgaaagg caaacgtatt gggatggaaa
    84361 acggcaccac gcaccagaaa tatattcagg atcagcaccc cgaagtgaaa actgtctcct
    84421 atgacagtta tcagaatgcc tttatcgatc tgaaaaatgg tcgtattgat ggggtatttg
    84481 gtgacacagc ggtggtaaac gaatggctga aaaccaatcc gcaactgggc gttgctactg
    84541 aaaaagtgac cgatccgcaa tacttcggca ccggtctggg catcgctgta cgtccggata
    84601 acaaagctct gctggaaaaa ctgaataacg cgctggcagc aattaaagct gacggtacat
    84661 atcagaaaat caatgaccag tggtttccgc agtaattgag actgatgaca aaagtaagat
    84721 tgcctgatgc gctgcgctta tccggcccac atcaactctg caatgtattg atttagcatg
    84781 attttgtagg ccggataagg cgtacacgcc gcatccggca tgaacaaagc gcacctgatc
    84841 agcaatctga agccgcacct gcggcttttt tattgcttca ccagcagcgt cagcacttca
    84901 tagtgcgcgg tgtgcgggaa catatcaaaa agctgtaccc gttcgatgcg gtaacctggc
    84961 agttcgcgga tatctttcgc catggtttgg gcgttacagc tggagtagat aataaaacgc
    85021 ggtgccattg ttgagagata atcacatagc ggtttaccaa tgccgcggcg cggcggatta
    85081 accagcacca gctccggcac ctccccctga gcggtggcaa actgagtgga gtccagcgcc
    85141 tgaaattgca aacgcgttaa gcccagttca gcggctgact gctttgcaca ggcaatggcc
    85201 tctgatgcaa tttcgatccc ggttaactgc atgtcaggcg tcgcgcagtg taaaccaaag
    85261 ccacccacac cgcagaacag atcccacata tgtttaactg gcagctgccg tacccagtcg
    85321 cgcgcggtag cgtacagctg gctggcgacc gccggattag tctggaagaa actttgcgga
    85381 cggatccaca gcggtacgtc attaaaacgc tctgccagcg cctgttgttc agtaaggtag
    85441 atctccgtct ccccttccat aatcgccata tgtaccggct gaatattgac ggtaataact
    85501 ttcagttgcg gtagttgttc ctgtaaccac ggcagcgcct tacgcagttg tgccagtttg
    85561 gtttcagaac gcagtacaaa gcgcagcatc atgccgccat cgctctggct ttcagtcagc
    85621 agaatgtatt tcagttcgcc acgcttacgc gccacgttgt agggtgtcag ccccgcacgg
    85681 gcgataaacg gttttagcgc cgcaaaaacg ggcgcaaatg aggcaggata aagcgggcag
    85741 tcacaaaggt cttctggtgt gccgtcgcga tgcagcatac cgagcagtgg tttttcaacg
    85801 ctaccactca ccaccatttt ggctttatta cgaaaccctt gttccgggcc tgacaccggc
    85861 gcgcaccatt cctcaaccgg aaaatcggcg agcagatttt taagatcggc ggttttagcg
    85921 gagagttgct ctggaatcgg ctgcgttatc cactgacagg aacgacagcg acccgcgtcg
    85981 taaagtgcgc actgcataca ttgaccttca catcatcagg ggcgacgatt atacacatta
    86041 ttgcaactgg aagaaccgcc gactggtcga gggaacgaac agcagcatca ggatgagcat
    86101 atccggcagc ttctgcagca tcaggctatg gaagatttca cgttttgatt caccgggaat
    86161 gctgaacagc tccggataac cgtaccccag cgaagccgcc cacaagtaac ttgcggcggt
    86221 gatttgtgtc agcagatata gccagcgcgc ccagcgacgc cctttcacca gtgaaaacgc
    86281 acaccagatc tcaatgaaaa ccagcaccag actgcttaaa aagaccagcg ttaaactcca
    86341 ggtttgtacg ctgcgatgaa tgaactcgcc aataccgcgc acgcccagcg tattgaaaat
    86401 catcagcacg tcgaggccac ggatcataat aatggcgagc gccgccacct gcaccagcgc
    86461 aggcacattc aggcgagcat gagatgaaga tgttttctta aaaaatccca acgtttcgtc
    86521 ttccatgaaa acaatgccgc ggcatgcgcg gcattatgta gccaggttgg caaattttag
    86581 tgtcttcagc catgtcttgc acgctggata tcgcgtatcc gctgtttttc tgcgcgagcc
    86641 atcagatacc aggcgataaa tccgacaatt ccgaccgcac cgagaattaa cgttgcaagg
    86701 gcgttgattt ccggattcac ccccatccgc acgctggaaa agaccagcat cggtaacgtg
    86761 gtggctcccg gcccggaaac aaagctggcg atcaccagat catcaagcga caaagtaaaa
    86821 gccagtaacc agccagaaat gatcgcgggc atgatcatcg gtagcgtaat gacgaaaaac
    86881 actttcagcg gcgtcgcacc gagatccatc gctgcttctt ctatcgagcg atccagttcc
    86941 cgcaatcttg aagaaataac gaccgccaca taagccgtac aaaaagtgac atgcgccagc
    87001 cagatggtga gcataccgcg gtccgcaggc cagccaatgg catgagcaag cgcgacgaat
    87061 aataacaaca gcgacaagcc cgtgatgaca tctggcatca ccagcggcgc ggtgatcata
    87121 aaggcaaagc catttgatcc gcgaaacctg ccaaagcgca ccagcaccac cgccgcaata
    87181 gtcccgagga tcgccgccgc cgttgccgca caggcagcaa ttgtcaggct taaaccaacc
    87241 gcactcatca tcgcgtcatc gcgcaataac tcaccatacc agcgcgttga ccagccggcc
    87301 cacaccgtca ccagcttcga gctgttaaac gaatagataa ccagcatcag cattggcgcg
    87361 tagagaaagg taaagcccag cagcaaaatc acaatccgcc agggcgaacg aactaccggt
    87421 aaattattca tccgtgttct cccacgcttt tttgctggtg tttgtggaac cacattatcg
    87481 gcacaattag cagcagcaac atgatgatcg ctaccgccga ggccaccggc cagtcgcggt
    87541 tattgaaaaa ctcttgccat agcacgcgcc cgatcatgat gctgtccggg ccaccgagca
    87601 gttccgggat cacaaactcg cccacagccg ggataaacac cagcatcgat ccggcaataa
    87661 tcccaccttt agtcagcggc acgatcacag taaagaacgt tttcagcggt cgtgcaccga
    87721 gatccagcgc tgcttccacc agcgaataat caatacgaat caacgcggta taaatcggca
    87781 gtaccataaa cggcacgtag gcgtaaacaa tgccaatata aactgccaga ttggtatgaa
    87841 gaatggtcag cggttgatcg ataaccccca gccacagcag aaaattattc agcacaccgt
    87901 tgttttttaa tattcccatc caggcataaa cgcggatcag aaacgaggtc cacgacggca
    87961 ggatcaccag tagtaataaa atattacggg tcgaaggctt actgtgcgcc accgcccacg
    88021 ccagcggata gccgatcagt aaacagcaaa atgtcgaaat cgcagccacc tgtagcgact
    88081 ggagataagc atcgaaatag agcggatcgt cggtcagttg cagaaaatta ccgagattaa
    88141 gagtgatgga cagttgcccg tcagcccact ccatcagttc ggtataaggt ggaatagcgc
    88201 gcgccatctc cgccaggctt attttaaaga caatcagaaa tggcagcaga aacagcaaga
    88261 tcaaccagat atatggcaac gcaatgacca gtttgcgccc atgcttcatt tgcagctgcg
    88321 acaaccacag cttaaatccg cccggcggtt tcgactgggc agcaggttca agtgtattca
    88381 tctttgctcc ttaaaccgtc agcaccacac agctgtccac ttcccagcac aaacgcactt
    88441 cgtcgcccca ggtcggtaac cctttacgat gacgatgggc gttttgtagc tgggcgctaa
    88501 tcatctgccc acttttcaga cgaacgtgat acaccgaaag atcgccgaga taggcaatgt
    88561 gtatcacctc ccccaccgcg aagttacaac cattggcggg cggctcttcg caaagcatga
    88621 ttttttccgg gcgcagcgct acgtgtaccg gcacgttatc gaccactgaa gcatccgcat
    88681 cgactttcag tggatgcacc agccccggcg aatcaagcac caggccatct tcctgacgct
    88741 ctttgagtac accttcaaag acattaaccg aaccgataaa ttcagcgctg tagcgggtag
    88801 ttggatgctc gtagatctct tccggctcgc caatctggac aaatttcccg cgattcataa
    88861 tggcgatacg ccccgccatg gtcatcgcct cttcctgatc gtgggtgacc atcacacaag
    88921 tcacaccgac gcgctccaga atatccacca cttcaagctg catccggtca cgcagctttt
    88981 tatccagcgc gcccatcggc tcatcgagca gtaatagttt cgggcgtttg gcaaggcttc
    89041 gggccagggc cacacgctgt cgctgaccac cggaaagctg atgcggtttg cgtttggcga
    89101 actcctgcat gtgcaccagc ccaagcatct cgttgacccg ggtggcaatt tccgctttcg
    89161 gtagtttgtc ctgtttcagg ccaaaggcga tgttctgctc cacagtcata tggggaaaca
    89221 gcgcgtaaga ctgaaacatc atattgatgg ggcgcaggta aggcggcacc tgtgacaaat
    89281 cgacgccatc aagcattatc tgtccggcag aaggttgttc gaaacctgcc agcatacgca
    89341 gcagcgtgga cttgccacag ccggatgcgc ccagcagcgc gaagatttca cctttgtaga
    89401 tggtcaggct gacatcatcc accgcatgtt gaccatcgta ggatttggtg aggttgcgga
    89461 tttctaatag cggcgtcagc gccttacggg ttttcgcctg cgggcgaggg atagcgtcat
    89521 tcacggctgc tctccggcat tgaatgtgtg caagcgcacc atgatggtgc gaattgccgg
    89581 cgggtgtggt gcgcccgccc ggcatctaag gctgcggatt attttccgct cttcactttg
    89641 gtccacgcgc gggtgcgcac acggtcgatt ttcgggtcct gcactttcag agtgaacagc
    89701 ttcgcacgaa catccgcagg cggataaata cccgggttct cacggacttc cgcactcacc
    89761 agcggcgtgg ctgctttatt ggcgttggca tagaacacat tgtcggaaat atgcgctact
    89821 acatccgggc gcagcaggta attcaggaac tgataggctt cgtctttgtt tttggcatcc
    89881 gcaggcatgg cgaatacatc aaagaacgcc atcgcccctt cttttggaat cgagaacgag
    89941 acattcactc cattcttcgc ttccttcgcg cggtttgacg cctgccagac atcacctgcc
    90001 cagccgatag cgacgcaaat atcgccgttt gccaggtcgt taatgtattg agatgaatgg
    90061 aaataacgaa tgttcgggcg cagctttaac agcagatcgg ttgccggtcc ggtgtaatca
    90121 tccgctttag tgctgttggg atctttgccg agataattca acacggtagc aaaaacttct
    90181 tctggcgcat ccaggaaaga gacaccgcag cttttcagtt tttccagatt ttcaggtttg
    90241 aggatcaagt cccagctatc gacgggcgcg ttttcgccca gcacagcttt aactttatca
    90301 acgttatagc caatcccggt cgtcgcccac atatagggca tagcaaattt attgtcggga
    90361 tcgtgtttgg cgaccagctt cagcagctcc gggtcgagat tcttccactc cggcaatttg
    90421 cttttgtcca gcggctggaa aactcccgca gtcaactggc gttcaagaaa gctggcagat
    90481 ggaaccacca agtcaaagcc ggtgctaccc gccattaatt tgccttccag tacttcgtta
    90541 gagtcgaaaa catcgtagac gactttaata ccggtttctt tttcaaaatt ggccaccgtg
    90601 tccggggcga tataatcaga ccagttataa acgtggagtg ttttttgttc agccgcgagc
    90661 gtgccgacag agacggccat cagagcaccc gcagccagac ccgaaagcca ttttttattt
    90721 aaggcggtca tatctctttc cttctgaaag ttcgttaaca aacgaacaaa agttgtcaca
    90781 ctaaaagata tgcaataaac gtgcatattt tttatcggcg gggtgtaaga aaagagaact
    90841 ctctcccggc cagcactgct cgctatataa aagcatcgca agaataactt tagccagggt
    90901 ttgagactat agctcggatt aaaaaacggt atttaacaaa tttttatgcg tatttgctct
    90961 atgtgataaa gccaaatcga cacaagagaa gtgaaataat ctttaagaaa aggttaaaaa
    91021 cagcagataa ttttgcagcg cgcagccact gcaccagcga ctgcgtgctc gaaaggagtt
    91081 atcagtggag gaaatagttt tcggtaacgt tgttttgccc ttcatcgtca gtcggtaaca
    91141 gcagttgatg ggcattagct tcaagaatga ccatcgaaat ctgctcttcg ctctgacgca
    91201 cgaaccatgc aaactgctca taggttacgc cctgcataac ggataaagac tggcaaacca
    91261 ccagctttgg cagattatca tcctgcatat caagaaatgc tttcacggtc agcgaactgg
    91321 cattgatggc agataaatcc gccgccagcg gcaatacggc tgaaggtcgg acttccgcca
    91381 tggcagaaaa caggatcgtg ttatcgatca gatcgatttt ggcatcaaag acaccgtcga
    91441 aattctgcat atggggcaga tgcagagcct gacagttatc acattcaaaa aaactcatcc
    91501 ccaggtcatc gagccattga cgcagcgtat ccagaccagg aacgaccagc gatgtcataa
    91561 ttttgtacaa cctctttcga taaaaagacc ggcacagctt acgcaaaaag cgcaggcaaa
    91621 accatgatca gtaatgtgat tgcgactaac cacccgtttt caggcaatat tctgtcgtag
    91681 cgtggcgttc gatccagcgg atcattttac ccgcgatgtc gataccggtg gttttttcta
    91741 ttccttccag ccccggcgac gcattcacct ccatcaccaa cggcccgcga ttagcacgca
    91801 gaatatccac accagcaacg tccagcgcca tcgttcgcgc ggcttttatc gcgatttcac
    91861 gctcctgtgg tgtgatactg gcgacgcttg ccgcgccgcc acgatgcaaa ttggaacgaa
    91921 aatcgccctc tttcgcccgc cgttcaatcg cagcgaccac ttcatcgcca acaaccagac
    91981 agcggatatc gcacccttgc gcctctttga tatattcctg caccagaata tgcgcgttca
    92041 gaccgcggaa agcgtcaatc acgctttccg ccgcctgacg cgtctccgcc agcacgacgc
    92101 caattccctg cgtgccttca accaacttga ccaccagcgg cgcaccaccg accatgtcga
    92161 ttaaatcgct ggtatcatcc ggcgaatgcg caatgcccgt gacaggcagg tcgatgccct
    92221 gacgcgccag cagttgcatg gaacgcaatt tgtcacgcgc ccgggcaatg gcgaccgact
    92281 cattgagcgg atagctcccc agcatctcga actggcgcag tgccgccgtc ccataaaagg
    92341 taatggcggt gccaatacgc gggatcactg cgtcaaaatg gggtaacttg cggcctttgt
    92401 agtgaataga agacgccgca ggatttatgt tcatgtagca agaaagcgga tcaagaattt
    92461 caaccaggtg accgcgctgt atagcggctt cacgcagccg cttacacgaa tagagcgttc
    92521 catcccggga caatatggca attttcaccc tgcacctctc tgtcagacct ggtcaaaagc
    92581 ccgcgtatca tacatcgacg tggcggtttt agcgcgtcgc ccaaccctgt ttgtgcaaat
    92641 aatccagaat aaatgggcgg ctttctttaa tgattgttcg gcggatatga tcgctccagg
    92701 tatcccggcg attattgctg ccacgggtga ggtaatattc cgccagttgc tcgtcatact
    92761 gcgccagtgc gcctttatcc agcggttgat agctgttttc atgcaccaaa atggaggccg
    92821 gtaaacgcgg cttaagatcc ggattatccg caggccagcc aaggcacagc ccaaacagcg
    92881 gcagaacatg ctgcggtaat ttaagcagtt tcgtcaccgc ttcaatatta ttgcgcaggc
    92941 cgccgatata taccccgccc aatcccagcg attccgctgc gattaatgca ttctgcgcca
    93001 tcattgccgt atcaacgaca ccgagcaaca gttgttccgc caggccgagc tgagcatccg
    93061 gacagatctg taaatggcgg ttaaagtcgg cacagaacac ccagaactcc gccgcttgcg
    93121 ctacgtgttt ttgcccgccg gtcagcgtca ccagttcttc acgtaacgct ttgtcggtaa
    93181 tgcgaataat gctactgcac tgcaaaaaac tggaactgga cgtcgcacgg gcgctgttaa
    93241 taatcgcctc acgctgcgct tcggaaatgg gttcatcagt gaaatggcga atggagcgat
    93301 ggccacaaat aagttcaatg gttggcgtca ttatcttttt ctctttctga acgtgaatat
    93361 tgcggtggac ggttcatcag ctgtggggca agacgttttg ccacctgaag aataaccacc
    93421 accgcagcgg gaagcatgag caaaacaccg agaaaaatca tcagaatctg cacttctggc
    93481 cgagaaaatg gctcaggcag cgacagggag tcgcttaccg acagcagcgc caccgccagt
    93541 agcatcattc cgataaattc cagtatcaac acgcctttag gcaatttacc gatcgcgcgc
    93601 atacgcttcc ctctgcaaag tgagccttca gtctaaaact tttcactgta ttgtgtttaa
    93661 cagttatagc ttttagcaat taatgcaaca ggttaaacct actttcagcg aatacatttt
    93721 agcgtgatca ttacaggcat aaatctatga ggagagaaat aatgcaaacc gttatttttg
    93781 gtcgttcggg ttgcccttac tgtgtgcgtg caaaagatct ggctgagaaa ttgagcaatg
    93841 aacgcgatga ttttcagtat cagtatgtag atattcgtgc ggaagggatc actaaagaag
    93901 atctacaaca aaaggcaggt aaacccgtag aaaccgtgcc gcagattttt gtcgatcagc
    93961 aacatatcgg cggctatacc gattttgctg catgggtgaa agaaaatctg gacgcctgat
    94021 cgtctgacaa gccctcgcgt tgagggcttt actgattttt tctgtgctgt ggtttaaaca
    94081 aactactgat aaataagaaa cacagtgccc ccagcgcaca ccagaacacc gcgcttagta
    94141 accatgccag ctcttgccag aatgagcgcg tcggtgaaaa aaacagccgc ataatgagca
    94201 ttgaacaggg tgccgccagc attgcgccaa atagaggttt caggacttct ctacgctgtg
    94261 aaaagaagct ggcgactgct ccaggaagaa tgaaaaatag caagccgatt tcaggatgcc
    94321 cggcagcccg aaaagcgcct ttcatgtgcg tcgccagaaa aaggcacacc acaatgaaga
    94381 ggacaaaaca gcagattgcc cccgcccaac gttgtttatg tttcactcgt tcctcctgac
    94441 actgcgtcta tcgaacacat ttttcgccag tgtggcgttc agtaagataa agccgcttcg
    94501 cattccatgc taatataggc caacgcaatt catatagccg ttgataccta atgtgattac
    94561 actagtaaaa tatattgtta ctttactatc gtttaggtgc gctgaatgaa tctgcgccct
    94621 gaattctggt aaaaaacatt atcgtaaatt accatttctt tcaacagctt actagtaaac
    94681 aagaagttag cctccgtgaa tataaacgtc gccgaattgt taaatgggaa ttacattctg
    94741 ttattatttg tggtcctcgc gcttgggcta tgtctcggaa agttacgact tggttcgatc
    94801 caactgggta attccattgg cgttttagtc gtatcgctgt tattaggcca acaacatttc
    94861 agcattaaca ccgatgcgct taatcttggc tttatgctgt ttattttctg cgtcggggtc
    94921 gaagccggac cgaacttttt ttccattttt tttcgcgatg ggaaaaatta cctaatgtta
    94981 gcactggtga tggttggcag tgcgctggtg atcgccttag ggttaggtaa gctgtttggc
    95041 tgggatattg gcctgacggc cggtatgtta gcaggctcta tgacgtcgac accggttctg
    95101 gtcggtgctg gcgatacact gcgtcattcc ggcatggaaa gcaggcagct ctcactggca
    95161 ctggataatc tgagcctcgg gtatgcctta acctatttaa tcggtctggt gagtttgatt
    95221 gttggtgcgc gttacttgcc gaaattgcag catcaggact tacagaccag cgcccagcaa
    95281 atcgcccgcg aacgtggcct ggacactgat gccaaccgta aggtttattt accggtgatc
    95341 cgcgcctatc gcgtcggccc ggagctggtg gcctggaccg acggcaaaaa tctgcgtgaa
    95401 ctgggtattt atcgacaaac cggctgctac attgaacgta ttcgacgtaa cgggattctg
    95461 gcaaatccag acggtgatgc cgtgctacaa atgggcgatg aaatagcgtt ggtaggctat
    95521 cccgacgccc atgcccgact cgatcccagc ttccgtaacg gtaaagaagt tttcgatcgt
    95581 gaccttctcg acatgcgtat cgtcactgaa gaagtggtcg ttaaaaacca taacgctgta
    95641 ggtaaacgtc tcgcacaact gaagttgacc gatcacggtt gcttccttaa ccgcgtcatt
    95701 cgtagccaga ttgagatgcc gatagatgac aacgtcgtgc ttaacaaagg tgacgtttta
    95761 caagtcagcg gtgatgcccg tcgcgtaaaa accatcgccg atcgcatcgg ctttatctcg
    95821 attcacagcc aggtcactga cctgctggca ttttgcgcct tctttgttat tgggctgatg
    95881 atcgggatga tcaccttcca gttcagcaca ttcagtttcg gcatggggaa cgctgccggg
    95941 ttgttattcg ccggaattat gctgggcttt atgcgtgcta accacccgac cttcggttac
    96001 attccgcaag gtgcattaag catggtgaaa gagttcggct tgatggtgtt tatggcaggc
    96061 gttggtctga gcgccggtag cggtattaat aacggcctgg gcgcgattgg cggtcagatg
    96121 ttgattgccg gattaattgt cagtcttgtg cccgtggtta tctgtttctt gttcggtgct
    96181 tatgtattgc gaatgaaccg cgcactgttg ttcggcgcaa tgatgggcgc acgcacctgc
    96241 gcgccggcaa tggagatcat cagtgataca gctcgcagta acatccctgc gctgggctat
    96301 gcgggcacct acgcaatcgc caacgtcctg ctgacgctgg cagggacaat catcgtcatg
    96361 gtatggccag gattaggata aaactgaagt tgccctgaaa atgaaatttt tttgcacaac
    96421 cgcagaactt ttccgcaggg catcagtctt aattagtgcc actgcttttc tttgatgtcc
    96481 ccattttgtg gagcccatca accccgccat ttcggttcaa ggttgatggg ttttttgtta
    96541 tctaaaattt atctactacc ctgcaaccct ctcaaccatc ctcaaaatct cctcgcgcga
    96601 tagcggctta cggtcggtga caaaatgcag cgtcatcccc tcaataaacg catcaagcgc
    96661 gcgggcggtt ccgggttcaa accattgttc gagcgtttgc tgactgcgct gcatccagtt
    96721 ttgcattacc gtttttaata gcggttttcg gctagccagc gcgtagagct ggtacatcag
    96781 ctccatgtta tccggcgttg caacctgtga gctgtagatc atatcggtga tagcctggca
    96841 tgcgccttga gcatcactaa catcgctaaa aaatgcctga tattgccggg acataatctc
    96901 agtaaaactg ctgaacgcct ccagcaacaa ctcatcaatt cctgaaaaat agtaggtcat
    96961 cgatcccaac ggtaccccgg caagggtagc aattttgcgg tgcgtaacag catgtattcc
    97021 gtaaagtttc accgcctcca gcgtggcctg gataattttt tctcgccgtt gcggatcgtt
    97081 agcgcgacgc atgtcatttc ctctgtctgc aattgtgtac aaatgtacac aaccttgcta
    97141 atgttgtctt ccctcttctt attttgacgt gatctatgac cgtaaattct tcacgtaatg
    97201 cattgaaacg ccgaacctgg gcgctgttta tgttcttctt tttgccgggc ctgttaatgg
    97261 cgtcctgggc aacccgtacg cctgctatcc gcgatattct ctctgtctcg atcgctgaaa
    97321 tgggcggtgt tctctttggt ctgtcgatcg gttcgatgag cggtattctc tgctcggcgt
    97381 ggttagtgaa acgctttggg acacgtaatg tcatcctggt cacgatgtcc tgcgcattga
    97441 tcgggatgat gatattaagt ctggcactct ggctgacatc acccctgctc ttcgcggttg
    97501 gcctcggcgt ctttggggca agttttggtt ctgcggaagt ggcgataaac gtcgaaggtg
    97561 ccgccgttga gcgagaaatg aataaaacgg ttttgccgat gatgcacggt ttttatagcc
    97621 tgggcacgct ggcaggcgct ggtgtcggga tggcactgac ggcctttggc gttccggcaa
    97681 cggtgcacat tttattggcg gcgctggtag gaatcgcgcc tatttatatc gccattcagg
    97741 caatccctga cggtacgggc aaaaatgctg ccgatggcac ccagcatggt gaaaaaggcg
    97801 taccttttta tcgcgatatc cagttgctgc tgattggtgt tgtggtgctg gcgatggcct
    97861 ttgccgaagg ttctgccaac gactggttac ccttattaat ggttgacggt cacggtttta
    97921 gccctacttc cggctcgctg atttatgccg gttttaccct ggggatgact gttggacgct
    97981 ttaccggcgg ttggttcatc gaccgttaca gtcgcgttgc cgtggttcgg gccagtgcac
    98041 taatgggagc gttgggtatt gggctgatta tttttgtcga tagcgcctgg gtcgctgggg
    98101 tgtctgttgt actctgggga ctgggtgcct cgctgggctt cccgctgacc atttctgccg
    98161 ccagcgatac cggccccgat gcaccaaccc gcgtcagtgt ggtagcaacg accggttatc
    98221 tggctttcct cgtcgggccg ccgctgctgg gctatctcgg cgaacattat ggattacgta
    98281 gtgcaatgct ggttgtactg gcgctggtta ttctcgcggc tattgtcgcg aaagccgtcg
    98341 ccaaacccga taccaaaacg cagacggcga tggagaatag ttgatgagca ttaaattaat
    98401 tgcggtagac atggatggta ctttcttaag cgatcaaaaa acctataacc gtgagcggtt
    98461 tatggctc
//
LOCUS       DAZGOB010000002        84605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGOB010000002 DAZGOB010000000
VERSION     DAZGOB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.002"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..76)
                     /locus_tag="ACHLIF_000086"
     CDS             complement(<1..76)
                     /locus_tag="ACHLIF_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9604166.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKK"
     gene            94..285
                     /gene="yfgG"
                     /locus_tag="ACHLIF_000087"
     CDS             94..285
                     /gene="yfgG"
                     /locus_tag="ACHLIF_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO9604167.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            596..1114
                     /gene="yfgH"
                     /locus_tag="ACHLIF_000088"
     CDS             596..1114
                     /gene="yfgH"
                     /locus_tag="ACHLIF_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO9604168.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            1130..1669
                     /gene="yfgI"
                     /locus_tag="ACHLIF_000089"
     CDS             1130..1669
                     /gene="yfgI"
                     /locus_tag="ACHLIF_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO9604169.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKRVGKAEENKSDSWFN"
     gene            complement(1764..3341)
                     /gene="guaA"
                     /locus_tag="ACHLIF_000090"
     CDS             complement(1764..3341)
                     /gene="guaA"
                     /locus_tag="ACHLIF_000090"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO9604170.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(3410..4876)
                     /gene="guaB"
                     /locus_tag="ACHLIF_000091"
     CDS             complement(3410..4876)
                     /gene="guaB"
                     /locus_tag="ACHLIF_000091"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO9604171.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            5038..6408
                     /gene="xseA"
                     /locus_tag="ACHLIF_000092"
     CDS             5038..6408
                     /gene="xseA"
                     /locus_tag="ACHLIF_000092"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO9604172.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(6405..6620)
                     /gene="yfgJ"
                     /locus_tag="ACHLIF_000093"
     CDS             complement(6405..6620)
                     /gene="yfgJ"
                     /locus_tag="ACHLIF_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9604173.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6689..8161)
                     /gene="der"
                     /locus_tag="ACHLIF_000094"
     CDS             complement(6689..8161)
                     /gene="der"
                     /locus_tag="ACHLIF_000094"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO9604174.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(8279..9457)
                     /gene="bamB"
                     /locus_tag="ACHLIF_000095"
     CDS             complement(8279..9457)
                     /gene="bamB"
                     /locus_tag="ACHLIF_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO9604175.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(9468..10088)
                     /gene="yfgM"
                     /locus_tag="ACHLIF_000096"
     CDS             complement(9468..10088)
                     /gene="yfgM"
                     /locus_tag="ACHLIF_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO9604176.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(10106..11380)
                     /gene="hisS"
                     /locus_tag="ACHLIF_000097"
     CDS             complement(10106..11380)
                     /gene="hisS"
                     /locus_tag="ACHLIF_000097"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO9604177.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(11491..12609)
                     /gene="ispG"
                     /locus_tag="ACHLIF_000098"
                     /gene_synonym="gcpE"
     CDS             complement(11491..12609)
                     /gene="ispG"
                     /locus_tag="ACHLIF_000098"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO9604178.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12636..13649)
                     /gene="rodZ"
                     /locus_tag="ACHLIF_000099"
     CDS             complement(12636..13649)
                     /gene="rodZ"
                     /locus_tag="ACHLIF_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO9604179.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13934..15088)
                     /gene="rlmN"
                     /locus_tag="ACHLIF_000100"
     CDS             complement(13934..15088)
                     /gene="rlmN"
                     /locus_tag="ACHLIF_000100"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO9604180.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(15238..15669)
                     /gene="ndk"
                     /locus_tag="ACHLIF_000101"
     CDS             complement(15238..15669)
                     /gene="ndk"
                     /locus_tag="ACHLIF_000101"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO9604181.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15818..18130)
                     /gene="pbpC"
                     /locus_tag="ACHLIF_000102"
     CDS             complement(15818..18130)
                     /gene="pbpC"
                     /locus_tag="ACHLIF_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO9604182.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NANRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(18131..23092)
                     /gene="yfhM"
                     /locus_tag="ACHLIF_000103"
     CDS             complement(18131..23092)
                     /gene="yfhM"
                     /locus_tag="ACHLIF_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO9604183.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPSETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNHHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANDVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            23299..24144
                     /gene="sseA"
                     /locus_tag="ACHLIF_000104"
     CDS             23299..24144
                     /gene="sseA"
                     /locus_tag="ACHLIF_000104"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO9604184.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTEQVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            24480..24605
                     /gene="timP"
                     /locus_tag="ACHLIF_000105"
     CDS             24480..24605
                     /gene="timP"
                     /locus_tag="ACHLIF_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO9604185.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24637..25413)
                     /gene="sseB"
                     /locus_tag="ACHLIF_000106"
     CDS             complement(24637..25413)
                     /gene="sseB"
                     /locus_tag="ACHLIF_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO9604186.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPGFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25555..26838)
                     /gene="pepB"
                     /locus_tag="ACHLIF_000107"
     CDS             complement(25555..26838)
                     /gene="pepB"
                     /locus_tag="ACHLIF_000107"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO9604187.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(27016..27216)
                     /gene="iscX"
                     /locus_tag="ACHLIF_000108"
     CDS             complement(27016..27216)
                     /gene="iscX"
                     /locus_tag="ACHLIF_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO9604188.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(27228..27563)
                     /gene="fdx"
                     /locus_tag="ACHLIF_000109"
     CDS             complement(27228..27563)
                     /gene="fdx"
                     /locus_tag="ACHLIF_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO9604189.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27565..29415)
                     /gene="hscA"
                     /locus_tag="ACHLIF_000110"
     CDS             complement(27565..29415)
                     /gene="hscA"
                     /locus_tag="ACHLIF_000110"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO9604190.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(29432..29947)
                     /gene="hscB"
                     /locus_tag="ACHLIF_000111"
     CDS             complement(29432..29947)
                     /gene="hscB"
                     /locus_tag="ACHLIF_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO9604191.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(30043..30366)
                     /gene="iscA"
                     /locus_tag="ACHLIF_000112"
     CDS             complement(30043..30366)
                     /gene="iscA"
                     /locus_tag="ACHLIF_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO9604192.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(30383..30769)
                     /gene="iscU"
                     /locus_tag="ACHLIF_000113"
     CDS             complement(30383..30769)
                     /gene="iscU"
                     /locus_tag="ACHLIF_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO9604193.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30797..32011)
                     /gene="iscS"
                     /locus_tag="ACHLIF_000114"
     CDS             complement(30797..32011)
                     /gene="iscS"
                     /locus_tag="ACHLIF_000114"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO9604194.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(32252..32740)
                     /gene="iscR"
                     /locus_tag="ACHLIF_000115"
     CDS             complement(32252..32740)
                     /gene="iscR"
                     /locus_tag="ACHLIF_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO9604195.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(33041..33778)
                     /gene="trmJ"
                     /locus_tag="ACHLIF_000116"
     CDS             complement(33041..33778)
                     /gene="trmJ"
                     /locus_tag="ACHLIF_000116"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO9604196.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAAQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33897..34700
                     /gene="suhB"
                     /locus_tag="ACHLIF_000117"
     CDS             33897..34700
                     /gene="suhB"
                     /locus_tag="ACHLIF_000117"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO9604197.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34845..35699
                     /gene="yfhR"
                     /locus_tag="ACHLIF_000118"
     CDS             34845..35699
                     /gene="yfhR"
                     /locus_tag="ACHLIF_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9604198.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35890..37170
                     /gene="csiE"
                     /locus_tag="ACHLIF_000119"
     CDS             35890..37170
                     /gene="csiE"
                     /locus_tag="ACHLIF_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO9604199.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(37162..38301)
                     /gene="hcaT"
                     /locus_tag="ACHLIF_000120"
     CDS             complement(37162..38301)
                     /gene="hcaT"
                     /locus_tag="ACHLIF_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO9604200.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(38461..39351)
                     /gene="hcaR"
                     /locus_tag="ACHLIF_000121"
     CDS             complement(38461..39351)
                     /gene="hcaR"
                     /locus_tag="ACHLIF_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO9604201.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            39487..40848
                     /gene="hcaE"
                     /locus_tag="ACHLIF_000122"
     CDS             39487..40848
                     /gene="hcaE"
                     /locus_tag="ACHLIF_000122"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO9604202.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40845..41363
                     /gene="hcaF"
                     /locus_tag="ACHLIF_000123"
     CDS             40845..41363
                     /gene="hcaF"
                     /locus_tag="ACHLIF_000123"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO9604203.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            41363..41683
                     /gene="hcaC"
                     /locus_tag="ACHLIF_000124"
     CDS             41363..41683
                     /gene="hcaC"
                     /locus_tag="ACHLIF_000124"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO9604204.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41680..42492
                     /gene="hcaB"
                     /locus_tag="ACHLIF_000125"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41680..42492
                     /gene="hcaB"
                     /locus_tag="ACHLIF_000125"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO9604205.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            42502..43704
                     /gene="hcaD"
                     /locus_tag="ACHLIF_000126"
     CDS             42502..43704
                     /gene="hcaD"
                     /locus_tag="ACHLIF_000126"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO9604206.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43801..44223
                     /gene="yphA"
                     /locus_tag="ACHLIF_000127"
     CDS             43801..44223
                     /gene="yphA"
                     /locus_tag="ACHLIF_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO9604207.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(44271..45143)
                     /gene="yphB"
                     /locus_tag="ACHLIF_000128"
     CDS             complement(44271..45143)
                     /gene="yphB"
                     /locus_tag="ACHLIF_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO9604208.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(45155..46249)
                     /gene="yphC"
                     /locus_tag="ACHLIF_000129"
     CDS             complement(45155..46249)
                     /gene="yphC"
                     /locus_tag="ACHLIF_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO9604209.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(46282..47280)
                     /gene="yphD"
                     /locus_tag="ACHLIF_000130"
     CDS             complement(46282..47280)
                     /gene="yphD"
                     /locus_tag="ACHLIF_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9604210.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(47305..48816)
                     /gene="yphE"
                     /locus_tag="ACHLIF_000131"
     CDS             complement(47305..48816)
                     /gene="yphE"
                     /locus_tag="ACHLIF_000131"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9604211.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIIAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(48839..49822)
                     /gene="yphF"
                     /locus_tag="ACHLIF_000132"
     CDS             complement(48839..49822)
                     /gene="yphF"
                     /locus_tag="ACHLIF_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO9604212.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49919..53200)
                     /gene="yphG"
                     /locus_tag="ACHLIF_000133"
     CDS             complement(49919..53200)
                     /gene="yphG"
                     /locus_tag="ACHLIF_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO9604213.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            53318..54511
                     /gene="yphH"
                     /locus_tag="ACHLIF_000134"
     CDS             53318..54511
                     /gene="yphH"
                     /locus_tag="ACHLIF_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO9604214.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54575..55828)
                     /gene="glyA"
                     /locus_tag="ACHLIF_000135"
     CDS             complement(54575..55828)
                     /gene="glyA"
                     /locus_tag="ACHLIF_000135"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO9604215.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            56156..57346
                     /gene="hmpA"
                     /locus_tag="ACHLIF_000136"
     CDS             56156..57346
                     /gene="hmpA"
                     /locus_tag="ACHLIF_000136"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO9604216.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(57391..57729)
                     /gene="glnB"
                     /locus_tag="ACHLIF_000137"
     CDS             complement(57391..57729)
                     /gene="glnB"
                     /locus_tag="ACHLIF_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO9604217.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57790..59124)
                     /gene="glrR"
                     /locus_tag="ACHLIF_000138"
     CDS             complement(57790..59124)
                     /gene="glrR"
                     /locus_tag="ACHLIF_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO9604218.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(59114..59827)
                     /gene="qseG"
                     /locus_tag="ACHLIF_000139"
     CDS             complement(59114..59827)
                     /gene="qseG"
                     /locus_tag="ACHLIF_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO9604219.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59992..61419)
                     /gene="qseE"
                     /locus_tag="ACHLIF_000140"
     CDS             complement(59992..61419)
                     /gene="qseE"
                     /locus_tag="ACHLIF_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO9604220.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCICRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61995..65882)
                     /gene="purL"
                     /locus_tag="ACHLIF_000141"
                     /gene_synonym="purI"
     CDS             complement(61995..65882)
                     /gene="purL"
                     /locus_tag="ACHLIF_000141"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO9604221.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGLGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            66140..67696
                     /gene="mltF"
                     /locus_tag="ACHLIF_000142"
     CDS             66140..67696
                     /gene="mltF"
                     /locus_tag="ACHLIF_000142"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO9604222.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67693..68196)
                     /gene="tadA"
                     /locus_tag="ACHLIF_000143"
     CDS             complement(67693..68196)
                     /gene="tadA"
                     /locus_tag="ACHLIF_000143"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO9604223.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(68254..68889)
                     /gene="yfhb"
                     /locus_tag="ACHLIF_000144"
     CDS             complement(68254..68889)
                     /gene="yfhb"
                     /locus_tag="ACHLIF_000144"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO9604224.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            69098..69946
                     /gene="yfhH"
                     /locus_tag="ACHLIF_000145"
     CDS             69098..69946
                     /gene="yfhH"
                     /locus_tag="ACHLIF_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO9604225.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            70002..70262
                     /gene="yfhL"
                     /locus_tag="ACHLIF_000146"
     CDS             70002..70262
                     /gene="yfhL"
                     /locus_tag="ACHLIF_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO9604226.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(70456..70536)
                     /gene="shoB"
                     /locus_tag="ACHLIF_000147"
     CDS             complement(70456..70536)
                     /gene="shoB"
                     /locus_tag="ACHLIF_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO9604227.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70956..71336)
                     /gene="acpS"
                     /locus_tag="ACHLIF_000148"
     CDS             complement(70956..71336)
                     /gene="acpS"
                     /locus_tag="ACHLIF_000148"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO9604228.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(71336..72067)
                     /gene="pdxJ"
                     /locus_tag="ACHLIF_000149"
     CDS             complement(71336..72067)
                     /gene="pdxJ"
                     /locus_tag="ACHLIF_000149"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO9604229.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(72079..72807)
                     /gene="recO"
                     /locus_tag="ACHLIF_000150"
     CDS             complement(72079..72807)
                     /gene="recO"
                     /locus_tag="ACHLIF_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO9604230.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72819..73724)
                     /gene="era"
                     /locus_tag="ACHLIF_000151"
     CDS             complement(72819..73724)
                     /gene="era"
                     /locus_tag="ACHLIF_000151"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO9604231.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(73721..74401)
                     /gene="rnc"
                     /locus_tag="ACHLIF_000152"
     CDS             complement(73721..74401)
                     /gene="rnc"
                     /locus_tag="ACHLIF_000152"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO9604232.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(74674..75648)
                     /gene="lepB"
                     /locus_tag="ACHLIF_000153"
     CDS             complement(74674..75648)
                     /gene="lepB"
                     /locus_tag="ACHLIF_000153"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO9604233.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(75664..77463)
                     /gene="lepA"
                     /locus_tag="ACHLIF_000154"
     CDS             complement(75664..77463)
                     /gene="lepA"
                     /locus_tag="ACHLIF_000154"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO9604234.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(77661..78140)
                     /gene="rseC"
                     /locus_tag="ACHLIF_000155"
     CDS             complement(77661..78140)
                     /gene="rseC"
                     /locus_tag="ACHLIF_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO9604235.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKVSCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(78137..79093)
                     /gene="rseB"
                     /locus_tag="ACHLIF_000156"
     CDS             complement(78137..79093)
                     /gene="rseB"
                     /locus_tag="ACHLIF_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO9604236.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(79093..79743)
                     /gene="rseA"
                     /locus_tag="ACHLIF_000157"
     CDS             complement(79093..79743)
                     /gene="rseA"
                     /locus_tag="ACHLIF_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO9604237.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79776..80351)
                     /gene="rpoE"
                     /locus_tag="ACHLIF_000158"
     CDS             complement(79776..80351)
                     /gene="rpoE"
                     /locus_tag="ACHLIF_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO9604238.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(80348..80503)
                     /gene="rseD"
                     /locus_tag="ACHLIF_000159"
     CDS             complement(80348..80503)
                     /gene="rseD"
                     /locus_tag="ACHLIF_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO9604239.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80759..82381
                     /gene="nadB"
                     /locus_tag="ACHLIF_000160"
     CDS             80759..82381
                     /gene="nadB"
                     /locus_tag="ACHLIF_000160"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO9604240.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(82366..83103)
                     /gene="trmN"
                     /locus_tag="ACHLIF_000161"
     CDS             complement(82366..83103)
                     /gene="trmN"
                     /locus_tag="ACHLIF_000161"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO9604241.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            83235..84569
                     /gene="srmB"
                     /locus_tag="ACHLIF_000162"
     CDS             83235..84569
                     /gene="srmB"
                     /locus_tag="ACHLIF_000162"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO9604242.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT        20133 a        23192 c        21255 g        20025 t
ORIGIN      
        1 cttttttaca taacgaagaa ttgaccattt tgtcctgttg tgccttaatg taagtaccgt
       61 ccacagcgtg ggacatactt caaggaacct tttgtgagtc aggcaaccag tatgcgaaaa
      121 cgacaccgat ttaacagtcg catgacccgt atcgtactgc tcatcagctt tatcttcttc
      181 tttggccgtt ttatctactc gtccgtcggt gcctggcagc accatcagag caaaaaagaa
      241 gctcagcaat ccacactctc cgtcgaatca ccggtacaac gttagcggtt accttctcca
      301 ctttcacaga acctaacggc acttcgctgt cggatgcttt tgctctttgg aattatcaaa
      361 gcggcagata ttctttcatc ttaaatttta cgtctttgtc ctgactgatg tttatcctgt
      421 ttggctgcga aataaatata aaattaatat atatgttgta atgatatatt tttataaatt
      481 attccctgcg tgaattttaa taaatttaat ctatcccttt atacgcaata catttacttt
      541 cctcttttga tgatcttaaa tgtcttattt ttcgtaatgt gtataacaag gaatagtgat
      601 gaaatttaaa aaatgtcttc tgcctgtggc aatgttagcg tcattcactc tggcaggatg
      661 ccagtcaaat gctgacgatc atgccgccga tgtttatcaa accgatcaac tgaataccaa
      721 acaagaaact aaaaccgtta atattatttc cattcttccc gcaaaagttg ccgtagacaa
      781 ctcccaaaat aaacggaacg cacaagcctt cggcgcgctt attggcgcag tcgctggcgg
      841 tgttatcggc cacaacgtcg gttctggcag caattccgga acgacggcag gggcagttgg
      901 cggcggagct gtaggcgcgg cagtgggttc tatggtgaat gataaaacct tagtggaagg
      961 tgtttcttta acatataagg aaggcaccaa agtgtatacc tctacccagg tgggtaaaga
     1021 gtgccagttt acgacaggtt tagccgttgt tattaccaca acgtataacg aaacgcgtat
     1081 tcagccaaat actaaatgtc ctgaaaagag ctaataatca ggaggagtca tgaagaaagt
     1141 ttttctttgc gccatcttgg cctccttaag ctatccggct atcgcctcat cattgcagga
     1201 tcaactctct gctgtagcag aagctgaaca gcaaggtaaa aatgaagagc aaaggcagca
     1261 tgacgaatgg gtcgcggagc gcaacaggga aatccagcaa gagaagcaac gtcgcgcaaa
     1321 tgcccaggcc gccgctaaca aaagagcggc aacggcagcg gcaaataaga aagctcgtca
     1381 ggataaactg gacgccgaag cctctgcgga caaaaaacgc gatcaaagtt atgaagatga
     1441 gctacgcagc ttagagattc agaaacaaaa actggcgctg gcaaaagaag aagcccgcgt
     1501 taagcgagaa aacgaattta tcgatcagga actgaagcac aaagctgcgc aaaccgatgt
     1561 ggtgcaatct gaagctgacg ccaacagaaa tatgactgaa ggcggtcgcg atctgatgaa
     1621 acgcgtgggc aaagcagaag agaacaaatc ggacagctgg tttaattaat cgatgttagt
     1681 aacttcaagc ctatgattct tgaagataaa aaaccctctg tagtaacaga gggttttgtt
     1741 cattcatagt gcagggatta aaatcattcc cactcaatgg tagctggcgg cttgccgctg
     1801 atgtcataca ccacgcggga aataccgttc acttcattga taatgcggtt ggaaacgcga
     1861 ccgaggaaat cgtacggcag atgcgcccag tgtgcggtca taaagtcgat ggtttcgaca
     1921 gcacgcagag agacaaccca atcatactta cgaccatcgc ccattacgcc aacggaacgt
     1981 accggcagga acacagtgaa cgcctggctg actttgtcgt acaggtccgc tttacgcagt
     2041 tcttcaatga agatggcgtc agcacggcgc agcaggtcac agtactcttt cttcacttca
     2101 cccagcacac gaacgccaag gcctggtccc gggaacgggt gacggtacag catgtcgtac
     2161 ggcaggccca gctccagacc aatcttacgc acttcgtctt tgaacagctc tttcagcggt
     2221 tcaaccaggc ccatcttcat ctctttcggc aggccgccca cgttgtggtg agatttgatg
     2281 acgtgtgctt taccggttgc agaagccgca gattcgataa cgtcagggta gatggtgccc
     2341 tgcgccagcc acttcacgtc ttccagtttc agcgcttctt catcgaatac ttcaacgaaa
     2401 acgcgcccga tgattttacg ttttgcttcc ggatcgtttt cgccagccag cgctgacagg
     2461 aagcgatctt ctgccggtac gtgaacaatg ttaagaccaa agtgatcgcc aaacatatcc
     2521 agaacctgct ctgcttcgtt gagacgcagc aggccgttgt cgacgaatac gcaagtcagg
     2581 tttttaccga tagcgcggtg cagcagcatt gcggttacgg aggaatccac accaccagag
     2641 aggccgagga tgactttatc gtcgcctacc tgctcgcgga tgcgagctac agcatcgtcg
     2701 ataattttcg ctggcgtcca cagggcttca cactggcaga tatcacgcac aaaacgctcc
     2761 agcatgcgca taccctggcg ggtgtgagtc acttccgggt ggaactgtac gccatagaag
     2821 cgtttttctt cgttagccat aatggcaaac gggcagcttt cggtgctggc tacggtgacg
     2881 aagtcggacg ggatagcggt aactttatcg ccgtggctca tccagacatc gagcagcggt
     2941 ttaccgtctg cggtcagcgc atcttcgata ccgcgaacca gtgcgctgtc gtttacgact
     3001 tcaacctgcg cgtagccaaa ttcacgttcg ttagaggctt caacgtgacc gcccaactgc
     3061 attgccatgg tctgcatgcc atagcaaacg ccgaataccg gtacgcctgc ttcaaagaca
     3121 tactgcggcg cacgcgggct gttttcttcg gtggtgcttt ccgggccgcc ggaaagaata
     3181 atgccgcttg gattgaagtc acgaatttgt gcttctgtca catcccacgc ccacagttcg
     3241 cagtaaacac ccagctcacg cacgcggcgc gcaaccagtt gagtgtactg agaaccgaag
     3301 tccagaatga ggatgcgatg cttatgaatg ttttccgtca ttgacgctta ttccgaggca
     3361 agtgaaacag ataatataaa tcgcccgaca tgaagtcggg cgaagagaat caggagccca
     3421 gacggtagtt cggggactct ttagtaatgg tcacgtcgtg aacgtggctt tcctgaatgc
     3481 ccgcaccgct gatacgtaca aactccgctt tagtacgcag ttcgtcgata gtaccacagc
     3541 cggtcagacc catacaggag cgcaggccgc ccatctgctg gtgaatgatc tctttcaggc
     3601 gacctttata agctacgcga ccttcgatac cttccggcac cagtttgtcg gcagcgttat
     3661 cgctctggaa ataacggtca gaggaacctt tggacatcgc gcccagggaa cccataccac
     3721 ggtaagattt gtaagaacgg ccctggtaga gttcgatttc acccggagat tcttcagtac
     3781 ccgccagcat ggaacctacc atcaccgcgc ttgcgccagc tgcgatagct ttggcgatgt
     3841 cgccggagaa gcgaatacca ccatctgcga taaccggaat accggtgcct tccagggctt
     3901 caactgcgtc agcaacggca gtgatctgcg gaacacctac gccagtaacg atacgagtcg
     3961 tacagataga accagggccg ataccgactt taaccgcgct gcaaccagct tctgccaaag
     4021 cgcgtgcacc tgcagctgtt gccacgttgc cgccgatgat ttgcagatcc ggatatttag
     4081 cacgggtttc acggatacgc tgcaaaacgc cttctgagtg accgtgggag gagtcgatca
     4141 gcagaacgtc aacgccagcg gcaaccagcg cgtcaacacg ctcttcgtta cccgcacctg
     4201 cgccaactgc agcaccaaca cgcagacggc cttgctcgtc tttacaggcg ttcggtttac
     4261 gttccgcttt ctggaagtct ttcacggtga tcatgccgat caggtggaat tcgtcatcaa
     4321 ccaccagcgc tttttcaacg cgtttttcgt gcatttttgc cagcaccact tcacgggctt
     4381 caccttcacg cacggtgacc agacgctctt tcggcgtcat gtaaacgcta accggctggt
     4441 tcaggtcggt aacaaaacgc acgtcacgac cggtgataat acccaccagt tcgttttctt
     4501 cggtaacgac cggatagccc gcaaaaccgt tacgctcggt cagttctttc acttcgcgca
     4561 gcgtcgtggt tggcagaaca gtctgcggat cagtcaccac accagattcg tgttttttca
     4621 cacgacgaac ttcttctgcc tggcgttcaa tggacatgtt tttgtggata aagccgatac
     4681 cgccttcctg agccagagca atagccaggc gcgcttccgt tacggtatcc attgctgcgg
     4741 aaagcatagg gatattcaga cgaatagttt tcgtcagctg ggtgctgagg tcagcagtat
     4801 tcggcagaac ggtagaatga gcaggaacga ggagaacgtc gtcaaacgtc agagcttctt
     4861 tagcgatacg tagcatgggc aatatctcga ccagagtggt taataaatat tgccgcggca
     4921 ttatacagag cgtaaccgat tgcatctacc cctttttgca aaaaatgctt gctatccccg
     4981 aagggcgggt tactatcgac tgaataacct gctgatttag aatttgatct cgctcacatg
     5041 ttaccttctc aatcccctgc aatttttacc gttagtcgcc tgaatcaaac ggttcgtctg
     5101 ctgcttgagc atgagatggg acaggtttgg atcagcggcg aaatctctaa tttcacacaa
     5161 ccggcttccg gtcactggta ctttacactc aaagacgaca ccgcccaggt acgctgcgcg
     5221 atgttccgca acagcaaccg tcgggtgacc ttccgtccac agcatgggca acaagtttta
     5281 gttcgcgcca atattacgct ctacgagccg cgcggcgact accagataat cgttgagagt
     5341 atgcagccgg ccggtgaagg gctgctgcaa cagaagtacg aacagctcaa agcgaagttg
     5401 caggctgaag gattgttcga tctgcaatac aaaaaaccac ttccctcccc tgcgcattgc
     5461 gttggtgtga tcacctcaaa aaccggtgct gcgctacatg atattttgca tgtgttaaaa
     5521 cgtcgcgatc cttctctacc ggtgatcatc taccccaccg ccgttcaggg cgatgacgcg
     5581 ccggggcaaa ctgttcgcgc cattgaactg gcgaatcagc gcaatgagtg cgacgtgttg
     5641 atcgttgggc gcggcggcgg ttcgctggaa gatttatgga gttttaacga cgaacgcgta
     5701 gcgcgggcga tttttgccag ccgcattccg gtcgtcagcg ccgtcgggca tgagacggat
     5761 gtgaccattg ccgattttgt tgccgatctg cgtgcgccaa cgccgtctgc cgccgctgaa
     5821 gtagtgagcc gtaatcagca agagttactg cgccaggtgc aatcgacccg tcaacggctg
     5881 gagatggcga tggattatta tctcgccaac cgcacgcgtc gctttacgca gatccatcac
     5941 cgattacagc aacagcatcc gcagctccgg ctggcacgcc agcaaaccat gcttgagcgc
     6001 ctgcaaaagc ggatgagctt tgcgctggaa aatcaactta agcgtaccgg gcaacagcag
     6061 cagcggttaa cacagcggct gaatcagcaa aatccacagc cgaagattca tcgcgcgcaa
     6121 acgcgcattc agcaactgga atatcgttta gcagaaaccc tgcgcgtaca gcttagcgcc
     6181 acgcgtgaac gtttcggtaa tgcagtaacg cacctcgaag ccgtaagccc actgtcaacg
     6241 ctggcgcgtg gatacagcgt tactactgct actgacggca atgtactgaa aaaagtgaag
     6301 caagttaaag cgggtgaaat gctaaccaca cgtctggaag acggctggat agaaagtgaa
     6361 gtaaaaaaca tccagccggt aaaaaaatcg cgtaaaaagg tgcattaagc cagcacgaat
     6421 tcaacgcgtt ttttcgatat caagccatgc ccgtgctgac agaagtaatc tactgcaccg
     6481 caggctttta acacctgcaa cggttgatgg cagtctggac aaagagcttt catttcaata
     6541 atttccccgc aggatgggca acgcgcatgg tcgttatcct gatcaagcac atgctgacat
     6601 tgtggacaat gcagttccat atgaattcct ccggtagcca tccctctaca ttcatagagg
     6661 gaatggcaga taaaatactt acggataatt atttattttt cttgatgtgc ttcatcagac
     6721 gcttacgttt acgcatctgg gttggcgtca gggtgttacg cttgttcgca tacgggtttt
     6781 ccccttcttt gaactgaata cgaatcggcg atcccattac gtccagcgat ttgcggaagt
     6841 agttcatcaa gtagcgcttg taggaatcag gcaggtcttt cacctgatta ccgtgaatca
     6901 ccacaatcgg cgggttataa ccaccagcgt gggcatattt cagcttcaca cgacgaccgc
     6961 gtaccagcgg cggttggtga tcttcaacag ccatcgtcat gatgcgcgtc agcatagagg
     7021 tccccacacg acgggtggag ctgtcatacg cttcacgtac tgattcaaac aagttaccaa
     7081 caccactgcc gtgcaaggca gagataaagt gcacacgagc aaaatcgata aagcccagac
     7141 ggaagtccag cgtttctttc acctgctctt tcacttcctg gctcaggcca tcccacttat
     7201 tcaccacaat gacaagtgag cgcccactat tgagaataaa gcccagcagc gagagatcct
     7261 gatcggaaat accttcgcgc gcatcaatca ctaacatcac cacgttggca tcttcaatgg
     7321 cctgcaacgt tttgattacg gagaatttct ctacagcatc ggtgattttg ccgcgtttac
     7381 gtacgccagc ggtgtcaatg agcacatact cacgtccatc gcgttccatt ggaatgtaga
     7441 tactgtcacg cgtcgtgcca ggcatgtcat aaacaacaac gcgctcttca ccaagaatac
     7501 ggttagtgag tgtagactta cctacgttcg gacgacccac aatcgccagt ttgatcggca
     7561 gactttgcgg gtcgaagtcg tcttcctctt cttcttcgcc gttctcttcc gcttcaaatt
     7621 gcgcccagta ttcagcgtct tcgtcgactt cctcttgcgg tgcgagatct tccatccacg
     7681 gcagcagcac atgctccagc agacttaata cgccacgacc gtgagacgcg gcgatcgggt
     7741 agatttcacc taaaccaagc gagtagaaat caaccactgc ctgatcggga tccagaccgt
     7801 cagttttgtt tgccaccagg aaggtcggtt tttcacggga gcgcagatgt ttggcaatcg
     7861 cttcatctgc cggcatcagg cccgcgcgcg catccaccat aaacagtacg acgtccgctt
     7921 cttcaatcgc cagcagcgac tgttccgcca tgcgggtttc tacaccgtct tctgtgccat
     7981 caatcccgcc ggtatcaata cagataaact cacggccttc aatttccgca cgaccgtact
     8041 tacggtcacg agtcagaccc gggaaatccg caaccagcgc atctcgggtg cgagttagac
     8101 ggttaaataa cgtggatttt cctacgttag ggcgcccgac aagcgcgacc acaggtacca
     8161 tgtttaaagc ctcattttta taaatcatca gacaacgcac gctatattcg cgtcgttgtt
     8221 aaaaacagga aaacggcccc tgtccaggag ccgttttcaa agtgaacgac agagacgatt
     8281 aacgtgtaat agagtacacg gttccgtctt ttgcctggat cagcagtttg ccgtcagcgg
     8341 caaccggttc agtctggaaa ccggaactat caactttttg ctgggcaacg aaacgaccat
     8401 cttcgacgtt aatccagtgc agataacctt cactgtcacc taccaccagg ttgccattat
     8461 acagcaccgg agaagtcagc aggcgatgca acagatcgct ttgtgtccac agcgtaacgc
     8521 cgccatcaat ggtcaacgcc atcacccggt cattttgatc gaccagatag atgcgattgc
     8581 cgtcgacgat gaaatcattc accgaaccca gttcgcgttt ccacataatc tgaccactgc
     8641 gcagatcaag cgccgtcagg ttaccattat aggccagcgc gaaaacaacg ccgttaacaa
     8701 cgacgggagt cgtgtcaaca tcgctcagac ggtcaatttc ggtagaaccg gtcgcctggg
     8761 aaatacgctg ctgccaaatc atctggccct gttccatcag cactgcgctg acgcgaccat
     8821 tatcgccacc cacgacggcc gcaccaaaag ccgttgccgg cgcagactcg ccacgcaaag
     8881 agagcgaagg catatcgagg ttaactgtcc atttgacagc gccgtcagcc tcgttcagcg
     8941 cttgtaactg accgttactg gtgtggatta acaccagacc gtcgctgacc accgggcgcg
     9001 aaagtgcttc acccgcgact ttagtttgcc atgccacagt accatcgctg gtattcagcg
     9061 cgtaaacctg cgccttttcg ctgccaatgt agacatgccc accagacacg gtcagaccgc
     9121 cagaaagtaa tgcaggctct ttagagaacc agccatcttt ctcggccagg ctgacagacc
     9181 agatttcttt gccatcatcc gcattcagcg cttttaccaa accagcgcgg tccgctgcat
     9241 agacaacgtt gtccgccagt gccggatgaa gattggaata gaagttgcca atgccgctac
     9301 caacggaagt gctccacgcc gtggtcggcg taaactggtt ttcaacggtt ggcaatgggg
     9361 acatctttac cacatcttct tcgctgttaa acagcgaaca gccgcttaaa agggtaacgg
     9421 aaagcagtcc tggcagcagt aatttacgca attgcatcgg gtccctctca gatggacaaa
     9481 ttattaattt tcatctgcat catttcgctc agtgccggag taacatcgct tttcacgcct
     9541 gcttcccatg cactacgcgc accttgctta tcacctttgc tcagcaatgc ttcaccacgc
     9601 aggtcggcaa caatggcagc ccacccttca cctttgatgg tatcaagggt tttcagcgcg
     9661 gcatcagcct gcttgagctg tacctgaacg cgagcaagac gcagatttat cacggctttg
     9721 agattttcat cgctcgtgtc tgccagcccc tgttgtaact gggcggcagc tttctctagt
     9781 tcatttttgt caacaaattg ctgcgcaagt tccaaagaag ccagcgcacc ataagtattt
     9841 ttattttcag cagcaaattt ttccgccgcc gggatgctat ccggtttgcc ttcgctcact
     9901 gcggtaaccg cattttgata ggcaagagaa gcggagcgtg cagaatcaac ctgatggctg
     9961 ttccagtagc gccagccaat cagtgcgcca acgcccaaaa tcaccccaac agccagtgct
    10021 ttgccatttt cagcaaaaaa gcgtttaacc gcttctacct ggtcgttttc gttctcgtaa
    10081 atttccacgc tgtccttctc cttccttaac ccagtaacgt gcgcaaatgc gcggctacgc
    10141 tatcctgcgc aactgccgtt tgctcaccag agcgcaaatc cttcactact gctgtgccgt
    10201 tagccacttc agactcaccc agcaccacag caacgcgggc accccattta tcagcacggg
    10261 caaactgttt cttaaagttg ccgccgccgt ggttggtcat caatttcacg cccggtaatt
    10321 catcacgcag acgctcagct aatgccatag ccgcagattg tgtatcagca cctgaagcca
    10381 ccaggtatat atcgacaaca ggatcggctt taaattccgg attaacggcc tgtactaaca
    10441 atacaagacg ttcgaggccc atcgcaaaac cgacagccgg tgttgcacga ccgcccagtt
    10501 gttccacaag accgtcataa cgaccgcctg cacacacggt gccctgggag ccgagactgt
    10561 tagtcaccca ctcgaaaacg gtacggttat agtaatccag accacgcacc agacgctggt
    10621 ttacggtgta agcgatcccc gcgctctcaa gcagtttgca cagaccggca aaatgctcac
    10681 gagattcctc gtccagataa tcacctaatg ccggagcgtc gttgagaagc gcctgcactt
    10741 ccggattttt tgaatccagc acgcgcagcg ggttagtgta catgcggcgt ttgcagtctt
    10801 cgtccagctt ttctttatgc tgctcaagga atgccaccag cgcatcgcgg taattggcgc
    10861 gtgcttccag cgaaccgata gaattcagct caagagttac gtgttcggaa atacccagcg
    10921 cgcgccacca gcgggcggtg agcataatca gttcagcgtc gatatccgga ccttgcagac
    10981 cgaaaacttc gcagcccaac tgatggaact gacgataacg ccctttctgc ggacgctcgt
    11041 gacggaacat cggcccgata taccacagac gctgttcctg attgtacaga agaccatgct
    11101 cgatgccggc gcgtacacag cccgccgtcc cttcagggcg cagagtcagg ctgtcgccat
    11161 tgcgatcctc aaaggtgtac atctcttttt caaccacgtc ggtgacttca ccaatcgcac
    11221 gtttgaatag cggggtctgc tctacaatcg gcaagcggat ttcactgtaa ccgtagctgc
    11281 cgagcacgtt tttcagtgtg ccttcaatgc gctgccagat ggccgtttcg ccaggcaggt
    11341 aatcgttcat gccgcgaatg gcttgaatgt tttttgccac gtttattctc tttctgaata
    11401 taaaaatgaa ccctcaacgc ttccctcaat gtttcgggag ctatgcgggt tcaatcatac
    11461 acgggaagcg aggcgcttcc catcacgtta ttatttttca acctgctgaa cgtcaattcg
    11521 acgcgcttcg tccagctgac tggctttcgc acgaatgcgt gcttccagct ggtcgatcat
    11581 atcgttgttg tccagacggt ctttgcgcac gccatcttca tagaggccgc ttttcttgtt
    11641 gccgccggtg acgccgagtg tagaaaccag cgcctcacct gggccattca ccacgcagcc
    11701 gataatcgaa acgtccatcg gagtgatgat atcttccagg cgttgctcca gcgcgttaac
    11761 cgtaccgata acatcaaatt cctgacgcga acaggtcggg caggcgatga agttgatccc
    11821 gcgcgaacgg atacgcagcg atttcaaaat atcgaaaccg actttgatct cttcgaccgg
    11881 atcggccgcc agtgatacgc gcagcgtgtc gccgatgcct tcagacagca gcagacctaa
    11941 accaatggcg gattttactg ccccgctgcg tgcaccaccg gcttcggtga tccccagatg
    12001 cagcggctga tcgatctgtt ttgccagcaa acgataagac tcaacagcga ggaagacgtc
    12061 agacgctttc acgctgactt tgaactgatc gaagttcagg cgatcgagat gatcaacatg
    12121 gcgcatggcg gattccagca acgcctgcgg cgtcggttcg ccatactttt cttgcagatc
    12181 tttttccagc gatccggcgt taacgccaat acggatcgga atgtttttat cgcgcgcaca
    12241 gtcaaccacc atgcgaatac gctcttcatt accgatattg ccagggttaa tacgcagaca
    12301 atcgacgccg tattccgcta ctttcagtgc aatgcgatag tcgaagtgga tgtcagccac
    12361 cagcggcacg ttaacctgct gtttgatgag tttgaacgct tctgccgcgt ccatcgtcgg
    12421 tacggagaca cggacgatat cagcgccaac gcgttccagc gccttgattt gattgaccgt
    12481 tgcttcgacg tctgtcgtac gcgtattggt catggactgt acggcgatgg gagcaccatc
    12541 gccaatcggc acattcccaa cgtaaatacg tgttgatttt ctacgttgaa ttggagcctg
    12601 gttatgcatg aaaaatctcc cgcgttaccc gtctgttact gcgccggtga ttgttcggca
    12661 ttgagggtca gacgcgcaac ctggttagtt ctgataaaac gactcagatc gacaggtttc
    12721 ccttgatact ggatctgtac tgcggctggc gcaccaattt tcagtttgta tggtgcctgg
    12781 ccggttaagt tcaaattacc gtctttacgc tgcataccgc taaacaattt tttaccggta
    12841 gcatcagtaa cctccagcca gcaatcggca gtaaagttca tcaccagcgc attcggatca
    12901 gccgccggcg tagtcacgcc tgcctgatcg gttggcaacg gcgcagcgcc atctggcgtt
    12961 gttgttgccg ttggtgccgg ggtcgcggcg gtatcaacat ttgcctgcga aggcgaaaca
    13021 accgcattct gttgcggatc aacagctggt gctggcgcag ttacggcagg tgtttgcgtg
    13081 ttggttgcgg tagtatccac agacgctggc ggcgtggacg ttgtagccgg gtctgtagtt
    13141 gtcgacgtat ttaacggaac actctgcccc tgctcgctat tactgctcag ttccgccgaa
    13201 gattgatcgg ccatagtggt gatctcttcc tgctgagctt tgtggtcttg ccaccaccag
    13261 gcaccgctca ggccgataac cacaaacaac accagccagg tgaaggtcat cagccagccg
    13321 tcacgttttt tgcggcgttt accgagggaa aaactctgca taggcgcaac ttttgcagcc
    13381 cgtagtggag cctgcttttc cagccctggc agcagttctt cttctggaat atgtaccaga
    13441 cgcgcataag agcggatata tccgcgcagg aatgttgaag caagatcggc gggtgcctta
    13501 tcttcttcaa tgtcgcgtac cgtggaaacc ttcaggcaaa gtcgctcggc aacggcctgc
    13561 tgactaagtc ctagttgttc gcgagcatta cgcaggcgag cgccggtagt aagtgcttca
    13621 ttttggtcgt gcgtggcttc agtattcatt cgctgcaggt acgtttaaat gagaattgag
    13681 atgccggtga accatcatgc tcacccacac cgcgaaacat ccgttaagtt aaccgttatc
    13741 atacagtata agaccgtctg tgtgctcgtg acaaagccca cattcactaa ggctaactta
    13801 ctgttgcatc gttacatact gccttaaagt cagcaaaaac gcaccgttaa tattgaccag
    13861 acaattgcaa cttaatactt cattcataaa agattacgcc acggtacata aagtaaccgt
    13921 ggcgtaatgg ctatcagacc gctttaatgt cgatggcttc accctgcatc cgtttacgca
    13981 gggtacgttt cgtacggtcg ataacatcgc ccgccaactg accacaggca gcatcgatat
    14041 catcaccacg ggttttacgc acaatagtgg tgaaaccgta gctcatcagc acttttgaga
    14101 aacggtcgat acggctgttc gagctgcgtc catacggcgc gcccgggaag gggttccacg
    14161 ggatcaggtt gatcttacac ggcgtatctt tcagcagttc cgccagttgg tgcgcgtgtt
    14221 cagtgccgtc gttaacgtgg tcaagcatca catactcaat agtgacacgg ccctgattgg
    14281 cgttggattt ctccagataa cggcgcaccg cagcaaggaa cgtttcgata ttgtactttt
    14341 tgttgatcgg cacaatttcg tcacgaattt cgtcgttcgg cgcgtgcagg gaaattgcca
    14401 gtgcaacgtc gatcatatcg cccagtttat ccagcgccgg aactacaccg gaagtagaaa
    14461 gcgtgacgcg acgtttagac aggccaaaac cgaaatcatc aagcatgatt tccatcgccg
    14521 gaacgacgtt gttcaggttg agcagcggct cgcccatgcc catcatcact acgttagtga
    14581 ttgggcgctg accggtgact tttgctgcgc cgacgatttt cgccgcacgc cacacctggc
    14641 caataatttc cgatacccgc aggttgcggt taaagccctg ctgggcggtg gaacagaatt
    14701 tacactccag cgcacacccc acctgcgaag agacgcagag cgtggcacgg tcgtcttccg
    14761 ggatatacac cgtttcgacg cgctgatcgc caacggcgat cgcccattta atggtgccgt
    14821 cggatgaacg ctgttcttca accacttccg gtgcgcggat ttccgccacc tctttcagtt
    14881 tgccgcgcaa cactttgttg atgtcggtca tctcatcaaa gttgtcgcag caatagtgat
    14941 acatccactt catcacctga tcggcgcgga agggtttttc acctaaatct ttaaaaaact
    15001 cccgcatctg ctgacggttg agatccagca ggttgatttt tccatctttc gtggtgacgt
    15061 tttcaggtgt gactaattgt tcagacatat gctattccgg cctcgttatt acacgttatg
    15121 gcccctggag gattgaaaaa agaaacgccc cggtgagcgg ctgctcgtcc gggggcgctg
    15181 cattgtacaa attctggcgc acggatgcca cgtttgcacg cggcatttac gaaattatta
    15241 acgggtgcgc gggcacactt cgccttcgcc aaagaaataa gcgatttcgc gagcggcaga
    15301 ttcgacggaa tcagaaccgt gggtaccgtt ttcggtcagg ctgtcagcgt aatcagcgcg
    15361 cagagtacca gccagtgcgt ttgccggatt ggtcgcgccc agcagatcgc ggtgacgctg
    15421 aacggcgttt tcaccttcca gcacggaaac cacgatcggg ccagaggtca tgaattcaac
    15481 cagaccatca aagaacggtt ttccatcgtg ttcagcataa aagccacgtg cctgttcaac
    15541 ggtcaggtgc agcattttgg tgccgacaat tttgaaccct gcagcttcaa agcgcgcaaa
    15601 gatattacca atgacgtttt ttgctaccgc gttcggtttg atgatggaaa aagtacgttc
    15661 aatagccatt tttacctctg taaattgttc tgttgttgtc tgtaccagcg tacgagatgg
    15721 cgcggattat aatgagcaac agggccgttg actattgatg aaggtaacat ttttttaaaa
    15781 taaaatgagt tttagcaaca atcagggaca aaacagacta ttgcatgaca aatttcactg
    15841 tcgcgatttg ccccacatca tccatcacca gcaattgata atcaccttta tccgtcaaat
    15901 gcagggtcac gttgcgcccg cgttcagtta acggttcgcc attcagaaac caccagcgtt
    15961 cacctgcccc tccacttgat tgcaacggca aagtcgcttc cgctgcgccc ggtaaacgtt
    16021 taataatcgc gccatcgcga acgcctgtca gctgcagagg gagctgggca tcgtgaccgt
    16081 atgacgggca aattgtcgag gccggtggta agcgcacagc gcgacgttct gatgcgggca
    16141 gccagggttc cagcggtagc ggccagacat ttatcatttc ttgtcgcgct tgcgggcaat
    16201 cagcggcaac acgtttgcca ttttcatcca gccagatggg gaaacgaatg ccattaatgc
    16261 cttcctgctc cggcagtaat agagttggcg gctgactccc gtccagcagc caggttgcca
    16321 ggcggcggcg acagttaccg tcaccttccg gcaaagactg tccgcccggc cagcagataa
    16381 cgccacgagt gactgagttc gggcgcgggt cttccggcag attcgcactg cgcgacagta
    16441 agatattatt gacctgattc aacaatggta cggcactggc aaagccaaac tgaccaacaa
    16501 cgggcgtgcc gtccggtctg ccagtccaga tcccaatgac atagcgagcg ttaaccccaa
    16561 tcgcccaggc gtcacgatag ccatagctgg tgcccgtttt ccatgccagt ggggcgacgc
    16621 gcggcaaggc actatccggc aagggtttcg cttcatcagc cataatccgg cgaatgatcc
    16681 acgccgcccc cgacgacatt aaaggccgtt caagcagcgg atcgtcaggc tgtaagcgca
    16741 atttgcctgc cttgccgtgg cgagcaaacg cggtatacgc tgccgccata tcttccagtt
    16801 ttgcaccagc gccgccgaga atgagtgaaa gattcggcgc agcaccgttg ggcaaatata
    16861 acggcaatcc aacattgcgt aactttgccg caaaccgttt cggtccatag gcttccagca
    16921 cctgcacagc aggtaagttc agcgagcgca ccagcgcctc gctcatgctg atcgggccat
    16981 gaaaaccgct atcaaagtta cctggtcgat aatcaccggt gcgccggggg acgtcttgca
    17041 gcagtgatgc cgggtggatc aagccttcat ccagcgccag accataaaca aacggtttga
    17101 gcaccgatcc tggcgatcgg ttcgcattga ccatatcaac atgaccaaag cgtgaatcat
    17161 cgttgagatc aaccgatccc acccagccgc gaacacgcat atcggtatga tcaaccacga
    17221 tcatcgccag tgagctgcgc ggtggcaacc gccctttcca gttttgcgcc agttcttcca
    17281 gacgtcgttg aagaccggca tccagcgtag taacgatttt gtcgcttttg cttttaccga
    17341 gcatcatgcg cgaaaacagc ggtgccagtt gcggcatttg tcggggtgcc agccagatgg
    17401 gttcttccct tgactctttt acctgctcac gggaccacgc accttgcacg gccatccgtt
    17461 cgagcacttt attacgcgcg gcttcggcac gctccggcca acgatccggg cgaagacggc
    17521 tgggcgcttg cggcaaaacc gccagcattg ccgcctcgga atagcttaaa ttcgcaggcg
    17581 actttccgag ataagcccaa cttgccgcac cgatcccctg caacgtaccg ccaaacggag
    17641 cgcggttaag atacaaggtc agaatttcac gcttagacag atgccattcc agttgcaacg
    17701 cgcgccagag ctggcgaatt ttgccgccaa atgttttggg gtgaggatca agcagacgag
    17761 caacctgcat agtgagcgtg ctgccaccgg aaataacccg tcccgaagtg agatcttgcc
    17821 atgctgcgcg cgccaccgag aatggattca cccccggatg cttccagaac cagcgatctt
    17881 cataattgat cagcgcttca aggtaacgtg gagaaacatc ttcgattgtt accggataac
    17941 gccagatgcc gtcagcatcg gcgaagcgcc atagcggcgt accatcctgc gccacgacca
    18001 ctcgtgcggg attgacttca tgcagcggta gaggccagag tttatctgct ccccacgctg
    18061 caagaaaaag gagaaatggc gcagctgcca gcgttatcca gcagccgcgt ttggttaaca
    18121 agcgaggcat ttacggtcgg acaatcagca gatcttcagc cgcgccggtc gcccgccatt
    18181 ggggaacata cattgattcc accatcggtt gcggtacctg atacgttccc ggcgtcaccg
    18241 cccgcgccag atacaccagc gttaccggtt ggtattcatc aacggcaacc gccgccacaa
    18301 agcgatcgtc acggaactca atgtgcttaa tactcgcctg ctgcatctgg ttcagtaagt
    18361 tctgcacttc gccaccactt tgctccaggc tggcgctacc gttcgccaga ttctggtttt
    18421 ccagttccag acccgcaggc agcagatcca cgaccaacgc atccggcacg ctgttactgg
    18481 cttttacctg caaccacacc agcaccagat cgccgctacg taacgagtcc agcgatttgc
    18541 tcttaccatc agtaccaaga atatgacgct cgatttgcag cacatcgttc gccggtaaag
    18601 gtgcggattg cggataaccg ctggcatcca cacgcagcca taacggctga tcgccactgt
    18661 ttctcacctg caaggtggca agttgatcgc tattcagatt gctgtttagc gttttctcgc
    18721 ctgtcagcgg ctcagctgag aaagaggttt gcgcctgcca tttacccggt aaatcctgaa
    18781 tcgtacgggc agccaggaac aacgcgttac tttcctgcgt cgatagccag cgttcaccaa
    18841 atgcctgctg cgaaagcgtg ttcagcaaag tgttctgctc atcgggtagc agtttatttt
    18901 cttccagcaa ggagagcatt aacgcgttgt cgcgcagtgg actaccgtaa tcacccagcc
    18961 atatccgctc atcactattt ctcggcgttt tcagcgccag cacaatcgct tcttcaccac
    19021 gcgttgcatc acccatggtt ttcagcgcaa cgccaagttg cagcagcggt aaaccagaag
    19081 cggcatctgc acgatgctcc cagatttcac gcagcgcacc cagtggtgct ttttgctgac
    19141 gggccagcac cagcgcagcg taagactgaa cggcgaattt actggctttg agattatccg
    19201 cgtacgggat cgacatcatg cccggatctt gtaaatagcg cagcagacgc tcattacccc
    19261 ggttaatggc gtctgtcggc acgctgtaac cctgttcgcc tgcgcggacc aggaaatcca
    19321 tcacgtaagc cgtcagccag tactcttcgt caccgttttt atcccacagc gcaaagccgc
    19381 cgttatcacg ttgcatttgc agcaaacggg aaatgccgat atcgaccgat gcacggcgtt
    19441 tctcatcact gtcgcctttg atgcccaacg cctgcagttg ggctgcgttg gtataaagtg
    19501 acgggaacag gccgctggcg gtttgctcaa gacagccgta cggatacgct tttaactctt
    19561 tgatataacg tgcaatgttc agcggtggtt tgccgctcaa caacaattgc ccttccagcg
    19621 taacaggcga gaagttttgc aatccatccg ccggaatcgc ccatgtctca ccaggctgta
    19681 acgccgtacc gtaattaacc gtttgtgccg ggaacgccgg acggacgccg attttccact
    19741 gcttatgctg atcggcaacg gtttcacccg gtaacgctaa cccgctaatg gtggcctgaa
    19801 tttctccatc gccataaccc ggcaatgctc gcaccgggat aaacagcgta gtacgcacac
    19861 ctggcgctaa ttcaacggct gcgggtgaat cgctgaccag ttcaagcaaa ccactggcgg
    19921 tcagggcaac gttcagtttt tgcggtttat cggtaagatt agtgatatcc agcgtcagac
    19981 gcgaggtatc gccactcgcc ataaagcgcg gcatgttcag ttcagcaatc accggtgcgg
    20041 caactatcac tttgctttcg ttgctaccga aatcatctgc cgtccaggct tgcgccatga
    20101 cgcgcaattc accgttaaaa tcgccaatcg gcagtgtaac cgagccttcg ccctgttcgt
    20161 tgagcgttac cggcagcgcc tgctgcgcga caatattgac gtgattgacc ggcggtttac
    20221 caccacgttt cagctcatca ccatcgccac cgaaacgcag agctgccaga cgcccctgac
    20281 cttcaataac ctgaccgtaa atatcgtaaa tgtctgcgcc atagcgtttc tgaccaaaga
    20341 acgcctgcca cggatctggc gtgacgtagt cagtaatatt cagcacacca ctatcaacgg
    20401 cagacaccag cacattcacc tgtttaggct tctcgccatt tttagtgctg gctttaattt
    20461 tcacggttaa tggttgattg ggacgcattt ttgctggtgt ttccagcgcc agatcgaggc
    20521 gacggttttc atcgccaagc ggcagatgca acacaccaac cgcgcgtttt ggcgtcgcgg
    20581 agcgagattt atcgccagga cgtaccacca gcgtacttaa atacagatca tgatgattcc
    20641 aggttttatc gaccggaatc gtcagatcca gcccttgagc ccgaacatca atctcttgcc
    20701 accacagcgg cccttcactg gactcgacca tcgcataacc tttacccgcc gttggcgcgg
    20761 cgatatgcaa cttaatggtg tcgccagggc gataactggc tttatccagt ttcagcgtga
    20821 cacggtcggg tcgcactgcg ccgctaccgt cgctgttgtc ctgccagcta tagccagccc
    20881 agaaacgaac actactgacc gcttcattcg gcgctttgac ttccagacga taagcgcccc
    20941 actctaccgg aaaactgacc ttgccggttt cgtccgcttt cagatccaga gtttgttcat
    21001 tttcgatcag atctttttga tcaaactgtg actgccagcc ttcatcttct gaccagttcc
    21061 agtagtaatc gcggcgttcg cgaatcaggc gcacctgcaa gcccgacacg gcttttttca
    21121 cgccttgcgc atcgctataa acgatgtcaa aagcggcgtt actgccttca tcaacaatgg
    21181 gctgtttcac cgtgctgtca gtacgataat cgtaaaccga tttcgaggcg aactgcggac
    21241 ggatccccgg caatgcatcg gcaggccaga tagcctgctc agcgcggcgc gtcaccgggc
    21301 gaccgcccga ttccagcaaa ctaccctgga aaataacctg taatggggaa tgcgtttcct
    21361 tccactggct ttctgtagaa acttcgccgc gccctttatc atccagcgtc aactgaactt
    21421 catccagcgt gcgggaaaga ttttcggcag ctatatcgcc gaattcaaaa ccaggtaagg
    21481 ctgacacagc ttcacgcagt gggcgcagga aaagttgccc ttgcaaagta ttaccattag
    21541 caggtgcacc atacaggtag taccccacca cggagaattt cacttcatct ttcggcgtta
    21601 gcggggtttt ctcaccggtc agattcagcg ccatgcgctc tggcataaaa tcttcgacgt
    21661 ggaaatccca catccgatac tggttatcgc ccgtgttagc gcgaatatgc cacataccgg
    21721 ttgccgcatt gctatcgagt ggccaggtaa agtggtagag gccattctcc ggctgactaa
    21781 cgacgctcct gagtacctgc ccatcgggtt taatcacgtc taacttgatg ggttgattgg
    21841 gcaacgcttt accgtctgca tcacgcagca aaccattgag gattaccgtt tcacttgggc
    21901 gataaagatc gcgtgggcca aacatgaaaa actgtttgct atagcctggc gcgccagcaa
    21961 tgttaaattc tgctaagtcc agcgccggaa gttttaaatc gagtagcgtt gtctgaccgt
    22021 ctttacgcgc caacagtaat gccgcgtttt tatcattttc cagctgcaca tgcccctgag
    22081 cgtcactggt tgcctgagtc agagtctgcc ctttctcatt taataaagag acttcaattc
    22141 cttgctgggc cgcgccgttt tccagacttt gggtaaagat atccagacga ttgtgataac
    22201 ggtgagctga aacgccgata tcacttaacg taaacagcgt cgcgggatta ctgtaatcgt
    22261 aacgtccagc ctgattcatc acagccagat acacgcccgc ctgctgaagc ggtttgatat
    22321 cgcccagcgg cagcaataat ttttcacggg tgttacgcgc aggattgaga tcaaaccgtc
    22381 cggtgtagac cagatccgcc atctgcagca gtttgtctga ctgccagttc gccagcgaat
    22441 tgcggtattc ccattggcta atgaatgctg gcagagattc tggcttaacg cggaagaagt
    22501 taacatcgac attattaacg ttgagcgcca ttaccggcag cccttcaacg actttgccag
    22561 gcagcagcga accacggctg gcaaaaccga cgctgggttg gatgtcgcgg gtagttatag
    22621 ttttttcgta atctttactg aaggttgcgt tgttgagtgc tttgacctcc ttgccaatag
    22681 taacgatcaa atcacgtttc ggttcgaggt ggcgtaaacg taactcttta agattatctg
    22741 acagctccca ggcaccatcc actttgccgc tttttttatc gacgacatga ataacgcgtg
    22801 agaaatcctg atccgggtcg agagggatgg agaacgtcag caccagcgtg gctgcaccat
    22861 caagttggac ttcagagaga tccagcaatg tcagcacctt accggcactc tgttgggcca
    22921 gtttttgtct ctccggcacg gagagttttg ctgaactcgc gttttctgaa gaggccgctt
    22981 tagtaacttc agaaggcgca tcttttttca ccgctgttgg cgcgttatcg ttgttgtcgc
    23041 accctgccag cgccagcatt agcatgcagg cggctacgcg taactttttc atttttcatc
    23101 cctgccacaa tggcccgtta gcaacgtcga ataattatta tgcgtgagaa tttacgttat
    23161 gtaattcagt atcacgccca tacaatccga attttagtga gaattgtggc tcagacagcc
    23221 aaatcagcct ctttcgcttg tcgcttttca ctaaacatcc gacaatttag cctaaccccg
    23281 gcaaaaatgg agatgcctat gtccacgaca tggtttgtag gagccgactg gctcgccgaa
    23341 catattgatg acccggaaat tcagattatt gatgcccgca tggcgtcgcc tggacaggaa
    23401 gatcgtaacg ttgctcagga gtatctgaat ggacatattc ccggcgcagt gttttttgat
    23461 atcgaagcgc tttctgatca cacttccccg cttccgcaca tgctgccgcg cccggaaacg
    23521 ttcgccgtgg cgatgcgtga attaggcgtt aaccaggata agcacctgat tgtctatgac
    23581 gaaggtaatc ttttctcagc cccacgagca tggtggatgc tgcgcacctt tggtgtagag
    23641 aaagtgtcga ttctgggggg tggacttgca ggctggcagc gcgatgatct gctgttagaa
    23701 gaaggtgcag tagagctgcc ggaaggagag tttaacgccg cgtttaatcc tgaagccgtg
    23761 gtgaaagtaa ccgatgtatt attggcaagc catgaaaata cggcgcaaat tattgatgcc
    23821 cgcccggctg cacgttttaa cgcagaagtt gatgaacctc gcccaggttt acgtcgcgga
    23881 catattcccg gagcactgaa tgttccgtgg acggaacagg tgcgcgaagg cgaactaaaa
    23941 acgaccgatg aactggatgc gatatttttt ggtcgcggcg tcagctacga caaaccaatt
    24001 atcgtcagct gcggctctgg tgtaacggca gccgtggttt tgttagcact cgcgacgctg
    24061 gatgtgccaa acgtgaaact gtacgacggc gcatggagtg aatggggcgc gcgggcagat
    24121 ttaccggttg agccagtgaa ataagtattt tacaggcaat aaaaaaccgc cgaatttggc
    24181 ggttttttat tgctggtctg gttcgcggcc tttccagcag gttgtattac cgtagtaatg
    24241 caagcgcgtc tcagcggaga caatactcgc cagtaactct ctttttgtca agcaaaagag
    24301 agttattatt gttctgttag tgtattatcc actgcggccc tttccgccgt ctcgcaagcg
    24361 ggcgctggct taaggaaagg atgttccgcg accgttagag caagcgcgtc tcagcgcttg
    24421 ctatcgcggc aatatcgcca gtggtgctgt cgtgatgcgg tcttcgcatg gaccgcacaa
    24481 tgaagatacg gtgcttctgt atcgtactta ttgtttctgg tgcgctgttt tcccaagtaa
    24541 ataataaccg gagtctctcc ggcgacaatt tactggtggt taacaacctt cagagcagca
    24601 agtaagcccg aatgccgccc tttagggcgg catattttag attatccggt tctgtttaaa
    24661 gtcacgcaaa aaaccacccc agcgacgttc atagaatggc gcaatatgtt cggtaataaa
    24721 gtggctaatt cctttttccc cttttttcac ctgacaaata tcgattggtt catcgccagg
    24781 taatgtatcg gtcgctacac ttcccgtcgc ctgaataatt tcttcgatat caccatcggc
    24841 ttcaatgcca ataagtaaat taggctgtgc ctcttcgttc tctttaattg aacaaataaa
    24901 agcacgcttc accggcttaa tggttttaaa taaggtggtg agtgaatcaa tcatttgtgc
    24961 tggcggctct gcgacttccg ataatatcag cgattcaccg ccttccagga tttcctggct
    25021 gctcagcgga tttccctctc caccaatcaa caaactgatt tcacgcggca taaattcttt
    25081 accggtcggc agtttggcat taaggaagag cgtttcgcca agggtcatct caaacaatgt
    25141 gcgaacgggc attacgacaa atgcctgttc gtcttcaacc gcctgttgaa gcgcttctaa
    25201 cgaggtgaaa aaaggaatga cgctggtgcc gtcttctttt tcccagtgct gtaaatcaag
    25261 cgcactatct tcaaccacag cctcgccctg cgccgccgta ccaggcaccc agacggtgga
    25321 ttccagtaga gtacggaaaa agcccgggcg gtgcgccggt tcggttgctg ctttttccag
    25381 caggtcttca agttcgtttt ttgtttcgga catatgaacc acaattcatt caacgttcgg
    25441 ggcgcaaatg cctgatgcgc tacgcttatc aggcctacaa gggattcgca atttgttgaa
    25501 tttgcagaat ttgtaggccg gataaggcgt ttacaccgca tccggcatat tagtttacgc
    25561 cgttaacaga ttagctatcg tgcgcacacc aagtcccgta gcgcccgcag accactgttc
    25621 aaccggcgct ttacggtaag tcgccgagca gtcgatatgc agccagcctt gctgatagtt
    25681 ctcaacaaag tgcgacagga agcccgccgc cgtgctcgcg cctgccggat acgccgcgct
    25741 tccggtattg ttcagttcgg caaagttaga cggcagctgg ctgcggtgga attccgccag
    25801 cggcagacgc cagaatggtt cgttctcttg tgaggcactc gccagcaaac gaccggcaag
    25861 cgcatcgtca aaactgaaca gcgcgtgata atcattaccc agcgcagttt tcgccgcccc
    25921 ggtgagggtc gccgcatcaa tgatcatttc cggtttctgc gcactggcat caatcagacc
    25981 atcggcaagc accagacgcc cttcggcatc agtgttcatc acttcaactt ttttaccgtt
    26041 gcgataggtg atgatatcgc ccagcttgaa cgcattgccg ctaatcaggt tatccgcaca
    26101 gcagaggaac agcttcacgc gcttgttcag tccgcgcgta atggcaaatg ccagcgcccc
    26161 ggtaaccgtt gccgcgccgc ccatgtccga cttcatcgag tccataaacg cagtctgttt
    26221 gatgctgtag ccgccggagt caaaagtgat acctttacct accaggcacg cgtacaccgg
    26281 tgcttcttta tcgccagttg ggttgtaatc cagcgccagc aataccggag aacgttctga
    26341 accgcgcccg acggtgtgca gccccatata accttgctca cgcagatctt cgcctttggt
    26401 gatccgataa gtcacacgat cgcccgcgac gttgctgatc agatcaacag cacgctgtgc
    26461 cagttgcgat ggtcccaatt cttctgccgg tgcgttgatg gtgtcacgca cccagtcgat
    26521 gatcatcagg cggttatcca gttcctggcg ctgggcatcg tccagatccg gccactccac
    26581 tttacgcgtg cctttcgggg ctttgtaacc ttgccagaat gcccagcagc gatccgcatc
    26641 ccagccttca ccgcttaact gaacatgctt gatgcccaga ccgtcaatct tgcgcgccgc
    26701 acgctggatc agccccagat cgtctgcccc gttcaaatgc agggtaatgc cgtcattatt
    26761 aatgctgtaa gttgcttttt ctccccagcg cgcgtcggca ggttgggtag agagggtaat
    26821 cttcatcgct tctgtcattt tagttatcct tctttgtaaa agggccgcca gttggcagcc
    26881 ccaggatttt tccgctaaat gattcgcgtt gcaggaaggc ggcaagtgag tgaagcccca
    26941 ggagcataga taactatgtg actggggtga acgagcgcag ccaacgcatc tgcggcgtga
    27001 agcatgacgc ggaaattatt cggcctcgtc cagccagact aacaaaatcg cttcgaggat
    27061 tttctcgttg gatgcctgcg ggtcgtcgtc gaaatcttcc agatcgcaaa tccactgatg
    27121 catatcggtg aatcgaaccg ttttcggatc aagatcggga tacgcatcgt acagtgcttc
    27181 gccaatttcg cggctatcgg tccatttaag tcccatacta acctctgtta atgctcacgc
    27241 gcatggttga tagtgtaacg cgggatttcg accactaaat cttcgtcggt gacgcgcgcc
    27301 tggcagctta aacggctttc cggctccagt ccccaggctt tgtccagcat gtcgtcttcc
    27361 tgctctgagc tttccggcag cgagtcaaaa ccttcacgaa cgatgcagtg gcaggtggtg
    27421 caagcacagg atttttcaca ggcgtgttca atctcgatac cgttacgcag agctgcgtcg
    27481 agaatggttt caccgctatt agcttccaga acagcgccat cagggcagag atcctgatga
    27541 ggcaaaataa caatctttgg catattaaac ctcgtccacg gaatggcctt tcagcgcacg
    27601 acgaaccgac tggtccatgc ggcgagcggc gaaatcctgg gtttgtttgt ctacgttttt
    27661 aatcgcttgt tcgatggcgt caacgtcatc gccctgcgcc acttcactca ggtgagcggc
    27721 agcatcgtca atgacctgac gttctgcggc gcttaacagc gcggcatcag cagccagcgc
    27781 gccgtgcaga ctttccagca cacgcgccgc ttctactttt tgttctgcca gcattcgggc
    27841 ttttacgtcc tgctcggcat agctcattga gtctttgatc atcgaagcga tttcgctatc
    27901 ggtcagaccg taagacggtt tgacctgaat agacgcctca acgccggtgg atttctccat
    27961 cgccgtcacg ctcaaaagac cgtcggcatc gacctggaac gtcacgcgaa tatgcgcacc
    28021 gccagccggt agcgccggaa taccacgcag cgcaaaacgc gccagtgagc ggcagtcctg
    28081 caccagttcg cgctcaccct gcattacatg gatagacatc gccgtctgac catctttaaa
    28141 ggtggtgaaa tcctgagcgc gggccaccgg aatagtggta ttacgcggaa tcactttctc
    28201 caccaggccg cccatcgttt cgaggcccag cgacagtggg atcacatcaa gcaacagcat
    28261 ttcgctgtct ggcttgttac ccaccagaat atccgcctga atcgccgcgc caatagcgac
    28321 gactttatcc gggtcgatgg aagtcagcgg tggacgaccg aaaaattcgc ctacccgttc
    28381 acgcaccagc ggcacgcgag tagaaccgcc caccatcacc acttccagca cttcgtcagc
    28441 ttctacaccc gcgtctttaa gcgcgcgacg acaagccagt aaggttcgtt ttaccagtgg
    28501 cgcgatcagt tcattgaatt gttcacggct gatttcgccc tgccagcccg caacgttaac
    28561 ggtcacggag tccgcatcgc tcagcgcgat tttggctgca atggcggcat ccagcagttc
    28621 acgctgaacg cggttatcgc tacgatcagg aatgcccgcc tgctcgcgaa tgtaatccgc
    28681 cagcagatgg tcgaaatcat caccaccgag cgcggaatca ccgccggtcg ccagcacttc
    28741 aaacacaccg cgacttaagc gcagaatgga aatatcaaac gtcccgccgc cgaggtcata
    28801 aacggcgatc acgccttcct gaccggaatc cagcccgtag gcgatagccg cagcggtcgg
    28861 ttcgttaagt aagcgcagga cgtgaaggcc cgccagacgc gccgcgtctt tggtgccctg
    28921 acgctgggca tcgtcaaagt acgccggaac ggtgataact acaccatcca gctcgcctgc
    28981 cagggcttca gttgcccgcg ccgccagtgc tttgaggatg tccgcagaaa cgcgcaccgg
    29041 gttcagcagc cccgccgccg tttcaatcat cggcaggccg ttttcgctgg cctggaattg
    29101 ataaggcaga tgcggatagc gttgctggat atcagccagc gagcgcccca tcaggcgttt
    29161 aacagaacta atggtgttgg cggtgtcaag cgctgcgtta gtacgcgcgt cataacccac
    29221 cgaatgccct tgctgttgat agtgaacaac agatggcagc aggtgacggc cttcatggtc
    29281 ggctaacgtt tcggcctgac cgctgcgcac tgtcgccacc agcgagttgg ttgtgcccag
    29341 gtcaatacca gccgccagac gacgctgatg cggcgcagca ctcaaaccag gttcactaat
    29401 ttgtaataag gccatgttta gcttccagaa attaaaaatc gagcagtttt tcttcgagtt
    29461 gttcggcact gctccgcagt ttatcgagaa aacgcagctt acgcacggta tccgccgccg
    29521 cgtcccacgc ctcgttgtct aactgttcaa ccatcaactg atggcgggta tcaaacatct
    29581 ttttcacacg tttgataaag ctttccagcc gcgcttcatc tttcgcctgt tcgatctcgt
    29641 ccagctcttc gcgcagctcc aactgttcca tcaggaacgc ggtgtcgcgc acagtatgct
    29701 gctcgctggc gagatcaaaa ccgtgtaaag aaagcaaata ttccgcgcgc attaacggat
    29761 gacgcagcgt ttgccaggcc tggttaatgg ttgcagattg ctgtacggcg gcgagttgtt
    29821 ccgcctggct tccgctggcg aatttatcag gatgatactg acgttgtaga tcctgaaaac
    29881 gcaggctcag cgcctgggta tcgagttgat aacgggcagg caagccaaag agggtgaagt
    29941 aatccataac attctcaggg ctgcgatccg ccgcgataac ggcgaatcag gtaaaacaaa
    30001 ccccacgcgc aggcgaccac ggtggggtta tcggtatgcg catcaaacgt ggaagctttc
    30061 gccgcaacca cactcatctt taacgttcgg gttggtgaat ttaaaccctt cattcaggcc
    30121 ttcttttacg aagtccagct gcgtaccgtc cagaaattgc aggcttttgc catcgaccac
    30181 gactttcacg cctttgtctt caaacacgat gtcttccggc gtcggttcgt caacaaattc
    30241 cagtacataa gccatacctg aacatccgga ggttctcacg cccagacgca ggccaaaccc
    30301 tttgccgcgg ttagccagaa aggtatttac tcgcgctgct gcactgtcgc tcagtgtaat
    30361 cgacataacc aaacctcaac tcttattttg cttcacgttt gcttttatag tccgcaatgg
    30421 cggctttgat cgcgtcttct gccagaatag aacagtgaat tttcaccggc ggcagttcaa
    30481 gttcttcagc aatatcggta tttttgatcg cctgcgcttc gtcgagagac ttccctttca
    30541 cccattcggt gaccagggag ctggaagcga tagcagaacc gcagccgtaa gttttaaaac
    30601 gcgcgtcttc aatgatacct tcatcgttga ctttaatctg caacttcatc acgtcgccac
    30661 aggccggtgc acccaccatg ccgctgccga cgttctcgtc gttgttgtca aaggaaccca
    30721 cgttacgcgg attctcgtaa tggtcgataa ctttttcgct gtaagccatt ataaattctc
    30781 ctgattccga taccgattaa tgatgagccc attcgatgct gttcagatcc acgccctgct
    30841 tgtacatttc ccacagcgga gaaaggtcac gcagacgacc gatggattta cgaactaact
    30901 cgatggtgta gtcgatctct tcttcagtag taaaacgacc taaagagaaa cggatagagc
    30961 tatgtgccag ctcgtcgttc agccccagcg cgcgcagcac gtaggacggt tcgaggcttg
    31021 ctgacgtaca ggcggaacct gaagaaactg cgaggtcttt cagcgccata atcagcgact
    31081 caccttcaac gtagttgaag ctgacgttga gaatgttcgg cgcaccgtgt tccaggtcac
    31141 cgttcaggta aacttcttcg atatctttga tgccgttcca cagacggtta cgcaggccgc
    31201 gcagacgttc catctcggtc gccatctctt cttttgcgat gcgataggcc tcgcccattc
    31261 cgacgatctg gtgaacaggc agagtgccgg aacgcatacc gcgctcgtga ccgccgccgt
    31321 gcatttgcgc ttcgatgcgt acgcgcggtt tacgacgtac atacagcgca ccgatacctt
    31381 tcgggccata gattttgtga ccggagaaag acatcaggtc aactttcaac tggctcaggt
    31441 cgataggcag tttgcccacg ctctgggttg catcaacgtg atagataatg ccacgagcac
    31501 ggcacatttc gccgatagcc gcgatatcct gcaccacgcc gatttcgtta tttacgtgca
    31561 tgatggaaac aaggatggtg tcgtcacgca tcgctgcttc aagttctttc aggtcgataa
    31621 tgccgttacg ctgcggtgcc aggtaggtga cttcaaaacc ttcgcgctcc agctgacggc
    31681 aggtatccag taccgctttg tgttcggttt tgctggtgat gatgtgcttg ccttttttct
    31741 gataaaagtt ggctgcacct ttgatcgcca ggttgtcaga ttcggttgca ccagaggtaa
    31801 agacgatttc acgcggatca gcgccgacca gatcggcaat ctgattacgg gcgatatcta
    31861 ccgcttcttc agcctgccag ccgaaacggt gagaacgaga ggccgggtta ccaaaggttc
    31921 cgtccatcgt cataaactgc atcattttct cggcaacacg cgggtccacc ggcgtggttg
    31981 cggagtagtc gagataaatc ggtaatttca ttgctctata aactccgtac atcactcaat
    32041 gcaaggaacc aggctaccgg ctggatgtac gaccgtgttt acggagcgcg atacgccccg
    32101 gcctgattct aatatcttgt tatctttaac ctaactgttt cgggtttcag caagttgaca
    32161 aacttacgaa tctccgggag cttacataag taagtgaccg gagtgagtat gtgcagtcaa
    32221 cgcagatgca acacgaaaca ggacggttaa attaagcgcg taacttaacg tcgatcgcgt
    32281 cttgtatgcg ggtgcgtggc gcgtcgtgag tatgctgacg accagacaca tccagcactt
    32341 cctggttatt aaccagttcg cctaaagtaa tgttgttgag aaaaccggtg agacggtcgc
    32401 tcaaatcacg ccacagcgcg tgggtcaggc atttatcgcc gccctggcag ccgcctttac
    32461 cctgacaacg ggtggcatct acagattcgt caacggcgct aatcacttcg ccaacggcga
    32521 tgctgctggc atctttgcct aacagataac caccgcctgg tccacgtacg ctggaaacca
    32581 gaccattttt acgcagacgg gaaaacagtt gttccagata agaaagggaa attccctgac
    32641 gttcggaaat atcagccaac ggtaccgggc ccgcttcaga gttgagcgca acgtcaagca
    32701 ttgcggtcac ggcatagcgc cctttagatg tcagtctcat gtcttacttc acctcaaact
    32761 cgcccctgcc cggggttttt tattgtaaaa gtgggggtat tgcatagcag ggtcaagtct
    32821 gacattcccg agtaaattgg tcaactattt acttgactga tttagtcggg tatttaacct
    32881 tcagtgccat ttttttatcg tggcgtttgt acctgttgtc cttccagacc atgggcgggt
    32941 tgagggcaat tattcgcatt agcgctggcc tcttcccctc accctaaccc tctccccgaa
    33001 ggggcgaggg gaccgtccgt gcatttatct ggcttagccc tcaggcctta ttacctttat
    33061 tctgctgctc aatagaagcc agaatcccgc gcaggatatt caattcctgg ctttccgggc
    33121 gcgcacgggt aaacagacgg cgcaatttat tcatcacctg ccccggatgg ttttcacgga
    33181 taaaaccggt tgccagcagc gtttgttcca gatgaccata aaaacgctcc agatcatcga
    33241 ccagcggata cggcgtctct tcatgctcga cctgctcgcc gttttcctga gttgccagcc
    33301 aggccatgcg cacttcatag gcaataacct gagccgccat cgccaggttc agcgagctgt
    33361 attccgggtt agccgcaatc gcgacatgat aatggcattt ctgcaactct tcattggtca
    33421 agccgacgcg ctcgcgacca aacaccagcg ccaccggggt atttgccgct tcagcgacgc
    33481 ttttcaggcc gcattcgcgc gggtcgagca tcggccatgg cagcgtgcgg gagcgtgcgc
    33541 tggtgcccac caccaggcta caaccggcta acgcttcgtc gagcgtatcg acgatgtgag
    33601 cattaccaat cacatcgctg gcccctgctg ccagggcaat cgcctgggag tcgggtttca
    33661 ccagtggatt aaccagccac agattggtta atcccattgt tttcatggca cgggcaacag
    33721 aacccatatt gccggtgtgt gacgtctcca ccagcacaat tcgaatattt tgcagcattg
    33781 tctttcttca tctaaagatt attcacgcat cttatcataa aacgaagaca gatgccgatc
    33841 tcgctgctat actctgcgcc gttttcccgt tctttaacat ccagtgagag agaccgatgc
    33901 atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc gggtaattta attgccaaaa
    33961 actatgaaac cccggacgct gtagaagcga gccagaaagg cagtaacgat ttcgtgacca
    34021 acgtagataa agctgccgaa gcggtgatta tcgacacgat tcgtaaatct tacccacagc
    34081 acaccatcat caccgaagaa agcggtgaac ttgaaggtac tgatcaggat gttcaatggg
    34141 ttatcgatcc actggatggt actaccaact ttatcaaacg tctgccgcac ttcgcggtat
    34201 ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt ggtatacgat cctatgcgta
    34261 acgaactgtt caccgccact cgcggtcagg gcgcacagct gaacggctac cgtctgcgcg
    34321 gtagcaccgc tcgcgatctc gacggtacca ttctggcgac cggcttcccg ttcaaagcaa
    34381 aacagtacgc cactacctac atcaacatcg tcggcaaact gttcaacgaa tgtgcagact
    34441 tccgtcgtac cggttctgcg gcgctggatc tggcttacgt cgctgcgggt cgtgttgacg
    34501 gtttctttga aatcggtctg cgtccgtggg atttcgcggc aggcgagctg ctggttcgtg
    34561 aagcgggcgg catcgtcagc gacttcaccg gtggtcataa ctatatgctg accggtaaca
    34621 tcgttgctgg taacccacgt gttgtgaaag ccatgctggc gaacatgcgt gacgagttaa
    34681 gcgacgctct gaagcgttaa tgactcaggc gggtgatgtc actcacccgc cctcgccttt
    34741 caggcgctat tctgaaatac ttcctcaccg ctttactttc tttcgtcact ctcccaccat
    34801 ctttccccga ttaatggatg ataaagaagt cgcaaggaaa ggttatggca ctgccagtga
    34861 acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt tgccttctgc gtttatttag
    34921 tgccgcgcgt tgccatcaac ttcttctatt atcctgacga taaaatttac ggtcccgacc
    34981 cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg tactcgtctg caaggctggt
    35041 ttattccttc ttcgacgggc cctgctgaca acgccatcgc aaccatcatt catgctcacg
    35101 gcaatgccgg aaatatgtcc gcccactggc cgctggtcag ttggttaccc gagcgtaatt
    35161 tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc aaaaggcacg ccgtcccagg
    35221 ccggattgct ggacgatacg caaagcgcca tcaatgtggt gcgccatcgc agtgatgtaa
    35281 acccacaacg gctggtgctg ttcgggcaga gtattggcgg ggcgaatatt cttgctgtta
    35341 ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct cgactccaca tttgcctctt
    35401 atgcaaccat cgccaaccag atgatccccg gcagtggcta cttacttgat gagagttaca
    35461 gcggcgaaaa ttatatcgcc agcgtcagcc cgatcccgct tttactcatt cacggtaaag
    35521 ctgatcacgt tatcccatgg cagcacagcg aaaagttgta tagcctggca aaagagccaa
    35581 aacggttgat cctaatcccg gatggcgaac acattgatgc tttttccgat cgccacggcg
    35641 atgtttatcg cgaacagatg gtggacttta tccttagcgc gttgaatccg cagaactaac
    35701 ccatgatcgc tagcacgata atcattcaca aaaccacctt aagacatgct aatccactgg
    35761 tcagaacagt ttaagatgag aaaaattctg tgacgcttgc caacatttct gatgattagc
    35821 attcccttcg ccatttccct gagcaaactt tagctattct tatcaattat gcttatggga
    35881 gatctacaga tgatgcctac gcttgctcca ccatctgtcc tttcggctcc ccagcgccgc
    35941 tgccagatct tgctgacgct ctttcagccg gggttgaccg ccaccatggc aaccttcagc
    36001 gagcttaatg gtgtggatga tgatattgcc agtcttgata tcagcgaaac aggacgggag
    36061 atcctgcgct atcatcaact cacactgacg actggttatg acggtagcta ccgggttgaa
    36121 ggtacagtgc ttaaccaacg tttgtgttta tttcactggc tacgacgtgg tttccgtctg
    36181 tgtccgtcat ttattaccag ccaattcacc cccgccctga agagtgaact gaagcggcgc
    36241 ggaattgcgc gtaactttta cgacgatacc aatctacaag cgttagtgaa tctctgctcc
    36301 cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt tcctgtgtct gtatctgcaa
    36361 tattgtttgc tgcagcacca cgctggaatt acgcctcagt ttaatccgct ccaacgtcgc
    36421 tgggcggaat cctgccttga atttcaggta gcgcaggaaa ttggacgcca ctggcagcgt
    36481 cgggcgctcc agcctgtacc gcctgatgag ccactgttta tggcactact tttttccatg
    36541 ttgcgggttc ccgatccact gcgggatgcg catcagcgag acagacaatt gcgtcagtct
    36601 atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg ttcgttttta tgatgaacag
    36661 gggttatgtg atcagcttta tacccacctc gcccaggcgt taaatcgcag tttgtttgcc
    36721 atcggtattg ataataccct gccggaagag ttcgccagac tgtacccacg cctggtgcgc
    36781 accacccgcg cggcgctggc cggatttgaa agtgaatacg gcgtccatct ttctgatgag
    36841 gaaagtggtc tggtcgcggt gattttcggt gcctggctaa tgcaggaaaa cgatctgcat
    36901 gaaaaacaga ttattctact gaccgggaat gatagcgagc gagaagcgca gattgagcag
    36961 cagttacgcg aactaacgtt actgccgctc aacattaagc atatgtcggt aaaggcattt
    37021 ttgcagacag gcgctccgcg tggcgcggca ctgattattg cgccttatac catgccatta
    37081 ccgctctttt caccaccgct gatctatacg gacctgacgc tgacaacaca tcaacaggag
    37141 cagatccgca aaatgctcga atcagcatga gggaacaact ttcgggcgca aaaacattgc
    37201 cggcagcgcc accagcgcca ttacccagaa cacgccgtgg cccagatatt gatacaggaa
    37261 accggcgaaa acggtcatga tagcgatact gccgcccatc gcgacggcag agtaaaccgc
    37321 ctgtaaacgg atgacttcgc taccctggcg agcagcaata taacgcatgg cggccaggtg
    37381 gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact atcaaccacg gcaacgccgt
    37441 agttgctccc ataatgcccc agcgcactac gccgcaaatc gccgagatca acagcatatc
    37501 gcgtgcacta caacggcgga aaagtttatt actcagcgca aagataatga cttccgccac
    37561 cacgcccagc gaccacaaat accccaccgc cgaggccgag tagccagctg cctgccagta
    37621 aatggcgcta aaaccgtaat aggccgcatg tgccccctgc aataaacaaa cgcaggccag
    37681 aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac caaccggtgc tctcctgctg
    37741 gcgacttgcc ccttgtggct gaatcgtcgg acggatgaga aagccgagca gcatggatgc
    37801 cacgcccaac gtcaacagcg cgaggatcac ccgataatca aacatagtga ccagtttgcc
    37861 cgtcagcgcc gagccaatga caaacgccac cgagccccac agtcgcactt tgccgtaatc
    37921 aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg gtcaacggta ccagtggtga
    37981 gaaaaagagg ttaaagccaa tcatcaccag catcagccac gctacgtgcg cccccgccca
    38041 gaaggcgaca gcaaagagaa gtgtcagcag tgccagcacg cgcaaggcgg aaatcaggcg
    38101 ggaaggatcg ctgacgcggg gcgcgatgag caaactcccg aggaaacggg caaccagacc
    38161 tgcccccaat aacaggccga tggtttctgg cgttaaacca atccctttaa gccagacgct
    38221 ccagaaaggt agaaaaatgc cgtaactaaa aaagtatgtg aaatagccga gcgccaacca
    38281 gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag gcgttagtct ggggttaatc
    38341 gaatgttggt gcaagttgaa aaataagaat attcgagcct gcttcccgat gtctgtaacg
    38401 ccagttcaca agcactaaaa aactgagatc cgcgacgcat gttgcaaaaa taatgcgtaa
    38461 ttatgccgtt acgcttgcca aacgttcctg cacaatggcg atgaaatcgc gtaatgccgg
    38521 cttcatctcc cctttcttcc acgccatcag taaagcaatg gaaggcacat ttccggcaat
    38581 gggacgaaaa acaacctgtc cggtattaaa attattcata taaccgggta tcaaagtgac
    38641 gcccagcccc atgcccacca gattcatggt caccagaata ttcgttgcca cctggacgat
    38701 atttggctgg ctgttttctt gcgcaaacca cgctttaacg atcggcgcaa gcgaaccgga
    38761 atacaccgga tcggtactga cgaaattcac gccatccagt tgcgcggcgg tgatctcttt
    38821 ttcatgtgct aaagggtgat caaccggtaa cacaaccact aatggttcgt caaaaagctc
    38881 cagataatca atctccgggc tataaacagg atggcgcatt aagccgacgt cgagttcacc
    38941 acggcgaatt ttttcctcct gttgcgtggt gattaaactc accagctcaa tcaaggtgtc
    39001 tggctgtctg agacgaaaca tcggtaatac ttttggcagt aaattcactt ccgccgatgg
    39061 cacaaagcca atggttaatt gtctgtcttc ctgaacaatt ttccgcgccc gtaatttggc
    39121 attttccgct tgttcgagga ttgccagcgc gtcctggaga aaacattctc ccgccgccgt
    39181 caacgcgact ttgcgcttat ccctcaccag taacggaaca ccgacacagt tttcaagatc
    39241 gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt ttttccgccg cacgggtaaa
    39301 gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc cgtagttcca tcaccttccc
    39361 cttgttatcg aaaaaacgtc tcagctggta gaaattaact atttcacata ttagcaacca
    39421 accagcaaca tccttatggc acaaaaatag aaggtcaata catcttatct ttcaggatta
    39481 aaaaatatga ccacaccctc agatttgaac atttaccaac tgattgatac ccaaaatggt
    39541 cgggtcactc cgcgtattta taccgaccca gatatttacc aactggagct tgaacgtatt
    39601 ttcggccgtt gctggttatt tctcgcccac gaaagccaga tcccaaaacc tggtgatttc
    39661 tttaacacct acatggggga agatgcggtt gtcgtagtgc gtcagaaaga cggcagcatt
    39721 aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg tgagttatgc agattgcggc
    39781 aacactcgcg cctttacctg tccgtatcac ggctggtctt atggcattaa cggcgagttg
    39841 atcgatgtac cgctggaacc tcgcgcctac ccacaagggt tgtgtaaatc ccactgggga
    39901 ctaaacgaag ttccttgtgt ggagagttat aaagggctga tttttggcaa ctgggatacc
    39961 agcgcaccgg gcctgcgtga ttacctgggt gacattgcct ggtatctgga tggcatgctg
    40021 gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac aaaagtgggt gatcaactgt
    40081 aactggaaat tcccggcaga gcagttcgcc agtgaccagt atcatgctct gttcagccat
    40141 gcttctgccg ttcaggtatt aggggcgaaa gatgatggca gcgataagcg cctcggtgat
    40201 ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg cgctgcaatt tggtcaggac
    40261 ggtcacggta gcggtttctt ctttactgaa aaaccggatg ctaatgtctg ggtcgatggc
    40321 gcagtttcaa gctattaccg cgaaacctat gccgaagcag aacaacgttt aggtgaagtt
    40381 cgcgccctgc gcctggcggg tcataacaat atattcccca cgctttcatg gctaaacggc
    40441 actgccacac tccgcgtctg gcatccgcgc ggccctgatc aagttgaagt gtgggcgttc
    40501 tgtattactg acaaagccgc ctccgatgaa gttaaagccg cttttgaaaa cagcgccact
    40561 cgtgcttttg gtcctgctgg ttttctcgag caggatgact cggagaactg gtgtgaaatc
    40621 cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac tgtgtctgga aatggggctt
    40681 ggtcaggaaa agcgccgcga cgacggcatt cctggcatta ctaactatat cttttcagaa
    40741 actgccgctc gtggaatgta ccaacgctgg gctgatcttc tgagtagcga aagctggcag
    40801 gaagtgctcg ataaaaccgc cgcttaccag caggaggtga tgaaatgagt gcgcaagttt
    40861 cactagagtt acatcaccgc attagccagt ttctctttca cgaagccagc ttactggacg
    40921 actggaaatt tcgtgactgg ctggcgcagc tcgacgaaga gattcgttac accatgcgca
    40981 ccacagttaa cgcgcaaaca cgcgaccgcc gcaaaggcgt ccagccaccg acaacctgga
    41041 tttttaatga caccaaagac cagctggagc ggcgaatcgc ccgtctggaa acgggcatgg
    41101 cctgggcaga agagccgccg tcacgcaccc gtcacttaat cagcaactgc cagataagcg
    41161 aaaccgacat cccaaacgta tttgctgtgc gggtaaatta tctgctttat cgggcacaaa
    41221 aagagcgcga tgaaacattc tatgttggaa cgcgtttcga caaagttcgc cgtctggaag
    41281 atgacaactg gcgcttgctg gaacgggata tcgtcctgga tcaagcggta atcacttccc
    41341 ataacctgag tgtactgttc tgatgaatcg aatttatgcg tgtcccgttg cggatgtgcc
    41401 ggagggtgag gctctccgga tcgatacctc gcccgtcatc gccctgttca acgttggcgg
    41461 cgagttttat gccattaacg atcgttgcag ccatggtaat gcgtcaatgt cagaagggta
    41521 tctggaagat gacgccacgg tggagtgccc gctacacgcc gccagttttt gcctgaaaac
    41581 ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc acttatccag tacacgttga
    41641 aggtggtgac attttcatcg acttaccgga ggcgcagcca tgagcgatct gcataacgag
    41701 tccattttta ttaccggcgg aggatcggga ttagggctgg cgctggtcga gcgatttatc
    41761 gaagaaggcg cgcaggttgc cacgctggaa ctgtcggcgg caaaagtcgc cagtctgcgt
    41821 cagcgatttg gcgaacatat tctggcggtg gaaggtaacg tgacctgtta tgccgattat
    41881 caacgcgcgg tcgatcagat cctgactcgt tccggcaagc tggattgttt tatcggcaat
    41941 gcaggcatct gggatcacaa tgcctcactg gttaatactc ccgcagagac gctcgaaacc
    42001 ggcttccacg agctgtttaa cgtcaatgtt ctcggttacc tgctgggcgc aaaagcctgc
    42061 gctccggcgt taatcgccag tgaaggcagc atgattttca cactgtcaaa tgccgcctgg
    42121 tatcctggcg gcggtggccc gctgtacacc gccagtaaac atgccgcaac cggacttatt
    42181 cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga atggcgtcgg cccgtgtggt
    42241 atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa gtgaaacctc gataatgcag
    42301 tctctgacgc cggagaaaat tgccgccatt ttaccgctgc aatttttccc gcaaccggcg
    42361 gattttacgg ggccgtatgt gatgttggca tcgcggcgca ataatcgcgc attaagcggt
    42421 gtgatgatca acgctgatgc gggtttagcg attcgcggca ttcgccacgt agcggctggg
    42481 ctggatcttt aaggaagcac gatgaaagaa aaaaccatca ttattgtcgg tggcgggcaa
    42541 gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt tcaccggtga gctgcatctg
    42601 ttttccgatg agcgacatct tccttatgaa cgacctccac tctcgaaatc catgttgctg
    42661 gaagattccc cgcagttaca gcaggtgtta cccgctaact ggtggcagga aaacaatgtt
    42721 catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg acacacgaga gttagtgtta
    42781 accaacggcg aaagctggca ctgggatcag ctttttatag caaccggcgc ggcagctcga
    42841 ccgctgccgt tgcttgatgc actgggagaa cgctgcttta ccctacgcca tgccggtgat
    42901 gccgccagac tgcgagaagt tctgcagccc gaacggtcag tcgtgattgt cggtgccgga
    42961 actattggtc tggaactggc tgccagcgcc acgcagcgca gatgtaaggt gacagtgatt
    43021 gaactggcgg caaccgtcat gggccgtaat gcaccaccgc ccgtgcaacg ctatctttta
    43081 cagcgccacc agcaggctgg tgtgcgcatt ctgctcaata atgccattga acatgtggtc
    43141 gatggtgaaa aagtagaact gacgctgcaa agtggggaaa cgcttcaggc tgatgtggtg
    43201 atttacggta ttggtatcag cgccaacgag caactggctc gcgaggccaa ccttgatact
    43261 gccaatggca ttgtcattga tgaggcttgc cgcacctgcg atcccgcgat ctttgccggt
    43321 ggcgatgtgg caatcactcg tcttgataat ggtgcactac accgctgcga aagctgggaa
    43381 aacgccaata accaggcgca aattgccgct gccgcaatgt tagggctacc gctaccgcta
    43441 ctgccgccgc cgtggttctg gagcgatcag tacagtgata acttacagtt tattggcgat
    43501 atgcgtggcg atgactggct ttgtcgtggc aacccggaaa ctcagaaggc gatttggttt
    43561 aatctgcaaa acggcgtgct tatcggtgcg gtcacgctga atcaggggcg tgagattcgc
    43621 ccaattcgca aatggatcca gagcggcaaa acgtttgatg cgaaactgct gatagatgag
    43681 aacatcgcgc ttaaatcact gtaaccagga taattagcga atatctcaat gcctggggcg
    43741 tggcgaggtg caagagtgtg tattacgttt aaatcacatt atcttgcaaa gggattggtt
    43801 atgaacacac tacgttattt tgattttgga gctgcccgcc ccgttttgtt attaattgcc
    43861 cgtatcgccg tggtcttaat tttcattatt tttggttttc ccaaaatgat gggctttgac
    43921 ggtacggtcc aatatatggc ctcgttgggc gcgccaatgc cgatgctggc agcgattatt
    43981 gcggtagtta tggaagtgcc cgccgcgata ttaatcgtgc ttggcttttt cacccgtccg
    44041 ctggcggtgc tgtttatttt ctacacgctg ggtacggcgg tgattggtca ccattactgg
    44101 gatatgaccg gcgatgcggt tgggccaaat atgattaatt tctggaagaa tgtcagtatc
    44161 gctggcgcat tcctgctgct ggcaattacc gggccggggg caatttctct cgatcggcgt
    44221 taggaaagat gccggatgcg gcgtgaacgc cttatccggc atttaataaa ttacagccac
    44281 tctacccgca acgacatctc tgaagttgtt gattcccctg gtgctagcgc aatgagatca
    44341 cccccttccg ggcgatgatg atcatccggc gcatggctca tcggttccag acagaagaaa
    44401 tcaaacgcat atcctttatc aaacgcaggg tcggaaacaa agatgaaata acacggtgca
    44461 ggtggcgtcg tttccatgat gatggcataa ccttcctgcg gctgctcgat gcgggcctga
    44521 ccattccatc cggcaaaacc attgttcacc cactggcgcg gcaacggcgc gggctggtta
    44581 aaatccagtt cctgcggtag ctgctcgcaa aactcacccg ccagccactg ctcccgctcc
    44641 agccagtaac cgctcgcctg tgcctgaatc cgcgtttgcg gcgacaacgg aaaataagga
    44701 tgccagccgg taccaaatgg cagcgtctct gccccttgat tggtgacaga gagcgtcacc
    44761 gtcagcgtat ccgccgttaa atgaaacgcc tgacttaccc gatagtgata gacaccgctg
    44821 cgatgttcat acaccagaca caaactatca tcgctgtgcg agacacattg ccactcgccc
    44881 agccagccat cgccgtgtag atagtgcgca tcccactcaa cattcggttg cagttgatac
    44941 tcacgcccct gccagacaaa acgattgccg ctcacccggt ttgcaaatgg caccagcgga
    45001 aaacatgagg catcggttgc cacaccgctt tttttaccag gacgtaagag cggcgtcgta
    45061 tcgcgccaga agccttcgat tacgccgccc tggtcagaaa cgtccagctt tagcgacccg
    45121 tgggataagg tatagatggt catccgctaa ctccttaatc cgggaagtta atcacaactt
    45181 tcccgcattt gccgctcgcc atcagcgcat aggcatcacc tgcctgttcc agcgagaagc
    45241 gatgggtaat ggcgttacgc ggccacagtt tccagtccgt cagatcatgg gcgcattttt
    45301 ccatatggaa cagactggtc acccaggagc cgataatccg ccgttgatgg tgcatcagat
    45361 cggcgctgac ctcgaactcc acttttccgg tttctccgat gtaaaccacc cgtccccagt
    45421 cagcggtgga tcgcagtgcc agcaagcgac ctgcggcatt accagaacaa tcgagcgcaa
    45481 catccgcgcc accgtgggtg agttcggcga taatctgcgg cagaccttcg gtggttgcta
    45541 aatagccgtg atccatcacc cctaactgtt ttgccatcgc cagacgttcc ggcagcatat
    45601 caacgccgat gatccgtttt gcaccgcgac ctttcgccag catcatcgcc atcatgccga
    45661 ctggccccag accgaccacc agcacgttat cactgccgga aacttcgccg cgcaaaattc
    45721 cttcatacgc tgtaccaacg ccgcaactga taaacgcacc atcttcgtag ctcagcgcat
    45781 ccggcaggag gatcagatct ttttcttccg ccagcagata ttcggcatga ccgccgtcac
    45841 gctgccagcc gtaagccgct tttcctttgc cagtacaaga gataggaaag ccgcggcggc
    45901 agttcgggca aaaaccacag ccagaaatgt gatacaccag cacgcggtcg ccctctttaa
    45961 aatggcggca gccttgcccc atcgccacaa tctgcccgca cggttcatga ccgttgataa
    46021 atccctggta taacggttta tcgggtgccg ccgccgtggc acggtgttga tgatagatat
    46081 agtggacatc gcttccgcaa atcccggagg atttcatttt gatcagtacc tggttaatcc
    46141 ccggcgtcgg caccgcaact tcccgcagat cgacggttga atttcctggt aaataagctg
    46201 ccagcatcgt tttcataaat ccctcattaa caatacgatt aattttcatc cctgccgcac
    46261 ccgcgccagg ggcgttaagg tttagcgttt cgctttactg cttcgctggg tcagcaagat
    46321 attcgccagc accgccacca cgatgatgac gccgcgtacc acctgctgga aaaaggagtt
    46381 aataccgagc agcaccagac cgttaccgat tagcgtaatc accagcacac caagcaatgt
    46441 accgaacagg gaaccgcgac cgccggaaag tgccgtaccg ccgaccacga ccgcggcgat
    46501 gacgtcaaac tccagaccgt ttgcggcacc tgcgttaccg gaaccgaggc gtgccgccag
    46561 caaaatgccg gtcaccgccg ctaataatcc cgaaagggta aagataagaa tgcgcactcg
    46621 acgaacgtta ataccgcaca actgcgccgc cgtggcatta ccgccaacgg caaaaaccga
    46681 gcgcccgaag gcggtttttc ggctaatgaa cacaaacagc gcaaacaaca ccatcatgat
    46741 cagcgcggat actggcacac cgagaaattg tccgcccagc cagtccagca cctcgttttc
    46801 gtcaatcggc actggcagtg cgttggtcat aaacagcccc attccgcgca gggcgctcca
    46861 cagccccagt gtggcaacga aacttggtac gttaaacacg ccgcgcagca ccccggcgag
    46921 cgttcccatc agcgcgccta acagcaacac cagcagacac gccaccgcca gcggaacttc
    46981 aaattgcagc aaaaatgcca ggcacaccga gacaaaagcc accatcggcc caacgctgac
    47041 atcaatttca ccggagataa taatcagcgt catcgcccag gcggcaatcc caatggtggc
    47101 ggcatcgcgc agcacgttca tctggttatt caatgagata aagcctggcg cgttcaggga
    47161 gaagaccaga taaagaatgg caatcaccac cagcaaaccg atctcattaa tatggcgact
    47221 gacaaattgt ttgagcgaga cgctcttgcc ctgcggcaat ggtaatgacg aagcagacat
    47281 cttcaggttc ctcggtaaaa ttcatcagtg cacagacaga atggcggaca tcagctcatc
    47341 cacattgacc ggagcgtgaa actcctgcga gaacgtgccg tgctgtagta acaggatgcg
    47401 gtcacagacc agcggcaact cctccacttc actggagata aacaccacgc tttttccttc
    47461 ggcagccagc tcacggacaa tacggtaaat ctgctgtttg gcttcgatat cgacgccgcg
    47521 cgttggctcg tcgagcaaca aaatctggct ggcggcatag acccaacgac cgatcaccac
    47581 tttttgctga ttgccgccag aaagcgtgcc gatgggcgtt tcgctactgg cggccttgac
    47641 cgtcatccgc tgcatcacct cttcggtcag gcggcggatg gtggaccatt gcagtacgcc
    47701 gttggcgctg attttttgcc gattggtcag cactgtattt tcgtcaacgc ccaaccaggg
    47761 aatgatcccc gcttctttgc ggttttctgg cgtatagcca atgccgcgtt tcagcatgtc
    47821 gccgtaatcg gggcgcgtga ttttctcgcc gttgataaca atttcgccct gttcatactc
    47881 ctccagccca acaatcgcct tcagcaattc actgcgccct gcccccagca ggccagcaat
    47941 gccaagcact tcgccgcgac gtaacgtaaa gctgatatcc tccagcttgg gcttatggcg
    48001 taatgcacgg acttccagca cggcctgatc cacaatttcc tgaggggcaa ccggcgcaat
    48061 atcaacgtga tcacgcccta gcattagcga cacaatatga tgcgtggagg tgttttcgag
    48121 catcacatcg cccgccacct gaccatcgcg cataacggtg gcacaggagg caatgcggcg
    48181 aatttcttcc atccggtggc tgacataaat caccgccacg cccagtgccg acatcttttt
    48241 caccgcgatg atcaccagtt caacttccgc actcgcaagc gaactggtag gttcatcaag
    48301 aatgaccacg cgcggctcgc ccttcatcac ccgcgcaatt tccaccagtt gcttttgcgc
    48361 cgggcttagc gttgaaacaa gttgttcagg gctaacgtca acgcccagcg cctgtaagca
    48421 acgttgggca tcctgcgcca tttgcaggta atcaatcatg ccgttgcggc ggggccactg
    48481 accgaggcag aggttttccg ccaccgtcag tccctccacc agacttaact cctgataaac
    48541 cgcacgaacc cccagttcag cggcacggcg agtcaacgta gcttcgtcac cttccagtcg
    48601 cgtctcgcca atccagatat caccgctatc cgggcgttcg ctgccggtaa gcattcgaat
    48661 gagagtcgat ttgcccgcgc cgtttttacc taacagcgca cgaacttcgc ctttattgag
    48721 cgtgaagtta acgttatcca gcgcaacgac gccggggtaa cgcttatttc ctgccaccac
    48781 ttttgctacc gggactgcct ctgttgccgt gaacataggg acctctgcga atcagcgatt
    48841 agggcagacc atcaacgtgc gttgccagcc actgtttacc atcttccgtt ttggtataga
    48901 gatcgattgg cacctgaatc actttttcac cgtcggcttg tttattgata accttcaatg
    48961 tttgcgcgaa aacagcattg cccattttct taccggaaat atccactacc gctttcagca
    49021 cctgattgtt ttccagctcc tgagcaattt cggttgtcat atccgaaccg aaaacagcaa
    49081 tttttccggc ctgattttga ttacgtaccg cttttaccgc gccgagtgtc gcaccgcccg
    49141 actcccccat aatggcgttg agatccggcg tggagataat cagtttttca ccaacggaaa
    49201 tcgctttatc taaaacagtc ccttcctgat tagcgacaat ttgcgcgccg ggaacgcggg
    49261 attttaatac ttcttcaaat cctttacgtc gctgcacaca aacttcaaag gcttcgcaat
    49321 tgatgacagc aattttcggc tggtcaattt tattggcaat aaaataatcg gcggcagcgt
    49381 tacccagttt tttaccaaat tccagcggat cgccgaccag atacgccgag acatatttat
    49441 cgcccccctt ttgattaata caggtgttgt agcaaatcac cggaataccc gcttcactgg
    49501 cgcgacgaac ggtacggcta ctgccatttt cagacactgc cgataaaata atggcatcga
    49561 cattacgcgc cacgagggta tcaacaaagg tactttcttt cgaaatatca ccctgggcgt
    49621 tagtttcaat taactgtacc tgtactgaag aatcttttgc cgcatcctga acgccctgac
    49681 gcactccagc gtaatatccc tgggtatcaa ggtatattgc accaatggtc atttctttat
    49741 ccgcagccct ggcaaatagt gcgctaccta atagcgtagc cattaataat agattacggg
    49801 ttgttctcat ttttttaggc atagtgcctc ctgtagggtt tttattaaca acggcttatt
    49861 ctaattattt tgtgatgagc ggcagcgcgt gccgctcaaa tattacagtt aatagggttt
    49921 agtgaacgta gttaaaaata aaaggcatca cggtggtgaa taatgccgct ttcggccagg
    49981 ccggattaat cgccgtcaat tcagcgcgag cagattcata ttgcgcaacc tccccgagtc
    50041 ccgcggacgc cagcatcgcc accatcaggc atttttcttt atgctgctgt tgtaaatcgc
    50101 catacagcga caacaggtca ggttgtgaaa cagcaaagaa atccgcctca atactggttt
    50161 tcgccatctc ttgcgcccac tgtttcattt cactaaacag ttgctgtgcg gtttgctgtt
    50221 cacccagcag tcgcagcgcc attccttgcc agaagagata atcaaccggc tgatcgttgt
    50281 aataactgtg gatgttaatg gtgcgatcgc cggtcgccgc cagacgtaaa caacgcatcg
    50341 cttcagtttc atcgccctgc gcgttggcgc atatcgcctg ccagaaccag atgtcgttat
    50401 cagtttgccc cggtaaacgg ccttcgctta aattctccgg ataatgcagc gcggcatgaa
    50461 gcagttcgct ggcctgctgc ggctctctgg catcaagatg ctgccaggcg cgtaataact
    50521 ggttgaggat aaactgactg gtgaccttcc cttccccgcc ttcccacggg tggaatttac
    50581 gcgtggcgag aatgtccgcc gctttgtctg cctgacccgt gagatgccac aaattgagca
    50641 gttctgcggt catgtcgtcg cgtttcagcg caatttcctg attattttcc agacgcgcca
    50701 gtcgtttctc cggtgtggct ccacttaact tatcaagcaa atcccgttcg aaaagcagac
    50761 gtgcatcctg cggcgcaagc tgataagcat tatcaagata acgcgcggcc agctcataat
    50821 cgtgttgctt attccacgca tggatcgcta acccgcgcca gccgtcggca aactccggcg
    50881 acatctctac gcaacgttgc cataaggcaa tggctttgtt gtagctacgt ttgttgtagt
    50941 agaagcaggc cagtaaatgg cgagcaaacc agcactcttc aatactctcc agcgccgcca
    51001 cttcttccag cgtattcggg aaacggacaa actgcgggaa gacatcaatg gcttttgcga
    51061 ccagttcgcc acgttcggct ttcggcagca agctggcttg caggtaaagc ggcagcgtgc
    51121 gctggcagtc cagtgcgttc agcatctctg ccgccagggt gggcattccc cagttaatca
    51181 gttgcccggc ggtcatcagg gcgttaacgt cgcgtccctg acacagaccg cgccactgcg
    51241 cgagagctga ttcgctacga ccatcgaacc agttcagcca ccacagagtg gcgttcagcg
    51301 gataatcgcg cagcagtttc tcgcgctgca cacgcgcgtt gtcctgacga ccacttaaca
    51361 ccagcagcag attatgcagg caaagcactt cctgattggt tgggcaggcg cgaagacttt
    51421 gttggcaaaa atccagacca gcgtcgaagt taccattacg cgccgccagt cgtgccaggc
    51481 cataatagcc accggctttg ctgttgccgc tccagaccgc acgccagaaa tcctcttcgg
    51541 cttgttgata ttgtccctga cgttcgtaag cactggcgcg aatcaaactc gcctgtccgc
    51601 actgcggatt tttgttcagc gcatgtgcgc gtttcagagc ctgagtggca tacgccaccg
    51661 cttgcgggaa atcggcgcgg ttatattcca gcatcgccag cgccaggtta cagcggtaat
    51721 ccagcggatc cagcgccacg ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat
    51781 gatattgctc cagatgctga ccgataaacc aggcttcatc tgtactggta atgtcttgtg
    51841 ctgccagtgg cgctttggcg acgtccggca acggcaactc ttgcggctga tgttcctgat
    51901 aactcagtac aatattgccg tcggcatcgg agagctcaat ggtcagcctt tcaggattga
    51961 taccgtgcaa cacgccctgg atggcggtcg caggcatcag tgccacggca tcatcaagta
    52021 acgcgttgca tttgccgatt tcgcggatcg ccaggcggta tccgttcaac ggagagatgg
    52081 catacagccc ccactcaatc ccccgcttac tacgctggag ttttatcacc gcatcgcggg
    52141 aggcattttg caccatgccc aaagaatgat aaggcaggaa atactgctca aaacgcttct
    52201 cttcgtaagc atcaagccag gtaaaatcgg gctggttatc ggcaaaaata ccggtcatca
    52261 gttcgatata cggaccgtta ttgtcggtca gactcttatc ccacgcctgg ccaaattcac
    52321 tgtgtcccca actccactgt tttttacctg gcgcaatatg gtggttggca acgtgcagca
    52381 aaccgccatc ttcatcgtga caccacgcgc cgacaaaatc gtactgcgat ttttcggcca
    52441 tatatgaggt tggaacaggc acatttttat agcgagaaat gtccactccg gcggagtagt
    52501 ccactttgta gtaagtgccg gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat
    52561 caaacaccgc agtcacatcc ggtgggaaaa cgctctgatg cccttccccc cctttcactg
    52621 ccgggttggc ccaccacaag aaatgacgcg gcgtggcgtt accgttatag acgcggctgg
    52681 cgatttccag cgccgcccgg tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat
    52741 gcatcggctc cgtttcgcct acccacaccg tctgtgcacc gtcttcatgg gcttcgaggg
    52801 tgaaatcaac gggcataaag gtggtcgggc gatgatgttg cggccagtta aactcaatcc
    52861 caccggaaat ccacggcccc agcagcccca ccagcgcagg tttaatgact tcattgtgat
    52921 aaacaaaatc gcgctgtttc actttatccc acgcgcgatg cacccgaccg cccagttccg
    52981 gcaggatcat cactttgatg tagtcgtttt ccagccacac cgcctgccag gatttcaggg
    53041 ttttctgctc gctcagcgta tcggtcacgc cgtagggata aaccgcgccg gacgatccct
    53101 gataaacgcg attttcgagg aacatgggat gtatatcctg cggcccggtt tcataggtcg
    53161 ggatctcaac gcgctcttgc cacactttta ctggagtcat ggtgccctca atattaacaa
    53221 gacatactga attaaaagat ttgtggcagt gtattgaaca atctggcaat gttttcgcgg
    53281 aataatcacg caattaacta aacaaggttt agtgaagatg agagcctgca ttaacaatca
    53341 acagattcgc caccataaca aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc
    53401 caataaatca acgctggccc ggctggcgca aatttcgatt ccggcagtca gtaatatttt
    53461 gcaggaactg gaaagcgaaa aacgggtggt gaatattgac gatgaaagcc agacgcgcgg
    53521 gcatagtagc ggtacatggc tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt
    53581 gacgcccacc agtattgagt gtcaggttgc taatgcttgt ttaagtccga aaggtgaatt
    53641 tgagtattta cagattgatg caccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg
    53701 ctggcatcgc caccgtaaat tgtggccgga ccgcaccatc aatctggcgc tggcaatcca
    53761 cggtcaggtt gatcctgtga ccggcgtgtc gcaaaccatg ccgcaagcgc cgtggacaac
    53821 gccggtggag gtgaaatatc tgctggaaga gaagctcggc attcgggtga tggtcgataa
    53881 tgactgcgtg atgctggcgc tggcggagaa atggcaaaat aattcgcagg aacgggattt
    53941 ctgcgtgatc aacgttgatt acggcattgg ctcgtcgttc gtgattaacg agcaaattta
    54001 tcgcggcagt ttgtatggta gcggacagat tggtcacacc atcgttaatc cggatggcgt
    54061 cgtctgcgac tgtggacgtt atggctgcct ggaaaccgtc gcctcgttaa gcgcattaaa
    54121 aaaacaggcg cgggtatggc taaaatcaca accggttagt actcaacttg atcctgaaaa
    54181 actgactaca gcgcagttaa tcgctgcctg gcaaagtgga gaaccgtgga tcaccagctg
    54241 ggttgaccgc agtgccaatg ccattggttt gagtctgtat aacttcctca acatcctcaa
    54301 tattaatcag atttggttgt acggtcgcag ttgtgccttt ggtgagaact ggcttaatac
    54361 tattattcgc cagacaggat ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc
    54421 gacgcaaatt ggcttcgggc aattaagccg cgcacaacag gtgctgggaa ttggctattt
    54481 gtatgttgag gcgcagttac ggcagatttg atggcgcaat aacgtagaaa ggcttcccga
    54541 aggaagcctt ttttcaacag attaaccgtt tcgcttatgc gtaaaccgga taacgtgcgc
    54601 agatgtcgag aactttacct ttgatgcgct cgataacagc ttcatcattg atgctgtcca
    54661 gcacgtcaca catccagcca gccagttctt tcgcttctgc ttctttgaag ccgcgacgcg
    54721 taattgccgg agtacccacg cgaataccgg aggtcacaaa cgggctcttc ggatcgttcg
    54781 gtacgctgtt tttgttgacg gtgatgttag cacggcccag agcggcgtct gcttctttac
    54841 cggtcaggtt tttatcaacc agatcaacca ggaacaggtg gttatcagtg ccgccggaaa
    54901 ccactttgta gccgcgctcg aggaacactt ctaccatcgc tttagcgttt ttcgcgacct
    54961 gctgctggta agttttgaac tcaggctcca tcgcttcttt cagagcaacc gctttaccgg
    55021 cgattacgtg catcaacgga ccgccctgac caccagggaa aacggcagag ttcagttttt
    55081 tgtacagctc ttcgctacca cctttcgcca ggatcaggcc gccgcgcgga cccgccaggg
    55141 ttttgtgagt ggtggtagta acaacgtgag catgaggaac cgggttcggg tagacgccag
    55201 cagcaaccag gcccgcaacg tgcgccatat caacgaacag gtaagcaccg atgctgtcag
    55261 cgatttcacg cattttcgcc cagtccacca cgccggaata tgcagagaaa ccaccgataa
    55321 tcattttcgg cttgtgttct ttggcttgtt tttccagatc ggcgtagtcg atatgaccgg
    55381 tagcatcgat accgtaagga acgatgttgt acagtttacc ggagaagtta accggagaac
    55441 cgtgagtcag gtgaccgcca tgcgccaggt tcatacccag aacggtatca cctggttcca
    55501 gcagcgcggt gtagaccgca aagttagcct gggagccgga gtgcggctgg acgttagcgt
    55561 agtcagcgcc gaacagttct ttcgcacgat cgatcgccag ttgttcaacg atatcaacat
    55621 actcgcaacc gccgtagtag cgtttgcccg gataaccttc agcatatttg ttggtcagct
    55681 gagaaccctg cgcctgcatt acgcgcgggc tggtgtagtt ttcggaggcg atcagttcga
    55741 tgtgctcttc ctgacgtact ttttcctgct ccatagcctg ccacagttcg gcatcataat
    55801 cggcaatgtt catttcacgc tttaacatcc gcatctcctg actcagctaa caataaaatt
    55861 tttggccttt ataggcggtc ctgttggaca acggcgaaca gtataaccga atcattgtgc
    55921 gataacaggt cttgacaaag gaatttacgc aaacgattac cttcaggcta cgcaaggctt
    55981 tggagaataa agagcttgca accggaaacg gatttctttt caggtttgtg atgcaaattt
    56041 ttcacttcat cacattcttt ctgaaaaaca ccaaagaacc atttacattg cagggctatt
    56101 ttttataaga tgcatttgag atacatcaat taagatgcaa aaaaaggaag accatatgct
    56161 tgacgctcaa accatcgcta cagtaaaagc caccatccct ttactggtgg aaacggggcc
    56221 aaagttaacc gcccatttct acgaccgtat gtttactcat aacccagaac tcaaagaaat
    56281 ttttaacatg agtaaccagc gtaatggcga tcaacgtgaa gccctgttta acgctattgc
    56341 cgcctacgcc agtaatattg aaaacctgcc tgcgctgctg ccagcggtag aaaaaatcgc
    56401 gcagaagcac accagcttcc agatcaaacc ggaacagtac aacatcgtcg gtgaacacct
    56461 gttggcaacg ctggacgaaa tgttcagccc ggggcaggaa gtgctggacg cgtggggtaa
    56521 agcctatggt gtactggcta atgtatttat caatcgcgag gcggaaatct ataacgaaaa
    56581 cgccagcaaa gccggtggtt gggaaggtac tcgcgatttc cgcattgtgg ctaaaacacc
    56641 gcgcagcgcg cttatcacca gcttcgaact ggagccggtc gacggtggcg cagtggcaga
    56701 ataccgtccg gggcaatatc tcggcgtctg gctgaagccg gaaggtttcc cgcatcagga
    56761 aattcgtcag tactctttga ctcgcaaacc ggatggcaaa ggctatcgta ttgcggtgaa
    56821 acgcgaagag ggtgggcagg tatccaactg gttgcacaat cacgccaatg ttggcgatgt
    56881 cgtgaaactg gtcgctccgg caggtgattt ctttatggct gtcgcagatg acacaccagt
    56941 gacgttaatc tctgccggtg ttggtcaaac gccaatgctg gcaatgctcg acacgctggc
    57001 aaaagcaggc cacacagcac aagtgaactg gttccatgcg gcagaaaatg gcgatgttca
    57061 cgcctttgct gatgaagtta aggaactggg gcagtcactg ccgcgcttta ccgcgcacac
    57121 ctggtatcgt cagccgagcg aagccgatcg cgctaaaggt cagtttgata gcgaaggtct
    57181 gatggatttg agcaaactgg aaggtgcgtt cagcgatccg acaatgcagt tctatctctg
    57241 cggcccggtt ggcttcatgc agtttaccgc gaaacagtta gtggatctgg gcgtgaagca
    57301 ggaaaacatt cattacgaat gctttggccc gcataaggtg ctgtaatttg atgttgccgg
    57361 atggaaacat ccggcaaccc ttgacgcggt ttaaattgcc gcgtcgtcct cttcaccggt
    57421 acggatgcga atgacccgtg ccacgtcgaa gacgaagatt ttaccgtcac cgattttgcc
    57481 ggtttgcgcc gtgcgaataa tggtatcaac acaggtatcg acaatgtcgt ccggtacgac
    57541 aatctcaatt ttcactttcg gcagaaaatc caccatatac tccgcgccgc ggtacagctc
    57601 ggtatggcct ttctggcggc caaaaccttt cacctcagtg accgtcatgc cggtaatgcc
    57661 gacttcagcc agtgcttcgc gaacatcgtc cagcttgaag ggtttaataa tcgcatcaat
    57721 ctttttcatg ctattccttg aaaaggtcgc ctgtcttttg atctgctaaa cgtaacacat
    57781 aacgccaatt cattccttga aatcgtttgc atccagctcg tgtcgggaaa gcagtttata
    57841 aaattctgtc cggttgcgcc ccgccattct cgccgcgtgt gtgacgttgc ctttggtgat
    57901 ttgcagcagc ttacgcaaat agttgagttc aaactgatta cgtgcctcaa caaaggttgg
    57961 cagcgccgta ttttcaccct ccagcgcctg ctctactaat gcatcgctaa taaccggaga
    58021 tgaggtcagc gccacgcact gttcaatcac gttgaccaac tggcgcacat tacccggcca
    58081 gctagcggtc atcaggcgtt tcatcgcatc agtggagaac gcgcggacaa acggtttatg
    58141 tcgctctgcc gcctggcgca acaggtgatt tgccagaagc ggaatgtctt ctgtgcgctc
    58201 tgccagagcc ggaattttca ggctgacaac gttgaggcgg taatagaggt cttcacggaa
    58261 ttccccgcgc gccatcgctt ttggcaggtc gcggtgagtg gcggaaataa tccgcacgtc
    58321 gatatcaata tcgcggttac tgcccagcgg gcgcacttta cgctcctgca acacgcgcag
    58381 cagtttgacc tgcaacggtg cgggcatatc gccaatctca tcgagaaata gcgtaccgcc
    58441 ttccgccgcc tggaataaac cttcgcgatt gctgacagcg ccagtaaacg cgccacgcgc
    58501 atgaccaaac agctccgact ccagcaattg ctcgggcaat gcgccacagt taatagcaat
    58561 aaatggtttg ctattgcgcg ggctggcgtt gtggatagcc tgggcgaaaa tctctttccc
    58621 ggtgccgctc tggccgttaa tcaaaacgct aacgtctgat tgcgccacca gccgcgcctg
    58681 ttccagcaaa cgcagcatca gcgggctgcg ggtgacaatc gcctcgcgcc agcgttcatc
    58741 ggtggctggc gcggattgtt ccagcgcatc gtcaattgcc tgatatagcg catctttatc
    58801 gacaggcttg gtgaggaaac tgaacacgcc ctgctgtgtt gcagcaacgg catcgggaat
    58861 agaaccatgc gcggtaagaa taattactgg cattcccggc tgcactttct gaatttcagc
    58921 aaacagctgc ataccgtcca tttcatccat ccgcaggtcg ctgatgacta aatctacttt
    58981 ttcgcgattc agtacccgta atccttcagc gccactttcc gccgtgacca cgctgtagcc
    59041 ttcgctggtc aggcgcaggc caagcagttt cagcaatccc ggatcgtcat cgaccaataa
    59101 taaatgcgca ggtttatggc tcatcaggag tgacctcatg ggtggatggc gcgggctttt
    59161 caccctcgtg cggcgtatcc ggcgagaaat ttccagccgg tttgcgggtc gagagctggc
    59221 gttcaatatc agtcaggttt tccagcttgc gggtggtgag ttccagttgc tgctgtagaa
    59281 cgtgatgttg ctggcgcaat gtatccagct cgctgtcgct ggcctgctgg agcttgccgt
    59341 agcgttggcg ctcttccgcc agttgcagtt gcagcgcctg accgtcgcgc cagagttgat
    59401 acagagggcg aacctgtgcc gggatctcgg tacttagcgc atcaatgcgc gcgaccagct
    59461 ggcggcgctc atacggcgta attttggcgt cggcgagcaa aatcccttgt ttaaaggtat
    59521 tttgccagct gccgtcgtca tattggcgag cttgctgacg tgactgcgca ggcattaaac
    59581 gatcagcaca atccatcgcc cgcagccagt aaagcggatt ggtttcggtt gatttacctt
    59641 gcagcgccca gatgtcgcta cattcagtag aaagatagtc agccagttga taaaccggaa
    59701 ttttttcttc tgctggcgta tcaatggctg gcttattgtg actctgcacg caacccagca
    59761 atgccagaca tggcagccct gccagccaca gccgtcgggg caataatcgt tgaaaaatgt
    59821 gtcgcatatt caccagactt aatgcctatc ccagtgggcg taattattgc aggcagtttg
    59881 gacatggaca gcgcggagaa accggagcgt acacgtcgta cgtgaggatt tcgagcactg
    59941 cccggggccg aactggcaaa taaaatagcc tgatgggata ggctgttagc attatttcgt
    60001 gtttttcgac gacggcaatt caatgcggaa acaaacgtct tgcccgctct cgtcgaccag
    60061 atacagttcc ccttgcatac ggcaaataca atccctggca atgcttaatc ccagaccgct
    60121 gcccttcacc gccccttttc gctggtggct tccttgaaaa aagggttcga agatcatggc
    60181 gcgttcctct tgcggaatgg gtgtgcctgt attgatgaca tcaatataaa cccgtgcacc
    60241 atgtaagctg ctgcgaaggc aaatgttacc ggattcagcc ccgtagtgca ccgcattgga
    60301 gtaaagatta tccagtacgc tcatcagcag cattggctcc gccaggcaag ctgttgcttt
    60361 gagatcgacg tcggtatgca tcattttagc ccgtgcgggc aggctatgag cagaaaccac
    60421 tgtctccacc agcggtgcta attcaacatt ctccagttcc accgcactgt ccgcctgttt
    60481 acggttgtaa tcaagcagtt gttcgatcag tttttgcaaa ttgcggctgc tgctatcaag
    60541 aatgctcacc acctcttttt gctctggcgt aagcggcccg acaacctggt cagccagtaa
    60601 ttcagtgccc tcgcgcatac tcgccagtgg cgtttttaat tcatgagata aatgtcttaa
    60661 aaattgatgg cgttgggatt ccagccatga caggcgctca cttaaccaaa gaatacgttg
    60721 cccaaccgag cgtaactcgc tcggtccact gaacgaaacg ctattgccca gagaacgccc
    60781 ttcccccaga cggttgatca tgcgctcgat atttttcacc ggcccgataa tcatccgcgt
    60841 gaaaagtagc accatcacca gactcaccag aaatagcacc agcgattgcc aaccaaaata
    60901 ttgcccacgt tcggcgattt cacgctgaag ttgctgccca cgagagaaca ccactgtgcg
    60961 cgtggcctgc accatttcgg tattggcact ggcaaaggct tcaagacgcg cggcggcggc
    61021 agcatcggga ccactgttgt tacactgaag ttgggccaga ttgttcaaat cctgacgcaa
    61081 tgcctggtaa agtttatcgt ctggcagtac gcctgcgtgg gcatcgagca tttcgctgta
    61141 acgcttgcgc tggctttgat aaaccttcgc aagcgtaggg tcgtccagca cgcaatactg
    61201 acggtagcta cgctccatct ccagcgccgc gttggtcatc gcttcactac gccgggcatc
    61261 aataagcgta gtgcggttaa ccagcgccgc ctgatcgcta agcgcattca ggctttgcca
    61321 ggcttgccat gccagcacca acaggggtag tagaattagc aaaaatgcca gcattaccag
    61381 ttgtcgtaat gagcggggaa aaacgggcca gcgtttcaag gtgttactct cgtcagacgc
    61441 gaatagcctg atgctaaccg aggggaagtt cagatacaac aaagccggga attacccggc
    61501 tttgttatgg aataaggcgg tgcctaactc gacgtttcgc ccgatggttg atatagctac
    61561 gctgatatca gaagttggac ggcaggcacc ttgttgtgcg tcattcgaat tttatgtagc
    61621 acatcccgaa ggggctgaca taagtcggtg aatgagccac tgcttactat tatgcagtaa
    61681 ctgtgccaat aaagaaaata gtttggtaac gtactgatta tacgttgctt tgagggattt
    61741 atggcttctt cgctatttga atgtaaatca gccactgtgt cgctaaaaag agacaactta
    61801 agataaactt attagattac aataaaatca atcatttaag tgtcgccaga aagcgacacg
    61861 gtaaaccacc cattgtcgtg atttacagac acaaaaaagc tcccggagtt gggagcttat
    61921 gatagtggtt ggtgcttaat gccggatgcg gcgtaaatgc cgcatccggg ctgcaaaacc
    61981 aatgaactga tgacttaccc caactgctta cgcgcattgc ggaaaatgcg catccatggg
    62041 ccatcctcgc cccagttttc cggatgccag gagttgctga cagtacggaa aacacgttcc
    62101 gggtgcggca tcataatggt gactcgacca ctttcagtcg taaccgacgt aataccgttc
    62161 ggagaaccgt tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt atcgacatag
    62221 cgcagtgcca ccagcccttt gctttccagt gccgccagat gcgccgcatc acgcacttcc
    62281 acgcgccctt caccgtgaga gacagcaatc ggcatttgcg agcccaccat cccctgcaac
    62341 agcagcgacg ggctttgggt cacttcaacc aggctgaaac gcgcttcaaa gcgatcggag
    62401 gtattgcgca caaaacgtgg ccacaactca ctacctggga tcagttcacg cagattagac
    62461 atcatctggc aaccgttaca tacccccagc gccagcgttt gcggacggtg gaagaaggtt
    62521 gcaaactcat cgcgtacacg gtcattgaac aggattgact tcgcccaacc ttcaccggca
    62581 cccagcacat caccgtagga gaaaccaccg cacgcgacca gggcgtggaa atcttccagg
    62641 cccgtgcgtc cagccagcag gtcactcata tgcacgtcga tagcatcaaa gcctgcacgg
    62701 tggaaagctg ccgccatttc aacatgcgag ttcacgccct gctcacgcag tacggcaact
    62761 ttcgggcgcg cgccagtggc aataaacggt gctgccacat cttcgttgat atcgaacgac
    62821 agttttacat tcaggcccgg atcggcgtcg tttgatttcg cctggtgctc ctggtcagca
    62881 cactccgggt tgtcacgcag gcgctgcatc tgccaggtag tttctgccca ccagacacgc
    62941 aacgtagtgc ggctttcgct gaatacagtc tgcccgttgg cggtaatcac aaaacggtca
    63001 ccggaaaccg cctgcccaac ataatggacg caatcagcaa gcccatgctg tgccagtacg
    63061 gactcgaccg cttcacggtc agcggcacga acctgaatca ccgcacccag ttcttcgtta
    63121 aacaacgccg ccaggcgatc gtcacccaga gagccgatat ccgcatcaat gccacaatga
    63181 ccagcaaagg ccatttccgc cagtgtgacc aacagaccgc catcagagcg gtcgtgatac
    63241 gccagcagct tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc tttcagttgc
    63301 gcgacatcgc gtacatctgc cggtttgtcg ccaagctgac gataaacctg cgccagcgcc
    63361 gttgcgccca gcgcgttatt gcctttgccc aaatcaatca gcagcagtgc gttatcttcg
    63421 gtagaaagct gcggcgtgat ggtgtgacgt acatcttcca cgcgggcaaa tgcagaaatc
    63481 accagcgaca gcggcgacgt catttcgcgc tcttcgttac cttcctgcca gcgggttttc
    63541 atcgacatag agtctttacc caccgggatc gtcaggccca gcgccggaca aagctcttcg
    63601 cccacggctt taacggcttc atacaggccc gcatcttcac cagggtggcc tgccgccgcc
    63661 atccagttgg cggaaagttt aatgcgtttg atatcgccaa tctgtgttgc ggcaatattg
    63721 gttaacgctt caccgaccgc cagacgggca gaggcggcga aatccagcag cgcaaccggc
    63781 gcacgctcgc caatcgccat cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc
    63841 gcgcagttag cgaccggcac ctgccacgga cccaccatct gatcgcgcgc taccatgcca
    63901 gttacgctgc ggtcaccgat ggtcaccagg aaagttttct ccgccacggt cggcagatgc
    63961 agtacgcgtt tcaccgcgtc tgcaatggtg atcccttcac ggaccagcgc atcgcccttc
    64021 gctttcagcg tttgtacatc gcgggtcatc ttcggcgttt taccaagcag gacgtccagc
    64081 ggcagatcga tcggctgatt atcaaaatga cgatcgtgca gagaaagatg cagttcttcg
    64141 gtcgcttcac caatcaccgc gtagggtgcg cgctcacgct tacacagttc gtcaaacagc
    64201 ggtaattgat cggcagcaac cgccagcacg tagcgttcct gggattcgtt acaccagatt
    64261 tccagcgggc tcatgcccgg ttcgtcgctt agaatatcgc gcagttcaaa tttaccgccg
    64321 cgtccgccgt cgctcaccag ctccggcatc gcgttagaaa gaccgcccgc accgacgtcg
    64381 tggataaaca gaattgggtt agcatcacca agctgccagc aacggtcgat cacttcctgg
    64441 caacgacgct ccatctccgg gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca
    64501 gactgacctg acgccatgga agacgccgct ccgccgccaa gaccgatgtt cattgccggg
    64561 ccgccgagaa cgaccagctt cgcaccgacg ttgatctcgc ctttttgtac gtgatcggcg
    64621 cgaatgttgc cgatcccgcc cgccagcatg atcggtttgt gataaccgcg cagctcttcg
    64681 ccgttgtggc tgttcacttt ttcttcataa gtacggaagt agccattcag tgccggacga
    64741 ccaaattcgt tgttaaacgc cgcgccgccc agcgggcctt cggtcatgat gtccagcgcg
    64801 gtgacaatgc gctcaggctt accgaaatct tcttcccacg gctgttcgaa gccaggaatt
    64861 cgcaggttgg aaacggagaa accaaccaaa cccgctttcg gcttcgcgcc gaggccggtg
    64921 gcaccttcat cgcggatttc accgccggaa ccggtcgccg cgcccggcca cggagaaatc
    64981 gccgtcgggt ggttgtgagt ttcgactttc atcagaatat gcgccggttc ctgatggaaa
    65041 tcgtagcggc ccgtttcgtg gtcagcaaag tagcggccca cttcagaacc ttccattacg
    65101 gcggcgttat ctttataagc agagagaacg taatctggcg tggtttcgaa ggtgtttttg
    65161 atcatcttaa acagcgattt cggctgttgc tcaccgtcga taacccaatc ggcgttaaaa
    65221 atcttgtggc ggcagtgctc ggagttcgcc tgggcgaaca tatacagctc gatgtcgttc
    65281 gggttacgac caagctttgt gaaagcatcc tgcagatagt caatttcatc ttccgccagt
    65341 gccaggccaa gacgcaggtt agcgtcgatt agcgcctgac ggccctgccc cagcaaatca
    65401 acgctggtga ccggagtcgg ttgatggtgt gcaaacagct gctctgcatc atctaaagca
    65461 aaaaagaccg tttccatcat gcggtcgtgc agttcagcgg taacctgctg ccattgttca
    65521 ttggtcagcg taccggcttc tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt
    65581 tgtagcccgc agttatgggc aatatcggtc gctttcgaag accagggaga gatggtgcca
    65641 ggacgcgggg tcaccagcag gagtttgcct tgcggggcgt ggctggcgag tgccgggcca
    65701 tatttcagca ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca
    65761 gcaaaatgga catactcggc gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt
    65821 gccagcagtt tgttgattcg gaatgccgac agtgcaggcg aaccacgcag aatttccatc
    65881 ataagtctct cgtcttctaa gctttcggtg tacccaaggg ggggaaacgg gcgtcattat
    65941 aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta
    66001 gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg
    66061 ccgctcattt aagagtaaac ctttcactat tattacgttt tttcaagctg ggacgcgcac
    66121 gacacagaga attaactaat tgaaaaaatt aaagattaat tatctgttca tcggcattct
    66181 ggcactgctg ctcgcggtcg ctctctggcc atccattccc tggtttggta aagccgacaa
    66241 ccgtatcgcc gccattcaag cgcggggaga gttgcgtgtg agcaccattc atactcccct
    66301 gacttataac gaaatcaacg ggaaaccttt tggcctggat tacgaactgg cgaaacagtt
    66361 tgccgattac ctcggcgtaa aactgaaagt gaccgtgcgg cagaatatca gccagctgtt
    66421 tgacgacctt gataatggta acgccgacct gctggcggca ggactggtct ataacagtga
    66481 gcgggtaaaa aattatcagc ctggccctac ctattattcc gtgtcacaac aactggttta
    66541 taaagtgggt cagtatcgcc cacgtacgct gggcaacctg acggcggagc aactcaccgt
    66601 tgcaccgggt catgtggtgg ttaacgatct ccagaccctg aaagaaacaa aattcccgga
    66661 attaagctgg aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga
    66721 aggaaaactc gattacacca ttgctgattc tgtcgccatc agcctgtttc agcgcgttca
    66781 cccggagctc gccgtagcgc tcgatatcac cgatgaacaa ccggtgactt ggtttagccc
    66841 gttagatggc gataataccc tttccgccgc cctgctcgac ttcttcaacg aaatgaatga
    66901 agacggtacg ctggcacgca ttgaagagaa atacctgggg catggcgatg attttgatta
    66961 cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt taaagcccct
    67021 gtttgagaaa tacgccgaag aaattgactg gcgtttgctg gccgctattg cttatcagga
    67081 atcgcactgg gatgcacagg ccacttcacc gacgggtgtg cgcggcatga tgatgttaac
    67141 caaaaatacc gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac agagcatcag
    67201 cggtggcgtg cgttatttgc aggatatgat gagtaaagtg ccggaaagtg tgccggagaa
    67261 cgagcggatc tggtttgccc tcgctgcgta caatatgggc tatgcgcata tgctggatgc
    67321 ccgcgccctg acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg taaaacagcg
    67381 tctgccttta cttagccaga aaccctatta cagcaagctg acttacggct acgctcgtgg
    67441 acatgaagcc tacgcttatg tcgaaaatat tcgtaagtat cagattagcc tggtgggtta
    67501 tctgcaagag aaagagaagc aggctacaga agcggcgatg caactggcgc aggattatcc
    67561 ggcggtatcg cctacggagt tgggcaaaga gaaatttcct tttctctcgt ttctttccca
    67621 gtcgtcatca aactatttga cccattctcc ctctctgctg ttttccagga aagggagtga
    67681 agagaaacaa aattaatccg tcgaggattg cgcttttttc tgcgctttaa tttcctggcg
    67741 gcgcatgcga aagaagtcac tgagcaacgc cgcgcactca tccgccagta ttccttccgt
    67801 aatttccact cggtgattca tacccggatg atgcagcaca tccattaaag atcccgcagc
    67861 gccagttttc gcgtcacgcg caccaaagac cacgcgacca atgcgactgt ggatcatcgc
    67921 tccggcacac attacacatg gttcaagcgt gacatacaac gtggcgtcga tcagacgata
    67981 attttgcatc accagaccac cctgccgcag ggccatgatt tctgcatgtg cggtgggatc
    68041 atggcgacca atcgggcggt tccagccttc gccgattacc cgattgttat gcactaatac
    68101 cgcgccgacc ggcacttccc gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg
    68161 catccagtat tcgtggctaa attcgacttc agacaaaaag aaaactccgg ttataaaagc
    68221 gcggcgcatt atacacggac gctatgcttt actctattcc agttgctgga gttcaccgcg
    68281 cggggttact cgccaacgat gctgacagaa ataaagcagc ggattgtcct gattactgtc
    68341 gctatagcca ctgtacagcc gcagcggagt gccgattttg cgctccagtt gtgcgacctt
    68401 ttcatgtccc agacaacgca tcgtcaatac ccaaccacca tagccacgct gaatttggct
    68461 ggcgataaga ttaacccgcg gcagccaggg cgtatcgaaa taaaccgctt caaccagcgg
    68521 ctgcggagag ccggtaatca accagatatc agcatcggaa cttaacaggt aggtggttaa
    68581 tcgctcctga accagcggaa aggcggtaac attgtcgcga aaccagcgca cgaaatcggc
    68641 ctgcaacgtc tgtaaacgtg cttcgctgtg accaaaagtg cacccccaca gaagcagact
    68701 catcggccag cgtgccgcac gaccttttat caataacgca atggctataa tcggtaacaa
    68761 cggcaggaca agtaacgcat tcagcggttg gcgacgcagt aaatagcgca gaaaactgcc
    68821 gaacatatcc tgctgatgca atgttccatc taagtcaaaa aacaccacac gacgctcgtg
    68881 agttgccaaa ccatactcct ctggactgaa acatcctgat taattttcgc tactacatag
    68941 cctaacagat agatcatcac ttttccggca acaatcgacc gcatcatggc taactggaat
    69001 ttttaattca tggcaattag cggcaatgga atataaaatt cactcgcgtg tctctcatat
    69061 tgcctgttgt cgccactatt tcatcgcgag gaaagatatg aacggcttac ttcgtatccg
    69121 tcagcgttac caggggcttg cccagagtga taaaaaactg gcggattatc tgctgctaca
    69181 acctgatacg gcgcgccatt taagctctca gcaactggcc aacgaagccg gagtcagtca
    69241 gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa ggttttccgg cgcttaagtt
    69301 ggcgttgagt gaagcgctgg caagccagcc ggaatcgccc tccgtgccca ttcataacca
    69361 aatccgcggt gatgatccgt tacggctggt cggcgaaaaa ctgattaaag aaaatactgc
    69421 cgctatgtac gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat
    69481 gttgcgctct gcgcggcgga taattctgac cggtattggc gcttcgggtc tggtggcgca
    69541 aaactttgcc tggaagctga tgaagattgg cttcaatgct gccgcagtgc gcgatatgca
    69601 tgcgctactc gcaacagtac aggcgtcgtc ccctgacgat ctgttattag ccatttccta
    69661 caccggtgta cgacgcgagt taaacctggc ggcagatgag atgctgcgag tgggcggaaa
    69721 agtgctggcg attaccggct ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct
    69781 gtataccatt gccgaagaac aagcgacaaa cagcgcttca atctctgctt gtcacgctca
    69841 gggaatgtta acggatttgc tgttcattgc gctgattcag caggatctgg aactggcacc
    69901 agaacgtatt cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg
    69961 tataatgccc gcccggtttg tgttgttttg agagtttcct tatggcgttg ttaatcacta
    70021 aaaaatgcat caattgtgat atgtgtgaac ccgaatgccc gaatgaggcg atttcaatgg
    70081 gagatcatat ctacgagatt aacagcgata agtgtaccga atgcgtaggg cactacgaga
    70141 caccaacctg ccagaaggtg tgcccgatcc ccaatactat tgtgaaagat ccggcgcatg
    70201 tcgagacaga agaacagttg tgggataaat ttgtgctgat gcaccacgcg gataaaattt
    70261 aatattctac tctggaagta gagtattaat tatattactg ggaagcctta acgccattat
    70321 atttatttaa ttgatgacat tagcataatc attcactaag ttaatttata tagtatctgc
    70381 caagacactt atttatagtt attaaaggcg cgtccgattg gttcaccgga cgcaccttaa
    70441 gtacgtttcc ttgtgttata agaacagaag gatcagctgt aaaacagcaa tgatgatttt
    70501 gatgacccgt ttaatcaggt atcggcaatc agtcattcgt ttttccttaa acaaggaatg
    70561 cagccgttca gattagccct tacatctccc caaaactgaa cgtgcgagtt attgagggtg
    70621 catgctgcac tccacaccag agctttgacg acaccactcg tttcaatggg ggaattctgt
    70681 ggcatggtgt aaagcacagc aaaatcttca ataacgaagc caattttaat gtacttacga
    70741 attggcggtc acgttaatct ttccatcaat attgctttct tcgtaaaggc tcgagttttt
    70801 atgctaaaga ttgcaagttg cttgtaaaag ataagtacac tgatccataa tcgctgttgt
    70861 tgagggtgca tgctgcacaa aattaaagtt aaaaagtaaa acccccgttc cttaccagtt
    70921 cgggggtttt actttttaaa gagaacggta ttattttaac tttcaataat taccgtggca
    70981 caagcatagt ggcgttcatc tgccagcgtt acatgcatat ttacaacgcc gagcttttcc
    71041 gccagtttta atgcctcacc ccatagccgt agccgtggtt tgccgagctc atcattgaat
    71101 acttcaaatt gattaaacgc cagaccattg cggatcccgg tgccaaacgc ttttgctgcg
    71161 gcttctttca cagcaaaacg cttcgccaga aaacgcaccg gctggtggtg cgttttccag
    71221 attgcccatt cgttatcgct taatacgcgg cgtgccaggc gctcaccgga tcgggcgatc
    71281 actgcttcta tgcgagcgat ctctacgata tccgtgccta aacctaatat tgccattagc
    71341 cacgcgcttc cagcatcaga cgcttcattt ctgccaccgc atctttcagt ccggtcatca
    71401 ctgcacgacc aataatggca tgaccgatat tcagttcatg catctcaggg atggcggcaa
    71461 tagctttcac gttgtgatag gtcagaccgt gaccagcgtt aactttcaga ccgagactag
    71521 cggcaaaggt cgcggctttg gcgatacgcg ccagctcttg cgcctgttcg gcgtcagttt
    71581 tggcatcagc atagcaaccg gtgtggatct cgataaacgg tgcgccaacc tctgccgcag
    71641 ctttgatctg ctcttcatcg gcgtcgataa agagagaaac ctggatcccg gcatctgcca
    71701 gacgtttgca ggcatcgcgc attttgtcac gctgccctgc gacgtccagg ccgccttcgg
    71761 ttgttacttc ctgacgcttt tccggtacca gacagcaaaa atgtggcttc gtctcaacgg
    71821 cgatcgccag catctcttcg gtcaccgcca tctccagatt catgcgggta tccagcgtct
    71881 gacgcaggat gcgcacatca cggtcagtaa tgtggcggcg atcttcacgt aaatgcacgg
    71941 taatgccgtc cgctcccgcc tgctcagcaa taaacgccgc ctgcaccgga tccgggtaag
    72001 cagtaccgcg tgcgttacgc agcgttgcaa tatggtcaat gttgacgcct aacagtaatt
    72061 cagccatgac aatcctcatc attcataatg tgttttcacc gttcgcttag gcataaactg
    72121 ccggaacagt tccctgctct ttaatggttt accgccaaga tacggcttaa gcgccatgcg
    72181 ggtaaagcgt ttcgcggcgc gtagtgtgtc tgcgtcagga aattcccgcg cgtttaacgc
    72241 ttttaactgc cttccggtga acgttttatt gtcgataacg acgcttgcga taaacccttt
    72301 ttcttcgcga taacgatacg tcatggtgtc atctaccggc tcgccgctac ccgcacaatg
    72361 ggtaaaattg acgccatagc ccagatgccc gagcagtgcc agttcaaagc ggcgcagcgc
    72421 gggttctggc gtaccagtgg cacctgcaag agactgaatg cagtgcaagt aatcgaaaaa
    72481 gagttcagag aagcgcgtct cgtattccag tacgcgggag agaagttcgt tgatgtacag
    72541 accgctgtaa agcgtgatac cgcttaatgg cagcgccagc gagacagctt cagcactgcg
    72601 cagcgttttg acttcgccac gcccgccgaa acgtagcaag agaggggtga aaggctgtaa
    72661 tgcacctttc agagtagagc gtttagagcg tgcgcctttg gcaaccagac gcacgcgacc
    72721 cgattcctcc gtgaagacgt ccagcatcag gctggtttcg ctccacgggc gactatgcag
    72781 gacaaatgcg cgctgccagc cttccatcgg agtgactctt aaagatcgtc aacgtaaccg
    72841 agactgcgca gtgcgcgttc gtcgtcggcc caaccggatt tcacttttac ccacagttca
    72901 aggtggacag gcgcttcgaa catttcctgc atgtctttac gcgcttcaat cccgatggtt
    72961 ttgatcttgg cccctttgtt gccaataacc atcttcttct gcccttcacg ctcaacgaga
    73021 atcaaaccat tgatgtcata accaccgcgt tcgttagaga cgaaacgttc gatctccacg
    73081 gtcacggagt acggcagttc agcgccgagg aaacgcatca gtttttcgcg gatgatttca
    73141 gacgccataa aacgctgtga gcgatcggtg atgtaatctt ccgggaagtg atgggtcgct
    73201 tcagggagat gcttacgcac gattgctgcg atagtgtcga cattcagccc ggtttcagca
    73261 gagatcggca cgatatcgag gaagttcatc tgacttgcca ggaactgtaa gtgcggcagc
    73321 agatcggctt tttcctgcac gttatccact ttgttcaccg cgaggatcac cggcgctttg
    73381 ccatcgcgca gtttgttgag caccatttcg tcgtccggcg tccagcgggt gccttcaacg
    73441 acaaaaatca ccagctcaac gtcgccaata gagctgctcg ccgctttgtt catcaggcgg
    73501 ttaatagcgc gtttttcttc catatgcagg ccaggggtat caacgtagat cgcctgatac
    73561 gcgccttcag tatggatccc cacaatgcgg tgacgagttg tttgcgcttt gcgggaggtg
    73621 atggagatct tctgccccag cagtttgttc aacaatgtgg atttgccaac gttcggacgt
    73681 ccgacgatgg caataaatcc gcagtaactt ttatcgatgc tcattccagc tccagttttt
    73741 tcaacgcctg ttcggcggca gcctgctcag ccttacgacg gcttgaacct gtgccaacca
    73801 ccggttcact caggccgctg acctggcagt ggatagtaaa ttcctgatcg tgcgcttcgc
    73861 cacgtacctg gactaccaga taagtcggca gcggcagatg gcgaccctgc aaatattctt
    73921 gcaagcgcgt tttcggatct ttttgtttat cgcctgggct aatttcgtcc aaacgagttt
    73981 gataccagtt gaggattaat ttctcgacgg tttgaatatc actgtcgagg aatacaccac
    74041 caattaatgc ttcgacggtg tcggcgagaa ttgactcacg acgaaatcca ccgcttttaa
    74101 gttcacctgg ccctaaacgt aagcactcgc ctaactcaaa ttcgcgcgcc agttccgcca
    74161 gcgtattgcc acggaccagc gtggcgcgca tccggctcat atcgccttca tccacacgag
    74221 ggaaacggtg ataaagcgca ttggcgataa cgtagctcag aatagagtcg cctaaaaatt
    74281 ctaaacgctc gttatgtttg ctgctggcac tacgatgagt taatgcctgc tgcaacagtt
    74341 cctgatgatt aaaagtgtag cccagcttcc gttgaagccg attaattacg atggggttca
    74401 tgcgatacca ataaataaat gcgtcaacaa ttcagcacac gaaacagacc tgatatacat
    74461 ggctctgcta actgcttcgc tgcagtttca gtttaccggt atatggggac caacgctgtt
    74521 tcgtgtgccg tggcaacctg gaggtgccaa ccttaaactt cgggggaata ttctatacac
    74581 aacgacgggg gatgtcgtta gccacgggag atttatctca taaataattc acgttgtcgc
    74641 cataacggcg acaacgtgaa cgaagatggg ctattaatgg atgccgccaa tgcgacttaa
    74701 gcgcacacct gtcggccatt cgccttcttg cttatcgaaa ctcatccaga tagccgttgc
    74761 ccgaccgacc agattcgctt caggcacaaa accccagtaa cggctgtccg cgctgttgtc
    74821 gcggttgtcg cccatcatga agtattgtcc cggcggaaca atccaggttg ccagttgttg
    74881 ccctggctgc tggtaataca tccccacctg gtcctgcgca atcggcactg tcagaattcg
    74941 gtgcgtcaca tcacccagtg tctctttacg ctcggaaaga cgaattccat tttctttggt
    75001 ttcgtttttc ggcacttcaa agaatccgct ggtcgcttcc ccaccattac ggcgtgagaa
    75061 ggtctgaacg aaatcgctcg gttccacgtt ggagtaggtg accggtagcg cgttttcaca
    75121 cgcctggccg gaactgcatc ccggttgaat cgtcagctct tttgagaccg gatcgtaagt
    75181 gactttatcg cccggtaaac ccaccgcgcg cttgatgtaa tcaagctttg gatcttccgg
    75241 atatttaaag accacgatat cgccgcgttt cggatgaccg gtttcgatca gcgttttctg
    75301 gtagatagga tctttaatgc cataagcaaa cttctctacc agaataaaat caccgattaa
    75361 cagagtcggc atcatcgaac ctgacgggat ctggaacggt tcataaataa acgaacgtac
    75421 aatcaatacg atagccagca ccggaaaaac agaagcaccg gtttccagcc agccaggctt
    75481 cggcgcaacc tttttcaggg ttgctttatc cagtgagtca ccggcagccg cctgcgccgc
    75541 tgcctgacgt tcccgccgtt taggtgcgaa aaagaattta tccacgcacc ataaaatgcc
    75601 cgtcaccagt gtggcaatca ccagaatcag ggcaaacata ttcgccatgc caactcctaa
    75661 gggttatttg ttgtctttgc caacgtgcag aatggcgagg aacgcttcct gcggcagctc
    75721 gacgttaccg atctgcttca tgcgtttctt accttctttc tgcttctgca gcagcttttt
    75781 cttacggctg atatcgccgc cataacattt agccagtacg tttttacgta gctgtttcac
    75841 ggtggatcgc gcaataatgt gcgtaccaat cgctgcctga atggcgatat caaactgctg
    75901 gcgtgggatc agatctttca tcttctccac caactcgcga ccgcggtttt gcgaattatc
    75961 acggtgggtg atcagcgcca acgcatcaac acgttcaccg ttgattaata cgtctacacg
    76021 taccatgtcg gacgcctgga agcgtttgaa gttgtaatcc agagacgcat aaccacgcga
    76081 ggtagatttc aggcgatcga agaagtcgag caccacttcc gccatcggga tctcgtacgt
    76141 cagcgccacc tgattaccgt ggtaaaccat attggtctgc acgccgcgtt tttctacgca
    76201 cagcgtaata acgttgccga gatatgcctg cggcagcagc atgtgacatt cggcaatcgg
    76261 ctcgcgcagt tcgtagatgt tatttaccgc tggcagcttg gatggactgt cgacgtaaat
    76321 aacttctctt gacgtggttt caacttcata cactacggtc ggcgcagtgg tgatcagatc
    76381 cagatcgtat tcacgttcca gacgttcctg gatgatctcc atgtgcagca ggccgaggaa
    76441 gccgcagcgg aaaccaaagc ccagcgcgct ggagctttcc ggctcataga acagtgaagc
    76501 atcgttcagg ctgagtttac ccagcgcgtc acggaaggct tcatagtcgt cggaacttac
    76561 cgggaacaga ccggcgtata cctgcggttt gactttctta aagccaggca gcgccttttc
    76621 tgccggatta cgcgccagcg ttaaggtatc gccgactgga gcgccgtgga tatctttaat
    76681 cgcacatacc agccagccta cttcgccaca tttcagttca gtgcggtcaa cctgtttcgg
    76741 cgtgaagatg cccagacggt cggcgttata ggtctgcccg gtactcatga ctttcacttt
    76801 gtcgccctta cgcagggtgc cgtttttaat acggataagt gaaacaacgc ccaggtagtt
    76861 gtcgaaccat gagtcgataa ttagtgcctg caacgggcct tccggatcgc cttccggcgg
    76921 cggaatgtcg cgcaccagac gttcgagaac gtcctgcacg ccaacgccgg ttttcgctga
    76981 acagcgcacc gcgtcggtgg cgtcgatgcc gacgatatct tcaatttctt ccgccacgcg
    77041 ttcaggatcg gctgccggca ggtcaatctt gttcagtacc ggcaccactt cgagatccat
    77101 ttccatggcg gtgtagcagt ttgccagggt ttgcgcttct acgccctgcc cggcgtcgac
    77161 caccagcaat gcaccttcac aggcagccag cgaacgggaa acttcatagg agaagtcaac
    77221 gtggcccggg gtgtcgataa agttcagctg ataggtttcg ccgtcagacg ctttgtagtc
    77281 cagcgtcacg ctttgcgctt tgatggttat gccacgctca cgctcaagat ccatggaatc
    77341 gagaacctgc gcctccattt cacggtcaga caggccaccg cagatctgga taatacggtc
    77401 agacagcgtc gatttaccgt ggtcaatgtg agctatgatc gaaaagttac gtatattctt
    77461 cataaagtat gattattgtg ccttaatgcc cggttaacca ggcttttaga agtcgctgtt
    77521 ctgagcttaa cgtctgtatt aatagaaacg ccgcattcta cactacaaca ttgaggcgag
    77581 gaaatgttca taccgtatgg attgtggtat ctggaaacgt cctcgcattt gttatgcaaa
    77641 atgcaacaaa gccagtgaaa tcactggctc gcgtcttccg aagatgtttc aaatcgcaca
    77701 aggccaggcg gcaatgccac gcttaagatg atcggttgcc attctgcccg ggcagcaaac
    77761 ttacgcgagt agccgcgcgc aatcaggaac ccgccaatac cgccgaggac cgcaccacat
    77821 aatgctgcga cgtcagaagc aaagagtagc tgaaatagcg aagcgatgag gaataatccc
    77881 accagcggcg acatataaac cagtaatgcg gagctaagca ggctgccttc ggcgatcccc
    77941 aattccactt tttgccccgg cactaacggc tcatcacagg gtacgacaat ggtatgcgtg
    78001 gtttgcgggc caagtttatt taacacgcgg ctaccgcaac cggcgcgtga agcgcagctg
    78061 ctgcatgaaa ctttaacatc acaactgacc agcgcctgcc cgttttgcca ggagacgacg
    78121 gtagcccact ctttgatcat tgcgctgccc cgaacttaat attctcggca atgcgtttcg
    78181 ccgtttgcgg cggcagttca ccgacaatgg tgatttcggc gttatcacgt acgcttgtac
    78241 tgacggttct gcgtccggtg cgcaacatct gatcggtgct cgatggcgta gcgcggttaa
    78301 cgtttaccga gaagctgaat aatccgtcgg aatagagacg tgattcgata ggcatgttgt
    78361 ccatcgtcgg tagcggacgt cgactactgg aaacttcgct aaaaccctgt ggcaaccagg
    78421 ttggcgtcca gctgaattta gctttttcac ctacaggaac agaaagcaac ggcggcaaat
    78481 ttgccttcgc cagcgtctgc atactgctac tgatatcctg attgacgtta aaagcaatca
    78541 cgcgaaattg ttccagcgtt tcaccatcgc gatcaaggag atcaaccctc atcggtaatt
    78601 tcgattcgat gtccatccac acgatgtagc tgtagcgtgt accatctcgg gcaaccacgc
    78661 gaatgacttc gcaaagacga tcagcaatac gcgtgcgccc gacggagata aagtcgtagt
    78721 aaggagaaag gcgtttgaaa tcggtataga tgagcgatgg cagagaatca acaatgtaat
    78781 cgccattaag cgtgaacggt tcaagtcccg gttcaaaata gctgatttca ttgccacgct
    78841 gtaccacttc ccggcgcggg ccatccattt gcaacaattg tgcaagagga cggttatcga
    78901 ggcgtgcatg tcgataacgc agagactcaa caccctgttt attgatgctg atgaatgaca
    78961 gctcgtaatt cagtgactga ctggccaggt tcatctgctg taataacgcc ccggacgcgg
    79021 gagtggccga ggcgttagca gagaataaca ggctacctgt cactaatgac atggcaaacc
    79081 aaagttgctt cattactgcg attgcgttcc taaagtttga attcctggca cctgtacagc
    79141 ggcttgctgg gtttgcgcct gctcaaactg aagctgttca gagtggagtc ggcgttgcag
    79201 ttcgtaatcc tgcaacattg cattaatgcg acgacgctgc tcctgtacct gctgctgttg
    79261 accattgttt gcggtcgctt cagaaggtac tcccaggctt accgggctgg ctttacccat
    79321 catcggcagt gtattaaata ccggcgtttc gggctgctgg gacgtttcag attgtccatt
    79381 atagtgctgg acgccaacga taactgcaag cgatacgcat gctgctacgc ccatttgggt
    79441 aagctgtgcc gcccacggac gtactttctg ccagaatggc attttctgcc attgatgcgg
    79501 cgcaggctgg gcttccggga tcaatgtcgc cggttgacgt actggctctt cttcaatggc
    79561 ggccatcacg cgtgaagaga tatcgaaatg gagcacctcg ggagtatcac cccgcattga
    79621 gtcacggatt aagtgatagc tttcccaggt tttctgcatt tctgggttat gagccagttc
    79681 gttaagcagc tcactatcca gcgtttcgcc atccattaaa gcggaaagtt gttctttctg
    79741 catgcctaat acccttatcc agtatcccgc tatcgtcaac gcctgataag cggttgaact
    79801 ttgttatcaa tagcttccct cgctcggaag atacgtgaac gcaccgtacc taccggacaa
    79861 tccatgatag cggctatctc ttcatagctc aggccatcca gctcccgcaa ggttattgcc
    79921 atgcgcaaat cttccgggag ggactcaata gttcggaaaa ctatctgtct cagttcttct
    79981 gacaacatta agttctcagg gttcgaaatt tctttcaacg cgccgccact ttcgaagttt
    80041 tcagcttcaa tggcatccac atcactggaa ggtggacgac gcccctgagc aaccaggtaa
    80101 tttttcgctg tatttacagc aatccgatac agccatgtat aaaaagcgct atctccccgg
    80161 aacgaatcca gcgcacgata ggctttaata aaagcttctt gtaccacatc gggaacatca
    80221 cccgacggca catagcggga aaccagactc gccactttat gctgatagcg cactaccagt
    80281 aggttaaagg ctttctgatc tcccttctgg acccgttcaa ccaggacctg gtccgttaac
    80341 tgctcgctca tccgaggtaa agtctcccca aaccaaattt ccacgcgcta tcgaaacgcc
    80401 actccattag ctgcaatttg agcaagcaaa gggttagagt gtctcgtttt tgtaaagttc
    80461 cgtaacgcat ctgtttttgt ttgtcatgct gtagacggat cattatctat cattataagt
    80521 ctacagaatc tgaacatcgc attatctgtg tagaaatgcc catttaactg cctgaagagt
    80581 aacccaacgg cctttttatt tcaccaccta atcctccacc agccagtaac ttctcttttt
    80641 ctcgccgccc tgcgtcagcg tgtttagcca ctgtaacaaa tattaaaata gcaggtgttt
    80701 attcgcacaa catgatgcta tgctgaccaa acggtgttta gtaaattaaa caaagaaaat
    80761 gaatactctc ccagaacatt catgtgacgt gttgattatc ggtagcggcg cagccggact
    80821 ttcactggcg ctacgcctgg ctgaccagca tcaggtcatc gttctaagta aaggcccggt
    80881 aacggaaggt tcaacatttt atgcccaggg cggtattgcc gccgtgtttg atgaaactga
    80941 cagcattgac tcgcatgtgg aagacacatt gattgccggg gctggtattt gcgatcgcca
    81001 tgcagttgaa tttgtcgcca gcaatgcacg atcctgtgtg caatggttaa ttgaccaagg
    81061 ggttttgttt gatacccacg ttcaaccgaa tggcgaagaa agctaccatc tgacccgtga
    81121 aggtggacat agtcaccgtc gtattcttca tgccgccgac gccaccggta gagaagtaca
    81181 atccacgctg gtgagcaagg cgcagaacca tccgaatatt cgtgtgctgg agcgcagcaa
    81241 cgcagttgat ctgattgttt ctgacaaaat tggcctcccg ggcacgcgac gggttgttgg
    81301 cgcgtgggta tggaaccgaa ataaagaaac ggtggaaacc tgccacgcaa aagcagtggt
    81361 actggcaacc ggcggtgcgt cgaaggttta tcagtacacc accaatccgg atatttcttc
    81421 tggcgatggc attgctatgg cgtggcgcgc aggctgtcgg gttgccaatc tcgaatttaa
    81481 tcagttccac cctaccgcac tgtatcaccc acaggcacgc aatttcctgt taacggaagc
    81541 actgcgcggc gaaggcgctt atctcaagcg cccggatggc acgcgtttta tgcccgattt
    81601 tgatgagcgc ggcgaactgg ccccgcgcga tattgtcgcc cgcgccattg accatgaaat
    81661 gaaacgcctc ggcgcagatt gtatgttcct cgacatcagc cataagcccg ccgattttat
    81721 tcgccagcat ttcccgatga tttatgaaaa attgctcggg ctggggattg atctcacaca
    81781 agaaccggta ccgattgtgc ctgctgcaca ttatacctgc ggtggtgtaa tggttgatga
    81841 tcatgggcgt acggacgtcg agggcttgta tgccattggc gaggtgagtt ataccggctt
    81901 acacggcgct aaccgcatgg cctcgaattc attgctggag tgtctggtct atggctggtc
    81961 ggcggcagaa gatatcacca gacgtatgcc tgatgcccac ggcgtcagta cgttaccgcc
    82021 gtgggatgaa agccgcgttg agaaccctga cgaacgggta gtaattcagc ataactggca
    82081 cgagctacgg ctgtttatgt gggattacgt tggcattgtg cgcacaacga agcgcctgga
    82141 acgcgccctg cggcggataa ccatgctcca acaagaaata gacgaatatt acgcccattt
    82201 ccgcgtctca aataatttgc tggagctgcg taatctggta caggttgccg agttgattgt
    82261 tcgctgcgcg atgatgcgca aagaaagccg ggggctgcat tttacgctgg attacccgga
    82321 actgctcacg cattccggtc catcgatcct ttcccccggc aatcattaca taaacagata
    82381 aaaagcctgg gtcagcgccg tatacgcttc ggaatagttc tggtctggcc cacgaatgac
    82441 taagcgatcg ctaaagcatt ctcccgcctg cggggagaat gccagcagca cccgatgcgg
    82501 cagtcgcgct tcgttttccg ccacatccgt ccgcaaacgt aaatgccagc ccatgcttaa
    82561 tgccagttca gtaaaaccat taccaatctg ctctggcagc accacgcaga aaaatccctc
    82621 ttcggtaatg cactccgccg cacaggtcag cagcgatggg tgatcaagcg tagtggtata
    82681 gcgagcctgt tcccgttgag gtgtcgcgca ctctactccc tgctgatagt aaggtgggtt
    82741 actgatgatt aaatcgaagc gtactgtctg ctgtgtgatc cactgctgaa tatccgccgt
    82801 atggacgtta atccgctctg cccacgggga ctggttgata ttttcctgcg cctgcgcggc
    82861 agcttcactt tccagctcaa cggcatcaat tattacgctg tcactggttc gctgcgccag
    82921 catcagtgcc agcaacccgc tacccgcgcc gatatcaagg caacgtttca ccccagccac
    82981 cggtgcccat gcgcccagca aaatgccatc cgttcccact ttcatcgcac agcgatcgtg
    83041 agcaacaaaa aactgtttaa aagtaaatcc attacgacga agcacggatg tagactgtga
    83101 catgaaaata aaaccttgca ggaaaaacgg cgatagcacc gggtgagaac aatacccgag
    83161 aagcgatatc catacaaaca gatgaagatt gcagccgtaa cgtctataat cagcgcccca
    83221 cacagaggta gaacatgact gtaacgactt tttccgaact tgaactcgac gaaagcctgc
    83281 tggaagccct ccaggataaa ggtttcactc gcccgaccgc cattcaggct gccgccattc
    83341 cgcctgcgct cgatggccgt gatgtactcg gttctgcgcc gacaggcacc ggtaaaacgg
    83401 cggcgtatct gctgccagcg ttgcagcacc tgctcgattt cccgcgtaag aaatccggtc
    83461 cgccgcgtat tttgatcctc accccaactc gcgagctggc gatgcaggtg gccgatcatg
    83521 cccgcgaact ggcgaaacat acgcatctgg atatcgccac catcaccggc ggcgtagcct
    83581 atatgaacca cgcggaagtg ttcagcgaaa atcaggacat cgtggtcgcc acgaccggac
    83641 gtctgctgca atacataaaa gaagagaact tcgattgccg cgcggttgaa acgctgatcc
    83701 tcgacgaagc agaccgtatg ctggatatgg gcttcgctca ggatatcgaa catattgctg
    83761 gcgaaacgcg ctggcgtaaa cagaccctgc tcttttcggc aacgctggaa ggcgatgcga
    83821 ttcaggactt tgccgagcgt ctgctggaag atccggtgga ggtttctgcc aatccgtcca
    83881 cccgtgagcg caaaaaaatt catcagtggt attaccgcgc cgatgacctg gaacataaaa
    83941 ccgcattgct ggtgcatctg ttaaaacagc cggaagcgac ccgctcaatt gtgtttgtgc
    84001 gtaagcgtga acgtgtgcat gagctggcaa actggctgcg cgaagcgggc atcaacaact
    84061 gctatctcga aggtgagatg gtacaaggca agcgtaacga agcgatcaag cgtttgaccg
    84121 aaggtcgcgt aaacgtactg gttgcaaccg atgttgccgc gcgcggtatc gacattcctg
    84181 acgtcagcca cgtctttaac ttcgatatgc cgcgcagtgg cgatacttat ttgcaccgta
    84241 tcggacgtac cgcgcgcgcc ggtcgtaaag gcaccgcaat ttcgctggtg gaagcccatg
    84301 accatttgct gctgggcaag gttggccgtt acattgaaga gccgattaaa gctcgcgtta
    84361 ttgatgagtt acgcccgaaa acgcgtgcgc caagcgagaa gcagaccggc aagccatcga
    84421 agaaagtact ggcaaaacgc gccgagaaga aaaaagctaa agagaaagag aaaccgcgtg
    84481 tgaaaaaacg ccatcgcgac accaaaaata ttggtaagcg ccgtaaacca agcggaacgg
    84541 gcgtgccacc gcaaacgaca gaagagtaat ctcaatgccg ggtttaagcc tggtattaaa
    84601 agtgc
//
LOCUS       DAZGOB010000003        82546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGOB010000003 DAZGOB010000000
VERSION     DAZGOB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 82546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.003"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            214..840
                     /gene="ydhY"
                     /locus_tag="ACHLIF_000163"
     CDS             214..840
                     /gene="ydhY"
                     /locus_tag="ACHLIF_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO9604243.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            861..2963
                     /gene="ydhV"
                     /locus_tag="ACHLIF_000164"
     CDS             861..2963
                     /gene="ydhV"
                     /locus_tag="ACHLIF_000164"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO9604244.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2976..3614
                     /gene="ydhW"
                     /locus_tag="ACHLIF_000165"
     CDS             2976..3614
                     /gene="ydhW"
                     /locus_tag="ACHLIF_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO9604245.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3678..4346
                     /gene="ydhX"
                     /locus_tag="ACHLIF_000166"
     CDS             3678..4346
                     /gene="ydhX"
                     /locus_tag="ACHLIF_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9604246.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4343..5128
                     /gene="phsC"
                     /locus_tag="ACHLIF_000167"
     CDS             4343..5128
                     /gene="phsC"
                     /locus_tag="ACHLIF_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO9604247.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5132..5944
                     /gene="ydhT"
                     /locus_tag="ACHLIF_000168"
     CDS             5132..5944
                     /gene="ydhT"
                     /locus_tag="ACHLIF_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO9604248.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5956..7560)
                     /gene="ydhS"
                     /locus_tag="ACHLIF_000169"
     CDS             complement(5956..7560)
                     /gene="ydhS"
                     /locus_tag="ACHLIF_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO9604249.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDIGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7686..7991)
                     /gene="ydhR"
                     /locus_tag="ACHLIF_000170"
     CDS             complement(7686..7991)
                     /gene="ydhR"
                     /locus_tag="ACHLIF_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO9604250.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8099..8175)
                     /locus_tag="ACHLIF_000171"
     tRNA            complement(8099..8175)
                     /locus_tag="ACHLIF_000171"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8139..8141),aa:Val,seq:gac)
     gene            complement(8180..8256)
                     /locus_tag="ACHLIF_000172"
     tRNA            complement(8180..8256)
                     /locus_tag="ACHLIF_000172"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8220..8222),aa:Val,seq:gac)
     gene            8564..9820
                     /gene="ydhQ"
                     /locus_tag="ACHLIF_000173"
     CDS             8564..9820
                     /gene="ydhQ"
                     /locus_tag="ACHLIF_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO9604251.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9861..11234)
                     /gene="mdtK"
                     /locus_tag="ACHLIF_000174"
     CDS             complement(9861..11234)
                     /gene="mdtK"
                     /locus_tag="ACHLIF_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO9604252.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11449..12090
                     /gene="ribE"
                     /locus_tag="ACHLIF_000175"
     CDS             11449..12090
                     /gene="ribE"
                     /locus_tag="ACHLIF_000175"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO9604253.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12130..13278)
                     /gene="cfa"
                     /locus_tag="ACHLIF_000176"
     CDS             complement(12130..13278)
                     /gene="cfa"
                     /locus_tag="ACHLIF_000176"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO9604254.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13569..14780)
                     /gene="punC"
                     /locus_tag="ACHLIF_000177"
     CDS             complement(13569..14780)
                     /gene="punC"
                     /locus_tag="ACHLIF_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO9604255.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14893..15825
                     /gene="punR"
                     /locus_tag="ACHLIF_000178"
     CDS             14893..15825
                     /gene="punR"
                     /locus_tag="ACHLIF_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO9604256.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15822..16847)
                     /gene="purR"
                     /locus_tag="ACHLIF_000179"
     CDS             complement(15822..16847)
                     /gene="purR"
                     /locus_tag="ACHLIF_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO9604257.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17146..17235
                     /gene="cydH"
                     /locus_tag="ACHLIF_000180"
                     /gene_synonym="ynhF"
     CDS             17146..17235
                     /gene="cydH"
                     /locus_tag="ACHLIF_000180"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO9604258.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17401..18570
                     /gene="ydhP"
                     /locus_tag="ACHLIF_000181"
     CDS             17401..18570
                     /gene="ydhP"
                     /locus_tag="ACHLIF_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9604259.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18716..19297)
                     /gene="sodB"
                     /locus_tag="ACHLIF_000182"
     CDS             complement(18716..19297)
                     /gene="sodB"
                     /locus_tag="ACHLIF_000182"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO9604260.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19425..20252)
                     /gene="mepH"
                     /locus_tag="ACHLIF_000183"
     CDS             complement(19425..20252)
                     /gene="mepH"
                     /locus_tag="ACHLIF_000183"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO9604261.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20587..20934
                     /gene="grxD"
                     /locus_tag="ACHLIF_000184"
     CDS             20587..20934
                     /gene="grxD"
                     /locus_tag="ACHLIF_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO9604262.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20985..25601)
                     /gene="lhr"
                     /locus_tag="ACHLIF_000185"
     CDS             complement(20985..25601)
                     /gene="lhr"
                     /locus_tag="ACHLIF_000185"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO9604263.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQECSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVSDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECTARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25694..26341)
                     /gene="rnt"
                     /locus_tag="ACHLIF_000186"
     CDS             complement(25694..26341)
                     /gene="rnt"
                     /locus_tag="ACHLIF_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO9604264.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26444..26851)
                     /gene="gloA"
                     /locus_tag="ACHLIF_000187"
     CDS             complement(26444..26851)
                     /gene="gloA"
                     /locus_tag="ACHLIF_000187"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO9604265.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26932..28029)
                     /gene="nemA"
                     /locus_tag="ACHLIF_000188"
     CDS             complement(26932..28029)
                     /gene="nemA"
                     /locus_tag="ACHLIF_000188"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO9604266.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28066..28665)
                     /gene="nemR"
                     /locus_tag="ACHLIF_000189"
     CDS             complement(28066..28665)
                     /gene="nemR"
                     /locus_tag="ACHLIF_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO9604267.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28768..29007
                     /gene="ydhL"
                     /locus_tag="ACHLIF_000190"
     CDS             28768..29007
                     /gene="ydhL"
                     /locus_tag="ACHLIF_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO9604268.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29056..29952
                     /gene="ydhF"
                     /locus_tag="ACHLIF_000191"
     CDS             29056..29952
                     /gene="ydhF"
                     /locus_tag="ACHLIF_000191"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO9604269.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30033..30554
                     /gene="sodC"
                     /locus_tag="ACHLIF_000192"
     CDS             30033..30554
                     /gene="sodC"
                     /locus_tag="ACHLIF_000192"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO9604270.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30555..32567)
                     /gene="ydhK"
                     /locus_tag="ACHLIF_000193"
                     /pseudo
     CDS             complement(30555..32567)
                     /gene="ydhK"
                     /locus_tag="ACHLIF_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(32567..33424)
                     /gene="ydhJ"
                     /locus_tag="ACHLIF_000194"
     CDS             complement(32567..33424)
                     /gene="ydhJ"
                     /locus_tag="ACHLIF_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO9604271.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33427..33663)
                     /gene="ydhI"
                     /locus_tag="ACHLIF_000195"
     CDS             complement(33427..33663)
                     /gene="ydhI"
                     /locus_tag="ACHLIF_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO9604272.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33864..34298
                     /gene="slyA"
                     /locus_tag="ACHLIF_000196"
     CDS             33864..34298
                     /gene="slyA"
                     /locus_tag="ACHLIF_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO9604273.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34345..34812)
                     /gene="slyB"
                     /locus_tag="ACHLIF_000197"
     CDS             complement(34345..34812)
                     /gene="slyB"
                     /locus_tag="ACHLIF_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO9604274.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35086..36195
                     /gene="anmK"
                     /locus_tag="ACHLIF_000198"
     CDS             35086..36195
                     /gene="anmK"
                     /locus_tag="ACHLIF_000198"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO9604275.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36293..36622
                     /gene="mliC"
                     /locus_tag="ACHLIF_000199"
     CDS             36293..36622
                     /gene="mliC"
                     /locus_tag="ACHLIF_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO9604276.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36681..37337
                     /gene="pdxH"
                     /locus_tag="ACHLIF_000200"
     CDS             36681..37337
                     /gene="pdxH"
                     /locus_tag="ACHLIF_000200"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO9604277.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37466..38740
                     /gene="tyrS"
                     /locus_tag="ACHLIF_000201"
     CDS             37466..38740
                     /gene="tyrS"
                     /locus_tag="ACHLIF_000201"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO9604278.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38799..39662
                     /gene="pdxY"
                     /locus_tag="ACHLIF_000202"
     CDS             38799..39662
                     /gene="pdxY"
                     /locus_tag="ACHLIF_000202"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO9604279.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39706..40311)
                     /gene="gstA"
                     /locus_tag="ACHLIF_000203"
     CDS             complement(39706..40311)
                     /gene="gstA"
                     /locus_tag="ACHLIF_000203"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO9604280.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40417..41919)
                     /gene="dtpA"
                     /locus_tag="ACHLIF_000204"
     CDS             complement(40417..41919)
                     /gene="dtpA"
                     /locus_tag="ACHLIF_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO9604281.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42530..43165)
                     /gene="nth"
                     /locus_tag="ACHLIF_000205"
     CDS             complement(42530..43165)
                     /gene="nth"
                     /locus_tag="ACHLIF_000205"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO9604282.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     LVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43165..43860)
                     /gene="rsxE"
                     /locus_tag="ACHLIF_000206"
     CDS             complement(43165..43860)
                     /gene="rsxE"
                     /locus_tag="ACHLIF_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO9604283.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43864..44484)
                     /gene="rsxG"
                     /locus_tag="ACHLIF_000207"
     CDS             complement(43864..44484)
                     /gene="rsxG"
                     /locus_tag="ACHLIF_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO9604284.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44488..45546)
                     /gene="rsxD"
                     /locus_tag="ACHLIF_000208"
     CDS             complement(44488..45546)
                     /gene="rsxD"
                     /locus_tag="ACHLIF_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO9604285.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45547..47673)
                     /gene="rsxC"
                     /locus_tag="ACHLIF_000209"
     CDS             complement(45547..47673)
                     /gene="rsxC"
                     /locus_tag="ACHLIF_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO9604286.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQSNAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDP
                     RKAAVAAAIARAQAKKAAQQKVVNED"
     gene            complement(47666..48244)
                     /gene="rsxB"
                     /locus_tag="ACHLIF_000210"
     CDS             complement(47666..48244)
                     /gene="rsxB"
                     /locus_tag="ACHLIF_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO9604287.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48244..48825)
                     /gene="rsxA"
                     /locus_tag="ACHLIF_000211"
     CDS             complement(48244..48825)
                     /gene="rsxA"
                     /locus_tag="ACHLIF_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO9604288.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGHAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48902..49342)
                     /gene="ydgK"
                     /locus_tag="ACHLIF_000212"
     CDS             complement(48902..49342)
                     /gene="ydgK"
                     /locus_tag="ACHLIF_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO9604289.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49428..49643)
                     /gene="ydgT"
                     /locus_tag="ACHLIF_000213"
     CDS             complement(49428..49643)
                     /gene="ydgT"
                     /locus_tag="ACHLIF_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO9604290.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(49916..50041)
                     /gene="blr"
                     /locus_tag="ACHLIF_000214"
     CDS             complement(49916..50041)
                     /gene="blr"
                     /locus_tag="ACHLIF_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO9604291.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            50284..51324
                     /gene="ydgJ"
                     /locus_tag="ACHLIF_000215"
     CDS             50284..51324
                     /gene="ydgJ"
                     /locus_tag="ACHLIF_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO9604292.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51359..52360)
                     /gene="add"
                     /locus_tag="ACHLIF_000216"
     CDS             complement(51359..52360)
                     /gene="add"
                     /locus_tag="ACHLIF_000216"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO9604293.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(52464..53636)
                     /gene="malY"
                     /locus_tag="ACHLIF_000217"
     CDS             complement(52464..53636)
                     /gene="malY"
                     /locus_tag="ACHLIF_000217"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO9604294.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53646..55238)
                     /gene="malX"
                     /locus_tag="ACHLIF_000218"
     CDS             complement(53646..55238)
                     /gene="malX"
                     /locus_tag="ACHLIF_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO9604295.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVLALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55413..56441
                     /gene="malI"
                     /locus_tag="ACHLIF_000219"
     CDS             55413..56441
                     /gene="malI"
                     /locus_tag="ACHLIF_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO9604296.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56553..57320
                     /gene="hdhA"
                     /locus_tag="ACHLIF_000220"
     CDS             56553..57320
                     /gene="hdhA"
                     /locus_tag="ACHLIF_000220"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO9604297.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57541..58131
                     /gene="uidR"
                     /locus_tag="ACHLIF_000221"
     CDS             57541..58131
                     /gene="uidR"
                     /locus_tag="ACHLIF_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO9604298.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAERMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            58520..60331
                     /gene="uidA"
                     /locus_tag="ACHLIF_000222"
     CDS             58520..60331
                     /gene="uidA"
                     /locus_tag="ACHLIF_000222"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO9604299.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     AEQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            60328..61701
                     /gene="uidB"
                     /locus_tag="ACHLIF_000223"
     CDS             60328..61701
                     /gene="uidB"
                     /locus_tag="ACHLIF_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO9604300.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            61740..63005
                     /gene="uidC"
                     /locus_tag="ACHLIF_000224"
     CDS             61740..63005
                     /gene="uidC"
                     /locus_tag="ACHLIF_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO9604301.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(63050..64558)
                     /gene="ydgA"
                     /locus_tag="ACHLIF_000225"
     CDS             complement(63050..64558)
                     /gene="ydgA"
                     /locus_tag="ACHLIF_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO9604302.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(64659..65834)
                     /gene="manA"
                     /locus_tag="ACHLIF_000226"
     CDS             complement(64659..65834)
                     /gene="manA"
                     /locus_tag="ACHLIF_000226"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO9604303.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSILQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            66033..67679
                     /gene="fumB"
                     /locus_tag="ACHLIF_000227"
     CDS             66033..67679
                     /gene="fumB"
                     /locus_tag="ACHLIF_000227"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO9604304.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            67822..69225
                     /gene="fumC"
                     /locus_tag="ACHLIF_000228"
     CDS             67822..69225
                     /gene="fumC"
                     /locus_tag="ACHLIF_000228"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO9604305.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(69222..70151)
                     /gene="tus"
                     /locus_tag="ACHLIF_000229"
     CDS             complement(69222..70151)
                     /gene="tus"
                     /locus_tag="ACHLIF_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO9604306.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(70227..71528)
                     /gene="rstB"
                     /locus_tag="ACHLIF_000230"
     CDS             complement(70227..71528)
                     /gene="rstB"
                     /locus_tag="ACHLIF_000230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO9604307.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(71532..72251)
                     /gene="rstA"
                     /locus_tag="ACHLIF_000231"
     CDS             complement(71532..72251)
                     /gene="rstA"
                     /locus_tag="ACHLIF_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO9604308.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            72380..72715
                     /gene="ydgC"
                     /locus_tag="ACHLIF_000232"
     CDS             72380..72715
                     /gene="ydgC"
                     /locus_tag="ACHLIF_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO9604309.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(72712..73434)
                     /gene="folM"
                     /locus_tag="ACHLIF_000233"
     CDS             complement(72712..73434)
                     /gene="folM"
                     /locus_tag="ACHLIF_000233"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO9604310.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(73471..74853)
                     /gene="ydgI"
                     /locus_tag="ACHLIF_000234"
     CDS             complement(73471..74853)
                     /gene="ydgI"
                     /locus_tag="ACHLIF_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9604311.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(75039..75983)
                     /gene="ydgH"
                     /locus_tag="ACHLIF_000235"
     CDS             complement(75039..75983)
                     /gene="ydgH"
                     /locus_tag="ACHLIF_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO9604312.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            76507..78039
                     /gene="pntA"
                     /locus_tag="ACHLIF_000236"
     CDS             76507..78039
                     /gene="pntA"
                     /locus_tag="ACHLIF_000236"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO9604313.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            78050..79438
                     /gene="pntB"
                     /locus_tag="ACHLIF_000237"
     CDS             78050..79438
                     /gene="pntB"
                     /locus_tag="ACHLIF_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO9604314.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(79463..80368)
                     /gene="tqsA"
                     /locus_tag="ACHLIF_000238"
                     /pseudo
     CDS             complement(79463..80368)
                     /gene="tqsA"
                     /locus_tag="ACHLIF_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(80375..80557)
                     /locus_tag="ACHLIF_000239"
     CDS             complement(80375..80557)
                     /locus_tag="ACHLIF_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9604315.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            80545..81774
                     /locus_tag="ACHLIF_000240"
     CDS             80545..81774
                     /locus_tag="ACHLIF_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9604316.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNVLDMWMERLGI
                     LAGTHENVTTLPVARRK"
     gene            82149..82337
                     /locus_tag="ACHLIF_000241"
     CDS             82149..82337
                     /locus_tag="ACHLIF_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9604317.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            82395..>82546
                     /locus_tag="ACHLIF_000242"
     CDS             82395..>82546
                     /locus_tag="ACHLIF_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9604318.1"
                     /translation="MKIEYTPERGRGFVRPGETEKQQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCV"
BASE COUNT        20881 a        20279 c        21478 g        19908 t
ORIGIN      
        1 gataacgatc aatgttaagg gctcagaata atcacattat tttttctcct aaccataaag
       61 gattagttta tcggcgttaa atcttttctc atacactatc gggttaggga aaatatctgg
      121 tgttggggac agagtcagca gcgtattagt tgaatgctta atatcaggct gctgaattgt
      181 cagcactgtg tttgcgaaga ggatgcgaaa taaatgaacc cggttgatcg tccactatta
      241 gatattggct taacgcgatt agaattcttg cgtatatcag gaaaaggcct tgctggtcta
      301 acaattgccc ctgcgttact gtcacttttg ggttgcaaac aggaagatat tgatagcggc
      361 acagtagggt tgataaatac acccaaaggg gtgctggtga cccagcgagc gcgatgcacg
      421 ggttgccacc gctgtgaaat ctcttgtacc aacttcaacg atggctcagt agggacattc
      481 ttctcccgta tcaaaatcca ccgcaattat ttctttggcg acaacggggt tggctctggc
      541 ggcggcctgt atggcgatct caactatacc gcggacacct gccgtcaatg caaagagccg
      601 caatgcatga acgtctgccc gattggtgcg attacctggc agcagaaaga aggctgtatt
      661 accgtcgatc ataaacgctg tattggctgt agcgcctgta ctacggcatg cccgtggatg
      721 atggccaccg taaataccga aagtaaaaaa tcctcgaaat gtgtgttatg cggtgaatgc
      781 gcaaacgcct gcccaacagg ggcgttaaaa attatcgagt ggaaagatat tactgtgtga
      841 atcttgcaaa ggaaaaaatc atggctaacg gttggacagg taatatatta agagtcaatc
      901 tcacgacagg aaatattacc ctcgaagatt ccagtaagtt taaaagtttt gtcggtggca
      961 tgggcttcgg ctacaaaatt atgtatgacg aagtaccgcc aggcacgaaa cctttcgatg
     1021 aagcgaataa attagtcttt gctaccggcc cattaactgg atctggtgcc ccctgtagtt
     1081 ctcgcgtaaa tatcacctca ctttctactt ttaccaaagg aaatttagtt gtcgatgccc
     1141 atatgggtgg cttttttgca gcgcaaatga aattcgctgg atacgacgtc attattatcg
     1201 aagggaaggc gaaatcaccg gtatggctga agattaaaga tgacaaagtt agcctggaaa
     1261 aagccgattt cttatgggga aaagggacgc gcgcaacgac ggaagaaatt tgtcgattga
     1321 ccagtccgga aacctgtgtg gcggctattg gtcaggctgg ggaaaacctt gttcctctct
     1381 ctggcatgtt gaatagccgt aaccacagcg gcggtgcggg aactggcgca attatgggtt
     1441 cgaaaaacct gaaagcgatt gcggttgaag ggacgaaagg ggtcaacatt gccgatcgtc
     1501 aggagatgaa gcgtctcaac gattacatga tgactgaact tattggtgcg aataacaacc
     1561 atgtcgtgcc aagtacgccg caatcgtggg cagagtattc agatcccaag tcacgttgga
     1621 cagcacgtaa agggctgttt tggggcgcgg ctgaaggtgg tccgattgaa acgggggaaa
     1681 ttccgccagg caatcagaac acggtcggct ttcgtaccta taaatccgtt tttgacttag
     1741 gtccggcggc agagaaatac acagtaaaaa tgagtggctg ccactcttgc ccgatccgtt
     1801 gtatgaccca aatgaatatt cctcgggtga aagagtttgg cgtgcccagc acaggtggta
     1861 acacttgtgt agctaacttt gtccatacca ccatcttccc gaacgggccg aaagattttg
     1921 aagataaaga cgatggtcgt gtgattggta acctggttgg tctgaatttg ttcgatgact
     1981 acggcctatg gtgtaactac gggcagctgc atcgcgactt tacttattgt tacagcaaag
     2041 gggtgttcaa gcgtgttctg ccagctgaag agtatgcgga aattcactgg gatcaactgg
     2101 aagcgggtga cgttaatttc attaaagatt tttactaccg tctggcgcat cgtgtgggtg
     2161 agctgagtca cctggctgat ggttcatatg ccatcgcaga acgctggaat ttgggtgaag
     2221 agtactgggg ctacgcaaaa aataaactct ggtcgccgtt tggctatccg gttcaccatg
     2281 ccaatgaagc gtcagcgcag gtcggttcca ttgttaactg tatgttcaac cgtgactgca
     2341 tgacgcatac ccatatcaat tttattggtt cgggtttgcc attgaaactg cagcgtgaag
     2401 tggcgaaaga gctttttggt tctgaagatg cctacgatga aaccaaaaac tacacgccaa
     2461 tcaatgacgc aaaaattaaa tatgcaaagt ggtcgctgtt gcgggtctgt ttgcataacg
     2521 ccgtcaccct gtgcaactgg gtctggccaa tgaccgtttc gccgctgaaa agccgtaatt
     2581 accggggcga tctggcgctt gaagccaaat tcttcaaagc gatcaccggc gaagagatga
     2641 ctcaggaaaa attagattta gctgcagagc gtatctttac gttgcatcgt gcctacacgg
     2701 taaaactgat gcaaaccaaa gatatgcgta atgaacacga tcttatctgt tcctgggtat
     2761 tcgacaagga tccgcagatc ccggtcttta ctgaaggtac tgacaaaatg gatcgtgacg
     2821 atatgcatgc ttcgctcacg atgttctaca aagaaatggg ctgggaccca cagcttggtt
     2881 gtccgacccg cgaaacattg cagcgtctgg ggctggaaga tattgccgcc gatctggcag
     2941 cacacaatct actgcctgtg taaggaatgg gtgagatgaa ccatcgggat gaattgccgt
     3001 tagcgaaagt cagcgaagtt gacgaggcga agcggcaatg gttacagggg atgcgtcacc
     3061 ccgttgatac ggtaaccgaa ccagaaccgg ctgaaatcct ggcagagttc attcgccagc
     3121 attctgcggc tgggcagctg gtggcgcgcg ccgtgtttct ctcgccaccc tattcggttg
     3181 cagaagagga gctgtcagtt ttgctggaaa gcattaagca gaacggagac tacgcggata
     3241 tcgcttgtat gacgggaagc caggatgatt actattactc aacgcaggcc atgagtgaaa
     3301 actacgcggc gatgtctttg caagttgtgg agcaggacat ttgtcgcgcc attgcacacg
     3361 ccgttcgttt tgagtgccag acatatcctc gtccttacaa agtcgccatg ctgatgcaag
     3421 cgccatatta cttccaggaa gcgcagattg aagccgcgat cgccgcgatg gatgttgcac
     3481 ctgaatatgc cgatatacgg caggtggagt catcgacggc ggtgttgtac ttgtttagcg
     3541 agcgatttat gacctatggc aaggcttatg gcttatgcga atggttcgag gtagagcagt
     3601 ttcagaaccc ctgaaatttc agggagatgt catggatagg gtggacagtt gcagccactg
     3661 cattagggga taatcagatg tccttcactc gacgcaaatt tgttctgggg atgggaacag
     3721 taatcttttt tactggttct gcgtcttctc tgttagcgaa cacgaggcaa gaaaaggaag
     3781 ttcggtatgc catgattcat gacgagtcac ggtgcaatgg ctgtaatatt tgcgcccgcg
     3841 catgccgtaa aactaaccac gtccccgctc agggaagccg tttatcgata gcacacattc
     3901 cagtcaccga taacgacaac gaaacgcagt atcacttttt tcgccagtca tgtcagcact
     3961 gcgaagatgc tccgtgcatc gacgtatgcc cgacaggggc gtcgtggcgt gatgagcagg
     4021 ggatcgtgcg ggtagaaaaa tcgcagtgca tcggctgcag ttattgcatt ggcgcttgtc
     4081 cgtaccaggt tcgctacctt aaccctgtca ctaaagtggc agataaatgt gatttctgcg
     4141 ctgagtcccg actggcaaaa ggctttccgc ccatttgcgt cagcgcctgc ccggaacacg
     4201 cattgatctt tggtcgcgaa gatagcccag aaattcaggc gtggttgcag caaaataaat
     4261 actatcaata tcagctacct ggtgccggga aaccgcatct gtatcgccgg ttcggtcaac
     4321 atttgattaa aaaggaaaat gtatgaaccc gtcgcaacat gctgaacagt ttcagagcca
     4381 gttagcgaac tatgtgccac agttcactcc cgaattttgg ccggtgtggt tgatcattgc
     4441 cggagtattg ctggttggga tgtggttggt gctggggctg catgccttgc ttcgtgctcg
     4501 tggcgtgaag aaatcagtca ccgattatgg tgagaagatt tatctttact gcaaagcggt
     4561 cagattatgg cactggtcga atgcgttact ctttgtattg ttgctggcaa gtgggctgat
     4621 aaatcacttt gcgctggtgg gcgcaactgc ggttaaaagt ctggttgcgg tgcatgaagt
     4681 ttgcggattt ttgttactcg catgctggct cggctttgtg ctgatcaatg ccgttggggg
     4741 taatggtcac cactatcgca ttcgtcgtca ggggtggctg gagcgagcgg caaaacaaac
     4801 gcgattttat ttgtttggca ttatgcaggg ggaagaacat cctttcccgg caacaaccca
     4861 gtctaaattt aatcccttac agcaggtcgc ctatgttggt gttatgtatg gattgctgcc
     4921 gttgttacta ttgacggggc tgctgtgtct ctatccgcaa gccgtgggag atgtgtttcc
     4981 tggcgtaaga tactggttat tgcaggcgca ttttgctctg gcatttataa gcctcttttt
     5041 tatcttcggt catctttatc tttgcaccac ggggcgtacg ccacacgaaa cctttaaaag
     5101 catggtcgat ggctatcacc ggcactaacg aatgatcatt acacgagcag acctgcgaga
     5161 atggcgtatc ggagcggtga tgtatcgctg gttcctgcgc catttcccac gcggcggtag
     5221 ttatgccgat attcatcatg cactgattga ggaaggatat acggactggg cggagagtct
     5281 ggttgagtat gcgtggaaaa aatggctggc ggatgaaaac ttcgcgcatc aggaagtttc
     5341 ctctatgcaa aaactggcca cagaccccgg agagatacca ttttgcagtc agtttgcccg
     5401 cagcgacgat catgctcgca tcggctgttg tgaggataac gcccgaatcg caaccgctgg
     5461 gtatgccgcg caaattgcca gtatgggtta tagcgtgcgg attggaagtg tcggctttaa
     5521 cagtcacatt ggcagctccg gtgaacgcgc acgggttgct gtaaccggta attcttcgcg
     5581 gataagcagc gctggagata gtagtcggat tgcgaatacc ggaatgcgag tacgcgtttg
     5641 tacattgggt gaacggtgtc atgtcgccag taatggtgac ctggtgcaaa tcgccagctt
     5701 tggcgcaaac gccagaattg ccaacagcgg cgacaatgta cacatcattg ccagcggtga
     5761 aaattcaacg gtagtgagca cgggcgtggt cgactcaatt attctcgggc cagggggaag
     5821 cgccgcactg gcatatcatg acggcgaaag agttcgcttc gccgtcgcca ttgaaggtga
     5881 aaacaatatt cgaaccggag tccgctatcg tcttaatgaa cagcatcagt ttgttgagtg
     5941 ctgatagctt tacctttaat caaacgaaag acgccgacga gcacgggatg caggcttcac
     6001 gaccgcccga gccatcgcct caccaatttc tgcacatgcc gtaagtccct gaacgaaagg
     6061 ctggtcgtgc atcaaccagg gcaacgcgcc gaacgctacg cgtccacgaa tatcttcggg
     6121 ttgctgtaac gtataatctt cgccaatatc agggatttca tcccctgttt tctgtaattg
     6181 ctcgcgtagc ccagggaaag gaatatcttt cactttaagc ggacgctgtc cgcgggcatc
     6241 aataaaaacg tcaaacgagt agctgttgtc ttccgttttc aggacggtgc gcgactcatt
     6301 aatttccatt ttgtagtctt caccgagggc gagaatatga atgattcccg cttcacgtaa
     6361 ggcaagtagg cgacgaatag actctgaagg gattgccgca taattatcga tgaaaaccct
     6421 ggcaaggcct ttactgaacc gtttatggtc gtgttcattc agatgaggaa caatttcctg
     6481 tacggcttca tgcaggcgca gaatgacata acgccaggga acagtatgtt tttctcgttt
     6541 attgcgttcg acttcctgga gatttttttc tgcccagtca aatgggtcgc gttgtttgcg
     6601 ctcggcaaac caggcttgag caaaggaatc gacattcagg ctctctaagg ctatgcgttg
     6661 gctccagtct ggatcagcaa acttgatttc ctctactatc aatctaaata cccgatccaa
     6721 aaggccctct tcaccttttt gaatctcagc atttaatgcc tgatcggtga cgatgtgtaa
     6781 gggctcgtag ggaatagggc aatagaaatc ggcttcgggt aaaatacccg tgcgcgacat
     6841 caacgtaata tttagctttt cacttgcgtt atcgcggtta aaaacgacgt gttgtttatc
     6901 atcttcaatg aacgaaccat gctgaatagc cactgccatt gccgcatcca gtccgctcaa
     6961 ggatgttccc ataataccca cgttacacgc atcgaccttt gcttccatca ggcctgacca
     7021 cggactggga aaatacgttc tggttgcttc ttcttcatca ggccagacgt gacccgtggc
     7081 aatcaccgct aaatcaaacg tctcgctggg taaatcctga tttgtagcga gcatgacgcc
     7141 agcatttgta atttgcagat cggtaacctg gcatgattca taaacagcca ctgcaaattt
     7201 ttgctgtcgt gcctggtcta ctaatcgtaa aaattgatcg cggaaatatt cgcccagcag
     7261 aattcgcggt aaaaactgac gatcgtgcaa ggtttctttt ttaacgccat aacgctggag
     7321 atggctggct tcttgctttt gtagccattc gagatacgta caattaatcg gcggtatttc
     7381 aatactggca atatttgcca gcatcatttt tgagttttcc tcatcactgt atggcatccc
     7441 gacaccggcc tcgtcagcct gctcgaagat agaaatagaa agtggagttt gttgctgtag
     7501 aagcgagaat aaggtgtaga tccccgtagg cccggcaccc acaatagcaa tttttttcat
     7561 tgttacttac ctgtacgcat tctttcgaat ttagtgttat cagataagtt tggttgagat
     7621 ttttagaatt tgagaaattt cagacaggta tgaaaagggc gctaaccgcc cttgattaaa
     7681 ttctgtcagg cgagttttgc ctgattgatt tgactcagtg gctcattgac atcgaaaact
     7741 ttggcgacaa cttcctcaac gccgagattt ttaagtcggg cagtatgttt ttccagatag
     7801 gcaagggcgc ttttttcatc agtaaaaaga tagatcccac cggcttcgtg gttcttttca
     7861 ctttctgtcc ataccttcca cagaaaacca ggttcctgat taatcgactc agcaagtggt
     7921 ttaagctgct cagccattgc gtcgccaaat gggccattaa aagcaaaatg aagttgtaac
     7981 aaagttgcca tagcaattct ccttctgata acgcatacga agacagtgta aatcttttaa
     8041 cgtattggat acagagaaga aacagaggaa aaaaggagaa gaccagatta agaaaatctg
     8101 gtgcgtccga gtggactcga accaccgacc cccaccatgt caaggtggtg ctctaaccaa
     8161 ctgagctacg gacgcaggat ggtgcgttca attggactcg aaccaacgac ccccaccatg
     8221 tcaaggtggt gctctaacca actgagctat gaacgcaacg ttgtaggtga caacggggac
     8281 gaatattagc ggcagagtgg gaaggtggca agaggcaaaa cgtaattttc tgcgctattt
     8341 cgaccgtttg cagagctttt aagcaaattg gctatatttt gttgatttgc aagggcgatt
     8401 tttattcagg atcgcattta catctgatac aacccaataa ttcgtgtatc agagtttaaa
     8461 cagagttgtt tgccaccatc tcaaatctgt cgtggataaa tcctccgtag cctttacact
     8521 taggtagcaa taaaggcaca cagatgcagc aaaggaataa atcatgggat ctgatgcgaa
     8581 aaacttgatg agcgacggga atgtgcaaat tgttaagacc ggcgaggtca ttggcgcgac
     8641 gcaacttact gaaggcgagt tgattgttga agctggcgga agagccgaaa ataccgtggt
     8701 cacgggggct ggctggttga aagtggcaac cggtgggatc gccaaatgca cacagtacgg
     8761 taacaatggc acgctatcgg tcagcgacgg tgccattgcc acagatattg ttcagtccga
     8821 gggaggcgca attagtctct ctacgctcgc tacggttaat ggccgccatc ccgaaggtga
     8881 attcagcgtt gatcagggtt atgcctgcgg tttgttgctg gaaaatggcg gtaacctgcg
     8941 tgtactggaa ggacatcgcg cggaaaaaat cattctcgat caagagggcg gcctgttggt
     9001 caatgggaca acctcagctg tcgtggtaga tgaaggtggt gaattgttgg tgtatccagg
     9061 tggggaagcc agcaattgtg aaattaatca gggcggcgtt tttatgctgg ccgggaaagc
     9121 cagtgatacg ttgcttgctg gtggcaccat gaataatctc ggtggtgaag actctgacac
     9181 tattgttgag aatggatcca tttatcgtct ggggacggat ggccttcagc tctacagttc
     9241 cggtaagacg caaaacctgt ccgtgaatgt gggtggtcgg gctgaagtgc atgccggtac
     9301 gctggaaaat gcggtaatac aaggtggaac agtgatcctg ttgtcaccca ccagcgcgga
     9361 cgaaaatttt gtcgtagagg aagatcgcgc accggttgaa ctgaccggga gtgttgcatt
     9421 actggacggc gcttcaatga ttattggcta tggtgcagat ctgcaacaat caacgattac
     9481 tgtacagcag ggcggtgtgt tgattctcga cggcagtacg gtaaaaggtg acagtgtcac
     9541 ttttagtgtt ggtaacatca atctgaatgg cggaaaactg tggctgatca ctggtgcggc
     9601 aacgcatgtg caactgaaag tgaaacgcct gcgcggagag ggagcgattt gcctgcaaac
     9661 cagtgcgaaa gaaatctcac ctgacttcat caatgtgaaa ggggaagtta ccggggatat
     9721 acgcgttgag ataacagatg ccagtcggca aactctgtgt aacgcactga aactacagcc
     9781 agacgaagac gggattggcg caacgctcca gcctgcgtaa aaacacaggc gcaacccgtg
     9841 aaggttgcgc cttgtcttta ttagcgggac gctcgttgca gaatgatggc tgacggcaga
     9901 cgttgcagga accgcatacg cagcatcatc ataatggctg ccgacgtcag gccaataata
     9961 aagcctatcc agaagcctgc tggccccata ggttcaacga ccagatcggt cagtgccaga
    10021 atatagccgc ttggcaagcc cagcacccag taagccgtaa aggtaatata gaaaatggaa
    10081 cgcgtatctt tataaccacg caaaatccca ctgccaatca cctggatcga gtcagaaatc
    10141 tgatataccg ccgccagcaa catcaaatgc gcagccagcg ttacaacctc gggattgtcg
    10201 ttgtacaaca gggcgatttg ctcccgcagt gaaaccgtga atatggccgt cagggttgcc
    10261 atacagacac ccaccataag cccggtccgc gcagcggttt gcgcatccag cgttgagccc
    10321 tgacccagac gataacctac gcggatagtt accgctgccg ccagcgacat gggaagcacg
    10381 aacattagtg aactaaagtt cagggcaatc tggtgtcctg cgacatcaac aataccgagc
    10441 ggagacacta acagagccac gacagcaaac agtgtcactt caaagaacag tgccagcgca
    10501 atcggcaaac cgagttgaat cagtcgtttc ataaccgcag gatcgggttt cgcggtgcct
    10561 ttttcgttac gaatatcgcg catggagcgg gcgcgtttaa tgtaagaaac catggcaagg
    10621 aacataaccc agtacaccgc cgcagtagcc acgccacaac caacgccacc gagctcaggc
    10681 ataccgaaat gaccataaat aaagatatag ttcaccggaa tgttcaccag caggccgata
    10741 aagcccatta ccatacccgg cttggttttt gccagacctt cacactggtt acgggcaacc
    10801 tggaagaaca gatagcccgg cgcgccccac aacaacgcac gcagataacc cacggctttg
    10861 tccgccagag ccggatcgat gttttccatg gagcggataa tgtaacctgc attccacagc
    10921 accagcataa tgagaacgga aacaaaacct gccagccaga aaccttgtcg cacctgatgc
    10981 gcaatgcgct cacgtcgacc ggaaccattt aattgcgcga taaccggcgt taatgccagc
    11041 agcagtccgt gaccaaagag gatcgccgga agccagatag aagtaccgat agcgaccgcc
    11101 gccatgtcgg tggcactata gccgcccgcc atcacggtat cgacaaaacc catcgcagtt
    11161 tgggcgattt gcgcgagaat caccgggatt gctaatgcta ataacagacg cgcttcactg
    11221 atatacttct gcacgtgaac accttttatt tgtagttata tgagagacta aaaagccgcc
    11281 tgaatgggca gcaaaaagaa gaagcagggg aaattcagtc tattgtagcg aggatttatt
    11341 aattctccag tgaaaaaata gtcattctta ccccttcact ggtaagggcg tttgccacct
    11401 gctattgtgc tgaacagaat gtcttaactg atttcaggag ttgtaagtat gtttacgggg
    11461 attgtacagg gcaccgcaaa actggtgtcg attgacgaga aaccaaattt tcgtacgcat
    11521 gtggtggagt tacccgacca catgctggac ggcctggaaa ccggtgcgtc cgtggcgcat
    11581 aacggttgct gcctgaccgt gacggaaatt aacggcaacc atgtcagttt tgacctgatg
    11641 aaagaaacgt tacgcattac caatcttggc gatttaaaag tgggggattg ggtaaacgtt
    11701 gagcgtgcgg cgaaattcag tgatgaaatt ggcggacact taatgtcagg tcatattatg
    11761 accactgctg aagtggcgaa aatattaacc tcagaaaata atcgccagat ctggtttaaa
    11821 gtccaggata gtcagttgat gaaatatatt ctgtacaaag gatttattgg catcgacggt
    11881 attagcctga ccgtcggcga agtcacgcca acgcgttttt gcgtccattt aattccggaa
    11941 acactggaac gcacgactct tgggaagaaa aaactcggcg cacgcgtcaa cattgaaatt
    12001 gatccacaaa ctcaggcagt ggtagatacg gtagaacgtg tgctggcggc acgagaaaat
    12061 gccatgaatc aaccaggtac agaagcctga tctgaactcg cccccgaaga gggggcgata
    12121 gaatagcctt tagcgagcga ctcgaaggcc gttttcaaca ccgcgtgaga acaccacctg
    12181 ccagagctga atatcacggg cgcggaaagc acctgcacaa gcattcagat aataggtaaa
    12241 cattcgttta aagcgttcac tgtagttatc tgcaatttct ggccatgcgg cgaggaatcg
    12301 ttcataccac gccatcaacg tagtatcgta atcagcaccg aagttatgcc agtcttccat
    12361 cacaaagtgg ggttcgctgg actgagcaat ctggcgtaca gagggcaggc aaccgttcgg
    12421 aaaaatatat ttattaatcc agggatcaac attcagatcg gtttttttcg aaccgatagt
    12481 atggagcagg aatatacctt ccggtttcaa attacgatcc accaccgcaa aataggtatc
    12541 gtaatttttc ggtccgacgt gctcgaacat ccccacagaa acaatacgat caaactggtc
    12601 gttcaggtca cgataatctt gcagcaaaat ggtgacatcc aggccttcac agcgttcctg
    12661 agccattttt tgctgttcgg cagaaatggt gacgccgacc acgcttacgt catagttaga
    12721 tgccatgtag tgcgccagtc cgccccagcc gcagccaata tccagtacgc gcatccctgg
    12781 ttttaactgc aatttttcac aaatcatttt gagcttcgcc tgctgggcag attccagatt
    12841 atcggcatct ttccagtaag cgcaggaata ttgcatgaag ggatcaagca tgcgactgaa
    12901 caagtcatta cccaaatcgt aatgctcttt gccgactatc caggcacgtt ttttactctg
    12961 cagattgaag agacgggcgc ctgcaatacg cagcgtgtct ttgaaatgat gggggagttg
    13021 gttctcgaga cctgcgcgta agactttgct aaaaaacata tccagtcggt cacattccca
    13081 ccagccatcc atataacttt cgcctaaccc caaagagcct tcttgcagaa cgcgtttaaa
    13141 aaaatcgggg tttttcacac gaatatccgc cggggcagaa ccgttaatgg ctataccggc
    13201 acggctaagt aattcgttgg cgatacggta ccagttgtca tccggtacac tgacttcttc
    13261 tatacacgat gaactcatag tttctccatc actggtcggt gatcagaacc ttaaaacagc
    13321 gtagacgctt tttgggcttt gtgagaaatc tcgcagaaaa aaccgcgagt ctataatccg
    13381 acgtagaaca gagggaaagg aggaaccctt gccgaaatga ccatccatag tgagtcggga
    13441 acgatcctgt ccccgtaaaa acataatatt tatcgtagct ttaacaactc gaaattagta
    13501 taggccgcta atttcaatca tacaacaaaa ttttgttagc agcctaagta taagtatatc
    13561 gatatagatc agtgtgattc gctatgagcg acttcggcat tgccatgatt ctggcagcca
    13621 acttcttcac aacgttgcat catgtaaccc agcgcgacca gcactactgt tgataacatc
    13681 acgctggtgg tggtgagcaa tggcgtgctg atactgatca gccaggaaac taccagactt
    13741 gcgaggaagc acagacccag ttgcagagtg ttctgcaacg ctgcggcgcg accggttgca
    13801 tgtgggaagg gacgcagcgc ctgggcgaca acaatagggt agatcgcgcc attggcaatc
    13861 gccatcacac agaatgggat caggatttcg accagcgaca catggctaat gaagcccgca
    13921 gcccaggtcg caatgacgct gacagcaaac agcaccagca accacggtaa taactgcttg
    13981 ccttgccatt tctgcagtgc ggcgcgacag ccataaccac caatcagaaa cgcgatagtt
    14041 tgcgggacat aacttaaacc aataactgcc gggctgtagc ccatttcact aaggatgaac
    14101 ggtgaaccgg tcagccatgc aaaaaaactg gctgaacagg ctgcatatat cagcacgttg
    14161 ccgcgatagg ttttagaacg tagcaggtcg gtaaaggtca gaccatcctg actattgtta
    14221 cgggccttcg tcgtgggttt gagccagaaa ataggcagaa tcagcaccac ggtaatggca
    14281 aacagggtgg cgaaaatcgc ctgccaggaa aaatggacca gcagccagct tcctaacagg
    14341 ggagccagtg ccggagacag acccaccagc ggcatgatgg tcgcaaaaat acggttgact
    14401 ttctgtgaag gataataatc tgtcactaat gcttgccaga taaccgccgc ggcgcagacc
    14461 cccacagcct gtacaaaacg caataccagc agcgtagcgg cgttttctac ccacagcatc
    14521 cccagactac ctaacgcaaa aattgtcagg ccgattaata acaccggttt acgaccataa
    14581 cggtcggaga gtggccccca cagaagctgg gctgcggcaa aaccggcaag gaacagacta
    14641 aggctggcac tgacagcaga cgcaggcgtt tgcaggtcgg cctgtatggc ggcgaaagca
    14701 ggcagataca tatcggttgc cagaaaaccg agtacgctca aacccgccag ccagactaaa
    14761 aatcttttcc caggttgcat gtatattctc taaatctctc aattaaacac cgccgcaaag
    14821 tgtagggagt gaaattagtg ttgtgaaacg gtaatatttg atgactggtt tcaaaaattt
    14881 tgcaggcaga ctatgtggtc agaatattca ctcgaagttg ttgatgcggt agcgcgtaat
    14941 ggtagtttta gcgctgcggc acaggagctg catcgcgttc cttctgcggt cagctatacc
    15001 gtgcgtcagc tggaagagtg gctggcggta ccgctctttg aacgacggca ccgtgatgtg
    15061 gagctgaccg ctgctggagc gtggtttctc aaagaagggc gctctgttgt caaaaaaatg
    15121 cagatcaccc gccagcaatg tcagcagata gcgaacggct ggcgcggtca gttagctatc
    15181 gcagtggata atattgtcag gccagaacgt acacggcaga tgatcgttga tttttatcgc
    15241 cattttgacg acgtcgaact tctggtcttt caggaagtgt ttaacggtgt ctgggatgcg
    15301 ctttccgatg ggcgcgtgga actggcgatt ggcgcaacac gcgcgattcc ggtaggcggt
    15361 cgttatgcct tccgggatat ggggatgcta agctggagtt gcgttgttgc cagccaccac
    15421 ccgctggcgt tgatggatgg cccgttcagc gatgatacgt tgcgcaactg gccgtcgttg
    15481 gtgcgcgaag acacctcgcg aacgctacct aaacgtatta cctggttgct ggataaccaa
    15541 aaaagagttg tcgtgcctga ctgggaatca tcggcaacct gtatctcggc aggattatgc
    15601 atagggatgg tgccaacaca tttcgccaaa ccgtggctta atgaggggaa gtgggtggca
    15661 ttagaactgg aaaacccctt tccggattcg gcatgttgtc tgacatggca gcaaaatgat
    15721 atgtcgcctg cgttaacctg gctgcttgag tatctcggcg atagtgagac gctgaataag
    15781 gagtggctgc gggagccgga agagactccc gcaacgggtg attaacgacg atagtcgcgg
    15841 aacgggccgt cagccacgga gcggcgttca atcaagcgcg gatgcacttc aatggactgc
    15901 ggttcttcac gtttgttgac gatacgatcc aacagcatgt tgaacgctgt ttcacccagc
    15961 gaatcttttg gctggtggat cgtggtcagc gccggcgtaa aatagcgggc gttgcgcacg
    16021 ttatcataac cgatcagcga aacatcctgc gggacgcgca gacccatttc atcagcagca
    16081 caaagtgcgc ccattgccat gatatcgcca ccacagaaga cggcagtagg gcgatgcggc
    16141 tgcgacagta tttgctgcat ggcgcgataa ccagattcgg gttcaaagtc accctgaaca
    16201 atccagcttt ccggcacctt gatcatcgct tcttccatcg ccttcataaa accggcaagg
    16261 cggcctgcgc cggtgttacg ttccagcggg ccggggatga cgccgatttc gcggtgaccg
    16321 cgttcaatca gataacgccc ggccatgtag ccgccttcga acgcgttatc aatgaccgca
    16381 tcggtgaagt cagcttttgc ttcaccccag tccatcacca ccattgggat atggcgatac
    16441 tcttccagca tcgccagcaa cggctctggg tactcagaac acatcaccag cagaccatcg
    16501 acgcgttttt gcgccatcat cgacagataa gcccgctgtt tctcaagatt gttccacgca
    16561 ttacccagaa tcagggtgta acctttctgg aagcaatttt tttcaaccgc ttcaatgatc
    16621 tcggcaaaat aggccgcttc gctgctggtc gccagcaaac cgatagactt ggtgtggtta
    16681 accttcaggc tacgcgccac cgcgctaggg gagtagtgta attctttaat cgctgcccac
    16741 acggcgttgc gcgtttcttc agcgacgaaa cgtgttttgt tgattacgtg tgacacagtt
    16801 gtagtggaaa cgtttgctcg tttcgctaca tcttttattg ttgccattcc atttcactcc
    16861 agaccctaac ttcagctcct gcaaaatcgc aaggtaaacg tttgccttta cacaccttac
    16921 gcacaacaaa agtgttgcgg tacgccggaa aaacgacacg gagcgtcagg aaggggtcaa
    16981 tggccagtac gcaaatagtg taagcgtgga attttggccg atcttgacga aaaggggaag
    17041 tggtaaaaac acttatcatt ataaatgacg ggagattttt tcatcacagt gtgtaaaaat
    17101 gcgtaatatc actatttgtg tggtaataaa aaaggagaaa acttgatgag caccgacctt
    17161 aaattttcac tggtaacaac gattatcgtc ctcggtttga tcgtggccgt gggtttgact
    17221 gccgcgctgc actgatttcc tctcgaggga gcgcgttctc cctcgcatct ccttcctgtt
    17281 tgttagcaaa atggcaaaag tgttttctct ttttgttatt ttcatttttt tgtgattgct
    17341 gtcatgcttt cgccacttta atgaatatcg cgtaatgacg cgaagaggtg gagataaggc
    17401 atgaaaatta actatccgtt gctggcgctg gcgattggcg cgtttggtat cgggacaacg
    17461 gagttctctc caatgggttt gttgcccgtc attgcgcgcg gtgtggatgt ctcgattccc
    17521 gctgccggaa tgttaatcag tgcctatgca gttggcgtaa tggttggcgc gccgctgatg
    17581 acgcttctac tttctcatcg tgcccgccgc agtgcgttga ttttcctgat ggcaattttc
    17641 acgctcggca acgtactttc cgccatcgcg ccggattata tgaccctgat gctttcacgc
    17701 attttgacca gcctgaatca cggagcattt tttggtttgg gttcagtcgt ggccgcaagc
    17761 gtggtgccaa aacataaaca ggccagcgca gttgccacta tgtttatggg gttaaccctg
    17821 gcaaatatcg gtggcgtgcc ggcggcgacc tggttgggtg aaaccatcgg ctggcggatg
    17881 tcatttctgg caacggcggg gctgggagtg atttcaatgg taagtctgtt cttctcatta
    17941 cctaaaggtg gtgcaggggc acgacctgaa gtgaaaaaag agctggcggt attaatgcgt
    18001 ccgcaggtgc tgtctgcatt gctgacgacg gtactgggag ctggtgcaat gtttactctc
    18061 tacacctata tctctccggt actacaaagt attacccacg caacaccagt gttcgtcacg
    18121 gcaatgctgg tgctgattgg tgtcggattc tctatcggta actatctcgg cggcaaactg
    18181 gcagatcgtt cagttaacgg cacgttgaaa ggctttttgt tgttgttgat ggtgattatg
    18241 ctggcaatcc cgttcctggc ccgcaatgag ttcggcgcag ctattagcat ggtggtgtgg
    18301 ggcgcagcaa cctttgcggt cgtaccgccg ttacagatgc gcgtgatgcg tgtcgccagt
    18361 gaagcgccag gtctgtcttc atcagtcaat attggtgcct ttaatcttgg aaatgcgctg
    18421 ggagcagctg ctggtggtgc ggtaatttcc gctgggctgg gatacagctt tgtgccggtg
    18481 atgggagcga ttgtcgcggg actggcatta ttgctggtgt ttatgtcagc cagaaaacaa
    18541 cctgaaacag tttgcgttgc taacagctaa ttaaacaaaa acgcagaagg ataaccttct
    18601 gcgttacagt tgctgacaac gtgcgattta tcttgccgaa tgcgctgtta acgcctcatc
    18661 cgcgttggca tggtaagcga agcgcatcag gcaatgctgc atttgccatc agttattatg
    18721 cagcgagatt tttcgctacg aattcccagt tcaccagcgc ccagaagtgc tccagatagc
    18781 caggacgtgc attgcgatag tcgatgtaat aagcgtgttc ccagacatca acggtcagca
    18841 gcggagtcgc atcggtggtc agcggagtac ccgcgttaga ggttgaaacg atagccagtt
    18901 tgccatcgct gtttttcacc agccaggtcc agccagaacc aaagtttttg atcgctgcat
    18961 cagtaaactg cgctttgaaa tcggcaaagc taccaaaaga tgcggcgata gcttcagcga
    19021 cttttccagt cggttcgcca ccggcgttcg gtgccaggca gttccagtag aaagtatggt
    19081 tccagacctg agctgcgttg ttgaatacgc caccttcaga gctgcgaata atctcttcca
    19141 gtgatttacc ttcaaacgcg gtacctttaa tcaggttgtt caggttagtg acataagtct
    19201 gatgatgctt gccgtagtga tactcgatag tttccgcaga aatgtgcggt gccagagcat
    19261 ctttagcata tggtagtgca ggtaattcga atgacattgc tactctcctt tattattaat
    19321 ttgcatacgt acaatagcct tattgtgcgt atggataggg tagcaaatta aaaggtgaga
    19381 caaaagatga acttaccctg ttgccgtaac aacagggtaa agttttagcg aagtgttttt
    19441 ggggtcatta cccgacgagc gccaacatag tggcgctgcc agtagtcttc actgagagaa
    19501 gtgatttgaa tttcctgacc tgtgcgcggt gactgaataa atttgccgtt gccgacatac
    19561 acgccgacat gatcggctgt gccgcgtccc tgagtacgga aaaagaccag gtcgccgttt
    19621 ttcagttcac tacgttcgat tggcgctgca tcacgcaggt gatacatttc attcgccgta
    19681 cgcggaatac gaattttcac caaatcttta taagcgtaat aaaccaggcc gctgcaatca
    19741 aaaccggtac gcggtgagct gccaccccaa cgatatggct taccaatttg ctgcatcagt
    19801 ttattcattg ccactttcgt agctttttgc accttcgctt tgtgcgcgtc ggcaagagtt
    19861 cctgacgcgg catttttgac tttcacacaa tgcgatttat agcccttacg tttggtacac
    19921 ttttcggtaa agctggctga ggccgtttta ttgacggcgt gtttgctgcg agttttggcg
    19981 tttttagaag cggtaatgga agatttttta acggaagaag cggttttgct tttggtcgta
    20041 cttttactgg cggctttttt cgctgttttc tggctggctt tttgggtctt tttggtggtt
    20101 gccgtgcttt tctttttatc tgcttttttg gtgatatgag tggtagcaga actctccctg
    20161 gcttgctttg aagcatgggc cataggcgtt aaaggcaggg tggtaaaaag taaagcacag
    20221 agcgtgatcg aaatacggtt tatccgcgcc actgagcgtc cctctgttaa ggcaggtcta
    20281 tcatgcctgc gtctgattta caaaacttga tgattctaat gcatagaaga agagaaagtt
    20341 aatagctttt attgttaaaa aatacgtttc gttggcgagc gcaaaaaaat gcagcagtga
    20401 aagctaaaaa tggttataaa tacgccagtt tgccgagcat ctgggaagaa acgaatattt
    20461 aaaatgcaac aaatagagca aagggataaa atagcaaaag cgtgatgaaa atgttatttt
    20521 ccgatgccgc actgacccgc tacaatgtca gattactgcc gtaaagaagt ttgaggaagc
    20581 aagacaatga gcaccactat cgaaaaaatc caacgccaga ttgctgaaaa cccgatcctg
    20641 ctgtacatga aaggttcacc gaaactgccg agctgcggtt tctctgccca ggcagtccag
    20701 gcgcttgccg catgtggcga acgttttgcc tatgttgata ttctgcagaa cccggacatt
    20761 cgtgcggaac tgcctaaata tgctaactgg ccgaccttcc cgcaactgtg ggttgacggc
    20821 gagctggtag gcggttgtga tatcgtgatc gaaatgtatc agcgtggcga actgcagcag
    20881 ctgatcaaag aaactgccgc taaatacaag tctgaagagc cggacgcgga ataatccgtc
    20941 ctgcaatact gacggatgcg aacgggcatc cgtcagtcct ttctctatcc ccaatccagc
    21001 ccttgtggcg aacttgaaaa tcccgcctca cgcagcagcg taaacatcgg cgtttgccgg
    21061 accggtagat cattcacttc tgttagcgta aagcgcagcc gtggttcgcg acgcagtgcg
    21121 gtagtcagcg cgtggaaaac ctctggggcg agtaattcct ctttttcctg ccagaccagc
    21181 atttttttgc caccttgcgc cagatagagt aacaatttgc cgccagaaac gaccaccagc
    21241 gcacccgccc gacgcgttgg aaccagcgtt gccggatgtg caggccaggg cagaagattt
    21301 ccccacacat tagccggatc gttggcagag agcgccactg gtgtatagtg gcgcgtttgc
    21361 gtggcttgtg tcgccagatc gcgcaatcgg tcaatagtca gacgttcagc gaattgcgcg
    21421 ccacccagac cttctacaaa acgacctcgc ataattcgcc cggagtcttc catacttcga
    21481 caaagcgttt gcatcgatgg aaacccgcca gggatatttt cggctatcac cgcctgacga
    21541 ctgatgatgc cgtagcggtc gagcatattt tccgccagcg ccagcatcct ttcggtatcg
    21601 tttagtggct ccacctgcaa taacgaccag cgtccagcca gatttggtgt gttgtaagat
    21661 accagcggcg agacgggttg cgcatagaca ggacgtccac gacgagcccg gtgactgcgg
    21721 cgagttgagg tgcgtgcgtt ggaactgctg cgggtgaggg cgcgtaacgg tgcccaaatg
    21781 tcgctggtga tgacgccttg ccagaccagc gcccataacg cctcttgcag ggcagagaga
    21841 tcaaccgatt cgccgatttt gtcgcgtatc cgttggctga tttgttgtgc aaaccaggct
    21901 cctccgtcag ccagaacagc gattatcgct tgctgcagcg ccgaacgatt cgcctgatcc
    21961 gcttcggcgg gagtgaacga ttctgcggca tattcctgta gatgcagtgc caccaggccg
    22021 tcatcttcac ccagcttttt ttgtcccgac cagataaccg ctccggttgc cagtaattca
    22081 tcgagcattt ctggcgaata gtcgcgtacg cgggcaggca ggatctggct ttcccagagt
    22141 gaggcgggta aaccgactcc ggcaagctgt tcgatcaccc gcatcacgcc atctacgccc
    22201 tcataaacgc ctggcgatgt tgaggcaaag agcgccgggc taccatcggt ggcgggtaat
    22261 acgccctgac gttccagcag caatcgcgca taggtcgtgg ctgcaacggg acgcgtcgct
    22321 tctctggcgg cttgcagcga gcgcaaacgc agacgacgaa atacttcatc gctcacccag
    22381 atgtcttgtt gcagattcat caccagaccc tgttcacgca gttgctgaag ctgctcttcg
    22441 acaatggcaa taccgagact aaactcatga gccagttgtt cagccgtgac cagagcatga
    22501 gcccggagat agcgcagaaa gaggtcgcga agcgggtaac tgactctatg taaataaatc
    22561 tctggcaatg actctggtag tcgtacgccg agggcatcac gcagcctggc ggcatcatcc
    22621 atacaggcaa gacgctcctg tccgctaatc atcgctggga agattcgttt tactgcaaga
    22681 agattttcca gatacgacgc aacctcttca ctgctgcctg tatgccgttg cgccaggtct
    22741 tcaacggtca ttggccccag ttcgcgcagc aggtcgaaca atccttcttc acctttcgct
    22801 cttctgccag gagccagtcg ttgcaactct tcttccacct ggcgaatgac ctgcgggtcg
    22861 agtaattccc ccggatcgac ctgtccgagt agattgcgca gtaactcgct gtccagcgac
    22921 agtacggatg cccggcgctc tgccagcggg gcgtcgctct ggtacataaa ttccgcgaca
    22981 tagccgaaca ataaacttgt ggcgaaaggc gagggcgtag tggtcgttac atcggatatt
    23041 tgaatttcgc caccgttcag gcgacgcatc aaacgttcca gtgcgggaag atcataaaca
    23101 tcttgcagac attcgcgtag ggtttcgaga atgaccggaa aatccggata tccctgagcg
    23161 atttccagca actgactggc gcgcagccgt tgttgccaaa gcggggtgcg atggcccgga
    23221 gtgcgccccg gcattaataa tgcccgcgcg gtgcattcgc gaaaacgggc ggcgaaaagt
    23281 gccgagctgc ctaccgcctc gcggacaatt tgcagcaact tttctggttc aaacaaaaaa
    23341 atcgcggcgt cgggcaattt accatcagtg tcaggaatgc gcgcaacaat gccgtcatca
    23401 ctggcgacca cagacgcgtc agcgccccat agggcatgta ttcgcccggc aatcgccagc
    23461 gcccagggtt catgcacccg tcttccatag ggagagtgca aaataatacg ccagtcgcca
    23521 atttcatcac ggcaccgttc gaggaccaga tggcgactcc ccggaacgat gcccgtcgcg
    23581 ttgcgttgct cgtcaatcaa cccctgaata ttggcgttcg tattttcttc tgccagccac
    23641 gggggaatag tcccggaaaa gaacgcgcca tccgccagta aatgaagaaa atcgccgatc
    23701 atctcgccta attcagccgg acgtccgtta ccttcaccac gccagaaggg gagccgggca
    23761 gaacgacccg gagcaggagt cacaatcacc tgatcgcggg tgatttgctg gatccgccat
    23821 gaggtagcgc cgagagtgat aatgtcgctc acccgcgact catataccat ctcctcatcc
    23881 agttcaccca cccgccgcga accggcctgt tcttcacctt cgggtaataa cacgctatac
    23941 atgccacgat ccggaatggt gccgccgctg gtaaccgcca acaattgagc gccaggtcgg
    24001 gcggtcaata tcccggtctc cctgttccag accagtttgg ggcgaaaagc agaaaaatcg
    24061 ccagagggat agcgcccgga aagcatatcc agcgtggcgt caaaaacacg tcttggcaga
    24121 tctttccacg gtgcggcacg gcgtacgcgg gagtaccatt cgtctacctg taatgcatcc
    24181 atcgccgcgg cggcaacggt ttgctgcgca aggacgtcga gtggattatg cggcggtgtc
    24241 aggttttcca gcctgcctgc gaacatacac tctacaatga ctgcggaatc gactaagtca
    24301 cgccgggtac gagggaaaaa cagcccttta gatacaccgc caacctgatg tccggcgcga
    24361 ccaatgcgtt gtaacccact ggcaacagaa agcggcgttg ccacctgaat caccagatcc
    24421 accgcgccca tatcaatccc cagttcgaga ctggaggttg cgaccacgca gcgtaattca
    24481 cccgatttca gcgcctgttc ggtgattgct cgttgttctt tagagacgga gccgtggtgt
    24541 gagcgggcaa taaaaacgtc gctactttgt acatggttag aggttgcgcc ggaggtcgac
    24601 tcgaaatggg ccgtatcaac ggcgatagac ggggaacgct gtaagcgtgc ggcgtaaagc
    24661 tcatttaatc gtgccgtcag tttttccgcc agcccacgtg aattagtaaa gacaatggtc
    24721 gagcgatggc gcaacacttc atcaaggata cccgtttcaa tatatggcca gatggagcct
    24781 tcccggccgg catggctgtc ttcgccggtg ccgctggcga ccgatgagac atcatccata
    24841 ttggcgaccg gtacgacaat tcgtatctgc ggatggcgca ttgcgggcgg gttgactacc
    24901 gtaaccgggc gatcgccacc aagaaatgct gccacatcgc tggctgagcg cacagtggca
    24961 gaaaggccaa ttcgctgtgc tgaggtgtgg agcagcgcat cgagccgctc cagacttaac
    25021 gccagatgcg caccacgttt actgcccgct accgcgtgga cttcatcaat aattaccgtt
    25081 tcgacgccgc gtagcgtttc gcgcgcgcgg gaggtcagca tcagatagag tgattcgggt
    25141 gtggtgatca gaatatccgg cggattacgg gtgagtttgc tgcattcctg tgcaggcgta
    25201 tcgccagtac ggatccctac gcgaagattg acttccgttt cgccgcgccg ccgccgttca
    25261 tcggcaatac ccttcaacgg gagctgcaag ttgcgctgaa cgtcggtgcc cagagctttt
    25321 atcggtgaaa tatagaggat gcgtgaggtt ttacgcttat gcgcctcgcg ggtatcttcg
    25381 ccgccttcgc ggaagagccg atcgagggcg tagagaaatg ctgccagcgt tttcccggag
    25441 ccggtcggtg caatcaccag cgcatgttcg cttcgcgccg ccacatgcca ggtttgcgac
    25501 tggacagcgg tcggctgttt aaaggcgcga agaaaccagt cgcgggtcgc cggtgaaaac
    25561 acgtccggca ggagcgatga agggtctgga ttatctgcca ttaacgctat tccccgtgaa
    25621 tatcatgctt gatgcgtgga atagcctcac tatagatgcc tgaatcattg catgtcatca
    25681 ggcatcgact cgattacacc tcttcggcgg caggtagcgg ccagcctccc agacgtttcc
    25741 agcggttgac gatttcacaa aacagcacag cagtgcgttc ggtgtcgtac agcgcggagt
    25801 gcgcctgggt gctgtcgaag tccatgccag cggtctggca agcctttgac aatacggttt
    25861 gtccgagtgc cagcccggcc agtgcagcag tgtcaaaagt ggcgaaaggg tggaacgggt
    25921 tacgtttcag tgaggcgcgt tctgcggcgg ccatcataaa gctgtgatca aaattggcat
    25981 tgtgcgccac cataatggcg cggttacagc cgctcgcttt aataccttta cgtacaactt
    26041 taaaaatttc gtgcagcgcc tcgtattcgc tgaccgcgcc gcgatcggga tcgttcgggt
    26101 caatgccgtt gaaggcgagg gcctctggct gcaaatttgc gccgacaaat ggttcgacgt
    26161 ggaaatgtaa tgtggtgtcc ggcatcagcc agccttgttc atccattttc agggtgatgg
    26221 cggcaatctc aagcagcgca tcggttttgg cgttaaatcc ggctgtttca acatcgatca
    26281 caacaggata aaaaccacga aaacggtcgc acagaccggt aagttgagcg ttatcggaca
    26341 tctgattctc ttaatacgga taaattgcag cgcgcattat gacaaatatt acttgtcgat
    26401 gcagtaaaga tgcgggcgcg atgagttcac gcccggcagg agattagttg cccagaccgc
    26461 gaccggcgtc tttctcttcg attaactcaa ttttgtaacc gtccggatct tccacaaacg
    26521 cgataaccgt agtaccgcct tttaccggac ccgcttcacg ggtcacgtta cccccgtttt
    26581 gacggatttt ttcgcacgct tcagcggcgt tatctacgct aagcgcgatg tgaccataag
    26641 cagtgccgag ttcgtattta tccacgcccc agttgtaggt cagttcaatc accgcttctt
    26701 cggtttccgg gccgtagcca acaaacgcca gtgagtattt gtattccggg ttttcgctgg
    26761 tacgcagcag tttcatgccc agcactttgg tataaaaatc gatggagcgt tgcagatcgc
    26821 caacgcgcag catggtatga agaagacgca tctttttatc ctcaaaatgg ctaacagatt
    26881 caaaatgttg ttttagtata gcggcggctt tacgccgctc gcaatgttgg attacaacgt
    26941 cgggtaatcg gtatagcctt ccgcgccgcc accgtagaaa ctttcggcac gctgtgggtt
    27001 aagctcagct ttgcgctgca agcgggcgac cagatccggg ttcgcaatcc agtcacgacc
    27061 aaatgccacc gcatcaatta accctttgcc gatcagcgtt tcagcttttt ctactgtgta
    27121 tgcacctgcg ccgataatcg gaccgtggaa acgggcgcgt actttttcgc ggaacgcatc
    27181 agtatacggt tcaccccccg cccaatctgg ttctgacata tgcagataag caatgccgcg
    27241 tttacccagt tgttcaatca gatacagtgc atcggcttct tcattcgcgc cgttatccgt
    27301 gttctggaaa gtaccgattg gcgaaacgcg aatgccaatg cgatcggcac cccattcttc
    27361 aatcccggca tcgaccactt ccagtaccag acgtgcgcga ttttccacgc tgccgccgta
    27421 ctgatcggta cgatggtttg aagaaggaga aaggaactga tgcagcaaat aaccgtgagc
    27481 agagtggagc tctaccagat caaaaccggc ttcacgcgcg ttagcaatgg cctgacggaa
    27541 atcattgacg atacctggaa tctcttccag ttcaagcgca cgcggcatgg atgtttcaac
    27601 acggatcgcc tgaccatttt catcgcgcag agaagtacgt gttcccgcgc taagtgctga
    27661 aggcgctacc ggtgcctgac cgccaggttg caggctggcg tgagaaatgc gtccggtgtg
    27721 ccacagctgc acggccatat gaccattttc agcatgaacg ccagcggtga tttttttcca
    27781 tgcggcaatt tgctccggac tatggatgcc aggcgcacct gcatatcctt ttgcctgggc
    27841 agaaatttgc gtggcttcac taataatcaa accggcactg gcacgttggc gatagtattc
    27901 cgccatcaac ggggtaggaa tgtcacccgg ttcaatactg cgcagacgcg tcagcggtgc
    27961 cataaaaata cggtttgccg ccgtgatcgc gcccactttc agtggggaat acagtttttc
    28021 agatgacata aaggctcccg gtcgtctggt gataaaaaat gcctgctaaa cggcaggcgt
    28081 cgcaataatg ttttttacat gggccagcgc gttttccagt ggctcgaaac tgcgcgaaat
    28141 tttggcctgc agattcgcac ccagccatag tgcgtaaagc acttgtgcct gttgtagcgg
    28201 ttcgccacaa aaggttaaac aatggttctc acggccattt tccagcgcct gagagagcag
    28261 ggcgatcacg ccgcgagcac ctttatccat cgcgctgcgc atatcttctg acagatcgca
    28321 cacttcggca gagagtttta ctgtcaggca accactgatg gttccatgtt ggcaaaactg
    28381 gttcagtgtt tgctggtaat aagccagtat gcggtcgcgg tagttacctt cgccggattg
    28441 cagcaactca gtcagtcgct ggtgatatgc ggcgtaatga cgctcaagca tggcaacgcc
    28501 aaacgcttct ttagagcgaa agtagtgata gaaggaccct ttcggcactt cagcggtttt
    28561 tagtaattcg cttagcccca tcccggtgaa tccacgttgc aggcaaagtt gctcgcccgt
    28621 cgccaggaga tgttcgcgag tatcatgttc ggtgtgtttg ttcatgcgtt ggagtgtagt
    28681 agaccagtcg gtctagtgca agcaaagttg ctactttgga cattaacagc ttcaataatg
    28741 gtaataatct tatctgagga gggcgaagtg gcggagcaat tagagttctt tcccgtccag
    28801 agcccgtgcc ggggaatttg ccagtctgac gaacgcgggt tttgtcgcgg ctgttttcgt
    28861 agtcgtgatg agcgttttaa ctggaataaa atgagcgatg gcgaaaaaca agaggtgctg
    28921 cgcctttgtc ggcagcgatt aatgcgcaaa ttacgcgcaa ataagccagc gtcatcagac
    28981 gaaccggagc aaccatcact cttttgaacc taatcttgcg tatactcctc tcatatctga
    29041 taagaggaag tgattatggt tcagcgtatt actattgcgc cgcaaggccc ggagttttcc
    29101 cgttttgtga tgggctactg gcgattgatg gactggaata tgtccgcccg ccagctggtc
    29161 agttttattg aagagcatct ggatctcggc gtgaccaccg tggaccatgc tgatatttat
    29221 ggtggctatc agtgcgaagc ggcgtttggc gaggcactga aactggcacc tcacctgcgt
    29281 gaacggatgg aaatcgtcag taaatgcggt atcgcgacga ccgcgcgtga agaaaacgtc
    29341 attggtcatt acatcactga ccgcgatcac atcattaaga gcgccgaaca gtcgctaatt
    29401 aatctcgcga ccgatcatct ggatctgctg ttaatccacc gaccagaccc gttaatggat
    29461 gccgatgaag tggcggacgc gttcaaacat ctgcatcaga gcggcaaagt gcgtcatttt
    29521 ggcgtatcga actttacgcc tgcgcaattt gccctgttgc aatcacgtct gccgtttacc
    29581 cttgccacta atcaggtgga aatatccccg gtgcatcagc cgttactgct ggatggcacg
    29641 ctcgaccaac tacaacaact gcgtgttcgt ccgatggcgt ggtcctgcct tggtggtggt
    29701 cgtctgttta atgatgatta tttccagcag ctgcgtgatg aactggctgt ggtggcagag
    29761 gagttaaacg cgggctcgat tgaacaggtg gtttacgcct gggtattacg tttaccatcg
    29821 cagccgctgc caattatcgg ttcaggtaaa attgagcgcg tacgggcagc tgtcgaagca
    29881 gaaacactga aaatgacccg tcaacaatgg tttcgtatcc gtaaagcggc actggggtac
    29941 gacgtaccgt aagcactttt aggaatagcc gccgttcaaa aatgtgtcac tggtttacac
    30001 ttattcagga atgcacaatg aacggaggtc ctatgaaacg ttttagtctg gctattctgg
    30061 cgctggttgt tgcaaccggc gcacaagctg ccagtgaaaa agtcgagatg aacctcgtca
    30121 cgtcgcaagg ggtagggcag tcaattggta gcgtcaccat tactgaaacc gataaaggtc
    30181 tggagttttc gcccgatctg aaagcattac cccccggtga acatggcttc catattcatg
    30241 ccaaaggaag ctgccagcca gccaccaaag atggcaaagc cagcgccgcg gaatccgcag
    30301 gcgggcatct tgatccacaa aataccggta aacatgaagg gccagaaggt gccgggcatt
    30361 taggcgatct gcctgcactg gtcgtcaata atgacggcaa agctaccgat gccgtcatcg
    30421 cgcctcgtct gaaatcactg gatgaaatca aagacaaagc gctgatggtc cacgttggcg
    30481 gcgataatat gtccgatcaa cctaaaccgc tgggcggtgg cggtgaacgc tatgcctgtg
    30541 gtgtaattaa gtaattaaga ggccagcgta ccttgcggtg gtgcttgctc aagttgcgaa
    30601 agcgagcagt acagccgcca gacaattgcc gccagctcac gggcggcagg ttgatgatga
    30661 cgggcaaggc tgtcgcaaat ccgctgtaat tcttcaagtg tggccgccag tgatttttgc
    30721 tgaacgccac gctcactcat cactccccgc aacagtgaaa tacagttatc ccgtactcgc
    30781 gataacggat cggaacgcga ttcccagtcg cgcaattgcc agacaacatg agaacagttc
    30841 agcagcacta caccccagcg taataaccaa cggcgagcca gcgcatcctg gctgttactc
    30901 aactgactga cgtgatgata agtgagcgat tcaaattcgc tttcactcag tgttggatgg
    30961 cggcttagct gatcgacaaa atcccggcgc agcgcgcgaa tatggcggcg gcttttacga
    31021 gcatccgatc ctggacgcag aatggcgaac gctagccaag ccagcgcaac gccaacgatt
    31081 tttgccagat tatcgttaag aaaatcagca aaatcataca ccgggggatt agtgacagcg
    31141 ataaacgaac ccataaaaac aatcagttgc ccccacagtg cggcaaattt gggcatctgc
    31201 aatttaagaa gctgcattgt cgccagcagt ggaaagagaa acagtaaaaa ttgccacaga
    31261 tcgctaatct ggaccatcag gccaaatttg accacaaagc tgaatagcga aagtaacacc
    31321 agcgtgcgca tcagcagtga caacgactta aacggtgctg cgacggcgga gtagagtacg
    31381 cagctaattg ctgccagcgt taatgcattg gcaccggcat cccattgcga tgcaatactc
    31441 catgcgccaa tcatcatcaa cgtacaaaat gtacgcagcc cgctccacat agcttcggcg
    31501 ttgtcggtat gacgagccag agcggatgtc cgtgcgagtc tggcatgatc atctacaccg
    31561 ctttgcagac gatgtaattc ctgactactt tgcagataaa ggcggcaaaa ataacgtagt
    31621 cgctgccaga aggcgacgtg ccggtagtcg gcgacgttgg tcgggcgtag cggggcgata
    31681 atacgtgcga cggtgtaagc atctgtttac gaactggcga gcgccgtcag caattgttcg
    31741 agaatttctc gcgtggcacc tggcggtgag ggccagttga gcaacatacg tcgcaggctg
    31801 gagatgacac tggtcatacg taattgctgg tggagtagcg cattaaggcg cgcgttttgc
    31861 tggcgaaaac gatagtggct ccagaaagcc tggatacgca gcaaattcat ggtcagtatc
    31921 tgcccaatca ccccctcatg tgctgcacga atggcatcgt ttgtttcagg ctgccagagt
    31981 aaactggcat gttccagtaa tcgggcgtgc atgtttttca atgcggttaa aagggctgta
    32041 gcatcggaac tgctaggcag gatcatcatc atcatgccgc cgcacaaaat accgactatc
    32101 acctcgcaaa cgcgcgcctg agcgatatcc cacagctggc tggcctcagt aatattaacc
    32161 atcggaaagg cgatgatggc agccgtgtag cccgccagtt gaaatgcata cgcgacgtta
    32221 ttcgtgaagt gcgcacaggc ccaggtacaa aagccaagcc acgccgacat gctcaataga
    32281 aaaaaccacg gctcattgag cgtatgcccg gcaagaagca gtgccgcaat ggctcccagt
    32341 aaactgccag cgatgcgtcc gaggcttttg ctgatgacac cgccaacggt gggaaagcta
    32401 accactgcag ccgaggtcat cgcccaatag ggttcatcca gattcaaata ataggcaacc
    32461 gtcagcgcca gacacatggc aatggtattg cgtaacgcat aacgccattg cgccagcgtg
    32521 gccttgaacc agggcaaatt gcgcaaggac catgacgatg cgttcattat tgacctacgg
    32581 caatgctaca ggtggtgccc gacaccagcg tgacatcgcc tggaacttta tccagcgcaa
    32641 agcggacggg aacgcgttgg gcgagacgaa cccaggggac gttaggtttc acatccggaa
    32701 taaggcttga gtcactttca acgctttgat cataaatcgc ccgaccgata ctggaaacgt
    32761 gaccccgtaa cgttttgtta tcactataga gggtaatttg tgctggtgca ccttcgcgga
    32821 tatggcgcaa cttggtttct tcaaaataac caatgacgta aaacgagtgg ctgtcgacaa
    32881 gggcaaacag cggtttcccg gtgtcagcgt aatcgccgat gcgggtagtg aggttagtca
    32941 cccaaccact caccggggcg cgaatttccg tttgcgccag ttgccattgt gtctgtttca
    33001 gcgtggcctg tgcggcatta acactggcct gcatcgcttt aacattgagg ttagcggtat
    33061 cgagttcttc ggcagaaata aaattttgtg agagatgacg acgacgattc gcttcgttat
    33121 tggcttttgc gaggtcagat tgcgctttcg ccagctgagc ctgtgcgttc agctcggcga
    33181 tctgaaaggg cgttttatcg atcgtgagca gaagatcccc cgcgttaacc agttggttgt
    33241 cttttatatt cagctcaaca atacgtcccg acacctgtgg ggtgatagaa accttttctg
    33301 cgcgtatttt cccgtcgcgt gtccacggag attgcatgta ataattccac agccaccatc
    33361 cggcaagaac cgcaactatc gctacaatga ttgttgagaa atacttaatt gttttaattg
    33421 acatagttac cacgcaatca gtattgccag agcaaggcaa acgcaaatcg caaacagcga
    33481 taaatccatt aacaagggat gccagatgtc accggcgtag atccagccac gaagcaggcg
    33541 gtgtacgaca agccagatga cgaatccaaa cgcgaaggct ttgaaaaacg gaggaaaata
    33601 gacggacgca ccgaacacca gatcttgtaa gggcaatcct gttgcattga gcataaactt
    33661 cacaaagtag attcctttac gaccgaatat attgcgttag attaataaat attctttaag
    33721 tgcgaaaaat ttacgcgcaa tttctgaaaa atatgtctga cggtaaccaa atgcagcaat
    33781 acatttgttt tagcaataca attgctgcac actattctaa aagccgcata atatcttagc
    33841 aagctaatta taaggaggtg aaattggaat cgccactagg ttctgatctg gcacggttgg
    33901 tgcgcatatg gcgtgctctg atagaccatc gcctgaaacc gctggagtta acacaaaccc
    33961 attgggttac gttacacaat atccatcagt tacctccaga ccagtcgcaa attcaactgg
    34021 caaaagcgat tggcatcgag cagccatcac tggtccgtac tctggaccaa ctggaagaaa
    34081 aagggttaat ttcgcgtcaa acttgtgcca gcgatcgtcg ggctaaacgt attaaactga
    34141 cggaaaaggc agagccactg atcagcgaaa tggaagctgt tattaacaaa acccgcgcgg
    34201 aaatattaca tggcatctcc gcagaggaac tggagcaact gattacgctc atcgcaaaac
    34261 ttgagcataa tatcattgag ttacaggcca aagggtgaaa tgaagggggc gtgtggtcag
    34321 taacctgacc acacgcaaac ttatttagcg cggagaaacg gtcacctgac tgccattgct
    34381 ggccagtacg acacgttggc ccggagagaa acgagtgttg ccttgtttct gtaccaccat
    34441 gatggtatta ccatcgtctt tacgaatttc cagctcgaca ccctgcgttt tgttcattgc
    34501 actctgtacg ccctgaccag ctacgccacc tgcaacagcg cctgctgcag tagccagaga
    34561 acgcccggtt ccgccaccaa cagtattccc caggaaacca ccaagaacag caccgccaat
    34621 tgcaccgata acgttggaat catcaccgcc ctgaatctgt accggacgta cgttaacgat
    34681 ggtgccatag ctgacattct gtacttgttt cgcttcagaa gcggtataaa catcccctga
    34741 cagggtgtcg ttattaacac aaccgacaag agacagacct accattgaaa caaccaatac
    34801 gcgtttaatc attgaaaaat ctcctgttca ccatgaaacg ccacgcaagc atccctcatg
    34861 gtcaaagtat atggcatatg tgaatccatg gttacatgtt ctaccaaata tgagcaaatc
    34921 ataatgaaag taggatcaac caattataaa caaaacattc atgatgatta ttgatttgaa
    34981 gataatgttt tactaccaac ggtaaaaatc ggtcattccg catggtattc acgaaaagtt
    35041 tatgtttgag tggtgcgcaa acgtcagatt gaaaaaggat gaactatgaa atcgggccgc
    35101 tttattggcg ttatgtcagg caccagcctt gatggtgttg atgttgtgtt ggcgacaata
    35161 gatgaacacc gggtcgcaca gctggcaagc ttgagctggc cgatcccggt gtctctgaaa
    35221 caggctgtac tggatatttg ccagggccag cagcttacac tttcgcagtt tggacagctt
    35281 gatactcaac tcgggcgact ttttgctgat gcggtcaatg ccttgcttaa ggaacaaaac
    35341 ctgcaggcga gagatatagt tgcgatcggc tgtcacggtc aaaccgtctg gcatgaaccg
    35401 acgggcgtgg aaccacacac tttacagatt ggcgataaca atcaaattgt ggcacgcacc
    35461 ggaattacgg ttgtcggtga ttttcgccgt cgcgatattg ccttgggagg acaaggcgca
    35521 ccgctggtac ctgcgttcca tcatgccttg ctggctcacc caaccgagcg acgaatggtg
    35581 ctcaatattg gcggcatcgc caatctgtca ctgctcattc ctgggcagcc ggttggcggc
    35641 tacgataccg gtcctggtaa catgctgatg gatgcctgga tctggcgtca ggccggtaaa
    35701 ccttacgata aagatgccga gtgggcacgg gcgggtaaag ttattctccc actgctgcaa
    35761 aatatgctca gcgacccata tttctcgcaa cctgcaccga aaagcaccgg acgcgaatac
    35821 tttaactacg gctggctgga gcgccattta cgccattttc cgggtgttga tccccgtgat
    35881 gttcaggcca cactggcaga actcaccgcc gtgaccattt ctgaacaagt tttgttgagc
    35941 ggtggctgcg aacgattgat ggtatgtggt ggaggtagtc gtaatccgct actcatggcg
    36001 cgtctggcgg cattactgcc aggcacagaa gtcaccacca ccgatgccgt tggcattagt
    36061 ggcgatgaca tggaagcatt ggctttcgcc tggcttgcct ggcggacgct ggcgggatta
    36121 ccaggaaatc tgccttccgt cactggcgca agccaggaga cggtactggg ggctattttc
    36181 cctgctaacc cgtgatatca gagttaactg aatttttctt accgcgtctg ccgttacact
    36241 tggaacgtta gggagggcgt attgccctcc agaccaggaa agtcttcggg aaatgtctat
    36301 gaaaaaactg ttactgatct gtttgccggt gcttctcacc ggctgtagcg cgttcaatca
    36361 actggttgag cacatgcaaa ccgctacgct ggaataccag tgtgatgaaa aaccgttgac
    36421 ggtcaaactg aataatccgc gccaggaagt cagttttgtt tacgataatc aactactgca
    36481 tctcaaacag ggcatttcag cgtctggtgc acgttacact gacggaatct atgttttctg
    36541 gtcgaaaggc gatgaagcga ctgtctataa acgcgaccgc atcgtattga ataactgtca
    36601 gttacaaaat ccacagcgtt gagatttttc caggggcggc gcacaatagc gtcacccact
    36661 gacaatccgt aaagaaaacc atgtctgata acgacgaatt gcagcaaatc gcgcatctgc
    36721 gccgtgaata caccaaaggc gggttacgcc gtcgcgatct tcccgccgat ccattaaccc
    36781 tttttgaacg ttggctctct caggcgtgtg aagccaaact ggcagaccct accgcgatgg
    36841 tggtcgctac cgtggatgaa catgctcagc cttatcaacg catcgtttta ctcaaacatt
    36901 acgacgaaaa aggcatggtg ttttacacca acctcggtag ccgtaaagca catcaaattg
    36961 aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac ccttgaacgc caggtgatgg
    37021 tgatcggtaa agcagaacga ctttcgactc tcgaagtgat gaaatatttt catagccgcc
    37081 cgcgtgatag ccagattggt gcatgggttt cgaagcagtc cagtcgcatt tctgcccgcg
    37141 gtatccttga aagtaaattc ctggagctga agcagaagtt tcaacagggc gaagtgccat
    37201 tgcccagctt ttggggcggt tttcgcgtca gccttgaaca gattgagttc tggcagggcg
    37261 gtgagcatcg cctgcatgac cgctttttgt accagcgaga aaatgatgcg tggaagattg
    37321 atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta atcgctggta ctcctgattc
    37381 tggcacttta ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg gcaaaggtgc
    37441 agtcgttata tacatggaga ttttgatggc aagcagtaac ttgattaaac aattgcaaga
    37501 gcgggggctg gtagcccagg tgacggacga ggaagcgtta gcagagcgac tggcgcaagg
    37561 cccgatcgcg ctctattgcg gcttcgatcc taccgctgac agcttgcatt tggggcatct
    37621 tgttccattg ttatgcctga aacgcttcca gcaggcgggc cacaagccgg ttgcgctggt
    37681 aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa gctgccgagc gtaagctgaa
    37741 caccgaagaa actgttcagg agtgggtgga caaaatccgt aagcaggttg ccccgttcct
    37801 cgatttcgac tgtggagaaa actctgctat cgcggcgaac aactatgact ggttcggcaa
    37861 tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac ttctccgtta accagatgat
    37921 caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat caggggattt cgttcactga
    37981 gttttcctac aacctgttgc agggttatga cttcgcctgt ctgaacaaac agtacggtgt
    38041 ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc acttctggta tcgacctgac
    38101 ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt ccgctgatca ctaaagcaga
    38161 tggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga agaaaaccag
    38221 cccgtacaaa ttctaccagt tctggatcaa cactgcggat gccgacgttt accgcttcct
    38281 gaagttcttc acctttatga gcattgaaga gatcaacgcc ctggaagaag aagataaaaa
    38341 cagcggtaaa gcaccgcgcg cccagtatgt actggcggag caggtgactc gtctggttca
    38401 cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa tgcctgttca gcggttcttt
    38461 gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag gacggcgtac cgatggttga
    38521 gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat tctgaactgc aaccttcccg
    38581 tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc attaacggtg aaaaacagtc
    38641 cgatcctgaa tacttcttta aagaagaaga tcgtctgttt ggtcgtttta ccttactgcg
    38701 tcgcggcaaa aagaattact gtctgatttg ctggaaataa tgcattaagt ggaaaggggg
    38761 agtgagaaat cactccccct ggtttttata cagggaacat gatgaaaaat attctcgcta
    38821 tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc ggcagagttt cctatgcgcc
    38881 gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt ttctaatcac acccaatacg
    38941 gcaaatggac tggctgcgtg atgccgccca gccatttaac cgaaattgtg caaggcattg
    39001 ccgccattga taaattacac acctgtgatg ccgtgttaag tggctatctg ggatcggcgg
    39061 agcagggtga acatatcctc ggtatcgtcc gtcaggtgaa agccgcgaat ccgcaggcga
    39121 aatatttttg cgatccggta atgggtcatc cggaaaaagg ctgtatcgtt gcaccgggtg
    39181 tcgcagagtt tcatgtgcgg cacggtttgc ctgccagcga tatcattgcg ccgaatctgg
    39241 ttgagctgga aatactctgt gagcatgcgg taaataacgt cgaagaagcg gttctggcag
    39301 cgcgcgaact cattgcgcaa gggccacaaa ttgtgttggt taaacacctg gcgcgagctg
    39361 gctacagccg tgaccgtttt gaaatgctgc tggtcaccgc cgatgaagcc tggcatatca
    39421 gccgtccgct ggtggatttt ggtatgcgcc agccggtagg tgttggtgat gtgacgagcg
    39481 gtttactgct ggtgaaactg cttcaggggg caacgctgca ggaggcgctg gaacatgtga
    39541 ccgctgcagt ctacgaaatc atggtgacca ccaaagcaat gcaggaatat gagctgcaag
    39601 tggtggctgc tcaggatcgt attgccaaac cagaacatta cttcagcgca acaaagctct
    39661 gaaatatctc atttcggccc acaacgacct gtgggccgat ggatattact ttaagccttc
    39721 cgctgacagc gcgtcttgta cttccggacg ttcagccata cgttgcataa atgctgcaat
    39781 gtgctctaac ccttccagat tcagtttcac cgcgtatgcc cagcgcagaa ccgtaaacag
    39841 ataggcatca gcaattgtaa atctttgccc gcagatccag tgctcatcct tcagtgcctc
    39901 gttcacatat tgcagcttct tctccagctg cgcgcgaact gtcggtttgt actcttccgg
    39961 tgtatcaggg cgaaacagag gtgtgaaacc tttatgcagc tcggtggcga tgtaattcag
    40021 ccattcgatg gttttatagc gggaaatact gtttaccggt gccagcaact ggcggtcggg
    40081 gacgctgtcg gcaagatact gcataatcgc tacgccttcc gtcagcaaag taccgtcatc
    40141 cagcagcaat gcaggcacct gccccttagg gttaacggca aagtaatcgt caccgttttc
    40201 gagacgtttt ttcattaaat ccacactgac gagggtaaaa tcctttccgc tctcacgcag
    40261 ggtgatatgg gaagcgagag agcaggcacc cggtttgtag aacaatttca tcggtaactc
    40321 cttttgtctg tggtttaaag tatggtagtg cgttgctgaa caaaaaaaaa gccgctaacg
    40381 aaaagttagc ggcttgtaaa agagtttccc tgaattttac gctacagcag ctttcgccgc
    40441 tttgtctgca gcgtcatcct gcgtcatacg gtgcagttta ggcgcggtca gcagcatcag
    40501 tactgcaata atggcagtag cgacaccaat ctgcaagaat acgcgaccat aaacttccag
    40561 tgacatcagc gggtcggtaa cgttatccgg cacagccatc atacccgcaa cataaccacc
    40621 aatcaggttt gcaccggcag tggtcaggaa ccagctaccc ataatgaagc ccatcagacg
    40681 ctgcggaacg agttgtgcaa ccattgccag acccagacca gagatcatca gttccccgat
    40741 gctctgcagg ccatagcttg cgaccagcca gcttacagac acgataccag cgtcagacgc
    40801 gaatttcgca cccagcggca gaatcaggaa cgcaccagaa cacatcacca tgccgattgc
    40861 aaacttggtt ggcatcggca gggtatcgcc catcttgtta tagatagcgg ccagaatcgg
    40921 actaccgatg atgatccaga acgggttcag tgcctgatac tgctcaggtt ctacggccag
    40981 acccagaatg gagtgctcaa cgttacgaat cgcaaagaag ttcagtgatg tcggcatctg
    41041 gctgtacagc acgaagaaga tgattgcttc gagcatcagg atgaaggcaa cgatcatttt
    41101 acgacgcgca gcacctttca tcgcgaaggc ttctttaccg aagataacca cgataccgaa
    41161 ggcaacaacg cccagcgcca tacgcgcaac ttcctgattg tgcagcagcc aggtggcgat
    41221 agcgatcagt gccacaacac caataatggt tagcagcagg ttacggtagt tgataggctc
    41281 gaagtctggt tttgaaccgt actgtttaac ccagcgttgg cagaaggcga agttaacgat
    41341 agtgatcagc aggcctacaa cgctcaatgc aaacgcaacg ctccagccgt atttcgcggc
    41401 cagccacggc gtagcaatca tagagaagaa agagccgatg ttgacggaca tgtagtacat
    41461 ggtgaatgca ccgtccagac gcgggtcgtt tttctcatag catgtagaaa gcagagaaga
    41521 cgggttagcc ttaaacaggc cgttaccgac cgcaatagcc gccataccca tataaacaat
    41581 accggcgtcg tgaccagacc aggcaaccag agcataacca atcgccagca caatagcgcc
    41641 gagcataatt acgcgtttag tacccagtac cttgtcacct aaccagccgc cgatagcgac
    41701 cagaccataa accagggcac taaaggaaga gaaaagggtg attgaatccg cttcagacat
    41761 acccagttgt ttaaccaggt aaacagccat aattccttgt aggccgtaat aaccaaaacg
    41821 ttcccataac tcaatcgaga agatgagata gaacgccttc ggttgtttga aagcgttcaa
    41881 actgacgctt tcagttggtt tttggtttgc agtggacact tttacctctt tttttacatc
    41941 ccatattaac gggggtgctt tctcgcgtta tgtctaaaaa acatacgcgc aattgttata
    42001 gtgggagggg aaacggcagg taatgttcac tatcttgcac tttcagacaa gtcctttgta
    42061 ataatctgtt acatataact gaggcgggga attcaaccat tccgggaaat gttatccagc
    42121 gttaaatttt tcaaaaaacg taacggcaga ttttttcact gcactgagag gggggtgatg
    42181 gcttaacggc gatatgttct gctatttagc gtttttacta gtgagatagt ccagtttctg
    42241 aaaaatagcc agtgtaatgt tttgtaggtc aatatttgtt gcgtgtgtaa atttaaatga
    42301 ttttaacctg atttttgcaa tgtttttaca ttgccacagc atcgtgatct tgatcacgaa
    42361 tgaatagaaa atttcgctat taaaaaaaca attaacctgc atttttggtt atccgcaaac
    42421 ggattacctg atgataacgc taagcaatgg catcattcac gttaaaaaga tgttttaaaa
    42481 gaggataaag aaaggttatc aatggggtaa tcggtgttac cccttttctt cagatgtcaa
    42541 ctttctcttt gtattcacaa agatcttcaa taatacaaga gccacagcgg ggcttgcggg
    42601 caatgcaggt ataacgcccg tgcaggatca accaatggtg gcagtcgact ttaaactctg
    42661 ccggaaccac ttttagtagc ttttcttcta cctgttcgac atttttcccc ggcgcaaatt
    42721 gagtacgatt acaaacgcgg aaaatgtgcg tgtcgacagc aatagtcggc cagccgaatg
    42781 cagtgtttaa tacgacgttg gctgttttac gacctacgcc gggcagggct tcaagcgcag
    42841 cacgatcttc cggaaccaag ccattatgct gctccagtaa gatacggcag gttttgatga
    42901 tattttctgc tttgctgtta taaagcccaa tcgttttgat ataggtttta accccttcaa
    42961 cgcccagttc aagcatcgct gcaggcgtat tcgccaccgg gtagagtttc gccgtcgcct
    43021 tattaacact gacatcggtc gcctgagcgg aaagcagtac ggcaatcagc aattcaaaag
    43081 gcgaactgaa attaagctcg gtggtgggat gaggattgtt ctcacgcagg cgagtgagga
    43141 tctccaggcg ttttgctttg ttcatcagac attccctgtt tcaccgtttg gcaatgcacg
    43201 ttcggcagct gcttcagtac ggcgcttttt cattctttca tcaatcaggt attttcctgc
    43261 cagcatcagt cccaggccaa taaatgcacc tggaggcagc atcgccagca ggaaagggga
    43321 gtcggtgtgg aaaatttcca cgcgtaatac ttttgcccag ctacctaaca gcgcatctgc
    43381 accgtcaaac aatgtgccat tgccgataat ttcgcgtagt gaacccagca cgaacatggc
    43441 gcaggttgcg cccataccaa ttgaaaagcc gtccagtgcc gaaagcgccg gacctttttt
    43501 ggcggcgaag gcttcagcgc ggcccacaac gatacagtta gtgacaatca gcggaataaa
    43561 aatccctaat gattgataca ggccaaaggc gtaggcgttg atcagcatct gtacagcgct
    43621 gaccaccgag gcgatgatca tcacgtaaat gggaatgcgg atctcggctg gcgtccagtg
    43681 acgcagcgtc gaaatggtca ggttggtcag cgtcagtacc agcgtagtcg caagtcctaa
    43741 acccagagcg ttagtggcag tggacgtgac cgccaacaga ggacaaaggc cgagcaactg
    43801 gaccagcgca gagttgtttt tccacaaccc ctgaacaata acgtctttaa tttcgctcac
    43861 gctttattct ccacaggcag gaagttgaga aagttgtgcc ggtaacgtct gagcgtacaa
    43921 tccggcgcgt tttaccgcat taaccaccgc gcggggagta atcgtcgcgc cggtgaactg
    43981 gtcgaaatca ccaccatctt tcttcaccgc ccagtgcgca tcatctgcac cactgatttt
    44041 tttacccgca aaatgggtga tccagtcaga aaggcgcagt tcgattttat cgccaagccc
    44101 tggcgtttcg tggtgctctg tcacgcgcgt gccaagtacc gtgccgttaa aatcggctcc
    44161 caccagcagc tgaatcgcac cggaatagcc atctggcgcg gttgcttcca gaacggcggc
    44221 taccggtttg tcatcctgtt tggcgatgta aacccgatgc tcacctttac ctaactctgg
    44281 cgcagttacc agatagcaac tctgtgccag cgcattgtta tagcgttcgg ctggcagcac
    44341 ctgatcaaat aatgcctttt gttgcagact ggcctgttca gcaatcgtcg ttttggtcat
    44401 ctggttgatg gccgcagtta accctgttga acccgctgca aatagcgcta acgtaatgcc
    44461 gtgttttcgg atagttttca gcatggttta ccctttgcga tggccgtaga cgcgcggacg
    44521 cgtgtagtaa tcgatcagag gaaccgtgat gttcgccagc aggacggcaa aagccacgcc
    44581 gtcaggatag ccgccgaaac tgcggatcaa ccagactaat aagcccgcca gcgcgccgaa
    44641 aataagacga ccacgattgg tcgtagaagc ggtaaccggg tcagtcaaaa taaagaatgc
    44701 gccgagcatg gtcgcaccag acagcagatg aatttgcggt gctgccagtg tttctggtga
    44761 gaacaaccag cccaacgttg cgcataacgc cagcgttact aagaagctga ggggaatatg
    44821 ccagcgaatc gctttctgcc atagcaacca caggccgcca gccagccagg cgagatttac
    44881 ccattgccaa ccagcgcccg ccagaatacc gctgtagatc ggatattgca taatctgttc
    44941 aaccgaatga ccggcacgga cagaggtttt aaatgtatcc agcggtgtcg cctgactaat
    45001 gccatcaata cctaagcgta gtgtgttcat atcaccacca ctggcggtat gtccgctgaa
    45061 aataacctgt atggcgtcga taaaaccagg gatgttgacc gcaatttcat gtggcggtaa
    45121 ccagctggtc atctgcaccg ggaaggagat cagtaagacc acataaccaa tcattgccgg
    45181 attaaacggg ttttgcccca gaccgccata caactgttta gcgatgatca ccgcaaacac
    45241 cgtacccagc acgaccatcc accatggcgc gaggggggga atacttaccg ccagcaataa
    45301 gcctgtcagc aatgctgagt tatctttcaa cgttgcggct accgactgct tgcgtagttt
    45361 gagtacgaga gcttcggcta acagagcact aaccgatgcc aacaggatct gaacgagagt
    45421 accccaacca aaaaaccaca gttgcgctgc gattcctggc acggctgcga gcaacaccaa
    45481 cagcataatg cgcgatgtct ggcgctggtt atgggtataa ggggagctag ctattctgaa
    45541 taccatttag tcctcgttta caaccttctg ctgggcggct tttttggcct gagcgcgggc
    45601 aatagccgcg gcaactgccg ctttgcgcgg atcaacctgt tcttctggtt ctgcgttagt
    45661 ctgttgctgt tccagcttgc gcgctttggc acgggcaata gcggcttcga cggcggcttt
    45721 gcgcggatca acctgttctt ctggttccgc attagcctgt tgctgttcca gtttgcgcgc
    45781 tttggctcgg gcaatagcgg cttcgacggc ggctttgcgc ggatcaacct gttcttctgg
    45841 ttccgcatta gactgttgct gttccagctt gcgcgctttg gcacgggcaa tagcggcttc
    45901 gacggcggct ttgcgcggat cgacctgttc ttctggttcc gcattagcct gttgctgttc
    45961 cagcttgcgc gctttggcgc gggcgatagc tgcttcaacg gcagttttac gtggatcagc
    46021 aacggttgct gcgtcgttag tttgctgcag ttctgcctgt ttcgctctgg cttgcgcttt
    46081 acgggcttcc cgtgctgcaa taattgcact gttatccggg cgttcgcccg ctttaatcac
    46141 aataggctgt gtagcctggg cctgtttctc tttcacccgc gccagagcgg cagcaatcgc
    46201 atctttatct ttggctgcag gttgaacggc tgcgctctta tgtcgttcaa ggcgagccgc
    46261 tttttcgcgc tccagacgag cctggcgcgc ttcgaaacgc gctttggctt ctgcggcgcg
    46321 cttttcttcc tgacgaatag ccgcaatttc agctttttcc tgacggaaat attgcaccag
    46381 gggaatattg ctcgggcaaa cccacgcgca agccccacat tcaatgcaat cagcaatgtt
    46441 atgcgtggta gctttatcgt gttgctgacc tttgctgaac cagtacaact gttgcggcaa
    46501 aagatccgca gggcaggcgt cagcacaggc gctacaccgg atgcagcttt gttcttcctg
    46561 tggttcgcca agttcattgg cagagggagc caacagacag ttggtaatct ttacgaccgg
    46621 gacatccagc catggcaagg taaagcccat tagcgggcca cccataatca ccatttgatc
    46681 ggcagagggg cagaatccgg catcattcaa taaatgacgc actggcgtcc ccagccgtgc
    46741 ccagacgttg cccgggcgag cgattgcttc gccagtcagg gttacaacac gctcggtaat
    46801 cggctcgcca tcaataacgg cacgtttcac tgcataagca gtgccgacgt tttgcattaa
    46861 tacgccgata tcggatgaac gcccgccatg tggaacctgc ttcccggtca gaatgtaggt
    46921 taattgttta gcaccgccag aaggatattt ggttggaatc acccgcagag aaatatcgtt
    46981 agagtccgcc agcaccgcgc gcagcatgga aatcgcctgc ggtttgttat cttcaatgcc
    47041 gataagaatt tcgcgtggct gcagaatatg cgcaagaatg cgaataccct ctacgacctg
    47101 agccgcgcaa tcctgcatca aacggtcatc ggcggtaatg tacggctcgc actcagccgc
    47161 gttgataatc aacgtttcaa tcttatctcc gccaccctgc aatttaacgc ctgtcgggaa
    47221 tcctgcaccg cccagcccgg caacaccaaa ctgatgtatg cgctcgatta actcttcgcg
    47281 actgcgagtg cgataatcgg cccagccgtc gcgcgggatc cagcagtctt caccatcggc
    47341 atcaataatc acgcttaatt cagctaaagc tgaaggatga gccgtagagt ggggcgcaat
    47401 agccgtaacg gtacccgagg tgggcgcgtg aacaggcagc attttgccgc gaccacgggt
    47461 aagcggctgg ccgcgcaata ctttatcgcc gacgctaacg cacaactcac cttcagcgcc
    47521 aatatgctgt ttcagtggaa taacaaaacg ctgcgccagt ggtacctggc gcaggggtgt
    47581 accgttggac tgggttttca tctccggtgg atggatgccg ccgttgaaat cccagatttt
    47641 attttttctg aatgcagaga ataacttaag catggtgttc cacgggaatg atacgcacgg
    47701 gaatggtgtt cagatcccat ttccaggagt caggtgtttc tgcgaccggt tgcaacgaga
    47761 tgcagtgcgt cgggcacgga tcaacacata aattgcagcc cgtacagaga tcactcatta
    47821 ccgtatgcat ggcacgggta gcgccaacga tggcgtctac cggacacgcc tgaatacatt
    47881 tagtgcagcc aatacagtta ttttcatcaa taaccgccac catccgcgca ggcgttatct
    47941 cttgcgcttc gccatccagc ggctgcggct cgacattaag caactcggca atttttagca
    48001 tcacagcttc gccacctggg gcgcaacggt tgattttttc accgttacag ctgatggctt
    48061 ccgcgtaggg gcgacagccg ggataaccgc actgaccaca ctggctctgc ggtaagattt
    48121 cgtcaatttt ctcaacgacc ggatcgtctt ccaccgcaaa acggcgggag gcataaccca
    48181 gaatggcgcc aaacgccagg cccagcaggc tcacggcggc aacggcaatc cagatagcat
    48241 tcattacaac ttcaccaaac cactaaagcc cataaaggcc agagacataa gacctgcggt
    48301 aattaacgca atggcatgac cgcgaaaagg tgccgggaca tcagccacag caaggcgttc
    48361 gcggatggcg gcgaagagca ccatcaccag cgagaaaccg atagcggcgg aaaaaccgta
    48421 cagcgccgac tgcaagaaat tgtgcccgag attgatattc agcaacgcca cgccgagcac
    48481 tgcacagttg gtggtgataa gcggcaaaaa aatccccagc agacggtaaa gcaccgggct
    48541 ggttttgcgc accaccatct cggtgaactg cacgaccaca gcaatcacca gaataaatgc
    48601 cagggtgcgc aggtaaatca gattaagtgg gatcaaaatc cacgtatcga taagccaggc
    48661 gcaaatagac gccagcgtca tcacaaacgt tgttgccagc cccatgccca tcgcggtttc
    48721 cagttttttg gaaaccccca taaacggaca gagaccgaga aacttgacca gtacaaagtt
    48781 attgaccagt acagttccga caaacagtaa caggtagtca gtcatttttc ggcctgaaat
    48841 aaaaaagccg cctattatcc gttaatccag agcaggcgac aacaggttaa ctgtaaggtt
    48901 attacggatt cacaaacgtc gcttttaccc gcgacgagcg tttgaaatag gggacgatca
    48961 atgcggttgc caacaaagta aacagcaact ggcgaacggc tatgccatct tccacgggtg
    49021 aaaaagcgaa tgcttttact gccagcaata cggaaataag cagccagata atatagtgtt
    49081 taggaacaca acgccgacgt ttaaagaaag cgatggtcaa ccacagtgtg taataccaca
    49141 tggcgatagc ggtaataaaa gaaacgcccc ataagatttg tgtcgtcagt gcttgtccgc
    49201 cgagcgtttg aaatgtttgc ggagaagata acgcagcggt atacagcaac aacgccagcg
    49261 tcgtactcaa cagagcgact aaaagccagg ccaacgggcc aagcaaccag cctccaatac
    49321 gttgtggcgt tgtggtggtc ataggttctc cagaatagcg acgcaaacag gcaaatctaa
    49381 ttttaggggc gttattataa ggcttttata accgatcgct actttcttta ttggacatag
    49441 tgccagacgg acttcggtac ctggccgagg tcaaacaaac gcccgccaga aaccagctct
    49501 gcgcgacggt gatcggcagc acgatacata ttgatcagtt cctgatcgtc cgtcagggtg
    49561 taattaagat ggtcgtagag tttttccaga ctttcgagtg aactgatttt gcgaaatttt
    49621 aataagtagt cctgaacagt cataaaaggt ccataaaata attaatacca aaggggtagg
    49681 tattattagt gatgataaaa tttcaccaaa agtgaacgct tgtgttatta caataataag
    49741 aatgcgctga tggcaaatat tcagggatga aaaactgctc tgaaaagagt aacgcgcttt
    49801 actatccctg acaatcacca acaacatcga acaagataat aaattcctgg tttaatatcc
    49861 gacaagtgaa aacatgcacc cggacgggca gcatgtcgct ccacaagtgc agagcttact
    49921 tgtgttgtac cgaagcactc tgttcaggtg gctgatagtt gtcaatgtga ctcgccacgc
    49981 caagaagaat gactgaaacg acaagaacga tccaacctgt taattcaata agacgattca
    50041 ttacagccca catctctctt gattgatcca ttaacttcag ggggtaaatg ttacttagca
    50101 ataatagctc cagcaagatt tttactgagg tttttgcgat atttagcttt tgtcgttgga
    50161 aaattcgcta tttttttgac ttaagttaaa caacatccct tattgctggc aggttattaa
    50221 actgttgagc gtgggtaaag gatagtgtca aatagccatc atacttcaat gagaggcaat
    50281 gacatgagcg acaacatccg tgttgggttg attgggtatg gttatgcgag caaaaccttc
    50341 catgcgcccc tgattgcggg cacgcccggg ctggaactgg cggtaatctc cagcagtgat
    50401 gaaacaaaag taaaagccga ctggccaacg gttacggttg tctctgagcc gaagcatctg
    50461 tttaacgatc ccaacataga cctgattgtc attcctacac ccaacgatac ccatttcccg
    50521 ttagccaaag cggcgcttga ggcgggtaaa catgtggtcg ttgataaacc ctttaccgtg
    50581 acactgtcac aagcgcgaga gctggatgcg ctggcaaaaa gcctggggcg tgtgctgtct
    50641 gtattccata accgtcgctg ggatagcgat ttcttgacgc taaaaggttt actcgcggaa
    50701 ggcgtgctgg gtgaagttgc ttactttgag tctcattttg accgcttccg tccgcaggtg
    50761 cgcgatcgtt ggcgtgaaca gggcggccca ggcagcggta tctggtacga tttagcacca
    50821 catcttcttg atcaggccat tacgcttttt ggtttaccgg tcagcatgac ggtagatttg
    50881 gcacagttac ggcccggagc gcagtcgacc gattatttcc acgccatctt gtcctatcca
    50941 cagcggcgag tcattttaca cggtaccatg ctggcagccg ctgagtcagc acggtatatc
    51001 gtgcatggat cccgaggcag ttatgtgaaa tatggcctcg atccacagga agaacgtctg
    51061 aaaaatggcg agcgtctgcc gcaggaagac tggggctacg atatgcgtga tggcgtactt
    51121 acccgcgtgg aaggtgagga acgtgtcgaa gaaacgctgt tgacggtacc tgggaattat
    51181 ccggcttact atgcggctat tcgtgatgcg ttaaatggcg atggtgaaaa tccggttccg
    51241 gcaagccagg caatccaggt aatggagttg attgagctgg gcatcgaatc cgccaaacat
    51301 cgcgcgactt tgtgccttgc atgaaaaatg gatggtgcaa tttgcaccat ccattttgtt
    51361 acttcgcggc gactttttct cgcagtgcgc gtttttcctc agcgctgagg aaagccattt
    51421 ccagaccatt aatctgtgcc tggcggattt gctcgcggga taacccagca gctggcgcgg
    51481 caacggtata ttcgtgaatg atatccactc cctgtacgcc gggatcgtca gtgttaatgc
    51541 tggcacgaat gccatgctca aggaacgttt tcagcggatg tgcagccagc tctgctacgg
    51601 tgctggtctg aatattggag gtcagacagg attcaatacc aatttgttgc tcggcgagaa
    51661 aatccatcag cgcccgatct tcaatggctt ttacgccatg tccaatacgc tccgcaccca
    51721 gttcacgaat cgcctgccag atgctttccg gcccggcagc ttcgcctgca tggacggtaa
    51781 tatgccagcc cgcatcacgc gcgcggttga agtgagaaag gaacagactt cccgggaaac
    51841 caagttcatc accggctaaa tcaagtgcgg taatctggtc acggtgggct aaaaaggcct
    51901 ccagctcttg ctgacaggcg gcttcgccga aggtccggct cataatgccg ataagcttcg
    51961 cctgcacacc aaaggtgcgg caaccttcac gtacgccatc gatcaccgct tcgacaacac
    52021 ccgctacagg cagctgatgt gccattgcca tgtagcctgg tgaaaaacgc agctcgacat
    52081 agtgcaggcc gtgacgggct gcatcttcaa tgttttcaaa tgccacgcgg cgacaggcat
    52141 caagagaggc gagaacttta acgccccagt caagtttggt cagaaagctc accagatcgg
    52201 gttcgttggc aatgacctga acgtggggaa tcagtgtttc cagggattgt gcaggaagcg
    52261 agatattata ctggcggcca agttcaagaa tggtctgggg acgaatgttg ccatcaaggt
    52321 ggcgatggat atcagttaat ggcagggtgg tatcaatcat ggtcgcactc tttttctgaa
    52381 tataaaagtg cgcaccatta taaaaacaaa gcagggtaaa aatccacgat tgcgcaacga
    52441 ttttttacgt tgcgcaattg gggttagcga acagcgcgga tggcgtttat taatccagcc
    52501 acaccttttt ccagtttcga acgtgggcag ccggcattga gacggacaaa accacgacct
    52561 tcttcaccat aggtataccc cggcatgatc gcgacttttt cttgctcgat aagcgctttt
    52621 tgcaacgcgt tgtcgtcaat attcaacaga cgtagatcaa gccaggccag ataagtggat
    52681 tgcgggatct gccagttgag ttcaggaaac gcggcgttca ttttatctga gatatacgtc
    52741 aggttatctt tcagatagac gcgtaaggca tccagccacg gtgcaccttg ctgataggcg
    52801 gcgatatggg cagttaacgc cagtaccgaa ggggaagaaa gcccatcacg tcctttcagt
    52861 gccgataaat aggcatcacg gctgctgcta ttttctataa tcccgtaagc accggtcagc
    52921 gcgggaatat tgaaactttt cgagcccgac gtcagcaacg cccagtctcc ccgcgccaca
    52981 ttactccagg gaatatgcgg ctgctcgccc cagaccatat ccatatggat ttcatcggaa
    53041 ataacccgca caccatgacg ctcgcatagg tcagccatga tctccagctc atcgcacgtc
    53101 cacactttcc cggtaggatt ctgtgggcta cacaggagca taattttaca ttctggtttc
    53161 gccaacacgg cttccaactt gcccatatcg caaaaccaac catcagcctg cttctctaaa
    53221 gcaacgggca ttactgtgcg ctggttacct tcaatggcct tgtaaaatgc gtcataggcg
    53281 ggtgtgtgga tcaccacgcc ttcacctgtt tcagaccact gacgaatcag ttctgaaacc
    53341 atatagatga cagaagggcc atacaccacc gtctgagaat cgatggcggt gtaatgctgg
    53401 gtggaaaacc agtgggcaat agccgcgaga aactcatcgt ttttccagcg gctgtagcca
    53461 aatacgccgt gcatcaggcg ctgattcagc gcctcgataa tgcagggggc agtggcaaaa
    53521 tccatgtctg aaatcgtgaa cggtaacagg tcagcagtgc cgaaacggtc agcgacataa
    53581 tcccactgtg tacaccatgt gccatgacga tccacgacct ttgaaaaatc gaacatatct
    53641 tatccttatg cctggacagt atgcatcaga ccggccattt catcttttac tgactgaact
    53701 tgtggcccga taacaacctg caggttatgt tgattaagtt gtactacgcc aattgcccga
    53761 ttgtccttca gtgccagcac attaacaagc gacatatctt tcacagacaa acgcagacgg
    53821 gtaatgcagt tatcgaggct gacaatattg tcggcaccgc ctaatgcttc gaggattgca
    53881 ggaacgttgt aacctgattt acccggcgca ccggcaacgg ctttttcgat tgagctggca
    53941 acttcgctat cgcgccccgg ggttttcaga ttgaagcggg tgatagcgaa acggaagatg
    54001 acgtagtaaa cgacaaacca gattgccgcc accactggca ccatgtacca cttggttgac
    54061 agaccatgca aaataccgaa caccacgaag tcgatgatat tgccgtcggt attaccgatg
    54121 gtgacgccga gcacagacat gacggtgaag ccgaggccgg ttaacagcgc gtggatgaca
    54181 tacagaactg gcgctacgaa caggaacagg aattccagcg gttcggtagt gccgccaacg
    54241 acgcaggcga tcaggccaga aatcagcaga cctttaattt tatggcgatt ttccgggcgc
    54301 gcgcagtgat acatagctaa cgctgcacct ggcagaccgc cgagaaacgc aggcatttta
    54361 ccttgcgaaa ggaaacgcgt ggcgctttca gaaaaaccgt gagtggtcgg gcaactcaat
    54421 tgcgcctgga agatggtcaa tgcgccgctg acggtttgac cgcagacttc ctgcgtgccg
    54481 cctgcgtcgg taaagcgaat taatgccacc agaatgtgat gcagaccaaa cggcaacagc
    54541 agacgttcac cggtaccaaa cagcatcggt ccgaaatcac ccgcgctgtt tatcatatgg
    54601 cccaagccgc taatacccat cgcgaaaatc ggccagacta atggaatcac caggccgaca
    54661 aggcccatca ccagcgagga gatgattggt acgaagcgcg taccgccgaa gaatgcaagc
    54721 gcatccggca ggcggatatt atggaatcgc tcatgcagca tccagacgat aataccggcg
    54781 atcaccgcac cgaggatacc ggtgtcgatc gactggatcc caagaatgct ctggatgtta
    54841 ttggctttca gaaccgcggc gtccgtggtt ggcagaatgc ctttattggt caaccagaag
    54901 tttaccgcga ggttcattac cgcataaccg acgaagccag cgaatgccgc tacgcctttg
    54961 ttttcgcgtg ccagacccag cgggatggcg atacagaaca tgactggcag gaaactaaaa
    55021 gcaaacgagc caatcttact catccaggta aagatagcct gcaacacggg gttgcccagg
    55081 accgggatca gggtgatgac atcgtggcta ctaagagaac taccaatgcc gagcataatg
    55141 ccgcagaacg acaataatgc cacgggtaac atgaaggttt tgcctaactg ctggaagaac
    55201 tcccacagcg tgactttcgg tgctgttttc gccgtcataa aacgactcct cgtagagaac
    55261 aatctggatt cagtaaattg cgcgagaaga taaaacgttt tatcaaattt tagtgaggca
    55321 taaatcacat tacgcaacga taatagcgag tataagataa ataaaaggta aaacgtttta
    55381 tctgtcacat aatcagggag taggtcatct gcatggctac cgccaaaaaa ataaccattc
    55441 atgatgttgc gctggctgcg ggcgtgtcgg taagtaccgt ttcgctggtg cttagtggca
    55501 aagggcgaat ctctaccgcc acaggagaac gcgttaacgc cgccattgaa gagctgggat
    55561 ttgtgcgcaa tcgccaggcg tcggcgctgc gcggcgggca aagcggcgtc attggtttga
    55621 tcgtccgtga tttatctgcg ccgttttacg ccgaattgac ggccggattg acggaagctc
    55681 tggaagcgca gggacggatg gtttttttgc ttcacggcgg taaagacggc gagcagctgg
    55741 cacagcggtt ttcactgtta ctgaatcagg gtgtggatgg tgtggtaatt gccggggctg
    55801 caggaagcag cgatgacctg cgacggatgg cagaagaaaa agctatcccg gtgattttcg
    55861 cttcccgtgc cagttatctt gatgatgttg atacggttcg cccggacaac atgcaggctg
    55921 cacagttgtt gacggagcat ctcattcgca atgggcatca gcggatcgcc tggctgggag
    55981 ggcaaagttc ctcattaacc cgtgcagaac gggtgggggg ctattgtgca actctactaa
    56041 aatttggcct gccgtttcat agcgattggg tgttggagtg cacttccagc cagaagcaag
    56101 ccgcggaagc tatcacggcg cttttacgtc ataacccgac catcagtgcc gtggtttgct
    56161 ataacgaaac tattgcgatg ggggcatggt ttggtttgct caaagcaggg cggcaaagcg
    56221 gggaaagcgg agtcgatcgt tactttgagc aacaggtttc gctggcggca tttaccgatg
    56281 cgacaccaac cacacttgat gatatacctg ttacctgggc cagcacgcca gcgcgggaac
    56341 ttggtatcac acttgcggat cgcatgatgc aaaaaatcac ccatgaagag acgcattcac
    56401 gcaatcttat tattcccgcc cggctcattg cggcgaaata atcctctctt tatctgctat
    56461 acctggtagt atcctttcct caaggttaat gataaaaaat gtggcacaca ggcggtgcct
    56521 tttattttta ctgaagttca caggaggttt atgtgtttaa ttctgacaac ctgagactcg
    56581 acggaaaatg cgccatcatc acaggtgcgg gtgcaggtat tggtaaagaa atcgccatta
    56641 cattcgcgac agctggcgca tctgtggtgg tcagtgatat taacgccgac gcagctaacc
    56701 atgttgtaga cgaaattcaa caactgggtg gtcaggcatt tgcctgccgt tgtgatatta
    56761 cttccgaaca ggaactctct gcactggcag actttgctat cagtaagctg ggtaaagttg
    56821 atattctggt taacaacgcc ggtggcggtg gacctaaacc gtttgatatg ccaatggcgg
    56881 attttcgccg tgcttatgaa ctgaatgtgt tttctttttt ccatctgtca caacttgttg
    56941 cgccagaaat ggaaaaaaat ggcggtggcg ttattctgac catcacttct atggcggcag
    57001 aaaataaaaa tataaacatg acttcctatg catcatctaa agctgcagcc agtcatctgg
    57061 tcagaaatat ggcgtttgac ctgggtgaaa aaaatattcg ggtaaatggc attgcgccgg
    57121 gggcaatatt aaccgatgcc ctgaaatccg ttattacacc agaaattgaa caaaaaatgt
    57181 tacagcacac gccgatcaga cgtctgggcc aaccgcaaga tattgctaac gcagcgctgt
    57241 tcctttgctc gcctgctgcg agctgggtaa gcggacaaat tctcaccgtc tccggtggtg
    57301 gggtacagga gctcaattaa tacactaacg gaccggtaaa caaccgtgcg tgttgtttac
    57361 cgggataaac tcatcaacgt ctctgctaaa taactggcag ccaaatcacg gctattggtt
    57421 aaccaatttc agagtgaaaa gtatacgaat agagtgtgcc ttcgcactat tcaacagcaa
    57481 tgataggcgc tcacctgaca acgcggtaaa ctagttattc acgctaacta taatggtttt
    57541 atgatggata acatgcagac tgaagcacaa ccgacacgga cccggatcct caatgctgcc
    57601 agagagattt tttcagaaaa tggatttcac agtgcctcga tgaaagccat ctgtaaatcc
    57661 tgcgccatta gtcccgggac gctctatcac catttcatct ccaaagaagc cttgattcag
    57721 gcgattatct tacaggacca ggagagggcg ctggcccgtt tccgggaacc gattgaaggg
    57781 attcattttg ttgactatat ggtcgagtct attgtctctc tcacccatga agcctttggg
    57841 caacgggcgc tggtggtcga aattatggcg gaagggatgc gtaacccaca ggtcgccgcc
    57901 atgcttaaaa ataagcatat gacgatcact gaatttgttg ccgagcggat gcgtgatgcc
    57961 cagcaaaaag gcgagataag cccagacatc aacacgtcaa tgacttcacg tttactgctg
    58021 gatctgacct acggtgtact ggccgatatc gaagcggaag atctggcgcg tgaagcgtcg
    58081 tttgctcagg gattacgcgc gatgattggc ggtatcttaa ccgcatcctg atcctctcat
    58141 ttcgcgggct ggtgataact gtgcccgcgt ttcatacttt aatttccctg tacaaaatac
    58201 aatctagccg ggatcggaga attccccccc aaaaatattc accgtagcca tatgtcatga
    58261 gagtttatcg ttcccaatat actcgaacga acgttcggtt gcttatttta tggcttctgt
    58321 caacgccgtt ttaaagatta atgcgatcaa tatcacgctg tgggtattgc agtttttggt
    58381 tttttgatcg cggtgtcagt tctttttatt tccatttctc ttccatgggt ttctcacaga
    58441 taactgtgtg caacacagaa ttggttaact aatcagatta aaggttgacc agtattttta
    58501 tcttaatgag gagtccctta tgttacgtcc tgtagaaacc ccaacccgtg aaatcaaaaa
    58561 actcgacggc ctgtgggcat tcagtctgga tcgcgaaaac tgtggaattg atcagcgttg
    58621 gtgggaaagc gcgttacaag aaagccgggc aattgctgtg ccaggcagtt ttaacgatca
    58681 gttcgccgat gcagatattc gtaattatgc gggcaacgtc tggtatcagc gcgaagtctt
    58741 tataccgaaa ggttgggcag gccagcgtat cgtgctgcgt ttcgatgcgg tcactcatta
    58801 cggcaaagtg tgggtcaata atcaggaagt gatggagcat cagggcggct atacgccatt
    58861 tgaagccgat gtcacgccgt atgttattgc cgggaaaagt gtacgtatca ccgtttgtgt
    58921 gaacaacgaa ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg
    58981 caagaaaaag cagtcttact tccatgattt ctttaactat gccgggatcc atcgcagcgt
    59041 aatgctctac accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc
    59101 gcaagactgt aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt
    59161 tgaactgcgt gatgcggagc aacaggtggt tgcaactgga caaggcacta gcgggacttt
    59221 gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt
    59281 cacagccaaa agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt
    59341 ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg
    59401 tcgtcatgaa gatgcggact tgcgtggcaa aggattcgat aacgtgctga tggtgcacga
    59461 ccacgcatta atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc
    59521 tgaagagatg ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc
    59581 tgtcggcttt aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact
    59641 gtacagcgaa gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga
    59701 gctgatagcg cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc
    59761 ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa
    59821 actcgacccg acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga
    59881 taccatcagc gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca
    59941 aagcggcgat ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga
    60001 gaaactgcat cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca
    60061 ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca
    60121 ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt
    60181 tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga
    60241 ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc tggactggca tgaatttcgg
    60301 tgaaaaaccg cagcagggag gcaaacaatg aatcaacaac tctcctggcg caccatcgtc
    60361 ggctacagcc tcggtgacgt cgccaataac ttcgccttcg caatgggggc gctcttccta
    60421 ttgagttact acaccgacgt cgctggcgtc ggtgccgctg cggcgggcac catgctgtta
    60481 ctggtgcggg tattcgatgc cttcgccgac gtctttgccg gacgagtggt ggacagtgtg
    60541 aatacccgct ggggaaaatt ccgcccgttt ttactcttcg gtactgcgcc gttaatgatc
    60601 ttcagcgtgc tggtattctg ggtgccgacc gactggagcc atggtagcaa agtggtgtat
    60661 gcatatttga cctacatggg cctcgggctt tgctacagcc tggtgaatat tccttatggt
    60721 tcacttgcta ccgcgatgac ccaacaacca caatcccgcg cccgtctggg cgcggctcgc
    60781 gggattgccg cttcattaac ctttgtctgc ctggcatttc tgataggacc gagcattaag
    60841 aactccagcc cggaagagat ggtgtcggta taccatttct ggacgattgt gctggcgatt
    60901 gccggaatgg tgctttactt catctgcttc aaatcgacgc gtgagaatgt ggtacgtatc
    60961 gtggcgcagc cgtcattgaa gatcagtctg caaaccctga aacggaatcg cccgctgttt
    61021 atgttgtgca tcggtgcgct gtgtgtgctg atttcgacct tcgcggtcag cgcctcgtcg
    61081 ttgttctacg tgcgctatgt gttaaatgat accgggctgt tcactgtgct ggtactggtg
    61141 caaaacctgg ttggtactgt ggcatcggca ccgctggtgc cggggatggt cgcgaggatc
    61201 ggtaaaaaga ataccttcct gattggcgct ttgctgggaa cctgcggtta tctgctattc
    61261 ttctgggttt ccgtctggtc actgccggtg gcgttggttg cgttggccat cgcttcaatt
    61321 ggtcagggcg ttactatgac tgtgatgtgg gcactggaag ctgataccgt agaatacggt
    61381 gaatacctga ccggcgtgcg aattgaaggg ctcacctatt cactattctc atttacccgt
    61441 aaatgcggtc aggcaatcgg aggttcaatt cctgccttta ttttgggatt aagcggatat
    61501 atcgccaatc aggtgcaaac gccggaagtt attatgggca tccgcacatc aattgcctta
    61561 gtaccttgcg gatttatgct actggcattc gttattatct ggttttatcc gctcacggat
    61621 aaaaaattca aagaaatcgt ggttgaaatt gataatcgta aaaaagtgca gcagcaatta
    61681 atcagcgata tcactaatta atattcaata aaaataatca gaacatcaaa ggtgcaacta
    61741 tgagaaaaat agtggccatg gccgttattt gcctgacggc tgcctctggc cttacctctg
    61801 cttatgcggc gcaactggct gacgatgaag cgggactacg catcagactg aaaaacgaat
    61861 tgcgcagggc ggataagccc agtgctggcg cgggaagaga tatttacgca tgggtacagg
    61921 gaggattgct cgatttcaat agtggttatt attccaatat tattggcgtt gaaggcgggg
    61981 catattatgt ttataaatta ggtgctcgtg ctgatatgag tacccggtgg tatcttgatg
    62041 gtgataaaag ttttggcttt gccctggggg cagtaaaaat aaaacccagt gaaaatagcc
    62101 tgcttaaact aggtcgcttc gggacggatt atagttatgg tagcttacct tatcgtattc
    62161 cgttaatggc tggcagttcg caacgtacat taccgacagt ttctgaagga gcattaggtt
    62221 attgggcttt aacaccaaat attgatctgt ggggaatgtg gcgttcacga gtatttttat
    62281 ggactgattc aacaaccggt attcgtgatg aaggggtgta taacagccag acgggaaaat
    62341 acgataaaca tcgcgcacgt tcttttttag ccgccagttg gcatgatgat accagtcgct
    62401 attctctggg ggcatcggta cagaaagatg tttccaatca gatacaaagt attctcgaga
    62461 aaagcatacc gctcgacccg aattatacgt tgaaagggga gttgctcggc ttttacgcgc
    62521 agctcgaagg tttaagtcgt aataccagcc agcccaatga aacggcgttg gttagtggac
    62581 aattgacctg gaatgcgccg tggggaagcg tatttggcag tggtggttat ttgcgccatg
    62641 caatgaatgg tgccgtggtg gataccgaca ttggctatcc cttttcatta agtcttgatc
    62701 gtaaccgtga aggaatgcag tcctggcaat tgggcgtcaa ctatcgttta acgccgcaat
    62761 ttacgctgac atttgcaccg attgtgactc gcggctatga atccagtaaa cgagatgtgc
    62821 ggattgaagg cacgggtatc ttaggtggta tgaactatcg ggtcagcgaa gggccgttac
    62881 aagggatgaa tttctttctt gctgccgata aagggcggga aaagcgcgat ggcagtacgc
    62941 tgggcgatcg cctgaattac tgggatgtga aaatgagtat tcagtatgac tttatgctga
    63001 agtaaaaaat aacgccggag agaaaaatct ccggcgtttt gtctgtgaat tactgctgcg
    63061 gaatagcggg tacgaccgga acatttaatg ccggcattgc aaacatacca acgaaatctt
    63121 ccagtggcat tttctgcccg tttaacgtta tctgaccgtt agtatattgc aggctggtgg
    63181 tgatggtatt gtcctgcaag gtggtcagac ggaacatctg ccccattgct gatgcacctt
    63241 caacttgctg tttcgccagt tttttcgctt gatcttcctg ataaccttcc agcttcgcta
    63301 cctgagtcat aaactcagtt gccatatcca ccggaatggt cagtttcgca tccagagatt
    63361 taaccgaacg atctacttcc tgcgccagcg tttgcggcgc ttctttagtc gttgccggat
    63421 ctttcaggaa cagcgacaga ttcagcgcac tttcaccctg actgtttttc cagcttagcg
    63481 gcgcgatagt aatcaccgga tcgcctttca acatcagcgg cagggcgcta aagaaggctt
    63541 ccgtcacttt ctcctgataa agttcgggat tgttggcaat ttctggctgt gccagcagcg
    63601 cctgagtttg cgcgttatat tgctggctaa actgatgcca ggcttcacca tcaatctggc
    63661 cgacttttaa agtcagcttg ccgctgccca gatcctgatt ctgtaccttc aggctgttta
    63721 gcgagtaatc cagttggcta ttgatcgttt taccgtcatt gaccagatcc gatttaccgc
    63781 tgatctccat gccttccagc agtgccagtt ctttgccttc cactgaaatg gtcatttttt
    63841 ccagtgacag tttttggttt cctacgcgct caccaaaact tgccagcgtg ctggaaccgt
    63901 cggttttcag attattaaag gtcaactgca ctttctggtt gtattcgtta actgcgtcta
    63961 tccgaccact ttgcgcctcc ccggaaaggg agatggcttt gccgtctctg tcagcattta
    64021 actggaactc gccgccgcta aaggcgactt tttcatcctt ttgctcgtaa ttcagtggct
    64081 tgagcgaaat atcggaactg gaatcaccgc tgtaaccaat gcgcgagtta atctcaaaag
    64141 gcgtttcacc ttttgccata tcaaacagtg gtttgcttac ttcgttatta accagcgtgg
    64201 tttgaattga tgccatcgac gggatcaggt tcagtttttt aagctgggca agcgggaagg
    64261 gaccatgatc aaccgattcg ttgaagatga cgctctgacc gcttttaatc cacggatttt
    64321 ctttcccggc aatgggtttc accaacagtt gcaactggct gctgaatacg ccgcgatgat
    64381 agttttgata actcacttcc aggttggatt caggagctgt cagtttgagt tgcgcgttcg
    64441 cctgcgcgac catgtcttcg agatgggttt caatcttctt gcctgtatac catgcgccgc
    64501 ctgtccagac tacgcctagc gcaacaatga cgcctaccgc taccagcgat ttattcataa
    64561 tgattatcca taaaatgaaa tcaggcggac tggccgcctg aaggtgttat aagcctttaa
    64621 taagcttagc aagagatgtt aattttttca gtaagctctt acagcttgtt gtaaacacgc
    64681 gctaaacggc cgtggccttt gacagtcacc ggtgattcgt tggcggcaat aaacgctgat
    64741 tcacccggtt taagctgtaa ctgctgagaa cctttccaca acgttgcatc gccttcgacg
    64801 cagaacaaaa tggcggcact ctgctggcta atggtggttt ctttatcact aaggtcatgc
    64861 agcgagaagg caaaatcatc cactggaatc gggaagtcca gttctgcacc ttgtttcacc
    64921 ggctgggtca acaactggtt agccggtttg gcttcgaatt tcacattggc aaccagttcc
    64981 ggaatatcaa tgtacttagg cgtcagaccc gcacgcagca cgttatcgga gttcgccatc
    65041 acttccagcg ccacgccttg caggtaagcg tgcggtgttt cagcgaacag gaacatcgct
    65101 tcgccaggat tcaatttcac cacattcagt aacagcgggg agaacaggcc gctgtcttcc
    65161 gggtaaaatt cagaaattaa acgaatcgtt tgccacggtt caccctgctg actgtcgagc
    65221 gccgatttta aaatcgccag cgcgcgggat ttttcttcac cctgcatatt caacaggctg
    65281 gcgaacagtt cgcttaaacg ttcggcatta ggctgttgta aaaagtgagc aattgccgga
    65341 tgtgcacctg cgactggctg gagtatggag acaatctcgg aaaactcacg aaacgcgttc
    65401 atcgcaagga aaggcgtcag cgcaaaaacc agctccggct tgtggttagg atctttatag
    65461 ttacgctcgg cggcatccat cgggatacct gcggcatttt ctttggcaaa accgatttca
    65521 gaattgcgtt tgtttggatg aacctgaatg gagagcggct gtgctgcgca taatactttg
    65581 aacaggaaag gcagttcgcc aaagcgtttg gcaacggcct ctccgagcag agtcgattta
    65641 tcactctcaa tcacatcacg cagtgaaacg atatctccgg cggcattctg cactcgtgaa
    65701 ctgcttttcg gatgtgcgcc catccacagc tcggccatcg gctggctgga cggattttcc
    65761 ataccataaa gttcagtcaa cgccgttttg ctgccccagg catagttttg cactgagtta
    65821 atgagttttt gcatgatcaa tccctgtttt aatgtggaaa ttaatcccac tattaaagca
    65881 agaatcctac gggaagtaac ctggagccgc aaaaagtcgt actagtctca gtttttgtta
    65941 aaaaagtgtg taggatattg ttactcgctt ttaacagggc aacggaacac ccgcccagag
    66001 cataaccaaa ccaggcagta agtgagagaa caatgtcaaa caaacccttt cattatcagg
    66061 ctccttttcc actcaaaaaa gatgatactg agtattacct gctaaccagc gaacacgtta
    66121 gcgtatctga atttgaaggg caggagattt tgaaagtcgc acccgaagcg ttaactctgt
    66181 tggcgcgtca ggcgtttcat gatgcgtcgt ttatgctgcg tccggctcac caacaacagg
    66241 tggccgacat tctgcgtgac ccggaggcca gcgaaaatga taaatatgtg gcgctgcaat
    66301 tcctgcgtaa ctccgacatc gcggcgaaag gcgttctgcc aacctgtcag gataccggca
    66361 ctgcgattat tgttggtaaa aaagggcagc gtgtatggac cggtggctgt gatgaagcgg
    66421 cgctggcgcg cggtgtctat aacacttata tcgaagataa tttgcgctac tcgcaaaacg
    66481 cgccgctgga tatgtataaa gaggtgaata ccggcaccaa tctgccagcg cagatcgatc
    66541 tttatgccgt tgatggcgac gagtacaaat tcctctgtat cgccaaaggt ggtggttcgg
    66601 caaacaagac gtatctctat caggaaacca aagcgttact aacgccgggg aaactgaaaa
    66661 attacctggt tgagaagatg cgcacgctgg gtacggcggc ctgtcctccg tatcatattg
    66721 cgttcgtaat tggtggaact tctgcagaaa ctaaccttaa aacagtgaaa ctggcttccg
    66781 ctaaatacta tgacgaactg ccaacggaag ggaatgagca cggtcaggca ttccgcgatg
    66841 tggaactgga aaaagaattg ctgatcgaag cgcaaaatct cggtctgggt gcgcagtttg
    66901 gtggtaaata cttcgctcac gacatacgcg tgattcgcct gccacgtcac ggcgcatcct
    66961 gtccggttgg tatgggtgtt tcctgctctg ctgaccgtaa tatcaaagcg aagatcaacc
    67021 gtcaggggat ctggatcgaa aaactggaac ataatccagg taaatatatc cctgaagagc
    67081 tgcgtaaagc gggagaaggc gaagcggtgc gcgttgacct taaccgtccg atgaaagaga
    67141 tcctcgcaca gttgtcgcag tatcccgttt ctacacgctt atcgcttaac ggcacgatta
    67201 tcgtcggtcg tgatattgct cacgccaaac tgaaagagcg gatggataac ggtgaagggc
    67261 tgccgcagta cattaaagat catccgattt actacgcggg tccggccaaa acgccggaag
    67321 gttatgcctc cggttctctt ggcccaacga ccgccggacg gatggattct tatgtcgatc
    67381 aactgcaagc gcagggcgga agtatgatca tgctggcgaa aggcaaccgc agccagcagg
    67441 tgacggatgc ctgtaaaaaa cacggcggct tctaccttgg cagtatcggt ggtccggccg
    67501 ctgtattggc gcagggaagt attaagagcc tggaatgtgt tgaatatccg gaactgggaa
    67561 tggaagccat ctggaaaatt gaagtggaag atttcccggc gtttatcctt gtggatgata
    67621 aaggaaatga cttcttccag cagatacaac tcacacagtg cactcgctgt gtgaaataaa
    67681 cagagccgcc cttcggggcg gtttttttac atggcacgaa agaccaaaca tttgttatca
    67741 aatagtaaat aataagtgag ctaaaagttg cttaacgaaa gcaaaacaga aagaaaaaat
    67801 taaacaggtg aggagcaggc catgaataca gtacgcagcg aaaaagattc gatgggggcg
    67861 attgatgtcc cggcagataa gctgtggggc gcacaaactc aacgctcgct ggagcatttc
    67921 cgcatttcga cggagaaaat gcccacctca ctgattcatg cgctggcgct aaccaagcgc
    67981 gcagcggcaa aagttaatga agatttaggc ttgttgtctg aagagaaagc gagcgccatt
    68041 cggcaggcgg cggatgaagt actggcagga cagcatgacg acgaattccc gctggctatc
    68101 tggcagaccg gctccggcac gcaaagtaac atgaacatga acgaagtgct ggctaaccgg
    68161 gccagtgaat tactcggcgg cgtgcgcggg atggaacgta aagttcaccc taacgacgac
    68221 gtgaacaaaa gccaaagttc caacgatgtc tttccgacgg cgatgcacgt tgcggcgctg
    68281 ctggcgctgc gcaagcaact cattccgcag cttaaaaccc tgacacagac actgagtgaa
    68341 aaatcgcgtg catttgccga tatcgtcaaa atcggtcgaa cccacttgca ggacgccacg
    68401 ccgctaacac tagggcagga gatttccggc tgggtagcga tgctcgagca taatctcaaa
    68461 catatcgaat acagcctgcc tcacgtagcg gaactggctc ttggcggtac agcggtgggt
    68521 actggactaa atacccatcc ggagtatgcg cgtcgcgtag cagatgaact ggcagtcatt
    68581 acctgtgcac cgtttgttac cgcgccgaac aaatttgaag cgctggcgac ctgtgatgcc
    68641 ctggttcagg cgcacggcgc gttgaaaggg ttggctgtgt cactgatgaa aatcgccaat
    68701 gatgtccgct ggctggcctc tggcccgcgc tgcggaattg gtgaaatctc aatcccggaa
    68761 aatgagccgg gcagctcaat catgccgggg aaagtgaatc caacacagtg tgaggcatta
    68821 accatgctct gctgtcaggt gatggggaac gacgtggcga tcaacatggg gggcgcttcc
    68881 ggtaactttg aactgaacgt cttccgtcca atggtgatcc acaatttcct gcaatcggtg
    68941 cgcttgctgg cagatggcat ggaaagtttt aacaaacact gcgcagtggg tattgaaccg
    69001 aatcgtgagc gaatcaatca attactcaat gaatcgctga tgctggtgac tgcgcttaac
    69061 acccacattg gttatgacaa agccgcggag atcgccaaaa aagcgcataa agaagggctg
    69121 accttaaaag ctgcggccct tgcgctgggg tatcttagcg aagccgagtt tgacagctgg
    69181 gtacggccag aacagatggt cggcagtatg aaagccgggc gttaatctgc aacatacagg
    69241 tgcagccgtg gaatgatcaa acgaagcggc tgcgcctgtg gtttataacg gtgctgcaca
    69301 ttgtcggcat cgtaatttaa caactcacca acgtccggca cgcccgcgcc attatcccga
    69361 ttaatcagtg caatcagtgg tgtagggcag gcgtgttgga cttgtttttg atctcctttg
    69421 taccagacgc gggcaatcgg ctgcaccttc accggacgtt tgatttttaa cttcgcgttc
    69481 tgtggcaggg cagcgatatc ctgatactct cgctccagtt ttctttgcca ctcctcgcgc
    69541 gtccacggtg cgacactgcg tggtgatttc aggctttttt ccagctgtgc caggacttca
    69601 tcacgatgca aattcttaat gatatgttta ttagcccaac caaagcgtaa agtggcgggg
    69661 tcgtgcagaa cggtgagcgt gcggtaagca ttaagggtga tcagccccgg caaatgacga
    69721 tgcacccatt caaaacgtgc cgcggtgggg agttctgatt caaccgtgac gatatgctcg
    69781 aacgtggtct tgagtttatt gatgtgctga atatgactga ccaacgctgc ttgcgaaagg
    69841 ttatcgacct ggtaacacaa cacgccaggc agacggacag cggccttgct gctgcgattt
    69901 tcggactgtt gttgaataaa taaatggcgg aaatgacgca acgccagcga ctgcgcgtcg
    69961 ttgccgagat gttgttttac ctcaatacga ttaagcggat tatgctcatc ctcttttttt
    70021 acctccggca aagagaacac gcgggcaacc aatagcttgt gttgctcaag atgagcggca
    70081 aatgtagcca gctcttgttc catctggcga aaggtagtgt tgagtcggtc tacgagatcg
    70141 taacgcgcca taatattaac cactttagtt acaacatact tattttatag cacagtcgtg
    70201 gtgacttgac catcgtgcgc gtagtgtcag gcagaggtaa attgcgggat gttatgccat
    70261 aacggccagc taaacgagaa gcgagcacca cccagttcgc tggtgtcaca attaaccgta
    70321 ccgcccattg ccagtgctat agagtggaca attgccagcc ccagcccgca gccgccggtt
    70381 gagcgatccc ggctgggatc gaggcgaaca aaaggttcaa agatatgttc gcggttttct
    70441 ggggcaatcc ctgggccatc atcctcaaca attaatgtcg ctctattccc cgacagtagc
    70501 aggctggttt caaccgttga atggcagtac cgcagggcgt tattgagcaa attatccagc
    70561 acgcgctcca ttaagcgcat atccaacgcc gcataatggc cttgcacgag cgtttttatc
    70621 cgtaccgttt tatcgggcgt tactgcctga atatctgcca gatgcgttga cagccacaac
    70681 ggcaggtctg gttcgctaag atgaagctcg ttttgtgggc gatcgagtcg ggcataagtc
    70741 agcagctctt caattaaagc ttcaagttga ctgatatcac gattcaacgc ctgggattcg
    70801 gcggcgctca ggttatcgct catctccagt cgataacgca ggcgcactaa cggtgttcgc
    70861 agttcgtgag cgataccgtc aataagctgt tttttgctgg caatcaaggc gttgatattg
    70921 tccgccatct ggttaaatgc gataccaagt cgttcaaagc tcgaaccctc atcaaagtgg
    70981 atacgctcat tgagatgccc atcgccaaat cgttgcgccg ctgcttccag ttttaacata
    71041 tcctgccagt gcggacgcat ccagataaac accggaaagg cgagggaaat agcaataaaa
    71101 gcgatcaggg cgatatccag caatcgcatc tgatggagat aataaagata aggaacagga
    71161 ccaactgcca gcacgtagtg gctgcgcggg atacgctgca aaaacgtgta ctgatcgtcc
    71221 agggcgacaa tttcgccgcc acgcagtcgg tgcatggaaa tatcatcaag atggtattta
    71281 ctcagtggct cgacacgcag atcgaaagag agatttaaat ccatctcttt cagcgtctta
    71341 ccccagtcgt gtggggggat ctcacgcaat tcgctgcgca tcagatacag cgaactgttc
    71401 atcaaatcat ccagcgactg tttgcccgcg cgttcggcgg taaatttgta caccaaccca
    71461 accagcagag acatcacaag gaagcagaca aacaataaca ggtaaaactg gataaacagt
    71521 tttttcatcg cttattccca tgcatgaggc gcaaaaagat agcctttgtt acgcacagtt
    71581 ttaatgcgat aaggttctgc ggcgttatcg agcagttttt ttcttaaccg cgaaatagcc
    71641 acgtccacgc tacgatccag tccgtcataa ctgacgccgc gtaaattttt cagcaatgca
    71701 tcgcggtcca tgatttgccc ggcatgggta gctaattccc acaataattc gaaatcagct
    71761 gtcgagagcg agatttcagt gttagccagg gtgactacgc ggttgatggg atcgatggtc
    71821 aacgtgccga aatgcagggc tttgtaggga gtcagagacg tttcctgaag acctttggtc
    71881 agtgtggctt gctcattctg acgcaaatgc aaacgtaaac gcgctagcaa aacagcaggg
    71941 ggcgtcgttt tgagaatata gtcgcaggca cccatttcca gtgccaggat gtggttcata
    72001 tcgctatcga gagaggttag aagaacaatc ggtccagacc actttgcgcg taaatcacga
    72061 caaatggtca tgccgtcctt gcctggtagc atgatgtcga gtaacaccaa atccggactt
    72121 tctcgcaaaa tggtttcttc ggcctggtcg ccgcgcggtt ctacggtaac ctgcatatca
    72181 tgttttgcca ggtacgcggc aatcagtgaa ccgacttccg catcatcttc cacaaatacg
    72241 atagtgttca taacattcac ggtagatata aaaacgtcac aatacaccgc gctgttttta
    72301 cttactatca atcgtttcta aacaagtttt catcaggtat tctactgatg acttgttatg
    72361 gaagtgttaa ggtaaaaaga tggggctggt aatcaaagcc gcgctcggtg cgctggtagt
    72421 gctgttgatt ggtgttttag caaaaacgaa aaattattat atcgccgggc tgattccact
    72481 tttcccgacc tttgcgctta tcgcgcatta tattgttgcc agcgaacgcg gcattgaagc
    72541 cttacgcgca accatcattt ttagtatgtg gtcgattatt ccctattttg tttacctggt
    72601 gtcgctgtgg tatttcaccg gaatgatgcg cttgcccgcc gcttttgttg gttctgttgc
    72661 gtgttggggg ataagcgcat gggtgttgat tatatgctgg ataaagctgc attaacgcag
    72721 atgacgaccg ccatcaagtg ggaaactgcg tccggtgaca aagcagctgg taagtaagta
    72781 atcgaccagg tcgatcactt ctttctcgcc aggcgcggtt ttcatcagtg atttattcag
    72841 cgcctgttgt cgatattcgg catcatcatg ttcattaaac aggatcagcg atggcgcaat
    72901 agaattcact ttcacttccg gtgccagctt gcgggcaaac gagcgggtca tattatccag
    72961 tgccgctttg cttgcagcat acgcaacatg tttgtcgcta ccgcgctcca ccacataatc
    73021 ggtaaagtga atgatatcgc tggcggcgtg tccgtgccca cgcagtaatc tttccagcgc
    73081 atggttgagc aggtatgggg tattaacgtg gatctgcatc atgcaagcca gtacgtcggc
    73141 cagtggtgta cctggttttt ccgccatcca cgcactggcg ttatgcaaaa tagcacgcag
    73201 accatgggtg ctttttagta cttcatcggc aaacgccatc acaccgtcgt tggtcgaaaa
    73261 atcagcctga atacactgcg cacctgcctt aatcagtcca tcaatggctg gatagtgcgt
    73321 ccgatagctg acaatcaccg gttgcttttg attaatgaaa tgccaggcga gggcgaggcc
    73381 gatgcgacga cctccgccag taattaatat tggcaagggc tgggctttac ccatcgttat
    73441 ctcctttgct atccaacgat ggtatgaacg ttatcccacc agcatccagg tcgccggaac
    73501 tgaggcaatt aacagcatgc caatcagcac catttcctga cggttcagta cattatcatg
    73561 tgtatgcgtt ttgcgcgcat acaggaaaac cagcagcccc ggcgcataca gaacaacgga
    73621 taacagcaag tgcattggac cagaggcata caataaccat aagccataaa tgcaggcacc
    73681 gacacctacc gctttatgca gtggacgggt tgcgattttc agcaagaacg cgccgaccag
    73741 gaaatagggc accagaatca tttctgaggc gatggtcagc aacgtgttgt aatcggaacc
    73801 ggtcagccag atgagtacca gacaaatttg cacgcagatg ttggtcaacc acagcgaggc
    73861 ggatggcgca gcttgtgcgt tctggcgcgc aaaaatgcgt gggaatgctt tatgagtggc
    73921 agccaggaac ggaacttctg ccgccatgat ggtccagctt aagtacgcgc cgcaaacgga
    73981 aacgatcaaa ccggcagcga tgatgatttc gccccatggt cccatcattt ccaccatcag
    74041 accggccatt gacgggttac gaatttcagc cagttcagga cgtgccacca cgcccagcga
    74101 aagcagcgtt accagcaagt aaacgcccag agcggagaga accgccagca gtgtcgcttt
    74161 gccaacatca cgtttattac gcgcacgcgc agaaacaacc acggcacctt ccacaccaat
    74221 gaaaacccac agggtgatca gcatggtgtt tttcacctgt tcccacacgg gtacgccaag
    74281 tgcaagtccg gtgaagtcga gcttgaaggt atccagtttg aacatcatca tcgccagcac
    74341 aacaaacaga cccagcggca acaattttgc cagtgtcgcc accaggttaa tgctggcagc
    74401 ggtttgcacc ccgcgcagga tcaaaaagtg aacaatccac aataaagccg atgcaccgac
    74461 tatcgattgc caggtattac catcgccgaa caggcgcaat tccggcgtgt ccgtaaaaaa
    74521 gcttaacgcg gaaaaaacga tcaccagata ggagacgttg gcgatgactg cgcacagcca
    74581 gtatccccat gcggaacaaa agccgattag ctcgccaaac ccttcgcggg cataggtaaa
    74641 gataccgccg tcaagttcgg gacgaatgcg cgtgaggatc agcatggcaa aggccagcaa
    74701 taaaatgcca gcgccagtaa taccccagcc gatgagcagt gctgccgggc tggcaactgc
    74761 cgccatattt tgcggcagac tgaaaacacc cgcgcccagc attgagctta ataccagcgc
    74821 ggtgagtgcg ctcagtccca gtttcttttc catcgatatc cagtgattaa aacgtgataa
    74881 gaagaataaa tattagggca aaacgcataa aaatggagtt tgccactgaa gcgcgggatt
    74941 ttacgaagag atgtgtctgc atgcaatggt cgcggggaaa tttgtgacta aaaatgcgaa
    75001 aggcggcatc aagccgcctt ataggagtaa ctgaatagtt atttatacag atctgcgctg
    75061 acggtcaggt tattaccacg ttcctgccac tggcgggtga tgtggtaata cttagcacct
    75121 ttcttcgcgg cacgtttcgc aacctgatag gagacttcgg tcatgttgcc gtagttgcca
    75181 gagaatttga tgctgtcgaa cgggaccatc atcgctgctg tcgctttgtt cagttcttcg
    75241 actttagtgc cgtcaggaag cgtgacagtg taacgtccgc cttttgatga ctgggtttca
    75301 aagaaacgac cgacttcaga acttggtgat gcggtagtcg caacacctgg gatctcaact
    75361 ttcttcgcgg cttcgccacc ggcagccaga gctgcacgtc ctgcttcgga atctgccggg
    75421 atcacatccg ggctctggac gatacgtttc ttagcatctt ttttatagat gaatgcagta
    75481 atacgctggt tgccgccctg gttggcatcg atttgacgaa caatgtagaa agagtaggca
    75541 cctttcgctt ttgctgcttt ggtgatggca tcattgactt ctggctggct acgatagaag
    75601 ccctggacgg tgaccgtgtc aaacggttca atcagaacag cctgatcttt cggcagttcg
    75661 acaacaccgt taattacgcg attacttgtt tcttctgctt tttcagcatc ggctttatag
    75721 aggtcagcga ccacacgcca gttaccgctg ttaccaaaat cagaagtgtc gacaacataa
    75781 aaagaggcgg caccttcttt atctgcgcga cgagaaacgg ctttcaccgc ttcgccaata
    75841 gcattaaaac gaccggtaac cactacacgg tcaaaaggtt taaccgctgc cgcttgctcc
    75901 ggtgtcagtt ctgttgctgc gttaacggag aatgccgtag cagaaagcag tgccgacgcc
    75961 aggagggtgt tcttaagctt cataaaaata atccttcgcc ttgcgcaaac caggtactgg
    76021 tattgttatt aacgagaaac gtggctgatt attgcattta aacggtgtaa ctgtctgcgt
    76081 catttttcat atcacattcc ttaagccaat tttaatcctg ctcaaatgac cgtctatgct
    76141 taaaaaacag ccgtatcagc atcattacta ctgaagcaac tgaattgtat aagttaattt
    76201 aatgttaagt agtgattcgt gccggggcga tgtctcgttt tacccgaccg tcgaagacaa
    76261 ttatcagtct ttatccggcg ttctaaggtg tttatcccac tatcacggct gaatcgttaa
    76321 tattttgcga gttcacgccg aaatactgat ttttggcgct agatcacagg cataattttc
    76381 agtacgttat agggcgtttg ttactaattt attttaacgg agtaacattt agctcgtaca
    76441 tgagcggctt gtgtggctcc tgacacaggc aaaccatcat caataaaacc gatggaaggg
    76501 aatatcatgc gaattggcat accaagagaa cggttaacca atgaaacccg tgttgcagca
    76561 acgccaaaaa cagtggaaca gctgctgaaa ctgggtttta ccgtcgcggt agagagcggc
    76621 gcgggtcaac tggcaagttt tgacgataaa gcgtttgtgc aagcgggcgc tgaaattgta
    76681 gaagggaata gcgtctggca gtcagagatc attctgaagg tcaatgcgcc gttagatgat
    76741 gaaattgcgt tactgaatcc tgggacaacg ctggtgagtt ttatctggcc tgcgcagaat
    76801 ccggaattaa tgcaaaaact tgcggaacgt aacgtgaccg tgatggcgat ggattctgtg
    76861 ccgcgtatct cacgcgcaca atcgctggac gcactaagct cgatggcgaa catcgccggt
    76921 tatcgcgcca ttgttgaagc ggcacatgaa tttgggcgct tctttaccgg gcaaattact
    76981 gcggccggga aagtgccacc ggcaaaagtg atggtgattg gtgcgggtgt tgcaggtctg
    77041 gccgccattg gcgcagcaaa cagtctcggc gcgattgtgc gtgcattcga cacccgcccg
    77101 gaagtgaaag aacaagttca aagtatgggc gcggaattcc tcgagctgga ttttaaagag
    77161 gaagcgggca gcggcgatgg ctatgccaaa gtgatgtcgg acgcgttcat caaagcggaa
    77221 atggaactct ttgccgccca ggcaaaagag gtcgatatca ttgtcaccac cgcgcttatt
    77281 ccaggcaaac cagcgccgaa gctaattacc cgtgaaatgg ttgactccat gaaggcgggc
    77341 agtgtgattg tcgacctggc agcccaaaac ggtggcaact gtgaatacac cgtgccgggt
    77401 gaaatcttca ctacggaaaa tggtgtcaaa gtgattggtt ataccgatct tccgggccgt
    77461 ctgccgacgc aatcctcaca gctttacggt actaacctcg ttaatctgct gaaactgttg
    77521 tgcaaagaga aagacggcaa tatcactgtt gattttgatg atgtggtgat tcgcggcgtg
    77581 accgtgatcc gtgcgggcga aattacctgg ccggcaccgc cgattcaggt atcagctcag
    77641 ccgcaggcgg cacaaaaagc ggcaccggaa gtgaaaactg aggaaaaatg tgcctgctca
    77701 ccgtggcgta aatacgcatt gatggcgctg gcaatcatcc ttttcggttg gctggcaagc
    77761 gttgcgccga aagagtttct tgggcacttc accgttttcg cgctggcctg cgttgtcggt
    77821 tattacgtgg tgtggaatgt atcgcatgcg ctgcatacac cgttgatgtc ggtcaccaac
    77881 gcgatttcag ggattattgt tgtcggagca ctgttgcaga ttggccaggg cggctgggtt
    77941 agcttcctta gttttatcgc ggtgcttata gccagcatta atattttcgg tggcttcacc
    78001 gtgactcagc gcatgctgaa aatgttccgc aaaaattaag gggtaacata tgtctggagg
    78061 attagttaca gctgcataca ttgttgccgc gatcctgttt atcttcagtc tggccggtct
    78121 ttcgaaacat gaaacgtctc gccagggtaa caacttcggt atcgccggga tggcgattgc
    78181 gttaatcgca accatttttg gaccggatac gggtaatgtt ggctggatct tgctggcgat
    78241 ggtcattggt ggggcaattg gtatccgtct ggcgaagaaa gttgaaatga ccgaaatgcc
    78301 agaactggtg gcgatcctgc atagcttcgt gggtctggcg gcagtgctgg ttggctttaa
    78361 cagttatctg catcatgacg cgggaatggc accgattctg gtcaatattc acctgacgga
    78421 agtgttcctc ggtatcttca tcggggcggt aacgttcacg ggttcggtgg tggcgttcgg
    78481 caaactgtgt ggcaagattt cgtctaaacc gttgatgctg ccaaaccgtc acaaaatgaa
    78541 cctggcggct ctggtcgttt ccttcctgct gctgattgta tttgttcgca cggatagcgt
    78601 cggcctgcaa gtgctggcat tgctgataat gaccgccatt gcgctggtat tcggctggca
    78661 tttagtcgcc tccatcggtg gtgcagatat gccagtggtg gtgtcgatgc tgaactcgta
    78721 ctccggctgg gcggctgcgg ctgcgggctt tatgctcagc aacgacctgc tgattgtgac
    78781 cggtgcgctg gtcggttctt cgggcgctat cctttcttac attatgtgta aggcgatgaa
    78841 ccgttccttt atcagcgtta ttgcgggtgg tttcggcacc gacggctctt ctactggcga
    78901 tgatcaggaa gtgggcgagc accgcgaaat caccgcagaa gagacagcgg aactgctgaa
    78961 aaactcccat tcagtgatca ttactccggg gtacggcatg gcagtcgcgc aggcgcaata
    79021 tcctgtcgct gaaattaccg agaaactgcg cgctcgtggt atcaacgtgc gtttcggtat
    79081 ccacccggtt gcggggcgtt tgcctggaca tatgaacgta ttgctggctg aagcaaaagt
    79141 accgtatgac atcgtgctgg aaatggacga gatcaacgat gactttgctg ataccgatac
    79201 cgtactggtg attggtgcta acgatacggt taacccggcg gcgcaggatg atccgaagag
    79261 tcctattgct ggtatgcctg tgctggaagt gtggaaagcg cagaacgtga ttgtctttaa
    79321 acgttcgatg aacactggct atgctggtgt gcaaaacccg ctgttcttca aggaaaacac
    79381 ccacatgctg tttggtgacg ccaaagccag cgtggatgca atcctgaaag ctctgtaacc
    79441 ctgacggcct ctgcggaggc cgtcactctt tattgagatc gcttaacaga acggcgatgc
    79501 tttgacctcc cgctgtttgt tcaagcgcaa ttttgacaat aattgttaac ggcacggaaa
    79561 gcagcatacc caccggtcct aacaaccatc cccaaaaaat caacgacaaa aataccacca
    79621 atgtggaaag ccccagccca cgtcccatga tgcgcggctc cagaatattg ccgaagacca
    79681 gattaatcag cagatatccc gccagcacca gcaacgcttc gtagaagcca ttaaacacca
    79741 gtacctgagc gatagggggg attgccgcga gaactgaacc aatattaggg atgtaattaa
    79801 gcgcaaaggc cagcaatccc cagacaaaag cgaagcgaac atcgagtgcg gcgagcatcg
    79861 cccaggcgac caggccggtg atgatgctga tggctgtttt cagcaccaga taatgagaaa
    79921 cactgtcaat tgcacgttga atcgccgcca tcccttcaac cggacgcgcc atcatttgct
    79981 gaaattttcc gggcaattgt ggcacttcga gcagcataaa cagcaccgtc agcagcaata
    80041 aaaatattga tgacatggca ttagataact gcgtcaataa gttggtgagc aacgtcatcg
    80101 ccgcgttcgg atcaatgtaa tgcgccagct ggtcaactga gacgtcaatc cctacgcgtt
    80161 gcaacaacgg ctcaagagct tgcagcggcg tcataataga gttgcgatat tgcggtaacg
    80221 tccgcgtcaa ctcgttgagc gcggaaccca gataagctaa tagcaacacc atcgccatca
    80281 cgatgatggt catcaaaatc gacaccgcca gtacacgcgg cactcgccag cggaccatgt
    80341 gttgcaccag cgggttaaga ataacagcat tttatcattt agtgtttttt gctgtttcat
    80401 aatgtttcag gctgtgcatt atgtttttga ttatcaacag atttgtgatt ttccttgttc
    80461 cagcttgttt cacgttgtag ccctttgtgc aacgatcggt gtagtcactg gtgtagtcac
    80521 tttttcgtaa agagggggta aaacgtgagc agagcaatga acaaactgag cgataccttg
    80581 ctaaggaaac ttcatggtaa gccagcggag aaaaacacgt tttatagtga tggcggaaac
    80641 ctgagcgtga aatatttaac atcagggaaa ttgacctggt atttcacata cagggccgga
    80701 acgggaaggg agacacgacc cgaacgcatt aagctgggaa gttatcctga tctgagcctg
    80761 aaagcagcca gggaaaaagc cgcacagtgt cgcacatggc tggctgaggg gaaaaatcca
    80821 cgtcatgaga tgaattacac cgtacaggaa gcgttaaagc ccgtaacggt tggcgatgcg
    80881 ctcacctact ggcttgagta ttacgtaaag gaaaaccgcg tggattatat cgccctgaaa
    80941 aggcgactta ataatcacgt aatacagcag attggtgcta tgccgctgga taaatgcgag
    81001 ctacggcact ggctggcctg ttttgaccag gtggcaaagc gaacgcctgt tactgccgga
    81061 ttcgtgctac aggcgtgcaa acaggcgctt aagttctgcc gtaggcggcg ctatgcaatc
    81121 agcaacgttc tggacgatct gaacgtggcg gacgttggga aaaaaccgga tataagcgaa
    81181 cgtgtcttaa gtaacaaaga acttggcgaa ttattgcagg cactggacaa aaaaatattt
    81241 tccccttact acgtcgcgtt aatccgcctc ctgattgtct tcggagccag gacggtagaa
    81301 ctgaggctat cggagatcgg ggagtgggat tttacggaaa tgctatggac cgttccgaag
    81361 gagcacagca aaacgaaggt cgcaatattc cggcccatac cggaagcgat cctgccgttc
    81421 gtcacgcagc tggtggagca gaacaggcac acgggcttat tgctggggga agtgaaacag
    81481 gaggccagcg tatcgcagta tggaagatta gcgcacagga ggcttaatca tcctcactgg
    81541 tcactgcatg acatccggcg cacctttaca accatgctga acgatttagg cgttgatccg
    81601 catgtcgtgg agcagcttac aggccaccag atgccaggaa tgcagcgagt ttataatcat
    81661 tcccgttatc tggatgctaa acgcaatgtg ctggatatgt ggatggagcg gttagggata
    81721 ctggcgggaa cacatgaaaa cgtaaccacg ctaccagtag ccagaagaaa ataatttttt
    81781 tcgtattttt tcagtatgcg catactggat atgcgaacag atacaacgtg caacaatgag
    81841 caacaatgcg gaacaactac gaacaagagg cgaaaaagtg tacagattta taagtagctg
    81901 atttttaatg tgttactggt tttttataca ctagcgaatc actctttaaa tagcgagaaa
    81961 aaaggcatga aacaatattt tatgcctttg tattaacatg aatttaaatg attttatcct
    82021 gattattcct ctttctgtaa accatacggc ctcaacattt gccaactctg aatcatcctg
    82081 acacaatcag acacatccca acgcaataga acacgaaata actctttaag aaacgaaagg
    82141 gggcaatagt gttaagcact gatcggttta tacgtgaaaa agaatgcgaa aagctaacag
    82201 gccttagccg ctcatgccgc taccgcctgg aaaaggccgg acaattccca tcacgtcgta
    82261 aacttggcgg tcgttccgtt ggctggtctt tatccgaggt tctggcctgg aaggatagct
    82321 gcaaggcagt tcattaatca cgctggcggc acgcagccgc cacacatcaa tcatctgaac
    82381 acagagccat aaccatgaag attgaatata cgccagaacg tgggcgggga ttcgttcgcc
    82441 ctggtgaaac tgaaaaacaa caaaattggg gtttttcagg tataaaaaaa gcggccccga
    82501 aatggagccg cctttctgaa cagataaccc gctgcgccgt ttgtgt
//
LOCUS       DAZGOB010000004        82004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGOB010000004 DAZGOB010000000
VERSION     DAZGOB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 82004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.004"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..596)
                     /locus_tag="ACHLIF_000243"
     CDS             complement(<1..596)
                     /locus_tag="ACHLIF_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO9604319.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHLIF_000244"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHLIF_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO9604320.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHLIF_000245"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHLIF_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO9604321.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHLIF_000246"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHLIF_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO9604322.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHLIF_000247"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHLIF_000247"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO9604323.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHLIF_000248"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHLIF_000248"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO9604324.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHLIF_000249"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHLIF_000249"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO9604325.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHLIF_000250"
     CDS             complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHLIF_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9604326.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHLIF_000251"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHLIF_000251"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9604327.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /gene="slyX"
                     /locus_tag="ACHLIF_000252"
     CDS             6907..7125
                     /gene="slyX"
                     /locus_tag="ACHLIF_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO9604328.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHLIF_000253"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHLIF_000253"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO9604329.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHLIF_000254"
     CDS             complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHLIF_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO9604330.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHLIF_000255"
     CDS             complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHLIF_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO9604331.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHLIF_000256"
     CDS             complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHLIF_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO9604332.1"
                     /translation="MSQPAKVLLLFAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10556..12469
                     /gene="yheS"
                     /locus_tag="ACHLIF_000257"
     CDS             10556..12469
                     /gene="yheS"
                     /locus_tag="ACHLIF_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9604333.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12469..13491
                     /gene="yheT"
                     /locus_tag="ACHLIF_000258"
     CDS             12469..13491
                     /gene="yheT"
                     /locus_tag="ACHLIF_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO9604334.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13485..13703
                     /gene="yheU"
                     /locus_tag="ACHLIF_000259"
     CDS             13485..13703
                     /gene="yheU"
                     /locus_tag="ACHLIF_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO9604335.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13757..14626
                     /gene="prkB"
                     /locus_tag="ACHLIF_000260"
     CDS             13757..14626
                     /gene="prkB"
                     /locus_tag="ACHLIF_000260"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO9604336.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHLIF_000261"
     CDS             complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHLIF_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO9604337.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15387..16019
                     /gene="crp"
                     /locus_tag="ACHLIF_000262"
     CDS             15387..16019
                     /gene="crp"
                     /locus_tag="ACHLIF_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO9604338.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHLIF_000263"
     CDS             16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHLIF_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO9604339.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHLIF_000264"
                     /gene_synonym="dapC"
     CDS             complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHLIF_000264"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO9604340.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHLIF_000265"
     CDS             complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHLIF_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO9604341.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHLIF_000266"
     CDS             complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHLIF_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO9604342.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHLIF_000267"
     CDS             complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHLIF_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO9604343.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHLIF_000268"
                     /gene_synonym="rotA"
     CDS             complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHLIF_000268"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO9604344.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHLIF_000269"
     CDS             21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHLIF_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO9604345.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23278..25821
                     /gene="nirB"
                     /locus_tag="ACHLIF_000270"
     CDS             23278..25821
                     /gene="nirB"
                     /locus_tag="ACHLIF_000270"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO9604346.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25818..26144
                     /gene="nirD"
                     /locus_tag="ACHLIF_000271"
     CDS             25818..26144
                     /gene="nirD"
                     /locus_tag="ACHLIF_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO9604347.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26270..27076
                     /gene="nirC"
                     /locus_tag="ACHLIF_000272"
     CDS             26270..27076
                     /gene="nirC"
                     /locus_tag="ACHLIF_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO9604348.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLSNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27095..28468
                     /gene="cysG"
                     /locus_tag="ACHLIF_000273"
     CDS             27095..28468
                     /gene="cysG"
                     /locus_tag="ACHLIF_000273"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO9604349.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28715..28882
                     /gene="yhfL"
                     /locus_tag="ACHLIF_000274"
     CDS             28715..28882
                     /gene="yhfL"
                     /locus_tag="ACHLIF_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO9604350.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            29177..30514
                     /gene="frlA"
                     /locus_tag="ACHLIF_000275"
     CDS             29177..30514
                     /gene="frlA"
                     /locus_tag="ACHLIF_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO9604351.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30535..31557
                     /gene="frlB"
                     /locus_tag="ACHLIF_000276"
     CDS             30535..31557
                     /gene="frlB"
                     /locus_tag="ACHLIF_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO9604352.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31607..32437
                     /gene="frlC"
                     /locus_tag="ACHLIF_000277"
     CDS             31607..32437
                     /gene="frlC"
                     /locus_tag="ACHLIF_000277"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO9604353.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32434..33219
                     /gene="frlD"
                     /locus_tag="ACHLIF_000278"
     CDS             32434..33219
                     /gene="frlD"
                     /locus_tag="ACHLIF_000278"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO9604354.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33319..34050
                     /gene="frlR"
                     /locus_tag="ACHLIF_000279"
                     /gene_synonym="yhfR"
     CDS             33319..34050
                     /gene="frlR"
                     /locus_tag="ACHLIF_000279"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO9604355.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34202..35287)
                     /gene="yhfS"
                     /locus_tag="ACHLIF_000280"
     CDS             complement(34202..35287)
                     /gene="yhfS"
                     /locus_tag="ACHLIF_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO9604356.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35299..36603)
                     /gene="yhfT"
                     /locus_tag="ACHLIF_000281"
     CDS             complement(35299..36603)
                     /gene="yhfT"
                     /locus_tag="ACHLIF_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO9604357.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36615..36968)
                     /gene="yhfU"
                     /locus_tag="ACHLIF_000282"
     CDS             complement(36615..36968)
                     /gene="yhfU"
                     /locus_tag="ACHLIF_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO9604358.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36979..37857)
                     /gene="php"
                     /locus_tag="ACHLIF_000283"
     CDS             complement(36979..37857)
                     /gene="php"
                     /locus_tag="ACHLIF_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO9604359.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37854..39080)
                     /gene="yhfW"
                     /locus_tag="ACHLIF_000284"
     CDS             complement(37854..39080)
                     /gene="yhfW"
                     /locus_tag="ACHLIF_000284"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO9604360.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(39080..40243)
                     /gene="yhfX"
                     /locus_tag="ACHLIF_000285"
     CDS             complement(39080..40243)
                     /gene="yhfX"
                     /locus_tag="ACHLIF_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO9604361.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPMLAEYGVTHAEPGHALTGTIPANQQGDQPEYIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(40327..40689)
                     /gene="yhfY"
                     /locus_tag="ACHLIF_000286"
     CDS             complement(40327..40689)
                     /gene="yhfY"
                     /locus_tag="ACHLIF_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO9604362.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40706..41611)
                     /gene="yhfZ"
                     /locus_tag="ACHLIF_000287"
     CDS             complement(40706..41611)
                     /gene="yhfZ"
                     /locus_tag="ACHLIF_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO9604363.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41880..42884)
                     /gene="trpS"
                     /locus_tag="ACHLIF_000288"
     CDS             complement(41880..42884)
                     /gene="trpS"
                     /locus_tag="ACHLIF_000288"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO9604364.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42877..43635)
                     /gene="gph"
                     /locus_tag="ACHLIF_000289"
     CDS             complement(42877..43635)
                     /gene="gph"
                     /locus_tag="ACHLIF_000289"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO9604365.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43628..44305)
                     /gene="rpe"
                     /locus_tag="ACHLIF_000290"
     CDS             complement(43628..44305)
                     /gene="rpe"
                     /locus_tag="ACHLIF_000290"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO9604366.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKLSHE"
     gene            complement(44323..45159)
                     /gene="dam"
                     /locus_tag="ACHLIF_000291"
     CDS             complement(44323..45159)
                     /gene="dam"
                     /locus_tag="ACHLIF_000291"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO9604367.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45266..46552)
                     /gene="damX"
                     /locus_tag="ACHLIF_000292"
     CDS             complement(45266..46552)
                     /gene="damX"
                     /locus_tag="ACHLIF_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO9604368.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46644..47732)
                     /gene="aroB"
                     /locus_tag="ACHLIF_000293"
     CDS             complement(46644..47732)
                     /gene="aroB"
                     /locus_tag="ACHLIF_000293"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO9604369.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADSQSA"
     gene            complement(47789..48310)
                     /gene="aroK"
                     /locus_tag="ACHLIF_000294"
     CDS             complement(47789..48310)
                     /gene="aroK"
                     /locus_tag="ACHLIF_000294"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO9604370.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48711..49949)
                     /gene="hofQ"
                     /locus_tag="ACHLIF_000295"
     CDS             complement(48711..49949)
                     /gene="hofQ"
                     /locus_tag="ACHLIF_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO9604371.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49861..50265)
                     /gene="hofP"
                     /locus_tag="ACHLIF_000296"
     CDS             complement(49861..50265)
                     /gene="hofP"
                     /locus_tag="ACHLIF_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO9604372.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(50255..50695)
                     /gene="hofO"
                     /locus_tag="ACHLIF_000297"
     CDS             complement(50255..50695)
                     /gene="hofO"
                     /locus_tag="ACHLIF_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO9604373.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50679..51218)
                     /gene="hofN"
                     /locus_tag="ACHLIF_000298"
     CDS             complement(50679..51218)
                     /gene="hofN"
                     /locus_tag="ACHLIF_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO9604374.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(51218..51997)
                     /gene="hofM"
                     /locus_tag="ACHLIF_000299"
     CDS             complement(51218..51997)
                     /gene="hofM"
                     /locus_tag="ACHLIF_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO9604375.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            52117..54669
                     /gene="mrcA"
                     /locus_tag="ACHLIF_000300"
     CDS             52117..54669
                     /gene="mrcA"
                     /locus_tag="ACHLIF_000300"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO9604376.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54835..55395)
                     /gene="nudE"
                     /locus_tag="ACHLIF_000301"
     CDS             complement(54835..55395)
                     /gene="nudE"
                     /locus_tag="ACHLIF_000301"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO9604377.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55715..57850
                     /gene="igaA"
                     /locus_tag="ACHLIF_000302"
     CDS             55715..57850
                     /gene="igaA"
                     /locus_tag="ACHLIF_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO9604378.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWVPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57915..58583
                     /gene="yrfG"
                     /locus_tag="ACHLIF_000303"
     CDS             57915..58583
                     /gene="yrfG"
                     /locus_tag="ACHLIF_000303"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO9604379.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58594..58995
                     /gene="hslR"
                     /locus_tag="ACHLIF_000304"
     CDS             58594..58995
                     /gene="hslR"
                     /locus_tag="ACHLIF_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO9604380.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            59020..59898
                     /gene="hslO"
                     /locus_tag="ACHLIF_000305"
     CDS             59020..59898
                     /gene="hslO"
                     /locus_tag="ACHLIF_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO9604381.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(60033..61757)
                     /gene="yhgE"
                     /locus_tag="ACHLIF_000306"
     CDS             complement(60033..61757)
                     /gene="yhgE"
                     /locus_tag="ACHLIF_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO9604382.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYKSVWHNVLILLVIFLANGLTWVVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            62136..63758
                     /gene="pckA"
                     /locus_tag="ACHLIF_000307"
     CDS             62136..63758
                     /gene="pckA"
                     /locus_tag="ACHLIF_000307"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO9604383.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKELGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63834..65186)
                     /gene="envZ"
                     /locus_tag="ACHLIF_000308"
     CDS             complement(63834..65186)
                     /gene="envZ"
                     /locus_tag="ACHLIF_000308"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO9604384.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIDVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(65183..65902)
                     /gene="ompR"
                     /locus_tag="ACHLIF_000309"
     CDS             complement(65183..65902)
                     /gene="ompR"
                     /locus_tag="ACHLIF_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO9604385.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            66130..66606
                     /gene="greB"
                     /locus_tag="ACHLIF_000310"
     CDS             66130..66606
                     /gene="greB"
                     /locus_tag="ACHLIF_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO9604386.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66703..69024
                     /gene="yhgF"
                     /locus_tag="ACHLIF_000311"
     CDS             66703..69024
                     /gene="yhgF"
                     /locus_tag="ACHLIF_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO9604387.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVMSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69462..69689
                     /gene="feoA"
                     /locus_tag="ACHLIF_000312"
     CDS             69462..69689
                     /gene="feoA"
                     /locus_tag="ACHLIF_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO9604388.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69706..72027
                     /gene="feoB"
                     /locus_tag="ACHLIF_000313"
     CDS             69706..72027
                     /gene="feoB"
                     /locus_tag="ACHLIF_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO9604389.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            72027..72263
                     /gene="feoC"
                     /locus_tag="ACHLIF_000314"
     CDS             72027..72263
                     /gene="feoC"
                     /locus_tag="ACHLIF_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO9604390.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72466..73344
                     /gene="rpnA"
                     /locus_tag="ACHLIF_000315"
     CDS             72466..73344
                     /gene="rpnA"
                     /locus_tag="ACHLIF_000315"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO9604391.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(73373..74143)
                     /gene="bioH"
                     /locus_tag="ACHLIF_000316"
     CDS             complement(73373..74143)
                     /gene="bioH"
                     /locus_tag="ACHLIF_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO9604392.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            74181..74864
                     /gene="gntX"
                     /locus_tag="ACHLIF_000317"
     CDS             74181..74864
                     /gene="gntX"
                     /locus_tag="ACHLIF_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO9604393.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            74923..75498
                     /gene="nfuA"
                     /locus_tag="ACHLIF_000318"
     CDS             74923..75498
                     /gene="nfuA"
                     /locus_tag="ACHLIF_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO9604394.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            75859..77175
                     /gene="gntT"
                     /locus_tag="ACHLIF_000319"
     CDS             75859..77175
                     /gene="gntT"
                     /locus_tag="ACHLIF_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO9604395.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77220..79304)
                     /gene="malQ"
                     /locus_tag="ACHLIF_000320"
     CDS             complement(77220..79304)
                     /gene="malQ"
                     /locus_tag="ACHLIF_000320"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO9604396.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79314..81707)
                     /gene="malP"
                     /locus_tag="ACHLIF_000321"
     CDS             complement(79314..81707)
                     /gene="malP"
                     /locus_tag="ACHLIF_000321"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO9604397.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKMIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19565 a        22383 c        20727 g        19329 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat aatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga aaacggctcg tcatcacttg cgtgacgctc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatattac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     4021 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4141 tgcaataata gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagca ataaagaaga cggccagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc acttgccggt gaggtcaatt ggttagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactca tggccttcta ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc tggaaatcac cgcttttgaa ctggcggatg tagagataga
     5041 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcgtct ttaatatcga
     5101 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5161 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5221 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc
     5281 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5341 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5461 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5521 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt
     6121 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6181 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt ctttaagaga gttttccatg taacgaccca gcgaagcacc cagtgcataa
     6541 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7141 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggcccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcgataa
     8041 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     8221 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8821 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8881 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8941 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     9001 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     9061 cgagcagcaa tcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9121 ccgccagcag cacgcccgca ataaatgtac cgagcgccat cgacagcccc agcgcatcca
     9181 taaacaatgc ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9241 cagaagctgc aataaagcgg aataccggac gcagtaaata gcgcccacca atcagcatgc
     9301 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9361 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagta
     9421 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9481 acgccattgc ggtcgaagac atcgcaaggc caatgccacc gaccaccgcc gcctgccagg
     9541 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9601 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagctt ggagggattc aactcaaggc
     9661 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9721 cgctaataaa ccccagcccc cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca
     9781 cagcgccaat ccccagccgc gatgccaacg gcaccgcagc caccgccgcg aagagaaaca
     9841 gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9901 tgccagccag tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct
     9961 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
    10021 aaagggacgc agaacgtcgc tcatggggta gcgattcaat gcgtcataac ggtaagcact
    10081 ttccggctcg ccggtggtga tcacgctacg ccagtacttt cccgccagtt ggtttcctcc
    10141 cggcccgctg gcaaaaccac ggcttaatac ccggtccagc cactctttca gtagcgccgg
    10201 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10261 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcataaaggt cgtgcacggt
    10321 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10381 ttccggatgg gcaaacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10441 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10501 catacattac attatcataa tgataagtta acatagtctg aacatacggc accttatgat
    10561 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10621 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10681 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10741 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10801 atatgtcatt gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa
    10861 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10921 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10981 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    11041 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11101 cgatgccgtt atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat
    11161 ctctcacgac cgcgacttcc tcgatccgat cgtcgataaa attattcata tcgaacaaca
    11221 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11281 ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11341 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11401 gctcgagcgt atggagctaa ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11461 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11521 tggcgatcgc attattctcg actcgattaa actgaacctg gtgcccggct cgcgcattgg
    11581 tctgttaggc cgcaatggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11641 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aaactcggct acttcgccca
    11701 gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc
    11761 gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11821 taaagtaacc gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc tggtgctggc
    11881 attaattgtc tggcagcggc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11941 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg cgctggttgt
    12001 cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    12061 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    12121 acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aatgcgaaca gcgcccaggc
    12181 acgtaaagat cagaagcgtc gggaagctga gctgcgtgcg caaacccagc cactgcgtaa
    12241 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    12301 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12361 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg catggctgga
    12421 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12481 acgacgaccg atgccaatga attcagcagc agtgctgaat tcatccccat gcgcggcttt
    12541 agcaattgtc atctgcaaac catgctgccg cgtctgtttc gtcgtcaggt gaaattcacc
    12601 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatcttgc gtggagtgaa
    12661 aaccctgcac aggcgcaaca taaacctcgt ctggtggtgt ttcacgggct ggaaggcagt
    12721 ctcaacagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12781 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12841 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga atttggtcat
    12901 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12961 aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg
    13021 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    13081 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac gctgccgatt
    13141 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    13201 agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13261 ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat
    13321 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13381 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13441 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13501 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13561 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13621 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatattatgc
    13681 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13741 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13801 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13861 tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13921 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aggctaacga
    13981 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    14041 caaatatctg catacctacg acgaagccgt accgtggaat caggtaccgg ggacattcac
    14101 cccctggcaa cctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg
    14161 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    14221 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14281 acgagaagca gtgatggact cagtagtgcg ttcaatggaa gactatatca actacatcac
    14341 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14401 cccgttcgcg gcaaaaggta tcccgtcgct cgatgaaagc tttgtggtga tccattttcg
    14461 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14521 ccacatcaat acgttagtgg taccgggcgg caaaatgggt ctggcaatgg aattaattat
    14581 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14641 cggataaaat actatccggc ttcacaacgg gatagttaag tcacgcggca accacttcat
    14701 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcgg
    14761 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14821 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14881 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg
    14941 cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    15001 agttgccgtc cattaaaatc tgatgaccag aggcggattc gcccagaaaa gttaaccctt
    15061 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    15121 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    15181 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15241 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15301 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15361 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15421 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15481 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15541 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15601 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15661 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15721 acattctgat gcgtttgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15781 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaatctgg
    15841 caaaacaacc agacgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15901 aaattggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgctgg
    15961 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    16021 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    16081 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
    16141 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ctctcgttcc
    16201 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16261 cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac tggcaaaaga
    16321 tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagtt
    16381 ggggccattg cacgcaaaat tgcttcccgc atcgctgctg gcagccattt ttaccctcag
    16441 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg
    16501 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16561 actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16621 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16681 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16741 ctacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16801 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16861 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16921 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16981 cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt tcagccgcat
    17041 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcacgt gacttactgg ccgataaaca
    17101 gcggcgaatg ccattacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
    17161 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    17221 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac aaaatggcta
    17281 tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17341 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacgt tgatgctgat
    17401 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17461 tactattacc gcagtgtata ccctgcaact attgtggttg aacggcgagc aatacatcct
    17521 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct
    17581 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
    17641 tcaggaagcg attcgcttga ttcttagcga ggatccgcaa cctacgccac tggcctggca
    17701 gcgaatgcgg gtaaatcagg cacataacac tctgtataac tcattgaatc aggcgatgca
    17761 ggaaccggcg tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
    17821 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17881 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17941 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    18001 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    18061 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    18121 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    18181 aaggcattca cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaacca
    18241 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
    18301 ccagcgacgg tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct
    18361 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgta ctgtggtttc agctctgcgc
    18421 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18481 gcagatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18541 tgatgatatc aaacgccgcc cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18601 aaccgtgaga accaggatga aacgcagaag caatttctgc cgtggtcagc atggcgctaa
    18661 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acgccgtagt
    18721 gcatgtaagc aaacaaatcg ccggtccgcc ccatcccgca ctgtacttca tcaaacacca
    18781 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgctg
    18841 ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18901 tcaccgcttt cactgcgtgg agatcgttaa aaggaacgtg gatgatgtct gccggttttg
    18961 gcccaaagcc gtcggaatat tttggctgcc cgcccaccga gacggtaaac agcgagcgac
    19021 catgaaaagc gttatggaag gcaataattt tggttttgaa cggactatga cgcacacagg
    19081 cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    19141 ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc gccggttcat
    19201 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19261 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19321 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaatg aactctgccg
    19381 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19441 ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa tcacaaaata
    19501 tgcataaaaa atcattgaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
    19561 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
    19621 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcat
    19681 gctggtaatg agccaggttc caccaccagc gaatggtagc gtgtcacggt aagtggattt
    19741 gccagccccc gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcatg
    19801 acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg atgaccgagg
    19861 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19921 gcttcatctg gcgtacaggg gccgggtgat atgacaattt tttgtggttt aagggcgtcg
    19981 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    20041 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
    20101 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    20161 gctaaatatc atctgcagcg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
    20221 ccaggcctct ttttcgatac cctgccagct cagttgatac ccggcatgaa tcgccagttg
    20281 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20341 ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata
    20401 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20461 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccccg cccagtcgaa
    20521 aatatcctga tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20581 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20641 ctgctgctgg tggatgttca gccggttacg catgatatca aggccaggat aaagatacgg
    20701 atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctcttca aggcgcgcta
    20761 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20821 cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20881 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20941 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgaaaaatc attacggcag
    21001 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    21061 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    21121 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    21181 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
    21241 cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21301 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21361 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
    21421 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21481 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21541 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21601 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21661 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
    21721 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
    21781 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21841 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
    21901 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21961 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    22021 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    22081 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcggcggcg
    22141 atgttcattc tcggggtggt cagcggcatc accatgtcga ttggtacatt cctggtaaca
    22201 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt
    22261 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22321 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc
    22381 tgtgagttcc cggcgctggg caaacatgcg ccaaaaacgg atgctccggt agagaaagaa
    22441 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22501 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac
    22561 gcgggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
    22621 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
    22681 attctgatgt acgtctttaa caccggaaca ccagcacata tggcgtggtc aattctcgct
    22741 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22801 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg
    22861 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
    22921 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22981 cgtcaccgtc agcataacac cctgacctct cattaattgc tcatgccgga cggcactctc
    23041 gtcgtccggc cttttcctct cttcccccgc tacgtgcatc tatttctata aacccgttca
    23101 ttttgtccat tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
    23161 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    23221 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23281 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23341 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23401 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23461 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23521 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccgggcg caccgttttt
    23581 tatgacaagc tgatcatggc gaccggttcc tacccgtgga tcccgccaat caaaggttct
    23641 gatactcagg actgctttgt ctatcgcact attgaagacc tcaacgccat tgaatcctgc
    23701 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
    23761 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23821 gcggaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc
    23881 gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc aggaaggtgt cgaagcgcgt
    23941 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    24001 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    24061 ggtatcgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    24121 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac ctggctacaa aatggcgcag
    24181 gtcgccgttg accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
    24241 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
    24301 ggcgcacgta gctacgttta cctcgacgaa agtaaagaga tctacaaacg cctgattgtc
    24361 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggc
    24421 aacctgctgc aactggtgct gaatgctatc gagctgccgg aaaacccgga ttctctgatt
    24481 ctgccggcgc actcgggtag cggtaagccg tctatcggtg ttgataaact gccggacagc
    24541 gcgcaaatct gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa
    24601 ggctgccaca ccgttgcggc gctgaaagct gaaaccaaag cgggtactgg ctgcggtggc
    24661 tgtatcccgc tggttactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt
    24721 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc
    24781 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ctacggttgt
    24841 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24901 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
    24961 gacggcacct actcggtgat cccgcgttct ccgggcggtg aaatcacccc ggaagggctg
    25021 atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca ccaaaatcac cggctcccag
    25081 cgcctggcga tgtttggcgc acagaaagac gatctgccgg aaatctggcg tcagctgatt
    25141 gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    25201 gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg tcggcctcgg cgtggaactg
    25261 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25321 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
    25381 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggca
    25441 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
    25501 cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag aaaacctcga aggcggcatc
    25561 gattacctga aagcggtgat cattgacgac aaactggggc tgaacgctca tctggaagaa
    25621 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacaccg
    25681 tccgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag
    25741 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccggtaact
    25801 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25861 ctgaaaccgg cgtctgcgcg ctgttaggcg acgagcaggt cgcgattttc cgcccgtatc
    25921 acagcgacca ggtgtttgcg atcagcaaca tcgatccgtt cttcgagtcc agcgtgctgt
    25981 cacgcgggct gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    26041 agcgttttcg cttaagtgat ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    26101 acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    26161 gcaatgcctc ccctttttgc atggtcctgt aatgaccttc ggtataatgc aggacatttt
    26221 ttaaactttt tgttttattt tttgttttta ttttttaaag gataatcaaa tgtttacaga
    26281 cactattaat aagtgtgcgg ctaatgctgc acgcattgca cgcctgtcgg caaataaccc
    26341 gctcggcttt tgggtcagct ccgccatggc gggcgcatat gtgggtcttg ggatcatcct
    26401 gattttcacg ctcagtaatt tgctcgaccc gtccgtacgt ccgctggtga tgggcgcgac
    26461 ctttggtatc gccttaacgc tggtgattat cgccggttct gaactgttca ccggacacac
    26521 catgttcctc acctttgggg taaaagcggg cagcatcagc cacgggcaaa tgtgggcaat
    26581 cctgccgcaa acctggctgg gtaacctggt cggttccgtc ttcgttgcca tgctctatag
    26641 ctggggcggc ggtagcctgc tgccggtaga taccagcatc gttcactccg tcgcgctggc
    26701 taaaaccact gcaccggcaa tggtactgtt cttcaaaggt gcattgtgta actggctggt
    26761 ttgcctggca atctggatgg cgctgcgcac tgaaggggcg gcgaaattta tcgctatctg
    26821 gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac tctatcgcta acatgacgct
    26881 gttcgcgctc tcctggttcg gcaaccacag cgaagcctac acgctggcgg gtattggtca
    26941 taacctgctg tgggtgacgc tgggtaatac tttatcaggt gccgtattca tgggattggg
    27001 ttattggtat gctacgccga aagcgaatcg tccggttgcg gacaaattta atcaaactga
    27061 aacggctgcc ggttaattac taaggggttt ttacgtggat catttgccta tattttgcca
    27121 attacgcgat cgcgactgtc tgattgtcgg cggtggtgat gtcgcggaac gcaaagcaag
    27181 gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca ttagcgttta ttccacagtt
    27241 caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa gggccatttg atgaaagcct
    27301 tctcgacacc tgctggctgg cgattgcagc gacggatgat gacacgctta accagcgcgt
    27361 cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgta gtcgatgcgc cgaaagccgc
    27421 cagctttatt atgccgtcga ttattgaccg ctcaccgcta atggtagcgg tctcctctgg
    27481 cggcacctct ccggttctgg cgcgcctgtt gcgcgaaaaa ctcgaatcac tgctgccgtt
    27541 gcatctgggc caggtggcga aatatgccgg gcaattacgc gggcgagtga aacaacagtt
    27601 cgccacgatg agtgagcgtc gccgtttctg ggagaaattg ttcgttaacg accgcctggc
    27661 gcagtcgctg gcaaacaacg atcagaaagc cattactgaa acgaccgaac agttaatcaa
    27721 cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt gcaggtccgg gcgatgccgg
    27781 gctgctgacg ctgaaaggac tgcaacaaat tcagcaggca gatgtggtgg tctacgaccg
    27841 tctggtttct gacgatatta tgaatctggt acgccgcgat gctgatcgcg ttttcgtcgg
    27901 caaacgcgcg ggataccact gtgtaccgca ggaagagatt aaccagatcc tgctgcggga
    27961 agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc gatccgttta tttttggccg
    28021 tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt ccgttctcgg tggttccggg
    28081 tattaccgca gcttctggtt gctctgccta ttcgggtatt ccgctcacgc atcgcgatta
    28141 tgcccagagc gtgcgcttaa ttaccggaca cttaaaaacc ggtggcgagc tggactggga
    28201 aaacctggcg gcagaaaaac agacgctggt gttctatatg gggctgaatc aggctgcgac
    28261 tattcagcaa aagctgattg aacacggtat gcctggcgaa atgccggtgg caattgtcga
    28321 aaacggaaca gcagtcacgc agcgcgtgat tgacggtacg ctcacgcaac tcggtgaact
    28381 tgctcagcaa atgaacagtc catcgctaat tattattggt cgggttgttg gcctgcgcga
    28441 taaactgaac tggttctcca accattaatt taacccgggc cagagaattc tggccttctt
    28501 aatgactctt tttatatatt cagcaaataa aacatagccc cttaataaat attatctgct
    28561 aatgggttat attcaccgtt acagcgactt acaaaattaa gtcattcgct cactgacgcc
    28621 aattaaatat tcctgtatcg ttcttaacga gcagtctgct tatattcata taatcaatga
    28681 atattaatta atatataata catagggaat gtaaatgaac aaatttatta aagttgcact
    28741 ggtaggtgca gtactggcta cgttaactgc atgtactggt catattgaaa accgtgataa
    28801 gaactgctct tacgactacc tgctgcaccc ggcaatttct atttctaaaa tcattggcgg
    28861 ttgcggtcct actgcacagt aagtctctga cagaaatcgg ctaacaccgg tttaattgcc
    28921 cgacgccctc tggattttcc gagggcgtat ttgtttctat gtccttcttg cccccgcccg
    28981 taaaaatatt ttcactttaa attcaatttg ataactacat ccattagcca cagcgcgatc
    29041 ctgtgcgaaa ttttgtgatc ttcctccaca ttacataaca tcatatgttg ttatattcat
    29101 catgcattgt catgttacct tttaaatgac tgcaaactct cccctacaac gtattggaca
    29161 agaaaaaggt atcgctatgg gaagccagga actccaacgc aagctcggat tttgggccgt
    29221 tcttgcaatc gccgtcggga caaccgtcgg ctccggtatt tttgtatctg tgggtgaagt
    29281 ggcaaaagca gcgggcacgc cgtggcttac ggtgctcgcg tttgtcattg gcgggttaat
    29341 tgtgatcccg caaatgtgcg tctatgcgga actatccacc gcttatccgg aaaatggcgc
    29401 agattatgtt tatctgaaaa atgccggaag ccgaccgctg gctttcctct ccggctgggc
    29461 cagcttctgg gccaacgatg cgccgtcatt gtcgattatg gcgctggcga ttgtcagcaa
    29521 tcttggcttt ttaacgccta tcgatccgtt gctcggtaaa tttatcgccg ccggattaat
    29581 tatcgccttt atgttgctac acctgcgctc cgttgaaggc ggcgcagcgt ttcagacgct
    29641 aattaccatc gccaaaatta tcccgttcac tatcgtcatt ggccttggga tcttctggtt
    29701 taaagcggag aattttgccg cccctaccac cactgcgatt ggcgcaacgg gcagctttat
    29761 ggcgctgctg gcggggatct ctgccaccag ttggtcgtat accggcatgg cctctatctg
    29821 ttatatgacc ggcgaaatta aaaaccccgg aaaaaccatg ccacgagcgc tgattggttc
    29881 ctgtctgctg gttctggtgc tctacaccct gctggcgctg gtgatttccg gcctgatgcc
    29941 cttcgacaaa ctcgccaatt ctgaaacgcc gatttccgac gccctgacct ggatccccgc
    30001 actcggcagc accgctggga tctttgttgc catcacggcg atgatcgtca ttcttggttc
    30061 gctttccagc tgcgtgatgt accagccgcg gctggaatac gcgatggcga aagacaacct
    30121 gttctttaaa tgcttcggcc atgtgcatcc gaaatacaac acgccggatg tctccatcat
    30181 cctgcaaggg gcgctgggga tcttcttcat cttcgtttcc gatctcacca gcctgctggg
    30241 ttatttcacc ctggtgatgt gtttcaaaaa taccctcacc ttcggctcca tcatctggtg
    30301 tcgtaaacgc gacgattaca aaccgctgtg gcgtactccg gctttcgggc tgatgaccac
    30361 cctcgccatt gcgtcaagcc tcattctggt cgcctcaacc tttgtctggg caccgattcc
    30421 cggccttatc tgcgccgtca tcgttattgc tactggtctg cctgcttacg ccttctgggc
    30481 gaagcgtagc cgccagctca acgctttgtc gtaatgttat ctggagaaaa taaaatgttg
    30541 gatattgata aaagcaccgt ggactttctg gtcaccgaaa atatggttca ggaagtggaa
    30601 aaagttctca gccatgacgt tccgctggtg cacaccatcg tggaagagat ggtgaagcgc
    30661 gacattgatc gtatttattt cgttgcctgc ggatcgccac tcaacgcggc gcaaacggcg
    30721 aaacatctgg cggatcgctt ttccgatctt caggtctacg ccatttccgg ctgggagttc
    30781 tgcgataaca ccccgtatcg cctcgacgat cgttgcgcgg tgattggcgt ttctgactac
    30841 ggtaaaacgg aagaggtaat caaagcgctg gagctgggcc gggcctgcgg cgcactcact
    30901 gcggcgttca ccaaacgcgc ggacagcccg attacctcgg cggcggaatt tagcattgat
    30961 tatcaggccg actgtatctg ggaaattcac ctgctgctct gctacagcgt ggtgctggag
    31021 atgatcaccc gcctcgcgcc gaacgcggaa atcggcaaga tcaaaaacga tctcaagcag
    31081 ttgccgaatg cgctcggtca actggtacgc acctgggaag aaaaaggccg ccagcttggt
    31141 gaactggcca gccagtggcc gatgatttat accgttgctg cgggtccgct gcgtccgctg
    31201 ggttacaaag aaggcattgt aacgctgatg gaatttacct ggacgcacgg ctgcgtgatt
    31261 gagagcggag agttccgcca tggcccgctg gagattgtcg aaccgggcgt tccgttcctg
    31321 ttcctgctcg gcaatgatga aagtcgccac accaccgaac gcgccattaa ctttgttaaa
    31381 cagcgtactg acaacgtgat cgtcatcgat tacgccgaaa tttcgcaagg gctgcacccg
    31441 tggctggcac cgttcctgat gttcgtgcca atggagtggc tctgctacta cctgtctatt
    31501 tacaaagatc acaacccgga tgaacgccgc tattacggtg gtctggtgga atattaatcc
    31561 ctctcccggc ccggcaacgg gccgaattta cgcaaggagt tacccgatga aaacaggtat
    31621 gtttacctgc ggccaccagc ggctgcctat tgaacacgca tttcgtgatg caagcgagct
    31681 gggttacgac ggcatcgaaa tttggggcgg tcgcccgcac gcgttcgcgc cggacttaaa
    31741 agcgggcggc atcaaacaaa tcaaggcgct ggcgcagacg tatcagatgc cgattatcgg
    31801 ctatacgcca gaaaccaacg gctatccgta taacatgatg ctgggcgatg aacatatgcg
    31861 tcgcgaaagc ctcgacatga tcaagctggc gatggatatg gcaaaagaga tgaacgcggg
    31921 ttatacgctg atttccgcgg cccacgcggg ctatctcacg ccacctaatg ttatctgggg
    31981 acggctggca gagaacctaa gcgaactgtg tgagtacgcg gaaaacatcg gcatggatct
    32041 aatcctcgaa ccgttaacgc cgtatgaatc gaacgtcgtg tgtaatgcta atgatgtgct
    32101 tcatgcgctg gcgctggtgc cttcgccgcg cctgttcagc atggtcgaca tttgcgcgcc
    32161 gtatgtccag gcggaaccgg tgatgagtta tttcgacaaa ctgggcgata aattacgtca
    32221 tctgcatatt gtcgacagcg acggggccag cgacacgcat tacattcctg gagaaggcaa
    32281 aatgccgctg agggaactga tgcgcgatat tatcgaccgg ggctatgagg gttactgtac
    32341 ggtggagctg gtgacgatgt atatgaacga gcccagactc tatgcccgcc aggcgctgga
    32401 acgctttcgc gcgctgctgc cggaggatga gagatgaaaa ccctggcgac aatcggcgat
    32461 aactgcgtcg atatctaccc gcaactgaat aaagcgtttt ctggcggtaa tgcggtcaat
    32521 gtggcggtgt actgcactcg ctacggcata cagccgggat gcattacctg ggtgggtgac
    32581 gatgactacg gcacaaagct gaagcaggat ctcgcccgca tgggcgtcga tatcagccat
    32641 gtccatacga aacacggcgt taccgcacaa actcaggtgg aactgcacga caatgatcgc
    32701 gtttttggcg actacaccga aggcgtgatg gccgactttg ccctgagtga agaggattac
    32761 gcctggctgg cgcagtatga cattgtgcac gcggcaatct ggggacatgc ggaagacgca
    32821 ttcccacagc tgcacgctgc gggcaaactt accgctttcg acttctccga caagtgggac
    32881 agctcgctct ggcagacact ggtgccgcat ctcgattttg cctttgcctc cgcaccgcaa
    32941 gaagacgaag cgctgcgtct gaagatgaaa gcgattgttg cccgtggcgc aggcgtggtg
    33001 attgtcacgc tgggtgaaaa cggcagcatt gcctgggatg gcgcgcagtt ctggcgtcag
    33061 gctcctgaac cggtgacggt tatcgacacc atgggtgccg gagattcgtt cattgccgga
    33121 ttcctttgcg gctggtctgc ggggatgaca ttaccgcagg cgatggcgca gggaacggcg
    33181 tgcgcggcga aaaccattca gtaccacggt gcctggtagg tataacgttg gcgtgagcat
    33241 cttcacgcca acgtgctgtt acttgccgga aaacgaccct ataatccgag taattcattc
    33301 tttatttcag ggtcgattat gtcagctacg gaccgctact ctcatcaact cctctacgct
    33361 accgtccgcc agcggttgct ggatgatatc gcgcaggggg tttaccaggc cgggcaacag
    33421 atccctaccg aaaacgagct ttgtacgcaa tataacgtca gccgcattac tattcgcaaa
    33481 gctatcagcg acttagtggc agacggcgta ctgatccgct ggcagggcaa aggcaccttt
    33541 gtacaaagcc agaaggtcga aaacgccctg ctgaccgtca gtggttttac cgattttggc
    33601 gtctcacaag gcaaggcgac gaaagagaaa gtgatcgagc aggaacgagt cagcgccgcg
    33661 ccgttttgcg aaaagctgaa catccctggc aacagcgaag tgttccatct ttgccgggtg
    33721 atgtatctcg ataaagagcc gctgtttatt gatagttcct ggatcccact gtcgcgttat
    33781 cctgactttg atgagattta cgttgaagga agctccactt atcagttatt tcaggagcgt
    33841 ttcgacacgc gagtggtcag cgacaaaaag accatcgata tctttgccgc cacccgcccg
    33901 caggcaaaat ggctgaaatg cgaactgggc gaaccgttgt ttcgcatcag caaaatcgcc
    33961 tttgatcaga atgacaaacc ggtgcacgtc tccgaactct tctgccgcgc caatcgcatc
    34021 accttaacga ttgataataa aagacattaa ccgtaggccg gataagatgc gccagcatcg
    34081 catccggcga tgctggcgcg ttgaatttta catcccgtac gttcccctca ccctaaccct
    34141 ctccccaaag gggcgagggg accgttcagt acggaattta gcttgacggt gctatccagc
    34201 attaaatact ggcaatactc tcacgcaaaa tccgcagcac cgtctcttca ccgctgcgat
    34261 tcgggttaat gcgaatcgca caatgttctg attgtggatt cgcctggcga aacgttccgg
    34321 aaaggcgata aaagagcggc gggatttcat atttcgactc tgcacccacc gggtaaggca
    34381 aggcaccgcg cttttgcgcc tcttccagca ctctggcggc aatcggctga tgaaactcga
    34441 caatcaacac cttcgactgc gcattagcaa tcaccgcgct tttcacttcc ggcaccgcac
    34501 caccgttaag caaagccagc aaccgttcag atacccctgc ctgcaccgcg tgcatcactg
    34561 gcgcaaacac cagaccacgc aatacttcca gcgcctgtgc gccctggatc tggctaccgc
    34621 cggagtaaag cgtggcgcga atacggttga taacatcagc atcgccgacc actgcaccaa
    34681 caccctctgg cccaaatagc ttgaagcagg aaaatgtcga gacattcgcg ccgcattcac
    34741 agccgattcg cgccaccttc atcaccgcat agttgtcatc ggttaacgct ggaacacctg
    34801 ccgcgcgcaa cgttgccagc acatctgcca gcacgtagct gtcctgcggc tgctggcgcg
    34861 tatgctgcac cagcgccgca tccggttgtt gctcgtcgac gacctgcttc agtgccgaca
    34921 ggtcattgaa atcaacagta ataagcgtca gccccatctg ctcaataata acccgtgtcg
    34981 tcgggtaaac aggcgcgtca tgcaccagaa gacgctgccc cggtttgagc aaagccgcca
    35041 gcccggcgcg aatcgcgcca gtccccgcgc cctgcaccag cgccgcagcc tgtgcgtgaa
    35101 atgcatcagc cagcacctgc tccacccgtt gggtaatacg cggttgattc agacctggag
    35161 ttaaccctaa atcaccgcca gtaagaaact cgctgccggg gaaatggcga caaatgctat
    35221 ccaccagcgc aaactgcttt tgctgcgcct caataatcgt caggctttgc agaggaaacg
    35281 tcttcatagg tgccttcctt aagccggaac gaacaggcca agccagtaaa gaacattcag
    35341 cagaataccg gtgatcatta ctgccacgac cggtgccgcc attttctgta ccggacggcc
    35401 cagggattcg ttgaggaagt aaatggcaac cgcgatagag aatccggtat aacccgccat
    35461 cttaattgct gcgaaaatcg aaccgaccag cagcgccact tccatcagca tattcatggc
    35521 gttacggatg ttatccgacg cattacgcac cgacgggtag cgtcccagcc atttgccgat
    35581 cgaacgaagc agcaagactt ccgccgaaat aaccactgcg cctaataccg ccgcaaccat
    35641 cggatttggc gagagatagc ccaccgcata aacaaaggta aagcccgcaa ctgcatacac
    35701 gccagtggct aacgcggtgg tggcaatcaa cggcacaaaa cccagtccgc gcataaattc
    35761 tgccagagcc gcctgattaa tcagcgtttg cgattgttcc ggcgtcacgc ctgcggaata
    35821 cgctttctcc agtgtgaaga tcgaaacttc gctaccagca aaaatcttca tactggcaac
    35881 ggcggcaatc aatgctccca cgatggcgat ataaggtaag tttttgataa tccgcgacgt
    35941 gcgctcttca aacaccgaaa gcccgctggc atcgatgtca ttttcatcac gatgacgcag
    36001 gtcgtgagtt atcgcgatcc ccagcagcat caccatgcca ataaagattt cgatggattc
    36061 agggttaaga tgtgggaaat agcgcacgac taccacacgg gtcatcagaa caaccacggc
    36121 ggcgaccaga ctttgcttcc agccaaactg gtagaaaatc gccaccagcg ggaacagtgc
    36181 aaaagcagaa accaccggcg agcttaattc cccgaggcta cctaatacat ccaccggcag
    36241 cgcggtcagc agctggttta ctggcaacag gcaagtaagg atcaacacgc cccagatagc
    36301 gccaagacca aacgccatca ggctgtttat cgccagtacg ccgaggatat cggtaggaag
    36361 aaagagtaac catgcgttga gcagcccggt tttcagggta aaagagatcc ccaccgaggc
    36421 cacaaaacca atgctcagac caaaagcgat actccccgcc tcgcgacgat tcatatagcc
    36481 ttcaatcagt tgcggcagga tcgggcggat gccgtcatga aaaacagccg ccgagcgatg
    36541 cgccagaagc gatgtcatac ccgtcaggca cgccaccacg ataatctgaa tatacagatc
    36601 catagtcgtg ccttttattt cagatggtta atcagcatcg gaatcgcgtg ttcgacgtgc
    36661 tcaacggaaa ggccaaacgc cactttccct tcagcgatca ttttggcgat atgctcgtct
    36721 ttcgctttaa tgcctggttt ggcaatggtg caacttttgt tatagccaat caccgcgatg
    36781 gcaatcgaca atgccgcgcc cgcaccggta ttacacgcac cgatgtagta atccagttgc
    36841 ccggatttca ctttcattgc cgcttccatg tcgttgtgaa tgaaaacttc aaaactgcca
    36901 ggagccgtcg cttcaatggt ttttttaatc tgctcacgct gtaagcctgc aacgccaatc
    36961 tttttcatga gtctgtcctt attggaaaaa ttgagaggga ttttcacgta acatcacatc
    37021 cacatcggcc tgactgaatc ctgactggcg caattgcgga ataaaggtgg tcaataaata
    37081 gtcatagcca taaccaccat tggcttttaa atgggagcgg cgcgtaatat ccatcgacag
    37141 catgacgcgg ttcagcaacc cacggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc
    37201 gtccgggtag taactgttct taccaatggt gtcgaactgc acgtacgcgc cgagatcgat
    37261 catcttcaaa atgttgtcga gattgtcttt cagatcgcag tgaccaacgg tgacgcgcga
    37321 aagatcaact ccgtgggctt gcaacagagc cagttgttcc agccccatgg tgctgaacga
    37381 agtatgcgtg gagatcgggc gtccggtctg gttatgcgcc agcgcagcgg caataaacac
    37441 cttctcttcc agcggcgtaa tcttcccttc gctagagccg atctccgcga taatcccggc
    37501 tttcaggtcg gtgccatcga taccctgttc aatttcatcg accatctcct gcgccagttc
    37561 ctgcacgctg cgggtcgcca catgctccgg gaaaaaagcg tcctggtaat aaccggtgca
    37621 ggccaccacg ttgatccccg tctcgcgcat tacatcgagc ataaattgcg cattgcgccc
    37681 catgtaacgg ttggtcatct caatcacatt acgcacgccc cgggtcatca ggtcgttcat
    37741 ctcctggcaa atgaacgcat actgatcaag gcggcagtcc acgttgtttt taaagccgga
    37801 gagatcaata tgcagatgct catgggccag ggtgtaaccc gtcggatcaa tactcatagg
    37861 gtgtctcctg caaaccggag ggaggaaaga aaagagcgac cattttgtgg cggtggcgca
    37921 cggaaaaatt cacacacggt agcccccaca tcagaaagcg tggtgcgtac accgagttgc
    37981 gtgtggacca gcccttgctg ataaaccagc actggcacca cttcacgggt atggtggctg
    38041 tgaccaatgg tcggatcgtt accgtgatcc gccatcacga ccaggcaatc atctggctgc
    38101 atcgcctcaa caagccgggc aaggttacgg tcaacgacct gcaaacgttc ggcataacgt
    38161 gcgacgtctt ctgcatgacc agcgaggtcg gtttcctgga tattgatgca aataaacgcc
    38221 gtcggatgcg tgttaaattc gtcgagggtg atatccataa tccgctggct atccaccagg
    38281 ttttgccagc tcacgccata aggattgctg acgatatctg ccaccttacc taccagcacg
    38341 gttggcacgc ccgcctcata cagtttttgc ggcacctgca ctttttcatc aacaccgtag
    38401 cccatatgca cgacctggaa gccgttgtca taagcgccag aacgcggcgc attaatacca
    38461 ataaagcgcc cttctttgct ttctgcggca tcgagaatac gctgactgtc ggttaacaag
    38521 ccgccaaatg taatgacccg accgacctgt acctgctcac gcacgatacg accaattttg
    38581 atcgcgtcgt caaaagagat cacagagaga ttggcggtaa tgttatagac ctggcctaaa
    38641 tccgcctcga gattatcgcc aatcgcaacc gcctgattga cccacagaaa ttgcagatca
    38701 tcgccacggc gctccacctg ccagccagcg gaaactaatg cctgctcaac acggccaatc
    38761 acatcgcgaa agggcatccg cagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc
    38821 cccataaagg tatcgccacc ttcatgttgc agctctgcca ccccccaggt tgcggaatct
    38881 gacggttgca tatcacctgg tgcataaccc aatgcgttga ttagccccag cttctccagc
    38941 gctggtagct gcaaatgcgg caactggctc aggatgtgac cacaggtatt cgccccggca
    39001 tcttgcgggc gcaccagcgt gacatctttc attgccccta cgccaaagct atcaatcact
    39061 aacaccacaa atcgcgccat tacgccccca gagagtttcc aagactgtca taacgaccga
    39121 cgattttcgg ttcgccacgg tgaataccgg acaccagcac cacatcgcta cgagtgacaa
    39181 aaatctgcgt gcgaaaacag agcactactg cactgcttac cggaaactcg cctgccagcg
    39241 gcagggtgta gtcgatactg ctgtcatcca cagttttgag attggtttca gtaatctttt
    39301 gattttctgg cgtaaacacc agcgcatgtt gcgcatgacc acgacggtag taaccgccgc
    39361 cgtagcagta gctgtcgcca cggaaatgat gggagatttc acttaaccaa agcatcgcta
    39421 tatattcagg ctgatcgccc tgctggtttg ccggaatagt gcccgtcagc gcatgaccgg
    39481 gttcggcatg agtcacaccg tattccgcca gcatcggcag cgaagtgcag ctggtcgcag
    39541 aaggcgcgtt cagttgctca atcgcaatac cagatttcgc cagttgatcc cgtgcttgta
    39601 ccagcgtgtg aagattcggt gtcggcaaaa ctttcccggc agcctcatcc caaagcaggc
    39661 aagggaaatg ggtaagtccg gctaaatgca gacccggcag tttcttaatt tcagcaacca
    39721 cctcgtgcag cgaatgctgt acaaaaccgc tctcctgccc cggataaaga aaatcatcgt
    39781 cgctataaac tttgagcagc acaaactgca cacgtccggc cttcaccgct gccgcagaaa
    39841 cttcccgcgc tttgtcgaga gtaaacacgg tgatgacgtc ggtgccctgt tcaacggcgt
    39901 cagcaacctg atgacaaggg atttgtacca gatgcccctg atgcgccacc gacaaaccag
    39961 cgcggcgcat gactcgcgcc tctttgtaat ccaccgccac aatgccgctg taacctaatg
    40021 ccagcaattt ttccgccagc catgggttac ggccaaactg cttggtcatc agatacagtt
    40081 caatgccgta aagccgcgcc gtctcaatca gtcgcttacc gttttccagt acctgatcca
    40141 cgtcgatcac ccagctgtcc ggggcgatct tgccctgctg ccacaaactt agtgcggcgg
    40201 aaatcagcgc cgggttctgg cgtttcaatg cttctacaaa catcttgttg cctcgtttct
    40261 gttccttgaa ttaaatattc atttttttga atatttaggt tgtgcgacgt ggatttgtcg
    40321 ggagcttcaa acctcttcgt tagcagccat ccataatcca taaaggttgg ccagcaaata
    40381 gccttcttca cacgggttaa cttccagtgc gaattccttc aacaacacct gatgcaattg
    40441 cacaacggct tgccagtggc tggattgcgc cagctctgcc agcaattcgt tatccagcgg
    40501 ctctatttct tcaccacggc gactgcgcat cagtgcactc gccatatgtg tcatcgccat
    40561 cgttccttgc tcactgcgca ccggcagatg gcactctgtt tccagtacgt tgacgacctg
    40621 catcatgcct ttgcagatgt ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct
    40681 ggtttccata tcagatgcct ttcgtttaat aactcggttc ctgttcccca ctcactaccc
    40741 gttgttgatg ggcgagcagg gcgtgtttat ctacaacggc acgcagcagt tgttgcattg
    40801 ggtaatcatc gactcgcgtc agaaccacgg cttcggtggc ctgtaaaaat cgcggatcgt
    40861 ctgtgagcgg cgtcgcctct aatcccagca tggtcagttc gttttccgcc accacgttcc
    40921 agatcaccgc atcgacatcg cctttaacaa tgcgttgtaa actctcgtga taagagagat
    40981 cgacacgttc cacatcacta tcgccaaaaa agacatcggt catgattttc tgatccgccg
    41041 aacggttatc cagccccacg cgcttcacgt ttgcggactc gcctttacgg caaatcaact
    41101 ggtgctcgcc aacgtaggtg tgcggcccca actccagcgc gaggcaaaac cctttttgcg
    41161 tgagataact ttccgccgcc agtcgggaaa ccaccgccat gtcatacacg ccgttaagta
    41221 aacactccac gcgaatatcc gcgccgcgca tatgcgcata gtaaaaagga atgccatcaa
    41281 actgggcttt caatccgctc gctaaacctt cgtacaagcg ggtatagggc aaaggcatcg
    41341 cacataccac gttgttgata tccacatgac tcagcaatgc tttgttatcc atctcgacca
    41401 gataactgcc attgcgccca cggcgttcaa tccgtatcgc tccgcttgat tccagcgttt
    41461 tcaacgcggc ctgcgtcagg ccaacggatg aacggcattc agttgccagc tcatctatgg
    41521 ttttcaacct attaccgcat ttttcaccca acaaataacg ggccagcgtc gtgataacga
    41581 cgccttcttt tttgataaac gttcgacgca taatatattt tcaataaaat gaatgtttat
    41641 atcttcatta aaatgaagat aagtggcaac tgtgagatgt ggaaacggcg aggcgcttca
    41701 cattttttcg gattattgca aatgaggata aaaaaaccgg gtttccccgg tttcagagtg
    41761 atgacgaagt gatttattcg acaatgcgag caaagggtaa ttcccactgg cgcttgcctg
    41821 ctcccctcac cctaaccctc tccccaaagg ggcgagggga ccgtccgagc gttttgatat
    41881 tacggcttcg ccacaaaacc aatcgcttcg tacaccgctt tcagcgtacg ggaagcgtgt
    41941 acactggctt tttctgcgcc gtctttcatc acctgttgca ggaaggcttc atcgttgcgg
    42001 aaacggtgat agcgttcctg caattcagtc agcataccgg aaacggcatc agccacttca
    42061 cctttcagat gaccatacat cttgccttcg aactgttttt ccagttctgg aatgctctgg
    42121 cccgttaccg cagaaaggat atccagcagg ttggaaacgc ccgctttgtt ctgcacatcg
    42181 tagcgaacta ccggcggctc gtcggagtca gtgaccgcgc gtttgatttt cttcactacc
    42241 gatttcggat cttccagcag gccgataacg ttattgcggt tatcgtcaga cttggacatc
    42301 ttcttggtcg gctccagcag cgacattacg cgcgcgccag atttcggaat aaacggctcc
    42361 ggcaccttaa agatctcgcc atacagcgcg ttgaaacgct gggcgatatc gcgactcagt
    42421 tccaggtgct gtttctggtc ttcacccacc ggtaccagat tagtttgata cagcaggatg
    42481 tccgcagcca tcagcaccgg atagtcaaac agaccagcgt tgatgttctc ggcataacgc
    42541 gcagatttat ctttaaactg cgtcatgcgg ctcagttcgc cgaagtaggt atagcagttc
    42601 agtgcccagc ctaactgcgc atgttctggt acgtgggact gaacaaaaat ggtgcttttc
    42661 tcaggatcga taccacaagc cagatacaag gccagcgtat ccaacgtcgc tttacgcagc
    42721 ttctgtgcat cctggcgcac ggtgatcgcg tgttggtcaa cgatacagta aatgcaatgg
    42781 tagtcatcct gcatgtttac ccactgacgc agcgcaccca tgtagttacc aatggtcaat
    42841 tcacctgagg gctgtgcgcc actaaaaacg atgggcttag tcatttttcg attcctgatt
    42901 ttcgctatgc ggaagcccta atgcgggcag aaggtcattt atagactgat aaattacatc
    42961 aggctggctg agatcgatag cctcgccgta gttatatccg taggttaagc caactgatgg
    43021 gcaacctgcc gcttttgccg cctgaatatc attgcgtgag tcgccgacaa acagcatctg
    43081 ttgtggggca attcccatcc gctcagccac cagtaacagc gggtccggat gcggtttttt
    43141 gttttgcaca tcatcgccgc cgataaccac gctaaagtat ttggcaatat ctaaggcttc
    43201 gagcagcggc gcgacgaacg gcgtcggttt gttggtgacc aggcctagcg gcaggccttt
    43261 agcctgcaac gcgcccaacg tatcggcaac gtgcgggaac aaaaacgtcc cctcttcggc
    43321 aacctcgcca tagtagcgat cgaacagttt acgcagaata cgtacctgtt cttctgccgg
    43381 aatgtcgtca tcaacgggcg gtttacccat tgttttacgc tgagtcgcac gttcctgacg
    43441 cgcccaggtc aatgcgcgct ccatcagaac atctgcgccg ttaccaatcc aggtaataac
    43501 gcgttcttca cctgcgacgg gcaactccag cgcatacagc gccatatcta ccgcagcagc
    43561 aagaccggga gcactgtcga ccagcgtacc atcaagatca aaagcgacgc cgcgaatatc
    43621 ttcaaactta ttcatgactt aactttgcca gttcactgcg catttcatca atgacttttt
    43681 tgtagtctgg ctggtcgaag attgccgaac cggcgacgaa catatccgcg cccgccgcag
    43741 cgatttcgcc aatgttgttc accttcacgc caccgtctac ttccagacga atgtcaaagc
    43801 cagactcgtc gatacgacga cgtacttcgc gcagtttatc cagtgtttga ggaatgaacg
    43861 actgaccgcc gaaaccagga ttgacggaca tcagcaggat cacatccagc ttatccatca
    43921 cgtaatccag atagctcagc ggtgtcgccg ggttaaatac cagtcccgct ttacagccat
    43981 tttctttaat cagttgcagc gtgcggtcaa catgctcgga ggcttctgga tgaaaggtaa
    44041 tgatgctggc accagcggca gcgaaatcag gcacaatgcg atcgacaggt ttcaccatca
    44101 ggtgtacgtc gataggggcg gtaatgccgt agttacgcaa ggatttcagc accattggcc
    44161 caatcgtcag attgggaaca tagtggttat ccatgacgtc aaaatgcacg acatcagcgc
    44221 cagctgccag ggcttttgcg gtatcttcac ccaggcgggc aaaatcagcc gacagaattg
    44281 agggggcaat caaatactgt ttcatccgct tctccttgag aattattttt tcgcgggtga
    44341 aacgactcct ggtttgtaca aagccagcag ttcgtccacc tttttacgtg tgccgccgtt
    44401 gctgcttata ctgcgtcgaa ctttgacgac atgcaatttt gcgcgctgat accactcacg
    44461 cgttaacatc gtatcgtgat tggagatcag cactggaata tggcgctcaa ccagaccttc
    44521 ggcgatctcc gccagatgcg cttgttgttc aagcgtaaaa ctgtttgtgt gatacgccgt
    44581 aaagttggcg gtcgcagaca gcggtgcata aggcggatcg caatagacga cggatgcatc
    44641 atctgcgcgc gccatgctat cggcgtaaga ctcacaatag aaaaaggcat tctgcgcttt
    44701 ttcagcgaag tgatacaact ctgcttccgg gaaatagggt tttttgtagc ggccaaacgg
    44761 cacgttaaac tcaccgcgca gattgtaacg acacaggccg ttgtaaccgt agcggttcaa
    44821 atataaaaac agtaccgccc gacggaacgg atcctggctt ttgttgaact cttcgcggaa
    44881 ctgatagtag acctcggcgc aatttgtttc gggaacaaac agctcgcgtg cggcctgtac
    44941 gtactcatca gtacgcatct tcacaatgtt atagagactg atcaggtcgc tattgatatc
    45001 ggcaaggata taacgagaaa agtcggtgtt gagaaacacc gacccggcac ctacaaaagg
    45061 ctcaaccaga cattcgccct tgggcaaatg ccgtttaata tcatcaagca ggggatactt
    45121 gccccctgcc cacttcaaaa aagcgcgatt tttcttcatg ctgactaact aattacacct
    45181 tctccggctg tggagaaagc tccgacagca tccagcgctt caggcaactc ccgcaacgat
    45241 aacccattgc gggagataac cttgattact tcagatcggc ctgtacctga cgcagcggtt
    45301 tcgcccacgg gtttttggcc tgaacatctg ctggcaatgt agataccgct tttttcgcct
    45361 cttctttcga agcgtacacg ccagaaacca ggacatacca cggctgacca ttacgcgtcg
    45421 tttcatagac aacgtagttt ttcagattct ctttcttcgc ccaaccgttc aggttgtcgt
    45481 agttagagga actgctcagc tgcagagtgt aatggctgga cggtgccgat ttcaacgaac
    45541 caacattacc tgcggtcttc cctccagcgg ctggtgttgc cgtggtttgc gccggggatg
    45601 ccgtctgtac tggagccgta gtcgccgtct cttttggtgc tggcgcagaa gtcgcagccg
    45661 gtgccttcgt gctcgccaca ggagcagctg gttcagtacg cttcggcgtc tgtgctaccg
    45721 gcttcggttc cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac
    45781 gagctggacg cgtagtggcc ggacgttcag cagtttgcgt tttcgccgtg tcacgcgatg
    45841 cattgccatt gcgtactggc gcgacagttg ctggttcagt cggcaatgta gaattgaccg
    45901 ccacattgtt caactgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct
    45961 gcacttcaac acgttgttga ccatccgttg ccgccggggt ttgcccttga gtcggcgtag
    46021 aagagatcgg tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg
    46081 ctggctgcac cccattcgcc tgatcggtcg cattgcctgc aagatcaata ctcttctcgc
    46141 cagacgcggt ttgatcgctg gaagaggtcg agggggcttt tagcgcagaa ccgataccga
    46201 tgatcaacag cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg
    46261 gtttactggc tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg
    46321 tttcatcttc ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt
    46381 gcgcacgagt cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct
    46441 gcggttcgcc acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac
    46501 gacgatcgct gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc
    46561 ccactaaaag gttaatgctt accacattgc aattaacctg aagctaatag accgcttgat
    46621 aagcggcctg acctttcttg ttgttacgct gattgactat cggcaatggc gttaagaaca
    46681 agctcgtgcg aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag
    46741 cgtatctctc ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg
    46801 gacatttcgc gcggcccatt gaccggtaac ccagcccgct tgagcagggt tataatacgc
    46861 tgcgtttcgg cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata
    46921 cccgcagcga ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca
    46981 tgaccaaagg tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg
    47041 gcgacaactt ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg
    47101 tccagacgca acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga
    47161 ataatgccgt atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt
    47221 ttcagacagt cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt
    47281 ttaccgaggg gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt
    47341 aacgtcgtcg ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg
    47401 gtcagatcgc ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc
    47461 ttttgtaaca acgccgtaaa gacggtatcg agtacagcca ggcttttata ctgctcgccg
    47521 tcagggagga taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta
    47581 tcgagataca gaggagccag ggtttcgttg gtgaccaaca tgacctgctc gcccgatttc
    47641 agcggtaaga atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta
    47701 cgttccccga gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac
    47761 ccgcagacga gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt
    47821 ttgcaaccac tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat
    47881 acagcggatt gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt
    47941 gcagcaacgg gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt
    48001 caagataaac gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag
    48061 agccgccgcc agtagccagc acaataccct gtttctcggt caactcattg atgacctttt
    48121 cttcgcgatc gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc
    48181 cggttcgttt ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta
    48241 actggcgccc aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt
    48301 tctctgccat tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca
    48361 gagagcgccg caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa
    48421 ttatctcaat actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct
    48481 ggtcgctgca agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat
    48541 tagcgtaaaa aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg
    48601 cagcgaagag caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc
    48661 aaatagatga aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg
    48721 gaaaccagtc gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca
    48781 tgacgaaata attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc
    48841 gatttatttt tacgggtaaa aatgccgccc agcgccaacg tttctccgct tttgacctcg
    48901 acctgcgttt cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc
    48961 acctgccccg gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac
    49021 accgtgggcg tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca
    49081 ctttccccgc tggaaacctg atatggaatt tcgctcccct gtttaatgct ggcaggctga
    49141 agatgtgagg ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc
    49201 gcagaaagct caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc
    49261 gttgtcgccg tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca
    49321 gcgtgttgcg catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta
    49381 atggtgacaa tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc
    49441 cactgttcaa gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt
    49501 ttatcgacgg tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct
    49561 cccgcgtcgg cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc
    49621 cgcgcctgct ccgcctcctg gcgggcgata ttgttattct gccaggcaat ggaatgcact
    49681 gagagaatgt tgtcttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt
    49741 gcctgcttcc agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac
    49801 acgaccaggt tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg
    49861 tcatccacca tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc
    49921 aacagtagtg cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg
    49981 tggcggttcg cagtttgtcc cggttcccag cgttagtgcg tctggcgtca gctgtaaaat
    50041 tgtccagccg ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc
    50101 ttttattaca ccgatgatgt gctcgcctcg ccctaccatc ccctgatagc gccactggct
    50161 aagttcgcta atccggcata gatcttccgg cggtttaaaa gggtcacgca taccggttaa
    50221 aaggcacaat gcaatacctg ccaacaacca gcgtttaacc ctcatgcggt gtctccagtt
    50281 gtagcgtgaa caaaagatca tcaccttcca cgcttaacga aaaacggctc acgctgacgt
    50341 tgcgctctgc cagccgtgta aatgccgacg gcactgcttc ccacagcgtt ttcaacgcca
    50401 actcacctcc ctgcgcggat ggatgccagg aaaccagttg cgcgccgggc aactgaaaat
    50461 ccagtggcga aaagggcagc gttttttctt cgctgaaggg agtggtgtca accaggcgat
    50521 acagtgtggc ccactgctgg tgatgacttg cccgtagtcg aattaatgcg tcgcgctctt
    50581 catggtgtgt cgatgacaga aaaatgagcg taactaacat cagcaaccag aatgcccagc
    50641 aaaactggcg gaggcggggt gatgtggcga accaccagtc aaagaacatg ttcatcgcta
    50701 accttccttg ttaactgata ctcaaattgc cagcgtccct gcgcatcctg ctgcgtggct
    50761 ccccgctgat tgagatgaaa agacgcatcc tggcggagtg acgtttctag tgcgtttaac
    50821 gcggtaatgc ttgttgtcag ccccttgatc tccagcgttc cctgctgcca gcttatcgtt
    50881 gtcagccagg cgtgctcggg taaaagcgcc gccagtgctt ccagcgcaga ttgccagtcg
    50941 cgggtaaatt gtcgctggcg ctgccgttgc gaacgctgtt cgcgtaactg ctgttgctcc
    51001 agcaaacgtg gcttcgttat ctgtaagctg cgggcgagtt gttgttccgc ctgaagcaaa
    51061 actgcgtcta tgcgcgcttc ggcgctgcct gtcagacgca gtattagcgt tatcccgacg
    51121 gccagcagca gaggcgcaac gaacatcagc aaccagaaac gcagaaaagc ggtccggcgt
    51181 tgctgtcgcc agggcaaaaa attaattggc gggttcatca gtacgccttc ccaagcgcca
    51241 gccccagcgc gatggcaaag tctccaccag gcggcggtag cggcggctga cgaacagaaa
    51301 cggcctccca ggggtcaaat ccgccttcgc cacatatcgc gacgctgtcg ggatcaacgg
    51361 ataacgctgc cgccagctcc ttcgcgctag tcatccccac cgccagtttg cgcccccagc
    51421 tatagcgcgt cgcccacagc cactgttcgt tatcacgcca ggccagacat tgctgatgag
    51481 aaggtaaaaa aggcaggaat cgctgtaatg cactggcatc cggggtgatc gcactaacat
    51541 gaacacgcaa cctttctgcc agagtaagca gcgttgccag ctctttgctt tgcgcggcag
    51601 tcacgttata ggcgggactg agtgagtctt cgctgtaatc gaaacgcagg gagtccggat
    51661 ccatatccag ctcgcgggcc atcgtccctg acagccaggc cgtttgctcc cgctcaccaa
    51721 gggacatcga cgggcgcgga aatgaccgct gtaatgtgcg actggcggga aacgccaaca
    51781 taatgtgatg acgctgcggc agttcgcgac tccacggtaa caacgtttta gccagctgct
    51841 gcgcatcaac aatccgccca tctttgataa tgtcgttctc cagcggcaac cgccaccagc
    51901 gttgcaaaaa gcattctttt gctccccgca cgatcgcaac cgctaccgct tcttgctgtt
    51961 gtaaatgcaa accaatttgc cagatcttaa atgccattgt gatgctctcc ttatcacccg
    52021 tcactctgac gggtatatca atgcgtctgg cttgccttta tactaccgcg cgtttgttta
    52081 taaactgccc aaatgaaact aaatgggaaa tttccagtga agttcgtaaa gtattttttg
    52141 atccttgcag tctgttgcat tctgctggga gcaggctcga tttatggcct ataccgctac
    52201 atcgagccac aactgccgga tgtggcgaca ttaaaagatg ttcgcctgca aattccgatg
    52261 cagatttaca gcgccgatgg cgagctgatt gctcaatacg gtgagaaacg tcgtattccg
    52321 gttacgttgg atcaaatccc accggagatg gtgaaagcct ttatcgcgac ggaagacagc
    52381 cgcttctacg agcatcacgg cgttgacccg gtggggatct tccgtgcggc aagtgtggcg
    52441 ctgttctccg gccacgcgtc acaaggggcg agtaccatta cccagcagct ggcgagaaat
    52501 ttcttcctca gtccagaacg cacgctgatg cgtaagatta aggaagtctt cctcgcgatt
    52561 cgcattgaac agttgctgac gaaagacgag atcctcgagc tttatctgaa caagatttac
    52621 ctcggttacc gcgcctatgg tgtcggtgct gcggcacaag tctatttcgg taaaacggtc
    52681 gaccaactga cgctgaacga aatggctgtg atcgccgggc tgccgaaagc gccttccacc
    52741 tttaatccgc tctactcaat ggatcgtgcc gtcgcgcggc gtaacgtcgt actgtcgcgg
    52801 atgctggatg aagggtatat cacccaacaa cagttcgatc agacacgcac tgaggcgatt
    52861 aacgctaact atcacgcgcc ggagattgct ttctctgcgc cgtacctgag cgaaatggtg
    52921 cgccaggaga tgtataaccg ttatggcgaa agtgcctatg aagacggtta tcgcatttac
    52981 accaccatca cccgcaaagt gcagcaggcc gcacagcagg cggtacgtaa taacgtgctg
    53041 gactacgaca tgcgccacgg ctatcgcggc ccggcaaatg tgctgtggaa agtgggcgag
    53101 tcggcgtggg ataacaacaa gattaccgat acgctgaagg cgctgccaac ctatggtccg
    53161 ctgctgcctg ccgcagtcac cagcgccaat cctcagcaag cgacggcgat gctggcggac
    53221 gggtcgaccg tcgcattgag tatggaaggc gttcgctggg cgcgtcctta ccgttcggat
    53281 actcagcaag gaccgacgcc gcgtaaagtg accgatgttc tgcaaacggg tcagcaaatc
    53341 tgggttcgtc aggttggcga tgcatggtgg ctggcacaag tgccggaagt gaactcggcg
    53401 ctggtgtcga tcaatccgca aaacggtgcc gttatggcgc tggtcggtgg ctttgatttc
    53461 aatcagagca agtttaaccg cgccacccag gcactgcgtc aggtgggttc caacatcaaa
    53521 ccgttcctct acaccgcggc gatggataaa ggtctgacgc tggcaagtat gttgaacgat
    53581 gtgccaattt ctcgctggga tgcaggtgcc ggttctgact ggcagccgaa gaactcacca
    53641 ccgcagtatg ctggtccaat tcgcttacgt caggggctgg gtcagtcgaa aaacgtggtg
    53701 atggtacgcg caatgcgggc gatgggcgtc gactacgctg cagaatatct gcaacgcttc
    53761 ggcttcccgg cgcaaaacat tgtccacacc gaatcgctgg cgctgggttc agcgtccttc
    53821 accccaatgc aggtggcgcg cggctacgcg gtcatggcga acggtggctt cctggtggac
    53881 ccgtggttta tcagcaaaat tgaaaacgat cagggcggcg tgattttcga agcgaaaccg
    53941 aaagtagcct gcccggaatg cgatattccg gtgatttacg gtgatacgca gaaatcgaac
    54001 gtgctggaaa ataacgatgt tgaagatgtc gctatctccc gcgagcagca gaatgtttct
    54061 gtaccaatgc cgcagctgga gcaggcaaat caggcgttag tggcgaagac tggcgcgcag
    54121 gagtacgcac cgcacgtcat caacactccg ctggcattcc tgattaagag tgctttgaac
    54181 accaatatct ttggtgaacc aggctggcag ggtaccggct ggcgtgcagg tcgtgatttg
    54241 cagcgtcgcg atatcggcgg gaaaaccggt accactaaca gttcgaaaga tgcgtggttc
    54301 tcgggttacg gtccgggcgt tgtgacctcg gtctggattg gctttgatga tcaccgtcgt
    54361 aatctcggtc atacaacggc ttccggagcg attaaagatc agatctcagg ttacgaaggc
    54421 ggtgccaaga gtgcccagcc tgcatgggac gcttatatga aagccgttct tgaaggtgtg
    54481 ccggagcagc cgctgacgcc gcctccgggt attgtgacgg tgaatatcga tcgcagcacc
    54541 gggcagttag ctaatggtgg caacagccgc gaagagtatt tcatcgaagg tacgcagccg
    54601 acacaacagg cggtgcacga ggtgggaacg accattatcg ataatggtga ggcacaggaa
    54661 ttgttctgat taaaaaggcg cttcggcgcc ttttcagttg gctgacaaag tgcatttgtt
    54721 tatgccggat gcgggtgaac gcgttatccg gctaacaaaa tcgtgaaaaa atcaatatat
    54781 tgcaggaaac ccataagcct gataagcgtt gtgcatcagg caaacttcac gcatttacac
    54841 tcgcccctgc cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc
    54901 gttgaagtca gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg
    54961 tagcggctct ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat
    55021 attcattttg ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc
    55081 gttcgcacca aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc
    55141 acccggatca attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta
    55201 ttcgcggatc aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt
    55261 ggttggacgc attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct
    55321 ctcgacggta aacagtcggg aacgggctac agtttcaaca ttcagaatgg tgggtttttg
    55381 taatgatttg ctcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg
    55441 acacgctttc ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatc
    55501 gtcctcaaat caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatattc
    55561 cgccttcaga gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt
    55621 taaactccac gcttgccgat agccaaccgc agaatcatgt attgtgtctg gtgcgactga
    55681 ccacgcctga cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc
    55741 tgctttgctg gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg
    55801 tcagctgccc tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga
    55861 acgtagcgcc gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc
    55921 aacgggagcc agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat
    55981 gatgctgaca cacgctatca cgcgttacgg catctctacc gacgatccga ataaatggcg
    56041 ttactacctc gattcggtag aagtccacct gccccctttc tgggaacaat acatcaacga
    56101 tgagaatacc gttgaactga tttataccga ttcgctgccg ctggttattt cactcaacgg
    56161 tcatacgctg caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc
    56221 cacgcaggcg tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa
    56281 agaaacgcat gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat
    56341 cgtcgcctcc ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg
    56401 gctggcaggc ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc
    56461 ggcaaaatcc tccctgcgag aaattcattg tctgcgcggt acaccccgtc gttggggatt
    56521 gtttggcgaa aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt
    56581 ctatcccgca cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga
    56641 tatctatctc gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt
    56701 aaaaaacttt ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct
    56761 ggtgctgttt atgctgttat tctgggtccc gctggatatg ccgctgaaat tcactctctc
    56821 atggatgaaa ggcgcgcaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg
    56881 cgtgcgggta ggcgatacat tgcgtattag cggtacggga atgtgtaata ttcgcacttc
    56941 cggaacctgg agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat
    57001 ctggaatgac gcccgctcat taccattacc agaatctgaa ctggtcaaca aagcgacggc
    57061 attgactgaa gcagttaatc gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag
    57121 tgcctcatta cgttcagcga ttcaaaaatc cggcatggta ttgcttgatg attttggcga
    57181 cattgtactg aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa
    57241 tgcgctggtc aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc
    57301 cgggaagctc gatggcgtga atgtgttatt acgcccggtg agtgcagaat cgctggataa
    57361 cctggtggca acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc
    57421 actaaacagt ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt
    57481 tgatcagccc tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc
    57541 tttccagaaa ctggcacaaa tgctgatgca tacgccgttt aacgccgaag gtatcgtcac
    57601 aaaaatcttc actgacgcca atggtacgca gcatattggc cttcatccga tcccggatcg
    57661 ttccggactg tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc
    57721 catttacaat ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgga
    57781 gattcaggcc tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct
    57841 tatcgaataa cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc
    57901 tggagatttc cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg
    57961 atatggacgg cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc
    58021 ctgaaacatg gggcgccaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc
    58081 agcaatatca cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc
    58141 aactgggtct ggatatctgt gcgatgacca ccgagatggg accgcgtgcc gtactgcgtg
    58201 aagataccat tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca
    58261 ccaatgcgca tccgcacaac ctggcggtaa aacttgagca taccggtctg gacgcacacc
    58321 ttgatttatt actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc
    58381 atgcggtggc cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg
    58441 aagcgattct cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc
    58501 ctgattccgg gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc
    58561 gcctgatccc ctcgctaatg tgaaggagac gccatgaaag agaaacctgc tgttgaggtt
    58621 cgactggata aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa
    58681 atgattgaag gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc
    58741 gagctgaatg ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag
    58801 gcgattactg aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg
    58861 gaaagtgtag agaaacgcga aaaaatggcg atggcacgta aacttaatgc cttaaccatg
    58921 ccgcacccgg accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac
    58981 ggcgacagtg aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta
    59041 catcgctatc tgtttgaaaa ttttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc
    59101 ctgcaacaga tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa
    59161 ctgctggttg cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta
    59221 cagctgcaag gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa
    59281 atgcgcggtg tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg
    59341 gtcggcaatg gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc
    59401 gtggttggtc tggaaggtga taccctggcg gcctgcctgg aagattactt tatgcgttct
    59461 gaacagctgc cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca
    59521 ggcggtatgt tgttgcaggt aatgcctgcg caaaatgccc agcaggacga ctttgaccac
    59581 ctggcgacgc taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa
    59641 gtgttgtggc gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag
    59701 ttcaaatgca cctgctcgcg tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa
    59761 gaagttgata gcatcctgac ggaagatggc gaaattgaca tgcattgtga ttactgcggt
    59821 aaccactatc tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca
    59881 gatccgcaag ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgacg
    59941 taatggcctg atgtgctgcg cttaccaggc ctgtgaaata aatcgcaaat tattgttggt
    60001 cagatacagc gtcgtatccg ttaaaagagt tgtcacttac ttaagtttat ttccagcgtt
    60061 tcaccatcta ccgtcagatc gcgccacgct ttcggcctcg tccccagttt tccatccttc
    60121 gcagctgtga gtaatttttc tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg
    60181 tcccattctt ttctgtctga gtcaaagcca tagacaatga ctctgtcatc attaaaagca
    60241 aacaggatcc gctccgcctg accatcacta ttcagatctt gctcgaccag gacgcaggcg
    60301 tctttttcaa tacaggtcat cacgttatag cgatcctgga ttaaggccga ccagaatgtc
    60361 gcatccggtt taacagaacc aggggcaatt aacacatttt cggctaatac tttttccgat
    60421 acctgttcct gaagatgttg ctctccatct aacgccatca gcagatcccg cgcgcgtttc
    60481 gggtctttca taaacccggc atcgcttttc agcgactcaa gcgccgcacg tccatagcga
    60541 ccgctctgtt cgagcatgta gatagttacc tggtctggcg tgtttttgcc gctctgataa
    60601 cgcgccatat gactgttcac gctaatacgc atactatcca gcaccggcga attaagaagc
    60661 accagtatca ccaacaccaa taacgaaacc gcaaggttca ctttgccctg aagaacaagg
    60721 ggattttgcc cattacgcca gacgatactg acaaaatacc ccagcgacca caccagtaac
    60781 accagcaccg ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc
    60841 agccataacg cccaggcggc gacgaacaca tacagcggag caaccagcaa agcggtttta
    60901 atcaggcaac gcaacggccc cgtccaggga agtgacgctt tttgcggatc gcggacaata
    60961 gccattaaga tcaattgcaa aaaggccagc gttaacagca acccggcggc ggagatgtgg
    61021 cgagaaatcg cgctcaggcc cgtaaacggc agagtgatga taaacatcag ggttagcaat
    61081 gatactaacg gcagcaaccc cgtggcgatt aacgtgaaca acttttgaat agagtcgatt
    61141 aaacgtgact gtgtgcgcgc gagaatcacc gccagtgcgg taaccagacc taatgtaaga
    61201 tacataaacc agtcggttgc aaaaaagagc gtattgaaaa acttgatgcc aacaagttta
    61261 aacaactcac cccacagtaa aagcaccacc cacgtcaggc cattggcgag aaaaatcacc
    61321 agtaatataa gtacgttatg ccatactgac ttgtaaaagt agctatagcg gctactgcca
    61381 ttgcggatac gaaggctttg ttgtatccac ggtagcaaca acattgccat caacagcaga
    61441 tagcagccaa aatcccaaat cgccttttca gctctccagg ggttcatgcc atcagtttgc
    61501 cacttcagcc aaccgctcat ccctgtcgtg gcaataaaca ccagcgccag ccatccccaa
    61561 aggcgcttct gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa cgcaacggtc
    61621 gcaggcacgc cataaacaat ccaactgtga ttttttccgg caagccaggc gatcaacagg
    61681 tagcaaacca gcccctgaag taatccggta gcaatcatcc cccagcgtgt tgccggtgaa
    61741 agttcaacgt tatccatgtt ggttatccag aatcaaaagg tgggttaatt atcgcatccg
    61801 ggcagtagta ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat
    61861 actttaccga ttgaatttgc atcaatttca ttaaggaatg cgattccact cacaatattc
    61921 ccgccatata aaccaagatt taaccttttg agaacatttt ccacacctaa aatgctattt
    61981 ctgcgataat agcaaccgtt tcgtgacagg aatcacggag ttttttgtca aatatgaatt
    62041 tctccagata cgtaaatcta tgagccttgt cgcggttaac acccccaaaa agactttact
    62101 attcaggcaa tacatattgg ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc
    62161 cgcaagaact cgaggcttat ggtatcagtg acgtacatga tatcgtttac aacccaagct
    62221 acgacctgct gtatcaggaa gagctcgatc cgagcctgac aggttatgag cgcggggtgt
    62281 taactaatct gggtgccgtt gccgtcgata ccgggatctt caccggtcgt tcaccaaaag
    62341 ataagtatat cgtccgtgac gataccactc gcgatacttt ctggtgggca gacaaaggca
    62401 aaggtaagaa cgacaacaaa cccctctctc cggaaacctg gcagcatctg aaaggcctgg
    62461 tgaccaggca gctttccggc aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaatc
    62521 cggatactcg tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg cattttgtca
    62581 aaaacatgtt tattcgcccg agcgatgaag agctggcagg tttcaaacca gactttatcg
    62641 ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga actgggtctg aactccgaaa
    62701 acttcgtggc gtttaacctg accgagcgca tgcagctgat tggcggcacc tggtacggcg
    62761 gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg
    62821 cttctatgca ctgctccgcc aacgttggtg agaaaggcga tgttgcggtg ttcttcggcc
    62881 tttccggcac cggtaaaacc accctttcca ccgacccgaa acgtcgcctg attggtgatg
    62941 acgagcacgg ctgggacgat gacggcgtgt ttaacttcga aggcggctgc tacgcaaaaa
    63001 ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt cgcgatgcgc
    63061 tgctggaaaa cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa
    63121 ccgagaacac ccgcgtttct tatccgatct atcacatcga taacattgtt aagccggttt
    63181 ccaaagcggg ccacgcgact aaggttatct tcctgactgc tgatgctttc ggcgtgttgc
    63241 cgccggtttc tcgcctgact gccgatcaaa cccagtatca cttcctctct ggcttcaccg
    63301 ccaaactggc cggtactgag cgtggcatca ccgaaccgac gccaaccttc tccgcttgct
    63361 tcggcgcggc gttcctgtcg ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta
    63421 tgcaggcggc gggcgcgcag gcttatctgg ttaacactgg ctggaacggc actggcaaac
    63481 gtatctcgat taaagatacc cgcgccatta tcgacgccat cctcaacggt tcgctggata
    63541 atgcagaaac cttcactctg ccgatgttta acctggcgat cccaaccgaa ctgccgggcg
    63601 tagacacgaa gattctcgat ccgcgtaaca cctacgcttc tccggaacag tggcaggaaa
    63661 aagccgaaac cctggcgaaa ctgtttatcg acaacttcga taaatacacc gacacccctg
    63721 cgggtgccgc gctggtagcg gctggtccga aactgtaatg atttgaagct ggagaatatc
    63781 tatccagtat cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt tatttaccct
    63841 tcttttgtcg tgccctgcgc ccgcgttacc ggcactggca gccaggcgcg aatggaaagc
    63901 ccgccccgct cgctggtgcc aagctccagc atcccgttat ggttatccac gatacgctgc
    63961 acaattgcca gccctaatcc cgtgccgcta atggtgcgcg cactgtcgcc gcggacaaac
    64021 ggctggaaca ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc ttccacctgg
    64081 aaccaggcgc gattcggctc cgttccgctg ctgactttga tccagccatt gccataacgg
    64141 gcggcgttga ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg cattttcacg
    64201 tcaatgctgc cggggtaaag cgcggtttca atttcccgct catagccact ttcggcagca
    64261 atcacctcac cgagtactgc attaagatcc gccatttcca tcggcatctc ctgcccggtg
    64321 cgcaggtagt cgataaactg ctcaatgatg gcgttgcact cttcgatatc tttattgatc
    64381 gattctgcca gatagccatc ctgctcgctc atcatctcag tcgccaggcg aatacgcgtc
    64441 agcggcgtgc gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc atccgccagt
    64501 tgcttaacac cagccgccat atggttaaag gcacgggtaa cggaacgcac ctcggacgcg
    64561 ccatactcac gcagcggcgg cggaataatc cctttaccaa cctgcaaggc tgcgtgttcg
    64621 agatcgacca acggtcggtt ctggatacga ataaacagcc acgccccgcc tatcgccaat
    64681 agcataatcg ccagcgtata gcggaacagc ggagagaaat cgccctgatg aatttcggtc
    64741 agcggcacgc gtacccagat attgggcgac agccaggttt tcagccagac gacaggcgaa
    64801 cttttgttga cctcaacgcg cacttccgtc gggccgccca gttgctgtgc catctgatgg
    64861 cttaagaatt catagtgttg cgcccaacgc agacctgcct cttcggcagc ctcgttggag
    64921 tagagagaga tccccagctc acggtagatc tcccgacgga aagcgggagg cacaaccaac
    64981 tgcgtgccgt cctccagttg cagtttgtcg gtcatcaaca tacgcacttc gtacgcgagg
    65041 actttattaa actgctggag gctcggcaaa atcgcgaagt tcagcaccac cagataagtc
    65101 gtcaccaggc tggcgaacag caaggtgacg atgagcaata acgtacgggc aaatgaactt
    65161 cgtggcgaga agcgcaatcg cctcatgctt tagagccgtc cggtacaaag acgtagccca
    65221 ggccccaaac ggtctgaatg taacgcggat gcgctggatc ttcttctacc atgcggcgca
    65281 gacgcgaaat ctgcacgtcg atggagcgtt ccattgcgga atattcacga ccacgggcaa
    65341 ggttcatcag cttatcgcgg gagagcggct cacgcggatg gctgaccagt gccttcagta
    65401 ccgcaaactc accgctggtg agcggcatcg gctcatcttc gcggaacatt tcgcgcgtac
    65461 cgaggttaag tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg
    65521 gcagttcgtt cgcctgacga cgcagcaccg cacgaatacg ggccagcagt tcacgcgggt
    65581 taaacggttt tggaatgtag tcgtcagcgc caatctccag gcctacgata cggtccactt
    65641 cttccccttt cgccgtcacc ataatgatcg gcatcgggtt gctctgacta cgaagacgtc
    65701 ggcaaatcga caagccatct tcaccaggta acattaaatc cagtaccata agatggaaag
    65761 attcacgagt cagcaggcga tccatctgtt ctgcattagc gacgcttcga acctggaagc
    65821 cttgttcggt gagataacgt tccagcagcg cacgcaggcg catgtcgtca tcgaccacca
    65881 gaatcttgta gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg
    65941 tgtattctta aaaaagctca cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa
    66001 attgttacaa agcatattaa acagcagctt aagtatacaa tttattcggc gaaacattat
    66061 tgattctgtt gatatgatca cgttataccc aatgtgcgca ttatcaaaca gacaaaggga
    66121 atcaacgaga tgaaaacgcc cctggttacc cgggaagggt atgaaaaact caaacaagag
    66181 cttaattatc tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca
    66241 agtctgggcg accgcagcga aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa
    66301 atcgaccgtc gcgtgcgcta tctcactaaa tgcatggaaa atctcaaaat cgtcgattac
    66361 tcccctcagc aggaaggcaa ggtctttttt ggcgcatggg tggagattga aaacgacgat
    66421 ggcgtgactc accgtttccg tattgtcggc tacgatgaaa tttttggccg taaagattac
    66481 atctctatcg attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggcg
    66541 gtggtgaata cccctgccgg ggaagcgagc tggtatgtca atgctatcga gtacgtgaaa
    66601 ccgtaaggaa gagtcttaac ctcctgccga tggctggcat ttttgccagc cagtccgtat
    66661 aactatcccc tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc
    66721 tgccgcatta ttgcgggtga aattcaggcg cgcccggaac aggttgacgc tgccgttcgc
    66781 ctgcttgacg aagggaatac cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc
    66841 ggtctggatg acacgcagct gcgtaatctg gaaacgcgtc tgagctatct gcgcgagctg
    66901 gaagagagac gtcaggcgat cctcaaatcc atttccgagc aaggcaaact caccgatgat
    66961 ctggcgaagg ccatcaacgc cacactaagc aaaaccgaac tcgaagatct ctacctgccc
    67021 tacaaaccta aacgccgcac ccgcgggcaa atcgccattg aagcagggct tgagccgttg
    67081 gctgacctgc tgtggagcga tccgtcacac acgccagaag tcgccgctgc acaatatgtt
    67141 gatgcagata aaggcgtggc agataccaaa gccgcgctgg acggcgcgcg ctatatcctg
    67201 atggaacggt ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag
    67261 aacgcgcatc tggtctcaac cgtgatgagc ggtaaagaag aggaaggggc aaaattccgc
    67321 gactatttcg atcatcacga accgctgtcc acggtgcctt ctcaccgcgc gctggcgatg
    67381 ttccgtgggc gtaacgaagg cgtactccag ctttcgctga atgccgatcc gcagttcgaa
    67441 gagccgccaa aagagagcta ttgcgagcaa atcatcatgg atcatctcgg cctgcgcctg
    67501 aacaatgctc cggcggatag ctggcgcaaa ggcgtggtga gctggacctg gcgcatcaag
    67561 gtgctgatgc atctggaaac cgaactgatg ggcaccgtgc gcgaacgcgc ggaagatgaa
    67621 gcaatcaacg tctttgcccg taacctgcac gatctgctga tggcggcccc tgccggactg
    67681 cgtgccacga tgggcctcga tccgggtctg cgtaccgggg taaaagtagc ggtggtcgat
    67741 gccactggca aactggtggc gaccgatacc atttacccgc acaccggaca aacagcaaaa
    67801 gcagcgatga ccgttgctgc cttgtgtgaa aagcataacg ttgaactggt ggcgatcggc
    67861 aacggtacag cttcccgcga aaccgaacgt ttctatctcg acgtgcagaa gcagttcccg
    67921 aaagtgaccg cgcagaaagt gatcgtcagc gaagctggcg cgtcggttta ctcggcttcc
    67981 gagctggcag cgcaggagtt cccggatctc gacgtttcgc tgcgtggtgc ggtatctatc
    68041 gcccgccgtt tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa atctatcggc
    68101 gtaggtcagt atcagcatga cgtcagccag acgcaactgg cccgcaaact ggatgcagta
    68161 gtagaagact gcgtaaacgc cgttggcgtc gatctcaaca ccgcttctgt tccgctatta
    68221 acccgcgtgg cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag
    68281 aacggccagt tcaagaaccg tcagcaactg ctgaaagtca gccgtctggg gccgaaagcc
    68341 ttcgagcagt gcgcgggctt cctgcgcatt aaccacggtg ataacccgct ggacgcctct
    68401 accgttcacc cggaagccta tccggtggtg gaacgcattc tggcagcaac acagcaggca
    68461 ctgaaagatc tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact
    68521 gatgaaaaat tcggtgtgcc gacagtaact gacatcatca aagagctgga aaaaccgggt
    68581 cgcgatccgc gtccggaatt taaaaccgct cagtttgccg atggcgtcga gacaatgaac
    68641 gacctgcaac cgggtatgat cctcgaaggc gctgtcacca acgtcaccaa ctttggcgcg
    68701 tttgtcgata ttggcgtgca tcaggacggc ctggttcaca tctcttcatt gtcgaacaag
    68761 tttgtggaag atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg
    68821 gaagtggatc ttcagcgtaa acgtatcgcc ctgaccatgc gtctggacga gcagcctggc
    68881 gaaaccaacg cccgtcgcgg cggcggtaat gaacgcccgc aaaacaaccg cccggcagcc
    68941 aaaccacgcg gtcgtgaagc gcagcctgcc ggtaatagcg cgatgatgga tgcgctggcg
    69001 gcggcaatgg gcaaaaaacg ttaaacgccc tgattgaccc ctaaacctgc gcgataaggt
    69061 gtccacgccg catctggcaa cgaacgcctt gtctgacata aaagtgccgg agaatatctc
    69121 cggcattttt attccacagc caaactcaca atatattccg gcaacattta tcacttcatt
    69181 aacagctgaa accttaatta aacattagcc agtcctggta attcgctatt cgaattatat
    69241 tttcgctgcg atataacctt gagccacatc aacattgagt cagattatta ttcaaaccaa
    69301 cattcgcaca cattttaagt attgctgata gaaaccattc tcattatcat tgtgctgttg
    69361 attatttaat ctctccttcg ttggcaaatc atctggtctc atgtcgctgt caaacgcccc
    69421 atgaggtagt tatccagtta atgagaaaca agtaggcacc tatgcaatac actccagata
    69481 ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc
    69541 tttctcttgg catgttacct ggctcctctt ttaatgtggt acgcgtcgct ccactcggcg
    69601 accccattca tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct
    69661 tattagaagt ggaagcggtt tcctgttaat acggtgataa caacaatgaa aaaattaacc
    69721 ataggcttaa ttggtaatcc aaattctggc aagacaacct tatttaacca gctcactggc
    69781 gcacgtcagc gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga agggcaattc
    69841 tccaccaccg atcatcaggt cacgctggtg gacctgcccg gcacctattc tctgaccacc
    69901 atctcatcgc agacctcgct cgatgagcaa atcgcctgtc actacatttt gagtggcgac
    69961 gccgacctgc tgattaacgt ggtggatgcg tctaaccttg agcgtaacct gtacctgacg
    70021 ctacaactgc tggaactcgg cattccctgc atcgtggcac tgaacatgct cgacattgcc
    70081 gagaagcaaa atattcgtat tgaaattgat gctctgtcgg cgcgtcttgg ctgcccagtg
    70141 atcccgctgg tttcaacccg tggtcgcggt attgaagcgc tcaagctggc gattgatcgc
    70201 tataaagcta acgagaatgt ggaactggtg cattacgcac agccgctact caacgaagca
    70261 gattcactgg caaaagtgat gccttccgac atcccgctga aacaacgtcg ctggctgggc
    70321 ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat
    70381 ctggatgccg ccctcgcccg tctgcgtaat gagatggacg atccggcgct gcacattgcc
    70441 gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg taagcaacac cctgacggca
    70501 gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg
    70561 ccgattttcc tctttgtgat gtacctgatg ttcctgctgg ctatcaacat cggcggggcg
    70621 ttacagccgc tgtttgacgt cggctccgtg gcgctatttg tgcatggtat tcaatggatt
    70681 ggctacacgc tccacttccc ggactggctg actatcttcc tcgcccaggg cctgggtggc
    70741 ggcattaaca ccgtgctgcc actggtgccg cagattggca tgatgtacct gttcctctcc
    70801 ttccttgagg actccgggta tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag
    70861 gcgctgggct tgccggggaa atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta
    70921 ccgtcggtaa tgggtgcacg tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg
    70981 atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc
    71041 ttcttcgggc agaacggtgc gctggcggtc ttctcgctgt atatgctggg tattgtgatg
    71101 gcggtgctga ctggcctgat gctcaagtac accatcatgc gcggtgaagc gacgccgttt
    71161 gtcatggagt tgccggtcta tcatgtacca cacgttaaaa gcctgattat ccagacctgg
    71221 cagcgtctga aaggcttcgt tctgcgtgct ggtaaagtga tcatcatcgt cagcattttc
    71281 ctgagcgctt tcaacagctt ctcgctgagc gggaaaatcg tcgataacat caacgactcg
    71341 gcgctggcgt ccgtcagccg ggtgatcacc ccggtcttca agccaattgg cgtgcatgaa
    71401 gataactggc aggcaacggt tggcctgttt acaggtgcca tggcgaaaga agtggtagtg
    71461 ggtacgctca acaccctcta caccgcagaa aatattcagg acgaagagtt caatccggca
    71521 gagtttaacc tcggtgaaga gctgttcagt gcgatagatg aaacctggca gagcctgaaa
    71581 gacaccttca gccttagcgt actgatgaac cccattgaag ccagcaaagg cgacggcgaa
    71641 atgggtaccg gggcgatggg cgtgatggat cagaaattcg gtagcgcagc agcagcttac
    71701 agctacctga ttttcgtcct gctgtatgta ccatgtatct cggtgatggg agccatcgcc
    71761 cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt gggggctgaa tatcgcttac
    71821 tcgctggcaa cattgttcta tcaggtcgcc agctacagtc agcacccaac ttacagcctg
    71881 gtgtgcattc tggcggttat cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg
    71941 cgtagccggg tggatatcga actgctggca acccgcaagt cggtaagcag ttgctgcgca
    72001 gccagcacca ccggtgattg ccattaatgg cttcacttat tcaggtgcgc gatttgctgg
    72061 cgttacgggg ccgtatggaa gcggcccaga taagccagac attgaacact ccacagccaa
    72121 tgattaacgc catgctgcaa caactggaaa gtatgggcaa agccgtgcgg attcaggaag
    72181 aacctgacgg ctgcctctct ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc
    72241 gcgagtggtg ggcgctgcgt taaccttact ccatcgcctg ttttggaaag cccggtatgc
    72301 gtctgcatcc gggctttttt gcgtgcggct ttccataaaa atgcaactct tgcagcacgg
    72361 cgtaagttcc tttgaaagca tctcgcaggg atgaaaactc gctaatacac aggtgtggag
    72421 tggcgcgtag agtcgcggca ttcaaacaac aggtgaagga acgccatgag caaaaagcag
    72481 agttccaccc cacacgatgc gctgttcaaa ctctttttac gccaaccgga cacggctcgt
    72541 gattttcttg cgtttcattt accggcaccc attcacgcgc tttgtgatat gaaaaccctc
    72601 aagctggagt cgagcagctt tattgatgac gatctgcgtg aaagctattc cgatgtgctg
    72661 tggtcggtga aaacggaaca aggaccagga tacatctatt gtctgattga acatcaaagc
    72721 acctcaaaca aactgatcgc atttcgcatg atgcgttacg ctattgccgc aatgcaaaat
    72781 caccttgatg ctggatacaa aacgttgccg atggtggtgc cattgttgtt ttaccacggt
    72841 attgaaagcc cctatcccta ttcgctgtgt tggctggatt gtttcgccga tcccaaactg
    72901 gcaaggcagc tttatgcctc cgcatttccg ctgattgatg tcaccgtcat gcctgatgat
    72961 gaaatcatgc agcaccgacg tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa
    73021 cgcgatctga tggggctggt agagcaaatg gcctgcttat taagtagtgg atacgctaat
    73081 gacagacaaa tcaaagggct gtttaattac atactgcaaa ccggcgacgc ggtacgtttt
    73141 aacgatttta tcgacggcgt tgccgaacgt tcaccgaaac acaaggagag tttaatgaca
    73201 attgcggaaa gattgcggca ggagggggaa caatccaaag ccctgcatat agccaaaata
    73261 atgcttgaat ccggagtccc tcttacagac atcatgcgct ttaccggggt gtcagaagaa
    73321 gagttggctg cggcgagcca gtaaatttct gtctcgccat ttcaaaagtt gcctacaccc
    73381 tctgcttcaa cgccaccagc aggtgacaaa actcgaccgg atgcgaaata aacggcgcat
    73441 gggccgcttt ggcgaagata tatgattcgc tgtgaggcca aagtttatcc agcatcggca
    73501 ccactttgcg cggcaccaga ccgtcgagat agccatacaa tcgcaaaaac ggcatggaca
    73561 cgttttgcag cggctgacgg agatcgaccg ttttcaggat ttccagcccg ccattaagca
    73621 cgtcaacctc cggcatcggt aacgccagaa cggttttctt caacgcccgc gcatcctggc
    73681 gcgccgtttc agtccccatg gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat
    73741 catcactgag ttgctgctga aatcccgcca gtacgtccgg ttttatcccc ggccactcgt
    73801 cacgagcact aaaacaaggt gacgacgcca cggtgaccag cgcctgaaca cgctcgggat
    73861 gggttaaaac aatttggctt gccaccagcc cgcccagact ccagcctaac caaatggctt
    73921 tatcaggtgc ctgttgcagc acggcttcgg ccatatcagc aagtgacagc gcaccaaatc
    73981 cccggctacg cccgaagccg ggcaggtcaa caaggtgcag cgtaaaatgc gagctaagtt
    74041 cctcgtcaat gcaacgccac acttcggcat tcagtcccca tccgtgcagc agcacaagat
    74101 gaacattccc ctgacctttg gtctgccacc agatgttatt catccgctat tgttctcttt
    74161 tgacttacaa ggatgaacat atgctaacag taccgggatt atgctggcta tgccgaatgc
    74221 cactggcgtt aggtcattgg gggatttgtt cggtctgttc acgcgccacc cgcacagata
    74281 aaacgttatg cccacaatgt ggattacccg ccacacactc ccatcttccc tgcggtcgct
    74341 gcctgcaaaa accgccgccc tggcaaagac tggtcacggt tgccgactat gcgccgccgt
    74401 taagtccgct tatccaccag cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt
    74461 cacgtctgtt actattggaa gtcttacacg ctcgtcgcac caccgggttg caattgccgg
    74521 atcgcatcat cagcgttccg ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga
    74581 gcgatttgct gtgtcagccg ttatcacgct ggttgcattg ccaatgggat agcgaagccg
    74641 tcacacgtac acgggccact gcgacccagc attttctcag tgcccggctg cgcaagcgca
    74701 acctgaaaaa tgcctttcgt cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg
    74761 atgatgtcgt taccaccgga agtaccgtcg cagagattgc gcagttgctt ttacgcaatg
    74821 gtgcggcgac tgtccaggtc tggtgccttt gtcgaacctt gtagagcctc gatgatgggc
    74881 gtattataac caactaaaat agtcaactat taggccatta ctatgatccg tatttccgat
    74941 gctgcacaag cgcactttgc caaactgctg gcaaatcagg aagaagggac acaaatccgc
    75001 gtatttgtga ttaaccctgg cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg
    75061 gatgccgtgg aagccaccga cactgccctg aaatttgacc tgctgaccgc gtatgttgat
    75121 gagttaagcg caccatacct ggaagatgca gagatcgact ttgttaccga ccagttgggt
    75181 tctcagttaa cgctgaaagc cccgaacgcc aaaatgcgta aagtggcaga cgatgcaccg
    75241 ctgatggagc gcgtggagta tatgctgcag tcgcagatca acccacagct tgctggtcac
    75301 ggtggtcgcg tttcgctgat ggaaatcacc gaagacggtt acgccattct gcaatttggc
    75361 ggcggctgta acggttgttc catggtcgat gtgacgctga aagaagggat cgagaagcag
    75421 ctgctgaacg aattcccgga gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc
    75481 gaacactcct actactaagt tatcccctct tttgtagatt gcccgatgcg acgctaaagc
    75541 gtcatttcgg gcctacgttg aacatgtgcc agacgttggc acagcattgc tttggcaaat
    75601 ccctcccttc gtttccccgc gataatatga ccaacctctc ataatttaaa tttaccccgc
    75661 tctggtgatt ctcaaacgcc agatgttacc cgtatcattc acatgggtac caaacatact
    75721 cctgacatct gactacaata attagtttta gtgggtatca gtcgtggtgc cgcaatatct
    75781 ctgttcccga ttgggataat tagagtttgt cgtcagaaaa ttgacgttac ccataacaaa
    75841 tgaaaggcca ggtaaatcat gccattagtc attgttgcta tcggtgtaat cttgttgttg
    75901 ctcctgatga tccgcttcaa aatgaacggc ttcatcgctc tcgtcctcgt ggcgcttgct
    75961 gttggattaa tgcaaggaat gccgctggat aaagttattg gctccatcaa agccggtgtc
    76021 ggcgggacgc tcggtagcct tgccctgatc atgggttttg gcgcaatgct gggcaaaatg
    76081 ctggcagact gcggtggcgc acaacgtatc gccaccacgc tgattgccaa atttggtaaa
    76141 aaacacatcc agtgggcggt ggtactgacc ggttttaccg ttggttttgc cctgttctat
    76201 gaagtgggct ttgtgctgat gctgccgctg gtgtttacca tcgcggcttc tgcgaatatt
    76261 ccactgttgt atgttggtgt accaatggca gctgcactgt ctgtgaccca cggcttcctg
    76321 ccaccgcatc cgggtccgac tgcgattgcc accattttca atgccgatat gggtaaaacc
    76381 ctgctgtacg gtactattct ggcaatcccg accgtgattc tcgccggtcc ggtttacgct
    76441 cgcgtgttga aaggtatcga taagccaatt ccggaaggtc tctacagcgc gaaaaccttc
    76501 agcgaagaag agatgccgag ctttggcgtc agcgtctgga cctctctggt gccggtagta
    76561 ctgatggcga tgcgtgcgat tgccgaaatg atcctgccga aaggtcacgc tttcctgccg
    76621 gtagcggagt tcctcggtga cccggtaatg gctacgctga ttgccgtgct gattgccatg
    76681 ttcacctttg gtctgaaccg tggtcgttca atggatcaga ttaacgacac gctggtttct
    76741 tccatcaaaa tcattgcgat gatgctgttg atcatcggtg gtggcggtgc gttcaagcag
    76801 gtgctggtag acagcggcgt ggacaaatac attgcttcca tgatgcacga aaccaacatt
    76861 tctccgctgc tgatggcctg gtcgattgct gccgtactgc gtatcgcgct gggttccgca
    76921 accgttgcgg caatcactgc gggtggtatc gcggcaccgc tgattgcaac gacgggtgtt
    76981 agcccggagc tgatggttat tgcggtgggt tccggtagtg tgattttctc tcatgtgaac
    77041 gatccgggct tctggctgtt caaagagtac tttaacctga ctatcggcga gaccatcaaa
    77101 tcctggtcga tgctggaaac cattatctcg gtgtgcggtc tggtaggctg tctgctgctg
    77161 aatatggtga tttgataaca ctgactgccg gatgcatcgc atccggcatc aaccgcactc
    77221 tacttcttct tcgctgcagc tctgcgccgt ctgtccaaat ccttcagcaa cttgttcacg
    77281 ccatcatcgg caaacatcga ctcaagcgtt gcggaaagct tgcgtcgcca gtttttatac
    77341 tggtaactgg tgccaggaat attcaccggt tcggccatat ccagccagtc ttccggctgt
    77401 agtcctaaca gagcactgtt actgtcggca atgtagcgct gcaaaccacg gttcagcgtc
    77461 ggcgtcatcg acatcaacga tgccttatgc ccggcacgtt ttggcagaca accatattta
    77521 tgcagtgcat ccagcagccc ttgcttcgcc agttcgcgat cctgatacag accgcgcagt
    77581 accacttcat ccggatacag ccccagggtt ttgcccagcg ttagatcccc gctctcccag
    77641 taaccgcgta gcgtaggcag gtcatgtgtc gccgcaaccg ccatcgactg ctccggatac
    77701 gctttcggtg cacggaacgt cttctcgtgg tcgttttcga aatagagcac tttgtaggag
    77761 tacacaccgc tgctgcgcag cttaccgaca atctctaccg gtacggtacc gagatcttca
    77821 ccaatcacca tacagcgatg acgtttactt tcgagtgcca gaatcgagag cagatcatcc
    77881 accggatagt gaacatacgc gccctgatct gccgtctcgc catacggtat ccaccacaaa
    77941 cgcagcatcg acatcacatg gtcaattcgt aatgcgccgc agttttgcat attggcacgc
    78001 aacagctcga taaacggttc ataggcacgc gcggtgatga tatgcgggtc cattggcggt
    78061 aatccccagt tctgccccaa cgggccgagg atatccggcg gcgcgccaac cgacgctttc
    78121 aggcaatata gttcacggtc acaccaggtt tccgccccac cttccgctac gccaaccgcc
    78181 agatcacgat acaagccaat cggcatttca tagccctggc ttatctccca gcaggcggca
    78241 aactggctgt aagccagcca ctgcaaccag agataaaaat cgacgtcatc acgatgttct
    78301 tcgcagaact gacgcacttc tggtgaatcc acgttctgat acatctctgg ccatgcaggc
    78361 cagccccagc gcatttcgtc ctctttcact tgctgggcat gtagcgcatc aaaggctgcc
    78421 tgccagaaca ggctgtcgcc ctgctctgca acaaactggc gaaacgcggt catctgctca
    78481 tcatcacgtt gcgcgaaacc tttccacgcc attcgtaatg ctgtcatttt tagggcggta
    78541 accgtggagt aatcgaccca gtcggcatcg cgcgcctgtt gcagcgtctg ttgcgtggtc
    78601 ggcaactgcc accaggcctg agcctcttcg ctaagatgga aatcttcaac ggcgttaacg
    78661 tcgatataaa tcacattcag ccaacggcga gaagacgggc tgtatgggct ggcactctcc
    78721 ggatttgccg gatagagcgc atgaatcggg ttcaggccga tgaacgaccc gccacgtttt
    78781 gccacatcca ccagcatcgc tttgagatcg ccaaaatccc caatacccca gtttttttcc
    78841 gatcgcagcg tataaagctg aacgcaggca ccccacagct tttgtttatt cagcaacgcc
    78901 tgcggttcgt aacagcgttt cggggcgaca atcacccggc aatgcgcgcg ctggtcgtcc
    78961 tgggtgagtg tcagcgtgtg ataaccttcc ggcagcttcg tcggtagatt gaacgctttg
    79021 ccccccgtta catggccttt gtactgtgtt ccttcttcgg tggtcagcag ccagctatat
    79081 tcgccgctgc cctccaccac catcggcatt tttttgccgc tggtataaac catgacattc
    79141 gggactggcg ttaccgccac tttcgtggcg gtacgttgat gcatcgcgtc aagcaaacgc
    79201 cgtttggttt cggcgctaat cgactgcggt ttaccgtggg cattgatgta attggggcta
    79261 atccccgccg ccagcgcggc attatccaga cgtttgcttt ccatcgagct tccttagcgt
    79321 tttgcctgcc agatacgagc ctgataatcg cgaatagagc gatccgagct aaacataccg
    79381 cagcgggcgg tattgaggat cgccgcgcga gtccaggcct cctggtcgcg gtacagcaca
    79441 tccacctgct tttgtgcctc aacataggct gcgaaatccg ccatcaccag atacggatcg
    79501 ccgccttgtt tgccgatact gtgcagcatc tggtcgaagg catgcttatc gccgtcgctg
    79561 tatttaccgc tttccagctc tttcaatact gcgtccagca ccttatcttt cttccgccat
    79621 ttcaccgggt cgtagccttt ggccagaata gccttcactt gttccacggt atgaccaaaa
    79681 ataaagatat tttcttcacc gactttctcg gcgatttcaa cgttcgcacc atccagcgta
    79741 ccgacagtaa gcgcaccatt gagcgccagt ttcatattgc cggtaccgga agcttcttta
    79801 cctgcagtcg aaatttgttc ggagatatcc gccgccggga tcattttttc cgccgccgaa
    79861 acgcaataat ccggcaggaa caccaccttc aacttatcgc caaccagtgg atcgttgttg
    79921 atcacgtcag ccactttgtt gatcgcaaag ataatattct tcgccaggta gtagcccggt
    79981 gccgctttcg cgccgaagag gaagacgcgc ggtacgcgat cagcctgcgg gttttcacga
    80041 atttctttgt acaacgccag aatatgcagc agattcaggt gctggcgttt gtactcatgc
    80101 agacgtttga tctgaatatc gaaaatcgcc tgtggattga tctcaatacc ggtacgaact
    80161 ttcacaaact ccgccagacg gactttattc gcctgcttga tctcgcgata ttgctgacgg
    80221 aatttcgcat catcagcgaa tttttccaga ttgatcagct gatcgagatc gttagcccac
    80281 tctttttgca gtgatttatc caacagagcc gccagtgcag ggttgcactg tttgatccag
    80341 cgacgtgggg taataccgtt ggtgacgtta tggaatttgt tcggccatag ctggtgatat
    80401 tccgggaaca gatctttcac caccagatcc gagtgcagcg ccgcaacacc gttcaccgcg
    80461 aaaccgccaa ccacacacag gttcgccata tgcacttgtt tatcgtgcac caccgccagt
    80521 ttggcccaca ctttttcatc gcccggccag gttttctcca ccagcgtttt aaagcgagta
    80581 ttaatttcgt taataatctg catatggcgc ggcagtaagc ctttcaccag tttcacatcc
    80641 cagcgttcca gcgcttctgg catcagggta tggttggtgt aagcgaaagt tttgctggta
    80701 atggcccagg cgtcatccca gctcatctgg tgctcatcga tcagcacgcg cagcagttct
    80761 ggaatcgcga tagttgggtg ggtatcgttc agctgaataa cttcgtaatc cgccagttcg
    80821 tgcagtttac gccccgccag atgatggcga cgcaaaatat ccgctaccga acaggcacac
    80881 tggaagtact gctgcatcaa gcgcagcttt ttaccggcag tatggttgtc gtttggatag
    80941 agaactttgg tcagtttttc cgcattgatg ccctgctgtt cggcacgcag gaaatcaccg
    81001 tcgttaaatt tagtcagatc aaacggatgc gcgtgcgtcg cctgccacag acgcagcggc
    81061 tgcgccacgc cattacgata gccgacaacg gggagatccc acgcttgacc ggtaatggta
    81121 aactccggct cccagcgtcc gtctttcgtc actttaccgc caatccctac ctgcacatcc
    81181 agtgcttcgt tgtggcggaa ccacgggtag ttactgcgat gccagtcatc cggcgcttca
    81241 acctgtttgc catcgacaaa agactggcgg aacaaaccat attgatagtt cagaccgtaa
    81301 cccgtcgcag actgaccgac agttgccatt gagtcgagga agcacgccgc cagacgtccc
    81361 agaccaccgt tacccagcgc cgggtcgatc tcttcttcca gcaggtccgt cagattgatg
    81421 tcataagcct tcaacgaatc ctgtacatcc tgataccagc cgagattcaa caggttgttg
    81481 cccgtcaggc gaccaatcaa aaactccatt gagatgtagt taacatgtcg ctgattcgcc
    81541 accggcttgg cgaatggctg agcacgcagc atttcggcca gtgcttcact cactgctagc
    81601 caccactggc gaggagtcat ttcagccgca gaatttaagc cataacgctg ccactgacgt
    81661 gaaagcgctt cctgaaattg cttatcgtta aaaataggtt gtgacatagg agttccactt
    81721 ttcttagatt ttcaacacaa cgttatcgct agtttgccag gctcgatgtt gaccttcctc
    81781 atcctgcggg ggattaggca gggaggagtt gcggggatga gcaaggaaat gtgatctcaa
    81841 ccacttaaag ctagtgcaaa ccacaggatt agcagcaaat caatgcaata cagcgcagaa
    81901 aatctgtatc taagtgcaaa aaatggccgt tgcgtatttt caaaaagcgg aaggtaactc
    81961 tataaattaa gtaaaggagt gaaacagttt cataagtaaa atat
//
LOCUS       DAZGOB010000005        79265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGOB010000005 DAZGOB010000000
VERSION     DAZGOB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.005"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(156..974)
                     /gene="cof"
                     /locus_tag="ACHLIF_000322"
     CDS             complement(156..974)
                     /gene="cof"
                     /locus_tag="ACHLIF_000322"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO9604398.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            1074..2774
                     /gene="ybaE"
                     /locus_tag="ACHLIF_000323"
     CDS             1074..2774
                     /gene="ybaE"
                     /locus_tag="ACHLIF_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO9604399.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2839..3534
                     /gene="queC"
                     /locus_tag="ACHLIF_000324"
     CDS             2839..3534
                     /gene="queC"
                     /locus_tag="ACHLIF_000324"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO9604400.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(3586..3984)
                     /gene="fadM"
                     /locus_tag="ACHLIF_000325"
     CDS             complement(3586..3984)
                     /gene="fadM"
                     /locus_tag="ACHLIF_000325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO9604401.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(4078..4449)
                     /gene="ybaV"
                     /locus_tag="ACHLIF_000326"
     CDS             complement(4078..4449)
                     /gene="ybaV"
                     /locus_tag="ACHLIF_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO9604402.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(4600..6471)
                     /gene="ppiD"
                     /locus_tag="ACHLIF_000327"
     CDS             complement(4600..6471)
                     /gene="ppiD"
                     /locus_tag="ACHLIF_000327"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO9604403.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKVVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(6663..6953)
                     /gene="hupB"
                     /locus_tag="ACHLIF_000328"
     CDS             complement(6663..6953)
                     /gene="hupB"
                     /locus_tag="ACHLIF_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO9604404.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(7144..9498)
                     /gene="lon"
                     /locus_tag="ACHLIF_000329"
     CDS             complement(7144..9498)
                     /gene="lon"
                     /locus_tag="ACHLIF_000329"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO9604405.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(9686..10960)
                     /gene="clpX"
                     /locus_tag="ACHLIF_000330"
     CDS             complement(9686..10960)
                     /gene="clpX"
                     /locus_tag="ACHLIF_000330"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO9604406.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(11086..11709)
                     /gene="clpP"
                     /locus_tag="ACHLIF_000331"
     CDS             complement(11086..11709)
                     /gene="clpP"
                     /locus_tag="ACHLIF_000331"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO9604407.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(11956..13254)
                     /gene="tig"
                     /locus_tag="ACHLIF_000332"
     CDS             complement(11956..13254)
                     /gene="tig"
                     /locus_tag="ACHLIF_000332"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO9604408.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(13598..13915)
                     /gene="bolA"
                     /locus_tag="ACHLIF_000333"
     CDS             complement(13598..13915)
                     /gene="bolA"
                     /locus_tag="ACHLIF_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO9604409.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            14220..14798
                     /gene="yajG"
                     /locus_tag="ACHLIF_000334"
     CDS             14220..14798
                     /gene="yajG"
                     /locus_tag="ACHLIF_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9604410.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            14842..16317
                     /gene="ampG"
                     /locus_tag="ACHLIF_000335"
     CDS             14842..16317
                     /gene="ampG"
                     /locus_tag="ACHLIF_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO9604411.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTGVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            16777..17724
                     /gene="cyoA"
                     /locus_tag="ACHLIF_000336"
     CDS             16777..17724
                     /gene="cyoA"
                     /locus_tag="ACHLIF_000336"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO9604412.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            17746..19737
                     /gene="cyoB"
                     /locus_tag="ACHLIF_000337"
     CDS             17746..19737
                     /gene="cyoB"
                     /locus_tag="ACHLIF_000337"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO9604413.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            19727..20341
                     /gene="cyoC"
                     /locus_tag="ACHLIF_000338"
     CDS             19727..20341
                     /gene="cyoC"
                     /locus_tag="ACHLIF_000338"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO9604414.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            20341..20670
                     /gene="cyoD"
                     /locus_tag="ACHLIF_000339"
     CDS             20341..20670
                     /gene="cyoD"
                     /locus_tag="ACHLIF_000339"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO9604415.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            20682..21572
                     /gene="cyoE"
                     /locus_tag="ACHLIF_000340"
     CDS             20682..21572
                     /gene="cyoE"
                     /locus_tag="ACHLIF_000340"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO9604416.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            21757..22068
                     /locus_tag="ACHLIF_000341"
     CDS             21757..22068
                     /locus_tag="ACHLIF_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO9604417.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            22052..22594
                     /locus_tag="ACHLIF_000342"
     CDS             22052..22594
                     /locus_tag="ACHLIF_000342"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9604418.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(22650..23585)
                     /locus_tag="ACHLIF_000343"
     CDS             complement(22650..23585)
                     /locus_tag="ACHLIF_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO9604419.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELVAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLARSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            23993..25357
                     /gene="yajR"
                     /locus_tag="ACHLIF_000344"
     CDS             23993..25357
                     /gene="yajR"
                     /locus_tag="ACHLIF_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9604420.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(25485..25976)
                     /gene="yajQ"
                     /locus_tag="ACHLIF_000345"
     CDS             complement(25485..25976)
                     /gene="yajQ"
                     /locus_tag="ACHLIF_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO9604421.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            26144..27055
                     /gene="panE"
                     /locus_tag="ACHLIF_000346"
     CDS             26144..27055
                     /gene="panE"
                     /locus_tag="ACHLIF_000346"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO9604422.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            27018..27608
                     /gene="yajL"
                     /locus_tag="ACHLIF_000347"
     CDS             27018..27608
                     /gene="yajL"
                     /locus_tag="ACHLIF_000347"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO9604423.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(27662..29110)
                     /gene="thiI"
                     /locus_tag="ACHLIF_000348"
     CDS             complement(27662..29110)
                     /gene="thiI"
                     /locus_tag="ACHLIF_000348"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO9604424.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            29316..29558
                     /gene="xseB"
                     /locus_tag="ACHLIF_000349"
     CDS             29316..29558
                     /gene="xseB"
                     /locus_tag="ACHLIF_000349"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO9604425.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            29558..30457
                     /gene="ispA"
                     /locus_tag="ACHLIF_000350"
     CDS             29558..30457
                     /gene="ispA"
                     /locus_tag="ACHLIF_000350"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO9604426.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            30482..32344
                     /gene="dxs"
                     /locus_tag="ACHLIF_000351"
     CDS             30482..32344
                     /gene="dxs"
                     /locus_tag="ACHLIF_000351"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO9604427.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            32524..33498
                     /gene="yajO"
                     /locus_tag="ACHLIF_000352"
     CDS             32524..33498
                     /gene="yajO"
                     /locus_tag="ACHLIF_000352"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO9604428.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEESEMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(33552..34070)
                     /gene="pgpA"
                     /locus_tag="ACHLIF_000353"
     CDS             complement(33552..34070)
                     /gene="pgpA"
                     /locus_tag="ACHLIF_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO9604429.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(34048..35025)
                     /gene="thiL"
                     /locus_tag="ACHLIF_000354"
     CDS             complement(34048..35025)
                     /gene="thiL"
                     /locus_tag="ACHLIF_000354"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO9604430.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(35103..35522)
                     /gene="nusB"
                     /locus_tag="ACHLIF_000355"
     CDS             complement(35103..35522)
                     /gene="nusB"
                     /locus_tag="ACHLIF_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO9604431.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(35542..36012)
                     /gene="ribE"
                     /locus_tag="ACHLIF_000356"
     CDS             complement(35542..36012)
                     /gene="ribE"
                     /locus_tag="ACHLIF_000356"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO9604432.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(36101..37204)
                     /gene="ribD"
                     /locus_tag="ACHLIF_000357"
     CDS             complement(36101..37204)
                     /gene="ribD"
                     /locus_tag="ACHLIF_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO9604433.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(37208..37657)
                     /gene="nrdR"
                     /locus_tag="ACHLIF_000358"
     CDS             complement(37208..37657)
                     /gene="nrdR"
                     /locus_tag="ACHLIF_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO9604434.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            37808..38347
                     /gene="yajI"
                     /locus_tag="ACHLIF_000359"
     CDS             37808..38347
                     /gene="yajI"
                     /locus_tag="ACHLIF_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO9604435.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            38646..39530
                     /gene="tsx"
                     /locus_tag="ACHLIF_000360"
     CDS             38646..39530
                     /gene="tsx"
                     /locus_tag="ACHLIF_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO9604436.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(39707..40054)
                     /gene="yajD"
                     /locus_tag="ACHLIF_000361"
     CDS             complement(39707..40054)
                     /gene="yajD"
                     /locus_tag="ACHLIF_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO9604437.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(40183..41154)
                     /gene="secF"
                     /locus_tag="ACHLIF_000362"
     CDS             complement(40183..41154)
                     /gene="secF"
                     /locus_tag="ACHLIF_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO9604438.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(41165..43012)
                     /gene="secD"
                     /locus_tag="ACHLIF_000363"
     CDS             complement(41165..43012)
                     /gene="secD"
                     /locus_tag="ACHLIF_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO9604439.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(43040..43372)
                     /gene="yajC"
                     /locus_tag="ACHLIF_000364"
     CDS             complement(43040..43372)
                     /gene="yajC"
                     /locus_tag="ACHLIF_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO9604440.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(43395..44522)
                     /gene="tgt"
                     /locus_tag="ACHLIF_000365"
     CDS             complement(43395..44522)
                     /gene="tgt"
                     /locus_tag="ACHLIF_000365"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO9604441.1"
                     /translation="MKFELDTTDGRARRGRLVFDCGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(44578..45648)
                     /gene="queA"
                     /locus_tag="ACHLIF_000366"
     CDS             complement(44578..45648)
                     /gene="queA"
                     /locus_tag="ACHLIF_000366"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO9604442.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            45741..46322
                     /gene="acpH"
                     /locus_tag="ACHLIF_000367"
     CDS             45741..46322
                     /gene="acpH"
                     /locus_tag="ACHLIF_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO9604443.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(46327..48144)
                     /gene="malZ"
                     /locus_tag="ACHLIF_000368"
     CDS             complement(46327..48144)
                     /gene="malZ"
                     /locus_tag="ACHLIF_000368"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO9604444.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFDGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(48300..49673)
                     /gene="proY"
                     /locus_tag="ACHLIF_000369"
     CDS             complement(48300..49673)
                     /gene="proY"
                     /locus_tag="ACHLIF_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO9604445.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(49749..51068)
                     /gene="brnQ"
                     /locus_tag="ACHLIF_000370"
     CDS             complement(49749..51068)
                     /gene="brnQ"
                     /locus_tag="ACHLIF_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO9604446.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(51475..52770)
                     /gene="phoR"
                     /locus_tag="ACHLIF_000371"
     CDS             complement(51475..52770)
                     /gene="phoR"
                     /locus_tag="ACHLIF_000371"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO9604447.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWLRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(52828..53517)
                     /gene="phoB"
                     /locus_tag="ACHLIF_000372"
     CDS             complement(52828..53517)
                     /gene="phoB"
                     /locus_tag="ACHLIF_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO9604448.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            53707..54909
                     /gene="sbcD"
                     /locus_tag="ACHLIF_000373"
     CDS             53707..54909
                     /gene="sbcD"
                     /locus_tag="ACHLIF_000373"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO9604449.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            54906..58049
                     /gene="sbcC"
                     /locus_tag="ACHLIF_000374"
     CDS             54906..58049
                     /gene="sbcC"
                     /locus_tag="ACHLIF_000374"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO9604450.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQRIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASETQEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            58175..59359
                     /gene="araJ"
                     /locus_tag="ACHLIF_000375"
     CDS             58175..59359
                     /gene="araJ"
                     /locus_tag="ACHLIF_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO9604451.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIITPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCCGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(59502..60410)
                     /gene="mak"
                     /locus_tag="ACHLIF_000376"
     CDS             complement(59502..60410)
                     /gene="mak"
                     /locus_tag="ACHLIF_000376"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO9604452.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            60535..61446
                     /gene="rdgC"
                     /locus_tag="ACHLIF_000377"
     CDS             60535..61446
                     /gene="rdgC"
                     /locus_tag="ACHLIF_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO9604453.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(61524..61607)
                     /locus_tag="ACHLIF_000378"
     CDS             complement(61524..61607)
                     /locus_tag="ACHLIF_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9604454.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(61604..61945)
                     /locus_tag="ACHLIF_000379"
                     /pseudo
     CDS             complement(61604..61945)
                     /locus_tag="ACHLIF_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(62093..62377)
                     /gene="ppnP"
                     /locus_tag="ACHLIF_000380"
     CDS             complement(62093..62377)
                     /gene="ppnP"
                     /locus_tag="ACHLIF_000380"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO9604455.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(62449..63126)
                     /gene="aroM"
                     /locus_tag="ACHLIF_000381"
     CDS             complement(62449..63126)
                     /gene="aroM"
                     /locus_tag="ACHLIF_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO9604456.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(63384..63575)
                     /gene="yaiA"
                     /locus_tag="ACHLIF_000382"
     CDS             complement(63384..63575)
                     /gene="yaiA"
                     /locus_tag="ACHLIF_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO9604457.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(63625..64149)
                     /gene="aroL"
                     /locus_tag="ACHLIF_000383"
     CDS             complement(63625..64149)
                     /gene="aroL"
                     /locus_tag="ACHLIF_000383"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO9604458.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(64332..64790)
                     /gene="yaiI"
                     /locus_tag="ACHLIF_000384"
     CDS             complement(64332..64790)
                     /gene="yaiI"
                     /locus_tag="ACHLIF_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO9604459.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            64910..65719
                     /gene="proC"
                     /locus_tag="ACHLIF_000385"
     CDS             64910..65719
                     /gene="proC"
                     /locus_tag="ACHLIF_000385"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO9604460.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(65736..66851)
                     /gene="adrA"
                     /locus_tag="ACHLIF_000386"
                     /gene_synonym="dgcC"
     CDS             complement(65736..66851)
                     /gene="adrA"
                     /locus_tag="ACHLIF_000386"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO9604461.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(66953..67273)
                     /gene="psiF"
                     /locus_tag="ACHLIF_000387"
     CDS             complement(66953..67273)
                     /gene="psiF"
                     /locus_tag="ACHLIF_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO9604462.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(67392..68807)
                     /gene="phoA"
                     /locus_tag="ACHLIF_000388"
     CDS             complement(67392..68807)
                     /gene="phoA"
                     /locus_tag="ACHLIF_000388"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO9604463.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(68908..69168)
                     /gene="iraP"
                     /locus_tag="ACHLIF_000389"
     CDS             complement(68908..69168)
                     /gene="iraP"
                     /locus_tag="ACHLIF_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO9604464.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(69350..69553)
                     /locus_tag="ACHLIF_000390"
     CDS             complement(69350..69553)
                     /locus_tag="ACHLIF_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9604465.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            69631..70725
                     /gene="ddlA"
                     /locus_tag="ACHLIF_000391"
     CDS             69631..70725
                     /gene="ddlA"
                     /locus_tag="ACHLIF_000391"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO9604466.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(70749..70961)
                     /gene="yaiZ"
                     /locus_tag="ACHLIF_000392"
     CDS             complement(70749..70961)
                     /gene="yaiZ"
                     /locus_tag="ACHLIF_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO9604467.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            71221..71529
                     /gene="yaiY"
                     /locus_tag="ACHLIF_000393"
     CDS             71221..71529
                     /gene="yaiY"
                     /locus_tag="ACHLIF_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO9604468.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(71588..72682)
                     /gene="yaiW"
                     /locus_tag="ACHLIF_000394"
     CDS             complement(71588..72682)
                     /gene="yaiW"
                     /locus_tag="ACHLIF_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO9604469.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(72695..73915)
                     /gene="sbmA"
                     /locus_tag="ACHLIF_000395"
     CDS             complement(72695..73915)
                     /gene="sbmA"
                     /locus_tag="ACHLIF_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO9604470.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            74267..75424
                     /gene="ampH"
                     /locus_tag="ACHLIF_000396"
     CDS             74267..75424
                     /gene="ampH"
                     /locus_tag="ACHLIF_000396"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO9604471.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMVPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(75425..76048)
                     /gene="iprA"
                     /locus_tag="ACHLIF_000397"
     CDS             complement(75425..76048)
                     /gene="iprA"
                     /locus_tag="ACHLIF_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO9604472.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(76136..78833)
                     /locus_tag="ACHLIF_000398"
                     /pseudo
     CDS             complement(76136..78833)
                     /locus_tag="ACHLIF_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(78863..>79265)
                     /locus_tag="ACHLIF_000399"
     CDS             complement(78863..>79265)
                     /locus_tag="ACHLIF_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO9604473.1"
                     /translation="SRAVIGWSMSPRMTAQLACDALQMALWWRKRPRNVIVHTDRGGQ
                     YCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATV
                     FNYIECDYNRWRRHSLCGGLSPEQFENKNLA"
BASE COUNT        19313 a        20917 c        19518 g        19517 t
ORIGIN      
        1 catagccctt ccacagagaa tttttttctc ttttaattct attttaaagg tgaaatcacg
       61 acaatttatt ctgtgcggtt taaacaccac ggcacaaaat gacaaattca acgcaaaaaa
      121 aaccgggcaa ttgcccggtg ctggaaggga tctcgttatt cgggggaata aggtagatgt
      181 ggatagtcca gccagtgcgt caaatagtga gagacagcct gatttcggca atgtccaatc
      241 accggtaaat gcgggagctc cgcgcgcagt tgcggcatcg cattgcccat aataaacccg
      301 ctaccgacgc tgcctaacat ttcgcgatcg ttcatcgcat caccaaaggc catgcaatcg
      361 cgcaacgata aacctaaatg ttgggtcagc accgtcaatg cagcgccttt attgcagccc
      421 accggcagca cttcgaggca atccgtggcg gaaaaacaca aatgtgcacg ctcgcctaat
      481 gcttcgtata gctggatctg caagcgtgta agatcgtcgt gaccgccaca gaagcagatc
      541 ttggtgactc tgccgagtgg catttttttg acatcgatta tctgataacg aaaaccgcta
      601 tagacaaatg cctgcaacaa cgcagggatc tctttcccgg taaaccaacc gtcgtcattg
      661 aagatatgca tgctggctcg ggtatcccat tgctgataca gcaccagctc cgcgacatcc
      721 gcaggtaaat catcacgatg taaaagttca ccttccagag aatgcacgcg cgttccgttg
      781 ccggtaatca aatatgcatc cagcgatagc gccccgagaa tatgctgcat ctccagcgca
      841 tgacgccccg tggcaaaagt gagggtaatg tcgcgttcac gcagtcgcgc caaagtagag
      901 agggttttct cacctaaatg atggtcaggc atcaataaag tgccatccat atcaaatgct
      961 gccagacgag ccatttcttt ctccactctg tgacacggtt aatggtgatt gagtatcacc
     1021 tgatatatac ggaagtaata gtgaatagtt aaataatatt gttccgggtt tatatgcgat
     1081 tgctcaaccg tcttaaccag tatcaacgtc tgtggcaacc ttcagccgga aagccgcaaa
     1141 ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag cgaacgccat gttcgtacgc
     1201 tgttgcgtca ggcacaggag gcgggatggc tggagtggca ggcgcagtca ggacgcggaa
     1261 agcgcggaca attacgcttt ctggtcacgc cggaatcgct acgcaatgcg atgatggaac
     1321 aggcactgga aaccggaaag cagcaagatg tgctggagct ggcgcaactg gccccaggtg
     1381 agctgcgcac tctgttacag ccgtttatgg gcggacaatg gcaaaacgat acacccacgt
     1441 tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc aggctttttg cccggccgtg
     1501 ccgagcagca tctcgccggg cagatatttt ccggcctgac ccgcttcgat aataatactc
     1561 agcgcccgat tggcgattta gcgcatcact gggaaacctc tactgacggg ttacgctggg
     1621 acttttatct tcgttcaacc ctacactggc ataacggcga tgcagtaaaa gcctcacact
     1681 tacaccagcg attattgatg ctgttacaac tgccagcact ggatcaatta tttattagcg
     1741 tgaagcatat tgaagtcacc catccgcagt gtctgacctt ctttttacat cgccccgatt
     1801 actggcttgc gcaccggctg gcgagctatt gcagccatct ggcgcatccg caattcccac
     1861 tgatcggcac tggtcctttt cgcttaacac aattcacagc ggagctggtg cgcctggaga
     1921 gccatgatta ttaccatttg cgtcatccgc tgcttaaagc ggttgagtac tggataactc
     1981 cgccgctttt cgaaaaagat ttgggaaaca gttgtcggca tcccgtgcaa atcaccatcg
     2041 gcaaaccgga ggagctgcaa cgggtcagcc aggtcagtag tggcatcagt ttaggttttt
     2101 gctatttaac gttgcgcaaa agtccccgac tctccctctg gcaggcgcga aaagtgatct
     2161 ccattattca tcaatccggt ttattacaaa cgttagaagt cggagaaaac ctgatcaccg
     2221 ccagtcatgc attactgcca ggctggacta ttccgtactg gcaagtaccg gatgaagtca
     2281 aactaccgaa aaccttgacg ctggtttatc acctaccgat agaacttcat accatggcag
     2341 aacgcctaca ggcgacactg gcagcggaag gctgtgaact cacaattatt tttcataacg
     2401 caaaaaactg ggacgacacg accctactgg cacacgcaga cctcatgatg ggcgacagat
     2461 taattggcga agcaccggaa tatactctgg agcaatggct acgttgcgat ccactatggc
     2521 cacatgtttt cgacgctcca gcatatgcac atctgcaatc gacgctggac gcggtgcaag
     2581 taatgcctga tgaggaaaac cgatttaatg ccctgaaagc ggtttttagc cagttaatgg
     2641 cagacgcgac gctgacgccg ctgttcaact atcactatcg cattagtgcc cctcccggcg
     2701 tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt taccgaagcc tggcttcccg
     2761 cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc gttaccataa cgcttttatt
     2821 gttcaagcag gattatctat gaaacgtgct gtcgttgtgt tcagtggagg ccaggattcc
     2881 accacctgtc tggtgcaggc attacaacaa tatgatgaag tccattgcgt gacgttcgat
     2941 tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg aactggcgct gaaactggga
     3001 gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg agctggcggt cagcagcctg
     3061 acacgtgaca gcattccggt gcctgattat gaacctgaag ccgatggcat cccgaatacg
     3121 tttgtccctg ggcgtaatat tttgttcctg acgctggcgg caatatatgc gtatcaggta
     3181 aaagcagaag ccgtaattac cggcgtctgt gaaacggatt tctccggcta cccggattgc
     3241 cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt tgggcatggc gaaagatatt
     3301 cgttttgaaa cgccgctgat gtggattgat aaagcggaaa cctgggcgct ggcagattat
     3361 tacggcaaac tggatttagt ccgtaacgaa acgttgacct gctataacgg ctttaaaggc
     3421 gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca acggtttgaa tcattatctg
     3481 gccgataaac cgacggtgat ggcagcgatg aagcagaaaa ccgggttgag gtaattattc
     3541 cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa aacgtttact taaccatctg
     3601 ctccagcttt tcgcgcaatt ccccttccag agctaatgct ttctgcgttt taagatcaat
     3661 acaaacaaac gtaataagcg catccgctac cacctgccct tccggctcca gtgtaatgac
     3721 ctggcttaag atgccgcttt taccgtttaa ttgctgcaac tgactggtaa tagttaacag
     3781 gtcgcttaat accgctgggc gacgatagtt aatattgata ttgaccacga cgaaggcaat
     3841 gttatgggcc gtcatccact gaaaactgtc gctattttcc aacccatccc agcgggcttc
     3901 ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg taaacgtcga gatgatatcc
     3961 acgaactttg atttgtgttt gcatagcgca ataaccttac gttgttgtta taagtacaga
     4021 ggtcataact ggtatgacct cttcagtctg gcaaaaaatt gccactatgc aaattaatta
     4081 cagggttaat accgccagat tacgttccac cagcgaattg cccatccccg gcacctgctt
     4141 tagatcctcc accgttttaa acggaccgta ctcttcgcga taactgacaa tcgcctgcgc
     4201 tttcttcagg ccaacgccat tcatcgcgcg ggctagctct tccgcgctgg cattattaat
     4261 gctgacccgg gtgccttctt cgtcactggc tttcgccggt actgctgctt tactttgtgc
     4321 tgcaggagct tccgctttgg tttctaccgc cgtcggtttc gccacagaag ctgccgccag
     4381 cgcgctatga gacattccgg cacaggccag ggacagggta atgagcagtg ctttaattcc
     4441 gtgtttcata ctgttttctc cttgtttgtt aacagtggaa tcaccttaac gggagtgaaa
     4501 acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg cgaaagcgac cttttcttgt
     4561 tacagctgca acgcattttt ttgcgaggcg gcttcaggat tattgctgtt ccagcgcatc
     4621 gccaattttg attttcgcct ctttacgcag gttactcatc agagcttcaa agacgatttg
     4681 tgcgttgttc tgggtgatac cctgcaccat cgcttttttc tgatcttccg gcattgaacc
     4741 ttgtttcact tcatccagcg ccagcagaac cacattacct tgcatatcgg tcgccatacc
     4801 gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac gccgcctggc taatcgggtc
     4861 acgaccggaa cggcttaaga ttttcggctc gccaaatttc agaccggcag cctgcatagc
     4921 ttccgcacct ttgccggctt tcaaatcaac cagcagtttc tcagcatcca ctttcgcctg
     4981 ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt tcctgaacat ctgccaacgg
     5041 tttcaccgct tccggtttgt gctcgctgat gcgcagcacg aatgcgcggt cgccgtctac
     5101 ggtgatgatg tcagagttga tgcccggcgc gccgttttca cctaccagac cgccgttaaa
     5161 gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc aggttatctt tgctaaacca
     5221 acccgtctga gtggctttaa cgccggcagc ttgctctgca ccggccagag actccgtgtc
     5281 gttgcttgcc gcatcgctca ctttctgctg cagcgcatag tacgcatcga gggctttttc
     5341 gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct aacgatttca ctttcgctgg
     5401 ctgaatgtcg tccagacgta caatcaggaa accgaccgaa gatttgatga caccagacag
     5461 ttgccctttt tctttcagac cagcattttt cagttcgtcg gggatagtgg catcttctaa
     5521 ccaacccata tcgccgccgt tacgggcaga gataatatcg gcagattttt ctttggctaa
     5581 tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc actttcgctt catcttcagt
     5641 tttggtctgg atgatgctgt agcgggtacg ctgcggctgg gtgaattgat cctgatgctg
     5701 atcgtagtag ctctggatat ccgcatcgct aaccggttgc tgcatcgttg cggcatccag
     5761 cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa ttgtttttgt tttgttcgta
     5821 gtagctggca atctcctgtt cggtcacagg ctgcttcgcc gccagcgcgt taacatcgat
     5881 agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc gccagctcgt cggtttcacc
     5941 tttcagcata aaatcggtac cggcaacacc gttaatcagc tgttgggtag tgagctggtt
     6001 acgcagcgcc tgggcgtact gatcggcggt catccccatc tggttgagga taccgttata
     6061 gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg gtcgcgaaaa tcgcctgttt
     6121 aacctgctca tcactgatac ccagtttcag ctcacgagcg tactgatcca acagcgcctc
     6181 gtcgatcaga cgattcagca cctgttgacg cagggttttc atatagcctt cgttcgctgc
     6241 cagctcggag tattgatcgc ccagctgttg ctgcatgcga ttacgctcgc tgttaaaggc
     6301 gttctcgaac tgcccacggc tgatttcctg gtcattcact tttgcggcgt agttattgcc
     6361 tccgccaatc aggtaaccac tcacgccggt caatatgaac gacacgataa tgataccgaa
     6421 aataatcttg agcacgagac tgtttgcagc cgtgcgtaag ctgtccatca tggtgtaaca
     6481 acactccgct gtagatgact gtgtacctca cgcaacgtat cgatgcgcgc aaaggcctat
     6541 tgtgacaaga aacgggggca attgtcagcc cacaagacac agaaagtcac cgaatacaaa
     6601 taaaaaaggc acatcagtag atgcgccctt gaacttcgtc acatccccac tggggacaac
     6661 gcttagttta ccgcgtcttt cagtgcttta cctgcacgga agctcggtac tttagcagca
     6721 gcgatggtga tctctttacc ggtctgcggg ttgcggccag tacgggcagc acgctcttta
     6781 acggcaaaag taccaaaacc taccagtgct acatcatccc cttctttcag agattcagtt
     6841 acggaagcaa taatagcatc taacgcacgg ccagccgcag ctttagagat atcagcccct
     6901 gcagcaatct tgtcgatcaa ttgagattta ttcactcttc tcttcctctt tataatttat
     6961 atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt atatcaggcc agccatcccc
     7021 ttacaacacc ccttaataag ggcaagcccg atccgccatc taacttagcg agacaaaaaa
     7081 aggctggcaa gcccgaatta gcctgccagc cctgttttta ttagtgcatt ttgcgcgagg
     7141 tcactatttt gcagtcacaa cctgcatgcc agacggttca ttttgcagcg ccagagtcag
     7201 aacttcctca atgcgcttca caggatgaat gtccagatcg gcaattacgt tgtcaggaat
     7261 ctcttccaga tcgcgtttat tttcgaacgg aattagcact gttttaatcc cgccgcgatg
     7321 cgctgccagg agtttttctt tcaaaccacc gatcggcagt acctgaccac gcagagtgat
     7381 ctcaccggtc attgccacat cggcacgaac cgggttaccg gtcaggcaag aaaccagcgc
     7441 ggtgcacata gcaataccgg cactcggacc atctttcggc gtcgcacctt ccggtacgtg
     7501 gacgtggatg tcacgttttt cgtaaaaatc agggttgatc cccagttttt ccgcacgcgc
     7561 acgaaccacc gttaacgccg cctgaataga ctcctgcatc acttcgccga gcgaaccggt
     7621 ataggtcagt ttgcctttac ccggaacaca tgcggtttca atggtcagca agtcaccgcc
     7681 cacttccgtc cacgccagac cggttacctg accgacacgg ttttcgttat cagcgcgacc
     7741 atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta tcgccgttaa tttcgatatg
     7801 ttttaatgac ttatcgagca gtaactgctt aaccgctttg cggcacagtt tggagatttc
     7861 acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa cgaataatgc cgataatggc
     7921 gctatcgtcg acggtcagct cacctttttt cagtgcatta cgttcaatct gcttcggcag
     7981 caggtgacgt ttggcgatgt tcagtttttc atcttcggta taaccggaga ggcgaatcac
     8041 ttccatacga tccagcagcg gtgccggaat gttcatggag ttcgacgtcg cgacaaacat
     8101 cacgtcgctg agatcgtaat ccacttccag gtagtggtcg ctgaacgcta cgttctgctc
     8161 tggatccagc acttcaagca gtgcagaggc cggatcgcct cgcatgtcag aagacatttt
     8221 gtcgatctca tcgagcagga acagcgggtt tttcacgccc actttcgcca ttttctggat
     8281 cagtttaccc ggcatagaac cgatgtaagt acggcggtga ccacggattt ccgcttcatc
     8341 acgcacgccg cccagcgcca tacggacata tttacgcccg gtggctttgg caatggactg
     8401 accaagagag gttttaccta cccccggcgg ccctaccagg cagaggatcg gtcccttgat
     8461 tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg cgatctttca cgcgctccag
     8521 accataatgg tcggtatcaa ggatttcctg cgcctgacgc aggtcttttt tgaccttgct
     8581 gcgcgcattc cacggtacct gtaccatcca gtcgatataa ccacgcacta cggtcgcttc
     8641 tgccgacatc ggagacatca ttttcagctt ctgcaactct gcttccgctt tctcttttgc
     8701 ctctttcggc attttcgccg cgtcgatttt gcgcttcagg gcttcgtttt cgtccggcgc
     8761 gtcgtccatt tcaccgagtt ctttctgaat agctttcatt tgctcgttca gatagtactc
     8821 acgctgggat ttctccatct gctttttaac gcggttgcga atgcgtttct caacctgcag
     8881 cagatcgatt tccgattcca tcattgccat cagatattcc agacgttcgt taacgtcgga
     8941 catctccaga acggactgtt tgtcagccag tttcagcggc atatgtgcag caatggtatc
     9001 cgccagacgc gccggatcgt cgatgctatt cagcgacgtc agcacttctg gtgggatttt
     9061 tttgttcagc ttgatgtagc cttcgaactg gctgattgca gtacgcacca gcacttcctg
     9121 ttcccgctca tcaatggtcg gcgactccag atactccgcc ttcgcagaaa agtgttcgcc
     9181 attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc tcgaccagca ctttgacggt
     9241 gccgtcaggc agtttcagca tctgcaatat agaggccacg gtcccgacgg tgaaaagatc
     9301 gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg accagcataa tttttttatc
     9361 atggtccatc gccgcttcca gacaacggat agatttttcc cgcccgacaa ataaggggat
     9421 gaccatgtgc ggataaacca ccacatcgcg cagcggcaat acggggattt caatgcgttc
     9481 agaacgctca ggattcatag agctctctct tagtttaatt tccgccaggt aatcagatga
     9541 cacgactgtg cttcacgcca tctattaaca tgtacgtcag tatatgggga tgtttccccc
     9601 acattcaacg ccgagaatag aggaaaaatt aaaggggaga taaaatcccc cctttttggt
     9661 taactaattg tatgggaatg gttaattatt caccagatgc ctgttgcgct tccggcttac
     9721 cataaatcag caacggtttg ctttgaccat caattaccga ctcgtcgata accacttttt
     9781 cgacatcttc catggacggc agatcgtaca tggtatcgag cagtgcggct tctacgatgg
     9841 aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt cttagcgata gcatccagcg
     9901 cctcgtcacg gaattccaga tccacgcctt ccagattaaa cagcgcctga tactgcttgg
     9961 tcagggcgtt tttcggctct ttgaggatct gaatcagagc ttcttcgctc agttcattca
    10021 acgttgcgac aaccggcaga cgaccaataa actcagggat aagaccaaac ttgatcagat
    10081 cttccggttc aacctgcgcc agcagctcgc cttcgcttgc tttgtcggac ttcgctttta
    10141 ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg gtgggaaatc actttatcca
    10201 gaccggcaaa cgcaccgcca caaataaaca ggatcttaga ggtatcaacc tgcaagaatt
    10261 cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc agctaccgta ccttcgatca
    10321 gtttcaacag tgcctgctgt acgccttcac cggaaacgtc tcgggtaatg gacgggttgt
    10381 ctgacttacg agaaatcttg tcgatctcat cgatgtagac aatcccgcgc tgcgctttct
    10441 gtacgtcgta atcgcacttc tgcaacagct tctgaatgat gttttcaacg tcttcgccca
    10501 cataaccggc ttcggtcagc gtggttgcgt cggccatggt gaacgggacg tccagcaggc
    10561 gcgccagcgt ttcagccagc agcgttttac cggaaccggt cggaccgatc agcagaatgt
    10621 tacttttgcc caactcgaca ccattgctgg tatcgccgtt gcgcagacgt ttgtaatggt
    10681 tgtataccgc gaccgccagc acttttttcg cctgttcctg gccgataacg taatcgtcca
    10741 ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg ttcacgatgc ggtgcaactt
    10801 ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac acattcgtcg cagatataca
    10861 cggatggacc ggcaatcagc ttgcgcactt catgctggct tttgccgcaa aaagagcaat
    10921 acagcaattt gcctgagcca tctttgcgtt tatctgtcat gggtcaaaac ctcttctttg
    10981 ttctttgtgc cgcacacgac gacgcaaatg ccattctcag gcgcaagccg cttacagcgt
    11041 tgtgccgccc tggataagta tagcggcaca gttgcgcctc tggcatcaat tacgatgggt
    11101 cagaatcgaa tcgaccagac cgtattccac cgcttcaggg gcggaaagga agcgatcgcg
    11161 ctcggtatca cgttcaatct gttctaatga ttgacccgta tgaagcgcca taagttcatt
    11221 catgcgccct ttaactttca gaatttcacg ggcatgaatt tcgatatcgg tcgcctggcc
    11281 ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa ttcggcaggc aaaaacgttt
    11341 accttttgcc cctgcggtca acaagaaagc gcccatcgag gccgcctggc ccatacagat
    11401 ggtgctgaca tcaggcttga taaactgcat ggtgtcatag atagacatcc cggcagtgat
    11461 caccccgcct ggggagttaa tgtacagata gatatctttt tctgggtttt ccgcttccag
    11521 gaacagcatc tgcgccacaa tcaggttagc catgtggtct tcaacctggc cagtcagaaa
    11581 aatgacgcgt tccttaagta gacgagaata gatatcaaaa gagcgctcac cgcgtgaggt
    11641 ctgttcaatg accatcggca ccagcgccat atggggtgca aagttatctc gttcgccgct
    11701 gtatgacatt tccgtctcct ggataaaatt gaaaaaacct gctgtaccga ttgtaacctt
    11761 atggacggat tatcagctag tccctgtgtt atgggtacgg catcaccata tttcaagcat
    11821 aacaatcttt tgttgttacg ctaacaccga aagaggcttt tcgcacggtt ccatgcaaaa
    11881 ttcatgacaa aaaaaaagcc caccacctgg cggtgacggg cctttgtgcg aatttagcgc
    11941 gttatgctgc gtaaattacg cctgctggtt catcagctcg ttgaaagtgg tttctttttc
    12001 agtcactttc gctttcgcca gtacagcttc aacagcctgt tcttccagag caacattgcg
    12061 catgttgtcc atcagttctt tgtttttgct gtagaactcg ataacttctt tcggatcttc
    12121 gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc tcttcgtcag ctttcagctc
    12181 gttggtgcgg ataacttcgc ccagcagcag gccaacaact acgcggcgtt tagcctgttc
    12241 ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg ttgccaccga aacgctgtgc
    12301 agcctggcga cgcagaacgt cgatttcgct gtcgatcagc gcagccggta cgtcgatgtc
    12361 gttagctttt accagacctt cgatcgcctg agacttaacg cggttacgga tggcgctctt
    12421 cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga ccttctacgg aaccatcttc
    12481 aacgccgaaa cgtttgatga attctgcagt cagttccggc agttcacgct cttcaacttt
    12541 cttcaggttg atagcgaatt tcgctgcttt acctttcagg ttttctgcgt ggtattcttc
    12601 cgggaaggtc acgtcgatgg tgaactcttc gccagctttg tggcctttga taccgtcttc
    12661 aaagcccggg atcatacgac cctggcccat cgccagtacg aaatcagacg ctttaccgcc
    12721 ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg gttacgcggt cttctgcttc
    12781 aacagcgccg tctttttctt tccaggtcgc ctgctgttta cgcagagtat ccagcatgcc
    12841 gtcaacgtca gcgtcggtca cttcaacgat cggtttttca acttcgatcg cttccagacc
    12901 ctgcagttca acttccggat aaacttcaaa ctctacagag taagtgaagt cttcacccag
    12961 cttgtattcg cccggaacat aagtcggtgc gccagccgga ttgatttttt ctttaatgat
    13021 ggcgtcaatg aagttacggc tcatcagatc acccagaacg tcctggcgta cagacgcgcc
    13081 ataacgctga gcaacgatat tcattggcac tttgcctttg cggaagccgt caatacgtac
    13141 ttttttcgca acgttgacca gctcgctttt aacagcggtc tcgatgctgt cagcagcgat
    13201 agtaatcgtt acacggcggc caaggccttg agtggtttca actgaaactt gcatcttgtt
    13261 acctcaaaaa atcacagtgc tcggtcaact cttaccccgc aagcacaggt tgttggcttc
    13321 gcggaaccgg gatgttctta taatcaatca cattccctgt taccagaatc atcccgaaag
    13381 cattcaaata ggacgcggca ttatagcggc atcactttta tgagtcgaga acggttattg
    13441 cgcgctgtcg cgtatttttt cacaattccc gggcaatttt cgtctgaatg ctgggcaaaa
    13501 agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt ttttcataaa atctttagca
    13561 acatactgga aaagcgccga cagttgcaaa tgcgttttta cgcgatgctt cctgctccac
    13621 gacagggagg agaggcaaag acggtgtcct gcaacccttc ccactcctta atagtgtaag
    13681 tatgcagagc cagcgcatga acggtagtag agagttcctc cgctaaagta ctgtaaatca
    13741 ttcgatgacg attcagaaaa cgttcacccg taaaacgatc gctgaccagc acaactttaa
    13801 aatggctttc agagccggct gggacattgt gacgatagct ttcatccact acttcgagga
    13861 atacgggttg gaacgccgcc cttaattttt cttctatccg ctcacgtatc atcatgaaat
    13921 atctcctccg acaacgctga gatgtcatcc atccctttaa atactagccg cttttaccgt
    13981 ttccattgca gcttaacaac aaatatttag gtttcgtcct ggtttttccg tcaagcgtat
    14041 gaagtttact gatttaaaga ttgaaacaca ggcctcgcat cagcgtttct tttaccaggg
    14101 gtgattattt cgtcaacatg atgaatttac atgccttacc cacttcccct cgccgttggc
    14161 aatgttatga tggcgggaat ttttaccaaa cacgcttaag agtctctgag agcccgaaca
    14221 tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat gcttgcagga tgcgcaaaac
    14281 cgccaacaac tattgaagtt tccccgacga ttacactgcc acagcaggat ccaagcctga
    14341 tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac cgatcaggcg ctggcaaaag
    14401 tcacccgcga taatcaaatc gttaccctga ccgcctcccg cgatctgcgt ttcctgctgc
    14461 aagaagtgct ggaaaaacag atgaccgcgc gtggttacat ggttggtccg aatggcccgg
    14521 ttaatctgca aatcatcgtt agccaactgt atgctgacgt gtcccagggc aacgtgcgct
    14581 acaacatcgc taccaaagcg gatatcgcca tcatcgctac cgcgcagaat ggcaacaaaa
    14641 tgaccaaaaa ctatcgtgcc agctacaatg ttgaaggtgc gttccaggcc tccaacaaaa
    14701 atatcgccga tgcggttaac agtgtgctga ccgataccat cgctgatatg tctcaggaca
    14761 ccagcattca cgaattcatc aagcagaacg cgcgttaatt tctgccctct ggcccggtgc
    14821 aagccgggcc tgtagacgcc catgtccagt caatatttac gtatttttca acagccgcgt
    14881 tcagccatat tgctgatcct gggttttgct tccgggctac cgctcgccct gacatccggc
    14941 accttacagg cctggatgac ggtcgagaat attgatctca aaaccattgg tttcttctct
    15001 ctggtaggcc aggcttacgt ttttaaattc ctctggtcac cgctgatgga ccgctacacg
    15061 cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca cgcaaatcct gttattagtc
    15121 gccattgcgg cgatgggttt tctcgaacct ggcacccaac tccgctggat ggcggcactg
    15181 gcggtggtga tcgctttttg ctcggcctcc caggatattg tcttcgatgc gtggaaaacc
    15241 gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa tcagcgtgct gggttaccgt
    15301 ttagggatgc tggtttccgg cggcttagcc ctgtggctgg cagataaatg gctgggctgg
    15361 cagggcatgt actggttgat ggcggcgctg ttgatccctt gtattatcgc gacgttgctt
    15421 gcaccagaac cgaccgacac cattcctgtg ccaaaaacgc tggaacaagc ggttgttgca
    15481 cctctgcgag atttctttgg tcgcaataat gcctggctta ttttgcttct tatcgtgctg
    15541 tataagctgg gcgacgcatt cgccatgagc ctgacaacca cgtttttgat tcgcggcgtc
    15601 gggtttgatg cgggtgaagt aggcgtggtt aacaaaacgc ttggcttact ggcgaccatt
    15661 gtgggcgcat tgtacggtgg gattttgatg cagcgcctgt cgctgttccg ggcgctgctg
    15721 attttcggca ttttacaagg tgcgtctaac gctggttact ggctgctgtc gattactgat
    15781 aagcatctct acagcatggg cgcagccgtc tttttcgaaa acctctgtgg cgggatgggc
    15841 acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata agtcattttc cgcaactcaa
    15901 tttgccctgc tctcagcgct ttccgctgta ggacgagttt atgtcggccc cgtggcgggt
    15961 tggtttgttg aagcacacgg ctggtcgaca ttctatctat tctccgtcgc cgctgccgta
    16021 ccggggctta ttttgctgct ggtttgccgc cagacgcttg aatatacagg agtaaatgac
    16081 aactttatct cccgtaccga atatccggca ggttatgcct ttgccatgtg gacactggcg
    16141 gcgggcgtca gcctgttggc cgtgtggtta ctgctgttga cgatggacgc gctggatttg
    16201 acgcacttct ctttcctgcc tgctctgctg gaagtcgggg ttttagtcgc cctttctggc
    16261 gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa aaacgcatct gacgtaatct
    16321 gtaaatatta tttagctgta gtaatcactc gccgtaatta ttacggctaa ataaacatca
    16381 gtatcgctta ataatatttg ttcttttgtg cggcttagcg tttggaaatt attgacgcaa
    16441 tttatgaatt ctattttttc ttacacgatt cagctaatga gtctttattt tcccccaccc
    16501 aattgtcacc ataatgataa ttatttgtta aacaattgtt ttattccaga ttggttatac
    16561 caattgcccg tccaaactcc ttctgcactc ccctttcgtt ataacgccct tttgcaacag
    16621 cttcttaaaa tcaacttgat atgttttgca acatatgtga cctgacagcc aaatccaagt
    16681 aacaggaatt taatcatgtt tacagtaatg taaccttccc gtaaaatgcc cacacacttt
    16741 aaacgccacc agatcccgtg gaattgaggt cgttaaatga gactcaggaa atacaataaa
    16801 agtttgggat ggttgtcatt atttgcaggc actgtattgc tcagtggctg taattctgcg
    16861 ctgttagatc ccaaaggaca gattggtctg gagcaacgtt cactgatact gacggcattt
    16921 ggcctgatgt tgattgtcgt tattcccgca atcttgatgg ctgttggttt cgcctggaag
    16981 tatcgtgcga gcaataaaga tgctaagtac agcccgaact ggtcacactc caataaagtg
    17041 gaagctgtgg tctggacggt acctatctta atcatcatct tccttgcggt actgacctgg
    17101 aaaaccactc acgctcttga gcctagcaag ccgctggcac acgacgagaa gcccattacc
    17161 atcgaagtgg tttccatgga ctggaaatgg ttcttcatct acccggaaca gggcattgct
    17221 accgtgaatg aaatcgcttt cccggcgaac actccggtgt acttcaaagt gacctccaac
    17281 tccgtgatga actccttctt cattccgcgt ctgggtagcc agatttatgc catggccggt
    17341 atgcagactc gcctgcatct gatcgccaac gaacccggta cttatgacgg tatctccgcc
    17401 agctacagcg ggccgggctt ctcaggcatg aagttcaaag ctattgcaac accggatcgc
    17461 gccgaattcg accaatgggt cgcaaaagcg aaacagtcgc cgaacaccat gtctgacatg
    17521 gctgcgttcg aaaaactggc cgcgcctagc gaatacaacc aggtggaata tttctccaac
    17581 gtgaaaccag acttgtttgc cgatgtgatt aacaagttta tggctcacgg taagagcatg
    17641 gacatgaccc agccagaagg tgagcacagt gcacacgaag gtatggaagg catggacatg
    17701 agccacgcgg aatccgccca ttaaaggggt tgaggaagaa taaagatgtt cggaaaatta
    17761 tcacttgatg cagtcccgtt ccatgaacct atcgtcatgg ttacgatcgc tggcattatt
    17821 ttgggaggtc tggcgctcgt tggcctgatc acttacttcg gtaagtggac ctacctgtgg
    17881 aaagagtggc tgacctccgt cgaccataaa cgcctcggta tcatgtatat catcgtggcg
    17941 attgtgatgt tgctgcgtgg ttttgctgac gccattatga tgcgtagcca gcaggctctt
    18001 gcctcggcgg gcgaagcggg cttcctgcca cctcaccact acgatcagat cttcaccgcg
    18061 cacggcgtga ttatgatctt cttcgtggcg atgcctttcg ttatcggtct gatgaacctg
    18121 gtggttccgc tgcagatcgg cgcgcgtgac gttgcgttcc cgttcctcaa caacttaagc
    18181 ttctggttta ctgttgttgg tgtgattctg gttaacgttt ctctcggcgt gggcgaattt
    18241 gcgcagaccg gctggctggc ctatccaccg ctatcgggaa tagagtacag tccgggagtc
    18301 ggtgtcgatt actggatatg gagtctccag ctatccggta taggtacgac gcttaccggt
    18361 atcaacttct tcgttaccat tctgaagatg cgcgcaccgg gcatgaccat gttcaagatg
    18421 ccagtattta cctgggcatc actgtgcgcg aacgtactga ttattgcttc cttcccaatt
    18481 ctgacggtta ccgtcgcgtt gttgaccctg gatcgctatc tgggcaccca tttctttacc
    18541 aacgatatgg gtggcaacat gatgatgtac atcaacctga tttgggcctg gggccacccg
    18601 gaagtttaca tcctgatcct gcctgttttc ggtgtgttct ccgaaattgc ggcaaccttc
    18661 tcgcgtaaac gtctgtttgg ttatacctcg ctggtatggg caaccgtctg tatcaccgtg
    18721 ctgtcgttca tcgtttggct gcaccacttc tttacgatgg gtgcgggcgc gaacgtaaac
    18781 gccttctttg gtatcaccac aatgattatc gccatcccga ccggggtgaa gatcttcaac
    18841 tggctgttca ccatgtatca gggccgcatc gtgttccatt ctgcgatgct gtggaccatc
    18901 ggttttatcg tcactttctc ggtgggcggg atgactggcg tgctgctggc agtaccgggc
    18961 gcggacttcg ttctgcataa cagcctgttc ctgattgcgc acttccataa cgtgatcatc
    19021 ggcggcgtgg tcttcggctg cttcgcaggg atgacctact ggtggcctaa agccttcggt
    19081 ttcaaactga atgaaacctg gggtaaacgc gcgttctggt tctggatcat cggcttcttc
    19141 gttgccttta tgccgctgta tgcgctgggc ttcatgggca tgacccgtcg tttgagccag
    19201 cagattgacc cgcagttcca caccatgctg atgattgcag ctagcggtgc agtactgatt
    19261 gcgctgggta ttctctgcct cgttattcag atgtacgttt ctattcgcga ccgcgaccag
    19321 aaccgtgacc tgactggcga cccgtggggt ggccgtacgc tggagtgggc aacctcttcc
    19381 ccgcctccgt tctataactt tgccgttgtg ccgcacgttc acgagcgtga tgcattctgg
    19441 gaaatgaaag agaaaggcga agcgtataaa aagcctgacc actatgaaga aattcatatg
    19501 ccgaaaaaca gcggtgcagg tatcgtcatt gcagctttct ccaccatctt cggtttcgcc
    19561 atgatctggc atatctggtg gctggcgatt gttggcttcg caggcatgat catcacctgg
    19621 atcgtgaaaa gcttcgacga ggacgtggat tactacgtgc cggtggcaga aatcgaaaaa
    19681 ctggaaaacc agcatttcga tgagattact aaggcagggc tgaaaaatgg caactgatac
    19741 tttgacgcac gcgactgccc acgcgcacga acacgggcac cacgatgcag gcggaaccaa
    19801 aatcttcgga ttttggatct acctgatgag cgactgcatt ctgttctcaa tcttgtttgc
    19861 tacctatgcc gttctggtga acggcaccgc aggcggcccg acaggtaagg acattttcga
    19921 actgccgttc gttctggttg aaactttctt gctgttgttc agctccatca cctacggcat
    19981 ggcggctatc gccatgtaca aaaacaacaa aagccaggtt atctcctggc tggcgttgac
    20041 ctggttgttt ggtgccggat ttatcgggat ggaaatctac gaattccatc acctgattgt
    20101 taacggcatg ggtccggatc gcagcggctt cctgtcagcg ttctttgcgc tggtcggcac
    20161 gcacggtctg cacgtcactt ccggtcttat ctggatggcg gtgctgatgg tacaaatcgc
    20221 tcgtcgcggc ctgaccagca ctaaccgtac ccgcatcatg tgcctgagcc tgttctggca
    20281 cttcctggat gtggtttgga tctgtgtgtt cactgttgtt tatctgatgg gggcgatgta
    20341 atgagtcatt ctaccgatca cagcggcgcg tcccatggca gcgtaaaaac ctacatgaca
    20401 ggctttatcc tgtcgatcat tctgacggtg attccgttct ggatggtgat gacaggggct
    20461 gcctctccgg ccgtaattct gggaacaatc ctggcaatgg cagtggtaca ggttctggtg
    20521 catctggtgt gcttcctgca catgaatacc aaatcagatg aaggctggaa catgacggcg
    20581 tttgtcttca ccgtgctaat catcgctatc ctggttgtag gctccatctg gattatgtgg
    20641 aacctcaact acaacatgat gatgcactaa gagcggcggt tatgatgttt aagcaatacc
    20701 tgcaagtaac gaaaccaggc atcatctttg gcaacctgat ctcggtgatt gggggattcc
    20761 tgctggcctc aaagggcagc attgattatc ccctgtttat ctacacgctg gttggggtgt
    20821 cactggttgt ggcgtcgggt tgtgtgttta acaactacat cgacagggat atcgacagaa
    20881 agatggaaag gacgaagaat cgggtgctgg ttaaaggcct gatctctcct gctgtctcgc
    20941 tggtgtacgc caccttgctg ggtattgctg gctttatgct gctgtggttt ggcgcgaatc
    21001 cgctggcctg ctggctgggg gtgatgggct ttgtggttta tgtcggcgtc tatagcctgt
    21061 acatgaaacg ccactcagtc tacggcacat tgattggttc gctctccggc gctgcgccgc
    21121 cggtgattgg ctactgtgca gtaaccggcg agttcgatag cggcgcagcg atcctgctgg
    21181 ctatcttcag cctgtggcag atgcctcact cctatgccat cgccattttc cgctttaagg
    21241 attaccaggc ggcaaacatt ccggtattgc cagtggtaaa aggcatttcg gtggcgaaga
    21301 atcacatcac gctgtatatc atcgcctttg ccgttgccac gctgatgctc tctcttggcg
    21361 gttacgctgg atataaatat ctggtggtcg ccgcggcggt tagcgtctgg tggttaggta
    21421 tggctctgcg cggttataaa gttgctgatg acagaatctg ggcgcgcaag ctgttcggct
    21481 tctctatcat cgccatcact gccctctcgg tgatgatgtc cgttgatttt atggtaccgg
    21541 actcgcatac gctgctggct gctgtgtggt aacaaaacct ctctattaaa aaggtgctac
    21601 ggcacctttt ttattgtctg agatcagaga tacggctaat aaccgtatgt cttgacagag
    21661 tgagcagatg aacataaatt aatcatacgg ttgatagccg tattatgtat gagatcgact
    21721 ccctacgatt tcattcagtt aatcaaagag gtcataatgc ctggaaaaac agccactcta
    21781 cccgatgtcg ataaaacgct gaaaaatgcg cgggtcgaac tcaaaactag tcccgacgcc
    21841 aaaaacaaac ttcgtgaagc tgcgcaagcc gttggggtag atttaagcgc ttttattttg
    21901 agcgctgcta tggaacgcgc cgaaagtgtg cttgataacc aacgtcgccg tgagctttcg
    21961 aatcaaagct gggaactgat gaaccaactc atcgctgaac ctgctcaacc gacgctcgcc
    22021 ctcaaggcgt taatgaaaag gaaaaacagc gatggtcgac aagcatgaag agattactct
    22081 gcccatagtc ctctcctgta attatcagtc tgatattact tatcctgggc aaaaacagtt
    22141 tgattgcggt aaccctgtta tcgataaatt tgtacgcgca tcgctaaaga aaagtgtgcg
    22201 taatagcgac tgtgcggcta aagcacttat tgacagacaa agtggtgaac tgatcggcat
    22261 ctgtactttt acggcatatt cgctggaaaa acaacgcgtt tctggcgtcc ttcagggttc
    22321 acaaccttca gaaattggtg ttgtcagatt agtcatgttg ggggtagcac ggaagtatca
    22381 aaagcggggc tttggtcagg acctactatg tgattttttt gaacatgtaa aaataattca
    22441 ccaggcatta ccaattaaag gggtttatct tgatgctgac cctgccgcca ttaattttta
    22501 tgctcgtctc ggctttgttc agctttcagc gacaccaaat gcttttggtg ctgtacctat
    22561 gtttttggcg attcagcata ttctcgcggc ttagattatt atcgccaccc gccagggcaa
    22621 gttttgcctc agccgggggc tgagcagcgt taccactttt gttcaattgc ccccgctttc
    22681 agcaactcca catccttgcc gttaatggtt gccgttgcgg caacgattgt ggtccttaca
    22741 gttgcacaag attttcgctc aaattccggt ttataaagaa taagagacga attactggta
    22801 aatgaaccaa ataggtaatc agtaaagcta atattcagcc ggtttaaacc gaaaggcgcg
    22861 aagccaaccg tttggttaat ctccttaccg gatgggcgac gaatgctgaa tgtcacgcga
    22921 agattggcca cttcgcctaa tttattggtg gtggcgaaga ggctatccat gcaggaaaaa
    22981 cctttatcta aaaaattatc tcgcatccca gaacgggtaa ccgtaatcac gggtaacgta
    23041 tcgtcaaccg tttttctgtc aatcatgtct tttcgacaag tagccagaaa gtcttctggg
    23101 ccaccataaa tgtggacacc tttatcaaga tattccttcg ctttcttaca atctacctca
    23161 acatactgac cattggcgta cataatgccc aacctggacc ttgctaaatt cacaccgtgc
    23221 tgcgcggcct gattaaatag tgcatacgct ttggcataat ccacccgcac ctgctcaccg
    23281 tcgtagtaga taccccccag cgctaacgga gcttctctgt caccttgttt agccactaac
    23341 tcgaaccatt taatcgcttc ctgaacatca ggtttggtat cataacgagg ctgataatat
    23401 tcccagccta acaggctctg cgcgtcgata tctcccgatt cagccatttt atgcagtgta
    23461 tttataacat cgggagcagg tttaccaccg tatatgctgt catacaattc atgcgctttc
    23521 tcgctggcca ccacgcttgg tgaaactatc agcattacag aaaataatat tcttttagtt
    23581 aacatgtata cctttatgtc ctcactgtca tatatttcca taataattaa ccaccaaata
    23641 tccatttaat gaataaatac agtgccataa aggtccaagt aataagcaca catttagcct
    23701 gaaacattat catccatgtc accaaatcgt ttatttagtg agataaaact agcatattcg
    23761 taagataaaa aatgcacata gcaatcaaga aatgatatta ttactttttt gatgtaaaac
    23821 tatttggttt taaaattcat tttttcaaca tatagagcat tcattttatg aatgcaaaaa
    23881 aacatacctg acacacaaat tactctcctc tgtaatattt tctcattaac actctgttta
    23941 cccaacatgc gctccgcact acactatgtg ggcatttttt taaggggtgg taatgaacga
    24001 ttataaaatg acgccaggtg agaggcgcgc gacctggggt ttagggaccg tattctcgtt
    24061 gcgcatgctg ggcatgttta tggttctgcc ggttctgacc acgtacggca tggctctgca
    24121 aggtgccagc gaagcattaa tcggtattgc cattggtatt tatggtctga ctcaggccgt
    24181 ttttcagatt ccgtttggcc tgctttccga ccgcattggt cgcaaaccat taattgtcgg
    24241 tgggctggcg gtgtttgccg ccggtagcgt tatcgctgcg ctttccgact ccatctgggg
    24301 aattattctg ggccgggcgc tacaaggctc tggagcaatt gccgccgccg ttatggcgct
    24361 gctttccgat ctcacgcgcg aacaaaaccg caccaaagcg atggcgttta tcggcgtgag
    24421 ctttggcatt acctttgcca ttgcgatggt gcttggcccg atcatcactc acaaacttgg
    24481 gctgcacgcg ctgttctgga tgatcgctat tctggcaaca accggcattg cgttgaccat
    24541 ttgggttgta cccaacagta gcactcacgt acttaatcgt gagtccggaa tggtgaaagg
    24601 cagtttcagt aaagtgctgg cggaaccgcg gctgctaaaa ctcaactttg gcattatgtg
    24661 tctgcacatg cttctgatgt ctacgtttgt tgccctgccc ggacagctag ctgatgcggg
    24721 gttcccggcg gctgaacact ggaaggtcta tctggcgacg atgttaatcg cctttggctc
    24781 ggtcgtgcct ttcattatct acgctgaagt taagcgcaaa atgaagcaag tctttgtctt
    24841 ctgcgtcggg ttgatcgtgg ttgcggaaat tgtgttgtgg aacgcccaaa cgcagttctg
    24901 gcaactggtg gtcggcgtgc agcttttctt tgtggcgttt aatttgatgg aagcccttct
    24961 gccctcactt atcagtaaag agtcgccagc aggttacaaa ggtacggcga tgggtgtcta
    25021 ctccaccagc cagtttcttg gcgtggcgat tggcggttcg ctgggcggct ggattgacgg
    25081 catgtttgac ggtcaggggg tatttctcgc tggcgcaatg ctggccgcag tgtggctggc
    25141 agtcgccagt accatgaaag aaccgccgta tgtcagcagt ttgcgcattg aaatcccggc
    25201 ggacattgcc gcaaacgaag cgttaaaagt gcgtttgctg gaaactgaag gcgtcaaaga
    25261 agtgttgatt gcagaagaag aacattcagc ttatgtgaaa atcgacagca aagtgacgaa
    25321 tcgctttgag atagaacagg caattcgtca ggcataaaaa acgcggcgaa ttgcctgctg
    25381 cgctacgctt atcaggccta caaagtttct gaattacaaa cctttgtagg ccgaatcagg
    25441 cgttcatgcc gcatctgaca tgaacaaaac gcacatagtc gcgattaatc gcggaagttt
    25501 ttgaactgga acggctgacc gagatcgcca ccacgtacca tcgccatgac agcctgcaaa
    25561 tcatcacgag atttgcccgt tacgcggatt tcatcgccct gaatttgcgc ctgcactttc
    25621 agtttgctgt ctttgatcat cttgacgatt ttcttctggg tcgcgctctc aatgccctgt
    25681 ttcagtttcg cttccacaaa ccaggtttta ccgctatgaa cgatattctc cggtacatcc
    25741 agcgaactgc cttcaatgcc gcgcttcagc agcttggcac gcagaatatc cagcaactga
    25801 ttgacctgga aatcggactc gctcaacact ttgatggttt tgctggcgtc gttcagctca
    25861 aatgaggctt caacgttacg gaagtcaaaa cgggactcca cttcgcggct cgcgttatcg
    25921 accgcgttac gtgcttcctg aagatcaact tcagagacaa tatcgaaaga tggcatcttt
    25981 tcctctccct tcatttttga tgcgaagcat aatacccgca aagttaaata accgacagtc
    26041 ctgcaccagg acgtatccct taattgtgca tagtcgcaac agcatgcaca attaagggat
    26101 agccttattg aacacccgga gtggttgcgg gtgaggagga acaatgaaaa ttaccgtatt
    26161 gggatgcggt gccttagggc aattatggct tacagcactt tgcaaacagg gtcatgaagt
    26221 tcagggctgg ctgcgcgtac cgcaacctta ttgtagcgtg aatctggttg agacagatgg
    26281 ttcgatattt aacgaatcgc tgaccgccaa cgatcccgat tttcttgcca ccagcgatct
    26341 gctcctggtg acgctgaaag catggcaggt ttacgatgcc gtcaaaagcc tcgcgtccac
    26401 actgcctgta accacgccaa tactgttaat tcacaacggc atgggcacca tcgaagagtt
    26461 gcaaaacatt cagcagccat tactgatggg caccaccacc catgccgccc gccgcgacgg
    26521 caatgtcatt attcatgtgg caaacggaat cacgcatatt ggtccggcac ggcaacagga
    26581 tggcgattac agttatctgg cggatatttt gcaaaccgtg ttgcctgacg ttgcctggca
    26641 taacaatatt cgcgccgagc tgtggcgcaa gctggccgtc aactgtgtga ttaatccact
    26701 gaccgccatc tggaattgcc cgaatggtga attacgtcat catccgcaag aaattatgca
    26761 gatatgcgaa gaagtcgcgg cagtgatcga gcgcgaaggg catcatactt cagcagaaga
    26821 tttgcgtgat tacgtgatgc aggtgattga tgccacagcg gaaaatatct cgtcgatgtt
    26881 gcaggatatc cgcgcgctgc gccacaccga aatcgactat atcaatggtt ttctcttacg
    26941 ccgcgcccgc gcgcatggga ttgccgtacc ggaaaacacc cgcctgtttg aaatggtaaa
    27001 aagaaaggag agtgaatatg agcgcatcgg cactggtttg cctcgcccct ggtagtgaag
    27061 agactgaagc cgtcaccact atcgatctgc tggttcgcgg cggtatcaaa gtcaccactg
    27121 ccagcgtcgc cagcgatggc aacctggcca ttacctgctc gcgcggcgtg aagctgctgg
    27181 cggatgcgcc gctggtcgag gtggctgatg gcgaatatga cgtgatcgta ctgcctggtg
    27241 gcattaaagg cgcagagtgt tttcgcgata gtaccctgct ggttgaaacc gttaaacagt
    27301 tccaccgttc cgggcgtatc gtcgcggcta tttgcgccgc gccagccacc gtgctggtgc
    27361 cgcacgatat cttcccgatt ggtaatatga ccggcttccc gacgctgaaa gacaaaattc
    27421 ccgctgaaca atggcaggac aagcgcgtcg tctgggatgc acgggtaaaa ttgctgacca
    27481 gccaggggcc ggggacagct atcgactttg gtctgaaaat tatcgacctg ctggttgggc
    27541 gcgagaaagc ccatgaagtg gcatcacaac tggtgatggc ggcagggatt tataattatt
    27601 acgagtagtg tcggatgcgg taagcgtcgc atctgaccag atgcgacgtt acaaccacaa
    27661 attacgggcg atataccttc acattgttaa agccctgctc gcgcagatag agcgcctgca
    27721 gacggctcat caccccgcgc tcacaccaca gtagccaggt tctgttctgg tcgagatcgc
    27781 caaacttggt gctcagttta tagaacggca gagaaactac atcaatccct tcgactttca
    27841 gtggcttatc ttcctgttca tcgatagaac ggatatcgag gatcacgtcg ttcgggccga
    27901 agccattgac ggtttccact tccaccactt cctgctcggt ctgctgggcg atttcgcgga
    27961 tatcaacgtt attcgcttct tcaaccactt tatcgagaat gctgaagtcg aatttctctt
    28021 cttccgcttc aatcttcgac ttaaccgctt tcaccgtcgg gcttttggag ataacaccgc
    28081 aatattccgg catcgtgcga gcaaagtctt cggtgccaat ctggcgagcc aggttgatga
    28141 tgtgctcttt gtcgtaagaa atcagcggac gcaggatcag cgtatcggag acgttatcaa
    28201 tcaggcgcag gttggtcagc gtctgactgg acacctggcc gagcgcttcg ccggtgacca
    28261 gcgcctgtac gccgtaacgt tcagccactt tagacgcggc acgcaccatc atacgtttga
    28321 ggataacgcc catctgaccg tcgtcgattt tctcgagaat ttccccgacg accggttcga
    28381 aattaatagc gacaaaacgc acgcggtggg agctgccaaa acggttccac agataatgcg
    28441 ccacctgacg cacgccaatt tcatgcgccg cgccgccaag gttaaagaaa cagtaatgca
    28501 cgcggcagcc gcgacgcatc aacatataac tggaaacacc ggagtcgaaa ccaccggaaa
    28561 tgagcgacag cacatcttcc tgggtgccga tcgggaaacc gccaatacct tcgtagcggc
    28621 ctttaatcag caggagacga tcgtcttcca cttccagatg gacagtcact tccggattgg
    28681 tcagcttcac gcgcgcggac tcaatatgct gatttaaacc gccgccgacg taacgttcca
    28741 catcaatcga gctaaaatca tgtttgccac ggcgcttcac gcgtacgcag aaggttttgc
    28801 cttccagctg atcgcgatac tgaaccaacg ctttctcaaa aatatcgtgc atgtcggtaa
    28861 acggcacgtc ttcgacttcg agaatatggt ggatacccgg aatacgggtc agggcgtcgc
    28921 gaatagccag acgctggttt tcatcttttg cgcgaacttc gatgttatcc cagtggcgga
    28981 cgacagcgag cgtctcatca tagtgcttta aaacgttacg aatgttcccg gtaaggattt
    29041 ttataaagcg caagcgcaca gattggcttt tgatggtgat ttccgggaac aatttaatga
    29101 taaacttcat ggcggcaatg gttcgttggc aagccttaag cgacttgtat agggaaaaat
    29161 acagcagccc acacctgcgg ctgcatccag gcgcggaagt ataccactaa catcgctttg
    29221 ctgcgcacat caccttacca ttgcgcgtta tttgctattt gccctgagtc cgttaccatg
    29281 acggggcgaa aaatattgag agtcagacat tcattatgcc gaagaaaaat gaggctcccg
    29341 ccagctttga aaaggcgctg agcgagctgg aacagattgt aacccgtctg gaaagtggcg
    29401 acctgccgct ggaagaggcg ctgaacgagt tcgaacgcgg cgtgcagctg gcacgtcagg
    29461 ggcaggccaa attacaacaa gccgaacagc gcgtacaaat tctgctgtct gacaatgaag
    29521 acgcctctct aacccctttt acaccggaca atgagtaatg gactttccgc agcaactcga
    29581 agcctgcgtt aagcaggcca accaggcgct gagccgtttt atcgccccac tgccctttca
    29641 gaacactccc gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta agcgtctgcg
    29701 acccttcctg gtttatgcca ccggtcatat gtttggcgtt agcacaaaca cgctggacgc
    29761 acccgctgcc gccgtagagt gtatccacgc ttactcatta attcatgatg atttaccggc
    29821 gatggatgat gacgatctgc gtcgcggttt gccgacctgc catgtgaagt ttggcgaagc
    29881 aaacgcgatt ctcgctggcg acgctttaca aacgctggcg ttctcgattc taagcgatgc
    29941 caatatgccg gaagtgtcgg atcgcgacag aatttcgatg atttctgaac tggcgagcgc
    30001 cagcggtatt gccggaatgt gcggtggtca ggcattagat ttagacgcgg aaggcaaaca
    30061 cgtacctctg gacgcgcttg agcgtattca tcgtcataaa accggcgcat tgattcgcgc
    30121 cgccgttcgc cttggtgcat taagcgccgg agataaaggg cgtcgtgctc tgccagtact
    30181 cgacaagtac gcagagagca tcggccttgc cttccaggtt caggatgaca tcctggatgt
    30241 ggtaggagat actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac ttggtaaaag
    30301 tacctaccct gcacttctgg gtcttgagca agcccggaag aaagcccggg atctgatcga
    30361 cgatgcccgt cagtcgctga aacaactggc tgaacaatca ctcgatacct cggcactgga
    30421 agcgctagcg gactacatca tccagcgtaa taaataaaca ataagtatta ataggcccct
    30481 gatgagtttt gatattgcca aatacccgac cctggcactg gtcgactcca cccaggagtt
    30541 acgactgttg ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc gctatttact
    30601 cgacagcgtg agccgttcca gcgggcactt cgcctccggg ctgggcacgg tcgaactgac
    30661 cgtggcgctg cactatgtct acaacacccc gtttgaccaa ttgatttggg atgtggggca
    30721 tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca ccatccgtca
    30781 gaaaggcggc ctgcacccgt tcccgtggcg cggcgaaagc gaatatgacg tattaagcgt
    30841 cgggcattca tcaacctcca tcagtgccgg aattggtatt gcggttgctg ccgagaaaga
    30901 aggcaaaaat cgccgtaccg tctgtgtaat cggcgatggc gcgattaccg caggcatggc
    30961 gtttgaagcg atgaatcacg cgggcgatat ccgtcctgat atgctggtgg tcctcaacga
    31021 caacgaaatg tcgatttccg aaaatgtcgg cgcgctcaac aaccatctgg cgcagctgct
    31081 ttccggtaag ctttactctt cactgcgcga aggcgggaaa aaagttttct ctggcgttcc
    31141 gccaattaaa gagctgctca aacgtaccga agaacatatt aaaggcatgg tagtgcctgg
    31201 cacgttgttt gaagagctgg gctttaacta catcggcccg gttgacggtc acgatgtgct
    31261 ggggcttatc accacgctga agaacatgcg cgacctgaaa ggcccgcagt tcctgcatat
    31321 catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa gaccccatca ctttccacgc
    31381 cgtgcctaaa tttgatccct ccagcggttg tttgccgaaa agtagcggcg gtttgccgag
    31441 ctattcaaaa atctttggcg actggttgtg cgaaacggca gcgaaagaca acaagctgat
    31501 ggcgattact ccggcgatgc gtgaaggttc cggcatggtc gagttttcac gtaaattccc
    31561 ggatcgttac ttcgacgtgg caatcgccga gcaacacgcg gtgacctttg ccgccggtct
    31621 ggcgattggt gggtacaaac ccattgtcgc gatttactcc actttcctgc aacgcgccta
    31681 tgatcaggtg ctgcatgacg tggcgattca aaagcttccg gtcctgttcg ccatcgaccg
    31741 cgcgggcatt gttggtgctg acggtcaaac ccatcagggc gcttttgacc tctcttacct
    31801 gcgctgcata ccggaaatgg tcattatgac cccgagcgat gaaaacgaat gtcgccagat
    31861 gctctatacc ggctatcact ataacgacgg cccgtccgcg gtgcgctacc cgcgcggtaa
    31921 cgcggttggc gtggaactga cgccgctgga aaaactgcca attggcaaag gcattgtgaa
    31981 gcgtcgtggc gagaaactgg cgatccttaa ctttggtacg ctgatgccag acgcggcgaa
    32041 agtcgctgaa tcgctgaacg ctacgctggt cgatatgcgt tttgtgaaac cgcttgatga
    32101 agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag aagaaaacgc
    32161 cattatgggc ggcgcaggca gcggcgtgaa cgaagtgcta atggcccatc gtaaaccagt
    32221 acccgtgctg aacattggcc tgcctgactt ctttattcca caaggaactc aggaagaaat
    32281 gcgcgccgaa ctcggcctcg atgccgccgg tatggaagcc aaaatcaagg cctggctggc
    32341 ataatcccta ctccactcct gctatgctta agaaattatt catagactct aaataattcg
    32401 agttgcagga aggcggcaaa cgagtgaagc cccaggagct tacataagta agtgactggg
    32461 gtgaacgaat gcagccgcag cacatgcaac ttgaagtatg acgagtatag cgggagtggc
    32521 agcatgcaat acaacccctt aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc
    32581 tgtatgacct ttggcgagcc agatcgcggt aatcacgcat ggacactgcc ggaagagagc
    32641 agccgtccca tcattaaacg cgcgctggaa ggcggcataa atttctttga taccgccaat
    32701 agctattccg atggcagcag cgaagagatc gttggtcgcg cactgcggga tttcgcccgt
    32761 cgtgaagacg tggtcgttgc gacgaaagtg ttccatcgcg ttggtgattt accggaagga
    32821 ttatcccgtg cacaaatttt gcgctctatc gacgacagcc tgcgccgtct cggcatggat
    32881 tatgtcgata tcctgcaaat tcatcgctgg gattacaaca cgccgatcga agagacgctg
    32941 gaagccctga acgacgtggt aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg
    33001 cacgcttcgc agtttgctca ggccctgaag ctacaaaaac agcacggctg ggcgcagttt
    33061 gtcagtatgc aggatcacta caatctgatt tatcgcgaag aagagagcga gatgctgcca
    33121 ctgtgttatc aggagggcgt ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg
    33181 acgcgtccgt ggggagaaac taccgcacga ctggtgtctg atgaggtggg gaaaaatctc
    33241 tataaagaaa gcgatgaaaa tgacgcgcag atcgcagagc ggttaacagg cgtcagcgaa
    33301 gaactcggtg caacacgagc acaagttgcg ctggcctggt tgttgagtaa accgggcatt
    33361 gccgcaccga ttatcggaac ttcgcgcgaa gaacagcttg atgagctatt gaacgcggtg
    33421 gatatcactt tgaagccgga acagattgcc gaactggaaa cgccgtataa accgcatgcg
    33481 gtcgtaggat ttaaataaga gtccaggcct gatgagacgt gacaagcgtc acatcaggca
    33541 tcggtgcaca actacgacag aatacccagc ggccagtgat gaccgataaa gtacaggatg
    33601 cctgcggaaa tcaccccggc gacaatatcg tcgatcatga tccccatgcc gccatgtaca
    33661 ttgcgatcaa accagcggat cggccacggc ttccacatat ccagaatacg gaaaatcaca
    33721 aacccggcgg caacccactg ccagtcattg gtcggcagcg ccatgagcgt gatccacata
    33781 ccaataaatt cgtcccagac aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt
    33841 tgatgacaaa gatagacgcc gatacagatc cccaacatca ccaccagcga gtagagctgc
    33901 cagggtaaaa aggtcatcag ataccagaac ggaatcgctg ccagcgatcc catcgtgcca
    33961 ggaacgattg ggcttaatcc acttccgaat ccgacagcaa gcagatgcca cggattactc
    34021 atcttcaggc gacttttcgc gacatcttta tggcgtggca aaatggtcat atcctttcca
    34081 gtcaaacgtg acaggttcgc cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat
    34141 ttgcccgata caggtaaacg gtacgcccag gtgtccgaga gccacatcca gcgcgccacg
    34201 gttcagttcc ggcacagtga aacacaactc gtaatcttca ccgccagaga gcgcccagcg
    34261 cagcgcctgt tccggttcaa catggcgaga aagcgcatca gaaaacggca gcaatgccag
    34321 gtcaatacgt gcgccgcagt cgctggcttt cacgatatgc ccgagatcgg aaatcagacc
    34381 gtcagagaga tcgatagctg aatttgccag atcgcgcagt gcctgcccct gtaaaatacg
    34441 cggcgatgga cggagatgac gtttgatcaa gtagtcagca tctttagcat cggcaacctg
    34501 caaacggttt tgcaaaatcg ccagcccggc ggcgctatcg cccggtgtac cggtcacata
    34561 gatccagtca cccggtttcg ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc
    34621 gtggataccc aacgtcattg ataatggccc acgcgtggta tcgccgccaa tgagttgcat
    34681 atcgtaataa ttgagaagat caaacaaact gtcgctgaag gactcaagcc acgcttcgtc
    34741 tacgtccggt aaggttaatg ccagcgtcag ccaggccgga tcggccccca tcgctgccag
    34801 atcgcttagg ttcactgcca gtgctttata cgccagatca gcaggatcga tatcatggag
    34861 gaagtggttg cccgccacca gcgtatcagt gctgatcgcc agggtctgtt tctcggggat
    34921 attgagaaga gcgcaatcgt cgccgatgcc cagttcgaca tcaagacgag aacttcttac
    34981 acggtcaaaa taacgggcaa tcagggagaa ctcgccacat gccatacgtt atgcctcagc
    35041 aggtaaaaaa gaaaaggccg gcgatcgcgg aacggtcttc cgtgaatcta ccggcctgga
    35101 tatcactttt tgttagggcg aatcacaggt gctgctttat cgagtacgcc gttgacgaac
    35161 ttatggctgt cttctgcgcc gaacgatttc gccagttcga tcgcttcgtt aatggccact
    35221 ttgtatggca catcgctacg tttagacagc tcgtacagcg caatgcgcag tactgctttt
    35281 tctacctgtc ccagttcttc cagcaggcgg gacaggtatg gcttcatcag tccgtcgagg
    35341 tatgcggtgt tagtcgccac cccggccagc agctcacgga agtacaggac gtcaacgtct
    35401 tttacatcct gttcagccag gaactggtat tcaacatcag cgatgtcgtt ctgggacaac
    35461 tgccaggagt agagcgcctg gacggcacac tcacgagcgc ggcgacgagc agcaggtttc
    35521 acggatttcc ccttactaat ttcaggcctt gatggctttc aatacattaa tcatttcaag
    35581 cgcggtcagt gcagcttctg cacctttgtt gccagctttg gtgccagcac gttcgatcgc
    35641 ttgttcaatg ctttcagtgg tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc
    35701 aacatgcgcc aggccgttgc ttgcaccacc agcgacatat tcaaagtggg cagtgccacc
    35761 acgaataacc gtacccagcg caatcaccgc gtcgtattta ccggttttag ccagtgcacc
    35821 cgccgccagc ggcagctcat aggcaccagg cacccaaaca acggtaatgt tttcatcttt
    35881 tacctgaccg atacgtttca gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa
    35941 gttgttgaaa cgcgcgatgg tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc
    36001 aataatgttc attttcttcc ttcgggttcg agtatggccc cgcagggggg cggattttag
    36061 cataatattt cgtgcgctgc ttccctttcg agccgggaga tcatgcaccc actaaatgca
    36121 ggcaaacatc cgggcctaca tgacgtatct ctttgaattt aaattggggg gcgtcggcta
    36181 atttctcaag ccctggcagc gtgcataatc cgcgggcgtc gctgcctaat agtttaggtg
    36241 cgatatagac aatcagctca tcgactaaac ccgcctgcag caatgcgcca gcgagcgttg
    36301 gccccgcttc cacccagata ctgttaattt gctgtttacc cagttgcatc atcagtacaa
    36361 ccagatccag atgaccttta tgctctggaa tcagcaaggt acgcaccgtt tccggccact
    36421 cactagaatc ttcctgcgta cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat
    36481 gttccggcgt cacgcgattt tggctatcaa tcacaatacg tactggctgg cggagatttt
    36541 gttgcgggta gagcgcctga gtttgttcat ccagttcaga ccaacgcact gttaaggctg
    36601 gatcatccgc cagcaccgtg gcgctgctag ttaaaatggc atgactttgt gcgcgctgtc
    36661 gttgtacgtc gcgccgcgcc tgaggcgaag tgatccactg gctttcgccg ctcgccatcg
    36721 ccgtgcggcc atcaagcgat gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca
    36781 tccgcttgag aaagccttta ttcaattgct cggcttcact catcatcagc ccgtggctga
    36841 cgtcaatgcc agcctgttgc agacggtaaa gtccacgccc agcgacctgc gggttaggat
    36901 cttgcatcga ggcaaccacg cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg
    36961 gcgtacgacc atgatggcta cagggttcga gtgtgacata ggcggtcgca cctttggctt
    37021 tttcacccgc catacgcaac gcgtgtactt cggcatgtgg ttcacccgca cgttggtggt
    37081 aaccttcacc gacaatttcg ccatctttga caatgacgca cccgacattc gggttgggat
    37141 gcgtggtaaa acgtcctcgt tgcgccagct ttagcgctcg cgccatgtaa tactcgtcct
    37201 gcacggctta gtcctccagg cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac
    37261 tgcggtagac agaggcaaaa cggatatagg cgactttatc gagctttttc aattgctcca
    37321 tcaccagatt gccaatcatc ttgctcggca cttcgcgctc accggtggcg cgcagctgcg
    37381 atttaatatg attgattgcc atttcgacgt catcggaact caccggacgt ttttccagcg
    37441 cccgcagcat tccgctacgc aatttctctt cattaaacgg ttcacgcacg tcgttgcttt
    37501 ttacaacacg cggcataacc agctccgcca cttcaaaggt ggtgaaacgt tcattacaca
    37561 ccagacactg ccgacggcgg cgtacggatg aaccctcgcc cacgagacga gagtcaatta
    37621 ccttagtgtc cacggcgaaa cagaatgggc aatgcatacg gcgtcctgac caggtggtta
    37681 acagaaatct attttaccct gaactgacga tacaacaaag gcgcaacgct tttgagataa
    37741 aggatcgatg ctttcgctgc ttttaccctc taccattagg ctaattgcga cgagaaaggg
    37801 acgacctatg aatactaacg tttttcgact gctcctgctg ggaagcctgt tcagccttag
    37861 cgcctgtgtg cagcagagcg aagtgcgaca gatgaaacac agcgtcagca cgctgaacca
    37921 agagatgacg caactcaata aagaaacggt caaaatcact cagcaaaaca ggctgaatgc
    37981 aaaatccagc agtggggttt accttctgcc aggagcgaaa acaccggcaa gactggaaag
    38041 ccagatcggt actttacgta tgtcgctggt gaatattacg cctgatgcag atggcaccac
    38101 cctgacgcta cgtattcagg gcgagtcgaa tgatcccttg cccgcattca gcggaactgt
    38161 tgaatatggg caaattcagg gaacaataga caactttcag gaaatcaatg tgcagaatca
    38221 attgattaat gcccctgcca gcgtcctcgc ccccagcgat gttgatattc cgttacagtt
    38281 aaagggtatt tctgtagaac agttagactt cgtacgtatc cacgatattc aacctgttat
    38341 gcagtaaacg tatttcgggg cgattttgtg cgtctcgcaa catctttccc cgtcattttg
    38401 ttactccgct tacatcaccc tgattgatag taaaagtttg caacaagggt gaaagtcagt
    38461 acaatccctg cccgaatgtg tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact
    38521 gtgaaacgaa acatattttt gtgagcaatg atttttataa taggctcctc tgtatacgaa
    38581 atatttagaa acgcaatttg cgcctttttc actcccgcaa gggattttca aacagtggca
    38641 tacatatgaa aaaaacatta ctggcagccg gtgcggtact ggcgctctct tcgtctttta
    38701 ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc cgactggtgg caccagagcg
    38761 ttaacgttgt cggaagctat cacacccgtt tcggaccgca gatccgcaac gatacctacc
    38821 ttgagtacga agcattcgct aaaaaagact ggttcgactt ctatggttat gcggatgcgc
    38881 cggtattctt cggcggtaac tccgatgcta aaggtatctg gaaccacggt tctccgctgt
    38941 ttatggaaat cgaaccacgt ttctccatcg acaagctgac caatactgac cttagcttcg
    39001 gtccgttcaa agagtggtac ttcgcgaaca actacattta cgacatgggt cgtaataaag
    39061 atggtcgcca gagcacctgg tacatgggtc tgggtaccga catcgatact ggtctgccga
    39121 tgagcctgtc catgaacgtc tatgcgaaat accagtggca gaactatggc gcagcgaacg
    39181 aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt
    39241 ggggcggtca gctgagctac atcggcttca ccaacttcga ctggggttcc gatttagggg
    39301 atgacagcgg taacgcaatc aacggtatta agacccgtac taataactct atcgcttcca
    39361 gccatattct ggctctgaac tacgatcact ggcactactc tgtcgtagct cgttactggc
    39421 acgacggtgg tcagtggaac gacgatgcag aactgaactt cggcaacggc aacttcaacg
    39481 ttcgctctac cggctggggt ggttacctgg ttgtaggtta caacttctga ttatgaaaat
    39541 gccgggattt attcccggca tttcagattg ttaacaacgt gcgctttgtt catgccggat
    39601 gcggcgtaaa cgctttgtcc gacccacaaa agcacgtaaa ttcaatatat tgctgaaata
    39661 acgtagtcct gataagcgta gcgcatcagg caattttacg ttttaatcac ttcttcttgt
    39721 tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg
    39781 catcttcccc tgcgataacg gtcgtaccat actgatccgc ttcggtatat ttcgaatgct
    39841 catgatcgtg gcaatagaga caaagcaatt cccagttact gccatcttcc gggttattgg
    39901 tatggtcgtg atcaatgtgg tgaacagtaa gttcacgcag gttggaataa acaaactcgc
    39961 gggaacagcg accgcagacc cacggatata tttttaatgc tttttcgcga tagccgcttt
    40021 ctaaccgcgc atagtttttt gggatgatag ccatgaagag ttacctggat gaaatgtagg
    40081 gttacgcgca gtttcccaag atgcggggat tttcgcaatc tccatacatc aaaaaaaatc
    40141 ccgatcttct gaccgggatt ttcaacatca acgggaactt gattacggca gaattgacgg
    40201 ctgatccgcc ccttcttttt ccactttctg ctgcaacatg tgttcgcgct tcatacccag
    40261 tttcagagcc aacgcagatg ccacatagat ggaagatgca gtaccgatgg aaacaccgat
    40321 aagcatggtc agcgagaagc cttccagtac cggaccaccg aagaggtaca gcatcaggat
    40381 aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac
    40441 gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg aagttttcac gaatacggtc
    40501 cgataccacg atactgtcgt taagcgagta accgataacc gacatcaacg atgccacaat
    40561 ggtcaggtca atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg
    40621 cgccagcgca ataaccaccc ctgccgccag tcgccactca aagcgaaaac ctacgtacac
    40681 aaggatagac agcagcgccg ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc
    40741 cacgctcgga ccgacgaact caatacgctt cactgctgca ttctgattgg tggattcgtt
    40801 aatcaccttc agaacctggc tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg
    40861 catacggacc atgatgtcgt ggctgctacc aaagttttgc agcattggct cttcaaaacc
    40921 ggctttttgc aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc
    40981 gataaccgta ccaccggtga aatccagccc ccagttaaag ccgcgcacgc ccataataac
    41041 gatagcggcg attaacagca gaccagagat gccgaaagcc cagtagtccc agcgcataaa
    41101 gtcatagact ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca
    41161 ctcctcagat tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac
    41221 gcgtaccgac aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga
    41281 accctttaat tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga
    41341 tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa
    41401 cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga
    41461 ccgccaccgc aagggttaag acgatacccg cgatacctgg catactcagc gttgcgcctg
    41521 gcaacagcga cataatgccg acgattaaga tcaagttggc aatcagagca ctggtcgcaa
    41581 tcagaccaaa cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa
    41641 ggcaagcctc cagcccctgt tcaatgttct gcatacccag ggttgggcca atggtacgtt
    41701 cttcaacaat ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gacagctgac
    41761 gggcttcgtt cgggttgttg atgccggtga tacggaagct gttacccaga cgagactgga
    41821 tgttggcgat gttaatcacc tcttcctgtt tcaccagaac cgcacgacca tttgcatctt
    41881 tcttaccgct gtctttgtac tccacaaaca gggttgccat cggtttgccg atgttgtcct
    41941 tagtgaagtt agacatgatg ttaccaccag cgctatcgag cgagatgtta acctgcggtt
    42001 ggttgtattc gtcctggctg gaagtggagt cggtgatatg gtcaccggtc agaattacgc
    42061 gtttgtacag cacaactggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta
    42121 cgcgaccgga tgccgcagcg gcctggtcaa cgttggtgtt taccagacgg aattccagcg
    42181 ttgccgtcgc acccagaatc tctttcgcac gcgcagtgtc ctgaatacct ggcagttcaa
    42241 caacgatacg gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca agttggttta
    42301 cacggttacg caggatattg atgttctgct gcaccgcata ttcacgcgct tcactcagac
    42361 gagcatcgct cattaccgca cgcagctggt tgctgccctg gctactaatc accaggtccg
    42421 gatggcgctt gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtaa
    42481 tgctcaggcc gtagttgttt tctttacgaa cggtggtata cgggatgccc ttttcacgca
    42541 gatcgctgcg caggctatcg atattttgtt cctggagttt gccaagcgcg gtgtccatat
    42601 ccacttccat caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag
    42661 cgtgaatagc tgccagccag cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt
    42721 tgtcacccat aacgcccatt aatgcttcac gagcgcgcag ctgggtgtca gtggagtcga
    42781 agcgcgcaag aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt
    42841 gtaacgtttt ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag
    42901 tgatctgaac agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga
    42961 caatcaccac gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc
    43021 ccttagggaa aaattttaat tacagcgcct tcatggtgcc tttcggcagg acggcagcta
    43081 cgaagtcacg tttaataact acttcagtgg tgtcattcag cgcgatagca atgtagccgt
    43141 tttccgctac tttggttacg cgaccaacca ggccaccgtt cgtcagaact tcatcacctt
    43201 tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga
    43261 tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgggctac
    43321 cttgcgccgg tgcacccgtt gccgctaccg catcagaaat aaaaaagctc attaaatttc
    43381 cctcattatt aatattaatc aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa
    43441 aaatcagtta cgaagctctc taatttaccc tcttcaatag ccttgcgtaa acccgccatc
    43501 aaacgctggt agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg
    43561 caacggtcaa gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta gcaatcacac
    43621 tcaggatcga gtgggccagt atcgctctta tacttcgcat tgcggatttt caccacgcca
    43681 tcggtcacga acaaatgacc atttcgggcg ttgcgggttg gcattacgca gtcaaacata
    43741 tcgataccac gacgtacgcc ttcaaccagg tcttctggtt taccaacgcc catcaggtaa
    43801 cgcggtttgt ctgccggaat ttgcgggcat acatgctcca gaatgcggtg catatctgct
    43861 ttcggctcac ccacagccag accgccgaca gcgtagccat caaaaccgat atctaccaga
    43921 cctttaacag aaatatcacg taaatcttcg taaacgctgc cctgaatgat accaaacagc
    43981 gcatttttgt ttccgagact gtcaaaacgc tcacggctac gcttcgccca acgcagagac
    44041 atctccatgg agcgttttgc gtaatcccag tcagcaggat acggcgtaca ctcatcaaag
    44101 atcatgacga tatccgaacc aagatcgtac tgaatctcca ttgatttttc aggatcgagg
    44161 aaaatcggat cgccgttgat cgggttacgg aagtgcacgc cctgttcggt gattttacga
    44221 atatcgccaa ggctgaagac ctggaagccg ccggagtcgg tgaggatcgg ccccttccac
    44281 tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg gcgcagccac
    44341 aggtggaagg tgttgccgag gataatttgc gcgccagtgg cttcaacttc ttccggcgtc
    44401 atccctttta cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc cactacgcca
    44461 caatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc
    44521 attttttctc cactacgtca gaaaaacagt ccaacgtttt aaaccagcgc cgcggaatta
    44581 ctccccgacg cgctcattaa ttgcctgcgg attgtacgtg ataaacatcg catcaccgta
    44641 actaaaaaag cgatatttct cttctaccgc tgctttatag gcgttcatgg tgtgttgata
    44701 accggcaaag gccgaaacca gcataatcag cgtcgattcc ggcaggtgga agtttgtcac
    44761 cagcgcatcg accactttgt actggaagcc cggatagata aagatttggg tatcgtcaaa
    44821 gaacggttcg ataagatcgt ttttcgccgc ctgagccgcg ctttccagcg aacgtactga
    44881 ggtggtgcca accgcaatca cccggttacc gcgcgctttc gccgccagta ccgcgtctac
    44941 cacatcctgc ggcacttcag cgtattccga gtgcatgatg tgatcttcaa tggtgtcgac
    45001 gcgcaccggc tggaaggtgc ccgcaccaac gtgcaacgtc acaaacgcca tctccacgcc
    45061 tttggcgcgt aatttttcca gcaaaggttc gtcaaaatgc agacctgcgg tcggggctgc
    45121 aaccgcgccc ggtttttcgc tataaacggt ttgataaagt tcgcggtcag cgtcttcgtc
    45181 cggacggtcg atatacggcg gcagcggcat atggccgatg ctgttgagaa tatccagcac
    45241 cgagcgttca tcattaaatt cgacttcaaa cagtgcgccg tggcgcgcgg tcattgttgc
    45301 gttaatactt tcgtcatcgc ccagcagcag ttctgcgcca ggttttggcg ctttcgaggc
    45361 gcgaatatgc gcaagaatgc gtttgtcgtc gagcatccgt tcaaccagca cttcaatctt
    45421 gccgccgctg gctttacgcc caaacaggcg cgccgggatc acgcgggtat tattaaaaac
    45481 cagaagatcg ccggggttga gcttatcaag taaatcggtg aaagtaccgt gcgtcagcgc
    45541 gcccgtcggc ccgtccagcg acagtaaacg acagctactg cgttcaggca tgggatagtg
    45601 ggcaatcagg gattcgggca attcaaagga gaaatcggta acgcgcatga cactgactca
    45661 gactaaaata agaggcgggt agtctagtgc cggggcgctc tccctgcaac ctttaccccc
    45721 ttttggatag aatataatcg atgaattttt tagctcacct gcatttagcc catctcgcgg
    45781 aaagctcgct ttccggcaat ttactggctg atttcgtacg cggaaatcct gaagaaagtt
    45841 ttccgcccga cgtcgtggct ggcattcata tgcatcgacg tatcgacgta ttaaccgaca
    45901 atctgccgga agtccgcgaa gcacgggaat ggtttcgtag tgaaactcgc cgcgttgcgc
    45961 ctattacgct ggatgtcatg tgggatcact ttctttcccg ccactggtcg cagctgtcgc
    46021 cggactttcc gctacaggaa tttgtctgtt atgcccgcga gcaagtgatg acgattttgc
    46081 cggactcacc gccacgtttt atcaatctga acaattactt gtggtcagag cagtggctgg
    46141 tgcgctatcg cgatatggat ttcatccaga acgtgttaaa cggcatggca agccgccgcc
    46201 cacgtctgga tgccctgcgt gactcctggt acgatttaga cgctcattat gacgccctcg
    46261 aaacccgctt ctggcagttt tatccgcgga tgatggcgca ggcgtcacac aaggcgttat
    46321 aaaacgttag ttcatccata ccgtagccga aatggcaggc aaagccagaa tcccgtcagt
    46381 cagttgccca tgcccttctt tgcattgcca ttgcacggca ttgagcaacg gtgacgcggg
    46441 tagcaccact tcacaagctt cgccacggtt gattgccacc agtacacgct gctgattcag
    46501 cacgcggaca aataccacca cgttatcttc cgcatacagc acctgacagc cgccatgacg
    46561 tagcgcctga cttttcttac gcagcgcaat cattcgctgg tacagcgcga ataacgccgt
    46621 atcctgcttt tccacctgcc aggggaacgg tttacggcaa aacggatcgt ttttgccatc
    46681 cagtcctact tcatcaccgt aataaatgca cggtacacca ggccaggtga acagccagac
    46741 caccgccagc ggcaggcgcg caatatcccg accgagcagc gttttaaatc gcgcagtatc
    46801 gtggctgtcg agctgattaa acatacgtaa ttgttgttga tgagaaagcc ctgcgcggta
    46861 attatccatc caggccatac aggtttgggc atcaatttgc tgcggatcgt aagagatatc
    46921 ggtattggca agaaatcccc acaacgggaa tgtgaagcca cgatagttca tggcggcatc
    46981 ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa tgttcgccga caatatacgc
    47041 ttccggctgg gtttctttcg ccgcttcggt gatcccagca acgtgctgca tattattgcg
    47101 cgccccaccc gcctccccca gcatatgcac cacatccagc cgccagccgt ccatactcca
    47161 cggcgctttc agccaatggc ggacaatact gtcttccccg cgataaattt cattcaccag
    47221 actttccgac tgataatcca gcttcggcaa gctggcatag ccaagccagt cgagcgccgt
    47281 gccatcatca ctaaacgagt accagtcgcg ccagggcgat tcagggttgt gacaagctcc
    47341 ccccgtgcca cgattgtgcc tgtcaaacca ggcatgggaa tcgccactgt ggttaaacac
    47401 gccatccagc accagccgca ttcccagctg ctgcgtattg tggcgtaagc gcagcaacgc
    47461 cccatcaccg tcaaactgcg gatcgacatg gcgataatcc tcggtatcgt atttatgtac
    47521 gctgggagct ttaaacaccg gattgagata cagcgccgtc acgccaagct ttttcagata
    47581 cggcagtttt tcgcttatcc cgtccagatc gccgccatag aacgttgatc cgcccgcctg
    47641 cgccgtgacc ggttcatccc agtcacgcaa gatgatctct tgtccggctg catgatggta
    47701 atagacatga tcctgttcag cttcacgagg aagactacgc gcaaaacgat cagggaagat
    47761 ctgataaaaa atctgatccg cagcccattg tgggccgata tccggtacat cgacggcaaa
    47821 ctgctccagt cgtgccggcg gcattcggct gaagccctgc ggtgtaaacc aacgctggcg
    47881 atcgtgccac agcagtttga aactgtaacg ccgccggggt tgtccgctgg agagatcaat
    47941 cgccgcacgc catgcggtga cgccaggctg ctgctgactg cgctgcttat gcatcgatac
    48001 tgacatttct tcgttatcgt gttctgtacg cagcataatg cgctgcggtg ggtcttcgcc
    48061 cgtcagccac agtgtaatga gcagttgatc tttgctttgt ttaacaaatg ggggcaccgg
    48121 caggtgccat gcatttaaca tcataattcc cctgtgatga aaatctgatc accttgccat
    48181 agcctaatgc aggatcactc atcatctgaa ggatatttgg gggaggagga cgggggcgga
    48241 taactttcgg aatagccgga tcaggatttc ctcctgaacc ggcttacaga acgcaagtat
    48301 tattggtttt cagccagctg acgatcgtgg cggcgtttaa acatccagcc aatcaacagc
    48361 acaacaatcc acgcgaaacc gacatacagc gagatacgcg tatccgggtg ataaccaatc
    48421 aacccgataa taaagagcag gaaaatcagc ccgccgatgg tcgttgctac cccacccggc
    48481 actttaaatt tcagcgcctt aacttcttct ggcggcaaac ggcgacggaa ggcaatttgc
    48541 gacagcagga tcataatcca cacccacacc gtggcgaagg ttgccagcga agcgatcacc
    48601 aggaagacgt tttccggcat gatgtagttc agatacaccg caaacagcag cgcggtagtc
    48661 atcaccagca ccgtaaccca cggaataccg cgacgcgacg ttttgctgaa aattttcggc
    48721 gcgctgccct gctctgccat accgtggagc atacggccta cgccaaatac gtcactgtta
    48781 attgccgaca gcgaagcagt cagcacaaca aagttaagaa tgctggcggc aaaggtaatg
    48841 cccatatgct ggaacgtcag cacgaacggg ctaccggcag tgccaacctg attccacggg
    48901 tagatagaca taatgacgaa cagcgtaccg acgtagaaca ccagaatacg catcggcacg
    48961 gagttaatcg cacgcggtat cgatttctca ggatctttcg cttcaccggc ggtaatcccg
    49021 ataatttcga tcccaccgta agcaaacatc accatttgca acgacatcac catcccaagc
    49081 cagccgttac tgaagaagcc gccgttgctc cacaggttat gaataccggt cggttgcccg
    49141 ccgttgccaa tcccccagat gatgatgccg aaaccggcga caatcatgat gatgatggtg
    49201 gcgactttaa agaacgagaa ccagaattcc agctcaccga agaccttcac gctcatcagg
    49261 tttacggcgc aaatgatcag caccacgctc agtacccaaa tccagtgcgg caccgtcggg
    49321 aaccagacac ccatatagat accaaaagcg gtcacatcgg cgatggcgac aataaggatt
    49381 tcaaagcaat aggtccagcc ggtaatgtaa cctgccagcg ggccgaggtt ttcctgcgca
    49441 taacgcgaga aagagctggc tgccgggtta tgtaccgaca tttcccccag cgcacgcata
    49501 atgatatacg ccgcgatacc accgataata taggccaaca acacgctcgg accggccatt
    49561 ttgatggcgt ctgccgaacc gtaaaacagc ccggtgccaa ttgctgaacc cagtgccata
    49621 aagcgtatgt ggcgggtact tagcccacgc tttagcttgt tcttactttc catcaattcc
    49681 taacccatca acacaataaa aaaccacggg gcatcgcagc cccgtggtta aaacaaatgt
    49741 tcagtgattt agtgagcgct ggaggtcacc tgacgacctg ccgcgcgatc ccagataatg
    49801 gccagaacca ccatcaccac tgttggcatt aaccacgcca gaccttgttc ggccagcggt
    49861 aaacgctgcg cccaggacgg taagatatcg ctgaatgcag atgctttgat cccgtcgaga
    49921 ataccaaaaa gcaggctgat aaacatcggc ggagcaatca cgcgggacga attatgccac
    49981 catgagcgtg taaaacttaa tacaaccagt gcgatacacg gcggataaat agcggtcagc
    50041 accggtacgg agatctggat cagctggctt aagccgaggt tagaaaccac catcgagaag
    50101 ccgccgagga taaacaccag cgtacgataa gagagcggta cgtactgggc aaagaattct
    50161 gcacaagcac aggtcaggcc aactgccgtt accaggcagg cgatgaagat taacgccgcc
    50221 agcaggaagc taccgccgcc gccaaaggtg tgctgaacgt aagcatgcag aatagcagcg
    50281 ccgtttgcag actgatcgac cagcgacgcg ctgtctgaac ccagacggaa cagcgccagg
    50341 tacagcagag tcagaccaac acccgccatc aggccagccc agacggtata acgggtcagc
    50401 agacgcgctt cggtaacgcc acgagaacgc gccgcgttaa caataacgat accaaacacc
    50461 attgcgccca gcgtatccat ggtcagatag ccgttaacga agccgttaga aaacgcagcg
    50521 ttttgataag cctcagtcgc cgtgctgata gaacccgccg gccagacaat cgcggcaaca
    50581 gacaggatga ccagcgcgat aattttcagc ggcgcaagga agttgcccac ggtatccagc
    50641 agcttgcccg gatagagcga aaccagaata acgatagcga aatagaccag gctgtagata
    50701 aacagcggca gcgcggaatc acccgtcagc ggcgcaatcc ccacttcaaa ggaaacggta
    50761 gctgtacgcg gcgtagcgaa aagcggcccc accgccaggt aacaaaccgt tgccagcagt
    50821 acgccagcga ctttaccgat tggcgtgctg aggctgtcaa caccgccgcc aacttttgcc
    50881 agcgccacta ccgttaatac cggcaggcca acggcagtaa tgaggaagcc gaatgccgca
    50941 gtccagacgt gttcgcctgc ctgtaagccg accattggag ggaaaataat gttacctgcg
    51001 ccgacgaaca acgcaaatgt cataaagccc agagcgatga tatcgcgcga tcttaattga
    51061 tgggtcataa atcatactgc ctgtggatgt ggtgttgtaa cgttaaattt ttgtgccaat
    51121 ccctggggat gatacagcgg tatatttaaa caattttagc gggaaatact cccaatcagg
    51181 cgcggcgaag aatagttttt cgatttaccg cgttaatgca gtcagtatga cagcacctga
    51241 agcgcaattt aaacgcttat aacgttttaa agcaaggagg gatctaaaaa ccagaggtat
    51301 atagcgataa gaaatcacca accagtataa aacactattt ccgtgaaaaa gacatggcta
    51361 atcatgcgaa caaaaaatca ccaaccgttg taaaatctaa cgcccgtatt ttgactgcaa
    51421 agaaaataag ccagcatcgg ctggcttatg gcaataaaag atgacaaagg cggattaatc
    51481 gctgtttttg gcaattaaac gttccgggat aacaaaactg aaacgtgttc cttttcctac
    51541 tgtactctca atattcaggc gactttcgtg atgattcacc gcatgtttca cgatcgctaa
    51601 ccctaatccg ctaccgccgg tttgccggga acgcgcttta tcaacgcgat aaaaacgctc
    51661 ggtcaggcgc ggaatatgct ccggtgcaat gcccggtccg ttatcttcaa cgctaaattc
    51721 ggcaccgtgc ggcactcgca gccagcgtac ggtgatatgc gtgccttccg gcgtatgatt
    51781 cacggcgtta tagaccaggt tcgaaatcgc actgcgtagc tgatcttcgt tgccagacac
    51841 cttgaggccg ttatctatct caaaggtaaa tgtctgtttt ttctgactca gagtctgagc
    51901 ctcgcgctca acaacgcgca gcatcatcgg cacatcaacc ttttcattga gcaattgcgt
    51961 cggcgcggct tcaattttcg acagcgttag caactgcttc accagccctt ccatccgctg
    52021 ggtctgctcg cgcatggtgt gcaacgcttt ttcgcgtacc gcgccttcca gcggctgctc
    52081 atccatcatc tccaggtaac cctgtaacac ggtcaatggc gtacgtaact catggctcac
    52141 gttggcaaaa aagttacgcc gcgccccttc cagttgatgc atttgcgtga catcacgcgc
    52201 caccatcagc aactgtttgt gggtataagg catgacgcga atttccagat gccgcccggt
    52261 gttgagcacc agattgagcg ggcgagaaaa atcacgcgtt ttcagatatt gcgtaaactc
    52321 cgggtaacgc agcaggttaa ggatgttctg cccgttatct tccggccagc gcaaaccaag
    52381 aatttgttgc gccagaccgt tacaccagaa aataccgccc tcttccgtgg tcagcaccac
    52441 tgcgtcgggc agcgactccg cgccgctacg aaagcgttta atcagattgc ccagttcacg
    52501 gcggcgtttt ttatttcgca gctgcatctg gtgtaagccg tatagtagcg gttcccagct
    52561 accacgcccc ggtggcgggg tcatactacg atccacccac agccaccatg aaaggcgcaa
    52621 taaattccag aaatgccaga taagcagtcc cgttaccgat gccagcaaaa accagggcag
    52681 gtaaccaaaa aatgcaccca ggatgaaagc cgggaggcag caaagtagca gctccagcac
    52741 cagccttttc cacgacagcc gttccagcac gtaagatact ccagttaaga aatcataagc
    52801 cctgctctgc gtccgatgag caaggcgtta aaagcgggtt gaaaaacgat atcctgtacc
    52861 gcgcacggtc tgcaccatgc ggtcatgccc gccgggctcc agtgctttac gcaggcgacg
    52921 aatgtggaca tcgaccgtgc ggtcttccac atacacgtta gttccccaga cgtggtttaa
    52981 cagctgctcg cggctgtaca cgcgctcagg atgcgtcata aaaaagtgca gcagtttaaa
    53041 ttctgtcggc cccatctcca gcggctcttc gcccgccatc actcggtgag atgtcgggtc
    53101 gagacttaat ccctgcatct caatcacctc ttccaccgcc attggcgaaa tacggcgcat
    53161 taccgctttg attcgcgcca ccagctcctt cggcgaaaac ggcttggtga tatagtcatc
    53221 cgcgccggtt tcaaggccgc gcacgcgatc ttcttcttcc cctctggcgg tcaacatcac
    53281 cactggaata tcccgggtca tcgactcgcg cttgaggtgt ttgatgaact ggataccgga
    53341 gccgccaggt aacatccagt caaggagaat taaatccggc cagggttcat tcagttgatt
    53401 cacagcactg tcataatctt ccgcttcgac cggctgaaag ccattttgtt cgagcacgaa
    53461 gcagaccatt tcgcgaattg gagcttcatc ttctacgacc agaatacgtc tcgccatgat
    53521 ttgccctgtt gtaataaata ggttgcgatc attaatgcga cgtcattatg cgtcagattt
    53581 atgacagatt tatgaaaagc tcgtcgcaca tatcttcagg ttattgattt ccgtggcgca
    53641 gaaaaaagca aatggcacat ctgtttgggt ataatcgcgc ccatgctttt tcgccaggga
    53701 accgttatgc gcatccttca cacctcagac tggcatctcg gccagaactt ctacagtaaa
    53761 agccgcgaag ctgaacatca ggcttttctt gactggctgc tggagacagc acaaacccat
    53821 caggtggatg cgattattgt tgccggtgat gttttcgata ccggctcgcc gcccagttac
    53881 gcccgcacgt tatacaaccg ttttgttgtc aatttacagc aaactggctg tcatctggtg
    53941 gtactggcag gaaaccatga ctcggtcgcc acgctgaatg aatcgcgcga tatcatggcg
    54001 ttcctcaata ctaccgtggt cgccagcgcc ggacatgcgc cgcaaatctt gcctcgtcgc
    54061 gacgggacgc caggcgcagt gctgtgcccc attccgtttt tacgtccacg tgacattatt
    54121 accagccagg cggggcttaa cggtattgaa aaacagcagc atttactggc agcgattacc
    54181 gattattacc aacaacacta tgccgatgcc tgcaaactgc gcggcgatca gcctctgccc
    54241 atcatcgcca cgggacattt aacgaccgtg ggggccagta aaagtgacgc cgtgcgtgac
    54301 atttatattg gcacgctgga cgcgtttccg gcacaaaact ttccaccagc cgactacatc
    54361 gcgctcgggc atattcaccg cgcacagatt attggcggca tggaacatgt tcgctattgc
    54421 ggctccccca ttccactgag ttttgatgaa tgcggtaaga gtaaatatgt ccatctggtg
    54481 acattttcaa acggcaaatt agagagcgtg gaaaacctga acgtaccggt aacgcaaccc
    54541 atggcagtgc tgaaaggcga tctggcgtcg attaccgcac agctggaaca gtggcgcgat
    54601 gtatcgcagg agccacctgt ctggctggat atcgaaatca ctactgatga gtatctgcat
    54661 gatattcagc gcaaaatcca ggcattaacc gaatcattgc ctgtcgaagt attgctggta
    54721 cgtcggagtc gtgaacagcg cgagcgtgtg ttagccagcc aacagcgtga aaccctcagc
    54781 gaactcagcg tcgaagaggt gttcaatcgc cgtctggcac tggaagaact ggatgaatcg
    54841 cagcagcaac gtctgcagca tcttttcacc acgacgttgc ataccctcgc cggagaacac
    54901 gaagcatgaa aattctcagc ctgcgcctga aaaacctgaa ctcattaaaa ggcgaatgga
    54961 agattgattt cacccgcgag ccgttcgcca gcaacgggct gtttgctatt accggcccaa
    55021 caggtgcggg gaaaaccacc ctgctggacg ccatttgtct ggcgctgtat catgaaactc
    55081 cgcgtctctc taacgtttca caatcgcaaa atgatctcat gacccgcgat accgccgaat
    55141 gtctggcgga ggtggagttt gaagtgaaag gtgaagcgta ccgtgcattc tggagccaga
    55201 atcgggcgcg taaccaaccc gacggtaatt tgcaggtgcc acgcgtagag ctggcgcgct
    55261 gcgccgacgg caaaattctc gccgacaaag tgaaagataa gctggaactg acagcgacgt
    55321 taaccgggct ggattacggg cgcttcaccc gttcgatgct gctttcgcag gggcaatttg
    55381 ctgccttcct gaatgccaaa cccaaagaac gcgcggaatt gctcgaggag ttaaccggca
    55441 ctgaaatcta cgggcaaatc tcggcgatgg tttttgagca gcacaaatcg gcccgcacag
    55501 agctggagaa gctgcaagcg caggccagcg gcgtcgcgtt gctcacgccg gaacaagtgc
    55561 aatcgctgac agcgagtttg caggtactta ctgacgaaga aaaacagtta cttaccgcgc
    55621 agcagcaaga acaacaatcg ctaaactggt taacgcgtca ggacgaattg cagcaagaag
    55681 ccagccgccg tcagcaggcc ttgcaacagg tgttagccga agaagaaaaa gcgcaacctc
    55741 aactggcggc gcttagtctg gcacaaccgg cacgaaatct tcgtccacac tgggaacgca
    55801 tcgcagaaca cagcgcggcg ctggcgcata ttcgccagcg gattgaagaa gtaaatactc
    55861 gcttacagag cacaatggcg cttcgcgcga gcattcgcca ccacgcggcg aagcagtcag
    55921 cagaattaca gcagcagcaa caaagcctga atacctggtt acaggaacac gaccgcttcc
    55981 gtcagtggaa caacgaactg gcgggttggc gtgcgcagtt ctcccaacaa accagcgatc
    56041 gcgagcatct gcggcaatgg cagcaacagt taacccatgc tgagcaaaaa cttaatgcgc
    56101 ttgcggcgat cacgttgacg ttaaccgccg atgaagttgc taccgccctg gcgcaacatg
    56161 ctgagcaacg cccactgcgt cagcgcctgg tcgcgctgca tgggcagatt gttccccaac
    56221 aaaaacgtct ggcgcagtta atggtcacta tccagaatgt cactctggaa cagacgcaac
    56281 gtaatgtcgc acttaacgaa atgcgccagc gttataaaga aaagacgcag caacttgccg
    56341 atgtgaaaac catttgcgag caggaagcgc gcatcaaaac gctggaagcc cagcgtgcac
    56401 agttacaggc gggtcagcct tgtccacttt gtggttccac cagccatccg gcggtcgagg
    56461 cgtatcaggc gctggagcct ggcgttaatc agtctcgatt actggcgctg gaaaacgaag
    56521 ttaaaaagct cggcgaagaa ggtgcggcgc tgcgcgggca actggatgca ttaacgaagc
    56581 agcttcagcg cgatgaaaac gaagcgcaaa gcctccgaca agatgagcaa gcacttactc
    56641 aacaatggca agccgtcacg gccagcctca atatcacctt gcagccacag gacgatattc
    56701 aaccgtggct tgatgcacaa gatgagcacg aacgccagct gcggttactc agccaacgac
    56761 atgaattaca agggcagatt gccgcgcata atcagcaaat tatccagtat caacagcaaa
    56821 ttgaacaacg ccagcagcag ctcttaacgg cattggcggg ttatgcactg acattgccac
    56881 aggaagatga agaagagagc tggttggcga cacgtcagca agaagcgcag agctggcagc
    56941 aacgccagaa cgaattaacc gcgctgcaaa accgtattca gcagctgacg ccgattctgg
    57001 aaacgttgcc gcaaagtgat gatctcccgc acagcgaaga aactgtggcg ctggataact
    57061 ggcggcaggt tcatgaacaa tgtctcgcat tacacagcca gcagcagaca ttacagcaac
    57121 aggatgttct ggcggcgcaa agtctgcaaa aagcccaggc gcagtttgac accgcgctac
    57181 aggccagcgt ctttgacgat cagcaggcgt tccttgcggc gctaatggat gaacaaacac
    57241 taacgcagct ggaacagctc aagcagaatc tggaaaacca gcgccgtcag gcgcaaacgc
    57301 tggtcactca gacagcagaa acgctggcac agcatcaaca acaccgacct gacgggttgg
    57361 ctctcactgt gacggtggag cagattcagc aagagttagc gcaaactcac caaaagttgc
    57421 gtgaaaacac gacgagtcaa ggcgagattc gccagcagct gaagcaggat gcagataacc
    57481 gtcagcaaca acaaacctta atgcagcaaa ttgctcaaat gacgcagcag gttgaggact
    57541 ggggatatct gaattcgcta ataggttcca aagagggcga taaattccgc aagtttgccc
    57601 aggggctgac gctggataat ttagtccatc tcgctaatca gcaacttacc cggctgcacg
    57661 ggcgctatct gttacagcgc aaagccagcg agacgcagga agtcgaggtt gttgatacct
    57721 ggcaggcaga tgcggtacgc gatacccgta ccctttccgg cggcgaaagt ttcctcgtta
    57781 gtctggcgct ggcgctggcg ctttcggatc tggtcagcca taaaacacgt attgactcgc
    57841 tgttccttga tgaaggtttt ggcacgctgg atagcgaaac gctggatacc gcccttgatg
    57901 cgctggatgc cctgaacgcc agtggcaaaa ccatcggtgt gattagccac gtagaagcga
    57961 tgaaagagcg tattccggtg cagatcaaag tgaaaaagat caacggccta ggctacagca
    58021 aactggaaag tacgtttgca gtgaaataac tattcagcag gataatgaat acagaggggc
    58081 gaattatctc ttggccttgc tggtcgttat cctgcaagct atcactttat tggctacggt
    58141 gattggtagc cgttctggta gttgtgatgg tggtatgaaa aaagtcattt tatctttggc
    58201 tctgggcacg tttggtttgg ggatggccga atttggcatt atgggcgtgc tcacggagct
    58261 ggcgcataac gtaggaattt cgattcctgc tgccgggcat atgatctcgt attatgcgct
    58321 gggagtggtg gtcggtgcgc caatcatcgc actcttttcc agccgctact cactcaaaca
    58381 tatcttgttg tttctggtgg cgttgtgcgt cattggcaac gccatgttca cgctctcttc
    58441 gtcttacctg atgcttgcca ttggtcgcct ggtttccggc tttccgcatg gcgcattttt
    58501 tggcgtcggc gcgatcgtgt tatcaaaaat tatcacaccc ggaaaagtca ccgccgccgt
    58561 ggcggggatg gtttccggga tgacagtcgc caatttgctg ggcattccgc tgggaacgta
    58621 tttaagtcag gaatttagct ggcgttacac ctttttattg atcgctgttt ttaatattgc
    58681 ggtgatggca tcggtctatt tttgggtgcc ggatattcgc gacgaggcga aaggaaagct
    58741 gcgcgaacaa tttcactttt tacgcagccc ggccccgtgg ttaattttcg ccgccacgat
    58801 gtttggcaac gcaggtgtgt ttgcctggtt cagctacgta aagccataca tgatgtttat
    58861 ttccggtttt tcggaaacgg cgatgacctt tattatgatg ttagttgggc tagggatggt
    58921 gttgggaaat atgctaagtg gcaggatttc aggacgttat tcaccactgc gcattgcagc
    58981 agtgactgac tttataattg tactggcact gctgatgctc tttttctgct gcggcatgaa
    59041 aacaacgtcg cttatttttg cttttatttg ttgcgcggga ttatttgccc tttcagcacc
    59101 tctgcaaata ttgttactac aaaacgccaa aggcggagag ttattaggtg ccgcaggtgg
    59161 gcaaatagcg tttaacctcg gtagcgccgt cggcgcatat tgcggaggta tgatgctgac
    59221 gctggggctg gcatataatt acgtggcgct gcctgccgcc ctgctttcgt ttgctgcgat
    59281 gtcatcgttg ctgctgtatg gtcgctataa gcgccagcaa gcggcggata gtccggtgct
    59341 ggcgaaacca ctggggtaga ttactgtcac ggtgcgcgtt gcttattgcc ggatgcggcg
    59401 tgaacgcctt atccgcccta cgcggttctg gcacattttg taggcctgat aagacgcggc
    59461 aagcgtcgca tcaggcatga tgcgccaatt gcctacgttt tttactcttg tggccataac
    59521 cacgcagcgc cgcgtacgcc gctggaatca ccgtgcttcg ccttacgcac cggcgtttca
    59581 cattcgccgc cgaagacaaa ttgtttaatc aactgcggaa ccgtttgata taaacggtct
    59641 acattgctca tcccgccccc caggacaatc acatccggat cgagaatatt cacgacatgt
    59701 gccagcgatt ttgccagccg cagctcgtag cgacgcaatg ccagttccgc taccggatcg
    59761 ctttcttcaa ccaggctgat aatttcactg cctttcagcg catgtccgct caaacgacga
    59821 taatccgtcg cgaatccagt gcccgaaata aaggtttcaa tacaaccttg tttaccgcaa
    59881 taacaaggga cttcctcgcg ataacgcagt tcgtcttcgt ccatccacgg tagcggattg
    59941 tgtccccact cacctgccgt gccattgccg ccgatatgcg cccgcccatt gaatgccacg
    60001 cccgcgccgc atcccgtgcc gataatcacg gcaaataccg tctgcgctcc cgctgccgcg
    60061 ccatctaccg cttctgaaac cgccagacag ttggcgtcat ttgccagccg cacttcccgc
    60121 tgcaaccgcg cgcttaagtc tttatcgaat ggctgaccgt tgagccaggt tgaattggca
    60181 ttcttcacca caccggtgta gggcgaaatc gaacctggaa tgcccatacc taccgttccg
    60241 cgctgccctg tcgcctgctc cgccatatca accaacgtgg cgatcgtttc aatagtttgc
    60301 cggtaatcat cacgcggcgt gggcagacga tggcggtaca actgctcccc tgcatcgccc
    60361 agtgcaatca cttcagtttt ggtgccgcct aaatcgatac ctatacgcac ggtactctcc
    60421 ttattttttt caatatcaat agcgtagaga cggacaaccg gattggcaat gcaaggccgc
    60481 cgacaattcg ttatcatgcc cgctaaattt aacgacaagg ccgtggaaat tatcatgctg
    60541 tggttcaaaa atttaatggt ttaccgtctt agccgcgaga tttcgctgcg tgcagaagag
    60601 atggaaaaac agctagcctc gatggcattt accccatgcg gcagccagga catggcgaag
    60661 atgggctggg ttcctccgat gggatcgcac agcgatgcgt taacgcacgt tgccaatggt
    60721 caaattgtta tctgcgcgcg caaagaagaa aaaatcctcc cgtctccggt gattaaacag
    60781 gcgctggaag cgaaaatcgc caaactggaa gcggaacagg cgcgtaagct gaagaaaacc
    60841 gaaaaagatt cgctgaaaga cgaagtgctg cactctctgc tgccgcgtgc tttcagccgt
    60901 tttagccaga caatgatgtg gatcgacacg gttaacggtt tgattatggt ggactgcgcc
    60961 agtgccaaaa aagcggaaga tacgctggca ttactgcgta aaagcctggg gtcgttaccg
    61021 gttgtaccat tgagcatgga aaacccgatt gaactgacgc tgaccgaatg ggttcgctcc
    61081 ggtagtgcgg cacagggctt ccagctgctt gatgaagccg agctgaaatc gttgctggaa
    61141 gatggcggcg tgatccgcgc gaagaaacaa gatctgacca gcgaagagat caccaatcac
    61201 attgaagccg gaaaagtggt gactaaactg gcgctcgact ggcagcagcg cattcagttt
    61261 gtgatgtgcg acgatggttc gctcaagcgt ctgaagttct gcgacgagct gcgcgatcaa
    61321 aacgaagata tcgaccgtga agatttcgcc cagcgttttg atgccgattt catcctgatg
    61381 actggtgaac tggcagcgtt aattcaaaac ctgattgaag gattaggtgg cgaagcacaa
    61441 cgttaattgc tgattttcct ttaatgccgg atgcgacgcc tgccgcgtct tatccggcgt
    61501 acgaagccac accaggcata taattattcg ctacggcggg caataatttt tagcgcagca
    61561 atattatgcg ttttatgctg taacttgctc catggacgtt gtgtcattgt ttttcctcaa
    61621 gccagtgacg cgcttcactg cgaattttcc gcattgcccc ggttttcact aaatcccgta
    61681 acagcgaaag atcgggttgt tcgacaaata agagtaatga tggatcctct tttaaccacg
    61741 tcgttttcac atcggcagcg agctgtggat tattcatagc tagcgaccga tgccgagact
    61801 cggtcaatgt cgttaacaac gacgccgggg tatgtgcgtt gcgtaatgcc gtttggcgca
    61861 aatgatcatt gcgactttgc agcaatgcac gccaccattc atcatctgat ttatctttta
    61921 tcgtagccag ttccgcaaca atatctggag atcttcactg atatttatat caaccttgat
    61981 gaattatgaa tataccggaa attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta
    62041 aagagaggat atttcagaaa aatgccctcc cgttcaaggg gaaggcgagg aattacagat
    62101 agcggcacag ataagaggtg ggttcggcaa cttgcagatg aaactcactg tgaccgggaa
    62161 cattaaacac cgaaccggct tcatacacct gccagtcggt cgcgtcaggc agtaacacat
    62221 tcagcgcgcc actgattacc gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc
    62281 cttcaaccat aacacccacg ctggcgcgac cagtgctgct gctggaaaag ccgattgatt
    62341 tcactttgcc ggaaaagtac tcattacttt gaagcataaa ctggccctgt ctggtagttg
    62401 aacatctact tcactatagg ggccagaggc gctggctgtc acgcaaaatt acaccagtaa
    62461 ttccgcagcc agccgtgcaa tcaatacgtt agacagcaag acaggaacat cgagctgttt
    62521 ttgcagtaaa tcgcgatgac gttggtgaaa tcctaaacaa tccagcatga tgacatcagc
    62581 cccttttgcc agtaattctt tcccggcatc aatgattttt tgttctgaat catgaatggg
    62641 gttgcccaat gaaaataccg gcgatttctg caaaatttgc catttttgcg cctgaacggt
    62701 cagcatctcc tcaaccggaa cgataacccc cacctgatga tcttcaacaa tagaggaaac
    62761 cagtggaggc aatattcgcg acggctcaag aaagatcgta ttacgcgcag tcatactact
    62821 aatgtttgct gtactcatta ataaaatgac gtcataaccc tgattatcga gcacttcaac
    62881 cacaccttgc aggtctcgct ccactttacg acgcgaaaca tgggccagct ggttgtcatt
    62941 taataatgtg agaatggtgt cttcgcctgc ttctggcgcg tactccgcca tcacttcttc
    63001 acgacttaac ttccccagca agctatgatg ggaaatatta tcttcgtcaa tgtattccgt
    63061 caggagcggc aaaacctcct gcataggtac aatgccgatg gtcaggatcg ccaacgacgc
    63121 actcattgtt catccacctt acttttcttt cgtcgttaat taccgggcaa gtgtgaagca
    63181 ccactgctga cacttacttg tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg
    63241 cataatgtaa agattccaaa tgattccagt aatggattgt tattgtttaa tattctaatt
    63301 attagaaaaa catgaattat gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat
    63361 cccttccgag cagtctgatg cggtcactct ttgtcagggt cctcatagcg ttttttcgca
    63421 tccatcgctt cctgagccgt cggatgttca ctgatcaacg agtctggttt aggatgatcg
    63481 gctctgagtt ggtaccaggt taccgtctgt cctggctttc ctttttcaat cgtcactata
    63541 tatgcttcac gaggataagg cggtttcgtt ggcatcgttc ttccttattt cacgggatga
    63601 acgttaagta taggcgctct aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat
    63661 ttcagaaatc acctggctgg gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg
    63721 atatagcgca tcgcgttctt ccagcacttc ctgaacttct tcgctcagcg gttttcccgt
    63781 taaggttggc cgtaaatctt cttccggtgc agcttgcagt cggttaacca ggactgatac
    63841 tggcgcacac aaataaacca cgatcccgtt attttgcatg aagtgacgat taaattccgt
    63901 cagaataatg ccgccgcctg tagcgataac ggtggatggc gcagttaccg cttccagcgc
    63961 cgccgtttct ctggcgcgaa atcccgccca ctcttccctt tcgacgatct ccgcgaccgt
    64021 catattgagc tgtgattgca accactgatc ggtatcgaca aaccgacggt taagcgaatc
    64081 ggcaagggcc attccgaccg ttgttttacc acagccccga ggcccgatca gaaaaagagg
    64141 ttgtgtcatc gtgggttttc cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga
    64201 atagcgatca tgccatcaaa ctagtacaat ttcgattgta aagaaaaaat tccacttaaa
    64261 gtgaaaatct caatacatcc cttactatac caataaatat tcaagaatga agtgtaaata
    64321 ataaattaca tttagccacg actacgttgc acttccagcc accacttctc cagctccgcg
    64381 gcaaaggcct ggcggtcacg ttgtgaaagg ctatctggtc cgccggtctg gatcccactg
    64441 gcacgtaagg tatccataaa atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg
    64501 taacgttcgc cgcgcggatt aagcgccgct gcgcctttct cgatggcttc agcagccaaa
    64561 ggtatatctg cggtgatcac caaatcgccc gcttcacact gccggacaat ttcgttatcg
    64621 gcaacgtcga aacctgccgc gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt
    64681 aaactctggt ttgctaccag taccagcggc atctgcatac gttccgccgc gcgatacaaa
    64741 atctctttaa ttacattggg acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc
    64801 tttgttgggt aatcctctat tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc
    64861 ttctgccagc gattatcaaa acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa
    64921 aatcggtttt attggctgcg gcaatatggg aaaagccatt ctcggcggtc tgattgccag
    64981 cggtcaggtg cttccagggc aaatctgggt atacaccccc tccccggata aagtcgccgc
    65041 cctgcatgac cagttcggca tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc
    65101 cgacatcatt tttgctgccg ttaaacctgg catcatgatt aaagtgctta gcgaaatcac
    65161 ctccagcctg aataaagact ctctggtcgt ttctattgct gcaggtgtca cgctcgacca
    65221 gcttgcccgc gcgctgggcc atgaccggaa aattatccgc gccatgccga acactcccgc
    65281 gctggttaat gccgggatga cctccgtaac gccaaacgcg ctggtaaccc cagaagatac
    65341 cgctgatgtg ctgaatattt tccgctgctt tggcgaagcg gaagtaattg ctgagccgat
    65401 gatccacccg gtggtcggtg tgagcggttc ttcgccagcc tacgtattta tgtttatcga
    65461 agcgatggcc gacgccgccg tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc
    65521 cgctcaggcg gtaatgggtt ccgcaaaaat ggtgctggaa acgggagaac atccgggggc
    65581 actgaaagat atggtctgct caccgggagg caccaccatt gaagcggtac gcgtactgga
    65641 agagaaaggc ttccgtgccg cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga
    65701 aaaactcagc aaatcctgat gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta
    65761 cgtccggctt tctttgcttt gtaaagcgcc aaatctgccg atttcaacca ctcacgatag
    65821 tgactcattt gtgggttcag cggcgcaacc cccacactaa tccgtaaagt tacctgtggc
    65881 gtattcggca agcgtaacgt gtttagccct tcacgcaccc gtaacatggc ggtaatggcg
    65941 ctctcagctg gcgtaccgga catgattact gcaaactcat cgccgccaaa ccgaccaatc
    66001 acatcgctac cgcgcagggt aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg
    66061 cccacatcat gaccccaggt atcgttgatg ctcttgaaat ggtcgatatc gataatcagt
    66121 aacgttgcat cgcgattatg ccgccgacag ttatcaaatt cattgcgtaa catagtttcc
    66181 caatgacgtc ggttatacac gcccgtcatg ccgtcgcggg tactcatgac ctgcaacctg
    66241 cgtttatgtt ccgccagttt ggttgccgtc tggtagctga cccagccaaa cagcagagga
    66301 taaacgacaa taatgggaag ggagagccac cattccagcg gcgcactatt gaacgacacg
    66361 gtaatgcccg taagctcgag ggtgacaagg caggaaacca ccatcaacac cagacccgcg
    66421 acaaacagac gggggccgcc tgcccccatc aaattcagac acataatcat caacatagcg
    66481 gtggaaggca gcacgtttac gcccattacg cctacccaca ttcccgctaa tactgcatcg
    66541 gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg ccctgctcgc tatctgccag
    66601 gctaaatgcg gccagacgaa cgcccagccg accaacacca gccaccacca gcccggcggc
    66661 gggtgcgaaa ccagcgttga agcaatcggt aagaacatgc cagccaggcc aaccgcacgg
    66721 ggtagtctga cgcgacgggc gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt
    66781 aaaggaggtt cctccccatg cgccgccgct tttttgaaaa agttttcatc attcattatt
    66841 tttgggaaca ttcagaaaca attttcccaa attatagaga cggggctaaa ggcagaagta
    66901 tgaaatttcg ggtgagccac tttcgtagct cacccgttag cactatcagg tatcaggcgg
    66961 ctttcttgag gcaggcactc ataaacttat tacgatcatc acctttcaga gattgttgcg
    67021 ttgcttgatt attacattcg cgcatctttt gctgctgtgg cgtcaaactt ttttcaccag
    67081 gcgcagactt gctgttcttc aggcaatcac tcatgtaggt cttacgagca tcccctttca
    67141 acgcctgcgc cgtcgcctgc tgattacagg atgtcatacg ctgttgttgt ggggttaagg
    67201 ttctctcggc agcgccgacg gtggttaaaa aaaccagacc aaaaagcaag gtaaccagta
    67261 atgttatttt catagcacca tccctcttca tgttttaacc atgagcgtat gcgcccgtga
    67321 tctgccatta agtctggttg ctaacagcaa aaaaaccacc cggcagcgaa aattcactgc
    67381 cgggcgcggt tttatttcag ccccagggcg gctttcatgg tgtagaagag atcggtctgg
    67441 tcggtcagtc caacaacatt ggcggcatgc gggccatacg ccgcaatacg caactgactg
    67501 ccggtatgtt cttgtgaatc ctcttcggag ttcccgtagc tcatcaccat cactgcgcca
    67561 tctttggtat ttagcgcctg ggtgaggccc ggagctttgg tatccggcgc gacaatctgg
    67621 ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt tgccatcctt tttagcgaat
    67681 tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct cgccaatttg cccacaagga
    67741 ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa cttgcaggaa aaatcctttc
    67801 tcatttttac tcaacaattc aatggctttg tcggtcatct gcgccagggt tggtacactg
    67861 tcattacgtt gcggattagg cgtacaggtg actgcgggct tatcgatatt gccgtggtac
    67921 gttgctttcg gtcctagcca gcgcactggc atattgccgt cagcaaacag tccaagcaga
    67981 ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg cagcatcgct gaccaactga
    68041 taaccacgcg cctgtgcctg ttcacgcagc gtttttccct gccattcacc ggcggttgcc
    68101 gtttcagcaa aggtttttgc gccaccgcca agcgtaacgt cggcacgagc gttaagcagc
    68161 tgttcggtaa tcgatccttt tccgcctttt tccagagcgt tacccggaca tttttcactg
    68221 gtcgcgctcg gaccgtagca tttgcgcgag gtcacatgcg ccaccagcgc agcgggcgtg
    68281 gcatcctgca actctgcggt agaaacgtta ccggtcgcca gacctgcggc ttttgccatt
    68341 tccagaatcg ttgggtgatc tttttcgtga atatcgacgc ccagcgcgcc gttataggtt
    68401 ttgacaccgg ttgaccaggc ggttgctgat gcagccgaat cggtgacgta gtccggtttg
    68461 ccggtttttt tattcagcgc atagtgagtg tattgcccgg taagcggtaa ggcatcgata
    68521 cctttaaaaa agccgcccgc accttcggca taattacgtg cggctgtaat ttccgaatcc
    68581 cccatcccat cgccaatcag caaaataata ttttttgcag gtttatcgct aagagaatca
    68641 cgcagagcgg cagtctgatc acccgttaaa cggcgagcac cgccggatgc agtaatatcg
    68701 ccctgagcag cccggttttc cagaacaggc atttctggtg tccgggcttt tgtcacaggg
    68761 gtaaacagta acgataagag tgccagtgca atagtgcttt gtttcacttt attttctcca
    68821 tgtacaaata cattaaaaaa taaaaacaaa gcgactataa gtctcggccg tgacaacttt
    68881 atgacagctg ttgaaaagat taacttttta ctgacgagga tgcttcaata acttctttac
    68941 gtaatcgcgc agcagctccg tatcgtcgtc aggaatgctg gcatcgggct ttacctcgta
    69001 cagcgccccc tctacctgat caatcaagcg ctgttggtca ttttgcgcca tattgcgaag
    69061 cattgcagtg acgataatct ccaaagcttc tacctgcgca cacagttctt tcgactcttc
    69121 ttctttttgg gcaagcttaa ataacaactc agcaatgaga tttttcatgt ctgtatttcc
    69181 ttatccaaag tatggagaag ttaacattat gtttcacaac ttcatagtgt ctggtagtat
    69241 tatttttatg acaaaggaaa tactttgcgc aaatattttt tgtttgatta ccagcgaaac
    69301 gtttctctta tttaaataat tacccacaca aaatataggc ttttaattat caacctttat
    69361 tataaataaa agttatgaaa tatattctct tcgttaactt ataatttaga gaaaaaaact
    69421 caccgcatgg ccatttaagc caggaataaa ttagcttccc gttcatcatt tttgtgatct
    69481 tgcgcacatc tttgagataa cgcactttct tcaccagtac gttgcacgct ttgtcagcag
    69541 aagattgcaa caatctttca gaaggccgcg aatcccccgt tttgtcgtgg taatgtatgc
    69601 actttgtgtt agacgggata ggtttttaag atggaaaaac tgcgggtagg aatcgttttt
    69661 ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg caaaaaacat tgtcgatgcc
    69721 attgataaaa gtcgcttcga cgttgtgctg ctgggcattg ataaacaagg gcaatggcac
    69781 gtcagcgatg ccagcaatta tctgctaaat gcagacgatc ctgcccatat tgcgttacgc
    69841 ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg agcatcagct tatcgacgcg
    69901 caaaacggtc agccgttgcc gacggtggat gtcattttcc cgattgtcca cggtacgctg
    69961 ggcgaagatg gttccttgca gggaatgctg cgggtcgcca atttaccgtt cgtaggttct
    70021 gatgttctgg cttcagcagc ctgtatggat aaagatgtta ccaaacgtct gctgcgcgat
    70081 gccgggctga acattgcgcc atttattacc ctgacgcgcg ccaatcgtca caacattagt
    70141 tttgccgaag tggagtctaa actggggtta ccactgtttg taaaaccggc taatcagggc
    70201 tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt acgcaattgc cgtcgatctg
    70261 gcgttcgaat tcgatcataa agtgatcgtt gagcaaggga tcaaaggtcg tgagatcgaa
    70321 tgcgcagttc tgggcaacga caacccgcaa gccagcacct gtggcgagat cgtactcacc
    70381 agcgacttct atgcctacga caccaagtac attgacgaag atggcgcgaa agtggtagtt
    70441 ccggcagcca ttgcgccaga aatcaacgat aagatccggg cgattgccgt tcaggcttat
    70501 caaacgttgg gatgcgcagg catggcgcgt gtagacgtgt ttttaacccc agaaaacgaa
    70561 gtggtgatca acgagatcaa cacactgcct ggcttcacca acatcagtat gtatccgaag
    70621 ctgtggcaag ccagcggtct gggttacacc gatctgatca cacgtttgat tgagctggcg
    70681 ctggagcgtc acgctgcgga taacgcattg aaaaccacaa tgtaatattt taacgcccga
    70741 taatattctt attgggcgtt ttcagtttct tcatcgcgac gacgaataat cagccccgcc
    70801 agccagaaac taatcaccca cgtcaccaga ccgacggcat aggtctgcca accttttgct
    70861 tcaaatccca gtacccccac cacgccatta agaatgaata taaggccaat ggcgaacgca
    70921 tagtagtgcc agtcacggcg gatttttaca ggcagattca tggcagctcc gttaacgaaa
    70981 aacaacatcc ttgcatattc tgtaacaacc gtctgtggtg tagacaacat tttcgtaatt
    71041 gttaataaat ttaacttaag gttgccattt tgtgactcac aacgaagatt cacgcttcgg
    71101 gaatattccc aacatcaatt taccatgatt ctgatattga caatctaaat gacctgtcag
    71161 acttgccata agccgctggt atcacaccag aagagaagca gatgacttcc ggaggttttt
    71221 atggctgatt tcaccctgtc aaaatcgctg tttagcggaa aatatcgcaa tgcctcttca
    71281 acgcctggca acattgccta tgcgttgttt gtgctgtttt gcttttgggc tggggcgcaa
    71341 ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc gtttaatgca ggtccaggaa
    71401 acaggtcgcc cacgggtgga aattggttta ggtgtcggca ccattttcgg gctgatcccg
    71461 tttttagtag gctgcctcat ttttgcagtg gtggcgctat ggctgcactg gcgacatcgc
    71521 cgccagtaag atttacgttg ccagatacgg cctaaaacgc cttatctggc atgacattcc
    71581 tgtttgatca ctgtttcata caacgcgccc gccgttcgtc tacgcgcttc gcaaaccagg
    71641 ccgtagtcag gttgcgggtg attttcgggc tttccagttg aatgccaggc aacatttcgc
    71701 ggggtagtga tttacccgtt ttcgcttcgg caagttgata aactttcttg tacagcgccg
    71761 tctcctcaaa agagaagctg tcacctttct ctaactgacg gcgaatttcg ctgtcgctca
    71821 tccccagttt tcccgccagt ttgcgcgttg ccagttcggt tttcccgggt tctttactgt
    71881 cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc gctggcctta ctgactgcgt
    71941 tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt aaaatcagca aagcggtata
    72001 tcggtgcgct atagctggcg ggataattca gtaaatggta agtaccaaac cacaacccgc
    72061 cgcggcgact gaagacttcc tgacgaactg taccgtccat tttccacgga taccctttgg
    72121 tatgctgttc ggcaaaagca atgctgacct gcatcggccc accggtgcgc acaggattaa
    72181 gcgaaccaaa cagcgtctgc cccattggta ccatgctgat cagatcgtca aaaatcgcgc
    72241 tcaattgctt ttctgtacgc accgaatcta accgttcgct ataacttttg ccgtttggcg
    72301 atttgatttt tagcgcggta tgaaccagaa aagcaggaat gtgcatccgt tcagcacgac
    72361 ggtcaatctc ttgccaggcg atcttgctta agcccggaac ggccggatct gcctgataat
    72421 ttgactcctg ctgcgccacc gccagcaccg agcagacgtt ttccagcgtc ggtgccagcc
    72481 cctgactttc aaaggtggtg gcgagatctt tcgcccaggc atcgcggtct ttcacgctcg
    72541 caggcatctt ctgccgcacg acgctcgcta catctatcgc tttttcgccc tttttcaacg
    72601 gttgcggagc ctgactacta caccccgcta aaaccagaac cgccaacaat gacagcgaag
    72661 atagatgatt gacgcgcgac attacttctc ctttttagct caaggtatgg gttacttcct
    72721 gaattttgtc accatccagc tcatgttcaa agctgcgcag acgtttgtag atagacatca
    72781 actcaaccag tgtggtccat gagttaatca ggtactggaa agcaccgcga acctgaccaa
    72841 aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt accggcaaca attgacggaa
    72901 acagcaagaa caaaccgaaa acgttatcga cctgcaaata gagaatgcgg gcgatgttga
    72961 aatacatata gtgaaaatag aggcgaaaat agtttttccg tacggcgcta aacagctcgc
    73021 gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc ataaaccagc tctttacggt
    73081 aggcggcctc tactcgctgg tttttaaact ccagccccgg cagtttgatc cctaccactg
    73141 ccagcaatcc ggtccccatc agcgaccaga cgatagcggc aatcaccaga ccatacggaa
    73201 tgtgcccgat aatcggcagc tccggcacat gcgcggagag cgttaccagc accggcagaa
    73261 aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc catattctcc agcgttgaag
    73321 caaaacgcat ggtgtcttcc tgcacacgct gtgcggcacc ttcgatatga cgcagttgtt
    73381 gccagttcgc catgtaatat tcgttcatcg ccgtacgcca gcggaacaca tagtgactga
    73441 caaagaagtt gttcagtaca ctgatcacca ccgcgatcag cgcaatcccc agaaagacgc
    73501 ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg cgaacttagc gcggtttgaa
    73561 tcagatcata gaacggtgca taccaggcgt tgacggcgac cccgacttcc accaaaaacc
    73621 aggtgacgaa gatgatcagt gcagtaccga gtattgacca gtattgccaa cggtgcgggc
    73681 tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac aatgtagtaa gcgtaaaaaa
    73741 tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg gatctgcccg gaagcgccgg
    73801 tgatacgcgc cacccagtca cccccaccgg cttgccagaa gataacggca atcaatgccc
    73861 aaacaaaggc cgagagaaaa aacgttcccg gctttgggaa aaaagactta aacatatgca
    73921 ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat taaccgcatg accgctacaa
    73981 agcgtcagcc aaatgaaacc gcccattgtg acccatagca aggcgcttag ttcgccagcc
    74041 tgaccataag aattgtttcg acggatttca caaaccaaag attgtttaca taagcggatt
    74101 aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg aaaaagatcg tgtttacgcg
    74161 ccgttacgtt tttaacacta ttattgcact aattcgccct ttgcaatcca tcaatgagta
    74221 gtataaatac gctcagttac cttcattcaa tctatggaca ccaccgttga aacgtagtct
    74281 gcttttttct gccgtgctgt gtgcggcgtc attgacctcc gtccatgcgg cgcagccgat
    74341 cactgaaccg gagtttgcct ctgatattgt cgatcgttat gccgatcata ttttttacgg
    74401 cagcggtgcc acggggatgg cgctggttgt tatcgacggt aaccagcgcg tctttcgcag
    74461 ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag ctggattcgg tcgtgcgtat
    74521 tgcttccctc accaagctaa tgaccagtga aatgctggtg aaattgctcg accaggggac
    74581 cgtgaagtta aacgatccgc taagtaaata tgctccgcca ggcgcacgcg tgccaaccta
    74641 caatgggacg cccattacgc tggtcaatct ggcaacccat accagcgcgc tgccccgtga
    74701 acagcccggt ggcgcggcac atcgtccggt gtttgtctgg ccaacgcgcg agcaacgctg
    74761 gaaatacctt tctacggcga agctgaaagc cgcgccaggc agtcaggcgg cgtactctaa
    74821 ccttgcgttc gacctgctgg ctgatgcact ggcgaatgcg tccggcaagc cttataccca
    74881 gttgtttgaa gagcagatta cccgtccatt ggggatgaaa gacaccacct acaccccctc
    74941 accggatcag tgccgccgtt taatggttgc agagcgcggt gccagtccat gcaataacac
    75001 gctggcggca attggcagcg gcggcgtcta ttccacgcct ggcgatatga tgcgctggat
    75061 gcagcagtat ttgtcatctg atttttatca gcgcagtaac caggcagatc gcatgcagac
    75121 gttgatttat cagcgcgcgc agtttacgaa agtgattggc atggatgtcc ccggcaaagc
    75181 ggatgcgctt ggtttaggct gggtatatat ggtcccgaaa gagggtcgtc cggggattat
    75241 tcagaagaca ggaggtggtg gcggtttcat tacctatatg gcgatgatcc cgcagaagaa
    75301 tattggcgcg tttgtggttg tcactcgttc gccgttgacg cgctttaaaa atatgagcga
    75361 tggcatcaat gacctggtga ccgagctaag cgggaataaa ccgctggtta tccccgcgtc
    75421 ctgattaata ctctgaaggc aaacggttga tagcgaccag tttgccttta ttcatttcga
    75481 tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac gacagaacgc gatatgcgtg
    75541 tacgttgatg gatataattc atcacaccaa tacgtgatcg caattcttca ttccagtcaa
    75601 tcattgataa taatgttgcg cggatttgtt cgtaggaatt atgcccaatg agctgcacgt
    75661 cgcgtaattc cagaattcta ttttgccagg ctaaccagta aaaagcgtct cgccagagtt
    75721 gattttgttc aataagcgta atggtttgtt tggctggtag atgatatccc gtacaatttc
    75781 cttctgatat taatttgtat ggtatatcgt ttttcattaa accatcagcc agccccataa
    75841 tataaggagc ctgggtaata ccgataagta cgttttcttc tctacgcaga gagataactc
    75901 cctctaaaat aacgaaagta tcttcgttat tgacatcact ggaaaatata acttgctttt
    75961 cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca tttatcgagc ttaccaaatt
    76021 cctgaagagg tttaactaca gataacattt gcgcgtcctt tgcagtaatg cccgtcaaat
    76081 ccttgacggg cattatttag attaaattac cagaatttct tcggagtgaa gaatattacc
    76141 aggtatattt aacacccacg ttcgcggacc agtcttgatc tacgtcacca ccaccgaggt
    76201 agttagcatc ggtataggcg ctgaagttct tggtgaagct gaactgagta cccagaccaa
    76261 cacgtaccgc agacccttca gtaccgttat cgatggaatc accgttcaca tcgttatcgt
    76321 tgttagagtc gtcgtagacg taagccagtt tgaagtacgg agtcagagcc tggtcttcgc
    76381 tgtaggtgaa ggtataacct gcatctacac ccagttcata acgcatgctg tcgtaagact
    76441 gaccgtcaac tttcatgtcg ttgctcagct ggtagtcatc accagactgg aacagaccag
    76501 aaacgctgcc gtaaggagtc acgtaaccag catcacccag tttgaagtcg taaccggctt
    76561 tcaaaccgaa gccccaagcg tcggagttgg tgctaccgtc aacgtaagta ccgttgctca
    76621 tggttgcaga caggtcgttg ttgaagtgag agtagctcaa gctaccatca acaaagacgt
    76681 tgttcgcgaa gtgagcagaa gagtagatgt aggcagtctg gctgtcttga tccacctgac
    76741 cagaacggtc attcatgtca cctttagcga agcctgcagc cgcaccgacg atccacttag
    76801 cgttgttacc gtcaattttg gtatcaacac cgaccatgat gccgttaaca tcctgatcat
    76861 agttgatggt gccgttgtcg ccgttgaagt taccaccgaa gtagcttacc catgcgccgc
    76921 cgttatcagc caggccatga cgagagttgg tcagacgagt accaacggtg tcttgttcca
    76981 ggttccagat attggtgttc gcagacggga tgctcagcgc catgttagcg tagtcggtca
    77041 gctccatctg ttgcagaaca acggtgttac cgcgctgttc agcctgatag gtgtatgcac
    77101 ccaggtcagc tttgttagca gcagagaagg tcgcgttggt gttgacgtcg tttacgtaga
    77161 taatttcttt atctttgtaa tcagcgatag cgccagcgcc tgttgcgttg tcgatacgaa
    77221 ctttgtagtt accggttgca gcaacaacgt tgtcaacaga gctgttatcc agttcgtcgc
    77281 cgttgttcat gtcaccgtta ccgttgatag tcagatgacc atcagagttc atagcaacaa
    77341 taccgtagcc gtagttagac ttggaagtat cccaggtacg gtcgttcacc aaatcagcgt
    77401 tcagaacata atcgctgctg tggatatcga atacaccaga cactacgttg ccgttatggt
    77461 cagcgatgtt gccgctggtc agttccaaag tatcagtctg gaacggatta tcatcgaact
    77521 ggtctacatt aatatccagt aaaccaccgt tagtcacagt tattgtattt gctaacagcg
    77581 acggagtgta ataaccatta tagatacttg aattgtcatc actagacaac gctacagtac
    77641 tgtagctatc aactgtcaga tgatcagttc caacataacc ttcatctcca atgttcaaag
    77701 aagaaccatt ggtcagagcg atgctatcag aaatcaagct agattcagac acattaacct
    77761 gagagccatt attcacagct aaagtatcaa tgtttgaagc gttaacggta ttccattcag
    77821 aaccattatt cagagtgacg ttaaacacac cgctctggaa tacttgatca tcaatcgtac
    77881 gaccccaagc atttgcttct gttacaacgc ccggatacag gctattacct gtaacatcat
    77941 accagtcagt cgaagcagta gattcatcat caacatttgc tgatgtagta gctgagccta
    78001 cccacttaga accattatcc agagtgatat tcagttcatc aacattaagt gagtcatcag
    78061 cccagccacc attggtgtcc aagtacgcca tcggcattac tgtcatgacc ttgaggatag
    78121 aagtttgcgt tgaaagtact tgcgaaaaca acatcaccca gcagagtaga attgctgaaa
    78181 taaacattgt ttttcatgga gtaatttgaa gccgcactat cactgaaata cagagctaca
    78241 tcattagctt cgccgttacc agagtaatca ctaggttccg cagagttacc atagaaacca
    78301 ctgtcttcca gagtagttac agaacctgaa gtcactgtgg aatcggtaac tgagatattg
    78361 ttatcccaag tatgagattc agtagtgtaa gtattgttta cggtattacc ctgagtcagg
    78421 ctgataccag caacgcggga gttgttagaa atattgatgt tactttcgac atcaagattc
    78481 actgccagcc caaggccaac accaccgttt gcaaacaaac cggcgtattc ttcaccttca
    78541 ccagctgggg tatggaaggt ttcgttagta tatttggtaa cgtcaccgtc cagatatgaa
    78601 tcagtgaagt ataaaccttc ataatcgtca tcaattgtag aattagcaat atttaaagtg
    78661 aatacatcac catcatgcca attatcatca attacacgat cagtaccgta ttcagagaac
    78721 tgatagacat aatgaccatc actgctttca cccataggca gcattgaagt gattgaacca
    78781 tgaatcaggg agttgctgat gttcaactgg tttgttgttc catcagtaaa gttgatccta
    78841 cccacgtaat atggacacag gcctaagcga ggttcttgtt ttcaaattgt tccggactga
    78901 ggccgccaca caaactgtgc cgccgccacc gattgtaatc acattcgata taattaaata
    78961 ccgttgcccg cattatttcc cggctgataa agtgttctcc atggatacat tccactttca
    79021 gcgaatgaaa gaagctttcc acgcaggcat tatcgtagca gcaacctttt gcgctcatac
    79081 ttccacgcag attatgccgc ttcagttgcg cctgataatc tgctgaacag tactggcctc
    79141 cacggtccgt gtgaacgata acgttccggg gcctcttacg ccaccacagc gccatctgca
    79201 gggcatcgca ggccagttgc gccgtcatgc gtggcgacat tgaccagcca ataacggcac
    79261 gtgac
//
LOCUS       DAZGOB010000006        73700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGOB010000006 DAZGOB010000000
VERSION     DAZGOB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.006"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(136..212)
                     /locus_tag="ACHLIF_000400"
     tRNA            complement(136..212)
                     /locus_tag="ACHLIF_000400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(176..178),aa:Arg,seq:acg)
     gene            complement(276..352)
                     /locus_tag="ACHLIF_000401"
     tRNA            complement(276..352)
                     /locus_tag="ACHLIF_000401"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Arg,seq:acg)
     gene            complement(550..626)
                     /locus_tag="ACHLIF_000402"
     tRNA            complement(550..626)
                     /locus_tag="ACHLIF_000402"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(590..592),aa:Arg,seq:acg)
     gene            complement(630..722)
                     /locus_tag="ACHLIF_000403"
     tRNA            complement(630..722)
                     /locus_tag="ACHLIF_000403"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(686..688),aa:Ser,seq:gct)
     gene            complement(1038..1223)
                     /gene="csrA"
                     /locus_tag="ACHLIF_000404"
     CDS             complement(1038..1223)
                     /gene="csrA"
                     /locus_tag="ACHLIF_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO9604474.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1458..4088)
                     /gene="alaS"
                     /locus_tag="ACHLIF_000405"
     CDS             complement(1458..4088)
                     /gene="alaS"
                     /locus_tag="ACHLIF_000405"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO9604475.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4216..4716)
                     /gene="recX"
                     /locus_tag="ACHLIF_000406"
     CDS             complement(4216..4716)
                     /gene="recX"
                     /locus_tag="ACHLIF_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO9604476.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4959..6020)
                     /gene="recA"
                     /locus_tag="ACHLIF_000407"
     CDS             complement(4959..6020)
                     /gene="recA"
                     /locus_tag="ACHLIF_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO9604477.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(6100..6597)
                     /gene="pncC"
                     /locus_tag="ACHLIF_000408"
     CDS             complement(6100..6597)
                     /gene="pncC"
                     /locus_tag="ACHLIF_000408"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO9604478.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6742..7827)
                     /gene="mltB"
                     /locus_tag="ACHLIF_000409"
     CDS             complement(6742..7827)
                     /gene="mltB"
                     /locus_tag="ACHLIF_000409"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO9604479.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYVQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            8083..8646
                     /gene="srlA"
                     /locus_tag="ACHLIF_000410"
                     /gene_synonym="gutA"
     CDS             8083..8646
                     /gene="srlA"
                     /locus_tag="ACHLIF_000410"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO9604480.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8643..9602
                     /gene="srlE"
                     /locus_tag="ACHLIF_000411"
                     /gene_synonym="gutE"
     CDS             8643..9602
                     /gene="srlE"
                     /locus_tag="ACHLIF_000411"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO9604481.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFREGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9613..9984
                     /gene="srlB"
                     /locus_tag="ACHLIF_000412"
                     /gene_synonym="gutB"
     CDS             9613..9984
                     /gene="srlB"
                     /locus_tag="ACHLIF_000412"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO9604482.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9988..10767
                     /gene="srlD"
                     /locus_tag="ACHLIF_000413"
     CDS             9988..10767
                     /gene="srlD"
                     /locus_tag="ACHLIF_000413"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO9604483.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10872..11231
                     /gene="gutM"
                     /locus_tag="ACHLIF_000414"
     CDS             10872..11231
                     /gene="gutM"
                     /locus_tag="ACHLIF_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO9604484.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11298..12071
                     /gene="srlR"
                     /locus_tag="ACHLIF_000415"
                     /gene_synonym="gutR"
     CDS             11298..12071
                     /gene="srlR"
                     /locus_tag="ACHLIF_000415"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO9604485.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            12064..13029
                     /gene="gutQ"
                     /locus_tag="ACHLIF_000416"
     CDS             12064..13029
                     /gene="gutQ"
                     /locus_tag="ACHLIF_000416"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO9604486.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(13026..14540)
                     /gene="norR"
                     /locus_tag="ACHLIF_000417"
     CDS             complement(13026..14540)
                     /gene="norR"
                     /locus_tag="ACHLIF_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO9604487.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14727..16166
                     /gene="norV"
                     /locus_tag="ACHLIF_000418"
     CDS             14727..16166
                     /gene="norV"
                     /locus_tag="ACHLIF_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO9604488.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            16163..17296
                     /gene="norW"
                     /locus_tag="ACHLIF_000419"
     CDS             16163..17296
                     /gene="norW"
                     /locus_tag="ACHLIF_000419"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO9604489.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17522..19774)
                     /gene="hypF"
                     /locus_tag="ACHLIF_000420"
     CDS             complement(17522..19774)
                     /gene="hypF"
                     /locus_tag="ACHLIF_000420"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO9604490.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19927..20454)
                     /gene="hydN"
                     /locus_tag="ACHLIF_000421"
     CDS             complement(19927..20454)
                     /gene="hydN"
                     /locus_tag="ACHLIF_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO9604491.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGVGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20603..21613)
                     /gene="ascG"
                     /locus_tag="ACHLIF_000422"
     CDS             complement(20603..21613)
                     /gene="ascG"
                     /locus_tag="ACHLIF_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO9604492.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGRWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            21873..23330
                     /gene="ascF"
                     /locus_tag="ACHLIF_000423"
     CDS             21873..23330
                     /gene="ascF"
                     /locus_tag="ACHLIF_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO9604493.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            23339..24763
                     /gene="ascB"
                     /locus_tag="ACHLIF_000424"
     CDS             23339..24763
                     /gene="ascB"
                     /locus_tag="ACHLIF_000424"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO9604494.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSITWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24877..25296)
                     /locus_tag="ACHLIF_000425"
     CDS             complement(24877..25296)
                     /locus_tag="ACHLIF_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9604495.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(25293..25604)
                     /locus_tag="ACHLIF_000426"
     CDS             complement(25293..25604)
                     /locus_tag="ACHLIF_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO9604496.1"
                     /translation="MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25778..26521)
                     /locus_tag="ACHLIF_000427"
     CDS             complement(25778..26521)
                     /locus_tag="ACHLIF_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9604497.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(26564..27034)
                     /gene="hycI"
                     /locus_tag="ACHLIF_000428"
     CDS             complement(26564..27034)
                     /gene="hycI"
                     /locus_tag="ACHLIF_000428"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO9604498.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(27027..27437)
                     /gene="hycH"
                     /locus_tag="ACHLIF_000429"
     CDS             complement(27027..27437)
                     /gene="hycH"
                     /locus_tag="ACHLIF_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO9604499.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(27434..28201)
                     /gene="hycG"
                     /locus_tag="ACHLIF_000430"
     CDS             complement(27434..28201)
                     /gene="hycG"
                     /locus_tag="ACHLIF_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO9604500.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(28201..28743)
                     /gene="hycF"
                     /locus_tag="ACHLIF_000431"
     CDS             complement(28201..28743)
                     /gene="hycF"
                     /locus_tag="ACHLIF_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO9604501.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28753..30462)
                     /gene="hycE"
                     /locus_tag="ACHLIF_000432"
     CDS             complement(28753..30462)
                     /gene="hycE"
                     /locus_tag="ACHLIF_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO9604502.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(30480..31403)
                     /gene="hycD"
                     /locus_tag="ACHLIF_000433"
     CDS             complement(30480..31403)
                     /gene="hycD"
                     /locus_tag="ACHLIF_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO9604503.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(31406..33232)
                     /gene="hycC"
                     /locus_tag="ACHLIF_000434"
     CDS             complement(31406..33232)
                     /gene="hycC"
                     /locus_tag="ACHLIF_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO9604504.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(33229..33840)
                     /gene="hycB"
                     /locus_tag="ACHLIF_000435"
     CDS             complement(33229..33840)
                     /gene="hycB"
                     /locus_tag="ACHLIF_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO9604505.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33965..34426)
                     /gene="hycA"
                     /locus_tag="ACHLIF_000436"
     CDS             complement(33965..34426)
                     /gene="hycA"
                     /locus_tag="ACHLIF_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO9604506.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            34638..34988
                     /gene="hypA"
                     /locus_tag="ACHLIF_000437"
     CDS             34638..34988
                     /gene="hypA"
                     /locus_tag="ACHLIF_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO9604507.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34992..35864
                     /gene="hypB"
                     /locus_tag="ACHLIF_000438"
     CDS             34992..35864
                     /gene="hypB"
                     /locus_tag="ACHLIF_000438"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO9604508.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35855..36127
                     /gene="hypC"
                     /locus_tag="ACHLIF_000439"
     CDS             35855..36127
                     /gene="hypC"
                     /locus_tag="ACHLIF_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO9604509.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            36127..37248
                     /gene="hypD"
                     /locus_tag="ACHLIF_000440"
     CDS             36127..37248
                     /gene="hypD"
                     /locus_tag="ACHLIF_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO9604510.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            37245..38255
                     /gene="hypE"
                     /locus_tag="ACHLIF_000441"
     CDS             37245..38255
                     /gene="hypE"
                     /locus_tag="ACHLIF_000441"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO9604511.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            38329..40407
                     /gene="flhA"
                     /locus_tag="ACHLIF_000442"
     CDS             38329..40407
                     /gene="flhA"
                     /locus_tag="ACHLIF_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO9604512.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(40444..40797)
                     /gene="ygbA"
                     /locus_tag="ACHLIF_000443"
     CDS             complement(40444..40797)
                     /gene="ygbA"
                     /locus_tag="ACHLIF_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO9604513.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40874..41008)
                     /locus_tag="ACHLIF_000444"
     CDS             complement(40874..41008)
                     /locus_tag="ACHLIF_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9604514.1"
                     /translation="MIPELIISGEEANCEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            41084..43645
                     /gene="mutS"
                     /locus_tag="ACHLIF_000445"
     CDS             41084..43645
                     /gene="mutS"
                     /locus_tag="ACHLIF_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO9604515.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43751..44407
                     /gene="pphB"
                     /locus_tag="ACHLIF_000446"
     CDS             43751..44407
                     /gene="pphB"
                     /locus_tag="ACHLIF_000446"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO9604516.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(44458..45225)
                     /gene="ygbI"
                     /locus_tag="ACHLIF_000447"
     CDS             complement(44458..45225)
                     /gene="ygbI"
                     /locus_tag="ACHLIF_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO9604517.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            45421..46329
                     /gene="ltnD"
                     /locus_tag="ACHLIF_000448"
     CDS             45421..46329
                     /gene="ltnD"
                     /locus_tag="ACHLIF_000448"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO9604518.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            46326..47588
                     /gene="otnK"
                     /locus_tag="ACHLIF_000449"
     CDS             46326..47588
                     /gene="otnK"
                     /locus_tag="ACHLIF_000449"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO9604519.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            47585..48223
                     /gene="ygbL"
                     /locus_tag="ACHLIF_000450"
     CDS             47585..48223
                     /gene="ygbL"
                     /locus_tag="ACHLIF_000450"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO9604520.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            48228..49004
                     /gene="ygbM"
                     /locus_tag="ACHLIF_000451"
     CDS             48228..49004
                     /gene="ygbM"
                     /locus_tag="ACHLIF_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO9604521.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            49093..50457
                     /gene="ygbN"
                     /locus_tag="ACHLIF_000452"
     CDS             49093..50457
                     /gene="ygbN"
                     /locus_tag="ACHLIF_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO9604522.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(50496..50732)
                     /locus_tag="ACHLIF_000453"
     CDS             complement(50496..50732)
                     /locus_tag="ACHLIF_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO9604523.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTESLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(50743..52170)
                     /locus_tag="ACHLIF_000454"
     CDS             complement(50743..52170)
                     /locus_tag="ACHLIF_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO9604524.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(52170..52763)
                     /locus_tag="ACHLIF_000455"
     CDS             complement(52170..52763)
                     /locus_tag="ACHLIF_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO9604525.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            52910..53317
                     /locus_tag="ACHLIF_000456"
     CDS             52910..53317
                     /locus_tag="ACHLIF_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO9604526.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(53438..54430)
                     /gene="rpoS"
                     /locus_tag="ACHLIF_000457"
     CDS             complement(53438..54430)
                     /gene="rpoS"
                     /locus_tag="ACHLIF_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO9604527.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(54493..55587)
                     /gene="nlpD"
                     /locus_tag="ACHLIF_000458"
     CDS             complement(54493..55587)
                     /gene="nlpD"
                     /locus_tag="ACHLIF_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO9604528.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(55772..56398)
                     /gene="pcm"
                     /locus_tag="ACHLIF_000459"
     CDS             complement(55772..56398)
                     /gene="pcm"
                     /locus_tag="ACHLIF_000459"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO9604529.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(56392..57153)
                     /gene="surE"
                     /locus_tag="ACHLIF_000460"
     CDS             complement(56392..57153)
                     /gene="surE"
                     /locus_tag="ACHLIF_000460"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO9604530.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(57134..58183)
                     /gene="truD"
                     /locus_tag="ACHLIF_000461"
     CDS             complement(57134..58183)
                     /gene="truD"
                     /locus_tag="ACHLIF_000461"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO9604531.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(58180..58659)
                     /gene="ispF"
                     /locus_tag="ACHLIF_000462"
     CDS             complement(58180..58659)
                     /gene="ispF"
                     /locus_tag="ACHLIF_000462"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO9604532.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(58659..59369)
                     /gene="ispD"
                     /locus_tag="ACHLIF_000463"
     CDS             complement(58659..59369)
                     /gene="ispD"
                     /locus_tag="ACHLIF_000463"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO9604533.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(59388..59699)
                     /gene="ftsB"
                     /locus_tag="ACHLIF_000464"
     CDS             complement(59388..59699)
                     /gene="ftsB"
                     /locus_tag="ACHLIF_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO9604534.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(59893..60216)
                     /gene="ygbE"
                     /locus_tag="ACHLIF_000465"
     CDS             complement(59893..60216)
                     /gene="ygbE"
                     /locus_tag="ACHLIF_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO9604535.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(60266..60871)
                     /gene="cysC"
                     /locus_tag="ACHLIF_000466"
     CDS             complement(60266..60871)
                     /gene="cysC"
                     /locus_tag="ACHLIF_000466"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO9604536.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(60871..62298)
                     /gene="cysN"
                     /locus_tag="ACHLIF_000467"
     CDS             complement(60871..62298)
                     /gene="cysN"
                     /locus_tag="ACHLIF_000467"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO9604537.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGS
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(62300..63208)
                     /gene="cysD"
                     /locus_tag="ACHLIF_000468"
     CDS             complement(62300..63208)
                     /gene="cysD"
                     /locus_tag="ACHLIF_000468"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO9604538.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            63460..64497
                     /gene="iap"
                     /locus_tag="ACHLIF_000469"
     CDS             63460..64497
                     /gene="iap"
                     /locus_tag="ACHLIF_000469"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO9604539.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   64579..65095
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=64640..64668
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(65192..65485)
                     /gene="cas2e"
                     /locus_tag="ACHLIF_000470"
     CDS             complement(65192..65485)
                     /gene="cas2e"
                     /locus_tag="ACHLIF_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO9604540.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(65485..66405)
                     /gene="cas1e"
                     /locus_tag="ACHLIF_000471"
     CDS             complement(65485..66405)
                     /gene="cas1e"
                     /locus_tag="ACHLIF_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO9604541.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(66402..67052)
                     /gene="cas6e"
                     /locus_tag="ACHLIF_000472"
     CDS             complement(66402..67052)
                     /gene="cas6e"
                     /locus_tag="ACHLIF_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO9604542.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGPAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(67034..67780)
                     /gene="cas5e"
                     /locus_tag="ACHLIF_000473"
     CDS             complement(67034..67780)
                     /gene="cas5e"
                     /locus_tag="ACHLIF_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO9604543.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(67791..68846)
                     /gene="cas7e"
                     /locus_tag="ACHLIF_000474"
     CDS             complement(67791..68846)
                     /gene="cas7e"
                     /locus_tag="ACHLIF_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO9604544.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(68858..69397)
                     /gene="casB"
                     /locus_tag="ACHLIF_000475"
                     /gene_synonym="cse2"
     CDS             complement(68858..69397)
                     /gene="casB"
                     /locus_tag="ACHLIF_000475"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO9604545.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQNQ"
     gene            complement(69394..70956)
                     /gene="casA"
                     /locus_tag="ACHLIF_000476"
                     /gene_synonym="cse1"
     CDS             complement(69394..70956)
                     /gene="casA"
                     /locus_tag="ACHLIF_000476"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO9604546.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(71054..>73700)
                     /gene="cas3"
                     /locus_tag="ACHLIF_000477"
     CDS             complement(71054..>73700)
                     /gene="cas3"
                     /locus_tag="ACHLIF_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO9604547.1"
                     /translation="DFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCFGLAD
                     YFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEKISHS
                     TLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGQPPDRMDELDNFLP
                     EDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRF
                     FPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVL
                     DLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANAMYDR
                     LVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWF
                     ADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCIL
                     EGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVT
                     KSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDAIKVH
                     RQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQVIEQ
                     SVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWD
                     DSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVM
                     PEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSLWL
                     ATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEM
                     ANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT        17348 a        20182 c        18910 g        17260 t
ORIGIN      
        1 tggtgcatcc gggaggatta ctcgccttgc ggctcgccct tcgggccgtt gctaaagcaa
       61 cgttatcatc cctggtgcct gcggttaatt ccgctacctc ttcgatacct tcattgctga
      121 agatttcgga gaatatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag
      181 ccgagtactc tatccagctg agctacggat gcatcgggaa aatttatttt actgctgata
      241 ttgataccgc tactaaagcc atatcaagta agagatggtg catccgggag gattcgaacc
      301 tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat gcatcgggaa
      361 acttacttta ctgcagattt tttgataccg ctactaaagc cgtatcaagt aagagatggt
      421 gcatccggga ggattactcg ccttgcggct cgcccttcgg gccgttgcta aagcaacgtt
      481 atcctccctg gtgcctgcgg ttataccgcc acctcttcca taccttcatc gctgaaagta
      541 cgtagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc gtagccgagt
      601 actctatcca gctgagctac ggatgcaaat ggcggtgagg cggggattcg aaccccggat
      661 gcagcttttg accgcatact cccttagcag gggagcgcct tcagcctctc ggccacctca
      721 ccacacgcct cttacgagtg cttcgaagaa cttgtttatc gctcatcgtc gctgcgtggc
      781 gcacatatta ctttctgaga cttataagtc aaacaatttt tcctacactt ttatcgtttg
      841 cacacttcac gttcaattag tctgtaaaaa cgtcaaaaag agtgttttat caacagaaaa
      901 atggaggtct gacagatagt agtaatgcaa aaaaatggag acttaagttg aatgaacggg
      961 agtaaagcga aaagactata gagtgaagga gaaattttga gggtgcgtct caccgataaa
     1021 gatgagacgc ggaaagatta gtaactggac tgctgggatt tttcagcctg gatacgctgg
     1081 tagatctctt cacggtgaac agaaacttcc ttcggggcat ttacgccaat acgtacctgg
     1141 ttgcccttta cccctaaaac tgtcacggtg acctcatccc caatcatgag ggtctcacca
     1201 actcgacgag tcagaatcag cattctttgc tccttgaaag attaaaagag tcgggtctct
     1261 ctgtatcccg gcattatcca tcatataacg ccaaaaagta agcaatgaca aacacattac
     1321 atgtaagcag tcatggcatt acattctgtt aaacctaagt ttagccgata tacacaactt
     1381 caacctgact ttatcgttgt cgatagcgtt aatgcgaatg ccgtgaagcg agtccacggc
     1441 attgcctgac gcttatatta ttgcaatttc gcgctgaccc agcctttcac actggctaac
     1501 gctgcaggta aggccgcagc atccgtacca ccggcttgcg ccatgtcagg acgtccacca
     1561 cccttgccgc ccacctgctg agcgaccata ccaatcagtt cccctgcttt cacacgatct
     1621 gtgacgtcct tagatacgcc tgcaatcaga gaaaccttac cttcggctac cgttgccagc
     1681 acgataattg tcgaccccag ctgatttttt aaatcgtcaa ccatggtacg caacattttc
     1741 ggctcaacac cgctaagctc gctaaccaac agcttaacac cattaacatc aattgcctta
     1801 ctggaaagat ttgcgctctc ctgtgcggca gcttgttctt taagctgttg tagttctttt
     1861 tctaactgac gcgtacgttc cagtactgag cgcactttat ccgccagatt attgctatcg
     1921 cctttcagca gatgcgcgac ttcgcttaag cgatcgctgt ctgcatgaac ggtggtgata
     1981 gcgccttctc cggttaccgc ttcgatacga cgaacgcctg cagcagtacc cgattcagag
     2041 atgatgcgga acagaccaat atcaccagtg cggctggcgt gagtaccgcc acacagctcg
     2101 gtggagaaat cgcccatgct cagcacgcgt acacgctcat catacttctc gccgaacagc
     2161 gccatcgcac ctttcgcttt cgccgcttcg agatccatga tgttggtttc gatcggcaaa
     2221 ttgcgacgaa tctgtgcgtt caccaggtct tcgaccgcac gaatctcttc cggtttcatc
     2281 gcttcgttgt gtgagaagtc gaagcgcagc accttgtcgt taaccagtga acctttctgc
     2341 gatacatgag tccccagaac ctggcgcagc gcagcgtgca tcaggtgcgt tgcggagtga
     2401 ttcagacgaa tacgggcgcg acgagcctca tcaacatcag cctgcacagc gtcgcccact
     2461 ttcagagaac ccgcagcaag tttaccgatg tgaccaatcg cctggccgta tttctgcgta
     2521 tcttccaccg cgaaggagaa gttagcgcct ttcagttcgc ctttatcacc aacctgaccg
     2581 ccggattccg catagaatgg tgtttgatcc agcacgacca cagcttcctg gcctgcattg
     2641 attgcatcaa ccgctttacc atcaacaaac agcgcggtca ctttgccgtt cagttccaga
     2701 tggtcatagc ctttaaattc agatgcacta tcaacacgga tcattgcgtt gtaatcggca
     2761 ccaaagccgc tggcttcgcg cgcgcgacga cgctgctctt ccattgctgc ttcaaaacca
     2821 gcttcgtcaa ctttgatgtt gcgctcacga caaacatcag ccgtcaggtc aaccgggaag
     2881 ccataggtgt cgtacagacg gaaagccgtt tcaccatcca gcgtatcacc agaaagtttt
     2941 gccagctctt catccagcaa cgccagaccg cgctccagag tacgggcaaa ctgctcttct
     3001 tcagtcttca gcacctgctc aacctgcgcc tgctggcgtt tcaggtcttc acccgcagag
     3061 cccataacgt cgatcagcgg accaaccagt ttgtagaaga aggtttcttt cgcgccgagc
     3121 atattgccgt gacgcactgc gcgacgaatg atacgacgca gtacataacc acggttttca
     3181 ttggacggca ttacgccatc cgcgatcagg aacgcacaag aacgaatgtg gtcagcgatt
     3241 acgcgcagcg atttattgct cagatcggtc gcgccagtga ctttcgctac cgcctggatc
     3301 aacgtgcgga acaggtcgat gtcatagtta gagttaacgt gttgcagcac cgcagcaata
     3361 cgctccagac ccataccggt atctacagac ggcttcggca gcggttccat cgtgccatct
     3421 gcctggcggt tgaactgcat gaagacgatg ttccagatct caatgtagcg gtcgccgtct
     3481 tcttccggac ttcccggagg accaccccaa atgtggtcgc cgtgatcgta gaagatttcg
     3541 gtgcacgggc cgcacggacc agtgtcaccc atctgccaga agttgtcaga tgcgtatggc
     3601 gcacctttgt tatcgccgat gcgaataata cgttcgcgcg ggatcccgac ttctttttcc
     3661 cagatttcgt aggcttcgtc gtcgctttca tagacggtaa cccacagacg ctctttcggc
     3721 agggcaaacc atttttcgct ggtcagcagt tcccatgcaa actgaatggc atcgtgtttg
     3781 aaatagtcgc cgaagctgaa gttgcccagc atttcgaaga aggtatggtg acgcgcggtg
     3841 taaccgacgt tttccaggtc gttgtgttta ccacccgcac gcacgcagcg ttgggaagtg
     3901 gtagcgcggg aataattacg cttgtcgagc ccaaggaaca catccttgaa ctggttcatc
     3961 ccggcgttgg taaacaacaa agttgggtcg ttatggggta ccagggagct gctggcaact
     4021 acctgatgtc ccttactatg gaaaaagtcg agaaacgcct gacggatctc agcggtgctc
     4081 ttgctcataa ttatcctgaa atcaagctaa cgaaatatcg ccaccagctc cagcgtgtct
     4141 taaccgccgg gctggtaact gaaaagtggg aataagataa gttttcttga ctgggaagta
     4201 aaataccgta tgcgttcagt cggcaaaatt tcgccaaata tcctggatat cttccatcag
     4261 atagccacga tagagcagaa aacgctggat cttaactttt tctgaaaaga cagttggcaa
     4321 aggttcacca tattttcgcg tcgcctgatc gcgcgccagt gcgcaccagt cgatgtcaca
     4381 ttcacgcatc gctttttctg tcgcttcgcg ggaaatacct ttctgattca gttcctgacg
     4441 aatacgtgca ggtccataac ctttgcggct acggctggcg ataaagcgcg caacaaatcg
     4501 gctgtcatcg agatagccat gttcatggca ccaggcaata acgcgctcgt aatcttctgc
     4561 cgtagcatca atctcttctg ggccattttt gcccataatc ggtgccacga gtttacgtcg
     4621 cagttcttgc tcactgtgat cgcgcaccgc cagaatgcgt accgcacgat ccaacaggcg
     4681 agcatatgcc gggcgacggg acgttgattc tgtcatggca tatccttaca acttaaaaaa
     4741 gcaaaagggc cgcagatgcg acccaggttt tgctgataaa cagatttaat agtcaatgag
     4801 tgtgcagaat gttcccgcag gcccaattcg ttagaggatc gaggtgagag taatttttca
     4861 cgccagtgct ggcctgttcc cctcccccta accctctccc caaaggggcg aggggaccgt
     4921 ccgtgcacac attatctatt atcatcaata acgaacgatt aaaaatcttc gttagtttct
     4981 gctacgcctt cgctgtcatc tacagagaaa tccggcgttg agttcgggtt gctcagcagc
     5041 aactcacgta ctttcttctc gatctctttc gcggtttccg ggttatcttt cagccaggca
     5101 gttgcattcg ctttaccctg accgatcttc tcacctttgt agctgtacca cgcgcctgct
     5161 ttctcgatca gcttctcttt tacgcccagg tcaaccagtt cgccgtagaa gttgatacct
     5221 tcgccgtaga ggatctgaaa ttcagcctgt ttaaacggtg cagcgatttt gttcttcacc
     5281 actttcacgc gggtttcgct acccaccacg ttttcgccct ctttcaccgc gccgatacga
     5341 cggatgtcga gacgaacaga ggcgtagaat ttcagcgcgt taccaccggt agtggtttcc
     5401 gggttaccga acatcacacc aattttcata cggatctggt tgatgaagat cagcagcgtg
     5461 ttggactgct tcaggttacc cgccagctta cgcatcgcct ggctcatcat acgtgccgca
     5521 aggcccatgt gagagtcgcc gatttcgcct tcgatttccg ctttcggcgt cagtgccgcc
     5581 acggagtcaa cgacgataac gtctactgcg ccagaacgcg ccagggcgtc acagatttcc
     5641 agtgcctgct cgccggtgtc cggctgggag cacagcaggt tatcgatatc gacgcccagt
     5701 ttacgtgcgt agattgggtc cagcgcgtgt tcagcatcga taaacgcaca ggttttacct
     5761 tcacgctgcg ctgcggcgat cacctgcagc gtcagcgtgg ttttaccgga agattccggt
     5821 ccgtagattt cgacgatacg gcccatcggc agaccacctg ccccaagcgc gatatccagt
     5881 gaaagcgaac cggtagagat ggtttccaca tccatggaac ggtcttcacc caggcgcatg
     5941 atggagcctt taccaaattg tttctcaatc tggcccagtg ctgccgccaa cgctttctgt
     6001 ttgttttcgt cgatagccat ttttactcct gtcatgccgg gtaataccgg atagtcaata
     6061 tgttctgttg aagcaattat actgtatgct catacagtat caagtgtttt gtagaaattg
     6121 ttgccacaag gtctgcaatg catacgcagt agcctgacga cgcaccgcat cacggtcacc
     6181 gctgaagcat tcccgccggg taatgccttc accgcgggca gtggcaaaag caaaccagac
     6241 ggtgccgaca ggcttctctt cactgccgcc atccggcccg gcgataccac taatagacac
     6301 ggcataatca gcacgagccg ctttcagtgc acctatcgcc atttccacca cgacgggttc
     6361 actcaccgcg ccatgctgcg ccagcgtctc ttcgcgtacg ccgatcatct gcgctttggc
     6421 ttcgttactg taggtgacaa atccgcgttc aaaccaggcg gagctaccgg caatatcggt
     6481 aatcactttc gctacccaac caccggtaca agactcggca gttgttacgg ttgcgccacg
     6541 ggctttcagc gcctgcccaa cctgttcact taactgcatc agttcactgt cagtcatcac
     6601 aacctctgtc agtcaaaaat ttatgccgga caagatagca ctttcgtcag gaagatggtg
     6661 ttggcgttgc aattttacga ggaggattca gaaaaaagct gattagccag agggaagctc
     6721 acgcccccct cttgcaaata gttactgcac gcgcgccagc gccacggctt gtcctaactg
     6781 ccagaccgcc attgcgtaat gggtgctgtg gttataacgg gtaatggtgt agaagttcgg
     6841 cagaccgtac cagtactggt aaccggtacc gacatccaga cgcagcaggc tggcttgttg
     6901 atggttgccc agcggctgct gtggcgttaa acctgccgtg gcaagctgcg aaatgctgta
     6961 cctggttttg aagccatttg gcaagcctgg agcctgaccg tttgccatta ccgcgacctg
     7021 atcgcctttc acccagccgt gcgctttgaa atagttcgcc acgctaccga tcgcatcaac
     7081 cggatcccac aggttgatat gcccgtcgcc gctgaaatct accgcatatt gtttgtaaga
     7141 cgacggcata aactgtacgt agcccatcgc cccggcaaag gaacctttca gattgagcgg
     7201 atcgtcctgc tcgtcgcgcg ccatcaacag gaaggtttcc agctcgccag agaaatactc
     7261 cgcgcggcgt gggtagttaa atgacagcgt tgccagcgca tcgaggatgc gagttttccc
     7321 catcacgcgc ccccagcggg tttcaacgcc gataatcccg acgataattt ccggcggtac
     7381 gccatacacc tgccacgcgc gattcaacgc atcttcatac tgattccaga aaaccacacc
     7441 gttctgcacg ttgtccggcg taataaattt tttgcgataa cggagccatg cgccgttcgg
     7501 acctgatggc ggtttcaccg atgtggttgg tgcctggtta tccatcagcc gcagtaccga
     7561 atccagacgc ttcgcctggg agagaatttc ctgcaactgc tgacgatcga aaccgtgttt
     7621 gttcaccatt ttgtcgatga actgctgggc attcgggtta ttagcgaaat cgccgcccat
     7681 ctgcatcaca ttgtgctgcg gctcaagcag gaagccgcca gacggcgttc cggtggtcgt
     7741 ttcagtctca gtaggttttg gcttgctgct acaggcggca agcaacacaa aaaggggaag
     7801 caatgttaca taacgacgct tgaacatgag gggtccattt aacagattca accaggggca
     7861 agtatggtaa agcatcacgc cccacacaag gaagcggtag tcactgcccg atacggactt
     7921 tacataactc aactcattcc cctcgctatc cttttactca aactttcaaa ttaaaatatt
     7981 tatctttcat tttgagatca aaataacact tttaaatctt tcaatctgat tagattaggt
     8041 tgccgtttgg taataaaaca ataaatcctg aaggagagaa caatgataga aaccattact
     8101 catggtgcag agtggtttat cgggctgttc caaaagggcg gagaggtgtt taccgggatg
     8161 gtgaccggca ttcttccgct gttgattagc ctgctggtta tcatgaacgc actgattaat
     8221 tttatcggtc agcatcgtat tgaacgtttt gctcaacgtt gcgccggtaa ccctgtttcc
     8281 cgttacctac tgttaccgtg cattggcacg tttgtctttt gcaatccgat gaccttaagc
     8341 ctgggtcgct ttatgccgga aaagtacaaa cccagctact acgcggcggc ctcttatagc
     8401 tgccactcaa tgaatggcct cttcccccat atcaaccctg gcgaactgtt tgtttatctt
     8461 ggcattgcca gcggtctgac aacgctgaac ctgccacttg gcccactggc ggtgagttat
     8521 ctgctggttg gtctggtcac caatttcttc cgcggctggg tgaccgatct gaccaccgcc
     8581 atttttgaga aaaagatggg cattcaactt gaacaaaaag ttcacctggc aggagcaaca
     8641 tcatgacgca tattcggatc gaaaaaggaa cgggtggctg gggcggcccg cttgagctga
     8701 aagccacgcc gggaaaaaaa atcgtctata tcaccgccgg tacccggcct gcgattgttg
     8761 acaaactggc acagcttact ggctggcagg ctattgacgg atttagagaa ggtgaacccg
     8821 cggaggcgga aattggtgtc gcggtaatcg actgtggcgg cacattacgc tgcggcatct
     8881 atccgaaacg acgtattccc accattaata tccactcgac gggcaagtcc ggtccgctgg
     8941 cgcagtacat tgtggaagat atttatgtct ctggcgtaaa agaagaaaac atcactgtag
     9001 tgggtgatgc gacaccacaa ccctcttccg tgggccgtga ctatgacacc agtaagaaaa
     9061 tcaccgaaca aagcgatggt ttactggcga aggtgggaat gggcatgggg tccgccgttg
     9121 cggtgctgtt tcaatctggt cgtgacacca tcgacactgt attaaaaacc attctgccgt
     9181 ttatggcatt cgtctcggcg ctcattggca tcattatggc ttctggcctt ggtgactgga
     9241 ttgcccacgg tcttgctccg ctggcgagcc atccactggg tctggtcatg ctggcgctca
     9301 tctgctcctt cccactgctt tcacctttcc tcggcccagg cgcagttatc gcacaggtta
     9361 tcggcgtatt gattggcgtg cagattggtc tcggcaatat tccgccgcat ctggctttac
     9421 cggcactgtt tgccatcaac gcgcaggcgg cctgcgactt catcccggtc ggtttgtcgc
     9481 tggcggaagc ccgtcaggac acggttcgcg tcggtgtccc ttctgtactg gtgagccgct
     9541 ttttaaccgg cgcaccgact gtactgatcg cctggtttgt ctccggtttt atctatcaat
     9601 agaggctgaa acatgaccgt tatttatcag accaccatca cccgtatcgg cgcgagtgcc
     9661 actgacgccc tcagcgacca gatgctcatc acctttcgtg aaggcgcgcc agcggacctc
     9721 gaagagtatt gcttcattca ttgccacggc gagttgaaag gtgcactcca tcccggtttg
     9781 caatttacgc tcgggcagca tcgctatccg gtgaccgctg ttggcagcgt ggcggaagac
     9841 aaccttcgcg aactgggtca tgtcaccctg cgcttcgatg gtttaaacga agcggaattt
     9901 ccgggcactg tccatgtggc aggccctgta cccgacgata tcgcgccggg atcggttttg
     9961 aagtttgaat ctgttaagga gtaaaaaatg aatcaggttg ccgttgtcat cggtggtggg
    10021 caaaccttag gcgcgttcct gtgccacggt ctggctgccg aggggtatcg cgtcgcggtt
    10081 gtcgatattc agagcgacaa agccgcaaat gtggcacaag aaattaacgc cgaatatggt
    10141 gaaagtatgg cgtacggttt tggtgctgac gccactagcg agcaaagcgt tctggcgctc
    10201 tctcgtgggg tagatgaaat ctttggtcgc gtggatttgc tggtctatag cgccggaata
    10261 gcaaaagcag cctttatcag cgacttccag ctcggcgatt ttgaccgttc gctacaagtg
    10321 aatctggtgg gttatttcct gtgtgcccgt gaattttcgc gtttgatgat ccgcgacggg
    10381 attcagggtc gcattattca gatcaactcg aaatccggca aagtgggcag caaacacaac
    10441 tctggctaca gcgcagcgaa atttggtggc gtcgggctga ctcaatcact ggcgctggat
    10501 ctggcggagt acggcattac ggtgcattca ctgatgctcg gtaacctgct gaaatcgccg
    10561 atgttccagt cactgttgcc acaatacgcg accaagctgg gtatcaaacc ggatcaagtc
    10621 gagcagtatt acatcgacaa agtaccgctc aaacgcggct gcgattatca agatgtgctg
    10681 aatatgctgc tgttctacgc cagtcctaag gcgtcgtact gcaccggaca gtcgatcaat
    10741 gtcaccggcg gtcaggtgat gttctgatca acagcggaga tccattaagg atctccgtga
    10801 gactatagaa tgcctgatgc gctacgctca tcaggcatac aggacttccg ccactacatt
    10861 aaggaaaagt tatggtatcc gcactcatca ccgtcgccgt tatcgcctgg tgtgcgcaac
    10921 tggccttagg cggctggcaa atttctcgtt ttaaccgtgc cttcgacaca ctatgccagc
    10981 aagggcgggt tggcgtgggc cgttccagcg ggcgctttaa accgcgggtc gtggtcgcca
    11041 tcgcgctgga cgatcagcag cgcatcgtcg acaccttgtt tatgaaagga ctgaccgtct
    11101 tcgcccgacc gcaaaaaatt cccgcaatta ccggtatgca tgcgggtgat ttacagcccg
    11161 atgtgatctt tccccatgat ccactatcac agaatgctct atcattggcg cttaaactga
    11221 aacgtggata atttcgttgt gaatgttact tgcttgcgaa gttatcattt tgaaacctaa
    11281 atcaggtaat cacgcccatg aaacctcgtc agcgtcaggc cgccattctg gagtatctgc
    11341 aaaagcaggg taaatgctcg gttgaagaat tggcgcaata ctttgacacc acaggcacaa
    11401 ccattcgcaa agatctggtc attctggaac atgccggaac cgtcattcgt acttatggcg
    11461 gagtggtgtt gaataaagag gaatccgatc cgcctatcga tcataaaaca ctcatcaaca
    11521 cccacaagaa agagctgatt gcagaagctg ccgttagttt tatccatgat ggcgattcga
    11581 tcattcttga tgctggcagt accgttttgc agatggttcc cctgctctcg cgctttaata
    11641 acatcacggt gatgaccaac agcctgcata tcgtcaatgc gctatccgaa ctggataacg
    11701 aacaaactat cctgatgcca ggcggaacgt ttcgtaaaaa atcggcctca tttcacggac
    11761 agctggcaga gaatgccttc gaacatttca gtttcgataa attgtttatg ggcaccgacg
    11821 gcatcgatct caatgcgggc gtaaccacct tcaacgaggt ttataccgtc agtaaggcaa
    11881 tgtgcaatgc cgcgcgcgaa gtgattttga tggcggactc atcaaagttt ggccgtaaaa
    11941 gccccaacgt agtttgcagt cttgaaagcg tcgataagct gattaccgac gcaggtatcg
    12001 atccggcgtt tcgtcaggcg ctggaagaga aagggatcga tgtgatcata accggagaga
    12061 gcaatgagtg aagcactact gaacgcggga cgtcagacgt taatgctgga gttgcaggaa
    12121 gcaagccgtt taccggaacg tctgggcgat gattttgttc gcgccgccaa tatcatcctg
    12181 cactgcgaag gcaaagtggt ggtttcggga attggcaaat cgggccacat tggtaagaaa
    12241 atcgccgcaa cacttgccag taccggcaca ccggcttttt ttgtccatcc ggcagaagca
    12301 ctgcacggcg atctggggat gattgaaagc cgcgatgtga tgctgtttat ctcttactcc
    12361 ggtggcgcga aggaactgga tctgattatt ccgcgtctgg aagataaatc tatcgcgctg
    12421 ctggcgatga ccggcaaacc gacgtcaccg ctgggcctgg cggcaaaagc ggtgctggat
    12481 atctccgtag aacgcgaagc ctgcccgatg caccttgcgc cgacctccag caccgtcaat
    12541 accctgatga tgggtgacgc gctggcgatg gcggtcatgc aggcgcgcgg atttaatgaa
    12601 gaagattttg cccgctccca cccagccggg gcactgggcg ctcgcttgct gaataaagtg
    12661 catcatctga tgcgccgtga cgatgccatc ccacaggtgg cgttaaccgc cagcgtgatg
    12721 gatgcgatgc tggaactcag ccgcaccggt ctggggctgg tggcggtatg tgacgctcaa
    12781 caacaggtac aaggcgtctt taccgacggc gatttacgtc gctggctggt tggcggcggc
    12841 gcactcacca cgccagttaa tgaagcgatg acgacaggcg gcaccacatt acaggcacaa
    12901 agtcgcgcca tcgacgccaa agagatcctg atgaagcgca aaatcactgc cgcaccggtg
    12961 gtggatgaaa acggcaaact caccggcgca attaacctgc aggatttcta tcaggccggg
    13021 attatttaat ccttcaatcc cagacgtttc gccagccgat gcaggttggc gacgtcggtt
    13081 tccagcatcc gcgcgcaggc agcccagttg tgatgatttt gtgccagtgc ctggcgaatg
    13141 gtttcacgct ggaacgcttc tgtcgcttca cgcaggtttt gcttaacaac gggcaccgcc
    13201 gccgcttctg gcggcggcaa cgtcacctca ggaaaagcaa aatgttgtgc ctcaagaatc
    13261 acttcatcgc cgctgcgagt ggctctcgcc agcactaccg cccgatgaat agcatgttcc
    13321 agttcgcgca cgttccccgg aaaacgatag tgttgcagta aatttcgcgc tccggcactt
    13381 aataccacgc gggagagccc cagccgcaaa cgacactgct cgcagaaata ccccgccagc
    13441 agaatgacat catcgccccg ctcacgcagc ggcggcaccg aaagtggaaa cacgctcagg
    13501 cgatgaaaca aatcggcgcg gaatcgccct gccagcacct cttcgcgtaa gtcgcggtta
    13561 gtcgccgcca gcacgcgcac atcgacccgc aaactacggt catcgccaac gcgctgaata
    13621 tcgccatact gcaacaccct cagcagcttg gcctgcaatg ccaacgacaa ctcgccgatc
    13681 tcatcgagaa acagagtgcc gttatccgcc atttcaaact tcccgctgcg gttactgata
    13741 gcgccagtaa acgctccttt cacatgcccg aacaactcac tttccgccac actttccggc
    13801 agtgcggcac agttgagata gaccagcgga ttcaccgccc gtggcgaggc ttcatgaatc
    13861 gctttcgcca ccagctcctt accggttccc gtctcaccgc tgatcagaac gttgagatcg
    13921 gacgccgcca caatctcaat ctcttttttc aattgcgtca tgcctggcga caagccaatc
    13981 atctgcgtct gtttcaccgc ttcaaacggc gcggcatcgc ccggcagcat attctggctt
    14041 tccagttgtt caatcagcaa cgcattgctt aacgctcccg ccgccagcgc ggcaatcagc
    14101 cgtaactctt cgtcgctgaa aacatcgaac tgatcgggct gcatcccgtc gagcgtcagt
    14161 gcgccgatca ggttttgccc ggcaaacaat ggcagaccaa cgcaggcgtg aaccttcaga
    14221 ctctcctgcc caggaatcaa accgtcatag ggatcgggca attcgctgtc tgcaggaaag
    14281 cgcaccacgt ccccggcgcg ggcaatcgct tcaagccgtg gatgcccttc cagcgcaaag
    14341 cgtctaccga gtacatcctt tgccagaccg tcgatggcaa gcggaataaa ctgccgcgaa
    14401 tcgtaacgta gcaacgcaga cgcatcgcac tccagcacct gacgtagcgt ggtgatcagg
    14461 cgctgaaaac gatcctgatg accaatccca cgctgcaatt cgatggcgat attcgccagc
    14521 acatcaacgg aaaaactcat ctttgcctca ctgtcaattt gactatagat attgtcatat
    14581 cgaccatttg attgatagtc attttgacta ctcattaatg agcataattt tatttataga
    14641 gtaaaaacaa tcagataaaa aactggcacg caatctgcaa ttagcaagac atctttttag
    14701 aacacgctga ataaattgag gttgctatgt ctattgtggt gaaaaataac attcattggg
    14761 ttggtcaacg tgactgggaa gtgcgtgatt ttcacggcac ggaatataaa acgctgcgcg
    14821 gcagcagtta caacagctac ctcatccgcg aagaaaaaaa cgtgctgatc gacaccgtcg
    14881 accataaatt cagccgcgaa tttgtgcaga acctgcgtaa tgaaatcgat ctggcggata
    14941 tcgattacat cgtgattaac catgcagaag aggaccacgc tggggcgctg accgaactga
    15001 tggcgcaaat tcccgatacg ccgatctact gtactgccaa cgctatcgac tcgataaatg
    15061 gtcatcacca tcatccggag tggaatttta atgtggtgaa aactggcgac acgctggata
    15121 tcggcaacgg caaacagctc atttttgtcg aaacaccaat gctgcactgg ccggacagca
    15181 tgatgactta cctgacaggc gacgcggtgc tgttcagtaa cgatgctttc ggtcagcact
    15241 actgcgacga gcatctgttc aacgatgaag tggatcagac ggagcttttc gagcagtgcc
    15301 agcgttacta cgccaatatc ctgacgccgt tcagccgcct ggtaacgccg aaaattaccg
    15361 agatcctggg ctttaactta ccagtcgata tgatagcgac ctcccacggc gtggtctggc
    15421 gcgataaccc gacgcaaatt gtcgagctgt acctgaagtg ggccgctgat tatcaggaag
    15481 acagaatcac cattttctac gacaccatgt cgaataacac ccgcatgatg gctgacgcta
    15541 tcgcccaggg aattgcggaa accgacccac gcgtggcggt gaaaattttc aacgtcgccc
    15601 gaagcgataa aaacgaaatc ctgaccaatg tcttccgctc aaaaggcgtg ctggtcggca
    15661 cttcgacgat gaataacgtg atgatgccga aaatcgccgg gctggtggag gagatgactg
    15721 gtttacgctt ccgtaacaaa cgcgccagtg ctttcggctc tcacggctgg agcggcggtg
    15781 cggtggatcg tctttccacg cgcctgcagg atgcgggttt cgaaatgtcg cttagcctga
    15841 aagccaaatg gcgaccagac caggacgctc tggagttatg tcgtgaacac ggtcgcgaaa
    15901 tcgcccgtca gtgggcgctc gcgccgctgc cgcagagcac ggtgaatacg gtggttaaag
    15961 aagaaacctc tgccaccacg acggctgacc tcggcccacg gatgcagtgc agcgtctgcc
    16021 agtggattta cgatccggca aaaggcgagc caatgcagga cgttgcgcca ggaacgccgt
    16081 ggagtgaagt tccggataac ttcctctgcc cggaatgctc cctcggcaaa gacgtctttg
    16141 aagaactggc atcggaggca aaatgagtaa cggcattgtg atcattggtt cgggcttcgc
    16201 cgcccgccaa ctggtgaaaa atattcgcaa acaggacgcc actattccat taaccctgat
    16261 tgccgccgac agcatggatg agtacaacaa acctgacctc agccatgtta tcagtcaggg
    16321 gcaacgtgcc gacgacctta cccgccagac ggcgggtgaa tttgccgagc agtttaatct
    16381 gcgcctgttt ccgcacacct gggtgacgga tatcgatgcc gaagcccgtg tggtgaaaag
    16441 ccagaataat cagtggcaat acgacaagct agtgctggca accggtgcca gcgcctttgt
    16501 cccgccagtg cctgggcgtg agttaatgct gacgttaaat agtcagcaag agtatcgcgc
    16561 ctgtgaaacg caactgcggg atgcccgacg cgtgttgatt gtcggtggtg gcttgattgg
    16621 tagcgaactg gcgatggatt tttgtcgtgc aggcaaagcg gtcacgctaa tcgacaacgc
    16681 tgccagtatt ctggcgtcat taatgccacc ggaagtaagc agccgcttgc agcatcggtt
    16741 gacggagatg ggcgttcatc tgttgttgaa atctcagttg caggggctgg aaaaaacaga
    16801 ttctggcatt ctggcaacgc tggaccgcca gcgcagtatc gaagtggatg cggtaattgc
    16861 cgccaccgga ctgcgcccgg aaaccgccct ggcacgacgc gccgggctga cgattaatcg
    16921 cggcgtttgc gtcgatagtt atctgcaaac cagtaatgcc gatatttacg cgctgggcga
    16981 ttgcgcggaa attaacggtc aggtattgcc gttcctccag ccgattcaac ttagcgcgat
    17041 ggtgctggca aaaaatcttc tcggcaataa cacgccactg aaactaccgg cgatgctggt
    17101 gaaaatcaaa acgccggaat taccgctgca tctggcaggc gaaacccagc gtcaggattt
    17161 acgctggcag attaataccg aacgccaggg aatggtggcg cgcggcgttg acgatgctga
    17221 ccagcttcgc gcctttgtgg tcagtgagga tcggatgaaa gaggcatttg gattgttgaa
    17281 aacgttgccg atgtaggtgg gctactgtgc ctaaaatgtc ggatgcgacg ctggcgcgtc
    17341 ttatccaacc tacggggagc gcatgtgttg gtcacatact gcgttcatgt cgcatccacc
    17401 tactgtgccc aaaatgtcgg atgcgacgct ggcgcgtctt atccgaccta cggggacgca
    17461 tgtgtaggcc ggataaggcg tttacgccgc atccggcaat ggtgtccaaa tgcaacacgt
    17521 tttatccgtt ctggacttca cccgctaacc aacgcgccgc agcaataacc ccctgcccca
    17581 gagacaaacc gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg aaatcagcga
    17641 gataatgcgc cagacgtgca cgcagcaaac ggttatgaat aaccccgccg ctaaatacca
    17701 gcgtagtgat accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa ccctgcgcca
    17761 gcgcatcatg aaacgcccac gcgcgttgat taaccggtgc ctgccagttc agccactgct
    17821 gccagaaagt ggcgagatcc agttgattgt ccaccagcgg cattgtcacc ggatgcgtca
    17881 ctccgtggca cgaagctgcg agtgcctcca gagcacaagc cgcttcacct tcataactta
    17941 acgtggctgg cgcacagccc agtgccgccg ccacggcatc gaacaaccgc ccacacgatg
    18001 acgccagcgg cgcgttaatt ccacgctcaa tggcccgcgc cagcacgctc cagttttgct
    18061 gttgcacact tgctgtttca gaataattct gccactccgg cacaaagcgc aggcactgcg
    18121 ccagcaggtt tcgccacggc tgcttcgccg ccaaatcgcc acccggaagc gccactgcag
    18181 gcaagccgcc caggtgctca cattcgcgat agttcacccg cagacactcg ccgccccaca
    18241 aagcgccgtt ctcccccata ccgataccat cgagcgtcaa agcaatgaca tccccgccat
    18301 ccagcggcca ctgatgctct gccagacacg ccgctgcgtg ggcatgatga tgcagcaccg
    18361 tttgcgtcgg catattcatt tcgctcgccc actggctgga gacatagccc ggatgcacgt
    18421 catgcacaac gtattgcgga gtgaaatcgt agatgttttg catcaggcgt aacgcttcgc
    18481 gccactgcat ctggatgcca tcgtcactta aatcgcccag atgctgactc aacaccgctt
    18541 gttcaccgcg caccaggcag aaggtgtttt tcagatccgc gccgagacac agcacaggcg
    18601 gaacattttt aaagcccgga ggcaaagcca gcgcatccgg cacatacccc cgcgaacggc
    18661 gcagcatttc gccgctttcg cgcaccaccg aatcatccat ccgctgcacg atgtcgcggt
    18721 tatgtatcaa gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt tcgttgctga
    18781 tagctggtgg tttaccgctc aggttgccgg aggtcatcac cagcgggcat tgcagttcct
    18841 gtaacagcaa atgctggagc gggttcgcag gcaacattac cccgacttcg ttaaggtcag
    18901 gggcgatatc atcacaaagc tcaggaacgt attttttatc caccagcaca atcggcgcgg
    18961 cgggcgtggt aagcaactgg cgcgcagcgt ctggtaaacc gtcagccact ggcaacatga
    19021 ctgccagcgg tttcgccggg cggtgtttgc gctcccgcag tgtcgccacc gcgttactgt
    19081 tacgtgcatc gcaggcaaga tgaaatccgc caatcccttt gatggcgaca attttgccca
    19141 tttttaactg tgcgatagct gcctgtaatg ccgcctcttg ttccgcatgt tcaccatgac
    19201 ttacccattc aagatgcggg ccacactccg ggcaggccac cggctgggcg tggaagcgac
    19261 gatcgagcgg gtcgcggtac tctttatcac aggctggaca cagcggaaac tccgccatta
    19321 cggtaaacgg gcggtcgtaa ggcatggcgc gaataatggt gaaacgcggg ccgcagtggg
    19381 tacagttgat aaacggataa cgataacgcc gttcgcctgg ggtattcatt tcggcaaggc
    19441 aagcagggca agtagcggca tccgggacaa tttgcgtatt catggcacct cccgcgctct
    19501 ggcggatagt gaactcggtg ggcagttgtg accagataaa cggctcacgc tcgacgctat
    19561 caatacgcgc cagtggcgga cagtgctgat gcaattgaac aagaaacgtt tccgggtctt
    19621 ccagcagccg gacttctacg ccatcgccgt cattacagac atcgccgtga agatttaatt
    19681 gctgtgccag ctgccagaca aacggacgaa aaccgacgcc ctgcactttg ccacgaatac
    19741 gcagttggac accgcaagat gtgttttttg ccattgagtt attcccgcca tcatgaattg
    19801 cgtaacccgt ccagccggac acgacagcgt cgcatccggc agtcacaggt cggcgatacc
    19861 gcccgcttcg tattctacga atatttccgg gaattccttt gatgccagaa cagttctgta
    19921 agatttttag aacatcagcg ccgtacggcg gcgtttttct gcgctcagtt gttcaagttt
    19981 attacgatcg acacaaatta gcgcatgggt cgggcaagcc gccatacacg ccgggccgtc
    20041 ttcacgatgg ttgcacaggt cgcatttatt ggcttcagct ttgtcagccc gtacattcag
    20101 ccccacgccg ctgttgcgaa tcaccggacg taccaccact tccatcgcgc catacgggca
    20161 agccaccacg caggttttgc aaccaataca acgttcctgc atcacatgaa caaacccttt
    20221 atcacggctg atagcaccat tcgggcagac gttagcgcac ggtgcatctt cacactgacg
    20281 gcaaactgtc gccgtggaaa tgttcacacc tttaatgaca tggatacgcg gtaaaaatgt
    20341 ttccggggtc agcgatgcac agtcctgatt ttcctgatga gaaaccacgc acgccacttc
    20401 acaggtacgg caaccaatac atttactcgc gtcagcaatg atgaaacggt tcatcaaatt
    20461 ctccagcaat gacagttaat gcgccgatac attcacaaat catgccagtt tttaatttac
    20521 tgttatttaa ggaaattaat ttctgtaatg caggaaaaac gatgtcatcg acactagtga
    20581 cgatgacatg tgatgacaat gtttatcgcg aaagggcaat gagtgagtcg cggcagatca
    20641 attttccgct gaaggttttc ggcggtgaga aatccccgcc atcgagcata aaaatcagcc
    20701 gtccaataat ttcctgaatc atctcagtca ccggaatttt tacgctggag agcgccggaa
    20761 cgatataggg ggcaattgcg atatcatcga atccgataac tgacacctgc tctggcaccg
    20821 ctacgccgcg ctcgtgcaac gctttcatcg cacctatcgc catatcgtcg ttactggcaa
    20881 ctaacgcgct aaatttagcc ccacgttcga gcaacatttc taccccttcg gccccgctgg
    20941 caggcgtcca tctaccgtta gcgataagtt tttcattgag cgcaataccg tgctgcgcca
    21001 gcgcgtcttt atatccggca agacgttcaa cgctggtggg agaatccatc gaaccggtaa
    21061 ggaaagcaat ctcctgatgt ccggcgttta tcaactctgc cacggcgtta aaactggtct
    21121 gtttatgatc gcaccagacg ctatggctgc tgtttttgcg caggcggcga ttaagcacca
    21181 ttatcggctg actgtgcgcg tcaatgatgt catcgatctc atccacgctt aaaaaacgcg
    21241 ggtaaatcat aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt
    21301 cttcggcgct gtgtttacca tctgccaata gcaactgccg ccctttctct tccgccattc
    21361 gcgcggcatg aaacagtaat tcactaaaat aaatgccgtg gtaaagcgtg ttggtcacta
    21421 ccagccccag cgtctgagta ctcttcgccg acaaattgcg cgccagcaag tttgggcggt
    21481 aaccgctctc ttctaccgcc tgaaacacgc gatctttagt ctcctggctg acgtagccat
    21541 tacctgaaag cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca
    21601 gcatcgtcgt catcattttc atccctttac acgcaatcaa cgcagtgtac tgcaccgttt
    21661 gccgattgtc cttgcgcaat cggcgggaaa aatattcagg tgaccggttt cacaaatata
    21721 aaaaatgaac aattaactct cttgcttatt tagtgacaac tattcatgat tttgtgaaac
    21781 cggtttccta attccgtttc agcatcggca tttttccgtt acgtcgactg ataacaacta
    21841 catctaccct actgataaca ggataaaatc cgatggccaa aaattatgcg gcgctggcac
    21901 gctcggtgat agcggcactg ggcggcgttg ataacatctc ggcggtcacg cactgtatga
    21961 cgcgtttgcg ctttgttatc aaagatgacc agcttatcga cagcccgacg ttaaaaacca
    22021 tccccggcgt gctcggcgtg gtacgtagtg acaaccagtg tcaggtgatt atcggcaata
    22081 ccgtttcaca agcctttcag gaagtcgtca gcctactgcc gggagatatg cagcccgcac
    22141 agcccgtggg taaacccaaa ctcacgctgc gtcgcattgg tgcagggatc ctcgatgcgc
    22201 tgatcggcac catgtcaccg ctgatcccgg cgattatcgg cggatcgatg gtcaaactgc
    22261 tggcgatgat cctcgagatg agcggcgtgc tgacaaaagg atcgccgacc ttaaccattc
    22321 tgaatgtgat tggtgacggt gctttcttct tcctgccgct gatggtcgcg gcatctgccg
    22381 ccatcaaatt taaaaccaat atgtcgctgg cgattgccat cgcgggtgtg ctggtacatc
    22441 cgagttttat tgaactgatg gcgaaagcgg cccagggtga acatgtcgaa tttgccctga
    22501 ttccggtcac cgcggtgaaa tacacctaca cagtgatccc ggcgctggtg atgacctggt
    22561 gcctgtcata tatcgaacgc tgggtggatc gcattacacc ggcggtgacc aaaaacttcc
    22621 tcaagccaat gctgattgtg ttgattgccg caccgctggc aatcctgctg attggcccga
    22681 ttggtatctg gatcggtagc gccatttcgg cgctggttta caccattcat ggttatctgg
    22741 gctggctttc agtcgccatt atgggcgcgc tgtggcctct gctggtaatg accgggatgc
    22801 accgcgtctt tacgccaacc atcattcaga ccattgccga aaccggcaaa gaagggatgg
    22861 tcatgccgtc agagatcggc gctaacctgt cgctgggcgg ttcatcactg gcggtggcgt
    22921 ggaaaacgaa aaacccggaa ctgcgccaga cggcgctggc tgcggcggca tcagccatta
    22981 tggcggggat ttccgaaccg gcgttatacg gcgtggcgat ccgcctgaaa cgtccgctta
    23041 tcgccagtct tatcagcggc tttatttgcg gcgcggttgc cggtatggcg gggcttgcca
    23101 gccactcaat ggcagcgccg gggctattta ccagcgtgca gttcttcgat ccggcgaatc
    23161 caatgagcat cgtctgggtg ttcgcggtca tggcgctggc ggtcatgctg tcgtttatcc
    23221 tcacactgtt gctcggcttt gaggatattc ctgttgagga agcgactgcc gaggcgcgaa
    23281 agcatcagag cgtacaaccg accgtcgcca aagaagtaag tcttaattga ggatgaaaat
    23341 gtcagtattt ccagaaggtt ttttatgggg cggcgcgctt gccgccaacc agtctgaagg
    23401 tgcgttccgt gaaggtggca aagggctgac cactgtcgat atgatcccac acggcgagca
    23461 tcgaatggcg gtgaaactgg ggctggaaaa acgttttcag ttgcgcgatg acgagtttta
    23521 tcccagccat gaggcgacgg atttttatca tcgttataaa gaagatatcg ccctgatggc
    23581 agagatggga ttcaaggttt tccgtacctc aattacctgg agccgtctct ttccgcaggg
    23641 cgatgaaatc acgcccaatc agcagggcat tgctttttat cgttctgtct ttgaagagtg
    23701 taaaaagtac ggtatcgaac cgctggtcac gttgtgccac ttcgatgtgc cgatgcatct
    23761 ggtcaccgaa tatggctcct ggcgtaaccg caagctggtg gagtttttca gccgctacgc
    23821 ccggacctgc tttgaagcat ttgatggtct ggtgaaatac tggcttacct tcaatgaaat
    23881 caacattatg ttgcatagcc cgttctccgg cgcgggtctg gtgtttgaag aaggtgaaaa
    23941 tcaggatcag gtgaaatatc aggccgcgca tcaccagctg gttgccagtg cgctagccac
    24001 caaaatcgcc catgaggtta acccgcaaaa tcaggtgggc tgtatgctgg cgggcggtaa
    24061 cttctaccct tacagttgca agccggaaga tgtctgggcg gcgctggaga aagaccggga
    24121 aaacctgttt tttatcgatg tgcaggcgcg gggcgcgtat ccggcttact ctgcccgcgt
    24181 attccgcgaa aaaggggtaa ccatcgacaa agcaccgggc gatgatgaaa tcctgaaaaa
    24241 caccgtcgat tttgtctctt tcagctatta cgcctcgcgc tgcgcctcgg cggagatgaa
    24301 cgccaacaat agcagtgcgg cgaacgtagt gaaatcgctg cgtaacccgt atctgcaagt
    24361 gagcgactgg ggctggggta ttgatccact cggtctgcgt atcaccatga acatgatgta
    24421 cgaccgttat cagaagccgc tgtttctggt ggaaaacggc ctcggcgcaa aagatgaact
    24481 tgctgccaat ggcgagatta acgatgacta tcgcatcagc tatttacgcg aacatatccg
    24541 cgcaatgggc gaagcgattg cagatggcat tccgctgatg ggctacacca catggggctg
    24601 tattgattta gtttccgcct ctacgggtga aatgagcaaa cgctacggtt ttgtctacgt
    24661 agaccgtgac gacgcaggca acggcacgct gacgcgcaca cgtaagaaat cgttctggtg
    24721 gtataaaaaa gtgattgcca gtaatggtga agatttagag taagcaacag tgccggatgc
    24781 ggcgtgaacg ccttatccgg cctacatgtc gcacgaacca ggaaggcccg ataagcgcag
    24841 cgcatcgggc aatctggggt ttggcatcag tcaaaatcaa agaaaccatt ccggcttaac
    24901 gccaaatcgg gctgaaagtg ccttaatgtg ggatatcgtc agggatcgtt gtccagacaa
    24961 aatctgactg accagggatt tagagccgat ttcgttcttc aaatccgcat aggtcagatt
    25021 atgttgatca atcagcgtgc gaagtaacgc cacgccaaca ggcattgccg caacggcttt
    25081 gtcgaactct gcaaactttt cgttgttgtt ttcatactct gcaatgcggc tcgccagaaa
    25141 gtcgataagt ggattggtgt catcttcttc aatcaaatat tcgaccagtt ccagtgcttc
    25201 acgataatca tcttctgagt cactgcctcc cagaaagggg actgcattga ccagttctct
    25261 tgttgcttta acagcgcgtg cagcattagc agtcattctt tgttctctct gtagtaacgt
    25321 gtcagtttgt catactctgc gtgattcgtt atgtgcttca caaagaaccg cttattaaca
    25381 aagtttatgt aggcaataac cctaagattg ttcccgccga catccagaac gtaccatttg
    25441 ttgcgatatc taaaattgtc gaggttggga aacgccgttc gcatttcttc tggtgtagaa
    25501 aaatccgttt ccttaataac ccgatacaat gcctgatgag ccagagcgtc gttaggatat
    25561 ttacgcgcag actcttcaaa tggcgcttta gaaatgatgt gcatttttaa gttttaactt
    25621 ccttatagta agtaaacagc cacatctgcg atgttctatc cttatgtgtt cggaaactat
    25681 agctcatcaa atccaagttc acaatatgta aacacttgtg cgcgatataa ttacccccaa
    25741 aatcctcata gccactctgc aaaaagtcag ttctggttca cccctcaagc acatccccct
    25801 ttatcaagca acgctgcgtc tgcgccagat cttccgtttg cgggtcgcgc ccggcgatgg
    25861 gtttgaggac gagtttacgg ttgggggtat aagtaaaacg cgtgagcgcg ggatagctta
    25921 acttgccaaa ccagccgtga acacgctgcc atttggcgtc ttttacctga tacagactgg
    25981 ttatccagta accagaatta aactgcatat ccagcaattg cgtgtgacca ttgccttgca
    26041 aatccagcaa atcatctacg ccagttttgc ttgcggtgta gaatcccctg ggttcaaaga
    26101 cacagggacg gccttcgcgc gtgaacgtca taagaattaa atgtgcataa ggagccagac
    26161 cattaccggt acttggtaac cagataacca gatcctgaat gccgttacga tcaagatcgg
    26221 cgcgatagag ttgccctttg cccgctatgt ttgccagcat acccacaggc acagtccagg
    26281 atttaccggc gcggtcttta gcggttaatt gccagtaatc ttcttcattt ggcatcttct
    26341 caagcgtcac gccagcaacg cccactttcc taaaatgcca ggtttgctcg gcatcataag
    26401 gcatggtcag gagaggtttc agcgcccgct tttgcgcgta ttcgctgtca ctcattggca
    26461 gatattcggt aaagctggtg gcctgtgcag gcagcactaa gctcaataaa aaagcgcaca
    26521 tccctgttct gatgttcaat tccctgaatt ccctgatgga gaattattct tcttccaccg
    26581 ctaactgcgc aaatccacca tttccttccc agccttccag tcgttgataa acggtttcta
    26641 ccgcatcttt aatcggctgg gtcatcgggt agtaaaagcc gacgatatcc ggctgaatgc
    26701 cgaggaaaat cacttcacca atgtcttctt ttaactggtc gataaggtaa ttcaacggca
    26761 tgttatgggt agtcatcata aacatctcgg cgatatcatc cgggtcgatg atgcggattt
    26821 cgccggggtt tagccccata tccgtggcgt cgacaatcag cagtcgtgtc gggcgcagtt
    26881 cacggatggc gacgatgtcg ttttccggtg cactaccgcc gtcaatcacc acccagttac
    26941 ctttcggcgc ggcggcgcac ttttccgcca gcagcgggcc agcgccatca tcgcccatca
    27001 tgctattgcc aacacagagt aaaacgtcag tcacgcagtc tcctcaccat caaatagatg
    27061 gcgttttcct gatgaatatc atgcagcata cttaacagca ttgtactcca ttccttctgc
    27121 tgcgaagttt gcgctgcccg ggctttatcg aatgcgttgg ccagcatcgg cacatggtta
    27181 atgtcgatga cgatctcgcc atatttcggt acgccttcca ttttgcggcg ggcttcgctg
    27241 cccgcctcca gagtggcaat ccatgccaga tattcatccc atgggcaggt gagcgccgct
    27301 tccaggcaat cgataacccc aaggtggtga ccaatcgcca ggctgtaata gaccacctgc
    27361 tgcgcctcgg cgggcgtggc atcgttctca tcaataaatt tacggctcag ttgactgaac
    27421 accacctttt cactcatcgg atacgcgcct cttcaacaac atgattcaga tggctgacaa
    27481 tctcgttcag acgcggatcg ttttccgctt ccagccagcg tgcaacctgt tcttcgccct
    27541 gccctaactg tgtaaggtaa tcatcggcaa tctgacgacc gtaacgataa cccgccaggc
    27601 gacgtgcttc gcgatccact ttcacgcgca gcggctgcac catatcgcca tgcagaatct
    27661 cagccggttg ttcatcctgc tcacccgggc cacgggcgtg aattttctgc tccagcaggc
    27721 cgagtgccat tgcaaagccg tacagcgtgg cggcaggcgt tggcgggcaa cccggaatgt
    27781 agacatctac cgggacgatt ttatccgtac cgccccacac gcagtagaga tcgtggaaaa
    27841 taccaccgct gttaccgcag gcaccgtagg agatacaaat tttcgggtcc ggcgcggact
    27901 gccacgcacg cagcgcaggg gatcgcattg cacgggtgac cgcgccggta aacagtaaaa
    27961 tatccgcatg acgcggtgaa ggaacgactt taatgccgaa gcgttctgca tcaaacagcg
    28021 gcgataaagt ggcgaaaatt tcgatttcgc agccgttgca gccgccgcag tccacgcggt
    28081 aaacataggc agaacgtttg atttttttca gtaacgacgc cttcatgctg gcgatggatt
    28141 catccaccgt catcgggacc ggaatgccgt tggcgtcacg ggggcctaat aaattgctca
    28201 tcagatggcc tctttcatat ggcgagtcag ttcaatacgg tcggacggca ccaggcattt
    28261 ctggcgctta cattccgggc aagtctcaaa gctttcgcgg tggttttccg cgcggctgtc
    28321 accattgtgc ttaagcagcg caatggcgta gtcgatctct ttctggacgg cgaaaggacg
    28381 attgcagacg cgacagctgc acagcacaaa acgggactgt tgcaggaaat cttctttctt
    28441 ccacaccgcc agttcgtact cttgcgacag tttgatcgct gccgtcgggc agacttcttc
    28501 gcagcgtcca cagaagatgc agcgcccgag attaaactcc cacgccagct caccggtggc
    28561 gaggtcagtt tcaaccgtta aggcgtttga cgggcaggca ttgacgcagg ccgcacagcc
    28621 gatgcactgc tgcgggttct gctccggctt accacggaag tttttatcaa ccgcaatcgg
    28681 ctccagcgga taagacgagg tcgccgtgcc ggttttgatg acttttttga taaaggtaaa
    28741 catggcgatt ccttatttca gcggcgagtt tttacgctca atgctgtaac gctcgagttc
    28801 tttgtacggc accactttgc tctttttctt acgcacatcg accacggtca tgcggtcggt
    28861 acaggagtag caagggtcca ggctaccgat aatcagcggc gcatcggaaa cggtgttacc
    28921 gcgcagcatg tagcgcaggg tcggccagtt cgcgtaggtc gcggcacggc agcgccagcg
    28981 gtacagcttc tggttgtcgc cggtcatgct ccagtggata tcgtcgccgc gcggcgcttc
    29041 ggcaaagccc agcgcgaaac ggtgcggaat gtaggtaaag ccttccacca tcagcggacc
    29101 gcccggcagg ttatccagac cgtagtcgat catgttcagc gcggtataaa cttcgttgat
    29161 acgcactttc agacgggaaa taacgtcgca gccctgctcg ctgtggactt ccattggcag
    29221 caggccataa ccgacaaacg ggtgatcggc gcgagtatcg cgggcgtgac cgctggcgcg
    29281 aaccatcggg ccaacgttac tgaaatcgcg ggcgatttcc gggtccagac gaccaatgcc
    29341 gacggtgcgc tgttccatgt tcggcgtact cagcagcaca tccaccagct cctgcacttc
    29401 acgacgcatc tgttgcgcca gctggcgggt ctggatcatg tcatctttca acagatcgcg
    29461 acgaatcccg ccgatcaggt tcaggccgta ggttttacgc gcaccggtga ggatctctgc
    29521 cattttcatg gaggtttcac gcacgcggaa gaactgcata aagccggagt caaagccggt
    29581 aaagtggcag gcgagaccga ggttgagcag atgcgagtgc aggcgttcca cctccagcag
    29641 aatggcgcgg atcatctgcg cacgttctgg cacctgaata cccatcgcgt tttctaccga
    29701 cgtggtgtag gcggtgctgt gggcaaagcc gcagatcccg cacacacggt cagagaggaa
    29761 ggtcacttcg ttgtaaccca tacgggtttc cgccagtttt tccatgccgc gatgaacgta
    29821 gaacagacgg tagtcggcgt cgataatgtt ttcgccatcg acgaacagac ggaagtggcc
    29881 cggttcgtca gaagtgacgt gcagcggacc aatcggcacg acgttgtttt tcttgtcgcc
    29941 cagttcgttg atgaactcgt aggtttcagc atcggtagtc ggtgccggac gctgacgata
    30001 atccatgctg tctttacgca gcggataaag ttcatccggc cagtcatccg gcagcaccag
    30061 acgacgttca tccggcagac caaccggaat caaaccgtac atatcgcgca cttcgcgctc
    30121 gccccacacc gccgccggaa cgcgcggcgt cacggacgga tactccggtt tgttggcgtc
    30181 aacttcgacg cgaaccgtta cccaacactt ggtgcccttc tccatcgaca gcacgtaata
    30241 aacggcgtaa tgaccattca gtttgcgttc gtcgttacca aacagcaccg acagccagcc
    30301 accctgcttg tagtagagaa actccaccac ttccggcagg tagttcacct tcacggtcac
    30361 ggtcagctga tctttggtct gccaggcgtg gtccagcacg acgcccggaa atgcctcatt
    30421 cagcgcggcg agataatgtt gacctaattt ttcttcagac atgctcaaac tctctttaat
    30481 cacgccgcca gcaaggagac gaacgctaaa aatgcaaagc caaacccagc ccaggtaatg
    30541 cgcggagtaa tatcaagacg cagacgggcc atgctgtttt cgaacagcgc gataaccagg
    30601 acgccgacga ccagtttgac gatggcaatc accagcgcca gcagcagccc accggcggtg
    30661 aaggtttcca tctgtcccca cgggataaat acgccgacga acatctgcag caccaccagc
    30721 tgtttcaggc tgatacccca tttcatgacg ccaaagccgc tgccgctgta ttcagagagc
    30781 gggccttcct gcagctcctg ctcggcttcc gccaggtcga acggcagttt gcccatttcg
    30841 ataaaggtgg cgaacgcaca ggcacaaagc gccagtacca gcgggatgct ctggctcagc
    30901 ggccagtgat aaacggtgtc ggtgatgttg ctgatgttgg tggaaccggc aacctgtgcg
    30961 gcaacccaca gaccaagcag cagcatcggt tcgaccagca cgccaagcat cgcttcacgg
    31021 ctcgcgccga tagcggtaaa cgggctaccg gtatccagac cagaaatggc aaagaagaaa
    31081 cgcgcgatgg caaagagata cagtaaggtg attaaatcac ccagttgcgg cagcggagaa
    31141 ccgacggtca ccaccggcag cgcagtagcg atagtcagca tgacgcccac catcacatac
    31201 ggcgtcaggc ggaacaccca gccggaggca tccgggccaa cgctctggcg gcccagcagt
    31261 ttgataatgt cacgatactc ctgcaacaca cccggcccac gacggttatg taagcgggct
    31321 cgcgccacgc gggtaatacc ggagagcagc ggcgcaacgg caaatagcac cagcgcctga
    31381 attaacggat ataaaacact cattctcagg ctcctcgtga aacaataatc accaccagta
    31441 ccgccagttc aaccagcgcc atccggcgga acagcaacgc actcccctcg cactgccagc
    31501 ccggcaccag agacaccgga ttcagccatt tgcgtagttt cagcaccggc gcaaacgcct
    31561 gtttcaccgg catggcaaaa ccgtgagcgg taatcaccat tgatttttcg tggtcgtagc
    31621 cgcacaccca ggccgcgccg cgggaacgcg atggcaaacg atcgcctttg caaatcgcca
    31681 taatgatgaa tggcagcagc ggacaggcaa tcagcagcaa tgtgatcatt ggttgagaaa
    31741 cggtggtgtt agcaggctcc agcggcagag gtacagcagc agacagcatc ggcagcagcc
    31801 acggcgcagc aacaccgcca attacgcagc aaatcgccag tgccactacg cttaccctca
    31861 tcaggagcgg cgcacaggtg gcgttttcgg cttctttggt acgcggcgcg ccgaggaacg
    31921 tgacgccata gactttcgcc atacacatca ccgccagcgc accggtaatt gccagaccca
    31981 cagcgagcag cggccccagc agacgggcaa caaacgcgcc gctattgctc agtttgaaga
    32041 aggattgata gataacccat tccccggcaa acccgttcag cggcggcagc gcagccattg
    32101 ccattaaccc gactaacatg gcgatggaga taaccggcat tttcttgcca ataccaccga
    32161 gtttttcgat atcgcgatga ccggtacgga accagacgct ccccgccccg aggaacagta
    32221 cgcttttgaa caggctatgg ttaagcagat ggtacagacc accgaccagg ccaagagcaa
    32281 tcagcgccgg ttgttcgagc gcgatacccg ttacgccagc gcccagcccc agcaggataa
    32341 tgccgatatt ttccagggtg tggtaagcca gcaggcgctg gatgttgtgc tccatcagcg
    32401 catacagacc accgacaaac gcggtgatca tgccgagcac cagcagcgcg atcccccacc
    32461 acagcggtgc attaccgccc agcagtgaca gggttaaaat gcccagcagg ccaattttca
    32521 tgactaccgt agaaaacaac gcggcagctg gcgcagaggc gttagcatgt gcctgcggca
    32581 cccagccgtg cagcggaata atcccggcga gcaggccaaa gccaatcaca ccgagcagcc
    32641 agatatcgga accgagcggc agctgttgca tacgcatatc cagcaggcgc agatccagcg
    32701 tgccataacg ctgccacagc agccagcaag caatcgccag cagcagagtg ccaagacgcc
    32761 ccagcgcaaa ccacagtttg ccctctttgc tgttgctagt gaggaacacc gcgcacaggg
    32821 ccatgatttc ggccattacc acgaacatgc cgaggttgct ggcaatgacg gcgcagacgg
    32881 cggcagccat caacatattg atctgcaagc cgttgcactt cacctgcgcg tggcgatgcc
    32941 agtcaatgtt atagaggctg acaaacagac cgcaaaggcc gagggtaatc agccaaatcg
    33001 cgttaagcgg ggagatttgc acatcgtagc ttaccagcga cagcgcaccg ctcacgccaa
    33061 ccgcgccagt cagcacagtg aagcccgcgg ctgccgtata caggctacca accgcgccgc
    33121 caattccggc tatccagcca cttagcgtct tttgaaaaga aaagagaaat gccagaacag
    33181 cggcggcgac aaaccacgcc acgccgctat tgatcaggga aattgcgctc atttagcctc
    33241 tccactttga gcctgctgaa acaaggtgag atcgccaaag tccgtgttaa aggtcagctc
    33301 acgcttacgt ttgctgacgc gggcgatatc ggtgttatcc accagatgca gggctttagt
    33361 cgggcacatc cgcacgcagg ccggaccttg ttcatcaaag ctacaaaggt cacatttgac
    33421 ggcgatcgcg cgaatacctg gcacccagtc aagcaatgtg ctgacacgcg ccggagcagg
    33481 cggtgccggt ggcgctttcg gggtattggc gtttgccgga atatccagcg gacggctgcc
    33541 ggaaaattca attgcgccaa acgggcaggc gatgccgcac agcttgcagc ttacgcacag
    33601 gctttcattc aactgcacgg ccccatcaac gcgggtgatg gcgttaaccg ggcagaccac
    33661 cgcgcagggt gcatcttcac agtggtgaca gagctgcggc gcagattctt tttcattcag
    33721 catcactctc aggcgcggca ttgattgcag gccgtgctgg cgatgcgtct ctgaacaggc
    33781 ggcctcacaa gtgtggcagc cgatacagag cgtggagtca gcaattacaa aacgattcac
    33841 caggcattcc tcaggtgatt gtcatttttg acgaaaacat gccgatgaaa tgtcattttc
    33901 gacactcatc gacacgccca tccccaaaca ggcgtaacgc ctgcaaaacg ggcaaagcct
    33961 cagctcatgc tgccgggctt tgtcccttta ccagttggct taaattcacc ggcacattgt
    34021 tttcaatagc ggtgtataag ctgtcgtaaa cgccagcgat tttttcgaga tagtgttcca
    34081 gaacctgttc gcgatcgcgg atgttgacct tgccatcaat catgccgtca atgctcagtt
    34141 gcgcctgcgc aatccgctgt ttgtcttcgc catcttttgt ttcgacgtaa tactcgatgg
    34201 tgtggctgtt aaagccatcc gccagggtga cgtaaatgcg aaaatgttca aaaaggacga
    34261 aacagagttc tttgtccggc gcgcagctca tggcgtgatg caggcgcgct ttcgataacg
    34321 tgatcccctg aaccagcgaa ttgcagtaga tgtgccactg gtcctgtagg cgacgatgcc
    34381 gctgtgcgat gtaatcggct ttctcgctta tttcccaaat agtcattgtc aggttacccg
    34441 tttaacagag atgccagctt taagcatttt ctgtgccaac ttttaattta ttgttattaa
    34501 agagattttt aagctaaaga tgaatttcgt cgccgtgtcg acgtgtcatt tcgacatcat
    34561 cgacattatt caccgcaggg ataatcaaca ctggcacaat tattgcttgt agctggcaat
    34621 agttaatggg aggcgatatg cacgaaataa ccctctgcca acgggcactg gaattgatcg
    34681 aacagcaggc cgcaaaacac ggcgcaaaac gcgtaactgg ggtctggctc aaaattggcg
    34741 cattttcttg tgtcgaaacc agctctcttg ccttttgttt tgatctggtt tgccgcggca
    34801 gcgtggcgga aggttgtaaa ctgcacctcg aagaacaaga ggccgaatgc tggtgtgaaa
    34861 catgccaaca gtatgtgacg ctactgaccc agcgcgtccg ccgctgtcca cagtgtcatg
    34921 gtgacatgct gcagattgtg gcagacgacg gtttacagat tcggcggata gaaatagacc
    34981 aggagtgagc gatgtgtaca acatgcggtt gcggtgaagg caacctgtat atcgagggtg
    35041 atgaacataa ccctcattcc gcgtttcgta gcgcgccatt tgccccggcg gcacgcccga
    35101 agatgaaaat caccggcatt aaagcgcctg aatttacccc cagccagact gaagaaggcg
    35161 acctgcatta cggtcatggc gaagcgggca ctcacgcacc gggcatgagc cagcgtcgga
    35221 tgctggaagt cgaaattgac gtgctggaca aaaataaccg tctggctgaa cgcaaccgcg
    35281 cgcgctttgc tgcccgcaag caactggtgc tcaacctggt ttccagccca ggttccggta
    35341 aaaccaccct gctgacggaa accttaatgc gcctgaaaga cagcgttccg tgcgcggtta
    35401 ttgaaggtga ccagcaaacc gtgaacgatg ccgcacgcat tcgcgctacc ggcacaccag
    35461 cgattcaggt gaacaccggt aaaggctgcc atcttgacgc acagatgatt gccgacgccg
    35521 caccgcgtct gccactggac gataacggta ttctgtttat cgaaaacgtt ggcaacctcg
    35581 tatgcccggc cagcttcgat ctcggtgaaa aacacaaagt ggcggtgctt tccgttaccg
    35641 aaggtgaaga caaaccgctg aaatatccgc atatgtttgc cgccgcctcg ctgatgctgc
    35701 tcaacaaagt tgacctgttg ccgtatctca actttgacgt tgagaagtgc ctcgcctgcg
    35761 cccgcgaagt caatccagaa attgaaatca tccttatttc cgccaccagc ggtgaaggga
    35821 tggaccagtg gctgaactgg ctggagacac agcgatgtgc ataggcgttc ccggccagat
    35881 ccgcaccatt gacggcaacc aggcaaaagt cgacgtctgc ggcattcagc gcgatgtcga
    35941 tttaacgtta gtcggcagct gcgatgaaaa cggtcagccg cgcgtgggcc agtgggtact
    36001 ggtacacgtt ggctttgcca tgagcgtaat taatgaagcc gaagcacgcg acactctcga
    36061 cgccttacaa aacatgtttg acgttgagcc ggatgtcggc gcgctgttgt atggcgagga
    36121 aaaataatgc gttttgttga tgaatatcgc gcgccggaac aggtgatgca gttaattgag
    36181 catctgcgcg aacgtgcttc acatctctct tacaccgccg aacgccctct gcggattatg
    36241 gaagtgtgcg gtggtcatac ccacgctatt tttaaattcg gcctcgacca gttactgcct
    36301 gaaaacgttg agtttatcca cggtccgggt tgcccggtgt gcgtactgcc gatgggtaga
    36361 atcgacacct gcgtggagat tgccagccat ccggaagtca tcttctgtac ctttggcgac
    36421 gccatgcgcg taccagggaa acagggatcg ctgttgcagg caaaagcacg cggtgccgat
    36481 gtgcgcatcg tttactcgcc gatggatgcg ttgaaactgg cgcaggagaa tccaacccgc
    36541 aaagtggtgt tcttcggctt aggttttgaa accaccatgc cgaccaccgc catcactctg
    36601 caacaggcga aagcgcgtga tgtgcagaat ttttacttct tctgccagca cattacgctt
    36661 atcccgacgc tgcgcagttt gctggaacag ccggataacg gtatcgacgc gttcctcgcg
    36721 ccgggccacg ttagtatggt tatcggcact gatgcctata attttatcgc cagcgatttt
    36781 catcgtccgc tggtggtcgc tggtttcgaa ccccttgatc tactgcaagg cgtggtcatg
    36841 ctggtggagc agaaaatagc ggcccacagc aaggtagaga atcagtatcg tcgggtggta
    36901 ccggatgccg gtaacctgct ggcgcaacag gcgattgccg atgtgttctg tgtcaacggc
    36961 gacagcgaat ggcgcggctt aggcgtgatt gaatcttctg gcgtgcacct gacgccggat
    37021 tatcaacgat tcgatgccga agcacatttc cgcccggcac cgcagcaggt ctgcgatgac
    37081 ccgcgcgcgc gttgtggtga ggtattaacg ggcaaatgta agccgcatca atgcccgctg
    37141 tttggtaaca cctgtaatcc tcaaaccgca tttggtgcac tgatggtctc ctccgaagga
    37201 gcgtgcgccg cgtggtatca gtatcgtcag caggagagtg aagcgtgaat aatatccaac
    37261 tcgcccacgg cagcggcggc caggcgatgc agcaattaat caacagcctg tttatggaag
    37321 cctttgccaa cccgtggctg gcagagcagg aagatcaggc acgtcttgat ctggcgcagc
    37381 tggtagcaga aggcgatcgt ctggcgttct ccaccgacag ttacgtcatt gacccgctgt
    37441 tctttcctgg cggtaatatc ggcaagctgg cgatttgcgg cactgcgaat gacgttgcgg
    37501 tcagtggcgc tattccgcgc tatctctcct gtggctttat cctcgaagaa ggattgccga
    37561 tggagacact gaaagccgta gtgaccagca tggcagaaac cgcccgcacg gcaggcattg
    37621 ccatcgttac tggcgatact aaagtggtgc agcgcggcgc ggcagataaa ctgtttatca
    37681 acaccgctgg catgggcgca attccggcga atattcactg gggcgcacag acgctaaccg
    37741 caggcgatgt attactggtt agcggcacgc tcggcgacca cggggcgact atccttaacc
    37801 tgcgtgagca gctggggctg gatggcgaac tggtcagcga ctgcgcggta ctgacgccgc
    37861 ttattcagac gctgcgtgac attcccggcg tgaaagcgct gcgtgatgcc acccgtggtg
    37921 gtgtgaacgc ggtagttcat gagttcgcgg cagcctgcgg ttgtggtatt gaaatttcag
    37981 aagcggcact gccggttaaa cctgccgtgc gcggcgtttg cgaactgctg ggactggacg
    38041 cccttaactt tgccaacgaa ggcaaactgg tgatcgccgt tgaacgcaac gcggcagagc
    38101 aagtgctggc agcgttacat tcccatccac tgggaaaaga cgcggcgctg attggtgaag
    38161 tggtggaacg taaaggtgtt cgtcttgccg gtctgtatgg cgtgaaacga accctcgatt
    38221 taccacacgc cgaaccgctt ccgcgtatat gctaataaaa ttctaaatct cctatagtta
    38281 gtcaatgacc ttttgcaccg ctttgcggtg ctttcctgga agaacaaaat gtcatataca
    38341 ccgatgagtg atctcggaca gcaagggttg ttcgacatca ctcggacact attgcagcag
    38401 cccgatctgg cctcgctgtg tgaggctctt tcgcaactgg taaagcgttc tgcgctcgcc
    38461 gacaacgcgg ctattgtgtt gtggcaagcg cagactcaac gtgcgtctta ttacgcatcg
    38521 cgtgaaaaag acacccccat taaatatgaa gacgaaactg ttctggcaca cggtccggta
    38581 cgccgcattt tgtcgcgccc tgatacgctg cattgcagtt acgaagaatt ttgtgaaacc
    38641 tggccgcagc tggtcgcagg tgggctatac cccaaatttg gtcactattg cctgatgcca
    38701 ctggcggcgg aagggcatat ttttggtggc tgtgaattta ttcgttatga cgatcgtccc
    38761 tggagcgaaa aagagttcaa tcgtctgcaa acatttacgc agatcgtttc tgtcgtcacc
    38821 gaacaaatcc agagccgcgt cgtcaacaat gtcgactatg agttgttatg ccgggagcgc
    38881 gataacttcc gcatcctggt tgccattacc aacgcggtgc tttcccgcct ggatatggac
    38941 gaactggtca gcgaagtcgc caaagaaatc cattactatt tcgacattga cgatatcagc
    39001 attgtcttac gcagccaccg taaaaacaaa ctcaacatct actccactca ctatcttgat
    39061 aaacaacatc ccgcccacga acagagcgaa gtcgatgaag ccggaaccct caccgaacgc
    39121 gtgttcaaaa gtaaagagat gctgctgatc aatctccacg agcgggacga tttagccccc
    39181 tatgaacgca tgttgttcga cacctggggc aaccagattc aaaccttgtg cctgttaccg
    39241 ctgatgtctg gcgacaccat gctgggcgtg ctgaaactgg cgcaatgcga agagaaagtg
    39301 tttaccacta ccaatctgaa tttgctgcgc cagattgccg aacgtgtggc aatcgctgtc
    39361 gataacgccc tcgcctatca ggaaatccat cgtctgaaag aacggctggt tgatgaaaac
    39421 ctcgccctga ccgagcagct caacaatgtt gatagtgaat ttggcgagat tattggccgc
    39481 agcgaagcca tgtacagcgt gcttaaacaa gttgaaatgg tggcgcaaag tgacagtacc
    39541 gtgctgatcc tcggtgaaac tggcacgggt aaagagctga ttgcccgcgc gatccataat
    39601 ctcagtgggc gtaataatcg ccgcatggtc aaaatgaact gcgcggcgat gcctgccgga
    39661 ttgctggaaa gcgatctgtt tggtcatgag cgtggggctt ttaccggtgc cagcgcccag
    39721 cgcattggtc gttttgaact ggcggataaa agctctctgt tcctcgacga agtgggcgat
    39781 atgccactgg agttacagcc gaagttgctg cgtgtattgc aggaacagga gtttgaacgt
    39841 ctcggcagca acaaaatcat tcagacggac gtacgtttaa ttgccgcgac taaccgcgat
    39901 ctgaaaaaaa tggtcgccga ccgagagttc cgtagcgatc tctattaccg cctgaacgta
    39961 ttcccgattc acctgccgcc actacgcgag cgtccggaag atattccgct gctggcgaaa
    40021 gcctttacct tcaaaattgc ccgtcgtctg gggcgcaata tcgacagcat tcctgccgag
    40081 acgctgcgca ccttgagcaa catggagtgg ccgggtaacg tacgcgaact ggaaaacgtc
    40141 attgagcgcg cggtattgct aacacgcggt aacgtgctac agctgtcatt gccagatatt
    40201 gttttaccgg aacctgaaac gccgcctgcc gcaacggttg tcgcccagga gggcgaagat
    40261 gaatatcagt tgattgtgcg cgtgctgaaa gaaaccaacg gcgtggttgc cgggcctaaa
    40321 ggcgctgcgc aacgtctggg gctgaaacgc acgaccctgc tgtcacggat gaagcggctg
    40381 ggaattgata aatcggcatt gatttaactg caaattgccg gacagatctg cctgtccggc
    40441 atactattca cgaggttttt tcggacgata tttttccggc agttctggca ccggacgctt
    40501 gtcatcgatg agatgacgca cggttaagat cggatgacgc cacagcattc tcggcccggc
    40561 ccaacgcata atctgtttca tctcttcacg ctttgcaggc tggtaacagt gcaccggaca
    40621 ctgcttacag gctggtttct cttcgccgaa cacacattta tccagccgct tttgcgcgta
    40681 aacaaacaac gcctcgtaat gctccggctc cgctgacgcc tgcgggcatt tcgcttgata
    40741 aagatcgatc atttttttaa tcgtcagttt ttcacgagag atacgcttgc cggacatgct
    40801 gcctccatac tcttaaggtg tatttatatt acaacttaat ttgaaagggg actatggctc
    40861 tgaaggagga ggtttattca accgacgcaa ttttgcgtac ttgcttcata agcatcacgc
    40921 aaaagctgca aaacagcatc tttcccggaa ccagcatcaa gaactcgcag ttcgcttctt
    40981 cccctgaaat gattaactcc ggtatcatgt gcgccttatg tgattacaac gaaaataaaa
    41041 accatcacac cccatttaat atcagggaac cggacataac cccatgagtg caatagaaaa
    41101 tttcgacgcc catacgccca tgatgcagca gtatctcagg ctgaaagccc agcatcccga
    41161 gatcctgctg ttttaccgga tgggtgattt ttatgaactg ttttatgacg acgcaaaacg
    41221 cgcgtcgcaa ctgctggata tttcactgac caaacgcggt gcttcggcgg gagagccgat
    41281 cccgatggcg gggattccct accatgcggt ggaaaactat ctcgccaaac tggtgaatca
    41341 gggagagtcc gttgccatct gcgaacaaat tggcgatccg gcgaccagca aaggtccggt
    41401 tgagcgcaaa gttgtgcgta tcgttacgcc aggcaccatc agcgatgaag ccctgttaca
    41461 ggagcgtcag gacaacctgc tggcggctat ctggcaggac agcaaaggtt tcggctacgc
    41521 gacgctggat atcagctccg ggcgttttcg cctgagcgaa ccggccgacc gcgaaacgat
    41581 ggcggcagag ctgcaacgca ctaatccggc ggaactgctg tatgcagaag atttcgccga
    41641 gatgtcgctg attgaaggcc gtcgcggcct gcgccgtcgc ccgctgtggg agtttgaaat
    41701 cgacaccgcg cgccagcagt tgaatctgca atttggcacc cgcgatctgg tcggttttgg
    41761 cgtcgagaac gcgccgcgcg gactttgtgc tgccggttgt ctgttgcagt atgcgaaaga
    41821 tacccaacgc acgaccctgc cgcatattcg ttctatcacc atggaacgtg agcaggacag
    41881 catcattatg gatgccgcga cacgtcgtaa cctggaaatt actcagaacc tggcgggcgg
    41941 tgcggaaaat acgctggctt ctgtgctcga ctgcaccgtc acgccgatgg gtagtcgtat
    42001 gctgaaacgc tggctgcata tgccagtgcg cgatacccgc gtgttgcttg agcgccagca
    42061 aactattggc gcattgcagg atttcaccgc cgagttgcag ccggtactgc gtcaggtcgg
    42121 cgacctggaa cgtattctgg cacgtctggc tttacgaact gctcgcccac gcgatctggc
    42181 ccgtatgcgc cacgctttcc agcaactgcc ggagctgcgt gcgcagttag aaactgtcga
    42241 tagtgcaccg gtacaggcgc tacgtgagaa gatgggcgag tttgccgagc tgcgcgatct
    42301 gctggagcga gcaatcatcg acacaccgcc agtgctggta cgcgacggtg gtgttatcgc
    42361 atcgggctat aacgaagagc tggatgagtg gcgcgcgctg gctgacggcg cgaccgatta
    42421 tctggagcgt ctggaagtcc gcgagcgtga acgtaccggc ctggacacgc tgaaagttgg
    42481 ctttaatgcg gtgcacggct actacattca aatcagccgt gggcaaagcc atctggcacc
    42541 catcaactac atgcgtcgcc agacgctgaa aaacgccgag cgctacatca ttccagagct
    42601 aaaagagtac gaagataaag ttctcacctc aaaaggcaaa gcactggcac tggaaaaaca
    42661 gctttatgaa gagctgttcg acctgctgtt gccgcatctg gaagcgttgc aacagagcgc
    42721 gagcgcgctg gcggaactcg acgtgctggt taacctggcg gaacgggcct ataccctgaa
    42781 ctacacctgc ccgaccttca ttgataaacc gggcattcgc attaccgaag gtcgccatcc
    42841 ggtagttgaa caagtactga atgagccatt tatcgctaac ccgctgaatc tgtcgccgca
    42901 gcgccgtatg ttgattatta ccggcccgaa catgggcggt aaaagtacct atatgcgcca
    42961 gaccgcactg attgcgctga tggcctacat cggcagctat gtaccggcac aaaaagtcga
    43021 gattggacct atcgatcgca tctttacccg cgtaggcgcg gcagatgacc tggcttccgg
    43081 gcgctcaacc tttatggtgg agatgactga aaccgccaat attttacata acgccaccga
    43141 atacagtctg gtgttaatgg atgagatcgg gcgtggaacg tccacctacg atggtctgtc
    43201 gctggcgtgg gcgtgcgcag aaaatctggc gaataagatt aaggcattga cgctgtttgc
    43261 cacccactat ttcgagctga cccagctacc ggagaaaatg gaaggcgtcg ccaacgtgca
    43321 tctcgatgcg ctggagcacg gagacaccat tgcctttatg catagcgtgc aggatggcgc
    43381 ggcgagcaaa agctacggcc tggcggttgc agctctggca ggcgtgccaa aagaggtgat
    43441 taagcgcgca cggcaaaaac tgcgtgagct ggaaagcatt tcgccgaacg ccgccgctac
    43501 gcaagtggat ggtacgcaaa tgtctttgct gtcagtgcca gaagaaactt cgcctgcggt
    43561 cgaagcactg gaaaatctcg acccggattc actgactccg cgtcaggcgc tggagtggat
    43621 ttatcgcttg aagagtctgg tgtaataaca attcccgata gtcttttgct atcgggaata
    43681 ttaacgacaa ctgacgaatc aaataaaaat accctgtata ataggaaagc ttattttaca
    43741 gggtaaaacc atgccatcta cacgctatca aaaaatcaat acccatcact atcgccatat
    43801 atgggtcgtt ggtgatattc atggtgaata tcagttatta caatcccgct tacatcaact
    43861 ctcttttttc cccgaaaccg acttacttat ttctgtcggc gataatattg atcgtgggcc
    43921 ggagagtctt gacgtcctgc gcctgctaaa ccagccctgg tttatctccg ttaaaggcaa
    43981 ccacgaagca atggcgctgg atgcgttcgc gaccggcgat ggcaatatgt ggcttgccag
    44041 cggtggtgac tggtttttcg atttaaatga ttcagagcaa aaagaagcta cagatctgtt
    44101 gctgaaattc catcaccttc cacatattat tgaaatcact aacgacaaca taaaatatgt
    44161 catcgcacat gcagattatc cggggagtga atatctcttt ggtaaagaaa tagcggagag
    44221 cgaattactc tggcctgttg atcgtgtgca gaaatcgctt aatggcgagt tacaacaaat
    44281 aaacggcgct gattatttta tatttggaca tatgatgttt gataacattc agacgttcgc
    44341 taaccagatt tatattgata ccggcacgcc gaaaagcggg cggctgtcat tttataaaat
    44401 aaagtagtct catgcttctt ctgtgaagca tgagtaacca aatgttattg caggccatta
    44461 ttcatttttc gctaccagca aagagagatc ctgcttcgcc agcgcgcgac tggcactctc
    44521 cggcaaaccg tcgtctgtaa taatctgatc aaactcgctt aatggtaacg ccagccatgt
    44581 cgccacctga ccatatttcg tcgcatcaca gaccaaaact cgctggcggc tggcactggc
    44641 aatcgcccgt ttcaccgtga ctttatcttc cgctggcgta gaaatccccc gcacactcca
    44701 tgacgatgca gaaataaaag cctgatcaat catcaggctg cgcagcatgg tcgcagcggc
    44761 ttccccgaca caggaacggt tttcccgaca cactgcaccg ccagtgtgaa taattgtgca
    44821 attactgttg tcgagcaagt agttcgcaat aacgaaatcg tttgtgacca cagtcagtga
    44881 ctccatgtga atcagatgct gtgctatcgc taacgtggtc gttcccgcat ccagatagat
    44941 acaacttccc ggctgaacaa gacttgccgc cagcttgcca atagccgctt tttgcgtcat
    45001 tgccagcgca gtttttacct gatgagaagg ttcatgcgcc acgcgtcccg gagactggac
    45061 gcctccggac accagcacaa cggctccctg ctgctccagt ttttgtaaat cccgacgaat
    45121 ggtcatatgt gacacattca ttctgtccgt tagttcagca atactgacaa tgcctttttc
    45181 agctaccatc tcaaggatga tttggcgacg ctctacgggt atcaactttt gctccttcct
    45241 ttgtcctgct gacattctac gctatttgcc tgcgaaacgt gcgcggcgca actaacgctt
    45301 agttcacata aaataacaca caatgttaat ttatgtgaat cagctcacca tatcgttatc
    45361 ttccagcgct tatattcaca atatcaaaca aaatatcact taaattaaca aggagagcag
    45421 atgaaaacgg gatctgagtt tcatgtcggt atcgttggct tagggtcaat gggaatggga
    45481 gcagcactgt catgtgtccg cgcaggtctt tctacctggg gcgcagacct gaacagcaat
    45541 gcctgcgcta cgttgaaaga ggcaggtgct tgcggggttt ctgataacgc cgcgacgttt
    45601 gccgaaaaac tggacgctct gctggtgctg gtggtcaatg cggcccaggt taaacaggtg
    45661 ctgtttggtg aaacaggcgt tgcacaacat ctgaaacccg gtacggcagt aatggtttct
    45721 tccactatcg ctagtgctga tgcgcaagaa attgctaccg ctctggctgg attcgatctg
    45781 gaaatgctgg atgcgccagt ttctggtggt gcagtaaaag ccgctaacgg tgaaatgact
    45841 gtcatggcct ccggtagcga tattgccttt gaacgactgg cacccgtgct ggaagccgtt
    45901 gccggaaaag tttatcgcat aggtgcagaa ccgggactag gttcgaccgt aaaaattatt
    45961 caccagttgt tagcgggcgt acatattgct gccggagccg aagcgatggc acttgcagcc
    46021 cgtgcgggga tcccgctgga tgtgatgtat gacgtcgtga ccaatgccgc cggaaattcc
    46081 tggatgttcg aaaaccggat gcgtcatgtg gtggatggcg attacacccc gcattcagcc
    46141 gtcgatattt ttgttaagga tcttggtctg gttgccgata cagccaaagc cctgcacttc
    46201 ccgctgccat tggcctcaac agcattgaat atgttcacca gcgccagtaa cgcaggttac
    46261 gggaaagaag acgatagcgc agttatcaag attttctctg gcatcactct accgggagcg
    46321 aaatcatgat caagattggc gttatcgccg atgattttac cggcgcgacg gatatcgcca
    46381 gttttctggt ggaaaacggt ctaccaacgg tacaaattaa cggtgttcca acaggtaaaa
    46441 tgccggaagc aatcgacgca ctggtgatca gcctgaaaac gcgctcctgt ccggtggttg
    46501 aagccacaca gcaatcgctg gcggctctga gctggttgca acagcaaggt tgcaaacaga
    46561 tctatttcaa atactgctct actttcgaca gtacggcgaa aggtaatatc ggcccggtta
    46621 ccgatgcatt aatggatgct ctcgacacgc cgtttacggt cttctctccg gccctgccgg
    46681 tcaacggacg tacggtttat caggggtatt tgttcgtaat gaatcaactg ctggccgaat
    46741 ccgggatgcg ccatcatccg gtaaatccca tgaccgacag ctatcttccc cgtctggttg
    46801 aagcgcaatc cacagggcgc tgcggcgtcg tttcggcaca tgttttcgaa caaggtgtgg
    46861 atgccgttcg tcaagagctg gctcgcttac agcaagaggg ctaccgctac gcggtgcttg
    46921 atgcgctgac cgaacaccat ctggaaattc agggagaagc cttgcgcgat gccccactgg
    46981 taacgggcgg ttctggtctg gcgattggcc tggcccggca gtgggcgcaa gaaaacggta
    47041 accaggctcg cgaagcaggg catccgctcg ctgggcgcgg cgtagtgctc tccggttcat
    47101 gctctcaaat gaccaaccgc caggtggcac attaccgtca aattgcacca gcccgtgaag
    47161 ttgatgtggc acgctgcctc tcaaccgaaa ctctggccgc ttatgcacac gaactggcag
    47221 agtgggttct gggccaggaa agtgtacttg ctccactggt ttttgccacc gccagcactg
    47281 acgcattggc agcaattcaa cagcaatacg gtgcacaaaa agccagtcag gcagtagaaa
    47341 ctctattttc tcaactagcg gcgcggttag cagcggaagg cgtgacacgc tttattgtcg
    47401 caggcggtga gacctccggc gtagtcacac agagcctggg cataaaaggg tttcatattg
    47461 gcccaaccat ttcccccggc gtgccgtggg taaacgcact ggataagcct gtctcactcg
    47521 cccttaaatc tggcaacttc ggtgatgaag cctttttttc acgagcccaa agagagtttt
    47581 tatcatgagc gatttcgcaa aagtagagca gtctttgcga gaggagatga cgcggattgc
    47641 cagttcattc tttcagcgcg gctatgcaac cggttcggct ggcaatctgt cgctgctttt
    47701 acctgacggg aatttactgg cgacaccgac aggttcatgc ctgggcaatc tcgatccgca
    47761 gcggctttcc aaagtcgccg cggatggcga atggttaagt ggtgacaaac cctcgaaaga
    47821 ggtgctcttt catctggcgc tgtatcgcaa caatccgcgc tgtaaagcgg tggtgcattt
    47881 gcacagcaca tggtcgacgg cgctttcctg cctgcaaggg ctggacagca gcaacgttat
    47941 tcgtccgttc acaccatacg tggtgatgcg gatgggaaat gtcccgctgg tgccttatta
    48001 ccgaccgggc gataaacgca tcgcacagga tctggcggaa ctggcagcag acaatcaggc
    48061 ttttttactg gcaaatcatg gcccagtggt ttgcggtgaa agcctgcaag aagccgccaa
    48121 caatatggaa gagctggagg aaacggcaaa gctgattttt attctcggtg accgcccgat
    48181 ccgttatctg accgcaggtg aaattgcgga attaaggagt taatgcaatg cctcgttttg
    48241 cagctaattt atccatgatg ttcaccgaag tgccttttat tgaacgcttc gccgcagcgc
    48301 gaaaagccgg gttcgatgct gtggaatttc tgtttcccta tgactactcc accctgcaaa
    48361 tccaaaagca actggagcaa aaccatctga cactggcgct gttcaatacc gctcccggag
    48421 atattaatgc gggggaatgg ggattatccg cccttcccgg acgtgagcac gaagcacacg
    48481 cagacatcga cctggcactg gaatatgcat tggcgctcaa ttgtgaacaa gtccatgtga
    48541 tggcaggcgt cgtgcccgct ggcgaagacg ctgaacggta ccgggcagta tttatcgata
    48601 atctccgcta cgctgccgac cgctttgcgc tacacggcaa gcgaatatta gttgaagcac
    48661 tcagccccga cgttaagcct cattatctct tctccagcca gtatcaggca ctggctattg
    48721 ttgaagaggt tgcgcgagat aacgtgttta ttcaactaga tacttttcat gcgcaaaaag
    48781 tagatggcaa cctgacgcat ttaatccgcg actacgctgg gaaatatgcg cacgtacaaa
    48841 ttgccggact acctgatcga catgaaccgg acgatggaga aatcaactat ccgtggctgt
    48901 tccgcctgtt cgatgaggtg ggatatcagg gctggatcgg ttgtgaatat aaacctcgtg
    48961 gcctcaccga agaagggctt ggctggtttg acgcctggcg ctaattcgtt aaatccctaa
    49021 ttacaacgta cccatacatc cccccatcta cggatggggt aggttaacta taactttcag
    49081 acagggtttc ccatgtccac aattacattg ttatgcattg cgttagctgg cgtaatcatg
    49141 ctgttgctgc tggtcatcaa ggcaaaggta caaccattcg ttgctctgct cctcgtcagc
    49201 ctgttagtcg cacttgcggc gggtataccg gcgggcgaag tgggtaaagt gatgatcgcc
    49261 gggatgggcg gtgtgcttgg ttccgtcact attattattg gtctgggcgc tatgctgggg
    49321 cgtatgatcg aacactctgg tggtgcagag tcactggcta attatttcag tcgcaagtta
    49381 ggtgacaaac gaactatcgc tgcgctgact ctggcagcgt tcttcctcgg tattcccgtc
    49441 ttctttgatg tcggctttat tattcttgcg ccaatcattt acggttttgc caaggttgcc
    49501 aaaatatcgc cactcaaatt tggcctgcct gtcgctggaa tcatgctaac tgttcatgtg
    49561 gcggtaccgc cgcatccagg ccctgtcgcc gcagcggggt tactccacgc agacatcggc
    49621 tggctaacca tcatcggtat tgcgatttct attcccgtag ggattgttgg ctactttgca
    49681 gcgaaaataa tcaatacgcg ccaatatgcg atgtcggtgg aagtactgga acagatgcaa
    49741 ctggctccgg ccagtgagga aggcgcaaca aaattaagcg ataaaataaa tccaccgggc
    49801 gtcgcgctgg tcacctcgct aattgttatt cctatcgcga ttatcatggc gggtacggtt
    49861 tccgcaacac tgatgccgcc ttcgcatccc ctgcttggta cgctacagct gatcggctca
    49921 ccaatggtag ctctaatgat tgcgctggtg ctggcattct ggttattggc tttacgtcgc
    49981 ggctggagct tacaacacac cagcgacatt atgggctcag cccttcccac tgcggcggta
    50041 gtgattttgg ttactggtgc tggaggggta tttggcaaag tgctggtgga atcgggcgtc
    50101 ggcaaagccc ttgccaatat gctacaaatg attgacctgc ctctgttacc agccgcattt
    50161 attatttcac tggctctgcg tgcatcgcag gggtcagcaa ccgtagcaat cctgacaacc
    50221 ggcgggttac tctcagaagc ggtgatggga ttgaatccga ttcagtgcgt attggtgacg
    50281 ctggcagcct gctttggtgg gcttggcgcg tcacatatta atgactcagg gttctggatt
    50341 gtgaccaaat atctggggtt gtcggtagca gacggtctga aaacctggac agtgttaacg
    50401 accattctcg gttttaccgg attcttaatt acctggtgcg tatgggcggt aatttgacct
    50461 taataaaaag gtccgatggg catcggacct tttatttaac gcttaccttc caccagcagc
    50521 ggcgggatgc tcggcaccat tggcgcgtca tcaatatctt tctgcgtcat gcggaacgct
    50581 tcgggataat gttcgcggct ggtacggcgc agcgattcgg tatcgcgcca ggtataaagg
    50641 caatgctggc actgatatac cgtccagaca tctttcaccg gcgatttcgc catcacttca
    50701 atctgttcat cggcacaacg tggacaaatc atcttcttct ccttatttac gtgcagccag
    50761 catagcggtc agtttttcag cccaggcttt ggtttccggt aaatccacca ccggttggct
    50821 gtagtgacca cggttgtccg gggcgacagg cgtagtggcg tcgataatca gcttgtcggt
    50881 gatccccgca gggcttgagc ctggatcgag ttccagcacg gacatattcg gcaactgcac
    50941 caaatcccct gccgggttca ctttcgagga gagcgcccac atcacctgcg gcaggttgaa
    51001 cgggtcaacg tcttcatcga ccataatcac catcttcacg tagcccagac catgcggcgt
    51061 ggtcattgcg cgcaggccca ccgcgcgggc aaagccgccg tagcgttttt tggtggagat
    51121 aatcgccagc aggccatggg tgtacatggc gtttaccgcc tgcacttcag ggaactcggc
    51181 tttcagctgc tgatacagcg gcacgcaggt ggctggcccc atcaggtagt cgatttcggt
    51241 ccacggcata ccgaggtaca gcgattcgaa aatcggcctg gtgcggtaag agactttatc
    51301 gatgcgcacc acggtcatgt tacgcccgcc ggagtagtgc ccggtaaact caccgaacgg
    51361 cccttcgatt tcgcgtttac ggctttcgat gaccccttcg aggatcactt ctgaacccca
    51421 cggcacatca aaaccggtca acggggcggt ggcgatcggg tacgggcttt cacgcagcgc
    51481 gcctgccatt tcgtactcgg actgatcata tttcagcggc gtggccccca tcagcgtgat
    51541 gatcggatcg ttaccgagcg tgatcgcaat cggcagatct tcaccgcgct cttctgcttt
    51601 atgcagatgc agggcgatat cgtgcatcgg caccggttgc aggccgagct tacgcttgcc
    51661 cttcacttcc atgcggtaga tgccgacgtt ctgcttgccg aagttatccg ggtcgagcgg
    51721 atcgcgggaa accacgcacg ctttgtcgag atagaaaccg ccatcgccat cgtttaaacg
    51781 aaacagcggc aggatatcga acaggttgat ctcgtcgcca tcaacggtgt tctgcgccca
    51841 ggctggattg gcgcggcgct ccggggcaat cgggaagtta tcccagcggc ggataaactc
    51901 atcaatctgc tttttaaccg gggcatttgg cggcaggccg agggaaatcg cgtggttctg
    51961 ccaggaaccg atggtgttca tcgccacgcg ggcatcggta aagccgcgaa tattatcaaa
    52021 ccacagcgcg ggcgcgccgt cgccgatacg cccggtggcg ttagctgctg ctgccagatc
    52081 cggctcggcg ttcacttctt cgctgatttt cagtaactgg ccgtggtcat caagcgcctg
    52141 taaaaagctg cgtaaatcat caaatgccat tattcattct cctgagaaaa attccgggcc
    52201 tgcggcaatc cttgccagcg cctggcgtgg ggatgttcga ggccaaattg atccagcacg
    52261 cgggctacca catggtggac aatgtcatct accgtttcgg gatggttata aaaggcaggc
    52321 atcggcggca ccatcgccac gcccatgcgt gaaagtgcga gcatattttc gagatggatg
    52381 gtgctaagcg gcatttcacg cggcaccagc accagtttgc ggccttcttt gagcacgacg
    52441 tccgccgcgc gccctaccag gccatcggcg taaccagcgc ggataccggc gagcgttttc
    52501 atactgcacg gaataacgat catgccgtcg gtacgaaaag aaccggatga gatgatcgcc
    52561 gcctgatccg ccgggttatg gctgaagtct gcgagggcag caacatcgcg agcgctgtaa
    52621 ggcgtttcca gttcaatggt ggttttcgcc cacttcgaca tcaccagatg agtctcgaca
    52681 ttcggcatct cccgcagcgc ttgcagtaat gccacaccaa gaggcgcacc ggtagcccct
    52741 gtcatcccga cgatcagttt cattgcttat cctcttaatt gttcgtatac gaacgttatt
    52801 attaacatac tccttcattt ccacagcggc aaggattgaa aagtaacaac atcttcttcg
    52861 tcactaaaaa accgctatga tagcgacaga gaattagccc ggagtttaca tggcgttacg
    52921 aaataaagcg ttccatcagt tacgacagct ttttcagcag cacacggctc gctggcagca
    52981 cgagttacct gacctgacca aaccacagta cgcggtgatg cgcgccattg ctgataaacc
    53041 tggtatcgaa caggtggcac tgatagaagc agctgtcagc accaaagcaa cgttagcaga
    53101 aatgttggca agaatggaaa atcgtggctt agtcagacgt gaacatgacc ccgctgataa
    53161 gcggcgacgc tttgtctggt tgactgccga aggcgaaaag atacttgctg cggcgatacc
    53221 cattggtgac agcgtggatg aggaattttt ggggcgtttg agtggagaag agcaggagct
    53281 gtttatgcag ctggtgcgca agatgatgaa cgcatagggg gcaaatttcc ggatgcgacg
    53341 ctatcgcgtc ttatccagac tacaaataca ccgactcacc aaccgaaaga aaaaaggcca
    53401 gcctcgcttg agactggcct ttctgacaga tgcttactta ctcgcggaac agcgcttcga
    53461 tattcagccc ctgcgtttgc aggatttcgc gcaaacggcg caggccttca acctgaatct
    53521 ggcgaacacg ttcacgggtg aggccaattt cacgacctac atcttccagt gttgccgctt
    53581 cgtaccccag caaaccgaat cgacgtgcca gcacttcacg ctgtttggcg ttcagctcga
    53641 acagccattt gacgatgctc tgcttcatat cgtcatcttg cgtggtatct tccggaccgt
    53701 tctctttttc atcggccagg atgtccagca acgctttttc ggaatcacca cccagcgggg
    53761 tgtctaccga ggtaatgcgc tcgttaagac gaagcatacg gctgacgtca tcaactggct
    53821 tatccagttg ctctgcgatc tcttccgcac ttggttcatg gtccagctta tgggacaact
    53881 cacgtgcggt tcgcaggtaa acgttcagct cctttacgat gtgaatcggc aaacgaatag
    53941 tacgggtttg gttcataatc gcccgttcaa tcgtctggcg aatccaccag gttgcgtatg
    54001 ttgagaagcg gaaaccacgt tccgggtcaa acttctctac cgcgcggatc agccccaggt
    54061 tgccctcttc gataaggtcc agcaacgcca gaccacgatt gccataacgg cgggcaattt
    54121 ttaccaccag acgcaagtta ctctcgatca tccggcggcg agaggcgaca tctccacgca
    54181 gtgcgcgacg cgcaaaataa acttcttctt cggccgttaa cagtggtgaa taaccaatct
    54241 caccaaggta aagctgagtc gcgtccaaca cacgctgtgt ggctccctgc gataacagtt
    54301 cctcttcggc caaatcgtta tcactgggtt cctcttctac taaggccttt tcgtcaaaaa
    54361 cctcaactcc gttctcatca aattccgcat cttcatttaa atcatgaact ttcagcgtat
    54421 tctgactcat aaggtggctc ctacccgtga tcccttgacg gaacattcaa gcaaaagcct
    54481 ggttccgccg atttatcgct gcggcaaata acgcagcggg tttacggatt tccccttgta
    54541 acgaatttca aaatgcaagc gtgttgaact ggttccggtg ctacccatgg ttgctatttt
    54601 ttgccccgcc ttcacttctt gttgttcccg gaccagcatt gtgtcgttat gggcgtaggc
    54661 actcaggtaa tcatcattat gtttgatgat aatcagatta ccgtagccgc gcagcgcgtt
    54721 accggcataa acaacgcggc catctgcggt cgcgataatt gcctgtcctt tgctgcctgc
    54781 gatatcaatc cccttgttgc ccccctcaga agcgccaaag gtttcgatca ctttgccctc
    54841 agtcggccag cgccaggtgg agataggcgt actggttgat gtactgctga cagttggctc
    54901 ggttgtgctt gctgttggta ccgttacagg cgctgtgacc gtggtcgcag ctggcttgtt
    54961 gttcggcaac attttgttag cactctgttc acccgaagat tcagaatacg taattgtcgg
    55021 ttgcgacgca acagcaacgg tggaattttg tgcaggcttg atcacaactc cttgctctgc
    55081 tgcgtcggcc tgggtaatgg catttccgcc agtgattggc gtaccggaag cattacccac
    55141 ctgcaaggtc tgaccaacgt tcagcgcgta tggtgcctga atattgttgc gctgagcaag
    55201 gtcacggaaa tcgttgccag taatccaggc gatatagaaa agtgtgtcgc cttttttcac
    55261 ggtataggta ctgccgctat aactgccttt cggaatgttc ccatactgac ggttatagac
    55321 gatgcgtccg ttttccatct gtaccggctg ctgagctact ggctgcactg gctggatttg
    55381 cggttgttga gtagcctgaa tttgtggctg ctgtaccggc tgaatttgcg gttgctgggc
    55441 tgtagacgtc gtccccattt tcggcggcgg cgtaatcaac ataccagaat tggtatttgc
    55501 aggcgcattg ccattaacgg agctgaccgg tgccggtgga tttgaagtgt cagaacagcc
    55561 tgccagccat agcgaaacca gtgacaaagc cgcaatgcgg cgaacggtga attttgggct
    55621 tcccgcgctc atttatcccc caggaaaaat tggttaataa ccagtgacat aattaccgtg
    55681 caaggcaccc tactgaacac tggaaaagat gttcacgata cgctgacctg cggcaaaata
    55741 accaggaaaa atccaggtat ttcctcacgt tttaagccag ctcacccttc actaaaggta
    55801 caaagcgcac ggcctccacg gtatcgataa taaattcgcc tccccgacga cgcacccgtt
    55861 tcaaatactg gtgctcctcc cctacgggta agacgagaat cccgccttcg tccagctgcg
    55921 tcattagcgc agttggaatt tccggcggtg ccgccgtaac aatgatagcg tcaaacggcg
    55981 cgcgtgcctg ccaaccttgc catccatcgc catgacgggt tgaaacatta tgtaaatcaa
    56041 gatttttcag gcggcgacgc gcctgccact gcaaaccttt aatccgttcg accgagcaaa
    56101 catgctggac aagatgcgcc aggattgccg tttgatatcc cgaaccagtg ccaatttcca
    56161 gcacccgcga ctgcggcgtc agctcgagta attcggtcat tcgcgccacc atatacggct
    56221 gcgaaattgt ctgcccctga cctatcggca aggcgatatt gtcccaggct ttttgttcaa
    56281 acgcttcatc aacgaatttt tcacgcggca cggcggcaag tgcattcagc acctgctcat
    56341 cctgaatacc ttgcgcacgt aattgatcca gaagtgcttg tacgcgtctg cttaccattg
    56401 cgtgccaact cccacgctgt ttaaccagtc tgaaaccaca tcttgcgcgc tatgcgcagt
    56461 taaatccaca tgcagcggcg tgatggagac atagccctca tctaccgcag caaaatcggt
    56521 ccccggaccg gcatcacatt taccgcccgg cgggccaatc cagtacagcg tattgccgcg
    56581 cggatcttgc tgcgggatca cctgatctgc cggatgtcgt gtaccgcagc gcgtcacgcg
    56641 aatacctttg atttgatcca agggtaaatc cggaacgtta atattaagaa tacgcccggt
    56701 gcgcaacggc tctttacaca gtgcgcgcaa aattgaacag gttaccgccg cggcagtgtc
    56761 gtaatgttta tgcccgtcaa gcgagacggc aagcgccgga aaacctaaat gacggccttc
    56821 catcgcggcg gctaccgtac cggaataaat aacatcatcc cccagattcg gcccggcgtt
    56881 aattccggac acaacaatgt ccgggcgcgg acgcatcaga gcattcacgc caagatagac
    56941 gcaatcggtc ggggttccca tttgcacagc aatatcacca ttttcaaagg taaacgtgcg
    57001 cagggaggat tccagtgtca gagaatttga agcgccgctg cggttacgat cgggggcgac
    57061 cacctgaacg tcagcaaact cacgcaaggc tttcgccagc gtttgtatac cgggtgcatg
    57121 taccccgtca tcattactca gcaatatgcg cataatcacc tgttgtgttg ataagttccc
    57181 tgacaacgct ggttgcaaaa ctacccgccg gaagccagaa acggatctct acggtgacgt
    57241 catcccacca attccagctt aattgttgcg gatacagcag catcgctctg cgcgcggctt
    57301 caactttttc gcgcaccagt aaagcttgta attcagtttc tgcggcgaca gctgcttgtt
    57361 cgaatgccag cgcttcacgc tgagttcccc attcgccact gcccggcaat gcggcggtta
    57421 tcatcagctc tttatcgtta acgcgacgct gtaattccgc cagttcttcg gtggttgcga
    57481 caaaccagct accacgtccg gctaattgta gcgcatcgcc gtcaacaact tgattaacgt
    57541 ctgctttttt gaggcgctca gcaacaatct gattaaacaa cgcactgcgg gctgccgaca
    57601 accaaaaact ccgtttattg cgatcgcgca ccggagtatt ggtttgcgcc cagcgctgcg
    57661 ccccctgcaa gttgctaccg ccaatcccaa aacgttgggc accgaagtag ttcggtacac
    57721 cttttacgca aatatcgttc agacgttgtt caacgtcatc gcgattgctc acttcgcgca
    57781 gaaccagggt aaaggcgttt cctttcagcg cccctaaacg cagcttgcgc ttgtgccgcg
    57841 catactccag cacctggcag ccttccagtt gaaaggcgct cagatcgggc atttccttgc
    57901 ccggcacgcg agcgcataac cactgttccg taacagcatg tttgtctttt tgcccagcga
    57961 agctgacttc acgggcatga attttcagga atttcgccag tgcatccgcc acaaaacggg
    58021 tattgcagcc gtttttgagg attctaacca gaatatgctc accttcacca tcaggctcaa
    58081 agcccaaatc ttccaccacc acaaagtctt ccggattggc tttcagcagc ccggtgcctt
    58141 gcggtttacc gtggaggtaa gtgagattat caaactcaat cattttgttg ccttaatgag
    58201 tagcgccacc gcttcacagg caatcccttc cccacgtccg gtaaatccca gtttttccgt
    58261 agtagtggct ttcacgttaa catcatccat atggcagccg agatcttcgg caataaacac
    58321 gcgcatttgt ggaatgtgcg gcaacatctt cggtgcctga gcgatgatag tgacatcgac
    58381 gttgccaagg gtataaccct tcgcctgaat acgacgccag gcttcgcgta gcagctcgcg
    58441 gctatcggca cctttaaatg ccggatcggt atccgggaac agcttgccga tatcccccag
    58501 cgccgccgcg ccaagcaatg catcggtcaa cgcatggagc gccacgtcgc catcagaatg
    58561 cgccagcaat cctttttcgt aaggaatgcg tacgccacca atgataattg ggccttcacc
    58621 gccaaaggca tgtacgtcaa aaccgtgtcc aattcgcatt atgtattctc ctgatggatg
    58681 gttcgggtga ggtaaaactc ggccagtgcc aaatcttccg ggcgcgtgac tttaatgtta
    58741 tccgcacggc cttcgaccaa ctgaggatgg aatccgcaat attccagcgc cgaggcttcg
    58801 tcggtaatag tcgcgccttc atttagagcg cgcgtcagac agtcatgtaa cagctcacga
    58861 gggaaaaatt gcggcgtcag cgcgtgccat aagccgttgc gatcaacggt atgagcaatg
    58921 gcatttttgc ccggttcggc acgtttcata gtatcgcgca ctggtgcggc gaggatcccc
    58981 cccgtgcggc tggtttcgct caacgccaac aatcgcgcga ggtcatcctg atgcaaacaa
    59041 ggacgagcgg cgtcatgcac caatacccac tgcgcgtcgc cagcggcttt cagacctgcc
    59101 agcacggaat cggcacgctc atcaccgcca tctacaacgg tgatttgcgg atgattcgcc
    59161 agaggaagtt gtgcaaaacg gctatcgcca ggacttatgg caatgacgac tcgtttcacc
    59221 cggggatgcg ccagcagcgc atgcaccgag tgttcaagaa tggtttgatt accgattgag
    59281 agatattgct taggacattc cgtttgcatt cgacggccaa atccggccgc cggaaccacg
    59341 gcgcaaacat ccaaatgagt ggttgccatg ttaattcccg ggctgattta tcgattgttt
    59401 tgccccgcag actgtgcgcg cttcgacgcg tcaggcacca gacgataaaa agtttcgccc
    59461 ggcctggtca tgctgagttc attacgcgca cgctcttcga gcgcctcctg gccgccattg
    59521 agatcgtcaa tttcggcaaa aagttgatcg tttcgcgctt taagtttcgc gtttgtagct
    59581 tgctgtgccg ccacatcatc attgacgcgg gtatagtcat gtataccgtt cttaccgaac
    59641 cacagcgaat actgtagcca gaccagaata gccagcaaca gcagcgttag tttacccatc
    59701 ctgccccctg aaaaacggca tcatcatccc atgcatccga agacgactct acatcctctg
    59761 ttggggatac cgcgacaacg cgggcaaatg taccacattt gtccattgtt acgtataccc
    59821 agggcgtgca gaacataatc tcattattag ttacggtttg aattatgaac agaggagacg
    59881 ggaaagtaca aattagccca gtagccacat aaacagtgcg ccaaacataa tgcctactgt
    59941 caccagggtg aaaacaatac tgtagcgtag ctttccgtcc atcaatgaat gcagcgcaat
    60001 ccccaccact accgcgacgg gcatcagcgc cagaaagaaa ggccaggtgt agataaagaa
    60061 gaacagcgtg ttagagccat aaatcaacat cggcatcgcc agcgcaaata accaggagat
    60121 aaaaccgacc acggcaccag gcagtgacca tgtggtttct tcatcctcag taaggctgtc
    60181 gttatttgtt agtgtaatgt tatggctatt acgcatattt gatcctgtta ctttgacgaa
    60241 ccgggcatgg aaacccggtg gtgtctcagg atctgataat atcgttctgt ctcaacagat
    60301 ctaataattg ctgtaccaaa tttgttacta attgttcacc attgagatga atttctgccg
    60361 attcaggcgc ttcgtaaacg gaatctattc ccgtaaagtt gcgcagttca ccggcacgcg
    60421 ctttcttata taagcctttt ggatcgcggg cttcgcaaat cgccagcggc gtatcgacaa
    60481 acacttcgat aaagcgccct tctcctacgc gttcgcgaac catctggcgt tcggcgcggt
    60541 gtggcgagat aaatgcggtc agcactacca gtccggcttc aaccatcaaa ttcgccactt
    60601 caccgacgcg acggatattc tctttacgat cggcatcgct aaaaccgaga tcgctgcata
    60661 atccgtggcg aacattatcg ccatccagca gatacgtact gacgccgagt ttatgtaacg
    60721 cctcctccag cgccccggcg accgttgatt taccggaccc ggagaggccg gtaaaccaca
    60781 gcactacacc acgatgaccg tggtgtagct cgcgttgttg cacagtgacc ggatggctat
    60841 gccagacgac gttttcgtca tgcagcgcca ttatttatcc cccagcaaat cgcgcgcgcc
    60901 ccagtgcgga aagtggcgac gaaccagagc attcaattcc agttcgaatg cactgaattc
    60961 agatggcgca gcagttgcct ggctaactgg ctcgtgcacc ataccggcac ccacggtcac
    61021 attgctcagg cgatcgataa aaatcagccc acccgtcacc ggattttgtt gataacgatc
    61081 taacaccagc ggctcgtcaa aagtgagatc cacgaggccg atcccattca gtggcaggtt
    61141 ttcaacttca cgctgggtaa ggttattaat atcaacctga tagcgaatgc catcaacacg
    61201 cgcgcgcgtc ttcttaccgg caattttgat gtcgtaactc tgccctggag aaagcggctg
    61261 ttccgccatc cataccacat ccaccgacgc gctctgcacc gccggtaacg cttcgtctgc
    61321 cgccagcagc agatcgccac ggctgatgtc gatctcatcc gtcagcacca gggtgatcgc
    61381 ttctccggca aaggcttctt cgcgatcacc atcaaaagtc acgatccgcg cgacgtttga
    61441 ttccacacca gagggcagca ctttgacacg ttgcccgact tccacgcgac cggatgccag
    61501 cgttccggcg taaccacgaa aatcgagatt cgggcgatta acgtactgca ccgggaagcg
    61561 cattggctga gcatccacca ctcgctggat ctccacggtt tccagcactt cgagcagtgt
    61621 cggaccgctg taccacggca tactttcact ttgcgatgcc acgttgtcgc cttccagtgc
    61681 agagagcggc acaaagcgga tatccagatt acccggcagc tgcccggcaa aggtcaaata
    61741 atcttcacga atacgggtga acgtctcttc actgtaatcc accagatcca ttttgttgat
    61801 cgccacgacc agatgtttgc tccccaacag tgtggagata aaactgtgac gacgggtttg
    61861 atcgagcacg cctttacggg catcgatcag taagatcgcc agttcacatg tcgatgcgcc
    61921 agtcgccata ttgcgggtgt actgctcgtg ccctggggtg tcggcgataa taaatttacg
    61981 cttctcggta gagaaatagc ggtaggccac gtcaatggtg atgccctgtt cgcgctcagc
    62041 ttgcaggccg tccaccagca gagccagatc cagcttttcg ccctgggtgc cgtgacgctt
    62101 actgtcgtta tgcagcgatg agagctgatc ttcgtagatt tggcgggtat cgtgcagcag
    62161 acgaccaatc agagtacttt tgccgtcatc gacgctacca caggtcagaa aacgcagcag
    62221 gcttttatgt tgttgcgcaa tcatccaggc ttcgacgccg ccttcattgg cgatttgttg
    62281 tgcaagtgcg gtgttcatct taaaaatacc cctgacgttt tttcagctcc atagaccccg
    62341 cctggtcgcg gtcaatcacg cggccctgac gttcactggt ggtggaaacc agcatctctt
    62401 cgatgatttc cggcagtgtt tgtgcatttg actccaccgc accggtcagc ggccagcagc
    62461 ccagcgtacg gaaacgcacc atccgttttt taatcacttc gcccggttgc aggtcgatac
    62521 ggttgtcatc aatcatcatc aacataccgt cgcgttccag aaccggacgt tccgcagcga
    62581 gatatagcgg aacaatgtcg atattttcca gccagatgta ttgccagata tcctgctcgg
    62641 tccagttaga gagcgggaag acgcggatgc tttcgccttt gttaatttgc ccgttgtagt
    62701 tgtgccacag ctccgggcgc tgatttttcg gatcccagcg atggaagcgg tcacggaaag
    62761 agtaaattcg ctctttagcg cgggatttct cttcgtcacg gcgcgcacca ccgaaggcgg
    62821 catcaaaacc gtatttgttc agcgcctgtt tcaggccttc agttttcata atatcggtat
    62881 gtttcgcgct gccgtgcacg aatggattaa tccccatcgc cacgccttcc gggtttttat
    62941 gcaccagcag ttcgcagccg taggctttag cagtacgatc gcggaactca tacatctcgc
    63001 ggaatttcca gccggtatcg acatgcagca acgggaaagg cagcgtacct ggataaaacg
    63061 ccttgcgcgc cagatgcagc atgacgctgg aatctttacc gatagagtag agcatcaccg
    63121 gatttgagaa ttctgccgcc acctcgcgaa taatgtggat gctttccgcc tccagttgcc
    63181 gcaggtgagt aagtcgtatt tgatccataa ccgttccttt gcaataccgc tattttcttg
    63241 ccatcagatg tttcgactat agggagcgta agagaacgaa tgaaattacc aattagaatg
    63301 agtagttcct taacggaata acgatttggc aaagctaata tcaaaaagtg cttaaggcac
    63361 cggatttcgg gcgtttagga agatttgaaa ttgttttagc gcagcggcag tttcatacta
    63421 tggcggtaaa aaaatttgca tggtatttaa ggactcacta tgttttccgc attgcgccac
    63481 cgtaccgctg ccctggcgct cggcgtatgc tttattctcc ccgtacacgc ctcgtcacct
    63541 aaacctggcg attttgctaa tactcaggca cgacatattg ctactttctt tccgggacgc
    63601 atgaccggaa ctcctgcaga aatgttatct gccgattata ttcgccaaca gtttcagcaa
    63661 atgggttatc gcagtgatat tcggacattt aatagtcggt atatttatac cgcccgcgat
    63721 aatcgcaaga gctggcataa cgtgacggga agtacggtga ttgccgctca tgaaggcaaa
    63781 gcgccgcagc agatcatcat tatggcgcat ctggatactt acgccccgct gagcgatgct
    63841 gacgccgatg ccaatctcgg cgggcttacg ttacaaggaa tggatgataa cgccgcaggt
    63901 ttaggtgtca tgctggaatt ggcagaacgc ctgaaaaata cgcctaccga gtatggtatt
    63961 cgatttgtgg cgaccagcgg cgaagaggaa gggaaattag gcgcagagaa tttactcaag
    64021 cggatgagtg acaccgaaaa gaaaaatacg ctgctggtga ttaatctcga taacttaatt
    64081 gttggcgata aattgtattt caacagcggt gtaaaaaccc ctgaagcagt aaggaaatta
    64141 acgcgcgaca gggcgctggc aattgcgcgt agtcatggaa ttgccgcaac gaccaatccg
    64201 ggtttgaata aaaattatcc gaaaggcact gggtgttgta atgacgcaga aatattcgac
    64261 aaagcgggca ttgctgtact ttcggtggaa gcgactaact ggaatcttgg gaataaggat
    64321 ggttatcagc aacgcgcaaa aacacctgcc ttcccggcgg gaaatagctg gcatgacgta
    64381 agactggata atcaacaaca tattgataag gcacttcctg gaagaataga acgtcgctgc
    64441 cgtgatgtta tgcggataat gctaccgctg gtgaaggagc tggcgaaggc gtcttgatgg
    64501 gtttgaaaat gggagctggg agttctaccg caggggcggg gaattctaag tgatatccat
    64561 catcgcatcc agtgcgcccg gtttatcccc gctgacgcgg ggaacacaaa accaaacttc
    64621 tccataaatt ccatagccgc ggtttatccc cgctggcgcg gggaacacga gtctatcagc
    64681 gacactaccg gcaatagcga cggtttatcc ccgctggcgc ggggaacacg agagtgctga
    64741 caggtgtctc gattacctga tcggtttatc cccgctggcg cggggaacac ggtagtacgc
    64801 gcctccggac gtttttatgt cgcggtttat ccccgctggc gcggggaaca ctcttcgcgg
    64861 gtaatcaatg atgattcagt ttccggttta tccccgctgg cgcggggaac acgaacgcaa
    64921 tatcacggcg ttctgcggcc tcggcggttt atccccgctg gcgcggggaa caccaaaata
    64981 ttacgagctt cgtcaggcca tggaccggtt tatccccgct ggcgcgggga acacgtcggg
    65041 ccgggtaagt gaacctgtcg gttttccggt ttatccccgc tggcgcgggg aacacactaa
    65101 gcatacataa ctgtttttaa acaaatttat tccgcatcaa caatctacca actaaattaa
    65161 aacatttcct tatttttaaa gaacggataa cttattgatt atcaacagga agaaaagaaa
    65221 ccaaacgtaa cccatccaaa tccaccggaa tacgtctgtt ttctccccag gtctgaaatt
    65281 caaaacccga ctcggtattg gtcgcccagg ccatcaccac atttccgcaa ccagccagtt
    65341 gggtaatttg ctgccagatc atctcccgaa tacgttttga tgtatcacca acatacacac
    65401 cggcacgcac ttccagtagc cagattgcga gccgtccacg taagcgcggc gggacatttt
    65461 ctgtaacaac cacgaccatg ctcatcagcc gcgtcctcga tgaccgctat cgcccaacgt
    65521 ttcaggttcg gggatggctg gcggtaacat atccggcgca ggttgtggcg gttcaatttc
    65581 acctgcggca aggacttcct caattaacgg tattaatttg cccgttaact tagtgctacg
    65641 gaaaatatcg cgacaggcta atctgacttc tttatcaggt tctgcgggtt gcctcgctgc
    65701 tatttcaaat gcctttggca caaccgaatc aaatttaatg atatcggcta tgtcataaac
    65761 aaatgaaagc ggtttgccac tatggataaa cccaatagcg ggcgcatatc ccgcggctaa
    65821 tactgccgct tcagatatac cgtacagaca tgatgtagca gcactgatgc agcgattcac
    65881 aacatcgcct ttttcccagt ctttaggatc gtatttgcga ccattccatt tcacaccata
    65941 ttgtttcgcc agtaatgcat acgtctggcg aacgcgagat ccctcaattc cacgtaactg
    66001 ctccactgaa cggcgagctg gctgtggttc acgaaaacgt aattcataca ttttgcgcac
    66061 caccttcaac cgtagatctt ccgttaaagc cagctttgcc tggtagagta atttatctgc
    66121 ccgcgcccca ccgggttgtc cggaggagta aacgcgaacg cccgcttcac cgacccagac
    66181 cagcagtgtt cccaccgtgg cggccagatg caccgccgcg tgggaaactc tcgttcctgg
    66241 ttcgagcata atgcaggcga ccgatcccac cggaatatgc gttcggatcc ctgttttgtc
    66301 gatcagcacg aaagcgccgt ccagtacgtc gatctgaccg tactggagga agatcatgga
    66361 ggtgcgatct tttaatggga tcgggctcag tggtacaaac gtcacgcctc tgctccgggt
    66421 ttgatcagca tcagaccaca gccgaacgcc cggcttttac catacccctg gctcaggcgt
    66481 tgcacaaata acccaggatc ggtgaccgta agcatccccg tataatccac gctactgaac
    66541 tggatcagtt gccgggagtt ttcccgccgc agttgctgtt gtcggtaggc atcaaccgag
    66601 gtatccagca gtgtaaaacc gcttctctcc ccctgcgctg ccagccagtc cagcgccgcc
    66661 tgttgttgat gcaaccagac atcacttcct tccgccggcc ccctcacctg ccgtttcgcc
    66721 tccatcagca gatcgtggcg cttgcccgct ttacagattg ttggattcgc ccgcaggttg
    66781 aaacaaagtt gttgtccggt acacagctcg ggcgcaaatg accggcattc tatggtgaac
    66841 gtatcacttt ccgccgggcg ttcctgcgac agtacaaaaa agcgaaacgc gccctggagt
    66901 tcttcccgac gataaagaaa ttgcctttct ttgccgccag ggaagagatc ccacagccac
    66961 tgatgcatca catattcacc gcgatccacc agatgcagca actgcgcagg cgaaagctgg
    67021 ccggtatgta aggttattct tgagaggtac atggttcctc cttgctgagc cacggcccct
    67081 gattgacggt gcgttcccca aacagccact gctgacgatt taaaggaaca tcccggcggc
    67141 gtaatatttt gctcgcgacc aggccgtcgt gtttcccttc ccaccagcat tcatcctgaa
    67201 gtttcgggag tgagactttc agtttatgga aatgatcctg atactgttgg tatgcattac
    67261 ataaggcatc acacgcgttg ccttcgagca gtaacggcgc aagaggtaat gccagcggat
    67321 gactttttcg ccccagataa agcggaaaaa ctggatggcg taaaccgtcc tgcaactgtt
    67381 cgaggctgta aggcgcatcg gctgttgttg ccaccgccac catccaccag gcatcggtgt
    67441 agtagtcgcg ccgggagata atcgcgctca aaagctcagg gtcgctcaac tcttcgcgac
    67501 ggctgaaata acgtgcttta cgcacctctt ttggcatctg gaccgtgtga taatcccgtg
    67561 cccagcgcgg gttacggctg gcgcaaacca ccagtgaata gtggcgatta aacgcgttta
    67621 agcgttcggt atcatcacgc cgaatcccta ccccggcagc cagtagcccc agcaatgctg
    67681 agcgcgaagg aagttcatgg gtatgacgaa cttcacctgg agcatcgacc ccccaggacg
    67741 ccatcggccc atgaagctga aaaatcagat attggctcat aagccgcccc ttacgcgcag
    67801 ataaagtcca gcacgtcctt catgcttccc tgtttgttca tcacgtcaaa acttgcgcat
    67861 tcggtcttct gttcgtagac cgtattcatg ttttcgcgaa gcgttgtaat acgctgcacc
    67921 gccacatcta actgacgtgt gccattaatg ggttcataga aagccgccgc cagagagcgt
    67981 ggttgatcgg tgcctttttc tgccagcgcc caggaggcgt aggcacggct ggcaaagctg
    68041 ttctgtttgc cggttgggga gactttaagt gcggcttccg taaaggcacg tatggtctga
    68101 ttagctaacg cttcgtcacc gccaaggttt tcgaccagca gatctttatc aatgcagata
    68161 taggtgtaga acagtgcaga accgaatccg gtttcaccaa gatgcccggc accggcatct
    68221 tcagaagcct ggcgcaaatc atcaacggcg gtgaaaaaat catcttcgac aatcgtttca
    68281 ctgacaccaa atgcgtgcgc tacctggcag gcggcttcaa cattaaactc gggtttattc
    68341 gccagcatac gaccaaacat ggcaatatct accgccatac ggtctttgcg taacaacgcg
    68401 agatcttcct cttttggcgc gcgcttttct tctgcaagct gatgggccag cgcttttacg
    68461 gcgtcaaatt ctgccgggct gatatggact aactgttcag tttcggcgtt agtgagcgga
    68521 tcttttggtt ttttgtcatt tttagctttc ccaagataat cagcaatttt tgccgaccat
    68581 tcgatggctt ttttatcttc aatccctttc tcaatcagga tagttgccgc ctcacgcgca
    68641 atacgcccac tgcgaatacc aatatggccc gccagtgcct gttcaaaaag tgcagaagtg
    68701 cgccacgcac gtttcagact ttgcgaggaa acgcgcagac gcgttgctcc acccagcacc
    68761 acagtttttg gcgcaccggt atcatcacgg ttaaggttgg cagcagggta agcggttaac
    68821 aaatgaagct gaataaatgt tgtcatgaga tagtccttta ttgattttgt tcgttatcgg
    68881 cgtcgccagc ctggtaatat tccagcgccc agcggatacg gataaattcg gttggccgct
    68941 gctggcggcg gtgatgattg agcaggtcgt cgctctcctg gcaccaacgg aagaccccct
    69001 ctgccagaga gtcaagattg acggaaccgt ttaataatct gactgcgcgg cgtaactggc
    69061 gcagtaattc atccggtgtt tttactgccg acagacgagt aaaacgcccc tttgacatta
    69121 ccgccgccag ctgcgcggca aaaggctgcc gttcatcaat agcttttaca ttggcgctaa
    69181 ctgcggcgac cagcgccagt gcggtaaaac gccactcttg tgcttcaatt ttccacaaag
    69241 tgtgggtctg catcagcaga gaacgaaacc cttctgacaa gcaaacatca ttaaccgttg
    69301 tgctacgccg cagacttgca cgattgccgc gtttagcctg caactcttcg cgccatttac
    69361 gcagcgtggc tttgtgctcc tcttttacaa tactcattca gcagcctcct gctttttctc
    69421 cctggcggct ttagcacttt gcttctccgc cgatgttgtg aaatatttct tgcgcgcggt
    69481 cataacgcgt ttcaaatcaa caggctcata tggattggtg aatacacgct cgtcaaaatc
    69541 ctgacgcgcg aataaccaaa tttccttttg ccatttgccg agtaattcgt ccgcatcctg
    69601 accttcttca atttggcgca ctaacctcag gaagcgatgc tgagttttgt tccagaagtc
    69661 gatatccaca aaactgaaat caccccgtgc accttttgga tcggagaacc acgcttcttt
    69721 caatgcactt cgtaacagac ttagaatccg tgaagccgtt tgcgcagcca gacgcagttt
    69781 cggtatctgg ccttcttttt tcttgagcag cagcgggaaa tggtgttcgt accaacagcg
    69841 cgctttcatg ttgtcgaaat catagccaaa tccccacagg cctacttttg cctgtttcag
    69901 actgctggca ttaaagagtt tcaccaccag cgcgggaagt tccgtattgt tttctgattt
    69961 acccgtttcg ataaggccta accagtcgcg ccagattaaa ccgcccggtt gtggtttgac
    70021 cgagtaaaac tcgccaccct ctttaagtgg tatacggtaa ggcgttaagg gatgctgcca
    70081 catggcataa ttcgcaccgt aatttttggt cgtcatcagg ctcaggagcg cgtcgctctg
    70141 ttcaccgcaa atatcgcagt tgccgactgt cgtggtatta aaatcaatac gaatacggcg
    70201 cggcattccc cagtaagcct ggagtttatt gacctgatca tgggttacca ccgcaccggc
    70261 cagttcgctg gttcgcgtcg ggccaagcca ggggaaaacc agatcgtcaa attttttggg
    70321 tagcggtaag tcggcttcat cctgcggcat cacattgggc cacagtttgc gccacaaggg
    70381 cgtttgttga ttgccttgat attcctgtaa ttcaatcaga gtggtcatcg gcccaccgcc
    70441 gcgcaaaccg gtgcgatagc ctttgccgcc tgatggcgca tttaactgta gggagaacag
    70501 agctaacgcg gaacaatgag ggcatacgtg ttcagtcacg ccacgcttaa taaagtggtc
    70561 tttattaaac ttcgttgttt gagcgccggg aatctcaggc agtagcgaag cgacctgaac
    70621 tttatctccc ttgagcgcct cgaaatcctg cataaatgaa ggggaatctg ccccaaactg
    70681 gaaagcgtgt tctaaggaca gcaatgcttc ccgtagcttt tcagcctcca gtccgtcttg
    70741 ccagatatca tcccaacgac cgtgattttt gggcgcgaag ctggtttgta gtagccccag
    70801 caaaaactgc caggccgccc cctggagatc tgcccgcggc gcagcgatat cgacaacatt
    70861 ttcatcagcc agatcgactg gtgccagctt gcctgtcgtt ccgtctttaa aacgaacagg
    70921 caaccacggg gttgtcagaa gtgaaaacga gttcatacgc atcctctcgt aaaaaagacc
    70981 atcccaggtc ggttgtccat ctttccatcc ttttcggtgt atcaactatt cagagagtcc
    71041 tgtcgcagac atctcaacca accttgccaa tcaatccttc cgtagccgaa tacccgcaac
    71101 tttcgtcatc gttgactaaa atcacgtttg ccatctccgg atcttgtcgc tgttcaatgc
    71161 accacagcct gaacgcctct ccctccagta aagaaaactc atcccgatgt tttttccacc
    71221 agcttcggcg cactctgaca acgctcattt cccatgcgtg agcaccggtg gcataaggct
    71281 tcaccacacc gtcaatacag gtagccagcc acagggaaac agactcctca gccagacgtg
    71341 tcgacagttt ttccgggagg taatcgttga tattggcggc atagccgggc ctgaagttca
    71401 gcacaaactt tttagccatt gcgcgatcgc agtaatattt gcccacttgc tcctgctcgc
    71461 tgcgggcaaa tccttccggc attaccacgt cctcaccgta gacggattca ataagaagcc
    71521 gggctgcgtg tggcatctga atagcacctt gctcacgcag cacacgctgc gtcagccaga
    71581 ttcgcccatg atcgggatag acaaatgcac tgttacgcat ggcactgcca aaccattcgt
    71641 cgccaggaga gtcgtcccag acgggagcca gaatcagcaa ttcagggggg gaacgctcgt
    71701 cctttccgtc acgctttaac tgaccattaa tatcgcggat atgccgctgt aatcgtcctg
    71761 ctcgctgaat cagcaaatca acaggggcca ggtcggagat catttcgtcc aggtcaatat
    71821 ccacgctttg ctcaattact tgtgtacaaa caatgacttg cgacgctctc tgtaaactac
    71881 aatatttacc aaagcgtgcc agcgtttccg tttcaattcg ctggcgatcg ctaaaagcaa
    71941 agcggctatg aaaaagtgaa aggctggaag caggaatgac gccgcgggca agcagctgac
    72001 gatgaacctt aatagcgtca tcgacagaat tccggatcca ggcgatgcat ttcccctgac
    72061 ttaccgccga ttcgatacgc gcaatacact cttgttcact atgaagccaa cccacgctga
    72121 cgctacgctc aacatctttg cgcgtcgcta cccggtgtga gttcacatcg gatttcgtga
    72181 catgcgtcag ccaggggtaa tcatcctttt ccaggaacgg agcttcttgc tggccctctg
    72241 taccccgcgc aaaggcagcg acgagtttgt cgcgctgctg ttgagataac gtggcagaaa
    72301 gcaaaataac gctgtttccg ccacgcgcct gccgctcgat caacccttca agaatgcacg
    72361 acatgtaagc gtcacaggcg tggatctcat cagccagcag gattttgtta ctcagcccca
    72421 gaagccgcag attattatgt ttgaacggca tcaccgccat catcgcctga tccagcgtgc
    72481 cgacgccaat ttcagccagc aacgccttct tgttactgtc ggcaaaccag gccgcacatc
    72541 cctgactgaa cgtttgttca tccggttctt ctgacccgac taaatcacca gaccagagtg
    72601 attcattaaa gcggtccatt aatgtgcggg cactatgtgc cagcaccagg ctggggcggg
    72661 attctggcga atagaaagca agccaggttt tgaccagccg atcgtacatg gcattggccg
    72721 ttgccattgt tggcagacca aaaaacagcc cctgagcttt ccccgcagct atcaacctgt
    72781 gcgccaggat aagcgccgct tctgttttac ctgcgccagt cacatcttcc agaataaata
    72841 actgcggtcc ttgctggctg atatccagat ccagcacctt ttgctgtaac ggtgtcggat
    72901 tctcaataaa aggaaacagc gtattaattc cgttgaaagg cgcgactttt gcttttagag
    72961 gaaagacggt taaggcgttt tgagcctgaa ttaaagtttt ctgccagtaa cctttaatat
    73021 ccattggatg tgcgacgcgt ggaaaaaatc gcgttgacga acccgtccag tctgcgagca
    73081 cgacggttgc agagatatac caggaaaggt gttttataag ttcaatgccc tcatcatcat
    73141 cccagaatgc ggggatctct atgagcggaa acagtggttt gatttcaagg agaaaatctc
    73201 gtgctgcagc tttgtcttca ggcagaaaat tatccaattc atccatacga tcagggggct
    73261 gaccatgatg ccctgtcgtc acgggcatcc acatttctat cactcgccta agtttgcgcg
    73321 gagagagcga agatgaagga agtaactcct gacattcact taaataatga ttccacaacc
    73381 agtagcccag cgttgaatga gagatttttt cgtaattttt tctggcacct tcctgaatct
    73441 tgagttcagg gggcaggtaa agttgttgaa aagagcgggc aaattttcca atatcgtgcc
    73501 agcacagcaa ccaggcgaaa aattgagccg cctgttcctt gtcagaaatc cctaattgac
    73561 gaaaataatc agccagcccg aagcaatttc ttttaaccat taaatagccc atcgcggcta
    73621 catccagcga atgccagcaa agaaggtgat agtcgtcgcc cccgtctttc tcgccgcgtc
    73681 gggtttttcc ccagaaatca
//
LOCUS       DAZGOB010000007        67274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGOB010000007 DAZGOB010000000
VERSION     DAZGOB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.007"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..917
                     /locus_tag="ACHLIF_000478"
     CDS             3..917
                     /locus_tag="ACHLIF_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO9604548.1"
                     /translation="MFYQPWVNALPPMPRHANPEQLEKTVRYLTQTVHPRSADNIDNL
                     NRSAEYIKEVFVSSGARITSQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSASSYE
                     NDELTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPPFFRSDEMGSAV
                     HAASLERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVGRMQDINAV
                     RQVKATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYHL
                     PGDTADRLNYQKMAQVVDGVINLLYNSK"
     gene            1020..1523
                     /locus_tag="ACHLIF_000479"
     CDS             1020..1523
                     /locus_tag="ACHLIF_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO9604549.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1590..3047)
                     /gene="nuoN"
                     /locus_tag="ACHLIF_000480"
     CDS             complement(1590..3047)
                     /gene="nuoN"
                     /locus_tag="ACHLIF_000480"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO9604550.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3054..4583)
                     /gene="nuoM"
                     /locus_tag="ACHLIF_000481"
     CDS             complement(3054..4583)
                     /gene="nuoM"
                     /locus_tag="ACHLIF_000481"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO9604551.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFDISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSRSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4747..6588)
                     /gene="nuoL"
                     /locus_tag="ACHLIF_000482"
     CDS             complement(4747..6588)
                     /gene="nuoL"
                     /locus_tag="ACHLIF_000482"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO9604552.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLVT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6585..6887)
                     /gene="nuoK"
                     /locus_tag="ACHLIF_000483"
     CDS             complement(6585..6887)
                     /gene="nuoK"
                     /locus_tag="ACHLIF_000483"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO9604553.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6884..7438)
                     /gene="nuoJ"
                     /locus_tag="ACHLIF_000484"
     CDS             complement(6884..7438)
                     /gene="nuoJ"
                     /locus_tag="ACHLIF_000484"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO9604554.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7450..7992)
                     /gene="nuoI"
                     /locus_tag="ACHLIF_000485"
     CDS             complement(7450..7992)
                     /gene="nuoI"
                     /locus_tag="ACHLIF_000485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO9604555.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8007..8984)
                     /gene="nuoH"
                     /locus_tag="ACHLIF_000486"
     CDS             complement(8007..8984)
                     /gene="nuoH"
                     /locus_tag="ACHLIF_000486"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO9604556.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8981..11707)
                     /gene="nuoG"
                     /locus_tag="ACHLIF_000487"
     CDS             complement(8981..11707)
                     /gene="nuoG"
                     /locus_tag="ACHLIF_000487"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO9604557.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11760..13097)
                     /gene="nuoF"
                     /locus_tag="ACHLIF_000488"
     CDS             complement(11760..13097)
                     /gene="nuoF"
                     /locus_tag="ACHLIF_000488"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO9604558.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13094..13594)
                     /gene="nuoE"
                     /locus_tag="ACHLIF_000489"
     CDS             complement(13094..13594)
                     /gene="nuoE"
                     /locus_tag="ACHLIF_000489"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO9604559.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13597..15399)
                     /gene="nuoC"
                     /locus_tag="ACHLIF_000490"
     CDS             complement(13597..15399)
                     /gene="nuoC"
                     /locus_tag="ACHLIF_000490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO9604560.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15493..16155)
                     /gene="nuoB"
                     /locus_tag="ACHLIF_000491"
     CDS             complement(15493..16155)
                     /gene="nuoB"
                     /locus_tag="ACHLIF_000491"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO9604561.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16171..16614)
                     /gene="nuoA"
                     /locus_tag="ACHLIF_000492"
     CDS             complement(16171..16614)
                     /gene="nuoA"
                     /locus_tag="ACHLIF_000492"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO9604562.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17245..18183)
                     /gene="lrhA"
                     /locus_tag="ACHLIF_000493"
     CDS             complement(17245..18183)
                     /gene="lrhA"
                     /locus_tag="ACHLIF_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO9604563.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19103..20320
                     /gene="alaA"
                     /locus_tag="ACHLIF_000494"
     CDS             19103..20320
                     /gene="alaA"
                     /locus_tag="ACHLIF_000494"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO9604564.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20404..21003
                     /gene="yfbR"
                     /locus_tag="ACHLIF_000495"
     CDS             20404..21003
                     /gene="yfbR"
                     /locus_tag="ACHLIF_000495"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO9604565.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21062..22894)
                     /locus_tag="ACHLIF_000496"
     CDS             complement(21062..22894)
                     /locus_tag="ACHLIF_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO9604566.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22981..23631)
                     /gene="hxpA"
                     /locus_tag="ACHLIF_000497"
     CDS             complement(22981..23631)
                     /gene="hxpA"
                     /locus_tag="ACHLIF_000497"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO9604567.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23642..24136)
                     /locus_tag="ACHLIF_000498"
     CDS             complement(23642..24136)
                     /locus_tag="ACHLIF_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO9604568.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24219..24674)
                     /gene="yfbV"
                     /locus_tag="ACHLIF_000499"
     CDS             complement(24219..24674)
                     /gene="yfbV"
                     /locus_tag="ACHLIF_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO9604569.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25012..26214
                     /gene="ackA"
                     /locus_tag="ACHLIF_000500"
     CDS             25012..26214
                     /gene="ackA"
                     /locus_tag="ACHLIF_000500"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO9604570.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26289..28433
                     /gene="pta"
                     /locus_tag="ACHLIF_000501"
     CDS             26289..28433
                     /gene="pta"
                     /locus_tag="ACHLIF_000501"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO9604571.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28623..30143
                     /gene="yfcC"
                     /locus_tag="ACHLIF_000502"
     CDS             28623..30143
                     /gene="yfcC"
                     /locus_tag="ACHLIF_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO9604572.1"
                     /translation="MSATTESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30176..30718)
                     /gene="yfcD"
                     /locus_tag="ACHLIF_000503"
     CDS             complement(30176..30718)
                     /gene="yfcD"
                     /locus_tag="ACHLIF_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO9604573.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30776..31327)
                     /gene="yfcE"
                     /locus_tag="ACHLIF_000504"
     CDS             complement(30776..31327)
                     /gene="yfcE"
                     /locus_tag="ACHLIF_000504"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO9604574.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31383..32027)
                     /gene="yfcF"
                     /locus_tag="ACHLIF_000505"
     CDS             complement(31383..32027)
                     /gene="yfcF"
                     /locus_tag="ACHLIF_000505"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO9604575.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32163..32810
                     /gene="yfcG"
                     /locus_tag="ACHLIF_000506"
     CDS             32163..32810
                     /gene="yfcG"
                     /locus_tag="ACHLIF_000506"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO9604576.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32867..33229
                     /gene="folX"
                     /locus_tag="ACHLIF_000507"
     CDS             32867..33229
                     /gene="folX"
                     /locus_tag="ACHLIF_000507"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO9604577.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33250..34143
                     /locus_tag="ACHLIF_000508"
     CDS             33250..34143
                     /locus_tag="ACHLIF_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO9604578.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34191..35081)
                     /gene="rpnB"
                     /locus_tag="ACHLIF_000509"
     CDS             complement(34191..35081)
                     /gene="rpnB"
                     /locus_tag="ACHLIF_000509"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO9604579.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35278..36051)
                     /gene="hisP"
                     /locus_tag="ACHLIF_000510"
     CDS             complement(35278..36051)
                     /gene="hisP"
                     /locus_tag="ACHLIF_000510"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO9604580.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36059..36775)
                     /locus_tag="ACHLIF_000511"
     CDS             complement(36059..36775)
                     /locus_tag="ACHLIF_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9604581.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36772..37458)
                     /gene="hisQ"
                     /locus_tag="ACHLIF_000512"
     CDS             complement(36772..37458)
                     /gene="hisQ"
                     /locus_tag="ACHLIF_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO9604582.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37548..38330)
                     /gene="hisJ"
                     /locus_tag="ACHLIF_000513"
     CDS             complement(37548..38330)
                     /gene="hisJ"
                     /locus_tag="ACHLIF_000513"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO9604583.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38551..39333)
                     /gene="argT"
                     /locus_tag="ACHLIF_000514"
     CDS             complement(38551..39333)
                     /gene="argT"
                     /locus_tag="ACHLIF_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO9604584.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39599..40168)
                     /gene="ubiX"
                     /locus_tag="ACHLIF_000515"
     CDS             complement(39599..40168)
                     /gene="ubiX"
                     /locus_tag="ACHLIF_000515"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO9604585.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40263..41780)
                     /gene="purF"
                     /locus_tag="ACHLIF_000516"
     CDS             complement(40263..41780)
                     /gene="purF"
                     /locus_tag="ACHLIF_000516"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO9604586.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41817..42305)
                     /gene="cvpA"
                     /locus_tag="ACHLIF_000517"
     CDS             complement(41817..42305)
                     /gene="cvpA"
                     /locus_tag="ACHLIF_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO9604587.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42564..43226)
                     /gene="dedD"
                     /locus_tag="ACHLIF_000518"
     CDS             complement(42564..43226)
                     /gene="dedD"
                     /locus_tag="ACHLIF_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO9604588.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKSKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43216..44484)
                     /gene="folC"
                     /locus_tag="ACHLIF_000519"
     CDS             complement(43216..44484)
                     /gene="folC"
                     /locus_tag="ACHLIF_000519"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO9604589.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44554..45468)
                     /gene="accD"
                     /locus_tag="ACHLIF_000520"
     CDS             complement(44554..45468)
                     /gene="accD"
                     /locus_tag="ACHLIF_000520"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO9604590.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45624..46283)
                     /locus_tag="ACHLIF_000521"
     CDS             complement(45624..46283)
                     /locus_tag="ACHLIF_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9604591.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46366..47178)
                     /gene="truA"
                     /locus_tag="ACHLIF_000522"
     CDS             complement(46366..47178)
                     /gene="truA"
                     /locus_tag="ACHLIF_000522"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO9604592.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVMRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTRAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47178..48191)
                     /locus_tag="ACHLIF_000523"
     CDS             complement(47178..48191)
                     /locus_tag="ACHLIF_000523"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO9604593.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48257..49393)
                     /gene="pdxB"
                     /locus_tag="ACHLIF_000524"
     CDS             complement(48257..49393)
                     /gene="pdxB"
                     /locus_tag="ACHLIF_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO9604594.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49492..50487
                     /gene="flk"
                     /locus_tag="ACHLIF_000525"
     CDS             49492..50487
                     /gene="flk"
                     /locus_tag="ACHLIF_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO9604595.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50484..51662)
                     /locus_tag="ACHLIF_000526"
     CDS             complement(50484..51662)
                     /locus_tag="ACHLIF_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9604596.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51946..53166)
                     /gene="fabB"
                     /locus_tag="ACHLIF_000527"
     CDS             complement(51946..53166)
                     /gene="fabB"
                     /locus_tag="ACHLIF_000527"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO9604597.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53325..55331
                     /gene="mnmC"
                     /locus_tag="ACHLIF_000528"
     CDS             53325..55331
                     /gene="mnmC"
                     /locus_tag="ACHLIF_000528"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO9604598.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFNQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55452..55730)
                     /locus_tag="ACHLIF_000529"
     CDS             complement(55452..55730)
                     /locus_tag="ACHLIF_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO9604599.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55764..56312)
                     /gene="epmC"
                     /locus_tag="ACHLIF_000530"
     CDS             complement(55764..56312)
                     /gene="epmC"
                     /locus_tag="ACHLIF_000530"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO9604600.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56312..57121)
                     /locus_tag="ACHLIF_000531"
     CDS             complement(56312..57121)
                     /locus_tag="ACHLIF_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO9604601.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(57121..57945)
                     /gene="mepA"
                     /locus_tag="ACHLIF_000532"
     CDS             complement(57121..57945)
                     /gene="mepA"
                     /locus_tag="ACHLIF_000532"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO9604602.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSSLWKPEI
                     FSLIKLAAQDKNVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57949..59034)
                     /gene="aroC"
                     /locus_tag="ACHLIF_000533"
     CDS             complement(57949..59034)
                     /gene="aroC"
                     /locus_tag="ACHLIF_000533"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO9604603.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59069..60001)
                     /gene="prmB"
                     /locus_tag="ACHLIF_000534"
     CDS             complement(59069..60001)
                     /gene="prmB"
                     /locus_tag="ACHLIF_000534"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO9604604.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            60167..60718
                     /gene="smrB"
                     /locus_tag="ACHLIF_000535"
     CDS             60167..60718
                     /gene="smrB"
                     /locus_tag="ACHLIF_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO9604605.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60791..61642)
                     /locus_tag="ACHLIF_000536"
     CDS             complement(60791..61642)
                     /locus_tag="ACHLIF_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO9604606.1"
                     /translation="MHKIRHVICLLVLALCASGIQAATKIATLYVPAGTTSVVAKYRF
                     HLSVLTPQSVEYGTYESNSTAAASLPLVSWTGSPPGPELRMERNNTTLPDSTCPGLEE
                     YDALSPVTAWSCNELVLGVYYDGDLHGCPWIVSSYVESASTMDQRFGAGIFIGPTKVS
                     SDCPTESLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCDGSNFDER
                     GAYCRFVAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
     gene            complement(61644..62183)
                     /locus_tag="ACHLIF_000537"
     CDS             complement(61644..62183)
                     /locus_tag="ACHLIF_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9604607.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(62180..62668)
                     /locus_tag="ACHLIF_000538"
     CDS             complement(62180..62668)
                     /locus_tag="ACHLIF_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9604608.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(62665..63174)
                     /locus_tag="ACHLIF_000539"
     CDS             complement(62665..63174)
                     /locus_tag="ACHLIF_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9604609.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(63190..63942)
                     /locus_tag="ACHLIF_000540"
     CDS             complement(63190..63942)
                     /locus_tag="ACHLIF_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9604610.1"
                     /translation="MFNLTNTAKIVVPALALLATVVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(63962..66607)
                     /gene="yfcU"
                     /locus_tag="ACHLIF_000541"
     CDS             complement(63962..66607)
                     /gene="yfcU"
                     /locus_tag="ACHLIF_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO9604611.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHIEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDVGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(66689..67252)
                     /locus_tag="ACHLIF_000542"
     CDS             complement(66689..67252)
                     /locus_tag="ACHLIF_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9604612.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16548 a        18344 c        16947 g        15435 t
ORIGIN      
        1 ttatttttta ccaaccctgg gtcaatgcat taccgccaat gccccgacac gcaaatcctg
       61 agcaattaga aaaaacagtg cgttatctta cacaaactgt gcatccacgt agtgccgaca
      121 atattgataa tctgaatagg tccgcagagt atataaaaga ggtcttcgtc agtagcggtg
      181 ccagaattac ctcgcaggac gtccccatta cgggtggccc ctacaaaaac attgttgctg
      241 attatggtcc tgccgatgga ccgctgatta ttattggtgc gcactatgac tctgccagca
      301 gttatgaaaa cgatgaattg acctatacac ctggcgcgga tgataacgcc agcggcgtgg
      361 caggattact cgaactggca cgtttgttac atcagcaagt accgaaaaca ggcgtgcagc
      421 tggtcgccta tgcgtcggaa gaaccgccct tctttcgtag cgatgaaatg gggagcgcgg
      481 tacatgcagc ttcgcttgag cgtccagtaa aattaatgat agcactggag atgattggct
      541 attacgactc tgcgcctggt agccaggatt acccttaccc ggcaatgtcc tggctttatc
      601 ccgatcgggg agactttatt gccgtggtcg gcagaatgca ggatatcaac gccgttcgtc
      661 aggtaaaagc gacattgtta tcatcccggt atttatctgt ttattctatg aatgccccag
      721 ggtttattcc cggtattgat ttctctgatc accttaatta ttggcaacac gatattcccg
      781 ccgtaatgat tactgacacc gctttttatc gtaataaaca ataccacttg cccggtgata
      841 ccgcagacag attgaattat cagaaaatgg ctcaggtggt ggatggtgtt ataaatttgt
      901 tatacaacag taaataataa aaatttctcg aggcgggtcc agaaaaagcg acatgcacat
      961 tttattttcc ctcgtctgtt ttaagatgcg aaacatgaaa ttcaatggag gaaaaataaa
     1021 tgggaatgat tggttatttt gcagaaattg actcagaaaa gattaaccaa ctgcttgagt
     1081 ctactgaaaa acctttgatg gataatatac atgacacgct ttcaggatta cgccgactgg
     1141 atattgataa acgctgggat tttttacact ttgggctgac aggcacctct gcttttgatc
     1201 ccgcgaagaa tgatccgctg agtcgtgccg tgcttggtga acacagcctc gaagatggca
     1261 tagatggctt cctgggattg acctggaatc aggagttagc cgcgacaatc gaccgactgg
     1321 aatcactgga tcgaagtgag ctgcgtaaac agttttccat taagcgactc aatgaaatgg
     1381 agatttatcc gggagtgacc ttttcagaag agttagaggg gcaactcttt gcttcgatca
     1441 tgcttgatat ggaaaagctg attagcgcgt atcgacgtat gctgcgacaa gggaatcatg
     1501 cgctgacggt tattgttggg tgatggtgtc agggcaccaa aaaaaaccgc cgaacatgtc
     1561 ggcggctttc tgacttacaa actaacagat tacatcagcg gcattgctaa ccgcacaatg
     1621 ctaatcagcg gttgtggcca tacacccagc accagtacca acagtgcaga aatcagcacc
     1681 acgataccgc ccgcgctgta ctgccagttt gatggtgcat cgcgacccgg ttgttccggg
     1741 gcgtgaagat acaggctcac cgccacgcgc aggtagtagt agaggccgat tgccgaaccg
     1801 acaaccacgg cacccaccag ccaccacaag tgtgcctgga caccgaccgc cagcacgtag
     1861 aacttaccga taaagcccag cgtcatcggg ataccggcca gagacagcat catcaccgtc
     1921 atcactgccg cgaggatcgg acgatgccag aacagaccgc ggtaagagaa cagggaatca
     1981 gcatccgggc cacgatacgg gctggacatc aggctgacca cgccgaacgc gccgaggctg
     2041 ctgaacagat aaccggccag gtaaacccct accgcttcca tcgacatctc accagtttgc
     2101 agcgcaatca gcgccaccag cagatagccg aggtgagaga tagatgagta accgagcaga
     2161 cgtttgatgt tggtctggct cagcgccatc aggttaccga agatgatgga ggcaaaggcg
     2221 ataatcgcca gtactacgcg aatcgcttcg ctgtcaccca ccggtgcgta gaggaacaga
     2281 cgcatcacca caccgaagat agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc
     2341 gcaggcgcgc cctggtatac gtctggcgtc cacaggtgga acggcaccag cgagagtttg
     2401 aagccgaggc caacaatcat cagtccgaaa cctgccagca acagcggctc gttgagcata
     2461 ccgtcgccga ggtttttacc cagcgcgaca aacgacaggt cgccagactg tgcatacacc
     2521 agcgccatac caaacagcag gaaagaagac gctgcggcag aaaggatggt gtatttgata
     2581 ctggcttcca gcgaacgttt ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa
     2641 gagatcagtt caataccgag gaacaaagac gccagatggt tggcattcgc cagcaggatc
     2701 ccgcccagcg cggcaattaa caccaacagg tagaactcat ccttgttgtc gttatagcct
     2761 tcaagccacg ggtaggcgaa agtacaggtg gcgaggctcg ccaacaatac cagcccggtg
     2821 taaagcatgg cgaaaccatc aacgcgcatc agcggcgtaa cgtccatagc gcccgcctgg
     2881 ccgacaaacc agagcgaaac cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg
     2941 aggaaatgat tgcgtcgcca cgcaatggag agcatcacaa ccaccaccgt caagccgacg
     3001 atcagcaacg gtagcagtgc gatcaggttt tgtggagtta ttgtcatggc gatttacggc
     3061 cttgtagtag taacggaatt aacaaaccac tgctggatat tgccaatcgc ggagcgcgag
     3121 gtatccagaa tcggctgcgg atagaagccc agcagtacca gcagcaccac cagcaacagg
     3181 atcataaaca gctcacgcag cgacatccct ggcagttcct ggctggcaat ctggcttttc
     3241 gctttaccga agtaagcgcg atgcaacatc gccagcgaat aaacagatgc aaagaccagc
     3301 ccaaaggtag agataacggt aatcaccggg acaacctgga agctgccgaa cagaatcata
     3361 aattcgccga cgaagttacc ggtgccaggc atcccaagcg ttgccaccgc aaagaacagc
     3421 gacagtgctg gcagccattt catcttgctc cacagaccgc ccatcatgcg catgtcgcgg
     3481 gtatggatac gttcataaag ctgaccacaa agaataaaca gacccgccgc cgacaagccg
     3541 tgcgcaatca tctggattac cgcgccctgg taggccaact ggctgccggt gtagatagca
     3601 atcagcacga agcccatgtg ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc
     3661 tgggcgaagg ccatccacgc accgtagaag atgccgataa cacccagcca catagcaatt
     3721 ggcgcgaact ctgccgacgc gttcgggaac agcggcaggg agaaacgcag caaaccgtaa
     3781 gcggcagttt tcagcaagat ccccgcgagg tcaacggaac cggcggtcgg agcctgggag
     3841 tgcgcatccg gcagccagcc atgcagcgga accaccggca ttttgactgc gaaggcgatg
     3901 aagaagccca gcattaacag gtattccaca ccactggaca ttggcgtatt cagcagctct
     3961 tcatagttga aggtccagac gccggtcgca ttgtagtgaa caaaaaccag cgccaggatg
     4021 gcaatcaaca tcaccaggcc actcgcctgg gtgtaaatga agaacttggt tgccgccgtg
     4081 atacgcgttt taccgtcaga ggctttatgc ccccacagtg cgatcaggaa gtacatcggc
     4141 accagcatca tttcccagaa gaagaagaac aggaacatgt cgatggcaag gaacacgccg
     4201 ataacgccgc ccaggatcca catcaggttg aggtggaaga agccctgata tttttcgatc
     4261 tctttccacg aacatagtac cgccagcaca ccgagcagac cggtcagcac gaccatcagc
     4321 agcgacagcc cgtcaatggc gagatgaata gagatatcaa aacgcgggat ccacggcatg
     4381 tcgaattcag actgccactg cggaattccg gcggattgcg tcagtgaata accgccctgc
     4441 aaccacagtt gcagcgacag cgccagcgtc aatcccatgg tgatcagcgc gatccagcgt
     4501 ggcaccttga cgccaaagcg ttcggtctgc cagcacagga agccgccaat aaagggaatt
     4561 aatattagcc agggtagtaa catggcgatc tttattcctt gtaaaagtcc cgtcaggacc
     4621 ggattttcaa cgaattctca cgacagaaaa ttctcttcgg actgggggta gtgtcggatg
     4681 caccgcgaag gcggcttatc cgacctacgt tcttatatgc ccaggggcaa tcccacaatc
     4741 cttaactcaa cgcagtacca tcaacagtgc cagcaccacg accgcaccga tgctcatgga
     4801 tgccacatac cagcgcagat agccgttctc acttaacagc agacctttac ctgcaaagcg
     4861 ggaaaggaca gccgggatgt tcatcattga gttcagcgga tcgcgtttca gcaaccaggc
     4921 aatacccagg aacggcttga cgaacacttt gtcatacagc cagtcaaatc cccaggcgtt
     4981 gtaccaccag gtgcccagca gacggcccgg cgcactgttg gcgatggagg tcaccagagt
     5041 acgtttaccc agccacagcc aggctgccag cagaatgccg accaccgcga ccacgccaga
     5101 ggtaatttcc agggtcaaca tgctgccgtg cgccagttcc gtcgtttgcg gaagcacgcc
     5161 ctgcagcggc ggtacaatca gtgcgccaac gaaggtggaa aggatcagca gcacaatcag
     5221 cggcaggctg tgagttaccc ctttcacggc gtgagcgtga atttgttctt ttccgtggaa
     5281 gacgatgaaa atcatacgga aggtgtagag cgaggtcata aacgcaccga ccagacctgc
     5341 caccatcaga ttgatatgac cattcgccat cgcacccgcg aggatctcat ccttactgaa
     5401 gaagcccgca gtgaccagcg gtagtgccga cagtgctgcg ccgcccacca ggaagcagag
     5461 ataaaccagc ggaatagatt tacgcagacc gcccatcttg aagatgttct gttcgtgatg
     5521 gcaggccaga atgacggaac cggatgccag gaacagcagc gctttaaaga acgcgtgagt
     5581 catcaagtgg aaaatcgccg catcccatgc ctgcacgcca agcgcgagga acatgtagcc
     5641 aatctggctc atggtagagt aagcgagaac acgtttgatg tcggtctgta ccagcgcggc
     5701 aaaaccggcc agcagcagcg taaccgcccc gacaataccc accagatgca gaacttccgg
     5761 cgtcaccagg aacaggccgt gggtacgggc gatcaggtag acacccgcgg ttaccatcgt
     5821 tgcggcgtgg atcagcgcgg agacaggcgt cgggcccgcc atcgcgtcag caagccatgt
     5881 ctgcaacggc aactgcgcag atttaccgac cgcaccgccc agcagcatca gcgtcgccca
     5941 catcagcatg ttattgccgt cagcaaagtg cgctggtgcc agttccacca tttcgcggaa
     6001 gttcagggtg cccagttcgt tgtaaagaat gaacagtgcg aaagcgagga aaacgtcacc
     6061 cacacgggtc acgacgaacg ctttcattgc cgctgcgcca ttcttcggat cggtgtaata
     6121 gaaaccgatc agcagatagg agcacaggcc cacgccttcc cagccgaggt acatcagcag
     6181 caggttatcg gcgagaacca gaactaccat gctggcgatg aacaggttgg tgtaggcgaa
     6241 gaagcgagag tagccctctt caccgcgcat gtaccaggag gcgtacatgt gaataaggaa
     6301 acccacacca gtgaccaccg agagcatggt cagcgacagg ccgtccagca ccaggttaaa
     6361 accgatgtta aagtcgccta ccgacatcca cgtccacagc ggctggctgt atgactgctc
     6421 gccgttagcg aagaaatcaa cgccgataaa ggcggttacc agcgccgcca ggcccacaga
     6481 gccaacgccg acgatcgccg aaacgttttc agaccagcgc ccacgggaga acgccagcag
     6541 gacgaagcca atcaatggca aaataatggt taaggcaagc atgttcatcc gcgcatctca
     6601 cttactgaat cgatgttcag gttctggcga cgacggtgaa gttgcagcag cagcgcaagg
     6661 ccgatactcg cttctgccgc cgcgaggctg atggcgagaa tgtacatcac ctgaccgtcg
     6721 gtctgacccc agtagcttcc ggcgaccaca aaggccagcg cggaggcgtt aatcatgatt
     6781 tccagaccaa tcagcataaa cagcagattg cgacggataa ccagaccggt taagccaaga
     6841 acgaataaga ttgccgcgag gatcagtcca tgttgtaagg ggatcatgcg tgctcctccg
     6901 tttttctttt cgcgctgtcg tctttacgat tgctcagcac ttcacccgca cgctcttcac
     6961 gaccgacgtg gaaggccaca accagacccg cgagcagcag catagaagcc agttccaccg
     7021 ccagtacgta aggcccgaac agcgtaatac ccactgcttt agcactgatt ggcgtaccgt
     7081 cgataccctg atcattaaca ccaaggatgg cgtaaacaat caccaccagc atgatggccg
     7141 acaaaattgc cggaccaatc cacacctgcg gtttcagcca ctggcgttcc tgttcgattt
     7201 ctgaaccgcc caggttgagc atcatcacca cgaacacgaa cagcaccata atggcacccg
     7261 cgtagacgat aatttccagc gcaccggcga agtaagcgcc cagtgagaag aacaccccgg
     7321 aaatcgccag cagcgaaata atcaggtaca gcagtgcgtg taccggattg gtatgggtga
     7381 tcactcgcaa ggtcgcaagt atggctatca ggccacagat ataaaaagcg aactccattg
     7441 cccctctcct tacggtaaca ggctcttgac gtcgataggc ttggcttcgt tctctgcttc
     7501 gcccttatct ttgccgtcga ttgccatacc tgccatccgg tagaagttat attccgggta
     7561 tttgcccgga ccggagatca gcagatcctc tttttcgtaa accagatcct ggcgcttgta
     7621 ttcccccatt tcgaaatccg gggttaactg aatcgccgtg gtcggacagg cttcttcgca
     7681 cagaccacag aaaatgcagc gtgagaagtt gatgcggaaa aattccgggt accagcgacc
     7741 gtctttggtt tctgcttttt gcagcgagat acagccgacc gggcaggcta ccgcgcagag
     7801 gttacaggct acgcaacgct cttcgccgtc cgggtcgcgg gtcagaacga tacgaccacg
     7861 ataacggggc ggcagataga ccggctcttc cgggtacatt cgcgtttcgc gtttggcgaa
     7921 cgcgtgcagg ccgatcatcc agatactacg aacctgggtg ccgaaaccta ctaacaattc
     7981 ttttaaggtc atggtcttat tgccccttat tgcgcctgcc agagaatgac agccgccgtt
     8041 accagcaagt tgatcagcgt cagcggcagg cagattttcc agccgaagga cattacctgg
     8101 tcataacgcg gacgcggtaa cgacgcacga atcaaaatga acatcatcat aaagaacgcg
     8161 gttttcagcg cgaaccagat gaatggcggt aacaacgggc cttgccagcc accgaagaac
     8221 agcgtcacca tcaatgcaga gatggtcaca atcccgatgt actcacccac gaagaacaga
     8281 ccgaacttca taccggaata ttcaatgtgg taaccatccg ccagttcctg ctcggcttcc
     8341 ggctggtcaa acgggtgacg gtgacatacc gccacgcccg cgatggcaaa ggtaataaaa
     8401 ccaaagaatt gcgggataac gttccacaca tgcgcctggc tgttgacgat gtcggtcatg
     8461 ttgaatgaac cggcctgcgc caccacgccc atcagggaaa gcccgaggaa cacttcgtag
     8521 ctcagggtct gcgcagaagc acgcatcgca cccagcaacg agtatttgtt gttacttgac
     8581 cagcccgcaa acagcaccgc gtaaaccgcc agacctgcca tcatcaggaa gaacaaaatc
     8641 ccgatgttca ggtcggcaac cacccaaccc ggactgactg gcacaatcgc aaaggccagc
     8701 agcagcgagg taaaggcaat catcggtgcc agggtaaaga tgacgcgatc cgagaatttc
     8761 gggatccagt cttctttaaa gaacattttg atcatgtccg caaccagctg gagcgaaccg
     8821 ccccagccaa cacggttagg tccgtaacgg ttctggaaca gacccagcag gcgacgttcg
     8881 ccaaagctca tgaatgcccc gcaggtgaca accaccagca ggatcaccac cgctttgagg
     8941 atggtcagca ggatctcaat cagttccggt gatatccaac tcattgttgt gcctccttga
     9001 gatcctcaag atgcgcgcca gccagcaccg gagcaatgcc ggacataccc atcggcaaac
     9061 ccacctgccc tgccgtcagc ccttcagcga tttcaaccgg cagcgtgacc gtgttgccat
     9121 cgtaactaaa ggagacgcga gcgcctgcgt tcacgcccaa cttcgcggca tccgctgggt
     9181 tgagtttgat gtacggctgc ggcatacggc tctggaagac cggagcacgc tgtgacaatt
     9241 catcgctgcc aaacaggtgg taatacggcg cgatacgcca tttcccgtcc tgcggctgga
     9301 agcgtgccgg tacgctggtg aagtaatcca gaccattttc gctggtttca aacagacgca
     9361 cgcccggatc gccaaagcgc agtttgccgc ccacttcgtc ctggaatttg ttccacgcct
     9421 gcggggagtt ccagcccggc gcccaggcaa acggcacttg cgaacggtgc gcagtcggct
     9481 ggttgttacc ttccatcgag aaggtgaaca tggtgtcaat atcctgcggc tgacgcggct
     9541 catgaacgct gatattggcg cgcatggcgg tacgaccgct gtaacggtgc ggttcacggg
     9601 ccagtttctg cccacgaata cggaatgtcg catccggcgc agcatctttg atacctgcca
     9661 gttccgggat tttcgccaca acagcgtcaa tcacatggtc gagctgcgtc cagtccactt
     9721 cacggctcag cagggtgctg tgcagcgagt gtaaccagcg ccagctttcc agcatgacag
     9781 ttttgctgtc gtaataagca ggatcgtaaa cctggaagaa acgttgggcg cggccttcgt
     9841 tgttgatcac cgtaccgtcg ctttcagcaa agctggcagc agaaagtacc agatgggcgt
     9901 tttccataat cgctgtgcgt tgatgatcaa ccaccatcac cagcggcgct ttagccagcg
     9961 cagcattcac gcgggtagcg gaagcgtgac gatgcagatc gttttccagc accaccaccg
    10021 cgtcggcgcg tccggtttcc agttcggtca acgcttcttc aagcgaaccg ccacccataa
    10081 tgcccagccc catgctgttg acggaacggg caatcatggt gataccaacg tcagcgccgc
    10141 gacctttcag ggctttcgcg acgttagccg cagcctgaat cacctctatg ctaccggcgt
    10201 tcgtcccgga gataatcaac ggtttcttcg cacctgccag tgcctgcacg atgacgtcga
    10261 ttttgctttg cagctcaggt tcgataccgt caaccgctgg cgcagagtta tccagcgcat
    10321 gggcgatggc aaaacctaaa cgcgcctgat cttcaaccgg tgcgcggtaa gtccacgccg
    10381 cgatatcatc cagacgggtg tcatcaacgt tggtaacaaa cagcggatgc ttcgcacgtt
    10441 gaccgatgtt gaggattgcc gcaatctgcc agtcagccac tttctgtgct gccgccattt
    10501 cgcgcgcttt acctttcaca gcctgacgca ctgccagcgc gacgcgcgcg ccggtctggg
    10561 taacgtcttc gcccagcacc agtaccgcat cgtaagattc gatttcgcgc agagccggag
    10621 tataaatgcc gccttcacgc agcactttca gcgccagttg cagacgttcc tgctcaccgt
    10681 gagcgatacc ggtgtagaag ttttcttcgc ccaccagttc acgcagcgca aagttgcttt
    10741 ccacgctggc acgcggagaa ccaataccga tcactttctt cgactgacgc agaatatctg
    10801 ccgcgccctg cattgcctgt tcggcgttga gggtaatgaa atcatcgcca cgacgctgta
    10861 ctggctgacg cggacgatcc ttcaggttga cgtaaccgta accgaaacga ccacggtcgc
    10921 agaggaagta gtggtttacc gtaccgttgt aacggttttc gatacgacgc agttcgccgt
    10981 aacgttcacc ggggctgatg ttacagccga tggaacattg ctggcagatg ctcggcgcaa
    11041 actgcatatc ccatttacgg ttgtaacgct cggagtgcgt tttgtcggta aatacgccgg
    11101 tcgggcaaat ttcgaccagg ttaccggaaa attcgctttc cagcgtgccg tcttccgggc
    11161 gaccgaagta gacgttgtcg tgcgcaccgt aaacgcccag atctgtaccg tcagcgtaat
    11221 ctttgtagta acgcacacag cggtagcagg cgatgcagcg gttcatttcg tgagagatga
    11281 atggccccaa atcctgatta cggtgggtac gtttggtgaa acggtagcga cggaagctgt
    11341 gtccggtcat cacggtcata tcctgaagat ggcagttacc gccttcttca cataccggac
    11401 agtcgtgcgg gtggttggtc attaaccact cgaccacact ttcacggaac tgtttcgctt
    11461 cttcgtcgtc aatggaaata aaggtgccat cggaagccgg tgtcatacag gacatcacca
    11521 ggcgaccacg cgtgtcttcc gcgttttggt attgcttcac cgcacactgg cggcaagcac
    11581 cgacgcttcc cagcgccgga tgccagcaaa agtaaggaat atcaaggccc agagacagac
    11641 aagcttccag caggttgtcc gctccgttga cctcgtattc tttgccgtct acatgaattg
    11701 tagccattag catgcttcca gttttctcag tcagagactg agcgttaatc gaaattcggt
    11761 taccagcgct ctttcagcag gttcggctga atcccattaa tcaaatgggt attgctgaac
    11821 ggctgtttga ttcccgcctc aaattcttcg cggaaatatt tgatggcgct ctgtaacggc
    11881 tccactgcac caggtgcgtg ggcacagaaa gttttacccg ggcctaagaa tcgacacagt
    11941 tgctcaagtg tttcgatatc gcccggctga ccttcaccac gctccagcgc acgcagaatt
    12001 ttcacgctcc acggcagacc gtcgcggcac ggcgtacacc agccgcagga ttcacgggcg
    12061 aaaaactctt ccaggttacg caccagcgac accatgttga tctcatggtc aaccgccatc
    12121 gccagcgccg tacccagacg gcttcctgct ttaccgatac tttcgaattc catcggcagg
    12181 tcaagatgcg cttcggtcag gaagtcggtg cccgcgccgc ctggctgcca ggctttgaat
    12241 ttcagaccgt cacgcatacc accggcgtaa tcttcgagga tctcgcgcgc ggtagtacca
    12301 aacggcagtt cccacaggcc cggatttttc acccgaccgg agaagcccat cagcttggtg
    12361 ccagcatctt tacttttcga gatgttctga taccactcca cgccgttagc gaggatcgcc
    12421 ggaacgttac acagggtttc gacgttgttg acacaggtcg gtttacccca tgcgccggag
    12481 gtcgccggga atggaggctt cgagcgtggg ttagcacgac gtccttccag ggagttgatt
    12541 aacgctgttt cttccccgca gatgtagcgc cctgccccgg tatggacgaa cagttcgaag
    12601 tcaaaacctg ttcccataat atttttgcca agcagacctg cttcggtggc ttcggcaatg
    12661 gcacggcgta gattcactgc cgcttcgata tattcgccac gcaggaagat gtagccacgg
    12721 taagctttca gcgcaaacgc ggagatgagc ataccttcca ccagcaggtg cggcagttgc
    12781 tccatcaaca ggcggtcttt ataggtgccc ggctccattt catcggcgtt acacagcagg
    12841 taacggatgt tcatggattc gtctttcggc atcaggctcc acttcaggcc agtcgaaaag
    12901 cccgcgccgc cgcgcccttt cagaccagcg tcttttacct gattaacgat ttcgtccgga
    12961 gacagcccgg tcagcgcctt acgcgcccct tcgtaaccat ttttgctgcg gtattcgtcc
    13021 agccacactg gctgtttgtc atcgcgcaga cgccaggtca gcggatgcgt ttcgggagta
    13081 cggataatgt ttttcattta taccgctcca gcagttcagg gatcgcttcc ggggtcagat
    13141 gcgcgtgagt gtcctcatcg atcatcatat ttggcccttt atcacagttc cccaggcagc
    13201 aggttggcag cagcgtaaag cgaccgtcaa acgtcgtttg ccctggtttg atgttcagtt
    13261 ttttctccag cgccgcctga ataccctggt aaccgttgat gtgacagacc acgctgtcgc
    13321 aataacggat cacatggcga ccaaccggtt ggcggaagat ctggctatag aacgttgcca
    13381 cgccttcgac atcgctcgcc ggaataccca gcacatcggc gatcgcgtgg atcgcgccgt
    13441 ccggcaccca gccacgctgc ttctgaacga ttttcagcgc ttcaatggac gccgcacgcg
    13501 ggtcttcgta gtggtgcatc tcgtgctcaa tcgcttcacg ctctgccgca ctcagctcaa
    13561 aagcctcggt ttgtggttgt tgattctcgt gcataattag cggtccacat ctgacataac
    13621 aaaatcgata ctgcccagat aaacaatcag gtcagacacc aggctgccgc ggatcgccgc
    13681 cggaatttgc tgcaaatgcg cataactcgg cgtgcggata cgagtgcgat aactcatggt
    13741 gctaccgtcg ctggtcaggt agtaactgtt gatccccttg gtcgcctcaa tcatctggaa
    13801 agattcattg gcaggcatca ccggccccca cgacacttgc aggaagtggg tgatcagggt
    13861 ttcgatatgt tgcagcgtgc gctctttcgg cggcggcgtg gtcagcgggt gatccgcttt
    13921 gaacgggcct tccggcatgt tgttgaggca ctgctcaaga atgcgcagac tctggcgcag
    13981 ctcttccact ttcagcatta cgcgggtgta gcagtcagaa acaccaccac caaccgggat
    14041 ttcaaagtcg aagttttcat agccagaata aggacgcgcc ttacgcacgt cgaagtcgat
    14101 cccggtagca cgcaggcccg cgccagtggt gccccactcc agcgcctctt tcgcgccata
    14161 ggcggcaacg ccctgggaac gacctttcag aatggtattt tgcagcgccg ctttctcgta
    14221 agacgccaga cgtttcggca tccagtcgag gaactcacgc agcaggcgat cccagccgcg
    14281 cggcaggtcg tgcgctacgc cgccaatacg gaaccacgcc gggtgcatac ggaaacccgt
    14341 gatcgcttcc accagatcgt aaattttctg acgatcggta aaggcgaaga acacgggcgt
    14401 cattgcgccg acgtcctgaa taaaggtcga gatgtacagc aggtggctgt tgatacggaa
    14461 cagttcggag agcataacgc gaatgacgtt aacgcgatcc ggcacggtga tcccggccag
    14521 tttctctacc gccagcacgt aaggcatttc gttaacgcag ccgccgaggt attcaatacg
    14581 gtcggtatac ggaatgtagc tgtgccagga ctggcgctcg cccattttct ccgcaccacg
    14641 gtggtggtag ccgatgtctg gtacgcagtc gacaatctct tcgccgtcga gttgtaaaac
    14701 gatacggaaa gccccgtgcg ccgacgggtg gttcggaccg aggttgagga acatgaagtc
    14761 ctcgttttcg gtgccgcgct tcatccccca ctcttccggt ttgaaggtca gggcttccat
    14821 ctccagatcc tgtttggctt tggtcagctc aaacggcgag aattcggtag cgcgcgccgg
    14881 gtaatcttta cgcagcgggt gacctttcca ggtttgcggc atcatgatgc ggcgcaggtt
    14941 cgggtgaccg tcgaaagtaa tgccaaacag atcccaggtt tcacgctcat accagttagc
    15001 gttcgggaac agtttggtga aggtcggtac gtgcaggtcg ttttctgcca gcgccacctt
    15061 cagcatgatg tcgcggttac gatcgataga aatcagatgg tagaaaacgg aaaaatccgc
    15121 ggcaggtaac ccttcgcggt gtgtgcgcag acgttcgtcc atgccgtgta agtcaaacag
    15181 catgacgtaa ggtttcggca gtttctttaa gaaatcgcca acttccagta attgttcacg
    15241 cttgatccac acaacgggaa ccccggtgcg agtcgcctga acagtaaagg catccggccc
    15301 aaaacggttg cgcagttcgc caattaccgg atcatcaaga tgatcgcggg tctgccaggc
    15361 gggttcttgc gcggttaagt cggtcatatt gttcaccatt gcaaatggtc cgtggtgact
    15421 gtcggcgcag taagcttcgc gaaatagatt taggaataag cgaagaaaaa tccctttgcc
    15481 gacaggcgca aattaaatct cgtcaggtgt acgcaggttg gttacggcaa tacgttcacc
    15541 gcgcttgcgt tcgcgctctg attgcatatt ggcgcgataa acgccctgat cgccaaccac
    15601 ccaggagagc ggacgacgtt ctttgccgat agattcctgc aacagcatca gtgcctgcat
    15661 gtacgcttca ggacgcggcg ggcagcccgg gatatacaca tcaaccggga tgaatttatc
    15721 gacgccctgc acaacggaat aaatatcgta cataccacca gagttggcac aggcacccat
    15781 tgagataacc cattttggtt ccagcatctg gtcatacaga cgctgaataa ccggtgccat
    15841 tttggtaaag caggttcctg caaccaccat caggtcagcc tgacgcggcg aagcacgcaa
    15901 tacttctgcg ccaaaacgcg ccacgtcatg caccgcggta aacgaagtca ccatctcaac
    15961 gtaacagcag gaaagaccga agttatacgg ccaaattgag tttttacgac cccagttaac
    16021 catgtcattg agcttgccca taaacacgtt tttgttaact tcttgctcca gagggtcggt
    16081 tacgatctcc tgcttttgca gggggtaacg gtcgttctca ccgttgggat ctatgcgggt
    16141 gagcgtataa tccatcttaa tgcctcgcgg ttagcgttga cgattagcga tactgttcgt
    16201 ttccgggttc atacgctcgc ggcgtgaacg cgcgggcgtc cagtccagcg cgccaatacg
    16261 caccagataa accagacctg ccagtaacac aaaaataaaa attgcagctt ccacaaagcc
    16321 tacccagccg ctttcgcgga tagaggttga ccatgcgaac agatacagcg cttcaacgtc
    16381 gaagataacg aagaacatgg ccaccagata aaacttggcg gacaggcgta agcgggcgga
    16441 gccgaccgag tcgataccgg attcaaacgg cacgtttttc gacctcgcgc gtgcgcgacc
    16501 gcctaaaaac caaccgccta ccagcatcag gcaacacagg ccaatggcaa cgataagaaa
    16561 gatagcgaat gcccagtgat gagcgatgac ttcagtggat gttgacatac tcattgctta
    16621 ctcatcaaaa gtagcgccag attcactgct cttcacggca gatggacgcc acatcgattc
    16681 atggggagga ataaaaaaaa ccttacaatc actgtagaaa ttcttttata cagctaattg
    16741 atgtggtctt ttactccttt ctataacctt ttgtcaactt taacaaaagt ttcttcacat
    16801 tagtttacat aatatcaaca ccattagcat ttaatgccct ttcaccccag atccttgacg
    16861 actccaggat aattagatgt tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg
    16921 ggtctatttt gacaggaatt tgtgtcgatt cctcccccca aaagagagta ttttcttgat
    16981 ctgtgacacg cttttgtcat tccataacaa aaacgcagca acaaatttac gtatttttta
    17041 acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg acgtgaatct
    17101 ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa ggatttttac
    17161 taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgttttttac actattgtct
    17221 caggaattat ctatcgtccg tcgattactc gatatccctt tcaatcaaca atgaatcatc
    17281 cccttccgga gcagacattg ggctgtattg ccacggatta tggtagctct ccatcgcctg
    17341 atagatcacc tgggccagct cattattact ggacgggtca tagcacagca aatattcggt
    17401 atcaggtagc ggcggtaagc catctactcc actcagcaca cgcaggtccg ggctcatcat
    17461 ctcaaccggc cttgccgtca cgccaagacc ggctttcact gccgcacgaa cggccggaag
    17521 cgtcgaggcg acataagcca gtcgccatgg aatatctgct ttattaagcg tcgccagcac
    17581 catatcgcga aacggacttg gatcatccag caatacaaga gggatcggct cgcctttttg
    17641 caatacgtat tccgctgcgc agtaccagtg tgttggcgag gttcgcaggt tcaatgcctt
    17701 aaacgctgaa ggacgatggg tggttaccat caaatccact tcctgggatt ccagcatttc
    17761 tgccatatag gcattacgtt taacgcggac atccagcgct aatttcggat aaaccgaact
    17821 cacgcgattt aacaggaaag gtaagatcgt atcggcagat tcatctgaag caccgatagt
    17881 taacacgccc tgaagattac tgaacattaa tgatgagcag gcctcatcat taaaacgcag
    17941 gattttcctg gcgtaaccaa gaagttgaat gccatgttca gttaacagtt tgttgcgacc
    18001 gtgacgagcg aacagttctt tcccaacgag ttgttccaga cgctgcattt gctgacttac
    18061 ggcggactga gtacgacaca cagcggcagc tgcggcagca aaagtgttca gatcggcaac
    18121 agcaacaaat gttctcagca gatcgaggtc gaggttaatt atcggacgat ttgcacttat
    18181 catatattat cacttactgg cggctcatac tgagctggtt aatgctgtgc acacacaaac
    18241 aagcaattcc atttgtaatg tgcctccctg gcagtttcat ccggaacccg gacgaaagta
    18301 aaaatgcata tgagttgcac taaaaaagcg actcacattg ttccgttata atgcctgaag
    18361 tagatcacag aatatatctt cagggatcgc atatctatta agttactcac tcttttctat
    18421 ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc cttgttccac tctcgtgcag
    18481 catcgctggt catacgcgaa cacgtacaaa cagcaatggt gaggcggcat caagagcagg
    18541 gatccgtcat ttatgcgagc attttacccc aaaaaccttt tatttataag ggtcattgcg
    18601 aattatctga tgcaaagtta tgttatgtta ggcaaagtaa tcttctatta ttaataagca
    18661 catcaaaacc tttttgaata ttaaataata attaattagc atcatcctca ttcattaatt
    18721 ccgcttaaca atagtttcac accttgcccc cattacgaca cctcggaaat catcacgctg
    18781 ttaaactctc cgtaatgcgg aaatatcatc atcgcgtttc attagtgaat tcttaaatga
    18841 ggcattttca cacaattatc ttacagataa aaaaaccaga cttacaatta agaatcagaa
    18901 caattcacta tataacattg catgtaaagc atatacacct cattattttg tcattattaa
    18961 gttattaaca gcacaatcga gccttcccct ctggcaaaat cttattctgc agaccttcaa
    19021 aacaccgtcc tgggggagta cattgttcta agctgacttc cacggcaggg agtggcgata
    19081 acagcaaaaa aggtcaagat tcatgtcccc cattgaaaaa tccagcaaat tagagaatgt
    19141 ctgttatgac atccgtggtc cggtgctgaa agaagcaaaa cgcctggaag aagaaggtaa
    19201 caaggtactg aaactgaaca tcggcaaccc agccccgttc ggttttgacg cgccagatga
    19261 aatcctcgtt gacgtgatac gcaacctgcc taccgctcaa gggtattgcg attccaaagg
    19321 tctttactcc gcgcgtaaag ccatcatgca gcactaccag gctcgtggca tgcgtgatgt
    19381 taccgtggaa gatatttaca tcggcaatgg tgtatcggag cttatcgttc aggcaatgca
    19441 ggcattgctg aacagcgggg acgaaatgtt ggttcctgca ccagattacc cactatggac
    19501 cgcggcggtt tcgctttcca gcggtaaagc ggtgcattat ctttgcgatg aatcctctga
    19561 ctggttcccg gacctcgatg atattcgcgc taaaattacg cctcgtacgc gtgggatcgt
    19621 tattatcaac ccaaataacc caaccggcgc ggtatattcc aaagagcttt taatggagat
    19681 tgtggagatt gcacgtcagc ataatctcat tatcttcgcc gatgaaattt atgacaaaat
    19741 tctttacgac gacgctgagc atcactcaat tgcgccgctg gcacctgacc tgctgaccat
    19801 tacctttaac ggactgtcga aaacgtaccg cgttgcaggc ttccgtcagg ggtggatggt
    19861 gttgaacggg ccgaaaaaac acgccaaagg ctacatcgaa ggtctggaaa tgctggcttc
    19921 aatgcgcctg tgtgctaacg ttcctgcgca acacgccatt cagaccgcgc tgggtggtta
    19981 tcagagcatc agtgaattta ttacccctgg cggtcgtctt tatgagcagc gtaaccgcgc
    20041 gtgggaactg atcaacgata ttccgggcgt ttcctgcgtg aaacctcgtg gtgcgctgta
    20101 tatgttcccg aaaatcgacg ccaaacgctt taacattcac gacgatcaga aaatggtgct
    20161 ggatttcctg ttgcaggaaa aagttctgtt ggtgcaaggg acggcattca actggccgtg
    20221 gccggatcac ttccgcattg tcacgctacc gcgtgtcgat gatatcgagc tgtctttgag
    20281 caagttcgcg cgtttccttt ctggttatca tcagctgtaa tcttaatttc actgccggag
    20341 attgcatccg gcagcgttat cccgccacaa tgacctgatg atgtcatcat acgtaaggtc
    20401 actatgaaac agagccattt ttttgcccat ctctcccgcc tgaaactcat taaccgctgg
    20461 ccgctcatgc gcaacgtgcg gacggaaaat gtgtccgaac acagtttgca ggtagcgatg
    20521 gtcgcccatg cgctggcagc tatcaaaaat cggaaatttg gcggtaatgt caacgccgaa
    20581 cgtatcgctt tactggcgat gtaccacgat gcctcagaag tgctcaccgg cgatctccct
    20641 actccggtga aatacttcaa ttcgcaaatc gctcaggagt acaaggctat tgaaaaaatc
    20701 gctcagcaaa aactggtcga tatggttccg gaagagctgc gggatatctt tgcgccgtta
    20761 attgacgagc atgcatatag cgatgaagaa aaatcgctgg tgaaacaggc agatgcactg
    20821 tgtgcatatc tgaaatgtct ggaagaactc gcggccggaa ataatgaatt cttgctggca
    20881 aaaacgcgac tggaagcgac gcttgaagcg cgtcgcagcc aggagatgga ctacttcatg
    20941 gaagtatttg ttcccagctt ccatctttcg ctcgatgaga ttagccagga ttcaccgctg
    21001 taagcagccg gagtctgcgt cgcatcaggc aataagcgcc ggatgcgaca tcaggctctt
    21061 gtcaaaacgg aaacagcatc gggatcatca ccacacaaac cgccatcacg ataatggtga
    21121 acggtacccc caacttcaca aagtcactga agctgtaatt tcccggacct aaaaccagtg
    21181 tgttaacagg tgaagaaacc ggtgtcataa aggcggcgga tgctgccatc gccacgacca
    21241 tcgcgaatgg ataaggcgac acccccatcg ttttggcagc agccagcgca atcggagcca
    21301 tcaacaccgc cgtcgcggta ttagagataa atagcccaat aaccgccgac aagacaaaca
    21361 aacaccccag catcatatgt ggcccgtaac caccgccaat gtccatcagc cctttcaccg
    21421 ccagcgcgac acctcccgtt ttctgtaatg ccacagcaaa cggcatcatc ccaacgatca
    21481 aaataatgct cggccagtga atggatttat aggcgctttc agcatctata cagcggaatt
    21541 tccccatcag caggcaggcg atgatagcgg caacaggatt aggaatttca tctgtcagca
    21601 ttaacgccac cattagcacc agacagaaaa tggcatgggg tgcctggctg tgcgcgggcg
    21661 atgcttcact cacctcttcc ggtaagttca gcgctacgaa gtcgcggccc tgtttggcca
    21721 gcataccgat cagtttccag ttacccacaa ccaggatgat atcgcccagc agcagaggct
    21781 catccgccag cgaaccttcc agcgccacgc cattgcgctt tagccccacc acattcagtc
    21841 cgtagcgggt acgaaaacca atttcgcgca ccgatttacc aatcagttct gactcaggaa
    21901 ttaatgaaat ctctgccatg cccacatcaa gggcctggtc agaaaaatac tcgccgcgca
    21961 gtaccatcgg ctccagcaat tgctcactac aaaattgccg gagatcgaca tcagccgcag
    22021 acatatcaat aagcaaaacg tcacgcgcgc gaaattcaga aaccccatta acgttcacga
    22081 taacgcgacg aaaacgccgc cagcgttcaa caccgatgac gttagcgcca taacgctcac
    22141 gtaatttgag atcatccagc cgttgaccta tcattggcga tccggggcga atagccagac
    22201 gtcgcgcacg cccggtcagt cgatattcac ggataagatc gcgaaaggtt cgacgcgtcc
    22261 agccttcgcg ctgcggggtc tgggtatccc ctttcagcat gaaacgcatc actaacatat
    22321 acaaaatacc cagcaccagc acgaccaggc caataggtgt tacgctaaag aaactgaagc
    22381 cgtgatagcc ttcacgcagc aattcactgt tgactaccag gttcggcggc gtcgccacca
    22441 gcgtcatcat gccgctaatc agcccggcaa aacttaacgg catcatcaga cgtgacggcg
    22501 aagtttgcat acgcatggca acgcttaaca ccacgggaat aaagatagcg acaacgccgg
    22561 ttgaactcat aaacgcgcca agccccgcga cggtcagcat caacaaaacc aacattttga
    22621 tttcactgtt gcccgccact ttgaccagcc atgttcccat tacggtggca acaccggtac
    22681 ggaccaaacc atcgccaata ataaacaagg cggcaatcag gacaacgtta ggatcagaaa
    22741 agccggaaaa tacttcgggg accgtcagcg ttccgcttaa tgcaaacgcg acaataacaa
    22801 acaaagcgac cgcatccata cgcactctgc ccgtcgcaaa caagacgatg gcaacggcca
    22861 gtaatgaaag aacccaaatc aattcaccgt tcacaactta tccttgttaa ttgaggggga
    22921 tgacttgatt ctgccataaa aaagccccga cgagacgggg ctaaatcatg atcaggtgtt
    22981 tcactgaata ataacatcgc catttggctg tttggtcaca gtaatttgct ccagactgtg
    23041 gaggaccaaa tcgacctcat tcaggcgcgg ggtatctgcc ggagcgttaa ccgcaatgac
    23101 atggcaaccc gccgccaggc cagaaagcac gccagcggga gcatcttcca ccaccacaca
    23161 ctcctgcggc gcaagcccca gcagctgcgc acctaacaga tacgcatcag gttctggttt
    23221 tccgcgcttc actcgctcag cggttacaaa cacctctggt gcgggaagcc cagctatttt
    23281 atggcgcgct cgcgctaccg gcatggagcc agaagtcaca atggcccacg gaatacctgc
    23341 tttattcaaa tgattgagta aggcgattgc ccccggaagc gcggtaatac cttcggtttc
    23401 cgtggcctcg atgtgctcca gacgcgtaaa ctcggcggca atatcagcct cggatttgcc
    23461 cgccataaaa tggcgcaaag aggtgatcgc ctgtttaccg tgaatgaaag ccagcacctc
    23521 ttccggcgct aacccatgac gtctggccca gttgctccac gcccgttcta ccgcaggcag
    23581 ggaatccacc agcgttccat caagatcaaa cagaaaacct ttgcaccgca cgcgggcctc
    23641 ctcaggcgtt gataatttga ttaatttcgt tggcgctcaa atggtactga cgcgggcagg
    23701 catgccacac attaagcatg cgctgatatt tttcccacat tggcgtctgg gcgttaaaac
    23761 cgtgagttcc ggcgtcaaaa tgggtatagc gcccttccac attaaccata aagcggacat
    23821 aaccgaggta acgtgcttca gtggcggcgt caaagccgag gaaggtgaca cgacgttcat
    23881 cgatggattg ctggtcctgc aaattagacc aggaaacatg caacgcatga tacatctcca
    23941 taatgtcgat gatagtgcgg caggtttctt ctttcagctc gccaaactcg cgatccaatt
    24001 cacgcatctg taatccgtaa ccacgctcaa taattgtttg caggcgacgg taacgttcag
    24061 catttgccgg atcgagcata gtcatcatct tgtactgatt agacaaaata agacgttgcg
    24121 cgttggtcat ttccattgtt gactcctgta tcactatact acggtgaaaa aaaagaaggc
    24181 tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt acaaatcatc aaggaaagtt
    24241 ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta atgcctggta atcaggcttg
    24301 ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt taccgcgaac ttcataaaac
    24361 cagttaagga ttgcaggggg taatggcgtt acagaacgct tgcccagcca ccacaatccc
    24421 tgcatgggta aacttaaggc gaacagcgca gtggcaactg ccggcccaag ctgaccgccc
    24481 agggcaatct gccagcagag agtaaatacg gcgatcggcg gcataaaacg gatcgcataa
    24541 cgcgtcatct tgataacgcg attttcgaca aagaccgggg caaggcgttt ttccagcggc
    24601 cacgtctttg agtaatgctg tccccggcga aacaagctaa aaaaattaac agaacgatta
    24661 tccggcgttg acatgcttca cctcaacttc acatataaag attcaaaaat ttgtgcaaat
    24721 tcacaactca gcgggacaac gttcaaaaca ttttgtcttc catacccact atcaggtatc
    24781 ctttagcagc ctgaaggcct aagtagtaca tattcattga gtcgtcaaat tcatatacat
    24841 tatgccattg gctgaaaatt acgcaaaatg gcatagactc aagatatttc ttccatcatg
    24901 caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca tgcgctacgc
    24961 tctatggctc cctgacgttt ttttagccac gtatcaatta taggtacttc catgtcgagt
    25021 aagttagtac tggttctgaa ctgcggtagt tcttcactga aatttgccat catcgatgca
    25081 gtaaatggtg aagagtacct ttctggttta gccgaatgtt tccacctgcc cgaagcacgt
    25141 atcaaatgga aaatggacgg caataaacag gaagcggctt taggtgcagg cgccgctcac
    25201 agcgaagcgc tcaactttat cgttaatact attctggcac aaaaaccaga actgtctgcg
    25261 cagctgactg ctatcggtca ccgtatcgta cacggcggcg aaaagtatac cagctccgta
    25321 gtgatcgatg agtctgttat tcagggtatc aaagatgcag cttcttttgc accgctgcac
    25381 aacccggctc acctgatcgg tatcgaagaa gctctgaaat ctttcccaca gctgaaagac
    25441 aaaaacgttg ctgtatttga caccgcgttc caccagacta tgccggaaga gtcttacctc
    25501 tacgccctgc cgtacaacct gtacaaagag cacggcatcc gtcgttacgg cgcgcacggc
    25561 accagccact tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc ggtagaagaa
    25621 ctgaacatca tcacctgcca cctgggcaac ggtggttccg tttctgctat ccgcaacggt
    25681 aaatgcgttg acacctctat gggcctgacc ccgctggaag gtctggtcat gggtacccgt
    25741 tctggtgata tcgatccggc gatcatcttc cacctgcacg acaccctggg catgagcgtt
    25801 gacgcaatca acaaactgct gaccaaagag tctggcctgc tgggtctgac cgaagtgacc
    25861 agcgactgcc gctatgttga agacaactac gcgacgaaag aagacgcgaa gcgcgcaatg
    25921 gacgtttact gccaccgcct ggcgaaatac atcggtgcct acactgcgct gatggatggt
    25981 cgtctggacg ctgttgtatt cactggtggt atcggtgaaa atgccgcgat ggttcgtgaa
    26041 ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg atcatgaacg caacctggct
    26101 gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc ggtggttatc
    26161 ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc ctgatttcac
    26221 accgccagct cagctggcgg tgctgttttg taacccgcca aatcggcggt aacgaaagag
    26281 gataaaccgt gtcccgtatt attatgctga tccctaccgg aaccagcgtc ggtctgacca
    26341 gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg agcgttttca
    26401 aacctatcgc tcagccgcgt accggtggcg atgcgcccga tcagactacg actatcgtgc
    26461 gtgcgaactc ttccaccacg acggccgctg aaccgctgaa aatgagctac gttgaaggtc
    26521 tgctttccag caatcagaaa gatgtgctga tggaagagat cgtcgcaaac taccacgcta
    26581 acaccaaaga cgctgaagtc gttctggttg aaggtctggt cccgacacgt aagcaccagt
    26641 ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc gtcttcgtta
    26701 tgtctcaggg cactgacacc ccggaacagc tgaaagagcg tatcgaactg acccgcaaca
    26761 gcttcggcgg tgccaaaaac accaacatca ccggcgttat cgtcaacaaa ctgaacgcac
    26821 cggttgatga acagggtcgt actcgcccag atctgtccga gattttcgac gactcttcca
    26881 aagctaaagt aaacaatgtt gatccggcga agctgcaaga atccagcccg ctgccggttc
    26941 tcggcgctgt gccgtggagc tttgacctga tcgcgactcg tgcgatcgat atggctcgcc
    27001 acctgaatgc gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt aaatccgtca
    27061 ctttctgcgc acgcagcatt ccgcacatgc tggaacactt ccgtgccggt tctctgctgg
    27121 tgacttccgc agaccgtcct gacgtgctgg tggccgcttg cctggcagcc atgaacggcg
    27181 tagaaatcgg tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc atttctaaac
    27241 tgtgcgaacg tgctttcgct accggcctgc cggtatttat ggtgaacacc aacacctggc
    27301 agacctctct gagcctgcag agcttcaacc tggaagttcc ggttgacgat cacgagcgta
    27361 tcgagaaagt tcaggaatac gttgctaact acatcaacgc tgactggatc gaatctctga
    27421 ctgccacttc tgagcgcagc cgtcgtctgt ctccgcctgc gttccgttat cagctgactg
    27481 aacttgcgcg caaagcgggc aaacgtatcg tactgccgga aggtgacgaa ccgcgtaccg
    27541 ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg ctgggtaatc
    27601 cggcagagat caaccgtgtt gcagcgtctc agggtgtaga actgggtgca gggattgaaa
    27661 tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg tctggtcgaa ctgcgtaaga
    27721 acaaaggcat gaccgaaacc gttgcccgcg aacagctgga agacaacgtg gtgctcggta
    27781 cgctgatgct ggaacaggat gaagttgatg gtctggtttc cggtgctgtt cacactaccg
    27841 caaacaccat ccgtccgccg ctgcagctga tcaaaactgc accgggcagc tccctggtat
    27901 cttccgtgtt cttcatgctg ctgccggaac aggtttacgt ttacggtgac tgtgcgatca
    27961 acccggatcc gaccgctgaa cagctggcag aaatcgcgat tcagtccgct gattccgctg
    28021 cggccttcgg tatcgaaccg cgcgttgcta tgctctccta ctccaccggt acttctggtg
    28081 caggtagcga cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa aaacgtcctg
    28141 acctgatgat cgacggtccg ctgcagtacg acgctgcggt aatggctgac gttgcgaaat
    28201 ccaaagcgcc gaactctccg gttgcaggtc gcgctaccgt gttcatcttc ccggatctga
    28261 acaccggtaa caccacctac aaagcggtac agcgttctgc cgacctgatc tccatcgggc
    28321 cgatgctgca gggtatgcgc aagccggtta acgacctgtc ccgtggcgca ctggttgacg
    28381 atatcgtcta caccatcgcg ctgactgcga ttcagtctgc acagcagcag taatctcgtc
    28441 atcatccgca gctttgcgct gcggatatct gaaccggaaa taatcactat ttccggtttt
    28501 ttattctctt aatctgcatt aatcctttct gattatcttg cttaactgcg ctgcatcaat
    28561 gaattgcgcc atttcacttt gcatacttac cactttgttt tgtgcaaggg aatatttgcg
    28621 ctatgtccgc aaccactgaa tccaaaccaa caagaagatg ggcaatgccc gatacgttgg
    28681 tgattatctt ttttgttgct attttaacca gccttgccac ctgggtagtt ccggtgggga
    28741 tgtttgacag tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca cgcaaagtcg
    28801 tagatccaca ctcatttcgc attctgacta acgaagcagg cgaacctgag tatcaccgcg
    28861 tacagctgtt cacgacgggc gatgaacgcc cgggcctgat gaacttcccg tttgaaggat
    28921 taacctcagg atcgaaatac gggacagctg ttggcatcat catgtttatg ctggtgattg
    28981 gcggcgcgtt tggcattgtg atgcgtacag gaaccattga taacggtatc ctggcgctta
    29041 ttcgccatac ccgcgggaat gaaattctct ttattcctgc gctgtttatt ctgttttcac
    29101 ttggcggcgc ggtatttggt atgggagaag aggccgtcgc ctttgccatt atcatcgcac
    29161 cgctaatggt ccggctgggc tatgacagta ttaccaccgt cctggtgacc tatattgcca
    29221 cgcaaatcgg ttttgccagt tcgtggatga acccgttttg tgtggtcgtt gctcagggga
    29281 ttgccggcgt tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg gttatcgcca
    29341 ctctgattgg cctgatcttt accatggtgt acgcctcacg agtgaaaaag aatcctcttc
    29401 tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa gcaggctgat gttgaacaac
    29461 gtccgtttac ctttggtgac tggctggtat tgattgtcct gaccgccgta atggtctggg
    29521 tgatttgggg cgtgatcgtt aatgcctggt ttattccaga aattgccagc cagttcttca
    29581 ccatgggtct ggtgattggc atcatcggcg ttgttttccg ccttaacggc atgacggtta
    29641 ataccatggc ttcatccttt accgaagggg cgcgaatgat gatcgcccct gccctgctgg
    29701 tgggtttcgc caaagggatt ttgctgctgg tcggtaatgg tgaagcaggt gatgccagcg
    29761 tgttaaatac catcctcaac agcattgcca atgccattag cggtctggac aacgctgtcg
    29821 cggcctggtt tatgttgctc ttccaggcgg tatttaattt ctttgtgaca tccggttctg
    29881 gtcaggcggc gttaaccatg ccgttactgg caccgcttgg cgatctggtc ggtgttaacc
    29941 gtcaggttac cgtgctggca ttccagtttg gtgatggctt cagccacatc atttacccaa
    30001 cctcagcttc gttaatggcg acgctcggtg tttgccgggt ggacttccgt aactggctga
    30061 aggtgggcgc gacactgctt ggactgctgt ttattatgtc cagcgtcgtg gtgatcggcg
    30121 ctcagttgat gggctaccac taaaaatgtt aagagccgca attgcggctc ttttttcatt
    30181 ctgccgtttc agtctctacg gcttcatttt tggcattgcg cttcatccac aacgccagcg
    30241 ctttcagcga gtctggagtg aactcatcgc agcgtgcggt gatttcttcc ggcgtcagcc
    30301 agcaaacttc actgacttca tcttcctgta gggcgaaggg accgtgagag acgcagctga
    30361 acaatgcgcc ccagacacgg caatttttat cttcgaaata gaactgcccg tgctcagcaa
    30421 aggggacacc ggcaatgccc aactcttctt ccgcttcgcg acgcgcggat tccagcagtt
    30481 gctcatcggc ctggaccaca ccgcctgcgg tcgcatctaa catgccgggt aaaaagtctt
    30541 ttgtctcggt acgacgctgg accagaattt tgcccatgcc atcatgcacg acgatgtaag
    30601 ttgcacgatg acgcagacac tgtgcccgca tttgttcccg gctggcttgt gcaatgactt
    30661 cgttctcttc attgacaata tccacccatt cagtacttgc caaacgacgc tgttccatta
    30721 tcaggaaacc ttctttttct ggcgctctta cggcgcattt tgagttgtgg gtaaattacg
    30781 gattaatcgc gacctgcgca atgatacttt gatcattgag tgcgattaca ctaagtacgt
    30841 cattatccag catgccataa ctcgccggat taccgccttt cggaatactc accgagccgg
    30901 ggttgaagtg gaaaatctcc ccccgctgtt ctgccactgg cagatgggta tggccgtaca
    30961 ccagcacatc gttctggttt aaagcaggca gattttccgg gccaaaaaga tgaccatgcg
    31021 tcaaaaacag acgttgtttt tccagtaata cctgttgcca cggcgcggtt atcgggaaat
    31081 gcagcagcat ttgatccact tcgctgtcgc agttgccgcg cacagcgata accttatgtg
    31141 ccacttcatt aagccgttca gcgactttgg ctggcgcgta accctccggt aaagcattac
    31201 gcgggccgtg attcaacacg tcgccaagga tcaccagcca ctgggcaccg ctttgggcaa
    31261 acaactccag aacacgttcc gtcgccggta acgacccatg aatgtccgat gcaaacatca
    31321 gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg atactggatt ctgtcgttgc
    31381 tatcagccag attgcttcgc cgaaagtgca ataaaacgct ggacagacgc tcgctgccac
    31441 tggaatgtcg cataatccac caggcgttct ggcacctcat cgccatgtag taccaggcgg
    31501 ttaatcatta gcgccagatc agtatcagca atgcaccatt caccaaataa attcggctga
    31561 cccagtacta acaaatgttc tgccatcgca aacagtttct ctgcactggc ttttccctcg
    31621 gccgttagtg gcgctttttt cgcccccgca aagacaacat ccgtcggacg ctcttcgcgg
    31681 atgggcatca gatcgctgcg cagccaggcc tgaatctgtc gtgcacgcgc acgattttct
    31741 aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc cagatactcc
    31801 gcaatggcag aagattcact caactcaaaa tcatcgattt gtaataacgg cacacggcgt
    31861 gtctgaccgt aaccttgcca cgtcggctgc aaatgttcac cgctgtcgag gtcgatggtc
    31921 ttgatatgaa acgacaggcc tttttcctgc aacgccaccc aggcggataa cacataaggg
    31981 gagaaaaagt gggcatctga ccaaagcgtg atagcgggtt tactcattat gtcctcggca
    32041 ataaaggggg ctggctatca aactatagag cctttttgtc actgtcacgt gacgaatact
    32101 cacgtaagtc gccgcgcttt ctatacttgt ggtgttcaga tgattaaaca accggagctg
    32161 caatgatcga tctctatttc gccccgacac ccaatggtca caaaattacg ctgtttctcg
    32221 aagaagcaga gcttgattat cgcttgatta aggtagacct ggggaaaggc ggtcagtttc
    32281 gtccggaatt tttgcgcatt tcgcccaaca acaaaattcc ggcaattgtt gatcattctc
    32341 ctgccgatgg cggcgaaccg ttaagcctct ttgagtctgg tgccattttg ttgtatctgg
    32401 ctgagaaaac aggactcttt ttgagtcatg aaacgcgtga gcgcgccgcc acattacagt
    32461 ggttattctg gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat cattttaatc
    32521 acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa actcagcgtc
    32581 tttaccatgt actgaacaag cggctggaaa actcgccctg gctgggaggc gagaactaca
    32641 gcattgcgga tattgcctgc tggccgtggg ttaatgcctg gactcgccag cgaattgacc
    32701 tcgcaatgta tccggcagtc aagaactggc atgagcggat ccgttcgcgc cctgccaccg
    32761 ggcaggcact gctaaaagca caactcggtg atgagcgttc ggatagttaa cagaaacagg
    32821 ttctcgtgta ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg cacaacctgc
    32881 cgctattatt cgtataaaga accttcgttt gcgtacgttt atcggaatta aggaagaaga
    32941 aattaacaac cgtcaggata ttgttatcaa tgtgacgatc cactaccccg ccgataaagc
    33001 gcgcactagc gaagatatca acgatgcgct gaattatcgc accgtaacga aaaacattat
    33061 tcagcatgta gagaataacc gtttctcttt gctggaaaaa ttaactcagg atgtgctcga
    33121 tatcgcacgt gaacatcact gggtgacgta tgctgaagtg gagatcgata aactgcacgc
    33181 gctgcgctac gccgattcgg tatccatgac cttaagctgg cagcgttaac cgccatatcg
    33241 ggaggctgca tgaacatagt gatcaccgga gggacgggat taattggtcg ccatttgatt
    33301 ccgcgtttgc tggagctggg ccatcaaatc acggtagtga cgcgtaaccc acagaaagcc
    33361 agttccgttc tcggccctcg ggtgacgcta tggcaagggc tagccgatca aagcaacctc
    33421 aacggcgttg atgcggtaat caacctggcc ggagaaccga ttgctgataa acgctggact
    33481 cacgagcaaa aagagcgtct ctgccaaagc cgctggaata tcacgcaaaa actggtcgat
    33541 ttgattaatg ccagcgacac gccgccgtcg gtactcattt ccggctcggc aacgggctat
    33601 tatggcgact taggtgaagt ggtggttacc gaagaggaac cgccgcataa cgaatttact
    33661 cataaactct gcgcccgctg ggaagagatt gcctgccggg cgcaaagtga caaaacgcga
    33721 gtgtgcctgc tgcgaaccgg cgtggtactg gcaccggacg gcggtattct cggtaaaatg
    33781 ctgccaccgt ttcgtcttgg cctgggcggg ccgattggtt ccggtcggca gtatctggcc
    33841 tggattcata ttgatgatat ggtcaacggc attctctggc tgctggataa cgacctgcgc
    33901 gggccattta atatggtttc gccctacccg gtacgcaatg aacaatttgc ccatgcgctc
    33961 ggtcaagcgc tgcatcgccc ggccattttg cgcgtccccg caaccgccat tcggctgtta
    34021 atgggcgaat cttcaatact ggtattaggt ggacaacgcg cgctgcctaa acggctggaa
    34081 gaagcgggtt ttgcgtttcg ctggtacgat ttagaagagg cgctggcgga tgtcgttcgc
    34141 tgatgtggtt tacagcaaac atccgccagt taactcccgg tgttacagga ttagtggctt
    34201 tgcgcgataa gatcgtctgg tgaaagtcgg gtcaccatca taactaactc tctgtctaaa
    34261 cctctatcca gcatctcctg agcaatacgc agggcttctt cgtgtttgcc ctgcattgcg
    34321 ccttcttcac gtaatctgtc agcaatggtc atcagtttct ccttttcttg tggtgcgcgt
    34381 tctgttatct caccaataaa tgcacggaaa cgctgggcat cccctgtttg taatacgtaa
    34441 ttaaacaggg cttttagctg tctgtcatta gtgttccctg taactagcag cgaaacaatt
    34501 tggtcgacta atcccaacag atcgcgctga cgaatatgtt tctgaattaa ctccaacagc
    34561 gccattttgc ggtgttgcat aatctcgtca tccggcacca cggtaatatc caccaacgga
    34621 aacgctgatg aatatatttt gcgggctata gcaggctcgg caaattcatc aagccagcag
    34681 agtgaataag gataaggact tctgcaacca tgataaaaca gcatcgggat caccaatgga
    34741 agctctttat agcccgcatc aagatggttt tgcattgccg caatggaata acgcatcatg
    34801 cgaaaagcca ttaattcttc cggcttactt tggtgctcta tcactacata aatataaccc
    34861 actccctcct gcgttttcac agaccacaag aggtcggaat aatattgccg caggtcgtca
    34921 tcaataaaac tgtttggttc cagtttaagc gtcgttaaat cacacagttt gcgcagcggc
    34981 gcgggaagat gaatatcaat aaaatcccgc gcggtgtctg gatggcgtaa aaaagattta
    35041 aataccgcat catgcggcgt ggaagttgtc gatattgtca tggcgttccg tcacccctca
    35101 aatttaagat gacgcgacaa taaccacgat taacacccaa tgcagcaggc agtttttatc
    35161 tttggacagc gaattcagaa gtatttttag cggactgcaa cttttacatt tcagcggaag
    35221 ctgactcaca aattccccat gaaaatactc cggcagcgtt agccaccgga gaggaaatta
    35281 tttcagcgat cccttaagga actgttgcag acgagggctt tgcgggttgc caaataactg
    35341 ctccggcgcg ccctcttctt ctatttttcc ctgatggagg aaaatgacgt gagtagaaac
    35401 atggcgagca aagcccattt cgtgagtcac taccaccatg gttttcccct cttctgccag
    35461 ttgctgcata atacgtaaca cttcgcctac cagttcagga tcgagcgccg aggtaggttc
    35521 atcaaacagt aaaacttccg gttccatcgc cagcgcccgc gcgatagaaa cacgctgttg
    35581 ctgaccgccg gaaagatgca ccggatattt cccctgcgca cgttcgtcta tcccgacttt
    35641 cgccagatac ttcaccgccc gctcgcgcgc ttcctgcttg ctcaggccca acacctgaat
    35701 cggcgcttcc atgacgtttt ccagcaccgt catatggctc cagagattga agtgctggaa
    35761 taccatcgtc aggcgtgtgc gcagtaagcg cagttgattt ttatcggcga ctttgagttg
    35821 accatctttg tcgcgcacca gattgatcgt ctggccattg accacgatcg acccttcact
    35881 cggtttttcg aggaagttaa tgcagcgcag aaaggtactt ttccccgatc ccgacgatcc
    35941 gatgatgctt attacatctc cggcattcgc ttgcagtgag acccctttca gcacttcatg
    36001 ttcgccgtag cgtttgtgca aatcgataac gtttaattta ttctcggaca tcgtattctc
    36061 agtgcgttga agaaggtttc acatgctgca accagcgttt ttccgctctg cgaaagaggc
    36121 tgatcaggac ataagagatg attaaataga gcaccgcggc aatgccgaag gcggtaaaag
    36181 gttgatacgt ggcggcgtta atatcgcggg ctattttcag cagatccggc acagtggccg
    36241 taaacgccag cgcagtagag tgcagcatca ggatcacttc gttgctgtat gccggtaacg
    36301 caatacgcag cgcagaaggc aaaataatgc aacgatacat tttaaaagtc gagaagccat
    36361 aggctctggc ggcttcaatt tccccatgcg gaaccgaacg gattgcccca gcaaaaatct
    36421 cggtagtgta agcgcaggtg ttaagcgtca gcgccagcac ggtacagttc aggccactgc
    36481 ggaagaaagc gttaaggaat tcggttccct taacaatctc aagcgtgtac atgccggaat
    36541 agaacaccag caactgaaca tacagcggcg taccgcgaaa aatataggta aataaccaga
    36601 ttggaaactg gatgtattta ttactggaga cacgaccaat cgccagaaac agcgccagga
    36661 ctccgcctat cactaccgac aaaataagca gccacagagt gatcgccaca ccagtaaagc
    36721 gataaccgtc ggtccacagc agcggtttcc agtattcatg taagatttcg atcacaggtc
    36781 agccctcttc acacccacgg agtagcggcg ctcaaggaac agcagcacac cattggaaac
    36841 agtggtgaaa accaggtaaa tcacgccaca gacgatggcg aaatagaacg gttcccaggt
    36901 acttttgcct gccagttggg tggctttgac cacatcttcc aggccgagta acgaaaccag
    36961 tgcggtagat ttgaggatca cctgccagtt gttgccaatg cctggcaggg cgtaacgcat
    37021 catcgccgga aacatgatcc gccgaaacac ttgcccacga gtaaaaccga acgccgtcgc
    37081 cgcctctata tgccctttcg gcactgccat aaacgcgcca cggaacgttt cggtaaaata
    37141 agcaccgtag ataaaaccga gagtaataat accagcgacc atcggatcga tatcaatctg
    37201 cccgacgccc atcgcctccg tcaccgtgtt tagtgcaatc tgcaaaccgt agaaaatcag
    37261 cagcatcaac accaagtccg gcacgcctcg aatcagcgtg gtgtaccctt cgaaaatcag
    37321 ccccgaaagc cgattttgcg agagcttacc gccagcgcca attaaaccga tgattacagc
    37381 gagcactaca gaactgatag ccagctccag cgtgacgagc gcaccctgta aaataacacc
    37441 tgaaaaccca tacaacatgc tgcctgtcct gtcgtgtgtg gtgaattacc gcctcttgtc
    37501 tccctccggt tacccgaagg gagcgggctt tctttcaccg atggcgatta accgccataa
    37561 acatcaaaat cgaagtactt tttcgctaat ttctcgtaag taccgtcagc gcgcatttcg
    37621 gcaaaggctt tgttcagtgc ctcgcgcagt tcgttatctt ctttacgcag gcccatgccg
    37681 gtccctacgc caaacagttt ttcatcttta acagacgggc caccgaattt gtaatcttta
    37741 ccgacaggtt gtttgaggaa accttcgctg gcagcgacct catcctggaa cgcggcatca
    37801 atacgtccgg cagtcaggtc agaataaatg ttgtcctgcc cctgatacga gacgatttca
    37861 atgccttttg gtgcccaatg ttcattaccg aacgtctcct gggtggtgcc ctgcaatacg
    37921 cctacccgtt tgcctttcag cgactcgact gtcggctgaa tgtcggaatt tttcgccacc
    37981 accaaacgag aatcggcagc gtacagtttg tcggtgaagg ctatttcttg ctgacgtttt
    38041 tccgtaatgg aaagcgatga catgatggcg tcaatcttct tcgcttttaa ggacgggatt
    38101 aacgcatcca gcggattttc gacaaacgta cattgcgtat tgatgcgttt gcataattcc
    38161 tttgccagat cgatatcgaa gccaaccagt tcgccttgtg aattttttga ttcaaatggc
    38221 gcataggtcg ggtcggtacc gatgcggatg ttttgcggaa tcgcagcaaa cgccgcagtt
    38281 gcgctggaga aggccagaac cagagagagc gatagcacca gttttttcat aatgtcctca
    38341 actgacagtc tttttatagg gaatatttac aggtttgtta cacttatcgt gccataaaat
    38401 tgaccatcaa ggcaaaatat cccgccccgt tagacgtatt atttttcatt aaaaatgctt
    38461 aagtatgcat ttcaatgcac cacgatagtg caacaaacct ggcgtgcacc atgatgatca
    38521 ttccatcagg tacagcttcc cagcgacgta tcagtcaccg tagacattaa agtcgaaata
    38581 ctttttcgcc atcttgtcgt aggtgccgtc ctgacgcagc tcgccaagcg ccttattgaa
    38641 ggcagccgtc agttcagcat catctttacg tagccctaca ccggtgccat caccgaagta
    38701 ttttttgtct tttactgatg agccagcaaa ggcgaaatct ttaccagcag gttgcttgag
    38761 gaatccttcg ctggcagcaa cttcatcttg taacgcagca tccagacgtc ctgcagccag
    38821 atcggaatag accaaatcct ggttggcata ggccaccaca tccacgcctt tgctacgcca
    38881 ggtctcgtta gcgtaagctt cctgggttga tccctgcagc acaccaacat gtttaccttt
    38941 cagtgaatcc agcgttggct gaatcggtga acctttggca gcaatcaaac gagaatctgc
    39001 ggcgtacagc ttgtcggaga aggcaatctc ctgctgacgt ttatcggtaa tggaaagcga
    39061 cgaaataatg gcgtcgattt ttttcgcttt cagtgagggg atcagcgcgt caaagtcact
    39121 ggcaacccag gtacatttca cctgcatccg tttgcacatc tcgttaccga gatcgatatc
    39181 aaagccaaca aaatcacctt tagcatcttt cgacgagaac ggcgcatagg tggtatcggt
    39241 tccgatacgt accgtctccg gtagcgccgc atagctggaa gccgctgcgg agagaccgac
    39301 taacaaagac agagcgagaa tcgacttctt catacataac cctcaagtga aaatggcttt
    39361 tttatgttgt gtattgtgtt gtgtgtttgc aggctctttc atgcaggtct tatgccatct
    39421 tgacgacaac agtaacattc aacgttaaat atgttaataa gacgttgcat tattgtcctg
    39481 aagttgaaga tagcaggtat agcggttgaa tcgcagcgtt tcgattgaat ggcagaaaca
    39541 aaatgtcgag gatttgatcg cggttgcaaa attgccctga aacagggcaa cagcggagtt
    39601 atgcgccctg ccagcgggca aagagatctt caggaagggt tattgcaaac tggtcaagaa
    39661 cacgattaac cgtctgattt atcacatcat caagggattg cgggcgatga taaaacgccg
    39721 gaacgggagg cataatcacc gcaccgattt ctgccgcctg agtcattaaa cgcagatggc
    39781 ctaagtgcaa tggtgtttca cgcacgcaga gcaccaacgg gcgacgctct ttcagcacca
    39841 catctgccgc acgggtcagt aagccatcag tatagctatg gacaatgccg gaaagggttt
    39901 tgattgaaca gggtaaaatc accatcccca gcgtctggaa agaaccggaa gagatgctgg
    39961 cggaaatatc gcgcgcatcg tgcgtgacat cagctaatgc ctgcacttcg cgcagggaaa
    40021 aatccgtttc gagggataag gtctggcgcg ccgcctggct catcaccaga tgcgtttcga
    40081 tatctgtgac atcgcgcaga acctgtaata agcgcacgcc ataaatcgcg ccgctggcac
    40141 cgctgatgcc tacaatgagt cgtttcataa atagttgccc tgtcagactt gcaggcagac
    40201 tttgcagtat ttcggcggga gttgcaagtc agggtgccag accggcaccc tcagcgaagg
    40261 catcatcctt cgttatgcat ttcgagattt tccacttcgt tctgacgttg cactgctttg
    40321 gcgtcatcat tacgtaacgt atcgaggaaa tcgaggtagc cctgatcaac atctttggtg
    40381 acgtagacgc cgttgaacac cgagcattca aactgctgga tatccggatt ttcagcgcga
    40441 acggcgtcga tcagatcgtt cagatcctgg aaaatcaacc cgtcagcacc gatgatctgg
    40501 cgaatttcat caacttcgcg accgtgagcg atcagttccg tggcgctcgg catatcaata
    40561 ccataaacgt tcgggaagcg aatttccggt gccgcagaag cgaggtacac tttcttcgct
    40621 ccggcttcgc gtgccatctc gataatctgc tcagaagtgg tgccacggac gatggagtcg
    40681 tcgaccagca ggacgttttt atcgcggaac tcggcgcggt tggcattcag tttacggcgc
    40741 acggacttac gacgcagctg ctggcccggc atgataaagg tgcggccaac atagcggttt
    40801 ttaacgaagc cctggcggta cggtttgccc agaatacgag caatttccag cgcgatatca
    40861 cacgaggttt ctgggatcgg gatcaccacg tcgatatcca gatcttccca ttcgcgggca
    40921 attttctcgc ccagtttcgt gcccatattc acacgcgcgc tgtaaacgga aattttgtcg
    40981 atgaacgagt ccgggcgagc aaagtataca tactcaaaca ggcacggatt gctgaccgga
    41041 ttgtcagcac attgacgggt aaacaactgc ccttcttcag tgatgtaaat cgcttcgccc
    41101 ggcgcgacgt cacgcaggaa atcaaagccc agcgtatcga gcgctacgct ttcggaagcg
    41161 accatatatt ctgtacggtt ctcgtcaata tcacgttttc ccagtaccag cggacgaatc
    41221 ccgtttggat cgcggaaagc aaccataccg tggccgataa tcatcgccac acaggcatat
    41281 gcgccgcgga ttaagcggtt tgtggcagca atggcagcga aaatattgtc ggcttccagc
    41341 gggtagtggc ggaagttgtc cagctcgcta gcgaagatat taagcagaat ttccgagtcg
    41401 gaagtggtgt tgatgtggcg gcgtttttct tcaaacagtt ttttacgcaa ctcgtgagcg
    41461 ttggtcagat tgccgttgtg ggcaagcgta atgccatacg gagagttaac gtaaaacggc
    41521 tgcgcttcag aggcgctgga gctgccagcc gtagggtaac gcacatgacc aatgcccata
    41581 ttgccctgca aacgctgcat atggcgagct tcaaatacat cgctcaccag cccgttcgct
    41641 ttacgcaaac ggaagcagtt attggcatct atggtgatga tgccggcggc atcctgaccg
    41701 cgatgctgaa gcaccgttaa ggcatcataa atcgactggt taaccggcat aacaccggcg
    41761 ataccgacaa taccgcacat acgtcttttt cctcgttaag ccacatctca gagcacttac
    41821 gctctgggca agaaacttga cgagctttgc agataatcaa aaaaccatct gatgataaaa
    41881 ctgaattgcg ggatcagctg tgatttgctc cagtcttcgc ttttcgacac cccggtaaag
    41941 gagtcgagaa agaagagaat ggcggcaaca atcaacacac cgcgcaacgc accgaaacag
    42001 acgcccagca cccgatcggt gcctgacaac cccgttttct ccaccaactg gcctatcacg
    42061 aagttcacga tagcaccaac gatcagggta gcgataaaca gtaccgcgat ggcaattcca
    42121 tttcgaacca gttcgtcttc aaagcccgta aaccagactg acaggtaagt gtagtaatga
    42181 ctggcaacaa agaaagcaca accccatgtc accagcgata acgcttcacg aacaaagccg
    42241 cggatcaggc taaccagaga ggaaaaagca atcaccgcga ttatggcgta atcaatccag
    42301 accatatgtg tcccacgatt ttacgccctg tcatcctgtt cggggcgcat tctaacagaa
    42361 aaagaaaacg tttgcgtagg gatttccttc ccgcgcatca ataaaaatgg cgctgaaaaa
    42421 atattcaacg ccatcgactt tttatgcctt tgcggcatcg tgcaatgcgt gtcggatgcg
    42481 gcgtaaacgc cttatccgac ctacggttct aacactgccc aggcctgata agacgcgcca
    42541 gcgtcgcatc aggcaagact gtattaattc ggcgtatagc ccattaccac gccacttaag
    42601 ccagaaagtt gcttcaactc acccagcgaa cctttcagct tatctttcga ggcatccggc
    42661 ccaaccagaa tacgggtaat tttaccctgc actggcgtgg atggcgacgt ataaacccga
    42721 taaccggcac cgcgcagctt accgacaatc tcattcactt tatcggcatt tttcagcgca
    42781 cccagttgca caacataggc tttacccgtt ggcgctgctt tttcttccac gaccggcttc
    42841 ggttctggct tcggcgcagg tggtgcttca accttcggtt taggcggctc caccggtttc
    42901 gacttcggtg gggcgaccgg tgcaggttcc ggttcaaact cggtgttatt ggctgcaata
    42961 gtggtcggat cgagcgacgg tgctgcggca tcacctgccc gcacctcttc cgctgcgcct
    43021 tccggcggct gcgtcggcaa cgcctgggtg gcggctggca tcatatcagg ctcatcgcga
    43081 tcgcccgctt tcggcaccag cgggatagcc gcgaactcat cctgataatg ttttttctgc
    43141 ccgtccagca gccctggaag tacaatcacc cccagcgcca ccagcacgat cgtgcccact
    43201 aaccgattct gaaacttact tgccaccgct tctcctcgcg tcaatcactt ccatgacatg
    43261 tgcgacagtg tggaaagaac cacacaccag cacggtgtct tccgctttag cgtccgccat
    43321 tgcggcatcc catgcctgcg caacgctatc aaatgatttg ccgttaccca aatgctcaag
    43381 cagttgttct gccgtggcac cgcgcggccc ttccagtggc gcacaatacc agtcatcaac
    43441 cacgcttttc aaccaggcca gagttccggc aatatcttta tcatgtagca taccgataac
    43501 cgccagcacg cgcccgtttt tcggtagcgc tttcatacgc ccggtgagat attccgccgc
    43561 atgtggatta tgcgcgacat caaaaataac gcgtggcgac tcgctcacaa tctggaaacg
    43621 tcccggcaaa attgcgctgg caatcccgtc gcgaatggca ttttcactga cttccagccc
    43681 gctggcacgc agtgccgcca gcgccgttgc ggcattcggt tgcgggacaa gcggcaacgg
    43741 cagattttcc agcgtgccgt gagcatcgct aaacgcccag tcatgatcgg tgacggaata
    43801 gttccactca acgccccgac gttgtaacag tgcacctttt tcctgtgcca catcagcaat
    43861 ggtagaaggc atttccggct caccgacaat tgccggtttt tcgctgcgga agatgcctgc
    43921 tttctcgcga ccaatacttt cgcgatctgg acccagccag tcggtatgat ccagcgcaat
    43981 actggttact accgcgacat cggcatcgac aatattggtt gcgtccagac gaccgcccag
    44041 ccctacttcc agaatcacca cgtcaagttg tgcctgcttg aacagccaca acgccgacag
    44101 cgtaccgtac tcgaaatagg tcagggaaat atcaccgcgt gccgattcaa tctccgcaaa
    44161 agaggcggtg tgggccgatt ccggcaattc ctggccctgc acacgtacgc gctcggtata
    44221 acgcaccaga tgcggtgaac tgtagacgcc cactttgtac cctgccgcca tcagaatcga
    44281 ctccagcgta cggcaggtgg tgcctttgcc attcgtaccc gcaacggtaa acacaaatgg
    44341 cgctggtttc agaacgccaa gacgcgccgc gacctggctc acgcgctcaa ggccgagatc
    44401 gatagtttta ctgtgcaggt tttccagata agaaagccac gaagccagag gcgacgcggc
    44461 ttgaggagtg cgtttgataa tcatggtatc cgctgattcg ttacggtgag aatagcaaaa
    44521 gggcagagcc agtggccctg cccttatcag ttatcaggcc tcaggttcct gatccggtac
    44581 cgggggtacc actacgcctt cacgcggcgc ttcaggattc ggcgctggca gattcatcaa
    44641 cttcgccaga atgctcgcca gtttcaggcg catttccgga cgacggacga tcatgtcgat
    44701 cgcgcctttc tcgatcagga attcactgcg ctggaatcct ggcggcagtt tttcgcgaac
    44761 ggtctgttcg ataacacgcg gaccggcaaa gccgattaac gctttcggtt cagcgatgtt
    44821 gagatcgccc agcatggcga aacttgcaga aacaccgccc atcgtcgggt cggtcagcac
    44881 ggagatgtac ggcaagccgc gctcctgcat ttttgccagt gccgcagagg ttttcgccat
    44941 ctgcatcagc gacatcagtg cttcctgcat acgtgcgcca ccagaggcgg agaagcagat
    45001 cagcgggcag ttatcttcca gcgcctgctc aacggcacgc acgaaacgtg cacccacaac
    45061 agaccccatt gaaccgccca taaaggcgaa ctcgaatgcc gcagcgacaa ccggcattcc
    45121 atacagagtg cctttcatca ccaccagcgc atctttttcg ccagtttctt tctgcgcaga
    45181 tgccagacgg tctttatact tcttggagtc acgaaacttc agcacatctt tcggctcaag
    45241 ctcgctaccc agctccacaa ggcttccttc atctaacagg ctatgcaggc gattacgcgc
    45301 tgtcatacgc atgtgatggt cacacttcgg acagacctca agattacgtt ccagctcagc
    45361 gcggtataaa acctgaccgc agctatcaca cttagtccac accccttcag gaatgctcgc
    45421 cttgcgggtg ggagtaatgt tgcttttaat tcgttcaatc cagctcatta gggacctttc
    45481 tgtctgaacc tggttcgatg ccagttttat ctttggggac gcataatgcc atttttgccc
    45541 ccaacagacc atgaatgttg cacattaaaa cataacagcc cgaaactttg gataaaaaag
    45601 tggtcgaacc gcggagttac tttttatttt gcggcgcgtg ccgcagcgcg tttgtgacga
    45661 ataaattcga aaaccccagg caatattgaa acaaaaataa tgccaacaat catcagctta
    45721 aggttgctct gaatgaaggg gagcgtgccg aagaaataac ccgcgtaggt aaaaatcagt
    45781 acccacaaca gtgcgccgat cacgttataa gcggcgaaat gacggtacga catgtggccc
    45841 attcccgcaa caaacggcgc gaacgttctg acgatcggta caaaacgggc gagaataatc
    45901 gttttgccgc catgtctctc gtaaaactga tgagtcttat caagataact gcgacgaaag
    45961 attttcgaat ccgggttact gaacagtttg tcaccaaata accgtccaat ggtatagttc
    46021 actgcatcac cgacaatagc ggcaatcaac ataagcacca ccatcatatg gacattgaga
    46081 tcgttagttt ccagcgacgc caatgcacca gcaacaaaca gcagcgaatc accaggcagg
    46141 aatggcgtta ccaccagccc cgtttcgcag aacagaatca aaaacagaat ggcataaacc
    46201 cagacgccgt actccgcgac cagctctgcc agatgcacgt caatgtgcag gataaaatcg
    46261 atgaggaaat aaatcaggtc catatttgcc tatgccttgt actcgtcatc tttcaggctg
    46321 taactacgtt ggctacagcc tgaaatgttc cgagcattat tctcgttagt ccgccagaaa
    46381 tagcgggccc attggcggtt ttggaagatc ataccggtca gggtaatcca ccgcgaccag
    46441 atacagccct tccgcttttg ccgttgctgc cgcccgcgtt ctgtcctttg ccgccagcaa
    46501 ctctgctatc cagctctccg gctggttgtg ggcaccgact tccatcaggc tgccgacaat
    46561 attcctgacc atatgatgta caaaggcatt cgctttgata tctaccacca cataaggacc
    46621 gtgacgcatg acattaatat gcataacgtt gcgccacggg gttcgggact ggcactgcac
    46681 cgcacggaac gaggtgaaat cattctcgcc cagcaagcat tgcgcagccc gatgcatccg
    46741 ttcagcgtcc agcggttcgt aaaaatgggt taccccttta ctcagtaccg ccgggcgcag
    46801 ccgatgattg tagatgatgt agcgataacg gcgagccgtg gcgctaaatc gggcatgaaa
    46861 atcatcaggt acagctttaa cccaacgcac ggcgatgtca ccaggtaaat tcgcatttac
    46921 gcccaaggtc cacgccgcgt ctttgcgctg cgcggttgtt tcgaaatgca ccacttgccc
    46981 ggtaccgtgt acccctgcgt cagtacgccc ggcgcagaag acggtgatgg gttcgttcgc
    47041 cacctgggag agcgcctttt ccagcttctc ctgcacactg cggacttcat tctgccgttg
    47101 ccagccgtaa tacttactgc cgtcgtactc aatgcccagc gcaattttat aaactggcag
    47161 ttgttgctgg tcggacatta gtacagatac tcctgcacca gtttctcggc gatttttact
    47221 gccatcagcg cgccgccaaa gcgaacgtta tcggccaccg accagaactg gacttgctcc
    47281 ggcataccgt agtcattacg cacgcagcca acagaaagat gcggcgtacc cgaagcatcc
    47341 cctacctgag tcgggaattc gttctcttca gagagcacaa tatcttcgcc ctgagcaaac
    47401 gcatcacgcg cttcttctgc cgccagcgga cgcagtgctt caaagttgac catctgggca
    47461 tgaccgtaga ataccggtgc ctggacgacg cttgccgaaa tcatcagccc ttcgtcctgc
    47521 aggattttgc gtacttcgtc aacgatacga cgttcttcac gcacgctacc ttcgctatcc
    47581 ggcaacaacg gcagcatatt gaacgccagt tgacgaccga agaaatcttc ttcgtcaatc
    47641 ggaatgccgt tgagcaattt cgcactctgc cccgctaacg catcgaccgc ttttttgccc
    47701 tgggcggagg ctgaaatcag gctggtaaca ctgatacgcg acaaaccgcc ctgatcgatt
    47761 aacggtttca gtgccgccag cagctggctg gtcagactgt ctggtacggc gatgacattc
    47821 cggttgcggt aatcggtcag tacaaacggg tttacttccg gcaccaccag cggtacgtcg
    47881 ggttcgagag caaacagtcc gctgctgtcg atcaccaggc aacctgagtt ggtcgcttct
    47941 tcaacccagg cagcggtagc ttctttgcct gcgacaaaaa atgccagctg cgcctgcgtc
    48001 cagtcgaatt cagcggcatc ctgcacggtg attgttttac caccaaagcg cagttgttcg
    48061 cctgcgcttt cgttacgtgc cagtgcataa atttccccaa ccgggaactg acgttcagcc
    48121 agcgtttcaa gcagggcttc gcccacagcg ccagttgcgc ccaggacggc aatgttccag
    48181 ccttcagaca tggtggttta ctccagaaat agcaaagctc cctgccaatg ttacagcaga
    48241 gagcatgaag aagagattaa cgtgccggat gatgaacggc gttaaaaccc agtttacaca
    48301 gcaatgatgc cgcactggcg tcatcacaaa ttacatacag agatgaccat tcacggcgct
    48361 caagatagtt tttgcgcagt ttatcgaact cacccggtat cccggcgact ttacgcagcg
    48421 gtgcgtcatc gcggcgcaca tcatacacca aatgcaccag ccttttcagc gtcggttgat
    48481 cgagcgggcc atgcagcgta atgcgaccaa actctggcgc gggcagtaat gtatccagcg
    48541 caacgtgctg ttcatgccca ataaacttgc tataggcttc aaacacttgc gtagtaccgc
    48601 gtgctttacc ttccagggta taacctgcga tatgcggcgt gccgatatcc acttttgtca
    48661 gcaactccac gttaagttcc ggttcgcctt cccagacatc cagcactacg cttaacttct
    48721 ggccttcatt caggcaggtc agcaacgcag tattatcgac gactgcgcca cggcaggcgt
    48781 taatcagaat cgctcccggc ttcaggctac ggatcagttt ttcatccgcc agatgcagcg
    48841 ttttgtacgg cccatctttg aagagtggcg tatggaaagt cagaatatcc gcatgctgaa
    48901 ctaattcatc cagcgagcgg aaatcaccct catccccacg gtcagcgcga ggcggatcgc
    48961 aaagcaatgt tttaatccct aacgcttcca gtcgcgcctg caatcgacgt ccaacgttac
    49021 caacgcccac gatccccacc gtacggtcgt gcagtgaaaa tccatcgcgt tcggcaagca
    49081 tcagtagtga ggagaaaaca tattccacca ccgcaatcgc attacagcca ggtgcagcgg
    49141 aaaaaccaat tcccgcctgc ttcaaccatg cttcatcgac atggtcggtc cccgcagtgg
    49201 cagtgccaac aaatttaatg ggttttcctg ccagcaaaga ttcattcact ttcgtgaccg
    49261 aacgcaccat cagcgcatcc gcgtctgcca gttgagcaac ggggattgga cgcccgggaa
    49321 ccgcggtcac ctcacccaaa cggctaaata agtcgcgggc ataaggcata ttttcatcaa
    49381 caaggatttt cacgtttgtg ttacctgtat gagacgagag ttaaccggac aagtgtgcca
    49441 taatctcgcg gccaggcata cttgcgaaga tttcaggtat aaggatacgt aatgatacaa
    49501 cctatttccg gccctcctcc tgggcaacca ccaggtcagg gagataacct gccgtctggc
    49561 gcgggcaatc agcctttatc cagtcagcaa cgtacttcgc tggaaagctt aatgacgaaa
    49621 gtgacctcac tgacgcaaca acaaagagca gaactgtggg cgggtatcag gcacgatatt
    49681 ggtctgtcgg gagattcacc gctgctttcg cgtcacttcc ctgccgctga gcataatctg
    49741 gcgcaacgtc tgctggccgc gcaaaaaagc cattctgccc gccagctttt agcgcaatta
    49801 ggggagtatt tacgtctggg gaataatcgt caggcggtca cggattatat ccgtcataac
    49861 tttggtcaga cgccgctgaa tcagctctca ccggagcaat taaaaaccat tctcaccctg
    49921 ttgcaggaag ggaagatggt tattccgcaa ccacagcagc gcgaggcgac cgaccgtcct
    49981 ttattaccgg cggagcacaa tgcgctaaaa cagctggtga ccaaacttgc ggcggcaacg
    50041 ggggaaccca gcaaacagat ctggcaatcg atgctggaac tttccggggt gaaagatggc
    50101 gagttaattc cagcgaaact gtttaaccat ctggtgacct ggttgcaggc gcgccagacg
    50161 ttaagccagc aaaatacgcc gacgctggaa tcactacaga tggcgctaaa acaaccttta
    50221 gatgccagtg aactggcggc gttatcggca tatatccagc aaaaatatgg cctttctgcg
    50281 caatcatcgc tttcttctgc ccaggccgag gatattctta atcagcttta tcaacggcgg
    50341 gttaaaggga ttgatccgcg tgatatgcaa ccgctgctta atccttttcc accgatgatg
    50401 gacacgttgc aaaatatggc aacgcgtccc gcgctgtgga tactgttagt cgcgattatc
    50461 ctgatgctgg tctggctggt tcgttagccc cgacgaaatg acagtatcgt gacaataatt
    50521 cccagcaccg cagagatcgc cccggcaaga aataccgaag agtaaccaaa cgtggtcgcc
    50581 agcattcccg ccagcggccc ggagacgccg agggcgatat cctgaaacgc ggcgtaaccg
    50641 cccagtgcgg tgccgcgaac ttgtgagggg acgcgtttaa ccacctccac gcccagcgca
    50701 ggaaagataa gcgaacatcc ggctccggtt aacgccgcgc ccgctaatgc gacccacgca
    50761 cctggggctt gccagagcag caacaagccc accgtttcta caagcagaga gacaatcgcc
    50821 actttcacgc cgccaaaacg gtccggcatc cagccaaaca tgacgcgcat cacgacaaat
    50881 gcgccgccaa acgcggtaag agtaaagccc gccatcgccc atcctttgct ggcaaagtag
    50941 agcgaaacga aagtcccgat aaccgcaaaa ccaacgcctt gtagtgccag ccctaaccct
    51001 ggtttccaga taaacccgac aacgctccac agcgatggac gttctcccgc cagggccggt
    51061 actttgcgca ctgtgccgtt acaggcccac gccagtaagg gtaataccat tgtggtgatc
    51121 gccagtgcgg caaaaccgta atggctatga atcaacaggc caagcggagc accaacagcg
    51181 agggcaccgt aaatcgccat tccattccat gacatcactt tgccagagtg ttttggccct
    51241 acgatgccta acccccaggt cagagcgcct gtcagtaact ggctttcacc aaacccaaga
    51301 atcaaacgcc cgatgaccaa cagggcaaat ttgaacggtg cggagacagg caaaatcgcc
    51361 gccagcagca acgcgccgcc agccagacca caagctaaca tcccctgaag cgccgaacgt
    51421 tttgcaccat attgatcggc cagtcgcccg gcatagccac gcgtcagcac cgtagccaga
    51481 aactgaatcc cgacggcaat cccgaccatg gtattgccat agcccagatc atgatgaaca
    51541 aacagcggga taaccggcaa cggcagccct acggtcatgt aggtgagaaa aaccgcaaaa
    51601 gcgatgcgga agagcgaaaa attggcagaa gatcgtgttt cggtttggct tacagcagtc
    51661 atgcattact ccagaatgca gcgcaaggcg aggagtatcc ccgtctcatc tctctggttt
    51721 cagggttacg gtgcgttggc aggatttaac gcgtacgtct tttcagaagg aaatcgacaa
    51781 agcgggaagt ttgcctggaa caggcggcga ttgtcaatga tgtgaaaaag ggaaccatca
    51841 ggttcccttt tgcgttagtg ccggaggccg cattagcgcg taacgtcgga tgcgacgctg
    51901 gcgcgtctta tccgacctac tccgactact ccggcctact gcgaattaat ctttcagctt
    51961 gcgcattacc agcgtggcgt tggtgccgcc gaagccgaag ctgttagaca taacggtggt
    52021 cagttcgcga tcggtcgttt cggtcacgat gttcagacct gcagcctgct cgtccagctc
    52081 ttcaatgttg atgctcgggg cgataaagcc gtgttccagc atcagcagag agtagatagc
    52141 ttcctgtacg ccagcagcgc ccagagagtg accggtcatg gctttggttg cagaaatcgc
    52201 cgggctctta tcgccgaaca cttcacggat agctgccagt tctttcacgt cgccaaccgg
    52261 agtcgaagta ccatgagagt tcaggtaatc gattggggta tcaacgccgt gcatcgccat
    52321 cttcatgcag cgtactgcgc cttcgccaga cggagcaacc atgtctgcac catcagaggt
    52381 tgcgccgtag ccaacgattt cagcatagat gtgagcacca cgcgccagcg cgtgttccag
    52441 ctcttcaacc actaccatac cgccgccgcc agcgataacg aaaccgtcac ggtgagcgtc
    52501 gtaagtacgg gaggcttttt ccggggtgtc gttgtattta gtagacagcg cacccatcgc
    52561 gtcgaattcg caggccattt cccagcacag ctcttcgccg ccgccagcaa acacgatgtc
    52621 ctgtttgccc agttggatct gctctactgc gttaccgata cagtgtgcgg aagtcgcaca
    52681 cgcggagctg atggagtagt taacgccatg aattttaaac ggcgtggcga ggcaggcaga
    52741 aacgccggat gccatcgctt tggtgaccac atacgggcca accgctttca ggccgcgcgg
    52801 gccgcgcatt gcgtcagcgc cgaacacctg gaaacgcggg gaaccgccgc cagaacctgc
    52861 aatcaggcca acgcgcgggt tattctggta agcttccgga gaaaggcccg catctgcgat
    52921 tgcctgctcc atagaaagga atgcataaat ggatgcgtcg ctcataaagc gcacaacttt
    52981 gcggtcaatg aggccagtgg tatccagttt tacgttgccc cagacgtggc tacgcatgcc
    53041 ggaatccttc agctcctgag agaaagtgat ccctgaacgt ccttcacgca gagatgccag
    53101 gacttcctgc tggttattac cgatgctgga aacaatgccc aggccagtaa tcactgcacg
    53161 tttcattcaa tacctctgta agtcgcacat agagtaagtt tcgaatgcac aatagcgtac
    53221 acttgtacgc cgaacaagtc cgatcagcca tttaatagag aaatttgcgc agccttacac
    53281 acatcgctaa gatcgagcca ccgcctgtaa gacgagtaac ttacgtgaaa cactactcca
    53341 tacaacctgc caacctcgaa tttaatgctg agggtacacc tgtttcccga gattttgacg
    53401 atgtctattt ttccaacgat aacgggctgg aagagacgcg ttatgttttt ctgggaggca
    53461 accaattaga ggtacgcttt cctgagcatc cacatcctct gtttgtggta gcagagagcg
    53521 gcttcggcac cggattaaac ttcctgacgc tatggcaggc atttaatcag tttcgcgaag
    53581 cgcatccgca agcgcaatta caacgcttac atttcattag ttttgagaaa tttcccctca
    53641 cccgtgcgga tttagcctta gcgcatcaac actggccgga actggctccg tgggcagaac
    53701 aacttcaggc gcagtggcca atgcccttgc ccggttgcca tcgtttattg ctcgatgaag
    53761 gccgcgtgac gctggattta tggtttggcg atattaacga actgaccagc caactggacg
    53821 attcgctaaa tcaaaaagta gatgcctggt ttctggacgg ctttgcgcca gcgaaaaacc
    53881 cggatatgtg gacgcaaaat ctgtttaacg ccatggcaag gttggcgcgt ccgggcggca
    53941 cgctggcgac atttacgtct gccggttttg tccgccgcgg tttgcaggac gccggattca
    54001 cgatgcaaaa acgtaagggc tttgggcgca aacgggaaat gctttgcggg gtgatggaac
    54061 agacattacc gctcccctgc tccgcgccgt ggtttaaccg cacgggcagc agcaaacggg
    54121 aagcggcgat tatcggcggt ggtattgcca gcgcgttgtt gtcgctggcg ctattacggc
    54181 gcggctggca ggtaacgctt tattgcgcgg atgaggcccc cgcactgggt gcttccggca
    54241 atcgccaggg ggcgctgtat ccgttattaa gcaaacacga tgaggcgcta aaccgctttt
    54301 tctctaatgc gtttactttt gctcgtcggt tttacgacca attacccgtt aaatttgatc
    54361 atgactggtg cggcgtcacg cagttaggct gggatgagaa aagccagcat aaaatcgcac
    54421 agatgttgtc aatggattta cccgcagaac tggctgtagc cgttgaggca aatgcggttg
    54481 aacaaattac gggcgttgcg acaaattgca gcggcattac ttatccgcaa ggtggttggc
    54541 tgtgcccagc agaactgacc cgtaatgtgc tggaactggc gcaacagcag ggtttgcaga
    54601 tttattatca atatcagtta cagaatttat cccgtaagga tgactgttgg ttgttgaatt
    54661 ttgcaggaga tcagcaagca acacacagcg tagtggtact ggcgaacggg catcaaatca
    54721 gccgattcag ccaaacgtcg actctcccgg tgtattcggt tgccgggcag gtcagccata
    54781 ttccgacaac gccggaactg gcagagctga agcaggtgct gtgctatgac ggttatctca
    54841 cgccacaaaa tccggcgaat caacatcatt gtattggtgc cagttatcat cgcggcagcg
    54901 aagatacggc gtacagtgag gacgatcagc agcagaatcg ccagcggttg attgattgtt
    54961 tcccgcaggc acagtgggca aaagaggttg atgtcagtga taaagaggcg cgctgcggtg
    55021 tgcgttgtgc cacccgcgat catctgccaa tggtaggcaa tgttcccgat tatgaggcaa
    55081 cactcgtgga atatgcgtcg ttggcggagc agaaagatga ggcggtaagc gcgccggttt
    55141 ttgacgatct ctttatgttt gcggctttag gttctcgcgg tttgtgttct gccccgctgt
    55201 gtgccgagat tctggcggcg cagatgagcg acgaaccgat tccgatggat gccagtacgc
    55261 tggcggcgtt aaacccgaat cggttatggg tgcggaaatt gttgaagggt aaagcggtta
    55321 aggcggggta atctgctctc gcagtgtttg ccggatgcgg cgtgaacgcc ttatccgacc
    55381 tacgtgtgac gtgtaggcct gataagacgc gataagcgtc gcatcaggca tggtgctcca
    55441 gacgccgcaa cttactgctg tgacgcctgc tgaaacaggt tttcccacat atcggtcacc
    55501 agcgcctggt cacgcggcga caattcaccg gcaccaatgg ctttttccag actctggcta
    55561 acggtcgtat gcaccgcctg agcggagtgg tcatcaccac tttccagttc tgcgatggct
    55621 aatgtcaggt ggccacgcaa atacccactg gcaaacaact catcatcact ggcatggtca
    55681 accataccgt cgattaatgc cagaatgcgt gattcaaact ccgcgatcat cttctttcct
    55741 cattgtaaaa cgtggcaccg gcttcagttg agcgcttccg gccacgggaa ctgttccgcc
    55801 gtaagttccg gcgtgtgata ataattttgt agtgctttaa tgaagcgtgc cgggcgttca
    55861 ggaatgccgt ttgctaggta atccatcacc tgcgcatgta cccgccgttg aaacaccaca
    55921 cgatccggtt cgaagtcgcc ttccagattg tcgcaactga cgttaaacgg atatcccgcc
    55981 gccacacaga acaaccagtc gaacgcctgc ggcttcactt caacatcttc aaactggctt
    56041 tgcgtttggg catcgcgtcc gtccgggcaa taccagtagc cgaaatcaac cagctcacgg
    56101 cgcgctttcc cagcaataca ccagtgcgaa atctcatgaa ttgcgctggc ataaaagcca
    56161 tgagcaaaga cgattcggtt atacggtact tccgcatcag caggaagata gatcggttcg
    56221 tcgtcgcctt taatcagacg ggtattaaat tcatcggcaa agcagctatt aaaaatttca
    56281 atcaactgct catagtggtg tgtactgttc attagttcat ccccaaccag tggaggatct
    56341 cctgtccgtg gctatcaaaa agtaatttgg cactcatcac cgctgagaca ataacaatca
    56401 tcgggcggat cagcttttgt cctttgctca acaccagtcg tgaacccatg cgcgcgccga
    56461 ggaactgccc caccagcatg acaaagcccg tcgcccaaat cactttgcca ccgagaataa
    56521 acagcagtaa accgccgatg tttgacgttg cgttgagtaa tttggcgtgc gccgtggctt
    56581 tggcgaggtt gaatccgcac agcgtaacaa aggccagtgc gtaaaacgat ccggctgccg
    56641 gaccaaagaa tccgtcataa aaaccgacgc agccaccggc aattaacgcg aagggtaaac
    56701 cgtacatccg acgctggcga tcttcttcac ccagctttgg catcagcaag aaatagaggc
    56761 cgatacaaat taccagaatg ggcaaaatct gccgtaagac atcagcctga acgtattgca
    56821 ccagcagtgc gccgctcatt gagccgacaa aggtcatggc gatattgagt ttctgatcgc
    56881 ttaaactaac cactttgcgg cgaataaagt agatagtagc ggaaatagag ccgccgcagg
    56941 cttgcagttt attggttgcc agcgcattag cgggagacat ccccgctgcc atcaatgccg
    57001 gaatggtgag taacccaccg ccaccggcaa tcgagtcgat aaatcccgcc agcatggcga
    57061 caaaaaagag aactcccagc aacagcgggg aaaccataaa caggctatta aacgtttcca
    57121 ttagatcacg tgctcatcca gtagcgcctg gcaggaaggc ggcaacggag gcggtgtctt
    57181 cttctcaggc tttgttgttc ccggttttgg aggttcaaac cagctttgca gttctgcccc
    57241 gcaaccatcg cctggtggcg gtaaaggttg atcttcacac tccagactat cggcaggaca
    57301 acgtaatcgt acatgcatat gcgcacgatg ctggaaccag ggtcgcactt tgcgcaacca
    57361 gtcgcgatcg gtgcccgcat ctaggcaaag ttgttgttta atcgccggat taacaaaaat
    57421 gcgcgtgacg tttttgtcct gggcggcgag tttgatcaag ctgaaaattt ctggcttcca
    57481 cagcgaggag acaacgtgtt taccgtcgcg ggaaactaag tccagtgctt gcgggcgcaa
    57541 gagctgcgcg gaggtccagc gagttttcgg cagttgcaga aagatatcga catccagtcc
    57601 ggtctggtgg ctggcatgac cgccgttgaa acgcccacca gcaggcatcc ccatatcgcc
    57661 aatcagcacc gtacccatgc ccagattgct cacctggcta ctcagacgct ggataaacat
    57721 caccagatcc gggtgaccga aatagcgacg ctgatcggta cgcatgacct gataatgttc
    57781 ggactgtatc ggcagcgtgt cagcgccgac aatacagcca ttagaaaaac tgcctatcga
    57841 ttgtgcgcta cccggcacag gttgggttat tttttgccac ggcgtcgctg ccaggctagc
    57901 gctactggca agcagagcca gcagcgcaat cgcggttttg ttcatttttt accagcgtgg
    57961 aatatcagtc ttcacatcgg cattttgcgc ccgctgccgt aacaggtgat ccattaaaac
    58021 gatcgccagc atcgcttctg cgatcggcac tgcacggatc ccgacacagg gatcgtgacg
    58081 gcctttggtg atcatctcaa cttcttcgcc aaagcggtta atagtgcgcc ccggcacggt
    58141 aatgctggag gtcggtttca gcgccatatg ggcaatgatt tgctgcccgc tgctgatacc
    58201 gccgagaatg ccgcccgcat ggttgctctg gaaaccgtct ttggtgattt cgtcgcggtt
    58261 ctggctgccg cgcagcgcca ctacgtcaaa accgtcgcca atttccacgc ctttcaccgc
    58321 gttgatgctc atcagcgcat gggcgatgtc ggcatccagg cggtcaaaga ccggctcgcc
    58381 aagtccggcg gggacaccac tggcaacaac ggtgactttc gcgccgatgg agtcgccctc
    58441 ttttttcagc gcgcgcatca gttcatctaa cgcgtcgatt ttgtccgggt ccgggcagaa
    58501 gaacggattt tgttcaacca gcgaccagtc tttgatttcc agcggaatgt cgcccatctg
    58561 ggtcaagcag ccgcggattt caataccaaa tttctcggcg agatattttt tggcaatcgc
    58621 ccctgccgcc acgcgcatgg cggtttcgcg ggcggaagaa cgtccaccgc cgcgataatc
    58681 gcgcagaccg tatttttgtt cgtaggtgta atcagcatgg cctgggcgga aaacgtcttt
    58741 aattgcgctg taatcctgag agcgctggtc agtgttttcg atcaacaagc caatgctggt
    58801 gccagtagta acgccttcaa aaacaccgga gagaattttg acctgatccg gctcgcggcg
    58861 ctgggtggta tagcgcgatg tcccagggcg acgacggtcg aggtcatgtt gcaggtccgc
    58921 ttccgtcagc ggaatgcctg gcggaacacc atcgacgatg cagccgagcg ccagcccatg
    58981 cgattcgccg aaggtggtta cgcgaaagag ttgtccaatt gtgtttccag ccatcacggc
    59041 tccgttattg ttgtgtttgc gtgtttactt aatctttata aatcgcgaaa tattcacggg
    59101 ccgccagcag ctgctctttg gtgagcataa acacaccatc gccgccgtta tcaaactcca
    59161 gccaggtgaa cggaacatcc ggatattgtt ccataagatg taccatgctg ttgccgactt
    59221 cacaaatcag cacaccatca tcagcaaggt aatctgccgc gttaccgaga atgcgacgcg
    59281 tcagtttcag gccgtcggtg ccagatgcca ggcccagttc cggctcgtga cggtattcgt
    59341 ttggcaggtc ggacatatct tccgcgtcga catacggcgg gttagtgaca atcagatcgt
    59401 attgcacttt cggcaaatca cggaacagat cggaacgaat cggaatgacg ttgtggatca
    59461 gaccgtgttc ttcgatgttc tgttcggcaa ccgccagcgc gtcgggagag atatctaccg
    59521 cgtcgacttc cgcttccggg aaggcataag cacaggcaat ggcgatgcag ccgctaccgg
    59581 tacacatatc taaaatatgc tgcggttgct tgctgatgag tccggcaaat ttattgttga
    59641 tcagttcacc aatcggtgag cgcggcacca gcacgcgttc atcgacgtaa aattcatgac
    59701 cgcagaacca ggctttgttg gtcaggtaag ccaccggaat gcgctcgttg acgcggcgga
    59761 tcacgcgttc aacaatacgg tgtttttcgc tggaggtcag acgcgcggtg cgcatatctt
    59821 ccgggatatc cagcggcagg tagagcgatg gtaacaccag ctgtacggct tcatcccacg
    59881 ggttatcggt gccgtgaccg taccagatat ttgccgcgct gaagcggctt accgaccagc
    59941 gcaacatgtc ctgaatggtt tgcagctcat ttactgcttc atcaacgaaa attttatcca
    60001 cgtattcctc cagggcatga tcgcaataat ttcggcggct agtttgccat gaagatgacg
    60061 ataaatcagc attcacgcgc ggtgagtgag gaaaaatacg tttaaaacga tcgattgtgc
    60121 tacgagtcgg gtaaactgta ggaaaattag aaatagagac agataaatga aaaagaaaac
    60181 aacacttagc gaggaggacc aggctctgtt tcgccagttg atggcgggga ctcgcaagat
    60241 taagcaggac acgattgtcc accgaccgca gcgtaaaaaa atcagcgaag tgccggtgaa
    60301 acgcttgatc caggagcagg ctgatgccag ccattatttc tccgatgagt ttcagccgtt
    60361 attaaatacc gaaggtccgg tgaaatatgt tcgcccggat gtcagccatt ttgaggcgaa
    60421 gaaactgcgc cgcggcgatt attcaccgga attgtttttg gatttacacg gtctgacgca
    60481 gctgcaggcc aagcaggaac tgggcgcgtt gattgccgcc tgtcgccgcg aacatgtgtt
    60541 ttgtgcctgc gtgatgcatg ggcacgggaa gcatattttg aagcaacaaa caccgctgtg
    60601 gctggcacaa catccgcatg tgatggcatt tcaccaggca ccgaaagagt acggcggtga
    60661 tgcggctttg ttggtgttga ttgaagtgga agagtggttg ccgccggagt tgccctgagg
    60721 agttgagcgt gttgtgccag gagcgcggaa tggcaggggc gaatgaccct gccaggtgag
    60781 aaaaggtgcg ctaatacata ttcagtatgt aagtgaatcg gcaggtagaa tctatgggct
    60841 gtcgagatgt cgtgtccacg cgtagcttca tatcatgcag ttgcctggac gtgatcggtt
    60901 gcggctctgg cgtcaccgtc actttggcat tatcacagcc ggaagtcgag aaggtcatct
    60961 gctgtgcgac aaaacggcag taggctccac gctcgtcaaa attactgcca tcacacagtt
    61021 tgccgttttc catcaggaaa gtgggcagtg tttgctcaat aacaccacct gttgactgca
    61081 acctgacgac tttgttatgt acaacataat tttcattcca ggaaacatca tacggtgcca
    61141 gggactctgt cgggcagtca gagctaactt ttgttggtcc tataaaaatt ccggccccga
    61201 aacgctgatc catagtactg gcgctttcaa cataagacga cacaatccag ggacaaccgt
    61261 gtaaatctcc atcgtaataa acgccaagta ctaattcatt acatgaccag gccgttactg
    61321 gagagagagc atcatactct tctaatccgg ggcaggtaga atccggtaag gttgtattat
    61381 tgcgctccat cctgagttca gggccgggag gactcccggt ccaactaact aaaggcaaac
    61441 ttgctgccgc agttgaatta gattcatacg tgccatattc aactgattga ggggtcagga
    61501 ctgaaagatg aaaacgatac tttgcaacaa cgctggtcgt ccccgcgggt acatagagtg
    61561 tggcgatctt tgtcgcagcc tgtatcccag aagcgcacag agccagcacc aataaacata
    61621 taacatgacg tattttatgc attttatata tactcaaacg tagctgtcag gctggcggtg
    61681 aaatcaccga tggttacatc acgaccgctt ttcgcctgga tccaggcccg gaaattaata
    61741 tcattgttgc cgttggtaag cgcaaaagtc gccccggtgg tgttgttaat atccacccgc
    61801 ttgccgtcga gcgtttccat ccctatcccc acgccctgcg cggtggaggt gtcgtcgact
    61861 gccagaaaac ccggctgacc gctgtcttcc gttccggtga gggtgacttt gacgttgtat
    61921 cccgccggcc ctttgcagtc tttcagctta aacacaaccg gcagatgcgc ggactgcccg
    61981 gcgaccatca aatcccggtt gctgacggtg ggaaaatgaa tttccgccag attggcacca
    62041 tccaccacca gcgtgcagga tttactcagc aggttgccgt aaaaatgcag gttgttatcg
    62101 actgcccgtg cccccgtgct ggcggtcagc agcaggcctg ctaacagcgc ataaccgggt
    62161 gaataaatgt gtgctgtttt cattggtaat ccacccgtaa ggttgcgtaa gcagaaaaat
    62221 tcgtgtccgt cagcaccgca tcagccgctt tgaccggtac cgcctccagc tttggcttct
    62281 ggctaaaatc ggtggcgtta atggaaagcg gctcgttgag cttaaaacgc tgaccgtcat
    62341 gctgaagctc aattccgaag cccggtacat ccgtctcaat agcagagtcg ttgtaggagg
    62401 tggcagtccc cgtccaggag agtgtcatat tccactcggg atcgcggaca tccgagtcgc
    62461 agctcagttc gtagtccact ttctgacgac cataattgcc atcaatgctg tcgataataa
    62521 gatcgcgaaa ttgcacttcg atggtttgcc cgccgctgat gctacaggcg ggcggcgaca
    62581 tcagcgtacc gtggaatgtg atgtcatctt ccgccgccag cacagccccg caaccagcgc
    62641 acagtaaaag gagcaggaat tttttcattg gtaatccacc accatggtca tcgtggcgtt
    62701 aaactccgac ggcgtcagtt gcaccccgct ctgttttacc ggcaccgctt tcagctgagg
    62761 ctgagtgttg acattaaaag gcgtccagtt attttcacca actgcaaaca gggagttgtc
    62821 ggcggcgttt tctatccgca cccccaggcc ggcgatatcc gtgctgagaa ccgtttctcc
    62881 gttgatcacg gcggtattgc ctttgagctg catccgtaag tcatcgctga ctttattacc
    62941 gcagttgagg gtgtatttcg catcctgttg gtaattggta ccatcgatcc tgttgattat
    63001 cacattgcca aatttcacag cgcttccctt cacggagcaa ggcggcggtc catcgaccag
    63061 gattcgcagc gtcagatcca cctgtttgat atcactgcca gcaacagcca aaccgctgat
    63121 gccccatccg gccagcacca gcaaccctgc gcccgctaca aacttgtgac tcataagtcc
    63181 ctcccggcat cagcctttgc ggctctgctc ttcatccacc tggcagctgt tgccggtgca
    63241 tatgaagtac agcggcatcc gcgcgccgta gtcgttgatg taagtcagca ccggcacgct
    63301 gtccatcttc gcattcagcg gtgcggaggt tttcggctcc atcatcagcg gtgtgaatcc
    63361 cgccggcggg ttgccgcctt ttttagcgct ggcgttgctg atgatcacgt agtacggcgt
    63421 cgggttattg acggtgaagt cctggccgtt acggatgagc gtcaccttat gctgccacgg
    63481 acttttcatg ctggtgttct ccagcgcctt cggtcgccag aacagtttga tacgcgtctg
    63541 cagcgcaatc tgcagtgtgt tcggtttatt tgatttcggc ggaatttcgc gcacgttgaa
    63601 atagaacacg ctctcgcggt cggccggcag tttgttgata tccggcagcc cctgcacctt
    63661 cacctggccg ttcatcatcg catcgatacg ctgcactggc ggaagcaccg caagcggcga
    63721 gctgattttg ttgccctttg catcctctat ccagctctgc gccagataag gcagtgtggg
    63781 atcgttgttg cgcagcgtga cgctgatgga cttgtcgctt tcgttaaaca ccagccgtgt
    63841 cctgtcgggc gtcaccgaag cgtggctgga gaaactgacc actgtcgcca gcagcgccag
    63901 tgccgggaca acgatttttg cagtgttagt cagattaaac atggatcatt ccttaatcac
    63961 gttactggtt tactgaaatt gaagtcggag gttccgttgg cgtcacccgc tgtgtctgtt
    64021 cctgaatcgc cggtttgacc gccggcgaag tgataggtgc tacattgcct ttatgctggc
    64081 atggcagtaa caagccgtta aacagatcgg caggcagcgg gtccggcagg ttgatatcgc
    64141 aatgcgcaac accactccag aacacactca tgtgttcgcc aggtttcacc ccagccagat
    64201 aaacattgcc gtcatcatcg acaaggccca ctgtctgctc gttatcattt tttacttctg
    64261 cgccaaacgg aggatggctg ccgtcgctca ggcgcagcac cgccatcgct ttttgtccgc
    64321 taatgacagc aaatttacgg tagccaatgg caccttcagt tagcgtggct tgcaccaccg
    64381 actgggttgc ttcagcgttt tcaggcaatc tgttgaggtc gatatacgcc tgattgcggt
    64441 aatagttatt gacgtcagaa acgacggctt taccaaacat attggtataa acagcagccc
    64501 cgttaccttc caccggaaca tcggcaacgc catcggcatc aatcaacaat cgtgtcccgc
    64561 ccatattctg ggtacggtga agtgcgccac cgtgggcagt aagcgttgcg ccgccctgta
    64621 acgacaagcc cgcagaggtg tattgccctt catggtagtt cgcactgagg tcaacctgcg
    64681 ccagcgaacc atcatggctg taatagccgt caacgctggt gtgtttgtca ctggtgccga
    64741 cgttcaactg atagtgagtc gcgtcatcga cacggctgaa ataaccgacc tgactgctgt
    64801 ccgtcccact gccatagtta ccgttatagc taacggtact gttgtcgccc cacggcatac
    64861 tgagcgaaat gtacatgcct ttgtcggcct ggttgtcata ctcgtaacgg tagccagtca
    64921 tcgagatgct gacattacga atactgccca tattgaaata gtgcgagagc atgatgttgt
    64981 agtttgtctg ctcctcgcga tcccagtagg tatggcgggt atagttgaga taaaccgaaa
    65041 cacccacatc gcggaagttc tggttataag tggcggtgta catctctttg tcgttgcccg
    65101 tgcgtaccat tccgctgtca ctggcatcca gatactcgct catggtcata aagttctctt
    65161 ccgagaagcg atatccagca aaagtaacgc ggctgttgag ctggtcaaag tctttggaat
    65221 aactcacacg gaaggagtta ccgtccagcg aacctttgcc ataagcggtg tctttatcca
    65281 gtttggtgtg cgagtgagta acatcaaacg cgaccgcgcc gaatgtagac aaatcgcgac
    65341 cgacgccaag cgccgcagac tgatagtttt catctcccag tgcgccgcca tataacgacc
    65401 agccgttagc gatcccccag gaagcttcag caccagaaaa aaattcaccc tcgatatggt
    65461 gtccccactc ttgcggacgg cccatcatga tcttatagcg aacctgacct gggcgagtga
    65521 ggtatggcat cgaggcggtg ctgatgtcat attcctgcac ctggccgttc tgttcttcaa
    65581 tgcgaatatg caacgtaccg gagacagaat caccaagatc ctgaatacga aacggtccag
    65641 ccggaacctg cgtttcgtaa atcacacgcc ccatctggct gacggtcact tttgctgtgg
    65701 tgtgtgccac gccggaaatg tctggcgcgt agccgcgcag attgggaggc aacatttgat
    65761 cgtcagtact gacactgcca ccaacatagt taaagccatc aaaaatatcg gatctgaggt
    65821 aatcctcgcc cagcgccagt ttggctttta atgatggtaa cgcccgccag gcatagtagc
    65881 gactccactc ccattttttt tgagtttcat cgccgctgaa ttcatcgtca tcatcattac
    65941 tgcgagtatg ttgatagtta gtctgccagt cagcacgcat acgccacggc cccaggttaa
    66001 ccccgaccgt cccgttgccg ctgatctcgt tactatcatc accgccattt tcttcgtgcc
    66061 gggtttgtgc gttgatgctg tagtccgcga cgatcccgga gatgccgtca tcccaacgtg
    66121 aaggcggatc ccagtcggga taagtatatt cgaggtaagc ctgtggcagt gaaatgacta
    66181 atgcggactg gcttaaatca gccttaattt ccatgccttc cagttgaccg gatttcaggc
    66241 atttagcatc gtggctccat tgcagatttt tcgccacgtc ttcttttaaa ccaaactgcg
    66301 ctaccagttc cggtgtcaga caagcatagc ttttgctcgc gtcatcttca ccagcatacc
    66361 agtaaatatc gtactcttcc gccaatggct gtttatttag ttgaacctgt aaattgtatt
    66421 ttccgggctc aacatatccc tggctggaaa aacgcttcag atcaattttt gtgtcgcctt
    66481 ttaattccag aaaacgggaa tcaaactgaa tgtcgtcttc agcccagaca ctgctataac
    66541 tccctgacat tgccagcgca atgcaccacg gaagaatccg taatcgaaaa agagaatggt
    66601 caggcataca cgtttctatc cgtttttatt cttgttcggt caaaatccat ttaaatcccc
    66661 catagcagtg tcgctatggg ggtatgcctt acaggtaagt aatctggaag gtggtatttg
    66721 cttcgaaatt acccagatct ggcgcatcag ctgcgccgac cagccaggct ttaaagttca
    66781 gtgtctgctt cgctttgcct ttattggtag aagtgtcttt cactattttc tggtcaatac
    66841 ccatgccgct tttgtaagag gtacccagag agtcaccgat cgccaggcta acattgttaa
    66901 atgccgcacc ggtatcggta ttgaaaatgg tgtaataatt gccgctattt gcagaagaaa
    66961 cggtaccagt aaaggtagtg gtcattgttt cctgagtatc aaatacgcaa tcttgcagac
    67021 gaatctggaa acttttcggc gtggtggtgc cgttattttt cagtttttcg gcaccgatat
    67081 cacccatatc aacttccagt ttgtgatcgt ccggaacgat agaacacact gagtcttcaa
    67141 tggtgccgta aaaacgtgca gtaccgttgt tacccgcagc gattgttgcc gtagaggtga
    67201 aaacgcccat caccattgcc gcagcaatag ctgttttaac aaacttactc attatttgtc
    67261 cttaaactat aaat
//
LOCUS       DAZGOB010000008        66133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGOB010000008 DAZGOB010000000
VERSION     DAZGOB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.008"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..512)
                     /gene="rfbC"
                     /locus_tag="ACHLIF_000543"
     CDS             complement(<1..512)
                     /gene="rfbC"
                     /locus_tag="ACHLIF_000543"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO9604613.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSEIAEFVYKTTNYYHPESEGCIKWDDSFLMIDWPNK
                     PIAISEKDKKG"
     gene            complement(517..1395)
                     /gene="rfbA"
                     /locus_tag="ACHLIF_000544"
     CDS             complement(517..1395)
                     /gene="rfbA"
                     /locus_tag="ACHLIF_000544"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO9604614.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGLRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(1453..2352)
                     /gene="rfbD"
                     /locus_tag="ACHLIF_000545"
     CDS             complement(1453..2352)
                     /gene="rfbD"
                     /locus_tag="ACHLIF_000545"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO9604615.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWLIHY
                     STDYVFPGNGDMPWRETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQKFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(2352..3437)
                     /gene="rfbB"
                     /locus_tag="ACHLIF_000546"
     CDS             complement(2352..3437)
                     /gene="rfbB"
                     /locus_tag="ACHLIF_000546"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO9604616.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIAQNYEGRQ"
     gene            complement(3809..4702)
                     /gene="galF"
                     /locus_tag="ACHLIF_000547"
     CDS             complement(3809..4702)
                     /gene="galF"
                     /locus_tag="ACHLIF_000547"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO9604617.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSK"
     gene            complement(4877..6271)
                     /gene="wcaM"
                     /locus_tag="ACHLIF_000548"
     CDS             complement(4877..6271)
                     /gene="wcaM"
                     /locus_tag="ACHLIF_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO9604618.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPARQSVNINDYNPRD
                     WIASFKQAFSEGQTVVVPAGLVCLNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNVEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(6282..7502)
                     /gene="wcaL"
                     /locus_tag="ACHLIF_000549"
     CDS             complement(6282..7502)
                     /gene="wcaL"
                     /locus_tag="ACHLIF_000549"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO9604619.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQILRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(7499..8779)
                     /gene="wcaK"
                     /locus_tag="ACHLIF_000550"
     CDS             complement(7499..8779)
                     /gene="wcaK"
                     /locus_tag="ACHLIF_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO9604620.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDSGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKSLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTSKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTIGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQVMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLEHIREAK"
     gene            complement(8925..10403)
                     /gene="wzxC"
                     /locus_tag="ACHLIF_000551"
     CDS             complement(8925..10403)
                     /gene="wzxC"
                     /locus_tag="ACHLIF_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO9604621.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVACVAVFLLSDAI
                     GDMLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPALHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(10405..11799)
                     /gene="wcaJ"
                     /locus_tag="ACHLIF_000552"
     CDS             complement(10405..11799)
                     /gene="wcaJ"
                     /locus_tag="ACHLIF_000552"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO9604622.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(11935..13305)
                     /gene="cpsG"
                     /locus_tag="ACHLIF_000553"
                     /gene_synonym="manB"
     CDS             complement(11935..13305)
                     /gene="cpsG"
                     /locus_tag="ACHLIF_000553"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO9604623.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(13498..14934)
                     /gene="cpsB"
                     /locus_tag="ACHLIF_000554"
     CDS             complement(13498..14934)
                     /gene="cpsB"
                     /locus_tag="ACHLIF_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO9604624.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPTGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(14937..16160)
                     /gene="wcaI"
                     /locus_tag="ACHLIF_000555"
     CDS             complement(14937..16160)
                     /gene="wcaI"
                     /locus_tag="ACHLIF_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO9604625.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPERIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGELCETFSGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(16157..16636)
                     /gene="gmm"
                     /locus_tag="ACHLIF_000556"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(16157..16636)
                     /gene="gmm"
                     /locus_tag="ACHLIF_000556"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO9604626.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(16639..17604)
                     /gene="fcl"
                     /locus_tag="ACHLIF_000557"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(16639..17604)
                     /gene="fcl"
                     /locus_tag="ACHLIF_000557"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO9604627.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVMRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(17607..18728)
                     /gene="gmd"
                     /locus_tag="ACHLIF_000558"
     CDS             complement(17607..18728)
                     /gene="gmd"
                     /locus_tag="ACHLIF_000558"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO9604628.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(18754..19302)
                     /gene="wcaF"
                     /locus_tag="ACHLIF_000559"
     CDS             complement(18754..19302)
                     /gene="wcaF"
                     /locus_tag="ACHLIF_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO9604629.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(19318..20064)
                     /gene="wcaE"
                     /locus_tag="ACHLIF_000560"
     CDS             complement(19318..20064)
                     /gene="wcaE"
                     /locus_tag="ACHLIF_000560"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO9604630.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(20075..21292)
                     /gene="wcaD"
                     /locus_tag="ACHLIF_000561"
     CDS             complement(20075..21292)
                     /gene="wcaD"
                     /locus_tag="ACHLIF_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO9604631.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(21267..22484)
                     /gene="wcaC"
                     /locus_tag="ACHLIF_000562"
     CDS             complement(21267..22484)
                     /gene="wcaC"
                     /locus_tag="ACHLIF_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO9604632.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVVKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITCTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(22481..22969)
                     /gene="wcaB"
                     /locus_tag="ACHLIF_000563"
     CDS             complement(22481..22969)
                     /gene="wcaB"
                     /locus_tag="ACHLIF_000563"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO9604633.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(22972..23811)
                     /gene="wcaA"
                     /locus_tag="ACHLIF_000564"
     CDS             complement(22972..23811)
                     /gene="wcaA"
                     /locus_tag="ACHLIF_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO9604634.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(23904..26066)
                     /gene="wzc"
                     /locus_tag="ACHLIF_000565"
     CDS             complement(23904..26066)
                     /gene="wzc"
                     /locus_tag="ACHLIF_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO9604635.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(26069..26512)
                     /gene="wzb"
                     /locus_tag="ACHLIF_000566"
     CDS             complement(26069..26512)
                     /gene="wzb"
                     /locus_tag="ACHLIF_000566"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO9604636.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(26518..27657)
                     /gene="wza"
                     /locus_tag="ACHLIF_000567"
     CDS             complement(26518..27657)
                     /gene="wza"
                     /locus_tag="ACHLIF_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO9604637.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            28316..29899
                     /gene="yegH"
                     /locus_tag="ACHLIF_000568"
     CDS             28316..29899
                     /gene="yegH"
                     /locus_tag="ACHLIF_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO9604638.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFCSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHGIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(30173..32026)
                     /gene="asmA"
                     /locus_tag="ACHLIF_000569"
     CDS             complement(30173..32026)
                     /gene="asmA"
                     /locus_tag="ACHLIF_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO9604639.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(32048..32629)
                     /gene="dcd"
                     /locus_tag="ACHLIF_000570"
     CDS             complement(32048..32629)
                     /gene="dcd"
                     /locus_tag="ACHLIF_000570"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO9604640.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(32721..33362)
                     /gene="udk"
                     /locus_tag="ACHLIF_000571"
     CDS             complement(32721..33362)
                     /gene="udk"
                     /locus_tag="ACHLIF_000571"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO9604641.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            33680..36990
                     /gene="dgcE"
                     /locus_tag="ACHLIF_000572"
                     /pseudo
     CDS             33680..36990
                     /gene="dgcE"
                     /locus_tag="ACHLIF_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(37029..37886)
                     /gene="alkA"
                     /locus_tag="ACHLIF_000573"
     CDS             complement(37029..37886)
                     /gene="alkA"
                     /locus_tag="ACHLIF_000573"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO9604642.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            38020..39372
                     /gene="yegD"
                     /locus_tag="ACHLIF_000574"
     CDS             38020..39372
                     /gene="yegD"
                     /locus_tag="ACHLIF_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9604643.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRERLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(39405..41324)
                     /gene="yegI"
                     /locus_tag="ACHLIF_000575"
     CDS             complement(39405..41324)
                     /gene="yegI"
                     /locus_tag="ACHLIF_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO9604644.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQA"
     gene            complement(41321..42082)
                     /gene="pphC"
                     /locus_tag="ACHLIF_000576"
     CDS             complement(41321..42082)
                     /gene="pphC"
                     /locus_tag="ACHLIF_000576"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO9604645.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(42079..42738)
                     /gene="yegL"
                     /locus_tag="ACHLIF_000577"
     CDS             complement(42079..42738)
                     /gene="yegL"
                     /locus_tag="ACHLIF_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO9604646.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(42959..43018)
                     /gene="ibsA"
                     /locus_tag="ACHLIF_000578"
     CDS             complement(42959..43018)
                     /gene="ibsA"
                     /locus_tag="ACHLIF_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO9604647.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(43291..43347)
                     /locus_tag="ACHLIF_000579"
     CDS             complement(43291..43347)
                     /locus_tag="ACHLIF_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9604648.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(43619..43675)
                     /locus_tag="ACHLIF_000580"
     CDS             complement(43619..43675)
                     /locus_tag="ACHLIF_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9604649.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            43954..45201
                     /gene="mdtA"
                     /locus_tag="ACHLIF_000581"
     CDS             43954..45201
                     /gene="mdtA"
                     /locus_tag="ACHLIF_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO9604650.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            45201..48323
                     /gene="mdtB"
                     /locus_tag="ACHLIF_000582"
     CDS             45201..48323
                     /gene="mdtB"
                     /locus_tag="ACHLIF_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO9604651.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVSTTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIASYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            48324..51401
                     /gene="mdtC"
                     /locus_tag="ACHLIF_000583"
     CDS             48324..51401
                     /gene="mdtC"
                     /locus_tag="ACHLIF_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO9604652.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            51402..52817
                     /gene="mdtD"
                     /locus_tag="ACHLIF_000584"
     CDS             51402..52817
                     /gene="mdtD"
                     /locus_tag="ACHLIF_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9604653.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            52814..54217
                     /gene="baeS"
                     /locus_tag="ACHLIF_000585"
     CDS             52814..54217
                     /gene="baeS"
                     /locus_tag="ACHLIF_000585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO9604654.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            54214..54936
                     /gene="baeR"
                     /locus_tag="ACHLIF_000586"
     CDS             54214..54936
                     /gene="baeR"
                     /locus_tag="ACHLIF_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO9604655.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            55127..55459
                     /gene="yegP"
                     /locus_tag="ACHLIF_000587"
     CDS             55127..55459
                     /gene="yegP"
                     /locus_tag="ACHLIF_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO9604656.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            55668..55964
                     /locus_tag="ACHLIF_000588"
     CDS             55668..55964
                     /locus_tag="ACHLIF_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO9604657.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            55966..56262
                     /locus_tag="ACHLIF_000589"
     CDS             55966..56262
                     /locus_tag="ACHLIF_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9604658.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            56365..57726
                     /gene="yegQ"
                     /locus_tag="ACHLIF_000590"
     CDS             56365..57726
                     /gene="yegQ"
                     /locus_tag="ACHLIF_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO9604659.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(58056..58373)
                     /gene="yegR"
                     /locus_tag="ACHLIF_000591"
     CDS             complement(58056..58373)
                     /gene="yegR"
                     /locus_tag="ACHLIF_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO9604660.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            58788..59687
                     /gene="yegS"
                     /locus_tag="ACHLIF_000592"
     CDS             58788..59687
                     /gene="yegS"
                     /locus_tag="ACHLIF_000592"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO9604661.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(59769..60542)
                     /locus_tag="ACHLIF_000593"
     CDS             complement(59769..60542)
                     /locus_tag="ACHLIF_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9604662.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(60648..61688)
                     /gene="gatD"
                     /locus_tag="ACHLIF_000594"
                     /pseudo
     CDS             complement(60648..61688)
                     /gene="gatD"
                     /locus_tag="ACHLIF_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            complement(61736..63091)
                     /locus_tag="ACHLIF_000595"
     CDS             complement(61736..63091)
                     /locus_tag="ACHLIF_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO9604663.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(63095..63379)
                     /gene="gatB"
                     /locus_tag="ACHLIF_000596"
     CDS             complement(63095..63379)
                     /gene="gatB"
                     /locus_tag="ACHLIF_000596"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO9604664.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(63410..63862)
                     /gene="gatA"
                     /locus_tag="ACHLIF_000597"
     CDS             complement(63410..63862)
                     /gene="gatA"
                     /locus_tag="ACHLIF_000597"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO9604665.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(63872..65134)
                     /gene="gatZ"
                     /locus_tag="ACHLIF_000598"
     CDS             complement(63872..65134)
                     /gene="gatZ"
                     /locus_tag="ACHLIF_000598"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO9604666.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(65163..66017)
                     /gene="gatY"
                     /locus_tag="ACHLIF_000599"
     CDS             complement(65163..66017)
                     /gene="gatY"
                     /locus_tag="ACHLIF_000599"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO9604667.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
BASE COUNT        15801 a        17577 c        16512 g        16243 t
ORIGIN      
        1 ccttttttat ctttttctga gattgctatc ggtttatttg gccaatcaat catcaaaaaa
       61 gaatcatccc attttatgca accttctgat tcaggatgat aataatttgt cgtcttataa
      121 acaaactctg ctatctcgct taacaccaaa aaaccatgcg caaacccctc agggatccac
      181 aactgacgct tattctcagc agacaaattc accccaaccc atttcccaaa tgtaggtgac
      241 gatttacgaa tatcaactgc tacatcaaat acttcaccga ccacacaacg aactaattta
      301 ccttgagcat agggttccag ctgatagtgc agtccgcgta acacaccctt agatgattta
      361 gaatgattat cctgaacaaa ttcaaccttc cgccctacag cttcttcaaa aactttctga
      421 ttaaagcttt ccataaagaa accacgctca tcaccaaaaa ctttcggttc aaaaatcagt
      481 acatctggaa tttctgtttt aattacgttc attttattaa taacctttaa tcattttcag
      541 cagatactga ccataagcat ttttcttcaa cggttcagct aatactttca cctgctcagc
      601 atcgataaac cctttacggt aagcaatttc ttccgggcag gaaactttca gcccctgacg
      661 ttcttctatt gttgcgataa aattgcttgc ctcaataaga ctttgatgtg tccccgtgtc
      721 cagccatgca taaccacgtc ccatcatggc aacggataaa cgcccctgtt ccatataaat
      781 acggttaata tcggtaattt ccagttcacc gcgggcagaa ggcttaagat ttttcgccat
      841 ttcgacaacg tagttatcat aaaaataaag cccggttacc gcataattac tttttggttg
      901 tagcggtttt tcttccaggc tgatcgccgt accgttttta tcaaactcaa cgacaccgta
      961 gcgttcagga tcattaacgt gataggcaaa taccgttgca ccactttctt tgttaacagc
     1021 gacatccatt aacttcggca gatcgtgacc gtaaaagata ttatcaccta gaaccaaagc
     1081 acaatcatca ccaccgataa actcttcacc gatgataaat gcctgcgcaa gaccatctgg
     1141 actcggttgc actttgtact gaagatttag cccccactgg ctcccgtcac ccagcagttg
     1201 ttgaaaacga ggagtatcct gtggcgtact aataatcaga atatcgcgaa gacccgctaa
     1261 catcagtgta gacaacgggt aatagatcat cggtttatca taaatcggta acaactgttt
     1321 actgacagcc atagttacag gataaagacg agtacccgaa ccaccagcta aaataatacc
     1381 tttacgcgtt ttcatttcat cattcctttt aattcatctt gctccaccat cacgaacaag
     1441 atgcaaaaac tattaaattg ctgtagtcgt aaataattcg ttgagcattc gtttcacgcc
     1501 aacctgccaa tcaggcaaaa caagcgcaaa tttctgctga aatttttctg tattaaggcg
     1561 agagttatgc ggacgacgag ctggtgtagg ataggctgtt gttggtactg cgttgagctt
     1621 gttgagtgca aggggaatac ctgctttgcg tgcctcttca aaaaccagcg cagcataatc
     1681 gtgccaggtt gtggtaccac tggctaccag atggtacaag cctgcgactt ctggtttatt
     1741 cagtgcgaca cgaatggcat gtgctgtaca atcagccagc agttcagcac ctgttggcgc
     1801 accaaactga tcattaataa ccgctaattc ttcacgctct tttgccagac gtaacatcgt
     1861 tttggcgaag ttatttcctt ttcctgcata gacccagctg gtccggaaaa taagatgctt
     1921 tgcgcaatat tcctgtaacg ctttttctcc ggctaacttg gtttcaccgt aaacatttag
     1981 tggtgcggtt gcatccgtct cccgccatgg catatcgcca tttccaggga agacgtaatc
     2041 agtcgagtaa tgaataagcc aggcgccgac ttcattggct gctttcgcaa tcgcttcgac
     2101 acttgttgcg ttaagtaatt gtgcaaactc cggttctgat tctgctttgt ctactgcggt
     2161 gtgagcggct gcgttgacaa taatatcagg ccgaatgctt cttacggttt cagctacacc
     2221 ttcaggatta ctaaaatcac cgcaataatc agtagagtga acatcaagag caatcaaatt
     2281 acccagaggt gccagagcac gctgtagttc ccaacctacc tgccctgttt tgccgaaaag
     2341 gaggatattc attactgacg gccctcatag ttctgtgcaa tccatgactg ataggcacca
     2401 cttttcacat tttcgaccca ttttgtattg gacaggtacc attccactgt cttccgaatc
     2461 ccgctctcaa acgtttcctg cggtttccag cccaattcgc ggctaatctt ctcagcatca
     2521 atcgcataac ggcgatcgtg tcccggacga tcggcaacat aagtaatttg ctcacgataa
     2581 gattgctctt tcggtacaat ctcatccaac aaatcacaaa tagtcagcac aacatcgatg
     2641 tttttctttt cgttgtgtcc gccaatgtta taagtttcac ccgctttacc ttcggtgaca
     2701 acggtatata acgcacgcgc atgatcttca acatatagcc aatcgcgaat ttgatcccct
     2761 ttgccataaa taggtaatgc cttaccttcc agagcattaa gaattaccag cggaatcaat
     2821 ttttccggaa agtgataagg gccgtagtta ttcgaacagt tagtcacaat ggtcggtaaa
     2881 ccataggtac gtttccacgc gcggactaaa tgatcgctag atgctttgga tgcggaataa
     2941 ggactgcttg gcgcgtaagc tgtcgtctca gtaaataagg gtaattcttc tgtattattt
     3001 acttcgtcag gatgaggcaa atcaccatag acttcgtcag tagaaatatg atgaaaacgg
     3061 aagctatttt tcttgtcgct atcaagagca gaccagtaat tgcgagcggc ttccaaaagg
     3121 acataagtac caacaatatt ggtttcaata aatgccgcag ggccggtaat tgaacggtca
     3181 acatggcttt cagcagccag gtgcatcact gcatccggct gatgctgagc aaaaatccgc
     3241 gccattgcag ctgcatcgca aatatccgca tgttcaaaaa catagcgttc agaatcagaa
     3301 acatcagcaa gtgattccag gtttccggcg tacgttaatt tatcgacatt aacaacacta
     3361 tcctgcgtat tatttataat gtgacgaact acagcagaac caataaatcc tgcgccacca
     3421 gtaacaagta tcttcacttt attattccat attacttcag agcatgctgt gaaataagcg
     3481 gctcttaggt tgattaatag gggtattaat gcacgctacc gcccctggct ttacagctac
     3541 cagtgcactg catgcatgtc tacgatgtga cgagcgttac ccactcgcgc taaacccgaa
     3601 aaattcaaac gctaattgtc ttaccaatcc gctctggaaa caaggaaaat cctggaaaac
     3661 tttgactaaa atcctattgc taactcgttg ttattctgat tgtttatata aaacaacggc
     3721 aggaatattc gcaacaaatt actttcatca cgaatcttca ctgccgttat aattttctta
     3781 tcaaccgtta catccggtca gattttcatt atttgcttaa cagcttctca atgcctttac
     3841 ggaacttcgc cccttctttc aggtttcgta atccatactt cacgaacgcc tgcatatagc
     3901 ccattttttt accgcagtcg taactgtccc cagtcatcag catggcatca acggactgtt
     3961 ttttcgccag ctcggcaata gcatcagtca gttgaatacg tccccatgca ccaggctgag
     4021 tacgttccag ttccggccaa atatcggcag aaagtacata gcgaccaacg gccatgatgt
     4081 ctgagtccag cgtttgtggc tgatccggtt tttcgataaa ttcaacaatg cggctgactt
     4141 taccttcgcg atccagcggt tctttggtct ggatgacaga gtattcagag agctcacccg
     4201 gcatacgttt tgccagcacc tggctacggc ccgtttcatt gaaacgcgca atcatggcag
     4261 caaggttgta gcgcagcggg tcggcactgg cgtcgtcgat cacaacgtcc ggcagtacca
     4321 cgacaaatgg attgtcacca atggcgggtc gtgcacacaa aatggagtgg cctaaaccta
     4381 aaggttcgcc ctgacgcacg ttcataatgg tcacgcctgg cggacagatg gactgtactt
     4441 ccgccagcag ttgacgcttc acgcgcagtt caagaagaga ttctaattca taagaggtgt
     4501 cgaagtggtt ttcgaccgcg ttcttggacg catgagttac caggaggatt tctttgatcc
     4561 ctgcagccac aatctcgtca acaatgtact gaatcattgg cttgtcgacg atcggtagca
     4621 tctctttggg aatcgcctta gtggcaggca acatatgcat cccaagaccc gctacaggaa
     4681 taactgcttt taaattcgtc attatttcat ccacctgtaa aatggttgct gaattatagc
     4741 ttgttcgatt tttttcgcca gcatcaatta ccctgaattg attactgaat tacttgtgat
     4801 gttacgccgc ttcgttgtag attgcagtag cattgttcct aagtatggct ccatttttcc
     4861 aggaatggtc gcaaatctac tccctccgtt ccggcaatct aaagttaatc ttttccacat
     4921 taacaatatg gtgatttatc ctgtcgatat cgacggagct ttgccctctt tcattcaccg
     4981 catgaacatt tgcaagagat agcagtgttt cttttttcgc cataaacacg ccgcgaacgt
     5041 ctttgcgcat gtcgaagttc atgctcaatg cgggtccaac agaggattcc tgcatcactt
     5101 tgatattacg cataaaaagg tgttgcggct tgttgtgtaa ctccagcgac gcacgcttca
     5161 tctcaacgtt agtcagcgcc acaaaggaga cagcattccc ggcggagatt tggatgccgc
     5221 gcaatttgta agcaaggtgg gtattatcca gctgaatatt attcactcgg aaattctgcg
     5281 gtatcgagag atatttgcct ttaattaccc catagccgat taacatcccg gcgctattaa
     5341 tcatttcaat attatcaatc acgaaattgt cacaaccgta aatagcgacc gtggcgttat
     5401 caatacctgc tttcttactg aaatccggcg tgatattgca ggctttgata ttacgaataa
     5461 caaaatgttt accattttca acatgtatca actgccgaca atccgatccc gtgatgttcg
     5521 ccacgacaaa gtttttcact gcctggtctt ccggatagtt gttatcataa gtgcttcccg
     5581 caagacctat gccgatgccc caattgattt tgccgttagt acagttgatg cgctcgatga
     5641 catggtcaga tatcaaaata tcacggtcgt taatcgccac attccattca atggcgtcgc
     5701 cctgtaagtc gctgaactta caattggtga tgttggcacc gataatctgg ttatgaaatc
     5761 cctggcgtaa gatggcgtaa ttagcgtggc taacggtcag gttatcgatg gtcaggttac
     5821 gcatgacccg tttgtttttg ccgccgatat aaatctgcgt taccggacca aagccgctca
     5881 tcgccagccc tttgatggtg cagtcagagc cgcgcacatc cagggtgatg ttatgcatac
     5941 tgccgccctc ctcccctgtc acctggctgc cgtcctgtaa gacaaatcgc cctctgccgt
     6001 tgccgcgcag gcttccaagg atgtgtaacg ttttgccagg agggataaag atgccggtgt
     6061 tgatattgag gcaaaccaat ccggcaggca cgacgaccgt ttgcccttcg ctgaaggctt
     6121 gtttaaatga ggcgatccag tcgcgagggt tgtagtcgtt aatgttaacg ctttgtcggg
     6181 cgggaagcgc gcgagcgaaa ggggtatgga ggaaggcaag caccgagctt gccgtcagga
     6241 aggtgcgtcg ggagagtttt ttaaatggca tgcgttctcc tctataaagc ctgcagcaag
     6301 ctggcgagtt ctcgattgat cacctgctgg ttaaaatcgt gttcgacttt ttcgcgcgca
     6361 cgtttgacga ccggagccag ttcgtcggtg tccagttggc taaacgccgc cagtcgttgc
     6421 gccagtgcgc gagcatcgtt ctcaggcacc agccagccgg atttatcggc ctccaccagt
     6481 tccggtattc cgctatgcag agtagaaacc accggaatgc cgaccgccat cgcttccatt
     6541 agtgccaccg gaataccttc catatcgcca tccgcacctg taaccgatgg caacaggaag
     6601 acatccgcgt cgtcgagcat cgctttcact tcgtggctcg gtttaaagcc cggcatctct
     6661 accacatctt ccagttgata ttgttcgatg agggtgcgca ggcgtcgttc ccacgggcca
     6721 atgccgagga tgcgatagcg aaatgccacg ccctgctctt tcaactgacg gcaggcttca
     6781 atcgccacat gcaggccttt tttctcggtt aagcgtgcga cggaaatgat ttccagcggc
     6841 gttgcgggcg ctttcaccgg acgcgggcta aaacgcgtca tgtccacgcc catgcgcgat
     6901 acggcgattt tttccctcgg gcagcccatt ttttgcaacc ttccggccca cagatcgctt
     6961 attggtagca tcagatcgcc acggcggaac agttgctgat attcgggagt gtaatggttg
     7021 agcacctccc gactggagat atcaatgccg tggaagatag tggcaatttt gccgcgaatg
     7081 acacccagtt cgcggagttt tgctgcggtt acccccgcag ggccaaaatg agcgataaag
     7141 acatcggcat aaaatggtgt tgcgacctgg ccgcaaatgg cagacaaaat caggtttcgc
     7201 gactcggcac catagcgttt gaggttaagt gcctgccagg tatttttacg atgaatgccg
     7261 cgtaagatct ggctggcacg gtggcgcagt ttcgccactt tgcccgtagg ttcgtcctgt
     7321 aaccaacggg ttctggcggc aaggttgtat ttcgtccatg ccgcgtgggt gttctgggtg
     7381 tcgccttttt gcagcgcgac aatctccacc tcaaagccca tatcaataaa cgcggtaatt
     7441 tgattgagga cgaaggtttc cgacgacagc ggaaatttca gtaaaaagaa gccgaccttc
     7501 atttcgcctc cctgatgtgt tcaagcacag atttcaccat ctgcattccc gtctgacgtt
     7561 cgcgactgac ggcttcgtta agtcgcgtgt tcagtgccgg aagctggcct aaggtatccg
     7621 caaccatcac ttgcaggctg ccgtctaata aatgacggat atcaatcgcc atctccggta
     7681 gtcccagctg ctgcataatc ccggcggatt tatgttcata attgatggca atcgccgggg
     7741 tggcaaagtt cattgagata atggcggagt gcaggcgcgt accgatggtg agttcacagg
     7801 cccccagaat tttgcccatt tccagatcgt tgagttcatc catcactacg tggtaacggg
     7861 caggatcgct gatgtgctgg cgcaggttga gcgccaccat gcggtcgtct ttgttatagc
     7921 tgtcgatacc cgtacaggtg gagagcgcaa tcacctgata gccttcgtcg agaatgcgat
     7981 tgaccacccc ggcaaaggct ttttcatatg cttgttgagt ggtgccgaga cgtttgtcga
     8041 acggtgccag ttcgcgcagg gtaattgcca ccgttttctg ttgtgcggca acgtccagcc
     8101 agtgttgaac agcatagctg gcggtgaagt cttctgtgtg gtgatcgacc agccacgcgg
     8161 tatcgacgcc atgttcaact tttgaagtgg tgatattgct gcgtttcatc agttcaaggc
     8221 tgaccgattc gcgcaggatc agcgcgtcgc agtgaccaaa aacgtagttc gccagttggt
     8281 taaattgctc atcctggaac gggccaacac tgtgaccaat cataaacagc gactttttcg
     8341 ccataaacgt gcaaagtgca tgttcaaact gtggcacgcc gtagagatcg acaaaaaacg
     8401 atccgccgac ctgaataatg gcgtcgtaac ctgacagcag gcgcacgaag tcggtgaatc
     8461 cctgggcgat ggcgatatta cgcagcttgc cactgtcggt gacgcgtgag agcaatacct
     8521 gatgctggta gcggcgacgg aggacttttt taacgcgccc gacaacgccc gccgcgctgt
     8581 tgtgttgttt catttgcagg aacagcggat cgcccattac cgggcggttg agtagccagg
     8641 aagaactgac aggatagcgg ctcatcacgt ccacttcggc atgtggattg aggatgttga
     8701 tggcatcaag taagccgcgc aagatggcgc tgtcgccacg attgccgcaa gtgtggttgc
     8761 ccagaataag taatttcatt ttttcctcat aaatttgatg ccaggtgagg ctgtgtttgt
     8821 tgtgttcgtt gccgtttttt gcctgatgcg agcacagaac cgtaggacgg ataaggcgtt
     8881 ttacgctgca cccggcaacc gttgtcggaa ccgaaaacaa caactcaccc cgcccgtaaa
     8941 agcattttca ttttttcgct gcgacaaaac tgacgcttca cttccaccac cagcggatgg
     9001 cgcgacagca caatcatcac cacaaacgcc agcacacccg cggctatttg caccgccagc
     9061 agcatcccca gcgccagttg ccctttcagc acaatgccca gcgcataact gaccaccagc
     9121 gttggcagcg agagataaaa cggcagccac aaactcagga tgtactggcg ataactggaa
     9181 ccaagaaccg gtttaatcat cacgaaatag ctcagaatag tgttgataat ctgcaccagc
     9241 aggaagccaa gcgtgacgcc gattgcgccc gccatctgcc caccaataac aatcgccgga
     9301 ataaacagaa atgttttaaa tacgttgaat ttaaagctga tatcgacccg cgctttcgcc
     9361 atcagcagcg aaccaatcgg gttacccacg gagcgcagca gacccaccac acacagcagc
     9421 tgtagcaccg gaataatgct gctccacttt tcgccaaata ccagcggtac aaagttattc
     9481 gacaccacca tcagccccag cagcgccgga aagttgataa tccccactac cgacagcagc
     9541 ttgtagaagt tgacgcgcag cttttcggta tcgtcctgaa ttttggcaaa cgccggaaac
     9601 aacacgcggg tgatgatcgg gttcagtttc atcggcggca caacggccac gttgtacgcc
     9661 aggttgtatc ccccagccac gcccgcgccg agaatacgcg ccagcacgag tgttgaaagg
     9721 ttggtgttga gatagttgat gatgctgtcc gccgtcagcc aggcaccaaa gcgtaagttc
     9781 ggtgccaccg acgccagcga gaaatgcaga gcggggcgat agattttgcg gccaaagtag
     9841 ccaaacagca gcgttctcac cgcactattg accagataac cgaggatcgc ggtcatcgcc
     9901 agcggccaga aatgggcgct aaccaccgta aaagtaaagc ccgccagcac cgcgctggtt
     9961 tcgatcatgc cgattttgtt gaactccagc tctttttgca tcaacgcgcg gaactgttgc
    10021 ccgtggggga tcaccacaaa cgctagtgac aatgttttaa tcaacggtgc caggtccggg
    10081 ttattcagca tgtcgccaat ggcatcactc aacaaaaata ccgccacaca cgccacgatc
    10141 cccagcccga cgttcagcca gtacaacgtg gtgagttcaa ggtggctgat ttcttttcgc
    10201 tgaataatgg agttagcgat accgaagtca gaaagcgtat ctgccagcgc gataatcacc
    10261 agcgacacag tcagcaaacc gaactggtgg ttgtcgataa tccgcgccag cacagtcatt
    10321 tgcaccagcc cgaggccgat gatgatcacc gtggcaatcg ccgaccactt cgcgccgctg
    10381 atggtttttt cacgtaagct catatcaata tgccgctttg ttaacgaagc ctttgaatac
    10441 cgtcaggaaa acgattttga tatcgaacca gacgctccat tcgcggatgt actcaaggtc
    10501 gaactcgacg cgtttttcca ttttctccag cgtgtcggtt tcgccgcgcc agccattaat
    10561 ctgcgcccag ccggtaatgc ccggtttcac tttgtggcgc agcatgtagc cttcaatgag
    10621 ctggcgatac tgttcgttat gcgctactgc gtgcggacgt ggaccgacaa tcgacatccc
    10681 cccggtcagc acattgataa actgcggcaa ttcgtccagc gaggtgcggc gcagaaagtt
    10741 ccccactttg gtgacgcgcg gatcgttctg cgtcgcctga gtcaccactt tgtcgttctc
    10801 catcactttc atcgaacgga acttccacac tttgatcggc ttgccgtcca tgccgtagcg
    10861 agtctggcgg aaaataaccg gccccggtga gctcagtttc accgccaacg caatgcagca
    10921 cagcaccggg gagatcagca gcagaatcag cgttgccagc acaatgtctt ctgcacgttt
    10981 gagcaggcgg ttaaccccgg aaagcggcgt gtcatacagc ggcaccactg gtacgccgtt
    11041 catctcttca atacgggaat ggagaatgtt gaaggtaaag acatcgggga tcagcagcac
    11101 cgaacaggta gtgtccgcca gttgatggac cagttttttc actcgcgcgc cgtcgcacat
    11161 ttgcatcgcg atatagacgt tatgaatctt gcccgctttc gcatcctcga ccagctgttg
    11221 cagattgccc gcccagtcgt tagaaacgcc acccggtttc gggtcgtggt aaacgcccac
    11281 cacttcaaac cctaaccacg gctgattacg gaagctctcc atcagcattt gtccggcggc
    11341 taaatccccc gctacggcga ccatacgctt gttatagcca tgattacgca gccagcccgc
    11401 gccaatgcga atacatgaac ggcaaaccac cagtccgatg ctggtcagcc cataccacgc
    11461 cagccagatt ttcagttgcg tgtcgaaatc attattgaac gccaccagtc cggcgctgaa
    11521 aatcacgctt aaggtccagt tttgtagcaa cagggcaaat tctgtcgctg cccgaacacc
    11581 gcgccatgag cgataaaaat cggtgatgcc gcccagcatc tggaacacca ccagcgtaat
    11641 caacgccacc aacaggtgca tgtagaggaa tgacagtccg ctcacttcgc aaaccagcca
    11701 tagtccggca aacatgatgg tgatatctga aaagcgttgc accatagaga ttaacgatgc
    11761 attggttttc gctcgctcgc gcttttttag atttgtcatc gttgttcctg ttctattgtc
    11821 gcctcgcccc tcaccccggc cctctcccca aaggggcgag ggggagaaga aagttccgtg
    11881 ctcacttctc tccgcaggca gtagagcgtc agggtgaggg ggaaagtaat gacattactc
    11941 gttcagcaac gtcagcagag ttcgcgttcg cgcttccatc agcggtacat caccgcgcga
    12001 ttccacattc aaccgcacca ccggttcggt gttggaggag cgcagattaa agcgccagtc
    12061 ggcaaaggtc atgctgatgc catcggtgcg atccaccgcc agcgcctcac ggctaaagtg
    12121 ctgttccacg cggttaatcg cctcaacggg ttgcgccagt ttgctgttga tctcaccgct
    12181 tgccggaaac gccgccatcc ggtcgcgcac cagttcgccc agcgtttttc ctttcaggca
    12241 caccagttcg gcgaccagca gccatgggat catgccgctg tcgcagtaag cgaaatcacg
    12301 gaagtaatgg tgggcgctca tttcgccacc gtagatggcg tcttccttgc gcatacgttc
    12361 tttaataaag gcgtgtccgg ttttcgacat taccggggtg ccgcctgcgg cagtcaccac
    12421 atcaacggta ttccaggaga gacgaggatc gtggatgatc ttcgcgccgg gatttttttc
    12481 gaggaacgct tctgccagca ggccgacaat gtagtaaccc tcaataaact gccctttttc
    12541 gtcaaacagg aagcagcggt caaaatcgcc atcaaaggca atgcccatat ccgcgccgtg
    12601 tttgatgacc gcattgcggg tgtcgtcgcg gcattccggc agtagtgggt taggaatacc
    12661 gttggggaaa ttgccgtccg gcgtgttgtg cactttgatt aattccacgg gtgcgccgag
    12721 ggctttaaag cgggcttcaa tggcgtccac caccggaccc gctgcgccgt tcccggagtt
    12781 gatcaccagc ttgagcggcg tgaggttttt gacgttgata taaccgaaca ggtgatcaac
    12841 gtaagcgtca cgcaggttga tttgctgata gcgaccgcgt ttggtttcat cgacgggagg
    12901 gaagtcgttg gcttcagcca gacgctggac gtcgcgcagt ccggtatctc cgctgatcgg
    12961 gcgagccccc tcgcgaacca gcttcatgcc gttataatcc atcggattat ggctggcggt
    13021 aacttcaatg ccgccatcca cgccgagatg gaacgtggcg aaatagatct cttcggtgcc
    13081 ggacatgcca atatccagca catcgacgcc cgcatcctgt aaacctttcg ccagcgccag
    13141 ttttaaggtt tcgctggtga ggcggacatc accgcctaac acaatggttt tcggtttgag
    13201 aaattcgcca taggcgcgac caatgcgcca ggcaatatct tcattcagtt cttcgcctaa
    13261 tttcccgcga atatcatagg ctttaaagca ggttaatttt ttcatatcgt tacccttttt
    13321 caggcaatag ttggccctga ccgaaacggc caattttgtt tttgtcgtta tttacatcgc
    13381 ggtttcgcat tcattgcctg atgcgacgct gccgcgtctt atcaggccta caaatccgaa
    13441 ccgtaggtcg gataaggcgt tcacgccgca tccgacaaac agcgcctgat gcgacgttta
    13501 cacccgtccg tagcgatccg cgaagcgcac cacatcatcc tcttcgagat aagagccgga
    13561 gcgcacttca attaaatcga gcggaatttt ccccgggttt tccaggcagt gcgtcgcccc
    13621 cagcggaata taaatggact cgttttcacc aagcagtttg atatcaccgt caatagtgac
    13681 ttttgccgtt cccgcgacca ccacccagtg ttccgcgcgg tgatggtgca tctgtaccga
    13741 caagccttcg cccggtttca cggtgatgcg tttcacctgg tagcggtcgc ccgcgtcgat
    13801 agagtcatat ttgccccacg gacgatacac ttcgcgatgt acccgatgct catggcgacc
    13861 atcggctttg atctgctcga ccactttttt cacatcctgc accgcgttac ggtcggcaat
    13921 cagcaccgca tctttggtct gcactaccac cagatctttc accccgacgg tggttacgag
    13981 gccagattcg gcatacacat agctgttttc tgttttgtga ttaatcacat cgccgtggca
    14041 aacgttgccc tcggcggtgt gggcgctgat ctcccataat gaagaccagg aaccgacatc
    14101 gctccagccc gcatccatcg gcaccacaac ggcatctgcc gtacgttcca tgaccgcgta
    14161 atccaccgac tcttccgggc aggcgagaaa cgcttcttca tccacgcgaa taaaatcgag
    14221 atccggatcg acggcgctca tcgctttttc gcaggcatcg aggatatccg ggcgatattt
    14281 tttcagttct tcgagatagc gtccggcgcg gaacaggaac atgccgctgt tccagtaata
    14341 ttcgccgctt gccacatagg cctgggcggt ttccagattc ggtttttcga caaactgcgc
    14401 cacttcaaag gccaccgtat cctgctcacc cgcagacact tcaccgcgac gaatatagcc
    14461 ataaccggtt tcaggtagat ccggcacaat gccgaaggtc accagcttgc ccgcttcggc
    14521 atatggcatg gcattacgca cggcggcacg gaacgcgtct tcatcggcaa tcacatgatc
    14581 cgccgccaat accagcatta acgggtcgct ctccgggcta tgacgttttg ccgccagcgc
    14641 cgccagtgca atggcaggtg cagtgttacg ccctgtcggt tcgagaataa tgttctcggt
    14701 gagtttgttc agttgacgca gctgttccgc gacaataaag cggtgctgct cattgcaaat
    14761 caccaccggg ctttcgcact ccacgccgtt caggcggcag atggtggttt gcagcatggt
    14821 gagatcgcct ttcaggcata aaaactgctt ggggtaaagt acgcgggaaa gcggccataa
    14881 gcggctaccg gagccacctg ccatcacaac tggatagagt ttcgactgcg ccataattat
    14941 ccccgaatat catttataaa ttgacgtaac acgttctctt tatcgagcgt gcgttcggca
    15001 tattcacgtg ccaccgtgtt gtgtttgggc agcaggagcg cctgacggat ccccgccacc
    15061 agcgcttcga ccgattccgg ttctacgcaa accgcaatgc ccgaaaaggt ttcgcaaagc
    15121 tcccccagtt ctgtgtgggc ctctgcggta atcaccgcgt taccgcctac cgccagaata
    15181 ttggtcagtt tcgacggcaa tacggcatct gccgcgccgc gtttttgcac caccagatgg
    15241 caatcgccca tcttcagcag tgcgggtaaa gcgtcatacg attgcagcgg gaaaaattgc
    15301 atgttgtgca gtccacgctg ctgcgccatt ttttccagtc gcgctttgcc gccaccctgc
    15361 ccgacaatgg caaaaatcag tggttcatcg cgcaggcgat cggcagcttc aataacgttt
    15421 tccagcccct gcttttcacc aatattgccg gagtaaagaa tgattttttt gttatccggc
    15481 aggccaagct ggttacgaag ggcatcaaca tcggcatctg caacatgctg aaaacgggcg
    15541 atttccgacc agttggggaa gaagatgacg ttttccgccg ccacgccttt ttcgatggct
    15601 ttattcatca tcgaacgcga aatcgtggag acgttatcga cgttatgtag tccgctacgt
    15661 tcgaacgccg ttgccagctg tgccactttg ccgcctttgc cttttccggc aaggcccagc
    15721 cccagcatgg catctacttc gtaatcctga atatgcagca cggtacgcgc gccagagagt
    15781 ttcgccagca gacgcattcc cggcgtgcaa aacagcgttg gcactacgcc gataatgcgc
    15841 tccggcttcc agcgacgttg cgccatcagc gggaaaaaac tgctgacggc aaaactgccg
    15901 agatgcaaca ggcgtttcag ggtgctcggc tgttttggca catacagcgg gcagcgccac
    15961 accgtggcgg ccccctcttc tcgtttgtag cgccaggcgg aatagttctc gcccacctgc
    16021 cactgcgggt agtaaggcgg tgcggtaatg acccgcacct catgaccttg tgccgccagc
    16081 cattccacca tctcgccggt gtatttgccg atgccggtta actccggcga gtagttaatg
    16141 ccgtagacca gtattttcat aatccgggta ctccggcacg cttctcagcg agaaaatagg
    16201 cgcggctgtt agcgtgaaca ttgtcactgg cgagcagcgc gtccggcgtc agccagcggt
    16261 aatcgtcatg ctgctcatcc ggcagtaaca gatcttcttc agcgactctg aagcgaaaac
    16321 cgagcaccac atagtgagtg gtgaaatccg tgccggagaa gttatcgtca taaaagtgct
    16381 gccagacacc gtaaaactgg cctgctgtta tcggcagacg cagccccagt tccgccatcg
    16441 tcagccgctc aaatgcggct tccagcgttt cgtctttctg cacgcgccct cccggcacaa
    16501 accagtaacc ctgcgccggg cggttggttc ttttgccaag cagaaactcg ccgcgactgt
    16561 tctcgacaat aaagtcgaga gagacaagcg gtgtggagcg cactaccgtg gcaaagtctt
    16621 cctgacgtaa aaacatcatt acccccgaaa gcggtcttga ttctcaagga accactggta
    16681 agtgctggca agccccgctt ccagtgatat ttcgtgatac cagccaagct gatgcaggcg
    16741 catcacatcc agcagtttgc gcggtgtgcc atccggtttg ctggcatcaa aaaccacccg
    16801 acctttgtaa cccaccactt tggcgatggt ttgcgccagc tcgcggatag tgcagtcaac
    16861 gcccgtgccg acgttaatgt gcgacagcat cggctgggtg ttctccagcc agacttcgtg
    16921 cgccagctcc atcacatgaa tgcttgccgc cgccatatca tcgacgtgca ggaattcacg
    16981 catcggtgta ccgctgcccc acaccaccac gtccggcgca ttctgtgccg tcgcctcgtg
    17041 gaagcgacgc agcaatgcag ggatcacatg cgaattactc gggtggaagt tgtcgtgtgg
    17101 cccgtacagg ttggtcggca tgactgaacg gtaatctcgt ccgtactggc gattgtaaga
    17161 ttcgcacaat ttgatcccgg cgattttggc aatagcataa ggctcgttag tcggctccag
    17221 cgtgccctgc aacaactcgc tttctgccat cggctgtttt gccagtttcg ggtagatgca
    17281 ggacgatccg agaaacagca gtttgttcac gtcgttctga tgcgcggcgt gaatgatgtt
    17341 gctctcaatc atcatgttct ggtagatgaa atccgccgga taggtgttgt tggcaacaat
    17401 gccgcccact ttcgccgccg ccagatagac ctggtcaata cgttcgctgg caaagaaatc
    17461 atgcacggcg cggctgtcca gcaggttcag ctcgtcgcgg gtgcgtaata ccagttccac
    17521 atcaccgcgc tgttcgagct gtcgcttgat ggcggaaccg accatcccgc gatggccagc
    17581 gataaaaatt cgttgtttac tcatgcttat gactccagcg cgatcgccac gtcgtagccg
    17641 tgagatttca gcagagagtg ttttttcgcc gcttcgaggt cattagccac catttcagac
    17701 accatctctc tgagggtgat ttccggtttc cagcccagtt tttcgtgcgc tttggtcggg
    17761 tcgccgagca gcgtttcaac ttccgccgga cggaagtaac gcgggtcaac ggcgataatc
    17821 acatcgcccg gtttaacgcc cggcgcgtca tgcccggtga cggaaaccac aatgcccttc
    17881 tcttcaacgc ccgtgccttc aaagcgcagt ttgatgccca gctgtgctgc cgccatttcc
    17941 acgaactgac gtacggagta ctgaacgccg gtcgcgataa cgaaatcttc tggctgttcc
    18001 tgttgcagca tcatccactg catttttacg tagtctttgg cgtggcccca gtcacgcagg
    18061 gaatccatat tgccgaggta caggcacgac tccagcccct gggcgatgtt ggcgattgcg
    18121 cgggtgattt tgcgggtaac gaaggtttcg ccgcggcgcg gggattcatg gttgaagaga
    18181 ataccgttac aggcgtacat gccgtaggat tcgcggtagt taacggtgat ccagtaggcg
    18241 tacagtttgg cgaccgcata cggagatcgc gggtagaacg gcgtggtttc tttctgcgga
    18301 atttcctgca ccagaccgta cagttcagag gtggaagcct gatagaaacg agttttcttt
    18361 tccagaccga ggaagcggat cgcctccagc aggcgcagcg tacccatcgc gtcgacgtca
    18421 gcggtatatt ctggtgactc aaaagagacc gcaacgtggc tcattgcgcc caggttgtac
    18481 acttcatccg gctgtacttc acgcaaaatg cgcgtcaggt tagaggtatc actcaggtcg
    18541 ccataatgca gatggaattt cgggttgcag gtgtgcggat cctgataaat gtgatccacg
    18601 cgctcggtgt tgaatgacga tgcgcgacgc ttaataccat gcacctcgta acctttttcc
    18661 agcagaaact ctgccaggta agaaccgtct tgtccggtta caccggtgat gagagcgact
    18721 tttgacatgt cttattcctc tgtatttttg aatttattca gtttcaacgc gttcgcgtat
    18781 caccactgcg ggattccccc ggcaaaccac atttgccgga agcgatttaa aaacactgct
    18841 tcgtgcaccc acgacggtgc cgtcgccgat tgtgacgcct ggggcaacaa agacatcggt
    18901 tgccagccag catttctcgc caatcacaat aggcgtggcg ttaatggtga aatgttgact
    18961 tgcatggtcg tggctaccgg tgcataaata acttttttgc gatatcaccg aatgtgcgcc
    19021 aatggttatt tcaccgaggg tatataaatt gacgtcatcg ccgacccacg cgtaatcacc
    19081 taaggttaat ttccacggat aggtaatttt tactgacgga cgaataacta cgttttttcc
    19141 tatttttgct ccgaataaac gtaataaaaa agcccgccag cgatacaata cttgtggcga
    19201 ccaggcaaat attgttgcct gtactgccca ccataattgc actttaatag cgttgccgcc
    19261 ccggaaccct ttcggcaccg agaaaccgct taaatcttgc attggttctc cttatattca
    19321 gactttgtta tataaggctt tcgtctttga ggtagtacgt tggcgtaaat gccaggataa
    19381 ttcagcccaa aagccaggta catgtaatat ttgtcgttgg acttttttcg cgtcagcaca
    19441 caattccata ttattggtgg tagatacccc acccatggaa aattcagaca ccaggccatt
    19501 gagtttttta aatgcataac cggctttata cattttggct gccagcgcgt agtcggaaga
    19561 aactttatat tccaggtcat aacgccattt tttcaagccg gatacaggga aaaatatcgc
    19621 ctgatgactg gcgggcaggc tgtgataaat ataccagccc ggtttggcgc tacgtttaat
    19681 tttatgcccg tcgccaaaat ccagcagcgc atcgccggtg atcatcatgt tatctttttg
    19741 catttttaac ttacggacaa aatttgcggc atcctgatga aaaatatcgc ccgaattgag
    19801 aaacaacgcg aacttgcctt gtgccatcgt aatgcctttg ttcatggcgt cgtagatacc
    19861 gttatctggc tcgctgacaa agcgtaggtt atagatacca ttgagatttt ccagatactc
    19921 acgggtgccg tcattggaac cgccatcgac aacaatccat tcgaagctga tatcttccgc
    19981 ctgcgccaga tgcgccagcg aggcatgtgt tttgactatc ccttcgaggt tacgaaacgc
    20041 gacagtgatt atgctaagca acatgttctt attcttacct cgtaatattt aacgcttttc
    20101 gcaaaataaa cggacagacg attaaaaatg catattccgg gctaaatatc gaaccggtaa
    20161 aaaacagcga caccggagta aaaagatata attgcacgcg aaaattgcgg ttatcaccaa
    20221 aagcgttgat catcattttt atcactttcc ccatgtacca cagcgataaa aacactgcga
    20281 accaggaaaa ataaataatc agcagataca aaccattgtc tatggttttt ccgacatccg
    20341 caccgttaaa tattccgaat gatgcgacat attcataaag tgagccaaat ctgactacac
    20401 cgtcaatatg ggtcaaagaa taaccgacca tcaccagcgg accgacaata cgataatacg
    20461 atgacgatcc ttccgtacct aaatcgccca gacgggtgga aatataagga aacgcgatta
    20521 ccacaccaac caggaataca gccagagaaa tcaatgctaa cggtaacttt ttcttaatcg
    20581 cctctttatt cagatattga aatgcccact ccagcaaata aaacaggata aaggtcataa
    20641 cccctgaaaa cgatccggat aatattatcc ctgcgagaat catagcatcg gttttaggcg
    20701 ttttgatacc aaactgtttg atgctgagcc aaattgagat taatgccaga gcgaaaaatg
    20761 ccggttcgaa ataaagtgct gtcgtgcgct tgccgccgaa tttaatgaaa ttcagtacat
    20821 agctgttgct gtaaatcaga tatttcgaaa tcgactccat aatactgctg ccgccggtga
    20881 ggataatttg cgccatctcc accgccgcca gcgccaccac caacccgacc accagataaa
    20941 agaaacgtaa tatcttgcga tggttgtgtg gcgaaattgt tttaaaacga atactccaca
    21001 ccatgccaat aatgatcaca atatagacaa acagcatagt tgaagtgacg tatttactgg
    21061 catccagcga ctgaccaaac agatagttaa acgccgtgag tcccgcacca atccctaagg
    21121 caatcatcaa ttttttaacg ctgatgcgct ctaaaaacag cagtaacaag acaggtaaaa
    21181 aagtgacgat ggtgatgggg aaactttcgc caagctgggc gattttgacg ttaaccagca
    21241 gataaatcag cggcaacaac aggtagctac agattctgat agaagttgac atactcctcc
    21301 agcatctgtt gtccactgta ggccgcgcgg ctgcgttggc tgaactcagc cagcgtggta
    21361 ccaaatatcg cctgcgcgat ttccggtttg cttaactgca ccagttgcag cacgtcttct
    21421 tcgctgacgg ttttaccgcc ggatttttgc agcacttccc gcgccgcgtc gctatgggtg
    21481 gcaatcaccg gcacgccaat cgatagcgcc tcacacaaaa tcagcgggta gttatcgacg
    21541 cgagaactga acaccagcgc atccatctga ttgagcgcgc tcatcaactt gcgcttgtca
    21601 gtttcaaagc cgtgattaac cacgttgcca gcggtgaacg gcgagaactt accaaaggta
    21661 tgcagttcga ttttgtcgcc cagcgccatc atctcacgca ccagttgctg gttagttttg
    21721 ccgtcgtaac gcaggtcatg cgccaccacc gcgattttcg gcttaccctg ggtttcgcgc
    21781 accggaggca gatccgccag aatcgcttcg gttgccatat caataccgtt attgataatc
    21841 cggcaacgcc ctggaccgta caggctattg aaagcgtcag ccacatgctg gctgggggaa
    21901 ataaactgac agcccagcgc cagcatctca cggaataact ggcgtttgcc cgccaccagc
    21961 tggtgcgccc gatcaatctt caccggcgga taattattta aggtcgggca tttctggcag
    22021 cccgttttcc agccttcaca accgtcagta aaggcgcagc gcccggtaac gctccagtgg
    22081 tcgtgcagcg tccagaccag agttacatcc ggtttgtggt ttttcacctt ttcgcaaaag
    22141 cgcaccacgc tcttaagatt tagccagtag ctgtgcagca catgaaaatg caggaccacc
    22201 ggacccggtg tgcaagtaat ggtgcgatat aactcattga aattgccaaa cagatcgcga
    22261 ttaaacagac gaaacaacgc aatgttcgcc atcgcggtca tccgcggcgt atgtttgacg
    22321 acctgcggat agttttgatg gctgacgctc tctttgccgc ctttgccgta gccgtagaca
    22381 aaatgtgacg ccagcccctg ttgcagcgca cgctgatgga gatctaacgc cacacctgcc
    22441 gccccgcctt ccgccagtcg cacattaaat tgcagaatat tcatttaatt accttcactc
    22501 gcgctttttc tcccaccacc agcgcgttgt ccgggacaga gtcgagcacc acgctgcccg
    22561 cgcccacggt gacgttgtta ccaagcgtga tatcaccaag aataatgacg ttggcaccga
    22621 gttcgacgcc gttgccaatg tgtggacatg ccatgttatc ggcaccacga ttgccgatag
    22681 tgacgccgtg gcgaatggtg aaatcatccc ccgctaccac gtttttattg atcactacgg
    22741 cgtaaccgtg atggatagta aagcggcggc caatggtcgc ggcggcctgg atttcataac
    22801 cgaaaaagca ttcggtgata atgcgataca gcaccagcag cggggccgcc cacagattgt
    22861 tgaggacgtt ctttttgcgc cacaccgaac aaaaatgagc aacacgatag gcaagaacca
    22921 tgcagcacgg gcgtaaactc cagctgttgg cacgcagatc ttccagcatc tttagcgccc
    22981 ccgaatacca tcagccagac gtttgccgtt acgcaccgac agcagcgtca gcaaagtgcg
    23041 ccaggtcata cgtttattgc ggatctggta gagggtaaac agctgatatt ttttgcttgc
    23101 acggtcaaat ttatctttgt gcttgcggta aaagtggaag tacccggaga attttttcgg
    23161 tgacgaggtg atctgcattt caccgtgatt gatatgcagg atctgcgtcg cctcttccac
    23221 tttccacggt tcgccgtact ccaccaccat ccgcaggaag atgtcgtaat cctgtgctgc
    23281 tttcagttcg gtatcgaaca aacactcttt gaaacgccat gcccaggtaa agacctgatt
    23341 gccaatgata ttgcgtttat agaacaggcg gcgtgagtac ggcgatttgg gatacagtgg
    23401 taagcttgcc ggttgggaat aaacttctcc ctggcatacg tagtcgttag cgtacaaaaa
    23461 ggcgtgtgtg accagttgct gtttatgggc gaggaagacg ctcagacggt tgggtgtcca
    23521 ttcgtcatcg tcatcaatcc cggtgatata ttccccttgc gccagcataa tcgcctggtt
    23581 acgtaccgcg cacgccccgc tgttaatatc gttgtgaatg taagtgatac gcggatcgtt
    23641 gagggcggtg acgtactgtt gcagttgttc ccaggaggtg gagcaatcat ccacgatgat
    23701 catctcccag ttgctgtagt cctggcgcaa aaccgatttt attgcccgaa tcgccagttg
    23761 ttggcggttc caggttggca tatagattga gatcagcgga ttgtctttca taggttgttc
    23821 tcccggctta tgagctggtt tgcttgccgg atgcggcgtg agcgccttat ccggcctacg
    23881 gggcggtgcg aatgcaggcg tcgttatttc gcatccgact tatattcgta ttcgtaatag
    23941 ccataatcct gatacgcgct ggcgcggcgg aagatggagt tcagaatcac ccctttcacc
    24001 ggaataccat tttgctcaaa gcggctcaga ctggtttcca cttctttcaa tgtgttgacc
    24061 gcataacgcg ccaccattaa cgtggttcca acatgacgac caacaattgc cgcatcggtc
    24121 actgccagaa tcggcggcgt atcaatcaac accaggtcat agtttttact cgcccagttc
    24181 accagttcgg caaagcgttc gctcatcaac agttcagaag gatttggcgg cacctgaccg
    24241 cgcgggatca ggtcaaattt ggcaatagag gtcggtttag cagcggtagt aatatcgccc
    24301 tgaccaatca gaatttccga caggccatta acgttattag tgcccaacag ctcgtgggtg
    24361 tagcctttgc gcatatcgca gtcgatcaac aacacgcgtt tattggtctg gctgatcacc
    24421 gccgccaggt tggcgcagac aaaggtttta ccgattgacg ggctaacccc ggtcatcatc
    24481 aacacattgt tctgcgcctg catcatcgcg aagtgcaaac tggtacgaag actgcggatg
    24541 gcttcaatcg ccagatcggt tggattcccc accgccagta gctggctctg tttatagcgt
    24601 ttaatccctt tgatggtttt gacgctatcg cgcgctttct gccattccga caacgggatg
    24661 ctggcataga cgctgatacc gtgttcttcc agcacctgcg ggctttcgat gccgcgatta
    24721 aacaacgagc gcagcagcac acccacgata gagagcatca ggccaaggat aatcgcgccg
    24781 aggataatca gccctttctt cggttttagc acaccaggct gagtgattgc cgggtcaaca
    24841 atgcgcacat cgccgacggt gctggcttcg gtgattttca gctcctgctc tttattcagc
    24901 agttgcatat agacctgctg accagattcg acatcgcggg tcagacggac aatttcctgc
    24961 tgggttttcg gcatcgccgt tacgcgacca ttaagtttgg ctttttcgtc ttccagcgcc
    25021 tgacgtttct ccagcagcgt gcggtacgcg gggtgaactt tggtgtacag cttggagatt
    25081 tccgcctctt taaaggtcag ttcgttcaac tgcgcgtcga tgttcaccat cgaatcgagc
    25141 accgctttcg cttccagcgg cagatcaaca gaatctttat cctgacggaa ggcattcagt
    25201 ttgttttcgg caacatcgag gcggctacgt acttccggta actgttgcgc gaggaaagcg
    25261 aggcttttcg acgcttccgc cgatttgcgc tcaatatttt gttcctgata gttacgggcg
    25321 atgctgttaa gaatgtcgcg gatctgttca cgatcttcac cggtataagt caggctcagt
    25381 acgcctgcgt ctttgccgtt ctccgttacc gtcaggctgt tttgcagttg attgatcatc
    25441 cccagcgtgg agtatttggt gacggtaaac tcactgcccg ggcgggcgtg aatggcttca
    25501 accatcagcg tgacgccttc ttttttcagc atctggcccg cttgcccacg ggcgctaaag
    25561 ccgccatcac tgctcagggt gtagttttta ttgtccagca cattaagcgt aaacacctgg
    25621 tccgccatct ctttcgggcg gttaaaggtg gtcactttca ccgtctcgtt ctgacgtccc
    25681 atcaaacgat cccagcccgc accgaaaatc gggaacgtgt ttttgctcac cgcaatatcg
    25741 aggtctagat cgtccaccgt tttaccaagc accagacgcg aacgaatcaa ctggatctcg
    25801 gcgtccgatg caggcggttt gttggctaat gccgagccaa tgtcctgcac taacgaattg
    25861 ccgctgtttt gttcgatttg taccagtgcg tcggcactat aaatcggcgt ggcgaagaaa
    25921 gtgtaaacca cagcacagag ggcaaataca gcggtgatgc caatcaccca ccagcgcgct
    25981 tcaatgacgg tgccgaccag gcgaccaata tcgatttcat cactgcccgt taccggagcg
    26041 gcatgttgtt ttactttttc tgtcattctt atacctgctc tgcgttcaat gcctgcgccc
    26101 actggcgggc agaccgttca agtaatgtgt acaccgctgc aaacgtttcc cggcttttgc
    26161 gatacggatc ggggatttca cattcgttat cccagtgacc aaacagcatc actttgccgc
    26221 gcatctcagg tgccatctcg cataagcgtt cgatatggcg cttttccatg gtcaaaatca
    26281 ggtcatagtt gcgacacagg cggcggctga tttgccgtgc acagtgacct tccagagaca
    26341 gttgatgttc tgcggcgacg ctgatagcgg taggatcagc gcccttaccg accagcgcgc
    26401 cgagtccagc ggactccact ttcagctccg ggtgataacg ttgcagtaag cgttccgccg
    26461 tcggggaacg gcaaatattg ccgacacaga caactaagat gttgttaaac atgacgatta
    26521 ccagttatga atgtcgctgg ctgtatccgt catgtaacgg acaccgctaa tagttggcag
    26581 caactgattg atcagacggt tccagcggga aaccggggcg gtggtgacat acaccacgtc
    26641 ataaggttgc aggcggaatt ctgtcgccat caccagcgac gtcgcatcgg acatatccag
    26701 ctggtagata ttggcaatct tgccgttacg cccgccctcg cctttcaacg gacgaatgac
    26761 aaagatgccg ctggcgttgg aggtggtcat gtcgatgcct tcggcattgc ccagagcttc
    26821 agtcagggtc atgccgctaa agtccatttt cagggtgctc tgtttcttca cttcacccat
    26881 cacaaatact ttcagatcat cattacgcgg cacgtagaga atatcgccgg gataaagcag
    26941 tcggttctga ttgaggtcgc cgttttgcat cagcgcctgc aaagaaatgc gttcttcacg
    27001 accattgtgt gttagcacca cgttgcgcca gtcagcggtg tcggtcaggc cacctgcggc
    27061 gttgatggcg tcgagaatgg tcagtggtac gttggtgatc gcctgttgac cggatttatt
    27121 cacctgacct gagatatagg ccttttgcga gcggaaggcg gcgatattaa cgtccacctg
    27181 cgggtcagcg atgtacgtcg ctaagcgccc ggtaatatca ctgcggattt cagcgagcgt
    27241 tttcccgact acgtgaacct tgccgatata cgggtaaaac atagtgccgt caggctgtac
    27301 ccagttgccg gtgtcgctgg agctgcggta ctgaccggct ggcgtggtga gttccgggtg
    27361 atcccagacg gtgacattaa gaacgtcccc cggcccgacg cgatactgat aattcgcgat
    27421 ctcgctttcc agcgtcatat tggggcgcgc tacattcggg cgtgggcgta attggtcaat
    27481 caggcgcggg gtcagcggat aaacattcac cattttgtcg agatcgaaat cagcgtcctg
    27541 ctgtttgatg acgtctttgc ccatcgtcga catattgctg cccggaagta ctgtgcaacc
    27601 gcttatcaag gttactgaca ccaataatgg catcaatttc attttggatt tcatcattgt
    27661 ttatttatca ctttggcaga gtaattatcc tgtgcactat taatagcaat gtcgccatgc
    27721 acatttacct tgcagttaat tgaataaaaa tttaactggc atcagtccta aaaaaattga
    27781 tttcatccgc aggctattga cagaataatt cagactggtc tttcaggcat ccagacacgc
    27841 taccgcccct ggctttttag ctaccaatac actgatttag cttaattttt caaaccctct
    27901 ctgcatacaa tcgttgatga gaaagggtta ttgcggaaat taacttccga atataaggtg
    27961 acattatggt aattgaatat tggctctcca ataatgcagg aggaagtgtt acagctaacg
    28021 gaatagcagg caaggtaaca attcggtaat tggctatttt taagaattat attaagtgtc
    28081 attcaatatg gtttttagga gtttctttag gttgacaata tttaatattg cgcctccaca
    28141 tgcgatattt cttaaataat gttttattat taccactttt aattcaggga taatgtgagg
    28201 ttattaccct caaataatat gagataaata gtgctgcaac attgcatttt tgccccgatt
    28261 tatccatgat cgaattatga catttgtcat acaacgaata ggttttgtac ttactatgga
    28321 atggattgcc gatccgtcta tctgggccgg gttaatcacg ctgattgtga tcgaactggt
    28381 cctcggcatt gataacctgg tctttattgc catcctcgcc gaaaaactac cgccgaagca
    28441 gcgcgaccgc gcacgggtta ccgggctgct gctggcgatg ttaatgcgcc tgttactgct
    28501 ggcgtcaatc tcctggctgg tcaccctgac tcaaccgctg ttcagcttct gctcgtttac
    28561 ctttagcgcc cgcgacttaa tcatgctgtt tggtggtttc ttcctgttgt tcaaagccac
    28621 gatggagctg aacgaacggc tggaagggaa agacagcaat aatcccacac aacgcaaagg
    28681 tgcgaagttc tggggcgtgg tgacgcaaat tgtggtactg gacgccatct tctcactcga
    28741 ctcagtgatt accgccgtcg ggatggtcga ccatttactg gtcatgatgg ccgccgtggt
    28801 tatcgctatc agcctgatgt tgatggccag caagccgtta acgcaatttg ttaacagtca
    28861 cccgaccatc gttattctct gcttaagctt cctgttgatg attggcttta gcctggtggc
    28921 agaaggtttc ggcttcgtca ttccgaaagg ctacctgtac gctgccattg gtttctcggt
    28981 gatgatcgag gcgctcaatc agttggctat ctttaaccgg cgacgttttc tttccgctaa
    29041 ccagacgctg cgccagcgga ctaccgaagc ggtaatgcgc ctgcttagcg ggcaaaaaga
    29101 agatgcggaa ctggacgccg aaaccgcgtc catgctggtg gatcatggta accagcagat
    29161 ctttaatccg caggaacggc ggatgattga gcgggtactt aatcttaacc agcgtactgt
    29221 cagcagcatt atgacgtcgc gccacgatat tgagcatatc gatctcaacg cgccggaaga
    29281 agaaatccgc cagttgctgg agcgaaatca gcatacgcgg ctggtggtta ccgacggtga
    29341 tgacgcagaa gatttgctcg gtgttgttca cgttatcgac ctgctacaac agtcactgcg
    29401 cggcgaaccg ctcaacctgc gggtgttgat tcgccagccg ctggtgttcc cggaaacctt
    29461 gccgttgtta cctgccctgg agcagttccg taatgcccgc acgcactttg cttttgtagt
    29521 ggatgagttt ggctcggtgg aagggattgt gacattaagt gacgtcactg aaaccattgc
    29581 cggtaactta ccgaacgaag tggaagagat cgacgcccgc catggtattc agaagaatgc
    29641 ggacggttcc tggacggcga atggtcatat gccgctggaa gatctggtgc aatatgtgcc
    29701 gctgccgctg gatgaaaaac gtgaatatca caccattgcc gggctgttga tggaatattt
    29761 acagcgtatt ccaaaacctg gcgaagaagt tcaggtgggg gattatttgc ttaaaacgtt
    29821 gcaggtggaa agccatcgcg tgcagaaggt gcagattatt ccgctgcata aggatggcga
    29881 gttggagtac gaggtgtgat ggtatcggtc attttacccg atagccgaaa ggaataagat
    29941 gagggaaccg cgccagcggc gatttaccgc cgggagcccg ggttgccagg gtggtggcga
    30001 ttgagccacc ctggcacgtt caccggcttg agcgttccat agtagtgagg aacatcaagt
    30061 gaacggaaca gccttcgatc ttacatattc agaatataat ggtgccgccg cgaacgcctg
    30121 atgcgacgct ttacgcgtct tatcaggcct acaggacagg tcacctggta acctacatct
    30181 tctccagcaa cttcttcaca tctttgccat tgcgggaatc tttattccgc tcggcccagt
    30241 cattcaggcg acgtttcgct tcgtcctgta gatgtttgcg cagtaactga tccacttgca
    30301 gactgtaatt gagttgctgc cagttgccat aaacccgcag cggtactggc gtttctttga
    30361 ggaaatcaat cagtttgctt tccccgttcc agccacccac gacccgaata tcaaactggg
    30421 tgtcgcaggt ttgatctgcc agattcaaca tgccttcccc tgtcagcgcc agcacaggcg
    30481 attgaccttg catgtcgttt aacgtcacga cgccatcctt caacgtcaaa tcggtggtaa
    30541 agcggtcaag acgcgttacg ttatcgaagt tttcagcggc cttcacatca ccaccattac
    30601 gttctaccgc ttgctgaatc atctgctgga agttcatccc ttccatgcgc gtgtcggtca
    30661 tttcgacatg tgcctgtcct tgccagttgt ggcggaatgc gtcggcatct atgtcagcac
    30721 cggagaaatc accagccagt gacatttttc cggtcaacga aatcggatag ttaaacgcct
    30781 tcagaatggt accaatctca acgttttcca gccgtggctg gaagtttatc cgcggattta
    30841 ttgatgtcgc gtccagcgtg cccggcagtg aaacctgtcc accgttaagc ttgccctgca
    30901 gttgagtaat ttccagcaaa cccgacttgt tggtcatttg cgtggcaaca tctgtaaaat
    30961 tcattccgcg ccagcgcacg ttactggcct gcaacaaaat atcagccgta aagccttgca
    31021 gtccctgata tgccggttca tcaatacgcg aagaaatgac cgggcgcggc aatgttgatt
    31081 ggctctgccc ctgctgcgcg gcaccgtttt caccattcgc tgtttcatta agcgggatga
    31141 ggttgtcaag attcagttgc gggaattgca gtctcagctg ccattcaggt ttctctgtca
    31201 gcgtgacctg tgcttgcccg ctcagcgtgc tgtcattggc ggtcaagctg atttggttaa
    31261 atgaaaggcg tttatgtgac tcctgccact gggcctggaa actcccctgc ccctgaatac
    31321 cttgttttgg taaatctgca ccctgcaact gccagttaat ttgttcaata gccgccgtta
    31381 aatcatgcgg ataatcagaa gcatctaccg taccgttaag ggatatcgtg agatcgcgct
    31441 gatcgcgatt aacccgcccg gagaactcaa atgagccacg atgttgggga tcttgttcca
    31501 tttgcaggcg gatattgcgg attgtcacct gctcgtcatc ttcatgctgg aacaccagca
    31561 cgctgtccgc caccttaaga ctggatatat caaacgacca tccgcgatcg tctgacagat
    31621 ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc actgcgcacc gcttccgtct
    31681 gcggcgtcag ttggatcact gcccctttta gcatcacctg cttaacgctc agttgatgac
    31741 tcagtagtgg taaaagcgcc acgtccagac gcatgttgtc ggcgcgaacc agtggctggc
    31801 ttgcgccctg ggcggtgaga gacattcgcc cggagaggat actaagctgc ggccagacgt
    31861 gccaacgcag tggcccgtcg agctgcaatt gataaccgct acgtgcagca acttgcttga
    31921 ccatatagtc gcggaaatca ttcggattca ccagcaacac taacgcagat aacccggcca
    31981 ccagcacgac caggagtatc atcagcgtcg tcagaaatcg tctcatggta tcctcaatgg
    32041 gcctgaatta gtctttatcg attcggctgg ctaccgcgcc ctgctggttg cgatatttcg
    32101 catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga aagcggctca aagctcagcg
    32161 caccaattaa catgcccgga cgcagcgcca gcggcagctt accggagttg tagaactcca
    32221 gcacaatgca accagaccag cccggatcga tacggtgcgc ggtgacgtgc accatcagcc
    32281 ccagacgcgc caaagaggaa cgcccgtcca gccagcccac cagatcggct ggcagcgtca
    32341 ccgactccag cgtcaccgcc agcgccagct ctcctgggtg aagatagaac gcctcgccct
    32401 cgtcgagaac gatctcatcg ctcatcacgc ggtcaagcgc ggcgctcact tcatctttgg
    32461 gaccgctcag atcgataaac gctgccgtgt gaccacggaa ggtacgaaat ttattgccca
    32521 ggcgtacatc caccgtcgcg ccgttaatac gctccactgg cggacgtggg ttgatcgaca
    32581 aacggccttc atcaagccag gcttcaatat ctcggtcaca cagacgcatg gcattttctc
    32641 ctttcgcgca tcactccctt aacgccaatt acgtcaaggg cataccaggt tatcactgaa
    32701 cggtacacaa tttatcaagc ttattcaaag aactgactta ttttcgcttt caatatatcg
    32761 atcgcgatgc ggtttttccc gccgcgcggc acgataatgt ccgcatattg tttagaaggc
    32821 tcaatgaatt gcaggaacat cgggcgcacg gttttttgat attgcgccat cacggaatcc
    32881 attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc gcatcaggca gatatccagc
    32941 ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgca aacgcgcatc cgtcagcaac
    33001 aaaatgcctt cgagaatgat gaccttcttc ggctcaaccg tcaccgtttc tttcatacgc
    33061 gtatgttcaa catagctgta aaccggcagg tcaattgccg agccgcgttt caacgcttgt
    33121 aaatgctcaa gcaacaggct gtgatccatc gcgctgggat ggtcgtagtt ggtcttaacg
    33181 cgttcttcca tcgacagatg gctttgatct ttgtaatagc agtcttcggg aattacgccg
    33241 atgtgttcat caccgacttg ctcacgtaat tcacgataaa gggtactggc aataagactc
    33301 ttgccggaag ccgatgcgcc agcgataccg ataatgacgc actgatgaga ctgatcagtc
    33361 atatatttag cgacctgatt aacctggatg ttaggaaggg ggcgacgaag cgccaaacgc
    33421 ggcaattata gggatttcat ccgcctgata ccagtcgaat agcgctgccg cgcgctcaga
    33481 gttaattgtt gacgaagaat tcccggtggc aaattacgtt gatcagtttt atacaaggta
    33541 aaaaatgtta tacgcagttg cgcaaattat ccgcctttac gtcactttat gagcaattcg
    33601 catataaaat gtaaaacttt tgtactagca taaacacaga aacgaatact ggcgacctgg
    33661 tcttgcggat aaagcggtaa tgagcaaaca atcacagcat gtattaattg ccctgcccca
    33721 cccgctgctt cacctggtca gtttaggttt agtctcgttt atctttaccc ttttctcgct
    33781 tgagctttcg cagtttggca cccaactcgc cccactgtgg ttcccgacgt ccatcatgat
    33841 ggtggcgttt tatcgccatg ccgggcgcat gtggccggga attgcgctga gctgctcgct
    33901 gggaaatatc gccgcatcca tcctgctttt ttccaccagc tcgctgaaca tgacctggac
    33961 gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg ctacgtaaat tgctgccgtg
    34021 gtataacccc ttgcaaaatc tggctgactg gctgcgtctg gcattcggca gcgccattgt
    34081 tccgcctctg ctagggggtg ttctggttgt cctgctgacg cccggagacg atcctctcag
    34141 ggcatttttg atatgggtac tgtcagaatc catcggcgct ctggcactgg tgccgctggg
    34201 attgttattt aaaccacact atctgctgcg ccatcgcaac ccacggttgc tttttgagtc
    34261 gctgctcacg ttagccatca cactgacgtt aagctggctt tcgatgctgt atctgccgtg
    34321 gccttttact ttcattattg tgctgttgat gtggagcgcc gtgcgcctgc cacgaatgga
    34381 agcctttttg atcttcctta ccacggtgat gatggtgtcg ctgatgatgg ccgcggatcc
    34441 ctccctgctt gctacgccgc gtacatacct gatgagccat atgccgtggc taccgttttt
    34501 gctgatcctg ctgcccgcca acatcatgac gatggtgatg tatgcctttc gtgcggaacg
    34561 caaacacatt tccgaaagcg aaacccgttt tcggaacgcc atggaatatt ccgctatcgg
    34621 tatggcgtta gtgggcaccg agggacaatg gctgcaaacc aacaaagcgc tctgccagtt
    34681 tctcgggtac agtcaggaag agctgcgcgg actcaccttt cagcaactga cctggccgga
    34741 ggatctcaat aaagatctcc aacaggttga aaagctgata agcggtgaaa taaacaccta
    34801 ttcaatggaa aaacgctact acaaccgcaa tggcgatgtt gtctgggcgt tgcttgccgt
    34861 ctcactggtg cgccacacgg atggcacgcc gctctatttt atcgctcaga ttgaagacat
    34921 taacgagcta aaacgcaccg aacaggtgaa tcaacaactg atggagcgca tcacgctggc
    34981 taacgaagcg ggcgggattg gcatctggga gtgggagttg aagccgaata tttttagctg
    35041 ggataagcgg atgttcgagc tgtatgaaat tcctccacat atcaaaccga actggcaggt
    35101 gtggtacgag tgcgtgctgc cggaagatcg ccagcatgcc gaaaaagtga ttcgttgcaa
    35161 tcacgctcgc cctttaaact ggaatttcgc attaccgtaa aagacggtat tcgccatatc
    35221 cgcgccctcg ccaaccgggt actgaataaa gaaggcgaag tcgaacgcct gctcggcatt
    35281 aatatggata tgactgaggt taaacagctt aacgaggcat tgtttcagga aaaagagcgc
    35341 ctgcacataa cgctggactc catcggcgaa gccgtggtct gtattgatat ggcaatgaaa
    35401 attaccttta tgaatccagt ggcggagaag atgagcggct ggacgcagga agaagcatta
    35461 ggtgttccgc tcctgacggt gttgcatatt acctttggcg acaatggtcc cctgatggag
    35521 aacatttaca gtgccgacac ctcacgttcc gcgattgaac aagatgtggt gttgcactgc
    35581 cggagcggcg gcagctacga cgtgcattac agtattacgc cgttaagtac tctggacggc
    35641 agcaatattg gttcggttct ggtgattcag gacgttaccg aatcacgcaa aatgctgcgc
    35701 cagctgagct acagcgcctc ccatgatgca ctgacgcatc tcgccaatcg cgccagtttt
    35761 gagaagcaac tgcgtatgct gctgcaaacg gtaaacagta cacatcagcg acatgccctg
    35821 gtgtttatcg atcttgatcg ctttaaagcg gtgaatgaca gcgccgggca tgcggcgggc
    35881 gacgctttgc tgcgcgaact ggcgtcgttg atgctgagta tgctgcgttc cagcgacgtg
    35941 ctggcgcgac tcggtgggga tgaatttggt ctgctgctac cagactgtaa tgttgaaagc
    36001 gcgcgtttta tcgctacacg cattatcagt gccgtgaatg actatcactt tatatgggaa
    36061 ggccgtgtgc atcgggtagg tgccagtgcc gggattacct tgattgatga caacaatcat
    36121 caggcggcag aagtgatgtc gcaggctgat atcgcctgtt atgcctccaa aaatggtgga
    36181 cggggccggg tgacggttta cgaaccacag caagctgccg cacatagcga acgggcggcg
    36241 atgtcgcttg atgaacagtg gcggatgatt aaagagaatc agttgatgat gatggcccac
    36301 ggtgtcgctt cgccgcggat cccggaagcg cgtaatttgt ggctgatttc gcttaaactc
    36361 tggagttgcg aaggcgagat tattgatgaa caaaaatttc gtcgtagctt cagcgatccg
    36421 gcacttagcc atgctcttga ccgacgggta ttccacgaat ttttccagct ggccgcaaaa
    36481 gcggttgcca gtaaaggcat aagcatcgcc ctcccccttt ccgttgccgg tttgagtagc
    36541 gccacgctgg tgaatgatct gcttgagcag ctggaaaata gccctctacc accacggtta
    36601 ttacatctga ttattccggc agacgcgatt ttagatcacg cagaaagcgt gcaaaaactg
    36661 cggctggcgg gatgtcggat agtgctcagc caggtgggcc gcgatctgca aatcttcaac
    36721 tcgctgaaag cgaatatggc agattacctg ctacttgatg gtgagttatg cgccaacgtg
    36781 cagggtaatt tgatggatga gatgctgatt acgattattc aggggcacgc tcagcgactc
    36841 gggatgaaaa ccatcgccgg gccagtcgtt ttacccttag tgatggatac gctttctggc
    36901 atcggcgtcg atctgattta tggtgatgtg attgccgatg cccaaccgct ggatttgctg
    36961 gtgaatagca gttatttcgc gattaactga gggagcgttt gcccattgcc tgatgcgacg
    37021 catcaggcct acaattcgtc cgttccgtct ggttgccagc cttccgtata ccagatatgc
    37081 aacagcgcat aagaacgcca gggcttccag cgctcggcat aacggcggat ttgtgccggt
    37141 gtcatccccg gaaatcgctg tttaatcaga taatcatccg gcagaaaaac atctttcgcc
    37201 tgccagccac gcaaagcgaa ataattcgcc gtccagcgcc cgatacccgg aaaagtttgc
    37261 agcgttttca tcgcctgctc cacatcgccc ggaattgtca ttggtaagct gccctccagc
    37321 gccgcatttg ccagatgaat cagtgcctct gcccgtttca acggcatgcc taacgctttt
    37381 aatgcctgcg ggtcggctgc tgccagccgc tgaggcgtgg ggaagcagat atactccgga
    37441 aaatcatcca gccgttcgcc ataaagctgt acaactttgg cagtcaattt tgccgccatc
    37501 gccacgctca ccagttggcc taaaatcgcc cgcacaccct gctcaaaagc atcaatacag
    37561 ccgggtaaac gcaatccggg ccgcgccgcg cctaacttgc ccaacgcacc gttaacaatc
    37621 tgcgggttac attgcagatc aaacaggcgg ctcattttcg ccagacactc agcggcaacg
    37681 ggttctaaac ctgcacttaa atttatgtgc agagtatggc gggctatatc cggaatagca
    37741 gtcaccacgc cgcgatattc gcccaccgcc agactacggg cataataatc ctcagcgacc
    37801 gtttccacac cactcactgc acgggcggcg agaaatccca acatccacga ccagtcatac
    37861 ggcggctgcc agttcagggt atacatcgca tctccttatt catccgcttt cagcataaac
    37921 gttattcaga cgctacgctt tgctttcata ttccggttgt cgcgacagca acatttcgct
    37981 aaagtcacgc cccttcttca ccggcatggg gattaattcg tgtttattgg ttttgattac
    38041 ggtacagcaa actgttcagt ggcggtcatg cgtgacggta aaccgcaatt actgaaaatg
    38101 gaaaacgaca gcacgctgct gccttcaatg ctttgcgcgc caacgcgtga atcggtaagc
    38161 gaatggctgt accgccatca tgatgttccg gcagacgacg atgaaacgca ggcgctgtta
    38221 cgtcgggcga ttcgttataa ccgcgaagaa gatatcgatg ttacggcgaa aagcgtgcag
    38281 ttcggtcttt cctcactggc acagtacatt gatgatccag aagaagtgtg gtttgtgaaa
    38341 tcaccaaaat cgttcctcgg tgccagcggc ttaaaaccgc agcaggtagc gctgtttgag
    38401 gatctggtct gcgcaatgat gttgcacatt cgccagcagg cgcaggcgca gctgccagaa
    38461 gcgattactc aggcggtgat tggtcgcccg atcaacttcc aggggctggg cggcgatgaa
    38521 gcaaacgccc aggcgcaagg gattctggaa cgcgcggcga agcgtgccgg attcagggac
    38581 gtggtattcc agtacgagcc ggtcgcggct gggctggatt acgaagccac cttgcaggaa
    38641 gaaaaacggg tgctggtggt ggatatcggc ggtggtacga ctgactgttc attgctgctg
    38701 atggggccgc agtggcgttc gcgtctcgat cgtgaagcca gcctgctggg tcacagtggt
    38761 tgccgtattg gcggtaacga tctggacatc gcactggcgt ttaaaaacct gatgccgtta
    38821 ctgggcatgg gtggcgaaac cgaaaaaggc atcgccctgc cgattctgcc gtggtggaat
    38881 gcggttgcca tcaacgacgt acctgcgcag agtgatttct acagtagtgc caacggtcgt
    38941 ctgcttaacg atctggtacg cgatgcccgc gaaccggaaa aagtggccct gctacagaaa
    39001 gtctggcgtg agcgtttaag ctatcgcctg gtgcgtagcg cagaagagtg caaaattgct
    39061 ctttcaagcg tagcggaaac ccgcgcctca ctgccgttta tcagcgatga actggctacg
    39121 ctgattagcc agcaagggct ggaaagcgcc ctcagccagc cactggcgcg gattctggaa
    39181 caggtgcaac tggccctgga taacgcccag gaaaaaccgg acgttatcta cctgaccggc
    39241 ggtagcgccc gttcgccgct gattaaaaaa gcgctggcag aacagttgcc gggaattccg
    39301 attgcaggcg gcgatgactt tggctccgtc accgccgggc tggcacgctg ggcggaagtg
    39361 gtgtttcgtt aaaagaaaat ggctcagcag cggcttaaat ctgcctacgc ctgagccagt
    39421 ttttctgccg cctgacgtaa aggttccatc aacggcatgg tccgtgctgg agcatgaagg
    39481 cggaatcgct gcaactctgt cgcgccgaca gtcaacgtcg attccagccg atgtcgtttg
    39541 gcggtcattt tcgccattac cgcttgtctg tctgctggcg ttatcgcttc attcggcctg
    39601 aacacaaaac ggcgttcaca gctcgctttc cagtcgataa ccgcctgggt cagatggtcg
    39661 ccaaaccctt tcacttgctt aacgccacga cgggtaacat ctgcggctgt ttcaatacca
    39721 aaagaccgta acgccgcttt acgcgcaggg ccaacgccgg gaatagaggc aacatcaata
    39781 aaaaatccct ccagaaactt ctgcttctgc cgttcccttg cagtgtcatg aagtgctgcc
    39841 agagcgcgtt tctcttcttc cggtaacccg agaatttcgt ccttcatttt ttcgagcatc
    39901 gcccgtttgg cgataaaacc ttccagcccg cccaactgtt ggatctggct gactaaatgc
    39961 tcatagtcca ttttcgcgcg attaaatgcc tcacggcgtt gctggatttc tgctttgtac
    40021 gctttgcttg tcagactgcc aataatccag atagccgaca gcacaggaac caaaataata
    40081 taacgcggtt ctgcctgaag gccgcaaagc aacagcgata acgctgacag tgcgatctca
    40141 atcagaatga tgtattcgcg ccgtaacagg cctaaaggaa gcggcctgcc agtcggttgg
    40201 aaatgatcgg gtaatggcaa ttgcaatgct ggcggtgcta ctgacgccat caccatcgcc
    40261 cagactttcg ccagcacaaa atcaccgccg gtggtaatga cctcttcgcc gagatcaata
    40321 aaatagataa cgccttgatt atccagcgca caccacgggc agtcggtcaa atgagcggga
    40381 taaacatgca ttgccgaaac gggacatttc tttaactgtt ggcgcagaga atcaagtgcc
    40441 gctacccacg ctttagccgt cggacgcccg gttgccacgc cactttccgt gaatgcctgc
    40501 tgaaacatgg cttcaacatc gcccggtaac atcgatagcg gaatcgatcg tggcggcggt
    40561 tttaaaccac gtcgctgatt atctgacgcg taggcataac ggaaatgggt aatatccgtc
    40621 tccagcgcat tacccgcatc agagataagc ggcacaccag aataaggatg ccggccacca
    40681 aacaagacgt gaaaaatcag caacgcaagg ccaaaattat cgtgattttc tgtgcgttca
    40741 aagccgacaa atgacgacaa ggtttgtagc tctggcggcg taaaatgcga cacgccgact
    40801 tcgcataaat gcagtgtgcc attggcgtta atctgaaagg agtcgctatc aatcagtacc
    40861 actttgctgt cgcgacctac cataaaactg ttctggttta cgtcacccac gacgtgcccg
    40921 tgctcgtgaa ccgtagcaaa agatgaagca atattgcgcg caacatagag tagaaaatcc
    40981 cacgcacaat gagggtaacg ttgacgacga tgtgccgggc tatagatcat atgaatcggt
    41041 tctttaccag aaacttttgg catcataaaa ccgataactt ttccgcctcg tccaccgtga
    41101 agcgttgcct gcggccaggc gacataattc aacaattgcg cgtcagctgt cgcagccata
    41161 aaggcaagtt tgtcctgttt taaggcgggt ggcggcgtgt gataaatctt ggcgacgcta
    41221 tcgacaaact cattgatatc ataaaccgcg ccttcaccgc ctttgcccag ttcacggcct
    41281 ggcgtaacgc actcaccagt agcagtatat aaagtgggtt tcatggtaac cacagcgcca
    41341 gtgccaacgt tttatcatca tccgtacgct cgtttaccgc cgggctggac aaaaactgct
    41401 ttaacaattc aggtaataaa tcgagttgtt cctgcgttgc tgacgccagt ccattgaaaa
    41461 acggggtaaa gaaaggtaca tgaggagaat tatccagcat atttaacgcc agtcgctgga
    41521 taccatccgt aaatgctgca actttatgca cacgctcagt gctggtgaaa gtttccagtc
    41581 tggaaacggc atcttcatcg gtaatgaagt gcgtcatatt ggcatattca cccaccatcg
    41641 gcgtaagcgg caattgcaga ccgtgaccaa aatccaccac cacgccgcca tcgccaatct
    41701 gcataatcaa ggtgccatta gccgatgaaa tcaatcccag aaacgtgcag gcaaaatcgc
    41761 gaactgctaa ttctttggct tccgcctcgg caaaaagtcg ctgacgaacg gtcagcacaa
    41821 tatccgtggc gaggatatca ttaagcccca attccccacc ctgcactttt tgcgacatat
    41881 aagccatcgc ttcattgacg gcgagcgtcg cgccttctcc tccctgcgaa acgctacctg
    41941 cgccatcagc aaggaacatc accagtaatg gctgctggtc atttagccag gcaacctgca
    42001 tctggcaggc atcctggcaa ggcaagtccg cgctgatgtg cgatgttcca acagcggatg
    42061 cataaaccag acgccagctc acactgacgt ccagcctttt ggcgcttcca gcacaacttc
    42121 cgttcccggc gtggagcggg aaaccgaacg cagcgaactg gataaccagc taaacagttc
    42181 acggaattgt aatccttgca gaggcaaagg ctgacgaacg ctgatttgtg ccagagtctt
    42241 catatccgcc ccctgaacac cgatggaaaa gaaagcaaac cttttatctt cttccccccg
    42301 aaacactttg ttggctgcag cctgccactc atcggttggt gcaccatcgg taatcaggaa
    42361 aatccacggg cgataatacg aaataccatt tgcacgatac tcacgtttcc gctcctctac
    42421 catatccagg gctttggtaa tggccgcacc cattggtgta tcgccctggg caaacaggat
    42481 gggcggaaaa aaattagcgg cgctggtaaa gggttgttcc acatgcaccg ggccgaacgt
    42541 cacaatgccg agttccactc tttttaaagc cagcggatcg gcaagcagtt catcacgaaa
    42601 ggtcaccaat ccggcgttaa gttcattaat aggtctgccg ctcatcgagc cagaaacatc
    42661 cagcagcaaa atgcaggggc aacgtggttc cgggttactg gcaaaatcgc tggtggcaaa
    42721 cgtgatttgt tcgctcataa gaaatccttt cttagagaat tatcgaggtc cggtatcata
    42781 ccgactgcaa taatgaaata agagcaaacg aagaaacttt cagaattagg cccggcggcg
    42841 cagaaaattt tgctattcgc ctcgcaagaa cggagagccg tcgattgaca ctaagggcgg
    42901 agtgacataa tttcaggagt gagggttagg gagaggtttc cccctccccc tggtgttctt
    42961 agtaagcctg gaagctaatc actaagagta tcaccagtat gatgacgtgc ttcatcataa
    43021 ccctttcctt attaaaagcc ctcttctccg ggagaggctt tcccgtttca gcgtcccgct
    43081 gaaatcatcg gcttacctcc tttcgccctg aatgcagtct atcgctaacg cgtgaataaa
    43141 atagtttcct ctgtgattat tgcgtaacga ctcgcaaaga atatttgctg gcaggatcgc
    43201 agactacaaa gcctgcggat tgacaatctt atcgtgaagg catactttca ggagtgaggg
    43261 tagagcgggg tttcccccgc cctggtagtc ttagtaagcg gggaagctta tgactaagag
    43321 caccacgatg atgagtagct tcatcatgac cctttcctta tttaaggccc cttcctcggg
    43381 aggggctttc ccgtttcagc gtcccgctga aatcgtcggc ttacctcctt tcgccatgca
    43441 cttagtctat cgctgacttg cagataaaat agtttccagt gtgattattg cgtaacgtct
    43501 cgcaaagaat ttttgctggc aggatcgcag acggcaaagc ctgcggattg acaatcttat
    43561 cgtgaaggca tactttcagg agtgagggta gagcggggtt tcccccgccc tggtagtctt
    43621 agtatgctgg gtagctaatc actaagagca ccacgatgat gagtagcttc atcatgaccc
    43681 tttccttatt taaggcccct tcctcgggag gggctttccc gtttcagcat ccggctgaaa
    43741 tctgcggctt acctcctttc gccctgtatg cagtctatcg ctaacgcgta gataaaatag
    43801 tttcctgtgt tattactgga tgcgtgctcg caaatgtgcc cgccattcag acgattcccg
    43861 atagtgtttc ataattcctc catttttctc ccttattggc tggctacact agtatcattc
    43921 cgcgaaacgt ttcaggaaga gaaactctta acgatgaaag gcagttataa atcccgttgg
    43981 gtaatcgtaa tcgtggtggt tatcgccgcc atcgccgcat tctggttctg gcaaggccgc
    44041 aatgactccc ggagtgcagc cccaggggcg acgaaacaag cgcagcaatc gccagcgggt
    44101 ggtcgccgtg gtatgcgttc cggcccatta gccccggttc aggcggcgac cgccgtagaa
    44161 caggcagttc cgcgttacct caccgggctt ggcaccatta tcgccgctaa tactgttacg
    44221 gtgcgcagcc gcgtggacgg tcaactgatg gcgttacatt tccaggaagg ccagcaggtc
    44281 aaagcaggcg atttactggc agaaattgac cccagccagt tcaaagttgc attagcacaa
    44341 acccagggcc aactggcaaa agataaagcc acgcttgcta acgcccgccg tgatctggcg
    44401 cgttatcagc aactggcaaa aaccaatctc gtctcccgtc aggagctgga tgcccaacag
    44461 gcgctggtca gtgaaaccga aggcaccatt aaggctgatg aagcaagcgt cgccagcgcg
    44521 cagttgcaac tcgactggag ccgcatcacc gcaccagtcg atggtcgcgt tggtctcaag
    44581 caggttgatg ttggtaacca aatctccagt ggtgatacca ccggaattgt ggtgatcacc
    44641 cagacgcatc ctatcgattt agtctttacc ctgccggaaa gcgatatcgc tactgtagtg
    44701 caggcgcaaa aagccggaaa accgctggtg gtagaagcct gggatcgcac caattcgaag
    44761 aaattaagtg aaggcacgct gttaagcctc gataaccaaa tcgatgccac aaccggtacg
    44821 attaaagtga aagcacgctt taataatcag gatgatgcgc tgtttcccaa tcagtttgtt
    44881 aacgcgcgca tgttagtcga caccgaacaa aacgccgtag tgatcccaac agccgccctg
    44941 caaatgggca atgaaggcca ttttgtctgg gtgctgaata gcgaaaacaa ggtcagcaaa
    45001 catctggtga cgccgggcat tcaggacagt cagaaagtgg tgatccgtgc aggtatttct
    45061 gcgggcgatc gcgtggtgac agacggcatt gatcgcctga ccgaaggggc gaaagtggaa
    45121 gtggtggaag cccagagcgc caccactccg gaagagaaag ccaccagccg cgaatacgcg
    45181 aaaaaaggag cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc
    45241 gcctgtttat tatgcgtcct gtctccacca cgctgctgat ggtggcgatc ttactcgccg
    45301 ggattatcgg ttatcgcgcc ctgcccgttt cggcgctgcc ggaagtggac tatccgacca
    45361 ttcaggtggt cacgctctac ccaggtgcca gcccggatgt catgacctct gccgttaccg
    45421 cgccgctcga acgccagttc gggcagatgt ctggcctgaa acagatgtcg tcgcaaagtt
    45481 ccggcggtgc gtcagttatc actttgcagt tccagctaac attaccgctc gatgtcgccg
    45541 agcaggaagt gcaggccgcg attaacgctg cgaccaactt gttgccgagc gatctgccta
    45601 acccgccggt ttacagcaaa gtgaacccgg cagatccgcc gatcatgacg ctcgccgtca
    45661 cctcaaccgc catgccgatg acgcaagtgg aagatatggt ggaaacccgc gtcgcgcaga
    45721 aaatctcgca gatttccggc gtcggcctgg tgacgctttc cggcggtcag cgtccggctg
    45781 ttcgcgtcaa acttaacgct caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc
    45841 gcaccgccat taccggcgct aacgttaact cggcaaaagg tagcctcgac ggcccttccc
    45901 gtgcggtcac gctttccgcg aacgaccaga tgcaatccgc cgaagagtat cgccagctaa
    45961 tcatcgccta ccagaacggc gcgccaattc gtctgggcga tgtcgcaact gtagagcaag
    46021 gtgcagaaaa cagctggctc ggcgcgtggg cgaacaaaga acaggccatt gtgatgaatg
    46081 ttcagcgcca gcccggtgct aacattatct ccaccgccga cagcattcgg cagatgctgc
    46141 cacagctcac tgagagtctg ccgaaatcgg tgaaggtgac agtgctttcc gatcgcacca
    46201 ccaatatccg cgcatccgtc gatgatactc agtttgaatt gatgatggct atcgcgctgg
    46261 tagtcatgat tatctacctg tttttgcgca atattccggc gaccatcatt cccggtgttg
    46321 ctgtaccgct gtcgttaatc ggcactttcg cggttatggt gtttctcgat ttttcaatca
    46381 ataacctgac actgatggcg ttaactatcg ccaccggatt cgtggtcgat gacgccatcg
    46441 tggtgatcga aaacatttcc cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc
    46501 tcaagggcgc aggtgaaatc ggctttacca ttatctcgct gaccttctca ctgattgcgg
    46561 tgttgatccc actgctgttt atgggcgata tcgtcgggcg actgttccgc gaatttgcta
    46621 ttacgctggc ggtagcgatt ttgatctcag cggtggtgtc gctgaccctg acaccgatga
    46681 tgtgcgcgcg gatgctcagc caggagtcgt tgcgtaaaca gaaccgcttc tcccgtgcct
    46741 cggaaaaaat gttcgacagg ataatcgcct cctatggtcg tggactggcg aaggtgctga
    46801 atcatccgtg gctgacctta agcgtggcgc tcagcacgct gctgcttagc gttctcttat
    46861 gggtgttcat tccgaaaggt ttcttcccgg tacaggacaa cggcattatt cagggcactt
    46921 tgcaggcacc gcaatccagc tcctttgcca atatggccca gcgacaacgc caggtcgcgg
    46981 acgtgatttt gcaggatccg gcagtgcaaa gcctgacctc atttgttggc gttgatggca
    47041 ctaacccgtc gctgaatagt gcacgtttac aaatcaacct caaaccgctg gatgaacgtg
    47101 atgaccgagt gcaaaaagtc attgcccgtc tgcaaacggc ggtggataaa gtgccgggcg
    47161 tcgatctctt cctgcaacca acgcaggatc tgactattga tactcaggtc agccgcaccc
    47221 agtaccagtt taccttgcag gccacctcac tggatgcgct cagtacctgg gtgccacagt
    47281 tgatggaaaa actccagcaa ctgccacagc tttctgatgt ctccagcgac tggcaggaca
    47341 aagggctggt ggcgtatgtc aatgttgatc gcgacagcgc cagccgtctg gggatcagca
    47401 tggcggatgt cgataacgcc ctgtacaacg cgtttggtca gcgactgatt tccactattt
    47461 atactcaggc taaccagtat cgcgtagtgc tggagcacaa caccgaaatt accccgggcc
    47521 tcgcggcgct ggataccatt cgcctgacca gtagcgacgg cggcgtggtg ccgttaagct
    47581 caattgccaa agttgaacag cgttttgcgc cgctttccat caaccatctg gatcagttcc
    47641 cggtaacgac catctccttt aacgtgccgg ataactattc gctgggcgat gcggtgcagg
    47701 cgattatgga caccgaaaag acgctgaatc tgccggtgga tatcaccacg caatttcagg
    47761 gcagcaccct cgccttccag tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg
    47821 tggtggcgat gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca
    47881 ttctctcgac gctacccacc gcaggggtcg gcgcgctgct ggcattaatg attgccggta
    47941 gcgaactgga tgtgattgcg attatcggca ttattttgct gatcggtatt gtgaagaaga
    48001 acgccatcat gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc
    48061 gcgatgcaat ctaccaggct tgtctgttgc gttttcgtcc gatcctgatg accaccctgg
    48121 cggctctgct tggcgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc
    48181 gtccgttagg tatcggcatg gtcggcggtc tgattgtcag ccaggtgctg acgctgttca
    48241 ccacgccggt gatttatctg ctgttcgacc gcctggcatt gtggaccaaa agtcgctttg
    48301 ctcgccatga agaggaggcg taagtgaagt tttttgccct cttcatttac cgcccggtgg
    48361 cgacgatttt actgtcggtc gccattaccc tgtgcggcat actgggcttc cgtatgctgc
    48421 cggtcgcccc gctgccgcag gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg
    48481 gtgcgtcacc agaaacaatg gcgtcttccg ttgccacgcc gctggagcgc tcacttgggc
    48541 gcattgccgg agtcagtgaa atgacctcca gcagttcgct cggcagcacg cgtattattt
    48601 tgcagtttga ttttgaccgg gatatcaacg gcgcggcgcg cgatgtgcag gcggcgatca
    48661 acgctgcaca aagtttgctg cccagcggga tgcccagccg tccgacctat cgcaaagcta
    48721 acccgtcgga tgcgccaatt atgatcctca cgctgacatc cgatacttat tcgcagggtg
    48781 aactgtacga ttttgcctcg acgcagctgg ctccgacgat ttcgcaaatc gacggtgtcg
    48841 gtgatgtcga tgttggcggc agctcgctgc ccgccgtacg cgtcgggctg aatccgcagg
    48901 cgctgtttaa tcagggcgtc tcgctggacg acgtacgcac cgccatcagc aatgccaacg
    48961 tgcgtaaacc gcagggggcg ctggaagatg gcactcaccg ctggcagatc cagaccaatg
    49021 atgagctaaa aaccgccgcc gaatatcagc cgttgattat tcattacaac aacggcggcg
    49081 cggttcgtct gggcgatgtg gcgacggtga ccgactcggt gcaggatgtg cgcaacgccg
    49141 ggatgaccaa cgccaaaccg gctattttgc tgatgatccg caagctgccg gaagccaata
    49201 ttatccagac ggtagacagc atccgggcaa aattaccgga gttgcaggaa accattccgg
    49261 cggcgattga tctgcaaatt gctcaggatc gctcccccac cattcgcgcc tcgctggaag
    49321 aagtcgagca aacgctgatt atctcggtgg cgctggtgat tctggtggtg tttttattcc
    49381 tgcgctcggg tcgcgccact attattcccg ccgttgcggt gccggtttcg ctgattggta
    49441 cgtttgcggc gatgtacctg tgcggattca gtctcaataa cctttcgtta atggcgctca
    49501 ccatcgctac tggtttcgtg gtggatgacg ccatcgtggt gctggaaaac attgcccgtc
    49561 atctggaagc ggggatgaaa ccgctacagg ccgcgctaca aggtactcgc gaagtcggct
    49621 ttacggtgct atcgatgagt ctgtcactgg tggcggtgtt cctgccgctg ctgttgatgg
    49681 gcggattgcc gggccgactg ttacgcgaat ttgccgtgac gctttctgtc gccattgtta
    49741 tatcgttgct ggtttctctg acattaacgc caatgatgtg tggctggatg ctgaaagcca
    49801 gcaagccgcg tgagcaaaag cgactgcgtg gttttggccg catgttggta gccctgcaac
    49861 aaggctacgg caagtcgctg aaatgggtgc tcaatcatac ccgtctggtg ggtgtggtgc
    49921 tgcttggcac cattgcgctg aatatctggc tgtatatctc gatcccgaaa accttcttcc
    49981 cggagcagga caccggcgtg ctgatgggcg ggatacaggc ggatcagagc atttcgtttc
    50041 aggcgatgcg cggcaaattg caggatttca tgaaaattat ccgtgacgat ccggcagtgg
    50101 ataatgtcac cggctttacc ggcggttcac gagttaacag tgggatgatg tttatcaccc
    50161 tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt ctgcgcgtaa
    50221 aactggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaggat attcgcgttg
    50281 gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac ctggcggcac
    50341 tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa ctggcggacg
    50401 tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac cgtgacacca
    50461 tggcacggct gggaatcgac gtacaagccg ccaacagtct gttaaataac gccttcggtc
    50521 agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg atggaagtgg
    50581 atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc aataacgaag
    50641 gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc ccactatcgg
    50701 tgaatcatca gggattatcg gcggcctcga ccatttcgtt caacctgccg accggaaaat
    50761 cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt gtgccttcga
    50821 cggtgcgcgg cagttttgcc ggaacagcgc aggtgttcca ggagacgatg aactcacagg
    50881 tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga
    50941 gttatgtgca cccgctgacg attctctcca ccctgccctc ggcgggtgtt ggagcgctgc
    51001 tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg atcatgctat
    51061 taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt gaagcccaac
    51121 ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc
    51181 cgattatgat gactaccctg gcggcgctgt ttggcgcgct gccgctggtg ttgtcgggcg
    51241 gcgacggctc ggagctgcgg caacccctgg ggatcaccat tgtcggcgga ctggtaatga
    51301 gccagctcct tacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctgcggc
    51361 tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat cttcccgaca
    51421 gcacccgttg gcaattgtgg attgtggctt tcggcttctt tatgcagtcg ctggacacca
    51481 ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata
    51541 tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc gccagcggct
    51601 ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg ctgtttactc
    51661 tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt
    51721 tacagggcgt tggcggcgcg atgatggtgc cggtcggcag attgacggtg atgaaaatcg
    51781 taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt caggtcggtc
    51841 cgctgctcgg tccggcgctc ggcggtctgc tggtggagta cgcgtcgtgg cactggatct
    51901 ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg ttaatgccga
    51961 actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg gcggttggca
    52021 tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg ccgctggcga
    52081 ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg cacgccagaa
    52141 ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc
    52201 tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg acaccggttt
    52261 tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg atcccgatgg
    52321 tgcttggcag catgggaatg aagcgaattg tggttcaggt ggtgaatcgc tttggttatc
    52381 gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg tttatgacta
    52441 ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa ggaatggtca
    52501 actcaacgcg tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaatctgg
    52561 cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca
    52621 ccatcgccgg gctgttgctg ggactttttg gttcgcagca tgtcagcgtc gacagcggca
    52681 ccacacaaac cgtctttatg tacacctggc ttagcatggc gttgatcatc gcccttccgg
    52741 cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa
    52801 aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt ttctggcgat
    52861 tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta tcagttttga
    52921 gcgtggcttt attgattaca tcaagcatgg taatgaacaa cgattacaac tgttaagtga
    52981 tgcgcttggc gagcagtatg cgcagcatgg caactggcgc ttcctgcgca acaatgatcg
    53041 ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata aacccggccc
    53101 gggtatgcca ccgcacggct ggcgtaccca gttctgggtg gttgatcaaa acaacaaagt
    53161 gctggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca ttctggtcaa
    53221 cggtgcggaa gttggcgcgg tgatcgcctc ccccgttgag cggttaacgc gcaatactga
    53281 tatcaatttc gataaacaac agcggcaaac cagctggctg atcgtcgccc tggcaacgtt
    53341 actcgcggcg ctcgccacct ttctgctggc gcgcggtttg ctggcaccgg taaaacgact
    53401 tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa cgcccaccag
    53461 tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag cttgccagca cgctggagaa
    53521 aaaccagcaa atgcggcgcg attttatggc cgatatttca cacgaactgc gtacgccatt
    53581 agcggtgctg cgcggtgaac tggaagccat tcaagatggc gtgcgcaaat tcacgccgga
    53641 gacggtggcg tctttacagg cggaagtcgg tacactgact aaactggttg acgatcttca
    53701 tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg tagatttgat
    53761 cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga tttgccagcc gtggcctgaa
    53821 actgcagttt tccctgccag acagtattac cgtatttggc gatcgcgacc gtttaatgca
    53881 gttattcaat aacttactcg aaaacagcct gcgctacact gatagcggcg gcagcctgaa
    53941 aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg
    54001 tgtcagtgac gagcagctac aaaaattatt tgaacgtttt tatcgcaccg aaggttcccg
    54061 caaccgcgcc agcggtggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc
    54121 acataatggc cgcattattg ctgcccattc gccttttggc ggggtaagca ttacagtaga
    54181 gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac
    54241 acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct cattgattat
    54301 ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt actgccgtat
    54361 gtgcgccaga caccaccgga tctgatcctg ttagatctga tgctccctgg caccgatggc
    54421 ctgacgctgt gccgggaaat tcgtcgtttt tctgacattc cgatcgtgat ggtgacggca
    54481 aaaatcgaag agatcgatcg cctgctgggg ctggagattg gcgcagatga ttatatctgt
    54541 aagccgtaca gcccacggga agtggtagcg cgcgtcaaaa ccattttgcg ccgttgcaaa
    54601 ccgcagcgcg agttgcagca acaggatgct gaaagcccgt tgattatcga cgaaggtcgt
    54661 tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt tcgtctgctg
    54721 aaaacgctct ctcatgaacc aggaaaagtg ttctcccgcg agcatttgct caatcatctt
    54781 tatgacgact accgcgtagt aaccgaccgc accatcgaca gccacattaa aaacctgcgc
    54841 cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta cggcgttggt
    54901 taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatga ctttgttagc
    54961 cggagatggc gttcagacca gatccggcaa cattatccca cgcatggtca gcaaactgac
    55021 attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatcg gtaaacagca
    55081 cctttagatg ctgttttcga tacgcaattt caatcaagga gtcattatgg ctggttggtt
    55141 tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg gcaatggtga
    55201 gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg gcatcgcgtc
    55261 ggtgcgtagc aacagcccgc aagaagaacg ctatgagaaa aaaacggcaa gtaacggcaa
    55321 attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc agatgtacgc
    55381 caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca caagccagac
    55441 ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc ggctttatat
    55501 ccaaataacg cttagttatt aataattaag agtaaccgcc gccattaaaa tagtgggcac
    55561 tgacgagcac tcgttgtgct ttctttgttt accatcccat acttatcctc ctgaacatgt
    55621 tcatgtgcca aaatatcttc taatactttc gtgacgctat ccggtttatg acatacactg
    55681 atgtaagatt tttacttaca ggcgggcgta aaatggccag aacgatgacg gttgatctcg
    55741 gcgatgagtt gcgtgaattt atcgagtcct taattgaatc cggcgactat cgcacccaaa
    55801 gcgaagtgat ccgtgagtca ctgcgattac ttcgagaaaa gcaagcagag tctcgcctgc
    55861 aagcgttacg tgacatgctc gcagaagggt tgagtagtgg tgaagctcag ccgtgggaaa
    55921 aggacgcgtt cttacggaag gtgaaagcag ggataaggaa ataatgtgcg tattattaaa
    55981 ctcatgccaa aagccaatga ggatttagaa ggcatctggt attacagcta tcaccatttc
    56041 ggcgaaccac aagccgacag atatgtggaa catctttcag acgttctcca gattctgagt
    56101 aacaataata taggtacgcc cagacctgaa ttaggcgaag gtatttttgt tcttcctttt
    56161 gaacgccacg tcatttattt cctgcaatca ccaggggaaa ttatcgtcat tcgtatcctt
    56221 aaccaaaacc aggacgccac cagacattta cactggagct aaacccgccg attgcgctac
    56281 aatgcccgcc cttaaagtgg gggcactccc ctaaccgctt catcaggtga agcggatctg
    56341 acctgtcatc agaacgagag aattatgttt aaaccggaac tcctttcccc ggcgggaacg
    56401 ctgaaaaata tgcgttacgc tttcgcttat ggcgcagatg ctgtttatgc gggccagccg
    56461 cgttattccc tgcgtgtgcg caacaacgaa ttcaaccacg aaaatcttca gctcggcatc
    56521 aatgaagccc acgcgctggg gaaaaagttt tatgtcgtgg tcaacattgc accgcacaac
    56581 gccaagctga aaacctttat ccgtgacctg aaaccggtgg tggaaatggg gccggatgcg
    56641 ctgattatgt ccgatccagg gctgattatg ctggtgcgtg agcacttccc tgaaatgccg
    56701 atccaccttt cggtgcaggc taacgccgtg aactgggcga cggtgaaatt ctggcagcaa
    56761 atgggcctga cccgcgtgat cctctctcgc gagctgtcgc tggaagagat tgaagagatc
    56821 cgcaatcagg tgccggatat ggagatcgag atcttcgttc acggcgcgct gtgcatggcc
    56881 tactccggtc gctgcctgct ctctggctat atcaacaagc gcgacccgaa ccagggcacc
    56941 tgcaccaacg cctgccgctg ggagtacaac gtccaggaag ggaaagaaga tgatgttggc
    57001 aacatcgtac acaagtacga gccgattccg gtgcaaaatg ttgagccgac gctgggtatc
    57061 ggcgcaccaa ccgacaaagt gtttatgatc gaagaggccc agcgtccggg cgagtatatg
    57121 accgcgtttg aagatgagca cggcacttac atcatgaact cgaaagatct gcgcgccatc
    57181 gcccatgtag aacgcctgac caaaatgggc gtgcattcgc tgaaaatcga aggtcgtacc
    57241 aaatctttct actattgtgc acgcaccgca caggtttacc gcaaagctat cgatgacgcc
    57301 gctgcgggaa aaccgttcga taccagcctg ctggaaactc tggaaggtct ggcgcatcgt
    57361 ggctataccg aaggtttcct gcgtcgtcat actcacgacg attatcagaa ctacgaatac
    57421 ggttattcag tttctgaccg ccagcagttt gttggtgagt ttaccggtga gcgcaagggg
    57481 gacctcgcgg cggtagcggt gaaaaataaa ttctccgttg gcgacagcct tgagctgatg
    57541 acgccgcaag gcaacattaa ttttaccctt gagcacatgg aaaacgccaa aggcgaagct
    57601 atgccgatag caccaggcga tggttatact gtgtggctcc cggtcccgca ggatcttgag
    57661 ctcaattacg cgctgctgat gcgtaatttc tccggggaaa ccacgcgtaa tccccacggt
    57721 aagtgattaa tttcgattat ttttcccgga tggaaaattc ttagaaaccg atcacataca
    57781 gctgcattta ttaaggttat catccgtttc gctgaaaaac ataacccata aaatgctagc
    57841 tgtaccagga accacctcct tagcctgtgt aatctccctt acacgggctt attttttatg
    57901 cataagccct atccctggtc accgtcttcc attgaccaca tcgatagaat ctcccttcat
    57961 agcacgatgc ctttcacgta acggcatcgt gctcgcacag gttccggcta agcacaacca
    58021 gaacgcgcat gtttgacgct taccaaaaaa tattctcact ctccacattt gaatgtcaga
    58081 cgagcgacgc catgtaatcc tgcaccttct gtcttcaggt caactatctg catttttttg
    58141 ccctgagtaa cacagaaatg ggctgcatca ttttttacta tattttctgc accagatatt
    58201 ctgcccctgg ctaaagaagc ttcggcttcg gtgtagtatt ggttatcgag tttacgctga
    58261 atattacttt tatatgcaag accaaattta ccgatacttg tctcatcatt atgcacagca
    58321 caaccagaca taataaaaat actaattaat gatatagcag ctatcttttt catctcacct
    58381 tcccccatta aataccaacg acactctcta gtgtttaaat ataataatgg catgattatt
    58441 ataattgaat aggattataa taaatgttct gtacaacatt tcctacataa gtaggaatta
    58501 cggacattga ggcccttcag ggtaactcca tgggggcttt aatatatttg gaatagtata
    58561 tttaagatgc cactgtttgg ttgaatatta ggcatatgct cttttttgaa atttatcggt
    58621 ggcagccgtt agtattcgct gtccccattg caagctcctg gtggtaacca ctgaatcctc
    58681 catacttgaa ctgacttttt atcctccgac tttcatcctg ttctgactcc accttttgtt
    58741 ttctgctcta cactatctac agaccaatca taaaggcaca tacgatcatg gcagaatttc
    58801 ccgccagctt actgattctt aatggcaaaa gtactgacaa tctacccttg cgcgaagcaa
    58861 ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg ggtcacctgg gagaaaggcg
    58921 atgccgcacg atatgtagag gaggcccgga agttgggcgt cgcaacggtg attgccggtg
    58981 gtggcgatgg caccattaat gaagtttcta cggcgttgat tcagtgtgag ggggatgaca
    59041 tacccgcgct gggaattttg ccattaggaa ccgccaatga ttttgccacc agtgtaggga
    59101 ttcctgaggc actggataag gcgctgaaac tggcaattgc cggtaacgcc attgcgatag
    59161 atatggcgca ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc ggatttggga
    59221 cgcgtattac cacagaaacg ccggaaaaat taaaagccgc gctgggtggc gtctcttaca
    59281 tcattcatgg cttaatgcgc atggatactc tgcaaccgga ccgttgtgaa atccgcggtg
    59341 aaaactttca ctggcaaggt gacgccctgg tcattggtat tggtaacggg cgtcaggccg
    59401 gtggcggtca gcaattgtgt ccgaacgcgt taattaacga tggcttgctg caactgcgca
    59461 tttttaccgg cgatgaaatt cttccggctc tcgtatcaac cttaaaatct gacgaagata
    59521 acccgaatat tatcgaaggc gcttcgtcgt ggtttgatat acaagcccca cacgaaatca
    59581 cttttaatct tgatggcgaa ccgttgagcg gacaaaactt tcatattgaa atacttccgg
    59641 cggcgttgcg ttgtcgatta ccaccagatt gtccattatt gcgttaatca aactattctc
    59701 tcttgaacca gggagaatcc gaaatgccgg atgcggtgta aataccttat ccggcataca
    59761 gaacaatact acaccgtaat taattttacc cgtgtcttct gcaatgccag tttatccacc
    59821 tctgatacag catcatcggt aataataatg tcaatttttt cgattggcag tacctgatta
    59881 aaaccgcgac ggttaaattt cgatgaatcg agaacggcaa tcactttatt agcggcggtg
    59941 accatcgcac cgctaatcga ataaccttca ttaaaggtcg taataccatt aaccgcatca
    60001 ataccatcag caccgacaaa cattaaatcg gcattaatat cctgcaggga acgttcagca
    60061 atagatccat gcatagatcg cgttttatgg cggaccgtgc caccgcagac gaccagagta
    60121 atgtctttat tttcggaaag ggcaaacgca gccgggagac tgttggtaat caccgtgata
    60181 tttttggcgg tcattaatcc ttcggcgata agcattgtgg tacttccgct gtcgagaata
    60241 acggtcatcc cttcatggat cattttgact gccgcctgcg ctattctgtt tttcggcgcg
    60301 ctggcgagct gaaatcgctc tttaaacccg acttcctggg tctcggtttc actattaccg
    60361 gacattattt tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg ttcgagaaag
    60421 cgcaaatcgg cacggattgt cgcttccgag gcagcaaata ctcccgccag atcctgaaca
    60481 agcacggttc cctgttcatt cactaattgg atgatcttat tccttcgctc gaatgaattc
    60541 atcgtcattt acccatattc aattgtggct agtgtaaacg aagggggacc attggtgaat
    60601 gatctgtttt gcacaatacc gggccagcat cacgctggcc cgcggtttca gggaatgagc
    60661 aacactttgc ccggcatagc attacgagcg atgtcacgca ccgcctgggc gaagctttca
    60721 aagcttccac ggtgagcgat taatggctcc aggcttaact tacgttctgt cagcaaccgg
    60781 ctcgccgttt cccactcctg ccccggccaa gggctggagt agttcatcca actgccgata
    60841 accgtcagct ctttacgcaa tattttgcca aacgttgccg atgttaaatg cagatcctga
    60901 tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg caatctctac cgccagttcg
    60961 acagtttgcg gtacgccagc cgtctcgagg ataagctgat taaagcgcag ttagcgtaaa
    61021 acgctctgca tttgcggcgc gctcatttcg ctactgttaa atgtttgcat cgcaccgaaa
    61081 gattttgcca gtgccagttt ttctgaacta atgtcgatcg ccgtcacact ctttgctccc
    61141 agcgcgacag cgcactgaat ggccagcagg ccaatggttc cggcaccaat aataataacg
    61201 tttttattct cacaaccttg cgctaaatga aaagcatgca ggccaacggt aatcggttca
    61261 ataaaagccc catcctcaat aggcatatca gtaggtagag caaagacatt tttccgctta
    61321 acgacaatat attcggcaaa tccaccatca cgccgtgagc caataaaatc atattttgcg
    61381 cactgggaat aaaacccttt caaacactct ggacaagtaa aacagggtaa taacggcaca
    61441 caggcaaccg catcgccagg atgtaaatca tcaacaccgg atcccaccgc atcaatatag
    61501 ccgctaaatt catggcctaa cgttattgga taataatgtg caccattttt aaatatcctg
    61561 ggtaaatcgg aaccacataa gcccgagctg gcaattttta cccgcacctc atcctgatgt
    61621 ttaatttcag gaatgacgct ttctgcaacg cgcacgatac catcagtatc attcaccact
    61681 gatttcataa aaactcctga ttgttaaggg ggataacctc cccctcaggt aaaaattatt
    61741 ctgcgagaac gactttctct tgtttaataa agccacgcgc tctacgccag gtcatgaaaa
    61801 taccggtaag ataaattgcg ccgataataa tgaatccggg aatattttgc ggggagaaaa
    61861 cctgaatcag taaccaggta atgggagaac cgccctgatc cattgaagcc accatacccc
    61921 cggcttttaa cgccccagca ttagccgcca gttgggtgtg caaaccaata gtttgcgtcg
    61981 cgatccacag agtgatgctc atgatgatga cacccgagat taaggtgcgg aacagatttc
    62041 cacgatgcac ggcgactgcc atcgccacaa agaagccgat ggtggcaaga tcgccaaacg
    62101 gcagcacctg attacccgga acacaaacag caattaaaat ggtgagcggg ataaaaatca
    62161 ggcttgccga taccaccgcc gtatgcccca gcagtaatgc gggatccagg ccaatcagga
    62221 actcctgacc gccgaacttc gcctgtaaac gactacgggc ctgcttagcg atgggcgtta
    62281 aaccatccat gatgggttta atcacccgtg gcattagcag catcactgcc gccgttttta
    62341 ccgccagctg caatacacct ttgacatcgt aacccgcgag gatgccgata atcagcccca
    62401 tcacaaaacc cacggtgaca ggctcgccaa atggaccaaa tttgcgctga atatcgtcgg
    62461 cgctaaattt aattcggtta acgcctggga ttttctcgat gatagcatcg accagcaccg
    62521 caatcggccc catatacgcc gacgtaccgt gcggaatagc aataccttcc agctcaaaga
    62581 aatttcgggt atcgcgggca aaccagtcgc cgagcttata aacaaacgcc gcgtgaatta
    62641 ccacacctgc catccctatc atccatgaac cggttgccag gtgcagcaac gcgccggtga
    62701 aggtcatatg ccagatattc cagatatcaa catttaccac ccgcgtcata cgggtcagta
    62761 acatcgccac gttaaccaat atcgcaatcg gaatcgccac cagcgcaatt tgcgacgccc
    62821 aggtcattgg tgaagagccc ggccagccga catcgaccac atgcagattc aggtcgaaat
    62881 tttccgccat cgctttcgcc gccggaccaa tggaatccag cattaagcca atcacaaggc
    62941 caatgccaac aaagccaatc ccgatatgca gacccgcttt aaagcaatcg cctgccttca
    63001 tgcctaatat tttagaaaaa ataatgatga caatcggaag catcaccgta gggccgaggt
    63061 cgagaatata acgcatgact tctgaaaaca taggtcaccc ctgtaagata gtcagaattt
    63121 tattttgtaa tgcttcgata ccgacaccag aaataaaagg catgccgtga actaacggaa
    63181 tatcgccaaa actacgatcc actttggcag tggtgcatat caaatgcaca ccatccatat
    63241 aggtttctat ttcattaacc cgacactgga ttaattcaac aggaatatta tgattctgac
    63301 acaactcttt aatttcttcc gccgccatcg tagaggtcgc aaccgcgcct ccgcaagcga
    63361 caataatctt gcgtttcata aatagtcctt tttattgtta cagagggatt tattcgtctg
    63421 aatctaaaac atactttgtg aagtattcct ttaactgagt ttcaggaaga gtaattagtg
    63481 tctcgacgat atcgggctgt tgtaacttgc caaataaaca gcgtaaaagt ttcaattgct
    63541 gctgcggatt ttccacaatc aacgcaataa ccaacgacac ctccacgtcg ttatcatcat
    63601 ccgcttgctg aaaatgaact ttatttgttg gccttaacag ataaatggct gacgacttag
    63661 catgaatcgc ctcacaatgc ggtatagcaa tggcgtgctg ctcaagcatt atcccggtag
    63721 ggaattctgc ttctctggca attaatgcct gtggccaggt atcataaacc acacctttcg
    63781 cgagcatctc attaccgata tgggttaaaa cttcgctacg atcgacaaaa gaaattccgc
    63841 tacgaacaaa caggttagtc atataccgtc cttattccgc acagccgtag cgataggcgc
    63901 gcaaaacatc ataaatttta tccataatca gctgatgcgg tattgctgat aattccccgg
    63961 actgaatgcg ttcaaattgt ttgggaagat actgactaat catgcccagt gggatgtcca
    64021 cgccttcaag attcaccatc atcgtttcga cgctattttt aatccgacta tgcggccagt
    64081 aataacgaat acgatccgac aggctgtaac gaatatccag taatgaatcg ttaaaacccg
    64141 tacgataata ttttttccag tattgcggtt cgtcgagcat cacttcttca attaccgcca
    64201 ggcaaccgct gcgattttca ggggcgataa gttcctgctc aatttgtgcc agtgcaaata
    64261 tcgcctcgcg taaagcaaag gttaatgcgg gaccgacttt caatattgca aagtgatcgc
    64321 ggactaattc ccaataagcc gtccgggtct ggtaatcggt agaatgtgct tcataaacca
    64381 ttcgggtgct ttctatccat tgcgccagcg gctgcgcttc ctgcggctga taatggataa
    64441 tattgctgtg atcaaattcc acgcccggct gcaccacgat ggcaatcacg cgtgttaacg
    64501 cctctgtcag cccacgggca ataaaggcct tttgatgcgt acgtaaagta ttggcggcat
    64561 cttcaacatg ggtgatgtgt actgactgaa tggcgctggc ctcaccgccc ggaaccggaa
    64621 cttcggtgcc aatgacatag ctcagttgct cacgctggca atctgtcgcc acactttccg
    64681 cagcaaagca aagcacagca gctcgttccg caaccgtttc tggtgctaac ggtatgggat
    64741 cccccgcgca ggacattgac gcatcaagat gaattttact gaaaccggca cgaacatatt
    64801 cctttaccag ctcgacggat ttttccatcg ccgcatccgc attttcttgc tgccagcagt
    64861 ttggccccag atgatcgccg ccgagaataa tgcgttcgcg tgcaaaccca actttatcgg
    64921 caatcgtaaa aacaaattcg cgaaagtctg ccggtgtcat tccggtataa ccgccaaatt
    64981 gattgacctg gtttgacgtt gcttcaatca gcactttgcg cgtgctgttg cgatcaaatg
    65041 ccagcgccgc ttcgataacc aacggatggg cagaacagac tgaacatatg ccgatatgtt
    65101 caccagcttt atgccgggca attaacgttt tcatgttttt tccttattaa atggcaggtg
    65161 cgttatgccc tgccctcgca gccacaatcg gcaatcactt tgctcaccac atcgcgcatt
    65221 gcggatttag ccgactgcaa ataatcccgg ggatcggtcg cttcagggtg ctcggtcagg
    65281 taatttttta acgcctgcga gaaggcattt ttcagctccg ttgcaacgtt gattttgcat
    65341 atccccagtt tgatggtttg ctgaatatcc ttagtcgata accctgacgc gccatgcagc
    65401 accagcggta agttcaccca ctggcgaatg ttctccagtc gagaaaaatc aagcgccggt
    65461 gcgctggcat acatcccatg agccgtgccg atggcgaccg ccagggaatc aattccggtt
    65521 gcctcggcaa attcacgcgc ctgagcgggg ttggtataaa aagcatcggc ttcattgact
    65581 tgcacatcat cttcctggcc gccaagttgc cccagctccg cttcgacgct gacatcaaag
    65641 cgatggcaaa aatccaccac ctctttgacc cgtgaaatat tttgcgcaaa aggcaaatgc
    65701 gaggcgtcaa tcatgactga gcgcacgcca gaacgaacct tctgagcgat atcgtcaaat
    65761 ttcgtgtgat ggtcgagatg aattgccagt ggatggtgat attgcttcgc catcgcgctg
    65821 accagcgcca acagattttc tgtaccagca tgagtaaatg tgccaggcgt tccggcgatg
    65881 atgaccggcg catgcaggtt ggcagcggtt tccaccacca cttgcatcgt ttcgagattg
    65941 tgaatattga atgccggaac cgcataaccg ccgcgctgtg cgttgttcag catctgcttt
    66001 gtcgatacca cgtacatttt catatcctgt cgtttgtttt cgatttcaaa atataatgaa
    66061 atgatttgtt tttaaatgtc gagataacga tcacaaaagc gacaatgtga aaattgttcg
    66121 aggagtaaaa ggc
//
LOCUS       DAZGOB010000009        62875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGOB010000009 DAZGOB010000000
VERSION     DAZGOB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.009"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(65..1309)
                     /gene="mtr"
                     /locus_tag="ACHLIF_000600"
     CDS             complement(65..1309)
                     /gene="mtr"
                     /locus_tag="ACHLIF_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO9604668.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="ACHLIF_000601"
     CDS             complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="ACHLIF_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO9604669.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="ACHLIF_000602"
     CDS             complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="ACHLIF_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO9604670.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="ACHLIF_000603"
     CDS             complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="ACHLIF_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO9604671.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="ACHLIF_000604"
     CDS             complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="ACHLIF_000604"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO9604672.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="ACHLIF_000605"
     CDS             complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="ACHLIF_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO9604673.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7325..8269)
                     /gene="truB"
                     /locus_tag="ACHLIF_000606"
     CDS             complement(7325..8269)
                     /gene="truB"
                     /locus_tag="ACHLIF_000606"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO9604674.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="ACHLIF_000607"
     CDS             complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="ACHLIF_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO9604675.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8834..11506)
                     /gene="infB"
                     /locus_tag="ACHLIF_000608"
     CDS             complement(8834..11506)
                     /gene="infB"
                     /locus_tag="ACHLIF_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO9604676.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="ACHLIF_000609"
     CDS             complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="ACHLIF_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO9604677.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="ACHLIF_000610"
     CDS             complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="ACHLIF_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO9604678.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13705..13781)
                     /locus_tag="ACHLIF_000611"
     tRNA            complement(13705..13781)
                     /locus_tag="ACHLIF_000611"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13745..13747),aa:Met,seq:cat)
     gene            14129..15472
                     /gene="argG"
                     /locus_tag="ACHLIF_000612"
     CDS             14129..15472
                     /gene="argG"
                     /locus_tag="ACHLIF_000612"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO9604679.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15480..17105)
                     /gene="yhbX"
                     /locus_tag="ACHLIF_000613"
     CDS             complement(15480..17105)
                     /gene="yhbX"
                     /locus_tag="ACHLIF_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9604680.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(17564..17650)
                     /locus_tag="ACHLIF_000614"
     tRNA            complement(17564..17650)
                     /locus_tag="ACHLIF_000614"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17614..17616),aa:Leu,seq:gag)
     gene            complement(17665..17997)
                     /gene="secG"
                     /locus_tag="ACHLIF_000615"
     CDS             complement(17665..17997)
                     /gene="secG"
                     /locus_tag="ACHLIF_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO9604681.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="ACHLIF_000616"
     CDS             complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="ACHLIF_000616"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO9604682.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19555..20403)
                     /gene="folP"
                     /locus_tag="ACHLIF_000617"
     CDS             complement(19555..20403)
                     /gene="folP"
                     /locus_tag="ACHLIF_000617"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO9604683.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20493..22427)
                     /gene="ftsH"
                     /locus_tag="ACHLIF_000618"
     CDS             complement(20493..22427)
                     /gene="ftsH"
                     /locus_tag="ACHLIF_000618"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO9604684.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22527..23156)
                     /gene="rlmE"
                     /locus_tag="ACHLIF_000619"
     CDS             complement(22527..23156)
                     /gene="rlmE"
                     /locus_tag="ACHLIF_000619"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO9604685.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23282..23575
                     /gene="yhbY"
                     /locus_tag="ACHLIF_000620"
     CDS             23282..23575
                     /gene="yhbY"
                     /locus_tag="ACHLIF_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO9604686.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23731..24207)
                     /gene="greA"
                     /locus_tag="ACHLIF_000621"
     CDS             complement(23731..24207)
                     /gene="greA"
                     /locus_tag="ACHLIF_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO9604687.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24455..25888
                     /gene="dacB"
                     /locus_tag="ACHLIF_000622"
     CDS             24455..25888
                     /gene="dacB"
                     /locus_tag="ACHLIF_000622"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO9604688.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25928..27100)
                     /gene="cgtA"
                     /locus_tag="ACHLIF_000623"
     CDS             complement(25928..27100)
                     /gene="cgtA"
                     /locus_tag="ACHLIF_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO9604689.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27116..28081)
                     /gene="yhbE"
                     /locus_tag="ACHLIF_000624"
     CDS             complement(27116..28081)
                     /gene="yhbE"
                     /locus_tag="ACHLIF_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9604690.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28208..28465)
                     /gene="rpmA"
                     /locus_tag="ACHLIF_000625"
     CDS             complement(28208..28465)
                     /gene="rpmA"
                     /locus_tag="ACHLIF_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO9604691.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28486..28797)
                     /gene="rplU"
                     /locus_tag="ACHLIF_000626"
     CDS             complement(28486..28797)
                     /gene="rplU"
                     /locus_tag="ACHLIF_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO9604692.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29056..30027
                     /gene="ispB"
                     /locus_tag="ACHLIF_000627"
     CDS             29056..30027
                     /gene="ispB"
                     /locus_tag="ACHLIF_000627"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO9604693.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30254..30532
                     /gene="sfsB"
                     /locus_tag="ACHLIF_000628"
     CDS             30254..30532
                     /gene="sfsB"
                     /locus_tag="ACHLIF_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO9604694.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30580..31839)
                     /gene="murA"
                     /locus_tag="ACHLIF_000629"
     CDS             complement(30580..31839)
                     /gene="murA"
                     /locus_tag="ACHLIF_000629"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO9604695.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31894..32148)
                     /gene="ibaG"
                     /locus_tag="ACHLIF_000630"
     CDS             complement(31894..32148)
                     /gene="ibaG"
                     /locus_tag="ACHLIF_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO9604696.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32308..32601)
                     /gene="mlaB"
                     /locus_tag="ACHLIF_000631"
     CDS             complement(32308..32601)
                     /gene="mlaB"
                     /locus_tag="ACHLIF_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO9604697.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32601..33236)
                     /gene="mlaC"
                     /locus_tag="ACHLIF_000632"
     CDS             complement(32601..33236)
                     /gene="mlaC"
                     /locus_tag="ACHLIF_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO9604698.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33255..33806)
                     /gene="mlaD"
                     /locus_tag="ACHLIF_000633"
     CDS             complement(33255..33806)
                     /gene="mlaD"
                     /locus_tag="ACHLIF_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO9604699.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33811..34593)
                     /gene="mlaE"
                     /locus_tag="ACHLIF_000634"
     CDS             complement(33811..34593)
                     /gene="mlaE"
                     /locus_tag="ACHLIF_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO9604700.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34601..35410)
                     /gene="mlaF"
                     /locus_tag="ACHLIF_000635"
     CDS             complement(34601..35410)
                     /gene="mlaF"
                     /locus_tag="ACHLIF_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO9604701.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35620..36597
                     /gene="yrbG"
                     /locus_tag="ACHLIF_000636"
     CDS             35620..36597
                     /gene="yrbG"
                     /locus_tag="ACHLIF_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO9604702.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36611..37597
                     /gene="kdsD"
                     /locus_tag="ACHLIF_000637"
     CDS             36611..37597
                     /gene="kdsD"
                     /locus_tag="ACHLIF_000637"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO9604703.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37618..38184
                     /gene="kdsC"
                     /locus_tag="ACHLIF_000638"
     CDS             37618..38184
                     /gene="kdsC"
                     /locus_tag="ACHLIF_000638"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO9604704.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCVLTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            38181..38756
                     /gene="lptC"
                     /locus_tag="ACHLIF_000639"
     CDS             38181..38756
                     /gene="lptC"
                     /locus_tag="ACHLIF_000639"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO9604705.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38725..39282
                     /gene="lptA"
                     /locus_tag="ACHLIF_000640"
     CDS             38725..39282
                     /gene="lptA"
                     /locus_tag="ACHLIF_000640"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO9604706.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39289..40014
                     /gene="lptB"
                     /locus_tag="ACHLIF_000641"
     CDS             39289..40014
                     /gene="lptB"
                     /locus_tag="ACHLIF_000641"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO9604707.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40062..41495
                     /gene="rpoN"
                     /locus_tag="ACHLIF_000642"
     CDS             40062..41495
                     /gene="rpoN"
                     /locus_tag="ACHLIF_000642"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO9604708.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41518..41805
                     /gene="hpf"
                     /locus_tag="ACHLIF_000643"
     CDS             41518..41805
                     /gene="hpf"
                     /locus_tag="ACHLIF_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO9604709.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41923..42414
                     /gene="ptsN"
                     /locus_tag="ACHLIF_000644"
     CDS             41923..42414
                     /gene="ptsN"
                     /locus_tag="ACHLIF_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO9604710.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42460..43314
                     /gene="rapZ"
                     /locus_tag="ACHLIF_000645"
     CDS             42460..43314
                     /gene="rapZ"
                     /locus_tag="ACHLIF_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO9604711.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43311..43583
                     /gene="npr"
                     /locus_tag="ACHLIF_000646"
     CDS             43311..43583
                     /gene="npr"
                     /locus_tag="ACHLIF_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO9604712.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43797..44429
                     /gene="yrbL"
                     /locus_tag="ACHLIF_000647"
     CDS             43797..44429
                     /gene="yrbL"
                     /locus_tag="ACHLIF_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO9604713.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(44426..45154)
                     /gene="mtgA"
                     /locus_tag="ACHLIF_000648"
     CDS             complement(44426..45154)
                     /gene="mtgA"
                     /locus_tag="ACHLIF_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO9604714.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45151..45804)
                     /gene="elbB"
                     /locus_tag="ACHLIF_000649"
     CDS             complement(45151..45804)
                     /gene="elbB"
                     /locus_tag="ACHLIF_000649"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO9604715.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46034..48370)
                     /gene="arcB"
                     /locus_tag="ACHLIF_000650"
     CDS             complement(46034..48370)
                     /gene="arcB"
                     /locus_tag="ACHLIF_000650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO9604716.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48466..49395)
                     /gene="yhcC"
                     /locus_tag="ACHLIF_000651"
     CDS             complement(48466..49395)
                     /gene="yhcC"
                     /locus_tag="ACHLIF_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO9604717.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCSNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            50070..54530
                     /gene="gltB"
                     /locus_tag="ACHLIF_000652"
     CDS             50070..54530
                     /gene="gltB"
                     /locus_tag="ACHLIF_000652"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO9604718.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            54543..55961
                     /gene="gltD"
                     /locus_tag="ACHLIF_000653"
     CDS             54543..55961
                     /gene="gltD"
                     /locus_tag="ACHLIF_000653"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO9604719.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANDGRIFEAAELSHQTNPLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            56145..57192
                     /gene="yhcG"
                     /locus_tag="ACHLIF_000654"
                     /pseudo
     CDS             56145..57192
                     /gene="yhcG"
                     /locus_tag="ACHLIF_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(57252..57716)
                     /gene="nanQ"
                     /locus_tag="ACHLIF_000655"
     CDS             complement(57252..57716)
                     /gene="nanQ"
                     /locus_tag="ACHLIF_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO9604720.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(57713..58588)
                     /gene="nanK"
                     /locus_tag="ACHLIF_000656"
     CDS             complement(57713..58588)
                     /gene="nanK"
                     /locus_tag="ACHLIF_000656"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO9604721.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(58585..59274)
                     /gene="nanE"
                     /locus_tag="ACHLIF_000657"
     CDS             complement(58585..59274)
                     /gene="nanE"
                     /locus_tag="ACHLIF_000657"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO9604722.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(59322..60812)
                     /gene="nanT"
                     /locus_tag="ACHLIF_000658"
     CDS             complement(59322..60812)
                     /gene="nanT"
                     /locus_tag="ACHLIF_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO9604723.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(60922..61815)
                     /gene="nanA"
                     /locus_tag="ACHLIF_000659"
     CDS             complement(60922..61815)
                     /gene="nanA"
                     /locus_tag="ACHLIF_000659"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO9604724.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(61937..62728)
                     /gene="nanR"
                     /locus_tag="ACHLIF_000660"
     CDS             complement(61937..62728)
                     /gene="nanR"
                     /locus_tag="ACHLIF_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO9604725.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        15387 a        17089 c        15522 g        14877 t
ORIGIN      
        1 cctgataagc gaagcgcatc aggcattgtg taggcagcag aaatgtcgga taaggcaccg
       61 ctgattactg atacaccggc agtaaattaa agctcgataa aatatggacc agtgcgttgc
      121 caacgccaaa caccagaatc agcgtaatca tcggcttgcc gccccagacg cggaatttcg
      181 ggctgccaaa gcgtttacgc gatgcgcggg ctaacagcgc cggaacaatt gccgcccaga
      241 tggtagctgc taaaccagca taaccaatgg cgtacaggaa tccgttcggg aacagtagac
      301 cacccacaac aggcggggca aaggtcagca atgccgtttt caagcgcccc agggctgagt
      361 catcgaaacc aaacagatct gccagatagt caaacaaacc cagcgttacg ccaaggaacg
      421 aactcgctac cgcaaagttt gagaacacga ccagcagaag gtccaggcta cggctgttca
      481 gcacgccgct taatgcctgt accagcacat caatattacc gcccttctct gcaataccga
      541 taaactccgg acgcgggatg ttacccatcg tcgccagcaa ccagatggta tacagcgcca
      601 gtgccatcag cgtaccgtac accaggcatt tcacgatggt tttcggatct ttgccgtaat
      661 acttcatcag gcttggcacg ttaccgtgat aaccaaacga cgccaggcag aacggcaatg
      721 tcatcagcag atacggcgca taagatgcat tgctttctgc aacgttgaac aatgtcgcag
      781 gctgcacatg ccccaacagg ctgccaaagg tgaggaagaa ggtaatgact ttcgccccca
      841 gcacaatcgc cgtcatgcgg ctgacggcct tagtactcaa ccacaccaca aacgctacca
      901 gcagtgcaaa accaaaaccc gccgcccgtg ccgggacgtt tagcgacatc tctgcgaagg
      961 tgtgatgcag aattgaacca ctggcagaaa tataggcata ggtcaggata tagagcacaa
     1021 aggcaatgga aataccgttg accacgttcc agcctttgcc cagcaaatct ttggtgatgg
     1081 tgtcaaaact cgaaccgatt cggtaattca ggttagcttc cagaatcatc aagccggaat
     1141 gcagcataca gaaccaggta aagatcagcg ccgccattga ccagaaaaac cacgccccgg
     1201 acatgaccac tggcagagaa aacatccctg cgccaataat ggtgccgccg ataatcacca
     1261 cgccgccaag cagcgacggt gacgtttggg tggtggttag tgttgccatg agggcttctc
     1321 tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca ctgtaccagt acacgagtac
     1381 aaaagacaga aaaaaagccc cgatggtaaa aatcggggct gtatatatta ttttacagat
     1441 tgtgttcgct gttcagcgat gattacgcat caccaccgaa acgacgacga ccggtagagt
     1501 catcacgacg cggagcgcgg ccttcacgac gttcgccgct aaaacgacga ccatcaccac
     1561 ggccaccttc acggcgttca ccgctgaagt tacgaccgcc ttcacgacgt tcgccaccga
     1621 aaccacgacc accgccacga cgctcaccgc cagtatgcgg ctgcgcatcg cccagcaact
     1681 gcatattcat cggcttgttc agaatgcgag tacgcgtaaa gtgttgcagt acttcacccg
     1741 gcataccttt cggcagttcg atagtggagt gagaagcaaa cagcttaatg ttaccgatgt
     1801 aacggctgct gatgtcgcct tcgttagcaa tcgcgccaac gatatgacga acttcaacac
     1861 catcatcgcg gcccacttca atgcggtaaa gctgcatgtc accaacatca cgacgttcac
     1921 gacgcggacg atcttcacgg tcgccacgtg ggccacggtc gttacgatcg cgcggaccac
     1981 ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg cgcatctggc ggaacaatca
     2041 gagtacgttc accctgtgcc attttcagca gtgccgcagc cagagtttcg agatcctgct
     2101 cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc gcggtattga tccagatcgc
     2161 tgctttccag ctgctgctgt actttagcgg cgaatttttc cagacggcgt ttgcccagca
     2221 gttctgcgtt cggcagttct acttccggaa tagtcagctt catagtacgt tcaatgttgc
     2281 gcagcagacg acgctcgcgg ttctcaacga acagcagcgc gcggccagca cgacccgcac
     2341 gaccggtacg accgatacgg tgaacgtaag actcagaatc catcgggata tcgtagttaa
     2401 ctaccaggct gatacgctca acgtccaggc cacgggctgc aacgtcggtc gcaatcagga
     2461 tgtccagacg accatctttc aggcgttcca gtgtctgttc acgcagcgcc tggttcatgt
     2521 caccgttcag cgcggcgctg ttgtagccgt tacgctcaag agcttcagcc acttccagag
     2581 tcgcgttttt ggtacgaacg aagataatcg ccgcatcaaa atcttccgct tccaggaaac
     2641 gtaccagtgc ttcgtttttg cgcatacccc agacagtcca gtagctctgg ctgatgtcag
     2701 gacgggtagt cacgctggac tgaatgcgca cttcctgcgg ctctttcata aagcggcggg
     2761 taatgcgacg aatcgcttcc ggcatggttg cagagaacag agcggtctga tgaccttccg
     2821 ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc catgcgcagc atttcgtcag
     2881 cttcatccag aaccagaccg ctcagtttag agaggtccag agtgccacgt ttcaggtggt
     2941 ccagcagacg gcccggagta ccgacaacga tctgcggccc ctgacgcagg gcgcgtaatt
     3001 gcacgtcata acgctggccg ccgtatagag caaccacatt tacgccgcgc atgtgtttag
     3061 agaaatccgt cattgcttca gcaacctgta ccgccagttc gcgggtcggt gccagcacca
     3121 gaatctgtgg tgctttcagc tcaggatcaa gattctgcaa cagaggtaaa gagaatgctg
     3181 cagtttttcc gctccccgtc tgggccatac ccagaacgtc gcggccattc agcagatgtg
     3241 gaatacactc tgcctgaatt ggagatggtt tttcgtaacc cagatcgtta agggcttcaa
     3301 ggataggagc cttcaggccc agatctgcaa aagtggtttc gaattcagcc atgtagtacg
     3361 tgtgcctcaa aattaatggc ggccagtcta cataactcat catgaaattg atcagcaatt
     3421 ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta acgaacaaca acgccctcac
     3481 ccgttaaggt gatggcaatc aaaaaagatt acgggctgat gtgtacgtca gctattgctg
     3541 gtccgattct gccaggtcat cttggtcctg gcccaggagc gataattcca acaatgcgta
     3601 tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc agtttgaaca gtgccgtggc
     3661 gctgtccaaa tcccccagac ttaggtagta cttacctaaa tagaagttgg tttcactgag
     3721 atgctcagcg agcgaggtgt tatccgttgc gtccgccttg agcctttcca ttaacgtttg
     3781 ttcgctaatg ttgcccaggt agaactcgac aatgttccat ccccactgtt ccttatccga
     3841 tttttcgaag tgctgtttca acacttcttt agcctgcttc tcatcgagct tctgctcggc
     3901 gagataaagc cacagactac ggaaaggatc attgggatcg tcttgataaa acgccagcag
     3961 atcatcttgc gctaacttgt cacgaccgcc gtaatataat gcgatcccgc gattcaagtg
     4021 cgcgtagttg taagttggat caagctcaag tacagaatca aacgcttcat aggcagcatc
     4081 aaaattgcct gcctgcgtta aatatatgcc taagtaattg aatacttcag gcatatccgg
     4141 tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat gccctcagac cgagactatc
     4201 atacaacact ccgcgctcat ataaaagctg tgcgcgttcg tcatcggtta aagcccgact
     4261 ggcaaggatt tgttccatac gtgccagaat cacttcctgc tgtaaagtcg gttgcaatgg
     4321 taccgcgagg acttcacttt tacgccagga agtattactg catcctgcaa gcgtaagtgc
     4381 tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc cactcccgaa gaccacggtt
     4441 gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg cccggttaaa agccccccgc
     4501 cgcagcggag ggcaaatggc aaccttactc gccctgttca gcagccggag cttccggtgc
     4561 tgcagcaggt tgagactgct cagtcgcttc tttaatgctc agacggatac ggccctggcg
     4621 atcaacttcc agaactttca ccggtacttc ctgacccatc tgcaggtaat cggtcacttt
     4681 ctcaacgcgt ttgtcagcga tttgagagat gtggaccaga ccttctttac cgccgccgat
     4741 ggcaacaaat gcgccaaagt caacgatacg ggtcacttta ccagtgtaga cgcggcccac
     4801 ttcgatttct gcagtgatct cttcgatacg acgaatagca tgtttcgctt tctcgccgtc
     4861 ggtcgctgcg atcttcacag taccgtcatc ttcgatttcg atggtagtgc cagtttcttc
     4921 ggtcagggca cggataacag agccgccttt accgataacg tctttgatct tgtccgggtt
     4981 gatcttgatg gtatggatac gcggtgcgaa ctcagagata tcgccacgcg gcgcgttgat
     5041 cgcctgttcc attacgccca ggatatgcag acgcgcacct ttagcctggt tcagcgcaac
     5101 ctgcatgatc tctttggtga taccttcaat tttgatatcc atctgcaatg cagagatacc
     5161 ctcgcgggaa cccgcaactt tgaagtccat atcgcccagg tgatcttcgt cgcccaaaat
     5221 gtcagacagt acaacgtagt tgtcgccttc tttcaccaga cccattgcga tacccgcaac
     5281 ggcagctttg atcggcacac ctgcgtccat cagcgccaga gacgcgccgc acacggaagc
     5341 catagaagag gaaccgttgg attcagtgat ttcagacaca acacgtacgg tgtacgggaa
     5401 tttgtccata tccggcatga ctgccagcac gccgcgcttc gccagacgac cgtgaccaat
     5461 ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct acggagtacg gagggaagtt
     5521 gtagtggaac aggaaggtat cggtacgttc gcccatcagt tcatcaagaa cctgcgcgtc
     5581 acgagcagta cccagcgttg cggtaaccag cgcctgcgtt tcaccacggg tgaacagcgc
     5641 agaaccgtga gtacgcggca gcacgccagt acgcacatcc agaccacgga tcatatcttt
     5701 ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg ctacgaacaa cgtttttctc
     5761 gatagcgtgc agaatttcac ccagttcgtt ttcgtccagg gtttcgtctt cagcaagcag
     5821 cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca taacgctctt gtttgtcggt
     5881 gatgcggtaa gcatcgctca ggcgagcttc agccagtgca gcaacgcgcg cgttcagcgc
     5941 ttcgtttacc ggctccggct gccagtccca acgtggttta ccggcttctt tcaccagttc
     6001 attgatgttc tgaataacaa cctgctgttg ttcatgaccg aacactactg cgcccagcat
     6061 ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc agaacagcgg cttcagtacc
     6121 ggcaacaacc agatccagtt tgctctcttt cagctcgtcc tgagtcgggt tcagtacgta
     6181 ctggtcattg atgtaaccta cgcgagcagc accaatcgga ccattgaacg gaataccaga
     6241 cagagacagt gctgcggaag caccaatcat cgcgacgata tccgggttaa cttgcgggtt
     6301 aacagaaacc acggtggcga taacctgaac ttcgttgacg aagccttccg ggaacagcgg
     6361 gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg ccttcgcttg ggcggccttc
     6421 acgacggaag aagctacccg ggatacgacc agcagcgtag gtacgctcct gatagttaac
     6481 ggtcagtggg aagaagtcct gacctggttt ggcttttttc tggccaacaa cggtaacaaa
     6541 taccgcggtg tcatccatgc taaccataac agcggcagta gcctgacgag ccatcatgcc
     6601 ggtttccaga gtcacggtgt gttggccgta ctggaattta cgaacgatcg gattaagcaa
     6661 tgtaatatcc tttctctttc ttagacagta ccttacggca ctggtgttaa tacccgatct
     6721 tctgcgcatc ctcgcgacta atgacaaccc taacccagct ctatgtgggt aaagcctctc
     6781 attagccgcg cgaacctctg caacggaaga tcattcatag caacaataca ttagtttcca
     6841 gtgaattgct gccgtcagct tgaaaaaagg ggccactcag gccccctttt ctgaaactcg
     6901 caagaattag cgacgcagac ccaggcgctc gatgagctgg gtgtaacgtg ctacgtcttt
     6961 acgtttcagg tagtcgagca gtttacgacg ctgagaaacc atgcgcagca gaccacgacg
     7021 gctgtggtga tcttttttgt gctctgcaaa gtggccctgc aggtggttga tctgtgcagt
     7081 cagcagtgct acctgaactt cggtagaacc ggtgtcgttt gcgtcacgac caaactcaga
     7141 aacgatttta gctgttgctt cagtacttag agacatttta aaactccaaa gtatatagaa
     7201 tgaaaggacg ccgatctcta attcagcgat cccagtgtac gttacgcaaa gtgttaaaca
     7261 atttacgcga cgttaagcgg cagtattcta ctcgtagcga cctgttatcg caagacggtt
     7321 aacattacgc cgggtattca accaccaggc gacgaggcgc aacgcggcct tcatcgtcaa
     7381 tttcgcccat accgataaat ttgccgttct caccttctgt aacgcgaacc agtccttcca
     7441 gcggcgcacc ggatgtacga actgggttac catttttgaa gtaaacagaa gacgttaacg
     7501 gaagattcac caccgggtag tccgaagctg gactgtccat tggcatcagt aatggatcaa
     7561 gtaactccgc agccggaata tcctgctgtt cggcttgttc gaccagttca cgcagatgtt
     7621 ccagggtcac catccgttca accggatatt tacttaccgc cagacgacgc aggtaaataa
     7681 catgcgcgcc acaaccgagt ttttcaccca ggtcatcaat gatggtgcgg atataagtgc
     7741 cttttgagca gtgaatttcc agctccagct cattgccttc gtggcgaata aacagcaatt
     7801 cataaacggt aatcgggcgc gcttcacgcg gtacttcaat gccctgacgc gcatattcgt
     7861 acagtttttt accctgatat ttcagtgctg aatacatcga agggatctgt tcgatatcgc
     7921 cacggaaagt atccagcgcc gccgccagtt gctctgcgct aaaggttacc ggacgttctt
     7981 caacgatttg tccgtcggca tcagaagtat cggtacgctg tccaagacgc gcaatgaccc
     8041 gatagcgttt gtcggagtcc agcagatact gggaaaactt cgtcgcttcc ccgaggcaaa
     8101 ttggcaacat gccagttgcc agcgggtcca gcgcaccggt atgcccggca cggttggcgt
     8161 tataaatccg tttcactttt tgcagcgcat cattgctgga cataccctgc gatttatcca
     8221 gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg acgactcatt agtcctcctt
     8281 gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg accacgctgg tcaccaggtt
     8341 tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag aaggtcagtt ccggcacgat
     8401 acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa ccagaagctt cttgcaacgc
     8461 tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg aggaacgtca catatacttt
     8521 ggcatacgcc aggtcgcgag acatttcgac accggaaacg gtggtcatca tgcccaggcg
     8581 aggatcttta atttcacgct gcaggatgag agcgatctct ttttgcattt cctgcgctac
     8641 gcgctgcggg cgaccaaatt ctttcgccat aataaattct cctgacaaaa aaggggctgt
     8701 tagccccttt ttaaaattaa tttcaggtgg aagggctgtt cacgttggcc tgataagacg
     8761 cgccagcgtc acatcaggca atccatgccg gatgcggcat aaacgcctta tcccgcatgg
     8821 aaccctaaaa accttaagcg atggtacgtt ggatctcgat gatttcgaat acttcgatca
     8881 catcgccagt gcggacgtcg ttgtagttct taacgccgat accacattcc ataccgttac
     8941 ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct tcgtagataa
     9001 ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt aaccacacct tcggtaacca
     9061 tacagcctgc gatggcacca aatttcggcg atttgaacac gtcacgaact tccgccagac
     9121 cgataatctg ctgtttcagt tccggagaca gcataccgct catcgccgct ttcacttcgt
     9181 caatcaggtt atagatgacg gagtagtaac gcagatccag gctttccgct tcaatcactt
     9241 tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg gacgccgcag
     9301 ccagggtggc gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa
     9361 cttcgtcagt agacagtttc agcaaggagt cggagatcgc ttccacagaa ccctgtacgt
     9421 ctgccttcag gacgatgttc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt
     9481 tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat
     9541 acagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag
     9601 ccggtacgcc ggacaggccg aggatttcca ccggaatgga cggacccgct tccagcactt
     9661 cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca cacagaacga
     9721 tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt
     9781 tatcgaggaa ggattcgata accgcaccgc tcgccatacc tttacgtacc gctttcagct
     9841 ccagaacttc cgcctgcagc aggatagcgt ccagcagttc atcgatgccg gtacccgctt
     9901 tcgcagatac gtgtacgaac tggctttcac cgccccactc ttccggcagg atgccatact
     9961 gggagagttc gttcttaaca cgatccggat cggcttctgg tttatcaatc ttgttcactg
    10021 caaccaccac cggtacctgc gccgctttcg cgtgctggat tgcttcgatg gtctgcggca
    10081 tcacgccgtc gtcggcagca acaaccagga ctacgatgtc cgttgcctgc gcaccacgtg
    10141 cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt
    10201 cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcaa
    10261 ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga
    10321 tggtcacaac cggcgcgcgc ggttcagccg cagcacccgt gtcacggtcg ctcattaccg
    10381 cctcttccag ctcgttttca cgacgcagga taactttgtg gcccatctct tcagcaacca
    10441 gctgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca
    10501 tcgctttgat gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga
    10561 tggtttcgcc gatcacaacg tcacggttaa cggcctgagc tggcttctgg aagccttgct
    10621 gcagggaaga acctttacgt tttccgcctt taccgccacg tactgctgcg cgtgcttctt
    10681 cacgatcagc ttttgattca gcgtgtttgt tgcctttctt cggacgcgct gctttcgcgt
    10741 tacgaccacg gccacgaccg ccttcgactt cacgatcgct ttcgtcttct gcctggcgag
    10801 catgttgaga agtagtgacg tgataatcgc tggaatcttc agtcggttcc gcgttatcag
    10861 tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt
    10921 cgagtttacg acgcgcttct tcttcagctt tacgcttgag ctctgcagct tcctgctcac
    10981 ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt
    11041 ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg
    11101 cctcacgttc agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg
    11161 cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca gcctcttgcg
    11221 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    11281 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    11341 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    11401 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    11461 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    11521 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    11581 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    11641 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    11701 tcccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    11761 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    11821 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    11881 tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    11941 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    12001 tcatcacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    12061 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    12121 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    12181 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    12241 gaacacgcgc gccacgcata cctacacagg cacctaccgg atcgatacgt ttatcgttgg
    12301 ttttcaccgc gattttcgca cgagaacccg gatcgcgagc cgctgcttta atttcaatca
    12361 cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    12421 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaatag agcacgccac
    12481 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    12541 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    12601 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    12661 cacgcacttt ctgcacgata acctgttttg ccgtctgggt agtgatacgg tcaaaggtaa
    12721 cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg
    12781 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    12841 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt
    12901 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    12961 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    13021 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    13081 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    13141 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    13201 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    13261 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    13321 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    13381 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgccaacc agttcaaaac
    13441 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    13501 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    13561 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    13621 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    13681 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    13741 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    13801 tactcgtttt gggtaaaaga tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    13861 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaataac
    13921 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttctc ctgctctccc
    13981 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    14041 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    14101 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    14161 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    14221 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctgggccagc cagacgaaga
    14281 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    14341 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    14401 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    14461 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    14521 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    14581 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    14641 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    14701 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    14761 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    14821 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtgg cgctgaacgg
    14881 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    14941 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    15001 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    15061 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    15121 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    15181 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    15241 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    15301 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    15361 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tctcctcctc
    15421 tgccacttca ggcgtgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    15481 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    15541 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    15601 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    15661 ataaggtaat tattgtaagc tgtcgaaaag atgttgtttt ccgttgtgcg atccacgcca
    15721 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    15781 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    15841 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    15901 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    15961 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    16021 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    16081 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    16141 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    16201 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    16261 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    16321 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    16381 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    16441 atacctgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    16501 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg cgtataggta
    16561 gcaaatcgcg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    16621 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    16681 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    16741 aaaaaagcaa aggcacatag ataagggata tacatcccca gcattttgac aacttcatcc
    16801 ggatcactct ggagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    16861 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    16921 agcaggcgaa gcggaaggga tgatatacgt tttacaacca ggatggtcag gacgaagaac
    16981 agcaatcgct gaaccatatg cgcgccggaa gaggcgacta agttcgtaat accaaaaaca
    17041 ataacacaaa gatacaacaa ccaatgagat ttaaaacttc tagcaaattt gttgaatact
    17101 gtcatccaga aatccaagtt cacaaccaat ataaacaatt atcaacaatg ttcaatttta
    17161 tatcagacat gtttttcaac tctgatccat atcagaccga aacactgtta gttttttctt
    17221 tttaaatgat gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa
    17281 acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
    17341 aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg
    17401 tcagcagaag ggaaaagttt ataactaagg cgtaccggca ccatcgtttc aaggtaccag
    17461 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcctc agaccaggac
    17521 caaaacgaaa aaagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
    17581 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
    17641 ccacctcggc acggatacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
    17701 agctggttga gtttgttcgg ttttcaccgg tgcactcaga ttttcccatt cgctaccttt
    17761 attggttttg ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc
    17821 cagcagcgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
    17881 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
    17941 cagaccaaca aggccaattg ccacaataag gaaaactact aaaagagctt cgtacataat
    18001 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
    18061 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
    18121 aattttatcg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgctgaga
    18181 aaaaaggcga caatcgccgc ctttttagcc agttatctaa cgctttaaac ggcttttact
    18241 gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc
    18301 acgcgaatta acggttcggt gccggattta cgcagcaaca cgcgtccacg gttgcccagc
    18361 gcagcttcaa cctctgcggt cacggcttta actgactcat gctcaagtgg atcgccgcta
    18421 cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca ttttcatgcc gctgcaaagg
    18481 tcgtgcaggc tcatatggtt acgtgccatc gccgccagca cctgcaagcc agcaacgatg
    18541 ccgtcaccgg tagtagtttt atccagtagg atcacatgac cggaattctc tgcaccgata
    18601 cgccagcctt tctcctgcat tttttccagt acgtagcggt cgcccacttt cgcgcgcgca
    18661 aatggaattc ctaactgttt cagcgccagt tcaagcccca tgttgctcat caatgtaccc
    18721 acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
    18781 tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccatcgccg
    18841 tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag cgcgcgaacg
    18901 tcggtagccc ccacttcggc attgatgttt acaccgtttg gctcacaacc gatagcgata
    18961 acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca
    19021 caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa
    19081 aactcgatat agcgacccgc ggcatcaacg atacggctgg ctttacccag ttctgccgaa
    19141 tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc
    19201 agtttggtgc cgtcgataga gaagaattta atgccattat cgtagaacgg gttatgagat
    19261 gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
    19321 gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt
    19381 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag
    19441 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggtgtg
    19501 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
    19561 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
    19621 ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca
    19681 ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga
    19741 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
    19801 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
    19861 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt
    19921 cacttctgca aagacatcgt catacttcgg agcttcctgc atggtttttg gatttccctg
    19981 catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc
    20041 ggaaagggag cggatatcat taataatgtg agcgccaact ttcgctgact cacggatgac
    20101 ttctggtttg gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
    20161 cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc ctgggcgcgt
    20221 ggactcgcca ccaacgtcaa tgatcgtcgc gccagcattg atcatcagat tcgcatgttt
    20281 caccgcatct atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt
    20341 gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt
    20401 catgatgtta tccctagtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt
    20461 tcggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg
    20521 gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca
    20581 ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg
    20641 cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca
    20701 tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag
    20761 ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata
    20821 tgtttcgctt tcgctacgct acggccgagg aatacttcac cttcttcttc cgcgtacagc
    20881 agtggaccca atttctcaga gaagccccac tgggtcacca tgttacgtgc cagattggtc
    20941 gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccata gatgatctct
    21001 tctgccagac gaccaccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg
    21061 ctgattgcgt cgccttcagg caagaagaaa gtcacaccca gcgcacgacc gcgtgggata
    21121 atcgtcactt tgtgcaccgg atcgtgttcc ggcaccaggc gaccgataat cgcatgaccc
    21181 gcttcgtggt aagccgttga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc
    21241 gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg
    21301 ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag
    21361 aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggggccaa tggtacgcga
    21421 cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat ctggcaagcc gaccacaacc
    21481 tgacggtcga aacggccagg acgcagcagg gccgggtcga gaacgtccgg acggttagtc
    21541 gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg
    21601 ttcagagtct gttcacgttc atcgtgacca ccgcccagac cagcgccacg ctggcggcct
    21661 acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac
    21721 atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag
    21781 atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcaa cgttttaccg
    21841 gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag
    21901 cggctcggct cgcgcagata ctctaccagt tcagcaactt cttcttttgc ttcgtcgcag
    21961 cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc
    22021 ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc
    22081 cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt
    22141 ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtaat
    22201 ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg
    22261 atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag
    22321 gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac
    22381 atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga
    22441 ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt
    22501 tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact
    22561 tcccgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag
    22621 cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg
    22681 ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca
    22741 cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc
    22801 tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa
    22861 tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg
    22921 atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca
    22981 ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca
    23041 agtttaaacc aggcacggga acgtaacccc tttttctgtg cctgttgaac atatttatcg
    23101 ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa
    23161 ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt
    23221 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
    23281 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
    23341 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
    23401 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac
    23461 cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
    23521 aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac gctaagatta
    23581 tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa
    23641 aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg cctttttcct ttctttacaa
    23701 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa
    23761 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
    23821 gccacgagca ataggcgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
    23881 gccaacgatg cgataagtct gttcttcgtc agaatccaga ttcagcaccg ttacggtagc
    23941 accgaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgttcga
    24001 cagcttggct tcgatgtctt taatacggcc ttcgcagaaa ccctgctgtt cacgagctgc
    24061 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
    24121 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
    24181 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
    24241 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
    24301 caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatagtc
    24361 attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
    24421 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
    24481 attgaccagc tgtatagcgt tcagtgttca ggccgcgaat gttgatgagt acgttactca
    24541 actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc
    24601 tattgattac cacagtcagc agatggcgct gcccgccagt acgcagaaag tgattactgc
    24661 gctggcggcg ttgattcaac tcggccccga ttttcgtttc accacgacgc ttgaaaccaa
    24721 aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc
    24781 gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa
    24841 ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc
    24901 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgccg ctgccatagt
    24961 tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aaacctggtg atatggcttt
    25021 tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg
    25081 aggttctgcc gaagcgcaat actgcgaact ggatgtcgta ccaggcgacc tgaaccgctt
    25141 tacgctgacg ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttgccgtgca
    25201 ggatggagcc agctatgccg gggcaattct gaaagatgag ttaaaacagg caggtatcac
    25261 ctggagcgga acactgctgc gccagactca ggttaacgaa cctggaacgg tagttgccag
    25321 taaacagtcg gccccgttgc acgatctgct taagattatg ctgaaaaagt cggacaacat
    25381 gatcgccgat acggttttcc gcatgatagg ccatgcgcgc ttcaatgtgc ctggaacatg
    25441 gcgggccggg tcggacgccg tgcgtcagat cctgcgccag caagccggtg tcgatattgg
    25501 aaacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac
    25561 catgatgcag gtgctgcaat acattgccca acacgacaat gaacttaact ttatctccat
    25621 gctgccgctt gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
    25681 cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
    25741 attcattacc acagcgagcg ggcaacgaat ggcgtttgta caatatcttt ctggctatgc
    25801 agtagaaccg gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt
    25861 gtataaagat atttatcaga acaattagtc aaaaagaaac cccggcacat ggccggggtt
    25921 ttgctgatta acgcttgtaa atgaactcaa cgccttcttc gtcgtcttcg tcccagtcat
    25981 catcccagtc ctcatcatcc tcttcagcaa tctcttcaag ctgctggcga tgataatcat
    26041 cccacatgaa ttcgactttc tctggctgtt tcgcttcttc agcctgcacg accgggtttt
    26101 caatgataaa ggtcatcaca tcccagcaga gatctttcac gcccagtcca ctcgccgcag
    26161 agatcagata atatttatct tcccagccca gcgcttcagc aatcgctttc gctttctctt
    26221 cagcttctgc cttatccagc agatcgatct tattgaacac taaccaacgc ggtttcgccg
    26281 ccagatcctg gctgtatttt tccagctcgc tgataataat acgcgcgttt tcaaccggat
    26341 cggtgccgtc aatcggatcg atatcgatga ggtgcaacag gacgcggcaa cgttccaggt
    26401 gcttcaggaa gcggataccc agacctgcgc cttccgcagc gccttcaatc agtcctggaa
    26461 tatcggcaac aacgaagctc ttttcgttgt ccattcgtac cacacccaga cttggcacca
    26521 gagtggtaaa cggataatcc gccactttcg gtttagccgc cgataccgcg cgaataaagg
    26581 tcgatttacc cgcgtttggc atccccaaca taccgacgtc agccagcagc atcagctcca
    26641 gcagcagctc acgcttatca cccggcgtac cgttggtttt ctgccgtggt gtacggttaa
    26701 cggacgattt gaaacgggta ttgcccagac cgtgccagcc gcccttagca accagcagac
    26761 gctgaccgtg tttagtcata tcgcccatgg tttcaccagt accttggtcg attatacgcg
    26821 tacctaccgg cactttaatc gtcacgtctt taccgcgctt accggtacaa tcgcggcttg
    26881 cgccgttctg accgcgctct gcacggaaag atttttcaaa acgataatcg ataagcgtgt
    26941 tcaggttttc gtcggcttcc atccatacgt caccaccatc accaccgtcg ccgccatccg
    27001 ggccgccttt cggaatatac ttttcgcggc ggaagctcac gcaaccatta ccgccatcac
    27061 ctgcaacgac cagaatcgat gcttcatcaa caaacttcat tttattctcc gtaaatcatt
    27121 cgcctgcgcg gggttgcgat accaccgttg tatgcttacg taatccgccc caaatacgat
    27181 gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa cgcaccgaga taacctaaaa
    27241 ggtttaacat cggtctggcg aagaaatcgg gccaggccag tgataaaaga tctgaaaaaa
    27301 acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac ctgcgctgcc tgccagcgag
    27361 ccatcgcttc cgccagggcg ccatatccta ccaatgtatt cagtccgcaa aaaattaaac
    27421 atgcgagctg ccagtggcta agctgcgcta tcactccagg ctttgccaga gggaagagcg
    27481 caattgtaca taaagcgtac agtaaaaaca ggatctgcgg tgaggccagc cgacgcaata
    27541 aaaccttttg cgccacgcca taactcaccc aaaccgtcgc cgcaccgacc ccaaagataa
    27601 ctccccaggt gtaatcggtg agctttgtaa atatctcgac cagactggtg ttaaaaaaca
    27661 tcaccaggcc gctcaggagc atcaatgccc ctacaacctg agtgctgcgc attttctctt
    27721 tcaggataaa tacgctggca accatcatgc caactggcga gagttgccca atcacctgcg
    27781 aagcggtcgg actcaggtat tgcaaggatg agctgaacag gatgaagttc ccaaacagcc
    27841 cggcggtcgc cactgccaac aaaatcaacc agcgtggctt acgaaacacg cgtaatggcg
    27901 gcaacctctt cttcaccgca agaatggcac caaggccaat actcgccatc aagaaacggt
    27961 aaaacacgat tgtcggaggt tccatcacct ccagcacctg cttcattgcg attggcaacg
    28021 ccccccagca aattgctgtg gtgagcgcca aaagaatgcc aatgcctgcc tgctgcttca
    28081 taccgtattc cctgcaaagc gcatttgcgg attaacgtcg caccatgcgc atttttaccg
    28141 gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct ttcatccgtt accgggacgc
    28201 gaaaaactta ttcagcttcg atgctgataa atttacggtt tttcgggcct ttaacttcga
    28261 atttcacttt accgtctgct ttagcaaaca gagtgtggtc acgaccgcaa cctacgttag
    28321 cgccagcgtg gaatttggta ccacgttgac gaacgatgat gctacccgcc agaacggatt
    28381 cgccaccgaa acgcttaacg cccaggcgtt tagcttctga atcgcgaccg ttacgtgtgg
    28441 agccgccagc ctttttatgt gccatttgaa atctctcctc aggtcttagg cgctgatgcc
    28501 agtaattttc acatcagtga accactgacg atggccctgc tgcttacgat agtgtttacg
    28561 acgacgaaac ttaacgattt taactttctc gccacgaccg tgagcaacaa cttcagcttt
    28621 gattacgccg ccatcaacga aaggaacgcc gattttgact tcttcaccgt ttgcgatcat
    28681 cagcacttca gcgaactcaa cagtttcgcc agttgcgatg tccagctttt ccaggcgaac
    28741 ggtctgacct tcgcttactc ggtgttgttt accaccactt tggaaaaccg cgtacatata
    28801 aaactccgct tccgcgcaca ccttttcaat gattcagagt gcgctataaa tattcacaat
    28861 agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt gctacagtca atacacgaag
    28921 aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca ttttttcagt acaatcactc
    28981 tatattccta accataaacc ctaagttgcc tttgttcaca gtaaggtaat cggggcgaaa
    29041 agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt taaccgcgca agatatggcg
    29101 ggtgttaatg cggcaatcct tgagcagctt aattccgacg tccagctgat caatcagtta
    29161 ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc cgatgattgc tgtactggct
    29221 gcacgagctg ttggctatga gggaaatgcg catgtcacca ttgctgccct gatcgagttt
    29281 atccacacgg cgactctgct gcacgacgac gttgtggatg aatcagatat gcgcaggggt
    29341 aaagctaccg ccaacgctgc atttggcaat gccgccagcg tgctggtagg cgattttatt
    29401 tatacccgcg cgttccagat gatgaccagc ctcggttcgc tcaaagtgct ggaagtcatg
    29461 tcagaagccg taaacgtcat cgcagaaggt gaagttctgc aactgatgaa cgttaacgat
    29521 ccggacatca ctgaagaaaa ctacatgcgc gttatctata gcaaaaccgc gcgtctgttt
    29581 gaggctgccg cgcagtgttc cgggattctg gctggctgta cgccggaaga ggagaaaggc
    29641 ctgcaggatt atgggcgcta tctcggcact gctttccagt tgatcgacga tttactcgat
    29701 tacaatgccg atggcgaaca gttaggtaaa aatgtcggcg acgatctgaa cgaaggtaaa
    29761 ccgacgctgc cgctgctgca tgcgatgcat catggcacac cagaacaggc acagatgatc
    29821 cgtaccgcca tcgaacaggg taacggtcgc catcttctgg aaccggttct ggaagcaatg
    29881 aacgcttgtg gatctcttga atggacgcgt cagcgtgccg aggaagaagc agacaaagcc
    29941 atcgcagcgt tacaggtgct cccggacacc ccttggcgag aagcactcat cggcctcgcg
    30001 cacatcgctg ttcaacgcga tcgttaatcc cctcccctca tcccgcgcag cgcgcggggt
    30061 ggttccagta aattccataa aaacacttac tcagctctat aacacctgaa aaacaggctg
    30121 tgcatcttta tatattctga atattcacac tccttacagg aactttttag agcaatcggc
    30181 catcaggagt atagtgatgc tcgacagaag aagtgttctg aatgaaagcg actaacttaa
    30241 ggagtgagga aagatggaaa gtaatttcat tgactggcat cccgctgaca tcattgcggg
    30301 tttgcgcaag aagggaacgt caatggcggc ggagtctcgc agaaatggtt tgagttcctc
    30361 aacgctggcg aatgcattat cgcgcccatg gccgaaagga gagatgatta ttgcgaaagc
    30421 cctgggaact gacccctggg ttatctggcc atcacgctac catgatccgc agacccatga
    30481 gtttatcgac agaacgcagt tgatgcgtag ctacactaaa ccgaaaaaat gagtggtctg
    30541 gcggtagccc cgcgaacggg gctgccagct ctcagacgat tactcgcctt tcacacgctc
    30601 aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt tcgtagccac gatcgatgtg
    30661 ataaatacga tcaaccaccg tcgtcccttc cgcaatacag ccagccagca ccaggcttgc
    30721 tgatgcacgc agatcggttg ccataacctg tgcgccagaa agtttttcaa caccgtgaca
    30781 aataacggta ttgctttcga tttcggcatg cgcgcccata cggctaagct ccggcacatg
    30841 cataaagcgg ttttcaaaga ccgtttcggt gatgaatccg gtcccttctg ccaccaggtt
    30901 caacagcgtg aactgggcct gcatatcggt cgggaatgcc ggatgcggcg cggtacgtac
    30961 gttaacagcc ttcggacgtt tgccatgcat atccaggcta atccagtctt cgccgacttc
    31021 gatgtccgct ccagcgtcac gcagtttcgc cagcacggcg tcgagagtat ctggctgcgc
    31081 gttacggcag ataattttgc cgcgagaaat cgccgccgcc accaggaaag taccggtttc
    31141 gatacgatcc ggtagcacgc gatagacacc gccgcctaaa cgttccacac cttcgatgac
    31201 gatacgatcg gtgccctgac cgctaatttt cgcacccagc gtaatcagga agttcgcggt
    31261 atcgacgatt tccggttcac gcgctgcgtt ttcaataatc gtggtacctt ccgccagggt
    31321 tgcagcacac atgatggtca ccgttgcgcc aacgctgact ttatccatca cgatatgtgc
    31381 gcctttcaga cgaccatcga cggaagcttt aacgtaacct tcttccagtt tgatggtcgc
    31441 gcctaattgt tcgaggccag aaatgtgtag atcaaccgga cgcgcaccga tcgtacaacc
    31501 gccaggcagt gaaacttgcc cctgaccaaa gcgcgctacc agcggcccca gtgcccagat
    31561 agaagcacgc atggttttaa ccagatcgta aggtgcgcag aatacattaa cgtcgcgggc
    31621 atcaatatgc acagaaccat tacgttctac tttcgcaccc agctggctta gcagcttcat
    31681 tgatgtatcg acgtctttca gtttcgggac gttctggatc tctaccggtt cttccgccag
    31741 tagagcggca aaaaggatag gcagagcagc atttttagcg ccggaaattg tgacttcgcc
    31801 ctggagcttc gttggcccct gaacacgaaa tttatccatt tagtttgttc tcagttaaca
    31861 attcatatcc gctaccggcg aatcgcccat agctcaaaag ccgttcagtt tgcgatcgcg
    31921 cgcccactcc gcaggggtat acgctttgat cgacacagca tgaatgcggt tatccgcaat
    31981 atattccatc agcgggccat agaccgtctg ctgtttttta acccgactca tgccgtcaaa
    32041 caactcaccc acggcaataa cctgaaagtg gctgccatcg ccggaaacgt ggacttcctg
    32101 gagggagaga gcgttcatca acacgctctg aatttcatta ttttccatgg gatcttcaat
    32161 catcagttaa taaaccagcg aaacatctta gagcaaagtt gcgctggcat aaataagcaa
    32221 aaagcctcgc tgataaatca gacaaggctc gacttgcagg caggtttgcc ggacaggcgg
    32281 ttaacgccat atccggcctg aaaaaattta acgaggcaga acatcagcag gcaaattata
    32341 caatttcgcg agggtataca ctttgtcgtt taccccctga agcgtcacgt tgttgccctg
    32401 ctttttcgcc agatcgataa gatggagcag cagtgccagt ccacccgtat ccacgcggga
    32461 cacacggcta agatcgatgc aggtaatccc cttcaccgct tcctcacgca tttcccaaag
    32521 cggtagcaga acgtcctgat ccagctctcc ggataacgcc agcgtgtcac ccgtctgcat
    32581 ccagctcagt gactcgctca ttattttttc tcttccagag tgattttctg ttgagaaatc
    32641 gatttcagtt gcgcagtcag gccgtcgata cctttggtac gcagcagcgt tccccactcg
    32701 ttttgtttgg tggtgatcat actgacgcct tcagcaatca tgtcgtaagc ctgccagttt
    32761 cccgtctggg agtttttacg ccactggaag tccagacgca ccggcggacg gccattcggg
    32821 tcaataatgg taacgcgaat aggcacaatg gttttatcgc ccagcggctg ttctggcgca
    32881 atctgatagg tttgaccgtg atacatcgcc agcgcctgac cgtaagcctg cttcaggtac
    32941 tcacggaaag cggcaaagta ggcttcacgt tgagcagggg tcgcactctt gtaatactgg
    33001 cccagcacca gcgcaccggc gtatttcacc tgtacgtatg gcagcagttc ctgatcaaca
    33061 atggtacgca gataatccgg gttggcccga atttgcggtt gctcattctt caggcgatcg
    33121 aacgttttct gcgccgcctc gtccatcagc ttatacggat tggtctggtc tgccgcggtt
    33181 gccgcactca gaggtgcaat caccagcaaa gcgaccatca ttaaacgttt aaacatgcgt
    33241 cggttctcct gaaattattt cgttgtaccc acaggttcag tggtttcatt attacctggc
    33301 gcagcagctg gcgcatcgcc actattctta ttgtcatcgc ctttactacc gtaaaggaac
    33361 tgaccaatga gatcttccag caccatcgca gacttagtgt cctgaattgt atcgccatcc
    33421 ttcaggatag cagtccccag ttccgggtct tcaaaaccga cgtttaatgc cagatattgt
    33481 tcccccagca ggccggaagt acgaatgctc agcgaactgg tatctggaat gtggttataa
    33541 cgttgttcaa tttccagcgt tacgcgtggc agataggttt tcgggtccag cgtaatatcc
    33601 gccacccggc ccacaacaac gccaccaata ctgaccggag agcgcgcttt caggccgcca
    33661 atgttatcga acgtcgcata aagcgtgtag gtcggttcag tacgtatgga cgtcacgttc
    33721 gccgccttca ggcaaacaaa cagcgccgcc agcagtgctg ctaataaaaa gatacccacc
    33781 caaatttcat tttttttcgt ttgcatgaac tcaattccca aacatcaatg cggtcagcac
    33841 aaaatccagc cccagaacag ccagagacga gtggacaacg gtgcgagtgg ttgcccggct
    33901 aatcccggca gacgtcggga tggcgtcgta gccgttaaac aacgaaatcc acgtcaccgt
    33961 gatggcgaac accacgctct taatcagaca gttgaccaga tccatacgcc agtcgacggc
    34021 attttgcatt gccgaccaga agaacccgct atcaatgcct ttccaactga cgccgaccag
    34081 agatccgccc cagatcccca cggcgacgaa aataaccgtc aacagtggta atgaaataac
    34141 cccagcccag aaacggggag aaataacccg acgcagcgga tccaccgcca tcatctccat
    34201 actggagagt tgctctgtag cgcgcatcag gccgatttct gcggttagcg ccgaaccagc
    34261 acgcccggca aacaacaacg cggcaaccac cggccccagt tcacgcagta gcgataacgc
    34321 caccagcata cccagactgg tttccgcact ataagtggtc agaaccagat aaccttgcag
    34381 ccccaacacc attccgatga acacgccaga aaccacaata atcagcatcg acaggacgcc
    34441 gacattatag agctggcgca ccagcagcgg cgcatgtttg cgaaattccg gtttgccgac
    34501 cagcgcattg aataacatta acccggcccg cccgaacgtt ctcagggttt taatcccttt
    34561 atgtccgagc gacgccagcg catttaacag catgagtggc ttaactccct ggtaaaagat
    34621 cagcgtgata atcgccggca ggatagcgga acggaacagg cccgtcagct atcccgtcca
    34681 gaaactgacg tacgcgcgga tcaggattcg cctgcaacgc ctgggcactg ccatgagcga
    34741 caattttttt gtccgccagg atccaggcgt gatccgcaat acttaacact tccggcacat
    34801 cgtgagaaac caccacacaa gtcacgccca gcgcgctgtt cagctcagaa atcagcttca
    34861 ccagtacgcc catggtgatg ggatcttgcc caacaaaagg ttcatcaaac atgatgagat
    34921 ccggctccag cgcaatcgca cgcgccagcg ctgcacgccg cgccatccca ccagaaagtt
    34981 cagaaggcat tagcttagcc gctccacgca gccccacggc ctccagcttc atcatcactg
    35041 tactatgcaa caatggcgcg ggaagttggg tatgttcgcg cagtggatag gcgacgttgt
    35101 caaatacgtt catatcagtg aacaacgccc cggactgaaa taacatgctc atccgtttgc
    35161 gcactgtata caggcgcgaa cgagacatcg ccggaatatt ctcaccatcg aaaaggatct
    35221 caccatgatc tggtgcgatt tgcccgccaa tcagacggag tagcgtcgtt ttaccgatgc
    35281 ccgatggccc catgattgcc gtgatcttcc ctcgcggcac ggtcagggaa atattatcga
    35341 agatgcagcg attgccacgc gtaaaactga catcgcgcat atcgactaaa ttcgccacag
    35401 actgctccat aattcaccct tcgtcttgcg ttgattttct aagcatggcg ctcaatttaa
    35461 ccttgaaccc aacatattta cagaatatta cccgccgtgg ttagcgaaag ctggcatttg
    35521 ttttactttt tagccgcata aagtcaaaat taagaatccg ttacggcttt ctgaaaatct
    35581 tcagcggacc ggcgagtata cctgaagaaa ggatgttaga tgcttttagc tacggcactg
    35641 ttaattgttg gtttactttt ggtcgtttac agtgccgacc gcctggtttt tgccgcgtct
    35701 attctttgcc gaacctttgg catcccgccg ctgatcatcg gcatgacggt ggtcagtatt
    35761 ggtacatcgt taccagaagt catcgtctcg cttgccgcgt ctctgcacga acaacgcgat
    35821 ttagccgttg gtacagccct cggctcaaac attatcaata tattgctgat cctcggtctg
    35881 gctgcgctgg ttcgtccttt taccgtccat tctgatgttc tacgccgtga attaccctta
    35941 atgttgttgg tcagcgtggt ggccggttcc gtactctatg acggacaact tagtcgcagc
    36001 gatggtatct ttctcctctt tctggctgtg ctatggctgc tgttcattgt taaacttgca
    36061 cgtcaggctg aacgtcaggg gactgacagc ctgaccagag agcagcttgc agagctgccg
    36121 cgtgacggcg gattgcccgt cgcgttttta tggctcggca ttgcgcttat catcatgcca
    36181 gtggccacgc ggatggtggt tgataacgcc acggtgctgg cgaattactt tgccatcagc
    36241 gagttgacga tgggtctgac ggcaattgct atcggaacca gcctgccgga actggcaacc
    36301 gcaatagcgg gggttcgcaa aggtgaaaac gacattgctg tcggaaatat cattggcgca
    36361 aacattttta atattgtcat cgtgttgggt ttacccgcgc tgataacgcc aggagagatt
    36421 gatccactgg cgtacagtcg tgactacagc gtgatgttgc tggtgagcat tatttttgcg
    36481 ttgctgtgct ggcggcgctc cccacaaccg ggccgtggtg taggggtatt attaactggc
    36541 ggatttatcg tatggctggc gatgttgtac tggttatcgc caatactcgt tgaataactg
    36601 gaaacgcatt atgtcgcacg tagagttaca accgggtttt gactttcagc aagcaggtaa
    36661 agaagtcctg gcgattgaac gtgaatgcct ggcggagctt gatcaataca tcaatcagaa
    36721 tttcacgctt gcctgtgaaa agatgttctg gtgtaaaggg aaagttgtcg tcatggggat
    36781 ggggaaatcg gggcacatcg ggcgcaaaat ggccgcaacg tttgccagca ccggtacacc
    36841 ttcatttttc gtccatcctg gtgaagccgc gcatggtgat ttaggcatgg tcaccccaca
    36901 ggatgtggtg attgctatct ctaactcagg tgaatccagc gaaatcacgg ccttaattcc
    36961 agtgcttaag cgtcttcacg taccgttaat ctgcatcacc ggtcgcccgg agagcagcat
    37021 ggcgcgcgct gcagatgtgc atctgtgtgt taaagtagcg aaagaagcct gtccgttagg
    37081 gctggcaccg accagcagca ccaccgccac gctggttatg ggcgatgccc tcgctgtcgc
    37141 gctgttaaaa gcacgcggct ttactgctga agattttgcg ctctcacacc caggcggcgc
    37201 actgggtcgt aaacttctgc tgcgcgtaaa cgatattatg catacgggcg atgagatccc
    37261 gcatgttaag aaaacggcca gtctgcgtga cgcattgctg gaagttaccc gcaaaaatct
    37321 tggtatgact gtcatttgcg atgacaatat gatgattgaa ggcatcttta ccgacggtga
    37381 tttacgccgt gtcttcgata tgggcgtgga tgttcgtcag ttaagtattg ccgatgtgat
    37441 gacgccgggg ggaatacgtg tgcgccctgg cattctggcc gttgaggcac tgaacttaat
    37501 gcagtcccgc catatcacct ccgtgatggt tgccgatggc gaccatttac tcggtgtgtt
    37561 acatatgcat gatttactgc gtgcaggcgt agtgtaaaga ttcaaggata aacaacaatg
    37621 agcaaagcag gtgcgtcgct tgcgacctgt tacggccctg tcagcgccga cgttatggca
    37681 aaagcagaga acattcgtct gctgatcctc gacgttgatg gcgtactgtc agatggcctg
    37741 atttatatgg gcaataacgg cgaagagctg aaagcgttca atgttcgtga cggttatggc
    37801 attcgttgtg tgctcacctc tgatattgaa gtcgctatca ttaccgggcg aaaggctaaa
    37861 ctggtagaag atcgttgtgc cacattgggg atcactcact tgtatcaggg gcagtcaaac
    37921 aaactgatcg cctttagcga tttgctggaa aaactggcga ttgccccgga aaatgtggct
    37981 tatgtcggcg atgatctcat cgactggccg gtaatggaaa aagtgggttt aagcgtcgcc
    38041 gtggccgatg cgcatccact gttgatcccg cgcgccgatt acgtgacgcg cattgctggc
    38101 ggtcgtggcg cagtgcgcga agtttgcgac ttattactcc tggcgcaggg caaactggat
    38161 gaagccaaag ggcaatcgat atgagtaaag ccagacgttg ggttatcatt gtgctatcac
    38221 tggcggttct ggtgatgatc ggcattaata tggccgaaaa agacgatacc gcccaggtgg
    38281 tcgtcaacaa caatgatccc acctataaaa gcgagcatac ggacacgctc gtctataacc
    38341 cagaaggggc actaagctat cgattgattg ctcaacacgt tgaatattat tccgatcagg
    38401 ccgtttcgtg gtttacgcag ccggtactta ccacgtttga taaggataaa atcccgacat
    38461 ggtccgtaaa agcagataaa gccaagctga ccaatgaccg gatgctctat ttatatggac
    38521 acgttgaagt caacgcactc gtgccggact ctcaacttcg cagaatcacg acggataacg
    38581 cgcagatcaa tctggtgacg caggatgtta cctctgaaga cctcgtcacg ttatacggaa
    38641 caacatttaa ctccagcggt ctgaaaatgc gcggcaactt acgcagcaag aacgccgagc
    38701 tgattgaaaa ggttagaaca tcctatgaaa ttcaaaacaa acaaactcag ccttaatctt
    38761 gtgcttgcca gctcacttct ggccgccagc attccggcat ttgccgtaac cggagacact
    38821 gatcagccga tccacattga atcggaccag caatctcttg atatgcaagg caacgtggtt
    38881 acctttaccg gtaatgtcat cgtcacccag ggcaccatca aaattaatgc cgacaaagtg
    38941 gttgttaccc gtccgggcgg cgaacaaggt aaagaagtga ttgacggcta cggtaaaccg
    39001 gcaacgttct accagatgca ggacaacggt aaaccggttg aaggtcacgc ttcccagatg
    39061 cactacgaac tggcaaaaga ttttgtcgtt ctgacgggta atgcttatct gcagcaggtt
    39121 gatagcaaca ttaagggcga taagatcact tacctggtaa aagagcagaa aatgcaggct
    39181 ttcagcgaca aaggcaagcg cgtaaccacc gttctggtgc cgtcgcagct gcaggacaaa
    39241 aacaacaaag gccagacccc ggcacagaag aagggtaatt aattcgttat ggcaacatta
    39301 actgcaaaga accttgcaaa agcctataaa ggccgtcgcg tggtagaaga cgtcagcctg
    39361 accgtcaact ccggggaaat tgtcggtctg ctggggccaa acggtgccgg taagaccacc
    39421 actttctata tggttgtagg cattgtgccg cgcgatgcgg gcaacatcat tattgatgat
    39481 gacgatatca gtctgctgcc tctgcatgca cgcgcgcgcc gcggtatcgg ctatctgcca
    39541 caggaagcct ccattttccg tcgcctcagc gtttacgata acctgatggc ggtactgcaa
    39601 attcgtgacg acttgtctgc tgaacaacgt gaagaccgcg cgaacgagct gatggaagag
    39661 tttcacattg agcacctgcg tgacagcatg gggcagtcac tctccggggg tgaacgtcgc
    39721 cgtgtagaaa ttgcccgcgc actggctgcg aatccgaaat ttattctgct cgacgaaccg
    39781 tttgccggtg ttgacccgat ctcggttatc gacattaaac gcatcattga gcacctgcgc
    39841 gacagcggcc tgggcgtgct gatcactgac cacaacgtgc gtgaaacact ggcggtttgt
    39901 gaacgcgctt atatcgtcag tcaggggcat ttgatcgccc acggcacgcc tacagaaatc
    39961 ttacaagacg aacacgttaa gcgtgtatac cttggggaag acttcagact ctgatagggt
    40021 agaagtttgc gacgttttag caggagagta cgattctgaa catgaagcaa ggtttgcaac
    40081 tcaggcttag ccaacaactg gcgatgacgc cacagctcca acaggcaatt cgtctgttgc
    40141 agttgtcgac gctggaactt cagcaggagc tacagcaggc gctggagagt aatccgctgc
    40201 ttgagcaaat cgacactcat gaagaaatcg acacccgcga aacgcaagac agtgaaacgc
    40261 tggacaccgc cgatgcgctc gaacaaaaag agatgccgga agagctgccg ctcgatgcca
    40321 gttgggacac catttacacc gctggtacac catccggcac cagcggtgac tacattgacg
    40381 atgagctgcc ggtctatcag ggcgaaacga cgcagacctt gcaggattac ctgatgtggc
    40441 aggtcgagct gacaccgttt tccgacactg accgcgctat tgctacctct atcgtcgatg
    40501 ccgttgatga caccggttat ctgactgtcc cgctggaaga tattctcgaa agtatgggcg
    40561 atgaagagat cgacatcgac gaggttgaag ccgtccttaa gcggatccaa cggtttgatc
    40621 cggtcggtgt agcggcaaaa gatctgcgtg actgcctgct aatccaactc tcccaattcg
    40681 ataaaaccac gccgtggctg gaagaggcca gattgatcat tagcgatcat ctcgatctgt
    40741 tagccaatca cgacttccgc actttaatgc gcgtcacgcg tctgaaagaa gatgtgctga
    40801 aagaagccgt caatctgatc cagtcgctcg atccgcgccc cgggcagtcg atccagactg
    40861 gcgaacctga gtatgtcatt ccagatgtgc tggtgcgtaa gcataacggt cactggacag
    40921 tagaactcaa cagtgacagc attccgcgtc tgcaaatcaa ccagcactac gcctcgatgt
    40981 gcaataacgc tcgcaatgat ggtgacagcc agtttatccg cagcaatctg caggatgcca
    41041 aatggttgat caagagtctg gaaagccgta acgatacgct actgcgcgtg agtcgctgta
    41101 tcgttgaaca gcagcaagcc ttctttgagc aaggcgaaga atatatgaaa ccgatggtac
    41161 tggccgatat cgcccaggct gtcgaaatgc atgaatcgac gatatctcgc gtgaccacgc
    41221 aaaaatacct gcatagtcca cgaggcattt ttgaactgaa gtatttcttt tccagtcacg
    41281 tcaataccga gggcggcggc gaagcttcct ccacggcgat tcgtgcgctg gtgaagaaat
    41341 taatcgcggc ggaaaaccca gcgaaaccgt tgagcgacag caagttaacc tctttgctgt
    41401 cggaacaagg tatcatggtg gcacgccgca ctgttgcgaa gtaccgagag tctttatcca
    41461 ttccgccgtc aaaccagcgt aaacagctcg tttgacccaa ccgataagga agacactatg
    41521 cagctcaaca ttaccggaaa taacgtcgag atcaccgagg cactgcgcga atttgttaca
    41581 gccaaatttg ccaaacttga gcaatatttt gaccgaatca accaggtcta tgttgttctg
    41641 aaagtggaga aagtcaccca cacctcagat gcaacactgc atgtaaacgg tggtgaaatt
    41701 catgccagcg cggaaggtca ggacatgtac gctgccattg atggcttaat tgataagctg
    41761 gcacgccagt tgaccaaaca taaagataaa ctgaaacaac actaattgtc cgggcaatta
    41821 gcatgtgcat ggcggcctgt tgtgcggcac aacgggccat ttgtacggtt aatgctccga
    41881 gcctgttcca ctgtttgagt gggcaggttc ttaggtgaaa ttatgacaaa taatgataca
    41941 actctacagc ttagcagtgt tcttaacagg gaatgtacgc gaagccgcgt ccactgtcag
    42001 agcaaaaaac gcgccctgga aatcatcagc gagctggcgg cgaaacaact tagcctgccg
    42061 cctcaggtgg tttttgaagc tatcctgacg cgggaaaaaa tgggcagtac cggtatcggc
    42121 aatggtattg ccattccgca tggcaaactg gaagaagata ctctgcgcgc cgttggcgtt
    42181 ttcgttcagc tcgaaacgcc tatagctttc gatgccatcg acaaccaacc ggtggactta
    42241 ctttttgccc tgctggtgcc ggcagaccaa actaaaacgc acctgcatac tctgtcgctg
    42301 gtggcgaaac gtctggcgga caaaaccatc tgccgccgtt tacgcgcagc ccagagcgat
    42361 gaagagctgt atcaaatcat tacggatacc gaaggtactc cggatgaagc gtagttattc
    42421 ggtaatgtct cttttagacg ttgtgaggag aaacagtaca tggtactgat gatcgtcagc
    42481 ggacgttcag gttcaggtaa atctgtcgcc ctgcgtgcgc tggaagatat gggtttttac
    42541 tgcgtggata accttcccgt agtgttgtta cccgatctgg ctcgaactct ggccgatcgc
    42601 gagatttctg ccgccgtcag cattgatgtt cgtaatatgc cggagtcacc agaaatattc
    42661 gaacaggcga tgagtaacct gcctgacgct ttctcaccgc aactactgtt cctggatgcc
    42721 gaccgtaata ccttaattcg tcgttacagt gacacgcgcc gactgcatcc gctttccagc
    42781 aaaaacctgt cgctggaaag tgctatcgac aaagaaagcg atttgctgga gcctctgcgt
    42841 tcgcgagcgg atctgattgt cgatacctca gaaatgtccg ttcacgagct ggctgaaatg
    42901 ctgcgtaccc gtctgctggg taaacgtgaa cgcgaactga ccatggtctt tgagtctttc
    42961 ggcttcaaac acggtatccc tatcgatgca gattacgtct ttgacgtgcg cttcttgccg
    43021 aacccgcact gggatccgaa actgcgtcca atgacaggtc ttgataaacc tgtcgccgcg
    43081 ttcctcgacc gccacacaga agtacacaat tttatctacc agacgcgaag ctatcttgaa
    43141 ctatggttac ctatgctgga aaccaacaac cgtagctact tgacggtcgc cattggttgt
    43201 accggcggaa agcaccgttc ggtgtatatt gcagagcaac tggcagacta cttccgctcg
    43261 cgcggtaaaa acgtccagtc acgccatcgt acgctggaaa aacgtaaacc atgaccgtca
    43321 agcaaactgt tgaaatcaca aacaagctgg gcatgcatgc ccggcctgca atgaagctgt
    43381 ttgaattaat gcagggtttt gacgctgaag tgctgttacg taatgacgaa ggcaccgagg
    43441 ccgaagccaa cagcgttatt gcgctgctga tgttggattc tgccaaagga cggcagattg
    43501 aagttgaagc gaccggccca caggaagagg aagcactggc cgccgttatc gccctcttta
    43561 actctggttt tgatgaagat taatcttcat cactttgaca tacaactccc ttcaaactcc
    43621 ccccgacaat aagaaaatca cgtactgaaa tcgttctcaa tcaacgtcat ttgtacattt
    43681 tgtgcgcttt tcactttcag aagaacctta agaaaacctt aagagacatt gtttaggttt
    43741 tgtttaagtt aatcgaccat actggagatc gtcagaaaat atttccagga gatggcatga
    43801 ttcgcttatc tgaacaaagt cccctgggca ccggaagaca tcggaaatgt tatgcgcatc
    43861 cggaagatgc ccaacgctgt atcaagattg tctaccatcg tggcgatggt ggcgataaag
    43921 agatccgccg tgagttaaag tactacgcgc atcttggtcg ccggttaaaa gactggagtg
    43981 gaataccgcg ctatcacggt accgttgaga cggattgcgg aaccggttat gtctacgatg
    44041 tgattgcaga ttttgacggc aaaccctcca ttacgctgac cgaatttgct gaacaatgcc
    44101 gttacgaaga agacattgca caactgcgcc agctactgaa acagctgaag cgttatttgc
    44161 aggataaccg tatcgtgacg atgtcgctga agccgcagaa tatcctctgt caccgcatca
    44221 gtgagtcaga ggttatcccg gtcgtctgtg acaacattgg tgaaagcaca ttgattccac
    44281 tggcgacctg gtcgaagtgg tgttgtctgc gtaagcaaga gcggttatgg aaacgattta
    44341 tcgcccaacc ggcgctggct attgccctgc agaaagatct gcaacctcgt gagagcaaaa
    44401 cgctggccct cacttcgcgc gaagcttaat ccagctggtg ttgttgcata aacggctcac
    44461 cgcctaactg atacatctgc cgtaaaatcc acgcctgacg gctacgcacg tagcccgatg
    44521 gcgaggagac tttgaaacga agtggattag gtaatacagc tgccagtaat gcagcttccg
    44581 accgggtaag tttgctcgcg ggtttgtgga aataacgttg tgccgcagct tcgacgccaa
    44641 acacaccgtc gccaaattcg gcgatattca ggtaaaccgt caggatacgc tttttgctcc
    44701 agactgtttc tatccccagc gttaatccag cttccagccc ttttcgtacc cagctacgcc
    44761 catcccataa aaagagattt ttggccgtct gctgagaaat cgttgaagca ccgcgaatac
    44821 ggttttcatt gcgctcgttg tgcgccaggg ctttctcaat ggaagcgaca tcaaagcccc
    44881 agtgctcagg aaatttctga tcttctgcgg caatcaccgc cagtcccatc cacggcgaga
    44941 tttgatccat actgacccag tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg
    45001 cgctgacctg tcgctcgacc attaccgctg agaagggaac aggcgcaaca ctaaacaacg
    45061 cgatgccccc gccccagaaa acggcgagga caaccattaa ccgcaaaaga aaacggcgaa
    45121 cgaaactaaa caccgttaag cggcttttac tcattcagcc agaaccagca cgcgggaaac
    45181 cagcttatca atgccgctcg ccgcttctgc aatgttctgc gccagcatat atgctggggt
    45241 ggtgacaatc ttattgtctt catcaaccac gatatcatcg acaggacagg gcacatgctc
    45301 cgcgcccatc tcttccagca cttctgcggt atcgatatca gtaccgatgg tcaaacgcag
    45361 cgggaaatcg aaaattttcg gcagcatcgc cggggcaata cacataaaac caagcggttt
    45421 tccggcctga tgcatcgctt gtgccagcgc ctttaattca cggtcaacgg tgcattcgct
    45481 accaagagtg gcaaaattgc ttaaattctt cgccgcgcca aacccccccg gcacaatcaa
    45541 cgcatccagt tcagcggcat cggcctgggc cagaggacgg atttcaccgc gcgttattcg
    45601 tgccgcttca atcagcacgt tgcgcgtttc cgtcatcgct tcgccagtta aatggttgat
    45661 aacatcaacc tgctgcttat ccggtgcaaa gcagaccgcc tgcgcaccgc tgcgtgaaat
    45721 agctaacaac gtcaacaccg cttcatgaat ttcagaaccg tcatagacgc cgcatccgct
    45781 cagaattacg ccaattttct tcattgtgat catcctttcg caattgactg aaacacatta
    45841 atttttttaa taaaaatggt acgcatcaca catttaactg attcatgtaa caaatcattt
    45901 aagttttgct atcttaactg cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat
    45961 cataataatt tacggcgcag ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc
    46021 ggtctagccg gggtcatttt ttagtggctt ttgccaccca cgctttcagc acttctacgt
    46081 cgtgacgcca ctcttctttc atctcttcaa tccattcacc gacgttatct tcccaggcag
    46141 gaaggtccgg agactgaatt tgctgaccca gctgttgcag atggcgtaac cccactgacc
    46201 ccgccgcacc tttaatttta tgtccttcct ctacaatgcc ttttttatcc tgcgccgtca
    46261 gattcgactc cagcacgctg acatagcccg gcatcatctt ctcaaacacc gctaacccgt
    46321 cggtgatcag cttcggtcct acaagttcga gatactgttc cagcatggga atatcgagca
    46381 atgcttctga tttactgttc tcttctgtcg tcaccgtact ctcctcatca tcctgggtat
    46441 cccagaattt cttgatcatc gcggttagcg ccggaaccga aagcggctta ctcagcacat
    46501 catccattcc agcattgagg tactcttgtt tgtctttcag cacgttagcg gttaaggcca
    46561 ccagcggcgg taaatcctcg cgcggataac gcttcgtcag ttcacgagag atatccagcc
    46621 cggtcatatc tggcaactga atatccagca acaccaggtc gtattcgccc ggtttaaaca
    46681 tctccagcgc cgccttgccg gtcatggcga catcaacgct gttacctaat ttttccagca
    46741 cagaacgcgc aacaatcacg ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg
    46801 gtaaaggcat atcgtcttca tcaaacgcat catcgacctc ttctgctacc gacggtgcgt
    46861 ggatcgtcaa cgtaaaggtt gaacctttgc cctgttcgct ggtaaccgta atatcgccgc
    46921 ccatattttt cgccagacga cgagaaacgg ccagaccaat accggtgccg gtggcaggtt
    46981 taccgccatg actgtctttc acctggtaat acatggcgaa aattttatcc agctcatcct
    47041 gcggaatgcc gataccagag tcttccactt caaaatgcag catatcgcct tcatcgtagc
    47101 gcacgcgcac ggtaacctgg ccttgctggg tgaatttgac ggcgttactg atgaggttcc
    47161 acaggatctg ccgtaaacgc gtcccgtcgg taatgacctg atgcggtaat ggcagcgtcg
    47221 gctccaggtt aaagcgcaat cctttttgtt gcgcctgcaa tgcggagaga ttttccagat
    47281 cggcaaggaa gctggtgaaa tcaaccggtt gattatcaag ctggaccttg cgccgttcca
    47341 tcttatccat gtcgataata tcgttaaaga tattccccag cgtgacggcc gaaacatgga
    47401 tggtcttgag atatttttcc tgctcggcgg tgagttcggt atccagcaga atacggctca
    47461 ggccgacgat accgttcagc ggtgtacgca attcgtgact gatggtggag ataaacgtcg
    47521 ttttgtcgcg gctggcccgt tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc
    47581 gaccaaagcc catcaaaccg tgacgtttac ccacgcggtc gtagtacggc actttacgga
    47641 tttcaaagca ggctttgcgc ccgtccgggt aatccagcca ctgttcatag gtcagtgaca
    47701 cattatgacg gaacactttt tcatcggttt caatgacttt tgcggcggct tccggtgagt
    47761 aaacatcagc aggtttcagg tgaaccagtt gtttttcgct ttttccggtc agcagctcca
    47821 tcgcgcggtt acagccggaa aactctttat cttcgttacg ataaaaaacc aggtcgggtg
    47881 aagcatcaag gaaggaacgt aagaatgagg attgctgctc gagctgaatt tgtgtctctt
    47941 cgcgctcttt gatttcaatt ttcagttggc cgaaggtttc ctgcagttct gcttccgctt
    48001 tttcacgaac ggcgatttcc tgatttagct gggcaatatt atcttttaac tgaacgttga
    48061 ggctcaaatc gcgctcgcgc atctcctcca gtttttgcac cagccgtgac agacgttgtc
    48121 gtgactcctc cagttgctcg acgaccaccg atagaaagta gaccgcccac ggcgtaatca
    48181 gcaaaccaaa gaagatagaa cgaataacat caatgctttc gacctgacca tgcagcacca
    48241 tggttaccgc catttgtacc acaatggcaa gaacgacgag ggccagcgcc agcaacattg
    48301 agaagcgcac cagacctaac ttcatcatca ggtcaacata atactgcgcc agcagacgaa
    48361 tttgcttcat tagggaattc cttcacgaca acctgcccca ataataccca attctgacag
    48421 ttacgttata aattgtgtga gaaatgcgga attcccctca acgacctact ccgttggagg
    48481 tatccacgga cgttccagcg ccgatccctg tacgccatgt tcattcagat agcgatccag
    48541 ctcgaccatt cccgtccatc gattttcgca ccacagagga gcaagcagcg tcgggcgacg
    48601 ggcgctggcg gaaatgcggt ggtagatcac ttccggtggc gtatggcgaa tcatttctcc
    48661 ggcagtgagc gtgtaatcct ccagttcaat accgttcaaa cgccccgctt cccaggcttt
    48721 cgccataatg ctgcctttca caatatgcag cggatgcagc tttatgccgt ccacgccggt
    48781 ttcaaccact cgctccagcg tttgcaaaca ttcagcctgc ccttcgccag gcaggccgac
    48841 aattaagtgg gaacacacct tcagcccgcg ctgacgtgcc agctgggttg tacgctgata
    48901 acaggcaaaa tcatggccgc ggttgatgcg atgcagtgtt ttgtcgtggg cggtttgtag
    48961 ccccagctcc agccacactt cgtaaccctg gtccttatat tcgcaaagca gatccagcac
    49021 cgcatccggc acgcagtccg ggcgggtacc aacacacaaa ccgacaatat tggcctggct
    49081 caccgcctgc tgatacatag aacgcagcac ctgaacttcc gcaaaggtgc tggtatacgc
    49141 ctgaaagtag gccagatagc gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg
    49201 ctcggcaatg gaacgatgct gctgcgcttc atcggcaaac gaggcaacat tacagaatgt
    49261 gcagccacca cgcccgatgg taccgtcacg gttagagcag ctaaaaccgc cgtgcagcgt
    49321 cagcttatgc accttttgcc cataacgacg ggtgagatca ccaccaaaca tattgactaa
    49381 tttctgtaac tgcataatct gatagaccgc gccttgaaaa gaggccaaag cctgccattt
    49441 ttagctcaat tcggcgatga cctggatcaa tcgtcccggc ctgcttttat caactgcata
    49501 atcaatcaaa attaccgaaa tttcatgcat aatcacataa atcacttttg cttatcttgt
    49561 gtcagatttt tttatctcct tatggatttt aggcaaaaac agtagcatga aacgtcatta
    49621 ccaattaagg cagtataaaa tgctggcttt ctcgtcagtt caaggcagga taagggttaa
    49681 cacgccttta tgacagtcag gaattgactg tttctctaac gacttccctt ttagccttaa
    49741 agataaaatc tattttaatt tcagtcattt aataaagaat tttgcgctaa agcacatttc
    49801 tgtaccaata agcttgccat ttgacctgta tcagctttcc cgataagttg gaaatccgct
    49861 ggaagctttc tggatgagca gcctgctcat catatttatg cagtaattga gatcccctct
    49921 tcaccgtatt aaccgatgcg aaaaggacaa caagggggcg aatgcgaggc gcgcgtatga
    49981 cacgcaaacc ccgtcgccac gctctttctg tgcccgtgcg cagcggttcg gaagtggggt
    50041 tcccgcagag cctgggggag gttcacgata tgttgtacga taaatccctt gagagggata
    50101 actgtggttt cggcctgatc gcccacatag aaggcgaacc tagccacaag gtagtgcgta
    50161 ctgcaataca cgcactggcc cgcatgcagc accgtggcgc gattctcgcc gatggtaaaa
    50221 ccggcgacgg ttgcggcttg ctgttacaaa aaccggatcg cttttttcgc atcgttgcgc
    50281 aggagcgcgg ctggcgttta gcaaaaaact acgccgtcgg gatgctattc ctgaataaag
    50341 atcctgaact cgccgctgcc gcacgccgca tcgttgaaga agaactgcaa cgcgaaacct
    50401 tgtcgattgt gggctggcgt gatgtcccca ctaacgaagg cgtgctgggt gaaatcgccc
    50461 tctcctctct gccacgcatt gagcaaattt ttgtgaacgc cccggcaggc tggcgtccgc
    50521 gcgatatgga gcgccgtctg tttatcgccc gccgccgcat tgaaaagcgt ctcgaagccg
    50581 acaaagactt ctacgtctgt agcctgtcga atctggtgaa catctataaa ggtctgtgta
    50641 tgccggcgga tctgccgcgc ttttatctgg atcttgcgga cctgcgtctg gaatcggcca
    50701 tctgcctgtt ccaccagcgc ttctccacta acaccgtacc gcgctggccg ctggcgcagc
    50761 cgttccgcta cctggcgcat aacggtgaaa tcaacaccat caccggtaac cgccaatggg
    50821 cgcgtgcgcg tacctataaa ttccagacgc cgcttatccc tgatctgcac gacgccgcac
    50881 cgttcgtcaa cgaaaccggc tctgactcca gttcgatgga taacatgctg gaactgctgc
    50941 tggcaggcgg gatggatatc atccgcgcca tgcgtctatt agtaccaccc gcctggcaga
    51001 acaacccgga tatggacccg gaactgcgtg ccttctttga ctttaactcc atgcatatgg
    51061 agccgtggga tggcccggcg ggcatcgtga tgtccgatgg tcgcttcgct gcctgtaacc
    51121 tcgaccgtaa cggtctgcgt ccggcacgct acgtcattac caaagacaag ctcatcacct
    51181 gcgcctctga agtcggtatc tgggattatc agcctgacga agtggtcgaa aaaggccgcg
    51241 tcgggccagg cgaactgatg gtgatcgaca cccgcagtgg gcgtattctg cactcggcag
    51301 aaaccgatga cgatctgaaa agccgccatc catataaaga gtggatggag aaaaacgtcc
    51361 gccgactggt accgtttgaa gatctgcccg atgaagaagt gggtagccgc gaactggacg
    51421 acgacacgct tgccagctac cagaaacagt ttaactacag cgcggaagag ctggactccg
    51481 taattcgcgt actgggcgaa aacggtcagg aagcggtcgg ttcgatgggc gatgataccc
    51541 cattcgccgt gctctccagt cagccgcgca ttatttacga ctacttccgc cagcagtttg
    51601 cccaggtgac taacccgcca atcgacccgc tgcgtgaagc gcatgttatg tcgctcgcca
    51661 ccagtatcgg tcgtgaaatg aacgtctttt gcgaagcaga gggccaggcg caccgtttaa
    51721 gctttaaatc gccgattctg ctctactccg atttcaaaca gctcacgacg atgaaagagg
    51781 agcactaccg cgcagatacg ctggatatca cctttgacgt cactaaaacc acgctcgaag
    51841 cgacagtcaa agagctgtgc gacaaagccg aaaaaatggt acgtagcggc accgtgctgc
    51901 tggtgctctc cgaccggaat atcgctaaag atcgcctgcc ggttccagcc ccgatggcgg
    51961 ttggcgcgat ccagacccgt ctggtcgatc aaagcctgcg ttgcgatgcc aacatcatcg
    52021 tcgaaaccgc cagcgcccgc gatccgcacc acttcgccgt gttgctgggc ttcggcgcga
    52081 cggctattta tccatacctt gcctatgaaa cgctgggccg cctggtagac acccatgcga
    52141 ttgccaaaga ttatcgtacc gtgatgctca actaccgtaa cggcatcaac aaaggcttgt
    52201 acaaaatcat gtccaaaatg ggcatctcca ccatcgcctc ttaccgctgc tcgaaactgt
    52261 ttgaagcggt cggtctgcac gatgatgtag tgggcctgtg cttccagggg gcggtcagcc
    52321 gcattggtgg ggcgagcttt gaagacttcc agcaggatct gctgaacctg tcgaaacgtg
    52381 cctggctggc gcgtaagccc atcagccagg gcggcctgct gaaatacgtc cacggcggcg
    52441 aataccacgc ttacaacccg gacgtggtgc gcacgctgca acaggcggta caaagcggcg
    52501 agtacagcga ctatcaggaa tacgcgaagc tggttaatga gcgtccggca accacgctgc
    52561 gcgatctgct ggcaattacg ccgggtgaaa acgcggtcaa cattgctgat gttgaaccgg
    52621 caagcgaact gtttaaacgc tttgataccg ccgcgatgtc tatcggcgcg ttaagcccgg
    52681 aagcccacga ggcgctggcg gaagcgatga atagcatcgg cggtaactcg aactccggtg
    52741 aaggcggcga agacccggcg cgctatggca ctaacaaagt gtcgcgcatc aagcaggtgg
    52801 cttctggtcg cttcggcgtt actccggcgt atctggtcaa tgccgacgtc attcagatta
    52861 aagtcgccca gggcgcgaag ccgggcgaag gcggtcagtt gccgggcgac aaagtcactc
    52921 cttacatcgc caaactgcgc tattcggtgc ccggagtgac gctgatctcc ccgccgccgc
    52981 accacgatat ctactctatc gaggacttag cgcagctcat tttcgacctc aagcaggtta
    53041 acccgaaagc gatgatctcc gtgaagctgg tttccgaacc gggcgtaggc accatcgcga
    53101 ctggcgtggc aaaagcttat gccgatttaa tcaccatcgc aggctatgac ggtggcaccg
    53161 gcgcaagccc gctgtcgtca gtgaaatacg caggttgccc gtgggagctg gggcttgttg
    53221 aaacccagca ggcgctggtt gccaatggtc tgcgtcataa gatccgtttg caggtcgatg
    53281 gcggcctgaa aaccggtgtt gatatcatca aagcggcgat tctcggcgca gaaagcttcg
    53341 gcttcggcac tggcccgatg gtggcgctcg gctgtaaata tctacgtatt tgccatctga
    53401 acaactgcgc aacgggtgta gcaactcagg atgacaaact gcgtaagaac cactatcacg
    53461 gcctgccgtt caaggttacc aattactttg aatttattgc ccgtgaaacc cgcgagctga
    53521 tggcacagct gggcgtaacg cgtctggtgg atctgattgg tcgcaccgac ctgctgaaag
    53581 aactggacgg tttcaccgcc aaacagcaaa aactggcgct gtcgaagctg ctggagaccg
    53641 ccgaaccgca tccaggtaag gcactctact gcaccgaaaa caacccaccg tttgataacg
    53701 gcctgctgaa cgcgcagttg ctgcaacagg cgaaaccgtt tgtcgatgaa cgccagagca
    53761 aaaccttctg gttcgatatt cgcaacaccg accgttctgt cggcgcgtcg ctttcaggct
    53821 atatcgccca gacgcacggc gatcaggggc tggcagccga tcctatcaaa gcgtacttca
    53881 acggcaccgc aggccagagt ttcggtgtgt ggaacgcggg cggcgtggaa ctgtacctga
    53941 ccggggatgc caacgactat gtcggtaaag gcatggcggg cggcttaatc gccattcgtc
    54001 ctccggttgg ttccgccttc cgcagccatg aagcaagtat tatcggcaac acctgcctgt
    54061 atggcgcgac cggtggtcgt ctgtatgccg caggccgcgc gggtgaacgt ttcggcgtgc
    54121 ggaactccgg tgctatcacc gtggtagaag gcattggcga caacggctgt gaatatatga
    54181 cgggcggtat tgtctgtatt ctgggtaaaa ccggcgttaa cttcggtgcg ggcatgaccg
    54241 gcggcttcgc ttacgttctc gatgaaagcg gtgatttccg caaacgcgtt aacccggaac
    54301 tggtcgaggt cttaagcgtt gacgatctgg cgatccatga agagcatctg cgcggtctta
    54361 tcaccgagca tgttcagcat actggctctc agcgcggtga agagattctg gcgaactggt
    54421 caaccttcgc cactaaattt gcgctggtta aaccgaagtc cagtgatgta aaagcactgc
    54481 tgggtcatcg tagtcgtagc gcagcagagt tgcgcgtgca ggcgcagtaa ggggtagcaa
    54541 caatgagtca gaatgtttat caatttatcg acctgcagcg cgttgatccg ccaaagaaac
    54601 cgctgaagat ccgcaaaatt gagtttgttg aaatttacga gccgttttcc gaaggccagg
    54661 ccaaagcgca ggctgaccgc tgcctgtcgt gcggcaaccc atactgcgag tggaaatgtc
    54721 cggtacacaa ctacatcccg aactggctga agctcgccaa cgatgggcgt atttttgaag
    54781 cggcggaact gtcgcaccag accaaccccc tgccggaagt ttgcggacga gtctgcccgc
    54841 aagaccgtct gtgcgaaggt tcctgcactc tgaacgatga gtttggcgcg gtgaccatcg
    54901 gcaacattga gcgttatatc aatgataaag cgttcgaaat gggctggcgt ccggatatgt
    54961 ccggcgtgaa gcaaaccggt aaaaaagtgg cgattatcgg cgcaggcccg gcaggtctgg
    55021 cgtgtgcgga tgtcctgacg cgtaacggcg taaaagccgt tgtcttcgac cgtcatccag
    55081 aaattggcgg gctgctgacc ttcggtattc cggccttcaa gctggaaaaa gaggtaatga
    55141 cgcgtcgccg tgaaatcttc accggcatgg gtattgaatt caaactcaat accgaagtgg
    55201 gccgcgacgt gcagctggac gatctgctga gtgattacga tgccgtcttc cttggcgtcg
    55261 ggacttatca gtcaatgcgc ggcgggctgg aaaacgaaga cgccgatggc gtgtacgcag
    55321 cgctgccgtt ccttatcgcc aacaccaaac agttaatggg ctttggtgaa acccgcgacg
    55381 aaccgttcgt cagcatggaa ggcaaacgcg tggtggtcct tggcggtggc gacactgcga
    55441 tggactgcgt gcgtacgtcc gtgcgccagg gagcgaagca cgttacctgt gcctatcgtc
    55501 gtgatgaaga gaacatgccg ggttcccgcc gcgaagtgaa aaacgcgcgg gaagaaggcg
    55561 tagagttcaa attcaacgtc cagccactgg ggattgaagt gaacggtaac ggtaaagtca
    55621 gcggcgtaaa aatggtacgt accgaaatgg gcgaaccgga tgccaaaggc cgtcgccgcg
    55681 cggagatcgt tgcaggttcc gaacatatcg ttccggcaga tgcagtgatc atggcgtttg
    55741 gtttccgtcc acacaacatg gaatggctgg caaaacacag cgtcgagctg gattcacaag
    55801 gccgcatcat cgccccggaa ggcagcgaca acgccttcca gaccagcaac ccgaaaatct
    55861 ttgctggcgg cgatatcgtc cgtggttccg atctggtggt aaccgctatt gccgaaggtc
    55921 gtaaggcggc agacggtatt atgaactggc tggaagttta agctctgtcc cagcgccgcc
    55981 tcctcagaaa agagtcggcg ctgaaattct cctttctttc tggaaagcgc ctcgcataat
    56041 ctgaagaaaa ccaatgtaat acgctcatct ggattgttac cggtagatgc ccggcgtata
    56101 gctgattcca ttattcaacg cagcgccata taaagggaca gataatggaa tcgctctcag
    56161 agagaacctc aacaggctac cagcaaatcc acgacggtat tattcatctg gtcgatagcg
    56221 cccggacgga aacggtacgt agcgttaacg cgctgatgac cgcgacatac tgggaaattg
    56281 gccgacgaat tgtcgaattt gaacaaggtg gcgaggccag ggctgcgtat ggtgcgcagc
    56341 taatcaagcg actatcaaag gatttaagtc taaggtataa gcgtgggttc tctgcaaaaa
    56401 acttacgcca aatgaggctt ttttacctct tttttcaaca tgttgaaatt cgccagacag
    56461 tgtctggcga attaacacct gccctggtcc acctacgtcc gtttactttc cgttaaaaac
    56521 gctgacgccc gcagctttta tgaaaaagag acgctccgct gtggctggtc tgttcgccag
    56581 ctagaacggc aaattgcgac ccaattttat gagcgaacac tactgtcaca tgacaaatca
    56641 gccatgctgc aacaacacgc tcctaccgag acgcatattc ttccgcaaca ggcgatacgc
    56701 gatccctttg tgctcgaatt tctggaattg aaagatgaat attcagaatc tgatttcgag
    56761 gaggcgctga tcaaccacct gatggatttc atgctggaac ttggggatga ttttgccttt
    56821 gttggtcggc agcgaaggtt acgcattgat gacaactggt ttcgtgtcga tctgctgttt
    56881 ttccaccgcc gtttacgctg cctactaatc gtcgatctaa aagtgggcaa attcagctat
    56941 agcgatgccg gacagatgaa tatgtatctc aactacgcca aagagcactg gacgctaccg
    57001 gatgaaaatc cgcccatcgg tctggttctc tgtgcagaga aaggagccgg agaagcacat
    57061 tatgctctgg caggtttgcc taacaccgtt ctggcaagcg aatataagat gcaactacct
    57121 gatgagaaac gactcgcaga tgaactcgtt cgaacacagg cggtgctaga ggaaggctat
    57181 agacgccgtt aatttcagcg gtttcgaatt ctgcagacag tgtctgcaga atctttaaaa
    57241 cgaactaagt tttaagccat taaatcagcc ttaaccttca ccacaacctt tttaatctct
    57301 ccaggctcgc cgacaacgca tcctggttta tgcggttcac ctggcataaa cacggcgaac
    57361 attcccggtt ttaagatgat ggcttgctcg ttatcaatgg tgctgcaaag ctgataatca
    57421 tcctcatggt ggaactcttc acactggcgc gcagtgcctg ccatgccaaa cagaatccgt
    57481 tcctcaccgt ttaataacag ttggatatca atgtattgct cgtgcaattc cgcttttttc
    57541 tcgacgggcg attgagtgtt aaacgtcatg acattcataa agatattgtc gccctgtaat
    57601 tcgtaacgac ccggcgcttt ttcttgcggt ctggcagcta atgccagcgt taacgcgtcc
    57661 tgtaacgcag gatgtaaccc agcagacggt aatgactgta cttcacccat catcataatt
    57721 tttctccctg ggccaacagc gcagccccaa gtaaacctgc atcgtggcgg tagtgcgccg
    57781 ccagtaaatc aacatgaaat gccgctggct cctgcgccag atacgtttcc accagcgcca
    57841 gatacccttc tgccagccca acgctgccac cgaccaccac gcactggcaa tcagttgtgg
    57901 ctttaatatc agcgatcagc cttgcaagcg tacgtgcgga gcggtgaatc agctgctgcg
    57961 cctgctcgtc gccctgcccg gcgcgtgtga aaatagttct cgcatccgcg cccgccagtt
    58021 caccctgcgc tgccgccgca atgccgcgac cagaagcaat cgcctccacg caacccgtgc
    58081 gtccacagcc acagaccggg ccgtgtggat cggcaagcgt atgcccgatg tgccccgcca
    58141 gaccgccagg gccggtaagc agtttgccgc cgctcactac accgccgccg acgccagtgg
    58201 aaacggtgat aaagaccata tcggttatat cgccttccag cgcctgatac tctgcccatg
    58261 ctgcggcctg cgcgtcgtta atggcaatgg tcggcaaatt ggtaagttgt tccagcgttt
    58321 tgactaacgg aaagtgtagc aatccaccga gattatgcgg attcagcgcc agcaaactgc
    58381 cgtcgcgaat aattccggtt gaagcgatgg caacccgctg cgcatgagct tgcaacggag
    58441 agactaatgc ggataaggca tcacgcaagg cttctggtgt ctggctggct ggcgtaggaa
    58501 gttcacgacg atcgcggatc tgcccgtcag cgccaatcag cgcggcggca agtttagtac
    58561 cgccgatatc aatcgccagc gtggtcatag caccgccttt ttcatcgctg tgttgtacca
    58621 ctgacaaatg tgttcaagac gcgtaatggc agaaccgacc gtcaccgccc acgcgccgtg
    58681 gcgcatcgca tctgccgcct gagcaggcgt gttgtaacgc ccttcggcaa tcaccctaca
    58741 tccggcgtcg ctcaacgttt tcaccagcgc cagatccggc tcttctggcg tttcaggcgt
    58801 ggtatagcca gaaagcgtag tgccaataat ttcggctccc agcttttggc atgccaggcc
    58861 gtcttccggc gttgagcagt cggtcatcgc cagtaaaccg tgatggtgaa tacgtgccag
    58921 cagcgtttca acaggcaccg gacgcgggcg gtcggtgccg tcaatggcga taatgtccgc
    58981 gcccgcctgc gccagcgcat caacatcttc aatataggcc gtgatgcgta ccggagaatc
    59041 ctccagatcg cgtttcacaa ttccaataat cggcacgctc accaccgcac gcgtggcttg
    59101 cagatttgcc acaccttcaa tgcgaatggc aaccgcgccc gcctgttctg ccgctaatgc
    59161 catggcggcg acgatttcgg gtttatcgag cgggctgtcc ggaaccggct ggcaggagac
    59221 aatcaggcca ccgttagcag cgattttttg atccagttgt gcaagtaacg acatacatct
    59281 tcccttagcg aaaggcccgg tacatagacc gggcaacagg attaactttt ggttttgact
    59341 aaatcgtttt tggcgctgcc aaatggcacg gcaccgctaa atggtttgcc gtcgatagcg
    59401 tcatgagtac gcaatgcttc cgggcgcagc caacgctgaa cgcgggaagg catatccaga
    59461 ccaatcagca ggatcaccac aaatgtcaga ctgaacgata gcgatgccag cgcagtaccc
    59521 agatccagac gttgagcgat caacgcgccg atgattgggg ccagtgcgcc gcccaatgca
    59581 ccaacgttgt aggtaaagcc caggcccgct gcacgctggt cggtatcgaa ataaccgcca
    59641 atcagtttcg gtaagatccc ggcgatccct tgtccaagca tttgctggaa gaacagtaac
    59701 agaccgagca cccagacgtt tgcgccgcca atcgcaaata ccggaataat caacagctgc
    59761 gaggccaaca ggctacaaac gtacgctttg cgggttccca gccagtcacc gaggaagccg
    59821 ccgacacagc accccaccgc agccccaaag ccgctaaaga acagcacatt ggcgacagta
    59881 tgcgggttat aagccagatc ggttttcaga tacgttggca gcagcgcctg aatcggccat
    59941 gagtagagga aagcaaacaa cacgaccacc atcagcatta cgcccgttgg ccagcgttta
    60001 cccgtacttt gcaccataaa gctgataaag attgcggcgc ataacagccc aagaacagcg
    60061 acgatcgcgg cattttgcag gttaccggcg aagcaaaacc acagcgcagt agccgccgcc
    60121 agtgtcatta cgatattggc aatgcgatgt tcaccacggt agagaatatc taccatcgtg
    60181 cgtactggtg ctttacctgc atgtttctct ttccagtctt ccgcttccgg gatgttttta
    60241 cgcagccaga gagcaaagat gataggcaaa atgccgataa agaacagcgc acgccagccc
    60301 cagaccggaa ccaccaggct atagacctga gcggcaacga cggcccccac agagaagcct
    60361 gaaatcagaa aaccactggc tttgttacgc agatgttttg gccagctttc aatgacatag
    60421 gtggcgctgg aaccgtattc acccgccatc cccatgccga tgaccagacg cgcgataaac
    60481 atggtgatgt agcctggcgc aaagccgcag gccagcgtcc cggccgagaa gagaacgatg
    60541 ctggtgacca ttgccagacg acgcccgtag cggtcaccca tagcgccgag catcaggccg
    60601 ccgaaccagc gagagataaa ggctgcggag atcagacttg ccgcctgcac cgtcgtcagc
    60661 ccgaattcac cttgtacttc ggtgagtacc agggcgatta aaacgaaatc aaaaccgtca
    60721 agcagatatc ccaaccaggc agcggaaaat gcccgccatt gtgcacgatt gagatggcga
    60781 taccacggga tattctgggt tgtagtactc attgtgagtc tcccgcggtg ggcgatgccc
    60841 acacgctttg gtatgaaaat tgtagggtac agatgcgttt atttcccctc acccggtagg
    60901 ggcgagcgag gggaaaacaa ctcacccgcg ctcttgcatc aactgctggg ccagcgcctt
    60961 cagttctggc agatattttt catctaccgg tccaaacggt ttgcggcaca gcggcacaga
    61021 aacgacatcc atataatgga ggacagtttt caggccgcgg aatacgcccg ttttgatcag
    61081 taaatcaatg actttattgc attcagtttg cagtttctgc gcggtctgga tatcgccttc
    61141 tttcagcgcc ttaacgatcc cctgatagcg ccagcccatg atgttgtagg tactaccgat
    61201 accaccatca gcgcccgcca gcagaccaga ggcgaagatt tcgtcgtaac cgttatagag
    61261 cacaagatca ggatgttcac gacggatctg ctccatctga tagagatcgc cagaagtctg
    61321 tttcagcgca ccgacgccag gcaacgtaac cagggtgttg atctgatcca gggtcagttt
    61381 taccccactc agggctggaa tgttgtacac caccatcggc aaaccatccg ctgaatcaat
    61441 aattgcccga tagtgatcgc agtgttcttc aaagctgaaa ggatagtaga acggcgtgac
    61501 ggcggagacg gcatcgaagc cataacgttt agccgatgcc gcaagttgtt ggctttcggc
    61561 ggtgctgacg caaccgacgt gggcgatgag tttaatctta cctttcgcct cttcggcgac
    61621 gatttccagt acctgttcac gctcggaaag gctttgtaca aaggcctcgc cggtcgaacc
    61681 acccacgtat aaaccgtcga tgccctgctg aatattgaac tgaaccaggc gacgcagact
    61741 cgctttatcc agtgcttgtt gttgatcaaa aggagtcagg agtgcagcca ttacgccacg
    61801 taaattcgtt gccataaata cctctgaagt gatgcttgtc tgataaacga tataccttta
    61861 tacctgttat accagatcaa ttaagcaaca ccccatacag aaagcttata atgcgatctg
    61921 cttcactaaa gtggcattat ttctttttgt tggtggtctg accgaaagcg tgccaggtag
    61981 cagagacgct gttgagatgc gattgcaacg cacgatcggc ttcgtcagga tcatgacggc
    62041 ggatcgcatc aacgatcgca atatgctgtt gataactaac gttgttatgt tcgtgcagtg
    62101 cctgatcggt aaccgttggg cgtgcggcaa taagccagtc gagcagggca acgtggatcg
    62161 ccatgaagat tgggttaccg gggatctccg ccagcacgcg gtggaaatca acgtctgaac
    62221 gaatgaatgc cgcgttgtta tccagcgact gactgttaat ttccagtgct tttgccagca
    62281 aatcgatttg ctcatcggtg gcatgttcag ccgcatagcg caccaggctg gattcaaaga
    62341 acagacgtaa ttgttcgaaa tgggcaatcc caccaggatg agaaaggaaa tctttcgcca
    62401 tgccggaaag ctcgccgatg atggtgtccg cagaaggacg tgagacgcgg gcacgttcgc
    62461 cgttgttgat ttgcaccaga cctttgcgtt ttaacgctgc cagcgcttcg cgcacagaag
    62521 gacgcccgac gttaaagaac gccatcagtt cgcgttcaga cggtaattgt tcaccttcgc
    62581 caaattcacg acggcggatc atctgttcca gctcttcttc caccatttcg gagagttttt
    62641 tacgcgccag cgggcggcta cgcaagttgc gaccaattgc aggtgaagaa tcttcggttt
    62701 gcgaatcaaa tgcgttcata aggcccattc tgtaaggtca gtgtgattaa catcatcagt
    62761 gacatcctat cacaggattg aaagtagggg aaaatggcag gtttttctct ttgtgcctca
    62821 tcattaccat aattaacgga ataattaact attgcgaaaa attaatgtaa cgcag
//
LOCUS       DAZGOB010000010        60492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGOB010000010 DAZGOB010000000
VERSION     DAZGOB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.010"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..553)
                     /locus_tag="ACHLIF_000661"
     CDS             complement(<1..553)
                     /locus_tag="ACHLIF_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO9604726.1"
                     /translation="MNSNYMPFTQRDSLGRYYTKESISALLVSQMKAEKVNNIIDLAS
                     GEGSLTYAALDRWKNAEAYSLDIESRMSKKVCDNLTHIVTDALVHSFPEMLARHQGNF
                     DVAVCNPPFTLPEWRDDYFKIISEIGADKYISVSKYVPAEIIFISQVIRFLKKGGEAG
                     IILPDGIFTARKFIGLRRYLLNEH"
     gene            complement(795..2003)
                     /locus_tag="ACHLIF_000662"
     CDS             complement(795..2003)
                     /locus_tag="ACHLIF_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9604727.1"
                     /translation="MKLNARQVDAAKPREKAYKLADGAGLYLEVVPSGSRYWRMKYRF
                     NGKEKRMAFGVYPAVSLAQARALRDDAKKKLAEGIDPSLAKKEEKLVRDVQLNNTFQA
                     VALEWHGTKVSRWSEGYASDIIEAFNKDIFPYIGQQPVNEIKPLVLLNVLRRIESRGA
                     TEKAKKVRQRCSEVFRYAIVTGRAEYNPAADLTSAMSGHESKHYPFLTVEELPDFFKA
                     LAGYTGSPLVVLAARLLILTGVRTGELRGAFWSEFDLEKAVWEIPAERMKMKRPHLVP
                     LSTQALEIVQQLKVMSGQYPLVFPGRNDPRKTMSEASINQVFKRIGYTGRVTGHGFRH
                     TMSTILHEEGFNTAWIETQLAHVDKNAIRGTYNHALYLEGRREMMQWYADYINANHNS
                     CISLLVNATK"
     gene            complement(2167..2242)
                     /locus_tag="ACHLIF_000663"
     tRNA            complement(2167..2242)
                     /locus_tag="ACHLIF_000663"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2207..2209),aa:Thr,seq:cgt)
     gene            complement(2357..3610)
                     /gene="proA"
                     /locus_tag="ACHLIF_000664"
     CDS             complement(2357..3610)
                     /gene="proA"
                     /locus_tag="ACHLIF_000664"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO9604728.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(3622..4725)
                     /gene="proB"
                     /locus_tag="ACHLIF_000665"
     CDS             complement(3622..4725)
                     /gene="proB"
                     /locus_tag="ACHLIF_000665"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO9604729.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            5013..6068
                     /gene="phoE"
                     /locus_tag="ACHLIF_000666"
     CDS             5013..6068
                     /gene="phoE"
                     /locus_tag="ACHLIF_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO9604730.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(6107..6508)
                     /gene="crl"
                     /locus_tag="ACHLIF_000667"
     CDS             complement(6107..6508)
                     /gene="crl"
                     /locus_tag="ACHLIF_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO9604731.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6566..7810)
                     /gene="frsA"
                     /locus_tag="ACHLIF_000668"
     CDS             complement(6566..7810)
                     /gene="frsA"
                     /locus_tag="ACHLIF_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO9604732.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(7902..8360)
                     /gene="gpt"
                     /locus_tag="ACHLIF_000669"
     CDS             complement(7902..8360)
                     /gene="gpt"
                     /locus_tag="ACHLIF_000669"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO9604733.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            8621..10078
                     /gene="pepD"
                     /locus_tag="ACHLIF_000670"
     CDS             8621..10078
                     /gene="pepD"
                     /locus_tag="ACHLIF_000670"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO9604734.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(10135..10749)
                     /gene="prfH"
                     /locus_tag="ACHLIF_000671"
     CDS             complement(10135..10749)
                     /gene="prfH"
                     /locus_tag="ACHLIF_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO9604735.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(10746..11885)
                     /locus_tag="ACHLIF_000672"
     CDS             complement(10746..11885)
                     /locus_tag="ACHLIF_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO9604736.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(12131..12583)
                     /gene="yafP"
                     /locus_tag="ACHLIF_000673"
     CDS             complement(12131..12583)
                     /gene="yafP"
                     /locus_tag="ACHLIF_000673"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9604737.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(12580..13635)
                     /gene="dinB"
                     /locus_tag="ACHLIF_000674"
     CDS             complement(12580..13635)
                     /gene="dinB"
                     /locus_tag="ACHLIF_000674"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO9604738.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(13706..14629)
                     /gene="lafU"
                     /locus_tag="ACHLIF_000675"
     CDS             complement(13706..14629)
                     /gene="lafU"
                     /locus_tag="ACHLIF_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO9604739.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(14632..15495)
                     /gene="motA"
                     /locus_tag="ACHLIF_000676"
     CDS             complement(14632..15495)
                     /gene="motA"
                     /locus_tag="ACHLIF_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO9604740.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(15508..16224)
                     /locus_tag="ACHLIF_000677"
     CDS             complement(15508..16224)
                     /locus_tag="ACHLIF_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO9604741.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(16244..16711)
                     /locus_tag="ACHLIF_000678"
     CDS             complement(16244..16711)
                     /locus_tag="ACHLIF_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO9604742.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(16719..17780)
                     /locus_tag="ACHLIF_000679"
     CDS             complement(16719..17780)
                     /locus_tag="ACHLIF_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO9604743.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(17777..18088)
                     /locus_tag="ACHLIF_000680"
     CDS             complement(17777..18088)
                     /locus_tag="ACHLIF_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFO9604744.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(18093..18485)
                     /gene="fliS"
                     /locus_tag="ACHLIF_000681"
     CDS             complement(18093..18485)
                     /gene="fliS"
                     /locus_tag="ACHLIF_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO9604745.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(18508..19824)
                     /gene="fliD"
                     /locus_tag="ACHLIF_000682"
     CDS             complement(18508..19824)
                     /gene="fliD"
                     /locus_tag="ACHLIF_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO9604746.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(20031..20945)
                     /gene="lafA"
                     /locus_tag="ACHLIF_000683"
     CDS             complement(20031..20945)
                     /gene="lafA"
                     /locus_tag="ACHLIF_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFO9604747.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            21431..22273
                     /locus_tag="ACHLIF_000684"
     CDS             21431..22273
                     /locus_tag="ACHLIF_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFO9604748.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLEKLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     LQKC"
     gene            complement(22328..23305)
                     /locus_tag="ACHLIF_000685"
     CDS             complement(22328..23305)
                     /locus_tag="ACHLIF_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFO9604749.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(23322..24251)
                     /gene="flgL"
                     /locus_tag="ACHLIF_000686"
     CDS             complement(23322..24251)
                     /gene="flgL"
                     /locus_tag="ACHLIF_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO9604750.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            complement(24266..25642)
                     /gene="flgK"
                     /locus_tag="ACHLIF_000687"
     CDS             complement(24266..25642)
                     /gene="flgK"
                     /locus_tag="ACHLIF_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO9604751.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGDLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTSTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PNITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(25824..26123)
                     /locus_tag="ACHLIF_000688"
     CDS             complement(25824..26123)
                     /locus_tag="ACHLIF_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFO9604752.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(26123..27223)
                     /locus_tag="ACHLIF_000689"
     CDS             complement(26123..27223)
                     /locus_tag="ACHLIF_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO9604753.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(27238..27903)
                     /gene="flgH"
                     /locus_tag="ACHLIF_000690"
     CDS             complement(27238..27903)
                     /gene="flgH"
                     /locus_tag="ACHLIF_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO9604754.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADACISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(27986..28771)
                     /gene="flgG"
                     /locus_tag="ACHLIF_000691"
     CDS             complement(27986..28771)
                     /gene="flgG"
                     /locus_tag="ACHLIF_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO9604755.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(28850..29587)
                     /locus_tag="ACHLIF_000692"
     CDS             complement(28850..29587)
                     /locus_tag="ACHLIF_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFO9604756.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(29587..30789)
                     /gene="flgE"
                     /locus_tag="ACHLIF_000693"
     CDS             complement(29587..30789)
                     /gene="flgE"
                     /locus_tag="ACHLIF_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO9604757.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(30813..31526)
                     /locus_tag="ACHLIF_000694"
     CDS             complement(30813..31526)
                     /locus_tag="ACHLIF_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFO9604758.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(31526..31957)
                     /gene="flgC"
                     /locus_tag="ACHLIF_000695"
     CDS             complement(31526..31957)
                     /gene="flgC"
                     /locus_tag="ACHLIF_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO9604759.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(31960..32295)
                     /locus_tag="ACHLIF_000696"
     CDS             complement(31960..32295)
                     /locus_tag="ACHLIF_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFO9604760.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            32377..33114
                     /gene="flgA"
                     /locus_tag="ACHLIF_000697"
     CDS             32377..33114
                     /gene="flgA"
                     /locus_tag="ACHLIF_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO9604761.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            33195..33473
                     /gene="flgM"
                     /locus_tag="ACHLIF_000698"
     CDS             33195..33473
                     /gene="flgM"
                     /locus_tag="ACHLIF_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO9604762.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            33486..33914
                     /gene="flgN"
                     /locus_tag="ACHLIF_000699"
     CDS             33486..33914
                     /gene="flgN"
                     /locus_tag="ACHLIF_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFO9604763.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            34041..34970
                     /locus_tag="ACHLIF_000700"
     CDS             34041..34970
                     /locus_tag="ACHLIF_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFO9604764.1"
                     /translation="MSFIECYEPEYVRKFLARHPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            35149..37593
                     /locus_tag="ACHLIF_000701"
     CDS             35149..37593
                     /locus_tag="ACHLIF_000701"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO9604765.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSLCYQRPGFSDIQKKTTDN
                     IARYFNKLPVTTKPSALPIADTIGFFMETSFHYALYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(37711..38106)
                     /locus_tag="ACHLIF_000702"
     CDS             complement(37711..38106)
                     /locus_tag="ACHLIF_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFO9604766.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(38109..38543)
                     /gene="fliJ"
                     /locus_tag="ACHLIF_000703"
     CDS             complement(38109..38543)
                     /gene="fliJ"
                     /locus_tag="ACHLIF_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO9604767.1"
                     /translation="MRQIIDTLTQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAVSVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(38546..39883)
                     /gene="fliI"
                     /locus_tag="ACHLIF_000704"
     CDS             complement(38546..39883)
                     /gene="fliI"
                     /locus_tag="ACHLIF_000704"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO9604768.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(39876..40586)
                     /gene="fliH"
                     /locus_tag="ACHLIF_000705"
     CDS             complement(39876..40586)
                     /gene="fliH"
                     /locus_tag="ACHLIF_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO9604769.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(40589..41599)
                     /locus_tag="ACHLIF_000706"
     CDS             complement(40589..41599)
                     /locus_tag="ACHLIF_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO9604770.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVGRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(41577..43223)
                     /gene="fliF"
                     /locus_tag="ACHLIF_000707"
     CDS             complement(41577..43223)
                     /gene="fliF"
                     /locus_tag="ACHLIF_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO9604771.1"
                     /translation="MNAQIKKLTLAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(43228..43569)
                     /locus_tag="ACHLIF_000708"
     CDS             complement(43228..43569)
                     /locus_tag="ACHLIF_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO9604772.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(43584..44570)
                     /locus_tag="ACHLIF_000709"
     CDS             complement(43584..44570)
                     /locus_tag="ACHLIF_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFO9604773.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
     gene            44957..45808
                     /locus_tag="ACHLIF_000710"
     CDS             44957..45808
                     /locus_tag="ACHLIF_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO9604774.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            45801..46172
                     /locus_tag="ACHLIF_000711"
     CDS             45801..46172
                     /locus_tag="ACHLIF_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO9604775.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            46169..46921
                     /gene="fliP"
                     /locus_tag="ACHLIF_000712"
     CDS             46169..46921
                     /gene="fliP"
                     /locus_tag="ACHLIF_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO9604776.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            46924..47196
                     /locus_tag="ACHLIF_000713"
     CDS             46924..47196
                     /locus_tag="ACHLIF_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFO9604777.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            47198..47980
                     /gene="fliR"
                     /locus_tag="ACHLIF_000714"
     CDS             47198..47980
                     /gene="fliR"
                     /locus_tag="ACHLIF_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO9604778.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            47970..49109
                     /gene="flhB"
                     /locus_tag="ACHLIF_000715"
     CDS             47970..49109
                     /gene="flhB"
                     /locus_tag="ACHLIF_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFO9604779.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIERLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            49093..51186
                     /gene="flhA"
                     /locus_tag="ACHLIF_000716"
     CDS             49093..51186
                     /gene="flhA"
                     /locus_tag="ACHLIF_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO9604780.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            51291..51569
                     /locus_tag="ACHLIF_000717"
     CDS             51291..51569
                     /locus_tag="ACHLIF_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO9604781.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            51562..51918
                     /locus_tag="ACHLIF_000718"
     CDS             51562..51918
                     /locus_tag="ACHLIF_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO9604782.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(51975..52748)
                     /gene="yafL"
                     /locus_tag="ACHLIF_000719"
     CDS             complement(51975..52748)
                     /gene="yafL"
                     /locus_tag="ACHLIF_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9604783.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            52934..53194
                     /gene="dinJ"
                     /locus_tag="ACHLIF_000720"
     CDS             52934..53194
                     /gene="dinJ"
                     /locus_tag="ACHLIF_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO9604784.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            53182..53469
                     /gene="yafQ"
                     /locus_tag="ACHLIF_000721"
     CDS             53182..53469
                     /gene="yafQ"
                     /locus_tag="ACHLIF_000721"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO9604785.1"
                     /translation="MRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNAL
                     PLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            53625..54365
                     /gene="dpaA"
                     /locus_tag="ACHLIF_000722"
     CDS             53625..54365
                     /gene="dpaA"
                     /locus_tag="ACHLIF_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO9604786.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(54336..55103)
                     /gene="yafJ"
                     /locus_tag="ACHLIF_000723"
     CDS             complement(54336..55103)
                     /gene="yafJ"
                     /locus_tag="ACHLIF_000723"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO9604787.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(55309..55887)
                     /gene="lpcA"
                     /locus_tag="ACHLIF_000724"
     CDS             complement(55309..55887)
                     /gene="lpcA"
                     /locus_tag="ACHLIF_000724"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO9604788.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            56127..58571
                     /gene="fadE"
                     /locus_tag="ACHLIF_000725"
     CDS             56127..58571
                     /gene="fadE"
                     /locus_tag="ACHLIF_000725"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO9604789.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTASLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(58614..59060)
                     /gene="ivy"
                     /locus_tag="ACHLIF_000726"
     CDS             complement(58614..59060)
                     /gene="ivy"
                     /locus_tag="ACHLIF_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO9604790.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            59241..60008
                     /gene="yafV"
                     /locus_tag="ACHLIF_000727"
     CDS             59241..60008
                     /gene="yafV"
                     /locus_tag="ACHLIF_000727"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO9604791.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(60129..60224)
                     /locus_tag="ACHLIF_000728"
                     /pseudo
     CDS             complement(60129..60224)
                     /locus_tag="ACHLIF_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(60232..>60492)
                     /locus_tag="ACHLIF_000729"
     CDS             complement(60232..>60492)
                     /locus_tag="ACHLIF_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO9604792.1"
                     /translation="EKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGI
                     RHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT        13521 a        16445 c        15836 g        14690 t
ORIGIN      
        1 aatgttcatt caataaatat cgtcttaaac ctataaactt tcttgctgta aatataccat
       61 caggtaaaat tattccagcc tcaccgccct ttttaagaaa cctgatgact tgagatataa
      121 atattatctc tgctgggaca tattttgaga cagatatata tttatctgcg ccaatctcac
      181 taatgatttt aaaataatca tccctccatt cagggagagt gaatggagga ttacatactg
      241 caacatcaaa atttccttga tgtcgggcaa gcatttcagg aaatgaatga acaagggcat
      301 ctgttacaat atgagtaaga ttatcacata ctttttttga catccgagat tctatatcca
      361 aagaatatgc ttctgcattc ttccatcggt ctaaagcagc ataggttaag ctcccctcac
      421 cagaagctaa atcaataata ttattgactt tttcagcttt catctgagaa accaataatg
      481 cacttatgga ttcttttgta tagtatcttc cgagactatc tcgttgtgtg aatggcatat
      541 agttactgtt cattttttat actcatctta agtacgctac cgccctaggc gttacggcta
      601 cccaagaacc aacacaatca tgtaatctat tgatatttat aaacttatct tcaaatcttt
      661 cagccctaga acctaaaaaa gatagaagtg taggtaacaa agggcgatcg gatatagggc
      721 taatgtttaa acttaagtat caaaatatac ggttttaggt cattttccta atcaatccta
      781 tcatcacgtc aaatctactt tgtagcgttc accagcaaag aaatacatga attatgatta
      841 gcgtttatat agtccgcata ccactgcatc atctccctac gtccttccaa atacaacgca
      901 tggttatacg tcccacgaat cgcattttta tcaacgtgag ccagctgggt ttcaatccac
      961 gctgtattga aaccttcctc atgcaaaatc gtactcatcg tgtgacggaa accatgcccc
     1021 gttaccctcc ccgtatatcc aatccgctta aaaacctgat taatactcgc ttcactcatc
     1081 gtcttgcggg gatcattacg tcccgggaac accagtggat attgccctga catcactttg
     1141 agttgctgta cgatttccag cgcttgggta gagaggggga caaggtgagg ccgtttcatc
     1201 ttcatacgct cggcaggtat ctcccacacc gctttttcaa gatcaaactc actccagaaa
     1261 gcacctcgga gttcgccagt gcgaactccc gtgaggatca gcagacgtgc cgcaagaaca
     1321 actaacgggc ttcctgtgta gcctgcgaga gctttaaaaa agtctggtaa ctcctcaaca
     1381 gtaaggaagg gataatgctt cgattcatgc cctgacatcg cgctggtaag atccgctgca
     1441 ggattgtatt ccgcacgacc ggttacgatg gcgtaacgaa agacttcgct gcaacgctgg
     1501 cgaaccttct tggccttctc tgtcgcgcca cggctttcaa ttcgacgcag cacattaagc
     1561 agaaccagcg gtttgatttc attcaccggt tgttggccaa tatagggaaa aatatcttta
     1621 ttaaaggctt cgataatgtc cgaggcataa ccttctgacc atcggctcac cttcgttcca
     1681 tgccattcaa gtgccacagc ctgaaacgta ttatttagct gcacatctcg aaccagcttt
     1741 tcttctttct tggcaagcga tggatcgata ccttcggcaa gctttttctt ggcgtcgtca
     1801 cgcagtgccc tcgcttgtgc aagggacact gccggataga caccaaaggc catacgcttc
     1861 tcttttccat tgaagcgata tttcatccgc cagtatctgg aaccagaggg aacaacctca
     1921 agatacaaac cagcaccatc tgccagctta taggctttct ctctgggttt agctgcgtct
     1981 acctgtcgcg cattaagttt cattgggggc atctccctag accgaacaca aaatgccccc
     2041 acttatgccc ccaactgcga cttgatttcg gttgagtcta gttgataaca ggagataaga
     2101 tacgggccag aaaccgcagt atacgagctt ttagttgatt tcggtagact tgggaagagt
     2161 ttgaaatggt gccgataata ggagtcgaac ctacgacctt cgcattacga atgcgctgct
     2221 ctaccaactg agctatatcg gccctgaaag gacatgttca cgaacgtgaa tcacggtgga
     2281 caaggttaaa actaaccggg cgatgcgtca atggccttgt gaatcaaatg gctacttttg
     2341 catcacccgg ttttatttac gcacgaatgg tgtaatcacc aatgccgatc cacttgtaag
     2401 tggtcagtgc ttccagcccc attgggccac gcgcgtggag tttttgtgtg cttaccgcca
     2461 cttccgcacc cagaccaaac tggccgccgt cggtaaaacg cgtagaggcg ttaacgtaaa
     2521 cagcggacga atccacttcg ttaacaaaac gctgggcgtt gcgcatatcg cgggtcagga
     2581 tcgcatcgga gtgttgtgtg ccgtgttcac gaatatgggc gatggcatcg tcaagatcgc
     2641 tgacgatttt gacgttcaaa tctaatgaca gaaactcatc gtcatactct tcggctttaa
     2701 cagcaaccac cttcgcaggg cctgcctgca actgcgccag tgcagctgca tctgcgtgta
     2761 atgtcacgcc gctttccgcc atttgtttgc ttaatgcggg caggaagcta tcggcgatgt
     2821 ttttattcac cagcaacgtt tcaaccgtat tacatgtgct cggacgctga gttttcgcgt
     2881 tgacgatcac ttttaatgct tcagcgatct ctacactttc atcaacgtaa atatggcata
     2941 cgcctatacc acctgtgatc accgggattg tcgactgttc acggcacagt ttatgcaaac
     3001 cagcgccacc acgcgggatc agcatgtcga tgtatttatc catacgcagc atttcactga
     3061 ccagcgcacg gtcaggatta tcaatcgcct gcacggcacc cgccggtaag ccgcaggatt
     3121 tcagggcgtc ctgaatcacc gccaccgttg cagcgttagt gcgacacgtt tctttgccac
     3181 cgcgcaggat caccgcatta ccggttttca ggcacagcga agcgacatca accgtcacgt
     3241 tcgggcgcgc ttcataaatc acgccaataa cccccagcgg tacgcgacga cgctcaagac
     3301 gcaggccgct gtccagtacg ctgccatcga ttacctgccc caccggatcg gcgaggttac
     3361 acacctggcg cacatcatcg gcaatgcctt tcagccgtgc gggcgtcagt gccagacggt
     3421 caagcatcgc ttcgccaagg ccattggcac gcgcgtcagc aacatcctgg gcgttagcgt
     3481 tgaggatgat ttcgctttgt gcttccagtt catcggcgat tttttccagc acgcgatttt
     3541 tttcgcggct ggagagttgc gctaatttat acgaggcttg cttcgcggca atgcccattt
     3601 gttccagcat cagcctgctc cttaacgggt aatcatgtca tcacggtgaa cggcaaccgg
     3661 gccgtattca tatcccagta ttgcatcaat ttcttgcgag tggtgcccgg caatacggcg
     3721 taatgcatcg ctgttgtaac gactgacgcc gtgggcgata tcgcgacctt cgaggttgca
     3781 aatgcggatg acttcaccac gcgagaaatt gccagtcacg cttttaatgc ctttcggcaa
     3841 cagggagctg ccgcgttcaa gaatggcggc agttgcccct tcatctaccg tgatttcacc
     3901 cgccggcggc gcaccgaaaa tccagcgttt acggttttca agcggagtcg cctgggcatg
     3961 gaacagcgta ccgacggaaa tgccttccat cacatcacca ataacgcccg gcttgctgcc
     4021 cgcggcaata atggtgtcga tacccgcacg gcaagccacg tcagcagcct gcaatttggt
     4081 actcatgccg ccagttccga ggcctgaaac gctgtcaccg gcaatcgcgc gcagtgcgtc
     4141 atcaatgccg taaacatctt taatcagttc tgcctgcgga ttgctgcgcg ggtcagcggt
     4201 atacaaacct ttttgatcgg tcagcagcaa cagtttatcg gcacccgcca gaatcgccgc
     4261 cagcgcagaa aggttatcgt tatcgccgac tttaatctct gccgtagcga cagcatcgtt
     4321 ctcattgatt accggaacga tattgttatc gagcaacgca cgcagggtgt cgcgggcgtt
     4381 caggaagcgt tcacggtctt ccatatcagc acgggtcagc agcatttgcc cgacgtgaat
     4441 gccataaatc gaaaacagct gttcccacag ttgaatcagt cgactctgcc ctaccgctgc
     4501 cagcagttgt ttcgaggcga tagtcgctgg cagttccggg taacccaggt gctcacgtcc
     4561 ggcggcgatc gcgcccgacg tcacaataac aatccgatgc ccggcggcat gtaactgcgc
     4621 gcactggcga acaagttcaa cgatatgggc acggttcaga cggcgcgatc cgcctgttag
     4681 cacactggtg ccgagtttta ccaccagcgt ctggctgtca ctcatgattc tctgccattc
     4741 aattttagga aaaatgatat caaacgaacg ttttagcagg actgtcgtcg gttgccaacc
     4801 atctgcgagc aaagcatggc gttttgttgc gcgggatcag caagcctagc ggcagttgtt
     4861 tacgctttta ttacagattt aataaattac cacattttaa gaatattatt aatctgtaat
     4921 atatctttaa caatctcagg ttaaaaactt tcctgttttc aacggggctc tcccgctgaa
     4981 tattcgcgcg ttaattaaaa tcaggaatga aaatgaaaaa gagcactctg gcattagtgg
     5041 tgatgggcat tgtggcatct gcatctgtac aggctgcaga aatatataat aaagacggta
     5101 ataaactgga tgtctatggc aaagttaaag ccatgcatta tatgagtgat aacgccagta
     5161 aagatggcga ccagagttat atccgttttg gttttaaagg tgaaacacaa attaacgatc
     5221 aactgactgg ttatggtcgg tgggaagcgg agtttgccgg aaataaagcg gagagtgata
     5281 ccgcacagca aaaaacgcgt ctcgcttttg ccgggttgaa atataaagat ttgggttctt
     5341 tcgattatgg tcgtaacctg ggcgcgttgt atgacgtgga agcctggacc gatatgttcc
     5401 cggaatttgg tggcgattcc tcggcgcaga ccgacaactt tatgaccaaa cgcgccagcg
     5461 gtctggcgac gtatcggaac accgacttct tcggcgttat cgatggcctg aacttaaccc
     5521 tgcaatatca agggaaaaac gaaaaccgcg acgttaaaaa gcaaaacggc gatggcttcg
     5581 gcacgtcatt gacatatgac tttggcggca gcgatttcgc cattagtggg gcctatacca
     5641 actcagatcg caccaacgag cagaacctgc aaagccgtgg cacaggcaag cgtgcagaag
     5701 catgggctac aggtctgaaa tacgatgcca ataattttta tctggcaact ttttattctg
     5761 aaacacgcaa aatgacgcca ataactggcg gctttgccaa taagacacag aactttgaag
     5821 cggtcgctca ataccagttt gactttggtc tgcgtccatc gctgggttat gtcttatcga
     5881 aagggaaaga tattgaaggt atcggtgatg aagatctggt caattatatc gatgtcgggg
     5941 ctacatatta tttcaacaaa aatatgtcag cgtttgttga ttataaaatc aaccaactgg
     6001 atagcgataa caaattgaat attaataatg atgatattgt cgcggttggc atgacctatc
     6061 agttttaatg attattgccg gatgtgatgc atccggcaca tttcactcac gccgttaact
     6121 tcaccggctc gtcacgaaaa tcatccgccg gttccagctt cagattcagc gtcgtcagca
     6181 gttcacgcag cttctcgtga aactcacgca gggtgtgctc cagtcgttca accacttcag
     6241 tgtcttttac cggaacactc ttccagtcgc ctgctttatc gaacagacca aactggtaac
     6301 tgtaggtaaa acgggattcc tgcgcttcaa gctccatcca ccagccccag aactcacgca
     6361 cttccggtgc cggtttcacg ttgacgcata cagccagaca atcgaaaaag aatcgattat
     6421 cttcgcactt accttcacga atatacgggc ctagtgcggt aaattttttg atcaatctgc
     6481 tcttcgggtg tccactcggt aacgtcattg ctatctcctg ttgtgatgca actgttttac
     6541 caaattggca aaatttagca aatttttaac acaagcgttt ttcgatccag ccggtgattt
     6601 cctgaagacc tttgtcaaaa ttccgataca ccgggttaaa tgggatctct aataatttac
     6661 cgtcagcaga tgatgaggtg attaagcgtg agtcctcttc cgggctgaac ggatcgttct
     6721 tccagtagcc tgataacatt ggcgttgggc agcgacgccc cagcaatcct tgcactttta
     6781 atgaatagcg attcagctcc acgcgtaacg cgtcatcgga agcatcatgc atccccaaac
     6841 gactcgccag aacgtcaaga tacatttccg gcacctgttg ctggcactta aaatcactca
     6901 acagggtatg aactaccgga ccaagacagg caaccgcttt cagacgcggc gattcaagat
     6961 atgccagacg cacggcaacg ttagcgccga aacggaaacc aaaggccgcg acgcgagtgt
     7021 gatccaccca cggtacgtta ggcagcgcct ttaagacgtg ctgatgcaac aggctggagt
     7081 cctgggtgag cttccatttt gaagaaaagc ccaccgatgg catatcaata gtcagcatcg
     7141 caatgccgcg cggcgcaaaa taacgttcat acaggctgta atagtccgtc tgcatcgcat
     7201 ccagaccacc acacattaat actgtcggga acgggccatc gcctttcggc atatgcaaaa
     7261 agccggtgat gggcgcaccg ccgggtacgg taaactccat ctgccgcatc gtgcccggca
     7321 gacgctgggc ggcctcttca taggcgcggt ttgataaagc ctgcgcttgc tcggccaggt
     7381 catctccttt cagatgagga taggcggcaa tgttgtacaa cgtagccgca tgcagccagt
     7441 gtcgaccact gagttgcgga tcttcttccg cacaggcttt ttgttgccac accatcgcct
     7501 gggtggccca ctcataaatc cagttgccac cacggtagcc aatcacagta tcgtataaat
     7561 catcgtcggt acgttcagca tcgctcatca caatacgtgc ctggacgtcg aggatttcac
     7621 gtgggtcaat accgcgccag atccacatca gacggttaat catgcgatac cagtgaggga
     7681 tggttttacc atcaagagcc gactgcacag gcggttgtgc gccgtgatta aagcggcgga
     7741 ctagcgtcga ggtttcagga tgtttaaagc ggggtttgaa cagggtttcg ctcaggtttg
     7801 cctgtgtcat ggatgcagcc tccagaatac ttactggaaa ctattgtaac ctgcctgaag
     7861 ttaaaaagaa caacgcccgg cagtgccagg cgttgaaaag attagcgacc ggagattggc
     7921 gggacgaata cgacgcccat atcccacggc tgttcgatcc aggtatcttg cgggatatca
     7981 acaacatagt catcaaccag cggacgacca gccggttttg cgaagatggt gacaaagtgc
     8041 gcttttggat acatttcacg aatcgcaaca gcagtaccac cggtatccac caggtcatca
     8101 ataacgatga agccttcgcc atcgccttct gcgcgtttca gcactttaag ctcgcgctgg
     8161 ttgtcgtgat cgtagctgga aatacaaacg gtatcgacat gacgaatacc cagttcacgc
     8221 gccagtaacg cacccggtac cagaccgcca cggcttacgg caataatgcc tttccattgt
     8281 tcagaaggca tcagtcggct tgcgagttta cgtgcatgga tctgcaacat gtcccaggtg
     8341 acgatgtatt tttcgctcat gtgaagtgtc ccagcctgtt tatctacggc ttaaaaagtg
     8401 ttcgagggga aaataggttg cgcgagatta tagagatctg gcgcactaaa aaccagtatt
     8461 tcacatgagt ccgcgtcttt ttacgcactg cctctccctg acgcgggata aagtggtatt
     8521 ctcaaacata tctcgcaagc ctgtcttgtg ttgacaacat tttctgctaa ccctgtgacc
     8581 tgcaatactg ttttgcgggt gatcgacaag gagacttaac gtgtctgaac tgtctcaatt
     8641 atctccacag ccgctgtggg atatttttgc caaaatctgt tctattcctc acccgtccta
     8701 tcatgaagag caactcgctg aatacattgt tggttgggca aaagagaaag gtttccatgt
     8761 cgaacgcgat caggtaggta atatcctgat tcgtaaacct gctaccgcag gtatggaaaa
     8821 tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg ccgcagaaaa ataacgacac
     8881 cgtgcatgac ttcacgaaag atcctatcca gccttatatt gatggcgaat gggttaaagc
     8941 gcgcggcacc acgctgggtg cagataacgg cattggtatg gcgtctgcgc tggcggttct
     9001 ggctgacgaa aacgtggttc acggcccgct ggaagtgctg ctgaccatga ccgaagaagc
     9061 cggtatggac ggtgcgttcg gcttacagag caactggttg caggctgata ttctgattaa
     9121 caccgactcc gaagaagaag gtgaaatcta catgggttgt gcggggggta tcgacttcac
     9181 ctccaacctg catttagatc gtgaagcggt tccagctggt tttgaaacct tcaagttaac
     9241 cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc cacgttggcc tgggtaatgc
     9301 caacaaacta ctggtgcgct tcctggcggg tcatgcggaa gaactggatc tgcgccttat
     9361 cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt gaagcctttg cgaccattgc
     9421 tgtcgcagct gataaagtcg acgtcctgaa atctctggtg aatacctatc aggagatcct
     9481 gaaaaacgag ctggcagaga aagagaaaaa tctggccttg ttgctggact ctgtagcgaa
     9541 cgataaagct gccctgattg cgaaatctcg cgataccttt attcgtctgc tgaacgccac
     9601 cccgaacggt gtgatccgca attccgacgt ggcaaaaggt gtggtcgaaa cctccctgaa
     9661 cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt cactgcctga tccgttcact
     9721 gatcgacagc ggtaaagact acgtggtgag catgctggat tcgctgggta aactagctgg
     9781 cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag ccggacgcta attctccggt
     9841 gatgcatctg gtacgtgaaa cctatcagcg tctgttcaac aaaacaccga acatccagat
     9901 tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg tatccggaaa tggacatggt
     9961 ttctatcggg ccaactatca ccggtccaca ctctccggat gagcaagttc acatcgaaag
    10021 cgtaggtcat tactggacgc tgctgactga actgctgaaa gaaattccgg cgaagtaatt
    10081 atttgatttg ctgccggatg gcgtttaatc gccttccggc agtttcatcc ttcattatcc
    10141 ttcgataaaa gccatccctg taaatgtccg tctcgggttg ccacgttcaa tctggtgatg
    10201 gaacattcgc cgcagcgatt tcagcaccgc gctattttcc tgttgctgct gctccagctt
    10261 ccaggcaatc agcaatcgtg ccagccgctt gttagcatgc tgactacgct ctgactgaac
    10321 cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc gccgagtcgg ttttattgac
    10381 atgttgaccg cccggccccg acgaacgcaa cgtctcataa cggattgcat ccgattgttc
    10441 ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag tttttgcgcc catgatgagg
    10501 ccgatacgga ctcggacaaa tccactgaat agtgccacac cagctttcgc ttaatgccca
    10561 tgcgttatcg ccatccagag aaatcagcgc cgaacgcagc gtgtcagagt agtggcccgt
    10621 ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca gcttctttaa ttagcctgtc
    10681 cagcgccttt ttcactgcga gacaacattc ttccggcccc tgcgctgagg agagttgtag
    10741 caagatcatc cttttttccc tccactgttt ttgagcgtta gcaccggacg cagccgcgcg
    10801 acaggaataa ttaaaccggc ctgcaccaga cattgcacca cgctttcagc cgatttatag
    10861 gcttgtggcg cttcttcaaa gatgagttgt ttatcgcgac aaattacccg actgccaagt
    10921 tcagtacgcg agagttgcgt cgccgtgtat ttcgctgcca gacgcccttt acactcggtg
    10981 cgcccccatt tacgccctgc cccatgcgcc agcgagtgga gcgttttttc gttcgcaaca
    11041 ggctgtacaa tccaggagta atcaccacgt gaaccgggaa taatcaccag accgttgtca
    11101 tccggcgtgg cacctttacg atgcaaccag ccctgttgat cgccgatttg acacgcgcta
    11161 acaaagttat gcgccacatc cagaaccgga cttcccgtgg ccttaacttg ttgcattatg
    11221 cgcaaagcga tcatctggcg attaatacac gcaaacgcca gcgcatcgtc atgttccgca
    11281 atataccgta gcgcgtcgtc actgccttga ggcaaaccat gatgcgagaa cgaggcaata
    11341 tgccgctgta aaatagactg ccccaccccc cgcgagccgc tatgaaccaa cagttgcaga
    11401 tgttgtgcat ccagacctgc cagtgcaaac agttcagcgt tgatgatttg atcaacttgt
    11461 tgcagttcag cgaaatgatt gccgccaccg atagagccaa gcgagctgcg ccacggatgc
    11521 tgtgcaaatg ctgacggcag gttttcctcc agccagcttt cttcagcaac gtcatccaga
    11581 tctgataatc gcttttcaaa cttatcggcg ttgtatttgc gagcgagaat atctgtttgc
    11641 catagcgcca taccgcagcc gatatcattg ccgaccagtg ccggataaaa acgaccaaca
    11701 gagaagaacg ctgcgccaat cggatagccg cgtccgggat gtaaatctgg catccccact
    11761 acgcgctgca tgttgggtaa atttgccgtg gtgtgtaatt gttggatcgc taaactctcg
    11821 atccacaggt catcagatgc gatggtaaat accgcatcgg ataagggacg aatataattg
    11881 cccatatacc attccgtaaa tagactcgtc atacttcaag ttgcatgtgt tgcgacttga
    11941 attattgaga gtaacaatta aaaaatcagg aaatggcaga cgaatccagg gcaagaaaaa
    12001 aagaattaat cctggaaagg tctgcaaaga gtcattgata aaggatttgt catgatttac
    12061 ctccttgcag tttgtggaat aagaaaacgc gcacatacta ggcttgcgtt attttcattg
    12121 caagctggat ttaatgttgc tgtttataac gcatataaaa attaataaac cactctcccc
    12181 gacattcaac gcgttgctgc ttaactgtct gaaaaccata acgttcaaaa aagggttttg
    12241 cggttatgct tgcgtccacc gtaagttcgg attcagactt aatcaaaggt tttaacaaag
    12301 cgctggcaac cccacggcgg gtgtattcag ggtcaacaaa taacatatcg atatagtgtt
    12361 caacgcaggt aataaagccg accagttttg cattaatgac tgcaacccgc acttgtgatt
    12421 tcgcgagttt ctccttccag cgagattcgt caatctgcgc ccaggcggca atttgttgtg
    12481 gtgaataatg ctgactggca gtcatcgtaa ccgctctaat gaaaatagcg catagttgct
    12541 gaaaatcgcc aggctgatag tctcttattt gtatgttatt cataatccca gcaccagttg
    12601 tctttccatt tgcgggtcaa gcaacgtcac atgcagcccc accagtcgca caccgcgccc
    12661 gccgcggcgt tcatcccagg ttttacgcgc ggtggcgatt aagtcagctt tattcagccg
    12721 cggccagacg tgctcctgag tggtttgctg aaaatcatca aactttaatt tcactccctg
    12781 acgggcgatc agtaaatcag gttttacctt tgccagacgg cgttcaagtt ccggatacag
    12841 ccgctcgata atcgcttcac attcagacca gtgatgaata tcttccgcca tcgtgcgttc
    12901 cacgccgacg gattttcgta accgttcgct gttaacgtcg cgctcgtcaa tcccctgact
    12961 acgctcccac aaaatgcggc caaatttgcc aaagcgttta agcagcatca ccagatcaca
    13021 cttttgtaca tcaccgcagg tccgcagccc catcgcttcc agttttgccg ccgagacttt
    13081 gccgacgccg gggatttttg ccagcggtaa ggtttgtaaa aatgccggaa cttctgccgg
    13141 cgtaatcaca aactgaccgt tgggtttatt catgtcggag gcgattttgg cgagaaactt
    13201 tactggtgcc acgcccgcag acgctgtcag ttgcagctcg ctgaaaatcg tctggcggat
    13261 ttcctgggcg atgagggtcg cagaaccgtg gcaatggacg ctatcggtga cgtcgagata
    13321 agcctcatcc agtgacaacg gttcaatacg cgaggtgtag cgcgagaaga tttcacggat
    13381 atgatttgag gcttctttgt aggcgtcaaa gcgccccgga agcaaggtga gatgcgggca
    13441 taatttgagc gccatccctg tcggcatagc gctacgtacg ccaaatttac gcgcgggata
    13501 attggcggtg ctgatcaccc cccgacgttc gcggctgccg ccaatagcaa tagggatatc
    13561 gcgcagggcg ggattgtcgc gcatctccac cgctgcgaaa aagcagtcca tatccacatg
    13621 aatgatttta cgcattactc acctctcaac actggtaaag tatacagtga tttcagggtt
    13681 tgagaaatgc gtaaagattc agcatttaac gcgttcgctg cgaaagcacc tgctgcttat
    13741 ccagcttttg caccagcggc tgcaccactt tatccccatg ctgaccaaag tattgataca
    13801 gcgtatcgga cgcacttttg gtcagcacca taatctcaat gcgccggttg cccgcgcttt
    13861 gcggattttt ggaatccagc agcatctggt ccgccattgc gcttacctgc atcactttat
    13921 cttccggcat tccggcctct tccagcaccc gacgagccga aagcgcgcgg tcacccgaaa
    13981 ggttccagtt gttgtagata ttgtttttgt aagccatcgc atcggtatgc ccggtaataa
    14041 taattttgtt atcgagcgag tcgaaaactg gcgcgagctc caccagcagc gttttaaaga
    14101 acggcataat ctgcgcgctg ccgcgttcga acatattgcg attctggtcg tctttaatca
    14161 gtacgcgtaa tccctgggga acaatctcca tctccagatt cgcttccata tgcgcatcac
    14221 gggcaatggt gttgatactg gttgccagct cgccaagctc ggtggcggat ttcttcttca
    14281 gcagcgccgt tttctcattc acatcacgcg cttttttctc cgtctcttcc ggcaccgcca
    14341 cggtggcagg ttttggcgga tgagacgggc tgattttatt taacggcgac agcccgccac
    14401 cgttaaaaat cgactgcccg tgcagcgcgg cgataatgct ttcacgctcg gatttactga
    14461 cgctgttgac aatccacagc gtcataaaca gcgccatcat cgccagggta aagtcggcga
    14521 acgccacctt ccacgccccg ccatgatgcc cggcatgatt ctttttgatc tgccgccgga
    14581 cgatggtggt ttttgcccca cgctcgcggc ggttgcccgt ctttctcatg cttattcttg
    14641 ctccagcatc gcattcaccc acgcgtcgag gttggcgaag gtcggtttgg acgccagatg
    14701 cagcagctta cgcccggcat ccaccgccag cagcgtcggt ttgccgcctg cctgcgccac
    14761 cagcacagtg cgcacgcatt ccagcaaaga atgttctgcc cgcgcctgtt gttccatggc
    14821 gttggcgagc gggtccatca ggcagtagca gataaagacg cctaaaaacg tccccaccag
    14881 cgctgccgcc actttcaggc cgatcagggc aatagagccg tcaatcgact gcatggtgat
    14941 gatgatcccc agcactgctg cacaaatacc aaatcccggc atcgcttcgg cggtacgttg
    15001 cagcgagcgc gacggcgtga gcagcgactc ttccgcagtg tccagttcct gatcgagaat
    15061 gccttccagt tcatgggcgt cgattttacc catcgccatc aggcgaaagt tatcggcaat
    15121 aaaggtcacc agccgcttct gggttaacac cagcgggtat ttctggaaaa tggtgctctc
    15181 ttccggttgc tcaatatgtt gatccagcat gcgcagacca ccgttttgca ccatttcgag
    15241 caactcatac aggcacatta atagctgacg ctgaaactcc ggcccgagct gttttttact
    15301 gatcacacct ttaatttgat gggcaatctc tttcagcaca tgtttcgggt taccaataat
    15361 catcgccccg aaaccggcac ccaaaataat gatcatctcc gccggttgcc agatagccac
    15421 taattgcccg cccgcttcaa aataaccgcc aaatacgcag ccgagcacca ccagtaaacc
    15481 taatattttt tgcatgattt cttcacttta attcggatag acaaaatcac gaattttctg
    15541 cgcgatctgt ttattcagtt gacaaattcg ggcctcggtt agccctaata ccagcgcaat
    15601 ctctttgaga ttcatctcat gctggtaata catggagagg atcagttgct ctttttcgct
    15661 taattgtgaa agcgcctgtt gcagcatttg ctgagtgaca aactgatctt ccagctcccg
    15721 gccaacaagc ggaatgcctg gcccttcgcc gttcagcagt tcgtccaggc tggcgagcgt
    15781 ttccgcccct tccagttgct gatattcgag ataatcttca tgtgagatcc cttccacggc
    15841 cagttctgac cactccggtt cgtgccccag ttttttgcgc gtttcgcgaa tcagatcttt
    15901 catctgatga tatttctgtc gcagctggcg cggtcgccag tcgagagcgc gcagctcatc
    15961 caggatcgcg ccgcgaatac ggtgcacggc atagcccgca aaaccttcgt ccggcagccc
    16021 gtagcggcgg atggcattaa gcagccccat taacgccgtt tgttccatat cctgacgatc
    16081 aataatgcaa ttacactgcg gggcaagttg ccgcaccact ttacgcacca acggtaaata
    16141 ggcctgcaag tagtggctct cctgctgcgg ggttaatacc ggcgcggcag caaattcttc
    16201 tgactcgaat acatcctgta gcatgtttat atccgcctgg tgattactgg aagaccactt
    16261 ttttcaccag caaatcctta tacggtgcgc tcattttgcg acgctgaaga tctttcttta
    16321 acgcgtcaga aatgagtttg cggatggcag atattttcat gccgcgcaaa tcttcaaatt
    16381 tcatttccgt caggcattca accgcaatac tttgatacag cggctcctga tgttttattt
    16441 tttccgcttc tttgtcactg gcgaccacca tcactaattc cgccgacaaa taatggtccg
    16501 cgccgttgtt gtcatgcagc gtgacaatgg tttccggcaa cgtcacaaac acgcttttgc
    16561 tttcatccag ggtttccacc gtttccgtct gcgcggcggc agtcggtttt ccttttccgg
    16621 cataatgatg ccagacaccc catccagctc cggcccccac caccaacgcc agaacggcgc
    16681 tgaccacgcc cgccaccacg attttcttca tattgccttt acactttgat caaaacggat
    16741 tcgttctgtt gaaattgagg cgcatcatca agctgtaagg cggcactcac ctcttcctgc
    16801 tgatggcccg actgttgttg ttgctgcgac tgcccttcgg aggaaacctg tacgttcacc
    16861 tccataaaat tttgcgccgt cagatgctgg cggagatcgt cgctaaactg ctgtaacgcg
    16921 cggcaaactt cactttggtt cgcgccgatg tgcaccatca gtttgccgtt gtcgagttgt
    16981 acggcgatct cgagcttgcc aagcgacggt ggatcgagac ggatggtgga gatctgctgt
    17041 tgtttgttga gctgaaagtg gatctgatct ttcaaaagcg tcgtcagttt ttcgcccagt
    17101 tcgtccatcg aaagcgtggc tggtttggcg acaatctcct gatgatcgct gagcagctcc
    17161 ggcgtcttaa cctgcaacac cgcacgcgca ataaccggtt cactgtccgg cactttttcc
    17221 gttttacgct cgacctgcgg gcgcgcggtc acacgggtta gttcagattt cgggcgagct
    17281 ggctgtgttg agacgcgcgg ctgcgtcggc gtaatggcat gtaaatcttc actagcgtaa
    17341 gtcgccagtc tggcctgctg ttcgggcttc gcctgcggca actgactgat cagttcctgc
    17401 aactgcggcg gcaacggcgt gagttcctgc tgttgcgtct ggcgttgcgg cgcgttttgc
    17461 accaccgctt tcgtcaattg ctggataacc ggcgatgttg cgacgtttcg cggctgcggc
    17521 gtgtcctgat gcggcgcggc aggttgcgga agtaacagcg ccattagcgc ctgcatcgcc
    17581 accggatcgt tctcctgcgg gcgcgtcgcc tgttgctgac tggtttttgc gctatgaacg
    17641 cgctccgcca ccgccgcgac cgccattttc ggcagggtaa acgcgggcgc gttttcaccg
    17701 ctcaccggcg gtaaaatatc cgccttcaac gaagcggttt ccgcaagcgt tccgagcgtg
    17761 gcaagcaatg tcggattcat gacttctccg aaagtaaatt aacaattgag taggccagta
    17821 cgccttcctg atgccgctga tgctgttcca tacgcgcttt caacacttct gcgcgctgct
    17881 cgcgccgttt aatcagggcg ttaaacgcgt catacagtcg cgctttcact gcatccaact
    17941 gcgccgcatc ggtttccgcc gccgcgtaat cgtgaaactg gcgtatcaac tgctgatgca
    18001 gtgccggaag gcgtcgccag cgttgcttgt cgagggcttc attcatcgcc tcgaccagtg
    18061 cgaaaagttt cctgcgttgt ttttccatcg tcttaaccca gcttgcccga aagcccttcc
    18121 cagccttcgc gcaggttgct taagatcagc atcacttcat cgattttctc tgccgacagt
    18181 tcgccgctgg cctcatacaa tcggtaaacg cagtggtcat agagtcgcga aaggttgacc
    18241 accagctcgc cgccagtttc aaactccagc gaactggtga gcgcattgag aatgtcgatg
    18301 catttattga tgctctgggc tttcttctcg aaacgccgcc cttcaatatg gcttttggcg
    18361 cgttccagtt cgtccatcag gccagaaaac agcaccagca ccagttccag aggcgatgcc
    18421 gccgcagtac gcgccgcgag atcgatctct ttatattgcg aatagccgtc ctgtacttgg
    18481 tacatcttta gggatattcc ttctgagtta cgcgaatgcc gccatgctgg ctttcatggt
    18541 gtacacctcg accagcgtgt tggtgtactc ctcgagataa cggtcgtagt tggcgttata
    18601 ggtatcagta agctgatcgc cttcgtcctg aattttgctc atctgatcgt caatgttctg
    18661 ctggcgcagg gtgatgatgc cgttgctgga gtccgtgtag gtgttaataa ggtcgtccat
    18721 ctgcgccacc atgctgttgt cgccaacgaa aatagaggtc agaccgtcag gatttttcgc
    18781 catttcgtcg ttgaactggt cggagtcaat ctgtaagtgg ccgtgagaat cgagggtgat
    18841 gccatagtca acaatcgaca cgccgttgta gctggcatgg gcgatgtcat cgagctggtt
    18901 cgccagtgaa ctgagtccgg cgtcgcctgc aaataccccg gcgctggtac tgtcatcacc
    18961 gtgggtggtt agcgaatcaa ccgtatcaat caaggtgtta taggcatcga caaaggtctg
    19021 tactttctcc tggctggcgc tggaatcttc gctgatgtta aaggtagtga gatcgctgtc
    19081 agaatcgctg acttcggtga aagtcatggt gacaccggga atcacatcat caaacgtatt
    19141 gctgctgttg gtgatctcag gccccgtggc gctgccaaga tgaataatcg cgtcctgagc
    19201 ggaggaaaca tccgtggcaa cgggcgcgct ggtgctatcg ttgctggcat catgccccgt
    19261 tacgctcacc gaaaacgcgc tctgcgcccc ggtgctatcc gaggtgagca taatcgttgt
    19321 ggtgccgtcg gtttttacca gcgccgccga cacgcccgga ttatcatcgg agtcgttaat
    19381 cgcattcacc agttcactgg cgtcgataaa accgtcgccg tcaccattct gatctgccgc
    19441 cgacagatcg atatccatgg tgctgtcgcc catcgtcagt tcaaaagtgc cggtggcgga
    19501 aaaggcgtcg tcgcccatac tgaacgtggt ttgctgcccc tgtgccagtt gctcaacgaa
    19561 aaatgagtag cttcccgcct gcgcctggga attggcggaa acgctcgccg agtcattatt
    19621 gctggtggcg gcaaacgtca ccgggccgtc ggtatcgctg ttcagcgcgt caacggcgct
    19681 ctgaaaatcg ctcaacgctg aactgagcga atccaggccg ctgctttcag catccagctc
    19741 tgtttgcttc tcctgcaagt ttgccgcctg agtggcgaca tccgcatacg cgatttcttg
    19801 cgcaatggtt cttgggttga tcattgatga ctccggataa cgatgtcagg ttattggcaa
    19861 aaggcgtgcc aatatttttt atgtgaataa tcaatagatt aaaaaacagg cggaaatggc
    19921 gtttccggtc tggcggaaaa aagcctgccg cagaaagcac aacaccgccc gcaggcggtg
    19981 ttgttcgtag tccgaataag gcgtttacgc cgcatccggc aagtccgcca ttactgcagc
    20041 aggctggaaa ccatgctgga catgctgttg gactgtttca gcatggtgat gccagtctgc
    20101 atcagcacct gctgtttggt catattggac gcttccgtcg cgtagtcggt atccataatg
    20161 ttaccgatag cgacttcggt gttgtcctgc atactggtca ggttgttggc ggtgtcgttc
    20221 agacggttga tagacgcacc cagcttggac tggatctgcg aaacgtcatc catcgcggta
    20281 gagatgctgg tgatcatctg gttagccgag ccagacgcgg tcagctcagt accgccagaa
    20341 acacctgcag tacccgtggt gtctgcctga tccgcgctaa aggagttgct gatagcagaa
    20401 agatcggtca ccagcgtgtt cagttggctg gagatgttca cggtcatggt gtcggaagat
    20461 tccgcgccca cctggaaagt taccgcgctc tggaacagac cgtcagtacc ggtgttgctg
    20521 gtaccggaaa caccgaacag gttggtgccg ccgtaagtgg tgttttgcag catgtcagac
    20581 atctgctgac ccagttcgtc gtattcgtcc tgcatcgcct gcagatcgtc gtcgctgtag
    20641 gtgccgttcg ctgcctgagt tgacaggtct ttcatacgac ccagcacatc agacatttca
    20701 tcgaacatac tatcggcggt ttgcagcatc gcggttgcgt cattgatgtt gctcagcgcc
    20761 acgcccatac cgctggactg tgctgtcaga cggttagcaa tttgtttgcc tgcggcgtca
    20821 tcggcagaag agttaatccg gttacctgtc gccaggcgtt ccatagatgt agacaaagaa
    20881 gagctgcttt tgctgattgc attaacggcg gccattgagg cgttattggt gttaatagat
    20941 aacatgatac tgctccttgg gattaatcgt tttcgtttcg ataccctgta aaaacgacac
    21001 ctaaggagca aacttaaaac cgccgttaaa aaaatttcac tgcgatttat ctgatgccac
    21061 ttttagctaa aaaaagcaga gtttaaattc agataaaaag attattagca atatctgcca
    21121 catggccagg tatcaaaata tgtctttatc aaacgcttga tatagttaag attaccatca
    21181 attaacgcgt tttcttctca atgtactgat gccaaaattt acattgcgtt gcaaatagta
    21241 cacatttggc tattgtacca gtcctgtcat ttcgttacat ttccatgcca tatagtgatt
    21301 aagaataatc ctattaccat aaaatattag ttatcacgca gaaaaatcat cttctcaggg
    21361 acgtataaga ctgtgcttta tgcaaagcac tatcaggaaa catttatccc ttgctaaggt
    21421 aaactcaact atgagtatta ttatcaacaa ctggcggatg gacccgtcgc ttaatgcctt
    21481 aatacattgc gaaacgggtg aaacacgtcg tctcggcgaa taccatttta ttttgctgga
    21541 aaaattagca aaaaatgccg atgtggtttt atcgcgatct tatttatgtg ccgaagtatg
    21601 gaagaaccgc attgtcggcg gaaacagcct gccgacggcg atccatgcgt tgcgcgttgc
    21661 tatcgatgat gatggcaaac aacagaacat tattaaaaca attccgaaga aaggttactt
    21721 atgcaataaa gagtatgtgt ctttgccgga ctcatcgcct gcaaaaaaac tgattattac
    21781 cgaccagata caggaaacag taccagaaga gatatcttct acaacaccgc ctgtgcctgt
    21841 tagaaaaaaa cataaagtaa taatggggct agcgttagcc gcggctgtta tatttattgg
    21901 ctcgaccgtg gggtattcac acttgaaaag tacacctgat gccccgcaac tggtaaaaga
    21961 gtcaattaac agtccaagaa taaaaatatt tcaccttagt tctggaaaag aaaataattc
    22021 cgtaccgtta ctgtcacaaa ccctcgcacc aggaaaagac aaactcgata atttattgtc
    22081 agcgcataat atgacaatga caacctatta taaatatgtt cgtaaccgac tggaaagtga
    22141 tattgttctg cgcaaccagt gcaatggtag ctggcaatta acttttaatg tggaaggctg
    22201 gcagaacagc gatattaata gcgcgatgta tcagaattta gagaagttgt taaatacttt
    22261 gcagaaatgc tgatgagtgc caatatatgc cggatgcgac gctaaacgcg tcttatccgg
    22321 ctgataatta ccccaataaa ttcctcaccg tctggctgga aattcgcgcc tgaccattca
    22381 ccgcctgcaa taaaacctga tagttatgat tcgcgctatc cagcacgccg ccgaggtttt
    22441 ccgacgcctg caccgcgttt tccgtttgcg ggaagcgcga catgccgtcg atcaactgcc
    22501 gcgccttttc ctgctgtacc gccatctgcg cacgctgctg attaatgctt tctaacgtct
    22561 gagaaattcc cgcaccgccc tgttgcaggc tttgagctaa acggtctgcc tcagacggct
    22621 cggcaaaagt tttcagcggc ataaacgccg acttatcgcc atcggtgcgc acgcttaagg
    22681 tacgttcgat atggggccac tgtttttcgg tggtggagaa actgatgccg tccgcttgct
    22741 gatgtagctg cacgcccacg cggcgcaggg cgttggtcag ccgcgtctga tactgcgcgg
    22801 cgctgtcatc ctcagacagc atcaccgccg aaagctgagt ctgccgcccg tcagagacgc
    22861 tgaacatgcg cgttcccggc gtgggattgt gtaccagatt cgccagatcc ggcgagtgaa
    22921 aggtcacccg cgcttcgccc tgtaacaccg gctgcaactg gcggtccacc gcaccgccgg
    22981 aaagcgcggt gcgtttgtcc agcatctgca tcagcgccgt attctgcgcc tgcccgcctt
    23041 tacgctggct gtgacgatag tcgagcaact gctgctccag ttgcccgaga taatgatccg
    23101 cctgctgcaa cgtggtgagc tggtcgttaa gctgcacgct gtaacgctgc gggcgagtgg
    23161 taatcagcgg cgaggccgga tattcactcg ccgtcgccgg gagtttttgc cgcaccgcct
    23221 gagtgggcgc aacggttgac gacgataccg cagcgctatg cgcgccgccg gtggcgagtg
    23281 atgaagatgt aagtccgacc tgcatactgt tcctgaaaga gttagagaac gctgaacaaa
    23341 ttcaactcac tcactttcga gtaagtcttg agggtaactt ccattgccac ttccagtccg
    23401 gtaaaggtga tcgacgccgg gccgtaatcg agatcctgta atgaaccaat cagctcatca
    23461 ttagaggtgg agatatcggt ctgcgcatcg ctcagcatcg acatggtatt ttgcgtctcg
    23521 ccgagtgacg caatcgaggc gttgaggtcg tccgaggcgg tgtccaccac atcaacggca
    23581 ttctgaatat cgctctgcac ctgcggatct gccgggtcga cgtccgggtt ttgcaactcc
    23641 tgggataggg aattcagggt gttgagcacg tctaaattgc tgccaaagaa atcactcgcc
    23701 gccacgttgg tgtccacttc cacgccgttc gacaccgtgg tctggcggtg gtcgctgtta
    23761 ccctgatagc tgtagctgtc ggtgacgcca tcgccgtcat cgtccactgc cacaatcggc
    23821 ggctgatcgt tgatggtgcc gccaaacacg tagtgcccgt cttcgtcctg ataattgagc
    23881 gccgccacca ttgattcggt gagcgactca atatcctgcc cgaggcttgc gagtgaatcg
    23941 gcggtattgg tgccattcgc cgcttccagc aggtcgtcgc gcaccgccag caggctgttg
    24001 ttgacgccat caaggatcga ctcctgctgg cttaagcccg ccgacgcgga gtcgatattg
    24061 ctctgatact gctcaatcgc cgactgctgg cggtttaact gggtgattcg cgtggcggcg
    24121 atcgggtcat ccgacggctg caaaatctgc ttgccggttg ccatctgctc gacaacatgc
    24181 gccagatcgc cagagagatt attaaaactt tgcgtcatcg agacgtaggt ttgttgggta
    24241 gttactcgca ttttcgcgct cccgcttagc tgcacatttc cagcaccgaa tcgaaaatct
    24301 cggccccggc ggaaatgact ttcagattgg cttcataaat ttgttgatag gtgatcaggt
    24361 tcaccgcctc ttcgtccatg ctgacgccgc tgacgctgct ctgctggttt tgcgctgcgg
    24421 aatagacatt ggacgcggca tcgacttccg tctggttttg ctgactgtaa atgccgatat
    24481 tgctgatgat tgacgagcac gcctgcccga ccgtcacgct gccgaggttg gctatttcca
    24541 gcggttcggt ggagatattg atcagcgcct gcaggttgtc gctgttgccg ctttcgtccg
    24601 gtgaactgga aaacgccagc tcatcggcgg tgatattcgg gttaacggtc agcgggccat
    24661 cggcattgct ggcgtcataa ataaacagcg gctcgccggg gttaccgttg agatcgtagc
    24721 cctgcgccag ctggttgttg accgcatcgg cgaactgcga cgccatgctg ttgatggtgt
    24781 cggtcagcgg cgtcagcaca tcgttttgat aatcaaacag tgcgcctaat gaaccgccgg
    24841 tgtcggtggt catcgtcgag gtggtgccag cgaaggtcag cgacatggtc gacgtgccat
    24901 cggcgttggt ttccagcgcg atggtcgaac tttgctgccc actcaccagc ggctgaccgt
    24961 ttttcagggt aacgttgtag ttgccctggt cgtcgatatt gacctgcaca tccatcatcc
    25021 cgctcagttc ttccaccatc tggtcgcggg cgtcgtacag cgcggaggcg ttatcgccat
    25081 tggcttcagc ctgagcgatt tgctggttat agctggcgat gccgctggtg agcgtattga
    25141 tttgcgacac catcgcctgc tgctggctga tgatttccgt gctttgcgag tcgatgtaat
    25201 ccagcgtgtt atcaatacgc aacgacagcg ccccggcttc actgatcacc tgctcgcgca
    25261 gggcggaatc atcggggctg gtggttgctt cgttaagggc ggcgaagaag ttatcgaagc
    25321 cgccgctcag gctgctgtta tcgtcgctca gtaccgcttc cagttggctg agatccccct
    25381 gctgggtgct gtaatagccg taatcgctgg cggcatacca cacctgattc acctgatact
    25441 ggttggagac gcggcgaatg ctgtcgacct gtacgccgtt accggcgctg ttcggcgaac
    25501 cgccgctggc accgatggtg ctgatctccg ccacctgacg ggtgtagccc gtggtcatgg
    25561 cgttggcggt gttttgcgcc gtgacgttaa gctccacctg cgcggttgat gcaccgctgt
    25621 agccgatgtt aatcatgtcc atgttggatc cttaccggta aatcgtgccg gttaaaagcg
    25681 gcgtaaagtt ggggattctt aagttccgta aactgggtgc cgaacgccgg atgcggcgtg
    25741 gacgccttat ccggcctaca tacgggcgac tcgtaggcct gataagacgc gacagcgtcg
    25801 catcgtgcac cgaacgccga agctcattta tgcttcggcg acaactgctt cacaatcatc
    25861 gccgcaatgc caatcccgtg ctgctgcgcc agggtgttac acaactcatc gtcataaaac
    25921 ccctgcatca tgcgcaccga atcgctgtta aacgggttat cttttgaatc aaacaactcg
    25981 ttggttttcc gcatctcttt cagcatgttg cgtaaaaaaa tcgcctcaaa ctgttcggcg
    26041 gcctgcttga tatcattggc ctgaaccttc ggccccatta acgcgtcgct gccgtcaatc
    26101 ccgccgctgg cgttcacttt catcagatca cctccaggtc ggcatccagc gcgcccgcct
    26161 catgcagcgc ctgcaaaatc gacataatgt cgtccggcgt cgcgcctaaa ctgttcagcg
    26221 cattgacgag cgttttcagg ctgctcgact ccggcaaact caccacgccc ggacgggcat
    26281 gattcaccgc gatattgctc tgcggcgtaa ccgccgtgcg cccacccgca aaggcattcg
    26341 gctggctgac gttgctggtt tcgttaatcg aaacggtcag gctgccgtgc gacaccgccg
    26401 ccgcgtgtag cgcaacgcca tcgcccatta ccaccgtgcc ggtacgcgag ttaaacacca
    26461 cgcgagcgcg gattttctcc gcctgcacct gcacatcgtc cagttgcgac ataaatgcca
    26521 cccgcgcgcc ggggctggtg ggcgcgcgca cggtaacgtt ggtggaactc tgcgcggtgg
    26581 caataccgcc aaacgaggcg ttgatcgccg ccgcaatgtt gttggcgtct ttaaacgacg
    26641 ggcgtttcag gttgagggtg atggtatcgc ccatctgaaa atcgctgggg atctcgcgct
    26701 ccaccgttgc gccgttagga attaacccgg cggttggcgt attaaccgtt acgctggaac
    26761 cgctggcccc ggtggcgttc atcccgccca ccaccacgct gccctgtgcc agggcgtaaa
    26821 cttccccatc agcgccgtgc agttgggtca gcagcaaagt gccgccgcga atacttttgg
    26881 catcgccaat cgacgaaacg gtgacatcaa tggtctgccc gcgcgagtac atcggcggca
    26941 gcgtggcgct caccgccacc gcggcgacgt ttttcacctt cgggtcgatt ttattcggca
    27001 gttgcacgcc aaactggcgc agcatattgg tgatggtctg gttggtgaat ttgacctggt
    27061 ttttatcacc ggtgccgtcg aggccgacca ccaggctata gcccaccagt tggttttcgc
    27121 gcacgccctg tacgttgacc agcgactcca gcgattgcgc cagcgccaca ccaggcagcg
    27181 ccaggctcac ggcgataact gcggccttta tccagttttg cattgcactc tccggcatta
    27241 aatcgggaac agaggatggt taaacagccg cgtcagccag cctgcggcgt tggcgtcgct
    27301 taacgcgccg cgtccggcat aggagatgca ggcatcggcg atccgctggg aggaaaccga
    27361 gttgtcgttc tggatgtcgt cggcgcgcac caggccgctt aagcggatgt actcatcgcc
    27421 ctggttgagg gtgagccatt tctcgccgcg aatttccagg atgccgttgg gctgcaccgc
    27481 atggacggaa acggtgatct ccccgcgcag gctgttctgc tgctgggaag tggcgctgcc
    27541 gtcaaagtcg cggttggcgt cgatagagcc ggaaagctta tctttagtgt gactaaaaat
    27601 ggtcggtgcg ccgatatcga cggtgttgct tttgccgaag ttggttttcg cctgtttgct
    27661 cgactgggtg gattcttcga ggatcaccgt caggatatcg cccacgcgat aagcccgccg
    27721 atcggcggtc agcgaccagt tgtagccagt ttcaaataca ccgccagcac gcccgtctgc
    27781 cggaggcggt gcttcggttt tcggtggcgc aaagtatgcg tcgtcttttt tgaccagaat
    27841 ggcctgcgat tcacacccgc taagcagggg aagcagcgcc accagccaga gataattttt
    27901 cacgttctgc ctgtttaaat acagtgagtt atgcgctttt gtaggtcgga taagacgcgt
    27961 cagcgtcgca tccgacattt cggccttaca gcgtctgact gatgtactgc aacatatcgt
    28021 ccgcagccga caccattttg gcgttcatct cgtaagcccg ttgcactgta atcatcgcca
    28081 ccatttcgtt gacgatatct acgttcgagc cttcgagggc attgtcctgc aaagtgccaa
    28141 gcccatcttc gcccggcaca ccttcggtcg gctgaccgct ggcggcggtt tccagataca
    28201 ggttgtcacc ctcagcggaa agcccagccg ggttagcgaa gttaaccagc gtcagttgcc
    28261 ctaactcggt cacgtcgctg tcgccaggaa gaatcgccgt caccgtgccg tcctcaccaa
    28321 acgcaacatt cgtcgccccc gccggaacgt cgatttccgg ctgcaatggc aagccctgtg
    28381 agttggtcag tacgccatcg gcgttcacct gcaaattgcc gtcgcgggta taggcgatat
    28441 cgccgttggc cttttgcacc tgcaaaaatc cctgccccat aatggcgaca ttcatcgcgt
    28501 tgtcggtggt ttcgacgttg ccttcggtga aggttttctg ggtgccgaca atgcgcacgc
    28561 cgctaccgaa ttgcagcccg gtcggcatga tgttgttctg gtcgagcatc gcgcccggcg
    28621 cttcctgggt ctggtagaac agatcctgaa acatcacccg gtcacgcttg aagccggtgg
    28681 tgttaacgtt ggcgatgttg ttggcaatcg cgctcatttc agcatcctgc gccgagaggc
    28741 cggttttgct gatccataat gctgcgttca tggtggtaaa ttccgtgtta tttttgggcc
    28801 gcggttgccg gatgcggcgt gaacgcctta tccggcctac gatgtgcggt tacgagccgc
    28861 gcaataagcg gttgcccgcg tcgctgatat cttcggcggt tttcatcatc ttgatctgtg
    28921 cttcgaactg gcggttcatg gcgatggacg acatcatttc gcttaccgcc gagacgttgc
    28981 tgccctcgag aaaaccgccg ctgactttga tgttttcatc gcgctgggcc ggaacgccgt
    29041 cggcagtcac cagcatccct tcagcatttt tctccaggtt agccaccggg atatccacca
    29101 gcttcaggcg atcgatatcc atcgtcgcgg tcacatcgcc gtcatcgggc gtcaccgaca
    29161 gcgtgccgtc gctgccaaac gaagcaatgg cgttcggcgg cagaatgatc ggcccgttat
    29221 cccccagcac cagattgccg tcgatggtca gttgcccctg gtcgtcctgc tgaatgttgc
    29281 cgttgcgggt atagacttcg ttaccgtttt tatcctgcac cgcaatgtaa cccgcgccct
    29341 gaatcgccac gtccagcggg cgttcggttt tctccgcaac acccgtgctg tcgttcacgc
    29401 cgctggcccc ttcctgcgcc atatagcgcg tgtcgaagcc gccgcctttc accttgtcgt
    29461 tggttgccat tgccatatcg gcgcgaaaac cattggtgtt gacgttcgcc aggttgttgg
    29521 cgctaatttg ctgctcatac agggtctgag aagcaccgct cagggcggta taaatcaggc
    29581 ggtccattac attgcctgga acagcgcgtc atcgagctgc gtgctggtgg cgataacttt
    29641 ggtgttggcc tgatagttac gctgggcggt catcaggttg accagttcgc tggtgatatc
    29701 gacgttagag ctttcgagca cgcccgacga cagcgtaccg caggtgccgg agccgggaac
    29761 gccaatcagc ggggtgccgg attcgcccgt ttgtacccat gcggtgccgc tcactgcctc
    29821 caggccgttt tcattcggga aagtcgccag caccacctgc ccctgcaaca tgcgctcgcc
    29881 gttgctgtag gtggcgtaaa ctttgccatc gtcatcaacc tgaacaccgt tttgcgtggc
    29941 ggaggcgtag ccgttggcgg cgttggtggt gaccgaaaat tcagagccgt attgcgtaca
    30001 ggtggagtaa tcgacggtta agtcgacggg cgcggcggcg tcggtttgaa attcaatggt
    30061 ctgcggcgtg gttggagagg tcagcttccc ggtgttcgga tcgaaggtta acgtcgtcgc
    30121 cggaacgccg gtctgctgct gaccgtcgaa ggtgtactgc acttcccagg tgttgtcgct
    30181 ggttttggtg aagtactggc ataccgaatg ttcgttgcct aatgagtcat acaccgtggt
    30241 ggtgtagctg tcggtgtacg aactgctgtt agtcgcgtcg aacggaacgg tggtgcgatc
    30301 gatagcttca tcgctggcgt tgaagttagc ggtaaaggtc aggctgctgc tggcctgcgc
    30361 cgggatattg ccggttttga tctggatatc ggtgaccgta ccggtttgca gagtgccagt
    30421 gtcatcgacc ggatagccct gcaaataagc gcccgaggcg ttgacgatat agccgttttt
    30481 gtcggtttca aacgatccgg cgcgggtata ggagatattg cctgcgctgt cacacatgac
    30541 aaaaaatccg tcgtcgttaa tcgctaaatc gagcgcattg ccggtggaga ccagcgaacc
    30601 gccacgcgaa atactttgcg cggtgcccgc cacgctgacg cccatcgcct ggcttccggc
    30661 atacatggcg gaaaactggg tggtcatcga cttatagccc accgttccgg cgttagcgat
    30721 gttgttactg atcccgtcca gctgttcgtt aacggcattc agccccgttg cggcaatttc
    30781 ataactcatt gtgtcttcct gttaattgat tcttacgcca catcactgtc ggcatcgggt
    30841 acgccaaact ggctaatcat ggtgaacggc acttcgccca cgcctgcaac gttaagcacc
    30901 ggggaactgc cgtcgagcgg gatgcgcaca tcttccactt cgccggagac ttcaatcggc
    30961 acctcttctt ccccggtatt ggtcaccacc gagacgtcgt aatcgcccgg cgggatgccg
    31021 taatccgcgg gattcaccga gaaatcaacc tggcctggtc cgacggacga actgccttct
    31081 gggatcagcg agacggtgta gtcatcgcct gcggcatcgg taaagtggag atcggcggtg
    31141 gtacaggcgt cgtccagcgt agcgcgaccg ttaatggtct ggtcgctcac ctgcaaggcg
    31201 gtggtctgca ccataatgtc gtcgcccacc aggttaccga gcgccatcac ctgaatgttg
    31261 ctcatcagca ccgcgttggt gttggcctgc acgctcatct cttccatcat cgccacctgc
    31321 gccatcgacg acagctggtc gatgtattcg gtagcgtcgg tcgggtcggt cgggtcctgg
    31381 ttttggatct cggcgacaaa cagcgtcagg aaggtatcga caggcgagct atcgctgctg
    31441 ctttgcgtgt cgctggccgc cacattattg ctttccgccg tggcggtcgg ctgcggcgat
    31501 tgcgcattca gcgccagggt attcatcagg cttctcccag tttcagcaaa ctttgctgca
    31561 tacttttcac gttgttgagc acatcgacgt tggtttcgaa atcgcgcgag gccgacatca
    31621 tgtccgccat ctgctccacc acgttgacgt cgggatacca gacgtcaccg ttggcgtcgg
    31681 cgagcggaga atgcggttca tagcgtttca ccgccccgcc ggtttgcagc acgtcctgta
    31741 cctgcacgct ggcaccgtca atggcatgac gatgtgtacc cgccagcagg ctgtggtgat
    31801 acaccgcggc aaacaccggg ctgcgcgctt tatacgcctg cgcctcgctg ctggcgggcg
    31861 cattggcatt cgccaggtta ctggcaacgg tattcagacg tagcgtttgc gccgtcatcg
    31921 ccgagccgga aatctgatag atatcagtaa atgacatgct tatttccctt caatggcctc
    31981 tctgatgccg ctgatttgca gattcagaaa ggtcaaactg ctttgataat ccatactatt
    32041 ttgtgaaaac cgcgcctgct cgctgttcag ttccacggtg ttgccatctt ccgacggctg
    32101 catcggcacg cggtactgga catccaccgc cgcgttattt gcccgttgca tctccctggc
    32161 aaaatcaata tctttagcct tgaaatttgg cgtatcgacg ttcgccagat tcgcagtcag
    32221 tagctcggtc ctctcaagac gtaacttcac tgcctgcgga tgtactccca atgcctgctg
    32281 aaaactgatc cccatttcct gcatcactcc tgcttcaatc cgcatacgct aagcaatcgc
    32341 catgccattt tttaagtcac tgatgagaaa gggtttatga gttttcaatt ttgcaggcgg
    32401 aagttttact tcctcctggc gctggcctta ccgggctacg ccgccgtcca tcccgtgcaa
    32461 cacagcgcgc gggaacaagt caacgcccag gtgctgaatg ccgcgagcca aaaaattgaa
    32521 tcgctggcgc agcagcggca gtggcatgac tatcgctaca cctttaagat ctacattccc
    32581 tcgcagattg cgaccgccgc gccctgtact aaaacacccg gcgtcacgct gacttcgcct
    32641 gcggaaatcg ccctcaaccg gatgaacttc accgttagtt gcccgcaaag ctggcagatg
    32701 aacgtggcgg tgcgcccgga tgtactggtg ccggtggtaa tgacgaagtc actggttgcc
    32761 cgcgatatgc cgctcaccgc gaatgatgtg gagcttaagc cctacaacgt cagcgcccag
    32821 cgccgcgatg tgttgatgga gcttgatgac gcgattggtt taagcagtaa gcacgcattg
    32881 caaccaggca gacccatcac caaagaagaa ctggtgtctc cggtgctggt cgggcgcgat
    32941 cagccggtga tgattgttta ccagagcgcg gggatcaccg cctcgatgcc cggcgtggcg
    33001 ctgaaaaatg gtcgtaaggg cgagatggtg aaagtgcgca atgccagcag ccagcgggtc
    33061 atcagcgcga tggtggcgga aagcggcgtg gtgacaacgg taagcgcgga gtaatttgcg
    33121 caggcgatta aagttttgcc gcctgttaac cgctttaccg cagtaaccca cccaaatacc
    33181 gagacgaggt tgcgatgaaa atcaccccga ctatgcctgg caatcgcccg acagccacaa
    33241 cggcaggctc cagccagccg cgcaaaacca cggtgaccac caccacgacg ctgagcgccg
    33301 acgacattac tcaggcggga ttgcagtctg cccagcaaac gctgaacgac cagcaagaga
    33361 gcgatatcga cagcaacaaa gtggcgcaag tgcaggcgat gctggcgtcc ggcagcccgc
    33421 aagtggacac tgaacagtta gccagcgaca tgctgagttt tttccagaac taagaggccg
    33481 atgaagtgag tatccggtta caacaggtga aggcgttgtt gcagggcatt cgtgacgatg
    33541 acgcgctgta tgattccctg cgcgaacttc tccagcgtca gcggatctgc atgattcgcc
    33601 gcgccagcga agagttgctg gcggtaaacg atgagataac ccatcactat gagcagttgc
    33661 atggacatag ccatcagcgg catagcctgc tgaaaatgct cggtgttagc gtaaatcgtg
    33721 acggtctggc gcaggttttc acctggcttc cggctgttca aaaagcggct gcacaacagt
    33781 tgtggcaacg ccttgagcaa aaagcggaac gctgcaaagc ctacaacgac aaaaacggcg
    33841 agctgttgat tcgccagtat gaatttattc agtcgttttt aggcagcgaa gcagattttc
    33901 tctatcagga ataacgcgaa taccttcccc atagcggaaa tggattttcc gcctgatttc
    33961 caatcccttg tttttaatta caaaaatccc cggcacgctt attgcgtatc ctgcacagca
    34021 ttaaagactg gaagccacct atgagtttta ttgaatgtta cgaaccggaa tacgtgcgca
    34081 aatttttagc gcgtcacccc ggttcgcctt tatacgtccg caaggtgtgg aaaaacgaga
    34141 ttcgcctgag cctgtcattg accgatattg ccagctgcga agcggtgctc aacgatgtca
    34201 gggcctttga tttacagctc acctaccgcc ctgtggaaga taattccgca gagctttccg
    34261 cgcgcgatgc gatagtaaac caggtgattt tgagtaccct cacgctccgt gatttaaccc
    34321 cggagctttc cctgtatgct gtcgggattt tgctctcctg cgccagcaaa atgccgggca
    34381 gagacggaga catcctggcc cgtttgacga cgctgccaca ggcgctggcc gatcatgcga
    34441 aaaaaggtac gctacaggca cagtacgccc agctcccgcc cgtgccacag ctggcgcgcc
    34501 agttgatgac gctgctcggc ggttgtgcgt ttgactggtc gattttgccg gaaagcccgc
    34561 gcaaagcctc gctgccgttg caggttactt tactgacgct gcatgacgcc aacagcgaag
    34621 ccctgttaca acagcagctt cagacgcagt ggcaaaccac ctggcaacag cattttgcca
    34681 ccgcgccctg gatgatgcgt aactggctga tttaccgcgt ttaccacgat atgattgggc
    34741 agactgacgg ggcggattat ttcccgctgg tgtgcgattt ttatctcctc cgcacgttaa
    34801 tcagtttatg gacgctggat ggttctccgc tgcggcagga ggatattttc gccctctttg
    34861 ccatgtttga acgctggcga gccagtgaaa atgcgctgtt ggttcgccag caaattcagt
    34921 cgttatgtgc cgccgacccg ctgctttcag cgttttcgct gctaacgtga gtcgctctgc
    34981 gcgcgctttg cccctcaccc taaccctctc cccaaagggg cgaggggacc gatcgagcac
    35041 ggtgttaaaa tcggcgctga ttttctccct ctccctgtgg gagagggtcg gggtgagggc
    35101 atcagcgcgc acgttcatca cattcacaca ttaactcacc caaacaccat gccaacacca
    35161 accatcatca aaacactcgc cacccaccgc gaaaaaccgt ttgtcagcgt cgtcacgcca
    35221 acctggaacc gcggcgcatt cctgccgtac ctgctctata tgtaccgcta tcaggattac
    35281 ccggcagacc gccgcgaact catcatcctc gacgattccc cacaaagcca tcaacacatc
    35341 atcgaccgcc tgaccaatgg cacgccggaa gcctttaaca tccgctacat ccatcacccg
    35401 gaaaaactgc cgctgggcaa aaaacgcaac atgctcaacg aactggcgcg cggcgaatac
    35461 atcctctgca tggatgatga cgactactat ccggcagata aaatctctta caccatcgct
    35521 atgatgcaaa aacaccgggc gctcatctcc ggttccgacc agatccccat ctggtacagc
    35581 catatcaacc gtatctttca aagccgcagc tttggcccac ataatatcct caacggcacc
    35641 ttttgctatc accgtaacta cctgaaaaag caccgttacg acgatgattg caatttaggc
    35701 gaagagaaaa gtttcaccga caatttcagc gttaacccgc tgcaactgcc cggcgagcgc
    35761 acgattttgt gtatctcgca tagccacaac acctttgata aagactttat tatcggggcc
    35821 agcacgccgg taaacacggc gcttacggat atcgtccgcg atccgctgct gcgcaatgct
    35881 tatctgagtc tgcataacgc cactcatcac caggcgatca accatcaggc gatcgatcag
    35941 gtggtcgtcg tgaatctgga taaacgcccg gatcgcttac aacagatccg tgaagaactg
    36001 gcattgctgc atattccacc cgagaagatc acccgccttg ccgccagtga agatgaaaac
    36061 ggtcaacggg gccgccgtca gtcgcacttg caggcgctgc gtctggcgca acagcacggc
    36121 tggcaaaact atctgttact ggaagatgac gcggttattc tcaagcagga gaaacatatt
    36181 caggtactga acacgctgct ggcgagcctg gcgaaaattc cctggcaggt gatgatcctc
    36241 ggcggcgaaa tttcgcaagg aacgatgcta aaaagtttgc ctgggctggt gcacgcgcgg
    36301 gactgccgca aagtgtgcgc ttatctggtg aacagccgct actacccgca actggcgcag
    36361 cagatgagca acgatgagca ctcgctggaa gactgctggc aaccgctgct acgcgccgac
    36421 aaatggctgg cctgttaccc gagcctttgt tatcagcgac cgggctttag cgatatccag
    36481 aaaaaaacga ctgataatat tgctcgctat ttcaataagt tacccgtaac caccaagcca
    36541 tccgcgttac ccatcgccga caccatcggc ttttttatgg aaacctcatt ccactacgcg
    36601 ctgtaccgcc caatcatcac cgccctacag gcgcaagggc tgagctgcac gctggtgatc
    36661 aacgaccgcg tgtttaaacc gtttctggat gagatgctgg aaacactgaa aaacatcgac
    36721 gatccgcagc ttaaaggtat gcgtctgtca gaaatgcaga cacatggtca gcgggtgaaa
    36781 tgcctggtca gcccctacca cacgcctgcg ctcaatggcc tggcggcggt gaacatccgc
    36841 gctatgtacg gcttagccaa agagacgtgg aaccacgccg actggaatcg tttttaccaa
    36901 agcattttgt gttatagcca ctacagtcag caggcgcttg cgcatttcgg cagcgcaaaa
    36961 gtggtcggca acccgcgctt cgacgcctgg cataacggca cgttcgatcg cgcgttgccg
    37021 gaaaacatcc aatcagatta ccgtaaacct acggtgctct atgccccaac gtttggcgca
    37081 ttaagctccc tgccccactg ggctgaaaaa ttgggccgct tgagcggtga tgtgaacctg
    37141 atttgcaaac tgcaccatgg cacctgctca cgcccggaag aagcagcatc gctggccctg
    37201 gtgcgcaggc atttaaaaca gcgcaccgac tccgcccgcc acacgctggc gctgctggca
    37261 aaagccgatt acgtgctgac cgataacagc gggtttattt tcgatgctat ccacgttgat
    37321 aagcgggtga tcctgctcga ctttccgggc atgaatgatt tgctcgacgg tgagaaaagt
    37381 tattccacgg cagagagcgc cgaccagcga atccgcgaaa tattgccggt tgctcatgat
    37441 gttgcagaac tgcgttattt gttgtcagag gcgtttgact gggggtcagt gcaggcgcgg
    37501 cttacggaga ttcgccatca ctattgcgat gcgtttatgg atggcaaggc gggagaacgg
    37561 gcggcgattg tgattgtgga ggcgctgggg taaaccgcgc aactgcctga tgcgcttctc
    37621 ttatcaggcc tacggatcga tcacgtcctg taggccggat aaggcgttta cgccgcatcc
    37681 ggcaagaaaa tacatctctg ttttagtact ttataaaagc cctctaatca cctcaataat
    37741 ccccgtcgtc gacaccgacg gcgtgcgggg gaaatagacc acctcacaaa tatacgcaaa
    37801 gctgtcaaac ttccctgccc agtcatcccc catcactaaa atatccgcac tgtacccgcg
    37861 caaatactcc gctttctgct ctagcgattc ttccagaaat acgccatcca cgcacgccag
    37921 cccggcgatg atcgccatgc ggtcatcctg atggtaaacc ggtgccctgc cctttttggc
    37981 gatgttcagg gcgtcggacg atacgccgac cagcagccgc tcgcccaatg cgcgggcgcg
    38041 ttgtaaaagg cgcagatgac cgacatgaaa cacatcgaag gtgccgaagg tgatgaccgt
    38101 tttcatggct actccgctaa tttgtgccgc agccagcact gcacggcgat gtcgtccacc
    38161 gctttttgcg ctttcgccgt ggcgctttct tgttgctcgc ggcgtttgtc gtccagcgtc
    38221 agggcgacta ctttctcttc gcacgccgcc gccgtgaggt ttttctgcat tcgcgtggct
    38281 ttgatattgg ccagcgtttt ttcctgctcc tgccaggcga ttaccttgcg cagggtgcct
    38341 ttgtagccgc tgacgttttt cagcgattcc accgacaccg ccgcacccgc agatgttttc
    38401 tcgacaaggt agcctaacgc cttgatattg ttgtcataac ccgcgcaaat ctgtttttgc
    38461 tttgccagct cgacggtttt gtctttgacc gatttatccc gcaggcgctg caactgcgtc
    38521 agggtgtcga tgatttgtcg catagttact gttctgcctt tttgacacca ggaaagagaa
    38581 tttgcaggcg gctttgcacc agttcgaggg aggcgggttc gcgcatctcc tggcgcaaaa
    38641 atcgctcgat ggccgggaac atttttaccg ctttgtcgac gctggcgtcg gcacctgcca
    38701 cataaccgcc gagcgggatc agaggtttga tctccatata ggcggcatag ttttgcttta
    38761 acgcgcgggc ggattgctga tgttccaggc tggtgacctg gttcatgcag cggctgatcg
    38821 actggccgat atcaatggcc ggatagtgcc ccgcttccgc cagtttgcgg gttaatacga
    38881 tatgcccgtc cagcaccgcg cgcgcgcagt cgacaatcgg atcttgctgg tcgtcacctt
    38941 ccgccagcac ggtgtagatg gcggtcatcg atcccgcgct ttcgctgttg cctgcgcttt
    39001 cgaccagttt ggggatcatg ccgaatgccg atggcggata acctttggtg gcgggcggtt
    39061 cgccgagcga gagggcgatt tcccgctgcg ccatcgcgta gcgggtcagg gaatccacca
    39121 gcagtaagac gtgatgcccg cggtcgcgaa accaggcggc gatggagtgg cacagttcgg
    39181 tggctttcag acgcatcagc ggcgattcat cggcaggcgc gacgacgacg atagactttg
    39241 ccagcccgtc cgcgcccagc gagtggtcga taaactcttt cacttcgcgc ccgcgttccc
    39301 caatcagccc aaccaccacg atatcggctt tggtctggcg ggtgatcatc cccagcagca
    39361 cgcttttccc gacgccgctg cccgccatta gtccgacgcg ctggcctttg ccgatggtca
    39421 gcaggccgtt gatcgctttc acgcccacgt ccagcggttc gtctaccgaa cgacgagtta
    39481 agggattaac cgagggcggc aacggcggca gaaggtcgtt gccgttcagg cggcctttgc
    39541 catccagcgg ttcgccgagg ccattcacca cgcggcctaa ccagctttcg cctatcagga
    39601 tgtcgtgggc tttttcttcc gggaagacgc gtgcgcctgc catcaggccg accggctgtt
    39661 taaacggcat taaataggtg atgtcgcgat taaagcccac ggcctgcgcg tcgatcagcg
    39721 tgtgattggc actttcgatg cggcacagct gcccggtcat tagcgggcag ccgacactct
    39781 ccagcaggat gccgttaagg cgcaccagcc gcccggcgac gcgggccagc gggatcgact
    39841 ccagcgagcg cagggcgtta tcgaacgcag aaaaatcact caccctgcga ctccggcgtg
    39901 agtgtttctt tcagcgcggt catgcattgt tcaaggcgat gctcgcagcc gatatccagt
    39961 tcggatttgt cggtgatcac ccggcattcg cccggtggca aatccggcga aggcgtcagc
    40021 ccccattcgc tgaccttttc cggcaccgcg tcgcgcaggc ggttaaactc ttccgtgctc
    40081 agcattactt gcagggtttc cggcatcgcc gggaaggccg ccagcgcctc ttccaccagc
    40141 gccagcaact gcgtgggttg cagcgccagt tcacaacgga tcacctggcg ggtgactttt
    40201 tccaccagtt gcagcaaatc ttcccgctgt ttacgttcga tatgagcgag gaagtcgttc
    40261 actttaccgg tgatggcttc cagcggctgc gccgcctcaa caaactgttt gcgcccttcc
    40321 tgctgcccgg cgaggctgcc ttcggtgtaa ccctggcgtc gcccctcggc aaaaccttgc
    40381 tgatgacctt cgctaaagcc ctgctcctgg ccttcggtca ttccttgagc aaaccctttt
    40441 tgcagccctt cctgaaatcc gtccatcaac tggcgctggt aatcggcggc gctaatactg
    40501 gtagtgagcg gttctccggt caccgcacgc tggcgcggcg ggaaacggtg caggcgatag
    40561 cgcccgcgca gtgtttcaat ggtcatggtt actccgccgt ctgttccgca aagagctgta
    40621 gctgaatttc gcccgcctct tccagttcac gggcgatgcc catgatttcg cggcggatct
    40681 gttcgatacg gctgaccggc accggcccca gacgcgcggt gatggcttcc agttgctgcg
    40741 cctgccgttt tggcattacc gcgtaaatcg aacggcgcag cagggcttcg gtgcctttca
    40801 gcgccaccgc ccagtcttcc atcggcactt cgtcaagcag acgtcgacgc acctcttcgt
    40861 tctggcggct gaggataaag aagtcgtaca tctcatcctg aagctgctcc agcacctctt
    40921 cgtcgcgctc gcgcatctgg tcgaggatca cctgctggtt gccctggaag cggttgacga
    40981 tatccgccgc ctgtttgatg cctttcactt ttgagccgtg ttccgaaagc accgacaggc
    41041 cgcgttcgat cagcctgtcc agttcatcca ccacgtcgcg gttgacgtcg ttgagtttcg
    41101 ccacccggta gagaatttcg ttttgcaccg attcgttgag atacgagagc accgctgccg
    41161 agatttccgg cgtcagaaac gcgaggaaca ccgcctgtaa ttgcagatgt tcttcggaga
    41221 tcagcatcgc cagctggcgt ggttccaccc attgcaggcg cgccatccgc gagcggatct
    41281 catcgccgta gatgccgttg atcacactgc tggcgatctc ggtgccgagg gctttgttga
    41341 gaataccctg caacatggat cgcgatgcgc cgttgatccc gctctgttcg cggaactcat
    41401 caaagaagtt attgatcact ttgcgcgcca tgctggtttt cacgccggaa agccgtgcca
    41461 tgttttcgct caggcgcccc acttcttcgc ggctgagttt ttgcatcacc gtcgccgccg
    41521 cctcttcgcc cagacacagt agcaggatcg ccgcctgttc caggtagctg ttgctcttat
    41581 ttgttgtcaa ttcgctcatt gctggtgatc cattgtctga gaacttcggc tacgcgatcg
    41641 gtgtcgctca tggccagttt ttgcaggaac tcgagtttta cttccaggcc ggaactctga
    41701 gacggcaggc tgtcatcgcc gggcagcgac ggcagatcga cgtttttgcg ctcctcttcc
    41761 ggcatggcaa tccacggttc gctgacggct tccgattctg gtgtgataat cggtgcacgt
    41821 accgccgtca gccgtttcat taccgggcgc accacgaaga acagcaacaa cagcgccagc
    41881 aggccgcagc caatcaggcg cacccaggcc agcacgctgt catctttcca cagcggaata
    41941 attggttcga ccggcaccgc ctgcggcaca aagttaagca gtgaaagcgt caggttatcc
    42001 ccacgttgcg cgtcaatacc ggcggcattg ttcagcaacg cggtaagctg cgcggtctgc
    42061 tccggcttcc agtttttcag cgccggagcg ttttgattga gcaccaccgc cacattgagg
    42121 cgtttcaccg caaagccggg atgctgaata tgctggacgc tacggtcgta agagtaatcg
    42181 cgcttgcttt cgctgtgttt ggataacgct tcaggcgagc gattttcttc cgtaccgtta
    42241 gtcatttgat tcgccgcaac cggcggacgg ttgctgagag agccgggcac acccatcgcc
    42301 acctgattgg tgtcgctgtc cagcacgctc tcttcgcggt ttattttcgg cgcgtcgccg
    42361 tagtgttcct gagtttcgtc gatggtgctg aggtcgagat ccggcatcac gctgacacgg
    42421 taattcccgc tgccgaccag cgaatccagc acgttcgcca cgctggcgtg ggttttgtcc
    42481 tgaatgtctt tgaggatctg atcgcgttta cgggtagcgg cagagaccgc ctcgcccgcg
    42541 ccgatgccgt cggtcagcag atttcccgcc tgatcaacca cgctaacttt gctggcgtgc
    42601 agccccggaa tgctgccgga aaccagatgc acaatggcgt tcacctggtc catattcagc
    42661 ttcgcgccgt agtgcagacg caccaccacc gaggcgctgt tttgcggctc atcgctgacc
    42721 acaaacgagc tttcttcgtt gagcgccaga tgcacccgcg cgctctccac cgcgtccagc
    42781 gacataatgc tctgcgccag ctccccttcg aggctgcgtt tatagcgcac gttctgcacg
    42841 aactggctgc tgccaagcac ttcgtctttg tccatcagct cgtagccgct gggcaaaatc
    42901 gcctgcacgc ctttggcggc aagggtcatg cgcgttttcg acagcgcctc ttccggcacc
    42961 agtatctgcc cgctttgcgg gtcaatgcgg taggtgagct tttcgccgtc cagcacggtg
    43021 acaatctgcg acacgggcag attttcctgg cgtccgtaga gcgagacgta gccgcgattg
    43081 ccggtccaca gcacgctaac gataatcgcc gtcgccgcca cggcaagccc cacacccgcc
    43141 atcagcgccc agcgtttgtt atctgcgaga cgcagtcgaa acgccggaaa cgccagggtt
    43201 aacttcttta tttgtgcatt catagggtta gatcgacata ctcatcagat cgttaaatcc
    43261 ggtggcgatt ttgttgcgca cctgcaccag cgcggagaaa gagagcgacg cctgctggct
    43321 ggcgatcatc gcacctgcca gatcatcgct tttgcccatg tcgatagccg tttgctgctg
    43381 ctccgccacc tgctggaact ggtcaacgtg accgagcgcg ccctgcataa tgcggtgaaa
    43441 cgaaacgtcc gggtcggtgg aactgaaggt catggcgggc agcaccggcg cctgggcgtt
    43501 ggcctgcatc cgctgcacgt cctgcatgat ctgcgcctgc atggtgctgt tgattgtggt
    43561 aatgctcatt tttaacttcc gggtcaggag aaaacttcga taccgtgctt gcgcatggag
    43621 gccagtcgat agcgcagtgc gcgcggggta atacctaaca ggtcggcgat tttgctgcgg
    43681 ttgccctgat atttacgcat caggtcagcg atatattgat actgggcgct tcgtccgtgc
    43741 tggcctaaat cgctggtttc ggcaatgtgt acggcgggct gagcaggcca ttgcagttcg
    43801 ctgtggtcgg caatgtcatt ttccggcagg cctaaggcgt ccgggtaaat cacgccgtcg
    43861 cgatttaaaa tcattccccg ctgaatggcg ttctccagct ggcgcacatt gccgggccag
    43921 cggtaattca gcatcgcacg gcgggtagat tctgaaaggg tgatattttt caccagcacc
    43981 gtggagtatt tttttataaa tgactctgcc agcggaataa tatcgttcag acgttcacgc
    44041 agcggcggca tggtaatagg aataaccgcc agacgataat agagatcttc acgaaaacgc
    44101 ccggcggcga cttcctgctc aaggttttta ttggtgcagg caatcaggcg aaaattgagt
    44161 ttaatttgtc ggttgctgcc taatcgctca acctgttgtt cctgcaatac gcgtaatatt
    44221 ttggcctgta acgccagcgg catatcgcca atttcatcga gtaataaggt gccgttattc
    44281 gccagttcca tttttcctgg cacactggca attgcgccgg taaatgcgcc tttgtcgtag
    44341 ccaaataagg ttgcttccag catgttttcc gggatcgcag cgcagttcac gccgatatag
    44401 ggcgcgtttt cattttcacc aaaggcgact gtgtgaatat atttcgccac gcactctttg
    44461 cccgcgccgg tttcgccctg aatgagcacc ggcacgttaa atgcggcgac acgctttgcc
    44521 agagtaaaag cgttaatgct ggaagcggca gttgcaataa gttcagacat agggtgaacg
    44581 atataagtag cgacaataat caggatgttc tgaagcgtat caccggcggg attaacaagt
    44641 tcgaattaaa cgataataaa aacattattt gtttataaac aatgatttat ccatgtaggc
    44701 cggataaggc gtttacgccg catccggcaa ttgtgcaccg gtgcctgatg cgatgcggcg
    44761 cgtcttatca ggcctacggt tcgtgcaatg tgttatgacg atgcctgata tctcgccctt
    44821 taataattta attatttttt caccctatta ataatttgcc cctttcacgc cccctctatt
    44881 taatacctca ccttgtgtca ctgccggatt tgcgtgcaca aaatcgccca taccattaca
    44941 gcagagaaag taagccatgc tgaagtacag taaaacgcca ggcatcttta aattagaagg
    45001 taatcggctc ggacgcccct accatcgcct gccgaccctg tttacaggga attttgacgt
    45061 gattgattcg catctgggca gctactttct caaaaagcat cgcagcaata tcaccctaaa
    45121 aaagattggc tgcgaaatgg atattattaa taaaaacgct gaactgatgg tttcccaggt
    45181 cgggcatctg gcgtttgata ttgatcgctc tttgctgtta atgctgctgg gcaattttta
    45241 tggtctggag tcctctcttg aagaggcgaa agcgcataac gggctgccga ccaaaaccga
    45301 aacgcggtta aaaaaccgtc tggcgctgga tatctgcacg ctgattttta atctgcaaac
    45361 ctccggtatt gcgctgaaat taaagctgga cagcagcacg gtaattaccc actgggcgta
    45421 tcaattaacc tttacccttg cgggcgatga agagagctgt tttcgtattt tgctcgacga
    45481 cgcccacacc gattttattt taaatctgat ccgccacagc gagcacagta aaccgcagca
    45541 ggtggcgaaa tcggtcaata aaccggccct gattaaagag atcatccgct ccctgccgct
    45601 gaccctgaac gtcaaaatcg ccgagctatc gatgaatgtc gcggatctca cgcagataaa
    45661 agccggggac attttaccca tctcgctggg agaaacgttc ccggtggcga ttgggcaatc
    45721 tgaattattt agcgccctga ttgtggaaga caaagacaaa ctgtttttgt cggagctggc
    45781 gggcaaaaat gagaatagcc atgagtaagc aagaagatat tttagccggg gattttggcc
    45841 tcaccgatga agttgcgcct gtcgcaaaat ccgccgacgc tgaaacgctg gtcacccgcc
    45901 tggaagaccg tttttccgac tcgatgacgc tgctaaaacg cattccggtg accttaacgc
    45961 tggaagtgtc atcagtagaa ataatgttag ccgacctcct taacatcgac gacgacacgg
    46021 tgattgagct ggataaactc gccggggagc cgctggatat caaagtgaac aatatcctgc
    46081 tgggcaaagc ggaagtggtg gtggtcaacg agaagtacgg cctgcgcgtg ctggaattta
    46141 acacccgcga tatcaacgac ctggcaccat gaaacgccgc actcaactga cgctgggcct
    46201 tgggctgctg gcgcttgcgc cggtggcgtt ggcccagggc ggcgatattg cgctgcttaa
    46261 cgtggtcacc cacggcaaca ctcaggagta cagcgtcaaa atccaggtgc tgattctgat
    46321 gaccctcgtc ggtttgctgc ctacgatggt gctgatgatg acctgcttta cgcgctttat
    46381 tatcgtgctg tcgctgctgc gccaggcgct agggctacaa caaacgccgc caaaccgcat
    46441 tctgattggc attgcgctct ccctcaccat gctggtgatg cgccccatct ggctcaatat
    46501 ttacgaccac gcagtagtgc cgtttgaaaa tgatcagatt accctgaccg atgcgttaag
    46561 caccgccgcg acgccactca aacgctttat gctggcgcag actgacaaaa aagcgatggc
    46621 gcaaatcatg actatcggcg gcgcgaaggg ccatgccgcc gaccaggatc tctcgattgt
    46681 ggtgcccgcc tatgtgttga gcgagctgaa aaccgccttc cagattggct ttatgattta
    46741 catcccgttt ctggttatcg acctgattgt cgccagcgta ctgatggcga tggggatgat
    46801 gatgctgtcg ccgctgatcg tttcgctacc ctttaagctg atgctgtttg tgctgatcga
    46861 cggctggtcg ctgaccatcg gtacactcac caccagcatt cgcgggctgg ggctgggctg
    46921 atgatgctga ccatagacgt agccgccgac attgtcgcca gcggcataaa agtggtgatt
    46981 ttgctggttt cggtgctggt agtgccgagc ctgctggtcg gcctgctggt gagcgtgttc
    47041 caggcggtaa cgcagattaa cgaacagacc ctaagctttt tgccgaggct gattgtcacg
    47101 ctggtggtgc tgggcgtgtg cggtaaatgg atgattatcc agttgcatga tctctgcatt
    47161 cacctgttta cccaggccgc gttgctggtt cattgagatg cgcacaagcg acgttacgca
    47221 actgatggat ctggcgctgg ggctgtggtt tccctttgtg cgcattatgg cgtttctgcg
    47281 ctatgtgcct gtgctggata acagcgccct gaccgtgcgc gtgcggatta ttttgtcgct
    47341 ggcgctggca atcatcatca cgccgttgat cccccatcct gttccccacg acctgatatc
    47401 gctcaacagc ctgatcctga cggtggagca aattttatgg ggcatgttat tcgggctgat
    47461 gtttcagttt ttgtttctgg cgctacagct ggccgggcaa attctctcgt tcaatatggg
    47521 gatgagcatg gcggtgatga acgacccgag cagtggcgca tcgaccaccg tgctggcgga
    47581 gctcatcaac gtctacgcga tcctgctgtt ttttgccatg gacgggcatc tgctgctggt
    47641 gagtgtgctg tataagggct ttacctactg gccgattggc aacgcgctgc atccgcaaac
    47701 cctgcgcacc atcgcgctgg cctttagctg ggtgctggcg tccgcatcgc tgctggcgct
    47761 gccaaccact tttatcatgc tgattgtgca gggctgcttt ggcctgctca accgcattgc
    47821 gccgccgctg aacctttttt cactcggttt tcccatcaac atgctggcgg ggctggtctg
    47881 ctttgccacc ctgctctaca acctgccgga tcactatctg catctggcga attttgtgtt
    47941 gcagcaactc gacgcgctga aaggtcacta tggcggatag cagcagcgaa gaaaaaacag
    48001 agaaaccctc ggcgcaaaag ctgcgtaaag cgcgcgaaga ggggcaactg ccgcgctcga
    48061 aggatatggg gctggcggcg agcctgtttg cggcatttgt ggtgatctcc agcagcttcc
    48121 cgtggtatgc cgattttgtg cgcgagagtt ttatcagcgt gcaccagtat gcacaggaga
    48181 ttaataaccc agaggtgatc ggccagtttc tgcgccatca cctgttaatt ctcggcaagt
    48241 ttattctcac cttgctgccg atgcccgccg ccgcgctact ctcctcacta gtgcccggcg
    48301 gctggctgtt tctgcctaaa aaaatcctcc cggatttcag caaaatcagc ccgttaaaag
    48361 gcattgagcg gctgttttcc agtgaacatc tggcggaaac gggaaagatg acggtgaagt
    48421 cggtggtggt gctggtgatg ctgtgggtaa gcctgcgcaa taactttgcc tcctttctcg
    48481 gcctgcaggc gctaccgttc aaactggcga tgaatgaggg tttatcgctc tacgcctgcg
    48541 tgatgcgcaa ctttgtcatc ctgtttatct tctttgcgtt aatcgacgtg ccgctggcga
    48601 aagccctgtt taccaaaggg ctgaagatga ccaaacagga gctgaaagaa gagtataaaa
    48661 accaggaagg caagccggaa gtgaaggcgc gcgtgcggcg tctgcaacgg cagttggcga
    48721 tggggcaaat tcgcaaagtg gtgccgaaag ccaacgtggt gatcaccaac ccgacccact
    48781 acgccgtggc gttgcagtat gactcgtcgc gcgccgccgc gccgtttgtg gtggcaaaag
    48841 gcaccgatga aatcgccctc tatattcgcc aggtcgccgc tgagaatcag gtggaagtgg
    48901 tcgagttccc gcgactggcg cgctcggttt actacaccac ccaggtgaac cagcaaattc
    48961 cgtttcagct ttaccgggcc atcgcccacg ttctgaccta cgtcctgcaa atgaaacact
    49021 ggcgcgaggg tgcacagccg cgcccggccc tgaacagaca tatttccatt cccaaagagg
    49081 tgcttaaact ggatggcgaa aacaactaaa tcgttcctcg cgctgctgcg cggcggcaat
    49141 cttggcgtgc cgctggtgat cctgtgtatt ctggcgatgg ttattttgcc gctgccgcct
    49201 gcgctgctgg atattctgtt caccttcaac attgtgctgg cggtgatggt gctgctggtg
    49261 gcggtgtcgg cgaaacgacc gctggaattc agcctgttcc cgaccatttt gctgatcacc
    49321 accttaatgc gcctgacgct caacgtcgcc tctacgcgcg tggtgctgct gcacgggcat
    49381 ctcggcgcgg acgcggcggg taaggtgatt gagtcgtttg gtcaggtggt gatcggcggc
    49441 aactttgttg tcggctttgt ggtgtttatc atcctgatga tcatcaactt tattgtcgtc
    49501 accaaagggg ccgagcgtat ttccgaggtt tcagcccgct ttaccctgga tgcgatgccc
    49561 ggtaagcaga tggcgattga cgccgatctt aatgccgggt tgatcaatca ggcgcaggca
    49621 caaacccgac gtaaagatgt tgccagcgag gccgatttct acggcgcgat ggacggggca
    49681 tcgaaatttg tgcgcgggga tgctatcgcc gggatgatga ttctggcgat caacctgatc
    49741 ggcggcgtct gtatcgggat cttcaaatac aacctgagcg ccgacgccgc cttccagcag
    49801 tatgtgctga tgaccatcgg cgacgggctg gtggcgcaaa tcccttccct gctactctcc
    49861 accgcggcgg cgattatcgt cacccgcgtc agcgacaacg gcgatatcgc ccatgacgtg
    49921 cgtcaccagt tgctggcaag cccatcggtg ctctacaccg ctaccggaat tatgttcgta
    49981 ctggcggtag tgcctggaat gccacatctg ccgtttttgc tgttcagcgc cctgcttggc
    50041 tttaccggct ggcggatgag caaacgcccg caggcggcgg aagcggaaga gaaaagtctc
    50101 gaaacgctga cccgcaccat caccgaaacc agcgagcaac aggtcagttg ggaaaccatt
    50161 ccgctgatcg agcccatcag cttaagcctc ggctacaagc tggtggcgct ggtggacaaa
    50221 gcccagggca acccgctcac ccagcgtatt cgcggcgtgc gacaggtgat atccgacggc
    50281 aacggcgtgc tgctgccgga gatccgcatt cgggaaaact tccgccttaa gcccagccag
    50341 tacgctattt tcatcaacgg cattaaggct gatgaagcgg atattccggc ggataaactg
    50401 atggcgctgc cctccagcga aacctacggc gagatagacg gcgtgctggg gaacgacccg
    50461 gcgtacggga tgccggtgac ctggatccag cctgcgcaga aagcgaaggc gctgaatatg
    50521 gggtatcagg tgatcgacag cgccagcgtg attgctaccc atgtaaataa gattgtgcgc
    50581 agctatattc ctgatttatt taactatgac gatattacgc agttgcataa ccgtttggcg
    50641 tcgatggcac cgcgcctggc ggaggattta agcgcggtgc tcaattacag ccagttgctg
    50701 aaagtgtacc gggcgctgct gaccgaaggc gtttccctgc gcgatatcgt caccatcgcc
    50761 accgtgctgg tcgccagtag cgcggtgacc aaagatcata ttctgctggc agccgatgtg
    50821 cgcctggcgc tgcggcgcag cattacccat ccgttcgttc gcaagcagga gctgacggtg
    50881 tataccctga ataatgagct ggaaaatctg ctgactaacc tagtgaatca ggcgcaacag
    50941 ggcggtaaag tgatgctcga cagcgtgcca gtggacccga atatgctcaa ccagttccag
    51001 agtacgatgc cacaggtgaa agagcagatg aaagcggcgg ggaaagaccc ggtgctgctg
    51061 gtgccaccgc agctgcgccc tttgctggcg cgttatgcaa ggttgtttgc gccggggctg
    51121 catgtgctgt cgtataacga agtgccggat gagctggagt tgaagattat gggggcgttg
    51181 agttgatggg ggattctttt ccatgctttg cagcacctga caccattaat tgacgtcaca
    51241 ctgcaaagag actacgatga caccatacaa tgacgtcatg gattgatagt atgattaagg
    51301 tcagcgcgct aggttctcgt catagaaaaa cattcgacga tgtaatgacg gagccgccaa
    51361 aatctggaat taaatgggat gatgttgttg cactcattaa agctgtgggt ggaacaataa
    51421 aaaataacaa cggctccaga cgaaaatttc agattggaac gaccaaattc agcacacatg
    51481 agccacatcc aaaaaatgtg atggataaag gcgcagtagc aggtcttaag gagtggtttg
    51541 ttaactgtgt aggggtagat tatgaataaa gcatcgaaca ttatcacaat taaaggtcaa
    51601 ccagccgcag taacatttga agccgatatc aacgcatttc gcggcaagtt cctcaatgta
    51661 aacggttatt gtgactttgt agccacaagt attgaggcat tatatcgtga aggcgaaagc
    51721 gcgctggatg agtggatggc ggattgtgaa gaggacggta ttgccccttt tcgtgaagaa
    51781 gaagagcaaa aacgcctgac gcttcgtgtt cctcatcaca ttgattcgcg gctgaccatc
    51841 gtcgccagac aacattccat ttcaaaaaac cagctcattg ttgatgtctt agaacgtgag
    51901 ttttcagccc agatgtaaac aggtcggatg ccgcgtggca tccgaccttt tgccagttcg
    51961 tcaatattac ccatttacaa aatcgtctcc tccgtcaaaa tccgccgcgc gcctaaaaag
    52021 tgatcctgcc agaaaggttc agctaactga cttacccgaa tggtttcccc ggtgcgcggt
    52081 gactcaataa actgaccgtc gcccagatac acccccatat ggtcggcaat ctcgcggctg
    52141 tggatatgga aaaatagcaa atctccccgg cgcaggtcgt tattcgccac aatggttgcc
    52201 cggcgatagt gatacatctc attagccgtg cgtgggagtt tcgcctcaag gatcttgttg
    52261 taggcataaa aaactaaccc gctacagtcg aagccttgat cgggccgcgt accgccccag
    52321 acgtaaggct tgccaagctg ttgctccaga cggtgaatcg ccacttcggt gatgttatgc
    52381 agatggtcgc tgcttaagaa atggttgttt tccgccagcg cctgccagcg tctgtcactc
    52441 gccttgagcg gcgcaggaaa ccattctgga tgctgtttta tctgttcgcg gttgtgctgg
    52501 cgtagcgccc ttctgctttc ggcattgcct tccacaatat agctggcctg ttttttcaga
    52561 tgggtttggt actgttttaa taaacgcgca cgattttgca tccgctggcg ggcggcgtag
    52621 ctgaaagtga tatgtgatgt ggtggcgctg gcaaatgaag aaaacggcaa gcaaagcaac
    52681 agtaatcccg acaacacaaa agagggaatc gacggcaagg acatactcaa taatggctga
    52741 tcatgcattg atgttgcagt gtatcccggc ccgtacctga acaaacttaa taaaccatta
    52801 ttataaaatt aataaatata tttcagttaa ttatgtaaaa acatccgttt attaaattac
    52861 tcatggattg tcgctgttgc tcatttgagc tacaattcaa gctgaataaa tatacagcac
    52921 aggagatacc ccaatggctg ctaacgcatt tgttcgcgcc cgaatcgatg aagatctgaa
    52981 gaatcaggcg gcggacgtac tggccgggat ggggctgacc atctctgacc tggttcgcat
    53041 aaccctcaca aaggtcgcgc gtgaaaaggc attgccgttt gatttacgcg agcctaatca
    53101 attaaccatt caatcaatca aaaacagcga agctggcgtt gatgttcata aggccaaaga
    53161 cgccgatgat ttatttgata aattaggaat ttaaatcaaa gggatattga atactcggga
    53221 caattttcaa aggatgtaaa acttgcacaa aagcgtcata aggatatgaa taaattgaaa
    53281 tatcttatga cgcttcttat caataatgct ttaccgcttc cagctgttta taaagaccac
    53341 ccgctgcaag gttcatggaa aggttatcgc gatgctcatg tcgaaccgga ctggatcctg
    53401 atttacaaac ttaccgataa acttttacga tttgagagaa ctggaactca cgcggcgctc
    53461 tttgggtaaa gcacgctgcc gatgaaattt gtaaaccctg cgccgacagg cttgcgtaaa
    53521 actcttttgg tgataatgtg agccttccaa atccagacta ttccgatttg ggatctattt
    53581 tgccgtcctg gcgtgtgtaa ccgttttatc aaggaataag cagtatgcgt aaaatcgcat
    53641 taattcttgc gatgttgttg atcccgtgcg tttcgtttgc cggtttgctg ggcagcagta
    53701 gctcgaccac gccagtgagc aaagagtata agcagcagtt gatgggatcc cctgtctaca
    53761 tccagatctt caaggaagaa cgtacgctcg atctctacgt caaaatgggc gagcaatatc
    53821 agctgctcga cagttataaa atctgtaaat attccggcgg cttagggcca aaacagcgtc
    53881 agggcgattt caaaagcccg gaagggtttt atagcgtcca gcgtaatcag ttaaaaccag
    53941 acagccgtta ctacaaagcg attaatattg gtttccccaa tgcctatgac cgtgcgcatg
    54001 gttatgaagg gaaatacctg atgatccacg gcgattgtgt ttccatcggc tgctacgcaa
    54061 tgaccaatca gggtattgat gagatcttcc agttcgttac tggtgcgctg gtgtttggtc
    54121 agccgagcgt gcaagtgagt atttacccgt tccgcatgac cgacgccaat atgaagcgcc
    54181 ataaatattc caactttaag gacttctggg agcagctgaa gccgggctac gactactttg
    54241 agcaaacccg taagccaccg accgtttctg tggtcaatgg ccgttacgtg gtcagcaagc
    54301 cgttaagcca cgaagttgtg cagccacaac tggcatcaaa ctacacgctc cccgaggcaa
    54361 aataagcgcc attcgcctgg cataatcttt tgccaggttt cattgcccgt cagcggctgt
    54421 gttgcaatca ccgtgaccac atcattcggt gtggtctgcg agctgaagtc gatttccaca
    54481 tcctgatcca gcagcgttgc cacgccaaac ggcgcgcggc gggtgatcca gtgtaaatta
    54541 gtcgagcaat acgccatcac atagcgcccg tcagaaagca gcatgttgaa aacgcccttc
    54601 tgccgcagtt catccgccag tgaggcgata tatttaaaca ctgccgccat gttgcccggt
    54661 gtgcgcgggt aacgctgcgt taatttatgc aggagccagc agaaggcttt ttcgctatcg
    54721 gtttcaccga ccgggcggaa gttgccggtt tccagtgatt tgtagcccgt cagttgtccg
    54781 ttatgggcat aagtccagtt acgcccccat aactcgcggg taaatgggtg agtattttcc
    54841 agcgccacct cgccccgatt agcctggcga atatgagcca ccaccgaaca ggattttatc
    54901 gggtagtcct ggacaagttt ggcgatgggg gaattaaagc tgggttgtgg atctttaaat
    54961 gtgcgacagc ctttaccttc gtaaaaggta atgccccagc catctttatg tggcccggtt
    55021 ccaccaccac gctgtacaag tccggtgaaa ctaaagcaga tatcggtagg gacgttggcg
    55081 ctcatcccga gcaattcgca catgggtaac ctccacaaca gcgggagcga atggcccccg
    55141 gcgaagattg atttaagaat tttcaggtcg gatgcggcgt aaacgaatta ttcggcctac
    55201 ggttcgggta cagatttgta ggcatgataa gacgcgtcag catcgcatca ggcatcagcg
    55261 cacaagtgcc ggatgcggcg taaacgcctt atccagccta cgccagactt acttaaccat
    55321 ctctttttca attaactgga tcaggatatg gatcacttta atgtgaatct cctgaatgcg
    55381 gtcggcataa ccaaagtgcg gtacgcgaat ttcgatatcc gccgtgccag ccattttgcc
    55441 gccgtcttta ccggtcaggg tgatcacttt catccccttc tcacgcgccg ctgcgatcgc
    55501 tttgatcacg tttgcagagt taccggaggt ggagatcccc agcagtacat cgccttcgcg
    55561 acctaccgct tcaacgtagc gggagaaaat atcattgaaa ccgaaatcat taccgacgca
    55621 ggaaatatga ctaacgtcag aaatagcaat cgccgggtag cccggacggt tttcacggta
    55681 gcgaccggtc aactcttcgg caaagtgcat agcgtcgcaa tgggaaccgc cgttgccgca
    55741 ggaaagcact ttgccaccgg ctttaaagct gtctgctaac aggaccgccg cgcgctgaat
    55801 ggcgtgaata ttggcgtcat cttttaaaaa gttagccagc gtttccgccg cttcgttcag
    55861 ttcgttacga ataagatcct ggtacatgag gatatccttc agcataaatg taatagacaa
    55921 aatgcagtgt accggatacc gccaaaagcg agaagtacgg gcaggtgcta tgaccaggac
    55981 tttttgacct gaagtgcgga taaaaacagc aacaatgtga gctttgttgt aattatattg
    56041 taaacacatt gctaaatgtt tttacatcca ctacaaccat atcatcacaa gtggtcagac
    56101 ctcctacaag taaggggctt ttcgttatga tgattttgag tattctcgct acggttgtcc
    56161 tgctcggcgc gttgttctat caccgcgtga gcttatttat cagcagtctg attttgctcg
    56221 cctggacagc ctccctcggc gttgctggtc tgtggtcggc gtgggtactg gtgcctctgg
    56281 ccattatcct cgtgccattt aactttgcgc ctatgcgtaa gtcgatgatt tccgcgccgg
    56341 tatttcgcgg tttccgtaag gtgatgccgc cgatgtcgcg cactgagaaa gaagcgattg
    56401 atgcgggcac cacctggtgg gagggcgact tgttccaggg caagccggac tggaaaaagc
    56461 tgcataacta tccgcagccg cgcctgaccg ccgaagagca agcgtttctc gacggcccgg
    56521 tagaagaagc ctgccggatg gcgaatgatt tccagatcac ccatgagctg gcggatctgc
    56581 cgccggagtt gtgggcgtac cttaaagagc atcgtttctt cgcgatgatc atcaaaaaag
    56641 agtacggcgg gctggagttc tcggcttatg cccagtctcg cgtgctgcaa aaactctccg
    56701 gcgtgagcgg gatcctggcg attaccgtcg gcgtgccaaa ctcattaggc ccgggcgaac
    56761 tgttgcaaca ttacggcact gacgagcaga aagatcacta tctgccgcgt ctggcgcgtg
    56821 gtcaggagat cccctgcttt gcactgacca gcccggaagc gggttccgat gcgggcgcga
    56881 ttccggacac cgggattgtc tgcatgggcg aatggcaggg ccagcaggtg ctggggatgc
    56941 gtctgacctg gaacaaacgc tacattacgc tggcaccgat tgcgactgtg cttgggctgg
    57001 cgtttaaact ctccgacccg gaaaagctgc tgggtggcgc agaagattta ggtattacct
    57061 gtgcgctgat cccgaccacc acgccgggcg tggaaattgg tcgtcgccac ttcccgctga
    57121 acgtaccgtt ccagaacgga ccgacgcgcg gtaaagatgt cttcgtaccg atcgattaca
    57181 tcatcggtgg gccgaaaatg gccgggcaag gctggcggat gctggtggag tgcctctcgg
    57241 taggccgcgg catcaccctg ccttccaact caaccggcgg cgtgaaatcg gtagcgctgg
    57301 caacgggcgc gtatgctcac attcgccgtc agttcaaaat ctctatcggt aagatggaag
    57361 ggattgaaga gccgctggcg cgtattgccg gtaatgccta cgtgatggat gctgcggcat
    57421 cgctgattac ctacggcatt atgctcggcg aaaaacctgc cgtgctgtcg gctatcgtta
    57481 agtatcactg tacccaccgc gggcagcagt cgattattga tgcgatggat atcactggtg
    57541 gcaaaggcat tatgctcggg cagagcaact tcctggcgcg tgcttaccag ggcgcaccga
    57601 ttgccatcac cgttgaaggg gccaacattc tgacccgcag catgatgatc ttcggacaag
    57661 gagcgattcg ttgccatccg tacgtgctgg aagagatgga agcagcgaag aacaatgacg
    57721 tcaacgcgtt cgataaactg ttgttcaaac atatcggtca cgtcggtagc aacaaagttc
    57781 gcagcttctg gctgggcctg acgcgcggtt taaccagcag cacgccaacc ggcgatgcca
    57841 ctaaacgcta ctatcagcac ctgaaccgcc tgagcgccaa cctcgccctg ctttctgatg
    57901 tctcgatggc tgtgctgggc ggcagcctga aacgtcgtga gcgcatctcg gcacgtctgg
    57961 gggatatttt aagccagctt tacctcgcct ctgccgttct gaagcgttat gacgacgaag
    58021 gccgtaatga agccgacctg ccgctggtgc actggggcgt acaagatgcg ctgtatcagg
    58081 ctgaacaggc gatggatgat ttactgcaaa acttcccgaa ccgcgtggtt gccgggctgc
    58141 tgaatgtggt gatcttcccg accggacgtc attatctggc accttctgac aagctggatc
    58201 ataaagtggc gaagatttta caagtgccga acgccacccg ttcccgcatt ggtcgcggtc
    58261 agtacctgac gccgagcgag cataatccgg ttggcttgct ggaagaggcg ctggtggatg
    58321 tgattgccgc cgacccaatt catcagcgga tctgtaaaga gctgggtaaa aacctgccgt
    58381 ttacccgtct ggatgaactg gcgcacaacg cgctggcgaa ggggctgatt gataaagatg
    58441 aagccgctat tctggtgaaa gctgaagaaa gccgtctgcg cagtattaac gttgatgact
    58501 ttgatccgga agagctggcg acgaagccgg taaagttgcc ggagaaagtg cggaaagttg
    58561 aagccgcgta attgaataac ggagccgaaa ggctccgttt ctttatccgc taattattta
    58621 aaattaaagc catccggatg gttttccagg ctgccggtca acgccgcgaa cagcaccgtt
    58681 ttaccatcaa tcgaaagcgc atcgttcaca ttcagccagg tgagtttctc ttgcgacgtt
    58741 ttctcatcaa tagtcgagaa cagccccgtc atctgattag atttctcgga ccacatcaca
    58801 gcgatacgtt gcgagccaca gtcatgcggt ttgcacgcgc tcatcacctg atacgtctca
    58861 tctcccaacg ttacggtttg tgcgggagta taagtaccgc ctttcatcac ccaggcaggc
    58921 agcttatgcc cttgtaccat ctgattaaat gcagctttgg tggtttcgcc ctttgcaagg
    58981 ctgctaatgg ttaaatcatc ctgcgccatt gcactggtgg cgatgaccag agcggcgact
    59041 gtcgttattg ccttaaacat cattcctccc gagcttatcc tgcccatgtt attaaccttc
    59101 taaagcctgg cgataacacg ataatttgca aatcagatat ttccaatatc agcatttgtt
    59161 atcaatttat tctgcgctaa ggcaactgcc tgataaggtg ttaaagttat aaaacccttc
    59221 gcataaagga gttgtacacc gtgcctggtt tgaagattac gcttttgcag caaccactgg
    59281 tgtggatgga tggccctgcc aacctgcgtc attttgatcg tcaactggaa ggtattaccg
    59341 ggcgcgatgt gatcgttcta ccggagatgt ttaccagcgg ctttgccatg gaagcggcag
    59401 cttcgtcgct agcacaagat gacgtagtga actggatgac agctaaggcg cagcagtgca
    59461 atgcgctgat tgcaggcagt gttgcattac aaacggagtc tggttcggtt aaccgctttt
    59521 tgctggttga gccgggcggc acggtacatt tttatgataa gcgtcatctg ttccgcatgg
    59581 cagatgagca tctacattat aaagcgggca atgcgcgagt gattgtggaa tggcgcggct
    59641 ggcgtatttt gccgctggtg tgctacgact tacgttttcc tgtgtggtcg cgcaatctca
    59701 acgattatga cctcgccctg tacgtcgcca actggcctgc tccgcgctct ctgcactggc
    59761 aggcattgct gacggcccgc gcgattgaga atcaggcgta tgtggcggga tgcaatcgcg
    59821 tcggcagcga tggcaacggc tgccattatc gcggtgacag ccgggtgatt aatccacaag
    59881 gcgagattat cgctactgcc gacgcgcatc aggcaacgcg cattgatgcc gagctgtcga
    59941 tggcggtgtt aagggaatat cgcgataagt ttccggcatg gcgggatgcg gatgagttta
    60001 ggttgtagtg agtgggggct gtatttaggg cggggaattc agaaagggat gtttagtgtg
    60061 ctgagcgaga gttaaataat aaaaaactgg gaagatttaa taatcccccc gcagggcaag
    60121 agcatgaatt aaatttgtgt taaatttttc attttatcag ttcgaaaccc aagctctgag
    60181 caggatgcaa gcacttcttc tatataacat gtgtttaacg ccagggcaag attacgaaag
    60241 cccgctcccc gcaaggactg acgccagata gtttctgtcc atggctgctt ttcgcatctt
    60301 acgtcttaac cctgccttga ataccttatc attcgtcaaa atattaatag caatgtgccg
    60361 tatccctgaa aataattctg ctgcatttcc tcttcttatt ttgcagtcgt cttcattcat
    60421 taccacgtcc agacgccagt gcagcttatt ctccacgtgc cagtgatttc ggatcgctgt
    60481 ggcgaatttc tc
//
LOCUS       DAZGOB010000011        59296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGOB010000011 DAZGOB010000000
VERSION     DAZGOB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.011"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(45..995)
                     /gene="dusC"
                     /locus_tag="ACHLIF_000730"
     CDS             complement(45..995)
                     /gene="dusC"
                     /locus_tag="ACHLIF_000730"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO9604793.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            1234..1632
                     /gene="yohJ"
                     /locus_tag="ACHLIF_000731"
     CDS             1234..1632
                     /gene="yohJ"
                     /locus_tag="ACHLIF_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO9604794.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1629..2324
                     /gene="yohK"
                     /locus_tag="ACHLIF_000732"
     CDS             1629..2324
                     /gene="yohK"
                     /locus_tag="ACHLIF_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO9604795.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2454..3338
                     /gene="cdd"
                     /locus_tag="ACHLIF_000733"
     CDS             2454..3338
                     /gene="cdd"
                     /locus_tag="ACHLIF_000733"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO9604796.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3488..4207
                     /gene="sanA"
                     /locus_tag="ACHLIF_000734"
     CDS             3488..4207
                     /gene="sanA"
                     /locus_tag="ACHLIF_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO9604797.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4210..4449
                     /gene="yeiS"
                     /locus_tag="ACHLIF_000735"
     CDS             4210..4449
                     /gene="yeiS"
                     /locus_tag="ACHLIF_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO9604798.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4768..6006
                     /gene="preT"
                     /locus_tag="ACHLIF_000736"
     CDS             4768..6006
                     /gene="preT"
                     /locus_tag="ACHLIF_000736"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO9604799.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            6000..7235
                     /gene="preA"
                     /locus_tag="ACHLIF_000737"
     CDS             6000..7235
                     /gene="preA"
                     /locus_tag="ACHLIF_000737"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO9604800.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7306..8316)
                     /gene="mglC"
                     /locus_tag="ACHLIF_000738"
     CDS             complement(7306..8316)
                     /gene="mglC"
                     /locus_tag="ACHLIF_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO9604801.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8332..9852)
                     /gene="mglA"
                     /locus_tag="ACHLIF_000739"
     CDS             complement(8332..9852)
                     /gene="mglA"
                     /locus_tag="ACHLIF_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO9604802.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDISFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(9913..10911)
                     /gene="mglB"
                     /locus_tag="ACHLIF_000740"
     CDS             complement(9913..10911)
                     /gene="mglB"
                     /locus_tag="ACHLIF_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO9604803.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11191..12231)
                     /gene="galS"
                     /locus_tag="ACHLIF_000741"
     CDS             complement(11191..12231)
                     /gene="galS"
                     /locus_tag="ACHLIF_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO9604804.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(12373..13530)
                     /gene="yeiB"
                     /locus_tag="ACHLIF_000742"
     CDS             complement(12373..13530)
                     /gene="yeiB"
                     /locus_tag="ACHLIF_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO9604805.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13547..14215)
                     /gene="folE"
                     /locus_tag="ACHLIF_000743"
     CDS             complement(13547..14215)
                     /gene="folE"
                     /locus_tag="ACHLIF_000743"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO9604806.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14473..15309
                     /gene="yeiG"
                     /locus_tag="ACHLIF_000744"
     CDS             14473..15309
                     /gene="yeiG"
                     /locus_tag="ACHLIF_000744"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO9604807.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15341..17332)
                     /gene="cirA"
                     /locus_tag="ACHLIF_000745"
     CDS             complement(15341..17332)
                     /gene="cirA"
                     /locus_tag="ACHLIF_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO9604808.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17625..19094)
                     /gene="lysP"
                     /locus_tag="ACHLIF_000746"
     CDS             complement(17625..19094)
                     /gene="lysP"
                     /locus_tag="ACHLIF_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO9604809.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19299..20180)
                     /gene="yieE"
                     /locus_tag="ACHLIF_000747"
     CDS             complement(19299..20180)
                     /gene="yieE"
                     /locus_tag="ACHLIF_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO9604810.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20279..21328
                     /gene="yeiH"
                     /locus_tag="ACHLIF_000748"
     CDS             20279..21328
                     /gene="yeiH"
                     /locus_tag="ACHLIF_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO9604811.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21402..22259
                     /gene="nfo"
                     /locus_tag="ACHLIF_000749"
     CDS             21402..22259
                     /gene="nfo"
                     /locus_tag="ACHLIF_000749"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO9604812.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22263..23351
                     /locus_tag="ACHLIF_000750"
     CDS             22263..23351
                     /locus_tag="ACHLIF_000750"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9604813.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23407..24657)
                     /gene="nupX"
                     /locus_tag="ACHLIF_000751"
     CDS             complement(23407..24657)
                     /gene="nupX"
                     /locus_tag="ACHLIF_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO9604814.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24757..25698)
                     /gene="rihB"
                     /locus_tag="ACHLIF_000752"
     CDS             complement(24757..25698)
                     /gene="rihB"
                     /locus_tag="ACHLIF_000752"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO9604815.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25828..26526
                     /gene="yeiL"
                     /locus_tag="ACHLIF_000753"
     CDS             25828..26526
                     /gene="yeiL"
                     /locus_tag="ACHLIF_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO9604816.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26594..27844)
                     /locus_tag="ACHLIF_000754"
     CDS             complement(26594..27844)
                     /locus_tag="ACHLIF_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO9604817.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27938..28876)
                     /gene="psuG"
                     /locus_tag="ACHLIF_000755"
     CDS             complement(27938..28876)
                     /gene="psuG"
                     /locus_tag="ACHLIF_000755"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO9604818.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(28864..29805)
                     /gene="psuK"
                     /locus_tag="ACHLIF_000756"
     CDS             complement(28864..29805)
                     /gene="psuK"
                     /locus_tag="ACHLIF_000756"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO9604819.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(30229..31920)
                     /gene="fruA"
                     /locus_tag="ACHLIF_000757"
     CDS             complement(30229..31920)
                     /gene="fruA"
                     /locus_tag="ACHLIF_000757"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO9604820.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(31937..32875)
                     /gene="fruK"
                     /locus_tag="ACHLIF_000758"
     CDS             complement(31937..32875)
                     /gene="fruK"
                     /locus_tag="ACHLIF_000758"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO9604821.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32875..34005)
                     /gene="fruB"
                     /locus_tag="ACHLIF_000759"
     CDS             complement(32875..34005)
                     /gene="fruB"
                     /locus_tag="ACHLIF_000759"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO9604822.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFTEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34373..35554
                     /gene="setB"
                     /locus_tag="ACHLIF_000760"
     CDS             34373..35554
                     /gene="setB"
                     /locus_tag="ACHLIF_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO9604823.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35551..35805)
                     /gene="yeiW"
                     /locus_tag="ACHLIF_000761"
     CDS             complement(35551..35805)
                     /gene="yeiW"
                     /locus_tag="ACHLIF_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO9604824.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            35960..36532
                     /gene="yeiP"
                     /locus_tag="ACHLIF_000762"
     CDS             35960..36532
                     /gene="yeiP"
                     /locus_tag="ACHLIF_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO9604825.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDIQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36755..38221
                     /gene="yeiQ"
                     /locus_tag="ACHLIF_000763"
     CDS             36755..38221
                     /gene="yeiQ"
                     /locus_tag="ACHLIF_000763"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO9604826.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38339..39325
                     /gene="yeiR"
                     /locus_tag="ACHLIF_000764"
     CDS             38339..39325
                     /gene="yeiR"
                     /locus_tag="ACHLIF_000764"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO9604827.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39364..40077
                     /gene="lpxT"
                     /locus_tag="ACHLIF_000765"
     CDS             39364..40077
                     /gene="lpxT"
                     /locus_tag="ACHLIF_000765"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO9604828.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40489..41055
                     /gene="mepS"
                     /locus_tag="ACHLIF_000766"
     CDS             40489..41055
                     /gene="mepS"
                     /locus_tag="ACHLIF_000766"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO9604829.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41236..42792
                     /gene="pdeN"
                     /locus_tag="ACHLIF_000767"
     CDS             41236..42792
                     /gene="pdeN"
                     /locus_tag="ACHLIF_000767"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO9604830.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            42874..44688
                     /gene="yejA"
                     /locus_tag="ACHLIF_000768"
     CDS             42874..44688
                     /gene="yejA"
                     /locus_tag="ACHLIF_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO9604831.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDTFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            44689..45783
                     /gene="yejB"
                     /locus_tag="ACHLIF_000769"
     CDS             44689..45783
                     /gene="yejB"
                     /locus_tag="ACHLIF_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO9604832.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45783..46808
                     /gene="yejE"
                     /locus_tag="ACHLIF_000770"
     CDS             45783..46808
                     /gene="yejE"
                     /locus_tag="ACHLIF_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9604833.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46810..48399
                     /gene="yejF"
                     /locus_tag="ACHLIF_000771"
     CDS             46810..48399
                     /gene="yejF"
                     /locus_tag="ACHLIF_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO9604834.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(48403..48747)
                     /gene="yejG"
                     /locus_tag="ACHLIF_000772"
     CDS             complement(48403..48747)
                     /gene="yejG"
                     /locus_tag="ACHLIF_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO9604835.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49080..50270)
                     /gene="bcr"
                     /locus_tag="ACHLIF_000773"
     CDS             complement(49080..50270)
                     /gene="bcr"
                     /locus_tag="ACHLIF_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO9604836.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(50298..50993)
                     /gene="rsuA"
                     /locus_tag="ACHLIF_000774"
     CDS             complement(50298..50993)
                     /gene="rsuA"
                     /locus_tag="ACHLIF_000774"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO9604837.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51142..52902
                     /gene="radD"
                     /locus_tag="ACHLIF_000775"
     CDS             51142..52902
                     /gene="radD"
                     /locus_tag="ACHLIF_000775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO9604838.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGNEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            53027..53311
                     /gene="rplY"
                     /locus_tag="ACHLIF_000776"
     CDS             53027..53311
                     /gene="rplY"
                     /locus_tag="ACHLIF_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO9604839.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53450..54457)
                     /gene="yejK"
                     /locus_tag="ACHLIF_000777"
     CDS             complement(53450..54457)
                     /gene="yejK"
                     /locus_tag="ACHLIF_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO9604840.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54639..54866
                     /gene="yejL"
                     /locus_tag="ACHLIF_000778"
     CDS             54639..54866
                     /gene="yejL"
                     /locus_tag="ACHLIF_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO9604841.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54886..56646
                     /gene="yejM"
                     /locus_tag="ACHLIF_000779"
     CDS             54886..56646
                     /gene="yejM"
                     /locus_tag="ACHLIF_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO9604842.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56721..56797
                     /locus_tag="ACHLIF_000780"
     tRNA            56721..56797
                     /locus_tag="ACHLIF_000780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56755..56757,aa:Pro,seq:ggg)
     gene            57016..58257
                     /locus_tag="ACHLIF_000781"
     CDS             57016..58257
                     /locus_tag="ACHLIF_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO9604843.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            58356..58586
                     /locus_tag="ACHLIF_000782"
     CDS             58356..58586
                     /locus_tag="ACHLIF_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9604844.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            58754..58960
                     /locus_tag="ACHLIF_000783"
     CDS             58754..58960
                     /locus_tag="ACHLIF_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9604845.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLSWQKEREEEARGTAA"
     gene            58975..>59296
                     /locus_tag="ACHLIF_000784"
     CDS             58975..>59296
                     /locus_tag="ACHLIF_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9604846.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVCDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSV
                     IVLSC"
BASE COUNT        14674 a        15385 c        14631 g        14606 t
ORIGIN      
        1 ctgaataagc gattcattgt ctctgataaa ctgcgattcc actattatcg cagcttatcg
       61 atatcaattg cctgaatagc ccttgcaata tcaggggaat tattcaacac ccgaacatgc
      121 tgaaataatt ccgttgcttc atcgtattct ttacgcaaat aactcaacca ctgtttaatc
      181 cgcgcaacgt gatataaccc ggtatcgccc tgcttttcca gacgggtata tttttgcagc
      241 aaagcaacca cctccggcca cggcattcgc ggttcgttat attttactac ccggctcagg
      301 ttgggaatat tgagcgcccc gcgaccaatc atcactgcgt cgcagccgct gatcgcaatg
      361 cattgttgcg cgctctgcca gtcccagatt tcgccgttgg caatcaccgg aatattcagc
      421 cgctggcgaa tctcgccaat cgcctgccag tcaatatgct ccgcgcggta accctgctct
      481 tttgtccgtc catgcaccac cagctccgta gcaccagcct gttgaaccgc atcggcgatt
      541 tcaaatttct tctcaccgct gtcccagccc agacgcactt tcacgctgac gggcaaatgc
      601 gccggtacag cttcacgcat cgcttttgca ccctggtaga tgagttcagg atctttgagt
      661 aacgtcgccc caccaccgct accgttgacc gttttcgacg ggcagccgca attgagatcc
      721 acgccccagg aacctaattc caccgcacgg gcggcgttct ctgccagcca ttgtgggaac
      781 tgacccaaca actgcacgcg caccagcgta ccggatggtg tccggctggc gttttgtagc
      841 tcagggcaaa tgcgatgaaa aacttttacc ggcagcagtt gatccaccac gcggacaaac
      901 tcggtgatgc acagatcgta gtcgttaact tcggtcagca attcacgcac cagagagtca
      961 agcacaccct ccatcggagc cagtaacaca cgcatatctt cacccgcaaa aaaatgaggc
     1021 gctatgttag cgcctatggt caacggatta aaggtctgca aagagggatt gctgcaaact
     1081 gtaatccctc tctatgcttg atgtatgcct gagttcgttg cgcattgcgt aatgccgggg
     1141 agcgaaaaaa taagcgctga tttcttaatg tgatcggtag cacgttttac gaattaattg
     1201 tatgatgaat ccatctcatc tggggtgttg attatgagta agacactgaa cattatctgg
     1261 caatatttac gcgctttcgt cctgatttat gcctgcctgt atgcaggcat tttcattgct
     1321 tccctgctac cggtaaccat tccgggcagc atcatcggta tgctgatcct gtttgtcctg
     1381 ctggccttgc aaattcttcc ggcaaaatgg gtcaatccgg ggtgctacgt actgattcgc
     1441 tatatggcgc tattgtttgt gccgattggc gtaggcgtca tgcaatattt tgatttgctc
     1501 cgcgcacagt ttggcccggt agtggtttcc tgtgcaatca gtacgctggt ggtttttctg
     1561 gtggtgagct ggagttcgca actggtacac ggtgaacgta aagtcgtagg tcagaaagga
     1621 tcagaagaat gatggcaaat atctggtggt cattaccgct gacgttgatt gtcttttttg
     1681 ccgcccgcaa actggcggca cggtataagt ttcccctgct taacccgttg ctggtagcaa
     1741 tggtggtgat cattcctttt ttaatgctga ccggcatctc ttacgacagc tactttaaag
     1801 gtagcgaagt gctaaacgat ctgctgcaac cggcggtcgt cgcgctggcc tatcctttat
     1861 atgagcagct acaccagatc cgcgcgcgct ggaaatcgat catcaccatc tgttttatcg
     1921 gcagcgtggt tgcgatggtg acggggactt ccgtggcatt gttgatgggc gcttcaccgg
     1981 aaatcgccgc gtcaatcctg ccgaaatcag tcaccacgcc tattgcaatg gcggttggcg
     2041 gcagtattgg cggtattccg gcaatcagcg cagtttgcgt gattttcgtc ggcatcctcg
     2101 gcgcggtatt tggccataca ttgcttaatg cgatgcgtat tcgtaccaaa gctgcgcgcg
     2161 gtctggcaat ggggactgcc tcgcacgccc tcggtacggc gcgctgcgcc gagctggatt
     2221 atcaggaagg tgcatttagt tcgctagcgc tggtgttatg cgggataatt acttcgctga
     2281 tcgcaccgtt ccttttcccg attattctgg cagtaatggg ctaaaatttg cgatgcgtcg
     2341 cgcatttttg atgtaagttt cacgcgttgc ataattaatg agattcagat cacatataaa
     2401 gccataacgg gttcgtaaac tgttatccca ttacatgatt atgaggcaac gccatgcatc
     2461 cacgttttca aaccgctttt gcccaacttg cggataactt gcaatctgca ctggaaccta
     2521 ttctggcaga caagtacttc cccgctttgt tgaccgggga gcaagtctca tcgctgaaga
     2581 gcgcaacggg gctggacgaa gacgcgctgg cattcgcact acttccgctg gcggcggcct
     2641 gtgcgcgtac gccattgtcg aattttaatg ttggcgcaat tgcgcgcggt gtgagcggaa
     2701 cctggtattt cggtgccaat atggaattta ttggtgcgac aatgcagcaa accgttcatg
     2761 ccgaacaaag cgcgatcagc cacgcctggt tgagtggtga aaaagcgctt gcagccatca
     2821 ccgttaacta cacgccttgt ggtcactgcc gtcagtttat gaatgaactg aacagcggtc
     2881 tggatctgcg tattcatctg ccgggccgcg aggcacacgc gctgcgtgac tatctgccgg
     2941 atgcctttgg gccgaaagat ctggagatta aaacgctgct gatggacgag caggatcacg
     3001 gctatgcgct gactggtgat gcgctttctc aggcggcgat tgcagcggca aaccgttcgc
     3061 acatgcctta cagtaagtcg ccaagcggtg tcgcgctgga atgtaaagac ggtcgtattt
     3121 tcagtggcag ctacgctgaa aacgccgcat tcaacccgac tctgccaccg ttgcagggag
     3181 cgttaattct gttgaatctc aagggttatg attacccgga tatccagcgc gcggttctgg
     3241 cagaaaaagc cgatgcgccg ttgattcagt gggatgccac ctccgcaacg ctgaaagctc
     3301 tcggctgtca cagtatcgac cgagtgcttc tcgcttaagc ctggtgccgg atgcggcgtg
     3361 aacgccttgt ccggcttgcc agccctctcc tggtgtcgaa attcccggca aacagtttgc
     3421 cgtttcttgc gcaaaaccag cgggtaaagt agcctgatgg aaattttcct tagatcgagt
     3481 ctcctgcatg ttaaagcgcg tgttcctcag cctgttagtc ctgatcggct tgctgctgtt
     3541 gactgtgctc ggcctcgatc gctggatgag ctggaaaacc gcgccttata tctacgacga
     3601 attgcaggat ctcccctacc gccaggtcgg tgtggtgctc ggaacagcaa aatattatcg
     3661 tactggcgta attaatcagt attatcgcta ccgcattcaa ggagcgatta atgcctataa
     3721 cagcggtaag gtaaattatc tattactgag cggcgataac gcattgcaaa gttataatga
     3781 gccgatgacc atgcgcaaag atttaatcgc tgctggtgtc gacccatcag atattgttct
     3841 cgattacgca ggctttcgta cgctggattc catcgtgcgt acacgcaaag ttttcgatac
     3901 taatgatttc attattatca cccaacgttt ccactgtgag cgggcattat ttattgcgct
     3961 gcatatgggg attcaggctc agtgttatgc cgtaccgtca ccgaaagata tgctgtcagt
     4021 acgtattcgc gaatttgccg cccgtttcgg tgcgctggct gacctttata tttttaaacg
     4081 tgaaccgcgt tttttagggc cgctggtccc tattccggct atgcaccagg tgccggaaga
     4141 tgcgcagggg tatcccgccg tcacacccga acagttactt gaattacaaa agaaacaagg
     4201 aaagtagtta tggatgtaca gcagtttttt gtcgttgccg tttttttcct tattccgata
     4261 ttttgtttcc gcgaagcatg gaaaggctgg cgcgcaggcg cgattgataa acgggttaaa
     4321 aatgcaccgg aaccggtgta tgtctggcga gcaaaaaatc ccggactctt tttcgcttat
     4381 atggtggcat atatcggctt cggaatttta tctatcggca tgattgttta tcttattttc
     4441 tatcgttaat attttctctt ttctgttaat tattgagaat aattattact tcactcaata
     4501 agctgcggat ataacattct gaaccgcagc tatttgtgaa tcctttcaca gtataaattt
     4561 ccccacgcgc atagccttaa tatcagcacg caatatttat actctaaata attcgagttg
     4621 caggaaggcg acaagtgagt gagtccccag gagcttacat aagtaagtga ctggggtgag
     4681 cgaacgcaga cgcagcacat gcaacttgaa gtatgacgag tatatttagc gttcgcctta
     4741 attactcata gcattaagga agatcacatg ccgcaacaaa attatctgga tgaactcact
     4801 ccggctttta cgcctttact ggcgattaaa gaagcctctc gctgtttatt atgtcatgac
     4861 gctccctgta gtcaggcttg cccggcacag accgatccgg ggaaatttat tcgctcaatc
     4921 tactttcgta attttaaagg cgctgccgag acaattcgcg aaaataatgc cctcggtgcc
     4981 gtttgtgcca gagtgtgccc gacggagaaa ttatgtcaaa gcggttgtac ccgtgccggt
     5041 gttgatactc ccattgatat cggtcgctta cagcgttttg ttactgattt tgaacaacaa
     5101 accgggatgg aaatttatca gcccggtact aaaacgctcg gcaaagtcgc gattattggc
     5161 gcaggtcctg ccggattgca ggccagtgta acactgacaa accagggtta tgacgtcacg
     5221 atttatgaga aagaagcgca ccccggtggc tggttgcgta acggtattcc gcaattccgg
     5281 ttaccgcaat cagtgctgga tgcagagatc gcccgtattg aaaaaatggg cgtgaccatt
     5341 aagtgcaaca acgaagtcgg taacacactc acccttgagc agctgaaagc agaaaaccgc
     5401 gcggtactgg tcaccgtggg gttatcgagc ggttccgggt tatcgctgtt tgagcatagc
     5461 gacgttgaga ttgccgtcga cttcctgcaa cgtgcacgac aggcgcaagg cgatatcagc
     5521 attccacaaa gcgcattaat tatcggcggc ggtgatgttg cgatggacgt agccagcacg
     5581 ctgaaagttc tcggctgtca ggcggtaacc tgcgtagcgc gtgaagagtt agatgagttt
     5641 ccggcaagcg aaaaagagtt tgccagcgcc cgggagctgg gcgtttcgat catcgatgga
     5701 ttcacgccag tagccgtgga aggcaataaa gtcacgttta agcatgtacg gttaccgggc
     5761 gaactgacga tggcggcaga taaaattatt ctcgccgtcg gtcagcatgc cagactggat
     5821 gcctttgcgg agttagagcc gcagcgtaac accatcaaaa cacaaaatta tcagacccgc
     5881 gatccgcaag tctttgctgc tggcgatatt gttgagggtg acaaaaccgt ggtctatgcc
     5941 gtgaaaaccg ggaaagaagc cgccgaggcg attcatcact atttagaggg agcttgctca
     6001 tgttaacgaa agatctttcg attacttttt gcggcgtgaa gtttcccaac ccgttctgcc
     6061 tctcttcttc gcctgtaggc aactgctatg agatgtgtgc caaagcctac gacacaggtt
     6121 ggggcggcgt ggtgtttaaa acgatcggct tttttatcgc caacgaagtc tcgccgcgtt
     6181 ttgatcatct ggtgaaagaa gataccggtt ttatcggctt caaaaatatg gagcagattg
     6241 ctgaacatcc gttggaagag aatctggccg ccctgcgtca gctgaaggaa gattacccgg
     6301 acaaagtatt gatcgcttcg atcatggggg aaaatgagca gcaatgggag gagctggcgc
     6361 gcctggtgca agaagctggc gcggatatga tcgagtgtaa cttctcctgt ccgcaaatga
     6421 cttctcatgc gatgggtagc gatgtcgggc aaagcccgga gctggtagaa aaatattgtc
     6481 gggcagtgaa acggggttcc acgctgccaa tgctggcgaa gatgacgccg aatatcggtg
     6541 atatgtgcga agtggcgctg gcggcgaagc gcggcggcgc agatggcatt gcggcgatta
     6601 acaccgttaa atccatcacc aatatcgatc ttaatcagaa aatcggtatg ccgatcgtta
     6661 acggaaaatc gagtatttcc ggatattccg gtaaagcggt aaaaccgatc gccctgcgct
     6721 tcattcagca gatgcgcacc catccagaac tgcgcgattt cccaatcagc ggtatcggcg
     6781 gcattgaaac ctgggaggat gcggctgagt ttttattgct cggcgcagca acgttacagg
     6841 tgaccaccgg catcatgcag tacgggtatc ggatagtgga agatatggca agcgggttgt
     6901 cgcattatct cgccgatcag ggatttgatt cgctgcagga gatggtaggt ctggcgaata
     6961 acaatattgt cccggcggaa gatttagacc gcagttatat tgtctatccc cgtatcaatc
     7021 ttgataaatg tgttggctgt ggacgctgtt atatttcctg ttacgacggc ggtcaccagg
     7081 cgatggaatg gagcgagaaa acccgcacac cgcattgtaa taccgagaaa tgtgtgggtt
     7141 gtctgctttg tggtcacgtc tgcccggtgg gttgtattga gctcggggaa gtgaagttta
     7201 agaaaggcga gaaagaacac ccggtaacgt tgtaaaacca gagacgcatc cggcatttgg
     7261 caccgattgc cggatgcagc gcatccggca atatccacaa tcacatcact tcttacgcgc
     7321 gtatttcagt gaatccagcg ctacggcgaa gataataatc gcccctttga tgatgtactg
     7381 ccagtatggg tttacgccga tatacgtcag gccatagttg atgacggtaa aaataattac
     7441 cccggtcacc acgccaatca ccgtccccac accgccgctg aacgatacac cgcctaccac
     7501 gcacgccgcg atagcatcca gctcatacat aaagccgagg ttgttggtgg cagagccgat
     7561 acgtccggct tctaacatcc cgccaaaggc atagaacacg ccagacaacg cgtagatcat
     7621 cagcaggttc aggccgacgt tgacaccaga tacttttgcc gcttccgggt taccgccaat
     7681 ggcaaaaatg ttcttaccga agcgggtttt gttccacaac acccagacga acgccaccgc
     7741 aatcaacgcg tagaaggtga tgtaagagag acggaaactc cccagcgcga caaagccctg
     7801 agcaaaggta gagaagccac tgtcaaaacc agaaattggc gacgccccga caaagtcgta
     7861 atagagcgag ttgattccat agacgatgat catcgtgccg agcgtggtaa tgaacggcgt
     7921 cacgttgaga taagcgataa tcagaccgtt gatcaaaccg atcaccgcac caatggcaca
     7981 gacaatcaga ataaccagcg caatcggcat cgtcgccatt tccgggaaca ctttgttggc
     8041 gttatccatg gactgcaata atgtcgcagc caccactgcc gccagcccta cctgacgacc
     8101 agcagaaaga tcggtcccct gggtgacaat taaccctgcc acaccgagcg cgataataat
     8161 acgcaccgat gactgggtga gaatattact taagttcaac agacttaaaa atgttgggtc
     8221 ctggaaaata ataatcgcca gcaaaactaa aagaacgacg taaataccgc cctctttcag
     8281 gtaagtaaga aaacttttct tatttaacgc actcatggga agcccctaat cttaaaggtg
     8341 caaagacgca agacgcagaa tttcgttttg cgttgttgtt tttgtatcga caattccgga
     8401 aacgagacca ttgctcatga ccagaatacg gtctgttatc cctaacaact caggcatttc
     8461 agaggagata ataataatcc ccttgccttt cttcgccagt tcggcaatta actgataaat
     8521 ttcaaacttc gccccgacat caataccgcg cgtcggttca tcgagcatta atatttctgg
     8581 ttgcgttagt agccagcgac caataatcac cttttgctga ttaccaccgg agagcgaacc
     8641 aatttgcgtc cgatgacccg gcgtttttac ccgcatcgaa tcaatcaccc actgggtatc
     8701 gcttttcatc cgcgagttat ccagtaaacc aactttattt ttgtagttgc gaatattgga
     8761 aattaacgag ttaaaactaa tatccagata ggcataaatt cccgttgagc ggcgctcctc
     8821 agttaccagt gcaaatccgt ggtttatggc ttcgttggca ttatggttat tgatctgttt
     8881 gccgtgcaac gtaatggtgc cagccgattt ctcgcgaata ccaaataacg tctcaacaat
     8941 atcggtacgt ttcgccccca ccagcccggc aataccgagg atctcccctt tatgcagatc
     9001 aaacgagaca tcgcgaatcg acggctggcg cagtgacgtt aggttacgca cctcgaggat
     9061 gacttcgccc ggcttgtttt ctttgtcagg aaaacgctga ttaagagaac gcccaaccat
     9121 catggcgatg atcttgtcca tcgtcagtcc tgccagcggt tcggtggcga tccactgacc
     9181 gtcgcgcaat acggtaactt catcacataa ctggaagatt tcttccattt tatgcgagat
     9241 ataaacaata ccgcagccgc gctcttttaa tttacgaata atagtgaaca gatgattgac
     9301 ctctttttcg gttaacgaag aagtcggttc atccataatc acaattttcg cgttatagga
     9361 aaacgctttg gcgatttcga tcatctgcat ttgcgaaacg gataatgtgc cgacgcgcgc
     9421 acgcggatcg atatcaatat ccagttcatc aaaaatcgct ttggtttcgc ggtacatttt
     9481 gtcctgatcg acaaacatgc ctttggtggg atatcgcccc agccacatgt tatccatcac
     9541 cgaacgttgt aataccaggt ttaactcctg gtgtaccatc gaaataccat tttccagtgc
     9601 ctctttggcg gaatggaaat cgatctcttt accctggaat aaaatggtgc cggagtcttt
     9661 ttgataaata ccaaacaggc attttaataa tgtcgatttt cctgcaccgt tttcccccat
     9721 taatgcatgg atagaatgtg gccggacttt taaattaacg ttatcaagtg ccttaacacc
     9781 aggaaaggac ttgttgatac cgctcatttc caacaagtat tccccggaag acggagtcgt
     9841 tgagctgacc atataatttt accttgttgg ccatacaata agggcgcagt aaacgactgc
     9901 gcccaatcag tcttatttct tgctgaattc agccaggttg tctttatcta cgccaacata
     9961 aggtacgcgg accactttgt tgtcgatttt ccagttggtg ccatcagccg cacctttgcc
    10021 atcggccagg tttttcgcca gatcaaaggt cgctttcgcc tggttgttag catcgttcag
    10081 tactgtgccc gccagtgcac cggatttcac cagcgccagc gcttctggca gcgcatcgac
    10141 gccaaacacc ggaatgctgg acttgttgtg tgctttcagt gcttctaccg cgcccattgc
    10201 catcgcatcg ttgttggcga taaccacttc gattttgttg gcgttcgggc cagacagcca
    10261 ggcgtccatc ttatctttcg cctgagcggt atcccacata gcggtatcta actgtaactg
    10321 ttcagtttta atgcctttgt cgttcagctc tttaatcacg taagtggtac gtgcttctgc
    10381 atccggatgg cccggttcac ctttcagcaa tacgaattga atctgaccgt ctttgttcag
    10441 atcccaaccc tgattcgccg cccagtgttt agcaatcaaa tcgccctgaa taatgccgga
    10501 ctctttggaa tcagtgccaa cgtagtaggc tttgtcgtag ctatccagcg ccttacgaga
    10561 cggttctttg ttgaagaaaa ccaccggcac gttttgccca cgcgctttct caatcaccgt
    10621 acccgcagct gccgggtcaa ccaggttgat tgccagtgcc ttcacccctt tcgccagcaa
    10681 tacgtcgatc tgatcgttct gcttggactg gtcattctga gaatcattca tcagcagctg
    10741 aacatctggc gcggctttcg catcttgctc aatagccttg cgcactacag acataaagtt
    10801 atcgtcgtac ttatagattg ttacaccaat gcgagtatca gcagcgtgtg cagcggcacc
    10861 gaataacatg ctggccatca cagcagacag ggttaacacc ttcttattca tggtatctcc
    10921 ggtttttctt atgcagggta gtgcttgaga taaatgctcg gcggggcagt agagttaatg
    10981 aagtgttact gaacgccgaa gctcactttt taaaattcgt tcttccatgc ccggtaacgc
    11041 tccagaaaac ggctttaatt gttgttatga cgctattacc tcggtgaaag tgattattca
    11101 ctgttacata cgggttacaa cgttaaaacg gtgcaatcat agctatcaca ttgttaagat
    11161 actgtgaaat cactcacaga ttgaaagcgg ttacatcgcc tgatttgttg agttagtgat
    11221 cgtcgccgca ttctggcgtg ttgcgacaga atggcgacgc actaacgtcg gcataaaaca
    11281 gtggctagca cgaggatcaa tattgcctgc tgccccctgc aaggccagtt cggtggctaa
    11341 tttcgccatt gaagcaatgg gataacgcac ggtcgttaat tgcgggtcgg tgtaacgggc
    11401 gatgggaata tcatcgaaac cgatgattga gagatgtaac ggaatcgcaa tgccattatc
    11461 ttttaatgct gtcagtgcgc cagcagccat attgtcgtta taagcaaata cagcggtaag
    11521 ttgtagattg cgccccagca gttcaaccat cgccgcctca ccgcccggca tgtccggcgt
    11581 accagtgcca atccagcttt ccggcggaat aatatcctgc tctttcaacg cactcatcca
    11641 gcctgcttta cgcatggcgt catcttcaat gccgtgactg gaagaaagat aaccaatacg
    11701 ttgatgaccg ttattcagca acatgcgcgt cgccattcgg gcaccgctga gattatccag
    11761 gcaaacgcaa cgatgggcgt accccggcac aacgcggttg attaacacca taccgggaat
    11821 gttatccata aattgcgcca gttcatcgtc actcaatgct tttgagtgaa caatcaacgc
    11881 attacaacgc tggcgaatta acacctcaat ggcgtgacgc tctttttccg cttcatgata
    11941 gctattgccg attagcacgt atttctgatg ctgctgagcg accagatcca ccgcttttac
    12001 cagcgcgccg aaaaacgcat cagaaacgtc catcaccacc acgccaatgg tgtcgctaac
    12061 ctgagttgcc agcgcctggg cattggcgtt tggccgataa tccagctcac tcacggcttt
    12121 cattactgct tcacgcgtgt cggcactgac cagcgtgctg ttattgagca cccgggaaac
    12181 cgttgccaca gagacgccag cctgacgcgc tacatcacga atggtgatca tattcactac
    12241 ctgttatgag attgcagtaa atgactgctt gctggcggct attttgtcag cactgaatac
    12301 aggacttcgt gaatcgagtc acagcaatgg aaacggttac agccgttttg ttaacgaatg
    12361 tgatccagat cgttatcttg atgttttaga tattgccgtt ccggcagcac gcaaagttaa
    12421 ctgacgccag agccattcca ccggcccctg gcggaagtaa cgcagccaga taacagagaa
    12481 gaggatattc gccagccata ccggaataac aaacgccagc agctccaggc ggtcaaaatg
    12541 cataaacaaa ccgaggtggt aaaaaagcat ggtacaaatc agcgtttgca atagatagtt
    12601 ggtcagcgcc atccgtccga cgcaggcgat cgcaagcacc agcttaaagc ggctcaattg
    12661 cggccagaag ccataaaaca gcgacgcata gccaatcgcc tgaaacggcg cactcagttc
    12721 ccgcggcatt tgaagtaaga aggcacacca gcgatacgcc cagtccagct gccattgcag
    12781 ggcgatggca ggaaggttaa tggtcacccc aatcgccacc agcacaaaac cagtacgacg
    12841 atagtgacgt aagctgaact gccctttcag ccagccgctg cgcatcagcg ctgcaccaat
    12901 gagcatcatc ccagccagtt gccagccata ctgtgcgccc agtgccagta aactgttgcc
    12961 aacaccatcg gcacgattac tgatcgcttc aacgccgccg tgaagcttcc agtatttttc
    13021 atacaaaata gccgatgcat ccggcgtcca ggcgcggctg gtctggctgt cggaaatcag
    13081 ccccaataac agcaaaacgc caagccccac cagataaagc atgacgcctg tattaaacag
    13141 gctttttacc gatggcgcat cgcgcaccag ccgccagcag attaagccca ccagcccgta
    13201 agccagcaga atatcgccgt cccagaacaa taaaccgtga ataaagccca ataagactaa
    13261 cagcgttaac cgcgactgga tccagcgtct gccacggggc agcaacattt gcagtccagc
    13321 accaaacagc agcgcaaaaa gcgtgaggaa tttcacctgg gcgatgagat caagaaatgc
    13381 ccaggtccat gcatcctgcg gcgtaatagc accgtaccag gcgggattga gataagccgc
    13441 ctttggtagc ccaaaggcgc tgatgtttaa tagcaggatc cccagaatgg cgacgccgcg
    13501 aacaaaatcg agcgtgacgt tgcgctccat ggttcctgcc ttttaatcag ttgtgatgac
    13561 gcacagcgcg cagaaactcg tggcgcgtat tctgactgga tttgaacaat ccaccaagag
    13621 aggtcgttgt tgtggcactg gttgcatcgc ggatgccacg cgccttcacg cagtaatgca
    13681 ccgcgtcgat cgagacagcc acgttattgg tgcccagcag cgtttgtagc gcaataagaa
    13741 tttgctgcgt cagacgttcc tgcacctgcg gacgctgggc aaagaactgc acgatacggt
    13801 taatttttga cagaccgatc accgaatctt tcgggatata agccaccgtc gctttgccat
    13861 cgatggtaac aaaatggtgt tcacaggtac tggtcagagt gatatcgcgc acggtgacca
    13921 tttcatcgac cttcattttg ttttcaatga gggtgatttt cgggaagttg gcgtaatcca
    13981 gaccggagaa aatttcatcg acatacattt tagcgatgcg atgcggcgtt tccatcaaac
    14041 tgtcatcagc caggtcgaga ttcagcagct gcatgatttc ggtcatatga ccagcaataa
    14101 ggcttttgcg cgtttcgtta tccatttcat gcacgggcgg gcgcagcggt gtttccagtc
    14161 ctcgcgcaac taacgcttca tgaaccaggg ccgcttcttt actgagtgat ggcatttatg
    14221 atttctcctg caggtgtgac gcctccgccc tgcgtggggg caaagttatt aagctgattt
    14281 acagcttgat tattgtgcgt gaggcggcgc acataatcca gtattcacag cgataattat
    14341 tgtaattgcc gctgcctttc atcagcagat gttaaaacat cgttatgcaa atacggaagt
    14401 gaaagttact cacagcacat tgaataaacg gtatgatgaa gaaattgcaa acaacacaac
    14461 aaggagccac gcatggaaat gctcgaagag caccgctgtt ttgaaggctg gcagcaacgc
    14521 tggcgacacg actccagtac cttaaactgt ccgatgacgt tcagtatctt tctcccacca
    14581 cctcgtgatc acaccccgcc accagtgctg tactggcttt ccggattaac ctgcaatgac
    14641 gagaacttca ccatcaaggc gggtgcccag cgcgtggcgg cagaactggg gattgtactg
    14701 gtgatgccag acaccagccc gcgcggcgaa caggttgcca acgatgatgg ctacgattta
    14761 ggccagggcg cggggtttta tcttaacgcc acgcaaccgc cgtgggcgac gcattaccgg
    14821 atgtatgatt atctgcgcga tgaattaccg gcgctggttc agtcgcaatt taatgtcagc
    14881 gaccgctgcg ccattagcgg tcattcaatg ggtggtcacg gtgcgctgat tatggcgctg
    14941 aaaaatccgg gtaaatacac cagcgtttcg gcctttgcgc caattgtgaa tccgtgcagc
    15001 gtcccgtggg gaatcaaagc gtttagcagc tatttaggtg aggacaaaaa tgcatggctg
    15061 gaatgggaca gttgcgcgct gatgtatgcc agtaacgcgc aggatgcgat cccgacgctt
    15121 atcgatcagg gcgataatga tcagtttctt gccgaccagt tgcaacctgc ggtactggca
    15181 gaagccgcgc gccagaaagc gtggccgatg acgctgcgta ttcagccggg atatgatcac
    15241 agttactact tcatcgcctc ttttatagag gatcacctgc gcttccatgc gcagtattta
    15301 ctgaagtgaa agtccgcccg gttcgccggg catcttctca tcagaagcga taatccactg
    15361 ccataaagta acgacgtccg tcttcgttat agctgtagtc gtcacgactg agatccttgt
    15421 cgccaaggtt cagcacgcct gcgcgcagtt tgacgtcttt agtcacctgc caggccgcgc
    15481 cggtattcca gatggtataa ccgcccggtg ttttagccgt cgcgctgtcg gcgcgtttct
    15541 gcccggtata gtgaccagaa acatagaatg accagtcttc cagcgccagc ggtttccagt
    15601 ccagcgtacc gttagcagta tggaacggca gatcggatag cggtttgttt tcgccgttgc
    15661 tgacatcacg accatcgttg taggtgtagt tgatcgacag tttccattca tcgttgaacg
    15721 gaattttcag ttcggtttcc acgccctgaa tacgagcttt gttaacgttg tagtagctaa
    15781 ataccggtat gcgccgtccg ttagcgcccg tctcaaaacc aacaaagttt tggtagcccg
    15841 gtgcagcgtt gacgtcagac gtacggctga tgctgatacg atctttcaca tcgttacgga
    15901 aaacggtaac gctggattca accccttcca gccagccttc ttcacccatg tagtaaagcc
    15961 ccagctccca actttcgctg gtttctggtt tcagatccgg gctacccaca atcttacatg
    16021 cgccacggca ggaattgctc gtccagtcag ggctaagttg caacagagaa ggtgctttaa
    16081 atgccgtcgc ccagccccct ttcaccgtta cggtgtcggt ggcgttataa accaggtagg
    16141 cacgcggact ccagtgttca ccgtaggttt cgtgatcgtc catacgcacg ccggtcgtca
    16201 gcgccagcgg ctcgaagatc cgccattcat cttccacaaa cagcgcgtac tggctggcag
    16261 acgttttgga gctggttccc ccggtcaggt tcaccgcatc gctaagtttg tcgtgacgcc
    16321 attcaccgcc aaccgtgaga aactgattaa ttgccgtcag cggcaacgtg tatttgccgt
    16381 cgaccgtatt gctttcggaa gttatcgggc tgctgttgcc agggtttttg ttctcgactt
    16441 tctcaccgta gtatttcagt tcgctggtgc cgtaatccca acgcccatta tggctgacgg
    16501 agtagttctg gcgttccagg cggtttttgt ccagcgagtc ggaatcacga tcctgacggt
    16561 cgaaaccgta tccggcagta aaatcgtgat tttgattcgg tgtccaggca aattcgacat
    16621 tgccgtcgcg gctggagaat ccttcaatac gcggcgtttc tccggtatcg gtggtcgttg
    16681 agttttgcgg gtcatccttt tcacgttttg ccaggctgcc gtaagctttc attcccagca
    16741 caccatcaat taatggtcca ctggtaaaga actgaccgtt ataggtatca ccgcgatcgc
    16801 gatgttcctg aatggtggta tcgacggtaa cggtacccga ccatttctga ccgatttttt
    16861 tggtgatgat attcactaca ccgccgagcg catcggagcc gtatagcgac gacatcgggc
    16921 cacgaaccac ttcaatacgt tcgatggaat cgaccgggat ccagttcaga tcgaaatcat
    16981 tgtggcggaa gacggcattg cgggagttaa cgcgtttacc gtcgacgaga atcagggtgt
    17041 agctgctgtc cagaccacga atactaacgc ccttacggtt atccccttcg ttcgtcagtt
    17101 gtacgccagg cacttctttg aggacatcct tcagattctg taccggtttt cgctgcaggt
    17161 cttcctgggt aatgacgctg atactggcgg gtgcatcttt gaggttttgt tcaacggaag
    17221 atgcagtgac aaccatcgtt tcgccatcat catcgaccgc taacacaggc catgcacaag
    17281 aaatagcgga caaacacagc ccgacccgta cgaaagggtt caacctaaac attccatatc
    17341 tccatgaggt aactacgaaa ataaaatggg ttatcgctca catcttcttc acgtcccctt
    17401 gcgtacggca gcatcgcggt ggacttattt gtatgcaggt gatcatccca gaaagccgga
    17461 acagcctctg atttgtcagc cttttttgat tgcggcgtaa cgataatgca aacgataaca
    17521 attatcaatc caaatgttaa attttatatc cgcatgattt gtcagggaaa tggaattgaa
    17581 aaagccctct cggttgagag ggcttagcaa ggaagggagg aaacttattt cttatcgttc
    17641 tgcgggaact tcatttcgct gtagcgtacg aagtgagttc ctttaatcag cttgtagccg
    17701 aaccaaataa tcaggaacag cgggatacca atatacgttg ccgctacgcc gccccagtca
    17761 atggtatctt tcaggaacgc ttcgtagttc tggcccaaag tgataatcag acacagaatg
    17821 aatgcgaaga tcggccccag tgggaagaaa cctgaacggt acggcagatc gttaatgtcg
    17881 tgtccctgca atacgtaacc gcgacggaag cgatagtggc taatggcaat ccccagccag
    17941 gcgataaaac ccgtcatccc ggaggtgttc agcagccaca ggtataccgt ctggttgcca
    18001 aacatggagg tcaggaagca cagaccggca atcaccgtcg tcgcatacag cgcattacgc
    18061 ggcacgccac cacgcgacag tttagcgaaa atgcgcggcg ctttaccgtc acacgccagg
    18121 gtgtacagca tacgagtaga cgcatacata ccggagttac ccgctgacag caccgccgtc
    18181 agaataactg cgttcatcac cgccgccgca gagagcagac ccgcgtgctg gaacaccagg
    18241 gtgaacggac taacgctgat gtctttaaca tcgttacgca gcaggctcgg atcggtgtac
    18301 ggaataatca ggctgataat caggatcgcg aacacataga acaacaggat tcgccagaac
    18361 acctgacgta ccgcgcgtgg aatgtttttc gccggatctt cggactcgcc tgcagcaata
    18421 ccgatcagct cggttccctg gaaagagaag ccgacaatca tagctacgcc gatcatcgcc
    18481 gcaaaaccac cagcaaacgg cgcttcgccg attgtccagt tgctccagcc cgcaggctgc
    18541 gcgcctttga agataccgat aatcatcagc acgccaacga tgataaagac aataactgtc
    18601 gtgactttga tcagtgagaa ccagtattcc gcttcaccaa agccacgaac tgagatgtag
    18661 ttcagcagga agataacgcc gaggaacaac gcactccaga tccagcccgg tgtatccggg
    18721 aaccaccagc tcatgaccag ctgagctgca accaggtcaa cggcgatagt caccgcccag
    18781 ttgtaccagt agttccagcc cagcgcgaag ccaaagcctt cttcaacata gttctgaccg
    18841 taagtggcaa acgaaccgga aaccggcata tatgcagcca gttcaccgag actggtcatc
    18901 aggaagtaaa ccatcaggcc aatcagcata tacgagagca atgccccgcc cggacctgcc
    18961 tgagaaatcg ttgcgccaga ggcaacaaaa agacctgtac cgatggaacc gccaatggca
    19021 atcatcgtca ggtgacgcgc ctttaattca cggcgtaagc ccggcgcttc tgtggtttta
    19081 gtttcggaaa ccatacaaaa atgctatcca tcttaaaaat gaggcgcgat tgtagcagac
    19141 gattggcaat ccttccggca gaaatacacg gttataagac accttcatga tcgcccaggg
    19201 attataagta aagcagccaa tctcttttct ggcgaatgca tgctgagggc gctgcgtcac
    19261 gaaatcgaca cagcaccagc atgttcttgt acagcaactt aacgcggtac attcgcggga
    19321 tcgcaatagt ccagaaagcg ccgtagcgcg ttggaaaggt gtttttgccg atgatgtata
    19381 cgccacaacg tacgcatcag gcgcgggaga gggaccgcaa cttcacttaa tgtgcctgcc
    19441 tgcaattgat ctttaatcac ccgtcgcgac aggcaactaa ttcccaaccc atgacgcacc
    19501 gcatgtttga ttgcctcgga gttacctaat tccatcgcca tctcaaactt cggtaaatgt
    19561 gacagcaaca gataatcgac aatctcccgc gtgccggaac cgcgttcacg caggatccac
    19621 ggcgcagcgg ccagctgttc taaggtgacc ggaccacgag ccaacggcga agtcggcgcg
    19681 gcgaaaacca ccagctcgtc ttccagccac ggttcagaaa tgatttcagt gctgtggcac
    19741 ggtccttcaa taaagccaat atcaacgcgg aaatccagca ccgcttgcat cacgtcctgg
    19801 ctgttcccaa cgctaagttc aatcggcaac tgcggataat catggcgata acgggcgata
    19861 actgcaggca gaatgtagtt accgatggta ctactggcat agatacgaat cgcgccgttg
    19921 tcttcgcgaa acagttgttc gatttcaacc gcctgttcaa gcaatgccaa cgcacgcgga
    19981 tagagcagcc gcccgtgttc attaacaacc agtcttttcc ccacgcgatc aaacagttgc
    20041 acgccaagct gcccttccag gtcggtcaag gctgcgctca ctgctgattg cgacaacgcc
    20101 agcatcaccg acgcctgggt ggttgatcca cttttcaata cttctgcaaa aacttccaac
    20161 tgccggaggg tgatgtgcat agtcgcttac cacttataaa gattaattat aaatatataa
    20221 tcaattttat ttttaaacca gttagtcgta accttatgcc cggttaaagg agagggttat
    20281 gacgaatatc accttacaga aacaacatcg tacactgtgg cattttattc cggggttagc
    20341 cctgagtgca gttatcaccg gggtcgccct gtggggtggt tccattcccg cggttgcggg
    20401 tgccgggttt agtgccctca ccctcgcaat cttgttgggg atggttttag gcaacaccat
    20461 ctatccgcac atctggaaaa gctgtgacgg tggtgtgctg tttgccaagc aatatttatt
    20521 acgtctgggt atcattcttt atggcttccg tctgacgttc tcgcaaattg ccgatgtcgg
    20581 tatcagtggg atcatcattg acgtgttgac gctgtccagt accttcctgc tggcttgctt
    20641 cctggggcag aaagtgtttg gtctggataa gcacaccagc tggttgatcg gtgccggtag
    20701 cagtatctgt ggtgctgccg cggtactggc gactgagccg gtagtgaaag cggaagccag
    20761 taaagtaacc gtggctgttg caaccgttgt tatcttcggg accgtcgcta ttttcctcta
    20821 cccggcgata tatccgctga tgtctcaatg gtttagtccg gaaactttcg gtatctacat
    20881 tggttctact gtgcacgaag tggcgcaggt ggtggcggca ggtcatgcca tcagcccgga
    20941 tgcggaaaac gcagcagtta tttccaaaat gctgcgcgtg atgatgctgg ctccgttcct
    21001 catcctgctg gcggcgcgtg ttaaacagct gtctggggcg aacagcggcg agaaaagcaa
    21061 aatcactatt ccgtggtttg ccatcttgtt catcgtagtt gccatcttta actcgttcca
    21121 cctgttaccg cagagcgtgg tgaacatgct ggtaacgctg gataccttcc tgctggcaat
    21181 ggcgatggcg gcgctgggtc tgaccactca cgtcagcgcg ctgaaaaaag ccggggcgaa
    21241 acctctgctg atggcactgg tactgtttgc ctggctgatt gttggtggtg gtgcgattaa
    21301 ctatgtgatt caaagcgtca tcgcataaac cactacatct tgctcctgtt aacccgctat
    21361 cattaccgtt ttcctccagc gggtttaaca ggagtcctcg catgaaatac attggagcgc
    21421 acgttagtgc tgctggcggt ctggcaaatg ccgcaattcg cgccgccgaa atcgacgcaa
    21481 ccgcgtttgc cttgttcacc aaaaaccaac gtcagtggcg tgccgcaccg ctcacgacgc
    21541 aaaccatcga tgaattcaaa gcggcctgtg aaaaatatca ctacacatcg gcgcaaattc
    21601 ttccccacga cagctatctg attaacctcg gacatccggt cactgaagct ctggaaaaat
    21661 cgcgcgatgc ctttatagat gaaatgcagc gttgcgaaca actggggctt tctttgctca
    21721 acttccaccc tggcagccat ctgatgcaga tttcagaaga ggattgcctt gcgcgtattg
    21781 ccgaatccat caacattgcg ctggataaaa ctcaaggtgt aacagcggtg attgaaaaca
    21841 ccgccggtca gggcagtaac ttagggttta aattcgaaca tctcgcggcg attatcgacg
    21901 gcgtggaaga taaatcccgt gtcggcgtct gcattgatac ctgccatgct ttcgctgccg
    21961 ggtatgattt gcgtactcca gccgaatgcg aaaaaacgtt cgctgatttt gcccgtattg
    22021 ttggctttaa gtatctgcgc gggatgcacc ttaacgatgc gaaaagcacc tttggcagcc
    22081 gcgttgaccg ccatcatagc ctcggtgaag gcaatatcgg tcatgatgcg ttccgctgga
    22141 tcatgcagga cgaccgtttc gacggcattc cgctgatcct tgaaaccatc aacccggata
    22201 tctgggcaga agagatcgcc tggctgaaag cgcaacaaac tgaaaaagcg gtggcctgat
    22261 cgatgaacga cagggaaaag cagattctta aaattttacg gcgtaacccg ctgattcaac
    22321 agaatgaaat cgccgatatt ctgcaaatca gccgctcacg tgttgccgct catattatgg
    22381 atttgatgcg caagggtttg attaaaggca aaggatatat ccttaccgag caggattact
    22441 gtgtggttgt gggtgcaatc aatatggata ttcgcggaat ggcggatatc cgttacccgc
    22501 aagcagcctc tcatccaggt tctgttcact gttccgcagg cggcgtcgga cgtaatatcg
    22561 cgcataacct ggctctgctt ggtcgggatg tgcatttaat atcagctatt ggcaacgatt
    22621 tttacggcga gacattactg gaagaaacgc gccgcgcggg cgtgaacgtt tcaaactgca
    22681 ttcgtctgca tggacacagc actgcaactt atctcgctat cgctaataag caggaagaaa
    22741 ctatcctggc aatcaatgac acacatattc tgcaacagct aacgcctcaa ctgctcaata
    22801 cttcacggga cttgattcgc catgctggtg tggtgctggc tgattgtaat ctcacacctg
    22861 aagccctgga gtgggtcttt accattgctg atgaaattcc gatgtttgtc gataccgttt
    22921 ccgaatttaa agcgaacaaa gtaaaaaact ggtacagccg gatccacact ctgaaaccaa
    22981 cacaaaatga actggaaatt ttgtgggggc aaccgattaa agacgacaac gatcgcatca
    23041 gagctgtaaa ttcgctgcat caacaaggtg ttaaacgcat ttttgtttat ctgaaagatg
    23101 aatctgtatt ttgcagcgat aaggatggag aacaattttt gctgaccgcg ccagcgcata
    23161 cgacagtaga cagttttggt gctgacgatg gttttatggc gggcctggta tatagctttc
    23221 ttgaaggata cagtttccgc gacagcgccc gttttgcggt agcctgcgcg gcaatttcgc
    23281 gcgccagcgg cagcttaaac aaccctaccc tgtctgccga taacgcgctt tcattagtgc
    23341 caatggtgta acaatgttgc cggattcgac gcttgaccgc atcttatccg gctcataaat
    23401 tgcgcactac gctaaaccaa taaagaatcc tgcaatagta gcactcatca ggttagaaag
    23461 tgtcgccgcc gccagcgcgc gtaaaccaag ttgggcgatt tccggcgcac ggtgtggcgc
    23521 aaccgcagaa aacgccccca ccaccacccc gatagaacca aagttagcga aaccgcacaa
    23581 cgcgaaagaa ataatcgcca cggttttagc atccagagtg ccacccgttt gcagatatgg
    23641 cgagaaattg agataagcga caaattcatt gatcgccagc ttctgcccaa tcaaactccc
    23701 ggcaagattt gcatcactcc agtcaacccc catcacccac gccaatgggg ccaacaggta
    23761 acctaaaatg gactccagcg aggcatgtgc aaaaccaaac cagccgccaa cgccgccgat
    23821 aataccatta attaacgcaa tgatggcgac aaatgccata acaactgtcg ctacaccggc
    23881 ggcgattttc agcccggtca ttgcccctgt cgcagcggct tcaataatgc tttttggcgg
    23941 tgtttcggtg aaagagaggt tattaaagga aacctgcgaa gattccgtag ccgggcttaa
    24001 caggcgggca aacaagatcc cgcctgggat cgccattaac gatgccgcca gcaaatattc
    24061 cacaggtacg cccagggcgg cgtaaccaat cattgtcgaa ccagcaatcg aggccatgcc
    24121 actacaaatc gctgtaaata attcattgcg attcagacga tcgataaagg gtttgacgat
    24181 tgccggaatt tcgttttgcc cgaggaaaat ggtagtgacc gcgacgaatg actcgatctt
    24241 gctgatattt aacgctttct ggaagatacc gccgagaatg cgaattaaaa tccccatcac
    24301 accgatgtag tagagaatac tcaccagtgc tgtaacgaag ataattgccg gtaatactct
    24361 gaaaccaaag ataaatcctg cgccatcaaa cagcgtgtcc attttcggcc cgaccagcga
    24421 accgaagata aatgcgctac ccgcgtcgct gtacgccatc actttatgca cgccaaaagc
    24481 gactttttca gcgacccaac gccctggcgg taaccaaagc ataatgccgc caatcacaac
    24541 ctgtaacact aacgccgcgc caacggtacg caggctgatc ttcttcttgt ttactgacag
    24601 caaaaatgca attgccagca ataccaccat tcccagaaca cttctcatga catccatagt
    24661 gattatctct tcatgtcata ttgggcgacg gctttctgcc catcagaaat agccgggcct
    24721 gcatcttgcg ctatcggccc ggcttgttat caacgcttaa tgggttttga tgtagccgcg
    24781 cacgcactct tcgactaagc cccagaacca gtcggtatca atagtgatgc cgactttggt
    24841 attggcgggc ttgccaagaa cgcccagctc gtcgcagacg gtgcgcccat agcaagggcc
    24901 actgttgacg tccacttcga cgtacatctc ctgggtttta atgccatcag ggttaatcag
    24961 ataaccgatg caggtggcgt cgtgcaccgg gccgccagcc aggccgtagt tttcgaactg
    25021 cgttttgaga gtgaagttca tgatgtcgct gaacagctct ccggcagggc cgcctgccct
    25081 ttccatccga gcaatcacgt ccggggtgca aacggtctgg ttggtgagat cgaggcccat
    25141 catcactaat ggaacgccag aggtgaacac cacgcgggct gcttccgggt cagcaaagat
    25201 gttgaactcg gcagatggcg tgaagttgcc tgtaccgtaa gcgccgccca ttagcacaat
    25261 ttcacggatt ttgggcagga tcgcgggttg catacgcatt gccaccgcga tatttgaaag
    25321 cggaccaacc ggcaccagag tgatatcgcc atcgcttgcc atcagggtat caatgatgta
    25381 tttcaccgcg tgggtacttt ctgcctggcg ggttagcggc tcgaataccg ggccatccag
    25441 tccggtttca ccgtgaatat tatcggcaac gatttgttga cgcataatgg gttgcggcat
    25501 ccccgcgtaa accggaacat taatctccag tttctggcaa acattcaggc cattaattaa
    25561 tgttttatca agcgtctgat tacccgctac aatagtgatg cctaataaat ctattgccgg
    25621 atgtttcgct gccatcatca ttgcaatagc atcatcatga cccggatcac aatccagaat
    25681 aatttttctc ttttccattg tttatttcct ctgtttccag ttgcgttatt ttttctacag
    25741 caaagaaaat tcgaccgggc cgatgattga atcttaccaa ccgcgtacgt atgctaaata
    25801 tgagaaatct catagcggat aaacatcgtg aaagaaatcc acaataatga tcttaagcag
    25861 caattgatga gtgaatccgc gtttaaggat tgctttttaa cggatgtttc agccgatacg
    25921 cggctgtttc attttttagc gcgtgactac attgtgcagg aagggcaaca gccgtcctgg
    25981 ctgttttacc tgacgcgagg ccgcgccagg ctttacgcca cgctagctaa tggtcgcgtg
    26041 tcgctgatcg atttctttgc cgccccctgt tttattggcg agattgagtt aatcgacaaa
    26101 gaccatgaac cgcgtgcggt gcaggctatt gaagagtgtt ggtgccttgc gctccctatg
    26161 aaacattacc gcccgcggtt attaaacgac acgctatttt tacgaaaact ctgcgtcacc
    26221 ttaagtcata aaaattatcg taatattgtt tctttaactc agaatcaatc atttccgtta
    26281 gtgaatcgcc tggcagcatt tatattactc tcgcaggaag gtgatcttta tcacgaaaag
    26341 catacgcaag cggcagagta tttaggcgtt tcttatcgac atcttttata tgttctcgcg
    26401 caattcattc acgacggttt attaattaaa agcaagaaag ggtatctaat taaaaacaga
    26461 aagcagttgt caggactggc gctggagatg gacccggaga ataaattctc cgggatgatg
    26521 cagtaaaaat tatttgcaat agcgcgattg ccggatgcaa cacttaataa gcgcgtacat
    26581 ccggcaatga aactcaagct aaaccaataa agaacccggc aatggtcgca ctcatcaggt
    26641 tggaaagcgt tgctgctgcc agcgcccgta aaccaagctg ggcgatttcc ggcgcgcgtt
    26701 ttggcgaaat agccgaaaat gcgccaacga caacaccgat agaaccaaag ttagcaaaac
    26761 cacaaagcgc aaaggagata atcgcaatgg ttttcacttc cagcgtgccg cccgtttgca
    26821 ggtatgggga gaaactcagg taagcgacga attcgttaat cgccagtttc tgcccaatca
    26881 ggctacccgc aagattggca tcactccagt ccacacccat gatccacgcc agcggtgcca
    26941 gcacatagcc aaaaatactt tccagagagg cattggcgaa accaaaccag ccaccaattc
    27001 cgccgataat gccgttgatc agcgcaataa ttgcgacaaa cgccattacc accgtcgcta
    27061 caccagcggc gattttcagc ccggtcatcg caccgctcgc cgccgcttcg ataaagcttt
    27121 ttggcggcgt ttcgctgaac gacagatttt caaatgtgac ctgtgaaggc tcggtggccg
    27181 ggctaagaat acgtgcaaac aaaataccac ctgggatcgc catcagcgat gccgctaaca
    27241 ggtagtcaat tggtacgccc attccggcat aaccaatcat catcgaacca gcaatggacg
    27301 ccatcccgct acaaattgcg gtaaacaact cgttgcgatt catgcgatcg ataaacggtt
    27361 taacgatcgc cgggatctca ttttgcccga ggaaaatagt tgtgactgcg acaaaagatt
    27421 cgattttgct gatgttaaga gctttctgga aaatgctgcc aaggatgcga atcagcagcc
    27481 ccatcacgcc aatgtagtac agcagactga tgagcgcagt aacgaaaata atcgccggaa
    27541 gtacgcgaaa ggcgaagata aaacccgcac cgtcaaacag gacatccatt ttcggcccaa
    27601 ccagcgaacc aaaaatgaag gcgctaccgg catcactgta agacatcact ttatgaacgc
    27661 ctaatgccgc ctgttctact gcccactttc ccggtgggaa gtagagcatg atgccaccaa
    27721 tagcgatttg cagcagcagt gcggctccaa ccgtgcgcaa actgatgctc tttttattca
    27781 ctgacaacag aaatgctatt gccagtaaca ccaccatccc cacaacactt ctcattatat
    27841 ccataatgat tttcccttca tgccagtaaa cccggcgtca gcgccaggtt ttggtatgct
    27901 tgatgagtac gggcgacggc tttctgcccg tcagaaatta acccgcgaga cgctggtatt
    27961 ctttggcaat ttcactcgcc agaatggcgt tgttgaacac cagctggata ttggatttca
    28021 ggctgtcacc gccggtcagc tcagcaacgc gcgccagcag gaacggtgta ctttctttac
    28081 caataacgcc ctgctcttcg gcttcggcga cggcttgatc tatggctgca ttgatagatt
    28141 cctccggcat cgcaaactgt tccgggatcg gattcgccac taccaggccg ccgttcaaac
    28201 cggtttgcca tttcactgcc attgcacgag cgatttccgt ggcactatca aggcgaatac
    28261 tgacttcaaa tgggctggta cggcagaaaa acgcaggcag cgcttttgtc tgatagccaa
    28321 ttaacggcac accgaaggtt tctaaatatt ctgttgttaa tcctaaatcg agaatagatt
    28381 tcgccccggc acaaacaacg gtgacattag tatttgccag ttcttgcaaa tcggcagaaa
    28441 tatcgaaggt atgttccgcc ccgcgatgca caccaccaat tcccccggtg gcaaatactt
    28501 taattccggc aagcgccgca ataatcatcg ttgaagccac agtggttgcg ccattttttc
    28561 cggcggcaac aacaaaaggt aaatcgcgac gactaacttt ggtcacgtta tgcccttcac
    28621 gacccagtaa ttcaatttct tctttgctta aacccacttt catcacgccg ccaataatgg
    28681 cgatagtggc aggtactgcg ccctgtttac gaatagtttc ttctacttca attgcggtct
    28741 gggcattttg tgggaacggc atcccgtgag aaataatggt cgattccagc gccacaaccg
    28801 gttttttgtt ttttaaagcg tcctgcactt ccggggaaat ttgtaataat tcaggggaaa
    28861 tttttaattc agacattctg cgttctccac taacgatata acgttggcaa tcgataaatc
    28921 ggggttattg gtgtattcac aggagagcgc cattgacgaa catccctgtg cgaaacgaac
    28981 agattcggca aacggcattc cgtctaccca acacgaagca agtcccgcca tcatggcatc
    29041 gcccgctccg gtaacattaa taacattggt tttgatcggg gcagaccagc cactttcacc
    29101 gctgatatca ctgtaataaa cgccatcgcc gcccatgctc aataccagtc ggttcaggcc
    29161 atgttgatgg aaccaggcag caacttttgc cacatctcca cgccctgaca gcgcaatccc
    29221 actcagtgtt tccgcttcaa ggcggtttgg cttcagagtg tggatctgat ttagacggtc
    29281 gcgcactttg acacatttcc acgctgaaac cggatcgaca aatacgggta cgttggcagc
    29341 attatccaga atccatgcca gtgcctcttc actgatatta cagtccgcga caatgacctt
    29401 tgccctctga ataaattcac ggtgctgtgc gagatattca gctgtaatag cgttgctaat
    29461 attcatgtca tttatagcaa ccagcatttc accggtatta tcgagtaatg ataaataact
    29521 cgacgtattt tctcccggca caatcaggca tttatcgaca taaacgccag attgattggt
    29581 ttgcgttagc agcgattgac cataaaaatc actacctact gcgctcagta accaggcttt
    29641 gttacccagc aacgccaggt tttgtgcaat atttcgccct actccaccag gtgtaaattt
    29701 tattttacct ggatttgaat ccgcataatt taatgattca tgtgaatatc cggcgacatc
    29761 aatattcgcc gaacctataa ttacgacata atccttttcg cgcatagcac gtcctctggt
    29821 aaaaaattac caaccctgtc aatccacaag aatatagata ctgcaaagac agtttaaaca
    29881 tgtgttcata tttaaacatg tgctcatagt atgtatgttt tacgtaaagt aaattgccgc
    29941 cagagaggca aagcgggcag atttgagcag gctcacaaaa tgcgattaaa tgccagaatt
    30001 acaaaagcga ggaatttgtg acgggatgca cagaacagga acactaaaaa agggcagaaa
    30061 agaatctgcc cttgaggaaa taaccttagt gtttgatgaa cgtttcaggt ttctactgaa
    30121 agataaaacc atttaaattc ataaaattac attcacaaac cggtcattct ctttgcgctc
    30181 atccaatgat acagggcagt aaatttctgc cctgtaacac accttttatt acgcggcttt
    30241 cgctactgcg tccacttccg gacgtttcag gaaggcatag gccaaacccg ccaccagcgt
    30301 accggcaata attgctacca ggtaacccag taccggcgta atagcgccag ggatcagcag
    30361 aacaaacaga ccaccgtgcg gtgccatcag tttcgcacca atcgccattg agattgcgcc
    30421 agtcagcgcc ccaccaacga tacaacacgg cagcacacgc atcggatcac gagcagcaaa
    30481 cggaattgca ccttccgaaa tgaagcacag tcccaatacc agagcggctt tgccaccttc
    30541 ctgctgcgct ttgtcgaatt tgcgacgcgc caccattgtt gccagaccca ttgccagcgg
    30601 tggcaccata cctgccgcca taatcgccgc catcgggcca taggtttgag tactcagcag
    30661 acccacaccg aatgcgtacg ctgctttgtt taccggaccg cccatgtcag tacacatcat
    30721 gccaccgagg atcgccccca gcagaaccgc attcgcagtc cccatggtct gcagccagtg
    30781 agtcaaccct tcgagaatgc cagcaactgg tttaccgatc aggtagatca tcgccagacc
    30841 gaccaccaga ctggaaatta gcggaatgat caggatcggt ttcagcgcct ccatactctg
    30901 tggcagtttc agttgcgtac tgattaactt cgcaatgtaa ccagccagga agcccgcaat
    30961 aataccgcca atgaagccag aaccggtgct gaccgccagc ataccgccaa tcagacccgg
    31021 cgtgagaccc ggacgatcgg caatggagaa ggcaatgtaa cctgccagta ccggcaccat
    31081 cagcgcaaag gctgaaccac caccaatctg catcagcgcc gctgccaacg tacccggctc
    31141 tttaaacgct tcgataccaa aagcaaaaga aagcgcgata cacagaccac ctgcaacgac
    31201 catcggcagc atgtaagaga cgcccgtcag caagtgacgg tatgcgcctg cactctcttt
    31261 cttaccttca gaggtcgccg tttgagcttt gcccgccggt tcatacggcg ttgcttcagc
    31321 aaccgcttta tccagttcct gcgcggtttt cttcagcgcc agaccggtag aggtacgata
    31381 catcggttta ccagcaaatt tcgccagatc cacttcgata tctgccgcca caatcaccag
    31441 atccgctgcg gctacttctt cgggggtgat tgcattaccc gcaccaacag aaccacgggt
    31501 ttcaactttc acccaccagc cacgtttttt cgcttcggtt tcaatggctt cagccgccat
    31561 aaaggtgtgt gctacgccag tcgggcaagc agtcaccgca actacgcgtt tcggaccgct
    31621 ggcggcaacc ggtgctgtcg cagcgaccgg cgcagtgtaa ggtttcgcat ggcctttggc
    31681 ttcactcagg aacagctcag ggtgcgcaac tgcccgggaa atatcaccca gccagacatt
    31741 tttaccgttc agcgcgctgt cattcgggat ggaatcaccg agaacaatcg ccatttctgc
    31801 gtcgttcgga ttgtcgatga tttccagttt tgcttttcgc gccgccgcgc ccagcagggt
    31861 cttcgccata taggcgcgtg cctgaccgag attagcgtca ataatcagca gcgttttcat
    31921 tatgcctctc ctgctgtcag ttaaaaggtt gtaagtcgac gcgcgccatc attgcggcca
    31981 actgcggacg atcggtaata cccacattgc tttgacttac cgccagggct gcaacagcag
    32041 tcgccagacg cagtgtgtgt tcactggatt cacgcatcag caagccataa atcaggccac
    32101 caaccataga atcccctgcg ccaacggtgc ttacgacatc gactgacggt ggtttggcga
    32161 tccattcgcc ggaggcatta acccaaagcg cgccttcggc acccagtgaa ataacaacat
    32221 gcgcaatacc ttgttcacgt agcgcatgtg cagcttcaat cacatctttc atttcaggca
    32281 gtttacggcc tgcccagatt tccaactcgc ggcggttagg tttcaccagc cacggtgccg
    32341 ctttcaaacc tgctactaac gcttcacggc tactatcaaa gataatgcaa ggacactgac
    32401 tacgcaggcg agtcatccag tcggtgaacg cttccgggct gacgcctgac ggtaagcttc
    32461 cgctgacaca gaccatatcg aactgaccga gccagctcag agaatcagtc acaaagcgtt
    32521 cccagtcggc gggggtgact tcaaaacccg agaagttgaa gtcggtcact tcgccgtctt
    32581 tttccgtcag cttaacgtta attcgggtgc gcccctgtac aacctggaaa cggttggcaa
    32641 tgcccagctc gctgaacagt tgctgaaaac catcctgatt gtctttaccc aggaagccgc
    32701 caacggtgac atcaattccc aggtctttta atactttggc cacgttgatg cctttacccg
    32761 ccgcatgcag accggtggtt ttcaccaggt tcacttcgcc gcgttcaatt tccgggcaga
    32821 aaccaacaag gtcataagcc ggattaaggg tgatagtagc aacacgtctg ctcattatgc
    32881 gccctcccca agaccagcag cgatagcgtc gccgattgct ttcagcgcct gttcagcatc
    32941 tgcaccctgg gcggtaaagc gtaggcgatg acctttctta acgccaagtg ccacaacttt
    33001 catcagacta cgtccgtttg ccggtttgcc ggtgccatca aggtttgtca cggtaatatc
    33061 actgttaaat tgtttaatgg tgttgaccag catggtacct ggacgagcat gcaggccgtg
    33121 ttcattgcgc accacaaact ccgcgcttaa cacgtcgtcg gtcggcgcat catcgctggt
    33181 cagcagcgcc agcaacgttg ccgcatccgc tttcagcaag cggtcagctt tattgtcgag
    33241 caacaaatca gcgagacgct taagaaccgc aatgggctga tcgtcattca tcgccacact
    33301 caccagcatg gctgccgttt cgccgtccac atcaaaagca tttgccgcac ggcttaccgc
    33361 aatcgcgcta cgcagattgc cttcggcgct atcgctcagc cagataccct gtccgaggtt
    33421 cagcggttgt tcattgatgg ctttggtgac gaaagtggcg tcaactgccc tcgcctcttt
    33481 cagacgcgca gcgttcagcg cctgaagagt cagcagatcg ctggcggcga tatccagcgt
    33541 cagcatttcg ttgtcgagct tcagctgctc actctgcttt tcgcccatca gcaatgcgcg
    33601 aagttcttct gctgttgttg ctgacttcag ttgttcagca acggaatcat cgctcagtac
    33661 gtgggtcagc tggcgtagca ggcccagatg ttcatccgag ctggcagcaa taccgatcgc
    33721 tacgtacgct acctgaccgt caccccaggt gacgccttcc gtgaactgaa atacctgaac
    33781 gccggttttc agcacctgat cgcgggtgtc ggtagtgccg tgtggaatag caataccatt
    33841 gccgaggaac gttgaggttt gctgttcgcg cgccagcatg ccattgacgt agccttctgc
    33901 tacgttaccg gcctgcacca gcgccgcagc gagctggcga atcgcctctt ctttgtctcc
    33961 ggccttttcg cccggatgga tgtcctgtac ggataactgg aacatagttc tcctctcttg
    34021 ctgaattgaa acgattcagc ttctatgaga aaaaaagcgc caacctggct tagggttaaa
    34081 gacaagatcg cgctgaaacg tttcaagaaa gcataatact tctgtttcag cacgcaagga
    34141 aagccgtaaa gttatgagca aaagtttgat gtgctgcaca attttttgtc atttttccta
    34201 attgctgatg ggaaaatccg ttatcagcaa tttcatttca gcagcgtcag cagccaatca
    34261 gcaacagtga aatggcattt tgattattcg tggcgctttt gatttcgctg tctgtttatt
    34321 ttctgacaag cagcgtaaac tccgcgtctt cctcttccag tgatcgacca gcatgcataa
    34381 ctcccccgca gtctccagcg cgaaatcgtt tgacctgacc tcgacggcgt ttttaatcgt
    34441 tgcctttctc accggtattg cgggcgctct gcaaaccccg acactcagta tttttcttac
    34501 cgatgaagta catgcccgtc cggcgatggt gggattcttc tttaccggca gcgctgtcat
    34561 tgggattctg gtaagtcagt ttctcgccgg gcgctctgat aagcgcggcg atcgcaaatc
    34621 gctgattgtc ttttgctgcc tgttaggcgt gctggcctgc accctttttg cctggaatcg
    34681 caactacttt gttttgctat tcgttggcgt ctttcttagc agctttggct cgaccgctaa
    34741 cccgcaaatg tttgcccttg cccgtgaaca tgccgacaaa accggacgtg aggcggtgat
    34801 gttcagctct tttttacgcg ctcaggtttc actggcatgg gtcattggcc caccgctggc
    34861 ttatgcgtta gcgatgggtt tcagctttac ggtaatgtat ctgagcgcag cggtagcgtt
    34921 tattgtttgc ggcgtgatgg tgtggctgtt tttaccgtcg atgcaaaaag agcttccgct
    34981 ggcgaccggc acggttgaag cgccgcgccg taaccgtcgc gatacgctac tgctgtttgt
    35041 catttgtaca ttaatgtggg gctcgaacag cctgtacatc atcaacatgc cgctatttat
    35101 tatcaacgaa ctgcatcttc ccgagaaact ggccggtgtg atgatgggga ccgccgccgg
    35161 gctggaaatc ccgacgatgt tgattgccgg atatttcgcc aaacgtctgg gtaagcgttt
    35221 cttaatgcgc gttgctgccg tgggtggcgt ctgtttttac gcaggaatgc tgatggcgca
    35281 ttcacctgtc attctgttgg gcttgcagct gctaaatgct atttttattg gcattctggg
    35341 cggcatcggg atgctctatt ttcaggatct gatgcccggt caggcgggtt cagccaccac
    35401 gctctatacc aacacttcgc gcgtgggctg gatcatcgca ggatcagtgg cgggcatcgt
    35461 cgccgagatc tggaattatc acgctgtgtt ctggtttgcg atggtgatga ttatcgccac
    35521 tctgttttgc ttactgcgga ttaaagatgt ttaaggcgcg gtcagcattt ccagatcgat
    35581 aagccaggtc atggcctgct gacgcgagtt accgcacatt tcagcgctgg cttgtaagcc
    35641 tgcacagact ttcggtcgca gtggcgaggt gaaaatttta cagcgttgct gttcatcaag
    35701 ctgaatgcag ggcgtattgg cgggcttgcc atctggcata ccgggaatag ggctggaaat
    35761 tgaaggggca gtgcaacacg ccccgcaacc cggacggcat tccatacgtt gttctcttaa
    35821 ggttaaccac cggattgatg agcgcgcaca gtaccacctt ttttgcacca gcaaaagtgc
    35881 gaataccact tgcccgaaag gcccgtcgcg agtactttgt cgcgatattt ttgacatttt
    35941 cgactacagg aatttttcga tgccaagagc gaacgaaatt aaaaaaggta tggtactgaa
    36001 ttacaacggc aaactgctgc tggttaagga tattgatatt cagtcgccca ctgcccgcgg
    36061 tgccgctacg ctgtacaaaa tgcgtttttc tgatgtccgt accgggctga aagtagaaga
    36121 gcgtttcaaa ggtgatgata tcgttgacac cgtgacgctg acccgccgtt acgttgactt
    36181 ctcctatgtc gatggcaacg aatatgtctt tatggataaa gaagactata ccccgtatac
    36241 cttcaccaaa gatcagattg aagaagagtt gctgtttatg ccagagggcg gcatgccgga
    36301 catacaggtg ctgacctggg acggtcaact gctggcgctt gagctgccgc agaccgttga
    36361 tctggaaatc gttgaaaccg caccaggcat caaaggggca tctgccagcg cccgtaacaa
    36421 accggcgaca ttgagcactg gtctggtgat tcaggtacca gaatacttaa gcccgggcga
    36481 aaaaattcgt atccatatcg aagaacgccg ttatatgggg cgtgctgact aacttcagcc
    36541 gcatgcagaa aagggatagc tcaggctgtc ccttttttaa tttattatac caatcttcta
    36601 ttttgcgctt cctgaacacc ttacgccctc aattttcact cgttgattga tcgccctcac
    36661 acttcatcgc attaacaatc cagaccaatt tcaattgctg tcatataact ttacactgtc
    36721 attgttaatt tatcgttact aagacgtgac tcctatgaag acaattgcct ccgttacgct
    36781 cccgcatcat gtacacgctc cacgctacga tcgccagcag ttgcaatcac gtatcgttca
    36841 ttttggcttt ggcgcctttc accgcgctca tcaggcgtta ctgaccgatc gtgtgctgaa
    36901 tgcccagggc ggcgactggg ggatctgtga aatcagcttg ttcagcggtg atcaactgat
    36961 gagccagctc cgcgcacaga accatttata taccgtgctg gagaaaggtg cggacggcaa
    37021 tcaggtgata attgtcggtg ccgttcacga atgccttaat gcaaaactgg attccttagc
    37081 ggcaattatt gagaaatttt gcgagccaca ggtggcaatt gtttccctga cgattaccga
    37141 aaaaggctat tgtattgacc cggccaccgg tgcactcgac accagtaatc cgcggattat
    37201 tcacgatcta caaacccctg aagaacctca ctccgcaccg ggtattctcg tcgaagcact
    37261 gaaacgccgc cgtgagcgcg gccttacacc gtttaccgtg ctctcctgcg acaatattcc
    37321 cgacaatggt catgtggtga aaaacgcggt gctgggaatg gcagaaaaac gttcgccaga
    37381 actcgccggg tggataaaag agcacgtcag ttttccggga accatggtcg accgcattgt
    37441 tccggctgca accgacgaat cactggtgga aatcagccag catctggggg tgaatgatcc
    37501 ctgcgcgatt agctgcgaac cgtttatcca gtgggtggtg gaagataact tcgtcgctgg
    37561 gcgtcctgcc tgggaagtcg caggtgtaca aatggtgaat gatgtcctgc catgggaaga
    37621 gatgaaactg cggatgctta atggcagcca ctcttttctc gcttatctgg gttacctctc
    37681 aggattcgcc catatcagtg attgcatgca ggatcgcgca tttcgccatg ccgccagaac
    37741 attaatgctg gatgagcaag cgccgacact gcaaattaaa gatgtcgatt taacacaata
    37801 tgcggataag ttaattgcac gttttgctaa tccggcgctg aaacataaga cctggcaaat
    37861 cgcgatggat ggcagccaga aattaccgca acgcatgctg gcaggtattc gcatacatca
    37921 ggggcgcgaa acggactggt cgttgctggc attaggcgtt gcaggctgga tgcgttacgt
    37981 cagcggcgtt gatgatgccg gaaatgccat tgatgttcgc gatccgctta gcgataaaat
    38041 tcgcgaactt gttgcgggca gcagcagtga acaacgcgta accgccctgc tttccctgcg
    38101 tgaagttttc ggtgatgatc tgccagataa cccgcatttt gtgcaggcca tcgaacaagc
    38161 ctggcaacaa atcgtacaat tcggcgcaca tcaggcgcta ttaaacaccc tcaaaattta
    38221 acgatttctg cggttaaagc ggatgaagct caccttcgtc cgctctcccc ttctcttttc
    38281 tgcttttttt agccaggatt aacgctcagt taacttacca gagtattgcg gagccgttgt
    38341 gaccaggacc aacctcatca ccggttttct cggcagcggg aaaaccacgt cgattcttca
    38401 tctgttagcc cataaagatc ccaacgaaaa atgggcggta ctggttaatg aatttgggga
    38461 agtcggaatt gatggtgctt tgctcgccga tagcggcgca ttgctgaaag agatccccgg
    38521 cggctgcatg tgctgcgtta atggtttacc catgcaggta gggttgaata ccttactgcg
    38581 tcagggaaaa ccagaccgct tgttgataga gccgaccggg ctgggccatc cgaaacagat
    38641 cctcgatctg ttaaccgcac cagtctatga accgtggata gatctgcgcg ccaccttgtg
    38701 cattctcgat ccgcgcttgc tgctggacga aaaaagcgcc agcaatgaaa acttccgtga
    38761 ccagctggct gccgcagaca tcattgtcgc caataaatcc gaccgtgcga cgcccgaaag
    38821 tgagcaagcg ctacagcgtt ggtggcagca aaatggtggc gatcgacaat taattcacag
    38881 tgagcatggg aaagttgacg gtcatcttct ggatttgccg cgtcgcaatt tagccgagtt
    38941 gcccgccagc gccgcgcatt ctcatcagca tgtcgtgaaa aaagggttag cagcgttaag
    39001 cctgccagag catcaacgct ggcgtcgcag tctgaacagc gggcaaggat atcaggcctg
    39061 cggctggata ttcgacgctg atacggtatt cgacaccatt ggcattctgg aatgggcgcg
    39121 acttgcaccg gtggaacgcg tcaaaggcgt gctgcgtatt cccgaagggc tggtacgaat
    39181 caaccgtcag ggcgatgacc tgcacattga aacgcaaaac gttgcgccac cggacagccg
    39241 tattgagctg atttccagca gcgaagctga ctggaatgct ttgcagagcg cgctgttgaa
    39301 gcttcgttta gcgactaccg cgtaaggttg cctgcgtttt tcagtaagat aattagagaa
    39361 aatatgatta aaaatttgcc gcaaatagtg ttgttgaata ttgtcggcct cgcgctgttt
    39421 ctttcctggt atattcccgt taatcatgga ttctggttgc cgattgatgc ggatattttt
    39481 tatttcttta atcagaaact ggtcgaaagt aaggcctttt tgtggctggt tgcattgacc
    39541 aacaatcgcg ctttcgacgg ttgttctctg ctggcgatgg gaatgttgat gctgagtttc
    39601 tggctgaaag aaaacgcccc tggcagacga cgtatcgtga ttatgggtct ggtcatgcta
    39661 ttaactgcag tggtattaaa ccagctgggt caggcattaa ttcctgtaaa acgggccagc
    39721 ccaacattga cttttaccga tattaaccgc gtcagcgaac tgctctctgt tcccacgaaa
    39781 gatgcctcac gagatagctt tcccggcgat cacggcatga tgctgcttat tttttcggca
    39841 ttcatgtggc gttatttcgg caaagttgca ggccttatcg cccttattat ttttgtggtt
    39901 tttgcatttc ccagagtaat gattggcgca cactggttta ctgacatcat tgtcggttcg
    39961 atgaccgtga tattgatcgg tttgccctgg gtgttgctga cgccattaag tgatcgatta
    40021 atcacctttt ttgacaaatc actaccggga aaaaacaaac atttccaaaa caaataactc
    40081 acagtaatta acatcatcag gcttattttt atagtgagga taatcctgat gatgcgcacc
    40141 gtgctttcat ccatcgcgcg caaaaatcat tctctaagta aatgaatgga ttgcatgcgt
    40201 ttcactcaat cgtactttaa ttgaccaatc ccgcttatta actttctgta tcactttttc
    40261 ttataaaaaa tcatgtaaaa ccgctcgcca agaccgcacc aatcgggtaa tctcgaactc
    40321 gttttgcctc ggcggtagat tatcctcaca gcatataatt ttgtgcgtta gtccacagat
    40381 ttggccttaa ggaattgttt caacatgccc aggtaattag tctcgtgtcg cttggcattt
    40441 ttttataacg atatttgtcg ttaaggactt caagggaaaa caaacaacat ggtcaaatct
    40501 caaccgattt tgagatatat cttgcgcggg atccccgcga ttgcagtagc ggttctgctt
    40561 tctgcatgta gtgcaaataa caccgcaaag aatatgcatc ctgagacacg tgcagtgggt
    40621 agtgaaacat catcactgca agcttctcag gatgaatttg aaaacctggt tcgtaatgtc
    40681 gacgtaaaat cgcgaattat ggatcagtat gctgactgga aaggcgtacg ttatcgtctg
    40741 ggcggcagca ctaaaaaagg tatcgattgt tctggttttg tacaacgtac attccgtgag
    40801 caatttggct tagaacttcc gcgttcgact tacgaacagc aggaaatggg taaatctgtt
    40861 tcccgcagta atttgcgtac gggtgattta gttctgttcc gtgccggttc aacgggacgc
    40921 catgtcggta tttatatcgg caacaatcag tttgtccatg cttccaccag cagtggtgtt
    40981 attatttcca gcatgaatga accgtactgg aagaagcgtt acaacgaagc acgccgggtt
    41041 ctcagccgca gctaataaac cgtttggatg caatcccttg gctatcctga cgagttaact
    41101 gaaagcactg cttaggcagt gcttttttgt tttcattcat cagagaaaat gatgtttccg
    41161 cgtcttgatc caggctatag tccggtcatt gttatctttt aaatgttgtc gtaatttcag
    41221 gaaattaacg gaatcatgtt catacgcgct cccaattctg gacgtaagct cctgcttacc
    41281 tgcattgttg caggcgtgat gattgcgata ctggtgagtt gccttcaatt tttagtggcc
    41341 tggcataagc acgaagtcaa atacgacaca ctgattatcg acgtacaaaa gtatctcgat
    41401 acctattttg ccgacctgaa atccactact gaccggcttc agccgctgac cttagatacc
    41461 tgccagcagg ctaaccccga actgaccgct cgcgcggcgt ttagcatgaa tgtccgtacg
    41521 tttgtgctgg tgaaagataa aaaaacattc tgttcatctg cgactggtga gatggacatt
    41581 ccactcaatg aattgattcc ggcgctcgac attaataaaa acgtcgatat ggcgatctta
    41641 cccggcacgc cgatggtgcc gaacaaaccc gcaatcgtca tctggtatcg caaccctttg
    41701 ctgaaaaata gcggtgtgtt tgccgctctg aatctcaacc tgacgccttc tctcttttat
    41761 agttcacggc aggaagatta cgatggcgtc gccctcatta ttggcaatac tgcgctatct
    41821 accttttctt cacgtttgat gaacgttaat gaattaaccg acatgccggt ccgtgaaact
    41881 aaaattgcgg gcattcctct gaccgttcgg ctttatgcgg atgactggac atggaacgat
    41941 gtgtggtacg catttttact gggcggcatg agtggaactg tcgttggcct gctctgctat
    42001 tacctgatga gcgtacgtat gcgccccggc agagaaatca tgaccgccat caagcgcgaa
    42061 caattttacg tggcgtatca accggtggtg gatacacaag ctttgcgagt aacgggcctg
    42121 gaagtactgc tacgctggcg gcatcctgtc gcgggagaaa ttcccccgga tgccttcatt
    42181 aactttgccg aatcgcaaaa gatgattgtg ccgctgactc agcacctgtt tgagttaatt
    42241 gcccgcgatg ccgcagaatt agaaaaagtg ctgccagtag gcgtcaaatt tggtattaac
    42301 attgcgccgg accatctgca cagcgaaagc tttaaagcag atatccagaa actgctcact
    42361 tccctacccg cacaccattt ccagattgtg ctggaaatta ccgagcgcga tatgctgaaa
    42421 gagcaagaag ccacacaact cttcgcctgg ctgcactcgg tcggcgtaga aattgctatt
    42481 gatgacttcg gcaccgggca cagcgcgctt atctatcttg agcgttttac gctcgattat
    42541 ctgaaaattg accgtggatt tatcaacgcc atcggtacgg aaacgatcac ttcacccgta
    42601 cttgacgcgg tgctgacgct ggcgaaacgt ctcaatatgc tgacagttgc tgaaggggtc
    42661 gaaacgccgg aacaagcgcg atggctaagc gaacgcggcg ttaatttcat gcaaggctac
    42721 tggattagtc gcccgttacc gctggacgat tttgtccgct ggctgaagaa accgtatacg
    42781 ccgcagtggt aaggtgtgct tacgtccctt attattcata gtgaaagcat gccggattgc
    42841 ggctaatgat gagtaaaagg aaatccgttg cagatgattg tgcgcatact gctgctgttt
    42901 atcgctctgt tcacctttgg tgtgcaggcg caggctatca aggaaagcta tgcctttgcc
    42961 gtgctgggcg aaccccggta cgcgtttaat ttcaaccatt ttgattatgt gaaccccgcc
    43021 gcgccaaaag gtgggcagat aacgttgtca gccctcggca ccttcgatac tttcaaccgc
    43081 tatgcactgc gcggcaaccc gggcgcacgc accgagcagc tgtacgacac gctatttacg
    43141 acttccgatg acgaaccagg cagttattac ccgctgattg ctgaaagcgc acgctatgct
    43201 gacgattatt cctgggtgga ggtcgctatt aatccgcgcg cccgttttca tgatggttcg
    43261 cccattactg cccgcgatgt agagtttact tttcaaaaat ttatgaccga aggcgtgccg
    43321 caatttcgtc tggtctacaa aggcaccacc gtcaaagcca ttgcgccgtt aaccgtgcgc
    43381 attgagttag ctaaacccgg caaagaagat atgttgagtc tgttttcgct gccggtattt
    43441 ccagaaaagt actggaagga tcacaaactt agcgacccgc tcgccacgcc tccgcttgcc
    43501 agtggtccgt accgcattac gtcctggaaa atggggcaaa atattgtcta ttcccgcgtg
    43561 aaagattact gggcagcaaa cttaccggta aaccgcggac gctggaattt cgacaccatt
    43621 cgctacgatt attacctcga tgataatgtc gcctttgaag cgtttaaagc aggtgccttt
    43681 gatttgcgta tggaaaacga cgccaaaaac tgggccacgc gttataccgg taaaaatttc
    43741 gataaaaaat acatcatcaa agatgagcaa aagaacgaat cagcccagga tacgcgctgg
    43801 ctggcgttta atatccaacg tccagtattc agcgatcgcc gggtgcggga agcaatcact
    43861 ctcgcctttg actttgaatg gatgaacaag gcgttgtttt acaatgcctg gagtcgcacg
    43921 aacagttatt ttcagaatac cgaatacgcg gccagaaatt accccgacgc cgcggagctg
    43981 gtgcttctgg caccaatgaa aaaagatcta ccgccagaag tcttcacaca aatctaccag
    44041 ccgccggtat ccaaaggcga tggctacgat cgtgacaacc tgttaaaagc cgacaaactt
    44101 ctcaacgaag cgggctgggt gctgaagggt cagcaacgcg ttaatgccac aacgggtcag
    44161 ccactcagct ttgaattatt gcttcccgca agcagcaata gtcagtgggt attgccgttc
    44221 cagcacagcc tgcaacggct gggtatcaac atggacattc gcaaggtgga taactcgcaa
    44281 atcactaacc gcatgcgcag tcgcgactat gacatgatgc cgcgcgtatg gcgggcgatg
    44341 ccgtggccca gttccgattt acagatttcc tggtcatcgg aatatatcaa ttccacttat
    44401 aatgcccccg gcgtgcaaag cccggttatc gactcgctga tcaaccaaat tattgccgcg
    44461 cagggaaata aagaaaaatt actgccgttg gggcgagcac tggatcgcgt attaacgtgg
    44521 aattattaca tgctgccaat gtggtacatg gcggaagacc gtctcgcctg gtgggataaa
    44581 ttctcccagc cggccgtgcg ccccatctat agcctcggta tcgatacctg gtggtatgac
    44641 gtcaataaag cggccaaact gccgtccgcc agcaaacagg gagagtagat gggcgcttac
    44701 ctgattcgcc gtctgttgct ggtgatccca acattatggg cgattatcac catcaacttt
    44761 ttcatcgtgc aaattgcgcc tggcggtccg gtcgaccagg ccatcgccgc cattgagttt
    44821 ggtaatgccg gagtattacc cggcgcaggc ggtgaaggtg ttcgtgccag ccatgcgcaa
    44881 acgggtgtcg gcaatatcag cgacagtaag taccgtggcg gacgcggatt agatccagaa
    44941 gtgatcgctg agatcactca tcgctacggt tttgataagc cgatccacga acgttacttc
    45001 aaaatgctct gggactacat ccgctttgat tttggcgata gcctgtttcg cagcgcctcg
    45061 gtgctgacgc tgattaaaga cagtctgccg gtttccatca ccctcggatt gtggagcacg
    45121 ctgattatct atctggtgtc gattccgtta ggcattcgca aagctgttta taatgggagc
    45181 cgctttgacg tctggagtag cgcatttatc atcatcggct acgccattcc ggcctttttg
    45241 tttgccatcc tgctgattgt cttcttcgcg ggcggcagct atttcgacct gttccctcta
    45301 cgcggcctgg tttccgctaa ctttgattcg ctgccgtggt atcagaaaat caccgattat
    45361 ctgtggcata tcacgctgcc ggtgctggcg acagtgattg gtggctttgc ggcgctgacc
    45421 atgctgacaa aaaactcatt ccttgatgaa gtgcgcaagc aatacgtggt gaccgcgcgt
    45481 gcgaaagggg taagtgaaaa aaatattctc tggaaacatg tgttccgcaa cgccatgctg
    45541 ctggtgattg ccggttttcc ggcgacgttt atcagcatgt tttttaccgg ctcgctgctg
    45601 attgaggtga tgttttcact caatggtctg ggcttactgg gctacgaagc gaccgtctcg
    45661 cgcgattatc ctgtaatgtt tggtaccttg tatattttca ccctgattgg cctgctgctg
    45721 aatattgtca gtgatatcag ctatacgctg gtcgatccgc gtatagattt tgagggacgt
    45781 taatgtcgcg actcagcccc gtcaatcagg cccgttgggc gcgttttcgc cataaccgtc
    45841 gcggctactg gtcgttatgg attttcctcg ttttgtttgg tttgagtttg tgttctgaac
    45901 ttatcgccaa cgataaaccg ttgctggtac gttatgacgg cagttggtat ttcccgttgt
    45961 tgaaaaacta cagcgaaagc gattttggcg gcccgctggc aagtcaggct gattatcagg
    46021 acccgtggct gaaacaacgg ctggaaaata acggctgggt actgtgggca ccgattcgct
    46081 ttggtgctac cagtatcaac tttgctaccg ataagccctt cccttctcca ccctcccggc
    46141 aaaactggct gggaacggat gccaacggcg gcgatgtgct ggcacgtatt ctctatggca
    46201 cgcggatctc ggttctgttt ggcctgatgc tgactctctg ttccagcgtg atgggcgtgc
    46261 tggcgggggc gctacaaggc tattacggcg gtaaagtcga tctctggggg caacgcttta
    46321 ttgaagtatg gtcggggatg ccgacgctgt ttttgattat tttactttcc agcgtcgtac
    46381 agcctaactt ctggtggctg ctggcaatta ctgtcttgtt tggctggatg agtctggtcg
    46441 gcgtggtgcg ggcggagttt ttacgcaccc gtaatttcga ctacattcgt gcggcgcagg
    46501 cgcttggcgt cagcgatcgc agtatcatcc tgcgtcatat gttgcctaat gccatggtcg
    46561 cgaccctcac ctttttaccg tttattttat gtagttcgat aaccaccctg acctcactcg
    46621 atttcctcgg cttcggtctg ccgctcggtt caccgtcact cggtgaactg ctgttacaag
    46681 ggaaaaataa ccttcaggct ccgtggcttg ggatcaccgc cttcttgtcg gtggcgatat
    46741 tattgtcttt gctgatcttt attggtgaag ccgtccgcga cgcatttgat cctaataagg
    46801 cggtgtagca tgacgcaaac tctgttagcg attgaagatt tgtcggtggg ttttcgccat
    46861 cagcaaaccg tacgtacagt agtcaatgat gtttcactac agattgaggc tggcgaaacg
    46921 ctggcgctgg tgggtgagtc aggttcaggc aaaagcgtta ccgcgctgtc aattttacgc
    46981 ctgctccctt ccccgccggt tgaatatctc tccggcgata ttcgttttcg tggcgaatcg
    47041 ctgcttcatg ccagcgatca aacgttgcgc ggtgtacgcg gtaataagat cgccatgatt
    47101 tttcaagagc cgatggtgtc gttaaatcca ttgcataccc tggaaaaaca gctttatgaa
    47161 gtgctttcac tccaccgcgg gatgcgtcgg gaagcggctc gaggcgaaat tcttaactgc
    47221 cttgatcgcg ttggtatccg ccaggcggca aaacggctga cagattatcc gcatcagctc
    47281 tccggcggcg aacgccagcg ggtgatgatt gcgatggcgc tgttaacgcg accggaatta
    47341 ttaattgccg atgaaccgac caccgcactg gacgtctctg tccaggcgca gattttacag
    47401 ctgttgcgcg aactgcaagg cgagctgaat atgggcatgc tgtttattac tcataacctc
    47461 agcattgtca gaaaactggc ccaccgcgtg gcggtaatgc aaaacggtcg ctgtgtcgag
    47521 caaaataacg ccgctacgct atttgcctca cccactcatc cttacacaca aaagctactc
    47581 aacagtgaac cgtcaggcga cccggtgccg ttgccagaac ctgcctcaac gttgctggat
    47641 gttgaacagc ttcaggttgc cttccccatt cgcaaaggga ttttgaagcg cattgtggat
    47701 cataatgtgg tggtgaaaaa catcagtttt acgctacgag cgggtgaaac actgggttta
    47761 gtgggcgagt ccggttccgg gaaaagtacg acgggactgg cgctgctgcg actgattaat
    47821 tctcagggca gcatcatctt tgacggtcag ccactgcaaa atttaaatcg ccgccagctg
    47881 ttacctattc gtcatcgcat tcaggtggta tttcaggatc caaactcctc gctcaaccca
    47941 cgactcaacg ttttgcagat tattgaggaa ggcttacggg ttcaccagcc gacgctttct
    48001 gccgcacaac gcgaacaaca agtgatagcc gtgatgcatg aagtgggatt agatcctgaa
    48061 acacgccacc gttatccggc ggagttctct ggtggtcagc gacaacgtat tgcgattgcc
    48121 agggcattaa ttcttaagcc ctcgctgatc atacttgatg aaccgacatc atcactcgac
    48181 aaaacggtac aggcgcaaat attgacgcta ttgaaatcat tgcaacaaaa gcatcaactg
    48241 gcctatttgt ttatcagcca cgatttgcac gttgtccgcg cgttatgtca tcaggttatc
    48301 gtactgcgac aaggggaagt agtggaacaa ggaccgtgcg cgcgcgtgtt tgccacaccg
    48361 cagcaggagt atacgcgtca gctactggcg ttgagctgac gcttaaaaag gattgtagtt
    48421 tgaaaagggt tcggcaattg ccacaccaaa atttttcaat cggcatgttg cagcaaactc
    48481 gtcctggcga tttacaaaca ggcacggctc accttcacac tccagcaccg aacatggaac
    48541 ttcgatgtcg cttaacgcct ggcttagttt atacatcacc gaccatgcat tatcaccatc
    48601 cgggctaagc aatttaagag ccaccaggct gttgtcaccg cacggtccat tttgcggaat
    48661 cggttcaacc ttcgaacctg cgaaaccagc agaccagcga tagttctgcg gcagtcgatg
    48721 gacgattgag agttgtaatg aagtcactta ttttccccgg aagcacattc acttcacaat
    48781 ttgtttacat caattttaac acatcatcaa caatccgtct ttatacagat cgtaaagatt
    48841 gatgcctcgc ctgcgtggcc tctatggctc tgatttaagt aatatctgcg ggcgtggtca
    48901 cgtttgcggg gctatatgta cccgtttctg cgatctaact caaccttttt aactacaatg
    48961 atgtgacttt ttacataaat tgattttaca taaaataaac atatatcggg ggaatgatag
    49021 atttgtggtt gacatgcatc aacaaaggaa gccttttagc ttcctcgttg tgcaatagat
    49081 caccgttttt tcggtcgact ggcgtacaga cagaagagaa tggagctgct tgcgcagaat
    49141 gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg caagagaaag caatgcgcca
    49201 acaattgccc ctatgccaaa acggaaggtt cctgccagcg aagatgccgt tcccgccata
    49261 tggggaaact catcaagaat gaccgccatc gcattggatg acaccatcga cacgcagccc
    49321 acaaacgccg caacgccaac caccagcgac cagaatccca gccccagcag cgcactgatg
    49381 accatccacg ctgccataat aaattgtatc cacaaccccg agcggaacat atttaacgcg
    49441 ccaatgcggc gaacgaagcg gctgttaaaa atggtcatca cgaacaggaa aacaatgttt
    49501 agtgcaaagt aataaccaaa gttttccggc gcgacgtggt taatttcaat gtagacaaac
    49561 ggtccggcgc ttaagaacga gaacatcccg gcaaagctga aaccactggc aagcatataa
    49621 ctcaggacac gcttatgacg gaacagcgcc gcaaagttac caatagtggt acgaatgtga
    49681 aaaggctggc gacgctccgg tggtaaggtt tctttaatca ggaagaaaat cattgccgaa
    49741 gccagaatcg ccgctaatgc caggatccag aagatgtaat gccagctcag ccacaccagc
    49801 acccaaccgc cgacaatcgg tgccatcagc ggtgcaatag tggtcaccag catgacaaac
    49861 gacatcatcc gcgagaactc ttctttcggg taaatatcgc gcatcagggc gttaatgacc
    49921 acgctggccg cagccgccgc cagcccgtgg aagaaacgca tcacaatcag ctgatcgatg
    49981 gtgtttgcca acgcacacgc caccgcggcg gcggcaaaca ccagcgtacc gccgagcacc
    50041 accggcttac gcccgaagct gtctgccatc ggcccgtaga ttaactgccc caacgcaaag
    50101 cccagaatat aagtactgag ggtcatctgc gtactgcccg caggtacgcc aaactgcgct
    50161 gaaattaccg gtagcgcggg cagatacata tcaatcgaca gcggcatcaa catggccagc
    50221 aggccaagga taaaaacaat agcaaacgac gaatgctgtc gggtggtcac aacgggctcc
    50281 tgaaagtcat ggaaaagtta gacgacgctg gcaatttctt cttcagttaa cggacgatat
    50341 tcaccggggg ctaaatcagc atccagcgta ataccgccaa tacgttcacg atgcagctca
    50401 accacgtggt tacccacggc ggcgaacatg cgtttcacct gatgataacg cccttcgctg
    50461 atggtcagac gaacctgcgt tggggtaatc acttccagca ccgcaggctt agtgagatct
    50521 ttttcgttat gcagctgcac gcctttagca aattgctctg ccgtatcgtc agctacaggt
    50581 gattccagtg tcaccagata ggtcttctcg caatgatggc gcggagaagt aatgcggtgc
    50641 gaccactgac catcatcagt catcagcacc agaccggtgg tatcgatatc cagacgtcct
    50701 gcggcgtgca gtttccaggc taccggttca tccagaaaat agagcaccgt tgggtgatca
    50761 gggtcgtccg tggagcaaac atagccctga ggcttattga gcatgaagta acgtggacca
    50821 tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat gttcaggaag cagtttgaac
    50881 gctgcattac gaacaatttc gccatcgacg gtgacacgat tgccgcggat ttcacgcccg
    50941 gcaatagcac ggctaacgcc gagttgctgt gcgataaatt tatcaagtcg catgtgtgtg
    51001 attttgcctg taaaaacgga ggtcgggcat tgacccgaaa atctgaactg ttgtctgccc
    51061 agtatagcgg tctaataaca tccctcaagg gaaaaagatc catggcatac tattagcaga
    51121 ataatctacc tacgcgagac catgattttt acactccgcc catatcagca agaagccgtg
    51181 gatgccacgc tcaaccattt tcgtcgtcat aaaacccctg ccgttatcgt gctgcccacc
    51241 ggcgcaggta aaagcctggt gattgccgaa ctggcacggc tggcgcgtgg tcgtgttctg
    51301 gtgctggcac acgtcaaaga actggtggcg caaaaccatg caaagtatca ggcgctgggg
    51361 ctggaagccg atatttttgc cgctgggtta aagcgcaaag agagccacgg taaagtggta
    51421 tttggcagcg tgcagtcggt cgctcgtaat cttgatgcct ttcagggtga attttcgctg
    51481 ttgattgtcg atgaatgcca ccgtattggt gacgatgaag agagccagta tcagcaaatc
    51541 ctcactcacc tgactaaagt gaatccccac ttacgcctgc tggggctgac tgccacgcct
    51601 tttcgattgg gcaaaggctg gatctaccag ttccattatc acggcatggt acgcggcaat
    51661 gagaaagccc ttttccgtga ctgcatttat gagctgccgc tgcgttatat gattaaacac
    51721 ggctatctga cgccgccaga acgactggat atgccagtag tgcaatacga tttcagccgc
    51781 ttgcaggcac agagtaacgg gctgttcagc gaagctgatc tcaaccgtga gctgaaaaaa
    51841 cagcaacgca ttacgccaca tatcatcagc cagatcatgg agttcgccga aaagcgcaaa
    51901 ggggtaatga ttttcgccgc caccgttgaa cacgcaaaag agattgtggg attactgcct
    51961 gccgaagatg ctgcactgat tactggcgac acccccggcg ctgagcgcga tgtgttaatt
    52021 gaagatttta aagcccagcg ttttcgctat ctggtcaacg tcgcagtact gaccaccgga
    52081 tttgacgccc cgcacgtcga tcttatcgcc attctgcgcc ctaccgaatc agtgagtctt
    52141 taccaacaaa ttgtcgggcg cggtctgcgt ctcgctccgg gcaagactga ttgtttaatt
    52201 cttgattatg cgggtaatcc tcacgatctc tacgcgccgg aagttggtac accgaaaggc
    52261 aaaagtgaca acgttccggt acaggttttc tgccctgcct gcggttttgc caacaccttt
    52321 tgggggaaaa cgaccgccga cgggacattg attgaacact ttggtcgccg ctgtcaggga
    52381 tggtttgaag atgacgacgg tcatcgcgaa cagtgtgact tccgtttccg ttttaaaaat
    52441 tgcccgcaat gtaacgcaga aaatgatatt gccgctcgcc gctgccgcga atgtgacacg
    52501 gtactggttg atccggatga tatgttaaaa gcggcgctac gactgaaaga tgcgctggta
    52561 ttacgctgta gcggcatgtc tttgcagcat gggcacgacg agaaaggcga atggttgaaa
    52621 atcacctatt acgatgaaga cggcgcggat gtgagtgagc gtttccgtct gcaaacgccc
    52681 gcccagcgaa ctgccttcga gcagcttttt atccgcccgc atacgcgcac accgggcatc
    52741 cctctgcgct ggatcaccgc cgccgatatc ctcgcccagc aagccttact gcgacatccg
    52801 gattttgtcg tcgcccgcat gaaaggtcag tactggcagg tgcgtgaaaa agtgttcgat
    52861 tacgaaggtc gttttcgtcg ggcgcacgaa ttacgcggtt aatggtagtt ttgattgatg
    52921 tataaggcga ttgagtatag aatctcgccc gcttttgcat acgcaaagca gatcacttac
    52981 ctgttgctgg gtcgcctgta gcaggattaa tttaagagag aaagaaatgt ttactatcaa
    53041 cgcagaagta cgtaaagagc agggtaaggg tgcgagccgc cgcctgcgtg ccgctaacaa
    53101 gttcccggca atcatctacg gtggcaaaga agcaccgctg gctatcgagc tggatcacga
    53161 caaagtcatg aacatgcaag ctaaagctga attctacagc gaagttctga ccatcgttgt
    53221 tgacggtaaa gaaatcaaag ttaaagctca ggacgtacag cgtcacccgt acaaaccgaa
    53281 gctgcagcac atcgacttcg ttcgcgctta attgctgaat aagttgtaaa aaaccccgct
    53341 ccggcggggt tttttgtatc tgcagattat gcctgattac ggtattgcta ttttttgcag
    53401 gccagataag gcgttcacgc cgcatctggc attcagaaca caaaaccgat taattgccgc
    53461 cagacgtccg gcgctgcaat tggtcgcgca aattcggcgg cgtgccttta atggtcaagg
    53521 tatcggttgc tggatcccag aagatgcgtt cacctaacag catcgcatca aagttaatag
    53581 tcaagccacc gccgctaccg gcaaacttcg tcaactgacg cagtgtgcta cggtctgccg
    53641 ggaagctctc ttccagttcg tagccttttt cagccgcaaa ctccgtgaag ctcacttcgc
    53701 ttacgcctgc cagctctttc gacagcgatt ccagttcgat ctcttcgcct gcctgcagct
    53761 gctcattaca gtagctataa acctgctggc gcacgttctg tcgctctgct ttatccagtt
    53821 gtgcttcggc agtgaaatca tcaaccgctt gcaacagccc acggttttgc gctttcgcgt
    53881 tcaatccttc gctggccccg aggaaatcca taaagaaatc cgccactttg cgccctactc
    53941 gccctttcag gaaagtgaga tagcgggtgg actctggatt ggtttcccat tcggttaaat
    54001 cgatacgcgc aacaatatcc gcatgattga tatcaagata atgggttggg ttgatgtcga
    54061 gattttcgtt aacgcgcata ctgctcaggt tgctcagaac tgccaccagc agatactcca
    54121 ccgccagata acggtagtgg caaaacagca cgaatccacc gtcagcaaaa ggatatttcg
    54181 ctaattcgtc acgcaggcgc ccggttgccg cgcggctaaa tgccagaaag tcctcttcgc
    54241 cctgacgctg taagcgcagc gtctgcgcca gttcactctc ttcgctaaac aagccgtaag
    54301 ctttattttt ggcgctatag acccgatgca gttctgccac catctcaacg acggtttctg
    54361 tcggttccag caatgaatcg cgcaacacca gctcaaggtt ctgctcatca cgcttgataa
    54421 gctggtgcag ggcaatctgg ttgatatcca gactcatgat aaactctcct ttaagaccgg
    54481 gcggtattca accaccgccg gttaagcgac gcaataaaag atggcggtca ttttacagaa
    54541 ggcagcgtaa cgagaatgat ttaacgcata aaaaagcaga aaaaaaaccg ttgctacggt
    54601 aatatgttgc cctttcatca actaactgat ttcgatttat gccacaaatt tcccgctaca
    54661 gtgatgaaca ggttgaacaa ttgctcgcag agctgctcaa cgtactggaa aaacataagg
    54721 ctccgaccga cctttccctg atggtgttag gcaacatggt cactaacctt atcaacacca
    54781 gcattgcccc ggcccaacgc caggcaattg ccaactcttt tgcccgcgcc ttacagtcct
    54841 ctatcaacga agacaaagcg cactaaggga aacagataac tggttatggt aactcatcgt
    54901 cagcgctacc gtgaaaaagt ctcccagatg gtcagttggg ggcactggtt tgcactgttc
    54961 aatattctgc tttcgctcgt cattggcagc cgttacctgt ttatcgccga ctggccgaca
    55021 acgcttgctg gtcgcattta ttcctacgta agcattatcg gccatttcag cttcctggtg
    55081 ttcgccacct acttgctgat cctcttcccg ctgaccttta tcgtcggctc ccagaggctg
    55141 atgaggtttt tgtccgtcat tctggcaacg gcgggaatga cgctattact gatcgatagc
    55201 gaagtcttta ctcgtttcca tctccatctt aatcccatcg tctggcaact ggttatcaac
    55261 ccagacgaaa atgagatggc gcgcgactgg cagctgatgt tcatcagcgt gccggttatt
    55321 ttattgcttg aactggtgtt tgcgacgtgg agctggcaaa agctgcgcag cctgacgcgt
    55381 cgtcgacgct tcgcgcgccc gctggccgca ttcttattta tcgcctttat cgcctcgcat
    55441 gtggtgtata tctgggccga tgccaacttc tatcgcccga tcaccatgca gcgcgctaac
    55501 ctgccgcttt cgtacccgat gacggcgcga cgttttcttg agaagcatgg tctgcttgat
    55561 gcgcaggagt atcaacgccg tcttattgag caaggtaatc cagacgccgt ttccgtacag
    55621 tatccgttaa gcgaactgcg ctatcgcgat atgggcaccg ggcagaatgt gctgttgatt
    55681 actgtcgatg gcctgaacta ctcacgcttc gagaagcaga tgcctgcgct ggcaggtttt
    55741 gctgagcaaa atatttcgtt cacgcgccat atgagctccg gcaacactac agacaacggc
    55801 atctttggcc tgttctatgg catctcgccg agctatatgg acggcattct gtcgacccgt
    55861 acgcctgcgg cattaattac tgcgcttaat cagcaaggct atcagctggg gttattctcg
    55921 tcagatggct ttaccagccc gctgtatcgc caggcattgt tgtcagattt ctcgatgccg
    55981 agcgtacgca cccaatccga cgagcagacc gccacgcagt ggatcaactg gctgggccgc
    56041 tacgcacaag aagataaccg ctggttctcg tgggtctctt tcaatggcac taacattgac
    56101 gacagcaatc agcaggcatt tgcacggaaa tatagccggg cggcaggcaa tgtcgatgac
    56161 cagatcaacc gcgtgctcaa tgcactgcgt gattctggca aactggacaa tacggttgtg
    56221 attatcactg ccggtcgggg tattccgctg agcgaagacg aagaaacctt tgactggtcc
    56281 cacggtcatc tgcaggtgcc attagtgatt cactggccag gcacgccggc gcagcgtatt
    56341 aatgcgctga ctgatcatac cgatctgatg acgacgctga tgcaacgcct gctacatgtc
    56401 agcacacctg ccagcgaata ttcgcaaggt caggatttgt tcaaccctca acgccgtcat
    56461 tactgggtta ctgcagcgga taacgatacg ctggcaatta ccaccccgaa aaagacgctg
    56521 gtgctgaaca ataacggtaa ataccgcact tacaacttac gtggtgaaag agtgaaagat
    56581 gaaaaaccac agttaagttt gttattgcaa gtgctgacag acgagaagcg ttttatcgct
    56641 aactgattaa ttataaatca gttagcgaaa tatcttactt gcaatcggtg tggaaaacgg
    56701 tagtattagc agccacgagt cggcacgtag cgcagcctgg tagcgcaccg tcatggggtg
    56761 tcgggggtcg gaggttcaaa tcctctcgtg ccgaccaaat cgcccaccgg atatcaccgg
    56821 aaagcaccgg ataagacagg gataacccgc taatcacttg attggcgggt ttttttatgt
    56881 tttttagtca ccgaacatca ccggatttat aagctatcag ccggattttt tagttatacg
    56941 tttagttata cgggctggcg tataactaaa gccgtataac taaaaccctg ttttttcgtg
    57001 tggaatcata gacatatggc gagaaaaaca cacccattaa ccacagtgca gatcaaagca
    57061 gccagaccag cgcaaaagga gtacaccctg caggacggcg gagggctttt tctcctggtc
    57121 aaaccgtctg gatcaaaact ctggagattt tcctactacc gaccatcgga caaaaaaaga
    57181 atattgctga gttttggatc gcttgaagat gtttccctgg ctgatgccag aaaacgccgt
    57241 agcgagtaca ggacgttaat cagtgccgga actgacccgc aggaccacga gaggcaaaaa
    57301 agagagacag aggcccgaag acaagggaac acgttcgaaa atgtggcggc ggcatggtac
    57361 caggtgaaaa tcagccagaa tctggccccc aacacgatta aagacatctg gcgttcgctg
    57421 gataaatatg tattcccgtt catcggcaac acgccaatag ataccctcac cgcccgaagg
    57481 ttcgttgaag tgcttacccc catcaaggag cgcggcaacc tggaaacact caagcgggtt
    57541 ttacagcgcg ttaatgaggt aatggattac gccgccaaca gtgggctgat tgatgccaat
    57601 ccggctatga atgtgcgtaa ggcgtttccc tcacctgtaa aaaaacatat gccaacaatc
    57661 cgccccgaac agctgcccga gcttatgcag gctttatcag tatcggcaac agaacggcag
    57721 accagattac tgattgaatg gcagttactg accgtaaccc gtcccgccga agcgtcatca
    57781 acgcggtggg atgaaatcaa cctggacgcg aagcaatgga cgatacctgc cggacgcatg
    57841 aagatgcgca gggatcacgt tatcccgctt tccggtcagg ctatggcggt gctggaggcc
    57901 atgaaaccaa tcagccacca ccgcaattac gttttcccaa gtctgaaaga cccacagcag
    57961 ccgatgaaca gccagacagc taacgcagca ttgcggcgta tgggattcgc tggcgtgctg
    58021 gtgtctcatg gattacgcgc catattcagc acagcagcga acgaggaagg attcgagccg
    58081 gacgtaatag aggcggcact tgcccacgtc gacaccaacg aagttagacg ggcatacaac
    58141 cggagcaact acatagaaaa acgcatcgtg ctgatgcgct ggtggggcga atttgtcgag
    58201 gctgcggcga cgggcgtaac cctcgccagt ggtaaaaggg gtatccgagc cgtgtagctg
    58261 tacagaaaac cagtaaaaac tacgaaaacc atgtaaaacc gtcgtataat tgcatcaaat
    58321 ttaacgacaa ggccgtgaaa catgaaaccg ttaagatgca aaaaaatatc agatgcaatt
    58381 gcgacgggct gcaactggcc ctgatggttc agcatgaatt ttggtcaacc tacgatccgg
    58441 aggacagaac gacggcccca tcaaaaaaac aggtagtaga ttttctggta tcccgtggcg
    58501 cttccagaaa tctggcggta agtattgata aggtcgtacg tccggcatct atgaagatcg
    58561 gaggcaggcc caaaaaatgg cggtaacaat cctggaagcg gcagaaatgc cgcttttttt
    58621 ataattccat ttcaaatcat caatataaaa aacggtgtat accgtttaaa aacggtggga
    58681 actgttttta cccatatccg atgatttacc gtatttgtca ccggaataca ccggattcac
    58741 aaggtaaatc acgatggaag caatcagaaa aattatcttt cgccaggaag taaaaaaaat
    58801 tatccacatc aaggcagaca gcacgctgca aagcatgatc aacgccggag aatttccgca
    58861 gggttttcgc gttggtttac gccgtcgcgg atggtatgag gatgatgtgt tgtcctggca
    58921 gaaagaacgc gaagaggaag cacgcggaac ggctgcttaa cgggtatcac agatatgaca
    58981 aacacgaaaa aagcggcccc gatatggagc cgcctttctg aacaattaac ccgctgcgcc
    59041 ttatgtgtat gtgatcccaa acataagcac ggggatgata gccgctatca ggctggtggg
    59101 caatgcaatc agtctggttc agttcgttgc catacctgca atgagcgctt ttccctgtac
    59161 tctttaagga attgctcaag ggcaaaagca catggcgcga atctttctga ttcatgctct
    59221 atctttctgc gccgtctttt ccgtgccggt gataatgttt tggtcaattc tttatcggtc
    59281 attgtgttgt cctgca
//
LOCUS       DAZGOB010000012        59237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGOB010000012 DAZGOB010000000
VERSION     DAZGOB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.012"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..81
                     /locus_tag="ACHLIF_000785"
     CDS             <1..81
                     /locus_tag="ACHLIF_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9604847.1"
                     /translation="SKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            complement(155..1357)
                     /gene="ubiI"
                     /locus_tag="ACHLIF_000786"
                     /gene_synonym="visC"
     CDS             complement(155..1357)
                     /gene="ubiI"
                     /locus_tag="ACHLIF_000786"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO9604848.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1380..2558)
                     /gene="ubiH"
                     /locus_tag="ACHLIF_000787"
                     /gene_synonym="visB"
     CDS             complement(1380..2558)
                     /gene="ubiH"
                     /locus_tag="ACHLIF_000787"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO9604849.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2555..3880)
                     /gene="pepP"
                     /locus_tag="ACHLIF_000788"
     CDS             complement(2555..3880)
                     /gene="pepP"
                     /locus_tag="ACHLIF_000788"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO9604850.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGQDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3906..4484)
                     /gene="ygfB"
                     /locus_tag="ACHLIF_000789"
     CDS             complement(3906..4484)
                     /gene="ygfB"
                     /locus_tag="ACHLIF_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO9604851.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4652..4981
                     /gene="zapA"
                     /locus_tag="ACHLIF_000790"
     CDS             4652..4981
                     /gene="zapA"
                     /locus_tag="ACHLIF_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9604852.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            5023..5206
                     /gene="ssrS"
                     /locus_tag="ACHLIF_000791"
     ncRNA           5023..5206
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHLIF_000791"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5227..5829
                     /gene="fau"
                     /locus_tag="ACHLIF_000792"
     CDS             5227..5829
                     /gene="fau"
                     /locus_tag="ACHLIF_000792"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO9604853.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5930..5989)
                     /locus_tag="ACHLIF_000793"
     CDS             complement(5930..5989)
                     /locus_tag="ACHLIF_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9604854.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6322..6381)
                     /gene="ibsC"
                     /locus_tag="ACHLIF_000794"
     CDS             complement(6322..6381)
                     /gene="ibsC"
                     /locus_tag="ACHLIF_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO9604855.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6610..7842)
                     /gene="serA"
                     /locus_tag="ACHLIF_000795"
     CDS             complement(6610..7842)
                     /gene="serA"
                     /locus_tag="ACHLIF_000795"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO9604856.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8098..8757)
                     /gene="rpiA"
                     /locus_tag="ACHLIF_000796"
     CDS             complement(8098..8757)
                     /gene="rpiA"
                     /locus_tag="ACHLIF_000796"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO9604857.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(8813..8998)
                     /locus_tag="ACHLIF_000797"
     CDS             complement(8813..8998)
                     /locus_tag="ACHLIF_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9604858.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            9140..10033
                     /gene="argP"
                     /locus_tag="ACHLIF_000798"
     CDS             9140..10033
                     /gene="argP"
                     /locus_tag="ACHLIF_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO9604859.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10237..12381
                     /gene="scpA"
                     /locus_tag="ACHLIF_000799"
     CDS             10237..12381
                     /gene="scpA"
                     /locus_tag="ACHLIF_000799"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO9604860.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            12374..13369
                     /gene="meaB"
                     /locus_tag="ACHLIF_000800"
     CDS             12374..13369
                     /gene="meaB"
                     /locus_tag="ACHLIF_000800"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO9604861.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13380..14165
                     /gene="scpB"
                     /locus_tag="ACHLIF_000801"
     CDS             13380..14165
                     /gene="scpB"
                     /locus_tag="ACHLIF_000801"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO9604862.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAQLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14189..15667
                     /gene="scpC"
                     /locus_tag="ACHLIF_000802"
     CDS             14189..15667
                     /gene="scpC"
                     /locus_tag="ACHLIF_000802"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO9604863.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15664..16560)
                     /gene="ygfI"
                     /locus_tag="ACHLIF_000803"
     CDS             complement(15664..16560)
                     /gene="ygfI"
                     /locus_tag="ACHLIF_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9604864.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16727..17467)
                     /gene="yggE"
                     /locus_tag="ACHLIF_000804"
     CDS             complement(16727..17467)
                     /gene="yggE"
                     /locus_tag="ACHLIF_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO9604865.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(17560..18195)
                     /gene="argO"
                     /locus_tag="ACHLIF_000805"
     CDS             complement(17560..18195)
                     /gene="argO"
                     /locus_tag="ACHLIF_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO9604866.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(18334..19194)
                     /gene="mscS"
                     /locus_tag="ACHLIF_000806"
     CDS             complement(18334..19194)
                     /gene="mscS"
                     /locus_tag="ACHLIF_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO9604867.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            19390..19488
                     /locus_tag="ACHLIF_000807"
                     /pseudo
     CDS             19390..19488
                     /locus_tag="ACHLIF_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19552..20631)
                     /gene="fbaA"
                     /locus_tag="ACHLIF_000808"
     CDS             complement(19552..20631)
                     /gene="fbaA"
                     /locus_tag="ACHLIF_000808"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO9604868.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(20846..22009)
                     /gene="pgk"
                     /locus_tag="ACHLIF_000809"
     CDS             complement(20846..22009)
                     /gene="pgk"
                     /locus_tag="ACHLIF_000809"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO9604869.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(22059..23078)
                     /gene="epd"
                     /locus_tag="ACHLIF_000810"
     CDS             complement(22059..23078)
                     /gene="epd"
                     /locus_tag="ACHLIF_000810"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO9604870.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            23450..23881
                     /locus_tag="ACHLIF_000811"
     CDS             23450..23881
                     /locus_tag="ACHLIF_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO9604871.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            23904..24482
                     /locus_tag="ACHLIF_000812"
     CDS             23904..24482
                     /locus_tag="ACHLIF_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO9604872.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            24483..25190
                     /locus_tag="ACHLIF_000813"
     CDS             24483..25190
                     /locus_tag="ACHLIF_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO9604873.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATQ
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            25178..25855
                     /locus_tag="ACHLIF_000814"
     CDS             25178..25855
                     /locus_tag="ACHLIF_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9604874.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            25849..26526
                     /locus_tag="ACHLIF_000815"
     CDS             25849..26526
                     /locus_tag="ACHLIF_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9604875.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(26498..27211)
                     /locus_tag="ACHLIF_000816"
     CDS             complement(26498..27211)
                     /locus_tag="ACHLIF_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO9604876.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(27208..27717)
                     /gene="fumE"
                     /locus_tag="ACHLIF_000817"
     CDS             complement(27208..27717)
                     /gene="fumE"
                     /locus_tag="ACHLIF_000817"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO9604877.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(27739..28704)
                     /gene="glpX"
                     /locus_tag="ACHLIF_000818"
     CDS             complement(27739..28704)
                     /gene="glpX"
                     /locus_tag="ACHLIF_000818"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO9604878.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(28701..29978)
                     /gene="yggP"
                     /locus_tag="ACHLIF_000819"
     CDS             complement(28701..29978)
                     /gene="yggP"
                     /locus_tag="ACHLIF_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO9604879.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(29993..31381)
                     /gene="cmtA"
                     /locus_tag="ACHLIF_000820"
     CDS             complement(29993..31381)
                     /gene="cmtA"
                     /locus_tag="ACHLIF_000820"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO9604880.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(31409..31852)
                     /gene="cmtB"
                     /locus_tag="ACHLIF_000821"
     CDS             complement(31409..31852)
                     /gene="cmtB"
                     /locus_tag="ACHLIF_000821"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO9604881.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(32166..34157)
                     /gene="tkt"
                     /locus_tag="ACHLIF_000822"
     CDS             complement(32166..34157)
                     /gene="tkt"
                     /locus_tag="ACHLIF_000822"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO9604882.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            34435..35193
                     /gene="loiP"
                     /locus_tag="ACHLIF_000823"
                     /gene_synonym="yggG"
     CDS             34435..35193
                     /gene="loiP"
                     /locus_tag="ACHLIF_000823"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO9604883.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(35249..35992)
                     /locus_tag="ACHLIF_000824"
     CDS             complement(35249..35992)
                     /locus_tag="ACHLIF_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO9604884.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(35979..37088)
                     /gene="dgaE"
                     /locus_tag="ACHLIF_000825"
     CDS             complement(35979..37088)
                     /gene="dgaE"
                     /locus_tag="ACHLIF_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFO9604885.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(37092..37952)
                     /locus_tag="ACHLIF_000826"
     CDS             complement(37092..37952)
                     /locus_tag="ACHLIF_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO9604886.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(37949..38698)
                     /locus_tag="ACHLIF_000827"
     CDS             complement(37949..38698)
                     /locus_tag="ACHLIF_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFO9604887.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(38724..39209)
                     /locus_tag="ACHLIF_000828"
     CDS             complement(38724..39209)
                     /locus_tag="ACHLIF_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO9604888.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(39220..39648)
                     /locus_tag="ACHLIF_000829"
     CDS             complement(39220..39648)
                     /locus_tag="ACHLIF_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO9604889.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(39767..42565)
                     /gene="dagR"
                     /locus_tag="ACHLIF_000830"
     CDS             complement(39767..42565)
                     /gene="dagR"
                     /locus_tag="ACHLIF_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFO9604890.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(42824..43744)
                     /gene="speB"
                     /locus_tag="ACHLIF_000831"
     CDS             complement(42824..43744)
                     /gene="speB"
                     /locus_tag="ACHLIF_000831"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO9604891.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAFDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(43880..44611)
                     /locus_tag="ACHLIF_000832"
     CDS             complement(43880..44611)
                     /locus_tag="ACHLIF_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9604892.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(44757..46733)
                     /gene="speA"
                     /locus_tag="ACHLIF_000833"
     CDS             complement(44757..46733)
                     /gene="speA"
                     /locus_tag="ACHLIF_000833"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO9604893.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(46742..46873)
                     /gene="yqgB"
                     /locus_tag="ACHLIF_000834"
     CDS             complement(46742..46873)
                     /gene="yqgB"
                     /locus_tag="ACHLIF_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO9604894.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(46967..47110)
                     /gene="yqgG"
                     /locus_tag="ACHLIF_000835"
     CDS             complement(46967..47110)
                     /gene="yqgG"
                     /locus_tag="ACHLIF_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO9604895.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(47221..47472)
                     /gene="yqgD"
                     /locus_tag="ACHLIF_000836"
     CDS             complement(47221..47472)
                     /gene="yqgD"
                     /locus_tag="ACHLIF_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO9604896.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            47528..48682
                     /gene="metK"
                     /locus_tag="ACHLIF_000837"
     CDS             47528..48682
                     /gene="metK"
                     /locus_tag="ACHLIF_000837"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO9604897.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            49118..50512
                     /gene="galP"
                     /locus_tag="ACHLIF_000838"
     CDS             49118..50512
                     /gene="galP"
                     /locus_tag="ACHLIF_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO9604898.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            50589..51086
                     /gene="yggI"
                     /locus_tag="ACHLIF_000839"
     CDS             50589..51086
                     /gene="yggI"
                     /locus_tag="ACHLIF_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO9604899.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            51181..51888
                     /gene="endA"
                     /locus_tag="ACHLIF_000840"
     CDS             51181..51888
                     /gene="endA"
                     /locus_tag="ACHLIF_000840"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO9604900.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            51968..52699
                     /gene="rsmE"
                     /locus_tag="ACHLIF_000841"
     CDS             51968..52699
                     /gene="rsmE"
                     /locus_tag="ACHLIF_000841"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO9604901.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            52712..53662
                     /gene="gshB"
                     /locus_tag="ACHLIF_000842"
     CDS             52712..53662
                     /gene="gshB"
                     /locus_tag="ACHLIF_000842"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO9604902.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            53771..54334
                     /gene="yqgE"
                     /locus_tag="ACHLIF_000843"
     CDS             53771..54334
                     /gene="yqgE"
                     /locus_tag="ACHLIF_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO9604903.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            54334..54750
                     /gene="ruvX"
                     /locus_tag="ACHLIF_000844"
     CDS             54334..54750
                     /gene="ruvX"
                     /locus_tag="ACHLIF_000844"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO9604904.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(54926..55906)
                     /gene="yggR"
                     /locus_tag="ACHLIF_000845"
     CDS             complement(54926..55906)
                     /gene="yggR"
                     /locus_tag="ACHLIF_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO9604905.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLYQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            55924..56628
                     /gene="yggS"
                     /locus_tag="ACHLIF_000846"
     CDS             55924..56628
                     /gene="yggS"
                     /locus_tag="ACHLIF_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO9604906.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            56646..57212
                     /gene="yggT"
                     /locus_tag="ACHLIF_000847"
     CDS             56646..57212
                     /gene="yggT"
                     /locus_tag="ACHLIF_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO9604907.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            57209..57499
                     /gene="yggU"
                     /locus_tag="ACHLIF_000848"
     CDS             57209..57499
                     /gene="yggU"
                     /locus_tag="ACHLIF_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO9604908.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            57507..58100
                     /gene="rdgB"
                     /locus_tag="ACHLIF_000849"
     CDS             57507..58100
                     /gene="rdgB"
                     /locus_tag="ACHLIF_000849"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO9604909.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTHEEKSAISHRGQALKLLLDALRNG"
     gene            58093..59229
                     /gene="hemW"
                     /locus_tag="ACHLIF_000850"
     CDS             58093..59229
                     /gene="hemW"
                     /locus_tag="ACHLIF_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO9604910.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKSSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT        14379 a        16001 c        14866 g        13991 t
ORIGIN      
        1 tcaaaaaaca ctaaatcaaa aattcgtgac attagaaaat ataatgcgaa aacggaggtg
       61 aaattagttt atttcaaatg aggaaaatct cccggcgaaa aaaccgggag atgaaagtgt
      121 gatgggtatc aaataaacag cagaagagaa atttttaacg cagccattca ggcaaatcgt
      181 ttaatcccat tgcctggcgg ataagttgtg gtttaacgcc aggaagcgtg tcggccagtt
      241 tcaaaccaat atcacgcagc agttttttcg ccggattggt accggaaaac agatcgcgga
      301 atccctgcat accagccagc atcagcgccg cactgtgctt gcggctacgc tcataccggc
      361 gcaggtaaat gtactgcccg atgtctttcc cctgacgatg caaccgtttc agttcagcaa
      421 tcagctctgc agcatccata aagccgagat ttactccctg ccccgccagc gggtgaatgg
      481 tatgcgcggc gtcgcccacc agcaccagac ggtgcgcggc aaactggcgc gcataacgcc
      541 ccgtcagtgg gaacacctga cgcgcgctct caaccttgca taagcccagg cgattatcaa
      601 aagcgatatt taacgcgcga ttaaattcat cttcacttgc ctgctgcatc cgctgcgctt
      661 cctctggcga cagtgaccag acaattgagc aaagatgcgg atcgctaagc ggtaaaaagg
      721 ccagaatgcc ttcgccatgg aaaacctgcc gcgccaccgc atcatgcggt tcttccgtgc
      781 gaatggtcgc taccagcgcg tgatgctgat aatcccagaa agtcagcgga atatctgctt
      841 tgttgcgcaa ccaggaatta gcaccgtccg cgccaatcac cagacgcgcc gttaacatgc
      901 tgccatcttt cagcgtcagg aaagtttcat tttctcccca ggcgacctgc tgtaattctg
      961 cgggggccaa cagagtgata tctgacgact gctgcgcttt gttccacagc gcgtagtgaa
     1021 tcactgagtt ttcaacgata tgcccaagat ggctatagcc catgctttga tcgtcaaacg
     1081 aaatgtgacc aaaactgtct ttgtcccaca cttccatacc gtgataacaa ctggccctac
     1141 gagagagaat gtcctgccag acgccaagac gggtgagtaa tttttcgctg gcggcattga
     1201 tagccgaaac gcgcagttgt ggtggtgcat ccgccgccag aggttccggt acgcgctgct
     1261 ccagtacggc aacgcgtaag ccgctcccct gtaagccgca ggcaaccgcc agccccacca
     1321 tgccgccacc aacaatggct acatcaacac tttgcattgt ttattcctta aaaccgcctt
     1381 caacgcgcca cccaaccgag ggtgcgctgc gccagcacat cgcgtgccgg ggtgaataat
     1441 tccatcgtca tcagtccgat gttgcgcccg acaaccagcg gtgcccaacg gttggcaaaa
     1501 agatgtacca ggctgtccgt gacgccgatg gttgcttcgc gatcactctg tcgacgctgc
     1561 tgatagcggc acaatatgcc gtaatcaccg atgtcttctc cgcgctcatg cgcctgagtc
     1621 agggtttccg caaggctcat gacatctcgc ataccgaggt taaacccttg cccggcaatc
     1681 gggtgcagag tttgcgccgc attgcccacc agcacggtac gatgggtaat agctctggcg
     1741 gcgcgggtta acgccagcgg ataagcactg cgtttaccgg cgtgggtaat ttttccaagt
     1801 cgccagccaa aggccgactg gagttcacgg caaaacttct cgtcgctcca cgacagcacc
     1861 tcttcgcgcc gttccagtgg atgacaccag accagcgaac agcgtccgtc agacatcggc
     1921 aacatcgcca gcgggccatg ttgcgtgaag cgttcaaaag cgcggccttc gtgcgcaacg
     1981 gaagtcgcaa cgttggcaat cacggccagt tgttcgtaag gctcctgctg ccagtcaacg
     2041 ccgcacgcgg tggctaacgc tgaatgggtg ccatcagctg ctaccagcac gcggcccgtc
     2101 agcgtctcgc cactctccag cgtcacttca acgtgactct gagtacgggc aacgttagcc
     2161 acgcgatcag ggcaatgcag cgttacgcca ggtgctttac gcagcaatgc aaacagccgt
     2221 tgcccgacat tgtgcaattc gacaacctgt cccagcgccg ccagttggta atcttctgcg
     2281 gcgagggtga caaatccagc gtgaccacga tcgctgacat gcacggtagt gatggcggtt
     2341 gcgtaatccg ccagagattg ccagacgccg atgcgcgcca gttgctgaca ggtccccgca
     2401 gccagcgcaa tagctcgtcc atcaaagccc ggatgagcat gtgactctgg cgcggtcgct
     2461 tcaatcaaat gtaccggcag cgccccgtga cttaaccggg aaatagccag cgccagcgtc
     2521 gcgcccgcca tgccgccacc gacgatgatt acgctcattg ctttctcgca gctgccatca
     2581 acgcttcgat ttcttccggc tttttcacca cgctggcggt gaggttttcg ttgccggttt
     2641 cggtaatcac aatgtcgtct tcaatacgaa tgccgatacc gcgatattgt tctggcacat
     2701 ccgcatccgg cgcgatatac agaccaggct ctacggtcag taccatcccc ggttccagaa
     2761 tgcgcgagcg atcctgacca taaacgccaa cgtcatggac atctagccct aaccagtggc
     2821 taaggccatg cataaagaaa ggacgatggg cgttctgagc gatcagttca tcaacatcac
     2881 ctttcagaat gccgagtttt accaggccgc taaccatgat gcgcaccact tcaccagtga
     2941 cttccagaat ggaagttccc ggacgataca ggcgcaagct ggtttcgaga gactccagca
     3001 caatgtcgta gatttcacgc tgggcctgag tgaatttgcc gttgaccggg aaggtgcggg
     3061 taatatcgcc agcgtaacct ttgtattcgc aacccgcgtc aatcaacacc aggtcgccgt
     3121 cgcgcatttc acactcgttt tcggtgtagt gcagaatgca gccgttttca ccgctgccga
     3181 caatggtgtt ataggacgga tagcgcgcac cgtggcggtt aaattcgtgg tgaatttcgc
     3241 cttccagatg gtactcgaac attcccggac ggcatttttc catcgcccgg gtatgtgcca
     3301 tggcggtgat ttctcccgcg cggcggagta cggcaatctc ttctggcgat ttgaacaggc
     3361 gcatttcatg aacaacagga cgccagtcga tcatcgttgc cggtgcggtg agattttgcc
     3421 gcgaaccttt acgcagtttt tccagcgcac tgttcacgat ttcatcagca tatgcatatt
     3481 cgccctgggc atggtaaacc acatcctggc cgttaagtag ttgataaagt tgctgattga
     3541 tttcgctgaa tgccagtgcg cggtcaacgc ccagtttctc tggcgcggca tcctggccta
     3601 agcgacggcc aaaccagatc tccgccgtca ggtcgcgaac gcggttaaac agaacgctgt
     3661 ggttatgagt gtcatcgctt ttaatcagca ccagcaccgc ttccggttcg ttaaagccgg
     3721 tgaagtacca gaagtcactg ttctgacgat aggggtattc gctgtcggcg ctacgtgtta
     3781 cttctggtgc agcaaaaatc agcgcggcgc tgccgggttg catttgctcc accagggcct
     3841 gacggcgacg ctgaaactct tgccgggata tctcactcat aacactctcc ttacgttttt
     3901 tgtttttagt gtagagtcgg tttttgtact tctggcgcgg tcggttgcgg atgagtaaag
     3961 gtgtcgtggc ataacagcgc ggcaacacga acgtattcga tgatctcttc aagcgacatt
     4021 tcaagctctt cctgatcttc gtcttcgtcg taacccagtt gcgcaatgtt acgcagatcg
     4081 tcgatagctt caccggtttc gccggtcact ttatccagct tcggttgcgt aacgccaaga
     4141 ccaagcagga agtgattgac ccaacctgcc aatgcatcag cccgatcgaa aacgctgaca
     4201 tcatcgccat caggcagata aagctgaaaa aggaagccgt catcctgcag ggcatcgctg
     4261 gtggcagagt gcattttacg cagtgcctgt gccagctcat gaccgaaagc catgccttcg
     4321 ttcgtcaggt cgtgaagtag cggtagccat gagctgtcat cgttaccgcc acatatcatc
     4381 ccactgatta aaccatgcat ctcagcaggg gtcagacccg tcccttgttg gttcagatac
     4441 tggttcattt cgttgtaacc aggcatttcg ttctgtatag acataagcat tcgtcgtcaa
     4501 agggaggaat attcatgata tgctaccact ttgggccctg gtggaccaga aaagggcttg
     4561 tctcttctca tcagggtagc tatagtgtcg ccccttcgca gaccatgggt ctaaagacga
     4621 aggcagcgca gtcaatcagc aggaaggtgg catgtctgca caacccgtcg atatccaaat
     4681 ttttggccgt tcactgcgtg tgaactgccc gcctgaccaa agggatgcgt tgaatcaggc
     4741 agcggacgat ctgaaccaac ggttgcaaga tctgaaagaa cgcactagag tcacaaatac
     4801 tgaacagttg gtcttcattg ccgcattgaa tatcagctat gagttagcgc aagaaaaagc
     4861 gaagactcgt gactacgcgg caagtatgga acagcgtatt cggatgctgc agcagaccat
     4921 agaacaagcg ttacttgaac aaggtcgcat caccgaaaaa actaaccaaa actttgaatg
     4981 acacttttcg gtttactgtg gtagagtaac cgtgaagaca aaatttctct gagatgttcg
     5041 caagcgggcc agtcccctga gccgatattt cataccacaa gaatgtggcg ctccgcggtt
     5101 ggtgagcatg ctcggtccgt ccgagaagcc ttaaaactgc gacgacacat tcaccttgaa
     5161 ccaagggttc aagggttaca gcctgcggcg gcatctcgga gattcccttc ttatctggca
     5221 ccagccatga cgcaactacc agaactccca ctgacattat cccgacaaga aatccgcaaa
     5281 atgattcggc aacgtcgtcg tgcgttaacg ccggaacaac agcaggaaat gggtcaacaa
     5341 gccgctaccc ggatgatgac ttatcccccg gtggtgatgg cgcatacggt cgctgtattc
     5401 ctctcttttg atggtgaact cgacacccag ccgctaatag aacaactctg gcgcgccggt
     5461 aagcgcgtat atcttccagt tttgcatccc tttagtgccg gtaatttgct gttcctgaat
     5521 taccatccgc aaagcgaact ggtgatgaac aggttgaaga tccatgagcc aaaactggat
     5581 gtgcgtgacg tgctacccct ttcccgatta gacgtgctga tcacaccgct ggtcgccttt
     5641 gatgagtacg gtcagcgcct gggaatgggc ggtggttttt atgatcggac cttacaaaac
     5701 tggcagcact ataaaacgca accggtgggt tatgcgcatg attgtcagtt ggtggaaaaa
     5761 ctccccgttg aagagtggga tatccctctt cctgcggtgg ttacaccgtc gaaagtctgg
     5821 gagtggtaag ggcgatacac ctgcatcgcc ctgattgaca tcgttgattc tttaacctaa
     5881 tttagtgagt aagggtaagg gaggattgct cctcccctgt gactgactgt taataagctg
     5941 ggaaacttat gagtaacagt acaatcagta tgatggcaag tcgcatcata acccttctcc
     6001 ttcaagccct cgcttcggtg agggctttac cgttacagcc ccatgctgcc cagctatcgt
     6061 aagctctctt taaataacca caacgcattg atctaccttt aaatcttttc ttcgagcgtg
     6121 ctcgaaaaac cccatgcaaa acagagataa tttttgggaa aaaggatcta ttacggcagt
     6181 aaacgtgggc gatgaagtgg ctatcaaaaa gttcggttca gggcgatgca ccccgcatcg
     6241 ccctgattga catcgttgat tctttgacct aatttagtga gtaagggtaa gggaggatga
     6301 ctcctcccct gagactgact gttaataagc tccgaaactt atcagtaaca gtacaatcag
     6361 tatgatgaca agtcgcatca taacccttct cctttaagcc ctcgcttcgg tgagggcttt
     6421 accgttacag ccccatgctg tcccgccatc gtaaatcccc attaaataaa cacaacgcat
     6481 tgatctgact ttgatttatt ttctggagca gactcgcaaa gtagaatgcg caacgcggca
     6541 acggtgtgga gaagggataa aaaaacgggc aagtcagtga cctgcccgtt aattttcaga
     6601 gaaggggaat tagtacagca gacgggcgcg aatggtaccc ggaatagctt tcattgcctg
     6661 cagcgctttt tcggcaacgt cttcgtcagc ttcaatatca ataactacat aacccatctg
     6721 ggcggaagtt tgtagatatt gcgcggcgat gttgacgccc tgctcggcga agattttgtt
     6781 cagcgcagtt agcacgcccg gacggttttc gtggatgtgc atcagacgac gaccaccatg
     6841 cagtggcagc gagacttccg ggaagttcac cgcagagagc gttgagccat tgtcagaata
     6901 cttgatcaat ttacccgcaa cttccaggcc gatattctcc tgcgcttcct gagtcgaacc
     6961 gccaatgtgt ggcgtcagaa ggacgttgtc gaattcacac agtggagagg taaatggatc
     7021 gctattggtc gccggttccg tcgggaatac gtcgattgcc gcccccgcca gatgtttgct
     7081 cgccagcgca tcacacagcg ccggaatatc caccacagta ccgcgcgagg cattaatcag
     7141 cagcgagccg ggcttcatta gcgaaatctc tttcgcgccc atcatatttt tggtggacgg
     7201 attctctggt acatgcagac tcaccacatc gctcatattc agcaggtcag aaagatgctg
     7261 tacctgagtg gcgttgccca gcggcagttt gttttcaata tcataaaagt aaacatacat
     7321 tcccagcgat tcagccagaa tgcccaattg cgtaccaata tgaccgtagc cgatgatacc
     7381 cagctttttg ccgcgcgctt caaaagaacc cgccgccagt ttgttccaca cgccacggtg
     7441 cgctttagca ttggcttccg gcacgccgcg caatagcagc agcagttcgc caatcaccag
     7501 ctccgcaaca gagcgcgtat ttgagaacgg tgcgttaaat accgggatcc cgcgctttgc
     7561 cgccgcatcc agatcaacct ggtttgttcc gatacagaaa cagccaatag cgaccagttt
     7621 ttctgcggcg ttgatcacgt cttcagtcag atgggtacgg gatcgcaggc cgatgaagtg
     7681 ggcatcgcgg atggattctt ttaattgttc atcatccagc gcgcctttgt gaaattcgat
     7741 gttggtgtaa ccagctgcac gaaggctttc cagcgccttt tggtgcacgc cttctaccag
     7801 cagaaactta atcttgtctt tctccagcga tacctttgcc atttacccaa tcctgtcttt
     7861 tgaaatgttg tgtgcggatt tgcatccgcc tttcaacata tcaaaaaata atattgcggc
     7921 aatatgaacg tttgcgtcgc gatgttgagg aaatatcacg caaaggtaat tcttggatga
     7981 atatgctggt ttagaggatt tatttagagc aatcgacaat tgcctggtaa aagcgtgaca
     8041 catgtcacca aatttaatga agagaatttt tttaacgggg gaggttcccc cgtcagatca
     8101 tttcacaatg gttttgacac cgtcaggtgt gccaatcagc gcaacgtccg cgccacggtt
     8161 agcaaacaag ccaacagtca ccacgccagg aatcgcattt atggcgtttt ccatcgctat
     8221 cgggtcaagg atttccatgc cgtggacgtc gaggatcacg ttgccattat cggtcaccac
     8281 gccctgacgg tattccggac gaccgcccag tttcaccagc tgacgcgcca ctgcactacg
     8341 tgccatcggg ataacttcta ctggcagcgg gaatttaccc agaatatcaa cctgcttgga
     8401 agcgtctgca atacagataa atttttctgc aaccgaagca atgatttttt cacgggtcag
     8461 cgccgcgccg ccgcctttga tcatttgcat gtggccgttg atttcatctg cgccatcaac
     8521 gtagatgcca aggctgtcga cttcgttgag atcaaaaacg tgaatgccga ggcttttcag
     8581 tttttcagtg gaagcatctg aactggaaac ggccccttca atctggcctt tcattgtacc
     8641 gagcgcgtca ataaagtgtg cggcggtgga acctgtacct acaccaacaa tggtgccggg
     8701 ctgaacatac tgaagtgccg cccatcctac tgcttttttc aattcatcct gcgtcatgat
     8761 cgtttcgcct gtggtatgaa atttcacacg cattatatac aaaaaaagcg attcagaccc
     8821 cgttggcaag ccgcgtggtt aactcatcca taaaatatcg cgcaatggcg ggcatcccct
     8881 ttcgccccgc aaataaagca tacaacggtc tgggtatgcc gctccacggt gcgaacagag
     8941 gcaataacgc cacgccattc aacacaacca gggcgcgaac tcgctgagcg aaatgcatta
     9001 gcgcaaatcc ctgatctttt aagctgcgcg tttaaccacg aatacaaatg ttacccgccg
     9061 acggcttcgg tatatgcaac ctgacacaaa attgtgtcat agtgcaggaa aaagcattta
     9121 ccaggagcag acaacagcaa tgaaacgccc ggactacaga acattacagg cactggatgc
     9181 ggtgatacgt gaacgaggat ttgagcgcgc ggcacaaaag ctgtgcatta cacaatcagc
     9241 cgtctcacag cgcattaagc aactggaaaa tatgttcggg cagccgctgt tggtgcgtac
     9301 cgtaccgccg cgcccgacgg aacaagggca aaaactgctg gcactgctgc gccaggtgga
     9361 gttgctggaa gaagagtggc tgggcgatga acaaaccggt tcgactccgc tgctgctttc
     9421 actggcggtc aacgccgaca gtctggcgac gtggttgctt cctgcactgg ctcctgtgtt
     9481 ggctgattcg cctatccgcc tcaacttgca ggtagaagat gaaacccgca ctcaggaacg
     9541 tctgcgccgc ggcgaagtgg tcggcgcggt gagtattcaa catcaggcgc tgccgagttg
     9601 tcttgtcgat aaacttggtg cgctcgacta tctgttcgtc agctcaaaac cctttgccga
     9661 aaaatatttc cctaacggcg taacgcgttc ggcattactg aaagcgccag tggtcgcgtt
     9721 tgaccatctt gacgatatgc accaggcctt tttgcagcaa aacttcgatc tgcctccagg
     9781 cagcgtgccc tgccatatcg ttaattcttc agaagcgttc gtacaacttg ctcgccaggg
     9841 caccacctgc tgtatgatcc cgcacctgca aatcgagaaa gagctggcca gcggtgaact
     9901 gattgactta acgcctgggc tatttcaacg acggatgctc tactggcacc gctttgctcc
     9961 tgaaagccgc atgatgcgta aagtcactga tgcgttactc gattatggtc acaaagtcct
    10021 tcgtcaggat taatccatca aataatgcct gatagcacat atcaggcgtt gtcctcactt
    10081 ctttttgtat tccttgaatc acatcacaaa atagacaaat ctcaggcggc aaaaaacgac
    10141 gtctgaatgc attttttttg ctggcgacaa acccacgtaa aaagctcacc gtaggcgcaa
    10201 ataccctcat tttgattgcg ttttacggag caaataatgt ctaacgtgca ggagtggcaa
    10261 cagcttgcca acaaggaatt gagccgtcgg gagaaaactg tcgactcgct ggttcagcaa
    10321 accgcggaag ggatcgccat caagccgctg tataccgaag ccgatctcga taatctggag
    10381 gtgacaggta cccttcctgg tttgccgccc tacgttcgtg gcccgcgtgc cactatgtat
    10441 accgcccaac cgtggaccat ccgtcagtat gctggttttt caacagcaaa agagtccaac
    10501 gctttttatc gccgtaacct ggccgccggg caaaaaggtc tttccgttgc gtttgacctt
    10561 gccacccacc gtggctacga ctccgataac ccgcgcgtgg cgggcgacgt cggcaaagcg
    10621 ggcgtcgcta tcgacaccgt ggaagatatg aaagtcctgt tcgaccagat cccgctggat
    10681 aaaatgtcgg tttcgatgac catgaatggc gcagtgctac cagtactggc gttttatatc
    10741 gtcgccgcag aagagcaagg tgttacacct gataaactga ccggcaccat tcagaacgat
    10801 atcctcaaag agtacctctg ccgcaacacc tatatttacc caccaaaacc gtcaatgcgc
    10861 attatcgccg acatcatcgc ctggtgttcc ggcaacatgc cgcgatttaa taccatcagt
    10921 atcagcggtt accacatggg ggaagcgggt gccaactgcg tgcagcaggt agcatttacg
    10981 ctcgctgatg ggattgagta catcaaagca gcaatctctg ccggactgaa aattgatgac
    11041 ttcgctcctc gcctgtcgtt cttcttcggt atcggcatgg atctgtttat gaacgtcgcc
    11101 atgttgcgtg cggcacgtta tttatggagc gaagcggtca gtggatttgg cgcacaggat
    11161 ccgaaatcac tggcgctgcg tacccactgc cagacctcag gctggagcct gactgaacag
    11221 gatccgtata acaacgttat ccgcaccacc attgaagcac tggctgcgac gctgggcggt
    11281 actcagtcac tgcataccaa cgcctttgac gaagcgcttg gtttgcctac cgatttctca
    11341 gcacgcattg cccgcaacac ccagatcatc atccaggaag aatcagaact ctgccgcacc
    11401 gtcgatccac tggccggatc ctattacatt gagtcgctga ccgatcaaat cgtcaaacaa
    11461 gccagagcta ttatccaaca gatcgacgaa gccggtggca tggcgaaagc gatcgaagca
    11521 ggtctgccaa aacgaatgat cgaagaggcc tcagcgcgcg aacagtcgct gatcgaccag
    11581 ggcaagcgtg tcatcgttgg tgtcaacaag tacaaactgg atcacgaaga cgaaaccgat
    11641 gtacttgaga tcgacaacgt gatggtgcgt aacgagcaaa ttgcttcgct ggaacgcatt
    11701 cgcgccaccc gtgatgatgc cgccgtaacc gccgcgttga acgccctgac tcacgccgca
    11761 cagcataacg aaaacctgct ggctgccgct gttaatgccg ctcgcgttcg cgccaccctg
    11821 ggtgaaattt ccgatgcgct ggaagtcgct ttcgaccgtt atctggtgcc aagccagtgt
    11881 gttaccggcg tgattgcgca aagctatcat cagtctgaga aatcggcctc cgagttcgat
    11941 gccattgttg cgcaaacgga gcagttcctt gccgacaatg gtcgtcgccc gcgcattctg
    12001 atcgctaaga tgggccagga tggacacgat cgcggcgcga aagtgatcgc cagcgcctat
    12061 tccgatctcg gtttcgacgt agatttaagc ccgatgttct ctacacctga agagatcgcc
    12121 cgcctggccg tagaaaacga cgttcacgta gtgggcgcat cctcactggc tgccggtcat
    12181 aaaacgctga tcccggaact ggtcgaagcg ctgaaaaaat ggggacgcga agatatctgc
    12241 gtggtcgcgg gtggcgtcat tccgccgcag gattacgcct tcctgcaaga gcgcggcgtg
    12301 gcggcgattt atggtccagg tacacctatg ctcgacagtg tgcgcgacgt actgaatctg
    12361 ataagccagc atcatgatta atgaagccac gctggcagaa agtattcgcc gcttacgtca
    12421 gggtgagcgt gccacactcg cccaggccat gacgctggtg gaaagccgtc acccgcgtca
    12481 tcaggcacta agtacgcagc tgcttgatgc cattatgccg tactgcggta acaccctgcg
    12541 actgggcgtt accggcaccc ccggcgcggg gaaaagtacc tttcttgagg cctttggcat
    12601 gttgttgatt cgagagggat taaaggtcgc ggttattgcg gtcgatccca gcagcccggt
    12661 cactggcggt agcattctcg gggataaaac ccgcatgaat gacctggcgc gtgccgaagc
    12721 ggcgtttatt cgcccggtac catcctccgg tcatctgggc ggtgccagtc agcgagcgcg
    12781 ggaattaatg ctgttatgcg aagcagcggg ttatgacgta gtgattgtcg aaacggttgg
    12841 cgtcgggcag tcggaaacag aagtcgcccg catggtggac tgttttatct cgttgcaaat
    12901 tgccggtggc ggcgatgatc tgcagggcat taaaaaaggg ctgatggaag tggctgatct
    12961 gatcgttatc aacaaagacg atggcgataa ccataccaat gtcgccattg cccggcatat
    13021 gtacgagagt gccctgcata ttctgcgacg taaatacgac gaatggcagc cacgggttct
    13081 gacttgtagc gcactggaaa aacgtggaat cgatgagatc tggcacgcca tcatcgactt
    13141 caaaaccgcg ctaactgcca gtggtcgttt acaacaagtg cggcaacaac aatcggtgga
    13201 atggctgcgt aagcagaccg aagaagaagt actgaatcac ctgttcgcga atgaagattt
    13261 cgatcgctat taccgccaga cgcttttagc ggtcaaaaac aatacgctct caccgcgcac
    13321 cggcctgcgg cagctcagtg aatttatcca gacgcaatat tttgattaaa ggaattttta
    13381 tgtcttatca gtatgttaac gttgtcacta tcaacaaagt ggcggtcatt gagtttaact
    13441 atggccgcaa acttaatgcc ttaagtaaag tctttattga tgatcttatg caggcattaa
    13501 gcgatctcaa ccggccggaa attcgctgta tcattttgcg cgcgccgagt ggatccaaag
    13561 tcttctccgc aggccacgat attcacgaac tgccgtctgg cggtcgcgat ccgctctcct
    13621 atgatgatcc attgcgtcaa atcacccgca tgatccaaaa attcccgaaa ccgatcattt
    13681 cgatggtgga aggtagtgtt tggggtggcg catttgaaat gatcatgagt tccgatctga
    13741 ttatcgccgc cagtacctca accttctcaa tgacgcctgt aaacctcggc gtcccgtata
    13801 acctggtcgg cattcacaac ctgacccgcg acgcgggctt ccacattgtc aaagaactga
    13861 tttttaccgc ttcgccaatc accgcccagc gcgcgttggc tgtcggcatc ctcaaccatg
    13921 ttgtggaagt ggaagaactg gaagatttca ccttacaaat ggcgcaccac atctctgaga
    13981 aagcgcagtt agccattgct gttatcaaag aagagctgcg tgtactgggc gaagcgcaca
    14041 ccatgaactc cgatgaattt gaacgtattc aggggatgcg acgcgcggtt tatgacagcg
    14101 aagattacca ggaaggaatg aacgctttcc tcgaaaaacg taaacctaat ttcgttggtc
    14161 attaatccct gcgaacgaag gagtaaaaat ggaaactcag tggacaagga tgaccgccga
    14221 tgaagcggca gaaattatcc agcataacga catggtggca tttagcggct ttaccccggc
    14281 gggttcgccg aaagccctac ccaccgcgat tgcccgcaga gctaacgaac agcatgaggc
    14341 caaaaagccg tatcaaattc gcctgctgac gggggcgtca atcagcgccg ccgctgacga
    14401 tgtactttct gacgccgatg ctgtttcctg gcgtgcgcca tatcaaacat cgtccggttt
    14461 acgtaaaaag atcaatcagg gcgcggtgag tttcgttgac ctgcatttga gcgaagtggc
    14521 gcaaatggtc aattacggtt tcttcggcga cattgatgtt gccgtcattg aagcatcggc
    14581 actggcaccg gatggtcgag tctggttaac cagcgggatc ggtaatgcgc cgacctggct
    14641 gctgcgagcg aagaaagtga tcattgaact taatcactat cacgatccgc gcgttgcaga
    14701 actggcagat attgtgattc ctggcgcgcc accgcggcgc aatagcgtgt cgatcttcca
    14761 tgcaatggat cgcgtcggta cccgctatgt gcaaatcgat ccgaaaaaga ttgtcgccgt
    14821 cgtggaaacc aacttgcccg acgccggtaa tatgctggat aagcaaaatc ccatgtgcca
    14881 gcagattgcc gataacgtgg tcacgttctt attgcaggaa atggcgcatg ggcgtattcc
    14941 gccggaattt ctgccgctgc aaagtggcgt gggcaatatc aataatgcgg taatggcgcg
    15001 tctgggggaa aacccggaaa ttcctccgtt catgatgtat tcggaagtgc tacaggaatc
    15061 ggtggtgcat ttactggaaa ccggcaaaat cagcggggcc agcgcctcca gcctgacaat
    15121 ctcggccgat tccctgcgca agatttacga caatatggat tactttgcca gccgcattgt
    15181 gttgcgtccg caggagattt ccaataaccc ggaaatcatc cgtcgtctgg gcgtcatcgc
    15241 tctgaacgtc ggcctggagt ttgatattta cgggcatgcc aactcaacgc acgtagccgg
    15301 ggtcgatctg atgaacggca tcggcggcag cggtgatttt gaacgcaacg cgtatctgtc
    15361 gatctttatg gctccgtcga ttgctaaaga aggcaagatc tcaaccgtcg tgccaatgtg
    15421 cagccatgtt gatcacagcg aacacagcgt caaagtgatc atcaccgaac aagggatcgc
    15481 cgatctgcgc ggtctttccc cgcttcaacg cgcccgcact atcattgata attgtgcaca
    15541 tcctatgtat cgggattatc tgcatcgcta tctggaaaat gcgcccggcg gacatattca
    15601 ccacgatctt agccacgtct tcgacttaca ccgtaattta attgcaaccg gctcgatgct
    15661 gggttaatgg tttgtcccgt cacgatcttc agcaatatgc tgaagcatcg tggcggtata
    15721 acgatgattt ctgattgaat aaagatattc ctgcatatac atcggcctgt gcggcgcatc
    15781 aaaatatttc agcgttgccg gattcaccag tcgccaggca aaatgcggga tcaccgtcag
    15841 aaaatgtccg cgctccacgg cgctgatttt ggccataaaa ctgtagggac gataaataat
    15901 ggtgggattg atcccacaag gtcgcatatt cgcatcaatc attgcttcga agttagcccg
    15961 gttttgaaag cgcatttgta gccagggcag ctcctgaagc agctcttgct cttgacgctc
    16021 ttcataacgg cgagaaacga gaaagcccaa tcgcagcggt ggtaattcac tgatagtcag
    16081 gttttccagt tcctgaactc gtgcagatac atgctgtggg gagataataa aatctaactg
    16141 acggtcaaaa agattatcga taacaccgtt ttcgctgaac tcaactggct gggcggtcac
    16201 gccttcatac ttatctccga gactaatcaa ctgatcgaaa ataattgtcg gataagtatt
    16261 atctatgcca atcacaattt ctcgtgaacg acgccctgag ttatggattt cattatcaat
    16321 cgcagaaagt cgttgataaa ccgggaacag tttttgatac agttcctgac cggctttatt
    16381 gaggctgata ttattatctt tgcgggtaaa tagcgtatag ccaatttgtt cttccagcgc
    16441 ggcaatgctt ttaccaaatg gcgaagccgt catatggatt ttttctgccg cccgggcaat
    16501 attattggtt tgcgctaata aaatgaaatt gcgcatcttt ttcgaaataa aaatgtccat
    16561 cccccctccc cgcatccttg tccgcttaac cgtttcagtc agcctatcct tgatgaaacc
    16621 gcgagcaaag ataggtgatt acgtcatggt tttacagaaa attacagaaa aaggaggcaa
    16681 tatcgggtaa aggcattagc ccgacgaata cgtcgggcta caagtattat tgtgctgcag
    16741 gtgttttagc gggttgttga tccacaggtt ctaactggaa gaccacatcg acctgatcat
    16801 caaactgaat agcggcctgc tcgtaagttt cctgggcaga caccggcgcg gcatcggctt
    16861 tcatcatccg caccattggg ctgggctgat agttggaaac atggtagcgc acgctatata
    16921 ccggccccag tttacgatga aagccgttcg ccagttcctg cgcctgatga atcgcgttat
    16981 caatcgctgc cttacgcgct ttgtctttat aggcatccgg ctgcgccacg cccagcgaca
    17041 cagaacgaat ttcgttaaga cccgccttca gcgcgccatc cagcaaggaa ttcagtttgt
    17101 ctaactgacg gagcgtgact tccaccgttc tcacagcgcg gtagcctttc aggatacttt
    17161 taccatcctg ataatcataa tctggctggg tgcgtaaatt cgctgagctg atatcttttt
    17221 tcgcgatctg attgagttca aggaaggaaa tgtattgtgc gacgcgctca tctgcctgtt
    17281 tcttggctgt agcggcatcc ttcgcggcca cgttaacttc aatcgcaaga gtggcaatgt
    17341 ctggcaccgc atccacactt gccgtaccgg aggtgacaat atgcggtcca tccggcaatt
    17401 cgttagcctg cgctgccatc ccgctaatac ccattaatgc cgccagggcg ataactttga
    17461 acttcacgtt agtctctccg tgttaattga cttgcccaaa attcaggacc tctggaggca
    17521 agcttagcgc ctctgtttta tttttccatc agatagcgcc taactgaaca aggcttgtgc
    17581 atgagcaata ccgtctctcg ccagctgcaa ggctataaac cacatgacac atcccactac
    17641 cagattgata atgcgctgtg attttgccgt gcgcaggcgc ggtgccagcc aggctgcgag
    17701 aatagccaga ccaaagaacc acaggaaaga ggcgctaatt gtcccgagcg caaaccaacg
    17761 ttttggttcg acgtcaagct gcccgccaag gctgcccagt acaacaaaag tatccaggta
    17821 aacatgcgga ttcagccagg tcactgccaa catggtggcg ataattttcc atctgccttg
    17881 cttcaggact tcggcactag ctaactcaat attactactc atcgctgttt taaaagcgcc
    17941 aaaaccatac cacagcaaga agactacgcc gccccaggtg accagcgcca gcaaccacgg
    18001 cgactgcatc aataacgcgc taccaccaaa aatccccgcg caaatcagga ccaaatcgct
    18061 gatagcgcaa agtaaggcaa tcataatgtg gtactgacga cgaatgccct gattcatcac
    18121 aaaagcattt tgtgggccga gcggcagaat catagctgcc ccaagagcaa gaccttgaaa
    18181 gtaataagaa aacacgttgg atattccgaa ttaagtatct gatgcagaga ctatatcgcg
    18241 ggattgttat tagcggaaat tgataatatt aattggcaat cagaataact aataagagag
    18301 aggccagaga gtattatctg gcctcagcgt tgattacgca gctttgtctt ctttcacccg
    18361 cttaaagttc acatccattt gcgggtacgg gaagctgata ccggcggcat caaattcacg
    18421 tttaatacgc tccagcacat cccagtacac gttttgcaga tcgccgctgt tgctccagac
    18481 gcggaccacg aaattaatcg acgatgcacc aagttcgttc aggcgcacag tcatttcgcg
    18541 atctttcaaa atgcgatctt cagactggat aatattggtc aggatctgct taacctgatc
    18601 gatatcggaa tcatacgcca cgccaataat aaattcgtta cggcgaactg gctcgcggga
    18661 gaagttaata atatttccgg caataatttt accgttcgga ataacgataa ttttaccgtc
    18721 tgcagtacgc atggtggtgg agaaaatctg cacactcagc acagtaccgg ctacgccgcc
    18781 gaggtcaaca tattctccgg cacggaacgg gcggaacatg acaagtaaca cgccagcggc
    18841 caggttagaa agtgacccct gcaaagccag accaacagct aagcctgcgg caccgagtac
    18901 agcaatgact gacgctgttt gtacacccac gcgtcccagg gctgcgatta gcgtaaaggc
    18961 gataataccg taacggacta atgcagaaag aaaatcagca acagtggcat cgattttacg
    19021 ggagatcatc aggcgattca ccgcgttgga aatcatccgc gcgataatca aaccaacgat
    19081 gatgatcgcg agtgccgcca cgatgtttac tgcataactt agcagcagcg cctggttagc
    19141 taccagccag cttcccgcgc cgtttatgct atcgacaaca ttcaaatctt ccattcaata
    19201 ttccttttca actgactcaa aatggagaaa tacactccgc cgccttatga cgggcagtct
    19261 gacaagggta aacaataaag cataattttg ccaaatagat cacagatttc tcatctgaat
    19321 caccttgctt ttggcgaaaa aatccccgca gagcggggct cagacagata tcaatgtgcg
    19381 ctttgtcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaaacc tgcaaattca
    19441 atatattgca ggatacgtgt aggcctgata agcgtagcgc atcaggcaat tttgcttttg
    19501 gcgaaaaaaa gacccgcaga gcgggccttg agataagcag aaaggaatga cttacagaac
    19561 gtcgatcgcg ttcagttcct ggaatgcttt ttccagacga gcgatcatcg aagtctgacc
    19621 ggcacgcagc catacgcgcg gatcgtagta tttcttgttc ggctgatctt cgcctttcgg
    19681 gttacccagc tgaccctgca gataagcttc gttcgctttg tagtagttca gaacgccttc
    19741 ccaggttgcc cattgggtat cggtatcgat gttcattttc actacgccgt agcttacgga
    19801 gtctttgatt tcctgagcag tagaaccgga accaccgtgg aatacgaagt tcaggctgtt
    19861 gtgcggcagg ttgtgtttct tagaaacata ttcctgagaa tcacgcagga tggtcggagt
    19921 cagaaccacg ttacccggct tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt
    19981 gaaacgcggg ctgattttgc tcagctcggt gtatgcgtaa tcaacgtctt ccggctgggt
    20041 gtacagtgca gaagcgtcca tgtggctgtt gtccacgccg tcttcttcac caccggtgca
    20101 acccagttcg atttccagag tcatgccgat tttggacata cgctccaggt atttagagca
    20161 gatttcgatg ttctcttgca gagattcttc agacaggtcg atcatgtgag aagagaacag
    20221 cggcttaccg gtagctgcga agtgtttttc acccgcgtcc agcagaccgt cgatccacgg
    20281 cagcagtttc ttcgcgcagt ggtcagtgtg caggataacc ggaacaccat aatgttcagc
    20341 catctggtga acgtgatgcg caccagagat cgcgcccagg atagcagcac cctgcggaac
    20401 gtcagatttc acgcctttac cagcgataaa ggaagcacca ccgttggaga actgaacgat
    20461 aaccggcgct ttaactttag cagcagtttc cagtacggcg ttgatggagt cagtaccgac
    20521 gcagtttact gctggcagtg cgaagttgtt ttcttttgct acctggaaaa ctttctgtac
    20581 gtcatcacca gtgattacgc caggttttac gaaatcaaaa atcttagaca tgtctcttgt
    20641 cctgtatcgt cgggccttat actcatcata cttcaagttg catgtgctgc gtctgcgttc
    20701 gctcacccca gtcacttact tatgtaagct cctggggatt cactctcttg tcgccttcct
    20761 gcaactcgaa ttatttagag tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg
    20821 gcaggtttcc ctgccctgtg attttttact tcttagcgcg ctcttcgagc atcgctactg
    20881 caggcagtac tttaccttcc acgaattcga ggaatgcgcc gccgccagtg gagatgtagg
    20941 agattttgtc agcaatgccg aacaggtcga ttgctgccag agtgtcgccg ccgccagcga
    21001 tggagaacgc ttcgctgtct gcgatagcgt tagccacgat ttcagtacct ttgcggaagt
    21061 tcgggaattc gaacacgcca accggaccgt tccacagaat ggttttcgca ttcttcagga
    21121 tttcagccag ttcctgagcg gaagcatcac cgatatccag gatctgctcg tcagctttca
    21181 catcgttaac agatttcagg gtagccggtg cagtttcaga gaactcggtt gctacgcgaa
    21241 catcagacgg aaccgggatg ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt
    21301 cagcttcgta cagggattta cccacatcgt ggccttgtgc cgcgataaag gtgttagcga
    21361 taccaccacc aacaatcagc tggtcagcga ttttagacaa ggagtccaga acggtcagtt
    21421 tggtagatac tttagaacca ccaacgatag ccaccatcgg gcgagctggt tctttcagtg
    21481 ctttacccag cgcgtccagt tcagctgcca gcagcgggcc tgcgcacgca acgtcagcga
    21541 atttaccgat accgtgagta gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta
    21601 cgaacacgtc acacagtgca gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc
    21661 ctttgttgaa gcgaacgttt tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt
    21721 cgaggtaatc tttaaccagg cgaaccgggt tagacagttt gtctttcagg tagttaacaa
    21781 ccggcagcag agagaattct tcgttgtact cgccttcggt aggacgaccc aggtgggaag
    21841 ttaccatcac ttttgcgcct tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga
    21901 tacgcgcgtc gctggttact ttcccgtctt ttactggtac gttcagatcc gcacggataa
    21961 atacacgttt cccagcaaga tccagatcgg tcatcttaat tacagacatg gtgaatcctc
    22021 tcgttgattc taaaagtttt gcagacgctg cttgcgtctt acctgaaagc aacagtagcc
    22081 atagctaacg tcgtgtcgag cattcggtta gcaaagcccc attcgttatc gcaccagacc
    22141 aacgttttga tcaggtgtgc gccactgacc cgggtttggg tgccatcgac aatggcactg
    22201 tgcggatcgt ggttaaaatc tacagagacc aacggcaatt ccgtatagtc aactatacca
    22261 tgaaatgcac cttgtgctgc tttttgcagc aacaggttga cttcattggc ttttacaggt
    22321 ttcttcaccg tcacgcttaa atcgattgcc gtcacattta tggttggcac acgtaccgca
    22381 atcgcttcaa agcgatcgtt aaattgcgga aaaaatcgtg tgataccggc ggccagttta
    22441 gtatcgaccg gaatgatcga ctggctggct gcccgggtgc gacgcaggtc aggatggtat
    22501 gcatcaataa cctgttgatc gtgcatggcg gagtgaattg tggtcacagt gccggactca
    22561 ataccgtacg catcatctaa caatttgatg acgggaatta tgcaattcgt ggtacaggaa
    22621 gcgttagaaa cgatgcggtg ttccgcacga agttgatcct gattgacgcc gtaaacaacg
    22681 gtcgcgtcga gatcgttact gccaggatgt gaaaagagca cttttttggc cccggcggca
    22741 atatgcgctt cgccatgctc gcgggagcca tatacgccgg tgcagtcgag gactacatca
    22801 acgccaagtt cacgccaggg gagcgattgc agtgaacgtt catgcaatac gcggatggcg
    22861 tcatcaccaa caaaaagttg atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg
    22921 ctggtgtcat atttcaacaa atgcgccatg cccgcagcat ccgccagttc gttgattgcc
    22981 accacggtaa tttccgcccg gcgtccggat tcatacaaag cacgaaccac attacgcccg
    23041 atgcgaccga agccatttat cgctacgcgt acggtcatag atctcctgca aggttttccc
    23101 tgagcaaatt tgccagacag agtaatccag caaatcgtcc ggggaaacct tacctgtcgc
    23161 aaactgcgac tgattggtta attgtcgaac atttaatcga ctgaaacgct tcagctagga
    23221 taagcgaaac gtggaataaa aggaatgatt gtccagtcaa agaaagcaat tatctgactt
    23281 acgtcacgtt ttcagccccg cccctgtgga atttattcca ctttgcagaa tcacgaataa
    23341 gcgtagcaaa atcttttcgt tgtgccgtca caaaccctga cttatatcag aatatgactg
    23401 atgcagcgtc gatattatcc gcaatacgtc cgagtttcgg agtactccaa tgatgacatt
    23461 gccctaccca tattcttcct ccgcacgctt ttacgcactg cgcctcctcc ccgggcagga
    23521 agttttttcc cagctacacg cttttgcaca gcagcatcaa cttcatgccg cctggattgc
    23581 aggctgtacc ggtagcctga ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac
    23641 gctcctgaac ggtacattcg aagtgatctc actaaacggc acgctggaac agagtggtga
    23701 gcatcttcat ctctgcgttt ccgatccaca cggcacgatg cttggcggtc atatgatgcc
    23761 cggctgtacc gtgcggacca cgcttgaact ggtgatcggt agccttgagg aactggcgtt
    23821 tagccgccaa ccttgcgcgc tttctggtta cgacgagttg catatttccc ccgttaaata
    23881 actactttca aagaggtttt cttatggcgc gtagccactt ttccagtcag gcactggtgt
    23941 tgatcgttat atctatcgcc atcaatatga tcggcgggca actggccagc atggtcaaat
    24001 tacctatatt tctcgactcc atcggcacac ttattagcgc ggtactgctt ggtccggtga
    24061 tcggaatgct gaccggctta ctgaccaatt tactctgggg attgctgacc aacccgatcg
    24121 ccgccgcatt tgcacccgtc gcgatggtca tcggcctggt cgccggttgc ctggcgcgag
    24181 cgggttggtt tcgcaccctg ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg
    24241 taacggtcgt cgctgtaccg ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag
    24301 ccgatttgtt cgttgcctgg atgcactcga tggggcaaaa cctggtggag tcggtggcga
    24361 ttacggtgat aggcgcgaac ctggtcgata aaattcttac agcggtgatt gtctggcttt
    24421 tactgcgcca gctgccgatt cgcaccacgc gccattttcc ggcaatggct gccgtgcgct
    24481 aaatgcatcc atttacctca ttaacactgt gggcattggc ggcctgcacc acgcttatcc
    24541 tgcccgcgca gaccattttg ccgatctata gcgcggcgac ctttttctgc cttattgcgc
    24601 taaaggccac gcagcggcgg gcaaaatatg tcgtctggct gatgttctcg ctgggagccg
    24661 ggctgtggct ggtacacggt ggctggctac cagaatggct aagcggcacg ccacgctcgc
    24721 cagaacggtg gtcgcatgct attacgctct ggttgcggat tctggcgatt gtttcaacct
    24781 cacagttgtg gatgcagtac gtccccgtgc aatgttttat tcgcgctctg tttgcttctc
    24841 gcctgccgcc aggcgtcgct tatctgtttg ccgggccgtt attggtcgtt gagcagttaa
    24901 aacggcaatt agcgattatt catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg
    24961 gctggtatca gcgtttacgt gcgatgcccg cgttaattat tccgctaaca cacaatgcgc
    25021 ttaacgatct ggctgtccgt ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc
    25081 gacgaacaac gttgtgggca ccagccgata gcaccctaca acgcgtagcg cgttacacca
    25141 tgatcttgct gatgctgaca gaattcggag cgtggatatg gttacgttag aacagttccg
    25201 ttattgcccg acacactcaa cgcatcctcc gttctgttat gactttcact atgtcaaacc
    25261 ggggatggtc gccatctttg gtgacaacgg cagtggcaaa agtacgctgg cacagctgat
    25321 ggcgggctgg tatccggact ttctgccagg agagataaca ggcacgggga cgctcctcgg
    25381 tacgcccatt gggcatctcc cgcttaatga acaatccgcc accatccagc tcgtccagca
    25441 atcgccctat ttgcagctct ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg
    25501 gccagaaaat ctgtgtcttg ctgaagcaga gattatggcg cggattgatg ccgccctgac
    25561 tctgaccgag tgccagccgt tgcgccatcg tcacccggca acactctccg gtggagagac
    25621 acagcgcgtg gtcatcgcct gcgccatcgc gatgcagcca aagctcttaa ttcttgatga
    25681 agcctttagc cgcctgactc cgcaggccag cgaaatgctg ttgcagcgct tacaacactg
    25741 ggcgtttgaa cgtggttcgc tgattattct ttttgaacgt catcgcacac ctttcctgaa
    25801 ctactgccag caagcgtggc aattgcaaaa cggagcgctc cagccactat gttaacgtta
    25861 aataagatat cttatcgctg gcctggcgcg gcaacggatt gtctttgcga catttctctg
    25921 caactcaaac aaggtgaatg gttggcgctg accggcgaca atggcgcggg aaaatcgacg
    25981 ctgttacgag taatggcagg tcttcttacg cccacagccg gtacggttat gctgcaacaa
    26041 caggcgatga aaaacctgaa aaaccgccag cgcgcggcaa aggttggcgt actgtttcag
    26101 gaagcggaaa accaactttt tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa
    26161 ctgcaaaaat gcccggcgga tgaaatcact caacgtaccc atgctgccct gcaatgttgt
    26221 cagttagcag acactgccaa ctcgcacccg ttggatctgc atagtgcgca gcgacggatg
    26281 gtcgccgtcg cctgcctgga agcactctct ccgccactat tgctgctgga cgagccgagt
    26341 cgggattttg acgaaaattg gttgagcgtg tttgaaagct ggctggagaa gtgcggccag
    26401 cgaggcacga gcgtcgtagc aatcagtcat gacgccgcat ttacccggcg tcatttctct
    26461 cgtgtggtgc gactggaaga tggtctaatc aggaacgtta atccaccaga cgatattcac
    26521 ccgtagcggt catcattaag gtcaaatttg caggcagact ctcttccagc ccccggcgaa
    26581 cattcggccc gtcggtacgg tcataaaaag cttctgcgtc tgccagcgat acccctcccg
    26641 ccagcttgcg ccctaccagt cgttcccgca acgcagtagc tggcgcgttg ataaagatcg
    26701 agaaatcaca aaattgcgca agctggcacc acttctcgtc gtccagcaac aaccagtttc
    26761 cttcgacgat gacgagtggt gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt
    26821 gtcgatcata ctgcggccac gtacaatccc cttccactac ctggcacaga ttttccgcca
    26881 gtttcgcaac gtcaaatgtc tctggtgcgc ctttgaaggg gcgcaattga tgcgcatcca
    26941 gccagctatt gtaatggtga aaaccatcca tcgggagcgt ctggatagcg ggtaattcag
    27001 ggtcttgttg cgcgagatat tcccagaagg tagttaaggt agatttaccc gtccccggcg
    27061 gagcgcaaag aaaaaccacc gtacgtcgct gcggacttac cgtctgtaag gccgccagca
    27121 tacgcagcaa cggtttatgc acgcgttcta tctcttcatc atggtactgc gcttgcacgt
    27181 tcagcccatt caccgttaat tcaattttca cggcgttaat tcctttaagg tggtcgttac
    27241 tgccagcgtc agcgccgttt tgaccattcc gctaaaacgc gcctctgacc agacggcgaa
    27301 atcggcgaac ttcatcgctt ttaacgcgtc atagagcgtc gcattgcagg ttatgctatt
    27361 gacgttgctg ataaaatccc aggtcatgtc atcggcaaat ccgggcgttt catcctgttc
    27421 gttcaggtaa tgccagtact gggaaaagtg cgtgatatcg gcatacagcc atttatccat
    27481 tttccccagc gcataaatca aacgcagcgc aacatcaata tcgtcaagcg ggccgctttg
    27541 ggcgagtaac ggcttcactg catattcgac gatctcttca tcgttatcga caaacagcga
    27601 cggcagaaac tggcgtatcc cctggagcaa aatagaatgc gcagttttca taaatgaaaa
    27661 taaattgtcc tgtgcatcca gttgctcaag cacatcatct tctgtcagcg tcgccattgg
    27721 tcttccgata gatcaccatc aatgcaggga gtctattata ttacacgttt ggtccgcgcc
    27781 gccgatcagt aatgtctgcg tccgcacccc attcgccgtc tgttggatac cgttcaccaa
    27841 ctcgccgccc gtcacgcccg tagcgctaaa gaggatatcg ttccccctca ccagttcatc
    27901 gagcgagtac acacggttaa cgtcaacgcc cattgcctta caacgcttgc gctcctgctc
    27961 tgcaatctgc cgattttccg tgtaatcccc tttagcctga caaaagtcaa tcagctcggc
    28021 ctgcatatcc ccacctaatg ctttaacggc acaggcagaa atcacgcctt ctggcgcacc
    28081 gccgatggtg tacatcacgt cgtaaggatt atcctgccag caggtcagca cgctggcggc
    28141 gacatcgcca tccggcagag cgaaaacctt cacgcccagc tgtgttgctt cttcaatcgc
    28201 ggcactcagg cgcggtttat ccagcgtaac catgcgcagc ttgtccagcg gtttacctaa
    28261 cgcccgcgcc acgttacgca agttgtccgc cagaggcaaa gacagatcaa tcgccccggc
    28321 agccagtcga ttaacaacca gctttttcat atacatatcg ggcgcatgta acaggctatc
    28381 tcgaggggcg aaagccatga ccgccagcgc attgctctgc cccatcgcta ccatccgcgt
    28441 gccttcaatc gggtcaaccg cgatatcgac ttctggccca tcgcctttgc ccacctcttc
    28501 gccaatccac agcatcggcg catggtcaat ttctccttcg ccgataacca ctcgtccacg
    28561 aaaagcgacg tcgtttaatg cctggcgcat cgcggtgacc gccaggccat caattttatt
    28621 tttgtcgcca catccggttt gcggccaggc agccaacgct gcctgttccg taacgcgaaa
    28681 taatggccac gccagggaca tcattgcgcg gcctccccga tatcaacgcc aaattgtgcc
    28741 agcagatatt tctcggcctg ttcattccag atgccgtgcg tttcctcaac cagccgcgcc
    28801 aactctttaa acagcggatc ggttttgcct ttttcggcaa aatcggcaat ggcagtgagc
    28861 ggcatggtca cgccgttata aatgagtttt ttaccgccag ggatatccgg cagattgagc
    28921 acggtatctg gcaccgcatc cagcccaccg atatgcgtga ccataaagga cggctgcaac
    28981 tgccccgtgg cgcttaacgc aatcgcctct ttcatgtcgt ccgttgaacc gccggatgta
    29041 ccgactacgt gcgtgctatt gtaatggacg ttgtagaaat taaacggcac tttgaagttt
    29101 ttatccgtcg gcccggcaaa gaagttcaga cagccatctt ccgccagtaa ttcgtcagcc
    29161 atctcaacga cagcaggcac cgccgcataa acaaaaatgt catcgaaccc gacatctcct
    29221 gtcagcgccc gcagcgtctg gaccggatcg ctcatccctt tggtattcac atacaccagc
    29281 tcaatgcctt tactggccgc cagatccacc ggcagcagct tctgcacctg cgccaggcgt
    29341 ttgtcgtcga tatcgaccac caccacccgt gacggttgta tgccaccgtt aatggcgtaa
    29401 tcgatagcgc caatgcccat cggacccgca cacgccagca gtgcaatatt gccgccaggt
    29461 ttgacgccca tgcgatgctc ataaacatat tgcgtggtgt gataattggc atgataagca
    29521 ccaataatgc agcacatagg ctctgccagc gacgccgcag caaaataaga gccgtgatac
    29581 ggtaatacgc agcccaaatt aatggcgatt tcgggaataa tcatataggt ggcgttaccg
    29641 ccaaaatatt cgtagctgta gcctgctgaa tatccgcttg gtaaccccat cgccggttgc
    29701 aatacaaaac gctggccttt tttatatttg ccagtgagat ttttacccac ttcgacaata
    29761 accccggcac attcatgccc ggtaatgacc ggatgatttt ctaaatcgtc gggtacgcgt
    29821 ttatgttcac taccgagtaa cgccgctttc caggtcgata aacagacgct gtcagaaatt
    29881 acactcacta acagttcatt atcggtaatt tctggcagtt caaattcgcg cagacggaca
    29941 tcccgcttgc cataaatagc agcaactttg gttttcattt ttacctctgt aatcagtgtt
    30001 tatgttcggc ggttaattga ttaaataaag tgtcgagttt tggatcgcca atatagttat
    30061 taatcaatat cagcggttta tccgtcacac gtttcacgcg cccttccaga ctggcgtgag
    30121 tgacgacgat atcggcatcc gcaggcacgt tttctatcgc gtaatgtttt acttctattg
    30181 ccagccccgc tttttccagg cgtttacgga aggtggtcgc gcccatcgca ctggagccca
    30241 tccccgcatc gcaaacaaag gcgatacgct taaccctgct taacgagaat gcaccctctt
    30301 gtttcatcgc cttaaccgca ttggctgact gagcaaactc atcttcgctc tccgtttcca
    30361 ccgttttttc catcttcagt atcagcgaag taatagcaaa ggacaccagg gtacctaccg
    30421 taacaccggc aattgtcgcc aggaacgagc ctttcggcgt cagtgccagg taagcaaaga
    30481 tagaccccgg acttgggcca gccaccagac caccgtccag taagttaaac atccaggtac
    30541 cagacatacc gcccgcaatc atggcaataa tagtcagcgg cttcatcagc acatatggga
    30601 aatacagttc gtggatccca ccgaggaagt gaataatcat cgccccgggc gcagaacgtt
    30661 tactcatccc tttaccaaac aaggtaaacg ccagcagcag ccccaggccc ggacctgggt
    30721 tagaggccac cataaagaag atggatttac cgttaaccga agcctgttgc attcccagcg
    30781 gataatagac gccctgatcg atcgcattat taaggaaaag cactttcgcc ggttcattga
    30841 tgactgacag cagaggcaga taacccgcat gtaccagcgc ctcgatacac tctttgacga
    30901 aagtattggc aattaacacc gccgggccga tcacttcaaa acccagcaga caaaggagca
    30961 tccccgcgat acctaatgag aagttattga taaccatctc aaaaccggca ggtatgcgtt
    31021 tttccagtgc cttatcgaca tattttatga ccaacccacc gagcggcccc atgatcattg
    31081 agccaaggaa catcgggatc tctgcgccaa cgatcacacc aatagtacct attccgccca
    31141 tgaccgcccc gcgtttaccg ccgaccagat gaccacctgt agaaccaatc atcacgggca
    31201 ataaataggt aatcatcggg ccgacaattt tggcgaaatg ttcgttaggc agccaaccgg
    31261 tgggaataaa taacgcagta ataaaacccc aggcaataaa agcaccaata ttggggatga
    31321 ccattgcagt caaaaagccc ccaaaagcct ggacctttgc acgagcagac ttgttttcca
    31381 taatattatc ctgtagagga gagagtaatt atccgcggct gataatgtcc gctaattgtt
    31441 tttctgatga tgctgttaag agttgttcga gtatttcttc ttcgcacagt aattcactta
    31501 acgcctgaat agcgccaatg tgcgaatcgg catcggcagc agagagtccg atgagtaatt
    31561 taattggttc gtcattcccc ggaaaataaa caccttgttc aagtaatgtc aaagacatcc
    31621 cggttttaag cgccccacat tccggtcgcg catgaggcat tgccacgcct ggtgcgagaa
    31681 tataataagg gccattgtta atggtggaat ctttaattgc ctgaatgtaa ttctcgctga
    31741 tatagttttt atccagcaat gataccatcg agaaatcgat agcttcctgc caatcttttg
    31801 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
    31861 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
    31921 aaagcaattg agcagggaaa ccgatgtttt gaaaacagtg aattattaat ttcttatata
    31981 acattgagtt atagatataa caacaaatgt caatacgcat atcgtgatgc atattacata
    32041 attgatattt attgcatata aatatttgtg atctacaaca cgctttatct attgcttgtc
    32101 cgcaaacgga catatcaagg taataaaaaa ggtcgccgaa gcgacctttt tacccgaaat
    32161 gctaattaca gcagttcttt tgctttcgca acaacgttat caacagtgaa gccgaactct
    32221 tcaaacagca gctctgccgg agcagattca ccgaaggtgg tcataccgac gatagcaccg
    32281 ttcaggccaa catacttgta ccagtagtca gcaatacccg cttctacagc aacgcgtgca
    32341 gtaaccgctt tcggcagtac ggattcacgg taagcagcat cctgcttgtc aaatgcgtcg
    32401 gtagacggca tggacaccac gcgcgctttc acgccttcgg cagtcagttt ttcgtaggca
    32461 gcaacagcca gttcaacttc tgaaccggta gcgatgaaaa tcagttccgg ctgaccggcg
    32521 cagtctttca gcacataacc accgcgcgcg atgtttgcca gttgctcttc agttcgttcc
    32581 tgctgcgcca ggttctgacg ggagaggatc agtgcggtcg ggccgtcctg acgctcaaca
    32641 ccgtatttcc acgcgaccgc ggattcaacc tggtcacacg gacgccatgt agacatgttc
    32701 ggggttacgc gcagagaagc gacctgctct accggctggt gagtcgggcc atcttcgccc
    32761 agaccgatgg agtcgtgggt gtaaaccatc acctgacgct gtttcatcag cgcagccata
    32821 cgtacggcgt tacgtgcgta ttccacgaac atcaggaagg tggaggtgta cggcaggaaa
    32881 ccaccgtgca gggagatacc gttagcaatc gcggtcatac cgaactcgcg aacaccgtag
    32941 tggatgtagt tacccgcagc atcttcgttg attgctttag aaccagacca cagggtcagg
    33001 ttagacggtg ccaggtcagc agaaccgccg aggaattccg gcaacagcgg accgaaagct
    33061 tcgatagcat tctgagacgc tttacggctg gcgattttcg ccgggttagc ctgcagttta
    33121 gcaatgaact cttttgcttt cgcgtcgaag tcagacggca tttcgccttt catacggcgg
    33181 gtaaattcag cggcttcctg cggataagct ttcgcgtaag cagcgaattt ctcgttccat
    33241 gcggattctt tcgcctggcc tgcttctttc gcatcccact gagcatagat ttcagacggg
    33301 atttcgaacg gtgcatattt ccagcccagc tgttcgcggg tcagggcaat ttcagcgtcg
    33361 cccagcggcg caccgtggga gtcgtgagta ccggctttgt tcggggaacc gaaaccgatg
    33421 atggttttgc acatcagcag ggacggtttg tcagtcactg cgcgcgcttc ttctactgcg
    33481 cgtttgatgg atgccgcgtc atgaccgtcg atgtcgcgaa taacgtgcca gccgtaagct
    33541 tcgaaacgca ttgcggtgtc gtcggtgaac cagccttcaa cgtgaccatc aatagagata
    33601 ccgttgtcat cgtagaacgc aatcagttta cccagcttca gcgtacccgc cagagagcaa
    33661 acttcgtggg agatgccttc catcatgcag ccgtcgccca tgaaggcgta ggtgtagtgg
    33721 tcgacaatgt cgtggcccgg acggttaaac tgcgccgcca gcgttttttc tgcaatcgcc
    33781 atacctactg cgttggcaat accctgcccc agcggaccgg tggtggtttc cacaccagcg
    33841 gtgtaaccca cttccgggtg gcccggagtt ttagagtgca gctgacggaa gtttttcagt
    33901 tcttccatcg gcagatcgta accggtgagg tgcagcaggc tgtagatcag catggagccg
    33961 tggccgttgg acagcacgaa gcggtcacgg tcagcccagg acggattctg cgggttgtgt
    34021 ttcaggaaat cacgccacag gacttcggca atgtcagcca tacccatagg ggcacccggg
    34081 tgaccggatt tggctttctg tactgcgtcc atgctcagcg cacgaatagc attggcaagc
    34141 tctttacgtg aggacatttt gactccagat cggatgatga agggcacgcc cttaacgact
    34201 tgacgacagc gcgttttggg ctacgccgga aaatttgcca acaatttacc gcaagccgcg
    34261 cgtcatgtac atggaacatc cttttgccgc ttcagaaatc tctggatcat gctcgcatgt
    34321 tgcgcaatct actcgcccgt ccgctgcgct tttccttata ctgagactga gcgtcgattc
    34381 acctgcaaac ggcgcatttt tagaataatc ctgaccttgt gcggaagaga aaacatgaaa
    34441 attcgcgcct tattggtagc aatgagcgtg gcaacggtac tgactggttg ccagaatatg
    34501 gactccaacg gactgctctc atcaggagcg gaagcttttc aggcttacag tttgagtgat
    34561 gcgcaggtga aaaccctgag cgatcaggca tgtcaggaga tggacagcaa ggcgacgatt
    34621 gcgccagcca atagcgaata cgctaaacgt ctgacaacta ttgccaatgc gctaggcaac
    34681 aatatcaacg gtcagccggt gaattacaaa gtgtatatgg cgaaggatgt gaacgccttt
    34741 gcaatggcta acggctgtat ccgcgtctat agcgggctga tggatatgat gacggataac
    34801 gaagtcgaag cggtgatcgg tcacgaaatg gggcacgtgg cgttaggcca tgtgaaaaaa
    34861 ggaatgcagg tggcacttgg tacaaatgcc gtgcgagtag ctgcggcctc tgcgggcggg
    34921 attgtcggaa gtttatctca atcacaactt ggtgatctgg gcgagaaatt agtcaattcg
    34981 caattctccc agcgccagga agcagaagcc gatgattatt cttacgatct tctgcgccaa
    35041 cgcggcatca gcccggcagg tcttgccacc agctttgaaa aactggcaaa actggaagaa
    35101 ggtcgccaaa gctcaatgtt tgacgaccat cctgcatccg ccgaacgcgc ccagcatatt
    35161 cgcgatcgta tgagcgcgga tggggttaag taagtcagac ataccgatct acaacataat
    35221 tgtaggtcgg taacgcctga atccccgatc agagtagtga tttcacttta tttagcaaaa
    35281 cggctacatc atccggtcgg gtatatcccg tatcagggtc tataatcgag ctgtaaacat
    35341 gtggcataat gcgcggcacc cccgcctcca gacaagtttt cagaatcaca ctgaagttat
    35401 ccaaatcaat cccgccagtc ggctcaatca gcgtcatacc attgcgcgcc gccgtggtcg
    35461 ccagcgcgta tagctccgga agcgacctct cgccgcccat cggaaagaac ttcgccgcgt
    35521 gtgcgcccat atctagcatc attcgcaccg ccgtatcgca ggagacgcgg gcaggcgtac
    35581 cctggctgct gctaacgccg gtggaaatca gcacttcgcc cggcgtcccc gtcgggctaa
    35641 ccagcgcgtt gacatgggtc tgctcaccgc ccgtagccgc aagcgcgcct gccgcgaaac
    35701 cacaaccggt aaaggtctgg ttaacgtgtg ctggatgaat ctgcgccgca atcatcgcag
    35761 ctttgtaata ctgcgccggg tcacccgccc ccagacctac tgaaatggac ggtatttccg
    35821 ccatccaacg tttaacttcc tggatcccct catccaccga ggagaactgc gcggaaagca
    35881 cgccaatcac tgcgtggcct tcggcggcgt caaagatttc acgggcattg gcgatatctt
    35941 tcgccagcac attaattgcc acgcgattac gatagaaatt aatcttttgc atgatctgca
    36001 atctccttca gtcgcgccac gataagtgcc atttcccctt ccgccaccgt acgcggatca
    36061 aggctaaaaa cgccctggtg gaggttatac cgccgtgcat aaatcgcgat atcgccacca
    36121 cgcagttgcg cttcgacgac gcgagcgtca actcccaatt cctgcgcatt tactcgaata
    36181 cgaatacgcc agattgcacg cccggcttca tcctgctcaa tatcagccgt taacccgtga
    36241 atcgcagaaa tcgcttcaac cactggctgg agctgttccg cggtgatgac tgcctgcccc
    36301 tggtgataat tctccagcgc gtaaaccagc ccaaccatgt tctccttgcc gattttcatc
    36361 gccctggcaa tgccgtgatg ctgcgctttg caggctgcaa tccagatttt tttaccagtg
    36421 ataaacccgg aggtcggcgc gttaaaggct tttgcgccgc tgtagaccac catatccgct
    36481 ccgctcacca cccaggcacg taaatcctct tcggcggcgg catcgacaat cagcggcagg
    36541 ttgtacatct gcgcgacatg tgcgaagtcc tcgatgttca acattccctt ctgcacgcag
    36601 tgatgcgact tcacatacag cagcgcggcg gtcgcgtcgg taatggcact ctccagctgc
    36661 cagagagccg ccaggttact tgaacccacc tccacaacgc gcccgccacc taagcggatc
    36721 gcgctgagga tcgatgcacc gtaatcgacg ttatggccgc gcagcatcac cacttcattg
    36781 gccatgccgg agctatccgg catgagcgcc acccgggcct tgtcgccgcg tgtaatggcg
    36841 gcggcgacag cgatggcgat ccctgctgag gcacaggagg taacgtagct atcctccgcc
    36901 ccggtaaagc gagagaccag ctcgccggtt ttgtccacca gctggtcaat ctccacgaaa
    36961 gaggatgccg cgcgcgcggt ggcctgcatc acctccggcg cgacgctgga gacgccgaga
    37021 atcgtcatct tgccgcaggc gttaatcacc tgtttcagtc ccagttgttg atagatattc
    37081 tgcgtcatgg cttacagcac tcccagcagc gagcagacca cgctcaacgc cacaatcgac
    37141 agcagaatcg tggtgtaacg cggacccttt ttcaccaaat agaagtagat ggcgaacact
    37201 gccgccagcg gcagcaggcc gggcgcgatg gagtccagaa tctgctgtac caccacttca
    37261 gagcctttca gtgcgctaat ttttaaaggt gaggttatct tgacaaagct cgcggatagc
    37321 gcgcccatca tgataagccc cagtacgtta gcgccgtaga taagctcctt gatgcgccct
    37381 ccctgcagca gactgataat cgaatcgcgc cccagcgtat agcccttgtg caccatgccg
    37441 tagctgattg ccagcgtaat cgccgggtag agaatcagcg gcaaaatacc gcccatcgcg
    37501 ctgccattgg cggcgaacgg gataaaaata gcaatcaaca gcggcatcac cgccgcccag
    37561 atgatggagt cgcccatccc ggccagcggc cccatcaggc cggttttaat cccggtgata
    37621 gaggcatcgt taatcgcctc gccgcgggtc ttttgctctt ccatggcgat ggagatcccc
    37681 tggatcaccg cgccaaacgt ctgctctgag ttgaagaagt tcagatggcg ttttaacgcc
    37741 tccacctgct cctccttttg cggatagagc tttttgataa tcggcgtcat cgaggcgcag
    37801 aaaatcaggc tctgtaaacg ctcataggag ctggaaactt ccgcgcccag ccagtagata
    37861 aaccacgctt tggtgatatc cgctttggtc agcgccccgg actcccgcgc gcgctcaacc
    37921 agttcatgct gcattacgtc gtcgctcatt atgccgctcc ttcatttttc gccaggcctt
    37981 taatcaggaa ggccacgcag gtgccgaaaa tcgccatcgc catgatgtcc accttgaggt
    38041 acagcaccgc gaagaatccg ccgataaacc acggcagcag gctcttttta ccaatcacca
    38101 taatggtgat ggcgaagccc agcgccggga gaatcccgcc catgatttca aacgagtggg
    38161 tcagccagtg cggcatcagc ttgaggaaac tttccactac gttctggccg aagtagttgg
    38221 cggcgaacac cactgggaaa cgtagtacca gccccagcag cgccgggtag agaaaggcgc
    38281 agcgcataat ggctgacata ttggccgttt ccgcgtgttt atcggccata tgcacccagg
    38341 ccgcatttag cgtgcgacgc aactggtcga ggaatacgcc gataacgcca aatggaatcg
    38401 ccagtgcaat cgccaggttc gggtccatat tggccttaac ggcgatagga atggcgatac
    38461 aggccgccag cgccgggtct gacggcacat tgccgcctgg cgttgaggtc acacccagat
    38521 agaccaactg catccccgcg ccgataatca tcgccgcagg catattgccg agcagcaggc
    38581 cgacaaagac cgccaccacc actggctgta acagcatggc ggagaaggta tagccaaggc
    38641 gcaaccgggc aaaccagtaa tacagcccca tcaggcttgc aaaaagtaag gtatccataa
    38701 tttgttatcc catgttcaga gggtcagaat tttttcagga tatcgtccag cgactgcggt
    38761 ttatcttccg ggatggtctg gaaaacagcc ttaatgccgc gacttttcaa atcgttaaga
    38821 atgccaacgt ctttttcatc cagcgttatg ttctggaaca ccgctttacg gtttggcccg
    38881 ccgcctaaac cgccaacctg aatatcagcc acgtcaaagc cctgctgcac cgcttcctga
    38941 atcgccgcca gcgacgggaa cagcaccagc acgttgccgt cgcccagctg gttctctttc
    39001 cacgcggccg cgaagctctg gttgctaaag cagtccactt tgatattcgg cggcgcggcc
    39061 atcaggtaga tatttttcat aaacggatcg gcgtccagct cgtcgctgac caccgcaata
    39121 cggttggcct gagactgccc tacccacttc gtcaccacct gtccgtggat aagacggctg
    39181 tcaatgcgac ataacacgat gtttgccata cgattttcct tactgttgta attgacgaat
    39241 atgggagacg acgtccaggc aactgttgcg cccagcttcc accagatcgg ccacgcaggc
    39301 ggtcagtggg ccatgttcgc gcttgtccag cgcctccagc agcagcgagg cattaagccc
    39361 ggagatcacc gccaccggat aatcggcgct cagccgcgcc gccacgttag aggtggtgcc
    39421 gccgataaaa tcggtcacga taagcgaacc ttccggcatg gttttcacca cggcttcgac
    39481 gcgctggtaa aactcgccca gcgtatcgac gggcatcagc gcgatttccg tcacgccttc
    39541 gatttcgccc atcaccatgc gcagactgtt gcagagctgc agcccccagc cgccgtgagt
    39601 tagcagcaga atttcaggta acggatgaat aggtacagtt gtggtcaaaa tagcctcctt
    39661 tgctccagtg tttacatttc atcaggagca aaggacgtgc cagaatttgt gtcacgcaca
    39721 gaggaaatga gattggatgg ggcgttaagc cccgtaaacc aggggatcag ctgtagagca
    39781 gttcgtagat ataaaaatat tctgcgtccg ataaacggat agcgtaagcc tcttcaatcg
    39841 gcaaaaagga ggatttgatg acgctaaagg cacgagcgtc cagatcaagc cggttttcta
    39901 gcgccatctg taacggtttg cggttaatca cgatacgctc gaccatgcag cagcagtgga
    39961 tcagaaaacg tagtgtcacc tggcggctcg gtttcaggga gagcgtgttg gttaagtagt
    40021 tcagaacatc ctcgatctct ttcagaattc gctgcgggtt gagcaccgaa atatggttga
    40081 tgatgctctc catggtcagc gcgctgataa agcgcatggc gctgcgctcc atctccagtc
    40141 ggcgctcgct gcttgagaga tccggcgtca gcaggctgag caccagctcc ggcccttgct
    40201 cggaaaacag ttcctccagc gagataaacg gtatatccgg cagtcccggc tggaaggtgc
    40261 cgacgatccc tgccagccgt tcactggcat taagcgcctg ctgaatacgc tccaggctgc
    40321 gcacctcgtt atagtcgaga atcaccatac gcgtgtcctg cgacatcagc tcgccaaagc
    40381 tctcctccag cacctttttg atcttttccg ccgtccccat tccggttata catgagataa
    40441 ccagcacttt gccaccgctc tcttgctgcg gcgtgcagag ccgacaaggg atatttttac
    40501 tctgcataag cacagccaat tgcgatagat cgccagtttc gtagcttaaa tccagcccaa
    40561 cctccagcag gctggtcaga gtgatatttg gcaacaacag aacgtcaatc tggaacagct
    40621 tgctgatggt gctgccaaaa tggaccagcg atccgatatc caccatcagt atcagccgct
    40681 gccagcaccg gtcctgaatc atccgggtca gggtttccag ggtgtcatgc accgactgct
    40741 caaatggcat atcaattgcg tagaacagat cgcgctccag cacccggttc acatattgcg
    40801 cctggctcgt tgcggtggtc gcaccgtgag cgattagaat cacgccgcaa tccgggctgg
    40861 tgtcgatgcg ctggcggtag tggcgacact ctttcaggaa caggcacagc cagaccacct
    40921 ccgtcgccgg gcactgaata tgcaacaact cattgatttt gcggcacaga agcgtggcat
    40981 tgtcatactc atctttacag cgatccagaa tcaggctgga tgaatagagt tgaggtataa
    41041 gccctcgccg cacatagcca atcagcgcaa gaaagtgttt acgtaacggg ttcgccaggt
    41101 tttctggcaa tgtaaatcct aatacctgct ccacgtaacc gatcagcagc gtcacacgct
    41161 cctcaatttg atcgccatag cgcggtggat gcgccgcact atggcggcta taaagaccat
    41221 attcaaaaat cgaactgagt ttgtttttca ggatagccag cgtttctgcg ggcggcacgt
    41281 tactgttgcg cagattgaca tattcacgag tcaggaaact gtaaaacagg tcgctctctt
    41341 ctatttccgc gctggtcgcc aacgaatttt ttaacgcggg caacgtacgc gcatccacgt
    41401 tcagacgatc tttaccgcca aacaacgcat cgaccagcag acgctgctct ggcgtggtat
    41461 taaacgacat ctccgccagt cgtttatcta actctagcgt atcattacgc tcggtcattc
    41521 ccgacgccca cgcctgcgcg cacaagaact gaatatcact tttaagctgg ccgatattgc
    41581 cttccagggg tttacttaac aaccaatgca agagcgtttt atcaaggctt atggtacgtt
    41641 caattttacg actttcacgc tgaaagaagc ttacaatcaa ttcgagctgc tcctcgaccg
    41701 aacgctggcg aatacccggt aagtcaatac atacctggat acgccgctgg aaggtacgca
    41761 atagtgccga gttgaccgac tctgtagtgg cgcaaataag gcgcaccgaa attgaatatg
    41821 ttggcccact tgaaccaagt gggcggtatt cacctttatc gagaatagaa aagagcttct
    41881 cttgtccttc gtacgggagg cgatgcactt catccagaag taaataacct ccgtcagctt
    41941 gctccaccaa tcccgtttta ttctcacttg ccccggtaaa tgccccctta tggtgaccaa
    42001 acagatgtga ggaaagcaac tccggattat gagcatattc ggcacagtta aagtaaacca
    42061 acggaggtgg cgaacctatg gtgtgcttgc aagcgaagca atgcattaac tcagcaaaga
    42121 atgttttgcc aacaccagat gcaccggtta acagcacatg tagtccgtgg ggataaagca
    42181 ccgctgcgcg gcctttttct accgcatcac ggagactacg gtcgtatccg ataagtccgg
    42241 aaaacggatc atcatctgta tatttttctt tctttggcaa taaactagcc actgaatgaa
    42301 ctatgcattc gcaatcatcc agttttcgat taatgagcaa ttctagtgcg tgacgatgta
    42361 gaaaaaaaac aggacgtccc tgacttttta ccaccaggcc ttcattccat aattgattaa
    42421 gatctttact aaccgagttg cgtgccagcc caaggttaaa accaatcgcc tcagcagtaa
    42481 atgctttctc cttcgctaaa tcagacagtt ccagcccata cgtcagccgc tccagctcct
    42541 tgtgaataac atcaatccgt ctcatttctt taccgcttaa aaaacaatag ttgttcagaa
    42601 catacactat ttgtaaacgt aagaatgaga actatcggga agaattaatg ttccttgtgg
    42661 caatctggtt tctgaatatt cgtgttttaa atattactta tggtctgaaa caaccaggca
    42721 ggtgaacaga gctaagaaaa agtagcgtag aaatataaaa acatatatcg cactgatgaa
    42781 tgtttcccgg catgactatg cacaccggga aatatttagt aaattactcg ccctttttcg
    42841 ccgcctgaat atacagcatc tccagcgcca gcgtcgccgc agccagagca gtgatttccg
    42901 actgatcgaa tgccggagcc acttccacta cgtccatccc aacgatgttg agatctttca
    42961 ggccgcgtac cagtttaatg gcgcgatcgg aggtcagacc gccaattacc ggcgtaccgg
    43021 ttcccggcgc aaatgcagga tccaggcagt cgatatcaaa ggtcaggtaa accggcatat
    43081 cacccacaat ttgtttcacc tgggcgataa cgtcatccac gctgcgatcg ttcacctggc
    43141 aggcgtccag cacggtaaag ccgttgtctt tatcaaattc ggtacgaata ccaatctgca
    43201 cggaatgatt cggatcgatc agaccttctt tcggcgcggt atagaacatg gtgccgtggt
    43261 caaattcaca accgttcgcg taggtatcgg tgtgggcgtc aaagtgtacc agcgccattt
    43321 tgccgaaatg cttcgcatga gcacgcagca gcggcagcgt aacaaagtgg tcaccaccga
    43381 aggagagcat acgcttaccg gcagccagca gcttctcggc gtgcgcctgc agcttctcgc
    43441 tcatctcacg ggcatcgccg aaagcatata ccagatcacc gcagtccacg acgttcagac
    43501 gctcacgcat gtcgaaattc cacgggaagc ggttgtgttc ccaggccaga ttcgtcgaaa
    43561 cctgacggat agctgccgga ccgtggcgtc cccccgcacg accagaagtg gccatatcga
    43621 acggcacacc agtaatcacc cagtcagcat cgctgtcata cggctggaag ttcatcggca
    43681 ggcgtaaaaa accaaaggca ttggaaacca gtgagttatc gtattgatga cctaaggtgc
    43741 tcatggatgg actcctatta caaagtcgat ataaaaaaac ccttccgcgt cgttaggccc
    43801 gacgaggaag ggttggattt gcgaaacaca agtttgtgct aattatcgcc ttaaatgggc
    43861 gaggcatcaa gcgaggttgc tatttccgac acggtggcgg cgcatagcca ttgttatctg
    43921 ccggaaaggc ttgtccagag cacggcggca gcgcgttttg cgcctggcct tgtaattcaa
    43981 aggcggcaaa gcctattaca ccggtattac gcacatcgcc ttgcgccgaa ttggctgcgt
    44041 aggaatcagc aacgttggcg aactgaaaag ccgcttcggt atgtttatct ttgcggaatc
    44101 ctttaatcgc taaagaatct ccggcattca caatatagcc gttgttattc agcgaacctt
    44161 gcttgccgtt cagcacatcc aggccatcaa cagtagcaac gatctcgtag ttggtgttac
    44221 gactgtaatt acggacatat aattgataat tcattccgac tctggcatgg acaatatagc
    44281 gtggttcatt attggatgaa tcataaacac gcgtaatagg tagagaatta aaattaccat
    44341 cgcgaatagc atattccagt tcgttaatac gaatgctata aacgcggtca tagccggaag
    44401 gaacattagt cgaataacta ataacgatca tctctgccgg atcgcgccag gctcgctcag
    44461 cagatacgcc ctgaaccgat gagtgcacat ccccacccca ggcagtgccg acattgcccg
    44521 atgcactgct attatactgc gcattactgg aacaaccggc cagcagcacg attaatgcca
    44581 ccaacgcgct acgtactttc cattttttca taaccgttcc ctctgcattt aattaatgca
    44641 gcgattgtag ggagattatt taataagaaa agcagcgaga ttatttttaa gaatatataa
    44701 aaagaaagcg ggccgattta cgtaaataaa tcgacccgcc agcggaataa tcttaattat
    44761 tcatcttcaa gataggtata accgtacaaa cctgcctcga actcttcaag gaactgttgt
    44821 tgcagttcag catcaagatc ggttttcttc acttgatcgc ggaactgggt taacagcgtt
    44881 ttcggatcga gctgtacata ttgcagcatg tccgccacgg tatcgccttc gtcagacagt
    44941 tctacttcta cgctaccgtc agggaacacg aacacgtcaa ccgcttcggt atcaccgaac
    45001 aggttgtgca tattgccaag gatctcctga tatgcgccga ccataaagaa accgagcatc
    45061 ggcggattct ctggatcgta ctccggcatt ggcattgtcg tggcaatacc atcaccatca
    45121 atatagtggt cgatagcacc gtcagagtca caggtaatat ccagcagcac tgcgcgacgt
    45181 tccggcactt gatccagccc ttccagcggc agaaccggga acaactggtc gatcccccat
    45241 gcatccggca ttgactggaa cagcgagaaa ttgacgtaca ttttgtccgc catacgttcc
    45301 tgcagttcgt cgattatcgg acgatgcgca cggttttgcg gatccagctg cttttgcact
    45361 tcatggcaca tgctcagata gagttgttct gcccatgcac gttcttgcag gctaaaggtg
    45421 ccggaagagt agccgatatg aatgtcgtgc agatccatct ggctgtcgtg taaccattca
    45481 cgcagagaac ggcgagttcc cggttcgtgc atttcctgcc aggtttccca catgctttgc
    45541 agcgcgcgcg gcgcatcttc tgcaggcgcg gtcggcaccg tgtattcgtt acgttccacg
    45601 ccgatgatat tagataccag cacggtgtga tgcgcagtca ccgcacgacc cgattcggtg
    45661 attaccgtcg gatgcggcag accgttttct tcacacgcat caccaatcgc ccagatgatg
    45721 ttgttggcat attcattgag gccgtagttc accgaacagt cggactgcga acgggtgcct
    45781 tcatagtcca cgcccagacc gccgccgacg tcgaagcact gaatattgac gcccagcttg
    45841 tgcagttcca catagaaacg cgcggattca cgaacgcctg tcgcgatatc gcgaatattc
    45901 gccatctgcg aaccgaggtg gaagtgcagc agttgcaggc tgtcgagacg cccggcttca
    45961 cgcagggttt caacaagttg cagtacctga gtcgcagcca ggccgaactt cgatttttcc
    46021 ccgccggagg actgccattt acccgagccc tgcgaagcca gacgtgcacg cacgcccaga
    46081 cgaggaacga cattcagacg ttctgcttca tccagcacaa tggcgatttc tgacatcttc
    46141 tcaatgacca ggtagacctt gtggcccatc ttctcgccaa ttaatgccag gcggatatat
    46201 tcgcggtctt tataaccgtt gcagacgatg acgctacggg tcatgccagc atgtgccagc
    46261 accgccatca actctgcttt cgagcctgct tccagtccca gcggttcgcc cgaatgaatc
    46321 agggactcaa tcacgcggcg gtgctggtta actttgatcg gataaacaag gaagtaatcg
    46381 ccgttatagc cgtaggattc ccgcgcacgt ttgaacgcgg cgttaatgga acgcaaacgg
    46441 tgctgcagga tctgtgggaa acagaacagt gcaggcagac gctgaccctg tgcttcacga
    46501 gttttcacta actgcgcgag atcgacgcga gcttccggga cgtccgggtc cgggcacacg
    46561 ctgatgtggc ccagctcgtt aacgtcatag tagttattgc cccaccaggc aatattgtaa
    46621 gtacgcagca tcttgctggc ttcctgggag ctcattgcaa cctcctgcat ggagcgtagt
    46681 acaccgtgtt cgcccgctga cgaaggcaaa cccatagaca tgtcgtcaga catagcgaac
    46741 ctcaaattat tttattaagt gtaaaacagt taacgactat cgcagcctgg aactgcgata
    46801 acaacccata agcacatgga ttttccagca gtgtatgctg acgctccgac tgcgcaaccg
    46861 gtttcttttt cataacatta ttaagcacat aaccgaacgt aagtgtgaca gttcggcgaa
    46921 accacgagaa aactcttgtt tttacaagag cgcccttgtt cagtcctcag taactgtaac
    46981 cagctcttga atcctgagaa gcgccgagat gggtataaca tcggcaggta tgcaaagcag
    47041 agatgcagag tgcggggaac gaatcttcac cagaacggtg agacaggtta agcagcagac
    47101 aacggttcat tatttcgtat cacctccacg gccgcctgtt aagacgaacc cacaagccaa
    47161 aactctgatt tcaacccggc tggaagtggc aacacgaaag aaacgtcgtg tgctttttat
    47221 ttaagccgcg cgccgcgttt tataccccac aatggcagaa aattgcaaaa gataaatacg
    47281 cagaatgccg gcattgtcag gaaaaatttc cagccacgtt tttaacagaa tgagacacga
    47341 ttcaaaaaaa agtggaaatc gggtgaagaa ttgacctaaa atagccatcc agatgttaat
    47401 ccatccatac cgattaacac tcagactgcc agtgttttta acttgcagag tcgtggtagg
    47461 atccgctacc acagaaaatc cacacaacag tttgagctaa ccaaattctc tttaggtgat
    47521 attaaatatg gcaaaacacc tttttacgtc cgagtccgtc tctgaagggc atcctgacaa
    47581 aattgctgac caaatttctg atgccgtttt agacgcgatc ctcgaacagg atccgaaagc
    47641 acgcgttgct tgcgaaacct acgtaaaaac cggcatggtt ttagttggcg gcgaaatcac
    47701 cactagcgcc tgggtagaca tcgaagagat cacccgtaac accgttcgcg aaattggcta
    47761 tgtacattcc gacatgggct ttgacgctaa ctcctgtgcg gttctgagcg ctatcggcaa
    47821 acagtctcct gacatcaacc agggcgttga ccgtgccgat ccgctggaac agggcgcggg
    47881 tgaccagggt ctgatgtttg gctacgcaac taatgaaacc gacgtgctga tgccagcacc
    47941 tatcacctat gcacaccgtc tggtacagcg tcaggctgaa gtgcgtaaaa acggcactct
    48001 gccgtggctg cgcccggacg cgaaaagcca ggtgactttc cagtatgacg acggcaaaat
    48061 cgttggtatc gatgctgtcg tgctttccac tcagcactct gaagagatcg accagaaatc
    48121 gctgcaagaa gcggtaatgg aagagatcat caagccgatt ctgcccgctg aatggctgac
    48181 ttctgccacc aaattcttca tcaacccgac cggtcgtttt gttatcggtg gcccgatggg
    48241 tgactgcggt ctgactggtc gtaaaattat cgttgatacc tacggcggca tggcacgtca
    48301 cggtggcgga gcattctctg gtaaagatcc atcaaaagtg gaccgttccg ctgcgtacgc
    48361 agcacgttat gttgcaaaaa acatcgttgc tgctggcctg gccgatcgtt gtgaaattca
    48421 ggtttcctac gcaatcggcg tggctgaacc gacctccatc atggtagaaa ctttcggtac
    48481 cgagaaagtg ccttctgaac aactgactct gctggtacgt gagttcttcg acctgcgccc
    48541 atacggtctg attcagatgc tggatctgct gcacccgatc tacaaagaaa ccgcagcata
    48601 cggtcacttt ggtcgtgaac atttcccgtg ggaaaaaacc gacaaagcgc agctgctgcg
    48661 cgatgctgcc ggtctgaagt aatctttctt cacctgcttt caaaggccag cctcgcgctg
    48721 gcctttttct tttacctcac cgcggtggat aggcattcac gccgcatccg gcaaaaagct
    48781 gcccgcacaa aaacatcatt cttcctcaac acgttttatc gcaaattatc atcattactg
    48841 aaaccgatta caccaataac accagccaaa tatttgtaat attttcatat tattattcag
    48901 ttttcacagt tgttacattt cttttcagta aagtcttaat tgcagataac agcgtttaat
    48961 ctatgatgat ataactcaat tattttcatg cacttaaacc ataactaaga taaatgttag
    49021 tgtaagcgat tacactgatg tgatttgctt cacatctttt tacgtcgtac tcacctatct
    49081 taattcacaa taaaaaataa ccatattgga gggcatcatg cctgacgcta aaaaacaggg
    49141 gcggtcaaac aaggcaatga cttttttcgt ctgcttcctt gccgctctgg cgggattact
    49201 ctttggcctg gatatcggtg taattgctgg cgcactgccg tttattgcag atgaattcca
    49261 gattacttcg cacacgcaag aatgggtcgt aagctccatg atgttcggtg cggcagtcgg
    49321 tgcggtgggc agcggctggc tctcctttaa actcgggcgc aaaaagagcc tgatgatcgg
    49381 cgcaattttg tttgttgccg gttcgctgtt ctctgcggct gcgccaaacg ttgaagtact
    49441 gattctttcc cgcgttctgc tggggctggc ggtgggtgtg gcctcttata ccgcaccact
    49501 gtacctgtca gaaattgcgc cggaaaaaat tcgcggcagt atgatctcga tgtatcagtt
    49561 gatgatcact atcgggatcc tcggtgctta tctttctgat accgccttca gctacaccgg
    49621 tgcatggcgc tggatgctgg gtgtgattat catcccggca attttgctgc tgattggtgt
    49681 cttcttcctg ccagacagcc cacgttggtt tgccgccaag cgccgttttg ttgatgccga
    49741 acgcgtgctg ctacgcctgc gtgacaccag cgcggaagcg aaacgcgaac tggatgaaat
    49801 ccgtgaaagt ttgcaggtta aacagagtgg ctgggcgctg tttaaagaga acagcaactt
    49861 ccgccgcgcg gtgttccttg gcgtactgtt gcaggtaatg cagcaattca ccgggatgaa
    49921 cgtcatcatg tattacgcgc cgaaaatctt cgaactggcg ggttatacca acactaccga
    49981 gcaaatgtgg gggaccgtga ttgtcggcct gaccaacgta cttgccacct ttatcgcaat
    50041 cggccttgtt gaccgctggg gacgtaaacc aacgctaacg ctgggcttcc tggtgatggc
    50101 tgctggcatg ggcgtactcg gtacaatgat gcatatcggt attcactctc cgtcagcgca
    50161 gtatttcgcc atcgccatgc tgctgatgtt tattgtcggt tttgccatga gtgccggtcc
    50221 gctgatttgg gtactgtgct ccgaaattca gccgctgaaa ggccgcgatt ttggcatcac
    50281 ctgctccacc gccaccaact ggattgccaa catgatcgtt ggcgcaacgt tcctgaccat
    50341 gctcaacacg ctgggtaacg ccaacacctt ctgggtgtat gcggctctga acgtactgtt
    50401 tatcctgctg acattgtggc tggtaccgga aaccaaacac gtttcgctgg aacatattga
    50461 acgtaatctg atgaaaggtc gtaaactgcg cgaaatcggc gctcacgatt aatctcctca
    50521 ggcttcctcc catcgcggag gaagccacct cttgcagtca tcttttcttc gctctatcct
    50581 ctgccgctat gaaaacatcc cgtctcccta tcgccatcca gcaggccgtt atgcgccgcc
    50641 tgcgggaaaa actcgcccag gccaacctga agctagggcg taactacccg gagccaaaac
    50701 tctcttacac ccagcgcgga acctccgccg gaacagcctg gctggaaagc tatgaaatcc
    50761 gcctcaatcc cgttttgctg ttggaaaaca gtgaagcttt tattgaagaa gtggtaccgc
    50821 acgaactggc acatttgctg gtatggaaac atttcggccg cgtagcgcca catggcaaag
    50881 agtggaagtg gatgatggaa agcgtgctgg gcgttcccgc ccgtcgtacg catcagttcg
    50941 aactgcaatc cgtgcgtcgc aacaccttcc cctaccgctg caagtgccag gagcatcagc
    51001 ttaccgtacg ccgccataat cgcgtagttc gtggcgaggc cgtctatcgc tgtgttcact
    51061 gcggtgaaca gctggttgcg aaataaccat ctgaactatc aggaactttc ctgatctggc
    51121 tgattgcata ccaaaacaac tttcgctacg ttgctggctc gttttaacac ggagtaagtg
    51181 atgtaccgtt atttgtctat tgctgcggtg gtactgagcg cagcattttc cggcccggcg
    51241 ttggccgaag gtatcaatag tttttctcag gcgaaagccg cggcggtaaa agtccacgct
    51301 gacgcgcccg gtacgtttta ttgcggatgt aaaattaact ggcagggcaa aaaaggcgtt
    51361 gttgatctgc aatcgtgcgg ctatcaggtg cgcaaaaatg aaaaccgagc cagccgcgta
    51421 gagtgggaac acgtcgttcc cgcctggcag ttcggtcacc agcgccagtg ctggcaggac
    51481 ggtggacgta aaaactgcgc taaagatccg gtctatcgca agatggaaag cgatatgcat
    51541 aacctgcaac cgtcagtcgg cgaggtgaat ggcgatcgcg gcaactttat gtacagccag
    51601 tggaatggcg gcgaaggcca gtacggtcaa tgcgccatga aggtcgattt caaagaaaaa
    51661 gctgccgaac caccagcgcg tgcacgcggt gccattgcac gcacctactt ctatatgcgc
    51721 gaccaataca acctgacact ctctcgccag caaacgcagc tgttcaacgc atggaacaag
    51781 atgtatccgg ttaccgactg ggagtgcgag cgcgatgaac gcatcgcgaa ggtgcaaggc
    51841 aatcataatc cgtatgtgca acgcgcttgc caggcgcgaa agagctaacc tacactagcg
    51901 ggactctttt tgttaacccc taccccacgc gtacaaccgc gtggggagac gacgcggatt
    51961 tttaactatg cgtatccccc gcatttatca tcctgaacca ctgaccagcc attctcacat
    52021 cgcgctttgc gaagatgccg ccaaccatat cgggcgcgta ctgcgcatgg ggccggggca
    52081 ggcgttgcaa ttgtttgacg gtagcaacca ggtctttgac gccgaaatta ccagcgccag
    52141 caaaaaaagc gtggaagtga aggtgctgga aggcaagatc gacgatcgcg aatctccgct
    52201 gcatattcac ctcggtcagg tgatgtcgcg tggtgaaaaa atggaattta cgatccagaa
    52261 atcgatcgaa ctcggtgtaa gcctcattac gccacttttt tctgagcgct gcggcgttaa
    52321 actggatagt gaacgtctga acaagaagct ccagcagtgg cagaagattg caattgctgc
    52381 ctgtgagcag tgtggtcgta accgggtgcc ggaaatccgc ccagcgatgg atctggaagc
    52441 ctggtgtgca gagcaggatg aaggactgaa actgaatctt cacccgcgcg ccagtaacag
    52501 catcaatacg ttgccgttac cggttgaacg cgtccgcctg ctgattggcc cggaaggcgg
    52561 tttatcggca gatgaaattg ccatgactgc ccgctatcaa tttactgata tcctgttggg
    52621 acctcgcgtt ttgcgtacag agacaactgc gctcaccgcc attaccgcgc tacaagtacg
    52681 atttggcgat ttgggctaac ggagaagaat aatgatcaag ctcggcatcg tgatggaccc
    52741 catcgcaaac atcaacatca agaaagattc cagtttcgct atgttgctgg aagcacagcg
    52801 tcgtggttac gaacttcact atatggagat gggcgatctg tatctgatca atggcgaagc
    52861 ccgcgcccat acccgcacgc tgaacgtgaa gcagaactac gaagagtggt tttcgttcgt
    52921 cggtgaacag gatctgccgc tggccgatct cgatgtgatc ctgatgcgta aagacccgcc
    52981 gtttgatacc gagtttatct acgcgaccta tattctggaa cgtgccgaag agaaagggac
    53041 gctgatcgtt aacaagccgc agagcctgcg cgactgtaac gagaaactgt ttaccgcctg
    53101 gttctctgac ttaacgccag aaacgctggt tacgcgcaat aaagcgcagc taaaagcgtt
    53161 ctgggagaaa cacagcgaca tcattcttaa gccgctggac ggtatgggcg gcgcgtcgat
    53221 tttccgcgtg aaagaaggcg atccaaacct cggcgtgatt gccgaaaccc tgactgagca
    53281 tggcactcgc tactgcatgg cgcaaaatta cctgccagcc attaaagatg gcgacaaacg
    53341 cgtgctggtg gtggatggcg agccggtacc gtactgcctg gcgcgtattc cgcagggggg
    53401 cgaaacccgt ggcaatctgg ctgccggtgg tcgcggtgaa cctcgtccgc tgacggaaag
    53461 tgactggaaa atcgcccgtc agatcgggcc gacgttgaaa gaaaaagggc tgatttttgt
    53521 tggtctggat atcatcggcg accgtctgac tgaaattaac gtcaccagcc caacctgtat
    53581 tcgtgagatt gaagcagagt ttccggtgtc gatcaccgga atgttaatgg atgccatcga
    53641 agcacgttta cagcagcagt aacccacctt agcgagaagg atctcgttga gactctgagt
    53701 gacagcgccc ttctttccac gcatactggg cgctgttgct tttttgaacc aggaaacaga
    53761 acctctgaca atgaatttac agcatcactt tcttattgcc atgcctgctc tccaggatcc
    53821 gattttccgt cgttccgtgg tctacatttg cgaacataat accaatggtg caatggggat
    53881 catcgtcaac aagccgctgg aaaatctcaa aattgaaggg attctggaaa agctgaagat
    53941 cacgccggag ccgcgtgatg aatcaatccg tctggataaa ccggttatgc tcggcggtcc
    54001 gctggctgaa gatcgcgggt ttattttgca tactccgccc tccaattttg cttccagcat
    54061 tcgcatttca gacaacacag tgatgaccac ttcccgcgat gtgctggaaa cgctcggcac
    54121 cgataaacaa ccgtctgacg tattggtggc tctgggttat gcctcctggg aaaaaggtca
    54181 actggaacaa gaaattctcg ataacgcgtg gctaacggcc ccggcggatc tgaatattct
    54241 gttcaaaacg ccgattgccg accgctggcg cgaggcggca aaactgattg gtgtggatat
    54301 tctcaccatg cctggtgtgg cagggcacgc ctgatgagtg gaaccttact cgccttcgac
    54361 ttcggcacca aaagcattgg cgtagcggtc ggccaacgca ttaccggcac tgctcgccct
    54421 ttgcctgcaa ttaaagcaca ggacggtacg ccggactgga acctcatcga acgtttactg
    54481 aaagagtggc agccggacga aatcatcgtc ggtttgccgc tgaatatgga cggcaccgag
    54541 caaccattga ctgccagagc gcgtaaattt gccaaccgta ttcatggtcg tttcggtgtt
    54601 gaagtaaagc tccatgacga acgtcttagc accgtggaag cccgttccgg tctgtttgaa
    54661 cagggcggct atcgggcgct caacaaaggc aaagtcgact ctgcctctgc ggttattatt
    54721 ctcgaaagct atttcgagca gggatattaa ggcgatctaa aagcctggcg gatagtcgtt
    54781 accgccatct tgccggatgc ggcgtgaacg ccttatccag cacacatctg gcagcggctg
    54841 taggtctgat aagacgcgtt agcgtcgcat cagacattta ttgcctttat aggccctata
    54901 aacttgcgca tcgggcatgg caacgtcaca aacgcccttc ccccacccgc tgctgataac
    54961 tctgctgaaa cgttaacatc cccacctgct gcccggtttg aataacatgc ggtaactggt
    55021 gggttttccc ttcgcgaatc aaattcccca ccgcgggagt gttaatcaat aattcaaaca
    55081 gcgccacgcg tccttcctgt ttatccactt ccagcttttg tgacaacacg gcccgtaaac
    55141 tacctgccag ttgattacgt acgggatctt tttcctgcgc cggaaatgaa tccaccagtc
    55201 gctcaactgc ctgcgcggca ccacgcgtat gtaatgttgc cagcaccaaa tgcccggttt
    55261 ctgccgccgt cagtgccaga cggattgtct cgctgtcacg cagctctccg agcaaaatca
    55321 catcaggatc ttcccgcaat gcggcccgca atcccgatgc gaacgtcata cagtgcaaac
    55381 caatttcccg ctgctggatc aaacatcgct ggctggcata gatatattca acaggatctt
    55441 ccagcgtcag aatatgcgca tcggcatgtt gatagagata gccaaccatc gccgctagcg
    55501 tggtagattt gccgctcccc gtcgcccccg tcaccagaat caggccattc tcgctcttga
    55561 gtaattccgg caataccgtt ggtgcgccaa gctgttcgag ctgtggacag tgcgaaggta
    55621 acaaccgtaa tgccagagaa ataccttgcc gttgcgcgaa cgcactgcca cgcaaccgct
    55681 ggttttccgc cagcgacacg gcaaaatcca gctggccatt ttccagcaat attgcccgct
    55741 gatcgtcatc cagccactcc cgcagtagct cttcgacgtc cggcgcatca aacggcgcag
    55801 cttccattag cccgcgaatg cgccatcgtg cggaccaggc gctgcacagg tgtagatccg
    55861 agacgttatg ctttacacta agggccacaa tttcttccat attcatacta agatcctcgg
    55921 aaaatgaacg atattgcgca taacctggca caggtccggg acaaaatctc agcggctgca
    55981 acgcgttgcg gccgttctcc agaagaaatt acgctgcttg cagtcagtaa aacaaaacct
    56041 gcgagcgcca tcgcagaagc cattgatgcc gggcagcgtc aatttggtga aaactacgtt
    56101 caggaagggg tagataaaat tcgccacttt caggaactgg gcgtaacagg attagaatgg
    56161 cattttattg gcccgttgca gtctaataaa agccgcctgg tggcagagca tttcgactgg
    56221 tgtcatacca tcgaccgttt gcgcatcgct acccgcctca acgatcagcg cccggcagaa
    56281 cttccccctc ttaacgtact gattcaaatt aatattagtg atgaaaacag taagtccggg
    56341 attcaactgg cagaactgga cgagctggca gctgcggtcg ctgaactacc gcgtttacgt
    56401 ctgcgcgggc tgatggcaat ccctgcacct gagtcagaat atgtaaggca gtttgaagtt
    56461 gcacgccaaa tggctgtagc atttgccgga ctgaaaacgc gctacccgca tatcgacacg
    56521 ctctctctgg gaatgtcgga cgatatggaa gccgccattg cggcaggtag cacgatggtt
    56581 cgtatcggca ctgcaatttt tggtgcgcgt gattactcta aaaaataagg aattaaagga
    56641 acgccatgaa tacgttgact ttcctgcttt caacggtcat tgagctgtat accatggtgc
    56701 tgttattacg catctggatg cagtgggctc attgtgattt ttacaacccc ttctcacagt
    56761 ttgtagtgaa ggtaacgcag ccaattatcg ggccactgcg ccgcgttatt ccggcaatgg
    56821 gccccattga cagcgcctcg ctgctggttg cctatattct cagttttatc aaagccatcg
    56881 tgctgtttaa agtggtgacc ttcctgccaa tcatctggat tgccggttta ctgattctgc
    56941 tgaaaaccat cggcctgctg attttctggg tcctgctggt gatggcgatt atgagctggg
    57001 taagccaggg tcgtagcccg attgaatacg tgctgattca gctggccgat ccgctgctgc
    57061 gcccgattcg ccgcctgcta ccggcaatgg gtgggattga tttctcgccg atgatcctcg
    57121 ttctgctgct gtatgtcatc aatatgggtg tcgcagaagt attacaggca accggaaaca
    57181 tgctgctgcc ggggctgtgg atggcgttat gagtgccgta acagttaatg atgacggtct
    57241 ggttttacgg ctctatattc agccgaaagc cagccgtgat tctattgtcg gtttacatgg
    57301 cgacgaagtt aaagtcgcca ttaccgcgcc gccggttgac ggtcaggcca acagtcatct
    57361 ggtgaagttt ctcggtaagc aattccgggt tgccaaaagc caggtggtga ttgaaaaagg
    57421 cgaacttggc cgccacaaac aaattaaaat cattaatccg caacaaatcc cgccagaaat
    57481 cgcggcgtta attaattagg tatcctatgc aaaaagttgt cctcgcaacc ggcaatgccg
    57541 gtaaagtgcg tgagctggcg tcgctgctta gcgacttcgg tcttgatatc gtggcccaaa
    57601 cagacctcgg cgtagattcc gctgaagaaa ccggcctgac ctttatcgaa aacgcgattc
    57661 tgaaagcgcg ccatgcggca aaagtgaccg gtttaccggc aattgccgac gactctggtc
    57721 tggcggtaga tgtgcttggc ggcgcgccgg gcatttactc cgcgcgttat tccggtgaag
    57781 acgcgaccga tctaaagaat ctgcaaaaac tgctggaaac actgaaagac gtaccggatg
    57841 accaacgtca ggcgcgtttc cactgcgtgc tggtatatct gcgtcacgcg gaagatccga
    57901 ctccgctggt atgccacggt agctggccgg gcgtgattac tcgtgaacca gcgggcactg
    57961 gtggctttgg ttatgatcca atcttcttcg taccttccga agggaaaacc gccgccgaac
    58021 tgacccacga agaaaagagc gccatttccc accgtggtca ggcattgaaa ctgctgctgg
    58081 acgctttacg taatggttaa attaccgccg ctgagtctct acattcacat cccgtggtgc
    58141 gtgcagaaat gcccgtactg cgatttcaac tctcacgcgt tgaaaggaga agtgccgcac
    58201 gacgattacg ttcagcatct gctttgcgat ctggacaacg atgtggctta cgctcagggc
    58261 cgtgaagtaa agacaatctt tattggcggt ggtacgccga gcctgctttc cggcccggcg
    58321 atgcaaacgc tgctggacgg cgtgcgtgcg cgtttgccgc tggcagcgga tgcagaaatt
    58381 actatggaag cgaaccctgg tacggtagaa gccgatcgct ttgtcgatta tcagcgtgct
    58441 ggtgtgaacc gcatctctat tggtgtgcag agttttagcg aagacaagct gaaacgactt
    58501 gggcgtattc atggcccgca agaagcgaaa cgcgcggcaa agctggcgag cgggctgggg
    58561 ctgcgtagct ttaacctcga tttgatgcat ggactaccgg atcaatcact ggaagaggcg
    58621 cttggcgatc tacgccaggc cattgaactg aatccgccgc atctttcctg gtatcaactg
    58681 accatcgaac ctaatacgct gtttggttca cgcccgccgg tactgccgga cgacgacgcg
    58741 ctatgggata tattcgaaca ggggcatcag ttattaaccg cagcgggtta tcagcaatat
    58801 gaaacttccg cttacgccaa atccagttat cagtgccagc acaatctcaa ctactggcgc
    58861 tttggcgact acatcggtat tggctgcggc gcgcacggca aagtgacctt cccggatggg
    58921 cgcattctgc gtaccactaa aacgcgtcat ccgcgtggtt ttatgcaagg aaggtatctg
    58981 gaaagccagc gtgatgtcga agccgcagat aagccgtttg agttctttat gaatcgcttc
    59041 cgtctgctgg aggccgcgcc gcgcgtggag tttattgcgt ataccgggct ttgcgaagat
    59101 gtgattcgcc cacagttaga cgaggcgatt gcccagggtt atctcaccga atgtgcggat
    59161 tactggcaga taacggaaca tggtaaatta ttcttaaact cacttcttga attattcctc
    59221 gctgagtaat cattaat
//
LOCUS       DAZGOB010000013        58525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGOB010000013 DAZGOB010000000
VERSION     DAZGOB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.013"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..441)
                     /gene="folK"
                     /locus_tag="ACHLIF_000851"
     CDS             complement(<1..441)
                     /gene="folK"
                     /locus_tag="ACHLIF_000851"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO9604911.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREV"
     gene            complement(438..1835)
                     /gene="pcnB"
                     /locus_tag="ACHLIF_000852"
     CDS             complement(438..1835)
                     /gene="pcnB"
                     /locus_tag="ACHLIF_000852"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO9604912.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1895..2791)
                     /gene="gluQRS"
                     /locus_tag="ACHLIF_000853"
     CDS             complement(1895..2791)
                     /gene="gluQRS"
                     /locus_tag="ACHLIF_000853"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO9604913.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2858..3313)
                     /gene="dksA"
                     /locus_tag="ACHLIF_000854"
     CDS             complement(2858..3313)
                     /gene="dksA"
                     /locus_tag="ACHLIF_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO9604914.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3491..4195)
                     /gene="sfsA"
                     /locus_tag="ACHLIF_000855"
     CDS             complement(3491..4195)
                     /gene="sfsA"
                     /locus_tag="ACHLIF_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO9604915.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4210..4740)
                     /gene="thpR"
                     /locus_tag="ACHLIF_000856"
     CDS             complement(4210..4740)
                     /gene="thpR"
                     /locus_tag="ACHLIF_000856"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO9604916.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4814..7243
                     /gene="hrpB"
                     /locus_tag="ACHLIF_000857"
     CDS             4814..7243
                     /gene="hrpB"
                     /locus_tag="ACHLIF_000857"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO9604917.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRQEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7375..9909
                     /gene="mrcB"
                     /locus_tag="ACHLIF_000858"
     CDS             7375..9909
                     /gene="mrcB"
                     /locus_tag="ACHLIF_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO9604918.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10129..12372
                     /gene="fhuA"
                     /locus_tag="ACHLIF_000859"
     CDS             10129..12372
                     /gene="fhuA"
                     /locus_tag="ACHLIF_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO9604919.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLSGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12423..13220
                     /gene="fhuC"
                     /locus_tag="ACHLIF_000860"
     CDS             12423..13220
                     /gene="fhuC"
                     /locus_tag="ACHLIF_000860"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO9604920.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13220..14110
                     /gene="fhuD"
                     /locus_tag="ACHLIF_000861"
     CDS             13220..14110
                     /gene="fhuD"
                     /locus_tag="ACHLIF_000861"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO9604921.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14107..16089
                     /gene="fhuB"
                     /locus_tag="ACHLIF_000862"
     CDS             14107..16089
                     /gene="fhuB"
                     /locus_tag="ACHLIF_000862"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO9604922.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVHAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16124..17404)
                     /gene="hemL"
                     /locus_tag="ACHLIF_000863"
     CDS             complement(16124..17404)
                     /gene="hemL"
                     /locus_tag="ACHLIF_000863"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO9604923.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17570..17617
                     /gene="yadX"
                     /locus_tag="ACHLIF_000864"
     CDS             17570..17617
                     /gene="yadX"
                     /locus_tag="ACHLIF_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO9604924.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17629..19050
                     /gene="clcA"
                     /locus_tag="ACHLIF_000865"
     CDS             17629..19050
                     /gene="clcA"
                     /locus_tag="ACHLIF_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO9604925.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDGARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19074..19139
                     /gene="yadW"
                     /locus_tag="ACHLIF_000866"
     CDS             19074..19139
                     /gene="yadW"
                     /locus_tag="ACHLIF_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO9604926.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19132..19476
                     /gene="erpA"
                     /locus_tag="ACHLIF_000867"
     CDS             19132..19476
                     /gene="erpA"
                     /locus_tag="ACHLIF_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO9604927.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19523..20146)
                     /gene="yadS"
                     /locus_tag="ACHLIF_000868"
     CDS             complement(19523..20146)
                     /gene="yadS"
                     /locus_tag="ACHLIF_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO9604928.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20184..20984)
                     /gene="btuF"
                     /locus_tag="ACHLIF_000869"
     CDS             complement(20184..20984)
                     /gene="btuF"
                     /locus_tag="ACHLIF_000869"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO9604929.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20977..21675)
                     /gene="mtnN"
                     /locus_tag="ACHLIF_000870"
     CDS             complement(20977..21675)
                     /gene="mtnN"
                     /locus_tag="ACHLIF_000870"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO9604930.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21759..23276
                     /gene="dgt"
                     /locus_tag="ACHLIF_000871"
     CDS             21759..23276
                     /gene="dgt"
                     /locus_tag="ACHLIF_000871"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO9604931.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23406..24830
                     /gene="degP"
                     /locus_tag="ACHLIF_000872"
     CDS             23406..24830
                     /gene="degP"
                     /locus_tag="ACHLIF_000872"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO9604932.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLGDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            24987..26138
                     /gene="cdaR"
                     /locus_tag="ACHLIF_000873"
                     /pseudo
     CDS             24987..26138
                     /gene="cdaR"
                     /locus_tag="ACHLIF_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26227..26613)
                     /gene="yaeH"
                     /locus_tag="ACHLIF_000874"
     CDS             complement(26227..26613)
                     /gene="yaeH"
                     /locus_tag="ACHLIF_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO9604933.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26775..27587)
                     /gene="yaeI"
                     /locus_tag="ACHLIF_000875"
     CDS             complement(26775..27587)
                     /gene="yaeI"
                     /locus_tag="ACHLIF_000875"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO9604934.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27642..28466)
                     /gene="dapD"
                     /locus_tag="ACHLIF_000876"
     CDS             complement(27642..28466)
                     /gene="dapD"
                     /locus_tag="ACHLIF_000876"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO9604935.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28497..31169)
                     /gene="glnD"
                     /locus_tag="ACHLIF_000877"
     CDS             complement(28497..31169)
                     /gene="glnD"
                     /locus_tag="ACHLIF_000877"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO9604936.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31231..32025)
                     /gene="map"
                     /locus_tag="ACHLIF_000878"
     CDS             complement(31231..32025)
                     /gene="map"
                     /locus_tag="ACHLIF_000878"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO9604937.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHTVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32393..33118
                     /gene="rpsB"
                     /locus_tag="ACHLIF_000879"
     CDS             32393..33118
                     /gene="rpsB"
                     /locus_tag="ACHLIF_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO9604938.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33376..34227
                     /gene="tsf"
                     /locus_tag="ACHLIF_000880"
     CDS             33376..34227
                     /gene="tsf"
                     /locus_tag="ACHLIF_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO9604939.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34374..35099
                     /gene="pyrH"
                     /locus_tag="ACHLIF_000881"
     CDS             34374..35099
                     /gene="pyrH"
                     /locus_tag="ACHLIF_000881"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO9604940.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35391..35948
                     /gene="frr"
                     /locus_tag="ACHLIF_000882"
     CDS             35391..35948
                     /gene="frr"
                     /locus_tag="ACHLIF_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO9604941.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36040..37236
                     /gene="ispC"
                     /locus_tag="ACHLIF_000883"
     CDS             36040..37236
                     /gene="ispC"
                     /locus_tag="ACHLIF_000883"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO9604942.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37425..38183
                     /gene="ispU"
                     /locus_tag="ACHLIF_000884"
                     /gene_synonym="uppS"
     CDS             37425..38183
                     /gene="ispU"
                     /locus_tag="ACHLIF_000884"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO9604943.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNSGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38196..39053
                     /gene="cdsA"
                     /locus_tag="ACHLIF_000885"
     CDS             38196..39053
                     /gene="cdsA"
                     /locus_tag="ACHLIF_000885"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO9604944.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39065..40417
                     /gene="rseP"
                     /locus_tag="ACHLIF_000886"
     CDS             39065..40417
                     /gene="rseP"
                     /locus_tag="ACHLIF_000886"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO9604945.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40447..42879
                     /gene="bamA"
                     /locus_tag="ACHLIF_000887"
     CDS             40447..42879
                     /gene="bamA"
                     /locus_tag="ACHLIF_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO9604946.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43001..43486
                     /gene="skp"
                     /locus_tag="ACHLIF_000888"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43001..43486
                     /gene="skp"
                     /locus_tag="ACHLIF_000888"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO9604947.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43490..44515
                     /gene="lpxD"
                     /locus_tag="ACHLIF_000889"
     CDS             43490..44515
                     /gene="lpxD"
                     /locus_tag="ACHLIF_000889"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO9604948.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44620..45075
                     /gene="fabZ"
                     /locus_tag="ACHLIF_000890"
     CDS             44620..45075
                     /gene="fabZ"
                     /locus_tag="ACHLIF_000890"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO9604949.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45079..45867
                     /gene="lpxA"
                     /locus_tag="ACHLIF_000891"
     CDS             45079..45867
                     /gene="lpxA"
                     /locus_tag="ACHLIF_000891"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO9604950.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45867..47015
                     /gene="lpxB"
                     /locus_tag="ACHLIF_000892"
     CDS             45867..47015
                     /gene="lpxB"
                     /locus_tag="ACHLIF_000892"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO9604951.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47012..47608
                     /gene="rnhB"
                     /locus_tag="ACHLIF_000893"
     CDS             47012..47608
                     /gene="rnhB"
                     /locus_tag="ACHLIF_000893"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO9604952.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47645..51127
                     /gene="dnaE"
                     /locus_tag="ACHLIF_000894"
     CDS             47645..51127
                     /gene="dnaE"
                     /locus_tag="ACHLIF_000894"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO9604953.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNRALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51140..52099
                     /gene="accA"
                     /locus_tag="ACHLIF_000895"
     CDS             51140..52099
                     /gene="accA"
                     /locus_tag="ACHLIF_000895"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO9604954.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52198..54339
                     /gene="ldcC"
                     /locus_tag="ACHLIF_000896"
     CDS             52198..54339
                     /gene="ldcC"
                     /locus_tag="ACHLIF_000896"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO9604955.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVALGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54396..54785
                     /gene="yaeR"
                     /locus_tag="ACHLIF_000897"
     CDS             54396..54785
                     /gene="yaeR"
                     /locus_tag="ACHLIF_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9604956.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54850..56148
                     /gene="tilS"
                     /locus_tag="ACHLIF_000898"
     CDS             54850..56148
                     /gene="tilS"
                     /locus_tag="ACHLIF_000898"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO9604957.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56197..56457)
                     /gene="rof"
                     /locus_tag="ACHLIF_000899"
     CDS             complement(56197..56457)
                     /gene="rof"
                     /locus_tag="ACHLIF_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO9604958.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLILTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56444..56644)
                     /gene="yaeP"
                     /locus_tag="ACHLIF_000900"
     CDS             complement(56444..56644)
                     /gene="yaeP"
                     /locus_tag="ACHLIF_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO9604959.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56810..57355
                     /gene="yaeQ"
                     /locus_tag="ACHLIF_000901"
     CDS             56810..57355
                     /gene="yaeQ"
                     /locus_tag="ACHLIF_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO9604960.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57352..57774
                     /gene="arfB"
                     /locus_tag="ACHLIF_000902"
     CDS             57352..57774
                     /gene="arfB"
                     /locus_tag="ACHLIF_000902"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO9604961.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57788..58498
                     /gene="nlpE"
                     /locus_tag="ACHLIF_000903"
                     /gene_synonym="cutF"
     CDS             57788..58498
                     /gene="nlpE"
                     /locus_tag="ACHLIF_000903"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO9604962.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13735 a        14539 c        16304 g        13947 t
ORIGIN      
        1 gacttcacgc aacgtctccc catcaggaaa cgccagctcc ggtgcaatct caaacagcgg
       61 ccacagcata aatccacgat tcttcatatc gtagtgcgga acggtcaggc gttcggtatt
      121 tatcacttca ttaccaaaca gcatgatatc gagatccagc gtgcgtggtc cccagcgttc
      181 agctttacga acacgaccct gttgcagttc tatgcgctgt gtatggttga gtaactcttc
      241 cggggcgagc gaagtttcaa gcgccacggc ggcgttgagg taatcgggtt gatcttgcgg
      301 ccccagcggt ggggtgcggt aaaacgaaga aacggcaaga atgtggcttt cagggatatc
      361 gcctaatgct ttcagggcag cattgacctg ctccagcgga gaggccagat tgctgcctat
      421 ggcaatatac gccactgtca tgcggtaccc tcacgacgcg gtgcgcgttt gcgtggacga
      481 cgagtacggc gacgcggtga cggctcttca tccaactcgt taagcatccc tttttgatct
      541 ggtggcgcgg aaacctggaa ctcaccccac catttcacca gacgctgcag ttcagcgtta
      601 cgttcaactt cagctcgcaa ggccaacagg tcataagccg cacggaactt aggatgctcc
      661 agcagtttcc atgcgcgttt accctgacga cgggacatac gcaactgcaa ctgccagata
      721 tcgcgggtta acgtcgtcag acgtttcggg attgccagtg aacggcaggc ttcgtccagc
      781 acgtcgttca tcgccagcgc gaaagcgtcg tgataggtca ggccgctttc ctgggcaatc
      841 ttctgtgccg tctccagcag tgggtaccag aacatggcgg caaacaggaa cgccgggttc
      901 acgcgcatat cgttatggat acgcgtatcg gtattcttca gcacctgttc aatgatccgc
      961 tccatcgggc tgtcgccatt ttccgtgaag tagcgggtaa tggtcgggaa cagcggctgg
     1021 aacagatgat attcacacaa cagcttatag gtatcgtaac cgtagcccgc ttgtagcagt
     1081 ttaagcgatt cttcaaacag gcgtgccggt gggatatcgt tcaacagggt agcgaggcga
     1141 gggatcggtt ctgcggtttc cgggctgatg cgcataccca atttggcggc aaaacgtacc
     1201 gcgcgcagca tacgtaccgg atcttcacgg tagcgcgttt ccgggttacc aatcagacgg
     1261 ataacgccgt ccttcagatc cttcataccg ccaacgtaat cacggacggt aaaatccgct
     1321 acgctgtaat agaggctgtt gatagtgaaa tcgcggcgct gggcgtcttc ttcgatggag
     1381 ccgaaaatgt tgtcgcgcag caacatgccg ttttgcccgc gttgggaggt cgtgcggtcg
     1441 ctgacgttac cttcgtggtg tccacggaag gtcgcaactt cgataatctc cgggccaaac
     1501 attacatgag ccagacggaa acggcgaccc accaggcggc agttacggaa cagtttgcgc
     1561 acctgctcag gcgtggcgtt agtggttacg tcaaaatctt tcggcttttt gccaagtaac
     1621 aggtcgcgca cgccgccgcc aaccagccag gcttcgtatc ccgctttgtt gagcctgtac
     1681 attaccttca gggcattttc actgatatct ttgcgggaaa tagcatgctg ctcacgcggg
     1741 atcaccgtca cctgtggacg ggcgactgcc tgttcagcct cgctttcctc gcggcttagc
     1801 accttgcggc aaaaattagc gactcgggta aaaatagtac acctcggtag tgtcaaacat
     1861 cattcaggac aaaaaaatag cggctaatca tagctcagca tgacgcattt gagaatgttg
     1921 aatttacaat tgccgactcg ggcacggcgg taagccgcca gtttttgacg gctgactgaa
     1981 ggatttgctc gacgctgaaa tcctgccagt gtgcttctgc ctgctgcccc agaaattgaa
     2041 gtgccgcgat tagtaccggg cgtggatcgc ctttcggcaa cgcaggcgca tgattctgct
     2101 tggaaagttt agcgccttgt ggattaagcg ccagcggcag atgaatgtaa tctggcactt
     2161 tccagccaaa aagctggtac agcgagattt gccttactgt tggttcaatc agatcagccc
     2221 cacgcactat ttctgtaacg ccctggaaat gatcatcaac cacaacagcc aggttgtagg
     2281 cgaacaaccc atcacggcga tgaatgataa aatcttcccg tgccagtttt tcgtcggcgt
     2341 gaataatgcc gcgcagctgg tcagtaaatt gcgtgaccgg atgctgctgg cggatacgca
     2401 ctgcggcatt gtctggtcca tgatgcaaca cccggcaatg accgtcgtaa ataccgccaa
     2461 tgctttgaat acgcgcacgc gtacaggtgc agtaataact tagtccttgt tcatgtaacc
     2521 aggcgagtgc ttcacgatag gcgtggtgac gttgcgattg ccagagaaca tcgccgtccc
     2581 agtgcagacc gtaatgttcc agctggcgca ggatagtttc tgcggcaccg ggaacttcac
     2641 gaggcgggtc gatatcttct atgcgtacca gccagcgacc ttgccgggcg cgagcctgca
     2701 aatagctgcc gagcgcggcg atcagagagc caaaatgaag ctcgccggaa ggagaggggg
     2761 cgaagcggcc aatatactgt gtgtctgtca tctctttgaa caaaaaataa ggcgggagca
     2821 tttcccgcct gtggtaaacg tgatggaacg gctgtaatta gccagccatc tgtttttcgc
     2881 gaatttcagc cagcgttttg cagtcgatgc acagatcggc tgtcgggcgc gcttccagac
     2941 ggcgaatacc aatttcaaca ccgcaggatt cgcagtagcc gaaatcttcg tcttccactt
     3001 ttttcagcgt cttctcgatc tttttgatca gcttacgctc gcgatcgcgg ttacgcagtt
     3061 cgaggctgaa ctcttcttcc tgggctgcac ggtctaccgg gtccgggaag ttggctgctt
     3121 catcctgcat atgtgtaacg gtgcgatcga cttcatccct gagttgatta cgccatgctt
     3181 ccagaatacg acggaagtgc gccagctggg cttcattcat atactcttcg cccggcttct
     3241 cctgatatgg ttccacccca gcgatggcga gaatactcag ggacgatgtt ttacggtttt
     3301 gcccttcttg catgttgctt ctccttaaca cgcactatcg atccccatgt tcgggggaaa
     3361 aatgaggccg ctataaatag cagatgcttt tccggatagc aattatctaa acgtaacact
     3421 tgacaactgt gtgaggaaaa gcgtatttgc gcacgcgacc agaatgtaaa ttaaccagtt
     3481 acttacttta ctacaatgta accggcagtg attttttaag agccatgcct tcagcagaaa
     3541 tttccgcttt gtaagccaga atttctaccc ccctctgttg agcttctgac aatagttgcg
     3601 cgtatttctc atcgatgtgg cgcgcgggtg aaaaccgtgt aatggctgaa tgcagcacgg
     3661 cgaaaaagat aaccgcacgc tggccttcag ccgctacgct catcaactcc cgaaggtgtt
     3721 tctgacctcg ttcagtgacc gcatcgggaa aatatccctg ttcgttctcc gctaacgtaa
     3781 ccgatttcac ttcaatatag cagtctggac gcgaatccgc ctgcaacata aagtcaatac
     3841 ggctgcgttc tgcgccgtat tttacttcgc ttttcagcga gctatagcct gacagttctg
     3901 aaattgattc attaaggata gcctctttcg tcaacctgtt agcccaaagc gtgttgacgc
     3961 aaataaatgc gccgctctgg ctttgagtta attcccaggt gtgtgggtat ttccgtttgg
     4021 tgttgtctga agtcgaatac cagacggtat cgccaggcgt tgcacaaccg gtcattgcac
     4081 ccgtattcgg gcagtgtagc gttaattcgc gaccatcggg tgtgatcaca tcggctaaaa
     4141 aacgtttgta acgctgaatt agcgtcgcgc gctgtagagg gggagaaaat tccattgcga
     4201 caatccttgt tattgcgtta gcgcccagcg ttttagcggc gtgtagcgtg tgcgtccacg
     4261 ggcaaacgag gaggcgtaaa gggtgaactc cgtcaccgca tacgaccagt taaaacctgg
     4321 cggcgggatt gtcaccgcct cgctggcgtc gcgcaataag gtaatatgtg gatgaaacgg
     4381 acgattgctt tgaaaacaac cgctgcgggc agcctgtgaa cggagcatat tcgccagctg
     4441 gattaagccg cgtggcggct gacgcatccc taaccacacc acacgcgaac gcagccattg
     4501 tccggcgtca tcaagcgtga gtgtgaaacc aggttgacga atccgtccgg ctaaaagaga
     4561 aagcgccttc tctttctcgg cgctcacttc gcctaaaaat gccagagtca gatgcaaatt
     4621 atcggcggcg accggacgtc ccgcctcagg tgggaagtgt gtggcgcgcc agtggataat
     4681 ctgttcgcgg atttctgcag gtaagtcgat agcaaagaac agacgttgcg gttcagacat
     4741 gtgaggcact cggttatgaa ttaccgcgat gctacaatgt ggcgcgaaga atgttaaccc
     4801 tctggagcgt tttgtgtcgt cgttgcccgt tgctgccgtc ttacctgaat tacttaccgc
     4861 tctcgattgt gcgccgcagg tattattaag tgcgccgacc ggggccggga aatcaacctg
     4921 gctgccgctg caactgctgg cgcatcccgg cattaacggg aaaattatcc tgctggagcc
     4981 gcgtcgtctg gcggcgcgta acgtcgcgca acggctggcg gagctgctta acgaaaagcc
     5041 aggcgatacc gttggctacc ggatgcgtgc gcaaaactgc gtcgggccga atacccgcca
     5101 ggaagtagtt accgaaggcg tgctgacgcg gatgatccag cgtgacccgg agttgagcgg
     5161 tgttggattg gtgatcctcg atgagtttca tgagcgcagc ttgcaggcgg atttggcgtt
     5221 ggcgctgtta ctcgatgtgc aacaaggtct gcgtgatgac cttaaactgc tgattatgtc
     5281 ggctacgctg gacaacgacc gcttgcagca aatgctgcca gaagcgcccg tcgtcatctc
     5341 agaagggcgc tcgtttccgg ttgaacgccg ttatttacca ctgcccacgc atcagcgttt
     5401 tgacgaagcc gttgcggtag ccaccgctga aatgctgcgt caggaaagtg gatcattact
     5461 gttattttta cctggcgtcg gagaaattca gcgtgtgcag gaacaactgg cttcgcgcat
     5521 cggcagcgat gtattgctct gcccgctgta tggcgcgttg tcgctgaacg atcagcgaaa
     5581 agcgatcctc ccggcaccgc aagggatgcg caaagtggtg ctggcgacca atattgctga
     5641 aaccagttta accatcgaag gtattcgtct ggtggtggat tgtgcccagg agcgtgtggc
     5701 gcgttttgat ccgcgcacgg ggcttacgcg actgattact caacgcgtta gccaggcatc
     5761 catgacgcag cgtgccgggc gcgccgggcg tctggagccg ggtatctgcc tgcatttaat
     5821 cgccaaggaa caagcagaac gcgccgcggc gcaaagtgaa ccggagatat tacaaagcga
     5881 tctttccggt ttgctgatgg aattactgca atggggatgc agcgatccgg cgcagatgag
     5941 ctggctggat caaccgccag tagtgaatct actggccgcg aaacgtctgt tacaaatgct
     6001 gggggcactg gagggtgaac ggcttagtgc gcaagggcaa aaaatggcag cgctgggtaa
     6061 cgatccgcgt ttagcggcaa tgctggttag cgcgaagaac gacgacgaag ctgctaccgc
     6121 ggcaaaaatt gccgccattc tcgaagagcc gccacggatg ggcaatagtg acctgggcgt
     6181 ggcgttttcg cgcaatcaac cagcctggca gcaacgtagt cagcaactgt taaaacgctt
     6241 aaacgtacgt ggcggtgagg cagacagttc gcttatcgcg ccgctacttg ccggggcgtt
     6301 tgccgatcgc attgctcgtc gccgtgggca agatggacgc tatcaactgg caaacggcat
     6361 gggagcgatg ctcgatgcca acgacgcgct aagccgccac gaatggttga tcgcaccgtt
     6421 attattgcag ggcagcgcct cgccggatgc gcggatttta ctggcgctgc cggtcgatat
     6481 tgatgagtta gtacaacgct gcccgcagct ggtacagcag tctgacactg tggagtggga
     6541 tgacgcgcaa ggtacgctga aagcctggcg tcggctgcaa atcggtctgc tgacggtgaa
     6601 agtgcagccg ctggcgaaac cctcggaaga cgagttgcat caggcgatgc ttaatggcat
     6661 ccgcgataaa gatttaagcg tgctcaactg gacggcggaa gcggaacagc tacgcttgcg
     6721 tttgttatgc gccgcaaagt ggttgccgga atatgactgg ccagcggttg atgatgaaag
     6781 tttattggcg acgctggaaa cgtggctgct gccacatatg actggcgtac attcactacg
     6841 cggcctgaaa tcactcgaca tttatcaggc actacgcgga ttacttgatt ggggaatgca
     6901 gcaacgtctg gatagtgaat tgcctgcgca ttacactgtg ccgacgggaa gccggatcgc
     6961 cattcgttat catgaagata acccgcccgc gctggcggtg agaatgcaag agatgtttgg
     7021 cgaggccacc aatccgacga tcgcccaggg gcgcgtgccg ctggtgctgg agttgctttc
     7081 acctgcccaa aggccattac aaatcacacg agatttgagc gccttctgga aaggagcgta
     7141 ccgtgaggtg caaaaagaga tgaaagggcg ttatcccaaa catgtctggc cggacgaccc
     7201 ggcaaatact gcaccgacgc gacggacgaa aaagtattcg taaggttggt tttctccctc
     7261 tccctgtggg agaggggacc gatcgagcac aaattttgag agatatcttc ttctgtcttg
     7321 taacagaaga acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc
     7381 gggaatgacc gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag
     7441 gtaagccgtc gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat
     7501 gaagaaccga tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa
     7561 cgcggctggc tatggctgct gttaaaactg gctatcgttt ttgccgtgct gatcgccatt
     7621 tacggcgttt atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg
     7681 cctgcggcag tttatggccg aatggtcaac cttgagccag atatgaccat cagcaagaac
     7741 gagatggtga agctgctgga ggcgactcag tatcgtcagg tgtcgaaaat gacccgtcct
     7801 ggcgaattta ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg
     7861 gacagtaaag aagggcaggt gcgtgcgcgt ctgacctttg atggcgatca tctggcgacg
     7921 atcgtcaata tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc
     7981 accatgatct cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct
     8041 gatttgctgg tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgacggc
     8101 atcagcctct actccatcgg acgtgcggtg ctggcaaacc tgaccgccgg acgcacggtg
     8161 cagggagcga gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagtgagcgt
     8221 tcttactggc gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc
     8281 aaagaccgta ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac
     8341 gaaatccgcg gcttcccgct ggcgagcttg tattactttg gtcgcccggt agaagagcta
     8401 agcctcgacc agcaggcgct gttagtcggc atggtgaaag gagcgtccat ctacaacccg
     8461 tggcgtaacc caaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag
     8521 caacagatta ttgatcaaga actctatgac atgttgagtg cacgtccgct gggtgttcag
     8581 ccacgcggtg gggtgatctc tccacagcca gcctttatgc aactggtgcg tcaggagctg
     8641 caggcgaaat taggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt
     8701 gactcggtgg cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag
     8761 aaacagcgta agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt
     8821 gaagttcgtg cgatggtcgg tggttctgag ccgcagtttg cgggctacaa ccgtgcgatg
     8881 caggcgcgtc gttcgattgg ttcccttgca aaaccagcga cttatctgac tgccttaagc
     8941 cagccgaaaa tctatcgtct gaatacgtgg attgcggatg cgccaatcgc gctgcgtcag
     9001 ccgaatggcc aggtctggtc accgcagaat gatgaccgtc gttatagcga aagcggcaga
     9061 gtgatgctgg tggatgcgtt gacccgttcg atgaacgtgc cgacggtaaa tctggggatg
     9121 gcgctggggc tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag
     9181 ttgcatccgg ttccggcgat gctgctgggg gcgttgaact taacgccaat cgaagtggcg
     9241 caggcgttcc agaccatcgc cagcggcggt aaccgtgcac cgctttctgc actgcgttcg
     9301 gtaattgcgg aagatggcaa ggtgctgtat cagagcttcc cgcaggcgga acgcgctgtt
     9361 ccggcgcagg cggcgtatct gacactatgg accatgcagc aggtggtaca acgcggtacg
     9421 ggtcgtcagc ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc
     9481 aacaataacg tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg
     9541 gtaggccgtg ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt
     9601 tatcagcgtt atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat
     9661 attgcagata tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc
     9721 ttgccggtct ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag
     9781 ccgtcaggca atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct
     9841 gctcagcaag agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt
     9901 agtaattaac atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat
     9961 cccatctaag atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc
    10021 tcagattgcg taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat
    10081 aatcattctc gtttacgtta tcattcactt tacatcagag atataccaat ggcgcgttcc
    10141 aaaactgctc agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggta
    10201 agcggcatgt ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt
    10261 accgctgcac ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcga
    10321 cagtctgcta ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct
    10381 gttgtgaccg ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc
    10441 tacacgccgg gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc
    10501 attcgcggct ttgcggcaga aggccaaagc cagaataact atctgaatgg cctgaagttg
    10561 cagggcaact tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt
    10621 atgcgtggcc cggtttccgt gctttacggt aaaagcagtc ctggcggcct gttgaatatg
    10681 gtcagcaagc gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac
    10741 agcctgttcc agactggttt tgactttagc gattcgttgg atgatgacgg tgtttactct
    10801 tatcgcctga ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag
    10861 cgttatgcta ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc
    10921 ctttcttact tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga
    10981 accgttgagc cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag
    11041 aacaacacct attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac
    11101 gacaccttta ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc
    11161 gtttatggtt acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca
    11221 ttagcgccag cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag
    11281 ctgcaaaact tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac
    11341 cacaccctgc tgtccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt
    11401 ggttacgacg actctgtgcc actgctcaat ctgtacaatc cggtgaatac cgatttcgac
    11461 ttcaatgcca aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa
    11521 acgggcgttt atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt
    11581 cgttatgact gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt
    11641 gatgacaaac agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca
    11701 ccttacttca gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat
    11761 attttcgcac cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat
    11821 cgtccgattg tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg
    11881 gaccctgagg gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa
    11941 atcgaagcga aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc
    12001 gatgcggaat acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa
    12061 cacatggctt cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg
    12121 ctgggcaccg gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa
    12181 gtgggaagtt atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg
    12241 gcgggctcca acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc
    12301 tgctttaaca cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc
    12361 ttccgtttct aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg
    12421 ttatgcagga atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc
    12481 gtgtgcccgg gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga
    12541 ccggtctgat tggtcacaac ggttctggta aatccactct gctcaaaatg cttggccgtc
    12601 atcagcctcc gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca
    12661 gcaaagcgtt tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga
    12721 tgaccgtgcg tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct
    12781 ttggtgcggc agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc
    12841 tggcgcatcg gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca
    12901 tgctggtggc gcaggatagc cgttgtctgt tgctcgacga accgacttcg gcgctggata
    12961 tcgcccacca ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga
    13021 cggtcattgc cgtgctgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg
    13081 ccctgcgcgg tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa
    13141 ccctcgaaat gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac
    13201 ctgtgagttt tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac
    13261 ggcgatggcg ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga
    13321 ccccaatcgt attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat
    13381 cgtgccttac ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt
    13441 gccggactca gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga
    13501 aatgaaaccg tcgtttatgg tctggtcggc aggatatggc ccttcaccag aaatgctggc
    13561 acgtattgcg ccgggtcgcg gatttaactt cagtgacggc aaacagccgt tggcgatggc
    13621 gcgtaaatcg ctgacggaaa tggcagattt acttaacctg caaagcgcag cagaaacgca
    13681 tttagcgcac tatgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc
    13741 acgcccgtta ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa
    13801 cagcttgttc caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac
    13861 caacttctgg ggcagtaccg ccgtcagtat cgatcgtctg gcggcgtata aagacgttga
    13921 tgtgctctgt tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct
    13981 gtggcaggcc atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt
    14041 ttatggtgca acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg
    14101 taaagcgtga gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt
    14161 gtcgctacgg cgctaacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg
    14221 caggccgcct ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg
    14281 ttgccgcgtc tggcgatttc gctgctggtg ggcgcaggcc tggggctggt gggcgtgctg
    14341 tttcagcaag tgctgcgtaa tccgctggcg gaaccgacga cacttggcgt tgctacaggc
    14401 gcgcaactgg gaattaccgt caccacactt tgggcgatcc caggtgctat ggcgagccag
    14461 tttgccgcac tggtgggcgc ttgtgtcgtt ggcttaatcg tctttggcgt cgcgtggggg
    14521 aaacggcttt cgccggttac gcttattctc gcggggctgg tggtgagcct ttattgcggg
    14581 gcaattaatc agctactggt tatcttccat cacgaccagc tgcaaagcat gttcctgtgg
    14641 agcaccggaa cgctgacgca aaccgactgg ggcggcgttg agcgtttatg gccgcagttg
    14701 ctgggcggcg tgatgttgac gttattgcta cttcgcccgt taaccctgat ggggcttgat
    14761 gatggcgtgg cgcgcaatct cgggctggcc ttgtcgctcg cgcgtctggc agcgctgtcg
    14821 ctggcgattg tcatcagtgc gctgctggtg cacgctgtgg ggattatcgg ctttatcggg
    14881 ttgttcgcgc cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg
    14941 ctggcgtcgc tgattggggc gctgatcctc tggctttccg atcaaatcat cctctggctg
    15001 actcgcgtgt ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg
    15061 ctactgtggc tgttgccgcg tttacgcagc attagcgcgc cggatatgaa ggtcaacgat
    15121 cgtgtcgcgg ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgctg
    15181 ttgatggctg tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg
    15241 agcggggcgt tgcttgagga tttgatgccc tggcgctggc cgcgaattat ggcggcgctg
    15301 tttgcgggcg tcatgctggc ggtggcgggc tgtattattc agcggctgac cggaaacccg
    15361 atggcaagcc cggaagtgct ggggattagc tctggcgcag cgtttggcgt ggtgttgatg
    15421 ctgtttctgg tgccgggtaa tgcctttggc tggctgttac ctgcaggcag tctcggcgcg
    15481 gcggtgacgc tgttgatcat tatgatcgcc gccggccgcg gtggattttc cccacaccgc
    15541 atgttactgg cggggatggc gttaagcacc gcgttcacca tgcttttgat gatgttgcag
    15601 gcaagtggtg acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac
    15661 gcgaccgatg cgcaggtctg gcgcacggga attgtgatgg tgattttgct ggcgattacc
    15721 ccgctgtgcc gccgctggct gaccatttta ccgctgggtg gtgataccgc ccgagccgta
    15781 ggaatggcgc tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg
    15841 accgcgacga tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg
    15901 cggatgatgg gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt
    15961 ttactgctgg tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatccca
    16021 gcagggctgc tgtcgacctt tatcggcgcg ccatatttta tctatttgtt gagaaagcag
    16081 agccgttaat agaatgcctc gcccggtggg cgaggcaaaa tcatcacaac ttcgcaaaca
    16141 cccgtcgtgc agcatcgatg gtgttattga tatcttccat gctgtgcgcc acggacataa
    16201 agcccgcttc aaacgctgac ggtgccaggt aaacaccttc gtccagcatc atatggaaga
    16261 aacgcttaaa gcgttccacg tcacaggcca tcacatcctg atagcacgtc acggactcgg
    16321 cgtcggtaaa gaaaataccg aacatgccgc caacgtggtt aacgaccagc ggaattccgg
    16381 cttcttctgc cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt
    16441 cgtgaacgcc cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaattg
    16501 ggttaccgga aagcgtaccc gcctgataga ccggacccgt cggggccagc gcatccatta
    16561 catcacgacg accaccgaat gcgcctaccg gcattccacc gccgatgatt ttgcccaggc
    16621 aggtgagatc cggttccaca ccgtaataat cctgtgcgcc agctagcgct acgcggaagc
    16681 cagtcattac ttcatcgatg atcagcaatg cgccaaattc gtcgcacagc gcgcgcagac
    16741 ctggcaggaa ctctggcagc ggtggaacgc agttcatatt gcctgccacc ggctcgacga
    16801 taatacaggc aatctcttgc gggtattgct caaacgcggc gcgtacagaa gccagatcgt
    16861 tataagtaca ggttaaggta tgtttggcga aatctgccgg aacacccggc gagtttggct
    16921 ggcctaacgt gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt
    16981 aacaaccttc aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg
    17041 cgctcatcgt cgcttcagtg ccggagttca ccatgcgcac catatccatg gtcgggacca
    17101 gttcggtcac cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac
    17161 cacgctcggc ggcttcaatc acggcattgc ggatagccgg atggttatgg cccagcacca
    17221 tcggccccca ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag
    17281 cgccgtccgc tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag
    17341 gggagttcac accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac
    17401 tcatggaggg ttcctgattc gtagaaaaag tgaatggctg ctattctatg ttattcataa
    17461 caagttaaat actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga
    17521 gtataaaagt tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc
    17581 gagtaaaatg ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat
    17641 actccctctt tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa
    17701 cttcttgagc gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg
    17761 cttgtcgggc tggcagcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt
    17821 atgggggcgc tggtacatac tgctgataat tatccgcttc tgttaaccgt cgcttttctc
    17881 tgttcggcgg tgctggcgat gttcggctac tttttggtgc gcaaatacgc gccggaagca
    17941 ggtggttcgg ggatcccgga aattgaaggg gcgctggaag atcaacgtcc cgttcgctgg
    18001 tggcgtgtgt tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatggtg
    18061 ctagggcgcg aaggaccaac cgtgcagatc ggcggcaata tcggtcgcat ggtgcttgat
    18121 attttccgcc tgaaaggtga tggagctcgc catacgctgc tggcaaccgg tgctgctgcg
    18181 gggctggctg cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg
    18241 cgtccgcagt ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg
    18301 tcgaccatta tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt
    18361 tctgatgcgc cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt
    18421 ttcggcccta tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac
    18481 ggcggcaata ttaccaaatg ggtactgatg ggtggtgcga ttggcggtct gtgtggattg
    18541 ctggggtttg tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc
    18601 gcggggaatt tcagcatggg aatgctggtg tttattttcg tcgctcgggt cattaccacc
    18661 ttactctgct tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt
    18721 actgtgctgg gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg
    18781 gaggcgggga cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg
    18841 ccgttaacgg ggatcattct ggttctggag atgaccgata actaccagct cattttgcca
    18901 atgattatta ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaaccgcta
    18961 tactcggcga ttcttgcgcg cacgctggca aaacaggaag ctatgcaact ggcgcgaagc
    19021 aaggccgcat cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga
    19081 ttattattgg gttagaattt gcccaattgc cgatgtcgtt tggagcaaaa tatgagtgat
    19141 gacgtagcac tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc
    19201 gctgacgaag ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc
    19261 ggcttccagt atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa
    19321 aaacagggcg ttggcttggt ggttgatccg atgagcctgc aatatctggt cggcggttcc
    19381 gttgactata ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa
    19441 agcacctgcg gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa
    19501 ctatccggca catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct
    19561 taagatgcca acgaatagcc gccagccgaa tcaagagcgt cacgaccatg cccatcatac
    19621 tggctgtttc cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga
    19681 taatacaggc agttgcgtag atttctgtac gtaaaatcat ggggatttcg cgggccagaa
    19741 catcacgaat gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg
    19801 gaccggcttc cgcattaaag gctttattca cgccaatgcc gacaaacacc gccagaccaa
    19861 cggcgtccag caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga
    19921 tggtcagcat gctggttacc attgcaacga ccagatcggt gggatctttc acccaaaata
    19981 ccgggccgtg atccagcgcc atgtcgcgaa ttgtcccgcc gcctacagcg gtaaccacgc
    20041 ccagtaccag aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccggaga
    20101 tggcaaatac cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac
    20161 tgagcacctg aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt
    20221 gcagcgagga taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg
    20281 ggaattttga gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct
    20341 gtaatgacaa tcgcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag
    20401 ggaacccggc tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga
    20461 atcgactctt ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt
    20521 ttaggtttat cagcatattg cgctttcaat tgcgcgtact gatccagcag ggattgcgcg
    20581 gcttgttcgg ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca
    20641 atttgttcaa tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg
    20701 tcaacctgcc gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg
    20761 acaatgcgtt ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt
    20821 ggaggatagt cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt
    20881 tcagtgttgg cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgca
    20941 agaaaagaca gggcgaccag cgccctgaac agtgacttag ccatgtgcaa gtttctgcac
    21001 cagtgactca accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct
    21061 aagatgagac tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt
    21121 gaaattgtgg cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg
    21181 gaagttgtgg cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac
    21241 aatcaggcca cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat
    21301 cagtttatcg tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc
    21361 aaatgccgtg acatccgcgt cgtgataacg tgcttcgtcc gagacaacga tatcgcccac
    21421 tttcaacgtt ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca
    21481 gtgttccaac agcaaagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag
    21541 aagcgcaacc tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat
    21601 agtttgacgt ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat
    21661 gatgccgatt ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg
    21721 cgcttacggg gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa
    21781 aaaaaataaa ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga
    21841 tcctgcggat cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc
    21901 tgcaacaaaa gacgcaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta
    21961 cccactcgat ggaagtccag caggtggggc gctacatcgc caaagaaatt ttaagccgtc
    22021 tgaaagagct taaattgctg gaagcatacg gcctggatga actgaccgga ccttttgaaa
    22081 gcattgttga gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg
    22141 gcgaagcagc gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc
    22201 agcctctgac tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac
    22261 cgcttaacga gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac
    22321 aaggcattcg tctggtgcat acattgatgc ggatgaatct cacctgggca caggttggcg
    22381 gtattttaaa atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact
    22441 atttaatgaa aaagccgggt tattatcttt ctgaagaagc ctatattgcc cggttgcgta
    22501 aagaacttaa tttggcgctt tacagtcgtt ttccattaac ttggattatg gaagctgccg
    22561 acgacatctc ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg
    22621 ttgagcagct ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct
    22681 tttcgctggt ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta
    22741 cggaagatca gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg
    22801 cggcacaacg atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat
    22861 tattggaaga tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa
    22921 aacatgtgtt tagccatcca gatgtcgagc agcttgaatt gcagggctat cgggtcatta
    22981 gcggattatt agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac
    23041 tggtagaaaa agaacgggtg aaacgtttcc ctattgaatc gcgcttattc cacaaactct
    23101 cgacgcgcca tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg
    23161 agtttccgct atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta
    23221 tgaccgacct ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag
    23281 gcttttgtaa agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc
    23341 gcgctataaa acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga
    23401 aatacatgaa aaaaaccaca ttagcactga gtgcactggc tctgagttta ggtttggcgt
    23461 tatctccgct ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc
    23521 caagccttgc accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag
    23581 gtagcacaac cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg
    23641 attctccgtt ctgccaggaa ggttcaccgt tccagagctc tccgttctgc caggggggcc
    23701 agggcggtaa tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca
    23761 ttgatgccga taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga
    23821 ttaaagtcca gctgggcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc
    23881 gctctgatat cgcgctgatt cagatccaga acccgaaaaa cctgaccgca attaagatgg
    23941 cggattctga tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggcc
    24001 tgggagagac ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgccg
    24061 aaaactacga aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg
    24121 cgctggttaa cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg
    24181 gcggcaacat cggtatcggt tttgctatcc cgagcaacat ggtgaaaaac ctgacctcgc
    24241 agatggtgga atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga
    24301 attccgaact ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg
    24361 ttctgcctaa ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac
    24421 tgaacggtaa gccaatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg
    24481 tgggcagcaa actgaccctg ggcttgctgc gcgacggtaa gcaggtcaac gtgaatctgg
    24541 aactgcagca gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag
    24601 gcgctgagat gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaaaacgg
    24661 gcactccggc tgcgcagatc ggcctgaaga aaggtgatgt gattattggc gcgaaccagc
    24721 aggcagtgaa aaacatcgct gaactgcgta aagttcttga cagcaaaccg tctgtactgg
    24781 cactgaacat tcagcgcggc gacagctcca tctacctgtt aatgcagtaa tctccctcaa
    24841 ccccttcctg aaaacgggaa ggggttctcc ttacaatctg tgaacttcac cacaactcca
    24901 tacatcttca tcatccttta ggcatttgca caatgccgta cgttacgtac ttccttatgc
    24961 taagccgtgc ataacggagg acttatggct ggcatcttga taccaaaatg gcgcaggata
    25021 tcgtggcacg taccatgcgc atcatcgata ccaatatcaa cgtaatggat gcccgtgggc
    25081 gaattatcgg cagcggcgat cgtgagcgta ttggtgaatt gcacgaaggt gcattgctgg
    25141 tactttcaca gggacgagtc gtcgatatcg atgacgcggt ggcacgtcat ctgcacggtg
    25201 tgcggcaagg gattaatcta ccgttacggc tggaaggtga aattgtcggc gtaattggcc
    25261 tgacaggtga accagagaat ctgcgtaaat atggcgaact ggtctgcatg acggctgaaa
    25321 tgatgctgga acagtcgcgg ttgatgcact tgttggctca ggatagccgt ttgcgggagg
    25381 aactggtgat gaacctgatt caggcagagg agaatactcc cgcacttact gaatgggcgc
    25441 aacggctggg gatcgatctc aatcaaccgc gagtggtggc tattgttgag gtcgacagcg
    25501 gtcagcttgg tgtggacagc gcaatggcgg agttacaaca actgcaaaac gcgctgacta
    25561 cgcccgagcg taataatctg gtggcgattg tctcgctaac cgaaatggtg gtgttgaaac
    25621 cggcgttgaa ctcttttggg cgctgggatg cagaagatca tcgtaagcga gttgaacaac
    25681 tgattacccg catgaaagag tacggccagc tgcgttttcg cgtttcactg ggcaactatt
    25741 ttaccggtcc tggcagtatt gcccgatcct atcgtacggc gaaaacgacg atggtggtgg
    25801 gtaaacagcg gatgccagaa agtcgctgct atttttatca ggatctgatg ttacctgtgt
    25861 tactcgacag tttgcgtggc gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc
    25921 tgaaagcgat ggacaataac ggcttgctgc gacgaacgct ggcggcgtgg tttcgtcaca
    25981 atgtgcaacc gctggcaacg tcaaaggcgt tgtttattca tcgtaatacc ctggagtatc
    26041 ggcttaatcg tatatcggaa ctgaccgggc ttgatttggg caattttgat gacaggttgc
    26101 tgctgtatgt ggcgttacaa ctggatgaag agcggtaggt tatgcgttaa ggttgaggcg
    26161 gcgctggctc attcccctca gcccgaaggg gagaggggaa tgcccgaatt aaccgtcaat
    26221 tcgggattat ttacgcgtta gtttttccag atcggcttca atctcgctga tcttattggt
    26281 tacgactgac tccaggtgac gtaagtcgtc gaggatctta cgcttaagat caacttcgct
    26341 gcggtcacgc tggcagattt gatcaagctc atcaatgata taccgtagat tcgggctgat
    26401 ttcctggact tctttataac cctgacccac accatcagcg acgaccgttt tacgctgacg
    26461 cggatattta aacttaacgc tcttggcgaa aaactcgcct ttgtctttct ggaaatagat
    26521 tttcagaata tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt cttcaggatt
    26581 ggtaataccc agacttttca gattgtcgta catagcgtta cctcaaaatg agtcagtaaa
    26641 ttgtgcttat tttagcattt ggcctggccc gcccggctcg actgtgatcg cagaaagcct
    26701 ttttcttcca gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt tattgtggtg
    26761 ataaaattca cgttctacac cagttccagc atcgttactt ccgggcggca gttcagacgc
    26821 aaaccataca aactgcccac gccacgggtt gtgtagatgt gtcgttcgcc aaaggcattt
    26881 aatccggcga cgtaacgttt atcttcgaca ggggcaaaag gttcaccaac caacggtacg
    26941 cgcagttgcc cgccgtgagt atggccgcac agcatcagat cccagggttc atcacgcatg
    27001 acttctttgc tgtcgggatt atgcgccagc accagtcttg gcagattggc ttcgctggcg
    27061 ggaggcggtt tgcattgtcc ggcccataag tcaccggtgc ctaccagttc gaattgcctg
    27121 ttcggcgtgg cgatcaccgt agcctggtta aacaacaccg tgatgcccgc tgatttcaac
    27181 gtctcgccaa ttaagtgatt tttttctgta ccaacagggc gatcgtggtt gccgaagcag
    27241 gcaaacgtcg gcgcacattc ggcaaggggg gagaggacat cactaaacgc cgaaaaattc
    27301 agcggcatat caaataatac gtaatcgccg cccagtaata tcaaatcggg cttttgttct
    27361 atgccaagag caatcgcgtc agaaatcagg cttaaaggaa caaaacgaga gtaatggaga
    27421 tcggccagaa aaagaatttt gaatggtgct gcgttgtctt taaaaaaggc gaggcggtga
    27481 cggattaatt caaaccagcc tggctcccag taatgcatat aaccgaaacc tgagctcgtg
    27541 gctatcgtcg cggcagtagc ctgcaacaat cggcggcgtg aaatcatcgc tcatccctgc
    27601 aaaaaaaatc gggcagcgtc gtgctgcccg tgtgcatact tttagtcgat ggtacgcagc
    27661 agttcgttaa tgccgacttt gccgcgagtt ttcgcgtcaa ctttcttaac gataaccgca
    27721 cagtagaggc tgtatttgcc atcttttgac ggcagattac ctgaaacaac cacagacccc
    27781 gccggaacgc gaccgtagtg gatttcgccg gtttcacggt cgtaaatacg ggtgctctga
    27841 ccaatgtata cgcccatgga aatgacggaa ccttcttcga caatcacccc ttcaaccact
    27901 tcagagcgtg cgccaatgaa gcagttatct tcaatgatgg ttgggttagc ctgcagcggt
    27961 tccagcacgc cgccgatgcc cacgccaccg gaaaggtgga cgtttttacc aatctgcgca
    28021 caagaaccga cggtcgccca ggtatcaacc atggtgcctt catcaacata tgcgccgatg
    28081 ttgacgtaag acggcatcag cacggtgtta cgggcaataa acgcaccctg acgtaccgcc
    28141 gctggtggca caacgcggaa gccttctttc tggaaacgtg cttcgtcgta gtcggcgaat
    28201 ttcatcggca ctttgtcgaa gtagcggctt tctgcccctt cgatcacctg attatcatta
    28261 atacggaaag agagcagcac cgcttttttc aaccactgat gcgtcaccca ctgaccgtca
    28321 attttttccg ctacacgcag tgcaccggaa tccagcaggg cgatcacctg atttaccgct
    28381 tcgcgggtaa cggtgtctgc gttggctggc gtgatctcgg cacggcgttc aaaagcggtt
    28441 tcaataatgt tctgtaactg ctgcattgtt aaactctttt catatcagta aacacatcac
    28501 cctttatcgt ttggattgag ggcctctgtc aaccgctgat gcacttcctg ctgcaactcg
    28561 ttattaagtg cacgccggtc agcggtggca attatgaata aatcttctac tcgctcgcca
    28621 atggttgtaa ttcgggcacc atgaagcgaa attcccagat cggcaaaaat tttcccgact
    28681 ctcgccagca gtcctggctg gtcgagcgcg ataagctcaa ggaacgattt gcggtcggta
    28741 tgggtcggca aaaacgttac ttcggtttca acagtaaaat ggcgtaattt ggcgggttgg
    28801 cgacggggct gcggtggctg ccagctactt tgcgtcagta cttgctccag accaaaccga
    28861 ataacctcat gacgatctgc ggacagcggg ctgccatcgg gttccagcac gataaaggta
    28921 tccatcgcca taccgtcgcg agtggtgaaa atttgtgcgt cgtgaacact taaattgcgg
    28981 cggtctaatt cggcacagac ggcggcaaac agataggggc ggtccgggct ccagataaaa
    29041 atctcggtgc ctccacgcgt agcctgcggg ctaagcaata ccagcggttt gcttaaatca
    29101 tgctgtaata aatggcgggc atgccaggcc agttgatttg ggctatggcg gacaaaatag
    29161 ttagcacgac agcgtgacca aatttggtgc agcgcctctt cgtcgatgtt atccatgcgc
    29221 agtagtgcca gtgcctggag ttggtgatga cgaacccgtt cgcgcatatc cggcgtgttt
    29281 tgcattccgc gtcgtagctg cttttcggtg gcaaaataga gttcgcgcaa cagactttgc
    29341 ttccagctat tccacagcgt ttcgttggtg gcgcaaatgt cagccaccgt caggcatacc
    29401 agataacgaa gtcggttttc tgtttgcact tcttcggcaa actgcttgat gacttccggg
    29461 tcctgaatat cgcggcgttg ggcggtcacc gacatcaaca ggtgctggcg aaccagccag
    29521 gcaaccagct gtgtttcgcg cgagttaagc ccgtggagtt cggcaaaatg cactacatcc
    29581 tgagcaccga gaatggagtg gtcgccgccg cgtcctttgg cgatatcgtg aaacagcgcg
    29641 gcgatgaaaa tcagctcagt tgacggcagg cgcggccaga cgtccacaca caacggatgg
    29701 cgctggcgcg tttcttcact ggcaaaactc tccagtttca gcatcacgcg gatagtatgt
    29761 tcatccaccg tgtaggcgtg gaacagatca aactgcatct gcccgacgat atgcgaccat
    29821 tgcggcatat acgcgccgag cacgctatgg cgatgcattg gcaatagccc gcgccgcacc
    29881 gctccggggt gacgcagaat gctcaaaaac agttttcgtg cttccggaat attacacagc
    29941 ggttgttgca gatggcgacg ggcatggcgt aactggcgca gcgtggtgga gtaaatgccg
    30001 gtgatcgcac tgttgcgcac catggtgtag aacatacgca agatggcttc cggctggcgc
    30061 ataaatagtg tttcatcacg caggtcgatt agcgtaccgc gtagctgaaa ctcatcgtcg
    30121 agtggacgtg gtttttcgtc ggcgggaagg gcgaggatgg cttcatcgaa cagttgcagc
    30181 agcatctggt tgagttcact gacgcggcgt gtaacgcgga agtaatcctt catcatccgc
    30241 tcgaccggtt cgttgccttc accactgtaa ttcagacgct gagcgacgct aagttggcga
    30301 tcgaataaca gacgattatc gtaacggctg acgaccagat gcagggcaaa gcgaatacgc
    30361 cacagtatat gcagacattc gtttaattcc gcccgctccg ctgaggttaa gaagccaaac
    30421 ccaaccattt catccagcga tgttgcgcca aaatgacggc gggccaccca ttgcagagtg
    30481 tggatatcgc gcaagccgcc agggctactt ttgatgtctg gttcaagatt gtagctggtg
    30541 ccgtggtaac gctgatggcg ctggttctgt tcttcaactt tcgccgcgta gaacttgtcg
    30601 gaaggccaga atccttcgct aaaaatatgt ttttgcagtt cgaggaagag cgcaacatcg
    30661 ccaattaata agcgggattc gattaaattg gtggcgacgg ttaaatccga taacccttcc
    30721 agcatgcact cttcaagcgt gcgcacacta tgaccgactt ccagctttac atcccagagc
    30781 agcgttaaca gctcgcccac tttttgcgcc tgatcgtccg ggagcttttt acggcttaaa
    30841 atcagtaaat cgatgtcaga aagtggatgc agctcgccac gaccgtagcc accgacggcg
    30901 accaatgcca ggtcggcaat ctggctgaat cccgcttcaa tccataatcg ttgcaggagc
    30961 tggtcgataa actcggtgcg cgcctcaatc aactgttctg cagagatccc attgtcaaag
    31021 gcatcaccca gccaacgctg gaaagtatcg atatgggctt ttatcccacc gacggttaat
    31081 tcatcacggg gccagacgca tggattttgc ggttgaccgg gcagggtggg gagagcggtg
    31141 tttgcgtact gttctggaag ggtattcatt gtgcgccacc cataagatta aattatcgca
    31201 ttaaaaaagc cggcattcgc cggcttcatc ttattcgtcg tgcgagatta tcgccgggat
    31261 ggtgtcatcc ttgcgtagcg tcagaatttc gcagccgtta tcagtcacca caatagtatg
    31321 ctcatattgt gcagacaagc tgcgatcttt ggtttttacc gtccagccat ctttcatggt
    31381 gcggatctct tttttacccg cgttgaccat tggctcgatg gtgaacgtca tcccaggttt
    31441 cagtacgacg ttggtttcac gggagtcata gtgcagcacc tgcggttctt catggaagcc
    31501 gcgaccaata ccgtgtccgc aatattcacg aacgacggag aagccttctg cttcgacaaa
    31561 tttctgaatc gccgcaccga tttcgcgcag attaatgcct ggtttgacca tgcgcagcgc
    31621 caggtacagg ctttcttgcg tgatgcggca cagacgttcg cccatgatgg tcggcttacc
    31681 gacgataaac attttcgagg tatcgccgtg gaaaccatct ttgattacgg tgacatcaat
    31741 gttaacgata tcgccatctt tcagcagctt cgcatcgtcc gggataccgt ggcacaccac
    31801 ttcattaata gagatgcaaa cggatttcgg atagccgtga tagccgaggc aggcagaaac
    31861 cgtgtgttgt tcattaacaa tgtaatcatt acagatgcga tccagctcgc cggtgctgac
    31921 gcccggttta acatacggtt cgatcatctc cagcacttcg gcagccagtc ggccagcgac
    31981 gcgcattttt tcgatatctt ctggggtctt gattgagata gccattaatt ctgtccatca
    32041 gcgtcggtga taccgacaat atatatgtaa gtgccgtcaa tggtatcaca cccgggcaaa
    32101 ttgagaatca ttctgaattt cgccaaacgt gccactgaac gttttctata atagaaaatt
    32161 caacgtctga tgctgtacac agcgccaaca attattggtg tccatgacgt atttgtggta
    32221 taaagcgcgc cggacttccg atccatttcg tatacacaga ctggacggaa gcgacaatct
    32281 cactttgtgt aacaacacac acgtatcggc acatattccg gggtgccctt tggggtcggt
    32341 aatatgggat acgtggaggc ataaccccaa cttttatata gaggttttaa tcatggcaac
    32401 tgtttccatg cgcgacatgc tcaaggctgg tgttcacttc ggtcaccaga cccgttactg
    32461 gaacccgaaa atgaagccgt tcatcttcgg tgcgcgtaac aaagttcaca tcatcaacct
    32521 tgagaaaact gtaccgatgt tcaacgaagc tctggctgaa ctgaacaaga ttgcttctcg
    32581 caaaggtaaa atccttttcg ttggtactaa acgcgctgca agcgaagcgg tgaaagacgc
    32641 tgctctgagc tgcgaccagt tcttcgtgaa ccatcgctgg ctgggcggta tgctgactaa
    32701 ctggaaaacc gttcgtcagt ccatcaaacg tctgaaagac ctggaaactc agtctcagga
    32761 cggtactttc gaaaagctga ccaagaaaga agcgctgatg cgcactcgtg agctggagaa
    32821 actggaaaac agcctgggcg gtatcaaaga catgggcggt ctgccggacg ctctgtttgt
    32881 aatcgatgct gaccacgaac acattgctat caaagaagca aacaacctgg gtattccggt
    32941 atttgctatc gttgatacca actctgatcc ggacggtgtt gacttcgtta tcccgggtaa
    33001 cgacgacgca atccgtgctg tgaccctgta cctgggcgct gttgctgcaa ccgtacgtga
    33061 aggccgttct caggatctgg cttcccaggc ggaagaaagc ttcgtagaag ctgagtaata
    33121 aggcttgata actcccccaa aatagttcga gttgcagaaa ggcggcaagc tcgagaattc
    33181 ccgggagctt acatcagtaa gtgaccggga tgagcgagcg aagacaacgc atctgcggcg
    33241 cgaaatatga agggggagag cccttataga ccaggtagta cacgtttggt tagggggcct
    33301 gcatatggcc ccctttttca cttttatatc tgtgcggttt aatgccgggc agatcacatc
    33361 tccgaggatt ttagaatggc tgaaattacc gcatccctgg taaaagagct gcgtgagcgt
    33421 actggcgcag gcatgatgga ttgcaaaaaa gcactgactg aagctaacgg cgacatcgag
    33481 ctggcaatcg aaaacatgcg taagtccggt gctattaaag cagcgaaaaa agcaggcaac
    33541 gttgctgctg acggcgtgat caaaaccaaa atcgacggca actacggcat cattctggaa
    33601 gttaactgcc agactgactt cgttgcaaaa gacgctggtt tccaggcgtt cgcagacaaa
    33661 gttctggacg cagctgttgc tggcaaaatc actgacgttg aagttctgaa agcacagttc
    33721 gaagaagaac gtgttgcgct ggtagcgaaa attggtgaga acatcaacat tcgccgcgtt
    33781 gctgcgctgg aaggcgacgt tctgggttct tatcagcacg gtgcgcgtat cggcgttctg
    33841 gttgctgcta aaggcgctga cgaagagctg gttaaacaca tcgctatgca cgttgctgca
    33901 agcaagccag aattcatcaa accagaagac gtatccgctg aagtggtaga aaaagaatac
    33961 caggtacagc tggatatcgc gatgcagtct ggtaagccga aagaaatcgc agagaaaatg
    34021 gttgaaggcc gcatgaagaa attcaccggc gaagtttctc tgaccggtca gccgttcgtt
    34081 atggaaccaa gcaaaactgt tggtcagctg ctgaaagagc ataacgctga agtgactggc
    34141 ttcatccgct tcgaagtggg tgaaggcatc gagaaagttg agactgactt tgcagcagaa
    34201 gttgctgcga tgtccaagca gtcttaatta tcaaaaagga gccgcctgag ggcggcttct
    34261 ttttgtgccc atcttgtaaa ttcagctaac ccttgtgggg ctgcgctgaa aagcgacgta
    34321 caatgtcgct agtattaatt catttcaatc gttgacagtc tcaggaaaga aacatggcta
    34381 ccaatgcaaa acccgtctat aaacgcattc tgcttaagtt gagtggcgaa gctctgcagg
    34441 gcactgaagg cttcggtatt gatgcaagca tactggatcg tatggctcag gaaatcaaag
    34501 aactggttga actgggtatt caggttggtg tggtgattgg tgggggtaac ctgttccgtg
    34561 gcgctggtct ggcgaaagcg ggtatgaacc gcgttgtggg cgaccacatg gggatgctgg
    34621 cgaccgtaat gaacggcctg gcaatgcgtg atgcactgca ccgcgcctat gtgaacgctc
    34681 gtctgatgtc cgctattcca ttgaatggcg tgtgcgacag ctacagctgg gcagaagcta
    34741 tcagcctgtt gcgcaacaac cgtgtggtga tcctctctgc cggtacaggt aacccgttct
    34801 ttaccaccga ctcagcagct tgcctgcgtg gtatcgaaat tgaagccgat gtggtgctga
    34861 aagcaaccaa agttgacggc gtgtttaccg ctgatccggc gaaagatcca accgcaacca
    34921 tgtacgagca actgacttac agcgaagtgc tggaaaaaga gctgaaagtc atggacctag
    34981 cggccttcac gctggctcgt gaccataaat taccgattcg tgttttcaat atgaacaaac
    35041 cgggtgcgct gcgccgtgtg gtaatgggtg aaaaagaagg gactttaatc acggaataat
    35101 tcccgtgatg gataaataag ggtaagattc cgcgtaagta tcgcgggggc gttagtctgg
    35161 ttataaggcg ttattgttgc aggcagtttg gtcacggcca gcgcgcagca accggagcgt
    35221 acaaaagtac gtgaggatgg cgagcactgc tcggggccaa aatggcaaat aaaatagcct
    35281 aataatccag acgattaccc gtaatatgtt taatcagggc tatacttagc acacttccac
    35341 tgtgtgtgac tgtctggtct gactgagaca agttttcaag gattcgtaac gtgattagcg
    35401 atatcagaaa agatgctgaa gtacgcatgg acaaatgcgt agaagcgttc aaaacccaaa
    35461 tcagcaaaat acgcacgggt cgtgcttctc ccagcctgct ggatggcatt gtcgtggaat
    35521 attacggcac gccgacgccg ctgcgtcagc tggcaagcgt aacggtagaa gattcccgta
    35581 cactgaaaat caacgtgttt gatcgttcaa tgtctccggc cgttgaaaaa gcgattatgg
    35641 cgtccgatct tggcctgaac ccgaactctg cgggtagcga catccgtgtt ccgctgccgc
    35701 cgctgacgga agaacgtcgt aaagatctga ccaaaatcgt tcgtggtgaa gcagaacaag
    35761 cgcgtgttgc agtacgtaac gtgcgtcgtg acgcgaacga caaagtgaaa gcactgttga
    35821 aagataaaga gatcagcgaa gacgacgatc gccgttctca ggacgatgta cagaaactga
    35881 ctgatgctgc aatcaagaaa attgaagcgg cgctggcaga caaagaagca gaactgatgc
    35941 agttctgatt tcttgaacga caaaaacgcc gctcagtaga tccttgcgga tcggctggcg
    36001 gcgttttgct ttttattctg tctcaactct ggatgtttca tgaagcaact caccattctg
    36061 ggctcgaccg gctcgattgg ttgcagcacg ctggacgtgg tgcgccataa tcccgaacac
    36121 ttccgcgtag ttgcgctggt ggcaggcaaa aatgtcactc gcatggtaga acagtgcctg
    36181 gaattctctc cccgctatgc cgtaatggac gatgaagcga gtgcgaaact tcttaaaacg
    36241 atgctacagc aacagggtag ccgcaccgaa gtcttaagtg ggcaacaagc cgcttgcgat
    36301 atggcagcgc ttgaggatgt tgatcaggtg atggcagcca ttgttggcgc tgctgggctg
    36361 ttacctacgc ttgctgcgat ccgcgcgggt aaaaccattt tgctggccaa taaagaatca
    36421 ctggttacct gcggacgtct gtttatggac gccgtaaagc agagcaaagc gcaattgtta
    36481 ccggtcgata gcgaacataa cgccattttt cagagtttac cgcaacctat ccagcataat
    36541 ctgggatacg ctgaccttga gcaaaatggc gtggtgtcca ttttacttac cgggtctggt
    36601 ggccctttcc gtgagacgcc attgcgcgat ttggcaacaa tgacgccgga tcaagcctgc
    36661 cgtcatccga actggtcgat ggggcgtaaa atttctgtcg attcggctac catgatgaac
    36721 aaaggtctgg aatacattga agcgcgttgg ctgtttaacg ccagcgccag ccagatggaa
    36781 gtgctgattc acccgcagtc agtgattcac tcaatggtgc gctatcagga cggcagtgtt
    36841 ctggcgcagc tgggggaacc ggatatgcgt acgccaattg cccacaccat ggcatggccg
    36901 aatcgcgtga actctggcgt gaagccgctc gatttttgca aactaagtgc gttgacattt
    36961 gccgcaccgg attatgatcg ttatccatgc ctgaaactgg cgatggaggc gttcgaacaa
    37021 ggtcaggcag cgacgacagc attgaatgcc gcaaacgaaa tcaccgttgc tgcttttctt
    37081 gcgcaacaaa tccgctttac ggatatcgct gcgttgaatt tatccgtact ggaaaaaatg
    37141 gatatgcgcg aaccacaatg tgtggacgat gtgttatctg ttgatgcgaa cgcgcgtgaa
    37201 gtcgccagaa aagaggtgat gcgtctcgca agctgaggat aatccggcta cagagagtcg
    37261 cgctatttgt tagcgtaggg cttcagtgat atagtctgcg ccatctgatc gtaagtagtt
    37321 ggctttataa ggtcaggtat gccgtggttt tacacggctt ttttttgtat aggcttcagt
    37381 attcctgagt accgtaaacc ctgtcaggga ataaaaaacg cgtgatgttg tctgctactc
    37441 aaccacttag cgaaaaattg ccagcgcatg gctgccgtca tgttgcgatc attatggacg
    37501 gcaatggtcg ctgggcaaaa aagcaaggga agattcgtgc ctttgggcat aaagccgggg
    37561 caaaatccgt ccgccgggct gtctcttttg cggccaacaa cggtattgag gcgttaacgc
    37621 tgtatgcctt tagtagtgaa aactggaacc gaccagcgca ggaagtcagt gcgttaatgg
    37681 aactgtttgt gtgggcgctc gatagcgaag taaaaagtct gcaccgacat aacgtgcgtc
    37741 tgcgtattat tggcgatacc agtcgcttta actcgcgttt gcaagaacgt attcgtaaat
    37801 ctgaagcgct aacagccggg aatagcggtc tgacgctgaa tattgcggcg aactacggtg
    37861 gacgttggga tatagtccag ggagtcaggc aactggctga aaaggtgcag caaggaaacc
    37921 tgcaaccaga tcagatagat gaagagatgc taaaccagca tgtctgtatg catgaactgg
    37981 cccctgtaga tttagtaatt aggactgggg gggagcatcg cattagtaac tttttgcttt
    38041 ggcaaattgc ctatgccgaa ctttacttta cagatgttct ctggcccgat ttcgatgaac
    38101 aagactttga aggggcgtta aatgcctttg ctaatcgaga gcgtcgtttc ggcggcaccg
    38161 agcccggtga tgaaacagcc tgatgggggt cgcttttgct gaagtatcgc ctgatatctg
    38221 cttttgtgtt aatacccgtc gtcatcgcgg cgttgtttct gttgccgccg gtggggttcg
    38281 ccatcgtaac gctggtggtc tgcatgctgg cagcgtggga atggggacag cttagcggtt
    38341 ttaccactcg ttcgcagcga gtatggttgg cggtgttatg cgggttattg ttggcgctga
    38401 tgctttttct gttgccggaa tatcaccgaa atattcatca accgctggtt gaaatctcac
    38461 tttgggcttc gttgggttgg tggattgtcg cgctattgct ggtgctgttt tacccaggtt
    38521 ccgcagcaat ctggcgtaac tctaaaacat tgcgccttat ttttggcgtg ctaaccattg
    38581 ttcccttctt ctggggcatg ctggcgttac gggcctggca ctacgacgag aatcattaca
    38641 gtggcgcaat atggctgctc tatgtcatga tcctggtatg gggcgctgac tctggcgcat
    38701 atatgtttgg caaattgttt ggtaaacata agctggcacc gaaggtttct ccgggtaaaa
    38761 cctggcaagg ctttataggt ggactagcga ccgcagcggt aatctcatgg ggttatggca
    38821 tgtgggcgaa tctcgacgtc gctcccgtca ccttactcat ttgctctatt gtcgcagcgt
    38881 tagcctcagt gctcggcgat ctgaccgaga gtatgtttaa gcgtgaagca ggaattaagg
    38941 acagcggtca tttaattcca ggacacggtg gtattttaga tcgtattgat agcctgacgg
    39001 ctgcggtacc ggtctttgct tgcttgttgt tactggtatt caggacgctt taacggaagg
    39061 taatatgctg agttttctct gggatttggc ttcgttcatc gttgcactgg gtgtacttat
    39121 caccgtgcat gaatttggtc atttctgggt tgcccggcgt tgtggtgttc gcgttgagcg
    39181 tttctcaata gggtttggta aggcgctctg gcggcgaact gataagctcg gcaccgaata
    39241 tgttatcgcc ctgatcccgc tgggcggtta tgtcaaaatg ctggatgagc gcgcagaacc
    39301 ggtcgttccg gaactccgcc accatgcctt caataataaa tctgtcggcc aacgagcggc
    39361 gattattgcc gcaggtccgg ttgcaaactt catttttgct atctttgcct actggctggt
    39421 ttttattatt ggtgtgcctg gcgtacgtcc ggtggttggt gaaatagcag ccaattcgat
    39481 agctgcggaa gcacaaattg caccaggtac ggaactaaaa gccgtagatg gtatcgaaac
    39541 gcctgattgg gatgccgtgc gtttgcagtt ggtcgataaa attggcgatg aaagcaccac
    39601 cattacagta gcgccatttg gcagcgacca acggcgggat gtaaagctcg atttacgtca
    39661 ctgggcgttt gagcctgata aagaagatcc ggtatcttcg ctggggattc gtcctcgtgg
    39721 gccgcaaatt gaacctgtac tggaaaatgt gcaaccaaac tcggcggcaa gcaaggcagg
    39781 tttgcaagca ggcgacagga tcgttaaagt cgatggtcag cccttaacgc agtgggtgac
    39841 ctttgtgatg cttgtccggg ataacccggg taaatcctta gcgttagaaa tcgaaaggca
    39901 gggaagtccc ttgtctttga cattaatccc ggagagtaaa ccgggtaatg gtaaagcgat
    39961 tggttttgtc ggtattgagc cgaaagtcat tcctttgcca gatgagtata aagttgtacg
    40021 ccagtatggg ccgttcaacg ccatcgtcga agccacggac aaaacgtggc agctgatgaa
    40081 gctgacggtc agtatgctgg gaaaattgat caccggtgat gtgaaactga acaacctcag
    40141 tgggccgatc tctatcgcca agggggctgg gatgacagcg gaactcgggg tagtttatta
    40201 cctgccgttt cttgcgctta ttagcgtgaa cttagggata attaacctgt ttccgttgcc
    40261 cgtacttgac ggggggcatc tgctgttcct tgcgatcgaa aagatcaagg gcggaccggt
    40321 atccgagcgg gttcaagact tttgttatcg cattggctcg attctgctgg tgctgttaat
    40381 ggggcttgca cttttcaatg atttctctcg gttatgagag ttagttagga agaacgcata
    40441 ataacgatgg cgatgaaaaa gttgctcata gcgtcgctgc tgtttagcag cgccaccgta
    40501 tacggtgctg aagggttcgt agtgaaagat attcatttcg aaggccttca gcgtgtcgcc
    40561 gttggtgcgg ccctcctcag tatgccggtg cgcacaggcg acacggttaa tgatgaagat
    40621 atcagtaata ccattcgcgc tctgtttgct accggcaact ttgaggatgt tcgcgtcctt
    40681 cgtgatggtg atacccttct ggttcaggta aaagaacgtc cgaccattgc cagcattact
    40741 ttctccggta acaaatcggt gaaagatgac atgctgaagc aaaacctcga ggcttctggt
    40801 gtgcgtgtgg gcgaatccct cgatcgcacc accattgccg atatcgagaa aggtctggaa
    40861 gacttctact acagcgtcgg taaatatagc gccagcgtaa aagctgtcgt gaccccgctg
    40921 ccgcgcaacc gtgttgacct aaaactggtg ttccaggaag gtgtgtcagc tgaaatccag
    40981 caaattaaca ttgttggtaa ccatgctttc accaccgacg aactgatctc tcatttccaa
    41041 ctgcgtgacg aagtgccgtg gtggaacgtg gtaggcgatc gtaaatacca gaaacagaaa
    41101 ctggcgggcg accttgaaac cctgcgcagc tactatctgg atcgcggtta tgcccgtttc
    41161 aacatcgact ctacccaggt cagtctgacg ccagataaaa aaggtattta cgtcacggtg
    41221 aacattaccg aaggcgatca gtacaagctt tctggcgttg aagtgagcgg caaccttgcc
    41281 gggcactccg ctgaaattga gcagctgact aagatcgagc cgggcgagct gtataacggc
    41341 accaaagtga ccaagatgga agatgacatc aaaaagcttc tcggtcgcta tggttatgcc
    41401 tatccgcgcg tacagtcgat gcctgaaatt aacgatgccg acaaaaccgt taaattacgc
    41461 gtgaacgttg atgcgggtaa ccgtttctac gtgcgtaaga tccgttttga aggtaacgat
    41521 acctcgaaag atgccgtcct gcgtcgcgaa atgcgtcaga tggaaggtgc atggctggga
    41581 agcgatctgg tcgatcaggg taaggagcgt ctgaatcgtc tgggcttctt tgaaactgtc
    41641 gataccgata cccaacgtgt tccgggtagc ccggaccagg tagatgtcgt ctacaaggta
    41701 aaagagcgca acaccggtag cttcaacttc gggattggtt acggtactga aagtggcgtg
    41761 agcttccagg ctggtgtgca gcaggataac tggttaggta caggttatgc tgttggtatc
    41821 aacgggacca aaaacgatta ccagacctat gctgaactgt cggtaaccaa cccgtacttc
    41881 actgtagatg gtgtaagcct cggtggtcgt ctcttctata atgacttcca ggcagatgac
    41941 gccgacctgt ccgactatac caacaagagt tatggtacag acgtgacgtt gggcttcccg
    42001 atcaacgaat ataactcgct gcgtgcaggt ctgggttatg tacataactc cctgtccaac
    42061 atgcagcctc aggttgcgat gtggcgttat ctgtactcta tgggtgaaca tccgagcacc
    42121 tctgatcagg ataatagctt caaaacggac gacttcacgt tcaactatgg ttggacctat
    42181 aacaagcttg accgtggtta cttcccgaca gatggttcac gtgtcaacct gaccggtaaa
    42241 gtgaccattc ctggatcgga taacgaatac tacaaagtga cgttagacac ggcgacttat
    42301 gtgccgatcg atgacgatca caaatgggtt gttctggggc gtacccgctg gggttatggt
    42361 gatggtttag gcggcaaaga gatgccgttc tacgagaact tctatgccgg tggttccagc
    42421 accgtgcgtg gcttccagtc caatacaatt ggtccgaaag cagtttactt cccgcatcag
    42481 gccagtaatt atgatccgga ctatgattac gaatgtgcga ctcaggacgg cgcgaaagac
    42541 ctgtgtaaat cggatgatgc tgtaggcggt aacgccatgg cggttgccag cctcgagttc
    42601 atcaccccga cgccgtttat tagcgataag tatgctaact cggttcgtac ttccttcttc
    42661 tgggatatgg gtaccgtttg ggatacaaac tgggattcca gccaatattc tggatatccg
    42721 gactatagtg atccaagcaa tatccgtatg tctgcgggta tcgcattaca atggatgtcc
    42781 ccattggggc cgttggtgtt ctcctacgcc cagccgttca aaaagtacga tggagacaag
    42841 gcagaacagt tccagtttaa catcggtaaa acctggtaag tgttctccac aaaggaatgt
    42901 agtggtagtg tagcgatgac tttaggcgat caatataaga tcgccgggcc acgcaaagaa
    42961 ctgcaccctc cggtgcaaat gggatggtaa ggagtttatt gtgaaaaagt ggttattagc
    43021 ggcaggtctc ggtttagcac tggcaacttc tgctcaggcg gctgacaaaa ttgcaatcgt
    43081 caacatgggc agcctgttcc agcaggtagc gcagaaaacc ggtgtttcta acacgctgga
    43141 aaatgagttc aaaggccgtg ccagcgaact gcagcgtatg gaaaccgatc tgcaggctaa
    43201 aatgaaaaag ctgcagtcca tgaaagcggg cagcgatcgc actaagctgg aaaaagacgt
    43261 gatggctcag cgccagactt ttgctcagaa agcgcaggct tttgagcagg atcgcgcacg
    43321 tcgttccaac gaagaacgcg gcaaactggt tactcgtatc cagactgctg tgaaatccgt
    43381 tgccaacagc caggatatcg atctggttgt tgatgcaaac gccgttgctt acaacagcag
    43441 cgatgtaaaa gacatcactg ccgacgtact gaaacaggtt aaataagtaa tgccttcaat
    43501 tcgactggct gatttagcgc agcagttgga tgcagaacta cacggtgatg gcgatatcgt
    43561 catcaccggc gttgcgtcca tgcaatctgc acaaacaggt cacattacgt tcatggttaa
    43621 cccaaaatac cgtgagcatt taggcttgtg ccaggcgtcc gcggttgtca tgacccagga
    43681 cgatcttcct ttcgcgaaaa gtgccgcgct ggtagtgaag aatccctacc tgacttacgc
    43741 gcgcatggcg caaattttag ataccacgcc gcagcccgcg cagaacattg cacccagtgc
    43801 ggtgatcgac gcgacggcga agctgggtaa caacgtatcg attggcgcta acgcggtgat
    43861 tgagtccggc gttgaactgg gcgataacgt gattatcggt gccggttgct tcgtaggtaa
    43921 aaacagcaaa atcggtgcag gttcgcgtct ctgggcgaac gtaaccattt accatgagat
    43981 ccagatcggt cagaattgcc tgatccagtc cggaacagtg gtaggcgcag acggctttgg
    44041 ttatgccaac gatcgtggta actgggtgaa gatcccacag attggtcgcg taattattgg
    44101 cgatcgcgtg gagatcggtg cctgcacaac catcgatcgc ggcgcgctgg atgacactat
    44161 tattggcaat ggcgtgatca ttgataacca gtgccagatt gcacataacg tcgtgattgg
    44221 cgacaatacg gcggttgccg gtggcgtcat tatggcgggc agcctgaaaa ttggtcgtta
    44281 ctgcatgatc ggcggagcca gcgtaatcaa cgggcatatg gaaatatgcg acaaagtgac
    44341 ggttacgggc atgggtatgg tgatgcgtcc catcactgaa ccaggcgtct attcctcagg
    44401 cattccgctg caacccaaca aagtctggcg caaaaccgct gcactggtga tgaacattga
    44461 tgacatgagc aagcgtctga aatcgcttga gcgcaaggtt aatcaacaag actaacgttc
    44521 catcttttgt tcgccaaact ttacggcctg tctcattctt acgattgcgg caggccgtgt
    44581 tattattgtc gtttcttata ttttgacagg aagagtatct tgactactaa cactcatact
    44641 ctgcagattg aagagatttt agaacttctg ccgcaccgtt tcccgttctt actggtggat
    44701 cgcgtgctgg attttgaaga aggtcgtttt ctgcgcgcag taaaaaatgt ctctgtcaat
    44761 gagccattct tccagggcca tttccctgga aaaccgattt tcccgggtgt gctgattctg
    44821 gaagcaatgg cacaggcaac aggtattctg gcgtttaaaa gcgtaggaaa actggaaccg
    44881 ggtgagctgt actacttcgc tggtattgac gaagcgcgct tcaagcgccc ggtcgtgcct
    44941 ggcgatcaaa tgatcatgga agtcactttc gaaaaaacgc gccgcggcct gacccgtttt
    45001 aaaggggttg ctctggtcga tggtaaagta gtttgcgaag caacgatgat gtgtgctcgt
    45061 agccgggagg cctgatacgt gattgataaa tccgcctttg tgcatccaac cgccattgtg
    45121 gaagagggcg cgtcaattgg cgcgaacgca cacattggtc ctttttgtat cgttggaccc
    45181 catgtcgaaa ttggtgaggg taccgtactg aaatctcacg ttgtcgtgaa tggtcatact
    45241 aaaattggcc gcgataatga gatttatcag ttcgcctcca tcggcgaagt taaccaggat
    45301 ctgaaatatg ctggcgaacc gacccgtgtg gaaatcggcg atcgtaaccg cattcgcgaa
    45361 agcgtcacca ttcatcgtgg cacagtccag ggcggtggat tgacgaaggt gggcagcgac
    45421 aacttactga tgatcaacgc gcacattgcg cacgattgta cggtaggtaa ccgctgtatt
    45481 ctcgccaaca acgcaacgct ggcgggtcac gtatcggttg acgacttcgc gatcatcggc
    45541 ggcatgaccg cagtccatca gttctgcatc attggtgcgc acgtgatggt tggcggctgc
    45601 tccggtgtgg cgcaggacgt ccctccttat gtcattgcgc agggtaacca cgcaacgccg
    45661 ttcggtgtca atatcgaagg gctgaagcgc cgcggattca gccgtgaggc gattaccgct
    45721 atccgcaatg cgtataagct gatttatcgt agcggtaaaa cgctcgatga agtgaaaccg
    45781 gaaattgctg aactggcgga aacatatccg gaagtgaaag cctttaccga tttctttgca
    45841 cgctcaacgc gcggtctgat tcgttaatga ctgaacagcg tccattaacg attgccctgg
    45901 tcgccggaga aacctccggc gatatcctgg gggccggttt aatccgcgct ctgaaagaac
    45961 gtgtgcccaa cgcccgcttt gttggtgttg ccgggccacg aatgcaggct gaaggctgcg
    46021 aagcctggta cgaaatggaa gaactggcgg tgatgggcat tgttgaagtg ctcggtcgtc
    46081 tgcgtcgctt actgcatatt cgtgccgatc tgacaaagcg ttttggcgaa ctgaagccag
    46141 atatttttgt tggtattgat gcgcctgact tcaatattac tcttgaaggt aacctcaaaa
    46201 agcagggtat caaaaccatt cattacgtca gtccatccgt ctgggcgtgg cgacagaaac
    46261 gcgttttcaa aataggcaga gccaccgatc tggtgctcgc atttctgcct ttcgaaaaag
    46321 cgttttatga caaatacaat gtaccgtgcc gctttatcgg tcataccatg gctgatgcca
    46381 tgccgttaga tccagataaa aatgccgccc gtgatgtgct ggggatccct cacgatgccc
    46441 actgcctggc gttgctacca gggagccgtg gtgcagaagt tgaaatgctt agtgccgatt
    46501 tcctgaaaac ggcccagctt ttgcgccaga catatccgga tctcgaaatc gtggtgccac
    46561 tggtgaatgc caaacgccgc gagcagtttg aacgcatcaa agctgaagtc gcgccagacc
    46621 tttcagttca tttgctggat gggatgggcc gtgaggcgat ggtcgccagc gatgcggcgc
    46681 tactggcgtc gggtacggca gccctggagt gtatgctggc gaaatgcccg atggtggtgg
    46741 gatatcgcat gaagcctttt accttctggt tggcgaagcg gctggtgaaa actgattatg
    46801 tctcgctgcc aaatctgctg gcgggtagag agttagtcaa agaattattg caggaagagt
    46861 gtgagccgca aaaactggct gcggcgctgt taccgctgtt ggcgaacggg aaaaccagcc
    46921 acgcgatgca cgataccttc cgtgaactgc atcagcagat ccgctgcaat gccgatgagc
    46981 aggcggcaca agccgttctg gagttagcac aatgatcgaa tttgtttatc cgcacacgca
    47041 gctggttgcg ggtgtggatg aagtcggacg cgggccgtta gttggcgcgg tcgtcaccgc
    47101 tgcggtgatc cttgacccgg cgcgcccgat tgccgggctg aatgattcca aaaagctgag
    47161 cgaaaaacgc cgtctggcgc tctatgaaga gatcaaagag aaagcgttga gctggagtct
    47221 gggccgcgcg gaaccccacg aaatcgacga gctgaacatt cttcatgcga ccatgctggc
    47281 gatgcagcgt gccgtcgctg ggctgcatat tgcgccggaa tatgtgttga ttgatggtaa
    47341 ccgctgcccg aaattaccga tgcctgcgat ggctgtggtg aaaggcgata gccgcgtacc
    47401 ggaaatcagt gccgcgtcta tcctggcgaa agtgacgcgt gacgccgaaa tggcggcgct
    47461 ggatattgtt ttcccgcaat atggttttgc ccaacacaaa gggtacccaa ccgcttttca
    47521 tctggaaaaa ctggctgaac acggcgcgac cgaacaccat cggcgcagct ttgggcctgt
    47581 caaacgcgca ctgggacttg cgtcctgatt cttgtgtcga gattaagtaa accggaatct
    47641 gaagatgtct gaaccacgtt tcgtacacct gcgggtgcac agcgactact cgatgatcga
    47701 tggcctggcc aaaaccgcac cgttggtaaa aaaggcggcg gcgttgggta tgccagcact
    47761 ggcgatcacc gatttcacca acctttgtgg tctggtgaag ttctacggag cgggacatgg
    47821 cgcagggatt aagcctatcg tcggggcaga ttttaacgtc cagtgcgacc tgctgggtga
    47881 tgagttaacc cacctgacgg tactggcggc gaacaatacc ggctatcaga atctgacgtt
    47941 gctgatctca aaagcgtatc agcgcgggta cggtgccgcc gggccgatca tcgatcgcga
    48001 ctggcttatc gaattaaacg aagggttgat ccttctttcc ggcggacgca tgggcgacgt
    48061 cggacgcagt cttttgcgtg gtaacagagc gctggtagat gagtgtgtcg cgttttatga
    48121 agaacacttc ccggatcgct attttctcga gctgatccgc accggcaggc cggatgaaga
    48181 aagctatctg cacgcggcgg tggaactggc ggaagcgcgc ggtttgcccg tcgtggcgac
    48241 caacgacgtg cgctttatcg acagcagcga ctttgacgca cacgaaatcc gcgtcgcgat
    48301 ccacgacggc tttaccctcg acgatcctaa acgcccgcgt aactattcgc cgcagcaata
    48361 tatgcgtagc gaagaggaga tgtgtgagct gtttgccgac atccccgaag cccttgccaa
    48421 caccgttgag atcgccaaac gctgtaacgt aaccgtgcgt cttggtgaat acttcctgcc
    48481 gcagttcccg accggggaca tgagcaccga agattatctg gtcaagcgtg caaaagaggg
    48541 cctggaagag cgtctggcct ttttattccc tgacgaggaa gaacgtgtta agcgccgccc
    48601 ggaatatgac gaacgtctgg agactgaact tcaggttatc aaccagatgg gcttcccggg
    48661 ctacttcctc atcgttatgg aatttatcca gtggtcgaaa gataacggcg taccggtagg
    48721 gccaggccgt ggctccggtg cgggttcact ggtggcctac gcgctgaaaa tcaccgacct
    48781 cgatccgctg gaatttgacc tgctgttcga acgtttcctt aacccggaac gtgtctccat
    48841 gcctgacttc gacgttgact tctgtatgga gaaacgcgat caggttatcg agcacgtagc
    48901 ggacatgtac ggtcgtgatg cggtatcgca gatcatcacc ttcggtacaa tggcggcgaa
    48961 agcggtgatc cgcgacgtag gccgcgtgct ggggcatccg tacggctttg tcgatcgtat
    49021 ctcgaaactg atcccgcccg atccggggat gacgctggcg aaagcgtttg aagccgagcc
    49081 gcagctgccg gaaatctacg aagcggatga agaagttaag gcgctgatcg acatggcgcg
    49141 caaactggaa ggggtcaccc gtaacgccgg taagcacgcc ggtggggtgg ttatcgcgcc
    49201 gaccaaaatt accgattttg cgccgcttta ctgcgatgaa gagggcaaac atccggtcac
    49261 ccagtttgat aaaagcgacg ttgaatacgc cggactggtg aagttcgact tccttggttt
    49321 gcgtacgctc accatcatca actgggcgct ggagatgatc aacaagcggc gggcgaagaa
    49381 tggcgagccg ccgctggata tcgctgcgat cccgctggat gataagaaaa gcttcgacat
    49441 gctgcaacgc tcggaaacca cggcggtatt ccagcttgaa tcgcgcggca tgaaggacct
    49501 gatcaagcgt ctacaacctg actgcttcga agatatgatc gccctagtgg cactgttccg
    49561 ccccggtccg ttgcaatcag ggatggtgga taactttatc gaccgtaaac atggtcgtga
    49621 agagatctcc tatccggacg tacagtggca gcatgaaagc ctgaaaccgg tactggagcc
    49681 aacctacggc attatcctgt atcaggaaca ggtcatgcag attgcgcagg tgctttctgg
    49741 ttataccctc ggtggcgcgg atatgctgcg tcgtgcgatg ggtaagaaaa agccggaaga
    49801 gatggctaag caacgttctg tatttgctga aggtgcagaa aagaacggaa tcaacgctga
    49861 actggcgatg aaaatcttcg acctggtgga gaaattcgct ggttacggat ttaacaaatc
    49921 gcactctgcg gcctatgctt tggtgtcata tcaaacgtta tggctgaaag cacactatcc
    49981 tgcggagttt atggcggcgg taatgaccgc cgatatggac aacaccgaga aggtggtggg
    50041 tctggtggat gagtgctggc ggatggggct gaaaatcctg ccaccagata taaactccgg
    50101 tctttaccat ttccacgtca acgacgacgg cgaaatcgtg tatggtattg gcgcgatcaa
    50161 aggggtcggt gaaggtccga ttgaggccat catcgaagcc cgtaataaag gcggctactt
    50221 ccgcgaactg tttgatctct gcgcccgtac cgacaccaaa aagttgaacc gtcgcgtgct
    50281 ggaaaaactg atcatgtccg gggcgtttga ccgtcttggg ccacaccgcg cggcgctgat
    50341 gaactcgctg ggtgatgcgt taaaagcggc agatcaacac gcgaaagcgg aagctatcgg
    50401 tcaggccgat atgttcggcg tgctggccga agagccggaa caaattgaac aatcctacgc
    50461 cagctgccaa ccgtggccgg agcaggtggt attagatggg gaacgtgaaa cgttaggcct
    50521 gtacctgacc ggacacccta tcaaccagta tttaaaagag attgagcgtt atgtcggagg
    50581 cgtaaggctg aaagacatgc acccgacaga acgtggtaaa gtcatcacgg ctgcggggct
    50641 cgttgttgcc gcgcgggtta tggtcaccaa gcgcggcaat cgtatcggta tctgcacgct
    50701 ggatgaccgt tccgggcggc tggaagtgat gttgtttact gacgccctgg ataaatacca
    50761 gcaattgctg gaaaaagacc gcatacttat cgtcagcgga caggtcagct ttgatgactt
    50821 cagcggtggg cttaaaatga ccgctcgcga agtgatggat attgacgaag cccgggaaaa
    50881 atatgctcgc gggcttgcta tctcgctgac ggacaggcaa attgatgacc agcttttaaa
    50941 ccgactccgt cagtctctgg aaccccaccg ctctgggaca attccagtac atctctacta
    51001 tcagagggcg gatgcacgcg cgcggttgcg ttttggcgcg acgtggcgtg tctctccgag
    51061 cgatcgttta ttaaacgatc tccgtggcct cattggttcg gagcaggtgg aactggagtt
    51121 tgactaatac aggaatacta tgagtctgaa tttccttgat tttgaacagc cgattgcaga
    51181 gctggaagcg aaaatcgatt ctctgactgc ggttagccgt caggatgaga aactggatat
    51241 taacatcgat gaagaagtgc atcgtctgcg tgaaaaaagc gtagaactga cgcgtaaaat
    51301 cttcgccgat cttggtgcat ggcagattgc gcaactggca cgccatccac agcgtcctta
    51361 taccctggat tacgttcgcc tggcatttga tgaatttgac gaactggctg gcgaccgcgc
    51421 gtatgcagac gataaagcta tcgtcggtgg tatcgcccgt cttgacggtc gtccggtgat
    51481 gatcattggt catcaaaaag gtcgtgaaac caaagaaaaa attcgccgta actttggtat
    51541 gccagcgcca gaaggttacc gcaaagcact gcgtctgatg caaatggctg aacgctttaa
    51601 gatgcctatc atcaccttta tcgacacccc gggggcttat ccgggcgtgg gcgcagaaga
    51661 acgtggtcag tctgaagcca ttgcacgcaa cctgcgtgaa atgtctcgcc tcggtgtacc
    51721 ggtagtttgt acggttatcg gtgaaggtgg ttctggcggc gcgctggcga ttggcgtggg
    51781 cgataaagtg aatatgctgc aatacagcac ctattccgtt atctcgccgg aaggttgtgc
    51841 gtccattctg tggaagagcg ctgacaaagc gccgctggca gctgaagcga tgggtatcat
    51901 cgctccgcgt ctgaaagaac taaaactgat cgactccatc atcccggaac cgctgggtgg
    51961 tgctcaccgt aacccggaag cgatggcggc atcgttgaaa gcgcaactgc tggcggatct
    52021 ggccgatctc gacgtgttaa gcactgaaga tttaaaaaat cgtcgttatc agcgcctgat
    52081 gagctacggt tacgcgtaat tcgcaaaagt tctgaaaaag ggtcacttcg gtggcccttt
    52141 tttatcgcca cggtttgagc aggctatgat taaggaagga ttttccagga ggaacacatg
    52201 aacatcattg ccattatggg accgcatggc gtcttttata aagatgagcc catcaaagaa
    52261 ctggagtcgg cgctggtggc gcaaggcttt cagattatct ggccacaaaa cagcgttgat
    52321 ttgctgaaat ttatcgaaca taaccctcga atttgcggcg tgatttttga ctgggatgag
    52381 tacagtctcg atttatgcag cgatatcaat caacttaatg aatatctccc gctttatgcc
    52441 ttcatcaaca cccactcgac gatggatgtc agcgtgcagg atatgcggat ggcgctctgg
    52501 ttttttgaat atgcgctggg gcaggcggaa gatatcgcca ttcgtatgcg tcagtacacc
    52561 gacgaatatc ttgataacat tacaccgccg ttcacgaaag ccttgtttac ctacgtcaaa
    52621 gagcggaagt acaccttttg tacgccgggg catatgggcg gcaccgcata tcaaaaaagc
    52681 ccggttggct gcctgtttta tgattttttc ggcgggaata ccctcaaggc tgatgtctct
    52741 atttcggtca ccgagcttgg ttcgttgctc gaccacaccg ggccacacct ggaagcggaa
    52801 gagtacatcg cgcggacttt tggcgcggaa cagagttata tcgttaccaa cggaacatcg
    52861 acgtcgaaca aaattgtggg tatgtacgcc gcgccatccg gcagtacgct gttgatcgac
    52921 cgcaattgtc ataaatcgct ggcgcatctg ttgatgatga acgatgtagt gccagtctgg
    52981 ctgaaaccga cgcgtaatgc gttggggatt cttggtggga tcccgcgccg tgaatttact
    53041 cgcgacagca tcgaagagaa agtcgctgcc accacgcagg cacaatggcc ggttcatgcg
    53101 gtgattacca actccaccta tgatggcttg ctctacaaca ccgactggat caaacagacg
    53161 ctggatgtcc cgtcgatcca cttcgattcc gcctgggtgc cgtacaccca ttttcatccg
    53221 atctaccagg ggaaaagtgg tatgagcggc gagcgtgttg cgggaaaagt gatcttcgaa
    53281 acgcaatcga cccacaaaat gctggcggcg ttatcgcagg cttcgctgat ccacattaaa
    53341 ggcgagtatg acgaagaggc gtttaacgaa gcctttatga tgcataccac cacctcgccc
    53401 agttatccca ttgttgcttc ggttgagacg gcggcggcga tgctgcgtgg taatccgggc
    53461 aaacggctga ttaaccgttc ggtggagcgg gcgctgcatt ttcgcaaaga ggtccagcgg
    53521 ttgcgggaag agtctgacgg ttggtttttc gatatctggc aaccgccgca ggtggatgaa
    53581 gccgaatgct ggcccgttgc gctaggcgaa cagtggcacg gctttaacga tgcggatgcc
    53641 gatcatatgt ttctcgatcc ggttaaagtc actattttga caccggggat ggacgaacag
    53701 ggcaatatga gcgaggaggg gatcccggcg gcgctggtgg caaaattcct cgacgaacgt
    53761 gggatcgtag tagagaaaac cggcccttat aacctgctgt ttctctttag tattggtatc
    53821 gataaaacca aagcaatggg attattgcgt gggttgacgg aatttaaacg ctcttacgat
    53881 ctcaacctgc ggatcaaaaa tatgctaccc gatctctatg ctgaagatcc cgatttctac
    53941 cgcaatatgc gtattcagga tctggcgcaa gggatccata agctgattcg taaacacgat
    54001 cttcccggtt tgatgttgcg ggcattcgat actttgccgg aaatgatcat gacgccacat
    54061 caggcatggc agagacagat taaaggtgaa gtagaaacca ttgcgctgga acaactggtc
    54121 ggtagagtat cggcaaatat gatcctgcct tatccaccgg gcgtaccgct gttgatgcca
    54181 ggagaaatgc tgaccaaaga gagccgcacg gtactcgatt ttctactgat gctttgttcc
    54241 gtagggcaac attaccccgg ttttgaaacg gatattcacg gcgcgaaaca ggacgaagac
    54301 ggcgtttacc gcgtacgagt cctaaaaatg gcgggataac ttgccagagc ggcttccggg
    54361 cgagtaacgt gctgttaaca aataaaggag acgttatgct gggtttaaaa caggttcacc
    54421 atattgcgat tattgcgacg gattatgcgg tgagcaaagc tttctactgc gatattcttg
    54481 gtttcacgct gcaaagcgaa gtctatcgcg aagcgcgcga ctcgtggaaa ggggatttgg
    54541 cgcttaatgg gcaatatgtg attgagcttt tctcttttcc gttcccgccg gagcgcccca
    54601 gccgaccgga agcttgcggc ctgcgtcatc tggcttttag cgttgatgac atcgatgcgg
    54661 cagtggcgca ccttgaaagc cataacgtga agtgtgaagc catccgtgtc gatccataca
    54721 cgcaaaaacg cttcaccttc tttaacgatc cggacgggct gccgttggaa ctgtatgagc
    54781 agtaaggctt gtcatcgccg catttgcccg gtaacgtgcc gggcattgct actgtaaaat
    54841 cgcaccatca tgacactcac gctcaataga caacttctca cctcacgcca gattctggtg
    54901 gcctttagcg gcgggcttga ctccaccgtt ctgctgcatc agttggtgca gtggcggacg
    54961 gaaaatccgg gcgtcactct gcgcgctatt catgtgcatc acggtttaag tgccaatgcc
    55021 gatgcctggg ttacgcattg cgaaaacgtc tgccaacagt ggcaggtgcc gctggtggtc
    55081 gaacgcgtgc aacttgcgca agaaggactg ggcattgagg cccaggcgcg gcaggcgcgt
    55141 tatcaggcat ttgcccgcac cttgttgccc ggtgaagtgc tggtcaccgc gcaacatctc
    55201 gacgatcaat gtgaaacttt tctgctggcg ctaaaacgcg gtagcggccc tgccgggctt
    55261 tcggctatgg cggaagtttc ggagtttgcc ggaacacggc ttatccgccc gttgctcgcc
    55321 cgcacgcggg gagaactgga gcagtgggca ttggcgcatg gtttacgctg gattgaagac
    55381 gaaagtaatc aggacgactc atacgatcgt aactttctgc gcctgcgcgt agtgccgtta
    55441 ttgcagcagc gttggccgca ttttgcggaa gcaacggccc gcagcgccgc actttgtgct
    55501 gaacaagaga gtctgttgga tgaactgctg gcggatgatt tagcacactg ccaaacgtcg
    55561 caggggacgc tgcagattgc gccaatgctg gcgatgagtg atgcccgccg cgcggcgatt
    55621 atccgtcgct ggctggcagg gcagaatgca ccaatgcctt cccgcgacgc gctggtgcgg
    55681 atctggcagg aagtggcgct ggcgcgggaa gatgcctccc cctgtttacg cttgggcgcg
    55741 tttgaaatcc gccgctatca gtcgcaactg tggtggatta aatccgtcac cgggcaaagt
    55801 gaaaccattg tgccgtggca gacgtggcta caaccgctgg aattaccggc ggggctggga
    55861 agcgtacagc ttactgcggg aggcgatatt cgccctccgc gtgcagacga agcggtcagc
    55921 gtgcgtttca aagcggcagg attgctgcat attgtcgggc gcaacggcgg acgtaagctg
    55981 aagaaaatct ggcaagagtt aggcgtgccg ccgtggctac gtgacaccac gccactgctg
    56041 ttttatggcg aaacgctgat tgcggcggca ggggtatttg tgacgcaaga aggtgtggct
    56101 gaaggtgaga atggcgtaag ttttgtctgg cagaaaacgc ttagttaagt gaaagccgga
    56161 taagacgcat caaacgtcgc atccggcgaa agtcaatcag gactcgctca ccaccaccgt
    56221 accgatttcc gggtggctaa agctggtaat tttatccaga cgtagctcac gggtttcgcc
    56281 agcggcctcg acgaccagat actccacatt tttgcgggag actaaatcgc tggcttttgc
    56341 ctgcaatttt tcgccatctt tcagctcaag tgtcagaatt aaatgatgct ggcaggcgag
    56401 ctcaagatta tcgtaatcat cacaattgat tggttgatac gtatcattca ttgacataat
    56461 cgctcaccag taagtttgcc gcagcgtatg ctgctttttc cctgacagcc tcagaaaggg
    56521 cgtcgtcggc agccatttca ttcagcactt tcaaaacgca gcccagcgcg tccggaacgt
    56581 atcctaagtc tccgctggcg atttccgcgt accgcttgcg tattaactca caatattttt
    56641 ccacatgccc tcctgtcagc actctgactt aaccgtggat gcaagtctaa gcctacgaag
    56701 ataaactctg tttcgcaagg tgactatacc acactcatgt ctgcaatatc agcgccgcaa
    56761 ctgcacgtat tccgttacaa tggcctcctg attcgaaagg agttttctta tggcgcttaa
    56821 agcgacaatt tataaagcga cggttaatgt ggccgatctc gaccgcaacc agtttctcga
    56881 tgcctctctg acgctggcgc gccacccttc agaaacccag gagcgtatga tgctgcgctt
    56941 gctggcgtgg ctgaaatatg ctgatgaacg tctgcaattt acccgtggtt tgtgtgccga
    57001 tgatgagccg gaagcgtggc tgcgtaacga tcacctgggc attgatttgt ggattgagct
    57061 ggggctgccg gatgagcggc ggattaagaa agcctgcacc caggccgcag aagtggcgct
    57121 gtttacctat aatagtcggg cggcgcaaat ctggtggcag caaaatcaga gcaaatgtgt
    57181 gcagtttgcc aatctttccg tctggtatct ggacgatgaa caactggcga aagtaagcgc
    57241 ctttgccgat cgtaccatga cgctgcaggc aacgattcag gatggcgtga tctggttatc
    57301 ggacgataag aataatctgg aagttaactt aaccgcctgg caacaacctt catgattgtg
    57361 atttcccgac atgttgctat tcccgatggt gagcttgaga tcaccgccat tcgtgcgcag
    57421 ggcgcgggcg ggcagcatgt taataagacc tcaacggcca ttcatctgcg ttttgacatt
    57481 cgggcgtcca gcctgccaga gtattacaaa gagcgtctac tcgccgccag ccatcatttg
    57541 atcagcagtg atggcgtgat tgtcattaag gcacaggaat accgcagtca ggaactgaac
    57601 cgcgaagccg cgctagcccg gctggtggca gtgattaaag atttaacaac agaacaaaaa
    57661 gcccgacgac ccacgcggcc cacccgtgca tcgaaagagc gcaggctggc atcgaaagca
    57721 caaaaatcaa gcgtgaaggc gatgcgcggc aaagtacgca gcggtcggga ataaaaagaa
    57781 ggaatagatg gtgaaaaaag cgatagtgac agcgatggct gtaatcagcc tctttactct
    57841 gatgggatgt aataaccggg ccgaagtcga tacgctttct ccagcacagg ctgccgaact
    57901 gaaaccgatg ccgcaaagtt ggcgcggcgt gctgccgtgt gctgattgcg aaggaatcga
    57961 aacctctctg ttcctcgaaa aagacggaac atgggtgatg aatgagcgtt atctcggtgc
    58021 tcgtgaagaa ccttcctcct tcgcttccta cggtacatgg gcgcgaaccg ctgacaagct
    58081 ggtattaacc gacagcaaag gtgaaaagtc atattatcgg gcgaaaggcg atgcgctgga
    58141 gatgctcgat cgtgaaggca atccgattga atcgcagttc aactatacgc tggaaccggc
    58201 acaatctagt ttacctatga cgccgatgac cctgcgaggc atgtattttt atatggctga
    58261 tgcggcgacc ttcactgatt gcgcgaccgg aaaacgtttc atggtagcga ataacgcaga
    58321 gctggagcgt agctacctgg ctgcgcgcgg tcacagtgaa aaaccggtgt tactgtcagt
    58381 agaaggtcac tttacgcttg aggctaatcc ggatactggt gcgccaacca aagtattagc
    58441 gcccgatacg gcgggtaaat tttacccgaa taaggattgc agtagtttgg ggcagtaaac
    58501 ctgcttaagg cagaaacaaa cgcaa
//
LOCUS       DAZGOB010000014        58516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGOB010000014 DAZGOB010000000
VERSION     DAZGOB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.014"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            329..1000
                     /locus_tag="ACHLIF_000904"
     CDS             329..1000
                     /locus_tag="ACHLIF_000904"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO9604963.1"
                     /translation="MDKVLDSALPSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1010..2206
                     /gene="yaiP"
                     /locus_tag="ACHLIF_000905"
     CDS             1010..2206
                     /gene="yaiP"
                     /locus_tag="ACHLIF_000905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO9604964.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2184..2765
                     /locus_tag="ACHLIF_000906"
     CDS             2184..2765
                     /locus_tag="ACHLIF_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9604965.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2767..3540
                     /gene="yaiO"
                     /locus_tag="ACHLIF_000907"
     CDS             2767..3540
                     /gene="yaiO"
                     /locus_tag="ACHLIF_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO9604966.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQNFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3726..4001
                     /gene="frmR"
                     /locus_tag="ACHLIF_000908"
     CDS             3726..4001
                     /gene="frmR"
                     /locus_tag="ACHLIF_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO9604967.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSMEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4036..5145
                     /gene="frmA"
                     /locus_tag="ACHLIF_000909"
     CDS             4036..5145
                     /gene="frmA"
                     /locus_tag="ACHLIF_000909"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO9604968.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5238..6071
                     /gene="fghA"
                     /locus_tag="ACHLIF_000910"
     CDS             5238..6071
                     /gene="fghA"
                     /locus_tag="ACHLIF_000910"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO9604969.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6196..6735)
                     /gene="yaiL"
                     /locus_tag="ACHLIF_000911"
     CDS             complement(6196..6735)
                     /gene="yaiL"
                     /locus_tag="ACHLIF_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO9604970.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6837..8048)
                     /gene="mhpT"
                     /locus_tag="ACHLIF_000912"
     CDS             complement(6837..8048)
                     /gene="mhpT"
                     /locus_tag="ACHLIF_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO9604971.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGDKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8320..9333)
                     /gene="mhpE"
                     /locus_tag="ACHLIF_000913"
     CDS             complement(8320..9333)
                     /gene="mhpE"
                     /locus_tag="ACHLIF_000913"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO9604972.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9330..10280)
                     /gene="mhpF"
                     /locus_tag="ACHLIF_000914"
     CDS             complement(9330..10280)
                     /gene="mhpF"
                     /locus_tag="ACHLIF_000914"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO9604973.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ASARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10277..11086)
                     /gene="mhpD"
                     /locus_tag="ACHLIF_000915"
     CDS             complement(10277..11086)
                     /gene="mhpD"
                     /locus_tag="ACHLIF_000915"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO9604974.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11096..11962)
                     /gene="mhpC"
                     /locus_tag="ACHLIF_000916"
     CDS             complement(11096..11962)
                     /gene="mhpC"
                     /locus_tag="ACHLIF_000916"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO9604975.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHQENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11980..12924)
                     /gene="mhpB"
                     /locus_tag="ACHLIF_000917"
     CDS             complement(11980..12924)
                     /gene="mhpB"
                     /locus_tag="ACHLIF_000917"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO9604976.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12926..14590)
                     /gene="mhpA"
                     /locus_tag="ACHLIF_000918"
     CDS             complement(12926..14590)
                     /gene="mhpA"
                     /locus_tag="ACHLIF_000918"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO9604977.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDVL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14781..15614
                     /gene="mhpR"
                     /locus_tag="ACHLIF_000919"
     CDS             14781..15614
                     /gene="mhpR"
                     /locus_tag="ACHLIF_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO9604978.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15691..16773
                     /gene="lacI"
                     /locus_tag="ACHLIF_000920"
     CDS             15691..16773
                     /gene="lacI"
                     /locus_tag="ACHLIF_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO9604979.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16896..19970
                     /gene="lacZ"
                     /locus_tag="ACHLIF_000921"
     CDS             16896..19970
                     /gene="lacZ"
                     /locus_tag="ACHLIF_000921"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO9604980.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            20022..21275
                     /gene="lacY"
                     /locus_tag="ACHLIF_000922"
     CDS             20022..21275
                     /gene="lacY"
                     /locus_tag="ACHLIF_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO9604981.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(21354..22535)
                     /gene="cynX"
                     /locus_tag="ACHLIF_000923"
     CDS             complement(21354..22535)
                     /gene="cynX"
                     /locus_tag="ACHLIF_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO9604982.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(22541..23011)
                     /gene="cynS"
                     /locus_tag="ACHLIF_000924"
     CDS             complement(22541..23011)
                     /gene="cynS"
                     /locus_tag="ACHLIF_000924"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO9604983.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23042..23701)
                     /gene="cynT"
                     /locus_tag="ACHLIF_000925"
     CDS             complement(23042..23701)
                     /gene="cynT"
                     /locus_tag="ACHLIF_000925"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO9604984.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23811..24710
                     /gene="cynR"
                     /locus_tag="ACHLIF_000926"
     CDS             23811..24710
                     /gene="cynR"
                     /locus_tag="ACHLIF_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO9604985.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24750..26033)
                     /gene="codA"
                     /locus_tag="ACHLIF_000927"
     CDS             complement(24750..26033)
                     /gene="codA"
                     /locus_tag="ACHLIF_000927"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO9604986.1"
                     /translation="MSNNALQTIINARLPGKEVLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26023..27282)
                     /gene="codB"
                     /locus_tag="ACHLIF_000928"
     CDS             complement(26023..27282)
                     /gene="codB"
                     /locus_tag="ACHLIF_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO9604987.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27621..29507)
                     /gene="prpE"
                     /locus_tag="ACHLIF_000929"
     CDS             complement(27621..29507)
                     /gene="prpE"
                     /locus_tag="ACHLIF_000929"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO9604988.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29547..30998)
                     /gene="prpD"
                     /locus_tag="ACHLIF_000930"
     CDS             complement(29547..30998)
                     /gene="prpD"
                     /locus_tag="ACHLIF_000930"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO9604989.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31032..32201)
                     /gene="prpC"
                     /locus_tag="ACHLIF_000931"
     CDS             complement(31032..32201)
                     /gene="prpC"
                     /locus_tag="ACHLIF_000931"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO9604990.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASYPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIANRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32246..33136)
                     /gene="prpB"
                     /locus_tag="ACHLIF_000932"
     CDS             complement(32246..33136)
                     /gene="prpB"
                     /locus_tag="ACHLIF_000932"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO9604991.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTIDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            33375..34961
                     /gene="prpR"
                     /locus_tag="ACHLIF_000933"
     CDS             33375..34961
                     /gene="prpR"
                     /locus_tag="ACHLIF_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO9604992.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRLGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35059..35334)
                     /gene="yahO"
                     /locus_tag="ACHLIF_000934"
     CDS             complement(35059..35334)
                     /gene="yahO"
                     /locus_tag="ACHLIF_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO9604993.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35481..36152
                     /gene="yahN"
                     /locus_tag="ACHLIF_000935"
     CDS             35481..36152
                     /gene="yahN"
                     /locus_tag="ACHLIF_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO9604994.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36169..36414)
                     /gene="yahM"
                     /locus_tag="ACHLIF_000936"
     CDS             complement(36169..36414)
                     /gene="yahM"
                     /locus_tag="ACHLIF_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO9604995.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGQSSPMVGIRQIRLIT"
     gene            complement(36826..37641)
                     /gene="yahL"
                     /locus_tag="ACHLIF_000937"
     CDS             complement(36826..37641)
                     /gene="yahL"
                     /locus_tag="ACHLIF_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO9604996.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(37884..38933)
                     /gene="yahK"
                     /locus_tag="ACHLIF_000938"
     CDS             complement(37884..38933)
                     /gene="yahK"
                     /locus_tag="ACHLIF_000938"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO9604997.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(39020..39976)
                     /locus_tag="ACHLIF_000939"
     CDS             complement(39020..39976)
                     /locus_tag="ACHLIF_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9604998.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(39973..40944)
                     /locus_tag="ACHLIF_000940"
     CDS             complement(39973..40944)
                     /locus_tag="ACHLIF_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9604999.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(40937..42421)
                     /locus_tag="ACHLIF_000941"
     CDS             complement(40937..42421)
                     /locus_tag="ACHLIF_000941"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9605000.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42470..43453)
                     /locus_tag="ACHLIF_000942"
     CDS             complement(42470..43453)
                     /locus_tag="ACHLIF_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9605001.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDSASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(43707..44117)
                     /locus_tag="ACHLIF_000943"
     CDS             complement(43707..44117)
                     /locus_tag="ACHLIF_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO9605002.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(44416..45798)
                     /gene="yahJ"
                     /locus_tag="ACHLIF_000944"
     CDS             complement(44416..45798)
                     /gene="yahJ"
                     /locus_tag="ACHLIF_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO9605003.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(45808..46758)
                     /gene="yahI"
                     /locus_tag="ACHLIF_000945"
     CDS             complement(45808..46758)
                     /gene="yahI"
                     /locus_tag="ACHLIF_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO9605004.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(46901..48319)
                     /gene="yahG"
                     /locus_tag="ACHLIF_000946"
     CDS             complement(46901..48319)
                     /gene="yahG"
                     /locus_tag="ACHLIF_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO9605005.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(48319..49866)
                     /gene="fdrA"
                     /locus_tag="ACHLIF_000947"
     CDS             complement(48319..49866)
                     /gene="fdrA"
                     /locus_tag="ACHLIF_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO9605006.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(49856..50719)
                     /gene="yahE"
                     /locus_tag="ACHLIF_000948"
     CDS             complement(49856..50719)
                     /gene="yahE"
                     /locus_tag="ACHLIF_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO9605007.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(50759..51364)
                     /gene="yahD"
                     /locus_tag="ACHLIF_000949"
     CDS             complement(50759..51364)
                     /gene="yahD"
                     /locus_tag="ACHLIF_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO9605008.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELAREQGFEEIAQLLIAAGA"
     gene            51622..52119
                     /gene="yahC"
                     /locus_tag="ACHLIF_000950"
     CDS             51622..52119
                     /gene="yahC"
                     /locus_tag="ACHLIF_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO9605009.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            52211..53143
                     /gene="yahB"
                     /locus_tag="ACHLIF_000951"
     CDS             52211..53143
                     /gene="yahB"
                     /locus_tag="ACHLIF_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9605010.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(53185..54273)
                     /gene="pdeL"
                     /locus_tag="ACHLIF_000952"
     CDS             complement(53185..54273)
                     /gene="pdeL"
                     /locus_tag="ACHLIF_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9605011.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(54415..58398)
                     /gene="ehaA"
                     /locus_tag="ACHLIF_000953"
     CDS             complement(54415..58398)
                     /gene="ehaA"
                     /locus_tag="ACHLIF_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO9605012.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT        13926 a        15887 c        14574 g        14129 t
ORIGIN      
        1 tcaatcactc gcatattaca ttcacataat gtatataaac aatacttgtt cacagaacat
       61 attttttaaa tgcaattaat tgacaaacta aatcacatct aaatacaatg tataaaacaa
      121 aataactata catacaaaac atacaaacac ttaacatatg aattgcataa aaacattacc
      181 cagtcgaacc tccgggtctg aaagcccttg cttttcagtt attagcaaca tttaacattt
      241 ataaattaac atctggctgc gccagagatt acgtctctga gtgcatcgtt gtgcttgtag
      301 tataataaat aaaatggagt gtcattaagt ggataaggtt ttagattcag ccctcccttc
      361 ttcggcaaat aaaagaaagg gtatccttgc tattggcgca catcctgacg atatagaatt
      421 aggctgtggc gcatcgcttg ctcgtcttgc gcaaaaagga atttatatcg cagccgtggt
      481 gatgactacc ggcaactctg gtacagatgg aataatcgat cgccatgaag aatcgcgcaa
      541 cgccctaaag atattagggt gccaccaaac tattcatctt aattttgctg acacccgcgc
      601 tcatttacag ctcaatgata tgatttccgc tctggaagac atcattaaaa atcaaattcc
      661 ttctgatgtt gaaatcatgc gggtatatac catgcatgat gccgaccgcc atcaggatca
      721 tctcgctgtt tatcaagctt caatggttgc ctgccgcact attccacaaa ttctcggcta
      781 cgaaacccca agtacctggc tttcatttat gcctcaggtt tttgaatccg ttaaagaaga
      841 atatttcaca gtcaaacttg ctgcattaaa aaaacataaa agccaggaac gacgtgatta
      901 tatgcgtcat gatcgcctgc gtgcagttgc acaatttcgc gggcaacagg tcaatagcga
      961 tctgggtgaa ggctttgtaa ttcataaaat gattctttga gcaaattgca tgaaaacctg
     1021 gatatttatc tgtatgtcta tagcaatgtt gctatggttt ttaagtacgc taagacgtaa
     1081 acccagtcaa aagaaaggct gtattgacgc cattatacct gcgtataacg aaggcccgtg
     1141 tctggcgcag tcactggata atctactgcg taacccttat ttttgccggg taatttgcgt
     1201 taacgacggc tccacggaca ataccgaagc ggtcatggcg gaagtcaaac gcaaatgggg
     1261 cgaccgcttt gttgccgtca cgcaaaaaaa taccggtaaa ggtggtgcgc tgatgaatgg
     1321 cctcaattat gccacctgcg accaggtttt tttaagtgat gccgacacct atgttccgcc
     1381 cgatcaagac ggaatgggct atatgctggc agaaattgag cgcggtgctg atgccgtagg
     1441 cggcattccc tctactgcgt tgaaaggcgc ggggctgtta ccgcacatcc gcgcgaccgt
     1501 aaagttgccg atgattgtta tgaagcgcac gctacagcag ctcctgggcg gcgcaccgtt
     1561 tattatcagc ggtgcctgcg gaatgttccg tactgatgta ttgcgtaagt tcggtttctc
     1621 ggatcgtact aaagtcgaag accttgatct cacctggaca ttagtggcaa acggctaccg
     1681 tattcggcag gcgaatcgct gcatcgtata cccacaggaa tgcaacagcc cgcgtgagga
     1741 gtggcgtcgc tggcggcgtt ggattgtggg atacgcggtc tgtatgcgcc tgcataaaag
     1801 acttttattt agccgcttcg gtatcttcag tatatttcct atgctgttgg ttgtgcttta
     1861 tggcgttggg atttatctca ctacctggtt taatgaattc atcaccaccg ggccgcatgg
     1921 tgtggtgttg gcaatgtttc cgcttatctg gatcggcgta gtttgtgtta ttggtgcttt
     1981 tagcgcctgg tttcatcgtt gctggttgtt ggtgccttta gcgccgcttt ccgttgtgta
     2041 tgtattatta gcttatgcca tctggattat ttatggactt attgcctttt ttactggacg
     2101 cgaacctcag cgcgacaaac ccacccgcta ttccgcactg gtggaagcgt caaccgctta
     2161 ttcccaacct tctgtcacag gaactgaaaa actatctgaa gcttaatgtt aaagagaaaa
     2221 atattcagat tgcagaccag gtaattattg atgaaactgc aggtgaagtt gttatcggcg
     2281 cgaatacccg tatttgtcat ggtgccgtta ttcagggtcc ggtagtgatt ggcgcaaact
     2341 gcctgatagg taattatgcg tttattcgtc ctggcacaat aatcagcaat ggtgtaaaaa
     2401 ttggttttgc caccgaaatt aaaaatgcgg ttattgaagc ggaagcaacg attggtccgc
     2461 aatgttttat tgccgactcg gtagttgcaa accaggcata tttgggcgca caagtacgta
     2521 ccagtaatca tcgtctggat gaacagcctg tgtctgttcg aactccagag ggaattatcg
     2581 ctaccggatg cgataaatta ggttgttata tcgggcagcg ttcacgcctt ggcgtacaag
     2641 ttataatttt gcctgggcga attatttccc cgaacacgca acttggcccg cgcgtgattg
     2701 tagaacgtaa tttacctgcc ggaacttatt cactccgaca agaacttatc cgtacaggag
     2761 attaaaatga taaaacggac gttattagcg gcggccattt ttagcgcatt gcccgcttat
     2821 gccgggttaa cttccattac cgcgggctac gattttaccg attattctgg cgatcatggc
     2881 aaccgtaatt tagcgtatgc tgaactggtg gcgaaagttg aaaacgcaac gctgcttttt
     2941 aatctttccc aggggcgtcg tgattatgaa actgaacatt tcaatgccac tcgcggtcag
     3001 ggtgcggtct ggtataaatg gaataactgg ctgacaaccc gaacgggtat tgcctttgcg
     3061 gataatacgc cggtctttgc ccgccagaat tttcgtcagg atattaacct ggccctgttg
     3121 ccaaaaacgc ttttcacgac cggttatcgc tacactaaat attacgatga tgtcgaagtc
     3181 gatgcctggc aaggcggcgt atcactctat actggcccgg tgatcaccag ctaccgctat
     3241 acccattatg actccagcga tgcaggtggt agttatagca atatgatttc cgtgcgcctg
     3301 aatgacccgc gcggcactgg ttatacgcaa ctatggctaa gccgcggaac aggcgcttac
     3361 acctatgact ggacgccaga aacacgctac ggcagcatga agagcgtcag tctgcaacgt
     3421 attcaaccgc tgactgagca acttaatctc gggctgacgg caggtaaagt gtggtacgac
     3481 accccaaccg atgattataa cggtctgcaa cttgcagccc atctgacctg gaaattctga
     3541 ttccttctgc cgcccgctat ccggggcggc cttccctgcc gattagcccc cccctttcct
     3601 ctttgttttc cgaccacatt caccggataa attttattct ccagtgttat atactatagg
     3661 ggggtatgca ttgacatgta gtataccccc ctatagtata ttgcgtgcag atgatgaggt
     3721 gcgaaatgcc cagtactccg gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc
     3781 gagggcagat tgatgctctg gaacggtcga tggagggtga tgccgaatgc cgtgccatac
     3841 tccaacagat cgctgccgtt cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa
     3901 gccatatccg ggaaacgttt gaccgaaatg actgctacag ccgcgaagtc agccaatccg
     3961 ttgacgacac tattgaactg gttcgagcct atcttaaata gctgaatcta ttaccatatt
     4021 gaggaagagc gagagatgaa atcacgtgct gccgttgcat ttgctcccgg taaaccgctg
     4081 gaaatcgttg aaattgacgt tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc
     4141 cataccggcg tttgccatac cgacgcattt accctctccg gggatgaccc ggaaggtgta
     4201 ttcccggtgg ttctcggtca cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta
     4261 accagcgtca aacctggcga ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt
     4321 gagttctgtc gttctggcaa aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa
     4381 ggcttgatgc cagacggcac cacccgtttt tcttacaacg ggcagccgct ttatcactac
     4441 atggggtgct ctacattcag tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt
     4501 aatccagaag caaaccatga acacgtctgt ctgctgggct gtggcgtgac caccggtatt
     4561 ggcgcggtgc acaacacagc taaagtccag ccaggtgatt ctgttgccgt gtttggtctt
     4621 ggcgcgattg gtctggcagt ggttcagggc gcgcgtcagg cgaaagcggg acggattatc
     4681 gctatcgata ccaacccgaa gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc
     4741 attaacccga atgactacga caaaccgatt aaagatgtcc tgctggatat caacaaatgg
     4801 ggtatcgacc atacctttga atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa
     4861 agtgcgcacc gcggctgggg tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa
     4921 atctccaccc gtccattcca gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc
     4981 ggcgtgaaag gtcgttccca gttaccgggt atggttgaag atgcgatgaa aggtgatatc
     5041 gatctggaac cgtttgtcac gcataccatg agccttgatg aaattaatga cgccttcgac
     5101 ctgatgcatg aaggcaaatc cattcgaacc gtaattcgtt actgatttcc cgcaggtata
     5161 ccccgtccac ttcagacggg gttcttaata ctctccctgg gcagccgtcc gggggattaa
     5221 ccgtgagata atgactgatg gaactcattg aaaaacatgc cagctttggc ggctggcaaa
     5281 atgtgtatcg gcattattcc caatcactga aatgtgaaat gaatgtcggc gtctatctcc
     5341 caccaaaagc cgcgaatgaa aaattgccgg tgctgtactg gctttcgggc ctgacctgca
     5401 acgagcagaa tttcattact aaatcgggga tgcagcgtta cgcggctgaa cacaacatta
     5461 ttgttgttgc gccggacacc agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg
     5521 atctcgggca aggtgccggg ttttacctga acgcaacgca agcgccgtgg aatgaacatt
     5581 acaaaatgta tgactatatc cgcaacgagc tgccggattt agtgatgcaa cattttccgg
     5641 caacgaccag aaagtctatc tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg
     5701 cgttacgtaa cccagatgaa tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat
     5761 cgcaagtacc gtggggacag caagcctttg ccgcatatct tggtgaaaat aaagatgcct
     5821 ggttgaatta cgatccggtg agtcttattt cacaaggtca acgcgttgcg gaaatcatgg
     5881 ttgatcaggg gttgagtgat gatttttacg cggaacagct gcggactcca aatcttgaaa
     5941 agatctgcca ggagatgaat atcaagacgt taatccgtta tcacgagggt tacgaccaca
     6001 gctattattt tgtctccagt tttattggcg agcatattgc ctaccacgcc aataaactga
     6061 atatgcgttg ataatagtgc acgactgccg gatgcggcgt gaacgcctta tccgcctaca
     6121 tttcgcccgc aggccgtagg catgataaga cgcgcatagc gtcgcatcag gcatctgcgc
     6181 acgacttatc tatgtttacc acatcaaatc atcaggcact ttgaagtcgg catatgggtc
     6241 gtcttcatcc tgctcttcgg cgctgagcgc gctgtgcaac actatactgc tggcatcacg
     6301 ctgagctatt ttatcggcaa cgctggcggg aataatggcg tattcacctt cgctattgtt
     6361 atcgaccaac aagcgagcaa tcgccagacg accattaatc aactgcgcct gtgtgagctt
     6421 atcgacaaaa atcttcttaa tcagattgcc gtcagtgaag ttaaaaccaa tatcgccatt
     6481 ggcaatggtg attcggttca tttcaatgag ctgcttcacc tgagctttat attctttcgc
     6541 caacgcagct tgtttttgtt gctcgctaag ctgtttatca cgctcaagct gtgccttttt
     6601 attttcttct accgccgccc gagcttcacg cgcctgaacg cgcgattttt tcgccgttct
     6661 ctccaccttc gccgcttttt tgctggtgac taatcctgct ttgagcaact gctcttgtaa
     6721 ggtaagtttt gccatctttg tttccaaacc cgctgaataa tttttagcga gtatacctgt
     6781 aaacgccgtg ggtgtattgg gggcaactcc ctttcgcccc tgcttaataa ggcgcgtcag
     6841 gcatcggcac acggctgcat tcttgatctc cgactcatca aaataaacac cgccaaccca
     6901 gcaacaagaa tacccggtgc agaagcggcc attacgccga ccgtgccagt gcctaatgcc
     6961 agcattttcc cggccagtaa cggaccgctc atagccccca gacgccctac cgccacggct
     7021 gttcccacac ctgttgcgcg gatctgcgaa ctgtaaaaca acggtgccag ggcatacaaa
     7081 acgctttgcc cacctgtcgc aaacaacccc gcgacaaatc ccgccagcaa cataccgtta
     7141 aatgacgaca ccgttccaag tgccagcagc gaagctaaca tgccgctata aatcagtagc
     7201 gacatggtca ctggacgcag cttatccatc aatgcgccca acattaacgt cccgcttgcc
     7261 gcacccattt gcagggcaaa catcacccct gccgcctgcg atggctggaa tccttgctcc
     7321 accaaaagca gcggtagcca gttgatcaac atgtagacca ccagcagagt gaagaaataa
     7381 cacaaccaca gcagcagcgt cgcggttgcc gtttctggcg caaataaggc acgcagtggt
     7441 ggcgcagcct gtttatcgcc agcgaaaacc gccgactccg gcagccagcg cattaatagc
     7501 ggcaccagaa tcaacggcac cacaccacct acccaaaaca ccgtttgcca tgctaagttt
     7561 gcccccgcga aacccagtgt cgccgccagc gccgcgccaa tgggaacacc gcaatacatc
     7621 aggctcactg ctgtcccacg aaaacgtgga cccgcggctt cagacgtcag ggcgataaga
     7681 ttcggcaacg ccgcccccag cccgacaccg gtcatcagcc gcgcaaagac cagtgagggg
     7741 aaatcccagg caatcgccgt tgccagcgag aacaaaccaa acagcgcaac tgagccaatc
     7801 aaaatgcgct tgcgaccata acggtccgcc agcattccgc caaccaacgc gccgggtagc
     7861 aaaccgagta ttccggcgct gaatatccag cccatttgca ttttatcgag tgcgaaagcc
     7921 tgggcgatgc cacccgccgc aatgccagcc gcctgaagat ccagcccttc catcagagcg
     7981 accaaaaaac aaagcccgat agtcagcatc aggcgggatg aagatgatga aggggtacga
     8041 gtcgacataa gatgaacctc gttgcgagtg caggtgaatc gcgactgata cattgccagc
     8101 cgcgtaatca gaactattta atgactgcct acgggcgaaa tgtaggccgg acaagacgtt
     8161 tatcgcatcc ggcagtcgtg tgcagatgcc tgatgcgacg ctaacgcgtc ttatcaggcc
     8221 aaattctcac gccacccgta ggccggataa ggtgtttacg ccgcatccgg cagtcgtgct
     8281 gggatgggca tacaaaatgc acgttctcgg cagtcatcat tatttgttgt tgcgcagatc
     8341 cagcgccacg tcaacgatca tatcctcctg gccgccaacc atccggcgtt tgcccagctc
     8401 aacgagaata tccaccgcac ttaagccata acgcgccgcc gccgtttcac agtgacgcag
     8461 gaagctcgag taaacaccag cgtatcccag cgccagcgtt tcgcgatcga ctcgtaccgg
     8521 tcgatcctgc aacggacgca ccaggtcgtc agcggcatcc attaacgcat agagatcggt
     8581 cccatgctgc cagcccagtt tatccgcggc ggcaataaat acttccagcg gcgcgttacc
     8641 tgcgcccgcg cccattcccg cgaggctggc gtcgattcgg tcgcagccct cttccaccgc
     8701 cgcgatagag ttcgccacgc caagactcag gttatggtga gcgtgcatgc cagtttgcgt
     8761 ttctggtttc agctctgctt tcagggcgcg gaaacggtca cggatatcgc tcatgttcat
     8821 cgcaccgcca gaatccacca cataaataca ggtcgcaccg tagccttcca tcagctttgc
     8881 ctgctttgcg agattctccg gcgtggtcat atggctcatc atcagaaaac caacggtgtc
     8941 cattccgagc tcgcgggcat actgaatatg ctgggcggaa acatcagctt cggtacagtg
     9001 cgttgccaca cgaaccaccc gcgcgccagc ctgccaggca tttttcagat cgtgaatagt
     9061 gccgattcct ggcagcaaca acgtcgcgat tttggcgtgc ttcaccacat ccgccgctgc
     9121 ttcaatccat tcaaggtcgc tatgtgcgcc gaaaccatag ttaaagctgg aaccctgcaa
     9181 accgtcgcca tgggccactt caatcgaatc cacgcgggca tcgtccagtg ctttggcaat
     9241 ctggcgaacg ttttccagcg aatactgatg acgaatggcg tgcataccgt cacgcaacgt
     9301 gacgtccgag atataaagtt ttttaccgtt catgccgctt ctcctgcctt gcgcgccagt
     9361 gactgggcca ttttttccgc tgtcgccagc gcactggaag tcataatgtc gaggttgccc
     9421 gcataggcag gcagataatg cgctgcgcct tcgacttcca gccagaccgc tgttttcagt
     9481 ccggagaatt gccccacgcc cggtaaattg accggtttat cctgcgggat aacttcaaac
     9541 tgcacgcgct gtttcaggcg ataacccggt acgtaagcct gcaccgcctc agccatttca
     9601 ttgattgagg cttcgatatc atcttgtgaa gcttcgtcgc tcaatacata caccgtgtca
     9661 cgcatcatca acggtggctc tgctgggtta agcacaataa tcgccttccc ttttgccgcg
     9721 ccgcccacca cttcaatggc tcgggaagtg gtttccgtaa attcatcgat attggcacgc
     9781 gtgccaggtc cggcagattt actggcgata gaagcgataa tttcggcgta atgaacacgc
     9841 gccacgcgtg aaactgccgc caccattgga atggtggcct ggccgccgca ggtgaccatg
     9901 ttgacgttca gttgatcgac gttcgcctcg aggttaacca ccggcacgca gtaagggccg
     9961 atggcagcag gcgtcaggtc aattaagcga atatccggtt tcgcttcgcg taaagcggca
    10021 tcgtttttca catgcgcgcc cgcgctggtc gcatcaaata caatgtcgat atcagcaaat
    10081 tcaggcatgt tcatcagccc gatcacccct tcatgggtgg tggcgacgcc catacgtctg
    10141 gcactcgcca gaccgtcgga ctgaggatca atgccgacca tcaccgccat ctccagatgc
    10201 tgaccgtgac gcaaaatttt aatcatcaga tcggtgccaa tgttgccaga accgataatg
    10261 gcgactttac gcttactcat gacagacttc cttttggggc tgcgcttgaa aatgtcgcag
    10321 caactgaacc tatgccttca atatgggctt caaaacgatc gcccgcattc accgccacca
    10381 tcgggcctaa tgccccggta agaatgatat ctccggcgcg cagcggttca cccagactgg
    10441 ccattttgcg tgccagccag acggcggcat taagcggatg tcccaggcat tcgctgccgc
    10501 gcccgctgga aacctcttcg ttattacgcg tcatcttcat ggcgcagttt ttcaggtcta
    10561 accccgccgg acgttgcgcc ggaccgccga tgacatacac cccgcaggaa gcgttatctg
    10621 ccacggtatc gacaaactga atcgaccagt cgcgaatgcg gctccccacc acttccagcg
    10681 ccggaagtac ccattcaatg gcgttataca gttcgtcgaa ggtgatatca gttgcgggta
    10741 aatcgcggtt caacaccagt gcgatctccg cttcaatgcg gggttgcaga acacgggaaa
    10801 aaggaatgat ttcgttatcg ccataacaca tgtcggcaaa taacgtccca aaatccggtt
    10861 gatcaacgcc cagttgttgt tgcactttcg gatgcgtcag gcccacttta cgccctacca
    10921 cgcgacgccc ctgcacaacg tcatattgca catttatgtg ctgaatggcg taggccgctt
    10981 cagcgttatc gataccaatc agatcgcgca gcggcgcaat cgcttcgccc tgctctgcgg
    11041 cgcggcgtaa atccgccgcc agttgctcaa gagtatgctt cgtcatgacc attccttaag
    11101 ggcgtgcgag gaaattcagc accaattgat tgaaagcgtc ggcatgttcc cactgcgccc
    11161 agtgaccaca gtcgcggaag atatgcagtt cagaaccggc aatgccggac agcagacgca
    11221 gacccgcatc catcggcaca aagcggtcgt tgcgccccca gacaatcagg gtttgcgctt
    11281 tgatttccgc cagacgtggg ccaaaatccg ggaactgttt cggattagct tccaggctct
    11341 taacgaagtt ttcctggtga tcgcggcgcg acagcatatt attcaggcgc gcttcaaaca
    11401 gggcgtcggt caaatcgctg gtatcgaaaa cgaagatatc catcatcagc ttcaggtttt
    11461 cgatagtcgg ctgacgataa agctgattca gtcgcttaat accttcggtt ggcatcggcg
    11521 taaacaaact catgccgccc gtaccgccgc ccatcagcac cagtttgccg acgcgctccg
    11581 gccagttaag ggtgaacgcc acagaactat ggccgcccat cgagttgccc agcaggtgga
    11641 ttttggcgat atccagttga tccaccacgc ttttcaggat tcgtgcatta agatccgatc
    11701 gtgaaccact attaacgatc gaatcgcttt tgccccaacc cggacaatcc agcaggatta
    11761 cccgatagcc cgcctctacc agcggatcga tattgcggct gaagttcgcc cagccagtag
    11821 cacccgggcc ggaaccatgc agcaggacaa cggtttcgtc gccttgtccg cagtcattaa
    11881 aatggatgcg cagcgtttta cccgcttctt ctacattcag aaaacggctg gtggcggctt
    11941 cggtttgtgg ctgataactc atcatcttct cctgcatatt cagttctctg ttctggcgct
    12001 taacgagcca aatccggcaa tccactccgg gattgggcgg taataacgcc cttcgctacg
    12061 ccagttgcca aatgtagaaa tagcggcaaa agcggcgacc caggttttga tttcatgtgt
    12121 cgactttccg gcaatggcgg aaagctcttc gttactgacg gcatccagtt cctgtatgcg
    12181 tccctgctcc agcaaagtca tgaactggtt atcccaaatc gggttgagcg gatgcagagt
    12241 tctctgatcc tcaacaaact tctcagcggc gctaatcacc cgttgctgac gcaattcgcg
    12301 ctcactggcg ggtaaatctt tcccgctccc caacagacgg tcgcgcatat gggcatcggc
    12361 tttcgccagt tcgggcaccg gcggctgatg ggaaagccca ccggaaccca ggaacagcac
    12421 gcgtttattg agagtgctgg tgaaacgtcc aatggcttca cccaacatgc gggtacgctg
    12481 gaaaccgggc agcggcgtgg cgacaccgtt gatgaacaca ggcagaactg gcaccttatc
    12541 cagcccaccg agcaggaact ccagcggctg ggcgaacccg tggtccacct gcatacagta
    12601 agaaacggca agatcgatcc cgctcttcat gacagcatgc gcacaggcct ccgccagctc
    12661 cacaggcacg ggcagctctc ctgccgcact gccgaaatca ccaattgccg tcgctccaac
    12721 gcctaaacag aacggtggca tcacgtcata gaaaaagccg ttgtagtgat ctggcgcaaa
    12781 cagcaccacc agttcagggg agaaagccgc aatacgctcg cgggcgctgg caatcacgcc
    12841 attgacctca tcgagtacct cttgcgccgg gtcgacatac cccaccaacg gcgagtggga
    12901 aagacagtga agataagcgt gcatatcagg ctaccttttc gacagaaacg tcggcatcag
    12961 ggcgggtcag cgtcatcacc gacgccagtt tattcagcgt attgcccagg gtttgcggaa
    13021 tggcggtggc ggcaacaaag cgatccgggc gcatcaccac cagcgaagca ttgtgttgcg
    13081 cgaaccagct acgcagacga ccttgcgtat cgcccacgcg tagcacgccg tcgtggttat
    13141 cctgtgcggt atgaatttgc acttccggca ccacctgaat gaagcgtgtg cccaacgcgc
    13201 gccactgctg gatttgctcg tcgctcatcc cccacagtgg attgcatccc cagccaatta
    13261 ccgcgaagtt cgcgccaatc gcgttatcga gcagcgtcac gtcgccgttt tccagcgtga
    13321 ctttcggctg aataaacatc ttgccgaccg gagagtgctt cgcttcgccc tcacgtacca
    13381 gcgcaccgcc gtaatattgc ggcatcggct tgaagcgcat ttcgaggaag tagcgtttta
    13441 ctggcggcag ataattcagt agccaggaaa cgccgtcacg taacgtaccc tgccagcgtt
    13501 tcggcggagc cagcacgttg cccgccgtca cggacaggtc aatcatcgct ttggcgtgat
    13561 cgcgacgttc ttgttgatag gtatcgagca gcacatcgcg ggctttcccc tggataacca
    13621 acgccagttt ccaggcgagg ttaaaggcgt cgcgcatacc actgttatag ccctgcccct
    13681 gccacaccgg catgatgtgc gcggcatcgc ccgccagcag tacgcgatca atacggaaac
    13741 gttgcgccag tcgcgcgtta tgggtgtaga cacgctggcg aatcaattca acattgtccg
    13801 gattaggcag cactttgctt aacagcttgc gcatattttg cggctcacgc agctgctctt
    13861 cggtttctcc cggcatcacc ataaattcaa agcgacgtac cgcatgaggc agcgcggcag
    13921 aaacatacgg gcgcaccgga tcgcaacaca aatagatatg cggcgtactt aacggatcgt
    13981 tggcgatatc taccacaatc cactgatttg gcgcagtttt accttcaaac ggcacattca
    14041 gagtgcgacg gacaaaactt gctccaccgt cacaggctac cagccactgg gctttgacta
    14101 tttcccgctg cccttctgcc gttttcaggt gcaaggtcac ttcgtcattt tgctggctga
    14161 aggcctccag ctcgcgggaa aacaagcagc gcacattagg aaaacgcgac accccttcca
    14221 gcatcaccgc atcgacctgc ggctgaataa aggcgttacg gcgcggccag ccaaattcat
    14281 cggtcattgg ctgaatatca gcaaaacagc ggcctttcgg ggtgagaaaa cgcatcgcgt
    14341 gccacggcgt agtgtgcggc agaacattct cgaccaggcc aaccgactgc atggtgcgca
    14401 gcgcctcgtc atcaatacca atcgcacgcg ggtagtcgat caacttatcg agtttctcca
    14461 ccaccagcac gtcaatgccc atctgaccga gatagttcgc catcatcagc ccgaccggac
    14521 cggcacctgc gatcgccacc tgaacgctat ggttaacagc aggctggatg tcagggtgtt
    14581 gtattgccat ttcagtacct cacgactcgg acaaaatgcc gttgcgcgca cagtacggcg
    14641 caacttattt tgttaaaaac atgtaaatga ttttttattg tgcgctcagt ataggaaggg
    14701 tgttttcggc tacaatcaaa acatgcccga atgtgcacca ggtgcacaac gttgttttaa
    14761 ctatagaaat gtcaattaat atgcagaaca atgagcagac ggaatacaaa accgtgcgcg
    14821 gcttaacccg cggtctaatg ttattaaata tgttaaataa acttgatggc ggtgccagcg
    14881 tcgggctgct ggcggaactc agcggcctgc atcgcaccac tgtgcggcga ctgctggaga
    14941 cgctgcagga agagggatat gtccgccgta gcccctccga tgatagtttt cgactgacca
    15001 tcaaagtgcg gcaattaagc gaaggatttc gtgacgaaca gtggatttct gcactggcgg
    15061 ccccactgct gggcgatctg ttgcgcgaag tggtatggcc gacagatgtg tccacgctgg
    15121 atgttgatgc aatggtggta cgcgaaacca ctcaccgttt cagccgctta tcctttcacc
    15181 gggcaatggt cgggcgacgt ttgccgcttc tgaaaaccgc ctcgggcctg acctggctgg
    15241 ccttttgccc ggaacaagac cgcaaggaat taatcgaaat gttagcctcc cgccccggtg
    15301 atgactatca actggcacgg gaaccgttaa agctggaagc cattctggcg cgcgcgcgca
    15361 aagagggtta cggacagaac taccgcggct gggatcagga ggagaagatc gcctctatcg
    15421 ccgtaccgct gcgcagtgaa caacgggtga ttggctgtct gaatctggtg tatatggcga
    15481 gcgcaatgac cattgaacag gcagcggaaa agcatcttcc ggcgctacaa cgggtagcaa
    15541 aacagatcga agaaggggtt gaatcgcagg ctattctggt ggccggacgg cgaagcggcg
    15601 tgcatttacg ttgacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgt
    15661 tcggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgctata cgatgtcgca
    15721 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
    15781 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
    15841 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
    15901 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc tgatcaactg
    15961 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
    16021 gtgcacaatc ttctcgcgca acgcgtcagt gggctgctca ttaactatcc gctggatgac
    16081 caggatgcca ctgctgtgga agcagcctgc gctaatgttc cggcgttatt tcttgatgtc
    16141 tctgaccaga ctcccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
    16201 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
    16261 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
    16321 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
    16381 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
    16441 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
    16501 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
    16561 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
    16621 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
    16681 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    16741 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    16801 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtgaaat
    16861 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg
    16921 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacacaactt aatcgccttg
    16981 cggcacatcc ccctttcgcc agctggcgta atagcgaaga agcccgcacc gatcgccctt
    17041 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
    17101 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
    17161 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta
    17221 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
    17281 atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
    17341 cggcgtttca tctgtggtgc aacggtcgct gggtcggtta cggtcaggac agtcgtttgc
    17401 cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
    17461 tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
    17521 ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
    17581 ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
    17641 gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
    17701 ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtagt tatgccgatc
    17761 gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
    17821 tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
    17881 gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
    17941 cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
    18001 atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
    18061 gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
    18121 atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
    18181 atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
    18241 gtaatcaccc gagtgtgatc atctggtcgc tggggaatga gtcaggccac ggcgctaatc
    18301 acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
    18361 gcggcggagc cgacaccttc gcaaccgata ttatttgccc gatgtacgcg cgcgtggatg
    18421 aagaccaacc cttcccggcg gtgccgaaat ggtccatcaa aaaatggctt tcgctgcctg
    18481 gagaactgcg cccactgatc ctttgcgaat atgcccacgc aatgggtaac agtcttggcg
    18541 gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct
    18601 gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggttggctt
    18661 acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgcatgaac ggtctggtct
    18721 ttgccgaccg cacgccgcat ccggcgctga cggaagcaaa ataccagcag cagtttttcc
    18781 agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
    18841 ataacgagct cctgcactgg atggtggcgc tggatggcaa gccgctggca agcggtgaag
    18901 tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgga ctaccgcagc
    18961 cggagagcgc cggacaactc tggcttacgg tacacgtagt gcaaccgaac gcgaccgcat
    19021 ggtcagaagc cggacacatc agcgcctggc agcagtggcg tctggcggaa aacctcagcg
    19081 tgacactccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt
    19141 gcatcgagct gggtaataag cgttggcaat ttaaccgtca gtcaggcctt ctttcacaga
    19201 tgtggattgg cgatgaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
    19261 cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg
    19321 aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgctcggcag
    19381 atacacttgc cgacgcggtg ctgattacga ccgctcacgc gtggcagcat cagggaaaaa
    19441 ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
    19501 ttgatgttga agtagcgagc gatacaccgc atccggcacg gattggcctg acctgccagc
    19561 tggcgcaggt cgcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
    19621 accgccttac tgcggcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
    19681 cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
    19741 cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga
    19801 tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
    19861 gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtgtcg gcggaattcc
    19921 agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg
    19981 gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca
    20041 caaacttttg gatgttcggt ttattctttt tcttttactt ttttatcatg ggagcctact
    20101 tcccgttttt cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta
    20161 ttatttttgc cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt
    20221 ctgacaaact cgggctgcgc aaatacctgc tgtggattat taccggcatg ttagtgatgt
    20281 ttgcgccgtt ctttattttt atcttcgggc cactgttaca atacaacatt ttagtaggat
    20341 cgattgttgg tggtatttat ctaggctttt gttttaacgc cggtgcgcca gcagtagagg
    20401 catttattga gaaagtcagc cgtcgcagta atttcgaatt tggtcgcgcg cggatgtttg
    20461 gctgtgttgg ctgggcgttg tgtgcctcga ttgtcggcat catgttcacc atcaataatc
    20521 agtttgtttt ctggctgggt tctggctgtg cactcatcct cgccgtttta ctctttttcg
    20581 ccaaaacgga tgcgccctct tccgccacgg ttgccaatgc ggtaggtgcc aaccattcgg
    20641 catttagcct taagctggca ctggaactgt tcagacagcc aaaactgtgg tttttgtcac
    20701 tgtatgttat tggcgtttcc tgcacctacg atgtttttga ccaacagttt gctaatttct
    20761 ttacttcgtt ctttgctacc ggtgaacagg gtacgcgggt atttggctac gtaacgacaa
    20821 tgggcgaatt acttaacgcc tcgattatgt tctttgcgcc actgatcatt aatcgcatcg
    20881 gtgggaaaaa cgccctgctg ctggctggca ctattatgtc tgtacgtatt attggctcat
    20941 cgttcgccac ctcagcgctg gaagtggtta ttctgaaaac gctgcatatg tttgaagtac
    21001 cgttcctgct ggtgggctgc tttaaatata ttaccagcca gtttgaagtg cgtttttcag
    21061 cgacgattta tctggtctgt ttctgcttct ttaagcaact ggcgatgatt tttatgtctg
    21121 tactggcggg caatatgtat gaaagcatcg gtttccaggg cgcttatctg gtgctgggtc
    21181 tggtggcgct gggcttcacc ttaatttccg tgttcacgct tagcggcccc ggcccgcttt
    21241 ccctgctgcg tcgtcaggtg aatgaagtcg cttaagcaat taatgtcgga tgcgccactg
    21301 ggcacatccg acaattattt atagctccat catctggcga aacaagcgtc gcatcatacc
    21361 tctttgaccc acagctgcgg aaaacgtgct ggtgcaaaac gcagggttat gatcatcagc
    21421 ccaacgacgc acagcgcatg aaacgcccag tccatcaggt aattgccgct gatactacgc
    21481 agcacgccag aaaaccacgg ggcaagcccg gcgatgataa aaccgatccc ctgcataaac
    21541 gccaccagct tgccagcaat agccggttgc acagagtgat cgagcgccag cagcaaacag
    21601 agcggaaacg cgccgcccag acctaaccca cacaccatcg cccacgatac cggcaattgc
    21661 agcggcaacc agataaagcc gcagaacccc accagttgta acaccagcgc cagcattaac
    21721 agtttgcgcc gatcctgatg gcgagccata gcaggcatca gcaaagctcc tgcggcttgc
    21781 ccaagcgtca tcaatgccag taaggaaccg ctgtactgcg cgctggcacc aatctcaata
    21841 tagaaagcgg gtaaccaggc aatcaggctg gcgtaaccgc cgttaatcag accgaagtaa
    21901 acacccagcg tccacgcgcg gggagtgaat accacgcgaa ccggagtggt tgttgtcttg
    21961 tgggaagagg cgacctcgcg ggcgctttgc caccaccagg caaagagcgc aacaacggca
    22021 ggcagcgccc accaggcgag tgtttgatac caggtttcgc tatgttgaac taaccaggga
    22081 gttatggcgg caccaagtcc accgccgccc atcagagccg cagaccacag ccccatcacc
    22141 agtggcgtgc gttgctgaaa ccgccgttta atcactgaag gcatcaccgc ctgaatgata
    22201 ccgatcccca ccccaccaag cagtgcgctg ctaagcagca gcacactttg ggggtaaagc
    22261 tcacgcatca atgcaccgac ggcaatcagc aacagactga tggcgacact gcgacgttcg
    22321 ctgacatgct gatgaagcca gcttccggcc agcgccagcc cgcccatggt gaccaccgga
    22381 agagcggtca acagggcagc cacgctaaag ctcattccgc tcgcctggcg caattgcggt
    22441 agcagtggcc cgacggaggt gagcagtggt cgcatattaa gaccaatcag caccagaacc
    22501 agcagcatcg tgaacgtctt ttgatgattg cgcatggctg tcagaacggc ttggtcggca
    22561 gatatttacc atctaaggta atgaccgcac gttcgccacc ttccgggtcc gccactttct
    22621 taacgtcgag tttgaagtta atcgcgctga taatgccatc gccaaacttc tcatgaacca
    22681 acgctttcag ggttgtgccg tacacctgca acatttcata gaaacgatac atcgttgggt
    22741 cggttggaat acggtcatca atgcagccac gcagtggaat catctgcaac agtagaatgg
    22801 cgtcttcgtc gagatccagc ttcgccccga ccaggcgggc ggcgtcggca ggaagcgctt
    22861 gctgccccag caaagccgcg gttacgaagg cttctgccag accggtaccg tcggcaatct
    22921 cggcaaatga gagatctttt ttagctttgc tgagcaaaat ggcatcggca agatcaagac
    22981 gaatattgcg gttaatttgt gactgaatca tggtggatct cctgatggtt taaaaataag
    23041 gttacgctgc ggtcggttgg cgtagcggta tggcacaaac gcgaggatta gcggccagtg
    23101 gcacaaactg gcgggttgcg ccgtcaaaag ctgcgatgct gccgctttca atgtcgtaga
    23161 cccagccgtg cagggcgatc cgcccctctt cgagcgccag gcgcaccgat ggatgagttt
    23221 gcaaattagc caactgagca atgacgtttt cacgtaccat cgccgcagct tttgacggta
    23281 aatcggaatg cgggcgcgcc tcattaacga cgcgggctga atcggcataa cgcagccagt
    23341 gggagacggc aggcatatgg tccatgcact gacagctggc aatggcggtc atcgcgccac
    23401 agttggaatg accacaaatc acaatgtcag atacccgaag cgcagcgacg gcatactcca
    23461 ccgaagcaga aacgccaccg ggttccggcc cgtaggaagg gacgatattg cccgcgttgc
    23521 gaataacgaa cagatcgcca ggctcacgtt gcgtcaccag ctcagggacc agacggctgt
    23581 cggagcagga gataaaaagt gtgcgcgggc tttgctgtgt cgccagctgt ttaaacaagg
    23641 cttcccgctt cggaaatgcc tcgcgctgga atttaaggaa tccatcaata atctctttca
    23701 ccgttaacct ctgtctgtct ctggaatgag aggcagacta ctcgccgatt gtcataaggt
    23761 aaaagtctca tttatgatga gttctattgg atttacttat aggttggcga atgctctctc
    23821 gacatatcaa ttattttctt gccgtggctg aacatggcag cttcacccgt gccgccagtg
    23881 cgttgcacgt ctctcagcct gcgctttccc agcagattcg ccagttagag gagagtttag
    23941 gcgtgccgct gtttgaccgt agcgggcgaa cgattcgtct caccgatgca ggagaagtct
    24001 ggcgacagta cgccagccgg gcgttacagg aactgggagc gggtaaacgg gcgattcatg
    24061 atgttgccga tctgacgcga ggatcgctgc gtatcgccgt cacccccacc tttacaagct
    24121 actttatcgg ccctttaatg gcggatttct atgcgcgcta ccccggcatc acgctccagc
    24181 tacaggaaat gtcgcaggag aaaatcgaag atctgctttg ccgcgacgag ctggacgttg
    24241 ggattgcctt cgcgcctgtg cattcgccgg agctggaggc aattccttta ctgacagaaa
    24301 gtttagcgtt agtcgtggcg caacatcatc cgctggctgc ctgtgaacag gtggcgttga
    24361 gtcgcttgca tgatgaaaaa ctggtcctgc tcagcgcgga atttgccacc agagagcaaa
    24421 ttgaccacta ctgcgagaaa gcggggctac atccacaggt ggtcattgag gcgaactcaa
    24481 ttagcgcggt tctggagctg attcgccgca cttccctttc cacattgtta ccagcagcga
    24541 ttgccacaca acatgacggg cttaaagcga tttctcttgc cccgccacta ctggagagaa
    24601 cggcggtttt gttgcggcgg aaaaatagct ggcagacagc cgccgcgaag gcatttttgc
    24661 acatggcgtt ggaggaatgt gcggatgttg gcgagaatga atcacggtag ccactgacac
    24721 aggtcagtgg ctcttcaaca cagaggaaat taccgtttgt aatcgatggc ttctggctgc
    24781 tccagatata cggtggtttg tgccggttgt gtactggcaa tcaccttgcc gccacgtacc
    24841 gaataacgta ccggaacctg acggcgcagc gcatcaaatc cattttcagc cggcaggata
    24901 atcaggtttg cgctgtttcc ggcggcaatg ccgtaatcct gcaaattcaa tgttctggca
    24961 ctgtggtggg tgattaaatt caggccatcg ttaatctgcc cgtagcccat caactggcaa
    25021 acatgcagcc ccatatgcag cacttgcagc atattcgccg ttcccagcgg ataccacgga
    25081 tcgaagacat catcgtgacc aaagcagacg ttaatgccgg actccagcat ctctttaacg
    25141 cgcgtgatgc cgcgacgttt tggatacgta tcgaaacgtc cttgcagatg aatattgacc
    25201 agcgggttgg cgacaaagtt aataccggac attttcagca agcggaacag gcgtgaggta
    25261 tacgccccgt tataggagtg cattgccgtg gtgtggctgg cggtgactcg cgcgcccatg
    25321 ccttcacggt gcgccagggc agcaacggtt tcgacaaagc gcgactgctc gtcatcgatc
    25381 tcatcacagt gaacgtcgat gagacggtcg tatttttgcg ccagggcgaa ggttttatgc
    25441 agcgactcca cgccgtattc acgggtaaat tcaaaatgcg gaatcgcccc cactacatct
    25501 gcccctaagc gtaacgcctc ttccagcaac gcttcaccgt tgggatacga caaaatccct
    25561 tcctgaggga aggcgacaat ttgcagatca atccacggcg cgacttcctg cttcacttcc
    25621 agcattgctt tcagcgcagt tagcgttgca tccgaaacat cgacatgggt acgcacatgc
    25681 tgaatgccgt tggcaatctg ccatttcagc gtttgccagg cgcgttgttt cacatcgtca
    25741 tgggttaata acgctttgcg ctcggcccag cgttcaatgc cttcaaacag cgtgccggac
    25801 tgattccagt tcggttgtcc ggcggtttgc gtggtgtcca ggtgaatatg cggctccaca
    25861 aacggcggta taactaaacc ttgttcggca tccaggctgt tttcagttat gggcatcacg
    25921 ccggattgcg catcaatggc gctgattttt ccgtcctgca gatgaatctg ccacagcacc
    25981 tctttgcctg gtaaccgggc gttaataatt gtttgtaaag cgttattcga cactgttaac
    26041 ctccacatgc gtcattgctg ctgtcgtttt acgattcaaa atcgggttaa ggatcagata
    26101 gctcagcgcg ccacctaata ccgcgttgac cggaacaatt cccggtaacc agtggcctgc
    26161 ggcaatcccc agcgcgaccg ccagaatcgc cacccaattg acactcatca tacgcgtggt
    26221 cgcaaagtgc tcatagcggc gacggttcat cagatagtcg gcgatgatca cgccccctac
    26281 tggaggaata gctgccgaaa ggaaggtcaa ccagccgaca aaattgttat acagccataa
    26341 tgcgcagacc gtaccgataa taccgttgat taccgaaagg gttttgctcg acataccggt
    26401 aatgttggcg aaacctaaac ccgacgcata gagtgcgtta tcgttggtgg tccagatatt
    26461 cagccccagc accacaatcg caggcagcag caggccctga gcaatcatca catcagagat
    26521 atccgccatg cccagtgccg cagcgcccgc tgcaccgaaa ataaacatca acgagttgcc
    26581 gaggaaaaag gccaccatcg ccaccagcac cgccagtttg gcattgcgac caaaccggac
    26641 aaagtcagcg gtgagcgtac ccgcactgat aaatgacccc acaaccagcg ccagcgcgac
    26701 attgaaatct aacggttgtg cgggaacgac cgcttttaat gcgtccaggc cgcccatgcc
    26761 gttaacagcc agccacacgg aataaccgcc caggcaggcg atagccggaa ccgcaatcac
    26821 cgaaagaacc gtcagcgccg aaatgccaaa aaagacggtg acggtcatca gtaaaccgga
    26881 aacggcaatc agcaaattaa tatccagccc ggttgcctta cccaccggaa tggcaaacat
    26941 cgccacaccg acgccaaacc agccaacctg agttccgccc agtagcagtg aaggcagcca
    27001 tgagccttta acaccaaacg agaagcgagc aagaagatga gtggtcaggc cggtttttgc
    27061 gccaatgtaa ccgagaaatg aagtgtaaat accgaggaga agattaccga tgagaactgc
    27121 gaggaagaaa tcatgatagc taagaccggt tccgagagtg ccgccggtcc acatactggc
    27181 ggaaaagaag gttaatccca gcatgacgaa cgtcaatgcc aatacccctt tccgcgccga
    27241 ctgcgggact ggcccctggc taaagttgtt atcttgcgac acgaaattcc ccctttttgc
    27301 tgtttgaaac ccaaaaaatc cgccgtattc tattcatctg aagataaaaa gcaatcgttt
    27361 tcgtggggaa atatattttt tatatgaatg agtgcaaaaa ggtgggggaa aaattatcgg
    27421 tgtaaaaaca gtgggtagtg ggtagatact gataagacgc gtcagcgtcg catcaggcat
    27481 cggcgcgggg tgtcggatgc gacgcgagcg ccttatccga cctacggttc aggcctgtag
    27541 gcataataag acgcggtaag cgtcgcatca ggcatcggcg catggtgtcg gatgcgccac
    27601 gcgacgcatc cgacaatacc ttactcttcc atcgcctggc ggatctgatc caatgacgca
    27661 ggatcatcaa tggtcgtcag atccccagga tcgcgtcctt cgcaaattgc ctggatcgtg
    27721 cggcgcagca tttttccgga tcgcgttttc ggcaattgcg agacaaacca gacgtgcgcc
    27781 gggcgaccaa agttgccaat ctggctgtcc accagcgcca taatcgcctt ctcttgcgaa
    27841 tgcgccacat cacgatcttc cagactatcg ctctctttcg gaatgacaaa cgccaccgcc
    27901 acctgccctt tcagcgcatc tttcacccca accaccgcca cttcggcaac gcccggatgg
    27961 ctggagatac tctcttcaat ctcgcgcgtg cccagacgat gcccggcaac gttaattaca
    28021 tcgtcagtgc gcccgagaat aaagtgataa ccgtcagcgt cacggatgcc ccagtcaaag
    28081 gtggcgtaca ccgggcggga aaacagcgac cagtaagtct tcacaaagcg gtcgtcgtcg
    28141 ccccagatgg tctgaataca gcccggcggc agcggccctt ccaccaccag catccctttc
    28201 tcgttgacgc cacacggttc gccggtgact tcattaagca actgcacgtt atagccatac
    28261 atcggcacgc cggggcttcc cagacgcgtc ggcctgtcgt ccagaccgcg agcaatcgcc
    28321 ataatcggcc agccggattc ggtctgccag tagttgtcga tgaccggcac atccagcgta
    28381 ttgctcaccc aactggcggt cggctcgtcc agcggttctc cagccagata gagcacttcc
    28441 agcgacgaga gatcgtgttt gcgaatttca gcggtaggga attttttcag cacgcgaatg
    28501 gcggtcggcg ctgagaacat ccggctaacc tgatatttct cgacgatttt ccaccacacg
    28561 ccgcagtccg gccaggtcgg caatccttcg taaacgatag tcgccatccc cgccagcagc
    28621 ggcgcgtaaa cgatatacga atgccccacc acccagccga tatccgatgc gcaaaagaac
    28681 acgctgcccg ctttgccgcc aaaaatggtg tccatcgagg tcgccagcgc caccgcatat
    28741 ccgccgacgt cacgctgcac gcctttaggt ttgccggtcg tgccggaagt gtagagaatg
    28801 caggaggttt cgttggattc cagccacgcc accggtaccc gcgcgccgat gtgttgatgg
    28861 cgcaacgacg cgaaatcgac atcccgcccg ctgacgcgcg ccattttcgc cagcccgcga
    28921 tccaccagca aaacatggcg tggctggtgc tgcgcctgac ttatcgcatc gtcgagcaat
    28981 tttttatagg gaatgatttt gccaccgcgc gctccggcat cagccgagac aatcagcacc
    29041 ggtttagcgt catcaattcg cgccgccacg ctgtgcgagg caaaaccacc aaataccacc
    29101 gaatgaatag caccaatgcg cgcgcaggcc agcagagtaa tatgcgcttc ggcaatcatc
    29161 ggcatataca ccagcacccg atcgccgcgc tgcacgccca gtgagcgcaa cattgaggcc
    29221 accgcgttca cttcgtcatg cagctggcga aaggtaaagg tgcgctcttc ttctgtttcc
    29281 gaagagacgg caatcaatgc cagtgcctct ggctgtttct ccagccagcg gtcgatagcg
    29341 ttgtggcaca aattggttcg gccttcacaa aaccaacggg caaacggcgg attgctgtgg
    29401 tcgagcgttt gcgtaaaggg cgtctgccag tcaatacgcc gggcctgctc ggcccagaac
    29461 tgctccggtt cgttaatcga acgctgataa aattcgctaa aagacataat gctctcctgt
    29521 tgaacttacg ccttaccgca gtttacttaa atgacgtaca ggtcgagata ctcattgacc
    29581 ggcatctgtt ccaggcgagt tctgtcgaga gaaacctcca gaatgcgctg ctgttggcga
    29641 gtcgggaact ggcgcgcgag attgattttg aatttatcga ccagtttcgg aataccatcc
    29701 tgacggcggc gagcatgacc aatggggtac tccaccacca cttcttcaaa tcgtgtgccg
    29761 tcggtgaact caagggtaat ggcattggcg atggcgcgtt tttccgggtc gtggtagtca
    29821 gcggtaaatg ccggatcttc aaagcaattg atcttctcgc gcagggcgtc gatgcgttta
    29881 tcttgcgcaa cgttgtcctc gtaatctgcc gccgttaagc gcccgaataa cagcgggatc
    29941 gccaccatgt actgaatgca gtggtcgcgg tcagccgggt tattgagcgg cccctttttg
    30001 tcgatgatgc gaatacaggc ttcgtgggtg cgaatggtca ctttttcgat atccgccgcc
    30061 gtcttgcctg ctgcctgcat ctgttcatag agcgtcatcg ccgcttcaac tgccgtctgg
    30121 gagtggaact ccgccgggaa ggagattttg aacagcacat tctccatgac gtaagaaccg
    30181 tacggacgct ggaagcggaa cgattcacct ttaaaggaga cgtcgtagaa gccccatacc
    30241 ggcgcggtca gggctgacgg gtaacccatt tcgcccgttt tcgccatcag tgccagacgt
    30301 accgcgcggg aagtggcatc gcccgccgcc caggatttac gcgtgccggt gttcggcgca
    30361 tggcgatagg tgcgcagcga ctgaccgtcc acccacgcca gcgaaacggc gttgagaatt
    30421 tcctcgcggg tcaggccgag catttcggcg accacggcgg tggaagccac tttcactaac
    30481 agaacgtggt cgaggccgac gcggttaaag gagttttcca gcgcgatgca gccctgaatt
    30541 tcatgggctt tgatcattgc ggtcagcacc tgtttcatgg tcaacggcgc tttgccgctg
    30601 gcgacagcgt tgcgcgaaag ccagtctgcc gttgccagaa tgccgccgag gttgtcggaa
    30661 ggatgccccc actccgccgc cagccaggta tcgttgaaat cgagccagcg gatcatcgcg
    30721 ccaatgttaa atgccgcctg gacagggtcg agctgaaact gagttccggg gacgcgcacg
    30781 ccgttgggta cgacggtgcc aggaacaatt ggccccagca gttttttaca ggccgggtat
    30841 tcgagagctt ccagaccgca gccgagcgtg tcgagcaggc agtaatgtgc ggtgtcgtag
    30901 gccactttag agctgatttc gtagttcatc acgtaatcga cgatatcaac gatttcacga
    30961 tcaaattccg ggcggatgtt gttgatttga gctgacattg ggtatgtttc cttattgtta
    31021 ttcgtagagg tttactggcg cttatccagc gcgacaaact ggcggtcttc cggtccaaca
    31081 taattggcgg aaggacggat aattttgttg tcctgacgtt gttcaatgat gtgcgccgcc
    31141 cagccggtga cacgggcgat gacaaacagt ggtgtgaaca tctcggtggg aacgcccatc
    31201 atgttgtagg aaacagcaga gaaccagtcg agattgggga acatcttttt gctctcccac
    31261 atcaccgttt ccaggcgatt ggcgatgttg tacatcttca acgagccgcc ttcctgcgag
    31321 agctgcttcg ccacacgttt gatcacctgg tggcgcgggt cggcgatggt ataaaccgga
    31381 tgaccaaaac caatgaccac ttctttgttt tccacccgct tgcggatatc ggcttcggct
    31441 tcgtccggcg tttcgtagcg ttgctggatc tccagcgaca cttcattcgc tccgccgtgt
    31501 ttcggcccgc gcagtgcgcc aatcgcgcca ataatcgcgg aatacatatc agagccagtg
    31561 cccgcaatca cccgactggt aaaggtggag gcgttaaact cgtgttcggc gtacagcact
    31621 agcgaaatat gcatcgcctt ttcccagctt tgcgatggct tttcgccgtg cagcagatgc
    31681 aggaagtgac caccgatgga gtcgtcatcg gtttccggct ggatgcgttc gccgttgtga
    31741 ctgtagtgat accaataaag gagaatcgag ctaagtgatg ccagcagttt gtcggcaata
    31801 tcccgcgcgc cagagacagt gtgcccctct ttttctggca gtgtgcagcc gagcgcggaa
    31861 acgccggtgc gcatgacatc catcgggtac gacgccgccg gtaaggcttc cagcacggta
    31921 cgcacgttag ccggtaaacc gcgcagggct ttcagtttcg ttttgtaagc ggcgagttcg
    31981 tcacgggtcg gcagtttgcc gtggatcagc aggtgcgcca cttcttcaaa ttcgcaatgt
    32041 tccgccagat caagaatatc gtagccacgg taatgcaggt cattgccact tttacccacg
    32101 gtgcagagcg ccgtattgcc cgccggaacg ccagaaagtg ccacagattt tttcggttta
    32161 atgacatggg tactgttttg caggatcgtt gtgtcgctca tgttgtcctc gtcatttgta
    32221 gggtcgagat atttttcgtt gtgttttatt tcacctggct acgggcaaac aggtcgtcga
    32281 gcttcgcttc gtactggtag tagttgatgc tttcgtacag ctcgttgcgg gtttgcatgg
    32341 tatcgatgac gcttttctgc gtgccttcct gacgcaggac gttgtagaca tgctcagcgg
    32401 cgcggttcat ggcgcggaac gctgaaagcg ggtacagcgc cattgcgaca tgggcgctgc
    32461 gtaattcgtc gatggtaaac agcggcgtgg caccaaattc ggtaatgttg gcgaggattg
    32521 gcacctgcac cgcatcggca aactggcggt acatggcgag ttcggtaatc gcctccggga
    32581 acaacatctc ggcacccgct tcaacatagg cctgcgcacg ctcgatcgcc gcatccagcc
    32641 cctctaccgc cagagcatcg gtgcgcgcca tgatcacaaa atcaggatcg gttttcgcat
    32701 ccaccgccgc gcggatccga tccaccatct cttctttcga gacgatcgct ttattcggac
    32761 gatgaccgca gcgtttcgca ccaacctgat cttcaatatg caatcctgcc gcaccggctt
    32821 taatcatcga tttcacggtg cgcgccacgt taaaggccga agaaccaaaa ccgatatccg
    32881 catccaccag cagcggcagc gaacaaacgt cggtgatacg gcgaatatcg gtcagcacgt
    32941 catcaagagt agaaataccg agatcgggca gccccagcga acctgccgcc acgccaccgc
    33001 cagaaagata aattgcctga tatccggcac gctgcgccaa cagcgcatga ttagcgttga
    33061 tggtgccaac aatctgcaat ggattttctt tagtcagtgc agcgcgaaac gctttacctg
    33121 gagagtgtag agacatagcc catcctttgt tatcaacttg ttatttgcgt tgataaagac
    33181 aaagcaaggg gtgtgccaca attgccttaa taaaattaaa tgtttaattt caatttgtta
    33241 gagataaagt catgaactaa tccgcaaata tgcgtttcag ttaacgtttc aggcaatgtt
    33301 tcaccgcgtt tcattgcaac aattatgaaa caagactaaa cccaatattc ggtttcttaa
    33361 ctttgcggtg cgctatggca catccaccac ggcttaatga cgacaaaccg gttatctgga
    33421 cggtatctgt aacgcgcctg ttcgagctgt ttcgcgatat cagcctcgag tttgatcacc
    33481 tggcgaacat tacccctatc cagcttggct ttgaaaaagc ggtgacctac atccacaaga
    33541 aactggcaaa cgaacgctgt gacgccatca tcgcggctgg atctaacggc gcgtacctga
    33601 aaagccgcct gtcagtgcca gttattttga ttaaaccgag cggctacgat gtgttacagg
    33661 cactggcaaa agccggaaaa ctcacctctt ctatcggcgt tgtcacttat caggaaacta
    33721 ttccggcact ggtggcgttt caaaaaacct ttaatttgcg cctcgatcaa cgtagctaca
    33781 ttaccgaaga agacgcacgc gggcagatta acgagctaaa agctaacggc accgaagcgg
    33841 tggtcggcgc ggggctgatt accgatctgg cagaagaagc cggaatgacc ggaattttta
    33901 tctattccgc cgccaccgtg cgccaggcgt tcagcgatgc gctggatatg acgcgcatgt
    33961 cgttacgcca taacactcac gatgccactc gcaacgccct gcgtactcgt tacgtgctgg
    34021 gcgatatgct cggtcaatca ccacagatgg aacaggtgcg gcagactatt ttgctgtatg
    34081 cccgctccag tgcggcggtg ttgattgagg gggaaacggg gacgggcaaa gaactggcag
    34141 cccaggcaat tcatcgggaa tactttgccc gccacgatgc gcgacagggc aaaaagtcgc
    34201 atccgtttgt tgccgtcaac tgcggggcga ttgccgaatc gctgctggaa gcggagctgt
    34261 ttggctatga ggaaggggcg tttaccggct cgcgacgcgg aggtcgcgcc gggctgtttg
    34321 aaatcgccca cggtggcacg ctgtttttgg atgagattgg cgaaatgccg ctacctttgc
    34381 agacccggct gctgcgggtg ctggaagaaa aagaggttac ccgcgtcggc gggcatcagc
    34441 ctgttccggt ggatgtgcgg gtcattagcg ccactcactg caagctggaa gaagatatgc
    34501 ggcaagggca gtttcgccgt gacctgtttt atcggctgag cattttacgc ctgcaactgc
    34561 caccactgcg tgagcgggtg gcggatattc tgccgctggc ggaaagcttt ttgaaagtgt
    34621 ctctggcggc gctctccgcc ccgttttctg ccgcattacg cctgggatta caggcaagcg
    34681 aaaccgtgct ggtgcactac gactggccgg gcaatattcg tgaactgcgc aatatgatgg
    34741 agcgactggc gctattttta agtgtggaac cgacgccgga tttaacgccg caatttttgc
    34801 agctgctact gccggaactg gcgcgcgagt cggcgaaaac tcccgctcca cgcttactga
    34861 caccacaaca ggcactggag aaatttaatg gcgataaaac agcagcggcg aattatttag
    34921 gcatcagccg gacgacgttc tggcggcggc tgaaaagctg aactgccgaa tgcagcgcga
    34981 acgctttatc cggcataaat ttcaatagat gatcaccgat aaacttacgc atcgggcatg
    35041 tttacatagg gttcagaatt acttcttctt ataaatattt gccgtgccgt ggatcttatt
    35101 gtcagtttga ccggaggtca gtaccaacac atcagcccct ttttcatccg cttttttgat
    35161 caaatcttct ttcgcgtctg cagtcgacat ttcattgcta gttgaaatat cacctatttt
    35221 ttcatactgc gattcaactt tttcaaattc cgctttggtc atcaattcag cggcatagac
    35281 attggtaacg gctaacgcta acgcaccgac taacatttta gagattattt tcatattacg
    35341 gctccagagt tgttatcaca ctatcatcca cccacattat tgggttgatt aataattagt
    35401 tcagtcaggg cataaacgcc agtaaccgga aataagacac tttgtgatct ctctcgcacc
    35461 cgattatttt tggtagaaat atgatgaagt tacttcactt atttatggat gaaatcacta
    35521 tggatccttt gcacgccgtt tacctgaccg taggactgtt cgtgattact ttttttaatc
    35581 cgggagccaa tctctttgtg gtagtacaaa ccagcctggc ttccggtcga cgcgcagggg
    35641 tgctgaccgg gctgggcgtg gcgctgggcg atgcatttta ttccgggttg ggtttgtttg
    35701 gtcttgcaac gctaattacg cagtgtgagg agattttttc gcttatcaga atcgtcggcg
    35761 gcgcttatct cttatggttt gcgtggtgca gcatgcgccg ccagtcaaca ccgcaaatga
    35821 gcacactaca acaaccgatt agcgccccct ggtatgtctt ttttcgccgc ggattaatta
    35881 ccgatctctc taacccgcaa accgttttat tttttatcag tattttctca gtaacattaa
    35941 atgccgaaac accaacatgg gcacgtttaa tggcctgggc ggggattgtg ctcgcatcaa
    36001 ttatctggcg agtttttctt agtcaggcgt tttctttgcc cgctgtgcgt cgtgcttatg
    36061 ggcgtatgca acgcgttgcc agtcgggtta ttggtgcaat tattggtgta ttcgcgctac
    36121 gcctgattta cgaaggggtg acgcagcggt gagcagataa tttactctct acgtaatcaa
    36181 cctgatttgc cgaatcccga ccatcggcga tgattgcccc cgtaatgcgt ggtccttatt
    36241 tccgataagt ttacttgagt ctttagcttc ccatttcagc ccactctccc ctgtgtgggc
    36301 ttttttcatt ggtctgaccc gtcctgaata gccaaacaat ggcaatgttc cactttgcag
    36361 ggatgataat aaggggatct gattcagaag agttttgaaa agttggaccg ccatcgaggc
    36421 ctcgaacccc gtaccataca acaatcctgg ttgtcgtctg ctcttcctga tgagctaatg
    36481 gcggtttttg atatgagcct tacagcatat tttctgtcag gcttgagtgc gatgctaagg
    36541 tacagaaaag accccacaca aacatgatgg aattatgatt tttttcatat gaatgtcaac
    36601 ccacacattc acaaaacatc tgcatgagat cattttttat acaccgcata gctgcgaaac
    36661 cactgctggt gcttttttaa gcgaagcgtc gccgatcaca acaaaatatc ggcgaaaaag
    36721 gcggtggaat gccatttggc atacggatat acagatgaaa tgattacctg ctttgcgatt
    36781 cactctctgg tggaataaca acttgcaggt ggaacccttt ctgaactaaa gagtgtagcg
    36841 gattttaacg tgcttattta caggataata tctgtcacct aattcctgca ctctattcga
    36901 tatccattca tttaatcgtg cgtcatctct gtatgttttt acgtccagaa cagagagcaa
    36961 taacatcacc agtgattcag gaaaaacagg gtggatattt tttaagtgta ataataatcc
    37021 atgcgcaagc gcctgcttaa tgggcgcgtc tgtctccgga atagacgaca tttcgcgaag
    37081 ccatttttcc acacgcatac actctttccc tttagggaaa attctcagca aaggcatatg
    37141 gtgttcaaga agaatacatt tgcaatctgt tcgcactgaa tcgataaact gcactaattt
    37201 cttaagcccg gtaaagcgtc cagcctgcac atttgcttta atatcaagaa acgcatcatc
    37261 ctggtcatat acccaaactg tccctttttg attttcaagt atgcgaaaaa caaacttaac
    37321 cgggtgatca gggtaatcca tccagtcata aaatccaggg aaatcattaa actgcacatt
    37381 tatggtgagt gcgttatcgt ctgaccactc acagtctcgt acggcagcca tgagatgaat
    37441 gagtttgtcg tcatcgacta aatcatcacg acaatcataa ggataatcat aatattgttt
    37501 aatcaaaacc ttgaatatat cgacacagga aaccagggag cttttgatat cctctggtaa
    37561 ctgattgttt ttaaccgtat taagtatgct ttgttgcgta tatggcatca aattattgtg
    37621 cggagctttt aatgatatca tcgcgatctc tcttaaataa aaataaacgt cgcagataat
    37681 aaagctctca ttaaaccagg cactatcaga aatcgctcat aactaacgtg tgaagtattg
    37741 tgtactggag ggagttaatt taacaatttg aatttacgtt ataggtttta ctgctacttt
    37801 gatattattt cgctctcctt aactaaaaag ttaagtggag agcgaagtta atatgttaaa
    37861 ccacagcgta tttattaatt ttttcagtct gttagtgtgc gattatcgat aacaaaacga
    37921 tatttcacat caccacgcag cattcgctca taggcttcat taatttgatc ggcccgaatc
    37981 atctctatat cagccacgat gccatgttcg gcgcaaaaat cgagcatctc ctgagtttct
    38041 ggaatgccgc caatcataga accggctatc gcacggcgtt tcatgatcag gttgaaaact
    38101 tccggcgatt tatgcggtgt cgcaggcgca ccaaccagcg tcatggtgcc atcacgcttc
    38161 agcaaggtgg taaaatcgtc gagattatgt ggcgcagcta ctgtattcaa aatgaaatcg
    38221 aaactcttca catgagccgc catctcatcg gcattgcgtg agttaacaac ttcatcggcc
    38281 cccagggctt ttgccgcttc gcgttttgcc tcagaagtgg taaatgccac cacatgtgcc
    38341 cccatcgcgt gggccagctt aatccccata tgtcccaggc cgccgatgcc gaccacgccc
    38401 acttttttac ccggcccggc ctgccagtga cgtagcggcg aatacgtggt gatccctgca
    38461 cacaacaaag gagccaccgc cgccagctgc tcttgcgggt gacgaatacg cagaacatat
    38521 cgctcatgaa cgacgatctg ttgtgagtag ccgcccagag tatggcccgg ttcgtccggc
    38581 gtcggcgagt tataggtgcc ggtcatgtga tcacagtagt tttccagccc gtcttcacac
    38641 tcttcgcaat gtttacaact gtcgacaatg cagccgacac cgaccagatc gcccggcgca
    38701 tgtttttcta cctgatcacc aacggctacc acacgcccca caatttcatg acccggtacg
    38761 caggggtaaa ccgtccccgc ccactcggaa cggacctggt ggagatcgga atggcagacg
    38821 ccacagtaag cgatttcgat tttgacatca ttcggtcccg gttcacgccg tgtgatatcc
    38881 atcggttcaa gtggttgttt agcggaatat gcaccaacag ctttgatctt cattgtgttt
    38941 actcctgatt agctatgtgt atttggcgat gcctgatatg tcatcgcggc aaaacgcgtc
    39001 cattgaatat agccaatatt taaaccaccc gatccagccc caacatgccg cgaacctgaa
    39061 tgaaacagag cagcaacgtt ccccataagg ccatcgtcag gtaggggctt ataccgagaa
    39121 tattgaagcc gctttcaata atttgcagaa gaaacagcgc cagcaccata ccaattatcc
    39181 gcccgctgcc gccgtccgga ttaaccccgc cgagtactgc cgcgaggatt gacaccagca
    39241 agtacgattc gccataagag gccttagccg agttgagttt cgacatcatc aaaaatgccg
    39301 ctaccgcaca aagcaaggcg gaaatgacat aaacccaaat cagcacccga cgagtgttaa
    39361 tgccggagta aagtgttgct cgttcgttag acccaatcag gtaaatggct ttaccgagcg
    39421 gcgttttctc cagcaatatc cacagcccca gtgccactgc ggcaaacaac cacatcggta
    39481 gcggaatacc gaaccattgc gcatgattca accacagcac ccatgaggga taattggcaa
    39541 tggcgctacc acctgtcacc agaatattta tccctttgag caacgtcatc atcccaagcg
    39601 ttgcaagaat tggcgacact cgaataccag caatcaatac gccgttacat aaaccgatta
    39661 tcaccgccgc gccagcgccc gccagtaacg tagcgacaac cgtagcaata cccggtggat
    39721 actgagtcgc cacccacgcc atcaccagtg aacaggcgtt agcagtggct ataattgaca
    39781 agttaatgcc accgcaaagc atggtcaccg ccatagccaa cgccagaatg cccagcacgg
    39841 gcatttgcga agcgacggac tggaaattac ttatcgacca gaaaatctcc ggcatagtaa
    39901 tgctgaacgc ggcaacagtt agcaccagca ggccgatcaa gtaaaactca acgttattac
    39961 gccacgattt tttcatagca gactccggtt ctgatgctga ctccacgccg tggcactaat
    40021 gctggcaacg atgatgatgc cggtgatcaa tgtttgccag taagacgaga ctcccaataa
    40081 atttagcccg ttttgcatca ctgccaacaa caccacgccg agcaaagtac cggttaacgt
    40141 gccgcgcccg ccgagcaaac tagtgccgcc aagcaccacc gcagccagta ctgtcagctc
    40201 ataacccaga agagaatcgg gggcgacagt catcaccgtc cacgactgca ctacacccgc
    40261 agcgccagac atcaatccca tatagccgta gacgaaaagt tgcagtttca gcacgctaaa
    40321 gccgatgcga ctggctgatt cgcggttccc gccaagggca taaattttgc gccctacact
    40381 ggtgaaattc atgataaacg ctgtcagcag caccaccgta atcatggcga tcagcggcag
    40441 accaaggcca tagtcatagc catcagcatc ggtgtacttg aacaacataa ccccctgctc
    40501 aaaccacggc ggaaagtcgt acagccacac acctttactc aaccataaca gcaggccata
    40561 gaaaatattc agcgtcgaga tagtgatgat gatagaaggc acccgtaggc gattaaccag
    40621 taaggcgtta attaagccaa gtaaaatgcc gatgcagccc gccagtgcaa acgcgacagc
    40681 gaagttgcca ccaatttttt gcagcaacag caccatgccg tattgcgcaa tgatggtcat
    40741 tgctggaaac gaaatatcaa ttccaccgga aatcaacacc acaaacaaac cacaggcgag
    40801 aatagtcaac atggcatagt tattggcgag atcgtacaaa ttaccgaatg tcagaaactc
    40861 gtcgctgcgc caggcaaggc caacaaacag caccacaatc agtaatgcca gccagaattc
    40921 atggcgtttt ttcagttcag ccatttacca cctccgcaat cctggcctga gaactgctgt
    40981 caggcaggaa gctatgggtg atctgacctt tttgcattac caaaatccgg tggctttgat
    41041 accacgcttc ttcaatttca tcgcagatca tcaataccgc aatgccatga gccgccagat
    41101 cgctgataat gccgtatatt cctgctttgt tggcgatatc aacaccgact gtcgggctat
    41161 cgagaatcaa cagtcgtggc ccaatcgcca gccatttggc gatcgatact cgctgcgcgt
    41221 taccaccaga aagcgtattc accggaagat gaggatcaga gactttgatg gtcaatgcct
    41281 tgattagcct gtccaccagg tcacgaactt tagtttcact cagaaagccg aagcgatctt
    41341 ttaccttgtg aaaaacagtg ctaatgatgt tgtcttcaat agattgcgcc atcaccaaac
    41401 cgcgcgacat gcgatcttcc gaaacataac caataccgtg gcggatagcg tcctggttac
    41461 tatgcagtgt aaccggctgg ccgttgatga ggatctcgcc agcatccggt cgggtcatac
    41521 caaacagact cagacataat tccgtgcgcc ccgcaccaag caggccaaca atcgatacca
    41581 cttcgcccgc ttccacccgc aagttaatat ttagatattc cccatgacga cttaaattac
    41641 gcacctcaag caccggggtg ctggattttc cctgccacaa ctctcgcacc tgatattcga
    41701 actcctggcc ggtcatcagg aaaccgagtt gttttgtggt catctctgca gccggaaaag
    41761 tcccgaccag ctcaccatct ttcagcactg aaatacggtc ggaaacttcc atcacttctt
    41821 ccagacgatg actgacgaag accacgcaga tcccgcgttc ccgcaattga tgcaccacct
    41881 gtaataaccc ttgcacttcc tgatgggtca gcgaagcggt tggttcatcc atcacgatca
    41941 accgtgcgtc ctgcgccagt gcacggcaaa ttgctaccag ctgacagcgg gcaattgaaa
    42001 gctcagaaac gagcgtatcc agtggcaatg tgacgttaat actggtcatt gccgcctgcg
    42061 ccacctcacg cagacgacgg cggttaacaa acagaccgtg gtgatagtga ttcacggcga
    42121 tattctccca gacactcagg ttaggaaata aagagaggtc ctgataaatc acctgaatcc
    42181 cctgcgccac cgaagcggcg ggtgtcagct ttgaccagtt tttcccacca agcgtaattt
    42241 ccgctccctc atccgggcga taaacaccgg acataatttt aatcaacgtc gatttaccac
    42301 agccgttttg cccggcaagg cagtgcactt cacccgacat aaagtcgaga gaaacatcgc
    42361 gcagcgctcg cgtggcatga aacgttttgt tgatatgacg aagggaaagg aaggtttcca
    42421 taacatcccc tggcaataag aatcggggct ttcgccccga agaaatggat taataaagtg
    42481 agtcgatatt gtctttgttg accagtaata ccttatggaa acggataatt cgcttatcca
    42541 tatctacgtc ggccttaccc agattctgca tttctaatcc aggtttgatc tcttcaccct
    42601 tcagcaacgt gctggcgacg gctgcgagtg cgtaacctgc agaggccgaa tcgtaagtaa
    42661 tcccttcggt gatatcacca cttttaatca gtgatgccgc ctgtgaaggg atcatcatgc
    42721 catagactgc gactttattt ttcgcccgtt tctctttcac cgcacgtccc gcgccaatcg
    42781 gaccgtttga accaaaggag acaaccgctt tcaagtcagg ataggttttc atcaggtcca
    42841 gtgtggtacg acgtgagtca tccacactct cggcaaccgg catgcggcgg gtaacttcat
    42901 gcatatccgg gtaatgctct ttctggtatt tcaccagcaa gtcagcccat aagttatgct
    42961 gcggcacggt caaactaccc acgtaaatca catagccgcc cttgcccccc atgcgtttcg
    43021 ccatatgctc aacatattca gcggcaaatt tttcgttatc aatgatttcg atatcccagt
    43081 tagcacttgg ctgaccgggg gattcgttgg tcagaaccac aattccggca tctcgcgctt
    43141 ttttgaatac cggttccagc acgttggcat cgtttggcac gatagtaatt gcattaacct
    43201 tacgggcgat taaatcctca ataattttaa cttgttgcgg agcatcagta cttgaaggcc
    43261 ccacctgtga cgcattaaca ccaaaggcgt tacccgcctc aaccacacct tcgcccatgc
    43321 gattaaacca cggcatacca tcgactttag agatattcac cacgactttt tccgctgcct
    43381 ggagcggcgc agaaattagc gcagcgccta ataacagcga agacaccata ttgataacaa
    43441 aacgtttatt catcatttca gcctcattgc agggtagtca ggaagtgttc gcccaaatta
    43501 gcgcgctgga gagaataaga attttcgaat atttttatgc agaataaaca gccctatttc
    43561 aggcagctat taaaaatatg gttataaata tggacaaaac gggcagcaac aataagtgtg
    43621 acattcatca cgtaaaaaat agcaaaataa acaaaattag cgatatttta tgtgtgtgag
    43681 tttatttcgc gtagataaat agttatctac acaacgtcaa taagggcata ttcttcaaat
    43741 ataacgtcca tttctggtga atagcttcct tctgcctgga aaaaacgacg gtgttcatta
    43801 cgccaatatt caagactgag gtcaccttca ccttccttac gggcaagctc cacggtgaca
    43861 tcagaaaagc gcagtaaatg caggccaatc actctgataa cacatacagg ctcaccacgg
    43921 ctattttcta caatcttata ctcaccaatc atcggaaatg cgttgtcttc aatacatccc
    43981 gccagcgaac cacaggaagc tgttttaaca ccagtcgcaa tcaacgttgc gaggtgatcg
    44041 gccaacgctg ctgaatcgcc aaaagtacaa aaggaagcat ggggatattt ttgtgagaat
    44101 ttttctttta tggtcatggc aatatttttg cgattacgga aaaacaaatc acatcttaat
    44161 tactgttggt aaccatcagc aagagtatca ttggtttttc gatactgaat cttttttatc
    44221 cgaagcaggt cacatttatg gcctgataaa tacctttgtt aaaactcact tttgtgtatt
    44281 tctctcaaat aatcccctgg aaattatgcc gcgagattat aaagttcagg atcattcatt
    44341 agcaaaccat tcccgcccca ctccctgggg cttaaatctc ttacaattca ccgttgcagg
    44401 cgtttcaaat gcgcatcaac ctgccacact cccccacacc agttgccctt tatggaacgt
    44461 tgcggttctc ggcgagatac gcgccaccgc ctcggcggaa caggaggcgt ccaccagcac
    44521 aaagctggca tcatcctgcg cttttggcca tacacgctcg cctttttcat tcagcggcaa
    44581 tacatcgcca gtggctagaa acagcgaacg ggagaggttc tgttcgttag gacgaatata
    44641 gagctgcgcg tagagattgg ctttttccag catgtcgccc agaccataag gcgaccagtg
    44701 gtcgataacg ctgtcagtgc cagtcatcac ttttacgcct ttgtcgtgca actgtttgag
    44761 cggcatatgc agcgtgccaa tcggcaccgt cgaggcgata gaaatttgct gcgccgccat
    44821 ccgattcgcc agcgcatcga cctgttgttc gttgagcgta gccagcgcaa aggcgtggct
    44881 gatggtcagc ttacctttca gttgcggcgt tttctctacc gtttcaacca tataattgat
    44941 ggctgccacg cccgacggag tggtttcgtg caggtgaata tcgacacctt tgtcgtagtc
    45001 cagcgcaatc tgaaacatgg tgtcgaggga tttttccatc gcgccatcga cactggtcgg
    45061 gtccagcccg ccgacgtaat gcgcacccgc ctgcatcgct tcgcgcatta agggttccga
    45121 tttcgacagc agcaaaccgt gctgcgggaa ggcgacgatt tcacactcga agcccgcctg
    45181 acgtcgcgcc agcaccgcct gcaaattctg cagatttttc aggccggaaa ccggttcgat
    45241 attgcagtgg ctgcgggcaa tggtggtgcc tttcgactgc aatagatcga tcagtttttc
    45301 tgcccgttcc tgcgtgtacg gttgcagttc cggcagcatt ttctgctcaa gcttgatcat
    45361 gtcctggata gtggtgcctg ccggacgatt gagcgagcgc cacggcccgc cgtagaaggt
    45421 tttgtcgaga tgaatatgca tgtcgcgagt ggtgggtagc atcaacttac cgccagcgtc
    45481 atagtgcggc agcgtggcgt ccggatgctg cttgttctcg cgcagggcga caattttgcc
    45541 gtcctgaatc tccacggtct ggcgcgccgt gcgggtctgc accgccacac cgttttcgta
    45601 gtcaaaaccg gtttccagca gcacgttatc gagatagtaa tgcgggtcag tgattttcat
    45661 ggtgttgttc gcttcgcagt ttagggtgcc agctaccgcc gcatgggcga gcggcacaga
    45721 ggtaccaaac agcgcggcgg cggtgaccat cttaccgctc tggctcagaa attcacggcg
    45781 gctattgctt tctttcatcc tacgtcctta cgttttaata atatgggtgc ccgtttcacc
    45841 gcgcagcgcc gcaggcaggc attccggcgt ggtgataatc acttctttgc cgccctgctc
    45901 caggaacgtc aggctggcga tgatttttgg caacatgctg ccgggcggaa aatgcccttc
    45961 ctgcatatag cgagtcatgg tggcaatatc cacccgatcc agcgcctgct gctgcggttt
    46021 accaaagtgg atacagactt tttcgacgcc ggtggtgatc accagaatgt cggcgtgaat
    46081 ttcacgggcc agcagcgcgg tagagagatc tttgtcaata accgcgtcca cgctttggta
    46141 atcccccgct tcagtacgca ctaccggaat tccaccgccg cccgcgccaa ttacgacaaa
    46201 accttgttgg atcagcgcct taatggcggg tgcttcgaca atacgtttcg gttccggcga
    46261 ggcgaccacg cggcgatagc cccgtccggc atcttcaaca aaacaccagt cagggtttgc
    46321 cttttgtagt ttgtcacgct ggctttcact gaagaatgca ccaatgggct tggtgggatg
    46381 ggcaaaacct ggatcgtttt tatccacttc cacctgagtt accacggtga cggctttttt
    46441 ctcgccgtga cgcgccagcc ggttattcag cgcctgttgg atcagatagc cgatgccgcc
    46501 ctgcgtatct gccacacagt tcgccagcgg cgttaagggc agcccttcgc gctcgtgggc
    46561 aatctccgca cggcgtaaat ccagcccgac ctgcggcccg ttaccgtggg tcagcacaat
    46621 gtcgtaatcg gaagccagca tttccaacac cgtatcggcc acggctttca ccgcctccgc
    46681 ctgatgctca atcgactggc tggcgttatc tttgataatg ctgttgccac caatggcaac
    46741 gaccacaagc tctttcatgt tgttatgtcc gctacagagg gtgattaggt tatgaccaac
    46801 gccagactgc ctgatgcgct acgcttatca ggcctacaat tctcgggcca tccgtaggcc
    46861 ggataaggcg ttcacgccgc atccggcatg aacatgtagg tcagacgccg taacgttcgc
    46921 accagccaag aatggctttc tcgaagcagg caaaaggtgg atgcacaatg cccgcgccaa
    46981 tcatgccgac gcccgcatct ttatgggcga tggcagtgtt gatcaccggc agaatgccgc
    47041 tgctacccac gcgggtgatg tcgattgccg acggcacgcc cataaaaccg agcagcggaa
    47101 tggtgacgtt ggggttttca ccgagagtga tttcacgcat ctggcgagag aaatcgatag
    47161 cctcttccac cgtgccgccc accagcgcaa cgattgcagg cgcggtcgcc atagcaaatc
    47221 cgccaatacc gtaggtttca gtgatggcgc tgtcgccgat atccagcccc gaatcttccg
    47281 gcttataacc ggcaaacatc gggccgatga cctgctgcgc cggaccggta aaccattgcc
    47341 ccggtaatcc gctgacccgc aggccgaact cgacgccgtt acgcgccatg gtggtgacca
    47401 cggtgctgta ttcgatgccg tgcgccgcat ccatcgccgc tttacacatc gccatccacg
    47461 tcgggccgga gaagtagtcg ctgctagcga caaactcaaa cacttcgcgc tgtttctcca
    47521 cggaataacc tgcctgaata attcccggcg tcagcgcctg aatcagcaac gtcgtcccgg
    47581 cgttattgcg gttatggcac tcatcgccca tatgcagcgc ctgcgccagc attaagcgca
    47641 gatcaatctc gccgataatt ttcatcgcgt cgcgcagcat tggcccctgc acatcgcgca
    47701 tccagttgag gcggtcgatc acgctctgat cgttggctcc catacgcaaa atcttcgcca
    47761 tctgctcgct catattggtg taagcgatgt tgccgtaggt tttgtttttt acgatgtgca
    47821 taaacatcga ggcagaggta acacccgcca tcgatcccac acagtcgtgt tcgtggcacg
    47881 gcgagaaggt gatctccccg gaggccgcca gttcagccgc ctcgtcgaga tctttcgcca
    47941 gcccttcgaa caccaacgcc ccggtgaccg cgcctttcat cgcgccgcac attttttccc
    48001 aggtgatcgg cggcccggcg tgaagaatgg ttttcggcgt catgcccggc accacgttaa
    48061 tcgcctgatc aaaaccaatc agcaccggat gcgactggat aatgcgctcc agcgcctgct
    48121 ggttagcggc ggcgattttg tctgccagcg gcgaatcggc aatgttatcc agcgcctgca
    48181 ccacctgcat attgccctgc cccggcggcg tccagtcgag ttgagtcact tctacatgct
    48241 gctttttcag gtcatcgcta aacatggcga tgccgacgtt aataacgttc aatggttggc
    48301 taaacagtga ctggctcatc aggcttcctc ccctttgcag ataaattcac gcgccagcaa
    48361 tccggtattg gtgctgctgc tcgccagaat cactccggca tcaagcagca tctggctttg
    48421 ttgttctaac gatggcgtat caagatcggt acccagcaca taggcgagaa tgatcaactc
    48481 gcgtccttcg gcagcggcga tcgctttcgc ttctttgatc gcctcgatgg tggagccgac
    48541 cggatcttca tgcgatccaa atccgagcac aaaatccatc acgatcaccg ccacttctgg
    48601 atcgcgcgcc tcttcgatca agcgactgat gcggttggtg gggtcaatca ttggatgcgg
    48661 cttgccgttc gtgaagtcgt catcgccaaa gtcgaggaag gtgtgtttga tgctgcggtt
    48721 gatatcttgc aggcggaatt ccggatcggg ctgaatgttg ctgtagacat cgccatgttt
    48781 ttccatcacc gcgaacatgg tttcgtcgca cagcgtgccg ccgcagaaca ggccacgaat
    48841 gtatttctgc tgcggttgca gacgcgcacg cacatcagca atcaacggct ggttaagcgt
    48901 atgcaggtcg agattttcct gtttcacgcc ggagagcatc accgctttta gcgccgcctc
    48961 tttgctgccg cgggcaaact gtagcccctg ctcatccacc ggcgtttcaa cgcgaccgag
    49021 gaagcagacc accaccggct tgcggcaggc acgtgcgcgt tccagcactt tgcgggccac
    49081 cgcaggcgcg ggaggttttg aaatcagtgc gataatttca gtttgcggat cgttttccag
    49141 catcccgatg gcgtcgagca tcatcaggcc gccgattttc tcgctcaggt cgcgcccgcc
    49201 ggtgccaatc agttgtgaaa caccgccacc aaattcatga atgcggacgc tcagctcctg
    49261 gctgccggtg ccggatgcgc caacaatacc gatattgccg cgacgcacgg cgttaccaaa
    49321 acagagcgcc gcgccgttaa taatcgccgt gccgcagtcc ggccccatca tcagcagccc
    49381 tttttcgtgg gccaactgct tgagcgccag ttcatcttca actgagacgt tatcggaaaa
    49441 cagcatcacg ttgagatcgt tttgcagcgc ctgacgcgct tcgcgagcgg caaacagacc
    49501 gttgaccgaa atcaccgcca ggttactttc cgggatatgc tttttggcgc tggcaatagt
    49561 ggcgtaacgc gcctcgtgcg agccgctttg cgctttggtg ttgaacagtt cttcaatctc
    49621 caccagcaac tgctcgttgt ccgcacccga tttaccattg atgacaatca tcaggtcgcc
    49681 gtttttcgcc tgttccagct ccggcgtcag cagtcctaaa tttttcagta cacctttgtt
    49741 catttcggtc gccatcgcca caaatgcctg ctcgacgccg tcgagtttat ttgcacgcgt
    49801 ggagatagac atcagcgaga cagaatcaaa ataggtattc ggtttaatga ctattttaac
    49861 tgacataatt ccctccgtag gtttggctca gcgcacaacc atcggccatg ccatacagca
    49921 tgtcgcagcc ggaactggag ccaatatgtt taattttttc tattgcctga gttgcgttcc
    49981 cagggatgtc ataaataatg ttgtgaataa aacgatgaat attttcccgg caacgttgtt
    50041 gtaatgcggc ttccagcgtt atagcactta ataatgtggt gttatttctg ccgcgctgca
    50101 taccgagata aaattcctct ttgtattttt ccgacggatg cccgggaata aataaaataa
    50161 gcgccagacc tgttaaataa tcgtcggctg atggcgtaag gccaatccct aacccaatca
    50221 tactggcgac cgctgcggcg atattttgtt tttcgtttac tgcctgaata actgcccgtc
    50281 gtttaatatg tagttcttta tttaacgcct gataaaacgg attatcgccg cgataattaa
    50341 acagggtttc attttccccc aggtgctgat gaataatgtc gtgccactgt agccaggcga
    50401 taaggtgaaa attcacagcg gtcatttgca aggttgggga cagccaacgc cgacaacgac
    50461 tcatctctat atgaagatgt tgaccaaccg taataccttg atcgtcaaac tgaaccttat
    50521 caccatgccg gaacagatca tcaaagtgag tgagtgccaa ccgacaactg tttggcgcat
    50581 tgtcgtactc ttcacaaagc agcgtcagca actgctggcg agccgggagt gcgatattta
    50641 ccgctctggc aaagacctgt tcaacctgtc cttgcccccg ctgcgccaga aaatccgcat
    50701 ccgcagttag cgcccacaca caccgtcttt ctggtgtgtt gatagcaagc ctcccggttt
    50761 atgcacctgc ggcaatcagt aactgcgcaa tctcttcaaa gccctgttcc cgcgccagtt
    50821 ccagtggcgt tttgccatat ttatcggtca gatgcgggct ggcaccgtgt tccagcaata
    50881 actgcacaat cgcctgctgt ttaataccac catcattaag cacaatcgct tccagtagcg
    50941 gcgtccagcc tacatggttg gtctggttaa cgttaatctc cgtatgcgcc aaaagctctt
    51001 ttacaatact taaatggcct ttttcacagg caggcgtcag gccgacaccg ccaaaacggg
    51061 ttacgcaatt aagatcgggt ttagccggta aaataattcg tagtagcgtt aaatcatcgt
    51121 tcagacagct aattaaaaaa ggatttaaac aggtatgatc ttgcttatta atatccgctc
    51181 cggcatcaat taatgcctga acgcaagcat attgctgata taaacttgcc agcgtaattg
    51241 ccgttttccc ctgacgatcg caggtattaa tatcgacacc cagcgcaagg caggttttta
    51301 ctttatcgat atcaccctgt tgtgcagcca ataaatagtc ggcaggtaga ttttttatac
    51361 tcattcacat tcccctggtt tttgtggctg ttttcgatga attgagaata gcaacgataa
    51421 tctgataaaa gaacccgctt aaatttaatt cgctgtattt cgcctttttg ttgaatgatg
    51481 ttcaacaatc ttttcgcagt gtgcggtagt gcaatatttg tttctttgac ccgataccct
    51541 ttcactcaat caggttttat ttctctatag tctgcacatg taagagtagc tttcgtccat
    51601 gtctaaccag aggggaacaa gatgaacgga ctcacggcaa cgggtgtcac cgtcggtatt
    51661 tgtgcagggc tttggcagtt agtgtcttca catgtcgggc tctctcaggg atgggaatta
    51721 ttaggaacca ttggcttcgt cgccttttgc agtttttatg ccgcaggcgg cggtaaatcg
    51781 gggtttatca gaagtctggc ggttaattat tccgggatgg tgtgggcatt tttcgccgcg
    51841 ctggctgccg gttggctggc ctctgtaagt ggactgtccg cgttctgggc gagcgtaatt
    51901 actaccgtac ccttctcggc ggtagtggtc tggcaagggc gtttctggtt actgtccttt
    51961 attcccggtg gctttctcgg catgacgctg ttttttgcca gcgggatgaa ctggacggtg
    52021 acgttactgg gttttctggc gggtaactgc gtggggataa tttcagaata tggtggacaa
    52081 aaattaagcg aagcgacgac taaacgcgac gggtattaaa agttattcaa aaatgattat
    52141 ctatctttaa ccgatacctc tacagtcata gctggagaaa ttcatagtag ataatggcga
    52201 gaatggttta atgaactcaa tttttaccga agagaatctg ttggcattta ctaccgctgc
    52261 gcgttttggc agcttcagta aagccgcaga agagttgggt ttaaccactt ccgccattag
    52321 ctacaccatc aagcgtatgg agacggggct ggatgtggtg ctgttcactc gcagtacccg
    52381 cagcattgag ttaacggagt ccgggcgtta ttttttccgc aaagctaccg acctgctgaa
    52441 tgatttttat gccatcaaac gcagtatcga tactatttcg cagggcatag aagcgcgtgt
    52501 gcgcatttgt attaatcagc tgctttatac gcccaaacac accgcacgct tgttacaggt
    52561 gctgaaaaag cagtttccta cctgccagat taccgtcacc accgaagtgt ataacggcgt
    52621 ctgggatgcg attattaata atcaggccaa tatcgccatc ggcgcacccg atacactact
    52681 ggacggcggc ggtattgatt acactgaaat tggcgcgatt cgctgggctt ttgctatcgc
    52741 cccggatcac ccgttagcct ttgtgccgga acccatcgcc gaaagccaac tgcgtctcta
    52801 ccctaatatt atggtggagg acaccgcgca tacgattaat aaaaaagtgg gctggttgct
    52861 gcacgggcag gagtcaattc tggtgccaga tttcaacacc aaatgtcagt gccagatttt
    52921 aggtgaagga attggttttt tgccggatta catggtccgt gaggcgatga cgcaatccct
    52981 gctggttacc cgacaaatcc ataatccgcg ccaggattcg cgcatgttac tggcgactca
    53041 gcattctgct acgggtcagg tgacgcagtg gattaaaaaa caatttgcgc caaatggcat
    53101 attaacgggg atctatcagg atttactgca tcgggagaat taatcgctgc caacctcccg
    53161 atgcgctgtg aatagcggtt tacatcaacc acctgctttc attacccatt cagagataaa
    53221 tttattaccc ggtactggcg gagagtacaa atagccctgc aaaaagtgaa cgcctttacc
    53281 gatcattaaa tccgcctgct cctgggtttc taccccttcc gccacgatac tcagaccagg
    53341 cttacgcgct agttcgacaa tattgtccac aatatgaccg gagatttcgt cgacactcgc
    53401 catttgcaca aatgacttat cgatcttaat aaaatcgacc gggaacgcct gcaagtaacg
    53461 ataggtcgca taacccgtac caaagtcatc cagcgcaaag gtaatgttgt gctgatgaag
    53521 gctgtcaaat atcgctctgg cttctggcgt taccggaata gggttacgtt ccgttagctc
    53581 gagaaccagc ttgattttat cgttacctaa tttcttaacc aggttcagac actctttttc
    53641 aaatcccgct gccaaaaaac aacccgccga gacgttgatg ccaatatgga aattgtccgg
    53701 cagcaaatgt tttaccggca tcagaatatc cgcagtctgt ttcatcagtt ggcgggtcat
    53761 tatgacaata agaccggatg actccgccag aggaataaac tgatccggtg ggataattcc
    53821 cgtttgtgga tgttcccagc ggacaagtac ctcacagccc gtcagtaccc cagtctgcgc
    53881 gcagaaaacc ggttggatcc acggtttgaa ttcgtggttt tccaacgcca gactgatggc
    53941 ttcaggcgtg acgatatggt gacaacggtt atcagggata taaaaattat ttttatttcc
    54001 cgtgccactg atgacttgcg gatgatattg aaaaaagata tcccgccaga aggtaatatc
    54061 actctggatg cctagttttt catacagctg cttcttttga tgtgaaattg tctttgcact
    54121 gcgatttctg tactgtgaga tttctgtaac cgacattccc tccattagca gcttgagtaa
    54181 aagccgttct ttttcggaca cgataccagg caacgtcaaa ttcaccgttg ccccgaactc
    54241 ttccaaaaaa acactcaaat catgcgaagc cattgaacac accttgatta aatcttgtgc
    54301 gccagctaaa tctgcgagtc ttcactctga ctgataaagc tggctgcaag aaaatgacac
    54361 agagagccga attatctccc gttcggctct ctctcctttt actcattatt cagatcagaa
    54421 ttgccactta atgccaacca ttgcagaggt gtcattatat cccctatcgg caacctgaac
    54481 gcccacattc ccccagacat tcaggttggc atttagctgt ccttccaccc cggtttttat
    54541 ctcagcaata tttcgggctc catcctgagt gacggacacg ccatccatac tggtgctgaa
    54601 atccttactg ttatgcagcc agttcacttc tacaaacggc tggaactcgc gggatttacc
    54661 gtcatccatt ttatggtggc tcttcagcca ggtttttacg ccaagtcggg tttgaacatt
    54721 gccatcaccg ttgctatgaa cgagggttcc gttgctttcg cggtgcttat cggctttgac
    54781 tcccatccag gtaacctgtg cttgcggctg aacataccat tcattacgcg ttccctggct
    54841 gccattaaat tcagctaatt tgtgtttgta tccagcttcc agtgaagcgg taaatccttt
    54901 tgatttatag gattcacttt gtaagtcatc ccctttcact gtgttatcaa accagctgta
    54961 ctgcgcccaa ctgtcgagat acgcgccatt acgcgactcg tcatcggcat accaggtggc
    55021 atagaggccg gtgctatatc cgttcacact cgcttttgca cgataaccgg ttcgcgagga
    55081 aatggttttg ctgtcgctgt tgccatatcc cgccatgacc ccaacatgcc agctgtcgct
    55141 gccgttttgg ctccactgcg cgacatcgcc tcccagttgc agaacatagc gattgctttg
    55201 ggttttcagc tggccgctgc catcacgcca tttattatga ccaccttcat ggcgcatcca
    55261 catagtggtt tgtttctgct cacccgtcac catgtcggta tagtacgtat tacccagacg
    55321 ctcatgcaga cgcgtggtga acattgtatt cgctgctgca aggttcgcaa tgtagctacc
    55381 cgcctccggt cgcaggtcat tatccacatt cagatccgga cccggcgtag gtgtcgggtt
    55441 agggttcggc tctggattcg ggtttggctc cggattcggc atcgggtctg gctccggctg
    55501 cagttcagga gaatcactgc cgctggtcag ataccagtta ccactatttg ccccttgtcc
    55561 acgcgcgaga gtgtagtcat acgcccccgc aacgatacga ccagcctgaa caaattcgcc
    55621 ctcagactta ccgtctacat ggataagttc gataccgtta agggttttcg cacctgtacc
    55681 gccagcgtta ttcaccgtaa cggcagtcgt gccgctagta tcgccctcga caaccaattt
    55741 atcggttact gagtcatcgc cattcagtac cgtattcatt actagttgac cgttattacc
    55801 gtggtagttc cccttcacca ctaacacatt gccagcttgc tgtcctgaca ttccggtcat
    55861 cagcgtaccg ttgttggtca gattgccgcc cagggtgtaa ttcacagacg cagccgctct
    55921 tttttcagtg ctattattca acgccagggt accgttgttc tcaacgtttg cgctacccaa
    55981 tccctgcgca tgacttacca ggacattagc cgaattagca atgttcacat tcgccgtcag
    56041 gttgctgtta tcgcccgcga tgtccagcgt gccgccctga atattcagct ctccgctccc
    56101 cgttaacgaa ccggttgaag tgcccccgtt ggtaaccgtc agactgccgc catttaagct
    56161 cagcagtgaa tcggcgctgc cattgagcgt gcccacggtc tggctgtgac cattcatatc
    56221 cagttcagtc tctgccgcca gacgcagttc atgggtatta cccaacacgt tatcgttatt
    56281 caacaaaagc gtcccactgc gcagatcggt aacaccggta tagtcgttgt ctttgttaga
    56341 aagcgatacg gtctgaccct tctggctgct gaatgccagg tcaccactgc cggtaatctt
    56401 tgcgctgaga tcggcggcat tctcgctttt accgttagag ctcagcacca gagcatcgct
    56461 gtcggtagcg tgtaaatcaa gctgggtcag gccgtaaccg atatacagac cgtcattgtt
    56521 taccccactc gtcagacgat agtcgtaagt gccgacggcc gctttttgcc cattctgctg
    56581 aatatcacgt tgggcgctgc tggagaggat ttgcccattt ttatcctgca attgcagatt
    56641 gcccgcatcg cccagaactt ccgcaccttg cgcatcaacc agcttaatgg cgctgttacc
    56701 atcatcgact tcagtgagcg ataacgcaga gtcaatatca cgagatactg agcggacaac
    56761 gtcactgtca gaaacctgaa tcacaccctc gccgcgcaaa tccagcgttt tactaacgtt
    56821 gaccgtccct tctgtcatct gtgcacctgc tgtcagggca ccaaaatcaa cggttccgcc
    56881 ggagaatccc aaagtgctaa tggagtgagc gcccttaccc actgttacct ggcttccctg
    56941 acccgcagtg atcccgcctc tctgcagaaa ctcagcattc ttttccaggt taaatgtcgt
    57001 gttctggaac gccagtgaac cctggaacca gtctgtataa gcaaactcat cgaaggagaa
    57061 ttcgtgattc cccatgttta tttcaacaat acctctacct tgaagatcgt tatcaaatac
    57121 ccaatcttta ttggcattga tttgcagttt gccatcaaca tccaggtccg cacggccgat
    57181 gttcttgtct tcagaaataa ccgcgacagc accagaagcg acatcaatat atccgctgaa
    57241 tgcatagtta ttgccggtca gtgccactgt cccactgtcg atacttattg taccattgcc
    57301 ggaaatctta ttggcaaagg tcatgctatc gtcagccttc aggtgtaatt tcccgcggtt
    57361 gttgatctct gctgtaccta tatttttggt gatccctaaa gcgccatttt gctcaatttt
    57421 gactttacca ctaaaggcag agttatcacc ggttaagtaa gtcgtgccat tgagcgcatt
    57481 gatatagccc aggccctcgc tgccactgac aatatccgcg gaaatagtcg cgtttttatc
    57541 cgtgtggttt aaggtgattt tcttatcgga tgtctttgcc cacacaaact caattttttc
    57601 ggcatcaata atccctgcag ccttcgcagc acttccttcc tgtgcaccta acgctaactc
    57661 agagttggtg cttaaactaa ttttcggtgc ggatatttta cctccatctg atacgactaa
    57721 tgttgtatca gaacgttggt cgtaataacc caggctaata tgttcacgag cattaacttc
    57781 accaccgtta ttaaccgaca ggaactgatt accgccatat ccgccgtaaa cgcccataaa
    57841 gaaggtccct gcatttacct gactaccttc gccgcttaca ttcccctgcg tataagtcag
    57901 acgactggtg cccttaacat gaattttgcc gccattggag acattaaaat agccttgatc
    57961 atgtctcatg cctgagtttg actcatacaa atcatcagca tataactcgc tgccagcatt
    58021 ggagacattc aggaaccccg tacctttcag caaaaagtta agcgggccat tgattgttac
    58081 ctttcctccg gtaatatcca acccaccacc agaagcgaga gtcagacgcg gacgggaagt
    58141 atctgtcgta ttgattacca gattggcatt gtcagtcacc gtaatacggt aatcgatgct
    58201 ctggttctct ttgccaaaat caatagtttt atcctggttt acgatgacat catcggcgaa
    58261 taccagagat gaatataaac ttatcgcgct aattaaaaca gcccgggact tttttccttt
    58321 tactctgctc cccagctcag agcagacgct ccaacagtta cgggacctgt tccactttag
    58381 ccgatatatt ttattcatca caatctccag caatacatcc aaataaaacc aagattgcat
    58441 aaacagcaac gcattaaatg ccattctggt tactgttcat cgcatataaa aattcaaaaa
    58501 ttttgcatag cattgg
//
LOCUS       DAZGOB010000015        56910 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGOB010000015 DAZGOB010000000
VERSION     DAZGOB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.015"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            43..963
                     /locus_tag="ACHLIF_000954"
     CDS             43..963
                     /locus_tag="ACHLIF_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9605013.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVSYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            963..1895
                     /locus_tag="ACHLIF_000955"
     CDS             963..1895
                     /locus_tag="ACHLIF_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO9605014.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQKALEKVIPFFNTHLG"
     gene            1913..2551
                     /locus_tag="ACHLIF_000956"
     CDS             1913..2551
                     /locus_tag="ACHLIF_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9605015.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVSALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            2860..3588
                     /gene="mpaA"
                     /locus_tag="ACHLIF_000957"
     CDS             2860..3588
                     /gene="mpaA"
                     /locus_tag="ACHLIF_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO9605016.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(3563..4528)
                     /gene="ycjG"
                     /locus_tag="ACHLIF_000958"
     CDS             complement(3563..4528)
                     /gene="ycjG"
                     /locus_tag="ACHLIF_000958"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO9605017.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            4647..5153
                     /gene="tpx"
                     /locus_tag="ACHLIF_000959"
     CDS             4647..5153
                     /gene="tpx"
                     /locus_tag="ACHLIF_000959"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO9605018.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(5197..6738)
                     /gene="tyrR"
                     /locus_tag="ACHLIF_000960"
     CDS             complement(5197..6738)
                     /gene="tyrR"
                     /locus_tag="ACHLIF_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO9605019.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(6886..7947)
                     /gene="ycjF"
                     /locus_tag="ACHLIF_000961"
     CDS             complement(6886..7947)
                     /gene="ycjF"
                     /locus_tag="ACHLIF_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO9605020.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(7944..9341)
                     /gene="ycjX"
                     /locus_tag="ACHLIF_000962"
     CDS             complement(7944..9341)
                     /gene="ycjX"
                     /locus_tag="ACHLIF_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO9605021.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            9496..10494
                     /gene="ycjW"
                     /locus_tag="ACHLIF_000963"
     CDS             9496..10494
                     /gene="ycjW"
                     /locus_tag="ACHLIF_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9605022.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKAEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(10605..11510)
                     /gene="ompG"
                     /locus_tag="ACHLIF_000964"
     CDS             complement(10605..11510)
                     /gene="ompG"
                     /locus_tag="ACHLIF_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO9605023.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11555..12637)
                     /locus_tag="ACHLIF_000965"
     CDS             complement(11555..12637)
                     /locus_tag="ACHLIF_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9605024.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(12651..13310)
                     /gene="pgmB"
                     /locus_tag="ACHLIF_000966"
     CDS             complement(12651..13310)
                     /gene="pgmB"
                     /locus_tag="ACHLIF_000966"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO9605025.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(13307..15574)
                     /gene="ycjT"
                     /locus_tag="ACHLIF_000967"
     CDS             complement(13307..15574)
                     /gene="ycjT"
                     /locus_tag="ACHLIF_000967"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO9605026.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(15571..16626)
                     /gene="ycjS"
                     /locus_tag="ACHLIF_000968"
     CDS             complement(15571..16626)
                     /gene="ycjS"
                     /locus_tag="ACHLIF_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9605027.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(16636..17424)
                     /gene="ycjR"
                     /locus_tag="ACHLIF_000969"
     CDS             complement(16636..17424)
                     /gene="ycjR"
                     /locus_tag="ACHLIF_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO9605028.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEEPAQAYRDSLAWLRTC"
     gene            complement(17442..18494)
                     /gene="ycjQ"
                     /locus_tag="ACHLIF_000970"
     CDS             complement(17442..18494)
                     /gene="ycjQ"
                     /locus_tag="ACHLIF_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO9605029.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPFSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNLIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(18525..19367)
                     /gene="ycjP"
                     /locus_tag="ACHLIF_000971"
     CDS             complement(18525..19367)
                     /gene="ycjP"
                     /locus_tag="ACHLIF_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9605030.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(19354..20235)
                     /gene="ycjO"
                     /locus_tag="ACHLIF_000972"
     CDS             complement(19354..20235)
                     /gene="ycjO"
                     /locus_tag="ACHLIF_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9605031.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(20256..21548)
                     /gene="ycjN"
                     /locus_tag="ACHLIF_000973"
     CDS             complement(20256..21548)
                     /gene="ycjN"
                     /locus_tag="ACHLIF_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9605032.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(21562..23241)
                     /gene="ycjM"
                     /locus_tag="ACHLIF_000974"
                     /pseudo
     CDS             complement(21562..23241)
                     /gene="ycjM"
                     /locus_tag="ACHLIF_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(23453..23767)
                     /gene="pspE"
                     /locus_tag="ACHLIF_000975"
     CDS             complement(23453..23767)
                     /gene="pspE"
                     /locus_tag="ACHLIF_000975"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO9605033.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(23842..24063)
                     /gene="pspD"
                     /locus_tag="ACHLIF_000976"
     CDS             complement(23842..24063)
                     /gene="pspD"
                     /locus_tag="ACHLIF_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO9605034.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(24072..24431)
                     /gene="pspC"
                     /locus_tag="ACHLIF_000977"
     CDS             complement(24072..24431)
                     /gene="pspC"
                     /locus_tag="ACHLIF_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO9605035.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(24431..24655)
                     /gene="pspB"
                     /locus_tag="ACHLIF_000978"
     CDS             complement(24431..24655)
                     /gene="pspB"
                     /locus_tag="ACHLIF_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO9605036.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(24709..25377)
                     /gene="pspA"
                     /locus_tag="ACHLIF_000979"
     CDS             complement(24709..25377)
                     /gene="pspA"
                     /locus_tag="ACHLIF_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO9605037.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            25544..26521
                     /gene="pspF"
                     /locus_tag="ACHLIF_000980"
     CDS             25544..26521
                     /gene="pspF"
                     /locus_tag="ACHLIF_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO9605038.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(26641..27906)
                     /gene="puuE"
                     /locus_tag="ACHLIF_000981"
     CDS             complement(26641..27906)
                     /gene="puuE"
                     /locus_tag="ACHLIF_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO9605039.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(27944..29224)
                     /gene="puuB"
                     /locus_tag="ACHLIF_000982"
     CDS             complement(27944..29224)
                     /gene="puuB"
                     /locus_tag="ACHLIF_000982"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO9605040.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(29226..30713)
                     /gene="puuC"
                     /locus_tag="ACHLIF_000983"
     CDS             complement(29226..30713)
                     /gene="puuC"
                     /locus_tag="ACHLIF_000983"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO9605041.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLTDLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(30988..31545)
                     /gene="puuR"
                     /locus_tag="ACHLIF_000984"
     CDS             complement(30988..31545)
                     /gene="puuR"
                     /locus_tag="ACHLIF_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO9605042.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(31572..32336)
                     /gene="puuD"
                     /locus_tag="ACHLIF_000985"
     CDS             complement(31572..32336)
                     /gene="puuD"
                     /locus_tag="ACHLIF_000985"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO9605043.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            32548..33966
                     /gene="puuA"
                     /locus_tag="ACHLIF_000986"
     CDS             32548..33966
                     /gene="puuA"
                     /locus_tag="ACHLIF_000986"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO9605044.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            34115..34279
                     /locus_tag="ACHLIF_000987"
     CDS             34115..34279
                     /locus_tag="ACHLIF_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605045.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            34269..35654
                     /gene="puuP"
                     /locus_tag="ACHLIF_000988"
     CDS             34269..35654
                     /gene="puuP"
                     /locus_tag="ACHLIF_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO9605046.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALFVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            35788..36033
                     /gene="ymjA"
                     /locus_tag="ACHLIF_000989"
     CDS             35788..36033
                     /gene="ymjA"
                     /locus_tag="ACHLIF_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO9605047.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            36346..37989
                     /gene="sapA"
                     /locus_tag="ACHLIF_000990"
     CDS             36346..37989
                     /gene="sapA"
                     /locus_tag="ACHLIF_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO9605048.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            37986..38951
                     /gene="sapB"
                     /locus_tag="ACHLIF_000991"
     CDS             37986..38951
                     /gene="sapB"
                     /locus_tag="ACHLIF_000991"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO9605049.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            38938..39828
                     /gene="sapC"
                     /locus_tag="ACHLIF_000992"
     CDS             38938..39828
                     /gene="sapC"
                     /locus_tag="ACHLIF_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO9605050.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            39828..40820
                     /gene="sapD"
                     /locus_tag="ACHLIF_000993"
     CDS             39828..40820
                     /gene="sapD"
                     /locus_tag="ACHLIF_000993"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO9605051.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            40822..41628
                     /gene="sapF"
                     /locus_tag="ACHLIF_000994"
     CDS             40822..41628
                     /gene="sapF"
                     /locus_tag="ACHLIF_000994"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO9605052.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            41696..42049
                     /gene="ycjD"
                     /locus_tag="ACHLIF_000995"
     CDS             41696..42049
                     /gene="ycjD"
                     /locus_tag="ACHLIF_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO9605053.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            42417..43205
                     /gene="fabI"
                     /locus_tag="ACHLIF_000996"
     CDS             42417..43205
                     /gene="fabI"
                     /locus_tag="ACHLIF_000996"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO9605054.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            43350..44477
                     /gene="yciW"
                     /locus_tag="ACHLIF_000997"
     CDS             43350..44477
                     /gene="yciW"
                     /locus_tag="ACHLIF_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO9605055.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            44545..46479
                     /gene="rnb"
                     /locus_tag="ACHLIF_000998"
     CDS             44545..46479
                     /gene="rnb"
                     /locus_tag="ACHLIF_000998"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO9605056.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATAMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(46557..46661)
                     /locus_tag="ACHLIF_000999"
     CDS             complement(46557..46661)
                     /locus_tag="ACHLIF_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605057.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            46715..48700
                     /gene="pdeR"
                     /locus_tag="ACHLIF_001000"
     CDS             46715..48700
                     /gene="pdeR"
                     /locus_tag="ACHLIF_001000"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO9605058.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWREKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(48682..48810)
                     /gene="ymiD"
                     /locus_tag="ACHLIF_001001"
     CDS             complement(48682..48810)
                     /gene="ymiD"
                     /locus_tag="ACHLIF_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO9605059.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            48848..49021
                     /gene="yciZ"
                     /locus_tag="ACHLIF_001002"
     CDS             48848..49021
                     /gene="yciZ"
                     /locus_tag="ACHLIF_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO9605060.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            49111..49860
                     /gene="yciT"
                     /locus_tag="ACHLIF_001003"
     CDS             49111..49860
                     /gene="yciT"
                     /locus_tag="ACHLIF_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO9605061.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            50129..50347
                     /gene="osmB"
                     /locus_tag="ACHLIF_001004"
     CDS             50129..50347
                     /gene="osmB"
                     /locus_tag="ACHLIF_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO9605062.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(50473..50799)
                     /gene="yciH"
                     /locus_tag="ACHLIF_001005"
     CDS             complement(50473..50799)
                     /gene="yciH"
                     /locus_tag="ACHLIF_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO9605063.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(50799..51536)
                     /gene="pyrF"
                     /locus_tag="ACHLIF_001006"
     CDS             complement(50799..51536)
                     /gene="pyrF"
                     /locus_tag="ACHLIF_001006"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO9605064.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(51728..52897)
                     /gene="lapB"
                     /locus_tag="ACHLIF_001007"
     CDS             complement(51728..52897)
                     /gene="lapB"
                     /locus_tag="ACHLIF_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO9605065.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(52904..53212)
                     /gene="lapA"
                     /locus_tag="ACHLIF_001008"
     CDS             complement(52904..53212)
                     /gene="lapA"
                     /locus_tag="ACHLIF_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO9605066.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(53361..54125)
                     /gene="pgpB"
                     /locus_tag="ACHLIF_001009"
     CDS             complement(53361..54125)
                     /gene="pgpB"
                     /locus_tag="ACHLIF_001009"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO9605067.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            54295..54885
                     /gene="ribA"
                     /locus_tag="ACHLIF_001010"
     CDS             54295..54885
                     /gene="ribA"
                     /locus_tag="ACHLIF_001010"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO9605068.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(54949..>56910)
                     /gene="acnA"
                     /locus_tag="ACHLIF_001011"
     CDS             complement(54949..>56910)
                     /gene="acnA"
                     /locus_tag="ACHLIF_001011"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO9605069.1"
                     /translation="GQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVG
                     KFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELV
                     EKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNE
                     LEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVT
                     LGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDP
                     IETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEP
                     IGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDT
                     YGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSP
                     AGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSD
                     VVSIYDAAMRYKKEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRS
                     NLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVP
                     CRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        13622 a        15069 c        14185 g        14034 t
ORIGIN      
        1 caccaaccgt taaaacgaac cgacaactcc atgaggaacg gaatgatgaa taataaagtc
       61 agcttcacta acagcaataa tccaaccatc tctttgtctg cagtgagcta tttccctcca
      121 aaatttgatg aaacccgtca gtatcaggca attgtggtat ctcacccagg tgggggcgtt
      181 aaagaacaaa cggccggaac ctatgccaaa aaactggcgg aaaagggatt tgtcactatt
      241 gcttatgacg catcttatca aggtgaaagt ggcggcgagc cgcgtcagtt agaaaaccca
      301 tacattcgta ctgaagacat cagtgccgtg attgattacc tgacaacgct ttcttatgtc
      361 gacaataccc gaattggtgc gatgggaatt tgtgccggtg caggatatac agccaatgct
      421 gcaattcagg atcgccgcat caaggccatc ggtaccgtta gtgcggtcaa tattggttcg
      481 atattccgta atggttggga aaacaatgtg aaatctattg atgccctgcc atacgttgaa
      541 gcgggttcaa acgccagaac cagcgatatt agcagcggag aatacgccat tatgccactg
      601 gcaccaatga aggagtccga tgcgccgaac gaagaactgc gtcaggcctg ggagtattac
      661 cacacccctc gcgcgcagta tccaacagca ccgggttacg ctactttgcg cagccttaac
      721 cagattatta cctatgatgc ttaccatatg gcggaagtgt acctgactca gccgacgcag
      781 attgtggcgg gcagccaggc agggagcaaa tggatgagtg acgacctgta cgatcgagca
      841 tcaagccagg ataaacgcta tcacattgtt gaaggcgcaa accatatgga tctttatgac
      901 ggcaaagcct atgtggctga agcaatttca gtattagctc cgttctttga ggaaactctg
      961 taatgaaaca tattcaaatc cgcaatagtg atatggcctg gcatattgct gccaatattc
     1021 agtttccacc aaattttgat gagtcaaaac agtatccggc gattatcagc gtccatcctt
     1081 ttggcagttg taaggagcag acgtctggca atatctatgg caaagcattg gctgaaaaag
     1141 gctatctcgt tctcgcttat gacgccagtt tccagggaga atctggtggg gagcctcgct
     1201 ggatagaaga tccgacacag cgcgtagagg atattagccg tgttatcgat tatgcagtaa
     1261 ctctgccata tgttaatgct gaacgtattg gcgtgctggg agtgtgtggg ggtggtggct
     1321 atgccattaa tgccgcgctg acagaaaaac gaattaaagc agtagtgagt attacgggtg
     1381 ttaatatcgg gcgtcttttt cgggaaggtt tcagcaatta cgatccgatt ggcgcattga
     1441 atgcaatggc gtcgcaacga accaaagagg ctcgtggtgg agaattacag atcaacgaat
     1501 tacttcccgc cagcctggat gcagcaaaag cccatggact gaccgaacgc gatgtttatg
     1561 aagcaactga ctactataaa acaccacgcg ggcaacaacc gggaggtgcg acaaaaatgt
     1621 tgttctcaca tgcacagaaa acgctggcct gggatgcctt cgcatttact gaagtgttac
     1681 tgactcagcc ggtgatggta gtagttggtg aaaaagtcgg tgccttcggt gcttatcgtg
     1741 atggactgga agtctatggc cgggcgatgg tttcgcagga tcgtcagtta gtttcattac
     1801 ccgatttctc acattatgaa ctgtatgaca aaccggaagc agttcagaag gcgctggaaa
     1861 aagttattcc ttttttcaac acccatctgg gataagtttc atgagaaata ttatgaaaaa
     1921 tgtactgatt cttggtgctg gcggtcagat agcccgccat gtgattaatc aactggcaga
     1981 taaacaaacc attaaacaaa ctttatttgc ccgtcagcca gcaaaaatcc acaaacccta
     2041 tccaactaat agcaagatta ttatgggaga tgtactgaat catgcagcac tgaagcaggc
     2101 catgcaggga caggacgttg tgtatgccaa ccttacgggg gaagatttag atattcaggc
     2161 aaattcagtg atcgctgcga tgaaagcctg tgatgttaaa cgtctgatct ttgttctttc
     2221 cctcggtatt tatgatgaag ttccaggaaa atttggcgaa tggaataatg cagttattgg
     2281 tgagccccta aaaccattcc gcagggctgc ggatgcaatt gaggcttctg gactggaata
     2341 tacaattctt cgccctgcct ggctgactga tgaagatatt attgattatg agcttacctc
     2401 acgcaatgaa ccattcaaag gtacgatcgt atcacgtaag agtgtctcag cattaatcac
     2461 agatatcatc gataaacctg agaaacacat tggtgagaat ataggtatca atcaacccgt
     2521 gactgatggt gacaagccat tattcatgta aatcaattac atagttacca tcgtaatgta
     2581 ctatgtgcag tacgatttat taaacttatt aaggtgaact gatcaatccg atgcgattaa
     2641 catgtgtaga accagtaaga ataaaatacg ccatgtatat aacattacga cttacatcta
     2701 agtcagcata attctaatat ctgcacatca ataaaaatgc aaacaacttc cggcaaaaaa
     2761 gtgatacaag atcgatgaca tagcgttgaa gaaaaggatt tgataatccg caaaatttgc
     2821 acaacactgc ctgtaatact tcctgaacca gagacgatca tgaccgtaaa ccgcccacgc
     2881 gccgaacgcg gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg
     2941 ccgttaatct ggtttccggc cactgcagcc agtcgcgaaa gtggtttgat tctggctggc
     3001 actcacggtg atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gactttgaca
     3061 ccttctttgc gccgccatca tgtagtgctg tgtgtgaatc ctgacggctg ccagctgggg
     3121 ttacgggcca atgctaatgg tgtggattta aaccgaaact ttccggcggc gaactggaag
     3181 gaaggtgaaa cagtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattgctg
     3241 acaggtgaca aacctggctc tgaacctgaa acccaggcgc tgtgccagct tatacaccgc
     3301 attcaacccg cctgggtggt ctcattccac gatccgctgg cctgtattga agatcccaga
     3361 cacagcgaat taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc
     3421 ggttatgaaa cacctggctc tttcggtagt tggtgtgcgg atctcaacct gcattgcatc
     3481 accgcagagt ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg
     3541 gcaaacttgc tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg
     3601 gttccacatc taccgccagc caggtcggtc cgtcaagatc gacgaaactg acctgcggca
     3661 ccagcggtaa agcggcgcta atggcccgag aggtacacaa catgcagccc agcatcagac
     3721 gaaaaccttg tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccggttt
     3781 tatcaagctt aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg
     3841 tatgacaact ttcatctgca caaatcggta acggatgaat aaaattctcc agcgccgcat
     3901 cgtcctgcgc aggaagcggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc
     3961 aacgcgccgc caacccttct gcacgccagg attcatttgc gtcaacgatc agcgtcgcat
     4021 cgggcacagc tgtgcgaatt gccaccatcc gctcactgat aagatggtta tccagcttca
     4081 ctttcagtaa tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag
     4141 gcgtaccgat gactaccgtc tgtgcagtgg tgactgtccc gggaagcgtt atgccgatca
     4201 aatcagccag cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg
     4261 cattacgtgc tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct
     4321 ctaattgcgg cacgacgctc ataatttgcg ccattaccga ggcatcactt tccccataac
     4381 gcggatacgg cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca
     4441 ccacgcgcgc ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg
     4501 cttcctcaaa taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga
     4561 ttaagcgcaa atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta
     4621 actatatgta aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg
     4681 ttacagtcgc caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg
     4741 caaaagatct gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca
     4801 ttttcccgag tattgatacc ggtgtttgcg ccgcatcagt acgtaagttt aaccaactgg
     4861 caactgagat cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt
     4921 ctcgtttctg cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg
     4981 ctgaatttct gcaagccttc ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag
     5041 cgcgtgccgt tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg
     5101 aaatcaccac cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata
     5161 tgcctgatgg tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact
     5221 cagaccatat tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc
     5281 cagtttgcgc gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg
     5341 gctggtgatt tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc
     5401 gtcataatcc ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag
     5461 tgcgcgatag atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag
     5521 tacagtattc aggtcagcgg ccagtttcgg acgcggcacg ccctgctcgt cggcaaagcg
     5581 ggcgacgaac agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag
     5641 attgagcgtc aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac
     5701 cagttcgacc agattcttct gcgtagcgca aatcacccgc acatcgacat gcacctcatg
     5761 gtcttcgcca acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat
     5821 ccgtggtgac atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa
     5881 gaatcctttc ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg
     5941 tatagacgca cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca
     6001 ggcgtaggca aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct
     6061 tagcatcgcc agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac
     6121 aatttgactg aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg
     6181 aatcgttgat cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg
     6241 aagataaaca ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt
     6301 atgggaatct tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg
     6361 tgcggcggta tggttgcgca ggcgatccaa tttttgcgca aaaagctgac agctcgccgg
     6421 gttcgccata tccactttgc ttttcatatc gacagagagc acaggttcgg gcaacgcctc
     6481 cagtaacgcg ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac
     6541 atcggtaaca cctgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc
     6601 cagttcagca aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc
     6661 aatgcctctt agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca
     6721 aaagacttcc agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat
     6781 gacactattt ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa
     6841 cgggcacttc ggcgtaaaga tatccggtca gcacccgata ttgcattatt tttcgctggg
     6901 cgttttgcct ttttgcagcg tttctttcac ctgaccgata agctgacgac ggaaatcccc
     6961 gaggcgaggt ttgtcatcgt caatccacgg cagcgggcgg caaagctcca tagctttaat
     7021 cccgagtcgt gccgtcagaa gtccagcacc aattccctga gctgcgcggg tagacaaacg
     7081 agcagcgaga tcttgcgaca tccagtccat ccccacttcg cgcaccagtt cgctggctcc
     7141 ggcaaaagcg atattcagca ataccagctt aaacagacgc aaacggctgt aataccccag
     7201 ttcaatgcca tacagcgtgg cgatgcgatt aattaaacgc agattgcgcc aggcgataaa
     7261 cgccatatcg accaacgcca gcgggctgac cgcaatcatc aacgttgatt ccgccgccga
     7321 acggctgatt tcgcgccgcg cctgggcatc taaaactggc tggaccaaat gcgcatacaa
     7381 actgaccact tcacggtcgt tttgcgtttc atggattgag gcataccagc gttgcagcgc
     7441 tggatgagac tgatcaatac ccgcctgctg cgccagtttt tcgcaaaatg cgcggccttt
     7501 gcccgtgccg tggctgtgca acaaatcgcg cgcttcgtcg cgttcatggg cgcgctgtcg
     7561 caagcgccat aagcgccgcc actctgttac cacagaacct acgccagcgc cgataatcaa
     7621 tgccccagcg gcacatccac ccagcgccac ccagtcctga gtttgccagg cattcattgt
     7681 ccactgtaca ccctgcccga caacgcttgc gccaaacaga gccagcccgc ccatcaccat
     7741 tttgcgccac aggctgcgtt tcggacgtaa cgctgcatcc attaccgctt caacttgccc
     7801 ctcttcttca ggcgcttcat cgagcgtggc cggggcaaaa ttttgcgcct gattttcgtc
     7861 aaaggtctgc tgcgccctga attttggatt ctgatcgacc tccagcggac cgtcgaaatc
     7921 aatacgtggt tttaacggtt cggtcatcgc aatttatctc ctattaaaaa ttcaagcgcg
     7981 gcatcaagac gaatatgcgg cagcggtttg tcgacatcca tcacctgcgg gcgaaacgct
     8041 tcaaactgga acccttgctt gtcccagaac gcctgaccgg gcaaacgcgc aggaacttca
     8101 ccgggataaa cggtgagtgg cgcaccatcg ctgagccggt taccgcgcaa tgccggaatt
     8161 ttctcgccgt tgacgtcaat aatgccgctg gtggtcgcct gcaccgaggc cagccccagg
     8221 caatccatac taatcccttc aaacgccgca ttttgccagg catcctgaat cagttgttgc
     8281 agtaatgaaa ccatattggc gtgctgatcg atggtcacat ggtccgcttt agtggcagca
     8341 aacaatagct tatcgataac cggcgaaaac aaacgccgga acaaggtacg ctgcccgtag
     8401 tggaaacttt gcatcagctg cgtcagtgcc agacgcatat cattaaatgc ctgtggccca
     8461 ctgttgagag gttgcaggca atcaaccagc acaatctggc ggtcaaagcg cagaaaatga
     8521 ttcttataga accccttcac caccttctcg cagtaataat taaaccgctc gcgcagcatt
     8581 ccggcattgg tgtgcttatc ggcctgcgcc agtttggact cgccccaggc atcgacatcc
     8641 ggccacggga agaattgcag cgcgggcgca cctgccatat ctcccggcaa gacaaagcgc
     8701 ccaggctgaa taaagtgcag cccctgctgt ttacagtggt ggagataatc ggtccacgcg
     8761 gcggcaatgt ctgccagccg gttttcgtcg gcaggcgcta gcgggtccag cccttcgcac
     8821 atcattcgcc atttgaccga ccattcgccg cgctgaccat tgagtaagcc cgtcatctgg
     8881 cgtgaccagc ttaaatagtc ctgcgccagc atcggcaggt cgagcaacca ttcgccggga
     8941 taatccacaa tctccagata cagcgtggag gtgtccttga agtggcgtag cagcgaatcg
     9001 ttcgatttaa aacgtagcgc caggcggatt tcactgacgc cgcgcgttgg cgtcggccag
     9061 gcgggaggat cgccatataa ctgcgccagt ccttcatcat atgtgaagcg cggaatgcca
     9121 aagtcacgct gaggaatgcg ttttacgccc agcaggcgct cttcacgcac cgcgcttaac
     9181 agcggcaagc gtgctccggc gtgaatattg agcaactgat tgaccatcgc agtgataaac
     9241 gctgttttgc cgctgcggct tagtccggtt acggcgaggc gcagatgtct gtcgacaccc
     9301 cgattcacca gcgcattaag ttcattttta agtcgcttca ttgctgtcct gtgctgctct
     9361 gaagaccaga aaaaaatcat tcgtaaggat atgaatgtgg gggccaggcc attgattatc
     9421 aactgcccgt gttgtcatcg acgggcgaac tccataagat gtgcagcatt cacgttcgga
     9481 cggagttcct gacctatgtc ccctactatt tatgatattg ccagggttgc aggcgtatca
     9541 aaatccaccg tatcacgcgt gctgaataag caaactaata tctccccgga agcgcgcgaa
     9601 aaagtgttac gggccattga agaattacag tatcagccaa acaagctggc ccgcgcgctg
     9661 acctcttcgg gttttgatgc cattatggtg atttctaccc gttcgaccaa aactacggcg
     9721 ggtaatccgt ttttctctga agttttgcat gccatcaccg ccaaagctga agaggaaggt
     9781 ttcgacgtga tattgcagac gtcgcacaat ccggcagaag acttacaaaa atgtgaaagc
     9841 aaaattaagc agaaaatgat taaaggcatt attatgctaa gttcgccagc ggatgagtca
     9901 ttttttgccc aacttgataa atacgatatt cctgtcgtgg tgattggcaa agctgaaggt
     9961 caatatgccc atgtttattc tgtcgatacc gataattttg gcgacagcat tgcgttgacc
    10021 gatgcattaa ttgaaagtgg gcatcaaaat attgcctgcc tgcatgctcc gcttgatgtc
    10081 catgtttcag tggatcgggt aaatggttat aagcagagcc tggctgcgca taatattgca
    10141 gtgcgtgatg aatggattgt tgacggcggt tatacccatg aaacagcact gcaagccgcg
    10201 cgggaattat taagccagtc accgttgcct gaggcagtgt ttgctactga cagcctgaaa
    10261 ttaatgagta tttatcgtgc ggcagcagag aaaaatattg ctattccgca gcagttagcg
    10321 gtggtgggtt atagtaatga aacgctgtcg tttattttaa cgcctgcgcc aggcggtatt
    10381 gatatcccaa cacaggagtt aggtcaacaa agctgtgagt tattattccg cttaatttct
    10441 ggaaaaccgt caccacagaa tattaccgtt gccacgcata tgacgctgaa ataaatacca
    10501 gctaccttgc ctgaatgccg gatgcgatgc tagcgcatct tatccggcct acatttatag
    10561 ttcggataaa gtactcgtgc cgcatccggc aatttagccc attatcagaa cgagtaattt
    10621 acgccgacac ctgcataatg gaatttatcg ctgtcgcctt cgtcgtgatc ctgccactca
    10681 aacgcgtatt ccagcgaaac ggaaagcccg ttctggaaat cataaccgta aaataaacct
    10741 acacggttaa aatcatggcc ttcacgttca atatcatcct gccagtccca gttactccag
    10801 cgatccagcc caatgcgcgt atacggcgtc accgagtggt tgccgagcgt cagcggcaaa
    10861 taggcgcgaa tttcttgcgt agaaaactca ccgttattgc ggctgtcgtc catattaaag
    10921 ccgcgctcga gataatagtt cactcgcaag gcaaccgttt cgttgaaggt atattgcagg
    10981 cctgtttccg tttcgactcg ggtatcagcg taaccggtcg tgttcagatc gttggcaaat
    11041 ttatacatcg acaaccaacc gttgaaacgt aaatcgtcag tcagtttcac atcccagtct
    11101 ggcgcgattt tccagcgctg catattcgcc gtgtctttac ccggttcatc aacgtagtga
    11161 taaccataat tacggaaacc gccggtcagg ccgaaactga aatcatcgtt ttcgaggaac
    11221 tgataatgca cctccagctc cgggcgatca aaccacgttc cacgtttacc cgcgctataa
    11281 tctaccggcc cttcctgata ataggccaga gcaattctcc acggcccgtt ggcggcatta
    11341 aaatagactg aaggctccgc cagcccatcc atatcttcgc cataaccctc gacgttttct
    11401 atttcgtaca tcgcgccgat attaaagtgc cagtcgttcc tttcctcggc ctgtgcgcag
    11461 gccattcccg cacacatcac cagtgcggta cagggtaata actttttcat tattgttcct
    11521 tgtattattc tgggggaatg ccttccccct gtatttagcg aattgctatt tccgtttctg
    11581 catcaaagaa atgacatttc gtcatatcaa aatgaatagt gatattttct cctgcatgat
    11641 aatcatttaa cgcaccagca cggaccacta actcgtgccc cccaaccgtg gtgtagagca
    11701 taaattccgc accggttaat tctgccacgc taattttggc ggaaatgtta ttttcctctt
    11761 gcgcgtccgg atgaatatct tccggacgta ttcccatcac gatgggctta tgcaaacttc
    11821 cctgtgtttt cagaaccgct aatttctctt cgggaatggt taatttaagc gtttccgtaa
    11881 cgaatttatc gccatcgatc gtgccacgaa taaaattcat cgccggtgat ccaataaatc
    11941 cggcaacaaa catattcgct ggttggttat aaaccgtttt cggcgcacca acttgctgaa
    12001 caatcccatc cttcataatc acaatccgcg tcgccatggt catcgcttcg gtctgatcgt
    12061 gggtcacgta gatcatggtg gtattcagct tctggtgtag cttgctgatc tctgcgcgca
    12121 tttgcacgcg cagcttggca tcaaggttag agagcggttc atccattaaa aacacgcccg
    12181 cttcgcgcac aatcgcccgc ccgagcgcca cacgctggcg ttgcccgccg gaaagcgcgc
    12241 ccggcttgcg tttcaggtac tcacgcaggc cgagaatttg cgccgcccag ttcacccgct
    12301 catcaatcac ctctttggcg attttttgca tcttcaggcc aaacgccatg ttgtcgtaaa
    12361 ccgtcatatg cggatacaac gcgtagttct ggaacaccat cgctatattg cgtgctttgg
    12421 ctggaacgtc attcattcgt ttgccgtcga tcaacagatc gccgccgctg atctcctcaa
    12481 gcccggcaat catgcgcagg gtggtcgact taccgcagcc cgacgggccg acaaacacga
    12541 tgaactcttt atcggcaatt tccaggttga agtccttcac cacatgcacc tggttatcgt
    12601 agattttttg aatatgttgt aacgaaagct gagccatgtt gattcctttg ctatacgttt
    12661 tgccagaagg ccgataaccg cgaccaggtc agagagtccg ttgaaggcaa cagtaattgc
    12721 gccccggtta agcccgcgcc gatccccacc gagcgcatac cgctggcgtt aatggcgtca
    12781 atgcccgcct gcgcatcttc aatgccgata catgcctgcg gcggcacgcc cagcccggca
    12841 caggcggcga gaaagatttc cgggtccggt ttcgagtttt taagttggga agcatccgcg
    12901 cagaaggtga aaaactcgcg cagctccagc gccgctaaaa tcgtcggcgc attcagggag
    12961 acagaagcca gcccaaccgg gatctgctgt gcacggagat ctgccagcaa agagcgaatg
    13021 ccgggtagaa cagcgctgac cgtcaactcg cgcagtgagt ggacatagag cagatttttg
    13081 cgatacgcca gttgcgccct ctcctgcgag ttaaagtcgc cctctttgcc cccgtgttgc
    13141 agaatgcgcc gcagagactc atcgcggctg atccctttta gggattcgtt aaactgcgca
    13201 tcaatgctga tgccaatttc agcggcaatc tgctgccacg cctggaaatg cagatgcgcg
    13261 gtatcggtga ttacaccatc cagatcgaaa attacccctt gcagtttcat tcatcctcct
    13321 gatgcgtggt agcggtccca ttgaagggca aaataaaatc acccaaacag aaaacagatt
    13381 cttcagccac ggatataagc tgaccgttca aacgcagtga aacgggcgca gaagttcgaa
    13441 tcgcaatacg ctgcgcgtca agagtgacct gtaattcgca gccctgccag aacagaggga
    13501 aagacaactg ttgccactgc tcaggtaacg ccggattgag atgcaattca ccgtcacgca
    13561 cgctcacccc ggcaaaaccc tgaatcgccc ccagccagat agcgccagtt gcggcagcat
    13621 ggataccatc atcacaacta tgcggatcag caccaagatc gatttcagtc ccctcgcgcc
    13681 agaactgata actttgggtc agcaggccac agcgtgcggc aacaatgccg tggattgctt
    13741 tacttaatga cgagtcgtga atagtgcgcg gttcataaaa ttgcagattg gcaagacacg
    13801 atgccgctga gaactgctcc ggcagcatgt aattgagcat caccacatca gcttgtttga
    13861 ggatttgcat ctcgttcact tctgcgcgtg aataatccag cagaatggtt tgcttccccg
    13921 ccgccgcttt gtatttcgcc agattaatcg ccggcttagc cataaacgaa tcatcctgcg
    13981 gcaaaacgcc gtcgggctga atttctggca gccgcagttc tttaaggaac atttcggcgc
    14041 gatggataaa cgcatcgtcg ctacagccaa actggcgggc aatactcagc gcctgctgga
    14101 cgttgtaata cgccatatag ctggtgaagg cgttattatt gacatgttcg gtatattcgt
    14161 caggcccaat aacatcatga atttccagac ggtcgttaac cctcaccgcg cggctaatcc
    14221 agaactttgc agtttccaga agtagcgcca tgccttcatg cgcaatgaaa ctttcatccc
    14281 ccgtggtctg ccagtattga ataaccgccc aggcgatatc ggccaccaga tgatgttccg
    14341 cctgcgccga ggccactttt tgccgcagcc cggtgcgaat gttaatggcg gcaaattccg
    14401 gcgtctcttc ttcgccgctg cgcgcgcttt cccacggaaa tagcgcgccc tgccagccgt
    14461 tgcgtcgcgc tttctcctgc gcgcctggca agttgtgcca gcgataacgc agtaaacttc
    14521 gggcaaccgt cggatcgcta aacagatgaa acggtaacaa aaatacttct gtatcccaga
    14581 aaacgtggcc tttgtagcct tcgccggtta agccttttgc cgcaatgctg ctgcgctcgt
    14641 cgtgggcagg cgtcatgatg cgcagatgat aaagcgcata atctaacgct tgctgatcgt
    14701 gcgcttcgcc gccatttacc gtgatacgac gtttctgcca ccattgacgc cagttttctg
    14761 ttgatactgc aagaagttgg tcgtaactct gctgcgcgca catttcaagc tggcgaagcg
    14821 acgcgctgcc ccactcgtct aaaacggctt gcctgtcatc ccgccagtcg atccacacca
    14881 gtttttgcaa cgtcactgtc tcgcctgcat gaagctgcgc actggtatgt tgctgcaaac
    14941 ggcgctcttt ggcggtatag cattgctgca catcaccgct caccatacag caacagctga
    15001 tggccacatc actgcgtcca tcctgggtgg tgtagatccc ctgcatcaga tgctgaccaa
    15061 acacccgcac ctgggtttcg tcgagatgtt ggcgaccgtg gttggtttgc gtggcgtcga
    15121 tacctgttga aatcagcact gacgcgtcgg cgtccagtgg cgtaatagtg atttccagca
    15181 caatgagcgg cagttggtct gccgaaacaa agcgacggct ggcgatggtg taacctgtgc
    15241 cgttgctggt acgccagaca acgctacggc gtaattctcc actggcaaag tcaagttcac
    15301 gctgccaggc ttcgcgggat aacgagaaaa cctcaccatt tatggcaatc tccatcccca
    15361 agatatcagg caggttcacc agttcgttga tttcaccttt tcccgcccga tgatacagcc
    15421 ccgccagata catccctcgc gtctggcgcg tgtaatcttc ttcatggctg gcgcgaagcc
    15481 caagatagcc gttcccctgc gccatcagcg atgcatactt gttcagggta tgctggctga
    15541 aatggggttc tgataacgtt actggcctgg tcataattcc acacgcgtcc ctgtttctgc
    15601 ggattgatac aacgccgcca ccagttgctg gatgatgtac ccctgctcgg cgtcggctat
    15661 catcacgggc ttgccctgta cgtgattgat aaaggcttcc atgctgcgca aatggcggtt
    15721 gtcgtctgcc agttcccgtt gcatcagcgt cattaattca ccgttgttat cggtgtagat
    15781 atgtgctgga aacagcgtcg caccggcttt atcaccacaa aagctgacgt tcataatcga
    15841 ctgttcgcgg atgttgagtg caaatgacgt ttccagccac agaatgccgc cgttatgaaa
    15901 ttcaatggtg ccaaataacg aatcttcgac gctgtaagtt gccggatccc actcgccaaa
    15961 ttgaccgcag ctcttttgcg tgccgatctt ttgaaagcta tgcgcattca cgcttttcac
    16021 cgccggaaag cccagcacat acatcgccgc atccagcata tgaatgccga tgtcgataag
    16081 cgggccacca ccctgcagtt ctttattggt aaagacaccc cagccgggaa cgccgcagcg
    16141 acgcagggcg cgggcggtgg taacgtaaat ctctcccaaa acgccgttgg tcacctgttc
    16201 acgcagctgt tgcgtatcga gcgcaaaacg gtggtgaaag tcgtaggcca gcaccttgcc
    16261 cagtttgcgc gcggtatcgc acatttcccg cgcctgttct ggcgtcatgg cgggcggttt
    16321 ttcgcacatc acatggcagc ccgcttccag tgccatcagg gtatgttcgt aatgaaaacg
    16381 gttaggtgag cagacgctaa ccacatcagg tttcaccgcc agcagcatgg cctgcggatc
    16441 gtcccacacg gatgcattac cgtatttatc tgccagcgcc tgcgcctggg aaaggcggct
    16501 gtcacagaca gccaccagtt ccagatcgtt gcgggtgcag tagtacgaag catgaacctt
    16561 atccgccacc tggcctgcgc ctattatcgc gacccgcagc ggagagcttg tcattgcact
    16621 tttcacaaag acctcttagc aggtacgcaa ccaggccaac gaatcacggt acgcctgggc
    16681 aggctcttcc gcccggatac gcccttcgta cactacatag ccctgatagt tatccgcgcg
    16741 cagctgttca aacagcgcgt ggaaatccag ggtgccgctg cccggctggt agcgatggtt
    16801 atccgcaata tgcacatgac cgagcaggtc gcggttgtca tgcagcgcct gcgccaggtt
    16861 atcttcttcg atattcatgt gatagaaatc gccgataatc tgtacatgtt taagatcgtt
    16921 ttcgacgatg taacggcggg catcggcgag ggtgttgatc atatgatcct gatagcggtt
    16981 taacggttcg agatacacca cggttccggt acgcgcggcg acctgttcca gtacgtgcag
    17041 ggaatcactc accattttgc ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa
    17101 gcggaaggta aacatgcccc acgctgccgg aacgacgata cctttacccc ccacttctgc
    17161 cagcgcttcg agaatgcgtt cgatctgctt taagccatta agacgacgct cttcgataaa
    17221 gtcgccaatc caaccgtcat agccaccgca ggcggtggtc accggtaaac cggtttcttt
    17281 gattgctgct ttgacttctt cgagattgtt gaccagcagt ttgccgtcaa tctcaaaacc
    17341 atcgaagccc atctctttga tataacgaaa tttctccaga atgttttccg gaaagaatgc
    17401 ctgattctgt gtgcctattt tcattttttc attcctttga attaaaacgt aacgcccatt
    17461 ttgatgctct gttccggatg ctgatcgaca tactgcatgt agctttccgg gctcttggca
    17521 aaggtcacta ccgggtcgat taaatcttcg caattgagat aaccgttcat cagcagttcc
    17581 cagcaggttt cttcaatacg cttacggctc cagcgcggat aatccgggtt cggttcgctg
    17641 cacgcacggg agaaaacaat tttggcgtta ttgaaatgcg cttcgcgtcc gaggttaaaa
    17701 ccttcagcaa atggtttggc aaacgcgaca taagagatgg tgccgccata agcaaggcca
    17761 cgcagcgccg attgcagcgc gtcggcgtaa ccgctggttt cgataatcac atcggcaccc
    17821 tgcttgccgg tcagcgtttt gatctctttt cctacgtcag tgccaatgag gttaaggcag
    17881 aaatcagcac cgtggcgacg ggcaatatca cagcgatagg cgataggatc gacgccaatc
    17941 accaccgacg cgccagcgcg tttcgccagt tggatggcta tttgaccaat cgcgccaagc
    18001 cctaccacca ccacaaaatc acccacgcgc acgttggcat cgcgcacgcc gctcatggca
    18061 aactgcgccg ggtcgtaaca aacggcgttt ttccacgagc tgccttccgg cattttgcgc
    18121 agcttgtagt tattcaccgc gttaatgatg accgtctcgg agaacgggcc gtagccgcat
    18181 acgctgtcgc ccaccgcgta gtcggtaacg tcgctgccgc actcgataat gtcgccaacc
    18241 accatgttgc caagctggaa tttgccaaac tcaatgccgc gcggcgcatc tgccggacgc
    18301 ggagtgaaca tctgccattc gccgttaaag tcttcatcga taaacgggct ggcggcgcgg
    18361 aagtcgacca cttccgttcc gtgtttcggt gcgccgaaac gcacgcggat tttcacttca
    18421 ttggctaaaa tcgcccgatc ttcatactca accagcgcag caacacgcgg tgctgtggct
    18481 actaactttt tcatttaaaa ctccttgtaa agaaactggc cgctttagcc cttcacgcca
    18541 ccggcggtca aaccactctt aataaaacgt tccgaaagcg catacataat caccaccggc
    18601 aatgcggtca ccagtgaggc cgccatcatc cgtccccaga tgtaatctgg cgtactgaac
    18661 agcgcgttca ggcccaccgg taaggtgaaa ttgctggcgc tggagaggaa aatcgacgca
    18721 aacagatagt cgttccacgc caccataaag cagtagacga acacggatat cagcccggac
    18781 atcgccaacg gcacggtgat gcggaagata atttgcagac ggttgaggcc atccatcatc
    18841 gccgcttctt cgatctcatc ggggatggtg tcgaagtagc ttttcagcat aaacacggcg
    18901 gtgggcagag tttgcgtcac catggtgatg atcagcgcca tctcggtgtc gtaaatgccc
    18961 agcgcggtga tgattttgaa cagcggcacc accagcaaaa tgccagagaa catatacacc
    19021 gtgtaaaagc tggcattgat ggtcatccga cctttaaagc gcaggcggga aagcgcgtaa
    19081 gccccaagaa tgccgaggaa taccgccacc acggatgaaa ccaccgacac caccagactg
    19141 ttacggaagt agtcgacaaa cgggaaaatc atcgggttaa aaatgtcgac gtaatgctcc
    19201 agcgtccact gctgcggcag cagcgtagga tgcagtgaga tcgcctcttt cgcgcccttg
    19261 aacgaggtca tcagcatcac aaaaaatgga aacagcgtga tgatgagaaa cagcgccagc
    19321 ccgcagtaaa aaccgatgcg actgagtgtg cgtttatttg ttgccattga ggttcacccg
    19381 tttcctggtc agcagaatga cagcgaaaat aatgatgaag agcaccaccg agatcgccgc
    19441 cgcttttcct aaatcattaa aggcaaaggc ggttttgtag agatatacac cgagaatatc
    19501 gaccttggtc gtcagcaaat aaacatccgc gaacatgtag aacatccaga tggtgcgcag
    19561 ggtcaccacc gtcgccagga ccggcataat cgcgggcagc gtgacgatac gaaaccgttg
    19621 ccaggcatta gcgccatcca tctctgccgc ttcgtacagc gatttgtcga tggtctgcaa
    19681 aatcgccaga aacgagataa aggcatacgg gaagtagcgc cagatggcga acagcaccac
    19741 cagcacaaag ctactgcccg gattatcgaa ccacagcggt gcctgctcat agagatgcag
    19801 aagatcgacg ccgaggtagt tcacaatgcc gtagccgttg ttgaacatgt atttccaggc
    19861 aaacaccagc gaaatggacg gcgttacgta ggagaggatc accagcgaac gcgcggtttt
    19921 gcgcagacgg aactcgcggt tgaaaaacat cgccactgcc agcccgagca cggtgctgcc
    19981 cgccaccacc agcgcggtat accagaccgt catccacagc gagtgccaga agccgggatc
    20041 ggagaggata cgcacatagt tactcacccc aacaaaggtt gactcgatgt tgggattgag
    20101 cggcagacgt aaaaaactga tttcgatatt cgacaccatt ggccaggcca ccagaccacc
    20161 cagcagtaat aagctgggcg cgagaagcag cagcgcaaag ggcatatcgg aacgacctga
    20221 aaaaagcctg ttcattgcgg attcctgtcc tgggtttagt gctgttcgat cagttcatcc
    20281 agctttttct ggctcgcttg cagcgtgcca gggagatcgg ctttacccac ggtgacgtta
    20341 tgcaccatgg agctcaccac gccagacccc gtcacatcac ccatgcgggt aaagttttta
    20401 tcccctactg caccaaagac ctgaatattt ggcagttcac cgattagctg attcggtagt
    20461 tcacccagcg ccttaataac gtcgttgtct ttccaggtgg cggtagtgac aaccgcttta
    20521 ttcaccggca gcgcagcacc tggcgacatc atcacccagt cggcaatgtt gtctgcctgc
    20581 tccataaagg tgacaaattt ttctgctgct tccgtctctt cggtcttttg cccggcggta
    20641 atggtcagcg aggtcaacat gccgtagacc gcagagtttt tctcggttgg caccacgaaa
    20701 ccgacgtttt tcgggtcgcc ttctttaatc acagccggaa ggatataggt ggagtaaatc
    20761 gccatcggcg cggtgccgtt cataaaggcg tctttcactt ccatgatgtc gttagaaccc
    20821 ggcatagtgt tggcagcaag gtcgcggtaa taggtcagtg cctgcatcat ctctggtgta
    20881 tcaagggtga ttttgccttc ggcgttaaag acgttagcct ggttggataa cgcaaactgg
    20941 gagaaggatt gttccgtcaa cacgctttct gctgtaggca gcgcaatgcc gtatttttta
    21001 ttcgccgggt cattcagttt ctgtgcaacg tccagcagct gttgccagtt tttcggctcc
    21061 tcaagccccg cttttgccag cacatctttg cgataccaga taccgccaat ccaggcgctg
    21121 acaggaacac cggtccatgc gctaccatct tcggtacgca caatacgcag tacgccatcg
    21181 taaaacgcgc cttcaccaac gttgctgatg actgtggcaa ccgctttgcg atcgataagc
    21241 tgctctttgt ccatcacttt ggcgtagtca tggctggttt ctatcacttc cggcagcgaa
    21301 ccgctacgtg aaagagtaat gactttagtg ttataggcat cttcttccac gggcacttgc
    21361 ttaacggtga tgccagggtt ttctttttca aaacgggcaa tcaatttact gataacggcc
    21421 tggcgctcct gctccaccga agaatgcata aattcgatgg atacattcgt tttattttct
    21481 tctttacacc ctgaaatcag ggcgcaggaa accagtgctg ataacagcac gatttttgat
    21541 ttaatcatga gatgttcctt tttatttaat ccacataacc tgccaggggc gcagcgttat
    21601 ttcattaccc aatatatcaa cttcactaat cagatcccga ccatgcagat tagtaatatt
    21661 tacatgctga atatttttac tgacattaaa caatccagtc agacaattac catcagcgtt
    21721 acttctttga atacgcatta cggatgaatt aatcgtatcg atggtaaaat tattatccgg
    21781 atgaaactcg ttatggctgc gacgaagtgt aattaaacgc gacaactcat gatataccgc
    21841 atgccttaat gtagcttcat cgttcagttc tcgggttatc tctttactgt gatatttttt
    21901 acggttaatc gcacggttat atccgagttt ttcgacacct gcgtaatcat tacgcgagcc
    21961 aagaatactt tgaatatata tcgctggcac accggggaaa cttaacaaaa tcgcatgggc
    22021 gagaataaac ctggcgcaac gttcttcatc gctactctca cggcggctta acgcatccat
    22081 ataggtcaca tttatttcat acggactgcg tgtaccgtcg ggattatttt tccagtttac
    22141 taatgcacct tcctgctgta acgcctcgac cagctctaat atttcgcttt caggcaacaa
    22201 gccccgtagc gggtttagcc cgatgccatc gtgagaggcg aggaagttaa accaggtggt
    22261 gttgctggaa ggtagtgtca ggttttgcgc ccacgcacaa agcgcctcaa cgttctgttt
    22321 ttgcaccgca tgcagcacca gtggcggcag cgagaactgg tacaccatat gtgcttcgtc
    22381 atcgcctgcg ccaaagtaag caatgttgtc tttatgcgga acattggtct cggtaatgat
    22441 cactgtacct ggcgcaacgt tatcaataat cgaccgtaac agtttgataa tcagatgtgt
    22501 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
    22561 gcggacatac tcagcacctt tcgcaaggta acagagtaaa acatccacca tcgccagcaa
    22621 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
    22681 gcgcgttgaa tgatcgcgca tctggaatgg cgttaataac ggtaacgcac gcgggcgagt
    22741 gacggcgctg agatcggttt gcgggtcaac ggcaataaaa aaatcttcaa aacctggatg
    22801 ctgttgtaaa tagtttttaa atcattcact ttttgccgac atatggttgc agacaaaatc
    22861 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
    22921 ggcgacctga tgataatcaa ttaccgaaaa gccatcatca gatgaccatg gataaaacgg
    22981 caacaaatga acatgtgaaa aaatgctttg cagccattga tggtaaaact gattaaatgt
    23041 gggtaatggt tttaaatcat tgctgtgaaa ttgatcggca taggtaatta acacgacatc
    23101 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
    23161 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccaggta
    23221 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga aacgagagac
    23281 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
    23341 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatgg ggatgtaaat
    23401 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
    23461 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
    23521 tctcgctaag gatctctttt gcttgccctg actggcgtcc ggcattgcaa tacactttca
    23581 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
    23641 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
    23701 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
    23761 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
    23821 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
    23881 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
    23941 ttttatcgcc cagcccgcta cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
    24001 cttgcctgct aatttgaaac caggctttac cttttgcccg gcctgttgcc agcgagtatt
    24061 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
    24121 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
    24181 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
    24241 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaagaaaatc
    24301 gacagcacca ccaggatacg caccagtttt gccggtacat caaaatagtt ggcaatcccg
    24361 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt attgagatta
    24421 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
    24481 cctgaatacg ttcgcgcatc cgttttgctt catcagccag ttgcgctaat cgctgctgct
    24541 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
    24601 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
    24661 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
    24721 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
    24781 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
    24841 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
    24901 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
    24961 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcac cgatctcttt
    25021 cttcatgcgt gccagcgtgt cgtccaccag cgtcacttca tgttccaggc tcttaatcag
    25081 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
    25141 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
    25201 gcgagtcagc tgtttctttt ctgccaacgc gcgcgccgaa gtagaacgta cttcaaccag
    25261 tgtatcttcc atctcctgga tcatcagacg aaccagtttc tgtggatctt ccgctttctc
    25321 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
    25381 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
    25441 gccaattttt tatctttttg atttataaag atctgattga agaaccaaca gcaacatgcc
    25501 aggatgagtt agcgaattac actaacaagt ggcgaatttc atcatggcag aatacaaaga
    25561 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
    25621 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agctgattgc
    25681 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
    25741 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
    25801 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
    25861 tgatgaactc gctacggcac cgatgatggt gcaggagaaa ttattgcgcg tgattgagta
    25921 cggtgaactg gagcgcgttg gcggcagcca gccattgcag gtgaatgtgc ggttggtatg
    25981 cgcgacgaat gccgatctcc cggcgatggt caatgaaggc acttttcgcg ctgacctgct
    26041 cgaccgactg gcttttgatg ttgtacaact gccaccactg cgcgagcgcg aaagcgacat
    26101 aatgttgatg gcagaacact ttgccatcca gatgtgtcgg gaaattaagc tgcctctgtt
    26161 cccggggttt acagagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
    26221 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
    26281 gcttgatgac atcattattg atccctttaa acggcgtccg cctgaagacg ctatcgccgt
    26341 ttcagaaacc acctcgcttc caacactgcc gctggattta cgtgagtttc agatgcagca
    26401 ggaaaaagag ttgctgcaac tcagtttgca acaagggaaa tataaccaga agcgtgcggc
    26461 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
    26521 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
    26581 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
    26641 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccggtat
    26701 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
    26761 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
    26821 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
    26881 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
    26941 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
    27001 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
    27061 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
    27121 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
    27181 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
    27241 ggcaaccagc tcttttggcg caacgttgaa accgccctcg ccctgcaccg gttcgaaaat
    27301 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
    27361 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
    27421 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
    27481 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
    27541 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcaccg gtagtgaaga atgcggtttt
    27601 agcctgcccg ctcactgggg caagggcgtt aattttctcc gccagcgtga cgtagctttc
    27661 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
    27721 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
    27781 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
    27841 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
    27901 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
    27961 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
    28021 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgttcggcg tcgccgcgca
    28081 gcagttcggc aatcagacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtagc
    28141 cctgcatgta atagatgttg gtatcaaggc gaccgaactg cggcatgcgc gacaaggtca
    28201 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcagctgc gggaaggttt
    28261 tcagcagttt cggcaccaca aggcgctcaa cgtcatccgg gtcgcgcgcg ccgtagacca
    28321 cgccgccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
    28381 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
    28441 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgccagt tccggctcta
    28501 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgacctt
    28561 tggcagttcg cacaacggct ggcgtggtgt gctggatctg cgtcacggca gaaagttcat
    28621 acacgcgccc gccgttgagg cggatggcgt ccgcttcgcc aatggcgagg tttagcggat
    28681 gaatatgccc accgctgtga tccagcagcg caccggtata gcgatcgctg gctacttcac
    28741 ggcgaatggc gttggcgtcc agcaattcca gctgtttatt gccgtagcgt tcccagttct
    28801 ctttctgctc ttcaagtgtg gcgagctgtt tatcattcat cgccacaaac aggccgccgg
    28861 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcaccacctt
    28921 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
    28981 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
    29041 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
    29101 aagagagccc ggtatagccg ccgccgacca cgcaaacgtc gcaggaaatc gactcattca
    29161 gcgtgtcgaa tggcgcgtat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
    29221 tcatttcagg cctccaggct tatccagatg gttttcagtt cggtgaattt ttcaagggca
    29281 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
    29341 tcgccgtcgt tgtagttatt gacgaagacg gaaccagcct tcaggcggcg gctcatgcgg
    29401 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
    29461 gcaagctgta gcgcctggtc ttctgatgtg aaacgcgtga ccaccagcac cggaccgaaa
    29521 atctcttcgc ggcttaagga cgcattcggg tctacatcca caaagatggt cgggccgatg
    29581 gcggcagcca gctcagcgtt acgaccatcc agcagcagtt gccctttgct ttcgccttcc
    29641 cgaataaagc tatggaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
    29701 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
    29761 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgca cacctggccc
    29821 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
    29881 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacgcgttt catgttgctc
    29941 tcgcccgcat ctttcagcag ctgtttcccg gtacgggtcg aaccggtaaa ggcaatggcg
    30001 tcgatatcgt tatgacgcga cagcgcctgc cccgcttcat gaccaaaacc tgtcaccacg
    30061 ttcaaaacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
    30121 agcggtgatt tttcagacgg tttcaggaca acgctgttcc ctgctgccag cgccgggcca
    30181 agcttccagc aggtcagcag cagcggaaag ttccacggta cgatggcggc aatcacaccg
    30241 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
    30301 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
    30361 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
    30421 gcgtgggctt ccattaaatc ggtgagttta ttcagtaccg ctttacgttt agccggagaa
    30481 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
    30541 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
    30601 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcgggtttca
    30661 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
    30721 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
    30781 gccgtatgcc cgtttatttc tggcgacatt ctttatatac acttcatcct tcaagccgcc
    30841 tctgccttgg ctgcgttcgc tcccccgaat cacttacctg agtaaactca tcgggattcg
    30901 ctcacttgcc gccttgatgc aacttgaatg attttgtgta tagattgccg ggcgcattgc
    30961 gcttacccgg cttacagaac aaaaagatta aaacgtggtg ggcgtatggg cgctgataat
    31021 tcggcaaata cctgccgacg tattactgaa actgtgcggg atgccggtat taatggcata
    31081 actttgcccc gcgacgaggt ggtaatcctg accattaatc gtcagaacaa tttcaccttc
    31141 cagtactgtg cctatttcct caccctgatg cttaattctt tccccagtgg ttgtgcccgg
    31201 ctggtacgtt tcaaagatca tcgccagcgt gcgattcggg ttaccgttat gaaccagctt
    31261 cattgacaca ccctgactgc ccatctcaat taagtcgtcc tgattaatga cgacctgcgg
    31321 ctcatcaggt ttttccggct cggaaaagaa ttccgagagt gacagaccat acaccttcag
    31381 cagcttttgc agcgtactga tggcagggct gactttatct tgttctatcg tactgatagc
    31441 actgtgagtc agcccggaga gttcggcggc acgacgttgt gaaagcccct gctgctggcg
    31501 gatttccgac aagcgttttc ctggcgccag tccctcatca ctcatatttg catttcctta
    31561 aactgtagtg gtcagagtcg ctgtttttca gcgatatggt gctgacaagc ggtgataaag
    31621 ccctcgaaca atatacgcga aagcgcgtac tcgctactgt tccattccgg gtgccactgt
    31681 acgcccagcg caaaaggatg attgatgacg ctaaccgcct caactaagcc atccggcgag
    31741 cgagcttcaa cacgcaaccg tgggctaacg accttcgccc cttgcccatg cagagagttt
    31801 acccaaaagt tgctacattc aggtaacaac gcagacagta atcccccctc ttcaacctga
    31861 acttcatgcg acggtgcata ttgctgttcc accggcagtt cgggatcttc ccgatgttcc
    31921 agcaattcag gctgttcgca cagcttgcga tgcaacgaac caccggttgc caccaccagt
    31981 tcctgtaaac cccggcagat ggcgaaaatg gggatgcgcc tttcgagtgc ggcattaatt
    32041 aacgccatgc tcagaagatc acgcccggga tcggcgtcag gctcatcgcc gttttcacca
    32101 tataggtgcg gctgcacatt gctgggacta ccaggaagat aaatgccatc gagtttcggc
    32161 aaaagttgtt caagtaatga cggttccgcc agcgcatgtg gtagcgcaat tggcaagccg
    32221 cctgcgtgga tgatggcatt caggtacttt tcttgcagag tctgggtcgc atgaccctta
    32281 agcctgttcc tgcacattac gacaccgata accggattgt tcattatatt ttccatgctc
    32341 aatctcacaa agtggactaa attatcgcca ttactgcggt ttaaatgacc tttatgttca
    32401 atattttttc aatctagcag tgggttttca tttttgcaaa ctcaatttaa catttgacaa
    32461 acatttagtt tgcatacaga ttcgaatggt ggtcattata ttttacgctt tgataacgag
    32521 cggaaaacaa accaaaggcg aagaatcatg gaaaccaata tcgttgaagt agagaacttt
    32581 gttcagcagt cagaagagag gcggggtagc gcctttacgc aggaagtgaa acgctacctg
    32641 gagcgctacc cgaatacgca atatgttgat gttttactta ctgatttgaa tggttgcttc
    32701 cgtggaaaac gtatccctgt ttcaagcctg aagaagttgg agaaagggtg ttatttcccg
    32761 gcctcggtgt ttgcaatgga tattctgggc aacgtagtag aagaagcggg tctgggtcag
    32821 gaaatgggcg agccggatcg tacttgtgtt cctgttctcg gttccttaac cccttctgcc
    32881 gccgatccag agtttatcgg tcagatgctc ctgaccatgg tcgatgaaga tggcgctccc
    32941 tttgacgttg agccgcggaa cgttcttaac cgcctgtggc agcagctacg ccagcgcgga
    33001 ttgttcccgg ttgtagcggt agagctggag ttctatttac tggatcgtca gcgcgacgct
    33061 gaagggtatc tgcaaccgcc ctgcgcgccc ggcaccgatg accgcaatac acaaagccag
    33121 gtttactccg ttgataacct caaccacttc gccgatgtgc tcaatgatat tgatgaactg
    33181 gcgcagttac agttaattcc cgccgacggt gcggtcgccg aagcctcgcc gggtcagttt
    33241 gaaatcaacc tttaccatac cgataacgtg ctggaagcct gcgatgatgc gctggcgcta
    33301 aaacgcctgg tgcgtctgat ggcagaaaag cataagatgc acgccacttt tatggcgaag
    33361 ccgtatgaag agcacgcggg cagcggaatg catatccata tcagtatgca aaataaccgt
    33421 ggcgagaacg tgctttctga cgcggaaggc gaagattcgc cgctgctgaa aaagatgctc
    33481 gccgggatga ttgacctgat gccgtcgtcg atggcgttgc tggcaccaaa cgtgaactcg
    33541 tatcgccgct tccagccggg aatgtatgtg ccgacgcagg cgtcgtgggg ccataacaac
    33601 cgcaccgtcg ccctgcgtat tccgtgcggc gaccgtcata atcaccgcgt ggaatatcgc
    33661 gtggcgggtg ccgatgccaa cccatatctg gtgatggcag cgatttttgc cggtattttg
    33721 catggccttg ataacgagct gccgttgcag gaagaagtcg aaggcaacgg gctggaacag
    33781 gaaggcttac ccttcccgat tcgccagagc gatgccctgg gtgagtttat cgagaatgat
    33841 cacctgcgcc gctatttagg cgaacggttt tgccatgtgt atcacgcctg taaaaatgat
    33901 gagctgttgc agtttgagcg cctcatcaca gaaacagaaa ttgagtggat gttgaaaaac
    33961 gcgtaattcc ccatgcgggg ttgtttcatc ggcctgagcg gagccaggcc tgacatttgc
    34021 tggtaatcca gacatttccc ggatgtcgcg ttatggagcg caggcggcaa cgggcttccg
    34081 ttaacgatta agactgtgac gaggaaatga gatgatgccg atgattaaat ccccgcatgg
    34141 cgagggaggc tgcgtatgcg cccctcctgc tactgactgg actccgcccc ctttgcttcc
    34201 cttgctaaac aggtttgatt tcaggtcgac acgaccgcaa accttattac gcaggggagg
    34261 cagcaattat ggctattaat tcaccactga atattgctgc gcaacccggc aaaacccgtc
    34321 tgcgaaaatc actgaaattg tggcaggtgg taatgatggg tctggcctat ctcacgccga
    34381 tgaccgtatt tgatactttt ggtattgtct ccggcattag cgacggtcac gttccagcgt
    34441 cctatttgct ggcgctggcg ggcgtgctgt ttaccgctat cagctacggc aaactggttc
    34501 gccagtttcc ggaggccggt tcggcctata cctacgcgca aaagtcgatt aatccgcacg
    34561 tcggatttat ggtcggctgg tcatcactgc tggattatct ctttttgcca atgatcaacg
    34621 ttttgttggc gaaaatctat ctctccgccc tcttcccgga agtgccgccg tgggtgtggg
    34681 tggtaacctt cgtcgccatt ttaaccgccg cgaatctgaa gagcgtcaac ctggtcgcta
    34741 acttcaatac cctgtttgta ctggtgcaaa tctccatcat ggtggtgttt atcttcctgg
    34801 tggttcaggg actgcataaa ggagaaggcg ttggcaccgt ctggtcactt cagccgttta
    34861 tcagcgagaa cgcgcacctg atcccgatta ttaccggtgc gacgattgtc tgtttctcgt
    34921 tcctcggttt cgatgcggtg accacgcttt cggaagagac gccggacgcc gcacgcgtga
    34981 tcccgaaagc catcttcctg acggcggtat acggcggcgt tatctttatc gcggcgtcgt
    35041 tctttatgca gctgttcttt cccgatatca gccgctttaa agacccggac gccgcactgc
    35101 ctgaaattgc gctgttcgtc ggcggcaagc tctttcagtc gattttcctc tgcaccacgt
    35161 ttgtgaacac attagcgtct ggcctggctt cacatgccag cgtgtcgcgt ctgctgtatg
    35221 tgatggggcg tgacaatgtg tttccggagc gcgtgtttgg ctatgtgcac ccaaaatggc
    35281 ggactccggc actcaacgtc attatggtcg ggattgtcgc gctgtcggcg ctgttctttg
    35341 atttagtcac cgcgacagca ctgattaact tcggtgctct ggtggcgttt accttcgtga
    35401 atctgtcggt gtttaatcat ttctggcggc gtaaaggaat gaataaaagc tggaaggatc
    35461 acttccacta tttgctgatg ccgctggttg gcgcgctgac ggtgggtgtg ctgtgggtta
    35521 acctagagtc aacgtcactg acactcggtc tggtatgggc ttcgttaggc ggcgcatatt
    35581 tgtggtatct gatccgccgc tatcgcaaag tgccgctgta cgaaggtgac agaacgccgg
    35641 tgagcgaaac gtaatagccg gatgcgcggc gcattatcct gcctgagggt aacgcgccgc
    35701 gccattaact ataaaaagat gtatgacccg gatcacgagt gctatcatcc gcccttatgc
    35761 ttagcgcatt aaccccaggg gcaaataatg aaccacgata tcccgctaaa atattttgat
    35821 attgccgatg agtacgcgac cgaatgcgca gagcctgtcg cagaggctga acgcacaccg
    35881 ctggcccact acttccagtt actgctcacc cgtttgatga acaatgaaga gatcagcgaa
    35941 gaagcccaac atgaaatggc tgctgaagcg gggattaatc ccgtacgcat tgatgagatc
    36001 gcagagtttt tgaatcaatg gggtaatgag taattattta attgcagacg atcggtcagt
    36061 atcgttgtcc ggataaagcg ttcacgccgc atccggctaa caatgacacg cactgcagcg
    36121 tgctttaatg ggtttctcac gatcgtaccg ctctgctcct cgctaagttg ttctgtgagt
    36181 tatagttata gagaaatcac gggtacgaat gttgaacaga tggcgacgcc acctgttcaa
    36241 cgaccaacca cacggccctc attacgaccc ctgaacacaa acggtaatgg caaatgtcat
    36301 taaatattag taagatagcc tctttacata aaatccctta atattatgcg ccaggtatta
    36361 tcgtctcttt tggtgattgc tggacttgtg agtggtcagg caatcgccgc gcctgaatcc
    36421 cccccgcatg ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg gcaagtcaac
    36481 acctttaacc catccaaagc gagcagtggg ttaattgtcg atacccttgc cgcccagttt
    36541 tatgatcgac tgctggatgt cgatccctat acctatcgcc tgatgccgga acttgccgaa
    36601 agctgggaag tactcgacaa cggcgcgacc tatcgcttcc acctgcgtcg cgatgttccg
    36661 tttcaaaaaa ccgactggtt tactcccact cgtaaaatga atgccgacga tgtggtgttt
    36721 accttccagc gaatttttga ccgcaacaac ccgtggcata acgtcaacgg cagcaacttc
    36781 ccctacttcg acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa actggataat
    36841 cataccgttg agttccgact cgcccagccg gatgcttctt ttttgtggca cctcgcaacc
    36901 cattatgctt cggtcatgtc ggcagaatat gcccggaagt tagagaaaga agatcgccag
    36961 gagcaactcg accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga ataccgcgcc
    37021 gggcaattta ttcgcctaca acgtcatgat gacttctggc gcggtaaacc gttaatgccg
    37081 caggtagtgg tggatttagg ctccggcggc accggacgtc tgtcgaaact cctgaccggg
    37141 gaatgcgacg ttctggcctg gcctgctgcc agccagctat ccattttgcg tgacgacccg
    37201 cgcttgcgtt taacgctgcg tcctgggatg aacgtcgcct atctggcatt taacaccgcc
    37261 aaaccgccgc taaataatcc cgctgtccgc catgcactgg cactggcgat taataaccag
    37321 cgcctgatgc aatccatcta ttatggtacg gctgaaacgg cggcctctat tttaccgcgc
    37381 gcctcgtggg cctatgacaa cgaggctaaa attactgaat acaatccggc gaaatcgcgc
    37441 gaacagttga aggcgttggg gctggaaaac ttaacgctga aactgtgggt gcccacacgt
    37501 tcgcaggcgt ggaaccccag tccactgaaa actgccgaac tgattcaggc ggatatggcg
    37561 caggttggcg taaaagtggt gattgtgccg gtagaaggtc gctttcagga ggcgcggttg
    37621 atggatatga gccatgatct gacgttatcc ggttgggcga cggacagtaa cgacccggac
    37681 agtttcttcc gtcctttact gagctgcgcg gcaattcatt cacagaccaa cctcgcccac
    37741 tggtgcgatc cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca gcagctggcg
    37801 gcgcgtattg aagcctatga cgaagcgcag agtattctgg cgcaggaatt gcccattttg
    37861 ccgctggcgt cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg tctggtactt
    37921 agcccgtttg gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga tgaggtgaaa
    37981 aaaccatgat tatcttcacc ttacgccgca ttttgttatt gattgtcacc ttgttcctgc
    38041 tgaccttcgt tggcttcagc ttgagctatt tcacccctca tgcgccgtta caaggcgcgt
    38101 cactgtggaa tgcctgggtg ttctggttta acggcctgat ccactgggat tttggtgttt
    38161 ccagcattaa cggtcaaccg attgccgaac agttaaaaga ggtcttcccg gcgacgatgg
    38221 aactgtgcat cctcgccttc ggttttgcgc tgattgttgg catccccgtg gggatgattg
    38281 ccgggattac gcgccataag tggcaggata acttgatcaa cgccatcgcc ttactgggct
    38341 tttcaatccc ggtgttctgg ctggcgcttc tgttgacgct gttttgttca ctcacgctag
    38401 gttggttgcc cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg attaccggtt
    38461 ttgcgttgat tgatgcctgg ctttcggact caccatggcg ggatgaaatg atcatgagcg
    38521 caatccgcca tatgatattg cccgtgatca ccctgtcggt tgcgccaaca actgaagtga
    38581 tccgcctgat gcgtatcagc actatcgaag tgtatgacca gaactatgta aaagcggcgg
    38641 cgacccgcgg tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat aacgcgttac
    38701 cgccggttat tcctcgcctg ggcttacagt tttccaccat gctgacgctg gcgatgatca
    38761 ccgaaatggt ttttagctgg ccgggtttag ggcgctggtt aattaacgcc atccgccagc
    38821 aggattacgc agccatttcc gccggagtga tggtgtgtgg ctcactggtc attattgtta
    38881 acgtgatttc tgatattttg ggtgccatgg ctaaccctct gaaacataag gaatggtatg
    38941 ccttacgata gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac cgcctggcgc
    39001 aaattttata gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg gctggctgta
    39061 ctgtgtattt ttggcggctg gtttgcgcct tacggcatcg accagcaatt tctcggttat
    39121 caattattgc cgccgtcatg gtcgcgctat ggcgaagttt ctttcttcct ggggactgac
    39181 gacctcggac gcgatgtttt aagccgttta ctgagcggag ctgcgccaac cgtgggtggc
    39241 gcatttgtgg tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac cttcgccggg
    39301 gcgacacacg gcctccgctc agcggtgctt aaccatattc tggatacctt actggcgatc
    39361 ccttcgctgc tgttggcaat tatcgttgta gcgtttgccg gaccgagttt gtcccacgct
    39421 atgtttgctg tctggctggc attgctgccg cgtatggtac gttcgattta cagcatggtg
    39481 catgacgaac tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc atcaacgctg
    39541 aatattctct ggtttgctgt gatgccaaac atcaccgccg ggctggtcac cgagatcacc
    39601 cgcgcactgt cgatggcaat tctcgatatc gccgcgctgg gctttctcga tctcggcgca
    39661 caactcccct cgcctgaatg gggagcgatg ctcggtgatg cgctggaact gatttatgtc
    39721 gccccgtgga ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt gttggttaac
    39781 ctccttggcg atggcgttcg tcgtgcgatt attgcggggg tggaataatg ccattactcg
    39841 atattcgtaa cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa gccgtcgacc
    39901 gggtaagtat gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa tccggttccg
    39961 gcaagagttt gattgcgaaa gcgatttgtg gggtgaataa agataactgg cgtgttactg
    40021 ctgaccgtat gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc gaacggcgca
    40081 aactggttgg ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt cttgaccctt
    40141 cagaacgtgt gggtcgccag ttgatgcaaa acatcccagc ctggacctat aaaggccgtt
    40201 ggtggcagcg ttttggctgg cgcaaacgcc gtgccattga actgctgcac cgcgtgggga
    40261 ttaaagatca caaagatgcg atgcgcagtt ttccctatga gttgaccgaa ggtgaatgtc
    40321 agaaagtgat gatagccatt gcactggcga atcaaccgcg tctgctgatt gctgacgaac
    40381 cgaccaactc aatggagcca acaacccagg cgcaaatctt tcgcctgctg acgcgtctca
    40441 accaaaacag caataccact attttgctta tcagccatga cttacaaatg cttagccaat
    40501 gggcggataa aattaacgtg ctttactgcg gtcaaacagt ggaaaccgcg ccgagtaagg
    40561 agttggtgac gatgccacat catccttata cccaggcgct gatccgcgcg ataccagact
    40621 tcggcagcgc gatgccgcat aaaagtcgcc tcaatacgct gcccggcgct atcccactgc
    40681 tggaacagtt accgattggt tgtcgtctgg ggccacgttg cccgtatgca caacgagaat
    40741 gcattgtgac gccacgtttg acgggggcga aaaatcatct ctatgcctgt catttcccgc
    40801 tgaacatgga gaaagagtga gatgatcgaa acgctgcttg aagtgcgtaa tttaagtaaa
    40861 acgttccgct accggaccgg ctggtttcgt cgtcagaccg tagaagcggt aaaacccttg
    40921 agctttacgc tacgtgaagg ccagacactg gcgattattg gcgagaatgg ttcgggtaaa
    40981 tccacgctgg caaaaatgct ggcgggaatg atagagccta ccagcggcga gttattgatt
    41041 gacgatcatc cactgcattt tggcgattat tccttccgca gtcagcgtat tcgcatgatt
    41101 tttcaggatc cttcgacctc gttgaatccc cgtcaacgta tctcgcaaat tctggatttt
    41161 ccactgcgcc tgaacaccga cctggaaccg gaacagcgtc gcaaacagat tattgaaacg
    41221 atgcgtatgg ttgggctgct accggatcac gtcagttact atccgcatat gttggcaccc
    41281 ggacaaaaac agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa agtcattatt
    41341 gccgatgaag ccctcgcttc actggatatg tcgatgcgtt cgcagttgat taatctgatg
    41401 ctggagttac aggaaaaaca gggcatttcg tatatttatg ttacccagca tatcggaatg
    41461 atgaagcaca ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt cgaacgaggc
    41521 agcaccgcgg atgtgctggc atcgccgctg catgaactca ccaaacggct gattgccggg
    41581 cactttggcg aagcattgac agcggatgcg tggcgtaaag atcgctaatt ttctgaatcc
    41641 tccttccaga ttatccacct cattctgaca agagattaaa tcttgtcaga atgaggtgat
    41701 ggataaaata aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc aggaacgaaa
    41761 gctctggcga tatcttcgca gccgacgttt tggtgatttc aaatttcgcc gtcaacatcc
    41821 ggtggggagc tacattctcg attttgcttg ctgctcggcg cgcgtagtcg ttgagctgga
    41881 tggtgggcag catgatttgg cagttgccta tgataccagg cgcactagct ggcttgagtc
    41941 gcagagctgg accgtgctgc gtttctggca taacgagatt gattgtaatg aggaggcggt
    42001 gctggagatt attctgcagg aactgaaccg ccggtcaccc tcaccctgaa agggcgaggg
    42061 ggccgaccga gccgaatagc tgttgtggtg aaaacatgga gacagtgctg gagaatattc
    42121 ggcaaggtct gaaccatccc agccatcgcc ataaaagggt taggggctgt atgagcctgt
    42181 ttgttgctgg ggtaacaata tttgcacaat atggtcccct cgcccctctg gggagagggt
    42241 tagggtaagg ggaaaagcgc ccaccctgcc gcagcctgct ccggtcggac ctggcaacta
    42301 tagctactca cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa
    42361 acgacgaatc gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg
    42421 gttttctttc cggtaagcgc attctggtaa ccggtgttgc cagcaaacta tccatcgcct
    42481 acggtatcgc tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg
    42541 acaaactgaa aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc
    42601 agtgcgatgt tgcagaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt
    42661 ggccgaaatt tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg
    42721 gtgactatgt taacgccgtt acccgtgaag gcttcaaaat tgcccacgac atcagctcct
    42781 acagcttcgt tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc
    42841 tgaccctttc ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg
    42901 caaaagcgtc tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg
    42961 tgcgtgttaa cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag
    43021 acttccgcaa aatgctggct cattgcgaag ccgttacccc gattcgccgt accgttacta
    43081 ttgaagatgt gggtaactcc gcggcattcc tgtgctccga tctctctgcc ggtatctccg
    43141 gtgaagtggt ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaattga
    43201 aataatcatt ctgttggtaa atattgggcg gcgttctgcc gcccgttatc tctgttatac
    43261 ctttctgata tttgttatcg ccgatccgtc tttctcccct tcccgccttg cgtcaggata
    43321 acgatttcct ttacgaccaa ggagcgccca tggaacaacg ccacatcacc ggcaaaagcc
    43381 actggtatca tgaaacgcaa tccagtactg cggagtatga cgttctgcct ctggtcccgg
    43441 aagccgcaaa ggtcagcgat ccctttctgc tcgacgtgat ccttgatgaa gaaacgctgg
    43501 ctcccttcct ttcatggctg gtccctgcgc gcgttcttgc agtggaattg ttccctgacc
    43561 agcttaccgt gacccgttca cagaccttca ccgcttatga acgcttgtct acggccctga
    43621 cggttgctca ggtttgcggc gtccagcggt tatgtaacta ctattcggcg cgacttacgc
    43681 cgctccccgg gcctgattcc tccagggaaa gcaatcatag gttggcacaa atcacgcaat
    43741 atgcccgcca actggctagc tcgccttcta ttatcgacaa ccgatcgcgc cagcatctga
    43801 atgacgtcgg tcttactgcc ggggactgtg tgatcattaa ccaaatcatt ggttttattg
    43861 gctttcaggc gcggaccatt gcgacatttc aggcttatct cgggcacccg gtacgctggt
    43921 tacccggtct ggaaatacaa aactacgccg acgcgtcact gtttgctgat gaatcaatac
    43981 gctggcgaag cagctatgag gtggaaaaac tacctgaaga gtacacaaaa agttcaacag
    44041 cagaactttg ccaactggcc gaaacactct ctctccaccc tatttcactt tcccttctcg
    44101 aaaggttgtt aaacagtaca cgggttaata cacagccgga taataagctt gcggcgttgt
    44161 tatgcgcgcg gataaatggc agtcctgctt gttttgccgc ctgtatggat tcatcaaatg
    44221 aatataaaaa aatcagcccc cttctgcgca agggcgaaaa tgaaattaac caatgggctg
    44281 accgtcattc tgttgagcac gctaccgttc aggcgataca atggctgacc cgagcacccg
    44341 atcgctttag cgccgcccag ttcagccctt tactcgaaca cgaaaaatca tcaacgcaga
    44401 ttattaatct gctggtatgg agcgggctgt gtggctggat aaatcgctta aaaatcgcgt
    44461 tgggtgagac atattaacct tgccgcgtca gacagattcg cgtaaaactg tcagccgctc
    44521 taatggccac caaaatagac aattatgttt caggacaacc cgctgctagc gcagcttaaa
    44581 cagcaactgc attcccagac gccacgcgct gaaggggtgg taaaagccac agaaaaaggc
    44641 tttggcttcc tggaagtcga cgcgcaaaaa agttatttca ttccgccgcc gcagatgaaa
    44701 aaagtcatgc atggcgaccg aattatcgcg gtgatccaca gtgaaaaaga acgtgaatcc
    44761 gcagagccag aagaactggt tgaaccgttc ctgactcgtt tcgtgggtaa agttcagggc
    44821 aaaaatgacc gtctggccat cgttcctgat catccactct taaaagacgc cattccttgc
    44881 cgcgcagccc gtggcctgaa ccacgagttt aaagaaggcg actgggcggt tgccgaaatg
    44941 cgccgtcatc cgctgaaagg cgatcgttct ttctatgctg aactgacaca atacatcact
    45001 tttggtgacg accactttgt accgtggtgg gttacccttg cgcgccataa tctggaaaaa
    45061 gaagcaccag acggcgtcgc taccgcaatg ctcgatgaag gtctggttcg tgaagatctg
    45121 accgcgctgg attttgtcac catcgacagt gccagcacag aagatatgga tgacgccctt
    45181 ttcgctaagg cgttgccgga tgacaaactt cagctgattg tggcgattgc cgatccaacc
    45241 gcgtggattg ctgaaggcag caagctggac aaagccgcga aaattcgcgc attcaccaac
    45301 tatctgcctg gcttcaacat ccctatgctg cctcgcgagc tttctgacga tctctgctca
    45361 ctgcgcgcca atgaagtccg cccggtactg gcatgccgca tgacgctctc cgctgatggc
    45421 accattgaag ataatatcga attctttgcc gccaccatcg aatccaaagc aaagctggtg
    45481 tatgaccagg tttctgactg gctggaaaat accggtgact ggcagcctga aagtgaagca
    45541 attgccgaac aagtccgttt gctagcgcaa atttgccaac gccgcggcga gtggcgtcat
    45601 aaccacgcac tggtgtttaa agatcgcccg gattaccgct ttattctcgg tgaaaaaggt
    45661 gaagtgctgg atatcgtcgc cgagcctcgt cgcattgcca accgtatcgt cgaagaagcg
    45721 atgattgccg ctaacatttg tgcggcccgc gtactgcgcg ataagctcgg ttttggcatc
    45781 tataacgtgc atatgggctt tgatccggcg aatgccgacg cgctggcagc gttgctgaaa
    45841 acgcacggtc tgcatgtcga tgccgaagaa gtgctcacgc tggacggttt ctgcaaactg
    45901 cgtcgtgaac tggacgcgca accaactggt ttcctcgaca gccgcattcg tcgcttccag
    45961 tcatttgctg aaattagcac tgaacccggt cctcactttg gcctcggtct ggaagcatac
    46021 gccacctgga cttcgccgat ccgtaaatat ggcgacatga tcaaccaccg tctgctgaaa
    46081 gcggttatca aaggcgaaac tgcgacgcgc ccacaggatg aaatcactgt ccaaatggcc
    46141 gagcgtcgcc gtctcaaccg gatggcagaa cgtgatgttg gtgactggtt atacgcacgc
    46201 ttcctgaaag acaaagccgg gaccgacacc cgtttcgcag cggaaattgt cgatatcagc
    46261 cgtggcggca tgcgtgttcg tttggttgat aacggcgcta tcgcctttat tccggcacct
    46321 ttcttacacg ctatgcgcga tgaactggtt tgcagccagg aaaacggcac tgtacaaatt
    46381 aaaggtgaaa cggtttacaa agtgactgac gttattgacg tcactattgc cgaagtccgc
    46441 atggaaaccc gcagcattat tgcgcgcccg gtcgcgtaat ctcctttcac ggcccattcc
    46501 tcatggatgg gccgtttatt tccccgctct accttcgtta tatccaagca gattttttaa
    46561 ctgactttcg tttgaaaact ggcgtttttc caaaactgga ttacaaataa atatataaaa
    46621 aaaacaatgc gtttagccgc cgttaatttc atgtaagtca tgttgttcct tttatttaac
    46681 cgcggactcc gcagttaacc ggaggatatg catcatgaaa accgttaggg agtccacaac
    46741 gttgtacaac tttctcggat cgcacaatcc atactggcgg ttgacggaaa gcagcgatgt
    46801 tttgcgcttt tctaccaccg aaaccacaga acctgatcgt acattgcagt tatctgccga
    46861 acaggctgct cgcatcaggg aaatgacggt catcacctcc agcctgatga tgagtctgac
    46921 cgtcgatgaa agcgatcttt ctgtgcatct ggtaggacga aaaatcaata aacgggaatg
    46981 ggctggcaac gcgtctgcat ggcatgacac accggcggtt gctcgtgatt tatcacacgg
    47041 gctttccttt gctgagcagg tagtttctga agcacattcc gcaatagtga ttctcgacag
    47101 ccgggggaat atccaacgct tcaatcggtt atgtgaagat tacacagggt tgaaagaaca
    47161 cgacgtcatt gggcaaagcg tgtttaaact gtttatgagc cgtcgtgaag ctgcggcatc
    47221 caggcgcaat aaccgtgtat tttttcgaag cggcaatgca tatgaagtcg aactgtggat
    47281 accaacatgt aaaggccagc ggctgtttct gtttcgcaat aaatttgtcc acagcggcag
    47341 tggcaaaaac gagatttttt taatctgttc cggcaccgac attaccgaag agcgccgcgc
    47401 tcaggagcga ctgcgtattc tggcaaatac cgacagtatc accggactgc cgaatcgtaa
    47461 cgcaatgcag gatttaatcg atcacgctat taatcatgca gataacaata aagttggggt
    47521 tgtgtatctt gatttggata atttcaaaaa ggtcaacgac gcctatgggc atttgtttgg
    47581 tgaccagtta ttacgcgacg tgtcattggc tattttaagc tgtctcgaac atgaccaggt
    47641 gttggcgcgt ccaggtgggg atgagtttct ggtactggca tccaacacct cacaaagcgc
    47701 gctggaagca atggcatcac gaattttgac ccgcttacgg ctcccctttc gcattggttt
    47761 aattgaagtt tataccagct gttcagtagg tattgcactc tctcccgaac atggttcaga
    47821 cagcacggct attattcgtc acgccgacac agcaatgtac acagcgaagg aaggcggacg
    47881 aggacaattt tgcgttttta ccccagaaat gaatcaacgg gtatttgaat atctctggct
    47941 ggataccaac ttgcgtaaag cactggaaaa cgatcagttg gttattcact atcaaccgaa
    48001 aatcacctgg cgtggcgaag tgcgcagtct ggaagcacta gtacgttggc agtcacctga
    48061 acgtgggttg attccaccgt tggacttcat ttcctacgcc gaagagtcag ggctaattgt
    48121 gcctttaggc cgttgggtga ttctcgatgt cgtacgccag gtggcaaagt ggcgggaaaa
    48181 aggcataaac ctgcgagtgg cggtaaatat ttctgcacgt cagctcgccg atcaaaccat
    48241 tttcaccgcc ctgaaacagg ttctccagga actcaatttt gaatactgcc ctatagatgt
    48301 tgaactgaca gagagttgtc tgattgagaa tgatgaactg gcactgtctg ttattcaaca
    48361 atttagccaa ctaggtgcgc aagtgcatct ggatgatttt ggtaccggct actcttcact
    48421 ttcgcaactg gcgcgctttc cgatcgatgc catcaaactt gaccaggttt ttgttcgaga
    48481 tattcacaaa caacctgtct cgcagtcact ggtccgggcg atcgtcgctg tggcccaggc
    48541 attgaatctt caggtgatcg ccgaaggtgt agagagtgca aaggaagatg cttttttaac
    48601 caagaacggg atcaatgagc ggcaaggatt tttgtttgcc aaaccgatgc ccgccgtcgc
    48661 cttcgaacgc tggtataaac gctatctgaa gcgcgcataa gtagatgaat atatatactt
    48721 agcgctatat cccgtcatta tatatcgtat tcctggatga taattagccg ttgctgttaa
    48781 acacttctct tttagcggca atttttgcat tattcatttt cccccttttg ctataaggat
    48841 gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata ttgtgttaga
    48901 tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac ttaaagcaga
    48961 actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt gggagattta
    49021 acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta tttttttatt
    49081 tagtattata acgttataag aattacagcg atgaattccc gacaacaaac tattctacag
    49141 atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac tggagtttct
    49201 gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct ccgccgtgca
    49261 catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat gagcaactat
    49321 acgctgaagc gtgaactcgc cgagtttgcc gcgtctctgg ttcaaccggg cgaaaccatc
    49381 tttatcgaaa atggtagcag taatgccctg ctcgcccgga ctctgggcga acagaagaaa
    49441 aatgtcacta tcatcacagt cagcagctac attgcgcatt tgctaaaaga cgcgccttgt
    49501 gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt tggccctttg
    49561 acacgccagt gcatccagca ggtacatttc agcaaagcat ttattggtat tgatggctgg
    49621 caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt caatgccgtg
    49681 ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg tgctgtacat
    49741 tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc gaaaatacgc
    49801 gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt tgacatttaa
    49861 ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct aagatgattc
    49921 ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata aaattattat
    49981 cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg cgagcagatt
    50041 tcacggaata atttcaccag gcttattctt agctattata gttatagaga gcttacttcc
    50101 gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa tgaccgcggc
    50161 tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt ctaaacggga
    50221 ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac tgaccgatgg
    50281 cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc accaggtcgg
    50341 taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct tgattctgtt
    50401 cagtacgaaa taactgaata atacacacag taaaatattc gaaagccacg gatatatccg
    50461 tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt caagtagtga
    50521 ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa ctgctccgcc
    50581 gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat catcgagatc
    50641 gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac gctgaatacg
    50701 caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa tacgtccggt
    50761 ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg ctgtaaagag
    50821 gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg gcgaccaatc
    50881 accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg gcgctggtca
    50941 ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc ctgaccgaat
    51001 acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag gccacatttt
    51061 tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat gccaagatcg
    51121 accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag cggtgcatct
    51181 ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc accagaggca
    51241 tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg cgcagcagtg
    51301 ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg ttgaagttcg
    51361 cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag acgacaatcg
    51421 cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata atcaagggca
    51481 acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa cgtcatgacc
    51541 agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc gagcattcta
    51601 cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt ttctgcgccg
    51661 tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa gtaggatttt
    51721 ttaaaaatta caggccatca agaccgcgaa tcggtttaat ggttgaccag gcccgacaag
    51781 acggacaatg ccagtagagg gtgtatgcgg taaaaccaca tttctggcag cgataacgag
    51841 gcttactacg taccttttcg ccaaccatgt cacgcagcac catcaggctc tctttggcac
    51901 gcccttcttc cgcttcattt aagtggtaat ccattaactt atggaacaca cgcatggtcg
    51961 gatgacgctg aagctggcgc gtaatataga cctgtgcggc ctcactaccg tcgcgcgctt
    52021 cgatgatatc agcaagcatc aattcagcat cggcaccggt gttctcttcc accgcgcgtt
    52081 gcaggaattc tgcccattcg gcagttttac ccaactgctg atagcaggtt tgcaacattt
    52141 ccagcgtttc gctgaccagt tctctgtcct gggatatcac gcgttgcaaa ctttcgacgg
    52201 ctttggcgta ttctcctttc gccataaaca cgcgtcccat catgatggat acgcgggcgc
    52261 tgtttttatc tgccgccgcc ccttttttta gcaaggtcat ggcacgatcg agatcgtcgc
    52321 tggccatatg ctgcagggct aactcacagt agaaatgggc aatttcgacg cgctgtttat
    52381 ctttacccag cttcaccagg cgttcggcaa catcaattgc tttctgccac tcactggtag
    52441 cctggtagat ttgtagcaac tgttgcagag cgccaatgcg gaagtcagtt tcatcggtca
    52501 gctgattgaa catgtcttcc gcgcggtcgt ataacccggc ggccatgtaa tcacgcccta
    52561 gttgttgaat cgccaacaga cgctgttcat aggtcagcga ggcgctttcc attagggtct
    52621 gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg gaacaggttt ccgagcgtaa
    52681 ggtgggcttc aacggtgcct gtatcctctt taagcatatc gagaaacagg tctaccgctt
    52741 tatcctgttg attactaagc aggaagttaa cccccgctac gtaatcacgc gacaagcggt
    52801 tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc catataccag ccataggccg
    52861 cggctacagg caaaagcaga aacaacaact ccagcataga aagttattcc ttcgccgctg
    52921 acgagtgcgg cactacagcc acgtcagtcg cgggtgaaag ctggttttcc agtcgcttta
    52981 ttttacgttc agcgcgcgcc agggaaacac gaactcgcag ccagaacagg ccacaaatca
    53041 accaaccgat agcaaacccc gcagcaaaca ataccgccag caatgtggaa atacggtact
    53101 ccccttgcgc taacagataa ttaaacgtca cctgttgatc gttctgcgca cccaacgtga
    53161 ccgaaatcac gaagatcgct aacaccagta agaaaatgag taaatatttc acattacttc
    53221 ccgttatgtg gttagagcga ataaattatg gtcaacttac cgcaatagcc ctttaaagta
    53281 ccattttcgc gccgtctgcg aaatggcatc cacgcgcatt gttgatgatt taagggctaa
    53341 attcggaaaa tcaaccagcg ttaactttct tgttctcgtt gcgctatttc gcgattttct
    53401 tccgcaggtg gtgttaatgg cccacaaatt cgttgcgcaa gccacgttgc caccgccacc
    53461 agcgcccacg aaatcaacgt agctactacc agatcgcgtg gccaatgcat cccgagcagc
    53521 aggcggcttc ccatgactcc cgttgcccag accagcaaga tagcaatggt taacgttcgc
    53581 cgacgcggcc acagcaaacc aacggccagc agtgcccaac tggcagcaaa catcgtgtga
    53641 ccggaaggaa aggcaaaccc cgtctctttc tgccaatgtg aacgcaaata ttgtgggata
    53701 tttttctctt cagccaactg ttctttcact agatttccgc gttctgctcg ctttaaagtg
    53761 tagaactcat caaccggaat atgatgtgtt ttttccagcc agataacaaa aggtcgtggt
    53821 tcctggactt tgtctttgat ccaggattta acgccttgtc ccacaaggat tgcggccgcc
    53881 agaatggcaa ataatacaaa ggcagcctta atgcgaaaac gcagacacca gagaaaccag
    53941 ccgaataaaa tcaaatgtgt aatgacgccc cagggctggg tgacagtttc agtaacccaa
    54001 aaagccgctt ttagtagcca actttgttct ccaggttgcc aacgccagcc agaaatccat
    54061 acggctactg gcatgacaag caatagtgca gctcccactg cggtacgtct ggcaatcgaa
    54121 cgcatggcct ctcctttttg ataagtccca caatcataac tgaaaacgcc agttccagga
    54181 aaaattgaca gatttgtgcc attccgtgaa cgatcgacgc gtcgtgatta ggtgaacccc
    54241 ttctcgttat ggcaaaataa gccaatacag aaccagcatt atctggagaa tttcatgcag
    54301 cttaaacgtg tggcagaagc caaactgcca accccatggg gcgatttcct gatggtggga
    54361 tttgaagaac tggcaaccgg acacgatcat gtcgcgctag tctatggcga tatttccggg
    54421 cataccccgg tacttgcgcg cgtccattcc gaatgtctga ccggtgacgc cctgttcagc
    54481 ttgcgctgcg attgtggctt ccagctcgaa gcggcattga cgcaaattgc cgaggaaggc
    54541 cgtggtattt tgctgtatca ccgtcaggaa ggtcgtaaca ttggtctgct gaataaaatc
    54601 cgcgcttacg cactgcagga tcaaggttac gataccgtag aggctaacca ccagttaggc
    54661 ttcgccgctg atgagcgcga cttcactctt tgcgctgata tgttcaaact ccttggcgtc
    54721 aatgaagtcc gcttgttaac caataacccg aaaaaagtcg aaattctgac cgaagcaggg
    54781 attaatattg ttgaacgcgt accattgatt gtaggtcgta accccaataa cgaacattat
    54841 ctcgatacca aagccgagaa aatgggccat ttgctgaaca aataaccctc ttgcattgtg
    54901 taattaattt gcttgccgga agcaaaataa ccggcaagca aatacttgtt acttcaacat
    54961 attacgaatg acataatgca aaatgccgtc gttctggtag taggtcaact ccgtcgcggt
    55021 gtcgatacga caacggcagg gtacgacctc ctggctacca tccgcgcgcg taagcgtcac
    55081 cggaaccgtc gcaccgggtt gtagattttg cagatcgcca atatcaatct tctcttcccc
    55141 ggttagtcct aacgttttac gcgttacgcc ttgcggaaat tccagcggca ggatgcccat
    55201 gccaattaaa ttcgaacggt gaattcgttc aaacgattcg gcaatcacca cacgaattcc
    55261 aagcagacga ggaccttttg ccgcccagtc acggcttgag cctgatccat actctttccc
    55321 ggcaatcacc gccagcggcg tttgttcctt cttatagcgc atcgcagcat cataaataga
    55381 gactacgtcg ctgtcaggta aatgccgcgt catcccccct tcaacgccag gcaccatttc
    55441 attacggatg cgaatattgg cgaaggtgcc gcgcatcatc acttcatggt taccacgccg
    55501 cgaaccgtag gagttaaagt cttttcgctc aacaccccga ccttgtagat atcgacccgc
    55561 tgggctgtcg ggcttaatac tgcccgccgg agagatatgg tcagtggtga ctgaatcccc
    55621 cagcattgcg aggatccgcg caccgtgaat atcttccact ggtgctggtg ttgcctgcat
    55681 ttcatcaaag aaaggcgata agcgaatata ggttgagtcc tcctgccaac cgtaggtatc
    55741 ggatcgtgtg acgttaattt ccttccactc tgctgtgcct tcaaaaactt ctgcgtactc
    55801 tttgcggaac atttctgtgg agacttgttc taccgcacgg gcaatttctt gtgccgatgg
    55861 ccagatatct ttcagataaa ccgggtcgcc tttacgatca tgaccaatag gctcagcagc
    55921 caggttgata ttcatatttc ccgccagcgc ataggcaacc accagcggcg gcgaggccag
    55981 ccagttagtt ttaaccagcg gatggatacg gccttcaaag ttacggttgc cggacagcac
    56041 cgcaccgacg gttaaatcgc cttttttgat tgccgtttcg ataggatcgg gcagcggccc
    56101 agagttacca atacaggtgg tacaaccgta tcccacaagg ttaaacccca gttcgtcgag
    56161 atacggtgtc agtttcgctt ttgccagata atcagaaacg actttcgaac ccggtgccag
    56221 cgacgctttg acccacggtt gccgcttgag gcccagagtt acggcttttt tcgccagcaa
    56281 gcctgcggcc atcagcacac ttgggttaga ggtgttggtg cacgaggtta tcgcagcaat
    56341 gaccacagcg ccatcaggta actgatactg atgtccgttc ataacataat cgaccggctg
    56401 gcgatcttta tgcgtggcat tcacttccag ttcgttactg gcggcaaatg cttttggtac
    56461 atcgggcagt gcaacgcgat cctgtgggcg ttttggccct gccaggctcg cttcaacgtc
    56521 attcatatcc agttctaacg tactggtaaa aatcggttca tcgcccgggt tacgccacat
    56581 gccctgcgct ttggcatatt tttcgaccaa ctcaacctga tcttcgctgc gcccgcttaa
    56641 acgcatgtaa tcgagggtta cagcatcgat tgggaagaag ccacaggtgg caccatattc
    56701 tggcgacata ttggcaatgg tggcgcgatc cgccagcggt agtgaatcca gaccatcacc
    56761 ataaaattcg acgaatttcc ccaccacgcc atgtttgcgc agcatttggg taacagtgag
    56821 aaccaggtct gtggcggtaa taccttcacg taatttaccg gtaagtttaa agcccactac
    56881 gtccgggata agcatggaaa ccggctggcc
//
LOCUS       DAZGOB010000016        56186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGOB010000016 DAZGOB010000000
VERSION     DAZGOB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.016"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..281)
                     /gene="tccP2"
                     /locus_tag="ACHLIF_001012"
     CDS             complement(<1..281)
                     /gene="tccP2"
                     /locus_tag="ACHLIF_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFO9605070.1"
                     /translation="MINSINSFFSSIPHSISSVTRNSSFTASQHKSTPNTVKTSSPLS
                     PSNSPASATTIFKVKNSYTESGLQRSTSYTQSSIEKNALHRPLPDVAKRL"
     gene            complement(727..1131)
                     /locus_tag="ACHLIF_001013"
                     /pseudo
     CDS             complement(727..1131)
                     /locus_tag="ACHLIF_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
     gene            complement(1214..1301)
                     /locus_tag="ACHLIF_001014"
     tRNA            complement(1214..1301)
                     /locus_tag="ACHLIF_001014"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1265..1267),aa:Ser,seq:tga)
     gene            1728..2846
                     /gene="hyaA"
                     /locus_tag="ACHLIF_001015"
     CDS             1728..2846
                     /gene="hyaA"
                     /locus_tag="ACHLIF_001015"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO9605071.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2843..4636
                     /gene="hyaB"
                     /locus_tag="ACHLIF_001016"
     CDS             2843..4636
                     /gene="hyaB"
                     /locus_tag="ACHLIF_001016"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO9605072.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4655..5362
                     /gene="hyaC"
                     /locus_tag="ACHLIF_001017"
     CDS             4655..5362
                     /gene="hyaC"
                     /locus_tag="ACHLIF_001017"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO9605073.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5359..5946
                     /gene="hyaD"
                     /locus_tag="ACHLIF_001018"
     CDS             5359..5946
                     /gene="hyaD"
                     /locus_tag="ACHLIF_001018"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO9605074.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            5943..6341
                     /gene="hyaE"
                     /locus_tag="ACHLIF_001019"
     CDS             5943..6341
                     /gene="hyaE"
                     /locus_tag="ACHLIF_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO9605075.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6338..7195
                     /gene="hyaF"
                     /locus_tag="ACHLIF_001020"
     CDS             6338..7195
                     /gene="hyaF"
                     /locus_tag="ACHLIF_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO9605076.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            7329..8874
                     /gene="appC"
                     /locus_tag="ACHLIF_001021"
                     /pseudo
     CDS             7329..8874
                     /gene="appC"
                     /locus_tag="ACHLIF_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
     gene            8886..10022
                     /gene="appB"
                     /locus_tag="ACHLIF_001022"
     CDS             8886..10022
                     /gene="appB"
                     /locus_tag="ACHLIF_001022"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO9605077.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPSAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            10035..10127
                     /gene="cbdX"
                     /locus_tag="ACHLIF_001023"
     CDS             10035..10127
                     /gene="cbdX"
                     /locus_tag="ACHLIF_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO9605078.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10207..11505
                     /gene="appA"
                     /locus_tag="ACHLIF_001024"
     CDS             10207..11505
                     /gene="appA"
                     /locus_tag="ACHLIF_001024"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO9605079.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11620..13800)
                     /gene="etk"
                     /locus_tag="ACHLIF_001025"
     CDS             complement(11620..13800)
                     /gene="etk"
                     /locus_tag="ACHLIF_001025"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO9605080.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13820..14266)
                     /gene="etp"
                     /locus_tag="ACHLIF_001026"
                     /gene_synonym="yccY"
     CDS             complement(13820..14266)
                     /gene="etp"
                     /locus_tag="ACHLIF_001026"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO9605081.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(14254..15393)
                     /gene="gfcE"
                     /locus_tag="ACHLIF_001027"
     CDS             complement(14254..15393)
                     /gene="gfcE"
                     /locus_tag="ACHLIF_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO9605082.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15439..17535)
                     /gene="gfcD"
                     /locus_tag="ACHLIF_001028"
     CDS             complement(15439..17535)
                     /gene="gfcD"
                     /locus_tag="ACHLIF_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO9605083.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17535..18281)
                     /gene="gfcC"
                     /locus_tag="ACHLIF_001029"
     CDS             complement(17535..18281)
                     /gene="gfcC"
                     /locus_tag="ACHLIF_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO9605084.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(18278..18922)
                     /gene="gfcB"
                     /locus_tag="ACHLIF_001030"
     CDS             complement(18278..18922)
                     /gene="gfcB"
                     /locus_tag="ACHLIF_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO9605085.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(19029..19334)
                     /gene="gfcA"
                     /locus_tag="ACHLIF_001031"
     CDS             complement(19029..19334)
                     /gene="gfcA"
                     /locus_tag="ACHLIF_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFO9605086.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19776..19988)
                     /gene="cspH"
                     /locus_tag="ACHLIF_001032"
     CDS             complement(19776..19988)
                     /gene="cspH"
                     /locus_tag="ACHLIF_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO9605087.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            20274..20486
                     /gene="cspG"
                     /locus_tag="ACHLIF_001033"
     CDS             20274..20486
                     /gene="cspG"
                     /locus_tag="ACHLIF_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO9605088.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20497..20685
                     /gene="ymcF"
                     /locus_tag="ACHLIF_001034"
     CDS             20497..20685
                     /gene="ymcF"
                     /locus_tag="ACHLIF_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO9605089.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20660..20890
                     /gene="ymcE"
                     /locus_tag="ACHLIF_001035"
     CDS             20660..20890
                     /gene="ymcE"
                     /locus_tag="ACHLIF_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO9605090.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            20880..21053
                     /gene="gnsA"
                     /locus_tag="ACHLIF_001036"
     CDS             20880..21053
                     /gene="gnsA"
                     /locus_tag="ACHLIF_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO9605091.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(21101..22174)
                     /gene="yccM"
                     /locus_tag="ACHLIF_001037"
     CDS             complement(21101..22174)
                     /gene="yccM"
                     /locus_tag="ACHLIF_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO9605092.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(22246..24990)
                     /gene="torS"
                     /locus_tag="ACHLIF_001038"
     CDS             complement(22246..24990)
                     /gene="torS"
                     /locus_tag="ACHLIF_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO9605093.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            25073..26101
                     /gene="torT"
                     /locus_tag="ACHLIF_001039"
     CDS             25073..26101
                     /gene="torT"
                     /locus_tag="ACHLIF_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO9605094.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(26074..26766)
                     /gene="torR"
                     /locus_tag="ACHLIF_001040"
     CDS             complement(26074..26766)
                     /gene="torR"
                     /locus_tag="ACHLIF_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO9605095.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26896..28068
                     /gene="torC"
                     /locus_tag="ACHLIF_001041"
     CDS             26896..28068
                     /gene="torC"
                     /locus_tag="ACHLIF_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO9605096.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            28068..30614
                     /gene="torA"
                     /locus_tag="ACHLIF_001042"
     CDS             28068..30614
                     /gene="torA"
                     /locus_tag="ACHLIF_001042"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO9605097.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            30611..31210
                     /gene="torD"
                     /locus_tag="ACHLIF_001043"
     CDS             30611..31210
                     /gene="torD"
                     /locus_tag="ACHLIF_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO9605098.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVGRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(31303..31608)
                     /gene="cbpM"
                     /locus_tag="ACHLIF_001044"
     CDS             complement(31303..31608)
                     /gene="cbpM"
                     /locus_tag="ACHLIF_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO9605099.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(31608..32528)
                     /gene="cbpA"
                     /locus_tag="ACHLIF_001045"
     CDS             complement(31608..32528)
                     /gene="cbpA"
                     /locus_tag="ACHLIF_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO9605100.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            32788..34047
                     /locus_tag="ACHLIF_001046"
     CDS             32788..34047
                     /locus_tag="ACHLIF_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFO9605101.1"
                     /translation="MSSNIHGISCTANNYLKQAWNNIKNEHEKNQKYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECDSLEKEFEEMQNDNDLSLFLRTLRTNDTETYSGVSE
                     GITYTIQYVRDIDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEWINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFNDLREDVKTLFGVNLQS
                     DNKIQGQGGFLSWSFLSFKKEFIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEQKV
                     NEVKNFLTALSDSICSLFIVNGNVASISLGNESFSTDDDLEYGYLINTGNHYDVYLPP
                     ELFAQAYELNNKERNAQIDFLTRYAIY"
     gene            34339..35580
                     /gene="agp"
                     /locus_tag="ACHLIF_001047"
     CDS             34339..35580
                     /gene="agp"
                     /locus_tag="ACHLIF_001047"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO9605102.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(35618..35845)
                     /gene="yccJ"
                     /locus_tag="ACHLIF_001048"
     CDS             complement(35618..35845)
                     /gene="yccJ"
                     /locus_tag="ACHLIF_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO9605103.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(35866..36462)
                     /gene="wrbA"
                     /locus_tag="ACHLIF_001049"
     CDS             complement(35866..36462)
                     /gene="wrbA"
                     /locus_tag="ACHLIF_001049"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO9605104.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            36835..37008
                     /gene="ymdF"
                     /locus_tag="ACHLIF_001050"
     CDS             36835..37008
                     /gene="ymdF"
                     /locus_tag="ACHLIF_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO9605105.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(37091..38419)
                     /gene="rutG"
                     /locus_tag="ACHLIF_001051"
     CDS             complement(37091..38419)
                     /gene="rutG"
                     /locus_tag="ACHLIF_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO9605106.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(38440..38934)
                     /gene="rutF"
                     /locus_tag="ACHLIF_001052"
     CDS             complement(38440..38934)
                     /gene="rutF"
                     /locus_tag="ACHLIF_001052"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO9605107.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(38945..39535)
                     /gene="rutE"
                     /locus_tag="ACHLIF_001053"
     CDS             complement(38945..39535)
                     /gene="rutE"
                     /locus_tag="ACHLIF_001053"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO9605108.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(39545..40345)
                     /gene="rutD"
                     /locus_tag="ACHLIF_001054"
     CDS             complement(39545..40345)
                     /gene="rutD"
                     /locus_tag="ACHLIF_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO9605109.1"
                     /translation="MKLSLSPPPYADTPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(40353..40739)
                     /gene="rutC"
                     /locus_tag="ACHLIF_001055"
     CDS             complement(40353..40739)
                     /gene="rutC"
                     /locus_tag="ACHLIF_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO9605110.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(40751..41443)
                     /gene="rutB"
                     /locus_tag="ACHLIF_001056"
     CDS             complement(40751..41443)
                     /gene="rutB"
                     /locus_tag="ACHLIF_001056"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO9605111.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(41443..42534)
                     /gene="rutA"
                     /locus_tag="ACHLIF_001057"
     CDS             complement(41443..42534)
                     /gene="rutA"
                     /locus_tag="ACHLIF_001057"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO9605112.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            42822..43460
                     /gene="rutR"
                     /locus_tag="ACHLIF_001058"
     CDS             42822..43460
                     /gene="rutR"
                     /locus_tag="ACHLIF_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO9605113.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            complement(43500..47462)
                     /gene="putA"
                     /locus_tag="ACHLIF_001059"
     CDS             complement(43500..47462)
                     /gene="putA"
                     /locus_tag="ACHLIF_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9605114.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPLSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            47517..47726
                     /locus_tag="ACHLIF_001060"
     CDS             47517..47726
                     /locus_tag="ACHLIF_001060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605115.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            47885..49393
                     /gene="putP"
                     /locus_tag="ACHLIF_001061"
     CDS             47885..49393
                     /gene="putP"
                     /locus_tag="ACHLIF_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO9605116.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            50059..50889
                     /gene="efeU"
                     /locus_tag="ACHLIF_001062"
     CDS             50059..50889
                     /gene="efeU"
                     /locus_tag="ACHLIF_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO9605117.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            50947..52074
                     /gene="efeO"
                     /locus_tag="ACHLIF_001063"
     CDS             50947..52074
                     /gene="efeO"
                     /locus_tag="ACHLIF_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO9605118.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            52080..53351
                     /gene="efeB"
                     /locus_tag="ACHLIF_001064"
     CDS             52080..53351
                     /gene="efeB"
                     /locus_tag="ACHLIF_001064"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO9605119.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            53970..54758
                     /gene="phoH"
                     /locus_tag="ACHLIF_001065"
     CDS             53970..54758
                     /gene="phoH"
                     /locus_tag="ACHLIF_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO9605120.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            55339..56025
                     /locus_tag="ACHLIF_001066"
     CDS             55339..56025
                     /locus_tag="ACHLIF_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO9605121.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
BASE COUNT        13464 a        14173 c        14807 g        13742 t
ORIGIN      
        1 aggcgtttgg ccacatcagg tagaggccga tgtaaagcgt ttttttctat acttgactga
       61 gtataagatg tggaacgttg aagtcctgat tccgtataag aattttttac cttaaaaata
      121 gttgttgcac tggcaggact gttgctggga gaaagaggtg agctggtttt taccgtgtta
      181 ggtgtgcttt tgtgctgtga ggcagtgaag ctactgttgc gcgtaacact tgatatactg
      241 tgaggaatgc tggaaaaaaa agaattaatg ctatttatca tatagataac cttataagaa
      301 ctttatgtga tagtctaatt tatccgtttg catgaaatct accttttgtg aaattctcgt
      361 caatatttta ctcaaaatga tcggctgtag gaataaacta tttgatatta ttgcgttttt
      421 agagttgttt tcaatcagct atagataggg cttggcgcca gaatggagaa tatttaacga
      481 gaaatagaag ctttgtatca gacgggcttc cctgaataga gaagttaaat aatcaggatg
      541 atgctaatga atatcaggaa caaggtgagt gtcttcatgt tcactgaaac agggtgtcac
      601 aaaaaatatg gctataagat gatcaatgtg atttgaaata tttataggtt tacagggggc
      661 tgggcatgca tatcttactg caatgcagaa tatgtgctgc ttggcgcagc tctttattga
      721 gaatgttcaa tcttttgttt cagtttcgtg aaggatacgg acggtttctt gtgtaatatt
      781 atacatcgcc tgtttatctt tactgcatac tcctgcaagt gttaatgcta ctccagcata
      841 aatttctgga taactatgtg taaatatatc cagtgagttt acatgtttct ctttcattcc
      901 tgcatcacaa cgaaccttat ttatacctgt cgccattgat tggggagggc tagagccttc
      961 tgcacgaaaa tagtgatgtg acagatctga gatagctttg tatccttctg cctggttcag
     1021 gcctggagca gtccatgttt tacaaaaata ctcggcagcg taacgtgttg caagagcggc
     1081 atcagacgag gaacctgagg ttccgctcat gaccgataat ctcatccgaa taaaaatata
     1141 ccaattggta taccaagttt ataccaagag ccaaaaaaca aaggggctac catttggtaa
     1201 ccccttgatt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     1261 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     1321 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     1381 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     1441 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gccaaaagaa aaataggtcc
     1501 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa aagtttcact
     1561 acgctttatt aacaatactt tctggcgacg tgtgccagtg cagaaggatg agctttcgtt
     1621 ttcagcatct cacgtgaagc gatggtttgc cttgctacag ggacgtcgct tgccgaccat
     1681 aagcgcccgg tgtcctgccg gtgtcgcaag gagaagagac gtgcgatatg aataacgagg
     1741 aaacatttta ccaggccatg cggcgtcagg gcgttacccg gcgcagcttt ctcaaatatt
     1801 gtagtctggc tgccacgtcg ctgggattag gcgcgggaat ggcaccaaag attgcctggg
     1861 cgctggagaa caaaccccgc attccggtgg tatggatcca cggtctggaa tgcacctgct
     1921 gtaccgaatc ttttatccgc tccgctcacc cactggcgaa ggacgtcatc ctttccctga
     1981 tttccctcga ttatgacgat actttgatgg ctgccgccgg aacccaggcg gaagaagtct
     2041 tcgaagacat catcacgcaa tacaatggca aatatatcct cgcagtagaa ggtaatccgc
     2101 cgctgggcga gcaggggatg ttctgtatca gcagcggtcg accgtttatt gagaaactca
     2161 aacgtgccgc tgccggagcc agcgcgatta tcgcctgggg aacctgcgcg tcctggggct
     2221 gcgtgcaggc cgcgcggccc aatccgacgc aggcaacgcc tatcgacaaa gtcatcaccg
     2281 acaaacccat tatcaaagta cctggctgcc cgccgatccc ggatgtgatg agcgccatca
     2341 ttacttacat ggtgaccttt gatcgcttgc cagatgtcga cagaatgggc cgtccgctga
     2401 tgttctatgg tcagcgaatc cacgataaat gctatcgccg cgcccacttc gacgccggag
     2461 agttcgtcca gagttgggat gatgacgctg cccgcaaagg ttactgcctg tacaaaatgg
     2521 gctgcaaagg gcctaccacc tataacgcct gttcttccac acgctggaat gatggcgttt
     2581 ctttcccaat ccagtctggt cacggctgcc tgggctgtgc ggaaaatggt ttctgggatc
     2641 gcggttcgtt ctacagccgc gtggtcgata ttccgcaaat gggtactcat tccaccgccg
     2701 ataccgtcgg tttaaccgcg cttggcgtgg tggcagcggc tgttggtgtg cacgcagtcg
     2761 ccagcgccgt tgaccagcgc agacgtcata accagcaacc tacagaaacc gaacatcagc
     2821 caggcaatga ggataaacag gcatgagcac tcagtacgaa actcagggat acaccatcaa
     2881 taatgccgga cgccgcctgg tggtcgaccc gattacgcgc atcgaaggcc acatgcgctg
     2941 cgaagtgaat attaacgatc agaatgtgat caccaatgcc gtctcctgcg gcaccatgtt
     3001 tcgcgggctg gagatcatcc tacaagggcg cgacccgcgc gatgcgtggg cgttcgttga
     3061 acgtatctgc ggcgtctgta ctggcgtaca cgccctggct tcggtttacg ccatcgaaga
     3121 tgctatcggt attaaagtgc cggacaacgc aaatatcatc cgcaacatta tgctggcaac
     3181 gctctggtgc cacgatcatc tggtgcactt ctatcagctt gccgggatgg actggatcga
     3241 tgtgttagat gcgctgaaag ccgacccgcg gaaaacctcc gaactggcgc aaagtctctc
     3301 ctcttggccg aaatcatccc ctggctattt cttcgacgta caaaaccgcc tgaaaaaatt
     3361 tgttgaaggc gggcagttgg ggatcttccg caatggctac tgggggcacc cgcagtacaa
     3421 actgccgcca gaagctaacc tgatgggctt tgcccactat ctcgaagctc tcgatttcca
     3481 gcgtgaaatt gtcaaaatcc acgcggtctt tggcggtaaa aacccgcatc caaactggat
     3541 tgtcggcggg atgccttgcg ccatcaacat tgacgaaagc ggcgcggtcg gggcagtcaa
     3601 tatggaacgc ctgaacctgg tgcagtcaat tatcacccgc acggcggact tcattaacaa
     3661 cgtgatgatc cccgacgcct tagccatcgg tcagttcaac aaaccgtgga gcgaaatcgg
     3721 cactggtctt tctgataaat gcgttctcag ctacggcgca ttcccggata ttgccaacga
     3781 ctttggcgag aaaagtctgc tgatgcctgg cggcgcggtg attaacggcg acttcaacaa
     3841 tgtgctgcca gtggatttgg ttgatccgca gcaggtgcag gagtttgtcg accacgcctg
     3901 gtatcgatat cccaacgatc aggtcgggcg tcatccgttc gatggcatca ccgacccgtg
     3961 gtacaacccc ggcgatgtca aaggcagcga taccaacatt cagcagctga atgaacagga
     4021 acgctactcg tggatcaaag cgccacgctg gcgcggtaac gcgatggaag tggggccgct
     4081 ggcgcgcacg ttaatcgctt atcacaaagg cgatgctgcg accgttgagt cggtcgatcg
     4141 catgatgtcg gcgttgaacc tgccgctttc cggtatccag tcaacgttag gccgcatttt
     4201 gtgccgcgcg cacgaagcgc agtgggccgc aggtaagttg cagtatttct tcgacaagct
     4261 gatgaccaac ctgaaaaacg gcaatctcgc cactgcttcc acggaaaaat gggaacctgc
     4321 aacctggccg acagagtgcc gtggtgtcgg ttttaccgaa gcgccgcgcg gggcgttagg
     4381 ccactgggcc gccattcgcg atggcaagat tgatctctac cagtgcgtgg tgccgaccac
     4441 ctggaacgcc agcccgcgcg atcccaaagg gcagattggc gcttatgaag cggcgctgat
     4501 gaacaccaaa atggcgatcc ccgagcaacc gctggagatc ctgcgtactc tgcacagctt
     4561 tgacccgtgc ctcgcctgtt caacacacgt gctgggcgac gacggtagcg agctgatctc
     4621 tgtgcaggtg cgttaacagc gaaggagaat catcatgcaa cagaaaagcg acaacgttgt
     4681 cagccactat gtctttgaag cgccagtccg catctggcac tggttgacgg tgttatgcat
     4741 ggcggtgttg atggtcaccg ggtactttat cggcaagccg ctaccttccg tcagcggcga
     4801 ggcgacgtat ctgttctata tgggctacat caggttaatt cacttcagcg ccgggatggt
     4861 ttttaccgtg gttttgctga tgcggatcta ctgggctttt gttggcaatc gatactcccg
     4921 cgagctgttt atcgtgccgg tatggcgtaa aagctggtgg cagggcgtgt ggtatgaaat
     4981 ccgctggtat ctgtttctgg caaaacgtcc gagtgccgat ataggccata atcccatcgc
     5041 ccaggcggcg atgttcggct atttcctgat gtcggtcttt atgatcatca ctggttttgc
     5101 gctgtatagc gaacacagcc agtacgctat ttttgcgccg ttccgttatg tggtggaatt
     5161 tttctactgg atgggtggca actcaatgga cattcacagc tggcatcggc tggggatgtg
     5221 gctgattggc gcgtttgtga tcggtcatgt ctacatggcg ctgcgtgaag acatcatgtc
     5281 cgacgacacg gtgatctcca ccatggtcaa cggctaccgt agccacaaat ttggcaaaat
     5341 aagtaacaag gagcgttcat gagcgagcaa cgcgtggtgg tcatggggct gggcaacctg
     5401 ctgtgggccg atgaaggctt cggcgtgcgg gtggcggaac ggctgtatgc ccattaccac
     5461 tggcccgagt atgtggagat tgtcgatggc ggtactcagg gactgaactt gctggggtat
     5521 gtcgaaagcg ccagccatct gttgattctc gatgccattg actacgggct ggaacctgga
     5581 acgctgcgaa cctatgccgg agaacgcatt ccggcttatc tcagcgcgaa gaaaatgagc
     5641 ctgcatcaga acagtttctc cgaagtgttg gcgctggcgg atatccgcgg acatctgcca
     5701 gcacatattg ccctcgtcgg tctgcaaccc gcaatgctcg acgactacgg cggtagcctg
     5761 agcgaactgg cacgggagca actgcccgct gcggaacagg cggcgctggc gcagctggct
     5821 gcgtggggaa ttgtgccgca accggctaat gaatcgcgct gtctcaatta tgactgtctg
     5881 tcgatggaaa attacgaagg cgttcgcttg cgccagtacc ggatgacaca ggaggagcag
     5941 ggatgagcaa cgacacgcca tttgatgcgt tgtggcaacg aatgctggcg cgcggctgga
     6001 cgccagtcag tgaatcccgt cttgacgact ggcttacgca agcgccagac ggcgtgctgt
     6061 tattaagcag tgacccgaaa cgcacgccag aggtcagcga taatccggta atgattggcg
     6121 aattactgcg cgagtttccc gactatacat ggcaggtggc gattgctgac cttgagcaga
     6181 gcgaagccat cggcgatcgc tttggcgtct ttcgctttcc tgccacttta gtgtttaccg
     6241 gcggaaacta tcgcggcgtg ctgaatggta ttcacccgtg ggcggaactg ataaacctga
     6301 tgcgcgggct tgtcgaaccg cagcaggagc gtgcctcatg agcgaaacat ttttccatct
     6361 gctggggcca ggaacgcaac cgaacgatga cagtttcagc atgaatccac tgccgatcac
     6421 ctgtcaggtg aatgatgaac cgagtatggc ggccctggag caatgtgctc acagcccgca
     6481 ggtgattgcg ctgttaaacg agttacaaca tcaactaagc gaacgccaac cgccgttggg
     6541 cgaggtgctg gcagtcgatc tgttaaatct caacgccgac gatcgtcact ttatcaatac
     6601 gcttctcggg gaaggggaag tgtcagtgcg cattcagcag gctgacgaca gtgaaagtga
     6661 aatacaggag gcgatcttct gcggattatg gcgggtgcgc agacgtcgcg gcgaaaagtt
     6721 gctggaggac aaactggagg ctggctgcgc gccgctggcg ttgtggcagg cggcaacgca
     6781 aaatctcttg ccgacagatt cgctgttacc gccgcccatt gatggcctga tgaatggcct
     6841 accgttggcg catgagttac tggcacatgt acgtaacccc gacgcgcagc cgcacagcat
     6901 taatctgacg caattaccca tcagcgaggc tgatcggctt tttctctcac gtctctgtgg
     6961 gccgggaaat attcagattc gtaccattgg ctatggcgag agctatatca acgccacggg
     7021 gttacgccat gtctggcatt tacgctgtac ggacacctta aaaggcccgt tactggaaag
     7081 ttatgaaatc tgcccaatac cggaagtggt gctggcagcg ccagaagatt tggtcgactc
     7141 tgcgcagcgg cttagcgagg tatgtcagtg gctggcggaa gctgcaccga cgtaaaaaga
     7201 cggtaagtat cgctttcagt cttatgaata tcgcaatcgg cgaatacctc tggtcgtaga
     7261 gtttcaggat aaagagggag atctaccatt atcgggttat ttttctctct tcgcctacag
     7321 gagtgcgcat gtgggatgtc attgatttat cgcgctggca gtttgctctg acctgcgctg
     7381 tatcactttt tatttgtacc ccttaccctg gggctgattt ttttgctggc tatcatggaa
     7441 accatttacg tggtcaccgg caaaacaatc taccgcgata tgacgcgctt ctggggtaag
     7501 ctcttcggta tcaattttgc tcttggcgtg gctaccggcc tgaccatgga gtttcagttt
     7561 ggtactaact ggtcattcta ttccaactat gtgggcgata tttttggcgc accactggcg
     7621 atggaagcat taatggcctt cttcctcgaa tccacctttg tcgggctgtt cttcttcggc
     7681 tggcaacggc tgaataaata ccagcacctg ctggtgacgt ggctggtggc gttcggttca
     7741 aatctctctg cgttgtggat attgaatgcc aacggttgga tgcaataccc gaccggtgcg
     7801 cattttgata tcgacaccct gcgtatggag atgaccagct tcagcgagct ggtctttaac
     7861 ccggtcagcc aggtgaaatt tgtgcacacc gtaatggcgg gctacgtgac cggggccatg
     7921 tttattatgg cgatcagcgc ctggtattta ctgcgcggac gggagcgcga tgtcgcatta
     7981 cgttcgtttg ccatcggttc cgtcttcggt actctggcga ttatcggtac cctgcaactc
     8041 ggagacagtt ctgcgtatga agtcgcgcaa gtacaaccgg taaaactggc ggcgatggaa
     8101 ggggagtggc aaacggaacc tgcacctgca ccgttccatg tggttgcctg gccggaacag
     8161 gatcaagagc gtaacgcctt tgccatcaaa attcccgcgc tgctagggat cctcgccact
     8221 cactcattag ataaacccgt gccgggtctg aagaatttga tggctgaaac ctacccacgc
     8281 ttgcaacgcg gacgtatggc ctggctgtta atgcaggaaa tatcgcaagg caatcgtgag
     8341 ccgcatgtgt tgcaggcatt ccgggaactg gaaggtgacc tgggctacgg catgttgctc
     8401 tcccgctatg cgccggatat gaatcatgtc acagccgcac agtaccaggc ggcgatgcgt
     8461 ggcgcgatac ctcaggttgc gccggtattc tggagtttcc gcatcatggt gggctgtggt
     8521 tccctgctgc tactggtgat gctgattgcg cttgtccaga cgctgcgtgg caaaatcgac
     8581 cagcatcgct gggtgctgaa aatggcgctc tggagtttgc cgttgccgtg gattgcgatt
     8641 gaagccgggt ggtttatgac cgagtttggt cgtcagccgt gggcgataca ggacatctta
     8701 ccgacatact ccgcgcactc cgctttaacc acaggacaac tggctttctc actgatcatg
     8761 atcgtagggc tttacaccct gttcttaatc gccgaagtct acctgatgca gaaatatgcc
     8821 cgtctggggc cgagcgcgat gcagagtgaa caaccgacgc agcaacaggg gtaaaggaga
     8881 aaatcatgtt tgattatgaa acattgcgct tcatctggtg gctgctgatt ggcgtgatcc
     8941 tggtggtctt tatgatctcc gacggatttg acatggggat cggctgtctg ctgccgctgg
     9001 tggcgcgtaa tgatgatgaa cgccggatag tgataaacag cgttggtgca cactgggaag
     9061 gcaaccaggt ctggttgatc ctcgctggtg gggcattatt tgccgcctgg cccagagtgt
     9121 atgcagcggc gttttccggc ttttatgtgg cgatgatcct ggtgctgtgc tcactgttct
     9181 tccgcccgct ggcctttgat tatcgcggaa aaatcgccga tgcacgctgg cgtaaaatgt
     9241 gggacgccgg tctggtcatc ggcagtctgg tgccgccggt agtcttcggt atcgccttcg
     9301 gcaacttgtt gctcggcgtg ccgtttgcct tcacaccgca attacgcgtg gagtatctcg
     9361 gcagcttctg gcaactgctg acgccattcc ctttattgtg cggattgctc agccttggga
     9421 tggtgatttt gcaaggtggc gtctggttac aactgaaaac tgttggtgtg attcatctgc
     9481 gttcacagct ggccaccaaa cgcgctgcac tgttggtgat gctgtgcttt ttgctggcgg
     9541 gttactggct gtgggtcggt attgatggct ttgtactgct cgcccaggat gctaacggtc
     9601 cttccaatcc gttaatgaaa ctggtggcag tgctacctag tgcctggatg aataatttcg
     9661 tcgaatcgcc cgttttgtgg atcttcccgc tgctggggtt cttctgccca ttgctgacgg
     9721 tgatggcgat ttatcgtggt cgcccgggtt ggggattttt aatggcgtca ttgatgcaat
     9781 ttggcgtgat tttcacggca ggcatcacgc tgttcccctt tgtcatgccg tcaagcgtga
     9841 gtccaatctc cagcctgacg ttgtgggaca gcacttccag tcagctgacg ctgagcatta
     9901 tgttggtaat cgtgctgata tttttgccca ttgtgttgct ctacactctc tggagctact
     9961 acaaaatgtg gggacgcatg acaacagaaa ctctccgccg taacgaaaac gagttgtact
    10021 aaggagcagg aacaatgtgg tatttacttt ggttcgtcgg cattttgttg atgtgttcgc
    10081 tctccaccct tgtgttggta tggctggacc cgcgtctgaa aagttaacga acgtaggcct
    10141 gatgcggcgc attagcatcg catcaggcaa tcaataatgt cagatatgaa aagcggaaac
    10201 atatcgatga aagcgatctt aatcccattt ttatctcttc tgattccgtt aaccccgcaa
    10261 tctgcattcg ctcagagtga gccggagctg aagctggaaa gtgtggtgat tgtcagtcgt
    10321 catggtgtgc gtgctccaac caaggccacg caactgatgc aggatgtcac cccagacgca
    10381 tggccaacct ggccggtaaa actgggttgg ctgacaccgc gcggtggtga gctaatcgcc
    10441 tatctcggac attaccaacg ccagcgtctg gtagccgacg gattgctggc gaaaaagggc
    10501 tgcccgcagt ctggtcaggt cgcgattatt gctgatgtcg acgagcgtac ccgtaaaaca
    10561 ggcgaagcct tcgccgccgg gctggcacct gactgtgcaa taaccgtaca tacccaggca
    10621 gatacgtcca gtcccgatcc gttatttaat cctctaaaaa ctggcgtttg ccaactggat
    10681 aatgcgaacg tgactgacgc gatcctcagc agggcaggag ggtcaattgc tgactttacc
    10741 gggcatcggc aaacggcgtt tcgcgaactg gaacgggtgc ttaattttcc gcaatcaaac
    10801 ttgtgcctta aacgtgagaa acaggacgaa agctgttcat taacgcaggc attaccatcg
    10861 gaactcaagg tgagcgccga caatgtctca ttaaccggtg cggtaagcct cgcatcaatg
    10921 ctgacggaga tatttctcct gcaacaagca cagggaatgc cggagccggg gtggggaagg
    10981 atcaccgatt cacaccagtg gaacaccttg ctaagtttgc ataacgcgca attttatttg
    11041 ctacaacgca cgccagaggt tgcccgcagc cgcgccaccc cgttattaga tttgatcaag
    11101 acagcgttga cgccccatcc accgcaaaaa caggcgtatg gtgtgacatt acccacttca
    11161 gtgctgttta tcgccggaca cgatactaat ctggcaaatc tcggcggcgc actggagctc
    11221 aactggacgc ttcccggtca gccggataac acgccgccag gtggtgaact ggtgtttgaa
    11281 cgctggcgtc ggctaagcga taacagccag tggattcagg tttcgctggt cttccagact
    11341 ttacagcaga tgcgtgataa aacgccgctg tcattaaata cgccgcccgg agaggtgaaa
    11401 ctgaccctgg caggatgtga agagcgaaat gcgcagggca tgtgttcgtt ggcaggtttt
    11461 acgcaaatcg tgaatgaagc acgcataccg gcgtgcagtt tgtaatgcat aaaaaagagc
    11521 attcagttac ctgaatgctc tgaggctgat gacaaacgaa gaactgtcta atgcgtagac
    11581 cggaaaaggc gttcacgccg catccggcca ctttcagttt tactctttct cggagtaact
    11641 ataaccgtaa tagttatagc cgtaactgta agcggtgctg gcgcgtttaa tcacaccatt
    11701 gaggatagcg cctttaatat tgacgcctgc ctgttccaga cgctgcattg acaaactcac
    11761 ctctttggcg gtgttcaagc caaaacgcgc aaccagcagg ctggtgccaa cagaacgccc
    11821 cacgaccgcg gcatcactca ccgccagcat cggcggcgta tcgacaatca ccagatcgta
    11881 atggtcgttc gcccattcca gtaattgacg catccgatcg cgcatcagca gttcagacgg
    11941 gttaggtggc acctgaccgc gagtaatcac atcaaagcct cctttgccaa aatgctggat
    12001 cactttgttg agctcatctt tacctgccag atattccgac aagccatgtt cattactcac
    12061 ggtaaacagg ttatgcgaat aaccacggcg taagtcggca tcaataaata acactttttg
    12121 atcggactgg gcgatcaccg ctgccagagt tgaactgaca aacgttttac cactgtctgg
    12181 cgtcgcaccg gtgatcatca gaatgttatt ctccgtctcc atcatagcga aatgcagact
    12241 ggttcgtagc gcacgtacgg cttccacagc agaatccgcc gggttatcca ccgccaggaa
    12301 ggggatattt ttagtacgat ggcgctgctg attagaaaat aaatttttct tacgcagacg
    12361 ggtgcgttta tccagccact cggacattgg gatagtggca taaacgctga tgccgtgctc
    12421 ttccagttgt tccggggctt ctacaccacg acgcaacatc gcacgcgcca gcacggcacc
    12481 cacagaaata aacaggccaa gaataaaacc aagcaccaca ttcaacgctt ttttcggttt
    12541 cactggctgc ggctgagtga ctgccgggtc gataatccgc acgttaccaa tggcactgga
    12601 tttcgaaata ctcaactcct gctggcggtt aagtaattgc agatataccg cacggcccgc
    12661 ttctacgtca cgacttaaac gcaacacttc ctgttgggtg gaaggcattg ccgatacccg
    12721 cttattcagg cgtttgcgtt cttgctccag cgtctggcgt ttttccagca gcgcacgata
    12781 agttgggtga tctttcttat acagctggga gatctctgcc tcgcggaagg tcagctcatt
    12841 gagttgatta tcaacgttca caatctgctc aagaacggct ttggcttcca ggttaaggtc
    12901 aaccgaatcg cgctgctggc gataaacgtt gagtttttct tccgcctggt ccagctcgct
    12961 gcgcacttca ggtaactggc gctgtaagaa ttcaaggctt tgtgaatcct gcgccgcctg
    13021 gcgagcgata ttctgttgca aatagttgtt agcgatgctg ttcagaatac gagtaatcaa
    13081 ctggggatca tcaccagtca tggtaagttc cagcatcccg ctttctttac tgcgttcgct
    13141 aacggtaaag gtttcctgca atgcgttaat cgcttccagt tcggtacgct ggctcaggac
    13201 aaactgtgtt cctggtttgg ccttaatgtc cgcgatagtc agcgcaacgc catctttttc
    13261 cagacgctgg ccgaccatac cattgacggt gaactcttca ccttccagtg tatagtggcc
    13321 gttttcccca accgtgagtg tcagttgctg atcctgacca ttcagttgtg gaatatgcat
    13381 ccagctgatc gccagctcac ctggtttttc tttggttaat ctcgcccagc cgcgacccac
    13441 aatcggaaaa tacttctgct caactatgtc gcgcagattc agttcagcaa tggttttacc
    13501 gagaatcatg cgcgattgca gcagttggat ctccggtgca gactcgggcg atgagttagg
    13561 gatcatatcg ctcaggccgc tgagaatggc gttgccctgt ttttgctcaa cctggaccag
    13621 agtatctgcc tgataaattg gtgtgcttaa cagcgagtaa gcgacagcga tcagcgtgaa
    13681 taacgcggtc acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag
    13741 cagatcgatc tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat
    13801 cgtattttat aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct
    13861 ccaacatacc gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct
    13921 ctttctgttc cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg
    13981 cggtaacctg agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca
    14041 tctcagcagt gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa
    14101 catctgccgc tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga
    14161 cttttacgcc cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt
    14221 tgccggtaca aaccaccagg attgagttaa attttagttg ggccatgttt gaatccaccg
    14281 tgaagtctct gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg
    14341 gttccaacga gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa
    14401 ttccgttccg agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat
    14461 tttgccattc tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac
    14521 atcctgattc ataccctcag cgttacccag cgcttccgcc agggtcatac cgctgcggtc
    14581 cattttcagc gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt
    14641 gcgcggaata aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg
    14701 ctgcatcagc gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt
    14761 gcgccagtct gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag
    14821 aggaatattg gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt
    14881 ttgcgagcgg aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatccag
    14941 acgcgctgta atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc
    15001 aatatagggg tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct
    15061 acggtattgc cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac
    15121 atccccaatg ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc
    15181 ctgcgacata atcggtttgg cgcgcaactg atcaatcaat cctggcgtga cgggatagat
    15241 attgaccatt ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact
    15301 ggtcgataaa ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt
    15361 cagtaccgaa aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat
    15421 gttatgaatt acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggata
    15481 gagctggtat ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac
    15541 caccgcgcgg ttgcggttag gtccaatggt catcaagtcg aatggaatcg agatataaaa
    15601 acctttacta aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca
    15661 tacccctacc gccacgccgc tgtcaaagcg tttggcgacg tcgatcgttg cgcctttatc
    15721 ttttgccaga tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt
    15781 ccagtaagcc gtcacgaaac ctgttggcgt ggaataatcg gtgaaacgca tcatgttgtc
    15841 ccagtcacgt tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg
    15901 gcgataaagc agctcggaac cgacaccggc gtacatcgtt tccagataac cgccatacac
    15961 ctggccatag aaaccattgc ctaagtcggc aaagtagttc gcctgcaagt tgttgagata
    16021 aacgtcattg cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc
    16081 gggcaacagc gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgcc
    16141 atcaagcagc aggtggtcgg taaaccagta gcgggcactg gacatcagcc ccagctggaa
    16201 catatagaaa tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct
    16261 aaagcgatct tcacgaatac ggtagccccg accaaaggca gaaagatctt ctgcttcaac
    16321 acgttgttgt tgaagttgct ctgattgacc aggcgctgta ccagccagct gtttgcgcag
    16381 gctggctacg tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct
    16441 aatcttctca acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc
    16501 ttcgcgagaa tcacggtatt tgtattgttg accagacata tacagcgtct tatcgcgcag
    16561 ctgaatttcc ggtgcttcaa aacctgcgtt gtacttcagc gcggtaagtt ggttcgctac
    16621 tgtggtgtac tgcaatcctt cagattcagg cgcaggttga tatgccggtt ttggcgtatc
    16681 gcgcagggca gggcgcagat cgttgaaatt ggtccgtaac gtgaagccaa acatcaacgt
    16741 gttaccgcgt tcataactca ggttgagatc tgcccagctg gcagcgcgat aaactgcgcc
    16801 gacgttgaaa tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc
    16861 atcgtattcg agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa
    16921 gatggaagcc gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc
    16981 tgcgcgatga caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt
    17041 accggcgtag ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc
    17101 caccagatac tcaccgtcaa acagacccgt accagcaata tcacgtttac caaacgccac
    17161 ttgcggtagc cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt
    17221 gtatgactga tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt
    17281 ataacgaatg gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta
    17341 ttgatcgtta tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg
    17401 catctgcaac aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta
    17461 ggttaatact tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata
    17521 agaattcttc ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt
    17581 aaatcagcat atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca
    17641 ggcggtggtt ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc
    17701 tcaggcgtaa tcaccataac gttattcttg tccgcgcctg caaggcgggg gtgatcttgc
    17761 agataatcgg taacgctacg gcctgcctgc cagggcaatt gcccggcccc agaaaccgca
    17821 cccagcagag taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac
    17881 gggggattgc tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga
    17941 ccagtgatat tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg
    18001 tcgtcagctt ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc
    18061 gctttagctt tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc
    18121 agttgcggtt gtgtcaccaa ctggacaaca ttttccatgg ggccaacgga aagtgtctgt
    18181 tgttcgccag gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg
    18241 taaagtacgc tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg
    18301 tcatttctac gggaataacc cctgcgccta acatttgccg actttgacga acctggccag
    18361 tagccgaatc gacccagaat gtgttttgcc actgtttttc tggccgcgtg gaagtcacct
    18421 cttcctgcca gacattacac agcacattgc gacccgcaat attcagcgtc tctaatccag
    18481 caaaggaaaa agtagaactc agggtcgcag agcgaaaatg gttgtcttca ctccagagaa
    18541 tatcgcgcgt ccagcggctg ccatctttta ttgccagcgc gttacgcaga gggtcctgcc
    18601 cggaatttgt cacttccagc agattattat taagtttgac ggtttttaaa agacgtccat
    18661 tgtgggtaac cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat
    18721 atcccagtac cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag
    18781 gcaacgcctg gatctgctga tccgctacgg tgatatcctg attatcgaaa aggctggcgc
    18841 gaaatgtatc gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa
    18901 tcgataaaat aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc
    18961 tctaaagccc ggtacgaata tcgcaaccgg gtatcattta actttaattt cgacgcgtta
    19021 aagcagtatt agcgtgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca
    19081 ctgtcatttg ctgcaacaac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca
    19141 actgtcgccg tgccgacagc gccagcagcc acgccaccag tggtagtagc agtcgtggtt
    19201 gcgccggtag tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgtt
    19261 gcttcagggg cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa
    19321 agcttgtgtt tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc
    19381 aggattgtct actttgagta taacatcctt gtgaaatctc cctttcctca taccgcttgc
    19441 actatatact gcgggtagga aaggcgcgca gagggaaata taagattgtt aactatcccc
    19501 ctctggaaat acgacttgtg agcataagat aaatcttagc aaaatacaaa ttaaagataa
    19561 ggaaattgtt acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactatag
    19621 ctggctgtta aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag
    19681 tttctttgtg gaaaagtaac tggtgttata atgtatgatg ttgactattg cactattcca
    19741 ataaaaccag tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct
    19801 gttggtcctc gcaggccatt tacgcggcaa aattccacac gtaatcctgg tataagcact
    19861 tctgcgtcgc ggggagtgaa tgcggaaata tggacctgaa cttctttacg accgtcggag
    19921 gggataatga atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta
    19981 cgggacaaac aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata
    20041 aagccagcga tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat
    20101 catgcgttaa tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag
    20161 cagttctcat tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtag
    20221 tttctgtatt aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta
    20281 ataaaatgac tggtttagta aaatggttta acgcagataa aggttttggc tttatcactc
    20341 ctgatgatgg cagcaaagac gttttcgtcc atttcactgc catccagagc aatgaattcc
    20401 gcacgctgaa cgaaaatcag aaagttgaat tttctattga gcaggggcaa cgtggccccg
    20461 cggcagcgaa cgttgttacg ctctaaggtt gccgttatta ctcaacatct ccatttccgc
    20521 tgtccatgtt gtcatggttc acagtaccgt acatcggcat tcgatgtgac ggagcgaaac
    20581 cctttgggcg ctaagtgtat tttttgtaaa tcgacgatga tcacctttga taacgtcgcg
    20641 ctgcaaatac gcacggacca tgcgccgctg gatttcacaa aataatatca ggctccctcg
    20701 tggagccttt tttatatctg ccttattttt cttcaacgct gtatgtatag taagcgataa
    20761 cctgttgatt attgaatctt tcggggagat ggcttataac atttcttacc tgaccagggt
    20821 accgggaacc aacaccttac tggcgtgttg ctgtctttca agaccagaag aggttaacag
    20881 tgaatattga agagttaaaa aaacaagccg aaacggaaat cgccgacttt atcgcgcaaa
    20941 aaatcgccga gctgaacaag aatacaggga aagaagtctc tgaaattcgc ttcaccgcac
    21001 gagaaaaaat gaccgggctt gaaagttatg atgtcaaaat caaaataatg tgattttgtg
    21061 aacatcaccc gtgcgaggtg atgttccgct tgttgctaat ttagtgacca atcattggcg
    21121 cttgtggaat taagcgtcgg tacaattcat ccggtaccgg gctttgccat actcccgcat
    21181 acattgcgta accaatcacc gcaaacataa tccccagaac cagtagcgtc attaaccagc
    21241 cagacaacgc aaaggctttt ttatttgccg caggtttttg cagtgaaaag gtcaatgttg
    21301 aggctaccgg acatgactct acgcaagtca tacagccggt acattccact gttcgtacct
    21361 gaattaattt atcgaccggg atccgtgatg ggcaattttt tgcgcatttg ccacagtcga
    21421 tacaactttc ggcattgcga cgaatcttaa acggcgacaa tagcgaaacc acgcccatca
    21481 gcgcgccata tgggcaaaga taacgacacc aggcatggcg aataaacagg ctggcaatca
    21541 gcaaaacggt cacgctgatt aatgtcgcgg tccccatatg acgaaagaaa tcgagcattt
    21601 taacgtccat caccacgctg tagggcgaca acataaaata gtgaatcgcc tgagcgggca
    21661 tcaataatgc gatatagaga aaaaaactca acagcaaata cttcacgccg cgcagaggaa
    21721 tatccagcca gcggggaagg acacattgcc gaccaaacag tttgttaccg agatcgccga
    21781 ttaattcaga aagcgtacca accgggcata accatgagca aaaggccttt ttgagtaata
    21841 gactgatgac gataaaagcg accaataaca gcatcgcggc ggcgtggacg gacggtaact
    21901 gacctgttgt aaggctatat ttcagattca tcagaccggc aatcggtagc cagccttcga
    21961 tacctcccgg tctggcgaca aatgtcgtgc tacttgccgt ttcgtaatag cgcacccaat
    22021 accagaacgt gatggcaata taaatattca ttgccaacag taataattgc gtcgctttac
    22081 gccaggtcgt ggcattgcgc cagtcattcc acggtaattt gccgcccgtc gtgcctggcc
    22141 gccgctgcca gcgggttctt tttttctctg ccatgatttt gccagtccgt taagttgcat
    22201 accaaatgcc actattctag ttgttcttaa ctggctgata ttgattcaaa tcgcgttcag
    22261 gtctttctta ttcaaccatg cttccagatc ggcaacactg cgtgtaattt cttcgtgtgg
    22321 aaggggggca gataatggct gctgctccag ttgtgcgcat agctggctgg cgatacgcat
    22381 tcccaaactc gagcaactgc tttttagctg atgcgcggca cgctttattt tctcgctatc
    22441 ctgactggcg cgggcaatgt cgatttcatc gagaagcggc agggcatgtt gtgtaaataa
    22501 taccagccat tcgtggatct tctccgtccc cattaactga gcatcttcat tgagttgcga
    22561 tacatccagc gattgatcgt tattgacttg cagttggaga tagtgcgcca gtaactgacc
    22621 gagcacttca cgcggcaccg gtttagggat aatcccgcgg aataatgaac tggtacgctg
    22681 gcgcagcgtt tcgtcaatga catgggcgct aaagccaatc aaaaccagcg acggatattg
    22741 ctgtgccagt tgtcgggcaa gcgtaatgcc gtcgacatcc ggcagatcaa aatccaccag
    22801 tgcggcagca aacggttcgc tattttgcag tgtctctaaa gcctgcgcgg cattgccaac
    22861 agcaacaacc tgcgcaccac tggtgttcag catctcaacg gtaattcgct gggttagcgg
    22921 gttatcttca attaacagca aacgtaaacc gtcaagacgc accgcctgat tgactgtttt
    22981 tggcacgggt gccgtggcaa cacgtaacgg caagcgtaaa caaaaacagc ttccaacctc
    23041 cggcgtgctg gtggcgctca gttcgccgcc catcgcctgg gccagacggc tactgatagt
    23101 cagccccagc ccggtgccgc cgcgtttgcc gcttacctgc acaaatggct ggaagatttc
    23161 tgccagtttc gcgggatcaa taccgcagcc gctgtcttcc acttcgacca gccattgctc
    23221 gccatcagtg cgactacgca ggataatgta cccttcgtca gtaaaacgca gggcgttgct
    23281 caacaggttg gttataacct gacgaatacg tcgtggatcg cccattaacg cgcacggcat
    23341 atcatcggca attgccgttg ccaggcgaat cgggcgacct ttcacccgtc cgctcattaa
    23401 ttgcagggta ctttccagca gcgggcgcgg ttcaaagggc tcatcgctga ccgaaacatt
    23461 cttgccacct gcttcgatag cggaataatc gagaatatcg ttgaggatgg tcagcaacga
    23521 ttcaccagaa tcagtaattg cccgcaaatc atcacgctgg gcgttaagtg cggggttatc
    23581 tgccagcagt tgagcagtgc cgagaatacc gtacagcggt gtgcggatct catggctcat
    23641 cgccgccaga aacgccgatt ttgcctggct ggctttttct gcttccgccc gtgcctgtcg
    23701 gtgttctatc accagttcct gcaattcagc tgtacgcgct ttgacctgcg ccgccagctg
    23761 ttcgcggtgg cgattcagtg catgaacatt gctgcgaaac gcatccatca gccgcccgat
    23821 ggtatccagc tcccgtacgc cagcggtttc cgggaaaggg gagtcaatat caccgtccag
    23881 cagccgttgc agcgcctgcg tttgttcggc aagtggacgc gtgactgagc gataaaccac
    23941 gcgccagagg atcagaatca gtgcgcaaag agaaactatt cccagcaata acaggctgta
    24001 ttgcccgcgt gcactcgctt tttccagatg cgccagtccg tgctgattac gcagctcaat
    24061 ggtatcgacc agctgactga cttcgctact aaactgcgcg aactgggcga tgttattttg
    24121 ggcgagagtt tgcaggtgag tgctgatttc actgtcctgc tgatacagcg ccagtagatc
    24181 gctatattgg ctaacggtag ttaacgttgt tgcgacctgc gcacgaactc ccggatcttc
    24241 aatgcgtatt tgccgacgtt gcagaatttt caccgcatta ttgagctgct tttccagcgt
    24301 tggtgcattt ttctggatct gctccagccc cagattcatc accatttgct gcacccgcag
    24361 agcgctaagg cgcagttcat tcatctggtt aacatactca agatcgatat caatcagccg
    24421 atcgagtgca ctttcagcag cctgacgctg atcttgttcg atcaaatcgt aaatcccggc
    24481 ctgggtcgct ccagcggaag ttgtcgcatt attcgcctga ccttgcgcca ggcgtgcgat
    24541 ctcatcggcg gcagcgacta tctgctgact gagttgccgt tgttgctggc gtagttgcag
    24601 acgccgcccc accagttccc cttgctgacg taatgaacgg gagatctcct gctcctgttg
    24661 ttcaatagcg gtggtatcaa aaccttgttc ccgtaacgct tgcagcaacg cattaatctt
    24721 caggctttgt gcggtgagca ttcgcccctg cgcctgccac atcttttcgt tatcggcact
    24781 ggtcaggttc tgcgcggcga aaagttccca ggcgctggct tcgctcaact ggcgcgccat
    24841 attcatggta ggaatcaatg cctgagtgtt gtctttttcc acctggctga taaagcgcag
    24901 gttgtaccat cccaccaggg tactggtcag ggttaacagc gccatcaggg caaagcccat
    24961 ccagagtctt cgggtcaggg ttaaattcac ggtcggtgca cttaaggtga aaaaggttga
    25021 gtcgcaaagc ggaatgcatc tagcataaag ccttattatt gatgaggcta tcatgcgcgt
    25081 actgctattt ttacttcttt cccttttcat gttatcggca ttttcggctg ataacctgtt
    25141 gcgctggcat gatgcgcagc attacacggt gcaagcctct acgccgctta aagccaaacg
    25201 cgcatggaaa ctgtgcgcgc tttatcccag cctgaaagat tcatactggt tatcgttgaa
    25261 ctatggtatg caggaggcag ctcgccgcta tggtgtggat ttaaaagtgc tggaggcagg
    25321 cggctacagc cagttggcta cccagcaagc acaaatcgac cagtgtaaac agtggggcgc
    25381 agaggccatt ttgctcggta gtagcactac gtcatttccc gacctgcaaa agcaggtagc
    25441 aaatctgccg gtgatcgaac tggtaaatgc tcttgatgat cccctggtga aaagccgcgt
    25501 tggtgtaccc tggtttcaga tgggctatca acctgggcga tatttggtgc aatgggcgca
    25561 cggtaaacca ctgaacgtgc tgttgatgcc cggacccgat aatgccgggg gcagtaaaga
    25621 gatggtcgag ggttttcgcg cagccattgc cggaagcccg gtgcgtattg ttgatattgc
    25681 gcttggtgat aacgatattg aaatccagcg taacctgttg caggagatgc tggaacgcca
    25741 cccagaaatc gacgtcgttg ccggaacggc cattgcggca gaggcggcaa tgggtgaagg
    25801 gcgtaacctg aaaacgccgc ttaccgtggt gtcgttttat ctttcacatc aggtgtatcg
    25861 cgggctgaag cggggaagag tgattatggc tgccagcgat caaatggtct ggcaggggga
    25921 actggcggtt gagcaggcca tcaggcaatt acaggggcaa tcggtgtctg ataatgtcag
    25981 cccaccgatt ttagttctga cgccgaaaaa tgccgaccgt gaacatattc gccgctcgct
    26041 gtcaccaggg ggatttcgtc cggtctattt ttatcagcac acatcagcgg ctaagaaata
    26101 accttcacca tgttgcgtca ccagtaaatc cgcgctgagt ttatgacgta aacgacgaat
    26161 taacacatcg acggtgcgca ggtcagggtt ttccacccga cgcgcagaaa gcatacgtag
    26221 cagacgttca cggctgagaa tttcgcccgg attcgtcaca aatgccacca acatttcata
    26281 ctctgcgcgg gtcagtttaa tcggctcgcc atcccgctcc agcgtatggc gcgacacatt
    26341 caggcaataa ccggcaaagc gatagcagtt gtcctgagtg tgcggttgag cttgtcgcgc
    26401 gaggtcgatt cgccagagca gatttttcac ccgtactacc agttcgcgca gttccagcgg
    26461 tttggtgacg taatcgtctg cgcccatttc cagcccaaca atacggtcaa tccgatcgct
    26521 gcgtccggta accagaataa tccccaccgt tgagcgttct cgcagggcgc gggttaacat
    26581 caggccattt tcatcgggta agttgatatc cagcagaatt aaatctaccg gctgattctg
    26641 cataatttcc cgcagcccgg caccgctcgc tgtaacggaa acggtatacc cctcctgagt
    26701 gaagtaagat tgtaatcgcg cctgggtaac cggctcatct tcaacaataa caatgtgatg
    26761 tggcatcaga gggttttact cattctgttc atatctgttc atattctgcc gtaagccgtt
    26821 catcctgacc agtgccgctg ttcatatttg ctcattaaga tcgcttcact aaaccataat
    26881 tttacagggg ctattatgcg gaaactctgg aacgcgctac gccgacccag tgctcgttgg
    26941 tcggtactgg cgctggtcgc aattgggatt gtgattggca ttgcgctgat tgtattgcca
    27001 cacgttggga tcaaagtcac cagcacaacc gaattttgtg tcagttgcca cagtatgcaa
    27061 ccggtgtatg aagaatataa acagtcggtg catttccaga acgcctccgg cgtgcgagct
    27121 gaatgccatg actgtcatat cccgccggat attccaggca tggtgaagcg caaactggaa
    27181 gcgagcaatg atatctacca gacctttatt gctcactcca ttgatacacc tgaaaaattc
    27241 gaagccaaac gcgcggaact tgccgagcgt gaatgggcgc gaatgaaaga aaacaactcg
    27301 gcaacctgcc gctcctgcca taactacgat gcgatggatc atgcgaagca gcatcctgaa
    27361 gcggcacgtc agatgaaggt ggcagcgaaa gataatcaat cctgcatcga ctgtcataaa
    27421 ggtattgccc accagttacc ggatatgagt agcggcttcc gtaagcagtt cgatgagctg
    27481 cgcgccagtg ctaatgacag tggtgacacg ctgtactcta ttgatattaa gccaatttat
    27541 gcggcgaaag gcgataaaga agcctctggt tctctgctgc ctgcttcgga agtgaaagtc
    27601 cttaaacgtg acggcgactg gctacaaatt gaaattaccg gctggacgga aagcgccgga
    27661 cgtcagcgtg tactcaccca attcccaggt aaacgcatct ttgttgcctc gattcgtggt
    27721 gatgtgcagc agcaggtaaa aacgctggag aaaaccaccg ttgccgacac caataccgag
    27781 tggagcaagt tgcaggccac tgcgtggatg aagaaaggcg acatggtgaa cgatatcaaa
    27841 ccgatctggg cttatgcgga ttcgttgtac aacggcacct gtaaccagtg ccacggtgca
    27901 ccggaaatcg cccactttga cgctaacggt tggatcggca cgctcaacgg catgattggc
    27961 tttaccagtc tcgataaacg tgaagaacgc accttgttga aatatctgca aatgaatgcg
    28021 tctgacaccg caggtaaggc tcacggcgat aagaaggaag aaaaataatg aacaataacg
    28081 atctctttca ggcatcacgt cggcgttttc tggcacaact cggcggctta accgtcgccg
    28141 ggatgctggg gccgtcattg ttaacgccgc gccgtgcgac tgcggcgcaa gcggcgactg
    28201 acgctgtcat ctcgaaagag ggcattctta ccgggtcgca ctggggggct atccgcgcga
    28261 cggtgaagga tggtcgcttt gtggcggcaa aaccgttcga actggataaa tatccgtcga
    28321 aaatgattgc cggattgccg gatcacgtac acaacgcggc gcgtattcgt tatccgatgg
    28381 tacgcgtgga ctggctgcgt aagcgccatc tcagcgatac ctcccagcgc ggtgataacc
    28441 gttttgtgcg cgtgagctgg gatgaagccc tcgacatgtt ctatgaagaa ctggaacgcg
    28501 tgcagaaaac tcacgggccg agtgccttgc tgaccgccag tggttggcaa tcgacgggga
    28561 tgttccataa cgcttcgggg atgctggcga aagctattgc cttgcatggt aatagcgttg
    28621 gtacgggcgg agattactct accggtgctg cgcaggtgat cctgccgcgc gtggttggct
    28681 caatggaagt gtatgaacag caaacctcct ggccgctggt attgcagaac agcaaaacca
    28741 ttgtgctgtg gggctctgat ttgctgaaaa accagcaagc gaactggtgg tgcccggatc
    28801 acgatgttta tgaatattac gcgcagctaa aagcgaaagt cgccgccggt gaaattgagg
    28861 tcatcagcat cgatccggtt gtcacatcca cccatgagta tctggggcgc gagcatgtga
    28921 agcacattgc ggttaacccg caaactgatg tgccgctgca actggcgctg gcgtatacgc
    28981 tgtacagtga aaacctgtac gacaaaaact tcctcgctaa ctactgtgtg ggttttgagc
    29041 agttcctgcc gtacctgctg ggtgagaaag acggtcagcc gaaagatgcc gcatgggctg
    29101 aaaaactgac cggcattgat gccgaaacca ttcgtgggct ggcgcggcag atggcggcga
    29161 acagaacgca aattattgct ggctggtgcg tgcagcgtat gcagcacggt gaacagtggg
    29221 cgtggatgat tgtggttctg gcggcgatgc tggggcaaat tggcctgcca ggtggtggct
    29281 ttggttttgg ctggcactat aacggcgcag gcacgccggg gcgtaaaggc gttattctga
    29341 gtggtttctc cggctctacg tcgattccgc ctgttcacga caacagtgat tacaaaggct
    29401 acagcagcac cattccgatt gcccgtttta tcgatgcgat cctcgaaccg gggaaagtga
    29461 tcaactggaa cggtaaatcg gtaaaactgc cgccgctgaa aatgtgtatt tttgccggaa
    29521 ctaacccatt ccatcgccat cagcagatca accgcattat tgaaggctgg cgcaagctgg
    29581 aaacggttat cgccatagat aaccagtgga cctcaacctg ccgctttgcc gatatcgtgc
    29641 tgcctgcgac cacgcagttt gagcgtaacg atctcgacca gtacggcaac cactccaacc
    29701 gtggcattat cgccatgaaa caggtggtgc cgccgcagtt cgaggcgcgc aacgactttg
    29761 atattttccg cgagctgtgc cgtcgcttta atcgcgaaga agcctttacc gaagggctgg
    29821 acgaaatggg ctggctgaaa cgcatctggc aggaaggtgt acaacaaggc aaaggacgcg
    29881 gcgttcatct gccagcgttt gatgacttct ggaataacaa agagtacgtc gagtttgacc
    29941 atccgcagat gtttgttcgc caccaggcat tccgcgaaga tccggatctc gaaccgctgg
    30001 gcacgccgag tggcctgatt gagatctact cgaaaaccat cgccgatatg aactacgacg
    30061 attgtcaggg gcatccgatg tggtttgaga aaatcgaacg ctcccacggt gggcccggct
    30121 cgcagaagta tccgttgcat ctgcaatctg tgcatccgga tttccgactt cactcgcagt
    30181 tatgtgagtc ggaaactctg cgtcagcaat atacggtagc gggtaaagag ccagtgttca
    30241 ttaacccgca ggatgccagc gcgcgcggta ttcgtaacgg tgatgtggta cgcgtcttta
    30301 acgctcgcgg tcaggtgttg gcaggggcag tagtttctga ccgctatgca cccggcgtgg
    30361 cacgaattca cgaaggggca tggtacgatc cagataaagg cggcgagcct ggtgcgctgt
    30421 gcaaatacgg taaccccaac gtgctgacca tcgacatcgg tacttcgcag ctggcgcagg
    30481 cgaccagtgc gcacactacg ctggtggaaa ttgagaagta caatggagca gtggagcagg
    30541 tgacggcgtt taacggccct gtggagatgg tggcgcagtg cgaatatgtt cccgcgtcgc
    30601 aggtgaaatc atgaccacgc tgacagcaca acagattgcc tgtgtttacg cctggctggc
    30661 gcagttgttc tcccgtgaac tggacgatga acaactgacg caaatcgcca gtgcgcagat
    30721 ggctgaatgg ttttcgttgc tgaaaagcga accgccgctg gctgcggcgg tgaatgagct
    30781 ggaaaactgt attgccacgc tgacagtacg tgacgatgcc cgtctggaac tggccgctga
    30841 cttttgcggc ctgtttctga tgaccgacaa acaagcggcg ctgccgtatg catcggccta
    30901 caaacaggac gagcaagaga ttaaacgctt gttagttgag gcagggatgg aaaccagcgg
    30961 caatttcaac gaaccggcag atcatctggc gatctatctc gaattgctca gccatctgca
    31021 tttttcgctg ggagagggga ccgttcctgc gcgaagaatc gacagtttgc ggcaaaaaac
    31081 actgacggcg ctgtggcaat ggttaccaga gtttgttggg cgttgtcgtc agtatgacag
    31141 ctttggtttt tacgcggcac taagccagtt attgctggtg ttagtggaga gcgaccacca
    31201 aaacagataa cgtcgtttgt gcgcctgaaa agacgcgttt agcgtcgcat caggcattat
    31261 ggggcagttg ccggatgcgt catcacatcc ggcaaacaca catcacggat gagttacaaa
    31321 ccgggaaagt cgctggcgca gcaggcggtt ttcctgcttc aggtgcgcaa tatcatccat
    31381 taacgtcagc gccaccgcga tcccaggcca gtccagagcc agttcatgac gcaggcgtac
    31441 tgcgcgttgc accacgatgg cggcatggtc gtcaaatacc caggttgttt cctggatctc
    31501 acgcggttca accaccccca aaccgacaat ttcattcaac tcctcttcag agatgccggt
    31561 atgcaggcaa aattcggtaa tagtaaaagt caccgtaaca ttagccatta tgctttcccc
    31621 caatctttac gtggatcaaa agacgactgg gcgtctgcca gttgctgcca cagcgcggca
    31681 gtgttttcat ccggtttcgg cggcatcacg attttcagta ccgcatacag atcgccggtc
    31741 tgttttttgc tcaccagacc tttgccttta acgcgcaatc gttgcccggc ctggctgcct
    31801 ggcgggatag tcagcaaaat gctttctttc agtgttggaa cggtgacttt agcacccagc
    31861 gccgcttccc acgggctaac cggcactaca atttccagat catggccgac aatatcgaac
    31921 agcggatgtg gcgcaatatg aatcaccagc cacaaatcgc catttggacc gccgttttcg
    31981 cccggcgtcc cctggccttt cagacggatg cgttgaccat tgccgacgcc cgccgggatc
    32041 ttgacattca gcgttttcgg aatttcctgt tcgatcatgc caaaggcgtt ataaaccggc
    32101 aggttatagc tgatggtacg cttatgctca gtaagcgttt cttcgaggaa taccgccact
    32161 tcgatttcaa tatcgtggcc gcgtgtggcg gggcgttgac ggctctggcg ggcatgctga
    32221 ccgaaaattg acgagaagat atcgtcaaaa tcttcggcgt taaaactctg accgtcgcta
    32281 tgatggaact gacggttaaa ttgcggatcg ttgcgatgtt gccacatctg atcatactca
    32341 gcgcgacgtt gttcatcact taacacttcc caggcttcag cgacctcttt gaagcgggct
    32401 tcggcatccg gttctttgct gacatcagga tggtatttgc gggccagtcg acgataggcg
    32461 gtcttgattg tcttgagatc gtccgtcggt ttcacgccca tgatggcgta ataatccttt
    32521 aattccatag cgttatctcg cgtaaatcaa cacaaattga aggaacccct gtaaggtaac
    32581 tcctataagt gtagggtaat cctcaaaatt tcatatgcca acacagaata tgttattgaa
    32641 atcatcgcgg agaggaggtc gcctcaaggt ggggtgctga acatatttta aacaggtgaa
    32701 aaagggtgag cgatttttga tagtggaacc aggcacttta agtttaactg gggcgtcatt
    32761 atttattaaa ttttataggc gctatatatg agtagtaata tacatggaat tagttgcact
    32821 gcaaataatt atttgaaaca agcctggaac aatataaaaa atgagcacga aaaaaatcaa
    32881 aaatattcaa tcacgctttt tgaaaacaca ctggtgtgtt ttatgcggtt gtataaagaa
    32941 atcagacgtc aaaaagctga agactacatt ccatgcctgg aatgcgattc actagaaaaa
    33001 gaatttgagg aaatgcagaa tgataatgat ttatcattat ttttgagaac attgcgtact
    33061 aatgatacag aaacttattc aggggtttca gaaggtatta catatactat acaatatgtt
    33121 cgagatattg atattgttag agtgtccttg ccgggcagag gttcagagtc tatcacagat
    33181 tttaaaggtt attattggta tggctttatg gaatatattg aaaacattaa tgcgtgtgat
    33241 gatgtttttt ctgagtattg tttagatgat gaaaatatga gtatccagcc agaatggata
    33301 aatacgccgg gaatatctga tttagatact ggtattgatt tgtctggtat atcttttatt
    33361 cagagtgaaa ttaataaaac ttatggatta aaatacgctc cagtagatgg cgatggctat
    33421 tgtctgttaa gggccatact ggttttaaaa gaacatgagt attcatgggc gctgggcagc
    33481 cataagacgc aaaagcaagt atacgaagag tttattaaaa ttgttgataa acaaacgatc
    33541 gaggctcttg ttgatacggc attcaatgat ctcagggaag atgtaaagac attattcggc
    33601 gttaatctac aatctgacaa caaaattcag gggcagggtg gttttttatc atggagtttt
    33661 ttgtctttta aaaaagaatt tattgatagt tgcttgaatg acaaaaaatg catactgcat
    33721 ttacccgagt ttatatttaa tgataataag gcacgccttg ttttagacac cgacccagag
    33781 cagaaggtta atgaggtgaa aaatttcctc actgctcttt cagatagcat ttgctcatta
    33841 tttattgtta atggtaatgt ggcatcaatc tccttaggga atgaatcctt ttcaacagat
    33901 gacgaccttg agtatgggta tttaataaac actggcaatc attatgacgt ttacctccct
    33961 cctgaacttt ttgctcaggc ttacgagtta aacaataagg aaaggaatgc gcaaattgat
    34021 tttttaactc gttatgcaat ttactgacaa aggcatgtgc taaaaaccat tgttattagc
    34081 ctcacttctc ttattattgg tactattgcc tctgtattag taacgccgct gatattctgt
    34141 ttagccacag gtgtaatcat cagcggcgta cgcgaggcag ggagctaatc aggcatagtt
    34201 tgcgtcaaac cttgcctgtt tttaaagatg tatatagaaa aacaggcatt caacaagcca
    34261 ttttgcgaac ctgttcccgg aaaaatgtca tatttctgtc acactcttta gtgattgata
    34321 acaaaagagg tgccaggaat gaacaaaacg ctaatcgccg caactgtggc agggatagtt
    34381 ttactcgctt caaatgctca ggcacaaacc gtaccggaag gctatcagct acagcaagtg
    34441 ctcatgatga gccgccataa cttacgtgcg ccgctggcga acaatggcag tgtgctggag
    34501 cagtctacgc cgaataaatg gcccgaatgg gacgtccccg gtgggcaact caccaccaaa
    34561 ggtggcgtgc tcgaagtgta tatgggccat tacatgcgtg aatggctggc acagcagggg
    34621 atggtgaaat cgggggaatg cccgccgccg gacaccgttt atgcctatgc caatagtctg
    34681 caacgtaccg ttgcgaccgc acagttcttt attaccggcg cattcccggg gtgtgatatt
    34741 cctgtgcatc accaggaaaa aatgggcact atggacccaa cctttaaccc ggtgatcacc
    34801 gatgattccg ccgcattcag tgaacaggcg gtggcggcaa tggagaaaga gctcagcaaa
    34861 ctccagctta ccgacagcta ccaactactg gaaaaaatcg ttaactataa agattcccct
    34921 gcctgtaaag agaaacaaca gtgttcgctg gtggatggca aaaatacctt tagcgccaag
    34981 tatcaacaag aaccaggtgt ttccgggccg ctgaaagtcg gcaactcgct ggtagatgcg
    35041 tttactttgc aatattacga aggttttccg atggatcagg tggcctgggg agaaatcaaa
    35101 tctgaccagc agtggaaggt gttgtcgaag cttaaaaacg gctaccagga cagcctgttt
    35161 acctcaccgg aagtggcgcg caatgttgcg aaaccgctgg tcagttatat cgacaaagct
    35221 ctggtcaccg atcgcaccag cgcaccgaaa attacagtgt tggttgggca cgactccaac
    35281 attgcctctc tgttaacggc gctggatttc aaaccgtatc agttgcatga ccagaacgaa
    35341 cgcacgccga ttggcggcaa aatcgttttc cagcgttggc atgacagcaa agccaatcgc
    35401 gatttgatga aaattgaata tgtgtatcag agtgcggaac agttacgtaa tgccgatgcg
    35461 ttaaccctgc aggcacctgc tcagcgtgtg acgctggaat taagcggttg cccgatagat
    35521 gccaacggtt tctgcccaat ggataagttt gatagcgtgt tgaatgaggc ggtgaaataa
    35581 cagaaaactc ccccgcgaga agtgggggag tcgctggtta aacgttttta cgttcgatgg
    35641 tctgttcgcc ccaaaaaagc gaatctttat cggtcttagc aaaggctttg actaacactt
    35701 catcgctacc ttcttcccaa atcttttccg ccattttttc gtcgtacccg gcgacttcga
    35761 aaatggcctc ggctatttcc ggcgacgtat tgcgcagaga tgcccattca ccgacgtgat
    35821 gagctttcgc ttcttgagtt ggcatgcgta tcctcctgtt gaagattagc cgttaagttt
    35881 aactgccaga cctgcgacat attccccttg ataacgagca atagacagtt cttcctggct
    35941 tggctggcgt gagccgtcac cgcctgcgat ggtggttgcg ccgtacggcg taccgccgcg
    36001 aacctgtgaa acgtcaaata attcctgcgc tgcgtagcca atggggacaa ttaccatgcc
    36061 gtgatgcgca agggtcgtcc aggttgaagt aatagtttgt tcctgaccgc cgccagtacc
    36121 ggtggaacta aagacgctcg ccagttttcc gtatagtgcg ccggaagccc acaggccgcc
    36181 cgtctggtcg aggaaggtac gcatttgacc ggacatgttg ccaaagcggg taggtgtacc
    36241 aaaaataatg gcgtcgtaat cggccagttc ttgcggggtt gcaaccggtg cagtttgcgt
    36301 tttaccgcct gctttttcaa ataattgcgg cggcatggtt tccggtacac gcttaacgac
    36361 aacttctgcg ccatcgactt tgcttgcacc ctcagcgact gcgcgtgcca tcgtttcaat
    36421 atgtccgtac atggaataat aaagcaccag aactttagcc atttctaacc actcctcgtg
    36481 ttatctctat tccgtagcga ttcgctacca cttatttaaa gataagacgt ccttttcaga
    36541 gtgcaaattt cacaaccact tatttgattt ataacaactt tcacaagcac gtaattttgt
    36601 cgcaaaatga cacattttta tctcatcgcg tttttttaat cataagagcg gcttatggat
    36661 aattatttga gatgatatct attctcgcta agaagctgtt gcaggatatt accaaacgcg
    36721 ggtctgcccg cgtcagttca ctaagcttag tcccacgtag cgaaaatatg gcagccgcca
    36781 tatgccgcgt taattctatg caatatgatg tctataccca gacggaggtc agtaatggca
    36841 aaccatcgag gcggttccgg caattttgca gaagaccgcg aaagagcatc agaagcaggt
    36901 aaaaaaggtg gacagcacag cgggggtaat ttcaagaatg acccgcagcg cgcatctgaa
    36961 gcaggtaaaa aaggtggcaa gagcagtcac ggcaaaagcg acaactagcc gggctaatca
    37021 atgacgaatg catttttttc ttaggctcgt caaaaagtca tcaccgccgg taatcccggc
    37081 ggttttctgt ttatggctcc tgatgaacaa cttctggcgg tggaacgtca accaactttc
    37141 tgcttaacaa cgcattgagt aaaatcgcgc caaaggttgc tgtaccaatc cctcccaacg
    37201 taaaaccgcc cagcgtgaga gcaaaatcac ccgcgcccag cactaaggtt actgcgacca
    37261 taatcaaatt accgttctgg cttaaatcga cacggttttg tacccatatc cttgcgcctg
    37321 cgacggcaat cagcccgaac acaacaattg atgcaccacc aatcaccgcg gccggaatgg
    37381 tatgaatcag cgcgccaaat ttcggtgaaa agcccaacag catggcgatg acggcagcag
    37441 cgacaaacac cagcgtcgag tagacttttg tcacggccat tacaccgata ttctcggcgt
    37501 aggtggttac gccgctgcca ccgacagaac cagaaagcat cgtcgccagc ccatcaccga
    37561 cgaatgcccg ccccatatac gggtccatat tgcgtccggt catcccggcg accgccttga
    37621 gatgacctaa gttttccgcc accagaatca ccgccacggg cgcaatcagc atcattgcct
    37681 gactattaaa agcaggagtg gaaaaatgtg gcaaaccgaa ccaggcagca tggctgacga
    37741 gagtaaaatc gacggctttt cccagcccta aaaggttggt catcacgcca tacagcagac
    37801 aggcgacaat taaacctacg agaatcaata accgctggat catgccacgg gtaaacaccg
    37861 ccaccagccc aatacacagc accgtcatta ccgccatcca gctatcaaag gccgaagccg
    37921 atacactttt cactgcgata ggcgctaagt tcaggccaat cgccatcacc accgcacccg
    37981 tcaccaccgg cggcattagt cgttcaatcc agcgtgtacc aattttcatc accaccaggc
    38041 caatgacggt ataaaccagc ccacaggcga taatcccgcc cagcgcaatg ctgatattcg
    38101 ggttaatgcc ctgaccgtta aagcccgtcg cggcaatcac cacgccgaca aaagccgcgc
    38161 tggaaccgag ataactgggg acgcgcccgc cggtaataaa gaaaaacagt aacgtgccga
    38221 tccccgacat taaaatggaa agattgggat ccagccccat cagaatcggc attaacaccg
    38281 tcgcgccaaa catcgccacc gcgtgttgaa cgcccattac tgccgtctga gcaaacggca
    38341 atcgttcata cggcgcgacc acgccgctct ctgtagaggt cgattttaac tgccagtgag
    38401 gaaaaccgaa cattgccatc agctgtctcc tttaggaggt taacaagcag ggcgcatcag
    38461 cgcgtgataa ctgcgatcga accacaccag cccgtagggt gtggcgtgac gatgaatcgc
    38521 ttcgatggcg caaaacagaa tgtcgtgggt gccgacgctc accacctggc tgatacggca
    38581 gtcaaacgaa accagagcct cttccagttg cgggcatccg gtcacacccg tctgccagcg
    38641 ggcggcggca aagcggtgtt ccatgggcgt tttgccgcca aaaagatttg aaagcggctc
    38701 ctgcccggcg ctcagtgtat ttacacacag cgttcgattt tcattgaata ccggccagac
    38761 ggacgcccca cgattcaggc acaccagtaa tgtaggcggc gtgtcggtca cgctgcagac
    38821 cgcgctggcg gtgaacccgg cgcgcccggc tggaccatcc gtggtgatga tattgactgc
    38881 cgcgcccata caggacatcg catcgcgaaa ggtttgttga tcgacaatgt tcatggtttg
    38941 ctccttacaa cagcccgcag gcttcttcaa aggacagacg tggcaggcgc gcaaaaagtt
    39001 tgctgctatc gccatagccg atattaatca gcagattgct cttcagcgtg ctgcccgcaa
    39061 aaaaggcgtc gtcaacgtgt tgacggtcaa aacccgacat cgggccggta tccagtccca
    39121 gcgcacggca ggcgacgatc agataggccg cctgcatgga actgttgcga aacgctgttt
    39181 cttcggcaag ttgtgggctg gaggtaaacc aactgcgggc atcgccgtga ggaaacagtt
    39241 gcggtaaccg ttcataaaat tcactgtccc aggcgacgat agcggtgacg ggcgcggtca
    39301 gggttttttg cagattgccg ctggaaagtg ccgggcgcag acgttctttt ccttctgccg
    39361 tgcggataaa cacgatccgt gccggagaac agttagctga tgtcggcccc catttcatca
    39421 gggcataaat ctcccgtaac gtctcatcgc tgacgggtgt ctcccgccag ccgttgtgag
    39481 tgcgggcatc ggtgaacaag gtgctaagcg cacctgggct aacggcttcg ttcatagcaa
    39541 ttccttacag ggcggcttca cggtgatgta acaggctggc aagcccgttg agtaacagag
    39601 cattaaacgt ttcgggatcg gtcacgttgc aggcgtgtcc gccatagggc atcaccattt
    39661 tctggctatc gggcagggcg gcatgaagct cactggaaca tgctgatggc accagcagat
    39721 catcactggc gcagatgatt tgcaccgggc agcggatgcg atccgcatgg tgactaaagt
    39781 cagcgcgttt gagggcgtta agtcgacgca gtaaattatt tttgccctga aaatgcgcca
    39841 gtgccagcgc gtcttctgcc tccaggcgag gtgcgcgggc cgccatccag tcggcgggat
    39901 agaggaacaa cggctgcgct tccacccatg cctgcgcgcc gccgctatac agtaatcgtt
    39961 cgcgaacctg aaaacagcgg cgcgtatggg cgtttattcg tagccagccg ttaacgctga
    40021 tcagcacagt taccgacgcg ggataatcca gcgccagctg cattcccacc agcgcaccga
    40081 gcgcatggcc gaccaccgcg taatgctcaa tccctgcggc taccagcgcc tgatgcagtt
    40141 ccgctgccat ctgggcgata ctgtaatctt ctgccagcgt gtcgggatta ttgccggtgc
    40201 cgcgctggtc gtaacagact acctgatact cctgctccag caccgccagt tgcggtaacc
    40261 agtaactgcc gctaccgcca agacccgaaa tcaacaccac tacgggcgta tcagcataag
    40321 ggggaggtga gagtgaaagt ttcatcgcgg cctcacttgg cgatatgcgc aattgtggcg
    40381 atttccacca gcgcgtcagg ttttaccagt ccgcactgaa tgcagaatcg cgccggttta
    40441 tcacccggaa aaaactcggc gtagatttcg ttaatcgcgg cgtaattttt ccagtcggta
    40501 ataaagatgc tgttgaaggt cacatccgcc atcgtgccac ccgccgtctc gatcaccttg
    40561 cggataattt ccagaacgtg gcgggtttgc gcctttgggt catcggcaaa cagcacgtta
    40621 ttatgttgat caaaagccag cgtaccggag acatacacca cgccatcagc cagcgtgccg
    40681 ggaacgaagg gggccagcgg tgcgctgctg ccagcgggaa taattacgga ttttggcatc
    40741 gttaaactcc ttaagcgata cgagcaaagg atgtgggaga aagcgcgtcg cagaacgttt
    40801 cgacgtcgct gacccagcca aaaaaggttt cgatattgaa caacgcggct ttctgtgcaa
    40861 attccggccc cgcctggtgt gttgcgtctt ccagcaccac gccgaaatac tccagaaaaa
    40921 agccatcgcg tagcgtcgat tcgacgcaga cgttggtagc gataccggtg aaaaccagat
    40981 ggcgtattcc acggctgcgc aaaatgctgt ccagcggtgt attgaagaaa ccgctgtagc
    41041 gcggcttcgg cagcacaata tcgccaggct gcggcaccag ttcatccacc agttgataat
    41101 cccaggagcc tttcgccagc aatttcccct gcagctgcgg ctgcttacgc atggttttca
    41161 gggcgttcga tttatgaaaa ttcggtgagc cggggccgcc agcttcgaca tactgttcat
    41221 cccagccatt ttgaaaccag atgatcagca tccctgccgc tcgcgctgcg gtcacggcgg
    41281 tttgaatgtt ggcaatgacc gggcgagtgg ttgagacatc aaacccggcg agatctaagt
    41341 agccgcctgg cgtggcataa gcgttttgca tatccaccac gatcagcgca ctttgctgcg
    41401 gatcgaaggt aatggcttcc ggtcgagcgg ttaaggttgt catcatgcca cctcctgagt
    41461 cagcgcaggt agatgggcgc ggcactgcat cagtggttga atgcgctcgc cgaaggtttc
    41521 gattcccgac agaaaatcat cgaaggtcaa cagcacgcct tcggcaccag gcacgcttgc
    41581 gacttcatct aacatgcgcg cgacactggc gtaagaaccg actaacgtcc ccatattgat
    41641 gtttaccgcc gaagtgggat cggccatctg acggacgttg gtatcagtac cggagcgggt
    41701 atctttctga ctttgttcgg ttagccagct taacgcctct tcatccgcgc ccgctttgta
    41761 gtgttcccat ttggcgcgag cggcatcgtc ggtttcatcg gcaatcacca taaacaacac
    41821 ataagaacca acgtcgcgcc cggtttgctc tgcggcctgt ttcatccgcg cagcggtcgg
    41881 ggcgaaagcc gtgggcgtat ttacgccttt gccgaaacag aagttgaaat cggcatactg
    41941 ggcggagaac gccatgccag cgtcgctttg cccggcgcag atcactttca tggggacact
    42001 cggttgcgga ctgacgcgac aatcattcat ggtgaaaaaa tcgcctttaa aatcgctttt
    42061 ccccgtgccc cacaggtcgc gcagcacctg aacatattcg gtgagatagt cgtaacgacg
    42121 ggagaaatag tcatcgccag gccagatacc catctgctca tactcgggct tttgccagcc
    42181 agtcacgagg ttgacgccaa aacgcccgcc agagatggag tcgatggttg cggccatacg
    42241 ggcgacgatt gccggaggta acgttaaggt ggcagcagtg gcgtaaatct gaatgcgcga
    42301 ggtgacggcc gccagccccg ccatcaaggt gaacgactca aggttgtgat cccagaactc
    42361 agttttgccg ccaaagccac gcagtttgat catcgacagg gcgaaatcga aatggtagtg
    42421 ctccgctttt tgcacaatgg ctttattcag ttcaaaggtc ggcatgtact gcggcgcgtg
    42481 ggtcgaaatg agccagccgt tgttgccaat aggtacgaat acgccaattt tcatcatcaa
    42541 cctctcttcg tctcgtaaag tgaaagtcag acggggcgct gcatcctgca tatccttttc
    42601 agccgcgtaa tggcttgttt gcaaagcgga tgccagtttt taaaaagtga atgttattaa
    42661 tgtgttaaca ttgtgttgtc taaaatatct ggtaaaaagt ggactaaacg gtcaaaacag
    42721 ttgcacataa aacatgcatc tgtgcgcgat gagagtgcag aaggtcgagg ccgggcgggg
    42781 gttttgctat cctgttgcca atctacaaga ggggagagcg catgacgcaa ggcgcagtga
    42841 aaacaacggg taaacgttcg cgcgcagtaa gcgcgaagaa aaaagcgatt cttagcgcag
    42901 cactggacac tttttcacaa ttcggttttc acggcacaag gctggagcag atcgcagagt
    42961 tggcgggtgt ttcaaaaacc aatctgctgt attactttcc gtcaaaagag gcgctgtata
    43021 ttgccgtgct gcggcagatt ctcgatatct ggctggcacc gttaaaagcg tttcgtgaag
    43081 atttcgctcc gctggcggcg ataaaagagt acatccgtct gaagctggaa gtctcccgcg
    43141 attatccgca ggcttcgcgc ctgttctgta tggagatgct ggcaggcgcg ccgctgttaa
    43201 tggatgaact gacgggcgat ttgaaggcat taattgatga gaaatcggcg ctgattgccg
    43261 gttgggtcaa aagcggcaaa ctcgcgccga ttgatccgca gcatttgatt ttcatgattt
    43321 gggcttccac tcaacattac gccgatttcg cccctcaggt ggaggcggtg acaggcgcga
    43381 cgttgcgcga tgagatattt ttcaatcaaa cggttgaaaa cgtgcagcgg attattattg
    43441 aggggattcg accacgttaa agatgccgga ggaggttgta acatcctccg gctacctgtt
    43501 taacctatag tcattaaact ggcgttaccg ccagcggcag cggtattcac actcagcgaa
    43561 cgttcgatat acagccgttc cagaaggata ttgctttcgc cacgggcaaa accctgcacc
    43621 gaaacaattg cgccatcccg cgcagcaacg gcttcacaca atgcgcgaag ctgatccgaa
    43681 tcaccgtgga agatcaccgc atcaaagggt tgagcggtta tattttccgc tttcgccagt
    43741 tgaatacgtt cgctgactgc cgatggcaat gcctttacta gctgacgatg cagcgcgtca
    43801 tccggccaca gtacctggct gcccaccgcc agcacggcgg cgagctgagt cagcgcatcc
    43861 tgctcatcat cggcaataca caacacgcgc tcacgcggca gcagcgtcca ggtgttgcgt
    43921 tcacccgtcg gccccggcag caatcgttgt gttcctgcct gcgccagctc gccatattgc
    43981 gtacataacg cctgcaattc tggacgattt gctgcccatt cccgcagtgc atttagcggc
    44041 tgagtcaatg cggctttcaa ctgtgcatcg accggatact ctgcatcctg acgcgcgagc
    44101 gtcactgcca gcgcactttc cgggcgattc gccagcagac ggtagagata gagcggaccg
    44161 cctgctttcg gcccggtacc ggacaaccct tcgccgccga atggctgcac acccaccact
    44221 gcgcccacca tattacggtt aacatacagg ttaccaacat gggccgagcc agtgacctgg
    44281 gcgatggttt catcaatacg cgtatggaca ccaagcgtca gaccatagcc ggaagcgtta
    44341 atctgctcga tcagctctgg tagctggtta cggttgtaac gcaccacatg cagcaccgga
    44401 ccaaagacct ctttttgcaa ttcggcaaag tcatccagtt cgatcagcgt cggggcgaca
    44461 aaggtgccgc tttgccattc acgggcatct tcgctgtttt cccgcaccgc ctggaacacc
    44521 ggacggcctt tgctacgcat ggtctgaata tggcgctcaa tattggcttt cgcttcgcta
    44581 tcaatcaccg gaccgatatc ggtggtcagg cgacccggat tacccatccg gcattcggcc
    44641 attgcgccgc gcagcatttt caacgtgtgg tcggcaatct catcttgcag gcacagcacg
    44701 cgcagcgccg aacaacgctg acccgcactg tcgaacgccg aggccagtac atccacgacg
    44761 acctgttcgg tcagtgctga agaatcgaca atcatcgcgt tcatgccgcc ggtttcagcg
    44821 atgagcggaa tagggcgacc ctgagcgtcc aggcggctgg cgatattgcg ctgcagtaac
    44881 gtagcgactt cggttgaacc ggtaaacatc accccgcgta cgcgatcatc acccgtcagt
    44941 tgcgcgccca cggtttcccc ctgacctggc agcaactgca ccacgcctgg cggtacaccc
    45001 gcttccagca aaatggcgat cccttgcgcg gcaatcagcg gcgtttgttc tgccggtttt
    45061 gccagcacgc tgttacctgc cgccagtgcg gcagcaatct gcccggtgaa aatagccagc
    45121 gggaagttcc acggactgat acacaccaca ggccctaatg gacggtgggt ttcgttagcg
    45181 aaatcatccc gcacctgtcc ggcatagtag tggagaaaat cgaccgcttc gcgcacttcg
    45241 gcaatggcgt tactgaaggt ttttccggct tcacgcacca gaataccaat cagttgctgc
    45301 atctggcttt ccattagcac ggcagcgcga tgcaaaatcg ccgcgcgttc aaccggagga
    45361 gtggcaaacc agattggcgc gttattaacc gcactttcca gcgcctgttc tacctcacgc
    45421 ggcgtggctt cacgcacaaa gcccacaata tctttcggtt ccgcagggtt aataacgggc
    45481 gacatctcac ctgccgctac cggttgttcc agcattggca aggcctgcca tttttgcagt
    45541 gcactattga gcagggcaga agagagcgag gccagccggt gttcgttagc gagatccagc
    45601 cctgccgagt tgtcgcgccc gtgaccgtaa agatcgcgcg gcaggggaat tttcggatgc
    45661 ggtaatccag tttgcccttc ctgttgcgcc agtttttcta cagcagtgac cggatcggcg
    45721 accagttcat ccagtggcaa agaggtgtcg gcaatacggt taacaaacga ggtgttagca
    45781 ccgttttcca gcaggcgacg caccagatac gccaacagcg tttcatgggt accgaccgga
    45841 gcataaatac gacacggacg gttaagtttg ccgtcggcaa ctttcccggt gacctgctca
    45901 tacagtggct cgcccatacc atgcaggcac tggaactcgt actgacccgg atagtagttc
    45961 tgacctgcca gttgataaat cgctgccagc gtatgggcgt tgtgcgtcgc gaactgcggg
    46021 tagattagat tcggcaccgc cagcagcttt ttcgcacagg cgagatatga aacgtcggta
    46081 tacaccttgc gggtataaac cggatagcct tcaaggccgt ccatctgcgc acgcttgatt
    46141 tcactatccc agtacgcgcc tttcaccagg cgaatcatca gacggcgacg gctgcgggtg
    46201 gcgagatcaa tcaggtaatc aatcaccagt gggcagcgtt tttgataagc ctgaataaca
    46261 aaaccgatac cgttccagcc tgccagctca ggctcgaaac agagtttttc cagcagatcg
    46321 agggagatct ccagacgatc ggcctcttcg gcgtcaatgt tgataccaat atcgtactga
    46381 cgcgccagca gggtgagtga tttcagacgt gggtaaagct cttccattac ccggtcatat
    46441 tgggcgcggc tgtaacgcgg atgcagcgcc gacagcttga ttgaaatgcc cggaccttca
    46501 tagatgccac gaccattgga cgctttacca atggcgtgaa tcgcctgctg ataggaaacc
    46561 atatacgctt gtgcatctgc ggcagtcagt gcagcttcgc caagcatatc gtaagagtaa
    46621 cggaaacctt tctcttccag cttgcgggca ttggctaacg cttccgcgat ggtttcgcca
    46681 gtgacgaact gctcgcccat caggcgcatc gccatatcca cacctttgcg gatcagcggt
    46741 tcaccgcttt taccgataat gcggttcagc gagcgggaga ggctggcttc gttatgggtg
    46801 gaaaccagtt tgccagtaaa cagcagcccc caggtggcgg cattaacaaa caaagacggg
    46861 ctacgaccaa tgtgtgattg ccagttaccg ttgctgattt tgtcgcgaat taacgcatca
    46921 cgggtggctt tgtcgggaat acgcaacaac gcttccgcca gacacatcag cgccacgcct
    46981 tcctgcgatg acaacgaaaa ctcctgcaat aacccctgaa ccatacctgc gcgaccactg
    47041 gcgttttttt gattacgcag tttatcggcc agttgatacg ccagtttgtg cgcctgttca
    47101 gcaactggct gcggcaggcg ggcttgttcc agcagcatag aaaccgcttc ggtttccggg
    47161 cggcgatagg ccgctgtgat cgcggcgcgg gaaaccgaca ggggcaaaat ttgctcggca
    47221 aagtcgagga atggttggtg tggttcctct gccggagtcg gtgcttcatc gctctcattg
    47281 gcagcgccag aaagcagcgc aggtagctcc ggcagagtat cgctgttttc cagttgttcg
    47341 agataagaaa aaatcgcctg cttaattaac cagtgtggcg tgcgatcgat acgtgtcgcg
    47401 gcagacttaa tacgctcacg cgtcgcgtcg tccagcttaa cccccatggt ggtggttccc
    47461 atgccattac tcctgttgtt ctgaaaggtg caacttaacg ttatcgtgaa atatccatga
    47521 tgttgcaact ttgtgcaacc atgttaaatg tgacatgcgt agcaagctta aaaatgaatg
    47581 aaatgttaat aaaagaaatc gatatgacag ggattaaaaa aataactcag actttttctc
    47641 tgcggcagtt aacatttttg aaaggtgcaa ccgcaaaaaa tgtgagagag tgcaacctga
    47701 tgaaaaatag tgtcgctgag cactaaaatt taatgtaaat ggtgtgttaa atcgattgtg
    47761 aataaccagc gcttccggca ggatacggtc gccctggtaa aacataaact ctgttacccc
    47821 gttccggtgg cagatataac ggcaagtttc gacattgccg ataataattt tttggagact
    47881 ttagatggct attagcacac cgatgttggt gacattttgt gtctatatct ttggcatgat
    47941 attgattggg tttatcgcct ggcgatcaac gaaaaacttt gacgactata ttctgggcgg
    48001 tcgtagcctt gggccattcg tgacggcatt atcggcgggt gcgtcggata tgagcggctg
    48061 gctgttaatg gggttgccgg gcgcggtttt tctttccggg atttccgaaa gttggatcgc
    48121 cattggcctg acattaggcg cgtggatcaa ctggaagctg gtggcggggc ggttgcgtgt
    48181 gcataccgaa tacaacaata acgccttaac attgccggat tatttcaccg ggcgctttga
    48241 agataaaagc cgcattttgc gcattatctc cgcgctggtg attttgctgt tcttcaccat
    48301 ttattgcgct tcgggcattg tggcaggcgc gcgtctgttt gaaagtacct ttggcatgag
    48361 ctacgaaacg gctctgtggg cgggagctgc ggcgacgatc ctttacacct ttattggcgg
    48421 tttcctcgcg gtgagctgga ctgacactgt acaggccagc ctgatgattt ttgccctgat
    48481 cctgacgccg gttatcgtca ttatcagtgt cggtggcttt ggtgactcgc tggaagtgat
    48541 caaacaaaag agcatcgaaa acgttgatat gctcaaaggg ctgaactttg tcgccattat
    48601 ctcactgatg ggttgggggc tgggttactt cgggcagccg cacatcctgg cgcgttttat
    48661 ggcggcagac tctcaccaca gcattgtcca tgcgcgtcgt atcagtatga cctggatgat
    48721 cctctgcctg gcaggggcgg tggctgttgg cttctttggg attgcttact ttaatgagca
    48781 cccggcggta gcaggcgcgg taaaccagaa cgccgagcgc gtgtttatcg aactggcgca
    48841 aattctgttt aacccgtgga ttgccgggat tctgctgtcg gcgattctgg cggcggtaat
    48901 gtcaacctta agttgccagc tgctggtatg ttccagtgca attaccgaag atttatacaa
    48961 agcgtttctg cgtaaacagg ccagccagaa agagctggtg tgggtaggac gtgtgatggt
    49021 gctggtggtg gcgctggtgg cgattgcgct ggcggcaaac ccggaaaacc gcgtgctggg
    49081 cttagtgagc tacgcgtggg caggctttgg cgcggcgttt ggtccggtgg tgctgttctc
    49141 ggtgatgtgg tcacgcatga cgcgcaacgg tgcgctggcg gggatgatca tcggtgcgct
    49201 gacggttatc gtctggaagc agttcggctg gctgggactc tacgaaatta ttccgggctt
    49261 catcttcggc agtatcggga ttgtagtgtt tagtttgctg ggtaaagcgc cgtcagcggc
    49321 gatgcaaaaa cgctttgccg aggccgatgc gcactatcat tcggctccgc cgtcacggtt
    49381 gcaggaaagc taagggactt agcctgcggc ggttttgttt ggcttcagca gcgggttgcg
    49441 ctcccttaat gtgcctcgcc atataaattg aatggtgcag ggagcgcgca gggggcggcc
    49501 aatcgccgcg gccccctgca cccccgggct ctggcgaaca aaatcgccgc tgcgtggtac
    49561 cttcagctta tccccttcgg ctaccgggtc gggcgcgagg taacgtcctg taagacgcgc
    49621 cctcagccca catccatgtg ggctgccccg gccttcgggg aacgtttcag cgattttgac
    49681 gccaccaaca cccgcgctgt tcttatgttc cgggcaaaaa gttagatttg ataatcgagg
    49741 atggacgaaa ttgcttgata cacccgctta tcagttttac atggaagttt tgatgtattg
    49801 agtcaggacg gttttgtcgg ctggatacgg cgtttacgcg gcatccggca agaacacatg
    49861 gctcgttgta aacaatccca tctttgtacc tggaataatc gtttatatcc cctggcatta
    49921 cctctctttg tttacatttc aacatcattt tataaacatt ccgcttgtat ttttctttac
    49981 cgtaatgata atcgctatca ctgcgattta cttttctttg catagattga ctcagaaaaa
    50041 cgtttaagga tgggtggcat gtttgttccg tttctcatta tgttgcgcga aggacttgaa
    50101 gccgcgctga ttgtcagttt gattgccagc tatcttaagc gtacccagcg aggccgatgg
    50161 attggtgtga tgtggattgg cgtgttgctt gccgctgcgt tgtgcctggg cttggggatc
    50221 ttcattaacg aaaccaccgg cgaatttccg caaaaagagc aggaactgtt tgaaggtatc
    50281 gtggcggtga tcgccgtggt gatccttacc tggatggttt tctggatgcg caaagtgtcg
    50341 cgcaacgtca aagtacaact ggagcaggca gtcgatagcg cattgcagcg tggaaatcat
    50401 catggctggg cgctggtgat gatggtcttt tttgccgttg caagggaagg gctggagtcg
    50461 gtctttttcc tgctggcggc atttcaacaa gatgtcggga tctggccgcc gctgggtgca
    50521 atgctcggtc ttgctactgc cgtggtgcta ggcttcttgc tctactgggg cggtattcgc
    50581 ctcaatcttg gtgcattttt taaatggacc agcctgttta ttctcttcgt cgccgcaggg
    50641 ctggcagctg gtgccattcg cgcatttcat gaagccggat tgtggaacca ctttcaggaa
    50701 atcgccttcg atatgagtgc ggtgctctca actcactcgc tgtttggcac gctgatggaa
    50761 gggatttttg gctatcagga agcgccgagc gtcagcgaag tcgccgtctg gtttatttat
    50821 ctcatcccgg cgctggtggc atttgctctg ccaccacgcg caggggcgac agcgtctcgc
    50881 tccgcgtaac aaatacgacg caaactcttg cttagttaca acatacttta aagggatagt
    50941 ctcgtcatga ccattaactt ccgccgtaac gcattgcagt tgagcgtggc tgcgctgttt
    51001 tcttctgctt ttatggctaa cgccgctgat gtgacgcagg tcaaagtgac cgtgacggat
    51061 aagcagtgcg aaccgatgac cattacggtt aacgccggga aaacacagtt cattattcag
    51121 aaccatagcc agaaggcgct ggagtgggag atcctcaaag gcgtgatggt ggtggaagag
    51181 cgggaaaata tcgcccctgg ttttagccag aaaatgacgg cgaatttaca gcctggcgaa
    51241 tacgatatga cctgcggtct gctgactaac ccgaaaggga agttgatcgt caaaggtgag
    51301 gcaacggcgg atgcggcgca aagtgatgcg ctgttaagtc ttggtggtgc aattactgca
    51361 tataaagcgt atgtcatggc ggaaaccacg cagctggtga ccgacaccaa agcctttacc
    51421 gacgcgatta aagcaggcga tatcgaaaaa gcgaaagcac tgtatgcgcc gacgcgccag
    51481 cactatgagc gcattgaacc gattgctgaa ctgttctccg atctggatgg cagcattgac
    51541 gcccgtgaag atgattacga gcaaaaagcc gctgatccaa aattcaccgg tttccaccgt
    51601 ctggaaaaag cattgtttgg cgacaacacc accaaaggca tggatcagta cgctgaccag
    51661 ctttataccg atgtggtcga tttgcaaaaa cgcatcagtg aactggcttt cccaccttca
    51721 aaagtggtcg gcggtgcagc cggactgatt gaggaagtgg cagccagcaa aatcagcggt
    51781 gaagaagatc gctacagcca caccgatctg tgggatttcc aggctaacgt tgaaggctcg
    51841 cagaaaattg tcgatctgct gcgtccacaa ctgcaaaaag ccaacccgga actgttggca
    51901 aaagtcgatg ccaactttaa aaaggtcgat accattctgg cgaaataccg tactaaagac
    51961 ggttttgaaa cctacgacaa attgaccgat gccgaccgga atgcactgaa aggaccgatt
    52021 actgcgctgg cggaagatct ggcgcaactt cgcggtgtgc tgggattgga ttaagcatta
    52081 tgcagtatga agataaaaac ggcgtgaatg aaccgtcacg ccgacgttta ctgaaaggca
    52141 ttggtgcgct ggcgctggcg ggaagttgtc cggtcgctca tgcacaaaaa acgcaaagtg
    52201 cgccgggtac gctttcaccg gatgcacgca atgagaaaca gccgttttat ggtgagcatc
    52261 aggcagggat cctgacgcca caacaggccg caatgatgct ggtggcgttt gatgtgcttg
    52321 ccagcgataa agccgatctt gaacggttgt ttcgcttgtt gactcagcgt tttgcttttc
    52381 tgactcaggg cggagcggca ccagaaacgc caaatccgcg cctgccacca ctcgattccg
    52441 gcattcttgg cggctacatt gcgcccgata atctcaccat cacgttatcg gtgggtcact
    52501 cattgtttga tgagcgcttt ggccttgcgc cacagatgcc gaaaaagttg cagaagatga
    52561 cgcgttttcc caacgactcg ctggatgcgg cgttatgtca tggtgatgtg ttgctacaaa
    52621 tttgcgccaa cacccaggac acggtgatcc atgcactgcg cgatatcatc aaacatacgc
    52681 cggatttgct cagcgtgcgc tggaagcggg aagggtttat ttccgatcac gcggcgcgta
    52741 gtaaaggcaa agagacgccg attaatttgc tgggtttcaa agacggcact gccaatcccg
    52801 atagccagaa tgataagttg atgcaaaaag tggtgtgggt aacggcagat cagcaggagc
    52861 cagcgtggac aatcggtggc agctatcagg cagtacgctt gattcagttt cgagtggaat
    52921 tttgggacag aactccgctg aaagaacagc agacgatttt tggtcgtgat aagcaaaccg
    52981 gtgcgccgct gggaatgcag catgagcatg atgttcccga ttacgccagc gatccggaag
    53041 ggaaggtgat tgcgctggac agccatatcc ggctggcgaa tccccgcacg gcggagagtg
    53101 agtccagcct gatgctgcgt cgtggctaca gttattcact gggcgtcacc aactccgggc
    53161 aactggatat ggggctactg tttgtctgtt accaacacga tctggaaaaa ggcttcctga
    53221 cagtacaaaa aaggctcaat ggcgaagcgc tggaggaata cgttaaacct atcggcggcg
    53281 gttatttttt tgcgctgccg ggagtgaagg acgcgaacga ttatctcgga agcgcgttat
    53341 tgcgggttta atgtttatag acggataagg cgttcttgcg cagatgcctg atgcgatgct
    53401 tgcgcgtctt cttatgccta caatcggtgc gggttcggta ggcggataag gcgttcacgc
    53461 cgcatccggc gttcgtgcgc agatgcctga tgcgaatcct gctgaaagca cacagctttt
    53521 ttcatatctg tcatcactct gtcatctttc cagtagaaac taatgtcact gaaatggtgt
    53581 tttatggtta aatataagtg aatatattgt tgcaatgaat gcgagatctg ttgtaattat
    53641 taagtagcag cggaagttcc cggcagtgat agtcagtcac tatggagatc gcggatggta
    53701 acgtcctgtt ctggacaatg tcttgttaag ctacgctcca ccacttgcgg agatttctcc
    53761 tccggtagca acttcgtcac tctgtatttt tctcacttcc tctctttact cgtgttaccg
    53821 acgccgcaaa cggtgtgcgt agccgttttt cggcatttta tccaaaagca aactatggct
    53881 tacaaggaag ccaaccctct gatgttcgtg cgcataatcg cgctgccaac ggcgcgtgtg
    53941 atgaatacaa acaactcaag gtgctctcca tgggaagaca aaaagcagtg atcaaagctc
    54001 gtcgcgaggc aaaacgtgtg ctgagacggg attcgcgcag ccataagcag cgtgaagaag
    54061 aatcggtcac ctcgcttgtg cagatgggcg gcgtagaagc cattggtatg gcccgcgaca
    54121 gtcgcgatac ttcgcccatc ctcgcgcgaa atgaagcgca attgcactat ctgaaggcta
    54181 ttgagagtaa gcagctgata ttcgccacgg gcgaagccgg gtgcggaaaa acctggatca
    54241 gcgcagcaaa agcggcagaa gccctgatac ataaggatgt cgacaggatt atcgtcaccc
    54301 gtccagttct gcaagctgat gaagatcttg gcttcttacc tggagatatc gcagaaaagt
    54361 ttgctcccta ttttcgcccg gtctacgacg tgctggtccg gcgcttaggg gcttccttta
    54421 tgcagtactg cctgcgaccg gaaataggga aggtggaaat tgcgccgttc gcctatatgc
    54481 gtggacgtac ctttgaaaat gcagtcgtca ttcttgacga ggcgcagaat gtgactgccg
    54541 cgcaaatgaa aatgttttta acccgcctcg gggagaacgt gacggttatc gtcaacggtg
    54601 atatcacgca atgcgatttg cctcgcggcg tgtgctccgg attaagtgac gcgctggaac
    54661 gtttcgaaga agatgaaatg gttgggatcg tccggttcgg taaagaggac tgcgtacgtt
    54721 cggcactttg ccaacgtacg ctgcatgcct acagttaagt gtattatcgg tgcagagccc
    54781 gggcgaaccg ggctttgttt tatttcaaca gattttcact tcaggttagc gttcaggggg
    54841 gcgactttga gcgtgttgat aagctgaact ggagtctggt ttagatactc ttaccaggca
    54901 tttttcgtca tagcatcaga ccagtgtaat actgccagcg cggagagttt ttcttccaga
    54961 agccaaaaaa atgagcatct gcctatcttt tttcggccat tcaacctgta caggagatct
    55021 ggtgccaatt ttgcttatga ggtttaatca acaatgggct atactttgac tgactgttta
    55081 acaccgaata tattcctctg atttattctt tagggataat attataaatg taaggttaat
    55141 cttaattaat tctttgctaa tattgtgtgt tgttattttt ttgtggaagg caagccgcaa
    55201 tggctaaata gggatggttg taataatcaa aacatgatac taactatata ttgatcgttg
    55261 cattgttggc atgtaacacg ctgcagcgag taacattggt aatgaaatat aatcaacatg
    55321 gataggtgaa aattaattat gagctttatg gcttttgtaa cttgtttgac aattgcattt
    55381 atcgccggtt ttgctgttgc cagaaaattg acactccgaa aagttgcctc atcggtaatc
    55441 atcgctaaat cgcaacccgt cccttcatct gcaacttccc ctgccgctaa ggcgtcgaca
    55501 aggagctata taccgcagaa aacctatcgt gactctcgtt ggcaagagcg agatgagtta
    55561 tttcagggaa tggttgccga aggtatttta ttggaaagaa aagagtggtt aatcacgcgt
    55621 tgtgaaaaaa tgtatttcga taatctggaa aaatggtttg gacattattg tcatatccat
    55681 tgccaggtct ctctggggcg cttgatcaac tttccagaac aacctgagtt tagcgaagaa
    55741 gaaagaaagc gtttctttgc aatttataat ggcatggcaa tggattatgt tctggtttca
    55801 aagaagaaca ataaaatagt ctgtgttatt gagctggatg acccgagtca tgaaagggcg
    55861 gatcgcatta ttcgtgaccg ttggttggat acaattatga ctcttgcacg ggtgcctttt
    55921 gtacgagctt ctgtcactaa attggaggaa attcctccgg tctgggagac tcgtgacaaa
    55981 attcgcccat tagtcgcggc gacaaagagt gaaccggtgc attaactaag catcccggtc
    56041 ggggcttctc atccccccgg tgtgtgcaat atacgaaaaa aagcccgtac cttcgtacga
    56101 gctcttcttt aaagatggcg gtgagggggg gattgactcg cttcgctcgc cctgcgggca
    56161 gcccgctcac tgcgttcacg gtctgt
//
LOCUS       DAZGOB010000017        56075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGOB010000017 DAZGOB010000000
VERSION     DAZGOB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.017"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            209..1303
                     /gene="gcvT"
                     /locus_tag="ACHLIF_001067"
     CDS             209..1303
                     /gene="gcvT"
                     /locus_tag="ACHLIF_001067"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO9605122.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1327..1716
                     /gene="gcvH"
                     /locus_tag="ACHLIF_001068"
     CDS             1327..1716
                     /gene="gcvH"
                     /locus_tag="ACHLIF_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO9605123.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1835..4708
                     /gene="gcvP"
                     /locus_tag="ACHLIF_001069"
     CDS             1835..4708
                     /gene="gcvP"
                     /locus_tag="ACHLIF_001069"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO9605124.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            4969..5712
                     /gene="ygfF"
                     /locus_tag="ACHLIF_001070"
     CDS             4969..5712
                     /gene="ygfF"
                     /locus_tag="ACHLIF_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9605125.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYTASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5769..7202)
                     /gene="bglA"
                     /locus_tag="ACHLIF_001071"
     CDS             complement(5769..7202)
                     /gene="bglA"
                     /locus_tag="ACHLIF_001071"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO9605126.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRIHPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            7247..7558
                     /gene="yqfB"
                     /locus_tag="ACHLIF_001072"
     CDS             7247..7558
                     /gene="yqfB"
                     /locus_tag="ACHLIF_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO9605127.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7722..8381
                     /gene="yqfA"
                     /locus_tag="ACHLIF_001073"
     CDS             7722..8381
                     /gene="yqfA"
                     /locus_tag="ACHLIF_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO9605128.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8577..9557)
                     /gene="ygfZ"
                     /locus_tag="ACHLIF_001074"
     CDS             complement(8577..9557)
                     /gene="ygfZ"
                     /locus_tag="ACHLIF_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO9605129.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9800..10066
                     /gene="sdhE"
                     /locus_tag="ACHLIF_001075"
     CDS             9800..10066
                     /gene="sdhE"
                     /locus_tag="ACHLIF_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO9605130.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10047..10454
                     /gene="ygfX"
                     /locus_tag="ACHLIF_001076"
     CDS             10047..10454
                     /gene="ygfX"
                     /locus_tag="ACHLIF_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO9605131.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10494..11015)
                     /gene="fldB"
                     /locus_tag="ACHLIF_001077"
     CDS             complement(10494..11015)
                     /gene="fldB"
                     /locus_tag="ACHLIF_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO9605132.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11127..12023
                     /gene="xerD"
                     /locus_tag="ACHLIF_001078"
     CDS             11127..12023
                     /gene="xerD"
                     /locus_tag="ACHLIF_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO9605133.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLRAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12048..12758
                     /gene="dsbC"
                     /locus_tag="ACHLIF_001079"
     CDS             12048..12758
                     /gene="dsbC"
                     /locus_tag="ACHLIF_001079"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO9605134.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12764..14497
                     /gene="recJ"
                     /locus_tag="ACHLIF_001080"
     CDS             12764..14497
                     /gene="recJ"
                     /locus_tag="ACHLIF_001080"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO9605135.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14588..15686
                     /gene="prfB"
                     /locus_tag="ACHLIF_001081"
     CDS             join(14588..14662,14664..15686)
                     /gene="prfB"
                     /locus_tag="ACHLIF_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO9605136.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15696..17213
                     /gene="lysS"
                     /locus_tag="ACHLIF_001082"
     CDS             15696..17213
                     /gene="lysS"
                     /locus_tag="ACHLIF_001082"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO9605137.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGVAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17256..17804)
                     /gene="idi"
                     /locus_tag="ACHLIF_001083"
     CDS             complement(17256..17804)
                     /gene="idi"
                     /locus_tag="ACHLIF_001083"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO9605138.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHRAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYGLGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17859..17930
                     /locus_tag="ACHLIF_001084"
     CDS             17859..17930
                     /locus_tag="ACHLIF_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605139.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17927..18052)
                     /gene="yqfG"
                     /locus_tag="ACHLIF_001085"
     CDS             complement(17927..18052)
                     /gene="yqfG"
                     /locus_tag="ACHLIF_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO9605140.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18054..19502)
                     /gene="uacT"
                     /locus_tag="ACHLIF_001086"
     CDS             complement(18054..19502)
                     /gene="uacT"
                     /locus_tag="ACHLIF_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO9605141.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            19938..21857
                     /gene="ygfT"
                     /locus_tag="ACHLIF_001087"
     CDS             19938..21857
                     /gene="ygfT"
                     /locus_tag="ACHLIF_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO9605142.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21857..22345
                     /gene="ygfS"
                     /locus_tag="ACHLIF_001088"
     CDS             21857..22345
                     /gene="ygfS"
                     /locus_tag="ACHLIF_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9605143.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22381..23748)
                     /gene="ghxQ"
                     /locus_tag="ACHLIF_001089"
                     /gene_synonym="ygfQ"
     CDS             complement(22381..23748)
                     /gene="ghxQ"
                     /locus_tag="ACHLIF_001089"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO9605144.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(23784..25100)
                     /gene="guaD"
                     /locus_tag="ACHLIF_001090"
     CDS             complement(23784..25100)
                     /gene="guaD"
                     /locus_tag="ACHLIF_001090"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO9605145.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWARLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(25118..26518)
                     /gene="xanQ"
                     /locus_tag="ACHLIF_001091"
     CDS             complement(25118..26518)
                     /gene="xanQ"
                     /locus_tag="ACHLIF_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO9605146.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(26683..29553)
                     /gene="xdhD"
                     /locus_tag="ACHLIF_001092"
     CDS             complement(26683..29553)
                     /gene="xdhD"
                     /locus_tag="ACHLIF_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO9605147.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGVPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(29550..30329)
                     /gene="ygfM"
                     /locus_tag="ACHLIF_001093"
     CDS             complement(29550..30329)
                     /gene="ygfM"
                     /locus_tag="ACHLIF_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO9605148.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(30380..31708)
                     /gene="ssnA"
                     /locus_tag="ACHLIF_001094"
     CDS             complement(30380..31708)
                     /gene="ssnA"
                     /locus_tag="ACHLIF_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO9605149.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(31711..34809)
                     /gene="ygfK"
                     /locus_tag="ACHLIF_001095"
     CDS             complement(31711..34809)
                     /gene="ygfK"
                     /locus_tag="ACHLIF_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO9605150.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(35131..35709)
                     /gene="mocA"
                     /locus_tag="ACHLIF_001096"
     CDS             complement(35131..35709)
                     /gene="mocA"
                     /locus_tag="ACHLIF_001096"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO9605151.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            35812..36582
                     /gene="yqeC"
                     /locus_tag="ACHLIF_001097"
     CDS             35812..36582
                     /gene="yqeC"
                     /locus_tag="ACHLIF_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO9605152.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            36630..38255
                     /gene="yqeB"
                     /locus_tag="ACHLIF_001098"
     CDS             36630..38255
                     /gene="yqeB"
                     /locus_tag="ACHLIF_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO9605153.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSTA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(38296..39228)
                     /gene="arcC"
                     /locus_tag="ACHLIF_001099"
     CDS             complement(38296..39228)
                     /gene="arcC"
                     /locus_tag="ACHLIF_001099"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO9605154.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALLDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(39276..40661)
                     /gene="hyuA"
                     /locus_tag="ACHLIF_001100"
     CDS             complement(39276..40661)
                     /gene="hyuA"
                     /locus_tag="ACHLIF_001100"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO9605155.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(40714..41925)
                     /gene="ygeY"
                     /locus_tag="ACHLIF_001101"
     CDS             complement(40714..41925)
                     /gene="ygeY"
                     /locus_tag="ACHLIF_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO9605156.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(41983..43179)
                     /gene="dpaL"
                     /locus_tag="ACHLIF_001102"
     CDS             complement(41983..43179)
                     /gene="dpaL"
                     /locus_tag="ACHLIF_001102"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO9605157.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(43237..44424)
                     /gene="ygeW"
                     /locus_tag="ACHLIF_001103"
     CDS             complement(43237..44424)
                     /gene="ygeW"
                     /locus_tag="ACHLIF_001103"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO9605158.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            44903..46681
                     /gene="ygeV"
                     /locus_tag="ACHLIF_001104"
     CDS             44903..46681
                     /gene="ygeV"
                     /locus_tag="ACHLIF_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO9605159.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(46721..47200)
                     /gene="xdhC"
                     /locus_tag="ACHLIF_001105"
     CDS             complement(46721..47200)
                     /gene="xdhC"
                     /locus_tag="ACHLIF_001105"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO9605160.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(47197..48075)
                     /gene="xdhB"
                     /locus_tag="ACHLIF_001106"
     CDS             complement(47197..48075)
                     /gene="xdhB"
                     /locus_tag="ACHLIF_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO9605161.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(48086..50383)
                     /gene="xdhA"
                     /locus_tag="ACHLIF_001107"
     CDS             complement(48086..50383)
                     /gene="xdhA"
                     /locus_tag="ACHLIF_001107"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO9605162.1"
                     /translation="MEAREATATGESCMRVNAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            50798..51553
                     /gene="actS"
                     /locus_tag="ACHLIF_001108"
     CDS             50798..51553
                     /gene="actS"
                     /locus_tag="ACHLIF_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO9605163.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            51632..51705
                     /locus_tag="ACHLIF_001109"
     tRNA            51632..51705
                     /locus_tag="ACHLIF_001109"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51664..51666,aa:Gly,seq:ccc)
     gene            51840..53447
                     /locus_tag="ACHLIF_001110"
     CDS             51840..53447
                     /locus_tag="ACHLIF_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9605164.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPSASYEELREHLKTIVEWELGVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLKGDNSGLLSYLE
                     PETGSLRPSVSLSVSSEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTAGKPYHYYVTALAAITGARLNEVAQLQVKDVRATEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTAGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGLNG"
     gene            53440..54099
                     /locus_tag="ACHLIF_001111"
     CDS             53440..54099
                     /locus_tag="ACHLIF_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFO9605165.1"
                     /translation="MAEYYPAVFEAEAFNCPHCGVYARQFWRRMYGSARELAQRTTEF
                     RMSTCSHCGDDAYWYDGNMIIPAAGNVELPNPDMPDNCKSDYMEARSIINLSPKGAAA
                     LLRLCLQKLMVHLGEPGENINKDIRSLVQKGLPVRIQQAADICRIVGNQAVHPGEISL
                     DDDPQLAHGLFKLLNIIVDDRITRPKEIEAMFQSMPEGPRQGIENQDRQAREQQQAAN
                     E"
     gene            55008..55736
                     /locus_tag="ACHLIF_001112"
     CDS             55008..55736
                     /locus_tag="ACHLIF_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9605166.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPAIVDKLRGILIA
                     FTGTDQQKQEHLLKQAQSGKLEGL"
     gene            55738..55938
                     /locus_tag="ACHLIF_001113"
     CDS             55738..55938
                     /locus_tag="ACHLIF_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605167.1"
                     /translation="MIDPRTPIGRATLRYRGLPTRHLLSLLRLGVDNPDRPYYSRDEL
                     IAMLVDRDLNNQLRRAFAKLES"
BASE COUNT        13738 a        14022 c        14684 g        13631 t
ORIGIN      
        1 tttttattct gttgcgattt tggcgttttt taaccataag ctaatgtgat gatcaatttt
       61 accttatggt taacagtctg tttcggtggt aagttcaggc aaaagagaac gattgcgttg
      121 gggaccggga gtggctccga tgctgggttt cgtggtgata atttcaccat gaaaaagttg
      181 tcagccccgc ttattcaatg aggacaagat ggcacaacag actcctttgt acgaacaaca
      241 cacgctttgc ggcgctcgca tggtggattt ccacggctgg atgatgccgc tgcattacgg
      301 ttcgcaaatc gacgaacatc atgcggtacg taccgatgcc ggaatgtttg atgtgtcaca
      361 tatgaccatc gtcgatcttc gcggcagccg cacccgggag tttctgcgtt atctgctggc
      421 gaacgatgtg gcgaagctca ccaaaagcgg caaagccctt tactcgggga tgttgaatgc
      481 ctctggcggt gtgatagatg acctcatcgt ctactacttt actgaagatt tcttccgcct
      541 cgttgttaac tccgccaccc gcgaaaaaga cctctcctgg attacccaac acgctgaacc
      601 tttcggcatc gaaattaccg ttcgtgatga cctttccatg atcgccgtac aagggccgaa
      661 tgcgcaggca aaagctgcca cactgtttaa tgatgctcag cgtcaggcgg tggaagggat
      721 gaaaccgttc tttggcgtgc aggcgggcga tctgtttatt gccaccactg gttataccgg
      781 tgaagcgggc tatgaaattg cgctgcccaa tgaaaaagcg gccgatttct ggcgtgcgct
      841 ggtggaagcg ggcgttaagc catgcggtct gggcgcgcgt gacacgctgc gtctggaagc
      901 gggcatgaat ctttatggtc aggagatgga cgaaactatt tctcctttag ccgccaacat
      961 gggctggact atcgcctggg aaccggcaga tcgtgacttc atcggtcgtg aagccctgga
     1021 agtgcagcgt gagcatggca cagaaaaact ggttggtctg gtgatgaccg aaaaaggcgt
     1081 gctgcgtaat gaactgccgg tacgttttac cgatgcgcag ggcaaccagc atgaaggcat
     1141 tatcaccagc gggactttct ccccgacgct gggttacagc attgcgctgg cacgcgtgcc
     1201 ggaaggtatt ggcgaaacgg cgattgtgca aattcgcaac cgtgaaatgc cggttaaagt
     1261 gacgaaacct gtttttgtgc gtaacggtaa agccgtcgcg tgatttactt ttttggagat
     1321 tgattgatga gcaacgtacc agcagaactg aaatacagca aagaacacga atggctgcgt
     1381 aaagaagccg acggcactta caccgttggt ataaccgaac atgcccagga gctgttaggc
     1441 gatatggtgt ttgttgacct gccggaagtg ggcgcaacgg ttagcgcggg cgatgactgc
     1501 gcggttgccg aatcagtaaa agcggcgtca gacatttatg cgccagtaag cggtgaaatc
     1561 gtggcggtaa acgacgcact gagcgattcc ccggaactgg tgaacagcga accgtatgca
     1621 ggtggctgga tctttaaaat caaagccagc gatgaaagcg aactggaatc actgctggat
     1681 gcgaccgcat acgaagcatt gttagaagac gagtaacggc tttattcctc ttctgcggga
     1741 gaggatcagg gtgaggaaaa tttatgcctc accctcactc tcttcgtaag gagagaggtt
     1801 cacaattcac tgcacgtttc aggaaccatc gctcatgaca cagacgttaa gccagcttga
     1861 aaacagcggc gcttttattg aacgccatat cggaccggac gccgcgcaac agcaagaaat
     1921 gctgaatgcc gttggtgcac aatcgttaaa cgcgctgacc ggccagattg tgccgaaaga
     1981 tattcagctt gcgaccccgc cgcaggttgg cgcaccggcg accgaatacg ccgcactggc
     2041 agaactcaag gctattgcca gtcgcaataa acgcttcacg tcttacatcg gcatgggtta
     2101 caccgccgtg cagctaccgc cggttatcct gcgtaacatg ctggaaaatc cgggctggta
     2161 taccgcgtac actccgtatc aacctgaagt ctcccagggc cgccttgaag cactgctcaa
     2221 cttccagcag gtaacgctgg atttgactgg actggatatg gcctctgctt ctcttctgga
     2281 cgaggccacc gctgccgccg aagcaatggc gatggcgaaa cgcgtcagca aactgaaaaa
     2341 tgccaaccgc ttcttcgtgg cttccgatgt gcatccgcaa acgctggatg tggtccgtac
     2401 tcgtgccgaa acctttggtt ttgaagtgat tgtcgatgac gcgcaaaaag tgctcgacca
     2461 tcaggacgtc ttcggcgtgc tgttacagca ggttggcaca accggtgaaa tccacgacta
     2521 caccgcgctt attagcgaac tgaaatcacg caaaattgtg gtcagtgtcg ccgccgatat
     2581 tatggcgctg gtgctgttaa ctgcgccggg taaacagggc gcggatattg tttttggttc
     2641 ggcgcaacgc ttcggcgtgc cgatgggcta cggtggccca cacgcggcct tctttgcggc
     2701 gaaagatgaa tacaaacgct caatgccggg ccgtattatc ggtgtatcga aagatgcagc
     2761 tggcaatacc gcgctgcgca tggcgatgca gactcgcgag caacatattc gccgtgagaa
     2821 agcgaactcc aacatttgta cttcccaggt gctgctggca aacatcgcca gcctgtatgc
     2881 cgtttatcac ggcccgattg gcctgaaacg tatcgctaac cgcattcacc gtctgaccga
     2941 tatcctggcg gcgggcctgc aacaaaaagg tctgaaactg cgccatgcgc actatttcga
     3001 caccttgtgt gtggaagtgg ccgacaaagc gggcgtactg acgcgtgccg aagcggctga
     3061 aatcaacctg cgtagcgata ttctgaacgc ggttgggatc acccttgatg aaacaaccac
     3121 gcgtgaaaac gtaatgcagc ttttcagcgt actactgggc gataaccacg gcctggacat
     3181 cgacacgctg gacaaagacg tggctcacga cagccgctct atccagcctg cgatgctgcg
     3241 cgacgacgaa atcctcaccc atccggtgtt taatcgctac cacagcgaaa ccgaaatgat
     3301 gcgctatatg cactcgctgg agcgtaaaga tctggcgctg aatcaggcga tgatcccgct
     3361 gggttcctgc accatgaaac tgaacgccgc cgccgagatg atcccaatca cctggccgga
     3421 atttgccgaa ctgcacccgt tttgtccgcc ggaacaggct gaaggttatc agcagatgat
     3481 tgcgcagctg gctgactggc tggtgaaact gaccggttac gacgccgttt gtatgcagcc
     3541 gaactccggc gcacagggcg aatacgcggg cctgctggcg attcgtcatt atcatgaaag
     3601 ccgcaacgaa gggcatcgcg atatctgcct gatcccggct tctgcgcacg gaactaaccc
     3661 cgcttctgca catatggcag gaatgcaggt ggtggttgtg gcgtgtgata aaaacggcaa
     3721 catcgatctg actgatctgc gcgcgaaagc ggaacaggcg ggcgataacc tctcctgtat
     3781 catggtgact tatccttcta cccacggcgt gtatgaagaa acgatccgtg aagtgtgtga
     3841 agtcgtgcat cagttcggcg gtcaggttta ccttgatggc gcgaacatga acgcccaggt
     3901 tggcatcacc tcgccgggct ttattggcgc ggacgtttcg cacctcaacc tgcataaaac
     3961 tttctgcatt ccgcacggcg gtggtggtcc gggtatggga ccgatcggtg tgaaagcgca
     4021 tctggcaccg tttgtaccgg gtcatagcgt ggtgcaaatc gaaggcatgt taacccgtca
     4081 gggcgcagtt tctgcggcac cgttcggtag cgcctctatc ctgccaatca gctggatgta
     4141 catccgcatg atgggcgcag aagggctgaa aaaagcaagc caggtggcaa tcctcaacgc
     4201 caactatatt gccagccgcc tgcaggatgc cttcccggtg ctgtataccg gtcgcgacgg
     4261 tcgcgtggcg cacgaatgta ttctcgatat tcgcccgctg aaagaagaaa ccggcatcag
     4321 cgagctggat attgccaagc gcctgatcga ctacggtttc cacgcgccga ccatgtcgtt
     4381 cccggtggcg ggtacgctga tggttgaacc gactgaatct gaaagcaaag tggaactgga
     4441 tcgctttatc gacgcgatgc tggctatccg cgcagaaatc gaccaggtta aagccggtgt
     4501 ctggccgctg gaagataacc cgctggtgaa cgcgccgcac attcagaacg aactggtcgc
     4561 cgagtgggcg catccgtaca gccgtgaggt tgcagtattc ccggcaggtg tggcagacaa
     4621 atactggccg acggtgaaac gtctggatga tgtttacggc gaccgtaacc tgttctgctc
     4681 ctgcgtaccg attagcgaat accagtaatt cactgattcg actattttct aaaggcgctt
     4741 cggcgccttt ttagtcagat gacaaagtac aaaagtgctc agacagtccc ctcgcccctt
     4801 tggggagagg gtgaggggaa caggccggca ctggcgcgaa tatttaccct caccccggcc
     4861 ctctccctga aagggcgagg gggaaaagcg tgccaacatt gaagattgag ccagtttgtt
     4921 agcaatctca aagatacgtc aacgaatgaa tttttctcgg aaaaacaaat ggctatagca
     4981 cttgtgaccg gtggcagtcg cggcatcggg cgggcaactg cattactgtt ggcgcaagaa
     5041 gggtatacgg tggcggttaa ttatcagcaa aacctccacg cggcgcagga agtgatgaac
     5101 ttaataacgc aagccggtgg caaagcattc gtgctccagg cggatatcag cgacgaaaac
     5161 caggtcgttg cgatgtttac agcaatcgat cagcacgatg aaccgctagc agcgctggtc
     5221 aataacgccg ggatcttgtt tacccagtgc accgttgaaa accttaccgc agagcgaatc
     5281 aaccgagtac tttccaccaa cgtgacggga tattttctct gctgccgcga ggcggtaaaa
     5341 cgcatggcgc ttaaaaatgg tggcagtggc ggcgctatcg tcaatgtctc ttcggtggcc
     5401 tcacggttgg gttcgccagg ggaatatgtt gattacacgg catcgaaagg ggcgattgat
     5461 acgttaacca ccggactatc gctggaagtc gccgcgcagg ggatccgcgt taactgcgtg
     5521 cggccagggt ttatttatac cgaaatgcac gccagcggcg gcgagcctgg acgcgtcgat
     5581 cgcgttaagt cgaacatccc catgcagcgt ggtggacagg cagaagaggt cgcgcaggcc
     5641 attgtctggc tactaagtga taaagcctct tacgtcacgg gaagttttat cgatttggcg
     5701 ggcgggaaat aaaacaggga agttgtctga ccggatgcaa caagtattgc atccggtact
     5761 tcatcgactt aaagcttctc gccgttgctg gcaatcacct cttggtacca gttaaagctc
     5821 ttcttacgtg aacgcgacat atcgccagca ccgtcgtcat gtttattcac atagataaag
     5881 ccgtagcgtt tgctgtactg cccggtggtg aacgatacgc agtcgatgca gccccacggt
     5941 gtgtagccca tcagatccac gccatcgtaa gtcaccgctt ttttcatctc ttcgatatgg
     6001 gcgcgcaggt agtcaatgcg gtagtcgtcg ttgatgctgc catcttcttc cactttgtcg
     6061 taagcgccaa aaccgttttc gacaataaac agcggcctct gataacgctc atacagttcg
     6121 caaagggcat agcgcagacc tactggatca atctgccagc cccagtcaga cgctttaaca
     6181 tacgggttcg gtacgctgcc ttcgaaacca gagatcgcat cgccggtgcc gccttcggcc
     6241 ttcactgcgt tggtcatgta atagctgaaa ccaagataat cgcaggtgcc ttcacgcagt
     6301 acatcgagat cgccgtcttc cattttgatg ttaaatccgc gacgctccca ctcgttcaac
     6361 acataggacg ggtaatagcc gcgtagctgc acatcggtaa agacgtagcg ttcgcgcatc
     6421 gactcctgag cgaacatcac atcgtccggg ttacagcagt agggatagag cggcaccatc
     6481 gccagcatac agccgacttt catctccggg tgaatgcgac gcgcagcttt caccgccagg
     6541 gcgctggcga caaactggtg atgcagcacc tgatacatcg tctcttccgg gttctcatgc
     6601 tcggtataaa ccacgccgga gcagcagtaa ccgaacagcg gtgcacgcca gttacgctgg
     6661 ttgttaattt cgttgaaggt catccagtat ttgactttat gcttatagcg ttcaaacacc
     6721 acttccgcga aacggacaaa gaaatcaacc actttacggt tggtccagct accgtattgc
     6781 tgcaccagat gcagcggcat ttcaaagtgg gagagggtga tcaccggttc gatgttgtat
     6841 ttcagcagtt catcgaacat atcatcgtag aacttcagcc cttcttcgtt tggctgagct
     6901 tcatcacctt ttggaaaaat acgcgtccag gcgatggaag tccgaaaaca tttgaagccc
     6961 atttcggcaa atagcttgat gtcttccttg tagtgaccat aaaaatcaac ggcttcatgg
     7021 tttggatagt atttgcctgg caagacttct ttggtgattt cgcgcggcac gccgtgtgcg
     7081 ccaccggtca gaacgtcaca aatgctcggc ccttttccgc ctttgttcca gccgccttcg
     7141 acctgatgag cggcaactgc gccgccccat aagaaatctt tcggtaaggt gagttttttc
     7201 actatcatag gctccagatg agtcaattct tacaggaaat agtaccatgc agccaaacga
     7261 catcactttt tttcaacgtt tccaggatga cattctggct gggcgtaaaa ccatcaccat
     7321 ccgcgacgag tctgaatcgc acttcaaaac gggtgatgtg cttcgtgtcg gacgttttga
     7381 agatgacggt tatttttgca cgattgaagt caccgcaacc tcaaccgtaa cgctggatac
     7441 gctgactgaa aaacatgcag agcaggaaaa tatgaccctg actgaactga taaaggtcat
     7501 tgccgacatc tatcccggtc agacacagtt ttatgtgatt gaatttaaat gtctttaaat
     7561 tgaactcgcg cctgttagtt gaaatttcgc atttaatttc atgattttta ggagattagt
     7621 ctcaaatcag aatttatttt agctaacagg tgttcactgg aactattctc agttacgctg
     7681 gagaggtata catgtgcgtg tacgtttctc cggagtaagt tatggttcag aagcccctca
     7741 ttaagcaggg atattcgctg gcagaggaaa ttgccaacag cgtcagtcac ggcattgggt
     7801 tggtgtttgg tatcgttggg ctggtgttgc tactggttca ggcggtggat ctcaatgcca
     7861 gcgccactgc gataaccagt tacagcctct atggcggcag tatgatcctg ctgttcctcg
     7921 cttcgacgct ctatcacgcc attcctcatc aacgggcaaa aatgtggctg aagaaatttg
     7981 accattgcgc tatttacctg ttgattgccg gaacctacac gccgtttttg ctggtggggc
     8041 tggattctcc gttagcgcgc gggttgatga ttgttatctg gagcctggca ttgctgggta
     8101 ttctgtttaa actgaccatc gcgcaccgat tcaaaatttt atctctggtg acctatctgg
     8161 cgatgggctg gctgtcgctg gtggtaattt atgaaatggc agttaagctc gcggcgggca
     8221 gcgttacctt actggcggta ggcggcgtgg tttattcgct cggggtgatt ttctacgtct
     8281 gcaaacgcat tccatacaac catgccatct ggcacggctt cgtgctcggc ggtagtgtgt
     8341 gccactttct ggcgatctat ttgtatattg ggcaggcgta atgggtatgc ctttgtaggc
     8401 ctgataagac gcgccagcgt cgcatcaggc atcggagcac aactgccgga ttcggcgtga
     8461 acgccttatc cgatctacgg gcctcgtccc tgttgtgata aaggcatcaa atcccagaca
     8521 tgataagacg cgtcagcgtc gcatcaggcg ataaatgcct gatgcgataa agagatttac
     8581 tcttcgagcg aatacggcag cggctcgata tgcaatgtat tcgcatcgtc gcgtacgcgg
     8641 aagatgctat ccggttccat atcgttattc atgacgacct gtaccacgac ctgaccatct
     8701 tccagtttta ccgcagccag caccgtaccg gtacgacgcc agttctcgcc cattttcagc
     8761 tctaagtctt caccagcttc cggcagtcgg ctggcgctac ctgccagcaa ccagagcgca
     8821 cgtttattgg caccacggaa ttttgctcgc gccaccatct cttgtccggt ataacagcct
     8881 ttcttaaagc tgataccgcc cagcgcctgg agattggtcg cctgtgggat aaactgcccg
     8941 ctgttggcgg catcaatcac cgggaaaccc gcttcaatgt ttaatgccag ccactgttgg
     9001 ctattgttca gttccgcttc accgcgcagt ttatcggtca gcatattagc agtagcttca
     9061 tcggttacga tcaggaaacg ttctgccggg tgttcaaacc atagcaaagt ggtcgcgcct
     9121 tctttgacta cctgtttttc tttcgaaggc agttcgctaa agagatttgc cagcgcggcg
     9181 cgcgcctgaa aaccggcaac accaagcagc acacgctcgt cgtctggcgc gatggtcact
     9241 ttagagaata ccgcatattt tttcagttca gtcagctgcg gttcacgcac gctgcgccgt
     9301 tcaatccatg caaagccatc gccgtcgcgg aacagacgta aattgctcca cattttacct
     9361 ttggcgtcgc aatgggcggc gagcaggtgc tgattttctg tcatctggct gacatctgct
     9421 gtcacctgac cctgcatata tttttcgctg tccgcgccag taatggtggc aagcgcccag
     9481 tcatcaagcg tcatcagcgt cagcggtaaa cgggcagaag ccgtaggctg acggggagga
     9541 aaaggtgtaa aagccataat agagtcctga gttgcttaac ggcgtattaa tgactaatgg
     9601 taaaagagct atgaggcaat gcaagtgctt tcactacgcg atctgtgcct tctgcgaggc
     9661 gacttccaaa aaaatgaagc cattgaattg ttgtgaatta atttcgcgat cgcgatggtg
     9721 tttactgata ttcccgtaaa agaaggcgaa aaacacgtta caatagcggg gtactggatt
     9781 ttcgtgagac aggaagaaga tggacattaa caacaaagcc cgcattcatt gggcatgccg
     9841 ccgtggtatg cgcgaactcg atatttcaat catgccgttt ttcgaacatg agtacgacag
     9901 cttaagcgat gacgaaaaac gcatctttat tcgtctgctg gaatgtgacg atccggacct
     9961 gtttaactgg ctgatgaatc acggtaaacc ggccgatgca gaactggaaa tgatggtccg
    10021 actcatccag acacggaacc gggaacgtgg tcctgtggca atctgatttg cgcgtctcct
    10081 ggcgcgcaca gtggctttcc ttgctgattc atgggctggt tgccgctgtt attttactca
    10141 tgccctggcc actcagttac accccgttat ggatggtgtt actttcgctg gtggtgtttg
    10201 attgcgttcg cagccagcgg cgtattaatg ctcgccaggg ggaaattcgc ttgttgatgg
    10261 acgggcgttt gcgttggcaa gggcaggagt ggagcatcgt caaagcaccg tggatgatta
    10321 agagcggcat gatgctgcgt ttacgttctg atggcggtaa acggcaacat ttatggctgg
    10381 cagccgacag catggacgaa gctgaatggc gggatttacg gcggattttg ttgcaacaag
    10441 agacgcaaag ataaagatga cggcaaagca tatgcgctaa gccgtcagtg agatcaggcg
    10501 taatgctctg ccatttcgtt gaggatttgc tcgcaccagc tctgaatacg ctcgtcgcta
    10561 aggtcatact ggttagtttc atccagcgcc agacccacga acagttgccc gtcagcaatc
    10621 accggtttcg ggctggtaaa ttcatatcct tccgttggcc agtagccgac gaacttcacg
    10681 cctttggtcg agagtttgtc atgcagcata ccgagcgcat cgaggaacca ctcgccgtat
    10741 cccagttgat cgccaagccc atacagcgca acaattttac cttcaaggtt caggtcgtcg
    10801 agctgatccc agacggcttc ccagtcttcc tggatttcac caaaatccca ggtcgggata
    10861 cccagaatga gcacatcgta ctgctccatt aatttcgggg agtcgtcctt gaggttatgt
    10921 aaggtcacca gttctgggcc gataatatcg cggatttttt ctgccgccat ttcggtgtaa
    10981 caggtgctgg aaccgtaaaa aagacccata ttcataacgt aagcttctcg attctgctgt
    11041 ggcttttgca gctagtgtat cagaatcgct ttagtcacgg cacaataagg cataatggat
    11101 caaatgagtg accataaggg gcgcaagtga aacaggatct ggcacgcatc gagcagtttc
    11161 ttgatgctct gtggctggaa aaaaatctgg ctgaaaatac gttgaacgct taccgtcgcg
    11221 atctgtcaat gatggtggag tggttgcatc accgcgggtt gacgctggcg acggcgcaaa
    11281 gtgacgattt gcaggcatta ctggcagaac ggctggaggg cgggtataaa gccaccagct
    11341 cagcgcgttt gctgagtgca gtgcgccgat tgttccagta tctttatcgc gaaaagtttc
    11401 gtgaagacga tcccagtgcg catctcgctt caccgaaatt gccccagcgt ttgccaaaag
    11461 atttaagcga agcgcaggtc gaacgtttat tacgggcacc attaattgat cagccactgg
    11521 agctacgcga taaagccatg cttgaagtgt tgtatgctac cgggctgcgt gtctctgaac
    11581 tggtcggact gacaatgagt gatatcagcc tgcgtcaggg cgtagtgcgg gtcattggta
    11641 aaggcaacaa agagcgcctg gtaccgttag gtgaagaggc ggtttactgg ctggaaacct
    11701 atctggaaca tgggcgtccg tggctgctga atggtgtgtc aattgacgtg ttgtttccca
    11761 gccagcgtgc gcagcagatg acgcgacaga ccttctggca ccgcattaaa cattatgctg
    11821 tgctggcggg tatcgacagc gaaaagctgt caccgcatgt gttgcgtcac gcttttgcca
    11881 ctcatttatt aaatcatggt gcggatttac gcgtggtgca gatgctactg ggccacagcg
    11941 atctttccac cacgcaaatt tatacgcatg tcgctaccga gcgtctgcgg caacttcatc
    12001 aacagcatca cccgcgggcg tgatgtctga aaagaacggg aagatttatg aagaaaggtt
    12061 ttatgttgtt tactttgtta gcggcgtttt caggttttgc tcaggctgat gacgcggcaa
    12121 ttcaacaaac gttagccaaa atgggcatca aaagcagcga tattcagccc gcgcccgtag
    12181 ctggcatgaa gacagttctg actaacagcg gcgtgttgta catcaccgat gatggtaaac
    12241 atatcattca ggggccaatg tatgacgtta gcggcacggc tccggtcaat gtcaccaata
    12301 agatgctgtt aaagcagttg aatgcgcttg aaaaagagat gatcgtttat aaagcgccgc
    12361 aggaaaaaca cgtcatcacc gtgtttactg atattacctg tggttactgc cacaaactgc
    12421 atgagcaaat ggcagactat aacgcgctgg ggatcaccgt gcgttatctt gctttcccgc
    12481 gccaggggct ggacagcgat gcagagaaag aaatgaaagc tatctggtgt gcgaaagata
    12541 aaaacaaagc gtttgatgat gtgatggcag gtaaaagcgt cgcaccagcc agctgcgacg
    12601 tggatattgc cgaccattac gcacttggcg tccagcttgg cgttagcggt actccggcag
    12661 ttgtgctgag caatggcaca cttgttccgg gttaccagcc gccgaaagag atgaaagaat
    12721 tcctcgacga acaccaaaaa atgaccagcg gtaaataatt cgcgtgaaac aacagataca
    12781 acttcgtcgc cgtgaagtcg atgaaacggc agacttgccc gctgaattgc ctcccttgct
    12841 gcgccgttta tacgccagcc ggggcgtgcg cagtgcgcaa gaactggaac gcagtgttaa
    12901 aggtatgttg ccctggcagc aactgagcgg cgtcgaaaag gccgttgaga tcctttacaa
    12961 cgcttttcgc gaaggaacgc ggattattgt ggtcggtgat ttcgacgccg acggcgcgac
    13021 cagcacggct ctaagcgtgc tggcgatgcg ctcgcttggt tgcagcaata tcgactacct
    13081 ggtaccaaac cgtttcgaag acggttacgg cttaagcccg gaagtggtcg atcaggccca
    13141 tgcccgtggc gcgcagttaa ttgtcacggt ggataacggt atttcctccc atgcgggggt
    13201 tgtacacgct cgctcgttgg gcattccggt tattgttacc gatcaccatt tgccaggcga
    13261 aacattaccc gcggcggaag cgatcattaa ccctaacttg cgcgactgta atttcccgtc
    13321 gaaatcactg gcaggcgtgg gtgtggcgtt ttatctgatg ctggcgctgc gcaccttttt
    13381 gcgcgatcag ggctggtttg atgagcgtgg catcgcaatt cctaacctgg cagaactgct
    13441 ggatctggtc gcgctgggaa cagtggcgga cgtcgtgccg ctggacgcta ataatcgcat
    13501 tctgacctgg caggggatga gtcgcatccg tgccggaaag tgccgtccgg ggattaaagc
    13561 gctgctggaa gtggcaaatc gcgatgcgca aaaactcgcc gccagcgatt taggttttgc
    13621 gctggggcca cgtctcaatg ctgccgggcg actggacgat atgtccgtcg gtgtggcgct
    13681 gttgctgtgc gacaacatcg gcgaagcgcg cgtgctggca aatgaactcg atgcgctaaa
    13741 ccagacgcga aaagagatcg aacaaggaat gcaagttgaa gccctgaccc tgtgcgagaa
    13801 actggagcga agtcgcgaca cgctacccgg cgggctggca atgtatcacc ccgaatggca
    13861 tcagggcgtt gtcggtattc tggcttcgcg catcaaagag cgttttcacc gtccggttat
    13921 cgcctttgcg ccagcaggtg atggtacgct gaaaggttca ggtcgctcca ttcaggggct
    13981 gcatatgcgt gatgcactgg agcgattaga cacactctac cctggcatga tactgaagtt
    14041 tggcggtcat gcgatggcgg cgggtttgtc gctggaagag gataaattcg aactctttca
    14101 acaacggttt ggcgaactgg ttacagagtg gctggaccct tcgctattgc aaggcgaagt
    14161 ggtgtcagac ggtccgttaa gcccggccga aatgaccatg gaagtggcgc agttgctgcg
    14221 cgatgctggc ccgtgggggc agatgttccc ggagccgctg tttgacggtc atttccgtct
    14281 gctgcaacag cggctggtgg gcgaacgtca tttgaaggtg atggtcgaac cggtcggcgg
    14341 cggtccactg ctggatggta ttgcttttaa tgtcgatacc gccctctggc cggataacgg
    14401 cgtgcgtgaa gtgcaactgg cttataagct cgatatcaac gagtttcgcg gcaaccgcag
    14461 cctgcaaatt atcatcgaca atatctggcc aatttagcgt catcttctct ataaaaaaga
    14521 gcgtggattg ggtacaatcc cgctcttatc accgcatttt gactagctca ataaaagaaa
    14581 tcagaccatg tttgaaatta atccggtaaa taatcgcatt caggacctca cggaacgctc
    14641 cgacgttctt agggggtatc tttgactacg acgccaagaa agagcgtctg gaagaagtaa
    14701 acgccgagct ggaacagccg gatgtctgga acgaacccga acgcgcacag gcgctgggta
    14761 aagagcgttc ctccctcgaa gccgttgtcg acaccctcga ccaaatgaaa caggggctgg
    14821 aagatgtttc tggtctgctg gaactggctg tagaagctga cgacgaagaa acctttaacg
    14881 aagccgttgc tgaactcgac gccctggaag aaaaactggc gcagcttgag ttccgccgta
    14941 tgttctctgg cgaatatgac agcgccgact gctacctcga tattcaggcg gggtctggcg
    15001 gtacggaagc acaggactgg gcgagcatgc ttgagcgtat gtatctgcgc tgggctgaat
    15061 cgcgtggttt caaaactgaa atcatcgaag agtcggaagg tgaagtggcg ggtattaaat
    15121 ccgtgacgat caaaatctcc ggcgattacg cttacggctg gctgcgtaca gaaactggcg
    15181 ttcaccgcct ggtgcgtaaa agcccgtttg actccggcgg tcgtcgccac acatcgttca
    15241 gctctgcgtt tgtttacccg gaagttgatg atgatattga tatcgaaatc aacccggcgg
    15301 atctgcgcat tgacgtttat cgcgcgtccg gcgcgggcgg tcagcacgtt aaccgtaccg
    15361 aatctgcggt gcgtattacc cacatcccaa ccgggatcgt gacccaatgc cagaacgacc
    15421 gttcccagca caagaacaaa gaccaggcca tgaagcagat gaaagcgaag ctttatgaac
    15481 tggagatgca gaagaaaaat gccgagaaac aggcgatgga agataacaaa tccgacatcg
    15541 gctggggcag ccagattcgt tcttatgtcc ttgatgactc ccgcattaaa gatctgcgta
    15601 ccggggtaga aacccgcaac acgcaggccg tgctggacgg cagcctggat caatttatcg
    15661 aagcaagttt gaaagcaggg ttatgaggaa ccaacatgtc tgaacaacac gcacagggcg
    15721 ctgacgcggt agtcgatctt aacaatgaac tgaaaacgcg tcgtgagaag ctggcgaacc
    15781 tgcgcgagca gggggttgcc ttcccgaacg atttccgtcg cgatcatacc tctgaccaat
    15841 tgcacgcaga attcgacggt aaagagaacg aagaactgga agcgctgaac atcgaagtcg
    15901 ccgttgctgg ccgcatgatg acccgccgta ttatgggtaa agcgtctttc gttaccctgc
    15961 aggatgtggg tggccgcatt cagctgtacg ttgcccgtga cgatctgccg gaaggcgttt
    16021 acaacgagca gttcaaaaaa tgggacctcg gcgacatcct cggcgcgaaa ggtaaactgt
    16081 tcaaaaccaa aaccggcgaa ctgtctatcc actgcaccga gctgcgtctg ctgaccaaag
    16141 cactgcgtcc gctgccggat aaattccacg gcttgcagga tcaggaagcg cgctatcgtc
    16201 agcgttattt ggatctcatc tctaacgatg aatcccgtaa cacctttaaa gtacgctcgc
    16261 agatcctctc tggtattcgc cagttcatgg tgaaccgcgg ctttatggaa gttgaaacgc
    16321 cgatgatgca ggtgatccca ggcggtgccg ctgcgcgtcc gtttatcacc caccataacg
    16381 cgctggatct cgacatgtac ctgcgtatcg cgccggaact gtacctcaag cgtctggtgg
    16441 tcggtggctt cgagcgtgta ttcgaaatca accgtaactt ccgtaacgaa ggtatttccg
    16501 tacgtcataa cccagagttc accatgatgg aactctacat ggcttacgca gattacaaag
    16561 atctgatcga gctgaccgaa tcgctgttcc gtactctggc acaggatatt ctcggtaaga
    16621 cggaagtgac ctacggcgac gtgacgctgg acttcggtaa accgttcgaa aaactgacca
    16681 tgcgtgaagc gatcaagaaa tatcgcccgg aaaccgacat ggcggatctg gataacttcg
    16741 actctgcgaa agcgattgct gaatctatcg gtatccacgt tgagaagagc tggggtctgg
    16801 gccgtatcgt taccgagatc ttcgaagaag tggcagaagc acatctgatt cagccgacct
    16861 tcattactga atatccggca gaagtttctc cgctggcgcg tcgtaacgac attaacccgg
    16921 aaatcacaga ccgctttgag ttcttcatcg gtggtcgtga aatcggtaac ggctttagcg
    16981 agctgaatga cgcggaagat caggcgcaac gcttcctgga tcaggttgcc gcgaaagacg
    17041 caggtgacga cgaagcgatg ttctacgatg aagattacgt taccgcactg gaacatggct
    17101 taccgccgac agcaggtctg gggattggca tcgaccgtat ggtaatgctg ttcaccaaca
    17161 gccataccat ccgcgacgtt attctgttcc cggcgatgcg tccggtaaaa taagcattac
    17221 gttatgctca caaccccggc aaatgtcggg gttttttaat tgtgctgcgc gaaagcagac
    17281 aacaattttc ttgcttcact attggctgcc tgcatcacca tccacggact gaacgcccac
    17341 ggtgtggcat caataccgtg taatacatct gctaaatcac accattgata atccatcact
    17401 tcatcatcgt tgatctgtaa cgcactggtg gtgcgtgcgg caaataccgg acacacttca
    17461 ttttccacaa tgccattcgg atcggtggcg cgatagcgaa agtcaggata gatagattca
    17521 ggaggcgtaa tttccacgcc aagcccataa cggcaacggc ggatcaccgc gtcttcgttg
    17581 ctttctccca gttgtgggtg cccacaaacc gagttagtcc acacgccagg ccacgctttt
    17641 ttgctcagtg cgcggcgggt aactaataat tgtcctttgg cattaaacag ccaactggag
    17701 aacgcgcgat gtaagcgggt gtctgccgtg tgtgcggcat acttttccag cgtacccgtg
    17761 ggaactccct gtgcattcaa taaaatgacg tgttccgttt gcataatttc tcacatgtaa
    17821 ttctgatcac tcatcattat agataattga agagtgaaat gattaaccaa gtgagcgttt
    17881 atcgacaacc gcccgttttg agcggatgcc gacaggtaaa aaccatttaa aaaagtagca
    17941 tcatatattt gaagcgactt tcaatggcca ttgcaacaca gtcagaaatg acaggaatag
    18001 agagagtaaa aagcaacaac agactgaata tagcgcgcat taaaaaattc atattattct
    18061 ccatgctcat ttttcttcag cttgcgcatc agaagccaca tacgtactgt cctgactttt
    18121 aaatctttat cagagacgga ttccttcacc aggtcagcat gatgctgata gccattaaag
    18181 aagacattca gcacaacggc gctaagggtt gcgagcataa tgccgctatg cagcaacggt
    18241 tgcagtacgg ccggtaactt agaaaagaaa tcgtgagaga gcgtcggagt catgccaacg
    18301 ccgagactga tcgccacaat atagaggttg taacggttgg tggtgtagtt acagcgcgac
    18361 agaattcgaa tccctgtcgc cagtaccatg ccgaacatca ccagcccagc gccgcccagc
    18421 acaaattgcg gaatggaggc taccagcacc gccatttttg gcaccatgcc aaacaggatt
    18481 aatataattc ccgaagaaat acacacccag cggctatgaa cgcgcgtcac gctaaccagg
    18541 ccaacgtttt gagaaaatga cgtgtggggg aagctgttaa acgtgccgcc tatcattgtc
    18601 cctacgccat cgacacgcag cccgcgaata atatcgtgtg aagagagttt acgaccgact
    18661 atttcaccca gtgccaggaa catccccatc gactcgataa acacgatgat taacacggca
    18721 gtcatggtca gaatggaaac gggatcgaaa atcggcatac caaacgacat cggggtgaca
    18781 atcgcaaacc acgaggcatc atgtagcccg gataaattga cttcattcat catccacgaa
    18841 agtaaaaagc caaatacaat ccccagtaat acggcgacgt tggacataaa tcctttcgca
    18901 tagcgagtaa tgagcaagat aaaaattaag acggcaaagg agatacctaa ataaacggga
    18961 ttaccatatt gcggattccc tttacctccg gcggcccagt caatacccac ctgaatgatg
    19021 ctaagcccaa tagaagtaat aaccacaccg gtaaccagtg gcgggaataa aggcatcaag
    19081 cgaccaataa gtggcgctaa taatgtggtg ataaaacccg cggctatagt ggcaccaaat
    19141 atccccagca ggccgatatc cgggttcatc ccaatggcta tcattggtgt tacagcagca
    19201 aaggtcaccg acataatcac cggcaggcgg atccccataa agcggccgat accgatacat
    19261 tgcaataatg tgacgatccc gcagcaaaag agatccgagc taatgagcat cgcaatagct
    19321 tcttttgaga ggcccagtcg gtcaccaatc ataagaggaa cagcgactgc acctgcgtac
    19381 atgacaagga cgtgttgcag accgagtatg atcagctttc ctggtgataa tatgcggtca
    19441 acctcatcag ttgggcggtc ttgcccagaa gatgagggaa gttgggaatc tatggcgctc
    19501 atcagtgttt ctcctttcgc ccgccagagt aaaaagcaac cgtttacgtt aatgaaactg
    19561 tctaaacaca tgctcgtgga gtgccagttt ttgcgaggaa ggggagttag agacaggaat
    19621 tgcggaggca atcacataat cgtggttaaa ccacgaaaat gagaagagaa cctgacaatg
    19681 aaattatctg taattttctc atattgatat ttcattaatc attctggcat gtcgcactct
    19741 cgcatttaat cgtttttatc tggatagcgc tcttttggtc ggcaatgttg aaataatgaa
    19801 agcggcatag attgaggggc aataatttga actcagtcgc aaaattataa ttaaaacggt
    19861 catgctacct aatatatgcc cgtttattgc atcgcgaaat ttgagaagac ataactcatt
    19921 aaatgaaagg gatgcaaatg aataagttta tcgctgctga agctgcggaa tgtataggct
    19981 gtcatgcttg tgaaattgcc tgtgcggtgg cacacaatca agaaaactgg ccgctgagtc
    20041 acagtgactt tcgaccgcgt atccacgttg tagggaaagg ccaggctgcg aatccggtgg
    20101 cctgccatca ctgcaacaat gccccttgcg ttacggcttg tccggttaat gctctgactt
    20161 tccagtccga tagcgtacaa ctggacgagc aaaaatgtat tggctgtaaa agatgcgcaa
    20221 tcgcttgccc ctttggcgtc gttgagatgg tcgatacgat tgcgcagaaa tgcgaccttt
    20281 gtaaccagcg cagttccggc acgcaagcct gtattgaagt ctgcccaaca caggcgttac
    20341 gactgatgga cgataaaggg ttacagtgga taaaggtggc tcgccagcgc aaaacggcag
    20401 caggaaaagc gtcatcagat gctcagccat ctcgcagtgc agcgttgctc cccgttaact
    20461 cgcgtaaagg cgcagataaa atttcagcga gtgaacggaa aacccacttt ggcgaaatat
    20521 attgcgggct ggatccactg caagcgactt atgagagtga ccgctgtgtt tattgtgccg
    20581 aaaaagctaa ctgcaactgg cattgcccgc tgcataacgc tattccggat tacatccgtc
    20641 tggtacagga aggaaagatt attgaagcgg cagaactttg ccaccagacc agttccttac
    20701 cagaaatttg cggcagggta tgtccacagg accgtctttg tgaaggcgca tgtactttga
    20761 aagatcactc gggcgcagtc tctatcggta atctggaacg ctacatcacc gataccgcgc
    20821 tggcgatggg ctggcgtccc gatgtcagca aagttgttcc ccgtagcgaa aaagtggcgg
    20881 tgattggcgc tgggccagca gggttagggt gtgctgatat tctggcgcgc gcgggagttc
    20941 aggttgatgt ctttgatcgc catccagaaa ttggcggtat gctgactttt ggcattcctc
    21001 ctttcaaact cgataaaacg gtattaagcc agcggcgaga gatattcacc gcaatgggaa
    21061 tcgactttca tcttaactgt gaaattggcc gcgatattac ctttagcgat ttaacttctg
    21121 aatatgatgc agttttcatc ggcgtgggga cttacgggat gatgcgagca gatctgccgc
    21181 atgaagatgc gcccggtgtc attcaggctc taccgttcct gactgcccat acccgccagc
    21241 tcatgggatt gccggagtct gaagagtatc cgctgacgga cgtggaaggt aagcgagtcg
    21301 tggtattggg cggtggcgat acgacaatgg attgtttgcg gacttccatc cgcctcaatg
    21361 ccgccagcgt gacctgcgcg tatcgtcgtg atgaagtcag tatgccgggc tcgcgcaaag
    21421 aggtggttaa tgcgcgcgag gaaggtgtcg agtttcagtt caatgttcaa ccgcaatata
    21481 tcgcttgtga cgaagatggg cgtttaactg cggtgggcct gattcgtacc gccatgggtg
    21541 agccggggcc ggatggtcgc cgtcgtcctc gtccggtagc gggttcagag tttgaattgc
    21601 ccgccgatgt tctgattatg gcctttggtt tccaggcgca tgccatgccg tggttgcagg
    21661 gcagcggaat taaactcgat aaatggggcc tgattcaaac cggtgacgtc gggtatttac
    21721 ctacccagac gcatctgaaa aaagtctttg ctggtggtga tgcagttcat ggcgcggatc
    21781 tggttgtcac tgcaatggcc gcaggaaggc aggcggcgcg cgatatgtta actctgtttg
    21841 atacgaaggc atcgtgatga aatcgttaat tatcgttaat ccggctgact gtattggctg
    21901 ccgtacttgt gaagtagcct gtgtggtcgc tcacccttca gaacaggagt tgaatgccga
    21961 tgtcttttta ccccggctga aggtacagcg actggacagc atcagtgcgc cggtgatgtg
    22021 ccatcagtgt gaaaacgccc cttgtgttgg cgcttgcccc gtgggggcgc tgacgatggg
    22081 cgagcaggtg gtgcagacca attctgcccg ttgtattggc tgtcagagtt gcgtcagtgc
    22141 gtgcccgttt ggaatgatca ccattcagtc gttgccgggg gatacccgac aacaaattgt
    22201 gaaatgcgat ctctgtgaac agcgggaaga ggggccagcc tgtgttgaat cttgcccgac
    22261 acaggcgctg cagttgctta ctgaaagaga actcaggcga gtccgccagc agcgtattgt
    22321 tgccagcggt gagaatcctc tgtgaaagaa atctccgcgc tgaaaggcgc ggagaatcta
    22381 ttagattgcc caaccacccg cgtaaaatgc gaccagtgcg gcagtaatga tcaccgtacc
    22441 aatattcaac ttttgccatt cgcgagcaaa gacacggcct acgaccagtg tcacaaagcc
    22501 cagcataata ccggtaacga tattacaagt cagaacgatg aacacggcac acaccaggcc
    22561 agccattgcg tcaataaaat cgttgaaatc cagcttcgag acgttactta acatcagcaa
    22621 acccacgtac atcagagcgg gtgcagtggc gtaaccaggg atcaaaaatg acagcggtga
    22681 taagaacaga atcagcagga ataacgcccc cactacggtt gcggtcaacc ctgttttacc
    22741 cccggcggcg gttcctgccg ctgattcgat ataaaccgct gcgggcgctg cgcccaccag
    22801 accggagaat attgaactta ctgagtcact ggtcagggct ttgccgccat tgatgatctg
    22861 gttgtcttta tccaacaaat tcgcctgacc ggcgacggca cggatggtgc cagtggcgtc
    22921 gaacactgcg gtcatcacca atgccagtac actcggaagt acggttggct ggagtgcgcc
    22981 cataatatcg aggctgaaaa tcagagactt accatcttcg ccagtcagac ttggcatcgc
    23041 caccagaccg tggtatttca ccgctgggtc aaagattaag ccgataatcg aaattgcgat
    23101 aatcaccaac aagatcccgc cgggtacgcg gcacttctcc agaccgaaga tgaccgccag
    23161 ccccagcaag ctcatcatca ccgggaagga ggtaaacgca ccgagcgcca ctggcaagcc
    23221 ttcaatcggg tttttgataa ccatacccac accgttagca gcaatcagca gcagaaacag
    23281 gccgataccg atacctgtac cgtgagcgat acccatcggt aaattacgta agatccaggt
    23341 acgcacaccg gttacggaaa tagcggtgaa gatgacgccc atcagaaaga ccgcgcccag
    23401 tgcgacagga acgctaattt gttgcccgag taccagactg aatgcggtaa atgccgtcaa
    23461 ggaaatcgca caaccaatcg ccattggcaa attggcccac aaccccatca gcaacgagcc
    23521 gaagcccgcg accagacagg tggcaacaaa cacagctgcg ggaggaaaac ctgctttgcc
    23581 cagcattccc ggaacgacga taacggaata aaccatggcc agaaaggtcg ttaagccagc
    23641 cagtacttcc tgacgaacgg tactgccacg agcggtaatt ttaaaataat tatccagcgc
    23701 gccctgtggc tttggtgcgt ccggtgtttg taggatgtct ccagacataa tgatgtcctc
    23761 acgaggatgt taaagtttta ttattagttg cgttcgtaca ccagacgacc atcaacgtag
    23821 gtgcggtaga tcgaacggtc atcgcccaac gtcatcatca cgaacaattt gtcgactaaa
    23881 gaaacagagt tgtcatagcg cagctgctgt agcggagtgg cggtgggttc catcaccacg
    23941 aaatcagcct ctttgccagg taaaaagttg ccaatcaaat cgtcaaggcc cagagatttc
    24001 gctccgccga gcgtggccag gtaaaacgct tcatatgccg agaggcgata gccttgtaat
    24061 tgcaatactt tgtaggcttc gttcagcgtt tgcagcatgt tgaaagtggt tccggcaccg
    24121 atatccgttc ccatgcccac tttaactttc ttctgccatg cttttttcaa gttgaataag
    24181 ccgctgccga ggtaaaggtt ggaggtcgga cagaaagcaa tgctggattt ggtttcgctg
    24241 agacgagccc actctttttc ttcgagatgg acgcagtgag caaagacaca gtttttaccg
    24301 gtcaggccgt actgatggta aacatccaga taaccatcat ggtcaggata aagcgatttc
    24361 acccaggcaa tttcatcttt gttttcacag agatgggtat gtacccacgt atccggatat
    24421 tcttctttca ggcgttgcgc catcgccatc tgttcaggag atgaggtcgg ggcgaagcgt
    24481 ggcgtaatcg catatagcag acgaccattt ttgtgccagc gttcaatcag ttctttgctt
    24541 tggtgatagc tgctttcggc agtgtcgagc agataatccg gtgcgttgcg gtccatcatc
    24601 accttaccgg caatcatacg catattgata tgactggcgg cttcaaacag cgcatcaaca
    24661 gattgcggat gaacagtgcc aaacaccagc gccgtggtgg ttccgttacg taaaagctgc
    24721 ttgatgaaga atgccgacat ttcgcgggcg tactctaaat cctcataacg acgttcagta
    24781 gggaaggtgt gtttattcaa ccactccagc aattgttcac cataggcccc caccatttca
    24841 ctttgcggat aatggatatg tgtatcgaca aagcccggta ctatcagttt gccgcgatag
    24901 tcgcgcacgc gaatagtgtc aggaatttga tgctttccgt cttcccattc gccaaaccat
    24961 tccacttttc cctgtttaat gagtaataaa ccatcctcaa taaaccgcag cgcagaggca
    25021 atctcttccg ggttatcgac cgtacgggtg acatcaataa aactgcctcg taccgctttc
    25081 aacgtgtgtt ctcctgacat cattgactcc tttactgtta atccatctct tccgctgagg
    25141 taataccagg cagaacgttt tcctgtcggt agccaccagg gaggataata tttaataaaa
    25201 tcgcagttaa cccgccagca caaatagggt tttcaactag tacataaata gaggctggca
    25261 atattttaaa aatttcggga tcgtaggaga cgccaagtcc taaacctaaa gaagtggcga
    25321 caataagtgt ttctcggcgc tttaaaccgt tggtgatgat tatgcgaatc ccagcgatgg
    25381 caatcatgga aaacatcaac gtcattgcgc ctcccagaac tgccgaggga atggtcgtga
    25441 agaagccgcc aatcatcgga aacaaaccga ggataaccag cattaccgcg atggttcgcc
    25501 cgacataacg tgaagcgacg ccagtcatct gaataacccc attattttgc gcaaacgtgg
    25561 ttaagggtaa tgacccgaca gcggaggcga taacagaaac cagaccatct gccagcacgc
    25621 cgcctttcag tcgggactga tactcttccc cctgaatggg gcggcgggaa accattgccg
    25681 tggcggtgat atcgccgact gcttccagca cgctaagcag ataaatcgtg ccaaccacca
    25741 ggaactgatg gaagctaaaa ctaaagccgt atttgaacgg atgcgggatg gtgattaacg
    25801 gcaaattgcg catactgctg aaatccacca tgcccaggca taacgatgcg atatagccga
    25861 cacatagccc aatggcgatc cctcccatgc gtagcaacgg actgcgacag cagttaaagc
    25921 cgatcaccac aattaaaacc aataaaccaa cgccgagatg ttcgtaattg ccgaacgtac
    25981 cgctgctttt ggctgcaaat cctccaccaa aatcgataat gccgacttta atcaggctta
    26041 agccgatcat cagtaccaca ataccgctga cggtaggcgt aataacccga cgtaaatagg
    26101 gcaagataaa tgaagatccg acaaccagaa atgcgccaac gaaggagacg ccgagaagcg
    26161 acgacatgat taactcttcg tgaaaaccgt cgcttttcat gctgctgccc agcgcaatca
    26221 tgaccgtaac aaatgaaaaa ttgactgact gaattgagag taggccagaa ccgacgatgc
    26281 cgtagcggtt tacttgtaac caggtaccaa taccagaggc gatcatcgcc atagaaacaa
    26341 gataggcggt tgtttcagcg gaaagctgta aggccgcacc cacgattaac gcgggggtta
    26401 ccatcggaac gaaaattgcc aacagatggg taatggcacc aacgagagcc tgatgaaagg
    26461 gagggcgatc ctccagttca aatataaggt cagaacctgc atggtttata tcagacatcc
    26521 cttcactcct tttaaagtaa atttgatcat ctgaattagt aaaataaatt cccacgaatt
    26581 tggggcgcag gacgccttga gaacggctta tctcattttc gcagtcacta tttagtcatc
    26641 gttcaaacag agaaacaggc cgccgaagcg gccatttatt acttatattt tttccagcgc
    26701 ggtgagtatt ttctccggtg tgaaatgcca ttcgcgtaac cagatgccgc aggcatcgtg
    26761 aattgcggta gcaatcgccg gagctgcgcc atttacacca atttccgaga tcgatttcgc
    26821 cccgaacggg ccgactttat cgtcgctcgg caccaacaca gcgcggaaat cacgcggaat
    26881 gtcaccaatt ttcggtgcgc cgtaactgcg taaatcacgc gttaacgggt gaccttcggc
    26941 gttataaatg atctcttcgc tcatactgtg gccgatagca cgcagggtgg caccgtagat
    27001 ttgtcccaac gctaactctg gattgaccgg tgtaccgcag tccagcaagg cgtagaattt
    27061 atccaggcgg atttcacccg tacgcgtgtt gacggcaact tcagcgaagt ttgcgccata
    27121 cgggaaagcg aaatcaggcg tgatataact gccagttccc accagtgaac caaagccggt
    27181 gccggtttcg cctttatggg caatatcccc gaaactaact tcgcctttct tgccgcgcac
    27241 gacgcccggc gttgctagtt gaacatctgc cactggctca cccaacattt gcgcgccgtg
    27301 gaacagaatt ttctcccgca gattttccgc tgccaaacgc gccgcgttac ccgagaagca
    27361 agtaccggac gaggcatatg cgcctttatc aaacaacgca tgatcggtat caccggagat
    27421 aacatgcacg tcctgcggtg ggcagtgcag cacttctgct gccagtttcg tcactacagt
    27481 atccagacca gtaccaatat ccgcaccgcc agaatgaacg ataaaggtgc cgtccgattc
    27541 cagtttgatc atgcagttag cctgatcgat atccgggata cccgatttct gcatgataat
    27601 ggcgacgccg cgaccgatgt gccagtcact attttgcggt ttcggtgaag accattggat
    27661 catctcgcga ccctgacgca ggatctcttc cagtgcgcag ctggcggcgg aaggaacgga
    27721 ggtcggcgct ttaccttcac cgattgcacc gagaattttc agctcttgcc cttcgtgtac
    27781 ccggttacgt tcgataattt ccagttggtc gatctgtaac tgttcagcca gttccgctaa
    27841 tgccatggtg atagcgaagt tacctttcgg tacgccataa ccctgataag caccatttgg
    27901 gcaaatgttg ctgtagtagg tggtgacctg gaaatcgacg ttatcgcacg gatataacgg
    27961 cagcgacagc gccggtccgt tacacggtac ggtgagtgag tggttgccgt aagggccagt
    28021 gttggcgcgg aaatccatct tcactgccgt caggcgacca tcttttttcg ctcccagttt
    28081 gacggtgact ttcgcgacgt gacgagaggt gttagcaata aactcttctt cacgggtgta
    28141 gcggaacagt accggacgcc cggtcacgca ggttgcccat gcgcacactt cttccagcag
    28201 gatgtcctgt ttggagccaa aaccgccgcc aactcgctct ttaatgacat gaactttatg
    28261 ctgtttcatg cctacgaggc gcgcgacctg gcggcgtaag tgccatggta cctgggtgga
    28321 ggcgtggata accagacgat cgccgtccat ccgggtaaag cagatatgtg tttcagtcgg
    28381 gcactgctgt gcctgcgttg agttataggt tcgctcaatg atcacatcgg catcggcaaa
    28441 gcctttgtcc atatcgccaa tatgaccatg aatactggcg gcgatatttt tgcgagggcg
    28501 agaaccgatc gggaagttga tgatcatatg ctcgccgcgc tgggctgcat ggctgttatc
    28561 gtcttccaga gtatctggcg caccagcaac atacaccacc ggttcatcgt gcacgacagg
    28621 cgcatcttcc gccattgctt cgtcgatcga cattaccggc ttaagcactt catattcaac
    28681 gtcgatgagc ttcaatgctt cgagcgcaat ttcttcactt tctgcgacga ccgcagcaac
    28741 acgatcgccg acgtgacgca ttttcttgcc gaacatacgg cggtcaagcg gtgacggttc
    28801 cggtgcgctc tgaccacccg gggtatagta gatatccggg caattcaggt gagtaataac
    28861 gtgaacgacg cccggtaagg cttcagcttt gctgacatcc agatgagtaa tcagtgcgtg
    28921 agcgtgtggg ctacgtaaca ttttaatgac gcaggcgtca gccgttacgc ggtcttcaac
    28981 atagcagggt ttcgcctgca ccattttcgc ggcatcagtt ttaggataat gcttgccaat
    29041 gacttcaagg tcgtcacgga aagtcggagc gatatcaatg gtggcctgcg gattattttt
    29101 acgtgcaacc gccagttcaa tgacctgata gtattgttgc cagcctgcat cgcggctgaa
    29161 caaaccagaa agcgcgtcgt cgatctcttc gcgggtaggt gcggcgatgc gatcgagaag
    29221 atcggtgata atcagagctg cagctggatc gttataacca gactgcacca cgccaacatc
    29281 aaccattgcc tgttgaacca gacttaactc gttccatttg cccaaagatt ctgcggtacg
    29341 aatatctgcc ttctctaact gtgcggcaat aagcaaggac gcgttaacga tattaccgtt
    29401 aaagattatt gcgtcagaac cggcaaaccc gaagccatca tcactgttgc gtacagagtg
    29461 cattcccatg ttaaacaaca gcttttgcac gttttcgcct ggattaacgg ttagctcctg
    29521 aggcgcgcca tttaaagtaa agtggatgat catacggctt cctccccagc ctgttggcag
    29581 tcggcataca ggtcggctac cagaactccc gtgatatagc gtttataggc cacgctgccc
    29641 cgcaaatctt cctgcgggaa aatggcgttg gcgacagcct gttcaagtgc attgccttcc
    29701 agattttgtt tttcgacatc atgcagacgc agtgctttac tggcgacgcc atccagcgca
    29761 attcgcatac cgtcgtggtc tgtcattgca acggctgccg tcacgacggt taaacctgcc
    29821 tgagaacggc taattttgcg ggtcgcacag gtgcgatacg gatctttaat gataatttcg
    29881 gttagcaggc gatcgcatgg gcaggccagg tagtcctcga ttgacagcgt ttcgccgttg
    29941 ccaaaaacca gttcagcatc cagtgccaac aggacgggaa gcagcaccga ctcttcctgg
    30001 cgggcggcga tttcaccacc aatggtcgac tgattacgaa catggcgtga gtaaacaaaa
    30061 ccgagggctt cacgcagcgc tgcaggaata aatcgcgcat cacgcagtgg ctgcaagcga
    30121 gacattgcgc caatccgcag tgcaccgtta tcccagtcaa cccagtccag ttccagatcc
    30181 tgtaaggaaa tggcaatctt tttatcggta cgggttggtg tagcattgag tttgctgccc
    30241 ccggcgaacc agacggcttc atcctggtag cggcgcttca gttccagcgc ctgttcgaca
    30301 gagtcgggcc tgaaaaattg ttcaatcatc ttctttcctt atttaaccct cttccctgcg
    30361 ggaagagggc atctgtcatt tatgccagcg catccatccg acgccacata ctggcagcgg
    30421 cttttctggc ttgtgcataa atggaatcgc aatcgaagtt aaactgacgg tcttcataga
    30481 ccatcacacc attgaccatc acgctgtgaa cgctgcctga gcccataccg aaagcgatat
    30541 gcccggcgat attgtctgcc agcagcggcg tcggtgagtt gtaatcgcaa atggttaaat
    30601 cagctttgta accggcttcc agaagtccaa atttcgcgcc aaagttgcgg ctcatcagtt
    30661 cgttaccgtt agtcagggct ttggcaaaac tgtcaggcca cagcggacca cccgcatcgc
    30721 gatgtttaaa gaaggcaaat ttcatctctt caaacatgtc cgaaccaatg ccgtccgttc
    30781 ccaacgccag attgcggatg tcgctaaggt gatggttgta gccgacatgg ttgttcatgt
    30841 ttgaacgggc gttatgcacc aggaacgcat cgcgctgatt gagtagggcg atgtcatctt
    30901 tcgacaagta cagcccatga gcgaccagcg ttttgctatc gatgagatcg aattgcgcta
    30961 gtcgtgccag caggtctttg ccgtaccagt ggtgactgta ggaaacgtcg taaaggtctt
    31021 ccgcagcgtg aatatgcaaa ccacggcctg tggctttcac ggcttcacgt agcatctcca
    31081 gaccggcatc tggcacggta aacggcgcgt gagcaccgat atgtgcttcc accagatacg
    31141 gctcggttgc tgctttcttc gcctcatcaa tctgacgggc gaagcggatg ttttcttcta
    31201 caccttcctg caactctttg atgccgttgt tacggtcagt agtttcaaaa caggtcatcg
    31261 cgcgcaggcc aacttttaaa aatgcgtcgc gcaatgtgga gagcgacccg ccgatatacg
    31321 ccggagaggc atggtgatcg ataaccgatg tacatccgct cttaatcgct tccagggaac
    31381 aaatcagccc gctgtaatag agcgactctt catcaagggc gcgatcgagc cgccaccaga
    31441 gatttttcag cgttgagatg aaatccgggc agggggcgat gtttgccata attccgcggg
    31501 aaagccccga gtaaaaatgg ttgtgcgagc agacaattcc cggcatcaca atccgaccat
    31561 gcatctcttt gtagctggca tcggggtagc gttgcgtcag ggcatcgccg atagcgacaa
    31621 tcacatcatt ttcgatagcg atatcaacgc cttcctgcac tttcgccggg tgtaactgca
    31681 ctgcggtgac attcttcaga atcaacatga ttacacctcc acgcggccca gcagataatg
    31741 atgatgctgg tggacatggc tgatgatgcg gcacatatcg ttcagctccg gtggtacgtt
    31801 gttgaactga ccttcgctgt cgatgtttaa cacccagctt tggttattca gtcgtactcg
    31861 tacccggcaa tcttccacaa ggaagcctgg gttgctgctg ttatcaaagt cttgcgccag
    31921 gctgaaaacg gtgattttgt ctttgtatgg tttaccgttc cacggacaga actgagcgca
    31981 gttgccgcat tcgttacagt aagcgtcgag gtgcagcgtc tggaatctat tctggaagcc
    32041 tgggaccgca atggagacgt tggcgcggtt cgggcagaca tccacacact tgctgcaaac
    32101 gtagttacat tcgaggcagc gagcggcttc ctgggcgaca aacgcgtcac gatcgtcact
    32161 gttcaccaga gtgatagaga tatcgccttt acgttgatag atttccgccg gattgacgtt
    32221 gttccagtat ttatcgttct ggtgggaacg gatattttcc cgactaagga tggcatcggt
    32281 cgcccgacgc gcggttccga cagcagcgac aatggaggat ggtccgcgct gtacgtcgcc
    32341 gatcataaag acgtcagtca gacgagtttc gccattgtgg tcgacgtctg gccagccgtt
    32401 tttgtccagc ggcacgccca tcgcattcag ggcttcagta tcctgctgtt caccaatggc
    32461 ggtgatcagg ctgtctacat gcagtgttac tgtttcgttg gtttcaaccg gacggcgacg
    32521 gcctttctca tcgggttcgc caagcgacat aacgcgcaag gttaaggtgc catcagcatc
    32581 gaaacgttcc ggattattca ggaaacggaa ctccacgccg tcgtgcaacg cttcttcata
    32641 ctcttcgcgc catgcgggca tctcttgcag tgaacgacgg taaacgatcg ttgctttttc
    32701 tacgcctgga acgcgtaacg ccgcacgagc gcagtccatt gcggtgttac ccgccccgac
    32761 aacgaccaca tgtttgccca gcttgagcgc tgtacccttg ttgtattcac gcaggaaggg
    32821 gagtgatttc caaacatttt ggttgtcacc cgccagtttc acaccgctat ttttatcagt
    32881 gccggtggca atcagaacat agtggaagcc ctgatttttt aactgttcaa cggtcaaatc
    32941 gggtgagcag ccatactcaa atttcacgcc gtgatcggca acaaaatcga tatcgtgctg
    33001 aattaactca gccggaattc ggaactgagg aatgatattt ttcaccacgc cgcccgcatt
    33061 ggcttcgcgt tcaaacagcg taaccggatg gcccgctctg gcaaggaagt aacctgctgc
    33121 cagacccgcc ggacctgcac caatcacggc aaccggatgg cgtgaaccag aaccggctgg
    33181 tttgtgccag cgttgcttat attcatccca acctttttcc agcgcgactt ttttcagttc
    33241 gcggatattc agcgcactgt cgtaatccag gcgggtacag ttgtattggc actggtgatc
    33301 gcaaatatga ccggtaatgg cgggcagagc gttgcgttgg tagatgagtt ccagcgcgtc
    33361 ggcatagcgg tgttcgccaa gcagacggat gtattccgga atatcttgct tgatagcgca
    33421 ggcagtaaca cagggggcaa cgtagcaatc ggtcagcggc aggtcttctg ccacttcaat
    33481 acgctcttct ggcttccagt gtttctgggt gtattccatg gttaacgcat ctgctgccag
    33541 tctgttcagt cgttcgacgt caacatggtc aagtccccag gcgtcggagc cttccagctc
    33601 gcgcatgcag gcacttaagc gcaaatagcc gccaggtttc agcaggtcgg ttgccatagt
    33661 aatagggcga atacctgtat caaaaatatc gcggatagtc agctgactgg caccaccgga
    33721 ataagaaatg ggcagtttgc cgtcaaaggc gcgagagaga actgctgcaa cattgatgga
    33781 gagcgggaac agcgcacggc ctgacatata catctcttca ccaggcagtg cgcctttatt
    33841 gttgatggtg ccgagagtgt tagtcagttt tacgccaaag ccgagtgatt tttcttttgc
    33901 cagtgccatc aggcgttcca gcatttccag tgcttgcgtc agcttgaggt cgtgatcaaa
    33961 tgactcttct tttaagccta tgtaaccgaa accgcagaca tcgaggatct cacgaacacg
    34021 cgcgtatccc agtaaggtcg ggttaagttt cacaaaggtg ttgagccctt tttcttccag
    34081 catgtagcgg caaatggctt cgatttcatg cggaggacag ccgtgcatgg tggagagggt
    34141 gacgccctgc accatactgg tggggatgcg agcgggtaag gcttgcaagc tttcgcgttt
    34201 ttcctgcaat ccgtggcggg ctaaaaatgc gtcatcctgg agtaatttat tcagcgtatc
    34261 gcgatattgc gcgaatttcg gatggtcaga tgcgtccatc atattgtcga tgaactgttg
    34321 cattggcggt tgcttaatac cttcgaggtt gtaaccgacg ctcatattaa agatgaacga
    34381 tttaccggaa tcagaaggct ggaacatcgc ttcgagaagg tgcagggcaa accaggcttt
    34441 gaggtattca tcccaggctt taagcagggt aaattcggta gaccattcgg tgttaaagca
    34501 ctcgtcttcg gcatcgatac agggcttttc cagctccagg cggtcaagaa tttggacggt
    34561 ttttagttcg atgaagcgtc cgccagtcag ccaggaagtg acaatatttt gcgcgagctg
    34621 cgtgtgcggg ccagcggcag ggccgacggg agtggcacag gtttcaccga agacgctaac
    34681 agttttacct tttacaggtg agtaaaactg ttgctcggga ataccaaaga ttgagcgttg
    34741 ttgttggtat tcatcaaata tgcgcgtcaa aagttcctca aacggaatgg gacgcataat
    34801 atcccccata accctctcct tagcttatac agatatcatt gagtgaccgg tgttgagaaa
    34861 tccggtggtt tagggggata gagcaacaat cgcaccactc ttgccaggtt tggcgggaaa
    34921 tataaaaaaa tgtgaggcgg ctcgaaactt aaaggctttt tgtgaatgaa ctcacaggtg
    34981 ccaaatgaga attaaaatca ctcctgaaaa ttattattgt cagcccattt atcacttttt
    35041 ctcttttctc aaaatcagtg attccttatc attatgataa agagtgctgg agagattaaa
    35101 tatttgaacg caatcgttta actacatttt ctaaatttta gtataccttt tttgcgctgt
    35161 aataaagtcg tccggagtat caatatctaa aattatttct gcattttcta tctcgacgga
    35221 ataatgagct cccataagga gtgcctgacg catattggtg acattgggtc gttggattgc
    35281 ctgcatcaag catggttttg acactaaaat cggatggccg gggatgccat tatggagtgg
    35341 cagtattgcg ccatcatttc gtaacgacca gatttttcta aaaatatcga tggtgagggt
    35401 tggcatgtca ccgtgggtga gaaaacaatg ttctgtttgc actgcgggta ctgcggcctt
    35461 tactgatgtt agtaaaccct gcgcataatc tgggttgtgg ataatagtga tattgctctg
    35521 gttcgcataa cgttcgtgca gttcattacc acgatagccg gtgactaaaa taattcggct
    35581 acaaaactgc aacgcatttt tgatacttgt atcaagaatt gtcccctgtt gccagggtaa
    35641 catcattttc cattgcccca ttcttgatga taatccggca gcggtaatta tacagtcgat
    35701 ggctgacata gtggttccat tatttatagg ggctaataac gcctgataat tgcgttactt
    35761 cctgatggat taagttgggg ataatttatc ccagagaggt cataaagact cgtgaaaagt
    35821 atagttgacc catcggcatt agtcattgat ttaggtgcgc agaaacgtcc cactgtgatt
    35881 tctgttgttg gtgctggggg gaaaaccagc ttgctttttt ggctggcaga actgctccag
    35941 gctagcggca ggcgtgtgtt aatcacgacg acgacacata tgtttatgcc aacgtctcac
    36001 tggcccgtgg ttttctgtcg tgatcccgcc atgcttcctc atgcgtctct tacatccccc
    36061 atttcatttt gttttcacag ctggaaagcg aaccagggaa aagtgcaggg atttacgcca
    36121 gaagcgattg atgcactggt gcaacgacca gagtgtgacg taattctcat tgaagcagat
    36181 ggctcgcgtg gaatgccgtt aaaagcgcct gatgagcacg aaccttgcat acccaaaagc
    36241 agttgctgcg tgattgctgt gatgggagga catactttgg gcgcgaaagt gagcacagaa
    36301 aatgtccatc gctggtcgca gtttgctgat attaccgggt taacacctga tgcaaccttg
    36361 caactgagcg atctcgttgc gctggttcgc caccctcagg gggcgtttaa aaacgtaccg
    36421 caaggttgtc ggcgagtctg gttcattaac cgtttttctc aatgtgagaa tgcgattgca
    36481 caaagcgagc tccttcaacc gctgcaacaa cacgacgtag aggcaatctg gctgggcgat
    36541 atacaagaac atcctgcaat cgcgcgcaga tttgtgaatt agcggcccgg gtattccggg
    36601 ttactgaaag gatatccatt gaggttggta tgaatatttt cacagaggct gcaaaactcg
    36661 aagagcaaaa ttgtccgttt gcgatggcgc aaattgttga tagccgaggc tcgactcccc
    36721 gccattctgc acaaatgtta gtgcgcgccg atggttctat cgtcggtaca attggtggcg
    36781 gaatggttga gcggaaggtg attgaagagt cacttcaggc attgcaggaa cgtaagccgc
    36841 gattattcca tggacgtatg gctcgtaacg gtgcggatgc tgtcgggtca gattgtggag
    36901 gtgcaatgtc agtatttatc agcgtccatg gtatgcgtcc acgtctggtg ttgattggcg
    36961 cggggcatgt caaccgggcg atagcccaga gtgcggcgct attaggattt gatattgccg
    37021 ttgccgatat ttatcgcgaa agcctcaatc ctgaactatt ccctccatca accacgcttc
    37081 tccatgctga gtcgtttggt gcagcagtgg aagcactgga tattcgccct gataattttg
    37141 tcctgattgc cacgaataat caggatcgtg aagccctcga caaactcatt gaacagccca
    37201 ttgcatggtt ggggttgctg gcaagtcgcc gcaaggttca gcttttcctg cgtcaattgc
    37261 gtgagaaagg cgtggctgaa gaacatattg cccgtttaca tgcgcccgtt ggttacaaca
    37321 taggtgcgga aacgccgcag gagatcgcca tcagcgtgct ggcagaaata ttacaggtga
    37381 aaaataacgc gccgggtggg ctgatgatga aaccttctca tccttccgga caccagctgg
    37441 tggtgattcg cggtgcggga gatatcgcca gtggtgtggc gctacgtctg tatcatgcgg
    37501 gttttaaagt gatcatgctg gaagtggaaa aaccgacagt gattcgttgt accgtggcgt
    37561 ttgcccaggc cgtgttcgat ggcgaaatga cggtcgaagg cgttactgct cgcctggcaa
    37621 ccagtactgc ggaagcgatg aaacttaccg aacgcggatt catccctgtg atggcagatc
    37681 ccgcctgttc attgcttgat gaactgaaac cgctttgcgt ggtggacgct attctggcga
    37741 aacagaattt gggaacgcgg gcagatatgg caccagtaac aatcgcgctt gggccgggct
    37801 ttactgcagg gaaggattgt catgcggtaa ttgaaacaaa tcgcgggcac tggctcggtc
    37861 aggtgattta ctctggttgt gcgcaggaga ataccggtgt tcctggcaat attatggggc
    37921 ataccacccg acgggtgatc cgtgctcctg ctgcaggcat tatgcgatcc aacgtgaaat
    37981 taggcgatct ggtgaaagag ggcgatgtga ttgcctggat tggtgagcat gaaattaaag
    38041 caccgttgac ggggatggtg cgtggcttgt tgaacgacgg cctggcagtg gtcggtggtt
    38101 ttaaaattgg tgatatcgat cctcgtggtg aaacggctga tttcaccagc gtttctgata
    38161 aagcccgggc gattggcggc ggcgtacttg aggcgttaat gatgttgatg catcagggcg
    38221 tgaaagcgac aaaagaagtg ctggaagtgg cttaaatgaa aatggccgga tggtattacc
    38281 cacccggcct taaaattact gacaaatcac cgttccggtt ttgccttcaa tcccctcttt
    38341 cgctttgctc aatacggtaa tcagtgcttc acgacctgcg cgagagcgag caaacgaggc
    38401 ggcagcttcc actttaggca gcatagaacc tttcgcaaaa tgaccttctt cgataaagcg
    38461 ttcagcatca cttaatgaca ggcgatcgag ccactgttca ttctctttac cgaagttaat
    38521 ggccactttt tctaccgcag tcagaatgat cagcatatcg gcatcaatca tttctgctaa
    38581 acgggcgctg gcccagtctt tatcgataac cgcgctggca ccacgcagat ggttaccttc
    38641 acgaataaca ggaataccac caccgccaac ggtgatcacc acctggcctg catctagcag
    38701 agctttaacc gtttcttttt caataatatc aaccggtttt ggcgaggcaa caacacggcg
    38761 atagccgcga ccagcatctt ctttcagagt gtaaccctgt tttgtcagtt gctctgcttc
    38821 ttgctcggta aagaacgaac cgatcggctt ggtcgggttg aggaatgccg gatcgttagc
    38881 gtcgacttcg acctgagtaa ccagcgttgc taccggttta ttaatgcccc gagaaagcag
    38941 ttcttctctt aaggcgtttt gtagatcgta accaatataa ccctggctta aggcaacgca
    39001 gacagacatc ggcagcattg gcgagtgcgc ttcagttttt gcagcagctt caaaggcctg
    39061 gttaatcatg cccacctgcg gcccgttacc atgagtgacg ataacttcat gtccttgggc
    39121 aattaaatca acaattgcct gagaagtaat ttttaccgct ttcatttgtc cggccaggtc
    39181 atcgcccagc gcattcccgc ccagggcgag aacaattttc ttactcattt ttcctcactc
    39241 atcattcttg attgtgtata gatccctgac agtggttaga gcacgggagg gacaaacggt
    39301 ttgcgtcgca ggaaacgacc tcgcccggct ttgcctgtaa aggtgccgtc acagaaaatc
    39361 gtttcaccac gggataaggt tctgacaatc gcgccctgac aggtaaaacc ctcccatggc
    39421 gagtagtcgg cgttgtcgtg gagatggcga tgctgaattt gttggctctg acgtgggtcg
    39481 ataatcacca cgtcgccatc ggaaccgggc gctaataatc ctttttgcgg ccacaggcca
    39541 aacagcctgg cgggcattgc gctggttaat tcaacaaagc gttccggtgt tatacgtccc
    39601 gtcatcacgc cactggaaaa cagtaactgc atgcggtttt ccacaccggg taagccattt
    39661 gggcagcgac tgaaatcgcc tttagaaatt tgcaggcgtt gagccatcga gaaggtgcag
    39721 tgatcggtcg ccaccacgtc aatcgcacca tcgctgatgc cacaccacag tttgtcctgc
    39781 tcgcgtacgt tacgcagcgg tgggctaaga atgaacttca tgccgtcttc tgtatcgtaa
    39841 ctgcgttcgt ccaacaggag atattgtggg caggtttcaa cccagactgg ctggtgattc
    39901 gcacgggcaa gacgcagata atccagacct aagccgttag acaggtgcac gatatagagc
    39961 ggggcgttac cggcaatttg tgccaggtta atcatgcggg cgatggcttc cgcttcgcat
    40021 tccagagggc gactcaaggc atgatagcgc ggcgcggtta accctgcggc gataaattcc
    40081 gcccgcttgc tggcgatagc cgcatcattt tccgggtgca cggtggtcag cgcgccggat
    40141 tcatgcagac ggcgtaatgc ctgcaaaacc tcgtcatcgt tgagtttgta ttgataggtt
    40201 aaatagagtt taaaactgct cagtccttcc tcgaccatca tcgggatttc gtcgaggatt
    40261 gcgtgattaa tgtgctggat cacaccgtga aagctgtaat cgatgaccgc tttatgggcg
    40321 gcataaccac gataaacctc cagttgatgg cgtaaccgac agccgtttgg gccaaatccc
    40381 atatggtcaa taatggttgt tgtaccgcca cacgcagctg cgcgggtacc ggtaaaaaaa
    40441 tcatcacaac tgcgcgcgat gccgacatca atattgaaat gcgtatggac atccacgccg
    40501 ccagggaaaa cgtaacagcc agtggcatca atttcttcat acgggagctg cggcgaaata
    40561 ttgttgccca actggcgaac aatcccgctt tcaatcagca aatcctgttt ggcttgtcca
    40621 tctgcgttaa cgacagtgcc gtttttgatc aatacgcgca tagcaaactc caaaaaccct
    40681 ccggcgaacc ggaggggaat aagtgaagag taattactct gttgccagcc agcttaacgg
    40741 gattgcagcg tacatcgcgg cacaggtcac caggtgagat ttccaggttt tttcgttagg
    40801 tgcatgcgct tcaggttctt tacccgggcc aaagccgatc accggaatgc cgtgacggcc
    40861 catgatagat acgccgttag ttgagaaggt ccacttatca acaaccggcg ctttgccaaa
    40921 cagcccttcg taggcattca ccagtgcttt aacggtgaag tgatcttctt ccactttcca
    40981 ggtcgggaag tagcattcgg ttgggtaaac caggccagtc caggacggac ggtcgtagtt
    41041 gtacatagaa acaaccgcgt tagctttctg tactgcaggc agggcgcgga tttcgtccag
    41101 cgcgccttcc caggtttcgc cccaggtcag acggcggtca atagagacgg cgcagctatc
    41161 tgctacagcg caacggcttg gggatgtgaa gaagatttca gaaacggtga gggtgccttt
    41221 gccgaggaat tcgtcataac ccagacgttg ggagagttct tgtaattcgc caagaatcgg
    41281 acccattttg aaaatggcgt tgtcaccgcg ttctggcgca gaaccgtggc agctaacacc
    41341 ctgaacatca atacgaattt ccatacgacc gcgctgacca cggtatatct ggcagtcggt
    41401 tggttcggta ctgaccacaa attccgggcg aatgccggat tgttcaataa tgtactgcca
    41461 gcacagaccg tcgcagtctt cttcctgcac agtaccggta accagcaggg tatattcatc
    41521 ttccagaccg aggtctttaa tgattttacc ggcataaacc atagatgcca tgccgccttc
    41581 ctggtcggaa gtaccgcgac caccgattag ctcatcagtt tccatgcctt cgtacggatc
    41641 gaagtcccag tttttgatgt tgccaatgcc gacggtatcg atatgagcgt ccattgccac
    41701 cagacgcggg ccgtggccga tataaccgag aacgttgccc atcgggtcga tttcaacttt
    41761 atcgaagccg actttttcca tctcttcttt aatacgatgt actacgcgtt tttcgtcgca
    41821 gctttcactg ggaatagcaa ccatgtcgcg caggaagcga gtcatgtccg cctggtaatc
    41881 ttttgctttt tcaagaatca gtttgaatgg aatattctta gccattattt ttctccagat
    41941 tttttactcc ccgttttgca gggagtagtt ccaatagagt gattaaggtg ctacagcgtg
    42001 tttgccttcc cagacaactt cgcggtagtg cttcacgtcg gtgtcgcctt cagtgctgat
    42061 aaccagcacc acggcatctt tgttcagcgc cagtttttcc atcaggcttt gacgttgcgg
    42121 gtgataatga accgctgcga gaacgcccaa accgacagcg ccggattcac cggagatgat
    42181 gcgcgggtcg ttgccgtacg gattacccag cacgcgcata cctaatgcgg caacgctgtc
    42241 ctggcaggag atgaattggg tggcgcagtt acgtaggatt tcccagccca gcgggttagg
    42301 ttcgccacag gccaggcctg ccatgatggt ggccatatca ccgccaacgt tgacgatgtc
    42361 gcctttgacg ccggagcgat aaatacagtc agctttgtca ggttcaacaa taatgctgtg
    42421 cagattttgc gggctataga cgtcgaccag ataacccagc acaccaccgg ccattgctcc
    42481 gacaccggct tgcagcagaa cgtgcgtcgg ggttacgccc atttcacgca tttgctcgac
    42541 ggcttcatct gccagggttg cgtagccttg catgatccag gttgggattt tggtgtaacc
    42601 ttcccatgcc gtgtcctgta ccacttccca gccgtgctgc tgcgcgtgtt gcatggtcag
    42661 gcgaacggta tcgtcatagt tcatatctgt gacgatgcac tcggcaccga ggttcagaat
    42721 ggcgtcaacg cgttcctgag cagaaccttt cggcatgtaa atcaccgcat tctgtccgag
    42781 ttgctgcgct gcccacgcca caccgcgccc gtggttgccg tcggtggtgg tcgcgaaagt
    42841 cattttttcg ccgatggcat ttttcaggtg ctcaaatgac agcgtttcga tatcaagatg
    42901 atatttttca cacaataatt gagcgatagc gtacgcaccg ccgagcattt tgaaggcgtt
    42961 cagaccgaat cgttttgatt cgtctttgac aagaattttc ttcaccccaa aaaggtttgc
    43021 gagatcgtcc agcgcacaaa gcggtgttgg gcgataacca gctattttct ggtggaactg
    43081 gcgcgccagt ttggcctggc tttgcgaaaa gagcggtgat gtttcgccgt tgaaaaattt
    43141 gttatcggcg atatcaatct tcaatgagaa aacggacata tcctatcctt ttggcctctg
    43201 gaaatagagg gccgaccagc tgatcggccc gaggggttat ttcacgcgtt cttgcgcttc
    43261 tttcagcagt tgctcgagca gtgcacctgg tttggcgtat ttacgggaca ggatcatcgc
    43321 ggcgatgata taaggcttcc agctggcttc tttgtaggta gcgatacggt atttttcgaa
    43381 tacgccttca gtcacttccc cttctttgca ggatacgccg ctgatatcag ctggcaggca
    43441 gtgcatgtac agggcttcgc catcacgggt cagttccatc atctcttcag tacaatgcca
    43501 gtctttgtgt tgcgcgttct gtgccagaca ctgtttttcc agtgctttta agccttcgtg
    43561 atcgttagcg cgcagcaatt cggtacgctg ttccatcact ttgtaaggtg cccatgactt
    43621 cggataaacg atgtctgcat ctttgaacgc ttcttccatg ctggtgacct gacggaagct
    43681 accgccagat gctttagcat tgtttttcgc cacttcaacc acatccggga tcaggtcgta
    43741 gccttccgga tgggccaggg tgacatccat accgaagcga gtcatcagac cgatgatgcc
    43801 ttgtggtaca gagagcggtt tgccatagct tggagagtag gcccaggtca tggcgatttt
    43861 tttacctttc aggttttcca gtgaaccaaa gtgttcacgt aaccacgcga ggtcagccat
    43921 tgactgagtc gggtggtcaa tatcgcattg caggttcact aaagccggac gctgtggcag
    43981 tacaccctgc ttgtaaccgt catcaagtgc agcgccaact tcacgcatat aggcgttgcc
    44041 tgcgcccaga tacatatcgt cgcgaatacc aatagcgtcg gcgcagaagg agatcatatt
    44101 ggcggtttca cgcacggttt cgccgtgagc gatttgtgat ttgccttcgt cgagatcttg
    44161 ttgtgcaagg ccgagcaggt taagcgcgga agcataagag aagcgggtac gggtggagtt
    44221 gtcgcggaat acggaaatac ctaatccact attaaagact ttggttgaga tgttttcagc
    44281 acgcagtgct tttaatgctg cggcaacgtc cagtacttgt ttcagttcat ctggcgtctg
    44341 ttcccacgtt aacaaaaaat ctttctcgtg aaggtgagag gtcagcgaat tgatatcctt
    44401 aatcagctca ttaacagttt tcatcataaa gtcccggtag tggaatgaca ggcaaagtgg
    44461 tttatttcac tgtgacttgc caaagtgatt ggtgttttca cagctatacc tgggactaac
    44521 aataagtgtg ccagaattat ccacggggct gcaaaagact aatttgacgg ataatgtgtg
    44581 gggttgatca caaattgatg ccagcttccg gttgtttgta aaatacgaaa acgtttggct
    44641 ttttttatcg cgttatttat ttgctgctaa gtatcagaaa taagataaag ggacaggcta
    44701 tctctcttca gtttgataaa ctcaccgccg taattctcat attgatatag aaatgtgata
    44761 tctcttccat aatttcatta gaaaatgata aaaatcttat gtttttatca aagccaattt
    44821 cgcctgagtt aatatcatca tgatctgtga accatcaacg tcttcaatga tcgactttta
    44881 agtaagtctg gaaggtaaag ctatggagct tgctactacg cagtcagtat tgatgcaaat
    44941 tcaaccgaca attcagcgtt ttgccagaat gcttgccagc gttttgcagc ttgaggttga
    45001 gatcgttgat gaaaacttgt gtcgcgtcgc cggaacgggc gcgtatggga agtttcttgg
    45061 tcgccagttg agcggcaact cacgcctgct ccgccacgtc ctggaaacga aaactgaaaa
    45121 agttgtgaca cagtctcgct tcgatcccct ttgcgaaggt tgcgatagta aagaaaattg
    45181 ccgtgaaaaa gcatttctgg gtacgcctgt cattttacag gatcgttgtg ttggggtgat
    45241 aagtttgatt gccgttaccc acgagcaaca agagcatatc agtgataatt tacgcgaatt
    45301 ttctgattat gttcgccata tatccaccat ttttgtttcg aaacttctgg aggatcaggg
    45361 gccaggagat aacatcagta aaatattcgc gaccatgatc gataatatgg atcagggcgt
    45421 attagttgtt gatgatgaaa gtcgggttca gtttgttaat cagactgcct taaaaacact
    45481 tggtgttgta caaaataata ttattgggaa acctatccgt ttcagaccat taacatttga
    45541 gagtaatttt actcatggac atatgcagca tattgtttcg tgggacgata aaagtgaatt
    45601 aatcattggt caattgcata acattcaggg ccgacaatta tttttaatgg catttcacca
    45661 atcgcatacc agtttttctg tagcaaatgc acctgatgaa ccacatattg aacaattggt
    45721 tggcgagtgc cgtgttatgc ggcaattaaa acgactcatt agccgtattg cacccagccc
    45781 atccagcgtt atggtggttg gtgaaagcgg cacgggtaaa gaagtcgtcg cccgagcaat
    45841 ccataagttg agcggaagac ggaataaacc ctttattgct atcaactgtg ccgcgattcc
    45901 ggagcagctt ctggaaagcg aactgttcgg ttatgttaaa ggcgcattta ctggcgcttc
    45961 tgccaacggt aaaacagggt tgattcaggc ggcgaatacg ggcacgctgt ttctcgatga
    46021 aataggtgat atgccattaa tgttgcaggc taaattactg cgcgctattg aggcgcgtga
    46081 aattctaccg attggtgcca gtagcccaat acaagtcgac attcgcatca tttctgcaac
    46141 taatcagaat ttggcccagt tcattgccga aggtaaattc cgcgaagatc tcttctaccg
    46201 acttaatgtt atcccgataa ctctgccacc gctgcgtgaa cgtcaggaag atattgaact
    46261 attggtgcat tactttttac atctgcatac ccgtcgtctg ggatcggttt atcctggcat
    46321 tgctcccgat gtcgtcgaaa tattgcgtaa gcatcgttgg cccggaaacc tgcgcgagtt
    46381 aagcaatttg atggaatatc tggttaacgt agttccttca ggtgaagtta tcgacagcac
    46441 gctattgccg ccaaatctgc tgaataatgg cacaacggag caaagtgatg taacagaggt
    46501 cagtgaggcg cacctgtcac tcgatgatgc gggcggcacg gcgctggagg agatggaaaa
    46561 gcaaatgatc cgcgaggcgc tttcacgtca taacagcaag aagcaagttg ctgatgaact
    46621 gggcatcggc attgctacgc tctatcgcaa gattaagaaa tatgagttgt taaacacata
    46681 acctcagtga atccggcctg aattcaggcc ggatatcctt ttacttcgtt ttctcgcaat
    46741 ccagaactgt atttacaatc atctgatacc ccgtgcagcg acaaagattt cccgccagtc
    46801 cgcgacgaat ttccgtaatg gttaatggct tctcgcgtgg tttcgccagc attgccgtgg
    46861 tagccataat caggccaggc gtacaaaacc cgcactgcac tgcgccggat ttcgcataag
    46921 cctgctgaac atgagaaagt tttccgcctt tcgcttcacc ttccagcgtg cggatctctt
    46981 ttccttcagc ccaggcggca aggtataagc aactgtctat tgctgtgccg tcgaccaaca
    47041 ccgtacaggc accacattca cccacgcagc acccttgttt gacacttagc agtccttgtt
    47101 cgcggagtaa ttccgagagc ggcgtgcctg gtgcggcgtg aagctgaaaa ggcatcccgt
    47161 taatggtgca ttcgatggta attgtttcgc tgtgattcat tgcaattttc cccccgccgc
    47221 ggcgacggct tcgctaatca cttttttggt catcgtctgg atgagatgca gacgaaactc
    47281 tttactggcc cgccatgaag aacgcggggc gacatcttgc aggacagatt cgctgatagc
    47341 ttccagcgtt tgcaggttta atggcgcatt ttgtgcagtc tgttcggcat gttggcagcg
    47401 aatcggcgtt ggcgcggcaa caccaaatgc caggcgtaat tcgctgaaat tgccgttatc
    47461 cagtcggcaa tgtgcggcgc agccaatcgt tgaaatatcc attgcgtcgc gcatggcata
    47521 tttaaaatgc gcgctgcccg cgtgttcttt cggctgtggc ggaaaatgaa aggcgacgag
    47581 gatttcgtca tgctcaagag acactttgcc cggcccggtg tgaaagccat taatcgggac
    47641 gaaacgaaca ccgcgcgggg agtggatctc cagtttcgcg tcataaatta gcgttggcgt
    47701 ggcagaatct gcgctggtgg caccgttgca aatatttcca ccgtaggtag cgacgttacg
    47761 gatctgcggt ccagcaatgg acgtggccgc agcacataac gccgggagat gacgttgagt
    47821 tataggatct tctattagct gggtaaatgt cgttgcagag ccgatacgta gcgagccatc
    47881 ttccgccagc gtaattcccc gcagctccgc cagattatga atatcaacaa tatggcgata
    47941 acggtcattg tgatggtgga gctgaatcag tacgtcagtg ccaccggcga gcagtttggc
    48001 ctgcgggttg tcagccagca ggttgatggc atcggcaagg gttgctgcgc gatggtaaga
    48061 agcaaaatca aacatgatgt tatcctcaaa tcaatcctgc cagatggaac tcttcatata
    48121 accgttttgg cgtcagcggc agtgtattga ttgcaacacc ggtagccatc ttcaccgcgt
    48181 tacgaatagc agcggcaaca ggaattattg gtggctcacc cagtgactta tgtccgtatg
    48241 cggattgcgg ctcattgatt tcgacgaacg cgctttccag ttgtggcaga tccggcatgg
    48301 tcggcatttt gtaatccagc agattggggt tacggaccac gccgctttta gcatcgatga
    48361 tcatctcttc aaatagcgcc cagccaatgc ccattcccat tccgccgtgt acctgacctt
    48421 ctgccagcag tggattaaga atatgccctg aatcatgaac gttgaggatg cggttgatgg
    48481 tgactttgca cagcgcaata tcgaccgtca gatcaacaaa ggtacagcca aacgccggtg
    48541 ggttagtggt ggttttgatg gagctttcag cagagagctg cccgccgcgt tcagggtggt
    48601 agaaagcgtc catcgccaaa tcttttaacg acattaacgg ctcttccggt cgttcaacca
    48661 gcacgatatg gccttttatc agggtcagat tcatcgctga ctgatgtagc atgactgcgg
    48721 cgtgagcgat gattttctct tttaataata gtgccgcact gcgcagcgca ggcgcggcaa
    48781 catagctctg gcgtgaggca aatgcgccgg gatcgaacgg cgtaacgtcg gtatcttgag
    48841 ttgaaataac gcgaacgtcg ctgaccggaa cccccacggt ttctgccacc atttgcgaga
    48901 agacggtgtc ggcaccctga ccgatttccg tcgcgccgct ttgcacgttg atggttccat
    48961 cctgattcat cagaaggcgc gcgcctgcta tttctacgcc gacaggccag gtgttagagg
    49021 tgtagctaaa acaggcgacg ccaacgccgc ggcgcaaatt gccttgctgg ttctggcatt
    49081 ctgcacggcg tttttcccat tcaaagattt tccggccttt ttcaagacac tccggcaacc
    49141 ctgcgctgta aatacgtttg cccgtgagcg gattagcatc tccttcgcgg gcggcgttgc
    49201 gtaaacgaat ttcaacagga tcaataccta acgctgtcgc ggcgtcatca agcatagact
    49261 caacggcaaa tacgacttgt ggcgcgccat aaccacgcat cgcaccagcc gaggggaggt
    49321 tggtatagca ggtctttgaa ctgtaagcgt aggcacaacg aggataaagg taagcgactt
    49381 tattcccccc cgcagaagcg atggagtgcc cgtgagatgc ataagcgccg gtgttagaca
    49441 gaacatccag actataacct ttcaatgttc cgtcgcggtt cacgcccatt tgcccgtcaa
    49501 tggtaaaagc gtggcgggta cgggttgcga ggaaacactc ttcacggcta agggaaactt
    49561 tcaccggaat gccgccaagc ttgctggtca ggaatgccgc cattggctct tccagtacat
    49621 cctgtttatt accaaaaccg ccaccgacaa acggtttgat gactcgtacg catgaccagg
    49681 gaatatccag cgcctgacca accacgcggc gaacaatgtg cgggatctgg gtgctggaaa
    49741 cgatggtaat tcgcgagtca tcctccatcc acgccagcga tgttacgctt tccatgtgac
    49801 aatgttgaat aacgggggtc tgatagtgcc cctgtacctg gtagtcggcg gcatcgattg
    49861 tttgttggac attacccgtc gacatcgtgc tttgtttcag taaattgcca ccgttatgga
    49921 ttggtgcagc gtcttctgcc agcgccgctt ctggcgtggt gataacgggt aattcttgcc
    49981 actcaatgct gaccaattgc gccgcttttt ctgccgtgag ttcatcgcgg gccacgacga
    50041 tggcaacggc gtcaccatga tgacgaacat ggcgagttag cagtgcgcga tcggcggtat
    50101 cgcgcttgtt ttcgtcaagt gtccaggcat gcccagcagt agcaaatgga atatcaggca
    50161 catcttccca ggtaaaaatc gccagtacgc ccggcaaact tctggcttgt tcatcattaa
    50221 tacttacggc ataaccatgt gcgatagggc tacgtacata tttcgcataa cacatgcccg
    50281 ccataacata atcgtcagta tatcgtgccc gcccggtgac cttagcaatg gcattgacgc
    50341 gcatgcatga ttcacccgta gcggttgctt cccgcgcttc catagaaatc ccctcgattt
    50401 gaacatttgg ttcttatggc aaagatttag tctaagcagg caagatttac gccagaaacg
    50461 acgataagcg ttaggggagg taggaagtgt gatgacgtta aaaaaacaca gatatgatac
    50521 gaaacaggac tctgaatagg ggcagtgata cgtatcaata tgagtggcga taaaatcaat
    50581 gtgataaatt gggattatta cgcatcagga tgggaaatag ggtaaaaagt caggaagttc
    50641 gatacctcta cagcgaatat tttgctgatg atacttccct ttgccaaatg aaattaacct
    50701 ctttctctat taccacgttt ttccagaagc aagagattgg gtatcaagag gctggctgct
    50761 atgataaggc gtcttgtttt ttaagcgagg aaagattttg agtgcgggac gcctgaataa
    50821 aaaatctctg ggtatcgtga tgttgttatc ggttggactg cttttggcgg gctgttcggg
    50881 tagcaaatca tccgatacag gaacgtattc cggctccgtt tacaccgtga aacgggggga
    50941 tacgctatat cgtatttcgc gcaccacggg aaccagcgta aaagaactgg cgcgactgaa
    51001 cggcatttcc cccccttaca ccattgaagt tggtcagaaa ctaaaactgg gtggggcgaa
    51061 aagtagcagt agtacacgta aatcaaccgc caaatcaacg accaaaaccg catcggttac
    51121 accgtcatca gcggtaccga aatcttcctg gccgccagta gggcaacgtt gttggttatg
    51181 gccaacgaca gggaaagtta tcatgccgta ttcgacagca gatggcggca ataaagggat
    51241 tgatatctca gctccacggg gtacacctat ttacgccgcg ggtgcaggaa aggtggtgta
    51301 tgtgggcaac cagctgcgtg gctacggtaa tctcatcatg attaaacaca gtgaagatta
    51361 cattacggct tacgcccata atgacacgat gctggtaaat aatgggcaaa gcgtgaaggc
    51421 tgggcaaaaa atcgccacta tggggagcac ggatgcggca tctgttcgcc tgcatttcca
    51481 gattcgttac cgtgcaacgg caattgatcc gctacgttac ttgccgccgc agggcagcaa
    51541 gccaaaatgc tgatggcgaa ttaatcagca gtcagcagcg cggcacttgc taaggagagc
    51601 gtaaggttta taatgcctta cgcatctcga agcgggcgta gttcaatggt agaacaagag
    51661 cttcccaagc tctatacgag ggttcgattc ccttcgcccg ctccatgacc attcttaaag
    51721 tctactaact tactgaatcc taagggatac ggcttaaagc cttccgatag ttttactcac
    51781 tgtaaccaac actgtgacta actttgttga agtagtgagc agacctaagg tctcagtaca
    51841 tgctcaattc aagaacctac ctttatcagc gtaacggtgt cttttacatc cgtctcagga
    51901 tgaagaccac tggtcgcctg accgcttcgc ttccttctca caaccggtat aaactagcgt
    51961 cagtatcact acggactaag gacagacgta ccgctatggc tcactcacgg cacatcaagt
    52021 cagcacttaa agcaatacac gcagacaacc ctagcgcatc atatgaggaa ctacgggagc
    52081 atctgaagac cattgttgag tgggaacttg gtgtcagccg tgatgacctg aacgacccgg
    52141 agtcctacca gctttacgtt gaccagtacg acgacatcaa gtccaacctt cgggaagctg
    52201 tagcgactga gcgtctgact gtagaccagc atcgctatat caatgacgtt atcggtgtac
    52261 ttaaagcctg tcaggataga ctcaagggcg ataactctgg cctgttgtct taccttgagc
    52321 ctgagactgg cagtctaaga ccgtccgtct ccctatctgt atcatcggag cctgaagtcc
    52381 ctgaacctaa agcactgacc ctcgcgtctc tcattgagca gtacgagcag gagaacgccc
    52441 agaactggaa gccagcgacc ctaagcgaaa accgagcgtc acactctacg ctaatcgaga
    52501 tattcgacta tctggatatt caggatgtcg gcaaggcgac ccgtgcagat atgcttaggg
    52561 tccgtgaagt cctccaacag ctaccgaaga accgcaagca acgctttaag tctatgccgc
    52621 tgtctgacct gctgaaccgg gagtctaaga ctgactgtct tgatgtcgtt accatcaaca
    52681 ataaatatct tatcaagatg gctgctgtct ttaagtgggc ggtacgtaac gacctgatag
    52741 ctaagaacct gaccgaaggt cttgagctga aagtcccgca gcgtaaagcc tccgacgctc
    52801 gtgatgcttt cagtcctgag caggtagggc aactactggt cgccgctaaa gcgtactctc
    52861 agaagaccgc aggtaagcca taccattatt acgtcaccgc tctggctgcg attacaggtg
    52921 cgagacttaa cgaggtggcc cagcttcagg tcaaagacgt cagggctact gaggccggga
    52981 ccgtatatat ccatatcaac gaagacgata gcagcctacc gggtaaaagt attaagaacg
    53041 ctcatagcga ccgctgtgtc ccgctggtcg atggcgctta tggttttgta ttggctgact
    53101 ttatgtcgct tgtggaagac cgtagaaaga ctgcgggtga taatgctatg gtattcgatg
    53161 gcctcaagct gatgaagaat ggctacggtg agcaggtgag caaatggttt aacagaacgt
    53221 tgctgcctaa agtccttgct gaccgtagcg gcttagcctt tcactcattc aggcacactg
    53281 ttgcgaccca gttaaaacaa catggggtcg agttagcgta tgctcaggcg attatgggtc
    53341 atagctcagg gtctataact tacgaccgtt acgctaaaga ggtcgaggtg gatagattgg
    53401 ttaatgtgat ggcaggtgtt tataaggaga ctggattaaa tggctgagta ttatccggcg
    53461 gtgtttgaag cagaggcttt taactgtcct cactgtggtg tatatgcccg tcagttctgg
    53521 cgtaggatgt atggaagtgc gcgtgagttg gcgcaaagga caaccgagtt ccgaatgtca
    53581 acatgctctc attgcggtga tgatgcttat tggtatgacg gcaatatgat tattccggcg
    53641 gctggcaatg ttgaactacc aaatccagat atgccagata actgcaagtc cgactatatg
    53701 gaagcccgaa gcatcattaa tctgtctcct aaaggggccg ctgcactcct tcgtctttgc
    53761 cttcagaagc taatggtcca cttaggcgag ccgggagaaa acattaataa agacatccgg
    53821 tcactcgtcc agaaaggact acctgtacgc atccagcagg cggcagatat ctgtcgtatt
    53881 gtcggcaatc aggcggtcca tcccggagag attagtcttg atgatgaccc gcagttggca
    53941 cacggtctct ttaagctgct gaacattatt gtcgatgacc gaatcacgag accgaaagaa
    54001 attgaggcta tgttccagag tatgcctgaa ggtccacgtc aaggtattga gaatcaggac
    54061 agacaggcga gggagcagca gcaggccgct aacgaatgaa acccacacta taggggaagg
    54121 ggggggacta aactatcact ataggggaga gaccctaagt ccccccgggc ttaatacagc
    54181 caccgaccca cacattcatt aagtctcagg cttccggtag cggaccgatt ggaaaccatc
    54241 cgcaataagc ctagtgttaa atcaccttgg cctgagacca ttccctttaa cgtagcaatc
    54301 tctctccttt gacaggcgga gaacatcaga ccgcagcgga agtatccgtc tatgcgacca
    54361 tcggtgtgcg agattgtgaa gaaccaagac atcattgctg aggtctgcca tccatttgtg
    54421 gacaggcgga aactcaaaaa ggttagacca gtggtgtctg aggttggagc tttgagcctg
    54481 accagcaggc ttgaggagac catgagttca tcttgttatg gattgtgtta tcacaattcc
    54541 aataagaaga actttaagtc cagcctccag tcctgtcagc ccgaagtata ctacggtcgc
    54601 tatgatgtgg ttctccatac ttcgggtcta actggaagta ctgaacactt tcgcgtcagg
    54661 ttctccgaag ttcattcctg tttgactaaa ctatcactat agggaccata tctcgcctct
    54721 gacctcctga gagacggcaa gcgagaaacc gtggtccacg ctagcttgct agccaccagc
    54781 ctctggtcag cctttgtgtt ctttggtctc gtgacttaag tctatcacca cacacgctcg
    54841 gacatgctgt cgaaccttca tctctatggt catcgcagac cactaacccc aattacctca
    54901 agtcattctg tttgcgtcct gttgcctgct ggtctccctt tacgtggggg cttgagtagg
    54961 tcagtggggg tgcgacttca gggtgcaaac aaaaggagta cacgtaaatg tctcaacaag
    55021 aaatcagcgt cattaacctc gaccagcttg tctccatgac ctcagttgaa attgctgagc
    55081 tgacaggcaa ggagcaccga aatgtcctac gtgatattcg caatatggct gaggagctta
    55141 acgcgctcaa aactgagcag tgctcaaaac tgagcagtca tatcggggtg actgaagatg
    55201 tctatctaaa cgcgcagggg aaacagcagc ctctgtaccg tctcgaccgt aagcatactt
    55261 tcattctggt cgctggctat tccgttcacc tccgcgccaa gtgttacgac catattcaga
    55321 cgttagagcg tcgagtcctt caactggaag accagaagaa gcgagcagcc attcagtccg
    55381 ctaaccgccg aggcgtgact tggggcgact actgcaaaac ctatggtcta cccgctcaga
    55441 agctaatgac cgcacttctt cagcatcgtg gtctctttcg taagaaccca atcagtaacg
    55501 agtggtctgt aaatccaaag tattccgact gcttccgtat cattaagccg tcagaccaga
    55561 agttctcagc aggtggttat aactttcgct tcaacgctaa gggattggag gtcttcggta
    55621 agcctgcgat agtcgataaa ctgcgaggca tccttattgc cttcactggc accgaccagc
    55681 agaagcaaga gcatctcctg aagcaagccc aatccggcaa gctggaggga ctctaagatg
    55741 attgacccgc gcacacctat tggtcgtgca actctccgct accgtggtct gcctacccgt
    55801 cacctcctgt ccctgctgag acttggagtc gataaccctg atcgccctta ctacagtcga
    55861 gacgagctga tagcgatgct ggtcgaccgt gatttgaata atcagttacg tcgagccttt
    55921 gcgaaactgg agtcttaaca tctccggaaa tttccagacc ttaaccaacc ttatttaaaa
    55981 ccttttgaga tgaccgatgc ggggagaccc gtaggcatta ctgtgtctgc gataccgata
    56041 gtctcaactc tcaagaggag taattactat gtcta
//
LOCUS       DAZGOB010000018        52544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGOB010000018 DAZGOB010000000
VERSION     DAZGOB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.018"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..752)
                     /locus_tag="ACHLIF_001114"
     CDS             complement(<1..752)
                     /locus_tag="ACHLIF_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO9605168.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYL"
     gene            760..1261
                     /gene="tnpA"
                     /locus_tag="ACHLIF_001115"
                     /pseudo
     CDS             760..1261
                     /gene="tnpA"
                     /locus_tag="ACHLIF_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1279..1947)
                     /locus_tag="ACHLIF_001116"
     CDS             complement(1279..1947)
                     /locus_tag="ACHLIF_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO9605169.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(2250..2843)
                     /gene="tehB"
                     /locus_tag="ACHLIF_001117"
     CDS             complement(2250..2843)
                     /gene="tehB"
                     /locus_tag="ACHLIF_001117"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO9605170.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2840..3832)
                     /gene="tehA"
                     /locus_tag="ACHLIF_001118"
     CDS             complement(2840..3832)
                     /gene="tehA"
                     /locus_tag="ACHLIF_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO9605171.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3956..4936
                     /gene="ydcK"
                     /locus_tag="ACHLIF_001119"
     CDS             3956..4936
                     /gene="ydcK"
                     /locus_tag="ACHLIF_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO9605172.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDGVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4931..5467)
                     /gene="rimL"
                     /locus_tag="ACHLIF_001120"
     CDS             complement(4931..5467)
                     /gene="rimL"
                     /locus_tag="ACHLIF_001120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO9605173.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(5530..5754)
                     /locus_tag="ACHLIF_001121"
     CDS             complement(5530..5754)
                     /locus_tag="ACHLIF_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO9605174.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5894..7549)
                     /gene="opgD"
                     /locus_tag="ACHLIF_001122"
                     /gene_synonym="mdoD"
     CDS             complement(5894..7549)
                     /gene="opgD"
                     /locus_tag="ACHLIF_001122"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO9605175.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7774..9117)
                     /locus_tag="ACHLIF_001123"
     CDS             complement(7774..9117)
                     /locus_tag="ACHLIF_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9605176.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            9334..10257
                     /locus_tag="ACHLIF_001124"
     CDS             9334..10257
                     /locus_tag="ACHLIF_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9605177.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(10295..11935)
                     /gene="trg"
                     /locus_tag="ACHLIF_001125"
     CDS             complement(10295..11935)
                     /gene="trg"
                     /locus_tag="ACHLIF_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO9605178.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            12334..12516
                     /locus_tag="ACHLIF_001126"
     CDS             12334..12516
                     /locus_tag="ACHLIF_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO9605179.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSRLGQARGFSPRI"
     gene            complement(12555..12728)
                     /locus_tag="ACHLIF_001127"
     CDS             complement(12555..12728)
                     /locus_tag="ACHLIF_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605180.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12973..13503)
                     /gene="cybB"
                     /locus_tag="ACHLIF_001128"
     CDS             complement(12973..13503)
                     /gene="cybB"
                     /locus_tag="ACHLIF_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO9605181.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            13692..14693
                     /gene="gap"
                     /locus_tag="ACHLIF_001129"
     CDS             13692..14693
                     /gene="gap"
                     /locus_tag="ACHLIF_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO9605182.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFLWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(14735..16174)
                     /gene="aldA"
                     /locus_tag="ACHLIF_001130"
     CDS             complement(14735..16174)
                     /gene="aldA"
                     /locus_tag="ACHLIF_001130"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO9605183.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPAAEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(16371..17171)
                     /gene="ydcF"
                     /locus_tag="ACHLIF_001131"
     CDS             complement(16371..17171)
                     /gene="ydcF"
                     /locus_tag="ACHLIF_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO9605184.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(17443..21345)
                     /gene="hrpA"
                     /locus_tag="ACHLIF_001132"
     CDS             complement(17443..21345)
                     /gene="hrpA"
                     /locus_tag="ACHLIF_001132"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO9605185.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRIDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            21546..22151
                     /gene="azoR"
                     /locus_tag="ACHLIF_001133"
     CDS             21546..22151
                     /gene="azoR"
                     /locus_tag="ACHLIF_001133"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO9605186.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAVPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(22202..23518)
                     /locus_tag="ACHLIF_001134"
     CDS             complement(22202..23518)
                     /locus_tag="ACHLIF_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO9605187.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(23508..25265)
                     /locus_tag="ACHLIF_001135"
     CDS             complement(23508..25265)
                     /locus_tag="ACHLIF_001135"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO9605188.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(25281..26177)
                     /locus_tag="ACHLIF_001136"
     CDS             complement(25281..26177)
                     /locus_tag="ACHLIF_001136"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO9605189.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(26177..26782)
                     /locus_tag="ACHLIF_001137"
     CDS             complement(26177..26782)
                     /locus_tag="ACHLIF_001137"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO9605190.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(26953..29259)
                     /locus_tag="ACHLIF_001138"
     CDS             complement(26953..29259)
                     /locus_tag="ACHLIF_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO9605191.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNNTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(29323..30183)
                     /gene="pdxI"
                     /locus_tag="ACHLIF_001139"
     CDS             complement(29323..30183)
                     /gene="pdxI"
                     /locus_tag="ACHLIF_001139"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO9605192.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(30414..31004)
                     /gene="paaY"
                     /locus_tag="ACHLIF_001140"
     CDS             complement(30414..31004)
                     /gene="paaY"
                     /locus_tag="ACHLIF_001140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO9605193.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(30986..31936)
                     /gene="paaX"
                     /locus_tag="ACHLIF_001141"
     CDS             complement(30986..31936)
                     /gene="paaX"
                     /locus_tag="ACHLIF_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO9605194.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(32037..33350)
                     /gene="paaK"
                     /locus_tag="ACHLIF_001142"
     CDS             complement(32037..33350)
                     /gene="paaK"
                     /locus_tag="ACHLIF_001142"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO9605195.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGGAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(33377..34582)
                     /gene="paaJ"
                     /locus_tag="ACHLIF_001143"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(33377..34582)
                     /gene="paaJ"
                     /locus_tag="ACHLIF_001143"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO9605196.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPHSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(34582..35004)
                     /gene="paaI"
                     /locus_tag="ACHLIF_001144"
     CDS             complement(34582..35004)
                     /gene="paaI"
                     /locus_tag="ACHLIF_001144"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO9605197.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(34994..36421)
                     /gene="paaH"
                     /locus_tag="ACHLIF_001145"
     CDS             complement(34994..36421)
                     /gene="paaH"
                     /locus_tag="ACHLIF_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO9605198.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(36423..37211)
                     /gene="paaG"
                     /locus_tag="ACHLIF_001146"
     CDS             complement(36423..37211)
                     /gene="paaG"
                     /locus_tag="ACHLIF_001146"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO9605199.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(37211..37978)
                     /gene="paaF"
                     /locus_tag="ACHLIF_001147"
     CDS             complement(37211..37978)
                     /gene="paaF"
                     /locus_tag="ACHLIF_001147"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO9605200.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(37975..39045)
                     /gene="paaE"
                     /locus_tag="ACHLIF_001148"
     CDS             complement(37975..39045)
                     /gene="paaE"
                     /locus_tag="ACHLIF_001148"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO9605201.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGERLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(39053..39550)
                     /gene="paaD"
                     /locus_tag="ACHLIF_001149"
     CDS             complement(39053..39550)
                     /gene="paaD"
                     /locus_tag="ACHLIF_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO9605202.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(39565..40311)
                     /gene="paaC"
                     /locus_tag="ACHLIF_001150"
     CDS             complement(39565..40311)
                     /gene="paaC"
                     /locus_tag="ACHLIF_001150"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO9605203.1"
                     /translation="MNQLTAYTLRLGDNYLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSCGWLERLGNGIDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(40320..40607)
                     /gene="paaB"
                     /locus_tag="ACHLIF_001151"
     CDS             complement(40320..40607)
                     /gene="paaB"
                     /locus_tag="ACHLIF_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO9605204.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(40619..41548)
                     /gene="paaA"
                     /locus_tag="ACHLIF_001152"
     CDS             complement(40619..41548)
                     /gene="paaA"
                     /locus_tag="ACHLIF_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO9605205.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            41833..43878
                     /gene="paaZ"
                     /locus_tag="ACHLIF_001153"
     CDS             41833..43878
                     /gene="paaZ"
                     /locus_tag="ACHLIF_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO9605206.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            44126..46399
                     /gene="tynA"
                     /locus_tag="ACHLIF_001154"
     CDS             44126..46399
                     /gene="tynA"
                     /locus_tag="ACHLIF_001154"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO9605207.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(46457..47956)
                     /gene="feaB"
                     /locus_tag="ACHLIF_001155"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(46457..47956)
                     /gene="feaB"
                     /locus_tag="ACHLIF_001155"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO9605208.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            48192..49097
                     /gene="feaR"
                     /locus_tag="ACHLIF_001156"
     CDS             48192..49097
                     /gene="feaR"
                     /locus_tag="ACHLIF_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO9605209.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(49269..49598)
                     /locus_tag="ACHLIF_001157"
     CDS             complement(49269..49598)
                     /locus_tag="ACHLIF_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO9605210.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(49603..49788)
                     /locus_tag="ACHLIF_001158"
     CDS             complement(49603..49788)
                     /locus_tag="ACHLIF_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO9605211.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(49785..52424)
                     /locus_tag="ACHLIF_001159"
     CDS             complement(49785..52424)
                     /locus_tag="ACHLIF_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO9605212.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNSEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT        13267 a        13835 c        12411 g        13031 t
ORIGIN      
        1 tgaaggtagt ccctgcggat atttgcgata caggaatgca gtcgctgtat tttgcgtttc
       61 tgcttctgcc agttgttgct gaatttgacc ttgcggctta actgtcgctg aagtctcgcc
      121 agcgtcttct ggtttttctg aaaactgttt acaggctcaa agactgtgcc atctgacagc
      181 gtggcgagtt tagccacgcc agcatccagc ccgaccattg atgctgaagg gtgagccgga
      241 gtggatactt cactttctgt ctgaatactg atgtaccact taccgcagga ctggctgaca
      301 gtgacatttt tcacaacacc cgtgacctga cggctattcc ggtagcgcat ccagcccagt
      361 ttcggcagaa aaatacggct gttttcctga tcgagcttaa cgccctgcgg gtagcggaat
      421 gcatcattct gtccccgctt tttgaatcgg ggaaaagcag cccgcttccg gaagaagttt
      481 ttgtaagccc gctcaaggtc tttcagtgac tgttgcaatg gctgtgaggg agaatcttta
      541 agccattgcg tttcagtggc atttttccac tcaaccagcc aggaagccat tttcccgtaa
      601 gggatgtatt tattaccggc ctcatgattc tcattctgac gtgccagagc acgattgaaa
      661 acgaaacgac atgcgcctgc gaagcgcctc atttcacact cctgttgacc acctggtctt
      721 aactggaatt taaatgcctg taatcgtttc acaggtcaaa tgatactttg gtctgtgaat
      781 aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc
      841 tttgtcacca gataccgacg ccagattttt tgacgcgaca gaaaaactac gcacttactt
      901 ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca
      961 cgtccatttg ttaattaact aacctcccaa actggcgata tccagtctgg taaacagcct
     1021 caaaggcgta tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta
     1081 ctacaaaggc gttttgtgga gtcctggcta ttttgccagt agctgcggag gtgcgccaat
     1141 atccgtcatc cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcac
     1201 cttatatccc cgccctgaag gatggggttt tacggcacac cggataaaaa gcgccatcta
     1261 acgatggcgc gtctggaact actttttgtt aacgtcaaac atggtggcat cggtcgccat
     1321 gtcatcaata acttgtttaa tattttcgaa agccattggc gtactttcgt tattcaggtc
     1381 tttaccttcg ccctgacgaa cgactttgat aaccggttta ttagtcgctg catcaatcag
     1441 ctcaccttca aaatagaggc gagtatccat ggtacggtgg cctgtagcca tttgcgtccc
     1501 cgcaaccact aatgcaacag gaaccacttc atagaattgc agcccttctt tgctggtatc
     1561 tacaccggta atggccccac ggaaaatcag actacgcggc ccagcggtgg taaccagtgg
     1621 tttacgctgc gctatcgctt ctttcatttc ggtgttggta tagttcaaaa ttttatccag
     1681 aactttctgc cctacctggg tagaaggttt tggcaccgga taataagtga ttgggttcca
     1741 gacgatgctg tcatatttgc tttgatcaaa actcgggtct acccaacgta aaacaggttt
     1801 acctgttgct gaggttgttt cttttaaatc agagtaattg tttaaaaaac cagaatattt
     1861 atctggctgg gtgattttag aggcacaacc agacagagcc aacaagccgc ataaagctgc
     1921 aactttcgca aatgatgtgg tacgcatgaa tagtttcctg agatgattat attacttttt
     1981 aagttatagc aaaaagcgcc gcttcgtgtt gtggcaaaaa aagtgttggt gaaaataaat
     2041 ttttcagccc cttttcagac gacatagtca atgccgaaaa aggggctaag tcaatcatca
     2101 gtagttaaat ttcagtaagt taagctgcta aaaaacttcg actactgtga ttaatttcac
     2161 atcccttcga taaaaaatat gcttaccgaa aagaagaaca gtaaatcatc tcagcgtggg
     2221 gaattttcac gcgccttatt cttagcgggt cattttttac gtgccagcat cgtggcgaaa
     2281 cgcagtttaa tacgattacc gttggcgtcg gtgcggtgca gctcgccgac gtcttcattg
     2341 tatttcaccc tctcccagcc ttcgtaatat cgacgtaatt ctccctcttt gaaggcaaac
     2401 gggaagccga cggtacatgg ataatcagcg gtatccatcg ccgccacaat caggttgtaa
     2461 ccaccaggtt tagtgcaacg ttgcatattg gcaatcagcc ctgggatggt tttagcctca
     2521 aggaacatca gtaccacagt cgaaagaata aaatcgtact gtctatcaaa tgtgaggtta
     2581 ttcagatcaa cgactcgggt gtgtaaatta tccagatttt caatggattt aatgcgctct
     2641 acattggcga tactcatggc atttttatcc catgcgtcaa catcataacc attggctgcc
     2701 aggtaaagac tgttacgacc attgccacag cccagatcca gcgttttacc cggtttaacc
     2761 actttcaccg cttccagtac ttcagagtgt gtgcgggtta attcatattt atcagtaaaa
     2821 tagttttcgt cacgaatgat cattctttgt cctctgcttt cattaaaacg gcgcgctcgg
     2881 ttctgactaa caattttccc tgcatcagaa gcgcaaaagt acggatgagc agtattgcaa
     2941 taataaaatt ggtaaagata aacagcggta ccgccagcgt atggaaaaat ccattttcgc
     3001 tgccactccc cagatgcaaa ccggtggttg ccagtgcaga tacgccgaac gagaaactcc
     3061 agaatgaagc attaaatggc tgggagagat accatggcat cagacgtagc ataaacagca
     3121 gttgcagcag tccataacca aaaagcattt tcgccagcgt atcaccctcg ccgccgttga
     3181 cgctcaacca ggcactacag gccaccagcg caggagcgag ctgaatgccg agtgatgtcc
     3241 gcagtgccgt gggtaattct cccgaactgc gcagacgctg caagatcaca ggttcaaggc
     3301 ttagccatga gaaaacgcct gcgcctaaaa acaccagacc ggcgtcggtg tagcccaacg
     3361 caccacaggc cattgcgctg ataaagttgt tggcaactgt cggcagatac agtccaggcg
     3421 tggtagcttc ttcagggtga gatccgcgcc ataatcccgc agtttgccag gcggcataag
     3481 ccaactgaac cacgacacca aaactgaaca ggcacaccgc cagtgggcga aaccacggaa
     3541 caaaaccaat cgccaccagc atcgtcgttg ccggaaacaa actcacaaaa ctgctcagca
     3601 ctggatggcg aacttccgcc agcacgctat gcggaaagcg tatgagtcgg gtaataaatg
     3661 cgctagttaa taatccccag atgatcatcg ccagaatcac cagcccatcc cctaaccagt
     3721 ggctgacctg ccaaacctgg ctggcatagc gccaggcaaa tcccatccct atcgtcccca
     3781 acacaatacc aaagtagcct gccggcaaat tgagcacttt atcgctctgc atcttcttac
     3841 tcattttgtt taatttataa agtatatttg aaatgcattt tatggaattt tctcgagcat
     3901 agccagagcc gcagaatttg ctacggttat agtaagtcac ctgtgggaaa acgctatgcg
     3961 caaatatcgt ctaagtgaag aacagcgagc ctttagttat caggaagatg gcactaaaaa
     4021 aaatgtgtta ttacggcaga ttatcgccat tagcgatttt aacgatgtga tagcaggaac
     4081 cgctggcggc tggatcgatc gcgaaacggt gttagcacag gaaggaaatt gctggattta
     4141 cgaccagaat gctattgcgt tcggcggaac ggtgatttcc ggtaatacgc gcatcaccgg
     4201 caccagcgta ctgtggggag aggtttacgc aacggataat gtctggatcg ataatagtga
     4261 aatcagccag ggtgcatata taagtgacgg tgtcaccatt catgactcac tggtttgcgg
     4321 gcaatgtaga atatttggtc acgccttaat taatcaacat tctatgatcg tcgcagcaca
     4381 aggcttaacg cctgaccatc aactcctgct acaaatttat gatcgcgcaa gggttagcgc
     4441 ctcgcgtatt gtccaccagg cacaaattta tggcgatgcc gtcatccggt atgcttttat
     4501 tgaacatcgt gccgaagttt ttgattttgc cagtattgaa ggtaatgaag agaacaacgt
     4561 ctggctatgc gattgcgcta aagtctacgg tcacgcgcag gtgaaagctg gcatagaaga
     4621 agatgccatt ccaacgattc attacagttc gcaggtggcg gaatatgcca ttgtggaagg
     4681 taattgtgtg ttaaaacatc atgtcttgat tggcggaaat gccgtggtac gtggtggacc
     4741 gattctgctc gacgagcacg ttgtaataca aggtgagagc cgtattacag gtgcggtgat
     4801 aattgaaaac catgtggagt taaccgacca cgccgtcgtt gaagcctttg atggcgacac
     4861 ggttcatgtc cgtggtccaa aagtcattaa cggcgaggag cgcattacgc gaacgccgct
     4921 ggcagggtta ttatgaatcg ataatacgcg cgtataagtt cacatcatca taggcatcat
     4981 tcaggaactc agcctgtttc aggcaacctt caaggataaa accattgcgc aaagcgacct
     5041 ggttgctttg cggattgtcc acccgacatt tgatcacgaa gcgtctaagt tcaccagact
     5101 gggcgtaatg atgaatcaat gcctgcagcg cctgagaaat gatcccctgc ccctgatgag
     5161 attcgtccag ccagtagcct atttcagcgg ttttattcag tggttcaata cgattaaacg
     5221 agataacgcc gataagttca tcttctttga aaatcatgaa cattttggca tagccgcgtt
     5281 gatgcaacat cacattaccc tgcaccgttt ttcgcgtgtc ctcttcactc tgaacgaact
     5341 gcggccagtt tagcgactgc tgtaaccagg ttttattttt acagattaac tgataaagag
     5401 gagtgacgtg attttctgca acagcatgta attcaagtga ttcgcttact tttatcgttt
     5461 cagtcatctt atctccagtc aatccatgca gttgttaaaa aagccgcctg gctggcggct
     5521 taggaaagtt tacacctctt tgacgctctc ctgctgcagg attttgagca tctcatcttt
     5581 taactgtagc ttttgttttt tcatgcggac cacttccgca ttgtaccctc gaccgtcgga
     5641 accttccttt ctggcaattt catgatcaag tttattgtgt ttatcgaaca aggacataaa
     5701 gcgaggattt tcgtttttca gacgggatat taaatctcga tattctggaa acatacgcac
     5761 tccctgttag tggctgacaa ggtgtatctg aaatacacac catcagatta ctcatttaac
     5821 ttagggaaaa atgcgagcgg gatcgtgttt aaccagacgg cacctcccga agaaggtgcc
     5881 gaagaaaaaa cgattaactc atcacgcggt cgtcaacata ctgacgttta tccggcgctg
     5941 gcgggaaata ctgatacagc catgtttcac tgatagcgtc gccctgacaa cgcagataca
     6001 tccgcatatc gaccggatca gtggagtccg aagtcggata ccagtcaaac tgaatacgat
     6061 aaccatcaat gggttcaata tagagaattt cgatttgctt cgcttcccca ctggaaagcg
     6121 taatcaccgg ctcaatgcct tttggtgcgg cagctttcag atcaccacca acgaaatcga
     6181 cggcaaaacg acgcgcccat ttttcgggat agtgttcacc tggtgcccaa ccttccggga
     6241 aaccgcccat gccggtacgc gtcgccataa cgcgcgctaa tgggcaatga acaggcggtt
     6301 gcgcactcca gtacagacga tactggaatg caaactcatc acccgccttt acagcttttt
     6361 ctggctgcca gaagcagaca atgttatcca gcgtttcgcc cgttgttggg atttccatca
     6421 ggccgatggt gcccttaccc cacttgttac gcggttccac ccacagactt gggcgtttgt
     6481 tataccagcc cataatgtcc tgataatggg agaaatcacg atccagttgc aataaaccaa
     6541 accctttcgg gttgttgtcg gtgtaagcat tgaactgcaa tttttgcgga ttattcagcg
     6601 gacggcaaat ccactcgccg ttgccccgcc acatggacag acgatcagag tcatgaattt
     6661 gcggatgaat ggtgtcgcac atccgacgtt cattagtacc gcagctgaac atactggtca
     6721 tcggcgcaat gcccagctgt ttaatgtctt tgcgcgcata caggtgattt tccacatcca
     6781 taatcacctg acttttctca caatggatag tgaacttata ggcaccagta atgctggcgc
     6841 tatcgagcaa cgcataaacg gtaaaggtag ttgcccccgg ttttaccgta tcaaaccaga
     6901 aggcggtaaa gtcggggaac tcttctttac tgtcggtgta agtgtcaatc gccaggccgc
     6961 gggccgacaa accgtattga tatgtgtcat caacggcgcg gaaataactc gctccgagga
     7021 atgatactac atcacggcgc gccagttcgg gggctttaaa cacgcgaaaa ccggcaaagc
     7081 cgagatcgct ttgcccttct aattgtttgg tatcaacacc tgcatcgttg tatttgaaca
     7141 actccgggcg aaagtgaatt tcacgcgcca gatgtgttgc tggatctaca gaaaacatac
     7201 gaacgcggcg acggaatccc attcccatat ggaagaactg agcgtccagt tgacggttct
     7261 caacgttatg ccagagcgat ttttcggcgt cgtattgaat actgttataa gcctgcggcg
     7321 tcattgtcgc cagcgtgtca ggtaacggac gaggcgcacc acgccacgct gtttgcgcta
     7381 agtcgtgcgc cattgactgt agaatggaga agtcaaaacg ctgggtttgc ccgtcggcaa
     7441 tatcagaatc tgccgcgaat gccgcctgag aaaaaagaga agcaatgccg ctggtaccgc
     7501 acacggcggc catagccatt gaacctttaa taaatcgtct acgatccata cctgaaagtg
     7561 agtccttctg gtgttgtgaa tggtcccgcg ctggtcgttt tcagcggcaa gaaagtgctt
     7621 atttatgacc gctcactcta gacaatattt attaataatc caattgttga actcagagaa
     7681 tatgcataaa tcggcaattt ttttatgtcg atgggatgag cctgattcgt ttgtaaaatc
     7741 aggcaaattt ttgcaaaggc ggatggtcag aatttaaagc aaaccacagc gacgtttact
     7801 gcgttcttcc gcttcctgat aaagctggaa ctcatccaaa accggacaac ccaacgcctg
     7861 ctcaaatgcc tcgcgactgg cattaccgtg attgcgtgcc tgcgtttcat tacccagatt
     7921 tgactggaag atccccgccg cgctaacggg caagaaatct tcataggtaa tgggttgcgc
     7981 cgcgacccaa ccacgttcaa ttaagggctg tggatcgtct ccgggatgaa tcgcctgacg
     8041 atgcgcctca cctgaaggcg tcagacggta ccggaaccat gccagccctt gctgacgcat
     8101 taaaaactca ctgtcaggaa aagtgcggaa ggtttcctgt aaatgcattt ggtgagtgag
     8161 attatcctgc ccggttccag cgttacgcag aagatcatca tacagttgtc gccctttcgg
     8221 cgttaatgcc acgccacgct gctcaatttc accaaagcgc gcggtatgcg tgccctgttt
     8281 ctgccccgca aacaacaccg tctcttccag tgctttaaag ctggtctggc gtagtaaaat
     8341 cggtacctcg cggcgcggcg gcccctcgat gagaattttg ggttcaattc cgcattcagg
     8401 catcatcgac tgcacccggt caatatccag cgtacgtggc gtcaggtggt tgatatggca
     8461 tccaggaaaa cagaccacat cagcaatcaa ccgatgttcg ttgtgcaagg cgcgataggt
     8521 ttcttcatct accgttgctg actggtgcca gcgaaacgtt tccagggctt cctgcacaaa
     8581 ctcctgtgcc tgtgtttcgt taaaaccgcc ctgctgctca tattcctcta acagttgtcg
     8641 acaacgtggg gtgaagatat cgcgctgacg cagaatctcc gccgctttct ggcgcaaaat
     8701 ttcgttctcg ataagctcaa ggcggagtaa ggaggtaaaa acgcggaagg gattacgcgc
     8761 cagagaagca tcatcaatgg gccgaaatgc tgtcgaatgt accggcaccc ctgcctgcga
     8821 gagatcgtaa tagctcaccg ggtacatccc cataatggca aacatccgcc gaagagtagc
     8881 aagctcttgt gcagtcccaa cgcgaatcgc cccatgacgt tcaacattca gtcgcgccag
     8941 ctcgtctgca tttaccattt tttcgtgcag ttgaggattg ttttccagca cagccagatt
     9001 cacatcagct accagttcca gcagcgtgcc atattgcgga acttcttgct ggtacatggc
     9061 tgacattgcc tgcgaaaact gttcccgaat ctcatccgcc gtgatgctgt tcgccatagt
     9121 gtctgcctcc agtggtcagt aatctggagt gtaggtaacc gcattcactc tggcgggaag
     9181 aatttacaaa ctgtgatctc gccgcgaaaa catcaatatt atccattttg ctgtaacaca
     9241 attgctttaa ttgttaataa tattttgcaa tcaagttatc ataatcaaac aacttcactt
     9301 gtcagcgaca ccgcttcgtt tttaacatcg cttatggaaa aaaatagtct gtttagtcag
     9361 cgcatccgtt tgcgccacct tcatacattc gtagctgtcg cacaacaagg aactttgggg
     9421 cgcgcggctg aaacccttaa tttgagtcaa cctgcgctct ctaagacatt gaatgaactg
     9481 gagcagctga ctggcgctcg cttgtttgag cgtggtcgtc agggggcgca acttacctta
     9541 cccggcgaac aatttttaac gcatgcagtc agagttcttg acgccatcaa cactgccgga
     9601 cagtcgcttc atcgtaaaga aggtcttaat aatgatgtcg tcagggttgg tgcactacct
     9661 actgcggcac tggggatatt accttcggtt ataggtcagt ttcatcagca acaaaaagag
     9721 acgaccttgc aagttgcgac aatgagtaac cctatgattc tggcgggttt aaaaaccggg
     9781 gaaatcgata tcggcattgg tcggatgtca gatcctgaac tgatgaccgg gcttaattac
     9841 gaactgctgt ttcttgaatc gttgaagctg gttgtccgcc ctaatcaccc gctacttcag
     9901 gagaacgtaa cgctaagccg ggtgctggaa tggccggtcg ttgtatcacc agaaggcact
     9961 gcgccacgcc agcattcaga tgcattagtg cagagccagg gctgtaaaat tccttcgggt
    10021 tgtatcgaaa cgctgtctgc ttcgctatct cgtcaactta cggttgaata cgattacgtg
    10081 tggtttgtcc cttctggcgc tgtaaaagac gacctgcgtc atgccacgct ggtggccctg
    10141 cctgttccgg gacatggtgc aggcgaaccg attggaatac tgacccgcgt agatgcgacg
    10201 ttctcttctg gttgccagtt gatgattaac gctattcgaa aatcaatgcc gttctgaaag
    10261 gtgaagggat ctgtcgatcc ctccttgaac attttcacac cgtagcgaaa ctaactggtt
    10321 cacccgctcc gcgaggttct gccgacacag aatgtttgtg cagacggaat acatccaccg
    10381 cctcagttaa tcgtgccgcc tgttcttcaa gggacaccgc cgccgctgag gcctcttcta
    10441 ccagagaagc attctgttgc gtcaccttat ccatttcaga aatcgcctgg ctaacctgcg
    10501 ttatgcctct actttgttca tccgaggcgg cggcgatttc ctgcatgata tgtgtgacac
    10561 tcgcgacggc atcaacaata gtgctcatgg ttttccctgc cgttgccacc tcatccgacc
    10621 ccaggtcaat taacctgact gattcactga tcaagccttc aatctctttc gccgcttggg
    10681 cgctgcggct tgcgagtgtc cgtacttcgc tggcgacaac ggcaaatcca cggccttgct
    10741 cacccgctcg cgcggcttca acggcagcat tcagtgccag aatattcgtc tggaaagcaa
    10801 tactgttgat gacggcggtg atctcggaaa ttttcttcga acttgtagag atagcgccca
    10861 tcgtttttac tacaccggaa accgtctgcc cgccatcgct ggctttaata gaagcctctt
    10921 gcgccagttt gctggcatga tgcgcgttat ccgcattctg tttcaccgtc gcagtgagtt
    10981 gctccatgct agcggcagtt tgttcgatag ccgccgcttg ttcttcggtg cgagatgaca
    11041 ggtccgcatt gccagctgaa atttcgctgg tgccacgata aatctcttcc gcaccctgtc
    11101 gaacagtccc tactgtcatc cccagtgaat gctgcatttg ctgtaaatga cgacttaagc
    11161 gaccgatttc attacgaccc gccggttcat cattcatcgt cagatcgcca ctggcgattt
    11221 tttcaatccg ttgtgcggca tgttgcagtg gacgaatgac gatccgacgt agcaccataa
    11281 atgttatcag cgtcatgacc agggcaagca caaacgcgcc aatcatgaac atcccaccca
    11341 ggcgggtgcg ctgatgggcc agttccgcca gttggttggc tctggtgcta cgtatcttaa
    11401 cggctttgtt caaaatatcg gtataagcat tatccagcgg tcggatctgc tcactttcat
    11461 gattgataat cgcttcaaac atgccatttt tggcatattt caacataggt tgcatacccg
    11521 tgatataagc ctgaaagcgt tgatttaatt cagtgtcgag ggcttcatca gcaggtgttt
    11581 tcaccggtcg attctgataa gcacgataac cttgctgcga ctgtttaatc tccgattcgg
    11641 cttgcgcaat atttcgcttc attgcttcca tttccgcaat acgacttgca gccccggcct
    11701 gaatcatatt gatccgggct gaacgcaaaa aatctgaact gttcgctaac ccggtccgaa
    11761 tctcaatctc cgcagtaaca tcacgctgat cgcgatcggc ctgccagagg aaatagccag
    11821 ccagagcaga acttaatgcg aatagaacta agataccgcc aagaatgcag gcaaataacg
    11881 gaaccagcct gatgtgatgc aaaaaaccta atcgatgtga gggagttgta ttcattaccg
    11941 ttgtctctcg tccaggttta ctcctgatag aaagtcatcg gcacttgacg gtaattactt
    12001 atgaccaaaa acctctttgc gtcacatttt tacaacaatt attgcagaga aaattaatgc
    12061 tattggaatg aagagagtaa cgaatggcta aactggctaa cccgaatgac gaaaaattct
    12121 ggaaaacagc acgcaatttc gtgaagtgat tattttcgtc cctaaggaaa cctcatagga
    12181 tgcctgcggg tgcctgagac tttctgtttt caggctaacc gggaatcaga aagacgaagg
    12241 ccccgaccga tatttctatc aaccgaggcc aacgaatgaa cctagcaatt cagatcttag
    12301 cctcttaccc gccatccggc aaggagaaag gctatgaagc acaaccctct ggtggtgtgt
    12361 ctgctcatta tctgcattac gattctgaca ttcacactcc tgacccgaca aacgctctac
    12421 gaactgcggt tccgggacgg tgataaggag gttgctgcgc tcatggcctg cacgtccagg
    12481 ttagggcaag cgcggggatt ttccccgcgc atctgactag cagattcatt ttctcatggc
    12541 acccctttta cacttcagtt agtgcatgtt tttttcgagg cactaataga accatcatta
    12601 cagacaaatt tgccgcctgc cgtacagtgt gagatccccc ctttcgaacc agaacagggt
    12661 ttgcgcccgg catcggcata aaaggaaaca agcgttccca taaacaaaat aagtgccagt
    12721 tttttcatat aacctccatg gtttatcgaa tcttatgttc gatttgaacg ttatacgctg
    12781 gtttattttg ataccgttga tgagatcaaa gatcgggtga attgattaaa aataagccag
    12841 atggacgtat gggcaaggat tatggtttta tttgtcatac aaataaatat aataggcgct
    12901 tccgatgtag acccgtattc ttcgcctgta ccacgggtcg gttttagtac aggcgttttc
    12961 tttaaatatc cttcaggaac gtttacgcgg catcatgcgt agaagtgtgt tgtccttcca
    13021 gaaatagtgg tgtgccagtg cagccgcagc gtgcaacccg atgacaaaat atcccagatt
    13081 cgccagcgtc tcatgccacg actttaagct atcaacccgt tcgaaattgg cctctgaagc
    13141 gtaaggcatc gtcaaaccaa acgcaaacca cgggttgccc cggttataca tcatcaccaa
    13201 accaatcact ggcagcgcaa taaacaggag ataaatcacc aaatgtccca aatgcgccag
    13261 tcccgtcatc atcggttttg gcttaggtat aatcggcggg gttgggtatt tcagtcttaa
    13321 caaaagacga accaccatca gcacgagaat tgagatacca caggaaacat gaaccatgtt
    13381 gatgagtggc cgatcgctac gtgggaagaa accacgaaac tccattgcgc aatacgctgc
    13441 gataaccagc aaaaagacca gccagtgaat gctgatttgt aaccttgagt atttattttc
    13501 cataacattt cctgctttaa cataattttc cgttaacata acgggctttt ctcaaaattt
    13561 cattaaatat tgttcacccg ttttcagtac gttacgtttc actgtaagaa tggagattgc
    13621 ccatcccaca atcctggtct aagcctggaa aggatcaatt ttcatccgaa tgttcctgac
    13681 aggagaaaac catgagtaaa gttggtatta acggttttgg tcgtatcggt cgactggtgt
    13741 tgcgtcgatt acttgaagtc aaaagcaaca tagacgttgt cgctattaat gatctcactt
    13801 ccccaaaaat tctcgcctac ctgctgaaac atgattcaaa ctacggacca ttcctctgga
    13861 gcgttgattt tacggaagat tcacttatcg ttgatgggaa aagtatcgcg gtttacgccg
    13921 aaaaagaggc taaaaatatt ccgtggaaag cgaaaggtgc agaaatcatt gtcgaatgta
    13981 ctggctttta tacctccgcc gagaaatcgc aggcgcatct tgatgctggt gcgaagaagg
    14041 tgttgatttc cgcccctgcc ggtgaaatga aaactatcgt ttataacgtc aatgacgaca
    14101 ctctggatgg caacgacacc attgtttccg tggcgtcatg caccactaac tgtcttgcgc
    14161 cgatggccaa agccttgcat gacagtttcg ggatagaagt cggcacgatg acgaccattc
    14221 atgcctatac tggcacccag tcactggtgg atggcccacg tggtaaagat ttacgtgctt
    14281 cacgcgcagc ggcagaaaat atcattcccc acactacggg ggcggcaaaa gccattggtc
    14341 tggtgatccc ggaactgagc ggcaaactga aaggtcatgc gcaacgcgtg ccggtgaaaa
    14401 caggttcggt cactgagctg gtgtccattc tcggaaaaaa agtgactgcc gaagaggtga
    14461 ataacgcact taaacaagca accaccaata acgagtcatt tggttatacc gatgaagaaa
    14521 tagtctcttc cgatatcatt ggcagccatt tcggttcggt gtttgatgcc acgcaaacgg
    14581 aaattaccgc cgtgggcgat ttacaactgg tgaaaacggt cgcctggtac gataacgaat
    14641 atggcttcgt cacgcagctt attcgcaccc tcgaaaaatt cgctaaactc tgacgcgcac
    14701 aggcggagga aaaaacctcc gcctctttta ctcattacga ctgtaaataa accacctggg
    14761 tctgcagata ttcatgcagg ccatgtttac catctgcgcc gccaataccg gatttacgcc
    14821 atccggcgtg gaagccttgc atagcttcga agttttcacg gttgatgtaa gtttcaccaa
    14881 acttcagccc tttaatggct ttcatcgcga cgttcagatt ttgggtatag attgatgagg
    14941 tcaggccgta atcactgtca ttagccattg agatagcatc ttccagcgtg tcaaatgcga
    15001 ccaccggcag aaccgggcca aaggtttcct catgcataat cgacatttcc tggcgaacat
    15061 ccagcagcaa tgtcggcgga taataatatc ctttcccgtc tactgctttg ccacccaacg
    15121 ccactctcgc cccttcttct actgcgcgcg ccactttttg ctcgaccctt tccagcgccg
    15181 cggcgttaat caacggcccc atcgcaatgt cgttgcgttc agcggggtta ccaaattgaa
    15241 ccgcctgcat cgcttcaccc agccgattga cgaactgatc ataaatgcct ttctgtacat
    15301 aaacacgttc tgcacagtta cacacttgcc cactattaat gacgcgtgaa tcaacgatgg
    15361 ctttgactgc cagttcaaga tcggcatcgt ccattacgat agctggtgct ttaccaccca
    15421 attccaggca cactttggtg atgtttttcg ccgcagtcgc cataatcttc tcacctgcag
    15481 agacgctgcc tgtcatactg accattgcga cctttgggtt acccgccagt tcttgcccaa
    15541 cggtttcacc acgccccagt acaaggttaa acacgccgcg cggaaggcct atttcatcga
    15601 cgattttggc gaatgcaatc gcattgtttg gcgtaaattc actgggttta atgacgatgg
    15661 tattaccggt caaaagagcg ggagccattt tgcgggcaat gaggaagaac gggaagttcc
    15721 acggcagaat gccggtagtc acaccaagcg cacgtttaaa caaaagaata ttttctcctg
    15781 gacgatcgct ttgaataatc tcgccctcgt aacgccgtgc ccactccgcc atgtaatcga
    15841 tatagtcggc agtaaaagcc acttcgactt cagccagctg ctggatcttg cccccttctt
    15901 caacaatcag cgcactgatt tcactggcgc gttcgcggat gccggcggag attttgcgca
    15961 accaactggc gcgttcaata gcaggcaacg cttcccattc tggttgtgca cgttctgctg
    16021 catcgattgc cttacgggca tcctcggcct gaccatcggg tatgcgggaa atgacagcct
    16081 ctgcagcagg gtttaccaca tcaatccatg cgtctccacg ccaggtaaca aactgtccat
    16141 cgatatacat aggatgttga acgggtactg acatgggcga ctcctgtgat ttatatgttt
    16201 gttttcggtg aatgcattgt taattaacat caatcatcag agagacggca tcgtgaaagg
    16261 acaattctgt gaagggcttc ataaaaacaa gtgtaaaagg aataaaaggc agaaataaaa
    16321 gtcaggagtc atgcccgttt gcaaacaagt ggttcacaaa cgggcaattt ttaacgtaac
    16381 gagcgacttt ccatcgcctc gatgagcacc gcatcatgtt tcagcgtttg ccatgcatgg
    16441 atgacttctg ccggaaaatc aacgtgaacg ataaaatctc gcccgcgggg accgtagcca
    16501 tcgctatcat cgcgtaaacg cggcagctcg ccagtgagta gtgagagata acgctcaacc
    16561 ggccataatc cttgtaactg attaataaag attacactgt ctgcgttatt tcctaactga
    16621 ggaacgaatc cgggataact taaccagcgt ggtgcatcgg gattgtcccc agtcatacgg
    16681 cggaacgtcg ccatcgtgcg ccgctgcatg gtggggtcct gaacaacgat agccgtatga
    16741 actcgttcta cggcctgatt caatagcgcg atgctaaagc gtgcgttttc accgcagttt
    16801 gttgactggt cttcaatcca gattttttca tgcggaatgt gccagaactg atgagcgata
    16861 tccgccagga tggtcgcttc tgctctgcca gtggtgcgga tagtgttgta gtgcggatgc
    16921 tgtgcgatgg cgctatacaa aaaagttgtc gagtgaccga taccaccact aatcagtaaa
    16981 ggaatttgct gatcgcgagc aatcttacat gccgcgtcga tagtcggcat aaccgcattg
    17041 cctgcaagga tcacgcaatc ggcctgatac ggcacctcac cggagaaatc atcctgcgcc
    17101 agccactgtc cgataacatt tatggcatct atagttgccg gcgaaagcgt cggaaacggg
    17161 gtaatgttca tggttcctcc ttatcgttcc cttaagaata ctgcgtgaga taatagtgag
    17221 aagcggagca ctctgaaagg aggaagtcgt atgtccttaa ccgccactcc gcaagcctga
    17281 catatctttt aaggaaatgc tgtagataaa cattattcat cttgcagcag agaattgtat
    17341 ttttctgaat tcctgaattt ttacccttac cgttttagaa ttaataaaaa acccggcgct
    17401 aaatgcttac cgggtttttt ctttatcagg caaatagcag ggttaaccgc taatctgctc
    17461 catcgcctgc aaaatacgct tatctgaaat cggataaggc gtaccaagtt gttgagcgaa
    17521 gtaactaacg cgcaactctt ctatcatcca acggatctct ttcacgtctt catcctcacg
    17581 acgtgcgggc ggcagtttgt tgatccattg ctgccacgcc tgctggacgt tttcgacttt
    17641 cagcatctga gcacggtcgc gatgtggatc aaccgccagt ttttccagcc gtttttcaat
    17701 cgcctgcaaa tatcgcagcg tgtcgcccag ccgtttgaag ccgttaccag tgacaaaacc
    17761 gcgatatacc aacccgccca tctgcgcttt aatgtcagaa agccccagtg ccatggtcat
    17821 atccacccgc cctttcagac gtttgttgat attgaacacc gcagtgagga tttgctcgac
    17881 ctgcttcgca atatccacca ccgtgtcatt cagttcggcg cgcacttttt catgcagcgc
    17941 agcaaagcct tcttccgtcc agaccgggcc accattggcg tcgatcaatt gatccacacc
    18001 gcaggagata cagtcgtcga tcagctccag cactttgcca tacgggttaa agtacagtcc
    18061 cagcttggct ttgttcggta acttttcatg taaatatttg attggcgatg gaatattcag
    18121 cagcagtaga cggcgaagac cgttccacat tgcctgcttt tgctccagcg ggttatcaaa
    18181 cagtttgatc gccacactgt cgcgctcatc caccagcgcc ggccacgcct tcactttgta
    18241 gttgccacgc ttctgttcgt agctttccgg cagctgacca aaactccaga tatgtaagcc
    18301 gctctgctcg ataccgtcat ccgccaccgc agatagcgtt tcctgcactt tgcctttcag
    18361 cgcatctttc agatcttgta gcgagcgccc ttcttttagc ttcttgtttt tgtcatccac
    18421 cacgcggaag gtaattttca ggtgatcagg cacctgatcc cagtgccagt cttcgcggtc
    18481 aacggtaacg ccggtcatcc gccgtaactc gcgctcaagg ctgtcgagca acggtaactc
    18541 cagcggtttg acgcggccta aaaacgcttc ggcatagttt ggcgcgggta caaaattacg
    18601 gcgtaccggt ttcggcaacg atttaatcag agcaatcacc agttcgcggc gcagaccggg
    18661 gatctgccat tcaaacccgc tttcctcaac ctggttaagt aacggcagcg gaatatgtac
    18721 ggtcacaccg tcagcatccg cgccgggctc aaactgatag ctcaaacgca gcttgagatt
    18781 gccctgatgc cagaagttcg ggtaatccag cttgctgatt ttttctgcgc cctctttgat
    18841 caacatgctt ttttcaaagt tgagcaaatc aggggtttcg cggctgactt ttttccacca
    18901 gctgtcgaag tgacgagcgg agattacatc gtggctgatg cgctggtcgt agaactcaaa
    18961 caacgtttcg tcatcaacca gaatatcgcg gcgacgtgat ttgtgttcca gctcttccac
    19021 ttcggcccgc agtttcaggt tttcacggaa gaatgcgtga cgcgtctgcc agtcaccttc
    19081 caccagcgca tggcgaataa agagttcacg gcataacgcc ggatcgatct ggctgtagtt
    19141 gaccttgcgc gcggcaacaa tcggcaaacc ataaacagtg actttttccg ttgccatcac
    19201 cgcgccctgc gcccgttccc agtgaggttc gctgtaggtg cgtttaatca aatgctgagc
    19261 gacaggctcc acccattccg ggtcgatacg cgcagcaatg cgcccccaca ggcggctggt
    19321 ttctaccagt tccgccacca ttacccattt cggcggtttt ttgaataaac cagaaccggg
    19381 aaagatggag aaacgcgcgt tacgtgcgcc ggtatattct tgtttatcgg catctttcat
    19441 gccgatatgg gaaagtaaac ccgtcagcaa cgcaatgtga atttcgcgat actccgccgg
    19501 ttcgctgtta accggaatgc caagttcttt caccacctgg cgcaactggg tgtagatatc
    19561 ctgccattcg cgcacgcgca gatagttgag ataatcgata cgacacaggc gacggaaggc
    19621 gttggaagaa agcgcctttt gctgctcgcc aagataattc cacagattca caaacgcgag
    19681 gaagtcagac tctttgtcgt ggaagcgacg atgtttttcg tccgatgcct gctgtttatc
    19741 catcggacgc tcgcgcggat cctgaatgga aagcgcggac gtgataatca tcgcctcacg
    19801 cacgcagcca tgtttttgcg cttccagcac catacgcgcc agacgtggat cgacaggcaa
    19861 ctgcgagagc tggcgaccga gcggcgtcag tttataggcg ctggcctgtt catccgtggt
    19921 gatcgcgccc agttcttcga gcagacgcac gccatcctgg atattgcgtt tatccggtgc
    19981 ttcgacaaac gggaacgcag cgatatcgcc cagccccagc gcggtcatct gcaaaataac
    20041 cgaggccagg ttggtacgca gaatctccgg atcggtaaac tccgggcgcg aaaggaaatc
    20101 gtcttcggaa tagagacgaa tacagatccc ttcggacaca cgcccacagc ggcctttacg
    20161 ctgattggca gacgcctgcg aaatgggttc aatcggcaaa cgctgcactt tggtgcgata
    20221 gctgtagcgg ctgatacgtg ctgtaccggg gtcgataacg tatttaatcc ccggtacggt
    20281 cagcgaggtt tccgcgacgt tggtcgccag cacaatgcgc cgtccgctgt gcgactggaa
    20341 cacgcggttc tgttcgctgt tcgaaagccg cgcataaagc ggcaagattt cggtatgacg
    20401 cagatttagc ttgttcagcg catcggcggt atcgcgaatt tcccgctcac cactcataaa
    20461 gatcagaatg tcgccagggc tttcctgact cagttcgtct acggcgtcaa aaatcgcctg
    20521 caactgatcg cgctcggtgt cgtcggcttc ttcaacaatc ggtcgatagc gcacttccac
    20581 cggataggtc cgaccggaga cttcgataat cggcgcatta ttaaagtggc gcgaaaagcg
    20641 ttccgggtcg atagtcgcgg aagtgataat gatttttagg tcaggacgcc gcggcagcaa
    20701 ctctttcaaa tagccgagca gaaaatcgat attcaggctg cgttcgtgtg cttcgtcaat
    20761 aatgatggtg tcgtactgca tcagcaggcg gtcttgctgg atctccgcca gcaggatacc
    20821 gtcggtcatc agcttgacca tcgtgttatc actaacgtga tcgctgaaac gcactttgta
    20881 accgatgcaa ccgcccggct ccgttttcag ctcttccgca atacggttcg ccaccgttct
    20941 tgccgccagg cgacgcggct gggtatggcc gatcagccct ttaatcccgc gccccagctc
    21001 catacagatt ttcggtaact gagtcgtttt accagaaccc gtttccccgg cgacgatcac
    21061 cacctggtga tcacgaatcg cttcgagaat gtcctgtttt ttctgactaa ccggtaaatt
    21121 gtcaggataa gtaatttccg gtcgtgccgc ttcacgcagc aggactttcc ctgccgcctg
    21181 gtcaatctct ttcgccatct cctggaaaat ggcctgttgt gcatcaggat ttttaacctt
    21241 cttaacaccg tgcagacgcc gagaaaaacg cagtctgtcg cgcagcatca gcgaatccag
    21301 ccgctgctgc aaggccgtaa aggtcaattt ttgttgttct gtcataacgt tagtgggcag
    21361 tagtctgcct gattaatttt cttttaaatg atagatatag agtaccacat cgcggctttc
    21421 tatgcgttgt tcaataaatt cgaacatagg cttcgattta ttgcgctatc tctgtggcag
    21481 gattgtgaat aaagtgtcag caagcaacgg ggcatcgccc attcaaacat ctataaggaa
    21541 acaccatgag caaggtatta gttcttaaat ccagcatcct ggcagggtac tctcagtcta
    21601 atcagttgtc cgattatttt gttgaacaat ggcgcgaaaa gcactccgct gatgaaatca
    21661 ccgttcgcga cctggctgca aatccgattc cggtactgga tggcgaactg gttggtgcac
    21721 tgcgtccgag cgatgcgccg ctgactccgc gtcagcagga agctctggcg ctttccgatg
    21781 agctgattgc cgagctgaaa gcccacgacg ttatcgttat tgcggtaccg atgtataact
    21841 tcaacatctc aactcagttg aaaaactatt ttgacctggt tgcacgcgca ggcgttactt
    21901 tccgctatac cgagaacggt ccggaaggtc tggtaacggg taaaaaagcc atcgttatta
    21961 ccagccgcgg tgggatccac aaagatggac caacggacct ggtgacgccg tatctgtcca
    22021 cgttcctcgg ctttatcggc attaccgatg tgaaatttgt cttcgccgaa gggatcgcat
    22081 acggtccgga aatggcagcg aaagcgcagt ctgacgcgaa agcggccatc gacagcattg
    22141 ttgctgcata accggacacg atcttgatgt ggactgtagt tcagtccaca tctcaatcca
    22201 cttacctgtt ttcccataat ttcagcatcg ctttgtgatc ttccttaagc acaatatgcg
    22261 aacgtctggc tcgaatataa ctaatcgctt catcaacggt tttacagtgt ccgtaacata
    22321 acaaccatgc cgccactacc agcgcactgc gcgataatcc caacgcgcaa tggaccagaa
    22381 cgctgccttg ctcttcgcgt aatgtttcca gcatcgccac ggcctgtcgg agctccccct
    22441 cttccggaac caccagatcc agcatcggta cacagaaata gagtcgatct tttgtcgctc
    22501 gtccccgagg gaattcaaag gtgacgtcca gaaccgcatt ctgtgccgga atatgtcgtg
    22561 gaaacgcccc taaataaaca ccagcagtaa ttttgctcac cggctccagg cgacgacaaa
    22621 accaacgcat agacagccac atcccgatgc gccagggcaa tgtcagccag taaacggcgg
    22681 gcgggagttt cccctgacta tctttccctg ttgttatcgc gccaagccca ccgtacccac
    22741 ggccaataat gagtagcgat aatactggcc aacataacca gactgaccac cataactgaa
    22801 tcatcaccat tagctccatc aacacaatgc acgagcccgc gcctacgaca tatggcagtg
    22861 ctattttgat tcgacgttga tcaggtttct gataattcca acgacgatcg acgggcacca
    22921 tccagtcaat caacatacct accgccagcc ctgtgatgac atcaataaaa tgatgctgcc
    22981 aggtcgtcag agtagaaata gcgatgagta aaaaccatcc gccacagact ttacgccacc
    23041 tctcagccag atgctgacga aagtgacgcc aaagtagcca gcagagaata atatgcagcg
    23101 aaggagactg gttataaggc agatcaaaca gttcaagttg cgaaaatagc catcccgtca
    23161 ccccactcac ttcaggacgg ataaaactaa acttcagcgg atagagcaaa aaaccgcagc
    23221 aggccattac cgttgccaga ataagccggt ggacaagtcg gcgctgttcg aatgtcgtgc
    23281 tacaaacgaa cagcgaaaat ccatataaaa gatccagact ccagtaagga acaatagtcc
    23341 agggaagaaa agggatcgcc gtttcccaac cgaatacctg actggggata tcatggctgt
    23401 taaggtcctg aaccgcggtg aactgattaa gagatccata ggtgaagaag aaaaacgggg
    23461 ccagcaacaa taaccagcca gcgccttgta gcaatacgtt ccgacgttca gttatcacga
    23521 cgcaccgcca tcgaaaccgt aaaaataccc cactcatcaa tccgttgtgt gcatttatca
    23581 aatccggcat cacgcacgag tgaatccatc tccccttgcg aacgcacgcg cattacccac
    23641 ggtttaccat ctttatgact ggttaacacc ccggcaatca tctccagttg agggtgccac
    23701 ggctgcccgg tgtagatgag aatgccgccc ggttcgatgg cattggcaag acctgcgagt
    23761 gagtttttta cctgctcatt ttcgggaaaa agctcataca ggccagagac aatcgccagc
    23821 gtaggccgcg gagttaacgc gctgagttcc tccgggttaa acgcatcgcc ctgttcaaaa
    23881 cgcacccgcc cagacattcc ccgttgggca atcatcgctt gcccctgtgc aacatttaac
    23941 tcactgtaat cacgtaacaa aatatcgctt acggcaggct cgtttgccag cgcatccagt
    24001 acatagcgcc catgccctgc ggcaatgtca accacgcgga cggctaaacc tttggcgtgg
    24061 agatcggcaa cggcttgttt aatcagtatt tgtaaatggg ttttacgctg gcgaataccg
    24121 cgccagccaa cactgttcag gtagattttg tcaattaagc gcccgaatgc gttactgcct
    24181 tgcggttgat tttgatagac ataatccagg gaactgcctg aatcaaagcc ggtgctcatt
    24241 ccgagatgca ggcccgcaga gtgcgccccg aacaatttca tcgcctttcg cataaagcga
    24301 tacgccaaat caaccggcga taatggcacg ggtccacctg aaaggagccg ccagcgatcc
    24361 gctgatggtc cagtgcggtc ttcatgctga taatcaaatt tttgtgactt gttagcatat
    24421 aaacgactaa taaagctttg cattttttca aatgcctgcg ccctgttctc ttcacccaac
    24481 gtgtcgtgat aaaagcctgg cagcagatgc agctctttca gagggctacg taatctctga
    24541 taaaaatcaa tctgcggttg gcgatgcacc acatagtcat cgcctgatat cagaagttgc
    24601 gtggggagcg taatcgccgc cgcatctcta ataatacgtt cagacgtttt gtagagatcg
    24661 agcaagatat taacggcaat cgcccgtgtg atcagcggat cattattgaa actcgccacc
    24721 cgttgccgat cgtgggtcaa atagcgtcct ttcacatagg aattaataaa aaacagacca
    24781 cgcagacgat gccataacgc cagcgcagga cgtgccagcg gtacatacaa tttaacctta
    24841 aaggccggag aagccagcac cagcccgcga attgcaggcg cataatcatg aacccatgtg
    24901 gcaaccagca ctgcgccgac gctttgcgcg atcactacca cctcatccag tccgacctgg
    24961 ctatcgctgg cagcaaaacg aacaaattca tcgacatcct gcactgaacg cgcaagagat
    25021 gggctataac cacgcggccc cgaagtttgt ccatgccctc gggcatccca ggcataaaaa
    25081 acagtatctg gcatcgccag ttcatcaacg agatgttgta gacgaccaga atgttcatga
    25141 ccgcgatgaa ataagacgat gaccttttta gccccgggct gtaaagcggg ccagtgccga
    25201 taaaacaacg ctgtattatc actgctggta aaaaaatgtt ccccatggat gcgtgaattt
    25261 tccattttct ctattttcct tcagtaacag cagtagcgaa taaaataaaa aaataccgga
    25321 gcggtgaaaa ttaatgagtc aatcctgtca aggagtccgc catgtcctgg taatagtttc
    25381 ccactatctt taacaccaat atctcgtttg atggctgaca tcacgacatc gccgcaaaaa
    25441 ccactaatac caattaacaa acccgctaat aatgcctgta atgtatttag cggtgtcagc
    25501 aacggtccaa taatcagtga cgcaatcatg gtggtgatta cgccccccac cagaccttcc
    25561 agcgtttttc ccgggctgac tttggggacc acttttcttc tgccgcagga tttcccccat
    25621 aaatactgtg caatatcgtt tgactcggtt aatgccaaaa gaaataacac cagtaacgcc
    25681 ccgccctgga tatttgtggt tggcaacatt aataaccagg cggcatgact caaggcgaaa
    25741 acggtcatta tccagcccca aaaaatggcg ctcacggtat gcagaaaacc ggaggggtcc
    25801 cccataaaaa cttgcccggt ggctaatatt agaaacccag ccaaaggtat aaataataga
    25861 tacagctcaa agcaattaaa accgataaac aaatagttaa gaggaataac ccaatataac
    25921 caccgcggaa aatgtacaga tataagcgta caatattctt tcaatgccag aaaacttatc
    25981 agcgcaaaaa acgtcaacgt catccagcgt ggacctgaaa tcaccagtga aaaacaaatg
    26041 acgatgcccc accaggtacg aatacgtaat gtaacttcat cctcttttct ctccggtaac
    26101 cgccatagca gaaaatggtt tatcagcgtt gctaaaatta acaacgcgaa aagtgttgcc
    26161 agagattttt ccagcattat ctttcagcgc tcataagaag cacactccga caacgattaa
    26221 tcgcagtcca gagaagcaga attgaagcaa tgctccataa aagattattc cactgcatcc
    26281 actgtggata aatggcaacg gccagacccc acagaccaaa tattaacgcg cgatcgcttt
    26341 tgccaaatgg accggcataa ctgcgaacac cgttaatcgt ctgcgcgagt aaaccgcaaa
    26401 actcggtcaa tatggtgcaa aataacatga gtatcacgag tgatgcgtta ctttccggta
    26461 aaaataaaaa tggtaaatag agcgcaatat cggaaataac atcaccagtc tcattcaaaa
    26521 ttgccccaag ccgtgtttgc tgattgcact cacgcgccaa catgccatcc agcgcattga
    26581 gcgccatacg gataaaaagc acgatgggca atagcataaa gaggatgggt tgtgccacca
    26641 acatcaacag caatccggta agaagagaaa gcgccagtgc agcaagagtg atgtgattcg
    26701 ctgtaacgtg gtgcttgtaa agccaaaaca tcgtcggcct taacagcgac tgaaagagcg
    26761 gttttatctg gtagagcgtc attatcaatc cttgataatt cggtttgcgc tatggtgcat
    26821 caattcccct gacggagata tcgcaacacg ccgcttcatt aaaaattcag aatatatcaa
    26881 ggtattgggg actcactgtc aggaataatt atccccaatt aatcaaaggg ttaagaaata
    26941 ttacgttgtg gatcaataca actcatcaag ttttctcatc gcttgttccc gtattgcgcg
    27001 cgtcttaccg ttaagcgcca gctggcggac taaatccagt tcagggttaa caagctctaa
    27061 cgccagcaaa ggatcacgtt tcagcctcgc ctttaataca tcaactggaa aacggggatt
    27121 attcatcgcc acaatccacc actcgggatc aatttctgcc gccagtactg caggcggtgt
    27181 gtggcggttg ttaagtgcgc cgaacttgaa aaatgtgaga ttgcttttcg ccagtgtact
    27241 ccaccatata cgatccggtt gttttgccag tacatccagt tgtgctaacg catcggggtc
    27301 gagtgcataa tagacgcgta atgcttcatc aacctgcgca cgggagctgt tttcctgcct
    27361 cagaaaaaac ttcgcccaaa tatcattgcg tggcgtattt tgctgtaatt ccttgaggac
    27421 aacagcgggt aaattatccc aactaactaa tgcactctct aaaaagtccc agtcttccaa
    27481 acgtatcagg gcggtggcat ttaatagcat ctgatcatca acttcaccgt ggctgataag
    27541 ctcagacaaa atcatatcag cgctggtttc atacgcagct gaatctgctt tcggattatt
    27601 tttccgatgc gataaagcac gttgccaaat tttttgtttc atcgacgtgc tcagatactg
    27661 atttttcgcc agattactcc acccctcagg atcgttgaaa cgtgtcaata accgttgtag
    27721 agcagcgtca gagtgaatta cctgcatatc ccgacagcaa aagtaggcag gggatttttt
    27781 ctccatcctt ttaacaaaat taaactgtaa gttatctggc aatgctaaaa ataacacacg
    27841 tattagatca tcgtcattct cgaatttttg gtataaatag gctgcaactt tgtcactctc
    27901 ttgtggcgtc atccgcaata attgaccatg gtgtttcatc tctgccagct tgaacgctaa
    27961 gcttgtgaca acattgtttg atgaatcgtt cgccaactgc attgcctgat tgtaggtgag
    28021 ttgtcctcca gcaccgacct gacgaataga ggaatcatca ctgcggatat agccatcaat
    28081 aatggattca ggcaataacc acgcatcttt ataaagctcc tgaatgatca tatcccaatc
    28141 tcttttcgcc agagtcatta cgatatccag agggagattt tcacgtagcg caagattcaa
    28201 cggaccgaca ccatcaggta gacgactcat tcgcgtcatt atttcgacag agagcggttc
    28261 acggtcgagc aaccccagaa caacaggctg gtaacccaat tgctgatcgg tcaactccag
    28321 aatctcattg tcaggcatat atctcgccat actttcaatc gtttgttcat cattgctatt
    28381 aataagttgt ttgcgtaggt tagctgaaat tcctttaagc cttgggagag ttcgcattat
    28441 ggcaagatca aatgtcgcaa tatctcgtcc ttcaaatgca gcaacgttgc cttcatccag
    28501 catttgcaat tcctgcacta actcaggaga aggcttaggg cgaaacagcg cttctttttt
    28561 catctgatta cagacattcc ctgccatgcg aatattgctt cgcttaaaaa tttctttcag
    28621 agcatcttca tcatttatta gaccccaata atatgcatca ggatttatta tcatcagcgc
    28681 ttttttgaca ttttcagtgt tatttttgtc actcgcacgg accatgaatt cactatttgt
    28741 cgaagacgca acaacaggtg caattatgtc attgataaat attccacgcg cattaatact
    28801 ccccttaatc gaagacggat tatcgtaata tgagtcgata aataccagtc cacttgcatt
    28861 tatcgaaccg gtaattaaca gtggtgaatc cgtaactata ttcgttgccg tcacgttacc
    28921 gagaataacc catggattag aaagagagaa agtggaaata ttacctttga ctgtcaaatt
    28981 accaagaaca atcaattttt gattactctc tatattacct tctaccacta aatcgccacg
    29041 atgcactttc cagtccgatg cagataaacc gaaggggaca agattcacgc tactgttgat
    29101 agcttgttca acttgttgtt tatcttcagc ggtagccgca gaaaaaaatg gttcaatatc
    29161 aactgtattt gcaacacatc gaagttccgc ctgtactgga agactcagca taaacagggg
    29221 aagtaaacct atccagccgc aaatcctgga cttaaacata tgcactccat gccattaaaa
    29281 aagaaacaaa agggaaatgc atctttccct tttgtatatt cgttattctc gcgaaatacc
    29341 atccaacgta gacaacactt cctcagaaag atgcaatttt tcagcagcca tattctcccg
    29401 taaatgcgca acagaagacg tccctgggat cagcaaaata ttcggtgaac gttgtaacag
    29461 ccacgccagc gccacctgca tgggtgttgc acccaggctc gcagcaacat ccgacagcgt
    29521 agaggattgc agcggtgtaa agcccccgag cgggaagaac ggcacgtagg caatgccatc
    29581 gtgggccaaa gcatcaatca ttgcatcatc agcacggtgc gcgatgttgt attcgttttg
    29641 cacacagaca atttcggcaa tcttgcgcgc ctctgcaacc tgcgtcggtg tgacgttgct
    29701 caggccaata tgttttacca ggccttgttg ttgcatctct gccagcacgg tcaggctggc
    29761 ctcaatcgat ccttccgctg ggccatgacc atctcccatc ataacgcgca ggttaaccac
    29821 atccagtacg tccaggccga gattacgtag attatcgtgc accgcttttt gcaactctgc
    29881 cggagaaaag gcgggcaacc aggaagcgtc ctctccacgc cgcgcaccaa ttttagtgac
    29941 aattgtcagg tcgtcagagt aaggataaag cgcttcgcgg ataatctgat tggtgacgtg
    30001 cggaccataa aagtcgctgg tatcaatgtg attgacgccc aatgccagcg cctcgcgcag
    30061 cacagttata gcgacgtggc gatctcttgg gggaccaaaa accccaggac ctgccagttg
    30121 catcgcgcca taaccaagac ggttaacgga ttttgtaccg agagtaaatg tattgctgct
    30181 catcgcaccc tgccctgtaa taaaacggat taagaaaaat cagtatagaa acgaaaagag
    30241 ataaataatg cgagggcgta aacaagatta ctggatgagt tgctctattg tcctgaaaaa
    30301 cacaaaacaa aacgcgtaaa gcgccgcatc cggcaataaa aaaaccgcaa cctgtatcag
    30361 cgttgcggtt taggtatcac atgctaaaaa ttagaatgtt cagtatctgg acgttactgt
    30421 ttcggtcgca gattctcatc aaataccaga cgtttcctgt tcggttcaac ttcccgcaat
    30481 ggctcaactt gatgcaacgt ctgcttacaa cgagtcacca gcacctgata ctcatgcgta
    30541 ccctgctttt tccatgccag ctcctgctca ctgagttcac gaatcgcttt cgccgggctg
    30601 ccgacaatca ggtaattggc aggcatttct gctttggctt tcacaaatgc ggatgcgcca
    30661 acaatgctgt tctcgccaat caccgcgccg tccatcacta ccgcgttcat tcccactaat
    30721 gcattgcggc ggataatgca accatgaagg atagcgctat gaccaatatg tccatcttct
    30781 tccacgacag tatcctgctc gggaaaaccg tgcataacgc aattatcctg aatgttcgcg
    30841 ccatctttca ccacgatacg accaaaatcg ccacgcaggc tggcatttgg cccaacgtaa
    30901 acgcccttgc ctagaataac atcgccgatc aataccgctg tcggatggac aaaactctct
    30961 tctggcacaa ccggagtcag accatctatc tggtaaattg gcatatcgcc tcctgttcaa
    31021 tattcaaacc gccaaaacgt tgaaaataca ggcttcccgg cgcaggcagt tcaccgaccg
    31081 aggtttcacc tttttcacta acgaacgcta aagcagcagg cgctacgcgc tgataaatgt
    31141 tgatacacag ctgacgcgcc gtatgccctg cccagtgcgc cggaagcaac tcctctggca
    31201 acaatgggtc tttaaggacg acacggcgat aaaaatggat cagtaaaagc tgaatatgaa
    31261 atgcacgctc cggggttaac tcgtctgctg cctcttttaa cagcggcacc agcgggcgga
    31321 atgactgaat aaaggtttcg tacatggcat tttgttcagt taaatgccag cactcttcca
    31381 ctctggcacg cagtgctgca cgagaaagcg ccagtggtat ttgcgcttca aaacaaatca
    31441 cgttatccgc cacccccgct tcatgcaaaa gtgtctgtac atccgccagt ttttgcgacg
    31501 gcgatgccat caggctgggt gccagtgcgc caaaaccttg ccagatcaac tgctttttga
    31561 catcagccag cgttgattta tctaaacctt ccgagagcaa caggagccat ttaccatccc
    31621 atgcaggttg ctctgcgcga taaattttac tttctgcccg tcgcgtcagg cgcaagcctt
    31681 tatcactgag gctatagaaa ctgcgtcgcc cgatgcggga aacatccagc cagccttctt
    31741 tattaagacg aaacaaagcg gtgcgcacga aacgctcacc aaatcccagc ccttccagca
    31801 aagcagccag actacccagc cagatttcac caccacgatg ggaaagcgaa tcaccataca
    31861 gagaggagat caaagatgtg ccactgaccg gaacagcgtt tacagcatgt tggataaaag
    31921 taacaagttt actcattcga ttcagtctgt tgttattgtt gtgatttatg aatcatagca
    31981 caggtcgtat cgacttctgg ccctggcgct ttacctcacc agggccagac gacacgtcag
    32041 gcaccaacaa tattgcgcag atcaaacacc cgacacgcct tgccttctga acgcgggata
    32101 ctgccacagt taacgatcat cacatcggta gagatcccca ccatcgactt aatccgatgg
    32161 cgcagctggt ggcacacctg gcaacgctgc tcatgcgtca atgtcaggct gctttctttc
    32221 aactccactt tcacagaaag tgaatcaaga tgcccccggc ggttcacctc cagttggtaa
    32281 tggggcgata aatgttcgaa tttgacaatc tcttcttcca gttgtgacgg gaagacattt
    32341 acaccgcgaa tgatcaacat gtcatcgctg cgtccgctga tgcgatccat gcggcgcata
    32401 gtccgcgccg ttcctggtaa cagtcgggtt aaatcacgcg tgcggtaacg aatgaccggc
    32461 aatgcttctt tggtcagcgt ggtgaataac agttcgccat gttcaccatc ggcaagcggt
    32521 gtgccgtcat gaggattaac aatttcagga tagaaatgat cttcccaaat ggttgggccg
    32581 tcggtagttt ccagacactc catcgccccc cccggcccca tcacttctga caggccataa
    32641 atatccagtg cggtgatccc cagacgccgt tcaatctctt tacgcatggc ctgtgtccac
    32701 ggctcagcac caaacactcc aacccgcagc gagcaaccgc tggcatcacc gcccaactgc
    32761 cgctccagct cttcaatcag gttaaggcaa taagatggcg taaccatgat catatctggt
    32821 tgaaaatcgc ggatcagttg cgcttgtttt tccgtctgcc caccagacat cgggatcacc
    32881 gtagcgccca gacgttcggc accatagtgc gcgcccagcc caccagtaaa caggccgtaa
    32941 ccataggcaa catgaatttt gtctttgggc gtgccacctg ccgcacgcaa ggaacgcgct
    33001 acaatattgg cccagttatc aatatcgttt tgcgtatagc cgacaactgt cggtttacct
    33061 gtggttcccg aagaagcatg aatgcgcacc acttgttcca tcggcacggc aaaggtgtcg
    33121 aagggatagt tgtcgcgcag atcctgtttg gtggtacagg gaaatttacg cagatctgaa
    33181 agttccctga aatcatcagg atgtacgcct gctgcgtcaa atttgcgccg atacatcggg
    33241 acattttcat acgcgtgttt gagcgtccat ttcagacgtt gtgtttgcaa cgcctgtaac
    33301 tcatcaacag acgcggtttc aatcgggtca agctttgtat tggttatcat tgtagggtac
    33361 tcgcaggttg atatgctcaa acacgctcca gaatcatggc gatgccctga ccgacaccga
    33421 tgcacatggt gcacaatgcg taacgaccgt tacgccgatg cagctcatgg ctggcagcca
    33481 gtgccaggcg ggcaccactc attcccaacg gatggcctaa ggcgatagcg cctccgttgg
    33541 ggttaacatg tggggcatca tcaggcagcc ccaattcgcg tagtacaccc aacgcctggg
    33601 ccgcgaacgc ttcgttcagt tcaatcacgt ccatatcgtg aatactcagc cccgcgcgtt
    33661 ccagcacccg gcgagttgca ggcaccggac caagccccat caggcgcggt tccaccccgg
    33721 cggttgccat ggctacgatt cgcgcccgcg gtgtcagtcc ttgcgctgct gccatctgtt
    33781 cactggcaat aatcaacgca gcggctccgt cattcacccc ggaggcattg cctgcggtaa
    33841 tcaccccatt ggcacgaaat ggtgctttta acccacgtaa ctgttccagc gtcgtttccg
    33901 ggcgcagatg ctcatcatgt tgtatttctg ttacaacgcc tttcttgttt ttcaacacaa
    33961 ccggaacaat ctcctcagcc agaatgcctg aggattgcgc ttttgccgta cgttgctgac
    34021 tgcgtagcgc aaaactatct tgatcttctc gcgatatttt taacagttca gctacattct
    34081 ctgccgtttc cggcatgctg tcagttccaa attgttgagc catgagcggg ttcacaaatc
    34141 gccagccaat agtggtatca aacatctcag cctgacgaga aaatgcactg gttgccttgc
    34201 ccataacaaa cggtgcccga gacattgact ccacgccacc agcgatcagc aaatcgccat
    34261 cgcccgcttt aatcgcccgt gcggcgaacc ccagtgcgtc cagcccggaa ccacacaagc
    34321 ggttaatggt tgtgccggaa acactatgcg gcagccccgc cagtaacgtc gccatccggg
    34381 ctacgttacg gttgtcttct cccgcctgat tagcacagcc gaggatcaca tcatcgatac
    34441 actccgcatc gagacgcggg tttcgcacca gcagctcccg caaagggata gcagccagat
    34501 cgtcagcccg aacacctgat aatgccccac catagcggcc aattggcgta cgaattccgt
    34561 cacagataaa ggcttcacgc atcaggcttc tcctgtaatg gtgccgccga tgcggtgaga
    34621 tttaccgcga aacagcgcaa ccgttttttg ttgttggtta acaatttcga tgtcgtaaac
    34681 accggtttgc ttgccctgat gacgtacctg cgcagtagca gttaaggtgt ctccggcaaa
    34741 gcctggacgc aaaaaatcaa tcgtgcaggc agaagcgacg gctgccagcc cctggctatt
    34801 gcaggcgtag gcaaaggcag tatcagccag tgaaaatagc tgcccgccgt ggcaactttg
    34861 atgaccgtta agcatttgtg cagtgacggt catggtcact acagcaaagc cttcatccat
    34921 tgagataatg tcgatgccaa gcgctttggc gcaggcatcg ttctcataca ttgcatgggc
    34981 attttgccag gccttatgac tcataaccgc tctccagaag cgctcgttgg cgcagcaatg
    35041 aacatgggcg atagcgttct tcgccatagt gatgttgtag attttccagg agccttaata
    35101 ttcgctgcca gccaagttgc gctccccagg caagcgggcc atatggataa ttcaccccaa
    35161 gacgcatggc ggtatcgata tcctgttcag aggccacgcc tttttgcagc gcatcaaggg
    35221 cttcattgat gatcattgct accgttcgcc aaatcagcat tcctgggtaa tctgcaattt
    35281 gcagcactgt tttgccctgc tgttgcaggt aataaatggc cttgcgggtc gctgagtccg
    35341 ggttcaccgc tgccgcagca atggtcacca ccttgcccgc cattttgtcg accactacca
    35401 ccgggcgtgc cagtcgtatt gccagcgcct gtgccgtctc gccttgtgtc tcaatcaata
    35461 aaacatcgtc aatttccgtg acgccgtcac ttttcttttc tactttcatt gggctaaaac
    35521 tgtcgcttac cgcttccagg ccaacaactg cctcacgttc cgcgcgccag tcgtacacgc
    35581 ccagcccact tttcttgccc aaccgtccac caatcaccag ttcctgttgc accagcgaag
    35641 gtaaaaaacg acgctcctgc cagaaagcgt taaacaccga acaggtgaca gcaaaattga
    35701 cgtcctgacc aatcagatcg gttaattcca gcggccccat cgggaaacca gcgccatcgc
    35761 gaagtgcagc gtcaatcact tctggtgcag caacctgctc ttccagtgcc cgccaggcct
    35821 cggaataata aggacgcgcc acacggttaa cgataaatcc aggggtcgaa tgacagcgca
    35881 caggctgctt accccaactc aacgttagtt cacacaactg ctcaacaact tccgctgccg
    35941 ttgccagccc actgaccacc tccaccaact tcatcaccgg tgccgggtta aaaaaatgca
    36001 gccccgcaac acgttcagga ttttttacct ccgcagcaat cgcggttata gagattgacg
    36061 aagtgttagt ggtcaatagc gtttgtggcg ggcaaacttc cgccagctgt gcaaagagcg
    36121 cttttttgac ttccagacgt tcagacgccg cttcaatgac caggtccgca gctgccagcg
    36181 cgtgaatatc ggtcaccggg atcaggcgtt tcaatgtgcg ttcacaggtt tcagcagtca
    36241 gttttccccg cgtcacgcgt gaatttagcc gcgcgtgtat cccgtcgatt gcgcgggtca
    36301 gcgcttcagc agaaatgtca tacagtaaaa cctgatgtcc atgactggcg gcaacttcag
    36361 caatgccagc ccccatggtg ccgctcccaa tcactgccac agtttgcaca tttatcatca
    36421 tgctatttcc ccgtgaactg cggtgagcgt ttagccagga acgcactgac accttcacga
    36481 taatctgcgc tccgtccggc aagtcgctga tagtcgcgtt ccagatccag ttgcgtatcg
    36541 agcgtattgg tttcagcgct atttatcgct tgcttgataa gtccaagacc aaatgtcggt
    36601 tgtgtcgcca gatgccgtgc cagctgttgc gcggtatctg ccagcgtttc atcatcaaca
    36661 acctgccaga tcatccccca ttcgtgcgcc tgttcagcac tcagttgatt ccccagcagt
    36721 gccagcccca tggcgcgcgc tcgcccagca acgcgtggca gtaaccaggt tccaccgcaa
    36781 tcgggtatta agcctaactt actaaacgcc atgacgaatt ttgctgaacg ggcagcaata
    36841 acgatgtcgc accccagtgc cagtgttgcg cctgcccctg ccgccacgcc attgactgca
    36901 cagatcaccg gttttggcag ttttgccagg cgacgtacca gtgggttata gaaacgttca
    36961 actgacatcc ctaaatccgg tgcggggcct gtgggatcaa cgttacgatc gttaagatcc
    37021 tgaccagcac aaaatccgcg cccggcacct gtaagtaaca ggcaacggat agtgtcgtcg
    37081 cgctcgacct gtttcaggca ctctgccagt tgtgcgtgca tctcatcatt aaaactgttc
    37141 aggcgttccg ggcggttgag cgttagtgtc atcacgccct tttctacatg actgaggatg
    37201 aattccatca ttagcgtcct ttaaagtcgg gcgtgcgttt ttgtaagaaa gcggagatgc
    37261 cttcatgacg atcttctgtt gccgccagca aggtgaataa ctgtcgctcc tgggcaagtc
    37321 cggcttgcaa agccacttcc tgcgactggc gcagcgcttg ctttgccgct tgtaaggcca
    37381 acggcgagtg acgtgccatt ttcgatgcca gctgtaaggc gtattcgagg gttaaatcgc
    37441 tggggaagac gtcgctaacc agcccggcct gctgtgcttg ctgggcggtg atactttctc
    37501 cgctcagcac cattttgctg gctaacgatt taccgacact acggattaaa cgttgcgttc
    37561 cgcctgcgcc aggcatgatg ccgagagtga tttccggcaa cccaaaacgc gcgttctctc
    37621 cggcaaccac cacatcgcac aacaatgcca gttcgcaacc cgccccaagc gcgtaaccat
    37681 tgacggctgc gataagtggt ttattaaagg cctgcaatcg cgcccatagc tgcggacgtg
    37741 tatcgtttaa ggtggccgcg agatcttttt ctgccatttc gttgagatcg gccccagcgg
    37801 caaaaaagcg tgcattaccg gtaatcacac agaccgaaat gctggtatcg gtagccgcag
    37861 cttccagttc atttaccagt tgcatcagca gggcattatt tagcgcatta cgtgcggcgg
    37921 gacggttaag ggtcagcaac aatactcgtt gctgacggct gacgatcagt tcgctcatgc
    37981 catccccttc gcgtcaaagt caaccaccac atcgctggtc agcggcagtg cctggcaact
    38041 caacacataa cctgcggcca gttcatccgg ttccagactg taattggttt ccatcgccac
    38101 tttgccacgc agcactttgc atttgcaggt cgcacagacg ccgcctttgc aggcataggg
    38161 cagatcagcc ccctggcgca atgccgcatc gagaatgctt tcatcgtcgg cattaagcac
    38221 gatttcccga tcccgcccat cctgacgtac agtcactttt tgtccgtcac tttgcacgtt
    38281 aacgctacgt ttgacgcgcg tgccaggcgt attaaaccgc tccagatgaa tggttttatc
    38341 tggcattccc agtgctttta aggcggcttc cgtctcatcc atcatcgccg ccggaccaca
    38401 aataaatgcc tcatcataaa gacgaaaatt aatgagcgag gccccaagtg actgtaatct
    38461 ttcaccgtca atacgcccgt gaagcagatc gctgtcgagg gtttcctgac tgaaaatgca
    38521 caacaactgt aaacgctgag gatatttgtc tttcaggtct gccagtgcct ggcgaaacat
    38581 catgctctgg ctggtacggt taccgtagat cagggtgaac tgactttcag gctcggtttg
    38641 taaagtggtg gcgataatcg ccagcattgg cgtaatacct gatcctgctg caatagccag
    38701 atagcggccc tggcgttcgg cctggggctg atagccgaaa tgcccctgcg gcaccatgac
    38761 ctccagcgtc ataccctggc ggatgtgttc gcgggcatag cgggagaaac gtccgccttc
    38821 aatggctttc accgccacac taatttcgcc aggcaggtag ctgcggcaaa tggagtaaca
    38881 acggcgtaat tcttcaccat caagactggc ttttaaggtc aaatgttgac cggggcgaaa
    38941 gcgatacgcc tcctgcaaag gctggggcac cgcaaaggta atggtcaccg catcacgggt
    39001 ttccggctcg acttttgcca ctgttaagga atgaaacgtt gtcatggcat cctcaaatac
    39061 atttgaaata atcgaaaggt tcgcggcaac tatcgcagcg gtacaatgct ttgcaggccg
    39121 tggaaccaaa ttcactgata agtgtggtat ggacgctggc gcagcgcggg caacgtactt
    39181 ctggcggcaa atgggcatgg caactgtgtc cggcgggcgg gctaatgcca tactgtcgca
    39241 gacgctcacg ggcatcgggg gtcatccagt cggtggtcca tgccgggtcg agttgcagca
    39301 caacctgaac gggggtaaac ccgtgggttg tcattgcctc acgtatcgcg ccaatcaaat
    39361 gttccgttgc cggacaaccg gaataagtcg gcgtaaagcc gatcacccat ccttctccca
    39421 tctgtgtcac attacgcacc atgcctaaat cggtaatggt cagcaccggg atctccgggt
    39481 ccgggatctg gctgagtagc gcccatatct catgaacctg tggcggtgca atggtggcca
    39541 gacgttgcat acccatctcc tctgttacca ttgctgaccg ggcaagacac gctggagata
    39601 ctgcatttct gccagcatgg gtccaaggtg ttcggtatgc agtccttttt taccgccagt
    39661 gcgatacgcc tgctcttgcg gtacgttcaa tgtggcttcg ttgattccgg caaaaacttc
    39721 ggcttcccac gctgcgcgta aagtgcgtgg atcaaccgca ataccctctt cactcagtgc
    39781 aatatcaatc tcgtcggcat cgaacagttc ggcagtaaaa cgccacaact tgtcgatggc
    39841 ctgctgcatc ttttgccctg atacgtcaat accattgccc agtcgctcca gccagccaca
    39901 actaaaacgc aggtgataac gcgcttcttt aattgctttg gcagaaatcg ccgccagttg
    39961 cggatcacgg ctttccatca gacgggtaaa gagcgccaca tgccatgcat cgatgaaata
    40021 ctggcgtgca atggtgtcgg caaaattgcc gtttggttgt tcaaccaaca ataagttgct
    40081 gaactggcgc tcgtctcggg tgaaggccag ggtatcttca tcaccttctc ccgctaattc
    40141 agcggcatac gataagaagt tacgtgcctg acctaataaa tcgaggccaa tgtttgccag
    40201 tgcgagatcg atttccagtt ccggtgcgtg accgcaccat tcccccaacc gctgggagag
    40261 caccagatag ttatcgccca ggcgcaaggt gtaagccgtt aactgattca tttccgacct
    40321 cacatgtgct caatgccatc agggatggtg taaaacgttg gatggcgata gaccttgctt
    40381 tcagccggat caaaaaattc accgcgttct tccggttgcg aggcaacaat ttcactcgcc
    40441 ttcaccaccc aaattgaaca tccttcgcta cgacgggtgt aagcatcacg ggcattttcc
    40501 agtgccatcc gctcatcggc agcatgtaaa ctgccaacat ggcggtgtga taagccctgt
    40561 ttgccacgca cgaaaacttc gtataacggc cagtaaacat tactcatttt gaatctcctt
    40621 atgcgacctt acgggcaagt tgtttttgtg catgggccag cgctgcttcc cgtacccagg
    40681 taccttcttc ccaggcttta cgtttggcgt cgagcctctc ctgattacaa attccgcgac
    40741 cgttaattac ttcgttaaac tcctgccagt cgatctcacc aaagcggtag tgaccgcttt
    40801 cagtgtcaaa atgcagatcc gggtcaggaa cggtcatacc gagcatttca acctgtggaa
    40861 cggtgttgtc cacgaaacgc tggcggagtt cgtcgttggt gaaacgtttg attttccagg
    40921 cgagacttct ggcgctgttt ggcgagttat catcgtttgg cccgaacatc attaaggctg
    40981 gccaccagaa acggttaatg gcgtcttgca acatctgctt ttgagcttcg ctgccctgcg
    41041 ccagtgccat gcaggcttca aacccctgac gctggtgaaa actctcttct ttgcagattt
    41101 tcaccattgc tctggcatac gggccataag aagttcggca taacgccacc tgattgacga
    41161 tcgctgcgcc atccaccagc cagccgataa cgccgatatc agcccaactc aatgtcggat
    41221 aattaaagat ggaggaatat ttcatccgtc cgtcgagcat tttttggtag atgtcttctc
    41281 tggcacagcc cagtgtttcg gcggcgctat agagatacag accatgaccg gcttcatcct
    41341 gcactttggc taacagaatg gctttacgcc gcaaggttgg tgcgcgagtg atccagttac
    41401 cttcaggcaa cataccaaca atctcggagt gcgcatgctg cccaatctgg cggatcaatg
    41461 tctttcggta agcatcgggc atccagtcct gcggctcgat agccgtttcc tgggctatcc
    41521 gttgctcaaa gcgttcttct tgggtcacgt catcaccttt acgattccag ttttcgtgaa
    41581 ttaattaaaa acacgaatca cgttgtttta tgaaagttac acaatggtta actaaagtca
    41641 caaattttgt atacttgaac tgtgacagat ttcgcactct attttgataa ctcattattt
    41701 atcagtgcat taatgttttt agtagttgat atacagatca cgcagttaac atttcgttaa
    41761 aagatccctt gctttttatg attcgcgatt taactattag caacagaaat gtgaaacatc
    41821 tggagagtag cgatgcagca gttagccagt ttcttatccg gtacctggca gtctggccgg
    41881 ggccgtagcc gtttgattca ccacgctatt agcggcgagg cgttatggga agtgaccagt
    41941 gaaggtcttg atatggcggc tgcccgccag tttgccattg aaaaaggtgc ccccgccctc
    42001 cgcgcgatga cctttatcga acgtgcggcg atgcttaaag cggtcgctaa acatctgctg
    42061 agtgaaaaag agcgtttcta tgctctttct gcgcaaacag gcgcaacgcg ggcagacagt
    42121 tgggttgata ttgaaggtgg cattgggacg ttatttactt acgccagcct cggtagccgg
    42181 gagctgcctg acgatacgct gtggccggaa gatgaattga tccccttatc gaaagaaggt
    42241 ggatttgccg cgcgccatgt actgacctcg aagtcaggcg tggcagtgca tattaacgcc
    42301 tttaacttcc cctgctgggg aatgctggaa aagctggcac caacgtggct gggcggaatg
    42361 ccagccatca tcaaaccagc taccgcgacg gcccaactga ctcaggcgat ggtgaaatca
    42421 attgtcgata gtggtcttgt tcccgaaggc gcaattagtc tgatctgcgg tagtgctggc
    42481 gacttgttgg atcatctgga cagccaggat gtggtgactt tcacggggtc agcggcgacc
    42541 ggacagatgc tgcgagttca gccaaatatc gtcgccaaat ctatcccctt cactatggaa
    42601 gctgattccc tgaactgctg cgtactgggc gaagatgtca ccccggatca accggagttt
    42661 gcgctgttta ttcgtgaagt tgtgcgtgag atgaccacaa aagccgggca aaaatgtacg
    42721 gcaatccggc ggattattgt gccgcaggca ttggttaatg ctgtcagtga tgctctggtt
    42781 gcgcgattac agaaagtcgt ggtcggtgat cctgctcagg aaggcgtgaa aatgggcgca
    42841 ctggtaaatg ctgagcagcg tgccgatgtg caggaaaaag tgaacatatt gctggctgca
    42901 ggatgcgaga ttcgcctcgg tggtcaggcg gatttatctg ctgcgggtgc cttcttcccg
    42961 ccaaccttat tgtactgtcc gcagccggat gaaacaccgg cggtacatgc aacagaagcc
    43021 tttggccctg tcgcaacgct aatgccagca caaaaccagc aacatgctct gcaactggct
    43081 tgtgcaggcg gcggtagcct tgcgggaacg ctggtgacgg ctgatccgca aattgcgcgt
    43141 cagtttattg ccgacgcggc acgtacgcat gggcgaattc agatcctcaa tgaagagtcg
    43201 gcaaaagaat ccaccgggca tggctcccca ctgccacaac tggtacatgg tgggcctggt
    43261 cgcgcaggag gcggtgaaga attaggtggt ttacgagcgg tgaaacatta catgcagcga
    43321 accgctgttc agggtagtcc gacgatgctt gcagctatca gtaaacagtg ggtgcgcggt
    43381 gcgaaagtcg aagaagatcg tattcatccg ttccgcaaat attttgagga gctacaacca
    43441 ggcgacagcc tgttgactcc ccgccgcaca atgacagagg ccgatattgt taactttgct
    43501 tgcctcagcg gcgatcattt ctatgcacat atggataaga ttgctgctgc cgaatctatt
    43561 ttcggtgagc gggtggtgca tgggtatttt gtgctttctg cggctgcggg tctgtttgtc
    43621 gatgccggtg ttggtccggt cattgctaac tacgggctgg aaagcttgcg ttttatcgaa
    43681 cccgtaaagc caggcgatac catccaggtg cgtctcacct gtaagcgcaa gacgctgaaa
    43741 aaacagcgta gcgcagaaga aaaaccaaca ggtgtggtgg aatgggcggt agaggtattc
    43801 aatcagcatc aaaccccggt ggcgctgtat tcaattctga cgctggtggc caggcagcac
    43861 ggtgattttg tcgattaatc ggtgaatgaa gggcaacggc caatagttgc ccttttattt
    43921 cactaagttt tgtgacgttg tcacattatg catgatgtgt acatctattt tcagggcacc
    43981 cactgtatga aaagctggca cacctgccaa acaacctggc aggtgcaggc aatccccttt
    44041 gcatcagtac tgataatgtg aacctgacta aaccgcccac agagcgcggt tgctaacaag
    44101 aacacaacat ctgacgaggt taataatggg aagcccctct ctgtattctg cccgtaaaac
    44161 aaccctggcg ttggcagtcg ccttaagttt cgcctggcaa gcgccggtat ttgcccacgg
    44221 tggtgaagcg catatggtgc caatggatga aacgcttaaa gaatttggtg ccgatgtgca
    44281 gtgggacgac tacgcccaga tctttaccct gattaaagat ggcgcgtacg tgaaagtgaa
    44341 gcctggtgcg caaacagcaa ttgttaatgg tcaacctctg gcactgcaag tgccggtagt
    44401 gatgaaagac aataaggcct gggtttctga cacctttatt aacgatgttt tccagtccgg
    44461 tctggatcaa acctttcagg tagaaaagcg ccctcaccca cttaatgcgc taactgcgga
    44521 cgaaattaaa caggccgttg aaattgttaa agcttccgcg gacttcaaac ccaatacccg
    44581 ttttactgag atctccctgc taccgccaga taaagaagct gtctgggcgt ttgcgctgga
    44641 aaacaaaccg gttgaccagc cacgcaaagc cgacgtcatt atgctcgacg gcaaacatat
    44701 catcgaagcg gtggtggatc tgcaaaacaa caaactgctc tcctggcaac ccattaaaga
    44761 cgcccacggt atggtgttgc tggatgattt cgccagtgtg cagaacatta ttaacaacag
    44821 tgaagaattt gccgctgccg tgaagaaacg cggtattact gatgcgaaaa aagtgattac
    44881 cacgccgctg accgtaggtt atttcgatgg taaagatggc ctgaaacaag atgcccggtt
    44941 gctcaaagtc atcagctatc ttgatgtcgg tgatggcaac tactgggcac atcccatcga
    45001 aaacctggtg gcggtcgttg atttagaaca gaaaaaaatc gttaagattg aagaaggtcc
    45061 ggtagttccg gtgccaatga ccgcacgccc atttgatggc cgtgaccgcg ttgctccggc
    45121 agttaagcct atgcaaatca ttgagcctga aggtaaaaat tacaccatta ctggcgatat
    45181 gattcactgg cggaactggg attttcacct cagcatgaac tctcgcgtcg ggccgatgat
    45241 ctccaccgtg acttataacg acaatggcac caaacgcaaa gtcatgtacg aaggttctct
    45301 cggcggcatg attgtgcctt acggtgatcc tgatattggc tggtacttta aagcgtatct
    45361 ggactctggt gactacggta tgggcacgct aacctcacca attgctcgtg gtaaagatgc
    45421 cccgtctaac gcagtgctcc ttaatgaaac catcgccgac tacactggcg tgccgatgga
    45481 gatccctcgc gctatcgcgg tatttgaacg ttatgccggg cctgagtata agcatcagga
    45541 aatgggccag cccaacgtca gtaccgaacg ccgggagtta gtggtgcgct ggatcagtac
    45601 agtgggtaac tatgactaca tttttgactg gatcttccat gaaaacggca ctattggcat
    45661 tgatgccggt gctacgggca tcgaagcggt gaaaggtgtt aaagcgaaaa ccatgcacga
    45721 tgagacggcg aaagatgaca cgcgctacgg cacgcttatc gatcacaata tcgtgggtac
    45781 tacacaccaa catatttata atttccgcct cgatctggat gtagatggcg agaataacag
    45841 cctggtggcg atggacccag tggtaaaacc gaatactgcc ggtggcccac gcaccagtac
    45901 catgcaagtt aatcagtaca acatcggcaa tgaacaggat gccgcgcaga agtttgatcc
    45961 gggcacgatt cgtctgttga gtaacccgaa taaagagaac cgcatgggca atccagtttc
    46021 ctatcaaatt attccttatg caggtggtac tcacccggta gcaaaaggtg cccagttcgc
    46081 gccggacgag tggatctatc atcgtttaag ctttatggac aagcagcttt gggtaacgcg
    46141 ttatcatcct ggcgagcgtt tcccggaagg caaatatccg aaccgttcta ctcatgacac
    46201 cggtcttgga caatacagta aagataacga gtcgctggac aacaccgacg ccgttgtctg
    46261 gatgaccacc ggcaccacac atgtggcccg cgccgaagag tggccgatta tgccgaccga
    46321 atgggtacat actctgctta aaccatggaa cttctttgac gaaacgccaa cgctaggggc
    46381 gctgaagaaa gataagtgat tgtttcagac aaaaaaacgg caccaggtgc cgttttttac
    46441 ttatgagcga accagattaa taccgtacac acaccgactt agtttcacac caaccgtcca
    46501 gccagtcggg gccaaaatca cggcccgttc ctgactgctt catcccacca aacggtaagt
    46561 tagcgtcaat taaggtatgg ctgtttaccc acaccgtccc tgcctgtaag cgatcgctat
    46621 attccagagc ctgggagaga ttttgcgtcc agacactggc agttaagcca tattccgtgt
    46681 cgtttgccag ttgtaacgcc tcttctccat ccgctactcg caccaggttt accaccggac
    46741 caaacacctc ttcacgagtt aagcgcaatt tagcatcggg atttaccacc agcgttggcg
    46801 caacataata cccctctccg gctggtccat tcgacccgcg aatcagctct gcttgctgtg
    46861 cctgcgcatc gtcgaggaat gaacacactt tgtcgcagtg cgcacgagaa accaaagggt
    46921 taatctgtgc aacaggtgac atccccggtc ccacttgcaa cgattttacc gcctgctcaa
    46981 atccactaac cagcgtgtca aacaacggcg cttcaatata aattcgcgaa ctggcggcgc
    47041 atacttgccc ttgattcagg aagcttccgg tcatcaagcc ttcaataacc cattgcggat
    47101 cagcatcttt taatacaatt gccgggtttt taccgcccag ttccagcgtt acacgcgtta
    47161 agtgatcagc agcagttctg gcaatacctt ttcccgtcgc ggttgaaccg gtaaaactga
    47221 ttttcgcaac atgaggatgt gacgtcaggg ccgcgccgca tacagcacct gacccggtga
    47281 cgacattaaa aacgccatca gggataccag cctcgctggc cagttccgcc acgcgcaaca
    47341 tcgtcagtgg cgtggtttcc gaaggcttaa tcacgattga acagcctgct gccagtgctg
    47401 gcatcacctt ccacatacca atcatcaacg gaaagttcca tggcacaatt cccgccacta
    47461 cgccaaccgg ctctttacgc gtccaggcct gataacgcgc cccctggggt aagggaatcg
    47521 acaagtccag cgttttaccc gcgattttgg tcgttaaccc ggcggtataa cgcatccagt
    47581 tcagcgtaca gcccacttca aaagcacgag aaatggcaat tgacttgcct tgctccaggg
    47641 tttccagttg cgccagctcc tcactgtgct gctccaccag atcagcaaaa cgtagcagaa
    47701 tacgttcacg ctctgcgggt aatcgcccgg cccagcgacg cgagacaaag gcccgccagg
    47761 cagacatgac tgcgttatct acatccgctt cgttggcatc agcagtagac gcaatttctt
    47821 gcccggtggc cggatcaaag atcgccaatc gtttttcact ttgtgcgggg cctggacgac
    47881 catcaatata aagaccgtgt tgacgatcga gaaactgttg gacctggctt aatactgcta
    47941 catgcggctc tgtcatcact tttccttatt atttacccag tgtgatttca gtgtacgcag
    48001 cgacaagaaa agtcactttt ttcgctgtga cattcgcttt gtcagctgtg acaagctccg
    48061 caaatcgtga caataaccgc ttatgtctct atgcctgaga agacttattt cctatagtca
    48121 ggctcattag cttcgtttac tgcaacacaa atgcaacaat aaaaatacat ttcacagagc
    48181 gaaaacgtgc catgaacccc gcagtggata atgagtttca gcaatggctt tcccaaatca
    48241 atcaggtatg cggaaatttt accggacacc tgctgactga gcgttacacg ggtgtactgg
    48301 atacgcattt tgccaaagga ctaaagctga gtaccgtgac aaccagcggg gtgaatttat
    48361 cccgcacctg gcaggaagta aaaggcagcg acgatgcctg gttttacacc gtttttcagc
    48421 ttagtggtca ggcaataatg gagcaggatg agcgtcaggt gcagattggc gctggcgata
    48481 ttacgttact cgatgcctca cgcccctgtt cgctttactg gcaggagtct tctaaacaga
    48541 tttcattact tttgccacgc actctgctgg aacaatattt tccccatcaa aaacctgtct
    48601 gcgcagaaag actggacgct gacttaccca tggtgcaact cagtcatcgc ctgttacagg
    48661 agagcatgaa taatccggca ctttctgaaa cagaaagtga agctgcgcta caggcgatgg
    48721 tgtgtctgct gcgcccggta cttcatcagc gggaatctgt tcaacctcgt cgtgaacgtc
    48781 agtttcaaaa agtggttacg ttgatagacg ataatattcg cgaagagata ttacgcccgg
    48841 agtggatagc cggagagaca ggtatgtcag tacgtagttt gtaccgaatg tttgccgata
    48901 aaggtttggt agtcgcgcaa tatattcgta accgtcgtct cgatttttgt gcagatgcga
    48961 ttcgccatgc cgcagatgat gaaaaactgg caggcatcgg ctttcattgg ggattttctg
    49021 accagagtca tttttcaacg gtatttaagc aacgctttgg gatgacgcca ggcgagtatc
    49081 gacgtaaatt ccgctaacta attggcgttg tgtgtactgc aacgccaatt agttaaaaca
    49141 gatatttgac tatgcatttt tcagggcgaa aggcaatttg acaggagttt ttgaatattc
    49201 accttgtttc aggaaaatag atcaatcgct ttctcctgta gcgttatccc gatattgcgg
    49261 ttttgctatt aaaattttcg cacccattta ccgtttatcc cttgtacata ttcccccggt
    49321 ttggcccgag ctacgagctt ttgcccggcg agtttcgcga tatcatctac agacacatta
    49381 ttttgctttg ccagttgttg gtaactcgct ttacgttcgg cattaatgtc ggttactaat
    49441 ttctctgttt cagcatccgt tttcagcgca accagataac cataaaatgt ttcacctacc
    49501 cgcccctgag ttctggcttc atccagagtc aatgccatta cattgctgct taccagccca
    49561 acaagaaacg cacaaaaaag taatgttttc ttcatcatcg cctcagaaaa gatcgctacg
    49621 agtttcaagc agctcttcga catctttgtc tgccttgatg atgatctcat gctcaatttt
    49681 aacgttcata ttgatagtga tcggttcctt gggcgcagcg acttcaatac gcggcgtaca
    49741 ggcagccagc ataaaagatg acgtcaacgc agccagtaaa attttcattg tttttcctca
    49801 cactcttttc cttgcggaca gtcatttccc ggcagacgtg cgttctgctc caaccatgcc
    49861 tggagattat cgccaaagcg taaactgcgc cacagcgtaa aaatattctc ttcatgatgg
    49921 taattaaggt ttaccgtacc gcttttacca tcaacgcgac tggtacctgt aatgttggcc
    49981 tgcatggtta ataaaccgag attatctaag ttaatttttg tcgatgaccg gctaatttcc
    50041 atatagcgca accagttaat tgctgaaccc gcagtcatat tgtctttcac caccgcatct
    50101 gcggtgtctt tatccagccg caaggtcatt ggcccgctat ttgccagcca accatctttc
    50161 actatccatt tttcgctgtt tagccataac ggcaatgcac cactaaatgc cccggacatg
    50221 gcgaattgtt tcggattgac ggcgctaacc agttcgctgg atgaaagatt attcagccgc
    50281 aacagcgccg ggtcatgttg cggcatacgt aattgtttca tcagtacgtt accgcctaac
    50341 acatcgacgc taacatcagt caacagcaag ggttcttctt cagtccacgg ataacgccct
    50401 tgcaaatcag ccgtaatatt tttcgctgtc accagattaa tcacttcggc aattcgcaac
    50461 gtaacggggc cgcgagtccc cagatgccag gctccatcgg caaaacggaa aggcaggaca
    50521 aaatcgacac cgttaacctg attatctggc atccaggcac taccgccttt caacacgccg
    50581 tgtcctcccg cgcggaatcc ttgttcaggc gcagcagaaa atgcaacctg agcatacagt
    50641 tcaccatcgc gtaagttcat cttccagtcg ggtggcacca gcggctggaa tacagtcagt
    50701 gattgttttg gccaccaggc gttaccgcgc agacgaatac cgtcccagcg accatttacc
    50761 cgaaccgggc caatctcacc agcatgtaaa tcgcctttaa agaggaaata cgtcggatcg
    50821 cgcccatcga cgctaaattt taaggttgat ggcggtaaca cactgccgcc agtgaacagc
    50881 gtttgcccgg cgtccagtga cagcgcgccg ctgaaggagg gatgctgtgc gtcacgtacc
    50941 cagacgacgg gcttgtcgag aattaatcgc ggcttttcta ccgtcatcgt accgtattgt
    51001 aactggtcga aaccggtgga gaggtcggtc agcgtaatcg tgctgtcatg ccattcacct
    51061 tttcctgcga catcccaggt ggcattcatc ggtgtaaaac tgccttttcc ccagtagcgc
    51121 cactgccagc ggcccgcgtc agggagaaaa tcattcgcca gcccatccat atgcagcacg
    51181 aaatcgccca gttcattttc atgcgcctgc aaaatggcct gcaaacgtcc gtcaacacca
    51241 cgttgggtga cttttacacc cgctaaaggc cagcggattt catcgatgtc cagcgaatcg
    51301 atgactcttc ccttcgaacg taataacgcg cctggctcaa aggccagcgt tgggtcagtc
    51361 agacttccac ttagctgcgc gggtaagcgg gcatataaaa tgaggtccgc ctgtttcgct
    51421 tcaccggtca gttgcagagg cagctggctg ttatccatgc ttaattttcc tgggccaaaa
    51481 tttagcaccg cgttgccttt acccgcttgc ccctgggtca gcacactcag tcgtccgctg
    51541 atcagagcgt tctcaagccc tgcctgccag ttgtcgactt tgacacccag tcggccactc
    51601 aaaggaaaac ctgcatacgg ccaactccag cgaccatcgc ttacggtcaa ttgttgacga
    51661 gtaatttgcc acggcaaatc gagcaacgga tcgccgttat cccgtgccag cacaatcaat
    51721 tgcccgctat tttcctgcca gtccagctct gcatccacca gtgacggttc ctgcggcaag
    51781 tttaacgtcg cagtagcatg cccacttaca ggaagtccat ccggcacgag cggcatagta
    51841 aattccccca ccagtttaac cggcggctga ttttcaaacg cgacgacatc cagttcgctg
    51901 actgtaagtt gttgcccttt cagctggcct tgaaatttaa ctttttcgcc ctgataacgc
    51961 agttgctgga tatcagaggt taatgcgaga gagagttttc cctgccattc ctgccaggga
    52021 gaaaaaatca gtttatcgat attgatccag gtgttaggca gcatggactg ccactgcgcg
    52081 agggtttttg gagcagctgg cgattgctcc gtctgcggca atttcgccag acaagcagaa
    52141 tcaagttcta ccgtgccgac gttcaataac cagcggctgg gatgtgaaag gctggcgttg
    52201 gtgatatgcg caagctgaca atctcccacc agataacgga gatcgggaat gattaaacct
    52261 ttacgcgtaa tgcgtgggct ttcatctaat gcaatacgtg tcccgagcgg tagccagatg
    52321 cccgccagag tgggaaccca cagcccgagc gtcatcaaca gcgtcaacgg cacaagaata
    52381 atcagtaata acagcgcgag aacggcttta tatttaccca gcatgggtag ttaatatcct
    52441 gatttagcga aaaattaagc attcaatacg ggtattgtgg catgtttaac cgttcagttg
    52501 aaggttgcgc ctacactaag catagttgtt gatgaatttt tcaa
//
LOCUS       DAZGOB010000019        50193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGOB010000019 DAZGOB010000000
VERSION     DAZGOB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.019"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHLIF_001160"
     CDS             complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHLIF_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO9605213.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHLIF_001161"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHLIF_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9605214.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHLIF_001162"
     CDS             complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHLIF_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO9605215.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHLIF_001163"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHLIF_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO9605216.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="ACHLIF_001164"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="ACHLIF_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO9605217.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="ACHLIF_001165"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="ACHLIF_001165"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9605218.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHLIF_001166"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHLIF_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO9605219.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="ACHLIF_001167"
     CDS             complement(7218..7283)
                     /locus_tag="ACHLIF_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605220.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHLIF_001168"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHLIF_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO9605221.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8704..9207
                     /gene="dps"
                     /locus_tag="ACHLIF_001169"
                     /gene_synonym="pexB"
     CDS             8704..9207
                     /gene="dps"
                     /locus_tag="ACHLIF_001169"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO9605222.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9611..10357
                     /gene="glnH"
                     /locus_tag="ACHLIF_001170"
     CDS             9611..10357
                     /gene="glnH"
                     /locus_tag="ACHLIF_001170"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO9605223.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10496..11155
                     /gene="glnP"
                     /locus_tag="ACHLIF_001171"
     CDS             10496..11155
                     /gene="glnP"
                     /locus_tag="ACHLIF_001171"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO9605224.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVLYLIITLVLSFILRRLERRMKI
                     L"
     gene            11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHLIF_001172"
     CDS             11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHLIF_001172"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO9605225.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHLIF_001173"
     CDS             11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHLIF_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO9605226.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHLIF_001174"
     CDS             complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHLIF_001174"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO9605227.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            15415..15660
                     /gene="mcbA"
                     /locus_tag="ACHLIF_001175"
                     /pseudo
     CDS             15415..15660
                     /gene="mcbA"
                     /locus_tag="ACHLIF_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
     gene            15938..18220
                     /gene="fiu"
                     /locus_tag="ACHLIF_001176"
     CDS             15938..18220
                     /gene="fiu"
                     /locus_tag="ACHLIF_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO9605228.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            18262..18939
                     /gene="ybiX"
                     /locus_tag="ACHLIF_001177"
     CDS             18262..18939
                     /gene="ybiX"
                     /locus_tag="ACHLIF_001177"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO9605229.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            19013..19279
                     /gene="ybiI"
                     /locus_tag="ACHLIF_001178"
     CDS             19013..19279
                     /gene="ybiI"
                     /locus_tag="ACHLIF_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO9605230.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKGLQKPAYTGYSRRGSKDSQLR"
     gene            19544..19804
                     /gene="ybiJ"
                     /locus_tag="ACHLIF_001179"
     CDS             19544..19804
                     /gene="ybiJ"
                     /locus_tag="ACHLIF_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO9605231.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(20036..20518)
                     /gene="ybiA"
                     /locus_tag="ACHLIF_001180"
     CDS             complement(20036..20518)
                     /gene="ybiA"
                     /locus_tag="ACHLIF_001180"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO9605232.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            20638..22788
                     /gene="dinG"
                     /locus_tag="ACHLIF_001181"
     CDS             20638..22788
                     /gene="dinG"
                     /locus_tag="ACHLIF_001181"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO9605233.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            22816..23778
                     /gene="ybiB"
                     /locus_tag="ACHLIF_001182"
     CDS             22816..23778
                     /gene="ybiB"
                     /locus_tag="ACHLIF_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO9605234.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            23919..25004
                     /gene="hcxB"
                     /locus_tag="ACHLIF_001183"
     CDS             23919..25004
                     /gene="hcxB"
                     /locus_tag="ACHLIF_001183"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO9605235.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHKHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            25071..25130
                     /gene="ybiE"
                     /locus_tag="ACHLIF_001184"
     CDS             25071..25130
                     /gene="ybiE"
                     /locus_tag="ACHLIF_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO9605236.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(25235..26599)
                     /gene="rhlE"
                     /locus_tag="ACHLIF_001185"
     CDS             complement(25235..26599)
                     /gene="rhlE"
                     /locus_tag="ACHLIF_001185"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO9605237.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGDGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            26828..27499
                     /gene="cecR"
                     /locus_tag="ACHLIF_001186"
     CDS             26828..27499
                     /gene="cecR"
                     /locus_tag="ACHLIF_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO9605238.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            27502..28497
                     /gene="hlyD"
                     /locus_tag="ACHLIF_001187"
     CDS             27502..28497
                     /gene="hlyD"
                     /locus_tag="ACHLIF_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO9605239.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            28490..30226
                     /gene="ybhF"
                     /locus_tag="ACHLIF_001188"
     CDS             28490..30226
                     /gene="ybhF"
                     /locus_tag="ACHLIF_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9605240.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            30219..31352
                     /gene="ybhS"
                     /locus_tag="ACHLIF_001189"
     CDS             30219..31352
                     /gene="ybhS"
                     /locus_tag="ACHLIF_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9605241.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            31363..32469
                     /gene="ybhR"
                     /locus_tag="ACHLIF_001190"
     CDS             31363..32469
                     /gene="ybhR"
                     /locus_tag="ACHLIF_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9605242.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(32431..32841)
                     /gene="ybhQ"
                     /locus_tag="ACHLIF_001191"
     CDS             complement(32431..32841)
                     /gene="ybhQ"
                     /locus_tag="ACHLIF_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO9605243.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            32974..33735
                     /gene="ybhP"
                     /locus_tag="ACHLIF_001192"
     CDS             32974..33735
                     /gene="ybhP"
                     /locus_tag="ACHLIF_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO9605244.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPKGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            33732..34973
                     /gene="clsB"
                     /locus_tag="ACHLIF_001193"
     CDS             33732..34973
                     /gene="clsB"
                     /locus_tag="ACHLIF_001193"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO9605245.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            34973..35929
                     /gene="ybhN"
                     /locus_tag="ACHLIF_001194"
     CDS             34973..35929
                     /gene="ybhN"
                     /locus_tag="ACHLIF_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO9605246.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPVGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(35965..36678)
                     /gene="ybhM"
                     /locus_tag="ACHLIF_001195"
     CDS             complement(35965..36678)
                     /gene="ybhM"
                     /locus_tag="ACHLIF_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO9605247.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(36883..37587)
                     /gene="ybhL"
                     /locus_tag="ACHLIF_001196"
     CDS             complement(36883..37587)
                     /gene="ybhL"
                     /locus_tag="ACHLIF_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO9605248.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(37724..38176)
                     /gene="moaE"
                     /locus_tag="ACHLIF_001197"
     CDS             complement(37724..38176)
                     /gene="moaE"
                     /locus_tag="ACHLIF_001197"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO9605249.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(38178..38423)
                     /gene="moaD"
                     /locus_tag="ACHLIF_001198"
     CDS             complement(38178..38423)
                     /gene="moaD"
                     /locus_tag="ACHLIF_001198"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO9605250.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(38416..38901)
                     /gene="moaC"
                     /locus_tag="ACHLIF_001199"
     CDS             complement(38416..38901)
                     /gene="moaC"
                     /locus_tag="ACHLIF_001199"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO9605251.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVTALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(38904..39416)
                     /gene="moaB"
                     /locus_tag="ACHLIF_001200"
     CDS             complement(38904..39416)
                     /gene="moaB"
                     /locus_tag="ACHLIF_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO9605252.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(39438..40427)
                     /gene="moaA"
                     /locus_tag="ACHLIF_001201"
     CDS             complement(39438..40427)
                     /gene="moaA"
                     /locus_tag="ACHLIF_001201"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO9605253.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(40415..40559)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            40824..41732
                     /gene="yvcK"
                     /locus_tag="ACHLIF_001202"
     CDS             40824..41732
                     /gene="yvcK"
                     /locus_tag="ACHLIF_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO9605254.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(41924..43945)
                     /gene="uvrB"
                     /locus_tag="ACHLIF_001203"
     CDS             complement(41924..43945)
                     /gene="uvrB"
                     /locus_tag="ACHLIF_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO9605255.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(44524..45201)
                     /gene="bioD"
                     /locus_tag="ACHLIF_001204"
     CDS             complement(44524..45201)
                     /gene="bioD"
                     /locus_tag="ACHLIF_001204"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO9605256.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(45194..45949)
                     /gene="bioC"
                     /locus_tag="ACHLIF_001205"
     CDS             complement(45194..45949)
                     /gene="bioC"
                     /locus_tag="ACHLIF_001205"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO9605257.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(45936..47090)
                     /gene="bioF"
                     /locus_tag="ACHLIF_001206"
     CDS             complement(45936..47090)
                     /gene="bioF"
                     /locus_tag="ACHLIF_001206"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO9605258.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(47087..48127)
                     /gene="bioB"
                     /locus_tag="ACHLIF_001207"
     CDS             complement(47087..48127)
                     /gene="bioB"
                     /locus_tag="ACHLIF_001207"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO9605259.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            48214..49503
                     /gene="bioA"
                     /locus_tag="ACHLIF_001208"
     CDS             48214..49503
                     /gene="bioA"
                     /locus_tag="ACHLIF_001208"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO9605260.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            49562..50038
                     /gene="ybhB"
                     /locus_tag="ACHLIF_001209"
     CDS             49562..50038
                     /gene="ybhB"
                     /locus_tag="ACHLIF_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO9605261.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT        11949 a        13261 c        12953 g        12030 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttctg catctgatgg gctcgcatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcaatcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag cgaaggaaat
     6481 aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaaacctta
     6601 aaacaaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtacctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattccgt ggtctggaat ttaccataac
     6841 ccacccccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agagccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccatcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta catttctgca tggttatgca taaccatgca gaatttctcg ctacttttcc
     8461 tctacaccgt ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc
     8521 gagcagataa ataagaattg ttcttatcaa tatatctaac tcattgaatc tttattagtt
     8581 ttgtttttca cgcttgttac cactattagt gtgataggaa cagccagaat agcggaacac
     8641 atagccggtg ctatacttag tctcgttaat tactgggaca taacatcaag aggatatgaa
     8701 attatgagta ccgctaaatt agttaaatca aaagcgacca atctgcttta tacccgcaac
     8761 gatgtctccg acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca ggttatccag
     8821 tttattgatc tttctttgat taccaaacaa gcgcactgga acatgcgcgg cgctaacttc
     8881 attgccgtac atgaaatgct ggatggcttc cgcaccgcac tgatcgatca tctggatacc
     8941 atggcagaac gtgcagtgca gctgggcggt gtagctctgg ggaccactca agttatcaac
     9001 agcaaaaccc cgctgaaaag ttacccgctg gacatccaca acgttcagga tcacctgaaa
     9061 gaactggctg accgttacgc aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg
     9121 aaagatgacg acaccgcaga tatcctgacc gccgcgtctc gcgacctgga taaattcctg
     9181 tggtttatcg agtctaacat cgaataaatc catcgctgat ggtgcagaac tttagtaccc
     9241 gataaaagcg gcttcctgac aggaggccgt tttgttttgc agcccacctc aacgcactta
     9301 tttagtgcat ccatctgcta tctccagctg attaagtaaa ttttttgtat ccacatcatc
     9361 acacaatcgt tacataaaga ttgttttttc atcaggtttt acgctaaata atcactgtgt
     9421 tgagtgcaca attttagcgc accagattgg tgccccagaa tggtgcatct tcagggtatt
     9481 gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaac
     9541 tggcacgatt ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa
     9601 aggaaatgct atgaagtctg tattaaaagt ttcactggct gcactgaccc tggcttttgc
     9661 ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg acggataccg ccttcgttcc
     9721 gtttgaattt aaacagggcg ataaatatgt gggctttgac gttgatctgt gggctgccat
     9781 cgctaaagag ctgaagctgg attacgaact gaagccgatg gatttcagtg ggatcattcc
     9841 ggcactgcaa accaaaaacg tcgatctggc gctggcgggc attaccatca ccgacgagcg
     9901 taaaaaagcg atcgatttct ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa
     9961 agctaacaat aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa
    10021 gagcggtact ggctccgttg attacgcgaa agcaaacatc aaaactaaag atctgcgtca
    10081 gttcccgaac atcgataacg cctatatgga actgggcacc aaccgcgcag acgccgttct
    10141 gcacgatacg ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc
    10201 ggtaggtgac tctctggaag cgcagcaata cggtattgcg ttcccgaaag gtagcgacga
    10261 gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga
    10321 aatctacaaa aaatggttcg gtactgaacc gaaataataa cgctacacct gtaaaacgca
    10381 ctggcagttc cctctcccct ctggggagag gattagggtg aggggcgcaa acccgctccg
    10441 gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac aacatatgca
    10501 gtttgactgg agtgccatct ggcctgccat tccgcttctg attgaaggtg ccaaaatgac
    10561 cctgtggatt tcggtcctcg gtctggcagg cggtctggta atcggattgc tggcaggttt
    10621 tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat
    10681 ccgcggcaca cctatcgtcg tccaggtgat gtttatctat ttcgccctgc cgatggcgtt
    10741 taacgactta cgcatcgacc catttactgc ggcggtggtc accatcatga tcaactccgg
    10801 cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga
    10861 agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca ttttaccgct
    10921 ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca ttaaagacac
    10981 ctcgctgttt attgtgatcg gcgtggcgga actgacccgt caggggcaag aaattattgc
    11041 cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgctctatc tgattattac
    11101 cctggtgctg agctttattc tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa
    11161 tttaaaaacg tctccaagca ctttggccca acccaggtgc tgcataatat cgatttgaac
    11221 attgcccagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa atcgaccctg
    11281 ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt cgatggcctg
    11341 aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc
    11401 cagcagtttt acctcttccc gcatctgacg gcgctggaaa acgtcatgtt tggtccgcta
    11461 cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa
    11521 gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg tcaacagcag
    11581 cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg
    11641 acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca ggatctggct
    11701 gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga gaaagtagct
    11761 tcgcggctga tctttatcga caaaggccgg attgcggaag atggcgatcc gcaggtattg
    11821 atcaagaacc cgccgagcca gcgcttgcag gaatttttgc agcacgtctc ttaacttctc
    11881 tgcctccctt tcacccgaaa gggaggcaca ccagattcct ctcatttaaa atcgcccctc
    11941 ccccagcatc tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga
    12001 tcctgttcat cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg
    12061 ttacaaccac aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac
    12121 aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag
    12181 aattgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga
    12241 tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc
    12301 gccattatgg tgaagccctt tccgcccgct tcgggcaact ttatcgcaat atcaccggct
    12361 ccccgcataa gccatttaat ccacaaacct tcagcaatgc gctgagccat ttttcaatgt
    12421 tagcggtatt agtgtttggt ttttactggc tgattcgcct gtgcgcactg ccgctgtatc
    12481 gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc
    12541 cggcgatgat tatcggggcg tttattatcg acctgctgtt actggccctg acattatttg
    12601 tcggccaggt attaagcgac aacctgaatg caggcagtcg caccatcgct ttccaacaaa
    12661 gtttgtttct caatgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt
    12721 tttgcccaaa cgtggcggag ctgcgtccgt tcacgattca ggacgagagc gcccgttact
    12781 ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta tggcctgatt gtggccgtgc
    12841 cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc
    12901 tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa taaaaaagag attacccagc
    12961 atttgctcaa ctttgcggag cattcgctgg cctttttcag cctgtttatc cgcgcctttg
    13021 cgctggtgtg gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt
    13081 tcgatccggg caacagcctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta
    13141 ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg gctggcgaaa accatcaccc
    13201 tctcgccaca tactcagcgt aactatccgg agctacaaaa acggctgaat ggctggcttt
    13261 cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcagtaatg ttgctgttga
    13321 gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg
    13381 tagatatcct gatccgcatc gcactcattc ttttcttctc ggcggttggc tggacggtgc
    13441 tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg
    13501 cccgtacgcg taccctgctg acgctgtttc gtaacgcgct ggcggtgatt atcagtacca
    13561 tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg
    13621 ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtgaaa gatatcatta
    13681 ccggggtgtt tattcagttt gaaaacggca tgaacactgg agatttggtg actatcgggc
    13741 cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc caggataccg
    13801 gggcgtatca catcattccg tggtcttcga taaccacctt tgctaacttc gtccgcggta
    13861 ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc
    13921 aggcactgaa agatgcggta gcggaattga tggaaaacga agaaattcgc gggctgatta
    13981 ttggtgaacc gaattttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg
    14041 tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc gacagccagg
    14101 tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt gcagacttat caggtgctgc
    14161 ctgctccggg cgcgaccccg gctgaaccgc cgccgccggg ggagccaacg ctttaacgtt
    14221 ggcgattaac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac
    14281 tctgcttttg cccctgggcc atctcttttt ttaccacctt caccgcgccc acgtcggtca
    14341 gagcacgata caacggcggt aagttttcac cacgagatac cagcgaggta aaccacatca
    14401 cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaaggcg acttcaccac
    14461 cttcacacca caactcctgt tgctgaccac caaagttcag tgcatcgtct ttgttcagcc
    14521 ccaggttacg acgtttacgc tcactacctg cccgcgctgc agcggcggaa tcgtggaatg
    14581 gcgggttaca caaggtcgcg tcgtattgct cgtttttatg gatgatgccg ttaaaaattg
    14641 ccccactctc tttttgccga cgcagacgaa tggcgcggtt aagccccgga ttagcactga
    14701 taatcgcctg cgcactgctt aacgcctggc tgctggtttc actaccggta aaacgccagc
    14761 catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg atatccagaa
    14821 tgctggcatt cgctggaatt gttccgcttg cttcagccag taaatcggca aggtgatgaa
    14881 tataatccgc ccggcccggt actggtgggc agagaaaacc gtcggggata tcccagttcg
    14941 ctacggcgta aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc agcggattgg
    15001 caaagtctac gctttgctcc ccggcgggtg taagcgtgag gaattgcctg agttcaggat
    15061 tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc
    15121 ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac
    15181 gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt
    15241 ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttgatttgtc ttattttgta
    15301 aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca ttttcatcgc
    15361 ccaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa
    15421 aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc
    15481 gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca
    15541 actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgagcaa
    15601 ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt cggtactgca
    15661 ccatctacaa ataaccccac caacgtaact gcattcgcct ctggatgcag ttttcgcctg
    15721 ttttgtaatc aaaaaacatc aaatatgaac gcaatgtaaa taaatgtatt tctttttcgc
    15781 gcaatgggtg atagaaaatc gcgccaagtg ataatgctta tcaaaattat tatcacttgc
    15841 acgaacacta tcacgagatt aacagtggca tcgcatccgc agagaggctt tctcgtggca
    15901 gtgaaaattt caacatataa aaaaagtcac ctgcaaaatg gaaaacaatc gcaatttccc
    15961 tgccagacaa tttcattcgc tcacgttctt tgccggtctt tgtattggca tcacgcctgt
    16021 ggctcaggca ctcgccgccg aagggcaaac taacgcggat gacacgctgg ttgtcgaagc
    16081 atcaacgcct tcgctttatg cgccacaaca atctgccgat ccgaaattct cgcgtccggt
    16141 agcggatact acccgcacga tgacggtgat ttctgaacaa gtgattaaag atcagggcgc
    16201 aaccaacctt accgacgcgc tcaaaaacgt ccccggcgtg ggtgcgtttt ttgcgggtga
    16261 gaacggtaac tccaccactg gcgacgccat ttatatgcgc ggtgctgata cctctaacag
    16321 tatttatatt gatggcattc gcgatatcgg cagcgtctcg cgcgacacct tcaatactga
    16381 gcaggtcgaa gtgattaaag ggccttccgg caccgactac gggcgcagcg cgccgacggg
    16441 ctcgatcaat atgatcagca agcagccgcg caatgattcc ggcattgacg cctccgccag
    16501 tattggcagc gcctggttcc gccgcggcac gctggacgtc aatcaggtca ttggtgatac
    16561 cactgcggtg cgcctgaatg tgatgggcga aaaaacgcac gatgccggac gcgacaaagt
    16621 caaaaatgag cgttacggcg tcgccccttc tatcgctttt ggccttggta cagcgaatcg
    16681 tttgtatctt aattatctgc atgtcaccca gcacaacacg ccagacggcg gcattccgac
    16741 catcggtttg ccgggttatt ctgccccatc tgcgggaaca gcggctctga atcattccgg
    16801 aaaagttgat actcataact tttacggcac ggattccgat tacgacgatt cgaccaccga
    16861 caccgccacc atgcgttttg agcacgacat caacgataac accaccattc gcaatactac
    16921 ccgttggtcg cgcgtaaagc aggattacct gatgacggcg attatgggcg gggcgtcgaa
    16981 tattactcag cccaccagcg atgtgaatag ctggacctgg tcacgcacgg cgaataccaa
    17041 agatgtgagt aataaaattc tcaccaacca gaccaacctg acctcgacgt tctataccgg
    17101 ttctatcggt catgatgtca gtaccggcgt ggaatttacc cgtgaaacgc agactaacta
    17161 cggcgttaat ccggtgacgt tacccgcggt aaatatttat catcctgaca gcagcattca
    17221 tcccggcggc ctgacgcgca acggcgcaaa cgccaatggt cagacggata ccttcgcaat
    17281 ttacgccttc gatacgctgc aaatcacccg tgattttgag ctgaacggcg ggatccgtct
    17341 ggataattat catactgaat atgacagtgc caccgcctgc ggcggcagcg gacgcggtgc
    17401 catcacctgc ccaactggtg tggcaaaagg ttctccggtc accaccgtcg acaccgccaa
    17461 gtcgggcaat ctggtgaact ggaaagccgg ggcgctgtat cacctgacgg aaaacggtaa
    17521 tgtctatatt aactatgccg tttcccagca gcctccgggc ggcaacaact tcgcccttgc
    17581 gcagtctggc agcggtaaca gtgccaaccg caccgatttt aaaccacaaa aagccaacac
    17641 cagcgagatt ggcaccaaat ggcaggttct ggataaacgc ctgttgctca ccgccgcgct
    17701 attccgtact gatatcgaaa atgaagttga gcaaaatgat gacggaactt actcgcaata
    17761 cggtaagaaa cgtgtcgaag gctatgagat atccgtggcc gggaatatca cgcccgcgtg
    17821 gcaggtgatt ggcggctata cccagcaaaa agcaaccatc aaaaacggca aagatgttgc
    17881 ccaggatggt tcctcatcgc tgccgtatac cccggagcac gccttcacct tatggagcca
    17941 gtatcaggca acagatgata tctctgttgg cgcgggcgca cgctatatcg gcagtatgca
    18001 taaaggttca gacggcgcgg tgggaacgcc agcgtttacc gaaggttact gggtcgccga
    18061 tgccaaactg gggtatcggg ttaatcgcaa tctcgacttc cagctaaacg tttacaacct
    18121 gtttgatacc gattacgtcg cctcaatcaa caagagcggc taccgttatc acccgggcga
    18181 gccaagaacc ttcttgctca cagccaatat gcatttctga ttcagatgtg gggcgcaggc
    18241 cccacttttt ggagaacttg tatgatgtac cacattcccg gcgtgttatc gccacaggac
    18301 gtcgcccggt ttcgcgaaca actggaacaa gccgaatggg tggatggacg cgtcaccacc
    18361 ggcgcacaag gtgcgcaagt taagaacaat caacaggtcg acacccgcag cacgttatac
    18421 gccgccctgc aaaatgaggt gctgaacgcg gttaaccaac atgctttatt ctttgccgcg
    18481 gccttgccgc gtaccctttc cacgccgctg tttaatcgct atcagaacaa tgaaacctat
    18541 ggtttccatg tggatggcgc agtacgcagc catccacaaa acggctggat gcgtactgac
    18601 ctttctgcca cgctgttttt aagcgatcca caaagctacg acggcggcga actggtcgtt
    18661 aatgacacct tcggacaaca tcgggtaaaa ctcccggcag gcgatctcgt gttgtatccc
    18721 tccagcagcc tgcattgcgt gacacccgta acccgcggcg tacgagtggc atcatttatg
    18781 tggatccagt cgatgatccg tgatgataaa aagcgcgcca tgctgtttga actggacaac
    18841 aatattcagt cgctgaaaag ccgctacggc gaaagtgaag agatcctgtc gctgcttaat
    18901 ctttatcata atctgctgcg ggaatggtcg gagatctgat gcttgaactg cccaggttcg
    18961 tccctggatt agctacactt aactgtacaa gcattgatat ggggaggacg atatggcatc
    19021 cggttgggct aacgatgacg ccgtcaacga acagatcaac agtacaattg aagatgcgat
    19081 tgcccgcgct cggggtgaaa ttccgcgtgg cgaaagcctg gatgaatgtg aagagtgcgg
    19141 tgcccccatc ccgcaagccc gtcgggaagc cattcctggc gtgcgcttat gcattcattg
    19201 tcagcaggag aaaggtttac aaaaaccagc gtatacagga tatagtcgca gaggttcgaa
    19261 agacagccag ttacgttaac tacaactggc gagatgcata gcgagtataa tttgtatttt
    19321 gcgtggcatt ccgtgccttt aacgctgctg tgactgccac gcggcataaa acaagtccat
    19381 aagtacaaca aataaatggt ttatcagtat gttagatatc aatcaattta tttgaacaag
    19441 gcggtcaatt ctcttcgatt ttatctctcg taaaaaaacg tgatactcat cacatcgacg
    19501 aaacaacgtc acttatacaa aaatcacctg cgagagatta attatgaaaa ctatcaatac
    19561 tgttgttgct gctatggctc tttcaactct gtcatttggc gtgtttgccg cggaaccggt
    19621 aacggcatcc caggcacaga acatgaataa aatcggcgtg gtttctgccg atggcgcatc
    19681 caccctcgat gccctggaag cgaaactggc tgagaaagcc gcggcagccg gtgccagtgg
    19741 atacagcatc acttccgcca ccaacaacaa taaattaagc ggtactgcgg taatttataa
    19801 gtaattcgta ggacggataa ggcgtttacg ccgcatccgg caacagatgc ctgatgcgac
    19861 gctaccgcgt cttatcagta tctacataat ctctgcaatc tattgaattt gcatgttttt
    19921 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    19981 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    20041 tctctatagc caattgttcg cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    20101 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    20161 gcgcggttgc cagcaagagc gcacgcagtt ctggatgctg ttcgaattta gcgcgaagtg
    20221 cttttcgcat cacctgttct ttgaccgact cccagttttt acgcagaggc ttagaacgat
    20281 cgcgccccat gcgtgcggcg accatagggg aagaaacccg acggatctct tcgcggtatt
    20341 tttcatcaag gaatttttgt gcctgaaaat agtgttctga ggtgggccag gttttcccgt
    20401 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    20461 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    20521 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    20581 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    20641 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    20701 atccccgact ttattccccg tgcgccgcag cggcagatga ttgcggacgt cgccaaaacg
    20761 ctggccggag aagaagggcg acatctggcg attgaagccc ccaccggcgt tgggaaaacg
    20821 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    20881 agtaccgcca acgtggcatt gcaggatcag atttacagca aagatttacc gctgctgaaa
    20941 aagatcattc ccgatcttaa attcactgcc gcttttgggc gtgggcgcta cgtttgcccg
    21001 cgtaatctga cggcgctcgc cagcactgag cccacgcaac aggatctgct ggcgtttctt
    21061 gacgacgaac tgacgccaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    21121 gatctcgaca cttataaatg ggatggtctg cgcgatcata ctgatatcgc tatagatgac
    21181 gatctctggc gtcgtttaag taccgacaaa gccagctgcc tcaaccgcaa ctgttactac
    21241 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    21301 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactacc tgacccgaaa
    21361 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    21421 gagatgagtg ccgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    21481 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    21541 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    21601 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    21661 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    21721 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccacga tattgtgcgt
    21781 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    21841 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    21901 cgggaagagc gcgaagggca gctgcatctc tggtttcact gcgtgggaat acgtgtcagc
    21961 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    22021 ttgcgttcgc tgaacagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    22081 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    22141 attccccgga tgcgctttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    22201 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    22261 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg cctgatgctg
    22321 ctggtgcagg gcgatcagcc gcgttatcgc ttagttgaac tgcaccgcaa acgcgttgcc
    22381 aacggtgagc gtagtgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    22441 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccg
    22501 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    22561 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    22621 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    22681 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    22741 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    22801 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    22861 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgcccatat gctcaatggt
    22921 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggggaaggg
    22981 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    23041 cctgcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    23101 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    23161 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    23221 acgccaacgt tgcacggcgg acaggcgcag gcgaagctcg acgaacatca accggtgttt
    23281 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    23341 ggcgtgcgta acagtgcaca taccctggcg aaactggcaa cgccatttgc cgaaggtgag
    23401 gcgctgcgtc tttccagcgt ttcgcatccg gaatacattg gacgcgtcgc gaagttcttt
    23461 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    23521 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    23581 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    23641 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    23701 atggcttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    23761 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    23821 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    23881 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    23941 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    24001 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    24061 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    24121 accatcatgc caaaaccgtt aaagagtcgg gggcggcggt cacgctcgat ggcgatcgcg
    24181 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcataagc
    24241 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    24301 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    24361 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    24421 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    24481 ttggtaaaac tcgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    24541 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggggcgc
    24601 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    24661 gggcgctttc tggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    24721 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    24781 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccgcatgat gatgataagc
    24841 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    24901 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    24961 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    25021 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    25081 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    25141 ggttcggatt ttgtagatac tgataagcgt agcgcatcag gcagttttgc gtttgttcat
    25201 cagcctgatg ccgggcatag cccggcataa aagattactg cgcagcggta ggtttacgcg
    25261 gacggcggcg acgttgttgt tcgcctgccg gtttggcatc gccgaggcgg cgagacggtt
    25321 tttctgcagg tttcgcgctt gcagattttg cgccaccttc cccacggcgt ggttgctgtt
    25381 gaccgcgacc accaccttgc ccacgaccgc cgtcgccacg ttgctggcga ccgttctgga
    25441 tcggttcggc tttgattgac gggtccggct catagcccgg aatcgcaatg cgcgggatct
    25501 cttttttcag cagtttttcg atatcacgca gcagtttgtg ttcatcaaca cacaccagcg
    25561 acaacgcttc accggtagca gccgcacgac cggtacgccc gatacggtgg acataatctt
    25621 ccggtacgtt tggcagttca tagttgacga cgtgcggcaa ctcttcgata tccagaccgc
    25681 gcgcagcgat atcggtcgcc accagtacgc gaatgtcacc cgatttaaaa tcagccagcg
    25741 cacgggtacg cgcaccttgc gatttattcc cgtggatcgc cgcactacgg atgccatctt
    25801 tattgagctg ctcagccaga tggttagcgc cgtgtttggt acgggtaaac accagcacct
    25861 gctgccagtt ccctttacca atcatgtgcg acagcaattc gcgtttgcgt ttcttatcaa
    25921 caaagtgaac gtgctgggtc acctgatcag acgcggtatt gcggcgtgcc acttcgattt
    25981 ccagcgggtt gtgcaacagt ttttccgcca gggctttaat atcgtcagag aaggtcgcag
    26041 agaacagcag gttctggcgc ttcgctggca gttttgttaa cacgcgacgg atatcgtgga
    26101 taaagcccat gtcgagcatg cggtcagctt catcgaggac gaggatttca acctgatcca
    26161 gcttcactgc attttgatgt tccaggtcca gcaaacgtcc cggtgttgcc accagaacat
    26221 caacgccgcc gcgcagcttc atcatttgcg ggttaatgct gacaccgcca aacaccacca
    26281 gcgaacgaat gttcaaatat ttgctgtaat cacggacgtt ttcgccaatc tgcgcagcca
    26341 gttcacgggt cggggtaaga atgagcgcac gtaccggacg acgccctttg gcgtgcggct
    26401 ggcgagtgat caggtgttgc aacagcggca gcgtaaagcc cgctgtttta ccggtaccgg
    26461 tctgggcgct agccatcagg tcgcggcctt ccagcaccgc agggatcgcc tgctgctgaa
    26521 taggggtggg ttcacggtaa ccctgctcgg caacggcgcg caggatatca gggcttaaac
    26581 ccaaagaatc gaaagacata actactccga accgccccga ccgttaccgg tgtagttttc
    26641 agggagatac tgaaaaggaa aatgacaaaa accacaatgt catgagggag cggagtgtag
    26701 cagtttttgt gacgcagcgc ataaattatc cctgtgactg gcgtagctaa aattcttagc
    26761 catactggac aactcccata tggagtcata atcttaatca atcatttgat taagaatgga
    26821 ctgtgcgatg aataatcctg ccatgacaat caagggtgaa caggcgaaaa aacagctgat
    26881 tgctgccgca ctggcgcagt ttggtgaata tggaatgaac gccaccaccc gcgagatagc
    26941 cgcccaggcc gggcagaata tcgctgccat cacctactac ttcggttcga aagaagattt
    27001 gtacctcgcc tgcgcccagt ggattgccga ttttattggc gagcagttcc gtccgcacgc
    27061 cgaagaggcc gaacgcttgt tcgcacaacc acagcctgat cgcgcagcca tccgtgaact
    27121 gatccttcgc gcctgcagga acatgattaa gctgctcacc caggatgaca ccgtcaacct
    27181 cagcaagttt atctcccgtg agcagctctc tcccacggca gcctatcacc tggtgcacga
    27241 acaggtgatt agcccgctac acagccacct gacacgcctg attgccgcct ggaccggctg
    27301 cgacgccaac gatacccgca tgatcctcca tactcatgcg ttgattggtg agattctggc
    27361 gttccgtctt ggcaaagaaa caattctgtt acgtaccggc tggaccgcgt tcgatgaaga
    27421 aaagaccgaa ctgatcaacc agacggtgac ctgtcatatc gacctgattt tgcaaggatt
    27481 atcgcaaagg agtttgtagt gatgaaaaaa cctgtcgtga tcggactggc ggtagtggta
    27541 cttgccgccg tggttgccgg aggctactgg tggtatcaaa gccgccagga taacggcctg
    27601 acgctgtatg gcaacgtgga tattcgtacg gtgaatctta gtttccgtgt tggggggcgc
    27661 gttgaatcgc tggcggtgga cgaaggtgat gctatcaaag cgggccaggt gctgggcgaa
    27721 ctggatcaca agccgtatga gattgccctg atgcaggcga aagcgggtgt ttcggtggca
    27781 caggcgcagt atgacctgat gcttgccggg tatcgcgatg aagaaatcgc tcaggccgcc
    27841 gcagcggtga aacaggcgca agccgcctat gactatgcgc agaacttcta taaccgccag
    27901 caagggttgt ggaaaagccg caccatttcg gcaaacgacc tggaaaatgc ccgctcctcg
    27961 cgcgaccagg cgcaggcaac gctgaaatca gcacaggata aattgcgtca gtaccgttcc
    28021 ggtaaccgtg aacaggacat cgcccaggcg aaagccagcc tcgaacaggc gcaggcgcaa
    28081 ctggcgcagg cggagttgaa tttgcaggac tcaacgttga tagccccgtc tgatggcacg
    28141 ctgttaacgc gcgcggtgga gccaggcacg gtcctcaatg aaggtggcac ggtgtttacc
    28201 gtttcactaa cgcgtccggt gtgggtgcgc gcttatgttg atgaacggaa tcttgaccag
    28261 gcccagccgg ggcgcaaagt gctgctttat accgatggtc gcccggacaa gccgtatcac
    28321 gggcagattg gtttcgtttc gccgactgct gaatttaccc cgaaaaccgt cgaaacaccg
    28381 gatctgcgta ccgacctcgt ctatcgcctg cgtattgtgg tgaccgacgc cgatgatgcg
    28441 ttacgccagg gaatgccggt gacggtgcaa ttcggtaacg aggcaggaca tgaatgatgc
    28501 cgttatcacg ctgaacggcc tggaaaaacg ctttccgggc atggacaagc ccgccgtcgc
    28561 gccgctcgat tgtaccattc acgccggtta tgtgacgggg ctggtggggc cggatggtgc
    28621 aggtaaaacc acgctaatgc ggatgctggc gggattactg aaacccgaca gcggcaatgc
    28681 cacggtgatt ggctttgatc caatcaaaaa cgacggcgcg ctgcacgccg tgctcggcta
    28741 tatgccgcag aaatttggtc tgtatgaaga tctcacggtg atggagaacc tcaatctgta
    28801 cgcggatttg cgcagcgtca ccggcgaggc acgtaagcaa acttttgctc gcctgctgga
    28861 gtttacgtct cttgggccgt ttaccggacg cctggcgggc aagctctccg gtgggatgaa
    28921 acaaaaactc ggtctggcct gtaccctggt gggcgaaccg aaagtgttgc tgctcgatga
    28981 acccggcgtc ggcgttgacc ctatctcacg gcgcgaactg tggcagatgg tgcatgagct
    29041 ggcaggcgaa gggatgttaa tcctctggag tacctcgtat ctcgacgaag ccgagcagtg
    29101 ccgtgacgtg ttactgatga acgaaggcga gttgctgtat cagggggaac caaaagccct
    29161 gacacaaacc atggccggac gcagttttct gatgaccagt ccacacgagg gcaaccgcaa
    29221 actattgcaa cgcgccttga aactgccaca ggtcagcgac ggcatgattc aggggaaatc
    29281 ggtacgtctg atcctcaaaa aagaggccac accagacgat attcgccatg ccgacgggat
    29341 gccggaaatc aacatcaacg aaactacgcc gcgttttgaa gatgcattta ttgatttgct
    29401 gggtggtgcc ggaacctcag aatcgccgct gggcgcaata ttacatacgg tagaaggcac
    29461 acccggcgag acggtgatcg aagcgaaaga actgaccaag aaatttggtg attttgccgc
    29521 caccgatcac gtcaactttg ccgttaaacg tggggagatt tttggtttgc tggggccaaa
    29581 cggcgcgggt aaatcgacca cctttaagat gatgtgcggt ttgctggtgc cgacttccgg
    29641 ccaggcgctg gtgctgggga tggatctgaa agagagttct ggtaaagcgc gccagcatct
    29701 cggctatatg gcgcaaaagt tttcgctcta cggtaacctg acggtcgaac agaatttacg
    29761 ctttttctct ggtgtgtatg gcttacgcgg tcgggcgcag aacgaaaaaa tctcccgtat
    29821 gagcgaggcg ttcggcctga aaagtatcgc ctcccacgcc accgatgaac tgccattagg
    29881 ttttaaacag cggctggcac tggcctgctc gctgatgcat gaaccggaca ttctgtttct
    29941 cgacgaaccg acgtccggcg ttgatccgat tactcgccgc gaattctggc tacatatcaa
    30001 cagcatggta gagaaaggcg tcacggtgat ggtcaccacc cactttatgg atgaagcaga
    30061 atattgcgac cgcatcggcc tggtgtaccg tgggaaatta attgccagcg gcacgccgga
    30121 tgatttgaaa gcgcagtcgg ccaacgacga gcaacccgat cccaccatgg agcaagcctt
    30181 tattcagttg atccacgact gggataagga gcatagcaat gagtaacccg atcctgtcct
    30241 ggcgtcgcgt acgggcgctg tgcgttaaag agacgcggca gatcgttcgc gatccgagta
    30301 gctggctgat tgcggtagtg atcccgctgc tactgctgtt tatttttggt tacggcatta
    30361 acctcgactc cagcaagctg cgggtcggga ttttactgga acagcgtagc gaagcggcgc
    30421 tggatttcac ccacaccatg accggttcgc cctacatcga cgccaccatc agcgataacc
    30481 gtcaggaact gatcgccaaa atgcaggcgg ggaaaattcg cggtctggtg gttattccgg
    30541 tggattttgc ggaacagatg gagcgcgcca acgccaccgc accgattcag gtgatcaccg
    30601 acggcagtga gccgaatacc gctaactttg tacaggggta tgtcgaaggg atctggcaga
    30661 tctggcaaat gcagcgagcg gaggacaacg ggcagacttt tgaaccgctt attgatgtac
    30721 aaacccgcta ctggtttaac ccggcggcga ttagccagca cttcattatc cccggtgcgg
    30781 tgaccattat catgacggtc atcggcgcga ttctcacctc gctggtggtg gcgcgagaat
    30841 gggaacgcgg caccatggag gctctgctct ctacggagat tacccgcacg gaactgctgc
    30901 tgtgtaagct gatcccttat tactttctcg ggatgctggc gatgttgctg tgtatgctgg
    30961 tgtcagtgtt tattctcggc gtgccgtatc gcgggtcgct gctgattctg ttttttatct
    31021 ccagcctgtt tttactcagt accctgggga tggggctgct gatttccacg attacccgca
    31081 accagttcaa tgccgctcag gtcgccctga acgccgcttt tctgccgtcg attatgcttt
    31141 ccggctttat ttttcagatc gacagtatgc ccgcggtgat ccgcgcggtg acgtacatta
    31201 ttcccgctcg ttatttcgtc agcaccctgc aaagcctgtt cctcgccggg aatattccag
    31261 tggtgctggt ggtaaacgtg ctgtttttga tcgcttcggc ggtgatgttt atcggcctga
    31321 cgtggctgaa aaccaaacgt cggctggatt agggagaaga gcatgtttca tcgcttatgg
    31381 acgttaatcc gcaaagagtt gcagtcgctg ctgcgcgaac cgcaaacccg cgcgattctg
    31441 attttacctg tgcttattca ggtgattctg ttcccgttcg ccgccacgct ggaagtgact
    31501 aacgccacca tcgccatcta cgatgaagat aacggcgagc attcggtgga gctgacccaa
    31561 cgttttgccc gcgccagcgc ctttactcat gtgctgctgc tgaaaagccc acaggagatc
    31621 cgcccaacca tcgacacaca aaaggcgtta ctactggtgc gtttcccggc tgacttctcg
    31681 cgcaaactgg ataccttcca gaccgcgccg ttgcagttga tcctcgacgg gcgtaactcc
    31741 aacagtgcgc aaattgccgc caactacctg caacagatcg tcaaaaatta tcagcaggag
    31801 ctgctggaag gaaaaccgaa acctaacaac agcgagctgg tggtacgcaa ctggtataac
    31861 ccgaatcttg actacaaatg gtttgtggtg ccgtcgctga tcgccatgat caccactatc
    31921 ggcgtaatga tcgtcacttc actttccgtc gcccgcgaac gtgaacaagg tacgctcgat
    31981 cagctactgg tttcgccgct caccacctgg cagatcttca tcggcaaagc cgtaccggcg
    32041 ttaattgtcg ccaccttcca ggccaccatt gtgctggcga ttggtatctg ggcgtatcaa
    32101 atccccttcg ccggatcgct ggcgctgttc tactttacga tggtgattta cggtttatcg
    32161 ctggtgggat tcggtctgtt gatttcatca ctctgctcga cacaacagca ggcgtttatc
    32221 ggcgtgtttg tttttatgat gcccgccatt ctcctttccg gttacgtttc tccggtggaa
    32281 aacatgccag tatggctgca aaacctgacg tggattaacc ctattcgcca ctttacggac
    32341 attaccaagc agatttattt gaaggatgcg agtctggata ttgtgtggaa tagtttgtgg
    32401 ccgctactgg tgataacggc cacgacaggg tcagcggcgt acgcgatgtt tagacgtaag
    32461 gtgatgtaac ttcttatctt tcgccagcaa agacactacc gccgggccgg caaggattgc
    32521 cagccctgca attgccagca aaaagttgtt ttccagtaca cgggacaaca gccacagcac
    32581 aatcagcgct gcgccaaaat accaggtcgt ggtcagctct tccagcacgt ctgccacctc
    32641 acgccagcgt tgctcggggt ggcgctgaaa caccaacatc atcactaccg tgacacaata
    32701 cagtgcgcat aatcccacgc cgacgtgaac cagagtctta ctcatccagc ccaccaccag
    32761 cagcgccact accagtaaat gcaacataat ctgccagcaa cttagaccag ttgcgacacg
    32821 aacacgttgt tgccacttca tggcttctct cctgaagaat ttatctctac ttactcagag
    32881 taccgaactt tacggaaaat tccgtaagac cgcacctttt tgtgacgccg actacactat
    32941 ttcaatgaga taagaggcaa agacaggaaa attatgcccg atcaaacaca acaattttcg
    33001 ttcaaggtgc tcaccatcaa cattcacaaa ggttttaccg cgtttaaccg acgcttcatt
    33061 ttgccggaac ttcgcgacgc ggtgcgtacc gtcagcgccg atattgtttg cctgcaggaa
    33121 gtgatgggcg cgcacgaagt tcatccgctg catgtggaaa actggcccga tacctcgcac
    33181 tacgagtttc tcgccgacac gatgtggagc gattttgcct acggtcgcaa tgccgtatac
    33241 ccgaaagggc atcacggcaa cgccgtactg tcgcgttatc ccattgaaca ttatgagaat
    33301 cgcgatgttt cggtcgatgg tgcggaaaag cgcggcgtgc tctactgccg cattgtgcca
    33361 ccgatgaccg gaaaagcgat tcatgtgatg tgcgtacatc tgggcctgcg tgaggcgcac
    33421 cgtcaggcgc agcttgcgat gctcgccgaa tgggtgaatg agctaccgga cggcgaaccg
    33481 gtattggtgg cgggtgattt caatgactgg cggcaaaaag ctaatcatcc gttaaaagtg
    33541 caggccggac tggatgagat ttttacccgc gcccacggac gcccggcgcg cacgtttccg
    33601 gtgcaatttc ctctactacg actggacagg atctacgtca aaaatgccag cgccagcgcg
    33661 ccaaccgcgt tgccgctgcg gacatggcga cacctttctg atcatgcccc tttaagtgcg
    33721 gagattcatt tatgaaatgt agctggcgcg aaggcaataa gatccagttg ctggaaaacg
    33781 gcgagcaata ttatcccgcg gtgtttaagg cgattggcga ggcacaagaa cgcatcattc
    33841 ttgaaacgtt tatctggttt gaggatgacg tcggcaaaca gctgcatgcg gcactactgg
    33901 cagcagcgca acgcggggtt aaagcggaag tcttgctgga tggctacggt tcgccggatc
    33961 tcagcgatga gtttgtcaat gaactgacgg cagctggcgt ggtatttcgc tactacgatc
    34021 cccgccctcg cctttttggt atgcgcacca atgtgtttcg ccggatgcat cgcaaaattg
    34081 tggtgatcga cgcgcgtatc gcctttattg gcgggctgaa ttactccgcc gagcatatgt
    34141 ccagctacgg tccagaggct aaacaggatt acgcggtacg ccttgaaggg ccgattgtcg
    34201 aagatattct ccagtttgag ctggaaaacc tgcctggaca gagcgcggca cgacgctggt
    34261 ggcgacgtca tcacaaagcg gaagagaacc gccagccggg agaagcgcag gtattgctgg
    34321 tctggcgcga taacgaagaa catcgcgatg atattgaacg tcactatctg aaaatgctca
    34381 ctcaggcgcg gcgagaagtc attatcgcca acgcctactt cttccccggc tatcgatttt
    34441 tacacgcctt gcgtaaagcg gcacggcgcg gggtgcggat caaactgatc attcagggcg
    34501 aaccggatat gccgattgtc agagtcggtg cgcgcttgct gtataactat ctggttaaag
    34561 gcggcgttca ggtatttgag taccgccgcc gcccgctcca cggcaaagtg gcattgatgg
    34621 acgatcactg ggcgacagta gggtccagta atctcgatcc gctcagtttg tcactgaatc
    34681 tcgaagcaaa tgtcatcatc cacgatcgtc attttaacca gacgctgcgc gataatctga
    34741 acggcattat tgccgcagat tgtcagcagg tcgatgagac catgctgccg aaacgcacct
    34801 ggtggaacct gaccaaaagc gtgctggcgt tccacttttt acgccacttc ccggcgctgg
    34861 ttggctggct tccggcacac acaccacgtc tggcgcaggt tgatccgccc gcacaaccga
    34921 caatggaaac gcaggatcgg gtagaaactg aaaacacggg ggtaaaaccc tgatgagtaa
    34981 atcacacccg cgctggcgct tagcaaagaa gatcctcacc tggctgtttt ttatcgcggt
    35041 gattgtgttg ctggtggtct acgccaaaaa agtggactgg gaagaggtct ggaaggtcat
    35101 ccgcgactac aatcgcgttg cgctgcttag tgcggtcggg ctggtggtcg tcagctatct
    35161 gatttacggc tgctatgacc tgctcgcccg cttttactgc ggtcacaaac tggcgaagcg
    35221 ccaggtgatg ctggtgtcgt ttatctgcta cgccttcaac ctgacgctca gtacctgggt
    35281 cggcggcatt ggtatgcgct atcgtttgta ctcccgacta gggttaccgg gcagcactat
    35341 tacgcggatt ttctcgctca gtattaccac caactggctg ggctatattt tactggcagg
    35401 gattatcttt accgcaggcg tggtggagtt gccggatcac tggtatgtcg atcaaactac
    35461 gctgcgtatt ctcggcattg gcttactgat gattatcgcg gtttatttgt ggttttgcgc
    35521 tttcgcgaag caccgccata tgaccatcaa agggcaaaag ctggtgctgc cttcatggaa
    35581 attcgccctc gctcagatgc tgatttccag cgttaactgg atggtaatgg gggcgattat
    35641 ctggctgtta cttggtcaaa gcgtgaacta tttctttgta ctgggcgtgt tactggttag
    35701 tagtattgct ggcgtcatcg tgcatattcc ggtggggatc ggtgtgctgg aagcggtgtt
    35761 tatcgcacta ctggctgggg agcatacctc gaaagggagc attatcgccg ccctgctcgc
    35821 ttatcgcgtc ctgtattact ttatcccgct gctgctggcg ctgatctgtt acctgctact
    35881 ggaaagccag gcgaagaagc tacgggcgaa aaatgaagcg gcgatgtgaa gtttagttaa
    35941 gttctttagt atctgcattt acgactaatg aaaaaaacgc gtatgccttt gccagacaag
    36001 cgttatagct atcgcggcaa gtatcccaaa gaagccgata aaatagtaat agagtgttaa
    36061 tgcacctgta acagcgcaac gaaccggact ccacaactct tcactatggc atttccccgc
    36121 caattcaagg agcttatttc gtccatgcga gataatgcct gaccataaca caatcattaa
    36181 gcaacttatt acccaaacgg gtcgttcact cattaatgcg gcattcatga tgatcaccag
    36241 ggccaaccct gtcaacgtca tcataatgat aaaacggtga gaaccaggat caacattaaa
    36301 taataaaccg gccagcatac tgattaaaaa catcgcgccc gcagtgctga acacgacgat
    36361 aatggcaggt acaggagcta aacttgcaac gacaatcgcc accaaagaga gtaaaaacag
    36421 atatattacg cctgcgatga cggcatttat cttgttgata tccttactta agccatacat
    36481 acaaactaat aataacccta tggcgacata cattgttggt gaatcatatt tggttgccgt
    36541 aatttcgaga tacttagctc cccaggccag tcctgcggtt gccaccaaac ctaaagcggt
    36601 aatgagccat ataccgttta atatcctggt ctcgtgctga aaatctaatt tattgctgtt
    36661 ttgtgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
    36721 ataaatttaa aaccacgcat acaaaatact tttgtattta tggatacgga aaacgcttcc
    36781 gctcgccata aggcgtattt tatacttggt acttgttaag tgtcgttttt agtaagaaaa
    36841 aaaatcccga cacatggccg ggatattgat ggtgaaaaag aattaacggc ggttgccaaa
    36901 aattcgcagc aacatcagga acaggttgat gaagtccaga tacaaggtta acgcgccaag
    36961 aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac ccatattttt
    37021 caatttctga gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac
    37081 tgcccacatc aatgcttcgc ttttcagcca gaagttgacc agcgatgcca gcacaatgcc
    37141 gattaacgcc ataaacagca tattgccaaa gccacttaaa tcgcgcttcg tggtgtaacc
    37201 gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg cgatagaagc
    37261 agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg aataaagcat
    37321 aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac
    37381 caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtaa acaacagctc
    37441 catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca acaagccaac
    37501 ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg gttgtacgat
    37561 tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa
    37621 ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt
    37681 gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag cgttttgccg
    37741 cctgctgatc gctctcccga gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct
    37801 tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct tcaaacgcac
    37861 tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc cataattccc
    37921 cgatgcggtg aatcacagtg acgcgcccca gcggccagcg gttacgcgct tcatcaacaa
    37981 tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat gctttgacgc
    38041 tgtcgcccag gttatggttg cgcaccttac cagtaaaggt gactaccgca ccgtcttcgt
    38101 cacgctccgc cagccacggg tactcttctc ctacgctgaa cggctgcgga ccaacaacaa
    38161 ttttggtttc tgccatctta acctccggtt accggcggga agaaagctac ttcgtcgccg
    38221 tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag taatttgcca
    38281 tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca tgtgctggcg taacgcttca
    38341 acagttggga aatccgcagc cacttcggtt gcatctgttc ccaccaactc gcgcacctgg
    38401 gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact tgccgccgct
    38461 cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt tgcacatgtc
    38521 ataaatggtc agcgccgtca cggaggccgc ggttaatgct tccatttcga caccggtttt
    38581 cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct cggcctgtaa
    38641 attgacttca actttgctga gcatcagcgg atgacagagc gggatcagat cccaggtgcg
    38701 ttttgccgcc tgaataccgg caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg
    38761 accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc
    38821 ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc cagcggcgtt
    38881 gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt acacggacgc
    38941 gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca cgctttggtc
    39001 gaacccggca tggcgaaaat cagcgttttg ttggcgacgc ccgctaccgc acgagattgc
    39061 aacgtggaag tgccaatctc ttcaaacgac aacatacgga acacttcacc aaaaccttca
    39121 atttcacggt cgaacaacgg cagcaatgct tcgggagcct gatcaccttc cgtcaggcca
    39181 gtaccacccg taatcaatac cacttgtaca tcgtcgctgg cgatccacgc agatacctga
    39241 gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg atggcccgct
    39301 tcttgcgccg aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc gcgacgatta
    39361 gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg actcatctga
    39421 tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat accggtgttg
    39481 ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc
    39541 agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc gccttcacca
    39601 aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca agtggcgcag
    39661 aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg atggcaaaag
    39721 acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg acgcagtagc
    39781 tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct gccctcgccc
    39841 gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca gttcagaaag
    39901 gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt gaccttctca
    39961 aaaccggcct caaatgcagc atcaatcccc gccatgacct ggttgaattt atcctgcccg
    40021 gtaatagcgt gaaactggcg ggcgtccaga ctgtcgacac tgacgttaat gccagtaagt
    40081 cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt ggtgaccgca
    40141 atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt
    40201 aaagacggct caccgcctgt caggcgcact ttttcggtgc ccagactggc gaaggcgcgc
    40261 gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc gctcggtttg
    40321 tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat cgacaggcgc
    40381 aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta cacctttcca
    40441 gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac gagcattgtc
    40501 acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt agtggttatg
    40561 ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgt aatcatgtat
    40621 atagcgtcat gatcgtgcta tttcgctaca gaataaggga aaatggcggt tttactttga
    40681 tatacatcat cagggaggca tagcgtgcat cgtcctttag aggtagaaga aaatagtttg
    40741 tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg
    40801 ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg
    40861 ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg tctttgggtt
    40921 ctcgtttaac gggtatcgtc accaccaccg ataatggtgg ctcgacgggg cgtattcgcc
    40981 gttcagaagg cggcattgcc tggggcgata tgcgcaactg cctcaaccag ctgataacgg
    41041 aaccgagcgt cgcctccgcg atgtttgaat accgttttgg tggcaatggc gaactttccg
    41101 gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg cggcctctgg
    41161 aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt ccaatgtcag
    41221 agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac ggcgaggtca
    41281 atatcgacca gttaactacg ccgattcaag agttattgtt aacgcctaat gtacccgcaa
    41341 cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg cctggcagtt
    41401 tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca ttacgccgca
    41461 cgccagcgcc gatggtttat atcggcaatc tggggcgtga gttgagttta cctgcggcta
    41521 atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa aaggtcattg
    41581 atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg attgtgatcc
    41641 aggaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg ttacataacg
    41701 cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc
    41761 ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc
    41821 tacaaaccgc gcaaattcaa tatattgcgg agaaaatgta ggcctgataa gcgtagcgca
    41881 tcaggctgtt ttccgtttgt catcagtctt cttcgctatc ctgttacgat gccgcgataa
    41941 acagctcacg cagctgatgc aactggtcac gaatttgcgc cgcttcttcg aactccagat
    42001 tctgcgcgtg ttgcatcatc aacccttcca gctcatggat tttctgctgc aacgctttag
    42061 gcgacatatc catcggcaca ttatccggct caacaatcgg tcgcgatttt cctctgccct
    42121 tcgctttggt tttagcaatg ttctgcccca acgccaggat atcgaccact ttcttgttca
    42181 agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctcg cggcgacgtt
    42241 cggtttcgcc aatcgctttc gccattgatg gggtgatctt atcgccgtag agaatcgctt
    42301 taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca
    42361 ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga aacttccggc atatccagac
    42421 cttcacgcag taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga
    42481 taatctccat acgctcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt
    42541 gttcttcgag atattcggta agatcttccg ccattcgctt ggtcagtgtg gtgaccagta
    42601 cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca acctgtgtcg
    42661 ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc acctgatcca
    42721 ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg
    42781 tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acggttatcc agcgctgatg
    42841 gcaggcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg cgatacatgc
    42901 cgccaatttg tggaatggtg acgtgagatt catcgaccac cagcagccca tcagcaggga
    42961 ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg aagcgcgagt
    43021 agttttcaat ccccgaacag tagcccagct cgttcatcat ctccagatca aactgggtac
    43081 gctgggtcag ccgctgctct tccagcagtt tgttgttttc caacagcact ttgcgtctgg
    43141 cggccagctc ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt
    43201 agtgcgtttt cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg
    43261 ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag ttccacgcga agtgcaatgt
    43321 catccgattc tgccgggaag atatctatca cctcgccacg aacgcggaaa gtaccacgct
    43381 ggaatgcttg atcattacga gcgtattgca gctccgccag tcggcgcaga atcgcgcgct
    43441 gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa tcaggatcgc
    43501 ccagaccata aatcgcggaa acagacgcca ccacaaccac atcacgccgc tccagcatcg
    43561 ctttggtggc ggacaaacgc atctgctcaa tatgttcgtt aaccgaggca tctttctcaa
    43621 tgaaagtgtc ggaactcggt acataggctt ccggctgata gtagtcgtag taggaaacga
    43681 aatattccac cgcgttttcc gggaagaact ctttcatttc gccatacagc tgggccgcca
    43741 gcgttttgtt gggcgcaagt accatggttg ggcgctgaag gtcagcaatg acattggcaa
    43801 tggtgaatgt tttcccggag ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt
    43861 ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg
    43921 aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc gggcaggtat
    43981 gtaattttac tcgtcgtact taattatgcc aacaaattat actggataaa aaaacagttc
    44041 atcaccataa tatttctgtt acagcgtaaa ctccgctgtc aatcatgagc aaaatttact
    44101 ctgtggcgag ataaaactcc ggccttaccg ggttatcccc aaagcaacgg cttttttaac
    44161 atttgtcaag atgagtgatg acagttttat ggcaagagat gcctgttcag tgactgccat
    44221 tgcatttatc taaccagtta aaaaataaaa gatatttctt tgagccgtct ttaacgccaa
    44281 ttcgcgtgca agccgcgtat tctctcgctt gcctcgtgtt ttctaactct aatacacatg
    44341 gttatccaca ggaatagtgg ataactgtct ccagccccta tcccccgccg cctgggcaat
    44401 tcccacattc gccgcatgag gcggaagtaa aatttttttc gtgattattt tagaatttaa
    44461 ttggttaaat tgcagtcaat cgaagacgcg atctcgctcg caatttaacc aaatacagga
    44521 tagctacaac aaggcaaggt ttatgtactt tccggttgcc gcattttctg gattttctgc
    44581 aagccagggg atctctccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata
    44641 ttcagcgtga cgttttcccg gaggcgtaac atcgttcgcc acccaacccg ccagagtcag
    44701 tccggcgtgt tgtattgcct gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac
    44761 accaactacc agtatcaccg gcagttgttc ctgtgttacc caatctgcaa aagtgaaagt
    44821 gtcagaaagc ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg
    44881 ttcaagcgcg cgtaatccgg cgctcattac caatgattct atcggcctgc cctcttgcgc
    44941 gctgatgatg tgcggcgagg tgggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc
    45001 cagctgcagg ctgctgttgt gctgtaacgc cagcgcgtca ctattacgta agccttccgg
    45061 ggtcttttcg ctgccagagg cgaccggttt ataacctgcc gtccgataac ctactgcctt
    45121 cgcggcttgt aaaagtgcac aactggcgac ggttttcccc acttcggtat ccgttccggt
    45181 gacaaaataa cgtttactca cgagcaatca ctcccaaaaa aagatgatac gtcagaggat
    45241 atcgcccctg ttgttgcggc caggccagtt gcaatcgctg caattgcgaa cgcgttaata
    45301 ttcgcgggtc gcgcccttca tgaagatgcg tagcaccgat gcctttcagc gaacgcatgg
    45361 cactgagcgc atcatcaaac catagtgtga tgggctgaat atgatgttga taatgcacgc
    45421 cgttcagcga ctgttcgatt tcatctggcg gtaaaaagcg attagcatgc ggacgctcgt
    45481 ccaccgcctg ccacgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg
    45541 ccaccacgcc tccggggcgc accacccgat acagctcgcg gagtgccgtg gataaattac
    45601 cgcaccactg cactgcgaga ttgctccatg caagatcgaa cgtcgcagtc gctaacggca
    45661 gggattcgat atctcccgcc agataatggt ctgcggcatc cttctggcgt gcctgaacaa
    45721 gcattggcgg cgagagatct aaggccgtca cctgcgcgtg acgttcccgc cagtggcggc
    45781 tcatccagcc aggtccacaa cccgcgtcca gtacgtgggt gtatttacgc tgtgggagca
    45841 ttgccagtaa gacgtcagca ctctggcgct gtagatctgc gtgttgctca tagagtgcgg
    45901 ctgcccgacc aaatgccgct gcaatggctt gtttattaac cgttgccatg cagcacctcc
    45961 agcagacggt cgatatcctg catttcatgc gctgcggtta gcgttaagcg cagtcgcgca
    46021 gtaccagcgg gtacggttgg cgggcgaatc gccgtgaccc agcagccttg ctggcgcagt
    46081 ttttctgcca gttgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgctg
    46141 catgaatcag ccagcgtaaa cggcaaatcc tgtactccgg cacgaaaacg cgcaatgagt
    46201 gacaccagtt tttcgcgccg tgcatccccc tcatcacggc gaatgaccgc cagtgacgca
    46261 cgtaatgcct gcgcctgagc gggcggcata ctggtgctgt agataaggtg gcgggcgaat
    46321 tgcagcagat aatccgccac cgtactggag caaagcaccg cagccccact gacgccaaat
    46381 cctttgccaa aagtcaccac cagcaattct ggttttacct tttgcagcca gcagctgccg
    46441 cgcccctgct ccccgataac gcccgtgccg tgggcatcat cgaccatcaa ccagccattg
    46501 tgctgttgcg ttacctgctg gatttccgcc agtggcgcaa tatcgccgtc catgctgaac
    46561 acgccttctg tcaccaccag ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga
    46621 tgagtgacat cgttatgaac aaaacggcga agctgcgacg ggcttaaact ggcagcctcc
    46681 agcaatgagg catggctaag ccggtcagcg acaatgcggt cctcttttgc catcatcgcg
    46741 gcaatgaccg cctgattagc ggcaaaacca gagataaata gcagtgcccg cgaatagccc
    46801 agccactcgg ccagttcttc ttccagcgcc tgatgcgcaa cgctataacc gctgacgtga
    46861 ccggaaccgc cgctaccgac gccaaattgc tccgcaccct gctgccaggc acggataatt
    46921 tgcggatgat ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcgatca
    46981 tccgccacca gccagcgtcc ggctccttgc gccactggat aacggcgacg cagggcatcg
    47041 gcagcacgcc gcgcatcgag cgccgcgttg attttctcct gccagctcat aatgctgccg
    47101 cgttgtaata ttcgtcggtg tccggggtca tcagcgcctg ttcaagacgt tgctgttgtt
    47161 cgttatcccc tgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt
    47221 gcaggtcttt atcttcttcc ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg
    47281 agtttgcgcc tgccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg
    47341 cagaaaggcg cacgtaagag gtcggcatca tgatccgcgc gaccgcaatg gtgcgaataa
    47401 aatcaaaggc atcgacatca tcgttatccg ccagcggcgt gcctttcacc ttcaccagca
    47461 tgttgattgg tacgctttcc ggcggcgtcg gcaggtttgc cagttgcagc aataatccgg
    47521 cgcgatcttt taccgtttcg cctaagccca caatgccgcc agaacagact ttgatcccgg
    47581 catcgcgcac tttttccagc gtatcgaggc gttcctgata agtgcgggtg gtgatgatat
    47641 tgccgtaaaa ctccggcgag gtgtcgaggt tgtggttgta gtaatccagc ccggcattcg
    47701 caaggcgctg cgcctgagat tcactcaacg tgcccagcgt catacacgcc tccagcccca
    47761 tcgcttttac cccctgcacc atttgttcta ggtacggcat atcgcgttcg tggggattct
    47821 tccacgccgc gcccatacag aaacgcgtcg atcccgcagc tttcgctttg cgcgccgact
    47881 ccagcacctg ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc
    47941 tttgcgggca gtatttgcaa tcttccggac aagctccggt cttaatcgac agcaaagtgc
    48001 tgacctgcac ctgacgagga tcgaaatgct gtcgatgcac ctgctgcgct tcaaacagca
    48061 gatccagcaa cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt
    48121 gagccatggg gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag aattgtaaac
    48181 ctaaatcttt tcaatttggt ttacaagtcg attatgacaa cggacgatct tgcctttgac
    48241 caacgccata tctggcaccc atacacatcc atgacctccc ctctgccggt ttatccggtc
    48301 gcgagcgccg aaggttgcga gctaattttg tctgacggca gacgcctggt tgacggtatg
    48361 tcgtcctggt gggcggcgat ccacggctat aatcacccgc agcttaatgc ggcgatgaag
    48421 tcgcaaattg atgctatgtc gcatgtgatg tttggcggta tcacccatgc gcccgccatc
    48481 gagctatgcc gcaaactggt ggcaatgacg ccgcaaccgc tggaatgcgt ttttctcgcg
    48541 gactccggct ccgtagcggt ggaagtggcg atgaaaatgg cgttgcagta ctggcaggcc
    48601 aaaggcgaag cgcgccagcg ttttctgacc ttccgcaatg gttatcatgg cgataccttt
    48661 ggcgcgatgt cggtgtgcga tccggacaac tcaatgcaca gcctgtggaa aggctatctg
    48721 ccagaaaact tgtttgctcc cgccccgcaa agccgcatgg atggtgaatg ggatgaacgt
    48781 gatatggttg gctttgcccg cctgatggcg gcgcatcgtc atgaaatcgc ggcggtgatc
    48841 attgaaccga ttgtccaggg cgcaggcggg atgcgcatgt accatccgga atggttaaaa
    48901 cgaatccgca aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc
    48961 ggatttggtc gtaccggcaa actgtttgcc tgtgaacatg cagaaatcgc gccggacatt
    49021 ttgtgcctcg gtaaagcctt aaccggcggc acaatgaccc tttccgccac gctcaccacg
    49081 cgcgaggttg cggaaaccat cagtaacggc gaagccggtt gctttatgca tgggccaact
    49141 tttatgggca atccgctggc ctgcgcggca gcaaacgcca gcctggcgat tctcgaatct
    49201 ggcgactggc agcaacaggt ggcggctatt gaagtacagc tgcgcgagca acttgccccc
    49261 gcccgtgatg ccgaaatggt tgccgatgtg cgcgtgctgg gggcaatcgg cgtagtcgaa
    49321 accactcatc cggtgaatat ggcggcgctg caaaaattct ttgtcgaaca gggtgtctgg
    49381 atccggcctt ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag
    49441 ttgcagcgtc tgaccgcagc ggttaaccgc gcggtacagg atgaaacatt tttttgccaa
    49501 taacgagaag tccgcgtgag ggtttctggc tacactttct gcaaacaaga aaggagggtt
    49561 catgaaactc atcagtaacg atctgcgcga tggcgataaa ttgccgcatc gtcatgtctt
    49621 taacggcatg ggttacgatg gcgataatat ttcaccgcat ctggcgtggg atgatgttcc
    49681 tgcgggaacg aaaagttttg ttgtcacctg ctacgacccg gatgcgccaa ccggctccgg
    49741 ctggtggcac tgggtagttg ttaacttacc cgctgatacc cgcgtattac cgcaagggtt
    49801 tggctctggt ctggtagcaa tgccagacgg cgttttgcag acgcgtaccg actttggtaa
    49861 aaccgggtac gatggcgcag caccgccgaa aggcgaaact catcgctaca tttttaccgt
    49921 tcacgcgctg gatatagaac gtattgatgt cgatgaaggt gccagcggcg cgatggtcgg
    49981 gtttaacgtt catttccact ctctggcaag cgcctcgatt actgcgatgt ttagttaatc
    50041 actctgccag atggcgcaat gccatctggt atcacttaaa ggtattaaaa acaacttttt
    50101 gtctttttac cttcccgttt cgctcaagtt agtataaaaa agctgaacgc gaaacgctaa
    50161 agaccattaa tatcaatgtg ttacaatata ttt
//
LOCUS       DAZGOB010000020        48011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGOB010000020 DAZGOB010000000
VERSION     DAZGOB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.020"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            179..763
                     /gene="yadN"
                     /locus_tag="ACHLIF_001210"
     CDS             179..763
                     /gene="yadN"
                     /locus_tag="ACHLIF_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9605262.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            868..1608
                     /gene="yadV"
                     /locus_tag="ACHLIF_001211"
     CDS             868..1608
                     /gene="yadV"
                     /locus_tag="ACHLIF_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO9605263.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1643..4243
                     /gene="htrE"
                     /locus_tag="ACHLIF_001212"
     CDS             1643..4243
                     /gene="htrE"
                     /locus_tag="ACHLIF_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO9605264.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4260..4829
                     /gene="yadM"
                     /locus_tag="ACHLIF_001213"
     CDS             4260..4829
                     /gene="yadM"
                     /locus_tag="ACHLIF_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9605265.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLSGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4844..5446
                     /gene="yadL"
                     /locus_tag="ACHLIF_001214"
     CDS             4844..5446
                     /gene="yadL"
                     /locus_tag="ACHLIF_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO9605266.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5473..6069
                     /locus_tag="ACHLIF_001215"
     CDS             5473..6069
                     /locus_tag="ACHLIF_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO9605267.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            6053..7396
                     /locus_tag="ACHLIF_001216"
                     /pseudo
     CDS             6053..7396
                     /locus_tag="ACHLIF_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            7509..8303
                     /gene="panB"
                     /locus_tag="ACHLIF_001217"
     CDS             7509..8303
                     /gene="panB"
                     /locus_tag="ACHLIF_001217"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO9605268.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8315..9166
                     /gene="panC"
                     /locus_tag="ACHLIF_001218"
     CDS             8315..9166
                     /gene="panC"
                     /locus_tag="ACHLIF_001218"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO9605269.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIIRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9240..10142)
                     /gene="rpnC"
                     /locus_tag="ACHLIF_001219"
     CDS             complement(9240..10142)
                     /gene="rpnC"
                     /locus_tag="ACHLIF_001219"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO9605270.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10416..10796
                     /gene="panD"
                     /locus_tag="ACHLIF_001220"
     CDS             10416..10796
                     /gene="panD"
                     /locus_tag="ACHLIF_001220"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO9605271.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10800..12029)
                     /gene="yadE"
                     /locus_tag="ACHLIF_001221"
     CDS             complement(10800..12029)
                     /gene="yadE"
                     /locus_tag="ACHLIF_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO9605272.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12093..12533)
                     /gene="yadI"
                     /locus_tag="ACHLIF_001222"
     CDS             complement(12093..12533)
                     /gene="yadI"
                     /locus_tag="ACHLIF_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO9605273.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12638..13408)
                     /gene="yadH"
                     /locus_tag="ACHLIF_001223"
     CDS             complement(12638..13408)
                     /gene="yadH"
                     /locus_tag="ACHLIF_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9605274.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13405..14331)
                     /gene="yadG"
                     /locus_tag="ACHLIF_001224"
     CDS             complement(13405..14331)
                     /gene="yadG"
                     /locus_tag="ACHLIF_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9605275.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14440..15102
                     /gene="can"
                     /locus_tag="ACHLIF_001225"
     CDS             14440..15102
                     /gene="can"
                     /locus_tag="ACHLIF_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO9605276.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15143..15679)
                     /gene="hpt"
                     /locus_tag="ACHLIF_001226"
     CDS             complement(15143..15679)
                     /gene="hpt"
                     /locus_tag="ACHLIF_001226"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO9605277.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15885..18275
                     /gene="gcd"
                     /locus_tag="ACHLIF_001227"
     CDS             15885..18275
                     /gene="gcd"
                     /locus_tag="ACHLIF_001227"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO9605278.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18322..19872)
                     /gene="cueO"
                     /locus_tag="ACHLIF_001228"
     CDS             complement(18322..19872)
                     /gene="cueO"
                     /locus_tag="ACHLIF_001228"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO9605279.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20038..20385
                     /gene="yacC"
                     /locus_tag="ACHLIF_001229"
     CDS             20038..20385
                     /gene="yacC"
                     /locus_tag="ACHLIF_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO9605280.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20491..21357
                     /gene="speE"
                     /locus_tag="ACHLIF_001230"
     CDS             20491..21357
                     /gene="speE"
                     /locus_tag="ACHLIF_001230"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO9605281.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21373..22167
                     /gene="speD"
                     /locus_tag="ACHLIF_001231"
     CDS             21373..22167
                     /gene="speD"
                     /locus_tag="ACHLIF_001231"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO9605282.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22205..22567)
                     /gene="yacL"
                     /locus_tag="ACHLIF_001232"
     CDS             complement(22205..22567)
                     /gene="yacL"
                     /locus_tag="ACHLIF_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO9605283.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22742..25339)
                     /gene="acnB"
                     /locus_tag="ACHLIF_001233"
     CDS             complement(22742..25339)
                     /gene="acnB"
                     /locus_tag="ACHLIF_001233"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO9605284.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHSL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25694..27172
                     /locus_tag="ACHLIF_001234"
                     /pseudo
     CDS             25694..27172
                     /locus_tag="ACHLIF_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(27423..27953)
                     /locus_tag="ACHLIF_001235"
     CDS             complement(27423..27953)
                     /locus_tag="ACHLIF_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605285.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGESPNDVGICGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLDKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            28144..28392
                     /locus_tag="ACHLIF_001236"
     CDS             28144..28392
                     /locus_tag="ACHLIF_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9605286.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            28394..30484
                     /locus_tag="ACHLIF_001237"
     CDS             28394..30484
                     /locus_tag="ACHLIF_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO9605287.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSKMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVAVQQPAVDDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            30555..31487
                     /locus_tag="ACHLIF_001238"
     CDS             30555..31487
                     /locus_tag="ACHLIF_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9605288.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            31490..31711
                     /locus_tag="ACHLIF_001239"
     CDS             31490..31711
                     /locus_tag="ACHLIF_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605289.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            31724..31978
                     /locus_tag="ACHLIF_001240"
     CDS             31724..31978
                     /locus_tag="ACHLIF_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605290.1"
                     /translation="MNIQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            31980..32261
                     /locus_tag="ACHLIF_001241"
     CDS             31980..32261
                     /locus_tag="ACHLIF_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO9605291.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            32258..32530
                     /locus_tag="ACHLIF_001242"
     CDS             32258..32530
                     /locus_tag="ACHLIF_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605292.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFTELVAMKASALMKDMDMSSGVIKGNEFNQQG"
     gene            32535..32828
                     /locus_tag="ACHLIF_001243"
     CDS             32535..32828
                     /locus_tag="ACHLIF_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605293.1"
                     /translation="MSKVRVIFEFDHVMHEVKPAGNDSEEITEGVTATVKIERDTENR
                     PAGPCDVYAQILKYHSPTIIQFLTDELQGSMQAMGVSSSVERRSVQNGPDTLQ"
     gene            32840..33370
                     /locus_tag="ACHLIF_001244"
     CDS             32840..33370
                     /locus_tag="ACHLIF_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9605294.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            33468..34010
                     /locus_tag="ACHLIF_001245"
     CDS             33468..34010
                     /locus_tag="ACHLIF_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605295.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            34014..34547
                     /locus_tag="ACHLIF_001246"
     CDS             34014..34547
                     /locus_tag="ACHLIF_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605296.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            34547..35062
                     /locus_tag="ACHLIF_001247"
     CDS             34547..35062
                     /locus_tag="ACHLIF_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605297.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            35066..35617
                     /locus_tag="ACHLIF_001248"
     CDS             35066..35617
                     /locus_tag="ACHLIF_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO9605298.1"
                     /translation="MMILQPMGRKGQAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDAVLRELTP"
     gene            35614..35799
                     /locus_tag="ACHLIF_001249"
     CDS             35614..35799
                     /locus_tag="ACHLIF_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605299.1"
                     /translation="MIKRLVFFAIFVPVLGVGFIFAITGNLSMMPDIWFFIRMSLFLF
                     IMNLLIDIYIRITGKHK"
     gene            35838..36170
                     /locus_tag="ACHLIF_001250"
     CDS             35838..36170
                     /locus_tag="ACHLIF_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605300.1"
                     /translation="MSIELRSSYEYRKFLIAGGMKPEDVEKIVSFMDKECDKRDVSEI
                     IMDDMILDSVVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            36163..36360
                     /locus_tag="ACHLIF_001251"
     CDS             36163..36360
                     /locus_tag="ACHLIF_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605301.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            36350..36646
                     /locus_tag="ACHLIF_001252"
     CDS             36350..36646
                     /locus_tag="ACHLIF_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605302.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHVRWDEAEKAFHVRKKVNTV"
     gene            36643..37152
                     /locus_tag="ACHLIF_001253"
     CDS             36643..37152
                     /locus_tag="ACHLIF_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO9605303.1"
                     /translation="MRGKLISAIHVAKRELALDDEAYTFALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMEMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            37222..37647
                     /locus_tag="ACHLIF_001254"
     CDS             37222..37647
                     /locus_tag="ACHLIF_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO9605304.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDILKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            37719..38219
                     /locus_tag="ACHLIF_001255"
     CDS             37719..38219
                     /locus_tag="ACHLIF_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9605305.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            38254..38682
                     /locus_tag="ACHLIF_001256"
     CDS             38254..38682
                     /locus_tag="ACHLIF_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605306.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASVLVPDDVADRVRRAVCEIRACEAGADPR"
     gene            38666..38884
                     /locus_tag="ACHLIF_001257"
     CDS             38666..38884
                     /locus_tag="ACHLIF_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605307.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            38895..39122
                     /locus_tag="ACHLIF_001258"
     CDS             38895..39122
                     /locus_tag="ACHLIF_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9605308.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            39103..39411
                     /locus_tag="ACHLIF_001259"
     CDS             39103..39411
                     /locus_tag="ACHLIF_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO9605309.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            39408..39698
                     /locus_tag="ACHLIF_001260"
     CDS             39408..39698
                     /locus_tag="ACHLIF_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO9605310.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            39701..40282
                     /locus_tag="ACHLIF_001261"
     CDS             39701..40282
                     /locus_tag="ACHLIF_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO9605311.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            40282..41946
                     /locus_tag="ACHLIF_001262"
     CDS             40282..41946
                     /locus_tag="ACHLIF_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605312.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMLRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            41946..43535
                     /locus_tag="ACHLIF_001263"
     CDS             41946..43535
                     /locus_tag="ACHLIF_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO9605313.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLSIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVRDQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPGKQEKTEQTALAARLPEAKS
                     TPRDELDDMGDAVPARLLQEAIDPVLEPVIDAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            43519..44844
                     /locus_tag="ACHLIF_001264"
     CDS             43519..44844
                     /locus_tag="ACHLIF_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO9605314.1"
                     /translation="MAQGIDLGYAATLASKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKAARLDVLTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGNPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFAQLPAMLAAPDAVLWDHVHQNLLYITETRDG
                     TAKIAVNAPYSVKRQPDKLDVVINAYRVSRNTLIDAVQGGKMEILEGKL"
     gene            complement(44844..44931)
                     /locus_tag="ACHLIF_001265"
     tRNA            complement(44844..44931)
                     /locus_tag="ACHLIF_001265"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            44970..45443
                     /locus_tag="ACHLIF_001266"
     CDS             44970..45443
                     /locus_tag="ACHLIF_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO9605315.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            45620..46744
                     /locus_tag="ACHLIF_001267"
     CDS             45620..46744
                     /locus_tag="ACHLIF_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO9605316.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTGLQTD
                     TVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            46744..47691
                     /locus_tag="ACHLIF_001268"
     CDS             46744..47691
                     /locus_tag="ACHLIF_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO9605317.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAINRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGINAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAIKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            47735..>48011
                     /locus_tag="ACHLIF_001269"
     CDS             47735..>48011
                     /locus_tag="ACHLIF_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO9605318.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEEEPCLTVRILQTSADDAGSVAGVVHAVAGTD"
BASE COUNT        12127 a        12070 c        12378 g        11436 t
ORIGIN      
        1 taatttgttc aaaagccctt aaagtgcaca ataggcgtcc taatgaacaa tggttgtaaa
       61 ttttggtaac aaatatcatt gctcattaat atctagcatc tgcttataac atttgtgtca
      121 tgaatagtca ttcttcaaac cagtagtctg actaatggaa ttttattcaa ggatttttat
      181 gtctaaaaaa ttaggttttg ccctgtctgg tcttatgctg gcaatggttg caggtactgc
      241 ttctgctgat atggatggtg gtcagttaaa tatcagtggt ctggttgttg ataacacctg
      301 tgaaacccgc gttgacggcg gtaataaaga tggtctgatc ctgctgcaaa ctgcaactgt
      361 tggtgaaatc gatgctggcg tgctgaatga caccgttggc gctaaagcca aacctttcag
      421 catcaccgtt gactgcagca aagcaaatcc gaatccaggt agtacagcta aaatgacctt
      481 cggttctgtt ttctttggta atagcaaagg aacgctgaac aacgacatgt ctattaacaa
      541 cccgtctgac ggcgtgaata tcgctctgca caatattgat ggttccacta tcaaacaggt
      601 tcaaattaac aaccctggcg atgtgtatac taaagccctg gatgcaacga caaaatctgc
      661 tgtttatgat tttaaagcgt cttacgttcg tgccgtcgca gaccaaacag caactgctgg
      721 ttatgtaaaa actaacactg catacaccat tacttatcag taagtattca ccaattaaat
      781 tgattgaatg aatatacagg gaatagtgtt aataatttct atttttatat tattccctgt
      841 tttaattaac tctgtcaggg atggtttatg ttttttaaca ccaaacatac aacagcttta
      901 tgcttcgtaa cctgtatggc ttttagttca tcgtctattg cggacattgt catttcgggt
      961 actcgcgtaa tatataaaag cgatcaaaaa agtgtcaacg tacgtctgga aaataaaggg
     1021 aataacccgt tgcttgtcca gagttggtta gatactggcg atgacaacgc tgagcctggc
     1081 agtattacag tcccttttac tgccacgccg ccagtatcgc gtattgatgc caaacgtggg
     1141 caaacaatca aattaatgta cacagccagc acctcactgc ctaaagacag agagagcgtg
     1201 ttctggttta acgtactgga agttccacca aaaccagatg cagaaaaggt cgcgaatcaa
     1261 agtctgctgc aactggcatt tcgcacacgt ataaaacttt tctatcgccc ggatggattg
     1321 aagggaaatc cctctgaagc cccgttagcc ctgaagtggt tctggtcagg ttcagaaggt
     1381 aaggcgtcat tgcgagtgac caatccaacc ccttactacg tctcttttag cagtggtgat
     1441 ttagaagcta gcggtaaacg ctatccgatt gatgtgaaaa tgattgcacc atttagtgat
     1501 gaggtcatga aagtcactgg ccttaatggc aaagcaagtt ctgcaaaagt acatttttac
     1561 gccattaatg attttggtgg cgccattgaa ggtaatgcca gtctgtaatc aggcaaggat
     1621 ataattccgc aggaagcata acgtgactat aaaaccaact aaaaattatc ataatcatct
     1681 gacccgtatc gctacgtttt gcgcgctgct gtattgcaat agtgctttct gtgctgaact
     1741 tgttgaatat gaccatacct tcctgatggg gcagaatgcc tcaaatattg atctcagccg
     1801 gtatagtgaa ggtaaccctg ctataccggg tatgtacgac gtcagtgttt atgtcaacga
     1861 tcaacctatt attaaccaaa gcattacatt tattgaaatt gaaggaaaaa aaaacgcaca
     1921 agcctgtatc acacttaaga atttattgca gtttcatata aattctcccg atataaataa
     1981 cgaaaaagcc gttctgcttg ccagggatga aacactcggc aattgcctta atttgacgga
     2041 aattatccct caggcttctg ttcgttatga cgttaacgag caacgtctgg atatagacgt
     2101 tcctcaggca tgggtaatga aaaattacca aaactatgtt gatccatcgt tatgggaaaa
     2161 tggcattaat gcggccatgt tgtcatacaa cctcaacgga tatcatagtg aaactcctgg
     2221 tcgaagaaat gacagcattt atgctgcttt taacggtggg atgaatttag gtgcatggcg
     2281 actgcgtgcc tcaggcaact acaactggat gaccgattct ggcagtaatt atgattttaa
     2341 gaatcggtat atacagcgtg atatcgcctc gctgcgttct caactcattc ttggtgagtc
     2401 ttatacaacg ggcgaaacct ttgattctgt cagtatccgc ggcattcgtt tatacagcga
     2461 cagccgcatg ttgcctccga cattagcgag ttttgcgcct atcattcatg gcgttgctaa
     2521 taccaacgcc aaagtcacta ttacgcaagg tggctataag atttatgaaa cgactgtgcc
     2581 gccaggcgct ttcgtcattg atgatctgag tccgtcaggt tacggcagcg atcttattgt
     2641 tactgtcgaa gaatccgatg gctcaaagcg gacattctcg caaccttttt catcggtcgt
     2701 tcaaatgtta cgccctggcg ttggacgttg ggatattagc ggcggccagg tcttaaaaga
     2761 tgatattcag gatgagccta atttatttca ggccagctac tactacggtc tgaataacta
     2821 tctaacgggt tatactggta ttcagattac cgataataac tataccgctg gtttgttagg
     2881 tcttggtctg aatacttcag tcggtgcatt ttctttcgat gtgactcact ccaatgttcg
     2941 tataccggat gataaaacat atcagggaca aagttatcgt gtttcctgga acaagttatt
     3001 cgaagaaaca agtacttcac tgaatatcgc ggcctatcgc tattcgacac agaattacct
     3061 tggtcttaat gatgcactaa ctctaattga tgaagtgaaa catcccgaac aagatcttga
     3121 accgaaatcc atgcgtaatt actcgcgcat gaaaaatcag gttacggtca gtattaacca
     3181 accgctaaaa tttgagaaaa aagattacgg ttcattttat ctttccggaa gttggtccga
     3241 ttactgggct tccggacaaa atcgtagcaa ttactctatt ggctacagta acagtgcatc
     3301 ctggggcagc tacagtgtca gtgcccagcg ttcatggaat gaagacggcg acactgacga
     3361 tagcgtttat ctcagtttca ccattcctat tgaaaaatta cttggcactg aacaacggac
     3421 ttcaggtttc cagagtattg atactcaaat gagcagtgac tttaagggta ataaccaact
     3481 caacgtcagt agcagtggct atagtgataa cgctcgcgtc agttatagcg taaatactgg
     3541 ctatacgatg aataaagcca gcaaagattt gagttatgtt gggggttatg ccagctatga
     3601 atcaccgtgg ggaaccctgg caggttcagt ttctgcaaat agcgataaca gtcgtcaagt
     3661 gtctctcagc accgacggtg gttttgtatt gcatagcggc ggcctgacct tcagtaatga
     3721 cagttttagc gactccgata cactggcggt agttcaggct ccaggtgctc aaggagcgcg
     3781 aataaattat ggcaacagta ctatcgatcg atggggttat ggtgtcacca gcgctctttc
     3841 tccttatcat gaaaaccgta tcgcgctgga tatcaacgat cttgagaacg atgttgaatt
     3901 aaaaagtacc agtgcagtag ctgtaccgcg tcagggttca gtcgtctttg ctgattttga
     3961 aaccgtgcaa gggcaatcag ccattatgaa catcacacga agtgatggta aaaatattcc
     4021 atttgctgca gatatttatg atgagcaagg caatgtcatt ggtaatgttg gacagggtgg
     4081 acaagcattt gttcgtggta ttgagcagca gggaaatatc agcattaaat ggctcgaaga
     4141 aagtaaaccc gtaagttgtc ttgcgcatta tcaacaaagt tcagaagcag aaaaaatagc
     4201 gcaatctatt attctgaatg gaattcggtg tcagattcag taactacaag ggacgtcaaa
     4261 tgataaaaat aacgccacat aaaatagcga tactgatggg aatattatta tcaccatcag
     4321 tatttgcaac ggatattaat gtagagttta cagccactgt caaagcgaca acctgtaaca
     4381 tcacacttac tggtaataac gtcacgaatg atggcaataa taactacaca ttgagaatcc
     4441 ctaagatggg tctggataag atcgcgaata aaacgacaga atctcaggct gattttaaac
     4501 tggttgctag tgggtgcagc agtggcatca gttggattga taccactctg agcggaaatg
     4561 catcatcaag ctcacctaag cttattatac cgcagtctgg tgattcatct tcgacgacaa
     4621 gtaatatcgg tatgggtttc aaaaaacgga ctactgatga tgccactttc cttaaaccta
     4681 acagtgcgga aaagatacgc tggagcacag acgagatgca gcccgataag ggtcttgaaa
     4741 tgaccgttgc gctacgtgaa acagatgcag ggcaaggcgt accggggaat tttcgtgcac
     4801 tggcaacgtt taatttcatc tatcaataag aaggataatt aagatgacat ttaaaaattt
     4861 acgttatgga ttatccagca gtgtcgtttt ggctgcctca ttgttcagcg taccctctta
     4921 tgcggcaaca gacagcattg ggctgaccgt gattactacc gtagaaatag gtacttgtac
     4981 cgctacatta gtaaatgact ctgatcagga catttctgtt gttgactttg gcgatgtata
     5041 tatttctgaa atcaatgcca agaccaaagt taaaacattc aaactcaaat tcaaagactg
     5101 tgcgggtatc cccaataaaa aagcacaaat aaaattaacc aagcgagcca catgcgaagg
     5161 tactgctaat gacggtgcgg ggttcgcaaa tggatccaca gccgcagata aagcaagtgc
     5221 cgtcgccgtt gaagtctgga gcaccgaaac tccggcaaca gggagcgcaa cacaatttag
     5281 ttgtgtgaca ccagcatctc aagaggtcac tatctccaat gcagccaacg cggttgttta
     5341 ttatccgatg agtgctcgac tggtcgtcga aaaaaataaa accgtaagca atgtcactgc
     5401 gggtaaattt tctgcacctg ccacatttac agtaacctat aactaacaga ctgacagatc
     5461 caggaactat atatgcatcc cactcaacgt aagctgatga agagaataat tctgtttctg
     5521 tcactacagt ttcccattac ctatccagcc atcgctgggc aggatattga ccttgttgcc
     5581 aatgtaaaaa acagcacttg caaaagcgga atcagtaacc agggtaatat tgatcttggc
     5641 gtcgttgggg tgggatattt ttcagataat gttactcctg aaagctatca accaggtgga
     5701 aaagagttca ctgtcacagt atcagactgt gcattacagg gaactggcga tgtgcttaat
     5761 cagttacata ttgattttag agcccttagc ggtgtcatgg ctgcaggttc aaggcaaata
     5821 tttgccaatg aagtcgcaac cggggctaaa aatgtcggtg tcgttctctt ttccattcag
     5881 gacccgacca atatattcaa tgttattagt tcagcaggaa attctcgttc cgtgtatcca
     5941 gtaatgactt ctgccctgca taattcttca tggaaatttt atgccagaat gcaaaaaatc
     6001 gatcccgcgc tggatgtcat atctggccag gtaatgagtc atatattagt tgatgtgtac
     6061 tacgaataat agatcaaaac aaataactgc gactgttaca gcagtagatg gtggagtgat
     6121 aatgaagaaa ttcttcagac attttctttt tttaattctg tgcttgagtt gttataccgc
     6181 gagtgctggc actgatgata acgtaggata tattgttggg aatagttacg gcgttggtcc
     6241 gtcagatcaa aaatggagag aaacggggcc gaatggcgat gctactgtca tattcagata
     6301 tgcaacaagt acaaataatt tagttttcta caaaccgacg cagcttggac ctacaggtgt
     6361 aaaattacag tggagtcagt tagataccgc ttctggtggt ggttttcttt attgcaacag
     6421 aagtgatagc acaagtggta gcgcaatgcg tattgaaaat gcaatggttg actcaggtaa
     6481 aatgtatggc tcccataaat tatttaatac atcagttcct ggtttgtatt acacattatt
     6541 aatttcaaac atgtggtcag cttacggtac cgtaactaac gttagttcac ctgggatata
     6601 cattggtgac tctgcagaac aatatttttc gtggtataat ccaagcgaag acgtgttata
     6661 ttagagttgc aataatgcga atagcacccg taaatactgg gctgtaggtg gtatttatca
     6721 gacccttaca attgaattct atacagatac aaactttgat ccaacagtaa ctcaacagat
     6781 caaattatcc agctcatcaa attatctgta ttcatttaaa gcctatggcg caggccaagg
     6841 tataaatgag catagttatt ttatcaaaat cgatttcgat ctgctcaatg tcaagttgac
     6901 taatcccact tgttttaccg ctatgcttag tggaacttct gtgactggtt ctacggtaaa
     6961 aatgggtgaa tatagtgcag aacaaatcag aaacggtgcc acaccggttc cttttgatat
     7021 ttcacttcaa aactgcgttc gtgtgactaa tattgagaca aaattagttt caacaaaggt
     7081 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgacg cagcaaaagg
     7141 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
     7201 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
     7261 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
     7321 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
     7381 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgca gtaattgata
     7441 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
     7501 gatacgttat gaaaccaacc accattgctt cacttcagaa atgtaagcag gataaaaagc
     7561 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaaggac
     7621 ttaacgtcat gctggtaggc gactcgctgg gcatgacggt tcagggtcac gactccaccc
     7681 tgccggtgac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcgccaa
     7741 actgcctgct gctggctgat ctgccgttta tggcgtatgc cacgccggaa caagcctttg
     7801 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
     7861 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
     7921 gtttaacccc acagtcagtg aatattttcg gtggctacaa agttcagggg cgcggcgatg
     7981 aagcgggcga tcaactgctc agcgatgcat tagccttaga agccgctggg gcacagctgc
     8041 tggtgctgga atgcgtgccg gttgaactgg caaaacgtat taccgatgca ctggcgatcc
     8101 cggttattgg cattggcgca ggcaacgtca ctgacgggca gatcctcgtg atgcacgacg
     8161 ccttcggcat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     8221 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     8281 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     8341 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     8401 tatgggtaat ctgcacgatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     8461 tgtggtcgtc gtcagtattt tcgttaaccc aatgcagttt gaccgcccgg aagatctggc
     8521 tcgttatcca cggaccttgc aagaggactg cgaaaagcta aacaaacgta aagtggattt
     8581 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     8641 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     8701 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     8761 tgaaaaggac tttcagcaac tggcgctaat ccgcaaaatg gttgccgata tgggcttcga
     8821 tattgaaatt gtcggcgtcc cgattatacg cgccaaagat ggccttgcgc tcagttcccg
     8881 taatggttat ctgacggcgg aacaacgcaa aattgcgccc ggtctgtaca aagttttaag
     8941 ttcaattgcc gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     9001 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     9061 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     9121 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     9181 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     9241 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     9301 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     9361 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     9421 atagactccc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     9481 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     9541 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     9601 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     9661 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgtgc cagctccggc
     9721 gagtaaaata tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     9781 aacagtatcg gcaccaccag cggcagctta tcgtgatccg cctccagatg ccggtgcatg
     9841 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     9901 tcaatcacaa catgcagata accgggattg ccctgcattt gcacggaata gagcacgtcc
     9961 gtgctgtgtc ctttcaggct ctcttcaatg aaactccccg actctaaatg aagcgtgttg
    10021 aggtcacaaa gttctcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
    10081 tccgcgtgca ttaaaaattg tttaaacact gcatcatgcg gtgtggtact cggtgcatcc
    10141 atcttccctg ccttccgtgt tgtcttgcgt gaaggcagta tccagtcaag gcgtgccatc
    10201 gtcagccacc tctttagtgt ttaccgagca gcgttcagag aaatttcaat tggttacagc
    10261 cgttacatgg cacgcttcgg cacggtacgc aaactgctga tgtaaatgcc gtcagatgag
    10321 tagacactaa acaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcatcgcc
    10381 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
    10441 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
    10501 accaggattt tcttgacgca gccggtattc tcgaaaacga agccattgat atctggaatg
    10561 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
    10621 tttctgttaa cggtgcggcg gcccactgcg ccagtgtcgg cgatattgtc atcatcgcca
    10681 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaacgtc gcctattttg
    10741 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
    10801 tatccctgcg gctggttact caccagccgc gacatcgtct ccagcgaatc cgttcttaag
    10861 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
    10921 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaatccg
    10981 ccaaacggat acgaaagata ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa
    11041 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgatag
    11101 ccatccaccc gatgcaaaaa atgggtatgt gactgaaaat caaaaacatc gcgaatttcg
    11161 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
    11221 ttgatgcgcg aggtaacaac aaacgccgtc gccttcatgc catattgttt caatacagga
    11281 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
    11341 gggagattga tcttattctt cacgtaacct tccagctgcg ccatgctcag tgtcgcgtat
    11401 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
    11461 gtatggcgaa aacgggtgtt ttcttcatcg cgcagaatat ggtgataggt tagcaccggc
    11521 aggccattat cgggttgggc atccagcgcg ctgatatagg cgagtcgatc gccaatacgg
    11581 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
    11641 ttgtccgcca gtaccccaaa tggcgcactg cccgcactcg gcgcgttata gaccggcgta
    11701 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
    11761 tcaacttttt gtcgcccctg aactggctcg agatgccctt tatcgataaa ccctttacca
    11821 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgcccg
    11881 gcccgaatat tccccacggt caccatcttg tcgccaattt gcgcccagat cgcagcattt
    11941 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
    12001 agcaggagaa taacagcttg tttgtacatg aaaaaataac cgagggtgaa acaacgcttt
    12061 tattgtagca aaagcgctat aagaatcgtg ggttaatact catacaaatt atgtttgagg
    12121 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
    12181 tcgacaatac gctcgcgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
    12241 tcaattaacg gtaacgtgac accagaaatc acttcacaac gggagtgttt atggcttaat
    12301 aatgaagcca cgcggtacgg tggcgctccg gctaaatcgg ttaagaagat gacaccctca
    12361 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
    12421 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
    12481 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
    12541 ctccttagcc gaaaatcagt ctaccctgac cattgttgat aaagggtgat agccgtcaaa
    12601 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
    12661 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
    12721 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
    12781 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcaggg
    12841 agtaaaacac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
    12901 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
    12961 ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
    13021 acagtgaaat tgccgtcacc agaatgccaa caaacagacc acgcgccacg ccaccgccga
    13081 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
    13141 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcattg gtgatcaccg
    13201 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
    13261 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
    13321 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
    13381 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
    13441 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
    13501 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgcagcactt caacttccag
    13561 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
    13621 tgcgagatcg agaataaagg tttccgattt cagcttagcc agcagcgcct tcatcgaggt
    13681 attttccacc agctcaccgt gttgaataat gccgatattg cggcacagca tttctgcttc
    13741 ttccaggtag tgtgtggtga gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
    13801 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
    13861 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
    13921 taacatacgc gcacgttcgt tgcgttttcc ccatagatcg agctgcttaa gatatttttc
    13981 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
    14041 ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
    14101 tttagcgttc acgacatcct tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
    14161 tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc
    14221 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct atcccacgaa gcgcctgaac
    14281 gccgcctgga taggtttttt taagctgttg aagttccagt gcaatggtca taaattttta
    14341 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
    14401 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
    14461 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
    14521 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
    14581 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
    14641 aacctggtca ttcacactga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
    14701 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgca
    14761 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
    14821 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
    14881 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
    14941 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
    15001 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
    15061 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
    15121 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgccg atatacggca
    15181 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
    15241 agaaaccgat aaattctacc gggacgttca cttcacgacg ggacggttta tccagcagcg
    15301 tacaaatcgc cagcgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
    15361 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
    15421 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
    15481 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
    15541 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
    15601 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
    15661 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
    15721 gcaggttttg gcaccgcaac aaagcgcaag acacgcagta taccagtaaa actattcatt
    15781 cgcggaatct gttaataaag ctcgaattgt gatgacgatc acacatgtta aaacctataa
    15841 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
    15901 caggctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
    15961 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
    16021 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgt gccgcgcttt
    16081 ggctatacgc cgccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
    16141 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
    16201 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
    16261 tggcactgct gattagcggt agtatcctga cctgggccgg atttaacgat ccgcaggaaa
    16321 tcaacggcac cttaagcgcc gatgccacac ctgcggaagc tatctcaccc gttgccgatc
    16381 aggactggcc tgcctatggt cgcaatcagg aaggtcaacg cttttcacct ctgaaacaaa
    16441 ttaacgccga taacgtccat aatctgaaag aagcctgggt gttccgtact ggcgatgtga
    16501 agcagccgaa cgatccgggc gaaatcacca atgaagtgac gccgattaaa gtgggcgaca
    16561 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct cgatgccgcc agcggcaaag
    16621 agaaatggca ttacgatcct gagctgaaaa ctaacgagtc tttccagcac gtaacctgcc
    16681 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
    16741 gcccgcgtcg tatcattctt ccggtcaatg atggtcgact gattgcgatt aacgctgaaa
    16801 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
    16861 cagacaccaa accgggtctg tatgaaccga cttcgccacc gattatcacc gataaaacca
    16921 tcgtgatggc aggttcagtt accgataact tctcaacccg cgaaacgtct ggcgtgatcc
    16981 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatcccggc gcgaaagatc
    17041 cgaacgcaat cccgtctgac gaacacacct ttacctttaa ctcgccaaac tcgtgggcgc
    17101 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    17161 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    17221 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    17281 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    17341 cagttattta cgctccggcg aaaaccggca acatttttgt gctcgatcgt cgcaatggcg
    17401 aactggtggt tccggccccg gaaaaaccgg tgccgcaagg tgctgcgaaa ggcgattacg
    17461 ttactccgac ccagccgttc tctgaactga gcttccgtcc gaagaaagat ttgagcggtg
    17521 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    17581 tgcgctatga aggcattttc acgccgccat ctgaacaggg cacgctggtc ttcccgggta
    17641 acctgggaat gtttgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    17701 ccaacccaat ggcactgccg tttgtttcga aactgatccc acgcggtccg ggtaacccga
    17761 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    17821 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc gtttggtctg ccgtgtaaac
    17881 agccagcatg gggttatatc tcggcgctgg atctgaaaac caatgaagtg gtgtggaaga
    17941 aacgtattgg tacgccgcaa gacagtatgc cgttcccgat gcctgtaccg gtgccgttca
    18001 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    18061 ctgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    18121 agggccgttt accagcgggt ggtcaggcga cgccaatgac ctatgaagtg aatggcaagc
    18181 agtatgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    18241 ttgtggctta tgcgctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    18301 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    18361 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    18421 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    18481 agccgctggc ggtttgccat tttctgacaa gatacggaac tgggtgccgt gaatatggaa
    18541 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    18601 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    18661 gaaatcgaac ttcccgccat ggttcatatg attcatattg ccgtgcccca tatggcccat
    18721 catctggcta tgatccatcc cggccatcgc ctgatcgcca tatttctcca tcagcatctg
    18781 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    18841 ttccagcgaa ggtaacgcag gcaggctact taatgtgtct ggcaaagcac cggaggcact
    18901 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggcg caatcgccat
    18961 ccccatctgg ctgaccggta gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    19021 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    19081 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    19141 gagcgagcgg gcattacagc cattaagcaa acgcaggcgc agccaaccac gcggggcagc
    19201 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    19261 ggtcatcaca tccagttgat aatcaatctg cccatcagcg ttaaattttt tatcctgaac
    19321 aatcaccgga acatcatcga tgccccactg ttttggcagc attaatttca ggatctcgtc
    19381 atcttcaatt accaccagcc cagccaaccc catcgccacc tgtcgcccgg tcttgccatg
    19441 ttgatgtgga tggaaccagc aagtagcggc aggttgatca acgttcaacg tcaccgagcg
    19501 cttgccacct ggcagaataa ttccctgcgg gccgccgtcg acttcacccg gtacttccag
    19561 cccgtgccag tgcaacgtcg tctcttccgt cagttggtta tagatatcaa ccgttaccgc
    19621 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    19681 tgcagttttc tcgccaaagg tagactgacc tgcgccaata gttaactgaa tgcgattacg
    19741 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    19801 gcggctccac agcggcaagg ctgaagccac acccagcgcg acggaatatt ttaagaaatc
    19861 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    19921 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    19981 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    20041 aagacgtttt tcagaacagt gttattcggc agcctgatgg ccgtttgcgc aaacagttac
    20101 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    20161 aagaacgatt gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc actggtcttt
    20221 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagccctc
    20281 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaagtgcaaa
    20341 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    20401 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    20461 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    20521 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    20581 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    20641 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    20701 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    20761 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    20821 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    20881 gatccgcgct ttaagctggt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    20941 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagtctgttc
    21001 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    21061 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    21121 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    21181 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    21241 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    21301 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    21361 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    21421 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    21481 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    21541 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    21601 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    21661 caccccggcc cactgccaga aacggtcgtt gcccatctcg ataaaagtca tatttgcgta
    21721 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    21781 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    21841 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttacccgcga cattaacggt
    21901 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    21961 aaggcgctgt atgacatggt ggatgtgaac gtctatcagg aaaatatctt ccataccaag
    22021 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    22081 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    22141 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    22201 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    22261 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    22321 cagcgaattc cagttgattg gcgcgaacca tcacctcttc accgtccatc cacagggtgt
    22381 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    22441 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    22501 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    22561 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcattgc gccattgatg
    22621 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatggtttag aaataaaaaa
    22681 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    22741 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    22801 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    22861 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc aattctgcag
    22921 aagccaggaa gacattcgcg ccagtcccca gacggttcgg gaagttacgg gtagaggtgg
    22981 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagccag
    23041 ggatctcgat acgcgcaccg ctcttaccga agacgctata gtagccttct tcggtcaact
    23101 gcgcggcatc catacgggtt ggcggtgcca cccacaggcg cgtcggcaac tggcctttgt
    23161 gcgcatccag cagtttaccc gcagcacgga agtgaccgat gttggtcatg caggaaccga
    23221 taaacacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcgtccgggt
    23281 cgttcggtgc acagaggatt ggctctttaa tatccgccag atcgatgtcg atcactgccg
    23341 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    23401 cctgaatacg acgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    23461 gcaggacgat gttagagttc aggtattcga tgatcggttc tttgttcagc ttgatggtac
    23521 aaccagcggc agaacgctcg gcggacgcat cggtcagttc aaatgcctgc tcaactttca
    23581 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    23641 tctcaacggt cagcagacct tgtttgatcg catacagcgg gatcgcgtgt accagatcgc
    23701 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    23761 ccagcggcat tacgcctgtt gcggctgcaa atgccaccag accagaaccc gccgggaaag
    23821 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    23881 gcatacggtt cagccaggag tggattacgc cgtcacccgg acgcagcgac acaccaccac
    23941 ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg
    24001 ccgcggtatg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    24061 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg tgaacctaca gaagtcattt
    24121 tcggttcgca gtacgcgccc ggacgaatac ctttcacacc gcaggcacga cctaccattt
    24181 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    24241 cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca
    24301 gcgggatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    24361 tcgccagcag ttcgccggtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    24421 tcacgtcgcc catgttcagg ttagagacgt cgacttcgat tggcagtgca cccgcgtctt
    24481 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    24541 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt
    24601 tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt
    24661 gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt
    24721 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgc gaccacgcat
    24781 ccggtgccgg agaaaggtca tcggtgttag tttcgccagt gactttgaag acagtaacgg
    24841 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    24901 taacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    24961 cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat
    25021 ccagcgcgtc gatcagcgaa tgaatgttgt aaccaccctg catggtgccc agcagttcga
    25081 tggctttttc cggagtcagc agaggggatt tggcttcgcc tttcgcgata gcagccagga
    25141 agcctgcttt gacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    25201 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agtgcggcca
    25261 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    25321 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    25381 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    25441 cagaatagca ggattttgac gatgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    25501 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg
    25561 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    25621 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    25681 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    25741 cagtgccggg ctttgtgccg cctctaccgg gctatatata aaaagccgca cagtggaagc
    25801 gcctgtggaa ccgcaatcga cacaacagac tgcgcctgac atcaccgcag ttacgcttcc
    25861 tgcaacggtt tccgcaccgc ccgtaacgcc cgccgtcgtc aaatccgcat tcagcactgc
    25921 acaaatcgat caatgggttg cgcctgtcgc gctgtacccc gattctctgc tttcacaagt
    25981 gttaatggca tcaacctatc cggcaaacgt tgctcaagca gtgcaatggt cgcacgataa
    26041 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag
    26101 cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg
    26161 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca
    26221 gcgattgcgg caactggcgc aacaaaccgg ttcgctgaag tcatcaaccg aacaaaaagt
    26281 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc
    26341 atccaatacc gttttaactg ccagccccgt cattacagag cctgcaacaa ccgtcatttc
    26401 cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    26461 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc
    26521 gtttattgac agctttgtgc gcggattcgg ctatagcatg ggtgttgcta ccacgtacgc
    26581 actattcagc agcatcgact gggatgacga cgatcatgac catcatcatc atgacgatga
    26641 tgattatcat caccacgatg gcggtcatcg tgacggtaat ggctggcaac acaacggcga
    26701 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa
    26761 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    26821 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagcgcca cgcagctacc
    26881 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    26941 cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    27001 agctgaaaac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    27061 ccagcaacaa aaggacgccg ctcgtcagcg ttatcagtcg gcctcgcctg agcagcgcca
    27121 ggcagtccgc gagaaaatgc agactaaccc acagatccag cagcgaagag actgtaacca
    27181 ggtgaatcat ttgataccaa cgagggtttt agtgaaagga ggtgaaatca cctgtaagat
    27241 actgaaataa aacaggtgat gtctggatga gcgcttaaag tctatttgat accgattgtg
    27301 caataattaa tcgtttattc taaaatcggc aaaacttttg atactgcgat ctcaactttt
    27361 taaagcgttt aaaacttcat taaatctata tggcaactaa catacgggcc agcctcgcca
    27421 aactaacccg ctttagattc taccggattt gattgcctac ttgtctcggt tttttccaaa
    27481 atctctctga cgcaatcgtc tggcatatct acaatcatca acgcagccag gcgcttcctg
    27541 ccttctagtt gtaataaatc ctggttttct ttatccagca gtgtcagaac cgtctctaca
    27601 cctttgcgag caaggagatt ggttaactgt tctcgttcgt ctttcgtaag gaatgaaagc
    27661 atctgtgtaa gttgatctcc cttatcacca gaacaaccaa aattaccaca aataccaaca
    27721 tcgtttggtg attcaccctt gccctcttta agccactcaa gactaactcc ttcactttca
    27781 gaaattcgta taaggatact ttctctgggg gctggttgtg tttgtcctcc cttgaaatag
    27841 ctgtttaatg tgttgatatt gattccccac gctctggcag ctgcacttct tgaagggtag
    27901 cgctttgtaa gcattccaat acgttcaata acaggttctt ttccctgcga cataaagata
    27961 cctcttggtg cttttctaag gaaaggtttc tttctattta tccaattgaa aaataaggat
    28021 aaaagcaaaa attagccaaa ataccaaaaa taccttgatt tggtattttt agtgatgcat
    28081 tatttacccg tgcagatatc cgcacgaata tccgcgcggg taacttttta gaatagagga
    28141 agtatgagta gaagagaagt ttcagatcac gattggccgc ctgaaatcat caaggcgcgc
    28201 ttacacatgg ctggcttatc actgcgctct ttatctttaa aggctggtta cagcagggat
    28261 tcgctgaaaa gcgtattacg tactccttgc cgaccgtatc agcaaataat tgctgatgct
    28321 cttggggtat cgcctgaaga aatctggccc agtagatacc aggttaagag ctatatgaga
    28381 aaggcgtcat gatatgtatg tcattgcaaa agaactgatt ggcgcgcccg gaatgcctgc
    28441 tacaacaaaa ggtattcgcc aggcattaca acgttacgta caagggaaaa gctgttgttc
    28501 ccgtcgtcgc tcaggctcta aagcaactga atacagcatc gactgtttac ctgaagtgac
    28561 gcagcaggca ttacgtgaac gttatgccct gcaactgatg acgcaaaaag ccgatgaatc
    28621 accggctccg gtagtgacaa aggccaggcg ctcacctgcc gtggttgatg cggtggaggc
    28681 atatcgcgga tcaccacaac tgatggtcga acgccttaat gccctgactg aaaaccagcg
    28741 ccaggtggct gatgcacgaa tcgcgatcgt cagcgaagtt ctgaaagtcg cgcaacaacc
    28801 tggtttcagc tgtgcgaagg ctatccggtt tatcgttgac aacctggcac gttcgcagct
    28861 ggacgagcgc attgtggcaa tggttgagac ggcgaacgcc aaaaagggaa acagccgcgc
    28921 gttaagtgaa atcacgctga aacgctggat tgcggccttt aacaaggcgc agaacgccgc
    28981 tgaacgcctg cttttactgg caccgggtaa gcgccaggaa ataaaagccg aagatattaa
    29041 ctggctgccc gaatttctgg cgcagtatcg ccagtcaaac ggccgaccaa tgaccgaggc
    29101 ttacgaggat tttgtcgctg aatggcagca ccggcacgct gatgagcctt atatgctcga
    29161 tatcatgccg tcttatgaca ccattcgccg cgcaatgaag aaactgccgg aagtggtgaa
    29221 acaaaaaggc cgggtgaccg gcagtgaata ccgccagctt gagggattca cgcgccgcga
    29281 ctggtcaaaa atgccggtga attatgtctg gattggtgac ggtcacggca tgaagctgaa
    29341 atgcgcacac ccggttcacg gtcggccatt tgcaccggaa gtgacctttg ttatcgacgg
    29401 tggcacgcgc tttgtggtgg gctggagcct tgacctggct gaaaatgttt tcgccgtagc
    29461 cggtgccata cagcacggca ttcgccatca cggcaaaccg tttctgtatt actcggataa
    29521 cggctccggg gaaaccgccg acatcctgga taaggaggtt gtggggatac tgccgcgact
    29581 ggggattaat cacccgaccg ggattgccgg taatccgcag ggacggggca ttatcgaacg
    29641 gcttaaccgc acattaccga tgcgcatagc ccgtaaatac cgcacctatt tcgggaaagg
    29701 tgcagatcgc gagacgttac gcaaaaccaa ccgcgattta cgctcggcat ttactgccct
    29761 gcaacagggc aaacggctga acgcccggca gcagtcagcg atgcgtgatt taccgtcctg
    29821 gtctgaactg attgatgcca ttcgtgatgg tgttgagtgg tacaacaacc ggccgcacga
    29881 tgaattaccg gtgaagccga acggcaagca ttacagcccg gcggagttca gaaaaaaacg
    29941 cctggcggaa gaggacacgg aaattgaatg gctgtccgat gtggaattgc gggacatgtt
    30001 ccggccgatg gtggaacgcc ctgtaagacg ctgtgaaata cgctggctga ataatattta
    30061 ctacgcgccc gagctgcgtg atgagcatgg ccgcaaggtg cttatcagct atgacattca
    30121 tgatgccgaa cgaattaccg tacgtcgcct ggatggcagc gtgatttgcg aggcggtatg
    30181 ggacggtaat aaacgcgaag ccttcccggt tagcgcggaa tactacaaac agcagcaacg
    30241 ccttaaaggc atgcgtaaac gcgcagagga aaaaatccgt gatgccgagg atgaggttgt
    30301 caacgtgctg gagcacaagc cgcaggagcc ctggctggaa aacatctatc gcccggtagg
    30361 taatacggtg gccgttcagc aacctgccgt tgatgatgag cctgatgaag aatacgagcg
    30421 caatttccag cggggattgc agttgctcga agcgaaatta aaagaaaatg acccgctggc
    30481 ctgaaataaa aaataacccg agcggcgact caggttattt gattaaacaa ggtatcaaat
    30541 gagaggttaa taatatgact gatattaacg atgtaatcaa gaccattgat gaacttattg
    30601 atggcggcgt actgacgcag tatgccatcg ccagagaggc gggaatttcc gacggcacat
    30661 tatcggcttt ccgcaagggg aaatataaag gcgataacgc cgctgtggct gcttccctgc
    30721 gttcctggta tgagaactgg aataaacaaa gcgcactgcc ggaaccgccg cagtttgtgg
    30781 aaacgcagac agttcaggag ctgcgcgcac tgtttcaggc ggttcgtctg atgggctgta
    30841 ttaacgttat tgtgggcgta ccgggtgtgg gtaaaacggc cactgcccgt aattactgcc
    30901 aggagcaacc aaacacctgg atgatcaccc tgtcacccgc gcactccagc gtcacggagt
    30961 gtctgctgga gctggccgat gcgctgggga ttgattacac ccgcgcgaac aaaggggcat
    31021 tatcccgcgc catccgccgt cgcctgatgg gaacgcgtgg actggtgatt gtggatgagg
    31081 cggatcatct gggtattgac ggtctggagc aactccgggc aattcaggac gccacgggga
    31141 tcgggatggt gcttattggt aacccgcgcg gattgtttaa aggtggacgc cgcgcctttg
    31201 atgatttatc gcgcctgttc agccgtcttg cccgtacaaa acaacttcgc aaggccaaaa
    31261 aggcggatgt gctggccatt gccagggcgt gggggatcag tggtgaggcc gagctggccg
    31321 tcatgcaggc tatcgctgaa aagccgggag cgttacgcgt tctgacacat acgcttaacc
    31381 aggcgtggct caccgccagc ggtgaaggcg cggcgctgac agaaaaacat attaatgcgg
    31441 cctttaaaga ggtttatacc aaccctgaat tactctcaca ggtgtgatta tggcggtatt
    31501 taatattcct gatatttacg gacgctttta cctggttaat ttcgataacg tgaaggtgat
    31561 ttcactggcc gaaaataaag aatgtggcga tttacttttt gaatttaatg accgcacacg
    31621 aatggtgata tctgccggac ttgatcgcga aggtgcgaca gacgtttaca gcggaatatg
    31681 ccgttctgtt ggtgcgaaac aagtcagcta aatgaggtgt tatatgaata ttcaatcctg
    31741 cggtaacaaa atgaatttat tcgactccct gaacagcgcg cgccgtctga ccgaacttgc
    31801 cggtgcggta ctggaacgca gtaagcgcta tccgcaacgt tttgcactga aaaccacgcc
    31861 gccggtaggc aacgtgcagg gaaccggtga aattgaaatc accatacaga ccaacggcct
    31921 gcgtcgccgt gtgaaggcca cccgcatcag cggctgcacg gtttactggg aggtatgagg
    31981 tgaaaaaaaa tctcattgca tgggcgtggt cgagtggtct tattgagttt ggttacgtcc
    32041 tgccggaagg tgcattaccg atagttgccg gaaagcctgc cacggtacgg catgtgattg
    32101 aggttatggc gcgtcatgga cgtgatgaac aggagcagtt actggttccg gggataccgg
    32161 aagcggtgac ggaggaagaa gcctttaatg ccatgattcg gttctgccgt gaggtcagac
    32221 gccgggtcag ttatccaaac agaacgagga ccagaggatg agtaaagttg tacgcattat
    32281 tttcgaatac aaggagcacg ttatccataa aaacgcggat ggaacagtgc gcatgggggt
    32341 aagtctggac atacgttcaa ccgggataaa gcagaaaggt gatggacctg ccatgatttt
    32401 tggggtggtt atgctcgcgg aaagcagaga ctttactgaa cttgtggcaa tgaaagccag
    32461 tgcgctcatg aaagatatgg acatgagttc cggggttatt aaaggtaatg aatttaatca
    32521 gcaggggtaa ttccatgagc aaagtacgcg ttatttttga atttgaccac gtaatgcatg
    32581 aagtaaagcc agccggtaat gacagtgagg aaatcacgga gggggttact gccacggtaa
    32641 aaatcgaacg tgatacagag aacagaccgg caggaccctg tgatgtttat gcacaaattc
    32701 ttaaatatca cagccccaca atcattcagt ttctgacaga tgaactacag ggttccatgc
    32761 aggccatggg ggtaagcagc tccgttgaac gccgttctgt tcagaacgga ccagatacat
    32821 tgcaataagg aaaaacaaaa tggcaaaacg cgttacaaaa ttaaaggccg cagcagaggc
    32881 ggcaccgcag acccgcgaag aagtcagccg cgatatccgc accctgggcg atattcagcg
    32941 agaggcgctg cgcctggaaa cagcgatgaa tgacgaggtg gcagaaatca ccgcccgtta
    33001 tacgccgcag attgaaaacc ttaaaaaaga aatcaaagtg ctttttaagg ggattcagga
    33061 ctggtgcaaa accaaccgcg atgagctgac gaacggcggc aaaacaaaaa ctgccaatct
    33121 gaccaccgga acggtgtcat ggcggctggg aacgccatca tgcagcgtca gccgtgatgt
    33181 ggaaggtgtg attgaaatgc tgcgccgtat gggtcttgag cgattcatcc gcacgaaaga
    33241 ggaagtgaac aaggaagccg tcctggcgga gccggatgcg gtgaaaggga ttgccggtat
    33301 taaggtaaat aaaggcgctg aaagttttta tgtcgagcct tttgaacagg acgccggact
    33361 gaacaaataa catcgcatta aatctttaaa tatcacatcg ttttaagtat ggcgctcgcg
    33421 tcaggggact gcctgcgcct gtaaactgaa aataaggaac aggatatatg gcatattttt
    33481 atttcaaatt agaccgtgtg cagacaaata aatatttcac caaatatcaa cagactgttt
    33541 taccgctacg caacagtatt cttcgggcat tactgaaaaa tacaggcgct gccggattgc
    33601 gcttaaagcc gtttgccatg gacgtaatca gtgagtttta tttttctggt gctctgcctg
    33661 cgggctggcg taagcgcgat gatgtggctt ttatcgggga cggaccgtgc tttattgcca
    33721 gacctgatga gtcatgccct gaaggaccgg cgattgccgc aatgattgaa gccgctgaac
    33781 gcgagttaag aaagcgtcct gatttccttg tctggctctg tgaaaagctg ggggtaatga
    33841 gaatcccctc catgtttaac atggactcct ggtggacccc gtcgctctcc cgtgatgccc
    33901 tgtgcgtggt gtttaaagta ggcgcttatg gcagggaaat aaaagggggt attcctgaag
    33961 aatgccagga gattaaacat tctgaatatg tggcgctgac ggaggaataa ttcatgattg
    34021 atgcaaaagt gcttgaaggg gttaaaaact ggctgagtat ttacgggcgt ctgacctgcg
    34081 gcattctggc tgaaaaaatg aatatgccgc catcctcgat ggtttatttt ctgcgtgatg
    34141 cggtcgatgc cggggtgctg acggaatgta acggttttta tgatattccg cgtccccgcc
    34201 cggtgcagcc ggttcgtcgc aaatgcagcc aggaaggtgc ggctgatgat gttcagtggt
    34261 gcagtttcag aaaatccctg ccgtggattg aggggcatga tattccgtca atggcgtggg
    34321 aatttgctca gggcgttctg acctgtgaaa ccgtttatgt ggtggctgaa gttgatgagc
    34381 aggccatgaa agaaggcgtg ccccagtttg tgatggcgta tatcgacata cgcctgggtg
    34441 tcattatctg cggtttaagc ggctggaata tcacctcgcg cgttctgcgt tacctgattg
    34501 ttgaccggac ggccgcgcct gctgggatat ctgcggaggt ggcgtaatgt tctttaaaac
    34561 atcaaaccct tccgcgctgg ccgcgtggca aaaatatcag caggactgcc agaaagttaa
    34621 ggatgaggca aaacgccttg aggccgtgct gaatgttgcg tgccggtcgg tatttgtatc
    34681 cggtatcagt ggcttttgtt ttaaagggct gcgctttatg gatgacaaat atccttttca
    34741 tcgcgactta tggcgaaaac cgactgcgtc gaatggctgg agctgcacac cgcgcacatc
    34801 acgtattccc aaagccctgc gcgttgcctc tgacgaactt aacagtctgt ggcgtgaata
    34861 ttcgcccgtc acgtatgcca gaaccgatgc actgttgttc tggctgggta ttgatttctc
    34921 ggcaatcatg tttggccctg tgaagtggtt ctgcgttgac gatgtgattt accttgagtg
    34981 cggcgtaaaa cccgcaaatc agaaaatgac cgaaattctg tctgatgagt tttatgctgc
    35041 tgaaaagcga gtcagggggt gatgtatgat gattttacaa cccatggggc gaaaaggtca
    35101 ggcacctgct catgtccgtg cgtggacacc tgaagaagat gcgctgctga ttgcgcttta
    35161 tccatccacc ccggttaagg atattgctgt cagggtaaaa agaagtttct ggggtgtaca
    35221 taaccggatt gttttattac gcggtactta cccggagttg ctcaaatgca aacgtccaag
    35281 atttaaacat gatgaagata aatttatccg aaaaaatgcc agaacgatga ccggtaagca
    35341 aatcggagaa tatctggggc gagaccggga ttctgtccac aatcgggcgc gatatattgg
    35401 cgtaagcatg aaaaaatacg gagaattgct gcccttcacc cgtatacctg acgatgatgt
    35461 tcatcttatt cgtgaattac gggatgctga atcaccacga cgtcttacct tccgggaaat
    35521 aggcgagaaa tttgaattat ccgagagtac ggtgaatttt atttatcacc atcgccggac
    35581 tgccgaggac gctgtattac gggagttaac gccatgataa agagacttgt atttttcgca
    35641 attttcgttc cggtgttggg ggttggtttt atatttgcga ttacaggaaa cctgtccatg
    35701 atgccagata tttggttctt tatcaggatg tcgctcttcc tgtttattat gaatcttctt
    35761 atcgacatat atatccgtat tactggaaaa cataaatgag aaaaatcacg tccttgtcag
    35821 agttacagga gatgaatatg agtattgaat taagatcgtc ttatgagtat cgcaaatttc
    35881 ttatcgccgg aggcatgaaa ccggaagatg tggaaaaaat cgtttctttt atggataagg
    35941 aatgtgacaa gcgggatgtg tcagaaatta ttatggatga catgattctg gattcagtcg
    36001 tggcgttaag tccgttatgg attgtgcatt ctcttgcgga aattgccaaa ggcaccgata
    36061 aacaggcagc tgttgctgcc cttcagacgc ttaatgaaat gcgtatatct ccacgcccga
    36121 cattaataca tatgattctg tccagcatgg aggataaagc aaatgaatag tctacccgct
    36181 ggatgggcga ggccactaat ggccaggaag catcattttt ttaaaacagg cgaaaatatc
    36241 agtatatgtg gacggtggtt atatctggct cataatcgcg agccggatac atttgaaagc
    36301 cctgatgact gtgccgaatg ccgcagaaga gtgaataagg agaaagataa tggacaatag
    36361 ttctctgttg ttctggtgct tatatatcac atcctttttc ggtgcatttg ttattacccg
    36421 gtggttatgc cggaaaatta tctgcttttt tgataaaaga catccggttg aacgggcggc
    36481 tgatgcgctc attcagcagg ccattgtgtt atatagcggt gaatttttct gccggataac
    36541 aaccagagat ggctggcaca taatgattat tccgccaaca caccatgtcc ggtgggatga
    36601 ggcagaaaag gctttccatg tccgtaaaaa ggtaaatacg gtatgagagg aaaactgata
    36661 tccgccattc atgtggcaaa gcgcgagctt gccctggatg atgaggctta tacattcgcg
    36721 ctgctggccg ccaccggcaa aaccagctgc cgggatatgt caccggatga gttatcccgc
    36781 gtgctggatg ttttcaaaaa acgcggtttt aaagtgcgcc agaagccggt taaccgggcc
    36841 ttaaaaccgg gtacggtgac cgccaaaatt cgtgccatct ggaaggtgat gcaccggcag
    36901 ggctttatct ctgatggtgc ggaaaccgcc cttaaccgct gggtgaaatc gcagacagcc
    36961 gcgcagaacg gcggcgaagg tgtggcaaac tggcagtggc tggagcaaca ccccgccctg
    37021 gcctcagatg tgctggagcg tctcaagcga tggcaccgcc gcaaaatgct ggccgcgatg
    37081 gaaatgcccg aacgcacgct gatggggtat gacgccgttt gcaggcagta tgaaaaatca
    37141 cttccccgtt aaccccaaat cccgccacaa cgcgggattt ttattttaaa cttaccggga
    37201 acgcgagaac cggaggctga tatggcagaa actcagatga gcatgtttgg tggtgacagt
    37261 gagcaactgc acgcccttat cgaccgcctt gatgacatcc ctgatgatat tctcaaaaag
    37321 aactggccgc ggaccctgtc agaactggtt gaagtcaccg gcgcagaact tcagcgtcag
    37381 gggattgagc cggtactggc cggtaaactg gcacgcaagg tggctgcggc tcaggcagcc
    37441 tatatgggcg gacggggtta ttacctgccg gtcggggaat ctctctttgc cgagctgcga
    37501 aacaatgaga tattttcgcg ctgggaccgg ggcgagaaaa ttgaatccct tcgccgccat
    37561 taccggatgt cagaaaccca gatttatacc gtcatacgcg aacagcgccg tctgcatctg
    37621 gcaagaacgc aaccgccact tttctgatat tccgcaaagc ctgccgcctc tccttcaccg
    37681 ttacgctgac tcagagaaca tcatgagaca gcgtaacaat gccaaaactc cccgcaccac
    37741 tgcgtaaaaa gctgattgcc cttgttctgg ccggtgccgg gacgttcacg attgccacgc
    37801 attacaccgg ttactgggaa gggaaagaaa actccacgta tatcgatccc accggtacac
    37861 ccaccatctg ttacggccat accggcccgg atgtgaaacc gggtatgacc ctgacagatg
    37921 aagaatgcct ggaactactg gaaaaggaca tgaaatgggc ctttgcggcc attgatcggc
    37981 gtgttcaggt gccgcttacc cgtggtcaga cggtggcgct ggcttcgtgg atcttctggg
    38041 ccggtgaaac gaactttcgc aactccacgc ttctgcgcct gatcaatgcc gggcagatgc
    38101 ccgcgtcctg taagcagtat atccgctgga tttattcaaa gggggtgaaa ctccccggcc
    38161 ttgaggcccg ccgttcggcg gatgaatggt tatgtcgcta cgacttgccg aaagtctgaa
    38221 ccgcttctgg cgaccgctca tgatagcgct gctgtgtgcg gtactgctgt tacggggtgt
    38281 cctgtggctg cggtgaataa tgccttaccg gccctgattg ccggggcgtg catggcggcg
    38341 ctggggatta tcgctgtact ggttgccgct atatggggaa tgcaccagaa gacgcagcgc
    38401 cttgaagaca ataatcaggt gcttgtgcgt gagcgggatg aggcgcgtca ggtgctggca
    38461 aatcagcagc acaccctgca actcatttca caaatcagtg aggcggccac gaatgaaaaa
    38521 caacagaaca ttcagcacag cgaggggcag cagagcgttg tccgccggtc gctggcggca
    38581 gtgcctgcgg cctctgtcct tgttcctgat gatgtggctg atcgggtgcg ccgggccgtc
    38641 tgtgaaatac gtgcctgtga agccggtgcc gatccccgct gaatggctgg ctgactgcct
    38701 ggtccctcct gcgccggagc cgttcacgtt tggggcatcg gtcacttaca acctgcaact
    38761 gctggcggtg atcaagaact gcaacgtgga caaggccagt attcgtcgtc tggaggcgcg
    38821 gcgacaacat gaatttactg atatggccgg aacgcctgct gttccggcag gaaagacgaa
    38881 gtaaggaaaa aggtatggat gattcagatc gtgctcaggc tgtgatggag cggggatcag
    38941 aacgcgccct ttgtaaccgg ctgacacgta agcgccgggc aatggatgct ccgggcagac
    39001 gtgtctgcgc ggactgtggc ggagagatcc ccgccgcacg tcttgttgct gtgccggatg
    39061 ccatccgttg tgtgaactgc cagaacatca tggaggcccg tcatgtgggt cagcatcgtt
    39121 aaagattatg ttgtgccgat cctttcggcg acggcaaccg ctggcgggat attcatggcg
    39181 ctgatgcgca aaacgtttgt cccccgcgag gcctttgaaa aactctccga ccgcgttgag
    39241 aaggtggaaa cccgcctgtc gtcactgccg acagaagccg aggtcaaccg tctgaatgtg
    39301 gaaatcgtga ccctgcgggg tgaactgaaa accacgaacg ccacgctccg ctctgtctcc
    39361 tatcagaacg aactgctgct ggagcaggct gtaaggaaaa aaacgcaatg agtgatttca
    39421 ttactgaaga tcagcgtctt gtcattctgc ggtcgctggc ggattacaac ggcgaactgg
    39481 gtgaatccgt gctccaggac tgtctggatg attacggtca ccgcgtgtcc cgcgacaccg
    39541 ttcacaccca cattgcctgg cttgccgagc agggactggt gcgtaagcgc acgctcgtta
    39601 acggttattt catcgccgaa ctgaccggcc ggggtcagga tgtggcggaa ggtcgcgcca
    39661 gcgttccggg tgtgaagaaa ccacgcgcaa gggggtaacg atggacaagc cgacgcgtgg
    39721 acgcgtgcgc aaggtggatt tgctccccga cagcatccgt aagccactgc tggaaatgct
    39781 gcgtgaaaaa cgtctgacgc aggtccagat acgcgaggaa atcaaccgtc tgatccgtga
    39841 ggcgggcctg ccggaagagc agcagctttc accggcggcc atcagccggg aagcctcccg
    39901 taatgagctg attgcccgta acctgcgtga tttacgcgag cagacaaaag ccatgatggc
    39961 cgaactggga gacaagccga ccggtgaaac cacagcgctg attctggaga tgtcccgcgc
    40021 cctgatgtac cgccgtctgc gtgccgcgac cgaatccctg aacagtgaca gcgacgtgga
    40081 tatgcgtctt attaaggaca tcctcctgtc agcacagcgt gcggaaagtg ccgccgagcg
    40141 cagcattaag cgtgaaaaag aaatccgggc cgcatttgct gaagagatgg caaacgccgt
    40201 caccgacgag ctgcgcggcg tggacgggat gagtgaacag cttgagtccc gtattaaggg
    40261 gattctgttg ggtaaagcct gatgaataac gccgaagaac tctccccctt actgacgaat
    40321 accgtctcca cccgcaaaat tgatctggcc ggtgaaaaag ccctgctggg cgtggatgtg
    40381 ccggacagcc ttgatttgcc cggtgatatg ccggtatttc tggattatca ggcccgctgg
    40441 tttgaggatg aaagcgaggt ctgcatcgcg gaaaaatccc gccgcaccgg gcttacctgg
    40501 gcggaagccg ggcgtaacgt cattaccgcc gcaaagccta agcggcgtgg tggccgcaat
    40561 gtgttttatg tggggtcaaa gcaggagatg gcgctggaat atatttctgc ctgcgcactg
    40621 ttctcccgtg cctttaacca gctggcagat gctgatgtgt atgagcagac cttctgggat
    40681 cgggataaaa aagaagaaat tctgacctac atgatccgct ttccgaacag tggattcaaa
    40741 attcaggcac tgtcttcccg tccgtcaaac ctgcgcggcc ttcaggggga tgtggtgatt
    40801 gatgaagccg cgttccacga gtcactggat gagcttctca aggcggcaat ggcgctcacc
    40861 atgtggggcg cgcgcgtgcg tattatctcc acgcataacg gcgtcgataa tctgtttaat
    40921 cagtacattc aggaagcccg cgaggggcgc aaggattaca gcgtccaccg cattaccctg
    40981 gatgatgcca ttgcggacgg gctgtaccgt cgtatctgtt atgtcaccgg ccgcgaatgg
    41041 tcaccggaaa gcgagcagaa gtggcgtgat gatctctaca aaaacgcccc gacccgtgag
    41101 gatgccgacg aggaatacgg ctgtatcccg aaaaaatccg gcggtgccta tattcctcac
    41161 gcgcttattg aaatggcgat gctccgcgac atcccgattc tgacgtttga agccccggac
    41221 gacttcatca gccgtgccgc atggctgcgt gaatcggagg ttttaacctg gtgtgaagaa
    41281 catttaaaac cgcttttaga ggcgttaaat ccccgttccc gcttcagctt cggggaagac
    41341 tttgcccgca ccggtgacct ctcctgcttt gtgctgctgg aaatcactga atccctggca
    41401 aaacgcgagg tgtttcgcgt ggagctgcgt aacctgccat atgcccagca ggagcaggtg
    41461 atgatgtaca tcctgacccg cgttccggcg ctggtcggtg cggcgttcga cgccaccggt
    41521 aacggcggct atctggccga agccgcgctg ctggcctttg gcccggacat catcgactgc
    41581 gtgatgttat cgccgaagtg gtacggcgag tggatgccaa aactgaaggc cgagtttgag
    41641 gatcagaata tcctcgtcgc ccgccatcag accacgcttg atgatttgcg ccatgtgaag
    41701 gtggtgaacg gtatcccgca aatcgacaag gggcgtacga aggaccagaa cgcaacggct
    41761 gtgaacgccc gccgccatgg tgattttgcc gtggcgctct gtatggcaaa ccgggcgtca
    41821 tacatggagg gcttcatcct ggatgaatca gcctgtcagg cgctgccgga gcggtcgcgg
    41881 gcaatggagg gcggttatcg tgatgacgat gaggcatatc atgaatttga tcgggggtgc
    41941 tggtagtggg acgcataatt gatcttgacg gaaaaccttt ctcctttgac ccagagatgc
    42001 agagtgccgt gctggatatt ccgcagattg ccagccgtta tattgaacat ccggcctcgg
    42061 gtatcacccc gaaccgggcg gcacagtgcc ttcgcggggc tgaacgtggc gatctgattg
    42121 cccagtccga tctggccgct gacattgaag aaaaggatac ccaccttttt gcagagctgg
    42181 gcaagcgacg tctttccatt cagggcgtgc cctggagtat tgagccgcca ccgaacgcca
    42241 gcgcgaatga gaaaaaggac gcggaaatgc tcgacgaata tctgcattcc gccgactggt
    42301 ttgatgccat gctgtttgat gccaccgatg ccatcctgaa gggctattcc tgcatggaga
    42361 ttgagcacgg gatgctcggt aaaatgcaca tcatccgcgc catccgctgg cgtgacagcg
    42421 ggcatttctg cctaaacccg gatgatttga gcgaactgcg gctgcgtgac ggcagccatg
    42481 ccggggtggc gtttcagccc tttggctgga tagtgcatca gtcacgttca cgcaccggct
    42541 acggtggtgc gacggggctt gtcagaacgc ttatctggcc gttcattttc aaaaactatt
    42601 ccgtgcgcga tctggctgaa tttctggagg tgtacggcct gccgatgaag gtcggtaaat
    42661 acccgtccgg ggcaacgccg gagcagaaaa gcgccctgat gcgggcggtg atggatatcg
    42721 ggcgacgtac aggcgggatc atcccggccg ggatgtctct ggagtttcag gcagccgcga
    42781 acggtcaggc cgatccgttt gaaaccatga tttcgtgggg ggagcgttcc atctccaaag
    42841 ctatcctcgg cggcacgctg accacggaag ccggagacaa gggcgcgcgc tcgctgggtg
    42901 aagtgcataa cgaggtgcgc cgggaaatca gggattctga tttacgtcag ctggccgcca
    42961 cgctgaaccg cgatctggtg tatccgctgt atgccctgaa caccgcccac gccatagata
    43021 tccgtcgcct gccgcgtatc tgtttccaga caaaagagcc gggggatatc accaaaatca
    43081 ccagtgcggt gatgcagctc agtacgggga tggatattcc tgatccctgg gtacgggacc
    43141 agaccggtat tccacagcct gcccccggtg aagccatctt ccgtgtccgg cagagtggca
    43201 atgaacctgc tcagacagac aaagagatgc caccgggaaa acaggagaag acagagcaga
    43261 cagcgctggc tgccagactg ccggaagcaa aaagcacgcc ccgtgatgaa ctggacgaca
    43321 tgggggatgc ggtgcctgcc cgcctgttac aggaggccat agacccggtg cttgagccgg
    43381 tcattgatgc catcagaacg cgggggctgg cggaggcact ggcagacctg cctgcccttt
    43441 accgtgaaat ggatgattcc cgcctgatga cgctgctcag tgatgccatg tttgctgcgg
    43501 aaatgaaggg gatgctggat ggcacagggg attgatttag gttatgccgc cacacttgcc
    43561 tcaaaagagg cggtggcata cttccgcgcc aaaggggcgc atatcagctg gaactggttc
    43621 gaaacagacg cggatgttca tgcccgttca ttcacggcgg caaaagcggc acgcctggac
    43681 gtactgacta cactacaggc ggaagtgcaa cgggctattg atgaggggat ttcacagaaa
    43741 gcatttatcc gcacactgac cccccgcctg caaaagctgg gatggtgggg aaagcagatt
    43801 gtggtggaca gcgctggtaa cgcggaagaa gtgcagctgg gtaatccccg ccgtctggcg
    43861 ctgatttaca acgtgaacac ccgcgtggct tacaatgccg ggcgttacac gcagatgatg
    43921 aacaacacgg acacgcatcc gttctggcag tatgtggcgg tgatggacag ccgcacccgc
    43981 ccggcgcatt ccgcccttaa cgggctggta ttccgctatg acgatccgtt ctggaataca
    44041 cactacccgc ccaatggctg gaactgtcgc tgtcgtgtcc ggccattgtc tcaggcccgt
    44101 ctggatgcga tggggctgtc tgtttcatcc ggtcaggatc atctctccac ccgcaatgtt
    44161 gaggctggcg tggataagca gaccggagaa gtcagagaaa tgccggtgac cacatattca
    44221 gatggcacca gaaccatgac accggatgtg ggctggtcat ataaccccgg ttcggcggcg
    44281 ttcggcacag accaggcgct gatccgtaaa ctgatcgagg tgaaaagccc ggcgctgcgg
    44341 gaaatggtgg ttcaggagat gaacaacagc ccggagcggc aactggcgtt ccgcatctgg
    44401 gcaaaaaaca tcatgaaaac ccggcgaggc ggtaacgata tccgcacgct gggctttatg
    44461 accgaaagta ttgcgcaggc ggtggaaagc cggacgggaa cgccaccggc ccgcctgctg
    44521 gcgatgagcg gtaaaaatgt gctccatgcg gacagcatga aacatcagaa tgacggtatc
    44581 gccctgacgc cggaggattt cgcgcagctt cccgccatgc tggctgcccc cgatgccgtg
    44641 ctgtgggatc atgtccatca gaacttgctt tacatcactg aaacccgtga cggaacggca
    44701 aagattgccg tcaatgcgcc ttatagtgtg aaacgtcagc cggataagct ggatgtggtg
    44761 attaatgcct atagagtgag caggaatact ctgattgatg ctgttcaggg aggtaagatg
    44821 gagatcctcg aagggaaatt gtaagtcggt ggtgggggtc gaacccacat aaacatcctt
    44881 cgggtgaaga tatccctcag gtgccgcttt accagttaag agtacaccga cctgctttta
    44941 ttttaaccag gtagtttcac ggaggcaata tgtcatccat tgatgccgct gtcgttgttg
    45001 acgttacgcg tctccagcgg gtctttgccc ggcttcagtt tgtgggtggt ggaaaagacc
    45061 tggcccgcag tgtggcggcg agtcttctgt catcgtcaga aatggcgttt gaacaggaaa
    45121 aagagccgga cggcgaacgc tggcatgact ggtcagatcc ttaccgcaag tggcgtaccc
    45181 gtaagggata catgcccggc aaaatcctga cgctgaacgg cgatctggcc cgacgcctga
    45241 ccacggatta tggcgacacc tgggcgctga ttggatcaaa tgagccttat gcggccattc
    45301 atcagtgggg tggcctgccc ggtatgccgc cgggaccggc ggccattggt gcacgtccgt
    45361 atatgggctt tgatcaggtg gcagagcagg agatcatgga cgaaatcaga aaacgcttta
    45421 aaaaggccac agaaacgcct taattgttta aggtatgcaa atgcattacc ttaccccctt
    45481 caggcgcgtg tcgtgatttt ctaacctgaa tttaacgggc tttaaaatct gcgtgaagcg
    45541 cctttgtatt tttcccctga aagcacttcc tgaaatcccg caaaacccgc cctgaaaatt
    45601 cccggcctat gctgccggaa tgaagacgaa aaacacgccc aaacttgctt atgccattct
    45661 gaatgccatc agcctgtccg cagacgggga cggtgactgg tgtcagatca tgccagccgg
    45721 tcgggtaaag gcccgtgacg gtcgcccgga aaaaccggcc gagggctggc tgatcaacca
    45781 tgcagccgtt gagcgcatgg tctcccgtgt tgtggcgctc aatcagccgg tgaaaattga
    45841 ctacaaccac cagaccctga ttgagggaca tccggcaccg gctgccggtt ttgtgatggc
    45901 ctcaccggag aattttcgct tcagtgaaga gcgaggtttc gaggtgcgcc cgaagtggaa
    45961 ccctccggcc cttgaacatc tgcgtaataa cgaattcccc tggttttcac cggtgattgg
    46021 ctatgacgag agcaccggcg aacctgtcga acttcggatg ctggctatta ccggtgaccc
    46081 cggtctgacc ggcatgaatc ctgtcgccgc actgtcggcg gatgaccttt ataacgcctt
    46141 aaaccctcct ttaaaggata cctccatgaa tgagcaatta cgccagttgc tgacggcgct
    46201 cggtctgacc gtggctgacg gtgacgaatt tacgccggag ctgggcacgg cagcgctgtc
    46261 tgccctcacc gggatcaaaa cccgtgcgga tgcacacgac aacctgaaaa cacaggtcgc
    46321 cagtctgtcg gcagagctgg aaactgcaaa aggcacaccg gctggcggca gtattgacct
    46381 gacgaaatac gtgcccgttg aaacgtataa cgccctgcgt accgaatacg ttgcgctgtc
    46441 ggcgcagcac ggcagcacca cgctggagca gttgctggac aaggccgagt ccgaaggacg
    46501 catcttcaaa agtgaacgcg gctatctgga acaactgggc gggcagattg gcgtggcggc
    46561 actttctgca cagcttgacg cccgccagcc ggttgcggcc ctgaccggcc ttcagaccga
    46621 caccgtgacc gtgccggata aaaagactgc caccgctgtg ctgtcggctg aagatatcgc
    46681 ggccgccaaa ctgctgggta aaaccgaagc cgagttcctg aaaatgaaag aggaaatgca
    46741 ataatgccta ccccgattac accggcgatg atcaccgccc tgatgacggg ctaccgttct
    46801 gattttcagg ccgggatgtc catggccccg tcgcagtaca aaaaaattgc gatgaccgtg
    46861 ccctccacat cgaaatccaa cacctacggc tggctggggc agttcccgca gttccgtgag
    46921 tggatcggct cccgcgtcat cgagaagatg aaggcctatg gctatgccat cgtgaacaaa
    46981 acctttgaag gcaccgtcgc cattaaccgc gatgacttcg aagacgataa cctcggtatt
    47041 tattccccgt tgttccagga gatggggcgc gctgctgcgg cacaaccgga tgaactggtc
    47101 tttgctgctc ttcgtgacgg gattaatgcg gcctgctatg acggccagaa cttctttgat
    47161 accgaacatc ctgtttaccc caaagtggat ggctccgggg atgcgcagat ggtcagcaat
    47221 atgtttgtgg caaaaaccgg ctctgtcggt gcacaggcgg attacagcgg tcctgcctgg
    47281 tatctgctcg actgttcacg cgcaatcaaa ccgctgattt atcaggatcg ccgtaaggct
    47341 gaactggttg cccagaccaa agtcgatgaa ggtcgcgcat tcaccgataa cgaatttgtg
    47401 ttcggtgctt ccgcccgtcg caatgtgggc tacggcttct ggcagatggc ctacatgatg
    47461 cagtcaccgc tgacgctgga tgcactgtgg cacggctggt cagccatgcg cgaatttacc
    47521 gctgacggtg gccgcaaact cggcattaaa cccacccata ttgttgtccc cacctcgctg
    47581 gaaaaacagg cggtgcagtt gctggagcgt gaactgttcg cagacggaaa cgcaaccgtc
    47641 tctaacgaga tgaagggcaa gctggagctg gttgtcgcgg attatctgta aacggtgagc
    47701 cgggtttacg gcccggcccc tgcggagggc aaatatgaat gaacatcaca ctgtggcagc
    47761 aacagatgat tcaggtcttc aggtcagtgg tgacaattct gtcacggtgc tggcagaagt
    47821 gcgttgcagt cgttcggcgt ttcgtcgtgc ggggttcctg ttcacgcgtg gacgtcagca
    47881 ggttgaggtc accccggagc agcttgcccg actggaggag gagccatgcc tcactgtgcg
    47941 aatactacag acgtctgctg atgatgcggg gagcgtggcg ggtgtggttc atgcagtggc
    48001 cggtacagat t
//
LOCUS       DAZGOB010000021        47719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGOB010000021 DAZGOB010000000
VERSION     DAZGOB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.021"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..676
                     /gene="comR"
                     /locus_tag="ACHLIF_001270"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="ACHLIF_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO9605319.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(738..1277)
                     /gene="ycfJ"
                     /locus_tag="ACHLIF_001271"
     CDS             complement(738..1277)
                     /gene="ycfJ"
                     /locus_tag="ACHLIF_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO9605320.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHLIF_001272"
     CDS             complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHLIF_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO9605321.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3208..3750)
                     /gene="ycfP"
                     /locus_tag="ACHLIF_001273"
     CDS             complement(3208..3750)
                     /gene="ycfP"
                     /locus_tag="ACHLIF_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO9605322.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3773..4798)
                     /gene="nagZ"
                     /locus_tag="ACHLIF_001274"
     CDS             complement(3773..4798)
                     /gene="nagZ"
                     /locus_tag="ACHLIF_001274"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO9605323.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4809..5633)
                     /gene="thiK"
                     /locus_tag="ACHLIF_001275"
     CDS             complement(4809..5633)
                     /gene="thiK"
                     /locus_tag="ACHLIF_001275"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO9605324.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5614..6255)
                     /gene="lpoB"
                     /locus_tag="ACHLIF_001276"
     CDS             complement(5614..6255)
                     /gene="lpoB"
                     /locus_tag="ACHLIF_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO9605325.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6269..6646)
                     /gene="ycfL"
                     /locus_tag="ACHLIF_001277"
     CDS             complement(6269..6646)
                     /gene="ycfL"
                     /locus_tag="ACHLIF_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO9605326.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6649..7008)
                     /gene="hinT"
                     /locus_tag="ACHLIF_001278"
     CDS             complement(6649..7008)
                     /gene="hinT"
                     /locus_tag="ACHLIF_001278"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO9605327.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7342..9531
                     /gene="fhuE"
                     /locus_tag="ACHLIF_001279"
     CDS             7342..9531
                     /gene="fhuE"
                     /locus_tag="ACHLIF_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO9605328.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLIGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HIKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9591..11024)
                     /gene="ptsG"
                     /locus_tag="ACHLIF_001280"
     CDS             complement(9591..11024)
                     /gene="ptsG"
                     /locus_tag="ACHLIF_001280"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO9605329.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11319..12116)
                     /gene="ycfH"
                     /locus_tag="ACHLIF_001281"
     CDS             complement(11319..12116)
                     /gene="ycfH"
                     /locus_tag="ACHLIF_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9605330.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12127..13131)
                     /gene="holB"
                     /locus_tag="ACHLIF_001282"
     CDS             complement(12127..13131)
                     /gene="holB"
                     /locus_tag="ACHLIF_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO9605331.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13128..13769)
                     /gene="tmk"
                     /locus_tag="ACHLIF_001283"
     CDS             complement(13128..13769)
                     /gene="tmk"
                     /locus_tag="ACHLIF_001283"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9605332.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13759..14781)
                     /gene="yceG"
                     /locus_tag="ACHLIF_001284"
     CDS             complement(13759..14781)
                     /gene="yceG"
                     /locus_tag="ACHLIF_001284"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO9605333.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14784..15593)
                     /gene="pabC"
                     /locus_tag="ACHLIF_001285"
     CDS             complement(14784..15593)
                     /gene="pabC"
                     /locus_tag="ACHLIF_001285"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO9605334.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15713..16954)
                     /gene="fabF"
                     /locus_tag="ACHLIF_001286"
     CDS             complement(15713..16954)
                     /gene="fabF"
                     /locus_tag="ACHLIF_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO9605335.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17042..17278)
                     /gene="acpP"
                     /locus_tag="ACHLIF_001287"
     CDS             complement(17042..17278)
                     /gene="acpP"
                     /locus_tag="ACHLIF_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO9605336.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17489..18223)
                     /gene="fabG"
                     /locus_tag="ACHLIF_001288"
     CDS             complement(17489..18223)
                     /gene="fabG"
                     /locus_tag="ACHLIF_001288"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO9605337.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18236..19165)
                     /gene="fabD"
                     /locus_tag="ACHLIF_001289"
     CDS             complement(18236..19165)
                     /gene="fabD"
                     /locus_tag="ACHLIF_001289"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO9605338.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLIASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVINFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19181..20134)
                     /gene="fabH"
                     /locus_tag="ACHLIF_001290"
     CDS             complement(19181..20134)
                     /gene="fabH"
                     /locus_tag="ACHLIF_001290"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO9605339.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20202..21272)
                     /gene="plsX"
                     /locus_tag="ACHLIF_001291"
     CDS             complement(20202..21272)
                     /gene="plsX"
                     /locus_tag="ACHLIF_001291"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO9605340.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21353..21526)
                     /gene="rpmF"
                     /locus_tag="ACHLIF_001292"
     CDS             complement(21353..21526)
                     /gene="rpmF"
                     /locus_tag="ACHLIF_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO9605341.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21578..22099)
                     /gene="yceD"
                     /locus_tag="ACHLIF_001293"
     CDS             complement(21578..22099)
                     /gene="yceD"
                     /locus_tag="ACHLIF_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO9605342.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22298..22882
                     /gene="yceF"
                     /locus_tag="ACHLIF_001294"
     CDS             22298..22882
                     /gene="yceF"
                     /locus_tag="ACHLIF_001294"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO9605343.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22994..23953)
                     /gene="rluC"
                     /locus_tag="ACHLIF_001295"
     CDS             complement(22994..23953)
                     /gene="rluC"
                     /locus_tag="ACHLIF_001295"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO9605344.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24526..27711
                     /gene="rne"
                     /locus_tag="ACHLIF_001296"
     CDS             24526..27711
                     /gene="rne"
                     /locus_tag="ACHLIF_001296"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO9605345.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27907..28860)
                     /gene="flgL"
                     /locus_tag="ACHLIF_001297"
     CDS             complement(27907..28860)
                     /gene="flgL"
                     /locus_tag="ACHLIF_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO9605346.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28872..30515)
                     /gene="flgK"
                     /locus_tag="ACHLIF_001298"
     CDS             complement(28872..30515)
                     /gene="flgK"
                     /locus_tag="ACHLIF_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO9605347.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30581..31522)
                     /gene="flgJ"
                     /locus_tag="ACHLIF_001299"
     CDS             complement(30581..31522)
                     /gene="flgJ"
                     /locus_tag="ACHLIF_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO9605348.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31522..32619)
                     /gene="flgI"
                     /locus_tag="ACHLIF_001300"
     CDS             complement(31522..32619)
                     /gene="flgI"
                     /locus_tag="ACHLIF_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO9605349.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32631..33329)
                     /gene="flgH"
                     /locus_tag="ACHLIF_001301"
     CDS             complement(32631..33329)
                     /gene="flgH"
                     /locus_tag="ACHLIF_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO9605350.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33382..34164)
                     /gene="flgG"
                     /locus_tag="ACHLIF_001302"
     CDS             complement(33382..34164)
                     /gene="flgG"
                     /locus_tag="ACHLIF_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO9605351.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34302..35057)
                     /gene="flgF"
                     /locus_tag="ACHLIF_001303"
     CDS             complement(34302..35057)
                     /gene="flgF"
                     /locus_tag="ACHLIF_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO9605352.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35077..36282)
                     /gene="flgE"
                     /locus_tag="ACHLIF_001304"
     CDS             complement(35077..36282)
                     /gene="flgE"
                     /locus_tag="ACHLIF_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO9605353.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36307..37002)
                     /gene="flgD"
                     /locus_tag="ACHLIF_001305"
     CDS             complement(36307..37002)
                     /gene="flgD"
                     /locus_tag="ACHLIF_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO9605354.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37014..37418)
                     /gene="flgC"
                     /locus_tag="ACHLIF_001306"
     CDS             complement(37014..37418)
                     /gene="flgC"
                     /locus_tag="ACHLIF_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO9605355.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37422..37838)
                     /gene="flgB"
                     /locus_tag="ACHLIF_001307"
     CDS             complement(37422..37838)
                     /gene="flgB"
                     /locus_tag="ACHLIF_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO9605356.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37993..38652
                     /gene="flgA"
                     /locus_tag="ACHLIF_001308"
     CDS             37993..38652
                     /gene="flgA"
                     /locus_tag="ACHLIF_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO9605357.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38728..39021
                     /gene="flgM"
                     /locus_tag="ACHLIF_001309"
     CDS             38728..39021
                     /gene="flgM"
                     /locus_tag="ACHLIF_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO9605358.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39026..39442
                     /gene="flgN"
                     /locus_tag="ACHLIF_001310"
     CDS             39026..39442
                     /gene="flgN"
                     /locus_tag="ACHLIF_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO9605359.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39482..41017)
                     /gene="murJ"
                     /locus_tag="ACHLIF_001311"
     CDS             complement(39482..41017)
                     /gene="murJ"
                     /locus_tag="ACHLIF_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO9605360.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGTMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVM
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41127..42050)
                     /gene="yceM"
                     /locus_tag="ACHLIF_001312"
     CDS             complement(41127..42050)
                     /gene="yceM"
                     /locus_tag="ACHLIF_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9605361.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTVLWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42052..42699)
                     /gene="yceH"
                     /locus_tag="ACHLIF_001313"
     CDS             complement(42052..42699)
                     /gene="yceH"
                     /locus_tag="ACHLIF_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO9605362.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42710..43294)
                     /gene="rimJ"
                     /locus_tag="ACHLIF_001314"
     CDS             complement(42710..43294)
                     /gene="rimJ"
                     /locus_tag="ACHLIF_001314"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO9605363.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43530..44738
                     /gene="mdtH"
                     /locus_tag="ACHLIF_001315"
     CDS             43530..44738
                     /gene="mdtH"
                     /locus_tag="ACHLIF_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO9605364.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44802..45449
                     /gene="grxB"
                     /locus_tag="ACHLIF_001316"
     CDS             44802..45449
                     /gene="grxB"
                     /locus_tag="ACHLIF_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO9605365.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45583..46143
                     /gene="yceB"
                     /locus_tag="ACHLIF_001317"
     CDS             45583..46143
                     /gene="yceB"
                     /locus_tag="ACHLIF_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9605366.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46249..47295
                     /gene="pyrC"
                     /locus_tag="ACHLIF_001318"
     CDS             46249..47295
                     /gene="pyrC"
                     /locus_tag="ACHLIF_001318"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO9605367.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47369..47614
                     /gene="dinI"
                     /locus_tag="ACHLIF_001319"
     CDS             47369..47614
                     /gene="dinI"
                     /locus_tag="ACHLIF_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO9605368.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11542 a        12918 c        11857 g        11402 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgctggaaa aactaatgca gattgccgga acgactttgc gtttatggcc
      661 cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat
      721 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccatt actatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cgcgaccacc gccaaactga
     1021 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa
     1381 tgattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1441 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
     1621 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
     1741 atttttcagc ggcttaccat tcatctgcgc cagaatgttg ttcattgcgc aagtcgccat
     1801 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1861 gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac
     1921 cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccacacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
     2041 gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
     2161 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag
     2341 attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
     2461 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc
     2521 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgccact tcgtgcagca gcggtttcca cagatggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg tgaccccctt
     2821 aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt aacaacaggt
     2881 tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc
     2941 aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc gggtgagatc
     3001 aaaaaacaga gtttattgtt tattatttga gcaagtccgg aagggcgata tagggtgaag
     3061 cgtatatcga gggcgtttgg tcgtcctgat aagaggcgca tcaggcattg atgctcgaat
     3121 gtcggatgcg gcgtgaacgc cttatccgac ccacagaatc cggagcgcat caggtaccgg
     3181 caaggttacc tgatgcgacg agggcattta cccgagggtt ttgaacgctt taatgcgctg
     3241 taaatgcggg gagatattct tgaatttgtg cgtctgctct tcgtcccaga caatctcgta
     3301 ataatgatgc agctcttcag atgtccgctg gctgttaagc gcttcatcat tacgcgacaa
     3361 aatcaccagg caacgatcgc gattcttctc acggaagttg gtcacacact tagtggcaat
     3421 atcggcatac tcttccgggc gatcaatctt gccttccatg ttctcataag ggaacaaatt
     3481 agggttgaag atcacctggc ggatatcgca aagaaaacca atccgttccg cccagtatcc
     3541 gcccagacca acgccgcaaa tcagcggacg ctcgtcgacg ttcagttgca acattttgtc
     3601 cacttctttg agcagatgct gcatatcatg tttcggatgc cgcgtactgt agcttatcaa
     3661 gcgtacatcc gggtcaataa actgcaattg taagactttc tcgtggttac ccggactgtt
     3721 agagtcaaaa ccgtgtaaat agataatcat cgcttcctca cataagccag ggttagtgac
     3781 ctgctttctc ttcctgccag cgttcatgta actgattcag acgggtgctg atcgctttcc
     3841 agcgagccga gtccatcagt tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa
     3901 cacgttctgc cttgatcggt gacagattat ctaacacgct gacggcccct ttacgattat
     3961 tgcagaccag gatcatatcg caacccgcat ccagtgatgc ctgcccgcgt tcggcataac
     4021 tgcccataat cgcggcacct tccatcgata aatcgtcaga gaaaatcacg ccgtcaaaac
     4081 ccagttcctg acgcaaaacg gttttcagcc agtagggaga accgctcgcc ggacgcggat
     4141 caacatcact gtagatcaca tgcgcaggca taatggcgtc gagtttattt tcgcggatta
     4201 aggaactgaa gaccgacata tctttagcgc gaatctccgc ttgtggacgt ggatcgcacg
     4261 gtgtttcttt gtgtgagtct gccgttactg caccgtgtcc tgggaagtgt ttcccggtcg
     4321 ttttcattcc ggcttcatgc ataccatcaa taaaccggct ggcaattgcc agggcttttt
     4381 gtggatcggc atgataagaa cgctcgccaa tcgccgcgct gatatgcccg acatccagca
     4441 ctggcgcaaa gctgatatca atatccatag cgatcatttc gctggccatc agccaacccg
     4501 cctcttgcgc cagtttgccg ccctcttcca ttcctgacag cgcagcgaat gattgcgccg
     4561 ctggcaagcg ggtaaaacct tcacgaaaac gctgcacgcg tccaccttcc tgatctaccg
     4621 ccaccaccag atgattgcgc gaagctgcgc ggatctggcg caccagttca cgcaactggg
     4681 caggatcatg atagttacgc gtaaagagaa tcagccctcc caccagcgga tgcgcaagta
     4741 tttcacgctc ttccgcgtcc agttcgtaac cttcgacatc caacattact ggacccacac
     4801 tgctctcctt attgttttat taatagctgc cgccaggtgt catcggccag cctgataaat
     4861 tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc ctgctttgag catcagcagc
     4921 cagggaaacc atcgcctgac ctgacgccat aattgcgccg gataaatctt cgcgcgagtg
     4981 gcatagtcat tgaccaattg ccggtgctgt tcagtatttt ccacccacac tgccgccagt
     5041 tccagcgcga tatcaccatc tccggcatac tcccagtcga tgagttttaa ccctgacgcg
     5101 ctatgcacta aatttccggc gtggacatcc atatgcaatg gacttaagcg taaaggccgt
     5161 ggttcccgcg ctttgcgcag acgttttaac attcgcagcc aacccactgt ccgccgcgcc
     5221 ggatcgcttt gctgccagta cagttccagt aacggcaaca gcgttattcg ccagccaaaa
     5281 cgtggttgtt gatgtagata atacagcaag cctgccagtt cgttggtatc cggcaaatac
     5341 gtttttacct cgccgggcag atagtcgact accatccagt cacggagata taaatgcggc
     5401 ttcggtgcaa tgcttgcggg tagttgtgat aaagcccgat actggcgcaa gaacgcggac
     5461 tgcggcgcat caggatcgtg cggctgacgc acaacaaaac gctggccctg atgttcaatg
     5521 agaaaactcc cgccactaag cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa
     5581 aagcgcgaca gcaattcgtc gcgcatgatg ggattattgc tgcgaaacgg cacctttacc
     5641 tgaccagata atttcgcccg tctgcaccag catcagctgc atttgtaggg tcggagcgtt
     5701 aacgttgcca gaggcgctgg agtacagcac gtaatgagcg ccgacattgc gggcaatgcc
     5761 tatggcttta ctacgggtac ctaaactgtc ctgcggcgac aaacctaact gttgcttagc
     5821 catcgacagc tgctgggcgg aaaccagggt aaatttcccg ttattagcca gcgcatttcg
     5881 cagcgtttcg gtcgcttctg cggcattcag cgaaccgtta gtacggttgt taacgctatc
     5941 aaccagcagg acgctacccg cagtcacccc gtcagcccca agcatcttac tgaccatcgg
     6001 ctgcattgcg ccattccagt cataatggcg aatatgcggc gcaggcggtg ccgtttgatc
     6061 ttcgtgctca attgggcctg gctgctgcgg gatcgtcggc accgagggca ctgtggggac
     6121 aggctgttgt ggctcggctg gttgttccgg cgctggtttc acttcttcta ccggtgcagg
     6181 ttcacgttgc cccacacacc cggcgagaaa catcgccagc gcggtaatca aggcgtagcg
     6241 actcattttt gtcatcaaga ttcacccctt acaaatatag ataaagtctg actttgtgcg
     6301 cccccagaaa attggcgctg ccgtacagcg ttaccgccga atgtgcggga atggtaacgc
     6361 tgcgtggcct ttccagagga tgcatctcca gccctctggc gtcataccag taaaaacgat
     6421 aatgaacggt aacgggttct tgcctttcgt tataaagcgt tgaggatgct gaaggttgaa
     6481 tatcagacgt cgaaaggacg ggcttttctg cactgatgcc cgcagccaat aaagatgact
     6541 ccatcaccag cgattgctca tcattcaccg gaatttccgg atgtgaacga cagcccacca
     6601 gcagtaacaa cgccagagac accagcccaa agcatccttt tctcatcgtt acagaccttt
     6661 atgtgccagc attggtccca gcggacggcc acccaacaag tgcatatgga tgtggtaaac
     6721 ctcttgtccg ccatggcggt tggtattcat gatcagacga tagccatctt cggcaatacc
     6781 ttcttgctca gcaatttttg ccgctacggt gatcatgcgt cccagcgcct gctcatgctc
     6841 agctgacacg tcgttcacag tcggaatgag gatattcgga atgatcagaa tatgcgttgg
     6901 cgcttgcggc gaaatatcgc gaaacgccgt taccagatca tcctggtaga cgatatcgga
     6961 ggggatctca cgacgaataa ttttgctgaa tatagtttct tctgccacga cattttcctt
     7021 tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca cactgcgagt atagagtatg
     7081 gtcgagttaa acctgctctt tcaaccttaa ctcacgattt cttaagcaaa aaatcgtttt
     7141 gctgccgtat atatcgccat tattcccatt tctgcctgcg aatgcgctga aaagacattc
     7201 tctgtttggt ttacacgtca aatatggatg cgtatatttc tcatttgcat ttacatacaa
     7261 aattattcgc acactaaata taaccgctca tcaccctcct ccggcatgag cctgacgaca
     7321 acataaacca agagatttca aatgctttca acacaattta acagggataa tcaatatcaa
     7381 gccagcacca aaccatcact acttgccggt tgcatagcat tggcactatt accttctgcc
     7441 gcttttgccg ctccagccac tgaagaaacg gtgattgttg agggttcagc cacagctcca
     7501 gatgatggcg aaaatgatta cagcgtaacg tctacctctg cgggtaccaa aatgcagatg
     7561 actcaacgtg atattcctca gtcggtcact attgttagcc agcagcggat ggaagatcag
     7621 cagttacaaa cgctgggcga agtgatggaa aacacgctgg ggatcagcaa aagtcaggcg
     7681 gattccgatc gtgctcttta ttattcccgc ggattccaga tcgataacta tatggttgat
     7741 ggtatcccca cctattttga atcgcgctgg aatctgggcg acgcactttc tgatatggca
     7801 ctctttgaac gcgtagaagt agtgcgtggc gcgacaggac tcatgaccgg gacgggtaat
     7861 ccatctgcgg caattaatat ggttcgaaaa cacgcgacca gtcgtgaatt taaaggcgat
     7921 gtctcggcgg aatacggtag ctggaacaaa gaacggtatg tggcggattt acaaagccca
     7981 ctcaccgaag acggtaaaat ccgcgcgcga attgtcggcg gctaccagaa taacgactca
     8041 tggctggacc gctacaacag tgaaaagacc ttcttctccg gcattgtcga tgctgattta
     8101 ggcgatctta cgacgctttc agccggttac gaatatcagc acattgatgt taatagccca
     8161 acctggggcg gtttaccgcg ctggaatact gatggcagca gcaacagtta cgatcgcgca
     8221 cgcagtaccg cacctgactg ggcgtacaac gataaagaga tcaacaaggt ctttatgacc
     8281 ctgaagcagc ggtttgctga tacctggcaa gcgacgctga atgccaccca ctctgaagtc
     8341 gaatttgaca gcaaaatgat gtatgtcgat gcctatgtaa acaaagcgga tggtatgctg
     8401 attgggccat acagtaatta tggacctggc tttgattatg tcggcggcac cggttggaac
     8461 agtggcaaac gtaaagttga tgcgctggat ttgttcgctg acggtagtta tgaattgttt
     8521 ggtcgtcagc acaatctaat gtttggtggc agttacagca aacaaaacaa tcgttacttc
     8581 agttcatggg ccaacatctt cccggatgaa attggcagtt tctacaactt taatggcaat
     8641 ttcccacaaa ccgactggtc accacagagc ctggcgcagg acgataccac acatattaaa
     8701 tcgttatatg ctgccactcg tgtcaccctt gccgatccgc tgcatctgat cctcggcgca
     8761 cgttatacca actggcgggt tgatacgctg acttacagca tggagaaaaa ccacaccacg
     8821 ccttacgctg gtctggtgtt tgacatcaat gacaactggt cgacctacgc cagctatacc
     8881 tctattttcc agccgcaaaa tgatcgtgac agttcaggca aatatctggc tccaatcacc
     8941 ggtaacaact acgagctggg tctgaaatcg gactggatga atagccgtct gaccaccacg
     9001 ttagccatct tccgtattga gcaggataat gtcgctcagt ccaccggtac acctatcccc
     9061 ggcagcaacg gcgaaaccgc ctataaagca gtggatggga cagtcagtaa aggggtggaa
     9121 tttgaactca acggcgcaat taccgacaac tggcagctga catttggcgc aacgcgctat
     9181 attgcagagg ataacgaagg aaacgccgtt aatcctaatc tgccacgcac cacggttaaa
     9241 atgttcacca gctatcggtt gcctgtcatg ccagagttga cagtcggcgg tggtgttaac
     9301 tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg gcaccttccg cgccgagcaa
     9361 ggtagctacg cgctggtgga tctcttcacc cgctaccagg tgacgaaaaa cttctcgtta
     9421 caggggaacg tcaataacct gttcgacaaa acctacgata ccaacgtgga aggttctatc
     9481 gtctacggcg caccgcgtaa tttcagcatt accggcacgt atcaattctg aatagcacct
     9541 gtaaaaaagg cagccatctg gctgccttag tctccccaac gtcttacaga ttagtggtta
     9601 cggatgtact catccatctc ggttttcagg ttgtcggatt tagtaccgaa aatcgcctga
     9661 acaccagaac cagcaaccac tacgcccgct gcgcccagtt tcttcaggcc agcctgatcc
     9721 actttagaca catcagcaac gctgacgcgc agacgggtaa tacatgcgtc gaggttagta
     9781 atgttttctt taccaccaaa tgcagcaacc agagccggtg ccatttcgct ggtacctgtc
     9841 gcttttgcat cttcagtcgc gtcttcacga cccggcgttt tcagatccag tgctttaatc
     9901 agcacgcgga agatggtgta gtaaacaatc gcataaccga taccgacgat cgggaacagc
     9961 cacagtttgc tgctgttacc agacagaacg atgaagtcga tcagaccgtg cgagaacgac
    10021 gtaccgtcac gcatccccag aagaatacag attgggaatg ccaggcctgc cagaatcgcg
    10081 tggatgatgt acaggatcgg cgcaacgaac atgaaggaga actcgatcgg ctcggtgata
    10141 ccggtcagga acgaggtcag cgccgcggag atcataatac cgcccacttt cgcgcggttt
    10201 tctggtttag cagagtgcca gatagcaatt gcggcagctg gcagaccgta cattttgaac
    10261 aggaagccac cagacagttt acccgcagtc gggtcacccg ccatataacg cggaatgtcg
    10321 ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa tctgcatctg gaaaggtacg
    10381 ttccagatgt ggtgcagacc aaacggtacc aggcaacgtt cgatgaaacc gtaaatgcca
    10441 aacgcaacta ccgggttctg gtaagcagcc cactgagaga aggtctggat tgcagaacca
    10501 atcggcggcc aaatgaagga cagcacaacg ccagtaaaga tggcagccag gccagaaatg
    10561 atcggcacaa agcgtttacc ggcaaagaag ccaagatact caggcagctt aatacggtag
    10621 aaacggttaa acatgtacgc tgcgatcgca ccggagataa tccctccgag tacgccagta
    10681 tccgccaggt gtttagaggc gatttcttca gcaggtaaat gcagtaccag tggcgcaacc
    10741 acggccatgg ttttaaccat gatgccatag gcaacaactg cggccagcgc ggatacgcca
    10801 tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa tcagtggcat gtttgcaaag
    10861 acggaaccgc ctgcttctgc cataacatgc gatacaacgg cgggcagcca gctgaaattc
    10921 gcggaaccga cgcccagcag aatacctgcg ataggcagta cggataccgg cagcatcagc
    10981 gatttaccga ccttttgcag gttagcaaat gcattcttaa acataattga gagtgctcct
    11041 gagtatgggt gcttttttta cgttctcacg cgtggcaagg ggggagagcc tcgccgtgta
    11101 cagggcatct aagcgccctt tatttattac acagagtaaa ataattcagt gccaatttgt
    11161 ttgacggcta tcacgtttca acttcagatc attggagaag ttgcacattt tgacaaaaca
    11221 tatgtcacaa tatttcaaac gtggttattc accgcccaat tttcggcggt gaactttact
    11281 cgttttagcc attaattacg agctttaaaa aaactcattc aacggatgga ttgaaggcgg
    11341 gaagcgtcga tgtgaaacag acgggcgaag ttatcggtgg ttacctgcgc cagttcttca
    11401 acggcaacac ctttcaacac agccatgtat tctgcaacgt cacgaaccat cgcaggttga
    11461 ttctcttttc ctcgatgcgg taccggcgca aggtaaggtg agtcagtttc caccagtaat
    11521 cgatccaggg ggacataacg cgcagcatcg cgcagttgct ccgcattacg gaaggtcaca
    11581 atgccggaaa aggagatgta aaatccgaga tccagtaatt tacccgccgt ttctctgtcc
    11641 tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc
    11701 agcgtatcgg cacgggcgtc acgggtatgg acgataaccg gcttgttcag ttcacgacca
    11761 atctggatat gatggatgaa ggactcttgc tgacgtactt tagtttccgg tgtgtaataa
    11821 taatccagcc cggtttcacc cagcgctaca acaccctctt ctgccgccag acggcgtaaa
    11881 tcttctacat cgtagggatc attctggttg agtggatgca cgccacaaga aaatacgacg
    11941 ttgtcacgtt cgcctaccag atcccgcata tgtaaataac ccggtaatgt tgtggcgact
    12001 gccagacaaa atttcacatc gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta
    12061 tgcaaagatt cataatccag accatcgaga tggcagtgtg agtcgactaa aaacatgatg
    12121 tctctcttaa agatgaggaa ccggtagcac aacgcccggt tgcaggtaat gctcaatacg
    12181 cagtaaaaga tcggtgatga gaagctcgcg gttgatgcct gtaacagaca ttaactgttc
    12241 acgaatgtgg caaacatccc ccagtatagc ctgcaggcgc gagggagaaa gatggtttgc
    12301 cagttcggcg accaggcccg gcacatcaac attggtcacc tgcgcagcac catgatggcg
    12361 ttttagcgca tccatcagca acgttgccag ccagtgtaaa cgcgccggag cttgttcatg
    12421 attaagggcc gctagcagcg aataccagtc gcccgatggc acgctatatg ccaacgcctg
    12481 acacaatgtt tcacgagcct gccagttatc tccctgaaac aacgccagtg ccgcgccagg
    12541 cgaaccggcg cttaagcgca atgcggcaag taatgcatcc tgtgacattg tcacttcgcg
    12601 tgaaagccag gtcacggcgt actgttccgg cggcggcgca aggtaatgta accgacaacg
    12661 actacgtaat gttgccagta aacgttcagg ctcgcgggta gccaggaaaa accaggtttc
    12721 tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc gcggcgtcgg tcagtaaggc
    12781 agcatcggtt acccagacaa ctttcgcacc acctaggcgt gcgtgctcat tcagcttttc
    12841 ggtgacctca cgtaccgcat caatgcccag catatttttc cctttttcgg gagccagggt
    12901 gtagtaatcg ggatgcgtgc cagcctgcat caactgacat ccacgacagt gaccgcaact
    12961 tttgtggccc tgcggttgtt ggcagagtaa ataacggctc agggcgtaga ttaaagcatc
    13021 atcgcccatg cccggtaacg cctgaatgag tagcgcatgg tgacctcttc cggcctgata
    13081 gctggctacc agtttttcga aatcaggtcg taaccatgga taccatctca tgcgtccaac
    13141 tccttaaccc agtgggtcac ggtagtgcgg attgcatcca tcacggcctc cagcggctgg
    13201 gtggcatcaa tggtatgaat acttttatct tgtgcagcca gttccagata gcgggcgcgg
    13261 gtgcgattaa agaaatcgaa agactcttgc tcaatacgat ccagctcgcc gcgcgcacgc
    13321 gcgcgtttta agccaacttc cggggtaaca tcgagataga gcgttaagtc ggggcgaaaa
    13381 tccccgagaa cagcatcacg cagtgttgcc agcatatgtt ggtcaatacc acgtcctccg
    13441 ccctgatacg cctgagtgga gagatcgtgg cgatcgccaa tcacccaggt gccgttagcc
    13501 agagctggtt tgatgaccgt ttctaccagt tgaacgcgcg cggcataaaa catcagaact
    13561 tcggctttat cggtaatgac ttcatcgcct accgatttga tatccagcac caggcttctt
    13621 aacttttcgg caagttgcgt accgccaggt tcccgagtga aaaccatgtc gcggataccc
    13681 agttgctcaa gcgtctcaac caccacatta cgcgcggtag ttttgcctgc gccttccagc
    13741 ccctcaatga cgatatactt actgcgcatt tttttcctta agcactttca gataatcctg
    13801 cacagacttg ttatggctgg caagattggt attaaacgtg tgaccacctt taccatcggc
    13861 cacaaaatag agatacggcg tttttgccgg atgcgcagca gccttcagcg aatccgcccc
    13921 cggcgtagct atcgcacccg gcggcaaacc ggtaatggta taggtgttat acgctgtcgg
    13981 cgtttccagg tctgcacgag aaagtttgcc attataacgc tctcccatcc cgtaaatcac
    14041 ggttgggtcg gtctgcaggc gcataccaat gcgtaaacgg ttgataaata ccgaggcaac
    14101 ctgatcgcgt tcactggcaa cggcggtttc tttttcgata attgatgcca tcgtcaccag
    14161 ctgattttta tctttataag gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc
    14221 tttcaccatt ttcttgtgcg ctcgcttgag taacgcgaca tcggtggtat tggcggtata
    14281 catccaggtg tctggccaga accaaccttc aatccactcc gggttttcca gttcaagtgc
    14341 ctgcgctacg gtggcgtact tatcgtcact cagcgtgtgc ttgatatacg gagcctcacg
    14401 caattgcttg aggtagtcgc tcagacgcat cccttctacc agtcgcagcg ggaactgtgc
    14461 ttctttaccg ctttccaaca atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg
    14521 gtaagtcccg gctttaaagt gagaaagatc cggttcgata cgcagcagcc attgaaaaac
    14581 ccgtgggcga ttgatgatct tatcagcata aagctgttca ccgagcgcca gacgtccggt
    14641 ccctggcttc agggtaaata tcgtctcttc tttgataagc aatttgctgt cggcaagatg
    14701 gcgaaccttc cagacgccca caccagcggc gatacccagt accaccagca ataacaagat
    14761 aatcaataac acttttttca tgactaattc gggcgctcac aaagtggggc taaatattca
    14821 tataacgttg ctgacgaaaa ggagacatcg ccacaggcac gtacgggcat cactggcatt
    14881 aacgcattac aaataaccat ctcatctgcc tgcaacgcct cttccagaga ggcttgcact
    14941 tcgacaagct gataagagga ttgtgccagc aaacggatac agaattgtcg cataatgccg
    15001 ttaacacctg cctgatccag tcgcggcgta taaactacat tgcccttccg ccagaacaaa
    15061 ttagccgcac agcattccgt aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg
    15121 tttgtctgct caagatgaga gcggatcaat acttgctcaa gccgattaag atgtttaata
    15181 cctgcaagat gaggattgcg ccccagccgc accgggctta gcgccaacgt catcccttcg
    15241 ttacgcaaac ggtcgtaatg tgcaggataa accgtaacgg agagaatccg cgttgccggt
    15301 ccactgttca atgtgctgta ccctcgcccg ccactaccgc gactgatcac gactttcagc
    15361 acaccatttt gctgttctgc tgccagcgtt ttcatctctt gttcaagctg aggccagaag
    15421 tcacagaaaa tcatcaaccg ctgacaagca tcctgtagtc gctggatatg cgccgataac
    15481 aaactgactt taccgtcgat aactctggcg gtggtaaaac aaccatcacc aaactgcgtt
    15541 gcccgatcgc ttactgccag cgattcctgc ttataaccgt taattaagaa cataccggct
    15601 ccttatcaga gaattgttag tgtggcagca tgttcactac ggaacaagtc ggaataaaag
    15661 ctaagaaaaa aggcccgcaa gcggaccttt tatatgggtg gaaaatgaca acttagatct
    15721 ttttaaagat caaagaacca ttagtgccac cgaagccgaa ggagttacac agcgtgtatt
    15781 ccattccgct gacctgacgt gcttcatgcg gtacgaaatc cagatcgcaa ccttcatccg
    15841 ggttatccag gttgatggtt ggcggaacag cctgatcgcg cagcgccagg atggagtaga
    15901 tagattctac tgcacccgcc gcacctaaca ggtgaccggt catagatttc gtggagctta
    15961 ccaacacacg gcttgcagct tcaccgaaga tggttttcac cgcctgcgct tcagctttat
    16021 cgccagccgg cgtagaagta ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc
    16081 ctgcatcacg cagagcattt gccattgcca gagctgcgcc tgcgccattt tctggcggtg
    16141 acgtcatatg ataagcatcg ctgctcatac caaagccgac gagttcagcg taaattttcg
    16201 caccgcgttt tttcgcgtgt tcgtactctt caagtaccag cataccggca ccatcgccca
    16261 gtacgaagcc atcacgctct ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc
    16321 gggtagataa tgcacgtgcc gcgccaaaac caccaacgcc cagcggcgta ctggctttct
    16381 ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc gataatacgc gcagcatgac
    16441 caatgttgtg cacgccggaa gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc
    16501 catacatgat agtcagatga cctgccacca tgttcacaat cgttgacgga acgaagaatg
    16561 ggctgatctt acgtggacca ccgttcatca gagatgtgtg gttttcttcg atcagtccga
    16621 ggccgccaat cccggagcca attgcggcac caatgcgggt tgcgttctct tccgttattt
    16681 caaggccaga atcctgcatg gcctgaacgc cagcgacaat tccatattga atgaaggcat
    16741 ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca gttaaaatcc tttactaagc
    16801 cagcaaattt cgttgcatag gcgctagtat cgaaatggtc gattaggctg atgccactct
    16861 gaccggcaag cagagctttc caggtagact ctacggtatt gccgacagga gacaacatgc
    16921 ccagtccggt cacaactaca cgacgcttag acacgtttgt cctccaggga gggaaaaaat
    16981 gattctagtg ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca
    17041 cttacgcctg gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag
    17101 cttcttcgtc cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg
    17161 tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct
    17221 gcttaacgcc cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac
    17281 tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga
    17341 aaaatttgca actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta
    17401 ccccccaata acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg
    17461 caacatgagc attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca
    17521 aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga
    17581 tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt
    17641 catcgctcag cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag
    17701 taataccgcg tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt
    17761 tcgccgcagc gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag
    17821 tgataatacg accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga
    17881 aaacagatga aaggttggtt tcgataattt cgttccactc ttcatctttc attcgcatta
    17941 acaggttatc acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg
    18001 cgcgaatttt ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac
    18061 ctttgccgtt ggcacctaaa taatcgctga tcgcctgagc gccattttca ctggtcgcag
    18121 tgccaataac tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc
    18181 ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa
    18241 gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga
    18301 caatgcgttt cgtcaggcca gtaagcacct tgcccgggcc gacttcatag agatgttcta
    18361 cgccttgcgc tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac
    18421 gtaccagtgc gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca
    18481 caacagaaac tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg
    18541 ctgctggttt catcagcgca cagtgagacg gtacgctcac tggtaacggc agcgcgcgtt
    18601 ttgcgccagc agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa
    18661 taaccacctg tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt
    18721 cttcacacgc tttcgcaata gacgcatcat ccagaccgat gattgccgcc atagcgccag
    18781 tgccttccgg tacggcttct tgcatgaact taccgcgcat ctcaaccaga cgcaccgcat
    18841 cagcgaaatt aatcacgcca gcgcaaacca gggcggagta ttcccccagg ctgtgaccgg
    18901 ccatcattgc cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagatg
    18961 caatcaacag cgctggctgg gtttgccagg ttttattcag ttcttcagct ggcccctgct
    19021 gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga
    19081 caattggata gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag
    19141 ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcgg
    19201 agccccaggt gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc
    19261 gcccgtcgcg tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt
    19321 ggcgatccag cgtcaccacg acattgtcca tagacatacc gagttttttc gccgttgcac
    19381 tgataatacg caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat
    19441 tatttgccgc cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga
    19501 cttcgttgcc cgccatcgtc agatgaattg aattctctgg attcacacgg tcagcattag
    19561 gcagcgtcag caactcacca tagctaccgt cggcatgcag atgggtggag atgattcccg
    19621 gctcttcaga ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc
    19681 cacgatcggt tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact
    19741 tcaccgcccc agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcgc
    19801 aggctgctgc aacgtcaaat gccgggcaac ctttaatgcc caacatgctt tgaatctgac
    19861 aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct
    19921 ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg
    19981 aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc
    20041 actcgtcaga ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca
    20101 gatagctgcc agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata
    20161 taccgtcact tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt
    20221 ttgccaccgt ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt
    20281 cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct
    20341 cgctgattgg ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg
    20401 ccgttatact ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa
    20461 cgctttaata acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga
    20521 gaaaggaaca tcctgacaac accttccatc gtctttaacg tgacatttcc tgtaaagccg
    20581 tcacaaacca gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata
    20641 taattgatag aagggattgt tttaagcacc gctgaggcat cccgaatact gtcgagaccc
    20701 tttacttctt cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc
    20761 tcttcagcca gaactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg
    20821 acgttggccc ctaaatcgag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc
    20881 accagcgccg gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc
    20941 agtgccccgg tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc
    21001 agggccatac gcattgaact cccacgactg gcgcggatag cttgcgaagg ccgggcatca
    21061 ctggcgataa ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct
    21121 ttagcaagta atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa
    21181 ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc
    21241 atgacatcta acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt
    21301 ccccagttgc ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag
    21361 cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt
    21421 caccagaagt tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac
    21481 gcatgccacg tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc
    21541 aattacggtt tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta
    21601 atacggcaaa tgggtttggc ttttgcgctt cttcaggcag ttcaccaaag accatgtccg
    21661 cttcggacac ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga
    21721 tttcatcttc aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt
    21781 catacgcttc cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg
    21841 ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca
    21901 ccttcgcatc gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc
    21961 attccacatc actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg
    22021 tatagatacc ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg
    22081 gtaattttac cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga
    22141 aaaaggcagc ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt
    22201 attttcgttc agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat
    22261 ggctatcatc aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc
    22321 cacctcgcca tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc
    22381 accagaggtc gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact
    22441 ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc
    22501 agaccaggta tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc
    22561 ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt
    22621 gtttaattcg gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg
    22681 tcacctgagc gaggcggaga ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc
    22741 gggtagcttt aagagtgaag gatttggcat tacgctgttt gagcgtttag agggacgtga
    22801 ccctaacacg ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg
    22861 gaaaaacccg ctgatgggat agttgtttgc gccggatgcg gttacgtcgc atccggcaat
    22921 cggtgcaggc ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc
    22981 ttatatgctt gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat
    23041 ccatcggcgc ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg
    23101 cggcgtgcag gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt
    23161 cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac
    23221 ggatctgatg agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc
    23281 gttcttccac tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac
    23341 gttcgccgct ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact
    23401 gccactgacc gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct
    23461 catgcagaga acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc
    23521 ggtcgagacg atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt
    23581 cgataacgcc gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca
    23641 gcaccaggat atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca
    23701 gatgtggcga aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct
    23761 catcacccgc ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcac
    23821 ctttacgcaa aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa
    23881 agttatcgat acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag
    23941 tctctgtttt catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc
    24001 tatgcttata tttagtttta caccttatta cttcactacg tgatcacttt attgatggtt
    24061 attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa
    24121 aagtcatctt gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc
    24181 ttgttaaaac aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc
    24241 aatggcgtaa gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac
    24301 cggtagatgg aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg
    24361 tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg
    24421 tgaggttggc gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt
    24481 tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta
    24541 atcaacgcaa ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat
    24601 gacctggata tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa
    24661 atcacccgca ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac
    24721 ggtttcctcc cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat
    24781 ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat
    24841 aaagaagagc gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc
    24901 tatctggttc tgatgccgaa caacccgcgc gcgggtggca tttctcgccg tatcgaaggc
    24961 gacgaccgta ccgaattaaa agaagcactg gcgagccttg aactgccgga aggcatgggg
    25021 cttatcgtgc gcaccgctgg cgtcggcaaa tctgctgagg cgctgcaatg ggatttaagc
    25081 ttccgtctga aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc
    25141 ctgattcatc aggagagcaa cgtaatcgtt cgcgcattcc gcgattactt acgtcaggac
    25201 atcggcgaaa tccttatcga taacccgaaa gtgctcgaac tggcacgtca gcatatcgct
    25261 gcattaggtc gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg
    25321 ttcagccact accagatcga gtcgcagatc gaatccgcct tccagcgtga agtccgtctg
    25381 ccgtccggtg gttccattgt tatcgacagc accgaagcgt taaccgccat cgacatcaac
    25441 tccgcacgcg cgacccgcgg cggcgatatc gaagaaactg cgtttaacac caacctcgaa
    25501 gctgccgatg agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc
    25561 gacttcatcg acatgacgcc tgtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa
    25621 gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg
    25681 gaaatgtccc gtcagcgcct gagcccatca ctgggtgaat ccagtcatca cgtttgtccg
    25741 cgttgttctg gtactggcac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt
    25801 ctgatcgaag aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg
    25861 ccaatcgctt cttacctgct gaatgaaaaa cgttctgcgg taaatgccat tgaaactcgt
    25921 caggacggtg tgcgctgtgt aattgtgcca aacgatcaga tggaaacccc gcactaccac
    25981 gtgctgcgcg tgcgtaaagg ggaagaaacc ccaaccttaa gctacatgct gccgaagctg
    26041 catgaagaag cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtccggaa
    26101 caacctgcgc tggcaacctt tgccatgccg gatgtgccgc cagcgccaac gccagctgaa
    26161 cctgccgcgc ctgttgtagc cccagcaccg aaagctgcac cggcaacacc agcaactcct
    26221 gcacaacctg ggctgttgag ccgcttcttc ggcgcactga aagcgctgtt cagcggtagt
    26281 gaagaaacca aaccgaccga gcaaccagca ccgaaagcag aagcgaaacc ggaacgtcaa
    26341 caggatcgtc gcaagcctcg tcagaacaac cgccgtgacc gtaatgagcg ccgcgacacc
    26401 cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag
    26461 gcacagcagc agactgccga gacgcgtgag agccgtcagc aagctgaggt aacggaaaaa
    26521 gcacgtacca ccgacgagca gcaagcgccg cgtcgtgaac gtagccgccg ccgtaacgat
    26581 gataaacgtc aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag
    26641 gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc
    26701 aatcagaaag tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt
    26761 gaagaaactg ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg
    26821 gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat
    26881 gctgataacc gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg
    26941 cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca
    27001 atgccgttga ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt ctggatccgc
    27061 tatccaattg tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta
    27121 caagtgcagc cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc
    27181 gcgccagttg ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg
    27241 caaccggaag tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag
    27301 ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa
    27361 ccggtggttg aaccgcagga agagacggca gatattgaag aagttgccga aactgctgag
    27421 gttgtggttg ctgaacctga agttgttgct caacctgccg cgccagtcgt cgctgaagtc
    27481 gcagcagaag ttgaaacggt agccgcggtt gaaccagaga tcaccgttga gcataaccac
    27541 gctaccgcgc caatgacgcg cgctccggca ccggaatatg ttccggaggc accgcgtcac
    27601 agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg
    27661 gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta ataattagct
    27721 caaagtaatc aagccctggt aactgccagg gcttttttat ttcatctttg aatcaacata
    27781 ttaaaattca tcaaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaatgcc
    27841 cgccccggcg atcccagagc aggcagacaa aacataccca gtttcatgat atgttttaaa
    27901 gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa atgctttata
    27961 cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat tccagtccac
    28021 atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg cgatcgctac ctaatgaatc
    28081 cagcgactcc agttcgttca gctgcgtgcc taattccgcg cgaactgtaa ggacattatt
    28141 cagcgagttt ttcagtccac ggttggtttt atccaatgcc gcagcggcga tttctttatc
    28201 cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa
    28261 aagattggtt tcagaagcgc taccgtctgg ttccgctacc gcgttactgg taatactgtc
    28321 gaaaattttg tcgcccgtat gtcctatcac catcgaacgc gaagcatcga cctgttgcct
    28381 aatactttct gctccaccga cgtatttccc tttctcttcg ctaaacggcg cagtctctgt
    28441 tttataaccg gcaaaaatgt agcgcccgtt accgtcagtg gtgttcgcca gattcagcaa
    28501 ctggtcacga agcccctgga tatccgtagc cagcgaggcc cggtcatcgt cactcaaagt
    28561 gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg tgacctggct
    28621 aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgcgcca gcgtgtactg
    28681 gctgttttgc gcctgtgcct gggagagaac aaccgcttgt gatgctgcaa tgggatcgtc
    28741 agaagggtta acgactcgct tacctgtcga catctgctcg ccgtacttca tccattccgc
    28801 ctgagaattg gtgatgccac gcatgttttg ctggtacatc atctgggtac tgaaacgcat
    28861 cttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga
    28921 acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta ctcctcatcg
    28981 agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac attaccttgc
    29041 gtggcgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac taacgaggca
    29101 taagcgtcgt taaaggattt cgcaccgccc accgttttac tgttgctttg cagatccagc
    29161 agggcctgac cgttgcggtt atcgctatca cccgcatctt cttcgctcgc catcgctatt
    29221 ttcgcttcgt cggtgattaa tacatccatg ttgacgatag cgtcacttac tggtttcagc
    29281 gtgaagctgt cgttaacagc aggcgttcct gtaaacgtca actccagacc atcaaatgcc
    29341 actttaccgt tggcatccgg cgtcaccgta aaagtggtat tactggcaag gcgggtgacc
    29401 tgccactgat tattatcgaa cgagattttg taatcagtcg ccagtaccgc ggaggcatca
    29461 gttaccgtgg caccgatcgc aacgtcacct gtgtttttcg tgttttgcag aaccgcgggc
    29521 ttaccgatag caaagaaatc ttcaccagca tcgccgttgg catcaaatcc ggctttgtgt
    29581 tggctgttga aagcctcggc aaatgccagc gccagttgtc caagcgtatt acgcgtctgg
    29641 tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt cagtaatttc
    29701 tccgggatct caatattgcc tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg
    29761 tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa
    29821 ccattggcca tcgtgatgtt ataagtaccg ccatcctgaa cgctgacttc tacgccaaca
    29881 atctggttta attcgctcac cagttgatcg cgttgatcca gcagattgtt aggtgacgcc
    29941 cctgccccca cgcctgtcag gcgcgagatt tgatcgttca ggctggcaat ttgtttagcg
    30001 taattgttga tctgatcaac gctggcacct atcgcgatat tgacctgttt gtcctggtcg
    30061 cgcagatatt gatcggtggt tttaaactga ttcaccaacc cttctgattt cccaatcagc
    30121 gcctggcgcg ctgccgggtc ttccgcgtta ctcaccagcg tttgcaggct ggtgaagaaa
    30181 tcctgcatct gtgttgccag cgaagaggta ctggtggaga gcatattgtc gattttcgac
    30241 atctgctcat agcgggcagt caggccgcta ctttgcgtct gcgccgcacg taactggttg
    30301 gtaataaaag catcatactc acgctgcaca ccagaaacgt agacaccatt gccaacccag
    30361 ccgccagcgc ccaacgtgct attggcctgc gccataatag tggtttggcg ggtatatccg
    30421 gcaacgttat agctggagat attattactt gccgtattta acgccgcctg ggccgcgttc
    30481 agtccgctca tggcgttatt gatcaagctg gacatggagg ttccttataa gccttcaatt
    30541 acagagtatt atcggcagcg acccgccgga cttgagttat tcagaacaga ttatcaatgt
    30601 tcatgctgta ggttttgctc accttgtcgc ttatcgattt catctgctgg atcatgttgg
    30661 tgagtttacg ggcatagtga ggatcggtag cataccccgc gtcctgtagg gcctgcgccc
    30721 cctgctccgc actcgcggcg gtcgtcacgg cggcgtagcg cgggttacgt gttaacagcc
    30781 caacgtaatc cgacaatgct tccagatacg agctgtagac ccgaaacttc gcttttactt
    30841 tcttcgcttc gccgttttca tattcagtcg tggtgatttc agtaactggc cctttccagt
    30901 tgccagaggc tttgacacca aacaggttat agctcggctc gccgttttcg cggcggattt
    30961 gccgttgccc ccaaccagat tccagtgccg cctgagcgag gatcaaatga tgtggcacac
    31021 cgctttgctg gcttgccagt tgggcgggca gcgagagttg cgcgaggaat gctttactgt
    31081 cacccggcag cgaatcatcg tagttacgtg gcacggcctt ttgcaccagc tgcgaaagcg
    31141 cctgattttg ataacgcacc acagtttcga gcgggaattt catcggtgct gctggcgtgg
    31201 actcctctgg caatggttgt tctggcgtca tctgtttaac catcatctct gcaagcccca
    31261 gacctttgcc cgccgtcatc tgttgggcaa tctgctggtc atacatactg gtatacaggc
    31321 gagtgtgctc gctgctgaac aggccatctt ttggtaaagc gtcgcgcatg cttttcaaca
    31381 tcatctgcac gaacatccct tccacctgac gggccaccgg acggatattt gccgccggat
    31441 cttcgcccgc tttcgccttt agttcgttga gtgattgcgc atcccaggcc gcacttgcca
    31501 gtagtttgct gtcgctgatc atcagatgat ttccagtttt gcccgcagac atcccgcact
    31561 ttgcattgat tgcagtatgg acatcagatc catcggcgta gcgcccagcg cattgagcgc
    31621 gcgcaccacg ttattgaggc tggcgctgga acgtacgctt tgcagcgaac cgccgctctg
    31681 gcgtaaatcg atctgcgttt gtggagtaac cacagtctgt ccaccaccaa acggtgtatc
    31741 tggctggctg acattggcct gacgattgac tgttactgag agatttccct gcgctaccgc
    31801 gcagctgtcg agggtcactt cgcgattcat caccaccgaa ccggtgcgcg agttaatcac
    31861 tactttagcg tcctgcgggg tgacattaac ctgcatattc tggatatctg caaggaaacg
    31921 gacctgggaa ctgttgccac ttggtacgcg cacctgaata gtccgcgcat ctaacgcggt
    31981 ggcgctgcca tatccacgca cgcggttgat ggtgtcagcg atttgctgcg ccatgctgaa
    32041 atcttcgtcg ttaagttgca aattaagggt attcccgacg ccaaactggc tgggcaattc
    32101 acgttcaata accgcaccat tggtgatccg tccaccgttc agttggttca cctgaacact
    32161 gctaccgcca gcggaggctc ctgcgccgcc aaccagaata ttgccctgcg ccagcgcata
    32221 cacctgactg tcaacgccct taagcggcgt catcaacaac gtacctccac gcaagctttt
    32281 ggcatttccc atggaagaaa ccaccacatc gatggtttgc ccctgacgtc caaacggagg
    32341 aagtgacgct gtcaccatta ccgcagcgac gttttttagc tgcatattgg tgcccgtcgg
    32401 aacggtaatt cccagctgtg agagcatgtt attaagcgtt tgtgtggtaa acggcgtctg
    32461 ggttgtctgg tcaccggtgc catccagccc caccaccaga ccatagccaa tcagtgagtt
    32521 ttgccttacc ccctgaacac tggtgagatc gcgaatacgc tcagcctgag ccgccgtcgt
    32581 aaccagtaga agaattaatg cagagagaaa tttaatcacc acagcctcac ttacattggc
    32641 gacaggttaa ggaagaaacg ctgcaaccag cccatatttt gcgcttcgtt aatgtagcca
    32701 ttgcctacgt attcaatgcg cgcatccgcc acctgagtag acggtacggt attgctgccg
    32761 ctgatagtgc gtggattaac cacgccagag aagcgaataa attcggtacc ctgattaatg
    32821 gcaatctgtt tttcacccac cacatgcagg ttgccgttga ccagtacctg gtcaaccgtc
    32881 accgtcaacg tgccgctaaa ggtattgctg gcattggccc cgccctttcc gttgaacgtg
    32941 ttaccaccgg aggcttcgac atcggcacga gcgttaccaa acagcccctg caaatagcgc
    33001 ggcacagtat caaagccaaa attagtttta ccgtcacggc tggcattcgc agaggagctt
    33061 ttgctggcgc tgacgttctc ctgcaacacg atggtcagcg tatcgccaat attgcgtggt
    33121 cgacgatctt caaacagcgg ttgatagcca tagttaatcg gctgagcaga ctggaaaata
    33181 gaaccgttgg cgacgggcgt cggcccggga accggttgtg cactggtcgc cccctgcacc
    33241 agcggcgtgg agggtatcca ggcgcagccg gttagtgaaa gcaccaacaa gctggaaatg
    33301 gcataagtat gcgcagcgtt tttttgcatg gctatcatct tcaaaaatca gtaaaccggt
    33361 gaacctgcca ccggttaagc cttagagttg cgtcagtttt tgcagcatct gatcggtggt
    33421 ggacaccgct ttactgttga tttcgtaagc gcgttgcacc tgaatcatat tgaccagttc
    33481 ttccgccacg ttgacgttag acgtttcaac atacccttga tacagcagtc ccgcgccgtt
    33541 caggcccggc gtgctttcgt tcggtgcacc agaggattgc gtttcggtgt agaggttttc
    33601 gccaatgctc tccagcccgg tgtcattcat aaaggtggtg agattgagct gcccaacctg
    33661 aaccggagct gcctggcctt gttgggttac gctgaccacg ccatcacgac cgatggtgat
    33721 acttaacgca ttcgccggaa tggtgatcgc tggctgcacc tgaaaaccac cagccgtcac
    33781 cagctgcccg ttctgatcca cctggaaaga gccgtcacgg gtataggctg atgaaccatc
    33841 tggcaacatc acctggaaaa agccctgccc tttaatcgcg acatctttgc tgttgttggt
    33901 ctgcgacagg tttccctggc tgtgtaagcg ttcagtggcg accgggcgta cccccgtgcc
    33961 gatttgtaat ccggagggta aggtggtttg ttcggaagac tgtgcccccg gctggcgaat
    34021 ggtttgataa agcagatctt caaacaccgc gcgctgacgc ttaaaaccgt tagtactgac
    34081 gtttgccagg ttgttggcaa tgacgtccat attggtttgc tgggcgtcaa ggcccgtttt
    34141 ggcgatccat aatgaactga tcataaaatg tcctgtagtg gattagtgac aagcagagca
    34201 gaagcacgcg acagtgtgaa tttcccctca ccctaaccct ctccccaaag gggcgaggga
    34261 atagtccgcg cgcttttatt gcaatgtcat caatcgttca attaactcat cgacagcagt
    34321 tggttggcac ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg catttcaaaa
    34381 cgccgcgcgc tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt actgccttcc
    34441 agaacccccg acatcacacg caaggttgga tctgcctgca gtatcggccc acgcgtggcc
    34501 tggctttctg cgcttaaacg aaaaatgccg tcgtcaccgc gttgcacttc gctgcccgtg
    34561 gctttcacca gtttcagacg ccccactggc gcaaccgtat ttgccggatc gcccggattg
    34621 agcgccgaga ttgtgccatc ggcagcaata gtgatttccg ccccttccgg cacagcaatt
    34681 ggcccagcct cgcctatcac cggatgcccc tgaattgtca gttgcccggt gggatcaacc
    34741 tgaatgctgc cattacgcgt atacccttcg ctgccgtcag cggactgcac ggccagccag
    34801 ccatcctgct gcaatgcaac gtccagcggg cgcgaggtgt aatccatctt gccgggcgtc
    34861 atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga aagcccttcc
    34921 accggcactg cacgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga ggcattggcc
    34981 agattactgg cggttaccgc ctgttgattc agcgtctggc tggctgctcc catcgcggta
    35041 taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa ccagcgtgtt
    35101 gaggatctgg tcctgggttt tgatggtctg ggcgttagac tgatagttac gctgggcaac
    35161 gatcatattg accagttctt tactgagatc gacgttggac gcttccaacg caccgttggt
    35221 cagggtgcca aagttgcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt
    35281 cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag
    35341 tacaatctgc cccagcagtt gtgtttgttc gttggaatag ttgccgacaa ccgtaccgtc
    35401 atcattgatt tgataactca ccagatcgcc cggtttgtag ccattctggg tggttgccac
    35461 aatattgtta gcgccggtat tttgctgcat ggagttgagg aagctcaggc taaacgtggc
    35521 gggatctgcg ccgttaattg cgccagtggt aatattctct gttggatttg aggtcagaac
    35581 gccattggca ttaaacacca gcttcattgc aggctcggct gtacctgttg gatcactgct
    35641 atcctgggtg tagacctgcc agttattatc cccggtcttc acaaagtaga cgctcatgtc
    35701 atgagcatta ccctgactgt cgaaaacagt caccgaacct tttttgttat agctatccgc
    35761 attgctggcg ctgaatggcg taacagtagg aagcggatca ctggaattca ggttgatctg
    35821 catcgatgcc gtggtggtag ttttcgctgc catcagggta ttcgggatcg aaatattggt
    35881 cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac ccgtcagctg
    35941 taaaccttgc atattcacca ggttacggtt ttcatccagc ttaaattgtc cgttacggct
    36001 gtagaacacc gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc tgatagcaac
    36061 gtccagacct cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccggc
    36121 aacttttacc cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc
    36181 tgatttaaag ccgtaggtgg cggagttggc gatattgttg ccaataacat cgaggttggt
    36241 ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt
    36301 gaaggcttaa attatctgcc gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc
    36361 cagcgtatta ccgctgttgc cgcggatcac accctgcacc agagcaaact gcagcggctg
    36421 ggcaaccagt tgtgtaccac cgttactggc gctaatcgct acattgtaag aaccgttcgg
    36481 cgcagtgctg ccgtcagtca acgtaccgtc ccaggtgaaa ctgtgaactc cagcggtcag
    36541 ttcaccaata tcaatggtgc gcacaaccgc accattttta tcggtgatgg tggccgtaac
    36601 tttgtctgcc gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca cagctccttc
    36661 ttcactgccg gttcccgcaa gaacagtggt gccggggatc atcacgccgt gaccgatcag
    36721 gttactggcc tgtaacgact ggctgttatc aatctgtccg gaaatagatc cgagtgtggt
    36781 attgagtttt tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt
    36841 gttttccatt ggattggtcg ggtcctggtt tttcagctgc gccaccagca aagtcagaaa
    36901 actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac
    36961 gccggtattt gtcggatcgg tggtggttac cgcaatggac atagctttct cctttattga
    37021 ccgagcgtaa gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacgttggcc
    37081 tgatagctgc gtgacgccga catggtgtta accatctccc cgacgacatc aacgttcggc
    37141 atttttacgt agccctttgc atctgccagc ggattacccg gttcataaac cagtttgtcc
    37201 ggggcctgac tttctataac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca
    37261 gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcagg accggtcacg
    37321 ctatcagcat ttgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac
    37381 gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa
    37441 acgttcatca tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg
    37501 ctgttatcgg caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa
    37561 ggctggtccg gaatacggta ttgcagttct gcggtaggag gcgtcagcgc ctgcgccggg
    37621 atgtgttgcg ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc
    37681 atgacttttt taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg
    37741 gcattggcga tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct
    37801 tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa
    37861 ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg
    37921 gtcgctattt atgccgttga tggtcattgc ggacaggtac aattcacgtt gtagaaatgg
    37981 ctgggggcga aaatgctgac aataaaacgt agcgtggcga tcatcgcgat actgttcagt
    38041 ccgttaagta cggcgagcaa tctcacatcg caattgcaca acttttttag cgcccaactc
    38101 gcgggggtaa gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgcca
    38161 tgcgagcagc cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg
    38221 ttggcacgct gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat
    38281 tatgtggttg ccgcgatgcc cattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa
    38341 ctaaaacgcg gacggctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt
    38401 gttgatgcca ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt
    38461 cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg
    38521 tttagcgcca acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg
    38581 gtgcgcatgg tatcgggaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt
    38641 ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat
    38701 aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagcct
    38761 gtaagcaccg ttcaaccgcg cgaaaccact gacgcgccgg taacgaacag ccgggcggca
    38821 aaaacaaccg cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg
    38881 caacccggca gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt
    38941 aacggtgaac taaaaatgga caccggcaaa attgccgatg cgctgatcaa cgaagcgcag
    39001 caagacttgc agagtaactg atcgtatgac acgtcttgca gagatcctcg accagatgtc
    39061 cgctgtgctt aacgatctca aaacggtaat ggatcaagag cagcaacatc tctctatggg
    39121 gcagatcaac ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcgac
    39181 gctggattac ctcgaacagt tacgcaagaa agaacccaat acagcaaata gcgttgatat
    39241 tagtcaacgc tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca
    39301 taacggctgg ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa
    39361 accgcatcag gaaccgacgc tatatggggc gaacggtcag acctcaacaa cccatcgcgg
    39421 cggtaaaaag atttcgatct gaatattatc tccggcctgc actgcaggcc ggaatgcatt
    39481 gttacaccgt ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga
    39541 agtacgcggc aatccccgcc agcacgaccg ccattaaacg cagtaagcgc cagggcatgg
    39601 tacccaatga ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca
    39661 gtaccgccat caccagacgc aacagaaacg ccatccagcc gggttgcgga gtaaagattt
    39721 tctgcttacg caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag
    39781 aaagtgacag cccggcatgt ttcaacggac caataaacgc caggttcatc aattgcgtta
    39841 aaatcagcgt aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc
    39901 caggagccaa cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg
    39961 cccgctgggt catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg
    40021 ttaacggacc agaaagaatg cccaacgcaa ccgcactcgg cagcgccaac aggaaacaaa
    40081 gacgcaaccc ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt
    40141 tcgacagcga cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gacggaaact
    40201 ccattaagcg gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga aacgaggcaa
    40261 aaatggtgtt gatgattaag gagatctggc tcacagagac gccaaggatc gccggtccca
    40321 tctgtttcac cacgcgcatt gttccggcat cgtggaagtt aatgcgcggc aggaccagca
    40381 tgccgatctt cttcaggtgc ggtagctgat acaccagctg caggatgccg ccgaccgtaa
    40441 cagcccacgc cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac
    40501 caatcatgct gatgttaagc agtgttggag caaacgccgg aatcgagaag cggttccacg
    40561 tattcagaat cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa
    40621 tctttagtag ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg
    40681 tcaccatgat cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcga
    40741 gtgtcagcag gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac
    40801 ttttatattc cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac
    40861 ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc
    40921 caaagattct ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca
    40981 tcgagctgac ggcggccagc gattttaata aattcatcgg tgttctaatc cagacccacg
    41041 ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg attatggtga
    41101 attgctaccg ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca
    41161 acgatacgtt gcgccagcac ggcctgttcg ccggcggttt gcggaactgt ctgattttgc
    41221 acacattcaa tgaagtgccg cgcacagccg acaaatccac gttgttcaag agtgctctgc
    41281 caaccaggaa tcggtttatg cactacgccc tgcccgcgct cctcacgcca ttcgcgcata
    41341 tccgtaatgt cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctgacttccg
    41401 gcacggcgat gcatacaggt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca
    41461 aacagcattt cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg
    41521 cccgacaacc acagcacggt atccaccaca tgcagataat catccagcaa cgtgaaataa
    41581 agatcgtgtg gcccgacact attgctacga tgtttatcca ttcttagcga agctgcggta
    41641 gcgagttgcg tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc
    41701 aacgtcagtt ttttacgcgc cgccagttcc accagccgtt cagcatcgcg cagattttct
    41761 gccagcggtt tatcgacaca gacatgtaca cccgcattga gtaacgtact gaccacgtca
    41821 aagtggctgg cggtgctgga atgcacaaaa accgcatcgc aactggcggc aaggctggat
    41881 aacgaatcgg cataaggaat gcgccagctt tcacaaattg gcagggcttt cgcgcgcgta
    41941 ggcgaccagg ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgct
    42001 ttttgcgcaa tgccacctaa tcccactacg ccgatacgta attttttcac tttaatctcc
    42061 cagatgggcc agcaacgaat caagacgctg tttcagttct gccacttcga tttccagggc
    42121 ttcgacgcgg gcctgtaaat caccatcaac cgcgttcgac atatccgtca ccgccggctg
    42181 atcttcaacc tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg
    42241 ggccagacgc accacaaaag gaccatcttc gcgatttgcc agttgttcca gcgtcgactc
    42301 cacttccgcc atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacctgg
    42361 cgtctgggca ccacgcaata acaacgtggt gattaacgcc acttccgctg cgctcagttt
    42421 cagatcgcca aattctgaat tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa
    42481 accgctcact gtgcgtagat aatgacgttt gaccagatta tccagctgtt cctgcacttc
    42541 ggattcactc agattcatga ccggttcacg gttcgttttc tgattacagg ccgtgactac
    42601 accattgact gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc
    42661 aatcacgcga gcttcaaggg cagtaagttg atatttcatg agattctcct tagcggccgg
    42721 gcgtccagtc tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca
    42781 acagatagtc tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc
    42841 tgcgtttatt gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca
    42901 tataacgaat ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc
    42961 caatcgaata accgagatag caggcatgaa aagagccacg aacaacattg gaaaaattgg
    43021 caacgccaat aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt
    43081 gtttatgaaa ttcgttaatc atccccagcc tggcctgcca gcctgatgga taacagtggc
    43141 tttcgtcgcg cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg
    43201 caagacgcca ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc
    43261 gcacttttgg cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt
    43321 ctttaatacg ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccaaaa
    43381 tgccattttc tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga
    43441 taaaaaaata ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag
    43501 tgctcatctt ttatttcccg ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg
    43561 gtaaatattt cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc
    43621 cgctgatctc tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg
    43681 ctctcggtct acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg
    43741 accgctttgg tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg
    43801 ccacaatggg tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac
    43861 tcggcggcac gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac
    43921 aacaacgtgg tcgttttttc tcgctgttga tgatgcagga cagtgccggt gcggtcattg
    43981 gcgcattgtt ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg
    44041 ccgttttgtt tgtgctgtgt gcggcgttca atgcgtggtt gttaccggca tggaaactct
    44101 ccaccgtacg cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg
    44161 tcacctatgt cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc
    44221 tgccaattat ggtcaacgac gtggctggcg caccctctgc cgttaaatgg atgtatgcca
    44281 ttgaagcgtg tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt
    44341 ttcgtctgga acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg
    44401 tgggcatggt cagcggcctg caacaacttt tcaccctgat ttgtctgttt tatatcgggt
    44461 cgatcattgc cgagcctgcg cgtgaaacct taagtgcttc gctggcggac gcaagagctc
    44521 gcggcagcta tatggggttt agccgtctgg gtctggcgat tggcggcgct attggttata
    44581 tcggtggtgg ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga
    44641 tgatgctggg cattattggc atcttcactt tccttgcgct gggttggcag tttagccaga
    44701 aacgcgccgc gcgtcgtttg cttgaacgcg acgcctgatt tgatactccc ccctgactcc
    44761 tccatactga aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg
    44821 atcactgccc ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg
    44881 aattacatgt tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac
    44941 aggttcccat tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gacatcgttc
    45001 actatgtcga taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg
    45061 aagagtggct gcgcaaggtc aatggctacg caaacaaact gctgttgccg cgttttgcca
    45121 aatcggcatt tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag
    45181 aggccagcgc gggtaattta gccgacctgc tggcccactc tgacggtctg attaagaata
    45241 tcagcgatga tttgcgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg
    45301 aactttcgga agatgatatt cagctatttc cgctactgcg taatctgacg ctggtagccg
    45361 gaatcaactg gccaagccgc gttgctgatt accgcgataa tatggcgaaa cagacacaaa
    45421 tcaatttgtt atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg
    45481 ggttgccgcc gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg
    45541 ttgtgtcagg atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt
    45601 gccgctgcat tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc
    45661 atcaccgaac aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc
    45721 ggtttacccg gcgtggctga cgcccatatt gttctgacaa acttgaccag ccaaattggt
    45781 cgcgaagagc cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg
    45841 ttcggtagtc agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa
    45901 gaaaaaggtg cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa
    45961 aaaatgcaaa cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac
    46021 tttaaccagc aacctgctta cgtcctgcgc gaagatggca gtcagggcga agcaatggcg
    46081 aaaaaactgg cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat
    46141 taatgacgag ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta
    46201 tcctttgtgt ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca
    46261 tcccaggtat taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac
    46321 atgttaaaaa ctgtcgtgcc gtataccagc gaaatttatg gacgggctat tgtaatgccc
    46381 aatctggctc cgcccgtgac cactgttgag gctgccgtgg cgtatcgcca gcgtattctt
    46441 gatgccgtac ctgccgggca caatttcacc ccattgatga cctgttattt aacagattcg
    46501 ctggatccta atgagctgga gcgcggattt aacgaaggcg tgttcaccgc tgcaaaactt
    46561 tatccggcaa acgcaaccac taactccagc cacggcgtga cgtcaattga cgcaatcatg
    46621 ccggtacttg agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca
    46681 catgcagata tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct
    46741 ctgcgccagc gcctgactgc gctgaaagtc gtttttgagc acatcaccac caaagatgct
    46801 gccgactatg tccgtgacgg aaatgaacgg ctggctgcca ccatcactcc gcagcatctg
    46861 atgtttaacc gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc
    46921 atcctcaaac gcaatattca ccaacaggca ttgcgtgaac tggtcgccag cggttttaat
    46981 cgagtattcc tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc
    47041 ggctgcgcgg gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa
    47101 gagatgaatg ctctgcagca ctttgaagca ttctgttctg taaacggccc gcagttctat
    47161 ggcttgccgg tcaacgacac attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa
    47221 agcatcgcac tgactgatga caccctggtg ccattcctcg ccggggaaac ggtacgctgg
    47281 tccgttaaac aataaaaatc gtagcttcct gttgtcatta ggttatttta cctgtataaa
    47341 taaccagtat attcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc
    47401 tccattgcca gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta
    47461 tgcgtttcct gataatgaag gccacgtatc ggtacgttat gccgcagcga ataatttatc
    47521 ggttattggc gcaacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg
    47581 ggaaagcgcc gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg
    47641 gtcgccccgg cttttttatt ttacttacaa attttgagta caaaatttct acacttcctc
    47701 aaaataattg cacaatatg
//
LOCUS       DAZGOB010000022        46653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGOB010000022 DAZGOB010000000
VERSION     DAZGOB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.022"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..493
                     /gene="eutS"
                     /locus_tag="ACHLIF_001320"
     CDS             158..493
                     /gene="eutS"
                     /locus_tag="ACHLIF_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO9605369.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            506..985
                     /gene="eutP"
                     /locus_tag="ACHLIF_001321"
     CDS             506..985
                     /gene="eutP"
                     /locus_tag="ACHLIF_001321"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO9605370.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            960..1661
                     /gene="eutQ"
                     /locus_tag="ACHLIF_001322"
     CDS             960..1661
                     /gene="eutQ"
                     /locus_tag="ACHLIF_001322"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO9605371.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1658..2461
                     /gene="eutT"
                     /locus_tag="ACHLIF_001323"
     CDS             1658..2461
                     /gene="eutT"
                     /locus_tag="ACHLIF_001323"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO9605372.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2458..3474
                     /gene="pta"
                     /locus_tag="ACHLIF_001324"
     CDS             2458..3474
                     /gene="pta"
                     /locus_tag="ACHLIF_001324"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO9605373.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3513..3806
                     /gene="eutM"
                     /locus_tag="ACHLIF_001325"
     CDS             3513..3806
                     /gene="eutM"
                     /locus_tag="ACHLIF_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO9605374.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3913..4200
                     /gene="eutN"
                     /locus_tag="ACHLIF_001326"
     CDS             3913..4200
                     /gene="eutN"
                     /locus_tag="ACHLIF_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO9605375.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4212..5615
                     /locus_tag="ACHLIF_001327"
     CDS             4212..5615
                     /locus_tag="ACHLIF_001327"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO9605376.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5626..6462
                     /gene="eutJ"
                     /locus_tag="ACHLIF_001328"
     CDS             5626..6462
                     /gene="eutJ"
                     /locus_tag="ACHLIF_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO9605377.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6452..7639
                     /gene="eutG"
                     /locus_tag="ACHLIF_001329"
     CDS             6452..7639
                     /gene="eutG"
                     /locus_tag="ACHLIF_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO9605378.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7756..8982
                     /gene="eutH"
                     /locus_tag="ACHLIF_001330"
     CDS             7756..8982
                     /gene="eutH"
                     /locus_tag="ACHLIF_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO9605379.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            8979..10382
                     /gene="eutA"
                     /locus_tag="ACHLIF_001331"
     CDS             8979..10382
                     /gene="eutA"
                     /locus_tag="ACHLIF_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO9605380.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLMETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10394..11755
                     /gene="eutB"
                     /locus_tag="ACHLIF_001332"
     CDS             10394..11755
                     /gene="eutB"
                     /locus_tag="ACHLIF_001332"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO9605381.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11776..12663
                     /gene="eutC"
                     /locus_tag="ACHLIF_001333"
     CDS             11776..12663
                     /gene="eutC"
                     /locus_tag="ACHLIF_001333"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO9605382.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12673..13332
                     /gene="eutL"
                     /locus_tag="ACHLIF_001334"
     CDS             12673..13332
                     /gene="eutL"
                     /locus_tag="ACHLIF_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO9605383.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13345..13845
                     /gene="eutK"
                     /locus_tag="ACHLIF_001335"
     CDS             13345..13845
                     /gene="eutK"
                     /locus_tag="ACHLIF_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO9605384.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13891..14943
                     /gene="eutR"
                     /locus_tag="ACHLIF_001336"
     CDS             13891..14943
                     /gene="eutR"
                     /locus_tag="ACHLIF_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO9605385.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHCEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(14949..15848)
                     /gene="hemF"
                     /locus_tag="ACHLIF_001337"
     CDS             complement(14949..15848)
                     /gene="hemF"
                     /locus_tag="ACHLIF_001337"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO9605386.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15852..16721)
                     /gene="amiA"
                     /locus_tag="ACHLIF_001338"
     CDS             complement(15852..16721)
                     /gene="amiA"
                     /locus_tag="ACHLIF_001338"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO9605387.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            16935..17360
                     /locus_tag="ACHLIF_001339"
     CDS             16935..17360
                     /locus_tag="ACHLIF_001339"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO9605388.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17347..17796
                     /locus_tag="ACHLIF_001340"
     CDS             17347..17796
                     /locus_tag="ACHLIF_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO9605389.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            17857..18432
                     /locus_tag="ACHLIF_001341"
     CDS             17857..18432
                     /locus_tag="ACHLIF_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO9605390.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18528..19427
                     /gene="yfeX"
                     /locus_tag="ACHLIF_001342"
     CDS             18528..19427
                     /gene="yfeX"
                     /locus_tag="ACHLIF_001342"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO9605391.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19485..20789)
                     /gene="pbp4b"
                     /locus_tag="ACHLIF_001343"
     CDS             complement(19485..20789)
                     /gene="pbp4b"
                     /locus_tag="ACHLIF_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO9605392.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20794..22218)
                     /gene="murP"
                     /locus_tag="ACHLIF_001344"
     CDS             complement(20794..22218)
                     /gene="murP"
                     /locus_tag="ACHLIF_001344"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO9605393.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22222..23118)
                     /gene="murQ"
                     /locus_tag="ACHLIF_001345"
     CDS             complement(22222..23118)
                     /gene="murQ"
                     /locus_tag="ACHLIF_001345"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO9605394.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23282..24139
                     /gene="murR"
                     /locus_tag="ACHLIF_001346"
     CDS             23282..24139
                     /gene="murR"
                     /locus_tag="ACHLIF_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO9605395.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24268..25059
                     /gene="ucpA"
                     /locus_tag="ACHLIF_001347"
     CDS             24268..25059
                     /gene="ucpA"
                     /locus_tag="ACHLIF_001347"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO9605396.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25230..26246
                     /gene="cysP"
                     /locus_tag="ACHLIF_001348"
     CDS             25230..26246
                     /gene="cysP"
                     /locus_tag="ACHLIF_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO9605397.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26246..27079
                     /gene="cysT"
                     /locus_tag="ACHLIF_001349"
     CDS             26246..27079
                     /gene="cysT"
                     /locus_tag="ACHLIF_001349"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO9605398.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27079..27954
                     /gene="cysW"
                     /locus_tag="ACHLIF_001350"
     CDS             27079..27954
                     /gene="cysW"
                     /locus_tag="ACHLIF_001350"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO9605399.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQDKRAQQEEHHEH"
     gene            27944..29041
                     /gene="cysA"
                     /locus_tag="ACHLIF_001351"
     CDS             27944..29041
                     /gene="cysA"
                     /locus_tag="ACHLIF_001351"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO9605400.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29175..30086
                     /gene="cysM"
                     /locus_tag="ACHLIF_001352"
     CDS             29175..30086
                     /gene="cysM"
                     /locus_tag="ACHLIF_001352"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO9605401.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30089..30457)
                     /locus_tag="ACHLIF_001353"
     CDS             complement(30089..30457)
                     /locus_tag="ACHLIF_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO9605402.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30562..31413
                     /gene="pdxK"
                     /locus_tag="ACHLIF_001354"
                     /gene_synonym="thiJ"
     CDS             30562..31413
                     /gene="pdxK"
                     /locus_tag="ACHLIF_001354"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO9605403.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31455..31964)
                     /gene="crr"
                     /locus_tag="ACHLIF_001355"
     CDS             complement(31455..31964)
                     /gene="crr"
                     /locus_tag="ACHLIF_001355"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO9605404.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32005..33732)
                     /gene="ptsI"
                     /locus_tag="ACHLIF_001356"
     CDS             complement(32005..33732)
                     /gene="ptsI"
                     /locus_tag="ACHLIF_001356"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO9605405.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33777..34034)
                     /gene="ptsH"
                     /locus_tag="ACHLIF_001357"
     CDS             complement(33777..34034)
                     /gene="ptsH"
                     /locus_tag="ACHLIF_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO9605406.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34418..35389)
                     /gene="cysK"
                     /locus_tag="ACHLIF_001358"
     CDS             complement(34418..35389)
                     /gene="cysK"
                     /locus_tag="ACHLIF_001358"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO9605407.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35574..36335)
                     /gene="cysZ"
                     /locus_tag="ACHLIF_001359"
     CDS             complement(35574..36335)
                     /gene="cysZ"
                     /locus_tag="ACHLIF_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO9605408.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36565..37551
                     /gene="zipA"
                     /locus_tag="ACHLIF_001360"
     CDS             36565..37551
                     /gene="zipA"
                     /locus_tag="ACHLIF_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO9605409.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37622..39637
                     /gene="ligA"
                     /locus_tag="ACHLIF_001361"
     CDS             37622..39637
                     /gene="ligA"
                     /locus_tag="ACHLIF_001361"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO9605410.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39639..39857
                     /locus_tag="ACHLIF_001362"
     CDS             39639..39857
                     /locus_tag="ACHLIF_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO9605411.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(39854..40852)
                     /locus_tag="ACHLIF_001363"
     CDS             complement(39854..40852)
                     /locus_tag="ACHLIF_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO9605412.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            40942..41868
                     /locus_tag="ACHLIF_001364"
     CDS             40942..41868
                     /locus_tag="ACHLIF_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9605413.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(41907..42671)
                     /locus_tag="ACHLIF_001365"
     CDS             complement(41907..42671)
                     /locus_tag="ACHLIF_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO9605414.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            42920..43753
                     /gene="xapA"
                     /locus_tag="ACHLIF_001366"
     CDS             42920..43753
                     /gene="xapA"
                     /locus_tag="ACHLIF_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO9605415.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            43813..45069
                     /gene="xapB"
                     /locus_tag="ACHLIF_001367"
     CDS             43813..45069
                     /gene="xapB"
                     /locus_tag="ACHLIF_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO9605416.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            45122..45280
                     /locus_tag="ACHLIF_001368"
     CDS             45122..45280
                     /locus_tag="ACHLIF_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO9605417.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            45321..46205
                     /gene="xapR"
                     /locus_tag="ACHLIF_001369"
     CDS             45321..46205
                     /gene="xapR"
                     /locus_tag="ACHLIF_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO9605418.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(46314..46439)
                     /locus_tag="ACHLIF_001370"
     ncRNA           complement(46314..46439)
                     /ncRNA_class="other"
                     /locus_tag="ACHLIF_001370"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(46471..46546)
                     /locus_tag="ACHLIF_001371"
     tRNA            complement(46471..46546)
                     /locus_tag="ACHLIF_001371"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46511..46513),aa:Lys,seq:ttt)
     gene            complement(46551..46626)
                     /locus_tag="ACHLIF_001372"
     tRNA            complement(46551..46626)
                     /locus_tag="ACHLIF_001372"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46591..46593),aa:Val,seq:tac)
BASE COUNT        10878 a        11808 c        13019 g        10948 t
ORIGIN      
        1 atattcatgc gttgcatatg aaagtttatg caccacagcg aatatctctc cattcttagt
       61 gatctacctc accttttaaa cgcgcttgcc gaattttgtt atttactctg acgaaaaatt
      121 gtcacgatac acgaaagttt ttcacaggcg gcgactcatg gataaagaac gcatcattca
      181 ggaatttgtg cccggcaaac aggtcacact ggcgcatctc atcgcgcatc ccggtgagga
      241 actggcgaag aaaatcggcg ttccggatgc gggcgcaatt ggcattatga cgctaactcc
      301 cggcgaaacg gcgatgatcg ccggtgattt agcgctgaaa gccgctgatg tgcatatcgg
      361 ttttctcgac cgcttcagcg gtgcattggt gatttatggc tcggtgggcg cagtagaaga
      421 agcgttatca caaaccgtca gcggtttagg tcgcttatta aattatacgc tttgtgaaat
      481 gaccaaaagt taatacgagg cattcatgaa acgaattgcg tttgtgggta ccgtcggggc
      541 aggaaaaaca acgttattta atgcgttgca ggggaattat accctcgcca gaaaaacaca
      601 ggccgtggaa tttaatgata agggcgatat tgatacgccg ggtgaatatt ttagccatcc
      661 ccgctggtat cacgccttaa ttaccacgct acaggatgta gacatgctga tctatgtcca
      721 tggcgcaaat gacccggaaa gtcggttgcc tgccgggttg ctggatattg gcgtcagcaa
      781 acggcaaatc gccgtcatca gtaaaacgga catgccagat gctgacgtcg ccgcaacgcg
      841 aaagttattg cttgagacgg gtttcgaaga gccgattttc gagctaaaca gccacgaccc
      901 gcaaagcgtg cagcaactgg tggattatct ggcatctctc accaaacagg aggaagcagg
      961 tgaaaaaact catcacagcg aatgatattc gtgaagcaca cgcgcgcggc gaacaggcaa
     1021 tgtccgtggt tctgcgcgcc agcattatta ccccggaagc ccgtgaagtg gcggatctac
     1081 tgggttttac catcaccgaa tgtgatgaat cgatcccggt aacggcgtcc gtgcccgcca
     1141 gcgtgccagc cgacaaaacc gaaagccagc gcatccgcga aaccatcatt gcccagctgc
     1201 cggaaggcca gtttaccgaa agcctggtcg cacagctgat ggaaaaagtg atgaaggaaa
     1261 aacagtcgct ggagcagggc gcaatgcagc cgagcttcaa atcggtgacc ggcaaaggcg
     1321 gcatcaaagt gattgatggc agcagcgtca agtttggtcg tttcgacggc gcagagccgc
     1381 attgcgtggg cttgactgat cttgtgaccg gagacgacgg cagcagcatg gccgctggtt
     1441 ttatgcagtg ggagaacgca ttcttcccgt ggaccctgaa ctacgacgaa atcgacatgg
     1501 tgctggaagg ggagctgcat gttcgccacg aaggcgaaac catgatcgcc aaagcgggtg
     1561 acgtgatgtt tatcccgaaa ggctccagta tcgaatttgg tacgacatcc agcgtgaaat
     1621 tcctgtatgt cgcctggccg gctaactggc aatccctatg aaagatttca tcaccgaagc
     1681 atggctaaga gcgaaccata cgctcagcga aggcgcagag atccatctcc ctgcggacag
     1741 tcgcctgacg ccctctgccc gggagttact ggaaagccgc catctgcgca tcaagtttat
     1801 tgacgagcag ggccgcctgt ttgttgacga tgaacagcag cagccgcagc ccgttcatgg
     1861 gctgaccagt agcgatgaac atccgcaggc gtgctgcgaa ctgtgccgcc agccggtggc
     1921 aaaaaagccg gatacgctga ctcacctgtc ggcggaaaaa atggtcgcca aaagcgatcc
     1981 gcgtctgggt tttcgcgcgg ttctcgacag caccattgcg ctggcagtgt ggctgcaaat
     2041 tgaactggcg gaaccgtggc agccgtggct ggcggatatc cgttcgcgtc ttggcaacat
     2101 tatgcgcgcc gatgcgctgg gtgaaccgct gggctgccag gcgattgtcg ggctttctga
     2161 cgaagatctg catcgacttt ctcaccagcc gctgcgctat ctcgaccacg atcatctggt
     2221 gccggaagcc agccacgggc gcgatgcggc gttgctcaat ctgctacgta ccaaagtgcg
     2281 ggaaaccgaa accgtagcgg cgcaggtgtt tatcacccgc agctttgaag tattacgccc
     2341 ggacattttg caggcgctga accgcctttc cagcacggtc tacgtgatga tgatcctgag
     2401 cgtaacgaag cagccgctaa cggtgaagca gatccaacaa cgactggggg aaacgcaatg
     2461 attattgaac gttgtcgtga actggcgttg cgagcgcccg ccagagtggt ttttccggat
     2521 gcgttagacc aacgtgtgct gaaagccgcg caatatttac atcaacaagg tctggcaacg
     2581 cccattctgg tcgccaatcc gtttgaactt cgtcagtttg cgctcagtca cggcgtggcg
     2641 atggacgggc tacaggtgat tgatccgcat ggcaacctcg caatgcggga agaatttgct
     2701 catcgctggc tggcccgcgc gggcgaaaaa acgccgccgg atgcgctgga aaaacttacc
     2761 gaccctctga tgttcgccgc agcaatggtc agcgccggta aagcggatgt ctgtatcgcg
     2821 ggcaacctct cttccacggc aaatgtgctg cgtgccggat tacgcattat cggcttgcag
     2881 ccaggctgta aaacgctctc ttccattttc ctgatgttgc cacagtacag cggcccggcg
     2941 ttgggctttg ccgattgcag cgtggtgcca cagccgacgg cggcgcagct ggcggatatc
     3001 gcgcttgcca gtgccgaaac ctggcgcgcc atcaccggag aagagccgcg cgtggcgatg
     3061 ctgtcgtttt ccagcaatgg tagcgcccgt cacccctgtg ttgctaacgt ccagcaggcg
     3121 acagaaatcg tccgtgagcg cgcaccaaag ctggtggtgg atggcgagtt acagtttgac
     3181 gccgccttcg tgccggaagt ggcggcgcaa aaagcgcctg ccagcccgct acagggcaag
     3241 gccaatgtga tggtttttcc gtcgctggaa gccggaaata ttggttacaa aatcgcacaa
     3301 cgactcggcg gatatcgtgc cgtcgggcca ctgatacaag gacttgccgc gccgatgcac
     3361 gatctctctc gtggttgtag cgtgcaggaa attatcgagc tggcgctggt ggcagctgtg
     3421 ccgcgtcaga cagaagtgaa ccgcgaaagc agtttacaaa cactggttga atgaacggtc
     3481 ccgttctgga ccctgataaa gaggaaagca caatggaagc attaggaatg atcgaaaccc
     3541 ggggcctggt tgcactgatt gaggcttctg acgcgatggt gaaagctgcg cgcgtcaagc
     3601 tggttggtgt taagcagatt ggcggcggcc tgtgtactgc catggttcgt ggtgatgttg
     3661 cagcctgtaa agccgcgacc gatgcaggtg ccgcagcggc acagcgtatc ggcgagctgg
     3721 tatctgtaca cgttatcccg cgtccgcacg gcgacctgga agaagtcttc ccgatcggcc
     3781 tgaaaggcga tagcagcaac ctgtaatcac cctgtgtgac gtcgattcgg gcgtcacgct
     3841 ctctcctttt tcattttacc ttctgcggag ggtaggggga gcttttacct gaaatacgcc
     3901 acggaggcgg gtatgaaact ggcagtcgtc actggacaaa ttgtttgtac cgtacgccat
     3961 cacggactgg cgcatgacaa attgctgatg gtggaaatga ttgatccaca aggtaatcct
     4021 gacgggcaat gcgccgtcgc catcgacaat attggcgcgg gaaccgggga gtgggtgtta
     4081 ctggtgagtg gcagttccgc ccgccaggcg cataaaagcg aaacgtcacc ggtcgatctg
     4141 tgcgtgattg gcattgtcga tgaggtggtg tctggcggtc aggtaatttt ccacaaataa
     4201 ggcagaacat catgaatcaa caggatattg aacaggtggt gaaagcggta ctgctgaaaa
     4261 tgcaaagcag tgacacgccg tccgccgccg ttcatgagat gggcgttttc gcgtccctgg
     4321 atgacgccgt tgcggcagcc aaagtcgccc agcaagggtt aaaaagcgtg gcaatgcgcc
     4381 agttagccat tgctgccatt cgtgaagcag gcgaaaaaca cgccagagat ttagcggaac
     4441 ttgccgtcag tgaaaccggc atggggcgcg ttgaagataa atttgcaaaa aacgtcgctc
     4501 aggcgcgcgg cacaccaggc gttgagtgcc tctctccgca agtgctgact ggcgacaacg
     4561 gcctgaccct aattgaaaac gcaccctggg gcgtggtggc ttcggtgacg ccttccacta
     4621 acccggcggc aaccgtaatt aacaacgcca tcagcctgat tgccgcgggc aacagcgtca
     4681 tttttgcccc gcatccggcg gcgaaaaaag tctcccagcg ggcgattacg ctgctcaacc
     4741 aggcgattgt tgccgcaggt gggccggaaa acttactggt tactgtggca aatccggata
     4801 tcgaaaccgc gcaacgcttg ttcaagtttc cgggtatcgg cctgctggtg gtaaccggcg
     4861 gcgaagcggt agtagaagcg gcgcgtaaac acaccaataa acgtctgatt gccgcaggcg
     4921 ctggcaaccc gccggtagtg gtggatgaaa ccgccgacct cgcccgtgcc gctcagtcca
     4981 tcgtcaaagg cgcttctttc gataacaaca tcatttgtgc cgacgaaaag gtactgattg
     5041 ttgttgatag cgtagccgat gaactgatgc gtctgatgga aggccagcac gcggtgaaac
     5101 tgaccgcaga acaggcgcag cagctgcaac cggtgttgct gaaaaatatc gacgagcgcg
     5161 gaaaaggcac cgtcagccgt gactgggttg gtcgcgatgc ggcgaaaatc gcggcggcaa
     5221 tcggcctgaa cgtgccgcaa gaaacgcgtt tgctgtttgt ggaaaccact gcagaacatc
     5281 cgtttgccgt gaccgaactg atgatgccgg tgctgcccgt cgtgcgcgtc gccaacgtgg
     5341 cggatgccat tgcgctggcg gtgaaactgg agggcggttg ccaccacacg gcggcaatgc
     5401 actcgcgcaa tatcgaaaac atgaaccaga tggcgaacgc cattgatacc agcattttcg
     5461 ttaagaacgg accgtgcatt gccgggctgg ggctgggcgg ggaaggctgg accaccatga
     5521 ccatcaccac gccaaccggt gaaggggtaa ccagcgcgcg gacgtttgtc cgtctgcgtc
     5581 gctgtgtatt agtcgatgcg tttcgcattg tttaaggagt aaccgatggc gcacgacgaa
     5641 caatggctca ccccacgtct gcaaacggcg gcaacgctgt gtaaccagac gcctgccgcg
     5701 acggaatctc cgctgtggct gggggtggat ctgggcacct gcgatgtggt gtcgatggtt
     5761 gttgaccgcg acggtcagcc ggtggcggta tgcctcgact gggccgatgt ggtccgcgac
     5821 ggcatcgtct gggatttctt cggtgcggtc accattgttc gtcgccatct cgacactctt
     5881 gagcagcaat tcggtcttcg ttttagccat gcggcgactt catttcctcc aggtacggac
     5941 ccgcgtattt ccatcaacgt gctggaatct gccgggctgg aagtgagcca tgtgctggat
     6001 gagccaaccg cagtcgcgga tctgctgcaa ctggacaacg ccggtgtagt ggatatcggc
     6061 ggcggcacta ccggcattgc catcgtgaaa aagggcaagg tgacgtactc ggcggatgaa
     6121 gccaccggcg gacatcacat ctctctgact ctcgccggaa atcgtcgtat ttcgctggaa
     6181 gaggcagagc agtacaagcg cggtcatggt gacgagattt ggcccgcggt gaaaccggtg
     6241 tacgaaaaaa tggcggacat tgtcgcccgg catattgaag ggcagggcat tactgattta
     6301 tggctggcgg gcggctcttg tatgcaaccg ggcgtggcgg aactgtttcg caaacaattc
     6361 ccggcgttac aggtgcattt accgcagcac agtttgttta tgaccccgct ggcgatcgcc
     6421 agtagtgggc gagagaaagc ggaggggcta tatgcaaaat gaattgcaga ccgcgctctt
     6481 tcaggcgttc gataccctga atctgcaacg ggtaaaaaca tttagcgttc caccggtgac
     6541 gctttgcggt ccgggctcgg tgagcagttg cggacagcaa gcgcaaacgc gtgggctgaa
     6601 acatctgttc gtgatggcag acagcttttt gcatcaggca gggatgaccg ccgggctgac
     6661 gcgtagcctg gccgttaaag gtatcgccat gacgctctgg ccatgtccgg tgggcgaacc
     6721 gtgcatcacc gacgtgtgtg cagccgtggc gcagttgcgt gagtcaggct gtgatggggt
     6781 gatcgcattt ggcggcggct cggtgctgga tgcggcgaaa gccgtggcgt tgctggtgac
     6841 gaacccggat agcacgctgg cagagatgtc agaaaccagc gttctgcaat cgcgcctgcc
     6901 gttgattgcc attcccacta ccgccggaac cggctctgaa accaccaacg taacggtgat
     6961 tatcgatgcg gtgagcgggc gtaagcaggt gttagcccat gcctcgctga tgccggatgt
     7021 ggcgattctc gacgccgcat tgaccgaagg tgtgccgtcg catgtcacgg cgatgaccgg
     7081 cattgatgcg ttaacccatg ccattgaagc atacagcgcc ctgaacgcta caccgtttac
     7141 cgacagtctg gcgattggtg ccattgcgat gattggcaaa tcgctgccga aagcggtggg
     7201 ctacggtcat gaccttgccg cgcgcgagag catgttactg gcttcatgta tggcgggaat
     7261 ggcgttttcc agtgcggggc ttgggttgtg ccacgcgatg gcgcatcagc caggggcggc
     7321 gctgcatatt ccacacggtc tggcgaacgc catgttgctg ccaacggtga tggaatttaa
     7381 ccggatggtt tgtcgcgaac ggtttagtca gattggtcgg gcactgcgaa ctaaaaaatc
     7441 cgacgatcgt gacgctatta acgcggtaag tgagctgatt gcggaagttg ggattggtaa
     7501 acgactgggc gatgttggcg cgacatctgc gcattacggt gcatgggcgc aggccgcgca
     7561 ggaagatatt tgtctgcgca gcaacccgcg taccgccagc ctggagcaga ttgtcggcct
     7621 gtacgcagcg gcgcaataaa tgccggatgc gacgcttgcc gcgtctgatc cggccttcgg
     7681 gattgcacat gtagggcgga taaggcgttt acgccgcatc cgccaataaa taacaactaa
     7741 cagggagtaa aggcgatggg aattaacgaa atcatcatgt acatcatgat gttctttatg
     7801 ctgatagctg ccgtagacag gatcctgtcg cagttcggcg gttctgctcg tttcctcggt
     7861 aagttcggta aaagtatcga aggatcaggc ggtcagttcg aagaaggctt tatggcaatg
     7921 ggcgcactgg gcctggcgat ggtcggtatg accgcgctgg caccggtact ggctcacgta
     7981 ctcgggccgg taattattcc ggtttacgaa atgctcggcg ctaacccatc gatgttcgcc
     8041 ggaacactgc tggcgtgcga tatgggcggc ttcttcctcg ccaaagagct ggcgggcggc
     8101 gacgtagcag cgtggctata ctctgggtta attctcgggt cgatgatggg gccaacgatt
     8161 gtgttttcca ttccggtggc gctcggcatt atcgaacctt ctgaccgtcg ttatctggcg
     8221 ctcggcgtgc tggcgggcat tgtgaccatt ccgattggct gtattgctgg tggtctggtt
     8281 gctatgtact ccggtgtgca gatcaacggt cagccggtgg aattcacttt cgccctgatc
     8341 ctgatgaaca tgatcccggt gatcattgtc gcgattctgg tggcgctggg gctgaaattc
     8401 atcccggaaa aaatgatcaa cggcttccag atcttcgcca aattcctcgt ggcattgatc
     8461 accctcggtc ttgccgctgc ggtagtgaaa ttcctccttg gctgggaact gatccccgga
     8521 ctggatccta tctttatggc ccctggcgat aaacccggtg aagtgatgcg cgccattgaa
     8581 gttatcggtt ctatctcctg cgtgctgtta ggggcgtatc caatggtgct gctgctgact
     8641 cgctggtttg aaaaaccgct gatgagcgtc ggtaaagtgc tgaatatgaa caacatcgcg
     8701 gcagccggca tggtggcaac gcttgccaac aacatcccga tgttcggcat gatgaagcag
     8761 atggataccc gcggcaaagt catcaactgc gccttcgccg tttccgctgc tttcgccctg
     8821 ggcgaccact taggcttcgc cgctgccaac atgaacgcca tgatcttccc gatgattgtc
     8881 ggcaagttga tcggcggcgt aacggcgatt ggcgtggcga tgatgctggt gccaaaagaa
     8941 gacgcgaccg cgactaaaac cgaagcggag gcacaatcgt gaacactcgc cagctattga
     9001 gcgtcggtat cgatatcggc accaccacca cccaggtgat tttctcccac ctggagctgg
     9061 ttaaccgtgc ggcggtgtcg caggtgccgc gctacgaatt cattaaacgc gaaattagct
     9121 ggcaaagtcc ggtgttcttt acccctgtcg ataaacaggg cggtttaaaa gaagcggaac
     9181 tgaaaacctt aatactcgag caatatcagg ctgcgggtat tgcgccggaa agcgttgatt
     9241 ctggtgccat catcatcacc ggtgaaagcg cgaaaacccg caatgctcgc ccggcggtga
     9301 tggcgctctc tcaatcgctg ggggattttg tcgttgccag cgccgggccg cacctcgaat
     9361 ccgtgatcgc cggtcacgga gctggggcgc aaaccctttc tgaacaacgg ctgtgtcggg
     9421 tactgaatat cgacatcggc ggtggcaccg cgaactacgc cctgttcgat gccggaaaaa
     9481 tcagcggcac tgcctgtctc aacgtcggtg gtcgcctgat ggaaaccgac agccaggggc
     9541 gcgtggttta cgctcataaa ccggggcaga tgattgtgga tgagtgcttc ggtgcaggca
     9601 ctgacgcccg ttcgctgacc ggcgcgcagc tggtgcaggt tacccggcgg atggcggcgc
     9661 tgattgtcga agtgattgac ggaacgcttt cgccgctcgc gcaggcattg atgcaaaccg
     9721 gtttgctgcc cgcaggtgtt acgcccgaaa tcattacgct ttctggaggc gtgggcgaat
     9781 gttatcgcca ccagcccgcc gacccgttct gttttgccga tattggccca cttctggcaa
     9841 cggcgctgca tgaccatccg cgcctgcgtg agatgaatgt gcagtttccg gcgcaaaccg
     9901 tacgcgccac ggtgattggc gcgggtgcac ataccctttc gctctctggc agcacaatct
     9961 ggctggaggg cgtacaactg ccgctgcgca atttgccggt ggcgatcccg attgatgaaa
    10021 cggatctggt gagtgcctgg caacaggcgc tgcttcagct ggatcttgat cccaaaactg
    10081 acgcgtacgt gctggcgctt cccgcctcgc tgcctgtgcg ttacgccgcg gtactgacgg
    10141 tcatcaacgc gctggtcgat ttcgtcgcgc gttttccgaa tccgcatccc ctgctggtgg
    10201 tggccgggca ggactttggt aaagctctgg gcatgttgtt gcgcccacag ctacaacaac
    10261 tcccgttggc agtcattgac gaagtgattg tccgcgcggg ggactatatc gacattggta
    10321 cgcctctttt tggcggatcg gttgtgccgg tgacggtgaa atcactcgca tttccttcct
    10381 gagggaacga cttatgaaac taaagaccac attgttcggc aatgtatatc agtttaagga
    10441 tgtaaaagag gtgctggcta aagccaacga actgcgttcg ggggatgtgc tggcgggcgt
    10501 tgcagcggca agctcacagg agcgcgtggc ggcaaagcag gtgttgtcgg aaatgaccgt
    10561 agcggacatc cgcaataatc cggtgattgc ctatgaagat gactgcgtga cgcggctgat
    10621 tcaggacgat gttaacgaaa cggcctacaa ccagattaaa aactggagca tcagcgaact
    10681 gcgtgagtat gtgctgagcg atgaaaccag cgtggacgac attgccttta cccgcaaagg
    10741 gctgacctcg gaagtggtcg cggcggtagc gaagatttgc tccaacgcgg acctgatcta
    10801 cggcgcgaag aaaatgccgg taatcaaaaa ggccaatacc accattggta ttccgggcac
    10861 cttcagcgcc cgtttgcagc cgaacgatac ccgtgacgac gtgcaaagta tcgctgcgca
    10921 aatctatgaa gggctttcct ttggggtggg cgatgcggtg atcggcgtta acccggtaac
    10981 tgacgacgtg gaaaacttaa gccgcgtgtt ggacaccatt tacggcgtga tcgacaaatt
    11041 caacatccca actcagggct gcgtactggc gcacgtcacc acccagatcg aagcgattcg
    11101 tcgcggcgcg cctggcggac tgattttcca gagtatctgt ggcagcgaaa aagggctgaa
    11161 agagtttggc gtggaactgg cgatgctcga cgaagcgcgc gcagtgggtg cagagttcaa
    11221 ccgtatcgcc ggggaaaact gcctctactt cgaaaccgga caaggctctg cgctatccgc
    11281 tggcgctaac ttcggcgctg accaggtgac gatggaagca cgtaactatg ggctggcgcg
    11341 tcattacgat ccgtttatcg tcaacaccgt ggtcggcttt attgggccgg agtatctcta
    11401 caacgaccgc cagattatcc gtgctggctt agaagatcac tttatgggca agctgagcgg
    11461 catctctatg ggctgtgact gctgttacac caaccacgct gacgctgacc agaacctcaa
    11521 cgaaaacctg atgatcctgc tcgccaccgc aggctgcaac tacatcatgg ggatgccgct
    11581 gggtgatgac atcatgctca actaccagac caccgcattc cacgataccg ccactgtgcg
    11641 tcagttactc aacctgcgtc cgtcaccgga gtttgaacgc tggctggaaa gcatgggcat
    11701 tatggcaaac ggtcgcctga ccaaacgggc gggcgatccg tcactgttct tctgatgacg
    11761 cggagataac acatcatgga tcaaaaacag attgaagaaa ttgtacgcag cgtgatggcg
    11821 tcaatgggac aaacggcccc cgcgccgtca gaagcaaagt gcgccaccac caactgtgcg
    11881 gcaccggtga cctcggaaag ctgcgcgctg gatttaggtt ctgcggaagc aaaagcgtgg
    11941 attggtgttg aaaacccgca tcgcgcagac gtattaacag aactgcgtcg cagcaccgtg
    12001 gcccgcgttt gtaccggtcg tgccggtccg cgtccgcgta cgcaggcgtt gctgcgtttc
    12061 ctggccgatc actctcgttc gaaagatacc gtactgaaag aagtgccgga agagtgggtg
    12121 aaagcgcagg gcttgctgga agtacgctcc gaaattagcg acaaaaatct ctacctgact
    12181 cgcccggata tgggccgccg cctgtgtgca gaagctgttg aagcgctgaa agcacagtgt
    12241 gttgctaatc cggacgtaca ggtcgttatt tctgatggcc tgtcaactga tgccatcacc
    12301 gtgaactacg aagagatcct gccgccgctg atggcgggcc tgaaacaggc cgggctgaaa
    12361 gtcggcacgc cattctttgt tcgttatggt cgcgtgaaga ttgaagatca gatcggtgag
    12421 atcctcggcg cgaaagtggt gatcctgctg gtgggggaac gtccggggtt agggcagtca
    12481 gaaagcctct cctgctacgc cgtttactcg ccgcgtatgg cgaccaccgt tgaggccgat
    12541 cgcacctgta tctctaacat tcaccagggc ggcacgccgc cggttgaagc cgcagccgtc
    12601 attgtggatt tggcgaaacg tatgctggag cagaaagcat ccggcatcaa catgacccga
    12661 taaggaggca tcatgccagc tttagatttg attcgaccgt cggtaaccgc catgcgggtg
    12721 attgcctccg ttaacgctga atttgcgcgt gaactaaaat tgccgccgca tattcgtagc
    12781 ctcgggctga tttctgccga ttctgacgac gtcacctaca ttgcggctga cgaagcgacc
    12841 aaacaagcga tggtcgaagt ggtatatggt cgctcgctgt atgcgggagc ggcacacggc
    12901 ccgtcaccga ctgccggtga agtgctgatt atgcttggtg ggccaaaccc ggcggaagtg
    12961 cgtgccggtc tggatgcgat ggttgcacat attgaaaatg gcgcagcttt ccagtgggcc
    13021 aatgacgcag aaaacaccgc cttcctggca catgtagttt cgcgtaccgg ttcttatctc
    13081 tcatcaaccg ccgggatcac gcttggcgat ccgatggcgt atctggtggc accgccgctg
    13141 gaagcgacct acggcattga tgcagcgttg aaatctgccg acgtacagct ggtgacctat
    13201 gtcccgccac cgtctgaaac caactactcg gcagcatttt taaccggtag ccaggccgcg
    13261 tgtaaagcag cctgtaacgc ctttaccgat gcagtgctgg aaatcgcgcg taatccaatc
    13321 cagcgtgcgt aacggaggtt gccgatgatc aatgcactgg gattgctgga agtggacggc
    13381 atggtcgccg cgatagatgc agcggatgcc atgctcaaag cagctaacgt tcgtctgctc
    13441 agtcacgaag tgcttgaccc tggtcgctta acgctggtgg tggaaggcga tctggcggcg
    13501 tgtcgtgcgg cgctggacgc aggttgtgct gccgcgatgc gtaccgggcg tgtcatcagc
    13561 cgcaaggaga tcggtcggcc agacgatgac acccagtggc tggttactgg ctttaaccgc
    13621 cagccgaagc aacccgtaag ggaacccgac gcgccagtta tcgtcgcgga atctgctgac
    13681 gagttgttgg cgctgttaac atcagtacgt cagggaatga cggcaggaga agtggctgcc
    13741 cactttggct ggccgctgga aaaagccaga aatgcgctcg aacagctctt ttctgccggg
    13801 acgttacgta aacgcagtag tcgttatcgt ctcaagcccc attaacctgt cggaggtgcc
    13861 gggtgtccta caacacccgg cattaacatc atgaaaaaga cccgtacagc caatttgcac
    13921 catctttatc atgaaccctt acccgaaaac ctgaagctca cgccgaaggt cgaagtggat
    13981 aatgttcatc aacgacagac aacggatgtc tatgaacatg ctttaacgat taccgcctgg
    14041 cagcagattt acgatcagct gcatccgggc aagtttcatt gtgaatttac ggaaattcta
    14101 ctcgatgata ttcaggtttt tcgtgaatac accggtctgg cgctgcgtca gtcgtgcctg
    14161 gtctggccga actcgttctg gtttggcatt ccggcgacgc gcggtgagca gggatttatc
    14221 ggttcgcaat gtctgggaag cgcggaaatc gccacccgcc ctggtggcac tgaatttgaa
    14281 ctgagcacgc cggatgatta cacgatcctg ggcgtggtgc tttctgaaga tgtcatcacc
    14341 cggcaggcta actttttgca taacccggat cgggtattac atatgctgcg tagccagtcg
    14401 gcgctggaag tgaaagagca gcataaagcc gcgctgtggg gctttgtcca acaggcgctg
    14461 gcgacgtttt gcgagaaccc ggaaaatctc catcagcctg cagtgcgaaa agtgctgggg
    14521 gataatttgc taatggcgat gggggcgatg ctggaagacg cgcaaccaat ggtgacggcg
    14581 gaaagcatca gtcatcagag ttaccgtaga ttactttccc gcgcccgtga atatgtgctg
    14641 gaaaatatgt ctgagccggt gacggtgctg gacttgtgta atcaactgca tgtcagtcgc
    14701 cgcacgctac aaaacgcgtt tcacgctatt ttaggcattg gcccaaacgc gtggctgaaa
    14761 cgcattcgcc tgaacgccgt acgccgcgaa ctgataagcc cgtggtcgca aagcacaacg
    14821 gtaaaagacg ccgccatgca gtggggattc tggcatctgg ggcaatttgc cacggattac
    14881 cagcagctgt ttgccgagaa gccgtcgttg acgttgcatc agcggatgcg ggaatggggg
    14941 tgagggagtt acacccaatc cctgacctta ataaactcac ttaacgccgc ttctgggctg
    15001 ccatcttttg gctgataatc atactcccag cgtaccagtg gcggcattga catcaggatc
    15061 gactcggtgc gcccgccagt ttgcaggcca aacagagtgc cgcgatccca gaccagattg
    15121 aactcgacat aacgaccgcg acggtagagc tgaaaattgc gctcgcgctc gccgtaggcc
    15181 atcgctttac gtcgctctac aattggtaaa taagcgtcgg tgtagccttt gcctaccgcc
    15241 tgcataaagg caaaacagtg gtcgaaatct ggcgtgttca gatcatcaaa gaacagcccg
    15301 ccaataccgc gctgttcgtt gcgatgtttg aggtagaagt aatcgtcgca ccactttttg
    15361 taacggggat aaacgtcttc accaaatggc aggcacaggt cacgggcggt gcggtgccag
    15421 tgaatggcgt cttcttcaaa accatagaaa ggggttaaat cgaagccgcc gccaaaccac
    15481 cagacgggct ctgcacccgg tttttcggca ataaaaaaac gcacattcgc gtggctggtg
    15541 ggaacatacg ggttatgcgg atgcactacc agtgaaacgc ccatcgcctc gaaactgcgc
    15601 ccggcaagtt ccgggcgatg agcggtggcg gaagctggca tcgcctcgcc gtggacgtga
    15661 gaaaagttga cgcctgcctg ttcgaaaaca ccaccattac gcaacacccg actacgcccg
    15721 ccgccgccag cttcgcgctg ccaactatct tcgacaaatt ctgcgccatc gacggcggtc
    15781 agctgctgac aaatcgtatc ctgaaggttg agcagaaact gtttaacctg gtgtgcgtcg
    15841 ggtttcataa cttatcgctt tttcgaatgt gctttctggt tgtcgaacca gtggaaataa
    15901 ctgatcacgc cttcagcaat cgctgtggcg attttctgac gaaacgccgc cgtgcctaac
    15961 agccgctctt cttccgggtt ggtgataaac gaggtttcca ccagcaccga aggaaccgac
    16021 ggcgatttca acaccacaaa cgcggcttgt tcggtgttgc ggctgtgcag tttatgcacc
    16081 ggcttaatct ttttcagaat atgcgagccg agcgtcaggc tgtttttaat agtatcggtt
    16141 tgcaccagat caaacagcac ttgctgcaat aggtgatcct tgtcagtcgc ctttttaccg
    16201 gcaacttcat cggcgcggtt ttcgcgttca gacaggtatt tcgccattgc gctacttgcc
    16261 ccacggttag agagggcaaa taccgaagca ccggcagctt tcgggttggt aaagccatcg
    16321 gcatgaattg acataaacag atctgcgcca tgtttatggg cgatttcaac gcgatcgtaa
    16381 agtgggataa acgtatcgcc agaacgcgtt aaacgcgcat caatcccatg attacgcaaa
    16441 atggaacgga cgtttttagc aatcgccagc accacatgtt tttctttcga accgttgcgt
    16501 ccgatcgctc cggtatcaat tccgccgtgg cctggatcga gaacaacgac acgtttgccg
    16561 ccagattttt tggctttcgg cttgctgtgt ccgttgctgg tttttaaagg ttcgtctttg
    16621 gcgatggctt gcgacattcc tgacaacgtc agggcagcca aaccggcttt cagcacctgg
    16681 cggcgcgaag tgagtgtttt tagtggttta aaagtgctca tacggcctga gttgtaataa
    16741 taaagttcca gatgttatat cgtatcgcgt aatccgttac gacggtttga tgttgagaat
    16801 tgttttactt ttcatttcaa tagttgacaa ctccccatta tgccactttt tcaaccgatt
    16861 ttcgtcagat attggctaat tcgaccgttc acgccataat cagcctttta acccgcaaaa
    16921 aggtaacgaa taccatggag atacgcgtat ttcgccagga agatttcgaa gaggtcatca
    16981 ccctttggga gcgttgcgac ttgctgcgtc cgtggaacga tccggaaatg gacatcgagc
    17041 gtaagatgaa ccatgacgtc agtttgtttt tggtcgctga ggtaaacggt gaagtggtcg
    17101 gaacggtgat gggcggttat gacgggcatc gcgggtctgc ttattatctt ggcgtgcatc
    17161 cagagtttcg tgggcgtggg attgccaatg cgttgcttaa tcggctggag aaaaagctga
    17221 ttgctcgtgg ctgcccgaaa attcagatca atgtgccgga agataacgac atggtgctcg
    17281 gaatgtatga acgcctggga tatgaacacg ccgacgtgct gagtctgggt aagcgtttga
    17341 ttgaagatga agagtactga gttccatcct gtccattatg atgcgcacgg acgcctgcgt
    17401 ttacccttgc tcttctggct tgtgctattg cttcaggcgc gaacctgggt gctgtttgtc
    17461 attgccggtg cgtcgcgcga gcagggtact gcgctgctaa atctgtttta tcccgatcac
    17521 gataatttct ggctggggct gattccaggc attccggcgg tgctggcgtt tttgttgagc
    17581 ggtcggcggg ctacatttcc tcgcacttgg catgtgctct attttctgtt gctgttggcg
    17641 caggtggttt tactttgctg gcaaccgtgg ctgtggctga acggtgaatc cgttagcggt
    17701 atcggtctgg cgctggtagt ggcggatatt gtggcgttaa tctggctgct gaccaatcga
    17761 cgtttacgcg cttgttttaa tgaagaaaaa gaataacggc actttttggt gaatttgcac
    17821 tccaagcaac gttattgaat aaccaaagga agtgacatga aatcgctgcg tttaatgtta
    17881 tgcgctatgc cgttgatgct gaccggctgt tccacgatgt cgtcagttaa ctggtcggcc
    17941 gctaacccgt ggaactggtt tgggtcgtcc accaaagtga gcgagcaggg cgttggtgaa
    18001 ttaacggcgt ccacaccact gcaagaacaa gccattgccg atgcgcttga tggcgattat
    18061 cgcctgcgca gcggaatgaa aaccgcgaac ggcaacgtgg tgcgcttttt tgaagtgatg
    18121 aagggcgaca acgtggcgat ggtgattaac ggcgatcaag gcacgatcag tcgcattgac
    18181 gtgctggata gcgatattcc tgctgacacc ggtgttaaaa tcggtacacc gtttagcgac
    18241 ctttacagca aagcatttgg caattgccaa aaagctgatg gtgatgataa tcgtgccgtc
    18301 gaatgtaaag ccgaaggcag tcaacatatt agctaccagt tcagcgggga atggagtggt
    18361 cctgaagggt taatgccttc ggacgatacc ctgaaaaact ggaaagtcag taaaattatc
    18421 tggcggcgtt aatttgcgtc tgaacaaacc gccgccgcga aaaaacacgt aaaatagcgc
    18481 tcaacaatgc cacggattgc gtggcattat tattttcagg aggaacaatg tctcaggttc
    18541 agagtggcat tttgccagaa cattgccgcg cggcgatttg gatcgaagcc aacgtgaaag
    18601 gggaagttga cgccctgcgt gcggccagta aaacatttgc cgacaaactg gcaacttttg
    18661 aagcgaaatt cccggacgcg catcttggtg cggtggttgc ctttggtaac aacacctggc
    18721 gcgctctgag cggcggcgtt ggggcagaag agctgaaaga ttttccgggc tacggtaaag
    18781 gccttgcgcc gaccacccag ttcgatgtgt tgatccacat tctttctctg cgtcacgacg
    18841 taaacttctc tgtcgcccag gcggcgatgg aagcctttgg tgactgcatt gaagtgaaag
    18901 aagagatcca cggcttccgt tgggttgaag agcgtgacct gagcggcttt gttgacggta
    18961 cggaaaaccc ggcgggtgaa gagacgcgtc gcgaagtggc ggttatcaaa gacggcgtgg
    19021 atgcgggcgg cagctatgtg tttgtccagc gttgggaaca caacctgaag cagctcaacc
    19081 ggatgagcgt tcacgatcag gagatgatga tcgggcgcac caaagaggcc aacgaagaga
    19141 tcgacggcga cgaacgtccg gaaacctctc acctcacccg cgttgatctg aaagaagatg
    19201 gcaaagggct gaagattgtt cgccagagcc tgccgtacgg cactgccagt ggcactcacg
    19261 gtctgtactt ctgcgcctac tgcgcgcgtc tgcataacat tgagcagcaa ctgctgagca
    19321 tgtttggcga taccgatggt aagcgtgatg cgatgttgcg tttcaccaaa ccggtaaccg
    19381 gcggctatta tttcgcaccg tcgctggaca agttgatggc gctgtaagcg tttctgattc
    19441 attgattaaa ggccaggcag taacattact gactggcctt tttattactt ttgctttaac
    19501 gccgcataca cctgatcaac tacccaacca tacgttgcaa tcggcagctg accgctttcg
    19561 aacatattgg gattcttttg cggatcggca accggcgaat gtggcttgtt gcttaacatc
    19621 acaatcgcca tatgattcac cggatcgata acggtcaccg ttccggtcca gccagtgtga
    19681 ccgtaggttt gcgggcttgc cagcgtgcca aacgtcggcg tcatggtggc attaccattc
    19741 acgcgccagc cgaggccaaa agtggcatct tccttagagc tggtggtgaa catcttcacc
    19801 gtttccgcac tgaacagctg cacatcgcca tagcccccgc cgttcagcat cgtttgcatt
    19861 aacaccgcaa tatcgccggt attggaaaac aaacctgcgt gcccggaaac gccgcccatc
    19921 gaataaaagg ctttttcatc gtgcacctga ccccagagag tggaggtgcg gatattcggg
    19981 aaatggatca cgccatcgcg ggtattgccg ttcaattccg tggcggcaat ttgctgcggt
    20041 ttaaagcctt tcagcagcgg gttaaatacc gtatgtgtca ggccgagcgg gcgataaatc
    20101 gactcttcaa catagcggtc aagcggctga ccggtgaccg actcgacgat aaatccaagc
    20161 agcatataat cgacatcact gtaaatatgc ttgctgccgg gctgatactc cagcggcgtg
    20221 cgcttgatca tctccagcgt ctggccttta tcctgggaat ataacgcgcc cgcgaccgct
    20281 ttattcggat attgtgggtc cgccgggaaa ccgccgctgt gatgcagcag gtcagaaatc
    20341 cgcagcgtgt ttttgccttt gatggtgtca tttggactat cggcaaaccc cggaatatat
    20401 ttcgcaatcc gatcgtcagg atgcagtttg ccttcgctca tcagcttttg cagggcgaag
    20461 ttcgtggcgt acattttggt gtttgaggcc aggtcataca gcgtcccggt ggtggctttg
    20521 acaggctgtt ccatcaacac gctgccatcg tactttttcg ccgctcccca ggctttgcga
    20581 tacacaatct gattatcttt aatgatcagc aggtttacgc cgggataacc ggcgtccact
    20641 tgctggctaa tccagcgatc catctggtta agccgttcga cgttgaaccc tgctttctct
    20701 ggcgaacttt ctgtcagaac agggtatttt gcggcgctga cgctacaact aacagccagc
    20761 agagaaagat agagcattgt ccgtttcatg atatcagtcc agattgacgt tacggcagcc
    20821 aaagagcgtg gtgaaaataa acccgcaaac ccatgccacc agaatcccgc cagcataaac
    20881 cgccattgcc ggaaggatgc cttgtgcgga agtcatcagc ggcagcgcca ccagaccaga
    20941 cggcccaaag gcgctgttta agcccatcgg cagaccccac caggctatca ggccgataaa
    21001 caaaccgccc gctgcgccgc ctaaacaggc ggtaataaac ggtttcatgc ggggcagggt
    21061 gacgccgtaa atcaacggtt caccaacgcc cagcagaccg ggaataatcg ccccgcgtac
    21121 ctgactgcgt aatgcactgt gcggttgcgc ccgccagtag agtgccagcg ccgcgcccac
    21181 ctggcccgcg cctgccattg aaaggatggg aaataagctg ttgaatccct ggctgtccat
    21241 taacgcgagg taaacaggaa taaagccctg atgcacgcca aataccacgg cgatcaggaa
    21301 cagcccggct aaaaccgcac aaccgaacgg attactgttc aggtgcataa acagccatga
    21361 cataccttcg aataaccagc cacccagcgg cataatgatc aggtacgcaa gcgtagcggt
    21421 gatcagcagg gtgattaacg aggtcagcag catgtcgaga tcgtccggca taaagcggcg
    21481 caccatgcct tcaatgcggg cgcaggccca cgcggcaatc agcacgccga taatattgcc
    21541 gcgcggatcg atgggcagac caaagaaatc gtgaaaaccg gcgtagtaac cggtggttgc
    21601 agcggggtta taaccgagca aaaagagtgc ggcaataatt gcgccattga cgcccgtgcc
    21661 gccaaatgcc tgggcggcgt tatagcccac cagaatcacc aggaaagtga acaaaccttt
    21721 gctgaacacc ttcataaaat tcagcgcgtc gggtagcgtg ccctgagcat ctgccggaac
    21781 gtgcatcacc gtggcaatta acgtcgctat ccccagcagc agaccggcgg caataaaacc
    21841 ggggatcagc ggcgtgaaga tggtggcgaa tttggcgaga aattgttgca cgccggaggt
    21901 ttgtttggct tttaactgac gtttgttctg ggcggcgatt tctgcggcat cctgtaccgg
    21961 tgcctcgccc agcagctcgc tcatcgcttt tgcggcacga tgcgctttac caggcccaaa
    22021 aacgacctgc acctggtcgc tggtcaaaat gacgcccttc acgccttcca gcgttttgat
    22081 attgggatca accagtgaac tgtcatgtac acccagacgc aggcgcgtca tacagttacc
    22141 acaactggcg atatttcccg gtccgccgac acgggtaaga atggtgttca gaagttcact
    22201 gctgatctct ttggccatgg gttattcctt gtctaaaacc tgacgaataa agccgccgtg
    22261 ttgatccagg cgttttttag cttctgcggc atcgagattt ttcagcacca tcacaatggc
    22321 cgttttacag ttgcgctcgc aggcaattaa cgccgcttcc gcttgttcgg cattacatcc
    22381 ggtggcgttt ttaacgatat tgacctggcg cacatgcagt ttttcgttgg tggcgaccac
    22441 atcgaccatc aggttgccga acactttgcc agatttaatc atcagcccgg tggaaagcat
    22501 attgagcacc agtttctgcg ctgtgcctgc tttcattcgt gaagaaccag tgaccacttc
    22561 ggcacctaca atcggtgtaa tggcaaactc ggcggtggtg gaaacggcac tccccggatt
    22621 acaggaaatt cccactgtgc ggcagccgag ctggcgtgcg tattccagtc cggcaatcac
    22681 atacggcgtg cgaccgctgg cagcaatgcc aaccaccaca tcctgtgccg ttaaattaat
    22741 atttttcaga tcgttaacac caccttcccg gctatcttca gcgccttcca ccgcgtgctg
    22801 aatggcatat tcgcctccag caatcaaacc aaccaccaga cccggtttca cgccgtaggt
    22861 gggcggacat tcgctggcat ccagaatccc cagacgaccg gatgttcccg caccgagata
    22921 aatcagacga ccgccgccgc tgacctgggc gtggataaca tcgatcgccg cggcgatatc
    22981 cggcaggacg cgctcaacgg caagcggtac ggttttatct tcatcgttga taatccggca
    23041 catttccagc gtcgatacgc ggtcaatttc agccgaggcg gtgttcgagc cttcagtaat
    23101 catcttttct agttgcatcg gtggtgacct tactatttac gatcaaataa ttgcagatgt
    23161 atggaatcat atatcccgaa ttgacagact taaaggaatc attgagtcta cactcgcaga
    23221 gtgtgaagag cgtcataaga acttaagcgg aatttatgct gccagcccag gagagtggaa
    23281 gatgttgtac ctgacgaaaa taagcaatgc ggggagtgaa ttcaccgaaa acgagcaaaa
    23341 aatcgctgac tttctgcagg ccaacgtgag tgagctgcaa tccgtctctt cgcgcaagat
    23401 ggcgaaacag ctcggcatca gccagtcgag tattgtgaag tttgcccaga agctcggcgc
    23461 acaaggattt actgaattgc gtatggcgtt aattggcgaa tacagtgcca gccgggagaa
    23521 aacgaatgcc acggcgctgc acctgcacag ttcgattacc agtgatgatt cgctggaagt
    23581 gattgcccgc aaactgaatc gcgaaaaaga gctggcgctg gagcaaacct gcgcgttgtt
    23641 cgactacgcg cggctgcaaa aaatcattga tgtcatcagt aaagcgcaat ttattcagat
    23701 aacgggcctg ggcggatcgg cgctggtggg gcgcgatctg tcgttcaaac tgatgaaaat
    23761 tggatatcgc gtggcctgcg aagccgacac gcacgtacaa gccaccgttt cccaggcgtt
    23821 gaaaaaaggc gatgtacaaa tcgctatctc ttacagcggc agtaaaaaag agattgtctt
    23881 gtgcgcagaa gcggcacgaa agcagggggc aacggtgatt gccattactt ctctggctga
    23941 ctctccgctg cggcggctgg cgcatttcac gctcgatacc gtttccggtg aaaccgaatg
    24001 gcgtagctcg tcgatgtcta cccgcacggc gcaaaattct gttaccgatt tactgtttgt
    24061 tggtcttgtg caactcaatg atgtcgagtc attgaaaatg attcagcgca gcagtgagct
    24121 aacacaacgc ctgaaatgat gcataaagca gcgactggat tgagattttc ctgaattagt
    24181 gagctgatcc gcagcaatat tttgtttatc ctgtattttc agagggaatg gagtgtaacg
    24241 ctctgtatta acaaggagag cattaaaatg ggtaaactca cgggcaagac agcactgatt
    24301 acgggcgcat tgcagggaat tggcgaagga attgccagaa cttttgcgcg tcatggcgca
    24361 aacctaatct tgctggatat ctcccctgag atcgaaaagc tggcggacga actgtgtggt
    24421 cgtggtcatc gctgtacggc ggttgtcgcc gatgtgcgtg acccggcgtc ggtagccgca
    24481 gctatcaaac gcgcgaagga aaaagaaggg cgcattgata tcctggtgaa taacgcaggc
    24541 gtttgtcgtc tgggcagttt cctcgatatg agcgatgaag atcgcgattt ccatattgac
    24601 atcaatatta aaggcgtatg gaacgtcacg aaggcggtgc tgccagagat gattgcgcgc
    24661 aaagatggtc gcattgtgat gatgtcttca gtcactggtg atatggtggc cgatccgggt
    24721 gaaacggcgt acgccttaac gaaagcggcg attgttggcc tgacaaaatc gctggcggtg
    24781 gagtacgcgc aatccggtat tcgcgttaac gccatctgcc cggggtacgt ccgcacgcca
    24841 atggcggaaa gcattgcccg ccagtcgaac ccggaagatc cagagtcggt gctgactgat
    24901 atggcaaaag caatcccgct gcgtcgcctc gccgatccgc tggaagtcgg cgaactggcg
    24961 gccttcctcg catcggatga atccagctat ttaaccggta cacagaatgt gattgatggc
    25021 ggcagcacac tgccggagac ggttagcgtc ggtatctgat tcacctctgt ttcctccctg
    25081 catgtgtggg gaggatttcg ttttaaaccc ggttcttatt cccttttcaa cttccaaatc
    25141 accaaacggt atataaaacc gttactcctt tcacgtccgt tataaatatg atggctatta
    25201 gaaagtcatt aaatatataa gggtgcgcaa tggccgttaa cttactgaaa aagaactcac
    25261 tcgcgctggt cgcttctctg ctgctggcag gccatgtaca ggcaacggaa ctgctgaaca
    25321 gttcttatga cgtctcccgc gagctgtttg ccgccctgaa cccgccgttt gaacagcaat
    25381 gggccaaaga taacggcggc gacaaactga cgattaaaca atctcatgcc gggtcatcaa
    25441 aacaggcgct ggcgatttta cagggcttaa aagccgacgt tgtcacttat aaccaggtga
    25501 ctgacgtaca aatcctgcat gacaaaggca agctgatccc ggcggactgg cagtcgcgcc
    25561 tgccgaataa tagctcgccg ttctactcca ccatgggctt cctggtgcgt aagggcaacc
    25621 cgaagaatat ccacgactgg aacgacctgg tgcgctccga cgtgaagctg attttcccga
    25681 acccgaaaac gtcgggtaac gcgcgttata cctatctggc ggcatggggc gcagcggata
    25741 aagctgacgg tggcgataaa gccaaaaccg aacagtttat gacccagttc ctgaaaaatg
    25801 ttgaagtgtt cgataccggc ggtcgtggcg caaccaccac ttttgccgag cgtggcctgg
    25861 gcgatgtgct gattagcttt gaatcggaag tgaacaacat ccgtaaacag tatgaagcac
    25921 agggctttga agtggtgatc ccgaaaacca atattctggc ggaattcccg gtggcgtggg
    25981 tcgataaaaa cgtgcaggcc aacggtacgg aaaaagcagc aaaagcctac ctgaactggc
    26041 tctacagccc gcaggcgcag accatcatca ccgactatta ctaccgcgta aataacccgg
    26101 aagtcatgga caaactgaaa gataaattcc cgcagaccga gctgttccgc gtggaagaca
    26161 aatttggctc ctggccggaa gtgatgaaaa cccacttcac cagcggcggc gagttagaca
    26221 agctgttagc ggcggggcgt aactgatgtt tgcagtttca tcaagacgcg tgctgccggg
    26281 ctttacctta agcctcggca ccagtctgct gtttgtgtgc ctgattttgc tgctgccgct
    26341 ctccgcgctg gtgatgcagc tggctcagat gagctgggcg cagtactggg aggtgatcac
    26401 caacccacaa gtggtcgcgg cctacaaagt gacgctgctg tcggcgtttg tggcatcgat
    26461 ttttaacggc gttttcggtc tgctgatggc gtggatttta acacgttatc gctttccggg
    26521 ccgcacgctg cttgatgcgc tgatggattt accctttgcg ctgccaacgg cagtcgccgg
    26581 tttaacgctg gcctcgctct tttccgtaaa cggtttttac ggtgaatggc tggcgaagtt
    26641 tgatatcaaa gtcacctata cctggcttgg tattgcggtg gcgatggcct ttaccagcat
    26701 tccgtttgtg gtgcgtaccg tgcagccggt gctggaagag ttaggcccgg aatatgaaga
    26761 agcggcggaa acgcttggtg caacgcgctg gcagagtttc tgcaaagtgg tgctgccgga
    26821 actttctcca gcgctggtgg cgggcgtggc gttgtcgttt acccgtagtc ttggtgagtt
    26881 tggcgcggtg atatttatcg ccgggaacat cgcctggaag acggaagtga cgtcgctgat
    26941 gatttttgtg cgcttgcagg agtttgatta cccggcggcg agcgcgattg cttcggtgat
    27001 cctcgcggca tccctgctgc tgctgttctc aattaacact ctgcaaagtc gctttggtcg
    27061 gcgtgtggta ggtcattaat ggcggaagtt acccaattga agcgttatga cgcgcgcccg
    27121 attaactggg gcaaatggtt tctaattggc atcgggatgc tggtttcagc gttcatcctg
    27181 ctggtgccga tgatttacat cttcgtgcag gcattcagca aggggctgat gccggtttta
    27241 cagaatctgg ccgatccgga catgctgcac gccatctggc tgacggtgat gatcgcgctg
    27301 attgccgtac cggtaaacct ggtgttcggc attctgctgg cctggctggt gacgcgcttt
    27361 aacttccctg gacgccagtt actgctgacg ctactggaca ttccgtttgc cgtatcgccg
    27421 gtggttgccg gtctggtgta tctgctgttc tacggctcta acggcccgct gggcggctgg
    27481 ctggatgaac ataatctgca aattatgttc tcctggcccg gaatggtgct ggtcaccatc
    27541 tttgtgacgt gtccgtttgt ggtgcgcgag ctggtgccgg tgatgttaag ccagggcagc
    27601 caggaagacg aagcggcgat tttgcttggc gcgtctggct ggcagatgtt tcgccgcgtc
    27661 acattgccga acattcgctg ggcgctgctt tatggcgtgg tgttgaccaa cgcccgcgca
    27721 attggcgagt ttggcgcggt gtcggtggtt tccggctcga ttcgcggcga aaccctgtcg
    27781 ctgccgttac agattgaatt gctggagcag gactacaaca ccgtcggctc ctttaccgct
    27841 gcggcactgt taacgctgat ggcgattatc accctgtttt taaaaagtat gttgcagtgg
    27901 cgcctggaga atcaggataa acgcgcacag caggaggaac atcatgagca ttgagattgc
    27961 caatattaag aagtcgtttg gtcgcaccca ggtgctgaac gatatctcac tggatattcc
    28021 ttcaggtcag atggtcgcgt tgctggggcc gtccggttcc gggaaaacca cgctgctgcg
    28081 cattatcgcc gggctggagc atcaaaccag cgggcatatt cgcttccacg gcaccgacgt
    28141 cagccgcctg cacgcgcgcg atcgcaaggt cggtttcgtg ttccagcatt acgcgctgtt
    28201 ccgtcatatg acggtgttcg acaatatcgc ttttggcctg acggtgctgc cacgtcgcga
    28261 gcgcccgaat gccgcagcca tcaaagcgaa agtgacaaaa ttgctggaga tggtgcagct
    28321 tgctcatctg gcagatcgtt atccggcaca gctttccggc ggccagaaac agcgcgtggc
    28381 gctggcgcgc gcgctggctg tagaaccgca aattctgctg cttgatgaac cgtttggcgc
    28441 gctggatgcg caggtgcgta aagagctgcg tcgctggctg cgtcaactgc atgaagagct
    28501 gaaattcacc agcgtgttcg tgacccacga ccaggaagag gcgaccgaag tagccgatcg
    28561 tgtagtggtg atgagtcagg ggaatattga acaggctgat gcgccgaatc aggtatggcg
    28621 cgaaccagcg acccgttttg tactcgaatt tatgggcgaa gtgaaccgcc tgcagggaac
    28681 cattcgcggc gggcagttcc atgttggcgc gcatcgctgg ccgctgggct acacacctgc
    28741 gtatcagggt ccggtggatc tcttcctgcg cccgtgggaa gtggatatca gccgccgtac
    28801 cagtctcgat tcgccgctgc cggtacaggt actggaagcc agcccgaaag gtcactacac
    28861 ccaattagtg gtgcagccgc tggggtggta caacgaaccg ctgacggtcg tgatgcatgg
    28921 cgacgatgcc ccgcagcgtg gcgagcgttt attcgttggt ctgcaacatg cgcggctgta
    28981 taacggcgac gagcgtatcg aaacccgaga tgaggaactt gctctcgcac aaagcgcctg
    29041 ataggttgag tgaatgttaa acgcccggag gcgcttcccg cgatccgggc tttttaatgg
    29101 caaggtttgt aacctgtaga cctgataaga cgcgcaagcg tcgcatcagg caacaccacg
    29161 tatggataga gatcgtgagt acattagaac aaacaatagg caatacgcct ctggtgaagt
    29221 tgcagcgaat ggggccggat aacggcagtg aagtgtggtt aaaactggaa ggcaataacc
    29281 cggcaggttc ggtgaaagat cgtgcggcac tttcgatgat cgtcgaggcg gaaaagcgcg
    29341 gggaaattaa accgggtgat gtcttaatcg aagccaccag tggtaacacc ggcattgcgc
    29401 tggcaatgat tgccgcgctg aaaggctatc gcatgaaatt gctgatgccc gacaacatga
    29461 gccaggaacg ccgtgcggcg atgcgtgctt atggtgcgga actgattctt gtcaccaaag
    29521 agcagggcat ggaaggtgcg cgcgatctgg cgctggagat ggcgaatcgt ggcgaaggaa
    29581 agctgctcga tcagtttaat aatcccgata acccttacgc ccattacacc actaccgggc
    29641 cggaaatctg gcagcaaacc ggcgggcgca ttacccattt tgtctccagc atgggaacaa
    29701 ccggcactat caccggcgtt tcacgcttta tgcgcgaaca atccaaacca gtaacgattg
    29761 ttggcctgca gccggaagaa ggcagcagta ttcccggcat tcgccgctgg cctgcggaat
    29821 atctgccggg gattttcaac gcttctctgg tggatgaggt gctggatatt catcagcgcg
    29881 atgcagaaaa caccatgcgc gaactggcag tgcgggaagg aatattctgt ggcgtcagct
    29941 ccggcggcgc ggttgccgga gcactgcggg tggcaaaagc taaccctggt gcggtggtgg
    30001 tagcgatcat ctgcgatcgt ggcgatcgct acctttccac cggggtgttt ggggaagagc
    30061 attttagcca gggggcgggg atttaagatt attgcgcagg caccgtttta tccgtctgcg
    30121 caattagcgc atgtaaaaaa ggctgtgcgt tctcatcgct cttaccgggg atcttcacaa
    30181 tatacggctt cccggtaatc gatgacgacg aagcaacctt atcaataaac tgctcggcag
    30241 tgtcaatgcg gttacgggta ttgcccagct tcagacgcag atgagaaacc gcttcatcgc
    30301 aggtatgttc atcaccgttg cgcacaaata tcaaatcctt tttctgtgct aatccctcca
    30361 gcatggcgtt gatgcgggct tcttcatggg cagttaactt cgcgtaagcg gggagtgtca
    30421 ttagtagtgt aatgaccaga caaatgattt tcttcattga attaccttat ccttgttttg
    30481 tgatagccga tttgactgcc agaatcaggt aaagtttcgg ttggaatacg atgaggatca
    30541 tcggcaagga gaaaaaatta aatgagtagt ttgttgttgt ttaacgataa gagcagggcg
    30601 ctgcaggcgg atatcgtcgc cgtgcagtcg caggtggttt acggcagcgt gggcaacagc
    30661 attgccgtgc ctgctatcaa acagaacggt ctgaatgttt ttgccgtgcc gacggtattg
    30721 ctgagcaata cgccgcatta tgacactttc tacggtggtg cgattccgga tgaatggttt
    30781 agcggttatt tgcgtgcgct tcaggagcgt gatgcgctgc gccagctgcg cgctgtaacc
    30841 acgggctata tgggaacggc atcgcaaatc aaaatccttg ccgagtggct gactgcgcta
    30901 cgcaaagacc atcctgacct attgatcatg gtcgatccgg tgattggcga tattgatagc
    30961 ggaatttatg tcaaacctga ccttcccgag gcgtatcgac aatatttact gccgctggcg
    31021 cagggaatta cccccaatat ctttgagctg gaaatcctga ccggtaaaaa ttgccgcgat
    31081 ctcgacagtg ccattgctgc cgcaaaaagt ctgctttcag acacattaaa atgggtggtg
    31141 attaccagcg cctccggtaa tgaagaaaat caggagatgc aggtggtggt ggtcagtgcc
    31201 gacagcgtga atgtcatttc ccattcacgg gtaaaaaccg acctgaaagg gactggcgac
    31261 ctgttttgtg ctcagcttat cagtggtttg ctgaaaggga aggcgttaaa cgatgcggtg
    31321 caccgtgcgg ggttgcgcgt actggaagtg atgcgctaca cccagcagca tgagagcgat
    31381 gaattgattt tgccgccgct ggcggaagca taaaaaatgg cgccgatggg cgccattttt
    31441 cactgcggca agaattactt cttgatgcgg ataaccgggg tttcacccac ggttacgcta
    31501 ccggacagtt tgatcagttc tttgatttcg tccatgttgg agataacaac cggagtcagg
    31561 gtagacttgg ctttctcttc cagtagcggc agatcaaatt caatgacagt atcgccaact
    31621 ttcacgcgct gaccttcttc agcaatacgc ttgaagcctt cgcctttcag ttcaacggtg
    31681 tcgataccga agtggacgaa cagttcaacg ccgctatcag attcgataga gaacgcgtga
    31741 ttggtttcaa agattttacc aatggtaccg tctacaggcg cgaccatttt gttacccgtt
    31801 ggtttgatag caataccatc accaacgatt ttttccgcaa aaacgacatc cggcacgtct
    31861 tcgatattga cgatctcgcc agagagcgga gcaatgatct caatagttcc ggtatccttc
    31921 ttgtcgtcgg aaaccagaga tttcagttta tcgaacaaac ccatgatctt ctcctaagca
    31981 gtaaattggg ccgcatctcg tggattagca gattgttttt tcttcaatga acttgttaac
    32041 cagcgtcatt aactcgtccg ttgtcggttg agcaagagcc tgctctgcta acaccttcgc
    32101 atcttcgaag ttcgtgttac ggataatctt cttaatgcgc gggatagaaa tggcgctcat
    32161 agagaattcg tccagaccca tccccagcaa cagaagtgta gcacgttcat cgccagcaag
    32221 ctcaccacac atgccagtcc atttgccttc agcatgagaa gcatcaataa cttgcttgat
    32281 caagttcagc acggacggtg acattggttg gtaaaggtgt gaaatcatat cattaccacg
    32341 gtcaactgcc agagtgtact gcgttaaatc attggtgccg atactaaaga aatcaacttc
    32401 tttggctaaa tgacgtgcaa ttgttgcggc agccggtgtt tccaccatta cgccgatttc
    32461 aattgactcg tcaaacgctt taccttcgtc gcgcagttcc tgtttgtaga tttcgatctc
    32521 tttgcgcagt gcacgcactt cttcaacaga gatgatcatc gggaacataa tgcgcaattt
    32581 accgaaagcc gaggcacgca ggatagcgcg gagctgatcg cgcaggatct ctttacgatc
    32641 catcgcgata cggatagcgc gccagccgag gaacgggttc tcttctttcg ggaagttcat
    32701 gtatggcagc tctttgtcgc cgccgatgtc catggtacga acgataaccg cctgcgagcc
    32761 acacgcttca gccactgctt tgtaagcagc aaactgttct tcttcagtgg gcagcgcgtc
    32821 gcggtccatg aacaggaact cagtacgata cagaccaacg ccttcagcgc cgttacgctc
    32881 tgcaccttca acgtcacgaa ccgtaccaat gttagcgcac acttctacct ggtgaccgtc
    32941 cagcgtaata gctggcagat ctttcagttt agcaagctct gctttttcag aagccacttg
    33001 ctcctgaaca gcgcgcattt tatcaataac ttcgttggtt ggattgacgt aaacctgatt
    33061 atttacggca tccagaatca gatagtcgtc atttttcacc tgagaggtga cgctaccggt
    33121 acccacgata gcaggcagtt ccagagaacg cgccatgata gaggtgtggg aagtacggcc
    33181 acccgcgtcg gtgatgaaac ccagcacctt cttcaggttc agctgtgcgg tttcggacgg
    33241 cgtcaggtca gcggcaacca gaatgacttc atcctgaatg gcgctcaggt cgataatctt
    33301 caggcccagg atgttgcgca gcaggcgctt accgatatca cgtacgtcag ccgcacgttc
    33361 tttcaggtat tcatcatcca gctcttccag ggcagaagcc tgaccttcga taacttcatg
    33421 agcagctgcg tcagctgtca tgtgcttatc tttaatcagg gctatgattt cctgctccag
    33481 ctcctcatct tcgagcagca taatatgccc ttcaaagatg gcttcttttt cttcaccgaa
    33541 cgtttcacca gctttcgttt tgatcgtttc cagctgggct gatgccttgg cacgaccgct
    33601 cagaaaacgt tcaacttcct gatcaacctg gtcggcagaa atttttttcc ggtcaatgac
    33661 aatttcgtct tctttcagaa gcagagcttt accgaaagcg atacccgggg atgctaaaat
    33721 gcctgaaatc ataaccctac cttacttgtg actgattttt aaaagaaccc gggaaattac
    33781 tcgagttccg ccatcagttt aaccagatgt tcaaccgctt tctgctcgtc ttcgccttct
    33841 gcggagatag tcacaacggt accttgagtc aggcccagag tctgcagttt aaacaggctt
    33901 ttcgcgctgg cgcttttgcc gttggaagtc acagtaattt cagaagtgaa gcccttagct
    33961 tcttttacaa actgggcagc agggcgggtg tgcagaccgt tcggagcggt aatggtaact
    34021 tcttgctgga acattgtatt tccccaactt ataggtttag tgttgtggaa ctaaagtcta
    34081 gcctggcggt tcgactttag cctgtattgt tagcgccagt ttttaacaga cgcgacgcac
    34141 gaagagcgca atacagtcca ttgctggcgg gtgttgaccg ctgacgtttc gtacgtcatt
    34201 aaacattatg ccgctaaagg aagaattgaa ccaaatcata aaatcgattc agctttggct
    34261 tttcctgtaa cgattaattt cgcgcatcaa aataattagt ctggttaaat accagttccg
    34321 acaggctgaa tcaatgccag gaggggcgaa agtttgaagc aggccacaaa aaagcaccta
    34381 aaaaggtgct tttttacgca tttttaacaa gctggcatta ctgttgcagt tctttctcag
    34441 tgaagagatc ggcaaacaat gcggtgctta aataacgctc acccgatgac ggtagaataa
    34501 ccacaatatt cttgttggta aagctttcat cttcttgtag tttcaacgcc gcagcaacag
    34561 ctgctccaga agagatacct gcaagaatac cttcttcttc catcagacga cgcgcggtag
    34621 aaatcgcttc ttcattggtg atgccaatga ctttatcgac cagcttgaga tcgaggttag
    34681 ctgggataaa accagcgcca ataccctgaa ttttatgcgg gccaggttta atctcttcac
    34741 cagccagtgc ctgggcgata actggagaat cggttggctc aacggcgaca gagataagat
    34801 cggtcttgcc tttggtgcct ttaatgtagc ggctgacgcc agtcagcgta ccgccagtcc
    34861 caacgccagc aataaataca tcaacctgac cgtcggtatc ttcccagatc tccggaccgg
    34921 tggttttttc gtgaatttca gggtttgccg gattgctgaa ttgttgcagc agcaggtatt
    34981 tctctggatt gctggcgaca atttcttctg ctttttggat tgcgcctttc atgcctttag
    35041 caccttccgt cagcaccagg tttgcaccta acgctttcag cagcttgcgg cgttcaatac
    35101 tcatggtttc tggcatggtc agggtgagtt tgtaaccgcg agcggcagct acataggcca
    35161 gtgcaatccc ggtattaccg ctggtcggtt caaccagttc aacgcctggt ttcagcacgc
    35221 cgcgcttttc ggcatcccaa atcatgttgg caccgatacg gcacttaacg ctgaagctgg
    35281 ggttacgaga ttccaccttc gctagaatgc gtccgttacc gatgcgattc aggcgaacca
    35341 gcggcgtgtg accgatagtc agcgagttat cttcaaaaat cttactcatg gcctgtcctt
    35401 aactgtatga aattgggata caacaggtag catacccgct cagagaatat gcggaagtaa
    35461 ggatttagca tatctatata cagaagggaa ataatgacat gcaagatgga ataaggggcg
    35521 gcataagcca cccctgtttc acacaaacgg tttacaaaat aaccggtaga ttgttaccgc
    35581 cacatcgcgt gtttatcgcg atagcaatcg acccacatcg ccgtcgcgcc acaaacggca
    35641 acgggcatga tgaacagatt aagcagcggg atcatcgtaa acaggctggt taaagcacca
    35701 aactgcatat tggtgatttt gcgtgtgcgc agggcggtgc gcatctcttt aaacggcact
    35761 ttgtggttat cgaaggggta atcgcaatac tggatggcta acatccaggc gctaaacagg
    35821 aaccacagta ccggcgcgac ggtttgccca ataccaggga ttaagtaaag aattagcaat
    35881 acaattgcgc gcggcagata ccaggcaaat ttttgccatt cgcgtttcat gattcgcggc
    35941 acatctttca tgataccgaa aatcccggta tctggcggtg tagcgccagt caatcgtgct
    36001 tccagttgtt cagccaataa accgttaaac ggagcggcaa tccagttagc aatcgtggag
    36061 aagaaatagc caaacactaa cagcacagag atgacagcca gaggccacaa cagataactc
    36121 agccattgta gccagtccgg aacgtaactc atgagagtcg ggatccagac atcgagctgt
    36181 gtaaagagcc accagaatgc gccccccatc aacaaaatat tgaccagcag cggtaaaata
    36241 acgaaacgcc gaatcccagg ttgcgagacg agcttccagc cttgcgcaaa atagtaaaaa
    36301 ccgctgcgtg gggcagatgt gaatgatgaa accataatca ggatgagctc cttttgacca
    36361 atcccaggaa aattctgcgt attttaccgg gtaattgcgc aatggacagt taggatatgt
    36421 tcgaaaaaac agcaaaaagc acgatttcat ctatctttgt gctgtgaaag ttaatagtgc
    36481 acttgcactt gaggtaatcg gcaaatactc ttagtgagta aatgtttgcc gtggtggcaa
    36541 ggtgttagaa caacagagaa tataatgatg caggatttgc gtctgatatt aatcattgtt
    36601 ggcgcgatcg ccataatcgc tttactggta catggtttct ggaccagccg taaagaacga
    36661 tcttctatgt tccgcgatcg gccattaaaa cgaatgaagt caaaacgtga cgacgattct
    36721 tatgacgagg atgtcgaaga tgatgagggc gttggtgagg ttcgtgttca ccgcgtgaat
    36781 catgccccgg ctaacgctca ggagcatgag gctgctcgtc cgtcgccgca acaccagtac
    36841 caaccgcctt atgcgtctgc gcagccgcgt caaccggtcc agcagccgcc tgaagcgcag
    36901 gtaccgccgc aacatgctcc gcgtccagcg cagccggtgc agcagcctgc ctatcagccg
    36961 cagcctgaac agccgttgca gcagccagtt tcgccacagg tcgcgccagc gccgcagcct
    37021 gtgcattcag caccgcaacc ggcacaacag gctttccagc ctgcagaacc cgtagcggca
    37081 ccacagcctg agcctgtagc ggaaccggct ccagttatgg ataaaccgaa gcgcaaagaa
    37141 gcggtgatta tcatgaacgt cgcggcgcat cacggtagcg agctaaacgg tgaactgctt
    37201 cttaacagca ttcaacaagc gggcttcatt tttggcgata tgaatattta ccatcgtcat
    37261 cttagcccgg atggcagcgg cccggcgtta ttcagcctgg cgaatatggt gaaaccggga
    37321 acctttgatc ctgaaatgaa ggatttcact actccgggtg tcaccatctt tatgcaggta
    37381 ccgtcttacg gtgacgagct gcagaacttc aagctgatgc tgcaatctgc gcagcatatt
    37441 gccgatgaag tgggcggtgt cgtgcttgac gatcagcgcc gtatgatgac tccgcagaaa
    37501 ttgcgcgagt accaggacat catccgcgaa gtcaaagacg ccaacgcctg atacacttaa
    37561 ggcaaattaa ctcctcttcg aacccccgct tgtcgggggt ttttagcatt gatggtgcga
    37621 tatggaatca atcgaacaac aactgacaga actgcgaacg acgcttcgcc atcatgaata
    37681 tctttatcat gtgatggatg cgccggaaat tcccgacgct gaatacgaca ggctgatgcg
    37741 cgaactgcgc gagctggaaa ccaaacatcc agaactgatt acgcctgatt cgcctaccca
    37801 acgtgtaggc gctgcgccgc tggcggcttt cagccagata cgccatgaag taccaatgct
    37861 gtcactggat aacgtttttg atgaagaaag ctttcttgct ttcaacaaac gtgtgcagga
    37921 ccgtctgaaa aacaacgaga aagtcacctg gtgctgtgag ctgaagctgg atggccttgc
    37981 cgtcagtatt ctgtatgaaa atggcgtttt agtcagtgcc gcgacccgtg gcgatggcac
    38041 caccggggaa gatatcacgt ctaatgtgcg tactattcgc gctattccgc tgaagctgca
    38101 cggagagaat atcccggcgc gtctggaagt gcgtggtgaa gtgttcctgc cgcaggcggg
    38161 gttcgaaaag attaacgaag atgcgcgacg cacgggcggg aaagtttttg ctaacccacg
    38221 taatgcggca gctggttcac tgcgtcagct tgatccgcgt attacagcga agcgaccgct
    38281 cacttttttc tgctatggcg ttggtgttct ggaaggtggc gagctgccgg atactcatct
    38341 tggccgttta ctgcaattta aaaagtgggg gttgccggtc agcgatcggg taacgctttg
    38401 tgaatcggcg gaagaagtgc tggcgttcta tcacaaagtg gaagaagacc gcccgacgct
    38461 gggctttgat atcgacggcg tggtgattaa ggtcaactca ctggaacagc aggagcagct
    38521 tggctttgtc gcgcgtgccc cgcgctgggc ggtagcgttt aaattcccgg cgcaggagca
    38581 gatgaccttt gtgcgtgacg tcgagtttca ggttgggcgt actggcgcga ttacgcctgt
    38641 tgcgcgtctg gaacctgtcc atgttgctgg cgtgctggtg agtaacgcaa ccttacacaa
    38701 tgcggatgaa atcgaacgtc ttggtttacg cattggcgat aaagtggtga ttcgccgcgc
    38761 tggcgacgtg atcccgcagg tggttaacgt cgtgctttct gaacgcccgg aagatacccg
    38821 tgaggttgta ttcccgacgc attgtccggt atgtggttct gacgttgagc gtgtggaagg
    38881 tgaagcggtt gcccgctgta ccggtggcct gatttgcggt gcgcagcgta aagagtcgct
    38941 gaaacacttt gtttcccgcc gtgcgatgga tgttgacgga atgggcgaca aaatcatcga
    39001 tcagctggtt gaaaaagaat atgtccatac tccggcagat ctgttcaaac tcaccgcagg
    39061 caaactgacc ggactggagc gtatggggcc aaaatcggca caaaacgtgg ttaacgcgct
    39121 ggaaaaagcg aaagaaacca cctttgctcg cttcctctat gcacttggca tccgtgaagt
    39181 cggcgaggcc accgcagcag gtctggcggc atatttcggc acgctggaag cgctggaagc
    39241 cgcttcgatt gaagagctgc aaaaggtgcc tgatgttggc attgtcgttg catcccacgt
    39301 tcacaacttc tttgccgaag aaagcaaccg caatgtcatc agcgagctgt tggcggaagg
    39361 tgttcactgg cctgcgccga tcgttatcaa cgcggaagag attgacagcc cgtttgctgg
    39421 taaaaccgtg gtgcttacgg gcagcttaag ccagatgtcg cgtgatgacg ctaaagctcg
    39481 actggttgaa ctgggcgcga aagtcgcggg cagcgtgtcg aagaaaaccg atctggtgat
    39541 tgccggagaa gcggcaggtt ctaaactggc gaaagcgcag gaactgggca ttgaagtcat
    39601 cgacgaagcg gaaatgctgc gtttgctggg tagctgagat ggaaaaagag cagctgattg
    39661 aaatagccaa tacgataatg ccgtttggca aatataaagg gcgtcgctta atcgacctgc
    39721 cggaggagta tctgctgtgg tttgcccgca aagatgaatt cccggcaggg aagctcggtg
    39781 agctaatgca aatcacgctg ctgattaaaa ccgaggggct gacgcaactg gtccagcccc
    39841 tgaagcgtcc gctttaagct ttatcggcgc tgctttcctg ctgtgcctgt aactgttcgg
    39901 tctggcgttt gtatcgacgc gccagcaccg cacagaccat caattggatc tgatggaaaa
    39961 tcattagggg cagcaccatc ataccgatca ccgatgtggg gaacagaatg tttgccatcg
    40021 ggatgccatt tgccagactc tttttcgaac cacaaaagac gatagtaatt tcatctgcct
    40081 tattgaagcc cagtcggcgt gccataaaga cgttaactac aatcacgata gccagaagaa
    40141 cgcagctgac caccacgata aacagcaatg atccccagcc aactttatgc cagataccat
    40201 taacgacggc ttcgctgaat gctgtataaa ccaccaacag aatggacgtc tggtcagttt
    40261 tcgcaatcca ttttttattg cgcgacaccc agtcaccaat ccacggccgg gaaagatgcc
    40321 ccaacacaaa cggcagcagc agttgcagca taattttacc gacctgctca aggctgcccc
    40381 ctgcaccgtg aacattcatc accagaccaa ccagcaatgg tgaaaggaaa atccccagca
    40441 ggctggatgc tgacgcagaa caaaccgccg ccgcgacgtt accgcccgcc attgacgtga
    40501 aggcgattgc agactgcacg gtagccggga gaatgcacaa gtagagaaaa ccggagtaga
    40561 gcatcgggtc gacatttacc ggtttccacc aggcaaacag tacacccaga atcggaaaca
    40621 gcacgaaggt gctgcaaatt acccacaaat gcagtcgcca gtgaccaccg ccagcaataa
    40681 tcgcctcacg cgacaacttc gcgccgtgca taaagaacag cagggcaatc gctgcggtgg
    40741 tcagattttc aaagaagggg acgaaatcgc ctctggccgg aaagaaagag gccagcaaca
    40801 ccaccgtgat cagggttaag gtgaaaggat cgaggatacg aaaaagtttc ataaagactc
    40861 cagatgatcg atggttctat tgtgcttctt ccagtttgag aaataaaatt gatttattgc
    40921 atccattcat gaagaaaaca gatgaattat tctttaaaac aattaaaggt tttcgtcaca
    40981 gtagcgcagg agaaaagttt tagtcgtgca ggagagcgta tcggcctgag ccagtcggca
    41041 gtgagtcaca gtgtgaagga actggaaaat catactggtg ttcgcctgct ggacagaacc
    41101 acgcgtgaag tggtgcttac agatgcaggg cagcagctgg ctttgcgtct tgagcgactg
    41161 ctggatgaac tgaacagcac gttgcgcgat accgggcgta tggggcaaca actgagcgga
    41221 aaagttcggg tcgctgccag ccagaccatt tccgcgcatc ttattccgca atgcattgct
    41281 gaaagccatc gccgctatcc agatattcag tttgtcctgc acgatcgtcc gcagcagtgg
    41341 gtgatggaaa gtattcgcca gggagatgtc gactttggca tcgtcatcga tccaggccca
    41401 gtgggcgatc tgcaatgtga agcgattctt tccgagcctt tctttctgct ttgccatcgc
    41461 gatagcgctt tggccgtcga agattatgta ccctggcagg cattacaggg agcgaaattg
    41521 gtgttgcagg attacgcgtc aggcagccga ccgctgattg acgcagcgct ggcgcgcaat
    41581 ggtattcagg cgaatattgt gcaggagatt ggacatccgg cgacgctgtt cccgatggta
    41641 gccgcaggca tcggcatcag tattcttccc gcactggcgc taccattgcc tgaaggtagc
    41701 ccactggtgg taaagcgcat tacgccggtt gttgaacgcc agttgatgct ggtgcgcagg
    41761 aagaaccgtt cactttccac cgccgctgaa gcgttatggg acgtggtacg agaccagggc
    41821 aatgcgctaa tggctggtcg ggaaggggat ccgttgtatc agatatagag tgaattcata
    41881 ctgccgggag ccccggcagt aatcaatcag aagttaaatt tgatggagta gattatcgca
    41941 tcctggtaaa aatcttcgcc cagtttgtca tccgcatatc gatactgaat ccccgtagta
    42001 atgtgtgatg ttgcattcca ccacagtgca actgcgccat tcagcccttc tttaccgcca
    42061 ttacccgctg cataggtagc gtcacggtca aactcgtact cgttccagtt ggtaagagtg
    42121 aatttctcgc tgcccagcat aaagttgtaa cctgcaaccc agccggcgac atagccgttg
    42181 tcgccagtgt aataggtttg atctgtataa cgcttagcaa agaacggttt gaaccaccaa
    42241 ccgctgccgg tgaaattgta gccgataccg tacaggaaca tatcgtcgtg gaaattcacg
    42301 cgattcgcag aaccgtaggt gccatacgca tgcaagtaga gattaaatcc ggtgtcaccc
    42361 aggtaaatac ggttggtatt tttaaaggta taacgctgct cgctacctgg tttattatgg
    42421 cgaccgttat agaagttttc ccagtcgaag aacccgtaca tttctcccca actaaagtta
    42481 gcgccacctt caaattcaag ataaccaaaa tcatctttgt gtgatttggt cgacgatttt
    42541 tcggtagtcc ggctggtcca gtccagataa tgcacgccga tatctgcaaa accgccttta
    42601 aattctgcat gagatacaac aggtatcgct aatatagagg aaagtgtcag agttaaaaga
    42661 tgttttttca tagcataatt ccctatgccg atcgttatta gtagattgct taataaagtg
    42721 gtttgctaaa aacgacatat tgttaatgca aaggaattaa tatcgccaat acagttttta
    42781 ccgtggatta atagagaaaa gatataaatc catcgatatc gaatcgctat tgaatgcgtg
    42841 agaaaagtgt attggtaaga gccgggacaa cctcgctata aaaaggcgtt agattccacc
    42901 ctacagaaaa aggatatgta tgtctcaggt tcaattttct cataacccgc tgttttgcat
    42961 agatattatc aagacttata aacctgattt cacgccacga gtggccttta ttttaggttc
    43021 cgggctcggc gcgctggccg atcagattga gaacgctgtc gcaatttcct acgaaaagct
    43081 gccgggcttc ccggtaagta cggtacatgg tcatgcgggg gagttagtgc tgggccatct
    43141 ccagggcgta ccggtggtat gtatgaaagg tcgcggacat ttctacgaag gtcgtggaat
    43201 gaccattatg actgacgcaa ttcgtacctt taagctgctg ggctgcgagc tactgttctg
    43261 caccaatgcg gcaggctcgc tgcgtccgga agtgggggca ggcagcctgg tcgcattgaa
    43321 agatcatatc aacaccatgc ccggtacgcc gatggtgggt cttaacgatg atcgttttgg
    43381 agagcgcttc ttctcactgg caaatgccta cgatgcggaa taccgcgcac tgttacaaaa
    43441 agtggcgaaa gaagagggtt tccctctgac ggagggcgtg ttcgtctcgt atccggggcc
    43501 gaatttcgag actgcggcgg aaattcgcat gatgcaaatt attggtgggg atgttgttgg
    43561 tatgtctgtg gtgcctgagg ttatttcagc tcgccattgc gaccttaaag tcgttgcggt
    43621 ctctgcgatt accaatatgg cggaaggttt aagcgatgtg aaattgtcac atgcgcaaac
    43681 gctggcggct gcggagctct ctaagcagaa cttcatcaac cttatttgtg gctttctgcg
    43741 caaaattgcc tgagaaaaac aataagaaac ggcctcgcgg gtgaggccaa atgatgcgac
    43801 aaggaaacga ttatgagcat cgcgatgcgc ttaaaggtaa tgtccttttt gcaatatttt
    43861 atctggggga gctggctggt taccctcggc tcttacatga ttaataccct tcatttcacc
    43921 ggcgctaatg ttggcatggt ttacagttcc aaagggatcg ccgcgattat tatgcctggt
    43981 ataatgggga tcatcgcaga caaatggctg cgcgcagaac gtgcatacat gctgtgtcac
    44041 ctggtgtgtg cgggcgtact tttttatgcg gcatccgtaa ctgatccgga tatgatgttt
    44101 tgggtgatgt tagtcaatgc gatggcgttt atgccgacta ttgcgttatc gaacagcgtc
    44161 tcttattcct gtcttgccca ggcagggctt gacccggtga ccgctttccc gcccattcgc
    44221 gtttttggta cggtggggtt cattgtcgcg atgtgggcag taagcctgct gcatctggaa
    44281 ttgagtagtc tgcagctgta tatcgcgtcc ggtgcgtcat tgctgctgtc ggcttatgcg
    44341 ctgactttgc cgaagattcc ggttgcggag aaaaaagcga ccacatcgct tgccagcaag
    44401 ctgggtctgg atgccttcgt gctgtttaaa aatccacgca tggccatctt tttcctcttt
    44461 gccatgatgc tgggtgcggt actgcaaatt accaacgttt ttggtaatcc gttcctacat
    44521 gatttcgccc gtaacccgga gtttgctgac agttttgtgg tgaaatatcc ctccatttta
    44581 ctgtcagttt cacagatggc agaagtgggc tttatactga ctatcccatt ctttttaaag
    44641 cgatttggca ttaaaaccgt catgctgatg agtatggtgg cctggacgct gcgctttggc
    44701 ttcttcgcct atggcgatcc gtcaacaacc ggatttattt tgctgctgct gtcgatgatt
    44761 gtttatggct gtgcattcga tttcttcaat atttctggtt cggtatttgt cgaacaggaa
    44821 gttgattcca gcattcgtgc cagcgcgcag gggctcttta tgaccatggt aaatggtgtc
    44881 ggcgcatggg ttggctcgat tctgagtggc atggcagtag attacttttc ggtggatggc
    44941 gtaaaagact ggcaaactat ctggctggtg tttgcaggat atgctctttt tctcgcagtg
    45001 atatttttct ttgggtttaa atataatcat gaccctgaaa agataaagca tcgagcggtg
    45061 gctcattaaa aggattcgca gctctgctct tcagagctgc ttttatgata aaggttaatt
    45121 agtgaaagat attttatttt cttccggcgt tggttttggc attggtgctt tgtttacaat
    45181 cgtgcgcttg cctattcctg taccaaatgt attaccaggt atattatcca ttgtgtttat
    45241 gtatgtcgga tatctggtgg tgaaatactt tatgccatga taatttaata cgatgtattt
    45301 attatatgga gcacttaatt atggaacgcg tatacagaac agatcttaag ttgctccgtt
    45361 attttcttgc cgtagcggaa gagttgcatt ttggccgcgc agcagcgcgt ttaaatatgt
    45421 ctcagcctcc gctcagtatt catattaaag agctggaaaa tcaactcggc acgcagcttt
    45481 ttattcgcca ttcgcgcagc gtcgtactga cacacgcggg caaaatcttg atggaagaat
    45541 cgcgtcgatt gctggtgaat gctaataatg tattggctcg ggttgaacaa ataggtcggg
    45601 gagaagcagg gcggattgaa ctcggcgtag tgggaacggc tatgtgggga cggatgcgcc
    45661 cggttatgcg gcgattcctc agggaaaatc ctaacgttga agttcttttt cgcgaaaaga
    45721 tgcccgcgat gcaaatggcc ctgctggaac gacgcgaact cgatgccggg atctggcgaa
    45781 tggcgacaga accaccgaca ggttttacca gcttacggtt gcatgaatcg gcgtttctgg
    45841 tggcgatgcc tgaagagcat catctctcat cattttccac cgttccgctg gaagcgctac
    45901 gtgacgagta ttttgttaca atgccgcccg tgtacactga ctgggatttt ttgcagcgag
    45961 tttgccagca ggtgggattt tcaccggttg ttattcgcga agttaatgaa ccgcaaacgg
    46021 tactcgccat ggtcagtatg ggtattggta tcacattgat agcggacagc tacgcacaaa
    46081 tgaactggcc aggtgtcatt ttccgtccgc tcaaacagcg catccctgca gatttatata
    46141 ttgtttatga aacacagcag gtgacgcccg cgatggttaa gctgttggct gcattgacac
    46201 agtaggtgag gagacaggaa ttgccggaaa tagtcgtctg ctaagtgaag cgcatcaggc
    46261 aatttcgcaa atcgcagata gcaaaaaagc gcctttaggg cgctttttta cattggtggg
    46321 tcgtgcagga tgactcgctt cgctcgccct tcgggccgtc gccgcgagcg gctccgttgt
    46381 ttcgctgcgc tcaactcgaa cctgcggcag cttcgaatct ttgagtatcg cagatagcaa
    46441 aaaagcgcct ttagggcgct ttttttacat tggtgggtcg tgcaggattc gaacctgcga
    46501 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccgaag tggtgggtga
    46561 tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca gctgagctaa
    46621 tcacccactt cggtacttca catttttaca aga
//
LOCUS       DAZGOB010000023        46059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGOB010000023 DAZGOB010000000
VERSION     DAZGOB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.023"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(232..1686)
                     /gene="amn"
                     /locus_tag="ACHLIF_001373"
     CDS             complement(232..1686)
                     /gene="amn"
                     /locus_tag="ACHLIF_001373"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO9605419.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(1788..3104)
                     /gene="shiA"
                     /locus_tag="ACHLIF_001374"
     CDS             complement(1788..3104)
                     /gene="shiA"
                     /locus_tag="ACHLIF_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO9605420.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFARLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            3419..4471
                     /locus_tag="ACHLIF_001375"
     CDS             3419..4471
                     /locus_tag="ACHLIF_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO9605421.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRTPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLDGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            complement(4641..6763)
                     /locus_tag="ACHLIF_001376"
                     /pseudo
     CDS             complement(4641..6763)
                     /locus_tag="ACHLIF_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(6690..7439)
                     /locus_tag="ACHLIF_001377"
     CDS             complement(6690..7439)
                     /locus_tag="ACHLIF_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO9605422.1"
                     /translation="MSKKYQLLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLLVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQTSGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            complement(8089..10110)
                     /gene="fyuA"
                     /locus_tag="ACHLIF_001378"
     CDS             complement(8089..10110)
                     /gene="fyuA"
                     /locus_tag="ACHLIF_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFO9605423.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIEDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPDGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
     gene            complement(10241..11806)
                     /gene="ybtE"
                     /locus_tag="ACHLIF_001379"
     CDS             complement(10241..11806)
                     /gene="ybtE"
                     /locus_tag="ACHLIF_001379"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFO9605424.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHN
                     FPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWE
                     DSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILH
                     SQGRPLSPLDEICIVDQDENDVAPGETGQLLTRGPYTISGYYHAPAHNAQAFTAQGFY
                     RTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTL
                     LGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRL
                     TALAVDRYRHSAQ"
     gene            complement(11810..12613)
                     /gene="ybtT"
                     /locus_tag="ACHLIF_001380"
     CDS             complement(11810..12613)
                     /gene="ybtT"
                     /locus_tag="ACHLIF_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFO9605425.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            complement(12610..13710)
                     /gene="ybtU"
                     /locus_tag="ACHLIF_001381"
     CDS             complement(12610..13710)
                     /gene="ybtU"
                     /locus_tag="ACHLIF_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFO9605426.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDMVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLYVADHQQNPHSLWRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPEACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            complement(13707..23189)
                     /gene="irp1"
                     /locus_tag="ACHLIF_001382"
                     /pseudo
     CDS             complement(13707..23189)
                     /gene="irp1"
                     /locus_tag="ACHLIF_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
     gene            complement(23277..29384)
                     /gene="irp2"
                     /locus_tag="ACHLIF_001383"
     CDS             complement(23277..29384)
                     /gene="irp2"
                     /locus_tag="ACHLIF_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFO9605427.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAYWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            complement(29575..30534)
                     /gene="ybtA"
                     /locus_tag="ACHLIF_001384"
     CDS             complement(29575..30534)
                     /gene="ybtA"
                     /locus_tag="ACHLIF_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFO9605428.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHDGFNHEQKPSTLWLQGQSLVMLSLVLDEQREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            30791..32503
                     /gene="ybtP"
                     /locus_tag="ACHLIF_001385"
     CDS             30791..32503
                     /gene="ybtP"
                     /locus_tag="ACHLIF_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFO9605429.1"
                     /translation="MALAGLAALTSLGALLFLVWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLQTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSEQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFVDTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            32490..34292
                     /gene="ybtQ"
                     /locus_tag="ACHLIF_001386"
     CDS             32490..34292
                     /gene="ybtQ"
                     /locus_tag="ACHLIF_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFO9605430.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIACPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            34285..35565
                     /gene="ybtX"
                     /locus_tag="ACHLIF_001387"
     CDS             34285..35565
                     /gene="ybtX"
                     /locus_tag="ACHLIF_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFO9605431.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            35593..36897
                     /gene="ybtS"
                     /locus_tag="ACHLIF_001388"
     CDS             35593..36897
                     /gene="ybtS"
                     /locus_tag="ACHLIF_001388"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFO9605432.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(37091..38006)
                     /locus_tag="ACHLIF_001389"
                     /pseudo
     CDS             complement(37091..38006)
                     /locus_tag="ACHLIF_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(38168..38243)
                     /locus_tag="ACHLIF_001390"
     tRNA            complement(38168..38243)
                     /locus_tag="ACHLIF_001390"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38208..38210),aa:Asn,seq:gtt)
     gene            complement(38344..39141)
                     /gene="mtfA"
                     /locus_tag="ACHLIF_001391"
     CDS             complement(38344..39141)
                     /gene="mtfA"
                     /locus_tag="ACHLIF_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO9605433.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            39235..39324
                     /locus_tag="ACHLIF_001392"
     tRNA            39235..39324
                     /locus_tag="ACHLIF_001392"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39269..39271,aa:Ser,seq:cga)
     gene            complement(39993..40643)
                     /gene="zinT"
                     /locus_tag="ACHLIF_001393"
     CDS             complement(39993..40643)
                     /gene="zinT"
                     /locus_tag="ACHLIF_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO9605434.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPIFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(40900..41535)
                     /gene="msrQ"
                     /locus_tag="ACHLIF_001394"
     CDS             complement(40900..41535)
                     /gene="msrQ"
                     /locus_tag="ACHLIF_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO9605435.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
     gene            complement(41536..42540)
                     /gene="msrP"
                     /locus_tag="ACHLIF_001395"
     CDS             complement(41536..42540)
                     /gene="msrP"
                     /locus_tag="ACHLIF_001395"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO9605436.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(42649..43062)
                     /gene="hiuH"
                     /locus_tag="ACHLIF_001396"
     CDS             complement(42649..43062)
                     /gene="hiuH"
                     /locus_tag="ACHLIF_001396"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO9605437.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            43195..43866
                     /gene="hprR"
                     /locus_tag="ACHLIF_001397"
     CDS             43195..43866
                     /gene="hprR"
                     /locus_tag="ACHLIF_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO9605438.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            43866..45224
                     /gene="hprS"
                     /locus_tag="ACHLIF_001398"
     CDS             43866..45224
                     /gene="hprS"
                     /locus_tag="ACHLIF_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO9605439.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(45332..>46059)
                     /gene="hchA"
                     /locus_tag="ACHLIF_001399"
     CDS             complement(45332..>46059)
                     /gene="hchA"
                     /locus_tag="ACHLIF_001399"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO9605440.1"
                     /translation="KPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLH
                     AAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKIADVVVSLNADS
                     EYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLN
                     GYSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTG
                     DTPFAANALGKLAAQEMLAAYAG"
BASE COUNT        10517 a        11892 c        12838 g        10812 t
ORIGIN      
        1 ttctaaagtc ataatcttat atttaatttt ataaaaactt ataatgttgg tggggttttt
       61 tataattgtg ttttgaaagc atacttgctt ttaatatgtg gaaatcatta agacgataat
      121 aaaactcgta tccgcccaag gaagcaaaca gcccgcgttt attgcgggct atttgccaga
      181 tgtcttactt aatccttcac atctattttt gataagaagg ttcagaactt attatcggaa
      241 cggcggctca ttaaaggttc gtaatttacg tgaatgcagt cggtcgcctt ctgcgcgcag
      301 caaatcgatt gcccgaatgc caatttgcag gtgttcggaa atagcgcctt cataaaaacg
      361 gttagcctgg ccgggaagtt taatctcgcc atgcaacggt ttatctgaaa cacacagtag
      421 tgtcccgtat ggcacgcgga aacgatatcc ttgcgcggca atggttgcac tttccatatc
      481 aattgccacg gcccggctta agttaaaacg aagtgcagaa gctgagtaac gtaattccca
      541 gttcctgtca tctgtggtta ccacagtacc tgtacgcagc cgctgtttga cttcctcacc
      601 gggcctgccg ctgactaact tggtagcgtc ataaagcgca cgttgcactt cagcaatgct
      661 tggaatagga atatcgggcg gcagaaccgc atcaagaacg tgatcatcgc gtaaataagc
      721 atgtgcaagt acataatcgc caatggcctg gctttcacgt aagccaccac agtgaccaat
      781 catcaaccag acatccgggc gtagtactgc cagatgatcg cagatggttt tagcatttga
      841 cggtcccacg ccaatattca ccagagtaat accctgacca tcggcggtaa ttaaatgcca
      901 cgcgggcatc tggtgttttt tccatgcaag gtcggaaatg gcttcttctg gcgcttcggt
      961 ttcggcggtg atccagttcc cgccagcaca agaaagggca atgtagggac tatcaggatc
     1021 gaggatctgg ctgcatcccc aacgaacgaa ttcatccaca taacgtgtgt agttggtaaa
     1081 caagacgaac ggctgaaaat gttcaactgg cgtaccggta taatggcgca accgcgcgag
     1141 ggaaaaatcg acgcggcgcg catcaaaatg cgatagcggg gagaattcag ttggatgata
     1201 aatgccgtct gcagtttcat cgccaatttg cgccagttct gttgtcggga aatagcgagt
     1261 taacccagcg ctcattgagc gatcaagtgt caattcagag ccatcaatga cataaggata
     1321 agggatttca tgctgcgagg gttgcaccga gatatgcgca ccataatcct gatacagcaa
     1381 cgtaagttgt tcattgagat acgaacgaaa gagagtaggg cgagtaatcg tggtggtgta
     1441 gctgcctgcg tgagtaaaac gaccaaatgc gcgcgtttta gggggattgg ttgtgctacc
     1501 gtcccaggtt acggtcagtg atggatagac aaaaagacct tgtttgcggg cgttttcatc
     1561 aggtaattcg ccacttgtaa tatagttgcc aatggcgttg cgtaaagcaa ctacagattg
     1621 ctcatacagc gcgtcgagtt tatccagtgc ctgagctggg gtcagaccgg agcccttatt
     1681 attcatgtgt gtctcctgtt ccatacaatt accgcacatc cgatagtatg tcacaggaat
     1741 gtgaaacaaa agtgtaaggc gaagatcgga tgatcttcgc caggctatca agcgcgttga
     1801 ttgtctttca tcaacaaagc ggttattgcg gaaatcaggc atccagccag caaataaatg
     1861 gcgacgctat gccagttccc ggcaaagtaa gtgatgagtg cagcggcaat aaaaggtgta
     1921 aatccaccac caaccacact ggcaacctga taaccgactc cagcgccact atagcgataa
     1981 ctggcaccaa acatttcggt aaacatcggt tgttgcacac acaccaccat gtcatgcgca
     2041 atgtttgcca gcattatgga gaagaaaact atccagaaaa tagattgtgc ttcaagcgcc
     2101 ataaagaaag gaaatgcgct caacattccg attaacgcac ctgtgatata aaccctgcgg
     2161 cgaccaaaac gatcggcaag cctggcaaaa cagggaattg tcaggcagct taatccacct
     2221 accagcaaac caatattaag gaaaagttcg cgcggtaacc ccatattctg ggttgaataa
     2281 ttaagtgcaa aggcggtaac gatgtacatc gtcagcaact cgcacagtcg tagcgcaata
     2341 atcttcagga aagcaccggg atgtcgtaac agagcttcga taaccgggat gcgttttttc
     2401 gcggccgctt gattatgttg ctgttgttca aattccgcgg actcctccat gccattgcgc
     2461 acccacaatg ctcccagaac cagtacgatg ctaaacagga aaggaatgcg ccagccccag
     2521 cttaaaaact gtacgtcagt cgtcatcata ctgatcaatg aaaccagtcc ggttgaaagc
     2581 agtaaaccta ctccgtagcc aacttgtaca ccgctactgt aaaaggcttt tttatttttc
     2641 ggcgcacttt caacggaaag caacgctgcg cctccccatt cgccgccgac ggcaaatccc
     2701 tgaatggcac gcagtgtcac cagcaaaata ggtgcccacc acccaatggt cgagaatgaa
     2761 ggaagaatac caatcaaggc tgtcgcgatg cccatcatcc agacggtcag cattaacatg
     2821 cgcttacgtc ccagccggtc accaaagtga ccgaaaatga caccgccgag cggtcggaaa
     2881 aggaagccga caccaaaggt ggcaaatgcg gcgagcgttc ccatcgccgg gcttacttgc
     2941 gggaaaaact cgcgattaaa caccagtgcg gcggtaatgc catagagtaa aaaatcatac
     3001 cagtcgacga cggcaccagc gaaactgcct aacgcagctc gtcgggcgcg acttaacgaa
     3061 agcgtccctt catcgggacg agtggagatg agcgtggagt ccataatgat cctgtttgta
     3121 ctgtcttttt ataattagta tggaaaaggt aactcacatt gccgtcgatc tataaacgaa
     3181 ccttatgaga ctacctcgac agagcgcatc tgcaaatgat tttatggctg attagcggca
     3241 acatgaaaaa ttcgatggaa gataaaagcg caggagttgc tggtgttgtt taaggattaa
     3301 gcagagggcc cacaggttcg agatatgaaa cataaaagtc cgcatatgca caaaattgca
     3361 ccctgatgat tctgatagac tgcattgcat tataaccagt agggaggcga ggttagttat
     3421 gtttttagta tcattgttga gacgtattgc atttagttac tacgattata aagcttataa
     3481 ttttaatatt gaaaaaacag actttgttgt catacatatt cccgatcaga ttggcgatgc
     3541 tatggccatc tttcctgtta ttcgggcgct tgaattgcat aaaattaagc atctattaat
     3601 tgttacgtcg acaattaatt tagaagtctt taatgcgctc aaacttgaac agactaaatt
     3661 aacattagtc acaatgacta tgcaggatca cgcaacatta aaagaaataa aagatttagc
     3721 gaagaatata acactgcaat acggtacgcc ggatctttgt atagaggcga tgcgtaaaaa
     3781 gaacctgaaa acgatgatat ttatcagtca gttgaaagca aaaacgaatt tccaggtcgt
     3841 cggtttaacc atgaagtgct attccccttt gtgtaagaac gcgtcccgga tggatcagaa
     3901 tctccggact cctgtaccca tgacatgggc atttatgatg cgtgaggcgg gttttccggc
     3961 agtcaggtca atatatgaat tgccattaag cgatgatgta ctcgatgagg tacgcgagga
     4021 aatgcgatcg ttaggatctt acattgcgct caatttagac ggtagctcgc aggaacgtac
     4081 attttcatta tcgattgcag aaaatctaat agcaaaaatt caaagtgaaa cagatattcc
     4141 aatagtgatc gtttatggac ccaaagggga agataaagcc agggcattag tggattgtta
     4201 taataatgtc taccgtttat ccttatcacc ctcgattaaa cgttcagcag caatcataaa
     4261 agatgcttat attgcaataa ctcctgacac ctcaatatta catatggcaa gtgcctataa
     4321 tacccctgtt gttgcaattt atgctgatta caaaacgcga tggcccgcaa tggccgatgt
     4381 ttcggagtca gtcgtcgttg ggcaaaaaat tgacaatata agtctggatg aattcgcaaa
     4441 ggcattaaaa agtgttttgg cgagaatttg aggcagtact tatgcatagt aatgccgatc
     4501 tgttatgaac ggtggtgtca tcaatactct gaggctggtc aatcatcaca gaaaaccagc
     4561 ctttggatca aagcaatact caccgaaaat gatcatagtc acaccgaacg cgattcagga
     4621 ggaactgttt ggtaaatagc accaccggat cttgggggcg ctgtgattat tactgtattg
     4681 ctatcaccgt catttttgaa tgtcacttta ccagtagcat caacgcctac ccaggaggcc
     4741 gagcttgaaa acgaataatc agccgccgtt tttcctggtg caaagttgtc attattcaac
     4801 tgataatacg ccccggtaaa tccctgcgaa gggaatgaag ctgtcggtag gtttgcacta
     4861 ttcactgaca ctgtacccgt cattggtctg gtttccgcgc taatgaattc tatcgtggta
     4921 ctcaggcccg cctgatgaac accattcaat acggggatca gcgttgcgat accctctccg
     4981 tctcctgtaa cagtagcttt gtaatcacca ttgatatttt gactgtaatc aattgcgcta
     5041 attttcatat agggcacgtt tgtacctgat tgcacaaatt ccatcccttc agaaactttg
     5101 atcggattgc ctgatatatc gtgcagaaca agatgtagct cagcactatc ggtaaagtcc
     5161 cctttcagtg atgactggtt gttcttaagg atggactgcg aggcgtctgc tgggcctgcc
     5221 actagcgata tatctactgt ttgcattcca cctgcgctta tgactgcgct tacagtgacg
     5281 ctccctgtta tcgctcatct cacgtttgtt gtcgcgattc cgttttgatc ggtcaccgct
     5341 gttaatgacg ttaatgtggt ggagccgctt tttaaggcga agtacacagt gagaccttcg
     5401 atcaggttac cactgccatc ctcaaccgtt gcctttaagg tacttaaatc actgttggtg
     5461 gcggcgatag tcgacggatc agcgataaag ctagcaacat gtgcggtgct tgggttgcca
     5521 gtaaccttca cggttgtctg tgtctgtatt gtgacgccgt tatggaacga tgcagtcacc
     5581 gtatatgtac cgactttatt gctggtcaga acgactggag cctgacctga agagttggtt
     5641 ctcacttttc cgccacttaa cgtcgcacct tctagcgtta cgctaaagtt gatgacctga
     5701 ccctccactg gagtgccatt tgcagaggtt agcgttgccg tcagagtagc gcctttagga
     5761 gcattgacac cgataagcgg actggacgtg agtgtcagtt tttcatcaat agcctccagt
     5821 tgtttctcaa gtgaagctcc attcgctagg gatgctttta cagtatacga gccgtttctt
     5881 tcagacgtca tggtgacctc ggctactccc tgcgtattag tagagaccgt gttctggctg
     5941 atgatcattt gcgaggatgg cgctgcactg aatgtcgcgg cttgatgcgt caccgggttg
     6001 ccaaactggt cgttaacgtg agctttaact gctatgtctt gtgcgaccat gacctgacct
     6061 tcaggtatct cctgactggt aatcgtcgca gaattaacat ctaccttggc attcagtaat
     6121 cgcgagatga cttcagtagg tttatctgcc agagaggctg aaactgtttt tagtccgacc
     6181 tcggtgcttg tcacaagaat ttcagcaaaa acctgatcat ccgtttcaac gctggtgctg
     6241 cttagcgtga cggaggttcc acggaaatta acttttatac cttctactgg gttgccgttt
     6301 ccatcagtca ctattgcttg tagcctggtc attccaatgt tattggcgat gaaattgtcc
     6361 tcggtaacgt taagattaac ctgcgcagtg agcgtatccg cagtaaagtt caccactaac
     6421 tgctcactct taccgccaac catcgatgct gtcacggtat gagcgcctgc ttttgtacct
     6481 ttcagcgtga ctttcgcttt gccatcggca tcagtaaccg ctttgtcgcc atcgctcagc
     6541 gtgaagttcg ccttcacatc ttccggtaga gtaaatgtta cctcagtgtt ggctatcgca
     6601 ttgccctctg tatcagcgac ggtgctgtta gtgtcgtgag atcgttattg tcggcaatca
     6661 ccggatcctt caaggctgcc agcgtgattt cagcattcgt gacgttcggc acatagttca
     6721 ctgtttgttg catcgaatcg ccattttcga gggttgccgt cacgggagtt cttcaggctg
     6781 aaatcttcat ccacgcccag cgtgattctt gcggtaccga agcggcttaa ccagtctgtc
     6841 attacccctg aagtctgaga agaggcccat ccacgtgcta tgtttgccgc ttgctcgctg
     6901 ttcatatctt cggcgagcag agatccaatt agctgtgaag tactggctat ctgttgctca
     6961 aggttgccac tgctgttacc cggcggcggg gttaaatttt tttcactaac ttgtgcctgg
     7021 acatccagtt catcacccgg gcggacatta cgaacaaacg tacgaaactg gttgagtttg
     7081 cgtaacaggg gggtatctat aatttgttgc acaggacaaa tatatctggc ttctgtacag
     7141 agtcttaaat tttgaccata aataaaaccg tccctgtcaa gcatgcatag tttatcaagt
     7201 aggtctccca caaactgcct gaattttatt tttccattaa aagtttgtat cttcttaagt
     7261 atcacaggag actggaaata gaccaatagt aggatgttgc cactcaaggc atgacgataa
     7321 atatcgctag gagtaatttt ttgttttatg gctttggtag tgatctcaac tgcttccgtt
     7381 atagtgaccc atgttgacat ataatgagtt ataagcaata gctgatattt ttttgacata
     7441 atattatctc cttctacaca acaattcgtt ccgttaagga aaaagattgg tgtcacaagt
     7501 atttatttat gattaatcag agggtttatg acagagaaaa ggatgtatat ttctgagtaa
     7561 acatgtacag gcctatgggg tgactattta atgagtaaat gccgaatgat tttcggggtg
     7621 atgattataa cttgaggaaa aatcccagcg tggtttgagt agtgtacctg agtgatattt
     7681 gtgttatgta tgcattgatt gcagcagaat aaatatagtt caggctagta tagtttatgg
     7741 tagctatgag aatatataat ctattttacc tataaaataa tttatatgta tggggttgtt
     7801 ttttaggttg tttgtctgtg cttttatatt ctggagatgt gccttgtata taggattgtt
     7861 tgttggttgt attttttaat gttgcactgt tgtgaatttt tattttgata tgattatttc
     7921 tttagtataa ggagagaaag gaacgagaaa gttaaatcta tttatcctaa tcgcaatttc
     7981 attttttaaa taatcactat ggattaatat tttttgcgac tctgctgagt tactggtttt
     8041 aactagtggt gtaaaaggga taccttttcg gtatcccttt tacaataatc agaagaaatc
     8101 aattcgcgta ttgataccga cggtgcgacc catattgacc tgcgcgacgg cgctgctgcc
     8161 gttcatgtag ccataggtac ggtaacgacg gtcgaacagg ttatcgacat agacggaaat
     8221 gttcatccgt tcagtcgcct gccagcccag gctgctgtcc agggtggcat aggtgccttg
     8281 ccgcaactgg ttgtcgccat cgaaataatg cggcccgacc agattaaccg ccagtcgggg
     8341 catcagtgcg ccatagcgcg tatcaatcac gccgttcacg ctgcttcccg cgccataacg
     8401 tggtacgaac ggcacccggt taccgtgata caactcactg tcattggtga attcggaacg
     8461 gatcacgttg ccattgatat cccatgacca gcctggcgca aaccgccact tcgcttcaag
     8521 ctcaacgccg gtggcgtcgg ctttacccgc attgcttaat gtctgcatcc cgtccgggcc
     8581 agagtaaagc tgcatgtctt tggtgtgggt ataaaacgtc gcggcttgca gcgtgacgtc
     8641 agcggtttcg tagcgggtgc caagttcata gttgatggat ttctcggcga cgaacggttt
     8701 ggcatcaaga cccgcagtag gcacgatgtt gtacccggaa ggtttatatc cctgggctac
     8761 acgggtatac actctccagt catcggtcag catatagcct gcggatagct gcccgagcac
     8821 ctgatcgtca ttgctcttac cctggtcgcc aaacgggttg ccgagcatgc tgccgtgata
     8881 ttgtgtactg gatttatcat gcgagaagcg cacgccgccg ccgatatcaa aacgatcggt
     8941 taaatgccag gtcaggtcac tgtatgcggc cagcgtttca gcggtggtat agccggtact
     9001 gcttaaataa ggcattgtcg gcatgtcgta ggctgaattt aacttctcgc gggtgttctg
     9061 ccggtacagc ccaaacacca tatcaacggt acgcgcatcg cccagggttg cggcgcgcag
     9121 ctcctgcaca tcctgattcc agcgctgagg catattgacg attaacgaac cggaagggaa
     9181 ggtgcgcgaa taatgctgct gctgccaggc gctgatcagg ttgaaaaccc agtcatcggt
     9241 ggtgtatttc ccactcaggg tttggctgtc agtgcagcgc cgcatgtacg ggtctggtga
     9301 accatcgctg atcgacagct tacggccctt aatatcattc catcccacat aggcatcctg
     9361 ggtggcgcga gtacattcgc gtgaggcggc aaagcccatt tcccagggct gatcgtccgg
     9421 cgccagacgc agtttcacat tccctatgct ggcgcgggtg ccgcctaagt catcgcttcc
     9481 cgtcgcgggg ttaatcatgt cgccgtcatc aacctggcgt aacagggtga cgctgccgta
     9541 cagcaggcca tcctcaatgg ggccgctcag gttgaactta cttcgataac tgtcgcggct
     9601 actgacgccg ccttcaatat agccgcgcgg cgtgctgtcc ggctgctggg tgacgatgtt
     9661 gatgatcccg ccctgagcgc ttttgccata taacgttccc tgtgggcctc gcagcaactc
     9721 cacgctttgc acatcggtaa gcgcctggat ggtgttggtg gaaagctgag ggacgccatc
     9781 gacatacagg gtgacggcgg ggttatagaa gtcctgcgct gaagagacgc cgcgtagcga
     9841 gatcgtcgaa aaaagcatgt tgccgctatt ttcaatattg agcccgggca agactctggg
     9901 gagtttgtcg ctggcggtga cgccggcgtc gcttaattcc ggcgcgctga caacagtaga
     9961 cgagacgttg ttggctgatg ccgagcggga agattgttta ctggcggtaa ccaccagcgt
    10021 gctttcgtct tgctgtgaag tctgggcatt agcaatggcg gggagcaata atccccccaa
    10081 ggccagagga taaagccgtg tcattttcat tgttgtgagt ccctgtaaga cggcgaaacg
    10141 ccaaaaatac cgacaatggt gaattgaaaa tgataactat ttctaatagt ggtgctaccc
    10201 gaaacgggtt tatttcaacc tgtttcgggt cggtttgcgc ttattgggca gaatggcgat
    10261 aacggtcgac ggcgagagcc gtcaggcgtt ttttgtctat cttgccgacg gcggtgagcg
    10321 gccagtggtc cagaaactcg atttggtcag gaattttcca cgcgctgagc cccatacggg
    10381 tcagttgttg acgcaactgc tgatagtcag ttggcacctg ctgcgcgatg ataaacgcgc
    10441 aaatccgctc gccaagcagc gtgtccggcg cggcgaccac cgcgcaatct tgcacttccg
    10501 ctaaacgcag cagtgccgat tccacttcag ccgcggctat tttttctccg gcgcggttga
    10561 tctgctcttt tatgcgtccc tcaacgtgca ggttccccac ctcatccagc ctgacattgt
    10621 cgcctgtgcg gtaaaaccct tgcgcggtaa aggcctgcgc gttgtgggca ggggcgtggt
    10681 aatagcccga aatggtataa gggccgcgcg ttaacaattg cccggtttcg cccggcgcga
    10741 cgtcgttctc gtcttgatca acgatgcaga tttcatccag aggggacaac gggcgcccct
    10801 ggctgtggag aatggtggca tgcggatcgt ccagccgggt aaaacagagc aggccttccg
    10861 ccataccgaa aacctgttgc agggtacagt caaaggtggc gataacctgc tcagcaagcg
    10921 tcgggtcgag ccgggcgccg cctgcctgaa tgacgcgcag cgacgaaagg tcgctgtctt
    10981 cccactccct ggcctgcacc cataattgcg ccagcgccgg aaccagggcg acgtgagtca
    11041 ctctttcctg cgcgattaaa ggcatcacct catcacagct ggcgctgtcg gtcagcacca
    11101 cttttccgcc gcaggcaagc gttcccagaa taccggggca ggccagcgga aagttatgcg
    11161 ccaccgggag gacggcgaga tacacgctct gttggctgat gccgcacagt tcagcagaag
    11221 cgctgaagtt atagctatag tcggcatgtc ggcgcgggat gagtttgggc gtgccggttg
    11281 tgccgcctga gagcaacaac aacgcggtgg tggaaacatc aggctgtcgc tcaccgtgaa
    11341 gggagaagag cggcgtaaaa tcgtcgctca tggtctctcc agcgaccaga acatgacgca
    11401 agcaggcgtg tttgtgtgcc atctgtcggg ccagctctgc gtggttttcc ccgtgaataa
    11461 cgtaagcgac gggttgcgcc agctcaatca gcgcgtcgat atccagcgcc cgttgcgagg
    11521 gcatcgccag cacggggata acgcccagtc ttaacaaggc gaacagcagg gtaacaaacg
    11581 cgttgtcgtt gggaagctgc acaattacat gctcccccga acgcaaaccc agtgatgaca
    11641 gacctgcggc aagttcgtca acttgtgcat caagcgcgct gtaggttaac gacccctttg
    11701 catcaacgac ggcaattcgc gaggcataac gggccgccca gtggcgcaac tgttcggcaa
    11761 tgggtaaggg atactgttca atcagagatt caaaggaaga attcatgcgt cacctttctg
    11821 aagtactggg ctgtttttgc cacgcggtca ttgcagaaaa tgcgtgggga aaatggcgga
    11881 caatctgcgt aaaaaaggac cgggcttgtt gaatgggata gaagtgatcg ccgtcaatca
    11941 ccaccgggcc tgtaacgtgg ctcagccact gacgccaggc atcgacctgc tgccaggagg
    12001 cttcgcgatc gtggctgccg cacaataaca gcgcaggcgt gcgcagcggc ggacagacgt
    12061 cgggcgagtc gtagtgatag ctctcggtgg cgtaaaaatc agcgcgcaga agaggaagaa
    12121 acagcgacat taattcctgg ttttcccgca gttcaggaga acatccgcca atgtctatca
    12181 gctcagcgat aaagtcggca tcatcgcgat ggctgagctg gcgttcagaa tgcagatgcg
    12241 gggcatggca cccggaaata atcagtcctt gtggcgcaag cccccgttgc tccagaagtc
    12301 ggcaggtttc aaacgccacc tgcgccccca tgctgtgccc ggcgagtaaa agcggcgtgt
    12361 caggcgatac ggatgcttcc agctcgttcg ccagcagtgc cgccagttgt gtaatgcttc
    12421 tgagcggttc cagatggcga aggcgatcgc gccccggcca ggtcaccaga gaaagcgcgc
    12481 aatcagccag ttgctcagct tgccagtggc gaaacgcgct actgctgccg ccagcgaaag
    12541 ggcacatgac cagatgcgca gtattgccgt tccgggcggg ccacagcggg atgcacattg
    12601 cagattgcgt cacagcgcct ccttatcatc atcgttgtag aaaccggcta accggtcgtg
    12661 gtgcggcggc gtcagacgtc tgatctccgc attgccggtc ttacgtaaaa tctgctgcca
    12721 taaacgtgat aacgcgatct gatgtacgct ttggcaggcc tctggtggat gttcgcccgc
    12781 cagattgctg cgcaactggt gtagcaacca gctgacgccc tccggcccga cggtttcgca
    12841 gcagtcgcgc cagctcaggg gcgccgcgct gcgcgtcaga cccggcagat cgcgcaggat
    12901 ctccggtctg cgccagagac tgtgggggtt ctgctgatgg tctgcgacat acagactgga
    12961 tgaccagatg acggggccgt agctcgcctc cagcgaaaga tgaccttccg gccagcccag
    13021 cagcaggcgg tgcatgataa ggctatgcat atctggatcg tcaggatcga gatagcgctg
    13081 aagcagcagg caggcttctc cctccggcca gaagagtctc agacagtgaa aatcgctaaa
    13141 gctgccaacc atgtcgcact ccacggcggc ggcatcaacg cccagagcca gcaacaacaa
    13201 atccagagtg gagtagagca actgtcggct ggtggtggcg tggactaccg gcggcgtctt
    13261 agccaggcag cgacgcagct gctgcgcatc gcgaagccat gtgcgtcccg cgcgcgtatg
    13321 cggataaaac gtgtttaccc aatagcagca gccctgttcc tgcgccagcg tttgtaagga
    13381 actgatgtca tccgggtgga ggggatgctc ctgaatgacg tgtacgccgc gcgtcagaaa
    13441 gtgtctggca agctgcgtac cggttcctcc ggcgacggtc gaacgcacga caatacaggc
    13501 gatatccggc atcctggtta tctgttccgg cgaggtatac agcggaatgc caaacgcatg
    13561 agccagctct cttgaacggg cgcttccctg cgccagcagg ccgaccagtt ccagcccctc
    13621 cgggggctgc ataaaggcat tcaggtacat ttcgccaaat ttggcgccca caatcagtac
    13681 gcgttgtttt ggggaggcgg acggcatcat aacgtgttct ccggttgcgt tgaggttgcg
    13741 caaagccagc gcgtaatgtg ttgcgcacaa gcctgaacgt ggggggcttc catcatgatt
    13801 tgccagtggc tggcttcaat cacagcgtcg tcggcgttgt ttatccagcc ctgcaactcg
    13861 gtttctgctg gcgtccagcg cgcgggtctc ccggcggcat acaccatgag acaggggacc
    13921 ggaacgcttt cgcccggcgt atgctcagtc agtaaacgca gcagatgcgc gatgttgtcg
    13981 agccacgttt ctgccgcttg cagcgtcagg ttttgcgaca ccataccggc cgttttagcg
    14041 aggctgataa agtcggcaag ctggctgtcg ggcgtctgct ggtcgaaatg ttcgggcagt
    14101 gccagaggcg tttctccttc ggctaacagg cgcagcaggg ccgcccggtt ttcgcaacag
    14161 aaatcctgtc ggcacacggg atcgattaac accatccgaa cctgcttgcc tttggcgtac
    14221 aggcgctgtg cggcgcccgc cgcaagaaac gcgccatacg accaacccgc cagcacataa
    14281 gggccgtgag gctgctgacg acggatgcac ccgacatact catcgatcat ctggtcgagc
    14341 gtagcaaagc gctgcggcga ttttgcttgc aggccgaaaa cgcgcctgtt caacgcgcta
    14401 gcgagcggca gccaggcgct gatatcgccg tctgaagcgt ggaagacaaa cagggtttcc
    14461 tcaccgtcgc cctggcaaag gggtatcggg ttgtcctctc ctgacgtagc cgcctgtaga
    14521 tgggcgcaga agtcgctcag cgtcgaatgg ttgaacagat cctgaaggtt agccctggca
    14581 atccctctcc ggtttagctg cgcgaccatc cttgtcgcca tcaggctgtc gccgcccagt
    14641 tcgaaaaagt cgtgatgcct tgccacgggg cgagacagca gcgattgcca gagttcagcc
    14701 acctgttttt ccagcgccga attccccccg gcaactggcg tgggtttggc tggctcagtc
    14761 ggcgcatcag acggggcatg cagcggcgta aataacgctt ctctttgccg gatctgtaaa
    14821 atgggcagac cggtgccaaa gcgctgctgt aaatagcggc tcacggcttt tttatccggg
    14881 cgatttacgc caggcgaacg cgctaccagc agatgctggc gcagcggcga cgattcctgc
    14941 gcgggccacg ccagctcgtt tgcaaacccg gcctgaacga gaacctcctg ccatgcggag
    15001 cgggtgagca tcggtttctc atcccggcgg cggaaatcgc ggtatccgct taatccctca
    15061 ataaagccca cgctcgccag ctggaatacg ctgtttcgct ccgtcgcttc aacgatcagc
    15121 aaacgtccgc cggctttcag caacagtttt aatctgcgca acgtctggac gacatggctg
    15181 gcgtcgtgga gcacattgac ggcaacgatc aggtcgtaac ccgcctccgg gtgggcggtg
    15241 aaatccagcg gctggttgat gtcgaacaag gcataagaca cctgcgattc atcatggaaa
    15301 cgggctctgg cgtcattgag gaactgcgcg gagacgtccg tgaagtggta cgactgccgc
    15361 gtggccttca gcaccggcgc ggtagtggct gcggttccgg cgccaacctc cagaatccgt
    15421 tctgcgctgc acaaggcggc aatctgcgcg gtatagcgat tcagacaggc gctggcgggg
    15481 ttgtcgcgat acagcgcgtc ggtaacgcga tgctgctcgt tgaacagcaa ttccagcgga
    15541 gaacactgcc cggagaagag ggcgtcgtgc cgggcgatgc aggtttccag atactgcgcc
    15601 agcgcctggc tccattggct ttgcgggcac gcttcctgag gctcaggaat ctcgctgagc
    15661 ggaatgcggc agcgccagct ttcaccttcg cggattaacc actctctttc cgtcagacac
    15721 tgtagccact ggcgcagcag acgctggtga gacgcttgcg cggacagcgc ctgtactatt
    15781 gcgctaaagc gatgggcgat ctccggcgtg gtaaacaggc cgtgacgatt tagcgtagcc
    15841 gcgatcccct gtagcgcacg cgcctccagc cagcgccagg tcgcctccag ctcatcaagc
    15901 tgcgcctgct caggcaacgg cagcgccagt tgggtaagtt cgtgctcatc aggcatcgcc
    15961 gctgatggcg aaggatcgtc ggccatcacg atatccagcg tcaacgcgcc ctgttcggtt
    16021 atttccgctc tggcctgccg gatgccgggg tattcgagcg tcgcctgctc aatatcccgc
    16081 aggtcgtagt ccgcctgcgc gttcagcggc cagcgtcggc ggggaatgtg cccggcgatg
    16141 ccgctggcga gagtcttcag gctttcaggg gcggcgatga cggcttgcag gatggctcaa
    16201 tagtcattaa acatcgcctc ggcagcgccg ggttccagca cgttgtccat gcagtaccag
    16261 ctaaacatca actcgccgtc gctctccatg acctgatgat ccagccagac ctgcggcgtt
    16321 tgcgtgaata catagcaggg ttcgccgaac agatggctca tcgcctgatc gatagtcatg
    16381 ccttccagcg tcatccccag catactggta aacactaccg gcatcagcgg ttgacgttgt
    16441 gatccgcgca ggcggcccag ctcacggatc acctcaacac cgttcatttc actgtgcgcc
    16501 atattttgcc agaggcgctg ttgggtctgt tgcatctgct cttgcaacgt cactggcgct
    16561 gagaagttaa aatcaaccag cgtgacggag gtaaaatcac caatcaactg gttgatttgc
    16621 ggatggatcg gctggcgatt gaagaacgtc aggttcagcg taaatgttgt ggtacggctc
    16681 cagcgctcaa gggtggcggc aaacagcgtg agcagcgcgg cagacggtgt gacgccttgc
    16741 tgctgccagc gctgtttcac ggcctgccat tctgtcttgc cgatcgtcga tttgaaggtg
    16801 gtgaagtgtg gcgtttccgg gggcgtctca accatcggca gctctggcgc taagggcagt
    16861 tgcggcagtt tttcctgcca gtaatcccag gcatcgtgcc atgccgatgt ctttcggcgc
    16921 gcctgttcag ccatcacata atcacggaag gtaatagcga gcggcgccag cgtttcaccg
    16981 cgccagacct gcgccaggtc gtccatcatg actttaaaac tctgcacatc aaactgcaaa
    17041 aggtcgaggt tcatatgcag acggtaatgg caatcgtcga tttcgctgac caccagctca
    17101 aaaagaggcc actggtcggc aggcaaaacg cgatagctca gttcatgccg ccgtttttcc
    17161 agcgcgatgc gctgttcttc cggggaaagc gcgcgcagat cgtcacgcgg gatgtgatac
    17221 tccggcgttg tcgccaggat tcgctgctgc ccgtcggcat caaccaccat acgcaacata
    17281 tcgtggcgtg cgatgagctg gttccatgct ttctccagta tggctagatc gaactcatcg
    17341 tggcgtttat cccactcaaa caggacgtga caggcgacgc cgccatagcc aatgaggtgg
    17401 gttcgcccca gccagtaggc gtgctgaatg ggcgtcaaag ggaagggcgc ataacgctcg
    17461 tcggcgtcgt gccgcaacac ttccggctgc gaaacggcag gcgtcgcctc tggcttagaa
    17521 cagataagct gcgtgagtcc atgaggagac agatcctgcc acgcccgttc cgcattgatg
    17581 cgtacgccca ggtagtgctg aatatcgcta ctgagttcaa ggaagagcag cgagtccatg
    17641 ccgagttgca acagatcctg gtttggatgc agtgacgccg gatcgctcag ccttagctga
    17701 accgcaatcc gctttttcag ccatgccatc accgctgttt catcgctcag gctgccgtta
    17761 aaggcgttgt catcagcagg aggaatgacc ggcgttgcgg ctttttctgt ggcgctgatg
    17821 ttaaacagag cctgttgtaa cgggggcatt ttgtcggtaa aaacgcgcat cgccagtcgc
    17881 cacggggcgc cgcgcatcac cgcctgttcc aggtgccagc agccttcggc atcgcttaac
    17941 gcgcccatac ctcggctggc gagcgtcgcc agcatttccg gcgtggccgc ccgaccgctt
    18001 tctccccatg cgccccaggc gacagagagc gttttcggcg catcaagggt ggaaaactgc
    18061 tgggccagcc cgtccaggta gccgcaggac agcgcatggg cgctctgacc cggcgcgccg
    18121 agggtggcgg cagcggaaga gtagagaata agatagcgtc cgtcgtggtt gcgcagggtt
    18181 tgcaacagct ggcttgccgc ctgcgctttt accgcgaaaa cggcagccag ctggtggtca
    18241 tcaagctcct gcaagggcgc gtcagccaat acgccagcgg catgaatcgc tccggcaatg
    18301 ccgccgttgg ccgccagatc gtcaagaacc gtggccagtt gcccggcatc gcccacatca
    18361 caacggcaga cgcgcgtctg cccgccctcc acgtcgcgta gccatgactc atccacgtgc
    18421 gatgccagca gggcgatgcg tcgcgcccct ttttctctga gccagttcac ggcaaggcgg
    18481 cctaatccgc caaacgctcc ggtcaccaga tgccagcggc tatcgccggt aaacacgttt
    18541 gccggtaatt cagctgcgca tcccgtattg ggcgccagtg aagggagcca aagggtgtca
    18601 ccccgtgcgg cgagccagcg ctgtgatagt gagactgcgc tcaacccttg atgcagcgtt
    18661 tcccacgggg tgttttcggc gagatcgatg gcggcaagca accgttccgg ctgttcgttg
    18721 gccgcgacgc gaagcaaggc ccatagcgcg tgatgggatg cagagagtgc ttcattttcc
    18781 tcgactcgcc acgcgcggcg agtaaccaca atcaatccgg ctgcgctggc ggtgagcgct
    18841 gctatcactt tctccgctaa ggccagcgta tcctcgctct cctcaacaat catcagtcgt
    18901 gaagaggcgt gcggatcgtg aatgatgccg tattgcgcca gctcgtcgct gcgcgcaagc
    18961 gtaccggcgc tgaagctaaa ggtaagcgga tggtcaatgc tggcgacgtt gaggggcgtc
    19021 cagcgccatt gataatgcgt ttgcggcgca ggaagaggcg cggcgggcga tagcggcagc
    19081 cactcgccag caggatgacg ggcttcaacg cgcatctgac cgcgggaaaa ggcttcgttt
    19141 tgccagctta agcgaacctt ccccagccac tccggggcgg cgacgggggg cggttccaca
    19201 ggcagagcgt cgccatgacg ctgtgaaagc agacgccagg cctcctgcca gcgggcgtta
    19261 aactgttcgg gctgacctgc gcaatcagac cagtcggcga gataatcacc gttatccgtc
    19321 agcgcctgcc ccaacacggg ttctgatggc gcggtgaatg ttacggcgct aaccgcctga
    19381 ccgggcagcg tggcgagaat gatatgttcg ctcatcccgg cggtcgggct gccatcctgc
    19441 ggtagccacg ccactttgct gaatccggcg tggcggcact gttgttgcca ctgagcggtg
    19501 gtgaggaata actcaccttc gcgggcgtcg agatcctgta gcggaagaac cagcgggccg
    19561 aaaacgaagt caaacagacg cattggctgg gtgattttgc gcatcagcag gcgcccgccc
    19621 ggcttgagca gggggcgcag attatcgagc gtgcggccaa tatggcgggt ggcgtgaatc
    19681 acgttcgctg ccacgataag atcgtaagac tgtgcctgga aaccctgaga ctgcgcctct
    19741 ttttcgagat ccagctcgct atacttcaca aaatcatagt cggcgaattt ctgctgggcg
    19801 cgacgggtga acagcgccga gatatcggtg aaatggtact ccagtgccgg aacgccgttg
    19861 agttccggca gcagccacgc ggtggtgccg ccggttccgc cgccaacttc aagaatacgc
    19921 aacgactggc ggggctgacg cgtctggaca atgccgcgta ataccccggc ggcgatttgg
    19981 ttgaaatagc ggccaaagct gaattcctga tacagcactt ccacgccgtc ggaggcgctt
    20041 tgcgggaaga taatcgccac cggttcttcc gcgccgctca tcatttcata taaccgatcg
    20101 ccggcacggg cgatggtgtc gggaatagcc tgaaaacctt cacaataact ggcaagttcc
    20161 gtcagcagtg attcccgctg ttgatgttca atggggcggg cgcggacgta tcgcccgtcg
    20221 gtgcagcggt aatcgccgtc gaccacgcag ttattcagca ggcgctggag tagctgctgg
    20281 tagcggggca gcagacgtcc acggcgcatg atggtcatgg cgtccacgcc gttctcaatg
    20341 gcatcgccgg tacagcgttg taccagttga tcgacgtaga tggcgtgcag tcgcgtggcg
    20401 cactgtttaa gcgcttccag gcgagggaga tcgagcgctg tcgcggcgcg actcgccacc
    20461 tccagcccgg cagacagcgc tgcgtcggca ggctcgcagg ccggggagac gcgctctttc
    20521 cagtaacgtt cagtatcaaa cggataacat ggcgcagcga tacgttgtcc atcgcccgcc
    20581 agcaggtcgg cccacggtag ggcgacgccg gcagcgtaaa gctggagcag ggcctgattg
    20641 aggacatcgc tcgcctcttt gttacgccgg gcgctggcta tccagtatgc gttatcgcgg
    20701 tattcgcgct gcccgcaagc aaccaactgg gcatcgggcc ccatctccag aaaaacgcgg
    20761 gcgccgagct gatgcgccac ctgaatactc tggataaaac gcaccggctg gcgcatgtgt
    20821 cggcgccagt aatccgcctg gttgagcgtt gactcatcaa tgacgtcggc ggtgagcgtg
    20881 gaaataatcg gtatttgccc cggctccgcg tgcagtcccg cgcaggcgtc ctggaaccga
    20941 tcgagtatcg gctccagtaa agcggagtgc gccgcaccgg ttacgctcag gcgacgatag
    21001 ttaatgtcat gctgcgagag cgtggcgcaa aataccgcga gacgggcttc cggcccggaa
    21061 aataccgtat gttgcgtacc gttgttggcg gcgagatcca gctcaaactg gcgagccagc
    21121 ggcatcagcg tgtcttcgtc tgcaaatacc gccaccatcg cgccgcttgc gcactgctgc
    21181 attagcgcgc cgcgccgaca aaccagtggc atgacctgtt caatcgtata gtgtccgcag
    21241 acaacggcag cggcaaattc accgacggaa tgcccaatgg cgaagtctgg cttcagtcct
    21301 tcagcacgcc agtgcgccgc catcgcgatt tcaaacgcga caatcgccgg ctgcgcccag
    21361 gccatattgt ccagctgcgc cgaatcgggg ttaaacatcg cttcgcgcag tgatggcgtg
    21421 agcatttcgc tacaggcgga aaaacagcga tccagcgtgt cggcaaacgc cgttgagtgc
    21481 tggtacatcg tttggcccat agtgtgccag tgcgagccct ggccggtaaa cagccacacc
    21541 tgcttgccgc tggcgccgtg gccgctgtaa accagcgccc ccgatttctc accggcccag
    21601 gcgctgagcg cctcggcggt ttcacggttt aatggcgccg ccaggcggaa ggggagatcg
    21661 agacggcgcg cgtgcagggc tgtgaaggcc agagagctgg catccgcatt ctctctcagc
    21721 gccccggcat aatccgtcgc cagccgccgc aacgcgctgt cgctggcggc gctgagcagc
    21781 agcgcggtac tttttctgcc gctatccgta ttggggaggc gcgcgttgag cgcgtcgggc
    21841 agcgaggcga cgatcatatg gcagttggtg ccgccaatac caaaggagga gacgcccgca
    21901 tagcgcattt cgtcctgcca tgcctgtgcc gacaccggta tggtaaaggg gctctcttca
    21961 agtttcagcg ccgggttggg ggtgtgaaaa ttcagtaagg gaggaatttg cccgcgactg
    22021 actgccagaa cggttttcag cagtccggca atgcccgccg cggtatccag atggcccatg
    22081 ttacttttca cggaaccgag cgcacagcgc tgatcctgcg ggcgaggcgc atagacgttg
    22141 cgtaacgctt caatttcaat cgcgtcgccc agcggtgtgc cggtgccgtg ggtttcaatg
    22201 taacctacct gcctgtcgtc gatggccgcc agcattaacg cctcttcgat gactgcctgt
    22261 tgccctgcga cggaaggggc ggtatagccg acctttctgt tgccgtcgtt gttgaccgcg
    22321 ctggagagga tcaccgagat aatcggatcg cctgacagca gcgcgtctct caggcgacgc
    22381 agcaccacgc agccgagacc gttaccggcc caggtgccct cagccgaggc gtcaaaggga
    22441 cgacagtgac catcaggaga gaaaatcatt ccgggctggt agcggtagcc tgcctgctgg
    22501 gggaaagaga gcgccacgcc gccggcaacc gccatatcgg attcgccagc gcgcaggctt
    22561 tcacaggcca gatgcacggc aaccagcgag ctggagcagg cggtctgtac cgataacgcc
    22621 gggccgtgca ggttgagttt gtacgcggcg cgggtggcaa tatagtcttt atcattgccc
    22681 atcagagatt gcagaccttt tacctgcgcg acttctgtca cgttcaatgc ttcgcgaccg
    22741 gggtaggtac tcatccggga agaggcgaaa acgccggtct tatgggggac ggcgccgggg
    22801 gcataaccgg catgttccag cgcatgccag accgcctgca aaaacaggcg ctgctgcggg
    22861 tccatcgact ccgcttcctg tcgcgaatag ccaaacaggg tggcgtcgaa gcagtcggcg
    22921 ttgtctaaca ccgaaccgat attgacataa tgaggatcgt caatgatggc ggcatccaga
    22981 ccgacggcca gaagctcttc gcgcgtaaag cgtcggctgc attcacgacc ttccagcaga
    23041 ttttgccaga acgtttcgcc atccggcgat tcaggaaaat ggcaggcgta gccgataacc
    23101 gcgacaggtt cgcagtccgg gtagtgttga gcgatcgatg catcaatgga atctgctgtc
    23161 ggcgcagaag agaagcgcaa gttatccata atcggtcccg tttcctaaga ttcacaaagt
    23221 caatggaacg ccatgcgttc gcgatggcgt tccggggaaa atcagtttgc ttcgcgctat
    23281 atccgccgct gacgacggcg aacagggcga ggtcgttgtg ttttctccac cgtggcgacg
    23341 ccggccagat aatcggccag ggcagcagga gaagggtggg tgaagaggtc aagcaacgtc
    23401 agggtggaaa attcgtgacg ttgtaacaaa acgtgcagtt gcaccagatt cagcgacgtt
    23461 gcgcccgcct caaagaagtt ttctgcgggc gtgacagact cgccaaccac ctcgcggaag
    23521 gcgtcgcaaa tcaggctgac ggtgctctcg tccgccgcaa tgtgtggctg agagtgctca
    23581 gggcatacgc gcggtgagcg ctctgcgcat tgccctgcgt gtttaacggg aggcaccagc
    23641 gcggcgagcg gcagttgcca gccgctttca tcctcggcca ttcggtttag caatgcgcaa
    23701 tactgttcaa actggcgctc cacctgcccg gcaggaaaca gcgcggcgac aaaatcccag
    23761 ttaaagcgca gttcgccttc ggattcataa atctggtgat cgagccagac ctgcggcgtc
    23821 tgggagatgc cccagaccgg tttgagcaga ttacgccggg cgaggaagtt atcctgctca
    23881 aagcccagcg cgctggtaaa gacgacgggc atcggaacgg ctggcacgtt ttgccgttgc
    23941 gccagttgac gcatcacgcg gatggctgac acatcgcggt ggttgaggtt ctggctcagc
    24001 cgctgctgta gtgactgcgc gctgtgcagc cagctttcgc cgggatgcca gctcagcagc
    24061 atcagcgagg tgaaatcgcc cagaatctgg ttgatttgcg ggtgcagcgg tcgcctgtcg
    24121 aaaagcgtaa ggttaagcgt gaagtcaggc tgtgcactcc atgcagagag aaccgttgac
    24181 cacaccgaca acagtacggc cgacggggtg agatgggcgt cagccgcccg ttttttcagc
    24241 cgatgccagc gcgtgctgtc cagtgcgccg ttcaggcggg cgaagcgcgg tgtttcaacc
    24301 tcctgaggca agcagcgcag cggcaacgca ggcgccggag gaatatcatc aagctgcgcc
    24361 tgccaccatg ccagagaatc tggattgggc gactgtagcg agggttgttg cagataatcc
    24421 ctgaaggtga cgggcagcgg cggaagcagc tgttgcgggt agcggtagcc gtgctccagc
    24481 tccgccagca ggatctgcat gctcagaccg tcaagcaaca ggttatccag acacagccac
    24541 aggcgggcag gcatcccgtc cacgtatccg acctggagat cgaataccgg ccatacttcg
    24601 gggttgagta cctgatgcgc cagtttttcg cgcacccgca acgcctcttc aggcgtatgg
    24661 agggtgtgtg cgggtatcac ccaggggggc gtctgttcga gcacctgttg ctgtccatca
    24721 cgcacgatgg cgcgcagcat atcgtggcgg gcgattaatc ggttccagac cgtctccagc
    24781 cgggtgaggt ccagatcggc aatttcaaat tcaacaaaga aatgtgagcc gacgccgccc
    24841 agggcaaagc ccggctgacg ccccaccagg taagcctgct gcacgtcggt aagcgcaaag
    24901 ggctggtagc gatcttcagg ggagtggacg aatggttgtt cgaccgggac gtcggttttc
    24961 cgcagcgtgg cggcaaaatc cgccagccgg ggatgattaa acaggtcgct taattgcgct
    25021 tcataacctg cctgatgaag ttgcccggtc agacgggtcg ccagcaggct atcgccgcct
    25081 tgctggaaga agtcggtttc tctggtgaca ttgccagttg ataagagttg ctgccagagg
    25141 gcggcaacct gtttttcaat gtcgccctgg ggtaaatccg cttcggccgg gttttccgct
    25201 tcaggggtat gacgacgctt cagcgcctgg tagtcaattt tcccgttagc tgtcagcggt
    25261 aaggcgtcga ggaagacgat ccgttgcggc accatccacc ccggcagacg cccggcgagg
    25321 gcggcctgaa gttgacgggg atcgcggcgc acctgcctgt caggacattg tacgaggaag
    25381 cgttgtaacc ccgcgacctc atcgccatgc tgacagttga agccggccgc gctaaataat
    25441 gccgcccagt cggcgctgtt atgcagcaag gcctccggct gcccattggt taacaggagc
    25501 gtgctgagta gggcggacgg cgttaactgg cgaaactcca tcacgtagag cagcgcgccg
    25561 ggaacggcaa gctgttgtaa tgtcgcaagg agcccgggat cttccggcag cagacgatgc
    25621 agggcgttat taagccagat aatgtccgcc gagtgagcgt gcgccgccag cgtgtctgca
    25681 ttccagaggg acagacgggc gccaggccag ggggcgagcc tctgccgggc gctcagcagc
    25741 atctcctggc tctgctcaag cccgacatac tcaatctgtc cggcgttgag ctgtgctaac
    25801 agcgattctg cggcgcggcc agtgcgggtt cccacctcaa gcaggcgtac cgggcgctgt
    25861 aagcgctgtg caagctgttg gcaaattgtc gccagttcct gaatatacgg ggcgctggcc
    25921 ggatggttaa aggccaggct ttccggcgac cactgcgcgt cgttcagtaa cgacagttcg
    25981 ttacgggcac cgcgaagaat ttgcgataac gttaaatcat gtccgctgaa gtgcgggtgc
    26041 tcatcctcgc gttcttcacc agcgcagacc tgataacctt cagcagcggg cttcagtcgc
    26101 cgttgtgtca ccagaaatgc taaccagcgt tccaccacgg cctgccagcg cggctggata
    26161 gcgagtgagt tcatcagggg gagaggatcg gcgccagcgg tgtgacccgg cttcagtttt
    26221 agcaggcgat gctgaaggaa atcggctacc tgttctgcgg agatctctgg cgagatggcg
    26281 caacagggca acgttcccgc aagcgtgtgc cacgcctgcg gcagtgccgg gttccgatga
    26341 tcggtaacgc aaaaagcctc gccctgagga acaacgtatg ccgccagcgt tttttctttt
    26401 tcgccgatcg ccagaacggt tgcttgtttc acccccacca gctggctgag cgcgctttcg
    26461 atttcgccca gctcgatgcg atatcctccg actttgacct gcttgtcgcg acgaccgagg
    26521 aactcgattg tgccatctgg ccagtagcag ccgagatcgc cggtgcgata ccagcgctcg
    26581 tccgggagcg tcaaaaattg ctgctcgcta cgcaggggat cgttgaaata gccttccgcg
    26641 accccaatgc cgccaatcca taattcaccc ggcacccagt cagggcagtc ccggccctgt
    26701 tcatccacca cccggtagcg ttggttggtt agcggaaaac cgtaagggat ggagcgccag
    26761 taggcgggga cgtcgtgaat ttcgcaggcg ttagaccaga tagacgcctc ggtggcgccg
    26821 cccatcgcga taaattgtcc ttgtggccgg aaggcccgat aacgggcggg gaggtcaagc
    26881 ccgatccagt cgccggaaag catcactgcg cgcaggtttt ccggcgtggc gtcggcgaaa
    26941 ccttcacacc aggtcagcag catatcgaac agcgccggga cgctgttcca gagcgtgacc
    27001 tgatggcgct ggatcagctc acaccatgcg tgaggatcgc gccgttgatt ttccatcacc
    27061 atcaccagcg cgccgcccgc gcgcagtacg ccaaaaatgt cgtaaaccga taaatcaaaa
    27121 tgtagggcgg agagggccag caccctgtca tgcgggccaa cctgatagcg ggtattgata
    27181 tcgcaacagg tgttaagcgc tccccggtga gaaatgacta cccctttcgg cgtaccggta
    27241 gagccggagg tgtagataat gtaggccggt tgcgtggggg cgcgtactac cgggttggcg
    27301 atcggctccg cctcaatggc ctgctgccag gcaagggcgg gaatatcgtc tgacccggcg
    27361 ctggcgtcgt gctgacaaat gagcaccagc cggacgctgg cgtcagcgta gattttctcg
    27421 cgccgtgcgg caggctgatc cagcgaaacc ggaacgtaaa ccgccccggc cagcaggacg
    27481 gccagaaccg caacaagttg tcctgcgcct ttcgacatcg tgatagccac attatcgccg
    27541 ggctgaaccc cgcactcgat taacctgccc gcgcaacggc gggcatagtc tgtcagctca
    27601 tgataattcc actgataacg catgtccgtt accgccagcg cctgcggctg ttgcagagcg
    27661 atacggaaaa tgccttcatg cagcaagcct tcgggaatgg gggcgccggt ggcgttgacc
    27721 cgttcgcgta tcgcgcgctg gctggcgggc atcatatctg cgaacggctt ttgccaggcg
    27781 ctttcgtcat cgcagagttg gttaatcaac tggcagtagg cgtcgaacaa tgtttcgact
    27841 aacgccggag ggaacagcgc gtcgttgctg tcccattgta gccagacctc gccgtgatgc
    27901 tcgaacgcca gatgatctat ccagacctgc ggcgtttgcg agatgcccca ttccggctcg
    27961 cccaacggcg attctgcgcg gctgctgtag agggaacgcc ccagattgct ggtaaatacc
    28021 accggggcgc cgtgggggta gcgctgctgg cgtttgagtt cacggagtaa ttcgacgccg
    28081 gaccagtggc gatgctccca gtcctccgta aacgtgagct ggtttttacg cgccaggttg
    28141 ctgacggtat cgccatcgca ggcggtatcc agcagaagaa tattggtgaa gtcggcaagc
    28201 atcgcgccaa ccgccggttg cagcggctgg cggtcgaata aggtgatgtt aagcagcaga
    28261 cgcgtcaggc cgccccagcg agccagcacg gcagaaaaac aggtcgccag tgccattgtc
    28321 ggcgtcacgc catactcgcc ggcccggttg ctaaaggcgt gccagcgtgt tgccgggaca
    28381 atcatgcggc gtcgggtatt acggacttca cgtagcgtgg cgggttcgca ggccagcggc
    28441 aagacgggcg cggggggaag cgtcgatgct ttcgccagcc agtaagcgcg cgcgacgtct
    28501 ctcagtggtt gattgatctt ctgctggtgc agcaaatacg agcggaaatc atagcgggtg
    28561 tcgatagccg gcagcgattc tccggccagc agggcgttaa gctcatcgaa gaaaagcgta
    28621 aagctggagg catccataat cagcaggtca atattgacat ggaggcggtg gcgattgtcc
    28681 ggcaagagcg tcagctgaaa atcaaacgtt tcgccgattt ccacgcgtaa aagacggtgg
    28741 ctcaggcgct ggcgcagtgc gtccagatag gcctggcggc tttcagcgtc gttatggcgt
    28801 aaatcatgaa cggtgacgcc gttccagtaa ggttgcggta gccagacctg ctgcccgtcg
    28861 gggcgaaagg cgatatgcag cattgggtgg cgttgcagca aggtcgtgat ggcctgctcc
    28921 agctgcgacg ccgtcagaca atggccttca aactcctgat acaggtggca acccacgccg
    28981 ccaagcgtct gccccggcat gcggcccgtc aggtaggcgt gctgtactgg cgtcaatggg
    29041 aagggggtac tttcggtcat gttcggccag gatgattcgt cgggcggcgt ttcttcttcc
    29101 gcgttctccg gcgaccggct gagcattaac tggttccatg ccgccagcgt gggggcggca
    29161 tacagctcgc gaagggtaag gcggtagcca tttttacgaa accagtgtaa ccatctcatc
    29221 aatcttatgg aatccagacc ggcctggatc aggttgctct cttcatgtaa ctgctgcggc
    29281 gttaaattca gttcctgtat gagccgctcg cgtaattgtt ggtaatcagc cgcgtggcgg
    29341 ttgtccggta acagcgaatc ctgagatggt gcgccagaaa tcattcttcc tcctgatggc
    29401 acgtcttacc gaaaagcatt actcatatga gaataataat tattaacaat tggatgctac
    29461 ccgaaacggg tttatttatg cccgtttcgg gttggtaaca ggcggaaaga acagaggccc
    29521 ggatggggta tccgggcctc tgtcagggag gagtttaggg gggcgcgacc ccggttacat
    29581 cccgcgttta aaggtcgaag gagttacgcc aaactgtttc tggaaggcgg cagagaaatg
    29641 gctggcgttg gcatagccca gatcggccgc caccgtcatg acggaggtat tgccgttggc
    29701 caggcgtctt ctggcttcct gcattcgttc agcctgaaac aggccataga cgctgttgtt
    29761 gaacagaacg cggaagcctc gcttaatttt gagaacgctc agaccggact ctctggccag
    29821 ctccgcgacg cccggcgctt gcgtcagatc agccagcagc agatccttcg cccgcagcag
    29881 tttctggcgt tccatcgggt tgaagtggca ggagaggcaa ctggcgtctt cgcgctgttc
    29941 atccagcacc agactgagca tgaccagact ttgcccctgc aaccatagcg tcgacggctt
    30001 ttgttcatga ttgaagccgt cgtgcatgcg catcagcgcg tgacgcaagg cctgagccgt
    30061 taagtgggtt tcagcattac aacgctgctg ctggcaacag cagtgggagt cgatcttatt
    30121 cttaatgacc gcgctaatat ccggcaccca cagcgcgagg gtttcagggc tgaaggagag
    30181 ggtaatgctc tcgaatcggc cacaatagga ggcgctgccg cgacagtctg gcgtatgggt
    30241 aatgtaatgg cgattgcgcg caagctccca gtcggtgtgg cgccggagtc cctgaatcgc
    30301 aaagcgcgat gtgccttcca ggatgcacga gaagttaatc ctgcccagat cgtcgtgtag
    30361 ctgcaacgtc tcaggccgcg caaaatggcc cctccagagc aaaatatcca taccttcctg
    30421 caacgaacag cgcagcagtt cacactgagc aggaatattg ccatcgtttg caggtttccc
    30481 gaccaccaac tggtgaatag agatttcaga ttgcgtttgc ggtgactccg tcatgacctg
    30541 gttatctccc tgtggcttag accaatgcgg gtataaatag aaccgaatgg ggtagatgag
    30601 ttattgataa tggttattat tcacattaat tctcagaacg ccaccctgaa ttgtcgtttt
    30661 tggcgtccgt aaaaatgcat caggaaattc agttactccc atgtcatctc aatcatccaa
    30721 tactgaatcg ctgtcccgat ttcctctctg gcaggtgatt acaccggttc gcaggaaggt
    30781 tattttagct atggcgctgg cggggctggc ggcattaacc agcctggggg cgctcctttt
    30841 tctggtgtgg agcctgcgtg acattcgcgc gacgcctgac gctattccgg cctggccgct
    30901 gggtggcgtg atcggctgcg tggtcttaac ttttgttctg cgcttacagg cgttcaacac
    30961 ctctcattac gcggcttttc atctggagaa cattctgcgc agcaggttag cccgtaaagc
    31021 attgcagctt ccgcctggcg tgttacagca aatgggcagc gggtcagtgg cgaaagtgat
    31081 gctggatgac gtgaagtcgt tacatatttt tgtggccgac agcacgccgc tctatgcccg
    31141 cgcgatcatc atgccgctgg cgacaatcgt tatcttgttc tggctggact ggcggctggc
    31201 aatcgcgacg ctgggggtac tggcgtttgg atcggttgtt ctcgtgctcg cccgccagcg
    31261 ttcggaagat atggctcagc gttatcataa ggcgcgcgag caggttagcg cggcggttat
    31321 tgagttcgtg caggccatgc ccgtggtgag aacgtttgat agcggcagca ccagtttttt
    31381 gcgctatcaa cgcgcccttg aagagtgggt cgatgtgctc caaacctggt atcgcaaagc
    31441 cggtttttca gcgcgttttt ccttctcgat tctgaatcct ctcccgaccc tgtttgtcct
    31501 gatctggtcg ggatacggcc tgttgcacta tggcagtttc gattttatcg cgtgggtggc
    31561 cgttttactg ctgggcagcg gaatggccga agccgtaatg ccaatgatga tgctcaataa
    31621 cctggtcgcg caaacgcgtt taagcattca gcgtatttat caggttctcg cgatgccgga
    31681 gttatcgctg ccgcagtctg agcagcagcc gcaagaggcg agcattacct ttgagcaggt
    31741 gagctttcat tatccgcaag cgcgtactgg cgccgcgttg caggaggtga gctttcatgt
    31801 gcctgccggg caaattgtgg cgctggtcgg gccaagcggc gccggaaaaa gcaccgtggc
    31861 gcgcttgctg ctgcgttacg ccgacccgga caaaggccat atccgtattg gcggcgtgga
    31921 tctgcgtgat atgcagacgg acaccctgat gaagcaactc tcgtttgtgt ttcaggacaa
    31981 cttccttttt gtcgacacga tagccaataa cattcgtctg ggcgcgccgg atacgccgct
    32041 ggaggcggta atagcggcgg ccagagtggc gcaggcccat gattttatta gcgctctgcc
    32101 agaaggttac aacactcgag tcggggaacg tggggtattt ctctccggcg gccagcggca
    32161 gcgcattact atcgcccggg cgcttttgca ggatcgcccc atcctggtgc tcgatgaggc
    32221 gacggcgttt gctgacccgg aaaacgaagc ggcgcttatc aaggcgctcg cggcggccat
    32281 gcgtggccgg acggtcatca tggtcgcgca tcgtctctca atggtgactc aggccgatgt
    32341 gatattgctg ttttccgacg gacagctcag ggaaatgggg aaccatacgc aactgttggc
    32401 gcagggcggc ctgtatcaac ggctctggca acactatcag caggcgcagc attgggtgcc
    32461 gggtggaaca caggaagagg tggtggaaaa tgaaagacaa taatcctgcg gataacctgg
    32521 cctggcgcgt catctggcgc cagcttatct ccagcgttgg cagtcaggcc aggatgctgc
    32581 gacgcagtat gctggcgctg ttgctggcgg cattcatgca ggggatcgcc tttgcctgtc
    32641 tttatccgat cattgatgcg ctgttacggg gagacgcgcc gcaacttctt aactgggcta
    32701 tggccttcag cgtcgccgca attgtgacgc tggtgctacg ctggtatggc ctgggctttg
    32761 aataccgtgg tcatctggcg caggctaccc atgagttgcg cctgcgactt ggcgagcagt
    32821 tacgccgcgt gccgctggag aagctccagc gcggcagggc gggtgaaatg aacgccttgc
    32881 tgctgggcag cgtggatgaa aacctcaatt atgttattgc gatagccaat attttgctgc
    32941 tcaccattgt cacgccgctg acggcgtcgc tggcgacatt gtggatagac tggcggctgg
    33001 ggctggtgat gttgctgatc ttccctctgc tggtgccgtt ttattactgg cgccgcccgg
    33061 cgatgcggcg acaaatgcag acgctggggg aagcgcacca gcgtctgagc ggcgatatcg
    33121 ttgaatttgc tcaggggatg atggtattgc gcacctgcgg cagcgatgcc gataaaagcc
    33181 gggcgctgct ggcgcatttc aatgcgctgg aaaacttaca gacccgcact caccgtcagg
    33241 gcgctggcgc gacgatgctg atcgccagcg tcgtggagtt gggcctacag gtggtggtgt
    33301 tatccgggat cgtctgggta gtgacgggca ctctgaacct cgcctttttg attgccgccg
    33361 tcgcgatgat tatgcgcttc gcagaaccga tggcgatgtt tatcagctac acctcggttg
    33421 tggaactgat cgccagcgcc ctgcaacgga ttgagcagtt tatggcgata gcaccgcttc
    33481 ccgtcgcaga gcaaagcgag atgccggaac gttacgatat ccgctttgac aacgtcagct
    33541 atcgctacga agaaggcgac ggccacgcgc ttaatcatgt ttctttgacg ttcccggcag
    33601 ccagtatgag cgcgctggtg ggtgcctccg gcgcaggcaa aactaccgtc accaaactgt
    33661 taatgcgcta tgccgatccg cagcaagggc agatttctat tggcggcgtc gatattcgcc
    33721 gcctgacgcc ggaacagctc aatagcctga tttctgttgt tttccaggat gtctggctgt
    33781 ttgatgacac gctgctggcg aatatccgta tcgcgtgccc acaggcgacg cggcaggagg
    33841 tagaagaggc cgcccgcgcg gcgcagtgcc ttgagtttat ttcccgtctt ccgcaaggct
    33901 ggctgacgcc aatgggtgag atgggcggcc agctatcggg cggcgagcgc cagcggattt
    33961 ccattgccag agcgttattg aaaaacgcgc cagtcgtcat tctcgatgaa ccgactgccg
    34021 cgctggatat tgaaagcgag ctggcggtgc aaaaagcgat cgataacctg gtttacaacc
    34081 ggacggtgat tatcatcgct caccgtttat ccaccatcgc cggggccgga aacattctgg
    34141 tgatggaaga gggacaggtg gttgagcagg gtactcatgc gcaattgctc tcacatcatg
    34201 ggcgttatca ggcgctgtgg caggcgcaaa tggccgcgcg cgtgtggcgc gacgacgggg
    34261 tttccgcgtc tggagagtgg gtgcatgagt gatgttcagt cgaatgtgaa accgctgacg
    34321 ttgacgaccg ggcgggtgat ttttgctatt gccggcgtct atgtgacgca gagtctggta
    34381 tcggcgctgt ctatgcagtc cttacccgcg ctggtgcgcg ctgctggcgg ttcgctggcg
    34441 cttgccggtg cgacaaccct gtttatgctg ccctgggcgc tgaagtttat ttgggcgccg
    34501 tggatcgagc gctggcggct tccgcccggt agccaggaac gccgttcccg catgttaatc
    34561 ctgcgtggtc aggtcgcgct agcggcgatc ctgactattg ccgcagcgat tggctggttt
    34621 aggcgagaag ggggattccc cgatacgcaa atcgtcgcgt tatttgttct gtttatggtg
    34681 gcaggcacgg tcgcctccac cattgatatc gccagcgacg gcttttgtgt cgatcaactg
    34741 acccgcacgg gttacggctg gggaaacagc gtgcaggtcg gcggcagcta tctgggaatg
    34801 atgtgcggcg gcggggtgtt catgatgctg tcggcggcat ccggctggcc tgtcgccatg
    34861 ctgatgatgg cggtgctgat tatggcgctg tcactcccgc tgtggcgcat tacggagccg
    34921 acgcgaacag cgactatcct gcatgttccg gcgttaggtt atgcgctaag gaggaagcag
    34981 gcgcgcctgg gcttactgct ggtattgatg ctgaattcag gcatgcggtt tgtgctgcct
    35041 cttctggcac cgctgttgtt ggatcatggg ttgagcatgt ccgcattggg cgcgttgttc
    35101 agcggcggca atattgcagc gggcatagca ggaacgctgg ctggcggatt gctgatgaaa
    35161 tacacctcac ccggcagagc gctgttgacg gcttatggcg tccaggggat cgcgctgctg
    35221 gcggtggtga tgacgctcat gatggcgccg ggtcatctgc tgctgcagat tctccagtgt
    35281 ctggtcattg tccagtccat ttcgctggcc tgcgcgctgg tctgtcttta cgccacgctg
    35341 atgtcgcttt catcgccttt gcaggccggt gtcgacttca ccctctttca atgtactgat
    35401 gcggcaatcg ccattctggc aggcgttatc ggtggcgttg ttgctcaaca ttttggctat
    35461 gcggcctgct tcctgtttgc cggggcattc acgttgctgg cggcgtgggt tgcttatatc
    35521 cggctgcatt cggcaagaga actgatgaca agcgcaattg attgatccgc gaataacaca
    35581 gagaggtaag ggatgaaaat cagtgaattt ctacatctgg cgttaccaga ggaacaatgg
    35641 ctaccgacga tttctggcgt tttacgccag ttcgcagaag aagagtgtta tgtctatgag
    35701 cgtcaaccct gttggtattt aggcaaaggg tgccaggcac ggctgcacat taatgccgac
    35761 ggaacgcagg cgacatttat tgatgatgcc ggggagcaaa aatgggcggt ggattccatt
    35821 gccgactgcg cgcgtcgttt tatggcgcat cctcaggtga aaggacgtcg ggtatatgga
    35881 caggttgggt tcaactttgc ggcgcatgcg cgggggattg cctttaacgc cggggagtgg
    35941 ccgctgctga cgttaaccgt tccccgtgaa gaacttattt ttgaaaaggg aaatgtcacc
    36001 gtttatgcgg actccgccga cgggtgccga cgtttgtgcg agtgggtaaa agaggccggt
    36061 acaacgacgc agaacgcacc actggcggtg gataccgccc tcaatggtga ggcatataaa
    36121 caacaggttg cgcgcgccgt tgcggagatc cgccgtggcg agtatgtcaa agtgattgtc
    36181 tcgcgcgcca ttcccctgcc atcgcggatt gatatgcccg ccacgctgtt atacgggcgg
    36241 caggcaaaca cgcctgtgcg ctcgtttatg ttccgtcagg aaggacgcga agcgctgggc
    36301 tttagcccgg aactggtgat gtcagtgatg ggcaataaag tggtcactga accgcttgcg
    36361 ggcacccgcg atcgcatggg aaacccggag cataataagg cgaaagaggc agaactgctg
    36421 cacgacagta aagaggtgct tgagcatatc ctttctgtca aagaagctat tgctgaactg
    36481 gaggctgttt gccagccggg cagcgtggtg gttgaagatt taatgtcggt tcgccagcgc
    36541 ggcagcgttc agcatctggg gtctggcgtg agcggtcagc tttcggaaaa caaggatgcc
    36601 tgggatgcgt ttaccgtgct gtttccgtcg attaccgcct caggtatccc taaaaatgct
    36661 gctctgaacg cgattatgca aattgagaag acgccgcgag agctttattc cggcgcaatc
    36721 ctgctgctgg acgatacgcg ctttgatgcg gcgctagttc tgcgttccgt atttcaggat
    36781 agccagcgct gctggataca ggcgggggcg ggaatcatcg cgcaatctac gccggaacgc
    36841 gaactgacag aaacccggga gaaattagcg agcattgcgc cctatttaat ggtgtaggag
    36901 taactgtttt ctaacctcac aaccagactg tccaaagaca attgagtcgc tctttgattc
    36961 ctctgtgact ctattgtctt cttttgggat aaaataggtg tttttcgtca tgaagttgat
    37021 aaaagtctat atagaaccac ctcctgtgga ggtggtggta atttgccaga gtgagggatt
    37081 cgttagtata ctatgggata aacttatttt taccaattgt ataaggcggc gcataagatt
    37141 ctcggcatgc ttccagataa tccgaccacc actgcatcat cgctttgcgg gcttctaggt
    37201 gctctgcctt atgaatataa gccatgcgca cggtattgcg ctcctgatga ctcatctggc
    37261 gttctactgc gtcctttgcc cataaacccg attccatcag cgcactgcat gccattgccc
    37321 ggaagccgtg accgcagata tcctttttcg tgtcgtaacc catcacccgc agtgccttat
    37381 tgaccgtgtt ttcacacatt ggcttatacg ggttatggtc gccggggaag atcagttcat
    37441 tattaccggt gatatcctta atctgtttca gaatggcgat ggactgttct gagaggggga
    37501 cgatatgcgg cattcgcatt tttgccccgc ggccggaata acgcacgcca ataatgggtt
    37561 ctcgcgtcgc gggtatcgtc cagactcggt ttgtgaaatc aatctctgac cagcgggcga
    37621 aacgcagttc actggagcga atgaacacat gcagcatcag cagaacggca tgccgggtca
    37681 gttcacggcc ctgatgatat gccccaatac gttcaagcag ttcaggcagc cgctccagcg
    37741 gcagggcagg atagtgccgt ctgacaggag gtgtggtcac gccgccaagg tttgctgcag
    37801 ggttcgtatc gattaactcc tgatggaccg catggcgcat tatgttactc aggtgctgcc
    37861 gtgtgcggga cgcaacctcc agctttgaga taccagtggc gggagccacc cggcttgacc
    37921 agcagataca gaccgtgaga atcggagact ttaaagggtt tatcagaagg cttgagggtg
    37981 cggatttttg cgtcggtaag gaacatatgg gggtcactcc attatcgaac taacctgatc
    38041 ccagatttga ccaccaattt ttcccgatgc atagggtaaa atcaaaatgc accgagaaga
    38101 gttttcacac taacctgttg aatcaacatc agataaagat ttgcaaggat gcatgaaaac
    38161 agaaaattgg ctcctctgac tggactcgaa ccagtgacat acggattaac agtccgccgt
    38221 tctaccgact gaactacaga ggaatcgtgt gaacggagcg catattactt agcggtacct
    38281 tgtctgtcaa cactaaattt cataagccat ttcaattggt taattaatct gcaaagttgt
    38341 taattaatga acattcgtcg ccgaaaacga gtctgtatca ttagcgtgat gcagtctctg
    38401 caaaggatct tgttgataaa attgacagaa acgttgccac aatgaaggga aacgaggagc
    38461 aaaaagttct ggggcgctaa agaaatattc agaaagtacg gcaaaacatt cagcaggatc
    38521 actggcagca taagcatcaa tgctcgctgc attctcacca actaattcga tttcttcctg
    38581 aatgttgttc attgcagcat gaagatcgtg ttcccagcca gcaacctcac gcaacgaaat
    38641 aaagggaact ccgctggcgc gatcgccgtt acgggtgtcc agcttatgag cgacttcatg
    38701 aataatcagg ttaaaaccag atgcatcaaa agaatcttgt atatccaacc agttcaaaac
    38761 gataggccct tgctgccagc tctgacctga ctgaacaata cgttggttat gcaccagacc
    38821 gatatcgtct tcccattcat catcgaccac aaatggcgca ggataaatta agacttcatg
    38881 aaaaccatcc agccattcca gtcctaactc cagaacgggt aggcaaaata gaagtgctat
    38941 ccggcagctt cttaatgaat ccagttcaaa gccctgtaaa ggaacaagcc gcttttgctg
    39001 taaaaaacgt tcggcaagag tgactaattt gctttgttcc tgttccgtca gacccgttaa
    39061 aagggggatc gatagtgctt cctgccaggg aagggcagtt tgatgtgctg attcttgtac
    39121 tttccagggc cacttaatca tcgttttgct cgcaaactcg tcacttgaac aaaattgcac
    39181 ggacagggac tgttaaaatg ccaaatttcc tggcatcatg gcaaccatct gaacggagag
    39241 atgccggagc ggctgaacgg accggtctcg aaaaccggag taggggcaac tctaccgggg
    39301 gttcaaatcc ccctctctcc gccactttat caatgactta tctcctgact tcccgccttg
    39361 cttttcctaa acagaacaat cgtagaatat tcttgaaggg ttagatcgtc actgttttct
    39421 gttcgatact gtgacattca gcacttgatt cgctatggag ctgacaggaa ggtttcgagc
    39481 gaaaatctac agttattcag tcgttttctt atcggtcacc attattcttt tagacattga
    39541 tcctatgaaa ctgccgcaaa gttgctggcg ggaactgaag ttgcgtagag aaggggtcaa
    39601 taattgagtc aaaaaaggaa cgcggccgtc agattaaagc cgcgggacaa agtccataat
    39661 ttccaagagt tgttgaattt cccagctttt ttgaaagcct ttgtatcata ctaacaagta
    39721 aaaaaggttt aaacaccaaa attaacctat attactggtt agctttacca tgacagatga
    39781 cttgctcctg gaaacatttg cgctgccagt ctgtatggct gagttgtgta tttattgttc
    39841 gcctttttcg catttttttc tacagacata ataaaaaaga cacgactcaa gtatgagcgt
    39901 gtctgagttt gtcttactta tttgtcttgg caaattattg ttgttactac caggcactgt
    39961 tctgttaaat aactgaagtc agcgcaaaaa aatcaatgag acatcatctc ctcgaccact
    40021 tcttcgctac tcaactgata gggataatac gttggccagt tttccatttc attcaataaa
    40081 gactgttggg aatcattacc cataaaaatg tgaaaatgag atgattttcg tggtgcaatt
    40141 atatgatcgc taaattgtat atatttaggg gctttacttt caggatcttt acattcgaat
    40201 aagtaacgaa cgcctttctt gcctgattta taggtgagta ttttgtatcc atcgtaatca
    40261 tatttacagg atgttgtttc attatttcta tggaattcga caatgccgtc ctcaatgcca
    40321 atcatctcga tgtctgtcac ataacctttg cgataataat ctttaatttc agcaaatgtt
    40381 ttggttttat ctgcatccgc tttcttctga aagatggggt caagtttgcc actttgcagt
    40441 aaaggataaa cggattgcca gactccatcc cagtcactga gcgttcggtt ttgtacattg
    40501 gcatcatcaa aaacaccatt agcagctttt tgttcgacct ctgttaaggg tttgccgtgt
    40561 gagtgatgac catgcgaaaa ggcaggagcg ctaacaataa agacacctaa agcaacagcc
    40621 agtttatgaa gacgaatcgc caaaacagtt tcctccagag ttaatgtata tgatgtgtaa
    40681 tgttatattg taacatataa caaaatgcaa caactcttag gagacaagag aatagttctc
    40741 tcaggtgtgc ttatctatct tattgaaata aaataaatta tgtcgatgat ttgaggttga
    40801 tgacaaacga aacataactt gatgtgatct gcgtaggccg gatagggcgc tcgcgccgca
    40861 tccggcaggg ataacgcgta ctttgtcggc aatatgaagt tatacagata atttgttgtg
    40921 aacctgcttg cgtaaccggt taaaaaggga aagcaacttc ttataccgta aggctaaaag
    40981 cagtaccgcc agcccagcgt agatgagtgg ctgcggtgaa ataatcttca ctgaccacaa
    41041 ataatgtatt ggggccagga tcgcgacaag atagacgaag ttatgcaact gttgccagtg
    41101 tttaccgagt tttcgctgca tcgcctgggt tgaagtgaac gctaacgcaa gcaaaattac
    41161 ccagctgata atacctaacg ttaaataagg ccgggtaatt aactccttac ctaataacgc
    41221 cagattattc acgcccagct ccagcaatgc gtaactggtt aaatgcagtg tcgcccaggc
    41281 aaagcaccat aagcctaaca ggcggcgagt gcgtatcaat aacggctgtt ttgcgtagcg
    41341 tgccagaggt gtgattaaca aggtcgccag caaaaatttc agtgcagtgc gaccagtaaa
    41401 atgctgaata tctttcaccg gatcggcacc caatccaccg tgattaatcg cccagaccag
    41461 ccagagaaac ggcaacaatc cggcaagatg caggcaaact ttcagccatg tcacctgttt
    41521 tgctgtcaga cgcatttaga aattctcccg caaatccagg ccacgataca gcgatgccac
    41581 ctgctcggcg taaccattaa acagtagcgt tggctggcgc tgtacatcga ggatgccgcc
    41641 tgaaccaata aatcgttcgg tagcctgtga ccagcgcggg tgatcaacat gcggattaac
    41701 gttggcgtaa aaaccgtatt cgtcaggcgc tgccagattc caggtggttg gcggacgctc
    41761 gcgggtcagc ttaatactga ctatcgattt aatcccttta aagccatatt tccacggcac
    41821 aatcagtcgc accggcgcgc cattttgtgg cggtaacgcc ttgccataaa cacccacggt
    41881 catcagtgtg agcggatgca ttgcttcgtc gagacgcaat ccttcgacat aaggatattt
    41941 cagcccgcca ccgataaagc ggtcctgctg gcctggcatt tgttccggtg cataaattgt
    42001 ttcgaaagcg acatacttcg cattgctggt gggttcggca agcgccagca atttgtgcag
    42061 cggaaaacca atccacggca ccaccatcga ccatgcttcc acgcagcgca tacgataaat
    42121 acgctcttcc agcggaaagc gacgggttaa atcatcgtga tcgagggtca atggttttgc
    42181 cacttcgccg ctgattttca gtgtccatgg atcggttttc aggctaccag cattagcggc
    42241 gggatcggct ttatccagcc cgaattcata gaagttgtta taaccggaga ctttatctgc
    42301 tggcgtcagt ggcaggtcat tttgccaggc ggcaggcttg ctgaactcca acggttttcc
    42361 ggcgggggcg ggtgggcgat cattcccttt gaaccagcta agcagatcgg catgcgcagc
    42421 gtgaggcaaa gaaagtgcag ccgcgctgat gcccagtgct tttaacacct gccgacgcgt
    42481 cataaagaat accgactcgg ccgtaacatc tgattctttt aaaaattgat tctttttcat
    42541 aagccatcac acttttttgt ctttagccag caagagccgt ttactgttaa tctgtcagga
    42601 agtgtcctcc ctaacagtag cagagatctg cttttacata tttatgcatt aactgccacg
    42661 ataggttgaa tacccatatt ggctaagtaa taaaggcaca tgataatgct cgttcacttt
    42721 attaatatga aattcaacgg ggatctcagg gaagaaactt tcaagatttt gtttcttgaa
    42781 atagtccccg gttttaaata cgacacggta atcgcccgta gttgcagttt gctcgggcca
    42841 cagtgcctta attcgtccat ccttatctgt tttggcggta ttaagttgta accagccgtt
    42901 gtccgctttc ttttcaagag tgactgtcac gtcggcagcg ggttttcctg tttgctggtt
    42961 caaaatgtgc acactaagaa tgttttgttg tgcagcatag accaaagaag gtaatgaaaa
    43021 tgcagccgtt actacggaga gtactaaata acgctttaac atgtttatat ccttgtcatg
    43081 tgaatgagtt ttgaggatag cgaaaaagcg tccacggtaa ccttacagca gcattacaat
    43141 tttgtaatga accaggctgt ttctataaca tatgatttat ggcatattat tttcatgaag
    43201 attttactta ttgaagataa tcaaaggacc caggagtggg taacgcaggg gctttccgaa
    43261 gcgggttatg tcattgatgc cgtttctgat ggcagagatg ggctttatct tgcgcttaag
    43321 gatgattatg cattgatcat tctggatatt atgcttccgg gtatggatgg ctggcagatc
    43381 ttacaaacgt taagaacagc aaagcaaact cctgttattt gccttactgc aagagattct
    43441 gtcgatgaca gagtcagagg gctggacagt ggggcaaatg attatctggt aaaacctttt
    43501 tcattttctg agttgctggc aagggttcgg gcacaattaa ggcaacatca cgctttgaat
    43561 tcaacattag aaatcagtgg cttaagaatg gactctgtta gtcaaagtgt gagcagggac
    43621 aatatcagta ttacactgac gcgcaaggag tttcagttac tttggctact ggcctccaga
    43681 gctggcgaga ttatccccag aacggttatt gcgagtgaaa tttggggaat caactttgat
    43741 agtgatacca atacggtgga cgtcgccatt cgcaggctcc gcgcaaaagt tgatgatcct
    43801 tttcctgaaa agctaatcgc cacaatccgg gggatgggct attcattcgt agcggtaaaa
    43861 aaataatgaa aagattatct ataaccgtcc gtttaacctt gctttttata ttgctgctat
    43921 ctgttgctgg cgctggaatt gtatggactc tctataatgg cctggcaagt gagttgaaat
    43981 ggcgcgatga tacaacactc attaaccgga cagcgcagat caagcagttg ttaattgatg
    44041 gggtaaatcc agatacgtta cctgtgtact ttaaccggat gatggatgtt agtcaggata
    44101 tcttgatcat tcatggtgat agcatcaaca aaattgttaa ccggacgaat gtcagtgatg
    44161 acatgttaaa taacatacct gctactgaga caatcagcgc agctggcatt tacagaagca
    44221 ttattaatga tacagagata gatgctttac gaattaatat tgatgaagtt tcgccatcat
    44281 taacggttac tgtggctaaa ttggcttcag ccagacataa catgcttgaa cagtataaaa
    44341 ttaatagcat tataatttgc attgtcgcca ttgtactttg ctcagtatta agtccgctgt
    44401 taatcagaac gggattacga gagatcaaaa agttgagtgg tgtaacggaa gcgctgaatt
    44461 ataacgatag ccgggagcct gttgaggtta gcgcattacc gagagaacta aaacctcttg
    44521 ggcaggcgtt gaataaaatg catcatgctt tagtcaaaga ttttgagcgt ctaagtcagt
    44581 ttgctgacga tctcgctcat gaacttagaa cgcccattaa tgcattactg ggtcagaatc
    44641 aggttacgct cagtcaaacc agaagtatcg ctgaatatca aaaaacaatt gccggtaaca
    44701 ttgaagagct ggaaaatatt tcgcggttaa cagagaacat actgtttctt gcccgggcag
    44761 ataaaaacaa tgttttggtg aaactggact cactttttct caataaggaa gtcgaaaatt
    44821 tgttggatta tcttgaatac ctttcagacg agaaagagat ttgctttaag gtcgagtgca
    44881 atcagcaaat ctttgcggat aaaattttac tgcaacgaat gttatcgaat cttattgtta
    44941 atgccattag atattcacca gaaaaatcgc gcattcatat aaccagtttt cttgatacca
    45001 acggctatct taatattgat atcgccagtc ctggaacgaa aattaatgag cctgaaaaac
    45061 tcttccgtag attttggcgg ggagataatt cgcgtcattc cgaaggtcag ggacttggtc
    45121 tttctttagt caaagcgatt gccgaattgc atgggggaag tgctacgtat cactatttta
    45181 ataagcataa tgtgttccga attacgttac cgcaaagaaa ttaacatagg gcttcagtac
    45241 gccataaatc aaccatgaaa aaacgcagcc acgtcgtgag tattaacgtg gctgcgattg
    45301 attatgcgct tacattcata cgtaacaggg attaacccgc ataagctgcc agcatttcct
    45361 gcgccgccag tttacccaac gcattcgctg cgaaaggagt gtcgccggtg agaactttac
    45421 ggtccttatg tactcgcccg gtgatgtcgt cattaataat gttcatgccc attttcttca
    45481 gctcttcgcc gaagtaccag gtgagatgac ccggcatata gcctatctct ggcgtttgtt
    45541 tgtctgcggc gtctgggaat gcgcaaatgg aataaccatt cagtgggtta tcgccgtggc
    45601 gaagcgccag aaaagccgcc gggccgtggc aaagggagat aacaaaacgg tcatttttga
    45661 ttgcccactg caaagcagca gccacgtcct ggctttctgg taaaccaata agtgcgccat
    45721 gaccaccagg aacaaagatt gctgcatatt cgctgtctgc gttgaggctg acgacaacat
    45781 ccgcgatttt tttcggattg cggaacaacg atttatgctg ctcaaagaat ggcattactt
    45841 tttcatcttt gtgcggcata gcccagtatt caaacttggt catcagaccg gaaatggtcg
    45901 ccacttcgaa ttcgaaacct gcagcatgga gatgatacaa cggcagcaac gtttcaatcg
    45961 gatggttacc ggtcgagaac agttttccgt tatcggtcgg caaataacgt tcgtccgcgg
    46021 cgatcaccag aattttatgt ttaccgcgat acggttttg
//
LOCUS       DAZGOB010000024        45724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGOB010000024 DAZGOB010000000
VERSION     DAZGOB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.024"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            119..1270
                     /gene="yiaY"
                     /locus_tag="ACHLIF_001400"
     CDS             119..1270
                     /gene="yiaY"
                     /locus_tag="ACHLIF_001400"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO9605441.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1435..2973
                     /gene="aldB"
                     /locus_tag="ACHLIF_001401"
     CDS             1435..2973
                     /gene="aldB"
                     /locus_tag="ACHLIF_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO9605442.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3192..3395)
                     /locus_tag="ACHLIF_001402"
     CDS             complement(3192..3395)
                     /locus_tag="ACHLIF_001402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605443.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
     gene            3518..3841
                     /gene="yiaW"
                     /locus_tag="ACHLIF_001403"
     CDS             3518..3841
                     /gene="yiaW"
                     /locus_tag="ACHLIF_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO9605444.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3847..4983
                     /gene="yiaV"
                     /locus_tag="ACHLIF_001404"
     CDS             3847..4983
                     /gene="yiaV"
                     /locus_tag="ACHLIF_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9605445.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYKRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4980..5954)
                     /gene="yiaU"
                     /locus_tag="ACHLIF_001405"
     CDS             complement(4980..5954)
                     /gene="yiaU"
                     /locus_tag="ACHLIF_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9605446.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            6078..6818
                     /gene="yiaT"
                     /locus_tag="ACHLIF_001406"
     CDS             6078..6818
                     /gene="yiaT"
                     /locus_tag="ACHLIF_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO9605447.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
     gene            complement(6765..7028)
                     /locus_tag="ACHLIF_001407"
                     /pseudo
     CDS             complement(6765..7028)
                     /locus_tag="ACHLIF_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7032..7133
                     /locus_tag="ACHLIF_001408"
                     /pseudo
     CDS             7032..7133
                     /locus_tag="ACHLIF_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(7165..7860)
                     /gene="araD"
                     /locus_tag="ACHLIF_001409"
     CDS             complement(7165..7860)
                     /gene="araD"
                     /locus_tag="ACHLIF_001409"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO9605448.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(7854..8714)
                     /gene="sgbU"
                     /locus_tag="ACHLIF_001410"
     CDS             complement(7854..8714)
                     /gene="sgbU"
                     /locus_tag="ACHLIF_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO9605449.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(8707..9369)
                     /gene="ulaD"
                     /locus_tag="ACHLIF_001411"
     CDS             complement(8707..9369)
                     /gene="ulaD"
                     /locus_tag="ACHLIF_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO9605450.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(9366..10862)
                     /gene="lyxK"
                     /locus_tag="ACHLIF_001412"
     CDS             complement(9366..10862)
                     /gene="lyxK"
                     /locus_tag="ACHLIF_001412"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO9605451.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHTLPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(10866..11852)
                     /gene="yiaO"
                     /locus_tag="ACHLIF_001413"
     CDS             complement(10866..11852)
                     /gene="yiaO"
                     /locus_tag="ACHLIF_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO9605452.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            complement(11865..13142)
                     /gene="yiaN"
                     /locus_tag="ACHLIF_001414"
     CDS             complement(11865..13142)
                     /gene="yiaN"
                     /locus_tag="ACHLIF_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO9605453.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(13145..13618)
                     /gene="yiaM"
                     /locus_tag="ACHLIF_001415"
     CDS             complement(13145..13618)
                     /gene="yiaM"
                     /locus_tag="ACHLIF_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO9605454.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            complement(13736..14203)
                     /gene="yiaL"
                     /locus_tag="ACHLIF_001416"
     CDS             complement(13736..14203)
                     /gene="yiaL"
                     /locus_tag="ACHLIF_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO9605455.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            complement(14215..15213)
                     /gene="yiaK"
                     /locus_tag="ACHLIF_001417"
     CDS             complement(14215..15213)
                     /gene="yiaK"
                     /locus_tag="ACHLIF_001417"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO9605456.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15414..16262
                     /gene="yiaJ"
                     /locus_tag="ACHLIF_001418"
     CDS             15414..16262
                     /gene="yiaJ"
                     /locus_tag="ACHLIF_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO9605457.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            16364..16837
                     /gene="ysaA"
                     /locus_tag="ACHLIF_001419"
     CDS             16364..16837
                     /gene="ysaA"
                     /locus_tag="ACHLIF_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9605458.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHCWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(16989..18242)
                     /gene="avtA"
                     /locus_tag="ACHLIF_001420"
     CDS             complement(16989..18242)
                     /gene="avtA"
                     /locus_tag="ACHLIF_001420"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO9605459.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18420..20450)
                     /gene="malS"
                     /locus_tag="ACHLIF_001421"
     CDS             complement(18420..20450)
                     /gene="malS"
                     /locus_tag="ACHLIF_001421"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO9605460.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTETFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDMTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            20770..21594
                     /gene="bax"
                     /locus_tag="ACHLIF_001422"
     CDS             20770..21594
                     /gene="bax"
                     /locus_tag="ACHLIF_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO9605461.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSNSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(21702..22880)
                     /gene="xylR"
                     /locus_tag="ACHLIF_001423"
     CDS             complement(21702..22880)
                     /gene="xylR"
                     /locus_tag="ACHLIF_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO9605462.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(22958..24139)
                     /gene="xylH"
                     /locus_tag="ACHLIF_001424"
     CDS             complement(22958..24139)
                     /gene="xylH"
                     /locus_tag="ACHLIF_001424"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO9605463.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(24117..25658)
                     /gene="xylG"
                     /locus_tag="ACHLIF_001425"
     CDS             complement(24117..25658)
                     /gene="xylG"
                     /locus_tag="ACHLIF_001425"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO9605464.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(25736..26728)
                     /gene="xylF"
                     /locus_tag="ACHLIF_001426"
     CDS             complement(25736..26728)
                     /gene="xylF"
                     /locus_tag="ACHLIF_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9605465.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            27094..28416
                     /gene="xylA"
                     /locus_tag="ACHLIF_001427"
     CDS             27094..28416
                     /gene="xylA"
                     /locus_tag="ACHLIF_001427"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO9605466.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMAVALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            28488..29942
                     /gene="xylB"
                     /locus_tag="ACHLIF_001428"
     CDS             28488..29942
                     /gene="xylB"
                     /locus_tag="ACHLIF_001428"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO9605467.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            30111..30452
                     /gene="yiaB"
                     /locus_tag="ACHLIF_001429"
     CDS             30111..30452
                     /gene="yiaB"
                     /locus_tag="ACHLIF_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO9605468.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            30498..30935
                     /gene="yiaA"
                     /locus_tag="ACHLIF_001430"
     CDS             30498..30935
                     /gene="yiaA"
                     /locus_tag="ACHLIF_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO9605469.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(30977..31972)
                     /gene="wecH"
                     /locus_tag="ACHLIF_001431"
     CDS             complement(30977..31972)
                     /gene="wecH"
                     /locus_tag="ACHLIF_001431"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO9605470.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            32147..32446
                     /gene="ysaB"
                     /locus_tag="ACHLIF_001432"
     CDS             32147..32446
                     /gene="ysaB"
                     /locus_tag="ACHLIF_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO9605471.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLSMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            32541..33452
                     /gene="glyQ"
                     /locus_tag="ACHLIF_001433"
     CDS             32541..33452
                     /gene="glyQ"
                     /locus_tag="ACHLIF_001433"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO9605472.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            33462..35531
                     /gene="glyS"
                     /locus_tag="ACHLIF_001434"
     CDS             33462..35531
                     /gene="glyS"
                     /locus_tag="ACHLIF_001434"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO9605473.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            35856..36008
                     /gene="hokA"
                     /locus_tag="ACHLIF_001435"
     CDS             35856..36008
                     /gene="hokA"
                     /locus_tag="ACHLIF_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO9605474.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(35997..36056)
                     /locus_tag="ACHLIF_001436"
     CDS             complement(35997..36056)
                     /locus_tag="ACHLIF_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605475.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(36196..36408)
                     /gene="cspA"
                     /locus_tag="ACHLIF_001437"
     CDS             complement(36196..36408)
                     /gene="cspA"
                     /locus_tag="ACHLIF_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO9605476.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(36689..36979)
                     /gene="yiaG"
                     /locus_tag="ACHLIF_001438"
     CDS             complement(36689..36979)
                     /gene="yiaG"
                     /locus_tag="ACHLIF_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO9605477.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            37413..38123
                     /gene="yiaF"
                     /locus_tag="ACHLIF_001439"
     CDS             37413..38123
                     /gene="yiaF"
                     /locus_tag="ACHLIF_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO9605478.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(38173..39147)
                     /gene="ghrB"
                     /locus_tag="ACHLIF_001440"
     CDS             complement(38173..39147)
                     /gene="ghrB"
                     /locus_tag="ACHLIF_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO9605479.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(39251..39910)
                     /gene="yiaD"
                     /locus_tag="ACHLIF_001441"
     CDS             complement(39251..39910)
                     /gene="yiaD"
                     /locus_tag="ACHLIF_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO9605480.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            40063..42396
                     /gene="bisC"
                     /locus_tag="ACHLIF_001442"
     CDS             40063..42396
                     /gene="bisC"
                     /locus_tag="ACHLIF_001442"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO9605481.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(42365..42805)
                     /gene="yiaC"
                     /locus_tag="ACHLIF_001443"
     CDS             complement(42365..42805)
                     /gene="yiaC"
                     /locus_tag="ACHLIF_001443"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO9605482.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(42802..43365)
                     /gene="tag"
                     /locus_tag="ACHLIF_001444"
     CDS             complement(42802..43365)
                     /gene="tag"
                     /locus_tag="ACHLIF_001444"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO9605483.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            43523..44221
                     /gene="yhjY"
                     /locus_tag="ACHLIF_001445"
     CDS             43523..44221
                     /gene="yhjY"
                     /locus_tag="ACHLIF_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO9605484.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            44450..45652
                     /gene="yhjX"
                     /locus_tag="ACHLIF_001446"
     CDS             44450..45652
                     /gene="yhjX"
                     /locus_tag="ACHLIF_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9605485.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT        11191 a        11301 c        11758 g        11474 t
ORIGIN      
        1 aatgaataca atgtgctttt tattagatta attttgataa ttaataataa tgatcatgtt
       61 ttcgaatgaa aatcctcagt aagctgccca ccctttttta cactttcagg agtgtgttat
      121 ggcatcttca actttcttta ttccttctgt gaatgtcatc ggcgctgatt cattgactga
      181 tgcaatgaat atgatggcag attatggatt tacccgtacc ttaattgtca ctgacagtat
      241 gttaacgaaa ttaggtatgg cgggtgatgt gcaaaaagca ctggaagaac gcaatatttt
      301 tagcgttatt tatgatggca cccaacctaa ccccaccacg gaaaacgtcg ccgcaggttt
      361 gaaattactt aaagaaaata attgcgatag cgtgatctcc ttaggcggtg gttctccaca
      421 cgactgcgca aaaggtattg cgctggtggc agccaatggc ggcgatattc gcgattacga
      481 aggcgttgac cgctctgcaa aaccgcagct gccgatgatc gccatcaata ccacggcggg
      541 tacggcctct gaaatgaccc gtttctgcat catcactgac gaagcgcgtc atatcaaaat
      601 ggcgattgtt gataaacatg tcactccgct gctttctgtc aatgactcct ctctgatgat
      661 tggtatgccg aagtcactga ccgccgcaac gggtatggat gccttaacgc acgctatcga
      721 agcgtatgtt tctattgccg ccacgccgat cactgacgct tgtgcactga aagccgtgac
      781 catgattgcc gaaaacctgc cgttagccgt tgaagatggc agtaatgcga aagcgcgtga
      841 agcaatggct tatgcccagt tcctcgccgg tatggcgttc aataatgctt ctctgggtta
      901 tgttcatgcg atggcgcacc agctgggcgg tttctacaac ctgccacacg gtgtatgtaa
      961 cgccgttttg ctgccgcatg ttcaggtatt caacagcaaa gtcgccgccg cacgtctgcg
     1021 tgactgtgcc gctgcaatgg gcgtgaacgt gacaggtaaa aacgatgcgg aaggtgctga
     1081 agcctgcatt aacgccatcc gtgaactggc gaagaaagtg gatatcccgg caggcctacg
     1141 cgacctgaac gtgaaagaag aagatttcgc ggttctggcg actaatgccc tgaaagatgc
     1201 ctgtggtttt actaacccga tccaggcaac tcacgaagaa attgtggcga tttatcgcgc
     1261 agcgatgtaa tcatcatttc cacaacggct ggcaaattgt cagccgcttt ttcaactatc
     1321 tctgtaaccc ttgcccgtaa attcgtgata gctgtcgtaa agctgttacc gactggcgaa
     1381 gatttcgcca gtcacgtcta cccttgttat acctcacacc gcaaggagac gatcatgacc
     1441 aataatcccc cttcagcaca gattaagccc ggcgagtatg gtttccccct caagttaaaa
     1501 gcccgctatg acaactttat tggcggcgaa tgggtagccc ctgccgacgg cgagtattac
     1561 cagaatctga cgccggtgac cgggcagctg ctgtgcgaag tggcgtcttc gggcaaacga
     1621 gacatcgatc tggcgctgga tgctgcgcac aaagtgaaag ataaatgggc gcacacctcg
     1681 gtgcaggatc gcgcggcgat tctgtttaag attgccgatc gaatggaaca aaaccttgag
     1741 ctgttagcga cggctgaaac ctgggataac ggcaaaccca ttcgcgaaac cagtgctgcc
     1801 gacgtgccgc tggcgattga ccattttcgc tatttcgcct cgtgtattcg ggcgcaggaa
     1861 ggtgggatca gtgaagttga tagcgaaacc gtggcctatc atttccatga accgttaggc
     1921 gtggtggggc agattatccc gtggaacttc ccgctgctga tggcgagctg gaaaatggct
     1981 cccgcgctgg cggcgggcaa ctgtgtggtg ctgaaacccg cacgtcttac cccgctttct
     2041 gtactgctgc taatggaaat cgtcggtgat ttactgccgc cgggcgtggt gaacgtggtc
     2101 aacggcgcag gtggggaaat tggcgaatat ctggcgacct cgaaacgcat cgccaaagtg
     2161 gcgtttaccg gctcaacgga agtgggccaa caaattatgc aatacgccac gcaaaacatt
     2221 attccggtga cgctggagct gggcggcaaa tcgccaaata tcttctttgc tgatgtgatg
     2281 gatgaagaag atgccttttt cgataaagcg ctggaaggct ttgcactgtt tgcctttaac
     2341 cagggtgaag tttgcacctg tccgagtcgt gccttagtgc aggagtcaat ctacgaacgc
     2401 tttatggaac gcgccatccg ccgtgtcgaa agcattcgca gcggtaaccc actcgacagc
     2461 gtgacgcaaa tgggcgcgca ggtttctcac gggcaactgg aaaccatcct caactacatt
     2521 gatatcggta aaaaagaggg cgcagacgtg ctcaccggcg ggcggcgcaa gctgctggaa
     2581 ggtgaactga aagacggcta ctacctcgaa ccgacgattc tgtttggtca gaacaatatg
     2641 cgggtgttcc aggaggagat ttttggcccg gtgctggcgg tgaccacctt caaaacgatg
     2701 gaagaagcgc tggagctggc gaacgatacg caatatggcc tgggcgcggg cgtctggagc
     2761 cgcaacggta atctggccta taagatgggg cgcggcatac aggctgggcg cgtgtggacc
     2821 aactgctatc acgcttaccc ggcacatgct gcgtttggtg gctacaaaca atcaggtatc
     2881 ggtcgcgaaa cccacaagat gatgctggag cattaccagc aaaccaagtg cctgctggtg
     2941 agctactcgg ataaaccgtt ggggctgttc tgatataaga agctggtcgc attgggtatt
     3001 cattgcctga tgcgacgctt acgcgtctta tcatgcctac gggaacctga ccgtaggccg
     3061 gataaggcgt acacgccgca tccggcataa acaaagcgca cgttgttaca atttacgttt
     3121 taaacctacg tgcgcaataa cccagactac gccaacagaa gtgcgtttct ggggcgtgtt
     3181 ttgccaggag tttactctcg cgtttcgatg gtgagcgggc aaaccaggcc gtaaatcacc
     3241 attggcgttc cgaggccaat cagcaataaa cggtagataa ttgaaggttt aatacgcttt
     3301 atctgtactg tggatgtttt actggagtta ttcattagct tggtcaatag ttccattatt
     3361 tcaatatgat aaacctcgac ctgttgaaaa gccacgcagc acacttcaga aagtatataa
     3421 aaaacaatat gccgttatat aaataaggaa attgcactgc atcatgggat ttcataatat
     3481 gcgttaattg ttctttttta tattctctga gggaaatatg tttctggatt attttgcact
     3541 gggagtgctt atttttgtat ttctggtgat attctatggg attattattt tacatgatat
     3601 tccctacctg attgctaaaa aacgtaatca tcctcatgcc gacgccattc atgttgctgg
     3661 ttgggtgagt ctttttacgc tgcatgttat ctggccattt ttgtggattt gggccacgct
     3721 ttaccgcccg gagcggggat ggggaatgca aaaccatgac tcctccgttg tgcaactaca
     3781 acagcgtatt gccgggctgg agaaacagct cgccgacatc aaatcctctt ctgccgagta
     3841 atacttatgg atttactgat tattttgacc tatgtggctt ttgcatgggc aatgtttaag
     3901 atcttcaaaa ttcctgtaaa taaatggacc attcccacag cggccctggg tggcatattt
     3961 attgtcagtg gtttaattct gttaatgaac tataaccatc cgtatacctt caaagcgcaa
     4021 aaagcggtta tttctattcc tgttgtccca caggtgacag gcgtggtgat cgaagtgacg
     4081 gataagaaaa atacgctgat taaaaaaggt gaggtgctat ttcgactgga cccgacgcgt
     4141 tatcaggcgc gggtggatcg gctgatggcg gatatcgtta ccgcagaaca taaacagcgg
     4201 gcgttgggcg cagagttaga tgagatggcg gcgaatactc agcaggcaaa ggccacgcgg
     4261 gataaattcg ctaaagagta taagcgttac gcacgtggca gccaggcgaa agtaaacccg
     4321 ttttcagaac gcgatatcga tgtggcgcgg caaaattatc tggcgcagga agcctccgtg
     4381 aagtcatcgg cggcggaaca aaaacagatc cagagccagc tggatagcct ggtgttgggt
     4441 gaacattctc aaatcgccag cctgaaagca cagctcgcgg aagcaaaata taaccttgag
     4501 cagacgatag tgcgtgcgcc gagcgatggt tatgtgaccc aggtactgat tcgtccgggt
     4561 acctatgccg cgtcgctgcc gctacgtccg gtgatggtgt ttatacccga tcagaaacga
     4621 caaatcgtgg cgcagttccg tcagaactcc ttgctgcgac tggctcctgg cgacgatgcg
     4681 gaagtggtgt ttaatgctct gccaggtaag gtattcagcg gtaagctggc agccattagt
     4741 ccagccgttc ccggcggagc ttatcagtcg accggcacct tacagacgtt aaacacagcg
     4801 ccgggttcag atggcgttat cgcgaccatt gaactggatg agcacactga tttgagcgct
     4861 ttaccagacg gtatttacgc ccaggtggcg gtttactctg atcatttcag ccatgtctcg
     4921 gtgatgcgca aagtgctatt acgcatgacc agctgggtgc attaccttta tctcgatcat
     4981 taatcttcgc tttccgccgc ttgttcaggc cgcagttgct gcgactgagc aagctggtgg
     5041 cgaatttcac gcagcatttc atccagcaca tactggaccc gccagggctg gtattcccgt
     5101 ttacggaaga tagccaccag atccagccac cattcatagg gttgctgaaa gcagggcacc
     5161 agcgtgccgt cttgcaggtc gctctgcaca cttttgtctg gcgcaaacac aatccccagg
     5221 tgatttcgtg ccagctccag cgcagattgc gtgttgtcgc aaacgtaatt tcccttcacg
     5281 cgataatccc gcacctcttt actcccggcg acgttgaagc gccagatatt cgcgtcgtcg
     5341 atcatcatcg agtcgatcaa aatacaggag tgatgttcga gttcgtcagg acggctaatg
     5401 ggatgtttct caagatagcg ctggctggca taagcggtta cggcatattg ggtaataaaa
     5461 ctggcaacca gcgattcatc ttttggtgga gcgtagctga ttaaaacatc acaatcatca
     5521 ggaaattcga cgccttcata aaaggcatta cgctcaagat tgcaggtttt tagcgataag
     5581 gtgatatcac cgatatcttt aattttatcg attacatgtt tggataaata ggtaattatg
     5641 ccggttgggg cgtagatggt gacccgacca cgtttctcat gcttataatc tgcaataaaa
     5701 ttattaagtt gctcgtttct gtccagcatg tcgttgatgt acggtaacag cgcggtgcca
     5761 aaaggtgtga gcatcagctg ccgggtggtt cggtcaaaaa cttttaaacc cacttttgat
     5821 tcaaaatcag caagatattt gctgacgttg gcctgtgcga tgccaagtac agtcgcggca
     5881 tggctgatat tttcactggc agcgattacc gagataattt ttaactcccg gtacttaagt
     5941 tgtaattttg tcatcgcaac agcccaatta tatataattt tatatattac tatataaata
     6001 tgaatcttgc accggagaaa ttgtaagcat tattatgccg actctttttt atcttatttt
     6061 aaaatggaat accggacatg ttaattaatc gcaatattgt ggcgttattt gcgttgcctt
     6121 ttatggcaag cgcaactgct tctgaattat ccattggtgc tggtgcagct tataatgaat
     6181 cgccttatcg cggttataat gaaaatacaa aggcaattcc gctgattagt tatgaaggtg
     6241 attcttttta tgttcgtcag accacattag gttttattct gtcgcaaagt gaaaaaaatg
     6301 aacttagcct gaccgcatcc tggatgccgc tggaatttga ccctgccgat aatgacgatt
     6361 atgccatgca acagcttgat aagcgtgata gtacggctat ggcgggggtt gcctggtatc
     6421 accacgagcg ttggggaacc gtgaaagcct ctgcggctgc ggacgttctg gataacagca
     6481 acggctgggt gggggagcta tcggtattcc acaaaatgca gataggtcgc ctgtcgctga
     6541 cacctgcgct gggcgttctc tattatgacg agaatttcag tgactattac tatggcattt
     6601 cagagagtga gtcccgtcgt agcggtctgg caagttattc cgcgcaggat gcctgggtgc
     6661 cctatgtcag cctgacggca aaatacccga taggagagca cgtcgtattg atggcgagcg
     6721 caggatacag cgagctgccg gaagagatta ccaacagccc gatgattgat cgtaatgaga
     6781 gttttacctt tatcaccggc gtgagctggc gtttttaatt caccggtgga tgtcggtgcg
     6841 gctggtgctc cgtcgtaatg ccacaaaggt tgttgctctg gattgctctg ttcataccgg
     6901 gaacctggcg tctgaatcag gatcttatgg ctgaacaatc ccttgccttc aaatgtcgta
     6961 aatggcgcat cggcgggcag caacaggtta tgcagtgact cgacgttgtc ggcctgctcc
     7021 atgcaatacg gtttaatgac gttggctatt tccggcagat ttttcgcaag cacaccggtt
     7081 taacgccagc agcgtggaag cggcgttaca gcaaggaaca tatcaattcg tagtgccggg
     7141 gcgatgaagc cccggcgtga gggattactg cccgtaatag gcattggccc catgcttacg
     7201 caggtagtgc ttatccagca gttcgttttg catcgcaggg agctgcggcg caagctggcg
     7261 cgagaatagg cccatatagg cgcattcttc gagtactacg gcgttatgca cggcatcggc
     7321 ggcgttttta ccccatgcga acgggccgtg agaatgcacc agcaccgccg ggatttgtgc
     7381 cggactcctg ccacgttctt cgaaggtttc aatgatcact tcgccggtct gatattcata
     7441 ttcgccgtta atctcctctg cggtcatctg tcgcgtgcag gggatggcac catagaaata
     7501 atcagcgtgc gtggtgcccc aggcggggag atccagcccg gcctgcgacc agatggtggc
     7561 gtggcgtgag tgggtatgca caatgccacc gatttcggcg tagcgacggt aaagcgccag
     7621 atgcgttggc gtgtcggacg agggcttttt gctaccttcc accaccttgc cgctggctat
     7681 ctcaaccacc accatatcgt cggcggtcat cacgtcatac tcgacgccgg aaggtttgat
     7741 taccatccat tgccgcgttt cgtctaccgc gctgacattg ccccaggtga acgtcaccag
     7801 atggtgagcg ggaagcgcca gattcgccgc cagcacgtcg gctttcagtt gctctaacat
     7861 ataaatccag cctcctgcat acgcgcttca atccaacgcc gcgcctgaat aatctccagc
     7921 accggctctt tggctttttc ggtccacatc tcaatcagga aagaaccgcg atagttcagt
     7981 ttatgcagcg ttttgaagat gccaacgaaa tcgacgcagc cttcgccaaa cggcacgtcg
     8041 cggaactgtc cgggactttg ctcggtaact ggctgggtgt ctttcaggtg gatcgcggcg
     8101 atacggtcaa tgcccagttt cagttcggcg ggaacatcat tgccccaggc gctgaggttg
     8161 ccgacgtccg ggtagacggt gaaccacggc gaggcgagca tctcgtccca ctttttccat
     8221 ttactgatgg agttcataaa cgcggtgtcc ataatctcca ccgctagcat gacttgtgat
     8281 gccgccgcct gttcgaccgc ccacgccagc ccttcagcaa aacgttgccg ggtgccttca
     8341 tcgtggtctt cgtaatagac gtcgtagccc gccagctgga tggtgcgtat gccgagatcg
     8401 cgtgccagac gaatcgcttt gctcatgatc tcccgtgctc gctggcgtac cgcgtcgtca
     8461 cggctaccaa aaggaaagcg acgatgtgcg gacaggcaca tcgacgggat cccaacaccc
     8521 gtttcgatca tcgcggcaac cagcgaagtc ctttgtgcgg tgctccagtc aagacgtgaa
     8581 agccgttcgt cggtttcatc caccgacatt tcgacaaaat caaaaccgca gctttttgcc
     8641 agcaccagcc gctccggcca ggagagatct ttcgccagcg ccttttcata aatccctaac
     8701 ggatgattac gcacgcttgc ctccccagat agcgtcgatt tgcgcatgga aatcgccagc
     8761 cacctgcgcc ggatttgctg agcctgccag tgctcgcccg gcaataaacg ctttcacgcg
     8821 aatatcttta aacagcggca ggtcagcagg ggtaatcccg ccggtaatgg aaagctcaag
     8881 gccgatatcg gaaagcgcct tcatgcgtgc cagatcggct tcgccccact gttgcccgct
     8941 ggcctgtgca tcacgaccgc gatgataaat cgcctgccgc acgccaatac gatgccagtc
     9001 gcgggcgtcg tccagcgtcc agttaccgaa cagctcgatc tgaatttcac caccgcagcg
     9061 ttgcgccatc gcgtggcctt tttccaccgt cgcgagcggt gcggcgcaga tgatggtcat
     9121 ccagttagcg cctgcgccaa atgcctgttg cgcgagcgtt tcaccagcgt cggcgacctt
     9181 ccagtcggca acgatgattt tgtcagggca ctgttcgcgc aaggctttca ccgcgccaag
     9241 cccttcgttt aaacagagaa tggtgcccgc ttcgacaata tcgacgctgt cttttaactg
     9301 cgtcacgtcg cgctgtgcgg cttcaagtga tgagtggtcg agtgccagtt gcagaagtgg
     9361 tcggctcata atatgtgctc cttgatgcgg gcgtgaaagc cctgaagtgc ggcaatgaga
     9421 tgctgatagc gttggtattt ttgttggtaa agctgatgga cagtcatatc cggcagcagg
     9481 gtgcgcaccg ggtgctgtaa atcacgctgg gcttcgctga aatcgcgata aaccccggtg
     9541 ccgacgcggg cggcaagtgc tgcaccaaag cagccggttt cttccacctg cggcagctca
     9601 atacgcagac cgctgacgtc cgccagcatt tgcatccaga catcggaatg cgccgggccg
     9661 ccagtgacgc gcagggtgtg cacatcagta aaacgttcgc gcatccggtt gagatgggtc
     9721 atatggctga acaccacccc ttcataaatg gcctgcaaca ggtgtgcgcg ggtgtgaatg
     9781 gcctgcatcc cgtagaaacc gctggtcatc tcgagtccgg cgttgctgcc gtacaggaac
     9841 ggcaggaaaa agagatcgcc accggccttc ggcaagctgg caacggcctg gttgatctca
     9901 tcaaacgaga tttctcccca ctgtgcggta aaccattcga ggttgccgga agaggtgggg
     9961 ctggcttcgt gaacgataaa ttcaccatcg ttaacgtagc gaccatagac atacggatgc
    10021 gcttcaccgt cacgtaaacc gcgggttatg ccgctggtca ccgcccaggt gcccatcacc
    10081 gcattaaggg taaattcgtc ttcgatcccg gcgcagagtg cggtggaaac cacatcaaac
    10141 aggccgccga caacgggcgt acccgctttc agaccggtca gtacggctgt ctgagcggtg
    10201 atctccccgc agatttcggc agatccgaca acaggcggca gggtgtgatt gatttcagca
    10261 atccccagcc agtcggtgag gcacgggtca tattccccaa gactcatgtt gtagagattg
    10321 gactcggaaa tattgctctc ttcacagcct ttgacgccgg ttaaacacca gcgcaggtag
    10381 tcgtgcgtca tcatcacgca gccaatttgc gcgtagcgtt ccggttcgtg ctctttaagc
    10441 caacgtaaca gcgataccgg atgcccggtc cacaaggttt gtcgggtcag cgggtagagt
    10501 ttttccggga tgccatcttc ctgccagcga cgaacgattt ccatcgcccg gcgatccgag
    10561 gataaaatgg cgttaccgag cggcttgttg tttttatcca gcaaaaacaa gccttttcct
    10621 tgtgcggaga taccgatacc gacaatttgt tccccgctaa cgccggaatg ggtaagcagt
    10681 tcgcgaatga cagccatgca gcattgccac agttctgcca tatcgcgctc tgcccaacct
    10741 ggctgcgggc ttaatgcgca cagcggcagg cgctgcacgc ctgcctcccg gccttcgcgg
    10801 tcatacagcc cggctttcag ccagctaccg ccacaatcta accccagcca gtattgcgtc
    10861 atggtttatt gcacctcatc cacggctttc agtagatcag caccctgcgg aacatctttt
    10921 acaaacatgt cgcggacctg agtacccagt gcgtcgctaa aggctttgcg gtcgagatcg
    10981 gtgatgactt ccacgcccgc ttctttcatg ccgtcgatga ttttttgctg atcttcggca
    11041 accagtttgc gctggtagtt acccgcttcc tgcgcagatg aaattagcgc ttgctggaac
    11101 tccgggctta agccatcaaa cttcgctttg ttgatcacca ccagaagcgg ggaataggcg
    11161 tggtgcgtca gagaaaggta cttctgcact tcgaaaaatt ttgctgacca gacgacgttg
    11221 atcgggtgtt cctgagcgtc gatagtgcgg gtttccagcc cggtatagac ttcggcaaac
    11281 ggcatcggga tcgggttagc gccaaagact ttgaatgcgg cgatattcat cgggctattg
    11341 ttggtgcgaa ttttcagccc tttcaggtcg gcgggggttt taaccggtgc gcgcgagttg
    11401 gtgacatcgc gccagccgtt ttcccagtag gccagtactt ttagtccttt accttcaagt
    11461 gaggctttca gatcatcacc gactttgccg tcgagcgttt tatgcgcgtg agcggtatcg
    11521 cggaacagga aagggacatc aagcaagttc atcactggtg ataatccggc aaagttattc
    11581 gagccggaca tttccatatc gatggtgccg ccacgtacgc cgctgatcat cgcctgcgcg
    11641 ttaccgaggg tgctatccgg aaacaatttt aatttcagct cgcctttggt tctctcctgc
    11701 aataaatcat tgaatttttt cgccgcaata tgttgcgagt cggtttgtga ggtttcataa
    11761 ccgaaacgta aagattgcgc cgccagagaa gatgtgctga atgcagccag gccagcaatg
    11821 aataatgcgt aggttacaga gcgtaatttc ataatatttt ccttttaatt aatccatttc
    11881 aaagggagga tgatgagatc gggaataaaa acaaatacga ctaataatga atataaaacc
    11941 aggacgtaag ggaaaacgcc tctgactgca tcatcgaatt tgagttttgc cacccctgaa
    12001 ataacgttaa gtacattacc gataggcggt gtaattaagc cgattgagca gttaatgata
    12061 aacatgacac cgaaataaat cggatcgatt cctgcctctt taactaaagg cattaatacc
    12121 ggggtaagaa ttaataccgt cggcgttaaa tccatgacca tgccgacaat caggatagcc
    12181 accataatga cgataaacag cagacgcggt gaatcgacca acggttgcag taaatcagaa
    12241 accatcatcg gcagttcagc aatggtaatc agccaggcgg aaacttgtgc cgaggccacc
    12301 agaaacataa ccactgaggt ggttttcgcc gcgccaatga gtacatgcca aagtgtggcg
    12361 aaggtcattt cacggtaaat aactgtggcg acaaacagcg catagaaagc agcaacggcc
    12421 ccggcttcgg taggggtaaa cagaccggag cgaaacccgc caataatgat gactggaaga
    12481 aacagcgccc agataccgga gacaaaagag tgccagattt cctgcatcgt tgctttttgc
    12541 tggcggggta aattaaggcg actggcctgc caccaccagg taagcatcag cgttgcgccc
    12601 atcatcatgc caggggcaat gcccgccata aacagcttac tgatggataa tccgctggaa
    12661 acgccgaaga taataaacgg aatggaaggg ggaataattg gcgcgataat gccgccagag
    12721 gcaatcagcc ccgccgcccg gttgaccggg tagttggcgc tgcgcatcat cggcaccagt
    12781 agagcggcaa cggcggcggt atccgcaacg gcagaaccag aaaggctcgc cataatcata
    12841 gctgccagca cgccgacgta gcccagcccg cctggtttat gccccaccag tttcatcggc
    12901 aggtcaacaa tgcgttttga caagccgccc gcattcatga tttcacccgc cagcacaaag
    12961 aacggaatcg ccagcaggga gaagctatcg gctccgttca ccagcgtttg tgccatgatc
    13021 tggacatcaa acatgtccag ccagaacatt aacgccgccc cgcacaacaa cagtgcccag
    13081 gcaataggca aaccgatagc aataccaccc aacagacagc ccagaaaaat cagcacagcc
    13141 atgattaagc tccttgcggt ggcgacgtta gagaattgct acgcgtgatg agttgatata
    13201 aatgacgcag ttcaaagaat gcgataacga ggctggtggg cagacaagcg gcatacatca
    13261 ggccgatggg taaaccgagg atcggtgaat aatcgctcca gtcctgaatt gtttttagcg
    13321 ttgcgcccca ggccagtgcg ccacaaataa ataagattaa ggaatgggta accagagcga
    13381 ctcgtcgttg ccatgcgggg gagagtttct ccaccagaaa ggtgacctga acgtgggcgt
    13441 tatccataaa agccacaatc gcgccaataa atgttaacca gacaaataaa taacgtgaca
    13501 attcatctac agataaaatg cttgtctgaa aaccatatct taaaataatg tttataaaca
    13561 caatacagga aagtacggcg agattaatcg ccagtattgc ttcaagtatt tttttcatag
    13621 ctattccttg aggctatgtc tgtcataatt caagttgcat gtgcagcaac ctaaattatt
    13681 aatatagtta gagcaataac attacccagt cgttctggcg tagacgatta ttcgattaat
    13741 tcagcgccgt taatgcgact ttaaccacaa tttttcgtat ctcagaggct gtttgcaaaa
    13801 tacaacctgg tcggtgaaca tcctgcggaa agaaaattgc gtagctgccc ggtatcattt
    13861 ctataaatga ttcatgttca ctgtcatgat aaaaaataat atcgcgctgc tctaatagtg
    13921 attcgctgac tttattattt cccgtatcaa tagcaatgcc gattttctct tcaccccacg
    13981 ccagaaactg aatatcgata taccgacgat gcacttccgg acggttttcc accgcttcgc
    14041 gtgtggttaa atcgataatt tgcgcataaa tatttttgcc gtcgatttcg acaacgcccg
    14101 gctccagggc gttgaaatcg gtagcgcgca gaaaatcgag cgccttttca atggcggcgg
    14161 gcaaacggca cggattgggc tgcgcgatat gtccaaaaat catgacttat ctccttataa
    14221 cgcctggatt ttggcccaca cgctgtcatc aacggtaata ccgttacggc ggttttcggc
    14281 cagcagggta gtaaattcgt ggccgggtaa gcggatggcc tggttttcgt cagcgcgttc
    14341 agcggtagta acgtaatcca tgatacgttg cagcttggca tcgcgggtgg gaccgtcgat
    14401 aagcttgtcc acttcaatgg caataaaaat ttgtgaaatg ccgtattcgt cgctgttgtc
    14461 ctgggtgact tcggcaaccg atgcgccgtc ggaaaggaga gtagcgatca tatccagcac
    14521 aatcgacatg ccagaacctt tccagtagcc catcggcaaa atgcggcgat tcttctcgat
    14581 aacgccaggt tctttggtca aattgccctc atcatcaaag ccaccatcga ccgggagctg
    14641 acggcccgcc aggcggttaa cttctaacat gccgtaagag aacatcgaca tcgacatatc
    14701 gaccatggtg atcggcgtgg aaggaatggc gacgatcagc gggttagtgc caatgcgaca
    14761 ctcttttgcg ccccacggcg gcattacggc gatggagttg gtccagcaaa tgccaatata
    14821 gcctttttcc gccgcctgcc agccgtagct gccgccgcgc atccagtggt tggcattacg
    14881 aagtgccacc agaccaatac cgtgatcggc agccagttca atggcgcgat ccatcatctt
    14941 tttcgccgtc aggttgccga tcgaacgctg ggcatcccac tgttcaattg cgccgaggct
    15001 ggttatacgt ttgggttggg catcaggaat gatatcgccg ttttccagtt gttgaatgaa
    15061 acgagggaaa cgattaacgc cgtgagaata aacgccggat tcggtggtgc gggcgaacat
    15121 ctctgcacag gcgtcagccg tttcgctgtc aacgccgcgt gaaattaaga cccgattaaa
    15181 ggctgctttt aactgctcaa atgtcacttt catcccaggc ttccttgttt ttttgatcgc
    15241 ttttttggct taaaatttca atatgcgaaa ctttatttca aatatatcga tcacttttta
    15301 acaaagcaat ctgattgatg aatttcaaaa gacataaaaa tcaagtggtt ataaattatc
    15361 tgtcgagatc gtgaactacg gcacactttg cgctaccatc aggacgcgac aaaatgggga
    15421 aagaagtgat gggaaaaaaa gagaacgaga tggcgcagga aaaagagcgt ccagccggaa
    15481 gccagagtct gtttcgcggg ttgatgctga ttgagatttt gagcaactat ccaaacggtt
    15541 gtccgttggc gcatctttcg gagctggctg gtttaaataa gagtaccgtc catcgcttat
    15601 tgcagggatt acagtcctgc ggctatgtga ccaccgcgcc cgccgcaggg agttaccgcc
    15661 tgactaccaa atttattgcc gtcgggcaga aggcgctgtc ttcgctgaat atcattcata
    15721 tcgccgctcc gcatcttgag gcactgaaca tcgccactgg tgaaaccatt aacttctcca
    15781 gccgcgaaga cgatcacgct attttgattt ataagctgga acccacaacc gggatgctgc
    15841 gaacccgtgc ctatattggc cagcatatgc cgctctactg ttccgcaatg ggcaagatct
    15901 acatggcgtt tggtcacccg gactacgtga agtcatactg ggaaaaccat cagcatgaga
    15961 tccagccgtt aacccgcaat accattaccg agctgcccgc gatgttcgac gaactggcgc
    16021 acattcgtga aagcggagcg gcgatggaca gagaagaaaa cgaactcggc gtctcctgta
    16081 ttgctgttcc ggtgtttgat attcatgggc gggtgccgta cgccgtgtcg atttcgcttt
    16141 cgacatcacg tctgaaacag gtgggagaga aaaatctcct gaaaccactg cgtgaaaccg
    16201 cgcaggctat ttctaatgaa ctgggattta ctgtcaggga tgacctgggc gcgatcacat
    16261 aacgctttgg acaagtgcca aaactttaac atttccttcg ttggatcaaa gcagtaggga
    16321 cgcgctctct ggcactctgc tgttttagtg caaaggagtg atcatgaacc ggtttattat
    16381 tgcggatgcg acgaaatgta tcggttgccg tacctgtgaa gtggcttgcg cagtgtcgca
    16441 tcaggagaat caggattgcg ctgcgttgtc accagacgag tttatttccc gtattcgtgt
    16501 cattaaagac cactgctgga ccacggcagt agcctgtcat cagtgtgaag atgcaccgtg
    16561 cgcgaatgtc tgccctgttg acgcgataag ccgcgaacat gggcatattt tcgttgaaca
    16621 aacacgttgc attggctgta aaagctgtat gctggcttgc ccgtttggtg cgatggaggt
    16681 cgtttcttcg cgcaaaaagg cgagggcgat taagtgcgat ctgtgctggc atcgggagac
    16741 gggaccggcc tgtgttgaag cctgcccgac aaaggcgttg cagtgcatgg atgtcgagaa
    16801 agtgcagcgg catcggctac ggcagcagcc tgtttgaaac gtttttccgg cgtttctcaa
    16861 cgccggaata gtgcgctttg tttatgccag atgcggcgta aacgccttat ccggcctaca
    16921 aattcttcca aattcaatat attgcagaaa tcatgtaggc ctgataagcg tagcgcatca
    16981 ggcaattttt agtgactttc agcccaggct ctttctatct cttccgccag aatcttcacc
    17041 cccgcctcaa ttttctccgg ctctggtacg tagttcatgc gcatacattg atgcgtatgc
    17101 ggccacggtt tatccagccc tgggaagaag ttgtgccccg gcaccatcag cacgccgcgt
    17161 gctttcaggc gctgatagag ctgctcggtc gtaatgggca aatccttaaa ccatagccag
    17221 aggaaaatgg ctccttccgg tttatgaatc aggcagcgat tttccggtaa atagcggcga
    17281 atgatggcga tagtttcctg aacacgctgg tagtaaaacg gtttgatgac tgtttcagac
    17341 aggcgcagca gatcgttacg cttaatcatt tcacacatca tcgccggacc aataccgcca
    17401 ggtgccaggc tgataatgcc gttcatattg gtgatggcgg tgatgatttt ttcattggcg
    17461 atgataatgc cgcagcggga gccaggcagc cccagcttgg aaagactcat gcacagcacg
    17521 atattcggat tccatagcgg gcgcgcttca ctgaagatga tacccgggaa cgggacgcca
    17581 taagcgttat caatcaccag cggaatgccg tgttgattcg ccagcgcgtc aagcttcagc
    17641 aactcttcgt cagtaatcac attgcctgtt ggattcgtcg gccgggagac gcaaatcatc
    17701 ccggtttctt cgccaatatg cagatgctca aaatcgacgt ggtatttaaa ctggccttcc
    17761 ggcagcagtt caatattcgg acgcgcagag acaaacagat cttcttccag tccggcgtca
    17821 gcgtagccaa tgtattccgg tgccagcggg aacagcactt ttttgacccg accatcggca
    17881 cggcgtccgg caaacagatt aaataagtag aaaaacgcgc tctggctgcc gtttgttagt
    17941 gcaatattct gtggttcgat atcccaaccc aacttctcgc gcagcattcc ggcaagcagt
    18001 gtgagtagct ccgttttccc ctgtggaccg tcgtaattgc acagtgcatc agtcgctttg
    18061 ccactttcca gcatgtcggt cagtagcgtc tggaagtagt cctgcatttc cgggatctgc
    18121 gccggattac cgccgccgag cataatcgcg ccaggggtgc gtaaaccgtc gttcagatct
    18181 tccatcagca gcgtaatgcc ggagtggcgg gtaaatttgt caccaaaaag ggagaatgtc
    18241 atagcgggat atctgtcgaa ctctgtagca aggaaggtaa caataacgct acatttgctg
    18301 gggtgcaaat cggtctgttg aagagtgagc ggtgctttat cctgcaacgc tgattagggc
    18361 tgacatttta tcctggttgg cggcttcccg aggtactgac gaacgggaag ccggaaaagt
    18421 cactgttgcc cagcccagat gaccagcact ttatcgtcgc catgctcacg aacaaagccg
    18481 tagccctgct tcagcgaaag tgtcgtttgt ttgcccgcgc caattgcggg atggcgggcg
    18541 cggaactggc tgattttctg ccagtgcgcg acgttggcgg cagatttacc gctaacatcc
    18601 tgccagttca tatccgaacg tgtaccttgc agcggatcag aacctgtagg accgaacgga
    18661 cgcgaggatt catcgccata aaagatttgt accgcgcctg gcgctaatag taataactct
    18721 gctgctttgt cgcccccttc acggaacaga cgggtatcat gcgacgagag gtagctcaac
    18781 acgttgaaac cctgcaattt ctccgccatt tgctgccagg tcatatccat ctgcgcaata
    18841 caatcgacag ctttcgccgc ctgctcctga taatcgaaat tgatcatcgc atcgaagccg
    18901 tggcgatagt agtcactttg catcacgccg tggccccagg cttcaccggt catccagaaa
    18961 ggtttgtcat ctaatgcttt gtcggggtta gcttttttcc attcgcgaag cgcggcgctg
    19021 gcttcggttt tcagttgctg ccaggcgggc aactcaacat gtttggcggt atcgacccga
    19081 aaaccatcaa tcccatagtc gcggacccac tgacttaacc agtgggttaa gtaatcgcga
    19141 ggggtaaagc cgtcgatggc tttagcgtgg gtatccgttt tgtttttata gaacaccggc
    19201 agaccagaag cggtagttga ttcggttttg atatccggca aaaaggctag cgacatggtg
    19261 agatcgtcga atccaggatt gtcgtaatcg ccgatatcgg tacggatcca gttttttccc
    19321 caccatttat cccagcctgt tttgtcgctg aaattaatgt aatcgttaaa gctatgccag
    19381 gtttgcccgg cggcaggttt ccagtcgctc cagcgttcac ccagcgtttt tttcacctcg
    19441 tcaccagaaa gatataacgc gccaaactga tactcctgca tatccgccag cgtggcatag
    19501 ccggtgtggt tcatcacgac atcaaagaga atacgaatac cgcgctgatg tgcgctatca
    19561 accagcgtcc gtagatcggc ttcgttgccc atattggcat caagattcgt ccagtcctgt
    19621 gtgtaataac cgtggtaggc ataatgcggg aaatcgcctt ttgtaccgcc gccgacccag
    19681 ccgtgaattt gctcaaatgg ggcgcttatc cataaagcat taacgcccaa ctgctggagg
    19741 taatccagtt tgttggtcag gccgcgtaaa tcgccgccgt gaaaagtgcc aatttccgcc
    19801 ataccgtctt tatgacgtcc gtaactctgg tcattactgg gatcgccgtt ttcgaaacga
    19861 tctgtcagca caaagtaaac cgtggcgtta tgccagtcga aaggggcaga ggcgtcagtt
    19921 tctgcccgtt ccagcaggag taaaccgttg ctggtggcag cgggttgtaa cgttatttga
    19981 ccgttcttca ctatcgcaat ttgctggctg taataatccc gtacggcggc tccttccggg
    20041 aaagtggcgc tgacatccag cgtgagcggt aatccatccc atttcgggca tacacggacc
    20101 tggcttgcta ccggttcggc ggcgttctgg atggaaatca tcaatgttgg cgtgccggag
    20161 cgggtgtcta tttgcagcgt atattcgccg tccctgaaca atcgccattg aggctgcgtg
    20221 ttgctacaag gttgcaggga aagcatctga ttgagtttta tcgcatccgc aggctgccag
    20281 cactgttggt caaaatttag cgttagtgga cgcgtacctt tgggcaactg cgcgtggctg
    20341 acaaatgttt ccgttccctg ttcactaaag gcagggaacc ccggagaagt ccagctggcg
    20401 gcaacggcga agccaggaag gagtgtcaga aaacaggcgg cgagtttcat ggcggatgag
    20461 tccttattgc tgcgatttca gacagtttgc cagcgataag ccaaacaaaa ctcatccctg
    20521 agcggtaagt ccacaggatg agaagcaagg gtgagcgatc tcgcgcaaaa aacggctgaa
    20581 ttttgcgata aaccccacat tttctgcgat ttagcgccaa tctgaatcgt taactcgtga
    20641 catagtttca gatttggact attccttggg gtgctcttga cagctatttt tgatatgatt
    20701 tgagattcaa ctctcaaatt tgtgaaaaat ataaggtgtt ggaatgatta aatccgacca
    20761 ggagacctga tgattttgac tcccatacga cgatatgggg cgatgattct tatgttactc
    20821 actctggtgt tttcgagtga ggtgttagcg aagacgcaca caacaacagc gagtcaaaag
    20881 tcccacttaa ctaaagctag taataaacag gtaagcagta aacaagagta ttctcgcaat
    20941 agtgcaaaga gtaattcact tcctgatttg cgaaaatacc cttccggaac accaaggaaa
    21001 aaggcgtttc tccggaccgt aatgccttac attaccagcc aaaatgcagc cattactgcg
    21061 gaacgtaact ggctgatttc aaaacagtat cagggccaat ggtcacctgc tgagcgtgcg
    21121 cgtctgaaag acatcgccaa acgctacaag gtgaagtggt ctggtaatac gcgaaaaatc
    21181 ccgtggaata ccttgcttga acgcgtagac attatcccca ccagtatggt ggcgacgatg
    21241 gctgcagcag aaagcggttg gggaacgtca aagctggcgc gcaacaacaa caatctgttc
    21301 ggcatgaaat gcatgaaagg acgttgtacc aatgcgccgg gtaaagtgaa agggtactca
    21361 cagtttagtt ctgtcaaaga atcggtgagc gcctatgtca ctaacctcaa tacgcatccg
    21421 gcttactctt cattccgtaa atcgcgtgcg cagctgcgta aagcggatca ggaagtgact
    21481 gccacagcga tgattcacaa gctgaagggc tactcgacca aagggaagag ttataacaac
    21541 tacctgttcg caatgtacca ggataaccaa cggttaatcg cggcgcatat gtaattgcat
    21601 ttccttgagc cttatctgat ttgtcggtcg gataaggctt ttgctcgcat caggtggctg
    21661 tgctgagttc cctgatgtga cgccgacaat tctcatcatc gctacaacat gacctcgcta
    21721 tttacatcgc gatactcttt tggcgttgtg tcatatgctt ttttaaaaac agagtagaaa
    21781 tattgcagcg atggataacc gcacatttgc gatatctcat tgatcgacaa ggtggttgaa
    21841 atcagcagac tgcgcgcttt ctccagcttc tcggcatgaa tcattgcatg gatggtttca
    21901 cccacctctt ctttaaaacg cttctcaaga ttggagcgcg agatcccgac cgcatccagt
    21961 acctgatcca ctttaatccc tttacaggcg tgattacgaa tgtaatgcat ggcctgaata
    22021 acggcgggat cggtcagcga gcgataatct gttgagcgcc gttcaatgac gcgaactggt
    22081 gggaccaaaa ttcgctgtag cggcatttct tctttatcta ataatcgatg caacagtttt
    22141 gccgcctgat agcccatttg ccgcgcgccc tgagcgaccg aagaaagggc gacacgcgac
    22201 agatagcggg tcagttcttc gttatcgatg ccaatcacgc ataatttttc cggtacggga
    22261 atatgtagat gttcacatac ttgcagaata tgccgcgccc gggcgtcagt aacggcaata
    22321 atcccggttt gcggtggcag cgtttgtagc cagtctgcca gccgattttg cgcatgttgc
    22381 cagttctctg gcgcggtttc taacccctga taaaccactc cgcgatactt ttcttcggcg
    22441 acaagctgac gaaatgcata ttcgcgttca gtggcccaac gtttgccgct tgattccgga
    22501 agaccataaa aagcaaagcg gttaacgcct ttctctttta aatgcaaaaa tgcgctttca
    22561 accagcgcat agttatcggt ggcaatgtaa tgaacgggtg ggtaactttc tgcaaggtga
    22621 tacgagccgc caaccccaac aatggggacg tcgacatcag ccagcgcttg ctcgatctgt
    22681 ttgtcgtcga agtcggcaat gacgccatct cctaaccagt ccttgatttt atcaatgcgg
    22741 gcgcggaaat cttcttcaat gaaaatatcc cattctgatt gtgatgcctg taaatattcc
    22801 cctacgcctt ctactacctg ccggtcatag gctttattgg cattgaacag taatgtgatg
    22861 cggtgacgtt tagtaaacat ggttcttttc ctgctgaatc atgcaaaaac tcaaaaccgg
    22921 taatacgtaa ccggctttga gaaaattttt atcaaaatca agaacggcgt ttggttgcgg
    22981 agtccatcca tactgccagc aacagaatcg cacctttaac gatatactgc cagaaggtcg
    23041 gtacatccat catactcatg ccgttatcca gtgaagccat gataaatgcc cccattactg
    23101 ctccggcaac gcttcccaca ccgccagcca ggctggtgcc gccaatcacg catgctgcaa
    23161 ttgcgtccag ttcggcgata tttcccgcag aaggtgaacc agcgccaagt cgagaactaa
    23221 ggattaatcc ggcgatggct accattaatc cgttaatcgc gaacacggca agtttggtgc
    23281 gttcaacgtt aatcccggag agacgtgctg cttccagatt gccgccgatg gcataaatgc
    23341 gtcgtccaaa tgccgtccgc gttgccataa acattccgcc gagtaacagc aatgtcagca
    23401 gcagaacagg agtgggaacg ccacggtaat cattcaacag ccagattgcg cctaatacga
    23461 tgatagcggt taaagcctgg cgaccgacta ctgcggtaga ggccggagac tgcaaaccca
    23521 aagcctgacg gcgcattctt ccgcgccatt gccaaccaac aaaagccatt aagccaagcg
    23581 cgccaatgat gaagccagtg ctggcaggta gatagctttg cccaatttgt gacatcgcgg
    23641 cgctggtggg ggaaacagtc gtgccgttgg tgatgccaat gagtatgccg cgaaatgcca
    23701 acatgcccgc gagggtgaca ataaatgaag ggactttgcg gtacgcgacc caccatccgt
    23761 tccaggcacc gagaagcagt cccagaacca acgtcacaat gatggtaagt ggcaaaggcc
    23821 agcctaacca gacgtcacaa atcgccgcga cgccacctaa tagccccatc attgagccga
    23881 cggaaaggtc gatttcagca gaaattatga cgaacaccat tcctaccgcg aggatgccgg
    23941 taatcgcggt ctggcgtaac aggttggaga cgttacgggc gcttaagtag gcaccatcgg
    24001 tggtccaggt aaagaacagc atgattgcga tgatagctgc aatcatcacg aagacctgca
    24061 aattcagtga tttcagcccg gaaaagccac cggatgtcgg tacggccaat ttcacttcag
    24121 acggattgct tttcgacatg atgttcgctc ctcaatgcgg cttccatcac ctgctcctga
    24181 gtcaggttat gatttatcag gttggctttt agtttccctt catgcatcac cagtacccga
    24241 tcgctaaggc cgagcacttc aggtaattcg gaagagatga caataacggc aataccctgc
    24301 tggacgagtt ggttaattaa tttgtagatc tcgtatttcg cgccaatatc gatacccctg
    24361 gtaggttcat caagaatgag gatgcgcggg ttaagtaaca gacagcgagc gaggatcgct
    24421 ttttgctgat tgccgccgct caaacgtcca atagcaagat cgggggacga cgttttaact
    24481 ttgagttgct gaattgattc cagaatacat ttttgctctt ccgcgtcatc aagctggcta
    24541 atgccaccgg taaatttatt gagtgcggcg agggtaatat ttttaccaac cgccattacc
    24601 ggaacgatgc cgtcgcgctt tctgtcttcg ggtaccatcg caatcccctg ggcgatggct
    24661 tgctgacagt tacgaatatc tacctgtttg ccatcaatat aaatttttcc ttcccattgt
    24721 ccgggccaca cgccaaacag gcactgaatg gtctcggtac gtccggcacc aacgagtccg
    24781 gcaataccca gtatttcgcc acgtttcagg gaaaacgaga catcattaac tcgtttaata
    24841 tgacgattga ccggatgcca tgccgtcaga tgttcaatac gtaatatttc atctccggtg
    24901 gtatgtggtt cattagggta aagcgcggtt aactctcgcc cgaccatcat ggtgataata
    24961 tcgtcttcac tcattccggc agcatcacgc gtaccaatgt gctgtccgtc gcgaataacg
    25021 caaatcgtat cggaaatcgc tttgacttcg ttgagtttgt gcgaaatata aatacaggcg
    25081 ataccgtgct gttgtagatc gcgaataata tccagtaaaa ccgacgtttc ctgctcagtt
    25141 aatgaggctg tcggttcatc gagaattaac aagcgcacct gtttattaag tgccttggca
    25201 atttcaacca gttgttgttg cccaagccct aaatcgccaa cgcgggtatc aggtgaaatg
    25261 gataaactga cctgtgcgag cagcttctga cagcgtagcg tcatcaggtc ataatccata
    25321 atgccattgt gggttatttc gttacccagg aagatatttt ccagcacggt caattctttc
    25381 accagggcca attcctggtg aatgatggcg atacctttgc gttcggtatc gcggatgtga
    25441 ctcgcctgaa tctcttctcc cgcaaaaata atttcgcctt cgtaggagcc atggggataa
    25501 ataccacaca gcactttcat cagcgttgat ttaccagacc cattttcccc acaaagtgag
    25561 acgatttcgc cagcattcaa ccgcaagcag acgttatcaa tcgccttcac actgccgaag
    25621 gttttggtaa tattcttcat ttcaagtaga taaggcataa cgactccacc taagccaatt
    25681 cattcacgcg gcatggagag aaatcacgcc cccgctccgc gccggggcgt aacgcttaca
    25741 gctcgctctc tttgtggaat ccgtctttaa ctaccgtgtc tttgatgttg tttttattca
    25801 catcgatcgg tgtcaggagg cgggagggca catctttcag gccattattc agtgtggtat
    25861 ctgcttttgg ttcctgacca ttgcccaact caacggcaat ttctgcggca gtatttgcca
    25921 acaacgtaat aggtttatac accgtcatag tttgcgtacc ggcagcaata cgtttaatac
    25981 ctgcgagatc cgcatcctgg ccggaaattg ctactttccc tgataaacct tgcgcgctta
    26041 atgcctgaat tgccccacct gcggtggcat cgtttgaggc aactacagca tcaattttgt
    26101 tattattggc ggttagcgcg ttttccataa ttttcaatgc gttttccggt aaccagccat
    26161 caacccattg gtcaccaacg actttaattt ttccggaatc aacgtaaggt tttaacactt
    26221 tcatttgtcc ggcgcggaac agcttggcgt tgttatctac cggcgagccg cccatcagga
    26281 agtaattacc ttgcggaaca atatcgacca gggcttttgc ctgcagttca ccgacttttt
    26341 cgttatcgaa agaaatataa aaatcgatat ccgcatcgtt aatcatacga tcgtaagcta
    26401 atactttaat accttcttgt ttggcttctt ttacaacgtt acttaatacc tgaccgttat
    26461 acggaataat gacaagaaca tcaacacccc ggtttatcat gttttcaatc tgcgacattt
    26521 gtgtttcttc attgccattt gcagactgta caaatacttt cgcgccgaga gattctgcct
    26581 ttttcacaaa aatatctcga tctttttgcc agcgttcaag acggagatca tcaatcgcca
    26641 tacctatttt gacttctttg gcgtgtgccg caacgttggt aagcagaagt gaggtgcaaa
    26701 gggtgagtag aatgttcttt attttcatgg tgtagggcct tctgtagtta gaggacagct
    26761 ttaataagta acaatcaccg cgataaacgt aaccaatttt tagcaactaa acaggggaaa
    26821 acaattacag atttttatct ttcgattacg atttttggtt tatttcttga tttatgaccg
    26881 agatcttact tttgttgcgc aattgtactt attgcatttt tatcttcgag gaattaccca
    26941 gtttcatcat cccattttat tttgcgagcg agcgcacact tgtgaattat ctcaatagca
    27001 gtgtgaaata acataattga gcaactgaaa gggagtgccc aatattacga catcatccat
    27061 cacccgcggc attacctgat tatggagttc aatatgcaag cctattttga ccagctcgat
    27121 cgcgttcgtt atgaaggctc aaaatcctca aacccgttag cattccgtca ctacaatccc
    27181 gacgaactgg tgttgggtaa gcgtatggaa gagcacttgc gttttgccgc ctgctactgg
    27241 cacaccttct gctggaacgg ggcggacatg tttggtgtgg gggcgtttaa tcgtccgtgg
    27301 cagcagcctg gtgaggcact ggcgttggcg aagcgtaaag cagatgtcgc atttgagttt
    27361 ttccacaagt tacatgtgcc attttattgc ttccacgatg tggatgtttc ccctgagggc
    27421 gcgtcgttaa aagagtacat caataatttt gcgcaaatgg ttgatgtcct ggcagccaag
    27481 caagaagaga gcggcgtgaa gctgctgtgg ggaaccgcta actgctttac aaaccctcgc
    27541 tatggcgcgg gtgcggcgac gaacccagat cctgaagtct tcagctgggc ggcaacgcaa
    27601 gttgttacag cgatggaagc aacccataaa ttgggcggtg aaaactatgt cctgtggggt
    27661 ggtcgtgaag gttacgaaac gctgttaaat accgacttgc gtcaggagcg tgaacaactg
    27721 ggccgcttta tgcagatggt ggttgagcat aaacataaaa tcggcttcca gggcacgttg
    27781 cttatcgaac cgaaaccgca agaaccgact aaacatcaat atgattacga tgccgcgacg
    27841 gtctatggct tcctgaaaca gtttggtctg gaaaaagaga ttaaactgaa cattgaagct
    27901 aaccacgcga cgctggcagg tcactctttc catcatgaaa tagccaccgc cattgcgctt
    27961 ggcctgttcg gttctgtcga cgccaaccgt ggcgatgcgc aactgggctg ggacaccgac
    28021 cagttcccga acagtgtgga agagaatgcg ctggtgatgt atgaaattct caaagcaggt
    28081 ggtttcacca ccggtggtct gaacttcgat gccaaagtac gtcgtcaaag tactgataaa
    28141 tatgatctgt tttacggtca tatcggcgcg atggatacga tggcagtggc gctgaaaatt
    28201 gcagcgcgca tgattgaaga tggcgagctg gataaacgca tcgcgcagcg ttattccggc
    28261 tggaatagcg aattgggcca gcaaatcctg aaaggccaaa tgtcactggc agatttagcc
    28321 aaatatgctc aggaacataa tttgtctccg gtgcatcaga gtggtcgcca ggagcaactg
    28381 gaaaatctgg taaatcatta tctgttcgac aaataacggc taactgtgca gtccgttggc
    28441 ccggttatcg gtagcgatac cgggcatttt tttaaggaac gatcgatatg tatatcggga
    28501 tagatcttgg cacctcgggc gtaaaagtta ttttgctcaa cgagcagggt gaggtggttg
    28561 cttcgcaaac ggaaaagctg accgtttcgc gcccgcatcc actctggtcg gaacaagacc
    28621 cggaacagtg gtggcaggca actgatcgcg caatgaaagc tctgggcgat cagcattctc
    28681 tgcaggacgt taaagcattg ggtattgccg gccagatgca tggagcaacc ttactggatg
    28741 ctcaacaacg ggtattgcgc cctgccattt tgtggaacga cgggcgctgt gcgcaagagt
    28801 gcactttgct ggaagcgaga gttccgcaat cacgagtgat taccggcaac ctgatgatgc
    28861 ccggatttac tgcgcctaaa ttgctatggg ttcagcggca tgagccggag atattccgtc
    28921 aaatcgacaa agtattatta ccgaaagatt acttgcgtct gcgtatgacg ggggagtttg
    28981 ccagcgatat gtctgacgca gctggcacca tgtggctgga tgtcgcaaag cgtgactgga
    29041 gtgacgtcat gctgcaggct tgcgacttat ctcgtgacca gatgcccgca ttatacgaag
    29101 gcagcgaaat tactggtgct ttgttacctg aagttgcgaa agcgtggggt atggcgacgg
    29161 tgccagttgt cgcaggcggt ggcgacaatg cagctggtgc agttggtgtg ggaatggttg
    29221 atgctaatca ggcaatgtta tcgctgggga cgtcgggggt ctattttgct gtcagcgaag
    29281 ggttcttaag caagccagaa agcgctgtac acagcttttg ccatgcgcta ccgcagcgtt
    29341 ggcatttaat gtctgtaatg ctgagtgcag catcgtgtct ggattgggtc gcgaaattaa
    29401 ccggcctgtg caatgtccca gctttaatcg ctgcagctca acaggctgat gaaagtgccg
    29461 agccagtttg gtttctgcct tatctttccg gcgagcgtac accacacaat aatccccagg
    29521 cgaagggggt tttctttggt ttgactcatc aacatggccc caatgaactg gcgcgagcag
    29581 tgctggaagg cgtgggttat gcgctggcag atggcatgga tgtcgtgcat gcctgcggca
    29641 ttaaaccgca aagtgttacg ttgattggtg gcggggcgcg tagtgagtac tggcgtcaga
    29701 tgctggcgga tatcagcggt cagcagctcg attaccgtac ggggggggat gtggggccag
    29761 cactgggcgc agcaaggctg gcgcagattg cggcgaatcc agagaaatcg ctcattgagt
    29821 tgttgccgca gctaccgtta gaacagtcgc atctaccaga tgcgcagcgt tatgccgctt
    29881 atcagccacg acgtgaaacg ttccgtcgcc tctatcagca acttctgcca ttaatggcgt
    29941 aaacgttatc ccctgcctga ccgggtgggg gataattcac atctatatat ctcactaatt
    30001 aattaatatt tagtatgaat ttattctgaa aatcatttgt taatggcatt tttcagtttt
    30061 gtctttcgtt ggttactcgt aatgtatcgc tggtagatat ggagatcgtt atgaaaacct
    30121 caaaaactgt ggcaaaacta ttatttgttg tcggggcgct ggtttatctg gttgggctat
    30181 ggatctcatg cccattgcta agtggaaaag gctattttct tggcgtgtta atgacagcaa
    30241 cttttggtaa ctatgcatat cttcgcgcag aaaaactcgg gcaactggat aattttttta
    30301 cccatatctg ccagttagtt gcgttaatca ctatcggtct cttgtttatc ggtgttttaa
    30361 atgcacctat caatgcttat gaaatggtga tctaccccat cgcctttttt gtctgcttgt
    30421 ttggtcaaat gcgtttgttt cgctcggtat gagcaacata aagctcttac acattcagga
    30481 atgaaaggaa tactgtgatg gacaacaaaa tatcaaccta ttcaccggcc tttagtattg
    30541 tgtcatggat agctctcgtt ggtggtatcg ttacctatct gttagggcta tggaatgcag
    30601 agatgcagtt aaatgaaaaa gggtattatt ttgccgtact ggtgttagga ctgttttctg
    30661 ctgcgtctta tcaaaagacc gttcgggaca agtatgaagg cataccgacc acttccattt
    30721 attatatgac ctgcctgact gtctttatta tctctgttgc attactgatg gtaggtctgt
    30781 ggaatgcgac attattactc agtgaaaaag gtttttatgg actggctttc ttcttaagct
    30841 tgtttggtgc agtagcggtg cagaagaata ttcgtgatac cggaataaac ccgccaaaag
    30901 aaacacaggt tacccaggaa gaatacagcg aataattcac gtaagcccgg tcagtccaat
    30961 gtgaacgggc ttttacttaa ctcactaatc tgtttctgtc gattcgttgt accagcatag
    31021 aaagtaacaa actcgctgcc aacgtcgcgc aaaagatcca aataatatcc agtattggcc
    31081 aatttttaag ctcaattccc cgggtgcgca gcgcatggat aatcaaggcg tggaatccgt
    31141 atatacccaa tgaatggcgg gagattaagc caagtccgcg aatggtacgc gtatccagcg
    31201 tgtttttaac cagagtcaat agcgcgattg cgcagataaa aaccatcggc ccacagtaaa
    31261 gataccaggt atcggcaaaa tttccgcgcc actgcaattc atataatgtc ccgcgagaga
    31321 taataaaaac ccccgtcgca aacagcgcgg cgctcaccca cgacagtgct ttatgctgtg
    31381 tgtccatcat ccctatagcg cggcccaaca tgccatacag aatgtagtaa aaagtatcgc
    31441 cattgatata taagttaatt ggcagccatt caaaaccgtc aattttctgc ggcactgtgt
    31501 ttgggttagc gataatgcca atcaccacca ttagcaccag caacattttt ccgccgacgt
    31561 tcttcacctg aatcagcggt gaaaccagat aaatcaccgc aatcgcgaag aaaaaccaca
    31621 agtggtaaaa cactggcttt tgcagcaggt ttttcagcgc taactccata ttgatggagg
    31681 taaacagcgc aatgtagagc agtgcgattg cgctataaaa aatcagacat aagccgatac
    31741 gcaagaaatg gcgcggctgg gcgctgcgtt cgccaaaaaa gagatagccg gaaatcatga
    31801 aaaatagcgg cacgctgaca cgagaggcag aattcagaac attggcgata tcccatgtga
    31861 cggggctaac actatgagca ttggtcacat accaggtagt ggtgtgaatc atcaccacca
    31921 ttaaacacgc tatccctcgc aggttatcaa tccagtaaat tttgggctgc atctgtgtct
    31981 ctgtatctgg ttaaaaaaag tctgaccgat aaatcatttg gttggcgcac tggaataatc
    32041 tgagttttat cactacagct tatagaggct taaggaaatt cgtaagatat cagccactat
    32101 accgatataa ataataagac tcacctgcaa accagacggt aatttaatga tgatgaacgc
    32161 tttctttccg gcaatggcgc ttatggtgct agtgggttgt tctacaccgt cacccgtgca
    32221 gaaagcacaa cgggtaaagg ttgatcctct gcgttcgttg agtatggaag cgttatgcaa
    32281 ggatcaggcg gcaaaacgtt ataacaccgg cgagcaaaaa atcgacgtca ccgccttcga
    32341 acagttccag ggaagctatg aaatgcgcgg ttataccttc cgtaaagagc agtttgtctg
    32401 ttcttttgac gcagatggcc attttttgca tctttccatg cgttaagccc tgctttttcc
    32461 cgtttcgtac tgtatatctt ccatccagcg ggtatactga tcccttcctt taaatccaca
    32521 cgtatccagc acgaaataat atgcaaaagt ttgataccag gaccttccag ggcttgatcc
    32581 tgaccttaca ggattactgg gctcgccagg gctgcaccat tgttcaacca ttggacatgg
    32641 aagtcggcgc gggaacctct cacccaatga cctgtctgcg cgcgctgggg ccagaaccga
    32701 tggcggctgc ttatgttcag ccttctcgtc gcccgaccga tggtcgctac ggcgaaaacc
    32761 ccaaccgttt acagcactac tatcagttcc aggtggtcat taagccatcg ccggacaata
    32821 ttcaggagct gtacctcggt tctctgaaag agctgggcat ggacccgact attcacgaca
    32881 tccgtttcgt ggaagataac tgggaaaacc cgactctggg tgcctgggga ctgggctggg
    32941 aagtgtggct aaacggcatg gaagtgacgc agttcactta cttccagcag gttggtggtc
    33001 tggagtgtaa accggttacc ggcgagatca cctacggtct ggaacgtctg gccatgtaca
    33061 ttcagggcgt agacagcgtt tacgacctgg tctggagcga cggcccgctg ggtaaaacca
    33121 cctacggcga cgtgttccat cagaacgaag tggagcagtc cacttacaac ttcgaatacg
    33181 cggatgtgga cttcctgttc acctgctttg agcagtacga gaaagaagcg cagcagctgc
    33241 tggcgctgga aaatccgctg ccgctgccag cctacgagcg tattctgaaa gccgcccaca
    33301 gcttcaacct gctggatgcg cgtaaagcca tctccgtcac cgagcgtcag cgctacattc
    33361 tgcgcattcg caccctgacc aaagcagtgg cagaagcata ctacgcttcc cgtgaagccc
    33421 tcggcttccc gatgtgcaac aaagataagt aagaggcggc tatgtctgag aaaacttttc
    33481 tggtggaaat cggcactgaa gagctgccac caaaagcact gcgcagcctg gctgagtcct
    33541 ttgctgcgaa ttttactgcg gagctggata acgctggcct cgcacacggc accgttcaat
    33601 ggtttgctgc tccgcgtcgt ctggcgctga aagtagctaa cctggcggaa gcgcaaccgg
    33661 atcgtgaaat cgaaaaacgc ggcccggcga ttgcccaggc gttcgacgct gaaggcaaac
    33721 cgagcaaagc ggcagaaggt tgggcgcgtg gttgcggtat taccgttgac caggctgagc
    33781 gtctgactac cgataaaggc gaatggctgc tgtatcgcgc ccatgtgaag ggcgaaagca
    33841 ccgaagcact gctgccgaat atggttgcga cttctctggc gaaactgccg atcccgaaac
    33901 tgatgcgttg gggcgcaagc gacgtgcact tcgtgcgtcc ggtgcacacc gtgaccctgc
    33961 tgctgggcga caaagtcatt ccggcaacca ttctgggcat tcagtccgat cgcgtgattc
    34021 gcggccaccg ctttatgggc gaaccggaat tcaccatcga caacgccgat cagtatccgg
    34081 aaattctgcg tgagcgcggg aaagtcatcg ccgattacga agaacgtaaa gcgaagatta
    34141 aagccgatgc cgaagaagca gcgcgtaaga ttggcggtaa cgctgactta agcgaaagcc
    34201 tgctggaaga agtggcttcg ctggtggagt ggccggtcgt tctgaccgca aaattcgaag
    34261 agaaattcct cgcggtgccg gctgaagccc tggtttacac catgaaaggt gaccagaaat
    34321 acttcccggt gtatgcgaac gatggcaaac tgctgccgaa cttcatcttc gttgccaaca
    34381 tcgaatcgaa agatccgcag caaattatct ctggtaacga gaaagtcgtt cgtccacgtc
    34441 tggcggatgc cgagttcttc ttcaataccg accgtaaaaa acgtctggaa gataacctgc
    34501 cgcgcctgca aaccgtgctg ttccagcaac agctgggtac gctgcgcgac aaaactgacc
    34561 gcatccaggc gctggctggc tggattgctg aacagattgg cgctgacgtt aaccacgcaa
    34621 cccgtgcggg cctgctgtct aagtgtgacc tgatgaccaa catggtcttc gagttcaccg
    34681 acacccaggg cgttatgggg atgcactatg cgcgtcacga tggcgaagcg gaagatgtcg
    34741 cggtggcgct gaatgagcag tatcagccgc gctttgccgg tgatgacctg ccgtctaacc
    34801 cggtagcctg tgcgctggcg attgctgaca agatggatac tctggcgggt atcttcggta
    34861 tcggccagca tccgaaaggc gacaaagacc cgtttgcgct gcgtcgtgcc gcacttggcg
    34921 tgctgcgaat tatcgttgag aagaacctca accttgatct gcaaacgctg accgaagaag
    34981 cagtgcgtct gtatggcgat aagctgacta acgccaacgt ggttgatgat gttatcgact
    35041 ttatgctcgg tcgcttccgc gcctggtatc aggacgaagg ttacaccgtt gacaccatcc
    35101 aggcggtact ggcgcgtcgt ccgactcgtc cggctgattt cgatgcccgc atgaaagcgg
    35161 tatcgcactt ccgtaccctt gaagcagctg ctgcactggc ggcggcgaac aagcgtgtct
    35221 ccaacatttt ggcgaaatct gacgaagtgc tgagcgaccg cgtgaatgcc tctaccctga
    35281 aagagccgga agaaattaaa ctggcgatgc aggttgtggt gctacgtgac aagctggagc
    35341 cgtactttgc ggaaggtcgt taccaggatg cgctggtcga actggcagag ctgcgtgagc
    35401 cggttgatgc tttcttcgat aaagtgatgg tcatggttga tgacaaagaa ttgcgtctca
    35461 accgtctgac catgctggag aaactgcgcg aactgttcct gcgcgttgcg gatatttcgc
    35521 tgttgcagta ataacgccgt tattaaaaag cctgccatct ggcaggcttt ttttattatc
    35581 gctaaataat acagcaacct ttaataatct tctgctgaat aaagattatc tcatatatta
    35641 attttatgag gttttttagg attatatcaa ggagaagaaa caaacttatt aagctagaat
    35701 agccacgggt gcttgagact gtttgtctca ggtattcacc gaaaggcaga cagagaaaag
    35761 ccccacctga ctataaatca aagtgaggct accctatgcc tgaacaccat aaggttagcc
    35821 tcttactcgt tggaaatcaa cacagggggc tgggaatgcc gcagaaatat agattacttt
    35881 ctttaatagt gatttgtttc acgcttttat ttttcacctg gatgataaga gattcactgt
    35941 gtgaattgca tattaaacag gggagttatg agctggcggc atttttagcc tgcaatttaa
    36001 aagagtaaga gtcttcggcg ggaagttatt cccgcctttc ttacggcgtt gcgcattctc
    36061 attgcaccca aatttatttt tcacaaaaat aatactagat tttattacgc gatcgattat
    36121 ttatttcctg aaaaacaaat agaaaaatcc ccgccaaatg gcagggatct tagattctgt
    36181 gcttttaagc agagattaca ggctggttac gttaccagct gccgggcctt tagcgccgct
    36241 ttcgatggtg aaggacactt tctgaccttc gtccagagat ttgtaaccat cgttctggat
    36301 agcagagaag tgtacgaaca cgtctttaga gccatcgtca ggagtgatga agccgaagcc
    36361 tttgtcagcg ttgaaccatt ttacgatacc agtcatttta ccggacatag tgtattacct
    36421 ttaataatta agtgtgcctt tcggcgatat ggcgtgcttt acagattttg aagcgttaaa
    36481 ggaatgtgca ctacgagggg tatcaacgat aactcttgaa gggacttgcc ttactacact
    36541 gctttaatgg tctgtacgtc aaaccgttga tgtgcattaa gccacgcatt ggcgggtgat
    36601 gcaacaatta tttttcatat ttatgattaa tcggttggaa aacggtcctg tcatcaggac
    36661 cgtaaacagc aataaagtgg ataaaagtct attccatcaa ctgcttactt aatgccgggt
    36721 tggcttgaat caaacgcatc aattttagtt cggcacttga aggcttcacg cgtctggatt
    36781 cccattcctt taccatggcg actgatacgc ccaaaacccg ggcgaaatca tcgattttta
    36841 accctgtccc ttttcgtaac tgctcaattt cggtacagga cgttgttctg tgcgtcaggg
    36901 taattttctg cgtttcatct ttaaaaacaa tctgttccag gctgctcaac agctcatgca
    36961 ttggatcttt atattccatt gaaaactcct caaatcacac tgcggggtcg tgaattgcat
    37021 cgaagctcat taagaatagt cgggaaaagc agacacagcg gggtgaagtc agggcatgct
    37081 ctggctatga ttcgattcag ccagtcattt agcgtgactt atagccacct aacgatctga
    37141 taaatgccga tatatctctt accactcctg gtttgtaaag atttcgaaaa tctaaccttc
    37201 tgcgaaggcg taaatctctg gctacaaaaa aagcaacggc actatcattt tttaacaatg
    37261 aaacaacaaa ttaaccttgt ttcattttct ccatgatttt accaggaaga ttacgtagaa
    37321 agggtatcct gcaagcctgt cctggactat ccttgtcacg tcagacacgc gtgtgtcgtt
    37381 gtgcgctttt tttgggtgaa aggagtaaga aaatggcgac aggaaagtcc tgctctcgct
    37441 ggtttgcgcc tcttgcggcg ttattaatgg tagttagcct gagtgggtgt tttgataaag
    37501 aaggcgatca gcgtaaagcg tttatcgact tcctgcagaa tacagtgatg cgtagcggtg
    37561 aacgtctacc aaccctgact gccgatcaga aaaaacagtt tggtcctttt gtctctgatt
    37621 acgcgattct gtatggttat tctcagcagg tgaatcaggc gatggattcc ggtctgcgcc
    37681 cggtcgtaga cagcgttaac gcaattcgcg taccgcagga ttacgttacg caaagcggtc
    37741 cgctgcgtga aatgaacggt tcgctgggtg tgctggcgca acaactgcaa aatgcgaagt
    37801 tgcaggccga tgctgcgcac tctgcgctaa aacagaccga tgatttgaaa ccggtctttg
    37861 atcaggcatt caccaaagtg gtaacgacgc cagctgatgc attgcaaccg ttgattccgg
    37921 ctgcacaaac tttcacgcaa caactggtga tggttggaga ctacattgct cagcaaggta
    37981 ctcaggtgag ctttgtcgca aatggcatcc agttcccgac ttcacagcag gcaagtgaat
    38041 ataacaaact gattgcgcca ttaccggcac aacatcaggc gtttaatcag gcctggacta
    38101 cagcagttac tgcaacccaa taaagagtaa aagcccgagc gattatcggg ctttacttca
    38161 cgcagtcgcg gcttagtccg cgacgtgcgg attcacacag ttcttctcaa cctttccttg
    38221 taacgcatca atcaaattat ccacggcaca ggcggccatg ccataacgcg tctcatgggt
    38281 ggcagatcca atatgcggta ctgcgacgac gttggccatt gagagcaacg gcgaatctac
    38341 ggacagtggc tcttgttcga agacatccag cccggcggcg tgaatttcgc ctttctgcaa
    38401 tgcagcaatc agtgcatttt catcaaccac cgggccacgt ccggcattaa tgaaaatggc
    38461 ggaggatttc attttggcga attgttctgc gccaaacaga tgatgcgtct catcagttaa
    38521 cggcaggatc aggcaaacga aatctgactc ttgtaacaga gtatccaaat cgcagtagcg
    38581 ggcgttgaag cgttcttctg cttctttatg gtggcggcgc gcgttataga ggatgggcat
    38641 gttgaagcca aagtgcgcac gttgtgccag cgccataccg atccgtccca tcccgacaat
    38701 gcccagtgtt ttatggtgaa cgtcagtgcc gtaccagtcc gggcctatgc tcgcggtcca
    38761 ttcgcctgct tttacccgtt ctgctacctc cacaacccga cgagcggtag acaacaccag
    38821 cgccatcagc gtatcggcga cggtttctgt taatacggtt ggcgtgtgca tcagcagaat
    38881 ttttcgggcg gtaagcgcat cgacatcaaa attgtcatag ccgacggaga tcgttgatgt
    38941 ggcacgcagt ttcggcattt tttccagcaa tgcggcatca acattctcgt ttgaacccag
    39001 taaaccttca gcttcggcaa aaattgctgc attttgttcg acggtttgtg ggctgaggtt
    39061 tgccacctgg tgaacggtga aatgctcttg caggcgttgc agtaaatcat caggtaaggc
    39121 tttgtagagg ataacggacg gcttcatgct tctctccatt cactgataag gcgatcacct
    39181 tagcctgaaa tatgatgaag gaaaactgaa gaatgccagg tgatttctgc atcacctggc
    39241 atgaaaggga ttacagcggg cttaaggtaa tttctacacg gcggttttgc gccttacctt
    39301 ctgcggtgct gttgctggcg attgggttag ccgggccaag gccctgagta cggatgcggc
    39361 tggcgtccac gccctgggtg atcaacgcgc tggcaacgga atccgcacgt tgctgggaga
    39421 gacgcatgtt caggtcgtga ccacccgtgc tgtcggtata accaatcacg ttaaccgccg
    39481 ttttcggata ctctttcagc accattgcca cgccggtcag ggtgttagcg cccgcaggtt
    39541 tcagggtcgc gctgctgctg tcgaaggtca cattgttcgg catattgagg ataatgttat
    39601 ccccgctgcg ggttacgcta acaccagtgc cgcgcatttt gtcacgcagc ttcgcttcct
    39661 gcacatccat gtaataaccc acgccgccgc ccagagctgc gcctgctgct gcgccaatca
    39721 gcgcgccttt accgcgatct ttcttcgaag aagagagcgc accaataccc gcgcccacga
    39781 gagagcccag acctgcgccg atagcagatt tacctgcttc gcgttcgccg gtgtaagggt
    39841 tagttgtgca gccagatacc gccagagcgc cactcactac ggcggcaata agataaacac
    39901 gtttcttcat tgttaatcct taataacctt tttattcttt gccacgggtt ccgtggcggg
    39961 agattatgcc gcgtgaacat gaagattatt cctgggaata ctcggaaatt tgtaagtaat
    40021 atttaactgc tcaatacatc taacctttca ggagttttcg gtttggccaa ctcatcctca
    40081 cgatattccg ttctgactgc cgcccattgg gggcccatgc tggttgaaac cgacggcgaa
    40141 accgtgttta gctcgcatgg cgcgttagcc acaggaatgg aaaactcctt gcagagcgcg
    40201 gttcgcgacc aggttcacag caatacacgg gtacgatttc caatggtgcg caaaggcttt
    40261 cttgcgtcac cggaaaatcc gcaaggcatt cgtgggcagg atgaatttgt tcgcgtgagt
    40321 tgggatgagg cgctggatct tattcaccaa caacataaac gcattcgtga ggcttatggt
    40381 ccggcatcga tttttgctgg ttcctacggc tggcgttcaa acggcgtgct gcataaggcc
    40441 tcgacattat tacaacgcta tatggcgctg gcaggcggtt ataccgggca tctgggggat
    40501 tattcgaccg gcgcggcaca ggcgatcatg ccgtatgtcg tgggtggtag cgaagtttat
    40561 caacagcaaa ccagttggcc gctggtgctg gaacatagcg atgtcgtggt gttgtggagt
    40621 gctaacccac tcaatacgct gaaaattgcg tggaatgcat ctgatgagca ggggctttct
    40681 tacttttccg cactgcgtga cagcgggaaa aagctgatct gtattgatcc aatgcgatcg
    40741 gaaaccgtcg atttctttgg cgataaaatg gagtgggtgg caccgcacat gggcaccgat
    40801 gttgcgctga tgctggggat cgcccatacg ctggtggaaa atggttggca cgacgaagcg
    40861 tttctgacgc gttgcaccac aggttatgcc gtcttcgcct cttatttgct gggcgagagt
    40921 gacggaatag cgaaaaacgc cgaatgggcg gcagagattt gtggggttaa cgcagcgaaa
    40981 atccgcgagc tggcggctct tttccaccaa aacacgacca tgctgatggc tggctgggga
    41041 atgcagcgcc aacagtttgg tgagcaaaaa cactggatga tcgtcacgct ggcggcaatg
    41101 ttggggcaaa tcggcacacc cggcggcggt tttggtcttt cttatcattt tgccaatggt
    41161 ggtaacccca cgcggcgttc tgcggtgctc tcttccatgc agggcagctt gccgggtggc
    41221 tgcgatgcgg tggataaaat ccctgttgcc cgcattgttg aagcactgga aaaccctggt
    41281 ggcgcatatc aacacaacgg tatgaaccga catttcccgg atattcgttt tatctggtgg
    41341 gcgggcggtg ccaactttac tcatcatcag gataccaatc gtctgatccg tgcctggcaa
    41401 aaaccggagc tggtggtgat ctctgaatgc ttctggacgg cggcggcgaa acacgcggat
    41461 atcgttctgc ctgcgactac ctcttttgag cgtaatgatc tcaccatgac cggtgattac
    41521 agtaatcagc atctggtgcc gatgaagcaa gtggtgccgc cacgctatga agcgcgtaat
    41581 gattttgatg tttttgccga gttgagtgaa cgttgggaga agggcggcta tgcgcggttt
    41641 acggaaggaa aaagtcagct gcaatggctg gaaacgtttt ataacgttgc ccggcagcgc
    41701 ggggcaagcc agcaggttga attgccgcca tttgctgagt tctggcaagc caaccagtta
    41761 attgagatgc cggaaaaccc ggacagcgag cggtttattc gcttcgctga tttttgccgc
    41821 gatccgctgg cgcatccgtt aaaaaccgcc agcggcaaga ttgaaatctt ctcacagcgt
    41881 attgccgatt acggttaccc ggattgccct gggcatccaa tgtggctgga gccggacgaa
    41941 tggcagggca atgccgaacc agaacagttg caggtacttt ctgcccatcc ggcgcaccgc
    42001 ctgcacagcc agctgaatta cagttctctg cgcgaattgt acgcggtagc aaaccgtgag
    42061 cctgtcacca ttcatcctga cgatgctcag gcgcgcggca taaccgaagg ggatatggtg
    42121 cgggtgtgga actcccgtgg gcagatcctt gccggagcgg tcattagcga gggaattaaa
    42181 cctggcgtga tttgcattca cgaaggggca tggccggatc tggatttaac cgctgacggt
    42241 atttgtaaaa acggcgcagt gaacgtgctg accaaagatc tccccagctc gcggctgggg
    42301 aatggctgtg cgggtaatac ggcgctggca tggctggaaa aatacaacgg tccggaactg
    42361 acacttacag cgtttgaacc accggccagc tcataatcca ggtgggtagt tgggtttcat
    42421 cctgccatgc gcaatcgaca atgtgaaaac cctgcgcacg gtaaaaatct atcgccggtt
    42481 gatttttttg ataaacctcc agcatcagat ggggatagcg ctgctgcaca tactgcatca
    42541 gcgccttacc aataccgcgt ctgacggcct tcggtgcgac aaacattgct gccagaaatc
    42601 ggccttccat aatgctgaca aaaccgagaa gcttaccgtc ctcttcccag acccagtttt
    42661 gcgcgttggc aagataggca tcccgcacca gcggaatgca gtcacgccag taattcgctt
    42721 ttataaaggg atgcccccag gttgtacttt ccagccacag ttcgaggatc gcggggagtt
    42781 ctgaacgttg cgcttcccga atcatggttt atttcccgga tagcaacagc agccaaccac
    42841 atgatcattc accagcccac atgcctgcat aaaggagtaa cagattgtgg tgccgacaaa
    42901 cttaaaacca cgttttttca gtgccttaga tagggcgtcg gaggcggacg tagatgtggg
    42961 aatttcgctc aacgttgtgg cttgtgtcac ctgtggctga tgatttacaa acgaccagac
    43021 aaagtcggca aacggttcgc cgttctgttc catttgcagg tacgcccgcg cattaccaat
    43081 aattgcctga attttccctc gatggcggat aatcccggcg tcctgtacca gtctttcgac
    43141 atcctcttcc tgcattgctg cgaccttcac cggatcgaac tgatgaaagc aggcgcgata
    43201 gttttcgcgt tttttgagga cggtgatcca cgataaccca gcctgctgcc cttcaaggca
    43261 gatcatttcg aacagttttt tactgtcagt ttcaggcacg ccccattcat tatcatggta
    43321 ggcaatataa agcgggtcct gactcaccca gccgcaacgt tccatacttt ccctcgctat
    43381 gtacccgatc gtaaaaattt cagccgcttt tcttgacgca tcggtgaaaa agtcaatatt
    43441 taatacaggg ctactatgcc cgatatcctt catgcaatga caataatatt gccgaattcg
    43501 gctcttccct ggagtcgtgt taatgatcat aaaaaaaagc ggtggtcgct ggcagctaag
    43561 cctgctggcg agcgtggtaa tcagtgcctt ttttctcaac acagcttacg cctggcaaca
    43621 agaatatatc gttgatacgc aacccggaca ttccacagag cgttacacct gggatagtga
    43681 tcatcaacct gattacaacg atattttgtc gcaacgtatt caaagtagcc aaagggcgct
    43741 gggactggaa gtcaatctgg cggaagaaac tcctgtggat gtgaccagca gtatgagtat
    43801 gggctggaat tttcctttat atgaacaggt tacaaccggc ccggtcgcgg cattacatta
    43861 cgatggcaca accacctcga tgtataacga gtttggcgac agtactacca cgctgaccga
    43921 tccgttatgg catgccagcg tgagtagctt aggctggcgt gttgactccc ggcttggcga
    43981 tctccgaccc tgggcgcaaa tcagctataa ccagcaattt ggcgagaata tctggaaggc
    44041 gcaatcaggc ctgagccgga tgacggcgac aaaccagaac ggcaactggc tggatgtcac
    44101 cgtaggcgct gatatgttgc tcaatcaaaa tattgccgcc tatgccgcgc taactcaggc
    44161 agaaaatacc actaataata gcgactatct gtatacgatg ggggttagcg ccagatttta
    44221 acgtaacagt cacaattgaa accattgaat aacaacagtt gtggcgatag tgggtgctaa
    44281 cttaccaaat aataaatttg gtgaataatt gtcgcgtcat tcattcctga actaaggcat
    44341 ttcattccgt tctgatggca tttcatgccg tttttcccca ggcataaaat gcacttcgtt
    44401 atggttgtcg gcagagattt ttccttttta ttactgcagg aatactgcca tgacaccttc
    44461 aaattatcag cgtacccgct ggctgacact catcggtact atcattaccc agtttgcact
    44521 ggggtcggtt tatacctgga gcctgtttaa tggcgcgctt tctgccaagc tggatgcgcc
    44581 ggtaagccag gtcgctttct ctttcggctt gttaagtctg gggctggcaa tttcgtcttc
    44641 tgttgcgggc aaattgcagg aacgttttgg cgttaaacgc gtcaccatgg cttccggcat
    44701 tttgctggga ttaggcttct tcctgaccgc gcattccaac aacctgatga tgctgtggtt
    44761 aagcgccggt gtgctggtcg gtctggcgga tggcgcgggt tacctgctga cgctctctaa
    44821 ctgcgtgaag tggttcccgg agcgtaaagg tctgatatcc gcgttcgcta tcggttctta
    44881 tggtctgggt agcctgggtt tcaaatttat cgacacgcag ctgctggaaa cggtgggtct
    44941 ggaaaaaacc tttgtgattt ggggagcgat tgcgctggtg atgattgttt tcggcgcaac
    45001 gttaatgaaa gacgcaccaa aacaggaagt gaaaaccagc aatggtgtgg tggagaaaga
    45061 ttacacgctg gcagagtcga tgcgtaaacc gcagtactgg atgttagcgg taatgttcct
    45121 gaccgcctgc atgagcggcc tgtacgtgat tggggtagcg aaagatatcg cccaaagtct
    45181 ggcacacctt gatgtggttt ccgcagccaa tgcagtcact gttatttcca tcgccaacct
    45241 ttcaggtcgt ctggtgctgg gtattctgtc tgacaaaatc gcccgtatcc gtgttatcac
    45301 cattggtcag gtgatatcgc tggtgggtat ggcggccctg ctgtttgcac cattgaatgc
    45361 agtgacgttc tttgcagcga ttgcctgcgt ggcatttaac tttggcggca ctattaccgt
    45421 ctttccgtca ctggtcagtg agttctttgg cctcaataac ctggcgaaaa actacggtgt
    45481 gatttacctc ggtttcggta tcggtagcat ttgtggttcg attatcgcct cactgtttgg
    45541 cggcttctat gtgaccttct acgtgatttt cgccctgctg attctgtcat tggcgctttc
    45601 tacgacaatt cgtcagccag agcagaaagt attgcgtgag gcgcatattt agaaatatgt
    45661 tgctactgaa ataaatgcga ggagggagac ttcaaagtct cccttttttt atggacgcat
    45721 atta
//
LOCUS       DAZGOB010000025        44635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGOB010000025 DAZGOB010000000
VERSION     DAZGOB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.025"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(187..1551)
                     /gene="mnmE"
                     /locus_tag="ACHLIF_001447"
     CDS             complement(187..1551)
                     /gene="mnmE"
                     /locus_tag="ACHLIF_001447"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO9605486.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLYDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1657..3303)
                     /gene="yidC"
                     /locus_tag="ACHLIF_001448"
     CDS             complement(1657..3303)
                     /gene="yidC"
                     /locus_tag="ACHLIF_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO9605487.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3306..3563)
                     /gene="yidD"
                     /locus_tag="ACHLIF_001449"
     CDS             complement(3306..3563)
                     /gene="yidD"
                     /locus_tag="ACHLIF_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO9605488.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3527..3886)
                     /gene="rnpA"
                     /locus_tag="ACHLIF_001450"
     CDS             complement(3527..3886)
                     /gene="rnpA"
                     /locus_tag="ACHLIF_001450"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO9605489.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3903..4043)
                     /gene="rpmH"
                     /locus_tag="ACHLIF_001451"
     CDS             complement(3903..4043)
                     /gene="rpmH"
                     /locus_tag="ACHLIF_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO9605490.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4273..4353)
                     /locus_tag="ACHLIF_001452"
     CDS             complement(4273..4353)
                     /locus_tag="ACHLIF_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605491.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4650..6053
                     /gene="dnaA"
                     /locus_tag="ACHLIF_001453"
     CDS             4650..6053
                     /gene="dnaA"
                     /locus_tag="ACHLIF_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO9605492.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNIPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6058..7158
                     /gene="dnaN"
                     /locus_tag="ACHLIF_001454"
     CDS             6058..7158
                     /gene="dnaN"
                     /locus_tag="ACHLIF_001454"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO9605493.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7158..8231
                     /gene="recF"
                     /locus_tag="ACHLIF_001455"
     CDS             7158..8231
                     /gene="recF"
                     /locus_tag="ACHLIF_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO9605494.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8260..10674
                     /gene="gyrB"
                     /locus_tag="ACHLIF_001456"
     CDS             8260..10674
                     /gene="gyrB"
                     /locus_tag="ACHLIF_001456"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO9605495.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10914..11312
                     /gene="yidB"
                     /locus_tag="ACHLIF_001457"
     CDS             10914..11312
                     /gene="yidB"
                     /locus_tag="ACHLIF_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO9605496.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11427..12239
                     /gene="yidA"
                     /locus_tag="ACHLIF_001458"
     CDS             11427..12239
                     /gene="yidA"
                     /locus_tag="ACHLIF_001458"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO9605497.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12285..12941)
                     /gene="yidX"
                     /locus_tag="ACHLIF_001459"
     CDS             complement(12285..12941)
                     /gene="yidX"
                     /locus_tag="ACHLIF_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO9605498.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYHWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13219..13908
                     /gene="dgoR"
                     /locus_tag="ACHLIF_001460"
     CDS             13219..13908
                     /gene="dgoR"
                     /locus_tag="ACHLIF_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO9605499.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13905..14783
                     /gene="dgoK"
                     /locus_tag="ACHLIF_001461"
     CDS             13905..14783
                     /gene="dgoK"
                     /locus_tag="ACHLIF_001461"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO9605500.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14767..15384
                     /gene="dgoA"
                     /locus_tag="ACHLIF_001462"
     CDS             14767..15384
                     /gene="dgoA"
                     /locus_tag="ACHLIF_001462"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO9605501.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15381..16529
                     /gene="dgoD"
                     /locus_tag="ACHLIF_001463"
     CDS             15381..16529
                     /gene="dgoD"
                     /locus_tag="ACHLIF_001463"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO9605502.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16604..17941
                     /gene="dgoT"
                     /locus_tag="ACHLIF_001464"
     CDS             16604..17941
                     /gene="dgoT"
                     /locus_tag="ACHLIF_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9605503.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17938..19002)
                     /gene="cbrA"
                     /locus_tag="ACHLIF_001465"
     CDS             complement(17938..19002)
                     /gene="cbrA"
                     /locus_tag="ACHLIF_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO9605504.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEIYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKRIMRSGMAHIPQLKD"
     gene            19103..20317
                     /gene="yidR"
                     /locus_tag="ACHLIF_001466"
     CDS             19103..20317
                     /gene="yidR"
                     /locus_tag="ACHLIF_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO9605505.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYMPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAEVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20319..20651)
                     /gene="yidQ"
                     /locus_tag="ACHLIF_001467"
     CDS             complement(20319..20651)
                     /gene="yidQ"
                     /locus_tag="ACHLIF_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO9605506.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20891..20963
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20957..21370
                     /gene="ibpA"
                     /locus_tag="ACHLIF_001468"
     CDS             20957..21370
                     /gene="ibpA"
                     /locus_tag="ACHLIF_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO9605507.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21482..21910
                     /gene="ibpB"
                     /locus_tag="ACHLIF_001469"
     CDS             21482..21910
                     /gene="ibpB"
                     /locus_tag="ACHLIF_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO9605508.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            22107..23768
                     /gene="yidE"
                     /locus_tag="ACHLIF_001470"
     CDS             22107..23768
                     /gene="yidE"
                     /locus_tag="ACHLIF_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO9605509.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDMPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23765..24481)
                     /gene="yidP"
                     /locus_tag="ACHLIF_001471"
     CDS             complement(23765..24481)
                     /gene="yidP"
                     /locus_tag="ACHLIF_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9605510.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24777..26393
                     /locus_tag="ACHLIF_001472"
     CDS             24777..26393
                     /locus_tag="ACHLIF_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO9605511.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26393..27207
                     /locus_tag="ACHLIF_001473"
                     /pseudo
     CDS             26393..27207
                     /locus_tag="ACHLIF_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27204..28097)
                     /gene="yidL"
                     /locus_tag="ACHLIF_001474"
     CDS             complement(27204..28097)
                     /gene="yidL"
                     /locus_tag="ACHLIF_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9605512.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28264..29979
                     /gene="yidK"
                     /locus_tag="ACHLIF_001475"
     CDS             28264..29979
                     /gene="yidK"
                     /locus_tag="ACHLIF_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO9605513.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29976..31469
                     /gene="yidJ"
                     /locus_tag="ACHLIF_001476"
     CDS             29976..31469
                     /gene="yidJ"
                     /locus_tag="ACHLIF_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9605514.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDGLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31516..31965)
                     /gene="yidI"
                     /locus_tag="ACHLIF_001477"
     CDS             complement(31516..31965)
                     /gene="yidI"
                     /locus_tag="ACHLIF_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO9605515.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            32075..32422
                     /gene="yidH"
                     /locus_tag="ACHLIF_001478"
     CDS             32075..32422
                     /gene="yidH"
                     /locus_tag="ACHLIF_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO9605516.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32412..32774
                     /gene="yidG"
                     /locus_tag="ACHLIF_001479"
     CDS             32412..32774
                     /gene="yidG"
                     /locus_tag="ACHLIF_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO9605517.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32771..33268
                     /gene="yidF"
                     /locus_tag="ACHLIF_001480"
     CDS             32771..33268
                     /gene="yidF"
                     /locus_tag="ACHLIF_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO9605518.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33276..34460)
                     /gene="emrD"
                     /locus_tag="ACHLIF_001481"
     CDS             complement(33276..34460)
                     /gene="emrD"
                     /locus_tag="ACHLIF_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO9605519.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            34542..34616
                     /gene="ysdE"
                     /locus_tag="ACHLIF_001482"
     CDS             34542..34616
                     /gene="ysdE"
                     /locus_tag="ACHLIF_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO9605520.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(34879..34968)
                     /gene="tisB"
                     /locus_tag="ACHLIF_001483"
     CDS             complement(34879..34968)
                     /gene="tisB"
                     /locus_tag="ACHLIF_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO9605521.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35533..35631
                     /gene="ivbL"
                     /locus_tag="ACHLIF_001484"
     CDS             35533..35631
                     /gene="ivbL"
                     /locus_tag="ACHLIF_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO9605522.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35737..37425
                     /gene="ilvB"
                     /locus_tag="ACHLIF_001485"
     CDS             35737..37425
                     /gene="ilvB"
                     /locus_tag="ACHLIF_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO9605523.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDVFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37429..37719
                     /gene="ilvN"
                     /locus_tag="ACHLIF_001486"
     CDS             37429..37719
                     /gene="ilvN"
                     /locus_tag="ACHLIF_001486"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO9605524.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37794..38384
                     /gene="uhpA"
                     /locus_tag="ACHLIF_001487"
     CDS             37794..38384
                     /gene="uhpA"
                     /locus_tag="ACHLIF_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO9605525.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38384..39886
                     /gene="uhpB"
                     /locus_tag="ACHLIF_001488"
     CDS             38384..39886
                     /gene="uhpB"
                     /locus_tag="ACHLIF_001488"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO9605526.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39896..41215
                     /gene="uhpC"
                     /locus_tag="ACHLIF_001489"
     CDS             39896..41215
                     /gene="uhpC"
                     /locus_tag="ACHLIF_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO9605527.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            41471..42862
                     /gene="uhpT"
                     /locus_tag="ACHLIF_001490"
     CDS             41471..42862
                     /gene="uhpT"
                     /locus_tag="ACHLIF_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO9605528.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42907..>44635)
                     /gene="adeD"
                     /locus_tag="ACHLIF_001491"
     CDS             complement(42907..>44635)
                     /gene="adeD"
                     /locus_tag="ACHLIF_001491"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO9605529.1"
                     /translation="RAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGR
                     YIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTV
                     ICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLA
                     WRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGI
                     ENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPW
                     EIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLS
                     DARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPG
                     KRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGLN
                     EGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGL
                     MSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKF
                     AFTTLEVTE"
BASE COUNT        10290 a        11464 c        12125 g        10756 t
ORIGIN      
        1 tattctaaaa ttgtttaaat gtgaatcgaa tcacaatcgt tcggggagca atattaaatg
       61 ctttttttta agattataaa ttacgtaaga attttaatgt ttaaaaaaag ggttgaggaa
      121 taacaggagt gctaacaatg aagcgaagcc gcatctgaca gtcagaatac ggcttcgtaa
      181 gcgcggttac ttaccaatac agaagctgga gaaaatccgc cccagcaggt cgtctgaagt
      241 aaattccccg gtgatttcgc ttaagttctg ctgtgccagg cgcaactctt ccgccagcag
      301 ttcacccgcc caggcaccta acagttgcgc tttaccttgc tgcaaatgct ccgctgcctg
      361 ttccagcgcc tgtaagtggc gacggcgcgc caggaaaccg ccttccatgt tggtgtcaaa
      421 gcccatgctc tgtttgagat ggttacgcag cacgtccacg ccttcaccag tccttgccga
      481 gagacgaatt aacgcgtgac cgttcacttc actcattccc agcgtttcgc cggtgatatc
      541 ggctttattg cgcaccacgg tgatcggcag tttcgctggc agacgggcga taaattccgg
      601 ccagatctct gccggatcca cggcgtctgt tgtggtgcca tcgaccataa acagcacccg
      661 gtcggcctgt tcaatttcct gccacgcgcg ctcgatccca atacgttcca cttcgtcact
      721 ggcttcacgt agcccggcgg tgtcgatgat atgcagcggc attccgtcaa tgtggatatg
      781 ctcacgcaac acgtcacgcg tagttccggc gatatcggtt acgattgctg cttcacggcc
      841 agccagcgcg tttaacaggc tcgatttacc ggcgttagga cgtccggcaa tcaccacttt
      901 catcccttcg cgcaacaaac taccctgacg tgcttcagca cgcactgcat caagatcggc
      961 aataacgtca ttgagctggg cttcaatttt gccgtcggag aggaaatcga tctcttcatc
     1021 agggaaatca attgccgctt cgacgtaaat gcgcaagtgg gtgagggctt ctaccagatg
     1081 attaacccgt gcggagaatg cgccttgcag cgagttaagt gccgaacggg ccgcctgttc
     1141 cgagctggcg tcgataagat cggcaatcgc ctcagcctgg gctaagtcga gtttatcgta
     1201 aagaaacgcg cgttcggaaa actcaccagg gcgagcaatc cgcaggccgg gaatggtcag
     1261 aatgcgtttt aacagcaggt cgaggatcac cggaccgcca tgaccctgca gttcaagcac
     1321 atcttcgccg gtgaacgagt tcgggccagg gaaccatagc gcaatcccct gatcgagcac
     1381 gctgccgtcg gcgtctttaa atggcaaata atcggcgtag cgcggcttag gtagtttacc
     1441 cagcacggtt tcggcaactt cacgggcttt gaggccggag atgcgcagga tgccaacacc
     1501 gccacgtccc ggaggcgtgg cctgggctac gatagtgtca ttatcgctca tgatgttcct
     1561 gttgctttgt gtggcggatg cgcggtgctt atccgcccta cgaaaagaaa aaaggcggtc
     1621 aactgaccgc ccttatttta gcgaaaactc accgaatcag gattttttct tctcgcggct
     1681 atgcaggcca cgtttttcca gaccacggta aatcagctgc tgctgaataa tggttaccag
     1741 gttgctgacg atatagtaca gcaccagacc tgacgggaac cacaggaaga acacggtgaa
     1801 gatgaccggc ataaaggtca tgatcttctg ctgcatcggg tcggtcactg tggtcggcga
     1861 catcttctga atgaagaaca tcgttacgcc catcaggatc ggcaggatgt agtacgggtc
     1921 ctgtgccgac aggtcgtgga tccacagtgc aaacggtgcc tgacgcagtt caacggaacc
     1981 catcagcatg tagtacaacg ccaggaagat tggcatctgg atcagcagcg ggaagcagcc
     2041 gcccagcggg ttaaccttct cagctttgta cagcgccatc atttcctggc tgatacgctg
     2101 tttgtcatcg cccagacgct cacgcattgc ctgaatcttc ggctgcagca tacgcatctt
     2161 cgccatggag gtgtactgcg ctttggtcag cgggtacatg atgccacgaa cgataaaggt
     2221 gatgatgata atggagaagc cccagttacc cacaaagcta tggatccatt tcagcagttt
     2281 gaacagcggc tgagagatga accacaacca accgtaatca acggtcagat ccaggtgcgg
     2341 agcaacagct gccattttgt cctggatttc cgggccaacc cacagggtgc tgttcatcgc
     2401 gccagtctga ccaggctgaa ccagtaccgg ctgagattta tagccgatag cggcgatgcc
     2461 gttacccaga ttagcggtat agaagttgtt ggtaccgtcg ttatgcggga tccacgccgt
     2521 cgcgaaatac tgttgcagca tcgccaccca accacctttc gaagagatgt tcaggttttc
     2581 gttatcggca atggtatcga acttgtattt ctcatacttc tcgtcaggcg tggagtacgc
     2641 cgcgccacgg aaggtgtgca gtgcgaagtt gctgcttccg gtatcgagat gcggtggcag
     2701 agtgatggat tgcttcaact gaccaaaggt ggagatttcc agcggtttct cgccagcgtt
     2761 ctgcacgttg tagttgacgt tgacagcgta atcaccacgt ttcaggacaa acgttttggt
     2821 aaacgtgttg cctgccgcgt cggtatacgt catcggcacc tgcagttcgt tttgaccttc
     2881 agccagcaca taagcgtctt tttcaacgtt atacagcgga cgcgggccgt tagccgggtt
     2941 atccgggcca tcacgaccgg tcagaccgct ctgtgcctga taaataaact gcggtgaagt
     3001 ttccagcagc tggaacggct gggtagagtt cagctctttc gggtaagcag gcagcagagc
     3061 ttgctcaaca tcaccaccac gggtgttgat ggtcagatca agcacgtcgg tcttaaccga
     3121 gatcagtttc ccctggccac tggccggtac gccctggtcg gcggcgctac ccgctgcggt
     3181 ggtcgttgtc tgcgtggtct gttgggcctg aggttgcggg tttttatcct gctcccaggc
     3241 ttgccagatc atgaaagaca cgaacagcaa agcgatgact aaaagattgc gttgcgaatc
     3301 catcgttagt gttctctggt atcaaatggt ccgggcggga cgggatcgtc accaccaggg
     3361 tgtaaagggt ggcattttaa tacgcgtttc accgtcaacc aactgccttt tatcactcca
     3421 aacctgcgca atgcctcaat tccgtagctt gaacaggttg gagtgaaacg acaatgcggc
     3481 ccgagtagcg gactaatcag gcgttgatag acccgaatga gggctatcag gacccgcgag
     3541 ccaggcgaca gtggcggcgc cataattttt ccaacgcttc cgagagagca cggttatcga
     3601 ggtcggcaac cccttttttc gccaccacca cgaaatccat agccgggagt tcatgttggc
     3661 gcagacggaa gctttcacgc gtcagacgtt taatccgatt gcgttcatgg gcgcgtcgaa
     3721 cgtttttctt ggcgactgta agaccgatac ggggatgccc cagcgaattc aggcggccga
     3781 gaatggtaat ttgcggcgtg ccagcccgtt gtggctgctg gaagacgaat gtgaattgac
     3841 tgggagttaa caagcgtaac tccctgggaa atgcgagctt aaccactcag gggttagctt
     3901 tattacttag aaacggtcag acgagcgcgg cctttagcac gacgacgtgc cagaacctga
     3961 cgaccatttt tagtagccat acgagcacgg aagccgtgag aacggttgcg cttcagtaca
     4021 gacggttgaa aagtgcgttt catggcgatt tctacctaaa cttgaataaa ttcaatggct
     4081 ttattggata tccgccgaaa aatgaaacga tggacaccga agccatgggt gattaaagag
     4141 gccggattgt aataattgta cactccggag tcaattctct ttccttattt accgcgcttt
     4201 tccgcacctt ttcgcaggga aaatgtacga cctcacacca gtgaaaacca gcatggcgcg
     4261 ccgggtggag gattatacgg gctgatgggt aaagcgcaag gatcgtcctg gatctttatt
     4321 agatcgatta agccaatttt tgtctatggt cattaaattt tccaatatgc ggcgtaaatc
     4381 gtgcccgcct cgcggcagga tcgtttacac ttagcgaggt ctggaaagtc ctgtggataa
     4441 atcgggaaaa tctgtgagaa acagaagatc tcttgcgcag tttaggctat gatccgcggt
     4501 cccgatcgtt ttgcaggatc ttgatcgggc atataaccgc agacagcggt tcgtgcgtca
     4561 ccctcaagca gggtcttttc gacgtacgtc aacaatcatg aatgtttcag ccttagtcat
     4621 tatcgacttt tgttcgagtg gagtccgccg tgtcactttc gctttggcag cagtgtcttg
     4681 cccgattgca ggatgagtta ccagccacag aattcagtat gtggatacgc ccattgcagg
     4741 cggaactgag cgataacacg ctggccctgt acgcgccaaa ccgttttgtc ctcgattggg
     4801 tacgggacaa gtaccttaat aatatcaatg gactgctaac cagtttctgc ggagcggatg
     4861 ccccacagct gcgttttgaa gtcggcacca aaccggtgac gcaaacgcca caagcggcag
     4921 tgacgagcaa cgtcgcggcc cctgcacagg tggcgcaaac gcagccgcaa cgtgctgcgc
     4981 cttctacgcg ctcaggttgg gataacatcc cggccccggc agaaccgacc tatcgttcta
     5041 acgtaaacgt caaacacacg tttgataact tcgttgaagg taaatctaac caactggcgc
     5101 gcgcggcggc tcgccaggtg gcggataacc ctggcggtgc ctataacccg ttgttccttt
     5161 atggcggcac gggtctgggt aaaactcacc tgctgcatgc ggtgggtaac ggcattatgg
     5221 cgcgcaagcc gaatgccaaa gtggtttata tgcactccga gcgctttgtt caggacatgg
     5281 ttaaagccct gcaaaacaac gcgatcgaag agtttaaacg ctactaccgt tccgtagatg
     5341 cactgctgat cgacgatatt cagttttttg ctaataaaga acgatctcag gaagagtttt
     5401 tccatacctt caacgccctg ctggaaggta atcaacagat cattctcacc tcggatcgct
     5461 atccgaaaga gatcaacggc gttgaggatc gtttgaaatc ccgcttcggt tggggactga
     5521 ctgtggcaat cgaaccgcca gagctggaaa cccgtgtggc gatcctgatg aaaaaggccg
     5581 acgaaaacga cattcgtttg ccgggcgaag tggcgttctt tatcgccaag cgtctacgat
     5641 ctaacgtacg tgagctggaa ggggcgctga accgcgtcat tgccaatgcc aactttaccg
     5701 gacgggcgat caccatcgac ttcgtgcgtg aggcgctgcg cgacttgctg gcattgcagg
     5761 aaaaactggt caccatcgac aatattcaga agacggtggc ggagtactac aagatcaaag
     5821 tcgcggatct cctttccaag cgtcgatccc gctcggtggc gcgtccgcgc cagatggcga
     5881 tggcgctggc gaaagagctg actaaccaca gtctgccgga gattggcgat gcgtttggtg
     5941 gtcgtgacca cacgacggtg cttcatgcct gccgtaagat cgagcagttg cgtgaagaga
     6001 gccacgatat caaagaagat ttttcaaatt taatcagaac attgtcatcg taaacctatg
     6061 aaatttaccg tagaacgtga gcatttatta aaaccgctac aacaggtgag cggtccgtta
     6121 ggtggtcgtc ctacgctacc gattctcggt aatctgctgt tacaggttgc tgacggtacg
     6181 ttgtcgctga ccggtactga tctcgagatg gaaatggtgg cacgtgttgc gctggttcag
     6241 ccacacgagc caggagcgac gaccgttccg gcgcgcaaat tctttgatat ctgccgtggt
     6301 ctgcctgaag gcgcggaaat tgccgtgcag ctggaaggtg aacggatgct ggtacgctcc
     6361 gggcgtagcc gtttttcgct gtctaccctg ccagcggcgg atttcccgaa cctcgatgac
     6421 tggcagagtg aagtcgaatt taccctgccg caggcaacga tgaagcgtct gattgaagcg
     6481 acccagtttt cgatggcgca tcaggacgtt cgctattact taaatggtat gctgtttgaa
     6541 accgaaggtg aagaactgcg caccgtggca accgacggcc accgtctggc ggtctgttca
     6601 atgccaattg gtcaatcttt gccaagccat tcggtgatcg taccgcgtaa aggcgtgatt
     6661 gaactgatgc gtatgctcga cggcggcgac aatccgctgc gcgtgcagat tggcagcaac
     6721 aatattcgcg cccacgttgg cgactttatc ttcacctcca aactggtgga tggtcgcttc
     6781 ccggattacc gccgcgttct gccgaagaat ccggacaaac atctggaagc tggctgcgat
     6841 ctgctcaagc aggcgtttgc ccgtgcggca attctctcta acgagaaatt ccgcggcgtg
     6901 cgcctgtatg tcagcgaaaa ccagctgaaa atcaccgcca acaacccgga acaggaagaa
     6961 gcggaagaga tcctcgacgt tacctatagc ggtgcggaga tggaaatcgg cttcaacgtc
     7021 agctatgtgc tggatgttct gaacgcgctg aaatgcgaaa acgtccgcat gatgctgacc
     7081 gattcggttt ccagcgtgca gattgaagat gccgcatcac agtcggctgc ctatgttgtc
     7141 atgccaatga gactgtaatg tccctcaccc gcttgttgat ccgcgatttc cgcaacattg
     7201 aaaccgcgga tctcgcttta tctcccggct ttaactttct ggtaggtgcc aacggcagtg
     7261 gcaaaaccag cgtgctggaa gccatctata cgctcggcca tggtcgggcg tttcgcagtt
     7321 tgcagattgg tcgcgtcatt cgccatgagc aggaggcatt tgttctccat gggcgattac
     7381 agggcgaaga gcgcgagacg gcgattggct taaccaagga caaacagggc gacagcaaag
     7441 tccgcatcga cggtactgac gggcataaag tcgcggaact ggcgcacctg atgccaatgc
     7501 agctgataac gccagaaggg tttactttac tcaacggcgg ccccaaatac agaagagcat
     7561 tcctcgactg gggatgcttt cacaacgaac ccggattttt caccgcctgg agcaatctca
     7621 agcgattgct caagcagcgc aatgcggcgc tgcgccaggt gacacgttac gaacagctac
     7681 gcccgtggga taaagaactg atcccgctgg cggagcaaat cagcacctgg cgcgcggagt
     7741 atagcgccgg tatcgcggcc gatatggccg atacctgtaa gcaatttctc cctgagtttt
     7801 ctctgacttt ctctttccag cgcggctggg agaaagagac agaatatgct gaggtgctgg
     7861 aacgtaattt tgaacgcgat cgccagctaa cctacaccgc gcatggcccg cataaagcgg
     7921 acttacgcat tcgcgccgac ggtgcgccgg tggaagatac cttatcgcgt gggcagctta
     7981 agctgttgat gtgcgcctta cgtctggcgc aaggagagtt cctcacccgt gaaagcgggc
     8041 ggcggtgtct ctacctgata gatgattttg cctctgagct tgatgatgag cgtcgtgggt
     8101 tgcttgccag ccgcttaaaa gcgacgcaat cacaggtctt tgtcagcgcg atcagtgctg
     8161 aacacgttat agacatgtcg gacgaaaatt cgaagatgtt taccgtggaa aagggtaaaa
     8221 taacggatta acccaagtat aaatgagcga gaaacgttga tgtcgaattc ttatgactcc
     8281 tccagtatca aagtcctgaa agggctggat gcggtgcgta agcgcccggg tatgtatatc
     8341 ggcgacacgg atgacggcac cggtctgcac cacatggtat tcgaggtggt agataacgct
     8401 atcgacgaag cgctcgcggg tcactgtaaa gaaattatcg tcaccattca cgccgacaac
     8461 tctgtctctg tacaggatga cgggcgcggc attccgaccg gtattcaccc ggaagagggc
     8521 gtatcggcgg cggaagtgat catgaccgtt ctgcacgcag gcggtaaatt cgacgataac
     8581 tcctataaag tgtccggcgg tctgcacggc gttggtgttt cggtagtaaa cgccctgtcg
     8641 caaaaactgg agctggttat ccagcgcgag ggtaaaattc accgtcagat ctacgaacac
     8701 ggtgtaccgc aggccccgct ggcggttacc ggcgagactg aaaaaaccgg caccatggtg
     8761 cgtttctggc ctagcctcga aactttcacc aatgtgaccg agttcgaata tgaaattctg
     8821 gcgaaacgtc tgcgtgagtt gtcgttcctc aactccggcg tttccattcg tctgcgcgac
     8881 aagcgcgacg gcaaagaaga ccacttccac tatgaaggcg gcatcaaggc gttcgttgaa
     8941 tatctgaaca agaacaaaac gccgatccac ccgaatatct tctacttctc cactgaaaaa
     9001 gacggtattg gcgtcgaagt ggcgttgcag tggaacgatg gcttccagga aaacatctac
     9061 tgctttacca acaacattcc gcagcgtgac ggcggtactc acctggcagg cttccgtgcg
     9121 gcgatgaccc gtaccctgaa cgcctacatg gacaaagaag gctacagcaa aaaagccaaa
     9181 gttagcgcca ccggtgacga tgcgcgtgaa ggcctgattg cggtcgtttc cgtgaaagtg
     9241 ccggacccga aattctcctc ccagaccaaa gacaaactgg tttcttctga ggtgaaatca
     9301 gcggttgaac agcagatgaa cgaactgctg gcagaatacc tgctggaaaa cccaaccgac
     9361 gcgaaaatcg tggttggcaa aattatcgat gctgcccgtg cccgtgaagc ggcgcgtcgc
     9421 gcgcgtgaaa tgacccgccg taaaggtgcg ctcgacttag cgggcctgcc gggcaaactg
     9481 gcagactgcc aggaacgcga tccggcgctt tccgaactgt acctggtgga aggggactcc
     9541 gcgggcggct ctgcgaagca ggggcgtaac cgcaagaacc aggcgattct gccgctgaag
     9601 ggtaaaatcc tcaacgtcga gaaagcgcgc ttcgataaga tgctctcttc tcaggaagtg
     9661 gcgacgctta tcaccgcgct tggctgtggt atcggtcgtg acgagtacaa cccggacaaa
     9721 ctgcgttatc acagcatcat catcatgacc gatgcggacg tcgacggctc gcacattcgt
     9781 acgctgctgt tgaccttctt ctatcgtcag atgccggaaa tcgttgaacg cggtcacgtc
     9841 tacatcgctc agccgccgct gtacaaagtg aagaaaggca agcaggaaca gtacattaaa
     9901 gacgacgaag cgatggatca gtaccagatc tctatcgcgc tggatggcgc aacgctgcac
     9961 accaacgcca gcgcaccggc attggctggc gaagcgttag agaaattggt gtctgagtac
    10021 aacgcgacgc agaaaatgat caaccgcatg gagcgtcgtt atccgaaagc aatgctgaaa
    10081 gagcttatct atcagccgac gctgacggaa gccgacctct ctgatgagca gaccgttacc
    10141 cgctgggtga acgcgctggt cagcgaactg aacgacaaag aacagcacgg cagccagtgg
    10201 aagtttgatg tccacaccaa tgccgaacaa aacctgttcg agccgattgt tcgcgtgcgt
    10261 acccacggtg tggatactga ctatccgctg gatcacgagt ttatcaccgg tggcgaatat
    10321 cgtcgtatct gcacgctggg tgagaaactg cgtggcttgc tggaagaaga tgcgtttatc
    10381 gaacgtggcg agcgtcgtca gccggtagcc agcttcgagc aggcgctgga ctggctggtg
    10441 aaagagtccc gtcgcggcct ctccatccag cgttataaag gtctgggcga gatgaacccg
    10501 gaacagctgt gggaaaccac catggacccg gaaagccgtc gtatgctgcg cgttaccgtt
    10561 aaggatgcga ttgccgctga ccagttgttc accacgctga tgggcgacgc cgttgaaccg
    10621 cgccgtgcgt ttattgaaga gaacgccctg aaagcggcga atatcgatat ttaatggcgc
    10681 taaccatgcg agcgtgcctg atgcgctacg cttatcaggc ctacgaaaat cttgcaatgt
    10741 attgaatttt catgattttg taggccggat aaggcgttca cgccgcatcc ggcaagaagc
    10801 tataagaaaa gggcggagat catctccgcc ctttttattt ctgcaatccg caattatcat
    10861 cccgcaaatc tatacttttt gggtcaacac gtaacgcttt acaggtgatt atcatggggc
    10921 tttttgatga agttgtcggt gcctttctga aaggcgatgc ggggaaatat caggctattt
    10981 taagttgggt tgaggagcag ggcggcattc aggtgctgct ggaaaaactg caaagtggcg
    11041 gcttaggggc cattctctca acctggctga gtaatcaaca gggcaatcaa tcggttagtg
    11101 gcgagcaact ggaatcggcg ctcggcacaa atgcggtgtc cgatcttgga caaaaacttg
    11161 gcgtggatac cagtacagct tccagtttac tggcagaaca attgccgaag attattgatg
    11221 cgctctcacc gcaaggtgaa gtgtctccac aagccaataa cgatctgctt tccgcaggca
    11281 tggaactgct gaaagggaaa ctcttccgct aagcaaatgg ggagcatgca acccgcgttg
    11341 catccccctt ttccctcggg tgttgtactg atttttgagc ggaatcgcgt tagcatgggt
    11401 caggaactaa tctacctggg gaactcatgg ctatcaaact cattgctatc gatatggatg
    11461 gcacccttct gctacccgat cacaccattt cacccgccgt taaaaatgcg attgccgcag
    11521 ctcgcgcccg tggcgtgaat gtcgtgctaa cgacgggtcg cccttatgca ggtgttcaca
    11581 attacctgaa agagctgcat atggaactgc cgggcgacta ctgcattact tataacggcg
    11641 cgctggtaca gaaggccgct gatggtagca ccgtggcgca aaccgctctc agctatgacg
    11701 actaccgttt cctggaaaaa ctctctcgcg aagtcggttc tcatttccac gccctggacc
    11761 gcaccacgct gtacaccgcc aaccgtgata tcagctacta cacggtgcat gaatccttcg
    11821 ttgccacaat tccgctggtg ttctgcgaag cggagaaaat ggaccccaat acccagttcc
    11881 tgaaagtgat gatgattgat gaacccgcca tcctcgacca ggctatcgcg cgtattccgc
    11941 aggaagtgaa agagaaatat accgtgctga aaagtgcgcc gtacttcctc gaaatcctcg
    12001 ataaacgcgt taacaaaggt acgggggtga aatcactggc cgacgtgtta ggtattaaac
    12061 cggaagaaat catggcgatt ggcgatcagg aaaacgacat cgcaatgatt gaatatgcag
    12121 gcgtcggtgt ggcgatggat aacgctattc cttcggtgaa agaagtggcg aactttgtca
    12181 ccaaatccaa ccttgaagat ggcgtggcgt ttgctattga gaagtatgtg ctgaattaat
    12241 cagtggacgg gcaaacagca taaacgcgtt tgcccgctta ctgattattg gtttactgca
    12301 tatacaacca gaaattgcag cggtgcagta atgatgtcca gcgtaacagt cactggcagt
    12361 aatgctgcgg aaacaccatc cggaatgcct ctgggtgaat agtattcata aaatcgcact
    12421 ttaaacggat gatagaacgc catcaccttt gagtaatcca tgtttttatt tttctcatgg
    12481 attgtgcctt ttaggttttc cagtgacctg gtacagtgtt gaacaccaca ggcaaaacca
    12541 taatgcgttg ccagtgcctt ttcttcttcg ttattccagt ggtagtaaag cgatattgtg
    12601 ccggtaaatt tctttttccc cgcatttaaa acaaatcttg cgtcatcggc aacctgaata
    12661 ttgtgccggt taagtgctgg atcattgagc atcttaacga tgtcatcggc accatcagtg
    12721 ataaggtaat ccagcgattc ccctacaaaa acatagcctt ttgttccatt actatctttt
    12781 gcctgcgata gcccagtgat ggtgtctgac tgcgattgat actttcctga actacccttt
    12841 gcggtatggg aaaccagagc atacgagata cagcctgaaa gcatcagcgc cagtgggaat
    12901 aaaccggcag ccttaaaaaa tcccttaaaa ttcaacttca tgattaatcc tttaaacgag
    12961 tagctaagcc gttgaaagca tactcttctt tccctcctgt ttagcgcctg atatcccttt
    13021 tcagatttct gcccgacgca tgtcattttt ttatgcattg ttctttttgt gatctaaatt
    13081 gtagtacaac aatataagtt tgtactacat tacgcacacg gcaaacgcga acgtcatcac
    13141 gctggtacta caaagttgcc gcgttatgca tcgatcgggg taaagtagag aagaacatac
    13201 agagcacaag gactctccat gactctcaat aaaaccgatc gcattgtcat tacgctgggt
    13261 aaacagatcg ttcacggcaa atacgtacct ggctcgccac ttccggctga ggcggagctc
    13321 tgtgaagagt ttgcaacctc gcgcaacatc atccgtgagg tgttccgttc gttgatggcg
    13381 aaacggctga ttgaaatgaa acgttatcgc ggcgcgtttg tggcaccgcg taaccagtgg
    13441 aattacctcg acactgacgt actgcaatgg gtgctggaaa acgactacga cccacggctt
    13501 atcagtgcca tgagcgaagt gcgaaatctg gtggaaccgg cgattgcccg ttgggcagca
    13561 gagcgcgcga cttccagcga tctggcgcag attgaatcgg cgctgaacga gatgattgcc
    13621 aacaatcagg accgcgaagc gtttaacgaa gcggatattc gctaccacga ggcggtgctg
    13681 cagtcggtac ataacccggt gttacagcaa cttagcattg cgatcagttc gttgcagcgg
    13741 gcggtttttg aacgaacctg gatgggcgat gaggccaaca tgccgcaaac gctccaggaa
    13801 cataaggcgc tgttcgatgc aatacggcat caggacggcg atgcggcaga gcaggcggcg
    13861 cttaccatga tcgccagctc gacacgaagg ttaaaggaaa tcacatgaca gctcgctaca
    13921 tcgcaattga ctggggatcg accaatcttc gcgcctggct ttatcagggc gaccactgcc
    13981 tggagagcag gcaatcagaa gcaggcgtca cgcgcctgaa cggaaaatct ccggctgcgg
    14041 tgttagcaga agtcacgacc gactggcgtg aagagaatac gccagtggta atggcaggaa
    14101 tggtcggcag caatgtcggc tggaaagttg ctccgtattt atctgttcct gcccgttttt
    14161 cgtctattgg cgaacaatta acgtctgttg gcgacaatat ctggattatt cccggattat
    14221 gcgtctctca tgacgataac cacaatgtga tgcgcggcga agaaacacaa ttgatcggcg
    14281 cgcgaactct ggctccttcc tctctttatg ttatgcccgg tacgcattgc aaatgggtgc
    14341 aggccgatag ccagcaaatc aacgattttc gcaccgtgat gaccggtgaa ttacatcatt
    14401 tactgttaaa tcactcattg attggcgcag gtttgccgcc gcaggaaaac tctgccgatg
    14461 ccttcgcggc tggccttgag cgcggcctta atgcgcccgc catattgccg cagctttttg
    14521 aagttcgcgc ctcgcatgtg ctgggaacac ttccccgcga acaggtcagc gaatttctct
    14581 ctggtttgtt gattggcgca gaggttgcca gtatgcgcga ctatgtgacc catcaacacg
    14641 ccatcaccct tgtcgccgga acatcgctga ccgcgcgcta ccagcaagcc tttcaggcga
    14701 tgggttgcga cgtgacggcg gtggcgggcg acacggcatt tcaggctggt ataaggagca
    14761 tcgctcatgc agtggcaaac taaacttccg ctgatcgcca ttttgcgcgg cattaagccc
    14821 gacgaggcgc tggcgcatgt tggcgcggtg attgacgccg ggttcgacgc ggttgaaatc
    14881 ccgctgaatt ccccacaatg ggagcaaagc attcccgcca tcgttgatgc gtatggcgac
    14941 aaggcgttga ttggcgcagg tacggtactg aaacctgaac aggtcgatgc gctcgccagg
    15001 atgggttgtc agctcatcgt tacgcccaat atccatagtg aagtgatccg ccgtgcggtg
    15061 ggctacggca tgaccgtctg ccccggctgc gcgacggcga ccgaagcctt taccgcgctc
    15121 gaagcgggcg cgcaggcgct gaaaatattt ccgtcatcgg cttttggtcc gcaatacatc
    15181 aaagcgttaa aagcggtatt gccatcggac atcgcagtct ttgccgttgg cggcgtgacg
    15241 ccagaaaacc tggcgcagtg gatagacgca ggttgtgctg gggcgggctt aggcagcgat
    15301 ctctatcgcg ccgggcagtc cgtagaacgc accgcgcagc aggcagcagc atttgttaag
    15361 gcgtatcgag aggcagtgca atgaaaatca ccaaaattac cacgtatcgt ttacctcccc
    15421 gctggatgtt cctgaaaatt gaaaccgatg aaggcgtggt cggttggggc gagcccgtga
    15481 ttgaaggccg cgcccgtacg gtggaagcgg cagttcacga gctgggtgac tatttgattg
    15541 gtcaggatcc ttcgcgcatc aatgacttat ggcaagtgat gtatcgcgcc ggattttatc
    15601 gtggcggtcc aatcctgatg agcgccattg ccgggatcga ccaggcgtta tgggatatca
    15661 aaggcaaagt gctgaatgcg ccggtctggc aactgatggg cggcctggtt cgcgacaaaa
    15721 ttaaagccta cagttgggtc ggcggcgatc gtccggcgga tgttatcgac ggcattaaaa
    15781 ccctgcgcga aatcggcttc gataccttca aactgaacgg ttgtgaagaa ctggggctaa
    15841 ttgataactc ccgcgcggta gatgcggcag tcaacaccgt ggcacaaatt cgtgaagctt
    15901 ttggcaatca gattgagttt ggtcttgatt tccacggtcg cgtcagcgcg ccaatggcga
    15961 aagtgctgat taaagaactg gagccgtatc gcccgctgtt tattgaagag ccggtgctgg
    16021 cggaacaagc cgaatactac ccgaaattgg cggcacaaac gcatattcca ctggcggcag
    16081 gtgagcgcat gttctcacgt ttcgatttta aacgcgtgct ggaggcaggc ggtatttcga
    16141 ttctgcaacc ggatctctcc catgcaggcg gtattaccga atgctacaaa attgctggaa
    16201 tggcagaagc ctatgatgtg acccttgcgc cgcactgtcc gctcggaccg attgcactgg
    16261 cggcttgcct gcatatcgac tttgtttcct ataacgcggt acttcaggaa caaagtatgg
    16321 gcattcatta caacaaaggc gcggagttac tcgactttgt gaaaaacaaa gaagacttca
    16381 gtatggttgg cggcttcttt aaaccgttaa cgaaaccggg cttaggtgtg gaaatcgacg
    16441 aagctaaagt tattgagttc agtaaaaatg ccccggactg gcgtaatccg ctctggcgtc
    16501 atgaagataa cagcgtagca gagtggtaat tcctgccacg taagcccctc atcgggcact
    16561 aaaacagcaa tacaaaaata taaccctctg taaattacag ggcatggtga gcggcttcgc
    16621 tatgcccaaa atctggagaa agctcgctat ggatattccc gttaatgcag caaagccggg
    16681 gcgtcggcgt tatctgacgc tggtgatgat ctttattacg gtggtcattt gttatgttga
    16741 ccgcgctaac ctggccgtgg cttccgccca tattcaggaa gagttcggca ttacgaaagc
    16801 ggaaatgggc tatgtatttt cggccttcgc ctggctttat acgctatgcc agatccccgg
    16861 cggttggttt ttagatcgcg taggttctcg cgtgacttat tttattgcga tatttggttg
    16921 gtcagtggcg actttattcc agggctttgc cacgggatta atgtcattaa ttggtctgcg
    16981 tgcgataacc ggtattttcg aagcgcccgc tttcccgacc aataaccgga tggtgaccag
    17041 ctggttcccg gaacatgaac gcgcttccgc cgttggtttt tatacgtctg gtcagtttgt
    17101 cggtctggcg tttttgactc cgctgctgat ctggattcag gagatgttga gctggcactg
    17161 ggtgttcatt gtcactggtg gtatcggcat tatctggtcg ctgatttggt ttaaggttta
    17221 tcagccgccg cgcctgacca aaggtatcag caaagctgaa ctggattaca ttcgtgatgg
    17281 cggcggtctg gtggatggtg atgcgccggt gaagaaagag gcgcgtcagc cgttaacagc
    17341 caaagactgg aaactggtgt tccatcgtaa actgatcggc gtttatcttg ggcaatttgc
    17401 ggtggcttct acactgtggt ttttcttaac ctggttcccg aactatttaa cccaggaaaa
    17461 aggaatcacg gcgctgaaag caggctttat gaccacggtg ccattcctcg cggcgtttgt
    17521 cggcgtcctg ctctctggct gggtcgcgga tctgctggta cgtaagggct tttcactggg
    17581 ctttgcgcgt aaaacgccga ttatctgcgg cttgctgatc tccacctgca ttatgggcgc
    17641 taactacacc aacgatccga tgatgattat gtgcctgatg gcgctggcat tcttcggcaa
    17701 cggttttgct tcgattacct ggtcgctggt ctcttctctg gcaccgatgc gcctgattgg
    17761 tttaaccggc ggcgtgttta acttcgccgg tggtctgggc ggcatcaccg ttccgctggt
    17821 ggtggggtac ctggcgcagg gttacggttt cgcacctgca ctggtttata tctccgccgt
    17881 cgcgttgatt ggcgcgctct cttacatcct gctggtgggc gatgtgaagc gcgttggcta
    17941 atctttcaac tgtggaatat gcgccatacc actgcgcata atccgctttc ttaaagccgg
    18001 tgcggtcagg catcggcttt ttactatctt gccgaagagt tttaaacgca gtttgcgggt
    18061 ggcgcgccag taagcggtat tgagcttctc tggctgcttc agtaacaccg aacgcagaat
    18121 gtctgcgcta tccagcgcat agctaatccc ctccagcgaa ctggcgctga taaatcctgc
    18181 cgcttcgcca atcagaaagg cgttgtcctt accgcagaca aaatcctgcc agcgtgaggg
    18241 aaacagcacc gtgcattttt cgcttttcac cgccttacca aactggaact gaaaggcgct
    18301 cattttctct ttcagcgtcg tgaaacgcgt ctgaccgtct ttcattggat aagcaccgcc
    18361 aaagataaaa taaccgtctt tgctgatact ccatgaataa cagttagtta tcgaattatc
    18421 aaagatgcag gagtagaacg gcaccggatg tttctctgcg aaccactgct ggatagcgac
    18481 atatttacgg atttgatgat ccgggtagag atgtcgccgc accatcgagt ttgcgccatc
    18541 ggcaccgacc agatagcggg cagtaatatg ttgctcccag ccgtctgcac gaaaaatgac
    18601 atgccattta tcatcctcac gccagatttt tcgacacagg ctgtcgtgat aaatctcaac
    18661 gctggcgggg atcagcgatt tcatccacaa gtcgaaagca tggcgattaa tattgatata
    18721 gcttcgctgg tagttacgcg tgagcgatgc ggcgacgtcg acagttttga cgctgaaaat
    18781 ctgcgggttg gcgatcacat cgacaggaag cgtcagtcca tcgcgaataa aagatcgctg
    18841 cgcgtccggt gccagcagac cgccgcaagg tttgctgaaa ccttcagtac cacactggtg
    18901 ctttttatcc agcgcgatca cctgcatttt gcctgctaat tttcgcgcca acgccgatcc
    18961 tgccgggccg aggccaataa tcgccacgtc gaaatgttcc atcttttcac tcacattcat
    19021 cacgatggca aaagactatt gtctgaaagt gaagtccttg tgaagtatga tggcgggacc
    19081 cgttttgtat caggaccgcg ccatgaaaca gataaccttt gctccccgta atcacctgct
    19141 caccaatacc aatacctgga cgcccgacag ccagtggctg gtatttgacg tgcgtccttc
    19201 tggggcgtcg tttaccggcg agaccattga gcgtgtgaat atccataccg gcgaggtcga
    19261 ggttatctat cgcgcgtcac agggcgcaca cgtcggcgtg gtgaccgttc atccgaagtc
    19321 agagaaatat gttttcatcc atggcccgga aaatcctgat gaaacatggc attacgattt
    19381 ccatcatcga cgcggagtca ttgctgaagg cggcaaggtg agcaatctcg atgcaatgga
    19441 tattaccgcg ccgtatatgc caggagcgct gcgcggcggc agccatgtgc atgtctttag
    19501 cccgaacggt gaaagggtga gctttaccta taacgaccat gtaatgcatg aactcgatcc
    19561 ggcgctggat ttgcgaaacg tcggtgttgc tgcgccgttt ggcccggtca acgtacaaaa
    19621 gcagcatccg cgtgaataca gcggtagcca ctggtgcgtg ctggtgagta aaaccacgcc
    19681 cacgccgcag cctggcagcg atgaaatcaa tcgtgcttat gaagaaggat gggtaggaaa
    19741 tcacgcgctg gcgtttattg gcgacacact ttcgccaaag ggcgagaaag tgccggagct
    19801 gtttatcgtt gagttaccgc aagatgaagc tggctggaaa gcggcaggtg atgcgccgtt
    19861 aagtggaacg gaaaccaccc tgcccgcgcc accgcgcggc gtcgtgcagc gacgtttaac
    19921 ctttacccac catcgggctt atccggggtt agtcaacgtc ccgcgccact gggtgcgctg
    19981 taatccgcag ggtacgcaaa tcgcgttttt aatgcgtgat gataacggca ttgtgcaact
    20041 gtggcttatc tcgccacagg gcggcgagcc gcgccagtta acccataaca aaacggatat
    20101 tcagtctgca tttaactggc atccgtcagg agaatggttg ggctttgtgc tggataatcg
    20161 aattgcttgt gcccatgcgc aaagcggcga ggttgagtat ttaaccgaaa accacgccaa
    20221 tccaccttct gcggacgccg aggtcttctc gccggatggt caatggctgg cgtggatgga
    20281 aggtggccag ctgtggatca ccgaaactga tcgctaaatc agttatcagg cattctggcc
    20341 ggtggaataa ccgcattggt ggcttgcgcg ttggcttcgc ttttttcaac gcgtgagcgc
    20401 accgagctgt ctttgcggaa cacatcccac ggcagtaaaa gcgtgtccat cacagcggta
    20461 aacggcatat cgagaatcgc cagcgactta gtgccccagt tggtttcatc gtcgccaatc
    20521 atggttgcgc tggcgcgggt gccgggatac gttccttctt tccctccggt atgggacatc
    20581 acgctggagc atcctccgag taatgtcatt ccgctgcata tcatgaacgc taacaacaca
    20641 tttcttatca taataaattc atctgttgat cgtgggtgtt ggcctgatga gttatagcga
    20701 tcccttgctg aaaataacat catcattacg tcgcactgtg gcggctatcg cactttaacg
    20761 tttcgtgctg ccccctcagt ctatgcaata gaccataaac tgcaaaaaaa agtccgctga
    20821 taaggcttga aaagttcatt tccagaccca tttttacatc gtagccgatg aggacgcgcc
    20881 tgatgggtgt tctggctacc tgacctgtcc attgtggaag gtcttacatt ctcgctgatt
    20941 tcaggagcta ttgattatgc gtaactttga tttatccccg ctttaccgtt ctgctattgg
    21001 atttgaccgt ttgtttaacc acttagaaaa caaccagagc cagagtaatg gcggctaccc
    21061 tccgtataac gttgaactgg tagacgaaaa ccattaccgc attgctatcg ctgtggctgg
    21121 ttttgctgag agcgaactgg aaattaccgc ccaggataat ctgctagtgg tgaaaggtgc
    21181 tcacgccgac gaacaaaaag agcgcaccta tctgtaccag ggcatcgctg aacgcaactt
    21241 tgaacgcaaa ttccagttag ctgagaacat tcatgttcgt ggcgctaacc tggtaaatgg
    21301 tttgctgtat atcgatctcg aacgcgtgat tccggaagcg aaaaaaccgc gccgtatcga
    21361 aatcaactaa ttccctaagg ccgcctggcg cggcctgaca tctccatgct cgccgttagg
    21421 gagcatatgc gaatcttcgg atttgcaggt acttactcgc ttcttagaag gagaaatgac
    21481 tatgcgtaac ttcgatttat ccccactgat gcgtcaatgg atcggttttg acaaactggc
    21541 caacgcactg caaaacgccg gtgaaagcca gagcttcccg ccgtacaaca ttgagaaaag
    21601 cgacgataac cactaccgca ttacccttgc gctggcaggt ttccgtcagg aagatttaga
    21661 gattcaactg gaaggtacgc gcctgagcgt aaaaggcacg ccggagcagc caaaagaaga
    21721 gaaaaaatgg ctgcatcaag ggcttatgaa tcagccattt agcctgagct ttacgctggc
    21781 tgaaaatatg gaagtctctg gcgcaacctt cgtaaacggt ttactgcata ttgatttaat
    21841 tcgtaatgag cctgaaccca tcgcagcgca gcgtatcgct atcagcgaac gtcccgcgtt
    21901 aaatagctaa ctagctattc tctttgcccc gccattctgg tggggctttt tttgtcttac
    21961 ttactcaaca atgcgttaaa tttcgactgt ttaagatatt tcggcacgtt ctttcccgtg
    22021 ataatgtgcg ctataaggca aatctgcttc accgattcag ccataatccg tagcaattaa
    22081 aaatgttatc tcaagagaaa tggacgatga gtgatatagc attaacggtc agtattctgg
    22141 ctttggtggc agtcgtcggt ttgtttatcg gcaatgtcaa atttcgcggc ataggattag
    22201 gtattggcgg cgtgctgttt ggtgggatca tcgtcggcca ttttgtttct caggcgggga
    22261 tgacattaag tagcgatatg ctgcatgtta ttcaggaatt tggcctgatc ctgttcgttt
    22321 atactatcgg gattcaggta gggccgggct tctttgcctc attgcgcgtc tccggattac
    22381 gcctcaacct gtttgctgtt ctgatcgtca tcatcggtgg tctggttacc gccatcctgc
    22441 ataaactgtt tgatatgcca ctgccggtag tgctggggat tttctccggt gcggttacca
    22501 atacgccagc gctgggggca gggcagcaga ttttgcgcga cttgggtaca ccaatggaaa
    22561 tggtcgatca gatggggatg agttacgcga tggcgtatcc attcggcatt tgcgggattt
    22621 tgttcaccat gtggatgttg cgggttattt tccgcgtcaa tgtcgagaca gaagctcagc
    22681 agcacgagtc ttcacgcacc aatggcggcg cgctgatcaa gactatcaat attcgcgttg
    22741 agaaccctaa cctgcatgat ttagccatta aagatgtacc gattctcaac ggcgacaaaa
    22801 ttatctgctc gcgtctgaaa cgcgaagaaa ccctaaaagt tccttcgcca gataccatta
    22861 tccaactggg cgatttgctg catctggtgg ggcagccagc ggatttacat aatgcgcaac
    22921 tggtgattgg tcaggaggtc gatacttcgc tgtccacgaa aggcactgat ttgcgcgtcg
    22981 agcgtgtggt ggtcaccaat gaaaacgtgc tcggaaaacg tattcgcgac ctgcacttta
    23041 aagaacgcta tgacgttgtt atctcgcgcc tgaaccgtgc cggggtcgaa ctggtcgcca
    23101 gtggcgatat cagcctgcag ttcggcgata tcctcaatct ggtggggcgt ccgtccgcaa
    23161 ttgatgccgt tgccaatgtg ctggggaatg cgcagcaaaa actgcaacag gttcagatgc
    23221 tgccagtgtt tattggcatc gggcttggcg tattgttagg ttctattccc gtctttgtgc
    23281 ctgggttccc ggccgcgttg aaactggggc tggcgggcgg tccgctgatt atggcgttga
    23341 tcctcgggcg tatcggcagt atcggcaagc tgtactggtt tatgccgcca agtgccaacc
    23401 tcgcgctgcg ggagctgggg atcgtgctgt tcctctcggt cgttggtctg aaatctggtg
    23461 gagattttgt gaataccctg gtcaatggcg aagggctaag ctggattggt tatggtgccc
    23521 tgatcaccgc cgttccgctg attactgttg gcattctggc gcggatgtta gccaaaatga
    23581 attacctgac catgtgcggg atgctggcag gttccatgac cgatcctccg gcgctggcgt
    23641 ttgctaataa tcttcatcca accagcggtg cggcggcgct ctcttacgcc actgtctatc
    23701 cgttagtgat gttcctgcgc attatcaccc cccaattact ggcggtgctc ttctggagta
    23761 tcggttaact cttttccggg tggagtcgcc gtaaatggta ctccacctgg tattcgctgg
    23821 catttctgaa catcactgaa taattcaaaa actccccgct ctcgctatat gacaacgagg
    23881 tgatccgcag cagtggcgtg tgttccgcta ccttcatttg ccgcgccagc agtctatcgg
    23941 cgagcactgg cgtcaggctt tcataattac cgccaatcaa aatcccgcac tcttgttcaa
    24001 tatattcaaa cttcgaccct tccagatgtt gcagcgaaag attacggaac agttttaccg
    24061 gcatatagct gtcttccagc atcagcggtt tcccttccac aaaacgaacg cgacgggaga
    24121 agtagatctg ctcgttgatt tgaatccgta actggctggc aatggccgga ggcgcaggca
    24181 tgatttcaaa aatcagcacc tggctggtga ccgtttttcc ctgccgtttt aacacctcca
    24241 ccagcccggt caggctggcg gtttgatgca gtacatcttt gcgcaccagg taagtgccgc
    24301 tgccgtggcg gcggaccacc aatccccacg ctaccagcag gtcaatggct ttacggatgg
    24361 tcatccgcga taccgcaaat tcttccgcca actttttttc accgggaaga aggctattta
    24421 gcgtgaaatc tgcggaatta agtcgaattc ttaaccgctc cgcaatgctt ttgtagatca
    24481 tctctagatc tcttttttgc ctgcaagcac gggtaatgcg cccacaagtt ctgcttaatc
    24541 gattgaaaaa acatcttttt tttaaagatg tgttcgatgc tgtgtacctg tgctcacaga
    24601 tgtctacttt ttcgcgaaaa cgtagatctc taccgcccaa cgaaaagcat gaaagcgatc
    24661 acgaatccca ttgggtcgtc atgttctgcc gatcgcatct tcctatcctc gctccaggcc
    24721 tgccgcataa ccaatcaggc ttcctactta cagaattgag aaaagaggat gtggaaatgc
    24781 tcagtcaaat tcaacgcttt ggcggcgcga tgttcacgcc agtgctgctg tttcccttcg
    24841 ccgggattgt ggtgggtctt gccatcttgc tgcaaaaccc gatgtttgtc ggggaatcac
    24901 tgaccgatcc gaacagttta ttcgcgcaaa tcgtacacat tattgaagag ggcggttgga
    24961 cggtgttccg taatatgccg ctgatttttg ctgtcggttt acccattggc cttgctaagc
    25021 aagcgcaggg gcgtgcttgt ctggcggtga tggtgagttt cctgacctgg aactatttca
    25081 tcaacgcgat gggaatgacc tggggaagct acttcggcgt cgatttcact caggacgcgg
    25141 tggcaggtag cggtctgaca atgatggccg ggattaaaac cctcgatacc agcattatcg
    25201 gcgcaattat catttccggc attgtgacgg cgctgcataa ccgtctgttc gataaaaaac
    25261 tgccggtttt tctcggcatt ttccagggga cgtcttatgt ggtgattatc gccttcctgg
    25321 tgatgatccc ctgtgcctgg ctgacgttgc tcggctggcc aaaagtacaa atggggattg
    25381 aatctctgca agcgttcctg cgttcggcgg gtgcgcttgg ggtgtgggtt tacaccttcc
    25441 tcgaacgtat tctgatccca accggtttac accacttcat ctacggaccg tttatctttg
    25501 gtccggcagc tgttgaaggc ggcattcaga tgtactgggc gcagcatctg caagagttca
    25561 gtctgagcgc cgagccgctg aaatcgttgt tcccggaagg cggttttgcc ctgcacggta
    25621 actcaaaaat ctttggtgcc gtgggcattt ctttagcgat gtacttcact gccgcaccgg
    25681 aaaatcgggt aaaagtggcg ggcttgctga ttcccgcaac cttaaccgcc atgctggtgg
    25741 gaattaccga accgctggaa tttaccttcc tgttcatttc accgttgctg tttgcggtac
    25801 acgccgtgct ggcggcctca atgtcgaccg tgatgtatct ctttggtgtg gtgggcaaca
    25861 tgggcggagg tctgattgac caggttttac cgcaaaactg gatcccgatg ttcagcaacc
    25921 acgcggatat gatgctgacc caaatcgcca ttgggttgtg ctttaccctg ctgtacttcg
    25981 tggttttccg caccctgatt ctgcagttca acatgtgcac gccgggacgt gaagatgcgg
    26041 aagtgaaact ctactcaaaa gccgaataca aagcctcgcg aggccaaacc accgcggcag
    26101 agccaaaaaa agagctggat caggctgccg gtattctgca agccctgggc ggggtcggca
    26161 atatctccag cattaacaat tgcgcgacgc gtctacgcat tgcactgcat gacatgtcac
    26221 aaacgctgga tgacgaagtc tttaaaaagc tgggagcgca cggcgtcttc cgtagtggcg
    26281 atgccattca ggtgatcatt ggtctgcatg tatcccaatt gcgtgaacag ctcgatagct
    26341 taattaattc tcatcaatca gcagaaaatg ttgccattac ggaggcagta taatgaccaa
    26401 attctcagtg gttgtcgcag gcggtggaag cacctttacg ccaggcatcg tgttgatgct
    26461 cctggcgaat caggaccgtt tcccgcttcg tgcactgaaa ttttatgata acgatggcgc
    26521 gcggcaggaa gtgattgccg aagcctgtaa agtcatcctt aaagaaaaag cgccggacat
    26581 tgcgtttagt tacaccaccg atcctgaagt agcgttcagc gacgttgatt ttgtcatggc
    26641 gcacatccgc gtcggcaagt acccgatgcg cgaactggat gaaaaaatcc cactgcgcca
    26701 cggtgttgtt ggtcaggaaa cttgcggacc cggcggaata gcgtacggca tgcgttccat
    26761 tggcggcgtc ctggaactgg tggattatat ggaaaaatat tcaccaaacg cctggatgct
    26821 caactactcc aacccggcag caattgtcgc agaagccacg cgtcgtctgc gcccgaatgc
    26881 gaaaatcctc aacatctgtg acatgccaat cggtattgaa agccggatgg cgcaaattgt
    26941 tgggctgcaa gatcgcaaac agatgcgcgt gcgctactac ggcctgaacc actggtggtc
    27001 ggcgatatcc cgcagtttca gaaagggtta atgagtcagc aagtggcggt ggaaaaactg
    27061 gtcgtggacg cctgggaaca gcgttcatat cagcacctgt ggcaggcgat tacgttgtcg
    27121 aaaacggtac cgagcgcctc ggtcgccaaa gctattctgg atgagttgct ggaggccaac
    27181 aaagcgtact ggccagagtt acgttaattg tgtggactgg catccgttgg gtgctggtcc
    27241 gtattcttta gcacggctga cggcgcataa ccaaatttat gtttaaaggc tttgctgaaa
    27301 tgaaaggagt cgaagaagtt gagcgtatca gcgacttcgc caaccgagtt tccttgttgc
    27361 tttaatagcg atagcgccag atccagacgc gcatcgaggt aatattcttt cggcgtcttg
    27421 ccggtatagc gtaaaaacag acgacgcaac caggcttcgc tacaggggat cgtggcagcc
    27481 atatcagcta cgctccagcg ttgttgcaga ctggcatgta aagtggcaat taatttttca
    27541 atttgccgcc gttgtggatc ttttttacca tctgcgtaca taagacaaat ccactgataa
    27601 ataattttcg tcagaaaagc gactgccaga ttatttttta ttgcttctgg tgaagttatt
    27661 aactcagcaa cttcggtgag ttcctgatta taaatttcgc cattataaat aacgctttgc
    27721 tgaccaacgg gaatatccat catactggtg ggggtaaatt ccatccagta ctgttcccag
    27781 actaaacctt cacagtgata agagtgaata tccattggct ttaagaatat aatacagttg
    27841 ccatgcagtg ttatttgttc gccattcttt aaaagaattt tcccacatcc ttgtacggta
    27901 tacaccgcaa cccatgagtt ggcgataagc ggtttcttct tgtcccgcgg ccagatcacc
    27961 cggtaatgct cgtcggcgta ggtatgccag agcgagatca gagaaattcc gctggagatc
    28021 acattttcgt taatcaataa gggaatattg tacggagttt cgttttttac atctgaactt
    28081 tgcaattttc cattcatttt aatatccctc cggcgaatta tgctcacaac ctgaacttaa
    28141 cagaaaacaa tccgttcagg gatacgacgt ttgtatttta agaatctgac tgcctgaccc
    28201 gacgcatttt acctctccct atattcatgc gtccgggaca taaacataaa taagggctat
    28261 gagatgaatt cgttacaaat cttgagtttt gtcggtttta cgctgctggt ggcggtgatc
    28321 acctggtgga aggtccgcaa aacagatacc ggatcgcaac aaggctattt tcttgccgga
    28381 cgttcactaa aagcgccggt tattgccgct tcgttaatgc taaccaacct ttccacggaa
    28441 caactggtcg gcctttccgg gcaggcctac aaaagcggca tgtcggtgat gggctgggaa
    28501 gtgacttcag cggtgacgct gatcttcctc gcgctaatct ttttaccgcg ctatctgaag
    28561 cgcggcattg ccaccatccc cgattttctg gaggaacgtt atgataaaac gacgcgtatt
    28621 atcatcgact tctgcttcct cattgccacc ggcgtctgct ttctgccgat tgttctctac
    28681 tccggcgcgt tggcgctcaa cagcctgttt cacgtcgggg aatcgctaca gatttctcac
    28741 ggtgcggcta tctggctact ggtaattttg cttggtctgg cgggaatttt gtatgcggtg
    28801 atcggcggac tgcgcgcaat ggcagtggcg gactccatca acggtattgg gctggttatc
    28861 ggcgggttga tggtgccggt atttggcctg atcgcgatgg gcaagggcag ctttatgcaa
    28921 ggcattgagc aactcaccac cgttcacgcc gagaaattaa actcaatcgg tggcccgacc
    28981 gatcccttgc cgattggcgc ggcatttacc ggtttgattc tggtgaacac cttttactgg
    29041 tgtacaaatc agggcatcgt gcaacgcacg ctggcgtcaa aaagcctggc ggaagggcaa
    29101 aagggggcgc tgttaacggc ggtgctgaaa atgctcgacc cgctggtact ggtgctgcca
    29161 gggttgattg cgtttcatct gtatcaggat ttaccgaaag ccgacatggc ctacccgacg
    29221 ctggtcaata acgttctgcc agtgccaatg gtgggtttct tcggcgcggt gttatttggt
    29281 gcggtgatca gtaccttcaa cggctttctg aatagcgcca gtacgttatt cagtatgggt
    29341 atttaccgtc gcatcattaa ccagaatgcc gagccgcagc agctggtcac cgtcgggcgc
    29401 aaatttggtt tctttatcgc tatcgtttcg gtgctggtcg cgccgtggat cgccaacgcg
    29461 ccgcaggggc tgtatagctg gatgaaacag ctcaacggca tttacaacgt gccgctggtt
    29521 accatcatca ttatgggctt tttcttcccg cgcatcccgg cgctggcggc aaaagtggcg
    29581 atggggattg gcataatcag ctacatcacc atcaactatc tggtgaagtt cgacttccat
    29641 ttcctctatg tgctggcctg tacgttctgc atcaacgtgg tcgtgatgct ggtgatcggt
    29701 tttatcaaac cgcgcgccac gccgttcacc ttcaaagatg cgtttgcggt ggacatgaaa
    29761 ccgtggagaa acgtcaagat cgcgtcaatt ggcatcctgt tcgcgatgat tggcgtctat
    29821 gccgggctgg ctgaattcgg cggctacggt acgcgctggt tagcgatgat cagttatttc
    29881 attgccgccg tagtgattgt ctacctgatt tttgacagct ggcggcatcg tcacgaccca
    29941 gccgtaacct ttactcccga cgcgaaggat agcctatgaa acgccccaat tttctgttca
    30001 tcatgaccga tacccaggcc accaatatgg tcggttgcta tagcggtaaa ccgctgaata
    30061 cgcaaaatat tgatagtctg gcggcggaag gtattcgctt taattccgcc tacacctgtt
    30121 caccggtttg tacgcctgca cgcgccggac tatttaccgg tatctacgct aaccagtccg
    30181 gcccgtggac caacaacgtc gcgccaggca aaaacatctc cactatgggg cgctacttta
    30241 aggatgccgg ctatcacacc tgttacatcg gcaaatggca tctcgacggt catgactatt
    30301 tcggcactgg cgagtgtccg ccggagtggg acgctgatta ctggttcgat ggggcgaact
    30361 accttagcga actgacggaa aaagagatca gcctgtggcg caatggccta aacagcgttg
    30421 aggatttaca ggcgaaccat atcgacgaaa ccttcacctg ggcgcaccgc atcagcaatc
    30481 gggcagtaga ttttctgcaa cagcccgcgc gcgccgagga acccttcctg atggtggttt
    30541 cgtatgatga gccgcatcac ccgttcacct gtccggtgga gtatttagag aaatacgctg
    30601 atttttacta cgatctgggc gagaaagctc aggatggcct ggcgaacaaa ccggaacatc
    30661 accgcttatg ggcgcaggcg atgccatcgc cagtcggtga tgacgggctt tatcaccatc
    30721 cgctctattt tgcctgtaat gactttgttg atgaccaaat cggacgggtc atcaatgcct
    30781 taacgccaga gcaacgtgaa aatacgtggg tcatttatac ttccgatcac ggcgaaatga
    30841 tgggcgcaca taagctgatc agtaaaggag cggcgatgta tgacgacatc acccgtattc
    30901 cgctgatcat ccgttcgccg caaggggagc ggcgacaggt cgatacgcca gtcagtcata
    30961 tcgatttact gccgacaatg atggcgctgg cagatattga aaaaccagag attctgccgg
    31021 gggaaaatat ccttgccgtg aaagagccac gcggtgtaat ggtggaattt aaccgctacg
    31081 agattgagca tgacagcttt ggcggtttta ttccggtgcg ttgctgggtg acggatgact
    31141 ttaaactggt actcaacctc ttcaccagtg atgaacttta cgatcgccgt aatgacccaa
    31201 atgaaatgca taacctgatc gatgatatcc gttttgcaga cgttcgcagc aaaatgcatg
    31261 acgccttatt ggattacatg gacaaaattc gcgatccgtt ccgcagttac caatggagcc
    31321 tgcgtccgtg gcgtaaagat gcactgccgc gctggatggg ggcatttcgt ccacgcccac
    31381 aagatggcta ttcgccggtg gtacgtgact atgacaccgg cctaccgacg caaggagtga
    31441 aagtggaaga gaaaaaacag aagttctgac gataatgccc gctgatgaac acccggcggg
    31501 cattattcag gctgcttaaa ccagtgaacc gccggtcagg aaggaaaaaa tcaggaatat
    31561 cagcaaaatg ctgcacacga tgccgatacc aaaaagcaag gtatggaaag ccagcgtccc
    31621 gccgccaaaa accagcgcgc cgcgaatgcc ccagcgattt tccttacgca taccgccaat
    31681 aaatgcgcaa agtagcgcag caatggccag tgcgataccg ctttgatcga ggattttatc
    31741 gacatctacg tcctcttttt tctccacggt ggtaattttt tcgcctttta ttccggcgag
    31801 aagacctctt ttaatttcag ccgttttgtc cgccacgatg ctttcaaacg ttggcggtgg
    31861 tgcggaaaaa gggccgaatg aaaagtggat aatccccatc attaaagcga tggcaccgaa
    31921 cagcatgccc aatgaactga ttttattttg agctatgata cccatctgac ctccttgtag
    31981 cggatggcga aagcatatca ggaaacgggc ggacgaaatt cttaaaaaaa aaccgctaaa
    32041 ggtagagcgt tagtgaaatt gcaaaggagc aacaatgaaa atttcccgcc ttggagaagc
    32101 gcccgattac cgcttctcgc tggctaatga gcgtactttt ctggcgtgga tccgtaccgc
    32161 actaggattt ctggcggcgg gggttgggct tgatcaactt gcgccagatt tcgccacgcc
    32221 cgtcattcgt gaactgctgg cgttgttgtt gtgtctgttt tccggcgggt tagcgatgta
    32281 tggctacctg cgctggttac gcaatgaaaa agcgatgcgt ctgaaagagg acctgcccta
    32341 caccaacagc ttattaatca ttagcttaat attgatggtc gtggcggtga tcgttatggg
    32401 actggtgttg tatgccggat agccgcaaag ccagacgcat tgccgaccca ggactgcagc
    32461 cggagcgcac atcactggcg tggtttcgta ccatgctggg ctacggcgcg ttgatggcgt
    32521 tggctatcaa acacaactgg caccaggcgg gcatgttatt ctggatttcg attggcattc
    32581 tcgccatcgt ggcgctgatc ctctggcact acacccgtaa tcgcaattta atggatgtca
    32641 cgaatagcga tttttcccaa tttcacgtag tccgtgacaa atttttgatc tccctcgcgg
    32701 tgttatctct cgcaatactg tttgctgtaa cgcatataca tcaacttatc gtatttattg
    32761 agagagtcgc atgacaggaa gtcaggtcat tgatgctgaa gaggacaggc ataaactggt
    32821 tgtagagtac aaggatgctc tgcaacctgc cgatttttat cacaatttca aacagcgggg
    32881 catccgctcc gtacagctca ttccctatct tgaatttgat gaccggggcg atctgacggc
    32941 cgcttcggtg acagcagagc tgtgggggaa atttttaatt gccctgttcg agtgttgggt
    33001 gcgggcagat atcagtcgta tctcgattga gctttttgac gccaccttac aaaaatggtg
    33061 tggcagcgaa aacccgcaac cgcgccgtga ctgccaggcg tgcgactggc atcgcctgtg
    33121 cccgcacgct cgccaggaga cgccagacag cgtgctttgt gcgggctatc aggcgtttta
    33181 ttcttactcc gcaccgcata tgcgggtgat gcgtgatctg ataaagcagc atcgttctcc
    33241 catggagctg atgacgatgc tgcggtgacg tgcgcttaaa cgggctgccc ctgatgcgac
    33301 atccgcgtcg ccagcggcag ccagcacagc acgatcaaca atcccattaa ggtcatcaac
    33361 aaccccaggc tgccctgacc ggtttgcggc aacatcgcag agagcgacgc cagcacgccg
    33421 gaaccaatgt tttgcagacc gccgaccagc gcgccagccg tgcccgccag gaaggggaac
    33481 ggctccatcg cgccgctggt cgccagcgga aacagcatcc cggcaccgaa aaagaacagc
    33541 gcggcgggaa cgagcagcgt ccagacattc atcacgccaa accagtcggg gatccacatc
    33601 agcaagccag ccagcaggca gcagataacc gactgccaca ttaacgtgga gaagcgttta
    33661 ttgggacgtc cggcaaacca tgcgccaaaa aacgctgccg gaatcggcag aataaacaaa
    33721 atactgaccg tcatactgct cagccctaac accgcgccca tcagcacgcc ggagcaggct
    33781 tcaaaggcgg caatcccggc cagaccgcca atcagcatca gcaaataaca gttaaaaccg
    33841 ctgttaccga aaagcgtttt ataactggta agcaggcgcg tgcgcggtgc atcgaccgga
    33901 cgcgtttccg gcatccagcg ggccatactg aaggtcacac cagcacaaag aaccaacaaa
    33961 aagagataac aggcgcgcca gttccacatc gtatccagca gaccgccgat tagcggtgcg
    34021 agcaacggac tgacgagaat ccccatgttt aacaggctgt tagcatggcg caactgtgtc
    34081 cgttcatata aatctcgcgg caaagtacgc gccattacgc cgccaacgcc ggtgcccatc
    34141 ccctgcatcg cgctggcggc aatcaacacc gtcaaactgg aggtcgtgac cgcgaccagc
    34201 gttgccagca taaaaatgga cattccgacg aggatcaccg gtcggcggcc cacgcggtcg
    34261 gaaatcgggc cataaaacag ctgtgagaca ccgtaagtca gcagataagc gcccattacg
    34321 ctctgcaccg ccccttcacg gacgttgaga tcgcgcgcca tatcggcaat agctggaata
    34381 taaatggttt gcgccatctg accgacggcc acgagtaata ccaacatcaa taacaaattg
    34441 acgtttcttt gccttttcat tatcacggat gcttttataa aaataattag aataagtcac
    34501 tacgcattcc cataaatgcg ggagaggaat ctatcacatt gatgaatgtt agccagatat
    34561 acgccaggaa tggggaattg tttagcggca gaatatgtaa acaaaagcgg caataaatgt
    34621 tgccgggagg gattacgtgc gaactgtaac cctctcccca aaggggcgag ggggaaacca
    34681 ccgttccctt tcagaaaatg agctaaacat tgcacgggac ggtcccctcg cccctttggg
    34741 gagagggcta gggtgagggg cacaggcagc gcaggtgcaa acattctgct ggtagaagaa
    34801 gcccccaact ccaacccgca cgctaaatat aaaaggggag cggttacccg ctcccctttg
    34861 gtgcgacttg aatctgaatt acttcaggta tttcagaaca gcatcaagca gttgcagtgc
    34921 tgcaacaatg agtttgagga taagaatggc gatatccacc aggctcatac gcgtctcctg
    34981 tggttcagga gcactgccag ctgacgtacc tttccgctgc ctgttgccag ctgttgtgca
    35041 cgcggtatct taaggagaaa cgttgtgctt gttgacataa cacagtgtgc tcacgcgggg
    35101 ttaaggcgct gacggcacca cccgtttcag ccaggactta gtgcgccggg taacgatgcg
    35161 gcccccgcat aacacattgc gtacagtgat attatagaag tttactgtat aaataaacag
    35221 taatatttgg acaaaacgca aactgtgatc gatactcaac tatgccctaa atacgtcaaa
    35281 attcgtgccg aaattgcgcg ttctgcgcgg aacacgtata ctttcagtgt tgacataata
    35341 cagtgtgctt tgcggttacc agccgcaggc ggctgatgaa acctcgctcc ggcggggttt
    35401 tttgttacct gcaattcagt acaaaacgtg atcagcccct caattttccc ttgctgaaaa
    35461 attttccatt gtctcccctg taaagctgtg cttgtataaa tattgttaaa cacaaaacca
    35521 acaaggtccc caatgactac ttccatgttc aacgcaaaac tactaccaac tgcgctatcc
    35581 gccgcagtgg tcgtcgtgcg tgtggtggtg gtcgtcggca atgcgccgta gggactggaa
    35641 caacacacga ttccaaaacc ccgccggcgc aaaccgggcg gggtttttcg tttaagcacc
    35701 tcccggaaag tcggcccaga agaaaaggac tggagcatgg caagttcggg cacaacatcg
    35761 acgcgtaagc gctttaccgg cgcagaattt atcgttcatt tcctggaaca gcagggcatt
    35821 aagattgtga cgggcattcc gggcggttct atcctgcctg tttacgatgc cttaagccaa
    35881 agcacgcaaa tccgccatat tctggcccgt catgaacagg gcgcgggatt tatcgctcag
    35941 gggatggcgc gcaccgacgg taaaccggcg gtctgtatgg cctgtagcgg accgggtgcg
    36001 actaacctgg tgaccgccat tgccgatgcg cggctggact ccatcccgct gatttgcatc
    36061 accggtcagg ttcccgcctc gatgatcggc accgacgtct tccaggaagt ggacacctac
    36121 ggcatctcta tccccatcac caaacacaac tatctggtca gacatatcga agaactcccg
    36181 caggtcatga gcgatgcctt ccgcattgcg caatcaggcc gcccaggccc ggtgtggata
    36241 gacattccta aggatgtgca aacggcagtt tttgagattg aaacacagcc cgctatggca
    36301 gaaaaagccg ccgcccccgc ctttagcgaa gaaagcattc gtgacgcagc ggcgatgatt
    36361 aacgctgcca aacgcccggt gctttatctg ggcggcggtg tgatcaatgc gcccgcacgg
    36421 gtgcgtgaac tggcggagaa agcgcaactg cctaccacca tgactttaat ggcgctgggc
    36481 atgttgccaa aagcgcatcc gttgtcgctg ggtatgctgg ggatgcacgg cgtgcgcagc
    36541 accaactata ttttgcagga ggcggatttg ttgatagtgc tcggtgcgcg ttttgatgac
    36601 cgggcgattg gcaaaaccga gcagttctgt ccgaatgcca aaattattca tgtcgatatc
    36661 gatcgcgcag agctgggtaa aatcaagcag ccgcatgtgg cgattcaggc ggatgttgat
    36721 gacgtgctgg cgcaattgat cccgcaggtg gaagcgcaac cgcgtgcaga gtggcaccag
    36781 ttggtagcgg atttgcagcg cgaattcccg tgtccaatcc cgaaagcgtg cgatccatta
    36841 agccattacg gcctgatcaa cgccgttgcc gcctgtgtcg atgacaatgc gattatcacc
    36901 accgatgtgg gtcagcatca gatgtggacc gcgcaagctt atccgctcaa tcgcccacgc
    36961 cagtggctga cctccggtgg gctgggcacg atgggttttg gcctgcctgc ggcgattggc
    37021 gcggcgctgg cgaacccgga tcgcaaagtg ttgtgtttct ccggcgacgg cagcctgatg
    37081 atgaatattc aggagatggc gaccgccagt gaaaatcagc tggatgtcaa aatcattctg
    37141 atgaacaacg aagcgctggg gctggtgcat cagcaacaga gtctgttcta cgagcaaggc
    37201 gtttttgccg ccacctatcc gggaaaaatc aactttatgc agattgccgc cggattcggc
    37261 ctcgaaacct gtgatttgaa taacgaagcc gatccgcagg ctgcattgca ggaaatcatc
    37321 aatcgccctg gcccggcgct gatccatgtg cgcattgatg ccgaagaaaa agtttacccg
    37381 atggtgccgc caggtgcggc gaatactgaa atggtggggg aataagccat gcaaaacaca
    37441 actcatgaca acgtgattct ggagctcacc gttcgcaacc atccgggcgt aatgacccac
    37501 gtttgtggcc tttttgcccg ccgcgctttt aacgttgaag gcattctttg tctgccgatt
    37561 caggacagcg acaaaagcca tatctggcta ctggtcaatg acgaccagcg tctggagcag
    37621 atgataagcc aaatcgataa gctggaagat gtggtgaaag tgcagcgtaa tcagtccgat
    37681 ccgactatgt ttaacaagat tgcggtgttt tttcagtaac cgctcaaggc ttgaacaaca
    37741 tcgcgcttat cgttaaggta agcgcgtttt tttttacccg ccaggacaag accatgatca
    37801 ccgttgccct tatagacgat cacctcatcg tccgctccgg ctttgcgcag ctgctggggc
    37861 tggaacctga tttgcaggta gttgccgagt ttggttcggg gcgcgaggcg ctggcggggc
    37921 tgccggggcg cggtgtgcag gtgtgtattt gcgatatctc catgcccgat atctccggtc
    37981 tggaactgct aagccagctg ccgaaaggta tggcgacaat aatgctctct gttcatgaca
    38041 gtccggcgct ggttgagcag gcgcttaacg cgggggcacg cggctttctc tccaaacgct
    38101 gtagccctga cgaactgatt gcggcggtgc atacggttgc cacgggcggc tgttatctga
    38161 cgccggatat tgccattaaa ctggcatccg gtcgtcagga cccgctaacc aaacgcgaac
    38221 ggcaggtggc ggaaaaactg gcgcaaggaa tggcggtgaa agagattgct gccgaactgg
    38281 gcttgtcgcc gaaaacggtg cacgtccatc gcgccaacct gatggaaaaa ctgggtgtca
    38341 gtaacgacgt agagctggcg cgccgcatgt ttgatggctg gtgatgaaga cgttgttctc
    38401 ccgcttaatt accgttattg cctgcttttt tatcttctct gccgcatggt tttgcctgtg
    38461 gagtatcagc ctgcacctgg ttgagcgccc tgatatggcg gtgctgttat ttccgtttgg
    38521 tctgcgtctg gggctaatgc tgcaatgccc gcgcggatac tggccggtgc tgctgggcgc
    38581 ggagtggctg ctgatttact ggctaacgca ggcggtcggt ttaacccatt ttccgttatt
    38641 gatgatcggt agtttactga cgttactgcc cgtggcgctg atctcgcgct atcgccatca
    38701 gcgtgactgg cgcaccttgc tgttacaggg tgcggcgtta acggcggcgg cgttgttgca
    38761 gtcgctgccc tggctttggc acggcaaaga gtcgtggaat gcgctgttgc tgactttaac
    38821 tggcggcctg acgctggccc cgatatgtct ggtgttctgg cactatctcg ccaataacac
    38881 ctggctgccg ctcggcccgt cactggtttc tcagccgatc aactggcgcg ggcgacatct
    38941 ggtctggtac ttgctgctgt ttgttatcag tctctggctc cagttgggat tgccggacga
    39001 actgtcgcgc tttacgccat tttgtctggc gctgccgatt atcgcgctgg cctggcacta
    39061 tggttggcaa ggggcgctga ttgcgacgtt gatgaacgcc atcgcgctga tcgccagtca
    39121 aacctggcgc gatcatccgg tggatttatt gctctcgctg ctggtgcaaa gtctgacagg
    39181 gttgttgctt ggcgctggca tccagcggtt gcgtgaactt aaccagtcgc tgcaaaagga
    39241 actggcgcgc aatcagcatc tggctgaacg gttgctggaa accgaagaga gcgtgcgccg
    39301 tgatgtggcg cgtgagctgc atgatgatat cggtcagacc atcacagcca ttcgtactca
    39361 ggcgggcatt gttcagcggc tggcggcaga taacgccagc gtgaagcaga gcgggcagct
    39421 catcgaacaa ctatcgctgg gcgtttacga cgcggtgcgc cgtttgttgg gtcggttacg
    39481 tccgcgccag ttggatgatc tcaccctgga gcaggccatc cgctcactga tgcgggaaat
    39541 ggagctggaa gggcgcggta ttgtcagcca tctcgaatgg cgaatcgatg aatcagcgtt
    39601 aagcgaaaac cagcgcgtga cgctgtttcg tgtctgccag gaagggctga acaacattgt
    39661 gaaacatgct gatgccagcg cggtcaccct gcaaggctgg cagcaggatg aacggttgat
    39721 gctggttatt gaagacgatg gcagcggttt gccgccgggt tccgggcaac aaggttttgg
    39781 cctcaccgga atgcgcgagc gcgtaacggc gctgggtggc acattacaca tttcctgtct
    39841 gcacggcacg cgtgtcagcg tttctctacc tcaacgctat gtctaaggtt tgatgatgtt
    39901 gccgtttctg aaagcgcctg ccgatgcgcc attaatgact gataaatatg aaattgatgc
    39961 ccgctatcgc tactggcgtc ggcatattct gctgaccatc tggctgggtt acgcgctgtt
    40021 ttacttcacg cggaaaagtt ttaacgccgc ggtaccagaa atccttgcta acggcgtgct
    40081 cagccgtagc gatatcggcc tgttagcgac cctgttttac attacttacg gcgtgtcgaa
    40141 gtttgtctcc ggcattgtca gcgatcgctc aaatgcccgt tattttatgg ggatagggct
    40201 tatcgccacg ggcatcatga acattctgtt tggcttctcg acgtcgctat gggcgtttgc
    40261 cgtgctctgg gtgctgaacg cctttttcca gggctggggt tcaccggtgt gtgcgcgtct
    40321 gttaacggcc tggtattcac gtaccgagcg cggcggttgg tgggcattat ggaacacggc
    40381 gcataacgtc ggcggcgcac tcattcccat tgtgatggca gcgagcgcgc tgcattacgg
    40441 ctggcgtgcc gggatgatga ttgctggttg tatggcgata gtcgtgggga tttttctctg
    40501 ctggcggcta cgcgatcgcc cgcaggcgtt aggtttaccg gcggtcggtg aatggcggca
    40561 cgacgcgctg gaaattgctc aacaacaaga aggggcaggg ttgacgcgta aagagatcct
    40621 gaccaaatat gtgttgctga atccgtatat ctggctgctt tcgttttgct atgtgctggt
    40681 ctatgtggtc cgggcggcaa tcaacgactg gggcaatttg tatatgtccg agacgctggg
    40741 cgtcgatctg gtcacggcga atacggcagt gacgatgttt gaactgggcg gatttatcgg
    40801 tgcgctggta gccggttggg gctcggacaa attgtttaac ggcaaccgtg ggccgatgaa
    40861 tttgattttc gccgccggaa ttttgctttc agtcggctcg ctgtggctga tgccatttgc
    40921 cagctacgtg atgcaggcaa cctgcttctt caccattggt ttttttgtct ttggcccgca
    40981 gatgttaatc ggcatggcgg cggcagagtg ttcccacaaa gaggctgcag gggcggcgac
    41041 ggggtttgtc ggcttgtttg cttatctggg ggcgtcgctt gctggttggc cgctggcgaa
    41101 agtactcgat acctggcact ggagcggatt ttttgtggtt atctctatcg ccgccggggt
    41161 ttccgcactg ctgttactgc cctttttgaa cgcccaggca ccgcgcgaag cgtgatgcat
    41221 ctcacctttt cacttcatat ccggcaaaac taagaaattt tccaggtttt gcctggacgc
    41281 tatctcaggc ctgatttgct gctgattttt acaatgccat cattcattaa ttctttccca
    41341 aaataattca agttgcagga acgcagcaaa cgcgtgagtc ccgggagcat agtgaactat
    41401 gtgaccggaa ggaatgcgtg cagctaaggc acctgcggct tgaagtatga agggaaatca
    41461 ggagtaaccc atgctggctt tcttaaacca ggttcgcaag ccgaccctgg accttccgct
    41521 cgaagtgcgg cgcaaaatgt ggttcaaacc gttcatgcaa tcctacctgg tggtctttat
    41581 cggctacctg acgatgtacc tgattcgcaa gaactttaac atcgcgcaga acgacatgat
    41641 ctcgacctac gggttgagca tgacgcagct ggggatgatc ggcctgggtt tctccatcac
    41701 ttatggcgtg ggtaaaacgc tggtttccta ctacgccgac ggcaaaaaca ccaaacaatt
    41761 cctgccgttc atgctgatcc tgtctgccat ttgtatgctg ggcttcagtg ccagtatggg
    41821 cagcggctcg gttagcctgt tcctgatgat tgccttctac gccttaagcg gctttttcca
    41881 gagtaccggc ggttcgtgca gttactccac catcaccaaa tggacgccgc gtcgtaaacg
    41941 cgggacattc ctcggtttct ggaatatttc tcacaacctt ggcggtgcag gcgcagcagg
    42001 tgtggcgctg ttcggggcaa attacctgtt cgatggccat gtcatcggca tgtttatctt
    42061 cccgtcgatt atcgcgctga ttgtcggttt tatcggcctg cgttacggca gcgactcccc
    42121 ggaatcttat ggcctcggca aagctgaaga actgttcggc gaggagatca gcgaagagga
    42181 caaagagaca gaatctaccg atatgaccaa gtggcagatc tttgttgagt atgtgctgaa
    42241 aaacaaagtg atctggctgc tgtgcttcgc caacattttc ctctatgtgg tacgtattgg
    42301 tatcgaccag tggtcaaccg tatacgcgtt ccaggaactg aaactctcta aagcggtggc
    42361 gattcagggc tttacgctgt ttgaagctgg tgcactggtc ggtacgctgc tgtggggctg
    42421 gctctctgac ctggcgaacg gtcgccgtgg cctggtggcc tgcatcgcgc tggcgctgat
    42481 tatcgccacg ctcggtgtgt atcaacatgc cagcaacgaa tatatctatc tggcttctct
    42541 ctttgcgttg ggtttcctgg tctttggccc gcaattgttg attggtgtgg ctgctgttgg
    42601 ctttgtacct aaaaaagcga ttggcgctgc cgatggtatt aaaggcacct ttgcttacct
    42661 gattggtgac agctttgcca agttaggtct gggaatgatt gccgatggga cgccggtatt
    42721 cggccttacc ggctgggcag gcaccttcgc cgcacttgat atcgccgcga ttggttgtat
    42781 ctgcctgatg gcgatagtgg cggtgatgga agaacgcaaa atccgccgcg agaaaaaaat
    42841 tcagcagttg acagtggcat aaacgtaact ggtgactttt gcccggcatg acgccgggct
    42901 tttttattat tccgtgactt ccagcgtagt gaaggcaaac ttctcgccat caaatagccc
    42961 ctgactggtt agttttagcg cggggatcac tggcagagaa agaaacgcca tctgaataaa
    43021 cggctcatcg ggtaacggac cgcattcacg ggcggcggct ttcaaggcgt caatttgttc
    43081 cgccagcgac tgcgccgtgt cggtgctcat caggccggca atgggcaacg gcagatgact
    43141 ttggacctgg ccgttacgta ccacgcacag cccgccgcca tcctgaatca cctgattgac
    43201 cgccagcgcc atctcttcgg cactgcgacc gatcaccaca atattatggc tgtcatggct
    43261 gaccgtcgca gccagcgcac cttcattcag tccaaagccg ccaagcaaac cacaagccgg
    43321 agccagccgt tgcccgtaac gctcaagtac ggcaataaag ctcacatcat cgcgatcaaa
    43381 accattttcg ctgtagacgc tggagtggga gtgcgtaatc aattcgttat ggatgacgtc
    43441 aatgacccga tagcgttttc cgggcgtaaa ttgcagggca aagtcgctgg cggaaactgg
    43501 ctggcgggca atggtgttgc catatggcgg agcggattgt gccagtctcg ccgactcttc
    43561 cgcctgtaag gtttgcgcat caatcggctc gcctttcacc agtacctgct gcaccgtgac
    43621 cttacgcgca tcgctcaaca ggacgatatc ggcctgcttg ccgggtgcca gtaagccgag
    43681 gtgattcaga ccaaagtggc gcgccgtcga ccagctggcg acgcgatatg ccacatgcag
    43741 cggcacattg tgttgttcga tcaggcggcg aattaaggca tcgatgtgtc cttcatgggc
    43801 gatctcccac gggttacggt catcggtaca gagcatgcat tgcgggctgt taaattcgtt
    43861 gatcaacggt gccagcgcgt tgagattgcg ggcagcggac ccttcgcgga tcatcaacga
    43921 catgccgagt tgtaatttcc ggcgtccttc ttccagctga taactttcgt ggcagttttc
    43981 aatacccgca gtaatatagg cgttaagttc tttaccaccc aaacccgggc agtgaccgtc
    44041 cagcgtcagg tggcgaaatg catccagttt atcgagcagc gcattctgcc cgctaattac
    44101 gccagggtag tccatcattt ctgcaaggcc ggtaacctgc ggatggtccc gccaggcgag
    44161 catctgttca agggtaaaac tggcaccgtt aacatcgcag ccttccaggg cgggtacgca
    44221 agagctgacc tgtaagtact ggttttgcct tgcctgttcg gcacagcggg caaaccaggc
    44281 gaatccggct tctcccatca cgttgacgat ttcatgaggg tcgcaaataa cggtcgtcag
    44341 gccgcgcggc agggtagcgg tttcaaaagt gaccggcgtc atcatgctgg attcaatatg
    44401 caggtgagca tcaataaacc ctggcaccgc cgttgcgccg cgagcatcaa tccgctgcaa
    44461 agccggagca tcagcgtatt ctgcgccaac accggcaatg taacgtcctt taatcacaat
    44521 tgggccggaa atttctccgc cattgatcag gtcgagaata gagacattat caataatata
    44581 atcggcaaca gcgtcgccac gggaaacggc taacaattcc tggtattcag cccgg
//
LOCUS       DAZGOB010000026        44285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGOB010000026 DAZGOB010000000
VERSION     DAZGOB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.026"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            146..649
                     /gene="ftnB"
                     /locus_tag="ACHLIF_001492"
     CDS             146..649
                     /gene="ftnB"
                     /locus_tag="ACHLIF_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO9605530.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(728..979)
                     /gene="yecJ"
                     /locus_tag="ACHLIF_001493"
     CDS             complement(728..979)
                     /gene="yecJ"
                     /locus_tag="ACHLIF_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO9605531.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMQAIQKAIALIELKFTPQGESH"
     gene            complement(1094..1180)
                     /gene="azuC"
                     /locus_tag="ACHLIF_001494"
     CDS             complement(1094..1180)
                     /gene="azuC"
                     /locus_tag="ACHLIF_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO9605532.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1443..1766
                     /gene="yecR"
                     /locus_tag="ACHLIF_001495"
     CDS             1443..1766
                     /gene="yecR"
                     /locus_tag="ACHLIF_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO9605533.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1937..2434
                     /gene="ftnA"
                     /locus_tag="ACHLIF_001496"
     CDS             1937..2434
                     /gene="ftnA"
                     /locus_tag="ACHLIF_001496"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO9605534.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2472..2711)
                     /gene="yecH"
                     /locus_tag="ACHLIF_001497"
     CDS             complement(2472..2711)
                     /gene="yecH"
                     /locus_tag="ACHLIF_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO9605535.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2902..4113
                     /gene="tyrP"
                     /locus_tag="ACHLIF_001498"
     CDS             2902..4113
                     /gene="tyrP"
                     /locus_tag="ACHLIF_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO9605536.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4175..4840)
                     /gene="yecA"
                     /locus_tag="ACHLIF_001499"
     CDS             complement(4175..4840)
                     /gene="yecA"
                     /locus_tag="ACHLIF_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO9605537.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5036..5122)
                     /locus_tag="ACHLIF_001500"
     tRNA            complement(5036..5122)
                     /locus_tag="ACHLIF_001500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5086..5088),aa:Leu,seq:taa)
     gene            complement(5135..5208)
                     /locus_tag="ACHLIF_001501"
     tRNA            complement(5135..5208)
                     /locus_tag="ACHLIF_001501"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5174..5176),aa:Cys,seq:gca)
     gene            complement(5263..5338)
                     /locus_tag="ACHLIF_001502"
     tRNA            complement(5263..5338)
                     /locus_tag="ACHLIF_001502"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5303..5305),aa:Gly,seq:gcc)
     gene            complement(5490..6038)
                     /gene="pgsA"
                     /locus_tag="ACHLIF_001503"
     CDS             complement(5490..6038)
                     /gene="pgsA"
                     /locus_tag="ACHLIF_001503"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO9605538.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6095..7927)
                     /gene="uvrC"
                     /locus_tag="ACHLIF_001504"
     CDS             complement(6095..7927)
                     /gene="uvrC"
                     /locus_tag="ACHLIF_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO9605539.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7924..8580)
                     /gene="uvrY"
                     /locus_tag="ACHLIF_001505"
     CDS             complement(7924..8580)
                     /gene="uvrY"
                     /locus_tag="ACHLIF_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO9605540.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8722..8838
                     /locus_tag="ACHLIF_001506"
     CDS             8722..8838
                     /locus_tag="ACHLIF_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9605541.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            8876..9052
                     /gene="yecU"
                     /locus_tag="ACHLIF_001507"
     CDS             8876..9052
                     /gene="yecU"
                     /locus_tag="ACHLIF_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO9605542.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTLFTA
                     NFHCNMTRKINEYA"
     gene            9039..9263
                     /gene="yecF"
                     /locus_tag="ACHLIF_001508"
     CDS             9039..9263
                     /gene="yecF"
                     /locus_tag="ACHLIF_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO9605543.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9331..10053)
                     /gene="sdiA"
                     /locus_tag="ACHLIF_001509"
     CDS             complement(9331..10053)
                     /gene="sdiA"
                     /locus_tag="ACHLIF_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO9605544.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(10283..11035)
                     /gene="tcyN"
                     /locus_tag="ACHLIF_001510"
     CDS             complement(10283..11035)
                     /gene="tcyN"
                     /locus_tag="ACHLIF_001510"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO9605545.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(11032..11700)
                     /gene="tcyL"
                     /locus_tag="ACHLIF_001511"
     CDS             complement(11032..11700)
                     /gene="tcyL"
                     /locus_tag="ACHLIF_001511"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO9605546.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11715..12701)
                     /gene="dcyD"
                     /locus_tag="ACHLIF_001512"
     CDS             complement(11715..12701)
                     /gene="dcyD"
                     /locus_tag="ACHLIF_001512"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO9605547.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(12806..13606)
                     /gene="tcyJ"
                     /locus_tag="ACHLIF_001513"
     CDS             complement(12806..13606)
                     /gene="tcyJ"
                     /locus_tag="ACHLIF_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9605548.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(13694..14245)
                     /gene="fliZ"
                     /locus_tag="ACHLIF_001514"
     CDS             complement(13694..14245)
                     /gene="fliZ"
                     /locus_tag="ACHLIF_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO9605549.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(14291..15010)
                     /gene="fliA"
                     /locus_tag="ACHLIF_001515"
     CDS             complement(14291..15010)
                     /gene="fliA"
                     /locus_tag="ACHLIF_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO9605550.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(15175..16638)
                     /locus_tag="ACHLIF_001516"
     CDS             complement(15175..16638)
                     /locus_tag="ACHLIF_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO9605551.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRVRELTVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGDAYTVNVDSGAVKDTT
                     GNDIFVSAADGSLTTKSDTNIAGTGIDATALAAAAKNKAQNDKFTFNGVEFTTTTAAD
                     GNGNGVYSAEIDGKSVTFTVTDADKKASLITSETVYKNSAGLYTTTKVDNKAATLSDL
                     DLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNEDAAK
                     SLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRIEDAD
                     YATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            16893..18305
                     /gene="fliD"
                     /locus_tag="ACHLIF_001517"
     CDS             16893..18305
                     /gene="fliD"
                     /locus_tag="ACHLIF_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO9605552.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDSAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            18320..18730
                     /gene="fliS"
                     /locus_tag="ACHLIF_001518"
     CDS             18320..18730
                     /gene="fliS"
                     /locus_tag="ACHLIF_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO9605553.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            18730..19095
                     /gene="fliT"
                     /locus_tag="ACHLIF_001519"
     CDS             18730..19095
                     /gene="fliT"
                     /locus_tag="ACHLIF_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO9605554.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            19173..20660
                     /gene="amyA"
                     /locus_tag="ACHLIF_001520"
     CDS             19173..20660
                     /gene="amyA"
                     /locus_tag="ACHLIF_001520"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO9605555.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(20694..21107)
                     /gene="yedD"
                     /locus_tag="ACHLIF_001521"
     CDS             complement(20694..21107)
                     /gene="yedD"
                     /locus_tag="ACHLIF_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO9605556.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            21294..22499
                     /gene="yedE"
                     /locus_tag="ACHLIF_001522"
     CDS             21294..22499
                     /gene="yedE"
                     /locus_tag="ACHLIF_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO9605557.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            22496..22729
                     /gene="yedF"
                     /locus_tag="ACHLIF_001523"
     CDS             22496..22729
                     /gene="yedF"
                     /locus_tag="ACHLIF_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO9605558.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            22836..23504
                     /gene="yedK"
                     /locus_tag="ACHLIF_001524"
     CDS             22836..23504
                     /gene="yedK"
                     /locus_tag="ACHLIF_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO9605559.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNIKNQGAEL
                     IQPV"
     gene            complement(23899..24213)
                     /gene="fliE"
                     /locus_tag="ACHLIF_001525"
     CDS             complement(23899..24213)
                     /gene="fliE"
                     /locus_tag="ACHLIF_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO9605560.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHATLDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            24428..26086
                     /gene="fliF"
                     /locus_tag="ACHLIF_001526"
     CDS             24428..26086
                     /gene="fliF"
                     /locus_tag="ACHLIF_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO9605561.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            26079..27074
                     /gene="fliG"
                     /locus_tag="ACHLIF_001527"
     CDS             26079..27074
                     /gene="fliG"
                     /locus_tag="ACHLIF_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO9605562.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            27067..27753
                     /gene="fliH"
                     /locus_tag="ACHLIF_001528"
     CDS             27067..27753
                     /gene="fliH"
                     /locus_tag="ACHLIF_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO9605563.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQMPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            27753..29126
                     /gene="fliI"
                     /locus_tag="ACHLIF_001529"
     CDS             27753..29126
                     /gene="fliI"
                     /locus_tag="ACHLIF_001529"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO9605564.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            29145..29588
                     /gene="fliJ"
                     /locus_tag="ACHLIF_001530"
     CDS             29145..29588
                     /gene="fliJ"
                     /locus_tag="ACHLIF_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO9605565.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            29585..30712
                     /gene="fliK"
                     /locus_tag="ACHLIF_001531"
     CDS             29585..30712
                     /gene="fliK"
                     /locus_tag="ACHLIF_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO9605566.1"
                     /translation="MIRLAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAVPVLSAPLGSHEWQQSLSQHISLFTRQRQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            30817..31281
                     /gene="fliL"
                     /locus_tag="ACHLIF_001532"
     CDS             30817..31281
                     /gene="fliL"
                     /locus_tag="ACHLIF_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO9605567.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            31286..32290
                     /gene="fliM"
                     /locus_tag="ACHLIF_001533"
     CDS             31286..32290
                     /gene="fliM"
                     /locus_tag="ACHLIF_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO9605568.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            32287..32700
                     /gene="fliN"
                     /locus_tag="ACHLIF_001534"
     CDS             32287..32700
                     /gene="fliN"
                     /locus_tag="ACHLIF_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO9605569.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            32703..33068
                     /gene="fliO"
                     /locus_tag="ACHLIF_001535"
     CDS             32703..33068
                     /gene="fliO"
                     /locus_tag="ACHLIF_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO9605570.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            33068..33805
                     /gene="fliP"
                     /locus_tag="ACHLIF_001536"
     CDS             33068..33805
                     /gene="fliP"
                     /locus_tag="ACHLIF_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO9605571.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            33815..34084
                     /gene="fliQ"
                     /locus_tag="ACHLIF_001537"
     CDS             33815..34084
                     /gene="fliQ"
                     /locus_tag="ACHLIF_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO9605572.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            34093..34878
                     /gene="fliR"
                     /locus_tag="ACHLIF_001538"
     CDS             34093..34878
                     /gene="fliR"
                     /locus_tag="ACHLIF_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO9605573.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLILTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            35168..35791
                     /gene="rcsA"
                     /locus_tag="ACHLIF_001539"
     CDS             35168..35791
                     /gene="rcsA"
                     /locus_tag="ACHLIF_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO9605574.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(35835..36023)
                     /gene="dsrB"
                     /locus_tag="ACHLIF_001540"
     CDS             complement(35835..36023)
                     /gene="dsrB"
                     /locus_tag="ACHLIF_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO9605575.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            36186..36413
                     /gene="yodD"
                     /locus_tag="ACHLIF_001541"
     CDS             36186..36413
                     /gene="yodD"
                     /locus_tag="ACHLIF_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO9605576.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            36711..37526
                     /gene="yedP"
                     /locus_tag="ACHLIF_001542"
     CDS             36711..37526
                     /gene="yedP"
                     /locus_tag="ACHLIF_001542"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO9605577.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(37523..39217)
                     /gene="dgcQ"
                     /locus_tag="ACHLIF_001543"
     CDS             complement(37523..39217)
                     /gene="dgcQ"
                     /locus_tag="ACHLIF_001543"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO9605578.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLHWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(39455..39637)
                     /gene="yodC"
                     /locus_tag="ACHLIF_001544"
     CDS             complement(39455..39637)
                     /gene="yodC"
                     /locus_tag="ACHLIF_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO9605579.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(39716..40633)
                     /gene="yedI"
                     /locus_tag="ACHLIF_001545"
     CDS             complement(39716..40633)
                     /gene="yedI"
                     /locus_tag="ACHLIF_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO9605580.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVAGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            40806..41726
                     /gene="yedA"
                     /locus_tag="ACHLIF_001546"
     CDS             40806..41726
                     /gene="yedA"
                     /locus_tag="ACHLIF_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO9605581.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(41715..42185)
                     /gene="vsr"
                     /locus_tag="ACHLIF_001547"
     CDS             complement(41715..42185)
                     /gene="vsr"
                     /locus_tag="ACHLIF_001547"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO9605582.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(42166..43584)
                     /gene="dcm"
                     /locus_tag="ACHLIF_001548"
     CDS             complement(42166..43584)
                     /gene="dcm"
                     /locus_tag="ACHLIF_001548"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO9605583.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(43651..44088)
                     /locus_tag="ACHLIF_001549"
                     /pseudo
     CDS             complement(43651..44088)
                     /locus_tag="ACHLIF_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            44144..>44285
                     /locus_tag="ACHLIF_001550"
     CDS             44144..>44285
                     /locus_tag="ACHLIF_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9605584.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTA"
BASE COUNT        10887 a        11403 c        10988 g        11007 t
ORIGIN      
        1 gacgttattc ttatttcaga tcatcgtcag aattgactcc acgatcacat ttcggaccgg
       61 cagaaaggaa ttattctgca aacagtaatt atggtgtttt gatttatctt gcacctctcc
      121 acttctggat ataaggatat taggtatggc aaccgctgga atgcttctca aactcaactc
      181 tcaaatgaac cgcgagtttt acgcatccaa tctctacctt cacctgagta actggtgttc
      241 tgaacagagt ctgaacggca ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac
      301 ccaaatgatg cgcatgttta actttatgaa gagtgtcggc gctaccccca tcgttaaagc
      361 cattgatgtt cccggtgaaa aactgaactc tctggaagaa ctgttccaaa aaacgatgga
      421 agaatacgag caacgttcta gtacgttggc acagttagcc gatgaagcga aagaactgaa
      481 tgatgattca accgtcaatt tcctgcgcga tctggaaaaa gaacagcagc atgatggtct
      541 gttgctgcaa accattcttg atgaagtgcg cagtgcgaaa cttgcgggta tgtgccctgt
      601 gcagaccgac caacatgttc tgaatgtcgt gtcacaccag ctgcattgat catcatcggc
      661 gctaatgcat tgcgccgatg aaggttttga gaaaccgcag cctcatctgt ttgaagcagc
      721 ggttttttta atgggattca ccctgtgggg taaacttgag ttcaataagc gcgatggctt
      781 tttggattgc ctgcatggtg accgggtctg cggcggcggg atggttagta aagtcgatat
      841 tcttcagctg actggacatt ttttcacgaa cttcaacggg cgcgattaca tcgagaacat
      901 ccagaatttg tttgataacc aactggcaag caaccacatc agaaaccagt tcctgatcgg
      961 cattcagcgg ctgggacatc gtaaactcct gatagcattt tgaaagccgt tatagtagcg
     1021 gcttcacatc ttcagcgata gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg
     1081 agcaggtttt ttattatcgg aacatattgc ctggcggtac gtctttgaac gtcttgcaat
     1141 agttattgaa catacttttc aggattttgc gcagtttcat cgcggcactc cgaccatttg
     1201 ttatacaggt gttattctct ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt
     1261 tatgtgatac aaatcacata aatacccctt taatgttata aaaatgataa tcaaaaaaca
     1321 gcccccctat ttctgacacc tacagatggc aagaaatagc gcctgccagg cgtcttttcc
     1381 ggccattgtc gcagcgctgt aacgcgtaaa atagtgcttt ctcttactct tctggctgga
     1441 ccatgagact tctgattctg actctttcat taataacgct ggcggggtgt acggttactc
     1501 gccaggccca tgtgagcgaa gttgacgcgg caacaggtat tgtacggttg gtttatgacc
     1561 aggctttttt gcagcatgcc cataccgatc gttatgtcag tcgcgggatc gccgatcgcg
     1621 catgccagca ggaaggctat acccacgcgg tcccctttgg tcagccagta ggcaactgta
     1681 gcctttttgc gggttcctta tgtttgaata cggaattcac tttgtcatat cagtgccatc
     1741 attccgcctt ccctgtcttt ctctaaatat tttaatcagc gaggggatct tcgctgatta
     1801 aagaaatagc ggtaatgcgt tttattccca ttcgtatttt taataattga agtttatatt
     1861 ttaccttttg caaataataa aataacaaat tatagtgacg ccacagcaac aaatataacc
     1921 tttgtggagc actatcatgc tgaaaccaga aatgattgaa aaacttaatg agcagatgaa
     1981 cctggaactg tactcttcac tgctttatca gcaaatgagc gcctggtgca gctatcatac
     2041 cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag gaagagatga cgcatatgca
     2101 gcgtctgttt gattacctga ctgataccgg caatttaccg cgtattaata ccgttgaatc
     2161 tccgtttgct gaatattcct cacttgatga attattccag gaaacctata aacacgaaca
     2221 attaatcacg cagaaaatta acgaactggc tcatgctgca atgaccaatc aggactaccc
     2281 aacatttaat ttcctgcaat ggtatgtttc tgagcagcat gaagaagaga aactgttcaa
     2341 atcgattatt gataaattaa gcctggcagg caaaagcggc gaaggtctgt attttatcga
     2401 caaagaactc tctaccctcg acacacaaaa ctaatgctct caggcggcag gttttctgcc
     2461 gccgtttcaa atcagtgacg gcaaatctta ctctgatcgg tcgaaaaacc ttcttctgaa
     2521 ggtataaatt tgccttttgc tgccagaaac gctaccagct ctcccgcagt catcccttct
     2581 gccgagcagg tgtgaaaacg tgcctgttca ccaaaacgcg ctttaattgc tgcttccaga
     2641 ctggcatgcg tatattgctc gcctgattca atcatcatat ttaacacttc atgaccgtga
     2701 atagagtcca tcgtccctcc tcaaaaaaag cctagcgtag cgattgccgc ttatgaagac
     2761 tttgcgccag cgcaggactg aatgcttttt attgtacatt tatatttaca ccatatgtaa
     2821 cgtcggtttg acgaagcagc cgttatgcct taacctgcgc cgcagatatc actcataaag
     2881 atcgtcagga cagaagaaag cgtgaaaaac agaaccctgg gaagtgtttt tatcgtggcg
     2941 ggaaccacaa ttggcgcagg catgctggca atgccgctgg ctgcggccgg tgttggtttt
     3001 agcgttacgt taatcttgtt gattgggctt tgggcgttga tgtgctacac ggcgctatta
     3061 ctgctggagg tgtaccagca tgttccggca gataccggtc ttggcacgct ggcaaaacgc
     3121 tatctgggac gctacggtca atggctgacg ggcttcagta tgatgttctt aatgtatgct
     3181 ctgactgcgg catacatcag cggtgccggt gaattgttgg cctccagcat cagcgactgg
     3241 acaggtattt caatgtcggc aaccgctggc gtgctgttgt tcacttttgt tgccggtggc
     3301 gtggtttgtg ttggaacatc gctggtcgat ttatttaacc gttttctgtt cagcgccaaa
     3361 attatttttc tagtggtgat gctggtattg ctgctaccgc atattcacaa agtgaatctt
     3421 ttaaccctgc cgttgcaaca ggggctggct ctgtcagcaa tcccggtgat ttttacctca
     3481 tttggttttc acggtagcgt gccgagtatt gtcagctata tggatggcaa cgttcgtaag
     3541 ctacgctggg tgtttataac cggtagtgcg atccccctgg tggcatatat tttctggcag
     3601 gtggcaacgc ttggcagcat tgattcaaca acctttatgg gattgctggc taatcatgct
     3661 ggattaaacg ggctgttaca ggcgttacgc gaaatggtgg cctctccgca tgttgagctg
     3721 gcagtgcatt tatttgctga tttagccctc gccacgtcat ttctcggcgt tgcgttaggc
     3781 ttatttgatt atctggctga tttatttcag cgttcaaata ccgttggtgg acggttgcaa
     3841 actggtgcaa ttacctttct gccgccgttg gcgtttgcac tgttttatcc acgaggattt
     3901 gtgatggcgc tgggttacgc cggtgtggcg ctggcggtac tggcattgat tatcccttcg
     3961 ctgttgacct ggcaaagcag aaagcacaat cctcaggcgg gttaccgggt gaaaggtggt
     4021 cgtccggcgc tggtggtggt gtttctctgt ggtattgctg tgattggcgt gcaatttttg
     4081 attgcggcag ggttgttacc agaagtgggg tgatcagata gcctcaaatt ccttattggg
     4141 tgccagaatt aacgctgaca cccaatttga cctcttaatg caggcagcac tgcttaaatt
     4201 tcttaccact accgcacggg caaggatcgt tacgccccgg tttctcttct gctttgatcg
     4261 gttgctgaac agctttttcc tgcggatgcg ccatccagta cgcatgtaga tcaagcgccg
     4321 ccagtcgaat ggcatctacg ctctcttcaa acgcttctgg cgacattttc tctacccgct
     4381 cgaagttttc ctcagtaccg tgcagcgcaa tcgcctccag cgctggtttt aacgaatcgg
     4441 gcaacgttga ccagtcagaa agtgccacgc cccgcatata gccaaagcac cactcctcaa
     4501 caatcgtcag ctcgctgcca tcaacttctc gcaagccgaa taacggctca aactgctccg
     4561 ggaattcgtt cagacgctct gcggtatcgg ccatatgttg aaaagccaga ttcataaagc
     4621 gcgtcatctc tttctctgac gcccagcgcg gcacatagtc agccccaccc cacacggcaa
     4681 ccagccactg ttccggttca atctcttgcg gagaactcaa caccgccgtc aataaaccgt
     4741 ccagctccgc cacatcaagg atggcgtggt cagtgttgta tttggtcaga atatcgtcca
     4801 gccattccaa ctcactttcg tttaacggtc ccgttttcat acgcttttcc ttgtggatct
     4861 caactcgcca gcacctatct tacatgccgg tccgtatcag agatactttt tgagtggctt
     4921 tgctggtgat taaaaattag ggagggtgta acgacaagtt gcaggcacaa aaaaaccacc
     4981 cgaaggtggt ttcacgacac tgcttattgc tttgatttta ttcttatctt tcccatggta
     5041 cccggagcgg gacttgaacc cgcacagcgc gaacgccgag ggattttaaa tcccttgtgt
     5101 ctaccgattc caccatccgg gctcgggaag aaagtggagg cgcgttccgg agtcgaaccg
     5161 gactagacgg atttgcaatc cgctacataa ccgctttgtt aacgcgccaa attcttcagg
     5221 cctttcagcc agacatccgc ttgacgccga tgtcttttaa actggagcgg gaaacgagac
     5281 tcgaactcgc gaccccgacc ttggcaaggt cgtgctctac caactgagct attcccgcat
     5341 tcatcaagca atcagttaat cacttgattt tattatcgtc tggcaatcag tgccgccgtt
     5401 cgatgcgttg cattctactt acctggcgcg atgagtcaac gatatttttc accacttttg
     5461 atcgtttgct gaaaattacg ccgaaacgat cactgatcaa gcaaatctgc acgcgcagcg
     5521 ctcaaatatt gcaacattga ccacagagtc agtaccgcag ccacaaagaa aagtgcaata
     5581 ccggcgtact caacccaaat gttcggacgc cacagcagcc aggccaacgc caccatctgg
     5641 gcagtggttt tcactttccc aatccaggag acagccacgc tactgcgttt acccaactcc
     5701 gccatccatt cgcgtagcgc agaaataata atttcacggg cgatcatcgt tgccgccggt
     5761 agggtcaccc accagctgtg ataatgctcg gttaccagca ccatggcgat agccacgaga
     5821 actttatctg ctacagggtc aaggaaagca ccaaaccggg tactctggtt ccagcggcgt
     5881 gccagaaaac catcgaacca gtcagtcacc gccgcgacgc agaaaatgag cgcggcggca
     5941 aacggcgacc aggtgacagg cagataaaag accaatacaa agaatgggat aaggatgaca
     6001 cggaacagtg taagcaacgt agggatatta aattgcataa tgacgggtaa ctatctgttg
     6061 tcagtaagat tacccctatg ttgctacaga gacatcaatg tttcaacgac cagaagatct
     6121 tttctgccag accttgcgaa atacccggca cttttgcaat ttcctcgacg ctggcgttac
     6181 gtaaaccttg caaaccgccc atatatttca acaacatttg ccgacgtttt ggcccgacgc
     6241 cttcaatggt ttccagggag ctggtatttt tgaccttcgc ccgtttttta cggtgcccgc
     6301 caatcgcgtg atcgtgtgat tcatcgcgaa tatgctggat aacatgcagc gcgggagaat
     6361 ctggcggcaa actgaatccc tcaccttccg gctcaaagaa cagtgtttcc agcccagcct
     6421 tacgatctac tcctttggca acgccaagta gcagtggatg atttttatcc catgagacat
     6481 ccagttcggc gaagacattt ttcgcctgcg caagctggcc tttgccgccg tcgataagga
     6541 tcacatccgg gatcttactg tcgtcaatgg ctttaccata acgccgacgc agcacctgat
     6601 tcatcgccgc ataatcatcg cccggcgtga tgcctgtaat gttatagcgc cgatactccg
     6661 cacgcagcgg gccgttagca tcaaacacca cacaggaagc gacggtttgt tcgcccatgg
     6721 tatggctgat gtcaaagcac tccatccgct tcacttccgg caatttcaac acgctggcaa
     6781 gcgcggtcag tcgctggtga acggtagatt gctgcgaaag tttgctggtt aaggccgtcg
     6841 ccgcattggt gcgcgcgagt ttcagataac gcgccctatc gccgcgaggt ttggtttgaa
     6901 cattaatctt gcgtcccgcc agttctgaaa gggaatcggc gagcagcgtt ttatcgctaa
     6961 gattaaaatc gagcaggatc tcacccggta aggtgcgcat ctggctgcct tgtaaataga
     7021 actggcctac gaaggtttct accacctcgc tcagttccgt accgccaggc actttcggga
     7081 aatagctgcg gctgccgagc actttgccct gacgaatgaa caatacgtgg acacaagcca
     7141 tgcccgcatc gaacgccaca ccaataacgt cgaggtcgtc gccggtattg gaaacgaatt
     7201 gtttttcggt gacgcgtcgc accgcctgaa tttggtcgcg aatacgtgca gcttcttcaa
     7261 actccagatt ctggctggca gtttccatac gactaatgag ttgcgtaagc acctgatcat
     7321 ctttgccaga caaaaacagg cgcacatact cgacctgctg agcgtattct tcttcactca
     7381 ccagtccttc aacgcacggt cccagacagc gccctatctg gtattgcaga cacggacgcg
     7441 agcgattgcg ataaacacta ttttcgcact ggcgaatggg gaaaatcttt tgcagtagcg
     7501 ccagtgtttc acgtacggca tagccattcg ggaacgggcc gaaatattca cctttggcat
     7561 gcttcgcacc acgatgcatc gccagacgcg ggtgggtatc gccactcagg aagataaaag
     7621 gataagattt atcatcgcgt agcaaaacgt tgtaacgcgg ctgatagagt ttgatgtagt
     7681 tgtgttccag caacagcgct tcggtttctg tatgagtaac cgttacatca atttgctgga
     7741 tctgggcgac cagcgcttcg gttttgcgcg aagcgaggtt gctacggaaa tagctggaaa
     7801 gccgtttttt caggtctttc gctttgccga catagataac cgtaccacca gcatcgtaca
     7861 tgcgataaac gcctggctgg ctggttacgg tttttaaaaa cgcttttgcg tcaaactgat
     7921 cactcactga cttgataatg tctccgcatt acacagacca tggcgaattg ccaggtgagt
     7981 cagctcaaca tcgccatgaa tgtttagttt actgaacata cgatagcggt agctgttcac
     8041 cgttttcgga ctgagattga gctgttctga gatctcattg accttctggc ccttggtgat
     8101 catcagcata atctgcaatt cacgttcaga caaactggca aatgggcttt ctgttttttc
     8161 tggttcgatc tggcttaacg ccatttgttg agcgatgtca gaagcaatgt aacgctgccc
     8221 tgaatagaca gaacgaatcg cactcacgac ttcctgcgga gccgcgcctt tgctgaggta
     8281 gcccgcagca ccggcctgca tgactttcgc tggtaaaggg ttttctgtat ggacggtaag
     8341 catgatgatt ttgacatcag ctgtggaacg cgcgatttta cgcgtcgcct caagaccgcc
     8401 aatgcccggc atactcatgt ccattagcac cacgtcaacg gcatttgccc gacaccactt
     8461 aacggcgtct tcaccgcacg atgcctcacc gacgactttt atacccttta tatcttccag
     8521 aatgcgtcgt atccctgcgc gcaccagttc gtggtcatca acaagtagaa cgttgatcaa
     8581 aggaatatct ccagaaatag ggataacgct actgatagtt agtcattcgt atattagcgg
     8641 tttttattgc aactttgaaa cgttaaaaat ggtgggtatt cgatttttct ctcgtttttg
     8701 tcaattcgat tgtccacagt tatggtcacg cccgtcacca acggcttacc ggatcgtttc
     8761 acagtaagcc ctgcgacgaa atgtgacaaa aattaccttt attcagcaaa aatgaaaatc
     8821 agcccgctga atatgtaaca ttaattaacc cgaacaatac gggcaaaaaa cattcatgat
     8881 aaaaatattt attggtcatt atattaacgc atttaatagc accgttgacg atacgctcaa
     8941 aaaacaacca ctgctatttt tagcaaagct tatggtatac tccgccgcct taacattatt
     9001 caccgcaaat tttcattgca acatgacgag gaaaataaat gagtacgcct gatttttcta
     9061 ctgccgagaa taatcaagaa ctggcaaatg aagtctcctg cctgaaagcg atgctgacgc
     9121 tgatgctgca ggcgatggga caagctgacg cgggccgcgt gatgttaaag atggaaaaac
     9181 agcttgcgct gatcgaagac gaaacccagg ctgcagtatt ttccaaaacg gttaagcaaa
     9241 ttaaacaagc ctaccgtcag taatataaaa ccggctgata gcgtgccttt cagccggttt
     9301 ttgcatctgg cacgcaggac agaaaagaga tcaaattaag ccagtagcgg ccgcgtaaca
     9361 ggcaacctgg gtcttattcg gtgcattgat ttttttctgc atgtttttct gatggaaatt
     9421 gaccgtattc tcagagattg acaaaatcat cgctatctct gctgatgtct tcccttccgc
     9481 cgtccacctc agaatttctt tttcgcgctt gctgaaattc atctctggcg tcatcactat
     9541 ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact gcatttttaa
     9601 ttgcagttca tcactaagaa tgggtatttc gcgcgcgctg caacgggaaa aggacaaaaa
     9661 gcccagcgcc cggtttggca gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc
     9721 gcgcgcggct tcccataacg gctgtgcttc gttgaataag tcatcattcc acattagatg
     9781 gccctgacta aagttttcag ggttcagcac cggatcaatt gcgagaaagt tttttgcctg
     9841 ataataacta acccacgcct cagggtaatt ggtgtagaaa gccactttag gtcgcgtgaa
     9901 tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct gagcctgaag
     9961 ctctatttca tggtagacct cttctgcggc ctccatcctc tgaaaacgca acagcatcgt
    10021 gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc ctgagagtga
    10081 gtatcattta taatgataat tcaaactatc acataaataa gatttatata taatttatat
    10141 tattcaggca atgaattact tttgcaagcc atcacattct cttatgttat taatgagtta
    10201 tgctgatttg ttaagcagtt ttatcacgct tgaaatagcg tcccgccccg acaggtgaat
    10261 cgtcggggct gattttttct tattattgca gcagaaactt ctcgaggaac tggcgggtgc
    10321 gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact atccgcccct
    10381 ggtccataaa aatcgcccgg tccgcaacat cccgggcaaa gctcatttcg tgcgtcacaa
    10441 tcaccatcgt gcgcttttcc tgcgccagct gacggatggt gttcaggact tcgcccacca
    10501 gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga cgcattgcca
    10561 gcgcacgtgc aatcgcaaca cgttgctgtt gaccgccaga caaacgacgt ggatagctgg
    10621 tttctttacc ggccagccca actttcgcca acagctcgcg agcgcgcgcc gtggcctctt
    10681 ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc accgtacgat
    10741 gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac tggcgaatca
    10801 gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca acggtgatcg
    10861 ttcccgcttc cggttgttcc agcagattta tgctgcgtag caacgtggtt ttgccggaac
    10921 cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg ataccgtgca
    10981 gcaccgtctg accgtgaaat tttttcacca agttcttaac ttcaatggca ctcattttgg
    11041 ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg ataatactgt
    11101 cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg
    11161 cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg cggccagcga
    11221 cgtatccttt accaggctga taaagctgtt cgacagcggc ggcaacgcca cccgcgccgc
    11281 ctgtggcaaa atcgcgcgac gcatagtctg ccatggcgtc ataccaatac tggcagctgc
    11341 ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agtgtttcgg cggcataggc
    11401 ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt cgataccaaa
    11461 ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggtgtac cacgaaaaat
    11521 agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc gcatcagcgc
    11581 gagaataaac ccgagcagta agccaaaaaa catgccgccg atactgagtt gcagcgtata
    11641 cccagcccct ttcagcagga acggcaagga atcaataacc agttgtatac tttcttgcat
    11701 gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc accgccggta
    11761 tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc gtcaatcagc
    11821 cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag ccgcgccagc
    11881 aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca
    11941 tcccagagta taatttccgc tgatgcggtc agctccagtt ctttcgcaat cgcctgttgt
    12001 aggttaacca ctttcggcaa ctgatcggca acggaacgcg acacggtcac gccaatcagt
    12061 tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt tccggcactg
    12121 cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg cgcgatttcc
    12181 agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac cggaatgaca
    12241 tacgggcgaa agccttgtgc ttcgactcgc gtcgccagct cttccagttg ggcattggga
    12301 tcggtcagtg cttcgcacat ttcaatctgg gtattgaaca gatccagcaa caagcgattg
    12361 ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag cgccacacag
    12421 tgcagaccga gtttcgccgc aactgcggca gtctgccgca catggttaga ctggatcgcc
    12481 ccggcagtaa tcagcgtatc ggcaccttcg cgcagagcat ctgctgcgag aaattccagc
    12541 ttacgtaatt tattgccgcc cattgccatg ggggtgacgt catcccgttt gatgaaaatt
    12601 tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggtgttgg cgcgccgata
    12661 aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc cgatgtgtgt
    12721 tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc gctggtgaaa
    12781 gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac catttttcgg
    12841 aaagggcttg cagagtgcca tctttttgca tttccgcaat tgcatcgttc actgctttca
    12901 gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag aatgcttcac
    12961 cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc agacggtcaa
    13021 cgaggatcgc atcgatacgg cctacgcgca gatcctgata tttggtcggg tcatcatcat
    13081 aggtacgcac atcgacgccc tgaacattct gccgcagcca ctcttcatag ttggtgccca
    13141 gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg ccttcgttac
    13201 cttttttcac cagcgcctga ataccagaaa tggtgtacgg ggtagagaaa tcgtattttt
    13261 tcttgcgctc atcagaaatg gtgacctgat taatcaccac atcaatgcgt ttagagtcca
    13321 gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg ccaagatgtt
    13381 ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca tccccctgaa
    13441 aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgcgc tctttaactt
    13501 tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc agcgctacgg
    13561 ccatcacact catcaatgcc tgacgtccca gatgtgctaa tttcatattt accccgaatg
    13621 ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca actccgctac
    13681 atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc tgcacaagtc
    13741 cggtacacgt ttgacgctga tagtgttgat acttccgtaa tgcgatgcgg taattgttgg
    13801 tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc tgcagcggat
    13861 cgagggaaat attttgctcg tgtagatgat tccccaaacg gcgcaaacga acgacatatt
    13921 cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaattgctta aagccgataa
    13981 tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg
    14041 cccatgattt ttgttccgtt tgccagccat tttcatcaag cagcgcgtcc aggcgggaaa
    14101 tctcgatttt attcactatt ttgccgtcgc gaacgagggt aatgcgatcg agtaatttac
    14161 ggcaatgcgc gcaatgagtc tggctgtgtt taaagtcttt aagatagcgg cttaatggcc
    14221 gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta aagtgcggca
    14281 tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatagcc tggctgtgta
    14341 actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg agattcagct
    14401 cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt tcgatggctt
    14461 ccatcacccg ctgacgcaga ttactgtcca gcagttgttg tagcgggttt tctcgctgat
    14521 gatcatcagt aaccagttcg atgctatcgc cgtgctcttc gcgccactca tcgtaggaga
    14581 agagctggct gttattggtg tcgagcaaca tttggcgata atcggcaata tcgatcccta
    14641 aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc agttgcccta
    14701 ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag tcacggctgc
    14761 gaagttcatc cagcatagcg ccacggatac gctgcactgc gtaagttgta aatgccgttc
    14821 cttgtagggc gtcatagcgt tcgacggcat taagtaaccc aatgccgccc gcctgtagca
    14881 gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct tcgtgacgca
    14941 ccagcgggac ataacgctgc catagcgagt gtttatccat tacaccttca gcggtataga
    15001 gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta tccgtttctg
    15061 cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg gttaccggta
    15121 aattgcgggc agaaaaaacc ccgccggtgg cgggggttga gcgataagtg taaattaacg
    15181 cagcagagac agtacgttct gcgtggtctg gttagcctgc gccagaacag aggtacccgc
    15241 ttgttgcagg atctgcgcac gagacatgtt agacacttcg gtcgcgtagt cagcatcttc
    15301 gatacggcta cgggcagaag acaggttgtt tacggtgttg ccaaggttgg tgatagcaga
    15361 gtcgaaacgg ttttgtactg caccgaggtc agaacgcaga ttgtcaactt tagccaatgc
    15421 tttgtcgata gtttcgagcg ggttggtggt agattgcaac gattttgctg catcttcgtt
    15481 taccagaatc gggctaccac cttctgattt gctcagatac atgactttat tgttaccaga
    15541 agcagtctcc gttatcgttt taccatctgc actaacatcg taagttgcac cgttaacaac
    15601 taacgtgctt cctgttttct tagctgcatt gagatcaaga tcggaaagtg tggcagcctt
    15661 gttatcaact ttggttgtcg tataaaggcc agcgctattt ttgtaaactg tctcactcgt
    15721 aatcaaagaa gcttttttgt cagcatctgt cacagtaaat gtcactgact taccatcaat
    15781 ttctgcagaa tatacaccat tcccattgcc atccgctgca gttgttgttg tgaattcaac
    15841 tccattaaac gtgaatttat cattctgtgc tttattctta gccgctgctg cgagtgctgt
    15901 agcatcaatc cctgtaccag ctatgtttgt gtcagattta gttgtcagtg aaccatctgc
    15961 tgcactaaca aaaatatcat tccctgtagt atctttaaca gctccgctat ctacgttaac
    16021 agtataagca tcaccgccaa catcatagtt atcagtacct gtcgctttaa atttagaaat
    16081 caggtcactg ccagttgctt tctgcgcgcc atcgatatta aaaccgtcca ggccgagagt
    16141 tttcgcatca atttttgcca gattgatagt gatggtttca ccatcattag caccaacctg
    16201 aattttcatt tcattatttt cagcaaggac tttcacgccg ttaaactgag tttgctcaga
    16261 tacacggtca atttcttcca gacgttgagt aatttcagcc tggatagaag aaagatcgct
    16321 gtcagagtta gtaccgttag ttgcctgaac agtcagttca cgtacacgct gcaggttgtt
    16381 gttaatttca ttcagcgcac cttcagtggt ctgcgcaaca gaaataccat cattcgcgtt
    16441 acgggaagcc tgagtcagac ctttaatatt tgctgtaaaa cggttagcaa tcgcctgacc
    16501 tgctgcgtca tctttagcac tgttaatacg caggccagaa gagagacgtt caatggcgga
    16561 gctcagagaa gactgagatt tgttcaggtt attctgggtc aacagcgaca ggctgtttgt
    16621 attaatgact tgtgccatga tcttttcctt atcaattaca acttgatgtt attgggctgt
    16681 tgcccacggt ttctcaccgt accccttgta tcggcacctg aatttcgaac tttagaaaat
    16741 tttagtttct ctcctgtttt ttcttaacgg ctgaaatatc cgtaatatac aactctactc
    16801 tgcgcatcat cttcgtgaat caactattaa actttgcgca caaattgccg ataacccgct
    16861 tatctactgt ttgcaatcaa aaggaattag gtatggcttc atttacatct cttggcgttg
    16921 gttcgaatct gccgctggat acattgctaa acaacctgac catcgctgaa aaaaaacgct
    16981 taaacccaat tactcagcaa cagagtgaca ataccgcacg tctgacagcc tatggcacat
    17041 taaaaagtgc attagaaaaa ttccagactg caaatactgc gttaaataaa gccgatctgt
    17101 tcaaaagcac taccgttacc agtagcaatg aagaccttaa agtttccacc gaagcaggtg
    17161 ctgcccccgg tatctatacc atcagcgtaa cccaactggc gcaggcgcaa tctttacgaa
    17221 ctgattcccc gacaatcatt gcatcaacaa aagatgcact gggtgatgag tcatcagata
    17281 cacgcacaat aaaaattacc caagacggtc gtaaagagcc gctggaaatc aagctcaata
    17341 aagatcagac gtcattggat gagatcagca aagccattaa cgatgccgat agtggaattt
    17401 ccgctagtat cgttaaagtc aaagatggta actatcaatt ggtattgacc gccagtgaag
    17461 gtctggccaa taaaatgacc atatctgttg agggtgacag taaattaaat gatctgctgg
    17521 cttatgacag caagaccaac accgggaata tgaaggaact ggttaacgcg caaaatgcac
    17581 agctaaacgt taacggtatc gatattgaac gttcaagtaa caaaattacc gatgctcctc
    17641 agggagtgac tctcgatttg accaaaaaag tcactgacgt ccgggtcacg gtgacaaaaa
    17701 gcaacgataa agcaaccgaa gcaattaaag gctgggtgga ttcatataac tccctcattg
    17761 acacctttaa caccctgaca aaatataaag aagtagaccc aggtgctgaa gcacaagata
    17821 aaaataatgg tgcacttctc ggagacagcg ttgtacgtac aattcaaagc ggcatccgcg
    17881 cacaatttgc aaacggtgcc agcgacagtg ccttcaaaac attaaacgag atcgggatta
    17941 agcaggacgg cacgacgggt aagctgaaaa ttgatgatga taaactcaaa aaagtcctga
    18001 atgaaaatac agcatccgta cgtgaattgc tggtaggcga tggtaaagaa acaggaatta
    18061 ccaccaaaat tgccaccgag gtaaaaggtt accttgccga tgatggcatt attgatagcg
    18121 cacaggacag tattaacgcc acactgaaaa aactgaccaa acagtatcta tccgtcagtg
    18181 ccagcattga tgacaccgtg gcgcgttaca ctgcacagtt tacgcaatta gataccatga
    18241 tgagcaaact gaataacacc agtacctatc tcagccaaca atttactgca atgagtaatt
    18301 cctgatcacg gaggtcagca tgtataccgc gagcggctca aaagcctatg cacaaattgg
    18361 cgtcgaaagc gccgtaatga gcgccagcca gcagcagctg gtcaccatgc tatttgatgg
    18421 agtgctgagc gcactggtta gagcacgcct gtttatgcag gacaacaatc agcaaggcaa
    18481 aggcgtctct ttgtcaaaag cgatcaacat cattgagaac ggactgcggg tgagtcttga
    18541 tgaagagagc aaagacgaat taacccaaaa cttgattgct ctttatagct atatggtcag
    18601 gcgcttgctg caagccaatt tacgcaacga tgtctccgca gtcgaagaag tggaagcatt
    18661 aatgcgcaat attgccgatg cctggaaaga gtcgttactc tccccttctt tgattcagga
    18721 cccagtctga tgaaccacgc accgcatttg tatttcgcct ggcaacaact cgtcgaaaaa
    18781 agccagctca tgttacgcct ggcaacagaa gaacaatggg acgaactcat cgccagcgag
    18841 atggcgtatg tgaatgcggt gcaggagatt gcacatctga ctgaagagat tgacccgtcc
    18901 accacgatgc aggagcagct ccgcccgatg ctgcgcctga ttctcgacaa cgaaagcaag
    18961 gtaaagcagt tattacagat tcggatggat gaactggcga aactggtcgg tcagtcatcg
    19021 gtgcaaaaat cggtgttaag tgcctatggt gatcagggcg gctttgtgct ggctccgcag
    19081 gataacctct tttgaatctg aacgagtcga tggctcgcga ataatccgat tacggctacg
    19141 cttctaatgt tccccttgaa tggagtcgaa gaatgcgtaa tcccacgctg ttacaatgtt
    19201 ttcactggta ttacccggaa ggcggtaagc tctggcctga actggccgag cgcgccgacg
    19261 gttttaatga tattggtatc aatatggtct ggttgccgcc cgcctataaa ggcgcatcgg
    19321 gcgggtattc ggtcggctac gactcctatg atttatttga tttaggcgag tttgatcaga
    19381 aaggcagcat ccctactaaa tatggcgata aagcacaact gctggccgcc attgacgccc
    19441 tgaaacgtaa tgacattgcg gtgctgttgg acgtggtagt caaccacaaa atgggcgcgg
    19501 atgaaaaaga agctattcgc gtgcagcgtg taaatgctga tgaccgtacg caaattgacg
    19561 aagaaatcat tgagtgtgaa ggctggacgc gttacacctt ccccgcccgt gccgggcaat
    19621 actcgcagtt tatctgggat ttcaaatgtt ttagcggtat cgaccatatc gaaaaccctg
    19681 atgaagatgg catatttaaa attgttaacg actacaccgg cgaaggctgg aacgatcagg
    19741 ttgatgatga attaggtaat ttcgattatc tgatgggcga gaatatcgat tttcgcaatc
    19801 atgccgtgac ggaagagatt aaatactggg cgcgctgggt gatggaacaa acgcaatgcg
    19861 acggttttcg tcttgatgcg gtcaaacata ttccggcgtg gttttataaa gagtggatcg
    19921 aacacgtaca ggaagttgcg ccaaagccgc tgtttattgt ggcagagtac tggtcgcatg
    19981 aagtcgataa actgcaaacg tatattgatc aggtggaagg caaaaccatg ctgttcgatg
    20041 cgccgctgca gatgaaattc catgaagcat cgcgcatggg gcgcgactac gacatgacgc
    20101 agattttcac gggtacatta gtggaagccg atcctttcca cgccgtgacg ctcgttgcca
    20161 atcacgacac ccaaccattg caagccctcg aagcaccggt cgaaccgtgg tttaaaccgc
    20221 tggcgtatgc tctaattttg ttgcgggaaa atggcgttcc ttcggtgttc tatccggacc
    20281 tctacggtgc gcattacgaa gatgtcggtg gtgacgggca aacctatccg atagatatgc
    20341 caataatcga acagcttgat gagttaattc tcgcccgtca gcgtttcgcc cacggtgtac
    20401 agacgttatt tttcgaccat ccgaactgca ttgcctttag ccgcagtggc actgacgaat
    20461 atcccggctg cgtggtggtc atgtcgaacg gggatgatgg cgaaaaaacc attcatctgg
    20521 gagagaatta cggcaataaa acctggcgtg attttctggg gaatcggcaa gagagcgtag
    20581 tgaccgacga aaacggcgaa gcgaccttct tttgcaacgg cggcagcgtc agcgtgtggg
    20641 ttatcgaaga ggtgatttaa attcatcccc ggcggcaagc cggggagatt tcattacggc
    20701 agtggcgtcg gtaacggcgc tttatccagc gcggcggcac actctgccgt cgggcgatcc
    20761 acacgctcca tcgtcatacc atcgtactca atggtgttac cgtcacgctc aacctcgtac
    20821 agctcacgtt ttacggtaac gttagtgaga tcatcagaca tcaatgtcaa cttgcccggc
    20881 acggcaatca cccgctgcca ctgacggcaa tccagggtat cgccctcttt agtgacaatc
    20941 aggctgccta tcgcttccgg actgaccagc gcacgttgcg gccctttggt ctgccagtag
    21001 cccgctagcc agtccggcgc gggggttttc acgacattgt tatagttttc gacttcggca
    21061 cagcccgcca gcaacatgag cgcgcttgca attgcgagtt ttttcatcat tcgtcctgca
    21121 tgagaagaaa agaggattgt ggcattaaag ccctgatgtc gccagccttt ccaggttcag
    21181 agagaaattt gatctaacta ttatttttgt gaattattac cccgaaaata aatttgcatg
    21241 atgtatgatt cgcgattctt gtttcaaacc ttctgagttc agaggctaca ttcatgtcat
    21301 ggcagcaatt caaacacgcc tggttgatta aattctgggc acccatccct gcggtcatcg
    21361 cggcgggtat tctctccact tactattttg gcattactgg caccttttgg gctgtcacgg
    21421 gtgaatttac ccgttggggc ggccagctcc tgcagctgtt tggcgtccat gctgaagagt
    21481 ggggttattt taaaattatc catctggaag gatcgccatt aacccgcatc gacgggatga
    21541 tgatcctcgg catgtttggc ggctgctttg ccgcagcgct gtgggccaac aatgtcaaac
    21601 tgcgcatgcc acgcagccgt atccgcatta tgcaggccat tattggcggt attatcgctg
    21661 gttttggcgc gcgtctggca atgggctgta acctggcggc gttctttacc ggtattcctc
    21721 agttctcgct gcatgcctgg ttctttgcca tcgccactgc cattggttca tggtttggcg
    21781 cacgctttac cctgctgccc atcttccgta ttcccgtgaa aatgcagaaa gtttctgccg
    21841 cctcaccgct gacgcaaaaa ccggatcagg cgcggcgtcg ttttcgtctc gggatgctgg
    21901 tctttttcgg tatgctgggc tgggcgctgc tcacggcgat gaaccaacca aaactggggc
    21961 tggcaatgct gtttggcgtc ggctttggtt tactgattga acgtgcgcaa atctgcttta
    22021 cttcggcgtt ccgcgatatg tggatcaccg gacgtaccca tatggcgaaa gcaatcatta
    22081 tcggtatggc ggtgagtgcc atcgggatct tcagttacgt acagttaggc gttgaaccca
    22141 aaatcatgtg ggcgggacca aacgcggtaa ttggtggttt actgtttggt tttggcatcg
    22201 tgctggcagg cggctgcgaa accggctgga tgtaccgcgc ggtagaaggc caggtgcact
    22261 actggtgggt cggtctgggc aatgtgatcg gctcgacgat tctggcgtat tactgggatg
    22321 atttcgctcc ggcgctggcc accgactggg acaaaatcaa cctgctgaaa acctttggcc
    22381 cgatgggtgg cctgctggtg acatatttgc tgttgtttac tgcgctgatg ttgattatcg
    22441 gctgggaaaa acgcttcttc cgccgtgcgg caccgcagac tgctaaggag atcgcatgaa
    22501 aaatatcgtt cctgattacc gccttgatat ggtgggtgaa ccctgccctt atccggcagt
    22561 cgcaactctt gaggcgatgc cgcagttaaa aaaaggggaa atcctggaag tggtgagcga
    22621 ctgtccacag tcgatcaata atattccact ggatgcgcgt aatcacggct atacggtgct
    22681 ggatattcag caagacgggc cgaccattcg ttatttaatt cagaagtaat ccctcattcc
    22741 cgctgtggcc cacagcggga acatcgttgc ctctgacaca tagattttac ccgccccctc
    22801 tcctattctt ttcagtatga tctcaggagg tttctatgtg tggacgcttt gcccaatccc
    22861 aaacgcgtga agattacctt gcgcttctcg cggaagatat tgaacgcgat attccctacg
    22921 atcccgaacc cattggcaga tacaacgtcg cgccgggaac caaagttctt ctgctcagtg
    22981 aacgtgatga acaccttcat ctggatccgg ttttctgggg atatgctccc ggatggtggg
    23041 ataaaccgcc gctgattaac gcccgcgtag aaactgcggc caccagtcgt atgtttaaac
    23101 cgctctggca acatggtcgg gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag
    23161 aaggcgacaa aaaacagcct tattttatct atcgcgctga tggacaacct gtttttatgg
    23221 ccgcgatagg tagcacaccg tttgagcgcg gtgacgaagc cgaaggattt ttaattgtca
    23281 ctgcagcagc agaccagggt ctggtagata ttcatgaccg ccgcccgctg gttctgtcgc
    23341 cagaagctgc acaggaatgg atgcggcaag agattggcgg taaagaagca tcagaaattg
    23401 ctaccaacgg ctgtgtgcca gcaaaccagt tcacctggca cccggtatcg cgcgcggtgg
    23461 gtaatatcaa aaaccaggga gcggagttaa ttcaaccggt ttgataagcc aggagactat
    23521 tgatcaataa tactgcgtca taagaaatct ctattagaca atagtagacg ctccttccac
    23581 atgcgccaca agaaacctct attccagtga cacaattacg cctgattatt tccctagccc
    23641 ggaatatgaa taaaaacatc aacaaagatt aaattattca aacgttactg ttgcgtctgc
    23701 ggtcgtagca acatccgacg tggtgatgag tgttaaaaga gacactctga tgttcagagg
    23761 gattatcgcg tttacggatc gtggatggca gaaaactttc aggatcaggt atccatttta
    23821 aaccagaaat tgagtgagtt tgccccatcc atgccccaag cggtgagatt ggaagtaatt
    23881 aacaacaggt tacaaaacct acacctgcat ggacatcact tcctgatacg ccgccaccag
    23941 cttattacgc acctgaatcc ccatttgcat agaaactgag gctttttgca tatcggtcat
    24001 cacatcgttt aacgccacgc cgggttcacc gagggtgaat ttttctgcct gcgtgcgggc
    24061 agctgtttgt gtatcgctaa tgcgatcgag cgtggcgtgc agctgcccgg caaaactaat
    24121 ggtcggttgc ggcagtgaat cctgcgcacg cgcactcatc gccgtagcct gtaactggct
    24181 gataacccct tcaatcccct gtatcgctga cattttcgtc tcctggataa tttctggtag
    24241 caaagcctac cagtaagtca ataagataaa ggcgctaaat aacaacaaaa aaacgggttt
    24301 attggcggat agaaaaaaac gaaagcacaa ataatggcag cgtcaatttt tcgagtttgc
    24361 tgacccggga gtgagtcttg ttccactttg ccaataacgc cgtccataat cagccacgag
    24421 gtgcgcgatg aatgcgactg cagcccagac aaaatctctt gagtggctta atcgcctgcg
    24481 tgcgaatccg aaaattccat tgattgttgc cggttccgcg gcagtggcgg tcatggtcgc
    24541 actgatcctg tgggcgaaag cccccgacta ccgcacatta ttcagcaatc tttccgatca
    24601 ggatggtggc gcaattgtca gccaactgac gcaaatgaat attccttacc gcttcagcga
    24661 agccagcggc gctattgaag ttccggcaga taaagttcac gaactgcgtc tgcgcctggc
    24721 acaacaaggt ttgcccaaag gcggcgcggt cggtttcgaa ctactcgatc aggaaaagtt
    24781 tggtatcagc cagttcagcg aacaggtgaa ttatcagcgg gcgctggaag gcgagctttc
    24841 tcgtaccatc gaaactatcg gcccggtaaa aggggcgcgc gtacatctgg caatgccgaa
    24901 accgtcttta ttcgtccgtg aacaaaaatc cccttctgca tcggtgacgg taaatctgtt
    24961 acccggccgc gcactcgatg aagggcaaat tagcgccatt gtgcatctgg tttccagcgc
    25021 cgttgctggt ctgccgccgg gaaacgtcac gctggtggat cagggcggac atctgttaac
    25081 ccagtccaat accagcgggc gcgatcttaa tgatgctcag ttgaaatatg ccagcgatgt
    25141 cgaaggccgt attcagcggc gtattgaagc aatcctgtcg cctattgttg gtaacggtaa
    25201 tattcacgcc caggtcacgg cgcagctgga cttcgccagt aaagaacaaa cggaagaaca
    25261 gtatcgccct aacggtgatg aatctcatgc ggcgcttcgt tcacgccagc ttaatgagag
    25321 cgagcaaagc ggttccggtt atccgggcgg cgtaccgggg gcgttgtcga atcaaccggc
    25381 acctgcgaat aacgcgccaa tcagcacgcc tccggcaaat caaaataacc gccagcagca
    25441 ggcgagcacc accagcaata gcgggccgcg tagcacacag cggaatgaaa ccagtaacta
    25501 cgaagtcgat cgcaccattc gtcataccaa aatgaacgtg ggcgatgtgc aacgtctgtc
    25561 agtcgcggta gtggtgaatt acaaaacctt gccagacggt aaaccattgc ctctcagcaa
    25621 cgaacagatg aagcaaattg aagatctgac ccgcgaggcg atgggctttt ctgaaaaacg
    25681 cggtgactcg ctcaatgtcg ttaactcgcc gttcaatagc agtgacgaaa gcggcggaga
    25741 actgccgttc tggcaacagc aagcgtttat cgatcagttg ctggctgccg gtcgctggtt
    25801 gctggtactg ctggtggcgt ggctgctgtg gcggaaagcg gtacgcccgc agttaacacg
    25861 tcgcgccgag gcgatgaaag ctgtacagca acaggcgcag gcccgtgagg aagtggaaga
    25921 tgcggtggaa gtccgcctga gcaaagacga acaactccaa caacgacgcg ctaaccaacg
    25981 tctgggggca gaagtcatga gccagcgtat ccgtgaaatg tctgataacg atccgcgcgt
    26041 ggtggcgctg gtcattcgcc agtggataaa taacgatcat gagtaacctg acaggcaccg
    26101 ataaaagcgt catcctgctg atgaccattg gcgaagaccg ggcggcagag gtgttcaagc
    26161 acctctccca gcgcgaagtg caaaccctga gcgctgcaat ggcgaacgtc acgcagatct
    26221 ccaacaagca gctaaccgat gtgctggcgg agtttgagca agaagctgaa cagtttgccg
    26281 cactgaatat caacgccaac gattatctgc gttcggtatt ggtcaaagct ctgggtgagg
    26341 aacgtgccgc cagcctgctg gaagatattc tcgaaactcg cgataccgcc agcggtattg
    26401 aaacgctcaa ctttatggag ccgcagagtg ccgccgatct gattcgcgat gagcatccgc
    26461 aaattatcgc caccattctg gtgcatctga agcgcgccca ggccgccgat attctggcgt
    26521 tgttcgatga acgtctgcgc cacgacgtga tgttgcgtat cgccaccttt ggcggcgtgc
    26581 agccagccgc gctggcggaa ctgaccgaag tactgaatgg cttgctcgac ggtcagaatc
    26641 tcaagcgcag caaaatgggc ggcgtgagaa cggcagccga aattatcaac ctgatgaaaa
    26701 ctcagcagga agaagccgtt attaccgccg tgcgcgaatt cgacggcgag ctggcgcaaa
    26761 aaattatcga cgagatgttc ctgttcgaaa atctggtgga tgtcgacgat cgcagcattc
    26821 agcgtctgtt gcaggaagtg gattccgaat cgctgttgat cgcactgaaa ggggccgagc
    26881 agccactgcg cgagaaattc ctgcgcaata tgtcgcagcg tgccgccgat attctgcgcg
    26941 acgatctcgc caaccgtggt ccggtgcgtc tgtcgcaggt ggaaaacgaa cagaaagcga
    27001 ttctgctgat tgtgcgccgc cttgccgaaa ctggcgagat ggtaattggc agcggcgagg
    27061 atacctatgt ctgataatct gccgtggaaa acctggacgc cggacgatct cgcgccacca
    27121 caggcagagt ttgtgcccat ggtcgagccg gaagaaacca tcattgaaga ggccgaaccc
    27181 agccttgagc agcaactggc gcaactgcaa atgcaggccc atgagcaagg ttatcaggcg
    27241 ggtattgccg aaggtcgcca gcaaggtcat aagcagggct atcaggaagg actggcccag
    27301 gggctggagc aaggtctggc agaggcgaag tctcaacaag cgccaattca tgcccggatg
    27361 cagcaactgg tcagcgaatt tcaaactacc cttgatgcac ttgatagtgt gattgcttcg
    27421 cgcctgatgc agatggcgct ggaggcggca cgtcaggtca tcggtcagat gccaacagtg
    27481 gataactcgg cactgatcaa acagatccaa cagttgttgc agcaagaacc gttattcagc
    27541 ggcaaaccgc aactacgcgt gcaccccgat gatctgcaac gtgtggatga tatgctcggc
    27601 gcaaccttaa gtttgcatgg ctggcgcttg cggggcgatc ccacactcca tcctggcggc
    27661 tgtaaagtct ccgccgatga aggcgatctc gacgccagtg tcgccactcg ctggcaagaa
    27721 ctctgccgtc tggcagcacc aggagtggtg taatgaccac gcgcctgact cgctggctaa
    27781 ccacgctgga taactttgaa gccaaaatgg cgcagttgcc tgcggtacgt cgctacgggc
    27841 gattaactcg cgctaccggg ctggtgctgg aagccaccgg attacaattg ccgctcggcg
    27901 caacctgtgt cattgagcgc cagaacggca gcgaaacgca cgaagtagaa agcgaagtcg
    27961 ttggctttaa cggtcaacgg ctgtttttaa tgccgctgga ggaagtcgaa ggtgtcctgc
    28021 ccggcgcgcg tgtttatgcc aaaaacattt cggcagaagg gctgcaaagc ggcaagcagt
    28081 tgccgctcgg tccggcgtta ttaggtcgtg ttctggacgg cagcggtaaa ccgctcgatg
    28141 gcctcccctc ccccgatacg acggaaaccg gtgcgctgat taccccacca tttaacccgt
    28201 tgcaacgtac accgattgaa catgtgctgg acaccggcgt gcgcccaatc aatgccctgc
    28261 ttaccgttgg gcgtgggcag cgtatggggc tgtttgccgg gtccggcgtt ggtaaaagtg
    28321 tgctgctggg gatgatggca cgttacaccc gcgccgatgt cattgtcgtg ggtttgattg
    28381 gtgaacgcgg acgcgaagta aaagatttta ttgagaatat cctcggtgcc gaagggcgtg
    28441 cacgctccgt ggtgattgcc gctccggcgg atgtctctcc gcttctgcga atgcaaggtg
    28501 ccgcctatgc cacgcgaatt gccgaagatt ttcgcgatcg tggtcagcat gtgttgctga
    28561 ttatggactc cctcacccgc tacgcgatgg cccagcgtga aattgcgctg gcgattggcg
    28621 aaccacctgc cactaaaggt tatccaccgt cggtgtttgc caaattaccg gcactggtcg
    28681 agcgtgccgg aaatggaatt agcggcggcg gctcgattac cgcgttttat accgtgctca
    28741 ccgaaggcga tgaccagcag gaccccattg ccgactctgc gcgggcaatc ctcgacggtc
    28801 acattgtgct gtctcgccga ctggcggaag ccgggcacta tccggctatc gatattgaag
    28861 cgtcgatcag ccgcgcaatg acggcgttga tcagtgagca acattacgcg cgagtgcgca
    28921 ccttcaaaca gctgttgtcg agttttcagc gtaaccgcga tctggttagc gtcggcgcgt
    28981 atgccaaagg cagcgatccg atgctcgaca aagccatcgc cctgtggccg cagctggagg
    29041 gctatttgca acaaggcatt tttgaacgcg cggactggga agcgtctctc caggggctgg
    29101 agcgtatttt cccgacagtg tcataaccca ggagataacg gcagatggca gaacatggcg
    29161 cgctggcgac cctgaaagat ctggcagaaa aagaggtaga ggatgccgcg cgcctgctgg
    29221 gtgaaatgcg tcgcgggtgt cagcaggcgg aagaacagct caaaatgctg atcgattatc
    29281 agaatgaata tcgcaataac ctcaacagcg atatgagtgc cgggatgacc agcaaccgct
    29341 ggatcaacta tcagcagttt atccagacgc tggaaaaagc cattactcag catcgccagc
    29401 aacttaatca gtggacgcag aaagttgaca ttgccctgaa cagctggcga gaaaagaaac
    29461 aacgtttgca ggcctggcag acactgcagg aacggcaatc cacggcggca ctgcttgcag
    29521 aaaaccgcct cgatcagaaa aagatggatg agttcgccca gcgcgccgcc atgaggaaac
    29581 ctgaatgatt cgcttagcgc ccttaattac cgccgacgtt gataacgcca cattgcctgg
    29641 cggcaaagcc agcgatgctg cacaagattt tctcgcgttg ttgagcgaag cattagcagg
    29701 cgagacaact accgacaaag cggcccccca gttgctggtg gcaacagata agcccacgac
    29761 aaaaggcgag ccgctggtca gcgagattct tgccgatgca caacaagcgg atttactgat
    29821 ccctgtggat gaaacaccgc ctgtcatcaa cgacgaacaa tccacatcaa caccattaac
    29881 caccgctcaa acgatgacgt tggctgcggt ggctgacaac aatacggcaa aagacgaaaa
    29941 agcggatgat ctgaatgaag acgtcaccgc aagcctgagc gccctttttg cgatgttgcc
    30001 gggttttgac aatacgccca aagtgactga tgtgccgtca accgtgttac cggcagagaa
    30061 accaacgctc ttcacaaaac tgacttctgc gcaactcaca acagcacagc ctgatgatgc
    30121 ccccggcacg ccagctcagc cattaacacc gctggtagca gaagcccaga gtaaagcgga
    30181 agtcatcagc acaccttcac cggtgaccgc tgccgccagc ccgctaatca ctccacacca
    30241 gacacagcca ctgcccaccg tcgccgtgcc tgtgttgagt gcaccgctgg gttctcacga
    30301 atggcaacaa tcattaagcc agcatatttc gctgttcacc cgccagaggc aacaaagtgc
    30361 agagttgcgt ctgcacccgc aggatttagg tgaagtgcaa atctccctca aagtggatga
    30421 taaccaggca caaatccaga tggtttcacc gcatcagcat gtacgcgccg cgctggaagc
    30481 agcgcttccg gtactgcgta cgcagctggc cgaaagtggc attcagttag ggcaaagcaa
    30541 catcagtggc gaaagcttta gtggtcagca gcaggccgct tcccaacaac agcaaagcca
    30601 acgcacagta aaccatgaac ctctggcggg ggaagacgac gatacgcttc tggttcccgt
    30661 ctctatacaa gggcgcgtaa caggcaacag cggcgttgat attttcgcct aacgtcagag
    30721 gtagcaccgt aatccgcgtc ttttccccgc tttgttgcgc tcaagacgca ggataattag
    30781 ccgataagca gtagcgacac aggaagaccg caacacatga ctgattacgc gataagcaag
    30841 aaaagcaagc gatcgctttg gatcccgatt ctggtattca ttaccctcgc ggcctgtgcc
    30901 agcgcaggtt acagctactg gcattcgcat caggttgccg ctgacgacaa agcgcagcaa
    30961 cgcgtcgtac cctccccggt cttctacgcg ctggatacct tcacggtcaa tctgggcgat
    31021 gcggatcgcg tgctttatat cggcataacc ctgcgcctga aagatgaagc tacccgctcg
    31081 cggctgagtg agtatttgcc ggaagtccgt agccgtttgc tgttactgtt ttcgcgtcag
    31141 gatgctgccg tactggcgac agaagaaggc aagaaaaacc tgattgccga gattaaaacc
    31201 acgctttcca ccccgcttgt tgccgggcaa ccgaaacagg atgtcaccga cgtgctgtat
    31261 accgctttta ttctgcgata acgacatggg cgatagtatt ctttctcaag ctgaaattga
    31321 tgcgctgttg aatggtgaca gcgaagtcaa agacgaaccg acagccagtg ttagcggcga
    31381 aagtgacatt cgtccgtacg atccgaatac ccaacgacgg gttgtgcgcg aacgtttgca
    31441 ggcgctggaa atcattaatg agcgctttgc ccgccatttt cgtatggggc tgttcaacct
    31501 gctgcgtcgt agcccggata taaccgtcgg ggccatccgc attcagccgt accatgaatt
    31561 tgcccgcaac ctgccggtgc cgaccaacct gaaccttatc catctgaaac cgctgcgcgg
    31621 cacagggctg gtggtgtttt caccgagtct ggtgtttatc gccgtggata acctgtttgg
    31681 cggcgatgga cgcttcccga ccaaagtgga aggtcgcgag tttacccata ccgaacagcg
    31741 cgtcatcaac cgcatgttaa agctggcgct tgaaggctat agcgacgcct ggaaggcgat
    31801 taatccgctg gaagttgagt acgtgcgttc ggaaatgcag gtgaaattta ccaatatcac
    31861 cacctcgccg aacgacattg tggttaacac gccgttccat gtggagattg gcaacctgac
    31921 cggcgaattt aatatctgcc tgcctttcag catgatcgag ccgctacggg aattgttggt
    31981 taacccgccg ctggaaaact cgcgtaatga agatcagaac tggcgcgata acctggtgcg
    32041 ccaggtgcag cattcacagc tggagctggt cgccaacttt gccgatatct cgctacgcct
    32101 gtcgcagatt ttaaaactga aacccggtga cgtcctgccg atagaaaaac ccgaccgcat
    32161 catcgcccat gttgacggcg tcccggtgct gaccagtcag tacggcactc tcaacggtca
    32221 gtatgcgtta cggatagaac atttgattaa cccgatttta aattctctga acgaggaaca
    32281 gcccaaatga gtgacatgaa taatccggcc gatgacaaca acggcgcaat ggacgatctg
    32341 tgggctgaag ctttgagcga acaaaaatca accagcagca aaagcgctgc cgacgcggtg
    32401 ttccagcaat ttggcggtgg tgatgtcagc ggaacgttgc aggatatcga cctgattatg
    32461 gatattccgg tcaagctgac cgtcgagctg ggccgtacgc ggatgaccat caaagagctg
    32521 ttgcgtctga cgcaagggtc cgtcgtggcg ctggacggtc tggcgggcga accactggat
    32581 attctgatca acggttattt aattgcccag ggcgaagtgg tggtcgttgc cgataaatat
    32641 ggcgtgcgga tcaccgatat cattactccg tctgagcgaa tgcgccgcct gagccgttag
    32701 tgatgaataa ccacgctact gtgcaatctt ccgcgccggt ttctgctgcg ccactgctgc
    32761 aggtgagcgg cgcactcatc gccattattg ccctgatcct cgctgctgcc tggctggtaa
    32821 aacggttggg atttgtccct aaacgcactg gcgttaacgg tctgaaaatt agcgccagtg
    32881 cttcgctggg cgcgcgtgaa cgggttgtgg tggtcgatgt ggaagatgca cggctggtgc
    32941 tcggcgttac cgcagggcaa atcaatctgc tgcataaact tcccccttca gcaccaacgg
    33001 aagagatacc gcagcccgat tttcagtcgg ttatgaaaaa cttgcttaag cgtagcggga
    33061 gatcctgatg cgtcgtttgt tgtctgtcgc acctgtcctt ctctggctgg ttacgcccct
    33121 cgccttcgcg caactgccgg gtatcaccag ccagccgctg cctggcggtg gacaaagctg
    33181 gtcgctcccg gtgcaaacgc tggtgttcat cacctcgttg acgtttattc cggcaatttt
    33241 actgatgatg accagtttca cccgcatcat cattgttttt ggtttattgc gtaacgcgtt
    33301 gggaacaccc tccgcgccac ctaaccaggt attgctggga ctggcgctgt ttttgacctt
    33361 ttttattatg tcgccggtga tcgataaaat ttatgtcgat gcgtaccagc cattcagcga
    33421 agagaaaatc tcaatgcagg aggcgctgga aaaaggggct cagccgctgc gtgagtttat
    33481 gctgcgtcag acccgcgagg cggatttagg gttgtttgcc agactggcga atactggccc
    33541 gttgcaggga cctgaagctg tgccgatgcg cattttgctc ccggcctacg tgaccagcga
    33601 gttgaaaacc gcatttcaga taggcttcac gattttcatt ccttttttga ttatcgacct
    33661 ggtgattgcc agcgtgttga tggcattggg gatgatgatg gttcccccag ccacaattgc
    33721 tctgcccttt aaactgatgc tgtttgtact ggtggatggc tggcaattgc tggtcggttc
    33781 gctggcgcag agcttttaca gctagagagg caaaatgaca cctgaatcgg tcatgatgat
    33841 ggggactgaa gcgatgaaag tcgcgctggc actggctgcc ccactgttgc tggtggcgct
    33901 ggtcacgggt cttatcatca gtattttgca ggccgccacc cagattaacg aaatgacgct
    33961 gtcgtttatt ccgaaaatca tcgccgtatt tatcgccatt attattgccg gaccgtggat
    34021 gctcaatctg ttgctggatt acgtccgcac cttgttcact aacctgccgt atatcatcgg
    34081 gtaggccgta caatgatgca ggtaacaagc gatcaatggc tttcctggtt aagcctgtac
    34141 ttctggccgt tactgcgcgt gctggcgctc atttctaccg caccaattct gagcgaacgc
    34201 agcgtgccga aacgggtaaa actgggtctg gcaatgatga tcacgttcgc tattgcccca
    34261 tcattacctg ccaacgatgt tcctgttttt tcgttctttg ctctgtggct ggccgtgcag
    34321 cagatcctga tcggcattgc gcttggtttt accatgcaat ttgcctttgc cgctgtgcga
    34381 accgctggtg aaattatcgg tctgcaaatg gggctgtcat ttgcgacgtt tgtcgatccg
    34441 gccagccatc tcaatatgcc cgttttagcg cgtatcatgg atatgctggc gttactgctg
    34501 ttcctgacat ttaacggtca tttatggttg atttcactgc tggccgatac ctttcacacc
    34561 ctgccaattg gtggcgaacc gttgaacagc aatgcgtttc tggcactcac caaagcaggg
    34621 agtttgattt tccttaacgg gctgatgctg gcattaccgc tcattactct gctgctgacc
    34681 ctgaatctgg cattaggctt acttaatcgt atggccccgc aattatccat ttttgttatt
    34741 ggatttccat taattctgac tgtcggtatc tctttaatgg cggcattaat gccgttaatt
    34801 gcgccttttt gcgaacattt attcagtgag atgtttaatt tgctggctga tattattagt
    34861 gaattgccat taatataatt ccgtaacgtt tatcatgtta tcctaaggat tatccgaaaa
    34921 ataataccta cgaacatctt ccaggatact cctgcggcga aatatttgtt ttaagctcac
    34981 tcacatatcg caacatttac tttactttaa gacaattcca ggcaaattat acaacacttt
    35041 acgggatagt aagtccgcct gaaaaatcgc gagagtggcg cattaggtga cccatgttgt
    35101 tccgtttagt catgatgaaa tattcaggta aggggaatta tcgttacgca ttgagtgagg
    35161 gtatgccatg tcaacgatta ttatggattt atgtagttac acccgactag gtttaaccgg
    35221 gtatctgttg agtagagggg ttaaaaaaag agaaatcaac gacattgaaa ccgttgatga
    35281 ccttgccata gcttgtgatt cacagcgccc ttcagtggtg tttattaatg aggactgttt
    35341 catccacgat gcttctaaca gtcagcgtat caagctcatc attaatcaac atcccaatac
    35401 gttatttatc gtttttatgg caattgccaa tgttcatttt gatgaatatc tattggtcag
    35461 aaaaaattta ttgatcagtt ctaaatcgat taaaccggaa tctctcgacg atatccttgg
    35521 cgatattctg aaaaaagaga caacgataac ctcgttttta aatatgccga cgttatcatt
    35581 gagccgaacc gaatcgagta tgttgcgaat gtggatggca ggtcagggaa ccattcaaat
    35641 ctctgaccaa atgaatatca aagccaagac cgtttcatcg cataaaggta atattaaacg
    35701 taagatcaaa acgcataata aacaggttat ctaccatgtc gtccgactga cggataatgt
    35761 gactaatggt atttttgtca acatgcgcta acacattctg actggtggtt tcccaccagt
    35821 caggccgaat tagattactc tgctttctcc acaaagatac cgtcctgatg ccctgcttca
    35881 ttaaagaacc agatgccgag cgggtagtct tccagcgaaa ccaggtacat tgtgccttca
    35941 ctaaactcct caactgccag taccacgcca ggacgacgcg gaccgccatc cgttttgact
    36001 gttacccgat cattcacctt catcgttttc ctcctgtggc tttgtgccag tgtagaacaa
    36061 tttcgttttt ctggcagcgc caggcgcgcg cgagtgctga ttttctcgac ggtctatact
    36121 taagagatgc caggcggact taacgactgg ccgcaacaac agagtaacgg ttgcgaggaa
    36181 agatgatgaa aaccgcaaaa gagtacagcg ataccgcaaa acgtgaggtc agcgtcgatg
    36241 tcgatgccct gctggcggcg atcaatgaaa ttagcgaaag cgaagttcat cgcagccaga
    36301 acgattctga acacgttagc gtcgatggac gtgaatatca tacatggcgt gaattggcgg
    36361 atgccttcga actggatatt catgacttca gcgtctctga agtgaatcgt tgaatgcaca
    36421 ataaaaaaat cccgaccctg agggggtcgg gatgaaactt gcttaagcaa gaagcactta
    36481 aaaaattcgt tacaccagga aatctgatgt gttcatcacc ttatccgcaa tttttttcgc
    36541 tgacaagaaa atattcgtga tatgaatgat taacgctact tatgaataga aatgtgacgc
    36601 gcgtcacccc ttggtggctc tggcgtcaac gggatcgctc ccgaaaaaat catctttgat
    36661 aatttgtgct gttttgagaa tcatcctggt aaggggagta ctgcgcaacc atgctttcaa
    36721 ttcaacaacc actactggtt tttagcgatc ttgatggcac cctgctggac agtcatagtt
    36781 atgactggca accagctgcc ccctggctca gccgtttacg cgaagcgaat gttcccgtca
    36841 ttctctgtag cagtaagaca tcagccgaaa tgctgtactt gcaaaaaacg ttggggctac
    36901 aaggtttacc gctgattgcc gaaaatggcg cagtgatcca gcttgctgag caatggcagg
    36961 atatagacgg ttttccacgc atcatctcag gtattagcca tggcgaaatc agccaggttt
    37021 taaatacgct acgcgagaaa gaacatttta aattcacaac ttttgatgat gtcgacgatg
    37081 caaccatcgc cgaatggacg ggattaagcc gtagccaggc ggcgctgacg cagctacatg
    37141 aggcgtcggt aacgctaatc tggcgcgaca gtgacgagcg tatggcacaa tttaccgctc
    37201 gtctgaacga actgggcttg cagtttatgc agggtgcgcg cttctggcac gtcctggatg
    37261 cctctgccgg aaaagatcag gctgccaact ggattatcgc gacctatcaa caatcgtcag
    37321 gcaaacgccc aaccacactt ggcctgggcg atgggccaaa cgatgcgccc ttactggagg
    37381 taatggatta cgcggtgatt gtgaaagggt taaaccgtga aggggtgcat ctgcatgatg
    37441 aggatccggc ccgcgtctgg cgaacgcagc gtgaaggacc ggaaggctgg cgtgaagggc
    37501 tggaccattt tttctccgcc cattaagcgt tatcgctcgc gaatacccga ttgcgcccgg
    37561 cctgtttagc gagataaagt cgacggtcgg ccagtgactg gagttgttca aaatcataat
    37621 caccggtttc ctcgctgctg cttaccccca gcgaggcact gatgcgtatc gtcgtactct
    37681 tggcgatcaa catctctttt tcatttagct tcaggcgaat acgttctgca acctccgcag
    37741 cctgcgtcag gctcgcgcct ggcagaatca cacaaaactc ctcaccaccg acccgcccgg
    37801 caacatcctg cgctcgcaag gaactgctaa ttaatccggc agcatgagaa agaacacggt
    37861 cgcccgcctg atgaccaaag cggtcattaa tcgctttaaa atggtcaagg tcgacctgaa
    37921 tgacagaaaa aggatgttgg tgcgtctgac acaatttagc gagcggacgg gctttttcga
    37981 acagtgcgcc acggttatat aaacgcgtta aggtgtcgtg ccacgcctgc cagtgcaacg
    38041 agctttgcaa aacatacatg ttgctgacca tccggcgaat cacataccag gagatgagta
    38101 acatggtggt aaagagcgcc cacagcaggg ttaatgcaat gctgatactg ccgaaatcgc
    38161 cgcgcacgcc ttcgcttagc gtatggacgc gcaccagcac accgtcgaaa tggtccagac
    38221 gttcccagct aacatagcga ctgttcatac gaatgccgcc ccgcgtgtca tgttccattg
    38281 cctgcgccag caaggccagt tcacgaggat caaaaatatt ccctgttgga tggtcaggat
    38341 tggaagaggt caataatctc agcttactgt catagagctg atactcacca tcgaggtttt
    38401 tatcgatggc gtttcttaaa aattgctgca tggtacgcac gggaatactc atccccagca
    38461 cgccatacca gtagttttca ctgtctaccg gaacactgac ggtaacctgc ggttcagtat
    38521 tgctggcgtg ttccggttgc gaagtgaacc agcgtaccgc gcggtgacga ttttctcgtt
    38581 gcgaatggcc gacaaaccag ggttgggtga catagccgta ataacgcgtt ggtacattgc
    38641 gcgtaaacaa ggtcggctgc gtcgaaacgt aaaatccggc acgcgagaca tacatcgcct
    38701 gttcaaccat cgacgaggag ttgtgtgcca gtcgcagcaa gtaaccaact tccagtgcag
    38761 cggtaatttc attgtcgagg ctttcatttt cgcgagacag gagattcccc tcgctgacta
    38821 atgcatccga cacaccattg actggcaggg tgcgtcgtcg gttgagttcg atttgccagg
    38881 cgtgctcgtc gcgatgctgt tcgaactcgg ttacggcatt acgcagagag gtgaaatcca
    38941 gtggcgctac gagagcttcg cgcatgccat tacgcaaaaa gatcagtttg tcgacgttat
    39001 actgcaaatg cttatcgagc gcgttggcta cgttttccag atgattacgc tggctggaga
    39061 tataggcatc ttccagcacc accacttcac gccaggtgag caaggtggaa aaaagcaata
    39121 ccacgataaa gcagagatta acgacatgac caggccccag gcggcgtgcg agttttttca
    39181 accagctctg gttttccatt tttgtctcgt gctgcaccct gacgactccc aacctgcttt
    39241 tttatgattc tggcataacc tggcgatagc gtaccgcaaa acagcgataa ttttataatg
    39301 gtggcatgat aagacgcgac agacatcgat agttttataa cgtaggcatg ataagacgcg
    39361 atagcgtcgc atcaggcaat tcagtgcaga cactggcaca cccgaccgga tgcgacgtga
    39421 gcgccacatc cggtcgggtg ttaatgatac cctttcaaac ttcttccgca gaacgactcc
    39481 cctcccccgg aacaagctcg gtttcatgaa aagcttcccg cttgaccccg taaccgtcat
    39541 accaacgaca ctcaaccata ccgctggagt atccggtgac aatcatccgc gggccgccct
    39601 ctttaaccgt aacttcctca ctaaccataa agctcatact cgcctccttt tttcgagtga
    39661 aacttgttca ccttagttga agatggcgaa ttttgcataa tagcctgtgc gagtattaat
    39721 gtgcctgacc gcgtattttc gctacggctt tcactcccag caccacgatg ccgccgatga
    39781 taaatccaag aatcagattt aaaacagtcg gtaatatcat cgccaccact gcactttgct
    39841 gcccggcgaa atgttcaatg gcgtgatgca gcggcgcaat tccatgtacc acaatcccac
    39901 cgccgacgag gaacatcgcc agcgtgccga caatcgataa cgccttcatc aaccagggcg
    39961 caatgatcaa taaccctttg cctaatgcct gcatcagcgc gctggatttt tccgccagcc
    40021 aataccccag gtcatcaatc ttaacgataa cccctaccag accgtagacg cccacggtca
    40081 ctaccagcgc gatgcctgaa agcaccagca cctgattaag caacggcgct tcggccacaa
    40141 tccccagcgt gatggcgacg atttccgcag acaatataaa atcggtacga atcgcccctt
    40201 ttattttgtc tttttcaaac ttcagcggat cctgcgccgc cagcttctcc agacgctgct
    40261 gactctgcgc cggatcttct ttatgtttac gcgcctccag catatgcagc actttctcta
    40321 ccccttcaaa gcagagaaac gcgccgccaa tcatcaacag tggcgtaatc gcccacggga
    40381 taaacgcact gatgatcagc gccagcggca ccagaatcac tttattgatc agcgatcctt
    40441 tcgccacgcc ccagaccacg ggaagttccc ggttggcgcg cacgcctgca acttgttgcg
    40501 cattgagcga taagtcatcc cctaatacac cggcggtttt cttcgccgcc agtttgccca
    40561 tcacggagat atcgtccagc agtgtggcga tatcatcgag caacgtcagt aaactactcc
    40621 cggccaaaag cactccctct catttttatt gttgaagggt gtaagtatga agcaaaaacg
    40681 gcagctctga tacttgcttc acaggtcaac atttatttaa caatcaagtg caattatatc
    40741 tctcgccagc agaatgattt tcacgtttac tatgaaccac ctttttattt cagccgtgag
    40801 ggattatgcg tttccgccag ttgttaccgc tttttggcgc gctgtttgcg ttgtatatca
    40861 tttggggctc aacctatttt gtcattcgga ttggcgtgga aagctggcct ccgttaatga
    40921 tggcgggcgt tcgattcctg gcagccggta ttttattgct ggcatttttg ctactgcgcg
    40981 gacacaaact tcccccgcta cgtccgctgc tcaatgccgc gctgattggc ctgttattgc
    41041 tggctgtcgg taatggcatg gtgacggttg ccgaacatca aaatgttcct tccggcatcg
    41101 ccgccgttgt ggttgcaacc gtgcccctct ttaccctgtg cttcagccgc ctgtttggca
    41161 ttaaaacgcg caaactggaa tgggtgggta tcgccattgg gcttgccgga atcatcatgc
    41221 tcaatagcgg tggaaattta agcggcaatc cgtggggcgc gattctgatt ttaatcggct
    41281 cgattagctg ggcgtttggc tcggtttatg gctcgcgcat taccttacct gtagggatga
    41341 tggcgggtgc gattgagatg ctggcggctg gcgtggtgtt aatgattgcg tcgatgattg
    41401 cgggtgaaaa actgacggcg ctcccttccc tttcaggctt ccttgcggtc ggctatctgg
    41461 cgctgtttgg ttcgattatc gccattaacg cttatatgta tttaatccgc aacgtcagtc
    41521 cggctctcgc caccagctac gcctacgtta acccagtggt cgcggtcttg ctgggtacgg
    41581 ggctgggtgg cgaaacgctg tcgaagattg aatggctggc gctcggcgta attgttttcg
    41641 cggtggtact ggtcacgttg ggaaaatatc tcttcccggc caaacccgtt gtcgcgccag
    41701 ttattccgga tgcatcaagc gagtaaatga atcccctgcg tgtcgatctg cgcgctggca
    41761 ccttcgccgc agatccactc ttccagacgc tcggtaagcg ccgcatccgt cagcttctcg
    41821 cgcccgcgta acgcacattc ccagacaatc aatacgcgcc aaccgagttc ctgcaaatgg
    41881 ctgatatcac gacgatcgcg ctcgacattt ttacctatct tctccagcca aaaatctgtt
    41941 cgcgtggcgg ggactttaaa caggtagcaa tggtgatggt gccagaagca accgtgggca
    42001 aatatcacac agcgaaattc atcaaggaca aaatccgggc gtccgggcag actggcgtcc
    42061 tgaacgcgaa acgccaggcc ctgcccagtt aacaaactgg cgaggcgctt ctcaatcgcc
    42121 gtatcacgcg tggcaatcgc gcgcatattt ttgctgcggg tggccttatc gtgaacgtcg
    42181 accatgttgt gcctcttgct gacgcaacgc caccgcctgt ttgatttttg gctcaagcag
    42241 ttttgccacc gcggcaaaga ccggcacgac caccgagtta ccgaactggc gataagcctg
    42301 agtgtccgaa accggaatgc ggaatttcgc ttctcccggc gcttcaaaac ccattaagcg
    42361 cgcacattcc cgaggcgtta accgacgtgg gcgatgttgc tgattcagcg gatcgtcaaa
    42421 gtctttctca cccgttgcca tatcccagcc gcgatcgatt aaaatttccg cgccatcttt
    42481 gtaataacgc gcagacagcg tacgcgtgac gctttgcgga ttgttcggat aaaccattcc
    42541 ataaccgaag ccgttaccgc gcgcctgatg ttttttcgca tatcgataga ggtacttcca
    42601 cagtaccggc gtcaggatat atttcgcctc gaccatcggg tccagcagct gcgccagcgt
    42661 cactcgctgt gcagggaaac atttgctgat atcacgcaga gtaaaatcgg ctttcagatt
    42721 cagatcgcga cgaaaaccca ccagcacgat gcgttcacgg tgctgcggca gaaaatgttt
    42781 gccgtcgatg attttcggat cgtccggccc gttatcttct gcatcagcca cgtcatagcc
    42841 cagttcgtcc agcgtctgca tgatgatgcg gaacgtttta ccctggtcgt gacttttcag
    42901 gtttttgacg ttttcgagca caaacatcgc cggacgacgc gcgtcgataa tgcgcaccac
    42961 atcaaagaac agcgtgccct gggtatcgca ggcaaaaccg tgcgcccgcc cgagcgagtt
    43021 ctttttcgat acgccagcca gcgaaaatgg ctgacaaggg aaaccggcca gtaaaacatc
    43081 gtgttcagga atgtgttgac gaatatgttc cgccgccgcc tcatcactca cgccttcttg
    43141 atggctgagg gtgatgtcgc ggatatcctc attaaaatga tgcgtcgccg ggtcgcaata
    43201 atggttggct ttataagtgc gtaccgcatg tttgttccat tcgctggtaa acacgcactg
    43261 tccaccaatc gattcaaaac cgcgacggat gccgccaatt ccggcaaata gatcgataaa
    43321 gcgaaacgca taatgcggat gatgtgccgg tggtttgggt aacaacgttt gcagatgggc
    43381 gaactctttc tcacttaaac ggtgccatgc cgagtcattc gccagcgcac gttttaaaat
    43441 tgccgcgctc cagtgattct cacccacacc attaagctgc gccaccagcg ttttaacatc
    43501 ataaatttgc agcagtttct ccagcattgc ctgtgcggca ttcccggtgc tgtatgaatc
    43561 ggttactgat atattttcct gcatagattt aaccggccat ctgagatggc cgacaggtta
    43621 acataattac tgccgtttag ggccacgcgc tcagccagca ggtgaaaacg catctatcac
    43681 cttgtgatcg ataccttcat tctcccccgc cagttcggca ccgagcttcg ccataaactc
    43741 gactaaaaat tgcgcgttgt gctgtgccag ctgcttgcct cttgcggttt gcatggtttg
    43801 cggtaatttc agtaactttg tctggaaatg atccagcgcg tagcgtttat catcaagcgg
    43861 gcgatgctgt gcgaacggat cttcgccatc aaacagcgcc aagcccaatg cccctgaaac
    43921 ggcaaatacg cgcgccaggc caatcgcccc cagggcttcc agccgatccg catcctggac
    43981 gattttagct tccgttgtta agggggcgat ttgcgcgctg aaactgtgag cggcaatggc
    44041 atgacaaacg gcctcaattt tctccgccgg aaattgcacg aactcttcgg taatgctgcc
    44101 aacttactga tttagtgtat gatggtgatt ttaaggtgct tgcgtggctt ccatttccat
    44161 cagatgtcct tcctgctccg ctactgaagg cgtggtgcgt aacggcaaaa gcactgccgg
    44221 acatcagcgc tatctctgct ctcattgccg taaaacatgg caactacagt tcacttacac
    44281 cgcct
//
LOCUS       DAZGOB010000027        43283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGOB010000027 DAZGOB010000000
VERSION     DAZGOB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.027"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHLIF_001551"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHLIF_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO9605585.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHLIF_001552"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHLIF_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO9605586.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHLIF_001553"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHLIF_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO9605587.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLSVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHLIF_001554"
     CDS             complement(1087..1581)
                     /locus_tag="ACHLIF_001554"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO9605588.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHLIF_001555"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHLIF_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO9605589.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHLIF_001556"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHLIF_001556"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO9605590.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHLIF_001557"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHLIF_001557"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO9605591.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHLIF_001558"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHLIF_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO9605592.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHLIF_001559"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHLIF_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO9605593.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHLIF_001560"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHLIF_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO9605594.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHLIF_001561"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHLIF_001561"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO9605595.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHLIF_001562"
     CDS             complement(9275..9769)
                     /locus_tag="ACHLIF_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO9605596.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIINVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHLIF_001563"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHLIF_001563"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO9605597.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHLIF_001564"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHLIF_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO9605598.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHLIF_001565"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHLIF_001565"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO9605599.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="ACHLIF_001566"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="ACHLIF_001566"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO9605600.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /gene="yghB"
                     /locus_tag="ACHLIF_001567"
     CDS             13861..14520
                     /gene="yghB"
                     /locus_tag="ACHLIF_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO9605601.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHLIF_001568"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHLIF_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO9605602.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHLIF_001569"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHLIF_001569"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO9605603.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHLIF_001570"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHLIF_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO9605604.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="ACHLIF_001571"
     CDS             17948..18874
                     /locus_tag="ACHLIF_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO9605605.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHLIF_001572"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHLIF_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO9605606.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="ACHLIF_001573"
     CDS             complement(19225..21444)
                     /locus_tag="ACHLIF_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO9605607.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHLIF_001574"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHLIF_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO9605608.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHLIF_001575"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHLIF_001575"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO9605609.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHLIF_001576"
     CDS             complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHLIF_001576"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO9605610.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLEQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26816..27298)
                     /locus_tag="ACHLIF_001577"
     CDS             complement(26816..27298)
                     /locus_tag="ACHLIF_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO9605611.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPIMFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHLIF_001578"
     CDS             complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHLIF_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO9605612.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27895..28554
                     /gene="qseB"
                     /locus_tag="ACHLIF_001579"
     CDS             27895..28554
                     /gene="qseB"
                     /locus_tag="ACHLIF_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO9605613.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28551..29900
                     /gene="qseC"
                     /locus_tag="ACHLIF_001580"
     CDS             28551..29900
                     /gene="qseC"
                     /locus_tag="ACHLIF_001580"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO9605614.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29946..30278)
                     /locus_tag="ACHLIF_001581"
     CDS             complement(29946..30278)
                     /locus_tag="ACHLIF_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO9605615.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            30597..31178
                     /gene="mdaB"
                     /locus_tag="ACHLIF_001582"
     CDS             30597..31178
                     /gene="mdaB"
                     /locus_tag="ACHLIF_001582"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO9605616.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            31209..31523
                     /locus_tag="ACHLIF_001583"
     CDS             31209..31523
                     /locus_tag="ACHLIF_001583"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO9605617.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(31571..33463)
                     /gene="parE"
                     /locus_tag="ACHLIF_001584"
     CDS             complement(31571..33463)
                     /gene="parE"
                     /locus_tag="ACHLIF_001584"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO9605618.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(33492..34073)
                     /gene="yqiA"
                     /locus_tag="ACHLIF_001585"
     CDS             complement(33492..34073)
                     /gene="yqiA"
                     /locus_tag="ACHLIF_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO9605619.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(34073..34900)
                     /gene="cpdA"
                     /locus_tag="ACHLIF_001586"
     CDS             complement(34073..34900)
                     /gene="cpdA"
                     /locus_tag="ACHLIF_001586"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO9605620.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYRHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(34925..35347)
                     /locus_tag="ACHLIF_001587"
     CDS             complement(34925..35347)
                     /locus_tag="ACHLIF_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO9605621.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(35348..35977)
                     /gene="nudF"
                     /locus_tag="ACHLIF_001588"
     CDS             complement(35348..35977)
                     /gene="nudF"
                     /locus_tag="ACHLIF_001588"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO9605622.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            36182..37663
                     /gene="tolC"
                     /locus_tag="ACHLIF_001589"
     CDS             36182..37663
                     /gene="tolC"
                     /locus_tag="ACHLIF_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO9605623.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            37811..38482
                     /locus_tag="ACHLIF_001590"
     CDS             37811..38482
                     /locus_tag="ACHLIF_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO9605624.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            38488..39648
                     /locus_tag="ACHLIF_001591"
     CDS             38488..39648
                     /locus_tag="ACHLIF_001591"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO9605625.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(39686..40474)
                     /gene="ygiD"
                     /locus_tag="ACHLIF_001592"
     CDS             complement(39686..40474)
                     /gene="ygiD"
                     /locus_tag="ACHLIF_001592"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO9605626.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            40617..41390
                     /gene="zupT"
                     /locus_tag="ACHLIF_001593"
     CDS             40617..41390
                     /gene="zupT"
                     /locus_tag="ACHLIF_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO9605627.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(41448..41618)
                     /gene="yqiD"
                     /locus_tag="ACHLIF_001594"
     CDS             complement(41448..41618)
                     /gene="yqiD"
                     /locus_tag="ACHLIF_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO9605628.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(41879..42532)
                     /gene="ribB"
                     /locus_tag="ACHLIF_001595"
     CDS             complement(41879..42532)
                     /gene="ribB"
                     /locus_tag="ACHLIF_001595"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO9605629.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(42636..42784)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            42906..43196
                     /gene="ubiK"
                     /locus_tag="ACHLIF_001596"
     CDS             42906..43196
                     /gene="ubiK"
                     /locus_tag="ACHLIF_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO9605630.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        10771 a        11558 c        10811 g        10143 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcgacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca agcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagag
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tgacagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagaga
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tagtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgccagatt cacgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc
     1261 ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgtgttctt
     1381 tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt aatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt
     9061 gttgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg ttaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcggcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac atgttgcaat agatattgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 atccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aagtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tacgcagtgt ggtgccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttctattca agccgccacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa cgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc aataatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccccaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgtaca tcgggcaaca gttctgacaa actattttct ttaattttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggaggaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctgatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgacgc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gtattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 tacgcccgta aaaccaggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggtg atagaacaga aagagcagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga gaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gcggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 agcgtacagg actttatcgc ccttcacttt ctcgaaagaa ggcagcaata cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gcggctgcac ggttacagct ttcgcttcct gctttttcgg
    20641 cgcaacaggt ttgttatccg cgcacggcag atcttcgcca tacggatgcg ggattgggtc
    20701 gattttccct ggggtatcaa gacgagtaga atccacaccg ctccagccag gcagcgcctc
    20761 ttttacgata attgcggtat tacgcacatc gcggatttcg ctaattggct cgcccatcgc
    20821 cagacgatgc gctacttcca ccagcggacg ttcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgcgcggt
    20941 acggcgcaga ctagcctcaa taccgccaag gatcaccggc acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccggtcgc ttacccgcga cgttatccgg
    21061 tgtgtaggca tcgtcatgac gtaaacggcg atcggcggta tagcggttga tcatcgaatc
    21121 catgttgcca gcagtaacac caaaaaacag attcggttta cccagacgca taaagtcgtc
    21181 tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgcg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaacagtt gcccaatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acatcacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagacta
    21901 atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accaaaatgc tggatggctc aaagaagcca cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcaccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcggacc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacaggccc
    24001 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    24061 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    24121 actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg aatggtcagc
    24181 gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg cgcggcttct
    24241 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    24301 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    24361 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttacct
    24421 gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    24481 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    24541 ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    24601 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttgctctta
    24661 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    24721 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    24781 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    24841 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    24901 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagctcactc
    24961 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    25021 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    25081 tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac cagcaaacct
    25141 tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    25201 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    25261 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    25321 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    25381 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    25441 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggt
    25501 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    25561 ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat ttccgcttca
    25621 gtcggataat ccggcccctg cacgatatcc agcagctgct cgagcgtggt tttcggctgg
    25681 tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg aatatcggtc
    25741 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgagcaggc
    25801 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    25861 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    25921 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    25981 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    26041 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    26101 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttaaga
    26161 ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    26221 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    26281 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    26341 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    26401 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    26461 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    26521 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    26581 atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    26641 gcggcttgct catggatacc cccgagacgg cttacggcgc accggataat aaatttcgcg
    26701 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt
    26761 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    26821 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    26881 tgtagtgaaa cataatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    26941 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    27001 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    27061 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt
    27121 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    27181 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    27241 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    27301 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    27361 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    27421 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata
    27481 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    27541 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctacg
    27601 gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg
    27661 ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg
    27721 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    27781 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag
    27841 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    27901 attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa
    27961 atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    28021 gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    28081 ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat
    28141 gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    28201 tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag
    28261 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    28321 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    28381 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    28441 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    28501 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    28561 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    28621 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    28681 tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca
    28741 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    28801 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata
    28861 acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg caactggtgg
    28921 gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    28981 ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    29041 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctattgggtc
    29101 gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac cctgactcgg
    29161 aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt gagtcgctaa
    29221 atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg
    29281 cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc
    29341 tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat tacgggatcg
    29401 atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac tcactggata
    29461 accttcagga cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg gtgatggata
    29521 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    29581 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    29641 ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct gataatttca
    29701 tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt ggcgaacgct
    29761 tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc
    29821 gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa ggtggatttg
    29881 aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga
    29941 ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca aaccagacaa
    30001 ataccgagcg agaatacata tacccaaaac tcgaacattt cccgcataaa gagttttctt
    30061 aagataagaa taataagtgg cgtaagaaga aaaaatgctg caatcattgc ttgcaatata
    30121 cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc aagcatccag
    30181 tcatacttat tgaaagcaaa aaaagcatct attaaccaat aagtaagcat tatgtataac
    30241 agagcttcaa atattgtttg aattttatgc tttaacatat atcagtacgc tcataaaaat
    30301 ccatttataa ttatcaacat gacacgttga tattttcatc ttttacttat tcaaagccat
    30361 attttcaatc ccaaaaaaaa ccgaaccaaa cgtgtaaatt attccggtgc gagtaagaat
    30421 tatgaggaat ggctatcagt attgtcattt tcagaaaata tttatcctgc atcggtgagt
    30481 cagagcaaga tcagactttt gctaaattcg caaaagactt tgcacatttt gctaatttca
    30541 ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt taaaaaatga
    30601 gcaacatcct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt caactgaacg
    30661 acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat gtccgcatcg
    30721 ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc tgggctgatg
    30781 tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg aaaaaatata
    30841 ttgatgatgt attcaccgaa ggtcatggaa cgctgtatgc cagcgatggc cgtacccgca
    30901 aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa tatatgcttt
    30961 ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc ttccacggcg
    31021 ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt ggcatggaac
    31081 cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc cgctatactg
    31141 aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg ctttagaagg
    31201 agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc
    31261 aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc
    31321 acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct atggcaccgg
    31381 attctatcgt gatgattgag cagtgggaaa gcatcgcgca tcttgaagcg catctgcaaa
    31441 ccccgcacat gaaggcgtac agcgaagccg taaaaggcga cgtgctggag atgaatatcc
    31501 gcatcctgca acctgggatt taaacctgcc ttgtttgccc ggccatcctg accgggcaat
    31561 gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac cagttgcggc
    31621 gatcttccga gcgtttcttc gccagcagca tatccatcat cgcgtcagta cgctgatcgt
    31681 cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc gtggtttcac
    31741 gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc ggcttgcctt
    31801 tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc gcgtaataaa
    31861 cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg tgaccgtgtt
    31921 tcaccaacgc gcggaagtgt tttacgaaca aagcgcagag cagcgtggca atgtgcagac
    31981 catcagagtc cgcatccgcg aggatacaga ttttgccata acgaagctgg ctcagatcgt
    32041 cgctgtcagg atcgataccg atcgctaccg aaatatcatg cacttcctgc gaggccagca
    32101 cttcgtcgga agaaacttcc caggtgttaa ggatcttacc tttcagtggc atgatcgcct
    32161 gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct tccacaagga
    32221 acagttcggt acggttaaga tcctgcgcgg tacagtcagc cagtttgcca ggcagtgctg
    32281 ggccgctggt cagctttttg cgcaccactt ttttagccgc acgcatacgg cgctgggcgc
    32341 tggaaatcgc catctccgcc agcagctccg ccgcctgaac gttctggttc agccacagga
    32401 taaaggcatc tttcaccacg cctgaaacga atgccgcgca ttgacgcgaa gagagacgct
    32461 ctttggtctg cccggcaaac tgcggatcct gcattttcac cgacagaaca taggcgcagc
    32521 gatcccagat atcttccgcc gacagcttca cgccgcgcgg cagaatgttg cggtattcac
    32581 agaactcacg catcgcgtcc aacaggccct gacgcagacc attaacatgg gtaccgccct
    32641 gcatcgttgg gataaggttg acgtagcttt cggtcagcag ttcaccgcct tccggcagcc
    32701 acagtagcgc ccagtccaca gcttcagtat cgccagcgaa attaccgata aacggttttt
    32761 ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga ccgtcctgat
    32821 agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca acgccggggc
    32881 acagtactgc tttggccttc agcacatgcg tcaggcgtga aacagaaaaa cgcgggctgt
    32941 caaagaaggt ttcatccggc cagaagtgca cgctggtacc ggtattgcgt ttaccgcaag
    33001 tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg ttataaacct
    33061 gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc accgaaatcc
    33121 ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta ccgcctgcat
    33181 gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga atatccaccg
    33241 gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaag attacatcca
    33301 cgcgttttgc gtgacccgcc agtgcttcat cgacactgtt atcaataact tcttgcccca
    33361 aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc ggctcaagcc
    33421 cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa gttagcaatt
    33481 cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt gaaataatct
    33541 tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca ggaagcgtag
    33601 tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag cagccagatc
    33661 aaatccggcg cttccagcgg gtcaatctgc atgactttga gatcgtaaat atggcgtgac
    33721 tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata gtccgtcagc
    33781 agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa acattgtgac
    33841 aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga atcgccgcca
    33901 tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg cggcaactgc
    33961 ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa caagctcgct
    34021 tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga catcagtagc
    34081 cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc tcggtggtca
    34141 gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatggtg tccagcgtaa
    34201 agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc aggcggcgac
    34261 cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat ttgacgtgcg
    34321 gaaacttcgc cagcacggta tccagttcgc ccgcgttacg caggctgtgt tgatcaagcc
    34381 aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg cgttctggcg
    34441 catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca ccgtgcggca
    34501 cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata aacacccgct
    34561 tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg aaatcgtggt
    34621 tgccaggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg aaatgccgat
    34681 aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg tcgaattcgt
    34741 gctggtgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggta tttaccccta
    34801 acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa atcctgactc
    34861 tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct taggtttcac
    34921 gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc aaccagtccg
    34981 ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta ttaggataat
    35041 cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc accatcgcgt
    35101 catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg ggcgcagtct
    35161 gttctattgt caccagggta gtgtatcggg tcgattccac aatcgtcagc cgatattgtg
    35221 cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt aacaaacggc
    35281 gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca ggtgtgtaac
    35341 gcttcattta tgcccactca ttttttaacg cttgatgatg cagctgcagc cattgcaaag
    35401 cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat gcctgttccc
    35461 ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga ataccgcttg
    35521 cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg ccccccgggc
    35581 ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagtcccgcc tcttcaatcg
    35641 cttcgcgacg ggcaacatct tccacacttt caccctcttc tatcatcccg gcaaccatct
    35701 ccagtagcca gggggtttcg ctggtgtcgt acgcggcaat ccgaatctgc tcaatcagca
    35761 caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg cgctcaaaaa
    35821 tttcccgccg tacctcatga ctcatttgcc cgttgaatag acgatgacga aatctataaa
    35881 gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct acatcgtttt
    35941 tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg gcactaatag
    36001 tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat gtgttaaatt
    36061 gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg gattctgcta
    36121 gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac aaggaatgca
    36181 aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tctgggttca gttcgttgag
    36241 ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc cggaattgcg
    36301 taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc gcagtccatt
    36361 actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc gcgacgcgaa
    36421 cggcatcaac tctaacgcga ccagtgcgtc cctgcagtta actcaatcca tttttgatat
    36481 gtcgaaatgg cgtgcgttaa cgctgcagga aaaagcagca gggattcagg acgtcacata
    36541 tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca acgtgttgaa
    36601 tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc gtcaattaga
    36661 tcaaaccacc caacgtttta acgtgggcct ggtagcgata accgacgtgc agaacgcccg
    36721 cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgcaataacc ttgataacgc
    36781 ggtagagcag ctgcgccaga tcaccggtaa ctactatcca gaactggcag cgctgaatgt
    36841 cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag aagccgaaaa
    36901 acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg agcaaattcg
    36961 ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg ggatttctga
    37021 cacctcttat agcggttcga aaacccgtgg tgccgctggt acccagtatg acgacagcaa
    37081 tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg gcggaatggt
    37141 taactcgcag gtgaaacagg cacagtacaa ctttgtcggt gccagcgagc aactggaaag
    37201 cgcgcatcgt agcgtcgtgc aaaccgtacg ttcctccttc aacaacatta atgcatctat
    37261 cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat tagacgcgat
    37321 ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg caaccaccac
    37381 gctgtacaac gctaagcaag agctggcaaa tgcgcgttat aactacctga ttaatcagct
    37441 gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac tgaacaatgc
    37501 gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc cggaacagaa
    37561 tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc aaacatccgc
    37621 acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac gacggggctt
    37681 cggccccgtc tgaacgtaag gcaacgtaaa gatacgggtt atctgccgca ttcttccccc
    37741 ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc actggtcccg
    37801 gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca aaaactggag
    37861 cgcgcgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc tggctggctg
    37921 tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt cagctgcaaa
    37981 cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag aagccgaacg
    38041 tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg aaggtcagtg
    38101 ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg cccagcaatc
    38161 cagcgggagt ttctggatgc cgctgatggc cggttacatg atggggcgtc tgatgggcgg
    38221 cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca gcccggctta
    38281 cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag gccgcaccat
    38341 gaccgtaccg aagacagcca tggcaccaaa accggcgacc accactaccg ttacccgtgg
    38401 cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa ctggcacctc
    38461 ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga gcgcccggac
    38521 tggcgtgaga aagcccacga atacggtttc aattttcaca ccatgtacgg cgagccgtac
    38581 tggtgtgaag atgcttacta caagttgacc ctcgcccagg ttgaaaagct ggaagaagtc
    38641 accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc cagcgatgag
    38701 ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgcca gtcatggctg
    38761 acgcaccagc catcgcttta ttcgcgtctt gatctggcgt gggatggcac tggcgaacct
    38821 aaacttctgg aaaataacgc cgatacgcca acgtcactat acgaggcggc gttctttcag
    38881 tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag cgaccagttt
    38941 aacagtctgc aagaaaaact gatcgatcgc ttcgttgagc tgcgtgaaca gtatggcttc
    39001 cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac cattcagtat
    39061 ttgcaggact gcgcaacgga agctgaaatt gctactgagt tcctctacat cgatgatatc
    39121 gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc caacctgttc
    39181 aagctgtatc cgtgggaatt tatgttgcgt gagatgtttt ccaccaagct ggaggatgca
    39241 ggcgtacgct ggctggaacc ggcgtggaag agcattatct ccaacaaggc acttctaccg
    39301 ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt tgcggaagat
    39361 gatcatccgc aaatggaaaa atatgtggtt aaaccgatct tctcccgtga aggcgcaaac
    39421 gtgtcgatca ttgaaaacgg caaaaccatt gaagcagcgg aaggtccgta tggcgaagaa
    39481 gggatgattg ttcagcaatt ccacccgtta ccgaaattcg gcgacagcta tatgctgatt
    39541 ggtagctggc tggtgaacga tcaacccgcc ggaattggca ttcgtgaaga ccgtgcattg
    39601 atcacccagg atatgtctcg gttttatcca catatttttg ttgagtaatc aatgatgccg
    39661 gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata ctcaggctgc
    39721 ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac gcacctaaga
    39781 catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca ccttcatggt
    39841 cgaggtaatt caccagagga tgctgctcta ccgccccttg ccacgtcaga ttcgctttca
    39901 catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc cacttcactg
    39961 tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca tctcgcagcg
    40021 ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg tcgatactca
    40081 actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc caggaaccgt
    40141 ggtcaaagcc ccaggcttct ttatccagcg tcaccgggac cggtgctaac agctcaacca
    40201 gatgctgtgc cagcacaggc gaacccggag caggataatg cgtatcgtac agcgcctgcg
    40261 ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact cctgttccac
    40321 gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat gtcatcccca
    40381 acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga ctaccgtgac
    40441 ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact aaaggtgtca
    40501 ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa caatgatgat
    40561 gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag ttgtcgatgt
    40621 cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc gcgtttctcg
    40681 gcgttctcgg gcaaaaaccc tcgaaccgct tactggcgtt ttcgctagga tttgcggcgg
    40741 ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa
    40801 tgtcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctat tttggcctgg
    40861 accgcatgtt gccacatgcg catccgcagg atttaatgca aaaatcggtg cagccgttgc
    40921 caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg cataacttcc
    40981 cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga tttggcatcg
    41041 cactggccgt cgcgttgcac aatattcctg aaggtctggc ggttgcaggc ccggtttatg
    41101 cagcaacggg ttctaaacgc acggcaattc tgtgggcggg gatttctgga ttggcagaaa
    41161 ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccc gtggtcatgg
    41221 cggcaatcat ggcggcggtt gcaggaatta tggtggcgct ctcggttgat gaattaatgc
    41281 cgctcgccaa agagattgac cctaataata accccagcta tggcgtacta tgtggaatgt
    41341 cagtgatggg attcagttta gtgctgctac aaacggcggg aattggttaa aagcaacatc
    41401 agaagttggc cagcgcatgg acaacttctg ctagcttcac taaagcctta ttctaccttt
    41461 tcggctgtct cttctctcgt actgtttaag tatttgttcc gcgcttcaag cagtgcgtct
    41521 ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca ggaaataacc caccacaaca
    41581 agagcaatca atacaatcca gtaccaggcg ataaacatcc ttttaccctt aatatttaac
    41641 aatactaaac atttagcgca taaatttcac atatcctttt tcggatatat tcatatggtc
    41701 gcataataac ggaacaatta tatgattttg atcataatga aaaagaatgc ctgatattaa
    41761 tgaaattttc tcatagaaag agaagcggaa ttaacatctt gcatagcagg gcaaaataaa
    41821 aaccgaagcc agctacaact tcggtttctt gattaaggca gtaattaagc agcggttttc
    41881 agctggcttt acgctcatgt gcctgacggt atgccaccag gtcttcaata gtcacgagcg
    41941 ccatattgtg tttattggca aactcaatgc actctggtgc acgcgccatc gtgccatcgt
    42001 cattagtcaa ctcacacagc acaccagccg gtttaaagcc tgccagcgtc atcaaatcaa
    42061 tagtcgcttc agtatgaccg ccacgcgtca gtacaccacc tgcctgcgcg cgaagtggga
    42121 aaacgtggcc cgggcgattc agatcagaag gttttgcgcc atcggcaatc gctgcgcgaa
    42181 cggtcgtaat tcgatcagcg gcagaaacac cggtagtcac accttcagcc gcttcaatgg
    42241 tcacggtaaa accggtgcca taggcgctgg tgttattttc taccatcatt ggcagatcaa
    42301 gttgtttacg gcgatcttca gtgatgcaca ggcaaacaat accgctaccg tggcgaatgg
    42361 tcagcgccat ctgctcaacg gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt
    42421 cacggtcttc atcatcaagc accattacac cgcgtccttc acgcagcgca gccagtgcat
    42481 tttcaacacg ttcgaaaggc gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa
    42541 aacctcacta aaattatggt taccagaatc agggcagtct taggagtggc ggcatatagc
    42601 caaaataacg tgagcgggtc catgcccgac agaatcgtta ctctctccca tccggactct
    42661 aaccgtcggc cccggaatta caccggatct gctgtccttt gagttcgcac ccaaagcgct
    42721 cgcgggcttt caactgagtt gatttaccgc cggtggggaa tttcgccccg ccctgagaat
    42781 aagcggattc actataacgc taatgattag cggcagcaac gcatagcttc acataattct
    42841 ggtttatgac ttacccttat cgcactacaa tggcactcaa cacctatcag tacagggaaa
    42901 ctacaatgat tgacccgaaa aaaattgagc aaatcgctcg ccaggttcac gaatcaatgc
    42961 ctaaaggaat cagggagttc ggggaagatg tggagaaaaa aatccgccaa accctacaag
    43021 ctcagctgac gcgcctcgac cttgtaagcc gtgaagagtt cgacgtccaa acgcaagtcc
    43081 tgttacggac tcgtgagaaa ctggcgctgc ttgaacaaag aatgagcgaa ctggaaaatc
    43141 gcagtactga gattaaaaag cagccagatc cagagactct ccccccaacg ctgtaaacat
    43201 ttgtctttaa tgcactgtaa attatgaatt aatcctctaa tttacagtgc tttctattac
    43261 cttatcgctt aattccattc aga
//
LOCUS       DAZGOB010000028        41987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGOB010000028 DAZGOB010000000
VERSION     DAZGOB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.028"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            242..3127
                     /gene="ycgV"
                     /locus_tag="ACHLIF_001597"
     CDS             242..3127
                     /gene="ycgV"
                     /locus_tag="ACHLIF_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO9605631.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINEGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(3227..5146)
                     /gene="dhaR"
                     /locus_tag="ACHLIF_001598"
     CDS             complement(3227..5146)
                     /gene="dhaR"
                     /locus_tag="ACHLIF_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO9605632.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5374..6444
                     /gene="dhaK"
                     /locus_tag="ACHLIF_001599"
     CDS             5374..6444
                     /gene="dhaK"
                     /locus_tag="ACHLIF_001599"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO9605633.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6455..7087
                     /gene="dhaL"
                     /locus_tag="ACHLIF_001600"
     CDS             6455..7087
                     /gene="dhaL"
                     /locus_tag="ACHLIF_001600"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO9605634.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7098..8516
                     /gene="dhaM"
                     /locus_tag="ACHLIF_001601"
     CDS             7098..8516
                     /gene="dhaM"
                     /locus_tag="ACHLIF_001601"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO9605635.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            8836..10533
                     /gene="treA"
                     /locus_tag="ACHLIF_001602"
     CDS             8836..10533
                     /gene="treA"
                     /locus_tag="ACHLIF_001602"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO9605636.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFDLMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(10612..11052)
                     /gene="ycgY"
                     /locus_tag="ACHLIF_001603"
     CDS             complement(10612..11052)
                     /gene="ycgY"
                     /locus_tag="ACHLIF_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO9605637.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLAPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(11230..11484)
                     /gene="ymgE"
                     /locus_tag="ACHLIF_001604"
     CDS             complement(11230..11484)
                     /gene="ymgE"
                     /locus_tag="ACHLIF_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO9605638.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            11685..12419
                     /gene="ycgR"
                     /locus_tag="ACHLIF_001605"
     CDS             11685..12419
                     /gene="ycgR"
                     /locus_tag="ACHLIF_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO9605639.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(12421..13032)
                     /gene="emtA"
                     /locus_tag="ACHLIF_001606"
     CDS             complement(12421..13032)
                     /gene="emtA"
                     /locus_tag="ACHLIF_001606"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO9605640.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            13132..14046
                     /gene="ldcA"
                     /locus_tag="ACHLIF_001607"
     CDS             13132..14046
                     /gene="ldcA"
                     /locus_tag="ACHLIF_001607"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO9605641.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            14141..15877
                     /gene="cvrA"
                     /locus_tag="ACHLIF_001608"
     CDS             14141..15877
                     /gene="cvrA"
                     /locus_tag="ACHLIF_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO9605642.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPLRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16263..17333)
                     /gene="dadX"
                     /locus_tag="ACHLIF_001609"
     CDS             complement(16263..17333)
                     /gene="dadX"
                     /locus_tag="ACHLIF_001609"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO9605643.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(17343..18641)
                     /gene="dadA"
                     /locus_tag="ACHLIF_001610"
     CDS             complement(17343..18641)
                     /gene="dadA"
                     /locus_tag="ACHLIF_001610"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO9605644.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVHPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            18971..20503
                     /gene="ycgB"
                     /locus_tag="ACHLIF_001611"
     CDS             18971..20503
                     /gene="ycgB"
                     /locus_tag="ACHLIF_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO9605645.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(20555..21274)
                     /gene="fadR"
                     /locus_tag="ACHLIF_001612"
     CDS             complement(20555..21274)
                     /gene="fadR"
                     /locus_tag="ACHLIF_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO9605646.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            21496..23037
                     /gene="nhaB"
                     /locus_tag="ACHLIF_001613"
     CDS             21496..23037
                     /gene="nhaB"
                     /locus_tag="ACHLIF_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO9605647.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            23183..23713
                     /gene="dsbB"
                     /locus_tag="ACHLIF_001614"
     CDS             23183..23713
                     /gene="dsbB"
                     /locus_tag="ACHLIF_001614"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO9605648.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(23759..25027)
                     /gene="umuC"
                     /locus_tag="ACHLIF_001615"
     CDS             complement(23759..25027)
                     /gene="umuC"
                     /locus_tag="ACHLIF_001615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO9605649.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(25027..25446)
                     /gene="umuD"
                     /locus_tag="ACHLIF_001616"
     CDS             complement(25027..25446)
                     /gene="umuD"
                     /locus_tag="ACHLIF_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO9605650.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            25819..26730
                     /gene="hlyE"
                     /locus_tag="ACHLIF_001617"
     CDS             25819..26730
                     /gene="hlyE"
                     /locus_tag="ACHLIF_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO9605651.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(26937..27383)
                     /gene="ycgN"
                     /locus_tag="ACHLIF_001618"
     CDS             complement(26937..27383)
                     /gene="ycgN"
                     /locus_tag="ACHLIF_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO9605652.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLMGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(27475..28134)
                     /gene="ycgM"
                     /locus_tag="ACHLIF_001619"
     CDS             complement(27475..28134)
                     /gene="ycgM"
                     /locus_tag="ACHLIF_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO9605653.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(28206..28499)
                     /locus_tag="ACHLIF_001620"
     CDS             complement(28206..28499)
                     /locus_tag="ACHLIF_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO9605654.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(28511..28669)
                     /locus_tag="ACHLIF_001621"
     CDS             complement(28511..28669)
                     /locus_tag="ACHLIF_001621"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605655.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            28740..29141
                     /gene="pliG"
                     /locus_tag="ACHLIF_001622"
     CDS             28740..29141
                     /gene="pliG"
                     /locus_tag="ACHLIF_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO9605656.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(29244..29612)
                     /gene="ycgJ"
                     /locus_tag="ACHLIF_001623"
     CDS             complement(29244..29612)
                     /gene="ycgJ"
                     /locus_tag="ACHLIF_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO9605657.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDASGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            30132..30827
                     /gene="minC"
                     /locus_tag="ACHLIF_001624"
     CDS             30132..30827
                     /gene="minC"
                     /locus_tag="ACHLIF_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO9605658.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            30851..31663
                     /gene="minD"
                     /locus_tag="ACHLIF_001625"
     CDS             30851..31663
                     /gene="minD"
                     /locus_tag="ACHLIF_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO9605659.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            31667..31933
                     /gene="minE"
                     /locus_tag="ACHLIF_001626"
     CDS             31667..31933
                     /gene="minE"
                     /locus_tag="ACHLIF_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO9605660.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(32305..32649)
                     /locus_tag="ACHLIF_001627"
                     /pseudo
     CDS             complement(32305..32649)
                     /locus_tag="ACHLIF_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(32684..32803)
                     /locus_tag="ACHLIF_001628"
     CDS             complement(32684..32803)
                     /locus_tag="ACHLIF_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO9605661.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(32764..32949)
                     /gene="ymgJ"
                     /locus_tag="ACHLIF_001629"
     CDS             complement(32764..32949)
                     /gene="ymgJ"
                     /locus_tag="ACHLIF_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO9605662.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            33050..33223
                     /gene="ymgI"
                     /locus_tag="ACHLIF_001630"
     CDS             33050..33223
                     /gene="ymgI"
                     /locus_tag="ACHLIF_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO9605663.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            33225..33569
                     /gene="ymgG"
                     /locus_tag="ACHLIF_001631"
     CDS             33225..33569
                     /gene="ymgG"
                     /locus_tag="ACHLIF_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO9605664.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGIGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            33573..33908
                     /gene="ymgD"
                     /locus_tag="ACHLIF_001632"
     CDS             33573..33908
                     /gene="ymgD"
                     /locus_tag="ACHLIF_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO9605665.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(33965..36286)
                     /locus_tag="ACHLIF_001633"
     CDS             complement(33965..36286)
                     /locus_tag="ACHLIF_001633"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO9605666.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(36403..36612)
                     /locus_tag="ACHLIF_001634"
     CDS             complement(36403..36612)
                     /locus_tag="ACHLIF_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605667.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(37012..37230)
                     /gene="ymgF"
                     /locus_tag="ACHLIF_001635"
     CDS             complement(37012..37230)
                     /gene="ymgF"
                     /locus_tag="ACHLIF_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO9605668.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(37362..38885)
                     /gene="pdeG"
                     /locus_tag="ACHLIF_001636"
     CDS             complement(37362..38885)
                     /gene="pdeG"
                     /locus_tag="ACHLIF_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9605669.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETNEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(39217..39465)
                     /gene="ymgC"
                     /locus_tag="ACHLIF_001637"
     CDS             complement(39217..39465)
                     /gene="ymgC"
                     /locus_tag="ACHLIF_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO9605670.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(39578..39844)
                     /gene="ariR"
                     /locus_tag="ACHLIF_001638"
                     /gene_synonym="ymgB"
     CDS             complement(39578..39844)
                     /gene="ariR"
                     /locus_tag="ACHLIF_001638"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO9605671.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(39873..40145)
                     /gene="ymgA"
                     /locus_tag="ACHLIF_001639"
     CDS             complement(39873..40145)
                     /gene="ymgA"
                     /locus_tag="ACHLIF_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO9605672.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(40188..40424)
                     /gene="ycgZ"
                     /locus_tag="ACHLIF_001640"
     CDS             complement(40188..40424)
                     /gene="ycgZ"
                     /locus_tag="ACHLIF_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO9605673.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            40738..41949
                     /gene="bluF"
                     /locus_tag="ACHLIF_001641"
     CDS             40738..41949
                     /gene="bluF"
                     /locus_tag="ACHLIF_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO9605674.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT        11043 a        10325 c         9806 g        10813 t
ORIGIN      
        1 ttggaataag caacgacaac ttagggaata actcgatata aactcacctt tgtaaggggg
       61 atgttaaata tatattaatt cctgtgttcc tgattttgcc cggtaataga gaattattct
      121 ctgagaagct catcatgatt gcaccataca aatatcatga tgataagagg atattattca
      181 ttgatttaaa caaaatgaca aaatccatag acccatagtc tgggtactat ataggagtgg
      241 tatgggcatc aaacaacaca atgggaatac caaagcagat cgtctcgctg aattaaaaat
      301 ccgttcgccc tcaattcaac tgataaaatt tggcgctatt ggtttgaatg caattatctt
      361 ttcccccctg ctgatagctg ctgatacagg aagtcaatat ggcaccaata ttactattaa
      421 tgaaggtgac agaattacag gagataccgc cgatccatca ggaaacctct atggtgtaat
      481 gaccccagca ggaaacacgc ctggcaatat caacctgggt aatgatgtca ccgtcaatgt
      541 caacgacgcc tctggatatg caaaaggaat cattattcag ggcaaaaaca gctccctgac
      601 agctaaccga ctcacagtag atgttgttgg tcaaacctct gccatcggca ttaacttaat
      661 tggtgactat acccatgctg acttaggcac aggcagcacc attaagagta acgatgacgg
      721 catcattatt gggcatagct caacactaac agccactcaa ttcaccattg aaaactcgaa
      781 cggtataggc ctaaccatca atgactatgg caccagtgtc gatcttggaa gcggaagtaa
      841 aatcacgacc gatggaagta caggtgttta tatcggtggt ctcaacggca ataacgccaa
      901 tggtgctgcg cgttttacgg ctacagacct gacaatcgat gttcagggct acagcgccat
      961 ggggataaac gtacagaaaa actctgttgt cgatctcgga acaaacagtt ccattaaaac
     1021 caatggcgat aatgcacacg gcctctggag ctttggccag gttagcgcga atgcactcac
     1081 tgttgatgta actggagccg cggccaatgg cgtcgaagtt cgtggtggta caaccactat
     1141 cggtgcagat agccatattt cttccgcgca gggcggtggc ctcgtcacca gtggttcaga
     1201 cgcgacaatc aatttttctg gcacggcagc gcaacgaaac agcatctttt ccggcggttc
     1261 ttatggtgcc tcggcccaga cggcaacggc tgttgtcaac atgcaaaata ccgatattac
     1321 agttgatcgt aatggcagtc tggcgctggg tttgtgggcg ctcagcggcg gtagaataac
     1381 cggagacagt ttggctatca ccggcgcggc aggagccagg ggaatttatg ccatgaccaa
     1441 cagccagatc gacctcacga gcgatctggt cattgatatg agtacacccg accagatggc
     1501 cattgcaacg caacatgacg atggttatgc cgccagccgc atcaacgcct cgggtcgtat
     1561 gcttatcaac ggtagcgttc tttccaaagg tgggctaatc aatctagata tgcacccagg
     1621 gtcggtttgg acaggttcct ccctcagcga taatgtcaat ggcgggaaac tggacgttgc
     1681 aatgaataac agcgtctgga acgtaacaag taattctaat ctcgacacgc tggcgctgag
     1741 ccattcaact gtcgattttg ccagccacgg gtcaactgcc ggcacatttg ccacattaaa
     1801 cgtagagaac ctgagcggta acagtacctt tattatgcgt gctgatgttg ttggcgaggg
     1861 taatggcgtt aataataaag gggatttatt gaatatcagc ggaagtagtg ctggtaatca
     1921 cgtattggct atccgcaacc agggcagcga ggccactacg ggaaatgaag ttctgacagt
     1981 ggtaaaaacc actgacggtg cggcctcgtt cagcgcgtct tctcaggttg agttgggggg
     2041 atatctgtac gatgtgcgta aaaatggcac taactgggag ctttacgctt ccgggacagt
     2101 tccggaaccg actcctaatc ctgaaccaac accggctccc gctcagcctc ccatagtcaa
     2161 ccccgatcct acgcctgaac ccgatcctac gcctaacccc actcccacac ctaaacccac
     2221 cacgaccgca gatgctggcg gcaattatct caatgtcggt tacttattaa actatgttga
     2281 aaaccgtacg ctgatgcaac gaatgggtga cctgcgaaat cagagtaaag acggtaatat
     2341 ctggctgcgc agttatgggg gaagcctgga ctccttcgcc agtggcaaac tgagcggctt
     2401 tgacatgggt tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc
     2461 gttgtatgtc ggtctgtata ttggctcaac acatgcatcg ccggactata gcggaggcga
     2521 cggtaccgca cgttcagact acatgggaat gtatgccagt tacatggcac acaacggttt
     2581 ttacagcgat ctcgttgtaa aagcatcgcg ccagaaaaat agttttcacg tactggacag
     2641 tcagaacaac ggcgttaacg ccaacggcac tgcgaatgga ttgagcatct ccctggaagc
     2701 cgggcagagg ttcaacctga cccctactgg ttatgggttc tatatagagc cgcaaaccca
     2761 gctcacatac agccaccaga atgagatggc tatgaaggcg agtaatggtc tcaatataca
     2821 tctgaatcac tacgaatcgc tgctggggcg tgccagcatg atactgggat atgacatcac
     2881 cgcaggcaac agccagctga atatgtatgt gaagactgga gctatccgcg agttttcagg
     2941 ggatacagac tatctgttga acaactcccg ggagaagtac agtttcaaag gtaatggctg
     3001 gaataacggc gtgggagtca gtgcacagta taacaaacag cacacattct acctcgaagc
     3061 ggattacacg cagagtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag
     3121 cttttaagtg cgggatagac cagcccttaa acgatgctga aaaatgttag cgtgcgccgg
     3181 aatgaaatat agatcactct gatgtatcag aaatcgtctc tgtctttcat acccggcgct
     3241 taaactgccc tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga
     3301 tcccaagtaa agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa
     3361 ttgcctcttt ttcaacttcc gcaaatgaca gactggtgga aaggcgtgtg gcgctgacat
     3421 catctgttgc ctgctcggta aacagatgtt ccggcaaatc actgacgcga atgcgcccat
     3481 tatcactact cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgccaggcc
     3541 atgcacagga aaccaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc
     3601 gtttttcaag actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac
     3661 gcagaggcgg gatggtaatt tcaaatgcat gcaacgcgta atacagctgg cgactaaaac
     3721 gattttgttc caccagcatt gcgaggtccg cggtcgttgt ggcaatcact ttgacatcaa
     3781 ctggtattaa acgccgcgca tccagtcgcg tgataacacc ctgcttgata acctgaagca
     3841 aagcagactg taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt
     3901 gtgccagttc cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa
     3961 attcttccgc cagcgccgca tcaccatata actcacaatt gacggcgata taaggacctg
     4021 cagcacgctc gctttcatta tgaattgcct gacttagcag tgcctttccc acgccctctt
     4081 ctccacaaag caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc
     4141 ggcgggtttg cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt
     4201 gactggtcat caactgccgc atctgttcca caggatggag caacaaaata aagctggttc
     4261 cctgcgtttc tattatcggt ttaagggtta tcaccgcatc aataaactga tgctgacttt
     4321 caaaggttgc ttctacgtgt ttgagcggat gtgcctgttt tattgcttgt tgcaatacgg
     4381 cgggtaacgt taagagttca gtgattgccc ttccctgact tgccgtcgcg tcaaggcgca
     4441 agacccgcgc cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca
     4501 cgccatcatc catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag
     4561 cgagcaaact gtccgtcagc agtaaatttc cgacctcgcg ggcgattgcc aacgtcaacg
     4621 gcaaatcagc tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc
     4681 ggcccttgct gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga
     4741 aatgttgatc ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac
     4801 aagtaccaat aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct
     4861 gctgcaaggt ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa
     4921 acagcgcaca ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga
     4981 gcgtcagcat cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat
     5041 gtggtacgtt ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa
     5101 ttaagggaga tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt
     5161 atgacaaaag gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat
     5221 ggaacattca ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc
     5281 ccttgcctga tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca
     5341 tccatgccct accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa
     5401 gacgtactgg acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat
     5461 caggatccgg tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg
     5521 tcgggtggcg gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt
     5581 tcgggggcct gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc
     5641 tccatgcaaa ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat
     5701 attcttaact ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact
     5761 gtggtcattg atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc
     5821 gttgccaaca ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca
     5881 ctggacgcct gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc
     5941 gctctcggtg cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct ggcggataat
     6001 gagatggagt ttggcgtcgg cattcatggt gagccgggta ttgaccgccg ctccttctct
     6061 tcccttgatc aaaccgtcga tgaaatgttc gacaccctgc tggaaaatgg ctcataccat
     6121 cgcactttgc gtttctggga ttatcaacaa ggcagttggc aggaagaaca acaaaccaaa
     6181 caaccgctcc agtctggcga tcgggtgatt gcgctggtta acaatcttgg cgcaactccg
     6241 ctttctgagc tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg
     6301 actatcgaac gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca
     6361 atcaccttac tgaaagttga tgacgaaacg ctggcactct gggacgcccc ggtccacacc
     6421 ccggccctta actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt
     6481 taactggctc actcgttgtg gcgatatttt cagcaccgag agcgagtatc ttaccggact
     6541 ggatcgcgaa attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt
     6601 ggtggaaaaa ctccctgcta tcgcagataa agatatcggt ttcattctca agaataccgg
     6661 tatgacgctg ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat
     6721 ccgcgccgca caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat
     6781 gttccgcgat ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac
     6841 catgtgtgat gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct
     6901 ctctgttccg gtggcgctcg aagctgccag tagaatcgcc gaatccgctg cacaaagtac
     6961 gattacgatg caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca
     7021 ccaggatccc ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa
     7081 agagtaagga attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag
     7141 aaggtgtcgg tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg
     7201 ccgcgggaat tgacgatcca caaaatccta ttggtaccga tgccgtcaaa gtgatggagg
     7261 ccatcgaatc tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat
     7321 tattgagtgc tgaaactgcg ctggaattgc tggctcccaa gatcgccgca aaagtacgtt
     7381 tgtgtgctgc gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcgggag
     7441 cggatatcga caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac
     7501 tgggtttacc gtcctccgac actgaaatct ctgacacatg tcctgcgtac gatgaagaag
     7561 cccgttctct ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc
     7621 ggctggttta taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat
     7681 gcgtcacacc agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc
     7741 tgcgcctgat tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcagctgg
     7801 ctgaagataa ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttccgc
     7861 ctgtttcggg taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa
     7921 ccctgaccgt ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag
     7981 atctgatgac gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct
     8041 tttctggtca ccatacactg ttagatgatc cggaactgct ggcggcggca agcgaactcc
     8101 ttcagcatga acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc
     8161 agcaatacca gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc
     8221 ttctgcatcg caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact
     8281 cgccaactat tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg
     8341 cggttgtaaa aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg
     8401 cccgtgaact ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac
     8461 cagaagaaac gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca
     8521 ccgttcggat ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta
     8581 actacctgtt atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaacccaa
     8641 cgtcgctgac tggctaataa acagacgtta tttgatcact ctatttcacc gttatttatc
     8701 cctttaattc ccttttctaa aatgcctgac agttcgcaga atgagatttc gatcatgcag
     8761 ctagtgcgat cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc
     8821 aaaggagaat gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt
     8881 ccagcctgta tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta
     8941 acaccacagc cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgccaaa
     9001 ctttttccgg accaaaaaac ctttgccgat gccgtgccga acagcgatcc gctgatgatc
     9061 cttgctgatt atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac
     9121 gtcaatttca ccctgccgaa agagggcgag aaatatgttc cgccagaggg gcagtcactg
     9181 cgcgaacata ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa
     9241 tgggattctc tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag
     9301 gtatattact gggacagtta cttcaccatg ttaggacttg ccgaaagcgg tcactgggat
     9361 aaagtcgcgg atatggtggc caattttgct catgaaatag acacttacgg tcatattccc
     9421 aacggcaacc gcagttacta tttaagccgc tcgcaaccgc ccttctttga cctgatggta
     9481 gagttactgg cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa
     9541 aaagaatatg cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa
     9601 cgcgttgtaa aacttcagga tggtaccctt ctcaaccgct actgggacga tcgcgatacg
     9661 ccacgaccag agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct
     9721 gccactgaaa tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg
     9781 cgctggatgg acaacccgca tcaactaaat accttacgca ccaccagcat cgtaccggtc
     9841 gatctgaaca gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgcc
     9901 ggagataacg cgatggcaaa ccagtacgaa acgctggcaa atgcccgtca aaaagggatc
     9961 gaaaaataca tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat
    10021 aaagtgcgca atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg
    10081 aaagatcgcg ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc
    10141 ctgaacacca cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg
    10201 ttacagtggg tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac
    10261 attagctggc acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg
    10321 gaaaaatatg atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag
    10381 gatggctttg gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa
    10441 gagcaaccgt gtgacaatgt tccggcgacg cgtccgaccg ttaagtcagc aacgacgcaa
    10501 ccctcaacca aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc
    10561 ggcttctccg gatggcgttg tcggcagata acaccatagc tatggtgttt tctacacaga
    10621 cggtttttca agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa
    10681 gatagcgtca tttctacggg taagtgtttc actttcatca cccggtgatt tacaccgacg
    10741 ttgcctatat aaagcatcga ttgctacctg aatattgtta cggtgttcac tcatcaacgt
    10801 ttttttgctt tctggagcta aattaataat cacataatcc agacactcca gagaatttag
    10861 caaccaaaaa aagaattcag accattcctc ttcgcgtaac tgaagcattg ccgaacgggt
    10921 attttggctt aatctgccag aaggattgat caattctaaa tgttcaataa gcgcagccaa
    10981 aaccgcctca ctatccgccc catttttaac gcctgactgg ctactgtcta aaatatttat
    11041 tttctgaatc acaccaaaaa caacgaaaca ataattaaaa aaggattctg ttgttgttac
    11101 cgggaaccga ctttatcggg catctaagtg ctatattgat ttgttccacc cgtaaagcag
    11161 gtgtcgagta tacgttctga tgtgaaagcg gatagcggca tctgtatcgc tatccgcctt
    11221 atgaaaatct tattctcttc gcaggaggcg gaatacgccc agaacgagaa tagctcccac
    11281 caccgccacc aggaagctgt gcagattaaa accactgatg gagccgccaa tgccaaacat
    11341 ggtcgccagc cagccgccga ccaccgcacc gactatcccg agaatacagg tcaggaaaaa
    11401 tccaccacca tcacgccccg gcatgattag cttggcgata atgccggcta tcaggccaaa
    11461 aataatccag gcaataattc ccatgttcag gccctcgttt cgatgaatcg gacgcaagaa
    11521 atgcttacgt caacgctcta agtataggca agggcgggat cgatgctggg cgtatgtcgg
    11581 ctgatttaag aacaacatgt aaaaaatatt cacgcatttg tattaagttt tgttaactgt
    11641 gaccgataaa ccaaagacag tttgtcagtc aggagttttt ccgcgtgagt cattaccatg
    11701 agcagttcct gaaacaaaat ccgttagccg tcctgggcgt gttacgcgat ttgcacaaag
    11761 ccgcaattcc tttgcgtctc agttggaatg gcgggcagct gatcagcaaa atactggcaa
    11821 taaccccgga taaactggtg ctggatttcg gcagtcaagc cgaagacaac atcgccgtgc
    11881 taaaggcaca gcacattacc attaccgccg aaactcaggg tgcgaaagtc gagtttactg
    11941 ttgaacaact acagcagagt gaatacttgc agcttccggc atttatcacc gtaccgcctc
    12001 ccaccttatg gtttgtacaa cgacgccgat atttccgcat ctccgcccca ctccatccgc
    12061 cttatttttg ccagaccaaa ctggcggata acagtacgtt acgtttccgc ctgtatgatt
    12121 tgtcgttagg cggcatgggc gcattactgg aaacagcaaa gcctgccgaa ttacaagaag
    12181 gcatgcgctt cgctcagatt gaagtcaaca tggggcaatg gggtgttttt cactttgacg
    12241 cccagttaat ctccatcagc gagcgcaaag tgattgatgg caagaatgaa accatcacca
    12301 ctccccgtct gagcttccgt tttcttaacg tcagcccgac ggtggagcgg caattacagc
    12361 ggattatttt ctctctcgag cgagaagccc gggaaaaagc ggacaaagtg cgcgactgat
    12421 ttacatcgcg tccagtgcct gctcaagttt gtagatatag cgcggagcct gcggcgcagg
    12481 atgatttcgc gctacgtgat cgaggaactc gtcagcatcc aaatcgttga ttttgctgat
    12541 cgcctttttc cgatctgacg agaaagtccg tagcagcgca cctgccccgt tagcgtatga
    12601 caccaccagc gcatattgca gtaccttcgg atcttcaatg cctgccagcg ggccggtttc
    12661 cagaatattc aggtaagccg cccccattga aatattacgc tctggatttt tcagctcgct
    12721 ggtcgtcggc tcaccactcc agcccatacg gcgataaaca tcacgtccgg aggttgaagc
    12781 ttttaactgc atcaaaccaa tggcattcga tttactcacc gcattcggat taccacccga
    12841 ttcgatagcg ataatcgccg tgatcaattg tggatcgacg ccccaggctg caccggcttt
    12901 ctggcttatt ggcatccact gcatcgcacg ttgcaccgga actttcgcgt tccacggcgg
    12961 gttcgtatag tcatgctttg atgaacaacc cgctaataac acaatcaaaa aggcaaacca
    13021 tctcaatttc acttcttcta tccttatagc ctggaatcgc cgttgaggtg acaacggcac
    13081 acctaagcgg catgatatac atttcgtttc tgttgtcagc aaggaattgc catgtctctg
    13141 tttcacttaa ttgccccatc gggttactgc attaaacagc acgccgcgct gcgtggtatc
    13201 cagcgcctta ccgacgaggg gcatcaggtc aataacgtcg aggttattgc ccgtcgttgt
    13261 gagcgttttg cgggtacgga aacggagcgc ctggaagatc tcaattccct tgccagactg
    13321 accaccccca acaccatcgt gctggctgta cgcggcggtt acggtgccag tcgtttactg
    13381 gcagatattg actggcaggc actggttgct cgccaacaat atgacccgtt gctcatatgc
    13441 gggcatagcg attttaccgc cattcagtgc ggtcttctgg cgcaaggcaa tgtcatcacc
    13501 tttagcggcc ccatgctggt agcaaacttt ggcgcggatg aactgaacgc ctttaccgag
    13561 caccatttct ggctggcgtt acgcaatgaa accttcacca ttgaatggca aggggaaggc
    13621 ccgacatgtc agaccgaagg cacgttgtgg ggaggcaatc ttgcgatgct gatttcactg
    13681 attggtacac cgtggatgcc aaaaattgag aacggtattc tggtgctgga agatattaac
    13741 gagcaccctt tccgcgtcga acgtatgctg ttgcagctct atcatgcggg aattttgcca
    13801 cgtcagaagg cgattattct cggtagcttt agcggcagca cgcccaatga ttatgacgca
    13861 ggttacaacc tggagtcagt gtacgcgttt ttgcgttccc gcctgtcgat tccactcatt
    13921 accggcctcg attttggtca tgaacaacgc acggttacgc tgccgttggg cgcacatgcc
    13981 attctgaata acacccggga aggcactcag ttgacgatta gcggtcatcc tgttcttaaa
    14041 atgtaaaaaa atcacgcttc agggctaagt aaaacgctgt ctctgccgct aatatggtag
    14101 ggtttatata agagacagcg taatcaggag taaccgaccg ttggatgcca caacaataat
    14161 tagccttttc atcttaggat ccatccttgt caccagcagt atattactta gttcattttc
    14221 ttcccgtctt ggcattccta ttctggttat ttttctggcg atcggcatgc tggcaggagt
    14281 cgatggcgtc ggcggtatcc cgtttgataa ttaccccttc gcctacatgg taagtaacct
    14341 ggcactggcg attattttgc tcgacggcgg gatgcgcact caggccagtt cctttcgtgt
    14401 ggcgttagga ccggcactgt cgctggcgac gctgggcgtg cttatcacct ctggtttaac
    14461 cggcatgatg gcggcgtggc tgtttaatct tgatttgatt gaaggcttat taatcggcgc
    14521 tatcgtcggc tctaccgatg ctgcagcggt cttttctctg ctgggtggta aggggcttaa
    14581 cgaacgtgtt ggctcgacgc tggaaattga atccggcagt aatgatccaa tggcggtctt
    14641 tctgacgatt accctgattg cgatgatcca gcaacatgaa agcagtgtca gttggatgtt
    14701 cgtggtcgat attctgcagc aatttggcct cgggattgtc atcggacttg gtggtggtta
    14761 tttactgctg caaatgatta atcgcatcgc cctgcccgcc ggattatatc cattgctggc
    14821 attaagcggc ggtattttaa tttttgcttt aaccacggcg ctggaaggca gcggtattct
    14881 ggctgtttat ctgtgcggtt ttctgctggg taatcggccg cttcgcaacc gctacggcat
    14941 cctgcaaaat ttcgacggcc tcgcctggct ggcgcaaatc gccatgttcc tggtgctggg
    15001 gctgttggtt aacccaagcg atctgctgcc tattgccatt ccggcgctca ttttgtcggc
    15061 atggatgata ttcttcgccc gtcccctttc ggtatttgct ggattgctcc ccttccgcgg
    15121 tttcaatctg cgtgagcgcg tgtttatcag ctgggtagga ttacgcggcg cggtgccaat
    15181 catcctggct gtgttcccga tgatggcggg gctggagaat gcacgtctgt tctttaatgt
    15241 cgccttcttt gtggttctgg tttcactgct cttgcaggga acatcactct cgtgggcggc
    15301 taaaaaagcc aaagtggtgg ttccgccagt gggacgtccg gtgtcacgcg ttggcctgga
    15361 tattcatccg gaaaatccgt gggagcagtt tgtttatcaa ttgagtgccg ataaatggtg
    15421 cgtgggcgcg gcactgcgcg atttgcatat gccaaaagag acgcgtattg cggcactgtt
    15481 tcgtgataac cagttgcttc atcctaccgg cagcacccga ctgcgcgaag gcgatgtgtt
    15541 gtgtgtaatt ggtcgggaac gcgatcttcc ggcgctcggt aaactgttca gccagtcgcc
    15601 gccggtcgcg ctggatcaac gcttctttgg tgacttcatt ctcgaagcca gcgccaaata
    15661 tgctgatgtt gcgctgatat atggcctgga agacgggcga gaatatcgtg ataagcagca
    15721 aacgctgggt gaaattgtcc agcagttgtt aggcgcggca ccggttgtcg gtgaccaggt
    15781 agagtttgcc gggatgatct ggacggtggc cgagaaagaa gataatgaag tgttgaagat
    15841 tggcgtacgg gtggctgaag aggaagctga atcttaaatt taatgaagca tctatcgccg
    15901 gttgcgatgc tttgctgaac gcagcctgag cctgcaattg tttgtcgtcc ataatttaac
    15961 cttcatttga tgttgaattg aacatatcaa aatcaggcaa ttacacaaat ctatgtacag
    16021 gctccatcaa gggtaaagcg tgatttatct gaagtcgagt tcgagtcaac aatgaagcgt
    16081 aagcgtttta tgtagaacgc ctgtgcggtg agttcaggtt ccggggaaat agtgtccatt
    16141 cttttaatgg acactatcat gggcaatggc tctgtcacat taacaactac agttgctgac
    16201 cagccggatg gatgattttt tcgcaaccag aagacgctgc ctccgatccg gcttacaaca
    16261 agttacactg tcacaaccgg gacgcgtaac gccagcgcgc acatcaactc atagcccacc
    16321 gttccggcag cggcggcgac atcatcaatt ttgatctcct tgccccacag ctcaaccggc
    16381 gtaccaatcc ccgcctgcgg gcaaggcgtt aaatcgaccg ctagcatatc catcgagacg
    16441 gtccccaccg tcatggtgcg cacgccgtcc actaaaacag gggtaccggt aggcgcgtgg
    16501 cgcggataac cgtcggcgta ccctgcggcg acaatgccga ttcgctgttc atcgcgcgca
    16561 gtatagcgac cgccgtagcc cacacgctca cccgctttta gcgtctggac accaataatc
    16621 tcactgctta gcgtcatcac cggacgtaat ccggtattgg cgatatcacg ccactgaccg
    16681 gacggcgaag cgccatacaa aataatgcca ggccgaaccc agtcaaaatg cgcttccggg
    16741 tgccacagag tcgccgccga attggacaac gaacgccgac actccagccc ttccgccgcc
    16801 tgctcaatac gcgccatcgc gctggaaatt ccatcaggat gttccgcttc ggcaaaatgc
    16861 gacatcaggg tcatctcgcc aacattcgcc attgcccgca actgctgcca gacggtaagc
    16921 acgcgatcgg gctggaagcc caaccgattc atcccactgt tcactttaag ataaatatcc
    16981 aacggtgctt ttagccgcgc attttgcagt gctttgagct gccagttgct gtgtacgcag
    17041 gtggtcaggc ggtgctggtc ataaatctcc agatcctgag catggaaaaa tccttccagc
    17101 atcaggatcg gccccttcca gccgcgctca cgtaacgtta ttgcctcttc caggttaagt
    17161 aatgcaaagc catcggtggc cccgagcgcg ctccagatac gctcaatacc atgcccgtaa
    17221 gcgttcgctt ttaccaccga ccagacgcgc gcgtgcggcg cggcctggcg gacaatggac
    17281 agattctgtt ttaatgcctg cagatcgagg ctggcctgta tcggacgggt catctcgttt
    17341 ccttagctgt gtgcgccatg taaatggccc ggacgtgatg gcgtaaatcc acggctgtag
    17401 cgcgctacgc ttagatcctc atatgggatc gctggcgtgc gaccagagag cagatcgctt
    17461 aacaactgac cggaaccgca agccatcgtc cagccgagcg tgccgtgacc ggtattcagc
    17521 cacagatttt taaagcgtgt acgcccgaca accggcgtac cgtctggcgt catcgggcgc
    17581 aaacccgtcc agaaagtcgc ctgctcgaca tgaccgccgc gtggatagag atcgcgaacc
    17641 accatttcca gcgtttcacg acgcggttgc aacagctcgg tgttaaaacc aacaatttcc
    17701 gccattccgc caacacgaat gcggttatcg aaacgggtaa tggcgatttt gtaggtttca
    17761 tcaagaatag tggatactgg cgcaccatct tcctgcgcaa ttggaatagt cagcgagtag
    17821 cctttcagcg gatgcaccgg aatatcaaca atgcctttga gcatcgccgt cgagtaagaa
    17881 ccaaacgcca tcacatacgc atcggcctta atcacttcat cgccacactt cacgccgtag
    17941 atttgctcgc cgtcgcaaag cagttggtca acgggcgtat taaagcggaa tttaaccccc
    18001 gcctgctctg ccatccgcgc cagattctgg gtaaacagct ggcagtctcc ggtttcatca
    18061 ttgggcagtt gcagaccacc cgtcagtttg tgcgctactt ctgccagcgc gggctccact
    18121 tccgccaggc ggctggattc cagcagctga tacggtacgc cggcatcttc cagcacggcg
    18181 atatcgcggg tcgcattttc atactgttgt tcggtacgga acagttgcag cgtcccaccc
    18241 tgacgccctt catactgaat attggtttcg gcgcgcaatg ctttcaggca atcacggctg
    18301 tattccgcca gacgcaccat ccgccctttg ttttccatgt agtggctggt gtcgcagtta
    18361 cgtaacattt gccacatcca tttcaactga aactgcgtac cgtcgagacg gaccgccagc
    18421 ggcgcatggc gctggaacat ccatttgatc gcttttaaag gcacacctgg tgccgcccac
    18481 ggtgcagcat atccggggga gatttgcccg gcattcgcgg cgctggtttc cagggcggcc
    18541 cccggctccc gatcaatgac ggtgacctca tgtcctgcct gatttaagta ccaggcgctg
    18601 gcaacgccta ccacaccact tcccagtatg acaactcgca tagccactcc gataacggta
    18661 gatacagtaa aagaataata atctaattac atcttgataa ctcagttgaa aatattattc
    18721 aacatacggc ttttttatgg tgagctggct cacatctccc tgactctgcg gcaatatggc
    18781 gtctttagta tctcctgctg aacgcccttt ttatccagca taaaattccg ttcagaagcg
    18841 gattagtggc actctgataa taagaaatcg caaagaaaaa aattctccgg cgacgcggtt
    18901 tttaacaggt gttctatgct tgaaatgagg tgctccacac agagagcgcc gacaacaatg
    18961 aggtgcgcgt atggcgacga tcgattctat gaataaggac accacacgtt tgagcgatgg
    19021 acccgactgg acgttcgacc tgctggatgt ttatctggca gagatagacc gggtggcgaa
    19081 actctaccgg ctggatacct acccgcacca gattgaagtg ataacctcag aacagatgat
    19141 ggatgcctac tccagcgtcg gcatgccaat taactatccg cactggtcat tcggtaaaaa
    19201 gtttatcgaa actgaacggc tgtataagca cggtcagcaa ggactggcct atgaaatcgt
    19261 cattaactct aacccgtgta tcgcttacct gatggaagag aacaccatta ccatgcaggc
    19321 gctggtgatg gcacacgcct gctatgggca taactctttc tttaaaaaca attacttatt
    19381 ccgcagttgg accgacgcca gttcgattgt cgattatctg atttttgccc gtaaatatat
    19441 taccgagtgc gaagaacgtt atggcgttga tgaagtagaa cggcttctgg actcgtgcca
    19501 cgcgctgatg aactacggcg tggaccggta caaacgcccg caaaaaatct cactgcaaga
    19561 agagaaagcc cggcagaaaa gtcgcgaaga gtatctacaa agtcaggtca atatgctctg
    19621 gcgtaccctg ccgaagcgcg aggaagagaa aacggttgct gaagcgcgcc gctatccgtc
    19681 cgaaccacaa gaaaacctgc tctattttat ggagaaaaat gcgccactgc tggaatcatg
    19741 gcagcgtgaa atcctgcgta ttgtgcgtaa ggtgagccag tatttttatc cgcaaaaaca
    19801 gactcaggtg atgaacgaag gctgggcgac cttctggcac tataccatcc ttaaccatct
    19861 gtatgatgaa gggaaagtaa cggaacgttt tatgctggag tttttgcaca gccacaccaa
    19921 tgtggtcttc cagcccccct ataacagccc gtggtacagc ggcatcaacc cgtatgccct
    19981 cgggttcgcc atgttccagg atattaaacg gatttgtcag tcgccaacgg aagaagacaa
    20041 atactggttc ccggatatcg ccggttccga ctggctggaa acgctgcatt tcgcgatgcg
    20101 tgatttcaaa gatgagagtt ttatcagcca gtttctgtca ccgaaagtga tgcgtgattt
    20161 ccgcttcttc accgtgctgg atgatgatcg gcataattat ctggagattt ccgctattca
    20221 taatgaagaa ggttatcggg agatccgtaa ccggttatcg tcgcaatata acttaagtaa
    20281 tctggagccg aatattcaga tctggaacgt ggatttgcgc ggcgaccgtt cgctgacgct
    20341 gcgttacatt ccacataatc gcgcaccgct ggatcggggg cgcaaagaag tgctgaagca
    20401 tgtgcatcgc ctgtggggat ttgatgtgat gctcgaacag caaaacgaag acggcagcat
    20461 cgagttgctg gaacgttgcc cgccaagaat gggaaatctg taaaaacaac aaaaaacccc
    20521 tcgtttgagg ggtttgctct ttaaacggaa gggattatcg cccctgaatg gctaaatcac
    20581 ccggcagatt tttctgcatc cggtgccaaa tctcgccact ctcatgccca tagcgacgca
    20641 ctgtttcgta cacctgatcg tgcgcgcctt cactgcacaa cgccgacagt ttgtggtaga
    20701 agcccagcgc cagactgcgc gcttccggat tggcgaaata gtgacgacca atgcgcgtat
    20761 acagcccttt catcccgtta aggatcagac cgtaaatcgg gttgccggaa gcaaacgcca
    20821 ggccgcggaa tatgttgtaa tccagctcgg caaaggcatc ggcgtgatcg gccacttcat
    20881 tagcggtagc cagcacttcc tgcgctttat cgggatgctg acgaaacgcg gtgcgaataa
    20941 aaatagtgga aatattggta cgcaccgaca gcaaattatc aataagctgc ggcacacttt
    21001 cgtgatccag tcgcgccagt gtttcaagga tatttaaacc agaggtttcc cagaaattat
    21061 tcaccttcgt cggcttgcca tgttgaatgg tcaaccagcc atctcgtgcc agacgctgta
    21121 acacttcacg taacgtagta cgcgttacgc caattaattc tgaaagttca cgttctgcgg
    21181 gcaaaatagt cccgggaggg aagcggttat tccagatact ttcaataatg tactcttccg
    21241 cgaaacccgc cgggctttgc gccttaatga ccatagtgag atttccataa cacagcaaaa
    21301 caaagttgga ctcatcatac cagagggcta actacgctga tagcagacaa aaagaagaaa
    21361 aaagggatca aggcttcatt ttccgcatct ctttcccctt gactatcaag acttgcctca
    21421 ctcctgcctg accgttaagc tttgcgttta aacataaaaa catcattgat tatttgtaag
    21481 gtaagggaaa ccatcatgga gatctcctgg ggccgcgcgc tatggcgcaa ttttttgggc
    21541 cagtcccccg actggtacaa actcgccctc atcattttct taatcgtaaa cccgttaatt
    21601 ttcctcatca gccctttcgt cgcgggctgg ttgctggtag cggaatttat tttcactctg
    21661 gcgatggccc tgaaatgcta cccgctgctc cccggtggtc tgttggctat cgaagcggta
    21721 ttcatcggca tgaccagcgc ggaacacgtc cgtgaagagg tggcggcaaa tcttgaagtc
    21781 ttgctgttat tgatgtttat ggtggcgggt atctatttta tgaaacagct gttgctgttc
    21841 atatttaccc gtttgctgtt aagcattcgc tccaaaatgc tgctgtcgct ctctttttgc
    21901 gtggcggcgg cgttcctctc cgcgttcctc gatgccttaa ccgtcgtggc ggtggtgatc
    21961 agcgttgcag tcggttttta tggtatttat caccgcgtag cctcttcccg tactgaagat
    22021 accgacctgc aagacgatag tcatatcgac aagcattaca aagtggttct ggaacagttc
    22081 cgtggctttc tgcgtagcct gatgatgcat gccggtgtcg gcactgcatt aggcggcgtg
    22141 atgaccatgg tgggcgaacc acagaacctg atcatcgcta aagcggctgg ctggcatttt
    22201 ggcgatttct tcctgcgcat gtcgccggtg accgttccgg ttctgatttg tggcctgtta
    22261 acctgcctgc tggtagagaa gctgcgttgg tttggctacg gtgaaacgct gccggagaaa
    22321 gtccgcgaag tgttgcaaca gtttgacgat caaagccgcc accagcgtac tcgtcaggat
    22381 aaaatccgtc tgattgtcca ggcgattatt ggcgtctggc tggtgactgc gctggcgttg
    22441 catctggcgg aagttggctt gattggtttg tcagtcatta ttctggcgac atcattgacc
    22501 ggtgtcaccg atgaacatgc tatcggtaaa gccttcaccg aatctctgcc attcaccgca
    22561 ctgttgacgg tgtttttctc ggtagtcgcg gtgattatcg accaacaact gttttcgcca
    22621 attattcagt ttgtgttgca ggcatcggaa catgctcagc tgtcgctgtt ctatattttc
    22681 aacggtctgc tgtcatccat ttcggataac gtcttcgtgg ggacgattta tatcaacgaa
    22741 gcgaaagcgg caatggaaag tggcgctatc acgttgaagc aatacgagct gctggcggtc
    22801 gccattaata ccggtaccaa tctgccctcc gtcgctacgc cgaacggtca ggctgcgttc
    22861 ctgttcctgc tgacctctgc actcgcgcca ttgattcgcc tctcttatgg ccgcatggtg
    22921 tggatggccc tgccttacac cctcgtactg acactcgtcg gcttgctctg cgtcgagttt
    22981 acgcttgccc ctgtaaccga atggtttatg caaatgggct ggatagcaac gctttgataa
    23041 caacttaccg ggcatataaa tgcccggttt gccttttcgc ccgataattg tccaattgcg
    23101 tcctattttt gctgcctcct ggtggcgcag cgaatgaatt ggtttaaact gcgcactcta
    23161 tgcatattgc agggaaatga ttatgttgcg atttttgaac caatgttcac aaggccgggg
    23221 cgcgtggttg ttgatggcgt ttactgctct ggcactggaa ctgacggcgc tgtggttcca
    23281 gcatgtgatg ttactgaaac cttgcgtgct ctgtatttat gaacgctgcg cgttattcgg
    23341 cgttctgggt gctgcgctga ttggcgcgat cgccccgaaa actccgctgc gttatgtagc
    23401 gatggttatc tggttgtata gtgcgttccg cggtgtgcag ttaacttacg agcacaccat
    23461 gcttcagctc tatccttcgc cgtttgccac ctgtgatttt atggttcgtt tcccggaatg
    23521 gctgccgctg gataaatggg tgccgcaagt gtttgtcgcc tctggcgatt gcgccgagcg
    23581 tcagtgggat tttttaggta tggaaatgcc gcagtggctg ctcggtattt ttatcgctta
    23641 cctgattgtc gcggtgctgg tggtgatttc ccagccgttt aaagcgaaaa aacgtgacct
    23701 gttcggtcgc taatccattc ggcgctcctg cgggagcgct tttttcctgc cgctatattt
    23761 atttgacccg cagtaaatca gaacttcgcg ttgtataacg tggtgaaagc atggcacgct
    23821 tcatctgcca ttgttgctgg atcccctgcc cggcaaaata gagtgttcct ctgccctctt
    23881 tagcattcag tgtatccatt accgtcatca attgctcact cccggggcgc ggtgcgttgt
    23941 catcgaataa attgagctgc gcgactccct gactgaagaa atcccccagc atcacgcccg
    24001 ctttttggta gcgatggccc gcttgccaga tggcatccag agatcgcgta gcagcgttaa
    24061 tgatatccct gctgtcctga gtgggcgtca gcagttttac cgacgcgcta ttgccgtaat
    24121 aaggttcatt gagcgcaaat ggtgacgtct taataaatgt ggagataaac cgacaatatt
    24181 gatgctcgct gcgaagtttt tccgccgccc gggcagcgta actacaaatg gcctgccgca
    24241 tcgatggata atccgtgatg cgttcaccaa acgagcggga acagataatt tcctgcttcg
    24301 tcggtgcaaa ctcttccagt tgcaaacagg gttcgccgcg cagttcacgc accgttcttt
    24361 cgagcacgac attaaaatgt ttacggataa accggatatc tgtatccgcc aaatcaagaa
    24421 cggttttgat ccccatcgcg tccagttttt tgctgatccg ccgtccaatc ccccagactt
    24481 catccacggg gagagcagac attaatttac gctggcgttc cagatttgat aaatccacca
    24541 ccccccccgt ctgccgctgc catttttttg ccgcatgatt ggcaagctta gccagcgttt
    24601 tggtctgggc gatccccaca ccaacagtaa gatgggtacg ttgtagcacc gttgcgcgaa
    24661 tttctctgcc aaaatcagtc agatcgcgac aattacgcac acctgtcaga tcgcagaatg
    24721 cctcatcaat actgtaaatc tcgacgcggg gcgatagttc ttccagcgtc gacatcaccc
    24781 gattgctcat gtctgcgtaa agctcataat tgctgctaaa gcaaaccaca ccacagcgac
    24841 gaaacagatc tttttgtttg aaccagggat cgcccatttt aacacccagc gcctttgcct
    24901 cagcgtttcg ggcgataacg caaccgtcat tattcgatag cacaaccacc ggtttacccc
    24961 ataaatcagg gcgaaacacc gtctcacagc tggcataaaa cgcgtttaca tcacagagag
    25021 caaacatcag cgcatcgcct taacgacgtg gatcaccaca ccaaagacat ccagcgtatc
    25081 ttcgctactg atggtaatgg gcgagtacgc actgttcatg ggaataagct gtaccgtcgg
    25141 gcgtagttgc aattttttca ccgtaaactc gccgtcaaca gcagcgatga caatatcacc
    25201 atggctggcg gtaatagcgc tatcgacaat cagtaaatca ccgtcactaa ttccaccatc
    25261 aatcatagaa tcaccacttg ctttgacgaa gtaagtcgcg ctgggatgct ggatcaacag
    25321 ttgattcaga tcgatgcgct gttcaacgta atctgctgcc ggtgaaggaa agccacactg
    25381 aacaagatcg ctaaatagcg gaaaagtcac aatttcgcgg agatccgcag gcttgataaa
    25441 caacataata atctgcctga agctatactg tttttatata cagtagtctg ttcttgccag
    25501 cagatcaata ctgattcagg ctatcaatat ttgtcgctgc atagactgct gatttttcgt
    25561 tctcttatcc tgtgctcacg cggccttctg gcgactacgc tcatccagca gaaatgaaaa
    25621 atattacccg gctaaaaaat agaatagaag cattcgccat aatgacatta aacattgttt
    25681 gatatttatc atattaatag aaataaagac attgacgcat cccgcccggc taactatgaa
    25741 ttagatgaag taaaatttat taatagttgt aaaacaggag tttcattaca atttatatat
    25801 ttaaagaggc gaatgattat gactgaaatc gttgcagata aaacggtaga agtagttaaa
    25861 aacgcaatcg aaaccgcaga tggagcatta gatctttata ataaatatct cgatcaggtc
    25921 atcccctggc agacctttga tgaaaccata aaagagttaa gtcgctttaa acaggagtat
    25981 tcacaggcag cctccgtttt agtcggcgat attaaaacct tacttatgga tagccaggat
    26041 aagtattttg aagcaaccca aacagtgtat gaatggtgtg gtgttgcgac gcaattgctc
    26101 gcagcgtata ttttgctatt tgatgagtac aatgagaaga aagcatccgc ccagaaagac
    26161 attctcatta aggtactgga tgacggcatc acgaagctga atgaagcgca aaaatccctg
    26221 ctggtaagct cacaaagttt caacaacgct tccgggaaac tgctggcgtt agatagccag
    26281 ttaaccaatg atttttcaga aaaaagcagc tatttccagt cacaggtaga taaaatcagg
    26341 aaggaagcgt atgccggtgc cgcagccggt gtcgtcgccg gtccatttgg attaatcatt
    26401 tcctattcta ttgctgcggg cgtagttgaa gggaaactga ttccagaatt gaagaacaag
    26461 ttaaaatctg tgcagaattt ctttaccacc ctgtctaaca cggttaaaca agcgaataaa
    26521 gatatcgatg ccgccaaatt gaaattaacc accgaaatag ccgccatcgg ggagataaaa
    26581 acggaaactg aaacaaccag attctatgtt gattatgatg atttaatgct ttctttgcta
    26641 aaagaagcgg caaaaaaaat gattaacacc tgtaatgagt atcagaaaag acacggtaaa
    26701 aagacactct ttgaggtacc tgaagtctga taagcgatta ttctctccat gtactcaagg
    26761 tataaggttt atcacattta aaaacacaga cgttttttaa agattgagag ttacctgttg
    26821 ataataaggt atcgggtaac tctcattatg acagagcgct ttcaaaaaca gggctacgaa
    26881 atggcgagca tccagacgta tattaagaaa gcccccatca acatctatta aatttatcac
    26941 tgcgcccagt caggtttatt taagatatga tcctgccagt caatcacttc tgattctttc
    27001 actgcgatat gacgcacaga gatacgttca ccatgcattg ccgctttcga acccataagt
    27061 agcggatgcc acgcaggtaa atctttacct tccgccagca accgataagc gcaggtcatt
    27121 ggcagccatt cgaatgttgg cagattttca cgggttaatt taatgcagtc gggttcaaac
    27181 tcgaaacgac gttcgtagtt ccgacattga caggttttaa tattgagctg gcgacaggcg
    27241 acgttagtga agtagatttc gtcggtgtct tcatccatca gtttatgcag gcaacactga
    27301 ccgcaaccat cacacaacga ctcccattcc gcatcgctca tttcgtccag ggttttactt
    27361 tgccagaaag gtacatcgct catcaggtgt tccgccatta cgttaaaacg caccttataa
    27421 ccagtctggc acagcgatgc aagttttgcc gccgctttca ggcggcaaaa agtattacaa
    27481 aacgcgagtt gccaaagaat gcccatcgaa agtgactgtc agctcatcac cgctttgcaa
    27541 cgggccgacg ccatcaggcg tgcctgtcag cacaacgtca ccggccttga gggtaaaaaa
    27601 cttgctcata taagcgatca gcggaacaat tttatggatc atgtccgcag tcgtaccttg
    27661 ctgacgttgc tcgccgttta cgctcaggcc cagcgttgta ttttgcgggt cgccggtaaa
    27721 ttccgccgcg ggaataaacc cggaaagcgg acaagagtta tcaaacgctt tagccttttc
    27781 ccacggctgc ccggctttct tcatttttcc ctgaacatca cgcaatgtca gatcgagcgc
    27841 cacgccataa ccggcaatgg ctttgcggac atgctcttcc gtagcctgac gcagcgtcgc
    27901 gccaatcaac accgccagtt cgacttcatg atgaactgaa ccgaaatcgg atgggatcgc
    27961 caatggctgc cgcagatcgc acagtgccgt ttctggttta ataaacagca ctggctcttc
    28021 gggcactgcg ctgcccatct ctttaatatg tttggcatag ttactgccaa cacagactac
    28081 tttactcacc ggataatcca gcagcgcacc ttgccagttg tgatgttgat acataacgtt
    28141 cccctcacct tatcgtggat atgaaaaccc gcgccgacaa agataatgcc accgccggat
    28201 atcggttatt tgttagtgtc gtctgttttc tgccccatga cggaaagatg ttgcttcagc
    28261 aaatcttcgg gtggtggcgg taactgcaaa tagtaacctt gctcggttaa tgccagttta
    28321 accttttcaa tatcagcatt gaccagtttc ttccgcccat ccaacggcag aatcatcgct
    28381 aactgaggct gaccaaaacc tttcatcagt tcctcaggaa cacgcgaaaa atcgtctttt
    28441 ttttcgacat ataaataggt ctggtcacgc ttgctgcttc gataaatcac acaaaacata
    28501 cttttactct ttaagatgcc aggttttggc ataagctaac atcaatgaaa cccgcgccac
    28561 aagccagcca gctgtagatt tctacggttt gttgagtcca tgccccttta taagtaatcc
    28621 ccccggcaaa aatgccgcgc cgggatttta ccgaacgcag gaaagccact ggaattctcc
    28681 cgttagggat tgttatatta taacagttca tcgtactcat tctgaacagg agactaccaa
    28741 tgaaaatcaa gagcatcagt aaggctgtat tgctgtttgc actgctaacc tccacgagct
    28801 ttgctgcggg taagaatgtc aatgttgagt tcagaaaagg ccatagcagc gctcagtact
    28861 ccggcgaaat aaagggatac gattacgata catatacctt ctatgccaaa aaaggtcaga
    28921 aagtgcatgt gagtatctct aatgaaggcg ctgatactta cctcttcggg ccaggaatcg
    28981 atgattcagt tgatttatcc agatattccc cagaactcga tagtcacgga caatactcac
    29041 tccctgcttc tggcaaatat gagctgcgag tgttgcaaac ccgaaacgac gcccgtaaaa
    29101 acaaaactaa aaaatataac gtcgatattc aaattaagta aatgtcaacc gacggggact
    29161 ctccccgtca taaacgttaa cgccccactc ctctctgtcg ctcaagaggt taatgacgaa
    29221 tagaacataa tgtaaacaag acatcactca cgacacataa ataacatctt tgtggtggtt
    29281 tgattgattt ttccactccg cttaccatct acaccaaaat aacgatcatc acgacataat
    29341 ttttctttaa catcgcaaaa tacaccgtta gcgaatgtaa attcgctggg atcgtagcct
    29401 tgtaatgatt gtaagttttc agcggctttt tcgccgagat atttccttgt taactctggg
    29461 gaaatgccat ctgagttcac acaaatatac gggtcgcaaa caacgccgca cgccggagat
    29521 tgaagtccgc tagcgtcatg tccagacgcc attgatgaga acatcatccc cacacctgac
    29581 aatccgatat aaataatacg catcatattc atagcacatc ctgataatcc aaccgtacaa
    29641 cacttatgct catttcgtca tcaatccgta atcaaaaccg ctgacctgga cgagttacga
    29701 tgacctgaca gaagtatcag acgtcttctg cggtttctat gatagagcaa ttctgtggcg
    29761 gatattaaga taacgggtat ctgttcttta atgatgccgc aaaaaatgga aagttaagaa
    29821 agcctggcgt ggtgaaaaaa tacacttgcc tcaatactgg atatttaaac aggtctggag
    29881 atgctgtcac gattagggtg cggaaaaagg aagcgtaaat aagaagaatc aaaagtacga
    29941 cggcaatggg ttgattgaca agggtatttt ttaagctatg aatcagcgcc atttatcaca
    30001 gaatagactt ttactctgaa taaatgggag ggtgacttgc ctcaatataa tccagactat
    30061 aacatgcctt atagtcttcg gaacatcatc gcgcgctggc gatgattaat agctaattga
    30121 gtaaggccag gatgtcaaac acgccaatcg agcttaaagg cagtagcttc actttatctg
    30181 tggttcatct gcatgaggca gaacctaagg ttatccatca ggcgctggaa gacaaaatcg
    30241 ctcaggcccc cgcattttta aaacatgccc ccgttgtact caacgtcagt gcactggaag
    30301 acccggtaaa ctggtcagcg atgcataagg cggtttcggc aaccggtttg cgggttattg
    30361 gcgtaagtgg ctgcaaagat gcgcaactta aagccgaaat tgaaaaaatg gggctgccta
    30421 tcctgacgga aggaaaggaa aaagcgccac gtccagctcc cgcaccgcag gctccagcgc
    30481 aaaatacaac gccggtcaca aaaacgcgtt taatagatac cccggtgcgt tccggtcagc
    30541 gtatttatgc tccacaatgt gatctgattg ttacaagcca cgttagcgct ggggccgaat
    30601 tgattgccga tggtaacatt catgtctatg gcatgatgcg cggtcgtgcg ctggcagggg
    30661 caagtggtga ccgggaaacg caaatatttt gtacgaacct gatggcggaa ctggtgtcca
    30721 tcgcaggtga atactggctg agtgatcaaa tcccagcaga attttatggc aaagcggcgc
    30781 gactgcagtt agtcgaaaac gctttgaccg ttcaaccgtt aaattgatcc ctttttaaca
    30841 aggaatttct atggcacgca ttattgttgt tacttcgggc aaagggggtg ttggtaagac
    30901 aacctccagc gcggccatcg ccactggttt ggcccagaag ggaaagaaaa ctgtcgtgat
    30961 agattttgat atcggcctgc gtaatctcga cctgattatg ggttgtgaac gccgggtcgt
    31021 ttacgatttc gtcaacgtca ttcagggcga tgcaacgcta aatcaggcgt taattaaaga
    31081 taagcgtact gaaaatctct atattcttcc ggcatcgcaa acacgcgata aagatgccct
    31141 cacccgtgaa ggggtcgcca aagttcttga tgatctgaaa gcgatggatt ttgaatttat
    31201 cgtttgtgac tccccggcag ggattgaaac cggtgcgtta atggcactct attttgcaga
    31261 cgaagccatt attaccacca acccggaagt ctcctcagta cgcgactctg accgtatttt
    31321 aggcattctg gcgtcgaaat cacgccgcgc agaaaatggc gaagagccta ttaaagagca
    31381 cctgctgtta acgcgctata acccaggccg cgtaagcaga ggtgacatgc tgagcatgga
    31441 agatgtgctg gagatcctgc gcatcaaact cgtcggcgtg atcccagagg atcaatcagt
    31501 attgcgcgcc tctaaccagg gtgaaccggt cattctcgac attaacgccg atgcgggtaa
    31561 agcctacgca gataccgtag aacgtctgtt gggagaagaa cgtcctttcc gcttcattga
    31621 agaagagaag aaaggcttcc tcaaacgctt gttcggagga taagttatgg cattactcga
    31681 tttctttctc tcgcggaaga aaaacacagc caacattgca aaagaacggc tgcagattat
    31741 tgttgctgaa cgccgtcgca gcgatgcaga accgcattat ctgccgcagt tgcgtaaaga
    31801 tattcttgag gtcatttgta aatacgtaca aattgatcct gagatggtaa ccgtacagct
    31861 tgagcaaaaa gatggcgata tttctattct tgagctgaac gtgaccttac cggaagcaga
    31921 agagctgaaa taagcccgct gtaaaaacgc atttatcttc aaggcagagt catttctgcc
    31981 ttgagttttt catccctctc atccacgttg tggtaaagcg gcgagtattc ttgctgatac
    32041 tcctcattgc tatttccacc tcccccttta caccttaagg ctgttcccac gcacagaaca
    32101 aaatgtttat gcctggttga gtaaataacc tcattgttag tgtggataca ctgggggatg
    32161 ataaccccgc aatttcatta ttcattgttc tcccatagga tgaggtttcc cgccgtaact
    32221 ggcgggcttt ttttgcccca gaattttccc ttttagcccc ctgtaaacga aaactgcgcc
    32281 gaagcgcagt ttaatcgttg atggtcagaa cgaccagttc acaccagcca ccgcattcca
    32341 cggggattcc acaccggcac catggctata ccccaccccg aaatgcccgc ttaacgtact
    32401 gctgaatgag gctttaatac ctgcctggta tattccacgt ctgcccgaca aatcattgac
    32461 aaaattaccg tcactattca ctttcacccg attatcatcg acaaattctt tgctcacagc
    32521 cgccttcagg cagggttcaa tttccatacc gttccccaga cgcatgttgt aactcagcgt
    32581 tgcacccagt tcacgatata tactgcgggt atcgactgat ttcgattcca tgccattgga
    32641 taaatgatag tagcgtctaa gtgacctaag ctctgctgca acatcagccc gcaggcacca
    32701 gaccagggga ttcatcctga agagacagcg caggtgtatt gtgttcaccg ctcaccaaag
    32761 acatcatgat gaaatgatga tatcccgcat aagaaagagg cattttttac acgcagtgcg
    32821 ctgaagtgta gttggataaa aaatcactct tttcagataa tactgtcgta cgcttatctt
    32881 tcgacaatga ggccgtcggc aaaataaaat gactaatgta atcgttgctg atgaatatct
    32941 tctgctcaca taaaaatcac acaataatat tgataaatca gattatctta cctttacaac
    33001 attgcgttaa cagtcaatga cacttcttga ataaaaatat tgtcaatcaa tgagttattc
    33061 ctcatttaag attattctca ttcacgtaaa aaacataatt cctatcatca cagccacgct
    33121 cattttgaat tatctgaata atagcgagcg ttctttagtt aagcagatct taattgaaga
    33181 cgaaattatt gtgtgtgcaa cttacttaat accggacatc taagttgaag aaaaaaatac
    33241 ttgccttcgg gcttatctca gcattgttct gctccacgcc agcgatggcg gatatgaatc
    33301 gcaccacgaa aggtgcattg ctaggcgcag gcattggttt acttactggc aatggtgtta
    33361 atggcgtact gaaaggtgca gctgttggcg ctggtgttgg tgcagtaaca gaaaaaggcc
    33421 gcgacggtaa aaatgcgcgt aaaggtgcaa aagtaggtgc cgccgttggt gctgtcactg
    33481 gcgttctgac cggcaatggc ctcgaagggg cgattaaagg tgccgtgatt ggcggtacgg
    33541 gtggtgcaat tctgggtaaa atgaaataag gaatgatgat gaaaaaattt gctttactgg
    33601 cgggcctgtt tgttttcgca ccaatgacct gggcgcagga ctacaatatt aaaaacggtt
    33661 tgccgtcaga aacctatatt acttgcgcag aagccaacga aatggcaaaa actgacagcg
    33721 cacaggttgc agaaattgtt gcggtaatgg gtaatgccag cgttgccagc cgtgatttaa
    33781 aaattgagca atctccggaa ctgagcgcca aagtggtaga aaaactgaat caggtttgcg
    33841 ctaaagaccc gcaaatgctg ttgatcacgg caattgatga caccatgcgt gctatcggca
    33901 aaaaataata tttcatatgt accgcgtcga tgaatacgac gcggtttttt agctaacggc
    33961 gggatcagaa cttatatttc aggcccacca tcactgcggt gtcattgtag ccattatcac
    34021 ccagctgcac gcccacattc ccccagacgc tggccgctgg attcaggttg ccatttacgc
    34081 caagtttaat ttcccccaaa ttactcgccc catcttgttc gactttcaca ccgttcattg
    34141 agacagcaaa atctttactg ttatggatcc agttcatttc aacaaaaggt tcaaagtcgt
    34201 caccgtgcgg accgctgttt ttctcctgag tacgaataaa ggtgcggaaa ccaagtcggg
    34261 tctggatgtt gttatttgcg tcattctcaa tacgtgagcc attggcttcg gtgtgcgcat
    34321 tatgatcgac gcccatccag atagcctgtg cctgtggctg gaatatccac gtgtatttcg
    34381 ccgcttccag accaaaggat ttattcaggg caaagccgta acccgcttcc agcgagacgg
    34441 ttgcgccttt cgcactatag gattcatagg ctaattcatc ccctttcacc gagtttttaa
    34501 accagctata ttgcgcccag gcgtcaatgt atgcgccttt tttactgatg tcatcggcaa
    34561 accaagtggc atacagccct gcgcaatagc ctctgacact ccctttcgaa cgataatccg
    34621 acacgctgga atgagttgag ttgtagtcgc gggcataacc agccatcact cccaaacgcc
    34681 aactgtcact gtcggtaaaa ccaccggtta acagctcggc ccctaactgc gagacgtagc
    34741 gatgcgaggt cgttctcaac tgtccgttgc tgtcacgcca ggcattatgc ccgccaattt
    34801 gacgtagcca aagtcggctt gaacgctcct gttcagttac aggatcgatg tagcgcattt
    34861 cacccgcacg gtcgttcaga cgcataacaa aaagagtgtt agcggctgcc aggttggcga
    34921 cgtaacttcc agcttcgagg cgcaggacgg gggtaggcgc aacagtaggc gctgaacttc
    34981 ctccggagtt tgtttcaccg ccgctcgttg cctggaagtt agtcagatac cagtttttat
    35041 tggtcgcttc ggtattacct cgggttaatg aatattcgta ggcaccggcg aaaatcctcg
    35101 aatccttaat aaattcccca tttgattccc cctcaacgct gataatctct atcccattta
    35161 acgtgtaagc tccgctgcct cctgcattgg taactttaac tcgggttgtt ccagaggtat
    35221 ttccattaat aacgacttta tccgtagctg aattatcatc acccaattca gttctgagat
    35281 gcaatacgcc attattacca acgtaatttt cagttattgt taatcttgtt ggttcgacat
    35341 cccgtccatc tgcccgggaa atatagaccg tactattatc aaccgtgagg ttgctcaccg
    35401 ttgagtcagc tttaatatcc caggtactat tatctgaaag cgacagatag gtatgagtgt
    35461 taacatctgt tgaaatatta gctgaacccg ttatttttga attagttgca tcgacggtca
    35521 aatgaccttt aatattattg ataaaaacat ctgcagccga tgatacctca ctatccgtaa
    35581 tggtcacgtt actcgtctca tttttatggt agaggatttc atttgctgac cctagtgagc
    35641 taccagataa aatatctacc gttgcttcct gaccatttat ggaagactcc agcacaattc
    35701 cagacccctg atgcgcgact gcagatgtat ttttcagcgt caccgtgccg ccattataaa
    35761 ttttagcagt cgcggcgtca ggacccgttg tataaatttc tccctggtta aatgaaactg
    35821 tcccctggcg ggcagcgatg gcatgagcat tgtccccatg cgtggtgata tcaacatcgg
    35881 ttaatgtaac gctggcattg gtataatcaa gcgtgtcacc agccttgacg acagtgccat
    35941 tagcgtacgc ggcataactt tctgttccgt gggttgtgat ctcccctttt gccatatcca
    36001 gttttgctat ggcattattt tcattacttc ctgtatcggt agtactttct tcatcaagag
    36061 aagatacaca cgcacgcgcg cttccgggag atgcacacgc aagaacgccg actgcatgat
    36121 tacccgcagt aacaataaat gtatcatctc cgtcagaaaa agagggggta accgaaccgt
    36181 cagagttgac tcttattatg gtgtcagtat tcaggtttcc actcgtttga cttagcaaac
    36241 caattgagta atcccccgtt atgacaatcg tgtttttacc ctgcaaaata acagcaccgc
    36301 caaattcact tacgattgcg ctagctgtta atgctgtgct tagaggacta ttgtcagaag
    36361 gatataaacc gcccgttgtt acaatgacgc cattgttaat ggttaacttg ctaccctctc
    36421 ctgcaacgga tacccgtaac ttccattacc cgttgttata actagcgcat tatcaatatc
    36481 ttttactttt ccatctttta ttgccacagc atgtttatat tcatcgtgct gaataaactg
    36541 ttcagcatat gcattaccca ccgccagagc aataagtccc aaactaaacc cggagagttt
    36601 tttcagtttc atagattaac catccattca ttggattaac aaacattcct ggtcaaataa
    36661 cctatgcgtt cagattacca aaacggaggg taaagacaaa aacaaaagca caaaaaattt
    36721 aaatagataa acagcgacaa tatataaatg ataaataagt cggcattatc atttttcacc
    36781 ttattttcat gacattgatc actttgaggt gacgattttg tttaacaaat aactcttcga
    36841 catcacaatc attgatagtt gcaataaaaa ttatttcaat gacatataaa aacaactgtg
    36901 ataacaccag ggttgacaac tatttgcgca ataattcctt tacttcaatg atccaggcat
    36961 tcactgccag caacccattc agccgcatac tgacagtgag aaactgaaag attattgctg
    37021 atccctgtgg ctgacgagtc tttcccttaa tcgattaacc agattgatcg ccagccaaac
    37081 aagggcatta agcaaattta tcgtcacaag cacttctccc agcccggtac tgggttttgc
    37141 aataaggatg accaggaggg tgatcagcat aaatgcaatg gaaaagatga tatagagagt
    37201 gaaataatcc agattattac tattgttcat gctactaatg gcaatcctgt caaaactttc
    37261 aaacaattat agcaaacaaa ataatgaaca atagtaacaa gaggcaacga gctaattagc
    37321 tgtctataat tacaccgcac tatggcctta ctgatcaatt ttcactcaac cacaaccttc
    37381 accttcggtt tagaaaggat aattttgacc aggtcgatgt atgtaacagg tttatagaaa
    37441 taataaccct gctgaaatgt gatattattt tggttcagat agtcaagttg ttcattcgtt
    37501 tcgacacctt cagcgacgat actgatcgaa agtttacgcg ccagatccaa tacacaatcc
    37561 agaattcggg ttgattctgg gtctgcgttt acgcggccaa cgaaactgtg atcaattttg
    37621 atataatcaa tatgcaggtc atgaagataa gaaagccctg agtagccagt accgaaatca
    37681 tccagcgcga tgacaaaacc attttcatgc agaatgttca accgctgaac cagactttca
    37741 tcaacattca atggctcacg ctcggtgact tccagaacaa ggtttaaatc gcggcgggta
    37801 aaactgtcac ggaaatttaa acactcgtcg acaaacgtcg gcgaaataat atgcgaagcg
    37861 ctaaaattaa ttccaatatg aaaaccttcc ggcaatttac tcgcgatagc gttcatctgt
    37921 ctggcaacct gattcatcag gctttgcgta agcggaacga ttaatccgga tttttcagca
    37981 agtggaataa atgccgcggg tgatatatat ccaccgtgag gttgtttcca gcgggctaac
    38041 acctcaactc cccgcaatgt cccttcccga ccatttacca caggttggta aaaagggacg
    38101 atctccccat tatttattgc ccgtcgcagg atctcttcag gcgttgcgct tttattgaaa
    38161 tatctatcaa gcaggaaggc agcggcgcag gcgattaaca gaataaatat taaaatccca
    38221 aagcctttat cgataagtct gtagaagcta aagagtggcg gcgggttaaa gataatagtg
    38281 aaatgatatt ttgtggagtg aaccttctcg gtatagggat aatgtccatt aagcgtcatc
    38341 acatcacccg ttggtccaat aatgtcatcc gccacgcgta atacatacct tacccctttc
    38401 aagggtacat taagtgcccc acgaatatgc tgatcgctta tcgtaaccaa aatgcgcgta
    38461 tctgcaaatt ggttctgata gagcaggata ggtaatctat taacggtgtc gattgctgga
    38521 gccaacagta aattactgtc cgggaaaaca ggaatacgag acaacaggac ccgattccca
    38581 ggcagggatg tacaccagac tattccctgt ttgagaatga tgattgtgcg caggtgaggt
    38641 ttcagagctg cttcagtgcc aagctgatat tgctcctcga ggttgcattc cttcccggca
    38701 atgttaaccg ctgtttgagt cgcttgtgac gcttcttcaa gtatatgatt gatattgttg
    38761 gcggcatatc tcgctcccgc caggtactct gcttttgcgg aataccagag ctggatgttc
    38821 aaaatagcga caccggtaat aaacaagcat atcgcaacga ggatgggtat gagtgtattg
    38881 cgcatttgct tgtcccggcc aaagggttta atacatgaaa atgccattca ttcgctcgcg
    38941 ctggcatcta cagagagcat ggtagagagc ataagaaata tccacgccga agataaaatt
    39001 ttctccatct tgatctaacc cattaattat tgagcataat gtaggcagta caaaataagt
    39061 taggcgggat atcaggcgtc aagaatggag agcgagaact ctccattctt gacacctgat
    39121 attgcggaca taataagaaa gcataacgcc tgaaatgctc actttgcatc agcatggtga
    39181 tacagctgat gtttattcta aaaccttact caagttctaa gagagcacgg attccctgtc
    39241 attaataatg atatccgtta gtgtagtctt atgcatttca atgtactgca atatttgcag
    39301 atagagatca gaaagaatca cactggtttc atcttttaaa cgcaagcgta aagcctgatc
    39361 aataatatga ctatcaacat acccatgcgt catgaacacc tccctctcga gatttttaaa
    39421 caaagcacat tgaaaaataa atctctctgg gattgaatta ttcatgtcag cctcgtagaa
    39481 atattaaccc aataaaatta tgctgtgtat agtctaaagc gacagacaaa aataagatga
    39541 atgagttttc tataaactta tacttaataa ttagaagtta catatcatca gctgtgtatc
    39601 gcaacacgat ttccagtgtt tttctaatca catcagcttt gagaatatca cttgttgttt
    39661 ccagggagtg tatcagactt aagataatat ttttattatt aacattatcg cctgaaagca
    39721 ttaaattggt gacagcctgc cctaacactg ctgactcttc ttctaacaaa ttacccgaac
    39781 tgcgaaagta acttgctaac gctttatcag ttattgcatt atgaattgta gtatcttcaa
    39841 gcatggtaac ctctcatctt acttatgaaa ttttaatgta ttctgtttat tttcttacca
    39901 ttgaacatac ttttattgtc agctgtcgct gttcttcggt ttggccctcc tgaatcacta
    39961 ttgctaaaat cacgaattat gctgtcaatt aaatcgcgtc ggaaagcatc ttcctcagcc
    40021 gatttcatta cgagtaattg cttaataaga gtctgcaatg aaaaatttat cttcatgcgc
    40081 agtatctgga tcaccgcctg gccagtaatt tcatatttta tacgttcatt atcagatgtc
    40141 ttcataacga cttcatagct aacctgttaa ataatcaggc tgttgcatta ttcaaaaagc
    40201 aacccaatta gtgcattata atgtttctgt tcctcttccc cagtcgcgtg ttccagtcgg
    40261 caaagaagtt tggcacaaag cgactttcga ctcagattac gtccatcttt caaaatctca
    40321 gtcacaattt ccccgagagt ttcctgctga gtaggcaagt tagcttttgc aaagtaacga
    40381 gtgattgctc ccgcagaatc taaagtcact gaattttgat gcatgctacg cctctgttaa
    40441 aaatgttaat caatgttaag tgaggtaaca atagcaaact tgtacgaaat atgtgtacag
    40501 aatgataaat taattttttg caattagtgc taatgcatat aaaacaatgt gttatgataa
    40561 aacatattgc atcatttaac tgacaatcta tacaagattt ttcgccaact agtgttcaac
    40621 tttttccatt ttttatcaat gtaaagaaac ggttatgttt tggtattaaa agaaagacac
    40681 aagaagatat gtctgttacc gtcttactct cgcctcaccc attaccctgg attgttaatg
    40741 cttaccaccc ttatttatcg tagccatata cgtgacgacg aacctgtcaa aaaaatcgaa
    40801 gaaatggttt cgatagcaaa tcgcaggaac atgcagtctg acgtaacagg gatcttactg
    40861 tttaatggtt ctcatttttt ccagcttctg gaaggtccgg aagaacaggt taaaatgata
    40921 tatcgggcta tatgccagga tccacggcac tataatattg ttgagctgct gtgcgattac
    40981 gcgcctgctc gccgttttgg caaagcggga atggaattat ttgatttgcg cctgcacgag
    41041 cgagatgacg ttttacaggc cgtattcgac aaaggcacat caaaatttca gctaacttat
    41101 gatgacagag cgctacaatt ttttcgtact tttgtccttg caaccgaaca atcaacctat
    41161 ttcgagatcc ctgccgaaga ctcctggctt tttatcgctg acggatctga taaagaactt
    41221 gattcctgtg ccctttcacc aactataaac gaccactttg cctttcatcc tattgtcgat
    41281 cccttatcgc ggcggataat cgcttttgaa gccattgtgc aaaaaaatga agatagccca
    41341 tcagccatag cggttgggca gcgtaaagac ggggaaatct acacagcgga tctcaaaagt
    41401 aaggcgcttg cattcacgat ggcacacgca cttgagctcg gtgataaaat gatttcaatc
    41461 aatctattac ctatgaccct ggttaacgaa cctgacgcag tctctttttt acttaatgaa
    41521 ataaaggcca atgctctggt gcctgaacaa atcatcgttg aatttactga aagtgaagtc
    41581 atatctcggt ttgatgagtt tgccgaagcg attaaatcgc taaaggctgc tggtatcagt
    41641 gtagcaattg atcattttgg cgcaggtttt gctggtttgt tactcctgtc acgcttccag
    41701 cctgacagaa ttaaaatcag tcaggaattg attaccaatg ttcataaaag tgggccacgg
    41761 caggcaatta ttcaggcgat cataaaatgc tgtacatcac ttgaaattca agtcagtgct
    41821 atgggcgtgg caacaccaga agagtggatg tggcttgaat ccgcaggaat tgagatgttt
    41881 cagggagatc tgtttgctaa agctaaattg aatggtatcc cttccatagc gtggccagag
    41941 aaaaaataat tttcagcaca ttctttcaca tgatttcagt aaatcat
//
LOCUS       DAZGOB010000029        41943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGOB010000029 DAZGOB010000000
VERSION     DAZGOB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.029"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(51..1448)
                     /gene="ccp"
                     /locus_tag="ACHLIF_001642"
     CDS             complement(51..1448)
                     /gene="ccp"
                     /locus_tag="ACHLIF_001642"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO9605675.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1853..3502
                     /locus_tag="ACHLIF_001643"
     CDS             1853..3502
                     /locus_tag="ACHLIF_001643"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO9605676.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3553..4155)
                     /locus_tag="ACHLIF_001644"
     CDS             complement(3553..4155)
                     /locus_tag="ACHLIF_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO9605677.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4675..5574
                     /locus_tag="ACHLIF_001645"
     CDS             4675..5574
                     /locus_tag="ACHLIF_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9605678.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5623..6636
                     /gene="yhjD"
                     /locus_tag="ACHLIF_001646"
     CDS             5623..6636
                     /gene="yhjD"
                     /locus_tag="ACHLIF_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO9605679.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7047..8369
                     /locus_tag="ACHLIF_001647"
     CDS             7047..8369
                     /locus_tag="ACHLIF_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9605680.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8603..10663)
                     /locus_tag="ACHLIF_001648"
     CDS             complement(8603..10663)
                     /locus_tag="ACHLIF_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO9605681.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10733..11500)
                     /gene="pdeH"
                     /locus_tag="ACHLIF_001649"
     CDS             complement(10733..11500)
                     /gene="pdeH"
                     /locus_tag="ACHLIF_001649"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO9605682.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11732..12661
                     /gene="kdgK"
                     /locus_tag="ACHLIF_001650"
     CDS             11732..12661
                     /gene="kdgK"
                     /locus_tag="ACHLIF_001650"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO9605683.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12757..14253)
                     /locus_tag="ACHLIF_001651"
     CDS             complement(12757..14253)
                     /locus_tag="ACHLIF_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO9605684.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14473..15759)
                     /gene="dctA"
                     /locus_tag="ACHLIF_001652"
     CDS             complement(14473..15759)
                     /gene="dctA"
                     /locus_tag="ACHLIF_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO9605685.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15942..17930)
                     /gene="hmsP"
                     /locus_tag="ACHLIF_001653"
     CDS             complement(15942..17930)
                     /gene="hmsP"
                     /locus_tag="ACHLIF_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO9605686.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSMLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18012..21485)
                     /gene="bcsC"
                     /locus_tag="ACHLIF_001654"
     CDS             complement(18012..21485)
                     /gene="bcsC"
                     /locus_tag="ACHLIF_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO9605687.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21467..22573)
                     /gene="bcsZ"
                     /locus_tag="ACHLIF_001655"
     CDS             complement(21467..22573)
                     /gene="bcsZ"
                     /locus_tag="ACHLIF_001655"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO9605688.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDIWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22580..24919)
                     /gene="bcsB"
                     /locus_tag="ACHLIF_001656"
     CDS             complement(22580..24919)
                     /gene="bcsB"
                     /locus_tag="ACHLIF_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO9605689.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24930..27548)
                     /gene="bcsA"
                     /locus_tag="ACHLIF_001657"
     CDS             complement(24930..27548)
                     /gene="bcsA"
                     /locus_tag="ACHLIF_001657"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO9605690.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27545..28297)
                     /gene="bcsQ"
                     /locus_tag="ACHLIF_001658"
     CDS             complement(27545..28297)
                     /gene="bcsQ"
                     /locus_tag="ACHLIF_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO9605691.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28309..28497)
                     /gene="bcsR"
                     /locus_tag="ACHLIF_001659"
     CDS             complement(28309..28497)
                     /gene="bcsR"
                     /locus_tag="ACHLIF_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO9605692.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28770..30341
                     /gene="bcsE"
                     /locus_tag="ACHLIF_001660"
     CDS             28770..30341
                     /gene="bcsE"
                     /locus_tag="ACHLIF_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO9605693.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30338..30529
                     /gene="bcsF"
                     /locus_tag="ACHLIF_001661"
     CDS             30338..30529
                     /gene="bcsF"
                     /locus_tag="ACHLIF_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO9605694.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30526..32205
                     /gene="bcsG"
                     /locus_tag="ACHLIF_001662"
     CDS             30526..32205
                     /gene="bcsG"
                     /locus_tag="ACHLIF_001662"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO9605695.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVSDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(32292..32399)
                     /locus_tag="ACHLIF_001663"
     CDS             complement(32292..32399)
                     /locus_tag="ACHLIF_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9605696.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(32775..32882)
                     /locus_tag="ACHLIF_001664"
     CDS             complement(32775..32882)
                     /locus_tag="ACHLIF_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9605697.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(33258..33365)
                     /locus_tag="ACHLIF_001665"
     CDS             complement(33258..33365)
                     /locus_tag="ACHLIF_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9605698.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(33741..33848)
                     /locus_tag="ACHLIF_001666"
     CDS             complement(33741..33848)
                     /locus_tag="ACHLIF_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9605699.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            34324..35595
                     /locus_tag="ACHLIF_001667"
     CDS             34324..35595
                     /locus_tag="ACHLIF_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO9605700.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(35625..36629)
                     /gene="dppF"
                     /locus_tag="ACHLIF_001668"
     CDS             complement(35625..36629)
                     /gene="dppF"
                     /locus_tag="ACHLIF_001668"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO9605701.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(36626..37609)
                     /gene="dppD"
                     /locus_tag="ACHLIF_001669"
     CDS             complement(36626..37609)
                     /gene="dppD"
                     /locus_tag="ACHLIF_001669"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO9605702.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDIYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(37620..38522)
                     /gene="dppC"
                     /locus_tag="ACHLIF_001670"
     CDS             complement(37620..38522)
                     /gene="dppC"
                     /locus_tag="ACHLIF_001670"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO9605703.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(38532..39551)
                     /gene="dppB"
                     /locus_tag="ACHLIF_001671"
     CDS             complement(38532..39551)
                     /gene="dppB"
                     /locus_tag="ACHLIF_001671"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO9605704.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(39702..41309)
                     /gene="dppA"
                     /locus_tag="ACHLIF_001672"
     CDS             complement(39702..41309)
                     /gene="dppA"
                     /locus_tag="ACHLIF_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO9605705.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(41661..>41943)
                     /locus_tag="ACHLIF_001673"
     CDS             complement(41661..>41943)
                     /locus_tag="ACHLIF_001673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605706.1"
                     /translation="SVASGNGTALIVGCGATALFDQHTTHFPANFAPHISLSINGFVT
                     ETIVFFYICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         9778 a        11529 c        10585 g        10051 t
ORIGIN      
        1 ttatcgtaat aaaaatcgcc caaacattcc tgttcgggcg atcaaattaa ttattgttta
       61 tcctgcatat acggcgtgta caccccgttc agactgtgca ggaaagctac gatatcatcc
      121 acatcctcct gcggcagctc tttgcctacc tgatagcgca gcatcagttt caccgccccg
      181 tccagcgtcg gcacgtcacc gcgatggaag tacggtgccg ttaaagcaac gttacgtaaa
      241 ccgggtactt tctgacgcaa tttatcacgc tcttctttag tcacattcat acgaccaata
      301 tccgccgccg taatttcccc aaagttaaag tcttttttca gccccaacgg ttcaaaggaa
      361 cgtccgccga gaataatacc accatgacaa gttgcacatt tattatcttt aaataattga
      421 tagccttttt tctgttgcgc cgtcagcgca ttttcatctc cacgcaacca tttatcaaat
      481 ggggaatccg gcgtaattaa tgttttctca aattcagcaa tggcatcagt aatattttcg
      541 ccactgaaac cttgcggata aacttcgagg aactgcgctt taagctgcgg atctttttcc
      601 agcttagcaa taatttcgtc ccaggatttc gacgccattt caatcgggtt caacggcggt
      661 ccacctgcct gatcctgcaa tgttgccgca cgaccatccc agaactgctc aacgttaaat
      721 actgagttaa ataccgtcgg cgcgttaatc ggcccaactg cgccaccaac accaatcgat
      781 gtttttctgc catcgacgcc ccccgcattc aacgcatggc aatgagcgca tgaaatggtg
      841 ctatcagccg ataaacgggg atcgtgatac agcgcaaagc ccaacgccac tttttgcgca
      901 tcggtaggca gtttttgcgg gatgggctgc accggttcat tgcgatgctc cggagcagta
      961 tcattgctgg cgtaatattc cgcgcgctgt tttgcaatcc aggccagtat ttccgcccgc
     1021 tcttcatcac tcaccttacc cgcccagtgt agcgcggtat aacgcgttgg tggcatagtt
     1081 tcatactgca tcacccattc aatcttattc agatcgcttt gcgaaacggg tttatcagcc
     1141 agcagtgccg cacgcacggc ctcgaggtta aaagatttat atccaagctt aatgtcgtaa
     1201 tccatcaact gtttcgcgcc aggaatataa taataggcgg gtaattctgc cgaaggcgtg
     1261 tggcaatagt cgcatccttt ttcgcggaga aagcctaaaa ccttattatt ttcactgaca
     1321 gcagatgcct gaacatcggc ctgtttactg cgtttattat cgtggtacca cacataacca
     1381 gataacccta aatagcaaat cgcgacgcca gccaggccga tcgcggtaat acgtgagacc
     1441 attttcatta ttcttccctc acggtggtga gttattgtga cccagaaaat tttccagaat
     1501 gctgcaaacg tctctgcgag acaaagccat cataaaaaac agggagatac gctttttgat
     1561 aggaatcaat caattacatg aatataaact atcaataaga tagcctgtgc ctattaccca
     1621 gatcaggaat ggcatatcat gattgcggcg agagcaggat tgaatgttct gaaaatgaaa
     1681 aacaacacga aatccgtatt tttataaatt ccattgatat tagtgcgtta tattttttga
     1741 caagttaata aacgtgatcc accgcacgct ttgtcgccca ccaggcggag cgaatgacta
     1801 cccttaaaga aaagcccgat aattagcgac gaatttcgga ggttggatcc ttatgctcaa
     1861 tcagaaaatt caaaacccta atccagacga actgatgatc gaggtcgatc tctgctatga
     1921 actggacccg tatgaattaa aactggatga gatgatcgag gcagaaccgg aacccgagat
     1981 gattgaaggg ctgcccgcct ctgatgcgct gacgcctgcc gatcgctatc tcgaactgtt
     2041 cgagcatgtt cagtcggcga aaattttccc cgacagtaaa acctttcccg actgcgcacc
     2101 caaaatggac ccgctggata ttttaatccg ctaccgtaaa gtgcgccgtc atcgtgattt
     2161 tgacttgcgc aagtttgttg aaaatcactt ctggctgccg gaggtctact ccagcgagta
     2221 tgtatcggac ccgcaaaatt ccctgaaaga gcatatcgac cagctgtggc cggtgctaac
     2281 ccgcgaacca caggatcaca ttccgtggtc ttctctgctg gcgctaccgc agtcatatat
     2341 tgtcccgggc ggccgtttta gcgaaaccta ctattgggat tcctatttca ccatgctggg
     2401 gctggcggaa agtggtcggg aagatttgct gaaatgcatg gccgataact tcgcctggat
     2461 gatcgaaaac tatggtcaca tccccaacgg caaccgcacc tattatttga gccgatcgca
     2521 accaccggtt tttgcgctga tggtggagtt gtttgaagaa gatggtgtac gcggtgcgcg
     2581 ccgctatctc gaccacctta aaatggaata tgccttctgg atggacggtg cagaatcgtt
     2641 gatccctaat caggcctatc gccatgttgt gcggatgccg gacggatcgc tgctcaaccg
     2701 ttattgggac gatcgcgaca cgccgcgtga cgaatcctgg cttgaggacg ttgaaaccgc
     2761 gaaacattct ggtcgcccgc ccaacgaggt gtaccgcgat ttacgcgcgg gagcggcctc
     2821 aggttgggat tactcttccc gttggctgcg tgatactggt cgtctggcga gcattcgtac
     2881 cacccagttc atccccatcg atctgaatgc cttcctgttt aaactggaga gcgccatcgc
     2941 caacatctcg gcgctgaaag gcgagaaaga gacagaagcg ctgttccgcc agaaggccag
     3001 tgcccgtcgc gatgcggtaa accgttacct ctgggatgat gaaaacggca tctaccgcga
     3061 ttacgactgg cgacgcgaac aactggcgct gttttccgct gccgccattg tgccgctcta
     3121 tgtcggcatg gcgaaccatg aacaggccga tcgtctggca aacgccgtac gcagccggtt
     3181 actgacacct ggcgggattc tggcaagcga gtacgaaacc ggtgaacagt gggataaacc
     3241 caatggctgg gcaccgttac aatggatggc aattcaggga tttaaaatgt atggcgatga
     3301 ccttctgggt gatgaaatcg cgcgcagctg gctgaaaacg gtgaatcagt tctatctgga
     3361 acagcacaaa atgatcgaga aataccatat tgccgatggt gttccccgcg aaggcggcgg
     3421 tggcgagtat ccgttgcagg atgggtttgg ctggactaac ggtgtggtac gccgtttaat
     3481 tggtttgtac ggcgaaccat aatattttta cagccagccg ctaacttcct gctggctgta
     3541 aaattatcct cttcaggagg agatatttaa catcattgcc gcctgggtgc gatttttcac
     3601 ttccagacgg cgatacaggg attccagatg cgcttttacc gttccggtac tgatattcag
     3661 cgctctgccg atctccttat ttgattcgcc cgccgctaac atggttaaaa tctcccgctg
     3721 gcgggcgctt aacgatttga gatctttaat gtccttttcc ggcgtcgtcc gccagtctcc
     3781 aggcagaaac atcatcccca tcgccgcact atttaccgcc aacgcaaatg tctcgacggt
     3841 tgaatcacga ggcacaatgg ccagcacatt aaaatggata acttcctgta accaccgttt
     3901 attgcaatcc gtcgccgtga ttaacacctt aacctcagga aactgcacca cggttttttg
     3961 cagcaaccag tagcaaaact caccatcctg atcgccatcg agcataacta aggcttcagg
     4021 gtaactttcc agcttttgcc ataactcgtc tgcctgactg gccccctgaa tactcactcc
     4081 tggaatacgc tgctgtaaac tgattttcat tccatgaata aatattgact gcctgtcaaa
     4141 catgactatt tgcataactg aatctccacc tgaatacgtt aaaaagactt aagtagtgga
     4201 agggtattac ccgcgagaaa aaataagaat tcgccatttg gcggtggcca ttctacagag
     4261 atgacgcgta gaaaatagtt accgatataa atagttacag ctaaacgcct gaaattacat
     4321 gtcgagggca ctatttaaaa caattttgag gatttcctta tattggtggt tagtacgcat
     4381 gcaattaaaa atgaaactcc gcgaccacaa gcaaaaataa caaacggcaa tgagacaaaa
     4441 ataagcacaa atagccaaca cgtcctctat tcactttaaa gggaatcgct gaaaaatacg
     4501 ctctgtttag ggggattcac ctttctcaga aagctattcc gcccttttcc tgctgagaaa
     4561 tcgccacatt cgtcatgaca acattgtgaa acccggcatt agatgttaga aaaaacaata
     4621 aacaatgcga tatgcgcgtt actgtttcgc tgtgaacaac aatcggtcaa agaaatggat
     4681 aaaattcacg caatgcagtt gttcatcaaa gtcgcggagc tggaaagttt ttcccgcgcg
     4741 gcggatttct ttgctttgcc aaaaggaagt gtttcgcgcc agatacaggc actggaacat
     4801 caacttggca cccagcttct ccagcgcacc acgcgacggg tcaaactcac gccagaaggc
     4861 atgacctatt atcaacgagc aaaagatgtg ttgagtaatc tcagcgaact ggacggtctg
     4921 tttcaacagg atgccaccag tatcagcggt aaattacgca tcgacatccc gccaggaatc
     4981 gcgaaaagcc tgttactgcc gcgcctgtcg gaatttctct atctgcatcc gggaattgag
     5041 ctggaactga gtagccatga ccgtccggta gatattcttc atgatggttt tgattgcgtg
     5101 atacgcactg gcgcgttacc ggaagatggc gttatcgccc gtcccctcgg caaactgacc
     5161 atggtcaact gtgccagtcc gcactatctg acgcgctttg gttatccgca aagccccgat
     5221 gatctgactt cacacgcaat agtgcgttat acaccgcacc tgggtgtaca tccgttaggt
     5281 tttgaggttg ccagcgttaa tggcgtccag tggtttaagt ctggcggcat gttgacggta
     5341 aacagtagcg aaaactatct cgccgccggt cttgccggtc tggggattat tcagatcccg
     5401 cgcattgccg tgcgcgaagc cctgcgtgcc gggcggctta ttgaagtgtt gccaggctac
     5461 cgtgccgagc cgctctccct ttcgctggtt tacccgcagc gtcgggagct ttcccggcgt
     5521 gtaaacctgt ttatgcagtg gctggctggc gtaatgaaag agcacctgga ctgaccgact
     5581 atacttttta agaatgacca cgacagaagg acaaaagagc ggatgacgca ggaaaacgag
     5641 atcaaacgtc ccacccagga tctggagcac gagccgatta aacagttgga taatagcgag
     5701 aaaggcggca aagttagcca ggcgctggaa accgtcacca ccactgccga aaaagtccaa
     5761 cgccaaccgg ttattgcgca cctgattcgt gcgacagaac gctttaacga tcggctgggt
     5821 aaccagtttg gtgcggctat cacctatttc tcatttttgt cgatgatccc gattttgatg
     5881 gtgtcgtttg ccgcaggggg ctttgtgctg gcctcccatc cgatgttgct acaggatatc
     5941 ttcgacaaaa ttctgcaaaa catcagcgat ccgacgctag ccgccacgtt gaaaaacacc
     6001 atcaacaccg ccgttcagca gcgtacgact gtagggcttg tcggcctggc ggtggcactt
     6061 tattccggca tcaactggat gggtaacctg cgtgaagcga ttcgtgccca gtcgcgcgat
     6121 gtctgggaac gctcgccgca agatcaggag aagttctggg taaaatatct gcgtgatttt
     6181 atttcactga ttggtttgtt aattgcgctg attgtgacgc tgtcgatcac ctcggttgcc
     6241 ggttcggcgc agcaaatgat tattagcgcc ctgcacctga acagcattga gtggctgaaa
     6301 ccgacgtggc ggttgattgg cctggcgatt tccatcttcg ccaactatct gcttttcttc
     6361 tggatcttct ggcggttgcc gcgccaccgt ccgcgcaaaa aagccctgat tcgcggaaca
     6421 tttctggctg ctattggttt tgaagtgatt aaaatcgtga tgacctacac cttgccatcg
     6481 ctgatgaaat ccccctctgg cgcagcattt ggttccgtgc tggggctgat ggcatttttc
     6541 tacttcttcg cccgtttgac gctgttttgc gcggcgtgga ttgccaccgc cgaatataaa
     6601 gacgacccgc gaatgccagg gaaaacgcag ccttaaaata ggccggatgc ggcgtaaacg
     6661 ccttatccgg catacgattt tattcggcct ggctccccgt aggccggata agatgcgcca
     6721 gcatcgcatc cggctataat gcgcacataa cctcttgaaa ctcttcccca gagcctcttc
     6781 agccatctat ttgggagcaa acaatttcat tccaactcat aaccccagca tataaatcca
     6841 gttggtaact tttatttaac ctgaaaccag ttttatccac tatttataaa attatgtgaa
     6901 gcatttcata gaagaaaaat cactggccta aacattatcc cctttttgcc tggtttttga
     6961 ccatttccgc gatttgttac acattgaaat atcacttttg ctgtgcgtaa tatggctatt
     7021 cgttagccaa aaaataagaa aagattatgc aagcaacagc cacaacactc gaccacgagc
     7081 aagaatacac gccgatcaac tcgcgtaata aagtccttgt cgcctctctc attggcacgg
     7141 ccattgagtt cttcgacttt tacatttacg ccactgcggc cgttattgtg tttccgcata
     7201 tcttcttccc gcagggcgat ccaacggcag caacgctaca gtcgctcgcc accttcgcca
     7261 tcgccttcgt cgcgcgcccc attggctctg ccgtttttgg tcattttggc gatcgcgttg
     7321 ggcgtaaagc gacgctggtc gcctcgttgc taacgatggg gatttcgacc gtggtgattg
     7381 gtctgctgcc gggctatgcc acgattggta ttttcgcccc gctgctgctg gcgctggctc
     7441 gatttggtca gggtctgggc ttaggcggtg aatggggcgg cgcggcgctg ctggcgactg
     7501 aaaacgcccc accgcgcaaa cgtgcactgt atggctcctt cccacagctg ggcgcaccga
     7561 ttggcttctt ctttgccaac ggcactttct tgctgctttc ctggctactg accgacgagc
     7621 agtttatgag ctggggctgg cgtgtgccat ttatcttctc ggcggtgctg gtcattatcg
     7681 gcctgtatgt tcgcgtgtcg ctgcatgagt cgccggtgtt tgagaaagtc gctaaagcga
     7741 aaaaacaggt gaagatcccg ctgggtacgc tgctgaccaa acatgttcgc gtaaccgtac
     7801 tgggtacgtt cattatgctg gcaacctata cgctgtttta catcatgacg gtctactcta
     7861 tgaccttcag taccgccgcc gcgccagttg ggcttggcct gccgcgtaac gaagtgttgt
     7921 ggatgctgat gatggcagtt attggttttg gcgtgatggt gccagtcgcc ggattactgg
     7981 ctgatgcctt tggtcgccgt aaaagcatgg taatcatcac cacgctgatc atcctgttcg
     8041 cgctgttcgc ctttaacccg ctgctcggtt ctggcaaccc gattctggtt tttgccttcc
     8101 tgctgctggg gttaagtctg atgggactga ccttcgggcc gatgggcgcg ctgttaccag
     8161 agctgttccc gacagaagtg cgttacaccg gagcatcgtt ctcttacaac gtagcgtcga
     8221 ttctcggggc ttccgttgca ccatatatcg cagcctggtt gcaggctaac tacgggttag
     8281 gtgcggtggg gttatatctg gcggcgatgg ctggcttgac gttaatcgcc ctgctgctga
     8341 cccatgagac gcgacatcag tcgttgtaag tatcgtgtgg gcagccaccc tcaccccagc
     8401 cctctccctg aaagggagag ggagaaaagc ggagcgagat gttatatcgc gaacataaca
     8461 gccctccccc agaaagtgaa cggaaaagcg gtactcgttg tttcaacacg cacaaaacag
     8521 tcccctcgcc cctttgggga gagggttagg gtgaggggaa cacgctgctc ccgaactcac
     8581 cagcaaacac aatccaacct catcacttct tcatctgcga caatatcgtc cgacactgat
     8641 tagcctcccc ttcggaaggt gagatcagtg ccagtaacgc cgccgccggt gttaccagcg
     8701 ttgccagtgc cgcagcaaca gcaccacggg caatcaacgg cccggctttc accccagcct
     8761 gcggattttt aaacgtccca cgcacataca gcggcgaacg cagtgtgata atccgaattc
     8821 ctttactctc cggatcaata gtcaaatcca gctgttcgga agcaaaactt gccgtgccgg
     8881 taacgttaat caacgcgttc tcagtatcga aagcaaagat ctgcgggcgc gccacgccgt
     8941 tggcaatatc cagattcgcc gccgcgcagt tcacccgcac ctcatcatca ccaaatatcg
     9001 caccgacaat gtagttgccg acattcagcc caacaatctc catcaggttg cggctcacca
     9061 gcccgtcatt catcaacagt ttcaggttgc cgttactgtt gcctaaaagt gccgccaccg
     9121 agttaccgct accgcgtagt tccgcgtcac cgttcatttc ccccagcgtc ttctgcatca
     9181 gttccacatc gggcatcagt tctttcagtt tcagtcgacg agcctgaata tctgcccgcc
     9241 cctgcatcgg ctttttatcg ccttccagat gaatattcgc cgcaatgctg ccgcccgcca
     9301 tgccaaattt cagcggttgc aggcgcaggt cagcattttt gaggatgata tgagtagaaa
     9361 gatcactaat cggcaggcta ctgccatgct caatgcgccg ccctttgaag cgaacatcgg
     9421 catccataac gtcccattta tcggtttcga agcggtcata aggcagcact ttgcccgcag
     9481 gctgaacgct tttttcgccc ttcttctgtt cagaccgttt cgacttttct gcccctttcc
     9541 cggagtcaac gccaattaac ggtcccaggt ccgccagccg caattgccgc gactcgacat
     9601 caccttccag ttttggtcgt ggctttccgg tggtgtagac cagagaaccg tggatatcgc
     9661 tatcgccaat tcgcccatta aaaccgcgat aatcaaagac cgacgatttt tcagtgtcga
     9721 ttttcgctac cagccgacca tccgtttcaa acggcggggt atcgggcagc agaacgcccg
     9781 tcagttcata gagatcaccc agtgaatcgc cagaaaattt aagccgtaaa tcgacaccgc
     9841 ccatcttcat tggatcattc acgacgccat caaaagcaac acgggtgtta ccagagcgga
     9901 aatcagcctg taccggaaac ggcgtccctt cgccacgcag cgccagcata ccgcctattt
     9961 ttcccgtacc cgtgagcggt tcaccgttat aacgtccctg cgccttcagg ccaaaaacgt
    10021 aatcgcccac cttttcttta tccgctttac ctttcgatcc agtaacttcg ctgaacggca
    10081 gcggcttgcc taacggatca acaaaaatct ccagatccgc tttgcttact ttgtcatcaa
    10141 tggcgatccg cccttgatcg aaaagaatat tatccagccg aaacgaccat gccgacggct
    10201 ttgcattcgc gtctttgtta tcatcgttgg cgagattaaa cgtccagtta ttgttctttt
    10261 cggagaggcg aatcaggcgc gcgtcgggct tttcgagctt gatccacggc agccagaccg
    10321 ttttggtcag cagcgccagc ggggccagcg ttgcctctac gcgtggcaaa tgcaccatcg
    10381 tgacttcggg aatatccggt ggattgccaa gaatgatgtc ttccgcgtgt acatggggcc
    10441 acggcaccca gctgcgccag ccagtttctt gtttttgccg ctcccacacc acgcccagat
    10501 cgccacggat agcgaacgga cgattcaact ccgcagagac tttctggttg atggtcggtt
    10561 tgagtcgatt ccagtcaaaa gttgcaatca aaatgatcgc cacgacaatc aacaacaaga
    10621 aagccactga aatcgcagcg gttattttgc ctgccttgct catcgttcgc ttcctcatat
    10681 tcttcctgtg ccagtcctaa agatagtcca gccaggcgga aaatgaggca gcttatagcg
    10741 ccagaaccgc cgtattcagc gtttctatcg gtgccgggcg tgaaagaaac cagccttgtg
    10801 cggcgaatgc gggcgagttc tgaacatcac gccactcttc cggcgtttct acgccctcga
    10861 caatcacccc gcgacaatag cgattcatta gatgtaaaag ctgagaaaag agtgtgcgtc
    10921 cttccggcga ctgacgcagc atcacaaaca gttctcgcgc gattttgatg tagtcataac
    10981 gcacttcact tagtgcagag aaatttgcca tcccggtacc aaaatcatcc agccacagcg
    11041 ggccaaattc acacatcgag gcaaaggttg aatctttcgg cagtcggata tgctccacca
    11101 gttcgaaacg cagccaggga agacgctcaa tctggcgcag gatttttggt tgctgacgca
    11161 gggcgatgag cgtagggcca tcaatattga ccgatgccag caggccgtgc tctataaaga
    11221 agtcggcttt ttgcgccagc aaatcaatct gctctttcac aacctccatc cgatggctga
    11281 cggtgatttc agtaaaatag cgatccggcg gcaggcgttg cgaagggttc aagggatgcg
    11341 tgaccaccgt taatagctcc acggccatta accgtccgca tgtttgatag atcggctgcc
    11401 aggtgtaagc acgctcacac tgcaaccaaa aacgccgttc ctgcaagctc tcgatgcttg
    11461 cttcagggtt gcttattcgc tggataacct gccttatcat caaagatgtc ctgtgtttaa
    11521 aagtgatgct gcccggactc gacaaagatt atcggcgcgc aagggcagaa ctttaccgcc
    11581 tggccggggt aaatttcctc actccacaac ggaatctttc aggaatacgt tctggctcaa
    11641 aaaaataatg gaacactgtt ttaatatggt tgaccagcaa accaccacag cgcaaactaa
    11701 cgctaatttt ttacagatca ggttcacgac tatgtccaaa aagattgccg tgattggcga
    11761 atgcatgatt gagctttccg agaaaggcgc ggacgttaag cgcggtttcg gcggcgatac
    11821 cctgaacact tccgtctata tcgcccgtca ggtcgatcct gcggcattaa ccgttcatta
    11881 cgtaacggcg ctgggaacgg acagttttag ccagcaaatg ctggacgcct ggcacggcga
    11941 gaacgttgat acttccctga cccaacggat ggaaaaccgt ctgccgggcc tttactacat
    12001 tgaaaccgac agcaccggcg agcgtacgtt ctactactgg cggaacgaag ccgccgccaa
    12061 attctggctg gagagtgagc agtctgcggc gatttgcgaa gagctggcga atttcgatta
    12121 tctctacctg agcgggatta gcctggcgat cttaagcccg accagccgcg aaaagctgct
    12181 ttccctgctg cgcgaatgcc gcgccaacgg cggaaaagtg attttcgaca ataactatcg
    12241 tccgcgcctg tgggccagca aagaagagac acagcaggtg taccaacaaa tgctggaatg
    12301 cacggatatc gccttcctga cgctggacga tgaagacgcg ctgtggggtc aacagccggt
    12361 ggaagacgtc attgcgcgca cccataacgc gggcgtgaaa gaagtggtgg tgaaacgcgg
    12421 ggcggattct tgcctggtgt ccattgctgg cgaagggtta gtggatgttc cggcggtgaa
    12481 actgccgaaa gaaaaagtga tcgataccac cgcagctggc gactctttca gcgccggtta
    12541 tctggcggta cgtttgacgg gcggcagtgc ggaaaacgcg gcgaaacgcg ggcacctgac
    12601 cgcaagtacc gttattcagt atcgcggcgc gattatcccg cgtgaggcga tgccagcgta
    12661 aacgcagcat atgcaaaagg ctggataaag tgttacccct ctatccagct catccataaa
    12721 tacctgatgg ttgtgctaac tcatcaccat caggcattac tgtgcaggtg gaatatccgt
    12781 cacttcagga tgtacgtcat ccgtggcgac agaggtggcg gcagcagcag tcgatggggc
    12841 catgatttga tcccaggccg cctgcaacgc tttcatgtta aattccggct cgcctttcgg
    12901 ctgcagcagt attaacgcca tatcattcga caactgctga cgcagatcct gatttaacat
    12961 ctccaccgtc aggctgttaa ggaaatcctg ccgcagtttc tgatactgct ccggcgcgat
    13021 atcgacgacc tgattttgca acgaacgcat ccgctgaccc atcagaatat cggtatcagc
    13081 ccgcgcatag gcggcaaaca gtttctgcag ctccagtttc ttttgcgcca ctaacgcatt
    13141 gaactcttct tccggcagac ctttatcgcg aaccttcgcc agttcacgcg ccaccagatt
    13201 aaggttgctg ttcagcttgt cgtttggtga ttcgatgttg atggcacact gcgcacgcag
    13261 atacagcaca cggcagtcaa atccaagacc gatgtctttg ctgttactgg cgcttaacgc
    13321 ttgctgaaca tgccagaaca gcgcctcgcg ggccaggtcc gcacgccagt agcgcagcag
    13381 tgcggctgat tcacgaatcg gctgccacgg cgtatcccac atgatagata accggtcctg
    13441 acgcaccgcg tcagtcataa tactcaccgc ttccgcacgc agcggagaaa gcgtcggcac
    13501 cggagctggc gtttcacgtt tgcctttcag ttcgccaaac gttttgttta tttggtcgac
    13561 aaccgagcgc gcatccacgt ttcccaccac cagtagcgtc attgcatccg gggtgtacca
    13621 tttctgatag aaatctttaa ttttttccgc ttcaacgggt tgtttcagcg gatcggcagg
    13681 atcgtgacct aacaaggttg accctttcag acgatagcgc caccagccct ctttagtatc
    13741 ggcaggccag gttgccacca tgtcctgact ttgcagcgcg tggttgatgg tttctggtgt
    13801 gatggtcaat ttgccagtgg catttgccag ataagagagc gcttctttca gcaaatcgtt
    13861 acggttattg ggcaaactca gattaaacag cgtggtgtca taagagacaa ttaccggcgg
    13921 catcgggcgt ttagggtcga tcccctgctg ccacaatgaa cgcgcctgtg ctgcgtcaag
    13981 gccaccgctt tgcgttagcg caatacgagg gatggcgtga ctgtaaccgc tctgttgtgt
    14041 actttcggcg agcgaaccgg tattaaccag caggcgaatt tcaacacgat cgctgggacg
    14101 ctggggggtg gtcagcactt gccactgtaa accgttggaa agcgtcccct gttgccatgc
    14161 tggatcaggc tggagcgcat ctgcctgcac atagccagca gtggccatca tcagcaaacc
    14221 gcccgctaaa agtcgaattt ttgtgccctg catgtgaacc cctgatcaac tatcctggta
    14281 ataaaaaact gcccgttgtc ggtcagtcta aatgacgtta aaaacacttc gtgttagacc
    14341 gcaagaacat gaaaatgtca cggaagaagt gaaataaacc tgaactcacc caggtggtaa
    14401 gttcaggcac aggggtcaat tatgcgcaaa cacccgcact cggggaaggg agtgcgggca
    14461 taagtgatga gattaagagg ataattcgtg cgttttgcca tccggcgcac gattattcag
    14521 cacatcgtcc agttttttgt ggtccagttc tttcacccac ttagcaacga caatggtcgc
    14581 tacgccgtta ccgaccaggt tagtcagcgc acgagcttct gacataaagc ggtcgatacc
    14641 gaggatcagc gccagacccg ctaccggcaa atggcccacc gcagagagcg tcgccgccag
    14701 cacgataaag ccactacccg ttacccctgc cgccccttta gaagaaagca gcaacacgat
    14761 taacagcgtg atttggtgga cgatatccat ctgactgtta gtggcctggg cgataaacac
    14821 cgccgccatt gtcaggtata tcgatgtgcc atcaaggtta aacgagtagc ctgtcgggat
    14881 gaccagcccc accaccgatt tgcggcagcc gagtttctcc atcttgtcga gcatacgcgg
    14941 cagcgccgac tcggaagatg aagtccccag tacaatcagc agttcttcac ggatgtagcg
    15001 gataaatttg aagatactga aaccagtcgc tttagcgatt gaacccaata ccagcaccac
    15061 aaacaggata caggtaatgt agaaacagat aatcagctgc cccagttgca ccagtgtgcc
    15121 gacgccgtat ttaccgatgg taaacgccat tgccccgaac gcaccaatag gcgccagacg
    15181 catgatcata ttgatgatgc cgaagatgac ctgcgagaaa ctttcgatga cgttaaaaat
    15241 cagttggcct ttgctgccca gacggtggag cgcaaaacca aacagtacgg caaacagcag
    15301 cacctgcaga atgttaccgc tggcaaatgc gccaatgacg ctcgccggga tgacatccat
    15361 aatgaaggcg acaatgccct ggtctttcgc ctgatcggcg taaaccgcta ccgctttcgc
    15421 atcaagcgtt gccggatcga cgttcattcc ggcaccaggc tgcacgacgt taacgatgat
    15481 aagaccaata atcagcgcga tggtactgac aatttcaaag taaagcagtg cgactgcgcc
    15541 ggtacgaccg accgccttca tgctttccat gcccgcaatg cccgttacga cggtacaaaa
    15601 gatgacagga gcgatgatca tcttaatgag cttaacgaag ccgtcgccaa gcggtttcat
    15661 ttgctcgcct atttcaggat agaaatggcc aaggagaata ccaatggcta tcgctgtcag
    15721 gacctgaaag taaaggcttt taaacagaga ggttttcata gggtgtcctt tagtaaaacc
    15781 acaggtcttg taaggttatg gggtacctgc ggccttaaaa taacacccag acaacatcac
    15841 agaaatgtac ctggatcata attgaaacaa aaaggttaaa aagtttgagc tggctcgcac
    15901 aaaccagcac tttttaaagt tttgtaatca gtttggggta gctacttttc ttccaggtaa
    15961 ctctcttcga agatttcaat agggagtggg cgagcaaaaa ggaagccctg ggcaatacca
    16021 acgcccgctt ttgccagcca gtcgcgttgt gcttcagtct ccacgccttc ggcaatcatt
    16081 tgtaagttca ggctctgcgc cagcatgata attgcagcaa tcatgctgct atcttccggc
    16141 aagccttcaa caaacatttt gtcgattttc agtacgtcga ttggcaacga tttcatatgc
    16201 tgcagctgac gcagccctgc gtagcccatg ccgaaatcat ccagcgccac ccgaactccg
    16261 gcattgcgca gcggacggag gattgccacc gcagcatgag ggtcgtcaat acgtcggctt
    16321 tctgtcactt ccagaatcag tgttcccggc tgaatgcgat agcgggttaa cagttccagc
    16381 atatccgcca ccatatttgg gtgcatcagt tgcagcgcag agaggtttac cgacaagggc
    16441 agcataatgc cgcgctcttg ccaggctgca agcaatcgac aggactcttc cagcacccag
    16501 tgaccgacgg taaccatcag cccacagcac tcaatgcgat cgattaagcc atccggcagg
    16561 tcccaactgc catccggttg ctggatacgc agtaacactt ccgcactgac cagttttccg
    16621 ctggtcatct cgacctgtgg ctgtaaccaa atagcaaact gatgattttc cagtgcatta
    16681 aggatatcgc tctcttccgt caaccgcttc tgggcggctt ccatctgctg cggatcaaag
    16741 aactgaatct gattcttgcc tttatggcga gcggtaaatg ccgcagaaat agcgcgactg
    16801 taaagctgtt cggcggtgag atcgccgtag aacatcgcca cgccaatgct acagtgcgga
    16861 cggagttgaa tacgttcaat cggcaggcgc tcgctcatga tagtgagcac ttgctgacct
    16921 aaggtgattg cgtgccacgg ttcctgtaca ccgttggcaa tgacagcaaa gtcataaccg
    16981 ctaatctgcg cgaggatcat acgtggcgac agtaccgatt tgagtttttc caccagcgtc
    17041 agcagcagaa tttctcgttg cgcctctttc agcacgcccg cagtatcacg cagggtttca
    17101 caggtgatga tcatcagcgc ggtggtttgt ttacgcgcga caacctgctc cagcatctcc
    17161 atcagcaagg ctttgttcgg caaatccgac accgggaagc gcatcgcatt ttcgttctgt
    17221 tcttcataat ggcgctgcag caattgctgg ttgaggttgt aactgcgcac caacataccg
    17281 atttcatcgt cctgatgcag acgcggtaat gccagttggt gaccaacaag ctcctgggct
    17341 gggatggcgt taagttcgcg agcaatattg cgtaacggat gcaaaatcag gcggttaatg
    17401 caccagctga tggcgacggt caggataagc gacaaaagta agtaaatggt cactaacatt
    17461 gagagggtgc tcatcacgaa cttatacata cggaaggaat ccgcctgtag taccagatag
    17521 gcaattggct gcgggtttgc cggacgttcg agcgagtaaa cgcccagcga gatttgaacc
    17581 ggtagctcaa acaggcgagt aaccattacc ggcaccgggc gctctggaat aaaactttta
    17641 cgcagcgcct ggaactggtt aggcagcact acatcggcac ggctgaccac gcctgccggt
    17701 ttaatgctgg caagaatagc ttccgcttcg ggaatatcgc ctttcaaaat agctgaagat
    17761 aagggttcgc ggacagagcg agcaatgctt tccagttgcg tagccgtgtt atagcgattc
    17821 tgttggacca gatggaacag caaaacggtg caaaaaataa aaacgaacac caggacaacg
    17881 gctgccacca ttgccatctg cttgattgtt aacgagcgac ttacgcgcaa attgtctccc
    17941 cccggaaatc ccagattgcc gcccgagtat acccgatcgc agcggcatta agagaggcgc
    18001 tatctgaaaa cttaccagtc ggcgtaaggt atcagcggct gcggcggtaa atccatgtca
    18061 ccctgccatc cggcggcgga ataacgtacg tagagcagga aatggctggg tgcgtaatct
    18121 ttcgcctgct ggatatcaat tgccgtgcca acaaaccagt tggaagtaac acgtcgttca
    18181 agtaatgccc gcgccgtgta gccgaagccc tgactgctgc cgccatcgtt ggattgccgc
    18241 gcagcttctt cctgccagtc ggtcgggatc agattcatca gcggataacg cggcatggtt
    18301 ttggtgcgtg aatgcgacca cgagccagac gcccccagct cccacgacca gttttccgtg
    18361 cgctcccgcc acatcaccgg tatggcaaac gacaggtatt cctgcggact gtagtaaccg
    18421 ccctgaccga gtgagtagcc actcagatct ttgtcgtaat gccagatcat gttgttcagg
    18481 ccgattgtga cgcggcgatt gttctggtta atgaccttat agtaatagcc cgtcatccag
    18541 cgcacgcgcc agttatcttc gacattttta ccggttaact ggtcgccact aagcgatgcc
    18601 cagacgccgt ttgcttcacc tttatcgtag ctcagactta gccccacacc gtcggcacgt
    18661 acgccacccc attttttccc ggtattgctc ggggagtctt tttgcccacc aaaggccagc
    18721 aaagaactgg agatgggccg acggtgggcg ttaacggtgt aacccagcgg cccgatatca
    18781 tcgctgtaac tgatgccgcc gaccacatcc accacgttga agcccatcgg cgtggtaccg
    18841 atatcccagc tccagacgtc atttcgccag ccgaccgcca cgctggcacc ggaatccgac
    18901 tggctgcggt tgccgctaca gtcctgtaat gtacaggtgc cccagttgtc atcccatttg
    18961 ccatcggcat tagtggagaa actgccgacg ttcatattga cgaaatcact gcgaaagaac
    19021 atccgcccgt cagaatacgg cgcatccacc tgcaacatgg tagtgtgcgc tttcagatcg
    19081 gagtaaccac cggtgccgct cgaaccccag taatcgtgct cgagggtgac gttaagatcc
    19141 tgctggcgat agaggtccgc cgcatcgctg cgcacgccgc gtttcagcca gtcatctttc
    19201 tcgtcgttac gggtcagtcg ggtgaatgtg tcgttatcct gcggacgcgt cgtagtcaca
    19261 ccggatgcga ccatggcgtc tttgtaggtt tccagcgcct gctttggatc gcccgcctgc
    19321 gcttcaaact tcgcaccgtc gcgcagcacc atcgcgcttt ccatcgacgg tggctgagat
    19381 tttgcctgcg ggatcaactt attaaacgtc tgctgcgccg ctgcggtatc tccaagctgc
    19441 gcctgcgcca gcgccacgcg ccgctgtgtg ttcagcgagg cgttatcggt agcgggcagt
    19501 ttcgccagct ggctacgtgc cgccgctgtg tcaccggcag caatatccac ttccgtcagc
    19561 cccagaatgg catcggcgtt agttggctcc agcgtcagga cattctgata tgcagcgcgg
    19621 gcggcggtgt aatcacgtcg ctgctgcgcc cagtcagcca gcgtgaggtc aatacgcgtg
    19681 gaaggtggtt gctgacgcag catcgcttcc gcttcagctt ctttgccgct ttctcgcagg
    19741 cgattagcgg tttccagcac ctgatcgctt tgcagtcgat taaccagctc ctgaatattg
    19801 ctgttccact gcgcgcgcgg caggctgttg atatgcgcca gcgccgctct gtcctggtca
    19861 tgaccagaga ggtacagccc gtaagcgtaa acctgctccg ggtcgttcgg cttctgctgc
    19921 gccagattgc gcattaacgt atcggcctgg ctgcgttgtc cggcctgcca gagatcctgc
    19981 gaaagtcggt aagtaatcca tacgctgccg gggtccagcg ccagtcgttg ccgctgaagt
    20041 gctgccgcct gcgcccattt gccctggttt tccagtgcct ctgcctgctg tgccagacgg
    20101 tcgttttgca ggctgcgttc gatatcatca atgctacgcc gctgactggc agagagcgag
    20161 gcgataaacg cttcagcttt ttctggcgat tgctggcggt aaatatttgc cagcccgcgc
    20221 acggcgttag tgttgccgct gtccatacgc aaggtctgct gataataacg ttcggcggcg
    20281 ggataatctt ttcgcgccat cgccacatcg cccagcccca gcactgcata actgtcggtg
    20341 ttatcgacat tacgcgcctg ctggaacagg cgttctgccc ggtcaggatt attggctttc
    20401 agcgcagcat cgccctgctg gatcgccagc cagtagcggt ttactttcag cagactgttc
    20461 catttgtcgt tgttgctgct gtgcgggtcc agtgcgaggg ctttttccag attcgccact
    20521 gcattggcgc gatcgccttt ctgagaatac gcctggccca gcgcccccag agcttcactg
    20581 tctttcgggt tcgcccgcac cgcctgttgt agttcgggaa tggctttacc cgccatacca
    20641 gagtccaccg ccgctaaacc ttgcgcacga gcgcggaaag caggatcggc cagctgtttt
    20701 tgctgttctg ccagttgcga ttgcgcagcc gccacgctat cgccatcact aaagatcgag
    20761 agatattttt tcagcgccga cacactggca tcactgacgg gcatgtcttt aatctgcccg
    20821 taccagattt tagaggcccc ttcgcgcccg gcgttcgatt ttgccatctg ttccaggacg
    20881 gcaaaacctt cgtcacggcg atcgctacta aacagcaata gcgccagatt gttttgcagg
    20941 cccgtattgc ccggtgcatc cgcattgatg cgttttaact gattaatcgc ttcgccacgg
    21001 cgagccggaa ttttcgccac cgtactccag tactcgacag caatgtcacc ttccggcggc
    21061 gcaccgttga acagtttgtt gtaactcgcc acagcttctt ctgcatgacc ggtcgtcgcc
    21121 tgcaatcgtg cctgttgcag tgcctgacga ccatccggcg tggaaagtag catcgtagtc
    21181 cgcgacgatt tatacgcatt tgaactcggc gctaactgcg acagccgatc gagctgtttt
    21241 tgcgcgccat caatatcgcc ctgacgtaac aaagaacgga aacgggcggc aacgacgtcc
    21301 gggttattcg gatcaataag ttccagccga tataacgact gttgcaccag atcttcacga
    21361 tgggtcgctt cgcctaaccg aacttgctcc agcaactgtt gctgagcggt tggtgctgcc
    21421 tcgaccatcg gcatgacggc cagaccgagg gaaagcgtga atatgtttag tgtgaatttg
    21481 cgcattcctg gccccagtca ggtaataact caccttttgt cgagaagcgg aaacggtgtt
    21541 gatcccagcc ttgtccaaac agggtcagca catagttgta ataggcatcg ctgccgggaa
    21601 agttatcggc cacgcgctgg cgctgaacgg cctgcgcatc gcggttttgt aaaaagggca
    21661 gcatggcggc agaaaaaccg accggtcctt taccctgcgc tttccccgta gccacatcca
    21721 ctttttccgg cggataaccg tttttctcag tgaatgtcgc catcggttta aaccggttga
    21781 gcatccgcgc tttttgcgga tcgctgtcag gcatcatgcc tacccacatg taaacgcgga
    21841 tagcgtcgta gctgctgatc aatgtttttt cggcttttag ctgccagcct ttgtctttct
    21901 catagcgcac ccagtctggc gaaaaacctt tcggggcggt ttccagcaat aaacgttgat
    21961 tggtttcgcg cagcgtggtc cacggcgcgc caaagcgggt gaaatactgc gccagcgtcg
    22021 gcggcaggta gctggggtta aaacgccagc tgttatcctc agcaaaaccc actttgcccg
    22081 gtaacaacat ggaacccagc ccaggcaccg tcaccacttc ctcccgcgcg atacgtttta
    22141 gcaacgcgct gccgatgtcg gtataacgct gctctttcca caaacgcccc gcctccagca
    22201 acgaccaggc catccagata tcaccatcgg aggccgaatt gctgtccagc acttcccact
    22261 tactgttctc tttcttgccc cacagccagg cgggcaaacg ttcttttaaa gaaccctgag
    22321 cgagattgtt ctgcgtccag tcgagaatat tatcgaaagc tgcacggtcg ttagccgcca
    22381 gggcaaagaa catgccgtaa ctttgccctt cggaggtggt gattttgcgc gcgtcgctgg
    22441 ggtcgatgac gcgcccttcc tgactgatgt aatccttttt aaactgctcc caggcaggcc
    22501 aggtacaggc tgcctgaaca ctaaaggcag ccagcagcag catcgtcacg attccactac
    22561 gcaacacatt catcttcaat tactcgttat ccgggttaag acgacgacga ctaataattc
    22621 gcagcagacg ccacagtacc catgccagca atatcacact gatagccgcc agcaccgcca
    22681 gcagaatcgg atggtttgcc agcgcatacc acacgcgctc gaaccacggc agatgaccta
    22741 cgtaataaac gtcgccaaca cgtaggctgt tgataccgga ctcgcggatc accgcgaccg
    22801 aaccgaacat ggtggcgcgt ttgccgctat cgttcaccgc atcgttaagc atttcataac
    22861 cgcgtgggct atctgccaac agcgcaatca cgctgcgctg gtcgttatac ggcgactgga
    22921 agccaatcac cgccgccatc gcaccggaag aggtcagcgt tgaccgggtt tctgccgcgc
    22981 gatcgctctc gtccggcaca atgccgggga acggggtctg gcgcatcggt gttttcaccc
    23041 agctttcggt cgcctgcacc aataggtcga tctgcttatc gtctttcagt ttgtccggga
    23101 taccaccgat gatcatgatg tcggcatctt tgccctgaat ggtgctgcca tcatcggtca
    23161 ccgtcagatt aatcgccggg aagcccgtct gtgcgccgat aaaaccaaca gtattcagca
    23221 acgtttccat ctgtgcttcg ttaggcgctt tcggcatcac ggtgatggtt tgcgacagat
    23281 ccgccatccg gctgaatggg aagcccgcgt tagcaaaggc gcgtagatcc ggcatcggga
    23341 tgaagtggta atacttcgag aagtcgatgg tggagtcgtc accaatcacc acatgattct
    23401 gcaccggctg gaaggtaata cagttatcca ccgaaccgcc cggcatcggg ttcatatact
    23461 caaagtcgaa gcgcagctgg ttggtcgcgc ccagtttcag cgccggaata gagacatctg
    23521 ttttgccatc cagcaaacct tgtaataccg gaatccgcag cagcaggcgg ttcgcctcct
    23581 gtttgctgct cagattgaag gattgcagga actggttatt caggctgata tccatccgcg
    23641 aactgtcttt caccggcggc atggtgtagc ggtaattaat atccatatca atgccggtac
    23701 tgcgcatcag gtagagatcc ggcggcaggt ttagcgaaac gttaatcgct gctggctcaa
    23761 gaccgctgga ttgtaactgt tcttcatagg ttttcagttc gccaaatgtg accggacgat
    23821 cggtacgtac ccagttcggc gcatcgtacg gcttacgcgg tagcagcggt ttcacttcat
    23881 tcactaccac gctttcacca cggaacagaa tgttaccctg agcgatacct ttcgctgcct
    23941 gcaacaggtc tttgtcgtca cgaccaaaca ccaccagcag tttgacgtaa ggattctgcg
    24001 gatggttaat catctcaatc acaggggctt ttaccgccgg atgatcgcgc aggaagtccg
    24061 gccgtttgtc gttggttgca aagacaatgg cattacgatc cggcagttgg ttatagagca
    24121 ccgggaagtt ctgcccacgc cagccagaac gcgaaccaaa ccacgaggcg acaatggcag
    24181 aggcttgttg cagcccaaca tccggcgcac ccgcaaagac catcggcaag gtgttagtac
    24241 ggttatcgcg cgggtcaaag aatggcaccg ggaagtgtga caggtcattc ttcacattca
    24301 gggtctgata ggtcagatcc agtccactgc tccgcccaac atccagccaa agcgtggtgc
    24361 tggccgggtt ttcgcacacg tcctgataat ggccgacaaa ctccagccgc acacggttga
    24421 agtcggtaat aaacagtggg ttaatgggca tttgcgccag cgttttttta cccaactgtt
    24481 ctttggtcac tggcagcacg cccatcagct catcattgag ataaaccttt aactgcgact
    24541 ggacaggcag taacgatggc gatggcgtgt attcgaggtt gagcatcgcc ttcgtcacca
    24601 cttcatcgct gcgcatgcca aactcaatgc tgccgttcgg gttaatgcca cgtagcacca
    24661 tgctgcccgg cggcggcgca atttgcgcaa aggtcagctt cacatcacgc gaaggaccgt
    24721 tctgcgccac gactggcgca tcagcgccct gcacgcccgg catcacttgc cccacctgcg
    24781 gattttgttc cgtctgggcg gctacagctg gctcagcatt gatcagtggt tgcgttgctg
    24841 gcgtcgcctg cgtcatgaaa gaggggaacg cactcatccc catagccact gcacaaatcc
    24901 agaatagttt tcttttcatc gcgttatcat cattgttgag ccaaagcctg atccgatggt
    24961 tgtgccgttt cgctccgctc cgggcggcgc ggaataaacg atacaaccca ggaaaccaga
    25021 gaagtcagca cacggaatat gcccttcacc gaagaaggcg caaactccgc cagatggcgg
    25081 tagccacgga agccgagctt cagaatatcc agcagacttt ccagcggctt atcttccggg
    25141 tagctgtcct gccagagcgc ccatgtatcc gcacgggcaa acgtacactg cacaaaatcg
    25201 atatgttgct gggtggtgag cggcattaat ttcagcccaa cttcattacc catcacgcgc
    25261 gccacctggg tcgggaagac gtattcctgc tgaccgcgtt taagcaacag attcactttc
    25321 tgcccttcca gaatctgcgc ctgaccgttg atcttgatcc ccaaaccacc gtcggagaaa
    25381 tcctgaacgg tacacgagaa gaggtgacca tcttcgcggg caattgccgc gggcatcgtc
    25441 atctccacgc ggtgcgatcg gcgtacctgt ttgctttcta ccgatactgc aactgcgccg
    25501 ccaagaacaa tcaggttgta gaatacccac accatactga cgaccacggt gagcatctcg
    25561 gttggcgggc catagaagta gcgccagatg cctaccgcaa cgcccaccag gttgagcagg
    25621 acaaggaaga tgtagggccg cgagatcacc cagtcgacgt actcttcttc caccagtcca
    25681 cctttggcgg tgacgttaaa tttgcctttg tgcgggttaa tcagcgccac cagcgtcggt
    25741 ggtgcgatat accacgccag caccgtttcg tagatttcac tccagaaaga gtggcgatat
    25801 ttgccctgga tcttggagtt ggtcaggctg gcatggatca tatgcggcag cacgaacagg
    25861 gcgatcatca acgctggcgc atagatgatg taggcatgaa gcagcaggaa cgccagcggc
    25921 gcagtcagga agatcagtcg tggaatgccc gacaagaagt ggaacatggc gttgacgtag
    25981 cacagccgct gggcgaattt cagcccttta ccggtaagcg ggttatcgag acggaagatt
    26041 tgcaccatcc cgcgtgccca gcgaatacgc tgaccgatat gcgccgacag actttcggtc
    26101 gccagccccg ccgcctgcgg aatacgcata tatgcagagg tgtagccacg acggtgcagg
    26161 cgcagagacg tgtgcgcatc ttcggttacc gtttcgacag caataccgcc aatttcatcc
    26221 aatggtttgc gacgaattac tgcacaggaa ccgcagaaga aagtggcgtc ccacatatcg
    26281 ttgccatcct gcaccagacc atagaacagc gtgccttcgt tcggcgtttt acgaaaacgc
    26341 cccaggttgc gttcaaacgg gtccggcgag aagaagtggt gcggcgtctg catcatcgcc
    26401 agctgtttct ctttcaggaa ccagcccatg gtcatttgca ggaacgatcg cgttggtacg
    26461 tggtcgcagt cgaaaatcga cacgaactcg cctttggcat atttcagcgc attgttgatg
    26521 ttgcccgctt tcgcatgttc atgagtggta cgggcgatat atttcacccc cacgttttgc
    26581 gcaaactggc gaaactcttc cctaccgccg tcatcgagga tccagatgtt cagcttgtct
    26641 ttcggccagt cgatacccag cgaggcgtaa atagtatttt tcaccacgtt gagatcttcg
    26701 ttgtaagtcg ggacaaagat atccaccgac ggccacagcg acatatcttt cggcaatggc
    26761 accggctgac gattcagcgg ccatactacc tggaagtagc cgagcaccag cacaatccac
    26821 gcgtacgttt cagcgaagag cagaataagc ccgcacacca ggctgaccgg atcgtcccag
    26881 ttcagcgtag aggtgtaacg ccaccagata taacggcaag aaacggtcag cgacagcaca
    26941 atcaacatta gcgccgagaa gcgccccggc atccgccgta cgatcagcgc taccccccac
    27001 agcagcatca ggaagataaa ctgcgccagc gggttaaacg gctgagtaac gcagattaac
    27061 gccagaatca gcgagaaggt gacgatgata ccgaggatca accgccgcgc cccggcactc
    27121 aaatgaccga gctctttttt ctcatccaga tgctgtgttt tatggctaac gcgctcaggc
    27181 agctcgttca tccattgatg gtaacgtcca cgaatatttt gcagacctga aaatgcccgc
    27241 ctgcgcggtt tcggcgtttc tttgcgcgat gcaccgatca ataaccagca tgtttgaatg
    27301 agataacgga ccgggtccag cggacgcgga cgcgaggcgt tgatatgcgg atacaggttt
    27361 ttatgttctg cgcgaatacg ctgccagcgc gggtgctcca gcggaataaa aatccaggcc
    27421 aggatcatcc agaaacagcc gagcgtcgcg ctgaaagccg acgcaccgtg gcgacgataa
    27481 tcgcgataac gcccgataag ccgcgcgttg accggcggga taagcaacca ccgggtcagg
    27541 atactcatga tgcactcccg actggcgttt tcagcccgga atagttcaac aggcaccagt
    27601 tcgccagcgt cagtatctct tctgccgcca gcgcatcact gcggtattca ccgactggtt
    27661 gcttagccgc caggcattca gccatcgctt catcacgatg aatgagcatc ggcagcaatc
    27721 ggcgctggct ttgcaaccaa agctggtaaa tatcgtcctg aacctgactg ccaatacgga
    27781 agttattaat caaaatatgt gcgccatccg gcagcgcttg ctgatgcagg cggatatggc
    27841 agttggcatc cacattgacg atagccagcg agtgatcgca caaactcagc agctggtggg
    27901 ttatctgcga ggcatcacgc ggtaagtcga ttaaaatcca ctggtaacgc ccgctggctt
    27961 ttagttgctg taagccggag caaatatcgc tcagccgggt ttgccagtgc tgtggatttt
    28021 cttgttcttc aatggataac tgaccaaaag gcagcaaatc gagctgcgag gtgtagcgca
    28081 acccagcgtc acgccagtcc tggccatcca gcatcgctct ggcccagccc tgacggtggg
    28141 taaaatcaac attaaatgac aggcgcaaca agttgtccgg gcaggcatcg accaccagaa
    28201 cattttctcc caacatttgt aatgaccagg ctaatgcggc ggtgatggtt gttgtcccca
    28261 cgcctccccg caccccctgc aatcccagta cggccattca ccaatcccct acttttgttg
    28321 cgcaaactct gccagcaacg gccagcgttt taatgccgcg gccaactgtt cgcgttggga
    28381 aatatcggca taatcaatat caggcagtga aaatgcctgc tttaacgcca caatatcatt
    28441 ctggaagata tagcctatcg cgggatcagg cagagtatct ggttcattgt tattcatttt
    28501 gcttgatccc tatgaaataa ccgtaccgtt catgagacaa ttctaaccgt gagcatttcg
    28561 ccggtttatt gtttacggcg agacgggaat ttgctcactt tttgactttc cgttacgcaa
    28621 cagaaaaagt taaaaacgaa aagactaaaa tcacacgtct tgtgaccttc taaattgatt
    28681 cacatgctaa atctgataag ttttaatttc aatggtaggt ttatttctta gctttcgcta
    28741 gtaaactgat aatcagacaa aatggtgaca tgagggacat tgtggaccct gtattctcta
    28801 tcggtatctc atcattatgg gatgagctgc gacatatgcc agcaggcggc gtctggtggt
    28861 ttaacgtcga tcgccatgaa gatgctatca gtctggcgaa tcaaacaatt gcatcccagg
    28921 ctgaaaccgc acacgtcgcg gtcattagca tggacagcga tccggcgaaa atctttcaat
    28981 tagatgattc tcaagggccg gaaaaaataa aattattttc aatgctaaat catgaaaaag
    29041 gtctatacta tttgacccgt gatttgcagt gttctattga tccccataat taccttttta
    29101 ttcttgtttg cgcaaataat gcatggcaaa acattcctgc cgagcggctt cgctcatggt
    29161 tggataaaat gaataaatgg agcaggttaa accattgttc gcttttggta attaatcccg
    29221 gaaataataa cgataaacaa ttttcattgt tgcttgagga ataccgttca ctttttggtc
    29281 ttgccagttt gcgttttcag ggtgaccaac atttgctgga tattgccttc tggtgcaacg
    29341 aaaaaggggt cagcgcccgt cagcagctta gcgttcagca acaaaatggt atctggacat
    29401 tagttcaaag cgaagaggcg gagatccaac cacgcagcga cgaaaaacgc attctgagta
    29461 atgttgctgt actggaaggt gcgccgccgc tatcggaaca ctggcaactg ttcaacaata
    29521 acgaagtcct gttcaatgaa gcccgtaccg ctcaggcggc gacggtggtc ttttctttac
    29581 agcaaaatgc gcaaatcgag ccactggccc gcagcattca taccctgcgt cgccagcgcg
    29641 gtagtgcgat gaaaatcctc gtgcgggaaa ataccgctag cctgcgcgcc accgatgaac
    29701 gtttgttatt ggcctgcggt gcaaatatgg ttattccgtg gaatgcgcca ctctcccgtt
    29761 gtctgacgat gatcgaaagc gtgcaagggc agaagtttag tcgctatgtg ccggaagata
    29821 tcactacctt gctgtcaatg acccagccgc tcaaactgcg tggtttccag aagtgggatg
    29881 tgttctgtaa tgccgtcaac aacatgatga ataaccctct attacctgcc cacggtaaag
    29941 gcgttctggt tgccctacgt ccggtaccgg gtatccgcgt tgaacaagcc ctgacgctgt
    30001 gtcgccctaa ccgtaccggc gatatcatga ccattggcgg taatcggctg gtgctgtttc
    30061 tctcattctg tcggattaac gatctggata ccgcgttgaa tcatattttc ccattgccta
    30121 ctggcgacat tttctcaaac cgtatggtct ggtttgaaga tgatcaaatc agtgccgagc
    30181 tagtgcagat gcgcttgctt gccccagaac aatggggcat gccgctgcct ttaacgcaaa
    30241 gttctaaacc ggtcatcaat gccgagcacg atggtcgcca ctggcgacga ataccagaac
    30301 ccatgcgact gttagatgat gctgtggagc gctcatcatg atgaccatca gcgatatcat
    30361 tgaaattatt gtcgtttgcg cactgatatt tttcccgctg ggctatctgg cgcggcactc
    30421 tttgcgacgc attcgcgaca ccttacgttt gttctttgct aaacctcgtt atgttaaacc
    30481 ggccgggacg ttacgccgca cggaaaaagc cagggcaacc aaaaaatgac tcaatttacg
    30541 caaaataccg ccatgccttc ttccctctgg caatactggc gcggcctttc cggctggaac
    30601 ttctattttc tggttaagtt cggcctgttg tgggcgggat atcttaactt ccatccgctc
    30661 ctcaatttgg tgtttgccgc gtttctgctg atgcccattc cgcgctacag cctgcatcgc
    30721 ttgcgccact ggattgccct gccgatcggc tttgctttgt tctggcatga cacctggttg
    30781 cctggcccgg aaagcataat gagccagggt tcgcaggtgg cggggttcag taccgattat
    30841 ttaatcgacc ttgtcacacg ctttattaac tggcagatga ttggggccat ttttgtttta
    30901 ttagtggcct ggttattcct gtcacaatgg attcgcatta ccgtttttgt ggttgccata
    30961 ctgctatggc tgaacgtact taccctggcg ggaccaagtt tctccttgtg gccagccgga
    31021 caaccgacga ccactgtaac aacgacgggt ggtaacgcag cggcaaccgt tgcggcgacg
    31081 ggtggcgcac cggtagtgag tgatatgccc gcacaaactg caccgccaac aacggcgaac
    31141 cttaacgcct ggctgaataa tttctataac gcggaggcaa aacgtaaatc gaccttcccg
    31201 tcttcgctgc ccgctgatgc tcagccattt gaactactgg tgattaacat ctgttcgctt
    31261 tcctggtcgg atatagaagc cgccgggttg atgtcgcatc cactgtggtc gcatttcgat
    31321 attgagttca agaactttaa ctccgccacc tcctacagtg gcccggcggc gatccgttta
    31381 ctgcgcgcca gctgcgggca gacttcgcac actaatctgt atcaaccggc aaataacgac
    31441 tgctatctgt ttgataacct ttcgaaactg ggctttaccc agcacctgat gatggggcat
    31501 aacggccagt tcggcggttt tttgaaagaa gttcgcgaaa atggcggcat gcagactgaa
    31561 ttgatggatc aaacaaatct gccggttatt ttgctgggct ttgatggttc gccggtttat
    31621 gacgataccg ccgtgcttaa ccgctggctg gacgttaccg aaaaagataa aaatagccgt
    31681 agtgccacgt tctacaacac gcttccactg catgacggca accattatcc gggggtcagc
    31741 aaaacagcgg attacaaagc gcgggcgcag aaattctttg atgaactgga cgccttcttt
    31801 actgaactgg agaaatcggg tcgtaaagtg atggtggtcg tggtgccgga acacggcggc
    31861 gcgctgaagg gcgacagaat gcaggtatct ggcctacgtg atatccctag cccgtctatc
    31921 acagacgtcc ccgttggggt gaaattcttc ggcatgaagg caccacatca gggggcaccg
    31981 attgtcattg accaaccgag cagcttcctg gctatctccg atctggtggt tcgcgttctt
    32041 gatggcaaga ttttcaccga agacaatgtt gactggaaaa aactcaccag tgggttgcca
    32101 caaacagcac cggtctccga gaactcaaat gcagtagtta ttcaatacca ggataaaccg
    32161 tacgttcgcc tgaacggcgg cgactgggtg ccttacccgc agtaagcacg aaaaaggccg
    32221 cagagtttaa cctctgcggc ctgatccggg cgcaaattgc cattacggca gcctgacgcc
    32281 cgcatgacac gttacttccg cttattcagc cagttcacga tcatactggc aagaatgcca
    32341 gcaatgaccg gagccgctaa atcatgccag aaggccatgc ccagctctgc gagcgtcata
    32401 tagccgcctg tgttgtaatg acaacgtttc gcggctattc ttgagttgtt tgggttcaag
    32461 attagccccc gttatgttgt caggtgcata cctgcaacgt gcgggggttt tctctctcca
    32521 gcaaccaatg ccaccaggga taaagccccc gcaacattgc gcctcaccgg aacctttccg
    32581 gcttgccgct gattctacga ctgtttttct gttccgaaat ccatcactta ctcactctgt
    32641 gctgcgcatt ccgcagaata ttatccgctt gcaaccgttg catttttctt acgaaaaagg
    32701 ccgcagagtt tcacccctgc ggcttggtcc gggcgcaaat tgccattacg gcagcctgac
    32761 gcccgcatga cacgttactt ccgcttgttc agccagttca cgatcatact ggcaagaatg
    32821 ccagcaatga ccggagccgc taaatcatgc cagaaggcca tgcccagttc tgcgagcgtc
    32881 atatagccgc ctgtgttgta atgacaacgt ttcgcggcta ttcttgagtt gttcaggttc
    32941 aagaatggcc cccgtgatgt tgtcaggtgc atacctgcaa cgtgcggggg ttttctctct
    33001 ccaacaacca atgccaccag ggataaagcc cccgcaacat tgcgcctcac cggaaccctt
    33061 ccggcttgac gctgattcta cgactgtttt tctgttccgg aatccgtcac gtcttcactc
    33121 tgtgctgcgc attccgcaga atattatccg cctgcaaccg ttgcattttt cttacgaaaa
    33181 aggccgcaga gtttcacccc tgcggcctga tccgggcgca aattgccatt acggcagcct
    33241 gacgcccgca tggcacgtta cttccgcttg ttcagccagt tcacgatcat actggcaaga
    33301 ataccagcaa tgaccggagc cgctaaatca tgccagaagg ccatgcccag ttctgcgagc
    33361 gtcatatagc cgcctgtatt gtaatgacaa cgtttcgcgg ctattcttgt attgtttgga
    33421 tgcaagaatg gcccccgtgg tgttgtcagg tgcatacctg caacgtgcgg gggttttctc
    33481 tctccagcaa ccaatgccac cagggattaa gcccccgcaa cattgcgcct caccggaatc
    33541 cttccggctt gacgctgatt ctacgactgt ttttctgttc cggaatccgt ctcatcttca
    33601 ctctgtgctg cgcattccgc agaatattat ccgcttgcaa ccgttgcatt tttcttacga
    33661 aaaaggccgc agagtttcac tcctgcggcc tggtccgggc gcaaattgcc attacggcag
    33721 cctgacgccc acatgacacg ctacttccgc ttgttcagcc agttcacgat catactggca
    33781 agaatgccag caatgaccgg agccgctaaa tcatgccaga aggccatgcc cagctctgcg
    33841 agcgtcatat agccgcctgt gttgtaatga caacgtttcg cggctattct tgagtggtct
    33901 aggttcaaga ttagcccccg ttatgttgtc aggtatatac ctgcaacgtg cgggggtttt
    33961 ctctctccag caaccaatgc caccagggat aaagcccccg caacattgcg cctcaccgga
    34021 acccttccgg cttgccgctg attttacgat tgctttttac tgtcagcacc cggcaatctt
    34081 cctcattgcg cgatgccttc cagcttaatt tctttatttg caatagttgc agttcatatc
    34141 cgggctgact ccagactggt agtgaaatgc gcaattttgt agaattgcgc ctcctcgcga
    34201 cctccccagt gaatctgttg cctcattttt tacatactgc gtattcgact tctccacctg
    34261 ttgcgcaaga gaaactgggt ttattcattt ttgcgagacc gacttctttc tggacaggac
    34321 tttatgcagc acaacacact atcgaaacac aatcagaaat tgccgtttac acgctacgac
    34381 ttcggctggg ttttattatg cataggcatg gcgattggtg ccggaaccgt gctgatgcca
    34441 gtacaaattg gcttgaaggg aatttgggta tttattaccg cagcgatcat tgcttatcct
    34501 gccacctggg tagtgcagga catttattta aaaacccttt ctgaaagcga ttcctgtaat
    34561 gactacaccg atattatcag tcattacctg gggaagaact ggggaatttt cctcggggtt
    34621 atctactttt tgatgattat ccacgggatt tttatctact ctctctccgt ggttttcgac
    34681 agcgcctcgt acctgaaaac cttcggttta accgatgccg atctttcaca atctctactt
    34741 tataaagtcg ctattttcgc cgtactggtg gcgattgcgt ctggtggtga acgattactg
    34801 tttaagattt ccgggccaat ggtggtggtc aaagtaggga ttattgtcgt gttcggtttt
    34861 gcgatgatcc cgcactggaa tttcgccaat ataaccgcct tcccgcaagc ctccgtcttt
    34921 ttccgcgatg tcctgcttac aattccattt tgcttctttt ctgcggtatt tattcaggta
    34981 cttaacccaa tgaatattgc ctatcgtaaa cgggaagcag ataaagtact ggcaacccgg
    35041 ctcgcgctgc gtacccaccg aattagttat atcacgctca tcgcggtgat cctgtttttt
    35101 gccttttcgt ttaccttctc aattagccac gaagaagccg tttctgcctt tgaacaaaat
    35161 atctcggcac tggcgctggc cgcgcaggtg atccctggac atatcattca tatcacctct
    35221 acggtgctga atatctttgc cgtactgacc gcattctttg gcatttatct cggtttccac
    35281 gaggctatta aaggcattat tctcaatctg ttaagccgaa ttattgatac caagaaaatt
    35341 aactcacgca tgctgactct ggcgatctgc gcttttatcg tcattacatt gacgatttgg
    35401 gtttcgtttc gtgtatcggt gctggtgttc tttcagttgg gaagcccgtt atatggcatt
    35461 gtgtcgtgcc tcattccgtt tttcctgatc tataaagtcg cacaactgga aaaacttcgc
    35521 ggatttaaag cctggctaat tctgctttac ggcattttgc tatgcttgtc gccactgttg
    35581 aagctgattg agtaaaccgg agcgcatggc cccggttttg tgagttaacg ctgcggattt
    35641 tcatcctgat caacagcaaa acaagcgacc agttgaccgc cgtagtcttt tagctgcggc
    35701 tgcaactggg tgcaggggcc gaagcgccga cgacagcggg cgttgaaggc gcaacccggc
    35761 ggaggattca gcgggcttgg cagttcaccg gtgagtttga tgcgttcacg acgatcgtcc
    35821 gggttcaggc gtggcgtcgc ggagagcagc gcctgggtgt acggatggcg cgggttattg
    35881 aagatttggt ccttcgttcc tttctccacg cagcggccca ggtacatcac catcacttca
    35941 tcagcaatgt gctccaccac cgacaggtcg tgggagataa agacataaga aagccccaac
    36001 tcctgctgca aatccatcat cagattcagc acctgcgcac gcactgagac gtccagcgca
    36061 gaaaccggtt catcggcaat caccacgtcc gggtcgagca tcagaccacg ggcaatagcg
    36121 atacgctgac gttgtccgcc ggagaacata tgcggatagc ggtcatagtg ctcggttttc
    36181 aggccgactt tcgccatcat cgacagggct ttttcccgac gctgttcttt gctgaagctg
    36241 gtgttaataa gcagcggctc ttcgagaatt tgcccaactt ttttacgcgg attcagcgaa
    36301 ccgtaagggt tctggaagac aatctggatt ttctgccgac gcagcttctg cgcctgcgga
    36361 tcgtgcttga gcagatcctg cccctggtaa tacagctcac caccggtggg cgtttcgatc
    36421 atcgtcagca accgaccgag ggtcgattta ccgcagccag attcgcccac tactgccagc
    36481 gttttgccac gttcaaggtt aaacgaaacg ccgtccagcg ccttcaccag acgttccggc
    36541 gcgaacatgc ctttcttcac cggataatgt tttttcaggt cgatagcctg caacagcggt
    36601 tgttgcgagg tggcctcttg cgtactcata gtgtcggcct cccggcatca tcaagtgggt
    36661 aatggcattt ggactgacgc ccatcagcga gcatattcag cgccggttct tcagcgcgac
    36721 atctgtcagt ggcatagggg cagcgcgggt taagcaggca gccgttcggg cggtcgtact
    36781 taccgggaac gacacctggc aacgacgcca gacgttcttt gtcctgagca aattctggca
    36841 gcgcacgcag caatgcctga gtatacgggt gacgcggcgc atggaagatg gcgtgcgcat
    36901 caccggtttc caccacctgg cctgcataca tcacgatgat tttatgtgcc gcttccgcca
    36961 ccagcgccag gtcatgggta attaacacca gcgccatgtt ctctttctgc tgtagctcca
    37021 gcagtagttc gatgatttgc gcctgaatgg tcacgtccag cgcggtggtc ggttcatcgg
    37081 caatcagcag ttttggccga caggcaatcg ccatggcgat catcacgcgc tggctcatgc
    37141 cgccggaaag ctgatgcggg taaatatcca gacgcgatgc cggatcggga ataccgacct
    37201 gattcagcag gtcaatcgcc cgctgacggc gagtgctctt attaccgccc tgatgcacct
    37261 taatcgcttc catgatctgg aaacccacgg tgtagcacgg gttaaggctg gtcatcgggt
    37321 cctggaagat catcgccact tcggcaccca ccaggttgcg gcgctctttt tctgatatac
    37381 gctgcaaatc ctgaccgtta aacaccagct tttccgccat tacgcggccc ggataatcaa
    37441 tcagccccat aatcgccagt gaactgaccg acttaccgga accagactca cccacaatcc
    37501 cgaccacttc gccctgtttt acgctgtagc tgatgcggtc tacggcgcgg aacggtgcgc
    37561 tttcgtcgcc gaaatgcacc gataatttat ctacatttaa taacgccatc tcgaacctct
    37621 tactgcttca gtttgggatc gagcgcgtca cgcagaccgt cacccatcag gttaaatgcc
    37681 agcaccgtca gcaggatcgc cagacccggg aaggtcacga cccaccaggc gctttgcgcg
    37741 aactgcaaca cgtcggagag catggtgccc cactctggtg ttggcggctg cgctcccatg
    37801 ccgaggaagc caagggcggc catatcgaga atggcgttag agaaaccgag cgacgcctga
    37861 acaatcagcg gcgcaaggca gttcgggaag atgttaataa acatctgacg catcgccccg
    37921 gcacccgcca cgcgagacgc ggtgacgtaa tcgcggttaa cttccaccag cacggcggcg
    37981 cgggttaagc gcacatagtg cggcaaggca acgaaggtca gtgccagcgc ggcgttacca
    38041 atcgacgggc cgaagattgc caccagcacc agcgccagca gcagacttgg cagcgccagc
    38101 atgatatcga ccacgcgcat aatgatgtta tcgaccaggc cgccaaagta accggcgatc
    38161 aggccgagaa taacgcccat aatcagcgat aacacaacta ccagacagcc aaccagcagc
    38221 gacaggcgcg caccgtacat caggcgcgac agcacatcac ggcctacgtc atcggtgccc
    38281 agcaagtgcg ccatgctgcc gccttcctgc caggctggcg gggcgagcag tgcatcgcgg
    38341 aactgttccg ccgggttata gggtgcaatc cagttggcaa agatcgcgat gaacagcacg
    38401 atgacgacgt aaaccagccc gacgaccgcg cctttgttgc gtttaaaata gtgccagaac
    38461 tcctgtaacg gggtcatcgg caccggtgcg ctaatcactt tattttcagt aacctgtgac
    38521 atgatgtccc cttacttctt atgacgaata cgcgggttca ccacgccgta cagcagatcg
    38581 accagcaagt tgacgaggat aatcatcgtc gccaccagca atacgccgcc ctgcactacc
    38641 ggatagtcgc ggcgttgcag cgcgtcaatc aaccagcgcc ccagaccggg ccacgagaag
    38701 atggtttcgg tcagaatcgc ccccgccagc aatgttccca cctgcaggcc gataacggtc
    38761 actaccggca gcatcgcgtt acgcagcgca tggacgataa tcacccgcat gcgggttagc
    38821 cctttggcgc gcgcggtgcg gatgtaatcc tcgcccagca cttccagcat cgaggagcgt
    38881 gtcatacgca caatgaccgc cagcggaata gtacccagca caatggcagg caagatcata
    38941 tgggcgacgg catcgataaa gttgccgtct tcaccccaga tggcggtgtc gattagcata
    39001 aaaccggtta acggattgga gtcatcgagg aacaccatat cgctcacgcg accggagacg
    39061 ggcgtcaggt tccagtgcac cgaaaccagc atgatcagca tcatgcccca ccagaagatc
    39121 ggcatcgagt agccggtcag cgccaggcca accgctgtgt gatcgaaaat ggaaccgcgt
    39181 ttaaccgcag ccagcacgcc gaccggaata ccgaccgcag tcgcaaaaat catcgcgcag
    39241 acgccaagtt ccagcgtggc ctggaggcgc ggcacgaact cttcccaaac cgggatgcgg
    39301 cttttcattg aaatgcctag atcgccatgc ataacgcccc aaatgtaatg gagatactgc
    39361 tgccacatcg gtttatctaa gccgagttca gccagcagct gcgcgtgacg ctctggggag
    39421 atcccacgtt cgcccgccat gatcatcacc ggatcgcccg ggatcatgtg gacaaaggca
    39481 aatgtgagaa gggtaatacc gataaacgtg gggatgacga gtcccaaacg tcggagaata
    39541 aactgcaaca taacccggat tctctatagt gacccgcagc ctggaactgc gtgtctgtat
    39601 tgctcacaaa tcaattcccg cctaggtcgg gctgggaatg aagtattgct gccggatggc
    39661 gctgcacgca gaggcacagc gccaccgggt atggctttta attattcgat agagacgttt
    39721 tcgaagtgat gtttgcctaa tggatcaacc acatagcctt taacttcttt gcgtaccggt
    39781 tcaaacacgg tggagtgagc gatgatcagt gccggagcct gatcgtgcat caccacctgc
    39841 gcttgcttgt acagttcaac gcgtttgtta tggtcgtcgg tagcacgcgc cggttgaatc
    39901 agatcttcaa acggtttgta gcaccatttt gagtagttgg agccttgttc agaggcggcg
    39961 cagctgaaca gggtggcgaa gaagttatcc ggatccccgt tatcgccagt ccagcccatc
    40021 attaccgtct ggtgctcgcc atctttcgca cgcttgaggt actcacccca ttcgtaggtg
    40081 acaattttgg cctgcacgcc gactttcgcc cagtcagcct gaatcatctc cgccatgcgg
    40141 cgagcgttcg ggttatatgg gcgctgtacc ggcatcgccc acaggtcgat ggagaaacct
    40201 ttttccagac ccgcttcttt cagcaaggct ttcgcttttt caggatcgta ggtgtaatcc
    40261 tgaacgtcgt cgttatagcc ccacatggtt ggcgggatca ggtttttcgc tgatacaccc
    40321 gcgccctgat aaaccgcttt gatgatcgcg tctttgttca ccgcgtaggt cagtgcctgg
    40381 cgaactttca cgtcatccag cggttttttc tgcacgttat aggagaggta gccgacgttc
    40441 agccccggca tttccatcag gttgatggat ttatcctgct tcatgcgagc gatgtctgcc
    40501 gggttcgggt acggcatcac ctggcattca ttcttctgca atttcgcata acgcacggaa
    40561 gcgtcaggag taatagagaa gaccagcgta tcgatctgcg gtttggtgcc ccagtagcca
    40621 tcaaacgctt tgtagcggat acgggagtct ttttggtact gctgtaactg gaacggaccg
    40681 gtgccgattg ggttgaggtc cagtttttcc ggtgtaccgg ctttcatcat cgcgtcagca
    40741 tattcttttg acagaataga ggcgaagtcc attgccaggt cagcgaggaa cggcgcttcc
    40801 gggcgagtca gcacaaactg aacggtgttg tcgtccacct ttttcacttc gctgatcagc
    40861 tccggcaagc ccatgccttc gaagtattcg tagctgccgc cagaaacttt atggtacggg
    40921 ttttgcgcgt ttttctgacg atcaaacgag aacaccacgt catcggcgtt cagttcacgc
    40981 gtcggtttga attctttatt gtcgtgccac ttcacacctt tacgcagatg gaaggtatag
    41041 gttttaccgt cttcgctgac ttcccacttt tcagcgaggc ccgggatcac ttcagtggtg
    41101 ccgattttaa attcaaccag gcggttataa agcggtacgg aagaggcgtc ataggtggtg
    41161 ccggaggtaa acagctgcgg gttaaaccct tccggagatc cttctgagca ataaaccaga
    41221 gttttagcct gaacacttgc tgcgacggtc atagccacca ggctgagacc aagcttcagc
    41281 atccctgact ttttcaagga aatacgcatt attctgctcc aattgtgatg tttgttgttt
    41341 taaccctttg cagtgggttg tcgctgcctg accttttttg ttttttgccc ggtcgggtca
    41401 acgttatgag gtggggatgc cgtactaatt aacatcagtg taacagtgcg gatcctccat
    41461 aaaatgcccc tcgtgaccta caatctgtca acagaatgtg aaaacgtcaa tacagccaac
    41521 cgggatttac accaacggtg agaatccaca aacaaagatt aaaaaaactt caaacagcta
    41581 tttgcagcag agaaatttgt gctactccaa catgaccaga acaatcagct taatatttag
    41641 caacatttgg tgattaagtt ttatgcattt aatgaaaaaa atctgaggaa aaggtggata
    41701 tctgagcgat taataccatg aacgctaaaa cgcacagcgg aaaatgccag cgccagccat
    41761 aagtaacgca aaaaaagatt taagcaaata taaaaaaaga caatggtttc tgtcacaaat
    41821 ccgttaatgg atagtgagat atggggcgca aagttggcag gaaaatgtgt ggtgtgttgg
    41881 tcgaataagg cggtcgcgcc gcatccgaca ataagtgcgg tgccgttgcc tgatgcgacg
    41941 ctg
//
LOCUS       DAZGOB010000030        41838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGOB010000030 DAZGOB010000000
VERSION     DAZGOB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.030"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            314..400
                     /gene="leuL"
                     /locus_tag="ACHLIF_001674"
     CDS             314..400
                     /gene="leuL"
                     /locus_tag="ACHLIF_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO9605707.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            493..2064
                     /gene="leuA"
                     /locus_tag="ACHLIF_001675"
     CDS             493..2064
                     /gene="leuA"
                     /locus_tag="ACHLIF_001675"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO9605708.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2064..3155
                     /gene="leuB"
                     /locus_tag="ACHLIF_001676"
     CDS             2064..3155
                     /gene="leuB"
                     /locus_tag="ACHLIF_001676"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO9605709.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3158..4558
                     /gene="leuC"
                     /locus_tag="ACHLIF_001677"
     CDS             3158..4558
                     /gene="leuC"
                     /locus_tag="ACHLIF_001677"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO9605710.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4569..5174
                     /gene="leuD"
                     /locus_tag="ACHLIF_001678"
     CDS             4569..5174
                     /gene="leuD"
                     /locus_tag="ACHLIF_001678"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO9605711.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5223..6401)
                     /gene="setA"
                     /locus_tag="ACHLIF_001679"
     CDS             complement(5223..6401)
                     /gene="setA"
                     /locus_tag="ACHLIF_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO9605712.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6503..6634)
                     /gene="sgrT"
                     /locus_tag="ACHLIF_001680"
     CDS             complement(6503..6634)
                     /gene="sgrT"
                     /locus_tag="ACHLIF_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO9605713.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6723..8378
                     /gene="sgrR"
                     /locus_tag="ACHLIF_001681"
     CDS             6723..8378
                     /gene="sgrR"
                     /locus_tag="ACHLIF_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO9605714.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8412..8511
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8542..9525
                     /gene="thiB"
                     /locus_tag="ACHLIF_001682"
     CDS             8542..9525
                     /gene="thiB"
                     /locus_tag="ACHLIF_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO9605715.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9501..11111
                     /gene="thiP"
                     /locus_tag="ACHLIF_001683"
     CDS             9501..11111
                     /gene="thiP"
                     /locus_tag="ACHLIF_001683"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO9605716.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11095..11793
                     /gene="thiQ"
                     /locus_tag="ACHLIF_001684"
     CDS             11095..11793
                     /gene="thiQ"
                     /locus_tag="ACHLIF_001684"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO9605717.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11907..12671)
                     /gene="yabI"
                     /locus_tag="ACHLIF_001685"
     CDS             complement(11907..12671)
                     /gene="yabI"
                     /locus_tag="ACHLIF_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9605718.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12757..13635)
                     /gene="araC"
                     /locus_tag="ACHLIF_001686"
     CDS             complement(12757..13635)
                     /gene="araC"
                     /locus_tag="ACHLIF_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO9605719.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRYPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13974..15674
                     /gene="araB"
                     /locus_tag="ACHLIF_001687"
     CDS             13974..15674
                     /gene="araB"
                     /locus_tag="ACHLIF_001687"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO9605720.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15685..17187
                     /gene="araA"
                     /locus_tag="ACHLIF_001688"
     CDS             15685..17187
                     /gene="araA"
                     /locus_tag="ACHLIF_001688"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO9605721.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17356..18051
                     /gene="araD"
                     /locus_tag="ACHLIF_001689"
     CDS             17356..18051
                     /gene="araD"
                     /locus_tag="ACHLIF_001689"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO9605722.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18126..20477
                     /gene="polB"
                     /locus_tag="ACHLIF_001690"
     CDS             18126..20477
                     /gene="polB"
                     /locus_tag="ACHLIF_001690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO9605723.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20642..23548
                     /gene="rapA"
                     /locus_tag="ACHLIF_001691"
     CDS             20642..23548
                     /gene="rapA"
                     /locus_tag="ACHLIF_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO9605724.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23560..24219
                     /gene="rluA"
                     /locus_tag="ACHLIF_001692"
     CDS             23560..24219
                     /gene="rluA"
                     /locus_tag="ACHLIF_001692"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO9605725.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYVADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24336..25151)
                     /gene="djlA"
                     /locus_tag="ACHLIF_001693"
     CDS             complement(24336..25151)
                     /gene="djlA"
                     /locus_tag="ACHLIF_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO9605726.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25406..27760
                     /gene="lptD"
                     /locus_tag="ACHLIF_001694"
     CDS             25406..27760
                     /gene="lptD"
                     /locus_tag="ACHLIF_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO9605727.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27813..29099
                     /gene="surA"
                     /locus_tag="ACHLIF_001695"
     CDS             27813..29099
                     /gene="surA"
                     /locus_tag="ACHLIF_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO9605728.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29099..30088
                     /gene="pdxA"
                     /locus_tag="ACHLIF_001696"
     CDS             29099..30088
                     /gene="pdxA"
                     /locus_tag="ACHLIF_001696"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO9605729.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30085..30906
                     /gene="rsmA"
                     /locus_tag="ACHLIF_001697"
     CDS             30085..30906
                     /gene="rsmA"
                     /locus_tag="ACHLIF_001697"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO9605730.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30909..31286
                     /gene="apaG"
                     /locus_tag="ACHLIF_001698"
     CDS             30909..31286
                     /gene="apaG"
                     /locus_tag="ACHLIF_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO9605731.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31293..32141
                     /gene="apaH"
                     /locus_tag="ACHLIF_001699"
     CDS             31293..32141
                     /gene="apaH"
                     /locus_tag="ACHLIF_001699"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO9605732.1"
                     /translation="MATYLIGDVHGCYDELFALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32166..32765
                     /locus_tag="ACHLIF_001700"
     CDS             32166..32765
                     /locus_tag="ACHLIF_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFO9605733.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32801..33268
                     /locus_tag="ACHLIF_001701"
     CDS             32801..33268
                     /locus_tag="ACHLIF_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605734.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(33367..33546)
                     /locus_tag="ACHLIF_001702"
     CDS             complement(33367..33546)
                     /locus_tag="ACHLIF_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO9605735.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33567..34046)
                     /gene="folA"
                     /locus_tag="ACHLIF_001703"
     CDS             complement(33567..34046)
                     /gene="folA"
                     /locus_tag="ACHLIF_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO9605736.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34238..36100)
                     /gene="kefC"
                     /locus_tag="ACHLIF_001704"
     CDS             complement(34238..36100)
                     /gene="kefC"
                     /locus_tag="ACHLIF_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO9605737.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36093..36623)
                     /gene="kefF"
                     /locus_tag="ACHLIF_001705"
     CDS             complement(36093..36623)
                     /gene="kefF"
                     /locus_tag="ACHLIF_001705"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO9605738.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36731..38062)
                     /gene="yaaU"
                     /locus_tag="ACHLIF_001706"
     CDS             complement(36731..38062)
                     /gene="yaaU"
                     /locus_tag="ACHLIF_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9605739.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38121..38408)
                     /gene="fixX"
                     /locus_tag="ACHLIF_001707"
     CDS             complement(38121..38408)
                     /gene="fixX"
                     /locus_tag="ACHLIF_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO9605740.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38405..39691)
                     /gene="fixC"
                     /locus_tag="ACHLIF_001708"
     CDS             complement(38405..39691)
                     /gene="fixC"
                     /locus_tag="ACHLIF_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9605741.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39742..40683)
                     /gene="fixB"
                     /locus_tag="ACHLIF_001709"
     CDS             complement(39742..40683)
                     /gene="fixB"
                     /locus_tag="ACHLIF_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO9605742.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40698..41468)
                     /gene="fixA"
                     /locus_tag="ACHLIF_001710"
     CDS             complement(40698..41468)
                     /gene="fixA"
                     /locus_tag="ACHLIF_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO9605743.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10182 a        11453 c        10885 g         9318 t
ORIGIN      
        1 caatatcaaa aaaaaatcgc aaaacatata attcaataca aatcatcagg ataggttttg
       61 caacgcgtgc attttgtccc ctttttcctc gttgattaga tgcaaaaatt tatgctgaaa
      121 tatgtcaacc gatgaaaagc gtcggtagtt aagcagaaat taatatcgct tactttaacc
      181 accgcagcac aattagctaa ttttacgggt gcagaactca cgctggcggg acgtttttat
      241 tgcgtcaggg ttgacatccg tttttgtatc cagtaactct aaaagcatat cgcattcatc
      301 tggagctgat ttaatgactc acatcgttcg ctttatcggt ctactactac taaacgcatc
      361 ttctttgcgc ggtagacgag tgagcggcat ccagcattaa gccagcacgc agtcaaacaa
      421 aaaacccgcg ccattgcgcg ggttttttta tgcccgaagc gaggcgctct aaaagagaca
      481 aggacccaaa ccatgagcca gcaagtcatt attttcgata ccacattgcg cgacggtgaa
      541 caggcgttac aggcaagctt gagtgtgaaa gaaaaactgc aaattgcgct ggcccttgag
      601 cgtatgggtg ttgacgtgat ggaagtcggt ttccccgtct cttcgccggg cgattttgaa
      661 tcggtgcaaa ccatcgcccg ccaggttaaa aacagccgcg tatgtgcgtt agctcgctgc
      721 gtggaaaaag atatcgacgt ggcggccgaa tccctgaaag tcgccgaagc cttccgtatt
      781 cataccttta ttgctacctc cccaatgcac atcgccacca agctgcgcag caccctggac
      841 gaggtgatcg aacgcgctat ctatatggtg aaacgcgccc gtaattacac cgatgatgtt
      901 gaattttctt gcgaagatgc cgggcgtaca cccattgccg atctggcgcg agtggtcgaa
      961 gcggcgatta atgccggtgc caccaccatc aacattccgg acaccgtggg ctacaccatg
     1021 ccgtttgagt tcgccggaat catcagcggc ctgtatgaac gcgtacctaa catcgacaaa
     1081 gccattattt ccgtacatac ccacgacgat ttaggcctgg cggtcggcaa ctcactggcg
     1141 gcggtacatg ccggtgcacg ccaggtggaa ggtgcaatga acgggatcgg cgagcgtgcc
     1201 ggtaactgtt cgctggaaga agtcatcatg gcgattaaag ttcgtaagga tattctcaac
     1261 gtccacaccg ccattaatca ccaggaaata tggcgcacca gccagttagt tagccagatt
     1321 tgtaatatgc cgatcccggc aaacaaagcc attgttggca gcggcgcatt cgcacactcc
     1381 tccggtatcc accaggatgg cgtgctgaaa aaccgcgaaa actacgaaat catgacacca
     1441 gaatctattg gtctgaacca aatccagctg aatctgacct ctcgttcggg gcgtgcggcg
     1501 gtgaaacatc gcatggacga gatggggtat aaagaaagtg aatataattt agacaatttg
     1561 tacgacgcct tcctgaagct ggcggacaaa aaaggtcagg tgtttgatta cgatctggag
     1621 gcgctggcct tcatcggtaa gcagcaagaa gagccggagc atttccgtct ggattacttc
     1681 agcgtgcagt ctggctctaa cgatatcgcc accgccgccg tcaaactggc ctgtggcgaa
     1741 gaagtcaaag cagaagccgc caacggtaac ggtccggtcg atgccgtcta tcaggcaatt
     1801 aaccgcatca ctgactataa cgtcgaactg gtgaaataca gcctgaccgc caaaggtcac
     1861 ggtaaagatg cgctgggtca ggtggatatt gtcgccaact acaacggtcg ccgcttccac
     1921 ggcgtcggcc tggccaccga tattgtcgag tcctccgcca aagccatggt gcacgtactg
     1981 aacaatatct ggcgtgccgc agaagtcgaa aaagagttgc aacgcaaagc tcaacacaac
     2041 gaaaacaaca aggaaaccgt gtgatgtcga agaattacca tattgccgta ttgccggggg
     2101 acggtattgg tccggaagtg atgacccagg cgctgaaagt gctggatgcc gtgcgcaacc
     2161 gctttgcgat gcgcatcacc accagccatt acgatgtagg cggtgcagcc attgataacc
     2221 acgggcaacc actgccgcct gcgacggttg aaggttgtga gcaagccgat gccgtgctgt
     2281 ttggctcggt aggcggtccg aaatgggaac atttaccacc agaccagcaa ccagaacgcg
     2341 gcgcgctgtt gcctttgcgt aagcacttca aattattcag caacctgcgc ccggcaaaac
     2401 tgtatcaggg gctggaagca ttctgtccgc tgcgtgcaga cattgccgct aacggcttcg
     2461 acatcctgtg cgtgcgcgaa ctgaccggcg gcatctattt cggtcagcca aaaggccgcg
     2521 aaggtagcgg acaatatgaa aaagcgtttg ataccgaggt gtatcaccgt tttgagatcg
     2581 agcgtattgc ccgcatcgcg tttgaatctg ctcgtaaacg tcgccacaaa gtcacctcaa
     2641 tcgacaaagc taacgtgctg caatcctcta ttttatggcg tgaaatcgtc aacgagatcg
     2701 ccacggaata cccggatgtc gaactggcgc atatgtacat cgacaacgcc accatgcagc
     2761 tgattaaaga tccatcacag tttgacgttc tgctgtgctc caacctgttt ggcgacattc
     2821 tgtctgacga gtgcgcaatg atcactggct cgatggggat gttgccttcc gccagcctga
     2881 acgagcaagg ttttggtctg tatgaaccgg caggcggctc agcaccagat atcgcaggca
     2941 aaaacatcgc caacccgatt gcgcaaattc tgtcgctggc actgctgctg cgctacagcc
     3001 tggatgccga tgatgcggct tgcgccattg aacgcgccat taaccgcgca ttagaagaag
     3061 gcattcgcac cggggattta gcccgtggcg ctgccgccgt tagtaccgat gaaatgggcg
     3121 atatcattgc ccgctatgtg gcagaagggg tgtaatcatg gctaaaacgt tatacgaaaa
     3181 attattcgac gctcacgtag tgtacgaagc cgaaaacgaa accccgctgt tatatatcga
     3241 ccgccacctg gtgcatgaag tgacctcacc gcaggcgttc gatggtctgc gcgcccacgg
     3301 tcgcccggta cgtcagccgg gcaaaacctt cgccactatg gatcacaacg tctctaccca
     3361 gacgaaagac attaatgcct gtggtgaaat ggcgcgcatc cagatgcagg agctgattaa
     3421 aaactgcaaa gaatttggcg tcgaactgta tgacctgaat cacccgtatc aggggatcgt
     3481 ccacgtaatg gggccggaac agggcgtcac cttgccgggg atgaccattg tctgcggcga
     3541 ctcgcatacc gccacccacg gcgcgtttgg cgcactggcc tttggtatcg gcacttccga
     3601 agttgaacac gtactggcaa cgcaaaccct gaaacagggc cgcgcaaaaa ccatgaaaat
     3661 tgaagtccag ggcaaagccg cgccgggcat taccgcaaaa gatatcgtgc tggcaattat
     3721 cggtaaaacc ggtagcgcag gcggcaccgg gcatgtggtg gagttttgcg gcgaagcaat
     3781 ccgtgattta agcatggaag gtcgtatgac cctgtgcaat atggcaatcg aaatgggcgc
     3841 aaaagccggt ctggttgcac cggacgaaac cacctttaac tatgtcaaag gccgtttgca
     3901 tgcgccgaaa ggcaaagatt tcgacgacgc cgttgcctac tggaaaaccc tgcaaaccga
     3961 cgaaggcgca actttcgata ccgttgtcac tctgcaagca gaagaaattt caccgcaggt
     4021 cacctggggc accaatccag gccaggtgat ttccgtgaac gacaatattc ccgatccggc
     4081 ttcgtttgcc gatccggttg aacgcgcgtc ggcagaaaaa gcgctggcct atatggggct
     4141 gaaaccgggc attccgctga ccgaagtggc tatcgacaaa gtgtttatcg gttcctgtac
     4201 caactcgcgc attgaagatt tacgcgcggc agcggagatc gccaaagggc gaaaagtcgc
     4261 gccaggcgtg caggcactgg tggttcccgg ctctggcccg gtaaaagccc aggcggaagc
     4321 ggaaggtctg gataaaatct ttattgaagc cggttttgaa tggcgcttgc ctggctgctc
     4381 aatgtgtctg gcgatgaaca acgaccgtct gaatccgggc gaacgttgtg catccaccag
     4441 caaccgtaac tttgaaggcc gccaggggcg cggcgggcgc acgcatctgg tcagcccggc
     4501 aatggctgcc gctgctgctg tgaccggaca tttcgccgac attcgcaaca ttaaataagg
     4561 agcacaccat ggcagagaaa tttatcaaac acacaggcct ggtggttccg ctggatgccg
     4621 ccaatgtcga taccgatgca atcatcccga aacagttttt gcagaaagtg acccgtacgg
     4681 gttttggcgc gcatctgttt aacgactggc gttttctcga tgaaaaaggc caacagccaa
     4741 acccggactt cgtgctgaac ttcccgcagt atcagggcgc ttccattttg ctggcacgag
     4801 aaaacttcgg ctgtggctcc tcgcgtgagc acgcgccctg ggcattgacc gactacggtt
     4861 ttaaagtggt gattgcgccg agttttgctg acatcttcta cggcaatagc tttaacaacc
     4921 agctgctgcc ggtgaaatta agcgatgcag aagtggacga actgtttgcg ctggtgaaag
     4981 ccaatccggg gatccatttc gacgtggatc tggaagcgca agaggtgaaa gcgggagaga
     5041 aaacctatcg ctttaccatc gatgccttcc gccgccactg catgatgaac ggtctggaca
     5101 gtattgggct taccttgcag cacgacgacg ccattgccgc ttatgaagca aaacaacctg
     5161 cgtttatgaa ttaatcccct tgcccggtca aatgaccggg ctttccgcta tcgtccacgt
     5221 catcaaacgt ctttaacctt tgcggttaaa aataatgcga caacagaaat aaccgcaatt
     5281 acccagtaga cagcaaagtg cccccaactt tgtgcaattg ctccctgaat aacgccagcc
     5341 agaattaccc cggtagaaat actgttagta aataaggtgg tagctgcccc cgctcttcca
     5401 ggcattaaat cctgaaacca tagcatccca atacccgcaa caatgccgat aaatacagcg
     5461 ttaaaaagtt gcagcgtcat caacgccata cggctatgaa agaaaatcaa tccggtgtaa
     5521 aacagtactc ctgccgccac tgctatgacc atcattcgcc gcttaccata acgtttgaca
     5581 tagtagccag ccagaatcat tgctggtatt tccagtccag ctgccgtccc catcaggaaa
     5641 cccgccagtt tgtctggcaa tcctaactcg ctactgatcc acaacggcat atcaataatg
     5701 tacatggtgt tgcaggtcca cattaacgtc gaggcgacaa ataacatccg tacgttacta
     5761 tcctgccagc caccttgcat tgataaagca ttttccgacg gcagttctac ccgcgccaca
     5821 gacggaagca taaatgcaat caataccaga ctgagtgtga atatcccggc ggcaatcgaa
     5881 aacatcaccg taaagccgta attcaacgcc agcataaagg ccaacggtgg accgataacc
     5941 catgccagag aaagctgcgc acgcatcacc gagctaaaca tcaccacttc tcgcgccgag
     6001 ttatccgcat attcccgcgc cagagcaaat aactgtggca ttgccgtatt ggccagagat
     6061 gccagaagca caccacaggt gataagcgtc agataatgac gattaaatgc aaacaatagc
     6121 gcattgccga tagccatcaa acagcaaaat ataatcagtt ttcgccgatc gccctgactg
     6181 tcagaacgtt ttgccaacca gaggcttacg ccgatcccag caatagcatt caccgtataa
     6241 aagagtccga tccagaaagg ttgcgcgcca acctcacgac tcagaaataa gctcaatgta
     6301 ggagcctgta gcgccccggc cacccccatc ataaaagcga ccagcataaa tgccgcgtaa
     6361 acaccgttca tacggcgagc catcgtcatt atccagatca tacgttccct ttttagcgcg
     6421 gcgagaataa aaaaaaccag caggtataat ctgctggcgg gtgattttac accaatactc
     6481 agtcacacat gatgcaggca agtcaacttt cagaattgcg gtcatcccac tgcatcagtt
     6541 cttcaagcat ggttaatcgt tgtgggacgc ttaaccaacg caaccagcac aacttcgctg
     6601 tcgcggtaaa atagtgctga taaaactgac gcatggggca cctccttgct tcatcgttag
     6661 gaattattga cttaatatag ggaaaataaa attgctgtct tttgcgcagg agttcccctt
     6721 ttatgccatc tgctcgtctg caacaacagt tcatccgcct gtggcaatgc tgcgagggta
     6781 aatcgcagga cacaacgctc aacgaactgg cagcgttatt gagctgctcg cgtcgtcata
     6841 tgcgtaccct gctcaacacc atgcaggatc gcggctggct gacgtgggaa gcggaagtcg
     6901 ggcgcggtaa acgctcgcgt ctgacattcc tctataccgg gctggcgctt cagcaacagc
     6961 gggcggaaga cctgctggag caggatcgta tcgaccaact agtgcagttg gttggcgaca
     7021 aagcgactgt gcggcaaatg ctggtttctc atctgggccg cagcttccgc caggggcggc
     7081 acatcctgcg cgtgctctac tatcgtccgt tgcgtaatct gctacctggc agcgcattgc
     7141 gccgttccga aacccatatt gcccggcaaa tcttcagttc gctaacgcgc ataaatgagg
     7201 aaaatgggga actggaagca gacatcgccc accactggca gcaaatatca ccgcttcact
     7261 ggcgtttctt tttgcgtcca ggagtccatt ttcatcatgg tcgtgaactg gaaatggacg
     7321 acgtgatcgc ctctttaaaa cgaatcaata cgctgccgct ctattcgcat attgcggaca
     7381 ttgtgtcgcc gacgccctgg acgctggata tccatctcac gcaaccggac cgctggttac
     7441 cgttactgct gggacaagtt ccggcgatga tcctgccgcg cgaatgggaa accctcagta
     7501 actttgccag ccatcccatc ggcaccggtc cgtatgcggt gatccgcaac accaccaatc
     7561 aactgaaaat tcaggcattc gatgacttct tcggttaccg ggcattaatc gatgaagtta
     7621 acgtctgggt tctgccggaa attgccgacg agccagctgg aggtctgatg ctaaaaggtc
     7681 cacagggtga ggaaaaagag attgaaagcc gcctggagga aggttgctac tatttactgt
     7741 tcgacagccg cacccatcgc ggggcgaatc agcaagtcag ggactgggta agctatgtgc
     7801 tttctccaac taatctggtc tatttcgctg aggaacagta ccagcaactg tggttcccgg
     7861 cttatggact gctcccccgt tggcaccatg cccgcaccat aaagagcgaa aaaccggctg
     7921 gcctggaaag cctcatcctg accttttatc aggatcacag tgagcatcgg gtgattgccg
     7981 ggatcatgca gcagattctg gcaagtcacc aggtcacgct ggaaatcaaa gagatcagct
     8041 acgatcagtg gcatgaagga gagatcgaga gcgatatctg gcttaacagc gccaacttta
     8101 cgctgcctct ggatttttcg ctgttcgcgc acttgtgcga ggtgccgctg ctccaacact
     8161 gtattccgat cgactggcaa gccgacgccg cccgctggcg caatggcgaa atgaacctgg
     8221 cgaactggtg ccagcaactg gtcgccagca aagcaatggt gccgcttatc caccactggc
     8281 tgatcattca ggggcaacgc agtatgcgcg gcctgcgcat gaacaccctc ggctggttcg
     8341 attttaaatc agcgtggttt gcgccaccgg atccatgatt gctggtagat aacaaaatca
     8401 ctacactaac gccgttctca acggggtgcc acgcaaacgc gtgcgctgag aaaatacccg
     8461 tcgaacctga tccggataac gccggcgaag ggatttgagg ctccttctca agtcctttgc
     8521 cactcttttt tgaggtgcaa agtgttaaaa aaatgtcttc ccctgctgtt gctgtgcaca
     8581 gcgcccgttt tcgctaaacc cgttctgact gtttatacct acgattcctt cgccgccgac
     8641 tgggggcctg gtccaaagat caaaaaagct tttgaagccg actgtaactg cgaactgaaa
     8701 ctggtggcgc tggaagatgg cgtttcgctt ctcaaccgtc tacggatgga aggcaaaaat
     8761 agtaaagccg atgtggtgct ggggctggat aacaatctgt tagacgccgc cagtaaaacc
     8821 ggactatttg ccaaaagcgg tgtggcagcg gatgccgtta acgttcccgg cggctggaat
     8881 aatgacactt tcgtcccgtt tgattacggc tatttcgcct tcgtctatga caagaacaaa
     8941 ctgaaaaacc cgccgcaaag cctgaaagaa ttggttgaga gcgatcagaa ctggcgggtg
     9001 atttatcagg atccgcgcac cagtacgccg gggctgggcc tgctgttgtg gatgcaaaaa
     9061 gtctatggcg atgacgcccc acaagcctgg caaaaactgg cgaagaaaac cgtcacggtc
     9121 accaaaggct ggagcgaagc ctacggcctg tttttaaaag gtgaaagcga tctggtactg
     9181 agttacacca cctctccggc ttatcacatt ctcgaagaga aaaaagataa ctacgccgcc
     9241 gcgaacttta gcgaaggtca ctatctgcag gtggaagtcg ccgcccgcac cgctgccagc
     9301 aagcagccgg agctggcgca aaaattcctc cagtttatgg tttctccggc ttttcagaat
     9361 gcgatcccaa ccggtaactg gatgtatccg gtggcaaatg tcacgctgcc tgccgggttt
     9421 gaacaattga ccaaaccggc caccacgctg gagttcacac cagctgaagt ggctgcacaa
     9481 cgtcaggcat ggattagcga atggcaacgc gccgtcagcc gttaattccc ggctggttaa
     9541 ttccaggtgt aagcgccgcc acgctggtgg tagcggttgc gctcgcggcg tttctcgccc
     9601 tgtggtggaa cgcgccgcag ggtaactggg tggcagtctg gcaggacagc tacctgtggc
     9661 atgtggtgcg cttctccttc tggcaggcgt ttctctcggc gctactctct gtcgtacccg
     9721 cgatattcct cgcccgcgcc ctctatcgca ggcgctttcc gggtcggctg gcgctgttgc
     9781 gtctgtgcgc aatgaccttg atcctcccgg tgctagttgc tgttttcggc attcttagcg
     9841 tctatggtcg tcagggctgg ctggcatcgc tctgccaatc gctcggtctg gagtggacct
     9901 tttcgcccta cggcctgcaa ggcattttgc tggcgcacgt attttttaat ctgccgatgg
     9961 cgagccgctt attactccag gcactggaaa acattcccgg cgaacaacgt cagcttgccg
    10021 cccagcttgg gatgcgcggc tggcattttt tccgcttcgt cgaatggccg tggctacggc
    10081 gacaaatccc gccggttgcc gcgcttatct ttatgctctg tttcgccagc ttcgccaccg
    10141 tgctatcgct gggcggcggt ccgcaggcaa ctactatcga gctggctatt tatcaggcgc
    10201 tgagttacga ctacgatcct gcccgcgcgg cgatgctggc gctgctccag atggtgtgtt
    10261 gccttgggct ggtgctgttg agtcagcgac taagtaaggc cattgcgccc ggtaccacgc
    10321 tgctgcaagg ctggcgcgat ccggacgatc gtctgcatag ccgcatttgc gacacggtgt
    10381 taatagtgct ggcgctgctg ctgttgctgc caccgttgct ggcggtgatc gtcgatgggg
    10441 taaatcgcca gttgccggaa gtgctggcac aaccggtgct gtggcaggcg ctgtggacct
    10501 cgttgcgtat tgcgctggcg gcaggtgtat tgtgcgtagt gctgaccatg atgctgctat
    10561 ggagcagtcg cgaactgcgg gcgcggcaga aaatgctggc gggtcaggcg ctggagatga
    10621 gcggcatgtt gatcctcgcc atgccgggga ttgtgctggc taccggcttc tttttactgc
    10681 tcaacaacac tatcggcctg ccacaatctg ctgacggcat tgtgattttc accaatgcgt
    10741 taatggcgat cccttatgcg ctgaaagtac tggaaaaccc gatgcgcgat atcaccgccc
    10801 gctacagtat gttgtgtcag tcgctgggca ttgaaggctg gtcgcgctta aaagtggtcg
    10861 agctgcgcgc cctgaaacgt ccactggcgc aggcgctggc ttttgcctgc gtgctgtcga
    10921 ttggtgattt tggcgtagtg gcgttgttcg gtaacgatga tttccgtacc ctgccgtttt
    10981 atctctacca gcaaattggc tcctatcgca gccaggacgg cgcggtcacc gcgttaattc
    11041 tgctactact ctgttttctg ctgtttaccg tgattgaaaa actaccgggg cgaaatgtta
    11101 aaactgactg atatcacctg gctttatcac catttgccga tgcgttttag cttaacggta
    11161 gaacgcggcg agcaggtggc aatcctcggg ccaagcggcg cgggtaaaag taccctgctg
    11221 aatttgattg ccggttttct gacgccagcc agcggttcgc tgactatcga cagcgttgat
    11281 cacaccacga cgccgccgtc acgccgcccg gtatcgatgc tgtttcagga gaacaacctg
    11341 ttcagccacc tgacggtcgc gcagaacatc gggctggggc ttaatccggg attgaagctg
    11401 aacgcggcgc agcaggagaa aatgcacgct atcgcccgcc agatggggat tgataattta
    11461 atggcgcggt taccgggcga gctttccggc ggtcagcgac agcgcgtggc gttagcgcgt
    11521 tgtctggtgc gcgaacagcc gattttattg ctcgatgaac cgttctctgc gctcgatccg
    11581 gcgttacgtc aggagatgct gacgctggtg agcacgagct gccagcagca aaaaatgacg
    11641 ctattgatgg tgtcgcacag tgtggaagat gcggcgcgga tcgccacacg ctcggtggtg
    11701 gtcgccgacg ggcgcatcgc ctggcagggt aagaccaatg agttgttgag cggtaaggcg
    11761 agtgcttcgg cactattggg gattacgggt tagtatgcac tgtcggatgc ggcgtgaacg
    11821 ccttatccga cctacgggta gtgcgttttg taggcctgat aagacgcggt cagcgtcgca
    11881 tcaggcaacc cgcacaagac tatctcctaa cccccaacca ctttacgcaa aatatcgata
    11941 tacaccggca tcaacgggtg gcgaattaac accaccagcg ccaccacgcc gatggcagaa
    12001 attaacggcg tcagccacaa caaacggccg cgcgacaaat aatgacttaa acgatcggtt
    12061 gctttaccgc tgcgccataa ccgccagcac agccagccac caacccacaa aaataccgcc
    12121 gtcgccagca gcaaccattt aaactcgccg ctctgcattc cggctggaat atcgatcgcc
    12181 gcgcccgcca gaatcccagg caggaagtaa aacggtggcc acagcaggca gccgataata
    12241 ttcggcgtaa tgaattttgc caccggcaga tccagcattc ccgccaccat tggcaccagc
    12301 ggacgcgtcg ggccaacaaa gcgaccgacc agaatggtga acatgctgtg ttgatgcaac
    12361 gcatgttcag ttttatcgag taatgctttg tttttcttca gaaatgacca gcgatgcaac
    12421 ggctttttaa aacgccagcc cagccagaag gagatccagt cgcccagcag gcaaccgacg
    12481 atccctgcca gccaggcgtg ccagaaactt aactcgccgc tgccaatcag cgctcccagc
    12541 cccgccatca gcaccgtacc gggtagaatc aaaccgacca gcgccagcga ctccagaaag
    12601 gccaccaaca ccaccgccat caatgaatac acggtagatt gggtaataaa gtgttccagc
    12661 aatgcttgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta
    12721 ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc
    12781 attcactttt tcttcacaac cggctcggaa ctcgctcggg ctggccccgg tgcatttttt
    12841 aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
    12901 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca
    12961 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa
    13021 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
    13081 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
    13141 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
    13201 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
    13261 cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc
    13321 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
    13381 ataacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
    13441 aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
    13501 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
    13561 cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt
    13621 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
    13681 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa cccaaccggt
    13741 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
    13801 taacaaaagt gtctataatc acggcagaaa agtccacatt ggttatttgc acggcgtcac
    13861 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
    13921 atcgcaactc tctactgttt ctccataccc gtttttttgg atggagtgaa acgatggcga
    13981 ttgcaattgg cctcgatttt ggcagtgatt ctgtgcgagc tttggcggtg gactgcgcca
    14041 gcggtgaaga gatcgccacc agcgtagagt ggtatccccg ttggcaaaaa gggcaatttt
    14101 gtgatgcccc gaataaccag ttccgtcatc atccgcgtga ctacattgag tcaatggaag
    14161 cggcactgaa aaccgtgctt gcagagctta gcgtcgaaca gcgcgcagct gtggtcggga
    14221 ttggcgttga cagtaccggc tcgacgcccg caccgattga tgccgacggt aacgtgctgg
    14281 cgctgcgccc ggagtttgcc gaaaacccga acgcgatgtt cgtattgtgg aaagaccaca
    14341 ctgcggttga agaagcggaa gagattaccc gtttgtgcca cgcgccgggc aacgttgact
    14401 actcccgcta cattggcggt atttattcca gcgaatggtt ctgggcaaaa atcctgcatg
    14461 tgactcgcca ggacaccgcc gtggcgcaat ctgccgcatc gtggattgag ctgtgcgact
    14521 gggtgccagc tctgctttcc ggtaccaccc gcccgcagga tattcgtcgc ggacgttgca
    14581 gcgccgggca taagtcgcta tggcacgaaa gctggggtgg cttgccgcca gccagtttct
    14641 ttgatgagct ggacccgatc ctcaatcgcc atttaccttc cccgctgttc actgacacct
    14701 ggactgccga tattccggtg ggcaccttat gcccggaatg ggcgcagcgt ctcggcctgc
    14761 ctgaaagcgt ggtgatttcc ggcggcgcgt ttgactgcca tatgggcgca gttggcgcag
    14821 gcgcacagcc taacgcactg gtaaaagtta tcggtacttc cacctgcgac attctgattg
    14881 ccgacaaaca gagcgttggc gagcgggcag ttaaaggtat ttgcggtcag gttgatggca
    14941 gcgtggtacc tggatttatc ggtctggaag caggccaatc ggcgtttggt gatatctacg
    15001 cctggtttgg tcgcgtactc ggctggccgc tggaacagct tgccgcccag catccggaac
    15061 tgaaagcgca aatcaacgcc agccagaaac aactgcttcc ggcgctgacc gaagcatggg
    15121 ccaaaaatcc gtctctggat cacctgccgg tggtgctcga ctggtttaac ggtcgtcgca
    15181 caccgaacgc taaccaacgc ctgaaagggg tgattaccga tcttaacctc gctaccgacg
    15241 ctccgctgct gttcggcggt ttgattgctg ccaccgcctt tggcgcacgc gcaatcatgg
    15301 agtgctttac cgatcagggg atcgccgtca ataacgtgat ggcgctgggc ggcatcgcgc
    15361 ggaaaaacca ggtcattatg caggcctgct gcgacgtgct gaatcgcccg ctgcaaattg
    15421 ttgcctctga ccagtgctgt gcgctcggtg cggcgatttt tgctgccgtc gccgcgaaag
    15481 tgcacgcaga catcccatca gcccagcaaa aaatggccag tgcggtagag aaaaccctgc
    15541 aaccgcgcag cgaacaggca caacgctttg aacagcttta tcgccgctat cagcaatggg
    15601 cgatgagcgc cgaacaacac tatcttccaa cttccgcccc ggcacaggct gcccaggccg
    15661 ttgcgactct ataaggacac gataatgacg atttttgata attatgaagt gtggtttgtc
    15721 attggcagcc agcatctgta tggcccggaa accctgcgtc aggtcaccca acatgccgag
    15781 cacgttgtta atgcgctgaa tacggaagcg aaactgccct gcaaactagt gttgaaaccg
    15841 ctgggcacca cgccggatga aatcaccgct atttgccgcg acgcgaatta cgacgatcgt
    15901 tgcgctggtc tggtggtgtg gctgcacact ttctccccgg ccaaaatgtg gatcaacggc
    15961 ctgaccatgc tcaacaaacc gttgctgcaa ttccacaccc agtttaacgc ggcgctgccg
    16021 tgggacagta tcgatatgga ctttatgaac ctgaaccaga ctgcacatgg cggtcgcgag
    16081 ttcggcttca ttggcgcgcg tatgcgtcag caacatgccg tggttaccgg tcactggcag
    16141 gataaacaag cccatgagcg tatcggctcc tggatgcgcc aggcggtctc taaacaggat
    16201 acccgtcatc tgaaagtctg ccgttttggc gataacatgc gtgaagtagc ggtcaccgat
    16261 ggtgataaag ttgccgcaca gatcaagttc ggtttctccg tcaatacctg ggcggttggc
    16321 gatctggtgc aggtggtgaa ctccatcagc gacggcgatg ttaacgcgct ggtcgatgag
    16381 tacgaaagct gctacaccat gacgcctgcc acacaaatcc acggcgaaaa acgacagaac
    16441 gtgctggaag cggcgcgtat tgagctgggg atgaagcgtt tcctggaaca aggtggcttc
    16501 cacgcgttca ccaccacctt tgaagatttg cacggtctga aacagcttcc aggtctggcc
    16561 gtacagcgtc tgatgcagca gggttacggc tttgcgggcg aaggcgactg gaaaaccgcc
    16621 gccctgcttc gcatcatgaa ggtgatgtca accggtctgc agggcggcac ctcctttatg
    16681 gaggactaca cctatcactt cgagaaaggt aatgacctgg tgctcggctc ccatatgctg
    16741 gaagtctgcc cgtcgattgc cgtagaagag aaaccgatcc tcgacgttca gcatctcggt
    16801 attggtggta aggacgatcc tgcccgactg atcttcaata cccaaaccgg tccagcgatt
    16861 gtcgccagcc tgattgatct cggcgatcgt taccgtctgc tggttaactg tatcgacacg
    16921 gtgaaaacac cgcactccct gccgaaactg ccggtggcga atgcgctgtg gaaagcgcaa
    16981 ccggatctgc caactgcttc cgaagcgtgg atccttgctg gtggcgcgca ccataccgtc
    17041 ttcagccatg cgctgaacct caacgatatg cgccagttcg ccgagatgca cgacattgaa
    17101 atcacggtga ttgataacga cacccgcctg ccagcgttta aagacgcact gcgctggaac
    17161 gaagtgtatt acggatttcg tcgctaagta gccacatcag gtatgtaacg cctgatgcga
    17221 cgctgacgcg tcttatctgg cctacacgct gcgattttgt aggccggata agcaaagcgc
    17281 atccggcatt gttttccccc tcgccccttt ggggagaggg ccggggtgag gggaaacgac
    17341 ctctcaggag tcaacatgtt agaagatctc aaacgccagg tattagaagc caacctggcg
    17401 ctgccaaaac acaacctggt cacgctcaca tggggcaacg tcagcgccgt tgatcgcgag
    17461 cgcggcgtct ttgtgatcaa accttccggc gtcgattaca gcgtcatgac cgctgacgat
    17521 atggtcgtgg ttagcatcga aaccggtgaa gtggttgaag gtacgaaaaa gccctcctcc
    17581 gacacgccaa ctcaccggct gctctatcag gcattcccct ccattggcgg cattgtgcat
    17641 acgcactcgc gccacgccac catctgggcg caggcgggtc agtcgattcc agcaaccggc
    17701 accacccacg ccgactattt ctacggcacc attccctgca cccgcaaaat gaccgacgca
    17761 gaaatcaacg gcgaatatga gtgggaaacc ggtaacgtca tcgtagaaac ctttgaaaaa
    17821 cagggtatcg atgcagcgca aatgcccggc gttctggtcc attcccacgg cccgtttgca
    17881 tggggcaaaa atgccgaaga tgcggtgcat aacgccatcg tgctggaaga ggtcgcttat
    17941 atggagatat tctgccgtca gttagcgccg cagttaccgg atatgcagca aacgctgctg
    18001 gataaacact atctgcgtaa gcatggcgcg aaggcatatt acgggcagta atgactgtat
    18061 aaaaccacag ccaatcaaac gaaaccaggc tatactcaag cctggttttt tgatggattt
    18121 tcagcgtggc gcaggcaggt tttatcttaa cccgacactg gcgggacacc ccgcaaggga
    18181 cagaagtctc cttctggctg gcgacggaca acgggccgtt gcaggttacg cttgcaccgc
    18241 aagagtccgt ggcgtttatt cccgccgatc aggttccccg cgctcagcat attttgcagg
    18301 gtgaacaagg ctttcgcctg acaccgctgg cgttaaagga ttttcaccgc cagccggtgt
    18361 atggccttta ctgtcgcgcc catcgccaat tgatgaatta cgaaaagcgc ctgcgtgaag
    18421 gtggcgttac cgtctacgag gccgatgtgc gcccgccaga acgctatctg atggagcggt
    18481 ttatcacctc accggtgtgg gtcgagggtg atatgcacaa tggcactatc gttaatgccc
    18541 gtctgaaacc gcatcccgac tatcgtccgc cgctcaagtg ggtttctata gatattgaaa
    18601 ccacccgcca cggtgagctg tactgcatcg gcctggaagg ctgcgggcag cgcatcgttt
    18661 atatgctggg gccggagaat ggcgacgcct cctcgcttga tttcgaactg gaatacgtcg
    18721 ccagccgccc gcagttgctg gaaaaactca acgcctggtt tgccaactac gatcctgatg
    18781 tgatcatcgg ttggaacgtg gtgcagttcg atctgcgaat gctgcaaaaa catgccgagc
    18841 gttaccgtct tccgctgcgt cttgggcgcg ataatagcga gctggagtgg cgcgagcacg
    18901 gctttaaaaa cggcgtcttt tttgcccagg ctaaaggtcg gctaattatc gacggtatcg
    18961 aggcgctgaa atccgcgttc tggaatttct cttcattctc gctggaaact gtcgctcagg
    19021 agctattagg cgaaggaaaa tctatcgata acccgtggga tcgaatggac gaaattgacc
    19081 gccgtttcgc cgaagataaa cctgcgctgg caacttataa cctgaaagat tgcgagctgg
    19141 tgacgcagat cttccacaaa actgaaatca tgccattttt actcgaacga gcaacggtga
    19201 acggcctgcc ggtggatcgg cacggcggtt cggtggcagc gtttggtcat ctctattttc
    19261 cgcgaatgca tcgcgctggt tatgtcgcgc ctaatctagg cgaagtgccg ccgcacgcca
    19321 gccctggcgg ctacgtgatg gattcacggc cagggcttta tgattcagtg ctggtgctgg
    19381 actataaaag cctgtacccg tcgatcatcc gcacctttct gattgatccc gtcgggctgg
    19441 ttgaaggcat ggcgcagcct gatccagagc acagtaccga aggttttctc gatgcctggt
    19501 tctcacggga gaaacattgc ctgccggaga ttgtgactaa catctggcac gggcgcgatg
    19561 aagccaaacg tcagggtaat aaaccacttt cgcaggcgct gaagatcatt atgaatgcct
    19621 tttatggcgt gcttggcacc accgcctgcc gctttttcga tccgcgcctg gcatcgtcga
    19681 tcaccatgcg tggtcatcag atcatgcggc aaaccaaagc attgattgaa gcgcagggct
    19741 acgacgttat ctacggagat accgactcga cttttgtctg gctaaaaggt gcacattcgg
    19801 aagaagaggc ggcgaaaatc ggtcgtgcac tggtgcagca cgttaacgcc tggtgggcgg
    19861 aaacgctgca aaaacaacgg ctgaccagcg cattagaact ggagtatgaa actcatttct
    19921 gccgttttct tatgccaacc attcgcggag ctgataccgg cagtaaaaag cgctacgccg
    19981 ggctgattca ggagggcgac aagcagcgga tggtgtttaa agggctggaa accgtgcgca
    20041 ccgactggac gccgctggcc cagcagtttc agcaggagct gtacctgcgt atcttccgca
    20101 acgagccata tcaggagtac gtccgccaaa ccatcgacaa actgatggcg ggtgaactgg
    20161 atgcgcggct ggtttaccgt aaacgccttc gccgtccgct gagcgaatat cagcgtaatg
    20221 ttccgcctca tgtacgcgcc gctcgccttg ccgatgaaga aaatcaaaag cgcggtcgcc
    20281 ccttgcaata tcagaatcgc ggcaccatta agtacgtatg gaccaccaac ggcccggagc
    20341 cgctggacta ccaacgttca ccactggatt acgaacacta tctgacccgc cagctacaac
    20401 ccgtggcgga gggaatactc ccttttattg aggataattt tgctacactt atgaccgggc
    20461 aacttgggct attttgagca aaaaaaagag ttcgccagat accattttga tgcgtgacga
    20521 atgctttgcc atccagtacc atagcgccct ttccattcct ggacctgaat aacgccacta
    20581 cctcataagc acggtagcgg gtggttattg cctgcaatta aagatataga gccgaacaca
    20641 tatgcctttt acacttggtc aacgctggat cagcgataca gaaagcgaat tgggacttgg
    20701 aaccgttgtc gcggtggatg cgcgaactgt cactttactt ttcccatcta ctggtgaaaa
    20761 ccgtctgtac gcacgcagtg attcccccgt gacccgcgtg atgttcaacc ctggtgatac
    20821 cattaccagc catgacggct ggcagatgca agtcgaagaa gtaaaagaag aaaatggctt
    20881 gctgacctat atcggtactc gcctggatac tgaagagtcc ggcgtagccc tgcgtgaagt
    20941 tttccttgat agcaaactgg tgttcagcaa accgcaggac cgtctgtttg ccgggcagat
    21001 tgaccgtatg gaccgctttg cgctgcgtta tcgcgcgcgt aagtattcca gcgaacagtt
    21061 ccgtatgccg tacagcggcc tgcgcggtca gcgtaccagc ctgatcccgc atcagctcaa
    21121 catcgctcat gatgttggtc gccgccacgc gccgcgcgtt ctgctggctg acgaagtggg
    21181 tttagggaaa accattgaag ccgggatgat cctgcatcag caactgctct ctggcgctgc
    21241 tgaacgtgtg ctgattatcg tcccggaaac cttacagcat cagtggctgg tagaaatgct
    21301 gcgccgtttc aacctgcgtt ttgcgctgtt tgatgatgag cgttatgccg aagctcagca
    21361 cgatgcttac aacccgttcg acaccgaaca gctggtgatt tgctcgctgg attttgcccg
    21421 tcgtagcaaa cagcgcctgg aacatctctg tgaagccgaa tgggacctgc tggtggtcga
    21481 tgaagcgcat cacctggtgt ggagcgaaga cgcgccgagc cgcgaatatc aggctattga
    21541 acaactggca gagcacgtgc cgggcgttct gctgctgacc gcaaccccgg aacagttggg
    21601 aatggaaagc cacttcgccc gtctgcgtct gctggacccg aaccgtttcc acgattttgc
    21661 gcagttcgtt gaagagcaga aaaattatcg tccggttgcg gacgccgttg ccatgctgct
    21721 ggcaggtaac aaactgagca atgacgaact gaacatgctt ggcgagatga tcggcgagca
    21781 ggatatcgag ccgctgttgc aagcagcaaa cagcgacagc gaagatgccc agagcgcccg
    21841 tcaggaactg gtttcgatgc tgatggatcg ccacggcacc agccgcgtgc tgttccgtaa
    21901 cacccgtaac ggtgtgaaag gattcccgaa acgcgagctg cacaccatta agctgccgct
    21961 accgacgcag tatcagacgg ctattaaagt ctccggcatt atgggcgcac gtaaaagtgc
    22021 ggaagatcgt gctcgcgata tgctctaccc ggagcgtatt tatcaggaat ttgaaggtga
    22081 taacgccacc tggtggaact tcgatccgcg cgttgagtgg ctgatgggct acctgaccag
    22141 ccatcgctct cagaaagtgc tggtgatctg cgccaaagct gccactgcgc tgcaactgga
    22201 gcaggtactg cgcgaacgtg aaggtattcg cgctgcggtg ttccacgaag gtatgtcgat
    22261 tatcgaacgt gaccgcgctg ccgcctggtt tgccgaagaa gacaccggcg cacaggtact
    22321 gctgtgctca gaaatcggtt ctgaaggacg taacttccag ttcgccagcc acatggtaat
    22381 gtttgacctg ccattcaacc cggatctgct ggagcagcgt attggtcgtc tggatcgtat
    22441 cggtcaggcg cacgatattc agatccatgt gccttatctg gaaaaaactg ctcagtcggt
    22501 gctggtgcgc tggtatcacg aaggtctgga tgcattcgag cacacctgcc cgaccggacg
    22561 cactatttac gatagcgtct acaacgatct gattaactat ctggcttcac cggatcaaac
    22621 cgaaggcttt gacgatctga tcaaaaactg ccgcgagcaa catgaagcgc tgaaagcaca
    22681 actggaacag gggcgtgatc gcctgctgga aatccactcc aacggtggcg aaaaagccca
    22741 ggcactggca gaaagcattg aagagcagga tgacgatacc aacctgatcg ccttcgccat
    22801 gaacctgttc gatattatcg gtatcaatca ggacgatcgc ggcgacaaca tgatcgtgct
    22861 gaccccgtcc gatcatatgc tggtgccgga cttccctggc ctgtcggaag atggcatcac
    22921 catcaccttt gatcgtgaag tggcgctggc gcgtgaagat gctcagttta ttacctggga
    22981 acatccgctg atccgcaacg gtctggatct gattctttct ggcgataccg gtagcagcac
    23041 gatttcactg ttaaaaaaca aagcgttgcc ggtaggtacg ctgttggtgg aactgattta
    23101 cgtggtcgaa gcccaggcgc cgaagcagtt gcagctcaac cgcttcctgc caccgacgcc
    23161 ggtacgtatg ctgctggata aaaacggcaa caacctggcg gcccaggtgg agtttgaaac
    23221 ctttaaccgc cagcttaacg cggttaaccg tcacaccggc agcaaactgg ttaacgccgt
    23281 gcagcaggat gttcacgcta tccttcagct gggtgaagcg cagatcgaga aatctgcccg
    23341 tgcattgatt gatgccgcac gtaacgaagc cgacgaaaaa ctgtctgccg agctgtctcg
    23401 tctggaagcg ctgcgcgcag tgaacccgaa cattcgtgac gacgaactga ccgccattga
    23461 gagcaaccgt cagcaggtaa tggaaagcct ggatcaggca ggctggcgtc tggatgccct
    23521 gcgtttgatc gttgtaacgc atcagtaacg gagccgaaaa tggggatgga aaactacaat
    23581 ccaccgcagg aaccctggtt ggttatcctg tatcaggatg accacattat ggtggttaac
    23641 aagccgagcg gtttgttgtc tgtgccgggt cgtctggaag agcacaaaga tagcgtgatg
    23701 acgcgcattc agcgtgatta tccgcaggca gaatcggtgc atcgtctgga tatggctacc
    23761 agcggcgtga ttgtagtggc gctgaccaaa gccgcggagc gggagttaaa acgccagttc
    23821 cgcgagcgcg agccgaaaaa gcagtatgtg gcccgcgtct gggggcatcc atcccccgca
    23881 gaaggtctgg tggatctgcc gctgatttgc gactggccaa accgcccgaa acagaaagtc
    23941 tgttacgaaa cgggtaaacc tgcgcagacg gaatatgaag tggtggagta tgtggcggat
    24001 aacacggcaa gagtggtgtt aaaaccgatt accgggcgtt cgcatcaatt gcgtgtgcat
    24061 atgctggcgc tgggtcatcc gattctcggc gatcgttttt atgcatcacc agaagcgaga
    24121 gcgatggcac cacgtttgtt gctacatgca gagatgttga cgattaccca tccggcgtat
    24181 ggcaatagta tgacgtttaa agcgccagcg gatttttaat cgtcacgctg gcgcagttcg
    24241 gtaggcggat aaggcgttca cgccgcatcc ggcaattgtg cgccgattgc ctgatgcgac
    24301 gcgggccgcg tcttatcagg cctacagttt aaaacttatt taaacccttt ctgctgcttt
    24361 atcagctcat atgcctgctg aatttcctgc gctttctgct tcgccatctc catcatctca
    24421 ggcggcaaac ctttcgccac cagcttatcg ggatggtgtt cactcatcag cttacggtag
    24481 gcacgtttga tggtggtcgc atcatccgtc ggcttcacgc ccagcacatt acaggcatct
    24541 tccagcgtcg ggccacgctg cgcttgctgc cagttaccac cgccagattg ctgctgataa
    24601 ccgccgccaa actgtgcacc gccctgcatc atgcgcaaaa actggtcaaa ctgggcgcgg
    24661 gagatcccta attcttcagc aatgacatac agcaccgccc gttcattcgg gtgcagtgaa
    24721 ccatcagcaa acgccgcctg aatctggatc tccagaaaca tacgaattaa gtcaaaacga
    24781 ccaaagcaga cactgcgaaa ctggcgcatc ttttcgcgca gcgggtaatt atctgatttt
    24841 cccactcgga acgcgttttg cgccgcagta cgggaagcgc catgaaggtt cattcggtcc
    24901 atcaactggc tggcgatatg aatatcagcc tccgtgacgc gacctttgga tttggttaaa
    24961 tgccccatca cttcaaaagt ggtggcaaaa aacagcgcct gacgctcacg ctggttggcg
    25021 aaccacgcca ttttacggct acgggcttta tcaaacatat ggccaattaa caggcctaac
    25081 actacgcccc aaaagccgcc gcccatcagt aaggccacgg ccacgccaat gatttttccc
    25141 caatactgca tatattcccc aaatcgacac acggatatca gggctatctc ccacaatata
    25201 aaggtgcttt taccgttttc cggctgcggt caattgtggg acatcgccta taatttgcat
    25261 tatcatacct gtcattcaat gccgtgccta acaccactga cgctattcgg acaggattaa
    25321 cactagcgta gagatgacga gtacgttagt ctctgaccgt ttgtcacgcg caacgttacc
    25381 gatgatggaa caataaaatc aacgtatgaa aaaacgtatc cccactctcc tggccaccat
    25441 gattgccacc gccctttata gtcaacaggg actggcagcc gacctcgcct cacagtgcat
    25501 gttgggcgtg ccaagctatg accgtcctct ggtacagggc gataccaatg acttacccgt
    25561 gactatcaat gctgaccacg cgaaagggga ctacccggat gacgccgtgt ttactggcag
    25621 cgtggatatc atgcagggta acagccgtct gcaggccgac gaagtgcagc tccatcaaaa
    25681 agaggcacca ggacaaccgg agccggtacg taccgttgat gcgctcggta atgtccatta
    25741 cgacgataac caggtgatcc tcaaagggcc gaaaggctgg gcgaatctga acaccaaaga
    25801 taccaacgtc tgggaaggtg attaccagat ggtgggtcgc cagggtcgcg gtaaagcgga
    25861 cctgatgaaa caacgtggcg aaaaccgcta taccattctg gataacggta gctttacctc
    25921 ctgtctgccg ggttctgaca cctggagcgt ggtaggtagc gaaattattc atgaccgcga
    25981 agaacaagtt gcggagatct ggaacgcccg ctttaaggtg ggtccggtac cgatctttta
    26041 tagcccctat ttgcagttgc cggtgggtga caaacgtcgc tctggtttct tgatcccgaa
    26101 cgccaagtac accaccacca actactttga gttctacctg ccatattact ggaacatcgc
    26161 gccaaatatg gatgccacca tcacgccgca ttatatgcat cgtcgtggca acatcatgtg
    26221 ggagaacgaa ttccgctacc tctcccaggc gggcgctggc ttgatggaac tggactatct
    26281 gccttcagat aaagtctatg aagatgaaca cccgaacgat gacagttcac gtcgttggtt
    26341 attctactgg aaccactccg gggtcatgga tcaggtgtgg cgtttcaacg tcgactacac
    26401 caaggtcagc gatcctagct acttcaatga tttcgataac aagtacggtt ccagtactga
    26461 cggctacgca acgcaaaaat tcagcgttgg ctatgcggtg caaaacttca atgccaccgt
    26521 ttcaaccaag cagttccagg ttttcagcga acagaacacc agtagctact cggcagagcc
    26581 gcagttagac gttaattact accagaatga tgttggtcca tttgatacgc gtatttacgg
    26641 ccaggcagtg cactttgtta acaccagaga cgacatgcct gaagcaaccc gtgttcacct
    26701 ggaaccgacc atcaatttgc cgctctctaa taactggggc agcatcaata ccgaagcgaa
    26761 gttgctggca acccattatc agcaaaccaa tcttgactgg tataactcca gaaacacgac
    26821 caagctggac gaatccgtta accgcgtaat gccgcaattc aaagttgacg gcaaaatggt
    26881 ctttgaacgc gatatggaaa tgctggctcc gggttatacc caaacgctgg aaccgcgcgc
    26941 gcagtatttg tacgtgccgt atcgcgatca gagcgacatc tataactacg actcgtctct
    27001 gctgcaatct gactactctg gcctgttccg ggaccggact tacggcggtc ttgaccgtat
    27061 tgcctccgct aaccaggtga cgaccggtgt cacatctcgc atatatgatg atgctgccgt
    27121 tgaacgtttt aatatttccg ttggtcaaat ctactatttc acggagtctc gcactggcga
    27181 tgacaacata acatgggaga atgacgacaa aacgggttca ctggtgtggg caggcgatac
    27241 ttactggcgt atctccgagc gttggggatt gcgtggcggg attcagtacg atacacgtct
    27301 ggataacgta gcgaccagta actccagcat tgaataccgt cgggatgaag accgtctggt
    27361 acagctgaat taccgttacg ccagcccgga atatattcag gctacgctgc ctaagtacta
    27421 ttccactgct gagcaatata agaatggtat ttcgcaggta ggtgctgtcg ccagctggcc
    27481 aattgccgat cgttggtcca ttgttggggc ctactactac gacaccaatg cgaacaagca
    27541 agccgactct atgttaggtg tgcaatacag ctcctgctgc tatgcaattc gcgtcggtta
    27601 cgagcggaag ctgaacggtt gggataacga taaacaacat gcggtatatg acaacgcaat
    27661 cggctttaac atcgaacttc gcggcctgag ctccaactac ggtctgggta cgcaagagat
    27721 gctgcgttcg aacattctgc cgtatcaaaa cactttgtga tttgttgatt taccacgtaa
    27781 tccgcagtgc ggttaattga aatggaaaaa gtatgaagaa ctggaaaacg ctgcttctcg
    27841 gtatcgccat gatcgcgaat accagtttcg ctgcccccca ggtagtcgat aaagtcgcag
    27901 ccgtcgtcaa taacggcgtc gtgctggaaa gcgacgttga tggattaatg cagtcggtaa
    27961 aactgaacgc tgctcaggca aggcagcaac ttcctgatga cgcgacgctg cgccaccaaa
    28021 tcatggaacg tttgatcatg gatcaaatca tcctgcagat ggggcagaaa atgggagtga
    28081 aaatctccga tgagcagctg gatcaggcga ttgctaacat cgcgaaacag aacaacatga
    28141 cgctggatca gatgcgcagc cgtctggctt acgatggact gaactacaac acctatcgta
    28201 accagatccg caaagagatg attatctctg aagtgcgtaa caacgaggtg cgtcgtcgca
    28261 tcaccatcct gccgcaggaa gtcgaatccc tggcgcagca ggtgggtaac caaaacgacg
    28321 ccagcactga gctgaacctg agccacatcc tgatcccgct gccggaaaac ccgacctctg
    28381 atcaggtgaa cgaagcggaa agccaggcgc gcgccattgt cgatcaggcg cgtaacggcg
    28441 ctgatttcgg taagctggcg attgctcatt ctgccgacca gcaggcgctg aacggcggcc
    28501 agatgggctg gggccgtatt caggagttgc ccgggatctt cgcccaggca ttaagcaccg
    28561 cgaagaaagg cgacattgtt ggcccgattc gttccggcgt tggcttccat attctgaaag
    28621 ttaacgacct gcgcggcgaa agcaaaaata tctcggtgac cgaagttcat gctcgccata
    28681 ttctgctgaa accgtcgccg atcatgactg acgaacaggc ccgtgtgaaa ctggaacaga
    28741 ttgctgctga tatcaagagt ggtaaaacga cttttgctgc cgcagcgaaa gagttctctc
    28801 aggatccagg ctctgctaac cagggcggtg atctcggctg ggctacacca gatattttcg
    28861 atccggcctt ccgtgacgcc ctgactcgcc tgaacaaagg tcaaatgagt gcaccggttc
    28921 actcttcatt cggctggcat ttaatcgaac tgctggatac ccgtaatgtc gataaaaccg
    28981 acgctgcgca gaaagatcgt gcataccgca tgctgatgaa ccgtaagttc tcggaagaag
    29041 cagcaagctg gatgcaggaa caacgtgcca gcgcctacgt taaaatcctg agcaactaat
    29101 ggttaaaacc caacgtgttg tgatcactcc cggcgagccc gccgggattg gcccggactt
    29161 agttgtccag cttgcacagc gtgagtggcc ggtcgaactg gttgtttgtg ccgatgccac
    29221 tctccttacc gaccgggcag cgatgctcgg tttgccgctc accctccgcc cttattcccc
    29281 caactcccct gcacaaccgc aaactacggg cacattaacg ctacttcctg tcgcgctacg
    29341 tgaatctgtc actgcggggc agttagcgat tgaaaatgga cattacgtgg tggagacgct
    29401 ggcgcgagcg tgtgatggct gtctgaacgg tgaatttgct gcgctgatca caggccccgt
    29461 gcataaaggc gtcattaacg acgcaggcat tccgtttacc ggtcataccg agtttttcga
    29521 agagcgttcg caggcgaaaa aagtggtgat gatgctggcg accgaagaac ttcgcgtggc
    29581 gctggcaacg acgcatttac cgctgcgcga tatcgcagat gctatcaccc ctgcactttt
    29641 gcacgaagtg attgctattt tgcatcacga tttgcgtaca aaatttggta ttgccgaacc
    29701 gcgcattctg gtctgcgggc tgaatccgca cgcgggcgaa ggcggtcata tgggtacgga
    29761 agagatagac accattattc cggtgctcga cgagctgcgg gcgcagggga tgaaactcaa
    29821 cgggccgctg cctgccgata ccctgtttca gccgaaatat ctcgataacg ccgacgccgt
    29881 gctggcgatg taccacgatc agggtcttcc cgtgctaaaa taccagggct tcgggcgcgg
    29941 tgtgaacatt acgctgggcc tgccctttat tcgcacatca gtggaccacg gcaccgcgct
    30001 tgaactggcg ggacgtggcg aagccgatgt cggcagtttt attacggcgc ttaatctcgc
    30061 catcaaaatg attgttaaca cccaatgaat aatcgagtcc accagggcca cttagcccgt
    30121 aaacgcttcg ggcaaaactt tctcaacgat cagttcgtga tcgacagcat tgtttctgcc
    30181 attaacccgc aaaagggcca ggcaatggtc gaaatcggcc ccggtctggc ggcattgacc
    30241 gagccggtcg gcgaacgtct ggaccagctg acggtcatcg aacttgaccg cgatctggcg
    30301 gcacgtctgc aaacgcatcc attcttaggc ccgaaactga cgatttatca gcaggatgcg
    30361 atgaccttta actttggcga actggccgag aaaatgggtc agccgctgcg tgttttcggc
    30421 aacctgcctt ataacatctc cacgccgttg atgttccatc tgtttagcta tactgatgcc
    30481 attgccgaca tgcactttat gttgcaaaaa gaggtggtga atcgtctggt tgcaggaccg
    30541 aacagcaaag cgtatggtcg attaagcgtc atggcgcaat actattgcaa cgtgatcccg
    30601 gtactggaag ttccgccgtc agcctttaca ccaccaccca aagtggattc cgccgtcgtg
    30661 cgcctggttc ctcatgcaac gatgcctcac ccggttaaag atgttcgtgt gttgagccgc
    30721 atcaccaccg aagcctttaa ccagcgtcgt aaaaccattc gtaacagcct cggcaacctg
    30781 tttagcgtcg aggtgttaac gggaatgggg atcgacccgg cgatgcgagc ggaaaatatc
    30841 tctgtcgcgc aatattgcca gatggcgaac tatctggcgg agaacgcgcc tttgcaggag
    30901 agttaacgat gatcaattcg ccccgagtgt gtattcaggt tcaaagcgtc tacattgagg
    30961 cacaatcttc acctgataat gaacgttacg tttttgctta taccgtaacc atacgcaatc
    31021 tggggcgagc gccagtgcag ttgttggggc gttactggct gatcaccaat ggcaatggcc
    31081 gtgaaaccga agtccagggc gaaggagtgg ttggcgtcca gccacttatc gcgcctggcg
    31141 aagagtacca gtacaccagc ggtgcaatca ttgaaacccc gctgggcacc atgcagggtc
    31201 actacgaaat gatcgatgaa aatggcgtcc ctttcagcat cgacattccc gtattccgac
    31261 tcgccgttcc cacactcatt cattaaaaga atatggcgac ataccttatt ggcgacgttc
    31321 atggttgtta cgatgaactg ttcgcattgc tgcataaagt agaatttacc cctgggaaag
    31381 ataccctctg gctgacgggc gatctggtcg cgcgcgggcc gggttcgctg gatgttctgc
    31441 gctatgtgaa atccttaggc gacagcgtac gtctggtgct gggcaatcac gatctgcatc
    31501 tgctggcggt atttgccggg atcagccgca ataaaccgaa agatcgcctg acaccgctgc
    31561 tggaagcgcc ggatgccgac gagctgctta actggctgcg tcgccagcct ttgctgcaaa
    31621 tcgacgaaga gaaaaagttg gtgatggccc acgcagggat cacgccgcag tgggatctac
    31681 agaccgccaa agagtgcgcg cgcgatgtag aagcggtgct atcgagtgac tcctatccct
    31741 tctttctcga cgctatgtac ggcgatatgc caaataactg gtcaccggaa ttgcgggggc
    31801 tgggaagact gcgttttatc accaacgcct ttacccgtat gcgtttttgc tttccaaacg
    31861 gtcaactgga tatgtacagc aaagaatcgc cggaagaagc ccctgcccca ctgaaaccgt
    31921 ggtttgcgat tcctggccct gtcgctgaag aatacagcat cgcctttggt cactgggcat
    31981 cgctggaagg caaaggtacg ccggaaggta tttacgcgct ggataccggc tgctgctggg
    32041 gtgggtcatt aacctgcctg cgctgggaag ataagcagta ttttgtccag ccgtcgaacc
    32101 ggcataagga tttgggtgag ggagaggcgg tcgcgtctta acgaattacc attcagggag
    32161 gaataatggc tgactatttt gaaatacgtg ctgattacaa tcagcacacc atcacgattt
    32221 atcaggctta caatgatgca atcgctgacg tcgccgtacg tgacgggcga tttggcgcac
    32281 ctttttcatt caaccgcatg acctggatta aaccctcttt tatgtggatg atggagcgta
    32341 gcaactgggg actgaaaaaa gatcaacaac acattctcgc aatccgaatc aaacgcacct
    32401 tctttgatac attgctggaa caagcggtgc taaccacgcc ggaagcccat gtgtatccac
    32461 atgcaggaat ttgggaaacg ttatttgcac aagcaaatgt gtatgttcag tgggacccag
    32521 aacgcagcat aaatggtaaa aaactggagc accgatcatt acagcttggt atttctcgta
    32581 acttgatcag tcaatttaac gaagacgcga ttgttgctat tgatgatctg acgccgctgg
    32641 tgcgaaagtg tcataacctt ttgataaacg gcaaaacaac acaggcgaaa tccttcctgc
    32701 cgccagaaaa aatttaccca gttagtgcag cagcgagaaa ggctttggga atgaaaaatt
    32761 cctgagacaa atacgcacct attaagcgga aaatatctaa gtgcgacaaa tcaacaccgg
    32821 gcttaatacc agagaaatta ttctggaaat gattgacgaa gcagatgccc ccccgtttgc
    32881 tgagcagcgg acctggcatc gcaggcgaat cgcttttcta tgtgattgca tcagcgttgc
    32941 tgtcgactac ggcttcttct gttctgtctc tcagcagtca ataatgaaca taaccaagat
    33001 ggctgaagat gtatggataa cagaaagtaa aacgacccaa ctcattgata tttggcaaca
    33061 acttaaacag ctttatccgc cagaaactat tccctacgcc cagaagttac ccactaagga
    33121 attgcttgaa ctgctacttt ctagtcctga cgatcttcga ataattgaaa ttcctgacat
    33181 catcggtgat gtttttgatg accaaacctg gattcgatta ttcaaaaaac actttgctga
    33241 tgttattcag ctacgatgtg atgtgtaaca atccaaaata tatcgccaga cgatagacca
    33301 aaaagaaagt atttagcaga tactcaacat tctgacttct gtacagaaat agcagagttt
    33361 aaaaacttaa aacttccggt actcatcaga gaaacatcca gtttcttctg caaattcatt
    33421 taatgccgag atggcatcct cgtttacttc attccactgt agagtggcct gaaggtttag
    33481 acctacgcaa tgagcgcgac tcacaaaatc gcgctcactg gtaaaatcaa acgtcttatc
    33541 gttcaatttg tagtcctggc aaaaaattac cgccgctcca gaatctcaaa gcaatagctg
    33601 tgagagttct gcgcatcagc atcgtggaat tcgctgaata ccgattccca gtcatccggc
    33661 tcgtaatccg ggaaatgggt gtcgccttcc acttctgcgt cgatatgcgt cagatacagt
    33721 ttctgcgctt ttggcaggaa ctgctcataa acgcgaccgc cgccaatcac catgatttct
    33781 ggtacgtcac cacacgccgc gatggcttca tccaccgact tcacccacgt tacgcgatcg
    33841 tccgtacccg gttgactgct gaggataata tttttgcgtc ctggcaacgg acgaccgatt
    33901 gattcccagg tatggcggcc cataatcacg ggtttattta aggtgttgcg tttaaaccag
    33961 gcgagatcgg caggcaggtt ccacggcatg gcgttttcca tgccgataac gcgatctacc
    34021 gctaacgccg caatcagact gatcattgag atttcccgat aaaaaaaatt gtcgccacta
    34081 tacgtaaagc gtaaaccgtc gtcgactggt gcgaggatga tgttgaggaa aattttatat
    34141 tctgctggcg agtccacgct ctctccctgg actcgccgca ttacaatgaa acaaaaacaa
    34201 acagttagct gtaaagtgtg atttacgtca ctctttatta ggatgagggt ttcgtttccg
    34261 gttcatccgc catgttgccg gtatgtttac cttcttcggt tccctgccag ccatgtcgtt
    34321 gaattaatga cagatgttcg cggtcctcgg taatgatctc acttaacatc gcgctggtgc
    34381 gtttatagac cgccgcgcgg gctttggtgt cgttctcaac cattgccatc tcttccacca
    34441 tctgaatatt aaagcggcgg aacacatcgg cacgttctcg cgcttcatac ggccccagac
    34501 ctaaactttc cagtgccaga cgcccggttt tcagcgcacc ttcgaaggtt tcacgctccg
    34561 gcttttcaac gcctgcctga cgcaaacgaa tgtagtggtc gacatcgcgg gcgcgggcaa
    34621 taatctgcaa atgcgggaaa tgttctttca ccatctccgt cagttgcagg ttggtttgcg
    34681 gatcatcaat agcattaatc agcacttccg ctttcgccgc tccggcagat tccagtaaat
    34741 ccatccgcgt tgcatcgcca taaaacactt tcataccaaa tttacgcagc gtttcgatat
    34801 ggtccggatc gtgatcgagc accaccattt tcaccccgct ggagagcagt aaacgtccgg
    34861 taatctgccc aaaacgaccg aatccggcga taatcacgcg cggctgttct tcgtcgatct
    34921 catcggcttc acgcgcttcc tcagtagaag attgctcaag gcgattgagg atcaccagca
    34981 gaatcggcgt tgctgccatc gacagcgcca ccgccagggt cagcgatttc gcccactccg
    35041 gctccagcac attcgccatc tgcgccgcgc caaataccac aaaggcaaac tcactgccct
    35101 gccctaacaa caccgcaaac caacgacgct gtttatttgg cacttgcaac ggtcgggcaa
    35161 tcagccacag catggcgatt ttgatgatga ggaaaccgag cagcaaaatg acaatgcgca
    35221 atgggttttc aagcagcgtg ccaaagtcta tcgacatgcc aacaccgatg aaaaacagcc
    35281 ccaacagcaa acctttaaat ggttcgatat cgctctccag cgcatgacgg tattccgagc
    35341 ttgccagcag tacgcccgcc agaaacgcgc ccatcgccat cgacaagccg acctcttcca
    35401 gcagcaaacc aaagccaaac acgaggaata acgccacggc actaaacact tcccgcaagc
    35461 cagagcgggc tacaaaacgc agcgccggac gcgtgacata gcgccccagc aataccacca
    35521 gcaccagcgc acccgccact tttaacgccg agagagcaaa tgcgcccatc gtcgtcgagg
    35581 cactgctcgt tgccagtagc ggaatcatcg ccaccagcgg gatcgccgcg atatcctgga
    35641 acagcagcac cgcaaaggca ctgcgaccca tttgcgtcac catcagattg cgttcattca
    35701 tcgcctgcat ggcaatcgcc gtagaggaga gcgccagcgt catgccgatc aattccgcga
    35761 cctgccagcg cagcccaagt aacatgcaga acagccccag caggccgccg caaatcacca
    35821 tctgcaatgc gccaccgccg aacactgccg cacgcagctt ccacagcctt tgtggatcga
    35881 gttcgaggcc gataataaac agcatcagca ccaccccaat ctcggcaaag tgcagaatag
    35941 attcggcatc ggtcaccagt cgcagccccc acgggccaat aatgcagccg gcgatcaggt
    36001 agccaagtac cgatcccaga ccaagacgta ccgcaatggg tacaatcagc gctgccgaac
    36061 cgagataaat cagcgcctga atcagcgtat ggctatccat gatgggcctc ctgccattcc
    36121 agcagacgtt gcttatagtg acgcgcctgc ccttcgaggg tttcgtcgtc acaaataaag
    36181 gtgcagtgca tggcaaacgg tggcagccag ttcagtccgc agtagattgc cgtcgcctgt
    36241 agcggctgcg acagcacatc aaagcccgga tgcgcaccaa tttcaaaatg gctttccccg
    36301 ccgccggtcg tcaccgccca cagcaaatgt ttgccatgca gcgccgtgcc gccatgaccg
    36361 taagcccagc cgtgggagaa aactttatcg atccaaagtt tgaggagcgg aggaatgctg
    36421 taccactgca tcgggtgctg ccagacgatc agatcggcgc gagacagcgc ctcctgctcg
    36481 gcggcaatat cgatattgaa gtcaggatag agttgataaa gagagcgaat ttcgacgcct
    36541 tccagcgtcc ttgcctgttc aagcatccgt ttattcgcat gggaatgatg cggatacgga
    36601 tgcgcataaa ttataagaat cataggtagc ccgcgtgatt acgcctttgt tttcccacaa
    36661 agtgtagtca tatattcacg cggcttatag tcaataatat tgattcagaa aagagagaaa
    36721 tctgaacaat ttaccccatt ctctgcccgc ggatcgtcat attgctggtt tgcgccagtg
    36781 acatccctcg agtctccggg gcaaacgcta cggaaatcaa caagccaaac agcgagatac
    36841 ccgcccccat tagcatcgtg ttactgatac cgtaattatt gataaagatc ggcagtgccc
    36901 aggtcgaaac aatggtgcca atacggctta aggacataat cacgcccacg gcagaggcgc
    36961 ggatatctgt cgggaagagt tcattaggat agagccactg caaattaccc ggcccgccag
    37021 agaaaaaggc atacaccgca aaggccatca ctaccagcca gatccccata tccgggatca
    37081 gccccaaaac cgccagcgcc agcgtcatca tggcaaagct gccaatcaac aatggacgcc
    37141 gtccggcagt gtttaaccac agcatcggcg gaatacagcc gagcataaag aacaggctaa
    37201 tcaccacgtt ccccagcgcc gcgtttttgc caacacccaa tcccaacaaa ccaacgattt
    37261 gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag atggcggcaa
    37321 caaacagaac aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg
    37381 gctgctcttc atcgaaagcc accggttcgc caaacagttt tatcatcatc tcttcgcact
    37441 cttttactcg ccctttgcgt aataaccagc ggggagattc aggcagttcg aatcgaccaa
    37501 tcaaaatcaa caaacagggg atcgccgcgc tacccagcat ccagcgccag ccgccttcca
    37561 catcataaag ccagtagccg accagatcgg cgcaggtcgc gccgacatac cacatggcgg
    37621 cgataaagct gatggaaaac gcccgctgac gggtactgga gaactcggtg atcattgagg
    37681 tggcgatggg ataatctgca ccgatgacaa tgccgataag tacccgcatc accaacagtt
    37741 cgacggggga tgaaacaaac atcgtcgcca ccgatatcac gccgatggcg atgatatcaa
    37801 tgaggaacat tttgcgccgt ccgactttat cggaaatata gccaaacagc gatgtgccaa
    37861 cgaacagccc ggcgagcgtt cccgcgccca acaagccaat ccagtcagcg tccagtttta
    37921 gcgccggagt cagttgctcc agcgccacgc caatcattac cagtacataa ccatccagaa
    37981 acggaccacc gcttccccac agcaaaatgc ggcggtgaat tgaggagaat ttgagatcgt
    38041 caaagtttct ggacggttgc atgggttatt cctgtttttt tgcaatgaga gtcagtacgg
    38101 cgttacgtat caaaccaaca tcagccgtaa cggaactcca caccaaaggt gccgcgcggg
    38161 tattcccact gttccagcgc cgaccccagc cccagaatgc gacaggtgcc gcactccaga
    38221 catccggcgt aatcgaagcg cacactgccg tcatcctgct tcttgtacag acctgcgggg
    38281 cacgctttca ccagcagctc cagggcctgt ttatcggcat cggccttcac aacaatgtgc
    38341 ggatgctctt catcgacatt gaatttattg acgcccagtt tgacgtccac attgacggga
    38401 gaagtcataa aacggtcact cctttcatgc catccttgat cagattgatg aagcccactt
    38461 tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg cgcactgccg tcaatggtga
    38521 acaggtcacg cgccacgccc accgccagtt ccgggtagcc gctaaacatg cgtgggttat
    38581 caaggaaggc cggtagtttc tggtacatac gcatatcgcg cagcgggcca ctctcaagat
    38641 gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc gcttttcatc gctgaaagca
    38701 cggtttttgc tgcagcttcc ccggcggcaa tcgccagatc cataccgcga atggtaaaac
    38761 cgaggttcat acacattccg gcggcatcac cggcaatcaa tacgccgtca ccaaccaact
    38821 ccggcagcat gttgatgcct gcttccggca ctacgtgagc ggaatattcc accagcttgc
    38881 cgcccgcgat cagcggtgcc actgccggat gctgtttgaa atcttccagc atttgcggca
    38941 ccgatttttt tgcgtcatgc agatgatgca gaccacaaac cagccccagc gacagggtat
    39001 tttcattggt ataaaggaag ccgccgccca tcaggccatc ggtgggtgat cccgcaaaca
    39061 gacaagccgc gccctgatta ccctgcaact gaaaacggtc ttcgattacc gacttcggta
    39121 actcgatcag ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt tttgccatcc
    39181 ccagcttttc ggcaaggatg gagttcactc catcagcaag gatcaccgtt ttcgcttcaa
    39241 tcacatcgcc atcggcttct acaccgacga ctttgccatc gcgctgtacg aggttatcga
    39301 cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc agccaggcat
    39361 caaatttact gcgcaaaacg gagtaagaac gctgggatgg cgaggtttcg tcaccattgc
    39421 agtagtccat agtcatcgct gacttttccg tcataaacgc gagtttttca tgggtgatca
    39481 ggcgttctac gggggcggag tcggcgaaac caggaataat gtgttccagg ctgtgggcat
    39541 agagacgccc gccggtgacg ttcttggcac ctgcggaatt gccacgctcg ataactaaca
    39601 cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga accggcaagc ccagcaccga
    39661 cgatgatggc gtcaaagata tcttcggaca taagagctcc ctgtcagcgg cagggccaaa
    39721 atgcagccct gccagagtgg atcaacgcgc taaagctgcg gtcagcgccg gaaggatctt
    39781 cacggcgtca ccaacaatgc cgtaatccgc gtactggaag atcggcgcat ttttatcttt
    39841 attgatggcg aaaatggttt gcgatgcatt agcaccaacc atgtgctgga tctgcccgga
    39901 gatccccacc gccaggtaca gttcaggttt cagcatcagg ttggagatac cgacatagcg
    39961 ttcgtgctcc atccattttt cgttttccgc caccggacga gaacaggcca actccgcacc
    40021 tatcgccttg caaagctgtt ctgccagcgc aatgttctct ttgctgccaa taccgcgacc
    40081 gacgctgacc accagacggg ctttgtcgag atcgacgctg ttgctctggc gcgcctgggt
    40141 tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc acggtgtgcg tttcgccagt
    40201 gcgtgacgcg tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca gtaccgcata
    40261 cggcgtggca atgcgttctt cgccaatcgc cagaccaccg taaaccatgt gtttcactgt
    40321 cgctttaccg tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg ctttaaggcg
    40381 ataacccagt tttgccgcca gtaatttgcc gcgacgggtg tttggcagca gcaccaggcc
    40441 gtctgcgccg tgctggcgaa tagtgtcagc catgacaccg gcgtaatctt cgatcatccg
    40501 atcgtccggt ttgccgttta atttccagac atgattagct ccgagctgga ttgcctgtac
    40561 gccgtcggca tcattgagga caaaggtgtt gatttgatta gctaaagcct gcgcaccgtt
    40621 catcagttcc ggcagacgag aaggggtatc gctgaatacc cagacttgag aaaacgtgtt
    40681 catagcatcc cctgtaatta aatgactttg cgaagatttt cagcaaatgc ggcgatctgt
    40741 tcttcgccgt cgccttcaat cacgatgcgc tgacgttcgc gctgtttcgg cgcagcaacc
    40801 tgttgttcag accaggcgtc cacggcgtta aaaccgatat ccgccgccga ccatacctgg
    40861 actggctttt tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg ggagttgata
    40921 tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta aggtttcggt ttcatcttcc
    40981 agttcgcgct caacggtgag ggtatctgca gtcagggaga taattttgct gacgccgtta
    41041 actgccggaa tattgaggat ttcgcccacc agcagaccaa cctgctgggc ataaaggtcg
    41101 gaagaaccat cgccacagag gatcaaatca aagcctgctt tctgggcggc tgcagccagt
    41161 gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt catcaatcac cacaatcagt
    41221 tcatccgggc cgcgcgatag cacatcttta cgccctttgg cgttggtcag ggctttaccg
    41281 cccacactta aggccgtcac ctgcgcctct gctgcctgtt gctttagctg gcaagccgct
    41341 tcaatagcgt tcagatcgta ttggcttatt ttggcatcgg ctttgctgaa gtctaatgaa
    41401 ccatcagcat tattgaccgc aatatcctgt tcatcaggca cgcacttata gcaagtaata
    41461 atcttcattg catctccaga aatcatgaag gaaacgtcgt tcatatcggt ggtgttaatt
    41521 acagatatca cgccctttaa tttggaaata aaactttaat caccaatatt gaaaatgtca
    41581 cacgcagata aatccaactt tcaatattgt taagttcctc accaatattg aaaacatcgc
    41641 gtagcaaaaa gaaattttca atattgcttt atggatcacc aatattgaaa gtcagatgct
    41701 atttttttac tcacaacaca acataacaaa ctgattaata atcaaaatta acgagaaaag
    41761 tgccctctat tcacccgaaa caaaaatgtg ataacaatca cagaatgcag cttattgaat
    41821 acccattatg agttagcc
//
LOCUS       DAZGOB010000031        41550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGOB010000031 DAZGOB010000000
VERSION     DAZGOB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.031"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            247..1344
                     /gene="mltA"
                     /locus_tag="ACHLIF_001711"
     CDS             247..1344
                     /gene="mltA"
                     /locus_tag="ACHLIF_001711"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO9605744.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1421..2227
                     /gene="tcdA"
                     /locus_tag="ACHLIF_001712"
     CDS             1421..2227
                     /gene="tcdA"
                     /locus_tag="ACHLIF_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO9605745.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2278..2721)
                     /gene="csdE"
                     /locus_tag="ACHLIF_001713"
     CDS             complement(2278..2721)
                     /gene="csdE"
                     /locus_tag="ACHLIF_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO9605746.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAIEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2721..3926)
                     /gene="csdA"
                     /locus_tag="ACHLIF_001714"
     CDS             complement(2721..3926)
                     /gene="csdA"
                     /locus_tag="ACHLIF_001714"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO9605747.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4118..4345
                     /gene="ygdI"
                     /locus_tag="ACHLIF_001715"
     CDS             4118..4345
                     /gene="ygdI"
                     /locus_tag="ACHLIF_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO9605748.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4696..5613
                     /gene="gcvA"
                     /locus_tag="ACHLIF_001716"
     CDS             4696..5613
                     /gene="gcvA"
                     /locus_tag="ACHLIF_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO9605749.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5632..6027
                     /gene="ygdD"
                     /locus_tag="ACHLIF_001717"
     CDS             5632..6027
                     /gene="ygdD"
                     /locus_tag="ACHLIF_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO9605750.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6020..7120
                     /gene="rlmM"
                     /locus_tag="ACHLIF_001718"
     CDS             6020..7120
                     /gene="rlmM"
                     /locus_tag="ACHLIF_001718"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO9605751.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7164..7895)
                     /gene="fucR"
                     /locus_tag="ACHLIF_001719"
     CDS             complement(7164..7895)
                     /gene="fucR"
                     /locus_tag="ACHLIF_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO9605752.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7953..8375)
                     /gene="fucU"
                     /locus_tag="ACHLIF_001720"
     CDS             complement(7953..8375)
                     /gene="fucU"
                     /locus_tag="ACHLIF_001720"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO9605753.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8377..9795)
                     /gene="fucK"
                     /locus_tag="ACHLIF_001721"
     CDS             complement(8377..9795)
                     /gene="fucK"
                     /locus_tag="ACHLIF_001721"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO9605754.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9904..11679)
                     /gene="fucI"
                     /locus_tag="ACHLIF_001722"
     CDS             complement(9904..11679)
                     /gene="fucI"
                     /locus_tag="ACHLIF_001722"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO9605755.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQRRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11712..13028)
                     /gene="fucP"
                     /locus_tag="ACHLIF_001723"
     CDS             complement(11712..13028)
                     /gene="fucP"
                     /locus_tag="ACHLIF_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO9605756.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13575..14222
                     /gene="fucA"
                     /locus_tag="ACHLIF_001724"
     CDS             13575..14222
                     /gene="fucA"
                     /locus_tag="ACHLIF_001724"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO9605757.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14250..15398
                     /gene="fucO"
                     /locus_tag="ACHLIF_001725"
     CDS             14250..15398
                     /gene="fucO"
                     /locus_tag="ACHLIF_001725"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO9605758.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15453..16208)
                     /gene="xni"
                     /locus_tag="ACHLIF_001726"
     CDS             complement(15453..16208)
                     /gene="xni"
                     /locus_tag="ACHLIF_001726"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO9605759.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16320..17687)
                     /gene="sdaB"
                     /locus_tag="ACHLIF_001727"
     CDS             complement(16320..17687)
                     /gene="sdaB"
                     /locus_tag="ACHLIF_001727"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO9605760.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17745..19034)
                     /gene="sdaC"
                     /locus_tag="ACHLIF_001728"
     CDS             complement(17745..19034)
                     /gene="sdaC"
                     /locus_tag="ACHLIF_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO9605761.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19591..20955)
                     /gene="ppnN"
                     /locus_tag="ACHLIF_001729"
     CDS             complement(19591..20955)
                     /gene="ppnN"
                     /locus_tag="ACHLIF_001729"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO9605762.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21067..21915)
                     /gene="queF"
                     /locus_tag="ACHLIF_001730"
     CDS             complement(21067..21915)
                     /gene="queF"
                     /locus_tag="ACHLIF_001730"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO9605763.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            21983..22528
                     /gene="syd"
                     /locus_tag="ACHLIF_001731"
     CDS             21983..22528
                     /gene="syd"
                     /locus_tag="ACHLIF_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO9605764.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23150..23479
                     /gene="yqcC"
                     /locus_tag="ACHLIF_001732"
     CDS             23150..23479
                     /gene="yqcC"
                     /locus_tag="ACHLIF_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO9605765.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23479..24261
                     /gene="truC"
                     /locus_tag="ACHLIF_001733"
     CDS             23479..24261
                     /gene="truC"
                     /locus_tag="ACHLIF_001733"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO9605766.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24279..24728
                     /gene="yqcA"
                     /locus_tag="ACHLIF_001734"
     CDS             24279..24728
                     /gene="yqcA"
                     /locus_tag="ACHLIF_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO9605767.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            25163..26515
                     /gene="gudP"
                     /locus_tag="ACHLIF_001735"
     CDS             25163..26515
                     /gene="gudP"
                     /locus_tag="ACHLIF_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO9605768.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26517..27857
                     /gene="gudX"
                     /locus_tag="ACHLIF_001736"
     CDS             26517..27857
                     /gene="gudX"
                     /locus_tag="ACHLIF_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO9605769.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27878..29218
                     /gene="gudD"
                     /locus_tag="ACHLIF_001737"
     CDS             27878..29218
                     /gene="gudD"
                     /locus_tag="ACHLIF_001737"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO9605770.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGKYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29450..32206)
                     /gene="barA"
                     /locus_tag="ACHLIF_001738"
     CDS             complement(29450..32206)
                     /gene="barA"
                     /locus_tag="ACHLIF_001738"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO9605771.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32263..33564
                     /gene="rlmD"
                     /locus_tag="ACHLIF_001739"
     CDS             32263..33564
                     /gene="rlmD"
                     /locus_tag="ACHLIF_001739"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO9605772.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33612..35846
                     /gene="relA"
                     /locus_tag="ACHLIF_001740"
     CDS             33612..35846
                     /gene="relA"
                     /locus_tag="ACHLIF_001740"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO9605773.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            35924..36172
                     /gene="mazE"
                     /locus_tag="ACHLIF_001741"
     CDS             35924..36172
                     /gene="mazE"
                     /locus_tag="ACHLIF_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO9605774.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36172..36507
                     /gene="mazF"
                     /locus_tag="ACHLIF_001742"
     CDS             36172..36507
                     /gene="mazF"
                     /locus_tag="ACHLIF_001742"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO9605775.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36578..37369
                     /gene="mazG"
                     /locus_tag="ACHLIF_001743"
     CDS             36578..37369
                     /gene="mazG"
                     /locus_tag="ACHLIF_001743"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO9605776.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37597..39234
                     /gene="pyrG"
                     /locus_tag="ACHLIF_001744"
     CDS             37597..39234
                     /gene="pyrG"
                     /locus_tag="ACHLIF_001744"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO9605777.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39322..40620
                     /gene="eno"
                     /locus_tag="ACHLIF_001745"
     CDS             39322..40620
                     /gene="eno"
                     /locus_tag="ACHLIF_001745"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO9605778.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(40680..>41550)
                     /gene="ygcG"
                     /locus_tag="ACHLIF_001746"
     CDS             complement(40680..>41550)
                     /gene="ygcG"
                     /locus_tag="ACHLIF_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO9605779.1"
                     /translation="RYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELT
                     HKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSDR
                     TVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQYP
                     TNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDLF
                     LFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSGG
                     GGSSGGGGASGRW"
BASE COUNT        10317 a        10054 c        11030 g        10149 t
ORIGIN      
        1 tatgagcccg acgagctacc aggctgctcc accccgcgtc cgtggatgcg cactatactc
       61 cgctcgcgtt ttcatgcaac ctttttttta ccccgatcac ggatttagca tgattttgaa
      121 caaaaacaaa ccactaaagc ctattttttg tgcaaaattt gcgtcaggga gaggctgctc
      181 atttcattaa acgcggcggt ttgttatctt cgttgcgcct tattttttaa cctgaagaag
      241 agaacaatga aaggacgttg ggtaaagtac cttcttatgg gcacggttgt ggcaatgctt
      301 gccgcctgct cttccaaacc aaccgatcgc ggacagcaat ataaagacgg gaaatttacc
      361 cagcctttct ctctggtgaa ccagccagat gccgttggcg cgccgattaa cgccggtgat
      421 tttgccgagc aaattaacca tatccgtaat tcgtcaccgc gtctgtatgg caaccagagt
      481 aatgtttata acgcggtgca agagtggctg cgcgcaggcg gtgatacccg caatatgcgc
      541 cagttcggca ttgatgcctg gcagatggaa ggtgccgaca actatggtaa cgtgcagttt
      601 acaggctatt acacgccggt aattcaggcg cgccataccc gccagggcga gttccagtat
      661 cctatttacc gtatgccgcc aaaacgtggt cgtctgccgt ctcgtgcgga gatctacgca
      721 ggggcgctga gtgataaata tattctcgct tacagcaact ccctgatgga taacttcatt
      781 atggatgtgc agggtagcgg gtatatcgac tttggtgatg gcagtccgct taactttttc
      841 agctatgcag ggaaaaacgg tcatgcctat cgcagcattg gtaaggtgct gatcgaccgt
      901 ggcgaagtga aaaaagaaga tatgtcgatg caggcgattc gtcattgggg cgaaacacac
      961 agtgaagccg aggttcgcga gctgctggaa cagaacccgt ctttcgtttt ctttaaaccg
     1021 caatcttttg caccggtgaa aggggcaagt gcggtgccgc tggttggccg tgcttctgtt
     1081 gcctccgatc gttcaattat tccgccaggt actaccttgc tggcagaagt gccgctgctg
     1141 gataataacg gcaaatttaa cggtcagtac gaattgcgtc tgatggtggc gctggatgtc
     1201 ggtggcgcaa tcaaaggcca acacttcgat atctatcaag ggatcgggcc ggaagccgga
     1261 catcgcgcag gttggtacaa ccactatgga cgtgtctggg tgctgaaaac cgccccgggc
     1321 gcaggtaacg tctttagcgg ctgatgtgtt attctgtata ccgtttttac ggatacgaca
     1381 gggtgaggga taacctcacc ctttttaatt ctgaggtgct atgtctgtgg taattagtga
     1441 tgcatggcgt cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag cgttgcaact
     1501 gtttgctgac gcgcatattt gtgtggttgg tatcggtggt gtcggttcct gggcggcgga
     1561 agcgctggcg cgcacgggga ttggcgcaat cacgcttatc gatatggatg atgtgtgcgt
     1621 caccaatacc aatcggcaaa ttcacgctct gcgcgataac gtcgggctgg caaaagcgga
     1681 agtcatggcg gagcgtattc gccagattaa cccggagtgc cgtgtaacgg tggtggatga
     1741 tttcgtgacg ccggataacg tagcgcagta tatgagcgtg gggtattcgt acgtgattga
     1801 tgccattgat agtgtacggc ccaaagcggc gctgattgct tattgtcggc gcaataaaat
     1861 cccgctggtc acgaccggtg gcgcgggtgg gcagattgac ccgacgcaga ttcaggttac
     1921 cgatctggcg aaaacgattc aggatccgct ggcggcgaag ttgcgcgagc gcctgaaaag
     1981 cgattttggc gtagtgaaaa acagtaaagg taagctcggc gtcgattgcg tgttttctac
     2041 tgaagcgctg gtgtacccgc agtcagacgg tactgtgtgt gcgatgaaag ccacggcaga
     2101 agggccgaag cggatggatt gtgcatctgg atttggcgcg gcaacgatgg tgaccgccac
     2161 ctttgggttt gttgcggttt ctcatgcgct gaagaagatg atggcgaaag cggcgcgtca
     2221 gggttaaaca atcaggtcgg atgcgacgcg aacgtcttat ccgacctggt tctgtgctta
     2281 aacctgcttc gtcgcagcga taatcgcctc gcttaacgca ttcaacccct ggctgcgtga
     2341 ggcgctaagc tgcccacgca atcccagctc atcaaacaat gccagcggcg actgtgcctg
     2401 caattcggcg gcggttttcc cctcaatggc agtcaacagc accgccagca ggccgcgcac
     2461 tatacgtcct tcgctgtcgc caaagaagtg cattttaccg ttttcagcca cggtatatcc
     2521 cagccagacg cggttttcgc atccggcaat ctctttagcc tgcgctttta actcgtctgg
     2581 caaagccgga agctgtttcc ccagcatgat caactggcga tatttatctt cccattgcgt
     2641 caacggtgcg aaggtattgc gtaacgtttc tgcggttacg gttgtgccga acggatgtcc
     2701 ggcgaattgc gggttcgtca ttaatccacc aataattcca gcgcgcggtc aacggcattc
     2761 accagcgcat ccacatcact ctttgtatta tatggcgcaa aagaggcgcg cagtgtgccg
     2821 gttacgccta attctgccag tagcggctga gcgcaatgtt gcccggcccg caaggcaata
     2881 ccgtactccg ccagcagcgt caccatatcg ctgtgatgaa cgccagcaaa atcaaaggcc
     2941 agcaggctgg aatcctggca gcggaatgaa cggaaacctg gacgtttcgc cagcgcatct
     3001 tccgccagtg ttgctaagct acggctccag ttttcggcct ggttgatatc gtaatctgcc
     3061 agccattcca gcgccgcgct taatcctatg acaccagcca catttggcgt tcctgcttcc
     3121 agtttccacg gcgcagattg agtcgtgaag ccgtcaaaac tcacttcgtg aaccattttg
     3181 ccgccgccca gccagggcga catcgcttcc agcagttctg atttgccata cagcacgccg
     3241 atacctgtcg ggccatacag tttgtgacct gaaaaagcat agaaatcaat attcagttgc
     3301 tgaacatccg cggggaaatg caccgccccc tgagcaccat caaccatcac caccatcccg
     3361 gctgaatgag caaaggtaat cgctcgcgcc agatccgggc aaccgccagt aacgttcgac
     3421 atctgaccca acgccagaat ccgactacgg ggagtaatca gttctggcaa caaatcgaca
     3481 tccggcagtc gctgcgcatt aagcggcaat ttcaccactt tggctccagt ttgttgggcg
     3541 accatcagcc aggggacgag gttggcgtgg tgttctgcca cgctgacaat aatctcatcg
     3601 cccagttgca gacgcggacg cgcatagcat tgtgcaacca tgttgatgga ttcagtggtg
     3661 ccgcgcgtcc agacgatagt tttatcatcc ggtgcattca gtaattgcgc cactttctct
     3721 cgtgcagctt cataacgcgc ggtcaggcgt tgggcttcgg caaactggct gcgatggacg
     3781 tttccggcgc tcaggctgta aaactgtcgg gtggcttcaa ccacggcttc aggtttaagc
     3841 gcggtcgcgg cgctgtcgag atagacgccc gcatcctgta gagcgggaaa ctgggcgcga
     3901 aactgcgcgg gattaaaaac gttcatggta ctcctcggct tgatgcaccg atcgtgacgc
     3961 aaaattcact ttgatacaag gagagtgcta accatcagaa ttatctggat atcctggcga
     4021 agccgcgtaa gtaacatatg ctgaatagtg ttaggcgtta attttagcct gttaatcaat
     4081 actattaaga tttattaatc gctttaagga gaagatgatg aaaaagactg ccgcaattat
     4141 ttctgcctgt atgctgactt ttgccctgag cgcctgttcc ggttcgaact atgtgatgca
     4201 caccaatgac ggacgtacca tcgtctctga cggcaaacca cagactgata acgataccgg
     4261 tatgatttcg tataaagacg ctaatggcaa caaacagcag atcaaccgta ctgacgtgaa
     4321 agagatggtc gaactggatc agtaattcac gatcgcaagg taaaaaaaag caccgcaatt
     4381 aggcggtgct acattaatca ctatggacag acagggtaaa tgtacaggaa gtgaaaaaag
     4441 gtagctttgc taccatggtc tgaatcgcag accaattgca aacacaacaa cacaacatca
     4501 caaccgtaag ccaaaagttc accagaacac gcattccgat aaaacttttc gttccggctc
     4561 aggaagtgcc gccactatag gtatttgctg gtagaagctc aacggacaat ttataatggc
     4621 tcagattaaa aaaactaata ggttacacag tgtgatctaa ttgttaaatt catttaacat
     4681 caaagtttaa aagccatgtc taaacgatta ccaccgctaa atgccttacg agtttttgat
     4741 gccgcagcac gccatttaag tttcactcgc gcagcagaag agctttttgt gacccaggcc
     4801 gcagtaagtc atcaaatcaa gtctcttgag gattttttgg ggctaaaact gttccgccgc
     4861 cgtaatcgtt cactcctgct gaccgaggaa gggcaaagct atttcctcga tatcaaagag
     4921 atattttcgc aattaaccga agcgacgcgt aaactccagg cccgtagcgc caagggggcg
     4981 ttgacggtca gtttactccc cagtttcgcc attcattggt tggttccgcg actttccagc
     5041 tttaattcag cttatccggg aattgacgtt cgaatccagg cggttgatcg tcaggaagat
     5101 aagctggcgg atgatgttga tgtggcgata ttttatggtc ggggcaactg gccggggcta
     5161 cgggtggaaa aactgtacgc cgaatattta ttgccggtgt gttcgccgct actgctgaca
     5221 ggcgaaaaac ccttgaagac cccggaagat ctggctaaac atacgttatt acatgatgcg
     5281 tcacgccgtg actggcagac atatacccga cagttggggt taaatcatat caacgttcag
     5341 caagggccaa tttttagtca tagcgccatg gtgctgcaag cggctattca cgggcaggga
     5401 gtggcgctgg caaataacgt gatggcgcaa tctgaaattg aagccggacg tcttgtttgc
     5461 ccgtttaatg atgttctggt cagtaaaaac gctttttatc tggtttgtca tgacagccag
     5521 gcagaactgg gtaaaatagc cgcctttcgc caatggatcc tggcgaaagc cgctgctgaa
     5581 caagaaaaat tccgctttcg ttatgaacaa taatttacgt agggtacgac catgaccagc
     5641 cgttttatgc tgattttcgc cgccattagc ggcttcattt ttgtggctct gggcgctttt
     5701 ggcgcgcatg tgttaagtaa aaccatgggg gccgttgaga tgggctggat ccagaccggc
     5761 ctcgaatacc aggcgtttca tacgctggcg atcttaggtc tggcggtggc aatgcagcgt
     5821 cgcatcagta tctggtttta ctggagtagc gttttcctcg cgttaggcac ggtgctgttc
     5881 agcggcagcc tttattgcct ggcactgtcc catctgcgtt tgtgggcgtt tgtcactccg
     5941 gttggcggcg tgagcttcct tgcgggctgg gcgttaatgt tagttggtgc tatccgttta
     6001 aagcgcaagg gcgtaagtca tgaataaggt tgtattgctg tgccgtccgg gctttgaaaa
     6061 agagtgcgcc gcagaaatta ccgataaagc cggccagcgg gaaattttcg gttttgcccg
     6121 cgtgaaagag aatgcgggtt atgtcattta tgaatgttat caacctgatg atggcgataa
     6181 gttaatccgt gagctgccgt tcagttcatt aatttttgcc cgccagtggt ttgtggtggg
     6241 ggaactcctg cagcatttgc cgccagaaga tcgtattacc cccattgtcg gcatgttaca
     6301 gggcgtagta gagaagggcg gtgaactgcg tgttgaagtt gccgatacca acgaaagcaa
     6361 agagttgctg aaattctgcc gtaaattcac cgtgccgcta cgcgctgcct tgcgcgatgc
     6421 cggggtgctg gcgaactatg aaacgccgaa gcgtccggtt gtgcatgtat tcttcattgc
     6481 accaggttgc tgctataccg gttactcata cagcaacaat aattcgccgt tctatatggg
     6541 cattccgcgc ctgaaatttc cggcagatgc gccgagtcgt tccacactca aactggaaga
     6601 ggcatttcat gtgtttattc ctgcggatga gtgggatgaa cgcctggcga acgggatgtg
     6661 ggcggtggat ttgggcgctt gcccaggcgg ctggacctac caactggtga agcgcaacat
     6721 gtgggtttat tccgtcgaca acggcccgat ggcgcaaagt ctgatggata ccgggcaggt
     6781 gacgtggctg cgggaagacg gtttcaaatt ccgtccgaca cgcagcaata tctcctggat
     6841 ggtatgcgat atggttgaaa aaccggctaa agttgcggca ttgatggcgc agtggctggt
     6901 taatggctgg tgccgtgaaa ccattttcaa tctcaaactg ccgatgaaaa aacgctacga
     6961 agaagtatca cacaatctgg cgtatattca ggcacagctt gatgaacatg gcataaatgc
     7021 tcagattcag gcgcggcagt tgtatcacga tcgcgaagaa gtgacggtgc acgtccgccg
     7081 catctgggct gcggtgggtg gtcgtcgcga cgagcgataa caggcaaggc cggggcgtcg
     7141 tctccggcct gctacccttt ccgtcaggct gttaccaaag aagttgcaac ctggcgctca
     7201 tcagaaataa tgtgcgttac ctcatccaga tgcccgatgc gggcttcccc tgaacgatta
     7261 aatttacttt tatcaatcaa taacaacgat tgcgcggcac gttttaatag catcgatttg
     7321 taatcagcgt tgatggcatt ggagtcccac agtgcgccgc tgctatcgat cccttcacaa
     7381 gaaaaaataa acagatcgat ttccagcgat ttaagttggg aaatcagcga gggattgacg
     7441 taacagccat atttgcgctc aagtgtgccg ccggaactga tcagttgaat gcgttcgcgt
     7501 ttaccgagtt catggcaaat cggatggcta ttggtgaaga cctgaatgtt gatgtcaggc
     7561 aactggcgtg ccagatacca gcaagttgaa ctggcgtcta aggctatcac catcccttct
     7621 tcaatccacg cgagcgcctc gcgcgcgata tctgctttat gcgcataatg gcttttcagc
     7681 cggatgtgaa agggatcgcc actgtcttga ttttgacggt ggatatattt agcgcgtcca
     7741 tgattgcgca gaattttacc ctgcgtctgt aattcattga gatcgcgacg aatggtttct
     7801 ttactgacct ttagctgttc agagagagct tccgtggtca ggctggtatg gttcagcagc
     7861 aggtcgacta tcgcttgctg gcgtgccgct ttcatatttt tcaccccatc attcgataac
     7921 cgcccgcgtc atgcgggcgt accggtatga gattacggtg ttaccccttt ttttaaaaga
     7981 atattcccgt acttcgctcg ttcgcctgtg ataacgatcg caaaggcttt ttgcgcccgt
     8041 tcataaaacg caaaacgatt gatgcggatg atgtcaggac acggggcttg tagtgaaagc
     8101 gcattacggt aacgtcgttc tacttcagga tcgagagtgt caccttctac cgctgccatc
     8161 atcaccagcg gcggggcata actgtccagc tcaaataacg ggataatcgc ctggagcaag
     8221 tcgctcacca acaggccatc agcgcggatc acctgcggtc ccatcgaatg ggcgggaaag
     8281 tgagcatcgg aaaaaataat ttcatctcca tgtcccatct ctgccagcac tttcaatagt
     8341 tcgggagaaa ttaacggcga aattgttttc agcatttcac acttcctcta taaattcagg
     8401 ttcagtttgc gggtagaaat aacggtactg ataatgaatc tgtgcacggg cttcttccgg
     8461 gctgttgaat tcccctacgc cataccaacc gaacagcgca gctcctgcga cggtcgtttc
     8521 ggcatcgtcg agaactttta ccggaatatc aagcatattg gctttaatct gattccacaa
     8581 tgtgttgcga cttcctccac cgactaacaa taattcagag gccttaaagt gcccgatttt
     8641 ttccagcatc tgtagattgc gctgtaattg cgcagttaac ccttccagcg ccgcgcgata
     8701 gaaatgcccc cgcgtggtat taagcgtcac tccttgccag ccagcgttct gacacgacaa
     8761 taaatcacac tgcattttta cgccatccgc gccaggcgcg atcagacgag cttcttcaat
     8821 caacatttgc cagggtgttt cagccgtcca gaacagtttt ctcacccatt ccagcacgcc
     8881 ggatgccagc cattgcatac ctgggttata caaccctgcc tggctatcca gttcgcaggt
     8941 ggaaccggcg tactgactta acagcgaagt atcaacctgg gcgctacgga ccattaaaat
     9001 ttcccatgta ccggaagaga gcacgggttc attttggtcg gcaccagcgc caaaaagggc
     9061 gaactgggta tcgtgacctg cggaaatcac cggtatgcca acgggtaagc cgagcattgc
     9121 tgcggcgctg ttctgtagcg taccaatctg ttcacccgct tccaccagac gagggaagag
     9181 tcggcgtgga ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt gctggatatc
     9241 cagcatctgg ctggttccgg ccatcgtgat atcagtagtg aattcgccgg ttaaacggtg
     9301 gttaatcagc gacgaaataa agagccaggc gtgcgcgcgt tccagcagtt gtggatgatt
     9361 ttctttcaac cacaccaact tatataacgt attgaaacta aaggctccga cgccagaaat
     9421 agcctgcaac cgctgtgcgg agatcaactg ttcaatattg tccataaccg ctgctgttcg
     9481 cggacatttc cagctaatga tcggatagag cagattgcct tgcttatcta ccagagcgcc
     9541 atccacacca aaggtggtga cggcgatacc gcggatgtgg cattcagtca gttcactatt
     9601 gatttgccga cagcaatcag caaagcgttg caaaatggcg tctaaagacc actggtgcca
     9661 ggtgttgttt tccatcgcga tatcgctggc attaggcgtt gaggcgcggg caacaatttt
     9721 tccctgccga ttaaccgcga tggccctgac attggtcgcg ccacagtcga gtaccaggat
     9781 aacttcttgt ttcataatcg ctcctgcaat atagccggat aacattgctt atccggctaa
     9841 ccactcttgc ttgttcaggc ccggtagccg gagcgaccgg gcatcacatc agggagtaat
     9901 gtattaacgc ttgtacaacg ggccgtagtt ctggcaagcg cggtaatcct ggccttcaat
     9961 atccatgccg tgcgcagccc aggcagaagg acgatacact ttggtctctt caacgttgtg
    10021 catacatacc gggatacgca gcatggaggc gagagtgata aagtctgcgc caacgtggcc
    10081 gatggtcaga accccatggt tagcgcccca gttcgccatt accgagtaca catccgtaaa
    10141 cggcccttta ccggtgaggc gcggtgcaaa ccaggtggtt ggccaggttg agttggtgcg
    10201 tttgttgagg atgtcatgca catccttcgg caattccacg ctccagcctt ccgcgatttg
    10261 cagtaccggt cccaggcctt tgatgatgtt gacacgggtc atggtgaacg ggacgccgcc
    10321 ttcggtaagg aagcgggaag agtaaccgcc gccacggaag tattcgtgga tcgccgggca
    10381 ccattcggta gcggcgaggc aagcgtcagc ctcttgctga gagatttccc agtgcggctt
    10441 catcgtcggg ttaccttcgc tgtcgcgttg tttacaggaa ccgtccagcg cagcagaacc
    10501 ggagttgatc aaatggatga tgccgtgttc tgccagtcca tcgagtttat gccccgttac
    10561 acgctcaatt gcttctggtg accagtaggt acgcacatcg gcaaatacct gagcggtgcc
    10621 ggtgagctgg tgacccatta gcattgccac gccgttaaga ctgtcgtttt cggtcgccac
    10681 gacaaagggt tcgcgcacgc cattccagtc aaatgaactg ttaaggatcg cttcggcggt
    10741 gtcaccattg ggatattgat cggtccagtg acgttgcccc tggaagcccg cagcgatggc
    10801 gttgtagcca agtgattctt ccacgcgacc aatatcggcc agtttgctgt tgccttgcat
    10861 catgtcgcgg atacacatcg ccatcagtaa actttcgcgc agaactgcgc ggcgttgctc
    10921 ggcattgcgt tgatactgtt tgttattttc atcttcgcca tagcggaagt ttttatcagc
    10981 ccaggccagt gccatttcca gttcggcttc gtcgtaaatc ttctgatcga tacggcgacg
    11041 cagttcggtc atatccaccg cctggacttt cattcccagc caggattcaa agaagttgtg
    11101 atcaacaatg gaaccggcga tacccatcga aacgccgccc agcgacagat agcttttacc
    11161 tttcatgctg gcgacggcca aaccggcgcg ggcaaagcgc agcagttttt cttcaacatc
    11221 ggcaggaatc gatgtgtcat cggcatcctg aacgtcatga ccgtaaatgg agaatgccgg
    11281 gatgcctttc tggctgtgag ctgccagagc cgctgccagg taaacagcgc cgggacgttc
    11341 agtgccgtta aagccccaaa tggctttcgg gcgggttgga tccatgtcga tggtttcact
    11401 gccatagcac cagcaaggcg ttaccgtaat ggtgaggcct acattctgac tgctgaattt
    11461 ttcttcgcaa gcagcggctt cagccatacc cgcgatacag gtatcggaaa tgacacactc
    11521 gacggcagct ccgcaggcat ggcgcagttt ctcggtcagc agtgcggccg tagctttcgc
    11581 catattcatt gtttgttctt caagcgactc acgaacaccc atgcgacgac cgtcaataac
    11641 cgggcggata ccaattttcg gtaagctgat ttttttcatt acagattcct cactttattc
    11701 ggaaaatatg ttcagttagt tgccgtttga gagcggaaac gggcaaagat aaagatgacc
    11761 gcgaagcaga gtgcggggat cagttcagca gtggggatgt tgcccgccgc gtcactgaca
    11821 aaacccatga ccggagtgac aataccgccg ccaatgatgg tcataacgat gaaggacgaa
    11881 ccgtatttgg tgtcctggcc gagattctta atgcccagcg agaagattgt tgggtactga
    11941 atcgacataa aggcgctgca taaagtcagg gctattaagc ccacatgacc gccagcgaag
    12001 gctgagatca ggcacagtgc catagcgatt aatgcgtagg cggccaggac tttgtgtggt
    12061 gcgaagcgac tgatgagcca ggtaccggtg aaacgaccaa taaagaagca caccatggtt
    12121 ccggttaaat agttagcggc aaagcctgca gtcatacctg gaatttcttc tacagcgtag
    12181 cgaatcaaat agctccagca ggccgtttgt gcgccaacgt agcagaattg cgctaatact
    12241 gcccagcgcc agtggcgaat acgcgccagg cgagaaagcg atgcggagaa cgatccttgt
    12301 ttggcgtcac tgtgattatc actctgcaat gccgggaatt tcgtcagcat gatcagcagg
    12361 gcgaccagta acacgatagc cacgatgatc atataaggtg tctgtaccga taataccagg
    12421 ctgtgtttat acgcactcaa ttgctctgga gacattttat cgagaacgtc ttgcgattga
    12481 tgtggcacgt tagacaaaat aagactttgc ccaaagacaa ccgcgataat tgcgccaaac
    12541 gagttaaatg tttgcgcaag atttaagcgg aagtgaccac tgctttccgg ccctaatacc
    12601 gtaacaaaag ggtttgcggc agtttccaga caacctaatc cggctgcaat aataaatagg
    12661 ccaactaaaa ataaggtgta gttcattatt tctgcagcgg gccagaataa tgcagcgccc
    12721 aaggcatata aaaataatcc ggtaataatc cctgctttat aactgagttt tttcatcaat
    12781 atcccagcag ggattgggat aatgaaataa ccaaagtaaa aggccgattg gatcaggcca
    12841 gcctggaaat ttgtcagcgt aaaagcctgc tggaattgag gtaataaaat gtcgttaagg
    12901 ttattggcta ccgcccaaag aaaaaacagt gagcacagca gcgcgaatgg aataatgtaa
    12961 cttctgcttt gccctgcatc tttatctacc gcacggtaac tctgcgtttg tattgatgtg
    13021 tttcccatag catcctctta ggttcagaag cttaagcacg taatcggtta tgttttcaaa
    13081 agtgcaaaac cagcgagttc gggcttcata tcagtgaata tttgcctgtc tgaaatgaac
    13141 tcatggattc attttcaaat aaaaaacgct ctgtaatatg acggcggtca cacttattag
    13201 ctggaaggtg actttatgtg actaccatca ctttaataat cgaaatttca tgcccgtttc
    13261 agaaaataaa tcataaaaaa cacaaaccag atgaccgtat taattgttta tgccgaaaac
    13321 gggcattcct ggcttgagtg aatagaaaaa tgaccgtttt gataaatatt tttaagagcg
    13381 gtcattttta atggggttat aaaaagagaa cttgcagtta tttactgtga ttagttgaac
    13441 caggtcacaa aatataagct tgtgaatttc tttctggtta atcaggaaga agatggtggg
    13501 taattaaacg gctaattcaa tagtgtgaaa ggaacaacat tattgccctg ttttgaatca
    13561 gagagaggta gctaatggaa cgaaataaac ttgctcgtca gattattgac acttgcctgg
    13621 aaatgacccg cctgggactg aaccagggga cagcggggaa cgtcagtgta cgttatcagg
    13681 atgggatgct gattacgcct acaggcattc catatgaaaa actgacggag tcgcatattg
    13741 tctttattga tggcaacggt aaacatgagg aaggaaagct cccctcaagc gaatggcgtt
    13801 tccatatggc ggcctatcaa agcagaccgg atgccaacgc ggttgttcac aatcatgccg
    13861 ttcattgcac ggcagtttcc attcttaacc gatcgatccc cgctattcac tacatgattg
    13921 cggcggctgg cggtaattct attccttgcg cgccttatgc gacctttgga acacgtgaac
    13981 tttctgaaca tgttgcgcag gctctcaaaa atcgtaaggc aactttgtta caacatcatg
    14041 ggcttatcgc ttgtgaggtg aatctggaaa aagcgttatg gctggcgcat gaagttgaag
    14101 tgctggcgca actttacctg acgaccctgg cgattacgga cccggtgcca gtgctgagcg
    14161 atgaagagat tgccgtagtg ctggagaaat tcaaaaccta tgggttacga attgaagagt
    14221 aatttcgtaa agcaacaagg agaaggatga tggctaacag aatgattctg aacgaaacgg
    14281 catggtttgg tcggggtgct gttggggctt taaccgatga ggtgaaacgc cgtggttatc
    14341 agaaggcgct gatcgtcacc gataaaacgc tggtgcaatg cggcgtggtg gcgaaagtga
    14401 ccgataagat ggatgctgca gggctggcat gggcgattta cgacggcgta gtgcccaacc
    14461 caacaattac tgtcgtcaaa gaagggctcg gtgtattcca gaatagcggc gcggattacc
    14521 tgatcgctat tggtggtggt tctccacagg atacttgtaa agcgattggc attatcagca
    14581 acaacccgga gtttgccgat gtgcgtagcc tggaaggact ttccccgacc aataaaccca
    14641 gtgtaccgat tctggcaatc cccaccacag caggcactgc ggcagaagtg accattaact
    14701 acgtgatcac tgacgaagaa aaacggcgca agtttgtttg cgttgatccg catgatatcc
    14761 cgcaggtggc gtttattgac gctgacatga tggatggtat gcctccagcg ctgaaagctg
    14821 cgacgggtgt cgatgcgctc actcatgcta ttgaggggta tattacccgt ggcgcgtggg
    14881 cgctaaccga tgcactgcac attaaagcga ttgaaatcat tgctggggcg ctgcgaggat
    14941 cggttgctgg tgataaggat gccggagaag aaatggcgct cgggcagtat gttgcgggta
    15001 tgggcttctc gaatgttggg ttagggttgg tgcatggtat ggcgcatcca ctgggcgcgt
    15061 tttataacac tccacacggt gttgcaaacg ccatcctgct accgcatgtc atgcgctata
    15121 acgctgactt taccggtgag aagtaccgcg atatcgcgcg cgttatgggc gtgaaagtgg
    15181 aaggtatgag cctggaagag gcgcgtaatg ccgctgttga agcggtgttt gctctcaacc
    15241 gtgatgtcgg tattccgcca catttgcgtg atgttggggt acgcaaggaa gacattccgg
    15301 cactggcgca ggcggcactg gatgatgttt gtaccggtgg caacccgcgt gaagcaacgc
    15361 ttgaggatat tgtagagctt taccataccg cctggtaaat gcgctgatgt gataatgccg
    15421 gatacgacgt ttgcggcgta tccggctcgc cgttaccgta ccaaccgcaa ttgctgtaaa
    15481 ttgccgtcga tatgcaaatc ggtttgtaag cgggcaatat cgcggcacag aaacgccatc
    15541 tctttatggg tttctaattt tttgcgccac ttttcggcaa ccgcatccag attctcatat
    15601 atcccttcca gactctgaaa ctcgaccagc agctgcgtgg cgctttttgg tccgattccc
    15661 gcaacacccg gtacctttga actgctgatc cccgcaagtc cccagtaatc gggcaactgc
    15721 tgcggttgaa cgccaaattc tttatcgata aatggcgcat ccagccaacg tttctggaag
    15781 taatcacgaa tacgtaatgt cggtgaaagt aactgacagt agcctttatc tgtcgaaaca
    15841 atcgttgcct gatgcccggc ctgtgtcact ttgaccgcca gcgtggcggc taagtcatcg
    15901 gcttcgttgc cgctggttga ccagcacggg acgccgcgtt gctcaaaggc ggcgcgtaat
    15961 gcaggcatct cgtcgtgcaa ctcttccggc attggcggtc gacccgcttt gtaatctggt
    16021 aaacgctgat gacgccagcc gctactgcgg ttttcatcat caaaaacggc gaccgcgtgg
    16081 gttggctggc tgtgcataat gagctgatcg agcgcatgct ggcaggtttc gacacagggc
    16141 gatccctgaa cggcatgaat gcgacgaata agattcagtg catcgacaat aagcaaatga
    16201 acagccacga taaccccctt aaagatcagg cgataagggt aacattcgat gccggaacag
    16261 gctacggctg gatgagaaat cgggaagagg cctcgcaaaa cgaggcctct ggagagcgat
    16321 taatcgcagg caacgatctt cattgccagg ccgccgcgag aggtttcgcg gtacttggcg
    16381 ttcatatctt tacctgtttc gtacatggtt tcgataactt tatcgaggca gacgcgcggc
    16441 tcgctggtac ggcgtagcgc catacgtgcg gcgttcaccg cttttactgc cgcaatggcg
    16501 ttacgctcga tgcatggcac ctggacctgt ccggcgaccg ggtcacacgt cagaccgagg
    16561 ttgtgctcca tggcgatttc cgccgcgatg cacacctgcg ccgggcttgc acctaacagt
    16621 tctgccagac cagccgccgc cattgagcac gccacaccaa cttcaccctg gcagccgact
    16681 tcagcaccag aaattgacgc gttcatctta taaagagaac caatggcact ggctaccagc
    16741 aggtaacgag ccagtgagtt agcgttcact tcgcggataa acttgtcgta gtacgccagc
    16801 actgccggga taatcccgca cgcaccgtta gtcggcgcag tcaccacgcg tccgccagca
    16861 gcgttctctt cgttcactgc caatgcaaac atgttgatcc agtcaacaac cgccatcggg
    16921 tcagtggtgg ttttatcctg gctgaccagc atccggcgta gtgccgcagc acggcgtgga
    16981 acgcgcagtt tgccaggcaa cacgccttcg gtggaaatac cgcgctcaat accgccacgc
    17041 atgacttccc atacgttcgc caggtgctgt tccagctctt ctttgctgtg cagcgccagc
    17101 tcatttttca tcatcaggcc agagagtgac agcccggttt cctgacaatg tttttgcaga
    17161 tcggctgctg aactgtacgg ataaggaact tcaaccggtg cgctattctg ctggccaaaa
    17221 tgctcttcat caacgataaa gccaccgcca atagagtagt aagtctggct gtaaacgact
    17281 ttatcgcccg ccagcgcggt aatgcgcata ccgttttcat gcagagaaag gttgtctgca
    17341 tggaagttca tgcactgatc aaccgggaac tccacttcat gctgaccgtt tgccagcatc
    17401 aggcgaccat gagtattcac atcctgaata aaaccaggga tggaatcgat atccacggta
    17461 tccggcaggt tacccgccag gcccataata atggcgatat cagtgtggtg gcctttacca
    17521 gtcagagaga gcgagccgta cacgtcaacc accacgcggg tcacgtcttt aagcaggtta
    17581 cgggcaatca gatcgtcggt aaattgttta cccgctttca ttggtccaac ggtatgagaa
    17641 ctggaagggc caatgccgat tttgaaaata tcgaatacgc taatcatagg aaatacatcg
    17701 cgttaaaacg gaggaagcgc cgcccgaaag cggcgcgaaa ggacttagct gaacagagag
    17761 tagaagattg cggagattgc aatcagaccc atcacgacaa cgaatacgtt gctgatgtga
    17821 ccgctgtact tacgcattgc cggtactttc tgaattgcgt acatcggcat caggaacagg
    17881 atcatcgcga tgattgggcc gcccagggtt tcaatcatac ccaggatgct cgggttcagg
    17941 gtggcaacaa tccaggtcgt taccagcatg aacagcgcag tgatacggtt cagcttgttg
    18001 atttcgatag acttaccttt accacgcaga gatttaatca ccataccgtt gaagccttca
    18061 cgtgcgccca ggtagtggcc gaggaaggat ttggtgatag cgataatcgc gataatcgga
    18121 gccatccacg cgataaccgg tgcgttaaag tggttagcta ggtaagacag aatcgagatg
    18181 ttctgctctt tagccgcagc caggtctgcc ggagtcaggc tcagtacgca gctgaagacg
    18241 aagaacatta cggtcagcac catcatgatg tgtgcgaatg ccaggatctt cgagcatttc
    18301 tgttctgcca tatcgccgta ctcttcacgc ttcgcaacgg cgaaagaaga gatgatcgga
    18361 gagtggttga acgagaacac cattaccgga attgccagcc acagggtcat ccacagaccg
    18421 tttccggttg cagatgcagt gtccagagac agcgtttcca gtgctgcgcc gttccactgc
    18481 gggatcaggt acagagccag cagcatcagt acgccaacaa acgggaatac cagaatactc
    18541 atcgctttaa cgatcatctg ctcaccaaag cgaacgatgg tcatcatacc cacgatcagg
    18601 atcagcgaca gaatcgcacg cggcggtggc gtcataccca gctggtgaga catgaagctt
    18661 tcaactgtat tggtgattgc cacgctataa accagcagga tcgggtagat agcgaagaag
    18721 tagagcaggg taatcagttt acctgcgcca ataccaaagt gttcttctac aacctcggtg
    18781 atgtcttcgc ccgggttttt accagacagt acgaagcgag tcaggccgcg gtgagcaaaa
    18841 aacgtcatcg ggaacgcaag gatagccatg atgatcagcg ggatcatacc accaacaccg
    18901 gcgttgattg gcaggaacag cacgcccgcg ccgattgccg tgccgtaaag gcccagcatc
    18961 cacatggtgt ctgtcttgcg ccaggcacta cgagagtctt tcgacgcaat cgtgctggtt
    19021 tgagtcgttt ccatctattt ctcctggagg aatgcaaata attcagcttt tagccagttc
    19081 agcccgggaa ggtgcctgac cgtaaaaatg aaatctgtct atcggcgatg gcttaatcat
    19141 ttaccgccgt aataatttga gggcggaaag atacacacaa gttatgtatc tatcagtgat
    19201 cttgatccca aatacaataa tctacttgga agatagatta tcccaacgct ttactctgta
    19261 aatcaaaagt aaaagagaat ttaagggcaa tatttgccca ttaaacgaca tctgacttct
    19321 tccggggcgc gcaatatatg taggttgaac tgaaaaaatc aatactttta gttaatcatg
    19381 gcgataattt ctttttcata gcgataaaat acggtttatg aaaataggga aacgtttgcg
    19441 ttgcaatata aattgtatga catattaact gtgatgatat ttgtcgcgtg agggtggtaa
    19501 gaaacattca tataacaaat gaaatgagtt gatgagtaag tgaccaaaaa cgagaaaacc
    19561 cggcaacaaa cgtcaccggg ggaggggaga ttacgtgcag atttcgtagc aagggatgta
    19621 ggctgagcct ggcaacttca tacgatgctg ggcaacaaaa ccctgtagca ggtcgtccat
    19681 acgacgcata atctctttat cgccgttgat tttgtaagga ccaaactctt caatggcgcg
    19741 aataccgact tcttttacgt tacccgccac aataccggag aacgcacggc gcaggtcggc
    19801 agccagcact tcaacaggtt gatccgggta aagcttcaga ttagccatat tctcgtgaga
    19861 cggctcaaac ggcatttgca aatctggtgc aatgcgcatt gaccagttaa agctgtaagc
    19921 gtcaccagtg tcgcggcgat tttctttcac cagcggcatc gattttttca tctgacgagc
    19981 gacttcagcg gcgtcatcaa tgatgatgcg gtaatggcgg cgcgcgtttt cgcccagcgt
    20041 atgtacgaca aactcgtcca gtacgcggaa gtagtcggcg ctctctttcg ggccggtgag
    20101 gatcaatggt aaaacctgat ctttgttggc cgggttcatt aaaattccca gcaaatacag
    20161 caactcttct gccgtaccca caccgccagg gaagataatg ataccgtgag cgatacggac
    20221 aaacgcttcc agacgttttt cgatatctgg catgatgatc aattcgttga ccagcgggtt
    20281 aggcggttca gcggcgataa tcgacggctc ggtcatacca ataaaacgac tgtctttgta
    20341 acgctgctgc gcgtgtccga ccgcagcacc tttcatcggc gcttccatcg ctcccggacc
    20401 acagccggtg cagatattca gctcacgcag gcccagctgg tttccgacgc gacgggcata
    20461 caaatactcg ttttcgttaa ttgagtgacc gccccagcag accaccatat ttggcgcttc
    20521 acccacatgc agcgcgcgag cgttacgcaa gatggaaaag accaggttag tgatgtggac
    20581 ggagttgtcg agattcaggt tgggaaaacg aacggtatta tggatttgcc cgtaaacgaa
    20641 gagaatgtct cgcagaaccg cgaacaagtt ggcctgcaaa gcgcgaataa ttcgcccatc
    20701 gacaaaagcc tcttccgggg gattaatcag ttccagcttt acgccgcgtt cacggcgcag
    20761 gacgttaata tcgaaatttt caaaacgaga cagcaattct ttgctgttat cggtcaaact
    20821 accggagttc agtacggcaa gtgaacagtt gcgaaacagt tgatagaggt cgctgctggc
    20881 ggtgcgttta agcatatcca cttccagctg cgacaacata tccatggagc caagcgggct
    20941 aatatgtgta atcaagaaaa actccttatg ggacgaatat cgtcattccc tttgattaca
    21001 atagccctgc ctgatgacct ttcacaacct tctgcgtgaa tccagcacgc aaaattgaga
    21061 aaaaatttat tgccgaacca gtcttgtggt cgatgggaca aaatcgctat tactgcgcca
    21121 cgggttaatg tccagaccgc cacgacgggt ataacgtgcg taaacgctta atttctctgg
    21181 ctggcagaag cgtaacaggt cattaaagat gcgttccacg cactgttcgt ggaactcgtt
    21241 gtgatgacgg aatgagacca ggtaacgcag cagtttttct ctgtcaattt ggcgtccacg
    21301 atactgaatt tggatcgaac cccaatctgg ttgatgggtg atcaggcagt ttgatttcag
    21361 caggtggctg acaagcgtct cttccactac tttttcaccg ctggtggcat tctccagata
    21421 gtcagtagtg aattcatagt tatcgatagt gatatcctgg tcatcaatgc aggtgccatt
    21481 gaaatgacct atcggctggc cttccagttc atcaagacga tataacgcca cgctaacctt
    21541 accctgggcg caagtgctta agtcgcgctc cagcgtctgg cgcacctcat cccagttatt
    21601 aaaacgcgtc tggttaaaac tgttgagata gagcttaaaa ctcttcgact caatcagatt
    21661 tgcgctggtg taatcaagtt caacgtgacc gacggcaacc tgcggcaaac ctttcgcatt
    21721 cagccaggaa agttcataca gcgtccagat atccgtaccg tgaaaaggca ggttatccgc
    21781 tttcagaccc agcgggtcgc gattcaggct gcgtggaacg ccttgcagta ggttggcgtc
    21841 ataggtatcc cggtaatcgg ttgattttcc aagagtcagg cccgcaagtg cctgatggtt
    21901 tgcataagaa gacatgtttc accgtcataa accagataaa ctaaacgaca agtgtatctg
    21961 gtttaagtga agagagaaat tagtggacga tttgaccgca caagccctga aagattttac
    22021 tgcacgctac tgcgatgcgt ggcatgaaga gcataaaagc tggccgttaa gtgaggaact
    22081 gtacggtgtt ccttcaccgt gcattatttc aactaccgaa gatgccgtat actggcaacc
    22141 ccagccgttt accggggaac aaaatgtaaa cgcggttgaa cgcgcttttg atattgtgat
    22201 acaacccaca attcatacct tttatacgac tcagtttgcc ggggatatgc acgcgcagtt
    22261 tggcgatatc aaactgacat tgctgcaaac ctggagtgaa gacgacttcc gtcgggtgca
    22321 ggaaaatctg attggtcatc tggtgactca gaaaaggcta aaactgccgc ccacgctatt
    22381 tatcgcgacg ctggaagaag agttagaggt catctcggtg tgtaatctct ccggtgaagt
    22441 gtgcaaagag acgctgggaa cacgtaaacg gacgcattta gcctcaaatc ttgcggaatt
    22501 ccttaatcaa cttaagcctc ttctgtaatc caaatacccc atctggttgt gagagatctc
    22561 ttacagactc tgtaggagat cgccaggaaa taagcgaata cttaaaaaga taagaatcgt
    22621 tattttcatt taaaatcaaa atgttgattg ttaattctta actttcatat gaaattttcc
    22681 ttaaggcatt gtctgtaagc gccttgtaag acttcgcgaa aaagacgatt ctatcttcgt
    22741 cgacagggag tcagacaacg aagtgaacat caggatgatg acacttctgc aggacacacc
    22801 aggatggtgt ttcagggaaa ggcttctgga tgaagcgaag aggatgacgc aggacgcgtt
    22861 aaaggacacc tccaggatgg agaatgagaa ccggtcagga tgattcggtg ggtcaggaag
    22921 gccagggaca cttcaggatg aagtatcaca tcggggtggt gtgagcagga agcaatagtt
    22981 caggatgaac gattggccgc aaggccagag gaaaagttgt caaggatgag cagggagcaa
    23041 caaaagtagc tggaatgctg cgaaacgaac cgggagcgct gtgaatacag tgctcccttt
    23101 ttttattcct gctatccttc gcggcagttt ttcttattga ggttgcttta tgaccactca
    23161 tgaccgcgtt cgtctccagt tgcaggcgct tgaagcgtta ctgcgtgaac atcagcactg
    23221 gcgaaacgat gaaccccagc cgcatcaatt taatagtacc caaccgttct ttatggacac
    23281 catggaacca ctggagtggt tgcagtgggt gctgatcccg cgaatgcacg atttgttaga
    23341 taacaaacag ccgttaccgg gcgcttttgc cgttgcaccc tattatgaaa tggcgctggc
    23401 gacggaccat ccgcaacgcg cgcttattct ggctgagctg gaaaaactgg atgctctttt
    23461 tgcggatgat gcgagctaat gctggaaata ctctatcagg atgaatggct ggttgcggta
    23521 aataaaccct ccggctggct ggttcaccgc agctggctgg atcgcgacga gaaagtagtg
    23581 gtcatgcaaa ccgtgcgtga tcagattggc cagcacgttt ttaccgctca tcgtctggac
    23641 cgacccactt ctggtgtgtt gttgatggga ttatccagcg aggccggacg gctgctggca
    23701 caacagtttg aacagcacca aatccagaaa cgttaccatg cgattgtgcg cggctggttg
    23761 atggaagaag cagttctgga ttatccactg gtggaagaac tggacaaaat cgctgataaa
    23821 tttgcccgcg aagataaagg ctcgcagcca gcagtgacgc attatcgcgg tctggcgacc
    23881 gtagaaatgc cggtagcgac cggacgttac ccgaccacgc gctacggcct ggtggaactg
    23941 gagccgaaaa ccggacgcaa acatcagctt cgccgacatc ttgcccattt gcgtcatccg
    24001 attattggcg atagcaaaca tggcgattta cgccagaatc gcagcggtgc tgagcatttt
    24061 ggcctccagc ggttaatgct acatgccagt cagttgtcat tgacgcatcc ttttactggc
    24121 gagccgctga ctattcacgc gggcctggac gacacctgga tgcaggcgtt atcacaattt
    24181 ggctggcgcg gtctgcttcc tgaaaatgaa agggttgagt tcagtgcgcc atcgggtcag
    24241 gatggagaga taagttccta atccagggag aaaacggtat ggcggaaatt ggtatttttg
    24301 tcggcaccat gtacggaaat tcgctgttag tggcggaaga agcagaagca attctgaccg
    24361 cacagggcca caaagcaacg gtatttgaag atcctgaatt aagcgactgg ctgccctatc
    24421 aggataagta tgttctggtg gttacgtcca cgaccgggca gggcgacctt cctgatagca
    24481 ttgtgccgct ctttcaggga atcaaagata gtctgggctt ccagccgaat ctgcgttatg
    24541 gcgtgattgc gctcggcgac agcagttatg taaatttctg caatggcggc aaacagttcg
    24601 atgccttgtt gcaggaacag agcgctcagc gggttggcga aatactgttg attgacgcca
    24661 gtgagaaccc ggaaccggaa acggaatcaa atccgtgggt cgaacagtgg ggcacgctgt
    24721 tgtcctgata tgttcagcga gcggtaaatg tcgttttagc ggtgctgaat cgaatctttt
    24781 caggcaaatg ccagtaaaaa ctgcttcata gcgcggattt ttactggcgt ttgcctggag
    24841 tcaagcgatc catttcatac tcttctttat ttcttcgttt ttacccttcc tttcttgttc
    24901 ttgttttcat ttccgtgaag tggattccac cgtccagggc taatgccaaa atcgggcctc
    24961 attgaacgca ttaatgttgt gttgttgcac ggtgagccgc tatggcgcgc tttttatact
    25021 gctattgcca gatataaaca cgcgccgtat tcggcgaacg acctataaaa acggcaaaaa
    25081 acaccctacg tcacctctga tttcctggcg atgtcgcagt ccagagtgag cgtggctaac
    25141 gcgaattttc aggagtgcaa caatgagttc tttaagtcag gctgcgagca gtgtggaaaa
    25201 acgcacgaat gctcgttact ggatagtggt gatgttgttt atcgtcacat ccttcaacta
    25261 cggcgaccgc gctacgcttt ctattgctgg ttcggaaatg gccaaagata tcggccttga
    25321 tcccgtggga atgggctatg tgttctctgc cttctcatgg gcttatgtta tcgggcagat
    25381 ccctggtggt tggttgctgg atcgttttgg ttcaaaacgc gtctacttct ggtcgatctt
    25441 tatctggtcg atgtttacct tgctgcaagg cttcgtcgat atctttagtg gattcggcat
    25501 tatcgttgcc ctgtttacgc tgcgcttcct ggtcgggctt gctgaagcgc catctttccc
    25561 cggcaacagt cgcattgttg cggcctggtt tccggcgcag gaaaggggaa cggcggtgtc
    25621 gatttttaac tccgctcaat acttcgcaac ggtgatcttc gcgccgatta tgggctggct
    25681 gacgcatgaa gtgggctggt cacacgtctt cttctttatg ggcggtctgg ggattgtcat
    25741 cagctttatc tggttgaaag tcatccacga gccaaatcaa catccggggg taaatcagaa
    25801 agagctggag tacatcgccg cgggtggcgc gctgatcaat atggatcagc aaaacaccaa
    25861 agttaaagtg ccgttcagcg tgaagtgggg gcagatcaaa caactgctcg ggtcacggat
    25921 gatgatcggc gtttatatcg gtcagtactg tatcaacgcc ctgacttact ttttcattac
    25981 ctggtttccg gtttatctgg tgcaggcgcg tgggatgtcg attctgaaag cgggctttgt
    26041 ggcttccgtt ccggcggttt gcggttttat cggcggtgtg ctgggtggga ttatttccga
    26101 ctggctgatg cgccgcacgg gatcgctgaa cattgcgcgt aaaacaccga tcgtaatggg
    26161 catgttgctg tcgatggtga tggtgttctg caactacgtc aacgttgagt ggatgatcat
    26221 cggctttatg gcgctggcct tcttcggtaa gggcatcggg gcgctgggtt gggcagtaat
    26281 ggcagatacc gcgccaaaag agatcagcgg tctttccggt ggcctgttca acatgttcgg
    26341 taacatttct ggcatcgtca cgccaatcgc aattggttat atcgttggca cgactggctc
    26401 ctttaatggg gcgctgattt atgttggtgt tcatgcctta atcgcggtac tgagctacct
    26461 ggtgctggtg ggcgatatca agcgtatcga gctgaaacct gtcgcggggc aataagatga
    26521 cgacacaatc cagtcctgtt attactgata tgaaagtcat tccggtcgcc gggcacgata
    26581 gcatgttgct taatattggt ggggcacata acgcatattt cacccgcaat attgtggtac
    26641 tcaccgataa cgccgggcat accggcattg gtgaagcgcc gggcggagag gtgatttatc
    26701 aaacactggt cgatgctatt ccgatggttc tgggccagga agttgcgcgc ctgaataaag
    26761 tggttcagca ggtgcataaa ggtaatcagg cagccgattt tgataccttc ggcaaaggtg
    26821 cctggacttt tgaattgcgc gttaatgccg tggcggcgct ggaagccgcc ttgcttgacc
    26881 tgctaggtaa ggcgctgaat gttccggtct gcgaactgtt ggggccaggc aagcaacgcg
    26941 agactattac cgtcctcggt tatctgtttt atatcggtga tcggaccaaa accgatcttc
    27001 cttatctgga aaatacgccg ggcaaccatg agtggtatca gttgcgccat cagaaagcga
    27061 tgaacagcga agccgttgtg cgtctggcgg aagcctcaca ggatcgctat ggctttaaag
    27121 atttcaaact taagggcggg gtgttacctg gcgagcaaga aatcgacact gttcgggcat
    27181 tgaagaaacg cttccctgat gcgcggatta ccgttgatcc caacggtgcc tggctacttg
    27241 atgaagccat ttctctatgc aaagggctga atgatgttct tacctatgcc gaagatccat
    27301 gcggcgcaga acagggtttc tccggtcgtg aagtgatggc ggagtttcgg cgggcgaccg
    27361 gcttgcccgt cgcgactaac atgatcgcca ccaactggcg cgaaatgggt catgcggtga
    27421 tgctcaatgc ggtagatatt ccacttgccg atccgcactt ctggacgctt tccggtgcag
    27481 tccgtgtggc gcagctttgc gacgactggg ggctgacctg gggctgccat tctaataacc
    27541 atttcgatat ctctctggcg atgtttaccc atgtgggcgc ggcggcaccg ggtaatccta
    27601 ccgctatcga tacccactgg atttggcagg agggcgattg tcgcctgacc aaaaatccgc
    27661 tggagattaa aaacggaaaa attgccgttc ctgatgcgcc cggtctgggc gtggaactgg
    27721 actgggaaca ggtacaaaag gcacatgagg cctataaacg tctgcctggc ggtgcgcgta
    27781 acgacgcagg tccgatgcag tacctgatcc ccggctggac ctttgaccgt aaacgtcccg
    27841 ttttcggccg tcattgattc tgaaaaagga cataaatatg agttctcaat ttacgacgcc
    27901 tgttgttact gaaatgcagg tcatcccggt ggcgggtcat gacagtatgc tgatgaatct
    27961 gagtggtgca cacgcaccgt tctttacgcg taatattgtg attatcaaag ataattctgg
    28021 tcacactggc gtaggggaaa ttcccggcgg cgagaaaatc cgtaaaacgc tggaagatgc
    28081 gattccgctg gtggtaggta aaacgctggg taaatacaaa aacgttctga cgctggtgcg
    28141 taatactttt gccgatcgtg atgctggtgg gcgcggtttg cagacatttg atttgcgtac
    28201 cactattcat gtagttaccg ggatagaagc ggcaatgctg gatctgctgg ggcagcatct
    28261 gggggtaaac gtggcatcgc tgctgggcga tggtcaacag cgtagcgaag tcgaaatgct
    28321 cggttatctg ttcttcgtcg gtgatcgcaa agccacaccg ctgccgtatc aaagccagcc
    28381 ggatgactca tgcgactggt atcgcctgcg tcatgaagaa gcgatgacgc cggatgcggt
    28441 ggtgcgcctg gcggaagcgg cgtatgaaaa atatggcttc aacgatttca aactgaaagg
    28501 cggtgtactg gccggggaag aagaggccga gtctattgtg gcactggcgc aacgcttccc
    28561 gcaggcgcgt attacgctcg atcctaacgg tgcctggtcg ctgaacgaag cgattaaaat
    28621 tggtaaatac ctgaaagggt cgctggctta tgcagaagat ccgtgtggtg cggagcaagg
    28681 tttctccggg cgtgaagtga tggctgagtt ccgtcgcgca actggcctgc cgaccgcaac
    28741 caatatgatc gccaccgact ggcggcagat gggccatacg ctctccctgc aatccgttga
    28801 tatcccgctg gcggatccgc atttctggac gatgcaaggt tcggtacgtg tggcgcaaat
    28861 gtgccatgaa tttggcctga cctggggttc acactcaaac aaccacttcg atatttccct
    28921 ggcgatgttt acccatgttg ccgccgctgc accgggtaaa attactgcta ttgatacgca
    28981 ctggatctgg caggaaggta atcagcgcct gaccaaagaa ccgtttgaga tcaaaggcgg
    29041 tctggttcag gtgccggaaa aaccggggct gggtgtagaa atcgatatgg atcaagtgat
    29101 gaaagcccat gagctgtatc agaaacacgg gcttggcgcg cgtgacgatg cgatgggaat
    29161 gcagtatctg attcctggct ggacgttcga taacaagcgc ccgtgcatgg tgcgttaaga
    29221 cagaaaacca aaaccggctc tatctggagc cggcttgaga ctgatgctaa acgcaaaata
    29281 gcctgttgtg ctatgtatca ggtctacatc attttttaat tcattgaatt taattggttt
    29341 tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa cacaggcact ttgtcaccaa
    29401 tctgaaacca gcgtcataaa aagccgattg ctactcgaca agacgtccgt tacccgagaa
    29461 ttttgctggc ttcgcgcgct acattatcca tctcgtccaa cagttccagc agctccggct
    29521 ccaaatcttc ttctttagta ccgctacgta gctgttgttc gatcagttgg cagagattct
    29581 tcatacgcgg cacaccgcta tagccgcaac tgccatgcag tttatgaatc aaatcaacca
    29641 ggccttccgg gttttctcca accagctgtt cctcaacttt gttgcgaact tcaggcagga
    29701 aatcgagtaa catttgcagc atatcgcgcg ctaaatcggt ttttcctgct gcctggcgta
    29761 gtgccagttg ccagtcgagg gtcgcattcg ggttcaccac aatttcgttg acttcgggcg
    29821 tcacgacgcg agaggaaata ccgctgccag gcttgtagcg caacaacaaa ttatgcaatc
    29881 gctcttcttc aatcggtttc gccagataat cgctcatccc tgcgccaagc agcttctctt
    29941 tttgcccggc cattgcatgc gccgttaccg cgataaccgg cgtttgttgc tgatggggga
    30001 gctggtggat gagctcgcag gcccgaatgc catccatgtc aggcatttga atatccatta
    30061 agatcaaatc gaacggcatc tgtttcgccc gttcaaccgc ctgatgcccg ctatcgcaaa
    30121 gttccacatg ttgcaccata tcttccagca atgcgccgat aagtttcagg ttagcggggt
    30181 tgtcatcaac tgccatgact gtcattgcca gcttgctttc atcggttaca ggcaaaagcg
    30241 tgttttgttt gtgatgacaa aattccgtca gggcaggcaa caggcgcgta ggtgttaatg
    30301 gtttcagcag acacgcgccg ataccatctt gcttgagttt ttctgcattg acttgtgcat
    30361 ggcaaggaag tgccagcatc aggaaatcgg tcatcgatac cgctttcgct aatcgctcat
    30421 gttgcattgt tagcggctcg cggaaggtca ccgcgatgcc taacaacatc atgtcgtaat
    30481 gcgcgggagg cagcgcggag aacgttgggc tataaaccac ttccagcggc gtttcactta
    30541 aaatatccag cgtgcattgc gctgctgcgg agtttggttc gacataggcc aggcgcttac
    30601 cggcgaggca ctgggtggat ggcccttcga taataatgtt cgggttcaga tcgagattaa
    30661 tgtggaacca gaaagttgaa ccgcgattcg gctggctatg gaacgaaata tcgccgccca
    30721 tttcattaac cagtttttgt gtaatcacca gccccagacc ggtgccacca tgacggcggg
    30781 aaatactggc atcagcctgt cggaaggcct ggaataagcg cgattgatcg cgttcaggaa
    30841 taccaatgcc ggtatcccga atctgcactt caatctgcac tttggtatta ctcagcgcac
    30901 gtttttctac cagaatatca atgttgccat tctcggtgaa tttaattgca ttccccacca
    30961 ggttagtgat gatttgctgt aatcgcagtg ggtcgccgat cacgttatca ggcacgtcgc
    31021 ttttaatatt gagcgtcagt tccaaccctt tatcgtgaga agaatgtgcc agcagagtaa
    31081 cgacttcatc cagcgtgctg cgtagtggga atggaatact ttccagaatc agcttacctg
    31141 cttccagttt cgagaagtcg agaacatcat taataattgc cagcaaatta tttgccgaac
    31201 gttcaatcgt attcaggtga tcgcgctgcg ttggtgttaa ttctgttttc agcgtcaggc
    31261 gggtaaagcc aataacacca ttcagtggtg tacgcagctc gtgtgacata tttgccagaa
    31321 actcggattt aatacgcgcc gcttcctggg cgcgcttttt cgccagatct aactcaacgt
    31381 tctgaatttc catctgctcc agcgtttcac gcagatcgga cgtcgcctgg tcgatattgt
    31441 gctgcatctc ttcgtgataa gcagccagcg acattgccat cgagttgata ccgtttttca
    31501 gcatatccag ctcgccgagc ataaatcctt ccacccggct gtcgagttgc ccgcgacgga
    31561 tgcggtcgac ggtattcacc atgttgcgaa tcggaccggt tacatcgcgc attaagcgcc
    31621 agccaaaaat aagcgcaata ccgatacaaa acagcatcat cacgctggaa ataaagatct
    31681 ctttatattg ctgcaagcga accgatttaa gatccagctc cagcgcaata tatcccaaca
    31741 tattttgact atttttggca tcgctactgg gcgattcgtc gggggagtaa ctctcagaaa
    31801 taatcggcgt gcggaggatc ataatatcgc cgtcacgagt gacagtgagc tggcgaggaa
    31861 acggcacgtt gctgccgagc tgcattgatg agggatcaag atgaaaattg gaggtgacaa
    31921 agagtcggtt attttcatca taaaccgaaa tcgcgcgaac aatatcggaa tggcgacgat
    31981 gcagtacgct tattaactga ccgatagatt cgcgattttg caggctcatg ccatattcag
    32041 tagaaactgc aagcggctca ataatgctgg caccggcatc ttccagttga cgctgcaagt
    32101 cgttatagcg atgcacgacg aaaaagatac tcagcaataa accaataagg acggtcggtg
    32161 ccaggatcag aatcatcatg cgtgcgcgca ggctgtagtt ggtcatggag ttccgttatg
    32221 ggacaattaa ggtcacactg ttaaattgag aaagtctcgc caatggcgca attctactct
    32281 gcaaaacgac gcacgacgac gcgtcagatc ataaccgttt cagtcaacga cctcgactct
    32341 tttggtcagg gcgtggcgcg acataacggc aaaacgttat ttatccccgg attattgccg
    32401 caggaaaacg cggaagttgc tgttactgaa gataaaaaac agtatgcccg cgctaaagtc
    32461 gtacgccggt taagcgatag cccggaacgc gaaacaccac gctgtcctca ttttggcgta
    32521 tgcggcggct gtcagcaaca acacgccagc gtggatttac agcagcgaag caaaagtgcg
    32581 gcactcgccc gattaatgaa acacgatgtc tctgaagtga tcgccgatgt tccctggggc
    32641 tatcgccgcc gcgcgcgttt aagtctgaac tacttaccga aaacacagca acttcagatg
    32701 gggtttcgca aagcgggctc cagtgacatt gtcgacgtta aacaatgccc cattttagtg
    32761 ccccaacttg aagcattgct gcccaaagtc agggcatgcc tgggcagctt acaagttatg
    32821 cgccatcttg gtcatgttga actggtacag gcaaccagcg gcacgctgat gattttgcgc
    32881 cataccgcac cgctaagttc ggcagatcgc gaaaaactgg aacgcttttc gcattctgaa
    32941 ggcctggatc tgtatctcgc ccccgatagt gagatactcg aaaccgtctc tggtgagatg
    33001 ccctggtatg actcaaacgg gttgcgctta acttttagcc cgcgcgattt tattcaggtc
    33061 aatgcgggtg tgaaccaaaa aatggtagcg cgtgcgttgg aatggctgga tgtgcaacct
    33121 gaagatcgcg tactggatct gttctgcggt atgggcaact ttacactgcc attggcgaaa
    33181 caagcagcca gtgtggtcgg tgtagaaggt gttccggcgc tggtggaaaa aggccagcag
    33241 aatgcgcgtc ttaatggttt acagaatgtg acgttttatc acgaaaatct tgaagaagat
    33301 gtcacaaagc agccgtgggc gaaaaacggc ttcgataaag tgttgctgga cccggcgcga
    33361 gcaggtgccg caggtgttat gcagcaaatt ataaaactgg aacctattcg tatagtttat
    33421 gtatcctgta accctgcaac gctggctcgg gatagcgaag cgttattaaa agcaggatat
    33481 accattgcgc gactggcgat gctggatatg ttcccacaca cgggacatct ggaatcgatg
    33541 gtacttttct cgcgcgttaa atagttacga tttgccgatt tcggcaggtc tggtccctaa
    33601 aggagaggac aatggttgcg gtaagaagtg cacatatcaa taaggctggt gaatttgatc
    33661 cggaaaaatg gatcgcaagt ctgggtatta ccagccagaa gtcgtgtgag tgcttagccg
    33721 aaacctgggc gtattgtctg caacagacgc aggggcatcc ggatgccagt ctgttattgt
    33781 ggcgtggtgt tgagatggtg gagatcctct cgacattaag tatggacatt gacacgctgc
    33841 gggcggcgct gctgttccct ctggctgatg ccaacgtagt cagcgaagat gtgctgcgtg
    33901 agagcgtcgg taagtcggtc gttaacctta ttcacggcgt gcgtgatatg gcggcgatcc
    33961 gccagctgaa agcgacgcac actgattctg tttcctccga acaggtcgat aacgttcgcc
    34021 ggatgttatt ggcgatggtc gatgattttc gctgcgtggt catcaaactg gcggagcgta
    34081 ttgctcatct gcgcgaagta aaagatgcgc cggaagatga acgcgtactg gcggcaaaag
    34141 agtgcaccaa tatctacgcg ccgttggcga accgtcttgg gattgggcaa ctgaaatggg
    34201 agctggaaga ttactgcttc cgttatctcc acccgaccga atacaaacgc atcgcaaaac
    34261 tgctgcatga acgccgtctc gaccgcgaac actatatcga agagtttgtc ggccatctgc
    34321 gcgctgagat gaaagctgaa ggcgttaaag ctgaagtgta tggtcgtccg aaacacatct
    34381 acagtatctg gcgcaaaatg cagaaaaaga acctcgcctt cgatgagctg tttgatgtgc
    34441 gtgcggtacg tattgtcgcc gagcgtttac aggattgtta tgccgcactg gggatagtgc
    34501 acactcacta tcgccacctg ccggatgagt ttgacgatta tgtcgctaac ccgaaaccaa
    34561 acggttatca gtctattcat accgtggttc tggggccagg tggcaaaacc gttgagatcc
    34621 aaatccgcac caaacagatg catgaagatg cagagttggg tgttgctgcg cactggaaat
    34681 ataaagaggg cgcggcagct ggcggcgcac gttcgggaca tgaagaccgg attgcctggc
    34741 tgcgtaaact gattgcgtgg caggaagaga tggctgattc cggcgaaatg ctcgacgaag
    34801 tacgcagcca ggtctttgac gaccgggtgt atgtctttac gccgaaaggt gatgtcgttg
    34861 atttgcctgc gggatcaacg ccgctggact tcgcttacca catccacagt gatgtcggac
    34921 accgctgcat cggggcaaaa attggcgggc gcattgtgcc gttcacctac cagctgcaga
    34981 tgggcgacca gattgaaatt atcacccaga aacagccgaa ccccagccgt gactggttaa
    35041 acccaaacct cggttacgtc acaaccagcc gtgggcgttc gaaaattcac gcctggttcc
    35101 gtaaacagga ccgtgacaaa aacattctgg ctgggcggca aatccttgac gacgagctgg
    35161 aacatctggg gatcagcctg aaagaagcag aaaaacatct gctgccgcgt tacaacttta
    35221 atgatgtcga cgagttgctg gcggcgattg gtggcgggga tatccgtctc aatcagatgg
    35281 tgaacttcct gcaatcgcaa tttaataagc cgagtgccga agagcaggac gccgccgcgc
    35341 tgaagcaact tcagcaaaaa agctacacgc cgcaaaaccg cagtaaagat aacggtcgcg
    35401 tggtggtcga aggtgttggc aacctgatgc accacatcgc gcgctgctgc cagccgattc
    35461 ctggagatga gattgtcggc ttcattaccc aggggcgcgg tatttcagta caccgcgccg
    35521 attgcgaaca actggcggaa ctgcgctccc atgcgccaga acgcattgtt gacgcggtat
    35581 ggggtgagag ctactccgcc ggatattcgc tggtggtccg cgtggtggct aatgatcgta
    35641 gtgggttgtt acgtgatatc acgaccattc tcgccaacga gaaggtgaac gtgcttggcg
    35701 ttgccagccg tagcgacacc aaacagcaac tggcgaccat cgacatgacc attgagattt
    35761 acaacctgca agtgctgggg cgcgtgctgg gtaaactcaa ccaggtgccg gatgttatcg
    35821 acgcgcgtcg gttgcacggg agttaggccg aaatttgctc gtatctacaa tgtagattga
    35881 tatatactgt atctacatat gatagcggtt tgaggaaagg gttatgatcc acagtagcgt
    35941 aaagcgttgg ggaaattcac cggcggtgcg gatcccggct acgttaatgc aggcgctcaa
    36001 tctgaatatt gatgatgaag tgaagattga cctggtggat ggcaaattaa ttattgagcc
    36061 agtgcgtaaa gagcccgtat ttacgcttgc tgaactggtc aacgacatca cgccggaaaa
    36121 cctccacgag aatatcgact ggggagagcc gaaagataag gaagtctggt aatggtaagc
    36181 cgatacgtac ccgatatggg cgatctgatt tgggttgatt ttgacccgac aaaaggtagc
    36241 gagcaagctg gacatcgtcc agctgttgtc ctgagtcctt tcatgtacaa caacaaaaca
    36301 ggtatgtgtc tgtgtgttcc ttatacaacg caatcaaaag gatatccgtt cgaagttgtt
    36361 ttatccggtc aggaacgtga tggcgtagcg ttagctgatc aggtaaaaag tatcgcctgg
    36421 cgggcaagag gagcaacgaa gaaaggaaca gttgccccag aggaattaca actcattaaa
    36481 gccaaaatta acgtactgat tgggtagtgt tactaactca cttctattct ggtcacaggt
    36541 ttccacctga cagacccgca attttcagga caattcaatg aatcaaatcg accgtttgct
    36601 cactattatg cagcgcctgc gcgatccgga aaacggctgc ccgtgggata aagagcagac
    36661 atttgccacc attgcgcctt acacccttga agaaacctac gaagtgctgg acgccatcgc
    36721 ccgtgaagat tttgacgatc ttcgcggtga actgggcgat ctgctattcc aggtggtgtt
    36781 ttacgcgcaa atggctcagg aagaagggcg ctttgacttt aatgatattt gcgctgctat
    36841 tagcgataaa ttagagcgtc gccatccgca tgtttttgct gatagttctg ccgaaaacag
    36901 tagtgaagtg cttgcccgtt gggagcaaat caaaaccgaa gagcgcgcgc agaaagcgca
    36961 gcattcggcg ctggacgata ttcctcgtag tttaccggct ttaatgcgtg cgcaaaaaat
    37021 ccagaaacgt tgcgccaacg ttggcttcga ttggacgacg cttggtccgg tagtcgataa
    37081 agtctacgaa gagatcgacg aggtgatgta cgaagcgcgg caggctgttg tcgaccaggc
    37141 taaactggag gaggaaatgg gggacctgct gtttgccacg gtcaatctgg ctcgtcattt
    37201 agggacaaaa gcggaaatcg cattgcaaaa agcgaacgaa aaatttgagc gtcgttttcg
    37261 cgaagtggag cgtattgttg ccgcgcgtgg actggaaatg acaggtgttg acctcgaaac
    37321 aatggaagaa gtctggcaac aggtaaaacg gcaggaaatt gatctctaag ggaattacgc
    37381 ggtcaagcgc catttgtgtc attttttaaa tgacaagcgc ttgatttgcg tcaaaaacat
    37441 ttaccccaaa ggggctattt tctcactcct gatttcaata gtgcgctggc gaagaggagg
    37501 gataatgaaa gtttgtggca caggtcatgt tcgggtatac tgctttcccg tcctggttat
    37561 tccatcgtct tttcaaccta acttctcagg ttcagcatga caacgaacta tatttttgtg
    37621 accggcgggg tcgtatcctc tctgggtaaa ggcattgccg cagcctccct cgcagccatt
    37681 cttgaagccc gtggcctcaa tgtgaccatc atgaaactgg atccgtacat caacgtcgat
    37741 ccaggtacta tgagcccaat ccaacacggg gaagtgttcg ttactgaaga cggcgctgaa
    37801 accgacctgg acctggggca ctacgagcgt ttcattcgta ccaaaatgag ccgccgcaac
    37861 aacttcacca cgggtcgtat ctactctgac gttctgcgta aagaacgccg cggtgactac
    37921 ctcggcgcaa ccgtgcaggt tattccgcac atcactaacg caatcaaaga gcgcgtgctg
    37981 gaaggtggcg aaggtcatga cgtagtactg gtagaaatcg gcggtacagt aggtgatatc
    38041 gaatccttgc cgttcctcga agcgattcgc cagatggctg ttgaaattgg ccgtgagcac
    38101 actctgttta tgcacctgac gctggtgccg tacatggcag cgtctggtga agtcaaaacc
    38161 aaaccgactc agcactctgt aaaagagctg ctctccatcg gtatccagcc tgacatcctg
    38221 atttgtcgtt cagatcgcgc tgttccggcg aacgaacgtg cgaagattgc attgttctgt
    38281 aatgttccgg aaaaagcggt tatttctctg aaagacgtcg attccatcta taaaattccg
    38341 ggcctgttga aatctcaggg gctggacgat tatatttgta aacgattcag cttaaactgc
    38401 ccggaagcga atctgtccga atgggaacag gttatcttcg aagaagcgaa cccggtaagt
    38461 gaagtcacca tcggtatggt cggcaagtac attgaactgc cggatgctta taaatcagtg
    38521 atcgaagcac tgaaacacgg tgggctgaag aatcgtgtca gcgtcaacat caaactgatc
    38581 gattcacaag atgttgaaac gcgcggcgtt gaaatcctta aaggtctgga cgcaatcctc
    38641 gtacctggcg gtttcggcta tcgtggcgta gaaggcatga ttacgaccgc gcgttttgcg
    38701 cgtgagaaca atattcctta tctgggcatt tgcctgggta tgcaggtggc gttaattgat
    38761 tacgctcgcc atgttgccaa catggagaac gccaactcta cggaatttgt gccagactgt
    38821 aagtacccgg ttgtggcgct gattaccgag tggcgcgatg aaaacggcaa cgttgaagtt
    38881 cgtagcgaga agagcgatct cggcggtacc atgcgtctcg gcgcacagca gtgccagttg
    38941 gttgacgata gcctggttcg ccagctgtac aatgcgccga caattgttga gcgtcatcgt
    39001 caccgttacg aagtcaacaa catgctgttg aaacagattg aagatgcagg tctgcgcgtt
    39061 gcgggccgtt ccggggatga tcagttggtc gagatcatcg aagttccgaa tcacccgtgg
    39121 ttcgtggctt gccagttcca tccggagttt acttctactc cacgtgatgg tcacccgctg
    39181 tttgcaggct ttgtgaaagc cgccagcgag ttccagaaac gtcaggcgaa gtaagtaaaa
    39241 aagttagaac ggcaacgcgt accctgggta cgcgttgttt gtctggagtt tcagtttaac
    39301 tagtgacttg aggaaaacct aatgtccaaa atcgtaaaaa tcatcggtcg tgaaatcatc
    39361 gactcccgtg gtaacccgac tgttgaagcc gaagtacatc tggagggtgg tttcgtcggt
    39421 atggcagctg ctccgtcagg tgcttctact ggttcccgtg aagctctgga actgcgcgat
    39481 ggcgacaaat cccgtttcct gggtaaaggc gtaaccaaag ctgttgctgc ggtaaacggc
    39541 ccgatcgctc aggcgctgat tggcaaagat gctaaagatc aggctggcat tgacaagatc
    39601 atgatcgacc tggacggcac cgaaaacaaa tccaaattcg gcgcgaacgc aatcctggct
    39661 gtatctctgg ctaacgccaa agctgctgca gctgctaaag gtatgccgct gtacgagcac
    39721 atcgctgaac tgaacggtac tccgggcaaa tactctatgc cggttccgat gatgaacatc
    39781 atcaacggtg gtgagcacgc tgacaacaac gttgatatcc aggaattcat gattcagccg
    39841 gttggcgcga aaactgtgaa agaagccatc cgcatgggtt ctgaagtttt ccatcacctg
    39901 gcaaaagttc tgaaagcgaa aggcatgaac actgcagttg gtgacgaagg tggctatgcg
    39961 ccgaacctgg gttccaacgc tgaagctctg gctgttatcg ctgaagctgt taaagctgct
    40021 ggttatgaac tgggcaaaga catcactctg gcgatggact gcgcagcttc tgaattctac
    40081 aaagacggta aatacgttct ggctggcgaa ggcaacaaag cgttcacctc tgaagaattc
    40141 actcacttcc tggaagaact gaccaaacag tacccgatcg tttctatcga agacggtctg
    40201 gacgaatctg actgggacgg tttcgcatac cagaccaaag ttctgggcga caaaatccag
    40261 ctggttggtg acgacctgtt cgtaaccaac accaagatcc tgaaagaagg tatcgaaaaa
    40321 ggtatcgcta actccatcct gatcaaattc aaccagatcg gttctctgac cgaaactctg
    40381 gctgcaatca agatggcgaa agatgctggc tacactgcag ttatctctca ccgttctggc
    40441 gaaactgaag acgctaccat cgctgacctg gctgttggta ctgctgcagg ccagatcaaa
    40501 actggttcta tgagccgttc tgaccgtgtt gctaaataca accagctgat tcgtatcgaa
    40561 gaagctctgg gcgaaaaagc accgtacaac ggtcgtaaag agatcaaagg ccaggcataa
    40621 gactgacttt atctgattta aaaatgccag cctccgggct ggcatttttt atggttgagt
    40681 taccagcggc cggatgcgcc gccacctcca gaagaaccgc caccgccgct aaatccgccg
    40741 ccacttgagc ctccaccgga accgctgttg tctgaatgat aacttttacc tgatgctctt
    40801 ttctgaagta cacataaaca ggtaaagacc ataaatatgg tgaaagtaaa aaagaaggaa
    40861 aatacagcaa tggaaaaaat tttatcggag aacaggaata aatctaaaag atagattgct
    40921 gaaatataga ctccactttt acatgcgcga caaaaattac tgccttgatg aaaaaaccag
    40981 aaagggaaga acatcactgc aaatacccaa aaaatagcaa agtaataatg atcactggaa
    41041 gacgctgatt cactttctga aggattagtc ggatattggt gttgtgaagt gagttggtta
    41101 ttcaaagcgt ttatagctag ctctaatccc tgagcaaatt tttgttgttt aaatgcgggt
    41161 atcatgttgc tacggatgat atctccagcc agagcatcgg ttaccttttc ttccagccca
    41221 taacctacct ggatgcggac agtacgatct gaccaggcaa caatgatcag tatcccatca
    41281 ttacgtttgg catctcctaa acgccaattg tcaaaaactc ttgtagcata ttgttcaatt
    41341 gtctcgtctt tggttgtggg aacaattaat acagcaattt gcacttgggt attattgaaa
    41401 atagattcta acttgtgcgt cagttctttc ttttcttgtg agtttaagct actcgtaaga
    41461 tccgtaactt gttgctgtaa ttgtgggaca atagttggtt gggctgcaac aaaggaacac
    41521 acgaaagtga acattagtat gaaatagcgc
//
LOCUS       DAZGOB010000032        41397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGOB010000032 DAZGOB010000000
VERSION     DAZGOB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.032"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(312..524)
                     /gene="rpmE"
                     /locus_tag="ACHLIF_001747"
     CDS             complement(312..524)
                     /gene="rpmE"
                     /locus_tag="ACHLIF_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO9605780.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            727..2925
                     /gene="priA"
                     /locus_tag="ACHLIF_001748"
     CDS             727..2925
                     /gene="priA"
                     /locus_tag="ACHLIF_001748"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO9605781.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3081..4106
                     /gene="cytR"
                     /locus_tag="ACHLIF_001749"
     CDS             3081..4106
                     /gene="cytR"
                     /locus_tag="ACHLIF_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO9605782.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4198..5157
                     /gene="ftsN"
                     /locus_tag="ACHLIF_001750"
     CDS             4198..5157
                     /gene="ftsN"
                     /locus_tag="ACHLIF_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO9605783.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5250..5780
                     /gene="hslV"
                     /locus_tag="ACHLIF_001751"
     CDS             5250..5780
                     /gene="hslV"
                     /locus_tag="ACHLIF_001751"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO9605784.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5790..7121
                     /gene="hslU"
                     /locus_tag="ACHLIF_001752"
     CDS             5790..7121
                     /gene="hslU"
                     /locus_tag="ACHLIF_001752"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO9605785.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7188..8114
                     /gene="menA"
                     /locus_tag="ACHLIF_001753"
     CDS             7188..8114
                     /gene="menA"
                     /locus_tag="ACHLIF_001753"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO9605786.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8207..8692
                     /gene="rraA"
                     /locus_tag="ACHLIF_001754"
     CDS             8207..8692
                     /gene="rraA"
                     /locus_tag="ACHLIF_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO9605787.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="ACHLIF_001755"
     CDS             complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="ACHLIF_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO9605788.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9447..10292
                     /gene="glpF"
                     /locus_tag="ACHLIF_001756"
     CDS             9447..10292
                     /gene="glpF"
                     /locus_tag="ACHLIF_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO9605789.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10315..11823
                     /gene="glpK"
                     /locus_tag="ACHLIF_001757"
     CDS             10315..11823
                     /gene="glpK"
                     /locus_tag="ACHLIF_001757"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO9605790.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11958..12968
                     /gene="glpX"
                     /locus_tag="ACHLIF_001758"
     CDS             11958..12968
                     /gene="glpX"
                     /locus_tag="ACHLIF_001758"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO9605791.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13065..13811
                     /gene="fpr"
                     /locus_tag="ACHLIF_001759"
     CDS             13065..13811
                     /gene="fpr"
                     /locus_tag="ACHLIF_001759"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO9605792.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13816..14244)
                     /gene="uspD"
                     /locus_tag="ACHLIF_001760"
     CDS             complement(13816..14244)
                     /gene="uspD"
                     /locus_tag="ACHLIF_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO9605793.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14271..14570)
                     /gene="yiiS"
                     /locus_tag="ACHLIF_001761"
     CDS             complement(14271..14570)
                     /gene="yiiS"
                     /locus_tag="ACHLIF_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO9605794.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14782..15222)
                     /gene="yiiR"
                     /locus_tag="ACHLIF_001762"
     CDS             complement(14782..15222)
                     /gene="yiiR"
                     /locus_tag="ACHLIF_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9605795.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15323..15922
                     /gene="yiiQ"
                     /locus_tag="ACHLIF_001763"
     CDS             15323..15922
                     /gene="yiiQ"
                     /locus_tag="ACHLIF_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO9605796.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16030..16797
                     /gene="tpiA"
                     /locus_tag="ACHLIF_001764"
     CDS             16030..16797
                     /gene="tpiA"
                     /locus_tag="ACHLIF_001764"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO9605797.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16852..17607)
                     /gene="cdh"
                     /locus_tag="ACHLIF_001765"
     CDS             complement(16852..17607)
                     /gene="cdh"
                     /locus_tag="ACHLIF_001765"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO9605798.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17714..18703)
                     /gene="sbp"
                     /locus_tag="ACHLIF_001766"
     CDS             complement(17714..18703)
                     /gene="sbp"
                     /locus_tag="ACHLIF_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO9605799.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLV
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19022..19984)
                     /gene="pfkA"
                     /locus_tag="ACHLIF_001767"
     CDS             complement(19022..19984)
                     /gene="pfkA"
                     /locus_tag="ACHLIF_001767"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO9605800.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20165..21067)
                     /gene="fieF"
                     /locus_tag="ACHLIF_001768"
     CDS             complement(20165..21067)
                     /gene="fieF"
                     /locus_tag="ACHLIF_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO9605801.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21216..21716)
                     /gene="cpxP"
                     /locus_tag="ACHLIF_001769"
     CDS             complement(21216..21716)
                     /gene="cpxP"
                     /locus_tag="ACHLIF_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO9605802.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21866..22564
                     /gene="cpxR"
                     /locus_tag="ACHLIF_001770"
     CDS             21866..22564
                     /gene="cpxR"
                     /locus_tag="ACHLIF_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO9605803.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22561..23934
                     /gene="cpxA"
                     /locus_tag="ACHLIF_001771"
     CDS             22561..23934
                     /gene="cpxA"
                     /locus_tag="ACHLIF_001771"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO9605804.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24076..24750)
                     /gene="yiiM"
                     /locus_tag="ACHLIF_001772"
     CDS             complement(24076..24750)
                     /gene="yiiM"
                     /locus_tag="ACHLIF_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO9605805.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24899..25882)
                     /gene="kdgT"
                     /locus_tag="ACHLIF_001773"
     CDS             complement(24899..25882)
                     /gene="kdgT"
                     /locus_tag="ACHLIF_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO9605806.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26142..26762)
                     /gene="sodA"
                     /locus_tag="ACHLIF_001774"
     CDS             complement(26142..26762)
                     /gene="sodA"
                     /locus_tag="ACHLIF_001774"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO9605807.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27047..28081
                     /gene="rhaT"
                     /locus_tag="ACHLIF_001775"
     CDS             27047..28081
                     /gene="rhaT"
                     /locus_tag="ACHLIF_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO9605808.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWSNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28078..28926)
                     /gene="rhaR"
                     /locus_tag="ACHLIF_001776"
     CDS             complement(28078..28926)
                     /gene="rhaR"
                     /locus_tag="ACHLIF_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO9605809.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQHVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLAMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29000..29837)
                     /gene="rhaS"
                     /locus_tag="ACHLIF_001777"
                     /pseudo
     CDS             complement(29000..29837)
                     /gene="rhaS"
                     /locus_tag="ACHLIF_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            30125..31594
                     /gene="rhaB"
                     /locus_tag="ACHLIF_001778"
     CDS             30125..31594
                     /gene="rhaB"
                     /locus_tag="ACHLIF_001778"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO9605810.1"
                     /translation="MTFRNCVAVNLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDNF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31591..32850
                     /gene="rhaA"
                     /locus_tag="ACHLIF_001779"
     CDS             31591..32850
                     /gene="rhaA"
                     /locus_tag="ACHLIF_001779"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO9605811.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARQALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32933..33757
                     /gene="rhaD"
                     /locus_tag="ACHLIF_001780"
     CDS             32933..33757
                     /gene="rhaD"
                     /locus_tag="ACHLIF_001780"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO9605812.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAEVLVKIYSMGGMKQTITREELIALGERFGVTPLASALAL"
     gene            33829..34977
                     /gene="fucO"
                     /locus_tag="ACHLIF_001781"
     CDS             33829..34977
                     /gene="fucO"
                     /locus_tag="ACHLIF_001781"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO9605813.1"
                     /translation="MSFMLALPKISLHGAGAIGDMVNLVAHKQWGKALIVTDGQLVKL
                     GLLDSLFIALDQYEMSYHLFDEVFPNPTEELVQKGYAAFQDAACDYIIAFGGGSPIDT
                     AKAVKILTANPAPSTAYSGVGKVTNPGVPLVAINTTAGTAAEMTSNAVIIDTARQVKE
                     VIIDPNIIPDIAVDDASVMLEIPASVTAATGMDALTHAVEAYVSVGAHPLTDANALEA
                     IRLITLWLPKAVDDGHNLEAREQMAFAQYLAGMAFNSAGLGLVHALAHQPGATHNLPH
                     GVCNAILLPVIENFNRPNAIARFARIAQAMGVDTRDMSEEAASTEAINAIRALSHRVG
                     IPAGFSQLGITKEDIEGWLDKALADPCVPCNPRTASRDEVRELYLEAL"
     gene            34974..35288
                     /gene="rhaM"
                     /locus_tag="ACHLIF_001782"
     CDS             34974..35288
                     /gene="rhaM"
                     /locus_tag="ACHLIF_001782"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO9605814.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            35601..36047
                     /gene="frvA"
                     /locus_tag="ACHLIF_001783"
     CDS             35601..36047
                     /gene="frvA"
                     /locus_tag="ACHLIF_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO9605815.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            36058..37509
                     /gene="frvB"
                     /locus_tag="ACHLIF_001784"
     CDS             36058..37509
                     /gene="frvB"
                     /locus_tag="ACHLIF_001784"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO9605816.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            37499..38569
                     /gene="frvX"
                     /locus_tag="ACHLIF_001785"
     CDS             37499..38569
                     /gene="frvX"
                     /locus_tag="ACHLIF_001785"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9605817.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPQSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAKH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVSAFSQFRQVD"
     gene            38569..40317
                     /gene="frvR"
                     /locus_tag="ACHLIF_001786"
     CDS             38569..40317
                     /gene="frvR"
                     /locus_tag="ACHLIF_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO9605818.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(40367..>41397)
                     /gene="yiiG"
                     /locus_tag="ACHLIF_001787"
     CDS             complement(40367..>41397)
                     /gene="yiiG"
                     /locus_tag="ACHLIF_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO9605819.1"
                     /translation="LLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAPV
                     AKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPT
                     GEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEM
                     DKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIE
                     EREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMN
                     FSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEM
                     VDDYNSLR"
BASE COUNT        10060 a        10704 c        10711 g         9922 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggcaccaa tgcgcacccc ggctgaaccc tggacaatgc tactgccata ctgcgtcatg
      121 tcgccctgac gcgctgccgg ttttccgctc atcgtaaaat ccttttgaaa taaataagaa
      181 ccagaagcct agcgccagtc aacaacagga aatgataatg agttaacacc ccggaatttt
      241 tcagaattta taaaaggcaa attacaggca caaaaaaagc gccgtgcggc gcttttttcg
      301 gaaatccggt cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg
      361 ccaccggtag caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg
      421 tcgaggttca ggtcatgacc aacggtggag cggattttca ttacgttacc gcaagagcag
      481 ctagcagtaa tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt
      541 aaggccgcgt cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct
      601 tcaatacgat catttcgtac gaaggcgcga aatcatacag aaattaacca gcgtatgcaa
      661 actgatccgc actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat
      721 gatgctatgc ccgtagccca cgttgccttg cccgttccgc ttcctcgtac ctttgactat
      781 ctgctgccag aaggcatgac ggttaaagct gggtgtcgcg tgcgcgtgcc gtttggcaaa
      841 cagcaggagc gcatcgggat tgtggtatca gttagcgatg ccagcgaact gccgctcaat
      901 gagctaaaag cggtagtcga agtgctggac gttgagccgg tatttactca ctccgtctgg
      961 cggttactgc tctgggcggc agattactat catcatccga ttggcgatgt gctgtttcat
     1021 gccttgccga ttttactgcg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg
     1081 tttgccactg aacaaggtca ggcggtggat cttaacagcc tgaaacgttc gccaaagcaa
     1141 caacaggcgc tggcggcgtt acggcaaggc aaaatctggc gcgaccaggt cgcggaactt
     1201 gaatttaatg atgccgcgtt gcaggcgctg cgcaaaaaag gtctgtgtga tttagcaagt
     1261 gaaacaccag agtttagcga ctggcgaacg aactatgccg tttctggtga gcggttgcga
     1321 ttgaataccg aacaagccac cgccgttggc gcaattcata gcgcggcaga tactttttct
     1381 gcctggctgc tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg
     1441 gaaaacgtgc tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgaca
     1501 ccgcaaacta tcgcccgttt tcgtgaacgt tttaatgccc ccgtggaagt tctgcattcc
     1561 ggcctgaacg acagcgagcg gctttcggcg tggttgaaag cgaaaaatgg cgaggcggcg
     1621 attgtgatcg gcacccgctc cgcgctgttt acgccgttta aaaatctcgg cgtgattgtc
     1681 attgatgaag agcacgacag ctcctacaag cagcaggaag gctggcgcta tcatgcccgc
     1741 gacctggcgg tgtatcgtgc gcatagcgag caaatcccga ttattcttgg ctccgcaacg
     1801 cccgcgctgg aaacgttatg caacgtccag cagaaaaaat accgcctgct gcgcctgacc
     1861 cgtcgggcag ggaatgcgcg tccggcaatt caacatgtgc tggatttaaa aggtcagaag
     1921 gtgcaggcag gtctggctcc ggcgttaatc acccgtatgc gccagcattt acaggccaac
     1981 aatcaggtta tcctctttct taatcgtcgg ggttttgcgc ctgcgctgct gtgccacgac
     2041 tgcggctgga tagccgaatg cccacgttgc gatcactact acacgctgca tcaggcgcaa
     2101 cagcatttgc gctgtcacca ctgtgacagt cagcgtccgg tgccgcgcca gtgcccttcc
     2161 tgcggttcca cgcacctggt ccccgtgggg ctgggcaccg aacagcttga acagacgctt
     2221 gcgccgatgt tccccggcgt gcccatttca cgtatcgacc gcgataccac cagccgcaaa
     2281 ggggcgctgg aacagcaact ggcggaagtg catcgcggcg gcgcgaggat cttgattggc
     2341 acgcaaatgc tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat
     2401 gtggacggcg cgctgttttc tgccgatttt cgctcggcag aacgtttcgc ccagctttac
     2461 acccaggtcg ccggtcgtgc cgggcgtgcg ggtaaacagg gcgaagtggt gctgcaaacg
     2521 caccatccgg aacatcctct gttgcaaacg ttgctctata aaggctacga cgcctttgcc
     2581 gaacaggcgc tggctgagcg acgcatgatg cagctgccgc cgtggaccag ccatgtgatt
     2641 gtgcgtgcgg aagatcataa caatcagcat gcgccgctgt tcctgcaaca actgcgtaat
     2701 ctgatccttt ccagcccgct ggcagacgac aaactgtggg ttctcggccc ggttccggct
     2761 ctggcaccta agcgtggtgg ccgctggcgc tggcagatct tgttgcagca cccttcccgc
     2821 gtgcgcttgc aacacatcat tagtggtacg ctggcgctta ttaatacaat accggattcc
     2881 cgcaaggtga aatgggtgtt ggatgtcgat cccatcgaag gctaatgcgc aaacgttgcg
     2941 aggcggatcg aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc
     3001 acgcgctatc tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac
     3061 gtgccaggcg aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag
     3121 acgttgccct caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg
     3181 ataaagtctc ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt
     3241 taccgcagcc tatggggcgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg
     3301 tcccggatat ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg
     3361 caaatcacgg atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa
     3421 cctttatcga tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggttcaaggc
     3481 tgccgtttga tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg
     3541 aattcgcacc ggaactggag ctacctacag ttcatatcga caatctgacc gccgcatttg
     3601 atgcagtaaa ttatctatat gagcaagggc ataaacggat tggctgtata gccggtcccg
     3661 aagagatgcc gctgtgtcac taccgcctgc aaggttacgt tcaggcgctg cgtcgctgcg
     3721 gcattatggt tgatccgcaa tacatcgccc gtggcgactt caccttcgaa gccggaagca
     3781 aagcgatgca gcagctgctt gatcttccac aaccgcctac tgctgtcttc tgccatagcg
     3841 atgtgatggc gctcggcgca ctttctcagg caaaacgcca ggggctgaaa gtcccggaag
     3901 acctttccat aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga
     3961 caaccatcgc gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc
     4021 aaatgcaggg gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc
     4081 ggggatcaac acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag
     4141 gcccgccgct taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg
     4201 gcacaacgag attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc
     4261 tcacggaaaa agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc
     4321 gccgttcttg tgacctttat cggtggtctg tacttcatta cgcatcacaa gaaagaagag
     4381 tccgagacgc tgcaaagcca gaaagtgacc ggtaacggac taccaccaaa accagaagaa
     4441 cgctggcgct acattaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa
     4501 ccttctgccg gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgtcagctt
     4561 cttgaacaaa tgcaggctga tatgcgccag cagccaacgc agctggttga agttccgtgg
     4621 aatgaacaga cgccggaaca gcgtcagcaa acgctacaac gccagcgtca ggcgcagcaa
     4681 ttagcggagc agcaacgcct ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag
     4741 cagacgcgta cgtcgcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccggct
     4801 tctactcagc agccgtacca ggatctgctg caaacccctg cgcacacgac cgcgcagtcg
     4861 aagccgcagc aagctgcgcc ggttgctcgt gccgctgacg cgccaaaacc gacggcggag
     4921 aaaaaagacg aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca
     4981 gagacggtac gtgctcagct ggcgttcgaa ggatttgact cgaaaatcac caccaacaat
     5041 ggctggaatc gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc
     5101 aatcggttga agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac
     5161 cctcaaaatc ccccccatct ataattgcat tatgccccgt acttttgtac ggggtttgta
     5221 ctctgtattc gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg
     5281 gccatgtggt catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca
     5341 acgtgaaaaa ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta
     5401 ctgcggatgc ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc
     5461 atctggtcaa agccgccgtt gagctagcga aagactggcg taccgatcgc atgctgcgca
     5521 aacttgaagc actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg
     5581 gtgacgtggt gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc
     5641 aggctgcggc gcgtgcactg ttagaaaata ctgaactgag tgctcgtgaa attgctgaaa
     5701 aggcgttgga tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag
     5761 aattaagcta caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca
     5821 gcgaactgga taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc
     5881 tgcgtaaccg ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga
     5941 aaaatatcct gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg
     6001 ctaagctggc gaatgcgccg ttcatcaaag ttgaagcgac caaattcacc gaagtaggct
     6061 acgtcggtaa ggaagtggat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg
     6121 tacgcgtcca ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta
     6181 ttctcgacgt gctgatccca cctgctaaaa acaactgggg acagaccgaa cagcagcagg
     6241 aaccgtccgc tgctcgtcag gcattccgca aaaaactgcg tgaaggccag cttgatgaca
     6301 aagaaatcga gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccag
     6361 gcatggaaga gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc
     6421 aaaaagcgcg taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg
     6481 cgaaactggt gaacccggaa gagctgaagc aagacgctat cgacgctgtt gagcagcacg
     6541 ggatcgtgtt tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg
     6601 atgtttctcg tgaaggcgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt
     6661 ccaccaaaca cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc
     6721 agattgcgaa accgtctgac ctaatcccgg aactgcaagg tcgtctgcca atccgcgttg
     6781 aactgcaggc actgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta
     6841 tcaccgtgca gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact
     6901 ccggtattaa gcgcatcgcg gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg
     6961 gtgctcgtcg tttacacact gttctggagc gtttaatgga agagatttcc tacgacgcca
     7021 gcgatttaag cggtcaaaat atcactattg acgcagatta tgtgagcaaa catctggatg
     7081 cgttggtggc agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca
     7141 tcattgtttg atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac
     7201 aaattagccg aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct
     7261 ttgctgcaat tatcgtcggg acggcgctgg catggtggca aggtcacttc gatccgctgg
     7321 tcgccctgct ggcactaatt accgccgggc tattacagat cctttctaac ctcgccaatg
     7381 attacggcga tgcggtaaaa ggcagcgata aacctgaccg cattgggccg ctacgcggca
     7441 tgcaaaaagg ggtcattacc cagcaagaga tgaaacgggc gctcattatt accgtcgtgc
     7501 tcatctgtct ctccgggttg gcactggttg cagtggcatg ccatacgctg gccgattttg
     7561 tcggtttcct gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca
     7621 atcgtcctta tggttatatc ggtctgggtg atatttccgt actggttttc tttggctggt
     7681 tgagtgtcat ggggagctgg tatttacagg ctcatacatt gattccggca ctgatccttc
     7741 cggcgaccgc atgcggcctg ctggcaacgg cagtactgaa tattaataac ctgcgtgata
     7801 tcaatagcga ccgcgaaaat ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg
     7861 cgcgtcgtta tcatgcctgc ctgctgatgg gctcgctggt gtgtctggcg ctgtttaatc
     7921 tcttttcgct gcatagcctg tggggctggc tgttcctgct ggcggcacca ttactggtga
     7981 agcaagcccg ttatgtgatg cgggaaatgg acccggtggc gatgcgacca atgctggaac
     8041 gtactgtcaa gggagcgtta ctgactaacc tgctgtttgt tttagggata ttcctaagcc
     8101 agtgggcagc ataactgaca aatatcaatt aacaattgat gattttgcca acagcccaca
     8161 tagcgcgata tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac
     8221 ttccgagctt tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa
     8281 ctttggcgga cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa
     8341 cgggttgctg tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg
     8401 cggtggttct gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa
     8461 tgaatgggaa ggtctggtca tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt
     8521 ggatatcggc atccaggcga tggcggcaat tccggttggt gccgctggcg aaggcattgg
     8581 cgaaagcgat gtccgcgtca attttggcgg tgtcaccttc ttctccggcg accatcttta
     8641 tgccgacaat accgggatta ttctttctga agatccgctg gatattgaat gatagaaaag
     8701 gcaccgcaag gtgccttttt tctgcgttac ctgttggcct acacagtaaa gaaattacgc
     8761 ggaagatgaa gcgtaatcag acctcttcca tgcgacccag cagggcctgc agacgttcct
     8821 gccagccgtt ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat
     8881 gctgggcatt ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt
     8941 ccatctgcaa cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa
     9001 acacttctaa tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaatc
     9061 ctcgtcccga ttaccggtga cgccttaata aatacgagcg cactttagtt agctccgatt
     9121 gtatgaagcc gcgccatcgc tgtccagcgg cacgccttgc agattacggt ttgccacact
     9181 tttcatcctt ctcctggtga cataatccac atcaatcgaa aatgttaata aatttgttgc
     9241 gcgaatgatc taacaaacat gcatcatgta caatcagatg gaataaatgg cgcgataacg
     9301 ctcattttat gacgaagcac acacatttta agttcgatat ttctcgcttt tgctcgttaa
     9361 cgataagttt acagcatgcc tacaagcatc gtggaggtcc gtgactttca cgcatacaac
     9421 aaacattaac tcttcaggat ccgattatga gtcaaacatc aaccttgaaa ggccagtgca
     9481 ttgctgaatt cctcggtacc gggttgttga ttttcttcgg tgtgggttgc gttgcagcac
     9541 taaaagtcgc tggtgcgtct tttggtcagt gggaaatcag tgtcatttgg ggactggggg
     9601 tggcaatggc catctacctg accgcagggg tttccggcgc gcatcttaat cccgctgtta
     9661 ccattgcatt gtggctgttt gcctgtttcg acaagcgcaa agttattcct tttatcgttt
     9721 cacaagttgc cggcgctttc tgtgctgcgg ctttagttta cgggctttac tacaatttat
     9781 ttttcgactt cgagcagact catcacattg ttcgcggcag cgttgaaagt gttgatctgg
     9841 ctggcacttt ctctacttac cctaatcctc atatcaattt tgtgcaggct ttcgcagttg
     9901 agatggtgat taccgctatt ctgatggggc tgatcctggc gttaacggat gatggcaacg
     9961 gtgtaccacg cggccctttg gctcccttgc tgattggtct actgattgcg gtcattggcg
    10021 catctatggg cccattgaca ggttttgcca tgaacccagc gcgtgacttc ggtccgaaag
    10081 tctttgcctg gctggcgggc tggggcaatg tcgcctttac cggcggcaga gacattcctt
    10141 acttcctggt gccgcttttc ggccctatcg ttggcgcgat tgtaggtgca tttgcctacc
    10201 gcaaactgat tggtcgccat ttgccttgcg atatctgtgt tgtggaagaa aaggaaacca
    10261 caactccttc agaacaaaaa gcttcgctgt aatatgacta cgggacaatt aaacatgact
    10321 gaaaaaaaat atatcgttgc gctcgaccag ggcaccacca gctcccgcgc ggtcgtaatg
    10381 gatcacgatg ccaatatcat tagcgtgtcg cagcgcgaat ttgagcaaat ctacccaaaa
    10441 ccaggttggg tagaacacga cccaatggaa atctgggcca cccaaagctc cacgctggta
    10501 gaagtgctgg cgaaagccga tatcagttcc gatcaaattg cagctatcgg tattacgaac
    10561 cagcgtgaaa ccactattgt ctgggaaaaa gaaaccggca agcctatcta taacgccatt
    10621 gtctggcagt gccgtcgtac cgcagaaatc tgcgagcatt taaaacgtga cggtttagaa
    10681 gattatatcc gcagcaatac cggtctggtg attgacccgt acttttctgg caccaaagtg
    10741 aagtggatcc tcgaccatgt ggaaggctct cgcgagcgtg cacgtcgtgg tgaattgctg
    10801 tttggtacgg ttgatacgtg gcttatctgg aaaatgactc agggccgtgt ccatgtgacc
    10861 gattacacca acgcctctcg taccatgttg ttcaacatcc ataccctgga ctgggacgac
    10921 aaaatgctgg aagtgctgga tattccgcgc gagatgctgc cagaagtgcg tcgttcttcc
    10981 gaagtatacg gtcagactaa cattggcggc aaaggcggca cgcgtattcc aatctccggg
    11041 atcgccggtg accagcaggc cgcgctgttt ggtcagttgt gcgtgaaaga agggatggcg
    11101 aagaacacct atggcactgg ctgctttatg ctgatgaaca ctggcgagaa agcggtgaaa
    11161 tcagaaaacg gcctgctgac caccatcgcc tgcggcccga ctggcgaagt gaactatgcg
    11221 ttggaaggtg cggtgtttat ggcaggcgca tccattcagt ggctgcgcga tgagatgaag
    11281 ttgattaacg acgcctacga ttccgaatat ttcgccacca aagtgcaaaa caccaatggt
    11341 gtgtatgtgg ttccggcatt taccgggctg ggtgcgccgt actgggaccc gtatgcgcgc
    11401 ggggcgattt tcggtctgac tcgtggggtg aacgctaacc acattatacg cgcgacgctg
    11461 gagtctattg cttatcagac gcgtgacgtg ctggaagcga tgcaggccga ctctggtatc
    11521 cgtctgcacg ccctgcgcgt ggatggtggc gcagtagcaa acaatttcct gatgcagttc
    11581 cagtccgata ttctcggcac ccgcgttgag cgcccggaag tgcgcgaagt caccgcattg
    11641 ggtgcggcct atctcgcagg cctggcggtt ggcttctggc agaacctcga cgagctgcaa
    11701 gagaaagcgg tgattgagcg cgagttccgt ccaggcatcg aaaccactga gcgtaattac
    11761 cgttacgcag gctggaaaaa agcggttaaa cgcgcgatgg cgtgggaaga acacgacgaa
    11821 taatgtaaat gccgaatgaa gcgtttatgc cgcatccggt agtcccgaaa cgtgcggggg
    11881 caaccccgca cacatcaata atccctccct tcccctgtgc tacacttcgc gccattcctt
    11941 actgcttaga gtttgctatg agacgagaac ttgccatcga attttcccgc gtcaccgaat
    12001 cagcggcgct ggctggctac aaatggttag gacgcggcga taaaaacacc gcggacggcg
    12061 cggcggtaaa cgccatgcgt attatgctca accaggtcaa cattgacggc accatcgtca
    12121 ttggtgaagg tgaaatcgac gaagcaccga tgctctacat tggtgaaaaa gtcggtactg
    12181 gtcgcggcga cgcggtagat attgctgttg atccgattga aggcacgcgc atgacggcga
    12241 tgggccaggc taacgcgctg gcggtgctgg cagtaggcga taaaggctgc ttcctcaatg
    12301 cgccggatat gtatatggag aagctgattg tcgggccggg agccaaaggc accattgatc
    12361 tgaacctgcc gctggcggat aacctgcgca atgtagcggc ggcgctcggc aaaccgttga
    12421 gcgaactgac ggtaacgatt ctggctaaac cacgccacga tgccgttatc gctgaaatgc
    12481 agcaactcgg cgtacgcgta tttgctattc cggatggcga cgttgcggcc tcaattctca
    12541 cctgtatgcc agacagcgaa gttgacgtgc tgtacggtat tggtggcgcg ccggaaggcg
    12601 tagtttctgc ggcggtgatc cgcgcattag atggcgacat gaacggtcgt ctgctggcgc
    12661 gtcatgacgt caaaggcgac aacgaagaga atcgtcgcat tggcgagcag gagctggcac
    12721 gctgcaaagc gatgggcatc gaagccggta aagtattgcg cctgggcgat atggcgcgca
    12781 gcgataacgt catcttctct gccaccggta ttaccaaagg cgatctgctg gaaggcatta
    12841 gccgcaaagg caatatcgcg actaccgaaa cgctgctgat ccgcggcaag tcacgcacca
    12901 ttcgccgcat tcagtccatc cactatctgg atcgcaaaga cccggaaatg caggtgcaca
    12961 tcctctgatt gatttgatcg attgagcctt ccagtccttc gggactggaa tttttttgtt
    13021 cggagaacga agataaggca agtcaatcaa aacaggagaa aaacatggct gattgggtaa
    13081 caggcaaagt cactaaagtg cagaactgga ccgacgccct gtttagtctc accgttcacg
    13141 cccccgtgct tccgtttacc gccgggcaat tcaccaagct tggccttgaa atcgacggcg
    13201 aacgcgtcca gcgcgcctac tcctatgtta actcgcccga taatcccgat ctggagtttt
    13261 acctggtcac cgtccccgat ggcaaattaa gcccacgatt ggcggcactg aaaccaggcg
    13321 atgaagtgca ggtggttagc gaagcagctg gcttctttgt tctggatgaa gtaccggact
    13381 gcgaaacgct atggatgctg gcaaccggta cagcgattgg cccttattta tcgattctgc
    13441 aactaggcaa agatttagat cgcttcaaaa atctggtcct ggttcacgcc gcacgttatg
    13501 ccgccgactt aagctatttg ccactgatgc aagaactgga aaaacgctac gaagggaaac
    13561 tgcgcattca gacggtggtc agtcgggaaa cggcagcggg gtcgctcacc gggcggatac
    13621 cggcgttaat tgaaagtggg gaactggaaa gcgcgattgg cctgccgatg aataaagaaa
    13681 ccagccatgt gatgctgtgc ggcaatccac agatggtgcg cgatacacaa cagttgctga
    13741 aagagacccg gcagatgacg aaacatttac gtcgccgacc gggccatatg acagcggagc
    13801 attactggta agcggttact tatcgataaa cggcacgatg agcaaatccg cactcatctt
    13861 attgatcatc ccgcgatatg ccggcatcaa acggttgata aatgagtgat gatgaccaca
    13921 gacaaggagg tcgcactgct ctttttgcat aatttccagc agtgtttccg gcatttctcc
    13981 gcgttcaata cgcagttttg tcttcggcca ttgaatattt ttcgtcagtt tatacagctt
    14041 gttatccgac ttattcttca acaattgaag aatatcttct gttgcaggga agtagatacc
    14101 cggatacaac tcgcttaagc catcatcaat atgaattaac gtcaggtgag cgtcattatg
    14161 tctggcgagc tccagagctt tattcaccag taaggcatct tcttcattcc cggaaattgc
    14221 cacgccaatg tgtttataag ccatgtttaa ctccttctaa agcaactcca tcaagctaac
    14281 gaacgcagtg ataactcaaa aataatcatc tcggcctggc atgagaaagt gaaggccgca
    14341 tcaagctcaa cttgcccctc cagatcctta atcttgtagg ccacgctggc cccttcatct
    14401 gcagaggatg cagcggcggc aagctctttc agtttggtca taaaagactc tgcttcttcg
    14461 cgggtggcaa aaaagcgcga aaacttactg gtacagttgt cgttatcaat caccgtaccg
    14521 atatcaatcg cacatccttt agtactgcat ttatctacga catctttcat agggcacctc
    14581 tgtatattcg cctctctgtc gattacgact taagaataat gcgccgttaa gggaagaaca
    14641 aacaagtaat gatcttatgg attaccattt tttttgatat tgcttaaggt gaagagttca
    14701 gaaggatttt ccgggtattg tcatttgcat cgtggaaata acaatcccgg aaaaaaatag
    14761 cagtaagaga tatattaaag gttaattact tgatttattg tcggctttat acttcacatc
    14821 ctgagtatct ttaccatatt tattttcgcc ttgtgtgcca acaaacgcac caagatcgat
    14881 aagcatcatc accagaatca acgtcgggac aaaacgcccc accgcccatt gccagacacc
    14941 cggtaaaatc gcccagttac ccgccagcag catccacgcc acaatcatca gaaatgccca
    15001 tgcgccggaa cgcccgcgat catgcaagcg cttaacagtt actgccgctg ttggccaaag
    15061 caagcacaca aggcaaaacg ccgcggtctg aatatcgagt aaatttttac ccgccagtga
    15121 gaaaagcacc agcatgcctg cgaaccacag gcctatccaa atccagaaat cacggcgtcc
    15181 aatacgccct ttaaatgaga ataaccattg ctgtatggtc atgtaagttc cttgatggtt
    15241 gtcttttcca ggattctacc cttttgacaa gggcgacaga tatcgtttta atcggagcca
    15301 gtcataacaa aaggtactgt caatgaagcc agggtgtacg ctgttttttc tcttatgttc
    15361 tgcattaacc gttacaacaa cggcgcatgc acaaacacca gatacggcaa cgaccgcgcc
    15421 ttatctgctg gctggagccc ctactttcga tctctccatc agccagtttc gagaagactt
    15481 taacagccag aatcctagcc tgccactgaa cgaatttcgt gccatcgaca gcagtcccga
    15541 caaagccaat ctcactcgtg ctgcaagtaa aattaacgag aacttgtatg cttctacagc
    15601 gctggagcgc ggtaccttaa aaatcaaaag cattcaaatg acctggctac ccatccaggg
    15661 gccagagcaa aaagcagcga aagcgaaagc tcaggaatac atggcagcag tgatccgcac
    15721 actcacccca ttaatgacca aaacacaaag ccagaaaaaa ctgcagtcgc tactaacggc
    15781 ggggaaaaac aaacgttatt acaccgagac agaaggtgca ctgcgttatg ttgtcgcgga
    15841 caacggcgaa aaggggctga ccttcgctgt tgaaccgatt aagctggcgc tatctgaatc
    15901 gcttgaaggt ttgaataaat gacaaaaagc aaagcctttg tgccgatgaa tctctatact
    15961 gtttcacaga cctgctgccc tgcggggcgg ccatcttcct ttattcgctt ataagcgtgg
    16021 agaattaaaa tgcgacatcc tttagtgatg ggtaactgga aactgaacgg cagccgccac
    16081 atggttcacg agctggtttc taacctgcgt aaagagctgg caggtgttgc tggctgtgcg
    16141 gttgcaatcg caccaccgga aatgtacatc gatatggcga agcgcgaagc tgaaggcagc
    16201 cacatcatgc tgggtgcgca aaacgtggac ctgaacctgt ccggcgcatt caccggtgaa
    16261 acttctgccg ctatgctgaa agacatcggc gctcagtaca tcatcatcgg ccactctgaa
    16321 cgtcgtactt accacaaaga gtctgacgaa ctgattgcga aaaaattcgc ggtgctgaaa
    16381 gagcagggcc tgactccggt tctgtgcatc ggtgaaaccg aagctgaaaa cgaagcgggc
    16441 aaaactgaag aagtttgcgc acgtcagatc gacgcggtac tgaaaactca gggtgctgcg
    16501 gcattcgaag gtgcggttat cgcttacgaa cctgtatggg caatcggtac tggcaaatct
    16561 gcaactccgg ctcaggcaca ggctgttcac aaattcatcc gtgaccacat cgctaaagtt
    16621 gacgctaaca tcgctgaaca agtgatcatt cagtacggcg gctctgtaaa cgcgtctaac
    16681 gctgcagaac tgtttgctca gccagatatc gacggcgcgc tggttggcgg tgcttctctg
    16741 aaagctgacg ctttcgcagt gatcgttaaa gctgcagaag cggctaaaca ggcttaagtc
    16801 tgacaggtgc cggatttcat atccggcact tactttcctt aactcctcgc cttaacgcaa
    16861 aatctcacac tcatgatcct ggatttcctc ggctgaagca cggttcagcg tcagtagatt
    16921 tcgttgtgtc gccagcaaca caaacgaatt atcactctgc cgtaccatcg ccagcccgta
    16981 gcttcccata tgttcccgcg cctcaggcac ttcttctgca agcatcataa atgggctgcg
    17041 ttgtaccagt tcgctttccg ttacccgacg cgccaggtat tcatgcccgc gcaaaccacc
    17101 tggcagtggc aaccagcggc tgctgatgtt cgccagattt ttatccagtt gttcgcgcac
    17161 atcaggacga atacaagaga tatgaatatg aaaatggttt tgcgtacgcc cggtgcggga
    17221 gttgatcgcc aaagaaaccg cgcgatcggg aaccggctgg ccgtattttt tgctcataaa
    17281 atcacgcgcc tgccaggcca accaaaagaa gttcggcgtt gaaggatcgg tcaacaaagg
    17341 actttcagta ccgttaatac gatacgttgg catcaacaga tattgcagtg gaccattaag
    17401 atcttttaaa accacgtatc cggcattggg tttgacttcc gcacatggcg aaggattttg
    17461 attttgctgc tgattgggca aacattcctc aaggacaatc ttacgtaatg tatccgactc
    17521 ttcaccggtt aatttccagt aaccaatacc ggcagccaca acggcgataa ctatcatcac
    17581 caaaaaaaga agacccgctt ttttcatctt tttttccctg tacctcaaag agatgcaagg
    17641 gtaacgcaaa atcgtgacaa ataaaaaacc cggtcgcgag aaaccaccgg gtttgataat
    17701 tatcctgggg aaatcagcgt ttgctgatct gatcgaacgt accgccgtta gcaaaatgct
    17761 ctttttgcgc tttcgtccag ccgccgaact cttcatcaat ggtgaataac ttcagctttg
    17821 gaaacgcatt ttcgtacttt ttcgctactt cagcgtcgcg cggacggtag tagtttttcg
    17881 cggcaatttc ctgaccttct ggcgagtaga gatatttcag gtaggcttcc gccacctctt
    17941 tggtgccttt tttctcgacc actttatcga ccaccgacac ggttggctct gcgaggatag
    18001 actcactcgg cgtgacgatt tcgaatttat ctttccccag ttcattcgct accagcagag
    18061 cttcgttttc ccaggcgatc agtacatcgc caatcccgcg ctcgacaaag gtgttagtgg
    18121 agccgcgcgc gccagaatcc agaacttcga cgtttttata cagtgcccga acaaaatcct
    18181 gtgcttttgc ctgatcgttg ttgttgtgat gcagcgcgta gccccaggca gccaggtagt
    18241 tccagcgtgc gccaccagag cttttcggat taggcgtgat caccgaaaca cccggtttaa
    18301 tcagatcgtt ccagtcatgg atctgcttcg gattgccctt acgcaccagg aaaacaatgg
    18361 tggaagtgta cggtgcggag ttatccggca gacgtttgat ccactcttta tcaatccgcc
    18421 cgcgttccgc aatcgcgtcc acgtcatagg ccagagccag cgtgacaaca tcagcttcaa
    18481 taccgttgat taccgacgtc gcttgtttac ctgaaccacc gtgtgactga cgaatcacca
    18541 cgttatcacc agtttgctgt ttccagtggg cgctgaatgc cttgttgtac tgttcgtaca
    18601 attcgcgcgt tggatcatat gaaacgttaa gaagctgaat atcctttgcc ataacgttgg
    18661 ttgccgccag caaaaatgtt aaccctacgc cccacttgtt catcgcccga ctctcttatg
    18721 ttgtgttgtg atgagcaaag cgtgccagaa ggttaaccaa acattaaaga ataaaaaaag
    18781 attggctata acttgcgggt atatgttggg ggttaaaaag gcggaagaaa tccgcctcat
    18841 attgctgaca aagtgcgctt tgtccatgcc ggatgcggcg cgaacgcctt atccggccta
    18901 caaaagtttg caaattcaat aaattgcaga attcatgtag gcctgataag cgaagcgcat
    18961 caggcatttt tgcttctgtc atcggtttca gggtaaagga atctgccttt ttccgaaatc
    19021 attaatacag ttttttcgcg cagtccagcc agtcaccttt gaacggacgc ttcatgtttt
    19081 cgatagcgtc gatgatgtcg tggtgaacca gctgttcgtt ctggatacct acacaacgac
    19141 cgccgtaacc tgccagcagc agatcgatag cgtaagcgcc catacgggaa gccagaatac
    19201 ggtcgtaagg caccggagaa ccaccgcgct ggatgtggcc cagcacagtt gcgcgggttt
    19261 cacgaccggt ttctttctcg atgaaatgcg ccagttcgtc aacatcacac atatgttcgg
    19321 taatcgccac gatcgcgtgt tttttacctt tcgcgatacc cgctttgatt tcgtttacca
    19381 ggtcttcacg gctgaattca acttccggaa ccacaacgaa ttcacagcca ccggcaatgg
    19441 ccgcagccaa cgtcagatcg ccacaataac ggcccatcac ttccaccacg gaaatacgct
    19501 ggtgagaaga agaggtgtca cgcagacggt cgatcgcttc tacaacggtg ctcagcgcag
    19561 tgaagaaacc gatagtgtag tcagtgcctt tgatgtcgtt gtcgatagtg cccggcagac
    19621 cgatgcacgg gaagcccatt tcggtcagac gcattgcacc catgtaggaa ccgtcaccgc
    19681 cgataaccac cagcgcgtcg atcccacgtt ttttcaggtt ttcgatagcc acggcgcgga
    19741 tgttctcgtc gcggaattcc gggaagcgcg cagaaccgag gaacgtaccg ccacggttga
    19801 tcatgtcaga aacgctgtaa cggtctagct gtaccatacg gtcttcatac agacccagat
    19861 agccgtcata aatgcccatt acttccagac cttctgtcag cgcagaacga acaaccccgc
    19921 gaattgcggc gttcatgcct ggcgcatcac cgccgcttgt caacacaccg attttcttaa
    19981 tcatgactac ctctgaactt tggaatgcaa aataaaatct gttgccggaa gtcttcttgc
    20041 acatcgaagt gatccaacga atgtgcaaat agtataacaa tcacttcctg ctgaattgat
    20101 tcaggtcagg ccaaatggcg gtattttata cacaaaatgc gggtctggct ctcttttata
    20161 ctgattatga aagcatagac cgtttaccct ccctgggtac gacggaacag gggtcctgat
    20221 ggataattac atccgatccc ggaaaacgcc gtaaaatagc ctgctctacc tgatccgcca
    20281 ccatatgtgc ctgaaccaaa ggcagagagt cttccatttc caaatgaatc tgaataaagc
    20341 gggtcggccc tgactgccgc gtgcgaagat cgtgagcgcc gctaacaccc ggccaggaag
    20401 tcacgatatc aataatttct tgccgttcct catcaggcaa tgcgcgatcc agtaatgact
    20461 gtaccgcctc atatcccatg cgtaacgcgc tatataaaat atagatgccg attcccaatg
    20521 caaacagagc atcggcgcga tgccagccgt accaggacaa ccccagcgcc agcagaattg
    20581 cgccgttcat cataacatca gactggtaat gtagcatatc agcccgcacc gcctggcttt
    20641 gcgtccggcg caccacccaa cgctgaaacg agacaaggat aatcgtacaa attagcgcca
    20701 caattgtcac gataaccccg acgcctggat ctgtcatcgg tgttggagat atcagatgtt
    20761 gaatacccgt caaaaacagg aatagtgccg aaccggagat aaacatactt tgcgccagcg
    20821 ccgcgaggga ctctgcttta ccgtgaccaa acgagtgatt atcgtcggca ggttgcaggg
    20881 aatatcgcac caccagtaaa ttcgtcaacg acgcgccgat atccaccagc gaatccacca
    20941 gcgcggcgag aatactcacc gacccggtat accaccatgc aaaaatttta atcagcaata
    21001 gcaacgaagc catcgccgtc gcagcaatcg ccgcccgact gaccagccgt ccataagatt
    21061 gattcataaa tactcccgct atcaactgac gctagtataa cggaagcaaa tcatctgcaa
    21121 tgcattaagc agcaggcaaa ttgaggataa aaaaaacccc cacatcatgt gggggaagac
    21181 agggatggtg tctatggcaa ggaaaacagg gtttactact gggaacgtga gttgctacta
    21241 ctcaatagct tcaacgatga acttttttgc cattgcgtca cgtcacgcaa ctgctccatt
    21301 cgttgttgat gtttctcgtt taaaaccgct tgctgctccg gcgttaacag gcgatacatt
    21361 tggttgcgga cttttgccat ctcaacctga cgagcaattt gctcattcgc cattttttct
    21421 gcctgtgcgc gcacagcgtt ttcatcaaaa ttttctgcgg tgacaaggcg atgcattgtc
    21481 tccagttcgc taacattaac aggaggctgt tcgtgccggg cctgttgcat aagatctcgc
    21541 atctgctgac gctgatgttc ggttaaactt atgccgtcga acatatggct ctgcgtactg
    21601 cgctgcgtaa gttcttcacc cggatgccag ttatcgcctg aaccgacttc agcagcgtgg
    21661 cttaatgaac tgactgccag cgttgaggcc atgacggcag cggtaactat gcgcatcatt
    21721 tgctcccaaa atctttctgt cgcgattcaa cgatagagag tttacgattc aggctgcaaa
    21781 catgcgtcag ggggtgtaaa acaacgtaaa gtcatggatt agcgacgtct gatgacgtaa
    21841 tttctgcctc ggaggtattt aaacaatgaa taaaatcctg ttagttgatg atgaccgaga
    21901 gctgacttcc ctattaaagg agctgctcga gatggaaggc ttcaacgtga ttgttgccca
    21961 cgatggggaa caggcgcttg atcttctgga cgacagcatt gatttacttt tgcttgacgt
    22021 aatgatgccg aagaaaaatg gtatcgacac attaaaagca cttcgccaga cacaccagac
    22081 gcctgtcatt atgttgacgg cgcgcggcag tgaacttgat cgcgttctcg gccttgagct
    22141 gggcgcagat gactatctcc cgaaaccgtt taatgatcgt gagctggtgg cacgtattcg
    22201 cgcgatcctg cgccgttcgc actggagcga gcaacagcaa aacaacgaca acggttcacc
    22261 gacactggaa gttgatgcct tagtgctgaa tccaggccgt caggaagcca gcttcgacgg
    22321 gcaaacgctg gagttaaccg gtactgagtt taccctgctc tatttgctgg cacagcatct
    22381 gggtcaggtg gtttcccgtg aacatttaag ccaggaagtg ttgggcaaac gcctgacgcc
    22441 tttcgaccgc gctattgata tgcacatttc caacctgcgt cgtaaactgc cggatcgtaa
    22501 agatggtcac ccgtggttta aaaccttgcg tggtcgcggc tatctgatgg tttctgcttc
    22561 atgataggca gcttaaccgc gcgcatcttc gccatcttct ggctgacgct ggcgctggtg
    22621 ttgatgttgg ttttgatgtt acccaagctc gattcacgcc agatgaccga gcttctggat
    22681 agcgaacagc gtcaggggct gatgattgag cagcatgtcg aagcggagct ggcgaacgat
    22741 ccgcccaacg atttaatgtg gtggcggcgt ctgttccggg cgattgataa gtgggcaccg
    22801 ccaggacagc gtttgttatt ggtgaccacc gaaggccgcg tgatcggcgc tgaacgcagc
    22861 gaaatgcaga tcattcgtaa ctttattggt caggccgata acgccgatca tccgcagaag
    22921 aaaaagtatg gccgcgtgga actggtcggt ccgttctccg tgcgtgatgg cgaagataat
    22981 taccaacttt atctgattcg tccggccagc agttctcaat ccgatttcat taacttactg
    23041 tttgaccgcc cgctattact gctgattgtc accatgttgg tcagtacgcc gctgctgttg
    23101 tggttggcct ggagtctggc aaaaccggcg cgtaagctga aaaacgctgc cgatgaagtt
    23161 gcccagggaa acttacgcca gcacccggaa ctggaagcgg ggccacagga attccttgcc
    23221 gcaggtgcca gttttaacca gatggtcacc gcgctggagc gcatgatgac ctctcagcag
    23281 cgtctgcttt ctgatatctc tcacgagctg cgcaccccgc tgacgcgtct gcaactgggt
    23341 acggcgttac tgcgccgtcg tagcggtgaa agcaaggaac tcgagcgtat tgaaaccgaa
    23401 gcgcaacgtc tggacagcat gatcaacgat ctgttggtga tgtcacgtaa tcagcaaaaa
    23461 aacgcgctgg ttagcgaaac catcaaagcc aaccagttgt ggagtgaagt gctggataac
    23521 gcggcgttcg aagccgagca aatgggcaag tcgttgacag ttaacttccc gcctgggccg
    23581 tggccgctgt acggcaatcc gaacgccctg gaaagtgcgc tggaaaacat tgttcgtaat
    23641 gctctgcgtt attcccatac gaagattgaa gtgggctttg cggtagataa agacggtatc
    23701 accattacgg tggacgacga tggtcctggc gttagcccgg aagatcgcga acagattttc
    23761 cgtccgttct atcggaccga tgaagcacgc gatcgtgaat ctggcggtac aggattgggg
    23821 ctggcgattg ttgaaaccgc cattcagcag catcgtggct gggtgaaggc agaagacagc
    23881 ccgctgggcg gtttacggct ggtgatttgg ttgccgctgt ataagcggag ttaaactccg
    23941 catttgtagg caggataagg cgtttacgcc gcatccggca tttgagcagg ataaggcgtt
    24001 tacgccgcat ccggcatttg agcaggatgc ctgatgcgac gctgatagcg tcttatcagg
    24061 cctacactcc gggggttatt ttccccacaa ccggcgggaa aaatcttcga tcttgccgct
    24121 cagtcggcgc ttttgcatcg tccgcgtcca gcttttcgat aacccggcgg cggagagtaa
    24181 acggtgatac tgatcgtcat caaacggcat atgccatgcg atggcggcag cctcctgcac
    24241 ggtcacatcg ctgacacggg aaaccaactc cagcggcgcg tccgcagaca ctttccctgg
    24301 cgctatcacg ctatacaacc accccacctt acctgtgttt tgcatcaact gcgctatatc
    24361 gctgatatca aaatggtaat tgagtttata gcaaggcgag cgcggctggc tgacctgaat
    24421 taatgcctct ccccagcgga aaatatcgcc catatgaaca ttactttccg tgagaccgtc
    24481 ggttgagaga ttttcgccaa acgcaggcgc aacaaacaac tccgcctgtt ccggaaattc
    24541 ccgcacccag tagagatagt gctcacgagg ataatgacac agcgctctgt ctggcccgcc
    24601 gtgaaccttt ttctccgcct gctcgtcacc ttccagcccc agctccgtca gcatcaactc
    24661 accatcaacc tggattttgg caattgcgct gggtttgcct tcgggataag cctgaatctt
    24721 gcctgtgtat acatcaaccg gatatcgcat ctgttccctc tccacgagaa atttccccat
    24781 tagaccatat ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc aggcaggaac
    24841 tcaatagcaa accggacgaa cgttagttca tccggctgtg gggattaagg aagcaggttt
    24901 atttcaccgt acctaaaatt tcgattttcg ctgctctggc tttgacttta cgtgaccaga
    24961 tagaggtaag aattggcacc agaatcgaag tcacaatgac cgccgtcgct accagcgaag
    25021 ttgctgccgg agccatcggt ttaaacgcag gcaccatttc tgcaatcagc acaggtgtcg
    25081 ctaccgcggc ccctgcggaa ctggaagcgg caattccggc agtgccatcg ccaccgccaa
    25141 tcaatttatc ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca
    25201 ggatccccag caaaccagtc tgggcaatca cagtcaaatc aatggtattg cccagcgcga
    25261 aggcaaagaa tggaatcagc gtttgcaccg ctttgctgaa aaattctcgt aattcagggt
    25321 caaggttccc cagggcaaag cccaccagga acggtaatac tgcgccgacg aaaacatgcg
    25381 gttcaaacga ggcaatccct gcagtgccca gaataatcat cgtcatgagc ggcccggact
    25441 ccaacgacat caacacaaat gccccagctt cttcttttgt gccgtactgc tgcatgatgg
    25501 aagcgtaaag tccgccattg gtcatatcca tcgccgccac cagcgccagc gttgaaagtc
    25561 cggcaaagaa tccaacttca acaccatgtt ccggaataat gcgcgaggca attgccgcaa
    25621 ccacccacgc gacggcaatt tttgttacca ccagcgtacc ggatttacgc aataccgttc
    25681 ccgtcgcgct taattttatt gacgccccca tgcaaaaaaa ccacaccgcc agaatgggca
    25741 ccgtaccggt aatcatcccg ttggtgaatg atccaaaata tttccccgcc cccggcgaga
    25801 aggtgtggca cagtgcgcca aggaatagcg ggacgagcat catccccccc gggattttct
    25861 caatcgagcg ttttatctgc atttccatca cctgtcggca tatttttaag ggctgatgcg
    25921 tcattcctca atggagcatc agtgttgatg gaaacgaaga tagacattca tttaatcaat
    25981 aaaagtgatc aaaaccacat caattgaaac gctgttttat ttttatcgga tcattgtttt
    26041 tattttgaaa ttgatcacaa aaaaaccacc gccgttggcg atggttcatt ttttaagctg
    26101 atatgcggcc tatacgcctc attgcagcag gcggcaaatg attatttttt cgccgcaaaa
    26161 cgtgccgctg cttcgtccca gttcaccacg ttccagaact ctttaatgta gtccggacgg
    26221 cggttctgga atttcaggta gtaagcatgt tcccacacat ccaggcccag aatcgggaag
    26281 ccggaagcgc cagaaatagc ttcacccatc agcggagaat cctggttagc agtagaaacc
    26341 accgccagtt tatcgccttt cagcaccagc catgcccagc cggaaccaaa gcgggaagct
    26401 gccgcttttt caaattctgc tttgaagtta tcaacggagc cgaagtcacg ttcgatagcc
    26461 gctttcaggt caccctgcag ggtggtgcct tttttcagac ctttccagaa caggctgtgg
    26521 ttagcgtgac cgccagcgtt gttgcgcagt acggttttct tgtctgctgg cagctggtcc
    26581 agtttggtga tcagctcttc aaccggcagg ttggcaaatt ctggcaggct ttccagcgcc
    26641 gcgttggcgt tgtttacgta ggtctgatgg tgtttggtgt ggtggatttc catggtctgc
    26701 ttatcgaagt gcggttccag ggcatcgtaa gcatacggca gggatggcag ggtatagctc
    26761 atattcatct ccagtattgt cgggcggccg attgttaatg ccgcgtaagc agttggttca
    26821 ttatagttaa ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc gtaagggtat
    26881 ggttttgcag gaaaatgccc gagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc
    26941 agtaaatcga caacggcggc aacaggcgaa aggttaatcg acagcacgat ttttacactc
    27001 atctcgtcgg agatgtgaag cgacgaaaaa tgatgaggat aagaagatga gtaacgcgat
    27061 tacgatgggg atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgctcc
    27121 gttcaaaaaa gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc
    27181 gtggattatt ctgccgtggg ccatcagcgc cctgttacta ccggatttct gggcgtatta
    27241 cagctcgttt agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat
    27301 cggtaatatc aactacggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat
    27361 cgccattggc attacgttga ttgtcggtac actgatgacg ccaattatca acggcaattt
    27421 cgatgtgctg attaacaccg aaggcggacg catgacgttg ctcggcgttc tggtggcgct
    27481 gattggcgta gggattgtaa ctcgagccgg gcagttgaaa gagcgcaaga tgggcattaa
    27541 agccgaagag ttcaatctga aaaaagggct ggtgctggcg gttatgtgcg gcattttctc
    27601 tgccgggatg tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcact
    27661 tggcgtcgat ccactgtatg tcgctctgcc aagctatgtt gtcatcatgg gcggcggcgc
    27721 gatcattaac ctcggtttct gttttattcg tctggcaaaa gtgaaggatt tgtcgctaaa
    27781 agccgacttc tcgctggcaa aaccgctgat cattcacaat gtgttactct cggcactggg
    27841 cgggttgatg tggtatctgc aattcttttt ctatgcctgg ggccacgccc gcattccggc
    27901 gcagtatgac tacatcagtt ggatgctgca tatgagtttc tatgtattgt gcggcggtat
    27961 cgtcggactg gtgctgaaag agtggagcaa tgcaggacgc cgtccggtaa cggtgttgag
    28021 cctcggttgt gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta
    28081 atctttctgc gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa
    28141 caccaccgaa aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa
    28201 caggcggcta tgctggagaa gatattgcgc atgacacact ctgacctgtc gcagatattg
    28261 attgatggtc attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga
    28321 tgcctcatca caaaatttat ccagcgcaaa gggacttttc aggctagccg ccagccgggt
    28381 aatcagctta tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg
    28441 atggcgattc agcaacatcg ccaactgccc gaacagcaac tctgccattt cgttagcaaa
    28501 cggcacatgc tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat
    28561 ccccacgcta cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc
    28621 cccctgccag tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac
    28681 cagatcgtta acggaagcgt aggagtgttt atcgtcagca tgaatgtaaa agagatcgcc
    28741 acgggtaatg cgataagggc gatcgttgag tacatgcagg ccattaccgc gccagacaat
    28801 caccagctca caaaaatcat gtgtatgttc agcaaagaca tgttgcggat aacggtcagc
    28861 cacagcgact gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg
    28921 cgccaccgtg gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa
    28981 tacgttgaga agattcgcgt tattgcagaa aaccatcccg tccctgacga atatcacgcg
    29041 gtgaccagtt aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccgc
    29101 aatgataggc gatgtcagta acgctggcct cactgtggcg tagcagatgt cgggccttca
    29161 tcagtcgcag gcggttcagg taacgctgag gcgtcagtcc cgtttgctgc ttaagctgcc
    29221 gatgtagcgt tcgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat
    29281 cggcaaaatg gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca
    29341 ggttcttctg caaactgctt ttacgcagca agagcagtaa ttgcataaac aaagatctcg
    29401 cgactggcgg tcgagggtaa atcattttcc ccttcctgct gttccatctg tgcaaccagc
    29461 tgtcgcacct gctgcaatac gctgtggtta acgcgccagt gagacggata ctgcccatcc
    29521 agctcttgtg gcagcaactg attcagcccg gcgagaaact gaaatcgatc cggcgagcga
    29581 tacagcacat tggtcagaca cagattatcg gtatgttcat acagatgccg atcatgatcg
    29641 cgtacgaaac agaccgtgcc accggtgatg gtatagggct gcccattaaa cacatgaata
    29701 cccgtgccat gttcgacaat cacaatttca tgaaaatcat gatgatgttc aggaaaatcc
    29761 gcctgaggga gccggggttc tatcgccacg gacgcgttac cagacggaaa aaaatccaca
    29821 ctatgtaata cggtcatact ggcctcctga tgtcgtcaac acggcgaaat agtaatcacg
    29881 aggtcaggtt cttaccttaa attttcgacg gaaaaccacg taaaaaacgt cgatttttca
    29941 agatacagcg tgaattttca ggaaatgcgg tgagcatcac atcaccacaa tgaagcaaat
    30001 tgtgaacatc atcacgttca tctttccctg gttgccaatg gcccattttc ctgtcagtaa
    30061 cgagaaggtc acgaattcag gcgcttttta gactggtcgt aatgaaattc agcaggatca
    30121 cattatgacc tttcgcaatt gtgtcgccgt caatctcggc gcatccagtg ggcgcgtgat
    30181 gctggcgcgt tacgagcgtg aatgccgcag cctgacgctg cgcgaaatcc atcgttttaa
    30241 aaatgggctg catagccaga acggttatgt cacctgggat gtggatagcc tggaaagtgc
    30301 cattcgcctt ggcttaaaca aagtgtgtga ggaagggatt cgtatcgata gcattgggat
    30361 tgatacctgg ggcgtggact ttgtgctgct cgaccaacag ggtcagcgtg tgggcctgcc
    30421 cgttgcttat cgcgatagcc gcaccaatgg cctaatggcg caggcacagc aacaactcgg
    30481 caaacgcgat atttatcaac gtagcggcat ccagtttctg cccttcaata cgctttatca
    30541 gttgcgtgcg ctgacggagc aacaacctga acttattcca cacattgctc acgctctgct
    30601 gatgccggat tacttcagtt atcgcctgac cggcaagatg aactgggagt acaccaatgc
    30661 caccaccaca caactggtca atatcaatag cgacgactgg gacgagtcgc tactggcgtg
    30721 gagcggggcc aacaaagcct ggtttggtcg cccgacgcat ccgggtaatg tcattgggca
    30781 ctggatttgc ccgcagggta atgagattcc ggtggtcgcc gttgccagcc atgataccgc
    30841 cagcgcggtt atcgcctcgc cgttaaacgg ttcacgcgcc gcttatctct cttctggcac
    30901 ctggtcattg atgggcttcg aaagccagac gccatttacc aatgacacgg cgctggcagc
    30961 caacatcacc aatgaaggcg gggcggaagg tcgctatcgg gtgctgaaaa atattatggg
    31021 cttatggctg cttcagcgag tgcttcagga acggcaaatc aacgatctcc cggcgcttat
    31081 cgccgcgaca caggcacttc cggcctgccg cttcatcatc aatcccaatg acgatcgctt
    31141 tattaatcct gaggcgatgt gcagcgaaat tcaggctgcg tgtcgggaaa cggcgcaacc
    31201 gatcccggaa agtgatgctg aactggcgcg ctgtattttc gacagtctgg cgctgctgta
    31261 tgccgatgtg ttgcatgagc tggcgcagct gcgcggtgaa gatttctcgc aactgcatat
    31321 tgtcggcggc ggctgccaga acacgctgct caaccagcta tgcgccgatg cctgcggtat
    31381 tcgggtgatc gccgggcctg ttgaagcctc aacgctcggc aatatcggca tccagttaat
    31441 gacgctggat gaactcaaca atgtggataa tttccgtcag gtcgtcagca ccaccgcgaa
    31501 tctgaccacc tttaccccta atcctgacag tgaaattgcc cactatgtgg cgcagattca
    31561 ctctacacga cagacaaagg agctttgcgc atgaccactc aactggaaca ggcctgggag
    31621 ctggcgaaac agcgtttcgc ggcggtgggg attgatgtcg aggaggcgct gcgccaactt
    31681 gatcgtttac ccgtttcaat gcactgctgg cagggcgatg atgtttccgg ttttgaaaac
    31741 ccggaaggtt cgctgaccgg tgggattcaa gctactggta attatccggg caaagcgcgt
    31801 aatgccagtg agctacgtac cgatctggaa caggctatgc ggctgattcc ggggccgaaa
    31861 cggcttaatt tacatgccat ctatctggaa tcagacacgc cagtctcgcg cgaccagatc
    31921 aaacctgagc acttcaaaaa ctgggttgaa tgggcgaaag ccaatcagct cggtctggat
    31981 tttaacccct cctgtttttc gcatccgcta agcgccgatg gctttacgct ttcccatgcc
    32041 gacgacagca ttcgccagtt ctggattgat cactgcaaag ccagccgtcg cgtttcggcc
    32101 tattttggcg agcaactcgg cacaccatcg gtgatgaaca tctggatccc ggatggtatg
    32161 aaagatatca ccgttgaccg tctcgccccg cgtcagcgtc tgctggcagc actggatgag
    32221 gtgatcagcg agaagctaaa ccctgcgcac catatcgacg ccgttgagag caaattgttt
    32281 ggcattggcg cggagagcta cacggttggc tccaatgagt tttacctggg gtatgccacc
    32341 agccgccaga cggcgctgtg cctggacgcc gggcacttcc acccgactga agtgatttcc
    32401 gacaagattt ccgccgccat gctgtatgtg ccgcagttgc tgctgcacgt cagtcgtccg
    32461 gttcgctggg acagcgatca cgtagtgctg ctggatgatg aaacccaggc aattgccagt
    32521 gagattgtgc gtcacgatct gtttgaccgg gtgcatatcg gccttgactt cttcgatgcc
    32581 tctatcaacc gcattgccgc gtgggtcatt ggtacacgca atatgaaaaa agccctgctg
    32641 cgtgcgttgc tggaacctac cgccgagctg cgcaagttgg aagcggcggg cgattacact
    32701 gcgcgtcagg cactgctgga agagcagaaa tcgttgccgt ggcaggcggt atgggaaatg
    32761 tattgtcagc gtcacgatac cccagcgggt agcgaatggc tggagagcgt gcgggcttat
    32821 gagaaagaaa ttttgagtca gcgtgggtaa acactgccag atgcggcacg agcgccttat
    32881 ccggcctaca gatcgtgcat ctgacaacac cccatttaca ggaacaccga acatgcaaaa
    32941 cattactcag tcctggtttg tccagggaat gatcaaagcc accaccgacg cctggctgaa
    33001 aggctgggac gagcgcaacg gcggcaacct gacgctacgc ctggatgacg ctgacatcgc
    33061 accgtatcac gacaatttcc accaacaacc gcgttatatc ccgctcagcc aacccatgcc
    33121 tttactggca aatacaccgt ttatcgtcac cggctcgggc aaattcttcc gtaacgtcca
    33181 gcttgatcct gcggctaact taggcgtcgt aaaagtcgac agcgacggcg cgggctacca
    33241 tattctctgg gggttaacca acgaggccgt tccaacttct gaacttccgg cacactttct
    33301 ctcccattgc gaacgcatta aagccaccaa cggtaaagat cgggtgatca tgcactgcca
    33361 tgccaccaac ctgatcgccc tcacctatgt actggaaaac gacacggcgg tcttcactcg
    33421 ccaactgtgg gaaggcagca ccgagtgcct ggtggtattc ccggatggcg ttggcattct
    33481 gccgtggatg gtgcccggca cggacgaaat cggtcaggcg accgcacaag aaatgcaaaa
    33541 acattcgctg gtgttgtggc ccttccacgg tgtgttcggt tccggaccga cactggatga
    33601 agcctttggt ttgatagata ccgcagagaa atcagcggag gtgttagtca aaatctactc
    33661 gatgggcggt atgaagcaga ccattacccg tgaggagctg attgcactcg gtgagcgttt
    33721 tggcgttacg ccactggcca gtgcgctggc actttaatat cccaacagat atccggcggt
    33781 gtgggaaacg tcgccggaca gatggatttg aacttaagga gagtcattat gagttttatg
    33841 ttggcattac cgaaaattag cctgcatggt gctggggcaa ttggcgatat ggtaaatctg
    33901 gttgcgcata aacagtgggg aaaagcactg atcgttaccg atgggcaact ggttaagttg
    33961 ggattactgg acagcctgtt tatcgcactg gatcaatacg aaatgtctta tcacttattt
    34021 gatgaggtat ttcctaaccc cactgaagaa ctggtgcaaa aaggctacgc tgcatttcag
    34081 gatgccgcat gtgactacat tattgctttc ggtggcggta gcccaattga tactgcaaaa
    34141 gccgtaaaaa tccttaccgc taatcctgcg ccttctacgg cgtactctgg tgttggtaaa
    34201 gtgacaaatc ccggcgtacc gctggtggca atcaatacga ctgccggaac agctgctgag
    34261 atgaccagca atgcggtgat catcgacacc gcgcggcagg taaaagaggt cattatcgac
    34321 ccgaatatta ttcccgatat tgcggtggat gatgccagtg taatgctgga gatcccggcc
    34381 tcggttaccg ccgccacggg tatggatgca ctgactcacg cagttgaagc ttatgtttct
    34441 gttggtgcac atccattaac cgacgctaac gcactggaag ctatccgttt aattacgctg
    34501 tggctgccaa aagcggtaga tgatggtcat aatctggaag ctcgtgaaca aatggcattc
    34561 gcccagtacc tggcgggtat ggcatttaac agtgctggtc tgggccttgt tcatgcactt
    34621 gcgcatcagc ccggcgccac ccacaacctg ccgcatggtg tgtgcaatgc tatcttgttg
    34681 ccagttattg aaaactttaa ccgcccgaat gccatcgcac gctttgcccg cattgctcag
    34741 gcaatgggtg tcgatacgcg tgatatgagt gaagaagcgg caagtactga agcaattaac
    34801 gctatccgcg cattaagcca tcgcgtgggt attccggcag gattcagcca gcttggcatc
    34861 accaaagaag atattgaagg ctggttagat aaagccctgg ctgacccgtg tgtgccatgt
    34921 aacccgcgta ccgccagccg cgacgaggtg cgcgagctgt atctggaggc attatgatcc
    34981 gtaaggcatt tgtgatgcag gttaaccccg acgctcacga agagtatcaa cgtcggcata
    35041 accccatctg gccggaactg gaagcagtgt tgaaatctca cggtgcgcat aactacgcca
    35101 tttacctcga caaagcgcgt aatctgctgt ttgccacggt agagattgaa tctgaagagc
    35161 gctggaatgc ggttgccagt actgatgttt gccaacgttg gtggaaatat atgaccgacg
    35221 tcatgcccgc gaacccggat aacagcccgg tgagtagcga actgcaagaa gtgttttacc
    35281 tgccgtaatt gcctaatgtg ccggagacgc ctccggtaca atatttttaa tttcgatttg
    35341 aaaaataaac atattacacc ttcagttaat atatctcggt aacttatatt tccccgatgc
    35401 attgtgagta aatcacaaaa ataatgaata actcattaat gattcatgtg gtttatttaa
    35461 ataaccatta tgtgcattac tccgcaaatc tggcctttca ctctgtctca aattgtcatt
    35521 gtttaccgac atatcacctc tcatcactta atcgctccac cattacaatt gattaaaata
    35581 tattttatgg agtccgatta atggcagctc ttactgcaag ctgtattgac ctgaatattc
    35641 agggcaatgg cgcttattcc attctgaagc agttggcgac aatggcgtta caaaacggtt
    35701 ttatcaccga ctcacaccag ttcctgcaaa ccctgctgct gcgcgaaaaa atgcactcta
    35761 ctggatttgg ttccggcatc gccgtgccgc acggtaaaag cgcctgcgtt aaacaaccgt
    35821 tcgtgttatt cgcccgcaaa gcgcaggcca ttgactggaa agccagcgac ggcgaagacg
    35881 tcaattgctg gatctgcctc ggcgtgccgc aaagtggcga agaggatcag gtcaaaatca
    35941 tcggcacact gtgtcgcaaa attatccatc aggattttat caaccaactg cgacagggcg
    36001 acgccgacca ggtacttgct ttgttaaatc aaaccctcag ctcataagga agtggcgatg
    36061 gagtcatcct tacgtattgt cgcgatcacc aactgccccg ccgggatcgc tcacacctac
    36121 atggtggcgg aagccctgga acagaaagcc cgttctctcg gccataccat aaaagtggaa
    36181 actcaagggt ccagcggcgt tgaaaaccgc ttatccagcg aagagattgc cgctgccgat
    36241 tacgtcattc tagctaccgg gcgtggcctg agcggtgatg accgggcgcg gtttgccggg
    36301 aagaaagttt atgagattgc catctcccag gcgctgaaaa atatcgacca gattttcagc
    36361 gaattaccaa caaactcgca gctttttgcc gcagatagcg gcgtgaagct cggcaaacag
    36421 gaagtgcaaa gcggcagcgt gatgagccac ctgatggcgg gcgtctctgc ggcgctgcca
    36481 tttgtcatcg gcggcggtat tctggtggcg cttgccaaca tgctggtgca gttcggtttg
    36541 ccatataccg atatgtcgaa aggtgcgccg tcgtttacct gggtagttga atccatcggt
    36601 tacctcggct tcacctttat gatccccatc atgggcgctt atattgcctc gtcgattgcc
    36661 gataaacccg ctttcgctcc ggcatttctg gtttgctatc tggcgaacga taaagccctg
    36721 ctcggcaccc agtcaggcgc gggcttcctc ggcgcggtgg tgctggggct ggcgattggc
    36781 tatttcgtct tctggttccg taaagtccgt cttggcaaag cattacaacc gctgctcggc
    36841 tccatgctta tcccattcgt tactctgctg gtttttggcg tgctcactta ctacgttatc
    36901 gggccggtga tgtccgacct catgggcggg ctgctccact tcctgaatac cattcctcca
    36961 tcaatgaagt ttgcggcagc gtttctggtc ggtgcgatgc tggcgttcga tatgggcggc
    37021 cccatcaata aaaccgcctg gttcttctgc ttctcactgc tggaaaaaca catttatgac
    37081 tggtacgcca tcgtcggcgt tgtcgcgctg atgccgcccg tcgcggcggg tcttgcgacg
    37141 ttcattgcgc ccaaactgtt tacccgacag gaaaaagagg ctgccagtag tgccattgtg
    37201 gtcggtgcca cagtcgcaac cgaaccggct attccttacg ctctggcagc tccgttgccg
    37261 atgattaccg ccaatacgct ggcgggcggt attaccggcg tgctggtgat cgctttcgga
    37321 atcaaacgtc tggcacctgg tttgggtatc tttgacccgc tgatcggcct gatgtcaccg
    37381 gtaggttcgt tttatctggt gctggcgatt ggcctcgcgc tgaacatttc attcattatc
    37441 gtcctgaaag gattgtggtt acgccgcaaa gcgaaagccg cgcaacagga gcttgtccat
    37501 gaacattgag ttactgcaac agttgtgcga agccagcgcc gttagcggcg atgaacagga
    37561 agttcgcgac attctgataa acacgctgga accttgcgtg aatgaaatca cctttgatgg
    37621 gctgggcagc tttgtcgccc gtaagggaaa taaagggccg aaagttgccg ttgtcgggca
    37681 tatggatgaa gtcggcttta tggtcaccca catcgacgag agcggttttc tgcgtttcac
    37741 caccattggt ggctggtgga atcagtcgat gctcaaccac cgggtaacca tacgcacaca
    37801 caagggagtg aaaatccctg gtgtgattgg ttccgtcgcg cctcatgcgt taacggaaaa
    37861 gcaaaagcaa cagccgcagt catttgatga gatgttcatt gatattggcg caaacagtcg
    37921 cgatgaggtg gaaaaacgcg gtgtggcgat gggcgatttt attagcccgg aagccaattt
    37981 tgcctgctgg ggcgaagata aagtggtcgg caaggcgttg gataaccgca tcggctgcgc
    38041 aatgatggct gagctactac agacggtgaa taatcccgaa attacgctgt atggcgttgg
    38101 cagtgtggaa gaagaagttg ggctacgcgg ggcgcaaacc tcggctaaac acattaaacc
    38161 ggatgtggtg atcgtgctgg acaccgctgt cgcgggcgat gttccaggca ttgataacat
    38221 taaatatccg ctgaaactgg gcaacgggcc ggggttgatg ctgtttgaca agcgctactt
    38281 ccccaaccag aaactggtag cagcgttaaa aagctgtgcc acgcagaatg atttaccgct
    38341 gcaattttcc accatgaaaa ccggtgcgac ggatggcggg cgctacaacg tgatgggcgg
    38401 tgggcgtccg gttgtcgcgc tgtgtttgcc aacgcgttat ctgcacgcca acagcggcat
    38461 gatttcaaaa gccgattacg atgctctgct cacgctgata cggggttttc tgaccacctt
    38521 aaccgcggaa aaagtcagcg cgtttagcca gttccgtcag gtggattaat gctaaacgaa
    38581 cgccagttaa agattgtcga tctgctggag caacagccgc gcacgcctgg cgagctggcg
    38641 caacagactg gcgtttctgg caggaccata ctgcgtgata ttgactatct caactttacc
    38701 cttaacggca aagcccgcat ttctgccagt ggtagtgcgg gctatcagct ggaaatcttc
    38761 gagcgccgca gcttttttca gttgctgcaa aagcacgata acgacgatcg gctgctggca
    38821 ctgttattac tgaatacctt tactccccgt gcgcaactcg cctcggcgct taatttgccg
    38881 gaaacgtggg tcgcagagcg tctgccccgg ttaaaacagc gttatgaacg cacttgctgc
    38941 ctggccagtc gccccggttt gggccatttc attgatgaga cagaagaaaa acgcgttata
    39001 ttgctggcga acttgctgcg caaagatccg tttttaattc cgctggcggg cgtaacacga
    39061 gacaaccttc agcatttatc cacggcctgc gacaaccaac accgctggcc gctcatgcag
    39121 ggtgattatc tctccagcct gattctggcg atttacgccc tgcgtaatca actgaccgat
    39181 gagtggccgc aatatcccgg tgacgagata aaacaaatcg ttgaacatag cggtctgttt
    39241 cttggtgata acgctgtaag aaccctgaca ggtttgatag agaaacagca tcagcaagcg
    39301 cagataattt ctgccgataa tgtgcagcgg ttgctgcaaa gggtgccggg catcgcgtca
    39361 ttgaatatta ttgatacgca gctggttgag aacattaccg ggcatttatt acgttgcctt
    39421 gccgcaccgg tgtggattgc tgagcaccgc caaagcagca tgaataacct gaaagccgcc
    39481 tggcctgcgg cctttgatat gagtctgcac tttattacgc tactgcgtga acagctcgat
    39541 attccccttt tcgacagcga tctgatcggt ttgtattttg cctgtgcgct ggaacggcat
    39601 caaaacgaac gccagccgat cattttgctc tcggaccaga acgcgattgc cactattaat
    39661 cagctcgcca ttgagcgcga tgttttacat tgtcgggtga ttattgcccg tagcttaagc
    39721 gaacttgttg ccattcgcga agagattgag ccgttattga tcattaacaa cagccattat
    39781 ttactggatg acgcggtaaa taattacatc accgtaaaaa atatcattac ggctgccggt
    39841 atcgaacaaa taaaacattt tctggcgacg gcatttattc gccaacagcc ggagcgtttt
    39901 ttctctgctc ccggaagttt tcattattcg aatgtacgcg gtgaaagctg gcaacatatt
    39961 acccggcaaa tttgtgcgca attagtcgca caacaccata ttaccgccga tgaagcacaa
    40021 cgcatcatcg cccgcgaagg cgaaggtgaa aacctgattg ttaatcgcct cgccatccca
    40081 cattgctgga gcgaacagga gcgacgtttt cgtggatttt ttattaccct cgcccaacca
    40141 gttgaggtga ataacgaagt cattaaccat gtcttgatcg cctgcgccgc cgccgatgcg
    40201 cgtcatgagc taaaaatatt tagctatctg gcaagcatat tgtgtcagca tccggcggag
    40261 attattgccg ggttaacagg atatgaggca tttatggagt tacttcacaa ggggtgaact
    40321 gtgtcggatg cgccgcatcc gacactgcat ttatcatcaa aaacgtttaa cgcaggctat
    40381 tatagtcatc aaccatttcg ttgtactcgc gtaatgctct cgggaaagag tcttcgacca
    40441 tccagcttga gtttgcgtcc tgaagttgct ctttatccca gtcagagtac gggactttat
    40501 ctctgatgcg gcgaacgtat tttttagcct cgagttgata ctggcctgcc gaattaataa
    40561 acgagaaatt catgccgcct ttatccgctt ctttggcctg cgccactagc gtttccagtt
    40621 cagacacttt cttcattgcc gcatcaacat caaacttctc ttgcgatatc aggttattaa
    40681 tttgtttcgc tgaaatcatg actgccagag agtagtagtg gaatgttttc ccttctcttt
    40741 cttcaatatt tttcagttcc gcaagctggc gcttatcgtt aatttcctga atcgccgcat
    40801 gataagattc tgctacaggt tcaaaggctt ccagattttt taagaatgtc tggtgcagcg
    40861 ttttcccttt cgcaaacgca tcgtctttat aattttcctg ggtgtaatat ttatccattt
    40921 cgttaatggt attacccaac gccacggcag catcgatata actcaccgcc acgccatcaa
    40981 ttggttgcag cgcaggcgtt aacgccacag cactttttac gcctttttca cactcagcga
    41041 ggttggattc actaatgccg tagatgccat aaacagtacg ctcttcaccg gtaggcccct
    41101 gtttaaaatc tttcagccag tcagcataac gcgccagact gctctgtaca gggatctgta
    41161 acttgttata gcacttgata tagacatcca ttttttcgtc gaaaaccgct tgttcatcga
    41221 ccaccggttg cgcaggtgtt tcaggtttag cttctgcttt tgcaacggga gcttcaggag
    41281 ctggtgcggc aggttgacta ttggcaggcg ggggcgtttc tgtttcggct tttttatcat
    41341 cacaacccgt cagaccgagg gccatgatgg cgactataat agcggaagat aataaat
//
LOCUS       DAZGOB010000033        41301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGOB010000033 DAZGOB010000000
VERSION     DAZGOB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.033"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..193
                     /locus_tag="ACHLIF_001788"
     CDS             <1..193
                     /locus_tag="ACHLIF_001788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605820.1"
                     /translation="DSGIPRFESSYPSHIKKARFGELFAFLRSFNVECDVDTSYPSMS
                     DATLPRLIRPTVGTHPAML"
     gene            complement(196..1371)
                     /gene="ubiF"
                     /locus_tag="ACHLIF_001789"
     CDS             complement(196..1371)
                     /gene="ubiF"
                     /locus_tag="ACHLIF_001789"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO9605821.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGGNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1517..2941
                     /gene="miaB"
                     /locus_tag="ACHLIF_001790"
     CDS             1517..2941
                     /gene="miaB"
                     /locus_tag="ACHLIF_001790"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO9605822.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3094..4134
                     /gene="ybeZ"
                     /locus_tag="ACHLIF_001791"
     CDS             3094..4134
                     /gene="ybeZ"
                     /locus_tag="ACHLIF_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO9605823.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4131..4598
                     /gene="ybeY"
                     /locus_tag="ACHLIF_001792"
     CDS             4131..4598
                     /gene="ybeY"
                     /locus_tag="ACHLIF_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO9605824.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4688..5566
                     /gene="corC"
                     /locus_tag="ACHLIF_001793"
     CDS             4688..5566
                     /gene="corC"
                     /locus_tag="ACHLIF_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO9605825.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5591..7129
                     /gene="lnt"
                     /locus_tag="ACHLIF_001794"
     CDS             5591..7129
                     /gene="lnt"
                     /locus_tag="ACHLIF_001794"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO9605826.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGTGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7207..9084)
                     /locus_tag="ACHLIF_001795"
     CDS             complement(7207..9084)
                     /locus_tag="ACHLIF_001795"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO9605827.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            9539..10447
                     /gene="gltI"
                     /locus_tag="ACHLIF_001796"
     CDS             9539..10447
                     /gene="gltI"
                     /locus_tag="ACHLIF_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO9605828.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10617..11357
                     /gene="gltJ"
                     /locus_tag="ACHLIF_001797"
     CDS             10617..11357
                     /gene="gltJ"
                     /locus_tag="ACHLIF_001797"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO9605829.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            11357..12031
                     /gene="gltK"
                     /locus_tag="ACHLIF_001798"
     CDS             11357..12031
                     /gene="gltK"
                     /locus_tag="ACHLIF_001798"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO9605830.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12031..12756
                     /gene="gltL"
                     /locus_tag="ACHLIF_001799"
     CDS             12031..12756
                     /gene="gltL"
                     /locus_tag="ACHLIF_001799"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO9605831.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            12874..13809
                     /gene="rihA"
                     /locus_tag="ACHLIF_001800"
     CDS             12874..13809
                     /gene="rihA"
                     /locus_tag="ACHLIF_001800"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO9605832.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            13893..15563
                     /gene="hscC"
                     /locus_tag="ACHLIF_001801"
     CDS             13893..15563
                     /gene="hscC"
                     /locus_tag="ACHLIF_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO9605833.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQNSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(15623..17074)
                     /gene="djlC"
                     /locus_tag="ACHLIF_001802"
     CDS             complement(15623..17074)
                     /gene="djlC"
                     /locus_tag="ACHLIF_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO9605834.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(17071..17778)
                     /gene="ybeU"
                     /locus_tag="ACHLIF_001803"
     CDS             complement(17071..17778)
                     /gene="ybeU"
                     /locus_tag="ACHLIF_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO9605835.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            17879..18433
                     /gene="ybeT"
                     /locus_tag="ACHLIF_001804"
     CDS             17879..18433
                     /gene="ybeT"
                     /locus_tag="ACHLIF_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO9605836.1"
                     /translation="MNKKLMYIFAIFIVAAITCISHPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(18443..19870)
                     /gene="djlB"
                     /locus_tag="ACHLIF_001805"
     CDS             complement(18443..19870)
                     /gene="djlB"
                     /locus_tag="ACHLIF_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO9605837.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(19867..20574)
                     /gene="ybeR"
                     /locus_tag="ACHLIF_001806"
     CDS             complement(19867..20574)
                     /gene="ybeR"
                     /locus_tag="ACHLIF_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO9605838.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            20738..21715
                     /gene="ybeQ"
                     /locus_tag="ACHLIF_001807"
     CDS             20738..21715
                     /gene="ybeQ"
                     /locus_tag="ACHLIF_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO9605839.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(21785..22267)
                     /gene="ybeL"
                     /locus_tag="ACHLIF_001808"
     CDS             complement(21785..22267)
                     /gene="ybeL"
                     /locus_tag="ACHLIF_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO9605840.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            22502..25084
                     /gene="leuS"
                     /locus_tag="ACHLIF_001809"
     CDS             22502..25084
                     /gene="leuS"
                     /locus_tag="ACHLIF_001809"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO9605841.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            25099..25680
                     /gene="lptE"
                     /locus_tag="ACHLIF_001810"
     CDS             25099..25680
                     /gene="lptE"
                     /locus_tag="ACHLIF_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO9605842.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            25680..26711
                     /gene="holA"
                     /locus_tag="ACHLIF_001811"
     CDS             25680..26711
                     /gene="holA"
                     /locus_tag="ACHLIF_001811"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO9605843.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            26713..27354
                     /gene="nadD"
                     /locus_tag="ACHLIF_001812"
     CDS             26713..27354
                     /gene="nadD"
                     /locus_tag="ACHLIF_001812"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO9605844.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            27378..27989
                     /gene="cobC"
                     /locus_tag="ACHLIF_001813"
     CDS             27378..27989
                     /gene="cobC"
                     /locus_tag="ACHLIF_001813"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO9605845.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            28249..28566
                     /gene="rsfS"
                     /locus_tag="ACHLIF_001814"
     CDS             28249..28566
                     /gene="rsfS"
                     /locus_tag="ACHLIF_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO9605846.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            28570..29037
                     /gene="rlmH"
                     /locus_tag="ACHLIF_001815"
     CDS             28570..29037
                     /gene="rlmH"
                     /locus_tag="ACHLIF_001815"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO9605847.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            29068..30969
                     /gene="mrdA"
                     /locus_tag="ACHLIF_001816"
     CDS             29068..30969
                     /gene="mrdA"
                     /locus_tag="ACHLIF_001816"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO9605848.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            30972..32084
                     /gene="mrdB"
                     /locus_tag="ACHLIF_001817"
                     /gene_synonym="rodA"
     CDS             30972..32084
                     /gene="mrdB"
                     /locus_tag="ACHLIF_001817"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO9605849.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            32095..33183
                     /gene="rlpA"
                     /locus_tag="ACHLIF_001818"
     CDS             32095..33183
                     /gene="rlpA"
                     /locus_tag="ACHLIF_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO9605850.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            33323..34534
                     /gene="dacA"
                     /locus_tag="ACHLIF_001819"
     CDS             33323..34534
                     /gene="dacA"
                     /locus_tag="ACHLIF_001819"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO9605851.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            34645..34908
                     /gene="ybeD"
                     /locus_tag="ACHLIF_001820"
     CDS             34645..34908
                     /gene="ybeD"
                     /locus_tag="ACHLIF_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO9605852.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            35009..35650
                     /gene="lipB"
                     /locus_tag="ACHLIF_001821"
     CDS             35009..35650
                     /gene="lipB"
                     /locus_tag="ACHLIF_001821"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO9605853.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            35909..36862
                     /gene="ybeF"
                     /locus_tag="ACHLIF_001822"
     CDS             35909..36862
                     /gene="ybeF"
                     /locus_tag="ACHLIF_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO9605854.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            37071..38036
                     /gene="lipA"
                     /locus_tag="ACHLIF_001823"
     CDS             37071..38036
                     /gene="lipA"
                     /locus_tag="ACHLIF_001823"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO9605855.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(38137..38340)
                     /gene="tatE"
                     /locus_tag="ACHLIF_001824"
     CDS             complement(38137..38340)
                     /gene="tatE"
                     /locus_tag="ACHLIF_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO9605856.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(38469..39257)
                     /locus_tag="ACHLIF_001825"
     CDS             complement(38469..39257)
                     /locus_tag="ACHLIF_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO9605857.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            39350..39733
                     /gene="crcB"
                     /locus_tag="ACHLIF_001826"
     CDS             39350..39733
                     /gene="crcB"
                     /locus_tag="ACHLIF_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO9605858.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(39788..39997)
                     /gene="cspE"
                     /locus_tag="ACHLIF_001827"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(39788..39997)
                     /gene="cspE"
                     /locus_tag="ACHLIF_001827"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO9605859.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(40172..40732)
                     /gene="pagP"
                     /locus_tag="ACHLIF_001828"
     CDS             complement(40172..40732)
                     /gene="pagP"
                     /locus_tag="ACHLIF_001828"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO9605860.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT        10443 a        10485 c        10221 g        10152 t
ORIGIN      
        1 tgattccggc attccgaggt tcgaatcctc gtaccccagc cacattaaaa aagctcgctt
       61 cggcgagctt tttgcttttc tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta
      121 tccttcaatg tcggatgcga cgctgccgcg tcttatccga cctacggttg gcacgcatcc
      181 ggcaatgttg taaggctaca accctaacgc atatttcagc gcctgacgtt tcaacacgcc
      241 agcacgctct gccgccatta acccgagatt acgcacaaaa cgcagcggcg gcagattatt
      301 actgaatccg gcataaaaca gatccatacc actttgcata atgaagttat ccgccatgcg
      361 ccgcatctgg taacgcttga ggacaggata actggcccac gcttcaccgt agctgcgggc
      421 attcaccaga acatcaatca gggcatcgac atcacgataa ccaagattcc ccccctgccc
      481 cgccagcgga tggatggtat gcgcggcatc gcccaccagc gcaagccccg gctgcacata
      541 ctgcaacgca tgtcggcgcg tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc
      601 cagacgcgac gggaaatgct tcgcgatttc cgcctggagt tgcgccatat tcatattctg
      661 caactggcga atacgtaccg gagagtcata ccacaccagc gatgcccagt tatcaaacaa
      721 cggcagaaac gcacgcggtc cgtccggggt aaattgctgc caggtgctgt cgccgggatc
      781 gttctcacac tgtacgctaa tcaacataca cgactgcgca tactgccagg catgaacgcc
      841 aattcccgcc atctgccgca cctgcgaatt tgcgccgtca gcaccaatca ccagcttcgc
      901 gcgaatcact tcaccgccct tcagttccag ctcctgaaga tcattatctc gatgtaatgc
      961 aatcagcgag cctggcacac gtaacgttac tttcggatgc gcttccagcg cctgccacaa
     1021 tgcctgttgc aggacggtgt tttccaccat atagccaagc agcggcagct taagttcagc
     1081 ggcgtcaaac atcacatgcg ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg
     1141 gcaacgcata gcctgtactg catcccagac ccctaaccct ttaagcaatg ataccgatgc
     1201 ggcgctgatc gccgagatcc gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc
     1261 tgcgtgctcg atcaccgtta ccgaaaatcc gtgctgtgcc agccccagcg ccagtgcgcc
     1321 gccgaccatt cctccgccga caatggcaat ttccgttggt tgatttgtca tggtcaatca
     1381 tcctgttagc aatatgcttt aagtttacag gatttttacc cccaagggct gataacgctc
     1441 atactggtca caacggcagc aaagcattac actatgcgcc ctgcattcct ggctactatt
     1501 tcgcaagagc aagtcgatga ccaaaaaact ccatattaaa acctggggct gtcagatgaa
     1561 cgagtacgat tcatcgaaga tggccgatct gctggatgcc acccacggct atcaactgac
     1621 cgacgtggcg gaagaagcgg atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc
     1681 tcaggaaaaa gtcttccatc agttgggtcg ctggaaactg ttaaaagaga agaatccaga
     1741 cctgattatc ggcgtcggtg gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg
     1801 cgcccactat gtcgatatta tttttgggcc gcaaacgctg caccgtctgc cggagatgat
     1861 caactccgtg cgcggcgacc gcagcccggt tgtagatatc agcttcccgg aaatcgagaa
     1921 gtttgaccgt ctgccggaac cgcgcgccga agggccgacc gcgtttgtct ccatcatgga
     1981 aggctgcaat aaatattgca cctactgcgt ggtgccttac acccgtggtg aagaggtaag
     2041 ccgtccgtcc gacgatattc tgtttgagat cgcccagctt gcggctcagg gcgtgcgtga
     2101 agtcaacctg ctcggtcaga atgtgaatgc ctggcgtggt gagaactacg acggcaccac
     2161 cggatcgttt gccgatctgt tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg
     2221 ctttaccacc agccatccga tcgaattcac cgacgatatc atcgaagtgt atcgcgacac
     2281 gccggagctg gtgagcttcc tgcatctgcc ggtacagagc ggttccgatc gcattctgaa
     2341 cctgatgggc cgtactcata cggcactgga gtacaaagcg atcatccgta aactgcgtgc
     2401 ggcgcgtccg gatattcaga tcagttctga cttcattgtt ggcttccctg gcgaaaccac
     2461 cgaagacttc gagaaaacga tgaagctgat tgccgacgtc aatttcgaca tgagctacag
     2521 ctttatcttc tctgcacgtc cgggtacacc agccgccgat atggttgatg atgttccgga
     2581 agaagagaag aagcaacgtc tgtatattct gcaagaacgt atcaatcagc aagcgatggc
     2641 atggagccgc cggatgctcg gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa
     2701 gagcatcatg gagctttccg ggcgtaccga aaataaccgc gtggtcaact tcgagggcac
     2761 accggatatg atcggtaaat tcgtcgatgt agaaattacc gacgtctacc cgaactctct
     2821 gcgcggtaaa gtggtgcgta ctgaagatga aatgggtctg cgcgtggcag aaacaccgga
     2881 atcggtgatt gcccgtaccc gcaaagaaaa cgaccttggc gtgggttatt atcagccgta
     2941 attctcaggc ccgccgttcc ggtgggcctt gttttttctc tctttatccc catctttatc
     3001 gcaatgcttg cgcctttttc ctgtggcgtg aataatttcc ttataggccg gtgaatgttc
     3061 agcttccggc gacatacaga taagaggaac ggtttgaaca tagacactcg cgaaatcacc
     3121 ctggagccag cagacaacgc gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc
     3181 aagcagctcg aacgccgtct cggcatcgag atcaatcgcc gcgacaacca ctttaaactg
     3241 accggccgtc cgatttgcgt caccgctgcg gcagacattc tgcgtagcct gtatgtcgat
     3301 acagccccga tgcgcggtca gattcaggat atcgaaccgg aacagatcca ccttgcgatt
     3361 aaagaagcgc gagtactgga gcaaagtgcg gagagcgtgc cggagtacgg caaagcggtc
     3421 aatatcaaaa ccaaacgcgg cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc
     3481 gccaatattc tcgaccatga cattaccttc ggcgttggcc cggcgggtac gggtaaaacc
     3541 tacctggcag tggctgcggc agttgatgcc ctggagcgtc aggagattcg ccgtattctg
     3601 ctgactcgtc cggcggtcga agccggtgag aaactgggct tcctgcctgg cgatttaagc
     3661 cagaaagtag acccgtatct gcgcccgctg tacgacgcgc tgtttgaaat gctgggcttt
     3721 gagaaagttg agaaactgat tgagcgcaac gttattgaag tcgcgccgct ggcctatatg
     3781 cgtggtcgta cgctgaacga cgcgtttatc attctcgatg agagccagaa caccaccatc
     3841 gaacagatga agatgttcct gacccgtatc ggttttaact caaaagcggt tatcaccggc
     3901 gacgtcacac agatcgacct gccgcgtaat actaaatcag gcttacgtca cgccatcgaa
     3961 gtgctggccg atgtcgaaga gatcagcttt aacttcttcc acagcgaaga cgtggttcgt
     4021 cacccggtgg tggcgcgtat cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa
     4081 cgaaaagcgg cgctggcggc agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg
     4141 tgatcctcga tttacaactg gcatgtgaag ataattccgg gttaccggaa gagagccagt
     4201 ttcaaacatg gctgaatgcg gtgatcccgc agtttcagga agaatcggaa gtgactattc
     4261 gcgtggtcga taccgccgaa agccacagtc tgaatctgac ctatcgcggt aaggataagc
     4321 cgaccaacgt gctctccttc ccgtttgaag tgccgcctgg catggaaatg tcgctactgg
     4381 gcgatctggt tatctgccgt caggtggttg agaaggaagc tcaggagcaa ggcaaaccac
     4441 tggaggcgca ctgggcgcat atggtggtgc acggcagtct gcatttgtta ggttacgatc
     4501 acatcgaaga tgacgaagca gaagaaatgg aagccctcga aacagagatt atgcttgctc
     4561 tgggctatga ggatccgtac attgccgaga aagaataatt tgccaggcat ttgactggca
     4621 gacgtccccg ccgttggtga ttaacgacgg cgttttgatt aactaacatg agaacccata
     4681 cgacgccatg agcgacgaca attcacacag tagtgacacg ataagcaaca agaagggatt
     4741 tttctccctg ttactcagcc aacttttcca cggtgaaccg aaaaaccgtg acgaactgct
     4801 ggcgctgatc cgtgattccg ggcagaacga ccttatcgac gaagatacgc gcgatatgct
     4861 cgaaggggtg atggacatcg cagaccaacg cgttcgcgac atcatgatcc cccgctccca
     4921 gatgattacc ctgaaacgca accagacgct ggacgaatgc cttgatgtca tcatcgagtc
     4981 cgcccactca cgtttcccgg tgattagcga agacaaagat cacattgaag ggattctgat
     5041 ggcgaaagac ttgctaccgt ttatgcgcag cgatgctgaa gccttcagca tggacaaagt
     5101 gttacgtcag gcggttgtcg ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt
     5161 tcgctctcag cgttaccaca tggcgatcgt tattgacgaa ttcggtgggg tttccggtct
     5221 ggtgaccatt gaagacatcc tggaactgat tgttggtgag attgaagacg agtatgacga
     5281 agaagatgat atcgacttcc gtcagctgag tcgtcatacc tggaccgtgc gcgcactggc
     5341 ttccattgaa gacttcaacg aagcgttcgg cacccacttt agcgatgaag aagtcgacac
     5401 tatcggtggt ctggtgatgc aggcgtttgg gcatcttccg gcgcgtggcg aaactatcga
     5461 catcgacggt taccagttca aagtggcgat ggccgatagt cggcgtatta ttcaggttca
     5521 tgtcaaaatc ccggatgact caccccagcc aaagctggat gaataaaacc gaaactggat
     5581 agataactac atggcttttg cctcattaat tgaacgccag cgcattcgcc tgctgctggc
     5641 gttattattc ggtgcctgcg gaacgctggc cttctctcct tacgatgtct ggcctgcggc
     5701 gattgtttcg ctgatggggc ttcaggcgct gacctttaac cgccgcccgc tccagtctgc
     5761 cgctattggc ttctgctggg gatttggcct ctttggcacc ggtattaact gggtctatgt
     5821 cagcatcgcg acctttggcg ggatgcctgg cccggttaac atcttcctgg tggtgctgct
     5881 ggcggcgtat ttgtcgctgt ataccggact gtttgctggc gtgctgtcgc gcctgtggcc
     5941 gaaaaccacc tggctgcgcg tggcgattgc cgcccctgct ctctggcaag tgaccgagtt
     6001 tctgcgcggt tgggtgctga ctggcttccc gtggttacag ttcggctata gccagattga
     6061 tggcccgtta aaagggctgg caccaataat gggcgtggaa gccattaact tcctgctgat
     6121 gatggttagc ggcctgctgg cactggcgtt agtcaaacgc aactggcgtc cgctggtggt
     6181 ggccgtcgtg ctgtttgccc ttcccttccc gctgcgttac atccaatggt ttaccccgca
     6241 accggagaaa accattcagg tttcgatggt tcagggcgat attccgcaat cactgaaatg
     6301 ggacggagac cagcttctta atacgctgaa gatttactac aacgcaacgg caccgctgct
     6361 gggcaaatca tcgttgatta tctggccgga gtcggcgata accgatctgg aaattaatca
     6421 gcaaccgttc ctcaaagcac tggacggtga gttgcgtgat aaaggtagct cgctggtaac
     6481 cgggattgtc gacgcgcgtc tcaataagca gaaccgctac gatacctaca acaccatcat
     6541 cacgctgggt aaaggtgcac cgtacagcta cgaatcagcc gatcgctata acaaaaacca
     6601 tctggtgccg tttggcgagt ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt
     6661 ctttgatctg ccgatgtcgt cgttcagccg tgggccatat atccagccgc cgctgtcggt
     6721 aaatggtatt gagcttactg cggccatttg ctacgagatc attcttggcg agcaagtgcg
     6781 cgataacttc cgcccggata ccgactatct gctgactatc tccaacgatg cgtggtttgg
     6841 taagtctatt ggtccatggc agcacttcca gatggcgcga atgcgtgcgc tggagctggc
     6901 gcgcccactg ttgcgcagca ccaacaacgg cattacggcg gtgattggcc cgcagggtga
     6961 gattcaggcg atgatcccgc agttcacccg cgaggtgttg accactaatg tgacaccgac
     7021 caccggactc acaccgtatg cacgtaccgg caactggccg ctgtgggtgc tgacggcgct
     7081 gtttggtttt gccgctgtgc tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg
     7141 tatcctgcat gagcaaaacg tactttctca ttgagctttg gggcatggtt atcatgcccc
     7201 tttttcttac ggttcaggga tacaagccac attgccatat tggggatgac gaataattgc
     7261 ctgcggatca agcgagcgcg tcctgtccat ttggatcaac ccgttcttcg ccatgacaaa
     7321 tatttttcta ccatccgctg aaaaacgaag gtgattaacg tagctggcag gataaacgcc
     7381 aataatctgc ccggcattaa tattgagcag gatgacataa ccttgcaggc catatgacac
     7441 caacgctaaa tattgaccgt ccgggctaat accctcaacg gaataaacgc tcctgctaca
     7501 ccattgcgtc ataatgctgg cttgttgatc ccctacgctt atacgtgaaa tcccctgact
     7561 atcggcaaaa ataacctcgc cgttttcagg tcgaaaacgc atcgccgata tcgttccacg
     7621 cgcatcacct gcgggaaagt cttctttctg atgcgtgatg gtattgatcc gcacgatgtt
     7681 acctctgcct gctcccggaa ccagtaatgt tctttcatcc ggggaaagtg ccataaccca
     7741 ggcgcctggt ttaccgctcg tccccgtccc gtcgtatttc tccagcggga tatccgccac
     7801 atgctgataa gtcaccagat caatcgctga cacactatta ctggtgcgat tcatcaccca
     7861 ggcttttgtg ccatcacgcg agaggataaa actgtcagga tgtgcaccgg tggtaattga
     7921 ctgaataatc tcccctgtcg ccacgttaat gaccgataac gcattcgctt caaatgacgt
     7981 cgcataaatc aacttttcat ccgggcttaa tgccagcgat ctgacgccct gatctttaca
     8041 gccaccacaa tgtttgactg acttttcttt cgctatgggc gctgtaaagg tatgcaggat
     8101 tttcttttgt ccgaggtcga aaacaacaat gctgttggtg ttttcaattg cggcatacaa
     8161 cgtattacca tcccgactga tcgccatatc atggatatcg gcaatctcag cgagtaaatc
     8221 accactggcc tgttcacgac ttaccggagc ggataatgtt tccctgaggg cgttgcgttc
     8281 ctccgctaac tgttgttttt tttgtcgtcg ctcttgttca atctcctgcg gataaaacgc
     8341 ttcacgcagg ctttgcctga cctgtaaaac tgactcatcc atctgctgct gcgcaagcca
     8401 gatagccccg gtcagaagaa gactgcccgc aacaataatc ccgccttccg taacgagtct
     8461 gttttgccca gataaacgcg cgctcagcca acccaacgcg ccacccgcaa tcaggccgcc
     8521 aatgtggcaa gcgttatcta ttcctgtttg ccgggcaccg tacaacagtg tcagcgccac
     8581 catacccaac agaggaaata ctcgccgctg gtttttatgg tatgtgcccg tacctgaagc
     8641 aaactgtgtt gctatcgctg caccagctat tcccagaatc gccccggatg ccccaacgct
     8701 gatgactatc tgctcacttt cgcgtaacgc ataacaggcg ctgatgagcc caccaaagac
     8761 gccggagaat aaccaggtaa tcaggagtcg ccatttcccc agaatctgtt ccgccacgcc
     8821 tcccacgacc aggaatgcaa acatattcat cagcagatga gaaacactac tgtgcagaaa
     8881 gatactgctc aataagcgcc agggttcacc cgacaaagta agaggcgcaa cgtttgcacc
     8941 gagatggata agtgcggatt gatcgacctc ttcataactc atccattgtg cgagataaca
     9001 aaatatccca atacagagtg caaaaaggat aagggttatt gcgggtaatt gaacatttaa
     9061 cggcttaacc gaagatgcag acataaggtg tttcttcctt gaaaagacag gtaaaaagca
     9121 tttttcccgt gtgcagttat catcccggca aacgtcccat gccgaaaaga taattctgtc
     9181 cgaaatatga ctttttcagc ctaaaattac aatcgtaatt aattcgcccc taagtatttt
     9241 ttggcatgta agttgcttta tttactgtgt aagcgatgaa atcagccgcg acgcaaccag
     9301 gttgcaccac cacaaatcag cggtagcacc aaaaaggtgc ggtttaagaa ttttgcttcg
     9361 gaatggtgcg gcgcatctcg caaaaacaaa caataaaaca gcaatatctt tacatttagc
     9421 caaactaaat gttacctaac aggcacaaca ctgcacaata aagttgcaga cgataacaac
     9481 acaaacactc acaacgggta tccatgcgtt cttaacgcag aagataaagg agttggatat
     9541 gcaattacgt aaacctgcca cagcaatcct cgccctggcg ctttccgcag gactggcaca
     9601 ggcagatgac gccgccccgg tagcgggcag tacgctggac aaaatcgcca aaaacggcgt
     9661 gattgtcgtc ggtcaccgtg aatcttcagt acctttctct tattacgaca atcagcaaaa
     9721 agtggtgggt tactcgcagg attactccaa cgccattgtt gaagcagtga aaaagaaact
     9781 caacaaaccg gacttgcagg taaaactgat tccgattacc tcacaaaacc gtattccact
     9841 gctgcaaaac ggcactttcg attttgaatg tggttctacc accaacaacg tcgaacgcca
     9901 aaaacaggcg gctttctctg atactatttt cgtggtcggt acgcgcctgc tgaccaaaaa
     9961 gggcggcgat atcaaagatt ttgccgacct gaaaggcaaa gccgtagtcg tcacttccgg
    10021 cactacttct gaagttttgc tcaacaaact gaatgaagag caaaaaatga atatgcgcat
    10081 cattagcgcc aaagatcacg gtgactcttt ccgtaccctg gaaagcggtc gtgccgttgc
    10141 ctttatgatg gatgatgccc tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa
    10201 ctgggatatc gtcggcaagc cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga
    10261 tgatccgcag ttcaaaaagc tgatggatga cactatcgct caggtgcaga cttccggtga
    10321 agcggaaaaa tggtttgata aatggttcaa aaatccaatt ccgccgaaaa acctgaacat
    10381 gaatttcgaa ctgtcagacg aaatgaaagc actgttcaaa gaaccgaatg acaaagcact
    10441 gaactaatta caagaaccag gggcggaaat tccagccctc tcgattgtta cgtagcacgg
    10501 acagactata cgcctgatgg tcgttcccca tcgggcctga aaaccgcaat acgctgggta
    10561 acaatcttcg agggtagcag ttaacgctgc taccctcttt tttctggagt agatttatgt
    10621 ctatagactg gaactggggt atttttttac aacaagcccc gttcggcaac accacctatc
    10681 ttggttggat ctggagtggt tttcaggtga cgatcgcttt gtcgatctgc gcctggatta
    10741 tcgctttcct cgtcggttca tttttcggca ttttacgtac cgttccaaac cgttttctct
    10801 ccggtctggg tacgttgtat gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct
    10861 ttacctggta tctggtgatc ccggagttgc tgccagagaa aatcggtatg tggtttaagg
    10921 ccgagctgga tcccaatatt cagtttttcc tttcatccat gctctgcctg gggctgttta
    10981 ccgccgcccg tgtttgcgaa caggtacgcg ccgccattca gtcgttgccg cgtgggcaaa
    11041 aaaatgccgc gctggcgatg ggcctgacgc taccgcaggc ttatcgatac gtactgctgc
    11101 ctaatgctta tcgcgttatc gtcccgccga tgacttccga gatgatgaac ctggtgaaaa
    11161 actccgccat cgcctcgact attggtctgg tggatatggc ggcgcaggcg ggtaaattgc
    11221 ttgattactc agcccacgcc tgggagtcgt ttaccgctat tacgctggcg tatgtgttga
    11281 ttaacgcggt tatcatgctg gtaatgacgc tggttgaacg taaagttcgc ctgcctggca
    11341 acatgggggg caaataatgt acgagtttga ctggagttcc attgtccctt ccctgccata
    11401 tctgctcgac gggctggtga tcacgctgaa aatcaccgtc acggcagtcg tgattggtat
    11461 tttgtggggc accatgctgg cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt
    11521 tgccaaagcc tacgttaacg tattccgctc gattccttta gttatggttt tgctgtggtt
    11581 ttacctgatc gtgccaggtt ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat
    11641 ccgtttaatt tcggcgatgg tggcgttttc aatgtttgaa gcggcctact attcagagat
    11701 tatccgtgct ggtattcaaa gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg
    11761 aatgactcac tggcagtcga tgaaactgat tattctgccg caggcgttcc gcgcgatggt
    11821 gccgcttctg ctcactcagg gcatcgtact gttccaggat acctcactgg tgtatgtgtt
    11881 atctctggcc gatttcttcc gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt
    11941 tgagatgatc ctatttgccg gatttgttta tttcgttatt agtcttagcg cgtcgttgtt
    12001 ggtcagctac ttgaaaagaa ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg
    12061 tatggtcact ttcaggtgct gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg
    12121 gtggtttgcg gcccgtctgg ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc
    12181 gaaccagtgc agcaaggtga aatcaccgtc gatggtatcg tggttaatga caagaaaacc
    12241 gatctggcaa agctgcgttc ccgcgtcggg atggtgttcc agcattttga gttgttcccc
    12301 catttgtcga ttattgaaaa cctgactctg gcgcaggtga aagtgcttaa acgcgataaa
    12361 gcgcccgctc gtgaaaaagc attgaaattg ctggagcgcg tgggactttc cgcacatgcc
    12421 aataaatttc cggcgcagct ttccggcggt cagcagcagc gtgtggcaat cgctcgcgcg
    12481 ttgtgtatgg atcctattgc gatgctgttc gacgaaccga catcggcgct ggatccggag
    12541 atgatcaacg aagtgctgga cgtgatggtg gaactggcga acgaaggaat gaccatgatg
    12601 gtggtgaccc acgaaatggg ctttgcccgc aaagtggcga atcgggtgat ctttatggac
    12661 gagggtaaaa ttgtcgaaga ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac
    12721 cgcgcgaaag acttcctcgc gaaaatcctg cattaatcac tctggcgcgc atacccaagt
    12781 gcgcgccatc tcacgcttta tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc
    12841 ttgtcgcaaa gaagcacaca acaaggagca acaatggcac tgccaattct gttagattgc
    12901 gacccaggtc atgacgacgc tatcgcaata gttctcgccc tcgcctcacc agagcttgat
    12961 gtcaaagcaa ttacgtcttc cgccggaaac cagacaccag aaaaaacctt acgcaatgtt
    13021 ctgcgtatgc tgaccttgct taatcgcacc gatattccgg tagcaggcgg cgcggtaaaa
    13081 ccgttaatgc gtgagttgat tatcgcggac aatgtgcacg gcgaaagcgg tctggatggc
    13141 ccggcattac cggaaccgac gttcgcaccg caaaactgta cggcggtaga gctgatggcg
    13201 aaaacgctgc gcgaaagtgc ggaacctgtc acgattgtgt ctaccggacc gcaaactaac
    13261 gttgccttgc tgctcaatag ccatccggaa ctgcatagca aaattgcccg tatcgtgatt
    13321 atgggcggcg caatggggct tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt
    13381 gacccggaag cggcagaaat tgtcttccag tcagggatcc cagtggtgat ggccggtctg
    13441 gatgttactc acaaagcaca aatccacgtt gaagacaccg agcgtttccg cgcgattggc
    13501 aaccctgttt caaccattgt tgccgaactg ctggatttct tcctcgaata tcataaagac
    13561 gaaaaatggg gctttgtcgg tgcaccgctg catgacccat gcaccatcgc ctggctgttg
    13621 aaacccgagt tgtttaccac tgttgagcgc tgggttggcg tggaaacaca ggggaaatat
    13681 agccagggta tgacggttgt tgattattat tatctgactg gcaataaacc gaatgccact
    13741 gtaatggtcg atgttgatcg tcagggcttt gttgatttac tggctgatcg tctgaaattt
    13801 tacgcttaac acacgtcatt gattaagatc accacgcgtt acctgggtgg tcttaatctg
    13861 tttatctctt tttctttcaa gatataaatg caatggataa tgcagaactc gccattggta
    13921 tcgatctcgg cactaccaat agtttaattg ccgtctggaa agacggtgcc gcgcaattaa
    13981 ttccaaataa gttcggtgaa tatttaacac catccataat tagcatggat gaaaataatc
    14041 atattttagt cggaaaaccg gctgtatcac ggcgtacttc gcatccggat aaaacggcag
    14101 cgttatttaa acgtgcaatg ggcagtaata ccaactggcg gttaggcagc gacacattta
    14161 acgcgccaga actgtcctct ttggtattac gctcattaaa agaagatgcc gaagaatttc
    14221 tgcaacgtcc gattaaagat gtggtgatct ccgttccggc ttatttcagc gatgaacaac
    14281 gcaagcatac ccgtttagca gcggagttag ccgggttaaa tgcggtacgc ttaattaatg
    14341 aacccacagc agctgcgatg gcgtatggcc tgcataccca acaaaatacc cgttcgctgg
    14401 tgtttgatct cggtggcggc acgtttgacg ttacggtgct tgagtacgcc acgccggtga
    14461 ttgaagttca cgcctccgct ggcgacaact ttcttggtgg cgaagatttt acccatatgc
    14521 tggtcgatga ggttttaaaa cgcgcggatg tcgccaggac cacgcttaac gagagtgaac
    14581 tgggagcctt gtacgcctgt gtggaagcgg caaaatgtag caatcaatcg ccattgcaca
    14641 ttcgctggca gtaccaggat gaaacgcggg agtgcgaatt ttacgagaac gaactggaag
    14701 atttgtggtt gccgctgctc aatcgcttgc gagtgccgat tgaacaggcg ttgcgcgatg
    14761 cacgtctgaa gccgagtcaa atcgacagtc tggtgctggt tggcggcgcg tcacaaatgc
    14821 cgctggtgca gcgaatcgcc gtgcgtctgt ttggcaaatt accgtatcaa agttacgacc
    14881 cgagcaccat tgtcgcgctg ggcgcagcaa tccaggccgc ctgccgctta cgcagtgaag
    14941 atattgaaga ggtgatcctc actgatattt gcccttactc gttgggcgtt gaagttaacc
    15001 gccagggcgt ttccggcatt ttctcgccaa ttattgaacg aaacaccaca gtacccgtgt
    15061 cgcgtgtaga aacttactcg accatgcacc cggaacagaa ttcaattacg gttaacgtct
    15121 atcagggaga aaaccacaaa gttaaaaata acattctggt tgaatccttc gatgtgccgt
    15181 tgaagaaaac cggggcttat cagtcgattg atattcgctt tagttatgat atcaacggct
    15241 tgcttgaagt tgacgtgctt ctggaagatg gcagcgttaa gtccagagtc attaatcata
    15301 gcccggtaac attgagcgcg cagcagattg aagagagtcg gacgcggtta tccgcattga
    15361 aaatttatcc gcgcgatatg ctcatcaacc gcacctttaa ggccaaactg gaagagttgt
    15421 gggcgcgggc gctgggtgac gagcgagaag agattggccg ggtgatcacc gattttgatg
    15481 cggcgttgca gtcaaacgat atggcccgcg tcgatgaggt caggcggcgg gcgagcgttt
    15541 atttagccat tgagacctca taatcacggc tgccggatgc ggcgtaaatg ctgcatccgg
    15601 catcggcgca aaaaacacat cgtcaactaa acaacatcgc gattttgccg agagcataac
    15661 ctataaccag caccgttaaa ctgattttga taatgctggt aatgctctgt ggtttttcac
    15721 cctcatcatt tttttcttca tggttttcag gtgttggctc atcgacatct tcatcctccg
    15781 gctcgagttt tactttcgtc aacagcatcc gggtgataat ggcctctaat ggatgagcat
    15841 ctccgccttc cagccttgta ttaagacggg ccagcatctc ttgtttcgga aacgcttcaa
    15901 gtactacctg ttccgctacc cgatacaaat ccagtaacgg tgaatcttct tctgtctcgc
    15961 taccgactct cgatttccac cacgcccatg ctttgcgttt gaagaataac gactcaataa
    16021 acgattgacc taacggcgat aattttccgc tatgtaaaat ttccgcccat aacagccgcg
    16081 tccgggcgct ctcaccagga acatatttca gcggttgacc ttgcgcatct accatgctcc
    16141 gcgcttcgat aaccatcact aataacgcga aataatccgg gcaatgacgc ttgctccact
    16201 gcaacaacaa attatcagct tgtgtagatg ggtaactttc ccggtacgcg cagagatcgg
    16261 caagcaggct ttccccctcg ccgcagtaca acctttgagc acacaagtaa tatcgggcat
    16321 cttcgctttc tggttcttcc atttgccact gtttggctac aggaatgagt tccgccatac
    16381 ctgtttgcac cgagctgtac cagcgcaaca gtttgcgatg taactttgca tcatcaggaa
    16441 tgaaccacgg accttccatc gccaaaaacg cagtgacgta gtcaggatgg taacggcagc
    16501 aacgttccag cgcgaagtaa tactcaacag tctggttctg caacgcaacg ggatattcca
    16561 gaaggctaag gaaatcgaaa caatcaccag cctgaatggc ctcaagaaaa gcctcccttt
    16621 cttcctcatc cattccttcg ctgtcattaa acgaatgcca gttcaaacgc tctgccagca
    16681 aagaagcaca atttaatgaa agatatcgcg cttctatagc gattgcacac agcggccagc
    16741 gtaattgatc gacatcctcc atgttccagg tatttaattg ctgaataaat ttctgccagg
    16801 cggagggctg aaaacgatca ctttctgaat ccagtaatgt cctgaatgca cgcagtattt
    16861 catgttcagc ggcggcatct ttttcttcat catctgcttc ctcgacagga tttaccgcca
    16921 gtcgcagcgc ctcttcatag gcctggcgta atgctttaaa cccctgcgga tcggtttcgg
    16981 gatgacataa cggtaagcga gcaagataag cctggcggat aatgtctatt tgcgtcgtgc
    17041 tttcaatttc gaggatttgc caacatgttt tcataggtcg ctcacatcct taagcgttac
    17101 cgggcattca ggctggacag ttaatggctg ccagggaagc gtggcataga aacgatcgct
    17161 tcccgctgcc agctcttcga acatccgact cccggcgcta tcgtatttgt acagggttag
    17221 cgcctcgcgt agcgaagtgt tgtcttcgca ctgagaagat tgccagtaga gacgtcctaa
    17281 cgaataggcg gaaaaatagt gcatccagct gtgatagtaa tggtgagctc gtacatagac
    17341 gcacgattgc agccataaac tctcttcttc cgtcagccag ttgttgagca caccgacccg
    17401 tgacagaaac cccattcgaa cataatccca ggctttgatt cccccttctc cacagcacat
    17461 agcggtttgg gcaacgaatt gggtataaat caaccctctc tccgagccac cagcaattaa
    17521 tgctttccac tcttcagggg aagagcgatg ccatgtcaga tataagccag ctaaatcatt
    17581 ggcatgacca tcatcagtca tctgataaat agtctgaatt aaatcatcac gattatttat
    17641 tccccaggat gatgagagat cgaattcttt tatcccggta tacatttttg gcgagtcatg
    17701 cgcgggtttg caaccctgat taagtatttc catgggcgca gaaagcgcaa aaagaagata
    17761 ttgttcttcc ttattcattt gttaataatc ccttaagtgc attaactgac tactttttat
    17821 tattgatgag tataaacttg acgctttcaa aataaaaaga aaatcgaagc attcacatat
    17881 gaataaaaaa ttaatgtata tattcgcaat atttatagtt gcagcaatta cctgtattag
    17941 ccaccccaag aaaacaacgt tgcgtgataa agccatggtg aattatgcct ttgattattt
    18001 aagctcaccg ggcagtctac cgtttaccac ggcggccacg gagctttccg cgattcatgg
    18061 tcactcaacg tcgcaatatc gccttggaga attttatctt catggtagcg acggtaaacc
    18121 actggattat acacaggcga gatactggta tgagcaatca gcagaacagg aaaatccgcg
    18181 cgcacaaagc aaactggggt ggatctacct caaaggtctg ggggtcaaac ccgacacccg
    18241 taaagcaatt ctctggtata aggaagcagc tgaacaaggg tatgctcatg ctcaatatac
    18301 tttaggtttg atctacagaa atggctcagg tattaatgtt aaccattatg aatctcaaaa
    18361 atggttaaaa ctggccgcca aacaacatta caaaaatgcg gaaagattac ttgccgggct
    18421 tcccgcacat taacgtcgac tgtcaaccga acagatggag tattttccct atcaaaccag
    18481 caaagataaa aatatagaaa ataattttaa tgatgcccag acagcctggc ccaccatcgt
    18541 caacgggcat tggctgcggt tgcggctccg gttcaacagt ttgcttctcg gtaaaattcg
    18601 ccgcctgaat taataatcga cgaataatta atgcttccag tggacaacga tcttctgcat
    18661 caaggcggct cacaatcggc attaatgatt cttcggttgg ttggttcgcc tgcactaatt
    18721 gtttggcaac taaatataat ggccataagg gatgttctgt atcacgcgtg agtgagatcc
    18781 gcggcttacg tcctttgtgc aaaaccatct cgaccagtga acgccccaga gcagagagtt
    18841 tttcatcgct gagtatttcg tataaatgaa aacgcgtacg ggcgctgtcg ccggggatat
    18901 aaagtagcgg ttttccctga tcgttaacca gttcacgagc ttcaatcatc atcaccacta
    18961 acgggtaata atcgacccga tgctgacggc accattgcaa tattaaagca tccgcctggg
    19021 tggagggata ctcgcgccag tagtcacaaa gttctgccag caagctttcg ccttctccac
    19081 aatagacgcg ttgtgcgtat tcataataac gcgcatcttc attatcagat tcagcttccc
    19141 gccacgaccg ggcgtaatcc aggagttcgg gaattcccag gcgacctgcg ctataccagc
    19201 ggagtaaatt tctgtgcagt tttgcatcat cggggataat cactgcgcga tgttgcgcta
    19261 aatacaatgt cagccattct ggatgcgatg accaaatccg agcgaacatt tggtaaaaat
    19321 cgatggtgtc attttgcacg gcaacgggca gatgcagaat ggtctggaag ttaaaaacat
    19381 tccccttcgc aatggcatat aaaaaggatt ccagttcttc ttcgtctatt tccccgacgt
    19441 tttcctcttg caaccaccgc aatctttctg ctaataacac cacgcactcg aaggataaat
    19501 gggcagtgtt catggcgatt gtgcacagcg accagcgtaa ttcatcaatc tcttccatcg
    19561 agcaataatt taattgctga atgaatcgct gccaggcgga gggcagaaaa cgttcgctat
    19621 cagaggcaag taacgcacga aaggcgagca gaatttcatg ttctgctacc tcatattctt
    19681 ctggttgcca aacagattta gcaggcgact gcgcaatccg tagcgcctcc tcatacgcct
    19741 gacgaagttg tttaaaaccc tgcggatcgg tttctggatg aaaggacggt aacagcgcca
    19801 gataagcgcg gcggataata tcgacgtcag tcgtttcctc tatttcgaga atcttccagc
    19861 aatttttcat aggtcgctca tatccttaag cgtttccggg catgcagaat agtaagccaa
    19921 tggttgccag gacaaggtag ggtaatacgc atcatcactg gcaaataatt gataaaacat
    19981 atcattgccg gaatcgtcgt attctttacg cgctaagaat tcgcgtaata actgcagatg
    20041 atcatcttcg ggagactgcc aatattgccg accaaaggta taaccggcaa agtattgccg
    20101 ccagttacga taataacgta gagcccgtag atgaatgcgc gactggatcc atagactttc
    20161 ttcttcgctc aaccagttat taagtacgcc cattcggctt aaaaaaccca tccggacata
    20221 atcccaggct ttaattcctc cctcaccaca gcattcggcg gtgctggcga caaattgcgc
    20281 ataagcctga ccttgctcat ttagttcagc aagatagtca cgccattcac atggcgaata
    20341 acgaaaccag cggtgataaa accctgccaa ccgagcggca tgaccgttat cgatcattcg
    20401 gtgaattgtg cgcagcaggt catcacgggc atcaattccc caggaggaag aaaggtcaaa
    20461 aaagcatgta cctaaataca ttttgggtga tgaatgcgaa ggtaaacagc attcattgcg
    20521 tatttccatc ggagtggaaa gtgcaaacaa tattttttgc gactccatgt ccatataatt
    20581 tatccttaaa tattcattaa atgaatatta gccgcattgc gaggctcgtt tatttatact
    20641 tttctgacgc gacataatac gcataaaata aggcttacgc aaaaatggaa cacataaaaa
    20701 tataatatgt tttatctgca accataagga aatgataatg attttcacgt caagttgctg
    20761 cgataattta tcaatagatg agattatcga acgtgctgaa aaaggcgatt gcgaggctca
    20821 atatattgtt gggttttatt ataatcgcga tagcgcaatt gattctccgg acgacgaaaa
    20881 agccttttac tggctgaagc tggccgctga gcaaggtcat tgtgaagcac agtattcctt
    20941 agggcaaaag tataccgagg ataaaagccg tcataaagat aatgagcaag ccatcttctg
    21001 gctgaaaaaa gctgccctac aaggccatac tttcgcttcc aacgcccttg gctggacact
    21061 ggatcgtgga gaagccccca attataaaga agcggttgtc tggtatcaga tagccgcgga
    21121 gagcggaatg tcttatgcgc aaaataatct tgggtggatg tacagaaatg gcaacggagt
    21181 cgcaaaagac tatgcgctgg catttttctg gtacaaacaa gctgcattac aaggccatag
    21241 tgacgcgcaa aacaatctgg ccgatcttta tgaagacgga aaaggcgttg ctcaaaacaa
    21301 gacactcgcc gcattctggt atttgaaaag cgcacagcag ggtaatcggc acgcccagtt
    21361 tcaaattgca tgggattata acgctggcga aggggtggac caggactata agcaagcgat
    21421 gtactggtat ctgaaggctg ccgctcagga aagcgtcggc gcttacgtca acatcggtta
    21481 tatgtataaa cacggacaag gcgttgagaa agattatcag gctgcctttg aatggtttac
    21541 gaaagccgct gaatgcaatg acgccactgc ctggtataac ctggccatta tgtatcacta
    21601 cggagaagga agacctgtcg atctccgaca ggctctcgac ctgtatcgta aagttcagtc
    21661 atccggaacc agagatgtca gtcaggaaat tcgtgagact gaagatttac tgtagagata
    21721 aaaacaaggg ccggggaatt tcattcggcc catattctct ttattgctgt gcgaaatcag
    21781 actattacgg ctcaaacggg cgtctctgga actggtcatg accacatttc gggcatagcg
    21841 tcagcacttc cggtgtgtag atcgggagat ggaagtgaca tttctcgcag accagatttc
    21901 ccagcccgac cacttctccg ctgtgataaa ccccatgatg attgaggtcc tggaaaactt
    21961 cgcgccattc aagctgcgtt ttatcggtga tatctgccag ctcctgccat aagctttctt
    22021 taatcacccg cataaagacg ctgtcagatt cttctttcag gctctcttca tagctcatgg
    22081 cgaactcttc caggtcacgt cttacagctc gcgtcagctc atcgacctcg gttcgcgtta
    22141 actcccctgt ttttattacg cgctcgcgcg cctgttccac cagtgcgtcg atatcacgtt
    22201 cgccattgcg caggcgttcg ctcagtgacg cgaccagttc acggtaatat tgagcaacct
    22261 tgttcatcgt ttcatctccc gggtcgttaa ctgactataa taatagacgt tctttgttcc
    22321 ttgctttgct aatacgccca caaactctgt aaattaatgt atgaataatc cgggcatcat
    22381 ttcaacgtcg agtgcgcgaa aggctgtttt gacgcggacg tttgggctat gctatgcgga
    22441 tctgaaaaac cacctcaacg ctacatttgt agccgtattg aaaacaggac cactggctgc
    22501 catgcaagag caataccgcc cggaagagat agaatccaaa gtacagcttc attgggatga
    22561 gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag aagtattact gcctgtctat
    22621 gcttccctat ccttctggtc gactacacat gggccacgta cgtaactaca ccatcggtga
    22681 cgtgatcgcc cgctaccagc gtatgctggg caaaaacgtc ctgcagccga tcggctggga
    22741 cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac aacaccgctc cggcaccgtg
    22801 gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa atgctgggct ttggttatga
    22861 ctggagccgc gagctggcaa cctgtacgcc ggaatactac cgttgggaac agaaattctt
    22921 caccgagctg tataaaaaag gcctggtata taagaagact tctgcggtca actggtgccc
    22981 gaacgaccag accgtactgg cgaacgaaca agttatcgac ggctgctgct ggcgctgcga
    23041 taccaaagtt gaacgtaaag agatcccgca gtggtttatc aaaatcactg cttacgctga
    23101 cgagctgctc aacgatctgg ataaactgga tcactggcca gacaccgtta aaaccatgca
    23161 gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc ttcaacgtta acgactatga
    23221 caacacgctg accgtttaca ctacccgccc ggacaccttt atgggttgta cctacctggc
    23281 ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa aataatcctg aactggcggc
    23341 ctttattgac gaatgccgta acaccaaagt tgccgaagct gaaatggcga cgatggagaa
    23401 aaaaggcgtc gatactggct ttaaagctgt tcacccatta acgggcgaag aaattcccgt
    23461 ttgggcagca aacttcgtat tgatggagta cggcacgggc gcagttatgg cggttccggg
    23521 tcacgaccag cgcgactacg agtttgcctc taaatacggc ctgaacatca aaccagttat
    23581 cctggctgct gacggctctg agccagatct ttctcagcaa gccctgactg aaaaaggcgt
    23641 gctgttcaac tctggcgaat tcaacggtct tgaccatgaa gcggccttca acgccatcgc
    23701 cgataaactg actgcgatgg gcgttggcga gcgtaaagtg aactaccgcc tgcgcgactg
    23761 gggtgtttct cgtcagcgtt actggggcgc gccgattccg atggtgacgc tggaagacgg
    23821 taccgtaatg ccgaccccgg acgaccagct gccggtgatc ctgccggaag atgtggtgat
    23881 ggacggcatt accagcccga ttaaagcaga tccggagtgg gcaaaaacta ccgttaacgg
    23941 tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt atggagtcct cctggtacta
    24001 tgcgcgctac acttgcccag agtacaaaga aggtatgctg gattccgaag cggctaacta
    24061 ctggctgccg gtggatatct acattggtgg tatcgaacac gccattatgc acctgctcta
    24121 cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg gtgaactctg acgaaccagc
    24181 gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc ttctactatg ttggcgaaaa
    24241 cggcgaacgt aactgggttt ccccggttga tgctatcgtt gaacgtgacg agaaaggccg
    24301 tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt tataccggca tgagcaaaat
    24361 gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg gttgaacgtt acggcgcgga
    24421 caccgttcgt ctgtttatga tgtttgcttc tccggctgat atgactctcg aatggcagga
    24481 atccggtgtg gaaggggcta accgcttcct gaaacgtgtc tggaaactgg tttacgagca
    24541 cacagcaaaa ggtgatgttg cggcactgaa cgttgatgcg ctgactgaag atcagaaagc
    24601 gctgcgtcgc gatgtgcata aaacgatcgc taaagtgacc gatgatatcg gccgtcgtca
    24661 gaccttcaac accgcaattg cggcgattat ggagctgatg aacaaactgg cgaaagcacc
    24721 aaccgatggc gagcaggatc gcgctctgat gcaggaagcg ctgctggccg ttgtccgtat
    24781 gcttaacccg ttcaccccgc acatctgctt cacgctgtgg caggaactga aaggcgaagg
    24841 cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa gcgatggtgg aagactccac
    24901 gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa atcaccgttc cggtggacgc
    24961 aacggaagaa caggttcgcg aacgtgctgg ccaggaacat ctggtagcaa aatatcttga
    25021 tggcgttacc gtgcgtaaag tgatttacgt accaggtaaa ctcctcaatc tggtcgttgg
    25081 ctaagcgcgg gaggaagcgt gcgatatctg gcaacattgt tgttatctct ggctgtgtta
    25141 atcaccgccg ggtgtggctg gcatctgcgt gataccacgc aggttccttc cactatgaag
    25201 gtcatgatcc tggactcagg cgatccgaac gggccattaa gccgtgcggt gcgtaaccag
    25261 ttacgtctga atggtgtcga gttgcttgat aaagaaacca cgcgtaagga cgttccatcc
    25321 ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat cggtattccg taacggtcaa
    25381 acagcagagt atcagatgat catgacggtg aatgcgaccg tgttgatccc cggccgtgat
    25441 atctacccga ttagtgccaa agtcttccgt tcgttctttg ataacccgca aatggcgtta
    25501 gcgaaagata acgaacaaga catgatcgta aaagagatgt acgaccgtgc tgccgaacag
    25561 ctgattcgta agctgccaag catccgtgct gcggatattc gttccgacga agaacagacg
    25621 tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct ccaccacgct gggtaactga
    25681 tgattcggtt gtacccggaa caactccgcg cgcagctcaa tgaagggctg cgcgcggcgt
    25741 atcttttact tggtaacgat cctctgttat tgcaggaaag ccaggacgct gttcgtcagg
    25801 tagctgcggc acaaggattc gaagaacacc acactttttc cattgatccc aacactgact
    25861 ggaatgcgat cttttcgtta tgccaggcta tgagtctgtt tgccagtcga caaacgctat
    25921 tgctgttgtt accagaaaac ggaccgaatg cggcgatcaa tgaacaactt ctcacactca
    25981 ccggacttct gcatgacgac ctgctgttga tcgtccgcgg taataaatta agcaaagcgc
    26041 aagaaaatgc cgcctggttt actgctcttg cgaatcgcag cgtgcaggtg acctgtcaga
    26101 caccggagca ggctcagctt ccccgctggg ttgctgcgcg cgcaaaacag ctcaacttag
    26161 aactggatga cgctgcaaat caggtgctct gctactgtta tgaaggtaac ctgctggcgc
    26221 tggctcaggc actggagcgt ttatcgctgc tctggccaga cggcaaattg acattaccgc
    26281 gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc ttttcattgg gttgatgctt
    26341 tgttgatggg aaaaagtaag cgcgcgttgc atattcttca gcaactgcgt ctggaaggca
    26401 gcgaaccggt tattttgttg cgcacattac aacgtgaact gttgttactg gttaacctga
    26461 aacgccagtc tgcccatacg ccactgcgtg cgttgtttga taagcatcgg gtatggcaga
    26521 accgccgggg catgatgggc gaggcgttaa atcgcttaag tcagacgcag ttacgtcagg
    26581 ccgtgcaact cctgacacga acggaactca ccctcaaaca agattacggt cagtcagtgt
    26641 gggcagagct ggaagggtta tctcttctgt tgtgccataa acccctggcg gacgtattta
    26701 tcgacggttg atatgaaatc tttacaggct ctgtttggcg gcacctttga tccggtgcac
    26761 tatggtcatc ttaaacccgt ggaaacgctg gcgaatttga ttggtctgac gcgggtcaca
    26821 atcatcccta ataatgttcc tccgcatcgt ccccagccgg aagcgaacag cgtgcagcgt
    26881 aaacacatgc ttgaactggc gattgccgac aagccattat ttactcttga tgaacgcgag
    26941 ctaaagcgca atgccccctc ttacactgcg caaacactga aagagtggcg gcaggaacaa
    27001 gggccggacg tgccgctggc gtttattatt ggtcaggatt cattgctgac ctttccgacc
    27061 tggtacgaat acgaaacgat actcgacaat gcacatttga tcgtctgtcg gcgtccaggt
    27121 tacccacttg aaatggcgca accgcaatac cagcaatggc tggaagatca tttgacacat
    27181 aacccggaag atcttcacct tcagcctgcc ggtaaaattt atctggctga aacgccgtgg
    27241 tttaacatct cggcgaccat catccgcgaa cgtttgcaaa acggtgaatc gtgtgaggat
    27301 ttattgccgg aaccggtact gacttacatt aaccaacaag gcttgtatcg ctgatacctg
    27361 tcgttacgga gggcatgatg cgactgtggt taattcgtca tggtgaaacg caagcgaata
    27421 tcgatggtct ttacagcggt catgcgccca cccccctgac cgcgcgcggt attgagcaag
    27481 cgcaaaatct gcatacactg ctagatgatg tttcctttga cctggtttta tgcagtgaac
    27541 tggaacgggc acagcatacc gcgcgacggg ttctcagtga ccgccagctc cccgtgcaaa
    27601 tcatccctga actcaacgaa atgttttttg gcgactggga gatgcgacat catcgcgacc
    27661 tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa tgactggcag catgctatcc
    27721 ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt ggaaagattt atcgcaaggc
    27781 ttagtgaata tcagcactat cagaatattt taatcgtcag ccatcagggc gtactgagtc
    27841 tgttaattgc tcgtctaatt ggcatgcctg ccgaatctat gtggcatttt cgcgttgacc
    27901 aggggtgttg gagcgccatt gatatcaacc aaaaattcgc gacgctacgc gtcctcaata
    27961 gccgtgccat cggggtcgag aatgcatgac ttttctgttt ttttacgggt aagccgcaac
    28021 gaccattgac agccccgggc aggctgatat tctccgcagc cagacttttt ccgccagaca
    28081 cgactttgta gaaattgttt tacaaaaatg gcgatgcaat ctgcggcgcg gggtgggatg
    28141 atagcccact ttcgaaagcc gattcggcga caattgtccc gaaatcgcct ctggttcagg
    28201 tatactgaca gaccattttt atctatttga ttcacccagg gggaaaactt gcagggtaaa
    28261 gcactccagg attttgttat cgacaaaatt gatgacctca aaggtcagga catcatcgcc
    28321 ttagacgttc agggcaaatc cagcatcacc gactgcatga tcatctgtac gggtacgtcc
    28381 agccgtcatg ttatgtccat tgctgaccac gttgtgcagg agtctcgcgc agcgggcctg
    28441 ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga ttgtcgtgga tttgggcgat
    28501 gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt atgaactgga aaaactctgg
    28561 agttaatgcg tgaagctgca acttgtcgcc gtgggaacga aaatgccgga ctgggtacaa
    28621 accggtttta ccgagtacct gcgtcgtttt ccgaaagata tgcccttcga gctgattgaa
    28681 attccggccg gaaaacgcgg caagaatgcg gacatcaagc gcatactcga caaagagggt
    28741 gagcagatgt tggcggccgc aggcaaaaac cgcattgtca ccctcgatat tccaggcaag
    28801 ccctgggata cgccgcagtt agccgctgag ctggaacgct ggaagctgga tggtcgcgac
    28861 gtcagtctac tgattggcgg gcctgaaggg ttgtcgcctg cctgtaaagc ggcggcggag
    28921 cagagctggt cgctgtcggc gcttaccctc ccccatccgc tggttcgcgt gctggtcgca
    28981 gagagtctgt accgggcgtg gagcatcacc accaaccatc cttatcaccg tgagtgataa
    29041 aggagctttg agtagaaaac gcagcggatg aaactacaga actcttttcg cgactatacg
    29101 gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct ttttggggat tttgctgctg
    29161 accggcgtgc ttatcgccaa cctgtataat ctgcaaattg ttcgctttac cgattaccag
    29221 acccgctcta atgaaaaccg cattaagctg gtgcctatcg cgcccagccg cggcattatc
    29281 tacgaccgta acggtatccc tctggcactc aaccgcacta tctaccagat agaaatgatg
    29341 ccggagaaag tcgataacgt gcagcaaacg ctggacgctt tgcgcagcgt ggttgatctg
    29401 accgatgacg atattgctgc attccgaaaa gagcgcgcac gttcacaccg tttcacctct
    29461 attcccgtga aaactaacct gaccgaagta caagtagctc gctttgccgt caatcagtac
    29521 cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt actatcctta cggttcggcg
    29581 ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata aagacgtcga acgcctgaat
    29641 aatgacggca aactggccaa ctatgcggca acgcatgata tcggtaagct gggcattgag
    29701 cgttactatg aagacgtgct gcacggtcag accggttatg aagaggttga agttaacaac
    29761 cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc aagccggaca cgatatttac
    29821 ctgacgctgg atctcaaact ccagcaatat attgaaacgc tgctggcggg tagccgcgca
    29881 gctgtggtag tcaccgatcc gcgtacaggt ggggtgctgg cactggtttc cacgcctagt
    29941 tatgacccaa acctgtttgt tgacggtatc tccagcaaag attattccgc cttgctgaac
    30001 gacccgaata caccgctggt gaaccgcgcc acacaggggg tttatcctcc tgcgtctaca
    30061 gttaaaccct atgtggcggt ttcggcatta agcgccgggg tgatcacgcg caatacgacg
    30121 ctgtttgacc caggctggtg gcaactacca gggtcggaaa aacgttatcg tgactggaaa
    30181 aaatggggcc acgggcgtct gaatgtcaca agatcgctgg aagaatctgc ggataccttc
    30241 ttctatcagg tggcctacga tatggggatc gatcgcctct ccgaatggat gggtaaattc
    30301 ggttatggtc attacaccgg tatcgacctg gcggaagaac gttccggcaa catgcctacc
    30361 cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc agggtgacac cattccggtt
    30421 ggtatcggcc agggttactg gacagcgacc ccaatccaga tgagtaaggc actgatgatc
    30481 ctgattaatg acggtatcgt gaaggttccg catttgctga tgagcaccgc cgaagacggc
    30541 aaacaggtgc catgggtaca accgcatgaa ccgcccgtcg gcgatattca ttccggttac
    30601 tgggagctgg cgaaagacgg tatgtacggt gttgctaacc gccctaacgg tacggcgcat
    30661 aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat ctggtaccgc tcaggtcttc
    30721 ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg ccgagcgttt acgtgaccac
    30781 aaactgatga ccgcctttgc gccatacaac aatccgcaag tggctgtcgc catgattctg
    30841 gagaacggcg gcgcgggtcc ggcggttggt acgctgatgc gccagatcct cgaccacatt
    30901 atgctgggtg ataacaatac cgatctgcct gcggaaaatc ctgcggttgc cgcagcggag
    30961 gaccattaat catgacggat aatccgaata aaaaaacatt ctgggataaa gtccatctcg
    31021 atcccacaat gctgctgatc ttactggcat tgctggttta cagcgccctg gttatctgga
    31081 gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat aggccaaatc gcgatgggtc
    31141 tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt ttatgaaggc tgggccccct
    31201 atctctatat catctgtatt attttgctgg tggcggtaga tgctttcggt gccatctcta
    31261 aaggcgctca acgctggctg gacctcggta ttgttcgttt tcagccgtcg gaaattgcca
    31321 aaatagccgt accactgatg gttgcgcgct ttatcaaccg cgacgtttgc ccgccatcgt
    31381 tgaagaacac tggcatcgcg ctggtgctga tatttatgcc cacgctgctg gtggctgcac
    31441 agcctgacct gggaacatca atcctcgttg cgctttccgg tctgtttgta ctgttcctct
    31501 ctggccttag ctggcgtctg attggcgtcg cagtagtgct ggtggcggcg ttcattccta
    31561 ttctgtggtt cttcctgatg catgattacc agcgccagcg cgtaatgatg ctcctggacc
    31621 cggaatcaga cccactcggc gcgggctatc acattattca gtctaaaatt gctattggct
    31681 ccggcggatt acgcggcaaa ggctggctgc atggcactca gtcacaactt gaatttctac
    31741 ccgaacgcca taccgacttt atcttcgcgg tactggcgga agagctggga ttagtgggca
    31801 ttctgattct gctcgctctc tacattctgc tgatcatgcg cgggctgtgg atagccgcca
    31861 gagcgcaaac cacctttggt cgcgtcatgg ctggcggctt aatgctgata ttattcgttt
    31921 atgtcttcgt aaatattggt atggtaagcg gtattctgcc ggttgtaggg gttccgctcc
    31981 cactggtcag ttatggagga tcagcgctaa ttgtgctgat ggctgggttc gggattgtaa
    32041 tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt gtaagaggtg cgcaatgcgt
    32101 aagcagtggc tcgggatctg catcgcggca ggaatgctcg cggcatgtac aagcgatgat
    32161 ggtcagcaac agacggtaag tgtaccgcag cctgcggtat gtaacggccc catagttgaa
    32221 attagcgggg cggacccgcg tttcgaacca ctgaacgcga cggcaaatca ggattaccag
    32281 cgcgacggta aaagctacaa aatcgtgcag gatccgtctc gatttagcca ggcgggactg
    32341 gcggcaatct atgatgccga accaggcagt aacctgacgg cctctggcga agctttcgat
    32401 ccgacacagt tgacggcggc ccatccaacg cttccgatcc ccagctacgc cagaatcact
    32461 aacctggcta acgggcgaat gatcgtggtg cgcattaatg atcgcggccc ctacggcaac
    32521 gaccgcgtaa tttcgctttc tcgcgcagca gctgaccgtc ttaacacgtc aaacaacacc
    32581 aaagttcgta tcgatccgat tattgtcgcc caggatggtt cgctttctgg tcctggtatg
    32641 gcttgtacca cagtcgccaa acagacttac gccctgcctg cacctcccga tttaagcggt
    32701 ggcgcggggg caagttcagt gtctggcccg cagggtgaca ttcttccggt cagtaactcg
    32761 acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca gtagcggttt cctcggcgct
    32821 ccaacgacct tagcgcctgg tgtactggaa agcagcgaac cgacacctgc accacagccc
    32881 gttgttacag ctccgtcgac aacgcctgca acctcgcctg caatggtgac accgcaagcc
    32941 gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg gggccgttag cgatcaggct
    33001 cgtgcgcaac agtaccaaca gcaactggga cagaagttcg gcgtccccgg tcgcgtaact
    33061 caaaatggcg cggtctggcg tatccagctt ggcccattcg ccagtaaagc cgaagccagt
    33121 accttgcagc aacgtttgca aaccgaagcc caattacagt catttattac caccgcgcag
    33181 tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat gacgttaaca aagtcacgcg
    33241 gaaagtcaga tgcctgccgg tagtgcattt gctatagtag ggcacttttt ttaattccat
    33301 cacggatgtc gtagttcaga ccatgaatac cattttttcc gctcgtatca tgaagcgcct
    33361 ggcgctcacc acggctcttt gcacagcctt tatctctgct gcacatgccg atgacctgaa
    33421 tatcaaaact atgatcccgg gtgtaccgca gatcgatgcg gagtcctaca tcctgattga
    33481 ctataactcc ggcaaagtgc tcgccgaaca gaacgcagat gtccgccgcg atcctgctag
    33541 cctgaccaaa atgatgacca gttacgttat cggccaggca atgaaagccg gtaaatttaa
    33601 agaaactgat ttagtcacta tcggcaacga cgcatgggcc accggtaacc cggtgtttaa
    33661 aggttcttcg ctgatgttcc tcaaaccggg catgcaggtt ccggtttctc agctgatccg
    33721 cggtattaac ctgcaatcgg gtaacgatgc ttgcgtcgcc atggccgatt ttgccgctgg
    33781 tagccaggac gcttttgttg gcttgatgaa cagctacgtt aacgccctgg gcctgaaaaa
    33841 cacccacttc cagacggtac atggtctgga tgctgatggt cagtacagct ccgcgcgaga
    33901 tatggcgctg atcggccagg cattgatccg tgacgtaccg aatgaatact cgatctataa
    33961 agaaaaagaa tttacgttta acggtattcg ccagctgaac cgtaacggcc tgttatggga
    34021 taacagcctg aatgtcgacg gcatcaaaac cggacacact gacaaagcag gttacaacct
    34081 tgttgcttct gcgactgaag gccagatgcg cttgatttct gcggtgatgg gcggacgtac
    34141 ttttaaaggc cgtgaagccg aaagtaaaaa actgctaacc tggggcttcc gtttcttcga
    34201 aaccgttaac ccactgaaag taggtaaaga gttcgcctct gaaccggttt ggtttggtga
    34261 ttctgatcgc gcttcgttag gggttgataa agacgtgtac ctgaccattc cacgtggtcg
    34321 catgaaagat ctgaaagcca gctatgtgct gaacagcagt gaattgcatg cgccgctgca
    34381 aaagaatcag gtcgtcggaa ctatcaactt ccagcttgat ggcaaaacga tcgagcaacg
    34441 cccgctggtt gtgttgcaag aaatcccgga aggtaacttc ttcggcaaaa tcattgatta
    34501 cattaaatta atgttccatc actggtttgg ttaaaaattg aacacttgaa agtgtaattt
    34561 ccgtccccat atactaagca tcagtaaaaa aactcccgcc ttctggcggg agttgctatt
    34621 taattacgtt acgccggagc tgacatgaaa accaaactta acgaactgct tgaattccct
    34681 actcctttta cttacaaagt tatggggcag gcgttacctg agctggttga tcaggtggtt
    34741 gaagtggtac agcgccatgc gccaggtgac tacaccccaa cggtaaaacc aagcagcaaa
    34801 ggcaactacc actcggtatc tatcactatc aacgccactc atatcgagca ggttgaaaca
    34861 ctgtatgaag aactgggcaa aatcgatatt gtccgcatgg ttctgtaact cgcttctccg
    34921 ttacccggct cctggtcggg taactcccct ctcctcgctg tgatatactt ttcccccact
    34981 tttactcatt ctccacggag atgccgtttt gtatcaggat aaaattcttg tccgccagct
    35041 cggtcttcag ccttacgagc caatctccca ggctatgcat gaattcaccg atacccgcga
    35101 tgatagtacc cttgatgaaa tctggctggt cgagcactat ccggtattca cccaaggtca
    35161 ggcaggaaaa gcggagcaca ttttaatgcc gggtgatatt ccggtgatcc agagcgatcg
    35221 cggtgggcag gtgacttatc acgggccggg acaacaggtg atgtatgtgt tgcttaacct
    35281 gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg cttgagcaaa cagtggtgaa
    35341 taccctggct gaactgggta tagaagcgca tcctcgggct gacgcgcctg gtgtctatgt
    35401 cggggaaaag aaaatttgct cactgggttt acgaattcga cgcggttgtt cattccacgg
    35461 tctggcatta aacgtcaata tggatctttc accattttta cgtattaatc cttgtgggta
    35521 tgccggaatg gaaatggcta aaatatcaca atggaaaccc gaagcgacga ctaataatat
    35581 tgctccacgt ttactggaaa atattttagc gctactaaac aatccggact tcgaatatat
    35641 taccgcttaa ttccatacat caatggccca atttacactg ggtcattact cataatacct
    35701 gctaatgctt tacatctcct tcgcgtttgt atattttctt tatcacttgc agaaatgcgg
    35761 tgccacgcct tgctccgcaa ctcaccccat tgattaacct attgaaatag ttgattatgg
    35821 tgagcaaaag cgttccgttc caattagaca taagattatt gtaatttttt aaatattatt
    35881 tttccatgaa taaaattgga gtaagtgcgt ggatagtaat aatcaaattg aaccctgttt
    35941 aagcagaaag tcgtcagaag gtaaaccaca aatatttaca accttacgaa atattgatct
    36001 taaccttctg actatttttg aagctgtata tgtacataaa gggatcgtta atgcagcgaa
    36061 agtgcttaat ctgaccccct cggcaatcag tcagtctatt cagaaactgc gcgttatatt
    36121 ccctgaccca ttgtttattc gcaaaggcca gggtgtcact cctaccgcat ttgcgatgca
    36181 tctacatgag tatatcagtc agggccttga gtccattctt ggcgcgctgg atatcgaagg
    36241 aagctatgat aagcaacgaa cgataactat tgcgaccact ccctcggtcg gagccctggt
    36301 ccttcctgtc atctatcggg cgattaaaac tcactatccg cagcttttac tgcgcaaccc
    36361 gcccatcagc gacgcggaaa accaactcag tcagtttcaa accgatctca tcatcgataa
    36421 catgttttgc accaatcgta cggtgcaaca tcatgttctg ttcaccgaca atatggtgtt
    36481 aatttgccgc gagggaaatc cactactctc tttagaagat gacagagaga ctatcgacaa
    36541 cgctgcgcat gtacttctgt taccggaaga gcaaaatttc agcggtctgc ggcagagagt
    36601 tcaagaaatg tttccggacc ggcaaatcaa tttcaccagc tacaacattt tgacaatcgc
    36661 tgcactggtt gccaacagtg acatgttagc gattattccc agccgttttt ataacctgtt
    36721 tagccgctgc tggccgctgg aaaaactgcc ttttccgtcc ttaaatgagg agcaaataga
    36781 tttctctatc cactacaaca aattcagcct gcgtgatccc atcctgcacg gagtaataga
    36841 tgttatccgt aatgcatttt gaaaaagtat tgttacagcc agatcacagc gcagaacgat
    36901 aaaatggcac aatccaacaa caattttaca ttttcgcgac cgctttggct gctttcaggt
    36961 ccgtttcaat gatatactgc cagtcgttaa ttcaaaaata gttgataatt acaacaatct
    37021 attgaattga aacgctttcc ttcgtaattc gcaactggaa cacgcacgct atgagtaaac
    37081 ccattgtgat ggaacgcggt gttaaatacc gcgatgccga taagatggcc cttatcccgg
    37141 ttaaaaacgt ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg atgaaaatca
    37201 agcttccagc ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc aaaaatggcc
    37261 tgcattctgt ctgcgaggaa gcctcctgcc ctaacctggc ggaatgcttc aaccacggca
    37321 cagcaacgtt tatgatcctc ggcgctattt gtacccgccg ttgtccgttc tgtgatgttg
    37381 cccacggtcg cccggtagct cctgatgcca atgaaccagt gaaactggcg cagaccattg
    37441 ccgatatggc gctgcgttat gtggttatca cctccgttga ccgtgatgac ctgcgcgatg
    37501 gcggtgccca gcactttgcg gattgcatta ctgccattcg ggaaaaaagc ccgcaaatca
    37561 aaattgaaac tctggtgccg gatttccgtg gtcgtatgga tcgtgctctg gatattctga
    37621 ctgcaacgcc accagatgtg ttcaaccata acctggaaaa cgtaccgcgt atttaccgtc
    37681 aggtacggcc tggtgcagat tacaactggt cgctgaagct gctggaacgc tttaaagaag
    37741 cgcatccgga aatcccgacc aagtctggtc tgatggtggg actgggtgaa accaatgaag
    37801 aaattattga ggtaatgcgc gacctgcgcc gtcatggtgt gacgatgtta acgctggggc
    37861 aatatttgca gccaagccgc catcacctgc cggttcaacg ttacgttagc ccggatgagt
    37921 tcgacgaaat gaaagccgaa gcgctggcga tgggctttac ccatgctgca tgcggtccgt
    37981 ttgtccgctc ttcttaccac gccgatttgc aggcgaaagg gatggaagtt aagtaatcct
    38041 gtaataatta cttacatctg tctgataaaa aacccgcttt ttgaagcggg ttttttgtat
    38101 cgaacaagat attgagggag cgtcctgctc gccacgtcac tctttatgag agagcttttc
    38161 agcctgaaga tcaacgtctg cgcctttttt cgccgcagca tcgtcatcat tcatcgcctt
    38221 cttgaaccct ttaatggccg ctccaaggtc tccgcccagc gtacgtaact tcttagtccc
    38281 aaacagcaga acgaccagcg ccgcaactac tagcagtttg gtaatactaa tctcacccat
    38341 agataccttc ttgacatata accggcaaca gaacgcgcct taaaaaccag agaatattaa
    38401 cggtcattcg acgcgcgcac acaatagcaa tctgtaacaa ggtgaatcaa gcgccgagta
    38461 aaaaaacatc ataataattg cggcggcgca aagcgacggt tgtttaagac gggcagttgc
    38521 gcgcgcacct gacgcacacg ttcgggcgtc acttccgcca taatgagtgc aggcatttct
    38581 gacgctgccg caatggtgac gccaaaggga tctataatcc ggctttgacc gatatttttg
    38641 ttcccgcact cccccgccgc caccatataa caggtggtat ccagcgcacg agcggcaagc
    38701 aacgttgacc aatgatgctc tttgagcggc ccgcgaaccc aggcggcagg aagtaccagg
    38761 atttcagctc cctgtaatgc ctgtgccagc gccagctctg gaaagcgtaa gtcataacag
    38821 gtcatcagac cgaccttcat cccctccacc tcgagtaacg gagcgatttc attaccagca
    38881 tcaacacggc gtgattcctg aatggcaaat gcatcataga gatgcaattt ggcataacgg
    38941 gcgacgatgt ttcctgcctg tagcgccacc agcatattcc atgcgcgccc cggcgttgaa
    39001 ggaacatgaa tcgtcagaat tgtcgtcatc atgttacgtt tactttctcg ccgtaaacgt
    39061 ccgaggaatt cgccttccag cagctgtgct gatttaaccg atagatctgc atcatgatca
    39121 tcgcgcgcca gcaatgcttc cggcaggacg aacagcgata cgtcgttttc cgccgcctgc
    39181 gccatcaacg aggcacaaat ctcagcgttc ttttcccaca cagatgtaac agcaaactgt
    39241 cctgcggcaa ccagcattgc cacctcctgt cagagtcgta aaattcgcca acgacgatta
    39301 ttcggcgtta cacttatcac tcatacaaat caaatagcag gattttgcag tgttacaact
    39361 tcttttagca gtttttattg gcggtggtac gggaagcgtg gcgagatggc tgttaagtat
    39421 gcgatttaac ccgctgcatc aggcgattcc gttggggacg ctggcagcaa acctgattgg
    39481 ggcattcatc ataggaatgg gattcgcctg gttcagcagg atgacgaaca ttgatccagt
    39541 gtggaaagta ttaatcacca ccggattttg tggcggtcta acaaccttct caacattttc
    39601 ggcagaagtg gtgtttttgt tacaagaggg ccgctttggc tgggcgttac tgaacgtttt
    39661 cgtcaacctt ctggggtctt ttgccatgac agcactggct ttctggctgt tttcggcctc
    39721 aaccgcacac taaagaccaa aaaaaaaccc gctgattaag cgggttttga attcttgctg
    39781 acgtatctta cagagcgatt acgtttgcag cagaagggcc tttggcaccg ttagtgattt
    39841 cgaactctac gcgctgacct tcagcaagag ttttaaaacc attagtctgg attgcagaga
    39901 agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat gaaaccgaat cctttggact
    39961 cattaaacca cttaacgtta cctttaatct tagacatcaa aattaccttt acatgaaaaa
    40021 tagacacaaa tgctgtgtcg gttaccagta caccaattgt ggtacgcttt gtccagtgca
    40081 actttactaa aaagtgataa aagtcgcgtt atttttgatg ttacttattt tagctattga
    40141 ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg aaagcgcatc caggcaaagt
    40201 acacattgcc attgttgtag gtacccggaa tgtaggtcat ctgaaaagtc actgggccat
    40261 aacccacgga ggccaatggc agtagaaccg ggagagggat gtaattccag ttatcgcgtg
    40321 ccgttacgcc agcggtgaat cccagaccta aatgaaaatt ttcatccgcc agcggtcgcc
    40381 aggtactttc ccatccgtat ccggcaatcg gttcccattt gttccacgag tccttaaatg
    40441 ccatggcata caggccatgc cagtttcctt tttcatccca acgcgacagg ccaaaaccgc
    40501 caccccacgg tcgctcgtta tagcgatcgg ttttttcttt gtcgtaagcg aaacgtgcat
    40561 gccaggtgat ggcaggaata tataaatcat aatgttcagg ctgttgccag gtttgtgcaa
    40621 tattttctct aaacgttgtc atccactcat ctgcgttagc aaaaacttta ccaacgctga
    40681 ttaactgaat aaaaacaaag gaaaagatag cgacatattt actcacgttc atttgtgacc
    40741 ataaaacatt tatcaaaaat ctactactag catagcaaag ctacaattaa cataacctta
    40801 atagacccaa cataaagaat aatctgaata gctgctctat ttatttccgc ttgttcaagt
    40861 taaatatttc cgcgcatcga ttaaagatga tgcgtcgttt tctgcgatgg gaatagtcaa
    40921 aaaagaaaaa ccaagtcttt tttgatgaca aaatgcaatc aaggaaaaat tattttattt
    40981 tttaactaga tacaacaatc atcttaacga agtatataat ataaatcgtc aacccatgct
    41041 catgttagat tattttttga gcgcacagcc gcagctaaca ccattgcaat taacaaattt
    41101 gcatcaatcc accatcaatt tacacacatt attatgtgat aattgccaac cgctaaatat
    41161 gcgttttgtt atctatgtat aaaaacagca acttcaatgt cttaatggca gtttttcttg
    41221 attttaatca gcattcatcg ccaatttatt gggcatattt tttccttaag ctttaggaat
    41281 tttttattat ttactttggg g
//
LOCUS       DAZGOB010000034        40457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGOB010000034 DAZGOB010000000
VERSION     DAZGOB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.034"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(12..590)
                     /locus_tag="ACHLIF_001829"
     CDS             complement(12..590)
                     /locus_tag="ACHLIF_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9605861.1"
                     /translation="MTFRMSEQSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGNSLFISELGPLPENVTWLSP
                     YGEYQKWNGTSWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVNTAVAADVEWPVAPQ"
     gene            complement(590..1444)
                     /locus_tag="ACHLIF_001830"
     CDS             complement(590..1444)
                     /locus_tag="ACHLIF_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9605862.1"
                     /translation="MQGATGLELYEAYNNGYPTAYGNIIHLKGMTAVGEGELLIGWSG
                     ISGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPEEFYPVGAPIPWPSDTVPSGYALM
                     QGQTFDKSAYPKLAVAYPSGMIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASAS
                     STDLGTKTTSSFDYGTKTTSSFDYGTKTTNSAGAHSHNIPVGHTGAGNGVSAGYNAAL
                     GTGTTSSAGGHAHNVYIGAHNHTIGIGAHAHSVIIGPHGHTITVNATGNAENTVKNIA
                     FNYIVRLA"
     gene            complement(1961..2722)
                     /locus_tag="ACHLIF_001831"
                     /pseudo
     CDS             complement(1961..2722)
                     /locus_tag="ACHLIF_001831"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(2725..3255)
                     /locus_tag="ACHLIF_001832"
     CDS             complement(2725..3255)
                     /locus_tag="ACHLIF_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO9605863.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGARITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            complement(3248..4144)
                     /locus_tag="ACHLIF_001833"
     CDS             complement(3248..4144)
                     /locus_tag="ACHLIF_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO9605864.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHGSGTNLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(4148..4498)
                     /locus_tag="ACHLIF_001834"
     CDS             complement(4148..4498)
                     /locus_tag="ACHLIF_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO9605865.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSARDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            complement(4495..5061)
                     /locus_tag="ACHLIF_001835"
     CDS             complement(4495..5061)
                     /locus_tag="ACHLIF_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO9605866.1"
                     /translation="MRLLSNIIRTGIISEVDEESWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVAIACIGGNPETAMIIGSLWSDASPAPGKSLKEIVVSAPDGAVFSY
                     DADAGALRASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     DFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(5073..5708)
                     /locus_tag="ACHLIF_001836"
     CDS             complement(5073..5708)
                     /locus_tag="ACHLIF_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO9605867.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTMGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(5701..6168)
                     /locus_tag="ACHLIF_001837"
     CDS             complement(5701..6168)
                     /locus_tag="ACHLIF_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9605868.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRNSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(6306..6713)
                     /locus_tag="ACHLIF_001838"
     CDS             complement(6306..6713)
                     /locus_tag="ACHLIF_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO9605869.1"
                     /translation="MKLWPTLGVAFLLIAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDAHFT
                     DRLRRHAERATASAVSPAYTTDADHTGNASPLP"
     gene            complement(6710..7102)
                     /locus_tag="ACHLIF_001839"
     CDS             complement(6710..7102)
                     /locus_tag="ACHLIF_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO9605870.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(7099..7422)
                     /locus_tag="ACHLIF_001840"
     CDS             complement(7099..7422)
                     /locus_tag="ACHLIF_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO9605871.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(7425..7625)
                     /locus_tag="ACHLIF_001841"
     CDS             complement(7425..7625)
                     /locus_tag="ACHLIF_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO9605872.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(7625..8119)
                     /locus_tag="ACHLIF_001842"
     CDS             complement(7625..8119)
                     /locus_tag="ACHLIF_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO9605873.1"
                     /translation="MSMVARTEPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTHELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(8224..9024)
                     /gene="gpM"
                     /locus_tag="ACHLIF_001843"
     CDS             complement(8224..9024)
                     /gene="gpM"
                     /locus_tag="ACHLIF_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO9605874.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLTDADSMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAGASEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            complement(9070..10122)
                     /locus_tag="ACHLIF_001844"
     CDS             complement(9070..10122)
                     /locus_tag="ACHLIF_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO9605875.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPSVQQR
                     FENAVKESTEFTKKINVFGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWAAHPDFQSRVSTQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRAIG
                     GLDVFLAPFFPDATMLITSFNNLSIYWQKGTMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            complement(10146..10982)
                     /locus_tag="ACHLIF_001845"
     CDS             complement(10146..10982)
                     /locus_tag="ACHLIF_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO9605876.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSVQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            11137..12888
                     /locus_tag="ACHLIF_001846"
     CDS             11137..12888
                     /locus_tag="ACHLIF_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO9605877.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATATGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            12888..13934
                     /locus_tag="ACHLIF_001847"
     CDS             12888..13934
                     /locus_tag="ACHLIF_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9605878.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKGAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLASHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNSYALLDEKTAP"
     gene            complement(13949..14473)
                     /locus_tag="ACHLIF_001848"
     CDS             complement(13949..14473)
                     /locus_tag="ACHLIF_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605879.1"
                     /translation="MVINYKQLREKREQVKESFRRNEDLTPLVHLAQGIVDAYEISLE
                     LPSQTWTDSDGNRQHYVSCGLETTEGFRRMPLSQIPAATPKARGGNDERKLIFSIETV
                     VDDTPGEVAFVHTPLSIAMYNDEIQVRVNNNIVPLKEGNSPYTTVCEAIQYYVLSEID
                     NLKPDGTQKMVQLW"
     gene            complement(15033..15440)
                     /locus_tag="ACHLIF_001849"
     CDS             complement(15033..15440)
                     /locus_tag="ACHLIF_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605880.1"
                     /translation="MNRKQKQELKYFLRKEIARLEDAESQSSEIPFGMDINDARMLQA
                     YRIAQAALQTKPLNNIHEKYDYFTDTACLLTSEEQKIAQLLGDAWNLYLTLPVEHPMG
                     RDEFCRAIHHCQNMMLARPAIRTLARKGQGYKR"
     gene            complement(15539..15850)
                     /locus_tag="ACHLIF_001850"
     CDS             complement(15539..15850)
                     /locus_tag="ACHLIF_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO9605881.1"
                     /translation="MTTPTRRISFYLKPAAVKNESEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTECPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            complement(15855..16814)
                     /locus_tag="ACHLIF_001851"
                     /pseudo
     CDS             complement(15855..16814)
                     /locus_tag="ACHLIF_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
     gene            complement(16891..19713)
                     /locus_tag="ACHLIF_001852"
     CDS             complement(16891..19713)
                     /locus_tag="ACHLIF_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO9605882.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAHRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDSTDGRWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDRAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTKQPEERPAPWPQLPDGVDVDEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVACYLTQLGASD
                     PEAAARQASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            complement(19720..20085)
                     /locus_tag="ACHLIF_001853"
     CDS             complement(19720..20085)
                     /locus_tag="ACHLIF_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605883.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            complement(20082..20201)
                     /locus_tag="ACHLIF_001854"
                     /pseudo
     CDS             complement(20082..20201)
                     /locus_tag="ACHLIF_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20227..20472)
                     /locus_tag="ACHLIF_001855"
     CDS             complement(20227..20472)
                     /locus_tag="ACHLIF_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO9605884.1"
                     /translation="MTILDYIAANPGCSSGEIAAALNTPTTTINAELRRLWRSGLVTR
                     KERKTGGRFSYQVNLMPFGCSNPLTQMFNQLLREIRA"
     gene            complement(20469..20672)
                     /locus_tag="ACHLIF_001856"
     CDS             complement(20469..20672)
                     /locus_tag="ACHLIF_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605885.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVVQVNLIRQVQESRR"
     gene            complement(20759..20872)
                     /locus_tag="ACHLIF_001857"
     CDS             complement(20759..20872)
                     /locus_tag="ACHLIF_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605886.1"
                     /translation="MSIFISWLVLIISVVCAIGIMRIINSVKKIERFFTEE"
     gene            complement(20869..21111)
                     /locus_tag="ACHLIF_001858"
     CDS             complement(20869..21111)
                     /locus_tag="ACHLIF_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO9605887.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            complement(21123..21410)
                     /locus_tag="ACHLIF_001859"
     CDS             complement(21123..21410)
                     /locus_tag="ACHLIF_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605888.1"
                     /translation="MTKDHAQGVFIRFIDFRGELLLRASAIDGVTPAGKNGADEATYV
                     YLNGTRLLVELPYQTVREIISEAEKARQVNGDEPYIEFICMDSEAEIQKAD"
     gene            complement(21421..21759)
                     /locus_tag="ACHLIF_001860"
     CDS             complement(21421..21759)
                     /locus_tag="ACHLIF_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO9605889.1"
                     /translation="MLKQRRNFCTGTERHANRFTTSASRSNIRYSLSDTHATPDGYPV
                     KQIGEHAWLIEKAGIVIHKCPRNPFTGNRIFALSCGDNQFGQDFTLYEALRTVDRLLR
                     GQSFIKQADL"
     gene            complement(21774..22052)
                     /locus_tag="ACHLIF_001861"
     CDS             complement(21774..22052)
                     /locus_tag="ACHLIF_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9605890.1"
                     /translation="MAKVLNTYEQADFERLAAFYPYRNEHGLPVLEESLEDYAKRTNQ
                     SILAVRRQADRSVIPVTQEGRNTKRKVNLFAIFLKTIRNAEKYVQMTK"
     gene            22148..22459
                     /locus_tag="ACHLIF_001862"
     CDS             22148..22459
                     /locus_tag="ACHLIF_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9605891.1"
                     /translation="MSIEISKKLKAIRESEGLSQAKFADSIGIAVGTVKQYETGIRGV
                     GTEVLLKITMHPEFKKYTTWLMSNETNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            22548..23483
                     /locus_tag="ACHLIF_001863"
     CDS             22548..23483
                     /locus_tag="ACHLIF_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9605892.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYPLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPIAG"
     gene            23494..23889
                     /locus_tag="ACHLIF_001864"
     CDS             23494..23889
                     /locus_tag="ACHLIF_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605893.1"
                     /translation="MEKTVNSDLHLGQYWRIDIVCDPELRDEVEQYFSLHDVGFSKIE
                     VFSVENPYKLALFFDFAKKGVEVAKAIMGLLDRNDIEITMYRATDSSPQSVKSIKLRK
                     SEDVEKCKDLLSTCVAIGVQRNKEKEGSE"
     gene            24079..25059
                     /gene="btuC"
                     /locus_tag="ACHLIF_001865"
     CDS             24079..25059
                     /gene="btuC"
                     /locus_tag="ACHLIF_001865"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO9605894.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            25122..25673
                     /gene="btuE"
                     /locus_tag="ACHLIF_001866"
     CDS             25122..25673
                     /gene="btuE"
                     /locus_tag="ACHLIF_001866"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO9605895.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            25673..26422
                     /gene="btuD"
                     /locus_tag="ACHLIF_001867"
     CDS             25673..26422
                     /gene="btuD"
                     /locus_tag="ACHLIF_001867"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO9605896.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            26500..26964
                     /gene="nlpC"
                     /locus_tag="ACHLIF_001868"
     CDS             26500..26964
                     /gene="nlpC"
                     /locus_tag="ACHLIF_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO9605897.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            27211..27924
                     /gene="ydiV"
                     /locus_tag="ACHLIF_001869"
     CDS             27211..27924
                     /gene="ydiV"
                     /locus_tag="ACHLIF_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO9605898.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            27987..29423
                     /gene="selO"
                     /locus_tag="ACHLIF_001870"
     CDS             27987..29423
                     /gene="selO"
                     /locus_tag="ACHLIF_001870"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO9605899.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(29427..29618)
                     /gene="hemP"
                     /locus_tag="ACHLIF_001871"
     CDS             complement(29427..29618)
                     /gene="hemP"
                     /locus_tag="ACHLIF_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO9605900.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(29750..30796)
                     /gene="aroH"
                     /locus_tag="ACHLIF_001872"
     CDS             complement(29750..30796)
                     /gene="aroH"
                     /locus_tag="ACHLIF_001872"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO9605901.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLEMARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNSRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(30953..31786)
                     /gene="ppsR"
                     /locus_tag="ACHLIF_001873"
     CDS             complement(30953..31786)
                     /gene="ppsR"
                     /locus_tag="ACHLIF_001873"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO9605902.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKLLDIMGLSRRM
                     Y"
     gene            32119..34497
                     /gene="ppsA"
                     /locus_tag="ACHLIF_001874"
     CDS             32119..34497
                     /gene="ppsA"
                     /locus_tag="ACHLIF_001874"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO9605903.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(34554..36200)
                     /gene="fadK"
                     /locus_tag="ACHLIF_001875"
     CDS             complement(34554..36200)
                     /gene="fadK"
                     /locus_tag="ACHLIF_001875"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO9605904.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(36257..36550)
                     /gene="ydiT"
                     /locus_tag="ACHLIF_001876"
     CDS             complement(36257..36550)
                     /gene="ydiT"
                     /locus_tag="ACHLIF_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO9605905.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(36547..37836)
                     /gene="ydiS"
                     /locus_tag="ACHLIF_001877"
     CDS             complement(36547..37836)
                     /gene="ydiS"
                     /locus_tag="ACHLIF_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9605906.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCIMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(37892..38830)
                     /gene="ydiR"
                     /locus_tag="ACHLIF_001878"
     CDS             complement(37892..38830)
                     /gene="ydiR"
                     /locus_tag="ACHLIF_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO9605907.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(38850..39614)
                     /gene="ydiQ"
                     /locus_tag="ACHLIF_001879"
     CDS             complement(38850..39614)
                     /gene="ydiQ"
                     /locus_tag="ACHLIF_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO9605908.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            39930..>40457
                     /locus_tag="ACHLIF_001880"
     CDS             39930..>40457
                     /locus_tag="ACHLIF_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO9605909.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYI"
BASE COUNT         9032 a        10400 c        10724 g        10301 t
ORIGIN      
        1 gctttttctc tttattgtgg ggcgactggc cactcaacat ccgctgctac cgctgtatta
       61 acacgattca acaatacccg gtatgtcttc catgcagcca gtaacgatgc ctcttcctcc
      121 gttgcaatat ccaaatctac ggcatcctga agtggcgcaa tatgctcgct ggctacctgc
      181 atcaggctgt tttttgtttc ttccgcctcc cttatccgaa acagtttttc tgcttctgca
      241 tctttcaccc aggatgtgcc gttccacttc tgatactctc catacggcga caaccaggta
      301 acattttccg gtaatggacc gagttcagaa ataaataacg agttccctga tgccacgtca
      361 taaaccgttt taccccgatg gtcttcaacg agatgccacg atgcctcatc actgttgaaa
      421 acggccacga agccagccgg aatatctggc ggggcaatat cggtactgtt tgcaggcaga
      481 cctgtatgag gtggaatgta tgcatcacct tcaccaataa actcattggt tccggtcagt
      541 agattataaa tttttatggt ccgtgattgt tcactcattc tgaatgtcat tatgcaagcc
      601 tcacaatata gttaaatgcg atgtttttta cggtgttttc tgcgttcccc gtagcattaa
      661 cggtgatggt gtgtccgtgg ggaccaataa tgacagaatg ggcatgagca ccaatgccga
      721 tagtgtggtt atgggcaccg atatatacat tgtgagcatg ccctcctgcg ctcgacgtgg
      781 taccagtacc taacgcagcg ttataaccag ctgatacacc attccccgcg ccagtgtgac
      841 caacaggtat attgtgtgaa tgagctcctg cgctatttgt ggtttttgtg ccgtaatcaa
      901 atgaactggt cgttttagta ccgtaatcaa acgacgatgt ggttttcgtc cccaaatcag
      961 tactggatgc gctggcgctg tgggtgtgcg atttaatgcc gtcctgttcc tgagacaata
     1021 cggcacgacc actggcaggt ttgcccttga tagtccagcc acgcatatca gggatcatgc
     1081 ctgacggata agcgactgca agtttcgggt atgcagattt gtcaaaagtc tgcccctgca
     1141 tcagggcata accagacgga acggtatctg atggccacgg aatcggtgca ccgactggat
     1201 aaaactcttc aggaggatga gccgaggtgt aaagctgcgc ccacggcgac cagtttgcgt
     1261 cggtcgtatc ccgtcgtgaa cgaataaatg ccggagcatg agcaccgctt ataccactcc
     1321 agccgatgag taattcgcct tcgccaacgg ctgtcatccc tttcaggtga atgatatttc
     1381 catacgctgt tggatatccg ttgttatacg cctcgtataa ctccagacct gtggccccct
     1441 gcatattatc tgtcagggcg gtcagccgac cttttgaagc cagattaact gacgatactg
     1501 ctgtcccatc tgacggtaac gccccgatct ctgatgctgt tggcttattc ctggagttat
     1561 agtcccttcg ccagccagga gcataagcat caccatgatt aatataagta aattgagcat
     1621 tagggatgcc ttcaccgctg gatgtactcg gtgtggtaat gcgtatggtc attgcgccgc
     1681 gggtgccaat aacttccaca acagcacctg caagacaaat atttccgcaa cctgtatctg
     1741 taatgacctt attacttgca taagcccatg agcctttgca catccagtaa ggatggttaa
     1801 atgccccctg actctctagc caaaaaatca actgcgcagt tgtccatgct tcactatctc
     1861 caccaatatt cagctctgca ctatatgcac ggcaggcacc gatatttttc gtaaaggtgt
     1921 ctttgtcaga gatatcctca ccattcttag ctttctgcag acgtttttca gcattgtcat
     1981 aggcagcttt tactgcattt gatgttgccg ccagcgtttc agacgtgctg ttggttgcac
     2041 tgctgagctg gactatccct ttttgtgccg tagtggcatc ctgcgctgta tattttccgt
     2101 tagcaagatc atacgcgacc ttaaccgctt tcgtcgttgc agccagcgtt tcagaatcgc
     2161 tgttagtggc gctactgagt tgaacaaagc cttttgcggt cagcgaggca tccgggtgac
     2221 gtcgtgactg ttcatgctct ttcagtttgt tatccacgta atccactgtg gccatcacca
     2281 tggtgttatc cacggtaagc gccacagtgg cagtgctgga tacggtcaga atggtgcgaa
     2341 atgtttgtgt acgaccggac ccttcggcaa cggctggctt gtaactttcg gcagtattgc
     2401 ccaccgcgat taaatcgccg tgctcatcaa acacaccaat ttcccggatc cagaatccgc
     2461 ccgtttctgg aggaataacc agctctgcaa taatgcggtt ctgatgtgtt gcgtccagga
     2521 tgacgcgatt aacagtatgt cgccacacct catgcaccag acgggtctgc ttactgtctg
     2581 gtgtgggcaa tgtaccgcca ccgtcgccca cggccatatg agtcaggcgg acaggcttac
     2641 catctggcgc ggctgcctga gctaattttt tggcacccgt atcggtgata acggttttaa
     2701 atttacgtgt tgtggtactc atgcttaatc gtccggataa atggtaatga cttcgccgtc
     2761 gtaagttgct gccgccgcga aaatatctcc ctgaatctcc tgaatgatat tcagccctgt
     2821 catgtggcgg ctgaccgggc gggcatcagc aatcaaccgc tccatttcca gatacatttc
     2881 ctccgtcacg ccactgtcca gcgtgccgac ttcaacagta aatgttcccg gttctccgcc
     2941 gaactcccac cactcagaca cgcgaatgag gtatcccagc ggctcaatgg cccggcgcag
     3001 tgcgctgatg gtccctttgt gtcggtgtat cagccatgca tcacgaatca cctgtcgctt
     3061 tgtctcttcc ggccagttgc gatcccagcg gtcaacggaa aatgcccagg cgagataagg
     3121 cagcagatgc accgggcagg tgtccggcga ccacagcgtg ttgaggtcta ccggaatgtc
     3181 tgtaatgcgc gctccgacag cttcggcgca acgcatgaaa ctgctggctg atggcggtaa
     3241 cagtgaatta ctcattacgc ccaccttcgc tgatggtgaa tgactcacag cgcgccgcct
     3301 gtatgtcgct gatggccata ttctgtgtgg gttcgattat ctccacgcgt tgcacaccgt
     3361 gcacatgcag tgcggcagca atggcggaca acgccacgtc ctgaccgata agcccctgct
     3421 cagccagcca cttcctgaac gacgattcag ccgcagccag aataggttcg gattccgggc
     3481 cgggataaaa gtacagtttt gcattcagcc gccatgtcac gattcttgca ctctgtacgg
     3541 tcaggcggtc ggccaccggg cgggtatcct ctgcattcag aacggcgcga acggtattaa
     3601 gcaacgcctc cgttgctgtg ccgtcgcctt cagtggacag gatggaaacc gtcacatttg
     3661 ccggagacgg actgatagcc cgcgcatcac gcaccagacc gctggcgctg cgggcaaaat
     3721 actcgtatgc acctgacggg ccagcaacac tcaggccgtc atacgcccgc tgcgcccgca
     3781 gtctcagcga ggtgtcgctc tccatcaccg cgtcggtggt atccgttgcc ggagttataa
     3841 ccaggcgctt tgtgttcata ttgcccgcga ggttgtccag gtttgtccct gaaccgtggc
     3901 ttaacataca ggcgcgtgcg ccctcgttaa cccgctggcg taacagcatt tcacgaaacg
     3961 acatggtttg agcgataacg ttaaggggtt ccgactccag ctccagcgcg gcggaaacgg
     4021 cttcacgctg ttcggcggga taggacgcaa tcatcatggc ctttgtgtca gccagaattg
     4081 cttcaaagtc aggctccgcg atgatggcgg gttccggtaa ctgggaaagg tcaacggcgg
     4141 gcatgattta ctccctcagc gtgatggtta actcaacatt ctgcatggtc tgcatgacag
     4201 tgcccgacag cgtcaccccg gcgcggcctc ccgctttcca gacaacgtcg atggcatcca
     4261 gggcaatgcg gggttcccat cgtgtcagcg caatcacggc agcactcatg cattgcagac
     4321 gcgtggtgtt attcatgggt tcgtcaatca aatcaggcac aaggctgcca tattcccgtc
     4381 gcataacccg gctggccagc ggggtggtca ggatgtccct ggctgactgt ttcaggtgct
     4441 ccatatcatt caggtttccc gttccgtccg ggttcattcc tgtgtagcgg gttgtcactg
     4501 cgggcctcct gtcgaatcgc tgccaccttt aacgccaccg tgtttatgcg tatgcactgt
     4561 gatgccgttt gaggtgaaat cgccgccgct gtgcgtgata ttgccgctca tctttcctcc
     4621 ttttgtgacg tcaagcgtcg ctgttctcag aaggtctgtg cattccacga cgggcgtgtc
     4681 cagtgtcacg ctgacggatg cctgcagggt ggctgttttc atgccgctgg ctctcagtgc
     4741 gcctgcgtcc gcgtcgtagc tgaacaccgc gccatccggc gcgctgacca cgatttcttt
     4801 caggcttttg ccgggggccg gactggcatc actccacagg ctgccaatta tcatggcggt
     4861 ttccgggttg ccgccaatgc aggcaattgc cacctgttcg cctggtgatg gcggcagcca
     4921 cacattgaag gctcccgcgc gcgtggtgtt ccagcgcaac cagcctgttt ccagttcgcc
     4981 gctgcgaacg cgcacgcacc aggattcctc atcaacttca gagatgatcc cggtgcggat
     5041 gatgttgctc agcagtcgca tgagttctgc gctcaccgta cagcctccgc aatccggccc
     5101 agcaccgtgt tataaatcag gcgctcatct gcctgactga tgcccaacag ctcacgtacc
     5161 gggtaatcgg tgaaaatgcc cggcgcaacc tgatcgcgct caccgaactg atgaacgcgt
     5221 gcaatacgtg cggccacgcc gctgtaaccc atcgtcacac cagaagcatc tgcacgggct
     5281 ttcaggtagc gggcggtgcg cagttttacg aacatgggga cgcgctttgt gctgtcctgg
     5341 ttgatgcgtc gggtgcgtat ttccagaaaa cggtcgatgt catcccggta aaacgtgcgg
     5401 atattgtttt tatcctcatc ccacccggta atggttcgcc cgtatttccc cgtgtcgtga
     5461 tgccagtttt tcagcgtgcg tgcttcgtta ttccagataa agcgaatgcg ctcctgtatc
     5521 cgggttacgc ggcgtctgcg tggtgtccat gcggtcccgt ccggcgcttt ctgtgaccgg
     5581 atacgtgcct gctgggcgcg gcgtaaatcc tgtgccagct ttctggcgat gttattgatg
     5641 gcctgctgat tcaggctatc gcggatggcc tcaaaggttt catccacgcg ggtgaatgcc
     5701 ttatccatcg ctttcacccc acgtcacatc ctggaataca tgcgaccagt cgccttcgga
     5761 agagggcagg cggggttttg gctccggcag gtgttctgcc tgcggtgtcc cctgactgtt
     5821 gcgcgtaatg cgaacgcgtt cccgcagggg gagcgtaaac aggagatcgg cgctgtcatc
     5881 gtcattgata acggcggaaa atttgatgtc ctgattacgc tccggattga gcaacaactg
     5941 tggctgattt tcggataacc acgccagcag cggcagcgtg aggtcgtcca gctctccggc
     6001 gtaatccatg acaaacatca ccatctgata gcggtaaaca aacgagggcg tttctccggt
     6061 cgtttcaatg ttgccgctct ccacgaaaat ggtgaatttt tccgggttag cctgacacca
     6121 tcggcatgaa cgggtcatgg cttcacgcag ggaatcagtt ttcagcatgg ttgttatcct
     6181 tgttgttcag tcgttgcagc ctgcgctgtt ccagtaattc aatggcccgt ttatccgagt
     6241 tacaggtttc aagtgcatcc agaaggcggt cgccccatat accgagattt ccccatgtgg
     6301 gagtatcagg gaagggggga ggcattaccg gtatggtcag cgtctgtggt ataagccgga
     6361 ctgacggcgc tggccgtggc gcgttctgcg tgcctgcgca acctgtcagt aaaatgagcg
     6421 tcaggcaaag cgtgggcgca ttcatctttt gcaatatcgt tgcgtagctg ttcacgtctt
     6481 acctctccgt cctgattgcg ttgctgattt tccacgcgga gttgcgccag cacctgctgc
     6541 atatcctgta ccccggcgct gatgatattc agggtgtcgg cggtactttt cagggtgctg
     6601 gcctgcgctt cgtttctggc gttctcccgg cccagcgacc acgacagacg catggatgtt
     6661 ccccatccgg caatcagaag gaaagcgacg ccaagcgtgg gccagagctt catgccggat
     6721 aggctccgtg tggtaactga aaatgaggtc cgtctttcag ggtcttccag tcgccgcccc
     6781 attccaccgg aatattcagt tcccggctgg cctgtctgaa tgctgctgcg attttttcgt
     6841 acagcggcca ttcccatgac acctggctgc cgatataagc cacaacatcc acggcatgcc
     6901 ccgtaaggtg gcggctgttc atggtctggc tcttacccgt ggccaccagt tgcttctggc
     6961 ggtaacggct gcgcaaccct tcggtgatac caaaatccac ttccgagatt tccagtgccc
     7021 gtcgggtcac tttcaccaga tcaggattta cgccctgcaa attcttttcg ctccggctgc
     7081 tgaatttaaa tgtgttgctc attcgtcctt ctccttcacc ctgcgattaa aggccgcaat
     7141 aaccttgtcg cgtgctttct ctgcacccat aaaaccgatt gatgcgccga taaacgtcac
     7201 ggcatcttca ggaaacccga agaagcgcaa cgacccggcc acggccatgg caagaacgcc
     7261 gcacgccagc gatcccgtta cggtctgaac cagtgtccgt ccgtcataaa gactcatcag
     7321 cgcggaaatg ctgaccgccg cgcctactgc atacaccgtt ggcaggtgat caaagagcca
     7381 cgcaataacc tgctctgtga tccctgtttg aatggtgctc actactactc cccccacaac
     7441 tgaatcattt ctcgtttctt cttctccggc tccggcatct ccacttcctg cccggcgtcc
     7501 agaaatacct gctgacagag tccggggttg gcatccagca ccttttcggt gacgccctgc
     7561 gtcgtgccgt agtaccggaa acagagcgaa tccacggtgt cgccttccag tgccttcact
     7621 ttcatcagca caactccgca aagattcgcg ggcggcacag aatgtcagag atggcccagc
     7681 tcacatcgcg ccacaaatcc gatgtctgta tatccagtgc gtccgcccgg cggtcgccct
     7741 tgtccgttgt gtccgcatcg cggtaacgct ccagaatcag ggcgcgtgtg gcggtataaa
     7801 cagcattgcg ccagtgccag agattgacgc tttctccgtt aattacgggt gccggaacat
     7861 cggccagcgt ctgatgtcca gccgcctgct gttcctgctg ccatgcttcc agctcgtggg
     7921 taacgtgtgc cacagccccg gtggcggtat gcagcaggcg ggaggtggtc acacggcccg
     7981 gcagtcgtac cgccagacgc agctcacgca gcacaatatc cggccagaat gcacccgctg
     8041 aaatacgggt atcaccatca tcggtatcgg tgatgtcgtc ctctgcgggt cctggttcag
     8101 ttctggcaac catactcatg gggttcactc ctgaaaaaaa aatcgggcgg tgggtgcgcg
     8161 gtgtaaacgg tcacggagtc agaccggaac accgcgcacg ccgcccgctg acggggtcag
     8221 tcgttaaccg cgcttcgcct tctgcgtcgc ggtggttttt cgtgttgcag gcttccgcgt
     8281 tgtcttttta cttttgctgc tttcgtcctg cgcctgctgt gcgctggcgt cttctgatgc
     8341 gcctgcggaa tcggcttttt tcagggcgcg ggaaagggtt gcaatctcgc gtttcacacc
     8401 tgcgttcggg ttcaggtgca tcgcttcgcg cagcagcttc agtgatgagg ccatgctgtc
     8461 tgcatcggtc aggccacgac gggcaaaggc acacgccttg cataatttgg cgcgcacttc
     8521 gtccggcatg tcctggttgg cgacaatttc ccaaagtgtg tccagtggtt cgataaaggc
     8581 ggataaatcc gcgtcggcat ccgtcccggc ctgcgtcagt accgggttgc agatttcttc
     8641 ggtcagtacc gtggctgcag tacgtccaaa gttatccggc atgatgaggt tgtgacggac
     8701 cacatatgca ccaatacgca gcgccagcgg aagatcgccg cagtcaatcg cccacaccat
     8761 cagcgtggca atcacttcat cctgctgccc gccgtcagcc tccagcgttc cctcaatcca
     8821 gccggaaaag tccggcaata actctttttt gatggcggct ttcgcgcttc tggcctgtac
     8881 gcccttaagc cgggcctgtg ccagacgcag acgatacagc acctcttcat gcgcggtacg
     8941 cgcggcgtgg tccacgcctt cattcgcccg gcctgcgcgc tgtgccatca cgttctgcca
     9001 gtgttgctgt gcaggagtaa tcattttttc tctccgttac aggcgggcat gatgcccgcc
     9061 gtgagttgat tagctgtcgg cgaacttcag gccagtgacc atcgcgcact tgccatagtc
     9121 ttcaacgaca taagcgtcat tgatggactg gtaggtggcg atgcggttgt attccggctc
     9181 gtctttcatc aggcgacgca ttgttccttt ctgccagtaa attgacaggt tgttgaacga
     9241 ggtgatcagc atcgttgcat ccgggaagaa cggcgcaagg aatacatcca gcccgccaat
     9301 ggcgcgcgat gacaggatga gctgtccggc aagtaattcc gcattgggat tctggccgct
     9361 gatgctgttc agcacgggca gacgcagcga gttaaacagg ttgcgcccca taatcaccac
     9421 gaggtcgtca gcttccttgt gccattcatc cagcagggat gagcgcgcgt cctgtaccag
     9481 tgcatcagcg ttcgcatact tacccgcgtg cgccacggtg ttgtccatgt tgcgggaggt
     9541 cagcgttaca tcattcataa cgcgctcgct ggcgtcggtt ctgatgtgct ccagccatcc
     9601 cacgttaacg tcctgaagca gcttgttagt gctgaagttg gactcatctg cgtgagacgt
     9661 gccgttgaaa ccgatcatga tgcggtcaag cgccacctgc cgggcaatct gtgtgctgac
     9721 gcgggactga aaatcagggt gtgccgccca ggcatcaagc tgcggatacg aaataaacgt
     9781 gtcgtagttc acctgttcgc actggtattt gcggtttttc agatcaacca cgttattcgg
     9841 gttacggcgt tttgtgccgt cataactggt attcgtgcgc gcaatcggcc cggtggtgtc
     9901 caggaggatt ttttcgcctt tctggtcagt cacaccgaac acgttaattt tttttgtaaa
     9961 ttcagtgctc tcctttactg cgttttcaaa acgctgctgc accgagggtt ctacggtaaa
    10021 tcgcgatacc agtgcagata ccgggatatt gttaagcgac gcctgctgcg ccatatagca
    10081 acccagcttg ttgcgggtaa tatctgacat caccagattc ataaaaaatt tgctcctttg
    10141 tcttatcaga agtcagccag ctggtcggag gctgcgcccg ttgcggtgaa ccggttctgc
    10201 ggatcgccgt cctgcgtgcg cagtttttcc ttcagtgctg tcagctctgt ggtcagtgac
    10261 gtaattttct ggcggtcctg ctgatggcgg gtttccagca cattaaaacg gtcgataatg
    10321 tcggcctgtg acgttgcgac gccttccacc gcttcctgaa tacgggaaaa actggcgtca
    10381 tccgctttgc ggccacggcc aataatcccc attacgcggt taaaccactg ggtgccttct
    10441 tcctggcgtt gttctgccat ttcgatgatt tcagactcga tggcttcgga gataagcggc
    10501 gcttcaccct ggacactgtt gaacgtcatt accgcctgac gttgctgtgc cgtgaatttc
    10561 aggcgctcag tgcccaggct tgccggggtg tcggtcatcg ccagcccgac cagataggcg
    10621 cgcccgttaa cggagaactg cgggtgcagt tcgatactgg aatagatttt cttgccgtcc
    10681 gcgacaagct gcttcatgcg ctcggtcggt tcgatttctg catacagcgc agtacgtccg
    10741 gccagcggac cttccgtaat gtcttccgta ctcagtgcgg tgacatcgcc cattgcggaa
    10801 aattcgcttg acgggcatgg cgagagatag tgctcaacgt tcacgcgggc agcgtaaaca
    10861 tccgggttga agttctcggc ggcttcacgc agatgcaccg gactgatttc acggccatca
    10921 acagttgatc cggagacagc cacgcgaaac tttttgcggg atgtcttttt ttcattagcc
    10981 atagtttttg cccctctgac tggttcttca gtcatgatgg caaagcgtaa caggctgata
    11041 caaagggctt ttgttgtaag aaaacggcca gaacaggggg ttaaggagaa cagtttcgcg
    11101 cgcgggtaat cttcctgtaa ttactcaggg ggagcaatga ttcaggacgc ttttgtgcgc
    11161 cagcgtgcgc ggcaacttta ctggcagggt tatccgcccg cagaaatatc acgtctgatg
    11221 ggaataaacc cgaacacgat ttatgcgtgg aaaaaacgcg accagtggga tgaaacgcca
    11281 cccgtgcagc gtgtcacgca gtccatcgat gcgcgcctca tccagcttac tgaaaaacag
    11341 aataaaacag gcggtgactt taaggaaata gacctgctga cccggcagct taaaaaactg
    11401 catgatggcc agccggatgc gacggccaca ggaaagaaag gccgggcgaa aaaactcaaa
    11461 aatcatttca cgccggaaca gattgccgca ctgcgggaaa aaatcatcag caggctggag
    11521 tggcatcagc ggggctggtt tgactccctg accctttgca gggaagccgg gatacgtaac
    11581 aggatgatcc tgaaatcccg acagattggg gcgacctggt attttgcaca ggaagcactg
    11641 ctgatggcgc tgcgtgacga tgtggcgcaa ccttaccagc gtaaccagat ttttttgtct
    11701 gcgtcgcgtc gtcaggcgtt ccagtttaaa agcattattc agaaggccgc ggctgaagtt
    11761 gatgtggagc tgaaaggggg cgataaaatc atcctctcca acggcgcaga gctgcatttt
    11821 ctcggcactt ctgctgcgtc ggcacagtcc tatacgggca atttttattt tgatgaattt
    11881 ttctgggtca gtcgctttgc tgaactgcgc aaggtggctg gcgctatggc aaccctcagc
    11941 ggactgcggc gcacctactt ctccacgcca tccaccgaaa cgcacgaggc atacgcctac
    12001 tggaatggcg accgctggaa cgagaaaaag gcctcgcata aacgccagcg tttttctgtg
    12061 gactggaaaa cgctgcataa cgggcttatc tgccctgacc ggacgtggcg gcaaattgtc
    12121 acgctggaag atgtggttaa tcacggctgg aaacacaccg atatcgacga aattcgtgat
    12181 gaaaacaccg aagacgagtt cctcaatctc tatatgtgtg agtttgtccg cgaaggggaa
    12241 tcggcattta acctgaatat cctgattggc tgcggtgttg acggatacga cgactggaaa
    12301 gactggaaac cttttgctcc ccgaccgatg gggaatcgtc cggtatggat tgggtatgac
    12361 gcaaacggca gtagtggcaa cggcgacagc ggcgctgtgt ccgttgtggt tcctccggct
    12421 gttcctggtg gccgttttcg aacggtggag actcgacgcg ttcaggggct ggagtttgaa
    12481 gagcaggcca gagtcattga agagttcacg tatcgctaca acgtggaaca catcggcatt
    12541 gatgtgacgg gcgggaacgg ggaggctgtt tatcagatag tgaagcggtt tttccctgct
    12601 gctattccgt acaccttcac gctgtcatca aaacggtcgc tggtactgaa aatgctgcaa
    12661 ataatgcgtg ccgggcggtg ggaatacgat cgcgccgaac gcgagctggt cgcggccttt
    12721 aacgccgtgc gtaaggtgaa aacaccgggc ggctttatca cttacgaaac ggaccgagcg
    12781 agggggatca gccacggcga ccttgcgtgg gcaaccatgc ttgctgtcat taacgaaccg
    12841 attggcggcg aaggagaaaa cgagcgtttc acggttatgg agttctgatg agcagaaaaa
    12901 ataaaaaagt gcgcatgagt tcacgcattg atctcgctga tgcgctcagg aaagaatcat
    12961 cgctcagtgc attcacattt gatggtcctt atcgcctgac cgggcatgac ctgctggata
    13021 atatgtactg tgctgataac gggcggtggt atgaaacccc ggtggactgg tacggtctgg
    13081 caagagctgc ccggcaaacg tcctggcatc agtctgcgct ttactttaag cgcaatgtat
    13141 tactcggttg ctacatcccg cacccgttgc tttcccggca ggatttctcg gcgctggcgc
    13201 tggactggtt tgtgttcggt aacgcattcc ttgagcttcg aagcaatatg ctcggcgaac
    13261 cgcttaaatt acggcacgcc ctggcgaaat acatgcgacg cggaagcgat cttgaatcat
    13321 ggtggtatgt gcaggatggc aagggcgcgt tccagtttcg ccctggcaaa gtgtgccacc
    13381 tgatgaatcc ggacattaac caggaaatct acggcatgcc ggaatatctt ggcgcattac
    13441 tctcggccag cctgtctcat tcggcggaca tgttcagaaa actgtactac gacaacggat
    13501 cccacgccgg gtgcatcatc tacatcggtg cagcgcaggt aaaccgcgaa agcatggact
    13561 ccctgaagga aacgctacag ggggcacgtg gtggtggtgc gtttaaaaac gtgctcattc
    13621 atgcgcccaa cgggggcaaa gagggggtgc aaattttgcc gttccagcag atcaccgcaa
    13681 aggatgagtt catgaatgtt aaggcggcat cccgtgatga tgtgctggct tcgcaccgcg
    13741 ttccgccgca actgatgggg gcgatgccgg gcgaaaaaag tgcgtttggt gatgtggaga
    13801 aggcagcgcg ggtttacgca attaacgagc tgatgcctgt catggaggcc atgaagcaca
    13861 tcaatgactg gcttggtgaa gaggtgatcc gttttaattc ttatgctctt cttgatgaaa
    13921 aaacagcccc gtgatggggc tgtccttttt accagagttg aaccattttc tgggtgccgt
    13981 caggcttgag attatcaatt tcagagagaa cgtaatattg gatggcttca caaacggtgg
    14041 tgtatggtga attaccttct ttaagtggca cgatattatt attaacgcga acctgtattt
    14101 catcgttata cattgcgatc gaaagaggag tatgcacgaa cgcgacttcg ccaggtgtgt
    14161 cgtcaaccac tgtctcaatg ctgaaaatca gttttcgctc atcattgccg ccccttgctt
    14221 tgggggtggc ggcaggaatc tgagataaag gcattctgcg aaacccttca gttgtttcca
    14281 gtccgcatga aacgtaatgc tggcgattac cgtcgctatc cgtccatgtc tgtgatggca
    14341 gctccagaga gatttcataa gcatcaacaa ttccctgggc aaggtgtaca agcggggtca
    14401 gatcttcatt gcggcgaaaa ctttccttta cctgctctcg tttttctcgt aactgcttgt
    14461 aattaatgac cataaaacag cctccattga tttctttgct cgtattttgc acttatgaag
    14521 tgtggtcggc aaggtgctgc atcactgacg cgcttcgctt gtctgctgct tcgccggggc
    14581 ataaaaaatt tatgccccgg ctctccagct cccgtatcaa tcagataatt tcacgacgtc
    14641 ttccgactta tcgctatcat cgacggccag actcttacac aatcccaccg cgctgactgc
    14701 attttctcgc cgcctcagtg cgattttgac ggccttacct ttcatcccat caaatcagaa
    14761 ccccttacgt ctttttcacg ctcagcgtga gcgatacagc cattctgttg tgtcgctgcg
    14821 acatcgttca gggagtgcta tttaccccct gaaacgcggg ctgttccccc gtcacctgcg
    14881 cgcagaaaaa gcgcgttttt ttgtgcacgc acggatcctt gacggatcca gccgcaacgc
    14941 gggccggaag ggcaaaaagt cgttcaaaaa aattgtgcaa atttgtgcac tattgtgcat
    15001 tgaaataaac gccatggagg agggcgtttt acttatcttt tatagccttg ccccttcctg
    15061 gccagcgtcc tgatggccgg gcgtgccaac atcatatttt ggcaatgatg aattgcccgg
    15121 caaaattcat ctctacccat tggatgctca actggcaatg ttaaatacag gttccatgcg
    15181 tcgcctaaaa gttgggctat cttttgttct tcagatgtca gcaagcaagc ggtatccgta
    15241 aaataatcat acttttcatg tatgttattt aacggttttg tctgtagtgc agcctgagct
    15301 atacggtatg cctgaagcat acgggcgtcg ttgatatcca ttccgaacgg aatttctgat
    15361 gattgtgact ctgcatcttc aagcctggca atttctttac gtaagaagta tttcaactct
    15421 tgtttctgtt ttctgttcat gctttttttc ctttttctct gtcaatgtgt tgcattttgt
    15481 cccccttgtt tatgttcccg gattaaagtc atcagggcgg atgcgccctg atgttgtgtt
    15541 attcgggaaa taatgcccgg atatttccgg ccatctgact ggttatctgt gcggttgata
    15601 ctggctgtga cgcggggcat tctgtcctgg tttgtgtcac tgataacgcc tcatcgtcag
    15661 cccatgcagc cagtcggtaa gcctctgctg gattcatttt cagaagtgcc agcccggcca
    15721 gaaaagccac gcgttggccg cttttgcggg cttctggtgt aaggctgtcc agccaggcgc
    15781 atgcttcgct ttcgttcttg acggccgcag gcttcagata gaaacttatc cgtctggttg
    15841 gtgtcgtcat tggtttactc cttgtccatt gcgtacagcc cattaaccag agcaaactgt
    15901 ggcaccccgt ccgcgatgaa agtcgcatta actccgcagg cttcgcggat agcgggtgcc
    15961 acaatctccg cccctccacc gacaaccatc acccgcccgt aacccgaaaa aaccgccagc
    16021 gcgcggatca cgcgttgttt cagtgtttct tccttttcac gaataaccgc catcaggctg
    16081 gcgtaatgcg cgtcattgtg gatgtgctgg cgcagctagg cttcatcatg gcgatgttcg
    16141 ataatggtat tggcgatgtg gtgactggtg cgcataccgt tagtggccat caccgccagt
    16201 acggcatcgg ccatcagaga aacgcctacg tgtggatcgc aaaacacctg gctgatacct
    16261 gccagttgcc cctgaacctt tgccacatcc agcgtggttc cgcccaaatc cacaatcagc
    16321 agggattcaa acggactcat gtcagccagt gccttaaagc cagccggaat ggattcaggc
    16381 ataacccgca cgttacggat agtgaatgct tcgccgttct ggtactccac cgggcgcata
    16441 acgttcgctt ttttgcggtt gatgttggcc atgtccggct gtgcgtttgt gtcgaaatac
    16501 tcgctcagtg gcagggtgac aaccacatcc acctcctgtg gtgtgatgcc tgatttgacc
    16561 agcgcgtgat gaatggcaat gacattcaca tcgctgtatt ggtattgcgt gtcggtcgtc
    16621 tggacaaagc gatcgctgac cggatcaaaa ccatagcgca cgccatcaag catgtagttc
    16681 gcgggctgcg tgccaccgaa cggcgcagac cattccgact tgaagctgtt cgggctgatg
    16741 gcgttgcggc gttcgccgtt ctcagtccat gccagcttga tgttggtgga gccgtcgtcg
    16801 atacaaattt tcatgtcgct tttccttatg ttgattaatt aatcgtttac gggattctga
    16861 aatcccgttt ttgcctgttt tgtgcgcgct tcatatatcg ctgcgcgttt tttgctcatt
    16921 tacgggattc gtgagtcccg tttctgtctg ttttttgttt ccactggtca ggccaccccg
    16981 cagcaggtct gctttgcggc tggcgcgttc agtggtttca ctgattctct gtgcgtgctc
    17041 tgcgtcgcgg atggcgcgca gcatgtcaga aagcacggta acgggtgttt tcatggtgtt
    17101 ctggtcctgc tgaagtgtgg atgcctggcg tgcggcggct tcagggtctg atgcccccag
    17161 ctgtgtcaga tagcaggcga ccgggttatg gcggatctcc gtgctgctta cgccatgatt
    17221 acggctcagg cgctgccaga gctgcgtgat tcggctgtcc gggcgggtat ccggtttgcg
    17281 tacaatttca aatccctgcg gtgcaatgat gctgccgtca acgtacagac tgccgccccg
    17341 taacaggtgc tgcatctgtt gttcaccgat atgcaggccg agagattcag cagactcccg
    17401 ccattcttta gcgagtaatt cgtggttatc aggcaaaggc cgctgctgtt tgcggctctg
    17461 tgtccagctc tgcatttcat cactgctgtt ttttgcctgt ttgtcacgaa gcgaacgcat
    17521 cagcgcccgg cgttcgtgcc gtttcagtga gcgcatccat tcatccacat caacgccgtc
    17581 agggagctgc ggccacggtg ctggccgttc ttccggctgt tttgtcccgt tgttgtccgt
    17641 ttcctgtaca cggggacagt tattgccacg agtccaaggg gcggcagggc cgccctgaag
    17701 gtcaaaacca ttttcgcggg cgctgtcttc cgcttccggt ttgcgtctta ccagcttcca
    17761 gttatccgga tgcgtgcaca cacgggagga ttccccgatg agtggtgacc agatcccgta
    17821 aatctgtacg ctctgttcgc cgtaatcgtt cagctcatcg gcgaggtcgt aggcggtgcg
    17881 aatcaggtag tctttgcgtg gaacaagtac gccgccctgt ttctctatgt aggtggcaaa
    17941 acatccggca tcagcggcgg caagaaccgc atccattgcg tcatccttca gccgttgcgg
    18001 gccttccggg ttgcgtgcca tctggctggc aaggcggcgc agttcacgcc atacctggcg
    18061 ggaggggatg ccaaagaact ggaactggcg gacccggtga aggcgcgccc agccgatggc
    18121 gcgttccacg ctctcggcca ttgattttcc ggtttcgtgg tcaacgcgtg gcttgcccgt
    18181 tttcgggtcg atgccatcca cggcgcggct gtccaggttt ttcccgatgt aggtggcgat
    18241 gtagctggtt ggcgtgcctt ttgagccgtc tacatactcc gccttaaagc gcggagttat
    18301 gtcatcgccc agctcgtgac gatcttcctg aatggcaata tcgcgggtgt gggacacaat
    18361 ggtgtcgatt tcttccggat gagcaaagac catcatatgc cagtgcacgg tgccgtcatg
    18421 gtgaggctcc accgtgcgga tgccatacca gcgcaggccg tcgcggttca gtttcttgcg
    18481 gaccgccgca aaaaacgtgt taaccaggta atcgctggag tcgcgcatgg tggccccgtt
    18541 ccatttgggg ttcggatgac cgttctccgt tgttgcgtgg tattttgacg ggcaggtgac
    18601 ggtcagaaac accgctctgt cgccacgggc ttcggccaga agttccagtc ccttcatggt
    18661 ggccatcatt tctgctttac ggtgaaccgg gttacttact cccgcgtaat acaccgtctc
    18721 gagatcaatc gtgaacccgt cttcattttc cagcatgaaa cttttcagga aatcgcgtgt
    18781 tttctcgcgc tgtgcgcgaa actcgcttaa cgcgtcctgg ctcagatagg gtgatgtttt
    18841 tctggaaacc agacaggcgg cgcggagttg ttcttctctc cactcgcaac gtaacagcca
    18901 cagtttgcgt ttccaccatt ccgcacaggt caggcgaagg attgcgcccg gcagcagctc
    18961 cgtgtccggt tcgttcctcc ggtctttgtc tgttgtcagt gcgtcataat gtggaggcat
    19021 ggcgtgcagg tgtaacgcca tgcgggccag catctgatac gccttcaggg ttacatccat
    19081 ggtcagctcg ccatcggtcg cgccaaagcc atcgcagagt ttttcgaagg tgctgctgaa
    19141 catcgccgcc gtcatggtgg ccagcgtctg tatctggtgt ttgttgagct gcggcaggta
    19201 aagcaaatcg tccaggcgtt cgcgtccggc aagggagcga taacccggtg tcagccagcg
    19261 tccgtcagtg ctatccagac gttcgaatat tttgcgcagg gttccgcgtg catagcgttc
    19321 cgcctgccag ctctttttgc ctttccggcg atcggcttcc tgttttttgc gcaggaagga
    19381 gaggtggcga ataagcggat cgcgcagata ggacggcagc aggcgcagcg aggccatggc
    19441 ttcatccacc gcgccgcgtg cctgttttct ggcgtctcct gccagtgtga tggttttgtc
    19501 ctgtttttcc tgtgcgtcca ggcttttatt aatcaggttg cccagtggcg tggcggagaa
    19561 cgccgcatca gccatttcct ggcggcgctc gttctctgcc cggtgggcat ccagccagga
    19621 ggaaagcgcg gattcaggag cggggatccc cgttccttca cgccccactg cgtggcgcgg
    19681 ttgttgccag tccctgatgt actctgccgt catagtgatt tacttcgtca tgccattcag
    19741 ggtgtcgcgg cagactgtag ccagccgctg aatttccagc acggtgtctt ctgtgtcggc
    19801 atggcgatgt gtgatgcgga tgctgtcggc aatcacatcg acgattgcag aggatgggcg
    19861 ctggtaaatg ccaataacgg acggggtgcc accttcaatg cggtaaagcc tgtaatttcc
    19921 ctcgtggctg tcaatcatgt agcgaccatc aataacaatc tttccgtcag cgagctgcgg
    19981 tacaggcagg gatttcaggt acatgtcata acgatcacgc acgcgagcgg caagatcacg
    20041 ctctgtgttg agcaggtatt caagaaagtc gttggcgaga atcattgcgg caatcctctt
    20101 gttacagatg tgcgaaggcc tcccgccgca aggtgcagga aaggcccgga acaggaatta
    20161 atggagtttg ttttgctgcc ggatgagctg ctgaagcccg acgtggtttc cggcagttgg
    20221 aggtgctcat gctctgattt ccctcagtag ctggttgaac atctgggtta gtgggttgct
    20281 acacccaaac ggcatcaggt ttacctgata agaaaagcga ccgcctgttt tgcgctcttt
    20341 tcttgtgact aaaccgctgc gccagagacg gcgtagctcc gcattaatgg ttgtggttgg
    20401 tgtattcagt gctgcggcga tttctccact gctacaaccc ggattggcag cgatgtagtc
    20461 cagaatggtc atctgcgtga ctcctgtacc tgtctgataa gattcacctg caccacgttg
    20521 gtggcgcaga agtaagtgcc gtcagtgaga tagatgtgat gtgcatcctt ttctgaacgg
    20581 tgtttgtcga ttgtggtaat caggcgttcg tcaacttcgt attcacgtcc tctggaggtg
    20641 aaacgaacga caggaaaatg cttaattgcc attacgcctc cttggcgtgt gcgaatacct
    20701 ccgcgaatgc ggattgtttt cacattttct tatttaacct ggggtcttat ttgcgcggtt
    20761 attcttcagt gaaaaagcgt tcaatctttt ttactgaatt aataattcgc ataatcccaa
    20821 tggcgcagac caccgaaata atcagaacaa gccatgaaat aaatatactc atgcgatatt
    20881 tcccagctta tacggttcaa tatgttcccc gcattctgcg gcacagatca gctcggaaag
    20941 ttcgttaagt gcatccagat catcagcgta aaaagccacg tcatacagac ttcggattgc
    21001 tctggtcaat gagtcacggg ccgcacgttc agcatgagcg cctgatgcac ttaagcgaaa
    21061 ataaaaacgc tcaagtgctt tgttaatgag agttttatat tctttgccca tcacaacgcc
    21121 ctttaatctg ctttctgtat ttcagcttct gaatccatac aaataaattc gatatagggt
    21181 tcatcgccat taacctggcg tgccttttca gcttcgctaa tgatttctcg tacggtctgg
    21241 tacggaagtt ccacaagcag tcgcgtgccg ttcagataaa cgtaagtggc ttcgtcggct
    21301 ccgtttttac ccgccggagt cactccgtca atagcggatg cacgtaataa cagttcaccg
    21361 cgaaaatcaa taaaacggat aaatacacct tgtgcatggt ctttggtcat aaagcacctg
    21421 ttataaatca gcctgtttaa taaaactttg cccgcgaagc agacgatcaa ccgtgcgaag
    21481 tgcttcgtat aatgtgaaat cctgcccgaa ctgattgtcg ccacagctca atgcaaaaat
    21541 gcggtttccg gtaaacggat tgcgtgggca tttgtggatc acgattccag ctttctcaat
    21601 cagccaggcg tgctcgccga tttgttttac tgggtagcca tccggcgttg cgtgtgtatc
    21661 actcaggctg tagcggatgt tgctgcgtga tgcactggta gtgaaacggt tagcgtggcg
    21721 ttctgttccg gtacaaaaat tacggcgttg cttcagcata aaatgacacc tcgttatttt
    21781 gtcatctgca cgtatttctc tgcgtttctg atggttttca ggaaaattgc gaagagattt
    21841 actttgcgtt ttgtgtttct tccttcttga gtaacgggaa ttaccgatct atcagcctgc
    21901 cttcttacag cgagaatgct ttgatttgtg cgtttcgcat aatcctctag gctttcttca
    21961 agtaccggta gcccatgttc attacggtat gggtagaacg ccgccaaacg ctcaaaatcc
    22021 gcttgttcgt atgtgttaag gacttttgcc atggtgtgat aacctattca atctggtgct
    22081 atttgtggct ctttgtagcg tcaagtggta ctcaactgat aaccaatata gtattcaggt
    22141 gcacaccatg tcaatagaga tatcaaagaa gctaaaagca attcgagaat ctgaggggct
    22201 tagtcaggca aagttcgcgg attcaatagg tattgcggtt ggtacggtta agcaatacga
    22261 gactggtatt cgaggtgtgg gaacggaggt tttactgaaa atcacaatgc acccggaatt
    22321 taaaaaatac actacgtggt tgatgagtaa cgaaacaaat gaggctgctg ggcagatcag
    22381 tccttctctc tcccctgatg ggccagaaaa cacatcgtct tctcaaaaat cccgcaagac
    22441 tggcacacag cccggctaat catggaacgc tgggggcatg gtggtcttgt aacgctgggg
    22501 cttcacgaat gagcataaaa tcaattccgg gagggtatct tcttgacatg cgtcctgagg
    22561 ggcgtaaagg caaacgcatt cgcaaaaaat ttaaaacgaa atcggatgca gttttatatg
    22621 agcggtgggt gctggcgcaa cagcataaca atgagtggaa aggaaactcc attgatcgcc
    22681 gtccgctgtc agtgcttatt gacttgtggt ggaaatacca cggtcagcta atgaagtcag
    22741 ggcataacac gcgccttaaa ttgctgcgct tgagtgaggc aatggatgac ccgtgcgtgc
    22801 ataaacttaa tacaacgatg ctcaccgagc tacgtgtgtc caggatagag caggggatac
    22861 agcccagcac cataaatcga gagattgggg cgttaagcgc gatgtttacc gcactcatct
    22921 catccggcca ttttcttaac gataaccccg ttcaaggcct taaaggaatg aaggttaacg
    22981 agcgcgaaat gggatacctg agtaagtctg aatgtgttca gttgctggat gcactggctg
    23041 aaaatcccga tgaacggctg gctgtcgaaa tccttctgtc gaccggggcg cgatggggcg
    23101 aggtagcggc actggagcag cgccgtgttc ttcattgtcg aatcactttt tcaaaaacga
    23161 agaacagcaa aaaccgtacc gttcctattt ctgaaagcct gtttgaaaag atcaaaaaac
    23221 ggggcgggaa actggtgttt ccgacgctgg attatccatt ggttcgcgat gtcatcaaaa
    23281 cggtcgcacc tgatgttcct gacggccagg ctgttcatgc gctgcgccac accttcgcca
    23341 gtcatttcat gatgaacggc ggcaatattc tgacgctcca gaaaattctg gggcacgcaa
    23401 agattcagac aacgatgatt tatgcccatc ttgcgccgga ttacttgcag gatgcggtga
    23461 gatttaatcc tattgctggg taaggaggat gttgtggaaa aaacagttaa tagcgatttg
    23521 catttaggtc agtactggag aatagacatt gtatgcgatc cagaacttcg agatgaggtt
    23581 gagcaatatt tttctctaca cgatgttggt tttagcaaaa ttgaagtttt ttccgtcgaa
    23641 aatccatata aacttgctct gttctttgat ttcgcgaaga agggtgtgga ggttgcaaaa
    23701 gccattatgg gactcttaga ccgaaatgat attgaaataa cgatgtatcg tgctacggac
    23761 agtagcccgc agtctgtaaa aagcatcaaa ttgcgtaagt ctgaagacgt agagaaatgt
    23821 aaagatttgc ttagtacgtg tgtcgcaatt ggcgttcaac gaaataagga aaaagagggg
    23881 agcgaatgat ccacaaagtg accacatctc tgttatttgt tgtggttggc tgtgtttttg
    23941 tgtgtctgta agtctttgat aattatctaa cttattgatt tttgtttgtg tttatggccg
    24001 ctctgcggcc ttttttcttt tcactgtcga agagtcaccg taaaatcaac gccatgacac
    24061 ttcagcagaa cggataccat gctgacactt gcccgccaac aacagcgaca aaatattcgc
    24121 tggttattat gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt
    24181 gaacaatgga tctcgccagg tgactggttt actcctcgtg gcgaactgtt cgtctggcaa
    24241 attcgcctgc cacgtacgct ggctgtattg ctggttggtg cggcgctggc tatatccggc
    24301 gctgtaatgc aggcgttgtt tgaaaatcct ctggcagaac ctggactact tggcgtctct
    24361 aacggcgcag gcgtggggct tatcgccgcg gtattgcttg ggcaagggca actccccaac
    24421 tgggcgctag ggctgtgtgc gattgctggc gcgcttatca tcactttaat actcttacgt
    24481 ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc attagggatt
    24541 atctgtagcg cactaatgac gtgggctatc tacttttcca cctctgttga tttacgtcag
    24601 ctgatgtact ggatgatggg cggttttggc ggcgtagact ggcggcaaag ctggctgatg
    24661 ctggcattga tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat gaatatgtta
    24721 gcacttggcg agatctcggc gcggcaactg ggtttacccc tgtggttctg gcgcaatgtg
    24781 ctggtggccg cgaccggctg gatggttggc gtcagtgtgg cgctggcggg tgctatcggc
    24841 tttattggtc tggtgatccc acatattctt cggttgtgtg gtttaaccga tcatcgcgta
    24901 ttacttcccg gctgcgcgct ggcaggggcg agcgcattgc tgctggccga tattgtagcg
    24961 cgcctggcat tagctgccgc agagctgcct attggcgtgg tcaccgcaac gttgggtgcg
    25021 ccggtgttta tctggttatt gttaaaagca agacgttagc cgcaaaaaga cggtctatga
    25081 ttaaaagcta agatttttac tgaccacacc caggagaaac gatgcaagat tccattctga
    25141 cgaccgtagt gaaagatatc gacggtgaag tgaccacgct ggagaagtac gctggtaatg
    25201 tgctgttgat tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat gagcagttgg
    25261 agaatattca gaaagcctgg gccgatcgag gttttgtggt gctgggattc ccgtgcaacc
    25321 agtttctgga acaagaaccg ggcagcgatg aagagattaa aacttactgt accaccacat
    25381 ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa tggcgaagga cgccatccgc
    25441 tgtatcaaaa attgattgcc gcagcgccga ccgcagtcgc gccggaagag agcggattct
    25501 atgcccgtat ggtcagcaaa ggtcgtgcac cgctgtaccc tgatgatatt ttatggaatt
    25561 ttgagaaatt tttagttggc agggacgggt tggtcatcca gcgtttttcc ccggatatga
    25621 cgcctgaaga tcccattgtg atggaaagca ttaaactggc gttggcaaaa taatgtctat
    25681 tgtgatgcag ttacaggatg ttgcggaaac tacccgcctg gggccgcttt ctggcgaggt
    25741 tcgggctggg gagatcctgc acctggtggg gccgaatggc gcgggtaaga gtaccttgct
    25801 tgcacgaatg gccggaatga ccagcggtaa gggaagcatt cagttcgcgg ggcaaccact
    25861 ggaagcatgg tccgcaacaa aactcgcgct gcatcgcgcc tacctttcac aacagcagac
    25921 gccgccgttt gcaatgccgg tctggcacta cctgacactg catcagcacg ataaaacgcg
    25981 taccgaacta ctgaatgatg tcgcaggggc gctggctctt gatgacaaac tcggacgtag
    26041 caccaatcaa ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg tggtgctgca
    26101 aatcacacca caagccaatc ccgcaggtca attgctgctt cttgatgagc cgatgaacag
    26161 tcttgatgtt gcgcaacaaa gtgcgttaga caaaattctg agcgctctgt gtcagcaagg
    26221 actggcgatt gtgatgagca gtcacgatct caaccacaca ttgcgtcatg cgcatcgggc
    26281 gtggttgcta aaaggtggaa aaatgctggc cagtggacgc agagaagagg tgctcacgcc
    26341 gccaaatctg gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg aaggtcacag
    26401 aatgctgatt tcgaccatct gatttcatta gtatcttgaa aatcgggtaa ttacgacgct
    26461 aaattaatgc aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg ccttattttg
    26521 atcacagcac tgtttctggc cgggtgtagc caccataaag caccgccgcc aaatgccaga
    26581 ctttctgatt cgattaccgt tattgccggt ttgaacgacc agctacaaag ctggcatggc
    26641 acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg actgttcggg atttgtggtt
    26701 gtaacgatgc gcgatcgttt cgatttgcag ctgccccgag aaaccaaaga acaagcctct
    26761 atcggcacgc aaattgataa agacgagttg ctgcctggtg acctggtctt tttcaaaacg
    26821 ggttccggac aaaatggttt gcatgtaggt atttacgata ccaacaacca atttatccac
    26881 gcctctacca gcaagggagt gatgcgttcc tcacttgata atgtctattg gcagaaaaat
    26941 ttctggcagg cgagacgaat ttagtatgtc gaaaaatgga cggcgatgat acatcgccgt
    27001 ccatttactc ctgaatacag tagtattttg cagacaaaaa ttccgtaaat ggattattcg
    27061 tttataatca ggataatcta aaaaaaggac ccctgaaaaa gtggttaaca tggaacaaat
    27121 gaaagttact ccttatgtaa ttgttatgtc ttatggcctc atccctttta gccggatact
    27181 gaaaaacatc cttcgagagg gacggttacc atgaagattt ttttggagaa tctttatcat
    27241 tctgattgct atttcctgcc gataagagat aaccagcaag accttgtcgg cgttgagtta
    27301 attacccatt tctccagtga agatggtacg gttcgtatcc cgacgagtcg ggttatagcg
    27361 caacttaccg aggagcaaca ttggcaatta ttttcagagc agttggaatt actaaaatca
    27421 tgccaacatt tttttattca acataaactc tttgcctggc ttaatttaac gccacaagtg
    27481 gctacattat tattagagag ggataattac gcaggcgaac tattaaaata tccgtttatt
    27541 gaactgttga ttaatgagaa ttacccgcat ctcaatgaag gaaaagataa tcgcgatctt
    27601 ttatccttat cgcaaatgta tccccttgtg ttaggcaatt tgggagcagg taatagcaca
    27661 atgaaagcgg tttttgatgg tcttttcacc cgcgtcatgc tggataaaag ttttattcag
    27721 caacaaatca cgcatcgctc cttcgaaccg tttattcgtg cgatccaggc gcaaatttcc
    27781 ccttgttgca attgcatcat cgcggggggc attgatacga cagaaatatt ggcacagatt
    27841 acccccttcg atttccatgc tctccaggga tgcctgtggc ctgccgttcc tataaatcag
    27901 ataacgacgt tggttcagcg ataaccctcc tgtttgctgg tgtttaagac gagagtaacc
    27961 gtctacacta ttaaacagga ggatctatga ccctgtcttt tattacccgc tggcgcgatg
    28021 aattgccagc aacctataca gcactttccc ctacgccttt aaataatgcc cggctgattt
    28081 ggcataatac cgaactcgct aacacgctga gtattccatc gtcgctgttt aaaaatggcg
    28141 caggtgtctg gggcggcgaa accctactgc ctggtatgtc accactggcg caggtttaca
    28201 gtggtcatca gttcggcgtc tgggcgggcc aactgggtga tgggcgcggt attttactcg
    28261 gcgaacaact gcttgctgat ggcactacaa tggactggca tctgaaaggt gctggcctga
    28321 cgccttattc gcgaatgggt gatggacggg cggttttacg ttcgacgata cgagaaagtc
    28381 tcgccagtga ggcgatgcat tatctgggca ttccgacgac ccgcgcgtta agtatcgtca
    28441 ccagcgattc gccagtgtat cgggaaacgg tggagccagg cgcgatgctg atgcgtgtgg
    28501 caccaagtca tctgcgcttt ggtcatttcg aacattttta ctatcgccgc gagccggaaa
    28561 aggttcgtca gttagctgac tttgccattc gtcattactg gtcatatctt gaagatgatg
    28621 aggacaaata ccgcctctgg tttagcgatg ttgtcgcacg taccgcatcg ttaattgccc
    28681 aatggcagac ggtcggcttt gctcatgggg tgatgaatac cgataacatg tcgctgctgg
    28741 ggctgacgct tgattacggg ccgtttggtt ttctggatga ttacgaaccc ggttttattt
    28801 gtaatcactc ggatcatcaa gggcgttata gctttgataa tcaacctgct gtcgcgttgt
    28861 ggaatttaca gcgtctggcg cagacattgt caccatttgt tgccgtagat gccctgaatg
    28921 aggccctgga cagctatcag caggttttgt tgacgcatta tggacaacgg atgcggcaga
    28981 aactgggctt catgacggag caaaaagagg ataacgcgct actgaatgaa ttattcagtc
    29041 tgatggcgcg agagcgcagc gattataccc gcacattccg catgctgagt ctgaccgagc
    29101 agcacagcgc ggcgtcaccg ctacgtgatg agtttattga tcgtgcggca tttgatgact
    29161 ggtttgcccg ttatcggggg cgtttgcaac aagacgaggt tagcgatagt gagcgtcagc
    29221 agctgatgca aagcgttaac ccagctctgg tgttgcgcaa ctggttggcg caacgggcga
    29281 ttgaagcggc agaaaagggt gatatgacgg aattgcaccg cctgcatgag gcgttgcgaa
    29341 atcctttcag cgacagagct gatgactatg tcagtcgtcc acctgactgg ggtaaacggc
    29401 tggaagtcag ctgctcgagt taaaggctac ttggtcaaca gcagcttgcc agcctgagtt
    29461 ttacggagca gatattcttg cccgtcatga tcgataatca gtttgccatc cggacctaac
    29521 agtgtctggc tggaaattcg ccgaataggc tgcggggaag cggtcttgtg attggcatcc
    29581 gtttcaggcg tgagttttct gctatccgta taacgcataa aaattaataa taattgatat
    29641 aacaatgatt cttattatca atattatggc tgaccagagc aagcagtaat gtgctgagaa
    29701 gacaacaagt tgcgcagaaa tgcccatccc atcaggaatg ggcacgcatt cagaagcggg
    29761 tatctaccgc agaggcaagt ttttcgacca ggcgttcggt atcttcccag cccagacacg
    29821 ggtcggtaat ggattgaccg taagtgagcg gctgaccgcc gacgattttt tgcgttcctt
    29881 cgcgcaggaa actttccgcc ataattccag caatcgccgt agagccattg cggatttgct
    29941 gacaaatatc ctcacaaact tctaactggc gacggtgctg cttctggcag ttaccgtggc
    30001 tgaaatccac caccagatgt tcaggtaaat caaactcgtg cagcgtatcg caggctgcgg
    30061 cgatatcatc ggcatgataa ttcggttttt tgccgccacg cataataatg tggccatacg
    30121 ggttgccgct ggtctgatag atggtcatct gaccattttt gtctggcgag agaaacatat
    30181 ggctggcgcg ggctgcgcgg atagcatcca cggcaatccg cgagttgcca tcggtaccat
    30241 ttttaaaacc taccggacag gagagtgccg aagccatttc acggtggatc tgactttcgg
    30301 tagtacgtgc gccaatcgcg ccccaactga ttaaatcagc aataaactga ccggtcacca
    30361 tatcgaggaa ctcagtcgcg gtagggacac ccagctcatt tacctgtaaa agtaatttgc
    30421 gcgccatctc cagaccgtga tttacccgat agctgccgtt taaatccgga tcggagatta
    30481 gccctttcca gccgacaaca gttcgtggtt tttcaaaata ggtgcgcatt acgatttcca
    30541 gccgtgactg gtactggttg cgcagcgact gcagacgggt ggcgtactcc attgcagcgg
    30601 tgagatcgtg gatcgagcag gggccaataa tgaccaacag tcgcttatct tcaccattca
    30661 gtattttttc aattctgcgg cgggagtcgg tgacatgggt ggcgacgcca ggcgttacgg
    30721 gataccgtag cgcgagttcg gcgggcgtta ccaggctctc aatacgcgca gtacggagtt
    30781 cgtcagttct gttcattaca agtctcagaa atttgttgct tctacggcag gactaccggg
    30841 aagtgatgcg catcacgata aaccaatcgc agctgatttc aagtgcgagg tgtgtcacac
    30901 ctcgccgggt ggttagcatg ataacaaaaa aataagctaa tgcactagtt ctctagtaca
    30961 ttcggcggct aagccccatg atatcgagga gcttggtggc aatctcttct accgaataat
    31021 tggtactgtt aatccacggg atctgatttt tacggtacaa cgcttctact tccgcgactt
    31081 ccatcctgca ctgacgaagc gaggcatagc ggctgttctc ccgacgttcc tcgcgaatcg
    31141 ccgccagacg ttccgggtcg atagtcaggc cgaacaattt atgctgaagc ggtttgagcg
    31201 acgcgggtag caccagattg tccatatcgt cggcaataaa ggggtagttt gccgcgcgga
    31261 taccaaactg cattgccaga tacagactgg tgggggtttt accacagcga gaaacaccga
    31321 gcaggatcac ctgagcctgg tccagattgc gcaacgaaat gccgtcatcg tgggcgaggg
    31381 tgtaatcaat cgccgcaatg cgcgcatcat atttattgag attattaggg ttaaggccat
    31441 gggtacgatg agcaatcggc gttggatcca gtttcatctc ttgttgtagc ggggcaacca
    31501 gcgcctgaac aatatcctgg caaaagcctt cactttgcaa gatgatggcg cgaatctccg
    31561 gcaacacgat ggagtagaag accagcgggc gcacacctgt ctggtgataa attgcgtcaa
    31621 tctgatcctt cactgcccgt gcacggctct cattttcgac aaacggcagc gtaatgctgc
    31681 tgatagtgac gggaaattgt gacattactg cgtgtcctaa tacctccgca gttattgccg
    31741 taccatcaga aatataaaaa acgtggcgat caacagcatt atccattttg ttcttcccgt
    31801 gatgcagaca taattctatg aaagcataaa ttaaaaacgc acagaagcgt aggacgtaat
    31861 gtctggttta taaaatgaac cttcaatttt attttttatg aaaacagcat ttcattttta
    31921 tggtttcgtt tataccgatg gtttatgtgg aaattgtcga atagagcagg tttgcgcaac
    31981 gctgggatca gtcttaaaaa gtaaaaaaat atatttgctt gaacgattca ccgttttttt
    32041 catccggtta aatatgcaaa gataaatgcg cagaaatgtg tttctcaaac cgttcattta
    32101 tcacaaaagg attgttcgat gtccaacaat ggctcgtcac cgctggtgct ttggtataac
    32161 caactcggca tgaatgatgt agacagggtt gggggcaaaa atgcctccct gggtgaaatg
    32221 attactaacc tttccggaat gggtgtttcc gttccgaatg gtttcgccac aaccgccgac
    32281 gcgtttaacc agtttctgga ccaaagcggc gtaaaccagc gcatttatga actgctggat
    32341 aaaacggata ttgacgatgt tacccagctt gcgaaagcgg gcgcgcaaat ccgccagtgg
    32401 attatcgaca ctcccttcca gcctgagctg gaaaatgcca tccgcgaagc ttatgcacag
    32461 ctttccgccg atgacgaaaa cgcctctttt gcggtgcgct cctccgctac tgcagaagat
    32521 atgccggacg cttcctttgc cggtcagcag gaaaccttcc tcaacgttca gggttttgac
    32581 gccgttctcg tggcagtgaa acatgtattt gcttctctgt ttaacgatcg cgccatctct
    32641 tatcgtgtgc accagggtta cgatcaccgt ggcgtggcgc tctccgccgg tgttcaacgg
    32701 atggtgcgct ctgacctcgc atcttctggc gtgatgttct ccattgatac cgaatccggc
    32761 tttgaccagg tggtgtttat cacttccgca tggggccttg gtgaaatggt cgtgcagggc
    32821 gcggttaacc cggatgagtt ttacgtccat aaaccgacac ttgcagcgaa tcgcccggct
    32881 atcgtgcgtc gcaccatggg gtcgaaaaaa atccgcatgg tttacgcgcc gacccaggag
    32941 cacggcaagc aggttaaaat cgaagacgta ccgcaggaac agcgtgacat cttctcgctg
    33001 accaacgaag aagtgcagga actggcaaaa caggccgtac aaattgagaa acactacggt
    33061 cgcccgatgg atattgagtg ggcgaaagat ggccacaccg gcaaactgtt cattgtgcag
    33121 gcgcgtccgg aaaccgtgcg ctcacgcggt caggtcatgg agcgttatac gctgcattca
    33181 cagggtaaga ttatcgccga aggccgtgct atcggtcatc gcatcggcgc gggtccggtg
    33241 aaagtcatcc atgacatcag cgaaatgaac cgcatcgaac ctggcgacgt gctggtcact
    33301 gacatgaccg acccggactg ggaaccgatc atgaagaaag catctgccat cgtcaccaac
    33361 cgtggcggtc gtacctgtca cgcggcgatc atcgctcgtg aactgggcat tccggcggta
    33421 gtgggctgtg gtgatgcaac agaacggatg aaagacggtg agaacgtcac tgtttcttgt
    33481 gccgaaggtg ataccggtta cgtctatgcg gagttgctgg aatttagcgt gaaaagctcc
    33541 agcgtagaaa cgatgccgga tctgccgttg aaagtgatga tgaacgtcgg taacccggac
    33601 cgtgctttcg acttcgcctg cctgccgaac gaaggcgtgg gccttgcgcg tctggaattt
    33661 atcatcaacc gtatgattgg cgtccaccca cgcgcactgc ttgagtttga cgatcaggaa
    33721 ccgcagttgc aaaacgaaat ccgcgagatg atgaaaggtt ttgattctcc gcgtgaattt
    33781 tacgttggtc gtctgactga agggatcgcg acgctgggtg ccgcgtttta tccgaagcgc
    33841 gtcattgtcc gtctctctga ttttaaatcg aacgaatatg ccaacctggt cggtggtgag
    33901 cgttacgagc cagatgaaga gaacccgatg ctcggcttcc gtggcgcggg ccgctatgtt
    33961 tccgacagct tccgcgactg cttcgcgctg gagtgtgaag cagtgaaacg agtgcgcaac
    34021 gacatggggc tgaccaacgt tgagatcatg atcccgttcg tgcgtaccgt agatcaggcg
    34081 aaagcggtgg ttgaagaact ggcgcgtcag gggctgaaac gtggcgagaa cgggctgaaa
    34141 atcatcatga tgtgtgaaat tccgtccaac gccttgctgg cagagcagtt cctcgaatat
    34201 ttcgacggct tctcaattgg ctcaaacgat atgacacagc tggcgctcgg tctggatcgt
    34261 gactccggcg tggtgtctga attgttcgat gagcgcaacg atgcggtgaa agcattgctg
    34321 tcgatggcta tccgtgccgc gaagaaacag ggcaaatatg tcgggatttg cggtcagggt
    34381 ccgtccgacc atgaagactt tgccgcatgg ttgatggaag aggggatcga tagcctgtct
    34441 ctgaacccgg acaccgtggt gcaaacctgg ttaagcctgg ctgaactgaa gaaataaaat
    34501 aaatccccgg cggcgttcag tcgccgggtt tatgtgatcc ccgaagataa aacttattca
    34561 atctcttcac agacatcctg cgttaatcgc cgcataatat cttttcttaa caaaaacttt
    34621 tgtattttac ctgaggcagt gcgcggtagt ttttcgatta ccacgatatg ttcaggatat
    34681 ttatattttg cgacccgttt acggctaaaa aaagccacca cctcttccag cgataatgaa
    34741 tgatgcggcg ctttcagcac gacataagcg catgatcgtt cacctaaacg ttcatcgggc
    34801 attgcaacca cacaggcatc gtgaatttta ggatgctgca ataaaatatc ttccacttca
    34861 cggctgctaa tattttcgcc gccgcggaca ataatatctt ttttgcgtcc ggtaattttt
    34921 atatagccag cctcatccat acggcagaga tcgccgctgt aataccagcc ttcttcatcc
    34981 agcgcacggg cggttaattc aggttcatca aaatatccca taaacacatt ggggccacgc
    35041 gaggcttctt caccttcgca acctggcggt aaggtcttgc gtgcgtcatc gaccacttta
    35101 atctctacac ctgcggcagc gtaaccatcg gtgtgcataa agcgcgacaa aggatcatcg
    35161 agattcacca ccgcatgcgg cgaactttct gtggaaccat aaacacttaa taatttaatg
    35221 ccgagctgct ggcattcacg cgccactttt ttggggattg tggttccgcc gcaaagaaag
    35281 aaacgcagcg ctgacaggtc cgcgggttgt ttctctagta cattcaaaag atcatagaca
    35341 aacggcgttg cgcctagcat acaggtgcaa cgctgctgct caagcagcgc gagacacgca
    35401 tcaggagtga aaatatctaa caacacgctg cgagcgccaa ttaagaatgg tgccgttacg
    35461 ccatgcagaa agcccgttgc gtggccgagt ggcgcaggca tcataaagac atcctgccag
    35521 gtcagattca gccgcgcgca ataagcccgc tcactggcga gaatattgtt atgcgttagc
    35581 atcacgccct ttggcagacc ctcggttccg gaggtaaaca gcaccgcagc taattcatcg
    35641 ccgtgggtcg ttatcgccgt ggtcagtggg gtattgtcgg cgataatctg acttaatgag
    35701 agggaagagg tggcgggagc cagtttgtcc acgccgacaa tttgttgtag ttgtggaagc
    35761 tgattttgca gcggcaggat taaatctacc gggcgcgttt gtttaaacaa cgtcggtgca
    35821 aagaacattt ttgcctgaca cttattgagc acccacacca gttctgcttc ccgccaggaa
    35881 ggcaacagcg gcacggaaac cgcaccgatt ttcaggcagg caagatagat aacggtaaat
    35941 tcacaccagc caggcagttg aaatgcgatg cgatcgcctg attcaatacc cttcgctaac
    36001 atccagtttg ccagacagct cgcggcgtga tcgagcgcgc tataggtgta cgatgcacca
    36061 tgattatcga ccacggcaat tttgtctggc atcgcacgag cggtctgctg ccagtaatcg
    36121 gccagcgaag catcgcccca tagcccttgc tgacgatacg ccgcacgacg ttgttcgttg
    36181 aacgttaatg tcactttcat gttggactct cgaaacagaa catcaggccc gagatgcggg
    36241 cctgtgggat gcatgattag ccgtagcgaa aatcaatacc gaaggtgcct gcgggatatt
    36301 gccactgttc gagaatagtg ttaccgcaca gcacccgaca ggtgccgcac tccagacaac
    36361 cggcagaatc aaaatgaatg ttgccggcgt catcctgctt ataaagtccg gcagggcagg
    36421 ctttcattaa tttacggaat tcattgatat cgggatttgc cgccagaatg atatgcgggt
    36481 ggccctcatc aacatggaat ttattgacgc ctaatttgat gtcgacgtta accgtagcgt
    36541 tctggctcat agcgcggttg ctcccttaat gccatctttc agcaagttga tcagcccaat
    36601 tttcttcgca tgtcccatga tcattttgcg taccggctgg tttggtttgc cgtcaatggt
    36661 gaacatctcg ttcatgatgt cggcgaccat tcgtgggtat tggctaaaca ggcgcgggtt
    36721 ttccatcagc gccgggatct tgcggaaatg ctgcatatcg cgcatgatgc agctttgttc
    36781 cagctcacgt ttgtattgcg ccagactgct ggcggagaaa tcctcgcgtt ctttggcggc
    36841 gatcaccgtt gtggcggcag cctgagccga tgcaatggct aaatccatgc cacgaactgt
    36901 aaaacccaaa ttcaggcaga agcctgcggc gtcaccaacg atcatcacgc catcgttaac
    36961 catctgcggc accattgcca gaccgccttc tggcaccata tgcgcggaat attcaagcag
    37021 tttgccgccg ctaattagtg ggcgaatggc ggggtgttgt ttaaaatctt ccagcatttg
    37081 cggcacgctt ttttgcgcat gggcgatatc acccaatcca caaaccagcc ccaaggatat
    37141 ggaatccttg ttggtataga gaaatccccc gcccatcagg ccgtcagaag gggaaccggc
    37201 aaacagccag gcggcacctt cctcgcccgt aatattaaag cgatcgttga tctgttctgg
    37261 tgtgaggcca ataacctctt taacaccaac agcgtaatga tgcggatcgg aagcgggaac
    37321 cattcccagc gagcggccaa gcatcgagtt aacgccatca gccagaatca ccacattcgc
    37381 ttcgagaata tcatccccag cctgcacgcc agtgaccttg tttccttcac gaaccagcgc
    37441 atcgacgcga acgcctggga taaactgcgc gcccgcctgc tcggcttgtt ccatcaacca
    37501 cgggtccaga cgattacgca atacggtata agatgcgtgt tgcggaacat ctggttgctc
    37561 gcggtgaaaa tcgagggtaa cggcgctttc ttcggttaag aaggagattt tctcgcgtgt
    37621 gaccttgcgt tctaccggcg ctgatactgc aaagcctgga atgattgctt caagtgtgtg
    37681 ggcataaaga cgcccgccgg tcatgttttt acatccggca ctgtcgccgc gttctatcac
    37741 caggacatcc agcccggctc gcgccatgac cagtgcggca acgctaccag caacgcccgc
    37801 accgaccaca atggcatcaa atttgtcttc cgacatgcgt tatgctccct tcccgctgcg
    37861 tttacagcgg gagatgtggt tgaaagggga attagcggct caactggcta atcagggcag
    37921 ggacgacttt gtagatatcg cccaccagac cgtagtcggc ataattgaag attggcgcat
    37981 ttttatcttt attgatggcg acaatcactt ttgcgccgtt gccgccaacc atatgctgga
    38041 tctgcccgga gatccccagc gtcaggtaga gatcggattt cagcaacacg ccggagacac
    38101 cgatataacg ttcacgctcc atccagttct cgccttcggc aattggacgt gaacagccga
    38161 cttcagcatt cagcaccgcc gccagttcgt ggaccatttt caggtcatcc tgcgccgcca
    38221 gaccacgacc gacgccaacc acacgttttg ctttgctcag gtccacgctg cttgcggctt
    38281 tcgcacggcg ttcgcgacag agaatttcat gacgcggagc aacataaggt accgtttctg
    38341 tcgggcactg atgagaggta tcactggtgc acggttcctg aacaccgggt gcaagggtaa
    38401 tgatcgccag cgggctgttg atcttttcct gagcgaacgc taacccgcca tacatccggt
    38461 gttcggcgca aatgtgacca tcgacaatat ccaccgccgt ggcatcgttc accagcgccg
    38521 cattcagttg cacacttaac cgtgctgcca gagctttacc acgtttcgtc gcggccaaca
    38581 gcaacatagc ggggtgttta tctttcagca gggcagcaat gctttcggcg taattttcag
    38641 tgcgttgcag cgcgtcgttt tgctcaagaa cataaataca ttttggacca taaggcataa
    38701 ctgcctgcgc ctggtcggta ttttgtacaa tggcatacac ctgttggccc cattgctgag
    38761 cgccgccaaa cagttcagca taacgttcag gattatcgct aaagacccag acgctgttta
    38821 attgactcat cgtttcttct ccgtaggctt cagttcaggg ctttcttcag atgttccgcc
    38881 agctcggcaa tggcctccgg cgaatcgtta tcgataatga tgtgcttacg ttctgtttgc
    38941 ggcggtacgc gaatgccgac cagttcagca agtggcgcgc tctggctcca gtcaatatca
    39001 cttgcctgcc actgatttac cggtttttta cccgcgccga gaatggcttt catcgaagga
    39061 atgcgtggca cgttaatatc ggaggtgacg cagagcacgg ctggaacaga gagttcaata
    39121 acttcaacat catcttcaag cgtgcgttca atcaccagtg tattgccctg acgctgaata
    39181 gcactcactg cattaatcac cggaagttgc agaatttctc cgaccagcaa gccaacctgc
    39241 tgggcataaa ggtcgccgga accttcacca aagatcagta aatcgaagcc gatcttttca
    39301 attgctgccg ccagcgcttt tgcggtatcg agaggcagtg catgttcaag ttgcgcatcc
    39361 tgcaccaaat acaggctgtg cggcccgcgg gatagcacgt ctttgcgcac tttcgagttc
    39421 tgcaacaatg agccgccaac ggtcagcgcg gctatctcat cgtcatctgt tgcgagctgg
    39481 cttgcagctt caatggcatt gagatcgaac tggctgattt tggcgtcggc attgtcgaaa
    39541 ttcagggtgt attctggagt gacaacaatg tcctgttctt caggcaccag cttaaagcag
    39601 gttattattt tcatgatttc tcctgtgagt cgcatacgga accatctccg atggaaggta
    39661 aaaatggcct gaaaatagtg tgcgatagcg gtgcggggtt ttcgacggag caagctgttt
    39721 tccttgccaa caatcttgca aaaaaaatgc gatcgctaaa acaaaagtgc aatttgcggc
    39781 aatatagtta ttccatgacc gtacaatctt gcattcagtg taaataatct tgtttagctc
    39841 tcgctaaacg cggttgtata accgcaatac acgccgtatt ttatccggca tatattgttt
    39901 ttgcttgctc atggaatagg gaggttgtca tgtatcaacg ctgttttgat aacgccagtg
    39961 aaacgctgtt tgtcgccggt aaaacgccac ggctttcacg ttttgcattt agcgatgatc
    40021 caaaatggga gtccggacat cacgttcatg acaatgaaac cgagttgatt tacgtcaaga
    40081 aaggggttgc aaggtttacc atcgattctt cgttatatgt cgcacatgcg gatgacattg
    40141 tggtgataga acgcggcagg ctacatgcgg tggcctctga cgttaacgat ccggcaacga
    40201 cgtgtacctg tgcgctgtac ggctttcagt ttcagggggc tgaggaaaat cagctactgc
    40261 aaccgcattc ttgtccggta attgccgcgg ggcagggaaa agaagtcata aaaaccttat
    40321 ttaatgagct aagtgtgatt ttgccgcaaa gtaaaaatag ccaaacatct tcgttatggg
    40381 atgcatttgc ctatacgtta gccattcttt actacgaaaa ttttaaaaat gcttatcgtt
    40441 cggagcaggg gtatatt
//
LOCUS       DAZGOB010000035        39592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGOB010000035 DAZGOB010000000
VERSION     DAZGOB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.035"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            166..1020
                     /locus_tag="ACHLIF_001881"
     CDS             166..1020
                     /locus_tag="ACHLIF_001881"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFO9605910.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            1056..1946
                     /locus_tag="ACHLIF_001882"
     CDS             1056..1946
                     /locus_tag="ACHLIF_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO9605911.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            2007..3506
                     /locus_tag="ACHLIF_001883"
     CDS             2007..3506
                     /locus_tag="ACHLIF_001883"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9605912.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            3507..4496
                     /locus_tag="ACHLIF_001884"
     CDS             3507..4496
                     /locus_tag="ACHLIF_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9605913.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRRYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            4518..5480
                     /locus_tag="ACHLIF_001885"
     CDS             4518..5480
                     /locus_tag="ACHLIF_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9605914.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            5477..6523
                     /locus_tag="ACHLIF_001886"
     CDS             5477..6523
                     /locus_tag="ACHLIF_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO9605915.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEMSALKAEIAGYR"
     gene            complement(6615..7964)
                     /gene="accC"
                     /locus_tag="ACHLIF_001887"
     CDS             complement(6615..7964)
                     /gene="accC"
                     /locus_tag="ACHLIF_001887"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO9605916.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7975..8445)
                     /gene="accB"
                     /locus_tag="ACHLIF_001888"
     CDS             complement(7975..8445)
                     /gene="accB"
                     /locus_tag="ACHLIF_001888"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO9605917.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPIMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9311..10141)
                     /locus_tag="ACHLIF_001889"
     CDS             complement(9311..10141)
                     /locus_tag="ACHLIF_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605918.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(10178..11152)
                     /locus_tag="ACHLIF_001890"
     CDS             complement(10178..11152)
                     /locus_tag="ACHLIF_001890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO9605919.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            11304..13244
                     /gene="csrD"
                     /locus_tag="ACHLIF_001891"
     CDS             11304..13244
                     /gene="csrD"
                     /locus_tag="ACHLIF_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO9605920.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13549..14592
                     /gene="mreB"
                     /locus_tag="ACHLIF_001892"
     CDS             13549..14592
                     /gene="mreB"
                     /locus_tag="ACHLIF_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO9605921.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            14658..15761
                     /gene="mreC"
                     /locus_tag="ACHLIF_001893"
     CDS             14658..15761
                     /gene="mreC"
                     /locus_tag="ACHLIF_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO9605922.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            15761..16249
                     /gene="mreD"
                     /locus_tag="ACHLIF_001894"
     CDS             15761..16249
                     /gene="mreD"
                     /locus_tag="ACHLIF_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO9605923.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            16258..16851
                     /gene="yhdE"
                     /locus_tag="ACHLIF_001895"
     CDS             16258..16851
                     /gene="yhdE"
                     /locus_tag="ACHLIF_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO9605924.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            16841..18310
                     /gene="rng"
                     /locus_tag="ACHLIF_001896"
     CDS             16841..18310
                     /gene="rng"
                     /locus_tag="ACHLIF_001896"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO9605925.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            18378..22178
                     /gene="yhdP"
                     /locus_tag="ACHLIF_001897"
     CDS             18378..22178
                     /gene="yhdP"
                     /locus_tag="ACHLIF_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO9605926.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            22334..23779
                     /gene="tldD"
                     /locus_tag="ACHLIF_001898"
     CDS             22334..23779
                     /gene="tldD"
                     /locus_tag="ACHLIF_001898"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO9605927.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNCR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(23907..24836)
                     /gene="aaeR"
                     /locus_tag="ACHLIF_001899"
     CDS             complement(23907..24836)
                     /gene="aaeR"
                     /locus_tag="ACHLIF_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO9605928.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            25019..25222
                     /gene="aaeX"
                     /locus_tag="ACHLIF_001900"
     CDS             25019..25222
                     /gene="aaeX"
                     /locus_tag="ACHLIF_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO9605929.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            25230..26162
                     /gene="aaeA"
                     /locus_tag="ACHLIF_001901"
     CDS             25230..26162
                     /gene="aaeA"
                     /locus_tag="ACHLIF_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO9605930.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            26168..28135
                     /gene="aaeB"
                     /locus_tag="ACHLIF_001902"
     CDS             26168..28135
                     /gene="aaeB"
                     /locus_tag="ACHLIF_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO9605931.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     SMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            28227..28499
                     /locus_tag="ACHLIF_001903"
     CDS             28227..28499
                     /locus_tag="ACHLIF_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO9605932.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(28555..28818)
                     /gene="yhcN"
                     /locus_tag="ACHLIF_001904"
     CDS             complement(28555..28818)
                     /gene="yhcN"
                     /locus_tag="ACHLIF_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO9605933.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(29183..29653)
                     /gene="argR"
                     /locus_tag="ACHLIF_001905"
     CDS             complement(29183..29653)
                     /gene="argR"
                     /locus_tag="ACHLIF_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO9605934.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            30088..31026
                     /gene="mdh"
                     /locus_tag="ACHLIF_001906"
     CDS             30088..31026
                     /gene="mdh"
                     /locus_tag="ACHLIF_001906"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO9605935.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            complement(31089..32156)
                     /gene="degS"
                     /locus_tag="ACHLIF_001907"
     CDS             complement(31089..32156)
                     /gene="degS"
                     /locus_tag="ACHLIF_001907"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO9605936.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(32246..33613)
                     /gene="degQ"
                     /locus_tag="ACHLIF_001908"
     CDS             complement(32246..33613)
                     /gene="degQ"
                     /locus_tag="ACHLIF_001908"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO9605937.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(33767..34165)
                     /gene="zapG"
                     /locus_tag="ACHLIF_001909"
     CDS             complement(33767..34165)
                     /gene="zapG"
                     /locus_tag="ACHLIF_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO9605938.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            34359..35486
                     /gene="zapE"
                     /locus_tag="ACHLIF_001910"
     CDS             34359..35486
                     /gene="zapE"
                     /locus_tag="ACHLIF_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO9605939.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            35705..36133
                     /gene="rplM"
                     /locus_tag="ACHLIF_001911"
     CDS             35705..36133
                     /gene="rplM"
                     /locus_tag="ACHLIF_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO9605940.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            36149..36541
                     /gene="rpsI"
                     /locus_tag="ACHLIF_001912"
     CDS             36149..36541
                     /gene="rpsI"
                     /locus_tag="ACHLIF_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO9605941.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            36936..37574
                     /gene="sspA"
                     /locus_tag="ACHLIF_001913"
     CDS             36936..37574
                     /gene="sspA"
                     /locus_tag="ACHLIF_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO9605942.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            37580..38077
                     /gene="sspB"
                     /locus_tag="ACHLIF_001914"
     CDS             37580..38077
                     /gene="sspB"
                     /locus_tag="ACHLIF_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO9605943.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(38120..39487)
                     /gene="dcuC"
                     /locus_tag="ACHLIF_001915"
     CDS             complement(38120..39487)
                     /gene="dcuC"
                     /locus_tag="ACHLIF_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO9605944.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVAV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9690 a         9528 c        10452 g         9922 t
ORIGIN      
        1 tgtaaagtga aaaacaaatt aagttatttt ttgtaagtta ttttgatgat attcgacatg
       61 catcacgctt tttgccgctt ttgctgtgct agtttgcttt caaaatgaaa gtgaatattc
      121 tctttcttca ttttgtaagg cgaacgacaa ataacaggag ctgcgatgta catcatttca
      181 agcaaaaaca tgctgaaaaa agcccagtca gagggatatg ccgtccccgc tttcaacatt
      241 cataaccttg aaactttaca ggtagttgtt gagagcgctg cacaacttcg ctctcccgtt
      301 atgctggcgg gaacacccgg tacttatcgt tatggtggcg tgggaagttt aatttcgtta
      361 gtacaatcgc tggctcgcga gtacaacctg ccgttagtcc tgcatctcga ccatcatgaa
      421 gagagtgatg acatttttaa caaggttcgc gcagggatcc gttctgtcat gattgatggc
      481 tcccatttgc catttgaaga caacattgcg cgagtgcaag aagttaccac cttttgccat
      541 cgctttgatg ttagcgttga ggccgagcta gggcgtcttg gcggccagga ggacgatctg
      601 ctggtggatg aaaaagattc cgcttatacc tcaccagccg cagctgcaga gtttgttcaa
      661 cgtacccaaa ttgactccct ggccgtagca atcggcacag cacatggttt atatgcgcat
      721 gaaccacgac tggattttgt gcggctggaa cagattcgtc agcgagtgga gattccactg
      781 gtgttgcatg gtgcttcggg cttatcagaa caggacgttc agcactgtat tcgccgcggt
      841 atttgtaaag tgaatgtcgc gacagaattg aaaattgcgt ttgccgatgc gttaaaagga
      901 tatttctaca acaatcctgc ggcaaatgat ccacgccact atatgcagcc cgcaaaggcc
      961 gccatgaaag aagtcgtaac tcgtattatt ggtgtctgtg gtagtgaagg aaaaatttaa
     1021 gacgttgtac cctacaaaaa caatgaggat tgtttatgaa aaatattaaa ttacttggtt
     1081 cagctatgtt ggtgagcctg gcattgttct cgcaaaattc gttggcaaaa gagtataaaa
     1141 ttggtgcgtc attattaaca cagcaacatc ctttctatat tgatcttgcg aatgccatga
     1201 aagaagaggc aaaaaaagac aatgtcaatt taagtgtctc tattgccaat caggatttaa
     1261 acaaacaact ttctgatgtt gaggatttta ttactaaaaa agtggatgcc attattatct
     1321 ctccggtcga ttcgaaaggc gtccaggcgg cgataattaa agccgataag gccggtattc
     1381 ccgttatcac cgtcgacgtc gctgcggaag gcgttccggt cgtatcgcat gttgcgaccg
     1441 ataactatgc gggaggcgtt gaagcaggca agttaatggg caaacttttg aatggtaaag
     1501 gtactgtagc cattatttct tatccggcac tacaatcggt ggttgcgcgc gttgatggtt
     1561 ttaagaaagg gttaagcgat acaccggata ttaaaattgt tgctgaacag cctggtatta
     1621 cgcgggctga agcgttaacg actgcgcaaa acattatgca ggcaaatgcc aacctgaatg
     1681 gtctgtttgg ctttggtgat gatgcggcgc tggcagcggt tatcgcagca aaatctgcgc
     1741 ataacgacaa catcaaaatc attggttttg acggcatgaa agaagcgcgt gatgccgttg
     1801 acagtgaaaa aacgtttgcg gccgtgattc ggcaatatcc cgatcaaatg ggtgctaaag
     1861 ccattgatgc cgctgttgat cacctcaatg gtaaaccggt agaaaaaatg atcccggtac
     1921 aacccggcgt ttatacaggt aaatagtaaa catcttcacc tcacaacaaa acgttattgg
     1981 cgttttgttg tgacaccgag gtcattatga cggatgttat tcttgatgtt tcgcatattg
     2041 cgaagacatt tggtcatgtt caagcattaa aagatattac cctctcttta cgtaaagggc
     2101 gggtgcatac tttattaggc gagaatggtg ccggtaaatc gacattaatg aaaatactgg
     2161 ctggtgttta tccgccaacc cagggaacga taacactacg tggtgaaact atcaccatta
     2221 ataatccgca gcattcacgc cagttaggaa ttgcgattat ttttcaggaa ttgagcctga
     2281 gtaataatat gaccgtggcg gaaaatattt atgccaataa tgaaccgcga cgttttggca
     2341 ttattaacga taaaaaaatg ctcgcagatt gccagaatct cctcgcggaa ctgggcatac
     2401 cactagaccc actagaaatg gtcggcaata tgtcgatggc acataggcaa ctggtagaaa
     2461 ttgctaaagc cttaagctat gcagcggatg tcgtgattat ggatgaaccg acatcttcgc
     2521 taagtgataa tgaagcggag attctgttta acattattga aaaattaaag cagcgaggct
     2581 gtgcggttat ttatatctct catcgcatgg atgaaattat gcggatttca gatgatattt
     2641 cggtgatccg cgacggtgag tatatcgcca cgcatgaaaa gaaaaacagt gatatccagc
     2701 atttgattgc ccaaatggtt ggtcgggaaa tgaaaaatat ttggccagca cgtcttgggg
     2761 aaataccgga tgaaaatgtt cctgcaaaac tggaagtaaa aaatctttcc catccttcgc
     2821 tttttaaaga gatcagtttc gctgtgcgtc cgggggaggt cctcggattt tttggcctgg
     2881 tgggggccgg gcgttccgat gtgatgaagg cgctctttgg cctcgtttcg tatcagggga
     2941 ctgtgttgat tgatggtaag gaagtcagga tcgctaatcc taaacaggct attgatcatg
     3001 gcattgcttt tgtgaccgaa aaccgcaagg aagaagggtt agtgctgatg cacgacgtca
     3061 atatgaacac gcaccatgtg gcgtttcaat ataacgcttc gcggatggga ctcatcaatc
     3121 accgacaaga agaggcgaag acgctgcaat caattgccag aatgaacact aaagtgagtt
     3181 ctgtacatca ggcagtggga gcgctgagcg gcggtaatca acagaaaatc gtgttatcga
     3241 agtggctgga gaaaactccg cgtattttat tgctcgatga gccaacgcgc ggagtggatg
     3301 tcggcgcaaa atttgaaatc tataacgtga tacgtcaact ggctgcggca gggacagcaa
     3361 ttattctggt gtcctctgaa ctgccagaag tcatggcact tagcgatcgt ctggtggtaa
     3421 tgcgtaataa aacgattgcc gatatttatt catgtgaaaa tttaacgcag acacaggtaa
     3481 tgaccgctgc aacaggagta cgttaaatga cagagaaaac tcttgccagt ccgaaacctt
     3541 cggaaattcc cattgcaaat aattttaaaa aagtcttcag gcaatatggc ggtatcctca
     3601 gtggaatggt ggtgttaatc attctgttca gttttattaa tgacagtttt tttaccgcca
     3661 acaacattac taatattatt cttcaggttt cgataattgc aattaccgcc tatggaatga
     3721 cttatgtact tttgcttggc gatattgatt tatcggtcgg ttcaaccatt gcattgattg
     3781 gcacatttgc tgcgcttggg gcttcatggg gaattccatt tattttattg gtcccattat
     3841 ccattattgc agcactggct ctggggatga taaatggtgg cttgacggct atagcgggga
     3901 tcccgtcgtt tattgtcact gttgctacta tggggatctt ccgtggtatc gcttatatag
     3961 tgaccgatgg tatgcccatc atgattaaag atgatgcctt tctggcactg gggaatggag
     4021 aatttctcta tatacctatt ccaatctgga tattaatcat cttattgctg attaaccact
     4081 tcattcttac taaaacaacc tttggacgaa aaatttatat cactggtggt aataaagaag
     4141 cggcaattta ttcagggata aatgtcaccc gcttgaaaat taaagtcttt atgattacag
     4201 ccgtgctggc aggtatcagc gggatgatcc tcgcctcacg ccgctattcc gggcaaccga
     4261 attccgcgtt gagctacgaa ctggatgcta ttgctgcggc tgtactggga ggcaccagcc
     4321 tgaacggcgg ctatggaacc gtggtgggga cggtgatcgg cgcattaact atcggcgtta
     4381 ttaataatgg catgaactta atgaatgttc cctattttta ccagatggtg gttaaaggat
     4441 tagtcattct tgtagccgtt tattttgacg taagaaataa aagaaaacgc agctaatcat
     4501 ttcaaggaat taatcatatg agtaaggtct tcacaattgg agaaattctg gttgaaatta
     4561 tggcgagtaa aatcggccag ccatttgatc aaccaggtat ctggaacggc ccttatccca
     4621 gtggtgcgcc cgctattttt atcgaccagg tgacacgtct tggcgttccg tgcggcatca
     4681 ttagctgtgt ggggaatgat ggctttggtg atattaatat acatcgtctg gctgctgacg
     4741 gtgtggatat cagggggatt tcagtcttac ctctggaagc taccgggagt gcctttgtta
     4801 cttatcataa ctctggcgat cgcgatttta tttttaatat taagaatgcc gcttgcggta
     4861 aattatcggc gcagcatgtt gatgaaaata ttctgaagga ctgtacccat tttcatatta
     4921 tgggctcttc cctgttttcg tttcatatgg tagacgcagt aaaaaaagcg gtaactatcg
     4981 ttaaagcaaa tggtggtgtt atttctttcg accctaatat tcgtaaagag atgctcgata
     5041 ttccagaaat gcgtgacgcc ctccactttg ttcttgagtt aacggatatc tatatgccca
     5101 gcgagggcga agtgttgctc ttgtcgccac attcgacgcc ggagcgtgca atcgcaggtt
     5161 ttcttgaaga aggtgttaaa gaggtgattg tgaagcgcgg caatcaaggt gccagttact
     5221 actccgcgaa tgagcagttt catgttgaga gctatccggt ggaagaagtt gacccgaccg
     5281 gtgcgggaga ctgctttggt ggcgcgtgga ttgcctgccg ccagctaggg tttgacgccc
     5341 atcgagcgct gcaatatgcc aatgcctgcg gtgcgctggc ggtaaccaga cgtggcccaa
     5401 tggagggaac gtctcgcctt gcggaaatcg aagcgttcat ccagcgccat gacatgtcca
     5461 ttcgggaagc agcacaatga aaaacgaaat ttggtcgtgg attggcgatc tctcgcaggt
     5521 tgccggaatt aaacactatg agttacgtag tggcagagca aagggtacag aagcgtttga
     5581 tgtccgtact ggtgcgggac tggcatttac ggtggtaaaa gatcgcgcgc tggatattgc
     5641 atgggccagc tacaaggaca cggcgctttc ttttatcacc cccaacggca ttgtcgcccc
     5701 cgcttttttt gagtctcagg gcaacggatt tttacgtagc ttttacgcgg ggttactgac
     5761 tacctgcgga cttagctaca ttggcacgcc ttgtgaggat gaaggagaaa cgctggggtt
     5821 acacgggcgt ttggctgcga ctccagcaga agaggtgggg tatcgcacag aacggaccga
     5881 cgacggcatt gaatttgtca tcaatggcaa agtacgggaa acgcgactgt tcggcgaaaa
     5941 tctgacgctg gaaaggacca ttcgctgccg ttatggcgaa aatgtgctgc gcattgaaga
     6001 caaggtgact aatcatggtt tcacccgcca gccgctgcaa attctttatc actttaatta
     6061 cggctggcct ttattatcgc cacaggcaga aatcttgctg tcggctaaaa gcgtaacgcc
     6121 acgaactccg catgcggcgg aagggttaac cagtcatctg gagatttgta ccccacagcc
     6181 cggttttgac gagcaggttt actatctcac gttgaatagc gacagtcagg ggatgagtaa
     6241 agttgctctg gtgaacgccg aactggggtg gggaatttac gagaaattcg acaccatgca
     6301 gctacccaac tttatccagt ggaaaaattt aggcgcgggt gagtatgtta tgggactgga
     6361 ggtcagtaat agttttcctg acggtcgtga taaagaacga gcgcaggggc gtctgccctt
     6421 tattgagcca ggggaaacaa agaaatactg ctttgagctg ggaattgttg atggcgatgc
     6481 tgaaatgagc gcgttgaaag cggaaattgc aggctaccgt tagctaacgg caataaaaac
     6541 gccgggcagc gttaacaggc ccggcgttga ttacgttctt tgctaagcgc gatgaaaaca
     6601 ccgcgcgtca caaattattt ttcctgaaga ccgagttttt tctccagata gtggatgtta
     6661 gtgccaccat gctggaagtt ctcgtcattc atgatgcgga tctgcagatc aacgttggtt
     6721 ttgataccgt cgatgatcag ctcctgcagc gcattcttca tgcgggcaat cgccacgtca
     6781 cggttttcac cgtagcaaat cagcttaccg atcattgagt catagtacgg cggtacggtg
     6841 tagcccgcgt agatatgaga ctcccaacgt acgccaaaac cgccaggtgc gtggaaacgg
     6901 gtgattttgc ctgggcttgg caggaaggtg ttcggatctt cggcgttgat acgacattcc
     6961 accgcatggc cgcgaacgtg aacttcttct tgcttgatcg acagcggttg accggcagcg
     7021 atacgcagct gttctttgat caggtcaacg ccggtgatca tttctgtaac cgggtgttct
     7081 acctgaatac gggtgttcat ttcgatgaaa tagaactcgc cgttttcgaa caggaactcg
     7141 aaagtacctg caccgcgata gccgatatca acacacgctt tagcgcaacg ttcgccgatg
     7201 tagcgacgca gttccggggt aatgcccggt gctggtgctt cttcgaccac tttctggtgg
     7261 cggcgctgca tggagcagtc acgttccgcc agatagatag cgttgccctg accgtcagcc
     7321 agtacctgaa tctcgacgtg gcgaggattt tccaggtatt tctccatgta aaccatatcg
     7381 ttgctgaaag cagctttcgc ttccgcacgg gtcatggaga tggattgtgc cagttcagcg
     7441 tcgccgcgca ctacgcgcat accgcgacca ccgccgccgc cggaggcttt gataatcacc
     7501 ggataaccaa tgcgtttagc aatggcacgg tttttatcca tatcgtcgcc caacgggccg
     7561 tcagaacccg gtacgcaagg gacgcccgct tttttcatcg ccgcgattgc ggatactttg
     7621 tcgcccatca ggcgaatggt ttctgctttc gggccgatga agataaagcc ggagcgttca
     7681 acctgctcgg caaagttggc gttctcggag aggaagccgt aacccggatg gattgctact
     7741 gcgccggtga tttcagcggc gctgatgatt gccgggatgt tcagataact ttttactgac
     7801 ggagcagggc caatacagac cgtttcatct gccagtaata cgtgttttag atcgcgatcc
     7861 gcgctggagt gcacagcgac agtcttgatg cccagttctt tacaggcacg aagaatacgc
     7921 aatgcaatct cgccgcggtt ggcaataaca attttatcca gcatgttcgc ctcgttactc
     7981 gatgacgacc agcggctcgt caaattctac cggttgtcca ctttcgacca gaattgcttt
     8041 cacggtaccg gatttgtccg cttcgatctg gttcatcatt ttcatggctt caacgatgca
     8101 cagggtatcg cccacgttga ctttctgacc cacttcgatg aacgcttttg cgtccgggct
     8161 tggggtgcgg tagaaagtac caaccatcgg ggaacgtacg atgtgaccac tgatttccgc
     8221 tgctgctggc gcttccatgg aaggaacggt cgccggagcg gctgcgttag attgagctgg
     8281 ctgctgcatt attggtgcag cgtaagcttg ttgcatcaca gggaaacttg cggcaggagc
     8341 tgcacggcta atgcgtactg actcttcgcc ttcagaaatt tccagttcgg agatgcctga
     8401 ttcttcaacc agctcgatca gttttttaat cttacgaata tccatgagtg ggttccgtac
     8461 tctttgttta gtgtgattgt gacaggcgtt tcaccgccgt ctgtaaagcg tatgccccga
     8521 cagctgtatg catagcgata aattccagca ggccgggacg ccggtctatt ttgccccaga
     8581 gtgtcaatgt tagacttgac ggacattgtg cagcgatctg ctgctatcct ccggcaaaaa
     8641 acaaaatata ccttcgttgc gccaatgtca ccttttccgc agcgcaaaaa ctgcgtcagg
     8701 gaggacgagg tcgcacatta taacgatttc gtagcaattg gcagctaaat actggtctta
     8761 tcagggaaga taatcaacag ctaacatgta aataaccttc aacaccgtgt aatttgcaac
     8821 aagccgcaca attcacgaaa ttagcgccat catcgacgga acttcttata acgtaaggtg
     8881 aaaagctgta aagccagccc tgcgaagatg aggggctgca atgagataat cttcacagac
     8941 cacaaataat gtagggatgc caggatcgcg acaagataga cgacattacg cagcggttgc
     9001 tggggattac ccaattttca ctgcattgtc tggatagatg ttaatgctaa agcacgcaaa
     9061 attaaccgat tgacaatgct cagcattaaa taggttcgag tgattaattt gtagtcaaac
     9121 aaaatcagat tattcccccc attttgcaga aggtaaactg ttttgtcagg cggcagtgcg
     9181 gctatctgat gctttcagaa tttgccacct gacaataaaa aagccgattt ggtagcaaaa
     9241 tcggcttcgg tgagtttgca gttttcatca catactccga caagcttttt caagcatgac
     9301 ggaggttgcg ttaatgcaag gtttctaccc agggataaaa gtgaccgtta cggcggatga
     9361 atatctgtgt tgtcagagga tggtcatcct cacggaagca ttgttttgtt agctgtcgta
     9421 atgcactgag atttgctggc tgatcattgt gggtgaaaaa cagttttcct ctggagggaa
     9481 cggtcactaa caggtcgccg cattcaggat cgggtgattg cacccaaaga tcgtcaatga
     9541 gcatcagact gggctcataa gtaccccctg cggagataac ggcaactttt ccatcgtttg
     9601 cgcctgtatc aacgatttca gccgtaactt ccggcaatat ttgcttcaaa ttactgatgg
     9661 cttgttcgcg gtcaatggtc acttcgtagc gcatcagata gcgcatggtt tcgctaccat
     9721 cttcagcaaa caagataatt aactcatcgt taaagtcttc ccaggcaaaa tccagcgagg
     9781 cgttgaggac ctctggatca gactcatact ggctaagctg ttggcgaacg gtatccagcc
     9841 aggctttttc tttaatcacc ggaacaatac agcgcccggt cggcagcatt tctgcaagct
     9901 gtttcagcat atctgcagcc acttcttgca gcgggtctgg tgcggcttga taacgtttgt
     9961 aggcatcatc aagggtgatt tccaccgttt ggtgacccgt atcaatgcgc atcaatttgt
    10021 ggtcagtcga aatcatgact ttccagtttg gatgatgttg tacaacttcc tgaccaaggt
    10081 gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa ccgattgaat aatgatgtca
    10141 aaatgagttc cctctcttat tattccttgc taaatggtta gttaaccttc accagcgtgc
    10201 gaccctggat ctggttatta atgatggcct cggcaaagtt cggtgcctct gacagagata
    10261 tctcttttgc cgcctgggta tagaatgatt ccggtaaatc ggcgaccagt cgctgccagg
    10321 cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac cccttgcaaa cggacattac
    10381 gcagaataaa tggcatgacc gtggttggca gagtaaaacc acccgccaga ccacaggcag
    10441 ccacgcagcc gccgtaattc atttgcgcca gcacttttgc cagcactttg tcgccaacag
    10501 tgtcaattgc cccagcccag acttgttttt ccagaggacg ggattcggca aactcatcac
    10561 gagggagaat acgactggca cctaaacttt tcagatattc atgggtactt tcgcgaccgg
    10621 aaacggcaac gacctgataa cccaacttat gcagcagcgc cacggcggta ctgccgacgc
    10681 caccactggc acccgtcacg acaatctccc cgtcctgcgg gcgaacaccg gcatcttcca
    10741 gcgccatcac acacagcatg gcggtaaaac cggcagtacc gataatcatt gctttacgcg
    10801 cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt cactcgcgcc tgctccgcca
    10861 gcccacccca gtggttttca ccaacgcccc agccagtgag taacacctcc tgaccggcat
    10921 gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa atcgatccca ggaatcatcg
    10981 gaaaattacg gatgattttt cccttaccgg taatcgccag cgcatcttta tagttcaggc
    11041 tcgaccagtg aacatcaacc gtgacatcgc cctccggcag gcgactttcg tcaagtgtct
    11101 gtaccgatgc gagagttttg ccgtcctgct gttctaaaag taacgcctgc ataagtggtc
    11161 ctcatgtgca tgatggattg gaaaataatt tctgaagact atactcgcta atggaaaagc
    11221 aaagccgatg aagcgcaata aattgcgaga taaatctgat ttgctagtat gcccgcttcc
    11281 tcactatcgg agttaacaca aggatgagat taacgacgaa attttcggcc tttgttacgc
    11341 tgctcaccgg gttaacaatt tttgtgactt tgctgggctg ttcgctaagt ttctacaacg
    11401 caattcagta taagtttagt catcgcgttc aggcggtggc gacggcgatt gatacccacc
    11461 ttgtgtcgaa tgacttcagc acattaaggc cacaaattac cgaattaatg atgtcggcag
    11521 atatcgttcg tgtagacctg ctccatggtg ataaacaggt ttataccctg gccagaaatg
    11581 gtagttatcg tccggttggc tccagcgatc tgtttcgcga actgagtgtt ccgttgataa
    11641 agcatccggg gatgtcgctg cgtctggttt atcaggatcc gatgggcaac tatttccatt
    11701 ctttaatgac taccgcgccg ctcacggggg cgattggctt tatcattgtt atgctcttcc
    11761 tggcggtacg ctggttacaa cggcaacttg ccgggcaaga attgctggaa acccgggcta
    11821 ctcgtatctt aaacggtgag cgtggctcta atgtgttggg aaccatctat gaatggccgc
    11881 ccagaaccag cagtgcgcta gatacgctgc ttcgtgaaat tcagaacgca cgcgaacaac
    11941 acagccgtct tgatacgctg atccgctctt atgccgccca ggacgtgaaa accggcctca
    12001 ataaccgact ctttttcgat aatcagttag caacgttact ggaagatcag gagaaagtag
    12061 gtacccacgg gatcgtgatg atgattcgtc tgccggattt caatatgttg agcgatactt
    12121 gggggcacag ccaggttgaa gaacagttct tcactctgac gaatctgctg tcgacattta
    12181 tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg cagtgatttt gctgcgctgt
    12241 taccgcaccg gacgttaaaa gaggcagaga gcatcgccgg tcagttaatc aaagccgtcg
    12301 ataccttgcc gaacaataaa atgctcgatc gcgacgatat gatccacatt ggtatctgcg
    12361 cctggcgtag tggtcaggat accgagcagg taatggaaca tgcagagtct gccacgcgta
    12421 atgcgggatt gcagggcggc aatagctggg ctatttacga tgactcgttg cctgaaaaag
    12481 gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat gctcagtcgc ggcggcccgc
    12541 gcctttatca aaaaccggcg gttactcgcg aaggtcaggt tcatcatcgc gaactcatgt
    12601 gccgcatctt cgatggtaat gaagaggtta gctcggcgga gtatatgccg atggtcttgc
    12661 agtttggctt atcggaagag tatgaccgtc tgcaaatcag ccgtcttatt ccactattgc
    12721 gttactggcc agaggaaaat ctggcgattc aggttaccgt tgagtcgctg attcgcccgc
    12781 gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga aaaatcacaa cgaaaacgca
    12841 taattattga acttgcagag gccgatgtag gtcaacatat cagtcgttta caacctgtta
    12901 ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa ccaggctggt ttgacgctgg
    12961 taagcaccag ttggatcaaa gaacttaatg ttgagttact caagctccat ccggggctgg
    13021 tcagaaacat tgagaagcga acggagaacc agctgctggt tcaaagcctg gtggaagcct
    13081 gctccgggac cagcacccaa gtttacgcca ccggtgtgcg ttcgcgaagc gagtggcaga
    13141 ccctgattca gcgcggtgtt acaggcgggc aaggggattt tttcgcgtcc tcacagccac
    13201 ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt ttaacctgcc gtttaatctg
    13261 ttttcacgta gaataacgcg cgctgcgtct catgggagtg tgcttgtctg ctcgccagat
    13321 tgttgcagca catatgcaga tgaatgacct tacgcggttg caaacaggcg aggaatgctg
    13381 ctgatgcatt aagcctttct ggactcaggc agagatttgt aacaaaggaa acgaactgca
    13441 ctaattttca ccgtagcaga tgatttttgc gccttgtcgc tgctgcgtgt ggttggtaaa
    13501 gtaagcggat tttcttttcc gccccagctt tcaggattat cccttagtat gttgaaaaaa
    13561 tttcgtggca tgttttccaa tgacttgtcc attgacctgg gtactgcgaa taccctcatt
    13621 tatgtaaaag gacaaggcat cgtattgaat gagccttccg tggtggccat tcgtcaggat
    13681 cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg acgcgaagca gatgctgggc
    13741 cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag acggcgttat cgccgacttc
    13801 ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag tgcacagcaa cagctttatg
    13861 cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg cgacccaggt tgaacgccgc
    13921 gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag tcttcctgat tgaagaaccg
    13981 atggctgccg caattggtgc tggcctgccg gtttctgaag cgaccggttc tatggtggtt
    14041 gatatcggtg gtggtaccac tgaagttgct gttatctcct tgaacggcgt ggtttactcc
    14101 tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta tcatcaacta tgtgcgtcgt
    14161 aattacggtt ctctgatcgg tgaagccacc gcagaacgta tcaagcacga aatcggttcg
    14221 gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg gccgtaacct ggcagaaggt
    14281 gttccacgcg gttttaccct gaactccaac gaaatcctcg aagcactgca ggaaccgctg
    14341 accggtattg tgagcgcggt aatggttgca ctggaacagt gcccgccgga actggcttcc
    14401 gacatctccg agcgcggcat ggtgctcacc ggtggtggcg cactgctgcg taaccttgac
    14461 cgtttgttaa tggaagaaac cggcattcca gtcgttgttg ctgaagaccc gctgacctgt
    14521 gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca tgcacggcgg cgacctgttc
    14581 agcgaagagt aatcggatgc aggcagggga aatgtctgtt taccctgcct ggtctgatac
    14641 gagaatacgc ataacttatg aagccaattt ttagccgtgg cccgtcgcta cagattcgcc
    14701 ttattctggc ggtgctggtg gcgctcggca ttattattgc cgacagccgc ctggggacgt
    14761 tcagtcaaat ccgtacttat atggataccg ccgtcagtcc tttctacttt gtttccaatg
    14821 ctcctcgtga attgctggat ggcgtatcgc agacgctggc ctcgcgtgac caactagaac
    14881 ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa cagtgaactg ctgatgcttg
    14941 gacaatacaa acaggagaac gcgcgtctgc gcgagctgct gggttccccg ctgcgtcagg
    15001 atgagcagaa aatggtgact caggttatct ccacggttaa cgatccttat agcgatcaag
    15061 ttgttatcga taaaggtagc gttaatggcg tttatgaagg ccagccggtc atcagcgaca
    15121 aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac cagtcgcgtg ctgctgattt
    15181 gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa cgatatccgc gtaattgcag
    15241 ccggtaacgg ttgtacggat gatttgcagc ttgagcatct gccagcgaat acggatattc
    15301 gtgttggtga tgtgctggtg acctccggtc tgggcggtcg tttcccggaa ggctatccgg
    15361 tcgcggttgt ctcttccgta aaactcgata cccagcgcgc ttatactgtg attcaggcgc
    15421 gtccgactgc aggactgcaa cgtttgcgtt atctgctgct gctgtggggc gcggatcgta
    15481 acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt tgctaatgaa cgtctgatgc
    15541 agatgatgcc gcaggtattg ccttcgccag acgcgatggg gccaaagtta cctgaaccgg
    15601 caacggggat cgctcagccg actccgcagc aaccgacgac aggaaatgca gctactgcgc
    15661 ctgctgcgcc cacacagcct gctgctaatc gctctccaca aagggctacg ccgccgcaaa
    15721 gtggtgctca accgcctgcg cgtgcgccgg gagggcaata gtggcgagct atcgtagcca
    15781 gggacgttgg gtaatctggc tctctttcct cattgcgctg ttgctgcaaa tcatgccctg
    15841 gccggataac ctgattgttt tccggccaaa ctgggtgtta ctcatcttgt tgtattggat
    15901 cctggccttg cctcatcgcg taaatgtggg cacaggtttt gtgatgggtg ccatactgga
    15961 tctgatcagc ggctcgacgc ttggcgtacg cgtattggcg atgagcatca ttgcttacct
    16021 ggtggcgctg aaataccagc ttttccgcaa cctcgcatta tggcagcagg cgctggtcgt
    16081 catgttgctt tcgctggtgg tggatattat tgttttctgg gcagagtttt tagtgattaa
    16141 cgtctctttc agaccggaag tgttctggag tagtgtagtc aatggggtgc tctggccgtg
    16201 gattttcttg ctgatgcgta aagtccgtca gcagtttgca gtgcaataaa ggtttctatg
    16261 acttctctgt atttagcttc cggttctccg cgtcgtcagg agttacttgc gcaacttggc
    16321 gtgacctttg aacgtattgt tacgggcatt gaggagcagc gtcagccgca ggagagcgcg
    16381 cagcagtatg ttgtgcgtct ggcgcgcgag aaagcacagg cgggtgtcgc gcaaacggcg
    16441 caggatctcc cggtgctggg tgcggatact atcgttatcc tgaatggaga agtgctggag
    16501 aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca aattatcggg tcagacccat
    16561 caggtgatga cggcagtggc gttggccgac agccagcaca ttctcgattg cctggtggtc
    16621 accgatgtga ctttcagaac gttaacagac gaagacatcg cgggctatgt cgccagcggt
    16681 gaaccgttag ataaagcagg tgcatacggt attcaggggc tgggtggctg ttttgtcagg
    16741 aagataaatg gcagctatca cgccgtagtc ggcttaccgc tggttgaaac gtatgaatta
    16801 ttaagtaatt ttaacgcact gcgtgagaaa agggataaac atgacggctg aattgttagt
    16861 aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat ggcggtattc tgcaggaaat
    16921 tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat atctacaagg gtcgtgtaag
    16981 tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt gggctggata aagccgcgtt
    17041 tcttcatgca tccgatatca tgccgcacac cgaatgtgtg gcgggtgaag aacaaaagca
    17101 attcacggtg cgcgacatct cggaactggt tcgtcagggg caagatctga tggtgcaggt
    17161 ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc accgatatca cgctgccttc
    17221 tcgctatctg gtgtttatgc caggggcttc tcacgttggg gtttcccaac gtattgaaag
    17281 cgaatcagaa cgtgaacgcc tgaaaaaagt ggtcgcagag tattgcgacg agcagggcgg
    17341 gtttatcatc cgtaccgcag cggaaggggt tggcgaggct gaactggcct ccgatgccgc
    17401 ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa aaacgcccgc agacccgtta
    17461 tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg cgtgatttcg ccgacgccga
    17521 actggaccgc attcgcgttg actcacgcct gacttacgaa gcattgctgg agtttacctc
    17581 ggagtacatt cccgagatga ccagcaagct ggagcattac accggacgcc agccgatttt
    17641 cgatctcttt gatgtcgaaa acgaaatcca gcgagcgctg gaacgcaaag tagaactgaa
    17701 atccggtggt tatctgatta tcgaccagac cgaagcgatg accaccgtgg acatcaatac
    17761 cggagcgttt gtcggtcatc gcaatctgga cgacaccatt ttcaatacca atattgaagc
    17821 gacgcaggct atcgctcgcc agttacggtt gcgtaatctg ggtgggatta tcattattga
    17881 tttcatcgat atgaataatg aagatcaccg ccgccgcgtg ctgcactcgc tggagcaggc
    17941 gttgagcaaa gaccgggtga aaaccagcgt taatggtttt tcggcgctgg ggctggtgga
    18001 gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta ctgtgtaacg aatgcccaac
    18061 ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta tgctatgaaa tcatgcgcga
    18121 gattgttcgt gtccaccatg cttacgactc cgaccgtttc ctggtctatg catctccggc
    18181 agtagctgaa gccttgaaag gcgaagagtc acactcgctg gcggaagtgg aaattttcgt
    18241 tggcaaacag gttaaagtac aaattgaacc gctctataac caggagcagt ttgacgtcgt
    18301 aatgatgtaa acagatgctg ggccgccatc cggcaaaggg tttttgagtc acatttttag
    18361 cagacaagga gtgacgggtg aggcgattgc cggggatttt actgcttact ggagccgcgc
    18421 tcgttgtgat cgctgctctg ctggttagcg gcctgcgtat tgctttaccg catcttgacg
    18481 cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac tggcatgccg gtagaagcca
    18541 gtcagctctc agccagctgg cagaattttg gcccgacgct tgaagcacac gacatccgtg
    18601 cagaactaaa agatggcggc gaattttcgg ttaaacgcgt tactctggcg ctggatgtct
    18661 ggcagagcct gttacatatg cgctggcagt ttcgcgacct cactttctgg cagctgcgct
    18721 ttcgcaccaa cactcctatc accagcggtg gtagcgatga cagtctggaa gccagtcaca
    18781 tcagcgatct gtttcttcgt caatttgacc atttcgatct ccgcgacagt gaagtcagtt
    18841 ttctgacgcc atccggtcag cgcgccgagc tggcgatccc acaactcacc tggctgaacg
    18901 atccacgtcg acaccgtgcg gaaggcctgg taagcctctc cagccttacc ggacagcacg
    18961 gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg gttgttaagc aatggtcgcg
    19021 tctggctaca ggcggatgac atcgacctga agccgtggct cggtaaatgg atgcaggaca
    19081 atattgctct ggaaacggca cagttctccc ttgaaggctg gatgacgatc gacaaaggcg
    19141 atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc cagctggttg ggcgagaagc
    19201 aaacgcatac gctgtcggtg gataatctga ccgcgcatat tacgcgtgaa aatccgggct
    19261 ggcagttctc tattcccgat acacggatca cgatggacgg caaaccctgg ccgagcggag
    19321 cattgacgct ggcctggata ccggaacagg acgttggcgg caaagacaat aaacgcagtg
    19381 acgaactccg gattcgcgcc agtaatctgg agctggcagg cctggagggc atacgcccgc
    19441 tggccgcgaa actttcacct gcactgggtg atgtttggcg ctccacacaa ccgagcggca
    19501 agattaacac tctggcgctg gatatcccgc ttcaggcggc agacaagacc cgttttcagg
    19561 catcgtggag cgatctggcc tggaagcaat ggaaattatt accgggtgcg gaacacttct
    19621 ccgggacgct ttccggcagc gttgaaaatg gtttgcttac cgcgtcgatg aagcaggcaa
    19681 agatgcctta cgaaacggta ttccgtgcgc cactggaaat cgccgacggc caggcaacta
    19741 taagctggct gaacaatgac aaaggtttcc agctggatgg gcgtaatatt gacgttaaag
    19801 ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca acctgctaac gatgaaccct
    19861 ggctgggtat tctggctggc atcagtaccg atgatggttc acaagcctgg cgctatttcc
    19921 cggaaaactt gatgggtaaa gacctggttg attacttaag tggcgcgatt cagggcggtg
    19981 aagcggataa cgcgacgctg gtttatggtg gcaatccgca actcttcccc tataaacaca
    20041 acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc gaagtttgcc ttccagccgg
    20101 actggcctgc attaactaac cttgatattg aactggactt tattaacgac ggtttatgga
    20161 tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag taatctcacc gcagtgatcc
    20221 ctgactactc gaaagaaaaa ctgctgattg acgctgacat taaaggtccg ggtaaagccg
    20281 ttggccctta ctttgatgag acaccgctga aagattctct gggtgcgacc ctgcaagaac
    20341 tccagctcga cggcgatgtg aatgctcgct tacatcttga tatcccgctg aacggcgaac
    20401 tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag tctgtttatc aaaccactcg
    20461 acagcaccct gaaaaatttg agcggtaaat tcagctttat caatggcgat ctgcaaagtg
    20521 aaccactgac agcaagctgg tttaatcagc cgttgaacgt ggatttttcc accaaagaag
    20581 gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa ctggcaaccg gcgaaaacca
    20641 gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag cgtggcgtgg gatggtaaag
    20701 tgggcattga tctgccttat catgctggcg cgacctataa cgtagagctg aacggcgatc
    20761 tgaataatgt gagtagtcac ttaccttcac cgttagccaa acctgcgggt gaaccactag
    20821 cggtaaacgt taaggttgat ggcaatctca acagctttga attaaccgga caggctggtg
    20881 cggataacca tttcaatagc cgctggttgc ttggtcaaaa gctgacgctc gatcgtgcta
    20941 tttgggcggc agacagtaaa acgctcccgc cgttgccgga acaaagtggc gttgaactca
    21001 atatgccgcc gatgaatggt gccgagtggc tggccctgtt ccagaaaggc gctgcggaga
    21061 gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt acgtacgcct atgttgtcac
    21121 tgggaaatca gcaatggaat aacctgagta ttgtttcgca accgacggca aatggcaccc
    21181 tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc gatgcgtaat aacgcgccgt
    21241 ggctggcgaa tatcaaatat ctttattaca acccgagcgt ggcgaaaact cgtggtgatt
    21301 caacgccgtc atcacctttc ccgacaacgg agcgcattaa cttccgtggc tggccggacg
    21361 cacaaatacg atgcacagag tgctggttct gggggcaaaa attcggtcgc attgacagtg
    21421 atatcaccat ttctggcgat acgttaacgc tgaccaatgg actgattgat actggtttct
    21481 cgcggcttac tgccgatggt gaatgggtta ataatccgga gaatgaacgt acctcgctga
    21541 aaggaaaact gcgcgggcag aaaattgatg ccgccgcaga attttttggt gtcacgacgc
    21601 ccatacgcca gtcgtcattt aatgtggatt acgatttaca ctggcgcaaa gcaccctggc
    21661 aaccagatga ggcgacgttg aatggcatca ttcatactca actgggtaaa ggcgaaatta
    21721 ccgaaatcaa taccggacat gccgggcaat tgctgcgctt attgagcgtt gatgccctga
    21781 tgcgtaagct gcgttttgat ttcagagaca cttttggcga agggttctat tttgactcca
    21841 ttcgcagcac cgcgtggatt aaagacgggg ttatgcacac cgacgacacg ctggtggatg
    21901 gcctggaggc ggatatcgcc atgaaagggt cggtaaatct ggtacgtcgc gacctgaata
    21961 tggaagcggt tgtcgcacca gagatttctg cgacggtggg cgtggctgcg gcttttgcgg
    22021 ttaaccccat tgttggcgcg gcagtatttg cagccagtaa agtgctgggg ccgctgtgga
    22081 gcaaagtctc cattttgcgc tatcacattt cgggtccgct ggacgatccg caaatcaacg
    22141 aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt tgacgagggc gcgtaattgc
    22201 cccaatctca tatgataatc gttgccaaag gccaacgagc cggaacataa ctgtaggccg
    22261 gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc ctctactgca gtaactaacg
    22321 agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa ttgctagcgg cgaacggcct
    22381 gaaacatcag gacttgttcg cgatcctcgg tcaactggcc gaacgtcgcc ttgattatgg
    22441 cgatctctat tttcagtcga gctatcacga atcctgggtt ttagaagacc gcattattaa
    22501 agatggttct tacaacatcg atcagggcgt tggtgtgcgt gcaatcagtg gtgaaaaaac
    22561 cggatttgct tacgctgacc aaatcagcct gctggcgctg gaacagagtg cgcaagcggc
    22621 gcgcaccatc gtccgtgata gtggtgatgg caaagtacag acgctgggcg cggtagagca
    22681 tagcccgttg tatacctcgg tagatccgct gcaaagcatg agccgtgaag agaagctgga
    22741 tatcctgcgt cgcgtcgata aggttgcccg cgaagcggac aagcgcgtac aagaagtgac
    22801 tgccagcctc agcggcgttt atgaattaat tctggttgcg gccaccgacg gcacgctggc
    22861 ggcggatgtt cgtccgctgg tgcgtctttc cgtgagcgtt ctggtcgaag aagatggcaa
    22921 acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt tatgagttct tccttgccga
    22981 tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa gcagtgcgta tggcgctggt
    23041 caatctttct gccgttgctg caccagcggg caccatgccg gtagtacttg gcgcaggttg
    23101 gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg gaaggcgact tcaactgccg
    23161 cggcacttca gtatttagtg ggcaggtcgg ggagctggtg gcttcagaac tgtgtaccgt
    23221 ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg gcgattgatg acgaaggtac
    23281 gccaggccag tacaacgtgc tgattgagaa cggcattctg aaaggctaca tgcaggataa
    23341 actcaacgcg cgtttgatgg ggatgacgcc gactggcaac ggtcgccgtg aatcctacgc
    23401 ccatctgccc atgccgcgta tgaccaacac ctatatgctg ccgggtaaat cgaccccgca
    23461 ggaaattatt gaatccgttg agtacggtat ctatgcaccg aactttggtg gcggtcaggt
    23521 ggatattaca tccggcaaat tcgttttctc cacttcagaa gcgtatctga ttgaaaacgg
    23581 taaagtaacg aagccggtga aaggcgcgac gttgattggt tccggtatcg aaaccatgca
    23641 gcagatttcg atggttggca acgacctgaa actggataac ggcgtgggtg tctgcggtaa
    23701 agaagggcaa agtttgccgg ttggcgtggg ccagccaacg ctgaaagttg ataacctgac
    23761 tgttggcggt actgcgtaat aatttcatta ttttcagaag gataattaat ctttctacgt
    23821 gcacgaacgg tcccctcgct cctctggggt tagggtgagg ggaacccgtt ggcacaggtt
    23881 tgtagaacgt aacagtacaa tatgaattac ttctctttcc cgcgcccgtg catctcctga
    23941 aacaatttac cgacctcaac aaaataatcc gtcagcgagt tgatcacgac ctgtaccttc
    24001 agcggcagct tatctttttc ggtatataac gcataaaccg ggcgtggatc tgactgataa
    24061 cgcggcagca ggatctccag ctcaccgcga ttgatctcgt tgatcaccca catcagcggc
    24121 acgtaggcga tcccggcacc cgccgttagc cagcgcacca gcgtcatcgg atcattagtc
    24181 acaaatctcc cttgcgggat caggcgagtc gagatccctt ccggtgcaat cagttcaaat
    24241 tcattgtcgg gccgcacgct gtattcaagc catgaatggc tacttaaatc ggcgggtttt
    24301 tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc acaccaccat tggcatcgcg
    24361 ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc cgacgcggat caccacatcc
    24421 agaccgtcgg caatcaggtc gggggctgga attccggtaa ccagattgac gctcaaacct
    24481 gggtattctt tcagcatttt ggctgtcagc ccggcgagaa cattttgtgc catagttgaa
    24541 gaacagccaa tgcgtagcgt cccgatgggg gtgttattga aggcatacag ttgctcatga
    24601 acatcctgca cttcatgaag catacgacgg cagccctggt agtaaattct accggcttcg
    24661 gtcaggccaa tgctgcgtgt gctacggttt aacagcttta cctgcaactc atcttccagt
    24721 tttgacaccg tctgactgat ggacgaaacg ctcatctgta gctgtctggc ggcggcggta
    24781 aaagagccaa attcaactac tttggcaaac accgacatgc gttttagtcg ttccattatt
    24841 cactctgact taaaagtgat ttagatcaca taatatagat aacagcataa cagttacgct
    24901 aatatattaa atatcaatct acagcaatgt tgctctcgcc cggctttcca tgcccttctc
    24961 tgcggcgaca gatgctgaaa ataataacgc ctgctctctc tatacaacca aggtcaacat
    25021 gagtctgttt cccgttatcg tggtgtttgg gctgtccttc ccaccgatat tttttgaatt
    25081 gcttttatca ctggcgattt tctggctggt gcgccgggta cttgtgccaa caggtatcta
    25141 cgactttgtc tggcatccgg cgttgttcaa caccgcgctc tattgctgct tgttttattt
    25201 gatatcgcga ctgttcgttt gaggttgaag tgaaaacact aataagaaaa ttctcccgta
    25261 cggccatcac ggtcgtatta gtcattctgg ccttcatcgc aatttttaat gcctgggtct
    25321 attacaccga atccccctgg acgcgtgacg cgcgctttag cgctgacgtc gttgcgatcg
    25381 cgccggacgt ttctggactc attacccagg tgaatgttca tgataaccag ctggtgaaaa
    25441 aaggacagat actgttcacc atcgaccagc cgcgctatca aaaggcgctt gaggaagcgc
    25501 aagccgatgt tgcctattat caggtactgg cacaggagaa acgccaggag gccggacgtc
    25561 gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat cgaccaggct aacaacgtac
    25621 tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac ccgcgatctg gcaaaactgg
    25681 atcttgaacg cacggtgatc cgcgcgccag cagatggctg ggtgaccaac ctcaacgtct
    25741 ataccggtga gtttattact cgaggatcaa cggcggttgc gctggtgaaa cagaactcct
    25801 tctatgtact ggcctatatg gaagaaacta agctggaagg ggtgcgtccg gggtatcgtg
    25861 cagagatcac gccgcttggc agtaacaaag tgctgaaagg gactgttgat agtgttgccg
    25921 ctggggtcac caacgccagc agcacgcgtg acgacaaagg gatggcgacc atcgactcta
    25981 accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg tattcgtctc gacaaccagc
    26041 aagagaacat ctggcctgcg ggcaccactg ctacagtggt ggtcactggc aaacaagatc
    26101 gcgacgaaag ccaggattcg ttcttccgta aaatggccca tcgcctgcgt gagtttggtt
    26161 aatcacgatg ggtattttct ccattgctaa ccaacatatt cgctttgcgg taaaactggc
    26221 gaccgccatt gtactggcgc tgtttgttgg ctttcacttc cagctggaaa cgccacgctg
    26281 ggcggtactg acagcggcga ttgttgccgc cggtccggcc tttgctgcgg gaggtgaacc
    26341 gtattctggc gctattcgct atcgtggctt tttgcgcatc atcggcacat ttattggctg
    26401 tattgccgga ctggtgatca tcattgcgat gatccgcgca ccattattga tgattctggt
    26461 gtgctgtatc tgggccggtt tttgtacctg gatatcctcg ctggtacgaa tagaaaactc
    26521 gtacgcgtgg gggctggccg gttataccgc gctgatcatt gtgatcacca ttcagccgga
    26581 accattgctt acgccgcagt ttgccgtcga acgttgtagc gagatcgtta tcggtattgt
    26641 gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg atcaaacaag aagtggatcg
    26701 agagctggaa agtttgctgg tcgcgcaata tcaattaatg caactctgta tcaagcatgg
    26761 cgatggtgaa gttgtcgata aagcctgggg cgacctggtg cgacgcacca cggcgctaca
    26821 aagcatgcgc agcaacctga atatggaatc ttcccgctgg gcgcgggcca atcgacgttt
    26881 aaaagcgatc aatacgctat cgctgacgct gattacccaa tcctgcgaaa cttatcttat
    26941 tcagaatacg cgcccggaat tgatcactga tactttccgc gaattttttg acacgccggt
    27001 agaaaccgcg caggacgtcc acaagcagct caaacgcctg cggagagtta tcgcctggac
    27061 cggggaacgg gaaacgcctg tcaccattta tagctgggtc gcggcggcaa cgcgttatca
    27121 gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac gccaccgaag aagagatcct
    27181 gcaaggcgaa ccggaagtca aagtagagtc agccgaacgt catcatgcga tggttaactt
    27241 ctggcgaacc acactttcct gcattctggg cacgcttttc tggctgtgga cgggctggac
    27301 ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg tcactggcaa tgcgtttgcc
    27361 gaatccacgc atggtggcga tcgactttat ctacgggacg ctggccgcgc tgccgttagg
    27421 gctgctctac tttttggtga ttatccctaa tacccaacag agcatgttgc tgctgtgtat
    27481 tagcctggca gtgctgggat ttttcctcgg tatagaagta cagaaacggc gactgggctc
    27541 gatgggggcg ctggccagca ccataaatat tatcgtgctg gataacccga tgactttcca
    27601 tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc ggctgtgtgc tcgcgttcac
    27661 cgttattttg cttgtgcggg ataaatcgcg cgacaggacc ggacgtgtac tgcttaatca
    27721 gtttgtttct gccgctgttt ccgcgatgac taccaatgtg gcacgtcgta aagagaacca
    27781 cctcccggca ctttatcagc agctgttttt gctgatgaat aagttcccag gggatttgcc
    27841 gaaatttcgc ctggcgctga cgatgattat cgcgcaccag cgcctgcgtg atgcgccgat
    27901 cccggttaac gaggatttat cggcgtttca ccgacaaatg cgccgcacag cagaccatgt
    27961 gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt ggccagttgc tggaagaact
    28021 ggaaatctac caggaaaagc tacgcatctg gcaagcgcca ccgcaggtga cggaaccggt
    28081 tcatcggctg gcggggatgc tccataagta tcaacatgcg ttgaccgata gttaagtcaa
    28141 aaccgacgcc aaaagcgtcg gttttttcat ggctatactt agcgatgcac ggcagaactc
    28201 gccgcgaaac gtgacggtgg caacagatga atatttatac ctttgatttt gatgagattg
    28261 agagtcagga ggatttttat cgtgacttta gccaaacctt tggtctggct aaagataagg
    28321 tacgcgatct cgactcacta tgggatgtgt taatgaacga tgtcctgccg ctaccacttg
    28381 agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg ttttggcgcg ttaatattgc
    28441 tgtttgatga ggcagaggaa gagctggaag ggcatttgcg ttttaatgtt cgtcattagc
    28501 gcaaaaaaaa gcccccgaac cgggggcaat atcgtcggac aagacgatga gggtttattt
    28561 gtacagttca gccgtagcgt gccaggtgtc accgctacga gcttcagtaa tctggtaggc
    28621 cgttgcgcct ttctcttccg cttttttgtt cagcatttca cgcatatcca ttggcgaaga
    28681 cgccacacca cttacggata cggtcccgat tgcttcacga ttttgtgctt gtgcagcatc
    28741 aatggagtcg gcagcgaatg caccgaaaga gagaacagaa agtacgctta atgcagctac
    28801 agtggttttg attttcatga tttttacctc gacataatct tttagctggg cctttgtttc
    28861 gtgacccata tcacaaaatc aagtatacac taattattga actaattaat accacgctaa
    28921 ctgtttcagt tgatactaag tgttaaaaat gtgaatttta ataacaaaaa agtataaaaa
    28981 acacggttat ttattattaa ttatagtatt ttcaattaga taatgaattt tgcataatgt
    29041 gcttttttta ccgtatgtat tcattttgtg aatgatgtgt cgcggtaaaa cgcactattc
    29101 gttaaatgta tcgcaggaga aagaagggga atgagaggga taagcaacat tttccccgac
    29161 gccagaagcg acggggcaga gattaaagct cctggtcgaa cagctctaaa attgcttcgt
    29221 acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa gatggtgtca tcgccagcga
    29281 tggtgcccag aataccttct gctttgccca gtgagtccag caggcgagca attaactgcg
    29341 ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt gtagtcgata tccagcacca
    29401 gattcttcaa tggactggag gtagttggta cacccagttc agctggcagg cagtaaacca
    29461 tttccatttt ggcattgcgt gtacgtacag caccaaactt ggtcaacatc cgcgagactt
    29521 tagactgatt aatattgtca aagccttgct cctgcaacgc ggcgacgatt tcgccctggg
    29581 agctaaattt ctcttcttta agtaatgctt taaatgcttt aactagttct tcttgcttag
    29641 ccgagcttcg cataagtcac ccgatatggt ggttgataca acattattgt gcatacagat
    29701 gaatttttat gcaaacagtc agccctgaag aaggctgaaa taatgttatg aaagagcggg
    29761 attttatcaa atttcgttat tgagaaacat gcctgcgtca cggcatgcaa attctgttta
    29821 aaagtaaatt aattgttatc aaattgatgt tgttttggct gaacggtagg gtatattgtc
    29881 accacctgtt ggaatgttgc gctaatgcat aagcgactgt taattacgta agttaggttc
    29941 ctgattacgg caattaaatg cataaacgct aaacttgcgt gactacacat tcttgagatg
    30001 tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg cagcggagca acatatctta
    30061 gtttatcaat ataataagga gtttaggatg aaagtcgcag tcctcggcgc tgctggcggt
    30121 attggccagg cgcttgcact actgttaaaa acccaactgc cttcaggttc agaactctct
    30181 ctgtatgata tcgctccagt gactcccggt gtggctgtcg atctgagcca tatccctact
    30241 gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc cggcgctgga aggcgcagat
    30301 gtcgttctta tctctgcagg tgtagcgcgt aaaccgggta tggatcgttc cgacctgttt
    30361 aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag ttgcgaaaac ctgcccgaaa
    30421 gcgtgcattg gtattatcac taacccggtt aacaccacag ttgcgattgc tgctgaagtg
    30481 ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg gcgttaccac gctggatatc
    30541 attcgttcca acacttttgt tgcggaactg aaaggcaaac agccaggcga agttgaagtg
    30601 ccggttattg gcggtcactc tggtgttacc attctgccgc tgctgtcaca ggttcctggc
    30661 gttagtttta ccgagcagga agtggctgat ctgaccaaac gtatccagaa cgcaggtact
    30721 gaagtggttg aagcgaaagc cggtggtggg tctgcaaccc tgtctatggg ccaggcagct
    30781 gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg aacaaggcgt tgtcgaatgt
    30841 gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct ctcaaccgct gctgctgggt
    30901 aaaaacggcg tggaagagcg taaatctatc ggtaccctga gcgcatttga acagagcgca
    30961 ctggaaggta tgctggatac gctgaagaaa gatatcgccc tgggcgaaga gttcgttaat
    31021 aagtaattaa ttagcgaata ataaaaaacc ggagcacaga ctccggtttt ttgttttgag
    31081 cactcgactt aattggttgc cggatattcc tgaatggtga cctgcagcgt taactgctta
    31141 tcatcacgca tcactacaac cgggatcacc gaaccagggc gaatttctgc cacctgatcc
    31201 atcgtctcca gagcagagat ggccggtttg ttatccaccg aaataatcag atcgttgacc
    31261 tgaatacccg cattcgccgc cgggccgtca ggtgacactt cattaaccac gatcccttgc
    31321 agttgatcta taccaccgcc ctgcgcgtgc agtggtgcga tctcgcgtcc gccgatacca
    31381 atgtagccgc ggatcacgcg accatcgcgg atcagcttat ccataatttt ggttgctaac
    31441 tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc catcgttact cttatcaaac
    31501 gacagcgtgt taatgcccat cagttctccc agcgagttca ccagcgcgcc gccagagtta
    31561 ccgtggttaa tggaagcatc ggtttgcagg aagttttgcc gcccggtcgg gttcagaccg
    31621 attcgacccg tagcactaat aatcccctgg gtaatggtct gcccgaggtt gtacgggtta
    31681 ccgatcgcca gtaccacgtc gccaatgtgc ggtacgcgac gtgcattgat cggaatggta
    31741 ggtaaaccgc cagtggcatt aattttaagt accgccagat cggttagaga gtcagatccc
    31801 accagcaatg cttcaaatac acgtccatcc tgtaaggcga cgatgatctg atcggcgtcg
    31861 ttgatgacgt gtttattggt gatgatataa ccgcgttgat ccatgattac accggatccc
    31921 agggtgcgga tctcaagctg gttgtgagag ttggtgttca aaccacggtt gtaaacgtta
    31981 accaccgctg gcgcggcgcg gcgaaccgcc agattataac tggcaggcgt ctcatcggta
    32041 ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca gcgaaggcat ggcaaccagc
    32101 agaatagcgc cgacaattaa tccaatcgca acggaacgta agagcttcac aaacatgatg
    32161 gaggcgtcat taaaaaaggg aacggcagca gcataccacg agttaaccgg acatcacacg
    32221 taagcctgat gcccggttta cgacattaac gcatcagcag atagatgctt tcattaccgc
    32281 gtacaatttg cagggcgatg atggccggtt ttgccgccag cactttacgc atttcagcaa
    32341 tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc gtctttttgc aagccagcct
    32401 gagcagctgg gcttcctttg acaacttcat cgatcttaat acctttgccg ccatctttta
    32461 gctgaccatc gctcaacgtt gcaccttcca gcgctggcgt gatcatttca gcgctggccg
    32521 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    32581 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    32641 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    32701 ttgccgagcc agaacctggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    32761 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    32821 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    32881 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    32941 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    33001 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    33061 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    33121 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    33181 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    33241 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    33301 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    33361 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    33421 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    33481 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    33541 cctgaaatga cgccgagaga gttaacccga cacttaacgc taatgcactc aacagctggg
    33601 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    33661 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    33721 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    33781 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcatc tgcaccggtg
    33841 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    33901 acagttccgg cagcaggctg ctggagcttt tcgccatgtg ctgatacagc tggcgatagt
    33961 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    34021 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    34081 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    34141 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    34201 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    34261 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    34321 aacggcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    34381 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    34441 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    34501 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    34561 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    34621 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    34681 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    34741 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    34801 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc gcccgcggca
    34861 ttaccctggt agcgacgtca aatattccgc cggatgaact ttatcgaaat ggcctgcaac
    34921 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    34981 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    35041 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggcgaaac
    35101 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    35161 accagacgct ggcggtctct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    35221 atattgcgct ctcacgtctc tttcatacgg tcatgttgtt tgatgtacca gttatgacgc
    35281 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    35341 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggcgatcggc
    35401 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    35461 tgaagcgcga gcatttggcg ggttaaaacc tgtcacaaat cacaaaaagg ggtcgatctt
    35521 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa
    35581 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    35641 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    35701 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    35761 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    35821 gcacaaagcg gaatacactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    35881 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    35941 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    36001 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    36061 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    36121 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    36181 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    36241 ccaacgttct ctggaacagt acttcggtcg tgaaactgct cgcatggtag ttcgtcagcc
    36301 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    36361 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    36421 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    36481 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    36541 attggcttct gctccggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    36601 atggttaaat tctgaatcag cgtaaaaatt ggaaagttgc tttttgctac cgtctgacag
    36661 acaggcaaaa caaaaaccac atcgccaata agggactaag tcaactattt cagactaaag
    36721 cgcatctctt tttccccatt tccggcatcg actcaccaca aaggtcgcaa aatctggtaa
    36781 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    36841 acaaagagag tcgttcctta ctgggtaaca caacttctga ctggccacct ggtggctggt
    36901 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    36961 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    37021 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    37081 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    37141 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    37201 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    37261 actggtacac gctgatgaac accatcatca acggttcagc ttctgaagca gatgccgcac
    37321 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    37381 tcctgagcga tgagttcagc ctggtcgatt gctatcttgc tccgctgctg tggcgtctgc
    37441 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    37501 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    37561 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    37621 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacttgg tggtggatgt
    37681 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    37741 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtac gctttaacgc
    37801 gcgctttggc ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    37861 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    37921 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    37981 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    38041 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaaacaggc cctggtggcc
    38101 tgttttgtct tttaatggct taataaaata gcgtgattgt ggcaatcata ttcaccacga
    38161 aaccgactgc catggggatc gctgttcgct tcaccaccgc aaaaggcgaa acgcctgcaa
    38221 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    38281 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    38341 gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca atggcacaaa
    38401 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    38461 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    38521 ccgcgaccac cagcgttacc acgttggcaa actgcgtacc catgccgtca aaaaacgcct
    38581 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    38641 ccgtcaaact cagtaacatc accgccacca gatgcagttc tgactgcatc agcccgatgt
    38701 gggcgaggaa cagcgagccg agcatcaaga ttaacggcat cacaggtaaa atggcgtaat
    38761 agagcggcgg gacattatcg agagctttta gctctgcctg ttcgtgattg atatttttat
    38821 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    38881 aagaggcgac cggaagctgg tagtggaaga agtaagtggc aattttcatt cccgcgacct
    38941 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    39001 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtga
    39061 ccatcagcaa catacccagg ccggaggcgc tggtaataaa ctgcgccatg atttggccga
    39121 tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac gggcggctta
    39181 gcaagctcac catcgcgcga ctggctcctg tgcgctccat gtagcgggca taaccgccca
    39241 ctgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc aggatttcat
    39301 tataaatatc gacgatcaag tagccgctgc ttttggcggg atcgagtaca ccaccgaacc
    39361 ctaaccagac accgcacatc atcaggaaga tacctgcggc agccagcacc acctgaggtt
    39421 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    39481 cgaacattgt tgaactccgt gtcaaaagaa aacggtcaat cccataaacg gcagattgaa
    39541 aacaatgatg ttatattttt tgcaagtcta tttatggtgc ggatgtcgtg tt
//
LOCUS       DAZGOB010000036        38678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGOB010000036 DAZGOB010000000
VERSION     DAZGOB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.036"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            169..2412
                     /gene="pdeF"
                     /locus_tag="ACHLIF_001916"
     CDS             169..2412
                     /gene="pdeF"
                     /locus_tag="ACHLIF_001916"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO9605945.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2451..3992)
                     /gene="ppx"
                     /locus_tag="ACHLIF_001917"
     CDS             complement(2451..3992)
                     /gene="ppx"
                     /locus_tag="ACHLIF_001917"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO9605946.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3997..6063)
                     /gene="ppk1"
                     /locus_tag="ACHLIF_001918"
     CDS             complement(3997..6063)
                     /gene="ppk1"
                     /locus_tag="ACHLIF_001918"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO9605947.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANVQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6234..6872)
                     /gene="purN"
                     /locus_tag="ACHLIF_001919"
     CDS             complement(6234..6872)
                     /gene="purN"
                     /locus_tag="ACHLIF_001919"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO9605948.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6872..7909)
                     /gene="purM"
                     /locus_tag="ACHLIF_001920"
     CDS             complement(6872..7909)
                     /gene="purM"
                     /locus_tag="ACHLIF_001920"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO9605949.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8234..8860
                     /gene="upp"
                     /locus_tag="ACHLIF_001921"
     CDS             8234..8860
                     /gene="upp"
                     /locus_tag="ACHLIF_001921"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO9605950.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8946..10235
                     /gene="uraA"
                     /locus_tag="ACHLIF_001922"
     CDS             8946..10235
                     /gene="uraA"
                     /locus_tag="ACHLIF_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO9605951.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHHSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10330..11031
                     /gene="hda"
                     /locus_tag="ACHLIF_001923"
     CDS             10330..11031
                     /gene="hda"
                     /locus_tag="ACHLIF_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO9605952.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11169..11528)
                     /gene="arsC"
                     /locus_tag="ACHLIF_001924"
     CDS             complement(11169..11528)
                     /gene="arsC"
                     /locus_tag="ACHLIF_001924"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO9605953.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11549..13012)
                     /gene="bepA"
                     /locus_tag="ACHLIF_001925"
     CDS             complement(11549..13012)
                     /gene="bepA"
                     /locus_tag="ACHLIF_001925"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO9605954.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13225..14286
                     /gene="yfgO"
                     /locus_tag="ACHLIF_001926"
     CDS             13225..14286
                     /gene="yfgO"
                     /locus_tag="ACHLIF_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO9605955.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14324..15172)
                     /gene="focB"
                     /locus_tag="ACHLIF_001927"
     CDS             complement(14324..15172)
                     /gene="focB"
                     /locus_tag="ACHLIF_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO9605956.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15194..17206)
                     /gene="hyfR"
                     /locus_tag="ACHLIF_001928"
     CDS             complement(15194..17206)
                     /gene="hyfR"
                     /locus_tag="ACHLIF_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO9605957.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17236..17649)
                     /gene="hyfJ"
                     /locus_tag="ACHLIF_001929"
     CDS             complement(17236..17649)
                     /gene="hyfJ"
                     /locus_tag="ACHLIF_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO9605958.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17642..18399)
                     /gene="hyfI"
                     /locus_tag="ACHLIF_001930"
                     /pseudo
     CDS             complement(17642..18399)
                     /gene="hyfI"
                     /locus_tag="ACHLIF_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18396..18941)
                     /gene="hyfH"
                     /locus_tag="ACHLIF_001931"
     CDS             complement(18396..18941)
                     /gene="hyfH"
                     /locus_tag="ACHLIF_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO9605959.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(18951..20618)
                     /gene="hyfG"
                     /locus_tag="ACHLIF_001932"
     CDS             complement(18951..20618)
                     /gene="hyfG"
                     /locus_tag="ACHLIF_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO9605960.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDHRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(20608..22188)
                     /gene="hyfF"
                     /locus_tag="ACHLIF_001933"
     CDS             complement(20608..22188)
                     /gene="hyfF"
                     /locus_tag="ACHLIF_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO9605961.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22193..22843)
                     /gene="hyfE"
                     /locus_tag="ACHLIF_001934"
     CDS             complement(22193..22843)
                     /gene="hyfE"
                     /locus_tag="ACHLIF_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO9605962.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22855..24294)
                     /gene="hyfD"
                     /locus_tag="ACHLIF_001935"
     CDS             complement(22855..24294)
                     /gene="hyfD"
                     /locus_tag="ACHLIF_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO9605963.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASKVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24311..25258)
                     /gene="hyfC"
                     /locus_tag="ACHLIF_001936"
     CDS             complement(24311..25258)
                     /gene="hyfC"
                     /locus_tag="ACHLIF_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO9605964.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25269..27287)
                     /gene="hyfB"
                     /locus_tag="ACHLIF_001937"
     CDS             complement(25269..27287)
                     /gene="hyfB"
                     /locus_tag="ACHLIF_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO9605965.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27287..27904)
                     /gene="hyfA"
                     /locus_tag="ACHLIF_001938"
     CDS             complement(27287..27904)
                     /gene="hyfA"
                     /locus_tag="ACHLIF_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO9605966.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28157..28627)
                     /gene="bcp"
                     /locus_tag="ACHLIF_001939"
     CDS             complement(28157..28627)
                     /gene="bcp"
                     /locus_tag="ACHLIF_001939"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO9605967.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28627..29199)
                     /gene="gcvR"
                     /locus_tag="ACHLIF_001940"
     CDS             complement(28627..29199)
                     /gene="gcvR"
                     /locus_tag="ACHLIF_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO9605968.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29345..30223
                     /gene="dapA"
                     /locus_tag="ACHLIF_001941"
     CDS             29345..30223
                     /gene="dapA"
                     /locus_tag="ACHLIF_001941"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO9605969.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30240..31274
                     /gene="bamC"
                     /locus_tag="ACHLIF_001942"
     CDS             30240..31274
                     /gene="bamC"
                     /locus_tag="ACHLIF_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO9605970.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31487..32200
                     /gene="purC"
                     /locus_tag="ACHLIF_001943"
     CDS             31487..32200
                     /gene="purC"
                     /locus_tag="ACHLIF_001943"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO9605971.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32354..32581
                     /gene="vapB"
                     /locus_tag="ACHLIF_001944"
     CDS             32354..32581
                     /gene="vapB"
                     /locus_tag="ACHLIF_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO9605972.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32581..32979
                     /locus_tag="ACHLIF_001945"
     CDS             32581..32979
                     /locus_tag="ACHLIF_001945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO9605973.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33126..33989
                     /gene="ypfJ"
                     /locus_tag="ACHLIF_001946"
     CDS             33126..33989
                     /gene="ypfJ"
                     /locus_tag="ACHLIF_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO9605974.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34004..36019
                     /gene="tmcA"
                     /locus_tag="ACHLIF_001947"
     CDS             34004..36019
                     /gene="tmcA"
                     /locus_tag="ACHLIF_001947"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO9605975.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36093..36791
                     /gene="ypfH"
                     /locus_tag="ACHLIF_001948"
     CDS             36093..36791
                     /gene="ypfH"
                     /locus_tag="ACHLIF_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9605976.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36901..37101)
                     /gene="ypfN"
                     /locus_tag="ACHLIF_001949"
     CDS             complement(36901..37101)
                     /gene="ypfN"
                     /locus_tag="ACHLIF_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO9605977.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37129..38256)
                     /gene="dapE"
                     /locus_tag="ACHLIF_001950"
     CDS             complement(37129..38256)
                     /gene="dapE"
                     /locus_tag="ACHLIF_001950"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO9605978.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAANLQLLARMYQRIMEQLVA"
     gene            complement(38260..38616)
                     /gene="yffB"
                     /locus_tag="ACHLIF_001951"
     CDS             complement(38260..38616)
                     /gene="yffB"
                     /locus_tag="ACHLIF_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO9605979.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9423 a         9947 c        10171 g         9137 t
ORIGIN      
        1 ataattcatt aatcagggtt attttgtttg atatatatca attggaactt tcatatgaca
       61 cttagaatca atgctctcta tagtgatgaa taatcatcat tcgaagtcag gtgggatgcc
      121 tgtctgaata cacctccttc aggatgtggg ggattctgct gagcatctat gaaactgaat
      181 gcaacttata taaaaatacg tgataaatgg tgggggcttc cgctgttcct gccttcttta
      241 atcttgccca ttttcgccca cattaatact ttcgcgcata tttcttccgg tgaggttttt
      301 ctcttttatc tgcctttggc actgatgatc agcatgatga tgtttttcag ctgggcggca
      361 ttgccaggga tcaccttagg gatttttgtc cgcaaatatg cagagctggg tttttacgaa
      421 acgctctcat taacggctaa ttttattatc attatcattc tctgttgggg cggttacagg
      481 gtctttactc cccggcgtaa caacgtttca catggtgata cccgtttaat ttcccagcgt
      541 atattctggc agattgtgtt tcctgcaacg ctgtttctga tacttttcca gtttgctgca
      601 tttgtaggat tactggcgag cagagaaaat ctggtcggtg tcatgccttt taacctcggg
      661 actttaatca attatcaggc cttactggtg ggtaatctga tcggtgtccc gctgtgctac
      721 ttcatcattc gggtagtgcg aaatccattt tatttacgta gctattattc gcaattaaaa
      781 cagcaggttg atgccaaagt caccaaaaaa gagttcgcgc tctggctact ggcattaggt
      841 gctttattgt tgctgttatg catgccgtta aatgaaaaaa gcacaatttt tagcaccaat
      901 tataccttgt cattattgct gcccctgatg atgtggggag cgatgcgcta tggttataag
      961 ctgatttcgc tgctctgggc ggtcgtgttg atgatcagca tccacagcta tcaaaattac
     1021 attcccattt atcctggcta taccacgcag ctgaccataa cctcctccag ttatctggta
     1081 ttctctttta ttgtcaatta tatggctgta ctggcaactc gtcagcgagc ggtagtcaga
     1141 cgcattcagc ggcttgcgta tgtggacccg gtggttcatc tgccaaatgt tcgcgccctg
     1201 aatcgcgcgt tgcgtgatgc cccctggtct gcgctttgtt atttacgcat ccctggcatg
     1261 gaaatgctgg ttaagaacta tggcatcatg ctgcggattc aatacaagca aaaactttct
     1321 cactggctgt cacccttgct ggaaccgggt gaagatgttt atcagctttc gggtaacgat
     1381 ctcgcgctgc ggctgaatac agaatcgcac caggagcgca ttaccgcact ggatagccat
     1441 ctcaagcaat ttcgtttctt ttgggatggc atgccgatgc aaccgcagat tggcgtcagt
     1501 tactgctatg tgcgctcgcc agtgaatcat atctacctgc tgctgggaga gctaaatacg
     1561 gtcgccgaac tttccatcgt gaccaacgcc ccggaaaata tgcagcgtcg cggggcaatg
     1621 tatttgcaac gcgaattgaa agataaagtc gcgatgatga atcgactaca gcaggcgctg
     1681 gaacacaacc attttttcct gatggcccag ccgattaccg gtatgcgtgg tgatgtttac
     1741 catgaaattc ttctgcgcat gaaaggtgag aatgatgaac tgatcggccc cgatagcttc
     1801 ttgccggtcg cgcacgaatt tggtttatcg tcgagtatcg acatgtgggt cattgagcat
     1861 acgctgcaat ttatggctga aaacagagcg aagatgcccg ctcaccgttt tgctattaat
     1921 ctgtctccaa cctcggtatg tcaggctcgt tttcctgttg aagtcagtca gttgctggat
     1981 aaatatcaga ttgaagcgtg gcaacttatt tttgaagtca ccgaaagtaa tgctctgacc
     2041 aatgttaagc aggcgcaaat caccttgcag catcttcagg aattaggctg ccagattgcg
     2101 attgatgatt tcggcaccgg ctacgccagc tatgcgcggc ttaaaaatgt gaatgccgat
     2161 ctgcttaaaa ttgacggcag ttttatccgc aatattgtgt caaatagtct ggattatcag
     2221 atagtggcat cgatttgcca cctggcgcga atgaagaaaa tgcgggtagt ggcagagtac
     2281 gttgaaaacg aagagatccg cgaggcggtg ctctctttgg ggatcgatta tatgcagggt
     2341 tatcttattg gtaagccgca accgttaatt gatacgctga atgaaatcga acccattcgc
     2401 gaaagtgcct gaataatgcg ggccgacatt tctcgtcggc ccgcaaagca ttaagcggcg
     2461 atttcaggtg tactttcttc ttcaattttc aaccgccagc cagccacgcc ttcccagtat
     2521 tcttgctcct tttccagatc aagcagtacc agcgcattct gactaaacca gtcatgcggg
     2581 aaacgcagtg tccagtgact gtcatcagta atcagtgtca atgttggcgg tgtggtggtt
     2641 gcctgacgtt gattgtttag taatacgcca aggcgcaata gctgtatcag tggcaggaac
     2701 tgtttcttct taaacaaggt aaagcgcggc agatcgtcga gcttaatcgc tttacggtga
     2761 tagcgcacca gtgtcgccat catcagctgc tgttcctgat taaaacccgg caagtcactg
     2821 ttttgcagaa tataagcgga gtggcgatgc aaaccgctgt ggttgatatt caacccgacc
     2881 tcatgcagca tggcggccca tcgcagtagc gcctccagtt gcggatgcgc cagcttcggt
     2941 tgctgttccc gccactgttc gtacatttgc atagtggtat ccagcactcg tcgggcctgt
     3001 tcgctgtcga tgtgatactg gttggcgagg ctgctggcgg tgcgactacg cacatcctga
     3061 tgacggaaac gtccttccat ttcatacagt acgccttcgc gtaacgcccc gtcagaaagg
     3121 cgcagttcac ggatggctaa agcatcaaac acaccacata aaatcgccag tccaggaacg
     3181 aagactgttt tccgctcttc ggaaagaccc ggtaaactca gcgatgcgaa attacggtga
     3241 cgtaaaactt cttttaccag tttttccaga cgttccgggg taattatccc gtctttctcg
     3301 cccatttcca ttaatacttc atgggcggct tttatggtac cggaagcgcc cattgccacg
     3361 ttccagccct gaatacggaa ttgccaggtt aaagtttcca gtttttgtgc tgccgccatg
     3421 cgagcgcgct gaaaattctc tttattgatg accccgccag ggaaataaag ctgggcaaag
     3481 ctgacacaac ccatccggcg gctttcaacg agaataggtt cgaaattttc accaatcacc
     3541 agttccgtag atccgccgcc aatatcaata accagtttgc gacctttttc cggttgggta
     3601 tgttccacgc ccataaaaat cagacgggct tcttcattac cggaaataat ttcaatcggg
     3661 tagggaatga ccttttccgc gcgtttcaga aagtcagtgg cgttcagcgc ctgacgcagt
     3721 gtatgggtgc caactataca gacgctggca ggagaaaacc cttgtagccg ttcggcaaac
     3781 agcgacagac agttcaaacc gcgcgtcatt gcctcttcac tcaacatatt atctggcccc
     3841 aggccgtccg ccagatgcac ccgctgtttc aggcggccaa taatctgcat ggcaccatct
     3901 accacacggg ctatgaccat gtgaaaactg tttgaaccaa gatcgaccgc agcaaactcc
     3961 tgcggacgag gggatttatc gtgtattggc atagggttat tcaggttgtt cgagtgattt
     4021 gatgtagtca taaatcgcca actgcgcccg tactttgcgg cgattgccgc ggggaacgta
     4081 gcgattactg agttctttat cgatataacg tgctttgacc gtatcgctga acaatatgtc
     4141 gatgatgtcc agcacccgct gcttcaggcg cggatcgagc agcggtgtcg ccacttcaat
     4201 acgataatca atattgcgcg tcatccagtc ggcggaagaa aggtagacct ttttatcgcc
     4261 gccattttca aaaatataaa cccggtcatg ttcaaggtaa cggtcaacaa tactgatggc
     4321 acgaatgttg tcgctaatgc cttccagatt ggggatcagc gaacacattc cgcgaaccag
     4381 cagattaacc ggtacgccgg agctggaggc cgcatacaga cgatcaacca ggcctttatc
     4441 gacaaggtta tttagcttca gggtgatacc actgggcagc ccttgctgca cgttggcgat
     4501 ttcgcggtct accatttcat ataacagacg gcgagagttt tgcggtgaca ccattaaata
     4561 atcaaatgtc actgggcggt atgggttttc aataaagtta aatacccgcc gtacttcgtt
     4621 ggtgatacgc gcatctgcgg tcagcaacga atagtcagta taaagacgcg cggttttttc
     4681 gttaaagttc ccggtcccga tgtgtgcgta acggaccact tcaccgtttt ctttacgtga
     4741 aatcaggaac agtttggcgt gaattttcag ccccggcgca gagaagataa cgtgcacgcc
     4801 tgcttcggtc aggcgcttcg cccagtgaat gttggcttct tcgtcgaaac gcgcctgtaa
     4861 ctcaaccacc acagtgactt tcttaccgtt atgtgcggcg tggatcatcg agtcgatgat
     4921 gcgtgaatct ttcgccacgc ggtaaatgtt aattttaatc gccagtacgc tcgggtcgaa
     4981 cgaagcctga cgcagcagtt ccagcacatg ctcaaaggtg tgataaggat aatagagcaa
     5041 cacatcgcgt tcgcgaatgg catcaaaacc attgcggaac tgggctttat caaaccaaat
     5101 gtggcgtaaa cgcggcagtg gtttgttcac cagattggct ttgccgacat tggggaaatt
     5161 aataaagtct ttaaaattat gataacgacc gccggggacg atggagtcgt agcgggaaat
     5221 agtcagtttt tcgcgtaaca cttcaaccag cgcattgggc atatcgcgct gataaacaaa
     5281 acgcaccggc tcagcagtta aacgctgctt gagactggaa gacatcaact ccatcaggct
     5341 ggcttccatc tcatgcacta aatcgtattc ggcatcgcgt gtcatcttca ttgaataggc
     5401 attcagcgcg tcataatcaa agaagccttt gaaaatatca tcaaggcagt aacgcagaat
     5461 gttatccaga agaatcatcg gcttgcgtcg acgcggcgct tctggcggta aattcacaaa
     5521 gcgcggcact ttatctgatg ggatctccag cagcgcgtaa cggatggtat caccacggat
     5581 aatttccacc gccagatagg tgtaatcatc cttcaggaac tgcactaagt cagtgtcagg
     5641 attgattaaa atcggcgtaa tgtgctgacg cagatactgc ttaaaataat gacgaagcca
     5701 gttttgttga ttgacggaga gctggcgttc attaatcagg aagatctggt tgcgcgccat
     5761 ctccagcaat agctcgttgt agaggccgtc gaattcctga tcggctttca gcacccggga
     5821 ctgaattttg cccagtaaat ggcgggaatg agagttggag ccttgttctt cgctaataat
     5881 gatgcgtcgc ttcagttcag cgaagcggac tttatagaac tcatcaaggt tattggaata
     5941 gatccccagg aaacgcatcc tttcaatcag cgggttagat ttgtccgccg cttcctgaag
     6001 cacgcgttca ttgaacgata accaactgag ctctttttcg atgtatagct tttcctgacc
     6061 cattaccact tttactccgt tttattcacg ggacactgcc tggatattat ggcgagcaat
     6121 attggcagat gtccaactat gccagaaaag ggggaaaaaa actgctggag cttgcgattt
     6181 acaggtgaat tgaaaaacgc cagcggcaga gctggcgctt taactacggg gcattactcg
     6241 tcggcagcgt agccctgcgg cggcagacgt tgaccatcca gccacgcggc gttttcgtgc
     6301 attttcagac gaccatcggc aaaccagcta atcaccagtg gataaatagc gtgttcctgg
     6361 gtttgcacgc gggcggtgat gtcatcttcc gtatcaccag caaataccgg gactttcgcc
     6421 tgtaaaataa ccgggccacc gtccagttca tcggtgacga aatgcaccga tgtaccgtgc
     6481 tcttcatcac cattttccag cgcttgacga tgggtgtgta atccgggata tttcggcagc
     6541 agagaagggt gaatgttcag caaacgcccg gcatagtggg agacaaacgc cgggctgaga
     6601 atgcgcataa aaccagccag cacgaccaca tcgggtgcgt acatgtcgat ttcatgaatc
     6661 aactcccggt cataggcttc acgactgtca aacgcgctgg cgatgagcgt atgcgttgca
     6721 atacccgcct ggcgggcgcg ttcaaggccg aacgcgtcgg ccttattgct gaaaactgcc
     6781 cgtacggtgc ctttaatttt gttggtttta caggcgtcaa taattgcctg taaattactt
     6841 ccgttaccgg aaataagcac cacaatattc attattcgat aaccacgcgt tgttcggaat
     6901 cagaggcttt gatgataccg attttccacg cgttttcacc gttggcattg agcagggcga
     6961 gggctttgtc cacttccgga gcaggcaggg caataatcat cccgacgccg cagttgaagg
     7021 tgcgatacat ttcatggcgc tcaacgttac ctgccgtttg cagccagttg aacacttccg
     7081 gccactgcca ggaagattca tcaatcactg cctgagtatt atctggcaat acgcgtggaa
     7141 tgttttccca gaagccgccg ccggtcaggt gcgcaatggc atgcacatcg accttttcaa
     7201 tcaactccag cactgacttc acgtaaatgc gggtcggtgc cagcagataa tcggctaatg
     7261 gcttaccatc aagttgggtg gtttgcggat cacaaccgct gacttcaaga attttgcgca
     7321 ccagcgaata gccgttcgag tgcggaccgc tggaaccgag tgcaatcagc acatcgccgt
     7381 cgctgacttt agagccgtcg atgatttctg atttttctac cacgccaacg cagaaacccg
     7441 cgacatcgta atcctcaccg tgatacatcc ccggcatttc tgccgtttcg ccacccacca
     7501 gtgaacagcc cgattgcaga catccttccg cgataccgct gatcaccgct gaagcggtat
     7561 caacatccag ttttccggtt gcgtaatagt cgaggaaaaa cagcggctct gcaccttgca
     7621 ccaccaggtc attaacgcac atggcgacca gatcaatacc aatggtgtcg tgacgtttta
     7681 agtccattgc cagacgcagc ttggtaccta cgccgtcagt gccggaaacc agcacgggtt
     7741 cacgatattt ttgcggcaat gcacacagcg caccgaagcc gcccagaccg cccatcactt
     7801 ccggacgacg cgttttcttc actacgcctt tgattcttcc aaccagagca ttacccgcgt
     7861 caatatcaac accggcatct ttgtagctaa gagaggtttt atcggtcact gcttgggtcc
     7921 ccacgcgtta cttgcggtag aaaaataaaa ttcggcgcaa ttctaacagg gaaagcaaac
     7981 gtttgcgaga ctgctttaca caaccttttt gcacgtcttt tgcccaggcg cgcggcgaaa
     8041 gaagacttgt accagggtaa aggttagttt tcggatggaa taatcttctt tcataaccat
     8101 ttgaatataa aataacttta tctcaagccg ttatcatttt gactaaagtc aacgaaaaga
     8161 atattgccgc cttgaagaaa ggaggtataa tccgtcgatt ttttttgtgg ctgcccctca
     8221 aaggagaaag agtatgaaga tcgtggaagt caaacaccca ctcgtcaaac acaagctggg
     8281 actgatgcgt gagcaagata tcagcaccaa gcgctttcgc gaactcgctt ccgaagtggg
     8341 tagcctgctg acttacgaag cgaccgccga cctcgaaacg gaaaaagtaa ctatcgaagg
     8401 ctggaacggc ccggtagaaa tcgaccagat caaaggtaag aaaattaccg ttgtgccaat
     8461 tctgcgtgcc ggtctgggga tgatggacgg tgtgctggaa aatgttccga gcgcgcgcat
     8521 cagcgttgtc ggtatgtacc gtaatgaaga aacgctggag ccggtaccgt acttccagaa
     8581 actggtttct aacatcgatg agcgtatggc gctgatcgtt gacccaatgc tggcaaccgg
     8641 tggttccgtt atcgcgacca tcgacctgct gaaaaaagcg ggctgcagca gcatcaaagt
     8701 tctggtgctg gtagctgcgc cagaaggtat cgctgcgctg gaaaaagcgc acccggacgt
     8761 cgaactgtat accgcatcga ttgatcaggg actgaacgag cacggataca ttattccggg
     8821 cctcggcgat gccggtgaca aaatctttgg tacgaaataa agaataaaaa taattaaagc
     8881 cgactttaag agtcggcttt tttttgagta aagcgcctat aacacataat acagaggata
     8941 atactatgac gcgccgtgct atcggggtga gtgaaagacc gccactttta cagacaatcc
     9001 cgcttagttt gcaacatttg ttcgccatgt ttggtgcaac cgtcctggtg cccgtcttat
     9061 ttcatattaa cccggcgact gtactgttat ttaacggtat tggaacgctg ctgtatctct
     9121 tcatctgtaa agggaaaatt ccggcttatc ttggttccag ctttgccttt atttcaccgg
     9181 tattgttact gttgccgtta gggtatgaag tcgcgctggg cggctttatt atgtgcggcg
     9241 tgctgttctg cctggtttct tttatcgtga agaaagcggg gaccggctgg ctggacgtgc
     9301 tgtttccacc tgcggcaatg ggcgcaatcg ttgccgtcat cggtctggag ctggcgggcg
     9361 tagctgccgg tatggcgggt ttactcccgg ctgaagggca aacgccagac tccaaaacca
     9421 tcatcatctc tattaccacc ctggcggtca cggttttagg ttccgtgctg tttcgtggtt
     9481 tcctggcaat tatcccgatt ttaattggcg tgctggtggg gtacgcgctc tctttcgcaa
     9541 tgggaattgt cgataccacg ccgattatta atgctcactg gtttgcgctg ccaaccctct
     9601 atacgccgcg cttcgagtgg tttgccattc tgactattct gccagcggcg ttagtggtta
     9661 ttgccgaaca cgtagggcac ctggtagtaa cggctaatat cgtcaaaaaa gatctgctgc
     9721 gcgatccagg tctgcaccat tcgatgtttg ctaatggctt gtcgaccgtg atttccggct
     9781 tctttggctc tacgccaaat accacttacg gagaaaacat tggcgtgatg gcgatcaccc
     9841 gtgtttacag tacctgggtt atcggcgggg cggcgatttt cgctatcctg ctttcctgcg
     9901 tcggtaaact ggctgccgct atccagatga tcccattgcc ggtgatgggc ggcgtttcgc
     9961 tgctgcttta tggtgtcatc ggtgcttccg gtattcgtgt tttgatcgaa tcgaaagtgg
    10021 attacaacaa agcacagaac ctgatcctga cttccgtgat tttgatcatc ggcgtcagtg
    10081 gcgcgaaggt aaacatcggc gcggcggagt taaaaggtat ggcgctggcg accatcgtcg
    10141 gtatcggctt aagtttgatc ttcaaactga tcagtgtgct gcgccctgaa gaagtggtgc
    10201 tggacgcaga agacgcggat ataacagaca aataatgtac cgaccgggca gtgttgctgc
    10261 ccggttcaaa catcatggga ttctgtggta aacttctcgc gattttgtga aatcctggtt
    10321 gaggtatctc tgaacacacc ggcacagctc tctttgccac tttatcttcc tgacgacgaa
    10381 acctttgcaa gtttctggcc gggggataac tcctctttac tggctgcgct gcaaaacgtg
    10441 ctgcgtcagg aacatagcgg ttacatctat ctctgggcac gcgaaggcgc ggggcgcagc
    10501 catctgctgc acgcggcttg cgcggaattg tcgcagcgtg gcgatgcggt gggctatgtc
    10561 ccgctggata aacgcacctg gtttgttccg gaagtgctcg acggtatgga gcatttgtcg
    10621 ctggtctgta tcgacaacat tgagtgtatt gcaggcgatg agttgtggga gatggcgatt
    10681 ttcgatctct acaatcgaat tctggaatcg ggcaaaacac gattgttgat caccggcgat
    10741 cgtccaccgc ggcagttgaa tctgggatta ccggatctcg cgtcgcgact cgactggggg
    10801 cagatctaca aattgcagcc actttctgat gaagataagt tgcaggcgct acagttacgc
    10861 gcgcgtttgc gtggttttga actgccggaa gatgtggggc gtttcttgct gaagcggctc
    10921 gacagagaaa tgcgcacgct atttatgacg ttggatcagt tggatcgtgc gtcgattacc
    10981 gcgcaacgta agctgaccat tccgtttgtg aaagaaattc tgaagttgta gataaggtgt
    11041 ttattgtcgg atgcggcgcg aacgccttat ccgaactacg cgaagttaat ccatagtatt
    11101 cgtaggccgg ataagacgtg gcaagcgtcg catccggcaa tgtgcttcaa ctagcgatgg
    11161 agcacatctt aacccacgat ttccagtacc tgctccggtg gacgaccaat ccgcgctttg
    11221 ccattcgcga ccacaatcgg gcgctccatt agcttcggat tatccaccat tgcctgaatc
    11281 agcgcctctt cgctaagcga actgtcagcc aggttcagtt ctttataaag atcctctttc
    11341 tggcgcatca attcacgggc gctattcatc cccagcattt tcagcaaatc gcgcagcgtt
    11401 gccgcatcgg cgggtgtctc aaggtagagt accacttctg gctccacgcc gttttctttc
    11461 agcaggttca gcgtttcccg gctctttgaa cagcgtgggt tatggtagat ttttacctgt
    11521 ttggtcattg ttcttccttt aatgcgaatt acatcttggt ataaggctta aagcgttcct
    11581 gcagctggcg caactggtcg atgcgcgcat cgtaacgcgc ttgttgcagg ctgcctaatt
    11641 tcacctgcga actggcgcta ctcaacagcg aaatggcctg atcgagtcgt ccggcgagcg
    11701 cataaccttc tgctcgcgca gccagctcct gatcgcggtt atttagcgcg gcttccgcct
    11761 gtgccagtaa atcccagccg ttgctgtcat ctttattatt aaaggtgtag cgattcagaa
    11821 tattggccgc ttcttgtggt tgaccgcctt gtagataagc gttcgccagg ttgagctgca
    11881 acaccggatt ggtgcgcaaa tcacgggcat ttttcaggcg attgattgcc tcattggctt
    11941 tgttttgccc aagatcgata tcggtagcca gatcgagata ccatgcgttg ccaggttctg
    12001 ccgccagtaa cggttgcagc gttttacgcg cctcgtcgta tttattggct tccatcgcct
    12061 gtaaagcacg accatattgc gccgctcgtt gctgacgaac gtttcctttc gcccattcat
    12121 ccagcaaatc actggtgagc tggttacgtc cggaattata catccccagt gtgcgcgctt
    12181 tcgccagata gaaatcttcc gacgactgca ccaccatcgg tcgcatctga ttagcacggt
    12241 tgcgggcatc tgccagacga ctttccggca acgggtgagt caataaaatt tccggcgggc
    12301 gcgaggagta acgcgcctga tcgagtaatt tttcgaggaa ggttggcatc gcctgcggat
    12361 cgaatcccga gcgttgcagc acctgaatac caatgcggtc cgcttcctgt tcattttgct
    12421 gggtgaaact gatcattccc tgacgtgttc ccgccagtgt accggtcagc gccgccatcc
    12481 ccgcctgcgg actggccatc gccagtaaaa tagaacctaa cgcgccgacc caggtcagcg
    12541 gcgcgctgcg ttgttgatct tccatcgctc gcgccaggtg acgttgggtg acgtgggaga
    12601 tttcgtgcgc cataactgaa gccagttgac tttcgttatc ggaataacgg aacagggcag
    12661 agtgcagcac cacgttgccg ccaaagaaag caaaggcgtt aatttcgtcg ttgttgatca
    12721 gaaaaaaatg aaacggtgtc ttaaccgaat tggcatgcga aaccagacgc atccccagcg
    12781 aattaatata ttgcgttaac agcgggtcat taattaacgg cgcgctgccg cgtagctggc
    12841 ggacataata gtcgcccatc tgcatttcct gaccaatgga aagcgtgctt cctgcggagg
    12901 ttcccatatc cggcaaggtg tctgcgctgt cggcaaacgc cggggctacc tgaccaatgg
    12961 tcatagcagc aatgagggtt gcaaccaggt tttttttcaa ctgcctgaac ataacctcta
    13021 tcctgtattt ctgaagatag tcatttgacc gatactgtta aatatcgttc ccaccgcgcc
    13081 gcctttgagt gtaacggcga agattagccc actcaaatga cttttcagtt tcctgaatcc
    13141 gtcataaaaa gcgaagtctt ttttgtgaca tttcgataca attgcggatc gcaatcccgc
    13201 tcttgaggtt cagggaaggt ttttatgctc gaaatgttga tgcaatggta tcgccgccgt
    13261 tttagcgacc cggaagcgat tgccttgctg gttattttag ttgccggatt tggcattatc
    13321 tttttcttta gtggcctgct tgctccgttg ctggtggcta ttgtgctggc ctatttgctg
    13381 gaatggccaa ccgtgcgcct gcaatctatt ggctgctccc gccgctgggc gacgtcgatt
    13441 gtattggtgg tttttgtcgg tatattgcta ctgatggcgt tcgtggtact gcctatcgcc
    13501 tggcaacagg gcatctactt aatccgcgat atgccgggga tgctcaataa gctttctgac
    13561 tttgccgcca cgttgccgcg ccgctatccg gcgttaatgg acgcgggcat tattgatgca
    13621 atggccgaaa atatgcgcag tcggatgctg accatgggcg attcggtggt gaaaatttcc
    13681 ctcgcctcgc tggtcggttt gctgaccatt gccgtctatc tggtgctggt gccattgatg
    13741 gtcttcttcc tgctgaaaga caaagagcag atgctgaacg ccgttcgtcg ggtgctgccg
    13801 cgtaaccgtg ggctggcagg acaggtgtgg aaggagatga atcaacaaat caccaactat
    13861 atccgcggca aagtgctgga gatgatcgtg gtagggatcg ccacctggct ggggttcttg
    13921 ctcttcgggc tgaactattc gctgctgctg gcggtgctgg tcggcttctc ggttcttatt
    13981 ccgtacattg gcgcatttgt ggtgaccatt ccggtggttg gcgtggcgct attccagttt
    14041 ggcgcaggca cggaattctg gagctgcttc gcggtgtatc tgattattca ggcgctggac
    14101 ggcaacctgc tagtaccggt gttgttctcc gaagcggtta acctgcatcc gctggtgatt
    14161 attttatcgg tggtgatctt cggtggtttg tggggattct ggggcgtatt cttcgccatt
    14221 ccattggcga cgctgatcaa agccgtgatc cacgcctggc ccgatgggca aatcgcgcaa
    14281 gaataatctt cagcccgacg aactgtttcg ccgggctgat ttttcaggat tcctgacgta
    14341 aataaatagc ccgataacac atactcacca gcactgcacc gccgataata ttacccagca
    14401 tcaccgggag cagattggcg gtaataaaat ggctgaccgt cagtgccgga aaattgtctg
    14461 cgctactgtg cgccagttgc cagaaggagg taggggcgaa atggcgaatg gcaatggcga
    14521 acggaatcac aaacaaattg gcgatacagt gctcaaagcc actggcgaca aacagggtga
    14581 tgggcaaaat catggcgacg attttgtcgc ataacgaacg cccgcaataa ctcatccaca
    14641 gcgccaggca aaccatcaga ttgcacataa tgccgaggct gacagattca gtaaatgtat
    14701 gatgcatttt gccctcggcg cagtgtaaaa ccgcgactcc ccacatcgcg ttttcactca
    14761 tcaccagccc ggaaaaccag attaacaaac tgaaacaggc aatacctgcc agattaccgc
    14821 aggccaccag aagtgcgtta atcagccaag ttcgccaact aataacgccc ctactttttg
    14881 ccattaccgt cattaccgac gaggtgaaca ggttggtgcc gcaaaccgcc agcaaaataa
    14941 agccaagtgt aaagcaaagg ccgcccacca gatgggttaa tgcctgtgaa gacggggcat
    15001 cggcgataac tgaaaggtaa aaagtaaatc cgatcgccat aaacacgcca gcggacatcg
    15061 ccattaaaaa gaccgacact ttactgcggg caattttatg ggcggcaatc cgttcagcga
    15121 ttgccgcttt tctggcgctc aactgcaagt cgaaagagag tttgtttctc atgcatactc
    15181 ctgcaaaagc agattacaac acctcgcgaa ccgagattcc cagacgctgc attcgtgaca
    15241 gcagcgtggt gcgcttcatc cctaatcgtg tcgccgcgcc acggggcccg gcaacaatgc
    15301 cattggtttc tcgcaatacc tgaacaatgc gctgacgttc ttcttcgtca ttctctggcg
    15361 tcgtcgggtg cagcaactgc gccattgaac tgcgaagcgc tgaatcttca tttagcgtcg
    15421 gcagtaaacg gctttgtcgg acattaagat gtaaattcag actgttaccg cgagtcagca
    15481 gtaccgctcg ctcaatcacg ttttccagct cgcgcacgtt gcccggccag tcccacgaca
    15541 tcaactggcg tagcgcctcg gtcgggatgg cgtcaattga acgattcata tggcgcgcca
    15601 ttttttgcgt gaaatgcttt gctaaaagag ggatatcttc cggacggtcg cgcagcggcg
    15661 gcaattccag tgggaagaca ttcaggcgat aaaagagatc gctgcgaaac tggcgatctt
    15721 caaccatttg ccacaaatca cggttggtgg cggcaatgac tctgacattt accgggatcg
    15781 ttctactccc gccgagacgc tcaatctccc gttcctgcaa tacgcgcagc agtttaggct
    15841 gaagttctaa cggcagatcg ccaatttcat cgagaaacaa cgtgccgcca tcggcaattt
    15901 caaaacgacc acgatgggta ttaatcgcac cagtaaacgc ccctttgtca tgaccgaata
    15961 actcactttc cagaagactg gcggggatgg cagcgcagtt gattttgacc agcggcttgt
    16021 cgcgtcgcgg gctaagttga tggatcgctc tggcgatcac ctctttgccg gttccggttt
    16081 caccgcaaat caacaccgta ctgtcgctct tcgccacaat atctacctgc tggagtaggt
    16141 cttccattgc ctggctttga tagataatgt caccgatgcc cagattcgaa aggagctgct
    16201 cgctaagctg gtggttttct tgctgcaaac tttcctgcaa atcggtcatg ctacgccagg
    16261 catcggcatt gccaacggca atagcgatgc gatcggctat gtgttgtagt agctggcagt
    16321 tttcctcact aaagagagtg gaagaggtat gcgccagcaa caatgcaccc ggtgtatggt
    16381 tgccaaaggt aagcggtata aggagcgcag attcgcagcc gttagatgca agtaagagta
    16441 ataacgggtc gcgttgccag agaaacagat cgtctgcacg gtgcgtcaag gtcggttgtc
    16501 gggtttgtag cgtttgtgtc agcaatacac tgtcgccagg catattgcgt ggcagacacg
    16561 cacaatgtga ggcagaaaga tcgctgcacc acaggctaaa cttctcgttc tttcgatgat
    16621 cgcccagtac catactgact gaagccagac cgaaaaaatg atggatctca cgagcgacgt
    16681 cagcgatcag atcgtcgaga tcaagatgtg acagcactgc attagtaata tcaaccagta
    16741 cccgatgatg atcgcgatct ttctccagca aacgcagcgc cacctgctgt tcttcatcat
    16801 tgagccatga ttgcagaacc ggggtgatta tccgcgccag ccagttaaac agtgtgcaat
    16861 cctggggagt aaaacggctg ccgtgggtac gaataaagcg tagctccgcc attccactac
    16921 tgccgagaag gacggcgtga cgcgaataat cgaccatctc gccctgatac tctgttgttg
    16981 ccgtcacatc gctgccatca tcatggtagc ggtaatagaa gttgcttttg agaacatgca
    17041 gttcaataag ttgtaccggg ctgaacggcg gcggcatcgg gatgaaggcg cgtaataaag
    17101 acgccttacc gtgtttctgt gctaaccgct ccgcgagcat tccgtttacc gcttcaattg
    17161 ttattccttg tggcggggca aacatcgcct cgtctgacat agccatcaac agtccttatt
    17221 aatgagaaat ccttctcacg caatttttct ggccatcaga tagatggccg gttcctgcac
    17281 aatttcatcc aacagatgaa tgaattgaat gctacgcgcc tgccacacag acgtcgcgtc
    17341 atccgccagt ggcgcgaatg cccgggtcaa cagggcggtg tggctggcgt caataacaat
    17401 ctcaccaaaa gtcatcaccc ccagcatctt acgtcgggct tgctcctctt cgacctgggc
    17461 aagccaatct tcgtattccg tcagtgggca ggggaaggcg acattcagac aatcaatcac
    17521 gccaacgtga tgaccgatag ccagcgagta atacattacc tgagagctgt gttccggcat
    17581 ctcgtcgcta ctggcgacaa acttttttcg cagcgtccag aaaacaattt ccccgcactc
    17641 ttcagtcatg taacccccgg agtacgcgaa agagttgctg aacgatacta ttgagacgtg
    17701 gatcgtcggc atcgcgcaac caggtgttaa cccgatttcc tgtaggatcg tcgcttaaat
    17761 ggcgcaggag ccgatcgcaa atttctcgcc cctgacgata gcccgccagc cgccgcgctt
    17821 ctcgctcaat ggcgatacgc tgtgatggcg ggatctgcgg ccacaacggt tgcatagtca
    17881 ccccggtggg atcgcgataa tccaccgcgt gaatcttctg ttgcagcaaa ccgagcgcca
    17941 cggcgaaacc gtgaatggtg gcggccggtg ttggcgggca gccggggatc caaacatcaa
    18001 tggggacaat ggtgtcgctg ccgccccaga cgctgtagag atcgtggaaa atccgccgcc
    18061 ggcaccgcac gcgccgtagg aaacacagat tttatgatcg ggggcagact catacgcccg
    18121 aagtgcaggc atacgcatcg cccgggtgac tgcgcctgta aataacaaaa tatcggcgtg
    18181 acgcggtgat gaaataacct taatgccaaa acgttctgcg tcgaatactg gtgtgatggc
    18241 ggcaaaaatt tcgatttcac aggcgttgca gccgccgcaa tcgacgcgat aaacgtaagc
    18301 cgagcgacgg atatcctgta gcaaatgctg cttcatcttt tgcgtttgct cgtccagcgt
    18361 gatgggctgg ctgacatgtt gtgtaagcac tggactcata gctgctcctt agccaccagc
    18421 ggtacatctg tatcgtcgtt gagcagcgtc gcgcgttgtt tgcactccgg gcagacgctc
    18481 gcttgcgccc ataacatttc gcggttttgt ggcgcatttt gttgctgtgc caacaattca
    18541 gcagccagtg cgacggtttt ttgcggggca aacgggcgtt cgcaacggct gcaacgttgt
    18601 agatggaacg tcgcgcgggt atagagatcg gctttattgg tgacggtcag ttcaaagtta
    18661 ttggtaagct ggatggctct ggtcgggcac acttcttcac aacgtccgca gtaaatacaa
    18721 cgcctcagat agagctgcca ggtgcgcgaa ttttgctggt cgtcggtctg gatagtcagc
    18781 gcatttgccg gacaagcaca ggcgcaggca ccgcaggcaa tacattggct gggcatcagg
    18841 tccggtttcc cgcgaaagcc tgggctgacc tccagtggcg cgaagggata tttcaccgtc
    18901 gcggttccgg cgcgcataat agttttcagt aacttcagca tcttctgtcc ttacttcagc
    18961 ggcgaacggt tacgatcaat accgtagcgt tcgatctctt tatacggcac ggtttttgac
    19021 tggcgcttgc gcacatctac cagcgtcaca cggtcggtac aggagtagca gggatcaagg
    19081 ctaccgataa tcagcggtgc gtcagaaacg gtattgccgc gcagcatgta acgcaacacc
    19141 ggccagttgg cgtaggtggc ggcacggcag cgccagcgga acaatttttg gttatcaccg
    19201 agcatgctcc agtgcacgtc ttcaccgcgt ggcgcttcaa caaagcccag cgcgaatgcg
    19261 tgaggtttat agctaaagcc ttcggtcagc agtggggtat ccggcatgtt gtcgagggca
    19321 aattccagca ttgccagcga atcaaacgtc tctttgacac ggaccatcac gcgggagaaa
    19381 acatcgccgc cggtaaaggt aaacagtgtt tttggaatat tgccgtagtc ggcgtagggg
    19441 tgatcaaagc gcaaatcacg ggcgatttgt cggtcgagaa tgccaatgcc ctgagtgcgt
    19501 tgttccatat tcggcgtagc aagcagcatc tctaccagct ccgacacgtc ggcgcgcatc
    19561 tcgcgcacca gtttcagcgt ttgcagacgt tgctctttga gaatatcgcg gcgaacacca
    19621 ccaatcagat tcagaccgta ggttttacgc gacccgatca gcaattccgc catcgtcatc
    19681 gacttttccc gcacgcggaa aaattgcata aagccggtat caaaaccaac gaaatggcag
    19741 gagaggccaa ggttaagcaa atgactgtgt agccgttcga cttccagcag aatcgagcga
    19801 atagtatgtg ctcgttgcgg cacctcaatc cccagtgcat tttcaaccga gttggtatag
    19861 gccacactgt gggcaaaacc gcaaatccca cacacgcggt ccgataagaa ggtcacttcg
    19921 ttgtagccca tccgcgtttc tgccagtttc tccatgccgc gatggacata aaacaggcgg
    19981 taatcagcat cgacaatttg ctcgccatcc acaaacaagc ggaagtgacc cagttcatcg
    20041 gaggtgatat gcagcgggcc gacagggatc acctgcgcat cgctgttgcc ctcattgata
    20101 aacggatacg tttcggaatc agtcgtcggt tcagggcgca gtcgataatc catcgcatct
    20161 ttgcgcagcg gatgcatatc ttccggccag tcatcgggca acaccaggcg acgatgatcc
    20221 ggcaggccaa ccggaatcag cccgtacata tcgcgaattt ctcgctcgcc ccagaccgcg
    20281 gcagggacgc gcggtgtgac tgacggaaac tcccgactgt cggcatcgac cagtgccttc
    20341 accacgatcc agcatttttc ggccccttcc attgaaaggg cataataaac cgcgtaatga
    20401 ccgttaagtg tccgctcgtc gttgccaaac aggactggaa gccagccatc atgttgataa
    20461 taaagataat gtacaacgtc aggcagcaga ttgattttca ccgtaatggt gacctgttca
    20521 ggcgtttgtc gctcttcatc cagcaccgcg ccggggaact gcgttttcag ggcggcgaga
    20581 atcgcttcgc cacgatttga cgatgaatta acgttcactg tgtttctccg aaacagatgc
    20641 ggtgcctgaa ggcaaaaaat catgccaggt gctacgttgt gcaggcaggt cgtgcgtccc
    20701 tgagaggact atagtggaag cgcccgccag gatcctgatg acaggttgtg gaatatgcgt
    20761 tcccatcgcc agcatcatga ccagcagaat caccattggc gaggtggtca accagccgag
    20821 atcaccccgg ttaacggcct gcggcggttt cgccattaac acccgcgctg ccatccgtac
    20881 caggcccgcc agcaccagcg ttaacagcaa taacagcagg acgataatca gcaggtgatt
    20941 acgtgccagt ccggcggtaa tggtcataaa ttcgctaaga aaaatgttga agggcggcat
    21001 ccctgccagc gccagcgcac cgccgccaaa cagcacggcg gtaaatggca tgattttgag
    21061 catcccacag acgacgttga gatcgcgcgt gccgtacttg agcagtacat tgccggaacc
    21121 gcagaacagc agcgtttttg ccagactgtg gtttaaggtg tgcagcagcg cggcaaaaat
    21181 tcccagtggc ccgccaatgc ctagcgccac cgcgaccagt cccatgttct ccacgctgga
    21241 gtacgccagc agacgcttaa tgtcccgctg taccagaatg aaaaatgccg ccacggcaac
    21301 cgacaacatg ccgaagatga gcaacaaccg gttggggaaa tcgctgccga tggcctggca
    21361 gataatgatg taatagcgaa ttagcaccaa cagcgcgcag ttcagcaata cggcggagag
    21421 cagggcgctg accggactcg gtgcttcact gtgagcatcc ggcagccagg cgtgcatggg
    21481 aaatagcccg gttttggtgc caaagccaat tagcacaaac acaaaggcca acagcattaa
    21541 tgttgggtca agcaaggacg attgcttaag aacctcgctc cagaatatcg ccatttctgc
    21601 ctgcggcata acgctggcga cgttggcgta taccagcacg gtaccgaaca gaccaaaagc
    21661 gacaccaaca gtacaaataa tgatgtactt ccatgctgct tccagcgatg aacgctgacc
    21721 gtaaatgcct accagaaacg ccgagcttaa ggtggtggct tcgatcgccg cccacatcac
    21781 aatcaggtta ttgctggtaa caaccagcag catggtaaac aaaaacagat ggaagaaacc
    21841 gtagtaatcg cacagcgtaa cgggggaaag ctcgccgtgt gccacttcat gacgcatgta
    21901 gccaatcgag taaacaccgg tgagaaagcc aatcacacca agaatggcga ggaacaaacc
    21961 gcccagacca tcaatatgaa gccacagtcc cgcagcgaat atttctcctg catcagcggc
    22021 agtttggacc acccagagtg ccagaatcag cagcagtgtg atccctaagc tatgtaatac
    22081 agtcaccgta caagtcgcag agagcccccg tttccggcag gcaaaacaga gcagcgaaaa
    22141 aagcagcggc gtgagcagga gtaaagcgaa catcacagaa taactcatct cattatccct
    22201 tcagcgcggt caagttgttc acgtccagcg tgccgtgggt acgccatatt tttcttgcca
    22261 gtaacaccat cacaatgacg gcgaagatgg cgtcggtagc gatacctatt tccaccagtt
    22321 ccggtgctcg ccaggcaaga agcgccagca ccagatggga gccgttttcc atcaggcagt
    22381 aaccaaaaat ttgccgcagg atattgcgct ggctgacaat gcacagcagg ccaagcagaa
    22441 aatgacctaa cgctaccgcc agcgccggtt tcagcccggt agccatcggt agcttcacgg
    22501 gctgaacgac aaatgcgcaa agcaggacaa ttaacgccgc gagcagtgcc atcattgccg
    22561 gaccgaataa cgctttttcc gggatgttct ggggaatatt tcgtgcagcg taagtcatga
    22621 ttaacggtac cagcagcact ttggtgataa aggcgctggc ggaccagatc agcagttgct
    22681 ctgcggcgaa caggcacgag agagtggcga aaatagacac cagcaccagc gactggcagg
    22741 cgtaaaatcc gcatgacagg cgatagcttt tgacgctaat cacaaacagc gatgtcagca
    22801 tcatcagtcc cgccagatta tttacgatca tagaaccggt cattcatcat ctccttactg
    22861 caaccaggtc gcggcgatta cgctggaaat cagcgacatc acaatcagta caatcaacac
    22921 caggcgcatt gaccctggca gcggtgcggc atcggcgacg gcctcgctcg gtttgccagg
    22981 cacaacgcga ccaaaccagc gaataaacca ggcgaaactg gcgaccgatt caatcatcag
    23041 cagaatcatg gcgggcagca ggatccagta ctccactgac aacgcaaaac cggcagcaaa
    23101 cagcgggaat ttactgaaga agccgttgaa cggcggcacg ccggtaatcg ccagcgctgc
    23161 cacgcagaaa cccacgcctg gcaacggcag ggtgtgcaat acgccacgca gacgcggcaa
    23221 caagcgcgtg ccgcagctgt aactcagcgc acctgctaca aggaaaaaca ggcttttagc
    23281 gaacgcgtgg ttgacgatgt aggcgatgct gccctccagc gccagccgcg agccgaagat
    23341 ggagagcgac aaaccgaaga acatccagcc aagttgagtg atggtcgacc aggccagcaa
    23401 ccgcttcata tcctgctgtg gcaaatacat cagaaagcca taaagaatgg tgaccagcgc
    23461 catgaccatg ccaacgccgc caatcacatg cgggatattg ccgccgtcga taatggcgcg
    23521 ggcaaaaatg taaacgccca ctttcacctt cgatgcggcg tggagatagg cactgatcgg
    23581 tgttggcgct tccattgcat ccggtagcca cgcttgcatc ggtagctggg ccgatttccc
    23641 ccacgcggca aacaggatgc cgccataaac cagataacgt gcgtcgccgt gtaactcgct
    23701 catcgcgcta agcgcaaacg ttccggtctg caaaaacagc gtggcggcgg caagatacaa
    23761 ccccaacgag ccgatatgag tgataagtaa cgcttttagt gctgaacgct gtgctttatc
    23821 gctctggtaa taactgatca acgcccagga gcagccgccc gtaatttcaa aaaacaacaa
    23881 ctgaccgagc agcgtcgagg agagtaccag tcccgccatc gcgccgataa acaacagcaa
    23941 aaatgcgtaa taacgattcg tgccgttatg cgggtgttcg cgatttttat ccgtcagata
    24001 acccgtggag tagatcgtga ccagcaaacc gagaaacacc accacaaaca gaatcagcgt
    24061 actcacgcga tcaatgacca ggccaaacaa tgccacatcc cccacgttga ccaatgtcaa
    24121 cgtgacggca actttatcgg cctgataaaa cgcggagatc agtgacaaca tgcacagcgt
    24181 ggtcagcgcg gcgaacaaaa ccccccattc ggcggcccga cgttgtggcg aaaacgaaac
    24241 gaccagtgcg ccgataaaag gcagcaataa cgtcgtcaga gcaagatttt ccatattccg
    24301 tcagtgctcc ttacagaccg gttaaccaga agacccatgc aagcgcagca aggctgaagc
    24361 caagccaggt cacatggtga atgagtaaaa aacgcccgcg tgccagcgtg ttttctgcga
    24421 ttgaggccag tacaaaaatc agcaaaacct tgagcagcgt aacgacaagt gaagtcagca
    24481 ggcaggcgag agaaagttct tgcgcgcgcc caaaggacag aaacagggcc acaaacagtg
    24541 atgccatcac gacctgtttc agccccagcc cccatttcgc tagcgccagc ccggcaccgg
    24601 aatattcggt cagcgggcct tcctgtaatt cctgttctgc ttcagcaaca tcaaagggaa
    24661 tttttcccat ctcaatgaag caggcaaaac cacaggccag taacgccagt acggtggtta
    24721 gcggcgagtt ccagcccatc gccagcgtat tgctgatcat ctcgatatgc gtggaacctg
    24781 ctatcagcgc caataccagc agtgagagaa taagcattgg ttcgaccaga atgccgagcg
    24841 tcaactcgcg actggcaccg actcccgcaa acggacttcc ggtatccagc ccggaaagag
    24901 caaagaaaaa acgaaacagg gcaagaagat agataagggt gatcagatcg ccgccgcccg
    24961 caaaagggga aacggtaata aacagtggta aggccatcgc cagcaccagc atgctgctga
    25021 ttaataccca cggcatcagg cggaacatca gacctgaaga tgtcggcgca acttcctggc
    25081 gtttaaacag tttgtggata tcgcgataat cctgccagat ccccggcccg cggcgggagt
    25141 gcatacgcgc gcgtatctgc cgggaaatac ccgtaaaaag tggggttagc atcagcagaa
    25201 tcacggcctg tgctaacgca aaaataacca gatatccgtc gcaaagagtt tgtctcatgg
    25261 tgatttcctt agacggcaat agcgattagc agcacaacca gtgcggcgac cacatacagg
    25321 caatagagac gaaagtcgcc gctttgcaga tgctgtattt ctttggccag ccgttgggtg
    25381 gcgctcacga tggggcggat cacccgctca tcccagaagg gttctgtgct ctgagccctg
    25441 gcggtgacgt gcgcaaaacc tttatttagc ctcagcgtag ggtcgagctg ttgtcgtaga
    25501 cgaaataacg cagagaagac cacacgcagc ggctgcatca cgccattgcc tgacggggcc
    25561 atcgcatttt cccagccgta gccgcatgcc caggcatctc ctgtgcgacg aaacgctgcg
    25621 cggcgcgaac gacaccacag ccagtaaagg ccaggcagca aaggcatcgc cagtagtaac
    25681 agcaacagta atgagggggt gacctgtgta tgaaacgtgc cgggtacaag tgctattccg
    25741 ctggcgacag tgacgggagg ggtattggta aacgcatggg caatatgcat tatcttcggt
    25801 gccagccaac tggcactaag cgcaattaat acgcagagtg ccgcgagcag tagcatggcg
    25861 acgatcatcg tatttggcac ttcctgtgcc tcttcagcgt gtgtactgcg cggcgcacca
    25921 cagaaagtaa taccgtacat ttttacgaag cacattactg ccagcccacc agtgactgcc
    25981 agcattacca tagcaatagg acccgcaagt tgtagcgcta cggcttccac acgacttagt
    26041 gagaacagcg actgccaggt gtaccattcg ctgataaaac cattcagcgg aggaatggct
    26101 gatatcgcga ggcaaccaat caggcatgct gcggctgtcc acggcatccg tttcgccagt
    26161 gcccccattt tttccatgtc gtgggtatgc aaacgcgaaa taatcgcgcc cgcgccgaga
    26221 aatagcagcc ctttgaacag cgcatggttg agcagatgaa acagtgcgcc gagcagtcca
    26281 accacggtga gcagcgggtc gtgcagtgac agaccgacca tcgccacacc gactgccagc
    26341 aaaataatgc cgacgttttc gacggtactc caggccagca gccgtttgat atcctgttcc
    26401 gccagcgcat acagcacgcc caggagcgcg gagattgcgc cgatcgccat caccagaatg
    26461 ccccaccaca gcggcaaacc cgtttgcgcc agcaggtcca tcgctacttt caggatgccg
    26521 aaaataccga ttttgaccat tacgccagac atcaacgccg aagcgtgcga tggtgcggca
    26581 gggtgagcgc gcggcaacca gctgtgcaac ggcatcatcc cggctttcgc gccaaaaccg
    26641 aaaaaggcca gcaggaacac cgccgacgcc agccccggag aaagtgaaag cgtgcggaaa
    26701 ctggcaaaat cgaggctgcc gctttcgcgc cccatcagca agaaggcgat cataatcagc
    26761 accgatccgg cgtgggcgat aaaaaagtag agcatgccag cgttgatcga cgttttatcc
    26821 tgcctggcaa tgaccagaaa ccaggaagac agcgacatca tttcaaacag cacgatgaac
    26881 caaaaagcgt tgtccatcac cagcagggca accatcgatg cgatgaaaag attcataaag
    26941 aagcccatcg ccgccgcgcc tttgccctcg tattcgcgca tataagtcaa tgaatagagc
    27001 gaacaaacca ccaccagcaa ggagataacc agcaccataa atgccgagag gctatccatt
    27061 cgcagggtaa aatcggcaaa gtcaaaaggg gtggcaaaac gggcgactaa agttacacca
    27121 gcatgtaatt gcgtaattcc gctgatgatg ccaatcacgc cgcccaccag cgatgtgatg
    27181 ccggaaagct taatagccag tctgtccaga ccgagtaaaa acagcgaagc cagactggca
    27241 aacagataga ggatcagcga ccaggttaat aattgcaggg catccattag cgttgctcct
    27301 gagtgaggga aaggggatcc tccccgccat tggcaaacca gcttgcggca aggcgctgtt
    27361 tttctttcat ctgtcgttgc aggctatcgt cggtgatcag ccgtaacgcc tgattcgggc
    27421 aagcgcgaac gcaggccgga ccttctggca agaaatcaca caggtcgcat ttcaccgcga
    27481 cggtctggac acccggttcc cagcgcagca atgcatgttg gggcggcacg ttttcttcgc
    27541 cgtctttgag tgagccttca gcctgaaaaa catattgcgc aggggcattc accggacggc
    27601 ttcctgaagc gctgattgcg ccaaatgggc agaccacggc gcaaagcttg cagccaatac
    27661 agaggctttc gttgagttgg atcgcatcat ccctctggga gatggcatta accgggcaga
    27721 cctgcaggca aggggcttcc tcacagtgat gacacacgac aggggcagtg agtgttgacg
    27781 tcttcgccag ggccaggcgc gggtgttgct gtaaaccttg cgttttatgc acgtccgaac
    27841 aggcagcgag acaggtatta catcctgtac accacagtgg ttcggccacc acaaagcggt
    27901 tcatgtcggc tccttgaaaa tgatatgaaa cattgcattt catattgcat aaaggatgcc
    27961 attctgatta actcaatata ttcaataagt tatgtattga ctggattctg aacggtacaa
    28021 aaatgatggt cattaacgtt cagcaaaaaa tggattcgac acccgtgtcg caggggaaag
    28081 cgagaatgtg atcttgatca cgagaagtga ggtatgctgg ccgcaagcgc agccagcacg
    28141 gaatggagca aagtaatcag gcgtgttctt tcagccagtt cagcacaacg tcgtggtgat
    28201 tgctggtttt gaaatcgtca aagacatgtt cgattttgcc atcagcgtca atcaggaagc
    28261 tgatgcgatg aatgccatcg taggttttgc ccatgaagga cttttcaccc cagacgccga
    28321 attgttcgca cacctggtgg tcctcatcag acaggagcgt aaagttaagc agctcttttt
    28381 ccgcaaaacg ggagagtttt tcgggtttat cggtgctgat acccagcaca tcaacgcccg
    28441 cttttttcaa ctcatccatg ttatcgcgta agccgcaggc ctgtacggta cagccggggg
    28501 tcatggcttt cgggtagaaa taaaccagaa cacgctgtcc ctggaagtcg gtcaaattaa
    28561 cttgttctcc gtcttgatcc ggcaagctaa atttcggtgc gatatcaccg gctttcagtg
    28621 gattcattac ttaactccat cctgttcatc atgttgggaa taattgacga cgttaatact
    28681 gccttgtgca ttgagttctg tacatagggc tttgaacgct tgctcaatat ttgctgcgtc
    28741 cgcagatgcg gggctgtggg cggttatctg aatatgcaac tgcgcagccc gttcattttc
    28801 agcaggttgc gtgcgcgaca ccagctccgc aatgttcata tgatgcgcgt cgaaaagtgc
    28861 tgtgaagcgt tcaattaaat gcggggagtc tgccacatcg acctgaaccc agacagatgc
    28921 tggcattggc ggacgcggac gcgccgtcgt gcgcttcatc acgattaaaa gatccagttc
    28981 ggcacctttc aacggtaacg ttgattcaat cagagtgatg gcattccatg aaccggaaag
    29041 cagcatgata aacgtgaact cttctcccag catcgccagg cgactgtctt caatattaca
    29101 gccgcaacta ctgacatgac gggtgatggt gttcacaatt ccagggcgat cggcacccaa
    29161 cgcagtgatc accagataat gttgcgatga cagtgtcaaa ctggttattc ctttaagggg
    29221 tgagttgttc ttaaggaaag cataaaaaaa acatgcatac aacaatcaga acggttctgt
    29281 ctgcttgctt ttaatgccat accaaacgta ccattgagac acttgtttgc acagaggatg
    29341 gcccatgttc acgggaagta ttgtcgcgat tgttactccg atggatgaaa aaggtaatgt
    29401 ctgtcgggct agcttgaaaa aactgattga ttatcatgtc gccagcggta cttcggcgat
    29461 cgtttctgtt ggcaccactg gcgagtccgc taccttaaat catgacgaac atgctgatgt
    29521 ggtgatgatg acgctggatc tggctgatgg gcgcattccg gtaattgccg ggaccggcgc
    29581 taacgctact gcggaagcca ttagcctgac gcagcgcttc aatgacagtg gtatcgtcgg
    29641 ctgcctgacg gtaacccctt actacaatcg tccgtcgcaa gaaggtttgt atcagcattt
    29701 caaagccatc gctgagcata ctgacctgcc gcaaattctg tataatgtgc cgtcccgtac
    29761 tggctgcgat ctgctcccgg aaacggtggg ccgtctggcg aaagtaaaaa atattatcgg
    29821 aatcaaagag gcaacaggga acttaacgcg tgtaaaccag atcaaagagc tggtttcaga
    29881 tgattttgtt ctgctgagcg gcgatgatgc gagcgcgctg gacttcatgc aattgggcgg
    29941 tcatggggtt atttccgtta cggctaacgt cgcagcgcgt gatatggccc agatgtgcaa
    30001 actggcagca gaagggcatt ttgccgaggc acgcgttatt aatcagcgtc tgatgccatt
    30061 acacaacaaa ctatttgtcg aacccaatcc aatcccggtg aaatgggcat gtaaggaact
    30121 gggtcttgtg gcgaccgata cgctgcgcct gccaatgaca ccaatcaccg acagtggtcg
    30181 tgagacggtc agagcggcgc ttaagcatgc cggtttgctg taaagtttag ggagatttga
    30241 tggcttactc tgttcaaaag tcgcgcctgg caaaggttgc gggtgtttcg cttgttttat
    30301 tactcgctgc ctgtagttct gactcacgct ataagcgtca ggtcagtggt gatgaagcct
    30361 acctggaagc ggcaccgctt gcggagcttc atgccccggc tggaatgatt ttgccggtga
    30421 cctccggtga ttatgcaatc ccggtgacca acggtagtgg tgctgtcggt aaggcgctgg
    30481 acattcgtcc accagcccag ccgctggcac tggtttctgg cgcgcgtacc cagttcacgg
    30541 gcgataccgc ttcattgctg gtggaaaatg gtcgtggcaa tactctgtgg ccgcaggtgg
    30601 ttagcgtgct gcaggcgaaa aactacacca tcacccaacg tgatgatgct ggtcagacac
    30661 tgaccaccga ttgggtacaa tggaaccgtc tggacgaaga cgagcagtat cgtggtcgtt
    30721 atcaaatctc tgttaagccg cagggttatc agcaggcggt tacggttaaa ctgctgaacc
    30781 tggaacaggc gggcaaaccg gttgcagacg cggcttccat gcagcgttac agcacggaga
    30841 tgatgaacgt tatttccgcc ggtctggata aatctgccac tgacgccgcg aacgctgcgc
    30901 aaaatcgtgc ctccaccact atggacgtac aaagtgcagc tgatgacacc ggtttaccaa
    30961 tgctggtcgt acgcgggccg ttcaatgtgg tttggcaacg tctgccagcg gcgctggaaa
    31021 aagtgggcat gaaagtgacc gacagcaccc gttcgcaggg caacatggcc gtaacttata
    31081 agccgctgtc tgacagcgac tggcaggaac tgggcgcgag cgatccaggc ctggcatccg
    31141 gtgactataa actgcaggtt ggcgatttag ataaccgcag cagcctacag ttcatcgatc
    31201 cgaaaggtca tactctgact cagagtcaga acgacgcgct ggtagctgtc ttccaggctg
    31261 cgtttagcaa gtaaaaatac agggctggaa tcatccggcc cttttttctg atatgatacg
    31321 caaacgtgtg cgtcggcagg aaaacgcgat tttagcggta attcgcacga aatttgtttg
    31381 tcggacgtag ttcggataag gcgttcacgc cgcatccgac aaaacatccg gcacaccaga
    31441 cagcaaaaga ttttaaaacg ttaattcaca cccaggagtg ataaagatgc aaaagcaagc
    31501 tgagttgtat cgtggtaaag cgaaaaccgt atacagcacg gaaaacccgg acctgttggt
    31561 gctcgaattc cgcaatgata cgtcagcagg ggatggcgcg cgcattgagc agtttgatcg
    31621 caaaggtatg gtgaacaaca agttcaatta cttcattatg agcaaactgg ctgaagcggg
    31681 tatcccgact caaatggagc gtctgctctc cgataccgaa tgtctggtga aaaagctgga
    31741 tatggtgccg gttgagtgtg tcgtgcgtaa ccgtgctgct ggctctctgg tgaaacgtct
    31801 tggaatcgaa gaaggtattg agctgaaccc gccgctgttc gatctgttcc tgaaaaacga
    31861 cgccatgcac gatccgatgg tcaacgaatc ttactgcgaa acctttggct gggtgagcaa
    31921 agagaacctg gcgcgtatga aagagctgac ctacaaagcg aacgacgtgc tgaaaaaact
    31981 gttcgatgat gctggtctga ttctggtcga cttcaagctg gagtttggcc tgtacaaagg
    32041 tgaagtggta ctgggcgatg agttctcccc ggacggcagc cgcctgtggg acaaagaaac
    32101 actggagaaa atggacaaag accgtttccg ccagagcctc ggtggcctga tcgaagccta
    32161 tgaagccgtc gcccgccgcc tgggtgtaca gctggactga tttttctggt tgcttgtttc
    32221 tttagccgga gggtactccg gctaaatgtc tttgataccc ccgctacaat acttctcatt
    32281 atctcccttt gacaggaaag tcataccagg ccaatatgta tatacataac gtatatccca
    32341 aaaagggggg gctatggaaa ctactgtatt tttaagtaat cgcagtcagg ccgtcagatt
    32401 accgaaagcg gtcgctctgc cagaagacgt taagaaagta gaaattattg cgattggtcg
    32461 tacaagaatc atcactcccg ccggtgagtc ctgggacagt tggtttgatg gcgaaaatgt
    32521 gagtgcggat tttatggata tccgtgatca acccgcaatg caggaaaggg aatcattttg
    32581 atgctgaaat tcatgctgga taccaatatc tgtattttca ctattaagaa taaaccagta
    32641 catgtcagag agcggtttaa cctgaattct ggccggatgt gtataagttc tgtaacattg
    32701 atggagctta tctatggggc agaaaaaagt cagatgccag aacgcaattt ggcggttatt
    32761 gaaggtttcg tatctcgttt ggtagtgctt gattatgata gtgctgctgc gacgcatacc
    32821 gggcaaatca gggccgagct tgcccggcag ggaaggcctg ttggaccttt tgaccagatg
    32881 atagcgggac atgctcgcag tcgcggtttg attgtggtta ccaacaatac ccgagagttt
    32941 gagcgagtgg cgggaatcag actagaagac tggagctaac attctctttt acttctaaca
    33001 ttcgtaaatt atataacgtg gcatgaagcc ttaattcctc cgccatttca tcccgctttc
    33061 ctgtggtaat cttcctctga accacctacg atcatttcta taatgaatat attgttgagg
    33121 tatctatgcg ttggcaaggg cgacgtgaaa gtgacaatgt tgaagacagg cgcaacagct
    33181 ccggtggtcc gtctatgggc ggtcccggtt ttcgcctgcc aagcggtaaa ggcgggttga
    33241 ttttactgtt agtcgtgctg gttgcaggct actatggtgt tgatttaacc gggttaatga
    33301 ctggacagcc ggtttcccaa caacaatcaa cgcggtccat tagccctaat gaagacgaag
    33361 ctgcaaaatt cacctcggtg attctggcaa ccacggaaga cacctgggga caacagttcg
    33421 agaagatggg taagacctat cagcaaccga agctggtcat gtaccgtgga atgacgcgca
    33481 ccggctgcgg ggctggtcag tccattatgg ggccgttcta ttgcccggcg gatggcacag
    33541 tttatatcga tctctccttc tatgatgaca tgaaagacaa acttggcgcg gatggcgatt
    33601 ttgcccaggg gtacgttatc gcccatgaag tcggtcatca tgtgcagaaa ctgttaggca
    33661 tcgagccgaa agttcgtcaa ctgcaacaaa acgcgacgca ggcggaagtg aaccgcttat
    33721 ctgtgcgtat ggaactccag gccgactgtt ttgccggtgt ctgggggcat agtatgcagc
    33781 agcaaggcgt tctggaaacc ggcgatctgg aagaggcgct gaacgcggcg caggccatcg
    33841 gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagatagt ttcacacatg
    33901 gcacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc gatccggcac
    33961 aatgcaatac ttttggtaaa agcatttaac ttccggggca gggatggctg aactgactgc
    34021 gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag
    34081 cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct tacctggcga
    34141 ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttcaccct cggcgctaca
    34201 aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg gctttgatgc
    34261 cgctgccttt gcagcactta gcggaacgtt gaaagcggga agctggctgg ttttgttact
    34321 ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct ggagtgattg
    34381 ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac ttacggcgga
    34441 taacgacgct atcctctggc ggcaaaacca gccgttctcg ttggcgcatt ttactccccg
    34501 tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac tcttacagca
    34561 gctactgacc atgcctccgg gcgtggcagc ggtaacggct gcgcgtgggc gcggtaagtc
    34621 ggcgttggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca ccgcgcccgc
    34681 aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct ttattgcacc
    34741 ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgacg aagccgcagc
    34801 catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt tgttaaccac
    34861 tacggtgcag ggctacgaag gcaccggacg tggttttttg ctgaaatttt gcgctcgctt
    34921 tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg gatgcccgct
    34981 ggaaaaaatg gttagtgagg cactggtttt tgacgatgaa aacttcaccc atacaccaca
    35041 aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgagc cagaaacgcc
    35101 gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acttcgccgc tggatttacg
    35161 ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa acgagattgc
    35221 cggagcgctg tggctggtgg atgagggggg attatctcaa caactcagtc aggcggtatg
    35281 ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg cgcacggtag
    35341 caatccactg gcagcgacat tgcgtggacg gcgggtcagc cggatagcag ttcatccggc
    35401 gcgtcagcgg gaaggcacag ggcagcaact tattgctggt gctttgcaat atacgcgtga
    35461 cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt tctggcaccg
    35521 ctgcggtttt gtgctggtgc ggatgggtaa tcatcgtgaa gccagcagcg gttgctatac
    35581 ggcgatggcg ctgttaccga tgagtaatgc gggtaaacaa ctggctgaac gtgagcatta
    35641 ccgtttacgt cgcgatgcgc aagctctcgc aaagtggaat ggcgaaacgc ttcctgttga
    35701 tccactaaac gatgccgtcc tttctgacga cgactggctt gaactggccg gttttgcttt
    35761 cgctcatcgt ccgctattaa catcgttagg ttgcttattg cgtctgctac aaaccagtga
    35821 actggcatta ccggcgctgc gtgggcgttt acagaaaaac gtcagcgacg cgcagttatg
    35881 taccacactt aaactttcag gccgcaagat gttactggtc cgtcagcggg aagaggccgc
    35941 gcaggcgctg ttcgcactta atgatgttcg cactgagcgt ctgcgcgatc gcataacgca
    36001 atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga gtcagcggcg
    36061 taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa
    36121 gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg gataacccgg
    36181 tggcaatggg ggaaataggt agctggtttg caccgctgtt tcctgatgcg ctggtggtga
    36241 gtgtcggtgg cgcggagccg agcggtaatc cggcggggcg tcagtggttt tcagtgcagg
    36301 gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg tttattgaaa
    36361 cggtacgcta ctggcagaaa cagagcggag taggagctaa cgccacggcg ctaattggtt
    36421 tctcacaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc cttgcttcgc
    36481 gcgtcattgc ctttaacgga cgctatgcga gcctgccgga gaccgcgtct acagccacga
    36541 caattcactt gattcatggt ggtgaagatc cggttatcga tctggcgcat gcagttgcgg
    36601 cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg gaggatttag
    36661 gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt tataccattc
    36721 cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat gatgtgattg
    36781 aaatgatgta aaagacagat tacttggcct gataagacgc gtgagcgtcg catcaggcat
    36841 cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg agcactcagc
    36901 ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa
    36961 atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttatggt cgacgcgctt
    37021 gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca gaatccacca
    37081 atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc aggcgacgag
    37141 ctgttccatg atacgttgat acatacgggc aagtagctgc aggttggcag cgttcacaca
    37201 ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct gcgcccccat
    37261 gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg gtttaatttc
    37321 attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa
    37381 tggctgcccg gaaagccacc agtccaccgt ataacgcagt tgatgttttt caagcagggc
    37441 aagcacctgt gctttgatca tctcatcagt cagttcggtg ctgaagcgga agttaaactg
    37501 cacaaacagt tcacccggaa taacgttgtt actgcccgtt cccgcctgaa tattggcaat
    37561 ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag ccactaattc
    37621 attaaggaaa ggtgctgcgc gatgtaccgg attgtcagcc agatgcgggt aggcaacatg
    37681 cccctgaacg ccatgaatgg tgaggttgca ggttaacgat ccgcgacgac catttttcac
    37741 cacatcacct accacttcga tactcgacgg ttcgccgacc aggcagtaat cgagacgctc
    37801 attacgtgcc attaacgctt cgacgacttt taccgtaccg ttgtgtgcac tggcttcttc
    37861 atcagaggtg atcagaaatg ccagtcgccc cgtatggttg ggatgttgtg cgacaaagcg
    37921 ttctgccgct accaccatcg ccgccagcga gcctttcata tctgccgcac cgcgcccgaa
    37981 taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat cggcgtcgcc
    38041 aggcggcacc acgtcggtat gcccggcaaa ggctaacgtt tcaccttgcc cacgccatgc
    38101 ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga tcgcctgcaa
    38161 acgttcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag ggcggcgaat
    38221 aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat ggaaaaattg
    38281 ctgataactg gaatcactga aacccagcag cataggctta ccaggcgcgc agagcaatgg
    38341 acgtttgata attgcaggca tttcagtcat taatgccgcc gcagaggccg catcggtgat
    38401 tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt tgagtaacgc
    38461 ttcccagcct aattcgttga taaaaccgtt caataattcg ctgtccagcc catcgacgcg
    38521 gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag cctttttaat
    38581 ggtgtcacaa tttttgatgc cgtaaagtgt aaccataatg aatccttttt cgatttatga
    38641 gaataaatac tcatttaagg gggaaaacat tttgcctg
//
LOCUS       DAZGOB010000037        38582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGOB010000037 DAZGOB010000000
VERSION     DAZGOB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.037"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(224..832)
                     /locus_tag="ACHLIF_001952"
     CDS             complement(224..832)
                     /locus_tag="ACHLIF_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO9605980.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(1258..1326)
                     /locus_tag="ACHLIF_001953"
                     /pseudo
     CDS             complement(1258..1326)
                     /locus_tag="ACHLIF_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1585..1932
                     /locus_tag="ACHLIF_001954"
     CDS             1585..1932
                     /locus_tag="ACHLIF_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605981.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(2137..2838)
                     /locus_tag="ACHLIF_001955"
     CDS             complement(2137..2838)
                     /locus_tag="ACHLIF_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9605982.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2835..3854)
                     /locus_tag="ACHLIF_001956"
     CDS             complement(2835..3854)
                     /locus_tag="ACHLIF_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9605983.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTVGKPKLDLTNTDWI
                     YGVDL"
     gene            complement(3851..4390)
                     /locus_tag="ACHLIF_001957"
     CDS             complement(3851..4390)
                     /locus_tag="ACHLIF_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO9605984.1"
                     /translation="MQPLPYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            complement(4460..4690)
                     /locus_tag="ACHLIF_001958"
     CDS             complement(4460..4690)
                     /locus_tag="ACHLIF_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9605985.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            4795..5484
                     /locus_tag="ACHLIF_001959"
     CDS             4795..5484
                     /locus_tag="ACHLIF_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO9605986.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            5566..5829
                     /locus_tag="ACHLIF_001960"
     CDS             5566..5829
                     /locus_tag="ACHLIF_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605987.1"
                     /translation="MSNKTLVKKSNNCVDAYADAFGYSSFGDGSQRLGSISFFHNVTE
                     WPVDESDDVENIKYNIATIRMPEELMLKLADFIRDQHDKAKTN"
     gene            5842..6285
                     /locus_tag="ACHLIF_001961"
     CDS             5842..6285
                     /locus_tag="ACHLIF_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9605988.1"
                     /translation="MKHDYYEAPNLNDKIAKAAESFCLHSSASSQLAGISGKRWDMLQ
                     SSGSSGSGSDNMPEKVAKLESDVAHIKRDVDELKTDVKAIDKNMITILARLDSIKESL
                     TNKPSSDAVDRKISDAKLAVLLGVPAIIAAGTGLYKLSMYFFFSA"
     gene            6836..7042
                     /locus_tag="ACHLIF_001962"
     CDS             6836..7042
                     /locus_tag="ACHLIF_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO9605989.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKRKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNALNH"
     gene            7118..7414
                     /locus_tag="ACHLIF_001963"
     CDS             7118..7414
                     /locus_tag="ACHLIF_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9605990.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            7420..8205
                     /gene="bet"
                     /locus_tag="ACHLIF_001964"
     CDS             7420..8205
                     /gene="bet"
                     /locus_tag="ACHLIF_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO9605991.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            8202..8882
                     /locus_tag="ACHLIF_001965"
     CDS             8202..8882
                     /locus_tag="ACHLIF_001965"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO9605992.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            8879..9061
                     /locus_tag="ACHLIF_001966"
     CDS             8879..9061
                     /locus_tag="ACHLIF_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO9605993.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            9034..9225
                     /locus_tag="ACHLIF_001967"
     CDS             9034..9225
                     /locus_tag="ACHLIF_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO9605994.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            9302..9517
                     /locus_tag="ACHLIF_001968"
     CDS             9302..9517
                     /locus_tag="ACHLIF_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9605995.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            9616..9834
                     /locus_tag="ACHLIF_001969"
     CDS             9616..9834
                     /locus_tag="ACHLIF_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9605996.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            9882..10160
                     /locus_tag="ACHLIF_001970"
     CDS             9882..10160
                     /locus_tag="ACHLIF_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9605997.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            10132..10197
                     /locus_tag="ACHLIF_001971"
                     /pseudo
     CDS             10132..10197
                     /locus_tag="ACHLIF_001971"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            10359..11522
                     /gene="intD"
                     /locus_tag="ACHLIF_001972"
     CDS             10359..11522
                     /gene="intD"
                     /locus_tag="ACHLIF_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO9605998.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(11538..11614)
                     /locus_tag="ACHLIF_001973"
     tRNA            complement(11538..11614)
                     /locus_tag="ACHLIF_001973"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11578..11580),aa:Arg,seq:tct)
     gene            11857..12489
                     /gene="fimZ"
                     /locus_tag="ACHLIF_001974"
     CDS             11857..12489
                     /gene="fimZ"
                     /locus_tag="ACHLIF_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO9605999.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(12492..13007)
                     /gene="sfmF"
                     /locus_tag="ACHLIF_001975"
     CDS             complement(12492..13007)
                     /gene="sfmF"
                     /locus_tag="ACHLIF_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO9606000.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(13018..13995)
                     /gene="fimH"
                     /locus_tag="ACHLIF_001976"
     CDS             complement(13018..13995)
                     /gene="fimH"
                     /locus_tag="ACHLIF_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO9606001.1"
                     /translation="MACLCLANISWATVCANSTGVAEDEHYDLSNIFNSTNNQPGQIV
                     VLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLHDYLLGAMSLVD
                     SVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMVEIPRQVMFTV
                     YVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASLFSAAGPGNRP
                     AGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITP
                     NDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
     gene            complement(14037..16646)
                     /gene="sfmD"
                     /locus_tag="ACHLIF_001977"
     CDS             complement(14037..16646)
                     /gene="sfmD"
                     /locus_tag="ACHLIF_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO9606002.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPSAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(16677..17369)
                     /gene="fimC"
                     /locus_tag="ACHLIF_001978"
     CDS             complement(16677..17369)
                     /gene="fimC"
                     /locus_tag="ACHLIF_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO9606003.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(17589..18131)
                     /gene="fimA"
                     /locus_tag="ACHLIF_001979"
     CDS             complement(17589..18131)
                     /gene="fimA"
                     /locus_tag="ACHLIF_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO9606004.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            18612..19478
                     /gene="folD"
                     /locus_tag="ACHLIF_001980"
     CDS             18612..19478
                     /gene="folD"
                     /locus_tag="ACHLIF_001980"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO9606005.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            19480..19692
                     /gene="ybcJ"
                     /locus_tag="ACHLIF_001981"
     CDS             19480..19692
                     /gene="ybcJ"
                     /locus_tag="ACHLIF_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO9606006.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVEVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            19800..20321
                     /gene="ybcI"
                     /locus_tag="ACHLIF_001982"
     CDS             19800..20321
                     /gene="ybcI"
                     /locus_tag="ACHLIF_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9606007.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(20357..21742)
                     /gene="cysS"
                     /locus_tag="ACHLIF_001983"
     CDS             complement(20357..21742)
                     /gene="cysS"
                     /locus_tag="ACHLIF_001983"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO9606008.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYIALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            21916..22410
                     /gene="ppiB"
                     /locus_tag="ACHLIF_001984"
     CDS             21916..22410
                     /gene="ppiB"
                     /locus_tag="ACHLIF_001984"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO9606009.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            22413..23135
                     /gene="lpxH"
                     /locus_tag="ACHLIF_001985"
     CDS             22413..23135
                     /gene="lpxH"
                     /locus_tag="ACHLIF_001985"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO9606010.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            23253..23762
                     /gene="purE"
                     /locus_tag="ACHLIF_001986"
     CDS             23253..23762
                     /gene="purE"
                     /locus_tag="ACHLIF_001986"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO9606011.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            23759..24826
                     /gene="purK"
                     /locus_tag="ACHLIF_001987"
     CDS             23759..24826
                     /gene="purK"
                     /locus_tag="ACHLIF_001987"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO9606012.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(24963..25856)
                     /gene="ybcF"
                     /locus_tag="ACHLIF_001988"
     CDS             complement(24963..25856)
                     /gene="ybcF"
                     /locus_tag="ACHLIF_001988"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO9606013.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFTKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(25853..26668)
                     /gene="ylbF"
                     /locus_tag="ACHLIF_001989"
     CDS             complement(25853..26668)
                     /gene="ylbF"
                     /locus_tag="ACHLIF_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO9606014.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(26679..27938)
                     /gene="ylbE"
                     /locus_tag="ACHLIF_001990"
     CDS             complement(26679..27938)
                     /gene="ylbE"
                     /locus_tag="ACHLIF_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO9606015.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(27948..29615)
                     /gene="fdrA"
                     /locus_tag="ACHLIF_001991"
     CDS             complement(27948..29615)
                     /gene="fdrA"
                     /locus_tag="ACHLIF_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO9606016.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            29931..30980
                     /gene="allD"
                     /locus_tag="ACHLIF_001992"
     CDS             29931..30980
                     /gene="allD"
                     /locus_tag="ACHLIF_001992"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO9606017.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            31002..32237
                     /gene="allC"
                     /locus_tag="ACHLIF_001993"
     CDS             31002..32237
                     /gene="allC"
                     /locus_tag="ACHLIF_001993"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO9606018.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            32248..33033
                     /gene="allE"
                     /locus_tag="ACHLIF_001994"
     CDS             32248..33033
                     /gene="allE"
                     /locus_tag="ACHLIF_001994"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO9606019.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(33162..34307)
                     /gene="glxK"
                     /locus_tag="ACHLIF_001995"
     CDS             complement(33162..34307)
                     /gene="glxK"
                     /locus_tag="ACHLIF_001995"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO9606020.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(34329..35629)
                     /gene="ybbY"
                     /locus_tag="ACHLIF_001996"
                     /pseudo
     CDS             complement(34329..35629)
                     /gene="ybbY"
                     /locus_tag="ACHLIF_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(35686..37047)
                     /gene="allB"
                     /locus_tag="ACHLIF_001997"
     CDS             complement(35686..37047)
                     /gene="allB"
                     /locus_tag="ACHLIF_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO9606021.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(37107..38561)
                     /gene="ybbW"
                     /locus_tag="ACHLIF_001998"
     CDS             complement(37107..38561)
                     /gene="ybbW"
                     /locus_tag="ACHLIF_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO9606022.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
BASE COUNT        10028 a         9862 c         9314 g         9378 t
ORIGIN      
        1 tggatatcat tttcggtatt tctctgcaaa ttccagccca caggatctcg gtgtacaatc
       61 tgtcgctgat cgcgcaaatt tagggtcaat cacttcattt tttgcatcaa accctcacta
      121 acatcgaacg attatagtac aagtagtgtt cttgacttct cattaaaccg tcagcattca
      181 attaattaca aaatactagc ggcatttctc tcccgcaact ttattacagc cgttcagaac
      241 gtaaatgcgt caatactcga ctccacttat catcctgcca cggccggaat tttacatgtg
      301 ccgtttctct ggcaaggatt gcctgtgcct tattgagtat ccggggatat tcttgttcga
      361 ttgaagtgaa gcgaccggct tcgcggtgtt ctgctacctg aagaagcgga gtgacgtttt
      421 ggcaagcggt taacattgtg tcacttgccc gccataacca agcaagtgtg caaagttcgt
      481 tatcagtgaa ttgttttgtg attggggatt gttgaatttc tcgatcgaga atatccagca
      541 cccagcggcg gaattctttg gccacaggag tgcgggcgaa catggcgatc agatgggcgc
      601 cacggaggga gaaaatgcga gtcttcttac gataatttcc tgaggtcgtt gattcgacga
      661 cctgagacat tccgtcagta aactcatcaa tatttttgtt gtagatgtcg gttactgctt
      721 tggtgctggc atattttaat gctgccgcca attctttgct ggtaaaccag atctggttgt
      781 tgtgatttat aggagtgaat tttacatcgc ggaatgcgag ctgagtgacc atagcgataa
      841 tctcgttttg aatttgagct atcaccaccg acgacgccaa tcgactggtg gtggactgtg
      901 caaggttggc gtaaccggtc aaaacgaaat ccggcgcttc cgaagaagcc ctcacacagc
      961 ccaccataat ttttgtgtag acgtgcactg cgcataaaaa aaccgcgaac gcggttatgc
     1021 gtcgttttga tgtccgggac gccaatcccg tgtgccgatt ttgcggcaac gcacagaata
     1081 tagcgccgga taaataatgt cgtcaaccct tgcatatggg gggcgcgatg atcactacta
     1141 ccaccataat cgctccaact gttgcaacta ctttaggcta agacatcacg atttccatat
     1201 cacctttagc cgccagacaa acagagactc cctgttcttg gctgccaaaa acaccgaatg
     1261 caaaaaatcg caggtgtatt tctgcatctg tggagggaga agagagtttg gattgtatgt
     1321 gcgcataaac gtccccgttt agcgcagaag tcaccgtagt tgttcatatt ctgatgacat
     1381 gataatttcc tattgaatag aaaaaattaa cacgactcat tcttatgact ggaaaaagca
     1441 atcatgctaa taatcattgt tgttccagtt ccaaatgcag cgaaataatc accaatattt
     1501 tttctaccgg tttcgacttt gatgctatat tctgctaaca cctaactaac actacgtttc
     1561 acacagcaat tacatggaaa taacatgact atcaaagcga ctgacataga gattcttcat
     1621 cgttatgctg aaggggtaat ggaacgttct aaccatcatg caaaaaatgt tggtgcagcc
     1681 gctcttacat tgctaggagg tgttatctgg aaagccctgc ccggatcaat cgagataaga
     1741 acgtataacg gaagtcttgc aaatatggtg tggtggcaaa gcgaaagaac attaaaaaat
     1801 tatgcaatct catacaacca taattcatgt gaaattgaaa tgagagatga atcagtcaaa
     1861 ggtgctgtat tatttagcat ttccaacgaa accactccag aaaaaatatt atcacagctt
     1921 tctgagcttt aattaaaggc ggtgctaaca ccgcccatac cattactcca catcttacgc
     1981 aacttgataa ttatgctcta ttaaataccc acaatatttc atcaatgcat gggtcagcta
     2041 caaaatgttt accgtggtaa ttcctgtttt cgcaacatct caaaagaaca ataaacactg
     2101 tatttcttca agtaacgaag aatctttctt tgcccgtcat acacttgctc ctttcagccc
     2161 aaacttagct ttgatttctg cgatcttcgc cagagcctgt gcacgattta gaggtctacc
     2221 gcccataaca ggaagttgtt ttactggttc aggtatcgtc tcaccacggt taattcgcgc
     2281 tgtcatacag gtcagttcat cggcagcctt gcgccgtaat tccgcgtcag tcagcgcatt
     2341 ggcccgcatg ttctggtaca ggttggtaac caaccagtaa tgcgcgttcg atttccacgg
     2401 ataagactct gcatccggat acaggccacg cttccggcaa tactcgtaaa ccatatcaac
     2461 cagctcgctg acgtttggca gcccggcgtt aacggatgct tcttcccggc accatgcaac
     2521 aaactgcccg ggtgatggca gaaatggtct attctgccga cgggcaacac gcattccggc
     2581 gctcacctgt tccatcgtgg tgatcccgtt ttcccggaaa gccagaaccc actggcggcg
     2641 gatttcgttc agttcgttct ggtcacgatt agccaggctc gccgggaaag ttgccagcaa
     2701 ctggctgaat acaccgttga ttatctgcgc tacctgctgt acctgcggct tttcgtcgta
     2761 ctgttccggc atgttgttgg cgatccgacg catctgctca cggtcaaagt taatcatctg
     2821 tgcggcgatg tttttcatag atccaccccg taaatccagt ctgtgtttgt caggtcgagt
     2881 tttggtttgc cgactgtcac gccagcctgt tgcttgttcc ggttgatttc gagctgggtc
     2941 cacttgtcgc ggagtttggc cggactcagc acgttaccgg accagaagtt gtcctggcag
     3001 gcccagcgga acagtacaca catgtcgcgg tggttacgtc catcacgttc acgcatcaga
     3061 cggatatcgt tagcccaccc tgcaaaattc ggttttctgg ctgatggcgc gatggtcttc
     3121 accatgtcaa acatccactc tgcggcggtc aggtcttctg ctgtccccca cttgctgccg
     3181 ctctgaatcg cagcatccgc tttcaccaca ggaaggtcgt tttctggcag gtcagaggat
     3241 tcgccagaat tctcggacga ataaggtttt atattgtctt ttgttagttt gtcttttgtg
     3301 tttacctgat tcgggtaagt gcctttacct gatttgggta aacttttctt acctgattca
     3361 ggtaaattta cctctttcag gtaaacttta tttttcttac ctgattcggg taatgttgac
     3421 cattcactga ccacattatt aatgccgata ttccgcccgc tctgaataaa aatcccacgc
     3481 tttaccagaa cactttttgc agcagaacac ttgtgcggca atatcccggt caactcggaa
     3541 agttgctcgt tgctcaccca gtccagtttt ttattaaagc catatgtttt gcgcatgaca
     3601 gccaggaaga ccagaagctg gtgctgtgtt aatccggcca gcattacagc ttccagcaac
     3661 tcatttgcaa tgcgcgtata accatcatcg agatctgcca cgcgcggctc cttttgtgcc
     3721 gcatccggca ctggaaaatt gaatatctca gcagtgtttg ccataattcc tcccgcaatg
     3781 agtgtgttac gatttgcacc tgaaagtcgg ttctgttcca gcagaccggc tttcgccatt
     3841 tctgaacctg tcatatcgcc cccagcatgg tagtaaccat cgccatcaat ggaccagcca
     3901 gctctgggtc cacacgaaac atcgacacaa taccttcact aatttccttc agtttctggt
     3961 ggcgtggtgc gttgagaatg acagcctgtt ttgcctcact gagttccttt tccatttcag
     4021 ccaacctagc catgaagcta tcctgctcaa ccaggtaacc gcgatattcc agcggtagta
     4081 ccgccagaat tgccggggtc agttcacgca cgttatttcg gtatttttca gaatcgaatt
     4141 tgttatcgag gaagcggaac agcttctggc gtgtacggct gacatcatca gggaaatcga
     4201 tggtgccgcc gccctgctcc cgatactcat tcacaatgag tgtggcaacg acatcctgat
     4261 tatctacagc cgaccaggcg cggacggcat cacggatttt ttcgtggcct ggcacctgtt
     4321 ttgtttgaga acgatttatc accgcagtcg ggctaaatcc gctagtctgt tggtatggaa
     4381 gtggttgcat aattgactcc tttagtttga attgactgtt aagttgattg cttattgtta
     4441 aagagcgtga aatggaaatt taagctgcgt tcttttcggt gtgtggaaac aacttcggaa
     4501 gatccgggcg aatctggtat gccttcacta ctccaccagt agccgtaaca atgctgccga
     4561 catgttcagg ggataccttt gctttgttgt gaagccactt atagacggcc tgctgtgaaa
     4621 cttcgcaggc agcgcccagt ttcttttgtg aaccaacgat attgatcgct gttttgatag
     4681 ctgggttcat aacaacctcc gtggttaatt tgaatcaaga ttaaaactat ggttgttttt
     4741 agtcaacaac cattttcgtt tgatggaata aaaccttggt tgtacatttg gactatgaaa
     4801 acaacactct cagaaagact taaagaagcc agattagcgc gaggccttac acaaaaggcg
     4861 cttggggatt tggtcggggt tagccaggct gctattcaga aaatcgaaac agggaaagct
     4921 aatcaaacaa ctaaaatcgt ggagatcgcg aacgctttgg gtgtgcgcgc agaatggtta
     4981 tcttctggcg ttggaaatat gtcagacagt acagtgcaac caatacaatc aactgtcagc
     5041 cattccaaat acttcaagat tgacgttctt gatatagaag tcagtgctgg gccgggagtc
     5101 atcaaccgtg agtttgtaga agttctacgc tcggttgagt actcgtttga cgatgctcgt
     5161 cacatgttcg atggtaggaa ggcagaaaat atccgcatca ttaacgtgcg tggtgacagc
     5221 atgtcaggaa cgatcgaacc aggtgatctg ctgttcgttg atatcacggt taaatctttc
     5281 gacggtgatg gtatctatgc atttctgtac gacgacaccg cccatgtaaa gcgcctgcaa
     5341 atgatgaagg ataaactgct ggttatctct gataacaaga gctactcacc gtgggaccct
     5401 atcgagaaag acgagatgaa ccgggtattc atattcggta aggttattgg gagcatgccg
     5461 cagacgtaca gaaaacacgg ataatcagcc gcgtgttgat gaggcttttg ggtaatacgc
     5521 tgaagaaaat accttataaa aaataacatt acgggaaagg caaaaatgag taataaaaca
     5581 cttgttaaaa aatcaaataa ctgtgtcgat gcatacgccg acgcgtttgg ctactcttcg
     5641 tttggtgacg gctctcagcg cctaggttca atttcttttt ttcataatgt aactgaatgg
     5701 cctgttgatg aaagtgacga tgttgagaac ataaagtaca acattgcaac cattagaatg
     5761 cccgaagaat tgatgcttaa attggctgac ttcattcgcg accagcatga caaagctaaa
     5821 acaaactaat cgtaattaat tatgaaacac gattattacg aagctccaaa cctaaatgat
     5881 aaaattgcaa aggcggcaga atcattttgt ttgcactcca gtgcgtcttc ccagctagca
     5941 ggtatatctg gaaaaagatg ggacatgcta caatcaagtg gtagcagtgg gtctgggagt
     6001 gataacatgc ctgaaaaagt tgcaaaactg gagtccgatg ttgctcatat caaacgagac
     6061 gttgatgaac ttaaaacaga cgttaaagct atcgacaaaa atatgataac tattcttgca
     6121 cgtctggact ctataaaaga atccttaaca aacaagccat caagtgatgc tgtcgataga
     6181 aagatttcag acgcaaagct tgcggtattg cttggtgttc cagcaatcat cgctgcagga
     6241 acaggacttt ataagctatc aatgtacttt ttctttagtg cttaagaaaa tgctcgcacc
     6301 cttgcattag gtaaagcgat ttttcattcc ccattaccct cgctcttaac caatcatcta
     6361 aacccggcca ccgtgccggg ttttcttttg ccctcccctc atcatacacc gttcaaaaaa
     6421 accaccacga cctcgcttca gttatcgcta tgcgatgcaa gtcacaaaat aaatccatct
     6481 taaatacaac cagttatatc taaaacaacc aacaaaacaa cttttgttgt tgacgataaa
     6541 acaactatag ttttaaatga gttcatcgca acaacacaac gatacggcaa tcacctgatt
     6601 caccgttgcg atgaccgctt agatccgcag cttgaatttc agcaggcttc ggggagtgcg
     6661 agggatgaaa cggacgcgtg aacgtcggtg tgaccagctg aaatcaactc aacatttcat
     6721 accttagtcg cttcaacgag gcggcttagt tatgacaacc ggcggccatc caccgcctga
     6781 atacgcgcag aagtctttat atgttcagca gcccagctta cgggcaggag tttttatggt
     6841 tcatcaacat tacggaacgc agaccgttaa tcgcggtgcg gtcatgccag gaatgctggt
     6901 caaacgcaaa gatggtacct ggactgcatc agctaattta cgcggacgac tttatctgca
     6961 tcgcggcatt gagcgcactt atacccgtga cttgctcgtg gaagtttttc tcgacggacg
     7021 cggcaacgct ctgaatcact aatccccttt cctgttttcc gaatcagcct ggcattccgc
     7081 gggcgatttt ttcacagcca ttttcaggag ttcagccatg aacgcttatt acattcagga
     7141 tcgtcttgag gctcagagct gggcgcgtca ctaccagcag atcgcccgtg aagagaaaga
     7201 ggcagaactg gcagacgaca tggaaaaagg cctgccccag cacctgtttg aatcgctatg
     7261 catcgatcat ttgcaacgcc acggggccag caaaaaagcc attacccgtg cgtttgatga
     7321 cgatgttgag tttcaggagc gcatggcaga acacatccgg tacatggttg aaaccattgc
     7381 tcaccaccag gttgatattg attcagaggt ataaaacgga tgagtacagc actcgcaacg
     7441 ctggcaggga agctggctga acgtgtcggc atggattctg tcgacccaca ggaactgatc
     7501 accactcttc gccagacggc atttaaaggt gatgccagcg atgcgcagtt catcgcattg
     7561 ttgatcgtcg ccaaccagta cggccttaat ccgtggacga aagaaattta cgccttccct
     7621 gataagcaga acggcattgt tccggtggtg ggcgttgatg gctggtcccg catcatcaat
     7681 gaaaaccagc agtttgatgg catggacttt gagcaggaca atgaatcctg tacatgccgg
     7741 atttaccgca aggaccgtaa tcatccgatc tgcgttaccg aatggatgga tgaatgccgc
     7801 cgcgaaccat tcaaaacccg cgaaggcaga gaaatcacgg ggccgtggca gtcgcatccc
     7861 aaacggatgt tacggcataa agccatgatt cagtgtgccc gtctggcctt cggatttgct
     7921 ggtatctatg acaaggatga agccgagcgc attgtcgaaa atactgcata cactgcagaa
     7981 cgtcagccag aacgcgacat cactccggtt aacgatgaaa ccatgcagga gattaacact
     8041 ctgctgatcg ccctggataa aacatgggat gacgacttat tgccgctctg ttcccagata
     8101 tttcgccgcg acattcgcgc atcgtcagaa ctgacacagg ccgaagcagt gaaagctctt
     8161 ggattcctga aacagaaagc cactgagcag aaggtggcag catgataccg gacattatcc
     8221 tgcagcgtac cgggatcgac gtgagagctg tcgaacaggg ggatgatgca tggcacaaat
     8281 tacggctcgg cgtcatcacc gcttcagaag ttcacaacgt gatagcaaag ccccgctcag
     8341 gaaagaagtg gcctgacatg aaaatgtcct acttccacac cctgctggct gaggtttgca
     8401 ccggtgtggc tccggaagtt aatgctaagg cgctggcctg gggaaaacag tacgagaacg
     8461 acgccagaac cctgtttgaa ttcacttccg gcgtgaatat tactgaatcc ccgatcatct
     8521 atcgcgacga aagtatgcgc accgcctgct ctcccgatgg tttatgcagt gacggcaacg
     8581 gccttgaact gaaatgcccg tttacctccc gggatttcat gaaattccgg ctcggtggtt
     8641 tcgaggcaat aaaatcggct tacatggccc aggtgcagta cagcatgtgg gtgacgcgaa
     8701 aagatgcctg gtactttgcc aactatgacc cgcgcatgaa gcgtgaaggc ctgcattatg
     8761 tcgtgattga gcggaatgaa aagtacatgg cgagttttga cgagatggtg ccggagttca
     8821 tcgaaaaaat ggacgaggca ctggctgaaa ttggttttgt atttggggag caatggcgat
     8881 gacgcatcct cacgataata tccgggtagg cgcgatcact ttcgtctact ccgttacaaa
     8941 gcgaggctgg gtatttcccg gcctttctgt tatcagaaat ccactgaaag cacagcggct
     9001 ggctgagaag ataaataata aacgggaggc ggtatgcaca aagcatctcc tgttgagtta
     9061 agaacgagta ttgagatggc acatagcctt gctcaaattg gagtcaggtt tgtgccaata
     9121 ccagtagaaa cagacgaaga atttcatacg ttagccacat ccctttcaca aaagctggaa
     9181 atgatggtgg cgaaagcaga agcagatgag agagaccagg tatgacaacc actgaatgca
     9241 tttttctggc agcgggcttc atattctgtg tgcttatgct tgccgacatg gggcttgttc
     9301 aatgacacct cagcaagaaa acgcccttcg cagcattgcc cgtcaggcta attctgaaat
     9361 caaaaaagcc agacagcatt ttccggataa aaacgtcgat gacatttgcc gtagcgtact
     9421 aaagaagcac cgcgaaacgg taacgctgat gggattcaca ccgactcatt taagcctggc
     9481 gatcggcatg ttaaacggcg tctttaagga acggtgaaca tgaaaagcaa aatcatcagg
     9541 gagctacagg ctcctttttt attgttcgca ttcatcctca agcgtattaa ccaacaattc
     9601 agggattaat ggaagatggc agacatcatt gattcagcat cagaaatcga agaattacag
     9661 cgcaatacag caataaaaat gcgtcgtcag aactaccaga ctgtatccgc aactcattgt
     9721 tgtgagtgtg gcgatccgat agatgagcga agacgcctgg ctgttcaggg ttgtcggact
     9781 tgtgcaagtt gccaggagga gatcgaactt aagaacaaac aatggggact gtgatggcct
     9841 caaagcagca aatttcaaca tcgtccaact gaggtgtaaa aatgttcaga atcatttttc
     9901 ctaacacctg gtacgtcgac caccacggca ctccctgcaa aatcctgcgt tctacccaca
     9961 acaaagttca ctacatccga aaaggcagaa catgtatcgc cagcatgttc cgctttaatc
    10021 atgactttga acctgtgaat aaagctgatg cagatcggat agcagaagag atcgaaacgg
    10081 cagaacacat taagaagtta cgtgacatgc gttcaaaaag cagaggtaac catggaatca
    10141 tacagcctca cactcgatga ggcctgtcag tttcttaaga tatccagacc aaccgccacc
    10201 aactggatac gaacaggccg cctacaggca acacgtaaag atccaaccaa gccaaaatct
    10261 ccttacctca caacacggca agcctgcatt gcggcgcttc agtctccgct gcatactgtc
    10321 caggtgagcg cgggtgatgg cataacagag gaaagaaaat gtcactcttc cgcagaaatg
    10381 aaatatggta tgcctcgtat tcgctcccgg gcgggaaacg aattaaggaa tctcttggca
    10441 caaaggacaa gcggcaagct caggagttgc acgacaagcg aaaagcagaa ctctggcgag
    10501 tagaaaagct aggggattta cctgatgtca cttttgaaga ggcctgccta agatggcttg
    10561 aggaaaaagc tgataaaaaa tctctcgatt cagataaaag ccggattgag ttctggcttg
    10621 aacattttga gggtataagg cttaaagata tctcggaggc aaagatttac tctgctgtaa
    10681 gcagaatgca taacagaaag acgaaagaaa tatggaaaca gaaagttcag gccgccatca
    10741 ggaaaggtaa agaaccgcct gtttatgaac caaagccagt atcaactcag acaaaggcaa
    10801 agcatcttgc catgataaag gccattctcc gtgctgcaga acgcgactgg aagtggctgg
    10861 aaaaagcgcc tgtcatcaag ataccagcgg tcagaaacaa gcgagtcaga tggctggaaa
    10921 aggaggaagc aaaacgcctt attgatgagt gccccgaacc actgaaatct gtcgtcaagt
    10981 ttgcgctggc aactggtctg agaaagtcga acatcataaa tctggaatgg caacaaatcg
    11041 acatgcagcg acgagttgcc tgggtgaatc cagaagagag caaatcaaac cgcgccattg
    11101 gtgtggcgct gaacgatacc gcctgtaaag tgttgcgtga tcaaataggc aagcatcaca
    11161 aatgggtgtt tgtacatacc aaggcggcta agcgagcaga tggaacatca acgcctgcgg
    11221 tcaggaagat gcgcatcgac agcaagacat catggctatc agcttgtcgt cgtgcaggaa
    11281 ttgaagattt ccgtttccat gacctcagac acacctgggc aagctggctg attcagtcag
    11341 gcgtcccatt atcagtgctt caggaaatgg gcggatggga gtccatagaa atggttcgta
    11401 ggtatgctca ccttgcgcct aatcatttga cagagcatgc gaggaaaata gacgacattt
    11461 ttggtgataa tgtcccaaat atgtcccact ctggaattat ggaggatata aagaaggcgt
    11521 aactgattga attgtaatgg cgcgccctgc aggattcgaa cctgcggccc acgacttaga
    11581 aggtcgttgc tctatccaac tgagctaagg gcgcgttgat accgcaatgc ggtgtaatcg
    11641 cgtgaattat acggtcaacc cttgctgagt caatggcttt tgatctggtt gctgaacaag
    11701 tgaacgaccg cgtctgattt tctgatttat ttcgctatag cggcaaacaa acacacaccg
    11761 ctgcgcgtct gaatcaagaa aacccgtatt ttcatgtatc aaagtacaat ttaccgccct
    11821 aacggaaaat tgtccgctcc tatgagactg gtaactatga aaccaacgtc ggtgatcatt
    11881 atggatactc atcctatcat cagaatgtct attgaagttc tgttgcaaaa aaacagtgaa
    11941 ttgcagattg tcctgaaaac ggatgattac cgcataacca tcgattatct ccgaacccgt
    12001 cctgttgatt taatcattat ggatatagac ttgcccggaa cagacggttt taccttcctg
    12061 aaaaggatca aacaaatcca gagcacagtg aaagtgttat ttttatcatc gaaatcagaa
    12121 tgcttttatg ctggcagagc gatacaagct ggtgctaacg gttttgtcag taaatgcaat
    12181 gatcagaatg atatttttca tgccgttcag atgatcctct ccggatacac gttttttccc
    12241 agcgaaacgc ttaactatat aaaaagcaat aaatgtagta cgaatagttc aacggtcact
    12301 gtgctatcta atcgtgaagt gaccatatta cgttatctgg ttagcggatt atctaataaa
    12361 gaaattgccg ataagttatt acttagcaat aaaacagtta gtgcgcataa atctaatatt
    12421 tatggcaagc taggtttgca ttcaattgta gagcttatcg actacgccaa attatacgaa
    12481 ttaatataat attaattata attgatcata aatatcgcat ccgctttcgc cacaccaggc
    12541 cgaacaccgc tggctaacgc tcgataattg gcaaaaaagt ttagtgtgac attgccattt
    12601 gcatctactt cctcagtcgg gctcgcctcc cccagtgcga gccgggagcg atcgctatta
    12661 cgtaattcga tggcgacggt ttgtgccatt gcgggatcat ccagagccag caggttggta
    12721 tcggatgccg gcgttcccgt aaataaaatc gcaactgaac ccggaggaca tccctccagc
    12781 cgcaggctaa aagggacgag tgccgtggta tcgccagcgt tcagtaattg tgtcgtaggc
    12841 catctgccta aatctaccgt cttatcaata tccgctgtgt ttacggtaca ggagaaatca
    12901 acaacgttac cgtgcaaatt gatattaatc gttcctaaag ggtcagctgc ccatccaatg
    12961 gaactccaca gtaacccgca gaaacagcta aagagtactc ttctcattct ccgtacctca
    13021 ttgataatcg acgcgtaaat accccagcgc gctaaacggc ccctcggtcg gtttttgacc
    13081 ggtaatactg ataggccagg cgcgaagtgt gacattggct gccgcagctg catccagacg
    13141 gaaaggaata acgctattga gatcgttagg cgtaatcggc gtatcgttct gatcggcgac
    13201 aataaaacct aaatcctgat tgtccgacac catcgcctga ccagaaacgg cactggcttc
    13261 cagacgcatt gttaaataag cctgcgcagc aacattcgta catttgaccg caatgctctt
    13321 ggtttgcggc atgacgccag ctggtcgatt ccccggccct gccgcactaa ataacgatgc
    13381 gccgatatca ccaaaatcaa attcaacaat ctgcccggca tttaattcgc agttttgcgg
    13441 cacttcaacc cggccaccaa aactaatggt ataaacaggt gtaaccaacg gatcgtaagg
    13501 cgttgatgtc acatacacgg taaacatcac ctgtctgggg atctccacca tgttaataaa
    13561 gggacgaata acctttaaac gaaatattaa acgagaatcc atcaccccga atggaaggtt
    13621 ttgcgaaacg ttaggatctg ttcccatccg aatataattt tgcgggggga actgaatatc
    13681 catcacacta tcaaccagac tcatcgcacc taatagataa tcatgtaatt gcatatattt
    13741 ataattatcg atagtttcct gaacaataaa gttggtgaca taactacggt atgtataatt
    13801 caccagcgta ccgggtggac aaattgctga gacacctacc cagccggatt tttccggtaa
    13861 aacaacaatc tgccctggct ggttattggt gctattaaag atatttgaga gatcatagtg
    13921 ttcatcttct gctacgccag tactatttgc acaaacagta gcccaggata tgtttgccag
    13981 acacaaacat gccatcgcca ataataatct gcagattatc ttattgataa tttcctttat
    14041 gagccaggat gtgtgcaaac tgccgaaata acagtgacgg cttgctgtaa gctttgcttc
    14101 ggcaatacat agtgcgcaat gcagcgtgag ttcgcgccgt ctccccactg aaccagtaat
    14161 tcaccagaca atggcgcacc acttaaataa acttgcccgt catcacccac catactggtt
    14221 attccggtac tgttttcccg taccagtgat ccaaacggca ccggttttct gccctgggta
    14281 acggtaatga gcgcccgcac gcctatgcgg gtatcaaaat tggcacgaac caacgcgcct
    14341 tgcgtcggca caacgctgct aatatttttt tcaacatcga tggaattccc catcgtattg
    14401 gtatcaagcg cgatacggtt ataccgataa accgtggcat acggcatcac cgcatagccg
    14461 cgccagtcgg ttaaaatgcc agtttgattt tcaatgcgta caccgcctgc gccaggcgct
    14521 ttaatcaaaa cattggtatc ccctaaaggc tggctcagcg ttatgccatt ttcatgcccg
    14581 accacaccgc cagacagctg ccagttaacg tcatgttgat cgcggtcgta gttatacccg
    14641 acccccagcg taccgtaagc ggcctgccag tttgccgtgg cgctgcccgt atacccatta
    14701 ttcgaggtat caccctggct tacgtgataa ctcaggttgt ggccttccag taaggtccca
    14761 cctacacctg ccagccagct attttgcccg ttgctgttac ggttggcgtt aaaggaggca
    14821 taagcgcggt cgagcgcatt ttcccgggtg tagcgacgtt tggtcagaac attgaaaggc
    14881 actgaaacat taagtccgac aatacgttcg ttatcgggga tccctacaga ttcattccac
    14941 gaaaatgaga gtgaataact gatgccaacc cagctgctgg tataccccac ctgataccag
    15001 gtatctgaat ccgaagtatt ccagtatttt tgatgggtac cagagatata aagcgagcca
    15061 aagtcattaa gtgattgtga aatatttaac tgcaaacggt ctttacggct aaagcgtaaa
    15121 ttgtggtaat tcacgattat cggttcatcg cgatgctcac cgtcgtaatc gtaatcatat
    15181 tcgtacccct ccattcgacg atacgcaaca tcatcaaggg tataaaaacc ttgtgtcgaa
    15241 tagcggtaac ccattaactg aaaattggtg ccgaaggtgt tcatcgattt cgcatagagg
    15301 aagcgaatgg aatccccctc atgacgactg tcgtcggcta actgactgcg cgcatgggtc
    15361 acatccagcg acactgtgcc ccagttcccg aggttgcgcc ccagccctaa taaaaaggcg
    15421 gtgtaattgg cagataattg cgtcccgccg taggcagtaa attcctgtgg taaaccgccg
    15481 agtgccgtac cctgaaaaaa gaaaggcgat gattgctgac tattaccgct acgaaaatcg
    15541 cccgccgtca ggtcaaattt gaaacgccct tcgcgttgta aaattggcac tgttgaatac
    15601 ggaattgtgt aattctgctg attaccatcg cgctcgtcga tggtgacgtc cagatcgcca
    15661 tttgaagatg tcgggtgcaa atctgtaatt tcaaaagcgc tgggggaaac atagctttga
    15721 tagataataa aaccattttg ccgaatcgtt agctgggccg ccgtacgggc aatcccacgt
    15781 actgttgggg caaacccttg ctggctatca ggatacatat tatcggaaga gtaaagccgt
    15841 ataccacgaa atccaacacc atcgaaaata tcacttcctg tattgccgtc tcccattacc
    15901 agttcacttt tcagcggaat aatggcgcgc tgtacccagg tgccaatatt attccactgt
    15961 tctgaatgat atccatttcc gcgaaaatag ttccaggaac cattgttgcg taaacgccac
    16021 gggccaatat taatcccgct gagctcacta aaaaaatagc tatcgttacc attacctctg
    16081 ttaccggtga aattataatt caggagtaaa gcaggaatac cttcatccca ctcttcaggc
    16141 ggaatgtaac cgtgagcgct actcaacaac gctatctgag gaatagtgat gttcaggcgc
    16201 atcgctgcaa aatcaaagtt aattgtggca tcaggtacag ctttcagtaa attgatgcat
    16261 ttattgtttt gctgctgggc taactcggga aatgcactgc tattaaggct aattcgttca
    16321 aggagtacct ggttaaaaca gggcattaac ccacctgatt tatcacctgt attttctgtt
    16381 gtcgattcaa atacgatatc ctgtgaacca atgaactcat cattacgcca gagatctacc
    16441 cgatacacgc ccgcaggttg atgattacct ctctcaaagc gcgataaatc agcaacggat
    16501 gtgccattct ctaataaaaa tgccgggttg aaataacttt ccgcatagct taaaaaggag
    16561 ggtaaaatgg caagagatga ataacaaatt ccggccagac accaggtata cctctgcgga
    16621 atatccgtag tagtgggtat tttcataatt agctctgata ttgtcagatg attcggttag
    16681 tttaagttca cttctctgac cggggttact gaaccataat cattaacggt ctgaaatttc
    16741 agctttccct gcactcctga gggtaaggga atttgtgaat taactctggg tgcagccata
    16801 gcattcccca acttttggct gcccacttgt aagttaacca gagtgacata aaaaggtgag
    16861 ggattatgaa cattgatata gttaccggat cgcgaaaact tgagtgtgtc cggtgcttct
    16921 gcgggtaatt cttgtaattg aattagacgg agaaataatt tcatgcgcga taaaatcgcc
    16981 agttgcaaaa cattcctgcc gttcaatgca tttttatcta ccgaagggat cgtcttaaca
    17041 ttcatccaga acagagactc acgatctgct gccagcggtg gaccggtgta aataatacgc
    17101 aaggtatttt cgcttttggg ttcactaaca aacagtggcg gtgtaatgat gaatgacttt
    17161 tcttttacac cgctgctgtt ttcaatccac gaattgacca gaaagcgctc attggtatgg
    17221 ctatttctaa tccataccgc agtctgttta gcatcagcgg gataaataat acgcgtggca
    17281 cctaatgcga tccctccggc agcatgggcg ctggccgaaa tgattaaata aaatataatg
    17341 agcatcaata actttatttt agtcatcata gttaatacgc cttgtgtatt cctgacggca
    17401 aaaataagaa atataaagaa atgattgctt tggtttctta caacacatca ttcccttttc
    17461 atttaataaa gcggcatgat acaaagtgaa catgatttcg atttacattg ccaccatacc
    17521 gcttcagaca accggtacat tactttgtta taagacgtga tatataattc aaaacaacgt
    17581 ggttttgatt attcgtatct cataataaaa gtcgcgtcag catttgcttg ccctgctgat
    17641 gcacttgtac cggtgccttt ataacgtgca gaaaaatgaa gaacgttggt cccggggatc
    17701 aagttctggt tggttgagaa gctattccca tcaggtttga gaattgcgga tgtattatca
    17761 agtatttcaa taccgacacc cgttgctgtt gttgtattgg tactggatgc aatggccaat
    17821 aaattatcat tgattgcatc agcctgacca gaaaatgcaa cggcagcatt agcggcaaca
    17881 acaggatcgc agtcgttcaa ctgaatggtg aatggaatta atgcagaggt attaccaaca
    17941 gcattgaaaa tatcggtacg atattgaccg agtgtgacaa cctggtctgc cgagtcagta
    18001 ttcactgaac aggcagcatt caccagttcg ccttcaaagt gaattgtacc gccatctaca
    18061 acggcagcat aactacccgt agcggcgaat agtgcggcag ccagcgcaga cgaaataaat
    18121 cttaatttca tatatattcc ttcaatctca tttatcgact ccacatccgt atataaccga
    18181 ttactttatt taagacactg atagtagtaa aattcctttt tatcctctaa gaatgtctta
    18241 attgaaaata tgcactctat tctaaaaaat agagagcccc gttagatgaa tactttcgcg
    18301 caaaatatat tcaacacaaa tatagacctg aagcggtaaa ttaccaggct gaaaattctt
    18361 tttatattgt caggtatttc ttaaattatc ttaatcctta aacaaggaaa taaatcagtt
    18421 ccagatttac aacgccatca tggacgaaaa atgaagcttt caatctcagc gacctcagcg
    18481 acggtgcgcc tcaccttcgc aagaggtcgc ttcacgcgat aaatctgaaa cgaaacctga
    18541 cagcgcgccc cgcttctgac aaaataggcg catccccttc gacctacgta acagatggaa
    18601 tcctctctct gatggcagca aagattattg acggtaaaac gattgcgcag caggtgcgct
    18661 ctgaagttgc tcaaaaagtt caggcgcgta ttgcagccgg actgcgggca ccaggactgg
    18721 ccgttgtgct ggtgggtagt aaccctgcat cgcaaattta tgtcgcaagc aaacgcaagg
    18781 cttgtgaaga agtcgggttc gtctcccgct cttatgacct cccggaaacc accagcgaag
    18841 cggagctgtt ggagcttatc gatgcgctga atgccgacaa caccatcgat ggcattctgg
    18901 ttcaactgcc gttaccggcg ggtattgata acgtcaaagt gctggaacgt attcatccgg
    18961 acaaagacgt ggacggtttc catccttata acgtcggtcg tctgtgccag cgcgcgccgc
    19021 gtctgcgtcc ctgcaccccg cgcggtatcg tcacgctgct tgagcgttac aacattgata
    19081 ccttcggcct caacgccgtg gtgattggcg catcgaatat cgtcggtcgt ccaatgagca
    19141 tggaactgtt gctcgcaggt tgcaccacta ccgtgactca ccgtttcact aaaaatctgc
    19201 gtcaccacgt agaaaatgcc gatctgttga tcgttgccgt cggcaagccg ggctttattc
    19261 ctggtgactg gatcaaagaa ggcgcaattg tgattgatgt cggcatcaac cgtctggaaa
    19321 atggcaaagt tgtgggcgac gtcgtgtttg aagacgcggc taaacgcgcc tcatacatta
    19381 cgcctgttcc gggcggcgtt ggcccgatga cggttgccac gctgattgaa aacacgctac
    19441 aggcgtgcgt tgaatatcat gatccacagg atgagtaaca tggcgacatt ttctttaggt
    19501 aaacatccgc acgttgagct gtgcgacttg ctgaaactgg aaggctggag cgaaagcggc
    19561 gcgcaggcga aaatcgcgat tgccgaaggc caggtggaag tcgacggtgc ggttgaaacg
    19621 cgcaaacgct gcaaaatcgt cgccggtcag acagtgagtt ttgcaggtca cagcgtacag
    19681 gttgttgcct gatccctttc ccggcctgta tcaacaggcc ggttctcttt tcagactttt
    19741 gccctctacg ccccgttcat ccatgctgat agcatcgaca ttttgccgaa gcgaccatca
    19801 tgccaaccgt tattacgcac gccgccgttc ccctatgcat tggtttaggg ttgggttcga
    19861 aagtcatccc tccgcgtctg ttatttgccg gaatcatcct ggcgatgctg cccgacgccg
    19921 acgtattgtc gtttaaattt ggcgttgctt acggcaatgt ttttggtcat cgcgagttta
    19981 cccattcgct ggtgtttgcg tttgttgtcc cgctgttatg tgtgctgatt ggacgccgat
    20041 ggttcagggc tgggctgatt cgctgctggc tgtttttaac cgtctcgttg ctatcgcaca
    20101 gcttgctgga ttcggtaacc actggcggta agggcgttgg ctggctgtgg ccgtggtcag
    20161 atgaacgctt tttcgctccc tggcaggtga ttaaagtcgc gccgtttgca ttgtctcgtt
    20221 acaccacgcc gtacgggcat caggtgatta tttctgaatt gatgtgggtg tggttgccgg
    20281 ggatggtgct tatgggaatg ttgtggtggc gcaggcgata accggatgcg aaaactcgca
    20341 tccggcaaca gcgcaattac ttacgacgcc aggtggtccc ttgcgggcca tcttccagca
    20401 cgatccccat ctcgttaaga cggtcacgcg ccgcatctgc cgccgcccag tctttcgctt
    20461 tacgggcatc cagacgctgt tgaattaacg cttcaatctc agccacttcg ctgtcgtctg
    20521 cctgcgcacc gctttgcagg aacgcttccg gttcttgctc cagcaggccc aatacagcgg
    20581 aaagtttacg caggtgagat gccattgcat tcgccgctgc catatcttct gctttcagac
    20641 ggttcacttc acgcgccata tcaaacagca cggaataggc ttccggggtg ttgaaatcgt
    20701 cgtccatcgc ttcaataaag cgcgcttcaa acgcttcgcc accggcaggc gcaacggttt
    20761 tatctgtgcc gcgcagcgca atgtagaggc gctccagcgc cgcacgcgcc tgcttcaggt
    20821 tctcttcgct gtagttcagt tggctgcgat agtggcccga catcaggaag taacgcacgg
    20881 tttccgcgtc gtagtatttc agcacatcgc gcacggtaaa gaagttaccc agcgatttgg
    20941 acatcttctc gcggtcaacc atcaccatcc ccgagtgcat ccagtagttc acatactggc
    21001 catcatgggc acaggtggac tgcgcgattt cgttctcgtg gtgcgggaac atcaggtctg
    21061 aaccgccgcc gtggatatca aagtggttac ccagctgctt gcagttcatt gccgaacatt
    21121 caatgtgcca gcctggacgg cccgcgcccc acggagacgg ccagctcggt tcgccctctt
    21181 tcgacatctt ccacagaacg aagtccatcg ggttgcgttt gtcgtcgacc acgtcaacgc
    21241 gcgcacctgc ctgcagctgg tcgagatcct gacgcgacag cacgccatat gtcggatcgg
    21301 tcgggacgtc gaacatcacg tcgccgttgt ccgccacata agcgtgacct ttggcgatca
    21361 gttgttcagt gagttcaata atttctgcga tatggtgcgt cgcgcgcggc tccatatccg
    21421 ggcgcagaat gttcaaagca tcaaaatctt tgtgcatttc ggcgatcatg cgatccacca
    21481 gcgccacaaa gctttcgcca ttttcattgg cgcgtttgat gattttgtcg tcgatatcgg
    21541 taatgttgcg cacatacttc agtttatagc cgaggaaacg cagatagcgc gcaaccacgt
    21601 caaaagcaac aaaggtacgc ccgtgaccga tatgacagag atcgtaaacg gtgattccac
    21661 acacatacat gccgacttcc ccggcgtgaa taggcttaaa ttcctctttt tggcgtgtca
    21721 gagtattgaa gatttttagc atcgaagatt ccgtttagac atgtgtgggt aattgagttg
    21781 cgtataatac ccatatttcc cgcgcgaatc agcatacatt gcgtgatgat ccgatcgtgc
    21841 ggttatgcta taacaccacc ctatatatga cccgaactgg gttgaagcac caatcaaacg
    21901 gaacaggatg caaaaatggt tactttccac accaatcacg gcgatattgt catcaaaact
    21961 tttgacgata aagcacctga aacagttaaa aacttcctgg actactgccg cgaaggtttt
    22021 tacaacaaca ccattttcca ccgtgttatc aacggcttta tgattcaggg cggcggtttt
    22081 gaaccgggca tgaaacaaaa agccaccaaa gaaccgatca aaaacgaagc caacaacggt
    22141 ctgaaaaata cccgtggtac gctggcaatg gcccgtactc aggctccgca ctctgcaact
    22201 gcacagttct tcatcaacgt ggttgataac gacttcctga acttctctgg cgaaagcctg
    22261 caaggttggg gctactgcgt gtttgctgaa gtggttgaag gcatggacgt ggtagacaaa
    22321 atcaaaggtg ttgcaaccgg tcgtagcggt atgcaccagg acgtgccaaa agaagacgtt
    22381 atcattgaaa gcgtgaccgt tagcgagtaa tcgtggcgac actctttatt gcagatcttc
    22441 atctctgcgt ggaagaaccg gcgatcaccg ccggttttct gcgtttttta gcgggggaag
    22501 cccgcaaggc cgacgcgctg tatattcttg gcgatctgtt tgaagcatgg attggcgacg
    22561 acgatcccaa cccactccat cgccagatgg cggcagcgat caaagcggtg tccgattccg
    22621 gcgttccctg ttatttcatt catggcaacc gtgattttct gctcggcaaa cgctttgccc
    22681 gtgaaagcgg catgacatta ttgccggaag aaaaggtgct cgaactttat ggtcgccggg
    22741 tgttgattat gcatggcgac acgctgtgca ccgatgacgc gggttatcag gcttttcgcg
    22801 ccaaagtcca caaaccctgg ctgcaaatgc tattcctcgc cctgccgttg tttgtgcgca
    22861 aacgcattgc cgcgcgaatg cgagcgaaca gtaaagaagc caacagcagt aaatcgctgg
    22921 cgatcatgga cgttaaccaa aacgcggtag tcagtgcgat ggaaaaacat caggtgcaat
    22981 ggctgatcca cggacatacc catcgcccgg cggtacatga acttatcgcc aaccagcaac
    23041 ctgcttttcg cgtggtactc ggtgcctggc atacggaagg ttcgatggtg aaagtcacgg
    23101 cggatgacgt tgagctgatt cattttccgt tttaaaaaac ccgcaacttt gctgatttca
    23161 cagccacgca accgttttcc ttgctctctt tccgtgctat tctctgtgcc ctctaaagcc
    23221 gagagttgtg caccacagga gttttaagac gcatgtcttc ccgcaataat ccggcgcgtg
    23281 tcgccatcgt gatggggtcc aaaagcgact gggctaccat gcagttcgcc gccgaaatct
    23341 tcgaaatcct gaatgtcccg caccacgttg aagtggtttc tgctcaccgc acccccgata
    23401 agctgttcag cttcgccgaa agcgccgaag agaacggtta tcaggtgatt attgcgggcg
    23461 caggcggcgc agcgcacctg cctggtatga ttgccgccaa aacgctggtg ccagtgctgg
    23521 gcgtgccagt acagagcgcc gcactgagcg gtgtcgatag cctctactcc atcgtacaaa
    23581 tgccgcgcgg cattccggtg ggtacgctgg cgattggtaa agctggcgcg gcaaacgcgg
    23641 cgttactggc agcacaaatt cttgcgactc atgataaaga actgcaccag cgtctgaatg
    23701 actggcgcaa agcccagacc gacgaagtgc tggaaaaccc ggacccgcga ggtgcggcat
    23761 gaaacaggtt tgcgtcctcg gtaacgggca gttaggccgt atgctgcgtc aggcaggtga
    23821 accgttaggc attgctgtct ggccggtcgg gctggacgct gaaccggcgg cggtgccttt
    23881 tcaacaaagc gtgattaccg ctgagatcga acgctggccg gaaaccgcat taacccgcga
    23941 gctggcgcgt catccggcct ttgtgaaccg cgatgtgttc ccgattattg ccgaccgtct
    24001 gactcagaag cagcttttcg ataagctcca cctgccgacc gcaccgtggc agttacttgc
    24061 cgatcgcagc gagtggcctg cggtgtttga gcgtttaggt gaactggcga ttgttaagcg
    24121 tcgcactggt ggctatgacg gtcgcggtca atggcgttta cgtgccaatg aaaccgaaca
    24181 gttaccggca gagtgttacg gcgaatgtat tgtcgagcag ggcattaact tctctggtga
    24241 agtgtcgctg gttggcgcac gcggatttga tggcagcacc gtgttttatc cgctgacgca
    24301 taacctgcat caggacggta ttttgcgcac cagcgtcgct tttccgcagg ccaatgcgca
    24361 gcagcaagcg caagccgaag agatgctgtc ggcgattatg caggagctgg gctatgtggg
    24421 cgtgatggcg atggagtgtt ttgtcacccc gcaaggtctg ctgatcaacg aactggcacc
    24481 gcgtgtgcat aacagcggtc actggacaca aaacggtgcc agcatcagcc agtttgagct
    24541 gcatctgcgg gcgattaccg atctgccgtt accgcaaccg gtagtgaata gtccgtcggt
    24601 gatgatcaac ctgattggta gcgatgtgaa ttatgactgg ctgaaactgc cgctggtgca
    24661 tctgcactgg tacgacaaag aagtccgtcc ggggcgtaaa gtggggcatc tgaatttgac
    24721 cgacagcgac acatcgcgtc tgaccgcgac gctggaagcc ttgatcccgc tgctgccgcc
    24781 ggagtatgcc agcggcgtga tgtgggcgca gagtaagttc agttaagcag gtgctcattt
    24841 gattgccgga tgcggcgtaa acgccttatc cggcttaccg ctccggtacc cattgtaggc
    24901 ctgataagac gcgtcaagcg tcgcatcagg catggtgcac cgattgccgg atgctctcat
    24961 cactacagcg aaatacaggt ccccgcttcg cccgccagcg tctcttcaat tcgcgataac
    25021 gccccaatcc acgcgggttt accacggctt ctgacataac cacttaccgc cgttaccttc
    25081 ggccccatcg aaccatcggc tttggtaaat ggcgctaact catccggtgt ggcatggcga
    25141 atggcgcgtt gctgcggcgt tccccagttt tcataaaccg cgtcagcatc ggtgaggatc
    25201 accagtccat cggcattaat ctgctcggcg agcaacgcag cggcgagatc tttatcaatc
    25261 accgcttcac tccctgcccc atcttccgtc acaggcacac cgccgccgcc actgcaaatc
    25321 accacatgcc cctctttgag caacaactcg atggcttcgc tgtcgagaat tttacgcggt
    25381 tgcggagacg ccaccacccg gcgcaaatat ttaccatcac gtttcatctg ccagccgtaa
    25441 gccgcttcca gtgccttttg ttcttctggc tgataaaccg gaccaataaa tttctctggc
    25501 tgcaaaaacg ccggatcatc aggcgaaacc tcaatgcgcg tcagcaccgt cgtgacaggc
    25561 ggcatctgcg gctgtgcgct caaactctgc gccagcatat agccaatcat cccctggctt
    25621 tccgccacca gcacatccag cggatacgga tctacctctt tccacgccag attctgcaat
    25681 gccagcagcc ccacctgcgg cccattgccg tgaacaatcg ccagccgata agaacgggcc
    25741 aggcgcgcca gcgcgggtac agcactggcg atattgcgat attgattttc tgccgtcagc
    25801 gcctcaccgc gctggagtaa ggcgttgccc ccaagagcta caaccagtgt tttcatggtt
    25861 ttccttgtaa taattgttgg ccaagccaga accccagcag cgtatctgcc cctgacgtat
    25921 gccccagcgc cagcagcgaa tcaatcgcaa cagcggtacg cttcgggcaa ctcagagcat
    25981 ggacaaagtg caggagtggc gaggcgaaat atccttgcgc ggcataacgt aaataactga
    26041 cgctcaccgc tgtggtaacg agttgaagat tgtcggaaca ggcaaaaaac gggcgaccgg
    26101 cgcgcgcatc taaagcgcca taataccagg ccgccagcag cattccgctc aacgtgtcat
    26161 catgacttgg cgttaatccg gggcctttac ccagccagtg ccgccagtcg gtcttaacgc
    26221 cattgagcgc ggcctgaaaa cagtgacgaa actggcgtaa ttcagcgggc agcggatcgc
    26281 ttgccgccat cgccagtggt ccgaaaagtc cggtttcctc cgcgcgttgc atccatgcag
    26341 ctgcaagcgg ttgaggatgc gaaggcggcg taatacgcag caaacaataa cgctgtggct
    26401 gtttaaccgt gaaacgcccg aggcgaatcc cttgagccac aacctgtggt cgttcattgc
    26461 cgcataatcc accgcataac gcatcgaatt gcgcgcggcg aagcacccat cctccggggc
    26521 cgaaaccact accctgacga tgcagcgtta acagttcgcc gctttccgtc atcaggttaa
    26581 tcgcccgtcg ccacactccc gctaaccgcc aggactggcg ataattcggt gcactacggc
    26641 tggcaagcag aggatggatg atcgtcattg cgcgttcctc aaccaatccc catgctttca
    26701 gccagtgctt ccagcgcctg ttcaaaacac gccagcggtg cccgcacagt gcctgcgcca
    26761 atctgcccga tccccgcctc tttatgggcg ataccggtat tgatgagtgg cgtaatgccg
    26821 gtttctacca cgcgacgaat atccagcccc aggcacgcgc cctggaaatc ccagccgggg
    26881 atctgcaact gcatattgcg ctcaaggtaa atttccgcca tctcttcaga taccgctctg
    26941 gccgcttcca tgccacccgc accgacaaag cgcgttacgc caggcgctgc gatcattgcc
    27001 gcacctccga taccaaaggt ttcggtaatc gcgctatcgc ccatatccgg gttcgcctgc
    27061 tcctgcgaga agccggtgaa aaacagacct tgcggagtgt ttacaggcgc agtaaaccag
    27121 cgttcgccca gcccgcttac ccgaatcccg aacatattgc cgttgcgggt cattgccgtg
    27181 acgatgctgc ctgcgcggat catcgcgcca gcatccatcg ccgccttgca gtaagccatc
    27241 gcgaggttga ggaagaactg atcggtcacg ctgaggaaat ccatcacttc ggcgatgtgc
    27301 tgtttatcat gatcgaggcg agcaatttgt ggggccagcg cacgcattaa cagtgcagag
    27361 gaagcaatat tgcgttgatg gaactcatcg cccatcgtaa tgccctgcgc catcatcgcc
    27421 gtgagatcga taccgcgctc catgcgcccc agcgccgcgc ttaatactgg cattaacaca
    27481 tcgcgcatcc agcgatggcg agtcaggaca tcttcgccgt aagcgccaaa acgcatcact
    27541 ttgccgatac cttcgttgag gttgcagtac gcccggttgc cgtcggtcac gttctcgacc
    27601 accagcatcg gcatactggc agaagtaata ccgcccattg gaccgacggc attcacatgg
    27661 tgacaaggaa tgaagttcac ttccccctgc tccagtattg ccagcgcctg cgcttcatct
    27721 ttcgcccagc cttcgaacag acatgcgccc acgcacgccc ctttcatggg tccggtcatc
    27781 tcctgccagc gcattggcgg cccggcgtga agcagtgttt tgccctcgtt tagttcgctg
    27841 ataagtgaag aagccggttg cacatccagc cagtgtggac gagcacgacg aatttgttcg
    27901 ataaccgcag cattggcttg cgccactgat gtaaacatgg gaacccctta ttgcaaacgt
    27961 tctaataaac gagccagttt tttattgccg ccggcgactg gcgaccattg gtaatgcaca
    28021 accggtttgc tggcgctttg tagctccagc gcaaagctgc gtaatccgat gttaatcacg
    28081 gcgacgtttt ccagtaatga cggtgtgtgt tgctgtgtgg cagacgagag cggatgaatt
    28141 aacgctgccg ccagcaaggt ggcttccggt agcgaactca ctaccgcgat ccccgcatct
    28201 tccagcgtgg cgatttgctg cgagcggcat tgcgggtcac gttcggtacc tgtcaccgtg
    28261 gcaatggcgt acagcggttg gctgtctgaa cgcgcggcac aggctttttg ccaggcgctc
    28321 accagcgagg cagcaggatc ggcggtcgca ccgaagccaa tcacgacatc aagcagcaac
    28381 acgcgcactt gcggtttagc gccgagatcg gcaattaact ggttgcgtaa ggctgggtcg
    28441 atcatcggat ggggacgccc gacggtgtag aaatcatcgc ccagatcaat gatctggtga
    28501 ccatcggcgt ccagcatcat gccgtgatga tgggtatcgt cggcttccac gccaaggtgc
    28561 ccggcaagta atcccgccgc ttcggcagca agcgtaccgc cggtatacaa accgcaaata
    28621 aatccgctgc tgacaggcgc tattgcgtta cgtcgcgccg tgacgcgtga aagcaggcaa
    28681 gccaggcgtg cggcctcatc cagcgaggag gcaaaccaga cattctcgtc gcgggccacc
    28741 gccggggtat aacctaaaaa cagcgccacc gtcggtttgc cggttgcttt catggcatta
    28801 acaattttca gacgcacagc ttcggcaggt ggttttgaaa caaatgccag cacttcgctt
    28861 ttctcgtctg cactgagcat ttccagcgct gttagcgcac tgatgccgcc cacttcacgg
    28921 ctgaggtcgc gcccgccaag gccaatcgcg tgagtaattc cctcccctgc cagcgcaatc
    28981 tgcgaacaca gctcctgaat cccggtaccg gaagcgccaa tgacgccaat attgccttct
    29041 ggcatcacgt tagcaaaagc cagcggtgtg ccggcaatca tcgacgtacc gcagtccggc
    29101 cccatcacca gcaagccttt ttcccgcgcg cgggttttaa gttggatttc atcttccagc
    29161 gtgacgttat cggagaacat catcacgttg aggttgcgat ccagcgcctg gtttgtcagc
    29221 tccgccgcat actcgccagc cactgaaatc agcgccagat tggcatcggg taatttctga
    29281 caggcgctgt cccagcgacg cacctgcgtc aacgcctggc tgctgcctga cccctgcgcc
    29341 agttgtttta gcgcctcttc aagctgctgc ataatcgcct gcgcgatccc cgcatccgcc
    29401 gcttcgctac gaattgccac gcaaatatcg ttcggcgtgg cgttattaaa atcgtcatgc
    29461 cagaaacctg tggtatctaa taacgcttta ttggcgggcg tacccatcat tacggaaaca
    29521 tcatcaacat tttctgattc gctgagtttt cgtgaaataa tcattaaact gaccgaatcc
    29581 tgaaaacacc cttttttaat aaaggcgtgg atcataacaa ctccttaaaa attgcaccca
    29641 tggcaattgc acgagcagtg ttaatgtggc gattacggta tcgatgacaa cgagtaaata
    29701 aggtgatgaa tatcacacag ggattattaa caaaagaatt caaataaccg ccaggctatt
    29761 acgcattatt ttttaataag cgtggttgtt ttaaaaatca ggttaaaagc gcgaagatta
    29821 tcaaataaat tctaactata ttttttgcct gtctggatca cataatccag atattttccc
    29881 tgtatgttaa ttgcagtcat gcttcacacc gtcgttaaaa aggaagacag atgaaaatca
    29941 gtcgggaaac actccaccag ctaattgaga ataaactctg ccaggctggg ttaaaacgtg
    30001 agcacgctgc aaccgtggct gaagtattgg tttacgccga tgccagaggg atccactctc
    30061 atggcgcggt gcgcgtggaa tactacgcgg aacgcatttc aaaaggcggc accaaccgcg
    30121 aaccggagtt tcgtcttgag gaaaccgggc cgtgctcggc aattttacat gccgacaatg
    30181 ccgccggaca ggtcgcggcg aaaatgggta tggaacatgc catcaaaacc gcccagcaaa
    30241 atggcgttgc ggtggtcggt atcagccgga tgggtcacag cggcgcaatc tcttattttg
    30301 tgcagcaggc agcccgcgcc ggattaattg gtatttcgat gtgccagtcc gatccaatgg
    30361 tggtgccgtt tggcggcgcg gaaatttact acggtactaa cccactggcc tttgccgcgc
    30421 cgggagaagg cgacgagatc cttacctttg atatggcgac taccgtacag gcatggggaa
    30481 aagtgctcga cgcccgctcg cgtaatatgt ctatcccgga tacctgggcg gtcgataaaa
    30541 acggtgcacc aacaaccgat ccgttcgcgg tacatgctct gctccccgcc gccgggccaa
    30601 aagggtatgg cctgatgatg atgattgacg tcctctcagg cgtcttactc ggcttaccgt
    30661 tcgggcgaca ggttagttcg atgtatgacg atttacacgc cgggcgtaat ttggggcaat
    30721 tacatatcgt tattaacccg aactttttct tctccagcaa attatttcgt caacatctta
    30781 gccagaccat gcgcgaatta aatgccatta cccccgcgcc cggttttaat caggtttatt
    30841 atcccggaca ggatcaggat attaaacaac gcaaagccgc cgtcgaaggc atcgaaattg
    30901 ttgatgatat ttaccagtat ttaatttccg acgcgcttta taacacgtca tacgaaacga
    30961 aaaatccctt tgcgcaataa ttattgagac aggactttct tatgattaca cattttcgtc
    31021 aagccataga agaaacgctg ccctggcttt cctcttttgg cgctgaccca acgggtggta
    31081 tgacccgttt actttattcg ccggaatggc tggaaactca gcagcaattt aaaaaaagaa
    31141 tggcagcaag cgggctggaa acacgtttcg atgaagtggg gaatttatat ggtcgcctga
    31201 gtggcaccga atatccacaa gaagtggttc tgagcggttc gcatatcgat accgtggtta
    31261 acggcggtaa cctcgacggg caattcggcg cgctggcggc gtggctggca attgactggc
    31321 tgaaaacgca atacggcgcg ccgctacgta cggtcgaagt ggtggcgatg gcagaagaag
    31381 aaggcagccg cttcccgtat gtcttctggg gcagtaaaaa tatctttggg ctggcgaatc
    31441 ctgacgacgt gcggaatatc tgtgatgcca aaggaaatag ttttgtcgat gcgatgaagg
    31501 cttgcggatt tactcttccg aacgccccac taactccgcg tcaggatatt aaagcctttg
    31561 ttgaactgca tattgaacag ggctgtgtgc tggaaagtaa tgggcaatca attggcgtgg
    31621 tgaatgcaat tgtcgggcag cgtcgttata cggtaacgct gaacggcgaa tcaaaccatg
    31681 caggcaccac gccgatgggt tatcgtcgtg atacagttta cgctttcagt cgcatttgcc
    31741 atcagtcggt cgaaaaagcg aaaaggatgg gcgatccgct ggttctgacc tttggcaaag
    31801 tagagccgcg cccgaatacg gtaaatgtgg tgccgggtaa aaccacgttc accattgatt
    31861 gtcgtcatac cgacgctgcc gtgctgcgcg atttcaccca acagttagaa aacgacatgc
    31921 gggcgatttg cgatgaaatg gacattggta ttgatatcga tttatggatg gacgaagaac
    31981 ccgtgccgat gaataaggag ctggtcgcca ccctgacaga attgtgtgaa agagaaaaac
    32041 tgaattaccg ggtgatgcac agtggtgccg ggcacgacgc gcaaattttc gcgcctcgcg
    32101 taccaacctg catgattttc attcccagca tcaacgggat cagccataac ccggcggaac
    32161 gcaccaatat taccgacctt gccgaagggg tcaaaacgtt ggcactcatg ctttatcaac
    32221 ttgcctggca gaaataagga gtcataaatg ggatatttaa ataacgtcac cggttaccgc
    32281 gatgatttac tggctaaccg tgcgattgtt aaacacggta atttcgcact gttaaccccg
    32341 gatggtctgg taaaaaatat tattccgggc tttgaaaatt gtgacgcgac aatcctctcc
    32401 acgccaaagc tgggtgcctc ttttgttgat tatctggtca cactgcatca aaacggtggc
    32461 aaccaacagg gcttcggtgg cgaaggcatt gaaacgttcc tgtatgtgat ctctggaaat
    32521 atcactgcca aagccgaagg caaaacattt gccttaagcg aaggtggcta tctttattgc
    32581 ccaccaggct ccttaatgac gtttgttaac gcccaggccg aagacagcca aatcttttta
    32641 tataagcgcc gctatgttcc ggtagaaggc catgcaccgt ggctggtttc tggtaatgcc
    32701 agcgaactgg aacgcattca ctatgaaggt atggatgatg tgatcctgct ggatttcctg
    32761 cccaaagagt taggttttga tatgaacatg catatcctct cttttgcgcc aggtgccagc
    32821 cacggttata tcgaaacaca cgttcaggaa catggtgcct atattctttc cggtcagggg
    32881 gtttataacc tcgacaataa ctggatcccg gtgaaaaaag gcgattacat ctttatgggc
    32941 gcttattctt tacaggctgg ttatggcgta gggcgtggtg aagcgttcag ctatatttac
    33001 tcgaaagatt gcaaccgcga cgtagagatt taagtcatac cactcgcctg attattttta
    33061 gctatgttgg attttgccac ggtttatacc gtggcttttt ttgtagtaaa ttcaggtaaa
    33121 aaaagatgcg aaataaatcg catccgcttt aaaggtaagg gtcagttttt aattccctga
    33181 cctattttaa tggcgcaggc aatatttcgc gcgctgttga agagattggt ttcaccgctg
    33241 gcgagcactt cggctaaagg tgccagacga ggcaaaatgc tgaataccgc gtcaatgccg
    33301 tactggtgca ccacttccac gccatcaccc aatacgccag caatcccaat caccgggaca
    33361 ttaaactgct tcgccaccga cgcaacgccc agcggcgctt taccgcctgc cgtttgcgag
    33421 tcaatgcgcc cttccccggt gatcaccagt gctgcgccct gcaccgcctg ctccaggttg
    33481 accgcccgca gtacaatctc aatacccggt ttaatatccg cattgaggaa taccgccgcc
    33541 gcaataccca taccgcccgc agcgccgccg cccgccatct ggcagacatt aatttcagtt
    33601 tgctgttgta aaacacgggc gtaattttgc agcccctgtt caagttcttc gaccatctcc
    33661 ggcgttgccc ctttttgcgg gccaaacacc gccgccgcgc cgcgtgcccc taccagcggg
    33721 ttatcaacgt cacatgctac ttcaatatgg caattcgcca gacgcggatc gcattcatcc
    33781 atctcaatgc ttgccacgcg cgctaaatta ccgccgttag ccgccagcac ctggccgtcg
    33841 gcatcaagga aacgcacacc aagcgcctga gccatgccca taccgccgtc gaccgtcgca
    33901 ctgccgccaa tgccgagaat aatatggcga atgccgttat ccagcgcatg acgaattaac
    33961 tcccccgtac caaaactgga ggccagcaac ggattacgct tttcaggcgc gaccagcatc
    34021 aggccacttg ctgccgccat ctcaatcacc gccgttttcc cgtcgccggt aaggccataa
    34081 aaagcattca ctgtttcgcc catcggcccg cagacttcaa gcgtcacgat gttgccgccc
    34141 gtcgcggcga ccatcgcatc caccgtccct tcgccgccat ccgctatcgg caaacagata
    34201 tagttcgcat cgggaaagag ggtcgaaaac ccggctttaa ttgcctgaca acatttttct
    34261 gcacttaagc tctctttaaa agagtctggc gcaatgacaa tcttcattat caatcctttt
    34321 tcaacaaatt attcgatgcg ttcccacgga ataagattat ccataagaac agccagtaaa
    34381 attccgacca gtaagccgtt actgagcaga ggacgcagcg ttaatggaag gtcttgcaga
    34441 tacacaggcg gtaatgccat taaaaatatg ccgacaaata acggcaatgc gagtcgataa
    34501 atattgcgag cggtaaaggt tatttgctgg ctaaacacca gcgcggaaaa gagtaaaggc
    34561 agataagaaa ccagcatgac cgcgctactc acgggtaaag ggatactgca aaacagtcgt
    34621 gtgagtgccg gaaccagcgc caccagcagg caaataacgc tgccataaat aaacgaacgc
    34681 cgcgtgtaat cgcctgtctg ggttaataaa ccaatggatg aaacaaacgg tgaaaatgga
    34741 attaccgcca gcggtacggt tatcagcgtc ataaatccgg tcgccacaaa gctgcgacga
    34801 taacgcgtat tcccgcgccc tgctgcggat aaaaaacatc cgtgccccga atcgcaccgt
    34861 aggtattgct gatattcacc agacctgtaa tcaccgccgt cagaataatt cccggagaca
    34921 aagcacctcc actgccgagc gggaaccact gccagtgcaa ctcaccggag agcgagtgcg
    34981 aagaaggaaa gcaaaagtac cacaacaacc agccggttat ggtgccgacc agcaggccat
    35041 aacgggcaaa acgttgcggc aggaagataa tcatccccag taccaggcac atcaccgcca
    35101 ccgagagcgc gaacggcggt aactgaattt taaaatttgg gtcggctatg ccaaacggca
    35161 gcccgagcat acctttgaaa aagatagtgg tcagctgcgc gcccagcatc aacataaaca
    35221 agaccatcac cgacggcgta aataaccgtg ccaggcgatg gcctaatccg ctaaaaccaa
    35281 tcaacatcgt cagcacgccg gagagcgcaa tccccaccgc caggctggta gcgatatcgt
    35341 tgatcggtgt cccgcgcgat gcttcgccaa gggtgatagt aaggatggtt ccccaccaca
    35401 ggccacccgg cccttccata atagcgcgac gatgaccgca gaacgcctga gcgaagcagg
    35461 ccagtgcggt agcaagaaaa gcatattgcg tgagcgtaag caggctactt tgcggcaact
    35521 gaaaagcaga aagtagcgta ggaggaacca caaccgtgtt gcaaaaaata aagaaaaacc
    35581 actgaaatcc tgataacagg ctttcgcggc tcactgcaaa attgaacata aacacacctt
    35641 aaccggagaa tgcccgccgc aaggacgggc attgcagggg ccagattact gctgatgttt
    35701 aaggataaat tgacctttcg gcgcaacagg gaagccctgt tcaatgtcgt aaatcacatc
    35761 accacgtaag atggttttcg tgatacgcgc gccaatggta cggccaacat acgggctgac
    35821 tttgtggcga tattccagat cgtcattggt aagaacatag ctgctattcg gctgaatgaa
    35881 gacgaagtcg gcatcttttc ctggggcgat acggcctttt tgctgcagac cgaaaatatc
    35941 tgctgcgtta gtcgccatta atttgccgaa cattggcaga gacattccgc gtttctgtac
    36001 cgcttcatcg aacatcacgt ccatacagtt ttgcagaccg gcaataccgc cccatgcttc
    36061 catgatgttg ccggctttca tttccggagg gcatggtgag tggtcggaaa ccaggcagtc
    36121 gatttcaccg ttaaacagtt tttcccacat gcctttctgg ttttccagat cgcggatcgg
    36181 cggtgaacac ttcgccagag tgccgatttc ttcgaactga tcggtatcca gtacaaagta
    36241 atgcgggcag gattcacaag taacgtcctg accttcctga cgtgcacgag tcacttcttc
    36301 aacgccttcc gggctgctga tatggcaaac gtgcagacgg caaccggcaa ctttcgccag
    36361 atacagcacg cggcgaatcg cttccacttc ggtaaatacc ggacgcgaag ccacatagtc
    36421 atgggcagtt acgcgacctt cacgtttcgc ttcttcgccc agtgcgtcac agatcagcgc
    36481 gttttcgcag tgcaccagca ccggctgccc cagttcgccc agcttctgcg cacctttgaa
    36541 gaactgccag tcgtttacat cacggaagtc gttgtcgata ccgcgatcgc cacaggtcgc
    36601 aacgaagcat ttgaagccga caacgcccac ttcatccagc tcatgcagac ggtcgatgtt
    36661 gtaagacacc aggccaccga gttgtgccgc atcgatagtc agcttgcctt tagcggcatc
    36721 gaacttcagt tcaattgaag cgcggtcaac cgttgcaggc agctggttga gcggcatttc
    36781 gatcatggtg gtgataccac cttttgccgc tgcgcgagta ccggtttcat aaccttccca
    36841 gtggctacga cccggttcag aaatatgggt gtgcgcatca accatgcccg gcgaaaccac
    36901 cagaccagac gcatccataa cttcttttgc atcgcccaga tcctgaccga tagcagcaat
    36961 tttcccgcct ttaacggcga tatctacaac gcgagcttcg ttttctaaaa taacggtgcc
    37021 gtttttaatg attaaatcaa aagacataac aaactccttg taaaatacag attggttttt
    37081 aactcaatgt tcaatatcgg gattaattaa cctatggttt tttgctctcc tgttttttct
    37141 gctgttgtac gtttctttaa taaggcgtag gccgcaaagg cgacgatgac gccgacaaac
    37201 catgaaacac gcgataacgg ttccataaag tgaataaact taccgccaag agataaaata
    37261 acggccacca gagttactga aaacgcagtg aggttaaaac cgttatcgta atatttataa
    37321 tcgccaggtg cggtatacag ttcatcaaga ttaatttgtc cgcgcatcac cacaaaataa
    37381 tgcgccatca tgacaccaat taccggacca agcattccgc cgataatatc gaggaaaaga
    37441 taaatgctgt cctgattttc cattaatttc cacgggcaga tcagcaagct gataatactg
    37501 gcaatcagta cgccgttttt ataggtcagt tttgtcggtg caatggcggc aatctgataa
    37561 ccggctggaa taatattacc ggtcgcgtta gtggagatag ttgtcatcag aatgaccagt
    37621 accgcaaaga acgaggcgaa caggctgtcc caacgctgaa caatatccag cacgttccag
    37681 gtatcagcgc cgtagtgaat actggctccg gcaataatac ataccccggc gaccgcaaac
    37741 agaatatagg ccacaactaa acccagcgtt tgccccagcg cctgctcacg aaacgagtgg
    37801 gcgttttgcg taaagtcgga tgcgctcacc gccggtgccg cccagaccgc aactaccgcg
    37861 ttaatcacca ccaggaacag gaagccaccg ttttctgctt tctgaatacc gctcggaatg
    37921 tagtcaaaga ttggaccgat cccgaccagt gaaatcgccc aaatcgccat accgccgaaa
    37981 acgatataga tgcacgggtt aagaatggca gtgaatttat ttaaaacttt gccaccgcca
    38041 aaacctatac caacgttgac cagccagaag attaagaaag taattaagcc cggtagagaa
    38101 aggcctaaca gagtgaaatc accaccgaga gttaaaaatc ccggccagat tttgccaatc
    38161 agaatcaagc aggccagtga ccccgcgtaa cattgcaaac caaaccacat gatggcggca
    38221 attccgcccc ttaataatcc gggaaacagt gcaccacgta caccgtaaga agcacgcagg
    38281 atcatggcaa aaggcacacc gtatttactg cccgcagcac cgtttaatac cattaccgcg
    38341 gcaatgaaaa aggcgctgag gataattgcc agcataatac taaaggtaga caagccgaga
    38401 ataaaaaagc cgccgaccat cacataattg ggaacgttat gaaccgaacc catccataag
    38461 gtaaaatagt taaatgtttt ccaggtccgc tggctttgcg ttttcggcaa tagatcttcg
    38521 ctatagccgc gttgctggaa tagttttctc tgatgttcca taaatccctc ttaatttcac
    38581 gt
//
LOCUS       DAZGOB010000038        37129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGOB010000038 DAZGOB010000000
VERSION     DAZGOB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.038"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            547..2115
                     /gene="cydA"
                     /locus_tag="ACHLIF_001999"
     CDS             547..2115
                     /gene="cydA"
                     /locus_tag="ACHLIF_001999"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO9606023.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            2131..3270
                     /gene="cydB"
                     /locus_tag="ACHLIF_002000"
     CDS             2131..3270
                     /gene="cydB"
                     /locus_tag="ACHLIF_002000"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO9606024.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3285..3398
                     /gene="cydX"
                     /locus_tag="ACHLIF_002001"
     CDS             3285..3398
                     /gene="cydX"
                     /locus_tag="ACHLIF_002001"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO9606025.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3398..3691
                     /gene="ybgE"
                     /locus_tag="ACHLIF_002002"
     CDS             3398..3691
                     /gene="ybgE"
                     /locus_tag="ACHLIF_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO9606026.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3841..4245
                     /gene="ybgC"
                     /locus_tag="ACHLIF_002003"
     CDS             3841..4245
                     /gene="ybgC"
                     /locus_tag="ACHLIF_002003"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO9606027.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4242..4934
                     /gene="tolQ"
                     /locus_tag="ACHLIF_002004"
     CDS             4242..4934
                     /gene="tolQ"
                     /locus_tag="ACHLIF_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO9606028.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4938..5366
                     /gene="tolR"
                     /locus_tag="ACHLIF_002005"
     CDS             4938..5366
                     /gene="tolR"
                     /locus_tag="ACHLIF_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO9606029.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5431..6696
                     /gene="tolA"
                     /locus_tag="ACHLIF_002006"
     CDS             5431..6696
                     /gene="tolA"
                     /locus_tag="ACHLIF_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO9606030.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6829..8121
                     /gene="tolB"
                     /locus_tag="ACHLIF_002007"
     CDS             6829..8121
                     /gene="tolB"
                     /locus_tag="ACHLIF_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO9606031.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            8156..8677
                     /gene="pal"
                     /locus_tag="ACHLIF_002008"
     CDS             8156..8677
                     /gene="pal"
                     /locus_tag="ACHLIF_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO9606032.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8687..9478
                     /gene="cpoB"
                     /locus_tag="ACHLIF_002009"
     CDS             8687..9478
                     /gene="cpoB"
                     /locus_tag="ACHLIF_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO9606033.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9643..9718
                     /locus_tag="ACHLIF_002010"
     tRNA            9643..9718
                     /locus_tag="ACHLIF_002010"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9676..9678,aa:Lys,seq:ttt)
     gene            9854..9929
                     /locus_tag="ACHLIF_002011"
     tRNA            9854..9929
                     /locus_tag="ACHLIF_002011"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9887..9889,aa:Val,seq:tac)
     gene            9932..10007
                     /locus_tag="ACHLIF_002012"
     tRNA            9932..10007
                     /locus_tag="ACHLIF_002012"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9965..9967,aa:Lys,seq:ttt)
     gene            10157..10232
                     /locus_tag="ACHLIF_002013"
     tRNA            10157..10232
                     /locus_tag="ACHLIF_002013"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10190..10192,aa:Val,seq:tac)
     gene            10236..10311
                     /locus_tag="ACHLIF_002014"
     tRNA            10236..10311
                     /locus_tag="ACHLIF_002014"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10269..10271,aa:Lys,seq:ttt)
     gene            10458..10533
                     /locus_tag="ACHLIF_002015"
     tRNA            10458..10533
                     /locus_tag="ACHLIF_002015"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10491..10493,aa:Lys,seq:ttt)
     gene            10567..10642
                     /locus_tag="ACHLIF_002016"
     tRNA            10567..10642
                     /locus_tag="ACHLIF_002016"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10600..10602,aa:Lys,seq:ttt)
     gene            10674..10798
                     /locus_tag="ACHLIF_002017"
     ncRNA           10674..10798
                     /ncRNA_class="other"
                     /locus_tag="ACHLIF_002017"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11047..12090
                     /gene="nadA"
                     /locus_tag="ACHLIF_002018"
     CDS             11047..12090
                     /gene="nadA"
                     /locus_tag="ACHLIF_002018"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO9606034.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            12128..12847
                     /gene="pnuC"
                     /locus_tag="ACHLIF_002019"
     CDS             12128..12847
                     /gene="pnuC"
                     /locus_tag="ACHLIF_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO9606035.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRLAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12844..13785)
                     /gene="zitB"
                     /locus_tag="ACHLIF_002020"
     CDS             complement(12844..13785)
                     /gene="zitB"
                     /locus_tag="ACHLIF_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO9606036.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13899..14279)
                     /gene="ybgS"
                     /locus_tag="ACHLIF_002021"
     CDS             complement(13899..14279)
                     /gene="ybgS"
                     /locus_tag="ACHLIF_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO9606037.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14595..15647
                     /gene="aroG"
                     /locus_tag="ACHLIF_002022"
     CDS             14595..15647
                     /gene="aroG"
                     /locus_tag="ACHLIF_002022"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO9606038.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15806..16558)
                     /gene="gpmA"
                     /locus_tag="ACHLIF_002023"
     CDS             complement(15806..16558)
                     /gene="gpmA"
                     /locus_tag="ACHLIF_002023"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO9606039.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16761..17801)
                     /gene="galM"
                     /locus_tag="ACHLIF_002024"
     CDS             complement(16761..17801)
                     /gene="galM"
                     /locus_tag="ACHLIF_002024"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO9606040.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17795..18943)
                     /gene="galK"
                     /locus_tag="ACHLIF_002025"
     CDS             complement(17795..18943)
                     /gene="galK"
                     /locus_tag="ACHLIF_002025"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO9606041.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYYLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18947..19993)
                     /gene="galT"
                     /locus_tag="ACHLIF_002026"
     CDS             complement(18947..19993)
                     /gene="galT"
                     /locus_tag="ACHLIF_002026"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO9606042.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(20003..21019)
                     /gene="galE"
                     /locus_tag="ACHLIF_002027"
     CDS             complement(20003..21019)
                     /gene="galE"
                     /locus_tag="ACHLIF_002027"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO9606043.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(21280..22752)
                     /gene="modF"
                     /locus_tag="ACHLIF_002028"
     CDS             complement(21280..22752)
                     /gene="modF"
                     /locus_tag="ACHLIF_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO9606044.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(22820..23608)
                     /gene="modE"
                     /locus_tag="ACHLIF_002029"
     CDS             complement(22820..23608)
                     /gene="modE"
                     /locus_tag="ACHLIF_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO9606045.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23737..23886
                     /gene="acrZ"
                     /locus_tag="ACHLIF_002030"
     CDS             23737..23886
                     /gene="acrZ"
                     /locus_tag="ACHLIF_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO9606046.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            24052..24825
                     /gene="modA"
                     /locus_tag="ACHLIF_002031"
     CDS             24052..24825
                     /gene="modA"
                     /locus_tag="ACHLIF_002031"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9606047.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEEHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24825..25514
                     /gene="modB"
                     /locus_tag="ACHLIF_002032"
     CDS             24825..25514
                     /gene="modB"
                     /locus_tag="ACHLIF_002032"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO9606048.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25517..26575
                     /gene="modC"
                     /locus_tag="ACHLIF_002033"
     CDS             25517..26575
                     /gene="modC"
                     /locus_tag="ACHLIF_002033"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO9606049.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26576..27394)
                     /gene="ybhA"
                     /locus_tag="ACHLIF_002034"
     CDS             complement(26576..27394)
                     /gene="ybhA"
                     /locus_tag="ACHLIF_002034"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO9606050.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27549..28544
                     /gene="pgl"
                     /locus_tag="ACHLIF_002035"
     CDS             27549..28544
                     /gene="pgl"
                     /locus_tag="ACHLIF_002035"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO9606051.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28585..29538)
                     /gene="ybhD"
                     /locus_tag="ACHLIF_002036"
     CDS             complement(28585..29538)
                     /gene="ybhD"
                     /locus_tag="ACHLIF_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9606052.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29722..30774
                     /gene="ybhH"
                     /locus_tag="ACHLIF_002037"
     CDS             29722..30774
                     /gene="ybhH"
                     /locus_tag="ACHLIF_002037"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO9606053.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30850..32283
                     /gene="ybhI"
                     /locus_tag="ACHLIF_002038"
     CDS             30850..32283
                     /gene="ybhI"
                     /locus_tag="ACHLIF_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO9606054.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            32466..34727
                     /gene="ybhJ"
                     /locus_tag="ACHLIF_002039"
     CDS             32466..34727
                     /gene="ybhJ"
                     /locus_tag="ACHLIF_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO9606055.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34961..36244)
                     /gene="ybhC"
                     /locus_tag="ACHLIF_002040"
     CDS             complement(34961..36244)
                     /gene="ybhC"
                     /locus_tag="ACHLIF_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO9606056.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(36379..>37129)
                     /locus_tag="ACHLIF_002041"
     CDS             complement(36379..>37129)
                     /locus_tag="ACHLIF_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9606057.1"
                     /translation="RRGLPDAPLEDITTKEIAAMLSGYIDEGKAASAKLIRSTLSDAF
                     REAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVT
                     GQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILG
                     GETIIASTHREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDK
                     FAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         8945 a         9075 c         9738 g         9371 t
ORIGIN      
        1 attagtaatc ctgaaactct gcgtcgtatt agccagtgac caaaaaaaga attaaggtca
       61 accgtgctgt ttttgcttcg tctcttttta tctttaattg ccaaccgaaa ctaatttcag
      121 ccttataact cacacatttt aaacataaat gtcactaaag ttaccttatt gaaacatgat
      181 taacataatt tgtaggaatt gatatttatc aatgtataag tcttggaaat gggcatcaaa
      241 aagagataaa ttgttctcga tcaaattggc tgaaaggcgg taatttagct ataaattgat
      301 caccgtcgaa aaatgcaaat ttgcttcaac aaaaacctgt ttattgtaag gattttgcgg
      361 cgtaatatat acgtgggatc aatttgagtt tttattaaca tgtttgcaac ctttctttac
      421 gccgtttttg tgtgcattca catggtatga tgaaagtgtt caaacaaatt tctattgggg
      481 catgcgtgtg accctttcta acggggttca ctctcggagt cttcatgcga tgagcaagga
      541 gtcatgatgt tagatatagt cgaactgtcg cgcttacagt ttgccttgac cgcgatgtac
      601 cacttccttt ttgtgccact gacgctcggt atggcgttcc tgctggccat tatggaaacg
      661 gtctacgtcc tctccggcaa acagatttat aaagatatga ccaagttctg gggcaagttg
      721 tttggtatca acttcgctct gggtgtggct accggtctga ccatggagtt ccagttcggg
      781 actaactggt cttactattc ccactatgta ggggatatct tcggtgcgcc gctggcaatc
      841 gaaggtctga tggccttctt cctcgaatcc acctttgtag gtctgttctt cttcggttgg
      901 gatcgtctgg gtaaagttca gcatatgtgt gtcacctggc tggtggcgct cggttcaaac
      961 ctgtccgcac tgtggattct ggttgcgaac ggctggatgc aaaacccaat cgcgtccgat
     1021 ttcaactttg aaactatgcg tatggagatg gtgagcttct ccgagctggt gcttaacccg
     1081 gttgctcagg tgaaattcgt tcacactgta gcgtctggtt atgtgactgg cgcgatgttc
     1141 atcctcggta tcagcgcatg gtatatgctg aaaggtcgtg acttcgcctt cgctaaacgc
     1201 tcctttgcta tcgctgccag cttcggtatg gctgctgttc tgtctgttat tgttctgggt
     1261 gatgaatccg gctacgaaat gggcgacgtg cagaaaacca aactggctgc tattgaagcc
     1321 gagtgggaaa cgcaacctgc gcctgctgcc tttactctgt tcggcattcc tgatcaggaa
     1381 gaggagacga acaaatttgc gattcagatc ccttacgcac tgggcatcat tgcaacgcgt
     1441 tccgtggata ccccggttat cggcctgaaa gagctgatgg tgcagcatga agaacgcatt
     1501 cgtaacggga tgaaggcgta ctctctgctc gaacaactgc gttctggttc taccgaccag
     1561 gcggttcgtg accagttcaa tagcatgaag aaagacctcg gttacggtct gctgctgaaa
     1621 cgctatacgc caaacgtggc tgatgcgact gaagcgcaga ttcaacaggc aaccaaagac
     1681 tccatcccgc gtgtagcgcc gctgtacttt gcgttccgta tcatggtggc gtgtggcttc
     1741 ctgcttctgg caatcatcgc gctctctttc tggagtgtca tccgcaaccg cattggcgag
     1801 aaaaaatggc ttctgcgcgc cgcgctgtac ggtattccgc tgccgtggat tgctgtagaa
     1861 gcgggctggt tcgtggctga atatggccgc caaccgtggg ctatcggtga agtgctgccg
     1921 acagctgtgg cgaactcgtc actgaccgca ggcgatctca tcttctcaat ggtgctgatt
     1981 tgcggcctgt ataccctgtt cctggtggca gaattgttct taatgttcaa atttgcacgc
     2041 ctcggcccaa gcagcctgaa aaccggtcgc tatcactttg agcagtcttc cacgactact
     2101 cagccggcac gctaagacag gagtcgtcaa atgatcgatt atgaagtatt gcgttttatc
     2161 tggtggctgc tggttggcgt tctgctgatt ggttttgcag tcactgacgg tttcgacatg
     2221 ggggtgggca tgctcacccg tttcctcggt cgtaacgaca ccgagcgtcg aattatgatt
     2281 aactccattg caccacactg ggacggtaac caggtttggc tgatcaccgc gggcggcgca
     2341 ctctttgctg cctggccgat ggtctatgcc gctgcgttct ccggcttcta tgtggcgatg
     2401 atcctcgtgc tggcgtcttt gttcttccgt ccggtcggtt ttgactaccg ctccaagatt
     2461 gaagaaaccc gctggcgtaa catgtgggac tggggcatct tcattggtag cttcgttccg
     2521 ccgctggtaa ttggtgtagc gttcggtaac ctgttgcagg gcgtaccgtt caacgttgat
     2581 gaatatctgc gtctgtacta caccggtaac ttcttccagt tgcttaaccc gttcggcctg
     2641 ctggcaggcg tggtgagcgt agggatgatc attactcagg gcgcaaccta tctgcaaatg
     2701 cgtaccgtgg gcgaactgca cctgcgtacc cgtgcaacgg ctcaggtggc tgcgctggtg
     2761 acactggtct gtttcgcact ggctggcgta tgggtgatgt acggtatcga tggttatgtc
     2821 gtgaaatcga caatggacca ttacgcagcc tctaacccac tgaataaaga agtggttcgt
     2881 gaagctggcg catggctggt taacttcaac aacacgccaa ttctgtgggc tattccggca
     2941 ctgggtgtgg ttctgccgct gctgaccatc ctgactgcac gtatggataa agccgcgtgg
     3001 gcgtttgtgt tctcctccct gacgctggcc tgcatcatcc tgacagccgg tatcgcaatg
     3061 ttcccgtttg tgatgccgtc cagcaccatg atgaacgcaa gtctgacaat gtgggatgca
     3121 acttccagcc agctgacgct taacgtcatg acctgggttg cggtggttct ggtaccgatc
     3181 attctgctct acaccgcctg gtgttactgg aaaatgttcg gtcgtatcac caaagaagat
     3241 attgaacgta acacccactc tctgtactaa gtaaggagct aaaaatgtgg tatttcgcat
     3301 ggattctggg aacgcttctt gcctgttcgt ttggggtaat caccgcgctg gcgcttgaac
     3361 acgtcgaatc aggcaaagcc ggtcaagaag acatctgatg agtaagatta tcgcgacttt
     3421 gtatgcggta atggacaagc gccccctgcg ggcgctttcc ttcgtgatgg cgcttctgtt
     3481 agcaggatgt atgttttggg acccatctcg ttttgccgcg aagaccagtg aactggaaat
     3541 ctggcatggt ttattgctga tgtgggccgt ctgtgctggt gtgattcacg gcgtgggctt
     3601 tcgtccgcag aaggttcttt ggcaagggat tttttgccca ttgcttgccg atattgttct
     3661 cattgtcggg ctgattttct tcttctttta aatcagaatt ctctttaaaa aattatgggc
     3721 cgctccaggc ccataaattt ttacgctccc ttaacttgcc ctcattccca aacctcaatc
     3781 gcgcgcgtat agtagcagcg tttaaaagct ctaacttttg ttgcattacc gggatgtaaa
     3841 gtgaatacaa cgctgtttcg atggccggtt cgcgtctact atgaagatac cgatgccggt
     3901 ggtgtggtgt accacgccag ttacgtcgct ttttatgaaa gagcacgcac agagatgctg
     3961 cgtcatcatc acttcagtca gcaggcgctg atggctgaac gcgttgcctt tgtggtacgt
     4021 aaaatgacgg tggaatatta cgcacctgcg cggctcgacg atatgctcga aatacagact
     4081 gaaataacat caatgcgtgg cacctctttg gttttcacgc aacgtattgt caacgccgag
     4141 aatactttgc tgaatgaagc agaggttctg gttgtttgcg ttgacccact caaaatgaag
     4201 cctcgtgcgc ttcccaagtc tattgtcgcg gagtttaagc agtgactgac atgaatatcc
     4261 ttgatttgtt cctgaaggct agccttctgg ttaaacttat catgttgatt ttgattggtt
     4321 tttcaatcgc atcctgggcc attattatcc agcggacccg tattcttaac gcagcggcgc
     4381 gcgaagccga agcgtttgaa gataaattct ggtctggaat cgaactctct cgcctctatc
     4441 aagagagtca ggggaaacgg gataatctga ctggttcgga acaaatattt tacagcgggt
     4501 tcaaagagtt tgtgcgcctg catcgtgcca atagccatgc gccggaagcc gtagtggaag
     4561 gggcgtcgcg tgctatgcgt atctccatga accgtgaact tgaaaatctg gaaacgcaca
     4621 ttccgttcct cggtacggtt ggctccatca gcccgtatat tggtctgttt ggtacggtct
     4681 gggggatcat gcacgccttt atcgccctcg gggcggtaaa acaagcaaca ctgcaaatgg
     4741 ttgcgcccgg tatcgcagaa gcgttgattg cgactgcaat tggtctgttt gccgctatcc
     4801 cggcagttat ggcctacaac cgcctcaacc agcgcgtaaa caaactggaa ctgaattacg
     4861 acaactttat ggaagagttt accgcgattc tgcaccgcca ggcgtttacc gttagcgaga
     4921 gcaataaggg gtaagtcatg gccagagcgc gtggacgagg tcgtcgcgaa cttaagtccg
     4981 aaatcaacat tgtaccgttg ctggacgtac tgctggtgct gttgctgatc ttcatggcga
     5041 cagcgcccat catcacccag agcgtggagg tcgatctgcc agacgctact gaatcacagg
     5101 cggtgagcag taacgataat ccgccagtga ttgttgaagt gtctggtatt ggtcagtaca
     5161 ccgtggtggt tgagaaagat cgcctggagc gtttaccacc agagcaggtg gtggcggaag
     5221 tgtccagccg tttcaaggcc aacccgaaaa cggtctttct gatcggtggc gcaaaagatg
     5281 tgccttacga tgaaataatt aaagcactga acttgttaca tagtgcgggt gtgaaatcgg
     5341 ttggtttaat gacgcagcct atctaaacat ctgcgtttcc cttgcttgaa agagagcggg
     5401 taacaggcga acagtttttg gaaaccgaga gtgtcaaagg caaccgaaca aaacgacaag
     5461 ctcaagcggg cgataattat ttcagcagtg ctgcatgtca tcttatttgc ggcgctgatc
     5521 tggagttcgt tcgatgagaa tatagaagct tcagccggag gcggcggtgg ttcgtccatc
     5581 gacgctgtca tggttgattc aggtgcggta gttgagcagt acaaacgcat gcaaagccag
     5641 gaatcaagcg cgaagcgttc tgatgaacag cgcaagatga aggaacagca ggctgctgaa
     5701 gaactgcgtg agaaacaagc ggctgaacag gaacgcctga agcaacttga gaaagagcgg
     5761 ttagcggctc aggagcagaa aaagcaggct gaagaagccg caaaacaggc cgagttaaag
     5821 cagaagcaag ctgaagaggc ggcagcgaaa gcggcggcag atgctaaagc gaaggccgaa
     5881 gcagatgcta aagctgcgga agaagcagcg aagaaagcgg ctgcagacgc aaagaaaaaa
     5941 gcagaagcag aagccgccaa agccgcagcc gaagcgcaga aaaaagccga ggcagccgct
     6001 gcggcactga agaagaaagc ggaagcggca gaagcagctg cagctgaagc aagaaagaaa
     6061 gcggcaactg aagctgctga aaaagccaaa gcagaagctg agaagaaagc ggctgctgaa
     6121 aaggctgcag ctgataagaa agcggcagca gagaaagctg cagccgacaa aaaagcagca
     6181 gaaaaagcgg ctgctgaaaa ggcagcagct gataagaaag cagcggcaga aaaagccgcc
     6241 gcagacaaaa aagcggcagc ggcaaaagct gcagctgaaa aagccgctgc agcaaaagcg
     6301 gccgcagagg cagatgatat tttcggtgag ctaagctctg gtaagaatgc accgaaaacg
     6361 gggggagggg cgaaagggaa caatgcttcg cctgccggga gtggtaatac taaaaacaat
     6421 ggcgcatcag gggccgatat caataactat gccgggcaga ttaaatctgc tatcgaaagt
     6481 aagttctatg acgcatcgtc ctatgcaggc aaaacctgta cgctgcgcat aaaactggca
     6541 cccgatggta tgttactgga tatcaaacct gaaggtggcg atcccgcact ttgtcaggct
     6601 gcgttggcag cagctaaact tgcgaagatc ccgaaaccac caagccaggc agtatatgaa
     6661 gtgttcaaaa acgcgccatt ggacttcaaa ccgtaatcgc gatgttgact gttcggacgg
     6721 tcaacatcag gcaccggttg ccacggggtt ctggtagttt tgtgtatttt agtttgttaa
     6781 cattctgcta aattatcgtg ggccatcggt ccagataagg gagatatgat gaagcaggca
     6841 ttacgagtag catttggttt tctcatactg tgggcatcag ttctgcatgc tgaagtccgc
     6901 attgtgatcg acagcggtgt agattccggt cgtcctattg gtgttgttcc tttccagtgg
     6961 gcggggcctg gtgcggcacc tgaagatatt ggcggcatcg ttgctgctga cttgcgtaac
     7021 agcggtaaat ttaatccgtt agatcgcgct cgtctgccac agcagccggg tagtgcgcag
     7081 gaagtacaac cagctgcatg gtccgcactg ggcattgacg ctgtagttgt cggtcaggtc
     7141 actccgaatc cggatggttc ttacaatgtt gcttatcaac ttgttgacac tggcggcgca
     7201 ccgggtactg tacttgctca gaactcgtac aaagtgaaca agcagtggct gcgttatgct
     7261 ggtcataccg ccagtgatga agtgtttgaa aaactgaccg gcattaaagg tgcgttccgt
     7321 actcgtattg cctacgttgt tcagaccaac ggcggtcagt tcccgtatga actgcgcgta
     7381 tctgactatg acggttacaa ccagtttgtc gttcaccgtt caccgcagcc gctgatgtct
     7441 ccggcgtggt caccagacgg ttctaaactg gcttatgtga ccttcgaaag cggtcgttcc
     7501 gcgctggtta ttcagacgct ggcaaatggc gctgtacgtc aggtggcttc attcccgcgt
     7561 cacaacggtg cacctgcatt ctcgccagac ggcagcaaac tggcattcgc cttgtcgaaa
     7621 accggtagtc tgaacctgta cgtaatggat ttggcttctg gtcagatccg ccaggtgact
     7681 gatggtcgca gtaacaatac cgaaccgacc tggttcccgg atagccagaa cctggcattt
     7741 acttctgacc aggccggtcg tccgcaggtt tataaagtga atatcaacgg cggtgcgcca
     7801 caacgtatta cctgggaagg ttcgcagaac caggatgcgg atgtcagcag cgacggtaaa
     7861 tttatggtaa tggtcagctc caatggtggg cagcagcaca ttgccaaaca agatctggca
     7921 acgggaggcg tacaagttct gtcgtccacg ttcctggatg aaacgccaag tctggcacct
     7981 aacggcacta tggtaatcta cagctcttct caggggatgg gatccgtgct gaatttggtt
     8041 tctacagatg ggcgtttcaa agcgcgtctt ccggcaactg atggacaggt caaattccct
     8101 gcctggtcgc cgtatctgtg ataataatta attgaatagt aaaggaatca ttgaaatgca
     8161 actgaacaaa gtgctgaaag ggctgatgat tgctctgcct gttatggcaa ttgcggcatg
     8221 ttcttccaac aagaacgcca gcaatgacgg cagcgaaggc atgctgggtg ccggcactgg
     8281 tatggatgcg aacggcggca acggcaacat gtcttccgaa gagcaggctc gtctgcaaat
     8341 gcaacagctg cagcagaaca acatcgttta cttcgatctg gacaagtacg atatccgttc
     8401 tgacttcgct caaatgctgg atgcacatgc aaacttcctg cgtagcaacc cgtcttacaa
     8461 agtcaccgta gaaggtcacg cggacgaacg tggtactccg gaatacaaca tctccctggg
     8521 tgaacgtcgt gcgaacgccg ttaagatgta cctgcagggt aaaggcgttt ctgcagacca
     8581 gatctccatc gtttcttacg gtaaagaaaa acctgcagta ctgggtcatg acgaagcggc
     8641 atactccaaa aaccgtcgtg cggtactggt ttactaagag aattgcatga gcagtaactt
     8701 cagacatcaa ctattgagtc tgtcgttact ggttggtata gcggccccct gggccgcttt
     8761 tgctcaggca ccaatcagta gtgtcggctc aggctcggtc gaagaccgcg tcactcaact
     8821 tgagcgtatt tctaatgctc acagccagct tttaacccaa ctccagcaac aactttctga
     8881 taatcaatcc gatattgatt ccctgcgtgg tcagattcag gaaaatcagt atcaactgaa
     8941 tcaggtcgtg gagcggcaaa agcagatcct gttgcagatc gacagcctca gcagcggtgg
     9001 tgcagcggcg caatcaacca gcagcgatca aagcggtgcg gcggcatcaa cgacgccgac
     9061 agctgatgct ggtactgcga atgctggcgc gccggtgaaa agcggcgatg caaacacgga
     9121 ttacaatgca gctattgcgc tggtgcagga taaatcccgc caggatgacg caatggtggc
     9181 atttcagaat ttcatcaaaa attaccctga ttcaacttac ctgccaaacg ccaattattg
     9241 gctgggtcag ttaaactaca acaagggtaa aaaagatgat gcggcgtact attttgcttc
     9301 ggtagtgaaa aactatccga agtcaccaaa ggctgctgat gcgatgttta aagtcggcgt
     9361 catcatgcag gacaaaggtg acaccgcaaa agcgaaagcc gtataccagc aggttatcag
     9421 taaataccct ggtactgatg gcgctaaaca ggcacaaaaa cgtctgaacg cgatgtaatg
     9481 cataacacac gaccagaagt cgcattattt ctggtcgtgt cgtgcgaatc ataagcagtt
     9541 gagtgatctg catcgaaatt tttgttgcgc tcaagtctga aatcagtaat atatgccgcc
     9601 gttgccacgg gatatcaaac aaaccgaaag caacgaaaaa gtgggtcgtt agctcagttg
     9661 gtagagcagt tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccaat
     9721 cgctaaggtg gaagcggtag taaaacgtga aggataacgt tgcatcagca acggcccgaa
     9781 gggcgagacg aagtcgagtc atcctgcacg acccaccact aacatagtta gttgtagtat
     9841 ccagcgtagt attgggtgat tagctcagct gggagagcat ctcccttaca aggagggggt
     9901 cggcggttcg atcccgtcat cacccaccac cgggtcgtta gctcagttgg tagagcagtt
     9961 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccagtt ttagcatcga
    10021 agacagatgt taagcgtgta ggataacgtt gcgtcagcaa cggcccgtag ggcgagcgaa
    10081 gcgagtcatc ctgcacgacc caccactaat tacggtgggt tcggtggaag tagtttgtag
    10141 tatccagcgc agtatcgggt gattagctca gctgggagag cacctccctt acaaggaggg
    10201 ggtcggcggt tcgatcccgt catcacccac cactcgggtc gttagctcag ttggtagagc
    10261 agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aattttaaca
    10321 tcaaactcag atgttaagcg tgaaggataa cgttgcgcca gcaacggccc gtagggcaag
    10381 cgaagcgagt catcctgcac gacacaccaa tcttaaagat tggccccgag tagaaatctt
    10441 tcaggtaaca cccgtatggg tcgttagctc agttggtaga gcagttgact tttaatcaat
    10501 tggtcgcagg ttcgaatcct gcacgaccca ccatcctgaa tgattaaagc agcataatcc
    10561 cgcaaggggt cgttagctca gttggtagag cagttgactt ttaatcaatt ggtcgcaggt
    10621 tcgaatcctg cacgacccac caatgtaaaa aagcgcccta aaggcgcttt tttgctatct
    10681 gcgatactta aagattcgaa cctgcagcag gtttgagttg agcgcagcga aacaacggag
    10741 ccgctcgcgg cgacggcccg aagggctagc gaagcgagtc atcctgcacg acccaccaat
    10801 gtaaaaaagc gccctaaagg cgcttttttg ctattcaggc atcctcaatt tcactttgta
    10861 aacctgatga catcgtcaga gcttactgtg caagcaactc tacgtcggtg gaattaggcg
    10921 taattcgagt gacttttccc caccattcga ctatcttgtt tagcatataa aacaaattac
    10981 accgataaca gcgaatatta cgctaatgtc ggttttaacg ttaagcctgt aaaacgagat
    11041 ggtaagatga gcgtaatgtt tgatccagac acggcgattt atcctttccc cccgaagccg
    11101 acgccgttaa gcattgatga aaaagcgtat taccgcgaga agataaaacg tctgctaaaa
    11161 gaacgtaatg cggtgatggt tgcccactac tataccgatc ccgaaattca acaactggca
    11221 gaagaaaccg gtggctgtat ttctgattct ctggaaatgg cgcgcttcgg tgcaaagcat
    11281 cccgcttcta ctttgttagt cgctggggtg agatttatgg gagaaaccgc caaaattctc
    11341 agtccggaaa aaacaattct gatgccgaca cttcaggctg aatgttcact ggatctcggc
    11401 tgccctgttg aagaatttaa cgcattttgc gatgcccatc ccgatcgtac tgtcgtcgtc
    11461 tacgccaaca cttctgctgc ggtaaaagcg cgcgcagatt gggtggtaac ttcaagcatt
    11521 gccgtcgaac ttattgatca tcttgatagt ttgggtgaaa aaatcatctg ggcacccgac
    11581 aaacatctgg ggcgttacgt gcaaaaacag acgggtggag acattctatg ctggcagggt
    11641 gcctgtattg tgcatgatga atttaagact caggcgttaa cccgcttgca agaagaatac
    11701 ccggatgctg ccatactggt gcatccagaa tcaccacaag ctattgtcga tatggcggat
    11761 gcggtcggtt ccaccagtca actgatcgct gctgcgaaaa cattgccaca tcagcggctt
    11821 atcgtggcaa ccgatcgggg cattttctac aaaatgcagc aggcagtgcc agataaagag
    11881 ttactggaag caccaaccgc aggtgagggt gcaacctgcc gcagctgcgc gcattgtccg
    11941 tggatggcca tgaatggcct tcagtccatc gcagaggcat tagaacagga aggaagcaat
    12001 cacgaggttc atgttgatga aaggctgcga gagagggcgc tggtgccgct caatcgtatg
    12061 ctggattttg cggctacact acgtggataa cgaataataa ggcgtaacgt tacgctttgg
    12121 gggaaagatg gattttttta gtgtgcagaa tatcctggta catataccaa taggggcagg
    12181 cggttatgat ctctcatgga tcgaagcggt aggcacgatc gccgggttgc tgtgtattgg
    12241 ccttgccagt ctggagaaga tcagcaacta cttctttggc ctgatcaacg tcaccttgtt
    12301 tggcattatt ttctttcaga ttcagctgta tgccagcctg ctattacagg tgtttttctt
    12361 tgccgcgaat atttacggtt ggtatgcgtg gtcgcgacaa accagtcaga acgaggcgga
    12421 gttgaaaatt cgctggttgc cattgccgaa ggcactcagc tggttggcgg tttgcgttgt
    12481 ttcgattggt ctgatgacgg tatttatcaa tccggtgttt gcatttttga cccgcctggc
    12541 agtcatgatc atgcaagcat taggattaca ggttgtgatg cctgaactgc aaccggacgc
    12601 tttcccgttc tgggattcat gcatgatggt gttatctatc gtggcaatga ttctgatgac
    12661 gcgtaagtat gtggaaaact ggctgttgtg ggtgattatt aacgtgatta gcgtcgttat
    12721 ttttgcactt cagggcgttt acgccatgtc tctggagtac atcatcctga cctttattgc
    12781 gctcaacggc agccggatgt ggatcaacag cgcacgtgaa agaggctcac gcgcgctgtc
    12841 ccattaatgg tgatgatgtg aatggccaga caccccttca ttcaggtggc agtctggccc
    12901 atgacaaggt tgatattcca tctgaatggt ggcgtgctca atctgatagt gatccatcag
    12961 gtagtgttgg atctgatcca acaaggcatc gtgatcgtgt ggcgggatca cctgcacatg
    13021 caacgtcatc accggcttct cgcctaccat ccatacatgt acatggtgca cattgcggac
    13081 ttccgggatt tcccggcaca tacgacgctt cagttcagcg atatccagcg ataccggtgc
    13141 accttcaagt aattcattca cgctatcttt caacagtcgc cacgcgctac gcagaaccag
    13201 aagcgacacc agtatcgaga gaatggggtc agcaggtgtc cagccagtcc agataataat
    13261 caacgcggca ataatagccc caaccgaacc cagcaggtcc ccgagcacat gcagtgccgc
    13321 tgctcgtacg ttgaggtttt tctcttcgct gccgtgatga agtaaccaga aagaaagtat
    13381 atttgccagc aacccagcca cggcaattgc catcatcatg ccgccctcga ccggacgcgg
    13441 cgtgcggaag cgttctatcg cctcccagac aatcaaaatg gtaattacca cgagggctat
    13501 ggcattcaca aacgccgcga gcgtggtcag tcttagccaa ccgaaagtgt ggcgaatggt
    13561 gggaggacga cgtgaaaatt gcacggcgag tagggcgaaa agcagggcgg cagtatcggt
    13621 caacatatga cccgcatcgg ccagcaatgc cagagaacca gaaagaaaac caccaacgac
    13681 ttctaccagc ataaacccgg cagtcacgcc gaaagcatac aacaagcgac gagcattatt
    13741 atcttctggc aggtgtgagg acgtgtgtga gtgtgagtgc gccatgacga gtcatccctt
    13801 aattattctc acttaacttt atgacatcat actgctttta gaagtgaaaa attaaaaggg
    13861 agagactccg ctctcccatt attggctatt ttgcagggtt actgcgtggt accgtcggtt
    13921 ttggtatcga catcattatt gatgccatca ccggtttgta cctttttatt gatatccgga
    13981 cagcgaccat ctttgcacat ggtgttcttg tgctcttcat ctttggtcat tccgtcattg
    14041 ttcattgaag aaccatccga atgcagcatt gtgccaccag aaccggtatt taccccgtta
    14101 ttgtcgacgt tatttggcgc gacattttca cgggcgtcag gggctacctg gcccgcatca
    14161 gctgcggcgt ttgcctggcc gttattagtt tgcgctccgc tatcggcagc cagtgcggca
    14221 ccgctggcaa ggcttagagt ggcagtcaga aataatgtgg ccagttttgt cattttcata
    14281 ggatgctcct gttatggtcg ttatgtcgga taacctcttc caacagtgca tttgcaggtg
    14341 aatataaggc attggtttaa gatttcagcc aggttatgaa acgcagcaga gaatcttgaa
    14401 ataattaaca aacaaaggag ttacagttag aaattgtagg agagatctcg tttttcgcga
    14461 caatctggcg tttttcttgc taattccagg attaatccgt tcatagtgta aaaccccgtt
    14521 tacacattct gacggaagat atagattgga agtattgcat tcactaagat aagtatggca
    14581 acactggaac agacatgaat tatcagaacg acgatttacg catcaaagaa atcaaagagt
    14641 tacttcctcc tgtcgcattg ctggaaaaat tccccgctac tgaaaatgcc gcgaatacgg
    14701 ttgcccatgc ccgaaaagcg atccataaga tcctgaaagg taatgatgat cgcctgttgg
    14761 tggtgattgg cccatgctca attcatgatc ctgtcgcagc aaaagagtat gccactcgct
    14821 tgctggcgct gcgtgaagag ctgaaagatg agctggaaat cgtaatgcgc gtctattttg
    14881 aaaagccgcg taccacggtg ggctggaaag ggctgattaa cgatccgcat atggataaca
    14941 gcttccagat caacgacggt ctgcgtatag cccgtaaatt gctgcttgat attaacgaca
    15001 gcggtctgcc agcggcgggt gagtttctcg atatgatcac cccacaatat ctcgctgacc
    15061 tgatgagctg gggcgcaatt ggtgcacgta ccaccgaatc gcaggtgcac cgcgaactgg
    15121 cgtctggtct ttcttgtccg gtaggtttta aaaatggcac agacggtacg attaaagtgg
    15181 ctatcgatgc cattaatgcc gccggtgcgc cgcactgctt cctgtccgta actaaatggg
    15241 ggcattcggc gattgtgaat accagcggta acggtgattg ccatatcatt ctgcgcggcg
    15301 gtaaagagcc taactacagc gcgaagcacg ttgctgaagt gaaagaaggg ctgaacaaag
    15361 caggcctgcc agcacaggtg atgatcgatt tcagccatgc taactcgtcc aaacaattca
    15421 aaaagcagat ggatgtgtgt gctgacgttt gccagcagat tgccggtggc gaaacagcca
    15481 ttattggcgt gatggtggaa agccatctgg tggaaggcaa tcagagcctc gagagcgggg
    15541 agccgctggc ctacggtaag agcatcaccg atgcctgcat cggctgggaa gataccgatg
    15601 ctctgttacg tcaactggcg aatgcagtaa aagcgcgtcg cgggtaaggt ttaattgtcg
    15661 gatgcgccgt cagagtggcg tatccgatga atcaccacag gcctgataag acgcgcagcg
    15721 tcgcatcagg caatgtgctc cattgttagc aacaaaaaag ccggctcact tgcagtcggc
    15781 tttctcattt ttaaacgaat gacgtttact tcgctttacc ctggttagca accgccgctg
    15841 ctttcgctgc gatctcgtca gcattaccca gataatagcg tttcagcggt ttgaaattct
    15901 cgtcgaactc atacaccagc ggcacgccgg tcgggatatt cagctcaaga atctcttctt
    15961 cactcatgtt atcaagatat ttcaccagcg cacgtaaaga gttaccgtgt gcagcgatga
    16021 tcacgcgctc accgctcttc atacgcggta gaatagtttc attccagtaa gggatcacgc
    16081 ggtcaatggt cagcgccagg ctttccgtca gcggcagttc tttctcgctc agcttcgcgt
    16141 aacgtggatc gtgacccgga taacgctcat catctttagt cagttccggc ggagtgactg
    16201 caaaaccacg acgccactgt ttcacctgct cgtcgccata cttttcagcg gtttctgctt
    16261 tgttcagacc ctgcaacgca ccgtagtgac gttcgttcag tttccaggat ttctcaacgg
    16321 gcagccatgc ctgatccagt tcgtccagca cattccacag ggtatggata gcgcgtttca
    16381 gcacagaagt gtaagcaaag tcaaagctgt aaccttcctc tttcagcagc ttacctgctg
    16441 cttttgcttc gcttacgcct ttctcagaca gatccacgtc gtaccaaccg gtgaaacggt
    16501 tttctttgtt ccactgactt tcgccatgac gaaccagaac cagcttagtt acagccatat
    16561 acttactcct caaatcatct tttaatgata ataattctca ttatattgcc gtgacgaagc
    16621 aacagcaatg cttacgcata accatagcga aaatagtggc gcagtgtaag gttgttgtga
    16681 atattgagtt gcaaatatgt cggtgtttgc tggtgatttg aacaatatga agataaagcc
    16741 ctcatgacga gggcttaaca ttactcagca ataaactgat attccgtcag gctggaatac
    16801 tcttcgccag gacgcaggaa gcagtccggt tgcggccatt cagggtggtt cgggctgtcc
    16861 ggcagaaact cgctttccaa cgccagccct tgccagtcgg cgtaaggttc ggttccccgc
    16921 gacggtgtgc cgccgaggaa gttgccggag tagaattgaa gagccggagc ggtggtgtag
    16981 accttcagct gcaatttttc atctgctgac cagacatgcg ccgccacttt cttgccatcg
    17041 cctttggcct gtaacaagaa tgcgtgatcg taacctttca ctttgcgctg atcgtcgtcg
    17101 gcaagaaact cactggcgat gattttggcg ctgcggaaat caaaagacgt tccggcgaca
    17161 gatttcaagc cgtcgtgcgg aatgccgcct tcatcaaccg gaagatattc gtccgccaga
    17221 atctgcaact tgtgattgcg cacgtcagac tgctcgccgt caagattgaa atagacgtga
    17281 ttagtcatat tcaccgggca aggtttatca actgtggcgc gataagtaat ggagatacgg
    17341 ttatcgtcgg tcagacgata ttgcaccgtt gcgccgagat tacccgggaa gccctgatcg
    17401 ccatcatctg aactcagggc aaacagcacc tgacgatcgt tctggttcac aatctgccag
    17461 cgacgtttgt cgaacccttc cgggccgccg tgcagctggt taacgccctg acttggcgaa
    17521 agggtcacgg tttcaccgtc aaaggtataa cggctattgg cgatgcggtt ggcataacga
    17581 ccaatagagg cccccagaaa cgcggcctga tcctgatagc attccgggct ggcacagccg
    17641 agcagcgcct cgcggacgct gccatcggaa agcggaatac gggctgaaag taaagtcgca
    17701 ccccagtcca tcagcgtgac taccatccct gcgttgttac gcaaagttaa cagtcggtac
    17761 ggctgaccat cgggtgccag tgcgggagtt tcgttcagca ctgtcctgct ccttgtgatg
    17821 gtttacaaac gtaaaaagtc tctttaatac ctgtttttgc ttcatattgt tcagcgacag
    17881 cttgctgtac ggcaggcacc agctcttccg ggatcaacgc gacgatacag ccgccaaatc
    17941 cgccgccggt catgcgtacg ccacctttgt cgccaatcac agctttgacg atttctacca
    18001 gagtgtcaat ttgcggcacg gtgatttcga aatcatcgcg catagaggca tgagactccg
    18061 ccatcaactc gcccatacgt ttcaggtcgc cctgctccag cgcgctggca gcttcaacgg
    18121 tgcgggcgtt ttcagtcagg atatgccgca cgcgtttcgc cacgattggg tccagctcat
    18181 gtgcaacagc gttgaactct tcaatggtga catcgcgcag ggctggctgc tggaagaaac
    18241 gcgcaccggt ttcgcactgt tcacgacggg tgttgtattc gctgccaacc agggtacgtt
    18301 tgaagttact gttgatgatg acgacagcca cacctttcgg catggaaact gctttggtcc
    18361 ccagtgagcg acagtcaatc agcaaggcat gatctttctt gccgagtgcg gaaattagct
    18421 gatccatgat cccgcagtta cagccaacaa actggttttc tgcttcctga ccgttaagcg
    18481 cgatttgtgc gccgtccagc ggcagataat aaagctgctg caatacggtt ccgaccgcga
    18541 cttccagtga agcggaagaa cttaacccgg caccctgcgg cacattgccg ctgatcacca
    18601 tgtccacacc gccgaagctg ttgttacgca gttgcagatg tttcaccacg ccacgaacgt
    18661 agttcgccca ttgatagttt tcatgcgcga caatgggcgc attgagggaa aactcgtcga
    18721 gctgattttc ataatcggct gccattacgc gaactttacg gtcatcgcgt ggcgcacagc
    18781 tgatcacggt ttgataatca atcgcgcagg gcagaacgaa accgtcgttg tagtcggtgt
    18841 gttcaccaat caaattcacg cggccaggcg cctgaatggt gtgagtggca gggtagccaa
    18901 atgcgttggc aaacagagat tgtgtttttt ctttcagact catttcttac actccggatt
    18961 cgcgaaaatg gatatcgctg actgcgcgca aacgctctgc tgcctgttct gcggtcaggt
    19021 ctcgctgggt ctctgccagc atttcataac caaccataaa tttacgtacg gtggcggagc
    19081 gcagcagagg cggataaaag tgcgcgtgca gctgccagtg ttgattctct tcgccattaa
    19141 atggtgcgcc gtgccagccc atagagtagg ggaaggagca ctggaagagg ttgtcataac
    19201 gactggtcag ctttttcaac gccagcgcca aatcgctgcg ctgggcgtcg gtcaaatcgg
    19261 tgatccgcaa aacgtgggct ttgggcagca gtagcgtttc gaacggccag gcagcccagt
    19321 aaggcacaac ggctaaccag tgttcggttt cgacaacggt acggctaccg tctgccagct
    19381 cgcgctgaac ataatccacc agcattggtg atttctgttc ggcaaaatat tctttttgca
    19441 ggcggtcttc gcgctcagct tcgttaggca ggaagctatt tgcccaaatc tgtccgtgcg
    19501 gatgcgggtt agagcagccc atcgccgcac ctttgttttc aaagacctgc acccacgggt
    19561 acgtttttcc cagctctgcg gtttgctcct gccaggtttt gacgatttcc gtcaatgccg
    19621 caacgctcag ttctggcaac gttttactgt gatccggtga aaagcagatc acccggctgg
    19681 taccgcgcgc gctctggcaa cgcatcaaag gatcgttgct ttctggcgca tctggcgtgt
    19741 cagacatcaa ggccgcaaag tcattagtga aaacgtaagt cctggtgtaa tcggggtttt
    19801 tatcgcctgt cacccgcaca ttacctgcgc agaggaagca atctggatcg tgcgcaggta
    19861 acacctgttt ggctggcgtt tcctgcgccc cctgccaggg gcgcttagcg cggtgcggtg
    19921 aaaccagaat ccattgcccg gtgagcgggt tgtagcggcg atgtggatga tcaacgggat
    19981 taaattgcgt catggtcgtt ccttaatcgg gatatccctg tggatggcgt gactgccagt
    20041 gccaggtgtc ctgcgccatt tcatcgagtg tgcgcgttac gcgccagttc agttcacggt
    20101 cggctttgct ggcgtccgcc cagtaggccg gaaggtcgcc ctcgcgacgc ggtgcaaaat
    20161 gataattaac cggtttgccg caggctttgc tgaaggcatt aaccacgtcc agcacgctgt
    20221 tgcctacgcc agcgccgagg ttgtagatgt gtacgcctgg cttgttcgcc agtttttcca
    20281 tcgccacgac gtgaccgtcc gccagatcca ttacgtggat gtaatcgcgt acgccagtac
    20341 catcttcggt cggataatcg ttaccaaaaa tcgccagcga gtcgcgacgg cctacagcaa
    20401 cctgggcgat gtatggcatc aggttattcg gaatgccttg cggatcttcg cccatatcgc
    20461 ccgacggatg cgcgccaacc gggttgaagt agcgcagcag ggcaatgctc cagtccggct
    20521 gggctttttg cagatcggtg aggatctgtt ccaccatcag cttgcttttg ccgtaagggc
    20581 tttgtggtgt gccggtcggg aagctttcaa cgtatggaat tttgggctga tcgccataaa
    20641 cggtggcgga ggagctaaaa ataaagtttt tgacgttagc ggcgcgcatg gcgctaatca
    20701 ggcgcagagt gccgttgaca ttgttgtcgt aatattccag cggtttttgt accgattcgc
    20761 cgacggcttt cagcccggcg aagtggatca cggtgtcgat agcgtgatcg tgcagtatct
    20821 cggtcatcaa cgcttcgtta cgaatatcgc cttcaacaaa cgttggatgt ttgccgccta
    20881 aacgctcgat aacaggcagt acgctgcgct tactgttaca gaggttatca agaatgatga
    20941 catcatgacc gttttgcagt aattgcacac aggtatgact tccaatgtaa ccgctaccac
    21001 cggtaaccag aactctcata attcgctcca ttaggcttat ggtatgaaat aaccatagca
    21061 taacaaagat gcgaaaagtg tgacatggaa taaattagtg gaatcgttta cacaagaatt
    21121 tagccgtttt ttatgcgcga ttaagtgatt ataaaacaga gggtttatga atgattgcgc
    21181 tttttatctg aaaaaagaag cggtttcatg cctgcatgcg tcgaaccgtt ggccggagag
    21241 ggcgcttagg ccgcctccgg caaggtcagc actaccgact caatatattt ttgtcagcac
    21301 atagcgatag agtccaccgt ccggcacgaa ctcaaggcga tgggtaatac aggcaggcgc
    21361 atcttcagcg tggtgcgaaa caaacaacaa ttgcgtttca ccttcgctaa tcagcacatc
    21421 aacaaaacgg cggataagct ggcgattgag cggatcaagc ccctgtagtg gttcatcgag
    21481 aataagcaac gtcggatgtt tcaccagtgc gcggacgatc agcgccagac gctgctgtcc
    21541 ccaggaaaga ctatggaacg gagcgtcagc cgtacgttta tcaatgccga gaatatccag
    21601 ccactgctgc accagttttt gctggcgatc cgaaacggcc tgataaatgc caatcgaatc
    21661 aaaatagcca gaaagaatca cattacgcac ggtagtgctg acccggtaat ccagatgcaa
    21721 actactgctg acgtaaccga tatgcttttt gatatcccag atggtttcgc cgctgccgcg
    21781 acgtcgtccg aaaagcgtca aatcgttgct gtaaccttgc ggatgatcgc cagtaaccag
    21841 gcttaataac gtcgattttc ctgcaccatt tggcccgaca atttgccagt gttcacctgg
    21901 attcacctgc cagctaaggt tattaagaat ggggcgatcg ttataagaaa ccacgccatt
    21961 gttcagcaca atgcgcggtt cgttggcggg taaggcgtga cgtgctgaag gttcatccgg
    22021 ctccggcagt tgcacacctt caagctgttc actatgcgcc agttgcgcga cgagtgcttg
    22081 ttggagcagt tcctctttag cgccagtttc cgctaacgtg caatccgcca gcacgccagc
    22141 aaactggaca aactccggga tctcatcgaa gcgattgagc accagtacca gagtaatacc
    22201 ggactgatgt aacgaggcga gtcgctcagc cagctgctga cgtgaggcaa catccaggcc
    22261 atcgaacggc tcatcaagaa tcaacaagtc aggctccgac atcagcgcct gacacagcag
    22321 ggtttttcgc gtctcgccag tggaaaggta tttaaagcgt cggtcgagga gggcggtaat
    22381 accgaactgc tgcgccagtt gcatgcaacg cggtgcatcc tttacttcat cctgaatgat
    22441 ctcagccgta gtgcgtccgg tgtcatcttc gccagggccg agcatatcgg tgttattccg
    22501 ctgccattcg tcgctgacga gtttttgcaa ttgctcgaag gagagacgag tgatgtggga
    22561 aaactggctt tgccgttcac ctttcaaaag cggaagttcc cccgccagcg cgcgggccag
    22621 ggccgatttc ccgcttccat tcgaaccgac aaacgcccaa ctatcacccg cgtttaacgt
    22681 tagctgaggc aattgcagcg tttttgtgtc gctaagacga aacgtgcctt gcaaaatttg
    22741 caacgatgac attttatatc ccattttgtg cagcgattac tgacagggat acgtgtttca
    22801 taacaaattg tcaacacgct tagcacagcg tggcgataat cacgctgtcg gcattaaagt
    22861 aggccgtgac attctgccct tgctgaagag aagtcgcttc atttaccggc actgtggcgc
    22921 acagtgtttg cccgtcgggt agcgccatta atacttcgca ctgctctgcg ccgcgctcaa
    22981 tatgactaat aatacccggt aattggttgt cggcgttttg tgcgaccgcc tcgtcctgag
    23041 taatacctac ccacggcgct tttagcaata tcaacacttc tttgccttca tccagcccca
    23101 gacgcgcgcc gctttgtgcg gtaattgcga ctttcaggcg tgtttttccg tcagccagta
    23161 agacatcaac atgctgttga acgtcatcat gatcgcgggc ggtgatggtg ccgaaccact
    23221 ggttacgggc gctggtttgc agtgaaaaac gtgagatcgc ggccagcagg ctgttcagcg
    23281 gcagggcgtc atcgtcactt aacacatcaa aggctttttg ctggatttgc gccagtaagt
    23341 catagagctg aatcagtcgc tgaccatagc gggtcagtac tgcgccgccg ccacctttac
    23401 cgcctgttgc gcgctcgacc agaatatgct cacttaactg attcatctcg ttaatggcat
    23461 cccaggcgct tttatagctg ataccggcat ctttcgctcc ctggctaatg gaaccggaaa
    23521 gcgcaatgtg ttttagtagc gaaatccggc gcgggtcggc gaataatttt tgttggagct
    23581 taagggtgag aaggatttcg gcctgcataa caatgtcctg gcaaaagtct tattgtgacg
    23641 gaaaacgaac gccacgcaaa gctgaccgca caaaagggga gtgcttttct gtgcttagcg
    23701 gttagaatag tctcatgact atatctggag ttgaccatgt tagagttatt aaaaagtctg
    23761 gtattcgccg taatcatggt acctgtcgtg atggccatca tcctgggtct gatttacggt
    23821 cttggtgaag tattcaacat cttttctggt gttggtaaaa aagaccagcc cggacaaaat
    23881 cattgattcc ctgaatgccc gcttagtcgg gcatttcttt ttctcaactt cctgcttttc
    23941 cagccgatat tttttcttat ctacctcaca aaggttagca ataactgctg ggaaaattct
    24001 gagctagccg ttatattgtc gcctacataa cgttacatta aggggttacc aatggctcgt
    24061 aaatggttga acttgtttgc cggggcggca ctctctttcg ctgttgctgg caatacactg
    24121 gcagatgaag ggaaaatcac ggtgttcgcc gccgcatcac tgactaacgc aatgcaggac
    24181 attgctacgc agtataaaaa agaaaaaggc gtggatgtgg tttcttcttt cgcttcgtca
    24241 tctactctgg cccgtcagat tgaagcggga gcgcctgcgg atctgtttat ctctgccgat
    24301 cagaaatgga tggattatgc ggttgataaa aaagcgatcg atacggcttc gcgccagaca
    24361 ctgctcggca atagcctggt cgttgtagca ccgaaagcca gcgagcagaa agatttcacc
    24421 atcgacagca aaaccaactg gacttcgctg ctgaatggcg gtcgcctcgc ggttggcgat
    24481 ccggaacatg tccctgctgg catttatgcg aaagaagcac tgcaaaaact gggcgcatgg
    24541 gatacgctct ccccgaaact ggctccggcg gaagatgttc gtggcgcgct ggcgttggtc
    24601 gaacgcaatg aagcgccgtt gggcattgtt tacggttctg acgcagttgc cagcaaaggg
    24661 gtaaaagtgg ttgccacctt cccggaagat tcacataaaa aagtggaata tccggttgct
    24721 gttgtggaag agcataacaa tgcgacagtg aaagcatttt atgattattt gaaagggccg
    24781 caggctgcgg aaatctttaa acgttacgga tttacaacca agtaatgata ctgaccgatc
    24841 cagaatggca ggcagtttta ttaagcctga aagtttcttc cctggctgtg ctgtttagcc
    24901 tgccgtttgg gatctttttt gcctggttac tggtgcgttg cacgtttccg ggcaaagctc
    24961 tgctcgacag cgtactgcat ctaccgctgg tgttaccgcc cgtggtcgtc ggttacttat
    25021 tattagtttc gatgggacgg cgcggattta tcggtgaacg tctgtatgac tggtttggta
    25081 ttaccttcgc ctttagctgg cgcggcgcgg ttctcgctgc cgccgtcatg tcgtttccgc
    25141 tgatggtgcg ggcaattcgt ctggcgctgg aaggggttga tgtcaaactc gaacaggccg
    25201 caagaacact gggggccggg cgctggcgcg ttttctttac tatcacgtta ccgctgacct
    25261 taccgggaat tattgttggt acggtactgg cttttgctcg ttctctcggt gagtttggtg
    25321 caaccatcac ctttgtgtcg aacattcctg gtgaaacgcg aaccattcct tctgccatgt
    25381 ataccctgat ccagaccccc ggcggggaaa gtggcgcggc gagactgtgc attatttcta
    25441 ttgcgctggc gatgatctcc ctgttgattt cagaatggct ggccagaatc agccgtgaac
    25501 gggcggggcg ctaatcatgc tggaactgaa tttttcccag accttgggca accattgcct
    25561 gaccattaat gaaacgctgc ccgccaatgg catcactgct atctttggcg tctccggtgc
    25621 cggaaaaact tcgctgatta acgccatcag tggactgacg cgcccgcaaa aagggaggat
    25681 tgtcctcaat ggtcgggtac taaatgatgc cgaaaaaggt atctgcctga cgccagaaaa
    25741 gcgtcgcgtt ggctatgttt ttcaggatgc gcggctgttt ccgcattaca aagtgcgtgg
    25801 caatctgcgc tacggcatgg cgaaaagcat ggtcgatcag ttcgataagc tggtggcgct
    25861 tttaggcatt gaaccgttgc ttgaccgttt accaggcagc ctgtccggag gcgaaaaaca
    25921 gcgcgtagcg attgggcggg ctttgctgac ggcaccagaa ttgctgttgc tggatgaacc
    25981 gctggcgtca ctggatattc cgcgtaaacg cgaactgttg ccttatctgc aacgtctgac
    26041 gcgggaaatc aacatcccga tgttgtatgt cagccattcg ctggatgaga tcctccatct
    26101 ggcagacaga gtgatggtac tggaaaacgg tcaggtgaaa gcctttggcg cactggaaga
    26161 agtctggggt agtagcgtga tgaatccgtg gctgccgaaa gagcaacaaa gtagcattct
    26221 gaaagtgacg gtacttgagc atcatccgca ttacgcgatg actgcgctgg cgctgggcga
    26281 tcagcatttg tgggtcaata agctggacga accgctgcaa gctgcgctac gtatccgcat
    26341 tcaggcttcc gatgtttctc tggttttaca accgccgcag caaaccagca ttcgtaacgt
    26401 attgcgggca aaagttgtta atagttatga cgacaacggc caggtggaag tggaactgga
    26461 agtcggaggt aaaacgctgt gggcgcgtat cagcccgtgg gccagggatg aactggtgat
    26521 caaacctggc ctgtggctgt acgcgcaaat taaaagtgtg tcgataaccg cctgattaaa
    26581 tcaggtggct ataaatgaac tgggcaatgc tgtcggtggt gttatcacca atcacaatgt
    26641 tggcgcgcgc ttttaccgcg tcatcggcgt tgcccatcgc cacgcctgta ccagcggcct
    26701 ccagcatact gatatcatta aagttatcgc cgaacgccac gacgttttcc atcgaccagc
    26761 cttgcgcctc aacccatttc gtcaaacgtt tacctttgct gttgccgccg cgtgcaatat
    26821 caacctgatc gtgccaggac cattcacact ccagtcccag ttcatgttcg acatgcttac
    26881 caaaatgctg caattgcggc aggtcatcgt gcgtcagggc gaacttccat acggcgttaa
    26941 cttgttgcgc cgtttcagcc agagaagcga cttgtgtgaa agtcggacgc tgttccggcg
    27001 gcagggtttg cgcccagtta gacgtgcgaa tgacatgccc ggtcgggtgc tcatagacca
    27061 ttgcatcatc cacatacatc agaccgtgaa tgtggtgttc attcagcatc tcaatgagtt
    27121 gcagggcttt attaacgggc attgggtccg cttccagcac ggtttttgca tgataatcat
    27181 acaaataggt gccattacag caaatagcag gtgtatccag cgccagcgcc tgataaaaag
    27241 gatgaatagc gacgtgatgg cgacctgtga cgatgattaa ttgatagcct gcttcgcgag
    27301 cgcgggccag ggcttctatc gatgaaggaa gcagggtctt tttcggggtc aataaggtgc
    27361 cgtctaagtc gagagcaatc acgcgtgtgg tcatggcgta ttccagatta aggttaagaa
    27421 ttttcgtctg cgcgaatggt acaccgatac cactatcgga caaaattctg cgttttaatt
    27481 cagcattcac cgccaaaagc gactaatttt agctgttaca gtcagttgct aaatgcaaag
    27541 gagcattcat gaagcaaaca gtttatatcg ccagccctga gagccagcaa attcacgtct
    27601 ggaacctgaa tcatgaaggc gcactgacgc tgacacaggt tgtcgatgtg ccggggcagg
    27661 tgcagccgat ggtggtcagc ccggacaaac gttatctcta tgttggtgtt cgccctgagt
    27721 ttcgcgtcct ggcgtatagt atcgccccgg acgatggcgc actgaccttt gccgcagagt
    27781 ctgcgctgcc gggtagtccg acgcatattt ccaccgatca ccaggggcag tttgtctttg
    27841 taggttctta caatgcgggt aacgtgagcg taacgcgtct ggaagatggc ctgccagtgg
    27901 gcgtcgtcga tgtggtcgag gggctggacg gttgccattc cgccaatatc tcaccggaca
    27961 accgtacgct gtgggttccg gcattaaagc aggatcgcat ttgcctgttt acggtcagcg
    28021 atgatggtca tctcgtggcg caggaccctg cggaagtgac caccgttgaa ggggccggcc
    28081 cgcgtcatat ggtattccat ccaaacgaac aatatgcgta ttgcgtcaat gagttaaaca
    28141 gctcagtgga tgtctgggaa ctgaaagatc cgcacggtaa tatcgaatgt gtccagacgc
    28201 tggatatgat gccggaaaac ttctccgaca cccgttgggc ggctgatatt catatcaccc
    28261 cggatggtcg ccatttatac gcctgcgacc gtaccgccag cctgattacc gttttcagcg
    28321 tttcggaaga tggcagcgtg ttgagtaaag aaggcttcca gccaacggaa acccagccgc
    28381 gcggcttcaa tgttgatcac agcggcaagt atctgattgc cgccgggcaa aaatctcacc
    28441 acatctcggt atacgaaatt gttggcgagc agggactact gcatgaaaaa ggccgttatg
    28501 cggtcgggca gggaccaatg tgggtggtgg ttaacgcaca ctaaccgctg atttaaccgg
    28561 cgcagtctct cctgcgccgg tgtattaacc tatctcctgt aacgcatgtc tctggcgttc
    28621 gacgatattg gtccacaaat tgtctttatc gtcagtccac aaattaatca gcaaggcaaa
    28681 aaagcgttct gctgccggag aaagcacggc atctttgcgg cgaataatcc ccaatgtccg
    28741 acgtataacg ggttccacca gtggaatacc aataatcgaa gaatagggcg catggggcat
    28801 tgccagacca ggaagcgccg aaatccccag tcccgcctct accagtccta atgacgtcga
    28861 aagatgacgc acttcgtaaa accagtccag cttccagggc ttgtcggcta gctgttgttc
    28921 tatcaacaat cggttgccgc tggaggaacg tacgccaatc attttgtaac ccactaactc
    28981 ctgccattct acaagctgct ttttggccaa tggatggtca cgccgacagg ccagcacgaa
    29041 tggctcgttg accagtgggg taaaatcaat ggatgaattt gtgacgttgt tcatgtttat
    29101 gccaaagtct gattcgttac acagcaccga ttccatgcaa ttattcgtgc cttgttccag
    29161 aatccgcact ttaatattgg gatacagctc attaaattta ccgattgcca gcggtaaaaa
    29221 ataaaatact gcggtcggaa tacacgctaa cgtcaccata ccacgatgat aagcattcat
    29281 atcacgaata ttaaaaagcg tttcatcgaa tttctttatt aattcccgcg cctccgggag
    29341 caaccttttt cctgcttttg ttaacgtaac cttgcgagtt gtacgctcaa atagttgaat
    29401 atgtaaatct tcctccatct tttttattct tcgcgttaat gcaggttgcg taatattgag
    29461 taatttagca gcattgttaa atgaactgga ctctgccagt atgacaaatg ccttcatact
    29521 tgataattca tgcttcatta ttactccgga aaatggaagc gacgattttg ggtggctggt
    29581 cgttaaaaat tttaactgca tttagccaac ttaaattaat gaaaaaatgt tattaatcgt
    29641 tgagctaaag tcattagaga tgctttgtcc ttaatgtaac catatcgcaa taagttatgt
    29701 ttttaaattg agggcattat tatgaaaaaa ataccctgcg tgatgatgcg aggtggaacc
    29761 tcgaggggcg cgttcctgtt agcggaacat ttacccgaag atcaaacgca gcgcgataaa
    29821 atattgatgg caattatggg ttccggtaac gatctggaaa ttgacggtat tggcggcggt
    29881 aatccactga ccagtaaagt cgccattatt agccgttcca gcgatccgcg tgctgatgtt
    29941 gattatctgt ttgctcaagt tatcgtccat gagcaacgtg tcgataccac gcctaactgc
    30001 ggcaatatgc tttctggcgt tggggcattc gccattgaaa atggtttgat tgcagcgaca
    30061 tcaccggtca cccgtgtgcg tatccgcaac gtcaatacgg gtacgttcat cgaagctgat
    30121 gtgcaaacgc caaatggtgt tgtcgagtac gagggtagtg ccagaattga cggcgtaccg
    30181 ggtactgccg caccggttgc gctcactttc ctgaatgccg ctggaaccaa aaccggaaaa
    30241 gttttcccga ctgataatca gattgattat tttgacgatg tcccggtgac ctgtatcgat
    30301 atggcgatgc cagtcgtcat tattccggct gaatatctgg gaaaaacagg ttatgaatta
    30361 ccggcggaac tggatgccga caaagcatta ttagcccgca ttgaatctat ccgtctacaa
    30421 gcgggtaaag caatgggctt aggtgatgtc agtaatatgg ttatccctaa acctgtgctt
    30481 atttctccag cgcagaaagg cggggcaatt aatgtgcgtt attttatgcc gcattcttgc
    30541 catcgcgcgc tggcgataac cggtgctatt gctatttcca gtagttgtgc attggaaggc
    30601 accgtcaccc gacaaatcgt cccttctgta ggatacggca atatcaatat tgaacacccc
    30661 agtggtgcgc tcgacgttca tttaagtaat gaaggtcagg atgccacgac gttacgcgca
    30721 tctgttattc ggacgaccag aaaaatattt tccggtgaag tttatcttcc ctgaaaaaat
    30781 tcgttgtcaa gataaggata atcaataaag gacttctgta tgagtcatac agaaagaaca
    30841 ggattttaaa tgaataagaa atcgttatgg aagctaattc tgatattagc gatcccatgt
    30901 attattggtt ttatgccagc tccggcagga ttaagcgaac tggcgtgggt gctttttggt
    30961 atttacctgg cggccattgt ggggctggtt atcaagcctt tcccggaacc tgtcgtactg
    31021 ttaattgccg ttgctgcctc catggtggtg gtcggtaact tatccgacgg tgcgtttaag
    31081 accaccgccg tattaagcgg ttactcttca ggtactacct ggctggtgtt ctcggcgttt
    31141 accttaagcg ccgcatttgt gaccaccggt ttaggtaaac gtattgccta tctgctgatt
    31201 ggtaaaatcg gtaacaccac gctgggtctg ggttacgtta cggtattcct cgatctggta
    31261 ctggctccgg caacaccgtc taacaccgcg cgtgcgggcg gtattgtgtt accgatcatc
    31321 aacagcgtgg cggtggcttt ggggtccgaa ccggaaaaaa gtccgcgtcg tgtcggacat
    31381 tacctgatga tgtccattta catggtcacc aaaaccacca gctatatgtt ctttaccgca
    31441 atggcgggga acattctggc gctgaaaatg atcaacgaca ttctgcacct gcaaattagc
    31501 tggggtggat gggcactggc cgccggattg ccgggcatca ttatgctgct ggtcacccca
    31561 ctggtgattt ataccatgta tccgccagaa attaagaagg tggataataa aaccatcgct
    31621 aaagcgggcc ttgccgaact gggaccgatg aaaatccgcg aaaaaatgct gctcggtgtc
    31681 tttgtgctgg cgctactggg ctggattttc agtaagtctc tgggggttga tgaatccact
    31741 gtggcaatcg ttgttatggc gaccatgctg ctgctgggta tcgttacctg ggaagacgtg
    31801 gttaaaaata aaggcggctg gaatacctta atctggtacg gcggtattat cggattaagc
    31861 tccttattat cgaaagttaa attcttcgaa tggttagctg aagtctttaa aaataacctg
    31921 gcatttgatg gtcacggtaa cgttgctttc ttcgttatta ttttcctcag cattatcgtg
    31981 cgttatttct tcgcttccgg tagtgcctat atcgttgcta tgttaccggt atttgccatg
    32041 ctggcgaacg tctccggcgc accgttaatg ttaaccgcgc tggcactgtt gttctccaac
    32101 tcctatggcg gcatggttac tcactatggc ggcgcggcag gtccggtcat ctttggcgtg
    32161 ggttataacg atattaaatc ctggtggttg gtcggtgcgg tactgacgat attaaccttc
    32221 ctggtgcata tcaccctcgg cgtgtggtgg tggaatatgc tgatcggctg gaacatgctg
    32281 taaatattct cgtcatactt caagttgcat gtgctgcgtc tgcgttcgct caccccagtc
    32341 acttacttat gtaagctcct ggggattcac tcgcttgtcg ccttcctgca actcgaatta
    32401 tttagagtat atccatttat tatctttctg cgcacttcac ggtgcgcaga tatctggagc
    32461 atttgatgat caagttatct gaaaaaggcg tgtttctcgc cagtaataac gaaataattg
    32521 ccgaagaaca tttcaccggc gaaattaaaa aagaagaagc caaaaaaggc actattgcct
    32581 ggtctattct ctcttcgcat aatacgtccg gaaatatgga taaacttaaa attaagtttg
    32641 attcattagc ctctcacgat attacctttg ttggtattgt acagaccgct aaagcgtccg
    32701 gtatggaacg tttcccgctg ccgtatgtgc tgaccaactg ccataactca ctctgcgccg
    32761 tcggcggcac tattaacggt gatgaccatg tttttggttt atcggcggcc cagcgttatg
    32821 gcggtatttt tgtgcctccg catattgcgg tcatccatca atatatgcgt gagatgatgg
    32881 caggcggcgg caaaatgatc ctcgggtcag acagccacac ccgttacggt gcattaggga
    32941 caatggcagt cggtgagggc ggcggtgagt tggtaaaaca gctgcttaat gacacctggg
    33001 atatcgacta tccgggcgtg gttgcggtgc atctgaccgg aaaacctgcg ccgtatgtgg
    33061 ggccacagga tgtggcgctg gctatcattg gcgcggtgtt caaaaacggt tacgtcaaaa
    33121 acaaagtcat ggagttcgtt ggaccgggcg ttagcgcgct ctctaccgat ttccgtaaca
    33181 gcgttgacgt gatgaccacg gaaaccacct gtttaagctc cgtctggcaa actgatgaag
    33241 aagtgcataa ctggctggcg ctgcacggtc gcggccagga ttactgccag cttaaccctc
    33301 aaccgatggc gtactacgat ggctgcatca gcgttgattt aagcgccatc aaaccaatga
    33361 ttgcgctgcc gttccacccg agcaacgtgt atgaaatcga cacactgaac cagaacctga
    33421 ccgacattct gcgtgagatt gaaattgagt ccgaacgcgt ggcgcacggt aaagccaaac
    33481 tctcgctgct ggataaagtg gaaaatggtc gcctgaaagt gcagcagggg attatcgcgg
    33541 gctgttctgg cggtaactac gaaaacgtca tcgcggcggc gaatgcactg cgcggtcaat
    33601 cctgtggcaa tgacaccttc tcgctggcag tttacccgtc atcacagccg gtgtttatgg
    33661 atctcgccaa aaaaggtgtg gtagcagatt tgattggcgc aggcgcaatc atcagaaccg
    33721 cgttctgcgg cccatgcttt ggcgcgggcg atacgccaat caacaacggt ttgagtattc
    33781 gccacaccac gcgtaacttc ccgaaccgcg aaggctctaa gccagctaat gggcagatgt
    33841 cagcggtggc gttgatggac gctcgttcta tcgctgcgac tgcggcaaac ggtggctatt
    33901 taacctctgc cagcgaactt gattgctggg acaacgtgcc ggagtacgcc ttcgatgtaa
    33961 cgccgtataa aaaccgtgtt tatcagggct ttgtgaaagg ggcaactcag caaccgctga
    34021 tttacggacc gaacattaaa gactggccgg aattgggtgc gctgactgac aatatcgtcc
    34081 tgaaagtgtg ctcgaagatc ctcgacgaag tgaccaccac cgacgaactg attccttccg
    34141 gtgaaacctc ttcttatcgt tcaaatccga ttggtctggc ggagtttacc ctgtcacgcc
    34201 gcgatcccgg ttatgttggc agaagtaaag cgactgctga gctggaaaat cagcgtctgg
    34261 cggggaatgt cagcgagctg acagaggtgt ttgcgcgcat taagcagatt gctggtcagg
    34321 agcatattga tccgctgcaa actgaaattg gcagcatggt atatgcggtg aaaccaggcg
    34381 atggttctgc gcgtgaacag gcggcgagct gccagcgtgt gattggcggt ctggcgaata
    34441 ttgccgaaga gtacgcgact aaacgctacc gttctaacgt catcaactgg gggatgttac
    34501 cgctgcagat ggcggaagtg ccaacctttg aagtggggga ttacatttac atccctggca
    34561 ttaaagcggc gctggataat ccgggtacga cgtttaaagg ttatgtgatc catgaagatg
    34621 cgccggtaac ggaaattacg ctctatatgg aaagtctgac tgctgaagag cgcgagatta
    34681 tcaaggcggg tagtttgatt aacttcaata aaaaccgtca gatgtaaaaa gcgccatgtg
    34741 aatgtaggtc gcattcggca cttattgtcg gatgcgatgc ttgcgcatct tatccgacct
    34801 acgaatcgca tcgaatctgt aggccagata aggcattttc gcagcatccg gcacttattg
    34861 tcggatgcga tgcttgcgca tcttatccga cctacaaatc gcatcgaacc gtaggccgga
    34921 taaggcgttt acgccgcatc cggcaaatag ttaattgctc ttacttcttc gcctctgcaa
    34981 ccactttact acccacgccg cggttattgt attcccacat gcggttgtag ttagtgtcat
    35041 tcagattgcg ctgtatttcg tcgttatcat ctacgctgcc ggtattaccc gcaaacggac
    35101 gattagagat caccgcatcg gcccacggtt tagccgtgtt aaaaccttcg ttgatggcgc
    35161 tatcacggat caccacctga ccgttggtat tggcatcaac atccagcgag cggcccagtt
    35221 gcgccacacc atcaccgaaa gcattgaaac ggctgtttac ggcgaggaaa ccgtagtaaa
    35281 tgttggacag cgtagccggt gcaaacacat acgcttcttg ctgagtacgt gagttcacca
    35341 cgcggaattc ggtgttatcg aacaccactg cgccgcgacc agaaacgata tccacatccc
    35401 cttcaatgta gctgttggtc accagcgtac gcggctgacg attcgtttcc agacggttct
    35461 gcacaccgct gttggtgaca aagaaggtgt tctgacgacc gagaatgtta acgttgttaa
    35521 tctgtacctg gtcaccatca gtacgcagtg ccaccgccgg atggttacct gcatctacgc
    35581 tatcgcccag cgtgttttcg atggtcagat tttgcagttg caggccattg ttttgtgacc
    35641 agaagaccgc agagcagaga acaccgatac tgtcgctgcg tttgctctgg cagctatcgt
    35701 acatatacca cgctggttta cctggcatat atttgccgcg cgggttgacg tcgtgacgcc
    35761 agtcggcagg gctcatgcca ccatcaaggg aaagcccaat cttcacatca atcggttttt
    35821 cacctgtacc gtacagagta attccacccg gagcggcagg gacatatacc gttccctgat
    35881 actcaccagg catcacggca atatactggc gcttgttggt acgcttgata attgccgcat
    35941 ctaccgccgc ctgaatcgtg gtatgcgtta caccttgagt gcccgccggg ccgacaacaa
    36001 agtcaggttg cgcaggcagg gtaatcgggg aaggattcca cgctgctgca cctggtgtca
    36061 gggatgcaaa atagtgttga gcatcgaaat tctgcgcttc ttttgccgac agaatcgggc
    36121 gagaagaggt accaggcgcg gtttgatcag aaggacgttg atcgggcggg gttgagctac
    36181 aggcggtcag cgtcacgcca aaagccaatg ccagcgccag acgggaaact gaaaatgtgt
    36241 tcacaggttg ctccgggcta tgaaatagaa aaatgaatcc gttgaagcct gcttttttat
    36301 actaagttgg cattataaaa aagcattgct tatcaatttg ttgcaacgaa caggtcacta
    36361 tcagtcaaaa taaaatcatt atttgatttc aattttgtcc cactccctgc ctctgtcatc
    36421 acgatactgt gatgccatgg tgtccgactt atgcccgaga agatgttgag caaacttatc
    36481 gcttatctgc ttctcataga gtcttgcaga caaactgcgc aactcgtgaa aggtcggcgg
    36541 atccccttcg aaggaaagac ctgatgcttt tcgtgcgcgc ataaaatacc ttgatactgt
    36601 gccggatgaa agcggttcac gatgagtaga tgcaattatg gtttctccgc caagaatctc
    36661 tttgcattta tcaagtgttt ccttcattga tatcccgaga gcatcaacat gcaatgttgt
    36721 agggatggca atttttacgc ctgttttgct ttgctcgaca taaagatatc catctacgat
    36781 atcagaccac ttcatttcgc ataaatcacc aactcgctgc ccggtaacaa cagccagctc
    36841 cattgcaagt cttagccaac atggtgatga ttctgctgct tgataaattt tcaggtattc
    36901 gtcagccgta agtcttgatc tccttacctc tgattttgct gcgcgagtgg cagcgaccgg
    36961 gtttgttgtt atatggcctt cagctattgc ctctcggaat gcatcgctca gtgttgatct
    37021 gattaacttg gctgacgccg ccttgccctc gtctatgtat ccactgagca ttgccgcaat
    37081 ttcttttgtg gtgatgtctt caagtggagc atcaggcagc cccctcctt
//
LOCUS       DAZGOB010000039        35773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGOB010000039 DAZGOB010000000
VERSION     DAZGOB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.039"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(18..93)
                     /locus_tag="ACHLIF_002042"
     tRNA            complement(18..93)
                     /locus_tag="ACHLIF_002042"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="ACHLIF_002043"
     tRNA            complement(152..228)
                     /locus_tag="ACHLIF_002043"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /gene="yifK"
                     /locus_tag="ACHLIF_002044"
     CDS             complement(331..1716)
                     /gene="yifK"
                     /locus_tag="ACHLIF_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9606058.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHLIF_002045"
     CDS             complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHLIF_002045"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO9606059.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHLIF_002046"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHLIF_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO9606060.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHLIF_002047"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHLIF_002047"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO9606061.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHLIF_002048"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHLIF_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO9606062.1"
                     /translation="MSLAKASLWTVASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPATIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHLIF_002049"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHLIF_002049"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO9606063.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHLIF_002050"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHLIF_002050"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO9606064.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHLIF_002051"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHLIF_002051"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO9606065.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHLIF_002052"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHLIF_002052"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO9606066.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHLIF_002053"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHLIF_002053"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO9606067.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHLIF_002054"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHLIF_002054"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO9606068.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHLIF_002055"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHLIF_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO9606069.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNEELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHLIF_002056"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHLIF_002056"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO9606070.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHLIF_002057"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHLIF_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO9606071.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHLIF_002058"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHLIF_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO9606072.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHLIF_002059"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHLIF_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO9606073.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHLIF_002060"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHLIF_002060"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO9606074.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="ACHLIF_002061"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="ACHLIF_002061"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO9606075.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHLIF_002062"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHLIF_002062"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO9606076.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22141..22590
                     /locus_tag="ACHLIF_002063"
     CDS             22141..22590
                     /locus_tag="ACHLIF_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO9606077.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            22789..23070
                     /gene="ppiC"
                     /locus_tag="ACHLIF_002064"
     CDS             22789..23070
                     /gene="ppiC"
                     /locus_tag="ACHLIF_002064"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO9606078.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23157..24632)
                     /gene="ilvC"
                     /locus_tag="ACHLIF_002065"
     CDS             complement(23157..24632)
                     /gene="ilvC"
                     /locus_tag="ACHLIF_002065"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO9606079.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24782..25675
                     /gene="ilvY"
                     /locus_tag="ACHLIF_002066"
     CDS             24782..25675
                     /gene="ilvY"
                     /locus_tag="ACHLIF_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO9606080.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(25727..27271)
                     /gene="ilvA"
                     /locus_tag="ACHLIF_002067"
     CDS             complement(25727..27271)
                     /gene="ilvA"
                     /locus_tag="ACHLIF_002067"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO9606081.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27274..29124)
                     /gene="ilvD"
                     /locus_tag="ACHLIF_002068"
     CDS             complement(27274..29124)
                     /gene="ilvD"
                     /locus_tag="ACHLIF_002068"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO9606082.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29189..30118)
                     /gene="ilvE"
                     /locus_tag="ACHLIF_002069"
     CDS             complement(29189..30118)
                     /gene="ilvE"
                     /locus_tag="ACHLIF_002069"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO9606083.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30138..30401)
                     /gene="ilvM"
                     /locus_tag="ACHLIF_002070"
     CDS             complement(30138..30401)
                     /gene="ilvM"
                     /locus_tag="ACHLIF_002070"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO9606084.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30398..32044)
                     /gene="ilvG"
                     /locus_tag="ACHLIF_002071"
     CDS             complement(30398..32044)
                     /gene="ilvG"
                     /locus_tag="ACHLIF_002071"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO9606085.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32047..32097)
                     /gene="ilvX"
                     /locus_tag="ACHLIF_002072"
     CDS             complement(32047..32097)
                     /gene="ilvX"
                     /locus_tag="ACHLIF_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO9606086.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32184..32282)
                     /gene="ilvL"
                     /locus_tag="ACHLIF_002073"
     CDS             complement(32184..32282)
                     /gene="ilvL"
                     /locus_tag="ACHLIF_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO9606087.1"
                     /translation="MTALLRVISLVMISVVVIIIPPCGAALGRGKA"
     gene            32635..34155
                     /gene="yifB"
                     /locus_tag="ACHLIF_002074"
     CDS             32635..34155
                     /gene="yifB"
                     /locus_tag="ACHLIF_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO9606088.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(34180..34518)
                     /gene="maoP"
                     /locus_tag="ACHLIF_002075"
     CDS             complement(34180..34518)
                     /gene="maoP"
                     /locus_tag="ACHLIF_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO9606089.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34637..35476
                     /gene="hdfR"
                     /locus_tag="ACHLIF_002076"
     CDS             34637..35476
                     /gene="hdfR"
                     /locus_tag="ACHLIF_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO9606090.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(35572..35647)
                     /locus_tag="ACHLIF_002077"
     tRNA            complement(35572..35647)
                     /locus_tag="ACHLIF_002077"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35612..35614),aa:Trp,seq:cca)
     gene            complement(35656..35732)
                     /locus_tag="ACHLIF_002078"
     tRNA            complement(35656..35732)
                     /locus_tag="ACHLIF_002078"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35696..35698),aa:Asp,seq:gtc)
BASE COUNT         8734 a         9752 c         9033 g         8254 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgcg
      301 cgtttggtta cggtttgtgc gttttgctga ttattcctcc agtttatgcg ctttcccgtg
      361 gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccaac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 tggtacgccg tgacgggaaa ctttcgccat cgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagataa ccaaagaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga cgaatagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcaaggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg gagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccactggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcagcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattagctc cctgacctca gcattatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcgtgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc atggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagg
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagac caaccacgat
     2941 cgccccgagc gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag cacttcccag gtaaagtagt tggctgagtt
     3061 cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggcgagaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccca caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctat cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggagaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgatgccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgccctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttcccgg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaca gcgtaccaat gccctgctgg
     4261 cgagcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgagg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcagccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgatttccgc cagaatataa aaccgcaatg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcagc cagacggtaa
     5401 aactcgccgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg atagtggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc tccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacacag
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccaccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgca
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtat agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttctac cacaaacaga
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcagcgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaaa acgatttttg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctac
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 ataaccgcga ataccgccgg acgccgcacg taaaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgtctg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacta cagcaccact atcactgaca ttgttcacgg gtagtgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcacccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacacgt ctggcgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa ggtactggct tcaatcaggc tgtcgtgagt gccagtgtcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttccagcga gatagcgcgg aaattgtcgt caaactccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaat accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cgttgaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcgtattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccaac atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtgccggaac cgcccgccag gataatacct ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgcac cgttttacgc attccacttt
     9121 caaaggtttc ctgtggcagc caaccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga tagtgcgcca
     9241 cgccgtgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctactggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagtgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggtcg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg tgggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagtt tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctttcacgcc tgtgaccggt caccagaatc atctttttat cggggtcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctgatcacg cacccataac
    11941 agtgcatcaa tgactgtatt accagtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg taaaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtt
    12181 gttgtgtcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc gccgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gagttggacc aacattcagg gtgtttaaca tggcccgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatgctgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcctca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg ggtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgctt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttggtcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgcgctcca ctcttgtcga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccact ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttag tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accgaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcggttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcacccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccacgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccatgccga
    17281 cgcagaaccg ctggcggaag ctaccggcct gaagctgggt ctggcttacg gtggtgatgg
    17341 ctacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcactacggg
    17401 gcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtggtggt
    17461 actggacgaa gccgatcgca tgtacgatct gggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa taccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccacg
    18181 tacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcatactg tagtaagtga ttcaggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaataa
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgccctgtct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgtcttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cacgtctcat ttatcagggc gttgctcaca caactggcgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacgctg cgtaatattc
    19321 agcgccgctt tatgatcgat attgatcagg cgcagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaag gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt taaacaagcg ccgcaacacg
    19501 ctgcttatct ggtgcgtaat ctggatcttc ccggttttac ccccgcacag aaaaaattgc
    19561 tggcgacgct actgctcaac cagactaatc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc tccgcgcgtc gcagaacaac tctgccgttt actccgcctg gccatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aagccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ttccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 gctgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagctcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatgctgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtgcagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcggctttc gggctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtaaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctctcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacactcgc gactgatggt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttaa gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttctc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctgac tcagcagcac caggttttgt ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcgtcat cgcccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatac tcatccacca gcagatagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagcagca acgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagaacatt gcacgctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac gatcccgctc gccctttgcc tctgccgccg cctgcgccgg
    21481 agttttgaga tcgttcttcc agttggagat ggtcgaaatc agctgttgca ggagaacttt
    21541 gtcatcttca atcagcccct cggtcaactc tttaagcaaa gcaagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatcccaag cgccgcatac tcgcgtttga tgatatccaa
    21661 ccccagcgtg tgaaaagtgg agatcatcaa cccgcgcgcc tctttgcgac ccagcgtctg
    21721 cccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga tcacacgagt
    21841 tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggtgt attgctcaat cttcgaacgg ggggaggatt
    21961 gtagcagaat cgcatttgaa tgtgaccgcg tgtaagccgc taaccggttg gctgcggatg
    22021 tttaaatcag agagagaaga ccgaaatcgg agttcatcgt gaatccatta gacttagaaa
    22081 atatcgggtc gtaacgttgt gttattactt tttcagagtt tactaaggag aggtttactc
    22141 gtggcgatta atttcagccc taaggttggt gaaatacagg aatgcaactt tggtaactat
    22201 cctgttatcc agaatggaca gttcagcacc acgtattacg atggccgcat tcctcctgaa
    22261 atgataaaaa acaggttagt tgtcgtgctc aacggaaaaa ttaatggcaa cgcatgtatc
    22321 gtcgttccat tatcaaccac tcgcgatcat gacaagttaa atcgtggaat gcatgttgaa
    22381 atagccagta atgttatcaa tggcctgcaa ttctttgatc agcagattcg ttgggccaaa
    22441 gccgacctgg tacaacaagt cagtcgaaac cgattaaata gagcaaggac ttacagaggg
    22501 tatcttaatc aatgtttgcc acatgagttg gttgcagaca tacagagggc ggtcatcaag
    22561 tcaatcaatg ccatatcgct cataaattga caataaaata accatccccg ataatgactt
    22621 tgtagcccat agaggcccta agttttctcc ccttgaggga cctgcgaagc ccggattttc
    22681 cgggcttttg ccatatatgt accagcccct ccctgactga caattacgtt agcatctgcg
    22741 gatcatgcta aaatcgccgc ctgacaatta ttcactcaag gcacgatcat ggcaaaaaca
    22801 gcagcagcac tgcatatcct tgtaaaagaa gagaaactgg ctctggatct tctcgagcag
    22861 attaagaacg gtgccgattt cggcaagctg gcgaagaaac actctatttg cccgtcaggc
    22921 aaacgcggcg gtgatttagg tgaattccgc cagggtcaga tggttccggc gttcgataaa
    22981 gtggttttct cttgtccggt actggagccg accggcccgc tgcacaccca gttcggatat
    23041 cacatcatta aggtgctgta ccgcaactaa tagcaaggcc ttctccagga gaaggccttg
    23101 agtgttttct ccctctccct gtgggagagg gtcggggtga gggcatcagc gcgcacttaa
    23161 cccgcaacag caatacgttt catatctgtc atatagccgc gcagtttctt acctacctgc
    23221 tcaatcgcat ggctgcgaat cgcttcgttc acatcacgca gttgcgcgtt atctaccgcg
    23281 ccttccggaa tagctttacc caagtcgccc ggttgcagct ctgccataaa cggtttcagc
    23341 aacggcacac aagcgtaaga gaacagatag ttaccgtact cagcggtatc agagataacc
    23401 acgttcattt cgtacagacg cttacgggcg atggtgttgg caatcagcgg cagctcgtgc
    23461 agtgattcat aatatgcaga ctcttcaatg atgccggaat cgaccatggt ttcgaacgcc
    23521 agttcaacgc ccgcttttac catcgcgatc atcagtacgc ctttatcgaa gtactcctgc
    23581 tcgccgattt tgccttcata ctgcggcgcg gtttcaaacg cggttttgcc ggtctcttca
    23641 cgccaggtca gcagtttctt atcatcgttg gcccagtccg ccatcatacc ggaagagaat
    23701 tcgccggaga tgatgtcgtc catatgtttc tggaacagcg gtgccatgat ctctttcagc
    23761 tgttctgaca gcgcataagc acgcagtttc gccgggttag agagacggtc catcatcagg
    23821 gtgatgccgc cctgcttcag cgcttcggtg atggtttccc aaccgaactg aattagtttt
    23881 tctgcgtatg ccggatcggt gccctcttcc accagcttgt cgaagcacag cagcgagcca
    23941 gcctgcaaca taccgcacag gatggtttgc tcacccatca ggtcagattt cacttccgca
    24001 acgaaggacg attccagcac gcccgcacgg tgaccaccgg ttgctgccgc ccatgcttta
    24061 gcgatcgcca taccttcgcc tttcggatcg ttttccgggt gaacggcaat cagcgtcggt
    24121 acgccgaatc cacgtttgta ctcttcacgc acttcggtgc cagggcattt cggcgcaacc
    24181 atgactacgg tgatatcttt acggatctgc tcgcccactt cgacgatgtt gaaaccgtgc
    24241 gagtaaccca gcgccgcgcc gtctttcatc agtggctgta cggtgcgcac tacatcagag
    24301 tgctgcttgt ccggcgtcag gttaaccacc agatccgcct gcgggatcag ttcttcgtaa
    24361 gtaccaactt taaaaccgtt ttcggtcgct ttacgccagg acgcgcgctt ctcggcaatc
    24421 gcttctttac gcagagcgta ggagatatcc agaccagaat cacgcatgtt caggccctgg
    24481 ttcagaccct gtgcgccaca gccgacgatg actacttttt taccctgaag gtagctcgcg
    24541 ccatcggcga attcatcgcg ccccataaag cgacatttgc ccagctgtgc cagctgctga
    24601 cgcagattca gtgtattgaa gtagttagcc atggtgattc ctcgtgatgt tgtgcttctt
    24661 attgttcggt tcgcgtttgc gagattgaat tcactatatg acaggaaatt tattgcggaa
    24721 attgatatat tcacaacgtc acattgcaat ttttgcaacg tcaacatcga gggctgcgcc
    24781 attggattta cgcgatctga aaaccttcct gcatctggcg gaaagccgcc attttggccg
    24841 cagcgcgcgg gcgatgcacg ttagcccatc cacgctctca cggcagattc agcgcctgga
    24901 agaagatctt ggtcagccgc tatttgtgcg cgataaccgc acggtaacgc tgacggaagc
    24961 gggcgaagag ctgcgcgttt tcgcccagca aacgctgttg cagtatcagc aattgcgcca
    25021 caccatcgat cagcaagggc cgtcgctctc tggcgaatta catatcttct gctcggtgac
    25081 cgctgcctac agccatctgc cgccgattct ggatcgcttc cgcgcggaac acccgtcggt
    25141 ggagatcaaa ctcaccactg gtgatgcggc agatgcaatg gaaaaagtgg tcacgggaga
    25201 agcggatctg gcgattgccg ggaaaccgga gacgctaccc ggcgctgtgg cgttttcgat
    25261 gctggagaat ctggcagtag tgctgattgc gcccgcgctg ccctgcccgg tgcgtaacca
    25321 ggtttcggca gagaagccgg actggtctac agtgccgttt atcatggccg atcagggacc
    25381 ggtgcgtcgc cgcattgaac tgtggtttcg acgcaataaa atcagcaacc cgatgattta
    25441 cgccacggtt ggcgggcatg aagcgatggt atcgatggtg gcgctcggct gtggtgtggc
    25501 gctgttgccg gaagtagtgc tggaaaacag ccccgaaccg gtgcgtaacc gcgtgatgat
    25561 tttagagcgc agcgatgaaa aaacgccgtt tgaactgggc gtgtgtgcac aaaaaaagcg
    25621 gctgcatgag ccgctgattg aggcgttctg gaggattttg ccgaaccaca aatgacgttg
    25681 tcgcgcgggt aggcctgata agcgaagcgc tatcaggcat ttttccctaa cccgccaaaa
    25741 agaacctgaa cgccgggtta ttggtttcgt cgtggcaatc gtagcccagc tcattcagcc
    25801 gggtttcgaa atccggttca tggtcgccaa gttcgaacgc cgccagtacg cgcccgtagt
    25861 cggtgccatg gctgcgatag tggaacaaag aaatgttcca gtgcgtaccc agcgtgttga
    25921 ggaagcgcag cagcgcgccc ggtgattccg ggaattcgaa gctgtagagg cgttcctgca
    25981 acggatgtga cggacgcccg ccgaccatat agcgcacatg caacttcgcc atttcgtcgt
    26041 cggagagatc caccacgctg tagccgccgt cgttgagcat ctgcaaaatt tctttgcgct
    26101 cttcgaggcc gcggctcagg cgcacgccga caaagatgca ggcgtttttg gcatcggcaa
    26161 aacggtagtt gaactcggtg accgatcgcc cgccaagcag ttggcagaat ttgaggaagc
    26221 tgcctttttc ttccggaatg gtcaccgcca acaacgcttc acgctgttcg cccagttcgc
    26281 agcgttccga gacgtagcgc aggccgtgga agttcacgtt ggcaccggaa agaatatgcg
    26341 ccagccgttc gccgcgaatg ttgtgctggg cgatatattt tttcattccc gccagcgcca
    26401 gcgcgccaga gggttccgcc accgcgcgca catcttcgaa taaatccttc atcgccgcac
    26461 agatcgcatc actatcgacg gtgatgatgt cgtcgagata ctcctggcat aaacggaagg
    26521 tttcgtcacc gatgcgtttt accgctacgc cttcagcaaa tagccctacg cgcggcagat
    26581 caaccggatg acccgcatcc agcgctgctt tcaggcaggc ggagtcttcc gcttctacgg
    26641 cgatcacttt gatttgtggc atcagttgtt tgatcagcac cgcaacgccc gccgccagac
    26701 cgccgccgcc gactggcaca aatacgcggt cgagatgggc gtcctgctgg agcagttcca
    26761 gcgccagcgt gccttgcccg gcaatcacca tcggatggtc gaacggcggc acccaggtga
    26821 acccctgctg ctgtgacagt tcgatcgctt tagctttcgc ttcgtcaaag ttcgcgccat
    26881 gaagcagcac ttcgccgccg aatccacgta ccgcatcgac tttgatgtcg gcggtggcgg
    26941 ttggcataac gatcagggcc ttcacgccta accgcgcaga agaaaacgcg acgccctgcg
    27001 cgtggttacc cgcagaagca gtgatcacgc cgtgcgcttt ttgttcttcc gtcaggcccg
    27061 ccatcatggc gtatgcgccg cgcagcttaa agctgtgcac tggctggcga tcttcgcgct
    27121 tcaccagaat gacgttatca agacgcgacg acagtttttc cattttttgt agcggcgtaa
    27181 cctgcgccgc ctcgtaaacc ggcgcgcgca gcactgctct taaatattcg gcaccttccg
    27241 gagcaccgga caggggttgc gagtcagcca ttattaaccc cccagtttcg atttatcgcg
    27301 caccgcacct ttgtcggcgc tggttgccag gctggcgtag gcgcgcaggg caaaggaaac
    27361 ctgacgttca cggtttttcg gcgtccaggc tttgtcacct cgagcgtcct gcgcttcacg
    27421 acgcgccgcc agttcggcat cgcttacctg taactgaatg ccacggttcg ggatgtctat
    27481 agcgatcagg tcaccatctt caatcaggcc aatgctgccg ccgcttgccg cttccggtga
    27541 gacgtggccg atggaaagac cagaggtgcc gccagagaaa cgaccgtcgg tgatcagcgc
    27601 acaggcttta ccgagaccca ttgatttcag gaagctggtt gggtagagca tttcctgcat
    27661 gccagggccg cctttcgggc cttcatagcg gatcaccacc acatcgcccg cgacaacttt
    27721 gccaccgagg atcgcttcta ccgcgtcgtc ctggctttcg tacactttcg ccgggccggt
    27781 gaatttgagg atgctgtcat cgacgcccgc ggttttaacg atgcagccgt tttctgcgaa
    27841 attaccgtag agcaccgcca ggccgccgtc tttgctgtag gcgtgttcca gcgagcggat
    27901 acagccattg gcgcgatcgt catcgagaga atcccaacgg caatcctgcg agaatgcctg
    27961 tgtggtacga atgcctgcag gacctgcgcg gaacatattt tttaccgcgt catcctgggt
    28021 cagcataacg tcgtattgtt ccagcgtttg cggcaacgtc aggccaagta cgtttttcac
    28081 atcacggttc agtaaccccg cgcgatccag ttcgccgaga ataccgataa caccaccagc
    28141 acggtgaaca tcttccatat ggtatttctg ggtgctcggc gcaactttac acagctgtgg
    28201 aaccttgcgg gaaagcttat cgatatcact catggtgaag tcgatttccg cttcctgcgc
    28261 cgccgccagc aggtgaagta cggtgttagt cgatccaccc atcgcgatat ccagcgtcat
    28321 ggcgttttca aacgccgctt tgctagcaat attgcgcggc agtgcacttt cgtcgttttg
    28381 ctcgtagtaa cgtttggtca attcaacaat gcgtttaccg gcattgagga acaactgctt
    28441 acggtcggcg tgggttgcca gcagcgagcc gttgcccggc tgcgacagac ccagcgcttc
    28501 ggtcaggcag ttcattgagt tagcggtaaa catcccggag caggagccgc aggtcggaca
    28561 cgcggaacgt tcaacctgat cgctctggga gtcagaaact ttcgggtctg cgccctggat
    28621 catcgcatca accagatcga gtttgattat ctgatcggac agtttggttt tcccggcctc
    28681 catcgggccg ccggaaacaa agatcaccgg aatattcagg cgcagggaag ccatcagcat
    28741 ccccggggtg attttgtcgc agttagagat gcagaccatg gcgtcggcgc agtgggcgtt
    28801 gaccatatac tcaacggaat cagcgatcag ttcgcgagat ggcagtgaat aaagcatccc
    28861 cccgtggccc atggcaatcc catcatccac cgcaatggtg ttgaactctt tggcaacgcc
    28921 gccagccgct tcaatttgtt cggcgaccag tttaccgaga tcgcgcagat ggacgtgacc
    28981 cggtacaaat tgggtgaacg agttcacaac cgcgataatc ggcttgccaa aatcggcgtc
    29041 ggtcattccg gtggcgcgcc acagcgcacg agcacccgcc atattacgac catgagtggt
    29101 ggtggcggaa cggtacttag gcatacttta tttactccca gtgtctgtct cgtaaatggg
    29161 acggtgcgtg ccgtcccatt ttttgtattt attgattaac ttgatctaac cagccccatt
    29221 tatcttcggt ttcgccagtg aagaggccga agaaggcttg ctgaatgcgt ttggtaaccg
    29281 ggccacaacg gccttcgcca acctgaatac catctacact gcgcactggc gtgatttctg
    29341 ccgcagtacc ggacataaac acttcatccg ccaggtacag ggattcgcgc gacagaacct
    29401 gctcacgcac ttcaattccc agctctttcg ccagtttgat gatggcatca cgggtaatac
    29461 ccggcagcgc ggaggaggtg aacggtgggg tgaacagcac accatctttc acttcaaaca
    29521 ggttttcgcc tgcgccttca gagatataac cgttcacatc cagcgcgata ccttcctgat
    29581 aaccgtggcg gcgcgcttcg ctacccacca gcagggaaga gagatagtta ccaccggctt
    29641 ttgccgcggt tgggatggtg tttggtgctg cgcggttcca ggaggaaacc atcgcatcga
    29701 tcccctgctc cagcgcttct gcgcccagat acgctcccca cgggaaagcg gcgataatca
    29761 catcggttga gtatcccgct ggcgggttta cacccatgcc aacatcacca acgaagatca
    29821 gcggacggat ataggcgctg gtgagattgt ttttgcggat cacgtcacga caagcttcca
    29881 tcagctcatc aatgctctgc gaaaccggga agcgatagat tttggcggag tcatgcagac
    29941 gctgcatatg ctcacgatgg cggaatacaa ccggtccttt gtgcgagtcg tagcaacgga
    30001 tgccttcaaa aaccgaagtg ccatagtgca gcgcgtgcga catcacatgc accttcgcgt
    30061 cttcccagcg aaccatctcc ccattgaacc aaatgtaatc agctttcttc gtggtcattt
    30121 ttatattcct tttgcgctca ggcgcggatt tgttgtgatg tggttgtgct ctggcagatg
    30181 gcaacgtgtg cgacgtccac cagtttattt aactgactaa acagtaagtc gaccgaccgt
    30241 gggctggcaa cggtcaattc gatatttata ttttgtgcat cgctggcggc agccatattc
    30301 attgagcaga cgtggaaacc acgatgacgc accacgcgta aaacacgttc taaggtttcc
    30361 ggattgaagc gagccgatac attgacctga tgttgcatca tgataatttc tccaacattt
    30421 ctgaattact ggcgccaggc ggcaccagcg gccagacgtt ctcaagttcg tcgattgaga
    30481 catgaagcag gtatggccca tcactgttca gcatggtgtc gagtgccgct tcaacctggt
    30541 ctttacgggt gatgtgttgg ccagggatgc cgaaggcgct ggctaacatg aggaaatcgg
    30601 ggttatcagt aagggtggtt tcgctgtatc gttcctgaaa aaacagttgc tgccattgtc
    30661 gaaccatccc taaccgttgg ttatcgagta agacgatttt caacggtaac tgcttgcgtt
    30721 ttacggtgcc cagctcttgc acattcatca tgaaagagcc gtcaccggag atacagacga
    30781 cagtatcgtt cggtcgtgcg acttgtgcgc caaccgccgc tggtaaaccg aaacccatgg
    30841 tgcctaagcc gctggaggta atgaaatttt ccgggcgagt gtgtgcgatg tgctgcgcgg
    30901 cccacatctg gtgctgcccc acatctgtgg tcacgacgca atccgcaggt ttacgatccg
    30961 acagttgttt taacaacaac ggcgcgtaga tagcgtcacc gggatggtcg taacgccagg
    31021 tatgttcatc acgcagctgc gcgcagtatt gctgccagtc attgatattt aacggctgct
    31081 gtaatgctgg taacagagca tttaaatcac cttgtaatgc cacatgtgcc tgacgcagct
    31141 tgttcatttc tgccgggtcg atatccatat ggataacact ggcgtgtggt gcgaaggtgt
    31201 tcagtttgcc ggtcacccgg tcatcaaaac gtgcacccac ggcgatcagc aagtcgcact
    31261 cctgcaccgc gaagttcgcc gctttggtgc catgcattcc cagcatgccc aggtagtacg
    31321 gataatctgc ttcaactgcg cccagccctt tcagcgtgca ggtggcaggc atttttgtgg
    31381 tagcgagaaa ttctcgtaaa gcaggaactg cctgcgccat acccacgcca ccaccaacgt
    31441 acagcatcgg tttttgcgct tttgccagca tctggcgcgc ttgctcaact tcggcatgtg
    31501 ggaaagtcac ttcgttttca acggtggtga accacggttc caggtcaccg ctggctagct
    31561 ggatatcttt tgggatatcg accagaaccg gaccaggacg acctgagctg gcaacgtcga
    31621 atgcttcagc cataatgcgc ggcaactctt ccagcgactg caccagaaag ctgtgcttgg
    31681 tacaggctaa cgacaatccc aggacatcca cttcctgaaa tgcgtccgtg ccgataaacg
    31741 gtgcggacac ttgaccggtg atggcaacaa cagggataga atctaacagt gcgtccgcaa
    31801 gcccggttat caggttggtt gcgcccggac cagacgtggc gatacatacg ccagttttgc
    31861 cggtagcacg ggcataaccg atagccgcca ttgccgcacc ctgctcatgt cggcacagca
    31921 agtgctccac gccgccgtca tacaatgcat cgtaaaccgg cataattgcg ccacccggat
    31981 aaccgaaaac ggtgttcaca ccctgtgccc gcaacgcatg taccacccac tgtgcgccat
    32041 tcatagttag ttccccgtcc tgaatcttga gaaacagaat tttgtgctgt tattcattgt
    32101 ctgctcctcg gttatatttt taaggtcaaa aaaaaccccc ggacctttcg gtgcgggggt
    32161 cttagttcgt taaggcttga tctctaagcc tttcctcgtc caagtgcagc cccgcacggt
    32221 gggataataa tcaccaccac gctaatcatg accaggctaa tcactcgtag aagggctgtc
    32281 attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa agagttacca tagattttgc
    32341 gaatagcaca agatattttt tggccgattt ttatgatgcg tttaataatt tcaatagaaa
    32401 ttgttgtttt ttaaaaagaa aatagtgagt gaaaaaaatt caatttcagt atgaatgttg
    32461 ttcccttcct cgtagttcat cctttgtccg ctgcattttt gcgagcatca ttccaccgcc
    32521 agaacaactc aacttcactt gcacaaataa acaggaatca gccactaaaa ttcacgaatt
    32581 agcgggctac agcagcgata tcttatgcac ataatagtga ctcaaggagg gcttatgtca
    32641 ctgtcaattg ttcatacccg cgcagccctg ggagtaaatg cgcccccaat cactgttgag
    32701 gtacatatca gtaaaggtct acccggctta acgatggtgg gcttaccaga aacaactgta
    32761 aaagaagctc gcgatcgcgt gcgcagcgcc attatcaata gcggatatga atatccggcg
    32821 aaaaaaatca ccatcaacct ggctccagcc gatctgccaa aagaaggggg acgatatgat
    32881 ttacctatcg ccattgcgtt gctggcggcc tcagaacagc ttacagccaa taagttagat
    32941 gaatatgaat tagtcggaga actggcgctt acaggcgctc tgcgtggcgt tcccggcgca
    33001 atctccagtg caactgaagc tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac
    33061 gaagatgaag tggggctaat taacggtgaa ggatgcctga tagccgatca tctgcaggct
    33121 gtctgtgcgt ttctggaagg taagcacgct ctcgaacgcc cgaaaccaac tgatgcagta
    33181 tcccgggcgc tacaacatga tctcagtgat gttgtcggtc aggagcaagg aaagcgagga
    33241 ctggaaatta ccgccgctgg cgggcacaac cttttactga ttgggccgcc gggaacaggt
    33301 aaaacaatgc tcgccagccg tattaatggc ctgttgccag atttaagcaa tgaagaggca
    33361 ctggagagcg ctgcgatatt aagtctggta aatgctgaat cagtacaaaa acaatggcgg
    33421 cagcgcccgt tccgctcacc gcatcacagt gcgtcgttaa ctgcgatggt gggcggtggt
    33481 gcaattccag ggccaggtga aatttcgttg gcgcataacg gcgtgctttt tcttgatgag
    33541 ctacctgaat ttgaacggcg tacactggat gccttgcgag agccgattga atccgggcag
    33601 atccatcttt cacgcacacg agccaaaata acctatccag cccgtttcca gcttgttgcg
    33661 gcgatgaatc ccagccctac cggacattat cagggaaacc ataaccgctg cacgccagaa
    33721 cagacattac gttatctcaa ccggctctcg gggccctttc tcgaccgctt cgatctctca
    33781 ctggagatcc cattaccacc ccccggcatt ttgagtaaaa cggtagtgcc gggagaaagc
    33841 agcgccaccg ttaaacaacg cgtaatggcc gccagagagc gccaatttaa gcggcagaat
    33901 aaactgaatg cctggctgga tagtccggaa atacgccaat tctgcaaact tgagagcgaa
    33961 gatgcgatgt ggttggaagg aacactgatc catctggggt tatcgattcg tgcctggcag
    34021 cggttattga aagttgcacg aaccattgct gatattgatc agtctgacat tatcacacgt
    34081 cagcatttgc aggaggcagt tagctatcgt gcgattgacc gtttgctcat ccatctgcaa
    34141 aaactactga cataaaaaaa gggcatttcg ccctttttat taatcatcag aatcggtgta
    34201 gtcttcagca ccttcaacct gcggtttacc gccggaaagg gtgtgaaaac gttttggacg
    34261 cttaatacgc gtcatatact tggaccacac gcgttctgct tctgtcactg gctcacgttc
    34321 gccacggcat actgctacga agagtttctc ttcctcggta accggctcgc gtttgccaag
    34381 atccaactca ttgaaggcat aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa
    34441 atcaccatga cgagagaatc cacgtggata atgtttattg tcgaaatatc gattagtcgt
    34501 cgtaaagctt tccgccatcc tgcacgctcc taattctttg accgagctag ttatggcgcg
    34561 gagtattagt tacgcttgac agagtgtaaa acaaaacatt taaatcataa cgacaaataa
    34621 ttttgcggag agcactgtgg atacggaatt gttaaaaact ttcctggaag ttagccgaac
    34681 gcgtcacttt ggtcgagcgg ctgaatcgct ctatctgacc cagtcagcag tgagctttcg
    34741 aatcagacaa ctggaaaatc aactgggtgt gaaccttttc acccgccaca gaaacaatat
    34801 ccgtttaacc gctgccggtg aaaaactact gccttatgca gaaacgctca tgagcacctg
    34861 gcaggccgcc cgcaaggagg tggcgcatac ctcacgacat aacgagtttt ctatcggtgc
    34921 cagcgcctca ttgtgggaat gtatgcttaa tcagtggctg ggacgtttgt atcaaaatca
    34981 ggatgcccat acaggcttac agttcgaagc gcgaattgcc caacggcagt ctctggtaaa
    35041 acagctgcat gaacgccagc ttgatcttct tattaccact gaagcgccca aaatggacga
    35101 atttagtagt cagttgctgg gatatttcac tttagcgctt tataccagtg ccccttcaaa
    35161 actaaaggga gatcttaatt atctgcgact tgagtggggg ccagattttc aacagcatga
    35221 ggcaggtttg atcggtgctg acgaagtgcc cattctgaca accagttctg ctgaactggc
    35281 acagcaacag attgcgatgc ttaatggttg cacctggcta cccgtcagct gggcgcgtaa
    35341 aaaaggcggc ctgcataccg ttgtcgatag cacaacactt tcacggccgc tttatgccat
    35401 atggctgcaa aatagcgata aaaatacgtt gattcgcgat cttttgaaaa ttaacgtgct
    35461 ggatgaagtg tattaatatg aatggctggc aaggatgccg gtagaaggat ttacttcgga
    35521 gagggttatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggcagggg
    35581 cggagagact cgaactccca acacccggtt ttggagaccg gtgctctacc aattgaacta
    35641 cgcccctaat tagggtggcg gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag
    35701 gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac caccggtttt
    35761 atgaccggct tac
//
LOCUS       DAZGOB010000040        35663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGOB010000040 DAZGOB010000000
VERSION     DAZGOB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.040"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..670)
                     /locus_tag="ACHLIF_002079"
     CDS             complement(<1..670)
                     /locus_tag="ACHLIF_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO9606091.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFH"
     gene            complement(873..1274)
                     /gene="nikR"
                     /locus_tag="ACHLIF_002080"
     CDS             complement(873..1274)
                     /gene="nikR"
                     /locus_tag="ACHLIF_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO9606092.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1280..2086)
                     /gene="nikE"
                     /locus_tag="ACHLIF_002081"
     CDS             complement(1280..2086)
                     /gene="nikE"
                     /locus_tag="ACHLIF_002081"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO9606093.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(2083..2847)
                     /gene="nikD"
                     /locus_tag="ACHLIF_002082"
     CDS             complement(2083..2847)
                     /gene="nikD"
                     /locus_tag="ACHLIF_002082"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO9606094.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2847..3680)
                     /gene="nikC"
                     /locus_tag="ACHLIF_002083"
     CDS             complement(2847..3680)
                     /gene="nikC"
                     /locus_tag="ACHLIF_002083"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO9606095.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3677..4621)
                     /gene="nikB"
                     /locus_tag="ACHLIF_002084"
     CDS             complement(3677..4621)
                     /gene="nikB"
                     /locus_tag="ACHLIF_002084"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO9606096.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4621..6195)
                     /gene="nikA"
                     /locus_tag="ACHLIF_002085"
     CDS             complement(4621..6195)
                     /gene="nikA"
                     /locus_tag="ACHLIF_002085"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9606097.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6306..6893)
                     /gene="acpT"
                     /locus_tag="ACHLIF_002086"
     CDS             complement(6306..6893)
                     /gene="acpT"
                     /locus_tag="ACHLIF_002086"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO9606098.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWH
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6948..7997)
                     /locus_tag="ACHLIF_002087"
     CDS             complement(6948..7997)
                     /locus_tag="ACHLIF_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO9606099.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            8129..9346
                     /locus_tag="ACHLIF_002088"
     CDS             8129..9346
                     /locus_tag="ACHLIF_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9606100.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9350..9907)
                     /gene="dcrB"
                     /locus_tag="ACHLIF_002089"
     CDS             complement(9350..9907)
                     /gene="dcrB"
                     /locus_tag="ACHLIF_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO9606101.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9980..10645)
                     /locus_tag="ACHLIF_002090"
     CDS             complement(9980..10645)
                     /locus_tag="ACHLIF_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO9606102.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10866..11111
                     /gene="tusA"
                     /locus_tag="ACHLIF_002091"
     CDS             10866..11111
                     /gene="tusA"
                     /locus_tag="ACHLIF_002091"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO9606103.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(11213..13411)
                     /gene="zntA"
                     /locus_tag="ACHLIF_002092"
     CDS             complement(11213..13411)
                     /gene="zntA"
                     /locus_tag="ACHLIF_002092"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO9606104.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13485..14111)
                     /locus_tag="ACHLIF_002093"
     CDS             complement(13485..14111)
                     /locus_tag="ACHLIF_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO9606105.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14252..14611
                     /locus_tag="ACHLIF_002094"
     CDS             14252..14611
                     /locus_tag="ACHLIF_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO9606106.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14614..14883)
                     /locus_tag="ACHLIF_002095"
     CDS             complement(14614..14883)
                     /locus_tag="ACHLIF_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO9606107.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14873..15469)
                     /gene="rsmD"
                     /locus_tag="ACHLIF_002096"
     CDS             complement(14873..15469)
                     /gene="rsmD"
                     /locus_tag="ACHLIF_002096"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO9606108.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15619..17115
                     /gene="ftsY"
                     /locus_tag="ACHLIF_002097"
     CDS             15619..17115
                     /gene="ftsY"
                     /locus_tag="ACHLIF_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO9606109.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            17118..17786
                     /gene="ftsE"
                     /locus_tag="ACHLIF_002098"
     CDS             17118..17786
                     /gene="ftsE"
                     /locus_tag="ACHLIF_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO9606110.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17779..18837
                     /gene="ftsX"
                     /locus_tag="ACHLIF_002099"
     CDS             17779..18837
                     /gene="ftsX"
                     /locus_tag="ACHLIF_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO9606111.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILMLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            19082..19936
                     /gene="rpoH"
                     /locus_tag="ACHLIF_002100"
     CDS             19082..19936
                     /gene="rpoH"
                     /locus_tag="ACHLIF_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO9606112.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            20207..21310
                     /gene="livJ"
                     /locus_tag="ACHLIF_002101"
     CDS             20207..21310
                     /gene="livJ"
                     /locus_tag="ACHLIF_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO9606113.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21509..21892)
                     /gene="panM"
                     /locus_tag="ACHLIF_002102"
     CDS             complement(21509..21892)
                     /gene="panM"
                     /locus_tag="ACHLIF_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO9606114.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            22316..23425
                     /gene="livK"
                     /locus_tag="ACHLIF_002103"
     CDS             22316..23425
                     /gene="livK"
                     /locus_tag="ACHLIF_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO9606115.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            23473..24399
                     /gene="livH"
                     /locus_tag="ACHLIF_002104"
     CDS             23473..24399
                     /gene="livH"
                     /locus_tag="ACHLIF_002104"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO9606116.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            24396..25673
                     /gene="livM"
                     /locus_tag="ACHLIF_002105"
     CDS             24396..25673
                     /gene="livM"
                     /locus_tag="ACHLIF_002105"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO9606117.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            25670..26437
                     /gene="livG"
                     /locus_tag="ACHLIF_002106"
     CDS             25670..26437
                     /gene="livG"
                     /locus_tag="ACHLIF_002106"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO9606118.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            26439..27152
                     /gene="livF"
                     /locus_tag="ACHLIF_002107"
     CDS             26439..27152
                     /gene="livF"
                     /locus_tag="ACHLIF_002107"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO9606119.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            27548..28864
                     /gene="ugpB"
                     /locus_tag="ACHLIF_002108"
     CDS             27548..28864
                     /gene="ugpB"
                     /locus_tag="ACHLIF_002108"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO9606120.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            28962..29849
                     /gene="ugpA"
                     /locus_tag="ACHLIF_002109"
     CDS             28962..29849
                     /gene="ugpA"
                     /locus_tag="ACHLIF_002109"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO9606121.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            29846..30691
                     /gene="ugpE"
                     /locus_tag="ACHLIF_002110"
     CDS             29846..30691
                     /gene="ugpE"
                     /locus_tag="ACHLIF_002110"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO9606122.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            30693..31763
                     /gene="ugpC"
                     /locus_tag="ACHLIF_002111"
     CDS             30693..31763
                     /gene="ugpC"
                     /locus_tag="ACHLIF_002111"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO9606123.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            31760..32503
                     /gene="ugpQ"
                     /locus_tag="ACHLIF_002112"
     CDS             31760..32503
                     /gene="ugpQ"
                     /locus_tag="ACHLIF_002112"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO9606124.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLWILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32490..32930)
                     /locus_tag="ACHLIF_002113"
     CDS             complement(32490..32930)
                     /locus_tag="ACHLIF_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO9606125.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            33050..34792
                     /gene="ggt"
                     /locus_tag="ACHLIF_002114"
     CDS             33050..34792
                     /gene="ggt"
                     /locus_tag="ACHLIF_002114"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO9606126.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(34830..35114)
                     /locus_tag="ACHLIF_002115"
     CDS             complement(34830..35114)
                     /locus_tag="ACHLIF_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO9606127.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(35314..35469)
                     /gene="yrhD"
                     /locus_tag="ACHLIF_002116"
     CDS             complement(35314..35469)
                     /gene="yrhD"
                     /locus_tag="ACHLIF_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HFO9606128.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT         8300 a         9260 c         9938 g         8165 t
ORIGIN      
        1 ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc cggtacggcg
       61 gcagcggcgc aggcagcacc tcatccgctt ccggcacccg ctcacaccag ccgagtaccc
      121 accacggccc ctgcggactg tttgtcgcca gataacgatg cggactcagg cgaagctctt
      181 ccggcagcgc ctgccagagt gcggccagcc ggtgaccttc atccagtttc gccacaccgc
      241 cgcgcaccag ccagagtgat tcgctgcggc tgtaaccgtc ctcaccggga aacaggtgct
      301 caaaatacag gcttctgccg ccgttatcac tgagtatcag tgtgttatcg cgcagctgta
      361 agcggatatc cgcaggcatt ttccagccgg ggccgaggct ccccaccggc gcgggcgttt
      421 ttgtccggta actgctgtag gtgcgggaga gaatgaacgg cagcgggccg ggcagggcga
      481 tgtcggtttc accgggaagg acctttgcac ccagcagcgg attgaccgga tggccggacg
      541 tcaccccgcc ggggcacacc gaacaggcca cgccggtggg ggcaccaata cgtaccccgg
      601 ctgaaccctg aacaatgcta ccgccatact gcgtcatgtc accctgacgc gccgccggtt
      661 ttccgctcat cgtaaaatcc ttttctatta ttttaatgac agtatcttca tacgaaaaag
      721 cgaagaaaaa cttgtggtca caatgaattg aagattttta ggaatatatt tagaacgtta
      781 caaaagagaa ggttcggata gggtatttag agaggaatca ggtgtgctag tggccctttg
      841 ttgatcgttc accaaaggac ccaataatag actcaatctt ccttcggcaa gcactgcaaa
      901 tgcccgtgcc gcacgccgcg ctgggcgata acgtcatcgg caaaatgctg cacgtcgccc
      961 atatcacctt tcagcacggc gatttccaga cagtcgtcgt gattgatatg cacatgcagc
     1021 gtggcgacgg agagatcgtg gtgatggtgc tgggtggaga caatgcggct ggctaagtcg
     1081 cgtttttcgt gttcatacac ataggagagc accgcgaaac cttgcgtgcc gtgctgctga
     1141 gtggcctcct gggccagagc gctacgcaga atatcgcgga tagcttcgga acgattgtta
     1201 taaccacgac gctggctcag gctgtccagc gtctccagta aatcgtcatc aagcgtgatg
     1261 gtgactcgtt gcatttgcgt taaacctttt ctgtggtgcg acggcgcacg gggaatgcgg
     1321 gtaataccgc gttttgtagc acacgtccgg cgtcagagga aaaggttaat ttatctccca
     1381 ccacctgggt ttcgacgatt tgtccgttgt ccatcaccat cacccgctga cagaagcgtt
     1441 ccaccaggcg taagtcgtgg gtgatgaaca ggcaggcggt gccaaactgt tgttgtagct
     1501 ttttcagcag gcgaatgacg cccgcctgta acacgagatc aaggttagaa acggcttcat
     1561 ccagaatcag tagtttcggt tcgaccgcca gcgcgcgagc caggcagacg cgctggagct
     1621 ggccgccgct taactgcggt gggcgtttgt cgagaacgct gtcatcgaga tcgaccgcct
     1681 tcagcatttc gctggcgcgc gccagttgtt cggatttttt cagtgatagc aggtggcgca
     1741 tcggttcacg caggatctcg cgcacggttt tgcgcggatt cacggcgctg atggagtcct
     1801 gaaataccat ctgaatatcg cggcggaacg ctttacgctg ggcgcgattg agtttcgcca
     1861 gcggttcgcc acgccagcta atattcccct gcgcgggcga ttctaaaccc accagcaacc
     1921 gcgcgagggt acttttcccg cagccgctgc gccccagcag ggcgacggtt tcgccgcttt
     1981 tcagggtcag ggaaacgtta ttcagcaccg cctgatgttg atgttttccg ttaaatccac
     2041 cgtgcgcata gtgatgggaa aggccggaga tgttaagtaa agtcatgatg ccagctccat
     2101 accgtagagg gcgagatgag cggaaaccag gctgcgcgtc actgtatgtt tgggggcgtt
     2161 aaacagcgtt tctacatcgc cctgttcgac aatcttaccg tcagacatca ccgccacgtc
     2221 atccgccaga cgcgccacaa cgcccatatc atgggtcacc agcagcattc ccggcgcttg
     2281 tttttgcata atgctttcca gcagatcgag gatgcgcgcc tgtgctacca cgtcgaggtc
     2341 ggtggtcggt tcatcggcga tgataaacgg tgattcacac agcaccgcca tcgcaatcat
     2401 catgcgctgc aacatgccgc cgctcatctc gaacgggtac agcttcagca cgcgcgcggc
     2461 gttttccagc cccaccgctt ctatggcagc ggtaagcgta gcgtcatcgg cgggtttccc
     2521 taacgccagg caggtttccc gcgcgtgggt gtgcatggtg tgcagtggat taaaggcgct
     2581 gcgcgggttc tgcatgatgg tggcaatttt gatgccgcgc agggcgcaag gcgaaaccgg
     2641 tttgccatcg gctaaaattt cccccgccgt ctggcgaacg ccagcgggca aaatgcccag
     2701 cgtcgcggcg caggttaatg atttcccgct gccgctaccg ccgactaacg ccagcacgcg
     2761 cccgcgttgc agggttaacg atacaccgtg taccagcggc tgcgcggcct gtagcgcgat
     2821 attacgtagt tcaatctgtt gcggcattag tgtgcgtgct ccgtcaccag atgagggtcc
     2881 agatgatcgc gcagtgcgtc acccaccagg ttaaaggcca tcacgctgat aaacagcgcc
     2941 agccccggcc agaacatttg cagcggctgg gtccagatat actggcgcgc gtcgttaatc
     3001 atcacgcccc attcggcggt cggcgcggtc acaccgaggc caaggaaaga catccccgcg
     3061 acgtgcagca tcatatggcc gatatccagc gttgccagca ccagcagcga agggatcacc
     3121 gcgcctgcca gatgatcgac aaacacccgc acatggcccg caccggaaag ccgtgacgcc
     3181 agcacaaact cgcgttggcg tagtgaaatc accaggctgc gcaccatgcg tgcataccac
     3241 gcccagtgcg acagggcgat ggcgataatt acgttggtca gcccggtgcc gagcacgcca
     3301 accataaaga acgacagaat cgaggtcggg aaggtcataa acatatcggc gacgcgcatg
     3361 gtggcctgat caacgcgccc gccaatcaac ccggcgctgc cgccaataac cagccctaat
     3421 gtcagcacca gcagcaggca ggccattacc gaaccgagcg acacgcgggt cgctgtcatc
     3481 agccgcgaga aaatatcgcg acctaagtga tcggtgccca gccagtgctg cgcatccggc
     3541 gaaagcaggc gcgacggcaa atcaatcgcc tgggggtcat acggcagcca ccactggctg
     3601 gtgagcgcaa tcagcgccag cagggcgata atgatcagcg ccaggcgtac cgaccagcgg
     3661 gaagagagga aaaagttcac gcgtgcgctc cttcatgacg acgaatgcgc gggtccagcg
     3721 cggcgttgag caaatcgaca atcaaattac agaccacaaa aaccaccacc atcatcagcg
     3781 taaagcactg gatcaccgga tagtcacggt taaaaatcgc cgacaccgca tagcgcccga
     3841 cgcccggcca ggcaaagatg ttttcgataa tcatcgtccc gccaatcagt tcgccgatat
     3901 gcatccccac ggcggtgatc atcggcagcg aggcattgcg caggatgtgg cgacgttcgg
     3961 tctgtttgtc gttcaggccg cgcagacgcg cccaggtgac gtgacgctga ccggcgacgt
     4021 ccagcatact ggcgcgcagt aaacgcgcgt taatcgccag cgacataaag gcaatggaaa
     4081 ccgcaggcaa aatgatgtgc tgccagccgc cgtaacccat cgcgggtagc cattgcagat
     4141 acaccgaaaa cgccatcacc agtaaaaacg ccagccagaa gttaggcatc gacacgccaa
     4201 gaaacgcgat gaaacgtacg gcgaaatccg gcagacggtc gcgatggcgc gccgcccaga
     4261 taccgagcgg tacggaagtg agcagaatta ataccagcgc cgcacctgca agttccagcg
     4321 tggcgggcag gaagttcagc atatcgtcca gtaccgggcg ttggctggcg aatgagatac
     4381 caaagtcaag atgcagcgcc ttccacaacc aggtaccgta ctggacgtac agcggctgat
     4441 ccagcccgag catggtgcgg gtagaggcca gcatctccgg cgtcggcggc aggttagata
     4501 aacgcagata atcgagcgcc gggtcgccgg taccgaggcg cagcatcaga aaaatgatca
     4561 ccgaggcggc gagcaccatc gggatcagca gcagaaagcg gcgtaatacg taacgcaaca
     4621 ttaaggtttc accggtttaa tctgttcgaa cggaatttcg gtggcgatcg gcgcgtaggg
     4681 gatgttaccc agctccggtt ttgataccac catcattgag atgtaactga taggcagata
     4741 aaccgcctcg tcatgcagac gggtcagaat gtcgcgatac agcgcctgac gttgcgtttc
     4801 gtcatgggtc gccagcactt cgccgatctc tttatcaatc agcggtttgt cggctaatcc
     4861 ttgctgtgcc tggaagtcag cgtgtgacgg tacgcgcatt gaactgagga aggcgtgtgg
     4921 atcatatggc gcgccccagg tgcggtggaa aatcatgcca aaacgaccgt cgcgctgacg
     4981 agcatagata ctgctctctt cttcgccaat cagcgagaca tctgcgccaa tctggcgcat
     5041 atcagcctga atgatttccg ccatcgattt gcttaacgca tcggtgccga tgaacgacag
     5101 ttcaatgcgc agcggctgac catttttctc gcggatgtct ttgcccgcag gcagcgtcca
     5161 accggctttt tccagcaacg ctttcgcttt ctgcggatcg tactggcgcg gtttcaggcc
     5221 gaggttggcg tagggcacag aaggggcaaa cagggtgtcg gcgacctgct gggtgccata
     5281 caacgcgtta tcaatcagcg attttttgtt taccgcgtaa ttaagagctt cacgtactgc
     5341 cagctcgttg gtgggggctt tggcggtatt gagcgccagc atcacggttt cgatcggctg
     5401 tgacagttgg gtgtggtaag ccggattctg gctaaagcgg gcgaaggtat cgagcggtaa
     5461 taacccttcg tttccgtaca gcaggtcgat atcgccagtt tcaaacgcca ccgcgcgggt
     5521 agtcgggtcc gggatgacgt taaaggtgat ctttttaatc gctggctttt cgccccagta
     5581 gttttcgtta cggacgaaga catcgtactg attcagtttc gattcctgca aaatccacgg
     5641 tccggtgcca atcggcgctt taattccgtt catggtttca tggtttttaa actgcgaggg
     5701 agcgataaaa cggaaaggac ggggcagggc cagttcttgc aggaaaggat agtaggcgct
     5761 tttcagggta atttgcagct ctgttttact gagtgcttta acatcaacaa tctggtttgc
     5821 cagctccagc caggcgtgac gttgacggtt atcgagcact gcgcggaagt tttctgccgc
     5881 cgcctcggca tcgaacggtt caccgttgga gaatttcacg tcatcacgca gggtgaaggt
     5941 ccaggtttta ccatcttctg aatgagtcca gctttttgcc agccacggga tcaccgaacc
     6001 gtctgcctga tatttcacca atggttcata aaccatgctc tgggcgaaca tctggttagg
     6061 cgtgtaaagg tgcgggttta gtggcccgac attcaccggc caggcggtgg tgatttcatc
     6121 tggtgcagcg gcatggacga taaaagacgc acaagccagc agcgcaaata gagtgcggcg
     6181 gagtgtggag agcatggtaa ccccaatgga ttaaaataga tggcggaaat aagtatgacg
     6241 attttaagta ttcgtcatac tgattacctg ttctggatca atagatgggc agtcgggtgg
     6301 gcggatcagt taactgaatc gatccattgc acactgtcgg cggtgagcgt aaagggagta
     6361 ggggtgcaga tcgccaggct taaattttca agctgacaat ggctgactga cagcgaggag
     6421 tgataggtgc tgtctacgct gacgatttgc caggcgctgc cgccacgctg tttaacgatg
     6481 gcttctttgc gcgtccagat gcgccagaac atttccagtt gctgatcagg atgcacggcg
     6541 tccatctcag cgtgttcccc gaggctgaat acggcgttcg ccagccagtg ccagttggcg
     6601 cgcgggcgaa tcacttcgat atcgcagccg acttcgcctt catcactcaa cagcagggcg
     6661 atatcgtcac cgctatggct taagttgaac catagcggca tttccggcgc aaatgcaggt
     6721 ttgccttgtt cgccatagat gatctccggt agcggggaaa gcgtgtgcga aagcaatgca
     6781 cgccccgcca gccagcgttc gcgtcgtgga ccttgcggtg cttgctcgcg taaacccggt
     6841 ggcagtggag ctgcgcttaa ggtcgaaact ttccccagaa ctatccgata catatcaggg
     6901 ccaacgttta atggaaaatg aaagtgcgta tcgtatcact tgtcgcctca tcccggtaac
     6961 cgacttttcg gtctgcctgg ccccagtaaa atcgccagtt tgctaccgcc tttggtggtt
     7021 tccatccaga ttttacacac actggttaat ggcaccgaaa gtagcatccc taccgggccg
     7081 agcagccatc cccaaattaa caatgaaaga aataccacca tggtggacat ccccaggcga
     7141 tggcccatca tccgtggttc taaaatattg ccgatgacca tatggaccac taaaaacaat
     7201 gcgccgacca gaatacattc gtaaacacca ttaaacagca gcacctgaat cattggcggt
     7261 acggcggaaa ttaccgcgcc gatattgggc acgtagttga gcaaaaacgc cagtactgcc
     7321 cacatcagcg caaactgcac ccccatcagc tccagcccca gccagacgat gacacctgtc
     7381 cataaactga gtagcgtctt caatgcaaga tagtgcgaaa cgcctttaag tgcgcggtgt
     7441 aatcctgcga tgtgaatctg tgggttattc agcgcaaaac gcattttgta agggacgtgg
     7501 cgcacttcaa acagcataaa aactacggtc atcaccagca aaagcacgct cgccattgcc
     7561 ccggaaagcc cggtcattag cgctgtggtg aaggtaacca ctttttccga gtccatccgc
     7621 tgcagcattc gctccggcga catatgcaaa ttaagaaaag gcaacatctc ctgcaattta
     7681 aaaagtttgc gcgtcaactc cttattaaac ttcggcagca tagagataaa ttcgttaaac
     7741 gatgccgcca gtacgccgac cagcgcagtt agtgcgatca gcatcaccac caccacaatc
     7801 gtaatggcaa tggggcgttg tactccccga cgaataaacc aggtgacgag cgggttgagg
     7861 acgatggcaa aaaacagcgc cagtaacagc tgcacaatga tatctgccgc tgcgtgaatg
     7921 cccgcgagga tcactaccag cgaggccagc ttgagcagaa tgtgcatgcc cgttttatcg
     7981 ggttgagggg tttccattgg ggcttccttg tgactttttg tattaagtgt agcgggagtc
     8041 acgccagcaa tattctgatt cttactgctg attttcacgt cagcacgtgg taaaaatgaa
     8101 acactgttgt aaaaatgtgg tgatcctcat gcccgaaccc gtagccgaac ccgcgctaaa
     8161 cggattgcgc ctgaatttgc gcattgtctc cattgtcatg tttaacttcg ccagctacct
     8221 caccatcggg ttgccgctcg ctgtattacc gggctatgtc catgatgtga tgggctttag
     8281 cgctttctgg gcaggattgg ttatcagcct gcaatatttc gccaccttgc tgagccgtcc
     8341 tcatgccgga cgttacgccg atttgctggg ccccaaaaag attgtcgtct tcggtttatg
     8401 cggctgcttt ttgagcggtc tgggatatct gacggcagga ttaaccgcca gtctgcccgt
     8461 catcagcctg ttattacttt gcctgggacg cgtgatcctt gggattgggc aaagttttgc
     8521 cggaacggga tcgaccctgt ggggcgttgg cgtggttggc tcgctgcata tcgggcgagt
     8581 gatttcgtgg aacggcattg tcacttacgg ggcgatggcg atgggtgcgc cgttaggcgt
     8641 cgtgttttat cactggggcg gcttgcaggc gttagcgtta atcattatgg gcgtggcgct
     8701 ggtggccatt ttgttggcga tcccgcgtcc gacggtaaaa gccagtaaag gcaaaccgct
     8761 gccgtttcgc gcggtgcttg ggcgcgtctg gctgtacggt atggcgctgg cactggcttc
     8821 cgccggattt ggcgtcatcg ccacctttat cacgctgttt tatgacgcta aaggttggga
     8881 cggtgcggct ttcgcgctga cgctgtttag ctgtgcgttt gtcggtacgc gtttgttatt
     8941 ccctaacggc attaaccgta tcggtggctt aaacgtagcg atgatttgct ttagcgttga
     9001 gataatcggc ctgctactgg ttggcgtggc gactatgccg tggatggcga aaatcggcgt
     9061 cttactggcg ggggccgggt tttcgctggt gttcccggca ttgggtgtag tggcggtaaa
     9121 agcggttccg cagcaaaatc agggggcggc gctggcaact tacaccgtat ttatggattt
     9181 atcgcttggc gtgactggac cactggctgg gctggtgatg agctgggcgg gcgtaccggt
     9241 gatttatctg gcggcggcgg gactggtcac aatcgcgtta ttactgacgt ggcgattaaa
     9301 aaaacggcct ccggaacacg tccctgaggc cgcctcatca tcttaaaact tactgaataa
     9361 ccagcgtatt aatgatgttt tctgcggtgg tctgcgcttt ttgctgatcg tcagctggca
     9421 gagtaatttg catggtcagc agttgattac ccacgttacc cagaataacg gaagagtacg
     9481 ccgtctggcc tttcgcggag ataatactgt ctaactgctg cattttgtga cctttcagct
     9541 caatggcttt attggttacc acttgcagct gcggatcgcg gctacgttgc tgatcttcca
     9601 gacgcttcgc cagcaccgcc agatcttctt ttggatcatc gcccatgatg acgatgactg
     9661 ctttctgccc ggtggcgtcg gaccagacat gcatgttatt ggcctgcgtt cccagcttac
     9721 cgctctggtc ggtcatatcc gctggcagcg agaaacttaa cttgccatca agcaggttga
     9781 cgggattccc ggtagcgtta ctttccgcga ccgaaccctg cgccgtagcg ttagtgtctt
     9841 tatcatcaca ggccgcaagc cccataacca gcaggccaat tccgacatat ttaaccagat
     9901 tgcgcattga cttcttcctt tcgataaacg gccataacgg ctcattcatc catcttatca
     9961 caactctgat aacgaacctt taactcgcct gcaaagcgtt gatttcggat ttatctgcca
    10021 gtcttttcaa cagcatattg agtaatacgc catacattgg caggaagaaa acgatactga
    10081 ttaacacttt gaaacagtaa tcgaccagcg cgatttccat ccagtgttca gccataaagg
    10141 catccgggct acgccagaag gcaatgaaga aaaaggccag cgtgtcgctg acgttaccga
    10201 acagtgtgga cgctgtcggt gccagccacc agcggcgact ttgacgcagg cggttaaaaa
    10261 cgtgcacgtc gaggatttgc cccagcgcgt aggccatgaa actggcggtg gcgatacggg
    10321 cgacaaacag gttgaagtgg gcgagtgcgc cgaatccctg ccatgaaccc atatagaaca
    10381 gcgacgagat gacataggag attaataacg cagggatcat taccgcgaag ataatgcgtc
    10441 gggccagcgg tgcgccaaaa atacgcacgg tcaggtcggt agcaagaaaa ataaacggaa
    10501 agctaaacgc gccccaggtg gtatggaaac caaaaatgga gacgggaagc tgaaccagat
    10561 agttactgga ggtgatcacc agcagatgaa ataacgataa ccagaacaac gccttatagc
    10621 gttgagtttg cgagaaaacg ttcatattgt acctttttga ttaaccattg gggtgaggga
    10681 acccaatacg tacgacacgt ctgttacctt aacataataa cgtgcttacc cttttttcga
    10741 gccgccgcat gatactgctt tacgttgaca atgcaatggt taattttcac gcaatcgtta
    10801 acctggtttg cttacggact cacagggcgt aaactagcgc cgttttttta tgtgatgaga
    10861 agaaaatgac cgatctcttt tccagccctg accacacact cgacgcactt ggcctgcgct
    10921 gcccggaacc cgtaatgatg gtgcgcaaaa ctgtgcgcaa tatgcagccc ggcgaaacgt
    10981 tgctgattat cgccgacgat ccggccacta cccgcgatat tcctgggttt tgtaccttta
    11041 tggaacacga actggttgcg aaagagacgg atggactgcc ttatcgttat ttgattcgta
    11101 aaggcggttg atgggggctg attggcttcg atgccgcctt ttcccctcac cctaaccctc
    11161 tccccagagg ggcgagggga ccgatcgcgc tcaatgttgc gatcggtttg ccttatctcc
    11221 tgcgcaacaa tcttaacgca ttcgctgtca ccagcaccgt cgcacccgta tctgccagca
    11281 ccgccagcca tagtccggtc atccctaaca gcgtggtgac gaggaagatc cctttcagtc
    11341 ccagcgcaat ggtgatgttc tggcggatat tggcgtgagt ggcgcgtgcc agttcaatca
    11401 tttgcaccag gccgcgcagg tggttatggg ttaatgctgc atcggcggtt tccagcgcca
    11461 cgtctgtgcc gctgcccatt gcaatcccaa tggcggcagc tttcatcgct ggcgcgtcgt
    11521 taataccatc accgaccatc gccagcggcg catgttgatt cagcttggtc accgctttga
    11581 ctttctcttc cggcaatagg cccgctttaa actccagccc cagctccccg gcgattgccg
    11641 ccgctgcgcg tggattatcg ccggtgagga tcactccttt aactcccagc gcgttcagtt
    11701 cactgatggc agttgcagcg tcggcgcgca gggtatcctg caatgcaatg atacccagca
    11761 cgtcatcatt acgtactacc agcacaaccg tttgcccggc gctttccagt tcattaatca
    11821 aaccagcaaa tgcatcagcg ggatgtttcc cggcagcgca aatcaatacg cgctcaccgt
    11881 taacctgcgc ttcaatgcca gacccgacca gcgcccgctg tgattcggcg gtgggaatgg
    11941 cgagttcagc aacctgtgct tctcgcacga tggcctgcgc cagtggatgc gtcgcacctt
    12001 gctcgaccgc cgctgccagt gtcagcagtt cagattcact aatacccgtt gccggatgaa
    12061 tcgcggtaac gcgcggttta ccgacggtca gcgtaccggt tttatcaaac gccacctggg
    12121 taacacgacc tagttgttcc agcgccgctc cgcctttaat taacgcccca cgacgcgctg
    12181 ctgccgccag cccggaggta atcgccgcgg gcgtggagat aactaacgca cacgggcagc
    12241 caatcagcag cagcgtcagg cctttataaa tccactcttg ccagctggcg gcaaacagca
    12301 gcggtggcac cagcgtcacc agcagagcga cggccataat cgcgggcgta tagatacggc
    12361 tgaaacggtc gataaaccgc tcaatgggtg cgcgacgctc ttcggcttct tcgatcagtt
    12421 tcagaatccg gtcaatggcg ctggcacccg gttctgacag cacttccagc gttaccagac
    12481 ggtctacgct ggtggctcca gcagggactt tatcgcccgt tgcgcgctcc accggaatag
    12541 attcgccggt cagggcgctt tcatcaaaac tggcaaacgg tgagagcagt ttaccgtcgg
    12601 caggcaaacg tccacctgcg gcgacttcaa tcacatcgcc agggcgcagg ctgttaatcg
    12661 ccacctcttc ccgctcaccg ttacgcaggc gcgtggcggt ttctggtttc agcgccatta
    12721 acgcgctgac cccttgacgt gcgcggctgg cggcccagcc ttccagtcgt tcgccaatca
    12781 aaaacagcag caacaccatc gcagcttcag ccgttgcgcc aataaaaagc gcaccaatag
    12841 cggctacgct cattaaggtt tcaatggcga agtagctgcc ggatttgatc aaccgtaatg
    12901 cctgacgagc aatcgggtac agcccaacca gtgtggtcgc gataaacgcc agttgcccga
    12961 acggatgatt gaactgctcc agaccccagc tgattgccat catcacgatt agcgtaatca
    13021 gcggcagatt ctctttcagg cgtgatgctt gcggttcgtc ggcggactgt tcatcgcgca
    13081 gggagtagcc cgctttttgc accgcagatt caacctgggc acggatgtca ttgtcggcat
    13141 caaccaccag tttttcggtg gcgaacaaca cctgtacctg attcacgcct gcaagctggc
    13201 gcacggcatt ttctaccttg cgcgcacagg cggcgcagtc catgccgctg actttccagc
    13261 tatagcgggt gccggagacg ttttcagaga gagttggcgt gctggaacat gcgccgtcgc
    13321 agcaacagtc gttggcgttc tgtaccgtgg ttagcggttt gaacgcagca aattgagggg
    13381 ctttcttgcc gtgattgtca ggagtcgaca tggcatcctc cggttaagtt ttttctcatt
    13441 aaccgaagga tacactctgg agtcgactcc agagtcaagt tttatcagag atacagcgag
    13501 cggacgatca ggaagtgacc ggcaaagtag caggccgcag caatcgcgtt atccgcacgg
    13561 aagcgtcggc gatagtgact acccagccag acaaagttac tgataaacag caacgaagcg
    13621 ccgacaaacg cagagagcgc cggagcggtc ggacggaaga accacagttc acctgccagc
    13681 cacaccatca ccagcgtcat gccgataaac gtgcagatag gccaacgata ctcttccagc
    13741 cgcgtccaga taatcgccag taacagcgcg cccagaacca gcagcaccag tggcagaggc
    13801 cagaagaaag agagcgtcat ctgactggcg aaatagatgg tgtacagcag gtgcgagagg
    13861 aaaaacgcgc cgatggcgta catcagacgt tgacgtggca acagggttag cgcatcgccc
    13921 agcagtgagg cgcacagccc cgccagcacc agatagctaa tggcgtcgaa catcggcgct
    13981 tgccaggcca gtaacagcag gagaagaagg gttaatggtt taaataccca gcgttgccag
    14041 gttggcccac gatacgatgc atccacagat agccatgcgg aaagacagac agcgataaac
    14101 gaccaaagca tcttagttcc ttgaattcgt tattttttcg taagcggaat gcctacggtc
    14161 ttgcttccag tttagtggcg agggtggacg gatgacaaca ccataactgg cagggtatgc
    14221 ttaattccgc attttccgat gagggataaa gatgagcaaa ccacctcttt tctttattgt
    14281 tatcattggc ttaatcatcg tcgccgcatc gtttcgtttt atgcaacaac gacgggaaaa
    14341 agctgataat gatatggcac cactccagca aaagctggtg ttggtgagca acaagcggga
    14401 aaaaccgatt aacgatcgcc gttcgcgcca gcaggaagtg actccggcag gcaccagtat
    14461 gcgctatgag gcaagcttca aaccgcaaag cggaggaatg gagcagacgt ttcgcctcga
    14521 cgcccagcag taccacgccc tgacagtggg cgataaaggt acgctgagct ataaaggaac
    14581 gcgctttgtc agctttgtag gcgaacaata aggttatttt ttaactttaa atttcttctg
    14641 ccagaccagc agttcaaaca cgccgaaaag gaaaatccgt accttttcgg cggtggtcat
    14701 ctgcgggcca tctttcggta aagtggattt caatagcgcc agctgcatac catgcatcag
    14761 cacggtaaaa atcagcgcca cgttaacgaa aatattcagc ggacgtggga agggatgcac
    14821 caggttgagg attaaaaatc cccaaacaca gagcattaac aaacgaccaa tattaatcag
    14881 catcactttc tccttgtgct tcgcgttgat acagccgata agccacctga cccgccactt
    14941 tttcccgatg taatgaccag tttgccggaa cagtgggcag accgttttcg acttcgcttt
    15001 cgacataaat cagggcttcg tcagccagcc agccgttatc ttccagtaaa tttatcgtct
    15061 cttctaacaa gccacggcgg aacggtggat cgacaaacac gatattatgc ggtgtacctt
    15121 tttgcgccag gaatgacatc gcgttgctgt tcaccacgcg tgcattgcct gcttttagtg
    15181 tcgccagatt cttaattaac tgctgagaaa ccgcgcgatc catctcaatc aacgttgccc
    15241 ccgcagcata gcgcgacaac gcttccagcc ccagcgcgcc gcttccggcg aagcaatcca
    15301 gacattgggc gtcaacaatg accggagcca gccagttaaa caacgtttcg cgtacgcggt
    15361 cggtggtggg gcgcagacct gggctatcag gaaccgggag tttacggcct cgccactgcc
    15421 cgccaataat gcggatttgg ccgctgccgg aatgattcgg ttttttcatg ataaattgct
    15481 caatccgcca gataacacat acttgcaggc ggtgatgtga attaagttaa gtgatagact
    15541 atttcatcat ttttttagct gctatgtaca tagcgttaac gctgtgccat gaagcaacag
    15601 cgaggagtgt agtcgcaaat ggcgaaagaa aaaaaacgtg gctttttttc ctggctgggc
    15661 tttggtcaaa aagagcagac cccggaaaaa gagacagaag ttcagaatga acaaccggtt
    15721 gtagaagaaa tcgttcaggc gcaagagcct gtgaaggctt cggaacaagc cgttgaagag
    15781 cagccgcagg cgcatactga agccgaggcg gaaacttttg ctgccgacgt tgtggaagtc
    15841 actgaacagg ttgctgaaag tgaaaaagcg cagcctgaag cggaagtcgt tgcacagccg
    15901 gaaccggtcg cagaagaaac gccggagcca gtggctatcg aacgtgaaga gctgccgttg
    15961 ccagaagacg tcaacgccga agaggtttcg ccagaagagt ggcaggctga agcggaaacc
    16021 gtagagattg tcgaagcggc agaagaagaa gcggctaaag aagaaatcac cgacgaagag
    16081 ctggaagcac aggcgctggc tgccgaagcg gcagaagagg cggtgatggt agttcctccg
    16141 gcagaagaag agcagccggt ggaagaaatt gcccaggagc aagaaaaacc gaccaaagaa
    16201 ggtttcttcg cgcgcctgaa acgcagcctg ttaaaaacca aagaaaatct cggttccgga
    16261 tttatcagcc tgttccgcgg taaaaaaatc gacgatgatc tgtttgagga gctggaagaa
    16321 cagctgttga tcgccgatgt gggcgtggaa accacacgta aaattatcac caatctgacg
    16381 gaaggcgcat cccgcaagca gcttcgtgat gccgaggcgc tctatggcct gctgaaagaa
    16441 gagatgggcg agattctggc gaaagtcgat gagccgctga atgttgaagg taaaacgccg
    16501 ttcgtgatcc tgatggtggg cgtcaacggt gtgggtaaaa ctacgacgat tggtaagctg
    16561 gcacgtcagt ttgagcagca gggtaaatcg gtgatgctgg cggcgggcga tactttccgt
    16621 gcagcagcgg ttgaacagct tcaggtctgg ggtcagcgca acaatattcc ggtgattgcc
    16681 cagcataccg gtgcggattc cgcctctgtt atcttcgacg ccattcaggc agctaaagcg
    16741 cgtaatatcg acgtcctgat tgccgatacc gccggacgcc tgcagaacaa atcgcacctg
    16801 atggaagagt tgaagaaaat cgtccgcgtg atgaagaaac tcgacgttga agcgccgcat
    16861 gaagttatgc tgactattga tgccagtacc gggcagaatg cggtaagcca ggccaaactg
    16921 ttccacgaag ccgttggctt aaccggcatc acgctaacga aactggacgg cacggcgaaa
    16981 ggcggggtaa ttttctcggt ggctgaccag tttggtatcc ctatccgcta cattggtgtc
    17041 ggcgaacgta ttgaggattt gcgtccgttt aaggcggacg actttataga ggcacttttt
    17101 gcccgagagg attaacaatg attcgctttg aacatgtcag caaggcttat ctcggtggga
    17161 gacaggcgct gcagggcgtt acgttccata tgcagccggg tgagatggcg tttctgaccg
    17221 gtcattccgg cgcagggaaa agtaccctcc tgaagctgat ctgtgggatt gagcggccca
    17281 gcgccgggaa aatctggttt agcggccatg acatcacgcg tctgaaaaac cgtgaagttc
    17341 cgtttctgcg ccgccagatt ggcatgattt tccaggatca ccatctactg atggaccgta
    17401 ctgtctacga taacgtggcg atcccgctga ttatcgctgg tgccagcggt gacgatattc
    17461 gtcgccgggt gtcggcggca ctggataaag tcgggctact ggacaaagcg aagaacttcc
    17521 ctattcagct ttccggcggt gaacaacagc gtgttggcat tgcccgcgcg gtggtgaata
    17581 agcccgcggt actgctggcg gacgaaccga ccggtaacct ggacgacgcg ttgtcggaag
    17641 ggattctacg tctgtttgaa gagtttaacc gcgttggggt aaccgtattg atggcaacgc
    17701 acgacatcaa cctgatttca cggcgttcct atcgcatgct caccctgagc gatggtcact
    17761 tgcatggagg cgtgggccat gaataagcgc gatgcaatca atcatattcg gcagtttggc
    17821 gggcgtcttg atcgcttccg taaatcggtc ggcggctctg gcgacggcgg tcgtaacgcc
    17881 ccgaaacgcg cgaaatcctc gccaaaaccg gtaaatcgta aaaccaacgt tttcaacgaa
    17941 caggtgcgct atgccttcca cggcgcattg caggatctga aaagcaaacc gttcgccacg
    18001 tttttaacgg tgatggttat cgccatctcc ctgacgctgc ccagcgtctg ttatatggtg
    18061 tacaaaaacg ttaatcaggc ggcgacgcag tattatccgt caccgcaaat cactgtttac
    18121 ttgcaaaaaa cgctggacga tgacgccgct gcgggcgtgg tggcacagtt gcaggccgag
    18181 caaggcgtgg agaaagtgaa ctatctttct cgtgaagacg cactgggcga gttccgtaac
    18241 tggtctggtt ttggtggtgc gctggatatg ctggaagaaa acccgcttcc ggcagtggcg
    18301 gtggtgatcc cgaaactcga ttttcagggg acggaatcac tcaatacgct gcgtgatcgt
    18361 atcacgcaga ttaacggcat tgacgaagtg cggatggatg acagctggtt tgcccgtctg
    18421 gcggcgttga ccgggctggt cgggcgcgtt tcggcgatga tcggcgtgtt gatggtggcg
    18481 gcagtgttcc tcgtcatcgg caacagcgta cgtctgagca tctttgctcg ccgtgactcc
    18541 atcaacgtgc agaaactgat tggtgcgacg gatggtttca tccttcgccc gttcctgtat
    18601 ggtggcgcac tgctgggatt ttctggcgca ttgttgtcat taattttgtc agaaattctg
    18661 atgctgcgat tgtcatcggc ggttgcggaa gtggcacagg ttttcggaac gaagtttgat
    18721 atcaatggtt tatcgttcga tgaatgcctg ctattgctgc tggtatgctc gatgattggc
    18781 tgggtggcag cgtggcttgc cacggtacaa catttacgcc actttacgcc tgaataataa
    18841 aagcgtgtta tactctttcc ctgcaatggg ttccgtagca gggaaagaga tcccgttgtc
    18901 tcttcccggt atttcatctc tatgtcacat tttgtgcgta atttattcac aagcttgcat
    18961 tgaacttgtg gataaaatca cggtctgata aaacagtgaa tgataacctc gttgctctta
    19021 agctctggca cagttgttgc taccactgaa gcgccagaag atatcgattg agaggatttg
    19081 aatgactgac aaaatgcaaa gtttagcttt agccccagtt ggcaacctgg attcctacat
    19141 ccgggcagct aacgcgtggc cgatgttgtc ggctgacgag gagcgggcgc tggctgaaaa
    19201 gctgcattac catggcgatc tggaagcagc taaaacgctg atcctgtctc acctgcggtt
    19261 tgttgttcat attgctcgta attatgcggg ctatggcctg ccacaggcgg atttgattca
    19321 ggaaggtaac atcggcctga tgaaagcagt gcgccgtttc aacccggaag tgggtgtgcg
    19381 cctggtctcc ttcgccgttc actggatcaa agcagagatc cacgaatacg ttctgcgtaa
    19441 ctggcgtatc gtcaaagttg cgaccaccaa agcgcagcgc aaactgttct tcaacctgcg
    19501 taaaaccaag cagcgtctgg gctggtttaa ccaggatgaa gtcgaaatgg tggcccgtga
    19561 actgggcgta accagcaaag acgttcgtga gatggaatca cgtatggcgg cacaggacat
    19621 gacctttgac ctgtcttccg acgacgattc cgacagccag ccgatggcac cggtgctcta
    19681 tctgcaggat aaatcatcta actttgccga cggcatcgaa gatgataact gggaagagca
    19741 ggcggcaaac cgtctgaccg acgcgatgca gggtctggac gaacgcagcc aggacatcat
    19801 ccgtgcgcgc tggctggacg aagacaacaa gtccacgttg caggaactgg ctgaccgtta
    19861 cggtgtttcc gctgaacgtg tgcgccaact ggaaaagaac gcgatgaaaa aacttcgcgc
    19921 cgctattgaa gcgtaatttc cgctattaag cagagaaccc tggatgagag tccggggttt
    19981 ttgttttttg ggcctctaca ataatcaatt ccccctccgg caaaacgtca atccccacgc
    20041 agattgttaa taaactgtca aaatatctat tccaatatca taaaaatggg gtatgtttta
    20101 gcagagtatg ctgctaaagc acgggtagtc atgcataaaa cgaaataaag tgctgaatca
    20161 caacaccaca acacacgtaa taaccagaag aatggggatt ctcaggatga atataaaggg
    20221 taaagcgtta ctggcaggat gtatcgcgct ggcattcagc aatatggctc tggcagaaga
    20281 tattaaagtc gcggtcgtgg gcgcaatgtc cggtccggtt gcgcagtacg gtgaccagga
    20341 gtttaccggc gcagagcagg cggttgcgga tatcaacgct aaaggcggca ttaaaggcaa
    20401 caaactgcaa atcgtaaaat atgacgatgc ctgtgacccg aaacaggcgg ttgcggtggc
    20461 gaacaaagtc gttaacgacg gcattaaata tgtgattggt cacctctgtt cttcatcaac
    20521 gcagcctgcg tctgatatct acgaagacga aggcatttta atgatcaccc cagcggcaac
    20581 cgcgccggag ctgaccgccc gtggctatca gctgatcctg cgcaccaccg gcctggactc
    20641 cgaccagggg ccgacggcgg cgaaatatat tcttgagaaa gtgaaaccgc agcgtattgc
    20701 tatcgttcac gacaaacagc aatacggcga aggtctggca cgagcggtgc aggacggcct
    20761 gaagaaaggc aatgctaacg tggtgttctt tgatggtatc accgccgggg aaaaagattt
    20821 ctctacgctg gtggcgcgtc tgaagaaaga gaatatcgac ttcgtttact acggcggtta
    20881 ccacccggaa atggggcaaa tcctgcgtca ggcacgcgcg gcagggctga aaactcagtt
    20941 tatggggccg gaaggtgtgg ctaacgtttc gctgtctaac attgcgggcg aatcagcgga
    21001 agggctgctg gtgaccaagc cgaagaacta cgatcaggtt ccggcaaaca aacccattgt
    21061 tgacgcgatc aaagcgaaaa aacaggaccc aagcggcgca ttcgtttgga ccacctacgc
    21121 cgcgctgcaa tctttgcagg cgggcctgaa tcagtctgac gatccggctg agatcgccaa
    21181 atacctgaaa gcgaactccg tggataccgt aatgggaccg ctgacgtggg atgagaaagg
    21241 cgatctgaaa ggctttgagt tcggcgtatt tgactggcac gccaacggca cggcgaccga
    21301 tgcgaagtaa tcattaatcg gcaactttgg gttgccgcca aattgctaat atcgagtacg
    21361 ttgcttcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaagatcc aaagaaatca
    21421 gtaaattgca acacacattg taggcctgat aagcgtagcg catcaggcaa tttctcgttg
    21481 gtcatcactt ttgaaatcaa cttgacgatt aacgcttctc ccagccgccc tgttgcgccg
    21541 taaaccccag cgcctgcata aacgccgtca tcacaccgcg atcttccaag cctgcatccg
    21601 ccatccacca gcatgaaacg ccaggattgt tacgcaaaac ctcttccagc agatattgcc
    21661 ccacaccgcg acgtcgggtg acttcccgca cacgcaggga atccagtgct ccctcggtgc
    21721 cgcttaaggt tacccgtacg gcagcgagca ggcgctcgtt aaaacgcgcg gcgtagatac
    21781 ggtggttatc gtcaacctgt aaagaggaag gggaatactc cggccagatc ttttgcaggt
    21841 caatccggtc ctggtcgcta aagttttcta atcgaatgat ggtcagcttc atgggtaacc
    21901 cgtgtaaatc acaaaagtgg aaccagtgta gcgaaataat ttaatcggag gctttctctt
    21961 ttttatttct tttggcaggt gattaatttt ttaacagcaa taattacaaa gttaaaacat
    22021 tagagaatga aaaataccca gcataatccc ctgaatggta gtgaattatt ccgccctttg
    22081 tgccgttatt ttatgctgac aaaggcactt ttttctgttt atctatcaat aaattcagaa
    22141 tattatctgt tcttaatcga ctgaaaaata gggattttaa tcactattat cacaaaatac
    22201 tgcgctaaac ccttaatcag acaggcaaaa acagtgcagt ataaaaaaag aacagtctga
    22261 tttgttaaca cataaaaaca aagcaacaca acatcacgaa tggggatttt tgactatgaa
    22321 acggaatgcg aaaactatca tcgcagggat gattgcactg gcaatttcac acaccgctat
    22381 ggctgacgat attaaagtcg ccgttgtcgg cgcgatgtcc ggcccgattg cccagtgggg
    22441 cgatatggaa tttaacggcg cgcgtcaggc gattaaagac attaatgcca aagggggaat
    22501 taagggcgac aagctggttg gcgtggaata tgacgacgcc tgcgacccga aacaagccgt
    22561 tgcggtcgcc aacaaaatcg ttaatgacgg cattaaatac gttattggtc atctgtgttc
    22621 ttcttctacc cagcctgcgt cagatatcta tgaagacgaa ggtattctga tgatctcgcc
    22681 gggagcgacc aacccggagc tgacccaacg cggttatcaa cacattatgc gtactgccgg
    22741 gctggactct tcccaggggc caacggcggc aaaatacatt cttgagacgg tgaagcccca
    22801 gcgcatcgcc atcattcacg acaaacaaca atatggcgaa gggctggcac gttcggtgca
    22861 ggacgggctg aaagctgcta acgccaatgt tgttttcttc gacggcatta ccgctggcga
    22921 gaaagatttc tccgcgctga tcgcccgcct gaaaaaagaa aacatcgact tcgtttacta
    22981 cggcggttac tacccggaaa tggggcagat gctgcgccag gcccgttccg ttggcctgaa
    23041 aacccagttt atggggccgg aaggtgtggg taatgcgtcg ctgtcgaata ttgctggcga
    23101 tgctgccgaa ggcatgttgg tcaccatgcc aaaacgctat gaccaggatc cggcaaacca
    23161 gggcatcgtt gatgcgctga aagcagacaa gaaagatccg tccgggcctt atgtctggat
    23221 cacttacgcg gcggtgcaat ctctggcaac agcccttgag cgtaccggca gcgatgagcc
    23281 gctggcgctg gtgaaagatt taaaagctaa cggtgcaaac accgtgattg ggccgctgaa
    23341 ctgggatgaa aaaggcgatc ttaagggatt tgattttggt gtcttccagt ggcacgccga
    23401 cggttcatcc acggcagcca agtgatcatc ccaccgcccg taaaatgcgg gcgggtttag
    23461 aaaggttacc ttatgtctga gcagtttttg tatttcttgc agcagatgtt taacggcgtc
    23521 acgctgggca gtacctacgc gctgatagcc atcggctaca ccatggttta cggcattatc
    23581 ggcatgataa acttcgccca cggcgaggtt tatatgattg gcagctacgt ctcatttatg
    23641 atcatcgccg cgctgatgat gatgggcatt gataccggct ggctgctggt ggcggcggga
    23701 ttcgtcggcg caatcgtcat tgccagcgcc tacggctgga gtatcgaacg ggtggcctat
    23761 cgcccggtgc gtaactctaa gcgcctgatt gcgctcatct cggcaatcgg tatgtccatc
    23821 ttcctgcaaa actacgtcag cctgaccgaa ggttcgcgtg acgtggcgct gccgagcctg
    23881 tttaacggtc agtgggtggt gggccatagc gaaaacttct ctgcctctat tactaccatg
    23941 caggcggtga tctggattgt caccttcctc gccatgctgg cgctgacgat tttcattcgc
    24001 tattcccgta tggggcgcgc gtgtcgtgcc tgcgcggaag atctgaaaat ggcgagtctg
    24061 cttggcatta acaccgaccg ggtgattgcg ctgacctttg tgattggcgc ggcgatggcg
    24121 gcggtggcgg gtgtgctgct cggtcagttc tacggcgtaa ttaaccccta catcggcttt
    24181 atggccggga tgaaagcctt taccgctgcg gtgctcggtg ggattggcag cattccaggg
    24241 gcgatgattg gtgggctgat cctggggatt gcggaggcgc tctcttctgc ctatctgagt
    24301 acggaatata aggatgtggt ttcatttgcc ctgctgattc tggtgctgct ggtgatgccg
    24361 accggtattc tgggtcgccc ggaggtagag aaagtatgaa accgatgcat attgcaatgg
    24421 cgctgctctc tgccgcgatg ttttttgtcc tggcgggcgt ctttatgggc gtgcaactgg
    24481 agctggatgg caccaaactg gtggtcgaca cggcttcaga catccgttgg cagtgggtgt
    24541 ttatcggcac ggcggtggtc tttttcttcc agcttttgcg accggctttc cagaaagggc
    24601 taaaaagcgt ttccggaccg aagtttattc tgcccgcgat tgatggctcc acggtgaagc
    24661 agaaactatt cctcgtggcg ctgctggtgc ttgcggtggc gtggccgttt atggtgtcgc
    24721 gtgggacggt ggatatcgcc accctgacca tgatctacat tatcctcggc ctcgggctga
    24781 acgtggttgt tggtctttct ggtctgctgg tgctggggta cggcggcttt tacgccatcg
    24841 gcgcttacac ttttgcgctg ctcaatcact attacggcct gggcttctgg acctgtctgc
    24901 cgattgccgg gttaatggca gcggcggccg gcttcctgct cggcttcccg gtgcttcgct
    24961 tgcgcggcga ctatctggcg atcgttaccc tcggtttcgg cgaaattgtg cgcatattgc
    25021 tgctcaataa caccgaaatt accggcggcc cgaacggaat cagtcagatc ccgaaaccga
    25081 cactcttcgg actcgagttc agccgtaccg ctcgtgaagg cggctgggac acgttcagta
    25141 atttctttgg cctgaaatac gatccctccg atcgtgtcat cttcctctac ctggtggcgt
    25201 tgctgctggt ggtgctaagc ctgtttgtca ttaaccgcct gctgcggatg ccgctggggc
    25261 gtgcgtggga agcgttgcgt gaagacgaga tcgcctgccg ttcgctgggc ttaagcccgc
    25321 gtcgtatcaa gctgactgcc tttaccataa gtgccgcgtt tgccggtttt gccggaacgc
    25381 tgtttgctgc gcgccagggc tttgtcagcc cggaatcctt cacctttgct gaatcggcgt
    25441 ttgtactggc gatagtggtg ctcggcggta tgggatcgca gtttgcggtg attctggcgg
    25501 caattttgct ggtggtatcg cgcgaattga tgcgtgattt caacgaatac agcatgttaa
    25561 tgctcggtgg tttgatggtg ctgatgatga tctggcgtcc gcagggcttg ctgcccatga
    25621 cgcgcccgca actgaagctg aaaaacggcg cagcgaaagg agagcaggca tgagtcagcc
    25681 attattatct gttaacggtc tgatgatgcg cttcggcggc ttgctggcgg tgaacaacgt
    25741 caatcttgaa ctgtacccgc aggagatcgt ctcgttaatc ggccctaacg gtgccggaaa
    25801 aaccacggtt tttaactgcc tgaccggatt ctacaaaccc accggcggca ctattttact
    25861 gcgtgatcag catctggaag gtttaccggg gcagcaaatt gcccgcatgg gcgtggtgcg
    25921 taccttccag catgtgcgtc tgttccgtga aatgacggtg attgaaaacc tgctggtggc
    25981 gcagcatcaa caactgaaaa ccgggctgtt ctctggcctg ttgaaaacgc cttctttccg
    26041 tcgcgcccag agcgaagcgc tcgaccgcgc cgccacctgg cttgagcgca ttggtttgct
    26101 ggaacacgcc aaccgtcagg cgagtaacct ggcctatggt gaccaacgcc gtctggagat
    26161 tgcccgctgc atggtgacgc agccggagat tttaatgctc gacgaaccgg cggcaggtct
    26221 taacccaaaa gaaaccaaag agctggatga gctgattgcc gaactgcgca atcatcacaa
    26281 caccactatc ttgttgattg aacacgatat gaagctggtg atggggattt cagaccgaat
    26341 ttatgtggtc aatcagggga cgccactggc aaacggtacg ccggagcaaa tccgtaataa
    26401 cccggacgtg atccgtgcct atttaggtga ggcataagat ggaaaaagtc atgttgtcct
    26461 ttgacaaagt cagcgcccac tacggcaaaa tccaggcgct gcatgaggtg agcctgcata
    26521 tcaatcaggg cgagattgtc accctcattg gcgcgaacgg ggcggggaaa accaccttgc
    26581 tcggcacgtt atgcggcgat ccgcgcgcta ccagcgggcg aattgtgttt gatgataaag
    26641 acattaccga ctggcagacg gcgaaaatca tgcgcgaagc ggtagcgatt gtcccggaag
    26701 ggcgtcgcgt cttctcgcgg atgacggtgg aagagaacct ggcgatgggc ggcttttttg
    26761 ccgaacgcga ccagttccag gagcgcataa agtgggtgta tgaactgttt ccacgtctgc
    26821 atgagcgccg tgttcagcgg gcaggcacca tgtccggcgg tgagcaacaa atgctggcga
    26881 ttggtcgcgc gctgatgagc aacccgcgtt tgctgctact tgatgagcca tcgctcggtc
    26941 ttgcgccgat tatcatccag caaattttcg acaccatcga gcagctgcgc gagcagggga
    27001 tgactatctt cctcgtcgag cagaacgcca accaggcgct aaagctggcg gatcgcggct
    27061 acgtgctgga aaacggccat gtggtgctct ccgataccgg cgacgcgctg ctggcgaacg
    27121 aagcagtgag aagtgcgtat ttaggcgggt aataacacgt tgattgataa ggagtcaaaa
    27181 gactcctttg agacaggtga caaatgtaaa attgcctgat gcgctgcgct tatcaggcct
    27241 actgggtgag ttgcaatata ttgaatttgc acgatcttgt aggccggata aggcgtttac
    27301 gccgcatccg gcatgaaacg atgggtaatc tgtagagttt gattcagacc ttctattttc
    27361 cgcttatccg tgccccatct cccattttcc ctcacccacg ccgtcaccgc cttgtcatct
    27421 ttctgacacc ttactatctt acaaatgtaa caaaaaagtt atttttctgt aattcgagca
    27481 tgtcatgtta ccccgcgagc ataaaacgcg tgaattcgcg cattcggtac aacaagagag
    27541 ataaacgatg aaaccgttac attatacagc ttcagcactg gcgctcggac tggcgttaat
    27601 ggggaatgca caggcagtga cgaccattcc gttctggcat tctatggaag gggaactggg
    27661 taaagaggtg gattctctgg cccaacgttt taacgccgaa aatccggatt acaaaattgt
    27721 accgacctat aaaggcaact acgaacagaa tttaagcgcg gggattgccg catttcgtac
    27781 cggcaacgct ccggctattt tgcaggttta tgaagttggc accgccacca tgatggcatc
    27841 gaaagccatt aaaccggtat atgacgtgtt taaagaggcg gggattcaat tcgatgagtc
    27901 gcagtttgtg ccgacggttt ccggttacta ctccgacagc aaaacgggcc acttactctc
    27961 ccagccattc aacagctcga cccccgttct ctattacaac aaagacgcct tcaagaaagc
    28021 aggattagac ccggaacaac cgccgaaaac ctggcaggat ctggcggact atgccgcgaa
    28081 actgaaagcc tccggtatga agtgcggcta cgccagcggc tggcagggct ggatccaact
    28141 ggaaaacttt agcgcctgga acggtctgcc gtttgccagc aaaaacaacg gctttgacgg
    28201 cacggacgcg gtgctggagt tcaacaagcc ggagcaggtg aaacatatcg ccatgctcga
    28261 ggagatgaac aagaagggcg acttcagcta cgtcggtcgt aaggatgaat ccaccgagaa
    28321 gttctataac ggtgattgcg cgatgaccac cgcctcttcc ggttctcttg ccaacattcg
    28381 cgagtacgcc aaatttaatt acggcgtagg catgatgcct tacgacgccg atgcgaaaga
    28441 tgcgccacaa aacgccatta tcggcggagc cagcctgtgg gtgatgcagg gtaaagataa
    28501 agaaacgtat accggtgtgg cgaagttcct cgatttcctc gcgaagccag aaaacgctgc
    28561 cgagtggcat cagaaaaccg gttatctgcc aattaccaaa gcggcgtatg acctgacccg
    28621 tgagcagggc ttttacgaga aaaacccagg tgcggatacc gcgacgcgtc agatgctgaa
    28681 taagccgccg ttgccgttca ccaaagggct gcgtctgggc aacatgccgc agatccgcgt
    28741 gattgtggat gaagagctgg agagcgtgtg gaccggtaag aagacaccac agcaggcact
    28801 ggataccgcc gttgagcgtg gaaatcagtt gctgcgccgc tttgagaaat cgacgaagtc
    28861 ttaatcagtg taatgtcgga tgcgtttcgc ttatctgact tggcatcgcg tgtaggccgg
    28921 ataagcgaag cgcatccggc acagttcagg aattaaccgt aatgtcatca tcccgtccgg
    28981 tgttccgctc gcgctggctg ccttatctgc tggtcgcgcc gcagctcatc atcaccgtta
    29041 tcttttttat ctggcctgcg ggcgaagcgt tgtggtactc gctacaaagc gtcgatccgt
    29101 ttggtttctc cagccagttt gtcggcctgg ataacttcgt cgcgctgttt catgacagct
    29161 actatctcga ctccttctgg acgacgataa aattcagcac ctttgtcacc gtcagcggtt
    29221 tgctggtgtc gctgttcttc gcggcgctgg tggagtacat cgtgcgcggc agccgtttct
    29281 atcaaacctt aatgctgctg ccttacgccg tggctcccgc cgttgccgcc gtattgtgga
    29341 tcttcctgtt taaccccggt cgcgggctga tcacccattt tctcgcggag ttcggctacg
    29401 actggaacca cgcgcaaaac agcggtcagg caatgtttct ggtggtgttt gcctcagtat
    29461 ggaagcaaat cagctacaac ttcctgttct tctatgccgc gctgcaatcc attccccgtt
    29521 cgttgatcga agccgcagcc atcgacggtg caggcccaat tcgccgcttc tttaagattg
    29581 cgctgccgct tatcgctccg gtgagtttct tcctgctggt ggtgaacctg gtgtatgcct
    29641 tcttcgacac cttcccggtg atcgacgccg ccacgtccgg cgggccggtt caggccacca
    29701 cgacgctgat ttataagatc taccgtgaag gctttaccgg actggatctg gcttcgtctg
    29761 ccgcgcagtc ggtggtgttg atgttcctcg tcatcgtgct gacggtggtg cagttccgct
    29821 atgttgaaag caaggtgcgt taccaatgat tgagaaccgt ccgtggctga cgatattcag
    29881 ccataccatg ctgatcctcg ggatcgcggt gatcctcttc ccgctgtacg tggcgtttgt
    29941 cgcggcgacg ctggataaac aggccgtcta tgccgcgccg atgacgctca tccccggcac
    30001 acatctgctg gaaaatatcc acaacatctg ggttaacggg gtaggcacga atagcgcgcc
    30061 gttctggcgg atgttgctta acagctttgt gatggcgttc agcattacgc tcggcaaaat
    30121 taccgtctcg atgctctcgg catttgccat tgtctggttt cgttttccgc tacgtaacct
    30181 cttcttctgg atgattttta tcaccctgat gctgccggtt gaagtacgta tcttcccgac
    30241 ggtggaagtc atcgccaacc tgaagatgct cgacagctac gccggtttaa cgctgccgct
    30301 gatggcctcg gcgaccgcca cgtttctgtt tcgccagttc tttatgacgc tgccggatga
    30361 actggtggaa gcggcgcgga tcgacggcgc gtcaccaatg cgtttctttt gcgacatcgt
    30421 ttttccgctc tccaaaacca atctggcggc gctgtttgtg atcaccttta tttacggctg
    30481 gaaccagtat ttgtggccgt tgttgattat taccgatgtg gatctcggca ccaccgtggc
    30541 agggatcaaa gggatgatcg ctacaggcga aggcaccact gaatggaact cagtgatggc
    30601 ggcgatgttg ttaacgctta tccctccggt ggtgattgtt ttagtgatgc agcgtgcctt
    30661 cgtgcgcggc ctggtcgata gtgagaaata agatggcagg actgaaatta caggcagtaa
    30721 ccaaaagctg ggatggtaaa acccaggtca ttaaaccgct gacccttgat gtggcggatg
    30781 gcgaatttat cgtgatggtc gggccgtctg gctgcgggaa atcgacgctg ctgcgcatgg
    30841 ttgccgggct ggagcgggtg acagaaggcg atatctggat caacgaccag cgcgtgactg
    30901 aaatggagcc aaaagatcgc gggattgcga tggtgttcca gaactacgcg ctttatccgc
    30961 atatgagtgt cgaagaaaac atggcgtggg ggctgaaaat tcgcggcatg ggcaagcagc
    31021 aaattgccga gcgcgttaaa gaagcggcgc gcattctgga gctggacggt ctgctcaaac
    31081 gtcgcccgcg cgagctttcc ggcggtcagc gccagcgtgt ggcgatgggc cgcgcgattg
    31141 tgcgcgatcc ggcggtgttc ctgtttgatg agccgctctc taacctcgat gccaagctgc
    31201 gcgtgcagat gcgtcttgaa ctgcaacagt tgcaccgtcg cctgaaaacg acttcactct
    31261 acgttactca cgatcaggtt gaagcgatga cgctcgccca gcgagtaatg gtgatgaacg
    31321 gcggcgttgc cgaacagatt ggcacaccag ttgaagtcta cgaaaagccc gccagcctgt
    31381 ttgtagcgag ttttatcggc agtccggcga tgaacctgct gacaggccgc gtgaataacg
    31441 aaggcacgca tttcgaactg gacggcggta ttgagctgcc gctaaacggt ggctaccgtc
    31501 agtatgccgg gcgtaaaatg actctcggca ttcgcccgga acatattgcg ctaagctcgc
    31561 aggcagaagg cggcgtaccg ctggtgatgg acacgctgga gatcctcggc gcagataacc
    31621 tggcgcacgg acgctggggc gagcagaagc tggtggtgcg actggcgcat caggagcgcc
    31681 cgacggcagg cagcacgctg tggctgcatc tggcggaaaa tcagctgcat ctttttgatg
    31741 gtgaaacagg acaacgagta tgagtaactg gccttatccc cgcatcgtcg ctcatcgtgg
    31801 cggcggtaag ctggccccgg aaaacaccct ggcggcaatc gacgtcgggg caaaatacgg
    31861 tcataagatg atcgaatttg acgcgaagtt atcgaaagat ggcgagatct tcctgctcca
    31921 tgacgacaat ctcgaacgca ccagcaacgg ctggggcgtc gcgggtgaac tgaactggca
    31981 ggatttactg cgcgtggatg cgggcagttg gtacagcaaa gcgtttaaag gtgagccgct
    32041 gccgttgctt tcgcaggtgg cggaacgctg tcgcgaacac gggatgatgg cgaatatcga
    32101 aatcaaaccc accactggca ctggaccatt aacgggcaaa atggtggcgc tggcggcacg
    32161 ccaactgtgg gccggtatga cgccgccgct gctgtcatcg tttgagattg atgctttaga
    32221 agctgcacag ctggcggcac cggaactgcc gcgtggttta ttgcttgatg agtggcgcga
    32281 cgactggcgc gaactgaccg cgcggctggg ctgcgtctct attcatctca atcataagtt
    32341 actcgataaa gtgcgagtga tgcagttgaa agacgccgga ctgtggattc tggtttatac
    32401 cgtcaataaa ccccagcgcg cggcagagtt gctgcgctgg ggcgtggatt gcatctgtac
    32461 cgatgcgatt gacgtgattg gtccgaactt tacggcccaa tagttttcaa cggaatgtca
    32521 ggctgcggcg tgctgggctg attcagcatg tcgccgtttc tcctctccgg cagcatatgc
    32581 tgctgattca acgaactatc cggattacgg ttgctgttta acatcccgcc gttggtgttg
    32641 ggcagcattt gctgccgggc gggatttcgc tcccccggct gtgactgcaa cacccgctga
    32701 gaattattgt ttatctggtt ttctaaatgc tgctgttgca actgagtttg cgttttcagt
    32761 tgctgattca gcatcccttt ttgctggatt tgctgagtct gcatctgggt ttgcatccgc
    32821 tgctggctgg gtatctgata ccccggctgg ttagggttgt tcagagtatt aatgggctgt
    32881 gcaaagccga caaacggcag gagtgccgta agaatcagaa gccgtttcat cgcgtatcct
    32941 cctctgaaga tatcctttaa gtttactcgc ttcccgacaa aacgatgatt aattcagagt
    33001 tatataccag gcttagctgg ggttgcccct taatctctgg agaataacga tgataaaacc
    33061 gacgttttta cgccgggtgg ccattgctgc tctgctctca ggaagttgtt ttagcgccgc
    33121 cgccgcgcct cctgcgccgc ccgtctcgta tggtgtggag gaagatgtct tccacccggt
    33181 acgcgcgaaa cagggaatgg tagcgtctgt ggacgccact gccactcagg tgggggtgga
    33241 tattctcaag gagggcggga atgccgttga tgccgccgtg gcggtgggct acgcgctggc
    33301 ggtaacgcat ccgcaggcag ggaatctggg cggtggtggt tttatgctga tccgctcgaa
    33361 aaatggcaat accaccgcta tcgatttccg cgaaatggca cccgctaaag cgacccgcga
    33421 tatgttcctt gatgaccagg gcaacccgga cagcaaaaaa tcactcactt cgcatctggc
    33481 ttccggcaca ccaggtacgg tcgctggttt ctcgctggcg ttggataagt acggcactat
    33541 gccgttgaac aaagtggtgc agcccgcgtt caaactggca cgcgatggtt ttatcgttaa
    33601 cgatgctttg gcagacgatc tcaaaaccta cggtagcgaa gtgttgccga atcacgaaaa
    33661 cagcaaagct atcttctgga aagagggcga accgctgaaa aagggcgaca cgctggtgca
    33721 ggcgaacctg gcaaagagcc tggagatgat tgctgaaaac ggcccggacg aattctataa
    33781 aggcacgatt gcggaacaga tcgcccagga gatgcagaaa aacggtggct tgatcactaa
    33841 agaagattta gcggcctata aagcggtcga acgcactccg ataagcggcg attatcgcgg
    33901 gtatcaggtt tactccatgc caccgccatc ctccggcggg atccatatcg tacaaatcct
    33961 caatattctg gaaaacttcg atatgaagaa atacggcttt ggcagcgccg atgcgatgca
    34021 aatcatggca gaagcggaga aatacgccta cgccgaccgc tcggaatatc ttggcgaccc
    34081 ggattttgtc aaagtcccgt ggcaggcact gaccaataaa gcctatgcca aatccattgc
    34141 cgatcaaatt gatatcaaca aagcgaagcc gtccagtgag attcgccctg gcaagcttgc
    34201 gccttatgag agtaatcaaa ctacccatta ctctgtagtg gataaagacg gtaacgcggt
    34261 ggcggtgacc tatacgctga acaccacctt cggtacgggt attgtcgcgg gcgagagcgg
    34321 tattctgctt aataaccaga tggatgattt ctccgccaaa ccgggcgtac cgaacgttta
    34381 cgggctggtg ggcggtgatg ccaacgccgt cgggccgaac aaacgcccgc tgtcgtcgat
    34441 gtcgccgacc attgtggtga aagacggtaa aacctggctg gttaccggta gcccaggcgg
    34501 tagccggatc atcactacag tgctgcaaat ggtggtgaat agcatcgatt atggcatgaa
    34561 cgtcgccgaa gcgaccaatg cgccgcgttt ccaccatcag tggttgccgg acgagctgcg
    34621 tgtcgaaaaa gggtttagcc cggatacgct caagctgctg gaagcaaaag gtcagaaagt
    34681 ggcgctgaaa gaggcgatgg gcagtacaca aagcattatg gttgggccgg acggtgagtt
    34741 gtacggcgca tccgacccgc gctcggtgga tgatttaacg gcggggtact aaggttagcg
    34801 gccctcttcg tgggaagagg gctattttgt cagggcaagc cgaaggtagc cttttttatt
    34861 tcgtaatcct gtagatattc ttccagcggc tttgccgttc ccagagaatg aatttcacca
    34921 ctgtctttat caataataaa aggtgcgtta ccagctaagc gcgcggcctc atctccagtt
    34981 tcgagaaatt ctcgtgcttc gaaacagaaa taccagccct ggctaaagcg tccatgtaga
    35041 gtaatgacga ccgggagatc tgcatcatca aggtaatggt tcgctttcgc gaatgcgtcg
    35101 tgataagtaa tcataattat aataaatatt attgttgagt gttatattat tattcctgtg
    35161 tgatacattg agcaaagacg cgttcatctt cgtcaaagat attattaaat gctggttttg
    35221 aaaaaagttc atcggataag atatagtcac gaaataagta tctgctaata ttttatgatt
    35281 tctttttcag aacgtcgaac gggattttct tatttaagat atcagaatct tatgattcgg
    35341 gtaaaattct aaataaatca acatgtcatt aaaatcatca tagccattag ctatttcggc
    35401 taaaatagag actacatgtc ttcggtccat ctcacttaag gagtgtagtt ccgttgtaag
    35461 tttttccata gcttgcactg ctaaatttcg aacaaggaat tttctgctgg taatctctaa
    35521 aaagatggca tggtttacaa tgatttttgt ttccttttga ttattatgaa caactgtcca
    35581 tgatttcgtt taagaatgaa gagaaatcac taaacgaact gaatatattt tctgtgccaa
    35641 tattatctct aatttcaaaa aag
//
LOCUS       DAZGOB010000041        35548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGOB010000041 DAZGOB010000000
VERSION     DAZGOB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.041"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(71..1039)
                     /gene="astE"
                     /locus_tag="ACHLIF_002117"
     CDS             complement(71..1039)
                     /gene="astE"
                     /locus_tag="ACHLIF_002117"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO9606129.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1032..2375)
                     /gene="astB"
                     /locus_tag="ACHLIF_002118"
     CDS             complement(1032..2375)
                     /gene="astB"
                     /locus_tag="ACHLIF_002118"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO9606130.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(2372..3850)
                     /gene="astD"
                     /locus_tag="ACHLIF_002119"
     CDS             complement(2372..3850)
                     /gene="astD"
                     /locus_tag="ACHLIF_002119"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO9606131.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPPPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(3847..4881)
                     /gene="astA"
                     /locus_tag="ACHLIF_002120"
     CDS             complement(3847..4881)
                     /gene="astA"
                     /locus_tag="ACHLIF_002120"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO9606132.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(4878..6098)
                     /gene="astC"
                     /locus_tag="ACHLIF_002121"
     CDS             complement(4878..6098)
                     /gene="astC"
                     /locus_tag="ACHLIF_002121"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO9606133.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQLGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            6544..7350
                     /gene="xthA"
                     /locus_tag="ACHLIF_002122"
     CDS             6544..7350
                     /gene="xthA"
                     /locus_tag="ACHLIF_002122"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO9606134.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            7517..8227
                     /locus_tag="ACHLIF_002123"
     CDS             7517..8227
                     /locus_tag="ACHLIF_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO9606135.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLLGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8232..8909
                     /gene="ydjY"
                     /locus_tag="ACHLIF_002124"
     CDS             8232..8909
                     /gene="ydjY"
                     /locus_tag="ACHLIF_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO9606136.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            8924..9631
                     /locus_tag="ACHLIF_002125"
     CDS             8924..9631
                     /locus_tag="ACHLIF_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO9606137.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            9631..10179
                     /locus_tag="ACHLIF_002126"
     CDS             9631..10179
                     /locus_tag="ACHLIF_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO9606138.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10189..11355
                     /locus_tag="ACHLIF_002127"
     CDS             10189..11355
                     /locus_tag="ACHLIF_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9606139.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            11328..12863
                     /locus_tag="ACHLIF_002128"
     CDS             11328..12863
                     /locus_tag="ACHLIF_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO9606140.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPTLLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            12863..13516
                     /locus_tag="ACHLIF_002129"
     CDS             12863..13516
                     /locus_tag="ACHLIF_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9606141.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            13583..14890
                     /gene="ynjE"
                     /locus_tag="ACHLIF_002130"
     CDS             13583..14890
                     /gene="ynjE"
                     /locus_tag="ACHLIF_002130"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO9606142.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(14899..15519)
                     /locus_tag="ACHLIF_002131"
     CDS             complement(14899..15519)
                     /locus_tag="ACHLIF_002131"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO9606143.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            15606..16013
                     /gene="nudG"
                     /locus_tag="ACHLIF_002132"
     CDS             15606..16013
                     /gene="nudG"
                     /locus_tag="ACHLIF_002132"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO9606144.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(15979..16251)
                     /locus_tag="ACHLIF_002133"
     CDS             complement(15979..16251)
                     /locus_tag="ACHLIF_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO9606145.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            16487..17830
                     /gene="gdhA"
                     /locus_tag="ACHLIF_002134"
     CDS             16487..17830
                     /gene="gdhA"
                     /locus_tag="ACHLIF_002134"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO9606146.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(17947..18987)
                     /locus_tag="ACHLIF_002135"
     CDS             complement(17947..18987)
                     /locus_tag="ACHLIF_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO9606147.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEYFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(19115..21076)
                     /gene="topB"
                     /locus_tag="ACHLIF_002136"
     CDS             complement(19115..21076)
                     /gene="topB"
                     /locus_tag="ACHLIF_002136"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO9606148.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(21081..22124)
                     /gene="selD"
                     /locus_tag="ACHLIF_002137"
     CDS             complement(21081..22124)
                     /gene="selD"
                     /locus_tag="ACHLIF_002137"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO9606149.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIH"
     gene            complement(22241..22792)
                     /locus_tag="ACHLIF_002138"
     CDS             complement(22241..22792)
                     /locus_tag="ACHLIF_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO9606150.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            22953..24809
                     /gene="sppA"
                     /locus_tag="ACHLIF_002139"
     CDS             22953..24809
                     /gene="sppA"
                     /locus_tag="ACHLIF_002139"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO9606151.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            24976..25992
                     /gene="ansA"
                     /locus_tag="ACHLIF_002140"
     CDS             24976..25992
                     /gene="ansA"
                     /locus_tag="ACHLIF_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO9606152.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            26003..26644
                     /gene="pncA"
                     /locus_tag="ACHLIF_002141"
     CDS             26003..26644
                     /gene="pncA"
                     /locus_tag="ACHLIF_002141"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO9606153.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(26805..27077)
                     /locus_tag="ACHLIF_002142"
     CDS             complement(26805..27077)
                     /locus_tag="ACHLIF_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO9606154.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(27119..27532)
                     /gene="msrB"
                     /locus_tag="ACHLIF_002143"
     CDS             complement(27119..27532)
                     /gene="msrB"
                     /locus_tag="ACHLIF_002143"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO9606155.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            27874..28869
                     /gene="gapA"
                     /locus_tag="ACHLIF_002144"
     CDS             27874..28869
                     /gene="gapA"
                     /locus_tag="ACHLIF_002144"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO9606156.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            28953..29837
                     /locus_tag="ACHLIF_002145"
     CDS             28953..29837
                     /locus_tag="ACHLIF_002145"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO9606157.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(29888..30742)
                     /gene="yeaE"
                     /locus_tag="ACHLIF_002146"
     CDS             complement(29888..30742)
                     /gene="yeaE"
                     /locus_tag="ACHLIF_002146"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO9606158.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(30832..31578)
                     /gene="mipA"
                     /locus_tag="ACHLIF_002147"
     CDS             complement(30832..31578)
                     /gene="mipA"
                     /locus_tag="ACHLIF_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO9606159.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            32014..33948
                     /gene="yeaG"
                     /locus_tag="ACHLIF_002148"
     CDS             32014..33948
                     /gene="yeaG"
                     /locus_tag="ACHLIF_002148"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO9606160.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            34061..35344
                     /locus_tag="ACHLIF_002149"
     CDS             34061..35344
                     /locus_tag="ACHLIF_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO9606161.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         8570 a         9413 c         8963 g         8602 t
ORIGIN      
        1 ccaggattaa tctgtattaa tttataagaa agcaacttaa tacccgcaga gtgatttctg
       61 cgggtaagta ttagcttatt ttttcgagca ttaatcccgc gcgtaatccc aacgctacca
      121 acggattagg gaataacaca tactctacat catgggttac ggtaaaacgt tcctctccgt
      181 cctgcgccag caatgttcct ttctcaaacg gcataaaatt cagcgtgtca cttgccatat
      241 gcatttcgaa ggacggcgag tggcgagtaa tttgcgaaac cacccgataa cggagcggcg
      301 gtgttctcac gataccgaca ctctcaccag atagcagcgc agcaattgcg ctggcagtta
      361 ctgcaaactg gcgaagatcg ttttgcccaa agggcaacgc tttgccaagt tccagcgtac
      421 aggccagcgc gccaaaatgt ctggcgctga aatgggtaaa cgtaccacca ggttcctgat
      481 ggaacaccag cgcctccagc cccgccgcac ccagccacgt cagaaatttc tcgtcccagg
      541 gaatgtcgcg ttgcggtaat acaccgaact gcggatgcaa ggagccacga attgcggtat
      601 gtagatcaag gtgccagcgc acagattctt tgccctggtc ataaaaatct tccaggcact
      661 gttccagttc gcgcgcccga caggtttctc cgctttcagc aaatagctgc caacgaccgc
      721 caaacattcg attcatatcg ctatggcaat aacgtttccc ttgcttcagc gcaggaggat
      781 tcccgaggat caccagcaac cgccaacgta acgggatctc gccgtgagat atcgcgccaa
      841 gcaacgcgtc cagcatctcc acaggtgccg tctcattacc gtgtattccc gctgaaatca
      901 ccagtgcgcc ttgcggtggc gttaatggcg tcagttccag cacaccatcg cccagccagc
      961 gccagcgaac gccgttgatt tctcgctcgg tgataaccgg ttttttaccc gttaaggtca
     1021 gagcaagaaa attatccatt gccccctccc tcgcgctgga acggataaac cgaaccgaga
     1081 ttcagtaatt ggctcaatac atccagtgct tcccgccctt cgcgcagcaa ttgcgggtcg
     1141 gccagatcgg cagcagtaag gcgatcgcgg tagtaacgat ccacccagtc attgagcgca
     1201 ttaaacagcg tatcgttcat catcaccgcc ggattcaccg cccggcgttc ttcttctgtc
     1261 aataccaccc gcaaccgcag gcacgccggg ccgccgccat tcgccatgct ttcacgtaaa
     1321 tcaaagactt ttagttcgct aatcgggttg tcagcggcaa ggagttcatt gagataaccc
     1381 catactccgg cgtgttcccg acactcctga ggcagcacca acatcataga accatcatcg
     1441 cggctcaaca gttggctgtt aaacagatag gtagacaccg catccgacac ggaaacctga
     1501 gttgccggaa cttctatcgc cataaaaccg ttcacccgcg cacgcaggtt tgccagtaac
     1561 tgtgactggc gagcgaacgc ctgttggtgg caaaacagca cctggcggtt actcacggca
     1621 atcacgtcat tatgaaaaac gccctggtcg ataacgtccg ggttttgctg ggcgaaaatc
     1681 acctgttggg gattcacctg attcagcctt gccaccgcct cgctggcttc gcgagtctgt
     1741 cgcgccggat aacgggaagg ccgggtatca ttgccttctt ctcgcccgta gacaaaaagt
     1801 tgcatacccg gctcaccgta atgaccgccg agacgattgt ggtttgccgc cccctcatca
     1861 ccgagcaacg ctacctgcgg caacgccgaa tgaacgctaa atttctcttc gtcgttaaaa
     1921 atcgctttta aaagtgattc ggtgacgtgc gcttccagcg agcggtgaaa tttattgttc
     1981 aggttggcaa cggtgagatg cactttgcca tccagcgtat cggcagatgg cgcgatcgtt
     2041 gccgcattgg ctacccacat tggcgaagcg gaactgacgc tggaaagcca gtgcggtgcc
     2101 tggcgtgcaa ctttttccag tacctgctca tcgctaccac tgaatcccaa ctgacgcagc
     2161 accggaataa acggacgctc gtgcggcggg atcacggcct gggggaatcc cgcatcggca
     2221 agggctttca ttttcagtaa gccctgtttt gccgcctgtc gcgggttaga cacctgaaaa
     2281 cggtgacggg tagaggcttc attaccaaac gacaggccag cgtaatgatg cgtcagccct
     2341 accagcccgt cgaaattaac ttcccaggcg ttcatcgcac cacctcatcg gaaaaatcca
     2401 gcccggggtt aagcgttgcg ggcaaagtta acgagtccga ctccaggctc gccataggcc
     2461 atgcgcagta atctgcggca taccaggcgc tggggcgatg gttaccggat gcaccaatgc
     2521 cgccgaatgg cgcggtactg gcagcaccag taagcggttt gttccagtta acaatccccg
     2581 cccgcgcctc cagcaacagt tgatcgaatt tttcccgctc gggggaaacc agaccgcaag
     2641 agagtccgaa gcgagtgtta ttcgccatta gaatcgcttc ctcgaaagaa tcataacgcc
     2701 agacgcgcaa taacggtccg aacacctctt catctggtac gccagcaacg cctgtcattt
     2761 caatgatccc cggcgtcagc aacgatgtct ctgattgtaa taagcgcggc gcaagcaggg
     2821 ttcgtccgcc catcgcttcc agttgctgcc aggcagtaac cacctgctgt gcggcctgtt
     2881 cagaaatcag cccgccaata aacggcggcg gttcgtcatc ccagttgccc ggcgttaatc
     2941 gctggctgac ggcaaccaga cgagcaagaa acgcatcgcc ctgcgctccg cttttgagca
     3001 ataaacggcg ggcgcaggtg cagcgttgcc cggctgtgac aaacgccgac tgaatggtca
     3061 gatggacagc cgcgtcgata tccgccacct catcgataat tagcggatta ttaccgccca
     3121 tctcaagggc gagaattttc tccggctgac cggagagctg gcgatgcagc tggtagccgg
     3181 tattggcgct accggtaaac agcaaaccgt cgagatcctc cagcgcactc agcgcctgac
     3241 ccgtttcacg cccgccctgc accaggttca gtacgcccgg cggcaagcca gcctgctgcc
     3301 ataaacgcat taccgcttcg ccactccacg gtgtcagttc gctgggttta aagataatgg
     3361 tgttacctgc cagcaatgcc ggaacgatat gtccgttcgg caaatgacca gggaaattat
     3421 acggcccaaa caccgccagc acgccgtgcg ggcgatgtcg caggctcgcc gcaccgtccg
     3481 gcatttcact acgctgctcg ccggtacgaa cgtgatacgc cttaattgat atcgcgattt
     3541 tattgatcat cgccgtcact tcggttgccg cttcccagcg cggcttaccc gtttctctgg
     3601 caataatcgc ggttaattcg cctttattcc tttccagcaa tccggcaaag cgttcgacaa
     3661 cgacctgacg ttcagccaat gagagccgcg cccagcgcgg aaacgccgca cgggctgccc
     3721 gacaagcctg ttcgacctga gcggcatcgg catcattgcc ttgccataac acctcgcccg
     3781 ataccggatt acgcttcaca cgcgatgcgc cctggcccgt tatccagtca ccgttaatcc
     3841 ataaagtcat gctgttttct cctctgcgca caggcgcacc agacgaacgc gatcccctgc
     3901 gtggcatttg agggcatcca gttgtgcggc ggttaaaatc aaacgctcgg ttgccggatc
     3961 ggtacgcgcc agcaccacgc ggaaatggtg ataattttca ttggcgacca ggcaggccgg
     4021 aaaatcgccc tgcgcaggct gcccttctgc cacttccacc agccgacttt tacgaatggc
     4081 gcgcacgcgg tcgatgtcac actcaagcgt tggcccgccg tcgaagatgt cgatatagtt
     4141 gcggtaacga aaaccttctt tctccagcac cgcgcgggca ggcgcggttt gcggatgtac
     4201 ctgaccgatg acgtcctggg cttcctggga taaaaagtgg gtatagatcg gatgtttcgg
     4261 catcagttct gcaataaatg ccttttgccc ggtgccgcag agaaaatcgg cgcggctaaa
     4321 atccatcgaa aagaagcgtt taccgaggct ttgccagaac ggtgaatagc cgtgttcgtc
     4381 aatcaccccg cgcatttcag caaccacttt gtcattaaac ttgtcgcgaa aagccgccat
     4441 aaacataaag cgcgatttcg acagcaaata gccgttgccc tctttgcgcc agtccgggtc
     4501 gagaaacagc gtgcacagct cgctgctgcc ggtgtgatcg ttactgagaa acagcgtcgg
     4561 caatgcgtta tagacattca gctcttttga ggcgtgaacc aacgtgccga cgcgatagtt
     4621 gtaccaggga tcgttcagcc caaccgccac ctcaatggca caaatccccg ccacggtgcc
     4681 tgtctcgcta tcttccagca cgaacacata gccctgctca cttttgggca gttcgccttg
     4741 ccaggttttg attgcccttt cgatacgcgc cgaaagcgtg gcttcattgg cgggaagcga
     4801 cgtcaggccg ccgcccgttt tgctggcaag ctgcatcagc gccgagacat ctgatcgctc
     4861 aacgggacgg atgaccatca tgatgaacct cggctaacaa agtgttcgca agcagctgca
     4921 aagcgatcca gtccggtcgt cacctcttct tcgctgacat tgagcgcagg cgcaaaacgc
     4981 accacgttgc cacccgcaat cagtaccatc acgcctgctt tcgccgcttc ctgagagatc
     5041 tgtttcgctt gcccggcgta atcggcattc agtacacagc caatcagcaa acctaagccg
     5101 cgaacttcac tgaacaaacc atagcggtga ttaatagtat taagacgctc aacaaaccag
     5161 tcgtgacgct gtttaacgcc attaagcatc tctggtgtgt tgatgagctc cagcactttg
     5221 cctgccaccg ccgaggccag cggattaccg ccataggtgg tgccatgagt gccaacggtc
     5281 atcacgcggg cgcactcttc ggttgccaac aacgcaccga ccgggaaacc gccgcccagc
     5341 gctttggcgg tagttaacag atcaggcgtc acgccgtagt gcatataggc atacagttcc
     5401 ccggtgcgcc cgacgccggt ttgtacttca tcaaaaatca acagcgcatt gtggcggtta
     5461 cacaattcac gcagaccttg taaaaacgcg ttgctggctg gcaccacacc gccttccccc
     5521 tggatgggtt cgacaatcac cgcacaggta gagtcgtcaa tcagcgcgct ggcagagtta
     5581 atatcgttat atgcagcatg acgaatatcc gccggcagtg gcgcaaaatc ctgtgaatag
     5641 gctggctgcc cacccgcact gacagtaaac agcgtgcgac catgaaacgc atttttgaac
     5701 gccacgatgc cgctcttatg gctgccgtag cggtcgtgag cgaatttacg cgccagtttt
     5761 agcgccgctt cgttggcttc cgcaccggag ttacaaaaga agacgcgatc ggcaaacgtg
     5821 gcgtcgatca attttttcgc cagtcgcagt accggctcgt tggtgtaacc gttgccggta
     5881 tgccagaact tactcgcctg ttcgttcagc gcttcacgca gttccggatg cgcatggccc
     5941 agcgcgttca ccgcaatgcc acccgcgaag tcgatatact ctttccccag ctgatcccac
     6001 aagcgcgaac cttcgccacg taccggtata aagggtgccg gagcgtaaac aggtatcatc
     6061 cattcatcaa agttttcacg cgtaattggc tgagacatag cgacctctac agtaaataat
     6121 tcgttattta tatgttaaca ataagtaatg tttgcgctgt aaatgtagat tgcagggttc
     6181 gtgccagcca gtgataaaag tgcataaacg gcggaggcta actggaaatc aaggagttat
     6241 aaccaaacca tatgcattta aagtgcatat aaagtgaata cgtttgcgat gtgggtgaat
     6301 aaaaagaata aaaaacgcaa tgttatgcag aagtaaaata taattctgga attgtgatca
     6361 ttgacgaaat ttactggaaa ttactgcgcc attctgacgc agcgcgcacc aaaagcgggc
     6421 attttttgcg ccatcgttga catcattaac aaccatcgat caaatcactt aacaacaggc
     6481 ggtaagcaac gcgaaattct gctaccatcc acgcactctt tatctgaata aatggcagcg
     6541 actatgaaat ttgtctcttt taatatcaac ggcctgcgcg ccagacctca ccagcttgaa
     6601 gccatcgtcg aaaagcacca accggatgtg attggcctgc aggagacaaa agttcatgac
     6661 gatatgtttc cgctcgaaga ggtggcgaag ctcggctaca acgtgtttta tcacgggcag
     6721 aaaggccatt atggcgtggc gctgctgacc aaagagacgc cgattgccgt gcgtcgcggc
     6781 tttcccggtg acgacgaaga ggcgcagcgg cggattatta tggcggaaat cccctcactg
     6841 ctgggtaatg tcaccgtgat caacggttac ttcccgcagg gtgaaagccg cgaccatccg
     6901 ataaaattcc cggcaaaagc gcagttttat cagaatctgc aaaactacct ggaaaccgaa
     6961 ctcaaacgtg ataatccggt actgattatg ggcgatatga atatcagccc tacagatctg
     7021 gatatcggca ttggcgaaga aaaccgtaag cgctggctgc gtaccggtaa atgctctttc
     7081 ctgccggaag agcgcgaatg gatggacagg ctgatgagct gggggttggt cgataccttc
     7141 cgccatgcta atccgcaaac agcagatcgt ttctcatggt ttgattaccg ctcaaaaggt
     7201 tttgacgata accgtggtct gcgcatcgac ctgctgctcg ccagccaacc gctggcagaa
     7261 tgttgcgtag aaaccggcat cgactatgaa attcgcagca tggaaaaacc gtccgatcac
     7321 gcccccgtct gggcgacctt ccgccgctaa tttagcagct ctcctggctc aaactgggtc
     7381 aggagaatta accttgagaa aaatcaacaa actgtcagta atgatttgtt gcctgccgtc
     7441 ctttgttata ccgtcactgc gtttttagtt gtctgaccac ttctctatta tcaagtttga
     7501 tataggaaac tccacgatga acgctgagcg taactttctg tttgcctgtc ttatttttgc
     7561 gctggtcatt tacgctatcc acgctttcgg tttattcgat ctgctcaccg atttacccca
     7621 cttacagaca ctcattcgcc agagcggatt gttcggttat agcctctata ttctgttatt
     7681 catcattgcc accctctttc tgttaccagg aagcatactg gtgatcgctg gaggaatagt
     7741 ttttggcccg ctcttaggga cactactctc attaattgcc gcaacgctgg cctcctcgtg
     7801 ctcattcctg ctggcgcgct ggctgggacg tgatttactg ctgaaatacg tgggccatag
     7861 ccataccttc caggccattg aaaaaggcat tgcgcgtaac ggtattgatt ttcttattct
     7921 gacccgctta atcccgttgt ttccttacaa tattcaaaat tacgcttacg gattaaccac
     7981 catcgccttc tggccttata cccttatttc ggcactcacg accctgctcg gtattgttat
     8041 ttataccgtg atggcaagcg atctcgccaa tgaaggcatt acgctgcgct ttattttaca
     8101 actctgtctg gcgggcctgg cgctgtttat tctcgtccag ctcgcaaaac tctacgcccg
     8161 acacaaacat gtggatctgt ctgcttcgcg ccgcagccca cttactcacc caaaaaatga
     8221 aggatagaac gatgtcgcaa cattattcag tgtcatggaa aaaaggactg gctgcactct
     8281 gcttactggc tgttgccggg cttagcggct gcgatcaaaa agagaatgcg gcggcaaaag
     8341 tggaatacga cggactttcg aacagccaac cgctgcgcgt cgatgccaat aaccatacgg
     8401 taaccatgct ggtgcaaatt aatggtcgtt tcctcaccga cgacactcgt cacggtattg
     8461 tgtttaaaga tggctccaac ggacataaat cgctgtttat ggcttatgcg accccgaaag
     8521 cattttatga agccctgaaa gaggcaggtg gtacgccggg cgaaaacatg acgatggata
     8581 ataaagaaac gactcatgtc acaggcagca aactggatat ttcggtcaac tggcaagggg
     8641 cggcaaaagc gtattccttc gatgaagtga ttgttgacag taatggcaag aaactggaca
     8701 tgcgctttgg cggtaattta acggcagcag aagagaagaa aacgggttgc ctggtgtgtc
     8761 tggatagctg cccggtcggc atcgtcagca atgcaacata cacttatggt gcggtggaaa
     8821 aacgcggcga agtgaaattc aaaggcaatg cctcagttct cccggcggat aacacgctgg
     8881 caacggttac ctttaaaatc accgaataaa gccaggataa aggatgatga agatacaatc
     8941 gcgtaaaatc tggtactacc gtataaccct catcatcctg ttgttcgcta tgctgctcgc
     9001 atgggcgctg cttcccggcg tccatgagtt tatcaatcgc agcgttgcgg cgtttgccgc
     9061 cgtggaccaa cagggtatag aacgctttat tcagtcttac ggcgcactgg cggcagttgt
     9121 ctcgttcttg ttgatgattt tacaggccat tgctgcaccg ctacctgcgt ttttgatcac
     9181 ctttgccaat gcgtcgctgt ttggcgcgtt ctggggcggc ttgctgtcgt ggaccagttc
     9241 gatggccggc gcggcgctgt gcttttttat cgccagagtg atgggccgcg aagtggtgga
     9301 aaaattaacc ggcaaaaccg tgcttgacag tatggacggc tttttcactc gctacggcaa
     9361 acacaccatc ctggtctgtc ggttattgcc ttttgtccct ttcgatccaa tcagctatgc
     9421 tgccggtctg acttcaatac gttttcgctc gttttttatc gccaccgggc ttggtcaatt
     9481 accggcgact attgtttatt cctgggcggg cagcatgtta acaggcggta ctttctggtt
     9541 tgtcaccgga ctgtttattc tgtttgccct gaccgtggtg atttttatgg cgaagaaaat
     9601 atggcttgag cgccagaaga ggaatgcctg atgggtttac cgccgcttag caaaattcct
     9661 ttcattttac gtccgcaggc gtggctgcat cgtcgccatt acggcgaggt gctaagcccg
     9721 attcgctggt gggggcggat cccgtttatc ttttatttgg tgtcaatgtt tgttgggtgg
     9781 ctggagcgca aacgctcacc gctcgatccg gtagtgcgat cgcttgtcag cgcgcgcatt
     9841 gcgcaaatgt gcctgtgtga gttttgcgtg gatatcacca gtatgaaagt cgccgagcgt
     9901 accggcagca ccgataaact gctggcagtg gctgactggc ggcaaagccc gctcttcagc
     9961 gatgaagaac ggctggcgct ggagtacgcc gaagccgcaa gcgtaacgcc gccaacggtc
    10021 gatgatgccc tgcgcacccg actggctgcg cattttgacg ctcaggcgct caccgaactg
    10081 acggcattga tcggcctgca aaacctgtcg gctcgtttta attctgccat ggacattccc
    10141 gctcaggggc tgtgccgtat tccggaaaaa cgttcttaag gagagaagat gcgccattgt
    10201 gggtggttgc tgggattgtt atcgctgttt tctctggcaa cacatgccag tgactggcag
    10261 gaaattaaaa atgaggccaa agggcaaacc gtctggttta acgcctgggg cggcgatacc
    10321 gcaattaacc gctatctcga ctgggttagc ggcgagatga aaacccatta cgctataaac
    10381 ctgaagattg ttcgtctggc ggatgccgca gacgcggtga agcgcattca gaccgaagct
    10441 gctgccggac gtaaaacggg cggctcggtg gatctgctct gggtgaacgg cgaaaacttc
    10501 cgcaccttaa aagaggccaa tttattacaa acgggctggg cggagactct gcccaactgg
    10561 cgctatgtcg acacacagct gccggtgcgg gaagattttt cagtgcctac agaaggggct
    10621 gaatcgccct ggggcggcgc acaactgacg tttatcgccc gccgcgatgt tacgccacag
    10681 ccaccacaaa cgccgcaagc cttactggag tttgctaaag ccaatcccgg cacggttacc
    10741 tatccgcgcc caccggactt taccggcacg gcgtttcttg aacagttgct gattatgctg
    10801 acgcccgatc ccgccgcgtt aaaagttgcc ccggataaag ccactttcgc ccgcgtcact
    10861 gcgcccttgt ggcaatatct tgacgcgcta catccctatt tatggcgcaa aggaaaggat
    10921 ttcccgcctt cgcccgcgcg gatggatgct ctgctgaaag ccggcacatt gcgcctgtcg
    10981 ctgaccttta accctgctca tgcccagcaa aaaatcgcca gcggcgattt gcctgcaagc
    11041 agttacagtt ttggttttaa ccaggggatg attggcaacg tgcatttcgt caccattcct
    11101 gctaacgcaa atgccagtgc tgcggcgaag gtggttgcca acttcctgct ctcacccaat
    11161 gcgcaactgc gtaaagcgga tcccgctgtc tggggcgatc cttctgttct cgatccgcaa
    11221 aaactgcctg atgggcagcg cgaaacattg caatcaagaa tgccgcaaga tctgccgccg
    11281 gtactggctg aaccgcacgc aggttgggta aatgcgctgg aacaagaatg gctacaccgt
    11341 tacggtacgc attaatcttt ttgctgtggg cgatggtggc ggtgatttat gcaccactga
    11401 tcccggcagc tctcacgctg atatcgcctg ctttgtcgtt gacacactgg caggcgttat
    11461 ttgccgatcc acagttaccg caggcattac tggcaacgct ggtgtcgaca accatcgcgg
    11521 cggtcggggc gttgttgatt gccctgctgg tgattgtggc gctgtggcct tggccgaaat
    11581 ggcagcgtat gtgtgcccgt ctgccgtggc tgctcgccat tccccatgtg gcttttgcca
    11641 ccagcgccct tctgctcttt gctgacggag ggctgcttta tgactatttc ccgtatttca
    11701 ctccgctaat ggacaaattg ggtattgggc tgggcctcac ccttgcagtg aaagaaagcg
    11761 catttctgct gtggatctta gcggcagtat tgagcgaaaa acggctgttg cagcaggtca
    11821 ttgtgctgga ttcgctgggc tacagccgct ggcaatgcct gaactggctg ctcttgccct
    11881 ccgtcgcacc tgcactggca atggcgatgt tggcgattgt tgcctggtcg ctttcggtcg
    11941 tggatgtggc gattattctt gggccaggta atccaccgac gctggcagta attagctggc
    12001 agtggttaac tcagggcgac gcggatcaac aaacgaaagg cgcactcgcc agtctgctgc
    12061 tgatgctgtt actcgccgcc tacgttttgc tgagctatct gctatggcgc agctggcggc
    12121 gcactattcc ccgcgtagat ggcattcgca agcctgccac acctttatta ccaggcacta
    12181 cgctggcgag ttttttaccc ttaaccggtg tgctgtgtgt ggttctgctg gcgatcctcg
    12241 cggatcagtc gacgatcaat agtgaagcac tcatcaacag cctgacaatg gggctggtgg
    12301 cgacattcat cgctttgctc ctgttactgc tgtggctgga atgggggcca cagcgtcgcc
    12361 agttgtggct ctggttgccc actctattgc ccgctctgcc actggtggcg ggccagtaca
    12421 cgctggcgct atggctgaac ctggatggaa gctggacggc ggtggtctgg gggcatctgc
    12481 tgtgggtaat gccgtggatg ctgtttatcc tgcaacccgc ctggcagcgc attgattcac
    12541 ggttaatttt gattgcgcaa acactgagct ggtcgcgggc caaaatcttc ttttacgtga
    12601 aatgtccgct tatgctacgc cctacgctga ttgccttcgc ggtgggattt tcagtcagta
    12661 ttgcgcagta tatgccgacg ctgtggctgg gcgcggggcg ttttccgacg ctcaccactg
    12721 aggcggtggc attaagcagc ggcggcagca atggtatttt cgccgcccag gctttatggc
    12781 aactgctatt accgcttatt atttttgccc tgaccgcgtt agtcgcaaaa tgggtaggtt
    12841 atgtcagaca aggactccgc taatgctctg cgtgaaaaat gtttcgctac gtttaccaga
    12901 aagccgcttg ctgacaaacg ttaactttac ggtggataaa ggtgacattg tcacgttaat
    12961 ggggccgtct ggctgtggaa aatccactct gttttcatgg atgattggtg cactggctgg
    13021 acagttttct tgtacaggtg agctatggct caatgagcaa cggattgaca tgctacccac
    13081 cgcacagcgt cagattggca ttcttttcca ggatgcactg ttatttgacc agttcagcgt
    13141 cgggcaaaat ttactgctgg cgctaccgtc gacgctcaag gggactgcca ggcgcaatgc
    13201 cgtgaaagac gcgcttgatc gagcgggact ggctgaaact tatcaccagg accctgcgac
    13261 attatcaggc ggtcagcgag cgcgggtggc cctgctgcgc gcccttctcg ctcagccaaa
    13321 agcgttactc ctggatgagc cattcagccg tcttgatgtg gctctgcgcg ataattttcg
    13381 ccagtgggtg ttcagcgaag ttcgcgaact ggcgatcccc gtcgttcagg taacgcacga
    13441 tctccaggat gttcctgctg atagttctgt tctggatatg gagcagtggt cagaaaatta
    13501 caacaaactg cgataacgca aagtttttct caatgcgtca gttcagaatg gcgcactcaa
    13561 aactacaatg tcgggatttt cgatgaaacg tgtttctcaa atgaccgcgc tggcaatggc
    13621 tttagggctg gcttgcgctt cttcgtgggc cgctgaactg gcgaagcctc ttacacttga
    13681 ccagcttcaa caacaaaatg gcaaagcgat agatactcgc cccagcgcgt tttataacgg
    13741 ctggccacaa accttaaatg gcccttctgg tcatgaacct gccgccttaa acctctctgc
    13801 cagctggctc gacaaaatga gcaccgaaca gctcaacgcg tggatcaagc aacataacct
    13861 gaaagccgat gccccggtgg cgctgtacgg taatgacaaa gatgtcgacg ccgtcaaaac
    13921 gcgactgcaa aaagcaggtt ttacgcatat ctccattctg agtgacgcgc taagcgaacc
    13981 ttcccgtcta caaaaactgc cgcactttga gcagctggtt tatccgcaat ggctgcacga
    14041 cctgcaacaa ggtaaagagg ttacggcgaa acctgccggt gactggaaag tcattgaagc
    14101 ggcctggggc gctcctaagc tttaccttat cagccatatt cccggtgctg actacatcga
    14161 taccaacgaa gtggaaagcg aaccgctgtg gaacaaagtt tctgatgaac aactaaaagc
    14221 gatgctggca aaacacggca ttcgccatga caccacggtc attctgtacg ggcgtgacgt
    14281 atacgctgga gcgcgtgtgg cgcaaattat gctctacgca ggcgtgaaag atgtgcgtct
    14341 gctggatggc ggctggcaaa cctggtccga cgcgggactg cctgttgagc gaggaacgcc
    14401 accgaaagtg aaagcggaac cggatttcgg cgtgaagatc ccggcacaac cgcagttgat
    14461 gctcgatatg gaacaggccc gtggactgct gcatcgccag gatgcatcgc tggtgagcat
    14521 tcgttcgtgg ccagaattta tcggtacgac cagcggttac agctatatta aaccaaaagg
    14581 tgaaatagcc ggagcacgtt ggggacacgc tggtagcgac tcgacgcata tggaagattt
    14641 tcataacccg gatggcacca tgcgcagcgc cgatgatatt accgctatgt ggaaagcgtg
    14701 gaatatcaaa ccagagcagc aagtttcatt ctactgcggc accggctggc gcgcgtccga
    14761 aacctttatg tacgcacgcg ccatgggttg gaagaatgtt tccgtgtatg acggcggctg
    14821 gtacgaatgg agcagcgatc caaaaaatcc ggtagcaacc ggtgaacgcg gcccggacag
    14881 tagcaaataa cattgcaatt actgacgctg gtgcgacttc agcgtcagat acccactcca
    14941 cacccgcgta aaggtcgtca tccagcacaa cgcaccgaag atccacgcaa accacgcaaa
    15001 ccatgcaggg aataaacatc ccagcacaaa cagtaagatc gtttctgtgc cttcggttaa
    15061 cccgcccaga taataaaacg atttatgcgc ataaccggga ttatcaatct gatgtttcgc
    15121 cgccagcgca gcaaaagcga gaaaactgct gcctgtgccg ataaacgcaa acagcagcca
    15181 gccgcctgcc agggcgtttt gctgcggtgc ggcgaggata aacccaaaag gtaccagcgc
    15241 gtaaaaaaga aaatcgagag aaatatcaag aaagccgccc gcgtcggtaa gccctctgcg
    15301 ccgcgccagc gcaccgtcca gaccatcaag cagcctgttc aacaaaatga cgacaagcgc
    15361 cgccagatac cagcccagcg ccagaaaagg cagtgccagc acgccgatgg caaatcccac
    15421 taatgttaaa ccatctggcg taatgcccgg tttatcaaga acccgcacgc actgatgcaa
    15481 caacggttta atccggggat gaagatggcg gtctagcaca ggcactcctt aaatataaag
    15541 cctttctgat tgagcaacag tgcggatatt atggcatttt tcgcttatct gcccgtgtgt
    15601 aatttatgaa aatgattgaa gttgttgccg ccatcattga acgtgatggc aaaattttac
    15661 tcgcgcaacg ccccgcccag agcgatcagg cgggattatg ggagtttgcc ggtggtaaag
    15721 tcgagccgga tgaaagccag cggcaggcgc tggtgcgtga gttacgcgaa gaactgggca
    15781 tcgaagcaac tgtgggtgag tatgttacca gccatcagcg agaagtttcg ggccggatta
    15841 tccatcttca tgcctggcac gtacccgact tccacgggac gttacaggca catgaacatc
    15901 aggcgctggt ctggtgctca cctgaagagg cgctgcaata tccgctggcc cctgctgaca
    15961 ttccattatt agaggcgttt atggctttac gcgccgccag accagcggat tagtgctaag
    16021 ggttttgtca tcccgctggc attgcagcag tattccctcg gctttaatta ccgcgccttc
    16081 agaataattt ttatcctgat aaacgcagca ctgagtacag ggctgcgctg actgcccgcc
    16141 tgaactgaac acttctggcg gtacgtttac ctccacgtcc ggacgataat gcgggttagc
    16201 cagtgcaatt aatggaaatg ctaatactac ggcgaacaat gctcgactca cagggaactc
    16261 cttaacgtta ttgtctctgc tactgataac ggtagccggg tggcaaaact ttagcgtctg
    16321 agttatcgca tttggttatg agattactct cgttattaat ttgctttcct gggtcatttt
    16381 tttcttgctt accgtcacat tcttgatggt atagtcgaaa actgcaaaag cacatcacat
    16441 aaacaacata agcacaatcg tattaatata taagggtttt atatctatgg atcagacata
    16501 ttctctggag tcattcctca accatgtcca aaagcgcgac ccgaatcaaa ccgagttcgc
    16561 gcaagccgtt cgtgaagtaa tgaccacact ctggcctttt cttgaacaaa atccaaaata
    16621 tcgccagatg tcattactgg agcgtctggt tgaaccggag cgcgtgatcc agtttcgcgt
    16681 ggtatgggtt gatgatcgca atcaggtaca ggtcaaccgt gcatggcgtg tgcagttcag
    16741 ctctgccatc ggcccgtaca aaggcggtat gcgcttccat ccgtcagtta acctctccat
    16801 tcttaaattc ctcggcttcg aacaaacctt caaaaatgcc ctgactaccc tgccgatggg
    16861 cggtggtaaa ggcggcagcg atttcgatcc gaaaggaaaa agcgaaggtg aagtgatgcg
    16921 tttttgccag gcgctgatga ctgaactgta tcgccacctg ggcgcggata ccgacgttcc
    16981 ggcaggtgat atcggggttg gtggtcgtga agtcggcttt atggcgggga tgatgaaaaa
    17041 gctctccaac aataccgcct gcgtcttcac cggtaagggc ctttcatttg gcggcagtct
    17101 tattcgcccg gaagctaccg gctacggtct ggtttatttc acagaagcaa tgctaaaacg
    17161 ccacggtatg ggttttgaag ggatgcgcgt ttccgtttct ggctccggca acgtcgccca
    17221 gtacgctatc gaaaaagcga tggaatttgg tgctcgtgtg atcactgcgt cagactccag
    17281 cggcactgta gttgatgaaa gcggattcac gaaagagaaa ctggcacgtc ttatcgaaat
    17341 caaagccagc cgcgatggtc gagtggcaga ttatgccaaa gaatttggtc tggtctatct
    17401 cgaacgccaa cagccgtggt ctgtaccggt tgatatcgcc ctgccttgcg ccacccagaa
    17461 cgaactggat gttgacgccg cgcatcagct tatcgccaat ggcgttaaag ccgtcgccga
    17521 aggggcaaat atgccgacca ccatcgaagc gactgaactg ttccagcagg caggcgtact
    17581 gtttgcaccg ggtaaagcgg ctaatgctgg cggtgtagca acgtcaggcc ttgaaatggc
    17641 gcaaaacgct gcgcgcctgg gctggaaagc cgagaaagtt gacgcacgtt tgcatcacat
    17701 catgctggat atccaccatg cctgtgttga gcatggtggt gaaggtgagc aaaccaacta
    17761 cgtgcagggc gcgaacattg ccggttttgt gaaggttgcc gatgcgatgc tggcgcaggg
    17821 tgtgatttaa gttgtaaatg cctgatggcg ctacgcttat caggcctaca aatgggcaca
    17881 attcattgca gttacgctct aatgtaggcc gggcaagcgc agcgcccccg gcaaaatttc
    17941 aggcgtttat gagtatttaa cggatgatgc tccccacgga acatttctta tgggccaacg
    18001 gcatttctta ctgtagtgct cccaaaactg cttgtcgtaa cgataacacg cttcaagttc
    18061 agcatccgtt aactttctgc gatagcagca gatatgccag taaagaaacc ccattagact
    18121 atttttttga taatcttctt cgctttcgaa caactcgtgc gcctttcgag aagcaagcat
    18181 tatataatgc caggccagtt cttcttcaat tgtcccgttt tgaaaagctg tgcttgatat
    18241 cgaggtcatc catgataatt ccgccgccca tattagcttc gccgaggatt taccggagcg
    18301 attattagcg caatcagaga tatagtctga gggaaaaaca gcaaatttat tcaacaaggc
    18361 gataacctgc tctggggctt cctccatgtt tgctttaaag gtattggctc catggtcgcc
    18421 agaaagaaaa tactccatta aggcacaata actttcgcta tcttcgatac cccattgatc
    18481 ctctaaagac tcgcgtcttt tacttatgat atcgatcgag tcaaagggaa gcacatgata
    18541 ttggaaggta tctttgccag gttcaggctt tcgcggccag aactccagcg tttcagacca
    18601 ttgcttatga tagaaccgat aaggtgcgat caattgtagc gcctgtaact tctcgatact
    18661 gagcggctca atacctttag cctgataata atgcagttgt tctttttttg ctttaaaacc
    18721 ggcccgaaca ataagcccca tcataattaa tagataaaga aaagagcatc ccgcggtaat
    18781 caggcctctt tcattcaaac cgttggatgt tatcgctgcg aacacaaaca ttacagcgac
    18841 aacacatgtt aaataaaacc cccacttaca aagcagcatg gccttatttt ctttaatcat
    18901 ccgttcaaaa ttactattaa atatttccca gccattaaaa gaatacttct cgctcccagg
    18961 atggttttgt aataaaactt ttttcatcac attccctgtt attacatact ttagaggcga
    19021 taattttatc catgcaaaaa aaatatccaa cgaattttct tgatccgctg gatatttcag
    19081 aatatgactc gaatagcacg aaagattcac tcgcttacgc tatcgccccg cttccgactt
    19141 catctgctgg cggacttttt ttcgcactac gtttacgcgg tgcagccttt ttcttatcag
    19201 cactgccacc actgcccgga gccacaatgc cgcgaaactg ccgcaccggc gtacgtttgg
    19261 cttgatcaat aagctgatat agcgtcccca ccagcggctg cataaagtcc tgatagcgac
    19321 actgcttttc gctgatttgc gtcagcaccg attcccagtg cgcggtcatg tccggtcgcg
    19381 tcgccatttc tggcagcgaa tggaataacg cttttccggc gtcggtagag tggatatagc
    19441 gccctttttt ggtcagaaaa ccacgcttga acagcagttc aataatcccg gcacgggttg
    19501 cttctgtccc cagaccatcg gtcgcacgaa ggatcttttt cagatctttg tcctgcacaa
    19561 agcgcgcgat cccggtcatc gccgaaagca gtgttgcatc ggtaaaatgg cgcggcggct
    19621 gggtttgccg ctctaccact tcaccttttt cacacagcaa ctcatcgcct ttcgccacca
    19681 caggcaatgg cgtaccgtcg ttttcttcat cgcgctcttt gctgcctaac agcgtgcgcc
    19741 agcctgcttc agcaagaaaa cgggctttag cgacaaattt gcctttggca atgtccagtt
    19801 cgataacaca cttgcggaac accgcatccg ggcagaactg catcaggtat tgacgggcaa
    19861 tcaggttata gaccttcgct tcgttctccg tcaggttgat cgcagaactc cgtgcggtcg
    19921 ggatgatggc gtggtgcgca tcgacctttt tgtcatccca acagcggttg cgtatatctg
    19981 gatctaccac tgactgcggc aacagatccg gtgcatgaac gctgatggca ttcatcaccg
    20041 cgtggcgtcc ggcaaaatgt tcttctggca aatagcgaca atcggaacgc ggataagtga
    20101 ttagcttgtg cgtttcgtac agcttctggc agatatcaag cacgttctgc gcgctcagac
    20161 cgaagcgttt tgccgcttca atctgtaacg ccgaaagcga aaacggcagc ggcgcggatt
    20221 ctgattcccg tttatcgtta tagctggtga caatagccgg ttgaccacta atgcggttaa
    20281 ccacatgctc cgccagtgga cgatgtaaca agcgcccttc ttcatcctgg tacggttcac
    20341 acgcttcgct cggttgccag atagcggtaa accgctcatc ggcaggtgtc acgatatgcg
    20401 ctttgacttc aaagaagtct ttcgccacga agttttcaat ctcttcatcg cggcgcacca
    20461 ccagcccgag cacgggtgtc tgcacgcgtc ccacggaaag tacgccctga taaccggcat
    20521 tgcgaccgag aatggtatac gcacgggtca tattgatgcc gtacagccag tcggcacgcg
    20581 ctcgcgccag cgcagaaacg cacagcggta caaactcact gttggaacga agacggtcga
    20641 tcgcccgctc aaccgcctgc gggttcaggt cgtttatcaa gcaacgctgc acctgctggc
    20701 gcttttccgg tgccagttgc agatagtcca gcacttcatc caccagcaat tgcccttcac
    20761 gatccgggtc cccggcgtga acgatttcgc tggcttcatg caggaagcgt ttgatgacgt
    20821 taagttgttt ggtcacggag ggtcggggct gtaattgcca cttttccggg acaatcggca
    20881 aatccgcaag attccagcgc gcatagcggc tgtcgtaggc gtctggctgc gcctgctcaa
    20941 gcaggtgacc gatacaccag gtcaccacct gaccattacc gcactcgata aagccatcgc
    21001 ctttacggtg cggtttgggc aggacatcag caatggcgcg cgccagactc ggtttttcgg
    21061 caataaacaa ccgcattgaa ttaatggatc tcaaccatgg cacgaccgcc gcgcgctggc
    21121 accagttcgc caatcgccgt cagttcaatg ccaaactcgg cggctgtagt tttaacctca
    21181 ttttctgctt ccggcatgac cgccagcagc aaaccgccag aagtttgcgg atcgcacagc
    21241 agatcgcgca cttcacgcgg catttcaccc atcagatgac cgtagctggc aaagttacgt
    21301 tcggtgccgc caggcactgc gcccaactta atgtactctt caacccccgg caattttggg
    21361 atcgcgtcat agtcaacgcg cgcctgcaca cctgcgccct gacacatttc gctcaaatgg
    21421 cccagcagac caaaaccagt aacgtcggtc atcgctttta cgccttcgat gttggcaaag
    21481 gacgcacctg cgatgttcat ccggcacatc acttccgtcg ccagtccctg atgttctggt
    21541 ttcaacagtg attttttctc agccgtggta agaacgccga tccccagcgg tttcgtcagg
    21601 aacagtttgc atccggcttg tgcggtactg tttttcttca cccgctcggt cggtacgatc
    21661 cccgttaccg ccagaccaaa aatcggctcc ggcgcatcga tggaatgacc gccagccagc
    21721 gcaatacccg cctgacggca tgcatagcgt ccaccttcgg ttacttcgcg ggcaatttct
    21781 ggggaaagtt tgttaatcgg ccagccgagg atcgcaatcg ccataatcgg tttgcccccc
    21841 atcgcgaaga tatcgctgat ggcgttagtc gccgcaatgc ggccaaaatc gaaaggatta
    21901 tcaacgatcg gcataaagaa gtcggtggta ctgataacgc tggtgccatt gcccagatcg
    21961 tacaccgccg catcgtcgcg ggtctcatta cccacaagca aattcggatc aacaaacttc
    22021 gcctgctcac tgtgcaggat ggtttccaac acttttgggg aaattttaca gccgcaacca
    22081 gctccgtggc tgtattgggt caaacgaatc gagttctcgc tcatggacat ctcctgtcaa
    22141 tgcaatccgg gtatggtaac cctcattccg tgaagtgata agtgagagtg tctgaattcc
    22201 tgcgcctttg ctcacaatcc agacagtttc gcgacaatta tcagaaataa gttacaaacg
    22261 gcgtcgggtc cgggacgtta atcgacgtag atgctttcaa ctgcggcgtg ccgaggtaga
    22321 gaaaaccgac aattttatcc tgctcacggc aaccgaatgc ttcacgcact accggacttt
    22381 cagttaatgc gccactgcgc cagatgccgc caaatccttg ggcaacggct gccatttgca
    22441 tcgccatgac cgcgcatccg gcagacattt cctgttccca tcgcgggact ttatgatttt
    22501 cttcgcattt cgccaccacc gtgatgatga gcggtgcgcg gaacggcgca ttacgggctt
    22561 tgtcgatagc tttgtcatca ctaccggcag caatcgcccc ctgttccagt acggcgctga
    22621 aacgctcgcg cccttcccct tcaatcacaa aaaaatgcca cggttgcatg gacttatggt
    22681 ccggcgcacg catacccgca cgcaggatgt tttgcagttg ttcacccgtt ggcgcgggtt
    22741 cagccaggcg ggaggcgcta cggcgattga tcaatagttc gagtgcatcc atttgattaa
    22801 ctcctgtcgt gatatttatt cacaaaatta acacgagagt ggattttgtt acagcacagt
    22861 ccgcaattcc tgctgacaag tgccggttgg gtcattacga taaccacatc tattgcgcct
    22921 gtgacaggtg tgaccttaag ttgggagaat acatgcgaac cctttggcga tttattgccg
    22981 gattttttaa atggacgtgg cgtctgctga atttcgtccg tgaaatggta cttaacctgt
    23041 tctttatttt cctcgtactg gttggtgtgg ggatctggat gcaggtcagt ggtggtgatt
    23101 cgaaagaaac ggccagtcgt ggcgcactgc tgctggacat ttctggtgtg atcgtcgata
    23161 aacccgacag ttctcagcgg tttagtaaat taagccgcca gctgcttggt gccagttccg
    23221 atcgtctgca ggaaaactca ctgtttgata tcgtcaacac cattcgccag gcgaaggacg
    23281 accgcaatat caccggtatc gtgatggatc tgaaaaactt cgcaggcggc gaccagccgt
    23341 ctatgcagta catcggcaaa gccctgaaag agtttcgtga cagcgggaaa ccggtttatg
    23401 ccgttggcga gaactacagc caggggcaat attatctcgc cagtttcgcc aataaaattt
    23461 ggctgtcacc gcaaggcgtg gttgatctgc acggttttgc taccaacggt ctgtactaca
    23521 aatcgttgct ggataagctg aaagtttcca cccatgtgtt ccgcgtgggt acgtataaat
    23581 ctgccgttga accgtttatt cgtgatgata tgtcaccggc agcccgcgaa gctgacagcc
    23641 gctggattgg tgagctgtgg caaaactatc tgaatactgt tgccgctaac cggcagatcc
    23701 ctgctcagca ggtattccct ggcgcgcaag ggttgcttga gggtttaacc aaaaccggtg
    23761 gcgataccgc gaaatatgca ctggaaaaca agctggtcga tgcactggca tcgagtgccg
    23821 aaatcgaaaa agctctgacc aaagagttcg gctggagtaa gactgataaa aattatcgcg
    23881 ccatcagtta ttacgattac gcattgaaaa cgccggcaga taccggtgac agcatcggtg
    23941 tcgtcttcgc taatggcgca attatggatg gcgaggaaac tcaggggaat gttggcggtg
    24001 ataccactgc ggcacaaatc cgcgacgctc gccttgaccc gaaagtgaaa gcgattgtcc
    24061 tgcgtgttaa tagcccaggc ggcagcgtta ccgcgtctga agtgattcgc gctgaactgg
    24121 cagcagcccg ggcagcgggt aagcctgtgg ttgtatcgat gggcggcatg gcggcatctg
    24181 gtggttactg gatttccacg ccagctaatt acattgtggc taaccccagc accctgaccg
    24241 gttctatcgg tatcttcggc gtgatcacca ccgtagaaaa tagtctggat tcgattggtg
    24301 ttcatactga tggtgtctca acttcaccgc tggcggatgt ttctatcacc agggcactgc
    24361 cgccggaagc gcagcagatg atgcaattaa gcattgagaa tggctataaa cgctttatca
    24421 cgctggttgc tgatgcgcgt cattcgacgc cggagcaaat tgataaaatc gcccagggcc
    24481 acgtctggac cggtcaggat gcaaaagcta acgggctggt cgatagtctc ggggatttcg
    24541 atgatgcggt tgccaaagca gcagagctgg caaaagtgaa acagtggcat ctggaatact
    24601 acgttgatga accgaccttc ttcgacaaag tgatggacaa catgtctggt tctgtccggg
    24661 caatgttgcc agatgcgttc caggccatgt tacctgcacc gcttgcctcg gtagcctcta
    24721 ccgttaaaag tgaaagcgac aagctggccg cgtttaacga cccacaaaac cgttatgcgt
    24781 tttgcctgac ctgcgccaac gtgcgttaag tcttgtactg agtggccgac agatcgtcgg
    24841 ccacattatt ttttacctcg acgaatcctc ttcccgctgt ctcgacccat atttccttat
    24901 ccgcatagta tcaggtgcgc tcctcctgcc tcacgtatat acttttgctc tttcgatatc
    24961 attcatatca atatcatgca aaagaaatca atttacgtcg cctacacggg cgggaccatc
    25021 gggatgcagc gttccgagca gggttatatt ccggtgtcag gtcatctaca acgccaactg
    25081 gcgctgatgc cggaattcca tcgcccggag atgccagatt tcaccattca tgaatatacg
    25141 ccgttgatgg attcttctga tatgacgccg gaagactggc agcatattgc tgaagatatt
    25201 aaagcgcact atgacgacta tgatggtttt gtcattctac acggtaccga cacgatggcg
    25261 tataccgcct ctgcgctgtc gttcatgctc gagaatctcg gtaaaccggt cattgtgaca
    25321 gggtcacaaa tcccgctggc tgagttacgc tctgacggac aaattaatct gctgaatgcg
    25381 ttgtacgttg cggcgaatta tccgatcaac gaagtaacgc tctttttcaa taaccgattg
    25441 tatcgcggca accgcactac caaagcccat gccgatggtt ttgatgcgtt tgcctctcca
    25501 aaccttcctc cgttactgga agctggtatc catattcgtc gtttgaatac gccacccgcc
    25561 ccgcacggtg aaggggaatt aatcgttcat ccaatcacgc cacaaccaat tggcgtagtg
    25621 acgatttatc cagggatttc tgctgacgtc gtgcgcaatt ttctgcgcca accggtgaaa
    25681 gcattgattc tgcgctccta tggcgtgggt aatgcgccac aaaacaaagc cttcctgcag
    25741 gaattacaag aagccagcga tcgcggtatt gtggtggtca acctgacaca atgtatgtcc
    25801 ggtaaagtga atatgggtgg ttatgccacc ggtaacgccc tcgcccatgc cggcgttatt
    25861 ggcggtgcag atatgactgt agaagccacg ctaaccaaac tgcattacct gctgagccag
    25921 gaactggata ctgaaaccat tcgcaaggcc atgagccaaa acctgcgcgg cgaactgacg
    25981 ccggatgatt aaggagactg caatgcccca tcgcgccctg ttactggtcg atttacaaaa
    26041 tgatttctgt gctggtggcg cgctcgccgt gccggaaggt gacagtacgg tagatgtcgc
    26101 taaccgcctg attgactggt gccagtcgcg cggtgaagcg gttatcgcca gtcaggactg
    26161 gcacccggcg aatcacggca gttttgccag tcagcacggt gtagagcctt atacgccagg
    26221 ccaactcgac ggtttgccac aaaccttctg gccagatcac tgtgtgcaga acagtgaagg
    26281 cgcacaatta catccgttac tgaaccaaaa agcgatcgca gcggtgttcc ataaaggcga
    26341 aaatccttta gttgacagtt acagtgcctt ttttgataac ggccgtcggc agaaaacctc
    26401 tctcgatgac tggttacgcg atcatgaaat cgatgaattg atcgttatgg gcctggctac
    26461 tgactattgc gtgaagttta ccgtgctgga cgcgttacag ttaggttata aggtaaacgt
    26521 gattaccgat ggttgtcgtg gcgtgaatat ccagccccag gacagtgcgc acgcgtttat
    26581 ggagatgtca gcagctgggg caacgctata tacgctggca gactgggaag agacgcaggg
    26641 gtaatattag ttgaattcat ctctggccta catttcctca atgacattgt aggccagata
    26701 atacttgtca gtgttgcatt taacctactt gcagctaaaa cacgacgtcc taacaagtat
    26761 atttgccctc ccttaataac aggcttaaat actagataac tcattcactt aaacatagca
    26821 ataggctttg ctgagatacc aaaatcttct tttaactgct gtttgctctt catgaccatc
    26881 tgaccatttg tgtcaattgt catatgctgt gcttcggtat tatggcgggc ttgccacagc
    26941 atcaccagtt gcaggcaatt ctctttttgt tcctcggtca acgcaacgcc atcaggccat
    27001 ttccccagtt caacggcggt cgacaaacgc tggtatacct caggcgtcat gctgttgata
    27061 atgtcatcaa gattcataat ttttccgctc ctgtggaata agttgctgaa tcgttttttc
    27121 aaccgttgat ttcttcgccg ttttcgccat cagtgaagcg taaagaagca gagttaacac
    27181 aataacgttc gcctgttggt tgcggcccgt cgggaaagac atgccccaga tgggcatcac
    27241 agttaccgca acgaatttct atgcgctgca ttccatgtga caagtctttg atataacgaa
    27301 tggattcttc actcaccggt tcgtagaaac tgggccagcc acagccggaa tcatacttgg
    27361 tttcggaatg aaacagcggg gcatcgcaga tcaaacagtg atatacgccg tcacgcttgt
    27421 tatgcagtaa acgacccgta aatggcggtt ctgtcccatg attctgcgtc acgtaaaact
    27481 gcatctcgga caaatttttt ttcagttctt ctgccgaagg tttattagcc atttgctcac
    27541 atctcacttt aatcgtgctc acattacgtg actgattcta acaaaacatt aacaccaact
    27601 ggcaaaattt tgtcctaaac ttgatctcga cgaaatggct gcacctaaat cgtgatgaaa
    27661 atcacatttt tatcgtaatt gccctttaaa attcggggcg ccgaccccat gtggtctcaa
    27721 gcccaaagga agagtgaggc gagtcagtcg cgtaatgctt aggcacagga ttgatttgtc
    27781 gcaatgattg acacgattcc gcttgacgct gcgtaaggtt tttgtaattt tacaggcaac
    27841 cttttattca ctaacaaata gctggtggaa tatatgacta tcaaagtagg tatcaacggt
    27901 tttggccgta tcggtcgcat tgttttccgt gctgctcaga aacgttctga catcgagatc
    27961 gttgcaatca acgacctgtt agacgctgat tacatggcat acatgctgaa atatgactcc
    28021 actcacggcc gtttcgacgg taccgttgaa gtgaaagacg gtcatctgat cgttaacggt
    28081 aaaaaaatcc gtgttaccgc tgaacgtgat ccggctaacc tgaaatggga cgaagttggt
    28141 gttgacgttg tcgctgaagc aactggtctg ttcctgactg acgaaactgc tcgtaaacac
    28201 atcaccgctg gtgcgaagaa agtggttatg actggtccgt ctaaagacaa cactccgatg
    28261 ttcgttaaag gcgctaactt cgacaaatat gctggccagg acatcgtttc caacgcttcc
    28321 tgcaccacca actgcctggc tccgctggct aaagttatca acgataactt cggcatcatc
    28381 gaaggtctga tgaccaccgt tcacgctact accgctactc agaaaaccgt tgatggcccg
    28441 tctcacaaag actggcgcgg cggccgcggc gcttcccaga acatcatccc gtcctctacc
    28501 ggtgctgcta aagctgtagg taaagtactg ccagaactga atggcaaact gactggtatg
    28561 gcgttccgcg ttccgacccc gaacgtatcc gtagttgacc tgaccgttcg tctggaaaaa
    28621 gctgcaactt acgagcagat caaagctgcc gttaaagctg ctgctgaagg cgaaatgaaa
    28681 ggcgttctgg gctacaccga agatgacgta gtatctaccg atttcaacgg cgaagtttgc
    28741 acttccgtgt tcgatgctaa agctggtatc gctctgaacg acaacttcgt gaaactggta
    28801 tcttggtacg acaacgaaac cggttactcc aacaaagttc tggacctgat cgctcacatc
    28861 tccaaataag ttgagatgac actgtgatct aaaaagagcg acttcggtcg ctcttttttt
    28921 tacctgataa aatgaagtta aaggactgcg tcatgattaa gaaaattttt gcccttccgg
    28981 tcatcgaaca aatctcccct gtcctctccc gtcgtaaact ggatgaactg gacctcattg
    29041 tggtcgatca tccccaggta aaagcctctt ttgcattaca gggcgcacac cttctctcgt
    29101 ggaaacctgc gggtgaagaa gaagttctgt ggttgagcaa caacacaccg ttcaaaaatg
    29161 gcgtcgctat tcgcggtggc gtaccggttt gctggccgtg gtttggtccg gcggcacaac
    29221 aaggtctgcc tgcgcacggt tttgcccgca acctgccgtg gacgctgaaa tcacatcgtg
    29281 aagatgctga tggcgtagcg ctgacttttg aattgacgca aagcgaagag acgaaaaaat
    29341 tctggccgca cgactttacg ctgttagcgc atttccgcgt gggtaaaacc tgtgaaatcg
    29401 atcttgaatc acatggcgaa tttgaaacca cctctgccct gcatacctac tttaacgtgg
    29461 gtgatatcgc taaggtaagc gtcagtgggc tgggcgatcg cttcattgat aaagtaaatg
    29521 acgcgaaaga agatgtactg accgacggta ttcagacctt ccctgaccgt accgatcgcg
    29581 tgtatctgaa tccgcaagat tgcagcgtga ttaatgatga agcgctgaat cgtattatcg
    29641 ccgtaggcca ccagcatcat ctgaacgttg tcggctggaa cccgggaccg gcgctttcag
    29701 ttagcatggg cgacatgccg gatgatggct acaaaacatt tgtttgtgta gaaacggctt
    29761 acgcttcaga aacgcaaaaa gtgaccaaag agaaacctgc acatctggcg caatccattc
    29821 gcgttgcgaa acgttaattt gcgttaatgt tgtgtgccgg gtgcgatgca tccggcacgt
    29881 atcaacatca caccatatcc agcgcagttt ttccttttgg tgccggatac gctttatcca
    29941 gcatggttaa ttccgctgac gaaagttcga cctcaagcac agccgcattc tgttggacat
    30001 gggcaatcgt ggccgctttt ggaatcgcca tcacaccctg atggctgatt acccacgcca
    30061 acaatacttg tgccgcgctg atattgtgag catgtgcaat ttcgttgact accgcgtttt
    30121 ttaacagtcc attgcgcaac cgcccggcct gggctaatgg actgtaagcc atcaccggca
    30181 tctgctgttg ctggcaccag gggagtagat cgtactcaat ccctcgtgaa ccgagatggt
    30241 aaagcacctg attagtggca cactgatttc cccccggcag ctgccagagt tcctgcatat
    30301 cagcatgatc aaggttagaa acgccccagc ggcggatttt tccctgggcg atcaattttt
    30361 ccatcgctgc cacagtctct tcaaaagcga aactgccaga ccagtgtaat aagtagagat
    30421 cgagataatc agtattgaga cggcgtaaac tggcttcgca tgcatttatc gctttttgcc
    30481 cgccagcatt ccacggatag actttagaga cgagaaagac gttatcgcgc agaccggtta
    30541 atgcttcccc aaccaccttt tcggcaccgc catcggcata catttcggcg gtatcaatga
    30601 gggttaaacc gagctcaatg cccgcgcgta gtgcagcaac ttctgttttg cgctgactgg
    30661 catcttcgcc catataccat gttccctgcc ctatggctgg cagtgagaca tcgccactaa
    30721 attgaatcat tttttgttgc attgtttcct cccaggtatt gcaccaccgt aatgcaaaac
    30781 agggcgtgac gccctgtttt tatgcacaaa atgccctgga aagatgcatt atcagaattt
    30841 gtaggtgatc ccggtagaaa tcaggccagt ccaggattta tccaccatcg ggctgtcagt
    30901 aacttcatca gacagacggg tatagcgcgc ggtaccgtaa acactccagt cgccgaggaa
    30961 gttgtagctg gcgctcagct ccaggtaagg gctccagctg tcgttcgggt tatagccacg
    31021 cagaccgctg cgagcggact ctttgcgcga tacgccataa tagtattcgt tctggttttc
    31081 gctgttccac tgcacaccga tacccggggt cacggtcagg ccgccgttgg tgtaacgata
    31141 caaccaggcc atatcccaga cgatgccgtt gctgttatcc agggtatcgc cagccagggt
    31201 ggtacgcagg taaccgtact gggtaaagtg agcataagac agaccagcca tcatggtgct
    31261 cttacggtca tccaggtgac gcatttggtg atcgccactg tctttcgctt tgaagtaaag
    31321 cggcgaccaa taagcggtaa ttgaaagttt gtccgttgcg tcattccaca ggtagtaacc
    31381 accacctaag ccacggaacc agaagttatc gccttcatag ttgattaccg gtactgggta
    31441 aacatcggta tcgtaatctt tatatgggtg ctcaacgaca cctacgcctg cgcccaggga
    31501 aaatttacct tcagcgtgcg ctacgcctgc agacgttgcg ataagcactc caagtgccag
    31561 aagtttgagt ttggtcacaa ttaatcattc cttaaacaaa tgtttagcgg gcgacaaagt
    31621 ttacccgtca atacatcgaa acccaacctt tttacgtttt cattttttaa agtaactgtt
    31681 taattttcct gacgcggatg acaccgcgct tacagccaaa tgaatttagc gttactggct
    31741 accctggtct ttacattaat tatgcaaaat ttatggatga gttgttgata tgccattgaa
    31801 attaagaaag ccgtgcaggc aagttttcca tttgccatct acgcttaatt ttgaaggtgt
    31861 atcaccgggc acgttgttct catcgtcgat aaaatggcat gagagttgct gtgttttagc
    31921 aagagacgtc gttcagttta cctcttccgg gagcctctac tattcatatg aacggctctt
    31981 aacctgtgct aaaaaacgaa aggacggcat accatgaata tattcgatca ctatcgccag
    32041 cgatatgaag ctgccaagga cgaagagttc acactgcagg agtttcttac cacttgtcgg
    32101 caagatcgca gtgcttatgc caacgcggct gagcggctat tgatggctat cggtgagcct
    32161 gtcatggtcg atacagccca ggaacccaga ctttctcgac tcttttctaa ccgggtcatt
    32221 gcacgttatc cggcatttga agagttttac ggcatggaag acgcgattga acagattgtc
    32281 tcttatctga aacacgcggc tcaggggctg gaagagaaga aacaaatcct gtatctgctg
    32341 gggcctgtgg gtgggggtaa atcatcgctt gctgagcgac tgaaatcatt aatgcagctc
    32401 gtaccgattt atgtattgag cgccaacggt gagcgtagcc cggtcaacga tcatccgttc
    32461 tgtcttttca atccgcagga agatgctcag attctggaaa aagagtatgg cattcctcgc
    32521 cgttatctcg gcaccatcat gtcgccatgg gcggcaaaac gcctgcatga atttggtggc
    32581 gatatcacta agttccgggt agtgaaggtc tggccgtcaa ttctgcaaca aattgctatc
    32641 gccaaaacgg aacccggtga tgagaacaac caggacatct ccgcgctggt tgggaaagtc
    32701 gatattcgta aactcgaaca ctacgcgcag aatgacccgg acgcctacgg ctattccggt
    32761 gcgctgtgcc gcgccaacca agggatcatg gaatttgtcg agatgtttaa agcaccgata
    32821 aaagtgctgc atccgctgtt aaccgccact caggaaggta actacaacgg gacggaaggt
    32881 atctccgccc tgccgttcaa cgggattatt ctcgcccact cgaacgaatc cgaatgggtc
    32941 actttccgta ataacaaaaa caacgaagcc ttcctcgacc gtgtttacat cgtgaaggtg
    33001 ccgtattgct tacgcatttc cgaagagatc aaaatctacg agaaattgct taatcacagt
    33061 gaattgactc acgccccctg cgcccctggc acgctggaaa cgctgtcacg tttttccatt
    33121 ctttcgcgcc ttaaagagcc agaaaactcc agcatttatt caaagatgcg ggtttatgat
    33181 ggcgaaagtc tgaaagacac cgatcccaaa gccaagtcgt atcaggaata tcgtgactac
    33241 gccggtgtcg atgaagggat gaacggtctg tcgacgcgtt ttgcgtttaa gatcctctcc
    33301 cgcgtgttca acttcgatca tgtagaagtg gcggcaaacc cggtccatct gttctacgtc
    33361 ctggaacagc agatcgagcg cgagcagttc ccacaagagc aggcagaacg ctatctggag
    33421 ttcctgaaag gttatctgat cccgaaatat gccgagttta tcggcaaaga gatccagacg
    33481 gcctaccttg aatcctattc cgaatatggg caaaacattt tcgaccgtta tgttacctac
    33541 gcggatttct ggattcagga tcaggagtat cgcgatccgg ataccgggca gctgtttgac
    33601 cgcgagtctc ttaacgccga gctggagaaa atcgagaaac cggcggggat cagtaatcca
    33661 aaagatttcc gcaacgagat tgttaacttc gtactgcgcg ccagagcgaa taacagcgga
    33721 cgcaatccga actggaccag ctatgaaaaa ctgcgcacgg ttatcgagaa gaaaatgttc
    33781 tccaataccg aggagctgtt gccggttatc tcgtttaacg ccaaaacgtc aaccgacgag
    33841 cagaagaaac acgacgactt tgtcgaccgt atgatggaaa aaggctacac ccgtaaacag
    33901 gtgcgtttac tgtgcgaatg gtatttgcgc gtacgtaaat cgtcttaaca accctggccc
    33961 ggtacacatg ttaccgggcc tacaacgaca gcgaaccgtg ggcctgagaa gcggcaacac
    34021 aggcgtagca tacagttggc aaatgtagta cggggggcat atgacctggt ttattgaccg
    34081 gcgtctgaac ggcaaaaaca aaagcatggt gaatcgccag cgttttttac gccgttataa
    34141 agcgcaaatt aaacagtcga tctccgaggc cattaataag cgttcggtga ctgacgtcga
    34201 cagcggcgaa tccgtatcca ttcccacgga agatattagc gaaccgatgt ttcatcaggg
    34261 gcgtggcggt ctgcgccacc gcgtgcatcc gggcaatgac catttcgtcc agaacgaccg
    34321 aattgaacgt ccccagggtg gcggcggagg ttccggtagt ggtcagggcc aggccagcca
    34381 ggatggtgaa ggtcaggatg aatttgtctt tcagatttcg aaagatgagt atcttgattt
    34441 gctctttgaa gatttggcct taccgaatct gaaacaaaac caacaacgcc agctgaccga
    34501 atataaaacg catcgggcgg gttataccgc taacggcgtt ccggcaaata tcagcgttgt
    34561 gcgctccttg cagaactcac tggcgcgacg cacagccatg acggcaggca agcggcggga
    34621 acttcatgca ctggaagaga atttggccat catcagcaac agtgaacctg cgcagctgct
    34681 ggaagaggaa cgtctgcgca aagaaattgc agaattacgt gccaaaattg aacgcgtccc
    34741 ttttattgac accttcgatt tacgttacaa gaactacgag aagcggcccg atccctccag
    34801 ccaggcagtg atgttttgcc tgatggacgt ttccggttca atggatcaat ccactaaaga
    34861 tatggctaag cgtttttata ttctgctgta tctgttcctc agcagaacgt ataagaacgt
    34921 ggaagtcgta tacatccgcc atcataccca ggcgaaagaa gtcgatgaac atgagttttt
    34981 ctactcgcag gaaacaggcg gcaccattgt ttccagcgcc ctgaaactga tggatgaggt
    35041 agtgaaagag cgttataacc cggcacagtg gaatatttac gctgcacaag catcggacgg
    35101 cgataactgg gccgatgact ctccgctttg ccatgaaatc ctggcgaaaa aattattacc
    35161 tgttgttcgt tattacagct atatcgaaat tacccgtcgt gcacatcaga cattgtggcg
    35221 agaatatgag catctgcaat ctactttcga caactttgcg atgcagcaca tccgcgacca
    35281 ggatgatatt tatccggtgt tccgtgaact gtttcataaa caaaatgcaa cagctaaaga
    35341 ctaaaactat cagccaggtc attatcgcct ggctgatttt tagcttactg taaattatct
    35401 catttattac atacattagc ttacaatcgc tttaaatatg acagcgtaac ctttacataa
    35461 tttagttcca gaaaacaatc attcggaaaa atgattcagt caacacgtat ttccatgggg
    35521 ttattcttta aatattgttt atcgttaa
//
LOCUS       DAZGOB010000042        35423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGOB010000042 DAZGOB010000000
VERSION     DAZGOB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.042"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..536)
                     /locus_tag="ACHLIF_002150"
     CDS             complement(<1..536)
                     /locus_tag="ACHLIF_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO9606162.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQ"
     gene            complement(796..2049)
                     /gene="amiC"
                     /locus_tag="ACHLIF_002151"
     CDS             complement(796..2049)
                     /gene="amiC"
                     /locus_tag="ACHLIF_002151"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO9606163.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2281..3612
                     /gene="argA"
                     /locus_tag="ACHLIF_002152"
     CDS             2281..3612
                     /gene="argA"
                     /locus_tag="ACHLIF_002152"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO9606164.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3674..5500)
                     /gene="recD"
                     /locus_tag="ACHLIF_002153"
     CDS             complement(3674..5500)
                     /gene="recD"
                     /locus_tag="ACHLIF_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO9606165.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5500..9042)
                     /gene="recB"
                     /locus_tag="ACHLIF_002154"
     CDS             complement(5500..9042)
                     /gene="recB"
                     /locus_tag="ACHLIF_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO9606166.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEELELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(9035..11923)
                     /gene="ptrA"
                     /locus_tag="ACHLIF_002155"
     CDS             complement(9035..11923)
                     /gene="ptrA"
                     /locus_tag="ACHLIF_002155"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO9606167.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQHLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12099..15467)
                     /gene="recC"
                     /locus_tag="ACHLIF_002156"
     CDS             complement(12099..15467)
                     /gene="recC"
                     /locus_tag="ACHLIF_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO9606168.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLADDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSVEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPDDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15480..15803)
                     /locus_tag="ACHLIF_002157"
     CDS             complement(15480..15803)
                     /locus_tag="ACHLIF_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO9606169.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15788..16195)
                     /locus_tag="ACHLIF_002158"
     CDS             complement(15788..16195)
                     /locus_tag="ACHLIF_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO9606170.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16192..16755)
                     /locus_tag="ACHLIF_002159"
     CDS             complement(16192..16755)
                     /locus_tag="ACHLIF_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO9606171.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16746..17216)
                     /locus_tag="ACHLIF_002160"
     CDS             complement(16746..17216)
                     /locus_tag="ACHLIF_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO9606172.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17400..18194)
                     /gene="thyA"
                     /locus_tag="ACHLIF_002161"
     CDS             complement(17400..18194)
                     /gene="thyA"
                     /locus_tag="ACHLIF_002161"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO9606173.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18201..19076)
                     /gene="lgt"
                     /locus_tag="ACHLIF_002162"
     CDS             complement(18201..19076)
                     /gene="lgt"
                     /locus_tag="ACHLIF_002162"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO9606174.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19227..21473)
                     /gene="ptsP"
                     /locus_tag="ACHLIF_002163"
     CDS             complement(19227..21473)
                     /gene="ptsP"
                     /locus_tag="ACHLIF_002163"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO9606175.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21486..22016)
                     /gene="rppH"
                     /locus_tag="ACHLIF_002164"
     CDS             complement(21486..22016)
                     /gene="rppH"
                     /locus_tag="ACHLIF_002164"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO9606176.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22369..22515)
                     /gene="ygdT"
                     /locus_tag="ACHLIF_002165"
     CDS             complement(22369..22515)
                     /gene="ygdT"
                     /locus_tag="ACHLIF_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO9606177.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22701..23390
                     /gene="mutH"
                     /locus_tag="ACHLIF_002166"
     CDS             22701..23390
                     /gene="mutH"
                     /locus_tag="ACHLIF_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO9606178.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23459..24172
                     /locus_tag="ACHLIF_002167"
     CDS             23459..24172
                     /locus_tag="ACHLIF_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO9606179.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24310..24528
                     /gene="ygdR"
                     /locus_tag="ACHLIF_002168"
     CDS             24310..24528
                     /gene="ygdR"
                     /locus_tag="ACHLIF_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO9606180.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24636..25676
                     /locus_tag="ACHLIF_002169"
     CDS             24636..25676
                     /locus_tag="ACHLIF_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO9606181.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25708..26901)
                     /gene="lplT"
                     /locus_tag="ACHLIF_002170"
     CDS             complement(25708..26901)
                     /gene="lplT"
                     /locus_tag="ACHLIF_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO9606182.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26894..29053)
                     /gene="aas"
                     /locus_tag="ACHLIF_002171"
     CDS             complement(26894..29053)
                     /gene="aas"
                     /locus_tag="ACHLIF_002171"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO9606183.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29639..30670
                     /gene="galR"
                     /locus_tag="ACHLIF_002172"
     CDS             29639..30670
                     /gene="galR"
                     /locus_tag="ACHLIF_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO9606184.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30677..31939)
                     /gene="lysA"
                     /locus_tag="ACHLIF_002173"
     CDS             complement(30677..31939)
                     /gene="lysA"
                     /locus_tag="ACHLIF_002173"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO9606185.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32061..32996
                     /gene="lysR"
                     /locus_tag="ACHLIF_002174"
     CDS             32061..32996
                     /gene="lysR"
                     /locus_tag="ACHLIF_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO9606186.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32983..33675)
                     /gene="ygeA"
                     /locus_tag="ACHLIF_002175"
     CDS             complement(32983..33675)
                     /gene="ygeA"
                     /locus_tag="ACHLIF_002175"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO9606187.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33804..35222)
                     /gene="araE"
                     /locus_tag="ACHLIF_002176"
     CDS             complement(33804..35222)
                     /gene="araE"
                     /locus_tag="ACHLIF_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO9606188.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8735 a         9673 c         8689 g         8326 t
ORIGIN      
        1 ttttgagaca gctcaataat ccttactgat tcataatcaa ctgaattttc ataattattt
       61 aaaatatgca atgctataaa accaatctca tcatccggta gttgaacgct aaattgctca
      121 ttaatgattg ttaatgcatc acgacttatt gcatactctg ctttgtacaa cgctttgatt
      181 tcctcaagga aaacattatt aatttgtatg cctgagacga ctcgggatat tgcattcgaa
      241 atgtgatcag caagaatcag tagtagcgat ggattgagtt ttttttcgag attactctcc
      301 gcataatgca ctatcttttc ggcaacaaaa atatactcaa tacccacatg ctcaatcaat
      361 ttgtagagtt tatttttttg ctcatttctg acataaaaaa tccggtttgc aggatgctca
      421 gggaccgaca taccatattt tttattgtaa ccaacgccag gcccagaaat gataacctcc
      481 tgtccgttca ccgatgcctg tacacaatta ttgttcatga ctttttcgat gatcattcca
      541 tggctcccaa aaaaaaaggc aaggcatgtc agaagtaata attacttcaa acattgcctt
      601 gcctgattta acagttacaa gataattaac taatgcgtat accttacacg gaattgtgct
      661 taataaaaga gatgacaggc ttatttttta agccgatatt caaatattcg acagcagtca
      721 caaaaccatc gttcaacatt tatgtaaata atatttattc aatgaaacat aaattaattg
      781 aataaattaa aaacatcatc cccttctcgc cagcgtcgcc ccatcggcaa aatacgcttt
      841 aatccccgca agaatagact ccgcaacttc ctgctgaaag gtcgccgttt tcagtttacg
      901 ctcttcctca acgttactga taaacgccgt ttcgaccaga atggagggaa tatctggtgc
      961 ctttagtacg gcaaacccgg cctgttcaac ctgattttta tgcagcttgt tgattttacc
     1021 gagcttattc agtaccgctt taccaaactt aaggctgtca gcaatggtca gcgactgcac
     1081 catatcgaac atggtgtggt cgacatagcg gtcaccgctt ttgctcacgc caccaatcaa
     1141 gtccgaggcg ttctgggttt gtgccagata ttttgccgca gtactggttg cacctttggt
     1201 tgagagcgca aacacagagg aaccgctcgg ctgacgactg gtaaaggcgt cggcatggat
     1261 agagacaaac aggtcagcac gctgtttctg ggcttttgct acgcgcactt gcaacggaat
     1321 gaagatgtct tcattgcgcg tcatgtacac cttcatattg ccctctttct cgatcagaga
     1381 gcgcaaacgg cgagctattt gcaataccac atctttttct cgcgttttgt atttccccac
     1441 cgcaccggag tcttcgccac cgtggccagg gtcaagcata atgacaatcg gacgatcgcg
     1501 ccctgcttta cccggttgtg gaccactttg tgctggcggc acctgctttt cgaggtcgcc
     1561 tttgttgtaa tcctccagca gcgccagcag cgggtcctgc atatcctgtg cattggccgg
     1621 atagaggtcc atcaccagac gctctttaaa cccggcgacc ggcgcaaggg caaacagctg
     1681 cggttttacg ttttgcttta attcaaaaac catccgtacg gtctgcgggt caaattgccc
     1741 gacgcgcgcc gacttgatga acgggtcgtc tgcacgaatt tgtgccgcca tccccttgag
     1801 taccgagttc aggtttacat cttcgatatc caccaccacg cgttccgggt tactcaacgc
     1861 gaactgctta tatttcagct gacgatttga ttctaccgtc acacgggtgt agctggacgc
     1921 aggccagacg cgcaccgcca cgacctggct gaccgcagcc agactgacct gacttacgct
     1981 caatagccac atggcacccg cgccttgcag taaacgacga cggctgattg cagtgttgga
     2041 tcctgacatg cctctcccga gcaaaagaaa tctaagctgt gtaacaagta aacgactaat
     2101 ttgaccggaa actttagcga aagacgcata atctgtcatc taataaacgg taaacattct
     2161 ttttatattc acggcattac tgataaaaaa gtcgctctcg cataaaattt acacttgcac
     2221 cctgcgaaaa aacagaataa aaatacacta atttcgaata atcatgcaaa ggggtgtgcc
     2281 gtggtaaagg aacgtaaaac cgagctggtc gagggattcc gccattcggt tccctatatc
     2341 aatacccacc ggggaaaaac gtttgtcatc atgctcggcg gtgaagccat tgagcatgag
     2401 aatttctcca gtatcgttaa tgatatcggg ttgttgcaca gcctcggcat ccgtctggtg
     2461 gtggtctatg gcgcacgtcc gcagatcgac gcaaatctgg ccgcgcatca ccacgaaccg
     2521 ctgtatcaca agaatatacg tgtgaccgac gccaaaacac tggaactggt gaagcaggct
     2581 gcgggaacat tgcaactgga catcactgct cgcctgtcga tgagtctcaa taacacgccg
     2641 ctacagggcg cgcatatcaa cgtcgtcagt ggcaatttta ttattgccca gccgctgggc
     2701 gtcgatgacg gcgtggatta ctgccatagc gggcgtatcc ggcggattga tgaagacgcg
     2761 atccatcgtc aactggacag cggtgcaata gtgctaatgg ggccggtcgc tgtttcagtc
     2821 actggcgaga gctttaatct gacctcggaa gagattgcca ctcaactggc catcaaactg
     2881 aaagctgaaa agatgattgg tttttgctct tcccaaggcg tcactaatga cgacggtgat
     2941 attgtctccg aacttttccc taacgaagcg caagcacggg tagaagccca ggaagagaaa
     3001 ggcgattaca actccggtac ggtgcgcttt ttgcgtggcg cagtgaaagc ctgccgcagc
     3061 ggcgtgcgtc gctgtcattt aatcagttat caggaagatg gcgcgctgtt gcaagagttg
     3121 ttctcacgcg acggtatcgg tacgcagatt gtgatggaaa gcgccgagca gattcgtcgc
     3181 gcaacaatca acgatattgg cggtattctg gagttgattc gcccgctgga gcagcaaggt
     3241 attctggtgc gccgttctcg cgagcagctg gagatggaaa tcgacaaatt caccattatt
     3301 cagcgcgata acacgactat tgcctgcgcc gcgctctatc cgttcccgga agagaagatt
     3361 ggggaaatgg cctgtgtggc agttcacccg gattaccgca gttcatcacg gggcgaggtt
     3421 ctgctggaac gcattgccgc tcaggcgaag cagagcggct taagcaaatt atttgtgctg
     3481 accacgcgca gtattcactg gttccaggaa cgtggattta ccccagtgga tattgattta
     3541 ctgcccgaga gcaaaaagca gttgtacaac taccagcgta aatccaaagt gttgatggcg
     3601 gatttggggt aagcgatgaa aatcgccgga tgcgacatgc gtaacactcg tgcgtcgcat
     3661 caggcaatta cgtttattcc cgcgaactaa acaatgccgc cagaccactg cgccgctcag
     3721 tgcgcgtggc gattgccgca cttaatatgc gctcatcggc atacagcgac agacggcgac
     3781 gcgcgcgggt cactgcggtg taaaccagct ctcgcgttac taccggcgtg cgttggctcg
     3841 gtaaaatcaa cgccgcatgg tcgaactccg atccctgcga tttatgtacc gtcatcgccc
     3901 acgtcgtttc gtgctctggc aggcggctcg gttgcacaga cttaatattg ccgtccggca
     3961 tcgcaaacca aacgcgcgtc ccctgcccgc gatccagcgc aataccgata tcgccattaa
     4021 acaacccaag cgcgctgtca ttacgggcaa tcatcaccgg tcgaccttcg taccaacgag
     4081 agtgcggatg acgatgaatt ttgcgcttct gttgcataaa ctgctcaatt cgctcattca
     4141 gtccagccac gccaaacggc ccttcccgca gggcgcacaa aagttggtac tcattgaacg
     4201 cctgaatgat taaatccggc tcggcacgcg cttgcagcag atccagataa cgtccataac
     4261 ccgcaagagc ttcctcaagc atcgcaatat aatcttctcc gctctgtaaa agtcgttttt
     4321 cgatatcagt aaaatcctgc tgaaaaacgg ttttcactgc cgttttatca ccacggttga
     4381 tcgccgcagc taactgacca atgccagaat cgctgccgaa acgatagctt ttttgcagca
     4441 ggcagagact gtcgcgcaaa gatgccgctt ctgtgccggt tcctgccgga acgtgagtcc
     4501 ccgtcaggcg gcttagctgc ctggcacgct cggcggtaaa gcccgcgttg gcataagcgc
     4561 agatatcgcc cagcacagcc ccagcctcaa ccgaggccag ttgatcacga tcgccgagaa
     4621 agatcactcg cgcatgatct ggcaaggcgt cgatcagtct cgacatcata ggcagatcga
     4681 tcattgacgc ttcatctacc accagcacat caagatgcag cgggttaccg gcatgatgac
     4741 gtaaacgctg gctacccggc tgcgcgccca gcaatcggtg caaagtgctg gcatcttccg
     4801 gaatgcgttt cttttgttca tcggtcagcg gtaactgtcg caaagccttg ccgagagatt
     4861 cggttaagcg cgcggcagct ttacccgttg gtgcagccag acggatacgg cagcgttcgc
     4921 cgtcggccat ttgaattaac gctgccagca acttcgccac ggtggtcgtt ttaccggtgc
     4981 ctgggccgcc ggaaaccacc gagatccgcc gcgtcagcgc cactgccgct gcaacttttt
     5041 gccagttaat ttcatcgctt actggaaaaa gtttgtccag ggtttgcgcc agtagagctt
     5101 catcaacctc aatggcatga ttcacctcat tgaagaagcg cgctaccgtc cgctcgttac
     5161 accacatgcg attcaaataa agacgatcgc cacagaggac catcggcgtg ggttcatctc
     5221 ccctgctgac cgcttgagaa gccagcaagc attcttccca attttgtagc tcaccgattt
     5281 cactgacaca ggtcgccaac agcggatgcg acgcctcgtt attttccagt cgtgaaagcg
     5341 gcaaacagac gtgtccctct ccggcatcat gacttaacag tgccgccgcg agggtgacgg
     5401 caggatgttc atctcccgcc acggtcaggg caaattgcac atccagcggg cgtagctgtt
     5461 tgtgctccac agcttccagt aattgctttt gcaatttcat tacgcctcct ccagggtcat
     5521 accggcaaac atctcatcca tcagggcaat caacccggcg ttgggtcggg ttgtgtaaat
     5581 cccttgttgc ggatgttctt tatcaacgcc acgcaggaac agatagataa cgccgccaaa
     5641 gtgacgatca tagtcgtaat cagcaatgcg atggcgcaga taacgatgca gcgccagggt
     5701 ataaagctga tattgcagat catagcggtg tgcctgcatc gctgctgcca tagcctgttg
     5761 ggtgtaagcc gaactgtctt cacccagcca gttggattta tagtcgagca ggtaataacg
     5821 cccttcgtgg cggaatacca ggtcgataaa gccttttaac atgccgcgta cctgcatgaa
     5881 ctccagcggc gggcagccag cggatagcgg gtcaaactgg cgaattagtg catcaagctg
     5941 gctggcgatg agcggttcgc taatcggcag gtaaaactcc atctccacct gtttatcgcg
     6001 gtcggaaagc tgactcaggc taacacccgt ttcattgaga ggtgcctgga ggacagccgt
     6061 gatccactcg gtcaacaccg gttcccactg cgattcaaag ccgccgagtt ccagttcttc
     6121 ctgtacccag ttcgggtcaa ccggctgggt aaaatcgagg tcttcaaaca aactgtgcaa
     6181 gaatgtcccc ggtgacgcac cgcgcgggaa ctgatgcggt gttaacgtcg gttcttcaac
     6241 gacgctgacc acgcctgcgg catcaacatc cagccgtggc atcagatcct gagcgatacc
     6301 gtgaccacgc tgctgcaagc cggagtagct ggtgacacgc cagttatcgc cgggcaatcg
     6361 ttgtagcgtc ctcgcattca gttctgcagt taaagcatca ttaacctgcc agggctggtt
     6421 atcaccagtt tgtgccgttt gccaggcaat atcatcatcg cataacgctt caatacaggt
     6481 gcgaagccct gccgcatctt gcggttcccc tttttgcagc aaacgcccga gcgcactttg
     6541 gtggacgtcg gtgtcacctt ttttatcgcc acgacggcgc accagcggtg caacgccgag
     6601 actgcaatgc caaaccgaac gcgtcagcgc cacgtaaagc aaacgcagat cttccgccag
     6661 acgttcgacc tccgcaagct cgacgctttc tggcgcagcg ttaagatcca gaacagcctc
     6721 aaacgagtgg cgatcgtgat aaaacgcctg atcctggacg cggaaattgg taataaatgg
     6781 caaccagacc agcggatatt ccagcccttt cgatttatgg atcgtgacaa tctgcaccag
     6841 atgtttatca ctttcgaggc gcatttgttg gctggaggca ttactgtctg gctcgaggat
     6901 atgttgcgat aaccagcgta ccagcgcatg ttcactttcc agctgcgttc cggcttcttg
     6961 tagcagttcg ctgatatgca agatatcggt aagacgccgc tcaccgcctg ccgttgccag
     7021 caagttttct gcaatgttac gcgccgacat cagcgcccgc agcatcggca taacgccacg
     7081 tttgcgccag atttgccgat aaccatcgaa ctcttcaact accgcatccc acgcatgttc
     7141 gtcattattc agcgtttcga tatccagcgc gttcagcccc atcattgacg ttgccagcgc
     7201 actgcgcagg gtgttctcac gttcgggcgt catcaccgcc tgcaacaacc aaagcatttc
     7261 ctgcgcttcc agagtttcaa aaacactgtc gcggttcgaa aggtaaacgg aagggatttc
     7321 cagcaacgtt aaggcatcgc gcacctgggc ggcctcctgg cggctgcgca ccagcacact
     7381 gatgtccgaa gcacgcaccg gacgcgcgtc gtcgccgttc atcagcaacg cttcgccccg
     7441 ctgtccggct tgtagccagt cgcggatttg cgcagcacat acctgcgcca tggtactttg
     7501 ataatcgcca acgccgcagc tttcgccttc catcagccac attttcatcg caggctgcgt
     7561 ttcaccttta aacacaaaac gtaacgcctg atttttcccg gctgatttca ctggaataaa
     7621 cggtatttcg cgaaacatga acgcgtcatc agtctggctg aaaagcttgt tcacactgtt
     7681 caccattcca ggtgcggagc gccagttggt gtctaaagtg tagtgggcgt gaacttcact
     7741 acgcgccttc atataagtga agatatccgc accccggaat gcatatatgg cctgcttcgg
     7801 gtcgccaatc agcaacaatg cggtttccgg ctgatggtgc cagatacggc gaaaaattcg
     7861 gtactgctgg gggtcggtat cctgaaattc atcgatcatt gccaccggga atcgcgtacg
     7921 gatcgccgct gccaacacct caccgctttc gctacgcagc gcggaatcga gccgacttaa
     7981 catgtcatca aaacccaatt cgccacggcg gcgtttttca cgcgctactg tttcgcggat
     8041 ctcagccaat gcgcgggtga tcaccagatc gcggatcgac aatggttctg caagcagttg
     8101 atcgatcgcc tcaaacagtg gatgtcgcgg ggtttccccc ccggccttcg tgcgatcttc
     8161 taagaaacgc tgggagaatt tttccagcga ctccggcaac tgataactgt ttgtctcttc
     8221 ttctgcccag gcgctgatct tttcgatcca tttagcctga ttgctacggt taaacttgcg
     8281 tcgatcaata ccagaagatt cgatcagcgc atcaagttca cccactgcgt cgcgccactg
     8341 ctgttttacc gtatcaatac gcgccacaat ttgcgcgtgg cgggaagcca gcgtttcatc
     8401 atcgggcggc ggtgctttga taaccggtgc ttcgccttgc agataacgat taatatcgcg
     8461 cagcaacgcc tgcggccctt tccaggtttc aaagaccacc agggcaattt cacgcggcag
     8521 cgggtagcag tggcgacgcc agaaatcggc gcaggcctgg tagcgtagca gagactcatc
     8581 ttcaatcagc tgctgctcaa acagcatacc ggattcaaag gcattcaggt tgagcatgcg
     8641 ctggcaaaaa ccgtgaatag taaagactgc cgcttcatcc atctgccgtt cggctaacaa
     8701 caaccactgc gcggcttgcg ctttatcgtc gatctcttcc agcaggcgtt cgtacagtgg
     8761 attgtcggtg gtttcacgca gacaggcgat gcgcaactcg tggatattgc tacggatacg
     8821 accacgcaat tctgccgtgg cagcctcggt aaaggtgacc accagcagtt cttcaacggt
     8881 cagcgggcgg ggaaaggcgg cggaaccgcc tagtccaagt aacaggcgca aatagagcgc
     8941 cgcaatcgta aaggttttgc ctgtgccggc agaggcttca atcaggcgct cgccctgtaa
     9001 gggcaagcgc aaaggatcta gtgtctcggc gacatcactc attcttttca ctcatcaggg
     9061 gcattgtttg ctgcaacgcg ctgacgttct cccacacttt ccagccttca ggatgcacat
     9121 attcggcttt cccgttctgg ctgccggaaa tctgcgacag aatagccatg ccttgcggct
     9181 cgaccaccgc ctgatggaag aaatcagcaa gtttttgcgg cgtcagcagt tttatctggg
     9241 ccacgatttt atcacgcgaa tcgaagcgca tattgccgcg atcgaaatct ttacttaact
     9301 tcgatgcttc ttcgccgagc gtttgcggtg cctgcagcat ctgggtaatt accgcctgct
     9361 ggatttgcgc aaactcttct ggcttcatcg cccgcaattt tgcctctgcg gttgggaaaa
     9421 acgccttgta acgctcccac aagaaggaag gctgtttatc attgctttgc aaaaggaagc
     9481 ccatgcccca ctgacgcccc acgctcattg gaaatgcaaa cacggcatag cccaattgtt
     9541 cttcggtacg caactgattg tagaaccacg gctgtacgat ctgccccaac agagagctat
     9601 aggctgagct ggtgtattca tcgtagccag tcggtacaaa taccgctgcc agtgcggagt
     9661 cggtgctgtt accagctttt tcaaagatga cggattgttt tttatcgacc acgacatctt
     9721 tgttacgaca ccactctgaa ccatcagcgc ccaactgttt ttgcacatcg cgtgccagcg
     9781 ttgttgcctg gtcctcggtc atgttgccga taaccataaa ctctggtcga gcccctgatt
     9841 ttaaggcgtc gcgataggcc agcacctctt tcaacgtaat ggagggcaaa attttgcgcc
     9901 gttcatctcg cgagaagtac ggcacttgcg agagcatctg cgcgggcata atcgcctgct
     9961 cgaacgcttt gcccttttct gcggaatcca tcatctggtt ataccaggac ttcgcctgct
    10021 caagctgatc ttccgtggcg gtatagctaa agtagccttc cagcaatgcc tggaacagct
    10081 gcggcagatg ctgggtgtaa ccattagcat taaccataag gccgttgtta gcgttggtgg
    10141 aaaaacttat gccaccaacc gacgcctggt tgcttaactg atcaagcgcc agccctgcga
    10201 gataatcatt gagcgcaaac atcacctgat tgcgggcgct gtccatggct ttcggattac
    10261 gcaaaatcag gctgacatcg gcttttggct cgctggcaaa ataacggctt ggcgcataca
    10321 ccacgcgcag attcgactca tcaacaatca gctctggatg gtcatatttc ttgtctgact
    10381 taatcagcga gaaatcgtca ggaatataag ggttaagctc tggcaaagag agcgcaatgt
    10441 cggcggcttt tttctgccag tcggcgaaag tttgtgcgct gattttatcg acctgatacg
    10501 gcgcatcgac aaagtaagcc gttttgttgt gcggctcttt cgggctgata taccagatac
    10561 gcgcattctg cggcgtcatc atcgccagac gttcctttac tgctttagca tcgtaccgat
    10621 cggcaatatt gactgcatcc agcgtatgct caacaggaac gcgaatcatg gtatctgcca
    10681 gccattcgac gtaatccata tcacgggtga ttgacggata acggaagtcg atatccagca
    10741 cattcgccag ttcatcgaag tattgtttat cgatcccttt ttcacgtaac aaattgaggt
    10801 agctaaaaat agccgccaca acctgatcgc gattcgccag acctttatcg gttaaagacg
    10861 cagagatcgc taatacgccg ctgttgccgt tgacgatagg atcggagttg gcgctaatgc
    10921 cctcaactaa tccctgcttt tgcagccagt cggaaagtgt acctgggctg cgattaccaa
    10981 tcagataggt aatcaattca tcggttttgc tacggaattt cgccgagttg ttatcaatac
    11041 gaaactctac gcgcaacact ttacgcggca gcgccgggac gtaatgaatg ataatgccct
    11101 tttgcgcgtc ggtgactacc ggcacggtga tttccggttt tttgctctct ttgttcggca
    11161 cgcgaccaaa ggtgtccgcc gccatttttg ccaactccgg cagcggttta ttgctgtaaa
    11221 taaccgcctt catcagattg gcggaatagt acttctcgtg gaaatctttc agcgcctgct
    11281 gcaccggatt accaggtttg tcgcttaaag tttcgaggtt accaccagaa aactttgaac
    11341 cggggtgtgc cgggttaatg gtttctgcgc tgacctgtgc catgcgcatc ccgtcacgcg
    11401 tacgcgccat ggttaattca gcgttcactg cattacgctc acgttcggcg tatttcttgt
    11461 cgagcaaggg ttctgcaata gcatcagcca ggcggtctac cgcaccaggc aaggcgtcgt
    11521 tctcaacttc cagatagaaa gccgtgcgat acggcgcagt gctggcattg tgactaccgc
    11581 cgtgcatttt gagatattcg gccagactgt cagcctgcgg gtactttttc gaccccatca
    11641 gactcatatg ttcaaggtaa tgtgccagcc cctggtacgc ctcgggatct tccagcgacc
    11701 caacgggcac caccagcgcc gagagcgatt taactgcctg cggatcagaa accagcaaga
    11761 ccaccatacc gttatccaga cgtatagcct gatactggcg gttatcttta tcacttttac
    11821 ggatggtttc ctgaatcggc tgccatcccg tttctgcctg acttaagggt gcccaaaggg
    11881 caactaacaa caataatgct ttgaaccagg tgctgcgggg cattcacgga cctcataatc
    11941 aacttaattt tctgtccaga ttcaacacgt taacgcaaat catctgccag aatttaatct
    12001 tgtgctgcac gagtcagcct atgtttatat aaccatcagt ccgtgactgg tgcgcatcat
    12061 aaagtaagcg gatagattgc gcaattttta tacagcactc atgactgatt aaagcgaaac
    12121 agcggtaaca ggaaacgttg cgactgttca acgatggcct ccattgtctc tggtgttaat
    12181 tgccgccaga gcctttgata ccagatatca tcaccttcgc cacgcaccat catgttgcct
    12241 tcgtaagcct gaaggaattt cgtacgggct ttttgcaacg tggaatcgtc atccagcatg
    12301 gcatcgtttt gcgcgtcata acaggttttt agccacgcgc cgccactttc aggtaacacc
    12361 agcaatggcg cggacatccc ttcacgatac ccctcaatca gttgtgagag gtaatgcaaa
    12421 gcctgttcgg ctgcaagcgg cggaaaacgc cactcgccgt ctttgcgtag aaaaaggcga
    12481 ctttcaccat taccaccgct ggcacagtag acaaggtgtt ccagccaaag ttgcattccc
    12541 tgcgccacac ttaataaaga gggacgccag cgcaacaggc catccggctg cacctgcggc
    12601 aaccagccag ttatctgcac accgttgcag gcgagatcaa tttccatact ctgccccggc
    12661 tggcgacagg caatgactct gtcggcaagc tgctgcatct cctggcactg tgtttcccag
    12721 aaaatttcac caaaagcgcc atacggtaaa tcccctgccg cccggaagcg gcggaacaag
    12781 cgttcggcat catcctgctc aaccagtgca ttcaataact gctgattgat ttgataacgg
    12841 ctaagccctt ccagaataaa tggctcggtg tctgggattt cgctgtcttc agtacggaag
    12901 ttcacctgca aacgcatctg gaagaacgcc cgcaccggat gtgcccagaa tcgttgtagc
    12961 gtttccagcg gcacggtttc cggtaaggta aacggcagcg gctgaacaaa ttcagaatgt
    13021 gctttaccag cctggctggc cgcaggtagc cattcacgag catagctttg tcgttcgcct
    13081 ggctggtagt tttgcggatc aaacggcatc cgggtatgga ggcaagtaag atgcgctttt
    13141 acccttgcct cgctttcatc acagttgagc gcttcatcgt ccggtagata atgactttgc
    13201 ccgatgtagt cgatcagttc ctgcaccagt accgacggga aacgttcact gttatcctga
    13261 atggaacgcc cgatatagct gatatagagt ttttgctgcg cggaaattaa cgcttccagg
    13321 aacaggtagc ggtcgtcatc gcgacggcta cggtcgccac gcttcggttt ctggctcatc
    13381 aggtcaaagc ccaatggcgc aagctgacgt ggataaacgc cgtcgttcat tcccagcagg
    13441 caaaccactt tgaacggaat tgaacgcatt ggcatcagag tacaaatgtt aaccggtccg
    13501 gcgagaaaac gctggctgat acgttcttga tccagacgct gcgccagttc atcacgcaat
    13561 agtgacagcg gcaccgcgtc gccatactgc gcacctaaac cttcggcgat aatcgcctgc
    13621 cattgttgtt cgatcagcgt catcgccgct tcggtttccg catccggcag gaagaaggcg
    13681 ttgagcatat cgcgacaaac cggcaaccac tcttctagcg gacgctcctg tgccagcccg
    13741 cgacgccaga tgtttagctg cattagcagt gaagccagat gccccaccag ttctgcaatt
    13801 aagccgctcg attcatcata aggtagaacc gattgccact cgccctgcgc gctctccatc
    13861 gcgtagccca gcaacatacg cgtcaggcca aatcgccagg tgtgttgtcc ggtggcgggg
    13921 agttccagct cgcgaacgtt gtcgtcatct atcccccaac gaatgccgga ttcgttgacc
    13981 cactggcgta aataacgcag cccttcttcg gtgatgtcaa accgcgccgc cagcaccggc
    14041 acatccagta atgccagcac gtcttccgac acaaagcggc tgtctggcaa cgataacagg
    14101 ctgataaacg cttccagtac cggatgcgac tgccgcgccc gacggtcgga aatggcgtaa
    14161 ggcaggtaac gatccgcagg tgcgctacca aacacagcct gaataaacgg actgtagctg
    14221 tcgatatcag ccaccatcac gatgatgtcg cgcggagtaa gtgtcgggtc ttcttccagc
    14281 atcgccagca ggcgatcgtg caaaacttca acttcacgct gcgggctatg gcaaacgtgg
    14341 aaggtgatac tgctatccag tggatcaagc gggcgtttgt tatcgctacg ggaaaactct
    14401 tcgatgttca caccagcaac ggcacggttt tccagttcca gaatgtcaga ctgaatgtta
    14461 tgcagcaggt tatctggcgt gacatcgaca aaggcgtcca gctcctggct gctctccagg
    14521 tcagaaagga gataaatgta gtcgcgccca agcttacccc atgaagccag cagcgggttg
    14581 ccgacatcct gttcaccatc gctgttaaag agctgcccgg cattttcgct atcacgaaat
    14641 agcggcaatt cgcgatcttc aacactgtgt cgacgctgac gggtcagcag tttcgccaga
    14701 taagcaggat ctttaatatc gccccagtaa taacggcagg ggttggtaaa caggagatgg
    14761 atttcaatat gtttacccag cgcctggagc gcctggagat aaacaggcgg taacgcggaa
    14821 ataccgcata taaagacgcg cgaaggtaac cccggcgggc aggtcgtcgc ggactccagc
    14881 gtttcgataa agcgctgata gagattggcg cggtgccaac gcggttgtcc gagttcatgg
    14941 gtatattcca ccagcgcctt ccacaacggc gcttgccagg cctgtgcttc tcccagccct
    15001 tcaaccaaat gtcctgtttc ccactgtgcc agccactccg ggcgatagac cagatactgg
    15061 tcaaacaggt ccgccgcttt tgaggaaagc tggaacagtt ttcgcttgtc gctatcgtca
    15121 gccagataat gccgcaacag ggtaaagtct tcgcgatcca gcaattgcgg cagcagagtc
    15181 atcagtttcc agctcatgct ctgtttgtta aaggcgctct ctttggggat ctccggtaac
    15241 acccggacga acatatccca gataaagctc gctggcagcg gaaaatcaat gtttgccgca
    15301 ataccaaact tttgcgacag ggtcatttgc agccactgcg ccataccggt actttgcacc
    15361 agaatcatct ctggttcgaa aggatcgtcc agccgttcgc gttcgacaat aaactccatc
    15421 aacgcttcca gcacgtccag acgattggaa tggtagaccc ttaacatagc ggctcctgac
    15481 tactgacgat tcgggcaatg caggcgggtc atctcgcctt ctctgccccc gggtgaaact
    15541 agcgtaacgc tgatgctgac acatcccgcc tgcgatgtct gcattcggtt gacctgccag
    15601 ttggcaggtg gcgaaatcgc gcgcagttgc gtttgctgcc aaccatgccg ccagagctgt
    15661 tggtactggt ttctgctggc gagactgttc attaatgttc gctggatacc cgataacgca
    15721 gtgacaatca tcaccatcag caccatcgca accattacct ccggcaggct aaagccttgt
    15781 tgattcttca gggaagctga cataacgccc tctctttcag cggacaaaaa tcgctccagc
    15841 cgcgtggcga aaaaacaatg tttccatcaa tgacttcgcc tgttcgccac aacgaaacgc
    15901 cttcataacc ggcaaccaat aaggcttcat tatctgccag taaacgcaaa caaacccggg
    15961 catcggttcc agcgtactgc gagcactgaa ctgcgggctg cgtctgccag gagtgcattt
    16021 tcccccacgc cagcgccgac tgaacgatgg cctggcggcg caatgactga ctttccatgc
    16081 tcacgcgaga ggcaaaactg cgatcctgct gactcattcc ttgcaatagc aagctaccca
    16141 aaaccagcag catcaggacc agagccagtg acgaaacacc tttttcgcgg ttcacaggtt
    16201 gaatcctgtc acgctatagc tggcatccac cacggtttgc ggttcagact tactggcagc
    16261 acgcatatta accgtcaaca ccggcgagaa gccgctgaca tcctgacgta cgacctgaaa
    16321 agtgtcgata atgatggcat ccggattagt gactttatcc cagcccttac cttcacagga
    16381 tgtcgcaccg cgtagcgttt ccagcacatg ctccttcaga cgaaatccaa tctggtcaga
    16441 ctcttttacc ggttcgcgat cccagatacc gttactgttc gcatcccact gcacaatgat
    16501 acagtcaccc tgtccgacaa tttccagccc ttcgccggta cagctgccat gacaataacc
    16561 cgccctctgg agatgcttcg cgacggtaaa tacccgcagc cagatttcat cttccagcgc
    16621 cagcttacgg gtgctcgtta aactttcacg ctgtaacgca ggcagaaagc gtgcagcccc
    16681 cagcaacaat acgctactga tcgccatagc aatcaacact tccagcagag aaaaaccttg
    16741 ctcttttaca ggcatccttc tgtttctcct tgctgacaaa gccggagtct tccccacggc
    16801 gaaaccacca gccaccactc gcccgttgag tttttgaagc gaatatgccc ggcccatgcg
    16861 gtattgcgca ggccaaagaa agcaagcgaa ggtgtcaggt cgctcatttc gacttcgggc
    16921 cagcgtggca caaagaccaa tggtgaactg ccatgacagg tattggcccc agcagcggaa
    16981 ctcacaaggc accataacgt cccctccctg ataacgctga tactgtggtc gcggttatgc
    17041 cagttggcat cttcacgtaa atagagcaaa tagtcccgcg cctggctggc ggtttgccat
    17101 agccgttgcg actgctgcca gtattgccag ccatagagtc cacttgcgct tagcatgacc
    17161 aaaatcagca tcgcgaccag cgtttcaatc agcgtataac cacgttgtgt tttcatgccg
    17221 gcagtatgga gcgaggagaa aaaaagacga gggccagttt ctatttcttc ggcgcatctt
    17281 ccggactatt tacgccgttg caggacgttg caaaatttcg ggaaggcgtc tcgaagaatt
    17341 taacggaggg taaaaaaacc gacgcacact ggcgtcggct ctggcaggat gtttcgtaat
    17401 tagatagcca ccggcgcttt aatgcccgga tgtggatcgt agccttcaat ctcaaagtct
    17461 tcgaaacggt agtcgaagat ggattcgggt ttacgtttga taatcaactt cggcagcgga
    17521 cgcggttcgc ggcttaattg cagatgagtt tgatccatat gattgctgta caggtgcgtg
    17581 tcgccccccg tccagacaaa atcgcccact tccagatcac actgctgcgc catcatatgc
    17641 accagtaacg cgtagctggc aatgttaaac ggcaggccga ggaaaacgtc acaggagcgc
    17701 tgataaagct ggcaagagag tttgccgtct gccacataga actggaagaa cgcatggcac
    17761 ggtgccagcg ccattttatc cagttcgcct acgttccacg ctgaaacaat aatgcggcgc
    17821 gaatccgggt cgtttttcag ctggttcagt accgtagtga tctggtcaat atgacgacca
    17881 tccggcgttg gccaggcgcg ccactgttta ccatacactg gcccgaggtc gccgttttca
    17941 tcggcccatt cgtcccagat ggtgacattg ttttcgtgta gataagcaat gttagtgtcg
    18001 ccctgcagaa accacagcag ttcatggatg atggaacgca ggtggcaacg tttagttgtc
    18061 accagcggga atccatcctg caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt
    18121 ccggttccgg tacggtcgtt tttctgtgtg ccttcgtcga gcactttttg catcagttct
    18181 aaatactgtt tcatggttcc tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag
    18241 accatcatga tcacacccgc gacaatcatc gggatggaaa gaatttgtcc catgctgatg
    18301 tactgcaccc aggcaccagt aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg
    18361 cgaaacgcgc cgtaaccaat caggaacaaa cctgagacag ctcccattgg gcgcggttta
    18421 cgaatataca ggttgaggat aataaacagc actacacctt ccagcaacag ctcgtaaagc
    18481 tgtgatgggt ggcgcggcag cacaccgtaa gtatcgaaaa tggattgcca ctgcgggttg
    18541 gtttgcagca gcaaaatatc ttctgtacgg gagccaggga acagcatggc aaacgggaag
    18601 ttcgggtcaa cgcggcccca caattcaccg ttaataaagt tgcccagacg cccggcacca
    18661 agaccaaacg gaatgagtgg tgcgataaaa tcagagacct ggaagaagga acgtttagta
    18721 cggcgggcga agataatcat caccacgata acgccaatca ggccgccgtg gaaggacatg
    18781 ccgccgtccc agacacggaa cagatacagc ggatcggcca taaactgcgg gaagttgtag
    18841 aacagaacat aaccaatacg tccaccgagg aagacgccga ggaagcccgc atagagtaag
    18901 ttttcaactt catttttggt ccagccactg cccggacgat tcgcccgtcg tgttgccagc
    18961 cacattgcaa aaatgaaacc caccagatac atcaggccgt accagtgaag cgccacgggt
    19021 cctattgaga aaatgaccgg atcaaactcc ggaaaatgca gatagctact ggtcatctgt
    19081 caccacaagt tcttgttatt tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt
    19141 ttcaggcgct ccaaaggtgc gaataatagc acaaggggac ctggctggtt gccggatacc
    19201 gttaaaagat atgtatatga tccgcgctat aaccctccgc gaatcagccc gcccatgcca
    19261 cgacgctcca taaaggctgc aacctgatgg cgaacttcgg tcgccagttg cgcttccaga
    19321 ctacgctgcg caagattttc tgcttcggca taatcaatgc gccgcagcag gtattttacc
    19381 cgcgctacag aacgtccgtt catagacaga tggcgatacc caagcccaat gaggattgcc
    19441 acgcacatgg gatcgcccgc catttcaccg cacaaacgga gatcgattcc atgtatttcc
    19501 gcttcccggg cgatcatcgc cagagctcgt aacattgcag gatgaagact gtcataaatg
    19561 ttcgccaccc gggtattgtt gcgatcaacg gccagaatgt attgagtcag atcgttggtg
    19621 ccaacagaga tgaaatcgac ccgctttgcc agatgcggca gcataaatac cattgacggc
    19681 acttccagca tgatgccgat acgtggtttg ggaatttcgt aaccgatcat ctcctcgact
    19741 tcacgtccgg cacgttcaat caggcggcgt gcttcatcaa cttcatcgag gcttgtgacc
    19801 atcggcaaca gaatattcag gttgcccgta gcggcattag cacgcagcat cgcccgcacc
    19861 tggatcaaga agatctccgg ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat
    19921 ggattctctt cgctgatcgg catgtaaggc agctgcttat ctgctccgac atccagcgta
    19981 cgcaaggtga cgggtttatc attgaacatt tgcagcatcc cctgatactg cgccacctgt
    20041 tcttcttccg acgggaaacc actttgcagc atgaatggga tttcagtgcg ataaagaccg
    20101 atgccatcaa tacggctgcc cagtttttct tcatgttccg ggcttaaacc agcattgagc
    20161 atgactttta tacgctcacc gctttttaac tgcgcgggta aattgacgtc atcttccgcc
    20221 agacggctaa gctcaatctc ttcactaatt agccgctgat attcttgcag cagtaccggc
    20281 tccggatcga ccagcaattc accgcgatag ccatcaacga tcagcgtcct acgatgcagc
    20341 accgaaggct gaatatccgc gcccatcacg gtagggatcc ccagcgcacg taccatgatc
    20401 gcagcatggg agttggctgc gccatctcgc acgacaacac cgactaagcg atcctggggc
    20461 agctcagcaa gagttgtcgc tgacagttca tctgccacca gaatgaaacg ttccggccag
    20521 gcgttcggcc cttgattagc gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt
    20581 aaatcgccag cccgctcttt gagatagtta tcgcttagcg cggcaaactg ttcggcaaat
    20641 ttttcaatga ccgtttttac cgcccactct gccaccgagc ctttatcaac ctcggcaaac
    20701 aattcgcgac gcagccgggt gtccgaaagt aggtgcgagt aaagatcgaa aatagccgcc
    20761 gtttcttttt gtgctccggc ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct
    20821 tcttccagcg ccccggtcag tcgttcgcgt tccagagccg gatccagcgt tgatgcctga
    20881 tacacctgtt ccattaaagg taacgtggca tcctgccagc cttcggcaat cgccacacca
    20941 ggtgctgccg gtaatgcgcg gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac
    21001 tgcgactggg aaagaatagc tgccatctgg gtggcaagcg tcaccaggaa ggattcttca
    21061 ctttcgtcat actggcgcaa ctctcgttgc tgtaccacca gtacaccaag caactggcga
    21121 cgttgaataa ttggtacgcc taaaaacgcg cggaaacgtt cttcttttac ggaggggatg
    21181 tatttgaagc tggggtgctt ttgcgcatct gcaaggttta tcggttccgc cagcctgcca
    21241 accaggccga cgatcccttc atcaaacgcg agcgttacag tgcgaccgcg tggttttttc
    21301 agcccccggg tcgccatcag gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa
    21361 cagacctcgg tatccatcgc aagacagatg tcggtaacca gaatatttaa cgcctcattc
    21421 aggcgtggtg cgctggctac cttttcgact atttcgcgca ggcgagtgag cataattggc
    21481 gtgacttaac ctcttttacg tcgataagca gatgcgtttt gtggttttgg cgtattttcc
    21541 tgcagtgaca tcaccacact cgcgaactct ttcattaccc tacggtagac atcacgttta
    21601 aatgacacca cctgtctgac cggataccag taacttaccc atcgccagcc gtcaaactct
    21661 ggtgtactgc tggtttgcat attgatttct gcatcgccgc ttaccagctg caagagaaac
    21721 catttttgtt tttggccgat acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt
    21781 aatttgtagc gcaaccagtt acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat
    21841 cctacttctt caaacaattc acggtacatc gcctgctctg cggattctcc ggggttgatt
    21901 ccgccttgcg gaaattgcca ggagtgctga ccaaatcgcc gggcccacat tacctgcccc
    21961 tggcgattac aaatcacgat acctacgttt gggcggtagc catcgtcatc aatcaccgga
    22021 ctacctcaaa ataaagcttt atatacgaat gattgtttca tactccagga agacggtaaa
    22081 ccactctctg cagggcatta cacactaata acaattgaat aactcacagt tatgtgcaga
    22141 gttataaaca gaggaagggg tggatagccg tttttattca ccttttctgt ggatagagtt
    22201 gtgaagaact acggaattac tacgggaaaa cccggagcat tctgaataag cccgatatag
    22261 acattttaaa atattcatta ttagtcaatg cattacatgt aatttgagta atgaaaaaga
    22321 gtaattcgtg acccagctca cacctctcat ttacgggttg gcgaaagatc aaccaatgcc
    22381 gtatttatcc acagaatgtg ccactaagtt aagcactgaa ccactaaaaa ctggagtttc
    22441 gtcgcacgtc aaggctgtaa atggaaacag tagtggaggt ttttcacagt tatcccagct
    22501 ttctgtggat aacatggtgt aagatcctgt ttattttcag tgaccagatt tggaaaacct
    22561 attgcagtgt tgcgcaactc gattaccggc agcctaaaaa gcaatataaa tcagagaatt
    22621 gaacaacaca tatggaaaaa gttacactgc gaatattcgg cacataattg ctgtttgttt
    22681 tttaaccaag gtatcatgac atgtcccaac ctcgcccact gctctctcct cccgaaactg
    22741 aagaacagtt gttagcgcaa gcacagcaac tttctggtta tacattggga gaactggcgg
    22801 cacttgccgg gctggttacg ccagagaatt taaaacgcga taagggctgg attggcgtgt
    22861 tactggagat ctggctaggt gccagcgcag ggagtaaacc tgagcaagat tttgctgctc
    22921 tgggcgtgga acttaaaact atccctgtgg atagtcttgg tcgtccgctg gaaacaacat
    22981 tcgtttgtgt tgccccgtta acgggtaata gcggggtgac ctgggaaacc agccatgtgc
    23041 gccacaagct caaacgcgta ctgtggatac cggttgaagg cgagcgcagc atcccgctgg
    23101 cgcagcgtcg cgtaggatca ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc
    23161 tacgcgaaga ctgggaagaa ttaatggata tgattgttct cggtcaggtt gagcggatca
    23221 ccgctcgtca cggggaatat ttacagataa gaccgaaagc agcgaatgcg aaagcgctta
    23281 ccgaagccat tggtgcccgg ggcgaacgga ttctgacgct gccacgcggc ttttatttga
    23341 agaagaattt caccagtgca ctactggccc gtcattttct gatccagtag ccatcgcttt
    23401 gacctgccgc tttccgggca tataattacc gcttcatttt tttggcaggg ctttttagat
    23461 gttatttgca tggataaccg atcctaacgc ctggcttgcg ctcggtacgc tgacgctgct
    23521 ggagatcgtt cttgggatcg acaatattat tttcctttct ctggtggtgg caaagcttcc
    23581 cacagcacaa cgtgctcatg cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct
    23641 ggcgctgctg gcatcaatcg cctgggttac gcgcctgaca aatccgcttt ttacaatatt
    23701 cagtcaggaa atttctgccc gtgatttgat tctgcttctg ggtggcttgt tccttatctg
    23761 gaaagccagc aaggaaatcc acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg
    23821 cgtttcatca ttcctcggcg ctatcgtgca gattatgctg ctggatatta tctttagcct
    23881 cgactcggtg attacggctg tgggtctgtc agatcacctg tttattatga tggcagccgt
    23941 ggtgattgcc gtaggcgtga tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg
    24001 ccatccttcg gtaaaaatgc tggcgctctc tttcctgatt ctggtgggct ttaccctgat
    24061 tctggaaagt ttcgacatcc acgtaccgaa aggttacatc tacttcgcga tgttcttctc
    24121 tattgcggtt gaaagcctca acttgattcg caacaaaaag aatccgctct gatactccgt
    24181 acgctctcct gcgggagggc gtactcactc cttttaccct cacagtttaa gattttcctg
    24241 ctttcaagat atataacgtc ggtttataaa cagactatta tcataggtag gccaaatgag
    24301 gataaccgga tgaaaaaatg ggcagtaata atttctgcag tcggactggc gtttgctgtt
    24361 tccgggtgtt ccagtgatta cgtcatggcg accaaagatg gccgtatgat tttgaccgat
    24421 ggaaaacctg aaattgatga tgataccggg ctggtgagtt atcacgatca gcaaggtaac
    24481 gcgatgcaaa ttaaccgtga tgatgtttcg caaattattg aacgttaaca aataaggtca
    24541 gcatccggct ggccttaaga tttttctctt tcccttttcc cttccctctg ccatttttat
    24601 attccttatg tcgtgattat aaaaaggaaa cggctatgca atatcaccgt ataccccaca
    24661 gttcgctgga agtcagcacg ctggggcttg gcacgatgac gtttggtgaa cagaacagcg
    24721 aagccgacgc ccacgcacaa ctcgactatg ccgtcgctca gggcattaac cttatcgacg
    24781 ttgccgaaat gtacccagta cctccgcgcc ccgaaacgca agggttaacc gaaacctacg
    24841 tcggcaactg gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc tccaaagtga
    24901 gcggaccgtc gcgcaataat gacaagggca tccgcccgga tcaggcgctg gatcggaaga
    24961 atatccgcga agcgctgcat gacagcctca agcgcctaca gactgattac ctcgatcttt
    25021 atcaggtgca ctggccgcag cgcccgacca actgcttcgg caaactcggt tatagctgga
    25081 cggattctgc gcctgcggtt tcgctgctgg atacgctgga cgcactggca gagtaccaac
    25141 gcgcgggaaa aattcgttat atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct
    25201 acctgcatct ggcggacaaa cacgatctgc cgcgtattgt caccattcag aacccttaca
    25261 gtctgttaaa ccgcagtttt gaagtaggtc tggcagaagt cagccagtat gaaggggtcg
    25321 aactgctggc ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg
    25381 caaaacccgc tggcgcacgt aatacgctct ttagtcggtt cacccgctat agcggtgagc
    25441 aaacgcaaaa agccgtcgcg gcgtatgttg atatcgccag acgtcatggc ctggaccctg
    25501 ctcagatggc gctcgcgttt gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg
    25561 caaccacgat ggatcagctg aaaactaaca tcgaaagttt gcatctggag ttaagcgaag
    25621 acgtattagc tgaaattgaa gcggtgcatc aggtttatac ttatccggca ccataagatt
    25681 aacgccggtt aaaaccggcg ttaaatatta atgacggcgc tgccagatcc acagcgccgt
    25741 tattgccagc gcaaacagcg caccgaagcc aatgccaatg ggcacgaccg ggatgcctat
    25801 cattaccgcc agcgagtaaa tgcccagcat caacaacatg gcgctgtttt cgccaaggtt
    25861 ttgtactgca atcgcattcc ccgccccgac gcttttttta ccccgctcct gtagcaacgc
    25921 attgagcgga acgacaaaaa agccccccat cacgccaatc agcatcaaca aggcataggc
    25981 tggcagcagc tcgtgttgca gggaaaaaat cagtaccacc acgccaatca aaatcccggc
    26041 tggcatacag cgtgacacgg tttccagcgt aactaacttc gctgccgcac ctgcgccaac
    26101 cacgatacca atcgctacca tcgcgttgag ataggtgggc gtagcgttat cggtaatgcc
    26161 cagcgccacc ggtacccaca gcaccaacag gaaacgcagc gtgacacccg ctccccagaa
    26221 taaactggtg cccaccagcg aaaaacgcgt ttcaccattg cgccatagcg aggtgcaggc
    26281 attcaggaaa ctgcgggtca tgttgatgag attccaggac tgccccggac gcgccgccgc
    26341 cagtttggga atgtagatat tggcaacgac cgcaccaccg taggccagtg cgcatgcggc
    26401 cagggcgacg aggacatgcc agtcagccag cacaccaccg gctacggaac cgagcaaaat
    26461 cgccgctatg gtagaagctt ccattaaacc gttagctttc actaacttac tacccgtggt
    26521 taattcgccg agaataccgt atttcgccgg tgaataggct gcagcaccaa cacccaccag
    26581 cgtatagccg agaaacggat tgataccaaa gcagatactg gctgcgccca gcagcttcag
    26641 gccgttggca aacatcatca cccggccttt ggcgaagcta tccgccacct gcccgacaaa
    26701 cggcgcaaaa agaatgtaag cacctacaaa caccatttgc aggatgggct ggctccactc
    26761 cggatagaac tgcgctttca gtaacgccag agtggcaaac agtagggcat tatcgccaaa
    26821 cgcagagaga aactgcgcca cgataaccgc tttcatcccc ttcgaccaca acgaagtgtt
    26881 agtgtgcact gactcactca tcgtgttgtt ccgcttcgtc tacccagctt ttcaacgtga
    26941 caaagtcagg tttgccgctg ccaagtaatg gcatctgttt cagatagcga atatcgcgcg
    27001 gtacagcaag ctccggcacg ccgtgctcgc gggcatactg ttgcaactta tcgcgcgtca
    27061 gttcgttatc tgtggtgaaa agcaccagtg cctcgccttt gctggcatcg ctcttaatcg
    27121 cagtggcatg gactttatct ggcgaaacac caagtgccag ttgttccacc atttccagcg
    27181 acaccatttc gcctgcaatt ttggcaaagc gttttgcgcg gccctgaatc tgcacaaagc
    27241 cctgctcgtc aaaacgcaca atatcgccag tgtcatacca gccgcgctcc atttcgccgc
    27301 gaacattctc ggcggtgggc acttccagta cacctggctt ctccacccgc agatagccgt
    27361 tcattatgtt cggccctttc agttgcaggc gtccgccctc ttcgatacca ggaaccgaca
    27421 acaggcgcgc atccattcct ggtagaatac gccctaccgt accgggtttc gccgccatcg
    27481 gtacgttgat agaaacgaca ggcgcgcact cggttacacc atagccttca aggatgcgca
    27541 ggccaaattt atcctgccaa agctgtttgg tactttcttg tagtttttct gcgcctgcca
    27601 ccacatagcg tagacgatag aagtcatacg ggttggcgaa acgcgcgtag tgaccgagga
    27661 aagtcgaggt accgaacaac acggtgcaac tgcggtcata caccaactcc ggcacaatgc
    27721 ggtaatgcag cgggcttgga taaaggaaca cttctgcacc tgtaagcagt ggcgtaaaca
    27781 ggcctaccgt cagcccaaag gagtgaaaca gcggtaatgc cgacataaag cgatcgttgg
    27841 tggtgaagtc ggcaatcgtt ttaatctgct cgacgttcgc cagaatgctt ttatggctat
    27901 ggacgacgcc ttttggatgg ccttcagaac cggaggtaaa gaggatcagc gcctcttctt
    27961 ccggctgctg ttttacctgc gccaggcgcg gcatcagcaa atgagcgaag atccatactt
    28021 tgtcggcagt ggtgacatct gcttttaaat cttccagata gacccagcgc acctgagtaa
    28081 gttgctccgg cagatgccag agtttgcctt tatcgagaaa ctggcgggaa gtgaagatgg
    28141 ttttgatttc agccgccgta atagcactgg tcagcccttt taccccagca gtgtagttca
    28201 tcattgcggg aatgcggcga cgcgcgatgg ccccaaaaat cactgccgca ctgatgcctg
    28261 cattgggcag cattaagccg atgcgttcgc cttcaacact gtatttttca aggatgcgtc
    28321 caacaaacag cgttttcgta agcaatttgc gataggagtc tggggtaaag ttgacgtctt
    28381 cgacacattt cttcccggct ccgaagcggt acattgcact cagtaaagat tcgtacagag
    28441 tttcacgcgg gcgcaccgcc atacgcgctt ccatcattat ttgatgcagc atttcgccag
    28501 cgattttgcg acggtcacgg gcacgcggcg catccggcat cgccacctgc gttggtggca
    28561 aaatatgcag agtaatttgc gggaacaagc gacgtttaac cagacctttc aggcggctga
    28621 agtgcgtaag ttccgcccct tcaatacgca caggaataac cgttgcacca gacttcgccg
    28681 cgacaaaacc cgcgccatcg tagattttca tcagcgagcc tgtagtggtg atgcgtcctt
    28741 cagggaaaat caccactggt cggccctgtt ccaccagacg taccagatgt ttaatagcca
    28801 taggttgcgt cgggtcgaga ggaacaaagt cgataaatga tttcagccaa cgcatatacc
    28861 actgttggct tattgaggtg taaacggcaa acactggacg cacaggtaaa aacagtccaa
    28921 gcaaaatgcc atcaataaaa gagacgtgat taggcgtaat tagaacgcgc tcgcccttca
    28981 gtgcctgggt gtcacccgta acgcgaacgc gatacaaaac acggcacaaa tttcgaaaaa
    29041 agctaaaaag cattccaact ccctttgctc tgattcagta aaagcgaatg gagggagatt
    29101 acacgagata aagaacgcga gcgacagtaa attaggtgcg aaaaaaaacc tgcgcatccg
    29161 cgcaggttgg tgcaagagac agggtacgaa gagcgtaccg aataatctca ccaatcaata
    29221 cctctgggat cttgattgtg gtctgcacga cgactcttcg ccagcgagaa aacgcaaagg
    29281 aatgaaggga aatgcaacga ggtgtgtaaa ttgtcggtta ctgttacaga ttgatgaccg
    29341 gcaaaaaaaa acctgcgcat ctgcgcaggc tggtgtaatt catgtgctca acccgaagtt
    29401 gacttcacct atcaatacct ctgggatcac cactttagca acctgaagcc aaacgccacc
    29461 agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt
    29521 gtcttactta tttgcgtttg gctcgcattc ccacgatgaa aacacgccac cccttgaacc
    29581 aacgggcgtt ttccgtaaca ctgaaagaat gtaagcgttt acctactaag gtattttcat
    29641 ggcgaccata aaggatgtag cccgactggc aggcgtttca gtcgccaccg tttcccgcgt
    29701 cattaataat tcacctaaag ccagtgaagc ttcccggctg gctgtgcata gtgcaatgga
    29761 gtctcttagc tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac
    29821 ggtcggtctg atcgttggtg atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt
    29881 cgaacaggtg gcttatcaca ccggtaattt cttattgatt ggcaacggtt accacaacga
    29941 acaaaaagag cgtcaggcta ttgagcaact gatccgccat cgctgtgctg cgttggtcgt
    30001 ccatgccaaa atgatcccgg atgccgattt agcctcatta atgaaacaaa tgcccggtat
    30061 ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc ttgacgatcg
    30121 ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa ggtcataccc gcattggtta
    30181 tctgtgctct aaccactcta tttctgacgc cgaagatcgt ctgcaagggt attacgatgc
    30241 ccttgctgaa agtggtattg cggccaatga ccggctggtg acatttggcg aaccagacga
    30301 aagcggcggc gaacaggcaa tgaccgagct tttgggacga ggaagaaatt tcactgcggt
    30361 agcctgttat aacgattcaa tggcggcggg tgcgatgggc gttctcaatg ataatggtat
    30421 tgatgtaccg ggtgagattt cgttaattgg ctttgatgat gtgctggtgt cacgctatgt
    30481 gcgtccgcgc ctgaccaccg tgcgttaccc aatcgtgacg atggcgaccc aggctgccga
    30541 actggctttg gcgctggcgg ataatcgccc tctcccggaa atcactaatg tctttagtcc
    30601 gacgctggta cgtcgtcatt cagtgtcaac tccgtcgctg gaggcaagtc atcatgcaac
    30661 cagcgactaa ccgcagttaa agcaattcca gcgccagtaa ttcttcgatg gtctggcgac
    30721 ggcgaatcaa ccgcgcctga ccattatcaa acagaacttc tggtaacagc ggacggctat
    30781 tgtagttgga tgacattgat gcgccatatg cccctgtatc atgcagtacc agataatcac
    30841 ccgccttcac ttccggcaag gcgcgggttt caacatttcc cccttcctgc tgggtaaaga
    30901 catcgcccga ttcacataat ggcccggcga cgacggtttc caccgttggc gcgtgttcca
    30961 gagaacgacc atcagctgcc agggcactga tatggtggta actaccgtac attgccgggc
    31021 gcatcagatc gttgaacccg gcatcaacca gcacaaagtg gcggctaccc atttgtttga
    31081 cgctccgcac ctgagtaatt aacacgccag actgcgctac cagaaagcga cccggttcaa
    31141 tttccagttt cacagggtgg cccaaatggc gagcgatttg ctcacgcgcg gcattccaca
    31201 gaccataata atgttcggta tcaaccgcct cttcaccctg ttgataagga atagaaagcc
    31261 caccgcccgc agaaatagcc tgtaaatcct gaccgaattc gatgacctga cgtaccatag
    31321 caccacacac ctgttccaga tgggcataat caacgccaga accaatgtgc atgtgaatgc
    31381 cgaccagctg caaatgatga cgttgtatca cgtccagtgc ggcgggcaga tcggtgtacc
    31441 agataccgtg cttgctgttt tcgccaccgg tattggtttt ttggctatgt ccgtgaccaa
    31501 accccgggtt aacgcgcagc catacccgat gccctggcga aacctggccc agttggtcga
    31561 gcatatcaac agaacccgca ttcaccggaa tttgcaattc actgacgcgt tcaagcgtcg
    31621 cctgatcgat aacatctgcc gtaaaaacaa tatcatcggg gtgcgtttgc ggattgtaac
    31681 ccgccgccaa cgcacgctct atttcgccta acgagacgga atccactttc acgccctgct
    31741 cacgcattaa tcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag cgcaccacat
    31801 caaactgttt cagcgctgca atctgccgac gaataatttg cgcatcgtag acccacaccg
    31861 ggcagccaaa ttcagcgggc aaacgcagca gattttcggc ggtgagatcg gtatcggtgc
    31921 tgaacagtga atgtggcata acaaactcca gatgagtgct tttttatgat tacgccacat
    31981 cataaaaaga ataaaaaata tcgatttatg tcgagtctat gcaaaaatga tatggattac
    32041 cggattgcga gagagcgcta atggccgccg ttaacttacg tcatattgaa atttttcatg
    32101 cggtaatgac cgccggaagc ctgactgagg cagcacacct gctacacacc tcacagccaa
    32161 ccgtcagccg cgaactggcg cgctttgaga aggtgatcgg gctaaaattg tttgagcgcg
    32221 tacgtggacg attacatcct accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat
    32281 cctggtacgg actggatcgc attgtcagcg ccgcagaaag tctgcgcgag tttcgccagg
    32341 gagaactgtc tattgcctgc ctgccggtct tttcgcaatc ttttttaccg cagctcctgc
    32401 aaccctttct ggcacgttat cccgatgtca gcttaaatat cgtgccccag gaatcaccgc
    32461 tacttgaaga gtggctctcg gcccagcgtc atgatttagg actcactgag acactccata
    32521 cgcctgcggg aacagaacgt accgaattac tctctttaga tgaagtgtgt gtgttacctc
    32581 cgggtcatcc gctggcggta aaaaaggtat taacgccgga tgattttcag ggtgagaact
    32641 acatcagcct ttcccgtact gacagctatc gccagttgct ggatcagcta tttactgaac
    32701 atcaggttaa acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg
    32761 tacgggcggg ggtaggtgtt tcggtggtta acccgctcac cgcactggat tatgcggcaa
    32821 gcggtttagt ggtacggcgg ttcagtattg cggttccgtt caccgtcagc ctgatccgcc
    32881 ccctgcaccg cccgtcatca gcgctggttc aggcgtttag tgagcattta caagcggggt
    32941 taccgaaact ggtcacttct cttgacgcga ttttgtcgtc agctacgaca gcataaaagc
    33001 gacagcatcc tcggcatgga tcgccgcagt atcaaacaca ggcaaaacac tgcgctcttc
    33061 tggaaccaat aaaccaattt ctgtgcagcc aaaaatgaca ccctgcgccc cctgttctgc
    33121 aaggcgagca atcacttgcg cataataagc gcgtgacgct tcggtaaatt gccccagaca
    33181 cagttcttca aaaataatct gattaatttt cgcccgttca tccgcttcag gaataagaca
    33241 attgatggaa aattgttccg tcagccgccc gcgataaaaa tcctgttcca tggtgtaacg
    33301 cgtacccagc agcgccacac gagtcattcc ggccccggta attgcacgtc cggtggcatc
    33361 cgcaatgtgt aagaaaggca gagtgcaacg tgactcaatg gcatccgcca ctttatgcat
    33421 cgtattggta cataacacaa taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc
    33481 agccagaatg tccccggttt tatcccattc cccgcgacgc tggcactctt ctatttcatg
    33541 aaaatcgacg ctatgtagca gcacttgcgc agagtgaagc ccaccaagcc gctgtttaat
    33601 gccttcattt atcagacgat agtaaggaat ggtggattcc cagctcattc ctcccagcaa
    33661 accaattgtt ttcattcctc tctcctgatg tgtgttaccg caacaaggct gaataaggaa
    33721 cgagacaaac gcctcaacgg ccaggtgccc gatctctatt aacgaaaaaa gggccggatg
    33781 tacagcacat ccggcccgtg aaatcagacg ccgatatttc tcaacttctc gcctgccatc
    33841 agtttgcgtt cgatatgttc cagcgtgaca tttttggttt ccggaatgag ccagaaagtg
    33901 atgcccacaa acgcaatgtt cagcgcagtg tagagccaga acgtaccggc agcgccaatg
    33961 ctatcaagca gtgtcaggaa ggtcgcgccg ataatcatat tcgacaccca gtttgtcgtc
    34021 gtcgaacagg taataccgaa atcgcggcat ttcagcggct gaatttcaga gcacaggatc
    34081 cacaccactg gcgcggcgct catcgcataa ccggcaatac acatcatcgt catgccaaca
    34141 gagagccagg acaagccact ggaagccgta ccgttatcaa actgcatcag gcaatagccc
    34201 agcaccagag tgcctaacgc catcacgctg aaaccaattt tcagagccgg tttacgacct
    34261 gctttatcta ccgtaaagac cgcaatgaag gtcgcgaaca taaaggtcag tcccacgacc
    34321 agagtcgcaa tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc
    34381 gcatagtaca tgatgatgtt cataccggta aactgctgca tcgcctgcaa caacataccg
    34441 agaaacacag cacgacggac gttacggttg atcttaaaca gtgcccaacc gccttgtttt
    34501 aacttcaggc tttcacgaat ttcgttgagt tcttctcgcg ctttttccga cgtatcgcgc
    34561 agcatacgca acacttcttc cgcctcaata tgacgcccct tttccgccag ccagcgcggg
    34621 ctatttggca ggaagaccac cagaataatc agcaggactg ctggtaaagc aagaaccccc
    34681 aacattgcgc gccagttacc gctataactg aacgctgtat cggataaaaa cgccagcacg
    34741 atgccgagtg tgaccatcaa ctggtacata ctgatcatct taccgcgaac gttttcactt
    34801 gccatttcag aaagatacag aggagcggtg taagacgcga tcccgacagc aatgcccagc
    34861 accacacgag cggcgattaa catctctacg ctggtcgcaa aagcggaccc tatagaaccg
    34921 agtacaaaca ggatggcccc cgccatcagg ctgtatttac gacccaggcg gaacgacagc
    34981 caaccattaa acagcgcacc aattgctgcg ccgagcatca tgctactaac cacccattcc
    35041 tgcaaacgac tggtcagcac aaagtgatcg gtaatgaacg gcaacgctcc ggcgattacg
    35101 ccgatatcaa gaccaaataa caatcctgcg accgcagcag ctaccgaaac aaacatattc
    35161 atacgccgcg tatcccgcaa agaacgtggc gttaaagcag attccgtatt gatagtaacc
    35221 attttttcct gccagcagag agtaagacat agtgaaaaaa tacgtgaaca actcacgcag
    35281 gtgtcaggtc ggaaacagca taaatatgga ttaaattgct gcgacatgtc gttatgtgat
    35341 ggatattcca attttcaaat taagttgaat tattgagatt attattaacc acctaatttt
    35401 acagcagata aaattcataa agt
//
LOCUS       DAZGOB010000043        33819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGOB010000043 DAZGOB010000000
VERSION     DAZGOB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.043"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1700)
                     /locus_tag="ACHLIF_002177"
     CDS             complement(<1..1700)
                     /locus_tag="ACHLIF_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9606189.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQNRPSDKAWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPEGVAEYSVWGLKLPTL
                     R"
     gene            complement(1761..2408)
                     /locus_tag="ACHLIF_002178"
     CDS             complement(1761..2408)
                     /locus_tag="ACHLIF_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9606190.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(2306..3049)
                     /locus_tag="ACHLIF_002179"
     CDS             complement(2306..3049)
                     /locus_tag="ACHLIF_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9606191.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVALSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            complement(3054..3752)
                     /locus_tag="ACHLIF_002180"
     CDS             complement(3054..3752)
                     /locus_tag="ACHLIF_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9606192.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(3762..4091)
                     /locus_tag="ACHLIF_002181"
     CDS             complement(3762..4091)
                     /locus_tag="ACHLIF_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9606193.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCDHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(4091..7156)
                     /locus_tag="ACHLIF_002182"
     CDS             complement(4091..7156)
                     /locus_tag="ACHLIF_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9606194.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARVAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            complement(7128..7457)
                     /locus_tag="ACHLIF_002183"
     CDS             complement(7128..7457)
                     /locus_tag="ACHLIF_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9606195.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            complement(7466..7852)
                     /locus_tag="ACHLIF_002184"
     CDS             complement(7466..7852)
                     /locus_tag="ACHLIF_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9606196.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            complement(7913..8656)
                     /locus_tag="ACHLIF_002185"
     CDS             complement(7913..8656)
                     /locus_tag="ACHLIF_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9606197.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            complement(8667..9068)
                     /locus_tag="ACHLIF_002186"
     CDS             complement(8667..9068)
                     /locus_tag="ACHLIF_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9606198.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            complement(9065..9643)
                     /locus_tag="ACHLIF_002187"
     CDS             complement(9065..9643)
                     /locus_tag="ACHLIF_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9606199.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTVRIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGSLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(9655..9930)
                     /locus_tag="ACHLIF_002188"
     CDS             complement(9655..9930)
                     /locus_tag="ACHLIF_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO9606200.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMIVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            complement(9923..10246)
                     /locus_tag="ACHLIF_002189"
     CDS             complement(9923..10246)
                     /locus_tag="ACHLIF_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO9606201.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSEPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            complement(10333..12360)
                     /locus_tag="ACHLIF_002190"
     CDS             complement(10333..12360)
                     /locus_tag="ACHLIF_002190"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO9606202.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(12305..13813)
                     /locus_tag="ACHLIF_002191"
     CDS             complement(12305..13813)
                     /locus_tag="ACHLIF_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9606203.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            complement(13813..14025)
                     /locus_tag="ACHLIF_002192"
     CDS             complement(13813..14025)
                     /locus_tag="ACHLIF_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606204.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(14022..16121)
                     /locus_tag="ACHLIF_002193"
     CDS             complement(14022..16121)
                     /locus_tag="ACHLIF_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9606205.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            complement(16130..16657)
                     /locus_tag="ACHLIF_002194"
     CDS             complement(16130..16657)
                     /locus_tag="ACHLIF_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO9606206.1"
                     /translation="MSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPVAATV
                     RGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQRKLI
                     PVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQEKSYE
                     ACAAELIPDEEGESL"
     gene            complement(17230..17436)
                     /locus_tag="ACHLIF_002195"
     CDS             complement(17230..17436)
                     /locus_tag="ACHLIF_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606207.1"
                     /translation="MNKEQSADEVSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            17737..18147
                     /locus_tag="ACHLIF_002196"
     CDS             17737..18147
                     /locus_tag="ACHLIF_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO9606208.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFR
                     WVTNIQEHQRYYYAFDNSLLFTESIQKTTQILPR"
     gene            complement(18299..18472)
                     /gene="gnsB"
                     /locus_tag="ACHLIF_002197"
     CDS             complement(18299..18472)
                     /gene="gnsB"
                     /locus_tag="ACHLIF_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO9606209.1"
                     /translation="MNIENLKTKAEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDVKINLI"
     gene            complement(18644..18874)
                     /gene="ynfN"
                     /locus_tag="ACHLIF_002198"
     CDS             complement(18644..18874)
                     /gene="ynfN"
                     /locus_tag="ACHLIF_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFO9606210.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(18879..18944)
                     /gene="ynfR"
                     /locus_tag="ACHLIF_002199"
     CDS             complement(18879..18944)
                     /gene="ynfR"
                     /locus_tag="ACHLIF_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFO9606211.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
     gene            complement(18947..19135)
                     /gene="ynfQ"
                     /locus_tag="ACHLIF_002200"
     CDS             complement(18947..19135)
                     /gene="ynfQ"
                     /locus_tag="ACHLIF_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO9606212.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(19146..19358)
                     /gene="cspI"
                     /locus_tag="ACHLIF_002201"
     CDS             complement(19146..19358)
                     /gene="cspI"
                     /locus_tag="ACHLIF_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFO9606213.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
     gene            complement(19721..20218)
                     /gene="rzpQ"
                     /locus_tag="ACHLIF_002202"
     CDS             complement(19721..20218)
                     /gene="rzpQ"
                     /locus_tag="ACHLIF_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO9606214.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
     gene            complement(20215..20748)
                     /gene="rrrQ"
                     /locus_tag="ACHLIF_002203"
     CDS             complement(20215..20748)
                     /gene="rrrQ"
                     /locus_tag="ACHLIF_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9606215.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVESNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            complement(20745..21056)
                     /gene="ydfR"
                     /locus_tag="ACHLIF_002204"
     CDS             complement(20745..21056)
                     /gene="ydfR"
                     /locus_tag="ACHLIF_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO9606216.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            complement(21061..21276)
                     /gene="essD"
                     /locus_tag="ACHLIF_002205"
     CDS             complement(21061..21276)
                     /gene="essD"
                     /locus_tag="ACHLIF_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO9606217.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            21496..21666
                     /gene="ynfU"
                     /locus_tag="ACHLIF_002206"
     CDS             21496..21666
                     /gene="ynfU"
                     /locus_tag="ACHLIF_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFO9606218.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
     gene            complement(22030..22245)
                     /gene="cspB"
                     /locus_tag="ACHLIF_002207"
     CDS             complement(22030..22245)
                     /gene="cspB"
                     /locus_tag="ACHLIF_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFO9606219.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIERGAKGPAAANVIITD"
     gene            22546..22758
                     /gene="cspF"
                     /locus_tag="ACHLIF_002208"
     CDS             22546..22758
                     /gene="cspF"
                     /locus_tag="ACHLIF_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFO9606220.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            22813..22902
                     /locus_tag="ACHLIF_002209"
     CDS             22813..22902
                     /locus_tag="ACHLIF_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606221.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(23180..23932)
                     /gene="ydfT"
                     /locus_tag="ACHLIF_002210"
     CDS             complement(23180..23932)
                     /gene="ydfT"
                     /locus_tag="ACHLIF_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9606222.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(23946..24995)
                     /gene="ydfU"
                     /locus_tag="ACHLIF_002211"
     CDS             complement(23946..24995)
                     /gene="ydfU"
                     /locus_tag="ACHLIF_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9606223.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            complement(24997..25275)
                     /locus_tag="ACHLIF_002212"
     CDS             complement(24997..25275)
                     /locus_tag="ACHLIF_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606224.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            complement(25342..25593)
                     /gene="rem"
                     /locus_tag="ACHLIF_002213"
     CDS             complement(25342..25593)
                     /gene="rem"
                     /locus_tag="ACHLIF_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFO9606225.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            complement(25810..25965)
                     /gene="hokD"
                     /locus_tag="ACHLIF_002214"
     CDS             complement(25810..25965)
                     /gene="hokD"
                     /locus_tag="ACHLIF_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO9606226.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(26037..26324)
                     /gene="relE"
                     /locus_tag="ACHLIF_002215"
     CDS             complement(26037..26324)
                     /gene="relE"
                     /locus_tag="ACHLIF_002215"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFO9606227.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            complement(26324..26563)
                     /gene="relB"
                     /locus_tag="ACHLIF_002216"
     CDS             complement(26324..26563)
                     /gene="relB"
                     /locus_tag="ACHLIF_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFO9606228.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            26588..26893
                     /gene="ydfV"
                     /locus_tag="ACHLIF_002217"
     CDS             26588..26893
                     /gene="ydfV"
                     /locus_tag="ACHLIF_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFO9606229.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            27096..27428
                     /gene="flxA"
                     /locus_tag="ACHLIF_002218"
     CDS             27096..27428
                     /gene="flxA"
                     /locus_tag="ACHLIF_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO9606230.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLRAQLEQLLRQQAEKKNKDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(27865..28014)
                     /gene="ydfW"
                     /locus_tag="ACHLIF_002219"
     CDS             complement(27865..28014)
                     /gene="ydfW"
                     /locus_tag="ACHLIF_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HFO9606231.1"
                     /translation="MDNARIDLRSKYYVKSKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKID"
     gene            complement(28087..29205)
                     /locus_tag="ACHLIF_002220"
                     /pseudo
     CDS             complement(28087..29205)
                     /locus_tag="ACHLIF_002220"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1202-like element ISEsa1 family transposase"
     gene            complement(29239..29658)
                     /locus_tag="ACHLIF_002221"
     CDS             complement(29239..29658)
                     /locus_tag="ACHLIF_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9606232.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKARRSRLLLTPSCNVPFEPF"
     gene            complement(29699..30664)
                     /locus_tag="ACHLIF_002222"
     CDS             complement(29699..30664)
                     /locus_tag="ACHLIF_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606233.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGLKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDQQTAEQDFLTRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDQFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            complement(30645..31166)
                     /locus_tag="ACHLIF_002223"
     CDS             complement(30645..31166)
                     /locus_tag="ACHLIF_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9606234.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(31150..31377)
                     /locus_tag="ACHLIF_002224"
     CDS             complement(31150..31377)
                     /locus_tag="ACHLIF_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9606235.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            31455..31862
                     /locus_tag="ACHLIF_002225"
     CDS             31455..31862
                     /locus_tag="ACHLIF_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9606236.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            32055..32210
                     /gene="ydfA"
                     /locus_tag="ACHLIF_002226"
     CDS             32055..32210
                     /gene="ydfA"
                     /locus_tag="ACHLIF_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9606237.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            32212..32313
                     /gene="ydfB"
                     /locus_tag="ACHLIF_002227"
                     /pseudo
     CDS             32212..32313
                     /gene="ydfB"
                     /locus_tag="ACHLIF_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            33274..33462
                     /gene="dicB"
                     /locus_tag="ACHLIF_002228"
                     /gene_synonym="ftsT"
     CDS             33274..33462
                     /gene="dicB"
                     /locus_tag="ACHLIF_002228"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO9606238.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            33459..33650
                     /gene="ydfD"
                     /locus_tag="ACHLIF_002229"
     CDS             33459..33650
                     /gene="ydfD"
                     /locus_tag="ACHLIF_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFO9606239.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            33743..>33819
                     /locus_tag="ACHLIF_002230"
     CDS             33743..>33819
                     /locus_tag="ACHLIF_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9606240.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVAT"
BASE COUNT         8445 a         9255 c         7412 g         8707 t
ORIGIN      
        1 cgcagcgtcg gcagcttcag cccccatacg ctgtattcag caacaccctc aggaacacgg
       61 ctcactttta ccttcacgcc gtcggtgacg gactgaacct ccacgctgac cggattgcca
      121 cttccgtcaa ccaggcttat cagcgtggta ccggaggatg gcagcgtgat ttcacggtcg
      181 agcgtcagcg tccgggtctg gctgttcacc gccagcacac gaccaccggt gctgataccg
      241 gcatagtcat catcgcagat ttcaataaca tcgcccggta catggcgaag cccttctgcg
      301 ccgacgctga aatccacggt ctgcgtctcc agcagctccg ttttaatcag ccacagcccg
      361 gcgcggtgtg cctgcccccg gctggtacag ccaaaggcat ccatcttcgt aacattacga
      421 ccgtaacggg caatggcctg cgtatcttca acaagctctg tcgccgtctc ccagccgttg
      481 ttcgggtcaa tccagttcac ctcaacggca ttatggcggt ctttcagggc gctgaagctg
      541 tagcggaacg gcgcgccatc atccggcatc accacattac tgcggttata ggtccacgcc
      601 ttatccgacg gtcggttctg cacgaacgtc agcgtctgcc cgttccatac cggcatacag
      661 cgcatcgccg agcagaaatc actgagaacg tcccacgcct tgcgctgtgt ggtcaggtac
      721 gcattacagg tgatgcgcgg ctccgtgccg ccaaagccgt ccggcactga ctggtcgcaa
      781 ttctggccga tgacatacag cgcccattta tccacatccg ccgcaccaag acgtttcccc
      841 atgccgtagc gcggatgggt aagcatatcc cacagacacc aggccatgtt gttgctgtat
      901 gccggtttta acgttccgtc ccagataccg ctgtattgtc gcgtctgcgg gttataattc
      961 gacggcacct gcagaatacg cccgcgcaga tgataattac ggctcacctg ctggctgccg
     1021 aactgctccg aatccacctg tacgccgacc agtgccgtgt tcgggtagca ctgtttcaca
     1081 tcgatgattt cggtgtatga cgaccagagc gttttgttct gcagctggtc tgtggtgctg
     1141 tccggcgtca tcctgcgcat ccggatactg aacgggcgcg gcggcaggtt atccaccacc
     1201 acagaggcca gatactgcga ggtggttttg cctttaatgg tgatgtcttt ttccgtcacc
     1261 cagccaccgt tacgttgtat ctgaaccagc aggcggactt ccgatggatt cctgtccccc
     1321 tttgaggtgg tttccaccag tgcctgcaca ccgaaggtaa agcgcagacg gtcgatgttt
     1381 gccgacgtga tggtccgggt gatcggcgtg tcgtatttca cttccgtccc cagcaccgtc
     1441 tcggatccgg aggattcaaa tccctccggc ggtgtctgct cctgctcacc ggcccggaac
     1501 accaccgtga cgccggagat gttggtattc ccctcagtgt ccagcactgg cgtactgttc
     1561 agcagcacgc tttttaatcc atccaccgga ccttcaatcg gcccttcgct gatggcatcg
     1621 atcacactca gcaactgcgt ggacttcagg ttgtccttcg cttcgcgcgg agtatgcccc
     1681 ttactgctgc ctttacccat tcctaacgct ccataaacga caaaaccgcc cggaggcggt
     1741 ttcacataaa acgtttttca tcagcgacca atcactacaa cctgaccacc atccccttcg
     1801 tctgccgtgc tgatctcctg agagaccacc cgcgacccca cgcgcatttc accgtacaga
     1861 acgggcagaa cattgccctg ggcaaccatg ttatccagtg aggagaaata ggtgttctgt
     1921 ttgccgttat ctgtacttgc tgccgtgggc gtcctggctt tcggtgccag catctgcgcc
     1981 actccgccca ggatcatact ggcccctgcc gcatacatgc ccgatacagc cgcggcaccc
     2041 agccagccca cagggttcca ccatgccacc gcaatcagcg ccgcccccag caccacctga
     2101 aacacaccgc cacttttagc tcccgccaga cgcggcacga tgtggatcac ggcaccattt
     2161 gccagcggct cattaagacg ggcagacaat tcggtttcac ctgcatcacg cccggcaatg
     2221 cgtacctggt accagccgtc gctcagtttc tgacgaaacg ccgggatctg catggccagc
     2281 gcccggatgg cttcagaccc cgttttcact cgaaggtcga tgcggcggcc aaatcgttgt
     2341 aaatccccgt aaaggcagat gcgtgccatg cccggtgacg ccagagggag tgtgtgcgtc
     2401 gctgccattt gtcggtatac ctctctcgtt tactcagttg ttcaggaata tggtgcagca
     2461 gctcaccgtc gccgcagtaa atggcggcat gattcggcac cgatgaacca aaacagcaca
     2521 gcagcacatc gcccggttgt gctgatgaca acgccacctg atacagccct gtggcctcca
     2581 gattatccag atagagattc tgaccgttac gccaccagtc atccccgcga tgaaaatccg
     2641 gcatctcaat ccccgccaga tgataagcat cccggaacag cgtgtaacag tccgtcaccc
     2701 cgtgctcaaa gcgccgcccg gtgagatgcg gcacacagcg gaacttgtga atcgcccccc
     2761 ggcagaccag ccaccacggc aaatcactct gcacctgcag ccgccggtca gcctcactca
     2821 gccagggcag accaccggga tgactgtgga ccagtgccac aatctcaccc tgcatctctg
     2881 cctgcagcca gtcctccgga gccatccgga aataatcctc cggctcaccg gaaatattca
     2941 cgcagggaaa atatctttcc ccctccggcg ctctcaccac gaagccgcac gactccgctg
     3001 gcgcacatcg ccgggcgtgc gccagaatcg ctgattctgt ctctgtcatg ggattactgc
     3061 gaaagtttgt taatggaaag gaagccgcca aagttgccga cgttattgcg aaacttacag
     3121 ccgctcaggc atttgctgca tttatccttc gtgatatcgg acgtcggctg gtcatattca
     3181 tccgcgactg ccggaccgtg ataaccgcac tcatcaccgc gataggtcca ggtgcaggtg
     3241 ttggccagca tgatacgtcc cggaaaaaca gcgccgtccg tttccgtcgg cgtggacagt
     3301 acaaaagagg cactcaccgc gctcagttcg ctgcactgct caatgcgcca gcggctgatc
     3361 acctcctgct ccggatcggc gtcactgttt ccgttgacga agttcaccgc atccagaaaa
     3421 cgggcgtaaa ccttacgccg gaccaccgtt ccgccgacca gactctgcag atcttccgcc
     3481 atcccggtga ccataccgta caggttagaa accgtcagtg tcggacgggc agcactgccc
     3541 ttgccgttca gttcaaatcc cgtcccctga atggggtata cctgatactg ccgcccctgc
     3601 cagataaccg gctcaccttt ttcgttctgc tcattacaga aaaaataacg ttcaccaccg
     3661 acctctgtca gatcgatttc ccagagcacc acctgggctg actgagtgag gcgtgtcgtc
     3721 tcatgatgtg tttcctgtgg aatatcctgc atcagagcct cctatgccac gacctgttca
     3781 aaatctgccg ttatggttac ccacagcgcc cccacgcttg ccgaccattt acgacaaacc
     3841 accctgatcg gtttccagtc ataaggtggc gtccactgaa atgcacggac gccaccgtgc
     3901 cgttccagaa aggcttttaa agatgggtgg tcacatttac gaacacgtat cgtcacgctg
     3961 taagtcgaca actggttatt cagtcccgcc gcacgacgct gttcataacc atcgcccagc
     4021 ttcactgtca ccactttcgg ctctgatacc acattcatat ccgggcgcac tttccagtga
     4081 aacgtctcca ttaccgatat gctccactta accgaccacc atcacgggcc tgctgttgca
     4141 taaagtccgc tgccgctttt ttcccgaggt cataaaccgc cttcagggca gccggaccta
     4201 tctgcccgtt cgtgccatcg ttattgatct cgatgttgta ctgcggggaa aacatcgcca
     4261 tacctgaccc cccaatatcc gccacaaccc ccagcttacc gtcagcacca cgacgcaatg
     4321 gcagaatggc ttcaggtccc gcttccccca tcacacccgc gccttttgca aaagcaaaaa
     4381 acgtcggacg gttaaccacc gtgccactgt aacgactcaa atcagcagac tgataaatcc
     4441 ccccattggc attcagggaa aggctgctga gatcaaatcc cagtacactg ccaatccctt
     4501 ttatcgattt catcatggtt gcctgcgcca ggattttcgc catatctgac agcacagaag
     4561 aggtgaagga tttgaaattg agtttgccgg tagtgacaaa agttgccagg ccatttccca
     4621 tgttgctgaa ggctgacgag aacacctgtt ctgctgtttc tgccgtatta tctgcatcca
     4681 cggtaaaatc ctgaaatgca cgcaggactc cgtttttcca gttaccctgc acaacttcaa
     4741 gctgttgcca gtaacggcga ttctcattca gttgtcggtt caggctctcc gtcagcgcct
     4801 gctcggcctt tctgtagtca tccgtgttat atgtcccttt ctgctcacta tcccgcctca
     4861 actgctccag ctgttgctgg tatttctggc gaagactcag ttgtacctga tatcgctgtc
     4921 gctgctgatc acccataccc accgtggcga tatccaggtc atgttgctga cgctgagcgc
     4981 gctcttcttc agccagttga ctggtcagct gaattgtttt tttcttcagc tcgttgagtg
     5041 ccgtctgttt ctgaagctcc tgctgtttta catccagcag cgtcagtgcc tgaatcagtt
     5101 catctttacg ggccagcaca ctcttttcat ctgccgtcag ttttttcccg tccaggtcgc
     5161 tgatgcgctg ctgcagagcc agaagctgtt tatgcgcttc tgtcatcctt tccgtggcaa
     5221 tgcctgctga ctgtctggca gcagcaatct gtccttccac ctgtgcctgt tgctgactgt
     5281 actgcagcaa taaccgggtg gcctcatcat tacgggtttc gcgtgttttt ttcttaccgg
     5341 atgccagggc tttctcgtaa cgttcatttt cacgttgtat cgccgcatcc ctgacagcct
     5401 gatcggcgta ctgcatggca ttaatacgcg caatttcacg ctgatgtcgt gctgcttccg
     5461 tttcattcat ccggttcagt gcagcatttt cagcattacg gcgtttctgt tgctcctgat
     5521 aattccgctc tgcctgctct tttgcatcct gcaaatcctt ctggcgtttt ttctcctgaa
     5581 gatcgttaag acgctgctga tcgtattcaa cctgagaaga tgatgccgtc caggggagtc
     5641 ttttcgcccg cgacactttc tcctgtaaag cggcaatctg ttcatccagc gagtcttcac
     5701 gaccaatatt catggccgca tcccagaaac gactccacaa atcagacaga tacttcagcg
     5761 tactgccaag cgcattgagg ttattatcaa tatccgcagt acgccgaccg gtttcctctg
     5821 ccagtgcaga catggctatc cgtgcagcat cactggaccg cccctgctcc ccaaggacgc
     5881 gtatctgctc aagctgagtg gcagtaagaa aatgcagctc attgtccaga gccttcgcgg
     5941 catttacagg atcatccttc agccgcttaa actgatttat ggtatcgctg accgactggc
     6001 caaccgatcg ctccatctgt gcggcaactc tcgccaccat accgatatcg tttccacgaa
     6061 atgcaccact ccccaccacc tgagccagcg caccggctgc agcatgttgc gtgataccat
     6121 tcccggaaat agcacgactg agcgtccaca gctgcccggc agtgactccg gcataatgcc
     6181 ccgtcagcga caactggcgg ttaaattctt ccccctcctt ctgaccgtca taccaggctt
     6241 tacccagacc atagacggcc gcggcaatac cgccaataac cccgccaagc atcatgcctt
     6301 tcggtgacat cagtgtgtct atccatccgg cacggttagc cagcgttatc ccggatcccc
     6361 tcagcgcccc taaattgccg cgggccagtt cacctatcag aacgcctatc tcctggcggg
     6421 ctgctgcact tttcagaccc agcgaatgcg tggcttttcc tgcctgctcc attttgcgga
     6481 tatacacttc tgcagcactg cttaccccca gctgggctgc cttagcacga agcaactcag
     6541 aagatgaaag attcaggcgg gttgcctgct ctttaagctg acggataaac gccactttct
     6601 gtcgggtagc ctcttcctca gcctgtgtaa gaacacgggt tttcgcagta acctcagaaa
     6661 tcagcgccag ataatcctgc tgaccaatcc cgccactgtt tctggcctgt cggatctgct
     6721 gctgaatacg ctgtaattcc tgcagccccg cactggcctg tttcacactg tcaatctgac
     6781 gataaaacgc agcagccgct ttatcctgag cctccgccag agccatggcc tgcgcctgtt
     6841 cctcgcgcat tttctggctc agtgcctcca tgcgctggcg ggttttctcc acctcgcggg
     6901 ccatgcgttc atgagcctgt gcgttcttct ccaccgtctg cgcatggacg gatgcggctg
     6961 ttgcagccga agaagctgcc tgcgttgtct gccgggcggc ctgagtctga cgctccataa
     7021 aacgctgcat acgggcagaa gaccgttctg catcgctggc tgcaccattc agaaggtttt
     7081 tgatacgggg aatttcattt ttaaactctg ccgcatcaat ccccaaatca atgaccaggt
     7141 tggctatctg gtccataacg cacacctccg gaaatacctt ccccaagatg catcagttct
     7201 tcgtccgttc gctccggtat cccgttctct tccggtaaaa ggctgaaatc agccaccgca
     7261 gcatcactgc tgccggacac cattctcacg atcaatgcct tcagcgaggc aaactgcgca
     7321 tccatccaca catcactgaa gctctgcatc cggaaataat cgccccactc accaagctca
     7381 gtggccgaca tttccgacag catccgccgc cagtctgccc gccggaactc ccgggcaagc
     7441 cgcatgacaa actgcatttc ccgcgtcagg acttttccgg cgtcagcgcc tcatgctcat
     7501 catccacggc attatcaatg gcccccatac cgctcagcga cagaaccatc tccgcccccg
     7561 cacccagggc atcatacgac catgttgtaa taacggatgc gcaaagcgtc tctacatcct
     7621 tagactgatc cgcattccac agtgagcggg aaaccagcca ggcattgata tccatcccca
     7681 tccgcagaaa agcaatctgt cgttcagcct ccggcagttc tccctccccg gcatcaaact
     7741 ttgccgttcg ctgctgaaca aacgtcagat attcaattct ctgcagcccg gacagctcac
     7801 tgagcaccac ggactgtttt tcataattaa acgtgtcctg tttcagaaac atcatgttct
     7861 ccacctgcaa aaaagccccg aataaccggg gcaaatgatg agtatcgtcc tgttaacctg
     7921 ctgcgctgac agtcaccgca gtcacagcca caaaattccc gtcagcggtc atgcccacaa
     7981 tgctgacact gccctgcttc acgcctttta ccgtggccac aagtccgttc agggtaaccg
     8041 tggcagtctg tggatctgac gaatgcacac tgatcgcttt gtcactggct ccgtcaggtt
     8101 ttactgtaaa ggtcagcgtg gtggttgctc ccacttttac actggcagac gccggtgata
     8161 ctgtcagccc ggtaacgctc acggtttcag tgccttcctc tgccagatac ggacgcccca
     8221 caccgctgat tttcacagtg cgggtcatca cgtcttttga ggcaatggtt ttacccagtg
     8281 agctcagcca gccacggaaa acatcaacag tgccgttggg atatttgata cgaaacgcgc
     8341 agacttcacc ggagtcgaac aactgaacca gttttttctg cccgctgtca cccggacgcc
     8401 aggccagcgt cgccgaagta tcaccaacgg atttctgccc ctgggttgtc gttttccagt
     8461 ctgcatcttc atcatcgaga taagtgtcat cttctgcatc agcggtcatt tcgccaggtt
     8521 gcagatcctt caccatcgca agacgcagcc agtcagtgtc cgacaaaggg ttcgcaaacg
     8581 catcgccgtt gccggtatac atccagaacg tcgtccccgc acctttcgtc tttgccagtg
     8641 gatttggtgt ggtcattacc acctccttaa ttcgtgtacg tgatctggta cgtgatttcc
     8701 gccatcgccc aggtggccat ctcattatca cgttgatagt taaaaccgag tgggatcagg
     8761 gtgtcgatga gtccggaaag tgccggtata tcattcaggg cagggaaaat ggtgctctcc
     8821 atccacatat ccagctctga atccggtgcc tgtgcccgga tgaagacggc aatatgcaga
     8881 acagcctgcc agtcatcttc atccgtcatt tttccggtgt actgagcatc actcagccac
     8941 accgccacgg caggcagttc ctgcgcatca ataaatgccg gaagcccgtc aaaaaacgtg
     9001 gcgctgtctc cacactgttc ccgaaggcgt gccagtacgg cctggcggat ttgtgtatgt
     9061 cggttcatcg ggtcagccat aacctcagtt gttgttttag tgcatacccc agctgtttcg
     9121 gcatttccgc agcaatgatg tggtcgcggg catcttcaaa tgcctgtgtc agcgatccgg
     9181 acagcgggat tttcaccaca tcaatggggt aacgattttt gccatcaata cgtcgcatca
     9241 catgccagcg accattcgcc agttgctgaa taaacgcatc ccggaaaaga tatttaccca
     9301 ccttcagcac actgccacgg tactgcagtt ttccaccacg ccgggccagt ctgacccggg
     9361 ctgtccccag cttaatggcg ggcagattgc cccggttaac gcggatcctg accgtcattt
     9421 ttcctgacgg actggcttta aacacccgga cacgctgacg taccagtttc agggggatcc
     9481 ctttcacctg gttatctccc gcaacggtat tcccggcaac ctgccgggtg gcaaccgaga
     9541 ccgctttctg tgccacacgg tttatcgccc atgcgctggc ctgtggcacc atacgggtat
     9601 caaggctgtt cagattgcgg atggcattct caagcccctt catcccacac ctctttactc
     9661 aataaagatc attggcttac cgttaaaacg ttcatgccgt gtgaccgtcc attgttgtcc
     9721 gtcataaaca acgcgatccc cgcgccgtgg gcggtatccc gaagaaaaca ccaccagaga
     9781 gaccgcaggt ccggacagag cattcagctc tgccagtgtt tctcccggga tcacaatcat
     9841 atcgacatca ttaatcgagg ctgtctttcc catctttctg accgtgatcg catccatacg
     9901 cgctgccagc cgggaaaagg gatcagacat tgagttttac cggcacttct tctgcactgg
     9961 ttccggcatc tgcccagaca accccgacca gcggctcaga gccgctgtta gtcagctgaa
    10021 cttttcctga cttcagataa accttcttac ccgttttcat gtcatccgtt ttcagcttag
    10081 gcagcataaa cacaccttcg gttatgccgt cgcctgtttc tccctgtgga atatcggtca
    10141 gcgccaccgc aaaaacatcc cccacctgca ccagatctcc gctgctgatg gctgcactgg
    10201 caacaatcgc caccgttttt ccttcttcta caaaattctt tgccataact gtctccgcac
    10261 agccccgttc aggggctgat ttcaggtaca aaaaaagccc ttacgggcca tcagagttgt
    10321 tgtctgcgac gtttacgccg tacatttcac caggccgcgg tgatcaactg gcgcaacacc
    10381 agcgtcaata cgcactttcg ttgtcacgcc atccacactg aagccctcca tctgatcaat
    10441 atatggcgta tccacaccgt tgagataagc cacttcaatc gtatcggagc ctttggacgc
    10501 agccaggtag aaggtggtct ggctgttatc atcaagacga ggctctgcaa taacggtcgc
    10561 aaaatctttc accgggttaa taataccggc gttaatgtca gcccccttga cacttgagga
    10621 gcgaatgacc tggttagcaa cagactccat cgccgtcggt accagtacga acgcaggacg
    10681 aatattcaga tgacgctcac cctctttctg aacgcgcatc aactggcggg ctttatccag
    10741 cgatgccacg tccattgccg cgctctccag tacgtttgca tgtttcgctt tatcgaacag
    10801 acttacatta tctgtggaga ttttcgggtt agacgtcaga atggcataaa ccagatcggc
    10861 aatagtggat ttcgccgcac ggcccagctt catcgggaca tcggtcagca tattcagatc
    10921 atcattgata atggcctgac gggtgatact gaacagctcg ccataggtcg ccagtgcaat
    10981 agtggcctgt ttatctccgg tggtgacgta tttatattct gccccttcac gcacctgacg
    11041 cagagcactg aagcccccca tacccacacg atgggcaatt ttaaaatcag acaactgacc
    11101 tttccgcgtc cactgttcat aggtttcagg ggcatcttcc cagccctgca gaatggcttt
    11161 gttcgcaaca tccagcagaa tattaccgaa gtcagacgta ctgtgtgtga acgccgcacc
    11221 gaccatctgc atcgggttat aactggaaac accaatacca cgttcagtca gtgacatacg
    11281 ggcatattca cgcagggtca tcccgttgta gacattatca cgttcagttt tttcaaaccc
    11341 ggcgcgcgcc atcagcgcct ggcggatccc gtcccccaca aaattaccgt taccggcata
    11401 aatatgagcc ggggtatttt tattggatgg cgtggactcg cgccccatct cgttcaacag
    11461 cttttcgcgg gcctgctcca gcgaacattc aggatcggca agacactgag cctgcaacga
    11521 ctgataacgc ccgccaaaca tggcaaacag atcattaata ccgtttacac gcgctttttg
    11581 ctctgccagg acctgcgcac ggatgctgtt ttcatccacc acgggtgctg ctgcctgcac
    11641 tggcgtccgg gaggctgcag gtccatcatc ctgtacgcgt ggagcactgt tgcgtggcgg
    11701 agtaatcata tttcgaatgg attccggcat ctttttaaat tcctctgtac gttttgactg
    11761 aatacatgcc attgccttaa cggctggtgt cacctgatca gcaaatccct gtgccagaca
    11821 ttcggcaccg gacatccagg tctcatccgc cagcatggca gcaatttcat cggtggtttt
    11881 cccggttttc tgtgcataag cgggtaacag aaccgcctca actttatcga gcaggtcggc
    11941 ataggtgcgc atgtcctccg catcaccgcc cgtaaagcca aatggtttat gaatcatcat
    12001 gaaggagttt tccggcataa tgaccgggtt tcctaccatc gcaatgaccg acgccattga
    12061 cgccgccaca ccgtcgacat aaacggtaat ggacgcacca tgtgttttca gcgcattaaa
    12121 aatggcgatg ccttcaaaga catcgccacc cggtgaatta atatggagat taatgtgggt
    12181 gatatcgccc agtgcattca gatcactgat aaactgcttc gctgtaacac cccagaaacc
    12241 aatctcgtca taaatataaa tatccgcgtc actctggtga ccagcctgca tcctgaacca
    12301 ggaattattc ttcggactgg tcgtcggtgt gctgcggctc ctgtcgtttc gttgcggcac
    12361 tactgcctcc tttatcactg gccggatcgg tatcaaatac cagatccagc ttgcggtttt
    12421 catcaatttc ggccttgcgc cgacgtttga catcatccgg attacgacca ccagcacgta
    12481 cccagtctga ttctgtcgcc gctccaccac gaatctggat tttccaggcc tcagcctcct
    12541 taacagggtc aatccacggc atcactggtc cggaatacac cgcggtatac agtgaagaac
    12601 ggtcaagatc gcggggtagc ctgataacac cggatgccac agcctgtttc agccatgcac
    12661 gatacatcgg gcgggtgacg gcaccaataa accagtcctg caggatcagg tagccatcag
    12721 tggattcaac cagctcctga cgctgggcgc tgtaagtgcc gttatagttg cgcgctgtac
    12781 tggaaaaact cagacgactg cccgccgcca cggcacgcaa ctgaccatta cgaaaagttt
    12841 caaggttagg attggggcga tccgacttca ccattccgat ttcttcgccg ggtttcagat
    12901 cgtcgtaaat aatgcccggc tgaatggtaa gctcacgttc attctccttt ctgccattac
    12961 catccggttc atagctctgc ccgtcgcctt tccggatgta catccccaga gcagcggcga
    13021 tccttgctgc agtcagctca gaatcttcat attctttcag ggcactgagg cggatcagca
    13081 caccggacaa caaagacgtc ccgcgcatct ggtgcagacg gcgaacaaat ttaagatgca
    13141 gcattcgctc tgcatccact tctttggttt ccatctgccg tccggatacg ggacggcttt
    13201 tatacaccag atatttttcg ggacgccccc agtcatcaac aaacacgccc tgattcagcc
    13261 tgttgctctc atcactagtc atgggaataa agtccggctc gagcgcctcc agccagaaat
    13321 gaacaccggc agaaggcgcc aggctgttta tgcgcccgga aaccatctgg gcaaacacct
    13381 caccatcgcg cagccaggta cgaagcatca gacgttccag catcggacgg gtaaactgcc
    13441 cggtgacttc cgggctgaca gaccattcac tccatcgggt gcgaatctcc gcagccaggt
    13501 cacgggcaat ggccccattg cgtaataccg gatgtggctc gacaataatc ccgtttttcc
    13561 ccaccacccg ttcttccagc ttgtcaaata caccaataac cagatcgtgg ttgttatcaa
    13621 ggtaacgggc ctgctcacgt aacgacacgg ccccgtactg gcttaactgg tcggcagttc
    13681 ggttctcccg tcgggctttg tgtgtccgcg tcgtttttac ggcctcataa gcctggatca
    13741 ccgcacggga acgcagcctt gccgctttcc atcctggtga aaaaacgcca atcacatcat
    13801 caagaattgc catcagaacc tcgccagccg gtacccggga tgcccccgtc gtcgtgtaat
    13861 cagagccgca aggcggcgct cccactcctg ccgtccctgc cggatctcag ataagttttc
    13921 catggtcatc tgctgaccat taaaggtgac ggattttccg tccagcaccg ccatttcagc
    13981 ttccgtataa cgctgaatca tggcttcgat atcattctgg ttcataacca tcctccggaa
    14041 gtcagccagg ggttaacatc gtcagttact gttttcttcc gtttttgttt tttaacaggc
    14101 gtggataccg gttccggtga ggatgacggt tcggtactgt cctggacaca ctccagccag
    14161 gtctcccggc tcgcccactc cggtgcatcc ggccagcgga tcttttcgta tccatgcaga
    14221 atgaccagag cctcggcata caccatcagg tcaaaagctt cgttggcacc gcgacccggc
    14281 ttactccatt tcccgtcact gctccgctct tcatacgtca gttcgtcgta aaaccagctc
    14341 cccagccagt cagggaaatg cacatagcct ggacctggcg agtcacgcca taacgcgtta
    14401 ttcacccggt ctttcagggc atccgtctga agaagccaga gcggcacatc acctgcggcc
    14461 tgcgcccggc ggcccgttcg tccggtgtta tcagggaatg tacggctgat cagttttgcg
    14521 cgccggatgc tgtcaccctt aaacaggtaa atacgtttac caaggccatc acgacggcaa
    14581 cgacgccaga atttataggc attatcagtg accccgtctt caccgccgga gtccaccgcc
    14641 attgccatca gtcgcatttg ttgagaagga tcggaggcca gcggccagct tttatgaaaa
    14701 acatccgtca gcaggacatc ccagtcttcc ggatagctgg ccggatcaat tcgctggctc
    14761 tccccgtcgc agtcactgcg caatgactgc gtgatgttgt aacgatcaat aatccagcgt
    14821 tcgccacgac tgccatagcc cgttacctga accacaaaac ggcgatgacg tcccgcctgc
    14881 acatccactg tcgccacaag gaaattaacg ccatccggca cactgcggga aggaactggc
    14941 tctgcccgct gctcaagcag ttcacttttt cgttgctcca tgctggcacg aggaagataa
    15001 ggcaatcccc agtcggtgtt gataaccgcc ctgagtgttt cttcgcttcc tgtcgcttca
    15061 tactcctgtt ctgcagtcag taatttgtaa accagttgcg cccaggtctg atacgcagca
    15121 gctggccctt ccatccagaa actggcgata cgggagcggc gcggttcacc ggaaacgttg
    15181 ccgttacgat caatgacctg accttcacgc aaccagactc ctgcactatt gagctcacgc
    15241 tttttctccg cagtgataat gccgctgcag tgcgggcaaa gtagatacgc cgcctcactg
    15301 gctttaaagg gatccggttc attacggtag ccggtcatgg catccatggc tggctgaaaa
    15361 tattcaccgc agtgcggaca tgaccagtac cagcggcggc ggtcaccacg attgtaaagg
    15421 gaaagaatac cagtcgtcgg tggcgcttca tgaggcgact tacgtcgcca tttgctgtcg
    15481 cagatgtcac gtcccggcga gctctccacc agagtcatcc cagcggacat aaatgtggtg
    15541 gtacgttttg aggccaggga gaaaccatca ccctcgctgt cgatattctc cggaaaacgg
    15601 tcgtaatcgg ttaaggcgac aaaccggtaa tccgacgacg acataatgtt gaccgagggc
    15661 caaccaattt taaggaacga gccatccctg aacgtcttat catggacatt attgtcgtta
    15721 cgacgtggac tcattctttt ctttaccgcc gcactgcttc tgaacgttct gtcgaggcgc
    15781 tttttagaat gctcgcgggc cttatcttcg gtcatctgca caacgagcat gtccgaagga
    15841 tcgcaaacga tggtatagac aatccatcca tcgatcagac caatggtctt ccctgttcgc
    15901 gcaggaccaa caaaaatcac cgcatcgtat tcacgcgatg ccaggcagtt catgggctca
    15961 atgatgtagg gtgtcagttc aggatcccat ggcaccgagt taccagcccc cttgggaaca
    16021 cgcatgaatt ttttaacagc ctccgaaatc ggcatgcgac gtggtgggga aaatcctgcc
    16081 gatatgtccc ttcccaaatt tcgggctgat gaaaaaccca ttattcctcc tagagactct
    16141 ctccttcctc atcaggaatt aattcagcag cacaagcctc gtaggatttt tcctgaagag
    16201 tgtatcgcag gtcatcaatg gcctgctgta caacgccgac ggcctgagga gtcagggcgc
    16261 aatcgcgttc aagaacatcc ggaattgtct ccagaacctg gacgacagcc tttctcatgg
    16321 acgaatagac gatgactact tcatcaacgg ggatgagttt tcgctgctcc ttttccagct
    16381 tgatcctttc attttcagat tggtaccagt cctttctctc tttcggctcc atacgggatg
    16441 gatcatgaac agagtctgct gcctcatgct tcacactaaa cagggctggc ccgacatgct
    16501 gcagggcgta aacggggttc cccctgacag tcgcagccac aggagtgtta gccgcgagga
    16561 gccgtttttt tactgtgtcc cggtgaagcc caaaggcctc agcgatttta aaaacactcc
    16621 agtaataagc atcaccgatc ccgctcacat ttgacataag caactccatc tggcaggtga
    16681 aaatcatgtt tatttatata tttcaattaa ttgcaaactg gtctaatgac agggagaaag
    16741 aaatattgta caggtgaaaa gagaaataac ttttaattat caataaatta ccaaacatgc
    16801 tgccgccgcc atggaaatgc aaaaactagc ctttttccgc gacgctcccg ccccgtggca
    16861 ggggccccca ccgggaggac ccgacagcct gacagcctga cagccgtgat gagcatctga
    16921 tacagcgctt tacataatgg cataggaata attcagaagg acatcacagc atgcccacac
    16981 aaattagtgt gagtgtcctg tttcttccta cgcacaggac tgacgagcat gaggggaaaa
    17041 tatgcgaacc ataaatgcct catcttcagc aatgcaaccg gcatccgaac aggacgatat
    17101 tgtaaattca cctaattacg aggacattgc agtagttgaa ttgcagctct gtattagcct
    17161 gacagtgaca gaatgcgata ttgactctgt cacaggtgaa atagtttgaa tgattagcag
    17221 ttatggtgct cagtcaacca ccagggaata atccttcgaa ttcttatcgt gcttcaccaa
    17281 cgctgcctca attgccctga atgcttccag agacacctga tgttctatac atgcaattac
    17341 aacatccggg taactcatag aaatggtgct attaagcata ttttttacac gaatcagatc
    17401 caacgagact tcatcagcag attgttcttt attcattttg tcgctccatg cgtttgctcg
    17461 tcatctagcg gttaaaatat tacttcaaat cttactgcat aaaagaggaa aagtatgaaa
    17521 gtttgagtac atcgacctta catacatctg tcggttgcat atccctcctg gatgccagca
    17581 aggctcaatt ttgttacgca atcaacacta ttcattaaaa acaggcttaa tatttgacat
    17641 aaatcatcaa caaaacacaa agaggtcaga ccagattgaa acaataaaca cgataatgct
    17701 aactacgcgc catcgtatca catggaaggt ttaccaatgg ctcaggctgc catttttaaa
    17761 gaaatattcg atcaagtgcg taaagattta aactgtgaat tattttattc tgagctaaaa
    17821 cgtcacaatg tctcacttta tatttactat ttagccacag ataatattca cattgtgtta
    17881 gaaaacgaca acatagtgtt agtaaaagga ctcaaaaagg ttgtaaatgt taaattctcc
    17941 agaaacaaac atcttataga gacctcctat aataagttga aatcaaaaga aatcacattt
    18001 caacaataca gggaaaatct tgctaaagca ggagttttcc gatgggttac aaatatccag
    18061 gaacaccaaa gatattacta tgcctttgat aactcattac tatttactga aagcatccag
    18121 aaaactacac agatcttacc acgctaaacc ataacgtccg gcttctctca ctcctgagcc
    18181 ggactgcatt ggtttaataa aaaccatcaa caattgtgat ttagatattc ggaaccattc
    18241 aaatataaca aaaccccgaa aaaacgaggt ttatggataa attttattat tgaatacatc
    18301 agattaaatt aatcttgaca tcatagcttt caagacccgt cattttttcc cgtgcggtaa
    18361 actgaatact ggtaacttct ttcccggtct ttttcttaag ttcaataatt ttttttgtta
    18421 tatattcaga aatatctgct tctgcttttg tttttaagtt ttcaatattc atcatttcct
    18481 cttttagtct gttatgactt tccagttaca cagtaagtcg attatatggt gcaaacgtgt
    18541 aaaagataag atgaaacatc gcaataatca acatacgata gtctaaattt tacacaaaca
    18601 gacaaagaga attttcctga attatcaata caatagcatc aaatcaactc aagagcctta
    18661 ttgctgcttc cagaatttct tctgaagtaa catgtcgatc cgcggctaca taaatgactt
    18721 tatgatctcc ggtcagagat ggaaaccctg cggccattac agtaaggtgt gttttttcgc
    18781 catttggata ttcacgcatg atggtgttaa ctccagtcat cgctggcact accactgctg
    18841 gttcagagtt aaaaaaaact atgatttttt tcatgatgtt accgtagtat gtgagtatcc
    18901 atcgaataga taccaagcaa aaaagctccc gaaggagcct tcattttcac ttttttaaat
    18961 ccaacgacag acggctggca tttaagtatt gtgaaatatt atcaaatgta atcatcattg
    19021 atttacaaaa gatacatttt gccccgaaag gattcatgtc agaaacatca aaagatgatg
    19081 ttctatactg ggaaccatga caacacgggc atctaaagtg aatatggttt gtaatattgt
    19141 ctacctcaaa gcgccactac atgaacagcg gcaggacctt taggtccgtt ctcaatacca
    19201 aattcaactt cctgattctc agttaatgtt ttgaaatcgt tgctctgaat tgctgagaaa
    19261 tggacaaaca catctttgct gccatctttc ggcgtgatga aaccaaaacc tttttcaggg
    19321 ttaaaccatt tcactaaacc agtcattttg ttagacataa ttattacctt ttgaagaaat
    19381 tagcccttgg gcagaatggt ccgaaaaaaa atatcagaga gaaaaaccaa caaggaaatc
    19441 tcaagaggta caaataataa aattataaca atgactgctt cagataaatt tgtaacaaac
    19501 cagaacacca ttaacgcatg attaaccacc catagcaagg attacttttg taaagaaaaa
    19561 cacagcaatg aaagaatagc tttatttatt aataaaacgt gtcattctga ttaagacctt
    19621 ttatcttacc cttaagattt caggaatttt ggctcatgga agagtccttt ttatttaaat
    19681 tttacattcc gcgatgtaaa tgttccgatt taatattacc ctacatttga tgctttttat
    19741 ctcttaaaga ttcatagatc tgttgacaag tcactcctgc gatgtagcgt tcgtcagcaa
    19801 tttcagcata aagctgagct tctgctgcaa tatctccgag catgttggtg agcattcctt
    19861 cggcggtttt ggttgttttg cctctgacgg cagcggcaag atctgcggta tgcttcgctg
    19921 cgtcaaggcg tatggcatat ttttttgctt cggcacgcaa ctggttaaca ctatcagaca
    19981 gataagcagc cctggcagaa atttcagcag atttctgttg tgcatcttta acagcctcat
    20041 cacgggctat agttcgcccc tgttcaatta ttcgagcagc aaattgagca tttacctctt
    20101 gtgataatgc ggcagcatca cgttccgccc attttttttg ccatcctctg tcgctccaga
    20161 catttccgac gataaatcct gacaacacga gaaaaatcac catgaatatc tgattcactg
    20221 ttctatcccc cagcaggtta atgcgctctc ctggtcacga cgaataacct gaccgtaaca
    20281 gttatttgaa cgaatgcggc aatcgcgtcc gccatcctta atccaccagc gaatcgcttc
    20341 gcatgcacct ttacgatcac cagcattcag ccgcttataa aacgtcgacg ggaaacactt
    20401 accggggcca atgttatagg gacaaaatga cgcgataccc gctttttgtg gttcggtcag
    20461 tggtacttta atattgctct ccacccatgc cagcgcctta tcacgctcaa tggcgttgac
    20521 ctggtcgcat ttttccttcg acagtttcat attgggaaaa acggtttttc catccaccac
    20581 tgtggcaccc cgacagatgg tccatatgcc agaaccatcg cggtatgcca ttgtgtggtt
    20641 accttctttt tcgtccagaa actggtcaag tatctgagga gcagatgcgc cagcaccaat
    20701 cagcgccaga acggcagccg acaggccgta tctgattttt gtgttcatat atatttatga
    20761 tgaggacgct cgtgcttatt ggcaggattt tcaatcttaa aggagtactg atgctgcaga
    20821 taagactcaa ctttttctga caatttttct gctacttcca ggaagacttg ccggacgctc
    20881 cttctggctg ctgcctcata aaactccagc gcagctcctt caacacggtc catggcgaca
    20941 tccaggtcaa aaatttcacc gtcaaagcgt tccttgtcct gtaaggctac agttaccgta
    21001 actttattct caaaattacg gactcctttc acaaccagtt catagtcttg agtcattgga
    21061 ttactctcct ctcgcagcct tacgcctgtc ttctttaatc ttgaaataaa gatttgtcag
    21121 atacgtcagc aggccaaaaa ccaggctacc cagcacaccg attgcagccc actgtgacgg
    21181 agttacttta tcgagtaact gtaatgccca gaaaccagca ttacccgccg atgtgccata
    21241 ggcaacacct gttgttaact tatccattga tttcatatcc tcaccccgat gtacacggat
    21301 ggtgcaatat gtttgaaaaa gatcggagtc tacggggtag ttttgacagc acacgttgtt
    21361 ctcaacggcg ctaaagaaac atacacatta aaaatgtgag taattatttt gaaagaaagt
    21421 catatataaa ataataatac gagaaatgtt ttcatattta gtgtactgta tacggccatt
    21481 atacaggaaa agcctatgtc agaacgtaaa gactcaaaat cacgccgtaa ttatctcgtt
    21541 aaatgttcct gcccaaactg cacccaagag tcagaacaca gtttttcaag agtacaaaaa
    21601 ggtgcccttt tgatctgccc tcattgcaac aaagtattcc agacaaatct taaagctgta
    21661 gcctgattga ttttattagt aacaagtatt ttttatattt taataatata tttaaagcag
    21721 ataataaaaa acccgcctga gcgggtttga gattgtggtg ctttttgtgg gagtcatcca
    21781 cttacgcact ttgttttgcg atgccagcag ttagcttctg ctgtgaaact attcatgcag
    21841 caaacctgca cttcaccaca atggttagca tacttttcct gattaagatt ttgccaaata
    21901 tgctagccat tgtttcatgt attggacctc cttacttttt attaaagaga tccaatattc
    21961 actactctgt ccgtatctct actcaggcat cagccttctt cgttatcgta tacagacgag
    22021 cgatgaattt taatcagtaa tgatgacatt tgctgctgca ggacctttag cacctctctc
    22081 tatagagaag gtaacctttt gaccttcaaa taaggttcga taattatcat tctgaatcgc
    22141 agaaaaatgc acaaacacat ctttactacc atcaacagga gaaataaagc cgaaaccttt
    22201 atcagcgtta aaccatttta ctaaaccagt cattttattt gacattctac attccttaac
    22261 ttgagccttt cggcataaat ggtttgcata acagaaacga cttcgtactt aattggagag
    22321 actcaaagaa ggaataagtg aataacacct gaaatgagaa ctgctttagt aaactacttc
    22381 gtatatcgtc tgttcttcaa accgacgcaa tcattaacgc atagttgaac atatgaagca
    22441 atgtttattt tagacatcca gccatcttca accccatcaa aaaactatag ctttcttcag
    22501 gaacgtgtgt atagtgcgcc aagttatcag tattaaggaa tttttttgtc ccgtaaaatg
    22561 acaggaattg tcaaaacctt tgacggcaaa agcggcaagg gtcttatcac cccatccgat
    22621 ggtcgtatcg atgtccagct tcatgtttca gcgctcaatc tccgcgatgc agaagaaatt
    22681 accaccggat tacgcgtgga attttgccgg ataaatggtc tgcgtggccc ttcagctgcc
    22741 aatgtttacc tttcatgagc tatattaaag ctttaatttc aggccccatc ggatcacaca
    22801 tggagagttt ttatgaataa ccccgtctgt cttgatgact ggttgattgg ctttaaaagc
    22861 ttatgctgta ctttggccgt aatagctctg ctaataatat aataagcaga ctcattgtgt
    22921 ttagggacat tgtactggaa gaaaacattt taaacatcag gcaaataacc aagtcaccag
    22981 ctaaataata agttaacaga catgagtccc gggatgagat tcaacattac cattgcccca
    23041 tttaaagcac aaaacccgct catcagcggg ttttctactt tttcttaacg tcgggtatac
    23101 aaagcccatc gttgaaaaaa ttttatccat attttttgaa aaatgcaaac atcatgtcgc
    23161 catcttcagc aaaaatcatt tatctcgtca ccttcctcaa ttgcgcttcc gcgtatgctt
    23221 cttcctgcca gcactttgtt accagtttac caatgacgtc cgcatacccc ttataccact
    23281 gataatcggt caggtctggt accagcttct ggacatgacg tcgtgccagc gtggtcggta
    23341 aacgactaaa ccggtttcca ttacaacgcc cacaaatctt atataccggt acgccatgaa
    23401 accgggttct tttttcatcc agaacaatcc ctttaccctt acaccctctg cacgctgtgc
    23461 tgacttcacc cttaccatgg caatgctgac atagttcctt cacccattct tccttgatta
    23521 cagattcccc gcgtcggtag tgtttcacca cttcgcgcaa tacattataa aatcccgtac
    23581 ctgaacaatg ctcacagcga gccttacttg ccgcagacct ggagtaatca gcaaaggcaa
    23641 aattcacgag gtaaggaata atctgtaacc ggatttcttc actcaatttg ttcaatgtcg
    23701 ggttatccag tgccatcgcg taatttagca ggccttcaat cgcaaactga gggtcctgaa
    23761 caccaacttt tgccagaaat aaggccaacc caagtggtgc tttcgactgc accatcccct
    23821 gcgctgccat tacatccgta attgttaaac aaccggtgcc tgtcgctgga gcgtcatcgc
    23881 tcaattttgg agattttggg gagtaatatt ttggtaaggc ttcaaggttc atgctcgttc
    23941 tccacttacg ccagtacgcc aattgccagc gcgcgatcga taaaacgaaa tatcagctcc
    24001 agttgggagc catacttatc ttcaaatgcc actgtatccg tatgcagctc gttgtgatgc
    24061 tttctgcaca aaggcaacac aaagagatca tgtgcttttg ttcccattcc gccctgcccg
    24121 tgaccaatca gatgatgcgg atcgtcggct ggcataccgc agcaagcaca cggctgtgtc
    24181 ttaacccaac gtgtgtattt ctccttaacc cagcggcgac gtttaggcag cttcatgaaa
    24241 gattccggag actctggatc aacggtgatg cttaccaccg tcttttcctg tggtgatttt
    24301 tgttgctggt gggcgtaagg caacggtgca agattttttg tgcgttgttt caatatgctg
    24361 gtggcggtct gctctcccgg tacgatgtcg ctttcgcggt acaccgagca gattttttcc
    24421 gctggtaatc ccagcgaacg acgcgataca gcctcaggta gtgcatccac cacctgattg
    24481 cagaccgccc accaggataa ttcagccaaa gataattccc gctcctgcgt accgcttatt
    24541 gcgtgacgga tgacgtcaat cacccatgct gtcagatttt gttgagcaag cagctccagt
    24601 gattccgatg tctggtcacg cagttggttg tcgcagtgcc agcacaacac cattgcgccg
    24661 gtaccataac ggtgaatgac tgtttcagtg tgatggtaat caccattagg ccactggcag
    24721 gatgtaacat gacgtaatag ccagtcggac aatgcgccaa cgccgccagc agcacgaatc
    24781 acccgttcgt tactaaaaaa cggcagcaat gttttgtctt ccgccagcgg ctggcgaacg
    24841 gcaggaacga ctccggatgg cagattacgc atgctttttg gttccggttc caccagcact
    24901 cgaggattat gaaatatctg tatggattca cggcccggct taaggaccac cagcccaagc
    24961 tcaggcacca gaacaggtct aagtaatacc cgcacgttac ctccagatcc gttgctggaa
    25021 agtgcgggac gcacgtggtg ggcgttcgga ataaggcagc ctgacagaga ttatccagtg
    25081 ccgatagtcg agactgagag ctttcttaac ctcgaacccg cgcctgcggt aagaatgaat
    25141 cagccattcg gcctgttctg cagtgcatgg agggtgctgg aaccattcag acttgaatgc
    25201 gtgagaatac cgcccgtgcg tgcaggcaag aacgggcgaa ttatcagaat tgtaatattt
    25261 tgcgttgcgt gccatcggtt ttctccggtg gcacggtgtt actcagcggg agttcagccc
    25321 cgcgcaagat tgtagatgag tttattctcc tgaaaaagca gaaaagccag cttttattcc
    25381 gatctctttc aatgcctgta atgaagtgac aaactcacct tcgcgcaaga taaatccgtc
    25441 cgtgacccga gcatccacaa aattaattaa cgcagcccca ttctttcgca aacacataat
    25501 gcggtaatga ctaacaagat ttccattttc aacgcacaca gcatagaggc catcttcaca
    25561 aaaaatttta cgcagttctt cgatgttcat catcagaatc cttccggata attagctctc
    25621 ccctttaagg gaccatccct cttatccctg cgcgctactt aagtattttt gattctattc
    25681 cggcaccgtc cagaacttca aacgcgttga aaataaaaac aaaaacccgc cgaagcgggt
    25741 taagtgcggg tgcgttgagg atgcctgcca catcagaggt ggcgagggat ttctcccccg
    25801 ccgggtctct tactcctcag gttcgtaagc tgtgaagaca gcgacctccg tctggccggt
    25861 tcggattcgt acctcgcaga ggtctttcct cgttaccagt gccgtcacta tgacggttaa
    25921 acagatgacg atcagggcga ttaacatcgc cttttgctgc ttcatagcct gcttctcctg
    25981 tcaacgcaaa gcagaagtgt caccttcggt gcgaaacaga gatgtcatgc tttggttcag
    26041 agaatgcgtt tgaccgcctc gctatatact tccgagcgtt ctcttttccc aacagaaatc
    26101 acgaaaacga caactttctc gtctataacc tggtatacaa ggcgatagcc tgaagaccgg
    26161 agcttaatct tgtaacaatc aggcatacca cggagcttgt ttgcttcaat ccggggtgac
    26221 tcaagtactt caaccagctt ctttttcaac tgttcacgta ccgtcgagcc cagctttcgc
    26281 cattccttta gtgcccgctc gtcaaaatcc agaaaatacg ccatcagagt tcatccagcg
    26341 tcacacgtac tggcttagga ttacgaagcc gttctttcac tatctccaca agttcagcat
    26401 cttcatcact caggagtgtc tgtttgaacg gcaagcgttc attgtcagcg atatactcga
    26461 gcatgagacg aagcgcttca gaaggagtta cacccatttt ttcaagcgcg gcgtaagaac
    26521 gcgctttaag ttcatcgtca atacgcaggt taatgctacc catgtcttac acctcttgta
    26581 attacaaatg tcattacaag tatcgcacta caacatgctt agggcaagtc acgaaggaag
    26641 tcagaaagta gtcgtaagaa cggtgatcac tgtccgcttt gtgccaggag cagccattgc
    26701 taagtccatc ctgtattgtg caggtcagct cgtttttaaa gagtccggcc atcatcttac
    26761 tggtacagac accatatact ttgtgacggt caggctacat atgcacaact caacttattc
    26821 atctattttt tgctttagca tgtcagtgtt gctttctcgt cggcgggtga gcggtgacct
    26881 gacctgtcga taaaggaacg tagcacgttt tatgcaacac ccgcatgcgg cagaaaatta
    26941 ttgccgaacg tttacccctg tcaacaagct ttactttctg aggcgcgcca gcccgcgagg
    27001 aaaacaatct gaacatcaaa caattaatga cacaagaaat acgattaaag atttttttgt
    27061 gcatgccgat agtgcttttt taaaaggaga aatctatgtc tgtcacaatt cagggaaata
    27121 cctcaaccgt tatttcaaac aactccgccc cggaaggaac atcagaaata gccaaaatca
    27181 caagacaaat tcaggtgctg actgaaaagc ttgggaaaat ctcatcggaa gaggggatga
    27241 cgacacagca gaaaaaagaa atggctgcat tggtacagaa gcaaattgaa agcctcaggg
    27301 ctcaactgga gcagttgtta aggcagcagg cagagaaaaa gaataaagac gcgacagttc
    27361 agcctgataa aaaagaagag aaaaaagacg atacaaatac cgctggcacc attgatattt
    27421 acgtctaagt gacagccgta ttgtggccct catcgggcca cttttcgcca tcagcctttt
    27481 ctttaaagac atattatctt tgtatcattt ctgatagtta acattacaag atataagtaa
    27541 tggacgcact cccaattagt ctatttaaat cgccacgagt ttaactgaca acccatgatc
    27601 aattatgaat tgcaactatt tctgtagtca cttttgtggg gacagtccac aaaactgcca
    27661 acttccgctt cttgctctta gcggacatta gcataggcta tttaccataa cgcctcatta
    27721 cgcgcaccgc ccagactgac tcagcgcgtt tctggcatat ccccggtaaa acaagtaaca
    27781 aaccacccga aaatgaacat cagaaacgcg acttaagaat ctaccctatg aatggatatg
    27841 cactcaaccg aatcgatctt ggtttcaatc tattttatcg ggatcaggct tctttttagg
    27901 taacttcggg ggcttaactt gctgatgact ttgcgttcgg cgcgtaagcc agggatggtc
    27961 agctttagat ttaacatagt attttgagcg taaatcaata cgggcattat ccactcgttc
    28021 atggacactc ttttcatcat ccagtggtat aggctccggg ccatcaacat acttttccca
    28081 gcccagcgct ttcccgtcat acagaacgtt aatttcaccg tcaaagttct cacatacagt
    28141 aacaaccgaa tgcctaagtc gatatccccg gccctcactg cgtacctgaa acgcactgct
    28201 tttgtactgg aaagtgagat ttttagacag aacgcgcttc gcctgtaggc tgaagatata
    28261 acccagttcc tcttcagaat ggtgcacatc aagatgagca ttatcagtag tacgaggcga
    28321 cgtagcgaac cggttgttat aggcttcaat aaaggtcggc aaccatgcat ttgctgtttc
    28381 aatatcactg atattctgaa gccgcatttc tttgaccagc ctgtcctgta gtgtctgatt
    28441 ggcgcgctct acccgccctt ttgcctgcgg gctgttggca tggattggct cgatgcccag
    28501 tgtctttatc gcacgagtga actgggtcag ctcaccttcc cgctctgggt tatttaccct
    28561 gaatatactg tgtctatcag agtagagagc gagcggtacg ccatgatcat taaggtaacc
    28621 ccggagggtt tccatgtaag cccgggttgt ttcagcaggc acaaaacgca acgccatcaa
    28681 cgcactggtg gcatcatcaa tgaaaacgat cagtgtacat ctggggcctc gattttcaaa
    28741 ccagtcatga ggtgagccat caatctggat cagttcaccg taagatggtc gtcgcatgcg
    28801 gcgttgatat atacgggcaa ttttacgacg gcgttcacgc cataacccct cttctatcat
    28861 ccactttctg agagtttcaa cggataaaga taaaccgtgt atctcgcgca atttttcgca
    28921 cgcaaacgta ggtccaaaat cagcgtaacg gcctttgagg agtgaaatta ctgttgctct
    28981 gaattcagta gaaaaggaat tattaggacg ctttccacgt cggtgggaaa ccagaccaga
    29041 aggcccttca tttctatacc gttgcaccag acgtttaacc tgccgaatag aaatgccaat
    29101 gcgtgccgca gcttgttcct gagtaatatg tcgattaatt gactcctgaa taatctgaag
    29161 tcggtggagt tccttatgac tcatcgtaac agtctctttg atcatgaaaa atcccccaga
    29221 gaattgtctg gggacatttt agaatggttc aaaggggaca ttacagcttg gtgttaacaa
    29281 tagcctgctt ctccttgcct tccggcactc gcgacagatt atgtttagac gactgtcgta
    29341 gcgacgtatt tctccgtcag gtaatgacca gataaggtcc ggatcaacca cagcaggttt
    29401 cttcaccttt gccctcgaga gttttttgcg ggcgttttgc cagtccttac gcgcctgttc
    29461 agacgggaat aacccgtaac cagagctgta tacatcgcca ctggcaacca gctctctggc
    29521 cagaacgctc atcagatatc ttgttgcccc agttttagct tccagttgtc gtaacgtctc
    29581 gcgcccactc tggcgtacga gttccaccac ctgcccttta attttttccc gctcttcttg
    29641 tgtaaaaact tttgccacaa gtcctcctga acattacttt ataccctaaa atcaacactt
    29701 atcccctgaa accaggcgga atttctgtat ccggttcaga aatatgattc acacaacgct
    29761 gtacaggtga acgtcccagg cggatgacca gttcgtccca tttttcgcgg agttttgacg
    29821 ggctcatgat gttttttacc cagaattgat ctcgctgtac ccgaccaaac atttcgcaaa
    29881 tttgtctgtg gcttctgcca tccagcatcc gcattgtgcg cacgtcattg gcccaggctg
    29941 tccagttagg ctcttttggt cgcatgatct ctccatcgtc gctggcagcc tgttcgtaaa
    30001 ggttcacgat ccgcccccaa atccactgcg cacacgccaa atcctccctg ctaccccact
    30061 ggcgtttttt cgcactaaac acaaccgcgt cggggttccg ggttaaaaaa tcctgttcag
    30121 cggtctgctg gtccggttgc gaagcttccg gacgaaaagt gtttttattc tctgtagtaa
    30181 tctctgttgt attctctgta agatcatcag gccattttga cccgatgaca ttgggtcgtt
    30241 ttgaaccaat ggagcgtttc attttgacct cttccatcgt gtcattttga cctgatggag
    30301 cggcgcattt taaaccgatg gattcgctca atttgccacc atctaaaagc tcgctcccgt
    30361 agttgatcgt gtagaaattg gtcatatcgc gctttgattt attgagcttt tcacaacgca
    30421 aaagccccag cgttttcaga cttgcaaacg cgcgctttaa cgttgactct gaccagaatg
    30481 ggaactgttc cagccattgt tccgttgtgt tgtaaatcca gcgaacacca tcacattcca
    30541 tgccggaatt ggtatctctc aaccagtaat gcaactgctg caacacaatg gcttcattta
    30601 agccaatctt catcgcaagc tgtgtgttta taaccagtgg gcgttcagca aaaagaaggc
    30661 tcataattcc atccagcttt ttgttggtat tgctgtcgat acgcaagttt gaaagcaatt
    30721 gctttttcta taagttcgtc agtttcacga tccactacgg caggatcagc aaaaagcagt
    30781 ccggactcca ccacatcgcc atattctttg tttaacccgg cgatcatgta cgtgatgctt
    30841 tttccgtcac taatttcacg atacaacctg aaatcattaa tccgaatagc ctccataatt
    30901 gcaggcacta gcgccgtgaa cttttcacgc ttatccctgg tgtcgatagc cttccagcgt
    30961 tcgaatatct tcactcgatt aacgccaagt gctcgctgat caaccgcgcc accttcatct
    31021 gtgacacgct gaacatcgat gttcgggcgc tctttcaaag cccagaatgc ttcagtgatt
    31081 aatatcgtcg cctgctcctg tgtcattcct ggtcgacata tccaggcatc cagagcctca
    31141 cgagcctgtt caggagtgat tttcattgtt caaccgcccc gcccgcttcg tcttacgata
    31201 ttcgtcataa accttgggat catacagaag ctcgccgcca gatgcctcct gtagacgcat
    31261 cgcgcgacct tcaggaacca gctcttgcca ttgagaaaca gcagatgggt caacgccagc
    31321 agctttcgct actttggctt tcgtaccgta aaaattaatt acgtctgatt taaacatcgc
    31381 acctccaata ttgagttttc tcaatgctaa tcactcaagg aatctcaagt caagggttat
    31441 taagatatct aaatatgaac gagaaaactt taggtcaacg cattagagaa agacgtaagc
    31501 aggtaggctt aagtcaaaac ggtttaagca aagctgctgg cgtatctggc tcatcaattt
    31561 cattatggga aagtgaccat acagccccgc gcgggcaaaa tttgcatcgc ctcgccgagg
    31621 tattgcaatg ttcaccaact tggatactgt ttggcgacga ggacaaaaca ccagctcccc
    31681 ctgtttcact cgatagtgcc ttagacttat cggaagatga gttagagatg ctgcgtttgt
    31741 atcgcgcact tcctaaatca gagcaacaag cacaactcag cgaactccgc gcccgcgttg
    31801 agaattttaa tcgcctgttc accgagctat tagaggctcg caaacgcaac aagcatcaat
    31861 aatcatccct tcacaaaatt ttaaagcctt acatttcaat gtattggctt tatcttgcgc
    31921 caacacttga gttttctcat caaaaaacct tgacgaaaaa taatgagaaa actaaattac
    31981 ctccatcaag acaccgcacg gtgttctcag caaacagttc cgctaccccg gcgttaaggg
    32041 gaaatgaggt cagcatggat actatcgatc ttggcaacag cgaatctctg gtatgtggcg
    32101 tgttccccaa ccaggacggc acgttcaccg cgatgacgta taccaaaagc aaaacgttta
    32161 aaaccgaaaa tggtgcccgt cgctggctgg aaagaaactc aggtgagtga tatggatttc
    32221 aacacaatca tggaaaaggc ttacgaagaa tacttcgaag gccttgccga aggcgaagaa
    32281 gctctcagct tcagtgagtt taaacaggca ctcagaataa gaatgtgctc tcacaatgac
    32341 gcggagcaca aatatgagaa gcaaaatcag accgcagaaa attttgttct ggaacccgga
    32401 gaaacgcttt tcaaaattcc cgttacgtgc cccatttgcg gttttacatc agaagaactt
    32461 gacgactcct gtaacaatca ggaaacaacc aagtatgtcg aagatgatac cgagtgcgca
    32521 cgaagaacga ttatatccac gagtccaaac tccaggacca ataaatctca ctatgagagg
    32581 gtgattaatc cactccccca aaccaataaa aaagatgccg gaggacaaaa aacccaatgg
    32641 aacaacggga tatctgtcaa aaaaagacgt tccattaaag acaaacaacg cagcgccaac
    32701 aactgtaagc gctttatacc agtaatcaat tttcatgttc ttaagcggat ttattggtgg
    32761 ttgcgacatt gcttaatgaa tccttaaaac tgtggtgatt ttaaggatac cacctcgcct
    32821 gacgtggtta aaagcaggca cacaacacga aagcgcacgg cgaagttcgt ctcactgtac
    32881 ggtgtcgtta aatttaattc gaccgtgcgc ttccggttgt ggcaacccgc aaaatggcgc
    32941 ggcggtaagt atggcggggt tattccttcc ccgttgagga caccgggttg tcaggttgac
    33001 catacgctta agtgacaacc ccgctgcaac gccctctgtt atcaattttc tggtgacgtt
    33061 tggcggtatc agttttactc cgtgactgct ctgccgccct ttttaaagtg aattttgtga
    33121 tgcggtgaat acggctaagc gcacgcggaa cagttaaaac caaaaacagt gttatgggtg
    33181 gattctctgt atccgacgtt aattgttaac tggttaacgt cacctggagg caccaggcac
    33241 tgcatcacaa aattcattgt tgaggacgcg ataatgaaaa cgttattacc aaacgttaat
    33301 acgtctgaag gttgttttga aattggtgtc actatcagta accctgtatt tactgaagat
    33361 gccattaaca agagaaaaca agaacgggag ctattaaata aaatatgcat tgtttcaatg
    33421 ctggctcgtt tacgtctgat gccaaaagga tgtgcacaat gaattcagca tttgcgcttg
    33481 ttctgacagt ttttcttgtt tccggagtgc cagttgatat tgcagtcagt gttcacagga
    33541 caatgcagga gtgtatgact gcagcaaccg aacagaaaat tcccggtaac tgttacccgg
    33601 tcgataaagt tattcaccag gataatatcg aaatcccggc aggtctttaa aacagttccg
    33661 taataaacat ccgatttcat tcttatatgc cagcaatggc agggatttgt tcacccttaa
    33721 atctgtaatg aggtaaaaca aaatgagtaa agtctttatt tgcgccgcca ttccggacga
    33781 acaggcaata aaggaagaag gtgcagtcgc tgtagccac
//
LOCUS       DAZGOB010000044        32654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGOB010000044 DAZGOB010000000
VERSION     DAZGOB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.044"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(38..199)
                     /gene="rsmS"
                     /locus_tag="ACHLIF_002231"
     CDS             complement(38..199)
                     /gene="rsmS"
                     /locus_tag="ACHLIF_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO9606241.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(213..740)
                     /gene="priC"
                     /locus_tag="ACHLIF_002232"
     CDS             complement(213..740)
                     /gene="priC"
                     /locus_tag="ACHLIF_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO9606242.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            810..1187
                     /gene="ybaN"
                     /locus_tag="ACHLIF_002233"
     CDS             810..1187
                     /gene="ybaN"
                     /locus_tag="ACHLIF_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO9606243.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            1340..1891
                     /gene="apt"
                     /locus_tag="ACHLIF_002234"
     CDS             1340..1891
                     /gene="apt"
                     /locus_tag="ACHLIF_002234"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO9606244.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            2020..3951
                     /gene="dnaX"
                     /locus_tag="ACHLIF_002235"
     CDS             2020..3951
                     /gene="dnaX"
                     /locus_tag="ACHLIF_002235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO9606245.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    3284..3348
                     /gene="dnaX"
                     /locus_tag="ACHLIF_002235"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            4004..4333
                     /gene="ybaB"
                     /locus_tag="ACHLIF_002236"
     CDS             4004..4333
                     /gene="ybaB"
                     /locus_tag="ACHLIF_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO9606246.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            4333..4938
                     /gene="recR"
                     /locus_tag="ACHLIF_002237"
     CDS             4333..4938
                     /gene="recR"
                     /locus_tag="ACHLIF_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO9606247.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            5048..6922
                     /gene="htpG"
                     /locus_tag="ACHLIF_002238"
     CDS             5048..6922
                     /gene="htpG"
                     /locus_tag="ACHLIF_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO9606248.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVELEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            7103..7747
                     /gene="adk"
                     /locus_tag="ACHLIF_002239"
     CDS             7103..7747
                     /gene="adk"
                     /locus_tag="ACHLIF_002239"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO9606249.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            7983..8945
                     /gene="hemH"
                     /locus_tag="ACHLIF_002240"
     CDS             7983..8945
                     /gene="hemH"
                     /locus_tag="ACHLIF_002240"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO9606250.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKMYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(8942..9901)
                     /gene="aes"
                     /locus_tag="ACHLIF_002241"
     CDS             complement(8942..9901)
                     /gene="aes"
                     /locus_tag="ACHLIF_002241"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO9606251.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            10053..11357
                     /gene="gsk"
                     /locus_tag="ACHLIF_002242"
     CDS             10053..11357
                     /gene="gsk"
                     /locus_tag="ACHLIF_002242"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO9606252.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(11490..13166)
                     /gene="ybaL"
                     /locus_tag="ACHLIF_002243"
     CDS             complement(11490..13166)
                     /gene="ybaL"
                     /locus_tag="ACHLIF_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO9606253.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(13404..14624)
                     /gene="fsr"
                     /locus_tag="ACHLIF_002244"
     CDS             complement(13404..14624)
                     /gene="fsr"
                     /locus_tag="ACHLIF_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO9606254.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            14842..16494
                     /gene="ushA"
                     /locus_tag="ACHLIF_002245"
     CDS             14842..16494
                     /gene="ushA"
                     /locus_tag="ACHLIF_002245"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO9606255.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGKMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGRLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(16531..17010)
                     /gene="ybaK"
                     /locus_tag="ACHLIF_002246"
     CDS             complement(16531..17010)
                     /gene="ybaK"
                     /locus_tag="ACHLIF_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO9606256.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17214..18008)
                     /gene="ybaP"
                     /locus_tag="ACHLIF_002247"
     CDS             complement(17214..18008)
                     /gene="ybaP"
                     /locus_tag="ACHLIF_002247"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO9606257.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            18146..18487
                     /gene="ybaQ"
                     /locus_tag="ACHLIF_002248"
     CDS             18146..18487
                     /gene="ybaQ"
                     /locus_tag="ACHLIF_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9606258.1"
                     /translation="MKQATRKPTTPGDILLYEYLVPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(18601..21105)
                     /gene="copA"
                     /locus_tag="ACHLIF_002249"
     CDS             complement(18601..21105)
                     /gene="copA"
                     /locus_tag="ACHLIF_002249"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO9606259.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            21367..22299
                     /gene="glsA"
                     /locus_tag="ACHLIF_002250"
     CDS             21367..22299
                     /gene="glsA"
                     /locus_tag="ACHLIF_002250"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO9606260.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            22302..23594
                     /gene="ybaT"
                     /locus_tag="ACHLIF_002251"
     CDS             22302..23594
                     /gene="ybaT"
                     /locus_tag="ACHLIF_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO9606261.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            23625..23722
                     /locus_tag="ACHLIF_002252"
                     /pseudo
     CDS             23625..23722
                     /locus_tag="ACHLIF_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23719..24126
                     /gene="cueR"
                     /locus_tag="ACHLIF_002253"
     CDS             23719..24126
                     /gene="cueR"
                     /locus_tag="ACHLIF_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO9606262.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(24127..24585)
                     /gene="ybbJ"
                     /locus_tag="ACHLIF_002254"
     CDS             complement(24127..24585)
                     /gene="ybbJ"
                     /locus_tag="ACHLIF_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO9606263.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(24582..25499)
                     /gene="qmcA"
                     /locus_tag="ACHLIF_002255"
     CDS             complement(24582..25499)
                     /gene="qmcA"
                     /locus_tag="ACHLIF_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO9606264.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            25645..26322
                     /gene="fetA"
                     /locus_tag="ACHLIF_002256"
     CDS             25645..26322
                     /gene="fetA"
                     /locus_tag="ACHLIF_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO9606265.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTNGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            26309..27088
                     /gene="fetB"
                     /locus_tag="ACHLIF_002257"
     CDS             26309..27088
                     /gene="fetB"
                     /locus_tag="ACHLIF_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO9606266.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(27151..28005)
                     /gene="cnoX"
                     /locus_tag="ACHLIF_002258"
     CDS             complement(27151..28005)
                     /gene="cnoX"
                     /locus_tag="ACHLIF_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO9606267.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAETVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(28066..28875)
                     /gene="ybbO"
                     /locus_tag="ACHLIF_002259"
     CDS             complement(28066..28875)
                     /gene="ybbO"
                     /locus_tag="ACHLIF_002259"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO9606268.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(28865..29488)
                     /gene="tesA"
                     /locus_tag="ACHLIF_002260"
     CDS             complement(28865..29488)
                     /gene="tesA"
                     /locus_tag="ACHLIF_002260"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO9606269.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            29459..30145
                     /gene="ybbA"
                     /locus_tag="ACHLIF_002261"
     CDS             29459..30145
                     /gene="ybbA"
                     /locus_tag="ACHLIF_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO9606270.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            30142..32556
                     /gene="ybbP"
                     /locus_tag="ACHLIF_002262"
     CDS             30142..32556
                     /gene="ybbP"
                     /locus_tag="ACHLIF_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO9606271.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         7748 a         8620 c         8791 g         7495 t
ORIGIN      
        1 acaatcttag gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgc
       61 gcgttaattt cttttcataa tcgcgtttta caatatccag cgcgcgcagc acggtgcggg
      121 ctgggatctg attttcttcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag
      181 gggcattttc cagtgacata ctctctccat tgctagcggg ttaagcgcgc tagcctgttt
      241 tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca
      301 cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg
      361 gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag
      421 cgggcaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc
      481 gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt
      541 tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcatc gagacaagcc
      601 tgcagtgttg tcgcacgggt ttgaaaaagg tgtctgtcaa aacgggcact tagcgtggca
      661 aattgcgcta ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc
      721 agcagcaggg cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt
      781 agccactctg taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct
      841 ggctggcggt agtgctgggt acgctgggcg tggtattacc ggtattaccg acgacgccgt
      901 ttatcctgct ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc
      961 tgtaccgctc atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc
     1021 gcggcgtcaa accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt
     1081 tcgtccagat gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct
     1141 atatgtggcg aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg
     1201 cagttgcaat tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg
     1261 gtcaaaggtt aaacaactgt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt
     1321 aacaagcagg catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag
     1381 catcaaaagc attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt
     1441 actggaagac ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa
     1501 tgcgggcatt accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt
     1561 agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga
     1621 aaccatcagt gaaacttacg acctggaata cggcaccgat cagctggaga tccacgttga
     1681 tgccatcaaa ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac
     1741 tatcgaagcg accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt
     1801 cattatcaac ctgttcgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag
     1861 ctacagcctt gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta
     1921 cggacagcac aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc
     1981 ccttcgtgaa tccaccttcc agcgtttcag agcctgccaa tgagttatca ggtcttagcc
     2041 cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca
     2101 ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggtacccgt
     2161 ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc
     2221 attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt
     2281 gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg
     2341 ctggataacg tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa
     2401 gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg
     2461 gagcacgtta agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg
     2521 tcgcgctgtt tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcaactt
     2581 gagcacatcc tcaacgaaga acatatcgct cacgagccgc gggcgttgca attgctggcg
     2641 cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc
     2701 ggtgacggcc aggtttcaac ccaggcagtc agtgcgatgc tgggtacgct tgacgacgat
     2761 caggcgctgt cgctggttga agcgatggtc gaggccaacg gcgagcgcgt aatggcgctg
     2821 attaatgaag ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc
     2881 ctgttgcatc gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc
     2941 gccattgagc tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt
     3001 tactatcaga cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg
     3061 ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc cacgtatgcc tctgcctgag
     3121 ccagaagtgc cacgccagtc ctttgcgccc gtcgcgccaa cggcagtaat gacgccaacc
     3181 caggtgccgc cgcaaccgca atcagcgccg cagcaggcac cgactgtacc gctcccggaa
     3241 accaccagcc aggtgctggc ggcgcgccag cagttgcagc gcgtgcaggg agcaaccaaa
     3301 gcaaaaaaga gtgaaccggc agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg
     3361 gaaagactgg cttcggtcac cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa
     3421 aaagcgccag ccaaaaaaga agcgtatcgc tggaaggcta ccactccagt gatgcagcaa
     3481 aaagaagtgg tcgccacgcc gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg
     3541 gaactggcgg cgaagctggc ggcagaagcc attgagcgcg acgcgtgggc ggcacaggtg
     3601 agtcaacttt cgctaccaaa actggtcgaa caggtggcct taaatgcctg gaaagaggag
     3661 agcgacaacg cagtatgtct gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt
     3721 gcacagcaaa aactggctga agcgttgagc acgttaaaag gttcaacggt tgaactgact
     3781 atcgttgaag atgataatcc cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac
     3841 gaagaaaaac ttgcgcaggc gcgcgagtcc attattgcgg ataataatat tcagaccctg
     3901 cgtcggttct tcgatgcgga gctggatgaa gaaagtatcc gccccatttg atcgtaagca
     3961 cagcttacgt tcgtcatcct taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg
     4021 tctgggtaac ctgatgaagc aagcccagca gatgcaagaa aaaatgcaga aaatgcagga
     4081 agagatcgcg cagctggaag tcaccggcga atctggcgca ggtctggtaa aagtgaccat
     4141 caacggtgca cacaactgcc gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa
     4201 agagatgctg gaagacctgg tggctgcagc attcaacgac gcagcacgtc gtattgaaga
     4261 aacgcagaaa gaaaaaatgg cctctgtatc ctccggaatg cagctgccgc ctggctttaa
     4321 gatgccgttc tgatgcaaac cagcccgctg ttaacacagc ttatggaagc actgcgctgt
     4381 ctgccgggcg ttggcccgaa gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat
     4441 cgtagcggcg ggatgcgtct ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac
     4501 tgcgccgatt gccgcacttt caccgaacag gaagtctgta acatctgttc gaatccgcgt
     4561 cgtcaggaaa acggtcaaat ctgtgtggtg gagagtccgg cggacatcta cgccattgag
     4621 cagacggggc agttttcagg ccgttatttt gtgttgatgg ggcacctgtc accgctggac
     4681 ggcatcggtc cggatgatat cggccttgat cgtctggaac agcgtctggc agaggaaaaa
     4741 atcactgaag tgatcctcgc caccaatccc acggttgaag gtgaagctac cgctaactac
     4801 attgccgagc tttgcgcgca atatgacgtg gaagccagcc gaatcgctca tggcgtaccg
     4861 gttggcggcg aactggaaat ggtcgatggc accacgctgt cacactccct tgccgggcgt
     4921 cataagattc gtttttaagc aaacgagagc aggatcacct gctctcgctt gaaattattc
     4981 tcccttgtcc ccatctctcc ctcatcctgt ttttaacctt aaaatggcat tattgaggta
     5041 gacctacatg aaaggacaag aaactcgtgg ttttcagtca gaagtgaaac agcttctgca
     5101 cctgatgatc cattctctct attccaataa agaaatcttc ctgcgtgagc ttatctctaa
     5161 cgcctccgat gcggcggaca agctgcgttt ccgtgcgcta tctaacccgg acctgtacga
     5221 aggtgatggc gaactgcgcg ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat
     5281 ctccgataac ggcgtgggga tgacccgcga cgaagtgatt gaccatctgg ggactatcgc
     5341 taaatccggt accaaatcat tcctcgaatc cctgggttct gaccaggcga aagacagcca
     5401 gctgatcggt cagtttggtg ttggtttcta ctctgcgttt atcgtggccg acaaagtgac
     5461 cgtgcgtact cgcgcggcag gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg
     5521 cgaaggtgaa tataccgttg ccgacatcac caaagaagat cgtggtactg aaatcaccct
     5581 gcatctgcgt gaaggcgaag acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag
     5641 caaatactcc gaccatatcg cgctgccggt agagctcgaa aaacgcgaag agaaagacgg
     5701 cgaaaccgtt atctcctggg agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa
     5761 gtcggaaatc accgatgaag agtacaaaga gttctacaaa cacatcgccc acgactttaa
     5821 tgatccgctg acctggagcc acaaccgcgt tgaaggtaag caggagtaca ccagcctgct
     5881 gtacatcccg tcccaggctc cgtgggatat gtggaaccgt gatcataaac acggcctgaa
     5941 actgtacgtc cagcgtgtgt tcatcatgga cgacgcagaa cagttcatgc cgaactatct
     6001 gcgcttcgtg cgtggtctga ttgactccag cgatctgccg ctgaacgttt cccgtgaaat
     6061 cctccaggac agcacggtaa cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca
     6121 aatgctggaa aaactggcga aagacgacgc ggacaaatac cagaccttct ggcaacagtt
     6181 tggcctggta ctgaaagaag gtccggcgga agatttcgct aaccaggaag cgatcgccaa
     6241 actgctgcgt tttgcttcta cttataccga ttcttctgcg cagaccgtgt ctctggaaga
     6301 ctacgtttcc cgcatgaaag aagggcagga gaaaatctac tacatcactg cagacagcta
     6361 tgcggcagcg aagagcagcc cgcacctgga actgctgcgt aagaaaggca tcgaagttct
     6421 gctgctttcc gaccgcatcg atgagtggat gatgaactat ctgactgagt tcgacggtaa
     6481 accgttccag tcggtgtcta aagttgacga gtcgctggaa aaactggctg acgaagttga
     6541 tgagagcgcg aaagaagcgg agaaagcact gactccgttc atcgaccgtg tgaaagccct
     6601 gctcggcgag cgcgtgaaag atgtccgtct gactcaccgt ctgaccgata cgccagcgat
     6661 cgtttcgacc gacgcggacg aaatgagcac tcagatggcg aaactgttcg ctgcggcggg
     6721 ccagaaagtg ccagaagtga aatacatctt cgaactgaac ccggatcacg tactggtgaa
     6781 acgtgcggca gatactgaag atgaagcgaa gttcagcgag tgggtagaac tgctgctgga
     6841 tcaggcgctg ctggcagaac gcggcacgct ggaagatccg aacctgttta ttcgtcgtat
     6901 gaaccagctg ctggtttcct gatgtcatgc cggatgacct ttgtgtcatc cggcattttt
     6961 cttttcatca tctgcacttt ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg
     7021 cctttcttga ggcaatcgcc tgttggtggt atcgtttatc gctttttcaa aaaattcgac
     7081 acattttaag gggattttcg caatgcgtat cattctgctt ggcgctccgg gcgcggggaa
     7141 agggactcag gctcagttca tcatggagaa atatggtatt ccgcaaatct ccactggcga
     7201 tatgctgcgt gctgcggtca aatctggctc cgagctgggt aaacaagcaa aagacattat
     7261 ggatgctggc aaattggtca ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc
     7321 tcaggaagac tgccgtaatg gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc
     7381 agacgcgatg aaagaagcgg gcatcaatgt tgattacgtt ctggaattcg acgtaccgga
     7441 cgaactgatt gttgatcgta tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta
     7501 tcacgttaaa ttcaatccgc cgaaagtaga aggcaaagac gacgttaccg gtgaagaact
     7561 gactacccgt aaagacgatc aggaagaaac cgtgcgtaaa cgtctggttg aataccatca
     7621 gatgactgca ccgctgatcg gctactactc caaagaagcg gaagcgggta acaccaaata
     7681 cgcgaaagtt gacggcacca agccggttgc tgaagttcgc gctgatctgg aaaaaatcct
     7741 cggctaattc gaaagcgcgc acggacagtc ccctcgcccc ctcggggaga gggttagggt
     7801 gaggggaaca ggcccgcaca agcaaactta tcagcaatct caggccggat attcattcgg
     7861 ccttttacaa aaacctcaat ctgcgctatt cctttttctg atttgacctc tcacaacaat
     7921 tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga taagaggcgg
     7981 taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc gatgccccca
     8041 cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc gtggttgata
     8101 cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg cgctcgccgc
     8161 gtgtggcgaa gatgtacgcc tcggtctgga tggaagacgg ctcgccgctg atggtttaca
     8221 gccgccagca acagcaggcg ttggcacaac gtttaccgga tacgcccgta gcgctgggca
     8281 tgagctacgg ctcgccatca ctggaaagcg ccgtcgatga actcctggca gagcatgtag
     8341 atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc ggtgcggtat
     8401 gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata tcgtttattc
     8461 gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta cgcgcttctt
     8521 ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt ccccagcgtt
     8581 atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc gaactggctt
     8641 ccgcactggg gatggcaccg gaaaaagtga tgatgacctt tcagtcgcgc tttggccggg
     8701 aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa aaaggcgtag
     8761 gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg ctggaagaga
     8821 ttgccgagca aaaccgtgag gtcttcctcg gtgccggcgg gaaaaaatat gaatatattc
     8881 cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt gccgcgtatc
     8941 gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg gcggttttca
     9001 tcattcgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg aactcacagg
     9061 gctgctgatg cgccgctaac gtctggtaaa gcagacggct gtcatccagc agcggatcga
     9121 actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta ttaaacagac
     9181 agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca tacatctgca
     9241 aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc acggaatccc
     9301 gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa tcgatctgtt
     9361 tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa tcaccggcaa
     9421 agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa taacaacaag
     9481 cagccacaat ttcctctatc gcttgcggaa aacgcgcttc aggtgaaagg gggtaatcaa
     9541 taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga tcgtgggtat
     9601 cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc gggctatctg
     9661 gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc atgtaagctc
     9721 tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg taatactgtc
     9781 gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccggctga agagtattca
     9841 caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt tccggcttca
     9901 tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa aatgcgatcc
     9961 cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg tgttaacatt
    10021 cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa cgtaaatcca
    10081 aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag ccagaaaacg
    10141 aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt gaagcgaaag
    10201 tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg gtgattgagg
    10261 atgacgtagc cgaagcgctt tatcaggagc taaaacagaa aaacctgatt acccatcagt
    10321 ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgcttgcg gacgaccgtt
    10381 cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc tatcgttacc
    10441 tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat ggcccgattg
    10501 gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc agtccaggcc
    10561 acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga gcctcggcac
    10621 tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg gaagcaacca
    10681 tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg ctgggcacca
    10741 agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac cacgtttcta
    10801 tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat ccgttgttgg
    10861 catctgacaa ggcgctggac tgggtagatc tggtgctgtg caccgccggg ccaatcggct
    10921 tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat ccgctgctgc
    10981 cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc cacaaggatt
    11041 gccagaatcc gctgcgtgta tattcgcaca ttgcgccgta catgggcggg ccggaaaaaa
    11101 tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat gacattaccg
    11161 ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc acctggttaa
    11221 cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat caggtactga
    11281 accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc ctggaagagt
    11341 cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt cacgccgcat
    11401 ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg cctacatatt
    11461 tttcatattt tacatccggc aaccaccgtt taccccgtca ccacctcacc cgccggtggc
    11521 gtttccagca gttccagcat ggtacgggcg atttcacgct cgcccatcac tacctgattc
    11581 gcaccacgtt cggtgatata cgccacttca tcgtcataat gggcgcgggc aataatctca
    11641 atatccggat ttttcgcgcg ggcagatgcc acaatctcac ccgcttcata accgttggga
    11701 atcgtcagga tcagccattt tgcacattcc agatgcgcca gttgcataat ttcttcgttc
    11761 gccgcattgc ccaatactgc gcggaccccg cgctctcgca gctcatcaac acgggttcgt
    11821 gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc cagcaggctg
    11881 cctacacgac cgtaacccac cagtagcgca tggttgcaaa tatccactgg gatctgcttc
    11941 tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt cgccagatat
    12001 ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc ccctgccagt
    12061 actaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc cgccaggata
    12121 aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt acgttgggag
    12181 tgaccaaaca gtcgcaccag gaaaaatgcg gctaacgact taccaaacag aataatcgcc
    12241 agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa caacatcccg
    12301 acggagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcagcgtatc gtgggcggca
    12361 cggtgactca gttcagactc gttcagtacc atcccggcaa agaacgcacc gagtgcaaag
    12421 gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc cagcaccgac
    12481 agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca cggcaccaga
    12541 cggcgaccta ccagcatcat aatggcgata aatgcgatca ctttgccgat ggtgatcccc
    12601 atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc tgccactgcg
    12661 ggcagcaaca ccagcgtcag aaccattacc aggtcttcca caatcaacca accgatggcg
    12721 atttgcccac gctgactgtc aattaattgc cgttcttcaa gtgcgcgcag taacaccacg
    12781 gtactggcgg tggaaagaca taaaccgaac acgataccgg tcattaacga ccagcccagc
    12841 acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat cgcgccggga
    12901 atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc gacgccgaac
    12961 atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc ggcaacaaag
    13021 cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag aggagaaata
    13081 cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc aacaatggtg
    13141 gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta ttgtccattt
    13201 ttggccggga aaaccaaaat tacaggtaat agtttatgac aatttcattg atgatgttca
    13261 tgaataatta ttgaattttg cagaaaaatg gaattagctg caaaaaaagc acggatcgga
    13321 aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga tgcagccaaa
    13381 gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag gaatatggtc
    13441 aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat actggtgtga
    13501 tcggcgataa gccccagaac tgccgctccc agacctccca taccaaaagc aaaaccgaaa
    13561 aagagtccag aaaccatacc gatacgtccc ggaagcagct cctgagcgta aaccagaatg
    13621 gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc cgtccagtgc
    13681 aggctggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga gccccaaatc
    13741 acatatttcc gcccaatttt atcccctaca ggcccgccga tcaccgtacc tgccgcaacg
    13801 gcaaacagga aggcaaacag atgaagctga gcattctgga tagataatcc gaatttttgc
    13861 atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt cgagaaaatg
    13921 aggattaaca gaatgctgac cgccagtacc actttgttgc gcggcagtgg attgataatc
    13981 gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg gctgatttgc
    14041 gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt gcctttgcca
    14101 taaggcgcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa gttgccgccg
    14161 acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg ggctacgcga
    14221 gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgccgc cagcagaact
    14281 gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa gcacatgcca
    14341 attggcaacg accacggcat cggatattta tcggtccagt agccgaccac tggttgcagt
    14401 agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa tgtcagagaa
    14461 aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat gtcgttcagc
    14521 agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt ggtcgttgac
    14581 gcagccgcgc ccgccacagg ctggggttgt tcactcattg ccataggaaa gtcacttttt
    14641 cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa catacctgta
    14701 tgcgtcgcct gaaggaagtc tcaacgccga atacagaatt tctaatctgg atgcagattt
    14761 atcttcaccg gacgcagact tgtctatgat gtcgcgtcat actatttttc aacacgttga
    14821 aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt agcgctgtta
    14881 accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa aacctacaaa
    14941 attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga atatggcgaa
    15001 tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt tgcggctgaa
    15061 ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc cgagtctgac
    15121 ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga cgcgatggcg
    15181 atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga aaagtgggcc
    15241 aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg cctgtttaaa
    15301 ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg tcattgggct gacaaccgat
    15361 gacacagcaa aaattggtaa cccggaatac ttcactgatg tcgaatttcg taagcccgcc
    15421 gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga cattattatc
    15481 gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc accgggcgat
    15541 gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg tggtcactcg
    15601 caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt gccgggtacg
    15661 ccatgcaagc cagatcaaca aaacggcatc tggattgtgc aggcgcatga gtggggcaaa
    15721 tacgtgggac gggctgattt tgagtttcgt aatggcaaaa tgaaaatggt taactaccag
    15781 ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag cgagcgcgtg
    15841 ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc accgttccag
    15901 aacaaaggca aagcgcagct ggaagtgaaa ataggcgaaa ccaatggtcg tctggaaggc
    15961 gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct ggcagcccaa
    16021 atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg tgattctatc
    16081 gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg caatgtggtg
    16141 gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt cgcgcagatg
    16201 aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc gaaagacggc
    16261 agactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac ttaccgtatg
    16321 gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga taacaaaccg
    16381 ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat ccagaaaagc
    16441 tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca gtaatccgaa
    16501 agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat cagcaaattt
    16561 ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc cgcgcttgcc
    16621 gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg ttggcagacg
    16681 ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac gctgcgcgac
    16741 catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt ttttaagatc
    16801 cagttgaccg gcgaccggcg taacggccac ggcaaggtgt ttcatatcac cgttcactgc
    16861 caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga cgacttcatc
    16921 gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg aaatcttgtt
    16981 tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa caaaaaaacg
    17041 aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta agagtattgg
    17101 caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga cggcataata
    17161 ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt tttttagcgc
    17221 aacatttgcg gtaaattccc ttctccatac aggtgtagtg caccgaccgc gaccacatat
    17281 cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg atgcatcagc
    17341 acatcgtaca gcgactgact gaacgtattg ggcagcgtta tttcattatt ttgcggcggt
    17401 gcattcagcc accagctcat catttgttgc agcaaccgtg cattggtgtg ccagtgggtc
    17461 agtgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat ggcaatctgg
    17521 ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc ctgcaatagc
    17581 tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt cgcctgaaga
    17641 accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat gcccatctcc
    17701 tggctaatgt gctgcagatt ttggagttgc tcctcgctaa tgcgctcttc cagcgcctcg
    17761 caggcaggca aattggcaaa aggcgtatcg ctggtggaaa catccgcctc gacgatcagc
    17821 gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat atcgtggcta
    17881 cccatatgaa tactgccaat cagatgaaaa tggcgattgc cgggaagggt gatatcgatg
    17941 gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac ccggtacaac
    18001 agatccatac gacctccctt tgtgaaatat catgctagcg cgcggggagg gatggtgcaa
    18061 atggagttgg ggacttgttt aatgtgtttg tatgattcag tatattcttg tatcgctatt
    18121 cacagggaag caacagttaa aaactatgaa acaggcaaca agaaaaccga cgacacccgg
    18181 agatattctt ctctatgaat atctggtacc gctcgatttg aaaatcaatg agttagcaga
    18241 gttgctgcat gttcatcgta atagcgtcag tgcactgatc aataacaatc gtaaactcac
    18301 cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt tttggctaaa
    18361 cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc aggaagaatt
    18421 gggacggatt gaaacagtgg ctgaatactt ggcacgccgt gaagagcgtg caaaaaaggt
    18481 cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttta cgccgcatcc
    18541 ggcagtcatg cgtcgatgcc aaatgtgcca ccctaaagca gcgcatccgc aatgacatac
    18601 ttattccttc ggtttaaacc gcagcaaccg gttggcgtta ctcactacgg taatcgacga
    18661 gagcgccatt gccgctccgg caactaccgg gttaagcagt gttccagtaa acggccacaa
    18721 aataccggcg gcgaccggaa taccgatact gttgtagata aaggcaccga gcaggttctg
    18781 cttcatgttg cgcagcgttg cgcgggaaat cgcgagcgca tccgcaacgc ccatcaggct
    18841 atggcgcatc agggtaatcg ccgcggtttc aatggcaaca tcactgccgc cccccatcgc
    18901 aataccgaca tccgcctggg ccagcgctgg cgcatcgtta atgccgtcgc ccaccattgc
    18961 cacctgacgt ccttcacttt gcaggcgttt aatcgcttcg gctttaccgt ccggcagcac
    19021 cccggcgatc acttcatcaa tcccggcttc tttggcgatc gcattggcgg tggttgggtt
    19081 atccccggtc aacatcacca gacgatatcc cgctttatgc aggcgttgca gcgccgccac
    19141 gctatcacta cgcaatggat cgcgtactgc cagcagggct accgctttcc cgtcaaccgc
    19201 cagcagcaca ggcgttgccc cttgcgatgc ctgagcagta atctccgctt cgatagcttt
    19261 ggtaccaacc tgttgctcat ttaacagcgc ctgattgccc agcaataacg catgaccttc
    19321 agcttcaccg ctcacgccca gcccgcgcaa tgtgcggaaa ccgttgacct gcggcagctg
    19381 catatcacct gctttatcga ggatcgcccg tgccagcgga tggctggaac cttgctccag
    19441 tgccgccgcc agacgcaatg cctgcgcttc atcaacatca gcaaacgttt tcactgcgac
    19501 aacctgcggc ttcccttcgg tcagcgtccc ggttttatcg aacacgacgg tatcgagtgt
    19561 actggcgcgt tgcagcgcgt cggcgtcccg caccagcacg ccaaactcag ccgcccgccc
    19621 gacgccggaa ataatcgaca tcggcgtcgc cagccccagc gcacacggac aggcgataat
    19681 cagtaccgtg gtggcaatca ccagggtata aacaatttgc ggtgccggac caaagaagta
    19741 ccagattgcc gcactgacca gcgcaatcac caccaccaca ggtacaaata cggctgagat
    19801 tttatccgcc agctgaccga tttctggctt gctgctctgg gcctggcgca ccatgcgaat
    19861 gatccgtgac agcgtggtat ggctgccaac cgcactggca cgaaacagca cgctaccgtc
    19921 ctgtaccact gtcccggcat ggacgctttc gccttcgcct ttttgctgcg ggattggttc
    19981 gcccgtcagc atcgcttcat ccagccatgc ttcgccctgg gtaatctcgc catctaccgg
    20041 cacgcgatcg ccggtcgtca ggcgcagcaa catacctggc tgcacttccg ccagaggcac
    20101 gcttttttca ccttcgtcag taaccaggcg cgccgtcggt ggggttaaat cgagtaattt
    20161 ctccagcgcc ttagaagaac gctgacgtgc gcgtgcttcc agcatatggc cgagattgat
    20221 cagaccgata atcatcgcgc tggcttcgta ataaagatgt cgcgcttcca tcgggaacca
    20281 ctgcggccac aggttgacgc tcatcgaata gagccacgcc acgccagtac ccagcgccac
    20341 cagcgtatcc atcgtcgctg caccgttcag caggcttttc catgcactgc ggtaaaaatg
    20401 gccgccggcg aaaaccatca ccgccagggt tatcaggccg ataaccaacc acaggctgcg
    20461 gttgtcagcg gtgaccatca tgttatcgcc aatcatcccc cagaccatca ccgggatacc
    20521 caccgccagt gcgacaattg cctgccagcg gaagcgcttc atcgtagcaa cggcggtttc
    20581 ctgctggcgc tcacggcgtt tcgcgtcatc ttcaatcgct tccgcgccgt agcccgcttt
    20641 ttccaccgcc tgcactaaat cttgtgggga ggcactgccc atcaccagcg cagtacgctc
    20701 cgccaggttt acccgtgcct gagtgacgcc cggtacgctt tgcagcgcat tttgtacgcg
    20761 ggtgacacag ctggcgcagc tcatgccgct cagcagcaac tgctggctgt catcgtcatc
    20821 ggcggtcgct gccggaagcg cctcagaaac cgctgtcagt gcttccgacg ggattgatga
    20881 ctccgccagc ggtttagcct ttgggtggct tacagatgcg tcataacccg cttgtttgat
    20941 ggtttcgatc agctgttctg cactggcagt cccggtaacg tgcgcttcag tgatagacac
    21001 atccgcttgt tccacatccg ggcgctgttc gagactttct ttcacgcgtt taacgcagtg
    21061 accgcaggac aggccgtcca gggtcaggtc gataatttgt gacataaaac actcctttaa
    21121 gacagttttg actggctgtg ataaaggtta aaccttccag caaggggaag gtcaagaaat
    21181 taataaacca ggcgagtaaa agtccgtaaa gattaaaaaa ttggctcaat ttgcatcagg
    21241 attagacatt tatctctttg ttttcctgta gttaagttgc gggtgctaag ttaaatcaag
    21301 atgcctgaaa atcggcaccg gggtgaggaa ttacctcccg catctataaa aaggagttaa
    21361 caaaagatgt tagatgcaaa caaattacag caggcagtgg atcaggctta cacccaattt
    21421 cactcactta acggcggaca aaatgccgac tacattccct ttctggcgaa tgtacctagt
    21481 caactggcag cagtggctat tgtgacctgc gatggcaaca tctatcgcgc gggtgatagt
    21541 gattaccgct ttgcgctgga atccatctcg aaagtctgta cgttagccct tgcgttagaa
    21601 gatgtcggcc cgcaggcggt acaggacaaa attggcgctg acccgaccgg attgcccttt
    21661 aactcagtta tcgccttaga gttgcatggc ggcaaaccac tgtcgccact ggtaaatgct
    21721 ggcgctattg ccaccaccag cctgattaac gctgaaaata ttgaacaacg ctggcagcga
    21781 attttacata tccaacagca actggctggt gagcaggtag cgctctctga tgaagtcaac
    21841 cagtcggaac aaacaaccaa cttccataac cgggccattg cctggctgct gtactccgcc
    21901 ggatatctct attgtgatgc aatggaagcc tgtgacgtgt acacccgtca gtgctccacg
    21961 ctcatcaata ctgttgaact ggcaacgctt ggcgcgacgc tggcggcggg tggtgtgaat
    22021 ccgctgacgc ataaacgcgt tcttcaggcc gacaacgtgc cgtacattct ggccgaaatg
    22081 atgatggaag ggctgtatgg tcgctccggt gactgggcgt atcgcgttgg tttaccgggc
    22141 aaaagcggtg taggtggcgg tattctggcg gtcgtccctg gcgtgatggg aattgccgcg
    22201 ttctcaccac cgctggacga agaaggcaac agtgttcgcg gtcaaaaaat ggtggcatcg
    22261 gtcgctaagc aactcggcta taacgtgttt aagggctgat catgatgaac acggaaggta
    22321 ataacggtaa caaacctctc ggtctatgga acgtcgtttc catcggcatt ggggcaatgg
    22381 tgggggcggg gatcttcgcg ctgctggggc aggctgcatt gctaatggaa gcctcgacct
    22441 gggtcgcctt tgcttttggc ggtattgtgg cgatgttttc cggttatgcc tatgcgcgtc
    22501 tgggggcgag ctatcccagc aatggcggca ttatcgactt ctttcgtcgc ggattaggca
    22561 acggcgtctt ttcgctggcg ctctcgttac tgtacctgtt gacgctggcg gtgagcatcg
    22621 ccatggtcgc ccgtgctttt ggcgcttatg ccgtgcagtt tttgcatgaa ggcagccagg
    22681 aggagcacct tattttgctc tacgcgttgg ggatcattgc ggtgatgacg cttttcaact
    22741 ccttaagcaa ccatgcggta gggcggctgg aagtgatcct cgtcggcatt aaaatgatga
    22801 tcctgttatt gctgattatt gccggtgtct ggtcgctgca accggcgcat atttccgtct
    22861 ctgcgccccc cagctccggt gcgttcttct cctgtatcgg gataaccttc cttgcctatg
    22921 cgggctttgg catgatggcg aacgcggcgg ataaagtgaa agatccgcag gtcattatgc
    22981 cgcgggcgtt tctggtggcg attggcgtta ccacgttgct ttatatctcg ctggcactgg
    23041 ttttgcttag cgatgtatcg gcattagagt tagaaaaata tgccgatacc gccgtagcgc
    23101 aggctgcttc tccgctgctc ggacatgtgg gttatgtgat cgtcgtcatc ggcgctttac
    23161 tggcgacggc ttcagccatt aacgcgaacc tgttcgccgt gtttaacatc atggacaaca
    23221 tgggcagcga acgcgaactg ccgaagctaa tgaataaatc cctgtggcgg cagagtacct
    23281 ggggcaacat cattgtcgtg gtgttgatta tgctgatgac ggcggcacta aatttaggct
    23341 cactcgccag cgttgccagc gccacctttt tgatttgcta tctggcggtg tttgtggtgg
    23401 cgatccgcct gcgtcatgat attcacgcct cgttgccgat tcttatcgtt ggtacgttgg
    23461 tgatgttgtt ggtgatcgtt ggctttatct acagtctgtg gtcccagggt agccgtgcgt
    23521 tgatatggat tattggctca ctcttactca gccttattgt ggcaatggtc atgaagcgca
    23581 ataaaaccgt ataacatctc tctgtgcgca gtacttcctg tattattgtg gtggcggtcg
    23641 atattcgcac tggcaaaaaa acgtgcttga atatctgttg aaacccttta acaaagcaca
    23701 ggaggcgttg cgcgaacgat gaacatcagc gatgtagcaa aaattaccgg cctgaccagc
    23761 aaagccattc gcttctatga agagaagggg ctggtgacgc cgccgatgcg cagcgaaaac
    23821 ggttatcgca cctacacgca gcagcatctc aacgaactga ccttactgcg ccaggcacgg
    23881 caggtgggct ttaacctgga agagagcggc gagctggtga atctgtttaa cgacccgcag
    23941 cggcacagcg ccgacgtcaa acggcgcacg ctggagaagg tggcggagat cgaacgacac
    24001 attgaggagc tgcaatccat gcgcgaccag ctgctggcac tggcgaatgc ctgccctggc
    24061 gatgacagcg ccgactgccc gattatcgaa aatctctccg gctgctgtca tcatcgggca
    24121 gggtgattaa gacgagacgg ctcggatatg tagggttatc ccttctatcg caatgacttc
    24181 aacatgcgta cctgcaccga gatcctcgct ggcgctgaca ggccatgaac tgtcaccgac
    24241 gcgcatatga ccgcgcccgt tgaccagcgg agattccagc acaaaacgtc ggccaatcag
    24301 ctgctgcccg cgctggttta aatgactatc gctgtgcttt tgttcgcgca cccgccgcga
    24361 caaccatttc caccacagcc aggcggcgag cagcgtcagg atggcaaaca ttaccccttg
    24421 ccactcccaa cccagcggca ccagccagac caccaggccg gtaatcaccg ctgccacgcc
    24481 gctccacaac aaataaccat ttccgcccag catctcggct gccagcagca aaccgccgag
    24541 actgagccag aaaatatgtg gatgaacgac cattaactcc atcatggctg agtccgtttg
    24601 ttggcgctgt ctttcaccag ctcggcaatc ccggcaatcg accccatcag gctgctggcc
    24661 tctaatggca tcatcactac tttgctgtta ctggaggaac cgatctgctg taacgcttcg
    24721 gtgtatttct gcgctacgaa gtagttcacc gcctgaatat caccggaggc gatagcttca
    24781 gacaccattt tggtggcgcg ggcttctgct tcggcggaac gttcacgcgc ttcagcctgt
    24841 aaaaacgccg actgacgttc gccttccgct ttcaggattt gcgactgttt ttcaccttcg
    24901 gctttgagga tttccgcctg acggatccct tccgcttcaa gaatgtaagc gcgtttggta
    24961 cgttccgctt tcatctgcgc gttcattgaa gagataagct ctgccggtgg gcgcacgtcg
    25021 cgaatttcaa tacgggtgac tttaatcccc cacgggttgg tggcttcatc gacaatacgc
    25081 agcaggcgtg agttgatgct gtcgcgctga gagagcattt cgtcaagttc cattgaaccc
    25141 aacacggtac ggatgttagt catggtcagg ttgatgatcg ccagctccag attgctgact
    25201 tcataagccg cgcgcggcgc gtcaatcacc tgaataaaac agactgcgtc gatggtaacg
    25261 ttggcgttat ctttcgagat aacttcctgg gaagggatat cgagcacttg ctccatcata
    25321 ttgatcttgc gaccaatgcg atccataaac ggcaccacca gactgagccc cggctgtaac
    25381 gttttggtat agcgaccaaa acgttctacc gtccattgat agccctgcgg cacgattttg
    25441 acgcccgcgc cgacaatgac cagcgcgaca aaaatgagaa tcgggataaa gataagcatc
    25501 ggaaaaacct cctgttgtac cgtccataat cagcaaaatt gctgcttgat taaacaaatt
    25561 atacctgatt actgaaagag agttccccct tattcctgcg aaggataaac tgtttttagt
    25621 aaaaatcaga aaaagggaac agtgatgcag gaaaatagtc ctttacttca gctacaaaac
    25681 gtaggatatc tggcgggtga tgcgaagatt cttaataaca tcaatttttc gctgcgtgct
    25741 ggcgaattta agttaattac cggtccttcc ggttgtggca aaagtacgct gctaaaaata
    25801 gttgcttcat tgatcagccc aaccaacgga acgttactgt ttgaaggtga ggatgtcagc
    25861 acactaaagc cagaaatcta ccgccagcaa gtctcttact gcgcccagac gccgacgctg
    25921 tttggcgata cggtatacga taatctgatc tttccctggc agatccgtaa ccagcagcct
    25981 gacccggcca tttttctcga ttttctcgaa cgcttcgctt tgccggacag cattttgacg
    26041 aaaaatatcg ccgagctatc tggtggtgaa aaacaacgca tctcactgat tcgtaacctg
    26101 caatttatgc cgaaggtttt attgctggat gaaatcacca gtgcgctgga tgaaagtaat
    26161 aaacataacg ttaatgagat gatccatcgt tatgtgcgcg agcaaaatat tgccgtgctg
    26221 tgggtgacac acgataaaga cgaaattaat catgcggata aagtgattac actgcaaccg
    26281 catgccggag aaatgcagga agcacgctat gaactcgcat aatattacta acgaatcatt
    26341 ggcactggca ttaatgctgg tggtggtggc aatcttaatt agccataaag aaaaactggc
    26401 gctggagaaa gatattttct ggagcgtcgg gcgagcgata attcagctga ttattgtcgg
    26461 ctatgtgctg aagtatattt tcagcgtgga tgatgccagc ctgacattat tgatggtgtt
    26521 atttatctgc tttaatgcgg cgtggaacgc gcaaaaacgc agtaaatata ttgctaaagc
    26581 ttttatctca tcgtttattg ccattacggt cggggcggga attaccctgg cggtgctgat
    26641 tctctctggg tcgattgaat ttatcccgat gcaggtgatc cctatcgccg ggatgattgc
    26701 cggtaacgcc atggtagcgg tggggttgtg ttacaacaat ttagggcaac gggtcattag
    26761 cgaacagcaa cagattcagg agaaactgag tcttggtgcg acgccgaagc aggcttcagc
    26821 gatattgatt cgcgacagta ttcgcgcggc tttaattccg acggtcgatt cagcaaaaac
    26881 ggttggctta gtgagtttac ctggaatgat gtccgggctg atatttgccg ggattgatcc
    26941 ggtgaaggcg attaaatatc agattatggt gacctttatg ctgctctcaa ccgccagctt
    27001 gtcgaccatt attgcctgct atttaaccta tcgtaagttt tataattcgc gccaccagtt
    27061 ggtggtgacg caattgaaga agaaatgatg cgatgccgga tgcgcagcat cgcatccggc
    27121 gttgtggttc atgtgccgat caacggaata tcaatacaac aatgcataca gctggcggcg
    27181 atacttcgac gccagtgcat cacccgtacc cagcgcagcg aggatctcct gaaacgtttt
    27241 acgcgtctga ccgtctgcgg cggtgagatc tttacgcaga tgcccgaaca gcaactccag
    27301 cgcctcttca ttgcgcccaa cctggtgcag ttgcagagcc agttgcgtcg ccagtgcggc
    27361 atcttctgga ttctccgcca cctgctgttg caactgttga atttccggcg tatcagccgc
    27421 ctgcttcagc agttcgattt gcgccaccag cccctggtag cgggtgtcct gatcctgcaa
    27481 cggaatggtt ttcagcaccg tttccgcatc ttcagaacgg ttcagcgcaa tcagcgtttc
    27541 tgccagcagc aggccgatct ccccgttctg attcgacaac tgccaggcgt ctttcagcaa
    27601 tggcagggca tcggtgtaat tgccttcctg catcagttgc atcgcctgct gcgctttcag
    27661 ctcttcttcg cgcggcagca ctttatccag cagggcgcgg atcgcctctt ccggttgcgg
    27721 cccctggaag ccatctaccg gttgcccgtt ctggaacaga tacacggtcg gaatcgcgcg
    27781 cagaccaaac tgggcggcaa tcatctgctc cgcgtcgcag tccagcttcg ccagaataaa
    27841 ttgcccgttg tactgcgccg cgaggctttc caggatcggg gttaactgca aacagtgctg
    27901 gctacgttca gaccaaaaat agaacagcac cggagtggtc atcgactgtt caagaacctg
    27961 ttgcaggtta gattcgttaa tgttgacaat attttctacg gacatggagt cgctctctgt
    28021 tgtcgatatt ttctttgaca tgggggctta agcgcgcgct tcaactcacc cctgcaatat
    28081 tttgtccatc acgcgccccg gcagcaggcg cttgagcacc attactgccc aggtcaccag
    28141 cgttaccgga tagcgcatct tcggcttctc gctaataaaa gcatggcgta ctttgtccac
    28201 caccgcttcc ggtcccaacg taaagcgggc ggcgatgccg ggattttcga ctggtttatc
    28261 actttgcgtc tggttgacgt tgtcggtgaa gcgagtacga atgggaccgg gttcgatcag
    28321 gctgacttta attccgctgt ggcgcagctc catgcgcagt gcgtctgacc acgcctccag
    28381 cgcatattta ctggccgcgt aagcgccacg acccggcgtg gagattaatc ccatcaccga
    28441 tgatgtcatc acaatacgcc cttcaccgtg cggtaacatc gcgggtaaca ggcgcatggt
    28501 gagctggtgt gcgccgaaaa agttggcgga aaactgctgt tccatctgcg cacggctgat
    28561 ggtggaaagg gggccataca tgccgaatcc ggcattgtta aagatcccat acagacaatt
    28621 atcggtcagg gcgatcacct cgtctgctgc gcgatcaaca ctttctggtg aatccagatc
    28681 gatcaacacg ccggtaaatc ccatgttgtt catacgctca acatcatccg gttttcggca
    28741 acctgccagc acatgaaaac cctggcgttt taattcgagc gcgctttcca ggccaattcc
    28801 actggaacat ccggtaatta agaccgattt ttgcataact ttacctgtca ggatctccgt
    28861 tgctttatga gtcatgattt actaaaggct gcaactgctt cgccatccag tcggcaataa
    28921 acggctgggc gtcgcggttg ggatgaatac cgtcatcctg catccattgt ggcttgaggt
    28981 agacctcttc cataaaaaag ggcagcagcg gaacatcaaa ctctttggcg agtttggggt
    29041 aaatggcgct aaaggcttca ttataacggc gaccatagtt tgcaggcaga cgtatttgca
    29101 ttaacaatgg ttcagcgttg gcggctttga catcctgcaa aatctggcgc agcgtttgct
    29161 cggtttgctg tggctgaaaa ccacgcaaac cgtcattgcc gcccagttca accagcaccc
    29221 agcgcggctg atgctgtttc agcagagccg gaaggcgcgc cagtccttgt tgcgaggtgt
    29281 cgccgctgat gctggcatta actaccgacg ttttactctg ccacttatca ttcaacaagg
    29341 caggccaggc cgcgctggca gacattcgat acccggcgct caggctatca cccagaatca
    29401 ataacgtgtc cgctgcggcg gcacggaagg ttaacaggac caggaacagg aagggcaaat
    29461 gccagcggaa aacattgttg aagttcatca tcttaagaag tccgtcggtc agggggagca
    29521 tgaactctcc atcctcaccg gagttgagct ggttgtcaaa cgtggcgaga ccatcgcact
    29581 ggtgggcgaa tcgggatcgg gtaagtcaac cttgctggcg atcctcgccg ggcttgatga
    29641 cggcagcagt ggcgaagtga gtctggtggg acaaccgcta cataatatgg acgaagaagc
    29701 gcgggcaaag ttgcgcgcga agcacgtcgg ctttgttttt cagtcattta tgttaattcc
    29761 tacccttaac gcgctggaaa atgtcgagct tccggctctg ctgcgcggtg agagtagcgc
    29821 ggaaagtcgt aacggggcga aagcgttgct cgaacagtta gggctgggta aacgtctgga
    29881 tcatcttccg gcacagcttt ccggcggtga acagcaacga gtggcgctgg cacgagcctt
    29941 taatggtcga cctgatgtgc tgtttgccga cgaacccacc ggcaaccttg accgccagac
    30001 gggcgataaa attgccgacc tgctgttttc cctcaaccgt gaacatggca ccacgttgat
    30061 tatggtgact cacgacctgc aactggcggc acgctgcgac cgctgcttac ggctggtgaa
    30121 cgggcagttg caggaggaag catgattgca cgttggttct ggcgcgaatg gcgctcgccg
    30181 tcgctattaa ttgtctggct ggcgctaagc ctggcggtgg cctgcgtgct ggcgctgggc
    30241 aatatcagcg atcgcatgga gaagggctta agccagcaaa gccgtgagtt tatggcgggc
    30301 gatcgggcgt tgcgcagttc gcgcgaagtg ccgcaagcat ggctggagga agcgcaaaag
    30361 cgcggcctga aagtcggcaa gcagctgact ttcgccacca tgacatttgc aggcgacaca
    30421 ccgcagctgg cgaacgtcaa agcggtggat gatatctacc cgatgtatgg cgatctgcaa
    30481 actaatcccc ctggcctgaa accgcaggcg ggcagcgtat tgctggcccc acgcctgatg
    30541 gcactgctta acctgaaaac gggcgacacc attgacgtgg gcgatgccac cttgcggatt
    30601 gccggagaag tgattcagga accggattcc ggttttaacc ccttccagat ggctccgcgt
    30661 ctgatgatga atctggcgga tgtcgataaa accggagccg tgcagccggg gagtcgggtc
    30721 acctggcgtt ataaattcgg cggcaacgag aaccagctcg acggctatga gaaatggttg
    30781 ttacctcagc ttaaacccga acaacgctgg tacggtctgg aacaggacga aggcgcgctg
    30841 gggcgatcga tggaacgctc gcaacagttc ctgctgcttt cggcgcttct gaccttgctg
    30901 ctggcagtgg cagcggtggc ggtagcgatg aatcattact gtcgcagtcg ctacgatctg
    30961 gtggcgatcc tcaaaacgct gggggcaggg cgagcgcaac tgcgtaagct aatcgtcggt
    31021 cagtggttga tggtgctggc gctttcagcc gttaccggtg gggcgatagg cctgttgttc
    31081 gaaaacgtgt tgatggtgct gctcaagccc gttctgcctg ctgcactacc gccagccagc
    31141 ctctggccgt ggctgtgggc gctgggtacc atgacggtca tctcgctgct ggtggggcta
    31201 cgaccgtacc gcctgttgct ggcaacgcag cctttacgcg tattacgtaa tgatgtggta
    31261 gcgaacgtct ggccgctgaa gttttatctg ccgattgtca gtgtggtggt tgtgctgctg
    31321 ctcgccggat taatgggtgg cagcatgctg ctttgggcgg tgctggcggg cgcggtagta
    31381 ctggctttgc tgtgcggtgt gctgggctgg atgctgctga atgtacttcg ccgcatgacg
    31441 ctgaaatcgc tacctctgcg cctggcggtt agccgcctgt tacgtcagcc gtggtcaaca
    31501 ttaagccagc tttcggcatt ttcgctctcc tttatgctgc tggcgctgtt gctggtattg
    31561 cgtggcgatc tgcttgaccg ctggcagcag cagctacctc cagaaagccc gaactacttt
    31621 ttaattaaca ttgccacaga acaggtagaa ccgctaaaag cgttcctcgc ggaacatcag
    31681 atagtcccgg aatcatttta tccggtagtg cgggcgcggc tgacggcgat taacgataag
    31741 ccgacagaag gtaatgaaga tgaggcgctt aaccgcgaac tcaatcttac ctggcaaaat
    31801 acgcgacccg atcataatcc gattgtcgcc ggtaactggc cgccaaaagc cgatgaagtg
    31861 tcgatggaag aggggctggc gaaacgctta aacgttgccc tcggtgatac cgtgactttt
    31921 atgggcgata cccaggagtt ccgcgctaaa gtgaccagcc tgcgcaaagt ggactgggaa
    31981 agtctgcggc ctaatttcta ttttattttc cctgaagggg cattagacgg gcaaccgcag
    32041 agctggctta ccagtttccg ctgggagaat ggcaacggca tgttgacaca actcaaccgc
    32101 cagttcccga ccattagcct gttagatatt ggcgcgattt taaaacaggt cggtcaggtg
    32161 ctggagcagg taagtcgggc gctggaggtg atggtggtgc tggtcaccgc ctgcggtatg
    32221 ttgctgttgc tggcacaggt gcaggtggga atgcgccagc gtcatcagga gctggtggta
    32281 tggcgcacac tcggtgcggg gaaaaaactg ctgcgcacga cattgtggtg tgagttcgcc
    32341 atgcttgggt ttgtttccgg cctggtggcc gcaattggtg cggaaacggc actggcagtg
    32401 ttgcaggcga aagtgtttga tttcccgtgg gagccagact ggcgattgtg gattgttctg
    32461 ccgtgcagcg gtgcgctgct gctgtcgctt ttcggcggct ggctgggtgc gcgactggtt
    32521 aagggtaagg cgctgttcag gcagtttgcg gggtgatgag agtaaataag gggcgttctg
    32581 gttgaatgga acgccttgtt taaattaatt ctacaatatg tttcattgat gatgttattg
    32641 aattggtgat ttcc
//
LOCUS       DAZGOB010000045        32630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGOB010000045 DAZGOB010000000
VERSION     DAZGOB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.045"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..177
                     /locus_tag="ACHLIF_002263"
     CDS             <1..177
                     /locus_tag="ACHLIF_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9606272.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
     gene            221..328
                     /locus_tag="ACHLIF_002264"
                     /pseudo
     CDS             221..328
                     /locus_tag="ACHLIF_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHLIF_002265"
     CDS             complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHLIF_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO9606273.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1169..1789
                     /gene="fklB"
                     /locus_tag="ACHLIF_002266"
     CDS             1169..1789
                     /gene="fklB"
                     /locus_tag="ACHLIF_002266"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9606274.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2098..3501
                     /gene="cycA"
                     /locus_tag="ACHLIF_002267"
                     /gene_synonym="dagA"
     CDS             2098..3501
                     /gene="cycA"
                     /locus_tag="ACHLIF_002267"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO9606275.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(3543..3641)
                     /locus_tag="ACHLIF_002268"
                     /pseudo
     CDS             complement(3543..3641)
                     /locus_tag="ACHLIF_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3768..4196
                     /locus_tag="ACHLIF_002269"
                     /pseudo
     CDS             3768..4196
                     /locus_tag="ACHLIF_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4301..5368
                     /locus_tag="ACHLIF_002270"
     CDS             4301..5368
                     /locus_tag="ACHLIF_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606276.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(5615..6277)
                     /gene="ytfE"
                     /locus_tag="ACHLIF_002271"
     CDS             complement(5615..6277)
                     /gene="ytfE"
                     /locus_tag="ACHLIF_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO9606277.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6385..7350)
                     /gene="ytfF"
                     /locus_tag="ACHLIF_002272"
     CDS             complement(6385..7350)
                     /gene="ytfF"
                     /locus_tag="ACHLIF_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9606278.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7458..8318)
                     /gene="qorB"
                     /locus_tag="ACHLIF_002273"
     CDS             complement(7458..8318)
                     /gene="qorB"
                     /locus_tag="ACHLIF_002273"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO9606279.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            8407..8787
                     /gene="ytfH"
                     /locus_tag="ACHLIF_002274"
     CDS             8407..8787
                     /gene="ytfH"
                     /locus_tag="ACHLIF_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO9606280.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8916..10859)
                     /gene="cpdB"
                     /locus_tag="ACHLIF_002275"
     CDS             complement(8916..10859)
                     /gene="cpdB"
                     /locus_tag="ACHLIF_002275"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO9606281.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            11049..11789
                     /gene="cysQ"
                     /locus_tag="ACHLIF_002276"
     CDS             11049..11789
                     /gene="cysQ"
                     /locus_tag="ACHLIF_002276"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO9606282.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(11779..12336)
                     /gene="ytfJ"
                     /locus_tag="ACHLIF_002277"
     CDS             complement(11779..12336)
                     /gene="ytfJ"
                     /locus_tag="ACHLIF_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO9606283.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            12661..12867
                     /gene="ytfK"
                     /locus_tag="ACHLIF_002278"
     CDS             12661..12867
                     /gene="ytfK"
                     /locus_tag="ACHLIF_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO9606284.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(12929..14272)
                     /gene="paeA"
                     /locus_tag="ACHLIF_002279"
     CDS             complement(12929..14272)
                     /gene="paeA"
                     /locus_tag="ACHLIF_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO9606285.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14595..15233)
                     /gene="msrA"
                     /locus_tag="ACHLIF_002280"
     CDS             complement(14595..15233)
                     /gene="msrA"
                     /locus_tag="ACHLIF_002280"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO9606286.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            15439..17172
                     /gene="tamA"
                     /locus_tag="ACHLIF_002281"
     CDS             15439..17172
                     /gene="tamA"
                     /locus_tag="ACHLIF_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO9606287.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            17169..20948
                     /gene="tamB"
                     /locus_tag="ACHLIF_002282"
     CDS             17169..20948
                     /gene="tamB"
                     /locus_tag="ACHLIF_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO9606288.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTVQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            20951..21292
                     /gene="ytfP"
                     /locus_tag="ACHLIF_002283"
     CDS             20951..21292
                     /gene="ytfP"
                     /locus_tag="ACHLIF_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO9606289.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            21504..21755
                     /gene="chpS"
                     /locus_tag="ACHLIF_002284"
     CDS             21504..21755
                     /gene="chpS"
                     /locus_tag="ACHLIF_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO9606290.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            21749..22099
                     /gene="chpB"
                     /locus_tag="ACHLIF_002285"
     CDS             21749..22099
                     /gene="chpB"
                     /locus_tag="ACHLIF_002285"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO9606291.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMYLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(22179..22709)
                     /gene="ppa"
                     /locus_tag="ACHLIF_002286"
     CDS             complement(22179..22709)
                     /gene="ppa"
                     /locus_tag="ACHLIF_002286"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO9606292.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            23019..23975
                     /gene="ytfQ"
                     /locus_tag="ACHLIF_002287"
     CDS             23019..23975
                     /gene="ytfQ"
                     /locus_tag="ACHLIF_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO9606293.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            24115..25617
                     /gene="ytfR"
                     /locus_tag="ACHLIF_002288"
     CDS             24115..25617
                     /gene="ytfR"
                     /locus_tag="ACHLIF_002288"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO9606294.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            25631..26653
                     /gene="ytfT"
                     /locus_tag="ACHLIF_002289"
     CDS             25631..26653
                     /gene="ytfT"
                     /locus_tag="ACHLIF_002289"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO9606295.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            26640..27635
                     /gene="yjfF"
                     /locus_tag="ACHLIF_002290"
     CDS             26640..27635
                     /gene="yjfF"
                     /locus_tag="ACHLIF_002290"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO9606296.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(27668..28666)
                     /gene="fbp"
                     /locus_tag="ACHLIF_002291"
     CDS             complement(27668..28666)
                     /gene="fbp"
                     /locus_tag="ACHLIF_002291"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO9606297.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            28842..30215
                     /gene="mpl"
                     /locus_tag="ACHLIF_002292"
     CDS             28842..30215
                     /gene="mpl"
                     /locus_tag="ACHLIF_002292"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO9606298.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(30371..30922)
                     /gene="yjgA"
                     /locus_tag="ACHLIF_002293"
     CDS             complement(30371..30922)
                     /gene="yjgA"
                     /locus_tag="ACHLIF_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO9606299.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWHDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            31016..32368
                     /gene="pmbA"
                     /locus_tag="ACHLIF_002294"
     CDS             31016..32368
                     /gene="pmbA"
                     /locus_tag="ACHLIF_002294"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO9606300.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            32551..>32630
                     /locus_tag="ACHLIF_002295"
     CDS             32551..>32630
                     /locus_tag="ACHLIF_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="electron transport protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO9606301.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLE"
BASE COUNT         7729 a         8134 c         8908 g         7859 t
ORIGIN      
        1 cgcctgtcac aaagtaaatt cagatgccag gtatgtggat atacagcgaa cgccgatgta
       61 aacggcgctc gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agagatggtg
      121 cagtcaggcc gctcgttgaa gcaggaaccc accgaaatga ttcaggcgac agcctgaacg
      181 aagcagggat ccacgtcctt cagggcgtgg aggatgtcaa catatcagta tggtttcccc
      241 ccaggcactg gaagccagac gtaaggtcat caaagagaca atccttagct ggcttttggt
      301 tgacccttca tctaccgcgc acgaataaaa ctgccatccg gctggcgggt aaacaggacc
      361 tgttgattat tccccgtatc aatggttaag cccgtcacca cgccgctggc gttctggcgg
      421 attttcacca tttgcccgtt ttgcaaatta ctcagcggct tacccgcgcc ttctacctgc
      481 gccattgcat agacatcggt agcgggcagt ccgtggtcgc ggaacagctg cgccatcgtt
      541 ttaccgggtt cgacacggta agagcgccat tgattatcaa ttccgctatc tggctggaat
      601 ggttgcgttt gggtagtttg cggctgctct tctggctggc cttcctgtat aggttctgga
      661 gcgaccggag ccacctgatc aggatcattt tgtggggtga ctaattgcgc ccgcagctgt
      721 tcttctgtcg gcggttgtga ttgcgactga atatctaact gcgcttcacg cgtcaccacc
      781 ggcgcgttgg gcgtatcatc agatggaagc agaaatccga ccaccaatac aatggcggca
      841 ataatgatcc cccgacgatg catcggcggc agcgggtcca tgaagcgaaa attgtccggc
      901 gcgtgccaga ctttctccag ggttggtttt agttcaaagc gcccgggcat ggtttcctcc
      961 tgctccgcgt cttgttcctc aatcatagcc tatgaataag ctaacgctat gatgtccgtg
     1021 gtaaacccgc ctttattata ttcatacggg atattgctat tgtttctttt tccccgggat
     1081 ttgtcatcat tcctgcgaca aagttttacc caaagaagtg tggctgatat gctgcccgct
     1141 acttttatac cctaagaaag gaaatacgat gaccacccca acttttgaca ccatcgaagc
     1201 gcaagcaagc tacggcattg gtttgcaggt agggcaacaa ctgagtgaat ctggcctgga
     1261 agggctgctg ccagaagcac tggttgcagg tattgccgat gcgctggaag gcaaacatcc
     1321 ggctgttccg gttgatgtgg tgcatcgcgc gctgcgtgaa atccacgagc gcgccgatgc
     1381 cgttcgtcgt cagcgtttcc aggcgatggc tgctgaaggt gtgaaatacc tggaagaaaa
     1441 cgccaaaaaa gaaggtgtga atagcaccga atctggcctg caattccgcg tgatcaacca
     1501 gggtgaaggc gcaattccgg cacgtaccga ccgcgttcgt gttcattaca ccggtaaact
     1561 gatcgacggc accgtgtttg acagctccgt tgctcgtggt gaacccgctg aatttccggt
     1621 taatggcgtg atccctggct ggattgaagc actgactctg atgccggtag gttctaaatg
     1681 ggaactgact atcccgcagg aactggcata tggcgagcgc ggcgcaggcg catccatccc
     1741 tccgttcagc accctggtgt ttgaagtcga actgctggaa atcctctaag cagcgcattc
     1801 tgttcccctc gaaccagagg ggagcaggca ttcagcaata aacccttcag tttgccaaac
     1861 ggcgctattt tgtgttgcaa agaccccgta agcgtgtatt tttgtgagct gtttcgcgtt
     1921 atcaccgtga tatgacactc actttaaaca taaaattaac attagatcta aatcttagta
     1981 ttcatcccgc gtattgttac ctaatatcga tgagtcccga tacagattcg tcgtatcata
     2041 ggctgactaa aggccgtaga gcctgaacaa cacagacagg tacaggaaga aaaaaacatg
     2101 gtagatcagg taaaagtcgt tgccgatgat caggctccgg ctgaacagtc gctacggcgc
     2161 aatctcacaa accgacatat tcagcttatt gccattggcg gtgccattgg tacggggttg
     2221 tttatggggt ctggcaaaac gattagcctt gccgggccgt cgatcatttt cgtttatatg
     2281 atcatcggtt ttatgctctt tttcgtgatg cgggcaatgg gggaattgct gctttcgaat
     2341 ctggaataca aatcttttag tgacttcgct tccgatttac tcgggccgtg ggcaggatat
     2401 ttcaccggct ggacttactg gttctgctgg gttgtaaccg gtatggcaga cgtggtggcg
     2461 atcacggctt atgctcagtt ctggttcccc gatctctccg actgggtcgc ctcgctggcg
     2521 gtgatagtgc tgctgctgac gctcaatctc gccaccgtga aaatgttcgg tgagatggag
     2581 ttctggtttg cgatgatcaa aatcgtcgcc attgtgtcgc tgattgtcgt cggcctggtt
     2641 atggtggcga tgcactttca gtcaccgact ggtgtggaag cgtcattcgc gcatttgtgg
     2701 aatgatggcg gctggttccc gaaaggttta agtggcttct ttgccggatt ccagatagcg
     2761 gttttcgctt tcgtagggat tgagctggta ggtacaaccg ctgcggaaac caaagatccg
     2821 gagaaatcac tgccgcgtgc aattaactct attccgatcc gtatcattat gttctacgtc
     2881 ttcgcgctga ttgtaattat gtccgtgacg ccgtggagtt cggtagtccc ggagaaaagc
     2941 ccgtttgttg aactgttcgt gttggtgggt ttgcctgcgg ctgccagcgt gatcaacttt
     3001 gtggtgctga cctctgcggc gtcttccgct aacagcggcg tcttctctac cagccgtatg
     3061 ctgtttggcc tggcccagga aggtgttgca ccgaaagcgt tcgctaaact ctctaagcgc
     3121 gcagtacccg cgaaagggct gaccttctcc tgcatctgtc tgctcggcgg cgtggtgatg
     3181 ctgtatgtga atcccagcgt gattggcgcg ttcacgatga tcacgacggt ttccgcgatt
     3241 ctgtttatgt tcgtctggac gattatcctt tgctcgtacc tggtgtatcg caaacagcgt
     3301 cctcatctgc atgagaagtc gatctacaag atgccgctcg gcaagctgat gtgctgggta
     3361 tgtatggcgt tctttgtgtt cgtgatcgtg ctgctgacac tggaagatga cactcgccag
     3421 gcgctgctgg ttaccccgct gtggtttatc gcgctggggt tgggctggct gtttattggt
     3481 aaaaaacgca tggcgaagta atgccgctaa ggcacctgcg ggtgtctttc aggctgcaaa
     3541 attgcctgat gcgctacgct tatcaggcct acagaacctc tgcaatatat tgagtttgcg
     3601 tgcttttgta ggccgggtaa ggcgtttacg ccgcatccgg catgaacaaa gcacactttg
     3661 tcagcaatct cctttgtctt tatacattcc taaaaatact tcccgtcatc gacagctctc
     3721 aggcgcgggc ttaccgtgga tctttgccac tcattgcaga gttcactatg gctaagcaca
     3781 gttggttatt aatcgccgca ttacctctct ccatttcacc ttcctggggc gcagattttt
     3841 attaccgcca gcaggagaaa ggcacggttt atgttgccga acagaaggga gaaaaggatg
     3901 agatcctctc cgaactgccg ggcgttaatt tttcccgcct ctggcgtatt gccaatttag
     3961 ccaataacca agaaacccgc ttactgtccg attttaatcc cgataagttc gattgcgatg
     4021 atcggaataa ttgccagcat acctggctca ccgatggacg ctctgttctt tggtctggca
     4081 aagtcctgaa aaatccgccc ggtgaaccta atgtcgatgc cgccagtttt caggcattcg
     4141 gtgctttcgc tgccgataaa cgcagcgtct attttgacgg tcagcgtacc gatgatagat
     4201 gaagtggata tgtatatagc tgtgtctcag ttgcaaacaa atatttatgt accgaaataa
     4261 aagtatcaat agacaattaa ttaataaatc aggagataaa gtggctatat tacatccaca
     4321 ggagtgctgg ttgctggaaa gaataatgtc gccggaatat tatcggcgtc gttttgaggg
     4381 ctggcaggag tttgtcgagc tgtgcgagcg acaggtggca gagtggagca agaccatgcc
     4441 gctggatgtg cgccgtcgtc cgctgtgcga gcagattgac gtcgtctggg gcgggcgagt
     4501 gttgcccaat atccgcagta ctttaaagtc ggtacagtat gactttattc agctacagca
     4561 gggggacctg cgggtactgc aatccggggg caatattagc agtgatatga aaggactaat
     4621 tgattatccg tcggactgga tgtcactcgc cgcgcaaaaa cagtatgaca gactgaaatg
     4681 gcgaggtgca cattataata atttaatcag acgtacctcg ggcggatact ggtatgacgg
     4741 cgaactgact tattattatg aggaaagtct gcacggcccg ctggccctgc cgatgcaatt
     4801 accgttgtat gagctggata gtagcgtata cctgcgtgag gatgactcgg taacggtggc
     4861 tggcctgtat ctgccggata tcccagatgc cagcgcccaa ctgttgtacc ggagtgaaca
     4921 tattcccgag gcttggcagg ggcgggtacg aacgaagtat gtcaacgagg ctgggataca
     4981 agaatattac tgggaaagcg gggcgtgggc aaaatgcaat tggaaacgta tacggcgggt
     5041 ggcaaaccga tttatcaatg tgccgccgga gggatttttc ccacagggca tgccggagga
     5101 gttgtacaac tggccacagc gcgaagcgca gtatgttaca gaccggcagc ggatagcggc
     5161 ctttagcggt gaagcttgtc ctcatagcgg ggagtggtcg atttttgtag aaggtagaca
     5221 agcgacggtg acactggagc agggagagca gatgccagaa tggacggaca ggaaaatgga
     5281 gggggagtat aaaagaggcg agaaattcca tgtgctgtgg tcgctgatga accgccatga
     5341 tggcgggtcg gtgtgggtgg aagcgtagcg gtagctggat gtacacgtaa aaaccaggga
     5401 atgggtgatt ttattctggc ttatacagga gcgatgaata agtaattata ttaatgaaaa
     5461 aaataagtta gtaaccgttg aaggaactta ttattggctg aataatgaat tttcccagtt
     5521 cagtagatac aaaagattgt cccggagtgg tgaataactc aaagcatttg aaaacgcacc
     5581 tgattgtgta aaccaggtgc gttgatcgaa caaattactc acccgccagc gcgcgtggga
     5641 acagtacatt gttttccagg ctgatgtgat ccatcaggtc atcaatcagt tcattaatac
     5701 cgttatacat cgctttccag gtggtgcagg cttccggcgg cggtgtgacg ttattggtgg
     5761 tgtgtttaat cacttccagc agttcgcccg cttcatcgtg ctcgctttcc atcacgctga
     5821 ttggtcccat tgcctggctg cccatgcctt gtttgatcat cgggaagagg atctgctctt
     5881 ctttcatcat gtggctggaa agctcttcat gcagcatagt caggtatttt gtcagccctt
     5941 ttggcacgct cggtttgtcg gcgtgaacgc gctcgacttt agtcgcttgc aggatcagct
     6001 ccggcagctg ctcgcggtga cgatcgtggt agcgcacgat gatatggtca atgatttctg
     6061 ccagcggggc gctacgccag tctttctcaa tcggttgttc agcaagcttt gccagttcag
     6121 cttcaatgac ctcaacatcc agttctttac gtgccgccgc gcgcgccagc gtctgcttac
     6181 cgccacagca gtaatccata tcatatttac gaaacagagc cgaagcgcga gggatagaga
     6241 gcgccagttc acctaaaggt tggtcgcgat aagccatagc tgatacctca ttcttaataa
     6301 gataagatgt attttaaatg catctttaag gcaaaagcta taacccttac gtagcagagg
     6361 cttatttaac tcactgccag cgtgtcaact ttctgagaca gattcagtta aaggtttttc
     6421 tggttttgct ctgaccgcaa tgaccacgcc aatcactaac agcgcgatac cgctcagcgt
     6481 cattagcggc ggcatttgct ggcggagtaa aaaggtgtac agcaaacctg ccagcgtttc
     6541 gaaaacaatc agcggcccga gaatcactgt cggtaatcgc tggctggcga cgttccagca
     6601 gagtgcgcca acccatgagc aaagcacggc tatcgcaacc atcagactaa taaacaccag
     6661 cggacggggg ccaaaaggta aggagaagtc cggcgtttgt atattcagcc agtaacaggc
     6721 gacgagatag ccgatgagag aaaccggcag cgtgaccagc gcctgcgccg tcgcccacat
     6781 catcggatgt ttgtcgggat tttcccgcag ccagcgggcg ttgcgcaggg cataccatgc
     6841 ccagcagacc acggaaacta acgctagcac gatgccagag gtataacgtg cccagtcaaa
     6901 atcggggagt ccgtggttta actcagcaat attcacactc gccaggccga tgccaataca
     6961 aatcagtgcc ggggcgagtt ttccccacgc gagtttgccg tcgcgctggc tataaagcag
     7021 attggcaaag acaggaatga ccaccggcag ggtgccgata atcatcgtgg aaacaggcgc
     7081 gccagtacgt tgaatggcac tggcaaggca gaagtaataa atgaggttgc ccatcatagt
     7141 gagcatcaag gcggtaagcc agtcccgacg cgccaactga cgcagacgca cgcgtcccag
     7201 ccaggcaatg ggcagcgcaa ttaaccctaa cgccagataa cgccccatcg actgcaacat
     7261 cgccgggtat tccggtacga tcaacggccc gacaaaaata agcccccaca tcaaccctgc
     7321 taacagggcg tacagcacgc cgctaatcat tactggcatc cattggtctg tcagaagaaa
     7381 gttcaggaag cagacatgat tgctcccggc atcccgtcat tattagagag gatgcgggag
     7441 gatgccactt taattaacta gttattaaca ttaaaaagat ggcttacgct ttcggctaac
     7501 gttgtcgttg ggcggccaat caatttgcta agcgttttgc tgttatcaaa cagaccacct
     7561 ttcgatgcgc caacgtcaga atccgccagc atatccgcca gtccgtcggg cagtccgacg
     7621 cttttcagtg ccgcggcgaa atcggcttcg ctcagatttt gataggtaac ctgtttgccg
     7681 ctctgtttgg tcagctctgc cgctaactgt gtcaatgtcc aggcactatc gcccgccagt
     7741 tcgtaaacct tgccttcgtg accggcttcg ctaatcacgc gtgccgcagc tgccgcataa
     7801 tctgcccgcg ttgctgaggc gattttgcca tcgcccgccg caccgataaa tacgccgtgt
     7861 tccagtgctg ccggggcgct ggcgaggtag ttttcgctgt accagccgtt gcgcagcagg
     7921 gtgtaaacga tgccagaatc agccaacatt ttctccgtct cgatgtgctc atcggcgagg
     7981 ccgagcgggg aggtatctgc atgtagcagg ctggtataag cgataaattt cacaccagcc
     8041 gcctttgcgg cattaataac attacgatgc tgcggggcac gttgacccac ttcgctggaa
     8101 gagatcagca gtagtttttc cactccctga agtgcagatg tcagtgcggc ttcatcgccg
     8161 tagtcagcct gacgcacggt aatgccttgt gctgccagag cctgggcttt tgccggatta
     8221 cgaacgatag ccactatttg gctgacagga accgttttct tcaaggattc aataacatag
     8281 tgaccaagtt ggccagtggc accagtaata gcgatcatgg gaagtctcca tcgttttgtc
     8341 ttgtgtttaa atacgctaac acctaaactt acttttagta agtacgcaca aaaaggtaag
     8401 tatgaaatga gtcaggttag cctgtcgcaa caactgaaag agggtaatct ctttgcggaa
     8461 cagtgcccgt cgcgcgaggt gttgaaacac gtcaccagcc gttggggggt gttgattctg
     8521 gtggcgctac gcgaaggtac tcatcgcttt agcgacctgc ggcgcaaaat cggcggggtg
     8581 agcgaaaaga tgcttgcgca gtcgttacag gcgttagaac aggatggttt tcttaaccgt
     8641 atcgcgtatc cggtggtgcc gccgcatgtg gaatatagcc tcacgccgct gggcgagcag
     8701 gtgagcgaaa aggttgctgc actggcggac tggattgagt tgaatttgcc cgaggtgttg
     8761 gcggtgcggg atgaacgtgc ggcataactg ccggatgcgc tgagcttatc tggtctacgg
     8821 tgcatggctt gtagggcgga taagatgcgc cagcatcgca tccgccaata aatgccggat
     8881 gcggttgatg atttataggc cgaaaaagaa gtgttttact tactcaaatc cacctgataa
     8941 atcgcaaacc cgatatcatc ggtcgcgact ttattcatcg gatattgccc tttctcttta
     9001 ataaacgctg cggctttatc tgacggagaa gtttcgaaac ggatatccag tttcttatcg
     9061 ccagctatcg gtgctaaacg ccagttgtta tctgccgccg ggtgaatttc ccccgcacgt
     9121 ttcgactcat cagcaatcca cgctgccagc accgagcggt tctcatccgg tgaagcaaaa
     9181 gcgatatggc tgtcgcccgt accggcaaat ttgccgccgt aagcgcgata gttattggtg
     9241 gcaacgagga acatggcgtt cggatcaatc ggcttgccat taaaggtcag gttcttaatc
     9301 ctttccgcat tggcattaat catctggcac tcgccgtcat aacgggcagg ctgggtaaca
     9361 tcaatctgat aattcacacc atcaatcaca tcaaagttat aggtgcggaa accatcccag
     9421 ttgatgagtg attgcggttt cgtgctgtta ggatcaatct ggttaaactg tccggcggag
     9481 cactccagcc actctttcac ctctttaccg ctggctttca ccacaatcag cgtattgggg
     9541 tagagataaa gatcggcggc attacggaag gtcaactggc ctttttccac ctccacatag
     9601 cttgccgggt catttttgcg accaccaact ttaaacggtg cggcagctga aagcaccggc
     9661 agttttgcca gatccggatc gccctgaatg taatgttcga catacgcttt ttgcgcgttg
     9721 ttcactactt gcacggtcgg atcgtcctgc accagcgcca gatagctata catattgtcg
     9781 gcagatttac cgattggctt gctgacgaac tggcgtgtgg catcgtgatc ggctttgagt
     9841 gtttctacca gcttgctgtc ttccgccgcg agggattttt tattggcgat gtcgtaaatc
     9901 ggccgagctt ccgctttcgc ctgcgtcacc tgccatttac cgctgtcatt actgagttgt
     9961 aagtcgacca ccccaagatg atcgccccac atgcccggca ttaccgccgg aacaccattc
    10021 agcgtgcctt tggtgatatc agccccttcg atatcagcaa aatctttacc cgggaaaacg
    10081 gcgtgagcat gaccaaacat aatggcgtta acgcccggaa tttcactgag gtaataaact
    10141 gagttttccg ccattacttt atacggatcg gcagacagcc cggaatgcgc cagaacgaca
    10201 acgacatcgg cacctttctc gcgcatttca ggcacgtatt tgcgcacggt ttcggtaata
    10261 tcattcaccg tcacttttcc ggataaatta gctttatccc agcccatgat ttgcggcggc
    10321 acgacgccaa tatagccaat cttcagcgtc tgtttttttc cgtctttatc gaccacttcg
    10381 gtatctttaa ttaaatacgg tgtaaacatt ggctgtttgg ttctggcgtc aatgacgttg
    10441 gcatttacat aagggaattt cgctcccgcc agcgcatttt tcaggtaatc cagaccgtag
    10501 ttaaattcat gattgccgag tgtaccgacc gtataatcca gcgtattcag cgccttataa
    10561 accggatgaa catcacctgc ttttaatcct ttcgccgaca tgtaatcggc cagcggactc
    10621 ccctgaatca aatcgccgtt atcgaccagt acgctgtttt tcacttcatt gcgggcatcg
    10681 ttaatcaggc ttgccgtacg taccagtccg aatttttccg tggcggtgtc tttgtaataa
    10741 tcgaaatcca tcatgttgct atgcagatca gtggtttcca tgatacgcag atcgaccgtc
    10801 gctgcattca cactggcggc aatcagcgtg gccaggagcg ttgcgctaaa cttaatcatc
    10861 agggacatcc ttttatcatc gggaatacga aagaaaaggg agaataaacg tcttacttat
    10921 agaacagtga agaatgccac aattttacgc tttgaaaatg atgacactat cacagttggc
    10981 gcattcatta acgatagggt ataagtaaaa caataagtta acaccgctca cagagacgag
    11041 gtggagaaat gttagatcaa gtatgccagc ttgcacggaa tgcaggcgat gccattatgc
    11101 aggtctacga cgggacgaaa ccgatggacg tcgtcagcaa agcggacaat tctccggtaa
    11161 cggcagcgga tattgccgct cacaccgtta tcatggacgg tttacgtacg ctgacaccgg
    11221 atattccggt cctttctgaa gaagatcctc ccggttggga agtccgtcag cactggcagc
    11281 gttactggct ggtagacccg ctggatggta ctaaagagtt tattaaacgc aatggcgaat
    11341 tcaccgttaa cattgcgctc attgaccatg gcaaaccgat tttaggtgtg gtgtatgcgc
    11401 cggtaatgaa cgtaatgtac agcgcggcag aaggcaaggc ctggaaagaa gagtgcggtg
    11461 tgcgcaagca gattcaggtc cgcgatgcgc gcccgccgct ggtggttatc agccgttccc
    11521 atgcagatgc ggagctgaaa gagtatctgc aacagcttgg cgaacatcag accacgtcca
    11581 tcggctcttc gctgaaattc tgcctggtgg cggaaggaca ggcgcagctg tacccgcgct
    11641 tcggaccaac gaatatttgg gacaccgccg ctggacatgc tgtggccgca gctgccggag
    11701 cgcacgttca cgactggcag ggtaaaccgc tggattacac tccgcgcgag tcgttcctga
    11761 atcctgggtt cagagtgtct atttattaat taatttatgc agcaggtcca tcacttgctg
    11821 cacctcttcc tgagtaagcg ccccgtcttt ggcccattgc acgcgaccgt ctttatccag
    11881 caccactaca gcggaacttt tttcatccag ctgccaggca ccgcgtgcga cgccattgct
    11941 atcaacaata aactgcgacc agggataaag ctttttatta ctctccagac tgctgcgcac
    12001 aaacatgccg gaacccggaa ttgcgtcgtc ggtgttaaca atggtggtgg tctggtaacg
    12061 atcgtgcggt aacttcgctg atttaatcgc ttcaatcagc gtcgcgtttt tctcttttgc
    12121 agaggtgcga ccagcaatat gttgcagtac tcgcactttt cccactaact gcgcgctgtt
    12181 ccaggttttg tagctaaact gatctttatc aagcaccaac tcgccacgat cggtgatgcc
    12241 aatcggcggc actcgctgac cggtttcgaa ctgatgtgcg gaagccatca tcggcaacag
    12301 caggcaggtg agtgccagaa tcttgcgtag ggtcatggtg tttccttctt atgatatgca
    12361 ggtgatccga ccacttgggt ctgagtttta atcatatgtg ctatttatcg aatttcccgc
    12421 aagtgtgatg ccagtttgcg gtcaagcgca caaatcatat gaaaaatgaa tgcttatact
    12481 gaagaccgcg cttcggtaaa aagataattc tgaataattg taacctttag gtaaaaaaag
    12541 ttatacgcgg tggaaacatt gcccggatag tctatagtca ctaggcatta aaatttgcgc
    12601 ctcataatag ttgggccgat tgtggcaccg cacaggcgta atactcagca ggagataaca
    12661 atgaaaattt tccaacggta caacccactt caggtggcga agtacgtaaa gatcctgttc
    12721 cgtggacggt tatacatcaa ggacgttggc gcttttgaat ttgataaggg taagattctt
    12781 atcccaaaag tgaaggataa gctgcatttg tcagtgatgt ccgaagttaa ccgtcaggtt
    12841 atgcgtctgc aaacagagat ggcttaacca aagtgctatg cagtaataag acggctcctg
    12901 attcaggagc cgttgatgtt tctgggggtt acgcgacgct ttcttcttta tctttagcgt
    12961 caggcagttt tggcgaaagg gcggtggcct tgctgtcgat ccgggtcacc agcagctggt
    13021 cgatgcggta gttatcgata tccaccactt caaatttgta gccggcgaat ttcaccgaat
    13081 cggtgcgttt cgggatctta cgcagcataa acatcataaa gcctccgatg gtttcgtagt
    13141 tgcccgactg cgggaactcg tcaatatcca gcacgcgcat gacgtcgtca attggggtgc
    13201 cgccgtcaat cagccatgag ttctcatcac gggcgacaat ctgctcttcc agcccctgac
    13261 cgaccagatc gcccatcagc gtggtcatca cgtcgttgag ggtgatgatc cccaccacca
    13321 gcgcgtactc gttcatgatc accgcgaagt cttcacctgc ggttttaaaa ctttccaacg
    13381 cctctgaaag ggttaacgta tccggcacaa tcagcgtgtt gcgaatttgt acgccgctgt
    13441 tcagtgccag gctttggtta gccagcacgc ggttcagcag gtctttagaa tcgacataac
    13501 cgatgatgtg gtcaatatct tcattacaga cgaggaactt agagtgcgga tgttccgcca
    13561 ccttattctt caggctttgc tcatcttcgt ggagatcaaa ccaaatcacg ttttcacgcg
    13621 gtgtcattga agacggaacg gtacgggatt ccagctcaaa gacgttttca atcagctcgt
    13681 gttcctgttt acgtaacacg cccgccagcg caccggcttc cactaccgcg tagatgtcat
    13741 cagaagtgat gtcatcttta cgtaccattg gcagtttgaa aatacggaag atcatgttcg
    13801 ccaggccgtt gaagaaccac accagcgggg tgcaaacgta caggcagaag cgcatcgggt
    13861 tgatgatacg caaagccacc gcttctggcg caatcatacc gatgcgtttc ggggttaaat
    13921 ccgcaaacag gataaacatg ccagtcacta acgagaaaga gagaataaag ctcagttgct
    13981 cagagagttc tgccgacata tagcgggaga acaggctgtg aaaagctgga gaaaatgccg
    14041 catcaccgac gataccgccg agaatcgcaa ctgcgttcag accgatttgg accaccgtaa
    14101 agaacatgcc gggattttcc tgcatattca gaacgcgttg ggcatttata ttgccttcat
    14161 cagccagcag tttaagtttg attttgcgtg aggcggcaag tgagatctca gacatcgaga
    14221 agaacgcact tacagcgatc aagcagagta tgactaaaat actgtttaac atatcttatc
    14281 cgacctttca ggccggatcc tcggaagggg aagttgatta atttgtgtga aatacacatt
    14341 gaaagccgat tcgaagaaag tgaaccggca ttttcagtgg ctagtatagc gtaaggtact
    14401 gtaaagtcgc cagagggtta aaattcagac tgctgataaa tgcaatactg cctgatgcgc
    14461 tatgccgatc aggcctacgc agtccctgca atatattgaa tttggacgat tttgtaggcc
    14521 tggtaaggca ttcacgccgc attcggcata aacaaaacgc actttatcaa caatctacat
    14581 ttgtacccgt aacgctatgc ttccggtggc agacagacgc caattccgcc aattccacag
    14641 taaccatacg ggtttttatg cagatattgc tggtggtcat cttcggcata ataaaacggt
    14701 gtggcgttag cgatttccgt ggtgatgtga cgatcgtcat cggcggcaag catcgccgcc
    14761 tgaaaacgtt ccagactggc gcgagctgcg gcatcctgtt ctggggtcag cggataaatc
    14821 gctgaacgat actgcgtgcc gtggtcattg ccctgacgca ttccctgggc gggatcgtga
    14881 ttctcccaaa atacctgtag caactgctca tagctgatga cagaaggatc gtaaacaatg
    14941 cgtaccgctt cggcatgacc cgtatcaccg gagcacactt cccgataagt cggatttggc
    15001 gtatagccgc cggtatagcc tgcggcggtg ctgtaaacgc cgggtaactg ccagaacaga
    15061 cgctccacac cccagaaaca acccatcgca aaaatggcaa tctccattcc gtcaggtaca
    15121 ttggtcattg agtgaccgtt gaccgcatgc agcgtggcta cgggcatcgg ggtgttacgt
    15181 ccaggcaggg catcggcggg ggaaaccaga tgctttttat caaataaact catggtgtcg
    15241 ctctcccgaa aatcggtcat tggggttaag gttgtaacaa gaggcgtatt tgcacacaat
    15301 aaccaccgtg aataggtcta aagtaaaaca taagaaatat ttggggttta gtctgctttt
    15361 taatccatat tactggattt ttgttaagcc gtttaacggc gttccagggg caggaaaaaa
    15421 ggatattcag gagaaaatgt gcgctatatc cgacagttat gctgtgtaag cttactctgc
    15481 ttaagcggat ctgccgtcgc cgcgaacgtc cgtctacagg tcgaggggtt atcgggacag
    15541 ctggaaaaga acgttcgtgc gcagctttct acgattgaaa gtgatgaagt gacgccagac
    15601 cgtcgctttc gcgcacgcgt cgatgatgcc atccgcgaag gtctgaaagc gctgggttat
    15661 taccagccga ccattgaatt tgatctccgt ccaccgccaa agaaagggcg gcaggtattg
    15721 atcgccaaag tcacgccagg cgtgccggtg ttaattggcg gcaccgatgt ggtattgcgc
    15781 ggcggcgcgc ggaccgataa agactatttg aaattgctcg atactcgccc ggctattggc
    15841 acggtactga accagggcga ttatgaaaat ttcaaaaagt ccttaaccag cattgcgttg
    15901 cgtaaaggtt atttcgatag cgaatttacc aaagcgcagc tgggcattgc gctcggcctg
    15961 cataaagcct tctgggatat tgattataac agtggcgaac gttaccgctt tgggcatgtg
    16021 acctttgaag gatcacaaat ccgcgatgaa tacctgcaaa atctggtgcc gtttaaagag
    16081 ggcgatgagt acgaatcgaa agatctggcg gaacttaacc gtcgactttc tgctaccggc
    16141 tggtttaact cggtcgtagt ggctccacaa tttgataaag cgcgcgaaac gaaagtatta
    16201 cccttgacgg gcgtggtttc gccgcgaact gaaaacacca tcgaaaccgg ggtcggttac
    16261 tctacggacg tgggaccgcg cgtgaaagct acgtggaaaa agccgtggat gaactcttat
    16321 ggtcacagtc tgaccaccag taccagtatt tccgcgccgg aacagaccct cgacttcagc
    16381 tataaaatgc cgctgctgaa gaatccactg gaacaatatt atttggtgca gggcggtttt
    16441 aagcgcactg acctgaacga taccgaatct gactccacta cgctggtggc ttctcgctac
    16501 tgggatctct ccagcggctg gcagcgtgcc attaacctgc gctggagtct cgaccacttt
    16561 actcagggtg aaattaccaa caccacgatg ctgttttatc ctggggtgat gattagccgc
    16621 acgcgttctc gtggtggcct gatgccaacc tggggcgact cgcaacgcta ctctatcgac
    16681 tactccaaca cggcctgggg ctcagatgtc gatttctccg ttttccaggc gcagaacgtc
    16741 tggatccgca cactgtacga tcgccatcgt ttcgttacac gcggcacgct gggctggatt
    16801 gaaaccggtg atttcgacaa agtaccgccg gatctgcgtt ttttcgccgg gggcgatcgc
    16861 agtattcgcg gctacaaata caaatctatc gccccgaaat acgccaacgg tgacctgaaa
    16921 ggggcctcga agttgataac cggatcgctg gaataccagt acaacgtgac cggaaaatgg
    16981 tggggcgcgg tgtttgtcga tagtggcgaa gcggtaagcg atattcgccg cagcgacttt
    17041 aaaaccggta ccggggtcgg cgtgcgctgg gaatcgccgg tcgggccaat caaactcgat
    17101 tttgccgtac cggtcgcgga taaagacgaa cacgggttac agttttacat cggtctgggg
    17161 ccagaattat gagtttatgg aaaaaaatca gcctcggcgt ggttatcgtt atcttactgt
    17221 tgctgggatc ggtggcgttt ctggtgggca ccaccagcgg cctgcatctt gtatttaaag
    17281 cggcggatcg ctgggtgcca ggactggata ttggcaaggt caccggcggc tggcgcgatc
    17341 tcaccttgtc tgacgttcgt tatgagcagc caggcgtggc ggtaaaagcg ggtaatctgc
    17401 atctggcggt cggtcttgag tgcctgtgga acagtagcgt ttgtattaat gacctggcgc
    17461 tgaaagacat tcaggtcaac atcgacagta aaaaaatgcc tccttctgaa caggttgaag
    17521 aagaggaaga tagcggtccg ctggatctct ccacgccgta tcccatcacc ctgacacggg
    17581 tggcgctgga caacgtcaac atcaagattg atgacaccac ggtgtcggtg atggacttca
    17641 cctccggcct gaactggcag gagaaaaccc tgaccctgaa accgacgtcg ctgaaaggcc
    17701 tgctgattgc tctgccgaaa gtggcggaag tggcgcagga agaagtggtc gaaccgaaaa
    17761 ttgaaaatcc gcagccggag gaaaagccgc tcggcgaaac gctgaaagat ctcttttctc
    17821 gcccggtatt gccggaaatg accgacttgc atttgccgct taacctgaac attgaagagt
    17881 ttaaaggcga gcagttacgc gtgacgggcg acacggacat caccgtgcgc accatgctgc
    17941 tgaaagtgag cagcattgac ggcaatacta aactggacgc cctggatatc gattccagtc
    18001 aagggatcgt caacgccagc ggcacggcgc agctgtcaga caactggccg gtggatatca
    18061 ccctcaacag cacactgaac gtggagccgt tgaaaggtga aaaagtgaag ctaaaagtgg
    18121 gcggcgcgct gcgcgaacag ctggagattg gcgttaatct ttccggtccg gtggatatgg
    18181 atttacgcgc ccagacgcga ctggcggaag ccggattgcc actcaacgtg gaagtgaaca
    18241 gcaaacagct ttactggccg ttcaccggtg agaagcagta tcaggcggat gatctgaaac
    18301 tgaaacttac cggcaaaatg accgattaca cgctctctat gcgtacggca gtgaagggac
    18361 tggagatccc gccagccacc attacccttg atgccaaagg taatgaacag caggtcaatc
    18421 tcgacaaact caccgtcgcg gcgctggaag ggaaaactga actcaaggcg ttgctcgact
    18481 ggcagcaggc cattagttgg cgcggtgagc taacgcttaa cggcattaac accgccaaag
    18541 agatcccaga gtggccgtcg aaactcaatg gcttgattaa aacccgcggt agcctgtacg
    18601 gcggcacctg gcagatggag gtgccagaac tgaagctgac cggtaacgtc aaacagaaca
    18661 aagtgaacgt tgacggcacg ctgaaaggca acagttatat gcagtggatg atcccagggc
    18721 ttcatctgga actggggcca aacagtgccg aagtgaaagg cgagctgggg gtaaaagatc
    18781 tcaatcttga tgccaccatc aacgcgccgg ggctggataa cgcgctgccg gggcttggcg
    18841 gtacagcgaa agggctggtg aaagttcgcg gcacggtgga agcgccacaa ctactggcgg
    18901 acatcaccgc gcgcggcctg cgctggcagg agctaaccgt ggcgcaggtt cgcgtggaag
    18961 gcgatatcaa atccaccgtt cagattgcgg gtaaactcga cgtgcgcgtt gagcaaattt
    19021 cacagccgga tgtaaatatc aacctcgtca ccctgaatgc caaaggcagc gaaaaacagc
    19081 acgagttaca gttgcggatt cagggcgagc cggtttccgg gcagcttaat ctggcaggaa
    19141 gttttgatcg caaagaagaa cgctggaagg gaactcttag caatacccgc ttccagacgc
    19201 cggtcggccc gtggtcgctg acccgcgata ttgcgctgga ttaccgcaat aaggagcaaa
    19261 aaatcagcat cgggccacac tgctggctta acccgaatgc ggaactgtgc gtgccgcaaa
    19321 ctatcgatgc gggggccgaa gggcgtgcgg tggtgaatct caaccgcttc gacctcgcca
    19381 tgctgaaacc gtttatgcca gaaaccactc aggccagcgg tatcttcacg ggtaaagcgg
    19441 atgttgcctg ggacaccacg aaagaggggc tgccgcaggg cagtatcacc ctttcggggc
    19501 gtaacgtgca ggtaacgcaa accgtcaacg atgcggcgct gcccgtggcg tttcagacac
    19561 tgaatctgac ggcggaattg cgtaacaacc gtgccgaatt gggctggacc atccgcctga
    19621 ccaataacgg ccagtttgat ggacaggtgc aggtgaccga tccgcaaggc cgccgtaatc
    19681 ttggtggcaa cgtcaatatc cgtaacttca accttgcgat gataaacccc atctttaacc
    19741 gtggggaaaa agcagcgggg atggtgagtg ccaacttgcg tctgggtggt gatgtgcaaa
    19801 gcccgcagtt gtttggtcag cttcaggtta cgggtgtgga tatcgacggc aactttatgc
    19861 cgtttgatat gcaaccgagc cagcttgcgg tcaactttaa tggtatgcgc tcgacgcttg
    19921 ccggtacagt acggacccag cagggtgaaa tctacctgaa cggtgatgcc gactggagcc
    19981 aaattgaaaa ctggcgggcg cgggtaacgg cgaaaggcag taaagtgcgg atcaccgtgc
    20041 cgccgatggt acgaatggat gtatcgccag atgttgtctt cgaggctaca ccaaacctgt
    20101 ttaccctcga tggtcgcgtg gatgtcccgt gggcgcgcat cgtggtgcac gatctgccgg
    20161 aaagcgcagt aggcgtctcc agcgatgtgg tgatgcttaa cgataacctg caacctgaag
    20221 agccgaaaac ggcgtcgatt ccgattaaca gtaacctgat tgtccacgtt ggcaacaatg
    20281 tgcgcattga cgcctttggc ctgaaagcgc ggctgacggg cgatcttaac gtcgttcagg
    20341 acaaacaagg gctgggcctg aacgggcaga tcaacatccc tgaagggcgc ttccatgcct
    20401 atggtcagga tctgattgtg cgtaaaggtg agttactgtt ctctggtccg ccggatcaac
    20461 cgtatcttaa tattgaagct attcgtaacc cggatgctac agaagacgac gtaatcgccg
    20521 gagttcgcgt cactggtctg gcggacgaac cgaaagcgga gatcttctct gacccggcga
    20581 tgtcgcaaca agctgcattg tcttatttgc tacgtggaca agggctggag agcgatcaga
    20641 gcgacagtgc ggcaatgacc tcgatgctga ttggtctggg ggttgcgcaa agtggccaga
    20701 ttgtgggtaa aatcggcgag acgtttggcg taagcaattt agcgctcgac acccagggag
    20761 taggcgactc ctcccaggta gtggtcagcg gctatgtatt gccaggtctg caagtgaaat
    20821 atggcgtggg tatatttgac tctatagcaa cactcacgtt acgttatcgc ctgatgccta
    20881 agctatatct ggaagccgtg tctggtgtag accaggcact ggatttgctc tatcagttcg
    20941 agttttagca atgcgaatat ttgtctacgg cagtttacgc cacaaacaag gcaacagtca
    21001 ctggatgacc aatgcccagt tactgggcga tttcagtatc gataactacc agttgtatag
    21061 cctgggccac tatccaggcg cagttccggg gaacggaaca gtacacggtg aagtttatcg
    21121 tattgacaac gccacgctgg ccgaacttga tgccttgcgc accaggggcg gtgaatacgc
    21181 gcgccagttg attcagacgc cgtacgggag tgcatggatg tacgtttatc aacgacccgt
    21241 cgatggatta aagctaattg aaagcggcga ctggttagac agggataagt aaccatatgc
    21301 atacgccacc ttcgggtggc gttgtttttt gcgagacgac tcgcattctg ttttgtaatt
    21361 ccctcaccct ttgcttttct ctccgagccg ctttccatat ctgttaacgc ataaaaaact
    21421 ctgctggcgt tcacaaatgc gcaggggtaa aacgtttcct gtagcaccgt gagttatact
    21481 ttgtataact taaggaggtg cagatgcgta ttaccataaa aagatggggg aacagtgcag
    21541 gtatggtcat tcccaatatc gtaatgaaag aacttaactt acagccgggg cagagcgtgg
    21601 aagcgcaggt gagcaacaac caactgattc tgacacccat ctccaggcgc tactcgcttg
    21661 atgaactgct ggcacagtgt gacatgaacg ccgcggaact tagcgagcag gatgtctggg
    21721 gtaaatccac ccctgcgggt gacgaaatat ggtaaagaaa agtgaatttg aacggggaga
    21781 cattgtgctg gttggctttg atccagcaag cggccatgaa cagcaaggtg ctggtcgacc
    21841 tgcgcttgtg ctctccgttc aagcctttaa tcaactggga atgacgctgg tggcccccat
    21901 tacgcagggc ggaaattttg cccgttatgc cggatttagc gttcctttac attgcgaaga
    21961 aggcgatgtg cacggcgtgg tgctggtgaa tcaggtgcgg atgatgtatc tacgcgcccg
    22021 gctggcaaag cgtattggtc tggctgcgga tgaggtggtg gaagaggcgt tattacgctt
    22081 gcaggcggtg gtggaataag gtgtgtttat ttatcgcggg cataaaaaaa cccttactaa
    22141 ccgaagctcg gcgttcaggg ttattgcgct agaagaactt atttattctt tgcgcgctcg
    22201 aaggaagcaa cgatttcagc tttagcggct tctgcgtttt cccaaccttc aactttcacc
    22261 cacttgcctt tttcgaggtc tttgtagtgc tcgaagaagt gagcgatctg cgctttcagc
    22321 agctctggca gatcgttaac gtctttaatg tgatcgtatt ctttgctcag cttgctgtgc
    22381 ggaaccgcaa ccagtttcgc atcttcaccg gcttcgtcgg tcattttcag aacgccaacc
    22441 ggacggcaac ggatcacaga acccggctgc agcgggtacg gagtcgggac cagtacgtca
    22501 accgggtcac cgtccagaga cagggtgtgg ttgatgtaac cgtagttgca cggatagaac
    22561 atcgcggtgg acatgaagcg gtcaacgaac agtgcgccgc tctctttgtc gatttcgtat
    22621 ttgatcggat ctgcgttagc cgggatctca ataacaacgt agatgtcttc cggcagatct
    22681 ttacccgcag ggacgttgag taagctcatg tctgtttcct ttaaaaatat gtggcaaaca
    22741 agtgccgagt attatagcca actcgcgccg aatgtctttg cttgttttcg tcttcatttt
    22801 cctttttcgc cagttttaag acggtataca gagcaggaaa atccataacc gtagccgcat
    22861 ttttcatagt gagatgaaag cgattacaaa cttgtgatta acgttttatt tacttttttg
    22921 aagtgtgatg taacgcaatc cgttacataa cgaattgtct atagtttttt cgcgaacatc
    22981 ttttcaccaa taataattac cccgacgagg acaaccctat gtggaaacgc ttacttgtag
    23041 tctctgcagt ctcggcagcc atgtcgtcta tggcgttggc cgctccatta accgtaggat
    23101 tttcgcaggt cggatcggaa tccggctggc gcgctgcaga aaccaatgtg gcgaaaagtg
    23161 aggccgaaaa acgcggaatt acgttgaaaa ttgccgatgg tcagcaaaag caggaaaacc
    23221 agattaaagc ggtacgttcc ttcgttgcac aaggggtgga tgcgatcttt atcgctccag
    23281 tggttgcgac gggttgggaa ccggtattaa aagaggcgaa agatgccgaa atcccggtct
    23341 tcttgcttga ccgttccatc gatgtgaaag acaaatctct ctatatgacc accgtcactg
    23401 ccgacaacat cctcgaaggc aagttgattg gtgactggct ggtaaaagaa gtgaatggca
    23461 aaccatgcaa cgtggtggaa ctgcagggca ccgttggggc cagcgtcgcc attgaccgta
    23521 agaaaggctt tgccgaagcc attaagaatg cgccaaatat caaaatcatc cgctcgcagt
    23581 caggtgactt cacccgcagt aaaggcaaag aagttatgga gagctttatc aaagcggaaa
    23641 acaacggcaa aaacatctgc atggtttacg cccataacga tgacatggtg attggtgcaa
    23701 ttcaggcaat taaagaagcg ggcctgaaac cgggcaaaga tatcctcacg ggttccattg
    23761 acggcgtacc ggatatctat aaagcgatga ttgatggcga agcgaacgcc agcgttgaac
    23821 tgacgccgaa catggcaggc cccgcttttg acgcgctgga gaaatacaaa aaagacggca
    23881 ccatgcctga aaagctgacc ctgaccaaat ccacccttta tctgcctgat accgcaaaag
    23941 aagagttaga gaagaagaaa aatatggggt attgagggtt gctatgcctg atgccgattc
    24001 gtaggccgga taaggcgctc gcgccgcatc cggcgatggt gcactgaagc ctgatgcgac
    24061 gcttaccgcg tcttatcatg cctactggga gcacgcttta caccggggga aaccatgacg
    24121 accgaccaac accaggagat cctccgcacc gaaggattaa gtaaattttt ccccggcgtc
    24181 aaagcgttag acaacgttga tttcagcctg cgccgtggcg aaatcatggc gctgcttggt
    24241 gaaaacgggg cgggaaaatc aacgctaatc aaagcattaa ctggtgtata ccacgccgat
    24301 cgcggcacca tctggctgga aggccaggct atctcaccga aaaataccgc ccatgcacaa
    24361 caactcggta tcggcaccgt ctatcaggaa gtcaacctgc tacccaatat gtcggttgct
    24421 gataatctat ttataggccg cgaacccaaa cgtttcggcc ttctacgccg taaagagatg
    24481 gaaaagcgcg ccaccgaact gatggcatct tacggtttct ccctcgacgt gcgcgaaccg
    24541 cttaaccgtt tttcggtggc gatgcagcaa atcgtcgcca tttgccgggc tatcgatctc
    24601 tccgccaaag tgctgatcct cgatgaaccc accgccagcc tcgacaccca ggaagtggag
    24661 ctattatttg gcctgatgcg tcagttgcgc gatcgcggcg tcagcctgat ctttgtcact
    24721 cactttctcg atcaggtcta tcaggtcagc gatcggatca ccgtcttacg caacggcagt
    24781 ttcgtaggct gtcgggaaac gcgcgagcta ccgcagatcg aactggtaaa aatgatgctg
    24841 gggcgcgagc tggataccca cgcgctacag cgtgccgggc gaacattgtt gagcgacaaa
    24901 cccgttgccg cgttcaaaaa ttacggcaaa aaaggaacga tagcaccgtt tgatctcgaa
    24961 gtgcgccccg gcgagatcgt cggtctggct ggcttgctgg gatccggacg taccgaaacc
    25021 gccgaagtga tcttcggtat caaacctgct gacagcggca cggcgttgat caaaggcaaa
    25081 ccgcaaaccc tgcgatcgcc acatcaggct tcagtactgg gcatcggatt ctgcccggaa
    25141 gacaggaaaa ccgatggcat catcgctgcc gcctcggtgc gggaaaatat catcctcgct
    25201 ctccaggccc agcgcggctg gctacgaccc atttcccgca aagagcagca agagattgcc
    25261 gaacgcttta tccgccagct tggcattcgc acaccttcaa ctgaacaacc gattgaattt
    25321 ctctctggcg gcaatcagca aaaagtgttg ctttcacgtt ggctactgac acgaccgcaa
    25381 tttctgatcc tcgacgagcc aacccgcggc attgatgttg gtgcccacgc cgagatcatc
    25441 cgcctgattg aaacgctgtg tgctgacggt ctggcgctgt tggtgatctc ctccgaactg
    25501 gaagagctgg tgggctatgc cgatcgggtg attatcatgc gcgatcgcaa acaggtggcg
    25561 gagatcccgc tggcagagct ttccgttccg gcgatcatga acgccattgc ggcgtaagga
    25621 gaacagtgtg atgcctcaat ctctcccgga cactacgccg ccgaaaaggc gctttcgctg
    25681 gccaacggga atgccgcagc tggcggcgct attgctggtg ctgctggtcg atagcctggt
    25741 agccccgcat ttctggcagg tggtgcttca ggatgggcgt ttgttcggta gccctatcga
    25801 cattcttaac cgtgcagctc ccgttgcgtt actggcgatt ggtatgacgc tggtgatcgc
    25861 caccggtggg attgatctct ccgtaggggc ggtgatggct atcgccggag ccacaacggc
    25921 tgcgatgacg gtcgcgggat tcagcctgcc gattgttttg ttaagcgccc tgggcaccgg
    25981 catcctggcg ggattgtgga acggcatact ggtagcgatc ctcaaaattc agccgtttgt
    26041 cgccaccctg atcctgatgg tcgccgggcg cggcgtggcg caactgatca ccgccgggca
    26101 gatcgtcacg tttaactcgc cggatctctc atggtttggc agcggatcgc tgttgttcct
    26161 gccaacgccg gtcattatcg cggtgctgac gcttctcctg ttctggctgt tgacccgcaa
    26221 aacggcgctg gggatgttta tcgaagccgt tggtatcaac attcgggcgg caaaaaatgc
    26281 cggggtaaac acgcggatca tcgtcatgct cacctacgtg ttgagcgggc tgtgtgcggc
    26341 gattgcgggc attatcgtgg cggcggatat tcgcggtgcc gatgccaata acgccgggtt
    26401 atggctggag ctggacgcca ttcttgcggt ggtgattggc ggcggatcgc tgatgggcgg
    26461 gcgctttaac ctgctgctct cagtcgtggg ggcgctgatt attcagggga tgaacaccgg
    26521 aattttactt tctggctttc cgccggagat gaaccaggtc gtaaaagcgg tggtggtgct
    26581 ttgtgtgctg attgttcagt cgcaacgctt tatcagtctg attaaaggag tacgtagcca
    26641 tgataaaacg taacttgccg ctgatgatca ccatcggcgt ctttgtgctg ggctatctct
    26701 actgcctgac ccagtttccc ggttttgctt ccacaagagt gatctgcaat atcctgaccg
    26761 ataacgcctt tcttgggata atcgccgttg gcatgacctt tgtgatcctc tctggcggta
    26821 tcgatctctc cgtcggttcg gtgatcgcct ttactggcgt gtttctggca aaagtgattg
    26881 gcgatttcgg cctctcgccg ctgctggcgt ttccgctagt gctggtgatg ggctgtgcct
    26941 ttggcgcatt tatggggctt ttgatcgacg ccctgaagat cccggcattt atcattaccc
    27001 tggcaggaat gttctttttg cgcggcgtca gctatctcgt ttcagaagag tcgatcccga
    27061 taaaccatcc catttatgac acgctctcaa gccttgcgtg gaaaatccct ggcggcggtc
    27121 gcttaagtgc gatgggactg ctgatgctgg cggtggtggt tatcggcata ttcctcgcgc
    27181 atcgtacccg ttttggtaat caggtatacg ccattggcgg caacgcaacg tcggcaaacc
    27241 tgatggggat ttctacccgc agcaccacta ttcgcattta tatgctctcc accggactgg
    27301 caacgctggc ggggattgtc ttctcgattt atacccaggc cggatatgcg ctggcgggcg
    27361 taggtgtgga actggatgct atcgcctcag tggtaattgg cggtacgctt ttgagcggtg
    27421 gcgttggaac ggtattaggg acgctttttg gcgtggcgat tcaggggcta attcagactt
    27481 atatcaactt tgacggtacg ctgagttcct ggtggacgaa aatcgccatc ggcattttgc
    27541 tgttcatttt tattgcctta cagcgtggat taacggtgct gtgggagaat cgtcagagtt
    27601 cgccagtgac aagagtcaat attgcgcagc gataaaaacg cctctccgtg tggagaggcg
    27661 caggagatta cgcgtccggg aattcacgga taaagcgttc gacatcttca accatatggt
    27721 cgttgccgac aaagaacgaa cggcgctggt gcagagtttc cgggatgata tccagaatac
    27781 gctctttgcc atcgctcgct ttaccgcccg cttgttccgc caggaatgcc atcgggttgc
    27841 actcatacag caaacgcagt ttgccgtccg ggtggctggc ggtgcttggg tagagataaa
    27901 taccgccttt cagcaggtta cggtggaaat ccgcgaccag tgaaccgata taacgtgagg
    27961 tatacgggcg gttggtggat ttatcttctt cctggcagaa tttaatgtac ttcttcaccc
    28021 cgttcggaaa cttaatgtag tttccttcgt tgatggagta ggttttgcct ttctccggga
    28081 agcgcatccg ttcctggcac aggcagaaaa cgcccagcga aggatcgtaa gtaaaggcgt
    28141 gaacaccgca tccggtggtg taaaccagca tggtagagga gccgtatacc acgtaacctg
    28201 ccgcaacctg tttgttacca ggctggagga aatcttcttc cgttaccggc gtgccaacag
    28261 gcgtaacgcg gcggtagatg gagaaaatgg taccgacaga gacgttaaca tcgatgttgg
    28321 acgagccatc cagggggtcc atcagcacca cgtattttgc gtgttcacag ccttcaaaga
    28381 cgacaatctc atcttcttct tcagaggcaa tgcccgcaac gatatcgcgt gctttcagtg
    28441 cggctttcag tttttcatta gcgaacaagt cgagtttctg ctgaacctcg ccctgcacgt
    28501 tctcagcacc gctggcaccc aggatatcaa ccagtcctgc tttgttgata tcgcgatgga
    28561 taatcttggc gcccagtttt attgccgaca gcaaagcagt gagctcaccg gtagcatgag
    28621 aaaactcgtg ctgcttttcg acaataaatt cacctaacgt tttcataaaa ctttccctgc
    28681 aatgtttatg gagtaaagcg accgcaacaa tcttaacaaa taatctcaat gttgcgctca
    28741 ggtgaatcgc gccagcaaat tgcggattat cctgaaatgc gtttctcact tgcccgacat
    28801 atgcgtaaaa tgagcggcag attaaaaaag gatagtgacg tatgcgcatt catattttag
    28861 gaatttgtgg cacatttatg ggcggtctgg cgatgctggc gcgccagtta ggccatgaag
    28921 taacgggttc ggacgccaat gtgtatccgc cgatgagcac cttacttgag aagcaaggca
    28981 ttgagctgat tcagggttac gatgccagcc agctcgatcc gcagccggat ctggtgatta
    29041 ttggcaacgc catgacccgt ggaaatccgt gtgtggaagc ggtactggaa aaaaacatcc
    29101 cttatatgtc aggtccacag tggctgcacg attttgtgct gcgcgaccgc tgggtgctgg
    29161 ccgttgccgg tacacacggc aaaactacca ccgcgggaat ggcgacctgg attctggaac
    29221 agtgcggtta caaaccggga tttgtgatcg gcggtgtgcc ggggaacttt gaggtttcgg
    29281 cgcgtctggg cgaaagcgac ttctttgtta tcgaagcgga tgagtatgac tgcgccttct
    29341 tcgacaaacg ctctaaattt gtccattact gcccgcgtac gctgatcctc aacaaccttg
    29401 agttcgatca cgccgatatc tttgacgacc tgaaagcgat ccagaaacag ttccaccatc
    29461 tggtgcgtat cgttccgggg cagggccgta ttatctggcc ggaaaatgac atcaacctga
    29521 aacagaccat ggcgatgggc tgctggagcg agcaggagct ggtgggtgag cagggtcact
    29581 ggcaggcgaa aaagctgacc actgacgctt ctgaatggga agtcttgctg gatggcgaaa
    29641 aagtgggcga agtgaaatgg tcgctggtgg gcgaacataa tatgcacaat ggcctgatgg
    29701 cgattgcggc ggctcgccat gttggtgtag cgcctgcaga tgccgctaac gcgctgggtt
    29761 cgtttattaa cgctcgtcgc cgtctggagt tgcgtggtga agcgaatggc gtgacggtat
    29821 atgacgattt tgcccatcac ccgacggcaa ttctggcaac gcttgcggcg ctgcgtggca
    29881 aagttggtgg tacggcgcgc attattgctg tgctggagcc gcgctcgaat accatgaaaa
    29941 tggggatctg caaagacgat ctggcacctt cattaggtcg tgccgatgaa gtcttcctgc
    30001 tgcaaccggc gcatattccg tggcaggtgg cagaagtggc agaagcctgc gttcagcctg
    30061 cacactggag tggcgatgtg gatacgctgg cagatatggt ggtgaaaacc gctcagcctg
    30121 gcgaccatat tctggtgatg agcaacggcg gttttggtgg gatccatcag aaactgctgg
    30181 atggtctggc gaagaaggcg gaagctgcgc agtaattcgg cctcagcctg atatagtgtg
    30241 cgtaggccgg ataaggcgtt cacgccgcat ccggcatttg agcatagtgc ctgatgcgac
    30301 gcttgatgcg tcttatcagg cctacaggtg caccgcatcc ggcaaaccat actcacatca
    30361 acaacgaaaa ttacccttca ttctccgcca gttcgcgcag atactggaaa atctggcgtg
    30421 cggatttcgg cggcttattc ccttctttct ctttcttcgc gttgcggatc agagtacgca
    30481 gctgctgacg atcggcatcg tgccacagat ttaatacctc agcgatggcg tcatcacctt
    30541 gatcgatcag acgatcgcgc aggttttcca gtttatggaa cagcaccacc tgctggttgt
    30601 gacggttttt cagcttatcc agcgcctgac ggatcggttc tacatcgcgc tggcgcagca
    30661 tcttaccgat gagctgcaac tggcggcggc gaccttccat cttaatacgc tgggcgagct
    30721 caatagccgc gcgcagatcc gcatctaacg ggatcttatc cagcgcgttt ttccccagat
    30781 caacaatttc cgcgccaagg cgttttagct cctcggcatc acgtttaatt tcacttttac
    30841 tgacccagat aatttcatcg tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc
    30901 agtcttcggg ctgcttagtc atctcaggct ccttaaaaaa agaggctaat gttaccagtt
    30961 aagatgcgca ctgaaaaacg gttctctgtt agacttcaga gaaactctct acattatggc
    31021 acttgcaatg aaagtaatct ctcaagttga agcgcagcgc aagattctgg aagaagcagt
    31081 ttccactgcg ctggagttgg cctcaggcaa atcggacggt gcggaagttg ccgtcagcaa
    31141 gaccaccggc attagcgtaa gcacgcgtta tggtgaagtg gagaatgttg aattcaatag
    31201 cgatggcgcg ctggggatca ctgtttatca ccagaaccgc aaaggtagcg catcatccac
    31261 cgatttaagc ccgcaggcca ttgcccgtac tgtacaggcg gcgctggata ttgcccgtta
    31321 tacctcgcca gatccctgtg ccggcgtggc agacaaagag ctgctggcct ttgacgcacc
    31381 agatctcgac ttgttccacc ctgcggaagt ttccccggat gaagccattg aactggcggc
    31441 ccgcgcagaa caggcggcat tgcaggcgga caaacgcatt accaataccg aaggtggcag
    31501 ctttaacagc cactacggtg tcaaagtttt tggcaacagc cacggcatgt tgcagggtta
    31561 ctgctcaacg cgtcattcgc tctccagctg tgtaattgcc gaagaaaatg gcgatatgga
    31621 gcgtgattac gcctacacca ttggccgcgc gatgagcgat ctgcaaacgc cagagtgggt
    31681 cggggccgac tgtgctcgcc gtactttatc gcgcctgtca ccgcgtaaac tctccaccat
    31741 gaaagcgcca gtcatttttg ccaatgaagt ggcaaccggg ctttttggcc atctggtggg
    31801 ggcgatagcg ggtggctcgg tttaccgtaa atctaccttt ctgctggatt cgctgggtaa
    31861 acaaattctg ccggactggc tgaccattga agagcatccg catctgctga aagggctggc
    31921 gtcgacgcca ttcgacagcg aaggtgtgcg caccgagcgt cgcgatatta ttaaagatgg
    31981 catcctgact cagtggctgc tgaccagcta ctcggcgcgg aaactggggc tgaaaagcac
    32041 cggacatgcg ggcggtattc acaactggcg gattgccgga caagggctaa gcttcgaaca
    32101 aatgctcaaa gagatgggca ccgggctggt ggtgacggag ctgatgggcc agggcgtgag
    32161 tgccattacc ggtgattatt cccgtggtgc agcgggcttc tgggtagaga atggcgaaat
    32221 tcagtatccc gttagtgaaa tcaccatcgc aggtaattta aaagatatgt ggcgcaatat
    32281 tgtcaccgtc ggtaacgata ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt
    32341 gccggagatg aaaatcgccg ggcagtaaaa aactggcgcg acctgccgcg ctataaacta
    32401 aaaaattaca caaaatcatt cgcactgcat cgagacggaa agtgaatgag ggccggggag
    32461 cgtactcgca gtacgtgacc cgggcgaatg agcgtagccg tcgatgaggc agtgtgaagg
    32521 atgaagtgta aataaaaaag gaagtgagca atgcgtaaaa gcctgttagc tattcttgca
    32581 gtctcctcgt tggtattcag ttctgcgtcg tttgccgctg atcttgaaga
//
LOCUS       DAZGOB010000046        32255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGOB010000046 DAZGOB010000000
VERSION     DAZGOB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.046"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            12..884
                     /locus_tag="ACHLIF_002296"
     CDS             12..884
                     /locus_tag="ACHLIF_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9606302.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            1253..4099
                     /locus_tag="ACHLIF_002297"
     CDS             1253..4099
                     /locus_tag="ACHLIF_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFO9606303.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLTNHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATVTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQNVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTQDAVLSRAVAKGDSPVTFHK
                     LTTTNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            4427..5245
                     /locus_tag="ACHLIF_002298"
     CDS             4427..5245
                     /locus_tag="ACHLIF_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9606304.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLESLQREGFQPFFAYQIRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPSRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRTIHSIDTDIKLNRALWVMAETLLESMR"
     gene            5337..5822
                     /locus_tag="ACHLIF_002299"
     CDS             5337..5822
                     /locus_tag="ACHLIF_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9606305.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRMENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            5838..6314
                     /locus_tag="ACHLIF_002300"
     CDS             5838..6314
                     /locus_tag="ACHLIF_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO9606306.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            6377..6598
                     /locus_tag="ACHLIF_002301"
     CDS             6377..6598
                     /locus_tag="ACHLIF_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9606307.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            6672..7079
                     /locus_tag="ACHLIF_002302"
     CDS             6672..7079
                     /locus_tag="ACHLIF_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9606308.1"
                     /translation="MSDTLHGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCEAD
                     TLGSCSFVYMAVYATRFSNQLPKASVHQLTHYF"
     gene            complement(7620..8867)
                     /locus_tag="ACHLIF_002303"
     CDS             complement(7620..8867)
                     /locus_tag="ACHLIF_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO9606309.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(8939..9853)
                     /locus_tag="ACHLIF_002304"
     CDS             complement(8939..9853)
                     /locus_tag="ACHLIF_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO9606310.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            10069..11502
                     /gene="cscA"
                     /locus_tag="ACHLIF_002305"
     CDS             10069..11502
                     /gene="cscA"
                     /locus_tag="ACHLIF_002305"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO9606311.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(11510..12505)
                     /locus_tag="ACHLIF_002306"
     CDS             complement(11510..12505)
                     /locus_tag="ACHLIF_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9606312.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            12748..12966
                     /locus_tag="ACHLIF_002307"
                     /pseudo
     CDS             12748..12966
                     /locus_tag="ACHLIF_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            12984..14312
                     /gene="dsdA"
                     /locus_tag="ACHLIF_002308"
     CDS             12984..14312
                     /gene="dsdA"
                     /locus_tag="ACHLIF_002308"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO9606313.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKMTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(14420..15958)
                     /gene="emrY"
                     /locus_tag="ACHLIF_002309"
     CDS             complement(14420..15958)
                     /gene="emrY"
                     /locus_tag="ACHLIF_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO9606314.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15958..17121)
                     /gene="emrK"
                     /locus_tag="ACHLIF_002310"
     CDS             complement(15958..17121)
                     /gene="emrK"
                     /locus_tag="ACHLIF_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO9606315.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            17537..18151
                     /gene="evgA"
                     /locus_tag="ACHLIF_002311"
     CDS             17537..18151
                     /gene="evgA"
                     /locus_tag="ACHLIF_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO9606316.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            18156..21749
                     /gene="evgS"
                     /locus_tag="ACHLIF_002312"
     CDS             18156..21749
                     /gene="evgS"
                     /locus_tag="ACHLIF_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO9606317.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(21805..22950)
                     /gene="yfdE"
                     /locus_tag="ACHLIF_002313"
     CDS             complement(21805..22950)
                     /gene="yfdE"
                     /locus_tag="ACHLIF_002313"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO9606318.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(23024..23968)
                     /gene="yfdV"
                     /locus_tag="ACHLIF_002314"
     CDS             complement(23024..23968)
                     /gene="yfdV"
                     /locus_tag="ACHLIF_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO9606319.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(24038..25732)
                     /gene="oxc"
                     /locus_tag="ACHLIF_002315"
     CDS             complement(24038..25732)
                     /gene="oxc"
                     /locus_tag="ACHLIF_002315"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO9606320.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(25786..27036)
                     /gene="frc"
                     /locus_tag="ACHLIF_002316"
     CDS             complement(25786..27036)
                     /gene="frc"
                     /locus_tag="ACHLIF_002316"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO9606321.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(27549..28181)
                     /gene="yfdX"
                     /locus_tag="ACHLIF_002317"
     CDS             complement(27549..28181)
                     /gene="yfdX"
                     /locus_tag="ACHLIF_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO9606322.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            28477..28752
                     /gene="ypdI"
                     /locus_tag="ACHLIF_002318"
     CDS             28477..28752
                     /gene="ypdI"
                     /locus_tag="ACHLIF_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO9606323.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(28829..29071)
                     /gene="yfdY"
                     /locus_tag="ACHLIF_002319"
     CDS             complement(28829..29071)
                     /gene="yfdY"
                     /locus_tag="ACHLIF_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO9606324.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            29424..30344
                     /gene="lpxP"
                     /locus_tag="ACHLIF_002320"
     CDS             29424..30344
                     /gene="lpxP"
                     /locus_tag="ACHLIF_002320"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO9606325.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            30700..30771
                     /gene="ypdK"
                     /locus_tag="ACHLIF_002321"
     CDS             30700..30771
                     /gene="ypdK"
                     /locus_tag="ACHLIF_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO9606326.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(30836..32074)
                     /gene="alaC"
                     /locus_tag="ACHLIF_002322"
     CDS             complement(30836..32074)
                     /gene="alaC"
                     /locus_tag="ACHLIF_002322"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO9606327.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         8794 a         7857 c         7445 g         8159 t
ORIGIN      
        1 ggaatacatg tatgaatccg tctgatgcca ttgaggcaat tgaaaaaccg ctctcctccc
       61 tgccttactc actttcccgt cacatcctgg aacatctgcg caaactcacc agtcacgaac
      121 ccgtgattgg cattatgggt aaaagcggag ccggtaaatc ctcactctgt aatgcactgt
      181 ttcaggggga ggtcaccccg gtcagtgatg ttcacgccgg cacccgggaa gtgcggcgct
      241 tccgtctgag tggccacggt cacagcatgg ttatcactga cctgcccggg gtgggcgaga
      301 gccgggacag ggatgcagag tatgaagccc tgtaccgtga cattctgccc gaactggacc
      361 tggtgctgtg gctgattaaa gccgatgacc gcgccctgtc tgtggatgag tatttctggc
      421 gacacatcct gcaccgggga catcagcagg tgctgtttgt ggtgacgcag gccgacaaaa
      481 cggagccctg ccatgaatgg gatatggccg gtattcagcc ttctcccgca caggcacaga
      541 atattcgcga aaaaacggag gcggtattcc gtctgttccg gcccgtacat ccggttgtgg
      601 ccgtatcggc ccgcaccggc tgggaactgg atacgctggt cagtgcgctc atgacagcgc
      661 ttcccgacca tgccgccagt cccctgatga cccgcctgca ggacgagctg cgcacggaat
      721 ctgtccgggg tcaggcccgt gaacagttta ccggtgcggt ggaccggata tttgacacgg
      781 cggagagcgt ctgtattgcc tctgttgcac gcacggttct gcgggccgtc cgtgactcgg
      841 tggtctctgt tgcccgcgcg gtatggaact ggattttctt ctgaacactc tgccagggga
      901 aatgcctgat tacattgcgt taaccaactg aaaacatgcc cccccctcat acccacctct
      961 ttctcttgcg tgacttccct gctgttaata gaataaaacg atcgataaaa caggtatcga
     1021 tcgttactat cgatcgttta tagtggttaa tggtctgcga aaaggcagct gaatctcttc
     1081 atcatgcaga acggaattgc acacaacgga ctgatacttc tctggcggat gagagagggg
     1141 aaacatttat gtccgggaaa aaccacttta tctgaccatg ctgttttgta cctgccggta
     1201 tccacttttg tgggtaccgg cttttttatt caccctctgt aaggaaaagc tgatgaaacg
     1261 acatctgaac accagctaca ggctggtatg gaatcacatt acgggcaccc tggtggtggc
     1321 ctccgaactg gcccgctcac ggggaaaacg cgccggtgtg gcggttgcgc tgtctcttgc
     1381 tgctgtcaca tcagtcccgg cactggctgc tgacacggtt gtacaggcgg gagaaaccgt
     1441 gaacggcgga acactgacaa atcatgacaa ccagattgtc ttcggtacgg ccaacggaat
     1501 gaccatcagt accggtctgg agtatgggcc ggataacgag gccaataccg gcggacaatg
     1561 gatacaaaat ggcggtatcg ccaacaacac tactgtcacc ggtggtggtc ttcagagagt
     1621 gaatgccgga ggaagcgttt cagacacggt tatcagtgcc ggaggcggac agagccttca
     1681 ggggcaggca gtgaacacca ctctgaacgg cggtgagcag tgggtacatg aaggcgggat
     1741 tgcaacggtt accgtcatta atgagaaggg ctggcaggcc gtcaaatccg gcgcaatggc
     1801 aaccgacacg gttgtgaata ccggcgcgga agggggaccg gatgcagaaa atggtgatac
     1861 cgggcagaac gtctacggag atgccgtacg caccaccatc aataaaaatg gtcgtcagat
     1921 tgtggctgct gaaggaacgg caaataccac tgtggtttat gccggcggcg accagactgt
     1981 acatggtcac gcactggata ccacgctgaa tggggggtac cagtatgtgc acaacggcgg
     2041 tacggcgtct ggcactgttg taaacagtga cggctggcag attatcaagg aaggtggtct
     2101 ggcggatttc accaccgtta accagaaagg caaactgcag gtgaacgccg gtggtaccgc
     2161 cacacatgtc accctgaaac agggtggagc actggtcacc agtactgcgg caacggttct
     2221 tggcagcaac cgtctgggca atttcacggt ggaaaacggt aaggctgacg gcgttgttct
     2281 ggagtccggc ggtcgcctgg atgtactgga aggccattca gcgcagaaaa cacgggtgga
     2341 tgacggcggt accctggcag tgtctgccgg tggtaaggca acagatgtca ccatgacatc
     2401 cggtggtgcc ctgatagcag acagtggtgc cactgttgag gggaccaatg ccagcggtaa
     2461 gttcagtatt gatggcatat ccggtcaggc cagcggcctg ctactggaaa atggcggcag
     2521 ctttacggtt aatgccgggg gacaggcagg caacaccact gtcggacatc gtggaacact
     2581 gacgctggct gccgggggaa gtctgagtgg cagaacacag ctcagtaaag gcgccagtat
     2641 ggtactgaat ggtgatgtgg tcagtaccgg cgatattgtt aacgcaggag agattcactt
     2701 tgataatcag acgacacagg atgccgtgct gagccgtgct gttgcaaaag gcgactcccc
     2761 ggtaacgttc cataaactga ccaccaccaa cctcactggt cagggcggca ccatcaatat
     2821 gcgtgttcgc cttgatggca gtaatacctc agaccagctg gtgattaatg gtggtcaggc
     2881 aaccggcaaa acctggcttg cgttcacaaa tgtcggaaac agtaacctcg gggtggcaac
     2941 caccggacag ggtatccggg ttgtggatgc acagaatggt gccaccacag aagaaggtgc
     3001 gtttgccctg agccgcccgc ttcaggccgg cgcctttaac tacaccctga accgtgacag
     3061 cgatgaagac tggtacctgc gcagtgaaaa tgcttatcgt gctgaagtcc ccctgtatgc
     3121 ctccatgctg acacaggcaa tggactatga ccggattctg gctggctccc gcagccatca
     3181 gaccggtgta aacggtgaaa ataacagcgt ccgtctcagc attcagggcg gtcatctcgg
     3241 gcacgataac aacggcggta ttgcccgtgg ggccacgccg gaaagcagcg gcagctatgg
     3301 cttcgtccgt ctggagggtg acctgctcag aacagaggtt gccggtatgt ctctgacgac
     3361 aggggtgtat ggtgctgcag gccattcttc cgttgatgtt aaggatgatg acggctcccg
     3421 cgccggcacg gtccgggatg atgccggcag tctgggcgga tacctgaatc tggtacacac
     3481 atcctccggc ctgtgggctg acattgtggc acagggaacc cgccacagca tgaaagcatc
     3541 atcggacaat aacgacttcc gcgcccgggg ctggggctgg ctgggctcac tggaaaccgg
     3601 tctgcccttc agtatcactg acaatctgat gctggagcca caactgcagt acacctggca
     3661 gggactctcc ctggatgacg gccaggataa cgccggttat gtgaagttcg ggcatggcag
     3721 tgcacaacat gtgcgtgccg gcttccgtct gggcagccac aacgatatga actttggtaa
     3781 aggcacctca tcccgtgaca ccctgcgcga cagtgccaaa cacagtgtgc gtgaattgcc
     3841 ggtgaactgg tgggtacagc cttctgttat ccgcaccttc agctcccggg gagacatgag
     3901 catgggtaca gccgcagccg gcagtaacat gacgttctca ccgtcccgga atggcacgtc
     3961 actggacctg caggccggac tggaagcccg tgtccgggaa aatatcaccc tgggcgttca
     4021 ggccggttat gcccacagtg tcagcggcag cagcgctgaa ggttataacg gtcaggccac
     4081 actgaacgtg actttctgat aatcaggtca cttcccttca ggccccggtc attatgaccg
     4141 gggcctttta ctttcaggga tcaacagata tgccgggttg tgcatcacgt ttattgcccg
     4201 aagacccttt tactgccaaa tggcatctga attgatcaca gtctggccag ttattacccg
     4261 atcccgatgg ggatattggg tgatctcctt ccatgaaaat acgcagctca ccagtggagg
     4321 gcttctcaga caataaacgt cattcatttc atctgtcagg ccgcgtcttc tccgggagac
     4381 gcggcttttt catttatacc gctaactcat tcataaggag caaataatgc gattagcaag
     4441 tcgttttggt catgtaaacc agatacgccg tgaccgcccg ctgacacatg aagaactgat
     4501 gcactatgtg cccagtattt ttggggaaga ccggcatacc tcccgcagtg aacgctatgc
     4561 gtacattccc accatcaccg tactagaaag cctgcagcgg gagggctttc agcccttctt
     4621 cgcctaccag atccgcgtgc gcgacccggg ccgccgggga tacaccaaac acatgctgcg
     4681 tctgcgccgt gacggggaga taaacggaca acatgtccct gaaatcatcc tgctgaactc
     4741 ccatgacggt acttccagct accagatgct gccgggttac ttcaggttcg tctgccagaa
     4801 cgggtgcgtc tgtggccagt ctctggggga agtgcgtgtt ccacaccggg gaaatgtggt
     4861 ggagaaagtt atcgaagggg cttacgaggt ggtgggcgtg tttgaccgga tagaggagaa
     4921 gcgtgatgcc atgcagtcgc tggtcctgcc gccaccgtca cgccaggcgc tggcacaggc
     4981 ggcactgact taccgttatg gtgacgaaca tcagcccgtc accaccgccg acattctgac
     5041 gccacgacgc cgggaggatt acggtaagga cctgtggagt gcttatcaga ccatccagga
     5101 gaatatgctg aaaggcggga tttccggtcg cagtgccaga ggaaaacgta tccatacccg
     5161 taccattcac agcatcgaca ccgacattaa gctcaaccgg gcgttgtggg tgatggcaga
     5221 aacactgctg gagagcatgc gctgatgcct tttccctgaa agcgcagtcc tgactcactt
     5281 ccgtccctgc ctccggacat tcttccattc atttactttt tataaggaat aatctcatga
     5341 caaccgttac gcataattcc accacacctt ctgtttccgt aaccgctgca tcagggaata
     5401 acccacctca gctggttgct actctcgtcc ctgatgaaca gcgcatcagc ttctggccgc
     5461 agcattttgg cctcattcca cagtgggtga ccctggagcc ccgtgtcttc ggctggatgg
     5521 accgtctgtg cgaagactac tgcggtggta tctggaatct gtacaccctg aacaacggcg
     5581 gggcatttat ggcacccgaa ccggatgacg atgatgacga aacatgggta ctgttcaatg
     5641 ccatgaacgg taaccgcgct gaaatgagcc cggaagccgc cggtattgcc gcctgtctga
     5701 tgacgtacag tcatcatgcc tgtcgtatgg agaattatgc catgacggtc cattattacc
     5761 ggttgcggga ttacgccctg cagcatccgg aatgcagcgc cattatgcgc atcatcgact
     5821 gaatggagag aagcacaatg caacaacttt cctttctgcc cggagagatg acgcccggcg
     5881 agcgcagcct cattcaacgg gccctgaaaa ccctggaccg ccatcttcat gaacccggcg
     5941 tggccttcac ctccacccgt gcagcacggg aatggctgat tctgaacatg gcgggactgg
     6001 agcgtgaaga attccgggtg ctgtatctga acaaccaaaa tcagctgatt gccggtgaaa
     6061 ccctcttcac cggcaccatc aaccgcacgg aagtccatcc ccgggaagtg attaaacgcg
     6121 ccctgtacca caatgccgct gccgtggtac tggcacacaa tcacccgtcc ggtgaagtca
     6181 cacccagtaa ggcagaccgg cttatcaccg aacgtctggt acaggcactg ggcctggtgg
     6241 atatccgggt gccggaccat ctgatagtcg gtggcaacca ggttttctcc tttgccgaac
     6301 atggtctgct ttaacccgtc acaaccacat cacacctgtt ttcactttta tcttctgtct
     6361 tcagaggtat cccattatga aaattatcac ccgtggtgaa gccatgcgta ttcaccaaca
     6421 acatccgaca tcccgtcttt ttccgttctg taccggtaaa taccgctggc acggcagtgc
     6481 tgaagcgtat accggtcgtg aagtgcagga tattcccggt gtgctggccg tgtttgctga
     6541 acgccgtaag gacagttttg gtccgtatgt ccggctgatg agcgtcaccc tgaactgagt
     6601 gggaattctg atgagcagaa ttatcgccac taccggacca ttcttagccg attttctgta
     6661 aggattttat cgtgtcagac acactccacg ggacaacact tcccgacgac aatcacgacc
     6721 gcccctggtg ggggctgccc tgcaccgtga cgccctgttt cggggcacgt ctggtgcagg
     6781 agggtaaccg gttgcattac cttgccgacc gcgccggtat cagaggccgg ttcagcgatg
     6841 cggatgcata ccatctggac caggcctttc cactgctgat gaaacaactg gaactcatgc
     6901 tcaccagcgg tgaactgaat ccccgccatc agcataccat cacgctgtat gcaaaagggc
     6961 tgacctgtga agctgatact ctcggcagtt gtagtttcgt ttatatggct gtttatgcta
     7021 cccgattctc aaatcaatta ccgaaagctt ctgttcatca actaactcat tacttttaat
     7081 gatggggccc tttctggggc ccaagaaatt aacaacgcta acaaacaaca taaataacaa
     7141 taaattaaat aaaaaatttc attcctgcag gggacaccag atacccttca aacgaaatct
     7201 accttcaccc cgtaaaagat aggtttggca gcacacttgc cttatatcta ctcattttta
     7261 ctgcaacagg ttgaaatctc agcactgtca gaaagcgctg atgactaaac agccctgggc
     7321 cgggcgatgt aaccatcaca cagaatcctg atagcgaaat atggcgtgac tcgatacttc
     7381 actccgcaat gcattccttg atgaattcgc aggaccgtga tacacgggac aggtcgctga
     7441 atgacgacaa tgtcctggaa atcagcgaac cgcgcatctg aagtacattt gagcgactgt
     7501 accagaacat gaatgaggcg tttggattag gtgattatta gcagggctaa gcactttagt
     7561 attattattt tccggttgag ggatagggag atatcgacaa caaccggaaa agtttacgtc
     7621 tatattgctg aaggtacagg cgtttccata actatttgct cgcgtttttt actcaggaag
     7681 aaaatgccaa atagcaacat caggcagaca atacccgaaa ttgcgaagaa aactgtctgg
     7741 tagcctgcgt ggtcaaagag tatcccagtc ggcgttgaaa gcagcacaat cccaagcgaa
     7801 ctggcaattt gaaaaccaat cagaaagatc gtcgacgaca ggcgcttatc aaaatttgcc
     7861 acgctgtatt tgaagacgga tatgacacaa agtggaacct caatagcatg taacagcttc
     7921 actaatgaaa taatccaggg gttaacgaac agcgcgcagg aaaggatacg caacgccata
     7981 atcacaacac cgataagtaa tgcatttttt ggccctaccc gattcacaaa gaaaggaata
     8041 atcgccatgc atagcgcttc gagtaccacc tggaatgagt tgagataacc atacaggcgc
     8101 gttcctacat cgtgtgattc gaataaacct gcataaaaga caggaaaaag ttgttgatca
     8161 aaaatgttat agaaagacca cgtccccaca ataaatatga cgaaaaccca gaagtttcga
     8221 tccttgaaaa ctgcgataaa atcctctttt tttacccctc ccgcatccgc cgctacgcac
     8281 tggtgatcct tatctttaaa acacatgttg atcatcataa atacagcgcc aaatagcgag
     8341 accaaccaga agttgatatg gggactgata ctaaaaaata tgccggcaaa gaacgcgcca
     8401 atagcatagc caaaagatcc ccaggcgcgc gctgttccat attcgaaatg aaaatttcgc
     8461 gccatttttt cggtgaagct gtcaagcaaa ccgcatcccg ccagataccc caggccaaaa
     8521 aagagcgccc ccagaattag acctacagaa aaattgcttt gcagtaacgg ttcataaacg
     8581 taaatcataa acggtccggt caagaccagg atgaaactca tacaccagat gagcggtttc
     8641 ttcagaccga gtttatcctg aacgatgccg tagaacatca taaatagaat gctggtaaac
     8701 tggttgaccg aataaagtgt acctaattcc gtccctgtca accctagatg tcctttcagc
     8761 caaatagcgt ataacgacca ccacagcgac caggaaataa aaaagagaaa tgagtaactg
     8821 gatgcaaaac gatagtacgc atttctgaat ggaatattca gtgccataat tacctacctg
     8881 tcgttaaaaa attcacgtcc tatttagaga taagagcgac ttcgccgttt acttctcact
     8941 attccagttc ttgtcgacat ggcagcgctg tcattgcccc tttcgccgtt actgcaagcg
     9001 ctccgcaacg ttgagcgaga tcgataattc gtcgcatttc tctctcatct gtagataatc
     9061 ccgtagagga cagacctgtg agtaacccgg caacgaacgc atctcccgcc cccgtgctat
     9121 cgacacaatt cacagacatt ccagcaaaat ggtgaacttg tcctcgataa cagaccacca
     9181 ccccttctgc acctttagtc accaacagca tggcgatctc atactctttt gccagggcgc
     9241 atatatcctg atcgttctgt gtttttccac tgataagtcg ccattcttct tccgagagct
     9301 tgacgacatc cgccagttgt agcgcctgcc gcaaacacaa gcggagcaaa tgctcgtctt
     9361 gccatagatc ttcacgaata ttaggatcga agctgacaaa acctccggca tgccggatcg
     9421 ccgtcatcgc agtaaatgcg ctggtacgcg aaggctcggc agacaacgca attgaacaga
     9481 gatgtaacca ttcgccatgt cgccagcagg gcaagtctgt cgtctctaaa aaaagatcgg
     9541 cactggggcg gaccataaac gtaaatgaac gttccccttg atcgttcaga tcgacaagca
     9601 ccgtggatgt ccggtgccat tcatcttgct tcagatacgt gatatcgact ccctcagtta
     9661 gcagcgttct ttgcattaac gccccaaaag gatcatcacc gacccgacct ataaatccac
     9721 ttattccgcc taatctggcg attcccaccg caacgttagc tggcgcgccg ccaggacaag
     9781 gcagtaggcg cccgtctgat tctggcaaga gatctacgac cgcatcccct aaaacccata
     9841 ctttggctga catttttttc ccttaaattc atctgagtta cgcatagtga taaacctctt
     9901 tctcgcaaaa tcgtcatgga tttactaaaa catgcatatt cgatcacaaa acgtcatagt
     9961 taacgttaac atttgtgata ttcatcgcat ttatgaaagt aagggactat atttttataa
    10021 aagttaacgt taacaattca ccaaatttgc ttaaccagga tgattaaaat gacgcaatct
    10081 cgattgcatg cggcgcaaaa cgcactagca aaacttcatg agcaccgggg taacactttc
    10141 tatccccatt ttcacctcgc gccacctgcc ggatggatga acgatccaaa cggcctgatc
    10201 tggtttaacg atcgttatca cgcgttttat caacatcacc cgatgagcga acactgggga
    10261 ccaatgcact ggggacatgc caccagcgac gatatgatcc actggcagca tgagcctatt
    10321 gcgctagcgc caggagacga taatgacaaa gacgggtgtt tttcaggtag tgctgtcgat
    10381 gacaatggtg tcctctcact tatctacacc ggacacgtct ggctcgatgg tgcaggtaat
    10441 gacgatgcaa ttcgcgaagt acaatgtctg gctaccagtc gggatggtat tcatttcgag
    10501 aaacagggtg tgatcctcac tccaccagaa ggaatcatgc acttccgcga tcctaaagtg
    10561 tggcgtgaag ccgacacatg gtggatggta gtcggggcga aagatccagg caacacgggg
    10621 cagatcctgc tttatcgcgg cagttcgttg cgtgaatgga ccttcgatcg cgtactggcc
    10681 cacgctgatg cgggtgaaag ctatatgtgg gaatgtccgg actttttcag ccttggcgat
    10741 cagcattatc tgatgttttc cccgcaggga atgaatgccg agggatacag ttaccgaaat
    10801 cgctttcaaa gtggcgtaat acccggaatg tggtcgccag gacgactttt tgcacaatcc
    10861 gggcatttta ctgaacttga taacgggcat gacttttatg caccacaaag ctttttagcg
    10921 aaggatggtc ggcgtattgt tatcggctgg atggatatgt gggaatcgcc aatgccctca
    10981 aaacgtgaag gatgggcagg ctgcatgacg ctggcgcgcg agctatcaga gagcaatggc
    11041 aaacttctac aacgcccggt acacgaagct gagtcgttac gccagcagca tcaatctgtc
    11101 tctccccgca caatcagcaa taaatatgtt ttgcaggaaa acgcgcaagc agttgagatt
    11161 cagttgcagt gggcgctgaa gaacagtgat gccgaacatt acggattaca gctcggcact
    11221 ggaatgcggc tgtatattga taaccaatct gagcgacttg ttttgtggcg gtattaccca
    11281 cacgagaatt tagacggcta ccgtagtatt cccctcccgc agcgtgacac gctcgcccta
    11341 aggatattta tcgatacatc atccgtggaa gtatttatta acgacgggga agcggtgatg
    11401 agtagtcgaa tctatccgca gccagaagaa cgggaactgt cgctttatgc ctcccacgga
    11461 gtggctgtgc tgcaacatgg agcactctgg ctactgggtt aacataatat caggtggaac
    11521 aacggatcaa cagcgggcaa gggatccgcg tcactcttcc cccttcacga ccttcaataa
    11581 tatgcaatgc agcttcccgc ccgataatgt catgtggaag ctgaattgtg gtcagcggcg
    11641 gtaaaaacag atgcccgacg ccaaccagat tatcaaagcc cattacggcg acatcctgcg
    11701 ggattcgtac ccccttcgcc agaagaacct gataagccac aaaggctgcg cgatcgttac
    11761 cacatatcag aacatcaaaa tctggtttgc ccgatttgaa gtggtcattg agtaaacttg
    11821 cgagatcggt gtagtgatca tcacctgttg ccatgtgaaa ttgtttcacc tcagccagat
    11881 ctcgtccagc atcacgccag gcctgctcaa atccctgccg acgataccct gttgccaacg
    11941 cactttccgg tagccagaag cataacggtt gacgatagcc cgccgcgagc aaatgctgtg
    12001 ttgattcata ttgtgcagtg taatcatcag ggatataact gggtaacgct gggtcatccg
    12061 ccacacagtt cgccaataca atattttcac catacagaga ctcaggcagc gtgatatgtc
    12121 gcagccccat tgtagtatag ataatgccat ccggacggtg ggcaagcagc tgacgtgccg
    12181 cgcgggcagc gtcatcttca gaaaaaatat tgattaaaaa actattccag ccgaactcgc
    12241 tggcggtttg ctcaatggca agcagaatat caacagagaa aggagtggta gccgtgtcct
    12301 gcgccagcac ggcgagagtc gacggcttac gtccttgagc gcgcatctta cgggcggaaa
    12361 gatcaggaac ataattcagg gtctggattg cctgcaatac gcggtcacgc gttgcaggac
    12421 gcacagattc tgcattatgc atcacccggg agactgtcat catcgacact cccgctaggc
    12481 gtgcgacatc ctttaatgaa gccataccca agccgtttgc cgtaaaacgg gcactgtagc
    12541 agaaacagac gtcactggcg agatccaacg atattctcaa cgccctatca cccgacacgg
    12601 caatacaata aaaaataaca ataattgccg gataattgta ctcaattccg cctctgttct
    12661 cgccaacgag taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg
    12721 gagaagaaaa tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa
    12781 attattgcga ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt
    12841 ttctggctag tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg
    12901 acagcgacat ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc
    12961 atctaagcgc aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt
    13021 atccgttggt aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga
    13081 cctcattggc tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc
    13141 atgcgcgctt atcccgtttt gcaccctatc tggcaaaagc atttcctgaa actgctgcca
    13201 ctggggggat tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa
    13261 aagaatatca gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca
    13321 tttccggctc cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac
    13381 tggctctgga agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg
    13441 agtttaaaca gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt
    13501 tatcaatcgg cattatgagc gcccgcattg gctttaagat gacagttcat atgtctgctg
    13561 atgcccgggc atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg
    13621 agcaagatta tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact
    13681 gtttctttat tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc
    13741 agcgtcttaa agcgcaattt gcccagcaag gtcgtatcgt cgatgctgat aaccctctgt
    13801 ttgtctatct gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc ggacttaaac
    13861 tggcgtttgg cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt
    13921 tgttaggcgt ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca
    13981 accttaccgc agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa
    14041 tggagcgtct gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg
    14101 gctggctggc gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg
    14161 gacctcagcg cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac
    14221 aattgcgtaa tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag
    14281 agatgaatca atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct
    14341 ttccctgggt gagcgatgct gccgatggcg cagacttaag atccccgctc ttacccgcta
    14401 taaccccctc cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc
    14461 aaaccaaaac cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag
    14521 aaatagaaag tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg
    14581 aaccatagta tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga
    14641 tggttgctgt caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca
    14701 acgacgtacc aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg
    14761 caaatttatt atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg
    14821 caacggcgaa tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca
    14881 taaatgtcac agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta
    14941 acaaccgcat gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca
    15001 tgatgccgat gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct
    15061 ggagtaactg cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac
    15121 tcacaatacc aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct
    15181 ctgaggtcga ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata
    15241 ttattattgt actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca
    15301 agccaccaac accgagcact aacagggtca gtcctggtag attcattttg accggtgaag
    15361 tttcagtttc tcttccttta agtaaggtta agcataatgt caggacgata atccccatag
    15421 ggacattgat taaaaatatc caaccccagc taaagttatc gcaaatataa ccgcccaata
    15481 tcggcccaca tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc
    15541 ttttttctgg tggataattc cttaataata aactctgtga cagtggaatt aacggccccg
    15601 ccattaatcc ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac
    15661 acattaagga agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta
    15721 ttctttgtgc cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg
    15781 tgataaccca ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg
    15841 cgacgttaga aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag
    15901 taacgcacca taacgtccca ccggttaatg gtgccggagt cgatttagtg attgccatta
    15961 aagttgtcca ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat
    16021 aactaaagcc ttactggtat aagccggcat ggacgtcacg gttgaagcca gatcaggcat
    16081 ctcagcaatg tcttcgttct tcgtatcaat agttgcagtc atcgataaac caatgcgtaa
    16141 ggggtgttcc atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat
    16201 tttgatccag ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc
    16261 catattgatc cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat
    16321 aatattgact gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt
    16381 aacccacatt tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc
    16441 gccgacctga acactccgct gggcaatata gccagtaacc ggactcctta tatccgtacg
    16501 tttaagcgcc aaccaggctt ctttagttgc atccgccgct tccacgactt gtggctgacg
    16561 gtttaatggt gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt
    16621 cagtgccgct ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat
    16681 aaccccctgc ttcgctaacg gtactcgacg gttataatct tctaaagatt gttgatactg
    16741 aatacgtgct gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt
    16801 ttgccgaaca atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt
    16861 tttatccagt gaaactaaaa tgtcaccttg tcgaacgtag ttcgtatctt tatgattaac
    16921 gacagtgaca ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc
    16981 gtcatctgta ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa
    17041 cgcaataaat aaaactactg ccaataaaga aaagtatttt cttctgttag aatgtttttt
    17101 atttgaatta atctgttcca ctattatctc tcatttctca tagatgaaat ttatgaattg
    17161 aatgaaaaga gcattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag
    17221 gaacgttgga ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg
    17281 cttttgcaat acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg
    17341 aaactataaa tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact
    17401 atttatatgg tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt
    17461 gagaaaatga gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt
    17521 gcaaagggaa taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc
    17581 aattcgtaat ttattgatca aaaacgatat tgaaatctta gcggagttga ctgaaggggg
    17641 aagcgccgtt cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat
    17701 ccccggagtt aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat
    17761 tattattatt gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg
    17821 cgctaatgga ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc
    17881 tgcaaaaaat ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac
    17941 gtccgaccag caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat
    18001 tcttgatggc aaggataata atgacattgc tgaaaaaatg ttcatcagca acaaaactgt
    18061 cagcacttat aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta
    18121 cacattcgcg caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc
    18181 ttctctgttg tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc
    18241 gtggcatcag tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat
    18301 tacgttggtt agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta
    18361 ccttgttgca taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc
    18421 ttttaaaaag agcgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag
    18481 caatggacgc gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc
    18541 cgcctcttaa tagtgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg
    18601 taaccaccct tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc
    18661 gggtagcaaa ttacccccca gacgaggtaa ttcatcaatc gtttccaaaa gcaacaatta
    18721 tctcttttac aaatttatat caggcattag catccgtatc agccggacag aatgattact
    18781 ttattggtag taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa
    18841 atgtagtgaa atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat
    18901 ccgtcattct taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct
    18961 atgaagtttc acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag
    19021 aactcactga acatgaaaaa cagtggatta agcaacatcc cgatttaaag gtgctggaaa
    19081 atccttactc tcctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg
    19141 gggatattct taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt
    19201 cacacaatat ccatgccgga acacagctta accccggtgg atgggatata atacctggcg
    19261 ctatttatag tgaagatcga gaaaataatg tttcatttgc agaagctttc ataacaacgc
    19321 catacgtttt tgtcatgcaa aaagcacctg acagtgaaca aacattaaaa aaaggaatga
    19381 aagttgccat tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg
    19441 ttgaatggat acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac
    19501 ttgatgctct ggtcgcgaca cagttaaatt cgcgttacat gatcgatcat tactatccta
    19561 atgaacttta tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc
    19621 ctcgcggaga accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa
    19681 gcgaagttct gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat
    19741 gggacctata tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca
    19801 gtagcctttt atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg
    19861 gtgatttaga aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa
    19921 cttatgttgt aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt
    19981 tcactgcgga ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta
    20041 aagatgtttt ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat
    20101 acacacaggt atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata
    20161 cattatgcaa tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta
    20221 ctgaaacgcg tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta
    20281 ccgtagcaaa aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct
    20341 ctattatggg cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg
    20401 aggcgatttc acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc
    20461 ttgatgtcga caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc
    20521 ctactttagt ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg
    20581 cattaagttg cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt
    20641 ttaagcaggt cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag
    20701 taaaaattac gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga
    20761 ttatggattc tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca
    20821 gccaaacaag tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag
    20881 aattaattaa aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa
    20941 catttacgat cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag
    21001 cagaacaccc catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga
    21061 ccaacaggct attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca
    21121 ctgatggtgt gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg
    21181 acgttaatat gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt
    21241 cttccttacc catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt
    21301 taagttgcgg catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac
    21361 atttaagtca gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg
    21421 aagccctgaa aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt
    21481 tccagcatga aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata
    21541 acagaacttt ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc
    21601 aaaagttgat taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc
    21661 ctgaaattct tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga
    21721 ttgctgtttt ctgtcagaaa aatgactaaa tagcggctcc cacaatgttc aaatgtggga
    21781 gctatttacc agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg
    21841 ctggtcgagc gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat
    21901 cggattaccc ggcatcatta ttcccccggc ttcaatcaac atatttctcg cctgagtttg
    21961 tggcaattta atggcctcag ccacacttaa taacggcgcg acgggtacac caacttcatg
    22021 tattctggct aaccaaactt cagctgcctg cgtttttaac gtccgctcaa tatattgttt
    22081 aagaatagcc tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag
    22141 ttccgtaagc tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat
    22201 cgtaatcggc ttatcctgag tattgaaaac atcaaaaggt gccatgtagg gatggcgatt
    22261 tcccagacgt tgtggtgact tccctgtcgc gatatatgcc atcagaccat gctccagaaa
    22321 actcagcgtg gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg
    22381 gccataaagt gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag
    22441 agatgtacca acgcgcactg gcggagcatc aggatatcct gtttccatca ttatcccgct
    22501 cattgcctga atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa
    22561 acctgacgat gaagcatata tgaggcgcgg gttgatttct tgaagcgttt cccatgaaaa
    22621 ccccagtttt tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag
    22681 catatttata aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt
    22741 gccatgatta ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg
    22801 ggtatcatca ccatgacccg gcggctcaac tttaattacc cttgcgccca tattacaaag
    22861 aagttgagtt ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc
    22921 aaacggccct ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg
    22981 aaaagacact tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac
    23041 tgacataaat ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg
    23101 ctgtaccagt acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg
    23161 gcggtaatgc gcctgccagt accatcatct gcagatgttc gctgttcaaa tgacatgcca
    23221 taccaacgag aagcagtgcc agtggcatca gaatcagctt caggaaggtg ttataagcaa
    23281 tttcggcact gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta
    23341 cccctgagtt agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta
    23401 ccccaaccaa caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg
    23461 cagaaattaa tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca
    23521 gcaaatacag accaatagga atagtaattg cgttaacaat aatagaaata attgctacca
    23581 ctaaacctgt tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc
    23641 caatggtagg tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa
    23701 aaaatttgta gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata
    23761 caagggtcag acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa
    23821 atagagccgc aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt
    23881 ctgaaaaagt ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca
    23941 ataaatcgcc aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat
    24001 cgcataatga gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg
    24061 ggttaagttt ggtaatatgg ccactttcag ttcctgctgc agggtcgatg accacattaa
    24121 taatggtcgg tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg
    24181 tggtgacgtt atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt
    24241 gcaacagatc cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt
    24301 agatgccgcc attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt
    24361 caatttccat cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac
    24421 cagaagtcac gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac
    24481 cacaatccag acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg
    24541 tatttgcacc ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag
    24601 cacttaatgc attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt
    24661 tttgtgcatt ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa
    24721 atgtgttttg tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga
    24781 ctggcacagc aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa
    24841 actgtgtatc tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac
    24901 caacaagcat gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa
    24961 gtggatgcgt atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag
    25021 tactttcaat aaactcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa
    25081 ggataattaa tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg
    25141 ggcatggcaa taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt
    25201 ctttttccat cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc
    25261 gacccgatac agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat
    25321 taacgcgaaa tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat
    25381 aatcaccttg ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg
    25441 gaaaaccatt taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag
    25501 aaactgtcag gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc
    25561 ctgccgactg ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg
    25621 ccatatccgt cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta
    25681 atgcttcaac gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata
    25741 acctcgaaat ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt
    25801 ttgcttcatt gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt
    25861 atgttcacct aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg
    25921 acaaccaacg gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact
    25981 ttggcgcaaa gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat
    26041 atcaaactga gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc
    26101 gatttcagca aaaatatcga aaatatgtgg ctgtcgtgca tgggctgtac tgtatgccgg
    26161 atcggtaatc cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg
    26221 aatagtgaaa taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca
    26281 gccaggctga ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc
    26341 attcggatac tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg
    26401 taatttcacg cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc
    26461 acgccccgtt ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc
    26521 ggtgttgctg tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt
    26581 agtggatgcc gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata
    26641 aggcgaacac tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg
    26701 aatatgctcc caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa
    26761 gatatcagct tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt
    26821 taactcaata gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg
    26881 aatatctcgc agctggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac
    26941 gtcagcgcca aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa
    27001 atcgagaact ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa
    27061 tatgtgttaa tttagcgttt atttaaggcg taatatcttc tataaataac ccgcaaaata
    27121 tcgtcgtagc tgcactcgat atttttaaaa tcctgtatca agtgctgaaa atcatttcat
    27181 cgccacttcc aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa
    27241 atattgattc gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt
    27301 aagaagcgcc ggtgatttac aagtaagggt atgattacgc tttttaaagc gtaccataaa
    27361 acataacata ataaatgatt tgtgatgaca cgggattatt gggatttact ttgaggaaga
    27421 taaaatgcgt gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta
    27481 ttgtggtcat aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc
    27541 tgtcagcatt agttcacaaa cagtgcttcg ctgtcgacga tgattccatc ttccgcacct
    27601 ttaagcgcca ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca
    27661 agtgcattac gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg
    27721 gccaggcgaa gttcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca
    27781 atttttatcg cggcttcttt ttcgggagtt gctacataac tctcagaaat tccgacagat
    27841 gcattaatga caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac
    27901 tttgcccatt cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt
    27961 gccttttctg gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca
    28021 tataaacctt gttcagagat acgctctgct gctgccgttt tctgggtttg ctgaacttgc
    28081 tgagtttgct gttgtgccgc taccggtgca ttatcagcag cccatacagt tgaagatgcc
    28141 agaattgctg tgaccatcgt agccataatt aaacgtttca tacttacctc attctttaac
    28201 agttttagaa aacgccgcca tcatattggg tgagcatgaa atgagaaatc agagagcaac
    28261 ggtcattttg aaagggttat cttacagttg tagttattat cgcaagaagt attttagtct
    28321 tacaattgta ggaatctgct tcctgtcgca gagaactatt cttatgtacc acaaagagat
    28381 gtcatttatt atatcgatgt aaatattgat taattatatg taaatacagc cataacatcg
    28441 taggttctca gtgaaccatt ttcaggataa ctttctatga aagtaaactt aatacttttc
    28501 agcttatttt tattggtctc tattatggca tgcaatgttt ttgcattttc catttctggt
    28561 ggtgtaagtg agaggagcta taaagagact gaaaaaacat cagcgatgac aaccacacac
    28621 tctacaaaac ttcagccatc acaggcgatt ttgtttaaga tgagagaaga tgcgccacca
    28681 ttaaacctca cagaagaaat cacgcctact tatccgacaa aggcgaatta tcttattcat
    28741 cctgtgcgat agcgtctgtc acatggcagg tgaacaatca tgtctgatgc tgatgtgaac
    28801 acctgcccaa atgagccatc ctgacccact atcgcctgcc ataccccacc ataaagcgaa
    28861 tcatcaagcc acaagccggg gcaagaaaca taaatatccc cgtcactagt tcattaccgc
    28921 cagttaacca catggtgaag taataaatga gtgcagcgag gatcaccatg ccgaagaaag
    28981 aagagaccgc cgataccacg ttcagtacct gaccgatgta gccgcttacg cagacaatac
    29041 acagggcgag aaacatccat aaataaatca tagctcaacc tcctgccgcg ctaaaaaacc
    29101 tgtaacttca gtcactttaa tagtaagaca atccttaacc tctggcaatt tttgctaagc
    29161 atcaatgaaa acagcatgtt aaatgcaaga ctgttgtgta cggaaaaata tttactttgc
    29221 acgattaata atcagtagct gaaagcagtc agcgagtgat aaagtacaga taccgcattc
    29281 atgctcctcg tggttatgtc ctgactagtc tttacactct ttacaggaac cattgtcgta
    29341 catgatggcc caaccaattg agtgtaccgc tgtcgacatg gaatcttctg cgatacaaca
    29401 attcgtatct acagaaggta actatgtttc cacaatgcaa attttcccgc gagtttctac
    29461 atcctcgcta ctggctcaca tggtttgggc ttggtgtact ctggctttgg gtacagcttc
    29521 cttatcctgt tctctgcttt ctcggcacgc gtattggcgc aatggcgcga ccattcctga
    29581 aacgtcgtga atctatcgcc cgtaaaaacc tggaactttg tttcccgcag cattctgcgg
    29641 aagaacgcga gaagatgatt gccgaaaact ttcgttcact cggcatggcg ctggtagaaa
    29701 ccggcatggc atggttctgg cccgacagtc gcgtacgtaa atggtttgat gttgaagggt
    29761 tggataacct taaacgcgca caaatgcaaa atcgcggcgt aatggttgtc ggcgtccatt
    29821 ttatgtcgct ggaactgggc ggccgcgtga tgggactgtg ccaaccaatg atggctacct
    29881 atcgtccaca taataatcag ctgatggaat gggtgcagac ccgtgggcgc atgcgctcta
    29941 acaaagcgat gatcggcaga aataatctgc gcggcattgt cggtgcactg aagaaaggtg
    30001 aagcggtatg gtttgctccc gatcaggatt atggtcgtaa aggcagctcc ttcgcgccgt
    30061 tctttgcggt ggaaaatgtc gccacaacca atggcaccta tgttctctcc cgtctctctg
    30121 gcgcagccat gttgaccgta acgatggtaa gaaaagcgga ttacagcgga tatcgtttgt
    30181 tcatcacccc agagatggaa ggctacccga cagatgaaaa tcaagccgct gcctatatga
    30241 acaagattat cgagaaagag atcatgcgcg caccggagca gtacctctgg atccaccgtc
    30301 gctttaaaac gcgcccggtg ggagaatcgt cgttgtacat ttaacaaact gctcttactc
    30361 tttccctcct caaatcggcc ataataacct catggttttt atggcttttt tcttaaggtt
    30421 ttttccttcg ctgaatatct aattattacc gtcagaaatt aaactgtcgc cgatatgaga
    30481 cacttgtaag ttactaaaag taaataagaa aatgtctctt gtctcgtttc agttttaggc
    30541 gtacattagc gccgtctcga gcagtttagc tcagaattct ggggtgatgc ccacggtaaa
    30601 atacagggct tttaaggtcg ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag
    30661 tcattcaggc gtaacagtaa ttacgcggag agatgtaaag tgaaatattt ctttatgggc
    30721 atttctttta tggtcatcgt ttgggccggt acttttgccc tgatgatcta aaagcaaact
    30781 gtgcaatgaa aaacaggagc catcaggctc ctgtttgcat ttctgacgac ggtgtttatt
    30841 ccgcgttttc gtgaatatgt ttgctgctgg cgggtagtaa accgtcggca cggaacatcg
    30901 ctttaatccc acgaatcgcc tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa
    30961 catgcgtatc accatagtcg ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt
    31021 taaggagctt cttggcaaat tccagcgatc ccatggccgc atatggttcc gggattttcg
    31081 cccagacata catcgaagcc ttcggcattt caaccatcca gcccgcttca tgcagccctt
    31141 taaccaatac atcacggcgg cgtttgtact gttcggcaat gtcgcgcacg cactgttgat
    31201 cgccctccag cgccgcaatc gccgccacct gcaacggcgt aaaggtgccg taatcgtgat
    31261 agcttttaat acgagccagg gcgctgacca gcgttttgtt gccaaccata aagccgatac
    31321 gccagcccgc catgttgtag cttttcgaca gcgtaaagaa ctcgactgcc acatcgcgtg
    31381 cacccggtac ctgcatgatt gacggcgctt tccagccatc gtagacgata tcggcatagg
    31441 ccaggtcgtg gaccaccagc acatcgtagc gtttcgccag cgccacaacc ttttcgaaga
    31501 actccagctc cacgcattgc gcggttggat tcgacgggaa gccgaggatc atcatcttcg
    31561 gtttcggata actttcacga atggcgcgct ccagttcgtt gaagaaatcg acaccttcca
    31621 ccagcggcac tgagcgtacc tgcgccccgg caatcaccgc gccataaata tggatcgggt
    31681 aacttggatt cggcaccagc accgtgtcac catgatccag cgtcgccagc atcagatgcg
    31741 ccaggccctc tttcgaacca atagtgacga tggcttctga ttccgggtcg atttcaacgt
    31801 cgtagcgatc ctgataccag cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag
    31861 tggagtaacc atgcgtgtcc gggcgctggg ccacagtaca taatttttcg acgatatgcg
    31921 gcggagtcgc accgtccggg ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac
    31981 gcgcagccat tttcagttca gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc
    32041 gcgtaaagcg acgttcaggg cgagtgtcag ccataatttc ctcagattaa cgttagcgcc
    32101 cggaccgtcc gagcgacgct gccacaatgg tggctgtttt gaaaatagcc tgattaattt
    32161 ctgtctgtcc agcgttttag aacattttta tcaatggctt taaatgagga actccacttc
    32221 aaaaacagga aatttttcta cgctaacaag aaaat
//
LOCUS       DAZGOB010000047        31911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGOB010000047 DAZGOB010000000
VERSION     DAZGOB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.047"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..301
                     /locus_tag="ACHLIF_002323"
     CDS             2..301
                     /locus_tag="ACHLIF_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606328.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(348..1235)
                     /gene="rarD"
                     /locus_tag="ACHLIF_002324"
     CDS             complement(348..1235)
                     /gene="rarD"
                     /locus_tag="ACHLIF_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO9606329.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1287..1754)
                     /gene="yigI"
                     /locus_tag="ACHLIF_002325"
     CDS             complement(1287..1754)
                     /gene="yigI"
                     /locus_tag="ACHLIF_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO9606330.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1919..2788
                     /gene="pldA"
                     /locus_tag="ACHLIF_002326"
     CDS             1919..2788
                     /gene="pldA"
                     /locus_tag="ACHLIF_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO9606331.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2921..4750
                     /gene="recQ"
                     /locus_tag="ACHLIF_002327"
     CDS             2921..4750
                     /gene="recQ"
                     /locus_tag="ACHLIF_002327"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO9606332.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4814..5434
                     /gene="rhtC"
                     /locus_tag="ACHLIF_002328"
     CDS             4814..5434
                     /gene="rhtC"
                     /locus_tag="ACHLIF_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO9606333.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5496..6116)
                     /gene="rhtB"
                     /locus_tag="ACHLIF_002329"
     CDS             complement(5496..6116)
                     /gene="rhtB"
                     /locus_tag="ACHLIF_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO9606334.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6227..7249
                     /gene="pldB"
                     /locus_tag="ACHLIF_002330"
     CDS             6227..7249
                     /gene="pldB"
                     /locus_tag="ACHLIF_002330"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO9606335.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7257..8057
                     /gene="yigL"
                     /locus_tag="ACHLIF_002331"
     CDS             7257..8057
                     /gene="yigL"
                     /locus_tag="ACHLIF_002331"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO9606336.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8133..9032
                     /gene="bioP"
                     /locus_tag="ACHLIF_002332"
     CDS             8133..9032
                     /gene="bioP"
                     /locus_tag="ACHLIF_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO9606337.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8920..9873)
                     /gene="metR"
                     /locus_tag="ACHLIF_002333"
     CDS             complement(8920..9873)
                     /gene="metR"
                     /locus_tag="ACHLIF_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO9606338.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9991..12252
                     /gene="metE"
                     /locus_tag="ACHLIF_002334"
     CDS             9991..12252
                     /gene="metE"
                     /locus_tag="ACHLIF_002334"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO9606339.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12291..13106)
                     /gene="ysgA"
                     /locus_tag="ACHLIF_002335"
     CDS             complement(12291..13106)
                     /gene="ysgA"
                     /locus_tag="ACHLIF_002335"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO9606340.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13368..14129
                     /gene="udp"
                     /locus_tag="ACHLIF_002336"
     CDS             13368..14129
                     /gene="udp"
                     /locus_tag="ACHLIF_002336"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO9606341.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14270..15697
                     /gene="rmuC"
                     /locus_tag="ACHLIF_002337"
     CDS             14270..15697
                     /gene="rmuC"
                     /locus_tag="ACHLIF_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO9606342.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15792..16547
                     /gene="ubiE"
                     /locus_tag="ACHLIF_002338"
     CDS             15792..16547
                     /gene="ubiE"
                     /locus_tag="ACHLIF_002338"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO9606343.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16561..17166
                     /gene="ubiJ"
                     /locus_tag="ACHLIF_002339"
     CDS             16561..17166
                     /gene="ubiJ"
                     /locus_tag="ACHLIF_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO9606344.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17163..18803
                     /gene="ubiB"
                     /locus_tag="ACHLIF_002340"
     CDS             17163..18803
                     /gene="ubiB"
                     /locus_tag="ACHLIF_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO9606345.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18882..19151
                     /gene="tatA"
                     /locus_tag="ACHLIF_002341"
     CDS             18882..19151
                     /gene="tatA"
                     /locus_tag="ACHLIF_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO9606346.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19155..19670
                     /gene="tatB"
                     /locus_tag="ACHLIF_002342"
     CDS             19155..19670
                     /gene="tatB"
                     /locus_tag="ACHLIF_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO9606347.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19673..20449
                     /gene="tatC"
                     /locus_tag="ACHLIF_002343"
     CDS             19673..20449
                     /gene="tatC"
                     /locus_tag="ACHLIF_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO9606348.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20491..21273
                     /gene="tatD"
                     /locus_tag="ACHLIF_002344"
     CDS             20491..21273
                     /gene="tatD"
                     /locus_tag="ACHLIF_002344"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO9606349.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21270..21758)
                     /gene="rfaH"
                     /locus_tag="ACHLIF_002345"
     CDS             complement(21270..21758)
                     /gene="rfaH"
                     /locus_tag="ACHLIF_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO9606350.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21925..23418
                     /gene="ubiD"
                     /locus_tag="ACHLIF_002346"
     CDS             21925..23418
                     /gene="ubiD"
                     /locus_tag="ACHLIF_002346"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO9606351.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGVLDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23464..24165
                     /gene="fre"
                     /locus_tag="ACHLIF_002347"
     CDS             23464..24165
                     /gene="fre"
                     /locus_tag="ACHLIF_002347"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO9606352.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24357..25520)
                     /gene="fadA"
                     /locus_tag="ACHLIF_002348"
     CDS             complement(24357..25520)
                     /gene="fadA"
                     /locus_tag="ACHLIF_002348"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO9606353.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25530..27719)
                     /gene="fadB"
                     /locus_tag="ACHLIF_002349"
     CDS             complement(25530..27719)
                     /gene="fadB"
                     /locus_tag="ACHLIF_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO9606354.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALKRPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27909..29240
                     /gene="pepQ"
                     /locus_tag="ACHLIF_002350"
     CDS             27909..29240
                     /gene="pepQ"
                     /locus_tag="ACHLIF_002350"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO9606355.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29240..29854
                     /gene="yigZ"
                     /locus_tag="ACHLIF_002351"
     CDS             29240..29854
                     /gene="yigZ"
                     /locus_tag="ACHLIF_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO9606356.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29893..31344
                     /gene="trkH"
                     /locus_tag="ACHLIF_002352"
     CDS             29893..31344
                     /gene="trkH"
                     /locus_tag="ACHLIF_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO9606357.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFLVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31356..31901
                     /gene="hemG"
                     /locus_tag="ACHLIF_002353"
     CDS             31356..31901
                     /gene="hemG"
                     /locus_tag="ACHLIF_002353"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO9606358.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7383 a         8114 c         8745 g         7669 t
ORIGIN      
        1 tatgggaaat ggtaactttt ggcagttgtt cttgggtaaa gaaccttaca gcaaaaaatc
       61 tgattttgtt aaggcatggt tattgtggtg ggctattacc atccttgttc tatttatttt
      121 aagcctgact acgacattaa atttaagaag tataggtata gggttaagtt ttgggttttg
      181 tcaaatcctt ggtagatata cgatgtctct aggatggagt aagtactggg gagttttggg
      241 ggtatttcct gtaacaaata ttgttctttt tcttatctta atttttttca aaaaaagata
      301 aataagtcag tgggtaggct ctttaaacgg cctacccatg ttaattctta actttttcta
      361 cgctgcgtat aaatcgcatc catcacaaaa attgccagcg ccacccaaat aaaggcgaaa
      421 gtcaccatct tatcggcacc cggtttttca ccataaaacg tcacagccag caggaacatc
      481 agcgtcgggc caatgtactg gaaaaagcct aacgttgaga gacgcaagcg cgtggcagcg
      541 gcggtaaaac acaacagcgg tacggtagtg acaataccgg cggcgatcag cagtaaattc
      601 agcgacatcg ggttttgccc catatggctg gttgagctgt cggcaatagc aaacaggtaa
      661 attgccgcca cgggcagcag ccacatggtt tcgattaaca tgccggtttg cgcttcaacg
      721 gcaatcttct tgcgtaccag accgtagaag gcaaaactaa atgccagtcc cagcgcgata
      781 ataggtagcg aaccaaaagt ccacagctgg actaacacgc cacatatcgc cagaatcacc
      841 gccagccatt gcatccggcg gaatcgctcg ccgaggaaaa tcatccccag cacaatgttc
      901 accagcgggt taataaagta accaaggctc gcttccagca tatggtgatt gttcaccgcc
      961 cagataaaca gtagccagtt gccaccaatc agcacggcag agactgccag cataaaaatt
     1021 ttctgtggcg tctgaatcag cgtttttaaa taggaccact ggcggcaaat gctcatcagc
     1081 accaccataa agaaaaacga ccagatcacg cgatgcgtca ggatttcatc ggcaggcacg
     1141 tagtaaatca acttgaagta cgctggcgct ataccccaaa taaaataagc ggcaagagcg
     1201 agtaatacgc cctgccgcgt ttgttttgca tccatcggga atactcattt ttaattggta
     1261 acagcagttt acctgctttt atgtcttcaa cctaccatat aggtggcggt ggcactggca
     1321 atataaagct gttcttcatt gtgtaattca acgcgggcga cggcgacttt attgcctgca
     1381 cgcaacaggc tactggtagc agtaaaacgc tcgcccctgc ctgggcgcag ataatcaacg
     1441 cgaagatcaa tggtccccat ccgcgatagc cgctggcgta gttcatcttc actgatggtt
     1501 tcgtggcggg ttaaggtact tcccacgcac accagacctg cggcgacatc cagcgctgac
     1561 gcaataaccc cgccgtgcaa aatgctttgc gcccagttac ccaccatcat tggctgattt
     1621 ttaaaggcca gctgtgcgaa ctctttttcg taacgctcca gctccatccc taacgcgcgg
     1681 ttaaatggca tgtgataaac aaacatctca cccactaatt tcagggcttg ttcagcggtc
     1741 agtacggcag acatatcatc cttacacttc attggttaat gaaatgttga ttttatgctt
     1801 ctttgttgtt ggtttctact ttaggaaggg ataactaacg gctatggagt taagtatgta
     1861 aaatagcccg cagaaaaata ttcaccttat caataattcg ttacggagaa cacgaccgat
     1921 gcggactctg cagggctggt tgttgccggt gtttatgttg cctatggcag tatatgcaca
     1981 agaggcaacg gtgaaagagg tgcatgacgc gccagcggtg cgtggcagta ttatcgccaa
     2041 tatgctgcag gagcatgaca atccgttcac gctctatcct tatgacacca actacctcat
     2101 ttacacccaa accagcgatc tgaataaaga agcgattgcc agttacgact gggcggaaaa
     2161 tgcgcgtaag gatgaagtaa agtttcagtt gagcctggca tttccgctgt ggcgtgggat
     2221 tttaggcccg aactcggtgt tgggtgcgtc ttatacgcaa aaatcctggt ggcaactgtc
     2281 caatagcgaa gagtcttcac cgtttcgtga aaccaactac gaaccgcaat tgttcctcgg
     2341 ttttgccacc gattaccgtt ttgcaggttg gacgctgcgc gatgtggaga tggggtataa
     2401 ccacgactct aacgggcgtt ccgacccgac ctcccgcagc tggaaccgcc tttatactcg
     2461 cctgatggca gaaaacggta actggctggt agaagtgaag ccgtggtatg tggtgggtaa
     2521 tactgacgat aacccggata tcaccaaata tatgggttac taccagctta aaatcggcta
     2581 tcacctcggt gatgcggtgc tcagtgcgaa aggacagtac aactggaaca ccggctacgg
     2641 cggcgcggag ttaggcttaa gttacccgat caccaaacat gtgcgccttt atactcaggt
     2701 ttacagcggc tatggcgaat cgctcatcga ctataacttc aaccagaccc gtgtcggtgt
     2761 gggggttatg ctaaacgatt tgttttgatg aacggttgag tggttggcaa atctggaacc
     2821 cagcatccag gattaccctc tcagagacta aaagcattgc agtttctcgc gcaggcgctg
     2881 aaaatagcgc ctgtttttat ttcaggcaat cggggtgaat gtggcgcagg cggaagtgtt
     2941 gaatctggag tccggagcta aacaggtttt acaagaaacc tttggctacc aacagtttcg
     3001 ccccggccag gaagaaatta tcgacactgt gctttccggt cgcgattgcc tggtcgtcat
     3061 gcccaccggt ggcggaaaat ccctttgcta tcaaattcct gccttattgc taaacggcct
     3121 taccgtggtt gtttcaccgc tgatttcgtt gatgaaagat caggtggatc aactgcaagc
     3181 caacggcgtg gcggcggcgt gccttaactc gacgcaaacc cgcgaacagc aacttgaagt
     3241 gatgacaggc tgccgcaccg ggcaaattcg tctgctttat atcgccccgg aacgcctgat
     3301 gctggataac tttcttgagc atctggcgca ctggaatccg gtgttattag ccgttgatga
     3361 agcgcactgt atctcccaat ggggccacga tttccgcccg gaatatgccg cgctcggtca
     3421 gttgcgccag cggttcccga cgctgccgtt tatggcgctg accgccacag ccgacgacac
     3481 cacgcgccag gatatcgtgc gcctgctggg gctgaacgat ccgctgattc aaatcagcag
     3541 ttttgaccgt ccgaatattc gctacatgct gatggagaag ttcaaaccgc tcgatcagtt
     3601 gatgcgctac gtgcaggaac agcgcggtaa gtccggcatt atctactgca acagccgggc
     3661 gaaagtagaa gacaccgctg cgcgcctgca aagcaagggt attagcgcgg cggcctatca
     3721 tgccgggctg gaaaataatg tccgcgccga cgtgcaggag aaattccagc gcgatgacct
     3781 gcaaattgtg gtggcgacgg tggcgttcgg catgggcatc aataaaccaa acgttcgctt
     3841 cgtggtccac tttgatattc cgcgcaatat cgaatcctat tatcaggaaa ccggtcgcgc
     3901 cgggcgtgat ggcctgcccg cggaagcgat gctgttttac gatccggccg atatggcgtg
     3961 gctgcgccgt tgtctggaag agaagccgca ggggcagttg caggatatcg aacgtcataa
     4021 actcaatgcg atgggcgctt ttgccgaagc gcaaacttgc cgtcgtctgg tactgctgaa
     4081 ctattttggc gaagggcgtc aggagccgtg cgggaactgc gatatctgcc tcgatccgcc
     4141 gaaacagtac gacggttcaa ccgatgctca gattgccctt tccaccattg gtcgtgtgaa
     4201 tcagcgtttt ggtatgggct atgtggtgga agtgattcgc ggcgctaata accagcgtat
     4261 ccgcgactat ggtcatgaca aactaaaagt ctacggtatg ggccgtgata aaagtcatga
     4321 acattgggtg agcgtgatcc gccagctgat tcacctcggt ctggtgacgc aaaatattgc
     4381 ccagcattct gccctacaac tgacagaggc tgcgcgtccg gtgctgcgcg gcgaatcctc
     4441 tttgcaactt gccgtgccgc gtatcgtggc gctcaaaccg aaagcgatgc agaaatcgtt
     4501 cggcggcaac tatgatcgca aactgttcgc caaattacgc aaactgcgta aatcgatagc
     4561 cgatgaaagt aatgtcccgc cgtacgtggt gtttaacgac gcaaccttga ttgagatggc
     4621 tgaacagatg ccgatcaccg ccagcgaaat gctcagcgtt aacggcgttg ggatgcgcaa
     4681 gctggaacgc tttggcaaac cgtttatggc gctgattcgt gcgcatgttg atggcgatga
     4741 cgaagagtag tcagcagcat aaaaaagtgc cagtatgaag actccgtaaa cgtttccccc
     4801 gcgagtcaaa tgtatgttga tgttatttct caccgtcgcc atggtgcaca ttgtggcgct
     4861 tatgagcccc ggtcccgatt tcttttttgt ctctcagacc gctgtcagtc gttcccgtaa
     4921 agaagcgatg atgggcgtgc tgggcattac ctgcggcgta atggtttggg ctgggattgc
     4981 gctgcttggc ctgcatttga ttatcgaaaa aatggcctgg ctgcatacgc tgattatggt
     5041 gggcggtggc ctgtatctct gctggatggg ttaccagatg ctacgcggtg cactgaaaaa
     5101 agaggcggtt tctgcacctg cgccacaggt cgagctggcg aaaagtgggc gcagtttcct
     5161 gaaaggttta ctgaccaatc tcgctaatcc gaaagcgatt atctactttg gctcggtgtt
     5221 ctcattgttt gtcggtgata acgttggcac taccgcgcgc tggggcattt ttgcgctgat
     5281 cattgtcgaa acgctggcgt ggtttaccgt cgttgccagc ctgtttgccc tgccgcaaat
     5341 gcgccgtggt tatcaacgtc tggcgaagtg gattgatggt tttgccgggg cgttatttgc
     5401 cggatttggc attcatttga ttatttcgcg gtgatgccag acgcgtcttc agagtaagtc
     5461 ggataaggcg tttacgccgc atccgacatt atttttcacg catgcctcgc cgatgctaac
     5521 agcgctccca ccagcataaa caacgagccg aaaatcttat tcagcgcctt catctgcttt
     5581 ggtcctttaa tccatagagc aatccgttga gcaagggtgg cgtaaccgat catcacaata
     5641 atatcgacca caatagtggt gacgccgagc acgatatact gcatcagttg cggctgttgc
     5701 ggcatgatga attgcggaaa tagcgccgcc agaaacacaa tacttttggg attggtgaga
     5761 ttcacaaaaa ctgcgcgctg gaacaaatgt cgacgcgatt gagtagaggc cagcgattta
     5821 aggtcaattg caccagcggc gcgccactgc tggattccca gccaaatcaa gtaagccgcg
     5881 cctgcccact tcaacacttc aaacgcaatc actgagcggg aaaatagcgt ccccaacccc
     5941 acgccaacca gcacaatatg aatcgccagt ccggtctgaa gcccagcaat agacgccacc
     6001 gcgccgcgat aaccgtggtt gagcgaggtg gtcatagtgt tgattgcacc agagcctggc
     6061 gacaggctta aaatgatcga tgtcagcagg taggcaaacc accattctaa ggtcatgatg
     6121 aactcccggt gtgtctattt ttgtgccaca atacgctact gtcgcagcgt tgtgtcaggc
     6181 acgctaaaaa aaacgatttt acgtggttta agaggcagat tacccgatgt ttcagcagca
     6241 aaaagactgg gaaacaagag aaaacgcgtt tgctgctttt accatgggac cgctgactga
     6301 tttctggcgt cagcgtgatg aagcagagtt tactggtgtg gatgacattc cggtgcgctt
     6361 tgtccgtttt cgcgcacagc accatgaccg ggtggtagtc atctgcccgg ggcgtattga
     6421 gagctacgta aaatatgcgg aactggccta tgacctgttc catttggggt ttgatgtctt
     6481 aatcatcgac catcgcgggc agggacgttc cggtcgcctg ttagccgatc cgcatctcgg
     6541 gcatgttaat cgctttaatg attatgttga tgatctggcg gcattctggc agcaggaggt
     6601 tcagcccggt ccgtggcgta aacgctatat actggcacat tcgatgggcg gtgcgatctc
     6661 cacattattt ctgcaacgcc atccaggtgt atgtgacgcc attgcgctaa ctgcgccaat
     6721 gtttgggatc gtgattcgta tgccgtcatt tatggcacgg cagatcctca actgggccga
     6781 agcgcatcca cgtttccgtg atggctatgc aataggcacc gggcgctggc gcgcgttgcc
     6841 gtttgctatc aacgtactga cccacagcag acagcgatat cgacgtaact tacgcttcta
     6901 tgctgatgac ccaacgattc gcgtcggtgg gccgacctac cattgggtac gcgaaagtat
     6961 tctggctggc gaacaggtgt tagccggtgc gggtgatgac gccacgccaa cgcttctctt
     7021 acaagctgaa gaggaacgcg tggtggataa ccgcatgcat gaccgttttt gtgaactccg
     7081 caccgccgcg ggccatcctg tcgaaggagg acggccgttg gtaattaaag gtgcttacca
     7141 tgagatcctt tttgaaaagg acgcaatgcg ctcagtcgcg ctccacgcca tcgttgattt
     7201 tttcaacagg cataactcac ccagcggaaa ccgctctaca gaggtttaaa tttcttatgt
     7261 accaggttgt tgcgtctgat ttagatggca cgttactttc tcccgaccat acgttatccc
     7321 cttacgccaa agaaactctg aagctgctca ccgcgcgcgg catcaacttt gtgtttgcga
     7381 ccggtcgtca ccacgttgat gtggggcaaa ttcgcgataa tctggagatt aagtcttaca
     7441 tgattacctc caatggtgcg cgcgttcacg atctggatgg taatctgatt tttgctcata
     7501 acctggatcg cgacattgcc agcgatctgt ttggcgtagt caacgacaat ccggacatca
     7561 ttactaacgt ttatcgcgac gacgaatggt ttatgaatcg ccatcgcccg gaagagatgc
     7621 gcttttttaa agaagcggtg ttcaaatatg cgctgtatga gcctggatta ctggagccgg
     7681 aaggcgtcag caaagtgttt ttcacctgcg attcccatga acaactgctg ccgctggagc
     7741 aggcgattaa cgctcgctgg ggcgatcgcg tcaacgtcag tttctctacc ttaacctgtc
     7801 tggaagtgat ggcgggcggc gtgtcgaaag gccatgcgct ggaagcagtg gcgaagaaac
     7861 tgggctacag cctgaaggat tgtattgcgt ttggtgacgg gatgaacgac gccgaaatgc
     7921 tgtcgatggc ggggaaaggc tgcattatgg gcagtgcgca ccagcgtctg aaagaccttc
     7981 atcccgagct ggaagtgatt ggtactaatg ccgacgacgc ggtgccgcat tatctgcgta
     8041 aactctattt atcgtaatcg ttctttattt ggtcagttgt caacctgata cttcgctaca
     8101 atggatactc gttaatcaaa gagttttcca ttgtggcgct acttatcatc accacgattc
     8161 tgtgggcctt ctcctttagc ttttatggcg agtaccttgc ggggcacgtc gatagctatt
     8221 ttgcggtgct ggtgcgcgtt ggcctggcgg cactcgtttt tctgccgttt ctgcgtaccc
     8281 gtggcaatag cctgaaaacg gtcggcctgt atatgttggt gggcgcgatg cagctcggcg
     8341 tgatgtatat gctgagtttc cgcgcgtatc tctacctgac ggtctccgaa ctgctgctgt
     8401 tcaccgtact gacgccgctc tacatcacgc tgatttatga catcatgagt aagcgccgtc
     8461 tgcgctgggg ctatgccttt agcgccttgt tggcggtgat tggtgccggg attattcgct
     8521 atgatcaggt caccgaccat ttctggactg gcttgctgct ggtgcaactc tccaatatca
     8581 cttttgccat cggcatggtg ggttacaaac gcctgatgga aactcgcccg atgccacagc
     8641 ataacgcctt tgcgtggttc tatcttggcg cgtttctggt ggcggtaatt gcatggttct
     8701 tgctgggaaa tgcgcagaaa atgccgcaaa ccacgttgca atggggcatt ctggtgtttc
     8761 ttggcgtggt ggcttccggg attggctact ttatgtggaa ctacggcgcg acgcaggtgg
     8821 acgccggaac gctgggcatt atgaataata tgcacgttcc ggcagggctg ctggtaaacc
     8881 tggctatctg gcaccaacag ccgcactggc caacgttcat tacaggcgcg ctggtgatcc
     8941 tggcctcact gtgggtgcat cgtaagtggg tcgctccgcg ctcttcacaa acggcagatg
     9001 atcgcaggcg tgattgcgcg ctgagcgaat aaatgcttct gtaatcggct gacgctgctc
     9061 gccatcgcgc acggcggcgt acagtcggct ccacaagcct tcgcccaggg ttttggtcac
     9121 caccagaccc tggcgctcaa aactctctac tacccaatgc ggtagcgcgg caatacccat
     9181 ccgcgcggca accatctgaa tcaacaataa ggtgttatcg acgcttttca gtgacgggct
     9241 gacgcctgcc ggctgaagaa aatgccgcca gacatccagt cgactacgct gcaccggata
     9301 aatcaacagc gtctcgctgg cgagatcttc cggtgtaatt cgcgttttcg ccgccagtgg
     9361 atggtcaggt gctaacacca gacgcacttc atagtcgaac atcggcgaat aatgcaggcc
     9421 actgcgcggc aggatatcgg aggtcatcac cagatccagc tcgccctgtt gcaacgcggg
     9481 ctgcgggtca aacgtcacgc ccgatttaaa atccatctct acctgcggcc agttcttatg
     9541 gaaattttct aacgcgggcg tcagccactg aatacagcta tggcactcaa tggcaatgcg
     9601 cagacgcgtc tgctgcggtt cattgcaggc ttgcagtgcc tggctaattt gcggcagcac
     9661 ctggtttgcc agttgcaaca ggatttctcc ctgcggtgta aagcgtagcg gctggctctt
     9721 acgcacaaat agccggaagc caaggcgttg ttccagatcg ctaaactggt gagacagggc
     9781 ggattgcgtc tgatgcaacg tcgccgcagc ggctgcgaga gagccgcagt tccgcaacgc
     9841 ttgtagcgtt ttcaggtgtt ttacttcgat catgaaagtc cttcacttcg gcatgaataa
     9901 tttgcgcttg aggaatatac agtaaccgcc aattatggat gtgtaaacat ctggacggct
     9961 aaatactaaa aattccataa ggggcatata atgacaattc ttaatcacac cctcggtttc
    10021 cctcgcgttg gcctgcgtcg cgagctgaaa aaagcgcaag aaagttattg ggcggggaac
    10081 tccacgcgtg aagaactgct gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa
    10141 caaaagcaag cgggtatcga cctgctgccg gtgggcgatt ttgcctggta cgatcatgtt
    10201 ctgaccacca gtctgctgct gggtaatgtt ccgccacgtc atcagaacaa agacggttcg
    10261 gtagatatcg acaccctgtt ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg
    10321 gcggcagcgg aaatgaccaa atggtttaac accaactatc actacatggt gccggaattc
    10381 gttaaaggcc aacagttcaa actgacctgg acgcagctgc tggaggaagt ggacgaggcg
    10441 ctggcgctgg gccacaacgt taagcctgtg ctgctggggc cggttactta cctgtggctg
    10501 gggaaagtga aaggtgaaca gtttgaccgc cttagcctgc tgaacgacat tctgccggtt
    10561 tatcagcaag tgctggcaga actggcgaaa cgcggcatcg agtgggtaca gattgatgaa
    10621 cccgcgctgg tactggaact accacaggcg tggctggacg catacaaacc cgcttacgac
    10681 gcgctccagg gacaggtgaa actgctgctg accacctatt ttgaaggcgt aacgccaaat
    10741 ctcgacacga ttactgcgct gcctgttcag ggtctgcatg ttgacctcgt acatggtaaa
    10801 gatgacgttg ctgaactgca caagcgcctg ccttctgact ggttgctgtc tgcgggtctg
    10861 atcaatggtc gtaacgtctg gcgcgccgat cttaccgaga aatatgcgca aattaaggac
    10921 attgtcggca aacgtgattt gtgggtggca tcttcctgct cgttgctgca cagccccatc
    10981 gacctgagcg tggaaacgcg tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta
    11041 caaaaatgcc atgaactggc actgctgcgc gatgcgctga acagtggtga cacggcagct
    11101 ctggcagagt ggagcgcccc gattcaggca cgtcgtcact ctacccgcgt acataatccg
    11161 gcggtagaaa agcgtctggc ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat
    11221 gaagtgcgtg ccgaagccca gcgtgcgcgt tttaaactgc cagcgtggcc gaccaccacg
    11281 attggttctt tcccgcaaac cacggaaatt cgtaccctgc gtctggattt caaaaagggc
    11341 aatctcgacg ccaataacta ccgcacgggc attgcggaac atatcaggca ggccattgtt
    11401 gaacaggaac gtttgggact ggatgtgctg gtacatggcg aggccgagcg taatgacatg
    11461 gtggaatact ttggcgagca cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag
    11521 agctacggtt cccgctgcgt gaagccaccg attgttattg gtgacgttag tcgcccggca
    11581 ccgattaccg tggagtgggc gaagtatgcg caatcgctga ccgacaaacc ggtgaaaggg
    11641 atgctgacgg ggccggtgac catactctgc tggtcgttcc cgcgtgaaga tgtcagccgt
    11701 gaaaccatcg ccaaacagat tgcgctggcg ctgcgtgatg aggtggccga tctggaagcg
    11761 gcagggattg gtatcatcca gattgacgaa ccggcgctgc gcgaaggttt accactgcgt
    11821 cgtagcgact gggacgccta tctccagtgg ggcgtagagg ccttccgtat caacgccgcc
    11881 gtggcgaaag atgacacaca aatccacact cacatgtgtt attgcgagtt caacgacatc
    11941 atggattcca ttgcggcgct ggatgcagac gtcatcacca tcgaaacctc gcgttccgac
    12001 atggagttgc tggagtcgtt tgaagagttt gattatccga atgaaatcgg tccaggcgtc
    12061 tatgacattc actcgccaaa cgtaccgagc gtggaatgga ttgaagccct gctgaagaaa
    12121 gcggcaaaac gcattccggc agagcgcctg tgggtcaacc cagactgtgg cctgaaaacg
    12181 cgcggctggc cagaaacccg cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg
    12241 cgtctggggt aaatccaaac cgggtggtaa taccacccgg tcttttctca ttacagcgac
    12301 ttcttcccac catactgctt aaaccattcc agcatacgct gccagccatc tttcgcagat
    12361 tcggcatgat agctcgggcg ataatcagcg ttgaatgcat gcccggcatc cgggtagacg
    12421 ataatctctg ctttcgcatt agcagcccgc agcgcctggc gcatcgtttc aacgctctcc
    12481 tgcggaatgc tgttatcctg accgccatat aagccgagaa ccggcgcgtt aagatcggtt
    12541 gcgatatcaa caggttgttt cggtgaattc agcgacttat cgcccgtcag tttgccgtac
    12601 cacgccactg cggcttttag ctgtggatta tgcgcggcat acagccaggt gatacgtcca
    12661 ccccagcaga atccggtgat cattaaacga tgaacatcgc cgccgttgcg ggaagcccaa
    12721 ctggcgacat gatcgagatc ggccagcacc tgcgagtcag gcacttttgc taccagaccg
    12781 ctaagcaacg tggggatatc ggcaaaatca ttcggatcgc cttcgcggaa gtaaagttca
    12841 ggtgcgatag ccagataccc ctccagcgcc agacggcgac aaatgtcgcg gatatgttca
    12901 tgcacgccaa aaatttcctg cactacaatg accactggca gtgggccatc gctttgcttt
    12961 ggtctggcat ggtaagcagg catgttatcc ccttgtgaag ggatagaggt gaagcccgcc
    13021 acaatcgcgt cgtccggggt ctgaacgatg gtcgaagcga gaggcgatgc agcaggtgca
    13081 aatccagatt gttgtgttgt tgccatggta ttctccgtac ccttataaaa atgttgcgca
    13141 atgttaacta tagtcagcat gcaacaaatc acattgcctg aatcggctca tcttttatgc
    13201 agtcctgcag aatgaagagt gatttatgtg atttgcatca cttttggtgg gtaaatttat
    13261 gcaacgcatt tgcgtcatgg tgatgagtat cacgaaaaaa tgttaaaccc ttcggtaaag
    13321 tgtctttttg cttcttctga ctaaaccgat tcacagagga gttgtatatg tccaagtctg
    13381 atgtttttca tctcggcctc actaaaaacg atttacaagg ggctacgctt gccatcgtcc
    13441 ctggcgaccc ggatcgtgtg gaaaagatcg ccgcgctgat ggataagccg gttaagctgg
    13501 catctcaccg cgaattcacc acctggcgtg cagagctgga tggtaaacct gttatcgtct
    13561 gctctaccgg tatcggcggc ccgtctacct ctattgctgt tgaagagctg gcacagctgg
    13621 gcattcgcac cttcctgcgt atcggtacaa cgggcgctat tcagccgcat attaatgtgg
    13681 gtgatgtcct ggttaccacg gcgtctgtcc gtctggatgg cgcgagcctg cacttcgcac
    13741 cgctggaatt cccggctgtc gctgatttcg aatgtacgac tgcgctggtt gaagctgcga
    13801 aatccattgg cgcgacaact cacgttggcg tgacagcttc ttctgatacc ttctacccag
    13861 gtcaggaacg ttacgatact tactctggtc gcgtagttcg tcactttaaa ggttctatgg
    13921 aagagtggca ggcgatgggc gtaatgaact atgaaatgga atctgcaacc ctgctgacca
    13981 tgtgtgcaag tcagggcctg cgtgccggta tggtagcggg tgttatcgtt aaccgcaccc
    14041 agcaagagat cccgaatgct gagacgatga aacaaaccga aagccatgcg gtgaaaatcg
    14101 tggtggaagc ggcgcgtcgt ctgctgtaat tctcttctcc tgtctgaagg ccgacgcgtt
    14161 cggccttttg tatttttgcg tagcgcctcg caggaaatgc ctttccaact ggacgtttgt
    14221 acagcacaat tctattttgt gcgggtaagt tgttgcgtca ggaggcgttg tggatttctc
    14281 aatcatggtt tacgcagtta ttgcgttggt gggtgtggca attggctggc tgtttgccag
    14341 ttatcaacat gcgcagcaaa aagccgagca attagctgaa cgtgaagaga tggtcgcgga
    14401 gttaagcgcg gcaaaacaac aaattaccca aagcgagcac tggcgtgcag agtgcgagtt
    14461 actcaataac gaagtgcgca gcctgcaaag tattaacacc tctctggagg ccgatctgcg
    14521 tgaagtaacc acgcggatgg aagccgcaca gcaacatgct gacgataaaa ttcgccagat
    14581 gattaacagc gagcagcgcc tcagtgagca gtttgaaaac ctcgccaacc gtatttttga
    14641 gcacagcaat cgccgggttg atgagcaaaa ccgtcagagt ctgaacagcc tgttgtcgcc
    14701 gctacgtgaa caactggacg gtttccgccg tcaggttcag gacagcttcg gtaaagaagc
    14761 acaagaacgc cataccctga cccacgaaat tcgcaatctc cagcaactca acgcgcaaat
    14821 ggcccaggaa gcgatcaacc tgacgcgcgc gctgaaaggc gacaataaaa cccagggcaa
    14881 ctggggcgag gtagtattga cgcgggtgct ggaggcttcc ggtctgcgtg aagggtatga
    14941 atatgaaacc caggtcagca tcgaaaatga cgcccgctcg cggatgcagc cggatgtcat
    15001 cgtgcggctg ccgcagggaa aagatgtggt gatcgacgcc aaaatgacgc tggtcgccta
    15061 tgaacgctat tttaacgccg aagacgacta cacccgcgaa agcgcgctac aggaacatat
    15121 cgcgtcggtg cgtaaccata tccgtttgct gggacgcaaa gattatcaac agctgccggg
    15181 gctgcgaact ctggattacg tgctgatgtt tattcccgtt gaacccgctt tttcactggc
    15241 gcttgaccgc cagccggagc tgatcaccga agcgttgaaa aacaatatca tgctggttag
    15301 cccgactacg ctgctggtgg cgttgcgtac tattgccaac ctgtggcgtt atgagcatca
    15361 aagccgcaac gcccagcaaa tcgccgatcg tgccagcaag ctgtacgaca agatgcgttt
    15421 gttcatcgat gacatgtccg cgattggtca aagtctcgac aaagcgcagg ataactaccg
    15481 ccaggcaatg aaaaaactct cttcaggtcg cggaaatgtg ctggcgcagg cagaagcgtt
    15541 tcgcggttta ggagtagaaa ttaaacgcga gattaatccg gatttggctg aacaggcggt
    15601 gagccaggat gaagagtatc gacttcggtc ggttccggag cagccgaatg atgaagctta
    15661 tcaacgcgat gatgaatata atcagcagtc gcgctagccc attaggggta gttaagccgg
    15721 gtagaaatct agggcatcga cgcccaatct gttacacttc tggaacaatt ttttgatgag
    15781 caggcattga gatggtggat aagtcacaag aaacgacgca ctttggtttt cagaccgtcg
    15841 cgaaggaaca aaaagcggat atggtcgccc acgttttcca ttccgtggca tcaaaatacg
    15901 atgtcatgaa tgatttgatg tcatttggta ttcatcgttt gtggaagcga ttcacgattg
    15961 attgcagcgg cgtacgccgt gggcagaccg tgctggatct ggctggtggc accggcgacc
    16021 tgacagcgaa attctcccgc ctggtcggag aaactggcaa agtggtcctt gctgatatca
    16081 atgaatccat gctcaaaatg ggacgcgaga agctgcgtaa tatcggtgtg attggcaacg
    16141 ttgagtatgt tcaggcgaac gctgaggcgc tgccgttccc ggataacacc tttgattgca
    16201 tcaccatttc gtttggtctg cgtaacgtca ccgacaaaga taaagcactg cgttcaatgt
    16261 atcgcgtgct gaaacccggc ggccgcctgc tggtgcttga gttctcgaag ccaattatcg
    16321 agccgctgag caaagcctat gatgcatact ccttccatgt gctgccgcgt attggctcac
    16381 tggtcgcgaa cgacgccgac agctaccgtt atctggcaga atccatccgt atgcatcccg
    16441 atcaggatac cctgaaagcc atgatgcagg atgccggatt cgaaagtgtc gactactaca
    16501 atctgacggc gggggttgtg gcgctgcatc gtggttataa gttctgacag gagaccggaa
    16561 atgcctttta aacctttagt gacggcagga attgaaagtc tgctcaacac cttcctgtat
    16621 cgctcacccg cgctgaaaac agcccgctcg cgtctgctgg gtaaagtatt gcgcgtggag
    16681 gtaaaaggct tttcgacgtc attgattctg gtgttcagcg aacgccaggt tgatgtactg
    16741 ggcgaatggg caggcgatgc tgactgcacc gttatcgcct acgccagtgt gttgccgaaa
    16801 cttcgcgatc gccagcagct taccgcactg attcgcagtg gtgagctgga agtgcagggc
    16861 gatattcagg tggtgcaaaa cttcgttgcg ctggcagatc tggcagagtt cgaccctgcg
    16921 gaactgctgg ccccttatac cggtgatatc gccgctgaag gaatcagcaa agccatgcgc
    16981 ggaggcgcaa agttcctgca tcacggcatt aagcgccagc aacgttatgt ggcggaagcc
    17041 attactgaag agtggcgtat ggcacccggt ccgcttgaag tggcctggtt tgcggaagag
    17101 acggctgccg tcgagcgtgc tgttgatgcc ctgaccaaac ggctggaaaa actggaggct
    17161 aaatgacgcc aggtgaagta cggcgcctat atttcatcat tcgcactttt ttaagctacg
    17221 gacttgatga actgatcccc aaaatgcgta tcaccctgcc gctacggcta tggcgatact
    17281 cattattctg gatgccaaat cggcataaag acaaactttt aggtgagcga ctacgactgg
    17341 ccctgcaaga actggggccg gtttggatca agttcgggca aatgttatca acccgccgcg
    17401 atctttttcc accgcatatt gccgatcagc tggcgttatt gcaggacaaa gttgctccgt
    17461 ttgatggcaa gctggcgaag cagcagattg aagctgcaat gggcggcttg ccggtagaag
    17521 cgtggtttga cgattttgaa atcaagccgc tggcttctgc ttctatcgcc caggttcata
    17581 ccgcgcgatt gaaatcgaat ggtaaagagg tggtgattaa agtcatccgc ccggatattt
    17641 tgccggtcat taaagcggat ctgaaactta tctaccgtct ggctcgctgg gtgccgcgtt
    17701 tgctgccgga tggtcgccgt ctgcgcccaa ccgaagtggt gcgcgagtac gaaaagacct
    17761 tgattgatga actgaatttg ctgcgggaat ctgccaacgc cattcagctt cggcgcaatt
    17821 ttgaagacag cccgatgctc tacatcccgg aagtttaccc tgactattgt agtgaaggga
    17881 tgatggtgat ggagcgcatt tacggcattc cggtgtctga tgttgcggcg ctggagaaaa
    17941 acggcactaa catgaaattg ctggcggaac gcggcgtgca ggtgttcttc actcaggtct
    18001 ttcgcgacag ctttttccat gccgatatgc accctggcaa catcttcgta agctatgaac
    18061 acccggaaaa cccgaaatat atcggcattg attgcgggat tgttggctcg ctaaacaaag
    18121 aagataaacg ctatctggca gaaaacttta tcgccttctt taatcgcgac tatcgcaaag
    18181 tggcagagct acacgtcgat tcaggctggg tgccaccaga taccaacgtt gaagagttcg
    18241 aatttgccat tcgtacggtc tgtgaaccta tctttgagaa accgctggcc gaaatttcgt
    18301 ttggacatgt actgttaaat ctgtttaata cggcgcgtcg cttcaatatg gaagtgcagc
    18361 cgcaactggt gttactccag aaaaccctgc tctatgtcga aggggtagga cgccagcttt
    18421 atccgcagct cgatttatgg aaaacagcga agcctttcct ggagtcgtgg attaaagatc
    18481 aggtcggtat tcctgcgctg gtgagagcat ttaaagaaaa agcgccgttc tgggtcgaaa
    18541 aaatgccaga actgcctgaa ttggtttacg acagtttgcg ccagggcaag tatttacagc
    18601 acagtgttga taagattgcc cgcgagcttc agtcaaatca tgtacgtcag ggacaatcgc
    18661 gttattttct cggaattggc gctacgttag tattaagtgg cacattcttg ttggtcagcc
    18721 gacctgagtg ggggctgatg cccggctggt taatggcagg tggtctgatc gcctggtttg
    18781 tcggttggcg caaaacacgc tgattttttc atcgctcaag gcgggccgtg taacgtataa
    18841 tgcggctttg tttaatcatc atctaccaca gaggaacatg tatgggtggt atcagtattt
    18901 ggcagttatt gattattgcc gtcatcgttg tactgctttt tggcaccaaa aagctcggct
    18961 ccatcggttc cgatcttggt gcgtcgatca aaggctttaa aaaagcaatg agcgatgatg
    19021 aaccaaagca ggataaaacc agtcaggatg ctgattttac tgcgaaaact atcgccgata
    19081 agcaggcgga tacgaatcag gaacaggcta aaacagaaga cgcgaagcgc cacgataaag
    19141 agcaggtgta atccgtgttt gatatcggtt ttagcgaact gctattggtg ttcatcatcg
    19201 gcctcgtcgt tctggggccg caacgactgc ctgtggcggt aaaaacggta gcgggctgga
    19261 ttcgcgcgtt gcgttcactg gcgacaacgg tgcagaacga actgacccag gagttaaaac
    19321 tccaggagtt tcaggacagt ctgaaaaagg ttgaaaaggc gagcctcact aacctgacgc
    19381 ccgaactgaa agcgtcgatg gatgaactac gccaggccgc ggagtcgatg aagcgttcct
    19441 acgttgcaaa cgatcctgaa aaggcgagcg atgaagcgca caccatccat aacccggtgg
    19501 tgaaagataa tgaagctgcg catgagggcg taacgcctgc cgctgcacaa acgcaggcca
    19561 gttcgccgga acagaagcca gaaaccacgc cagagccggt ggtaaaacct gctgcggacg
    19621 ctgaaccgaa aaccgctgca ccttcccctt cgtcgagtga taaaccgtaa acatgtctgt
    19681 agaagatact caaccgctta tcacgcatct gattgagctg cgtaagcgtc tgctgaactg
    19741 cattatcgcg gtgatcgtga tattcctgtg tctggtctat ttcgccaatg atatctatca
    19801 cctggtatcc gcgccattga tcaagcagtt gccgcaaggt tcaacgatga tcgccaccga
    19861 cgtggcctcg ccgttcttta cgccgatcaa gctgaccttt atggtgtcgc tgattctgtc
    19921 agcgccggtg attctctatc aggtgtgggc atttatcgcc ccagcgctgt ataagcatga
    19981 acgtcgcctg gtggtgccgc tgctggtttc cagctctctg ctgttttata tcggtatggc
    20041 attcgcctac tttgtggtct ttccgctggc atttggcttc cttgccaata ccgcgccgga
    20101 aggggtgcag gtatccaccg acatcgccag ctatttaagc ttcgttatgg cgctgtttat
    20161 ggcgtttggt gtctcctttg aagtgccggt ggcaattgtg ctgctgtgct ggatggggat
    20221 tacctcgcca gaagacttac gcaaaaaacg cccgtatgtg ctggttggtg cattcgttgt
    20281 cgggatgttg ctgacgccgc cggatgtctt ctcgcaaacg ctattggcga tcccgatgta
    20341 ctgtctgttt gaaatcggtg tcttcttctc acgcttttac gttggtaaag ggcgaaatcg
    20401 ggaagaggaa aacgacgctg aagcagaaag cgaaaaaact gaagaataaa ttcaaccgcc
    20461 cgtcagggcg gttgtcatat ggagtacagg atgtttgata tcggcgttaa tttgaccagt
    20521 tcgcaatttg cgaaagaccg tgatgatgtt gtagcgcgcg cttttgacgc gggagttaat
    20581 gggctactca tcaccggcac taacctgcgt gaaagccagc aggcgcaaaa gctggcgcgt
    20641 cagtattcgt cctgttggtc aacggcgggc gtacatcctc acgacagcag ccagtggcaa
    20701 gctgcgactg aagaagcgat tattgagctg gccgcgcagc cagaagtggt ggcgattggt
    20761 gaatgtggtc tcgactttaa ccgcaacttt tcgacgccgg aagagcagga acgcgctttt
    20821 gttgctcagc tacgcattgc cgcagaatta aacatgccgg tatttatgca ctgtcgcgat
    20881 gcccacgagc ggtttatgac attgctggag ccgtggctgg ataaactgcc tggtgcggtt
    20941 cttcattgct ttaccggcac acgcgaagag atgcaggcgt gcgtggcgcg tggaatttat
    21001 atcggcatta ccggttgggt ttgcgatgaa cgacgcgggc tggagctgcg ggaattgttg
    21061 ccgttgattc cggcggagaa attgctgatc gaaactgatg cgccgtatct gctcccgcgc
    21121 gatctcacgc caaagccatc atcccggcgc aacgagccag cccatctgcc ccatattttg
    21181 caacgtattg cgcactggcg tggagaagat gccgcatggc tggctgccac cacggatgct
    21241 aatgtcaaaa cactgtttgg gattgcgttt tagagtttgc ggaactcggt gttcttcaca
    21301 ctgtgcttaa tctctttatt aataagatta agcaatagca tggagcgagc ctcaccatcg
    21361 ggttcggtga aaatggcctg aaagccttcg aacgcgcctt cggtaataat caccttatca
    21421 cccggataag gggttgccgg atcgacaatg tctttcggtt tatataccga tagctgatga
    21481 atcaccgccg atgggactat cgctggcgac gcgccaaagc gcacgaagtg gctgacaccg
    21541 cgggtcgcgt tgatagtcgt ggtatgaatc acttctggat caaattcgac aaacaggtag
    21601 ttgggaaaca atggctcact gactgcagta cgttttccac gcacgatttt ttccagggtg
    21661 atcatcggtg ccaggcaatt cacagcctgt ctttcgaggt gttcctgggc acgttgaagt
    21721 tgcccgcgct tgcagtacag taaataccag gattgcataa tgactcttat ccgtttaatc
    21781 ggggcgcaag gatagcaaaa gctttacgct aagttaatta tattccccgg tttgcgttat
    21841 accgtcagag ttcacgctaa tttaacaaat ttacagcatc gcaaagatga acgccgtata
    21901 atgggcgtag attaagaggc tacaatggac gccatgaaat ataacgattt acgcgacttc
    21961 ttgacgctgc ttgaacagca gggtgagcta aaacgtatca cgctcccggt ggatccgcat
    22021 ctggaaatca ctgaaattgc tgaccgcact ttgcgtgccg gtgggcctgc gctgttgttc
    22081 gaaaacccta aaggctactc aatgccggtg ctgtgcaacc tgttcggtac gccaaagcgc
    22141 gtggcgatgg gcatggggca ggaagatgtt tcggcgctgc gtgaagttgg taaattattg
    22201 gcgtttctga aagagccgga gccgccaaaa ggtttccgcg acctgtttga taaactgccg
    22261 cagtttaagc aagtattgaa catgccgaca aagcggctgc gtggtgcgcc ctgccaacaa
    22321 aaaatcgtct ctggcgatga cgtcgatctc aatcgcattc ccattatgac ctgctggccg
    22381 gaagatgccg cgccgctgat tacctggggg ctgacagtga cgcgcggccc gcataaagag
    22441 cggcagaatc tgggcattta tcgccagcag ctgattggta aaaacaaact gattatgcgc
    22501 tggctgtcgc atcgcggcgg cgtgctggat tatcaggagt ggtgtgcggc gcatccgggc
    22561 gaacgtttcc cggtttctgt ggcgctgggt gccgatcccg ccacgattct cggtgcagtc
    22621 acccccgttc cggatacact ttcagagtat gcgtttgccg gattgctgcg cggtaccaag
    22681 accgaagtgg tgaagtgtat ctccaatgac cttgaagtgc ccgccagtgc ggagattgtg
    22741 ctggaagggt atatcgaaca aggtgaaacg gcgccggaag ggccgtatgg cgaccacacc
    22801 ggttactata acgaagtcga tagtttcccg gtatttaccg tgacgcatat tacccagcgt
    22861 gaagatgcga tttaccattc cacctatacc gggcgtccgc cagatgagcc cgcggtactg
    22921 ggcgtggcgt tgaacgaagt gtttgtaccg attctgcaaa aacagttccc ggaaattgtc
    22981 gatttttacc tgccgccgga aggctgttct tatcgtctgg cggtagtgac gatcaaaaaa
    23041 cagtacgccg gacacgcgaa gcgcgtcatg atgggcgtct ggtcgttctt acgccagttt
    23101 atgtacacta aatttgtgat cgtttgcgat gatgacgtca acgcacgcga ctggaacgat
    23161 gtgatttggg cgattaccac ccgtatggac ccggcgcggg atactgttct ggtagaaaat
    23221 acgcctattg attatctgga ttttgcctcg cctgtctccg ggctgggttc aaaaatgggg
    23281 ctggatgcca cgaataaatg gccgggggaa acccagcgtg aatggggacg tcccatcaaa
    23341 aaagatccag atgttgtcgc gcatattgac gccatctggg atgaactggc tatttttaac
    23401 aacggtaaaa gcgcctgatg cgcgtttgtt ttgccctatt tatcgatccg acagagaaag
    23461 cgcatgacaa ccttaagctg taaagtgacc tcggtagaag ctatcacgga taccgtatat
    23521 cgtgtccgca tcgtgccaga cgcggccttt tcttttcgtg ctggtcagta tttgatggta
    23581 gtgatggatg agcgcgacaa acgtccgttc tcaatggcct cgacgccgga tgaaaaaggg
    23641 tttatcgagc tgcatattgg cgcttctgaa atcaaccttt acgcgaaagc agtcatggac
    23701 cgcatcctca aagatcatca aatcgtggtc gatattcccc acggagaagc gtggctgcgc
    23761 gatgatgaag agcgtccgat gattttgatt gcgggcggca ccgggttctc ttatgcccgc
    23821 tcgattttgc tgacagcgtt ggcgcgtaac ccaaaccgtg atatcaccat ttactggggc
    23881 gggcgtgaag agcagcatct gtatgatctc tgcgagcttg aggcgctttc gttaaagcac
    23941 cctggtctgc aagtggtgcc ggtggttgaa caaccggaag caggctggcg tgggcgtact
    24001 ggtaccgtgt taacggcggt attgcaggat catggtacgc tggcagagca tgatatctat
    24061 attgccggac gttttgagat ggcgaaaatt gcccgtgacc tgttttgcag tgagcgtaat
    24121 gcgcgggaag atcgcctgtt tggcgatgcg tttgcattta tctgagatat aaaaaaaccc
    24181 gcccctgaca ggcgggaaga acggcaacta aaactttatt cagtggcatt tagatctatg
    24241 tcgcatccgg caatcggtgc acgatgcctg atgcgacgct ggcgcgtctt atcaggccta
    24301 cattggtgca ggatcggtag accggataag gcgttcacgc cgcatccggc aagtggttaa
    24361 acccgctcaa acaccgtcgc aataccctga cccagaccga tacacatcgt cgccagacca
    24421 aactgaacgt ctttgcgttc catcagattc agcagcgtgg tgctgatacg cgcaccggaa
    24481 cagcccagcg gatgacccag cgcgatcgca ccaccgttga ggttgatctt ctcgtcaatc
    24541 tgctcaatca gtcccagatc tttaatacat ggcaggatct gcgcggcaaa ggcttcgttc
    24601 atctcaaaca cgccgatatc gctggcagaa agccccgctt ttttcagcgc cagcttcgag
    24661 gccggaaccg ggccgtaacc cataatcgat gggtcacaac caacgaccgc catcgaacgc
    24721 acacgagcgc gcggcttaag acctaattca tgggcgcggc tttcactcat caccagcatg
    24781 gcagctgcgc catcggaaag tgcagaagat gtgcccgccg ttaccgtacc gtttactgga
    24841 tcaaacgccg gacgcagcgt ggcgagggct tccacggtgg tttccgggcg aatcacttcg
    24901 tcgtaattaa actgcttcag gacgccgtcg gcatcgtgac caccagttgg gatgatttca
    24961 tttttaaatg cggccgactg cgtggcggcc caggcgcggg cgtgtgaccg cgcggcaaag
    25021 gcatcctgca tttcacggct gataccgtgc atacgcgcca gcatttctgc tgttaagccc
    25081 atcatgcccg ccgctttggc gacattgcgg ctcaggccgg ggtgaaaatc gacgccgtga
    25141 ctcatcggca catggcccat atgctccacg ccgccaacca gacatgcctg cgcatcgccg
    25201 gtcataatca tccgtgctgc gtcatgcagt gcctgcatgg atgaaccaca caagcgattg
    25261 acggtaaccg ccgggacaga gtgtggtact tctgccagca gcgccgcgtt acgggcgatg
    25321 ttaaaaccct gctccagcgt ctgctgcaca caaccccagt aaatatcgtc gagggccgcc
    25381 gcttccagcg ccgggttacg cgccagcagg ctacgcatta aatgagcgga gagatcttct
    25441 gcacgcacgt tacgaaaagc accgcccttc gaacggccca tcggggtgcg aattgcatcg
    25501 acaatgacaa cctgttccat tgtgactcct taagccgttt tcaggtcgcc aaccggacgg
    25561 gctggctcaa ccggaggata gtacggttcg ttatgacgcg ctttattacg cagaccttcc
    25621 ggcacttcat acagcgggcc gaggtgctga tattgctgtg ccatatcgag gtattttgcg
    25681 ctaccgaggg tgtccagcca gcggaacgcg ccgccgtgga acggagggaa gcccaggccg
    25741 tagaccagcg ccatatccgc ttccgccgga gtggcgataa tgccttcctc cagacagcgc
    25801 accacttcgt tgaccatcgg gatcatcatg cgggcgataa tctcctcttc gctgaaatcg
    25861 cgcttcggct ggctcacttc tgccagcagg tcttcaacgg cggcgtcttc ttctttcttc
    25921 ggcttacctt tgctgtcttc tttataacgc cagaaaccga ggccgttctt ctgaccaaag
    25981 cggttggcat caaacagcgc gtcgatggca tcgcggtaat ctttctgcat cctctgcggg
    26041 aagcctgctg ccatgacagc ctgggcatga tgcgcggtgt caatgcccac gacatccagc
    26101 agatatgccg gacccatcgg ccagccaaac tgtttttcca tcactttgtc gatcttgcgg
    26161 aaatccgcgc cgtcgcgcag cagctggctg aaaccggcga aatacgggaa cagcacgcgg
    26221 ttaacaaaga agccggggca gtcgttaacc acaatcggcg tcttgcccat cttgctcgcc
    26281 caggcgacaa ctttcgcgat ggtttcgtcg gagcttttct cgccgcgaat aatttctacc
    26341 aacggcattc ggtggaccgg gttaaagaag tgcatcccgc agaagttttc cgggcgtttc
    26401 agcgcgttgg ccagttcgct gataggaatg gttgaagtgt tagacgccag cacggtatcc
    26461 tggcgtactt tttgttcggt ttctgccagt acggcttttt tcactttcgg gttttcaaca
    26521 accgcttcta ccacaatatc cacgcggtca aatccggcgt agtcgagcgt tgggtggatt
    26581 gtggagatca cgccagccag tttcagacca tcgatcttgc cgcgctcaag ctgcttgttc
    26641 agcagtttcg cggcttcggt catgccgagg gttaacgact tgtcgttgat atctttcatg
    26701 acaaccggca cgcctttcca cgcagactgg taagcgatgc cgccgcccat aatgcctgca
    26761 cccagcaccg cggcctgttt cggggtttca acgtctttgg tgagtttctt cgctttgcct
    26821 tttacatatt gatcgttaag gaaaatgccg accagtgcgc gggcttcgtt ggtatgcgcc
    26881 agcgggacaa aacttttgtt ttccaggttt aaggcttctt cacgaccaaa acgggccgca
    26941 gcttcaatgg tttttactgc ggtgatgggg gccggataat gtttccccgc tgtttgtgcg
    27001 accatccctt tagcgatggt gaagctcatg gtggcttcaa tcttgctcag ttttagtggt
    27061 tccagcttcg gctgacgttt tgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa
    27121 accgcctttg cgccttcaac cagtttttct gctttgacta cgccatccac cagaccgatt
    27181 ttcagcgcct gatccgcgcc gacatcttta ccggcggcaa tgatttccag cgcactgtct
    27241 gcgcccagca tacgtggcat acgtacagaa ccgccaaagc ccggcatgat gcccagtttg
    27301 gtttccggca gaccgatgcg cagatccggc gtcgccagac gataatcggt cgccagcacg
    27361 cattcgcagc caccgcccag cgcatagccg ttgacggcag caatggtcgg caccggcaga
    27421 tcttccaggc gattaaacac gctattggca aagtgcagcc actgacttaa ctgttcttca
    27481 ggaacgagga acagggacaa aaattcggtg atatcagcac cgacgataaa ggctgctttg
    27541 ttcgaacgca gcagcagccc ttttagatct gattgctgtt ccagcacgcc gatggcctcg
    27601 ccgaggctgg cgacggtcgc agtgtcgagt ttattaactg aacctggggc atcaaatacc
    27661 agttcggcaa tgccatcttc cagccagtca aggtacaggg tgtcgccttt gtaaagcatg
    27721 tcagtctcct gaatccgcaa ggtgatctgg tcgtaccaga tgcatcgaag tgtgtatttt
    27781 gtgttaaaaa tatgcaaata aaagattaaa gaaatgccga tctgatcacg ctcggcagaa
    27841 atcacgctct ggatgaacga tgtgctaaga tgcggagact taaggtcaaa aaaacagaag
    27901 ggtaaaaaat ggaatcactg gcctcactct ataaaaatca tatagctacc ttacaggaac
    27961 ggactcgcga tgcgctggcg cgcttcaagc tggatgcgtt acttattcac tccggcgaac
    28021 tgttcaacgt ttttctcgac gatcatccct atccgtttaa agtgaacccg caattcaaag
    28081 cgtgggtgcc ggtaactcag gtgccaaact gctggctgct ggtggatggc gtgaacaagc
    28141 cgaaactgtg gttctatctg ccggttgatt actggcacaa cgtcgaaccg ctgccgacct
    28201 ctttctggac tgaagacgtg gaagtgatcg cgctgccgaa agccgatggc attggtagcc
    28261 tgctgcctgc tgcgcgcggg aatatcggtt atatcggtcc ggtgccggaa cgtgcgctgc
    28321 aactgggtat tgaggccagc aatatcaacc cgaaaggggt gatcgactac ctgcattact
    28381 accgctcctt caaaaccgag tacgaactgg cctgtatgcg tgaagcgcag aaaatggcgg
    28441 tcaacggtca tcgcgcggca gaagaagcgt tccgttctgg catgagcgag ttcgatatca
    28501 acattgccta tctgaccgcg accggtcatc gtgataccga cgtaccttac agcaacattg
    28561 tggcgctcaa cgaacacgct gcggtgttgc attacaccaa actggaccac caggcaccgg
    28621 aagagatgcg cagcttcctg ctggatgccg gggccgaata taacggctat gccgctgacc
    28681 tgacccgtac ctggtcggca aaaagtgaca acgattacgc acagctggtg aaagacgtaa
    28741 atgatgaaca actggcgctg atcgcgacca tgaaagctgg cgttagctat gtggattacc
    28801 acatccagtt ccatcagcgc atcgccaaac tgctgcgtaa acatcaaatc atcaccgata
    28861 tgagtgaaga agcgatggtc gaaaacgatc tcaccggacc gtttatgccg cacggtatcg
    28921 gccatccgct gggcctgcag gtgcatgacg tcgccggttt tatgcaggat gatagcggta
    28981 cgcacctcgc ggcaccggca aaatatccgt acctgcgctg cacccgtatt ctccagccgg
    29041 gcatggtgtt aaccatcgaa ccgggtatct acttcattga atcgctactg gcaccgtggc
    29101 gtgaagggca gttcagcaag cacttcaact ggcagaaaat tgaagcactg aaaccgttcg
    29161 gcggcattcg tatcgaagac aacgtggtga tccacgaaaa taacgtggaa aacatgaccc
    29221 gggatctgaa actggcgtga tggaaagctg gttaattcct gcggcaccgg tcacggtcgt
    29281 tgaagagatc aaaaagagcc gcttcataac gatgttggcg cataccgatg gcgttgaggc
    29341 ggcgaaagcg tttgttgaat cggtgcgggc agaacacccc gatgcccgcc accattgcgt
    29401 ggcgtgggtg gcgggtgcgc cggatgattc acaacagctg ggtttctctg acgacgggga
    29461 gccggcggga acggcaggta aaccgatgct cgcccagcta atgggcagcg gcgttgggga
    29521 aattaccgct gtggtggtgc gctactacgg cggcatattg cttggcaccg gtgggttagt
    29581 gaaagcgtat ggcggcggcg tgaatcaggc gctgcgccag ctaacgaccc aacgcaagac
    29641 gccattaacc gaatatactt tgcaatgtga atatcatcag ttaaccggca ttgaagcgtt
    29701 gctggggcag tgtgacggca aaattatcaa cagtgattat caggcattcg ttctgctgcg
    29761 ggtggcgctt ccggcggcga aagtggctga attttccgca aagctggcgg attttagccg
    29821 tggttcattg caattgttag cgattgaaga ataatcccca cttcgttttg cagaactaag
    29881 gaagcggcag agatgcattt tcgcgccatt acccgaatcg ttggactact ggtcatctta
    29941 ttttcaggga ccatgattat ccctgggctg gtagcactca tctaccggga tggagcgggc
    30001 cgcgctttta cccagacctt ttttgtcgcc ctcgccattg gctctatgct gtggtggccg
    30061 aaccgcaaag agaaaggcga acttaaatcc cgtgaggggt ttctgatagt ggtgctgttc
    30121 tggaccgtgc tgggtagcgt cggtgcgctc ccttttatct tctcggaaag cccgaacctc
    30181 acgattaccg atgcgttttt tgaatctttc tctggcctga ccaccaccgg ggccactacg
    30241 ctggtggggc tggattcgct ccctcacgcc atcctctttt atcgccagat gctgcaatgg
    30301 tttggcggga tggggatcat cgtgttagcg gttgcgatac tgcctatcct cggcgtgggt
    30361 gggatgcagc tctatcgcgc agaaatgccc ggcccgctga aagataacaa aatgcgcccg
    30421 cgaattgcgg aaacggcgaa aaccctgtgg ttgatttatg tcttgctgac cgtcgcctgt
    30481 gcgctggcgt tgtggtttgc cggaatggat gcctttgatg ccatcggcca tagctttgcg
    30541 actatcgcta ttggcggctt ctcgacacat gatgccagta tcggttattt cgatagcccg
    30601 actattaaca ctatcattgc tatcttcctg ctgatctccg gctgtaacta cggtctgcac
    30661 ttttcactgt taagtgggcg tagtctgaag gtttattggc gcgatccgga atttcgcatg
    30721 tttatcggcg tacagtttac gctggtggtt atttgtaccc tcgtactgtg gtttcataat
    30781 gtctacagtt cggcgctgat gacaattaac caggcgtttt tcctggtggt gtcgatggcg
    30841 acaaccgccg ggtttacgac tgacagcatt gcccgctggc cgctcttttt gccggtactg
    30901 cttttatgtt cggcttttat cggcggttgt gccgggtcaa cgggcggtgg cctgaaagtg
    30961 atccgcatcc tgctgctgtt taagcagggg aaccgtgagc tgaaacgact ggtgcatccg
    31021 aacgccgtct atagcattaa gctggggaat cgcgcactgc cggaacgtat cctcgaagcc
    31081 gtttggggat ttttctccgc ctatgcattg gtgtttattg tcagtatgct ggcgattatc
    31141 gccacgggcg tggatgactt ttctgccttt gcctcagttg ttgcgacatt gaataacctg
    31201 gggccagggc ttggcgtggt tgctgataac tttaccagta tgaacccggt ggctaaatgg
    31261 atcctgattg ccaacatgct gtttggtcgt ctcgaggtct ttacattgct ggtgctcttt
    31321 accccgactt tctggcgtga atgatggagt aatacgtgaa aacattaatt cttttctcaa
    31381 caagggacgg acaaacgcgc gagattgcct cctacctggc ttcggaactg aaagaactgg
    31441 ggatccagac ggatgtcgcc aatgtgcacc gcattgaaga accacaatgg gaaaattatg
    31501 accgtgtggt cattggtgct tctattcgct atggtcacta ccactctgcg ttccaggaat
    31561 ttgtcaaaaa acatgcgacg cgactgaatt cgatgccgag cgccttttat tccgtaaacc
    31621 tggtggcgcg caaaccggag aagcgtactc cacagaccaa cagctacgcg cggaagtttc
    31681 tgatgaactc gcaatggcgt cccgatcgct gcgcggtcat tgccggggcg ctgcgttacc
    31741 cacgttatcg ctggtacgac cgttttatga tcaagctgat tatgaagatg tcaggcggtg
    31801 aaacggatac gcgcaaagaa gttgtctata ccgattggga gcaggtggcg aatttcgccc
    31861 gagaaatcgc ccatttaacc gacaaaccga cgctgaaata agcacaaaga a
//
LOCUS       DAZGOB010000048        30997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGOB010000048 DAZGOB010000000
VERSION     DAZGOB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.048"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHLIF_002354"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHLIF_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO9606359.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHLIF_002355"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHLIF_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO9606360.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHLIF_002356"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHLIF_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO9606361.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHLIF_002357"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHLIF_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO9606362.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHLIF_002358"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHLIF_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO9606363.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHLIF_002359"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHLIF_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO9606364.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHLIF_002360"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHLIF_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO9606365.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHLIF_002361"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHLIF_002361"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO9606366.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHLIF_002362"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHLIF_002362"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO9606367.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHLIF_002363"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHLIF_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFO9606368.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHLIF_002364"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHLIF_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO9606369.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHLIF_002365"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHLIF_002365"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO9606370.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHLIF_002366"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHLIF_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO9606371.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHLIF_002367"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHLIF_002367"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO9606372.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHLIF_002368"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHLIF_002368"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO9606373.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHLIF_002369"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHLIF_002369"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO9606374.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHLIF_002370"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHLIF_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO9606375.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="ACHLIF_002371"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="ACHLIF_002371"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO9606376.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="ACHLIF_002372"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="ACHLIF_002372"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO9606377.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="ACHLIF_002373"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="ACHLIF_002373"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO9606378.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHLIF_002374"
     CDS             22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHLIF_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO9606379.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="ACHLIF_002375"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="ACHLIF_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO9606380.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHLIF_002376"
     CDS             23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHLIF_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO9606381.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHLIF_002377"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHLIF_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO9606382.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="ACHLIF_002378"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="ACHLIF_002378"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO9606383.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="ACHLIF_002379"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="ACHLIF_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO9606384.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCVALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTCGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHLIF_002380"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHLIF_002380"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO9606385.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHLIF_002381"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHLIF_002381"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO9606386.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7302 a         8042 c         8484 g         7169 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcat tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gaggggggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaagtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt acgcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga ttccgggtac cgatatgcct gcgcagtact tcctgccggg
    11761 gaaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acacaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt tcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gagttgcgag gcatttagcg
    14881 cccgttctac gtcagcagcc gttttgccgt tgatacgcag gcggatgtcg tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg cgacggcaat
    15121 tgccgtattc actaacaccg cgtcggcccc catttccagc gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg cactgtggcc tgctggataa taatctccag
    15241 catggcgcgg gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcgtttc ggcggctttc agggtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc taattttaac cagtttgtgc ctaacgcttc
    15481 acgagccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgcagagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagacgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacttcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggtgtga tgatgccctg gcgggcgtag tgcagttggg tgacgcggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgctatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcaggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagctg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggctgtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg
    21541 ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag tggctggaag cgatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggat atcgccgatg cgcgccgccg
    21661 cgtgggcaat aaagtcgcgt tgcagggcaa tatggatccg tcgatgctgt acgcgccgcc
    21721 agcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agtggcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc cccgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgcattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgatagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    23761 tagccacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctgc tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatcccg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    24901 cctgctgcgg gtagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 cgcaggaaat gcagcagtta catccgggag atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattatcc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagaag ctagtggcgc
    25741 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    25801 gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg agaagcgcat caactggcgc
    25861 aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt aagcgagctg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aggccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg agcgggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 agagatggac ggcattgcca cgctaaaaga gatcaaagcg ttaaatccgg cgattccggt
    26641 actgattatg accgcatact ccagcgtcga gacggcggta gaggcactga aaaccggggc
    26701 gctggattat ctcatcaagc cgctggattt cgacaccctg caggcgacgc tggaaaaggc
    26761 gctcgcgcat acgcacagta ttgatgctga aacgcctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acggtgctga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgtggc
    27001 actcaacgaa tccttgctgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aggatatccc
    27361 tctgctggct agccattttc ttcagcgttt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ctttacgcca caggcgatgg atttgctgat ccactacgac tggccgggaa acattcgtga
    27481 actggaaaac gcggtggaaa ggtcggtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccagtacgcc gatcccgctg gggcaaagtc aggatattca
    27601 gccgctggtg gaagtcgaaa aagaagtgat tctggcggca ctggagaaaa cgtgcggcaa
    27661 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgttatccgc
    27901 cagtttcgtg cccgcgtgga acactttgcc gcctgccact tcttccagcg gcaggccgtg
    27961 gatcacgtca ccggtgcggt aatcacccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgt tcatcccact ctgacgtttt ctcgtccagt ttgccttcac aggccgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ctggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28321 gcccatcccg cccgtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtggccatc ggcagcacat gctcgccgtc taccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggtcacgcca atagcaacgt tttgcagcgc gggttccagt gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtctcaa ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaataa ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatgg aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcgg cagctttaat accggcgatt ttcgcggagt acacgcggct catctggccc
    29281 gcgccaatac cgatggtcat attgtttttg gcatagacga tagcattgga tttcacgaac
    29341 ttcgccactt tccagcagaa cagcgcatcg cgcagttcct gttcggtcgg ctgacgttgg
    29401 gtgacgacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgctt ggcggcggtg attttcaggg cttcttcgct ggcggaaggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatctagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gctacaccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tcctgcttct taatgaagtt caggttcagc gtgcgtggga agcgaccagc ggcttctttg
    30001 ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt agcggcggag cgcaccatgg tcgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttatttttcg cccacattgt
    30721 aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt caatggtgat cacaattttg
    30781 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    30841 gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc agtcagtcat ttttctgcaa
    30901 tttttctatt gcggcctgcg gagaactccc tataatgcgc ctccatcgac acggcggatg
    30961 tgaatcactt cacacaaaca gccggttcgg ttgaaga
//
LOCUS       DAZGOB010000049        29856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGOB010000049 DAZGOB010000000
VERSION     DAZGOB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.049"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            96..647
                     /gene="ygiL"
                     /locus_tag="ACHLIF_002382"
     CDS             96..647
                     /gene="ygiL"
                     /locus_tag="ACHLIF_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO9606387.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            707..3211
                     /locus_tag="ACHLIF_002383"
     CDS             707..3211
                     /locus_tag="ACHLIF_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9606388.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3227..3976
                     /gene="yqiH"
                     /locus_tag="ACHLIF_002384"
     CDS             3227..3976
                     /gene="yqiH"
                     /locus_tag="ACHLIF_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9606389.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3978..5043
                     /locus_tag="ACHLIF_002385"
                     /pseudo
     CDS             3978..5043
                     /locus_tag="ACHLIF_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5086..5286)
                     /gene="glgS"
                     /locus_tag="ACHLIF_002386"
     CDS             complement(5086..5286)
                     /gene="glgS"
                     /locus_tag="ACHLIF_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO9606390.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            5555..6184
                     /gene="yqiJ"
                     /locus_tag="ACHLIF_002387"
     CDS             5555..6184
                     /gene="yqiJ"
                     /locus_tag="ACHLIF_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO9606391.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            6211..7872
                     /gene="yqiK"
                     /locus_tag="ACHLIF_002388"
                     /pseudo
     CDS             6211..7872
                     /gene="yqiK"
                     /locus_tag="ACHLIF_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
     gene            8111..8170
                     /locus_tag="ACHLIF_002389"
     CDS             8111..8170
                     /locus_tag="ACHLIF_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9606392.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            8487..8546
                     /gene="ibsE"
                     /locus_tag="ACHLIF_002390"
     CDS             8487..8546
                     /gene="ibsE"
                     /locus_tag="ACHLIF_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO9606393.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(8666..10099)
                     /gene="hldE"
                     /locus_tag="ACHLIF_002391"
     CDS             complement(8666..10099)
                     /gene="hldE"
                     /locus_tag="ACHLIF_002391"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO9606394.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(10147..12987)
                     /gene="glnE"
                     /locus_tag="ACHLIF_002392"
     CDS             complement(10147..12987)
                     /gene="glnE"
                     /locus_tag="ACHLIF_002392"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO9606395.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(13010..14311)
                     /gene="ygiF"
                     /locus_tag="ACHLIF_002393"
     CDS             complement(13010..14311)
                     /gene="ygiF"
                     /locus_tag="ACHLIF_002393"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO9606396.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            14553..15173
                     /gene="ygiM"
                     /locus_tag="ACHLIF_002394"
     CDS             14553..15173
                     /gene="ygiM"
                     /locus_tag="ACHLIF_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO9606397.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            15237..16475
                     /gene="cca"
                     /locus_tag="ACHLIF_002395"
     CDS             15237..16475
                     /gene="cca"
                     /locus_tag="ACHLIF_002395"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO9606398.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPLTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(16638..17459)
                     /gene="bacA"
                     /locus_tag="ACHLIF_002396"
     CDS             complement(16638..17459)
                     /gene="bacA"
                     /locus_tag="ACHLIF_002396"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO9606399.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(17549..17917)
                     /gene="folB"
                     /locus_tag="ACHLIF_002397"
     CDS             complement(17549..17917)
                     /gene="folB"
                     /locus_tag="ACHLIF_002397"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO9606400.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            18022..18639
                     /gene="plsY"
                     /locus_tag="ACHLIF_002398"
     CDS             18022..18639
                     /gene="plsY"
                     /locus_tag="ACHLIF_002398"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO9606401.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(18652..19584)
                     /gene="ttdR"
                     /locus_tag="ACHLIF_002399"
                     /gene_synonym="dan"
     CDS             complement(18652..19584)
                     /gene="ttdR"
                     /locus_tag="ACHLIF_002399"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO9606402.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            19791..20702
                     /gene="ttdA"
                     /locus_tag="ACHLIF_002400"
     CDS             19791..20702
                     /gene="ttdA"
                     /locus_tag="ACHLIF_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO9606403.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            20699..21304
                     /gene="ttdB"
                     /locus_tag="ACHLIF_002401"
     CDS             20699..21304
                     /gene="ttdB"
                     /locus_tag="ACHLIF_002401"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO9606404.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            21352..22815
                     /gene="ttdT"
                     /locus_tag="ACHLIF_002402"
     CDS             21352..22815
                     /gene="ttdT"
                     /locus_tag="ACHLIF_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO9606405.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(22858..23871)
                     /gene="tsaD"
                     /locus_tag="ACHLIF_002403"
     CDS             complement(22858..23871)
                     /gene="tsaD"
                     /locus_tag="ACHLIF_002403"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO9606406.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            24109..24324
                     /gene="rpsU"
                     /locus_tag="ACHLIF_002404"
     CDS             24109..24324
                     /gene="rpsU"
                     /locus_tag="ACHLIF_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO9606407.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            24435..26180
                     /gene="dnaG"
                     /locus_tag="ACHLIF_002405"
     CDS             24435..26180
                     /gene="dnaG"
                     /locus_tag="ACHLIF_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO9606408.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNLELAKK"
     gene            26375..28216
                     /gene="rpoD"
                     /locus_tag="ACHLIF_002406"
     CDS             26375..28216
                     /gene="rpoD"
                     /locus_tag="ACHLIF_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO9606409.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(28295..28801)
                     /gene="mug"
                     /locus_tag="ACHLIF_002407"
     CDS             complement(28295..28801)
                     /gene="mug"
                     /locus_tag="ACHLIF_002407"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO9606410.1"
                     /translation="MVEDILALGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            28926..29001
                     /locus_tag="ACHLIF_002408"
     tRNA            28926..29001
                     /locus_tag="ACHLIF_002408"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28959..28961,aa:Ile,seq:cat)
     gene            complement(29055..29819)
                     /gene="nfeF"
                     /locus_tag="ACHLIF_002409"
                     /gene_synonym="yqjH"
     CDS             complement(29055..29819)
                     /gene="nfeF"
                     /locus_tag="ACHLIF_002409"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO9606411.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPLSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT         7597 a         7796 c         7318 g         7145 t
ORIGIN      
        1 ccgaaaagta aaatataata tttgaatgat tagcaaccct taaataacag ttactaatca
       61 ttcattttct acttatattt atataaagga atattatgtc tgcttttaaa aagtcacttc
      121 ttgttgctgg cgtagcaatg attctttcca ataacgtatt tgctgacgaa ggtcacggca
      181 ttgttaaatt taaaggtgaa gttatctctg ctccatgttc aattaaacca ggtgacgaag
      241 acttaactgt aaatctgggt gaagtagcag ataccgttct gaaaagcgac cagaaatctt
      301 tggctgagcc tttcactatt cacctgcaag actgcatgtt gagccagggt ggaaccactt
      361 attcaaaagc gaaggttact ttcaccacag cgaatactat gacgggtcag actgacctgc
      421 tgaaaaacac caaagaaact gaaatcggtg gtgccacagg cgttggcgta cgtattctgg
      481 atagccagag cggcgaagtg actttaggta ctccggtagt aattacattc aacaatacta
      541 acagctatca ggaactgaac ttcaaagctc gtatggaatc cccaagcaaa gatgctaccc
      601 cgggcaacgt ttacgctcag gctgactata agattgctta cgagtaatca cattaggccg
      661 ggttcgcccg gccacatcat taacggatta atgataagtg gaacagatgt ataaaaaatt
      721 aaaattaacc acaataagcg aattgattaa aaatatttat tgttcattat ccgttctcat
      781 cattggttgt gcgtcagctt atgccgttga attcaacaaa gatttaatcg aagccgaaga
      841 tcgtgaaaac gttaaccttt cccaatttga aactgatggc caattacccg taggtaaata
      901 ctcattaaac gctcttatta ataataaaag gacgccaatc catctggacc tgcaatgggt
      961 attaattgat aaccaaactg cagtttgtct gactccagag caattaacat tattaggctt
     1021 tactgatgag attattgaag aggctcagca aaacctgatc gatggttgtt accctatcga
     1081 aaaagaaaaa caaattacaa cttatctcga taaagggaaa atgcaattat ccatatctgc
     1141 acctcaggca tggttaaaat acaaagatgc aaactggacg cctcctgaac tttgggatca
     1201 tggtattgct ggggcatttc ttgactacaa tttatatgcc tctcattatg caccacatca
     1261 gggcgataat tcacaaaata taagttccta tgggcaggct ggggttaatc ttggggcctg
     1321 gcgcctgcgt actgattacc agtacgatca gtcatttaac aatggcaaaa gccaggcgaa
     1381 caacctggat tttccgcgta tttatttgtt tcgcccaatc ccagcaatta atgcaaaact
     1441 aactataggt caatacgata ctgaatcctc tattttcgac tctttccatt tttctggcgt
     1501 ttcgttgaaa agcgatgaga atatgttgcc gcctgatcta cgtggttacg caccgcaaat
     1561 cacgggtgtt gcacaaacga atgcaaaggt cactgtctca cagaacaacc gtattattta
     1621 tcaagaaaat gttcctcctg gcccatttgc tattactaat ttattcaata cattacaggg
     1681 gcaactggac gtcaaggttg aagaagagga cgggcaagtc acgcaatggc aagttgcatc
     1741 taatagtatt ccttatctga cgcgtaaagg gcagattcgc tacaccactg ctatgggtaa
     1801 accgaccagc gttggtggtg attccttaca acaacccttc ttctggactg gtgaattctc
     1861 atggggttgg ctgaacaatg tatccctgta tggtggttca gttttaacaa accgtgatta
     1921 tcaatctctg gctgccggcg ttggctttaa ccttaactca ttgggttcat tatcttttga
     1981 tgtcacacga tctgatgctc agttgcataa tcaggataaa gaaacgggtt atagctaccg
     2041 cgctaactat tcaaaacgtt ttgaatctac cggtagccag ctcactttcg ctggttaccg
     2101 tttctctgat aaaaactttg tgacaatgaa tgaatatatc aatgacacta accattacac
     2161 gaattatcag aatgaaaaag agagttatat tgtcacgttt aaccagtatc ttgaatcatt
     2221 aaggttaaat acatacgtaa gtttggctcg taatacttac tgggacgcca gcagtaatgt
     2281 gaattattca ttatcactta gccgcgattt tgatatcggg ccattaaaaa acgtctccac
     2341 ttctctaaca tttagccgaa taaactggga agaagacaac caggatcaac tgtacctaaa
     2401 tatttcgatt ccctggggaa ccagtagaac attgagctat ggtatgcaac gaaatcagga
     2461 taacaagatt tcgcatactg cttcgtggta tgactcttcc gatcgaaata attcctggag
     2521 cgtttctgct tcaggcgaca atgatgaatt caaagatatg aaagcgtcac tacgcgccag
     2581 ttatcagcat aataccgaga acggtcgtct ctatctctcc gggacatcac agcgagacag
     2641 ttattattct ctgaatgcca gttggaatgg ttcattcacg gcgactcgtc acggagccgc
     2701 tttccatgac tatagtggta gtgctgactc gcgttttatg atcgacgcag atggtgcaga
     2761 agatattccg ttgaacaata aacgcgcggt aactaatcgg tatggcatcg gagttattcc
     2821 atcagtcagc agttacataa caacatcatt aagcgttgat acccgaaatc ttccagaaaa
     2881 tgtggatatc gaaaactcgg ttatcaccac caccttaacc gaaggtgcta ttggctacgc
     2941 caaacttgat acccgcaagg gctatcaaat catgggggtt attcgcctgg cagatggtag
     3001 tcacccacca ctggggatta gcgtaaaaga taaaaccagc cataaagaat taggactggt
     3061 tgctgatggc ggctttgtat acctcaacgg cattcaggat gacagcaaac ttactttacg
     3121 ctggggtgat aaatcctgtt ttattcaacc acccaatagc agcaacttaa ccaccggaac
     3181 ggttatttta ccgtgtatta gccaaaatta attcaggatg aaactaatgc gctacttaaa
     3241 cacaaagaat ctcattgcag caggagtatt actcgcctgc atgagcagca ttgcctgggg
     3301 tgccatcatc ccggaccgta cccgtatcat aatgaatgag tcagataaag gggaagcatt
     3361 aaaacttact aatcaaagca aaaatctacc ctatctggcg caaacatgga tagaagatac
     3421 gaaaggcaat aaatcacgtg actttattgt cacggttccc cctatggtac gtttaaatcc
     3481 cagtgagcaa attcaaataa gaatgataag tcaggaaaaa attgctcaac tgcctaatga
     3541 cagagaaacg ctcttctatt ttaacgtgcg agaaataccg ccaaaaacag ataaaaaaaa
     3601 tgtgatgcaa gtcactatgc agcacgcatt gaaactattc tggcgaccaa aagctattga
     3661 attagaagat gatggggtaa tgtcttacga aaaagttgaa atcatccgta gaaatgatgg
     3721 ttcgatccgc tttaacaata agatgcctta tcacgttacg ctgggttata tcggaacaaa
     3781 cggaatcaca atgttgccac aaacgcaaag tttaatggta accccttata gccatgcaga
     3841 tacgcagttt aaaaatgttc cgtcagcttt tcaggttggt tatatcaatg attttggtgg
     3901 attaagtttt tatgaaataa actgcccgac ggttaataac tcttgtaatg tttccgtagc
     3961 caaacgagat aaataatatg cgctacttat taattgtaat tatatttatt ataggtttta
     4021 gtgcattacc tgtatgggca atggactgct atgctgaaca tgaaggggga aataccgtag
     4081 tcattggtta cgtaccaaga atttctatcc ccagtaatgg taaaaaaggt gataagatct
     4141 ggcaaagcag tgagtatttt atgaatgtct tctgtaataa tgcattacct gccccatcac
     4201 ctggagaaga atacccatca gcctggacta atataatgat gtttttacca ggcggtcagg
     4261 acttttataa tcagaactcc tatatttttg gcgtaacata caatggagtt gattatgatt
     4321 cgactgcccc taatgctatc gcagcaccag aatgcattga tataaaagga gcgggtacct
     4381 ttaataatca ctataaaaaa ccccgctgtc tgtagtggag gtcctgagcc tcaattatca
     4441 gtaacctttc cagctcgcgt acaactctat attaaattag caaaaaatgc caatagagtt
     4501 aataaaaacc tggtgttacc tgatgaatat atcgctctag agtttaaagg tatgagtggc
     4561 gcaggagcta tagaagtaga caaaaatttg accttcagga ttcgtggatt aaataatatt
     4621 catgtccttg actgctttgt taatgtcgca ctggaaccag ctgatggcgt tgttgacttt
     4681 ggtaaaataa attcccgaac aattaaaaat accagcgtta gtgagacgtt tagtgtagtc
     4741 atgacaaaag atcctggtgc ggcctgtact gagcaattca atattttagg gagctttttc
     4801 actacggata ttttgagtga ttatagccat ctggatatgg gtaatggact gctattgaag
     4861 atatttcata aagatggaac agcaacggaa tttaaccgct tctcacaatt tgcttctttt
     4921 tcatcgtcta gtgcaccttc ggtcaccgca ccattcaggg cagaacttag tgcgaatccg
     4981 gcagagactg ttgttgaggg accgtttagt aaagacgtca tcctcaaaat cacctataac
     5041 tagcatctaa tacaaacact aaaacgggcc atcaggcccg ttatatcagt gctctaactc
     5101 cagctctctt gcctgcatca tctgttgaca ataccaggca taacgtgcgc aaaaccaggt
     5161 tctatgttct tcatccatgt taccagtaac ggcaagaata tcgaccgggc gaccttctgc
     5221 gtgcattctg gcaaaactac gcgccaggaa atcgaaatta tttaaagaat taagactatg
     5281 atccataagc acatcctccg gttatcgcat accttttaag gccatatgct ttttctgatt
     5341 tcagtattcc ccaatcagtg tagtgccttt aaacataacg tgcgtaaata tattgtcccc
     5401 gatcacactt tttagtgaaa aataaaaaac catattaaaa aactttctct tccagaactt
     5461 ctctaacatg tgttattatt aacaaagata aatgtaagca aggaaactat cccaaagtaa
     5521 atattgcgtc actaaatgga cattggagtg acatatgatt ttattcgccg actataatac
     5581 cccttatctt ttcgctattt cctttgtact gttgatcggc cttttagaaa ttttagcgct
     5641 tatctgcggt cacatgctct ccggtgctct tgatgcacat cttgaccatt atgattccat
     5701 aaccacaggt catattagcc aggcacttca ttatctcaat atcggaagat tgccagctct
     5761 cgtcgttctc tgcttgctgg ctggtttttt tggtcttatt ggcatcctgt tgcagcatgc
     5821 ctgcatcatg gtctggcaat caccactgtc gaacctgttc gtggttcctg tcagtttact
     5881 gtttacgatt attgcggtgc attataccgg taaaatagtt gcgccctgga ttccacgcga
     5941 ccatagttca gctattacag aagaagaata tattggtagc atggcattaa ttaccggtca
     6001 tcaagccact tcaggtaatc cctgcgaagg aaaactcacc gatcaatttg gccaaataca
     6061 ttatttacta ctggagcctg aagaaggaaa atttttcact aaaggagaca aagtgttaat
     6121 tatttgccga ctttctgcaa cgcgatattt ggcggaaaac aatccctggc cacaaatatt
     6181 gtaatttatt aatcaaaagg aaatttaata atggatgata ttgttaattc tgtgccctcc
     6241 tggatgttta ccgcgattat tgccgtatgc attctgttta ttattggaat tattttcgcc
     6301 aggctctatc gtcgcgcttc ggcagagcaa gcttttgttc gtactggttt aggtgggcaa
     6361 aaagtggtga tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat aatccccatc
     6421 aatatgaata ctctgaagct ggaagtcagc cgctcaacca ttgatagcct gattacgaaa
     6481 gatcgtatgc gtgtcgatgt agtagtcgct ttctttgtac gggtaaaacc gtctgtagaa
     6541 ggcatcgcca ctgcggcgca gacgttgggg caacgcacgc tatcaccaga agacttacgt
     6601 atgttggttg aagataaatt tgtcgatgcc ctccgtgcaa cagctgcaca aatgaccatg
     6661 catgagttac aggatacccg cgagaacttc gtgcagggag tacaaaatac tgtcgcagaa
     6721 gacctgtcga aaaacggtct tgagctggaa agcgtttcac ttaccaactt taaccagacc
     6781 tcgaaagaac atttcaatcc gaacaatgcc tttgacgccg aaggtttaac caaactgact
     6841 caggagacgg agcgccgtcg tcgcgaacgt aacgaagttg aacaggatgt agaagttgcg
     6901 gtgcgtgaga aaaaccgtga tgcactttcg cgcaagttgg agattgaaca acaagaagcg
     6961 tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca gaatgcacgt
     7021 attgcggctt ttgaagctga acgtcgtcgt gaagcagagt agacgcgaat tctggctgaa
     7081 cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag aaaggttgag
     7141 gctgaacgtg aagttcgtat taaagagatc gaacaacagc aggtcaccga aatcgccaac
     7201 cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc agaagcgcgt
     7261 gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac cactcgccag
     7321 accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca ggatgcagaa
     7381 accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc agagatgcag
     7441 gcggcggcta tcgttgagtt agccgaagct acacgtaaaa agggtctggc ggaagcagaa
     7501 gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac cagccttaaa
     7561 ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt tgagccgatg
     7621 aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg cagcgctgcg
     7681 ggtgatgcaa acacgggtaa tgtggggggc ggaaacctgg cggaacaagc attatcagcc
     7741 gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga aattggcgtt
     7801 tcaggcggct cactggcggc attgacttca cccttaacct caacaactcc cgtcgccgaa
     7861 aacgtagaat aaattttcag ctatgaacgt ccctgtcaca aaaagccggg gattttttat
     7921 atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg cgtgaccgct
     7981 cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag gtccggcgct catcaacaat
     8041 cggggagtca gcaaagggct gaaacgggaa agcccctccc taggaagggg ccttacagaa
     8101 ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttaatcg taagtttcgc
     8161 agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt tcctttactt
     8221 taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct ggcccaaaaa
     8281 gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg acgttgcagc
     8341 gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg cgcggggtcc
     8401 ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc cctcccgagg
     8461 aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg gttgtgttgt
     8521 tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat cctcccttac
     8581 ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc ctgctacgaa
     8641 gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc ttcttgatga
     8701 tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg ccgttggccc
     8761 agacttcttt actcccggcg atctcttctg gtttatagtc gccgcccttc accagtaaat
     8821 ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac accacccagt
     8881 cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta acggggcggg
     8941 aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc agacggtcac
     9001 ccagtttgcg agcatttgcc agataggaga cgtgcccggc gtgcaggatg tcaaagacac
     9061 cgttggtcat taccaccttt tcgccgcgtt tacgcgctgc cgctacagca agcttcagtt
     9121 cctcttcggt catcacacca aagccggtat ctgcgcgccc acgcactgca ttttccagct
     9181 cgatcggcga aaccgtggag gttcccagtt tgccgaccac cacgccagcc gccgcattgg
     9241 caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc aggacgccaa
     9301 tcaccgtgtc gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt ggcatatgca
     9361 gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc actaacagag
     9421 ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct tcttcggtct
     9481 tacatttacc gacaacagct tcaaattccg agagattcgg tgtcagcagc gtagcgccgc
     9541 ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacgccc gctttacgcg
     9601 ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca gaaagcacca
     9661 gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg cagcggctgc ggatcaacac
     9721 cttcgaaacc ttcttcaaaa tccaggcgga tcagctgttg gttgcgggaa agtacccgta
     9781 atttggtgat agtcggatgc gtcggtacag aaacgaagtc gcatttgacg ttgacgtcgg
     9841 ccagagattt actcagcgca cgcgccgcat cgtcaatgcc cgtcagcccg accaggcgag
     9901 cattggcacc gagagaagcg atgttcattg ccacgttagc cgcgccgccc ggacgttctt
     9961 cgatggtatt gactttaacc acgggcaccg gcgcttccgg cgagatacgg ctggtgggac
    10021 cgtaccagta acgatccagc atcacatcac caaccaccat cactcctgca cgttcaaact
    10081 ctggcagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc gcgataatac
    10141 catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagtt cacgctctgc
    10201 ggtgaagcaa tcctccggca catggcccgg caattcctgt aatgccagat gatgaagttc
    10261 atcgcgcagc gtagtatacg cacgggtcag cgccatcgct tcctgctctt ccataatgtc
    10321 gttttgcgcc agtagttcca gaatgcgcac gttgtctgac cagcgcgtta acttcggttt
    10381 ttcatgagcg tagcgcaaca ccagatattg ggtaataaat tcgatatcgg taattccgcc
    10441 ttcatcagct ttgatatcaa agcgatcgcg atgtttattg ccgagatgag cgcgcatttt
    10501 ctcgcgcatt tcccgcactt ccgtttgcag agttttacct tcacgcggca gcgtcataat
    10561 ctcgcgacgc accgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca ctacccgcgc
    10621 acgcaccagc gcctgatgtt cccacgtcca ggcctcgttt ttctgataat cggcaaatgc
    10681 ttctgcggat gtcaccagca ttcccgcggc cccggacggg cgtagccgtg catccacttc
    10741 atacaaaatg ccggaagagg tacgcgtact gaacagatgc ataatgcgtt gcgccagacg
    10801 cagataaaac tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat ccattgggca
    10861 atcatggagg aagatcaggt caagatcgga actgtagcct aactcccagc cgcccagctt
    10921 gccgtagccg accaccgcaa aaccacggcc ttcgcgatcg ttgaggtgat ttggcttacc
    10981 gtagcgggcc accatttgaa cccacgcctg ctgaacgacg gcatctatca tagcctctgc
    11041 cagccaggtt aagtgatcgc tcactttcat caccggtagc gtaccagcga tatccgctgc
    11101 ggcgatgcgt aacagctgtg cctgtttgaa ctgacgcagc gcctcaagct gttgctcttc
    11161 gtcatcttcc ggcacgcgca gcaaatactg gcgcaactca tcgcggtagg catcggtcgc
    11221 cgtcggctgg taaagggtgt ttggatcgag caattcatcc agcaataatg gataacgcgc
    11281 cagttggctg gcaatcatcg gcgacgcggc acacagagaa atcaaatgtt taagcgccgc
    11341 ggggaattca ctgagcaatt ctaaataggt ggtgcgggta acaatcccca ccagcaaggc
    11401 ggtaatgcgc gacagcgtaa cggcagcgtc ttcacgcgca cagacatcac ttagcagatg
    11461 cggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atggtgcgct tatccagctc
    11521 tttgcggaaa tcggcaatca gcgttagcac ctgtttgcga tcatcctcgc taagatgcgc
    11581 cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac gccactgttc
    11641 cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat taaacacccg
    11701 gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt cagcaaagtc
    11761 catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgtct gggtttgttc
    11821 gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatacg ccactcgcaa
    11881 ttgttcagca tcgttttcag aaagcagatg cagcgcggca atgacgctga gcgttggcag
    11941 taaagagcgc gactgcagcg acggttcgcg cccaccgcga atgagctgga acacctgaac
    12001 gataaattca atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg tcaaaccacg
    12061 tcgacgcact tcacgggcaa tcatcccttt catgttgcgc agcgactgaa tcacgctgaa
    12121 atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt tagcatagac
    12181 gccttcgcta tcgcccataa tccgcgcctt gaccatcgcg taacgctccc agtcgcgccc
    12241 ctgctcctgg taataatcct ccaacgcggc aaagctcagc accagcgggc cactttcgcc
    12301 aaacggacgc agccgcatat ccacgcgata gacgaagcca tcctgcgttg gttgatccaa
    12361 cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca gttcccggcg
    12421 tccgccctgc gtacaaccat gttccggcca ggcaaaaatc agatcgatat ctgaggagaa
    12481 attcagttcc ccaccgccca gcttacccat gcctaaaatc agcagcggtt gcgcttcgcc
    12541 ctgcgcattg cacggcgttc cccactcgcg gcagcaagcg tcatacagcc agtcacgcgc
    12601 ggcgacaatc agcgtctccg ccagatggct gagctgctgc aatatgctct cttcagtaac
    12661 caaagccagc gtttgcgccc aggcgatgcg caccataatg cgccgccgga atagccgcag
    12721 ctcgcgcatt aacccggctt cgtcactcac attactgagc gcctcctgca accatgccgc
    12781 gtaatgctgc cattcgtcgg cctgcggcgg ttggctttcc agttccgtca gccactctgg
    12841 atgcacaatc acgctgtcct gcacaaaatc actaaaagta agtactgact tcgcctgtgc
    12901 gctaagtgat tcctcggcta aaggctctgg cagccgctca acaacggtct gccagtactg
    12961 ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat taacgttttc
    13021 cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt tcaatttcaa
    13081 tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc caggcttgcg
    13141 cgacgacagg atcatagtaa cctgccagca gcagtattga gtcaatatca cgcgtcaggc
    13201 gtggcaactg atcatggtag cgatcgccta acggctggca gaaaacgctt ttcagttcag
    13261 cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctg attttaccct
    13321 gcgctttggc atctaaaaac ggctgccagg ctttgcttac caaccattcg gtcaacgcca
    13381 gctttgccat tgccgtttcg gtagagtaga ccgccgtcac ggcagaaacc gcagaagcga
    13441 tggtcgcttc gcattgagtc agcaaatcac gtaagtgagt gctcgcttta cgcggcacaa
    13501 tgccaccgaa cagcatcagt gcatggcgaa caaggccaat ggctgccagc acctgctctt
    13561 tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc gctaacgcca
    13621 gctcgaacgc cgcttccagc ccctgttcca catcggcttt tgccgcaaca tgcaaaatgg
    13681 tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca cgcgccgctt
    13741 tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc agtttcagca
    13801 ccgcgcgcgt gtcgccgcta agcagttcca gttccagctc acagataggt tcagcaaatt
    13861 cccccgcttt cacctccccc tgatcgagag cgatttcaat tcggctgtca tcgaccgcca
    13921 ccagccattt ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg gaagcgagat
    13981 cggcgggcaa ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg tcgagcgtcg
    14041 gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc actcttcctg
    14101 caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgtaag cccatatcgt
    14161 gcccacgcag ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc acggggtcat
    14221 ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc ctcaacggca ctgtgattaa
    14281 caataaactt taattcgatt tcctgagcca tggcctttac ttatcggtta tgtcacataa
    14341 gtgacgatga acggcgaact taatgcgatc ttttttgtca gtagatagta ttttgcgcca
    14401 aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg caagacctga
    14461 cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc cactactatc
    14521 gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg atcggattaa
    14581 ctttactcgc acttagcgcc actgccgtct cacacgctga agaaacgcgc tatgtttctg
    14641 atgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc gtgggcacgg
    14701 ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat tatgcccagg
    14761 tgaaagacag ctctggccgt accgcctgga tcccgttgaa acaacttagc actgagccaa
    14821 gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc gataaactca
    14881 ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa gtggcgcaga
    14941 gcgacagcgt gatcaacgga ttaaaagaag aaaatcagaa attgaaaaac gagctgattg
    15001 tcgcgcagaa aaaggtcgat gccgccagcg tacagttgga tgacaaacag cgcaccatca
    15061 tcatgcaatg gtttatgtat ggcggtggcg tgctggggct tggcttgctg ctcggtctgg
    15121 tactgccaca cctgatccca acgcgcaaac gcaaagatcg ctggatgaac taaatcgcct
    15181 tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat gatggcgtga
    15241 agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctaccg gtcaaagaca
    15301 gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc taccagcagg
    15361 taggccgcga ttttcctgtg tttctgcatc cgctaacgca tgaagagtat gcgctggcac
    15421 gtaccgaacg gaaatccggt tccggttaca ccggttttac ttgctatgcc gcaccggatg
    15481 tcacgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg gctcaggacg
    15541 ataacggcga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat cgtctgttgc
    15601 gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt tctgcgcgtg gcgcgttttg
    15661 ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga aactctgacg ctgatgcgcg
    15721 agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg aaagagacgg
    15781 aaagcgccct caccacccgc aatccccagg tgttcttcca ggtactgcgc gattgcggtg
    15841 cacttcgcgt tttattcccg gaaattgacg ccctgtttgg cgttccggcc cctgccaggt
    15901 ggcatccgga aatcgacacg ggtattcata ccttaatgac gctctcaatg gcggcgatgc
    15961 tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca cgatctcggt aaaggactga
    16021 cgccgccaga actttggccg cgtcatcatg ggcatggccc ggcgggtgtt aagttagtgg
    16081 aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc agactggtgg
    16141 ctgagtttca cgatctcatc cacaccttcc caatgctgaa cccaaaaacc atcgtcaaat
    16201 tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg gcgctgacca
    16261 gcgaggctga cgtacgcggc agaaccggtt ttgaatcagc ggactacccg caaggccgtt
    16321 ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc gttgaagcgg
    16381 gatttaaagg tgtggagatt cgcgaggagc tgacccgacg gcggattgcg gcggtagcca
    16441 gttggaagga acaacgttgc ccaaagcctg actgagtata aaactaaacg ttaacacgaa
    16501 ccatcccctc gccccttcgg ggagagggtt agggtgaggg gaacagacca gcaccggtgc
    16561 gaacaatcac ccccaccccg gccctctccc tacagagcga gggagaaaac caggcctgct
    16621 cccgaacctt ttctcaatca aaagaacacg acgtacaccg cagccgccac aataaagcga
    16681 taaatggcga acgggataaa cgaaatgcgc ttaatcaatt gcaggaaggt tttaatcgct
    16741 atcagcgcca ccacaaaagc ggtgataaac ccaacggcaa acatcgggat atcgccggtt
    16801 gtcaggaagc cccagctttt gtagagatcg agcgccgttg cgcccatcat catcggtacc
    16861 gccagcagga acgagaactc ggaagcagcg taacggctca cgcccatcag catcccacct
    16921 gaaatggtgg ccccggaacg ggagaaaccc ggccacagcg ccagacactg gaaacagcca
    16981 atcataaacg cctgacgata ggtcatatca tcaagacccg gcgcacgcgg ctctttcggc
    17041 ttcaggcatt cggcggcaat cagcaacaaa ccgccaacga ccagtgcata catcacattt
    17101 atcgggttaa acaatgactt aatcgtgtcg tggaacaaca gccccagtac taccgccgga
    17161 atcatcccca gcaaaatgtg gatcagcgtt aaacgacctt tgctttctcc ttcgtgctgc
    17221 aacgggcggc caaagtggat gccaatcagg ccaaacagac gccgccagaa catcactact
    17281 accgccagaa ttgatcctaa ctggatcaca acttcaaagg ttttcgccgt gtcgccctcg
    17341 aaccccaaca agtgaccgac aataatcata tggcccgtgc tggataccgg cagaaattct
    17401 gtcaatcctt cgaccacacc caatattgcc gctatcagca gcgagtgcat atcgctcatc
    17461 aataaacccc taaattatta aaatgtaccg cttgtccgaa ctactgcgta tgaccaggtt
    17521 ataaccgttt ggtttaacag ctgtaaaatt aattattttc tttcagatta ttgccacgct
    17581 caatgattac gccaacattc gccgcccgcg ccactgcgcc tggcttgctg agtttgatac
    17641 gcacccacgg cgagttaaag cgtgctaaca gcagctccgc cacctcttca gccacgcgtt
    17701 ccaccagcgc aaaacgcgcc ccctcgacgt ggctgaccac cgtttctgca atgtcagcgt
    17761 aactgaggca atccgccaca tcatcacttt tcgccgcttt acggttatcc catgccattt
    17821 cgatatcgaa cactaacttc tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg
    17881 tgattaccga aagttgctct ataaatacaa tatccatcac gtcctgcctg ctttttggct
    17941 aaccggatac cacttccggc gaaatgtgcg tattatccac agattcatcg ttgaacacga
    18001 attttcaaaa cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac
    18061 ctctgcggct ccatttccag tgccattctg gtttgccgct tgtgtgggct gcccgatccg
    18121 cgaaccagcg gctccggcaa tccaggcgca accaatgtgt tacgtatcgg tggcaaggga
    18181 gcagccgtag cagtactgat tttcgacgtt ctgaaaggaa tgttgcccgt ctggggcgcg
    18241 tatgaattag gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga
    18301 cacatctggc ccgttttctt cggatttaaa ggaggaaaag gcgttgctac cgcttttggt
    18361 gccatcgcac ccattggctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc
    18421 gtgctattga gcggatactc gtcgctggga gcgattgtca gtgcactgat tgctccgttt
    18481 tatgtatggt ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc
    18541 ctgctgcgtc atcatgacaa catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg
    18601 acgaaattca aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga tctacatgac
    18661 ctgatagcct tcatcgggct tgcccagccg ttgctggcac catgccgcca gaaattccac
    18721 gcagacacgt aatttcatgc tgcgatagag cggctcccgg taaacagccc agatattggc
    18781 gctttgtgca tactccggca atacttgcac cagtttgcca ctctccagaa acggcaacac
    18841 atcccactcg gaacgcagca taatcccttt gccctccagc gcccattgca acactatctc
    18901 gccgctattg gaggaaagat gcccgctaac cttcaccgat tttttctcct gaccgttccc
    18961 caactcccat attccgtggg tcatatcgcg ctctttggtc accagacagt catgacgact
    19021 taattcctgt aatgattgtg gctgcggata tttttgcaga tactcaggtg ctgcgcataa
    19081 tattctttta tttttcgtta acagatgcgc aatataataa tcaggaattt cgtcattaat
    19141 acgaatatcc agatcaatat tatcctgcac taaatcaatt tgccgatcga acaattcaaa
    19201 atgcacctgt aactcaggat aattgcgcat aagttcggta atggctggcg caatatggct
    19261 gcgcccaaaa ccaaaactgc aaccaatacg aatcatcccc tccgggcgcg ttttgatttg
    19321 cgtgacgtca tcgaccagtc gctgatattg ggtgaggatt tccagcgcat gttcgtaaca
    19381 acgttggcca ctttcagtca gcgccacgcc gcgagcggag cggttcagca gcgttgttgc
    19441 cagcgtattt tcaaggattt ggatccgctt ggtgacaaat gcaggcgtct gcccaagcgt
    19501 cgccgctgca gcactaaaac tcccgctgtg gacgatttct accagtacct gcaagtcttt
    19561 ggctaaaggc cagctattca gcatcgttaa ttatccgcag ttgtgataag cgcagtgtat
    19621 ttcgcaaaac attgccattc cctgctctgg cgcaatattc attcttattt tcgcgttgat
    19681 tcacgaactg ttaattacat attcacggtg gcaaaaaata taaaaccaca tttttagtgg
    19741 tagtttgtgg cggtgaattt ttccagacaa atacaaaaac tggagttgcc atgatgagcg
    19801 aaagtaataa gcaacaggca gtgaataagt tgacagagat tgtcgctaac tttaccgcca
    19861 tgatttctac ccgaatgcct gatgacgtgg tggataaact aaaacagcta aaggatgccg
    19921 aaacgtcgtc gatggggaaa attatctacc atacgatgtt cgacaacatg caaaaagcga
    19981 ttgacctgaa tcgtcctgcc tgtcaggaca ccggggagat tatgttcttc gttaaagtcg
    20041 gttcccgctt cccactgctt ggcgagctgc aaagcatact caaacaagcc gtggaagagg
    20101 caaccgtcaa agcgccacta cgtcacaatg cggtagaaat ttttgacgaa gtaaacaccg
    20161 gcaaaaatac cggtagcggc gtaccgtggg tcacctggga catcatcccc gacaatgacg
    20221 atgcggaaat cgaagtttac atggcaggcg gcggctgcac gctacctggc cgctcgaaag
    20281 tgttaatgcc gtcagaaggc tacgaaggcg tggtgaaatt cgtcttcgaa aatatctcca
    20341 ccctcgccgt aaacgcctgt ccaccggtac tggtgggcgt gggcatcgcc acctcggtgg
    20401 aaaccgccgc cgtgctctcg cgaaaagcca ttttgcgccc gattggcagc cgccatccca
    20461 atccaaaagc ggcagagctg gagctacgcc tggaagaagg actcaaccgt ctgggaattg
    20521 gcccacaagg gctgactggc aacagttcag tgatgggcgt gcatatcgaa tctgccgccc
    20581 gccatccgtc aaccatcggc gttgctgttt ctacaggctg ctgggcgcat cgtcgcggca
    20641 cactgctggt tcatgccgat ctcacctttg aaaatctgtc tcacacccgg agcgcgttat
    20701 gaaaaagatc ctgacaaccc cgatcaaagc tgaagatctg caagatattc gcgtcggcga
    20761 tgtgatctac ctgaccggta cgctggtgac ctgccgtgac gtttgtcacc gccgcttgat
    20821 cgaactgaaa cgtccgatcc cttacgatct caacggcaaa gcgattttcc acgctggccc
    20881 catcgtgcgc aaaaacggcg ataaatggga gatggtttcc gttggcccga caaccagtat
    20941 gcgtatggaa agttttgaac gtgaatttat cgagcagacc ggcgtgaaac tggtggttgg
    21001 caaaggtggt atggggccgc tgaccgaaga aggctgccag aaattcaagg cgctacatgt
    21061 gattttcccg gcaggctgcg cggtgctggc ggcaacccag gtagaagaga ttgaagaagt
    21121 gcactggaca gaactcggga tgccggagtc actgtgggtc tgccgggtca aagagttcgg
    21181 cccgctgatt gtctctattg atacccacgg caacaacctg atagccgaaa acaaaaagct
    21241 gttcgccgaa cgccgcgatc ccatcgtgga agagatctgc gagcacgtcc attacatcaa
    21301 ataaccctcc cggagaggct cacccctctc ctttttcgca ggcataacac gatgaaacct
    21361 tccactgaat ggtggcgata tcttgcgccg ctggcggtca tcgccattat tgctctactt
    21421 ccggttcccg cagggctgga gaaccatacc tggctctact ttgccgtttt tactggcgtg
    21481 atcgttggac tgatcctcga acccgtgccg ggtgccgtgg tggcgatggt gggtatctcc
    21541 atcatcgcca tactctctcc ctggctgctg ttcagccccg aacagctcgc ccagccaggc
    21601 tttaaattca ctgcaaaatc cctctcgtgg gcggtttccg gtttttctaa ttcggttatc
    21661 tggctgattt tcgccgcctt tatgtttggc acaggctatg aaaaaaccgg gcttggacgc
    21721 cgcattgcgc tgtttctggt gaaaaagatg ggacatcgca cgctgtttct cggctatgcg
    21781 gtgatgttct ccgagctgat cctcgcacct gtaacaccgt ccaactcggc gcgtggtgcg
    21841 gggattatct atcccatcat ccgtaacctg ccaccgctct atcaatcaca accaaacgac
    21901 agcagttcgc gcagcattgg ctcgtacatc atgtggatgg ggattgttgc cgactgcgtg
    21961 accagcgcca ttttcttgac ggcgatggca ccaaacttgc tgttaattgg actgatgaaa
    22021 agcgcatctc acgccacgct gagttggggc gactggttcc tcgggatgtt gccgctcagt
    22081 attttgctgg tgctgctggt tccctggctg gcttatgtgc tgtacccgcc ggtactgaag
    22141 tctggcgatc aggtgccgcg ctgggcagag acggaactgc aggcaatggg cccgctctgt
    22201 tcgcgtgaaa aacggatgct ggggctgatg gtaggcgcgc tggtgctgtg gattttcggc
    22261 ggtgattata tcgatgccgc gatggtcggt tacagcgtgg tggcactgat gctgcttctg
    22321 cgcattatca gttgggacga cattgtcagt aataaagcgg cgtggaacgt tttcttctgg
    22381 ctggcctcgc ttatcaccct cgctaccgga ctcaacaaca ctggttttat tagctggttt
    22441 ggcaaactgt tagcaggcag cttaagcggt tattcgccaa cgatggtgat ggtggcgttg
    22501 attgtggtgt tttatctgct gcgctacttt ttcgccagcg ccacggcgta tacctccgcc
    22561 ctcgcaccga tgatgattgc cgctgcgctg gcgatgccgg aaatcccgct gccggtgttc
    22621 tgcctgatgg ttggcgcggc aattggtctg gggagcattc ttacaccata cgccaccgga
    22681 cccagtccga tttactacgg tagtggttat ctgccaacgg tggattactg gcgactgggg
    22741 gcgatttttg gactgatatt cctcgtgttg ctggtgatta ccggcttgct gtggatgccc
    22801 gtggtgttgc tttaagtgag gacggtgccg gaaattgctc tccggcaccg gatagtttta
    22861 cgcagccggt aactctgcca gtggccagcg cggacgcacg ctaacgccga gatccgccgt
    22921 cgcgcctgct ttaaaccgca ccattccggc ataggcgatc atcgcgccgt tatcagtaca
    22981 aaattccgga cgcgcgtaga acacttcgcc gcggcgtttt ttcatcattt ccgccagctt
    23041 cgcccgtaac gtgcggttag cactcacgcc gcccgccatg accagtcgct taaagcccgt
    23101 ctgatccaac gctcgcttac acttaatcat caacgtatcg accaccgcat cttcaaaggc
    23161 gcgggcgata tcagcacgcg tctggtcgtc ggtgccgttg tcacgaatcg tgttcgccgc
    23221 aaaggttttc agaccagaaa agctgaaatc cagccccgga cggtcggtca tcggacgcgg
    23281 gaaaacaaag cgcccggcag taccctgagc cgccattttc gacagtaacg gtccgccagg
    23341 ataatccagc cccagcagct tcgcggtttt atcaaacgct tcaccggcgg catcatcgat
    23401 agactcgccg agcagctcgt actgaccaat gccagtcacg ctgattaact gcgtatgacc
    23461 gccggacacc agcagcgcga caaacggaaa ttccggcggg ttatcttcca gcatcggcgc
    23521 taacagatgc ccttccatat ggtgtaccgg gattgccgga acgttccagg caaacgccag
    23581 agaacgcccc acggttgcgc caaccagcag tgcgccgact aatccagggc ctgcggtata
    23641 ggccacagca tcaatgtctt ttgccgttaa accagactcc tttagcgccg cctggatcaa
    23701 cggtacggtt ttgcgcacgt gatcgcggga ggccagttca ggcacgacgc cgccgtagtc
    23761 agcgtgcaat ttcacctgac tatacaattg gttggctaac aaaccttttt catcgtcgta
    23821 aatggcgatg ccggtttcat cgcaggaagt ttcaataccc agtacacgca tgacttgttt
    23881 tacctcgctt tattaccgcg cagtgtagga ccaatgcggg ttgatgtaaa actttgttcg
    23941 cccctggaga aagcctcgtg tatactcctc acccttataa aagtcccttt caaaaaaggc
    24001 cgcggtgctt tacaaagcag cagcaattgc agtaaaattc cgcaccattt tgaaataagc
    24061 tggcgttgat gccagcggca aaccgaatta atcaaaggtg agaggcacat gccggtaatt
    24121 aaagtacgtg aaaacgagcc gttcgacgta gctctgcgtc gcttcaagcg ttcctgcgaa
    24181 aaagcaggtg ttctggcgga agttcgtcgt cgtgagttct atgaaaaacc gactaccgaa
    24241 cgtaagcgcg ctaaagcttc tgcagtgaaa cgtcacgcga agaaactggc tcgcgaaaac
    24301 gcacgccgca ctcgtctgta ctaattcccc gagagcgttg ctctccgatc agaccgagtt
    24361 gtagttgtaa ggccgtgctt ccgaaaggaa tgcgcggctt attttcgttt atgaattgct
    24421 aaaaatcggg gcctatggct ggacgaatcc cacgcgtatt cattaatgat ctgctggcac
    24481 gcactgacat cgtcgatctg atcgatgccc gtgtgaagct gaaaaagcag ggcaagaatt
    24541 tccacgcgtg ttgtccattc cacaacgaga aaaccccgtc attcaccgtt aacggtgaga
    24601 aacagtttta ccactgcttt ggatgtggcg cgcacggcaa cgcgatcgac ttcctgatga
    24661 actacgacaa gctcgagttc gttgaaacgg tcgaagagct ggcagcaatg cacaatcttg
    24721 aagtgccatt tgaagcaggc agcggcccca gccagatcga gcgccatcag aggcaaacgc
    24781 tttatcagtt gatggacggt ctgaatacgt tttaccaaca atctttacaa caacctgttg
    24841 ccacgtctgc gcgccagtat ctggaaaaac gcggattaag ccacgaggtt atcgctcgct
    24901 ttgcgattgg ttttgcgccc cccggctggg acaacgtcct gaagcggttt ggcggcaatc
    24961 cagaaaatcg ccagtcattg attgatgcgg ggatgttggt cactaacgat cagggacgca
    25021 gttacgatcg tttccgcgag cgggtgatgt tccccattcg cgataaacgc ggtcgggtga
    25081 ttggttttgg cgggcgcgtg ctgggcaacg atacccccaa atacctgaac tcgccggaaa
    25141 cagacatttt ccataaaggc cgccagcttt acggtcttta tgaagcgcag caggataacg
    25201 ctgaacccaa tcgtctgctt gtggtcgaag gctatatgga cgtggtggcg ctggcgcaat
    25261 acggcattaa ttacgccgtt gcgtcgttag gtacgtcaac caccgccgat cacatacaac
    25321 tgttgttccg cgcgaccaac aatgtcattt gctgttatga cggcgaccgt gcaggccgcg
    25381 atgccgcatg gcgagcgctg gaaacggcgc tgccttacat gacagacggc cgtcagctac
    25441 gctttatgtt tttgccggat ggcgaagacc ctgacacgct ggtacgaaaa gaaggtaaag
    25501 aagcgtttga agcgcggatg gagcaggcga tgccactctc cgcatttctg tttaacagcc
    25561 tgatgccgca agttgatctg agtacccctg acgggcgcgc acgtttgagt acgctggcac
    25621 tgccattgat atcgcaagtg ccgggcgaaa cgctgcgaat atatcttcgt caggaattag
    25681 gcaacaaatt aggcatactt gatgacagcc agcttgaacg attaatgcca aaagcggcag
    25741 agagcggcgt ttctcgccct gttccgcagc taaaacgcac gaccatgcgt atacttatag
    25801 ggttgctggt gcaaaatcca gaattagcga cgttggtccc gccgcttgag aatctggatg
    25861 aaaataagct ccctggactt ggcttattca gagaactggt caacacttgt ctctcccagc
    25921 caggtctgac caccgggcaa cttttagagc actatcgtgg tacaaataat gctgccaccc
    25981 ttgaaaaact gtcgatgtgg gacgatatag cagataagaa tattgctgag caaaccttca
    26041 ccgactcact caaccatatg tttgattcgc tgcttgaact gcgccaggaa gagttaatcg
    26101 ctcgtgagcg cacgcatggt ttaagcaacg aagaacgcct ggagctctgg acattaaacc
    26161 tggagctggc gaaaaagtga tttaacggct taagtgccga agagcgatcg ggaagccccc
    26221 gacagccgca ctgagaggca gcggcaaata tataagtacg ccctcgtaat tatcgttggc
    26281 ggtaaacaac cgttggattt cagcgttaac ggctgaagga catcgggtca atcgcccaac
    26341 accaacctaa tgaaataagt gtggataccg tcttatggag caaaacccgc agtcacagct
    26401 gaaacttctt gtcacccgtg gtaaggagca aggctatctg acctatgccg aggtcaatga
    26461 ccatctgccg gaagatatcg tcgattcaga tcagatcgaa gacatcatcc aaatgatcaa
    26521 cgacatgggc attcaggtga tggaagaagc accggatgcc gatgatctga tgctggctga
    26581 aaacaccgcg gacgaagatg ctgccgaagc cgccgcgcag gtgctttcca gcgtggaatc
    26641 tgaaatcggg cgcacgactg acccggtacg catgtacatg cgtgaaatgg gcaccgttga
    26701 actgttgacc cgcgaaggcg aaattgacat cgctaagcgt attgaagacg ggatcaacca
    26761 ggttcaatgc tccgttgctg aatatccgga agcgatcacc tatctgctgg aacagtacga
    26821 tcgtgttgaa gcagaagaag cgcgtctgtc cgatctgatc accggctttg ttgacccgaa
    26881 cgcagaagaa gatctggcac ctaccgccac tcacgtcggt tctgagcttt cccaggaaga
    26941 tctggacgat gacgaagatg aagacgaaga agatggcgat gacgacagcg ccgatgatga
    27001 caacagcatc gacccggaac tggctcgcga aaaatttgcg gaactacgcg ctcagtacgt
    27061 tgtaacgcgt gacaccatca aagcgaaagg tcgcagtcac gctgccgctc aggaagagat
    27121 cctgaaactg tctgaagtat tcaaacagtt ccgcctggtg ccgaagcagt ttgactacct
    27181 ggtcaacagc atgcgcgtca tgatggaccg cgttcgtacg caagaacgtc tgatcatgaa
    27241 gctctgcgtt gagcagtgca aaatgccgaa gaaaaacttc attaccctgt ttaccggcaa
    27301 cgaaaccagc gatacctggt tcaacgcggc aattgcgatg aacaagccgt ggtcggaaaa
    27361 actgcacgat gtctctgaag aagtgcatcg cgccctgcag aaactgcagc agattgaaga
    27421 agaaaccggc ctgaccatcg agcaggttaa agatatcaac cgtcgtatgt ccatcggtga
    27481 agcgaaagcc cgccgtgcga agaaagagat ggttgaagcg aacttacgtc tggttatttc
    27541 tatcgccaag aaatacacca accgtggctt gcagttcctt gacctgattc aggaaggcaa
    27601 catcggtctg atgaaagcgg ttgataaatt cgaataccgc cgtggttaca agttctccac
    27661 ctacgcaacc tggtggatcc gtcaggcgat cacccgttct atcgcggatc aggcgcgcac
    27721 catccgtatt ccggtgcata tgattgagac tatcaacaaa ctcaaccgta tttctcgcca
    27781 gatgctgcaa gagatgggcc gtgaaccgac gccggaagaa ctggctgaac gtatgctgat
    27841 gccggaagac aagatccgca aagtgctgaa gatcgccaaa gagccaatct ccatggaaac
    27901 gccgatcggt gatgatgaag attcgcatct gggggatttc atcgaggata ccaccctcga
    27961 gctgccgctg gattctgcga ccaccgaaag cctgcgtgcg gcaacgcacg acgtgctggc
    28021 tggcctgacc gcgcgtgaag cgaaagttct gcgtatgcgt ttcggtatcg atatgaacac
    28081 cgaccacacg ctggaagaag tgggtaaaca gttcgacgtt acccgcgaac gtatccgtca
    28141 gatcgaagcg aaggcgctgc gcaaactgcg tcacccgagc cgttctgaag tgttgcgtag
    28201 cttcctggac gattaatcgg taggccggat caggcgttac gccgcacccg gcactagtcc
    28261 ctttgcacaa acgccacctt ttcggtggcg ttttttatcg cccacgcact accagcgcct
    28321 ggtccagctc gcgatatgct tcaaccagtt tctctagtga aacgcgactt aaaccgctgg
    28381 gatttggcag cacccaaatc tgcgtcgaac caatggtgag cgtttgtttc ccccactgtg
    28441 caccgcgctg gctgaatccc tgttcatatg cttgtttgcc cagaatcgcc aacgcctgcg
    28501 gctgataatc ttcaattttt tcaatcagct tacgcccacc tgcgtgcagc tcctgctttg
    28561 aaacttcatt ggcttgcacc gttggacggt ctaccagttt ggtgacgcca caacgataat
    28621 ccagcagatg ctgtgcctcc tgcggcttca actgacggtc ggtaaacccg gcctgatata
    28681 tcaccttcca gaagcgattt gctggatgag caaagggaaa accagtcccg gcggatgaaa
    28741 gcccagggtt gataccgcaa aacacgaccc gtaaccctag agccaaaata tcctcaacca
    28801 tatttactcc tttctaacat caccaggaaa agtataacgg attgaaaata cattgtttat
    28861 aaaaacagca ggcgcacggt aatggctgga ttgcgacacg gagttacttt ataatccgct
    28921 accatggccc cttagctcag tggttagagc aggcgactca taatcgcttg gtcgctggtt
    28981 caagtccagc aggggccacc agatatagca aaggctgacg agaaatcgtc agcctttttc
    29041 tttttatata tcagttactt tgcgtgccag taagccgctg cacgtacccg ctgtgggtca
    29101 tactgttccg cttcaaagcg gcggcttaaa ttcttaacga ctttaccttc gccggttatc
    29161 cagatgaagt aatcatcggc agggatttgc atctgcgcca gacgcgcatc taccgcctgc
    29221 tcatcatgtg ccagccattc gatattaaaa ccatcaaggt gcgcgagata atcctgacag
    29281 gcgttatccc gcacgctaac cagcgcacta acttgcggtt taacggcaag tttgctcaac
    29341 gtttccaggc ggcggcgcag tgcgggcatt ccggattcat cgcagacata cagctgatac
    29401 gcgtaatctt ccggcaccac cagcgaaccg cgcggacctg ccaccgtaag tttatcgccc
    29461 ggttgcgcct gcatcgccca gccgctggcg accccaccgt cgtgaataaa gaaatcgatc
    29521 gccagttcat ggcgtagttc gtcatacagc ggcgtatagt cacgcgacag tgggcgtggt
    29581 ccttccggcc agacgatgcc ctcttccgtt accgttggcg gcacaaagtg agcgtcaggt
    29641 tgaggaaaga agagtttgct gtgatcgtca aagccgcgcg atgtaaaacc gtccagcgcc
    29701 tcgccgccga ggacaatgcg ctgaaaaccg gcgctgatgc gctcaacgcg taacacagtc
    29761 agttcacgga agcgcagatc attgcgaacg cgctgcgggt agcgggggga gttattcatt
    29821 gttatcgcct tcgtgatagt aatcagatat atctaa
//
LOCUS       DAZGOB010000050        29713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGOB010000050 DAZGOB010000000
VERSION     DAZGOB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.050"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(115..1944)
                     /gene="glmS"
                     /locus_tag="ACHLIF_002410"
     CDS             complement(115..1944)
                     /gene="glmS"
                     /locus_tag="ACHLIF_002410"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO9606412.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2106..3476)
                     /gene="glmU"
                     /locus_tag="ACHLIF_002411"
     CDS             complement(2106..3476)
                     /gene="glmU"
                     /locus_tag="ACHLIF_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO9606413.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3827..4246)
                     /gene="atpC"
                     /locus_tag="ACHLIF_002412"
     CDS             complement(3827..4246)
                     /gene="atpC"
                     /locus_tag="ACHLIF_002412"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO9606414.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4267..5649)
                     /gene="atpD"
                     /locus_tag="ACHLIF_002413"
     CDS             complement(4267..5649)
                     /gene="atpD"
                     /locus_tag="ACHLIF_002413"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO9606415.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5676..6539)
                     /gene="atpG"
                     /locus_tag="ACHLIF_002414"
     CDS             complement(5676..6539)
                     /gene="atpG"
                     /locus_tag="ACHLIF_002414"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO9606416.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6590..8131)
                     /gene="atpA"
                     /locus_tag="ACHLIF_002415"
     CDS             complement(6590..8131)
                     /gene="atpA"
                     /locus_tag="ACHLIF_002415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO9606417.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLVDVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8144..8677)
                     /gene="atpH"
                     /locus_tag="ACHLIF_002416"
     CDS             complement(8144..8677)
                     /gene="atpH"
                     /locus_tag="ACHLIF_002416"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO9606418.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8692..9162)
                     /gene="atpF"
                     /locus_tag="ACHLIF_002417"
     CDS             complement(8692..9162)
                     /gene="atpF"
                     /locus_tag="ACHLIF_002417"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO9606419.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9224..9463)
                     /gene="atpE"
                     /locus_tag="ACHLIF_002418"
     CDS             complement(9224..9463)
                     /gene="atpE"
                     /locus_tag="ACHLIF_002418"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO9606420.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9510..10325)
                     /gene="atpB"
                     /locus_tag="ACHLIF_002419"
     CDS             complement(9510..10325)
                     /gene="atpB"
                     /locus_tag="ACHLIF_002419"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO9606421.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10334..10714)
                     /gene="atpI"
                     /locus_tag="ACHLIF_002420"
     CDS             complement(10334..10714)
                     /gene="atpI"
                     /locus_tag="ACHLIF_002420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO9606422.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11331..11954)
                     /gene="rsmG"
                     /locus_tag="ACHLIF_002421"
     CDS             complement(11331..11954)
                     /gene="rsmG"
                     /locus_tag="ACHLIF_002421"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO9606423.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12018..13907)
                     /gene="mnmG"
                     /locus_tag="ACHLIF_002422"
     CDS             complement(12018..13907)
                     /gene="mnmG"
                     /locus_tag="ACHLIF_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO9606424.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14285..14728)
                     /gene="mioC"
                     /locus_tag="ACHLIF_002423"
     CDS             complement(14285..14728)
                     /gene="mioC"
                     /locus_tag="ACHLIF_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO9606425.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14818..15276)
                     /gene="asnC"
                     /locus_tag="ACHLIF_002424"
     CDS             complement(14818..15276)
                     /gene="asnC"
                     /locus_tag="ACHLIF_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO9606426.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15428..16420
                     /gene="asnA"
                     /locus_tag="ACHLIF_002425"
     CDS             15428..16420
                     /gene="asnA"
                     /locus_tag="ACHLIF_002425"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO9606427.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16425..17876)
                     /gene="viaA"
                     /locus_tag="ACHLIF_002426"
     CDS             complement(16425..17876)
                     /gene="viaA"
                     /locus_tag="ACHLIF_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO9606428.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17870..19366)
                     /gene="ravA"
                     /locus_tag="ACHLIF_002427"
     CDS             complement(17870..19366)
                     /gene="ravA"
                     /locus_tag="ACHLIF_002427"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO9606429.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19589..21457
                     /gene="kup"
                     /locus_tag="ACHLIF_002428"
     CDS             19589..21457
                     /gene="kup"
                     /locus_tag="ACHLIF_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO9606430.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDTVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21624..22043
                     /gene="rbsD"
                     /locus_tag="ACHLIF_002429"
     CDS             21624..22043
                     /gene="rbsD"
                     /locus_tag="ACHLIF_002429"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO9606431.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22051..23556
                     /gene="rbsA"
                     /locus_tag="ACHLIF_002430"
     CDS             22051..23556
                     /gene="rbsA"
                     /locus_tag="ACHLIF_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO9606432.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23561..24526
                     /gene="rbsC"
                     /locus_tag="ACHLIF_002431"
     CDS             23561..24526
                     /gene="rbsC"
                     /locus_tag="ACHLIF_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO9606433.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24551..25441
                     /gene="rbsB"
                     /locus_tag="ACHLIF_002432"
     CDS             24551..25441
                     /gene="rbsB"
                     /locus_tag="ACHLIF_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO9606434.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25567..26496
                     /gene="rbsK"
                     /locus_tag="ACHLIF_002433"
     CDS             25567..26496
                     /gene="rbsK"
                     /locus_tag="ACHLIF_002433"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO9606435.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26500..27492
                     /gene="rbsR"
                     /locus_tag="ACHLIF_002434"
     CDS             26500..27492
                     /gene="rbsR"
                     /locus_tag="ACHLIF_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO9606436.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27458..28885)
                     /gene="mdtD"
                     /locus_tag="ACHLIF_002435"
     CDS             complement(27458..28885)
                     /gene="mdtD"
                     /locus_tag="ACHLIF_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO9606437.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPVMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28908..29600)
                     /gene="yieP"
                     /locus_tag="ACHLIF_002436"
     CDS             complement(28908..29600)
                     /gene="yieP"
                     /locus_tag="ACHLIF_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO9606438.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7163 a         7615 c         7572 g         7363 t
ORIGIN      
        1 tcagacatta atgataattc agaatatatt tatatagaaa gatagatgaa tttattgatg
       61 tcagttataa aaaagggcca ccggtcggca gccctttttt atctatgata gtgattactc
      121 aaccgtaacc gattttgcca ggttacgcgg ctggtcaacg tcggtgcctt tgatcagcgc
      181 gacatggtaa gccagcagct gcagcggaac ggtgtagaag atcggtgcaa tcacctcttc
      241 cacatgcggc atctcgatga tgtgcatgtt atcgctactt acaaaacccg catcctgatc
      301 ggcgaagaca tacaactgac cgccacgcgc gcgaacttct tcaatgttgg atttcagttt
      361 ttccaacagt tcgttgttcg gtgcaacgac gataaccggc atatccgcgt caatcagcgc
      421 cagcgggccg tgtttcagct cgcctgcagc gtaggcttca gcgtgaatgt aagagatctc
      481 tttcagcttc aatgcgcctt ccagcgcgat tgggtactga tcgccacggc ccaggaacag
      541 cgcgtgatgt ttgtcagaga aatcttctgc cagagcttca atgcgtttgt cctgagacag
      601 catctgctca atacggctcg gcagcgcctg cagaccatgc acaatgtcat gttcaatgga
      661 ggcatccaga cctttcaggc gagacagctt cgccaccagc atcaacagca cagttaactg
      721 agtggtgaat gctttagtgg atgccacgcc gatttccgta cccgcgttgg tcatcagcgc
      781 cagatcggat tcgcgcacca gagaagaacc cggaacgtta cagattgcca gcgaaccaag
      841 gtaacccagc tctttcgaca gacgcaggcc cgccagggta tccgcggttt cgccagactg
      901 tgacaaggtg atcatcaggc tgttacgacg cacggcagat ttgcgatagc ggaattcaga
      961 ggcgatttcg acgtcgcacg gaatacctgc cagcgattca aaccagtaac gggaaaccat
     1021 accggagtta taagaagtac cacaggcgag gatctgaata tgctcaacct tcgacagcag
     1081 ttcgtcggcg ttcggtccca gctcgcttaa atcaacctga ccgtggctga tgcgcccggt
     1141 aagggtgttt ttgatcgcgt tcggctgttc gtagatctct ttctgcatgt agtgacggta
     1201 aataccttta tcgcccgcgt catattgcag attggattcg atatcctgac gttttacttc
     1261 cgcgccagtt ttatcgaaga tgtttaccga acggcgagta atttccgcaa tatcgccctc
     1321 ttcaaggaag ataaagcgac gggtcaccgg cagcagcgcc agctggtcag aagcgataaa
     1381 gttttcgccc atacccagac caatcaccag cggactacca gaacgtgccg ccagcagggt
     1441 atccgggtga cgggagtcca tgatcactgt accgtacgca ccacgcagtt gcgggatagc
     1501 acgcagaaca gcctcacgca gagtaccgcc ctgtttcagc tcccagttca ccagatgggc
     1561 aattacttcg gtgtctgttt cagaaacgaa ggtatagcca cgcgctttta actcttcgcg
     1621 cagcggttca tggttttcga tgatgccgtt atgcaccacc acaatgtgtt cagaaacatg
     1681 cggatgcgca ttcacttctg aaggttcacc gtgggtcgcc cagcgagtat gagcaatacc
     1741 cgtaccgcca tgcagaggat gttcttccgc tgcctgagcc agcatctgga ctttaccgag
     1801 gcgacgcagg cgggtcatat gaccttccgc atcaacaacg gccagaccgg cagagtcata
     1861 tccgcggtat tccagacgac gtaaaccttc aagaaggatt tctgctacat cacgttgcgc
     1921 gatcgcgcca acaattccac acatagtttt tgattccgat ttatatcgtt gtcggtcaac
     1981 ctgtatgccc gttttttcgg gcgccccgag ccttgtagag agtggggtta tttttatagt
     2041 tactgcttgt gggagggaga ttatgttatc tcctcatccc atgtgaccgg attacccggc
     2101 cagaatcact ttttctttac cggacgacgc cagccttctt tctgagtctg cggcacacgg
     2161 ctgatagcta atgcattttc gccgacatta cgcgtcacag ttgtacccgc agcaatggtc
     2221 gcgcctttgc ctactgttac cggggccacc agctgagtgt cggaaccgac aaacacatcg
     2281 tcgccgataa tggtcttaaa tttattcgca ccatcgtagt tgcaggtaat ggttcccgcg
     2341 ccgatgttaa cgttatcgcc aatttccgca tcgcccaggt aagtcagatg accagctttc
     2401 gagcctttac ccagacgcgc ttttttcatc tcaacaaagt taccgacgtg tgcaccttcc
     2461 agcaactcag caccaggacg cagtcgggca aacgggccaa tagtacaggc cgctgccaga
     2521 ttcgcgtctt ccacaacggt atacggactg atttcgcaat catcaccaat cacgctgttt
     2581 ttaatcacgc aaccggtgcc gattttcacg cgatgaccga gagtcacgtt gccctcgatg
     2641 ataacgttag tatcaatttc aacatcgcgc ccgtgagtaa gcgtaccgcg caaatcaaaa
     2701 cgcgccggat cgcgcagcat aacgcctgct aacagcagtt tttcagcctg ttcggactgg
     2761 taaacgcgct ccagacggga gagttgcagg cggttattca caccttctac ttcgcttaaa
     2821 cgttgcggat gaacggcgac gatttcacgc ccttcctgat acgccagcgc aataatgtcg
     2881 gtgatgtagt attcgccctg agcattattg ttggtcagct tcgccagcca gcgtttcata
     2941 tctgcgccgt tagcgatcag aatgccggtg ttgatctcct gaatctgacg ctgctcgtcg
     3001 gtggcatctt tgtgctcaac aatgccggta actttgccat tttcacgggt gatacgtcca
     3061 taaccggtcg gatcatccag tttcaccgtc agcagaccaa tgccaccctg cggtttagca
     3121 tcacgcaggc gctggagtgt ttcgacagag atcagcggca cgtcgccgta gagcattaaa
     3181 atgtcttcat catcggcaaa gaaaggtgcg gcctgctgca ttgcatgacc cgtacccagc
     3241 tgctctgcct gaagcaccca gttaaggttg tcgtctttca gcgcctgttt aagcagatcg
     3301 ccgccgtgac cgtacaccag gtgaacgtgc gctgcgccta attcattcgc agcatcaatg
     3361 acatgctgaa ccatcgcttt cccggcaagg gtatgcagca ctttcggaag atcggaatac
     3421 atgcgcgtgc ctttgcctgc ggcaaggatc actacgctca tagcattatt caacatacgc
     3481 gtcctgactg taatttgaga acgaatttaa accgcttcac cttgaaaaaa ctacattttt
     3541 ttcatcgtga aatggacaga ggataaattg ttcaataacg gattatcccc gctgacgaaa
     3601 gtgccatttt cgaccatcgt cacgcctttt gtctgcagaa aattaaggca gtaccttcat
     3661 ttaagcgtca ggtggatgtt tttgctctta tttcgatcaa tgaataaacg ggaaaacagg
     3721 ggtgttttat atcagcagga tctatgtgaa cgctcttcag gacggatcac gcgtaaaaaa
     3781 aagccagcct gtttccagac tggcttttgt gcttttcaag ccggtgttac atcgcttttt
     3841 tggtcaactc gataacgcgc agctgcgcga tcgctttggc cagttccgca gacgcctgag
     3901 cgtaatctac gtcgccgtga gagctgctaa tgtgctcttc agccttacgt ttcgcttcca
     3961 tggctcgcgc ttcgtcgaga tcctggccgc gaattgcggt gtcggccaga acggtcacgt
     4021 tgccaggctg cacttcaaga atgccgccag acagatagat aaactcttcg tgaccgtgct
     4081 gtttcacgat gcgaatcata ccaggcttaa tggcggtgag cagcggtgcg tggccagggt
     4141 agatccccag ttcaccttcg ctacccgtta cctggatttt ctcgaccaga ccagagaaca
     4201 tttgttgctc tgcgctgacg acgtccaggt ggtaagtcat tgccatatca ccctccgatt
     4261 aaggcgttaa agttttttgg ctttttccac agcttcttcg atggaaccga ccatgtagaa
     4321 cgcctgctcc ggcaggtgat cgtattcgcc ttccatgatg cctttaaagc cacggatggt
     4381 gtctttcagg gagacgtatt tacccggaga accggtgaat acttctgcca cgaagaacgg
     4441 ctgggacagg aagcgctgga tcttacgagc acgcgctacc accagtttgt cttcttcaga
     4501 cagttcatcc atacccagga tggcgatgat gtctttcagt tcctgataac gttgcaggat
     4561 ggactgaacg ccacgcgcgg tgtcgtagtg ttcctgacca accaccagcg ggtccagctg
     4621 acggctggtg gagtccagcg ggtcaacggc cgggtagata cccagagacg cgatctgacg
     4681 gctcagtacc acggttgcgt caaggtgcgc aaaggtggtt gccggagacg gatcagtcaa
     4741 gtcatccgca ggtacgtata ccgcctgtac ggaggtgata gaaccagttt tggtggaggt
     4801 gatacgttcc tgcagaacgc ccatctcttc cgccagggtc ggctgataac ctaccgctga
     4861 aggcatacgg cccagcagtg cggatacttc cgtaccggcc agggtgtaac gatagatgtt
     4921 gtcaacgaac agcagaacgt cacgaccttc gtcacggaat ttctcagcca tggtcagacc
     4981 ggtcagagca acgcgcagac ggtttcccgg cggctcgttc atctggccat acaccaggga
     5041 tactttgtcg ataacgttgg agtcggtcat ttcgtggtag aagtcgttac cctcacgagt
     5101 acgttcacct acgcccgcaa acacagagta accggagtgc tcgatcgcga tgttacgaat
     5161 gagctccatc atgtttacgg ttttacctac acccgcacca ccgaacagac caactttacc
     5221 gcccttagcg aacggacaca tcaggtcgat aactttgata ccggtttcca gcagttcctg
     5281 agagtttgac agctcttcgt aggaaggtgc tgcgcggtga atcgcccaac gctcttcttc
     5341 accgatctcg cctttcatgt cgaccggttc acccagtacg ttcatgatac ggcccagagt
     5401 cgctttacct accgggactt cgatcgggtg ttcgaggtct tttacatcca gaccgcgacg
     5461 cagaccgtcg gaggaaccca ttgcgatggt acgcacgata ccgccgccga gctgctgctg
     5521 aacttccagc accagacgct cattaccatt ttgcacctca agagcatcgt acacgcgcgg
     5581 tacggcatcc tgagggaatt cgacgtcaac tacggcgccg attacctgga caatctttcc
     5641 agtagccatc ttaaatcctc tacgaaataa cctgtttaaa ccgcggcggc ccccgagacg
     5701 atctcggtga gttcctgagt aatgctggcc tgacgagctt tgttgtatac caactgcagc
     5761 tctttaatca ggctgccgcc attgtcggtc gcggctttca tcgccaccat acgggcggcc
     5821 tgctcgctgg ccaggttttc aaccacgccc tgataaacct gagattcgac ataacgacgc
     5881 agcagggtat ccagcaacgc cttcggatcg ggttcgtaca ggtaatccca ggatttatgt
     5941 ttcagatcat catcatctga tgccggtaac ggcagcagct ggctgatggt cggaacctga
     6001 gacatggtgt taataaattt gttgctgaca atgtaaagct tgtccagacg gccttcgtcg
     6061 taggcctgca acatcacttt taccggaccg atcagttcgg acagggaagg gttatccccc
     6121 atgccggtga cctgggcaac aacattgccg cccacggagt tgaagaacga cacgcctttc
     6181 gagccgatca ttgcgaggtc gcattgaacg cctttgtcgg tccaggtctt catttccgcc
     6241 agcagttttt tgaacaggtt aatgttcaaa ccaccgcaca aaccacggtc ggtcgacacc
     6301 accaggtagc ccacgcgttt aacgtcgcgt tcttccaggt atgggtgctt atattccaga
     6361 ttaccgctcg caaggtgacc aatcactttg cgcatggtat ctgcataagg acggctggcc
     6421 gccatgcgat cctgcgattt acgcattttg gaagcggcga ccatctccat cgctttagtg
     6481 atcttttgcg tgttctggac gcttgcgatc ttactacgta tctcttttgc gccggccatg
     6541 agcttctcct caatgccttg cggcctgccc taaggcaagc cgccagacgt taccaggatt
     6601 gggttgcttt gaaggaatcg aggatgcctt tcagcttgcc ttcgatttcg tcgttgtagc
     6661 caccggtctg gttgatctct tgcatcaacg gagcgtgatc acggtcgacg taagccagca
     6721 gagcggcttc gaagctgccg attttcgaca gttcaacatc caccaggtaa ccacgttctg
     6781 ctgcgaacag aaccagagac tgttgcgcta cggacatcgg cgcatactgt ttctgtttca
     6841 gcagttcggt cactttctga ccgtggtcaa gctgcttacg tgttgcatcg tcaaggtcgg
     6901 atgcaaactg agagaacgct gccagttcac gatactgtgc cagagcggta cggataccac
     6961 cggacagttt tttcatgatc ttggtctgtg ctgcaccacc aacacgggat acggaaatac
     7021 ccgggttaac cgcaggacga ataccggcgt tgaacaggtt ggtttccagg aagatctgac
     7081 catcggtaat ggagattacg ttggtcggaa cgaacgcaga aacgtcaccc gcctgagttt
     7141 caataatcgg cagtgcggtc agagaaccgg ttttcccttt cacttcacct ttggtgaagg
     7201 cttcaacgta ttcggcgtta acacgcgcag cacgctccag cagacgagag tggaggtaga
     7261 atacgtcgcc cgggaatgct tcacgtcctg gcggacgacg gagcagcagg gagatctgac
     7321 ggtaagcaac agcctgttta gacaggtcat cgtaaatgat cagcgcatct tcaccgcggt
     7381 cacggaagta ttcgcccatt gcgcaaccgg catacggagc cagatattgc agtgcagcgg
     7441 attcagacgc ggttgctacc acaacgatgg tgttagccag tgcgccgtgc tcttccagtt
     7501 tacgtaccac gttagaaatg gtggacgctt tctggccgat agcgacatag atacatttga
     7561 taccggaatc gcgctggttg atgatggcat cgatagccag tgcggtttta ccggtctgac
     7621 ggtcaccgat gatcaattca cgctgaccac gaccaattgg gatcatggag tcaacggctt
     7681 tataaccggt ctgtaccggc tgatctacgg actgacgttc gataacgccc ggagcgattg
     7741 cttctacagc agagaagccg tcgtgatcca gcggaccttt accgtcgatt ggtgcaccca
     7801 gagtgttaac cacacggccc agcaggccac ggccaaccgg aacttccagg atacggccag
     7861 tacacttaac tttcatgcct tcggcaaggt cagcgtacgg acccataaca accgcaccta
     7921 cagagtcgcg ctcgaggttc agtgcgatag cgtaacggtt acccggcagg gagatcattt
     7981 caccctgcat acaatcggcc aggccgtgaa tgcggataac accgtcactt acagaaacaa
     8041 tagtaccttc gttgtgagct tcactcacaa cattgaactg agcaatgcgc tgcttgatca
     8101 gttcgctgat ttcggtggaa ttcagttgca tgctccagtc cccttaagac tgcaagacgt
     8161 ctgcaaggcg ctcaagacga ccgcgtacgc tgccatcaat gaccatatca cccgctcgga
     8221 tgataacgcc tgccattaca gacttatcga ttttgcaatt cagcttaact ttgcgtgaca
     8281 gacgtttttc catcgcagca gaaattttcg cgagctgttg ttcactcagt gcggcagcgg
     8341 aaatgacgtc tacctcagcg gtagcctcac tcacggcacg aaggtgaata aactgctcca
     8401 gaacatccgg gagcgcgtta agacgaccat tttcagccat tacccgaatc aggttctgac
     8461 cgttttcgtc cagttgctca ccacaaactg cgataaacga ctcggcgagc gtttctggcg
     8521 caagcgcgcc agagagaagc tctgccattt gttcgttttt ggttacctcg gcggcaaacg
     8581 ccagcatgtc ctgccagcgt tctacacttt ggtgttcgac ggcaaagtca aaagctgctt
     8641 tggcgtaggg gcgagctacc gtaataaatt cagacatcag cccctccctc cttacagttc
     8701 agcgacaagt ttatccacga tgtcgctgtt agcagcttca tccacggaac gttcgatgat
     8761 cttctcggcg ccagcaacag ccaggatagc aacttgctta cgcagctctt cacgggcacg
     8821 tttacgctcg gcttcaattt ctgcctgcgc ctgggccacg attttagtac gttcctgttc
     8881 tgcctcagct ttcgcttcgt ccagaatctg cgagcggcgt ttgttcgcct gctcgatgat
     8941 tacctgggct tccgctttcg cttttttcag ctggtcggtc gcgctggcct ttgcaaggtc
     9001 aaggtcctta tgtgctcgtt ctgcggaagc aaggccgtca gcaatttctt tttgacgttt
     9061 ttcgatggct gccattaatg gcggccatac gtacttcatg cagaacagaa cgaacaggac
     9121 aaacgcgatg gcctggccga ggattgttgc gttaagattc acagcacaat gcctctattt
     9181 agttaacgtt ctgatattgc tctttaaata aaagcaacgc ttactacgcg acagcgaaca
     9241 tcacgtacag acccagacct acagcgatca tcgggatagc atccaccaga cccataacga
     9301 taaagaactg agtacgcagc agaggaatca gatcaggttg acgcgctgcg ccttccagga
     9361 atttaccccc gaggatgccg ataccgatcg cagcaccgat tgccgccaga cccatcatca
     9421 cagcggcagc catgtacagc agatccatat tcaggttttc catgacagtc tccagtttgt
     9481 ttcagttaaa acgtagtagt gttggtaaat taatgttctt cagacgccat cgacagatag
     9541 acgatcgtca gaaccatgaa gatgaaggct tgcagcgtaa tgatcaggat gtggaaaatg
     9601 gcccacggca cattcaggat ccactgtgac caccacggca acagaccagc aatcagaatg
     9661 aaaatcagct caccggcata catgttaccg aacagtcgca aaccaagtga aactggtttg
     9721 gacagcaggc ttaccccttc aaggattaag ttgacaggaa tgaacgccca gtgattgaac
     9781 ggctgcagcg tcaactcttt cgtgaagccg ccgatgcctt tcattttgat gctgtagaac
     9841 agaatcagga taaatacgcc caatgccata gacagcgtta cgttcacgtc cgcagacgga
     9901 accacacgca gtgcaggcag acccagtaca tgttcagcaa tgtacggcag caggtcgata
     9961 ggcagtaaat ccatcaggtt catcaggaat acccagacga agatcgtcag ggccagcgga
    10021 gcaatcagct tgcttttgcc atggtacatg tctttcacgc taccattaac aaagccgatc
    10081 accagctcaa tcgcggtctg aaacttacct ggcacaccgc tggtcgcctt tttggctacg
    10141 ctacggaata aaaccaggaa caacagaccc agcaccaccg agaagaacat ggagtcaata
    10201 ttgattgtcc agaaggtggc tggggggttt tgtggatcca ccagcgagaa tgtacgcagg
    10261 tccagctgaa ggttattcag gtggtgtcct atgtaatcct gcggcgtcat attttctgaa
    10321 gccatgatgc cttttaccct ttgttgttaa ttacagccgg tgccagtatc tgaaccacca
    10381 gcaccaaaac ccacgtaacg atcagcggca agaataccgc ctttaaaacc gccaacgcca
    10441 ccaccagtaa caccaacatc gccagaactt tgaaagcttc gccaaatgcg aatgtccagg
    10501 ccacccggcc tttcgctggt gtatgcgcct ggtgacgcca ggcaaatatc ataaacaaaa
    10561 cgttaggcag aaagactgcc aggcccccgc ttattgcaga gacgccccag aaggggtctt
    10621 tgaggctgaa cagcaatcca cttgctatca ccaccagtaa ctgaacgagc agaagcttcc
    10681 gagcaacgtt tcgactcacg agcgacacag acatcacgtt tttcactcct gctcccttcg
    10741 aggtatgccg cgtgtcgtat aaaactttct ttaaggctta gagtcaagca tcaaaaagcg
    10801 gtcaaattat acggtgcgcc cccgtgattt caaacaataa gtagccaaaa ggtgaataaa
    10861 tgtttaaata tttttccagt gcatacaatt gcgacttttc tgctaaccct gttcgatcat
    10921 gaaaaactgt aaataacgcg taaacactgg tgataaagcg tgcttcagat cacatattgc
    10981 gcatgttcgc gcacagcata tttatttact tggcaaatga tgcctttgca agtttatgat
    11041 atttcagtct aaaaacagat actgttttaa taaatgacat ttacacaaca aaaaccaccc
    11101 attgacattt ttaataatgt tttaacagcc aatgatggtt cttagcgccg atttttagca
    11161 gactgatatt ttcactaatg acttattttc tgcttaccaa aaaaagccac gttatcttgt
    11221 tgatgcaaaa gagtgaacgt ggcgttaaat gtaaccagtt atatcagtag aaaacctggt
    11281 tgttgttaac agtctaaccg gtcaattttt tatgattttt ttgataaaaa ttaaatttta
    11341 tttgctttaa tcaccaccag atgacgttcg ccatccaagg ctggaacctg aagtttaacc
    11401 actgattcga cctgatattc ttcgggcaac aaagcgattt catcttccgg catttgccct
    11461 ttcagcgcgt agaaacggcc ttgctcacca ggaagatggt ggcaccagct caccatatcg
    11521 ttcagagagg caaaagcgcg gctaattacg ccatcaaatg gcggctctga aggaaactct
    11581 tctaccctgc tctgtactgg ttcaatattc tccagtttaa gctcatgttg cacctgacga
    11641 aggaaacgca cgcgtttacc aaggctatcc aacagagtga aatgggcttc aggacgcacg
    11701 atagagagtg gaatgcctgg cagtcctggt ccggtgccga catcgataaa ccgttcacct
    11761 tgcagatacg gtgccaccac aatgctatcg agaatatggc gtaccagcat ctcattagga
    11821 tcgcggaccg aagtcaggtt gtacgctttg ttccatttat gcagcatatt cacgtaggca
    11881 ataagctggt ttttctggtg atcggtaagc gaaatacctg cgtctttcag cagtaaggag
    11941 agtttgttga gcacggtgat tacctgttct tgatgcgttg cctggtaagc gggtgcttac
    12001 caggcatttt taatgcgtta tgcgctacga cgcagcatac cctgtttttt cagccacacc
    12061 agcagaatgg agatggccgc aggcgtgacg ccagaaatac gcgaagcctg gccgatagag
    12121 gctggtttgt gatcgttaag tttggcgatc acttcgttag aaagaccgga tacctggcgg
    12181 taatccagtg tcgcgggtag cagggtgttc tcgttacgca gctgcttttc gatctcatct
    12241 tgctggcgcg cgatataacc ttcgtattta acctgaatct caacctgttc cgccgcctgt
    12301 tcgtctgtca acgcaggggc aaacggcgtc agcgtggtta atttttcata agtcatttcc
    12361 ggacgacgca gcagatcttc accactggct tcacgggaaa gcggcgcagt caggtgagca
    12421 ttcacttcgg ctgcagcttc cgccgacggg gttacccagg tcgatttcag acgctgacgc
    12481 tcacgctcga tattctcaag tttctcgtta aagcgcgccc aacgttcgtc atccaccagg
    12541 cccagttcac gaccgatttc agtcaaacgc agatccgcat tatcttcgcg tagcatcaga
    12601 cgatattctg cgcgcgaagt aaacatacga tacggttctt tggttcctaa agtgcacagg
    12661 tcatcaacta gtacgccgag atacgcctga gaacgtgccg gagcccaacc ttctttgtca
    12721 gcagacagac gggcagcgtt aagaccggcc agcaaacctt gcgcagcggc ttcttcgtaa
    12781 ccggtagtgc cgttaatctg accagcaaag aacagcccct ggataaactt gctctccagc
    12841 gtcggtttca ggtcgcgagg atcgaagaag tcatactcaa tggcataacc cggacgcacg
    12901 atcttcgcgt tttccatccc ctgcatagag cggacgattt gcatctgcac atcgaacggc
    12961 aggctggtgg agataccgtt cggataaatt tcattagagg tcagtccttc cggttcaagg
    13021 aagatctgat gctgatttct gtcggcgaag cgcataactt tgtcttcgat cgacgggcag
    13081 tagcgtgggc cgacaccttc gatcacccct gcatacattg ggctacgatc gaggttactg
    13141 cggatcacat catgggtttt ctcgttggta tgagtgatat aacacggcac ctgctgggga
    13201 tgctgggacg cattgcccat aaacgagaat accggcattg ggttatcgcc atgctgttgc
    13261 gccagcacac taaagtcgat agttcgagca tcaatacgcg gtggtgtccc ggttttcaga
    13321 cgaccaacgc gcagcggcag ttcacgcaaa cggcgagaaa gcggaatgga cggcggatca
    13381 ccagcacggc caccgctgta attatccaga ccgatatgaa ttttaccgtc gaggaacgtc
    13441 ccaacggtga gcacgacggc tttggcacgg aacttcagtc ccatttgggt aacagcaccg
    13501 accacgcgat cgttttcgac aataagatct tcaaccgcct gctggaagat catcaggttc
    13561 ggttggttct ccagcgccgt acgtaccgcc tgacggtaga gcacacgatc cgcctgagct
    13621 cgggtagcgc gaaccgccgg tcctttgctt gcgtttagta tcctaaactg gatacccgct
    13681 tgatcgatcg ctttcgccat cagaccgccg agtgcatcca cttcttttac cagatgtccc
    13741 ttcccaatac cgccgatcgc cgggttgcag ctcatctgcc ccagagtgtc gatattgtgt
    13801 gtcaaaagca gagtctgttg acccatacgc gccgcggcca tcgcggcctc ggtgcctgca
    13861 tgacccccgc caatgatgat gacgtcaaaa ggatccggat aaaacatggt gattgcctcg
    13921 cataacgcgg tatgaaaatg gattgaagcc cgggccgtgg attctactca actttgtcgg
    13981 cttgagaaag acctgggatc ctgggtatta aaaagaagat ctatttattt agagatctgt
    14041 tctattgtga tctcttatta ggatcgcact ccctgtggat aacaaggatc cggcttttaa
    14101 gatcaacaac ctggaaagga tcattaactg tgaatgatcg gtgatcctgg accgtataag
    14161 ctgggatcag aatgaggggt tatacacaac tcaaaaactg aacaaccgtt gttccttgga
    14221 taactaccgg ttgatccaag cttcctgaca gagttatcca cagtagatcg cacgatctgt
    14281 atacttattt gagtaaatta atccacgatc ccagccattc ttctgccgga tcttccggaa
    14341 tgtcgtgatc aagaatgttg atcttcagtg tttcgcctgt ctgttttgca ccggaatttt
    14401 tgagttctgc ctcgagttta tcgatagccc cacaaaaggt gtcatattca cgactaccaa
    14461 taccgattgc gccaaagcgg accgcagaaa gatcgggctt ctgttcctgc aatgcttcat
    14521 agaaaggaga aaggttgtcc ggaatatctc cggcaccgtg ggtggagctg ataatcagcc
    14581 agatccccga ggcagataaa tcttccaaca gcggaccgtg cagcgtttcg gtggtaaaac
    14641 ccgcctcttc cagcttttca gccaggtgtt ctgctacata ttcggcaccg ccgagggtgc
    14701 tgccgctgat aagagtgata tctgccataa accgccacct ttattaagag tggcgtattg
    14761 tacgctgtga acgcgttggg atctacctgt ggaaaagtat gggattaaaa aagccgatta
    14821 tggcttgatg gtacgcatga tcgggttttg caggacgatc aatgtctcgg tggactgaat
    14881 ttcatcaatt gtttggatct tgttgataag tacatgctgg agagcgtcga tcgaacggca
    14941 catcactttt ataaagatgc tgtagtggcc ggttgtgtaa taggcttcag tgacttcatc
    15001 aaggctttcc agctttgcca gcgcggaagg gtagtctttg gcgctcttta atataatgcc
    15061 gataaagcag cctacgtcat aaccgagctg cttcgggctg acatcaatac gcgccccggt
    15121 aatgatcccc gcctgcttca ttttctctac tcgaacgtga atcgtccccg gactgacgcc
    15181 aaattgtttc gccagttcgg cgtaagcggt gcgcgcattg cccattaatg cttccaggat
    15241 gccacggtcc agattgtcga tcagataatt ttccatagga ttttcttatg cagattgatg
    15301 attcattcta ttttagcctt cttttttaat gaatcaaaag tgtgttaggc tttttattga
    15361 atgattattg catgtgtgtc ggtttttgtt gcttaatcat aagcaacagg acgcaggagt
    15421 ataaaaaatg aaaaccgctt acattgccaa acaacgccaa attagcttcg tgaaatctca
    15481 cttttctcgc caactggaag aacgtctggg gctgatcgaa gtccaggcgc cgattcttag
    15541 ccgtgtgggg gatggcacgc aggataactt gtcgggctgt gaaaaagcgg tgcaggtaaa
    15601 agtgaaagct ctgcctgatg cccagttcga agtggttcat tcactggcga agtggaaacg
    15661 tcagacctta gggcaacacg acttcagcgc gggcgaaggg ctgtacacgc acatgaaagc
    15721 ccttcgcccc gatgaagagc gtctttctcc gttgcactcg gtctatgttg accagtggga
    15781 ctgggaacgc gtaatgggcg acggtgagcg tcaattctcg actctgaaaa gcacggtaga
    15841 ggcgatctgg gcgggaatta aagcaaccga agctgcagtt agcgaagagt ttggtttggc
    15901 accgttcctg ccggatcaga tccacttcgt acacagccag gagttactgt ctcgttatcc
    15961 ggatctcgat gccaaagggc gtgagcgggc gattgcgaaa gatcttggcg cggtattcct
    16021 cgtcgggatt ggcggcaagc tgagtgatgg tcatcgtcac gacgtgcgcg caccggatta
    16081 tgatgactgg agcaccccgt cagagctggg ccatgcgggt ctgaacggcg atattctggt
    16141 atggaaccct gtactggaag atgcgtttga gctttcttcc atggggatcc gcgtggatgc
    16201 cgacacgttg aagcatcagt tggcgctgac cggtgatgaa gatcgtctgc agctggaatg
    16261 gcatcaggcg ctgctgcgcg gtgaaatgcc gcagaccatc ggcggcggta tcggccagtc
    16321 tcgtttgacc atgctgctgc tgcaactgcc gcatatcggc caggttcagt gtggagtatg
    16381 gccagctgct gttcgcgaga gcgtcccttc tctgctgtaa taatttatcg ccgccagcgt
    16441 ctgagcaggc ggcttcgcat cccggtatca aagcgccaga tatgatcgaa aatgcgcatg
    16501 atgccgggtt tgccgtgtgc cgacatcgcc acggcatgaa agcgatgctg atgtacccgc
    16561 tgcagctctt tcactttact cgtcacgtcg tcaggcaacc gctgagcgat aaaatcagaa
    16621 atcaccaccg catcggcatc aaaccattcc ctgctttgca agcgttccat aatggcgcga
    16681 aaacaactgg caagatcggt gccgccacga aactgctggc ttaaaaaacg gattgcttgt
    16741 tcgatgcctt gtgggcctga aagctcataa cggacgatct cggtggaaaa tagcataata
    16801 tagcagcgcc ggttttcagc gagagcaatg cgcatcaagg ccaggcagaa cgctttcgca
    16861 cactgttcat taaagccgcc cattgagccg gaagtatcca cacagacaat aaacggcccg
    16921 cgcggctgtt catcgtaatc tttatgtacc accggacgtt cgatcacttt ttcacgccac
    16981 gactcaccgt gcaggcgata ggtgagcaac tgtttttcca ccagccgacg gtaaaactca
    17041 tactccagtt ccgttatccc tagtgtcgcc agttctggcg gcagaagacg taaaatatca
    17101 tcgctttgtt gcagaccatc aacctgctca ggaaccgtcg ccggttcgcg caccatggtg
    17161 cggaaggttt ccatctgcgc atcgttgcgc ggtattgatt tggcttcccg agaacgcccc
    17221 agctgctctg ccaggcgttt cagttccggc tgttcgttaa gaaattcacc gtatttcaca
    17281 atcaactgat agtcgccacg tttaagctga ccggcgctca tatcccacag acgaccagct
    17341 gcagtattgt tatctgcgag aatcggttca agttgtccgc tcagcgtcat gcgttcctga
    17401 acttcactca acagttgttc gcgttcttct tctaatagct gttgatttaa cgtcgttgct
    17461 tgcacgatca gacttaaacg ccaacgctgg agaaaaagcg tgtgtaacgc tgaagtgatc
    17521 gtgctgttag catcaaccaa ctgtcgggct tgttctgccc atggagaatt cagacgatgc
    17581 agtaagtcca ggatctgtgg tagctgcaca ataaactgtg gcgtggagag gagctggctt
    17641 tgctgatagc acatcacctc ttcggtgagt tccggcggga ctcgggcatc tttcagccga
    17701 ctgcgcagcg cttcacgcca gcggggaaca tcatcagtga ttgccgcctt caggcgtggg
    17761 aatttttcaa agaagaccgc cagctgcggt gaggccagca gtgcgatgat catctcttcg
    17821 atcaatccct cttcgctgac ggccagcatc acattaagcg tatccagcgt tagcattgtt
    17881 gtgcctggcg aatctgtgca ccgacatcct gtaggctggc ttcaatgcga cctaaccagt
    17941 cgccagggat aaacagacat ttttgctgtt cgctgaataa agcgtgttgc ttgcgccagt
    18001 cgctttcaag ctcttccagt tgttgtttta tttcaccagg cagaccttca gccgatgaac
    18061 cgggaagtgc cagcgtactg ccttgtaaac ttacatcgcg aacgacaaga tgttgggcgc
    18121 tatcaacttc cagattcagt ttctgggcaa agccgatacc gttcagttta ccgcgaattt
    18181 caccaccttt gctcagccac tgttccagcg cgctacgctc aaaggagata tgaaccactt
    18241 ccatatcatg cagttttaac ggtttttgca gcagtagagt cagagtggaa gcagtaacgt
    18301 taacaggaag ttgatactgc tgacgacggc tgaaaatgcc gcccagacga attaccgtta
    18361 aggctgtttt atcgctttgt tgctgctgga gttgcaggtg acgttgcaca atcgcgccaa
    18421 ggcgagtcaa catcccttgc tgttgccagg cgtgaccggt catcaataca tcaatttgtt
    18481 gttgtatcaa attcaggctt tgcgcgtcat accacaggca atctttcagc aaaatgagat
    18541 ctaccggggc aacagcactg cgaccgctaa aaaaggcgct ggcctgcaat aatcggatcg
    18601 cttttttcca gcgacgatcc gagacataag gcgcatccgg taatttatcc agttgctggc
    18661 gcagcataaa aatcagctca aatacatgat cgggcagcgt aatttcacca atctctttct
    18721 gccagcgttc atattcttca tctgtgacct gcaaggcatc aggaaccgga ttgtcgtttt
    18781 catcctgttg actggtcagc atggagcgga aattcgcttt atcctgcact ttatctaacc
    18841 acagacgaat cagcatgcgg tcatataacg cttccagact gctgtctgct tccggcagct
    18901 cgttggaggc cgccaccagc agacgcatcg ggattttttc tacgtgtgca ccgttgcgga
    18961 actggcgctc gttaatggcg gtgagcaagg tattaagaat tgccgggccc gctttccaga
    19021 tctcatccag aaagacaatt tctgcttccg gcaggtaacc gctggttaaa cgttcatagc
    19081 gcccttcatc ttttagcgcc tgaatagaaa ggggaccaaa aacttcttcc ggcgtggaga
    19141 agcgggtcat cagatattca aacgcgcggg cattctgaaa ggcgaatttt aagcgccggg
    19201 cgatcaaact tttggcaata cctggcgggc caaggaggaa cacactttca ccacttaatg
    19261 ccgctaataa acacaagcgg atggcgtggc tacgttcata aagccccttt tccaacgaac
    19321 tgctcaggcg ggaaattctt tccgctaata aatgagggtg agccataatg aagtggcgtc
    19381 ctttcgtcaa aagttctgcg taaattgcga gtatagacgt ttcttgctgg tggctaaaat
    19441 agtctcaaag ggggggtatt tttctttgag ccaggttaat gtggccgcat ttaggagtac
    19501 gattttgccg ttaatcgtgc atactgtgcg cttttttgtg ggccaaggga ctaagcacac
    19561 atttcatatt tcaacgaaag actagtctat gagcactgat aataagcaat cattgcccgc
    19621 gattaccctc gcggcgattg gagttgtcta cggcgatatt ggtaccagcc cgttatatac
    19681 acttcgtgaa tgtttgtccg gccagtttgg ttttggcgtt gaacgcgata ccgtgtttgg
    19741 ctttttatcg ctgatcttct ggctgctaat ctttgtggtt tccattaaat atctcacctt
    19801 cgtgatgcgg gcagataacg ccggtgaggg ggggatcctg acgttgatgt cgcttgccgg
    19861 gcgtaatacg tcggcgcgaa ccacatcaat gctggtgatt atggggctaa tcggcggcag
    19921 ctttttctat ggtgaagtcg tcataacacc cgctatttcg gtgatgtcag ccattgaagg
    19981 tctggaaatc gtcgccccgc agctggatac ctggatagtt cccctctcaa ttatcgttct
    20041 cactttatta tttatgattc aaaaacatgg cacggctatg gtcggtaagc tgtttgcgcc
    20101 gatcatgctg acctggtttt tgattctggc aggactgggg ttacgtagca ttattgctaa
    20161 cccggaagtg ctgcatgcgc tgaatccaat gtgggcggtg catttcttcc ttgaatacaa
    20221 aacggtttct tttattgcat taggggcagt ggtgttgtcg attacggggg tcgaggcgct
    20281 gtatgctgat atggggcact ttggtaagtt ccctattcgc ctggcgtggt tcaccgtcgt
    20341 attaccttcc ttgaccctta attacttcgg tcagggagcg ctgttgttaa agaacccgga
    20401 agcgattaag aacccgttct tcctgttggc accggactgg gcgctgatcc cgctgctgat
    20461 catcgccgca ctggcgacgg taattgcctc gcaggcggtt atctctggcg tcttctcatt
    20521 gacgcgtcag gcggtacgtc tgggatattt gtcgccgatg cgcattattc acacctccga
    20581 aatggagtca gggcaaatct atattccgtt tgtgaactgg atgctctatg tcgcggtcgt
    20641 gattgtgatt gtcagctttg agcactccag caacctggcg gcggcgtacg ggattgcggt
    20701 gaccggaacc atggtgctga cgtctattct ctcgactacc gtggcacgtc agaactggca
    20761 ctggaataag tattttgttg cgctgatcct gattgctttc ctttgtgtcg atattccatt
    20821 gttcaccgct aacctcgata aactgctctc cggcggctgg ttgccattga gcctcggtac
    20881 tgtgatgttt atcgtgatga ccacctggaa gagcgagcgt ttccgcttgc tgcggcggat
    20941 gcatgaacat ggtaactctc tggaagcgat gattgcttcg ctggagaaat caccgcctgt
    21001 tcgcgtgccc gggaccgcgg tgtatatgtc gcgtgcaatc aacgtcattc cctttgcgct
    21061 gatgcataac cttaaacata acaaggtatt gcatgagcgg gtgattctgt taactctgcg
    21121 taccgaagac gcgccatatg tccataatgt ccgtcgggta cagattgaac aactgtcgcc
    21181 cactttctgg cgcgtggtgg caagttatgg ttggcgagaa acgccaaacg tggaagaagt
    21241 tttccaccgc tgcggtctgg aaggattaag ttgccggatg atggaaacct ccttctttat
    21301 gtcgcatgag tcgttgatcc tcggcaaacg cccgtggtat ttgcgtctgc gcggcaagct
    21361 gtacttgctg ctgcaacgta atgcgctgcg tgcaccagat caatttgaaa ttccgccaaa
    21421 cagggttatc gaactgggta ctcaggtcga aatctaacgc cagacgcctc ctttctccat
    21481 aagggggcgt ttttgttttc atggttaatc accatgtaaa acgtttcgag gttgatcaca
    21541 tttccgtaac gtcacgatgg ttttcccaac tcagtcagga ttaaactgcg ggtcagcgaa
    21601 acgtttcgct gatggagaaa aaaatgaaaa aaggcaccgt tcttaattct gatatttcat
    21661 cggtgatctc ccgtctggga cataccgata cgctggtggt gtgtgatgct ggtttaccta
    21721 tccccaaaag tacaacgcgt atcgatatgg cattaaccca gggtgtacct tcttttatgc
    21781 aggtgctggg cgtcgtcaca aatgaaatgc aggtcgaggc tgccattatc gcggaagaga
    21841 tcaaacaaca taatccgcaa ctccacgaaa cgttgctcac tcaccttgag cagctgcaaa
    21901 aacaccaggg aaataccatt gaaattcgtt acaccacgca tgaacaattc aaacaacaaa
    21961 ccgcagaaag tcaggcggta attcgcagcg gagaatgttc tccgtatgcg aatatcattc
    22021 tctgtgctgg cgtgacgttc tgaggccgtc atggaagcat tacttcagct taaaggcatc
    22081 gataaagcct tcccgggcgt aaaagccctc tcgggcgcag cgttaaatgt ctatccgggc
    22141 cgcgtgatgg cgctggtggg cgaaaacggc gcgggtaaat ccaccatgat gaaagtgctt
    22201 actggtatct atgctcgcga tgccggcacg cttttatggc tggggaaaga aacgacattt
    22261 accgggccga aatcttccca ggaagccggg attgggatta tccatcagga actgaacctg
    22321 atcccgcagt tgaccattgc cgaaaacatt ttcctcggtc gtgagtttgt taatcgcttt
    22381 ggcaaaattg actggaaaac catgtatgcc gaagcggata aattgctggc taaacttaac
    22441 ctgcgcttta aaagcgacaa gctggtgggc gatctttcca tcggtgacca gcaaatggtt
    22501 gaaatcgcca aagtgctgag ctttgagtcg aaagtcatca ttatggatga accgaccgat
    22561 gcgctgaccg ataccgaaac cgaatccctg ttccgcgtca tccgcgaact aaaatcgcaa
    22621 ggccgcggta ttgtctatat ctcccaccgc atgaaagaaa tcttcgagat ttgcgatgac
    22681 gttaccgttt ttcgtgacgg gcaatttatt gctgagcgcg aagtggcatc actgaccgaa
    22741 gattcgctga ttgagatgat ggtgggccgc aagctggaag atcaatatcc acacctggac
    22801 aaagcgccgg gagatatccg cctgaaagtc gataatctct gcggacctgg cgttaacgat
    22861 gtctctttta ctttacgcaa aggcgaaatt cttggcgtct ctggtttgat gggcgcaggt
    22921 cgtaccgaac tgatgaaagt gctctacggc gcactaccgc gcaccagcgg ttacgtcacc
    22981 ctggatgggc atgaagtcgt tacccgttca ccgcaggatg gcctggcaaa cggcattgtg
    23041 tatatctccg aagaccgtaa acgtgacggt ttagtgttgg gcatgtcagt aaaagagaac
    23101 atgtcgctga cagcgctgcg ctacttcagc cgcgctggcg gcagtttgaa gcatgccgat
    23161 gaacagcagg ctgtgagtga tttcattcgt ctgtttaatg tgaaaacacc gtcgatggaa
    23221 caggcaattg gtctgctttc cggtggcaat cagcaaaaag tggcgattgc ccgcggtctg
    23281 atgacacgcc ccaaagtgtt gatccttgat gaacctaccc gtggcgtaga tgtcggcgcg
    23341 aaaaaagaga tctatcaact gattaaccag ttcaaagccg atggcttgag catcattctg
    23401 gtgtcatcgg agatgccaga agtattaggc atgagcgatc gcatcatcgt catgcatgaa
    23461 gggcatctca gcggggaatt tactcgtgag caggccaccc aggaagtgtt aatggctgcc
    23521 gctgtgggca agcttaatcg cgtgaatcag gagtaaaaaa atgacaaccc agactgtctc
    23581 tggtcgccgt tatttcacga aagcgtggct gatggagcag aaatcgctta tcgctctgct
    23641 ggtgctgatc gcgattgtct cgacgttaag cccgaacttt ttcaccatca ataacttatt
    23701 caatattctc cagcaaacct cggtgaacgc cattatggcg gtcgggatga cgctggtgat
    23761 cctgacgtcg ggcatcgact tgtctgtagg ttctctgttg gcgctaaccg gcgcagttgc
    23821 tgcatctatc gtcggcattg aagtcaatgc gctggtggct gtcgctgccg ctctcgcgtt
    23881 aggtgccgca attggtgcgg taaccggggt gattgtagcg aaaggtcgcg ttcaggcgtt
    23941 tatcgctacg ctggttatga tgcttttact gcgcggcgtg accatggttt ataccaacgg
    24001 tagcccagtg aataccggct ttactgagaa cgccgatctg tttggctggt ttggtattgg
    24061 tcgtccgctg ggcgtaccga cgccagtctg gatcatgggg attgtcttcc tcgcggcctg
    24121 gtacatgctg catcacacgc gtctggggcg ttacatctac gcgctgggcg gcaacgaagc
    24181 ggcaacgcgt ctttctggta tcaacgtcaa taaaatcaaa atcatcgtct attctctttg
    24241 tggtctgctg gcatcgctgg ccgggatcat tgaagtggcg cgtctctcct ccgcacaacc
    24301 cacggcgggg actggctatg agctggatgc tattgctgcg gtggttctgg gcggtacgag
    24361 tctggcgggc ggaaaaggtc gcattgttgg gacgttgatc ggcgcattaa ttcttggctt
    24421 ccttaataat ggattgaatt tgttaggtgt ttcctcctat taccagatga tcgtcaaagc
    24481 ggtggtgatt ttgctggcgg tgctggtaga caacaaaaag cagtaataac gactacagga
    24541 catcttgaat atgaacatga aaaaactggc taccctggtt tccgctgttg cgctaagcgc
    24601 caccgtcagt gcgaatgcga tggcaaaaga caccatcgcg ctggtggtct ccacgcttaa
    24661 caacccgttc tttgtatcgc tgaaagatgg cgcgcagaaa gaggcggata agcttggcta
    24721 taacctggtg gtgctggact cccagaacaa cccggcgaaa gagctggcga acgtgcagga
    24781 cttaaccgtt cgcggcacca aaattctgct gattaacccg accgactccg acgcagtagg
    24841 taatgctgtg aagatggcta accaggcgaa catcccggtt atcactcttg accgccaggc
    24901 aacgaaaggt gaagtggtga gccacattgc ttctgataac gtactgggtg gcaaaatcgc
    24961 cggtgattac atcgcgaaga aagcgggtga aggtgccaaa gttatcgagc tgcaaggcat
    25021 tgctggtaca tccgcagccc gtgaacgtgg cgaaggcttc cagcaggccg ttgctgctca
    25081 caagtttaat gttcttgcca gccagccagc agattttgat cgcactaaag gtttgaacgt
    25141 aatgcagaac ctgttgaccg ctcatccgga tgttcaggct gtattcgcgc agaatgatga
    25201 aatggcgctg ggcgcgctgc gcgcactgca aactgccggt aaatcggatg tgatggtcgt
    25261 cggatttgac ggtacaccgg atggcgaaaa agcggtgaat gatggcaaac tagcagcgac
    25321 tatcgctcag ctacccgatc agattggcgc gaaaggcgtc gaaaccgcag ataaagtgct
    25381 gaaaggcgag aaagttcagg ctaagtatcc ggttgatctg aaactggttg ttaagcagta
    25441 gttttaatca ggttgtatga cctgatggtg acataaatac gtcatcgaca gatgaacgtg
    25501 taatataaag aaaagcaggg cacgcgccac cctaacacgg tggcgcattt tatggacatc
    25561 ccgaatatgc aaaacgcagg cagcctcgtt gttcttggca gcattaatgc tgaccacatt
    25621 cttaatcttc aatcttttcc tactccaggc gaaaccgtaa ccggtaacca ctatcaggtt
    25681 gcatttggcg gcaaaggcgc gaatcaggct gtggctgctg ggcgtagcgg tgcgaatatc
    25741 gcgtttattg cctgtacggg tgatgacagc attggtgaga gcgttcgcca gcagctcgcc
    25801 actgataaca ttgatattac tccggtcagc gtgatcaaag gcgaatcaac aggtgtggcg
    25861 ctgatttttg ttaatggcga aggtgagaat gtcatcggta ttcatgccgg cgctaatgct
    25921 gccctttccc cggcgctggt ggaagcgcaa cgtgagcgta ttgccaacgc gtcagcatta
    25981 ttaatgcagc tggaatcacc actcgaaagt gtgatggcag cggcgaaaat cgcccatcaa
    26041 aataagacta tcgttgcgct taacccggct ccggctcgcg aacttcctga cgaactgctg
    26101 gcgctggtgg acattattac gccaaacgaa acggaagcag aaaagctcac cggtattcgt
    26161 gttgaaaatg atgaagatgc agcgaaggcg gcgcaggtac tgcatgaaaa aggtatccgt
    26221 actgtactga ttactttagg aagtcgtggt gtatgggcta gcgtgaatgg tgaaggtcag
    26281 cgcgttcctg gattccgggt gcaggctgtc gataccattg ctgccggaga tacctttaac
    26341 ggcgcgttaa tcacggcgtt gctggaagaa aaaccattgc cagaggcgat tcggtttgcc
    26401 catgctgccg ctgcgattgc cgtaacacgt aaaggcgcac aaccttccgt accgtggcgt
    26461 gaagagatcg acgcattttt agacaggcag aggtgacgct tggctacaat gaaagatgtt
    26521 gcccgcctgg cgggcgtttc tacctcaaca gtttctcacg ttatcaataa agatcgcttc
    26581 gtcagtgaag cgattaccgc caaagttgaa gcggcgatta aagaactcaa ttacgcgcca
    26641 tcagccctgg cgcgtagcct caaactcaat caaacacata ccattggcat gttgatcact
    26701 gccagtacca atcctttcta ttcagaactg gtgcgtggcg ttgaacgcag ctgcttcgaa
    26761 cgcggttata gtctcgtcct ttgcaatacc gaaggagatg aacagcggat gaatcgcaat
    26821 ctggaaacgc tgatgcaaaa acgcgttgat ggcttgctgt tactgtgcac cgaaacgcat
    26881 caaccttcgc gtgaaatcat gcaacgttat ccgacagtgc ctactgtgat gatggactgg
    26941 gctccgttcg atggcgacag cgatcttatt caggataact cgttgctggg cggagactta
    27001 gcaacgcaat atctgatcga taaaggtcat acccgtatcg cctgtattac cggtccgctg
    27061 gataaaactc cggcgcgcct gcggttggaa ggttatcggg cggcgatgaa acgtgcgggt
    27121 ctcaacattc ctgatggcta tgaagtcact ggtgattttg aatttaacgg cgggtttgac
    27181 gccatgcgcc aactgttatc acatccgctg cgtcctcagg ccgtctttac cggaaatgac
    27241 gctatggctg ttggcgttta ccaggcgtta tatcaggcag agttacaggt tccgcaggat
    27301 atcgcggtga ttggctatga cgatatcgaa ctggcaagct ttatgacgcc accattaacc
    27361 actatccacc aaccgaaaga tgaactgggg gagctggcga ttgatgtact catccatcgg
    27421 ataacccagc cgacccttca gcaacaacga ttacaactta ctccgattct gatggaacgc
    27481 ggttcggctt agatttacgc tgtcttttga tcaaattatt accatcggtt gttttcagaa
    27541 gcatgaacat tgctgctgaa gcaacagtaa taatgcccat cgtgataaac gtatagtgga
    27601 attgttcgac agtcgttgtg ccttccatcc cttcataaac gcgaaggacg gccgcactta
    27661 cagcaacgcc taaactaatc gacagttgct gcgtgaccgc cagaacactg ttaccgctgc
    27721 tggcgttgtc atcggtcaga tcggcaagtg tgatggtatt catcgcggta aattgcgtcg
    27781 acatagccat ccctaatata aacaacggca agatcagcat ccatatcgcc attaccggtg
    27841 attgcaaaga gaactgagcg atcattagcc caataatcac cgtgatcccc actaacgtat
    27901 ggcgatagcc cagacgacgt aagacttggg taaccatcga ttttgcaata atggaaccta
    27961 acgctgtcgg tgccatcata cagccggcaa taaacgcctg ataaccaaat cctacctgta
    28021 acatcaatgg cataaggaac ggtacacaac cggtccccag acgggttgca atattgccta
    28081 cgataccgat cgagaaagtg cgggttttaa ataaatctaa tgaaattaat gggtttggcg
    28141 tgtgtcgcgc atggagaata tagagaagca gtaacccgat gctggtgaca attaccgtca
    28201 aggcaatcca gctggcgaca atcttttccc cgaatagctc tattccgctt gagaagagaa
    28261 caaggctgag gccaaacagc aaaaagccag tgatatcgaa tctgcgtcgt gcggtggtga
    28321 aattgggcat atgtttgcgc gcgtaaagaa ggcccgcaat acctatgggg atattgatta
    28381 aaaatatcca gtgccaggtt gcccaggtga ccagcacgcc gccaagaacg gggcctaaaa
    28441 ttggccccac cagacccggc atggcgacaa aattcaatac tggaagaagt tcattacgag
    28501 gataagcgcg cagtaaggcc agccgagcaa caggcatcat cattgcgccg cctatcccct
    28561 gaataacccg gaagacaacc agctgtggta gcgaattaga aagtgcgcag gccagagaac
    28621 ccaatgtgaa cagactcacg gcaagggtaa aaatgcgacg cgtaccgaag cgatcggcta
    28681 gccatccgct taccggaata agcatcgcca ccgtcagcgt ataactgatg atggctgatt
    28741 gcatcgcgag aggagaacga ttaaggctat gagcgattgc gggtaaggcg gtattcagaa
    28801 tagtggcatc aagtgcctgc atgaagaagg ccatcgccgc gatccacggc aaacccgcca
    28861 tactgcgctt ctttttatcg ctcattcaat gtcctattat cgggttgtca cttatcaggt
    28921 gagcgtagca gcgcctgaca agctttaaat gccgcgtcgc catcgctttg gacaatcgca
    28981 tcgacaatcg cctggtgcag atccagcttt atcactgtgt cgctggtaat tgacgtgaag
    29041 taagtgtgat aaaccgaatg gaatagcgag gcgaatgagg tcaaaaacgg attggcgctc
    29101 atttcataga tatgctcatg ccaggccata tcgacttcga tccagcgttc acggcgaaag
    29161 ttctctttta atgccgccat ttcggccatt aacgtattga gatgcgcctt ctgttccgcg
    29221 gtgccaaccg ttgcagccag taggcaagct tgcggctcca gacagatacg cataaccaga
    29281 aagtgatcga tgacctgatg aaagttctct tctgtcatcc accaggtaag caattcctga
    29341 tcaagaaaat tccagtttga ttgtggcatg acccgggtac caattcgcgg tcgcggtaaa
    29401 accatccctt ttgccgttaa cgttttgacc gcttcgcgta ccgctgtacg actcactcca
    29461 aattgctcgc ccagctcaat ctcaccaggc aaaatggtgc cgggttcata ttcacctttt
    29521 aagatgcgtt gcgccagctt ctcagccaga acatacgaaa ggtttttctg tgcagctaac
    29581 tgttgtgcgc ttaaaggcat tactaatctt cctttttctt tttattcctc cttagtatgc
    29641 caccaggaag tgtgattacg gttgcaaaaa cggcaaattg cttgttttat ggcacattaa
    29701 cggggctttt gct
//
LOCUS       DAZGOB010000051        29133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGOB010000051 DAZGOB010000000
VERSION     DAZGOB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.051"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHLIF_002437"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHLIF_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO9606439.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHLIF_002438"
     CDS             complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHLIF_002438"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO9606440.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHLIF_002439"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHLIF_002439"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO9606441.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGELVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="ACHLIF_002440"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="ACHLIF_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO9606442.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="ACHLIF_002441"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="ACHLIF_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO9606443.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="ACHLIF_002442"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="ACHLIF_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO9606444.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="ACHLIF_002443"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="ACHLIF_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO9606445.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHLIF_002444"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHLIF_002444"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO9606446.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHLIF_002445"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHLIF_002445"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO9606447.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHLIF_002446"
     CDS             9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHLIF_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO9606448.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10487..11005
                     /gene="yfiR"
                     /locus_tag="ACHLIF_002447"
     CDS             10487..11005
                     /gene="yfiR"
                     /locus_tag="ACHLIF_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO9606449.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10995..12221
                     /gene="dgcN"
                     /locus_tag="ACHLIF_002448"
     CDS             10995..12221
                     /gene="dgcN"
                     /locus_tag="ACHLIF_002448"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO9606450.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAKKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12237..12719
                     /gene="yfiB"
                     /locus_tag="ACHLIF_002449"
     CDS             12237..12719
                     /gene="yfiB"
                     /locus_tag="ACHLIF_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO9606451.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLTSTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="ACHLIF_002450"
     CDS             complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="ACHLIF_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO9606452.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="ACHLIF_002451"
     CDS             complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="ACHLIF_002451"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO9606453.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="ACHLIF_002452"
     CDS             complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="ACHLIF_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO9606454.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="ACHLIF_002453"
     CDS             complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="ACHLIF_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO9606455.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="ACHLIF_002454"
     CDS             complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="ACHLIF_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO9606456.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTIKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16575..17366
                     /gene="ypjD"
                     /locus_tag="ACHLIF_002455"
     CDS             16575..17366
                     /gene="ypjD"
                     /locus_tag="ACHLIF_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO9606457.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17387..18673
                     /gene="yfjD"
                     /locus_tag="ACHLIF_002456"
     CDS             17387..18673
                     /gene="yfjD"
                     /locus_tag="ACHLIF_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO9606458.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="ACHLIF_002457"
     CDS             complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="ACHLIF_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO9606459.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19444..20322
                     /gene="nadK"
                     /locus_tag="ACHLIF_002458"
     CDS             19444..20322
                     /gene="nadK"
                     /locus_tag="ACHLIF_002458"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO9606460.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20408..22069
                     /gene="recN"
                     /locus_tag="ACHLIF_002459"
     CDS             20408..22069
                     /gene="recN"
                     /locus_tag="ACHLIF_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO9606461.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22218..22559
                     /gene="bamE"
                     /locus_tag="ACHLIF_002460"
     CDS             22218..22559
                     /gene="bamE"
                     /locus_tag="ACHLIF_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO9606462.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22621..22911)
                     /gene="yfjF"
                     /locus_tag="ACHLIF_002461"
     CDS             complement(22621..22911)
                     /gene="yfjF"
                     /locus_tag="ACHLIF_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO9606463.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="ACHLIF_002462"
     CDS             complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="ACHLIF_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO9606464.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23509..23991
                     /gene="smpB"
                     /locus_tag="ACHLIF_002463"
     CDS             23509..23991
                     /gene="smpB"
                     /locus_tag="ACHLIF_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO9606465.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24206..24568
                     /gene="ssrA"
                     /locus_tag="ACHLIF_002464"
     tmRNA           24206..24568
                     /gene="ssrA"
                     /locus_tag="ACHLIF_002464"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24839..25087
                     /locus_tag="ACHLIF_002465"
                     /pseudo
     CDS             24839..25087
                     /locus_tag="ACHLIF_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            25745..27106
                     /gene="espK"
                     /locus_tag="ACHLIF_002466"
     CDS             25745..27106
                     /gene="espK"
                     /locus_tag="ACHLIF_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO9606466.1"
                     /translation="MLPTSQLRPSGTFYSNSAETSAGIKSEITPTQIEEARAIGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIISEGIECLTVCHCHISGVPESVRHLEIRGYATDGIKNVPNGLLSL
                     SINRYTPENQARIDSLISPSLQTLSLTGCSNIILPEKLPESLRSVTIHVEQKTTWNIV
                     AEGVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAALHFRPGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIVIQNTLTNAVWDARTPRKYKQDSLIKRALNEHERGTKFKQCLKNH
                     NNYNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRTVMFCADGLVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNSQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            complement(27483..>29133)
                     /gene="espN"
                     /locus_tag="ACHLIF_002467"
     CDS             complement(27483..>29133)
                     /gene="espN"
                     /locus_tag="ACHLIF_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HFO9606467.1"
                     /translation="NPKPRNARISKELLLLAVDRQIEWAKKHFITKDVLENVVSKCDL
                     SSIFNVNKVLQNTILEFVHEINNISSARWMSKSEKNNKQKEAIEKFKKEVSHMNGGQQ
                     FIWGFDKVIQEGLSGLIELSIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAVP
                     GYNALSGVSSILHSAIVKESSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQIKYEALK
                     HIEKNINDNIIYHPSNNANFYSVIESIDGNDFIYNEKQSKILEMKQDRGGNRYSAVDL
                     NSSKYGYYEKVGGGFYRYIESFNPISSETPNKIVYKGESVDLTKEPNSELYSGRYSIN
                     NKQVNVYFFRDADGTFYKSEGLHGGGVIRYIDKPYSQLREGDIGYDEDLLDIYDDSPV
                     LEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYKT
                     AGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYTSDLSVADRFSREHYLIVVKVKAKYI
                     TRGSVTESGWVIDKTAPVEPLAIIDRTFGMKENISMVNASK"
BASE COUNT         7720 a         7476 c         6562 g         7375 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa agcatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacgcctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccgg
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagcga gtcatacggg ggcaagctca cgccgccgat acgagaagag ctacaggccg
     3601 caacaccttt tggtagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagca ttgatggcaa ccagctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ctattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg atagtgtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgtc agctgcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcagaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggttcta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaacc
     9901 cttacttctg tatagataaa tttacactcc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
    10321 caatacgatt aaattaatta aaaccccaca aataaattaa acataagatt ttactcattg
    10381 tgttttattt ctcacattga tgacgatcgc atgaaaaaat gatattttaa tgagcattct
    10441 gaaaaaatat cgatgcattt cgagcgaaga tggtggggat ccctgaatgc gtttttctca
    10501 ccgactgttc ttacttctga ttctcctgct gacgggagca ccaatactcg ctcaggagcc
    10561 ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg
    10621 gcctgcgcta tcagggccac caaagttatg tatcttttca tcttcacgtt ttagtaccgc
    10681 cctacaagag aatgccgcga catctttgcc ttatctgcct gtcatcattc acactcaaca
    10741 agaggcgatg atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca
    10801 gatggaatta accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac
    10861 cgagtgcatt attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc
    10921 cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct
    10981 cgcacggaag aaaaatgatg gataacgata attctcttaa taagcgcccc acgtttaaaa
    11041 gagcattacg caacatcagt atgaccagca tatttatcac tatgatgctg atctggttgc
    11101 tgctttccgt gacctcggtg ctgaccctga aacagtacgc gcaaaaaaac ctggcactga
    11161 cagcagcaac aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag
    11221 caactgaaac actggcagcg ctgggccagc aagggcaatt ttcaactgca gaagtacgtg
    11281 ataagcagca aaatattctg gcgtcctggc attacacccg taaggatcca ggcgatactt
    11341 tcagcaattt cataagccac tggctcttcc ccgcccccat cattcagccg attcgtcaca
    11401 atggtgaaac cattggcgaa gtacgcttaa ccgctcgcga cagttcaatc agccatttta
    11461 tctggttttc gctcgccgta ctgaccggtt gtattctgct ggcatcaggc atcgcaatta
    11521 ccctcacccg ccatttgcac aatggcctgg tagaagcgct gaaaaatatc accgatgtcg
    11581 tacatgatgt gcgttccaac cgcaattttt cccgacgagt ttcggaagaa cgtatcgctg
    11641 agtttcaccg cttcgctctc gacttcaaca gtctgctgga tgaaatggaa gagtggcagc
    11701 ttcgtttaca ggccaaaaat gcgcagcttc tgcgtaccgc gctacatgac ccattaaccg
    11761 ggctggctaa ccgcgcagcg tttcgtagcg gcatcaatac gttgatgaac aattccgatg
    11821 cccgaaaaac gtcggcgtta ctatttcttg atggcgataa tttcaaatat atcaatgata
    11881 cctggggtca tgcgacgggc gatagagtct tgattgaaat cgcaaaacgg ttagctgaat
    11941 ttggcgggct gcgacataaa gcataccgcc tgggcggcga tgaattcgct atggtgctct
    12001 atgatgtaca gtcggaatct gaagtgcagc agatatgctc agcactgaca caaatcttta
    12061 atctcccgtt tgatcttcat aatggccatc agaccaccat gacattaagc attggttacg
    12121 cgatgaccat tgagcacgcc tctgcgaaaa aattacaaga gcttgccgat cacaatatgt
    12181 atcaggccaa acaccagcgt gccgaaaagc tggtgagata acaaggatat atcgatatga
    12241 taaagcacct ggtagcaccc ctgattttca cctcactaat actgactggc tgccagtccc
    12301 ctcagggaaa gtttactcct gagcaagtcg ccgctatgca atcttatgga tttactgaat
    12361 ccgccggcga ctggtcgctg ggcttatcag atgccattct gttcgcaaaa aatgactaca
    12421 aattgctccc ggaaagccag caacagatcc aaaccatggc agccaaattg acctcgacag
    12481 ggctaacaca tgcccgtatg gatggacaca ccgataacta tggtgaagac agttacaacg
    12541 aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc atgggctatg ggtggacaaa
    12601 ttccgcgcag caatctcacc acacagggtt taggaaaaaa atatcccata gccagtaaca
    12661 agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt ggtgattact accccttaaa
    12721 acaccagcaa acagataaaa aaagggccag ccaattggcc agcccttctt aacaggatgt
    12781 cgcttaagcg aaatcttagt taagacgctc tttgatacga gcagccttac cagtacgctc
    12841 acgcaggtag tacagtttag ctttacgaac agcaccacga cgtttgacag aaatgctgtc
    12901 aactaccgga gagtgagtct ggaagacacg ctcaacgcct tcgccgttgg aaattttacg
    12961 aacagtgaat gcagagtgca gaccgcggtt acgaatagcg ataaccacgc cctcgaatgc
    13021 ctgcagacgt tttttggaac cttcaacaac ccatactttc acttccacgg tatcacccgg
    13081 acggaaggaa ggtacgtcct gcttcatctg ctcttgttca agttgcttaa taatgttgct
    13141 cataatttaa tctcttatcc tgggtaaact gatatctcgg gggcttacgc catcccatca
    13201 tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg ccagcaacct tgcttgctct
    13261 tcagtcagag ccaggttttc cagaagttca ggtcttctaa gccaggtacg gcccagcgac
    13321 tgtttcaaac gccagcgacg tatctcggca tggttgcccg acagtaacac tggcggaact
    13381 tccatccctt ctaacacctc aggccgcgta tagtgcgggc aatccagcaa tccttcagca
    13441 aaggaatctt ccgttgccga ggcttcatgt cccagtactc ccggaataaa ccgggaaacg
    13501 gagtcaatca gcgtcattgc tggtaactca ccaccactga gaacgtaatc gccgattgac
    13561 cattcttcgt caatttcggt ttggatcacg cgctcatcta taccttcgta gcgaccgcac
    13621 accagaatca atttttgatt cgttgccagt tcgctgacgc ccgcttgatc aagcttgcgt
    13681 ccctgtggtg acagataaat cacctttgcg ccttcacccg ccgcggcttt tgctgcatga
    13741 atggcgtccc gcaagggttg caccatcatt aacatccccg gtccgccgcc gtaaggacga
    13801 tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag gactccaact ctggatgctc
    13861 agcaggccat ttttaactgc ccggccagtt accccgtaat cggtaattgc gcggaacatt
    13921 tcaggaaaca ggctaattat gccaatccac atagcgccgt cttttaccgt ttatccggtg
    13981 gtttaaaaac caggatccca atctacttcg attgaacgag tagtgagatc gactttcttg
    14041 ataacctgcc catcgaggaa cggtacgaga cgttccttga taccaaacgc atctttcagg
    14101 tttgccttaa tgacgagaac gtcattagat ccggtttcca tcatatcgac gactttaccg
    14161 agatcgtagc cttcagtggt tactacctgg cagcccatca ggtctttcca gtagtagtcc
    14221 ccctcttcaa gctgaggcag ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg
    14281 ttcgccgcat cacgatcgtc aacgcctttc agcttgatga tcatgtcctg attgtggtgc
    14341 ttccagcttt ccagctggac ttgctgccac tgacccgcct tctggataaa ccagggctga
    14401 tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca ctctgagcca cccacgaata
    14461 ccgtaagacg aacccatttt tcccaaaacg atgggatcaa caggtgcttg cgcggtgagt
    14521 tgtttgctca tcatgaccac cgtgacagat taagctgctt tgtttacttc tttgatcagc
    14581 gcagcaacgc gatcagaaat agttgcgccc tggccaaccc agtgagcgat gcgatccaga
    14641 tccaggcgag tgccttcttc tttttcgcta gcgattgggt tgaagaaacc aacgcgctcg
    14701 atgaagcgac cgttgcgtgc attacggctg tcagcgacaa caacctggta gaacggacgc
    14761 tttttagcgc cgtgacgtgc taaacgaata gttaccataa catcctcttg tgtgaataaa
    14821 acaaccggac cccatcgagg aacggagtcc ggtgtcatat taaaagcccg aaaattttac
    14881 tcatttttgc gggaattgca atcaacagtt gctaactctg ctgtaaaagg ccgtcggcgg
    14941 tgcagccagt ttggtgccgg agtgcgcgca gtcaccggag cgtacacgca gtacgtgagg
    15001 atgacgagca catcccggtg ccaaaatggc aaacaagcca ggccgattag cgaccaggga
    15061 agcctggggg catcataccc ttcatgcttc tcatcatctt cgccattccg cctttcttca
    15121 ttttcttcat catgcgctgc atgtcgtcga actgcttcag aaggcggttc acgtcctgca
    15181 cctgcatacc gcaaccggca gcgatacgac gcttacgcga acctttgatg atttctggct
    15241 tagcgcgctc ttttatcgtc atcgagttga tgatggcttc catacgcacc agcactttat
    15301 cgtccatctg cgacttaaca ttatccggga tctgccccat gcccggcagc ttgcccatca
    15361 ggctagccat gccgcccata tttttcatct ggcgcagttg ctcaagaaaa tcgttgagat
    15421 cgaagccgtc gccttttttc agcttgctgg ctaatttctc tgcctgcgcg cggtcaactt
    15481 tgctttcgat atcttcgatc agcgacagta cgtcgcccat gccgagaata cgcgacgcga
    15541 tgcggtccgg atggaacggc tccagcgcct cagttttctc gccaacaccg agaaacttga
    15601 tcggtttgcc agtgatgtga cgaatagaga gcgccgcacc gccgcgggca tcgccgtcca
    15661 ctttggtcaa cactacgccg gtaagcggta acgcttcatt gaatgctttt gccgtattgg
    15721 ccgcatcctg accggtcatg gcgtcaacca caaacagggt ttcaaccggg ttaatcgacg
    15781 catggacttg tttgatctcg tccatcatcg cttcgtcaac gtgcagacga ccagcggtat
    15841 ccaccagcag cacgtcgtag aatttcagtt tggcttcttt cagcgccgcg ttaacgatat
    15901 ctaccggctt ctgaccaaca tcagaaggga agaaatcaac gcccacctgc tctgccagcg
    15961 tctcaagctg tttgattgcc gccgggcgat aaacgtcggc agaaaccacc agcactttct
    16021 tcttgtgctt ctcgcgcagg aacttaccga gtttaccaac gctggttgtt ttaccggcac
    16081 cttgcaggcc cgccatcagt acgaccgcag gcggttgcgc agccaggttc agggtctggt
    16141 tctcttcgcc catcgccgca accagttcgt tacggactat tttgacgaac tcctgccccg
    16201 gcgtcaggct cttattaact tcatgaccaa ccgctttctc ttttacgcga ttgataaact
    16261 cacgcactac cggcagagcg acgtccgcct ccagcagcgc catgcgcact tcgcgcagcg
    16321 tatcttttac gttgtcttca gtgaggcgtc cacggccact gatattgcgc agcgtgcgcg
    16381 acaaacgatc ggttaaatta tcaaacattg tctctcgcct ggggtggaaa cggttggccg
    16441 caatcgcgac acatcatcag tattttgccg cagtataaca tgaaggcgtc tttgttgtta
    16501 tgcaacggtt ggagcagcgt tcacctgacg ctatactgct tctctttctt attgctcaaa
    16561 ctgtcgacat tactatgccc gtttttgctc tgctcgcgct tgtcgcctac tccgtcagtc
    16621 ttgcgctgat tgttcccggt ctgctgcaaa aaaacggcgg ctggcggcgc atggctatta
    16681 tttctgcggt cattgcgctg gtctgccacg caatcgctct ggaagcccgc atcctgcccg
    16741 acggtgatag cggacaaaac ctcagcctgc tgaacgttgg ttcattggtc agtttgatga
    16801 tctgtacggt aatgaccatt gtggcttctc gcaatcgtgg ctggttgctg ctacccattg
    16861 tctatgcctt tgcgcttatc aacctggcgc tggcgacctt catgcccaat gaatacatca
    16921 cccatctgga agcaacgcct gggatgctgg tgcacattgg cttatcgctc ttttcctatg
    16981 ccacgctgat aattgccgcc ctgtacgcgc tacaactggc gtggattgat taccaactga
    17041 agaacaagaa gctggcgttt aaccaagaaa tgccgccatt gatgagtatc gagcgtaaaa
    17101 tgttccacat cacgcagatt ggcgtggtgc tactaacgct cacgctttgc actggcctgt
    17161 tctacatgca caacttattt agcatggaaa atatcgacaa ggccgtgctc tctatcgtgg
    17221 cgtggtttgt ctatattgtg ctgctgtggg gacattatca tgaaggatgg cgtggacgcc
    17281 gcgtcgtctg gtttaacgtt gcgggtgcag tcatcctgac actggcctac ttcggcagcc
    17341 gaattgtcca gcagttaatc agctaaaccc agaaaaggag tttcccctgg aacacatttc
    17401 tactactacg ttgatcatta ttctgatcat catggtggtc atttcagcct atttttccgg
    17461 gtccgaaacc ggaatgatga ccctcaaccg ctatcgtctg cgacatatgg cgaaacaggg
    17521 taatcgctcg gccaaacgcg tcgaaaaatt gctgcgtaag ccagaccgcc tgataagcct
    17581 ggtgttaatc ggcaataacc tggtcaatat tcttgcctcc gcgctcggca ctattgttgg
    17641 gatgcgtttg tacggcgatg cgggcgtggc aattgcgact ggtgtgctga cttttgtcgt
    17701 actggttttt gctgaggtat tgccgaaaac cattgccgcg ctgtacccgg aaaaagtcgc
    17761 ttatccgagt agttttctgc tggctccgct gcaaattttg atgatgccgc tggtctggtt
    17821 gctgaatgct atcacccgta tgctgatgcg catgatgggt atcaaaaccg atatcgtggt
    17881 tagcggctct ttgagcaaag aagagttgcg cactatcgtg cacgaatcac gctcacaaat
    17941 ttcccgtcgc aatcaggata tgctactgtc tgtgctcgat ctggaaaaga tgaccgttga
    18001 tgacatcatg gtgccgcgca gtgaaattat cggtattgat atcaacgatg actggaaatc
    18061 gattctgcgc caactctccc actcacctca cgggcgcatc gtgctctacc gtgattcgct
    18121 ggacgacgcc atcagtatgc tgcgagtacg tgaagcctgg cggttgatgt cggagaaaaa
    18181 agagttcacc aaagaaacca tgctgcgcgc cgcggacgag atctattttg tgccggaagg
    18241 tacgccgctc agcacgcagt tggtaaagtt tcagcgcaac aaaaagaaag tcggcctggt
    18301 cgtcaacgag tatggagaca ttcaggggct ggtgacggtt gaagatattc tggaagagat
    18361 tgtcggcgat ttcactacgt cgatgtcgcc aacacttgcc gaagaggtca cgccgcaaaa
    18421 cgacggttcg gtgattatcg atggcaccgc caacgtgcgg gaaatcaaca aagcctttaa
    18481 ctggcatcta ccggaagatg atgcccgcac ggttaatggc gtcattcttg aggcactgga
    18541 agagatccct gtcgcaggca cccgcgtgcg tattggcgag tacgatatag atattctcga
    18601 cgtacaggac aatatgatta agcaggtaaa agtttttcct gtgaaaccgc tgcgcgagag
    18661 tgtggcggag taacgaaaac ggccctgcat tcgcatgcag ggccgtgaat tattacgaaa
    18721 acagaaatta cgcttttgct ttcgctacag ttaccatcgc cgcacgaatc gtacgaccat
    18781 tcagcgtata acccttctgc ataatgccca gtacgttacc tggcgcaacg tcatcagatt
    18841 ccaccattgc gatagcctga tgcacattcg ggtccagcgg cacgttagtt tcggcgatca
    18901 cttcaacacc aaacttacgc acaacatcca gcatcgactt cagcgtcagc tcaatgcctt
    18961 caaccatcgc agacatatcc gggttagctt tatcagccac ttccagcgca cgatccaggc
    19021 tatcaatcac cggcagcaat tcgttgatga atttctccag cgcgaatttg tgggcttttt
    19081 caatatccag ttcagtacgt cgacgcaggt tttccatttc ggcttttaca cgcaaaatgc
    19141 cgtcacgttc acgggtctgg gcttcagcca gctgagcttc gagattcgca attttttcat
    19201 cgcgcggatc cacctgctca gcagaagctt ctggctcaac tgcctcaatc tcttcgtgct
    19261 gatccatgat aatttcttcc ggggcttgcc cctcaggcgt tttctgttct ttactactca
    19321 tgaatttctc cgcgtttttt tcgcattcat ctcgctaact tcgcttatta tggggatcag
    19381 tttcagggtt tcaagggaag cactcacatt gtcatcaatc ttcgcaacaa ggacctcgga
    19441 aaaatgaata atcatttcaa gtgtattggc attgtgggac acccacggca ccccactgca
    19501 ctgacaacac atgaaatgct ctaccgctgg ctgtgcacaa aaggttacga ggtcatcgtt
    19561 gagcaacaaa tcgctcacga actgcaactg aagaatgtga aaactggcac gctcgcggag
    19621 attgggcaac tagctgatct cgcggtagtc gttggtggcg acggtaatat gctgggcgcg
    19681 gcacgcacac tcgcccgtta cgatattaaa gttattggaa tcaaccgtgg caacctgggt
    19741 ttcctgactg accttgaccc cgataacgcc cagcaacagt tagccgatgt gctggaaggc
    19801 cactacatca gcgagaaacg ttttttgctg gaagcgcaag tctgtcagca agattgccag
    19861 aaacgcatca gcaccgcgat aaatgaagtg gtgcttcatc caggcaaagt ggcgcatatg
    19921 attgagttcg aagtgtatat cgacgagatc tttgcgtttt ctcagcgatc tgatggacta
    19981 attatttcga cgccaacagg ctccaccgcc tattccctct ctgcaggcgg tcctattctg
    20041 accccctctc tggatgcgat taccctggtg cccatgttcc cgcatacgtt gtcagcacga
    20101 ccactggtca taaacagcag cagcacgatc cgtctgcgtt tttcgcatcg ccgtaacgac
    20161 ctggaaatca gttgcgacag ccagatagca ctgccgattc aggaaggtga agatgtcctg
    20221 attcgtcgct gtgattacca tctgaatctg attcatccga aagattacag ttatttcaac
    20281 acattaagca ccaagctcgg ctggtcaaaa aaattattct aattttacgc cagcctcttt
    20341 actgtatata aaaccagttt atactgtaca caataacagt aatggttttt catacaggaa
    20401 aacgactatg ttggcacaac tgaccatcag caactttgct atcgttcgtg agcttgagat
    20461 tgattttcat agcggcatga ccgtaataac tggcgagacc ggcgcgggta aatctattgc
    20521 aatagatgcc ctcggtcttt gtctcggtgg tcgcgctgaa gccgacatgg tgcgtaccgg
    20581 cgctgctcgc gctgacctgt gcgcccgttt ttctctgaaa gatacgccag cggctctgcg
    20641 ctggctggaa gaaaatcagc ttgaagacgg gcatgaatgt ttgcttcgtc gagtgatcag
    20701 cagcgatggt cgctcccgtg gtttcatcaa cggtacagct gttcctctgt cacaactgcg
    20761 cgaactgggt cagttgctga ttcagatcca tggtcagcac gctcatcaat tactcaccaa
    20821 acctgagcac caaaaattcc tgcttgatgg ctatgccaat gaaacctctc tactgcagga
    20881 aatgaccgca cgttatcagt tgtggcatca aagctgccgt gacctcgcgc atcatcaaca
    20941 gttaagtcag gaacgcgccg cccgtgcgga actgctgcaa taccaattaa aagaacttaa
    21001 cgaatttaat ccgcagcccg gagagtttga acaaatcgac gaagagtaca aacgtctggc
    21061 gaacagcggt caattgctga ccaccagcca gaatgcattg gcattaatgg ccgacggtga
    21121 agacgcaaac ctgcaaagtc agctttacac ggctaaacaa ctggtgagcg aattgattgg
    21181 catggacagc aaactgtccg gcgtacttga tatgctggaa gaagctacca tccagattgc
    21241 tgaagccagc gatgaactgc gccactactg cgatcgtctg gatctcgatc ccaaccgact
    21301 atttgaactt gaacagcgca tctcaaaaca gatttcgctg gcacgtaaac atcacgtcag
    21361 ccctgaggca ttgccacagt attaccagtc gctactggaa gaacagcagc aactggacga
    21421 tcaggccgac tcacaagaaa cgcttgcgct ggcggtaacg aaacatcatc agcaggcact
    21481 ggaaatcgcg cgcgcattac accaacaacg ccagcaatat gcagaagaac ttgcacagct
    21541 gatcaccgac agtatgcatg cgctctcaat gccgcatggg cagtttacga tcgatgttaa
    21601 atttgacgag catcacctgg gcgctgacgg tgccgatcgt attgagtttc gggtaaccac
    21661 caacccaggt cagccaatgc agcctattgc caaagtcgca tccggtggtg aattgtcccg
    21721 catcgcactg gcaatccagg tcatcacggc gcgtaaaatg gaaaccccgg cactgatttt
    21781 tgatgaagtg gatgtaggga ttagcggtcc aacagcggca gttgtcggca aactgctgcg
    21841 tcaactcggc gaatcaactc aggtgatgtg tgttacccac ctgccacaag tcgcgggatg
    21901 tggtcatcaa cactattttg tcagcaaaga aaccgatggt gcgatgacag aaacgcatat
    21961 gcaatccctg aataaaaaag cgcggttaca agagctggcg cgcctgcttg gtggcagtga
    22021 agtcacacgt aatacactgg cgaatgcgaa agaactgctt gcagcgtaaa cttttttcct
    22081 gcttcacggt cagagtaaac agcaaaacgc cgtaagaccg gaaagcaaaa ggttttaaag
    22141 tgatgaaagg tctattatca tcggcatatt acagatgagc cacgtactgc tcgggcccga
    22201 aaaggaatca aatcactatg cgctgtaaaa cgctgactgc tgcagcagca gtactattga
    22261 tgttgaccgc aggctgttcc actctggagc gagtggttta ccgtcctgac atcaaccagg
    22321 ggaactatct gaccgctaac gacgtatcca aaatacgtgt tggcatgacg caacaacaag
    22381 ttgcgtacgc attgggtaca ccgctgatgt ccgatccatt tggtacgaat acctggttct
    22441 atgtcttccg ccagcaacca ggtcatgaag gtgtaacgca gcaaacgctg acgctgacct
    22501 ttaacagtag cggtgtgttg accaatattg ataacaaacc tgcgctgagt ggtaactaat
    22561 aataaagttg ctctcaaaga cgttaaaaaa ggtgctcaat gagcaccttt tttctgtctg
    22621 ttatttattc gctgattttt ctgctcgttg cctgcgaagc tctttcggat cggcaatgag
    22681 agggcgataa atctccaccc gatcgccatc atgcacacta tcgcttagtt ttgccggacg
    22741 gctgtaaatg ccgactttgt ttttagttaa atcgatatcg gtacgcaatt ccagcaagcc
    22801 actggcgcga atagcttctt caaccgtcgc gccctcctgc agcgtcacgc gctgcaggta
    22861 ctgcttctca ggtagcgcat aagccacctc aacggcaatt ttacctggca ctgtaaacct
    22921 ctttcgcacg aaccgtaaaa gcctggacca tattagacgc cagctcttta aacacgcgac
    22981 caaaggcgag ttcaatcaac ttattggtaa actcaaagtc gagatgaaac tcgatacgac
    23041 acgcctcctg gctcagcggc gtaaacttcc atccaccaat caatttcttg aacggcccat
    23101 ccaccagatt cataagaata ctttggttac tggtcaactg gttgcgggta gtaaacgttt
    23161 tgctgatccc agccttagag acatctaccg ccgcagtcat ctgcccagga gtggactcca
    23221 gaatccgact tccggtacaa cccggcaaaa actgaggata agactgaacg tcattcacta
    23281 actgatacat ttgctccgcg ctgtagggta ccagtgcggt ccggctaatc tgaggcataa
    23341 caatttccat caacaaaaat ccaacaaata atatcattta tcctgctgta aaaaaaacgc
    23401 tatcccggcg ctgggtaaca tcgggttcat gctaagatag agccttgtcc cccgcaggat
    23461 tgatatgggg tgttttcgat ttcagattac cgatgattca cgacgcttat gacgaagaaa
    23521 aaagcacata aacctggttc agcgaccatc gcgcttaaca agcgcgcccg tcacgaatac
    23581 tttatcgaag aagagttcga agcgggactt gccctgcaag gctgggaggt taaatccctg
    23641 cgcgcaggaa aagccaatat cagcgacagc tacgtccttc tgcgtgacgg agaggcattt
    23701 ctgtttggcg ctaacatcac gccaatggcc gtggcctcca cgcatgtggt gtgcgatcct
    23761 acccgtaccc gcaagttact tctcaaccag cgcgaactgg actcattgta cggtcgcgtc
    23821 aatcgagaag gctataccgt agtggcgctc tccctgtact ggaaaaatgc ctggtgcaaa
    23881 gtgaaaatcg gcgtcgccaa aggtaaaaaa cagcacgata aacgttcaga tatcaaagaa
    23941 cgcgaatggc aggtggataa agcacgtatc atgaaaaacg cccaccgtta aacctgcact
    24001 ccaattattg accagttcct caccgcgcct ccctctccgg cggcgcgaat gaacatctta
    24061 ttggctatca catccgacac aaatgttgcc atcccattgc ttaatcgaat aaaaatcagg
    24121 ctacatgggt gctaaatctt taacgataac gccattgagg ctggtcatgg cgctcataaa
    24181 tctggtatac ttacctttac acattggggc tgattctgga ttcgacggga tttgcgaaac
    24241 ccaaggtgca tgccgagggg cggttggcct cgtaaaaagc cgcaaaaaat agtcgcaaac
    24301 gacgaaaact acgctttagc agcttaataa cctgcttaga gccctctctc cctagcctcc
    24361 gctcttagga cggggatcaa gagaggtcaa acccaaaaga gatcgcgtgg aagccctgcc
    24421 tggggttgaa gcgttaaaac ttaatcaggc tagtttgtta gtggcgtgtc cgtccgcagc
    24481 tggcaagcga atgtaaagac tgactaagca tgtagtaccg aggatgtagg aatttcggac
    24541 gcgggttcaa ctcccgccag ctccaccact tttgatagga ctgcaatcgg acagcggcaa
    24601 taaaaacagc cacttacgga ctctgaccag acagagcgca gaccgagaaa agacaaaaaa
    24661 atgcacgtaa aatgcacgtg cactttaaaa gaaccccaga tctcacggtc tggttttttt
    24721 ctgtttgtaa ctaatggtaa caaaaacaca tcacctcttg gcgttccttc tccttgatac
    24781 tgtttatcca tacagttaaa aataacactg tatacaaaca cagtatagag agatttttat
    24841 gcgtattgaa atttgcatag ccaaagaaaa aatcagcaaa atgccaaaag gatctgttcc
    24901 tgccctacag caagaaatgc tgtgacgtgc cagtaaacgt tatgacgatg tagaagtgat
    24961 catcaaatcc accagcaacg atggcctttc agttactctc accgccgata aagattctgc
    25021 aaaaactttt gttcaggaga cgctgaaaga tacctgggag tctgctgacg agtggtttgt
    25081 tcgctaatta acgagtaaaa tcagtaacgg ctggaaatca ttcaatactc acactatcta
    25141 aaagttcacc agccaacagc ggtaagttct ttcatacgac gtgccgcgga tcctattatt
    25201 ctagtatgaa atccctaacc gatttcaaat tctccagact gctcagattc tcttttatcc
    25261 cacgctggcg ctgataaatc tagtcattgc aatcgacctg cgctatcgct gccgcagttc
    25321 ttccagtttc tgcatcgata actccatctt ctgaataaga aggcaacaga atagataatt
    25381 agtgctttat attcaaaagg ttaatagcaa atttggcatg ctttttactg tctagtaaac
    25441 agcatgcctc tatccataaa atcaaacagt tgcaatctaa ttttggagaa cgttttttct
    25501 tctccctttt agcaaatcgc tataaaaaac aacaaatggt ataaaaatga gcaatcctaa
    25561 tccatgcatg acggactgga gaaagtattc gcaaaagtta aaatcaacag tctgttgatt
    25621 ttgagatata actctctttt ctaatattga caccttggct ttgctggttg ctaaaattag
    25681 cttgttgatt agcagtatgg atagaactta cactgttatt tacttctaaa aatggaattt
    25741 atcaatgctt cctacatcgc aattacgacc gtccgggaca ttctactcca attccgcaga
    25801 aacatcagca ggcatcaaaa gcgaaatcac accaactcag atagaagaag cgcgggccat
    25861 tggtcgttta tatatcaaag attgtgatat tgagtatctg ccacagttac caaacgaaat
    25921 aacatcagtt acaatcgaaa attgcaataa cctgaccacc cttacaggat taccggttaa
    25981 tacacaaaac ctgtccgtca ttaactgtga aaaattacaa atcacagaca taccatcgac
    26041 agtaaaaaat ctacatattg aattaactga ttcaccattt atacacatca tatctgaagg
    26101 catcgaatgc ctgacggttt gccactgcca tatatctgga gtgccagaga gtgtccgtca
    26161 tcttgagatc agaggttacg ccacagacgg cataaaaaat gttccaaacg ggttattatc
    26221 tctcagcatc aatcgctaca ccccagaaaa tcaggccaga attgacagcc tgatatcacc
    26281 gtcactacag acgctatctc tgactggatg tagcaatatt atactgccgg agaaactccc
    26341 ggagagtctg agatctgtaa ccattcatgt ggagcagaaa actacatgga acatcgtagc
    26401 tgaaggggtt cctgatggac tggatcttga tttacaaaat gtactactct ctccgaatgt
    26461 aattaaagaa aaaaacatca cttttcaggg caacgcactg gatgctgcct tgcattttcg
    26521 tccgggagac attgtctatg ggctatcctc tcccagagaa aaacttgtca acagcatcaa
    26581 actggttaac gacttttcca aaaaagatat cgtaattcag aatacattaa caaacgcagt
    26641 atgggacgcc agaacccccc gtaaatataa gcaagactcg cttatcaaaa gagcattaaa
    26701 tgaacatgaa agaggaacaa aatttaaaca gtgcttaaaa aatcacaata attataatgt
    26761 catcatggcc gacctttcca tatacaatcg agacaagtta tgggcaaaaa caagcaaggc
    26821 aggcctagag tttcaaacat taattcgcaa cagaacggtt atgttttgtg cggatgggct
    26881 tgtcaattca ctcaaactca tagctaacaa gtcagagggc tatggtcaga gtattaccgc
    26941 cagcgaatta cgatggattt atcgtaataa agacaacagc caaataatga aaaacataaa
    27001 attttatctg catggcaaag agataccagc agaaagaata ttagatacac cagagtggaa
    27061 agactaccgt ccaaaatact ccggttccac atataaatat tcttaatgat agcaaaaaat
    27121 atattttcga tataatcaat gttatgatta aagagtattt catcagggca ggtaaaaaca
    27181 gagtaaatca gcaaaagaag ctgatcttca gcgatactga cactaactga cggtttaagc
    27241 ggtcgtatga agcagcagct ttccgacgga ctgccatgcg gatcgtttac cttttgggct
    27301 attccgcccg tcatcaagcg gctcacgagt actgagttta tcaggatgat attgctgaca
    27361 atggtaattc gttgaccgat gtgtacttca ctatatatcg gtcaacaccg tactgccgtg
    27421 caacatctca aagatgtagc atcgttttta ccagaggagg caggacatag attgtaaaaa
    27481 aactatttcg atgcatttac cattgagata ttttccttca taccaaaagt tctatcaatt
    27541 atcgcaagtg gttcaacagg tgcggtctta tctataaccc aaccactctc tgtaacactt
    27601 cctcgtgtga tatattttgc ctttacttta acaactatta gataatgctc acgactaaac
    27661 cgatcagcaa cgctaagatc cgatgtgtat tcaggcaata ttcttccaat acgtacctgc
    27721 tgttgtgcca tatattcatc tggtgctttt acatttggat ttgaatcagg aagccctcca
    27781 gcagttttat attcaacgat attttcagct tgaataccgg ttgtgccgcg acacaatatg
    27841 atgtctgaat cggacagttc tatgtgcccc tccttaattt tcgaataaat ttgtttaata
    27901 ctatgttctg gagttggtac tatttcagaa gataaagcag gcaacgtgtc ttcaagcacc
    27961 ggagaatcat cgtatatatc caacaaatcc tcatcatacc caatatctcc ttctcttaac
    28021 tgagaatacg gtttatctat gtatctaata actcccccac catgaagacc ttctgattta
    28081 taaaatgtac catcagcgtc acgaaagaaa taaacattaa cctgtttgtt atttatagaa
    28141 tacctacctg aatataattc cgaatttggc tccttagtta aatctactga ttcccccttg
    28201 tagactattt tatttggtgt ctctgaagat atggggttaa aggattctat atatctataa
    28261 aaaccaccgc caactttctc ataataccca tacttagaag agttaagatc tactgcacta
    28321 tatctattcc ccccacgatc ctgtttcatt tctaatattt tagattgttt ttcgttatat
    28381 ataaagtcat taccatcaat tgactcaatt acagaataga aattagcatt attactcgga
    28441 tgataaataa tattatcatt aatgtttttt tctatatgct tcaaagcctc atattttatc
    28501 tgcagcgaac ggctatgtaa gtcagagcga gtgcctggaa caaagtccat tccaatacgt
    28561 acagccccct gaatataatc acagatattg ctagattctt ttactatagc actatgtaaa
    28621 atgctactta caccagacaa tgcattataa ccaggtacag ctcccatcgc catattccaa
    28681 attgtaccta aaaaatgtaa cacagctctc ccatcaggag aaagagaaga cttatcacga
    28741 tgatttgtac tatcattaat atcgatactt aactcaatca atccacttaa gccttcttga
    28801 ataaccttat caaaccccca aataaactgc tgcccgccat tcatatggga tacttctttt
    28861 ttgaactttt ctattgcctc tttttgttta ttattctttt ctgattttga catccagcga
    28921 gcagatgata tattattaat ttcatggaca aactcaagaa tagtattctg aagcacttta
    28981 ttaacattaa agatagatga taaatcacat tttgacacaa cattttccaa tacatctttt
    29041 gttataaaat gttttttcgc ccactcaatc tgtctatcaa cagctaataa aagcaattct
    29101 ttagatattc tggcattcct aggtttagga ttc
//
LOCUS       DAZGOB010000052        29036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGOB010000052 DAZGOB010000000
VERSION     DAZGOB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.052"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(131..2398)
                     /gene="adiA"
                     /locus_tag="ACHLIF_002468"
     CDS             complement(131..2398)
                     /gene="adiA"
                     /locus_tag="ACHLIF_002468"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO9606468.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2597..3505)
                     /gene="melR"
                     /locus_tag="ACHLIF_002469"
     CDS             complement(2597..3505)
                     /gene="melR"
                     /locus_tag="ACHLIF_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO9606469.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCKSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRAMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3788..5143
                     /gene="melA"
                     /locus_tag="ACHLIF_002470"
     CDS             3788..5143
                     /gene="melA"
                     /locus_tag="ACHLIF_002470"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO9606470.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5246..6667
                     /gene="melB"
                     /locus_tag="ACHLIF_002471"
     CDS             5246..6667
                     /gene="melB"
                     /locus_tag="ACHLIF_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO9606471.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6806..7435)
                     /gene="yjdF"
                     /locus_tag="ACHLIF_002472"
     CDS             complement(6806..7435)
                     /gene="yjdF"
                     /locus_tag="ACHLIF_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO9606472.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7558..9204)
                     /gene="fumB"
                     /locus_tag="ACHLIF_002473"
     CDS             complement(7558..9204)
                     /gene="fumB"
                     /locus_tag="ACHLIF_002473"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO9606473.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9282..10622)
                     /gene="dcuB"
                     /locus_tag="ACHLIF_002474"
     CDS             complement(9282..10622)
                     /gene="dcuB"
                     /locus_tag="ACHLIF_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO9606474.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11193..11912)
                     /gene="dcuR"
                     /locus_tag="ACHLIF_002475"
     CDS             complement(11193..11912)
                     /gene="dcuR"
                     /locus_tag="ACHLIF_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO9606475.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11909..13540)
                     /gene="dcuS"
                     /locus_tag="ACHLIF_002476"
     CDS             complement(11909..13540)
                     /gene="dcuS"
                     /locus_tag="ACHLIF_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO9606476.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13721..13951
                     /gene="yjdI"
                     /locus_tag="ACHLIF_002477"
     CDS             13721..13951
                     /gene="yjdI"
                     /locus_tag="ACHLIF_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO9606477.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13963..14235
                     /gene="yjdJ"
                     /locus_tag="ACHLIF_002478"
     CDS             13963..14235
                     /gene="yjdJ"
                     /locus_tag="ACHLIF_002478"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9606478.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14462..14758
                     /gene="ghoS"
                     /locus_tag="ACHLIF_002479"
     CDS             14462..14758
                     /gene="ghoS"
                     /locus_tag="ACHLIF_002479"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO9606479.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14786..14959
                     /gene="ghoT"
                     /locus_tag="ACHLIF_002480"
     CDS             14786..14959
                     /gene="ghoT"
                     /locus_tag="ACHLIF_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO9606480.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15078..16595)
                     /gene="lysS"
                     /locus_tag="ACHLIF_002481"
     CDS             complement(15078..16595)
                     /gene="lysS"
                     /locus_tag="ACHLIF_002481"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO9606481.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16832..18289)
                     /gene="dtpC"
                     /locus_tag="ACHLIF_002482"
     CDS             complement(16832..18289)
                     /gene="dtpC"
                     /locus_tag="ACHLIF_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO9606482.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(18348..20495)
                     /gene="cadA"
                     /locus_tag="ACHLIF_002483"
     CDS             complement(18348..20495)
                     /gene="cadA"
                     /locus_tag="ACHLIF_002483"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO9606483.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20575..21909)
                     /gene="cadB"
                     /locus_tag="ACHLIF_002484"
     CDS             complement(20575..21909)
                     /gene="cadB"
                     /locus_tag="ACHLIF_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO9606484.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(22275..23813)
                     /gene="cadC"
                     /locus_tag="ACHLIF_002485"
     CDS             complement(22275..23813)
                     /gene="cadC"
                     /locus_tag="ACHLIF_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO9606485.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(24073..24187)
                     /locus_tag="ACHLIF_002486"
                     /pseudo
     CDS             complement(24073..24187)
                     /locus_tag="ACHLIF_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(24562..24711)
                     /locus_tag="ACHLIF_002487"
     CDS             complement(24562..24711)
                     /locus_tag="ACHLIF_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9606486.1"
                     /translation="MLIAWLQDNIDCESGIIFDNDEDKTDSAALLPCIEQAREARYFP
                     SVLSA"
     gene            complement(24711..25201)
                     /locus_tag="ACHLIF_002488"
                     /pseudo
     CDS             complement(24711..25201)
                     /locus_tag="ACHLIF_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            complement(25293..25868)
                     /locus_tag="ACHLIF_002489"
                     /pseudo
     CDS             complement(25293..25868)
                     /locus_tag="ACHLIF_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
     gene            complement(26074..26892)
                     /locus_tag="ACHLIF_002490"
     CDS             complement(26074..26892)
                     /locus_tag="ACHLIF_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO9606487.1"
                     /translation="MKRILFIAFFIAMFVTPIALILFVFDYGYKKSPQEYSLSFIKQL
                     PNTVNLSSLTYNKEDDFLYATQNSPAQLLKIARSGDIIGRAPLPFISDAETIEHIQGN
                     IFAAVDEKTSELFFFTVTKDMHISLRNKIQLEKFNKKNRGFEGLAWKAEGRMLFVAKE
                     RRPTGMFAYQLSPDLLSAKQVTIPEELNDIHVKDISGLDFNNESLMILSDESRKLLKF
                     NLTEMSFVEMMDLTKGNHSLTSDLLQPEGIVTLSDESIYVASEPDILAKFVPNK"
     gene            complement(27039..28184)
                     /locus_tag="ACHLIF_002491"
     CDS             complement(27039..28184)
                     /locus_tag="ACHLIF_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HFO9606488.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAKQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
     gene            complement(28857..>29036)
                     /locus_tag="ACHLIF_002492"
     CDS             complement(28857..>29036)
                     /locus_tag="ACHLIF_002492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606489.1"
                     /translation="KWNNYSTKTFRYIAITCSPDIRSRYTSTPYSPVLTGSGGGIKRN
                     KEQMAEQATMFVPPA"
BASE COUNT         7517 a         6645 c         6994 g         7880 t
ORIGIN      
        1 ggtggggccg cgcaaaaagt atgacatttt tgtttgcagc aatttttatg aaaacgttgc
       61 catactcaaa aggcctaagg ccaggcggcg taatgttatt taaacaatta cgccttcagc
      121 ggaatagtgg ttacgctttc acgcacataa cgtggtaaat accgtcaata atttcagtcc
      181 cttcagtttc gtgttcaaat ccagggaaat ggtggtccca ggattgcagc gagcgtaaat
      241 aacttacttg cggactgttt ttatcgccga agttttcacc agacagcagc atcgggattc
      301 ctggcggata cgggataact gagtttgccg cgatgcgtcc tggcagattt tcaatggata
      361 ccagttcgac attgttgtcg acaatcgcgt tgtacgcttc acgcggggtg atttccgcca
      421 tcggcaggcc ggaataggct tcgttcaacc gtgcgccagg gttgttttct ttcagccagg
      481 caaacatggt gtcacccaga tcgtgaatcc ccatgttcgc gtaagtgtca ggatattgtt
      541 caacaagttc cggcatcacc tgcgccagcg gtgtgttggc gtcatagtgg cgtttgaagg
      601 aacaaagggt gttaaccaga gtcccccatt tcccacgggt tacgcccata gagaacagga
      661 acataatttg gaagtcagtg gtgcgggtag gcacaatgcc gtggcgacca agccaggcag
      721 tgaccagcgc cgccggaaca ccggtttctt ccagttcacc atcttcaccc attcccggag
      781 caaggatgct gactttaatt gggtcgagca tactccagtt atccggaata tctttgaagc
      841 cgtgccagct ttcgcccgga tgcattaccc agcagtcctg aacggtggtc agcagtttgg
      901 ttggtgcgtc agcaaagtca taggttttgc cggtttgtgg gtcggtgacg acttctttgt
      961 tccacggttt gaagaaccag ctaccgtcag cggtgaactc tttatatagc cgcgccatcg
     1021 cctgacggaa atcaaccgct tcgtcaatca cttcctgtgt cagtgacagg ccgctgttgc
     1081 cgtccatcat cgacaccgcc acgtcgttgg atgcgcagat ggcatacagc ggggaggtgg
     1141 tggcatgcat catgtaggcc tggttgaagc gggagaagtt aatcgcccca cgaccttcac
     1201 gtacatgaat ataagaagcc tgtgacagcg cattcagcag tttgtgggtg gagtgggtgg
     1261 cgaaaacggt aggaccgttg tgatcgccag gttcgccgcg catggcatag tgatcggcat
     1321 agatcgggtt gaaacgtgca tagccgtacc aggcttcgtc aaagtgcaga cgatcggagg
     1381 ttttttccag cagatcctgc gcttctttag cgttataaca cacgccgtca taggtgcagt
     1441 tggtcaccac gcagtaagac ggtttttgcc cggctttgtc tttggtcagc gggctttcac
     1501 tgattttctt ctgcaaggtt tcaggttgca tttcctgcgg atagattggc ccgataatgc
     1561 cgtagcggtt gcggcttggc accatataga ccggtttcgc gcctgtcagc atcaagcctt
     1621 gttcgatgga tttatggcaa ttacggtcaa cgaccacgac atcgttatcg gtcatgcaag
     1681 cctgcatgat ggtgcggtta gagccggaag taccgacgac taccgaccag gagcgatcgg
     1741 caccaaatac gcgtgcggca tatttttcgc tttcgccaaa tgcgccagta tggtcaagca
     1801 aagaaccgag ggaagttcgt tcgatgccca tgtcggtgcg gaacagattt tcaccatagt
     1861 agtcatggta gaaacgtccg gcgggtgttt tggtaaaacc aacgccgccc tggtggcctg
     1921 gcgctgccca ggaatattca tggatgtcac tatatttcat cagcgcgctg aacagtggcg
     1981 gcaacagctg ctggcggtag cgggtcatcg cggcaacggc gcgtccggcg ataaagtcgg
     2041 cggtatcttc cagaatccag gcgaattcat cgacaagctc cagcagatcc cggtccattg
     2101 cggcgagggc tttttcccga tcgcccaaca ggaagaccgg cacgttttgt tggcgctcat
     2161 gaagcttacc gatcaattgt ctgacgtttt gatgttcgtc cggatgttcc atttgatagc
     2221 tgaacatcag gcagtcaatg gcttcgtttg aagagagaat ggcaaaacca tcatcaaagg
     2281 aggtggattt aatcacggta acattttgct ggcttaaagc atctgccaga cgctcaacgg
     2341 cgttaccgac ccaggtgtct tgatggagaa actcgctttc aacaattaat actttcatca
     2401 ttgcttaccc ggttatgaag gaatcttcgt tgcgggcaag tatcttccgg ccttttcggg
     2461 ctgtaaagcg cgtgaaaaac aaacattttt gaaatattta gttttataaa caatacgtta
     2521 tgtgattatt ttaaattatt ttcactgctt attatcgacg gctaaactat ttttttggct
     2581 gatactgata tcgtctttag ccgggaaacg tctggcggcg ctgttggcta agtttgcggt
     2641 attgttgcgg cgacatgccg acatatttgc cgaacgtgct gtaaaaacga ctacttgaac
     2701 gaaagcctgc cgtcagggca atatcgagaa tacttttatc ggtatcgctc agtaacgcgc
     2761 gaacgtggtt gatgcgcatc gcggtaatgt actgtttcat cgtcaattgc atggcccgct
     2821 ggaatatccc cattgcatag ttggcgttaa gtttgacgtg ctcagccaca tcgttgatgg
     2881 tcagcgcctg atcatagttt tcggcaataa agcccagcat ctggctaaca taaaattgcg
     2941 catggcgcga gacgctgttt ttgtgtgtgc gcgaggtttt attgaccaga atcggttccc
     3001 agccagagag gctaaatcgc ttgagcatca ggccaatttc atcaatggcg agctggcgaa
     3061 tttgctcgtt cggactgttt aattcctgct gccagcggcg cacttcaaac gggctaagtt
     3121 gctgtgtggc cagtgatttg atcaccatgc cgtgagtgac gtggttaatc aggtctttat
     3181 ccagcggcca ggagagaaac agatgcatcg gcagattaaa aatcgccatg ctcttacagg
     3241 ttccggtatc tgttagttgg tgcggtgtac aggcccagaa cagcgtgata tgaccctgat
     3301 tgatattcac tttttcattg ttgatcaggt attccacatc gccatcgaaa ggcacattca
     3361 cttcgacctg accatgccag tggctggtgg gcatgatatg cggtgcgcga aactcaatct
     3421 ccatccgctg gtattccgaa tacagcgaca gcgggctgcg ggtctgtttt tcgtcgctgc
     3481 tgcacataaa cgtatctgta ttcatggatg gctctctttc ctggaatatc agaattatgg
     3541 caggagtgag ggaggatgac tgcgagtggg agcacggttt tcaccctctt cccagagagg
     3601 cgaggggagc gtccgagcat catgaggccg aaaactctgc ttttcaggta acttattacc
     3661 ataaactcag atttactgct gcttcacgca ggatctgagt ttatgggaat gctcaacctg
     3721 gaagccggag gttttctgca gattcgcctg ccatgatgaa gttattcaag caagccagga
     3781 gatctgcatg atgactgcac ccaaaattac atttatcggc gctggttcga cgattttcgt
     3841 taaaaatatt cttggtgatg tgttccatcg cgaggcgctg aaaacggcgc atattgccct
     3901 gatggacatt gatcccaccc gcctggaaga gtcgcatatt gtggtgcgta agcttatgga
     3961 ttcagcaggg gccagcggca aaatcacctg ccacacccaa cagaaagaag ccttacatga
     4021 tgccgatttt gtcgtggtgg catttcagat tggcggttat gaaccttgca cggtgactga
     4081 tttcgaggtc tgtaagcggc atggtctgga acaaaccatt gccgatacgt tggggccggg
     4141 cggtattatg cgcgcgctac gtaccattcc gcatctgtgg caaatttgcg aggacatgac
     4201 ggaagtctgc cccgatgcca ccatgctcaa ctatgttaac ccaatggcga tgaatacctg
     4261 ggcgatgtat gcccgctatc cgcatatcaa acaggtcggg ctgtgccatt cggtgcaggg
     4321 aacggcggaa gagctggcgc gtgacctcaa tatcgaccca gctacgctgc gttaccgttg
     4381 cgcaggtatc aaccatatgg cgttttacct ggagctggag cgcaaaaccg ccgacggcag
     4441 ttatgtgaat ctctacccgg aactgctggc ggcttatgaa gcagggcagg caccgaagcc
     4501 gaatattcat ggcaatactc gctgccagaa tattgtgcgc tacgaaatgt tcaaaaagct
     4561 gggctacttc gtcacggaat cgtcagaaca ttttgctgag tacacaccgt ggtttattaa
     4621 gccaggtcgt gaggatttga ttgagcgtta taaagtaccg ctggatgagt acccgaaacg
     4681 ctgcgtcgag cagctggcga actggcataa agagctggag gagtataaaa acgcctcccg
     4741 gattgatatt aaaccgtcac gggaatatgc cagcacaatc atgaacgcta tctggactgg
     4801 cgagccgagt gtgatttacg gcaacgtccg taacgatggt ttgattgata acctgccaca
     4861 aggatgttgc gtggaagtag cctgtctggt tgatgctaat ggcattcagc cgaccaaagt
     4921 cggtacgcta ccttcgcatc tggccgccct gatgcaaacc aacatcaacg tacagacgct
     4981 gctgaccgaa gctattctta cggaaaatcg cgaccgtgtt taccacgccg cgatgatgga
     5041 cccgcatact gctgccgtgc tgggcattga tgaaatatat gctcttgttg acgacctgat
     5101 tgccgcccac ggcgactggc tgccaggctg gttgcaccgt taaaacgcga ctaaacgcta
     5161 ctgcgccggg ggatttattc cggcgcacac ctctgacgat accaataaca gaaggcgggc
     5221 gttggtaaca gcgacccgat accctatgag catttcaatg actacaaaac tcagttatgg
     5281 atttggagcg ttcgggaagg attttgcgat cggcattgtg tatatgtacc tcatgtatta
     5341 ctacaccgat gtcgttgggc tgtctgtggg tttggtcggt actttgtttc tggtggcgag
     5401 gatctgggat gctattaacg atccgattat gggatggatt gtaaatgcta cgcgatcgcg
     5461 atggggtaag ttcaaaccct ggatcctgat cggtacgttg gcaaactctg taatcttatt
     5521 tctcctcttt agtgcgcatc tgtttgaagg tactactcag attgtctttg tttgcgtgac
     5581 ctacatcctc tggggcatga cttacaccat tatggatatt cccttctggt cgctggttcc
     5641 aaccatcacg ctcgataaac gtgagcgcga acaactggtt ccttatccgc gtttttttgc
     5701 cagtctggca ggctttgtta cggcaggtgt gacgctacca tttgttaatt atgtcggcgg
     5761 tggcgatcgg ggatttggct ttcagatgtt cactctggta ctaatcgcct tttttattgt
     5821 ttcaaccatc atcactctgc gcaatgtgca tgaagtcttt tcgtcagaca atcaaccgtc
     5881 tgctgaagga agccatctga cacttaaagc catcgttgcg ctaatttata aaaacgatca
     5941 gctttcatgc ctcttgggta tggctcttgc ttataatgta gccagcaaca ttattaccgg
     6001 atttgctatc tattatttct catatgttat cggtgatgcg gatttgttcc cctattatct
     6061 gtcgtatgcg ggagctgcta acctggtgac gttagtattc ttcccacgct tagttaaatc
     6121 attatcccga cgcattttat gggccggagc atctattctt ccggtgttaa gctgtggtgt
     6181 tctcctgtta atggcattaa tgagctatca caacgtcgtc ctcattgtga ttgcgggtat
     6241 tttgctgaat gtgggaacgg cgcttttctg ggtattacag gtcatcatgg tggcagatac
     6301 cgttgattac ggtgaatata aactgcacgt acgctgtgaa agtatcgctt actccgtgca
     6361 gactatggtg gtgaagggcg gttcagcctt tgcggctttt ttcattgcgg ttgtgttagg
     6421 gatgattggc tatgtaccga atgttgaaca gtctacgcaa gccctattag gtatgcagtt
     6481 tattatgatt gctctaccaa ctctgttttt catggtaacg ctgattctct acttccgttt
     6541 ctatcgcctc aatggcgaca cgctgcgcag gatccagatt catctgctgg ataaatatcg
     6601 caaagtaccg cccgagcctg ttcatgctga tattccggtc ggtgcagtga gtgatgtgaa
     6661 agcctgacgt ggacaatagc taacgaaaat ggcctgatgc gatatgttta tcaggccacg
     6721 tcttaatgta atgctttgga tttgcaaatt ttgtagaccg gataaggaat tcacgccgca
     6781 tccggcatca acaaagcgca cgttgttatc cggttatcaa gccaaagcgc cgtagctggc
     6841 ggcaatgaaa gcgagcgagg aatatcaccg tcgttaatgc gccaagcagc gcgcagaaca
     6901 tatcggattg cgtgtcccac tggtcgccct gggtgccgag aaaatcatcc gctccctggc
     6961 ccattgccag cgccgcccac cactcgatta attcatacat cgcgcttatc gccagcgcca
     7021 cgcagcagac caggaacgcc accattttac gtccgcgcac gtacatcccg cgcacgagaa
     7081 tttctcgtgc caccagtgca ggcaccagcc cctggaaaaa atgccccagc ttgtcatacg
     7141 gattacggct caaccccagc cattcctgca cctcaaaacc gatggggact ttcgcgtagg
     7201 tgtattgccc gccgaccatc aggatgatgg cgtgaaagaa aatgagcgta tagagcagcg
     7261 gcgttaacgg ataacgttta gcggtggcaa gcagtagcgg cacgacaata atcaccggtg
     7321 tcacttccat cagccaggtg agtttgtcat gggccgaaat gccggtataa atcaggatta
     7381 acgttagcgc cagtgcgccg gtgttaagaa ttaacggctt gagtgtacgg gtcatggtga
     7441 gttcgcaagt cagggaaaat actgactatt caccagcaag cgtgaaatta caatcgcaaa
     7501 agaggtggcc cagggggatc acctggcagc atgctgccag gcgctgggcc gaagaggtta
     7561 cttagtgcag ttcgcgcact gtttgttgac gatttgctgg aagaagtcgt tacctttgtc
     7621 atcgaccagg ataaacgccg ggaaatcttc tacttcgatt ttccagatag cttccatacc
     7681 cagctccgga taagcgacgc actccagatg cttgatgctc tgctgcgcca gtaccgccgc
     7741 cggaccgccg atgctaccga ggtagaagcc gccgtgttta tgacacgcgt cggtaacctg
     7801 ctgactgcgg ttacctttcg ccagcatgat catgctgccg ccgtgggatt gcagcagatc
     7861 cacgtaggag tccatacggc ctgcggtggt tgggccaagt gaacctgatg gataaccggc
     7921 aggggttttc gccggacccg cgtagtagat cgggtgatct ttgatgtact gcggaagttc
     7981 tttaccggcg tcaatcagct ctttcagctt ggcgtgtgca atatctcggc ccacgataat
     8041 ggtgccggtg agcgacaaac gagtggatac cgggtattgc gaaagctggg cgaggatctc
     8101 tttcatcggg cggttaaggt caactttcac cgcttcgcct tcaccggcct ggcgcagttc
     8161 ttgtggaatg tactggcctg ggttgtgttc cagtttttcg atccagatac cttcgcggtt
     8221 gattttcgct ttaatgttac ggtcagcgga gcaggagacg cccatgccga ccgggcagga
     8281 tgcgccgtga cgtggcagac ggataacgcg aatgtcgtgc gcgaagtatt taccgccaaa
     8341 ctgcgcgcca agaccgagtt tctgggcctc ttccagcagt tcctgttcca gctggacatc
     8401 gcggaacgcc tgaccatgtt cgttcccttc cgtcggcagt tcatcgtaat agtgagcgct
     8461 tgctaacttg acggttttca ggttggtttc cgcagacgta ccgccaatca caaacgcgat
     8521 atggtacggc gggcaggctg cagtaccgag ggtacgcatt ttctcgacga ggaagttttt
     8581 cagtttgccg ggagtcagca gggctttggt ttcctggtag agatacgttt tgttggcaga
     8641 gccgccgcct ttcgcaacgc aaaggaattt gtactcatcg ccatctaccg cgtacaggtc
     8701 gatttgcgca ggcaggttag tgccggtgtt gacctctttg tacatgtcca gcgccgcatt
     8761 ctgtgaatag cgcaggttat cttcgatata ggtgttatag acgcctttcg acagcgcttc
     8821 ttcatcaccg ccaccggtcc acacgcgctg gcctttttta ccgacgatga tcgcggtgcc
     8881 ggtatcctgg caggtcggca gcacgccttt ggcggcgatt tcggagtttc ttaagaattg
     8941 cagcgccacg tacttgtcgt tttcgctggc ttctggatcg tgaagaatag ccgcaacctg
     9001 tttctggtgt gccggacgga gcatgaaaga agcgtcgtga aaggcttgct gcgccagtag
     9061 ggtcagggct tctggttcca ctttcaggat ggtttcgccg tcgaagtcgg caacgctaac
     9121 gtaatcggaa gtgagtagat agtattcggt attgtctttc cccatcggga aaggtgcctg
     9181 gtagataaag ggtttgtttg acatagcttc cagcctgtaa ctctgtattt gttattcgaa
     9241 aatggcgtgc cgcgtagtca cgggcggcac gcaaagtgca tttataagaa cccgtacatc
     9301 gcggcgaaga tccagccgaa gacgcacgat acgctcacac caatcaaccc cggcagaata
     9361 aaactgtggt tgatgacgaa gcgaccgatg tgggtggtgc cggaacggtc aaactgaatc
     9421 gctgccagat cgctcggata agtcggcagg atgtaataac cgtagcaagc cggtgctgaa
     9481 gccacgatgt atgccggatc aacgccgatc gccagcgcga ccggaacaat cgccgccagc
     9541 gccgcagcct gagagtttac aaacttggaa accagcagca gaacaatggc atacgcccac
     9601 ggatactctt tcaccatttc acccagtacg ccctgaattt cagacatatg cgcaccgaac
     9661 atggtttctg ccatccatgc gataccgtac accgccacga tggcgatcat accggaacgg
     9721 aagacttcgt tttttgagat agacgcggga ttggttttgg tcaggataat aatcagcgcc
     9781 ccggtcagca gcataaacat ctgaataacc agtaccatcg acagcggttt gccgccgaag
     9841 gatggacgca ggtccgaatc agcaccaaga agggcgacta cagcgattgc accgaggaaa
     9901 atccacattg ccagccagtt gcttttcggc agttttttat cgagcagcgt cgcggtatca
     9961 ccgtaaacat actcacggtt ttccggtacg gagatgaatt tctggaactc ttcgtcttta
    10021 tccagatctt taccgcggaa ccagctgaag ataccgatcg ccaggatacc gattaacgtc
    10081 gatggaatgg tgattgccag caggtcgagg aactcaagat ggcgaccatc aaaggtgaca
    10141 ttacccagca ttgcaaccag agacacgacc gcaaccgaca ccggactggc gataatcccc
    10201 atctgtgcac cgatagaact tgccgccatc ggacgttccg gacggatgtt gttcttaatg
    10261 gcgacgtcgt agatgatcgg cagaatggtg taaaccacat gacccgtacc gcaaagaatg
    10321 gtcagtgtac aggtcacaaa cggcgcgaca attgagacat atttcgggtt gcggcgcagc
    10381 agcttctcgg caatttgcag catgacatca agaccgcccg aagcttgcaa ggtcgccgat
    10441 gccgccacca ccgcaatgat aaccagcatg acatcaaccg gtggtttacc tggctgaagg
    10501 tggaagacga agaccagaat gaccagaccg ataccgccta ataaacccag cgcgatacca
    10561 ccctttctgg caccataaaa cagacatatc agtattatga taagttggat agtaaataac
    10621 atgtgtgaac cctcgcgata atcctattta aatttttgct gaatagatca cagtcacgtt
    10681 ctgttttgta tgaactgttt tcagagcctg actaaatatc cgtttggtcg ttaccggctt
    10741 tagcaaatac ctcacagtaa atattggctg attaaagcag cggcaaattt atgcactgtg
    10801 ataagcggct ttttcagaga gaaaaactct ctggcgatgg gtttatagtt tgtactgcgc
    10861 agatgttaat gttttgtttg ctgttatctt attgatattt atcggtctat tttttgttct
    10921 cagggcagat atcattaggt ttaaagatta atctgatcta cccatttgtg ggtaaaaata
    10981 cacataatgc gggtgacata atagttaatt aacttttgtt atcgttttga aattaaaaac
    11041 accgttcacc tgaagagata ttaattttta gcgatgatgg agggataatt atatttgatc
    11101 tggcacaagt tttactgatg aaggatgtaa cttgtgccag gggtattttg cattacggta
    11161 ataattatta cttacagata accagcgatc cgttattggc aatattgttt cagtagtgag
    11221 tagtgttctg cctgaatacg gtaacggtaa accggacgcc ccgtgacgcc ataatggata
    11281 ctggtgaaca agatgtggca gttgaccagc cagatgaggt atttacggca ggaaacacgc
    11341 gagatgttaa cctcgttagc cagctcgtcg gttgaaaact catagtcctg atgcgcgtca
    11401 atccactggc acagcgtgcg taaagtctgc ggcgttaagc cttttggcaa gcgacgagga
    11461 tcctgttcgt tggagctgct gccgtggatt agctgatcaa gctcggcctg gtcataatac
    11521 tgatgttttt ccagcgccat tttcttttgc cgccagccgg tgagcgcctc ttcaaagcgg
    11581 gaagcctgga agggtttgat caggtaatcc acgacaccgt aatgcagcga atctttaatg
    11641 gttgccgcat cggctgcgga ggagatgaca atcacatcac ttttgcaacg cgcgttatgc
    11701 aggacaggca gtaaatcgag cccgttctct ttttgcatat agatatcgag caatatcagg
    11761 tcgataggcg tatcgctatt gaagataatc tctttggctt tctccagcgt cgaggctgtt
    11821 ccacagcatt gaaagcctgg gatttgtgct acgtatcggc gattcagctc cgcgaccatt
    11881 gcgtcgtcat cgataattaa tacattgatc atctgttcga cctctccccg tcccagggta
    11941 tctggacaaa aaattgtgtg aaaatcccgg gttccgattc cacggcgatg ctgccgccga
    12001 gattttctac ctgttgtttg acaagtgcta aaccgacgcc tcgctcgctt ccttttgtcg
    12061 agacaccttt gtcaaaaatg tgatcgattt tgtcgggtgc gattcccggt ccatcatcgt
    12121 taacttcgca gtgcagccag ccgtgacggt agtgcaatgt tacgctaatt tcgccaccgg
    12181 gttccggccc taatgcttcc agcgcgtttt ctatcagatt tcccaatgtg gtaatcagcg
    12241 tcgcgacctg gtcctcactg ccgctgtctg gcagctggct ttcactgttt aaaatcagcg
    12301 tatggcctaa atcggtcgcg cggttaatct tgctgattaa aaaaccagcg ataaccggag
    12361 atttgatctt acccagcaga gagccaatct cttcctgata gttattggct gttttgagaa
    12421 tgtaatcttc caactgctta taactcttca gatgcaataa tccgagaatc acatgcaatt
    12481 tattcataaa ttcgtgggat cgttcacgaa gtgcatcagc atagttgacc agaccgtcga
    12541 gtcgctgcat cagtttacgt acttcagttt tgtccctgaa ggttgaaatg gcaccgatga
    12601 taacgccatt actgcgcacc ggaacggtgt tgatcagtaa tagccggtct ttaatcgtaa
    12661 tctcttcgtc gcggcgcggc gtaccgtcgc gtaacacttc cgagacatct accacctgtg
    12721 accatgagtg gctgagcgtc gacagtttct catcgtcctg cgacttacgg taattcagca
    12781 attcttgtgc ggcatcgttg atcagcgtga cctcgccgcg atcgtccacg gcaacgacgc
    12841 cttctttgat agactgcaac atggcctggc gctgctcaaa cagcgtggag atttcgtagg
    12901 gttctaggcc gaaaaggatt tttttcagta ccttaaccag aatgcaggtg ccaatcagtc
    12961 cgaccagcat gccaaataat accgaccaga taatgctcca gcgactgtca ttgatttgtt
    13021 gggtcacgcg gcttaactca aggccgatcg ccaccacgcc aatttgttta tgattttcat
    13081 cgtagatggg ggtaaatacg cgtaaagcct gcgccagaaa accgcgattg atagcgacat
    13141 tttctttgcc attcagcgct ttaaggatgt catcaccttt aaatggctga ccaatacgct
    13201 gggcttcagg atgcgagtag cgaagacttt gcatatcggt aacgacaata aacagcagat
    13261 cgttgcgttt gcgcacggct tccgcgatgg cctggattcc gctctcctgc ggttttttct
    13321 gcaagccctg acggatttcc ggcgagtcgg cgagggtacg cgccactgcc agtgccttgt
    13381 tggccagccc gtctcgcgtc atatcactga tttgcgagaa gtaaatcaga tgcaccacca
    13441 atagcaccga gaacagtacc gcgctgacca ttaagatcac tgtggtactc aatttcatcg
    13501 gacgtttgcg taacatgcgg tagggcaatg aatgtctcat cagcttcctt gtgtgacaaa
    13561 tttcttaagc attatctctg atgaggcggg taattcaaag ggagtaagaa tgattggcta
    13621 tataggggaa gagactctgg caacggaaac tgccagtgct gtatgaagat tccagggcta
    13681 tgcttatagc gataatcata ctgatgagag agggaaagtc atggatcagg cgctactgga
    13741 cgagggttat cgctgttata ccggcgaaaa gatcgatgtc tatttcaaca ctgcgatatg
    13801 tcagcattct ggcaattgcg tacgtggcaa cggcaagtta tttaatctca aacgaaagcc
    13861 gtggatcatg ccggatgaag tcgacgtcgt cactgtggtt aaagtgattg atacgtgccc
    13921 gagcggcgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat acgcgaaggc
    13981 cacaataaat tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg
    14041 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
    14101 caggggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
    14161 aaaattatcc cactatgccc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
    14221 gatattcgca gttgatggga gagtacagag tcacgatatt tttcatgctc tccgcggagt
    14281 gatgcaggag agcatctgaa gggtaggggg atgcacaaag aatgggcaga gagcgcgttt
    14341 ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc
    14401 atcaatcggg gttaataatt tttgcgtccc cagagcattc aatattgata ggagtcatat
    14461 tatggaaggt aaaaacaagt tcaatactta tgttgtttct tttgattatc catcatctta
    14521 ttcctcagtg ttcttaaggt taagatcatt gatgtatgat atgaatttct cttctatcgt
    14581 ggctgatgaa tatgggatac cacgacaatt gaatgaaaat tccttcgcaa taacgacatc
    14641 gttagccgca agtgaaatcg aagatttaat caggcttaaa tgcttagact taccggatat
    14701 tgattttgac ctcaacatta tgacagttga tgactatttc cgtcagtttt acaagtagcc
    14761 aggaaggata agaagaacat aaaaaatggc actattctct aaaatattaa ttttttatgt
    14821 gattggtgtg aacatatcct ttgtcattat ctggtttatc tcacatgaga aaacacatat
    14881 tcgtttactt agtgcattcc tggtcggaat aacctggcca atgagtctgc ctgtggcatt
    14941 acttttttct ctcttttagg agctgtagtt gagtggaccc gctggttatc gtgaaacgaa
    15001 gcgtctctcc tcaaaactgg atttgcggaa cgacgaacga agcagtcgcc tgactgcttc
    15061 gttcattaaa gtgaaattta tttctgtggg cgcatcgccg ggaagagaat aacgtcgcga
    15121 atagtatgac tgtttgtaaa cagcataatc attcggtcga taccaatacc cagaccagcg
    15181 gttggcggca gaccatattc cagcgcagtc acgtaatctt cgtcatagaa catggcttcg
    15241 tcgtcacctg cagctttagc gttaacctgt tcctggaaac gttcagcctg atcttctgcg
    15301 tcgtttaatt cgctaaaacc attaccgatt tcacgaccac cgatgaagaa ttcaaaacgg
    15361 tcggtgattt ctgggttaac atcattacgg cgtgccagcg gtgacacttc tgccggatat
    15421 tccgtaataa aggttggctg aatcagatgt gcttctgcca cttcatcaaa gatctccgtg
    15481 acaatacgtc ccagccccca gcttttctct accgtaatac cgatagattc agctaatgct
    15541 ttagcagcat caaaattatc caggtcggcc atatcggttt ctggacgata ttttttgatt
    15601 gcttcgcgca tggtgagttt ttcaaacggt ttgccgaaat caaacacatg ctcgccataa
    15661 gtgactttag tggtacccag aacctcttgt gccagggtgc ggaacaggga ctctgtcagt
    15721 tcaatcaaat cgtggtaatc cgcatacgcc atgtagagtt ccatcattgt gaactcagga
    15781 ttatggcgaa cagaaatacc ttcattacgg aagttacggt tgatttcgaa tacccgttca
    15841 aaaccgccta caaccagacg tttcagatac agctccggcg cgatacgcag gtacatatct
    15901 aaatccagag cgttatgatg ggtaataaac gggcgagcag atgccccacc tggaattacc
    15961 tgcatcatcg gggtttctac ttccataaag ccgcgcgcga ccatgaactg gcggatagcg
    16021 gccagaattt ttgaacggac aacaaacgtt tgacgggatt tatcgttagc gatgaggtcc
    16081 agataacgtt gacgataacg gacttcctga tcctgcagac catggaattt atctggtaaa
    16141 ggacgtagtg cttttgtcag caggcgcaac tcagtacagt gaatggaaag ctcaccggtt
    16201 tgcgtcttaa acagcgtacc gcgggcaccg ataatgtcac ccagatccca ttttttaaac
    16261 tgatcgttat aaacaccttc tggcaggcta tctcttgcaa cgtacagttg aatacggcca
    16321 ccgacatcct gcaacgttac aaaggaggct ttccccatga tacgacgggt catcattcgg
    16381 ccagcaaccg agacttcaat gtttaaggat tccagttcct ggttatcctt cgcatcaaac
    16441 tcttcgtgca gctggtcaga ggtatggtcg cggcgaaaat cattgggaaa cgccacacct
    16501 tgctgacgta gtgccgccag tttttcgcgg cgatttctca gttcatcgtt aaaatcaata
    16561 gcctcattgg ctccccgtgt ttcttgttca gacattttgg ttcctctaaa tccagctttc
    16621 aatttagcgt agataaagag acagatcggt acactgtatt atctgcctcg actattaata
    16681 actcagttta tcttatcgaa actaacgctt acctcaagtt gatatcacta ataaataacc
    16741 atcaaaatgg tttaaatgac ttattaatct agatttgcta gtgttataaa ctgaaagtta
    16801 atttattcat aaccaattgt ttttacgaca gttaatcgtt gctctcctgt atcatattcg
    16861 tcggcgtgct aaacagaaaa cgggtagcaa aggccaatac gacgatgatc gcgacacaag
    16921 ccaacgtcca ctctcccatc tgagaaaaga atcgctggta agctgcaatt gccataccgc
    16981 taatttgcga ctctgtcgtc tgctgtgcca cgacgcctgc cagccagttg gcgaccgcgc
    17041 ctgttgccag catataaata ccggttaata cgccagacat tttcagacgc gtgatttgcg
    17101 caatcgccac cgggtcaata aagagttcgg caaagcccat tagcgccagc ccggatatca
    17161 tcacggccat tgacgcttga ccgccagccg ctgcatgtcg ggcatcaaat gccaacaaca
    17221 taaagccaca agccatcagc agtaagccaa aggcaaactt cagccagacg cgcaatgttg
    17281 agttgccgcg gctttctggc gacgccagcc aggccagtac aaccccagcg agcatcactg
    17341 caattgcatt caccgactgg aatagtgctg taggtacttc aatattgaat gcctgacgat
    17401 tcacaaagcg atcgataaac aagctgatgg tactgccgcc ctgttgtgcc agtacccaga
    17461 acaacgtccc gacaaacatc aacaatacaa tttgccaaag agcacggcgg tgttcgggga
    17521 atttgatcat catgcgggca atgatttgtg cggcaatgag gcaaacgatc gccagcaaat
    17581 atccggacca gtcgttctcc agcagcaggg taaaaaatac tggggctaaa cagagcatca
    17641 ccactaacca gctccatact ggtaaggcaa atttcacgct agtgagcgct tttttatcca
    17701 tactacgcgt ggactggaaa tgacgatgcc cgcttaagaa aatcaacaaa ccgataaaca
    17761 tgccgccacc cgcaagggca aagccaacat gccatccata ccactgagca gccaggccgc
    17821 aggcgattgg ggcggcgata gaaccgatat tgcccgcagc atacagcagc gaaaaaccgc
    17881 catcacgtcg atgatcgttc tcgtcgtaga gctcgccaag caaacagctg atgtttgatt
    17941 tgaataaacc gtagccacaa ataatgattg ccagcgccag atacaggcta aaggttgaat
    18001 ttgtatcaat gcccagcacc acatggccaa gggtcattaa cagcgcgccg gcaatcactg
    18061 cagtgcggtt gccgagcagg cggtcggcaa gccagccgcc gagaataggg gtaacgtaaa
    18121 ccagagaagc atatgcgctg aacaggctga tggcatggtt atcatcaaaa ccaagctgat
    18181 gggtgagata gagaatgagt aaggcacgca tgccgtaaaa actgaagtac tcccagattt
    18241 ggatcgccac gatatagtat atcgcgcgcg gctgtgaggg tgttttcatg tgttctcctt
    18301 atgagcaaaa aagggaagtg gcaagccact tcccttgtac gagctaatta ttttttgctt
    18361 tcttctttca ataccttaac ggtatagcgg ccatcagcct gacggtatgc accgtgaata
    18421 tcggtttcaa agcccggata gtgagcgccg atttcacaca gcatctgcag gaactccaga
    18481 accggacggc tttcttcggt gatcatttca cccggcatta ccagaggaac tcccggcgga
    18541 tacggaagga tcatattggc gttaatacga cctaccattt cgtcgaggta aacttcttcg
    18601 gtcataccgt gcagctcttt ctggaatgca gcatacggag tcattaccat cgtcggcagc
    18661 acttcaaatg cgcggtacat cagatccggc agattgtggt gaacaatcag tttgtggata
    18721 ttctgagcca gttcctgaat acgcatgttt tcatagaatt caggatcttc acgatacaga
    18781 gacggcagca tgtttttcac acgcaggttc aggtcgaacg cacgtttgaa gtcagtcaga
    18841 gcacgcagca ggctcagtgc tttggtctta tcgataccga tgctgaacag gaacagcagg
    18901 ttatacggac cggttttctc aacaacgatg ccatgttcgt cgaggtattt cgccacgatg
    18961 ctggccggaa taccaaagtc gctcatggtg ccgtcttttt ccatccccgg agtcagcagg
    19021 gtgactttga tcgggtcaag atacatgtgc tcgttatcga tgtttttgaa gccgtgccag
    19081 gtgctgtcag aacgcagcgg ccagcattca gtcgtatcga tatgatccgg ctgccataca
    19141 tcaaagaacc agccatcaga ttccgttctc agacgtttga tctctttacg gaatttgatc
    19201 gcacgttcaa tggaaccgtt gatcagacgt ttacctgcat tgcctttcat catcgccgca
    19261 gcggtttcag tggacgccac gataccgtag tgcggagaag tggtggtgtg catcatgtag
    19321 gcttcgttaa aggtttcttc gtttacgtca cctttaacgt ggatcatgga agcctgagag
    19381 aacgccgcca gcagtttgtg agtggactgg gtttcgtaaa tcactttccc ttctacacgg
    19441 ccaccgctca taccgcattt accttcgtaa atcggtgaga agttggtgta aggcacccac
    19501 gcggagtcaa agtggatgga tttcacatcc agtgttttct tgatgaagtc ggtgttgtac
    19561 agcagaccat cataggtaga gttggtaatt acagcatgta ccggccaggt tgcgtttggt
    19621 gtttctttca cgcgcttagc aatggtagcg tgctggaatt cactctgtgg gataccacca
    19681 agaataccgt aagcgttacg ggtcgggcgg aaatagattg gcgtaacatc gctcatcatc
    19741 atcaggtggg tcagcgattt gtggcagtta cggtcaatca gaatggtgct gcctgccgga
    19801 gcagagtaca tgccaacaat tttgttcgca gtggaagtac cgttggtcac catgtagctg
    19861 cggtctgcgt taaagacgcg agcgatatac tgttctgctt ctttgtgtgg accactgtga
    19921 tccagcagag aacccagttc agatactgaa atggaaatat cagatttcat ggtattcgga
    19981 ccaaagaaat catagaacag gctacctacc gggcttttct ggaatgcagt accgcccatg
    20041 tgaccaggag tacagaaagt atatttacct tcacgaacat atttaaacag tgctttagtc
    20101 agcggaggca gaatagtgtt gatatattcg tcagtggtct gcttgatctt attagcaata
    20161 tcttcagcag cacccagcgc atattcaaag aagctaatct gtaaacgcag gtcattcagg
    20221 cttacatcga gagtggaata cgtattagcg aacgcgtaca acggcaggtt ctcgttcatt
    20281 ttgctaattt cttcgcacag ctcgagatta tatttatccc agtcaaaaat aacgccgcac
    20341 agacgcgcat tgttttcgat cagttttaat aagtcgtcac ggtcgttcgg gtaaacaatc
    20401 tggaagttca gacgttcaag cgcgcgatga agttcacgga tgggttcttc tttaaaataa
    20461 acccccatgt gattcaatat tgcaataacg ttcatagtca tatctccagg taaaaaaggc
    20521 ccctcccaac acatgggaca aaatgaaagg aggagcctcg gaaaatactt ttaattaatg
    20581 tgcgttagac gcggtgtggt tatccattga gtggctctgg cgctcgtgca ttttgcgagc
    20641 gtagaacatc aggataatca ggctgacgat gaaggtacct gccagctcga aggagcttgc
    20701 gcccatcagc gcgatgaagc agaacacaca acccagtaca gagcagatca ggctgacaaa
    20761 gttgcggatg ttaacgcctt caaaacgaat caggtcaacg caagagtaga agtacggcag
    20821 catagtcagc agtactgcga taccggtcag ttcaccgaac aggtcagatg ctttaccacc
    20881 ggcagagttc atcagagtga tgaggatcat cagggcagtc attttcactg cagccagcag
    20941 cagacctttt ttcggaatac cgttgctgtc gacttcacca taaactttcg ggaagttacc
    21001 gtcgttagcg gcacgtacac ctgcctggcc taccaacatc atccaggagc ccagagaagt
    21061 caggcacgca aaggcggtga atgcagaaac cagcggcgca gcccagttac cgaggatggt
    21121 tgaagcactg attgcaaacg gagcaccgga agctgccatt acagaagatg gatacatacc
    21181 ggaaagcacc tgagtcgcag cgatgtaaac aatacctgct aaaccagtac ccagcatggt
    21241 tgccagcgga acggtacgtt tcgggttttt aaccatacca gtacttactg ctgcggattc
    21301 aacacccacg aaggcccaca ggcagagcag aatactttta atgatcgcat gaccatcagt
    21361 ggtatccgca gtattccagt tagctgcata agttgccgca tcaaaccaat gccagccaac
    21421 aatagcggtc attactaccg gaataagaac cagcaccaga ccaatagtgg ttaaacggct
    21481 tacccaagta ccgccgagca tatttacaaa ggtaaatacc cagacgatag caatacaggc
    21541 gatacccgcc ggaacaggat catttaatac tgggaagaag gtggaaagat aagataccgc
    21601 ggtaatacca atcgccaggt taccaatcca gttagcatgg taataaagaa cacctgtctg
    21661 aaaaccaaat gcaggggaaa tttctccggc ataagcaatt gggccacctt gttgcgggtt
    21721 ttttgttgcc agtcgggcat atacatacgc cagcgacatt gcaccaataa tagagataat
    21781 ccaaccccag atagcaatac caccgatact tgctaggttc gcaggtaata atgcaatacc
    21841 gctccccatc atattaccgg caacaacacc ggtacaggca aatagcccga tcttcttggc
    21901 agaactcatg ctcttctcct aatttcattt ttgaatttgg agtccgggtc atgatgtata
    21961 actatttcct gaccagacca aactggcgct aagaatactc acgaaaaaag gattaatcct
    22021 aaagattagg tgaaataaca caaaagtttc tgtaagtgag aacttgaggt tttttattaa
    22081 cacatcagga tcgcaagttg atatcatgaa aagataaaca ttcaatgttt acaatggatt
    22141 gcgggatatt ctctggttaa atttatgtaa taaaaattat gcggcaaata aattgccgca
    22201 acatattata ccaacagaaa catacaaaaa ctcaacaaca aatattcccg agcataaatc
    22261 aacccgggag ttacttattc tgaagcgaga aatttgtcga gataaggtac aacataagga
    22321 acagaagtct ggaatatacc attttcaatc cagtaaaggg tgtttgcccc tgggcgtaaa
    22381 ttaaaggcgg tgagatatgc atcagccgct tcccggttca tccccttcat ttcataaacc
    22441 ttgccaagca acacataatt tagccaggac atttcaagat caatgccagt atttatcgcc
    22501 tggtaagact catctgtttt accttttacc agagcactga ccgcttttat ttgatatata
    22561 atggacaggt tattcaattc cggcagtgta acaatgttgt ctatttctgt gttcagtgct
    22621 gctaattgtt tttcatctaa aggatgttga gaatggcgca cgatatcaac taatgctttt
    22681 tctgctctcg cgtaggtaaa ttctggggat gattgaacaa tctcacctaa taattcactg
    22741 gcacgattca atgatttatc atcgccatgc agtaaataat catgtgcctg ataaaaatta
    22801 gttaataacg caccacgatg aggcaatatt tgctggagcg tctcctgcat tcgttgtggc
    22861 cacggttggt ttaacgcttt tgataaactc tccagtaaat cattttgaat cgccagctga
    22921 ttaccgttag tgatgacata acgtttatcc agcatggttg aaccatctgc attgtctacc
    22981 aattttatcg acataaagca ttgttgagca cggtattggc gctgattaac aaacgcaata
    23041 gataatgttt taccggaact gctcggttca tcaatgttgt agttgatttt gtcatgcacc
    23101 ataaaggtgg agaaggtgtt aagtgatgtc gccaccaaat cacccacgcc tatcgcgtaa
    23161 gagagctgat acggggaact ccagctgtta caacttttat ttaccatatt aatgtcaata
    23221 tcgcgtggat tgagcaaaat acgcgatttg ctcataggaa gacgtgtatc aagagttgaa
    23281 aacgctacca gtgctacaca gatacctaac gacaacagga aaaaaaacca tacccaaaag
    23341 gtagtgaatc gtttgctttt aactggggat tgttcaggtg gcgttgcggt gttttgaatg
    23401 ttaagactgt gggagggaga atctgtggca ggaaccgcct ctggtatagg gggaggcgaa
    23461 gatagcatta tttcctctcc ctcttcttcg ctgtaccaga taaccggcac cattaattta
    23521 tagccgcgct ttggtacagt agcgatatag acaggactat cttcatcatt atcttttaat
    23581 gacttacgta gttctgagat actctgcgtc acaacatgat tggtgacaat acttctcttc
    23641 cagacattat cgataagttc atccctgcta agtacttcgc cactgtgttg agcaaagaaa
    23701 accagaagat cgattaatct cggctcaagg gtaagttgac gcccattgcg gctaatttgg
    23761 tttatggacg gagtaacaag ccattcgcca acgcgaacta caggttgttg cataatagaa
    23821 actcattcga aaagggaatg atgcaatgat aattgccaca acctattttt accatctata
    23881 gatgggttta tttacatatt attggtgaat gcaagacgtt atttttacca gccataaact
    23941 tcttgattac atagtattac gaaaggattt tactgagaac cagaagtaat attccttacc
    24001 atcaaaattc atcatctttg ccaaagaaaa atgttcagaa aataatccat ggaaaattgt
    24061 ccggagcgct tactatttta atggattgtt agtctttgca tgagcaagcg gactgataca
    24121 tttctctttg ttctcattca gaaaatctca tcagtcggcg ttcagaccgg tgttgtgctt
    24181 tatggatgcg ttacgctcct gatgacgtca tttgacgttc aacaacatca cggggccgca
    24241 cgacatttca cgtcagttag tgctatcgct caggaacaaa ttttcccgaa ttgggatatg
    24301 cccgcaaatt gctggtgatg tggggagaat ctggttgagt tcgggagaat tgatttggag
    24361 gcagaacgct taaattgtgg cgtccagaaa cgaaaaacgg acctccgtgg aggtccgttg
    24421 atatgaattt ggtgcggact cgggaatcga aaatttcctt tatcatattg atattaatgc
    24481 aatttgtttg gtttgattaa aaatgcaccc tcaattatac ccgcagtccc ttttgatatc
    24541 gaaagggaat cacactcggg atcaggctga gagcacagaa ggaaagtacc tggcttccct
    24601 ggcctgttca atacagggca gcagtgctgc cgaatccgtt ttatcctcat cgttgtcaaa
    24661 gataattccc gattcgcagt cgatattgtc ctgcagccac gcaatgagga tatacggaga
    24721 agcacatccg tatcatcagt gtgacgactg cggcggccat ccatccagtc atcggttcgt
    24781 ctgagaatga cgtacaactg cgcacgcgac acccggagac aacggctgac taagcttact
    24841 ccccatcccc gggcaataag ggcgcgtgcg ctatccactt ttttgcccgt ccatattcaa
    24901 cggcttcttt gaggagttca ttttccatcg ttttcttgcc gagcaggcgc tggagttctt
    24961 taatctgctt catggcggca gcaagttcag aggcaggaac aacctgttct ccggcggcga
    25021 cagcagtaag acttccttcc tggtattgct tacgccagag aaataactgg ctggctgcta
    25081 caccatgttg ccgggcaacg agggagaccg tcatccccgg ttcaaagctc tgctgaacaa
    25141 ttgcgatctt ttcctgtgtg gtacgccgtc tgcgtttctc aggccctaag acatcaatca
    25201 tctgttctcc aatgactagt ctaaaaacta gtattaagac tatcacttat ttaagtgata
    25261 ctggttgtct ggagattcag ggggccagtc tagttttttg ttatgttggc tgagtgacag
    25321 gatgttaatc tgatgtcttc tacttcatga agattatgtt ctaccagacg attgacgata
    25381 tcagttggtg ataaaaattc atctccacac ttaagaagag gtaggccagc agaaccatcc
    25441 cctggcagat aaattttatt gtatttttct ggtgttaaat cctttggtag gatagttttc
    25501 atctctgttg ccgcaatact tatagctgct gcggcagcaa catcggtgtt acttgattgt
    25561 agataacttc tgtttgcacc aggaaaggta aactctgttt tctttttatc aaaatcttgt
    25621 tttgtagctt cgtgcaggaa taatatatca taaggttttt tccctgttgt gactcttgaa
    25681 acattttgac caagaatatt aagagcatat tcacctacgg attttgttcc taaaaacaca
    25741 agcaatcctt ctttctctgg cactgtctct atttttctcc ctccaggaat tgtggagatg
    25801 gattgagatt gttctgccgg aattgatacg ctggcgctta taggtagctg agatcttata
    25861 ctaaacatac aacctccttc ttagtttctt taaatgtgag ttcatgataa ttctaatgga
    25921 gtgagtaata ttttagttga taatagctga tatccttttc agctattaat ctgtttattt
    25981 cagagtaaaa tttttattaa tattagattc gtattttgcg agtttcatga gattgccaga
    26041 tttggtcgct caatactaag gctagtgaca gacttattta ttagggacaa atttagcgag
    26101 gatatcaggt tcactagcga cataaatgct ttcatcagac agagttacaa tgccttcagg
    26161 ttggagcaaa tcactggtca gagaatgatt tccttttgtc aggtccatca tctcaacgaa
    26221 agacatttct gtcaggttaa acttgagtaa cttacgcgat tcatcggaca gaatcatcag
    26281 actttcatta ttaaagtcca ggccggatat atctttaaca tgtatatcat ttagttcctc
    26341 tggaatcgtt acttgttttg cactcagcag atctggagaa agctgatagg caaacatgcc
    26401 tgtagggcgt cgttctttag cgacaaacag catccggcct tctgctttcc atgccagtcc
    26461 ttcaaaccct ctattttttt tattgaattt ttctaactga attttatttc gcaaggagat
    26521 atgcatatct tttgtaactg taaaaaagaa tagttcagaa gtcttttcgt ctactgcagc
    26581 aaaaatgttc ccttgtatgt gctcaatggt ttcggcatca gagataaaag gcagcggggc
    26641 tcggccgatg atgtcgccac ttctggctat tttaagtagc tgcgcagggc tattctgggt
    26701 ggcatacagg aaatcatcct ctttattata cgtaagagat gataagttca ccgtatttgg
    26761 taattgtttt ataaatgaaa gtgagtattc ctgtggtgat tttttgtaac cataatcaaa
    26821 tacaaataat attaatgcaa ttggtgtgac aaacattgca ataaaaaatg caataaataa
    26881 gattcttttc attccttctc cattcgctat aaatggtttg cgaaataaat atctttgttt
    26941 tgtatgattg ctgtgaggtt aaattattaa taattgtgag tgtgattagt tattgtttgt
    27001 tttgataaat aaagtgtgtg gtgtccattt ttttattttt aaatgttttt caggtatgtt
    27061 tcagatgcgc cataccttgg ttgtagcgtt cttaccatct catcgatttc acatttcaca
    27121 ccttgtggca cttcatagga ggtcctgttc gtcaacattc ctaactcgtt ggggcggtct
    27181 tctggcgcca ttatcagaat cccggtatgg ctggcaacgt gaatgcttcc atctggtgat
    27241 tgagagatac ttatgttaat gcacgcgtta ggtacatcta acgatctttc cacattgtat
    27301 tctctggctt gagagatcct tgtatgtgtc aacattaagg ctggcacatt gaaagattga
    27361 tagaaagccg agcatatggc tgttgccgct tcggggtggc ataaaaactc cacaacttca
    27421 gctttctgtt tatcgctgcc gctgctttcc cttgctattt gctttgcatg atgacgagaa
    27481 ataccattat ttataacatt ccgggcaagt gatgaatgtg tgtctttaag tcctagtgca
    27541 tcgcgaagca atttttgtgc atctacaggt atatcaacac cattgagtga aagctttgca
    27601 ttgccgagtc ctcgaaatgt atccataaac atagagctga atggtgtagg cccggaaagc
    27661 ggatctgttt gagtattatg aggaatcaag gttgctgata gttttgacgt aaaactaagt
    27721 agattgtgca ttgacttatg atcagtcagg tccatcttag ataaggcacg atgaacctga
    27781 atgtatggca tgtggagatt gatgggtaaa gattgtaata acctattttg ttcgtttgac
    27841 tccaggagca taagaatctt ttcattatct ggtgtgcgaa tctcggcttc aacaagttcg
    27901 ctgttagtga cggaaattct taaggatgag ccatctgcaa aattagcata tattctgccc
    27961 aattcattag gcgcgggaga tacaggttct aaaacggtta acaactcttg aaatgtttgc
    28021 ctttcactac gagtatacgt tggattccat aaaccaaaac taagaacttt tagtaatttt
    28081 tcagcgcatg acatatcatt ccactgcttt ttaaaatcag aattaattct aattgcagca
    28141 tctaaaacta aagatgcgga attattatta agtccgttta tcatgagaga ttcccctgtc
    28201 tcatttatta tgaatgattc gacaacagat atcatatagc atgatatagt gtcaataata
    28261 tataagctat ttatagcgag agttttaaat gtatagagag ttattttttt aaattagagg
    28321 aaatactcct ctaatgacga acaatagatt ttcattgatt catcagcaat agtttttgtg
    28381 cctacgaaaa ggttgtaatt atttacatta ataatagcat tcagaaaaaa tttttattgt
    28441 gctgataaaa tataccatta gtgattagtt tagatacctg gctttcaggc aacttctttt
    28501 tgtctgtata taagggatga ttactaaggt ggtacattga tatcagttag ctttcccttt
    28561 tcgggttact ttatcaaaga gtcctcttta tagaagagcg cattgtgagt tttccagatt
    28621 gacttaaacg tgtggcggca tcacaccgat caacacgatc tgcagcaaag ggcaggtgta
    28681 accaggccca tcttagcgct atgcactttt gagccactga gcttgctaac cgtttacccg
    28741 gctttcactg agctaattac ggcgataatc tgatgcccag taaaacggac ttgatgtatg
    28801 acgggtcctc tttagcggga tagatatggg aatatttctt aatgcgaatg gtacggttat
    28861 gcggggggga caaacatggt tgcttgttct gccatttgtt ccttgttcct ttttataccg
    28921 ccgccgctcc cggtcagcac tggggaatag ggggttgagg tatatctgct ccttatgtca
    28981 ggggaacagg taatagctat atagcggaac gtcttagtgc tgtagttatt ccattt
//
LOCUS       DAZGOB010000053        28668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGOB010000053 DAZGOB010000000
VERSION     DAZGOB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.053"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            40..2076
                     /gene="yihQ"
                     /locus_tag="ACHLIF_002493"
     CDS             40..2076
                     /gene="yihQ"
                     /locus_tag="ACHLIF_002493"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO9606490.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDNDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            2122..3507
                     /locus_tag="ACHLIF_002494"
     CDS             2122..3507
                     /locus_tag="ACHLIF_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9606491.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            3550..4953
                     /locus_tag="ACHLIF_002495"
     CDS             3550..4953
                     /locus_tag="ACHLIF_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9606492.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            5021..5713
                     /gene="ompL"
                     /locus_tag="ACHLIF_002496"
     CDS             5021..5713
                     /gene="ompL"
                     /locus_tag="ACHLIF_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO9606493.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(5804..7069)
                     /locus_tag="ACHLIF_002497"
     CDS             complement(5804..7069)
                     /locus_tag="ACHLIF_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9606494.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(7171..8151)
                     /locus_tag="ACHLIF_002498"
     CDS             complement(7171..8151)
                     /locus_tag="ACHLIF_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO9606495.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(8159..8869)
                     /locus_tag="ACHLIF_002499"
     CDS             complement(8159..8869)
                     /locus_tag="ACHLIF_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9606496.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(9086..10909)
                     /gene="typA"
                     /locus_tag="ACHLIF_002500"
     CDS             complement(9086..10909)
                     /gene="typA"
                     /locus_tag="ACHLIF_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO9606497.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            11282..12691
                     /gene="glnA"
                     /locus_tag="ACHLIF_002501"
     CDS             11282..12691
                     /gene="glnA"
                     /locus_tag="ACHLIF_002501"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO9606498.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13010..14059
                     /gene="glnL"
                     /locus_tag="ACHLIF_002502"
     CDS             13010..14059
                     /gene="glnL"
                     /locus_tag="ACHLIF_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO9606499.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            14071..15480
                     /gene="glnG"
                     /locus_tag="ACHLIF_002503"
     CDS             14071..15480
                     /gene="glnG"
                     /locus_tag="ACHLIF_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO9606500.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            15592..15702
                     /locus_tag="ACHLIF_002504"
     CDS             15592..15702
                     /locus_tag="ACHLIF_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO9606501.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(15892..17265)
                     /gene="hemN"
                     /locus_tag="ACHLIF_002505"
     CDS             complement(15892..17265)
                     /gene="hemN"
                     /locus_tag="ACHLIF_002505"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO9606502.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKLW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(17454..17963)
                     /gene="yihI"
                     /locus_tag="ACHLIF_002506"
     CDS             complement(17454..17963)
                     /gene="yihI"
                     /locus_tag="ACHLIF_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO9606503.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(18082..18248)
                     /locus_tag="ACHLIF_002507"
                     /pseudo
     CDS             complement(18082..18248)
                     /locus_tag="ACHLIF_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18545..19177
                     /gene="yihA"
                     /locus_tag="ACHLIF_002508"
     CDS             18545..19177
                     /gene="yihA"
                     /locus_tag="ACHLIF_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO9606504.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(19559..22345)
                     /gene="polA"
                     /locus_tag="ACHLIF_002509"
     CDS             complement(19559..22345)
                     /gene="polA"
                     /locus_tag="ACHLIF_002509"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO9606505.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFSLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            22709..23641
                     /locus_tag="ACHLIF_002510"
     CDS             22709..23641
                     /locus_tag="ACHLIF_002510"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO9606506.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDQLLTSLMSSQRQDK"
     gene            complement(23682..25112)
                     /locus_tag="ACHLIF_002511"
     CDS             complement(23682..25112)
                     /locus_tag="ACHLIF_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO9606507.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(25267..25893)
                     /gene="dsbA"
                     /locus_tag="ACHLIF_002512"
     CDS             complement(25267..25893)
                     /gene="dsbA"
                     /locus_tag="ACHLIF_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO9606508.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(25910..26896)
                     /gene="srkA"
                     /locus_tag="ACHLIF_002513"
     CDS             complement(25910..26896)
                     /gene="srkA"
                     /locus_tag="ACHLIF_002513"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO9606509.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(26973..27242)
                     /locus_tag="ACHLIF_002514"
     CDS             complement(26973..27242)
                     /locus_tag="ACHLIF_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO9606510.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            27312..27896
                     /gene="mobA"
                     /locus_tag="ACHLIF_002515"
     CDS             27312..27896
                     /gene="mobA"
                     /locus_tag="ACHLIF_002515"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO9606511.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            27878..28405
                     /gene="mobB"
                     /locus_tag="ACHLIF_002516"
     CDS             27878..28405
                     /gene="mobB"
                     /locus_tag="ACHLIF_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO9606512.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         7033 a         7178 c         6931 g         7526 t
ORIGIN      
        1 aaggcaacaa tctcgttctc aactctatgg agattagtta tggatacgcc acgtccacag
       61 ttattagatt ttcaatttca tcagaataac gacagtttta ccctacattt tcaacaacgt
      121 cttattttaa cccatagcaa agataatcct tgtttatgga ttggctcagg tatagcggat
      181 atcgatatgt tccgcggtaa tttcagcatt aaagataaac tacaggagaa aattgcgctt
      241 accgacgcca tcgtcagcca gtcaccggat ggttggttaa ttcatttcag ccgtggttct
      301 gacattagcg ccacgctgaa tatctctgcc gacgatcagg gccgtttatt gctggaacta
      361 caaaacgaca accttaacca caaccgtatc tggctgcgcc ttgccgctca accagaggac
      421 catatctacg gctgcggcga acagttttcc tacttcgatc tgcgtggcaa accgttcccg
      481 ctatggacca gtgaacaagg cgttggtcgc aacaaacaaa cctatgtcac ctggcaggcc
      541 gactgcaaag aaaatgcggg cggcgactat tactggactt tcttcccaca gcctacgttt
      601 gtcagcacgc agaagtatta ctgccatgtt gataacagtt gctatatgaa cttcgacttt
      661 agtgccccgg aataccatga actggcgctg tgggaagaca aagcaacgct gcgttttgaa
      721 tgtgctgaca catacatttc cctgctggaa aaattaaccg ccctgctggg acgccagcca
      781 gaactgcccg actggattta tgacggagta acgctcggca ttcagggcgg gacggaagtg
      841 tgccagaaga aactggacac catgcgtaac gcgggcgtga aggtcaacgg catctgggcg
      901 caggactggt ccggtattcg tatgacctct tttggcaaac gcgtgatgtg gaactggaag
      961 tggaacagcg aaaactaccc gcaactggat tcacgcatta agcagtggaa tcaggagggc
     1021 gtgcagttcc tggcctatat caacccgtat gttgccagcg ataatgatct ctgcgaagaa
     1081 gcggcacaac acggctatct ggcaaaagat gcctctggcg gtgactatct ggtggagttt
     1141 ggcgagtttt acggcggcgt tgtcgatctc actaatccag aagcctacgc ctggttcaag
     1201 gaagtgatca aaaagaacat gattgaactc ggctgcggcg gctggatggc tgacttcggc
     1261 gagtatctgc ccaccgacac ttacctgcat aacggcatca gcgccgaaat tatgcataac
     1321 gcctggcccg cgctgtgggc gaaatgtaac tacgaagccc ttgaagaaac gggcaagctc
     1381 ggcgagatcc ttttctttat gcgcgccggt tctaccggta gccagaaata ctccaccatg
     1441 atgtgggcgg gcgaccagaa cgtcgactgg agtctcgacg atggcctggc gtcggttgtc
     1501 ccggcggcgc tgtcgctggc aatgaccgga catggcctgc accacagcga cattggcggt
     1561 tacaccaccc tgtttgagat gaagcgcagc aaagagctgc tgctgcgctg gtgcgatttc
     1621 agcgccttca cgccgatgat gcgcacccac gaaggtaacc gtcctggcga caactggcag
     1681 tttgacggcg acgcagaaac tatcgcccat tttgcccgta tgacctccgt cttcaccacc
     1741 ctgaaacctt acctgaaaga ggctgtcgcg ctgaatgcga agtccggcct gccggttatg
     1801 cgcccgctgt tcctgcatta cgaagacgat gcgcacactt acaccctgaa atatcagtac
     1861 ctgttaggtc gcgacattct ggtcgctccg gtgcatgaag aaggccgtag cgactggacg
     1921 ctctatctgc cggaggataa ctgggtccac gcctggacgg gtgaagcgtt ccggggcggg
     1981 gaagttaccg ttaatgcgcc catcggcaag ccgccggtct tttatcgcgc cgatagcgaa
     2041 tgggcggcac tgttcgcgtc gttaaaaagc atctaatcac atccgcccgt ggggaaactc
     2101 atgggcaaag ggagaacatc gatgagtcac atcacaacgg aagatccagc aactttacgc
     2161 ctgcccttta aagagaaact ctcttacggt attggcgacc tggcctctaa catcctgctg
     2221 gatatcggta cgctttatct tttgaagttt tataccgacg ttctggggct gccaggcacc
     2281 tatggcggca ttatcttttt gatttcaaaa ttctttactg cgtttaccga tatgggtacc
     2341 ggcattatgt tggattcccg acgcaagatc ggtccaaaag gtaagttccg tccttttatt
     2401 ctgtatgcgt cattcccggt caccttactg gcgatcgcca actttgtcgg cacaccgttt
     2461 gatgtcaccg gtaaaacggt gatggccact attctgttta tgctttacgg actgtttttc
     2521 agcatgatga actgctccta cggcgcaatg gttcccgcta tcaccaaaaa ccccaacgaa
     2581 cgcgcctcac tggcggcatg gcgtcagggc ggcgctacgc tgggcctgct gctgtgcacg
     2641 gtgggattcg tgccagtgat gaatcttatc gagggtaatc agcaacttgg ctatatcttc
     2701 gccgccacgc tgttttcact gttcggcctg ctgtttatgt ggatctgcta ctcaggcgtg
     2761 aaagagcgtt atgtcgaaac ccaaccaacc aatccggcgc aaaagcctgg cctgttgcaa
     2821 tctttccgcg caattgccgg taaccgccca ctgttcattc tgtgtattgc caacctctgc
     2881 accttagggg cgtttaacgt caagctcgcc attcaggtct attacaccca gtacgtactt
     2941 aacgatccca tcctgttgtc gtatatggga tttttcagca tgggctgtat tttcatcggc
     3001 gtgttcctga tgcctggcgc agtcagacgt tttggtaaga agaaggtcta tatcggcggc
     3061 ctgctgattt gggtgctggg cgatctgctc aactatttct tcggcggcgg ctcggtcagc
     3121 tttgtggcgt tctcctgcct ggcgttcttc ggctcagcgt ttgttaacag cctgaactgg
     3181 gcgctggttt ccgacaccgt cgagtacggc gagtggcgca ccggcgtgcg ttcggaaggc
     3241 acggtctaca ccggcttcac cttctttcgc aaagtgtctc aggcgctggc tggtttcttc
     3301 cccggctgga tgctgacgca aattggctat gtgccgaacg tcgcacaggc tgaccacacc
     3361 attgaagggt tacgccagtt gatcttcatc tacccaagcg cactggcggt agtcaccatt
     3421 gtggcgatgg gttgcttcta cagcctgaac gagaagatgt atgtccgcat tgtggaagag
     3481 atagaagccc gtaaacgcac ggcgtaataa taataaacga cgccctgcgg ggcgttataa
     3541 ggagtgatta tgtctgacca taatccactg acattaaaac tgaatctgcg ggaaaaaatc
     3601 gcctatggta tgggcgacgt cggttcgaat ttaatgctct gcatcggtac tctgtatctc
     3661 ctcaaatttt ataccgatga attagggatg cctgcttact atggcgggat tatctttctg
     3721 gtggcgaagt tttttaccgc gtttaccgat atgctcaccg gatttttact cgactcgcgg
     3781 aaaaatattg ggcctaaagg caaatttcgt ccctttattt tatatgcagc agttccggcg
     3841 gcgttaattg caacgctcca gtttatcgcc accacctttt gtttgccggt taaaacgacg
     3901 attgccaccg cgctgtttat gatgtttgga ctttcataca gtctgatgaa ctgctcgtat
     3961 ggtgcgatga tcccggcaat taccaaaaac ccgaatgagc gcgcgcagct cgcggcttac
     4021 cgccagggtg gtgcaaccat agggctattg atttgtaccg tggcgtttat tccgttgcag
     4081 tcgctttttt ctgactcaac cgtcggttat gcctgtgcgg cacttatgtt ctccattggc
     4141 ggctttattt ttatgatgct gtgctacaga ggcgtcaaag agcattatgt ggacacagcg
     4201 ccaaccggac ataaagccag tattctcaaa tctttttgcg cgatatttcg taatccacca
     4261 ttgctggttt tatgcattgc taacctgtgt actctggcgg catttaatat caaactggcg
     4321 attcaggtct attacaccca gtatgtgctg aatgatatta atttattgtc gtggatggga
     4381 ttcttcagta tgggatgcat cctcgtcggc gtattactgg tgccagtaac tgtaaaatgt
     4441 tttggtaaaa aacaggttta tttagctggc atggtgctat gggcggtggg tgatatactg
     4501 aattatttct ggggaagtaa ctctttcact ttcgtcatgt tctcttgtgt cgcctttttt
     4561 ggcacggcct ttgtaaacag cctgaactgg gcactggtac cagataccgt agattacggt
     4621 gaatggaaaa ccggtattcg tgccgaaggt tctgtttata ccggttatac cttctttcgt
     4681 aaaatttctg ccgcacttgc tggcttcttg ccaggcatta tgctgacgca aattggttat
     4741 gttcccaaca tcgcgcaaag cgatgcgaca ttacagggtt tgcgtcagct catttttatc
     4801 tggccttgtg cactggcaat tattgctgca ttgacgatgg gattctttta cacactcaat
     4861 gaaaaacggt ttgcattgat tattgaggaa atcaaccaac gcaaaaataa agagatggcg
     4921 acagaagaaa aaacggcttc cgtaacgtta taaatgtcaa tagccgctat ttccatcctg
     4981 gtgggtggcg gcctccctac gtttaaaaaa tggacttatt atgaaaaaga ttaatgcaat
     5041 aattttatta tcttctctga cctctgcctc ggtatttgct ggcgcatacg tcgaaaacag
     5101 ggaggcatat aatcttgcct ccgatcaggg cgaggttatg ctacgtgtgg gttataactt
     5161 cgatatgggc gcgggtatta tgttaaccaa tacctacacc tttcagcgag aagatgaact
     5221 aaaacatggc tataatgaaa ttgaaggttg gtatccttta ttcaaaccaa ccgataaatt
     5281 aaccatccag cccggtggct taattaatga taagagtatc ggttcgggtg gtgcagtgta
     5341 tctggacgtc aactataaat ttgtaccatg gtttaatctg acagtacgaa atcgctataa
     5401 ccataataac tatagttcaa cagatttgag cggggaactg gataataatg acacctatga
     5461 aattggcacc tactggaatt ttaaaatcac cgataaattt tcctatacat ttgagccaca
     5521 ttatttcata cgcgtaaatg actttaatag cagcaatgga aaagatcatc actgggaaat
     5581 cactaatacc ttccgttatc gcattaatga acactggctg ccttatttcg aattacgttg
     5641 gttagatcgt aatgtcgaac cgtaccaccg cgagcaaaac cagattcgta tcgggacgaa
     5701 gtatttcttc tgatgtgaaa ctgccggatg cggcgtaaac gccttatccg gcctacggtt
     5761 gatacggttt tcgttggccg gattaagcac atccggccat taatcaagct tcagccgcca
     5821 gcatctcttt cttacgttgg ctaattcgct taaccagcat tacggaaacc accgcaccac
     5881 agaatgcgaa gcaggccata atgccaaaca cgattttgta gccaataatc cccggattta
     5941 aatccagaat gtagccatac agactgaagc agaacatcgc cggagcgtaa ccaataaagc
     6001 tacccaacgc catcgccgcg cctgttttat tttcagcaat ttttgcttcg ccgataggtg
     6061 caaaaaatac ggctcgctgt gtaaagacta tcgcgccaaa gcccagcgta catgccatcc
     6121 ctaaatagac cggcatactt tcgtgcggca gcataatcaa cagtacgagc gcagcggtac
     6181 tgatgataaa ggtgtagcat agatatttac tcggcgattt caggatctta tctgaaatca
     6241 tgccaccaat cggtccacca atcattttca gacagtattg gttaatgatg ccgtaagccc
     6301 ccaccagcgc aacgggcaat agatagatgt ttttcaggaa tggaatgaag aatgtcaggc
     6361 cacagtaaac cgcgtaaacg aagaagacgt taaaagcgat aagccagatg gttttgtctc
     6421 tcagcaccga ggtcatactg gtgttttgcg atgctccgtc ttcttttttc acctcaacgg
     6481 acggtgcctc ttctttgtca ttcaggacaa agaaaataat aatgcctacg gcaatcacaa
     6541 taagggaata gaaccagatg cctgctttaa aacctaataa gccactgcca aaccaggtaa
     6601 atactgccaa cgcagaaaat gccaccacgg tatcgacaat gccacgccct gtttcgaaga
     6661 agccaaacaa ccgaccttgt tgttcgctat tgcccaatcg acttaccgac ttgagcaaga
     6721 ccggccagtt catcatgtcg caagtaacgc caaatagcgc ccagacaaag aggatgcccc
     6781 aataccccgg cattgtcgtc aaataaacac ccagtaatcc tgtcgcaatg agtgagaatg
     6841 acatggtgta tctgcgcggt agtttatcgg caaaataaat agacagaaaa aagcccactg
     6901 tggtgacaaa tgagtttacc gacatagcat taccaatttg accattggtc aaatggaaat
     6961 attcctgcat cgggatataa aacgcatctt tcagcgacgg taatttataa attgtaccgc
     7021 cacacaatgt taatagacta aataacgccc atttcttttt cgtgagcata acatccccta
     7081 agcgattttt aaaatcactg ttttaaaggc aataagatac cgaccattca tatggaatgg
     7141 ttttattaac tgcgtatgag ttaaaactta ttagtggttc agaagatggt ttgcctcttg
     7201 tttgatttgt tgtagtacgt tacgcacatg atttatctga ttaattgcat cttctttttc
     7261 tttacgtaac cgcgcatcca gtaaatgatt ttctggcaat ggtgtttcac tcatctggcg
     7321 ataagggatc cacggattat catcttcgtc ttcaaagcgg aacgccggcg catagtaatc
     7381 aacctcttct tccaggccat atgccgtcac ctgcggctca tcatcaccca ggcatataag
     7441 gtgcgttaat aacgctttga acggcatatc accctgacct gaaatacagg ctttatgacc
     7501 caggccaccc ggttctttaa cgatcaaggc atctttgata tggacctggg taatatgcgg
     7561 cgccatcgtt tttaaagcgt cgatgggatg ctcatttgca ttgatcatgt tcgcaaaatc
     7621 aaacagtaag gaaagagatt ccatctcact ttctttgacc agcgacacca gctcatggct
     7681 ttttaaatct tcatgctgtt cgatcgtaaa agtcaggccg ctgtcctgat acgtttcccg
     7741 tacataggca atgtcgttag cgatgatcga taatacgtcg cgcaaattac cttcataacg
     7801 tggataaaaa cgtacggacg atgccccagt tttcaacgca atggcgacgg cttcgtcaat
     7861 agatgcctta tctgaagcgc tggtttcaat atgaatatca aggttcagac ggcgggcttt
     7921 atccccaaag gcagagagtt ctttatcgtc catattacca agagaaaaac gctcgccatc
     7981 cagaacatgg attttgacgc cacgtagatt attttcactt gcgatatcaa gtaaatcagc
     8041 aggcaacact ctttccagcc gcatatttaa atgaaaggca taagcatgta aataaagagg
     8101 aagagtgtcg acacgttgta gaatctttct tgcgttattt attgtcacca taaaacacct
     8161 acagattaat gatgaggtct acggcctcaa atcgccaata ttcaatatct atctggacaa
     8221 tattgccttg ctggtcggcg cgatgctttt caattaagat cgctggcata cccgcagaac
     8281 cgccaagata cttgctgata tcgcccggca ttctgacggg tttgaaaatc aattttttta
     8341 ctaccgtctc tttttggtag cacttttccc agactgcaga aaatgagtgg ttttcaagtt
     8401 gttcaataaa acccggagca acttcaggat taatatatgt ttcgtgatag aaaactttat
     8461 gtccttccag cgccccccag ccggtgatgc gatagagttc agttgatggt gtcactgcaa
     8521 tcagtggcgc gagcgtcttg ggaatatcgc tggtacggtt tttctcggta aacccccagg
     8581 aaggctctcg tccttgctca attgcggccc gctgaaagct cgccgacaac tccggactgt
     8641 aattaaatcg cggctgggtc acgaaccacc ccttacgatc cttacggaag attttggatt
     8701 ctgcctcgag gttcaacaac gcctggcgca gcgtcatacg tttaatgccc agcagttctc
     8761 ccagctctct ttctgaaggg agttttccac ccggcgtggt gatgccatct ttcaaccacc
     8821 gatccagttt ctcttttgca ttttctacgg tggattgatt ctccgccatt gtttaatcct
     8881 tttggtttaa accagatgaa gcatcttacg cttacgccaa aaaagaaagc aattaacggc
     8941 gtatgacttt gcagataatt gtgacaaagc tctctattga cgtaatccca gacttataag
     9001 ggagatactc gcattggtgt gcgctcacga caggtaaaaa aaaacctgcc agcgatggca
     9061 ggttttttat aactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg gcgcggcgac
     9121 gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc acttctacca
     9181 gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata ggcggaacca
     9241 gaacaacggc ttcgtcagta ccggaagcac gcatgttggt cagtttctta ccggtcaggc
     9301 agtttaccgt caggtcgtta gagcggctat gaataccgat aatctgacct tcgtaaactt
     9361 ctgcaccgtg accgaggaac agcttaccgc gatcctgcag accgaacagc gcgaacgcga
     9421 ccgctttacc ctgaccgtta gagatcagta cgccgttctg acgctgaccc acttcacccg
     9481 gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg gaagtcatgg
     9541 tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag tcgagacgta
     9601 cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg cccagcgcct
     9661 gcattacaga accctgatgc tgttcttcaa cgtccagagt cacgttttca tacggctctt
     9721 gtttacgacc gtcgatttca cggaagataa ctttcggacg ggataccgcc agttcgaaac
     9781 cttcacgacg catgttttcg atcagaacag acaggtgcag ttcgccacga ccagaaacgc
     9841 ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt accagttctt
     9901 tgttcagacg atccaggatc tgacgagacg ttacgaactt accttcttta ccgcagaacg
     9961 gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag agtgccggca
    10021 gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg ccaaggcccg
    10081 tgatcgcaac gatatcgcca gcttccgcca gatcggtttc gatacgttcc agaccgaggt
    10141 ggcccagcac tttaccgact ttcgcgttgc gggttttgcc ttcgctatcg atgatagtga
    10201 cctgctggtt cggcttcact ttaccgcgct tgatgcggcc aatgccgata acgccaacat
    10261 agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg tcaacgtccg
    10321 gcgcaggaac gtggtcaaca atcgcctggt acagcggggt catgtcttcc gccatatctt
    10381 cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg aagtccagct
    10441 gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca acccaatcag
    10501 gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg ccgtaagcaa
    10561 acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg tcaaccacca
    10621 gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag tcggcgtgcc
    10681 ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg tttttcgcga
    10741 ggatggtaat cccccgctct ttctccaaat cgttggagtc catcacgcgc tcttgggttt
    10801 cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccagg gtggttttac
    10861 catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca actttgcctc
    10921 aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa caggatcaca
    10981 aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac ggagcataaa
    11041 aagggttatc caaaggtcat tgcaccaaca tggtgcttaa tgtttccatt gaagcactat
    11101 attggtgcaa cattcacatc gtggtgcagc ccttttgcac gatggtgcgc atgataacgc
    11161 cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta cggcgacacg
    11221 gccaaaataa ttgcagattt cgttaccacg acgaccatga ccaatccagg agagttaaag
    11281 tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt ttgttgattt
    11341 gcgcttcacc gatactaaag gtaaagaaca gcacgtcact atccctgctc atcaggtgaa
    11401 tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg gctggaaagg
    11461 cattaacgag tccgacatgg tgctgatgcc agacgcatcc accgcagtga ttgacccgtt
    11521 cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca ccctgcaagg
    11581 ctatgaccgt gacccgcgct ccattgcgaa gcgcgccgaa gattacctgc gttccactgg
    11641 cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg atgacatccg
    11701 tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag gcgcatggaa
    11761 ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga aaggcggtta
    11821 cttcccggtt ccaccggtag actcggctca ggatattcgt tctgaaatgt gtctggtgat
    11881 ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg ctggtcagaa
    11941 cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc agatctacaa
    12001 atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc gcgaccttta tgccaaaacc
    12061 gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta aaaacggcgt
    12121 taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt actacattgg
    12181 cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca ccaactctta
    12241 taagcgtctg gtcccgggct atgaagcacc ggtaatgctg gcttactctg cgcgtaaccg
    12301 ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta tcgaagtacg
    12361 tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga tggccggtct
    12421 tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc tgtatgacct
    12481 gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag aagcactgaa
    12541 cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcactg acgaagcaat
    12601 tgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc gtgcgtatga ctccgcatcc
    12661 ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc cggcgcaaca
    12721 acgccggagg ttcaaaccag gcccatctgg cgtaattgtt gcagccagtt tgaacacgga
    12781 cagcgcgcag aacccggagc gtacactggt acgtgaggag ttcgagcact gcccaggttc
    12841 aaaatggcaa ataaaatagc ctgatgggac tggtttttag ttgccgtgga aatttcagcc
    12901 catctctgca tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta
    12961 aaaagctata atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg
    13021 cacgcagccc gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaatcga
    13081 tgacaacctg gcgatccatt acgccaaccc tgccgcgcaa caactgctcg cccaaagctc
    13141 ccgcaaattg tttggtacac cgttaccgga actgttgagc tacttctcat taaatatcga
    13201 gctgatgcaa gaaagtctgg aggcggggca aggttttacc gataacgaag tgacgctggt
    13261 catcgacggg cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat
    13321 cctgctggag atggctccga tggataacca gcgccgctta agtcaggaac agctacagca
    13381 cgcccagcag gttgctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc
    13441 gcttggcggt ttacgtggcg cggcgcagct gctcagcaaa gcgttacctg acccatcact
    13501 actcgaatat accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatc tggtcgaccg
    13561 tctgttgggg ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga
    13621 acgcgtggta acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta
    13681 cgatcccagc ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa
    13741 tattgtgcgc aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac
    13801 ccgcaccgcg tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga
    13861 tgtggaagat aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat
    13921 ggtcagcggc cgcgaaggtg gcaccgggct tggcttatcc atcgctcgta atttgattga
    13981 tcagcattca ggcaaaattg aatttaccag ttggccaggg cataccgagt tctcggttta
    14041 cctgcctatc aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat
    14101 gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg ctggggcagg tttaacctgt
    14161 acgacgtttg agaacggcgc agaagtgctg gaggcgctgg cgagcaaaac gccggatgtg
    14221 ctgctttcag atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa
    14281 cagcgccatc caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc
    14341 gtcagcgcct atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa
    14401 gcagtggcgc tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat
    14461 gttcagctta acggcccaac gaccgatatc atcggcgaag cgccagccat gcaggacgtg
    14521 ttccgtatta tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc
    14581 ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg cgccaaagcg
    14641 ccgtttatcg cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt
    14701 ggccacgaga aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag
    14761 gccgatggcg gtacattatt cctcgacgaa attggtgata tgccgctgga tgtgcagacg
    14821 cgtttgctgc gcgtgctggc agacggtcag ttttaccgcg ttggcggcta tgcgccggtg
    14881 aaagtggatg tgcggattat cgctgccact caccagaatc tcgaacagcg agtgcaggaa
    14941 ggtaagttcc gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg
    15001 ctgcgcgaac gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg
    15061 cgcgaactgg gcgtagaagc gaagttactg catccggaaa ccgaagctgc tctgacgcgt
    15121 ctggcgtggc caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg
    15181 gccgccgggc aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt
    15241 gcggagagta cttcgcaaat gcaaccggac agctgggcga cgcttcttgc gcagtgggca
    15301 gacagagcgc tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag
    15361 cggacgttac tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg
    15421 ctactcggct ggggccgcaa caccctgacg cgtaagttaa aagagctggg gatggagtga
    15481 ttcacagctt gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttacgcttta
    15541 ctgttccgat aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa
    15601 tcaataatta atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt
    15661 gctgccgtgc tgtgtgccgc aatgattggg ctgtttagtt gagtttcaga ttgctgatga
    15721 agtgctcttt gttcatgccg gatgcggcgt gaacgcctta tccggcctac aaaagcaata
    15781 ttttcaacag gttttgtagg cctgataagc gtagcgcatc aggcaatctt gttctcagca
    15841 cattgatggc gctttcgttt tctactttgt aaacgaagcg ccattcacta tttaaatcac
    15901 ccgtgagaac tgctgcatcc gcgctttctg gcgcagatag gtatcaaagc acatgcaaat
    15961 gttgcggatc agcaagcgac ctttcgccgt cacctgtatt cccttctcat ccacatccac
    16021 cagcccatct tttgctaacg gggcgagcag cttgagatct tccgcaaagt aatcagcgaa
    16081 gtgcaaatcc cacagtttct caataggggc gtaatccaga cggaagttgc agatgagcga
    16141 cttaatcaca tcgcggcgaa tacagtcatc acgcgttagc gcaataccac gccacagcgc
    16201 attgccttgt tcatccactt gctgatagta ctgcttcaac tctttctggt tctgcgcgta
    16261 gcagtcgcca atcatgctga tggcggaaac gcccatcccc agcagatcgg tatcgccctg
    16321 agtggtgtag ccctggaagt tacgatgcag cacgccttca cgctgggcca ccgccagctc
    16381 gtcatccgga cgggcaaagt gatccatacc gataaactga tagcccgatt gcgtcaggaa
    16441 ggcgatggtt tcctgcagga tatcgagttt ttgctgcgga ctcggcaggt cagcatcttt
    16501 gattttgcgc tgagcagcaa aaatggtcgg cagatgcgcg tagttaaaga cactcagacg
    16561 atcggggttc agctccgcca cacgtttcag ggtaaaggcg aaactctccg gcgtctgttt
    16621 cggcaggccg taaatcaggt cgatgttggt ggaggtaaaa ccaatctcac gcgcatggtt
    16681 aagcagtgca aagatgaact cttcatcctg ctcgcggtta accagacgtt gcacttcttt
    16741 gttgaagtcc tgcacgccca tgctcaggcg attaaagcct tcggcgcgta aatgatcgag
    16801 tacatccagt tcgatttccc gcggatcgac ttcgatcgaa atctccgcat cggcattgaa
    16861 ctggaagttt tcgcgcagca gcttcatcag gcggctgatt tgcgctttat tcagatacgt
    16921 cggcgttccg ccgccccagt gcaattggct gacgtgacgc ccggcgaaca gcggtgcacg
    16981 atggacgatt tcttgctcca gcgcgtccag atactgatcg gccttgtgct gctggcgagt
    17041 aacaatctta ttgcaaccgc agaagtaaca aagcttatgg cagaacggga tatgtacgta
    17101 gagagataat ggacgctcag gatagcgcgc cacggcttgt aaaaacgcct gttcgccgaa
    17161 gtcttctgaa aactccagcg cggtcgggta cgaggtgtat cgtggcccgg aatagttata
    17221 tttctggatc agggccagat cccagtcgat ttgctgtaca gacatgctca ctccttccga
    17281 tggcgtctct ggcggctacg gcgtaccggc ggatgcccgt ttcgggtcat cagccggttg
    17341 cgcagccact tttgtcgccg cgacaaccgc tgtagtttaa cgaataacca caccagataa
    17401 catataaccg gaagggttat cagcagaacg ggaaggccca cggcggcaat ccgttagttg
    17461 ccccgcagca gacgcatcat gtcttcttgc ttctcgtctt cttcctcttc ttcgtcatcg
    17521 tcataagaga ggccgagttt ctgcatcaac tcatcaatac gatccagttt agcatccacc
    17581 caggattgct cttcggcgct cagggtttcg cctgcttcca gacgttccag cagcgcatcc
    17641 agacgctcat ccgtttccag taattccaac tccgcctgcg gtgaaagcat aggtttctca
    17701 ctcttcggtt tgtgctgttt ggtgactttt tcagtcacgc ccaatggaat aggggtttta
    17761 ctgccaatac gtggatcttt tggtgcgttc tggcctttgc tgcctgacgt ggtgtttccg
    17821 cccgctgcgc ggctgcccgg cgcatgacca cgacgttttt tctgacgctt gcgatcacga
    17881 gcttcctgat ccagctcctc gcgtgttttt cgacgcgctt ttgcatgacc tttgctgcgt
    17941 gagtttgaag atgatggttt cataatggtt cgtcttttag ccgttttatt cagtatagat
    18001 ttgcggcgga atctaacaga aagcaagcaa agaaaaaagg cgacagatta ctctgtcgcc
    18061 ttttttcctg actcataacc cttaacgggt cttacaatcc ttgcaggccg ccaacacacc
    18121 ctgtttttcc tttagccgga acaatccgtg ttgattccat ttccgtttaa ttacgtctcc
    18181 ggacgtttgt cttcctgaca atcctctgtc ttcgcctcct ggcgctcctg accctcatcc
    18241 tgagtcattg ttcctgttag cgtcctcgct ctatgggtgc tactttacgc ctttgcttta
    18301 aacaaacaat caaccgccat cagtatttgc acattttcag agttttatat aaaagcaatt
    18361 cactgattta tatagcttat ttgacatgca tcgcagaatt ttctctgcta tttcgcataa
    18421 agactatcgg caatgtctca caaagcgtat ggcttttact tacaacgctc acgccgaaaa
    18481 atccgccttt tattatggtt caggtataat cgccacaaag ctttgactga cggagacgac
    18541 cgctttgact aatttgaatt atcaacagac gcattttgtg atgagtgcgc ctgatattcg
    18601 ccacctacct tccgataccg gaattgaagt ggcttttgca ggccgttcca acgcaggtaa
    18661 atccagcgcg ctgaacacgc tgactaacca gaaaagcctg gctcgtacct caaaaacccc
    18721 agggcgcacc cagcttatca acctgtttga agtggctgac ggcaagcgtc tggttgactt
    18781 gcctgggtac ggttatgcgg aagtcccgga agagatgaag cgcaaatggc agcgtgcgct
    18841 cggcgaatac ctcgaaaaac gtcagagcct gcaaggtctg gtggtgctaa tggatattcg
    18901 ccatccgctg aaagatttgg atcagcagat gattgagtgg gcggtagaca gcaatatcgc
    18961 cgttctggtg ctgctgacca aagcggacaa actggcaagc ggcgcacgta aagcgcaatt
    19021 gaatatggtg cgtgaagctg tactggcgtt taacggtgat gtgcaggttg aaacgttttc
    19081 ttcgttgaag aaacaaggcg tggacaagct gcggcagaaa ctggatacct ggtttagcga
    19141 gatgcagcct gtagaagaaa cgcaggacgg cgaataattt tcttgcctta atgcttgtgc
    19201 cggatgtggc gtatccggcc cgtaaattca atagattaca attctaccgt tggcctgata
    19261 aacatagcgc atcaggcatt acggatcttt tctttcgccc aataaaaaac gccccagtca
    19321 ttactgactg gggcggctaa aatattcagc caaatccgat tacgtgaagt aaaaggtctg
    19381 aaagatagaa catcttacct ctgtacccta cgcgactaac tttactcttt tttgttcagt
    19441 tcagaaagca ctttttgtaa ttttttttca gttgttgcat aggaatttct aatagccatc
    19501 acaaaacgtg acagcttatg ttgcttactt acgaaaaaag ggcatgttca ggcgaatctt
    19561 agtgcgcctg atcccagttt tcgccactcc ccacttccac cagcaacggc acatccagac
    19621 gggtacagtt ttccatcagt tgatgaatct gcttcgcgac ggcatcaaca tcatctttat
    19681 gaacttcaaa taccagttca tcgtgtacct gcatgatcat acgtacacgc ggttgctcag
    19741 cctgtaacca cgcatcaacg gcaatcatcg cccgtttgat aatgtcggcg gcggttccct
    19801 gcattggcgc gttaatggct gcacgttcag ccgctgcacg acgagcacca ttgctggatt
    19861 tgatatccgg cagatacaga cggcgtccgt ccagcgtttc aacgtagccc tgctctttcg
    19921 cctgagcacg ggtgcgttcc atatactcca gcacgccagg gtagcgttcg aagtaaaggt
    19981 ccatgtactt ctgcgcttct ttacgtggaa tgttcaattg ccgcgccaga ccgaaagcac
    20041 tcatgccata aatcagacca aagttgatcg ctttcgcgct acggcgttgc tcgctggtga
    20101 cggtttccag tggcaaacta aacacttctg ccgccgttgc ccggtggata tcttttcctt
    20161 ccgcgaatgc ggtcagcaag cctttgtcac gcgaaagatg cgccataatg cgcagttcaa
    20221 tctgcgagta gtccgctgag acaatcacat aatcctctgg cgcaataaac gcctggcgga
    20281 tacgacgacc ttcttcgtta cgcaccggaa tgttttgcag gttaggatcg gttgacgata
    20341 aacgtcccgt tgcagttact gcctggtgat aagaggtatg cacacgcccg gttttcgggt
    20401 tgatcatcag cggcagcttg tcggtgtagg tcgatttcag cttcgccaga ccacgatact
    20461 ccagaatcac ttttggcaac ggatagtcca gcgccagttc ttccagtacc tcttccgacg
    20521 ttgacggcgc gccacccggc gttttcttca gcggtttaat accctgtttt tcaaagagaa
    20581 tggtttgcaa ctgcttggtg gaagaaaggt taaattcttc acctgcaatt tcatgcgctt
    20641 tcttttccag ctcagccaga cgcaaggtga gctcttcaga atgattgtgc agcactttcg
    20701 gatcgatctt cacaccgtta cgttcaatgc gtgaaagcac cggcaccagc ggcatttcga
    20761 tattctcgaa gacgttcaac ggccctttgt gtttttgcag atctggccac attttcagat
    20821 gcaactgcaa ggtgacatca gcatcttcgg cggcgtaacg tccggcctct tcgagggcaa
    20881 tctggttaaa ggtcagttga tttttgcctt tacccgcaat ctcttcaaaa gtgatggttt
    20941 tgtgcttcaa ccaacgttcc gcgaggctgt ccatatcgtg acgcccggca acgctattga
    21001 gaatgtagga ctccagcatg gtatcaaacg caatcccacg cagctcaatg ccgtaattcg
    21061 ccagaatacc gcgatcgtat ttcaggtttt gcccgacctt cagcgccttt tcatcttcca
    21121 gcagcggttt tagcaactcg agtgcacgct cgcgagagat ttgatcgggc gcgtcaagat
    21181 aatcatgagc aaccggaata tatgccgcta cgcctggctc gatagcaaaa gaaagcccga
    21241 ccaggttagc agagatgtta tcaaggctgt cggtttcggt atcaaatgca aataccggcg
    21301 ctttttccag cttcgcaatc cacgctttca gtgtttcttc atcaaggatg gtgacgtagt
    21361 tgtcataaga aatcaccgtt gccgtcactt ctggcgcttc gtctgcaaca ctggtttcct
    21421 gcggcttcgc ggctggtttt gcccctttgg cctgtaacca tttgcccgct tcgacatcag
    21481 cagtccagcg tttgaactca tactttttga acagccccaa caactcttcc gctgccggtt
    21541 gctgcacttc cagttgttca caggtcagct ccagttcaac gtcggtttta atcgtcgcca
    21601 gctggtatga gagataagca acttctttgt tttgctcgag cttcgctgcc attgttttcg
    21661 cgccacggaa gctcaaccca gcaatttttt ctggctcggc atacagcgta tccagcccgc
    21721 caagaccttg cagcaatgcc tgcgcggttt tttcaccgac gcccggtacg ccaggaatgt
    21781 tatcggagga gtcacccatc agcgccagga aatcgatgat cagttctggc ggcacgccgt
    21841 acttattcac cacctcttcc ggtccgagga tggtattcgt catggtattg ataagcgtaa
    21901 tatttggcgt caccagctgc gccatatctt tatcgccagt gctgatcagc accggacgcc
    21961 cggctttttc ggcttcgcgc gccagagtac cgataacgtc gtccgcttct acgccagaaa
    22021 ccgccagcag cggcagtccc atcgctttaa ccatcgcgtg caggggttcg atttgtgctc
    22081 gcagatcgtt cggcattggc gggcgatgtg atttgtaatg ttcaaacagt tcatcacgaa
    22141 aggtttttcc cttggcgtca aagaccaccg ctgcatgcgt cggtttatat tgcatgatca
    22201 gactgcgcag catgttgagg acaccataca tcgcaccggt cggctcgcct gcgctgttag
    22261 tcagcggggg aaacgcgtga tatgcgcgat aaagataaga tgaaccatct acaaggataa
    22321 gtggattttg ggggatctga accataatgt ccgtgcctgt ttatcagatt atgggtaaag
    22381 gatgccacag acaggatgaa aacatgagtt tttcaccgct tttcgtgaaa atcttttgta
    22441 gatcttctgg atcgctcgca aatcgtcatg tggataactt tgtgcataaa ttatttacat
    22501 cgcttttaat ttattatcat gctttttaca gcatgatatt tgtctttgtt tttcatgcag
    22561 ttaaaaataa agccatccct aataattgag ataatgggat tttttctaat gtggatagat
    22621 atgaattatt tttctcctta aggatcatcc gttatttggg tcgttttctg ctaaaatctg
    22681 cgccttgcgc ttctttagcg cactgtctat ggctaacctt ttgaataaat tcattatgac
    22741 gagaatactc gctgcgataa cccttttgct gagtatcgta ttgaccattt tggtcactat
    22801 tttttgttcc gtcccgatca tcattgccgg gattgtaaaa ctattgctac ctgtgccagt
    22861 catttggcga aaggtttcgc gtttttgtga ttttatgatg tattgctggt gtgaaggtct
    22921 ggcggtatta ctgcatctga acccacacct gcaatgggaa gttcacgggc tggaagggtt
    22981 aagtaagaag aactggtatc tgcttatttg taaccaccgt agttgggcag atattgtcgt
    23041 actgtgcgtg ctgtttcgta agcacattcc gatgaataaa tatttcctca agcaacagct
    23101 ggcctgggtg ccatttcttg gcctggcgtg ctgggcgctg gatatgccgt ttatgaagcg
    23161 ttattcccgc gcttatttat tacgtcatcc ggagcgacgt ggtaaagatg ttgaaaccac
    23221 tcggcgttct tgtgaaaagt ttcgcctgca tcccaccacc attgtcaatt tcgttgaagg
    23281 ctcccgtttc acgcaagaaa aacatcagca aacccactcc acttttcaaa acttgttgcc
    23341 accaaaggcg gcaggcattg cgatggcgct aaatgtactg ggtaaacaat tcgataaact
    23401 gcttaatgtt acgctgtgtt atccggacaa taaccgtcag ccgttcttcg atatgttaag
    23461 cggtaaactg acgcgaattg tcgttcatgt ggatttacag cctattgccg atgagttaca
    23521 tggcgactac atcaatgaca aaagctttaa gcgccatttt cagcaatggc tgaactcact
    23581 gtggcaggaa aaagaccaac ttctgacgtc gttgatgtca tcacagcggc aggacaagta
    23641 aattttgctc gcctctgaat gagcagaggc gagtgagtat tttaatgaac aaaacgtccg
    23701 gcacgagaca taaattcttc ttcgctcatc tcctgtccgt taaaaacaac tttacccggt
    23761 gtataacgca gttgcagcga ggcggtattg ttgtcaatcg tgattaactg aaacatctga
    23821 cctaaggtca tcatcccgct gatttgttta tcagcccgct tttgcgcttt ttccgcatcc
    23881 attccttcag ataaattaag ctgttttgcg gtttctgtga cgacattaag cggtaacttc
    23941 acatcaaaat tgagcgattt aatatctttg tttgtggatg atgaagattt ggctgggtcg
    24001 gcaatactga tatccagatt ggcatttagt tcgccgagtg cgttcttcca ttttaccggt
    24061 tgtttaatgg tcggctcact tttttgtaat aacggtaaat actctttgaa caatgcagca
    24121 ttcacttctt gcagagcaac ttcatcgttt gctaactcag ggtgtgcagc aagctgcttc
    24181 tgcattgcaa tgttatactg gatgataaat tggcgcaccg ctgagggatc aatagattca
    24241 gcaatcaatg accactcacc actaccgagt tgctgattcc cttttgttaa cttatcaagt
    24301 tcgtaggtca gattgacatt gatataatct ttgtctttaa cgcgatccag catggttttt
    24361 gcaacgaaag catcgatttg ggcaacgtct tccccgtgat tgataatgtt aatctgatta
    24421 aatttcgact cgctttcacc aaccggaaat ttcttttctt ctaatcgcgc cagagaatca
    24481 atggtaaaac tttttgccgt tactttgttc attggtgata attgcagaac aatatcttcg
    24541 actttgcctt caatatccag gttagacaat gaactaccat caccattaaa agtaaactgg
    24601 ccctcaccaa aagaaaactt tccatactct gcaggggcaa catctaatgt cgtagagaat
    24661 gagccaccaa acgcgaatcg caatgtcgct tccacgggcg atttattttt cgcggcgata
    24721 aatagcggtt gtgtcagttc attattcact aagttcactt ttgccgctgc cagtgctggg
    24781 atgacattac catgcattaa cattgtgatg ggcaacggac cgtgctcaac atccacatca
    24841 aatacaactt tctggcctgg cttgatattg agatcgggtg caccgttatc gaatgtcatt
    24901 tgcatttgaa aacgggaatt aaaaaagcct ttgtcaaagt ttttataact taacgtcata
    24961 tcgtaggcat gttcaccttt ctttttcgca tcgttaaaat ctttaataaa cttttcgata
    25021 ccaggctgaa tttgcgtacc tgtgtaccat gtgccaccac cccagattac ggctaacgca
    25081 acaataacac ctgtagctga cttacgtatc atataaatat tccctgataa aacagcaaaa
    25141 ctttgaatat ccacttatgc tgaaataatg tccagcggca ggatgcatta tcagaatgta
    25201 tttatattta ttaaataata aaaaaagccc gtgaatattc acgggcctta tgtaatttac
    25261 attgaattat tttttctcgg acagatattt cactgtatca gcatactgct gaacaaaaac
    25321 atccatattg ctggtatcca taccctgcgg attcagctga tatttaccgt taacaaacat
    25381 cgccggaacg ccacgcaatt gcacgtcagc tgcagctttt tcctgctgag cgaccagaga
    25441 tttgaccacg aagctgttcc acgccgcgtc gtactcttca cctttaatac ctgcgttgat
    25501 aaatacatcg cggatatcag atgctgaacg aatggtctgg gttttctgta cgccttcaaa
    25561 cagcggaaca gtgactttgt cttccacgcc cagcgccatc gccacagccc atgcctgagt
    25621 cagatctttg cccaggtcac cacccatgaa gttgacgtgg tatttagtca tcttcacgcc
    25681 ttccggcagt tttttcttca cattatcaga aatatgcaga acttcttcaa actgatagca
    25741 gtgcggacag aagaaagaga aaaactccag cacttgcggc gcgccagcta ccggtttttc
    25801 cagggtagtg tactgtttac catcttcata ctgcgccgcc gatgcgctaa acgctaaaac
    25861 taaaccagcc agcgccagcc aaatcttttt catgatctac tctctccgat taatacatag
    25921 gtgttaattg taaagggggt tcttgtagaa cttttgcctg ttctataaaa gtcgccgttt
    25981 gtcgcagcca gtaatcttcc ccggttaacc acgggaaatt tttcgggaac gcgggatcag
    26041 cccaacgccg cattagccag gcaagataat aaaccaaacg catggcgcgt aaaggttcaa
    26101 tcagtccgat ttcagcggtg tcgaactcgc taaattcttc ataagcttca ataatagttt
    26161 ccagttgcat ccgctgctcg gctttatcgc cattgagcaa catccacaaa tcctgaatag
    26221 ctggaccatt acgtgcatca tccagatcaa caaacattgg accatcgcgc cagagaatat
    26281 tcccggcgtg gcagtctcca tgtagccgca gaacggtgaa atcttcccgc cagtgcgctg
    26341 taacggcggc gatcagctca tctgtcgctt tcaggaatgc cgctttcaac ccggaaggta
    26401 tcagtgtagc gtcctcaaac agcttgcgtg gctcaatgag atattcattc agaccgatgg
    26461 tcgggcgatg gataaaaagc tgtttgcgcc ccgtctggtg catacgccct aaataacgcc
    26521 caaccgcttc catctgatcg atattatcag cttcgaactg gcgaccaccg acgcttggaa
    26581 aaacagcgaa ataaaatccc tgatgattca ataaagtctg accgttaaag gccacaggtg
    26641 ctgcgaccgg aacttcatca tttaccaact gcaacgcaaa ttgatgttct tcgaggattt
    26701 gatcggctgt ccaacgttca gggcgataaa atttgacgac aaaacgtcga cgatcttcgt
    26761 cctgaaattg atagacacgg ttttcatagc tgttaagcgg ggtaagaccg gaatccaccc
    26821 ggatcccttg ctcaaataga gcatccatga tggtatccgg gtgtagtgtc tggaaagtaa
    26881 aagcgctgtt gttcatccca tcatccggaa aacattacga atgatgaagg atatcatttc
    26941 acgatgattt tggtggcacc gcttacaagc ttttactctt taattacgcc gcgagcgcgt
    27001 aacagcgccg ttttgaagtc ttcttcataa tctttctgca aacccgggat caccgcatct
    27061 ttcgcggaat cacgcatttt caggtgatag atcagaatgt catccgtcaa atccgccagt
    27121 tcgccgtcaa aacctgactc tttcgccagt ttctgcaaaa attgcaacag gttaaggtct
    27181 ggctcttttt gccaggctgg ctggaggagt tcaataactt cattcagacg tttacatttc
    27241 atagtaatgc tccttactct tgagacagac acgttagcag ggtcaatccc acaataaaag
    27301 aggcgatatc ggtgaatctg atgacgacga taacaggcgt tgtgctggca ggcggtaaag
    27361 ccagacgaat gggcggcgta gataaaggat tgcttgaatt aaacggcaaa ccattatggc
    27421 aacatgtcgc tgacgcgctt atgacgcagc tctctcacgt cgtggttaat gccaatcgcc
    27481 atcaggaaat ctatcaggca agcggtctga aagtgattga agattcactg gcggattacc
    27541 caggccctct ggcaggaatg ctttcagtaa tgcagcagga agcaggcgag tggtttttgt
    27601 tttgtccgtg cgatactcct tatattcccg aggatttagc agcccggctt aatcatcagc
    27661 gcaaagatgc gcctgtcgtg tgggtccacg acggtgaacg cgatcacccg actattgctc
    27721 tggtaaaccg cgcaattgag cctttattac tggaatatct gcaagcagga gaacgccggg
    27781 taatggcatt tatgcgtctg gctggcggtc atgcggttga tttcagcgat aataaagatg
    27841 catttattaa cgtgaatacg ccagaggagc ttgcccgatg gcaggaaaaa cgatgatacc
    27901 gttactcgcc tttgccgcgt ggagtggtac cggaaaaact acgcttttga aacaactgat
    27961 cccggcatta tgcgccagag ggatccgtcc agggctgatt aagcatacgc accatgatat
    28021 ggatgttgat aagccaggca aagatagcta tgagctgcgc aaggctggcg cggcacaaac
    28081 catcgttgcc agccagcaac gatgggcctt gatgacggaa acaccagacg aagaagagct
    28141 ggatctacag tttctcgcaa gtcgaatgga tacctcaaag ctggatttga ttctggtcga
    28201 agggtttaag catgaagaga tcgcaaagat tgtgctgttt cgcgatggag ccggacatcg
    28261 acctgaagaa ttagtgatag acaggcatgt tattgctgta gccagtgatg tgccgcttaa
    28321 tcttgatgtc gcattactgg atatcaatga tgttgagggg ctggctgatt ttgtggtgga
    28381 gtggatgcaa aagcagaacg gataagcaag agtaggctgg ataagacgcg ccagcgtcgc
    28441 atccggcatt tttttctgcg ccaatgcaaa aaggccatcc tgacggatgg ccttctgctt
    28501 aatttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
    28561 cgcaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
    28621 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgttt
//
LOCUS       DAZGOB010000054        28476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGOB010000054 DAZGOB010000000
VERSION     DAZGOB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.054"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            128..670
                     /gene="zapC"
                     /locus_tag="ACHLIF_002517"
     CDS             128..670
                     /gene="zapC"
                     /locus_tag="ACHLIF_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO9606513.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(667..1776)
                     /gene="ycbX"
                     /locus_tag="ACHLIF_002518"
     CDS             complement(667..1776)
                     /gene="ycbX"
                     /locus_tag="ACHLIF_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO9606514.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PGAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            2020..4128
                     /gene="rlmKL"
                     /locus_tag="ACHLIF_002519"
     CDS             2020..4128
                     /gene="rlmKL"
                     /locus_tag="ACHLIF_002519"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO9606515.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4140..6047
                     /locus_tag="ACHLIF_002520"
     CDS             4140..6047
                     /locus_tag="ACHLIF_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9606516.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6177..7430
                     /gene="pqiA"
                     /locus_tag="ACHLIF_002521"
     CDS             6177..7430
                     /gene="pqiA"
                     /locus_tag="ACHLIF_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO9606517.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7435..9075
                     /gene="pqiB"
                     /locus_tag="ACHLIF_002522"
     CDS             7435..9075
                     /gene="pqiB"
                     /locus_tag="ACHLIF_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO9606518.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9072..9635
                     /gene="pqiC"
                     /locus_tag="ACHLIF_002523"
     CDS             9072..9635
                     /gene="pqiC"
                     /locus_tag="ACHLIF_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO9606519.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASAWSQEAASIAQEIKRLP"
     gene            9891..10058
                     /gene="rmf"
                     /locus_tag="ACHLIF_002524"
     CDS             9891..10058
                     /gene="rmf"
                     /locus_tag="ACHLIF_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO9606520.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10128..10646)
                     /gene="fabA"
                     /locus_tag="ACHLIF_002525"
     CDS             complement(10128..10646)
                     /gene="fabA"
                     /locus_tag="ACHLIF_002525"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO9606521.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10715..12475)
                     /locus_tag="ACHLIF_002526"
     CDS             complement(10715..12475)
                     /locus_tag="ACHLIF_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9606522.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLSDFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12661..13113
                     /gene="matP"
                     /locus_tag="ACHLIF_002527"
     CDS             12661..13113
                     /gene="matP"
                     /locus_tag="ACHLIF_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO9606523.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13188..14228)
                     /gene="ompA"
                     /locus_tag="ACHLIF_002528"
     CDS             complement(13188..14228)
                     /gene="ompA"
                     /locus_tag="ACHLIF_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO9606524.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14585..15094)
                     /gene="sulA"
                     /locus_tag="ACHLIF_002529"
     CDS             complement(14585..15094)
                     /gene="sulA"
                     /locus_tag="ACHLIF_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO9606525.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15313..15942
                     /gene="sxy"
                     /locus_tag="ACHLIF_002530"
     CDS             15313..15942
                     /gene="sxy"
                     /locus_tag="ACHLIF_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO9606526.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15905..18067)
                     /gene="yccS"
                     /locus_tag="ACHLIF_002531"
     CDS             complement(15905..18067)
                     /gene="yccS"
                     /locus_tag="ACHLIF_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO9606527.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18077..18523)
                     /locus_tag="ACHLIF_002532"
     CDS             complement(18077..18523)
                     /locus_tag="ACHLIF_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO9606528.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18646..20700
                     /gene="helD"
                     /locus_tag="ACHLIF_002533"
     CDS             18646..20700
                     /gene="helD"
                     /locus_tag="ACHLIF_002533"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO9606529.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20732..21190)
                     /gene="mgsA"
                     /locus_tag="ACHLIF_002534"
     CDS             complement(20732..21190)
                     /gene="mgsA"
                     /locus_tag="ACHLIF_002534"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO9606530.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21286..21948)
                     /locus_tag="ACHLIF_002535"
     CDS             complement(21286..21948)
                     /locus_tag="ACHLIF_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO9606531.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22121..22534
                     /locus_tag="ACHLIF_002536"
     CDS             22121..22534
                     /locus_tag="ACHLIF_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO9606532.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22579..22896)
                     /gene="hspQ"
                     /locus_tag="ACHLIF_002537"
     CDS             complement(22579..22896)
                     /gene="hspQ"
                     /locus_tag="ACHLIF_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO9606533.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22954..24144)
                     /gene="rlmI"
                     /locus_tag="ACHLIF_002538"
     CDS             complement(22954..24144)
                     /gene="rlmI"
                     /locus_tag="ACHLIF_002538"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO9606534.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEIFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24239..24517
                     /gene="yccX"
                     /locus_tag="ACHLIF_002539"
     CDS             24239..24517
                     /gene="yccX"
                     /locus_tag="ACHLIF_002539"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO9606535.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24514..24843)
                     /gene="tusE"
                     /locus_tag="ACHLIF_002540"
     CDS             complement(24514..24843)
                     /gene="tusE"
                     /locus_tag="ACHLIF_002540"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO9606536.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24934..25593)
                     /gene="yccA"
                     /locus_tag="ACHLIF_002541"
     CDS             complement(24934..25593)
                     /gene="yccA"
                     /locus_tag="ACHLIF_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO9606537.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(26001..27020)
                     /locus_tag="ACHLIF_002542"
     CDS             complement(26001..27020)
                     /locus_tag="ACHLIF_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9606538.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26998..27240)
                     /locus_tag="ACHLIF_002543"
     CDS             complement(26998..27240)
                     /locus_tag="ACHLIF_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO9606539.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(27308..>28476)
                     /locus_tag="ACHLIF_002544"
     CDS             complement(27308..>28476)
                     /locus_tag="ACHLIF_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO9606540.1"
                     /translation="ARKNGTYTDLPQNVDTAEDSVQPEPVKVTADEVNKIMQAANISQ
                     PDADKLLAASRGEFVEGISDPNDPKWVKGIETCDSVNQNQQESEQNDQKTEQNSPNAQ
                     QNEPETKQEPEKACTACGQTGGGSCPDCGAVISDATYQEIFDEENQPEVQENDPEEME
                     GTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNITSTSRAGIHLMIDLETMGKN
                     PDAPIISIGAIFFDPQTGEMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAILT
                     DEIPLDDALLQLREFIDENSGEFFVQVWGNGATFDNVILRRSYERQGIPCPWRYTNDR
                     DVRTMVALGLVMDFDARTTIPFEGERHNALNDARYQAKYVSAIWQKLLPSQADF"
BASE COUNT         7162 a         6946 c         7403 g         6965 t
ORIGIN      
        1 cagccctggt tgttttatat tcatcgctgt tgcttattta gacattttgt acttttattg
       61 ttgaggttat taagcgaagc gacaatggat tgtgttgctg cggttttata gggtagggga
      121 gaggcagatg cgaattaaac cagacgataa ctggcgttgg tattacgatg aagagcacga
      181 tcgaatgatg ctcgatttag ccaatggtat gctatttcgc tcacgttttg cgcgcaagat
      241 gttgactcca gatgcctttt ctcccgcagg cttttgcgtt gacgacgccg cgctctattt
      301 ctcttttgaa gaaaagtgcc gcgattttaa tttatctaaa gaacaaaaag ccgaactggt
      361 gctgaatgca ctggtagcaa ttcgctatct caaaccgcaa atgccgaaaa gctggcattt
      421 tgtttcccat ggtgaaatgt gggtgcccat gccgggcgat gcggcctgcg tttggttgag
      481 cgatactcac gagcaggtca atttgctggt cgttgaatct ggcgaaaatg ccgcactatg
      541 cctgctggca caaccctgcg ttgttattgc gggtcgcgcg atgcagttgg gcgacgccat
      601 taaaatcatg aacgacaggc tgaaaccgca ggttaatgtt gacagcttca gcctcgaaca
      661 ggcagtctaa cgcgccaact taagtgcagt cttcggtaca cagctacagc aaagaatggt
      721 gccatcatcg cccattgctg atttttttag cggcgtgact tcgccttcta aaagctgaac
      781 acggcaactt ccacaaatgc ccgcgcggca agaataaggg atacgaattc cctgattttc
      841 taattgctcc agcaacacct gttggttatt tccacgaaat gcctgtccct gccagtcaat
      901 atctacattt gcgtccggtt gttgcgtgat gttggcggta tcatcagcgg cagctgcgcc
      961 gtaaattttt gccggagccg ttgccagaat ttccacctca tcgccaacgc gaatcacgcc
     1021 gctattacgg gcaattaaat tctgaccaaa atcgacatcg ccgttatcct gggcagtgcg
     1081 gaaagattgc aatgttttta atggttcgcc tgccggatgt ttttgccctt tttctgggct
     1141 gacggtggtg aaaatacagc ggctacaagg tttaaccaca tcaaacacca catcaccaat
     1201 gcgaatcact ttccagcgat cttcttccca ggctgacgcg ccggaaacca ccagattggg
     1261 gcggaattgc tccattttta cactggccgg acaacgttgt tggagatcac gtaacgaggc
     1321 ttcgttagca agaaggtaag gatagccatc agcaaatgac agaggtacag tgttgtggcg
     1381 tttcacgcgc cgggtcattt gtggccccac ccagcgtaat tgcacttcgc gggagaaaaa
     1441 tccacttagc catttgttga tcgcgcctgg cgcaattcgc gcggtaaaat gtgtgcccca
     1501 aacttcggtt ggtgcgtctt gtgtggcgaa atcagcaaaa cgaacatatg cgctactgcc
     1561 atctggtgcg gtgagatgca agccatcatg cacaggtgaa ggagtaaacc gtaccatttg
     1621 cggaaactgg cgagcagtaa taaacgtacc atcaggttcc gtgatcataa agatgcgatc
     1681 gaaggccaga ccactcacat ctgccagggt atgtgtaaga ccaatgccgc gcatcgattt
     1741 aacaggatga ataaaaagcc gggttaatgt cgccacggtg cggtcctcaa atgaaaataa
     1801 gccctcaact ttatgacatg acgcgcttat tagctataat gcgcaacaat tttcttagcg
     1861 cctgtcccgt taggctattt tatttgtcag tttggcccag ggaagtgctc gaaatcctca
     1921 cgtacttgtg tacgctccgg tttctccgcg ctgtccatgt ccagactgac tgcaacaatt
     1981 acgcctactg cggtaggctc ttagagtaaa agtgacgata tgaattctct gtttgccagt
     2041 acggcccgtg ggctggaaga gctgttaaaa actgaactgg aaaacctggg ggccgttgaa
     2101 tgccaggtgg ttcagggtgg ggtccatttc aagggcgaca cacggcttgt ttaccagagc
     2161 ctgatgtgga gccgcctggc ctcgcgtatt atgttgccgc tgggcgagtg taaggtttac
     2221 agcgatttag acctctatct cggtgttcag gcgatcaact ggacagagat gtttaatcct
     2281 ggcgcgacct tcgctgtcca cttcagtggt ttgaatgaca ccatccgcaa cagtcagtac
     2341 ggcgcgatga aagtgaaaga cgcgatcgtc gatgctttca cgcggaaaaa tctgccgcgt
     2401 ccaaatgttg atcgcgatgc gccggatatc cgcgttaacg tctggctgca taaagaaacc
     2461 gccagtatcg ctcttgatct gagtggtgat ggtttacatc tgcgtggcta tcgcgatcgt
     2521 gctggtattg cgccgatcaa agaaaccctg gcagccgcta ttgtgatgcg atccggctgg
     2581 cagccaggaa caccgctgct cgatccgatg tgtggttccg gtacgttgct gattgaagcg
     2641 gcgatgctgg cgaccgatcg cgcaccaggc ttgcaccgtg ggcgttgggg ctttagcggc
     2701 tgggcgcagc atgatgaagc tatctggcag gaagtgaaag cggaagcgca aactcgcgcc
     2761 cgtaaaggcc tggctgagta tagctctcat ttttacggtt cggacagcga cgcacgggtg
     2821 attcaacgtg cacgcactaa cgcccgtctt gcggggattg gtgaactgat cacctttgag
     2881 gtgaaagatg tcgcgcaact gaccaatccg ctgccgaaag ggccgtacgg cacagtgttg
     2941 agcaacccgc catacgggga acgtctggac agcgaaccgg cgctgattgc gctgcatagc
     3001 ctgctgggtc ggatcatgaa aaaccagttt ggtggctgga atctctcttt gtttagtgcc
     3061 tcgccggatc tgctaagctg cttgcagctg cgtgcagaca aacagtacaa ggcgaaaaac
     3121 ggcccgctgg actgcgtaca gaaaaattac cacgttgccg aaagcactcc agacagcaaa
     3181 ccggcgatgg tagcggaaga ctacgccaac cgcctgcgta agaacctcaa aaaattcgag
     3241 aagtgggctc gccaggaagg aattgaatgt taccgcctgt atgacgccga tctaccggaa
     3301 tataacgttg ccgttgaccg ttatgccgac tgggtggtgg tgcaggagta tgcgccgcca
     3361 aaaactattg atgctcacaa agcgcgtcag cgtctgttcg atattatcgc tgcaaccatt
     3421 tcggtactgg ggattgcgcc aaacaaactg gtgctgaaaa cccgtgaacg ccagaagggc
     3481 aaaaatcagt accagaaact gggcgagaag ggcgagtttc ttgaagttac cgaatataac
     3541 gctcacttgt tggtgaacct gacggattac ctcgataccg gtctgtttct cgatcaccgt
     3601 atcgcccgtc gtatgctcgg ccagatgagc aaaggcaaag atttcctcaa cctgttctct
     3661 tataccggca gcgccaccgt gcatgcggga ttaggcggtg cacgcagcac caccaccgtg
     3721 gatatgtcgc gtacttatct ggagtgggca gaacgcaacc tgcgtctgaa tggcctgacc
     3781 gggcgtgcgc atcgcctgat tcaggccgat tgcctggcgt ggctgcgtga ggcaaatgaa
     3841 cagttcgatc tgatctttat cgatccgcca accttctcta actcaaaacg aatggaagat
     3901 gcgtttgatg ttcagcgcga tcatctggcg ctgatgaaag atttgaaacg tctgctgcgt
     3961 gcaggtggga cgatcatgtt ctcgaacaac aaacgtggct tccgtatgga tctcggcggc
     4021 ctggcaaaac tgggactgaa agcacaagaa attacgcaaa aaacgctctc ccaggatttc
     4081 gcccgtaacc gccagatcca caactgctgg ctgattaccg cagcctgaaa ggaatagtaa
     4141 tgtcattaat cagtatgcat ggcgcctggc tgtcgttcag cgacgcgccg cttctcgata
     4201 acgcagaact gcatatcgaa gataatgaac gtgtttgtct ggtaggccgc aacggcgcag
     4261 gcaaatcgac gttaatgaaa atcctcaacc gtgaacaagg gctggatgac ggtcgcatta
     4321 tttacgagca agatttgatt gtagctcgtc tgcaacagga tccgccgcgt aacgttgagg
     4381 gtagcgttta tgatttcgtt gccgaaggca ttgaagaaca agcggaatat ctgaaacgct
     4441 atcacgatat ttcgcgcctg gtgatgaacg acccgagcga gaaaaatctc aacgaactgg
     4501 cgaaggttca ggaacagctg gatcaccaca acttgtggca gctggaaaac cgcatcaacg
     4561 aagtgctggc gcaactgggg ttagatccta atgttgcgct gtcgtcgctt tccggcggct
     4621 ggttgcgtaa agcggcatta ggacgcgcgc tggtgagcaa tccgcgcgta ttgctgctgg
     4681 acgaaccgac taaccatctg gatatcgaaa ccatcgactg gctggaaggg ttccttaaaa
     4741 ccttcaatgg gacgattatt ttcatctccc acgaccgttc gtttatccgc aatatggcga
     4801 cgcgcattgt tgatctcgat cgcggcaagc tggtgaccta tccggggaat tacgaccagt
     4861 acctgcagga aaaagaagaa gccctgcgcg tggaagaact acaaaatgcc gagttcgatc
     4921 gcaaactggc gcaggaagaa gtgtggattc gccaggggat caaagcacgc cgtacccgta
     4981 atgaaggccg cgtacgcgcc ctgaaagcaa tgcgtcgcga gcgtggtgaa cgtcgcgaag
     5041 tgatgggtac cgcaaagatg caggtggaag aggccagccg ctccggtaag atcgttttcg
     5101 aaatggaaga cgtttgctac caggttgacg gtaagcaact ggtgaaagat ttttcggccc
     5161 aggttctacg tggcgacaaa attgccctga ttggtccgaa tgggtgcggc aaaaccacgc
     5221 tgctgaaact gatgctcggt cagcttcaag cggacagcgg gcgtattcac gttggcacca
     5281 aactggaagt ggcttatttc gatcagcacc gcgcggaact ggatcccgat aaaacggtga
     5341 tggataacct tgccgaaggt aagcaagagg tgatggttaa cggcaagcca cgccacgtat
     5401 tgggctattt gcaggacttt ctgttccatc cgaaacgggc gatgacgccg gtacgtgcgc
     5461 tttctggcgg tgagcggaac cgcttgctgc tggcgcgttt gttcctcaaa ccaagcaact
     5521 tattgattct tgacgaaccg accaacgatc ttgatgtcga aacgctggaa ctgctggaag
     5581 aactgatcga cagctatcag ggcacggtat tgctggttag ccacgatcgt cagtttgtcg
     5641 ataacaccgt tacagaatgt tggatcttcg aaggcggcgg taaaattggt cgttatgtcg
     5701 gcggttatca tgatgcccgt ggtcagcaag agcagtatgt ggcgctcaaa cagcctgcgg
     5761 tgaaaaaaac cgaagaagcc gctgcggcaa aagcagaaac tgtaaaacgc agcagtagca
     5821 aactaagcta taaattgcag cgcgaactgg agcagctacc gcaattgctc gaagatctgg
     5881 aggcgaagct ggaagcccta cagacgcagg tggcggatgc ttccttcttc agtcagccac
     5941 atgagcagac acaaaaagtg ctcgccgata tggctgctgc agagcaggag ctggagcaag
     6001 cctttgaacg ctgggagtat cttgaagcgt taaaaaatgg tggctgatcg caaaatagtc
     6061 gatttaaaca gagacagctg acaacggtaa aattgcctga tgaactgggt ttatcaggca
     6121 tgaaaaccaa cactttgtta gtcatctgta aaatagcgca tcattaagga gtaccaatgt
     6181 gcgaacatca tcatgccgcg aagcacatcc tgtgctcgca gtgtgacatg ctggtggcgt
     6241 taccgcgcct tgagcatggt cagaaagcgg catgtccccg gtgtggcaca acgttaaccg
     6301 tggcgtggga tgcccctcgg cagcgtccca ccgcctatgc gttggctgca ctgttcatgc
     6361 tgttgctgtc caacttgttt ccttttgtga atatgaacgt tgcaggagtt accagtgaaa
     6421 ttacattact ggaaattccc ggcgtgcttt tttctgagga ctacgccagc ctcggcacct
     6481 ttttcctgtt gtttgtgcaa ctggttcccg cgttttgtct gataaccatt ctgttactgg
     6541 tgaatcgcgc ggaattaccg gtccgtttaa aagagcaact ggcacgggtg ctttttcaac
     6601 tcaaaacctg gggaatggcg gagattttcc tcgctggtgt gctggtcagt ttcgttaaac
     6661 tgatggctta cggcagcatt ggggtaggca gcagctttct cccctggtgt ttattttgtg
     6721 tcctgcaact gcgcgctttt cagtgcgttg atcgtcgctg gttatgggac gacatcgccc
     6781 cgatgccaga actgcgccag ccgctaaaac caggcgtcac ggggatacgt caggggctgc
     6841 gttcgtgctc ctgttgtacg gcaatccttc ctgctgatga acccgtgtgc ccgcgttgta
     6901 gtaccaaagg gtacgttcgg cgtagaaaca gcctgcagtg gacactcgcg ctgcttgtaa
     6961 cgtccatcat gctgtatctt ccggctaata ttttgcccat catggtgacg gatttattag
     7021 gctcgaagat gccgtcgacg attctcgctg gggtcattct gttatggagc gaaggctctt
     7081 atcccgtcgc tgcggtgatc tttctggcca gtattatggt gccaacgtta aagatgatcg
     7141 ccatcgcgtg gctgtgttgg gatgccaaag ggcatggcaa gcgcgacagt gaaagaatgc
     7201 atttgattta tgaagttgtt gagtttgtag gccgctggtc gatgattgac gttttcgtta
     7261 tcgcggtgct ctcggcgctg gtgcgtatgg gaggtttaat gagtatttat ccggcaatgg
     7321 gtgcattaat gtttgcttta gtcgtcataa tgacaatgtt ttctgctatg acgtttgacc
     7381 cgcgtttgtc gtgggatcgt caacctgaat cagagcatga ggagtcctga cagtatggaa
     7441 tctaataatg gggaagccaa aatccagaaa gtgaagaact ggtctcccgt gtggatattt
     7501 cctatcgtca cggcgctcat tggggcctgg gttctttttt atcattacag ccatcaggga
     7561 ccggaagtga ccctgatcac cgcgaatgcg gaaggaattg aaggtggcaa aaccaccatt
     7621 aaaagccgta gcgttgacgt cggcgtggtt gaaagcgcca cactggctga tgatttgacg
     7681 cacgttgaaa tcaaagcgcg gctgaattcc ggtatggaaa aattgctgca taaagacacc
     7741 gtcttttggg tggtgaaacc gcagattggt cgagaaggga ttagcggtct gggaaccctg
     7801 ctgtctgggg tttatatcga actgcagcca ggcgcgaaag gcagcaaaat ggataaatac
     7861 gatttgctgg actcgccacc gttggccccg cctgatgcga aaggtatccg tgtgattctc
     7921 gatagcaaaa aagccgggca gctctcgcca ggagatccgg tgctgttccg tggctatcgg
     7981 gtaggttcgg ttgaaaccag caccttcgat acacaaaaac gcaatatcag ctatcaactg
     8041 ttcatcaatg caccttatga ccgactggtg accaacaatg ttcgcttctg gaaagatagt
     8101 ggcattgcgg ttgatctgac gtcagcaggg atgcgtgtgg agatgggctc attgacaacg
     8161 ctgctgagtg gcggtgtcag ctttgatgtg ccggaaggtc tggatttagg gcagccagtg
     8221 gcaccgaaaa cagctttcgt tttgtatgat gatcagaaga gcattcagga ttcgttgtac
     8281 accgatcaca ttgattatct gatgttcttt aaagattcgg tacgcggtct gcaaccggga
     8341 gctccggtag agttccgggg tattcgcctg ggtaccgtaa gcaaagtgcc attctttgcg
     8401 ccgaatatgc gtcagacatt taacgatgat taccgtattc cggtactgat tcgtatcgag
     8461 ccagagcggc tgaaaatgca gcttggcgaa aatgcggatg ttgttgagca ccttggcgaa
     8521 ttgttgaaac gtggtttacg cggatcgctg aaaaccggaa acctggtcac tggtgcactg
     8581 tatgttgatc tcgatttcta tccaaatacg cctgcaataa ccggtattcg tgaatttaat
     8641 ggttatcaga ttatcccgac cgttagcggc ggcctggcgc aaatccagca acgactgatg
     8701 gaagcgttgg ataagatcaa caaactgcca ttgaatccga tgattgaaca ggcaaccagt
     8761 acgctttctg aaagtcagcg cacaatgaaa aacctgcaaa cgacgctgga tagcatgaac
     8821 aagatcctcg ctagccagtc gatgcagcag ttgccgacgg atatgcagtc aacgttgcgt
     8881 gaattgaatc gcagcatgca gggcttccag cctggctccg cagcctacaa caagatggtg
     8941 gcggatatgc agcgccttga tcaggtgttg cgagaactgc aaccggtgct gaaaacgctc
     9001 aatgagaaga gtaacgcgct ggtatttgaa gcgaaggaca aaaaagatcc agagccgaag
     9061 agggcgaaac aatgaaaaag tggctagtga cgattgcagc actgtggctg gccggatgca
     9121 gctccggcga aattaataaa aactattacc agttacctgt ggtgcagagc ggtacacaaa
     9181 gtaccgccag ccagggcaat cgtctgttat gggtagagca ggtcactgtt cctgactatc
     9241 tggcggggaa tggtgtggtt tatcaaacca gtgatgtgaa gtatgtgatt gccaataaca
     9301 acttgtgggc cagcccgttg gatcaacagt tgcgcaacac cctggttgcc aacctgagta
     9361 cgcaactgcc cggctgggtg gttgcctccc agcctctggg aagcgcccag gacacgctca
     9421 atgttaccgt cacggagttt aacggtcgct atgatggcaa ggtcattgtc agtggtgagt
     9481 ggctgttgaa ccaccaggga caactgatca aacgtccgtt ccgtctggaa ggggtgcaaa
     9541 ctcaggatgg ttacgatgag atggttaaag tactggccag tgcctggagt caggaagccg
     9601 cttctattgc gcaagagata aagcgtctac cttaattata aagatttgta aatataaccg
     9661 cctccggtat gttgcctgag gcggtttttt tgtctctaac gtgcggaaaa agttgttcct
     9721 cttcacattt tttgtacaac cggcataccc gtgtagctca caaatatgac agtggcgtga
     9781 attttgcgca ttgacggcag ttatgattcg cggtattgct taactgtgat tgcacattta
     9841 gtaatcactg ttttcttttc caccagaaac cagtatgagg gaaacgaggc atgaagagac
     9901 aaaaacgaga tcgcctggaa cgggcacatc aacgtggtta tcaggccggc atcgccggac
     9961 gctcaaaaga aatgtgtccc tatcagacgc tgaatcaaag gtcacaatgg ctgggaggct
    10021 ggcgagaagc catggcggac agggtagtaa tggcctgatt ctgtctcttt aaaaagaaac
    10081 ctccgcattg cggaggtttc gccttttgat actctgtctg attataatca gaaggcagac
    10141 gtatcctgga acagaccgac tttcaggtcg ctggcggtat agatcagacg accatcaacc
    10201 agcacttcgc catccgccag gcccataatc agacgacggt taacaatgcg tttaaagtga
    10261 atacggtagg tcactttttt cgctgtcggc agtacctgac cagtgaattt cacttcgcca
    10321 acgcccagcg cgcggccttt accttcgccg cccagccagc cgaggtagaa ccctaccagc
    10381 tgccacattg cgtccaggcc caggcatccc ggcataaccg gatcgccaat aaagtggcat
    10441 ccgaagaacc acagatccgg attgatatcc agttctgctt caacataccc tttgtcgaag
    10501 ttaccacccg tttcggtcat tttgaccaca cggtccatca tcagcatgtt cggtgctggc
    10561 aattgcgggc ctttagcgcc aaacagttca ccgcgaccag aggcaagaag gtcttctttt
    10621 gtataggatt cgcgtttatc taccatgttc tctgtaagcc ttattttatt gaagcacgca
    10681 ggatagctaa cacgtgtacg ctgaacaagt ccgatcagtt cggaataaac cagttcagcc
    10741 aacgtaatgg ccatggaaaa cggtgacgtc cttcctgttg cgatgcttgc gcgatacgtt
    10801 cctggatggt ttgcatcagc gtcgtttggc cttcgccatc ccacaccaga tttaataata
    10861 acggcagtgc gtcagtcaca tcgtctactg cccagatggt gaatttgcct tcttctaccg
    10921 ctttcaccag ttcactgtga agacttaaat ggcgaacgtt agctgtgggg ataatgacac
    10981 cttgtttccc ggttaactca cgttgctggc aaatagcaaa gaagccttcg attttctcat
    11041 ttaaaccacc gaccggctgg gcgcgaccga actgatcgac tgaacctgtg atagcgatac
    11101 tctgattcac cggcacatcg gcgagggcgc ttatcagggc gcagagttca gccatcgagg
    11161 cactatctcc atcaacttca ctgtatgact gctcaaatgt cagcgatgct gagaagggga
    11221 tctgttgctc aagctgtagt tccgacatca ggaacgcttg catgatcatc atccctttcg
    11281 catggatatt gccgccaagc tccgctttgc gttcgatgtc ggtgaattca ccatcgccaa
    11341 tatgcacaac gcagctaatg cgagaaggtt cgccaaaagc gcgtggatga cccggaaatt
    11401 caatgaccga aagggcgtta atttgcccga tgcgttcgcc ttcggtttca atcaggattt
    11461 gctcctgaag gatctcatcc tgcatacgtt cagcgaggaa accttcgcgc cattcacgct
    11521 gctgcagcat taagtttagc tgctcgccgg agaaggtgtc gccatcacac agggaggcga
    11581 cctctttaca ctggcggagg atccactgcg ggctaagcgg aagtgtttct tgttcaccgg
    11641 tgtagcgtgc tgcttcgcgg ataagtaccg gccaggcatc cgctcccggt gcaggtaagt
    11701 gattatgtct ggcggtaaat gtcacccagc gacaccactg ggttactgac tctgcatcga
    11761 caatctgcag agtatcttca aattcgctat aaatagcctg ctctgaaagc tctggctcca
    11821 tctcctggaa atcagacaat gattcgcgtt cgcctaccag aatgactttc agcttcaatg
    11881 gcatcgaagg cacagagacg gggagagggc gcgactcatc aaacgcaacc cagtcaaaac
    11941 gctcgcggtt aacgatattt ttcagccgca tccacagcag aggttgcgcc agcagtgtac
    12001 gcaaagagat gatgagaata ccgccatttg cctgatgcac cagaccaggc tgcagggtaa
    12061 tgtcgccatt aaactggcgc aggcagccaa agagttgctc cgcttctacc cagtcggcag
    12121 cgacaacttg cgttaaagtc gcaaaattat catctgcact cactgcgtga cgtaagcgga
    12181 tggagtggcc ggaaacctca tagtgaccgc ccaccagttg gcctgcatcg ctttgtagcg
    12241 tacgcgcggc attggcaata agattcagat actcagactc ttccggggcc ttcgccagca
    12301 taaaagagga ggatgctcgc gtatgcagca actgctccag tgcgaattgc agtcgcggtt
    12361 gagtatcact gaataaagga tcgttttcgt caatcaaatg tggctgagca aatatttcct
    12421 gatagctatc ggtatcagga accaggtcac gccatgcaag tttcgtaatg gtcaaagttg
    12481 atgtttttta gtctgttgtc aaagccgcga ttataccgta accggcacta cagcacacgt
    12541 agaaagcacc gacaatactc ctggcatggg cgttaaagct cacaggatgg agattctttt
    12601 cttcactggc ctaaaaagct gatattctgt aaagagttac acggtaacat tgagatcgct
    12661 atgaaatatc aacaacttga aaatcttgaa agcggttgga aatggaagta tctggtgaag
    12721 aagcatcgcg aaggggagtt aatcacccgt tacatagaag ccagtgccgc ccaggaagcc
    12781 gtcgatgtgt tgctctcgct cgaaaatgaa cccgtgctgg taaatggctg gattgacaag
    12841 catatgaatc cggaactggt caatcgcatg aagcaaacca ttcgggcaag acgaaagcgc
    12901 cattttaatg cggaacacca gcatacgcgc aaaaaatcga tcgatctgga atttattgtc
    12961 tggcaacgtc tggctggtct tgcgcagcgt cgcggtaaaa cgctttctga aacgattgtt
    13021 cagctgattg aagatgcgga aaacaaagag aaatacgcga ataaaatgtc ttctctgaag
    13081 caggatctgc aggcattgct gggtaaggaa taactgacga aagtcagttc aatttactaa
    13141 aggcaaaaaa accccgcagc agcggggttt ttctaccaga cgagaactta agcttgcggc
    13201 tgagttacaa cgtctttgat acctttaact tcgatctcta cgcgacgatc cggagccagg
    13261 cagtcgatca gtgcagcacg ctgtttcacg ttgtcacagg tgttgccagt aaccgggttg
    13321 gattcgccca taccacgtgc ggagatcttg tctgccggga tacctttgga gatcaggtaa
    13381 tcaacaacag actgagcacg gcgctcggac agaccctggt tgtaagcgtc agaaccgatg
    13441 cggtcggtgt aacccagaac aactacggaa ccgtctttcg gatccaggtt gctcagctgg
    13501 ctgtacagct gatccagagc agcctgacct tccggtttca gggttgcttt gttgaagttg
    13561 aacagaacgt cagacttcag agtgaagtgc ttggtctgta cttccggtgc cggagctgga
    13621 gccggagcaa ctactggagc tgcttcgccc tgaccgaaac ggtaggaaac acccaggctc
    13681 agcatgccgt tgtccggacg agtgccgatg gtgtgtgcgt caccgatgtt gttggtccac
    13741 tggtattcca gacgggtagc gatttcagga gtgatcgcgt actcaacacc gccagcgaag
    13801 accggagaaa cgccggtgtc gtggttttta ccataaacgt tggatttagt gtctgcacgc
    13861 cataccatac cacccagacg agtgtagatg tccaggtcgt cagtgattgg gtaacccagt
    13921 ttagcggtca gttgaacgcc ctgagctttg tatgcaccgt tttcaacgct gcctttgtac
    13981 ggcatacgac ctaaccagtc gtaacccatt tcaaagccaa catacgggtt aacctggtaa
    14041 ccaccaaaag caccagcgcc cagttggttt tcatgggtcg ggccattgtt gttgatgaaa
    14101 ccagtgtcat ggtactggga ccagcccagt ttagcaccag tgtaccaggt gttatctttc
    14161 ggagcggcct gcgctacggt agcgaaacca gccagtgcca ctgcaatcgc gatagctgtc
    14221 tttttcattt tttgcgcctc gttatcatcc aaaatacgcc atgaatatct ccaacgagat
    14281 aacacggtta aatccttcac cgggggatct gctcaatatt aactctaccg atatcttcgg
    14341 cttatgccga gcacccctgg cgatgtaaag tctacaacgt agttgaaaac ttacaagtgt
    14401 gaactccgtc aggcatatga aaaaaaagtc ttgtataagg tatgtttaat cttttttgtc
    14461 agcgacaatt tacagaagaa aatcgcggaa accgcttcag acaagcctcc gcaaggaaaa
    14521 ttagtcacga ctgaaagcat tggctgggcg acaaaaaaag ttccaggatt aatcctaaat
    14581 ttacttaatg atacaaatta gagtgaattt ttagcccgga aagttgtctc gtggcgtgag
    14641 aggatgcgct taccggacgc ataataaacc ccatagcgtt accttcattt gccgcatcaa
    14701 caagttcagc atgctcttct tcagtcaaat catctgccaa ccaaccgatc accacactgt
    14761 aattgcccgt gcgtaaagcg cgaaccattg actccacagt gtggcaaggg gagagctggc
    14821 taatctgcat tactttcgtt aagggtagcc cagatgcctg aacccattcc cgactcagtt
    14881 tttgttgcgg tgttaaccag agttgccagc gcgattgctg accgagttgc tgtaacaatg
    14941 gcaacagtag aagttgcgtc atcatgggct gatcttcgcg atagacaact tcactgataa
    15001 gcccggctgt agtgttttcc gtagagacac gcgcaatttt acttgctgcg gatgagaacg
    15061 acgaagaacg atgtgcatag cctgaagtgt acataatcaa tccagcccct gtgagttact
    15121 gtatggatgt acagtacatc cagtgacaac aaagatcaac cctattttcg gaaagagcct
    15181 cgcaaatttt gtcgttggtg acgggaaaac ataaattaat cttgcccctt aagaataagt
    15241 tgcctatttt cgtagttaac ggatccgtta atgtgaatca ttcttttatg ttatgatttt
    15301 aaaaggaatt ttatgaaaag cctctcctat aagcggatct ataaatcaca agaatacctg
    15361 gcaacgttgg gcacaattga ataccgatca ttgtttggca gttacagcct gaccgttgac
    15421 gacacggtgt ttgcgatggt ttctgatggt gagttgtatc ttcgggcttg tgagcaaagt
    15481 gcacagtact gtgtaaaaca tccgcctgtc tggctgacat ataaaaagtg tggccgatcc
    15541 gttaccctca attactatcg ggttgatgaa agtctatggc gaaatcaact gaagctggtg
    15601 cgtctgtcga aatattctct cgatgcagcg ctgaaagaga aaagcacgcg caatacccgg
    15661 gaaagactga aagatttgcc caatatgtct tttcatctgg aagcgattct cggggaggtg
    15721 gggattaagg atgtacgggc gttacgtata cttggggcaa aaatgtgttg gttgcgactg
    15781 cggcagcaaa acagtctggt gacagaaaag attctgttta tgcttgaagg tgccattatc
    15841 ggcattcatg aagctgcgct cccggtggca cgccgccagg agcttgcaga atgggctgac
    15901 tctcttacgc cgaaacagga gtttcctgcg gaacttgagt aatctggcgt tgcagacgac
    15961 caatctcagg cagtaatgca atcaataatc caacttgttg tacgaccaga ggttctttgc
    16021 tgtctgcccg tggttcaagt tgctgcatcc gctgtttcag gctagctaat gcctcattga
    16081 cgcgttcttc atcagcaggt tgatgatgta acgcgtcatc aacatagcaa actgcgtcat
    16141 caagaaacgc cagaatttca ggattagtta actgctcccg gtgagcaccg agggctgaga
    16201 tatagctggt aaacgtatgg ttaaggcaca gcaaccgaaa cgcggcttcg cgaatttgcg
    16261 gggtaacgtt cggctcgctg gacatatttg ataccaccga cgccagctca gcatcacggt
    16321 tgtgtgcatc gcggcgggca atacgatacg ccagacggtt atcacgcccc tgatggtatt
    16381 gctccagtat ggcatcgaga taccgacagt tggcctctgt ggcgcgttcg agcatgcgcg
    16441 gcagattgcg aaactgccag tcaggccaga tgtagctcac tgccgcccac gcaatggcac
    16501 aaccaatcag cgtatcgatt acgcgaggta acgctacttc aaaaccttca cccagtaagt
    16561 taaaacacag tagcaccaaa agtgtgatga acatcgttgc atgagcgtat tgcacgttac
    16621 ggaaggcaaa aaagagcacg ccggtaataa ccagcagcac cagctgccct tccagtgatg
    16681 gcacaaacca cagcacagga atgccaatgg cgatacctac cagcgtacca ataatcctta
    16741 acttcaggcg gtggcgcgtg gcgttatagt ttggctggca gacaaacaaa cttgtcagca
    16801 agatccaata cccgtgatgc attccggtta tttgaatgat ggcgtagccg aagcacaaca
    16861 ccagcgacat tcttaccgca tgacggaaga gggcggattc cggcgtgaag tgacggctaa
    16921 gacgcagcca gatatcactc aacccgtgcg ggctgtcatc agcgagctca ttttcgtcat
    16981 tattatgggg tagtgcctgg gcctgttctg attcaattgt tgccagttgg gcatcaatgg
    17041 cgcgtaaatt gttcagcaaa aatcccagtg ttttgagtaa atcggcgggt gcgccgttat
    17101 cgcgcatccg ctccagcgca gcatcaatat gcgtaaaagc gcgctcaaaa tgcggatcat
    17161 gttgataagg ctgacgcaac aaaatacagc gtgacagttg ctggcacgcc tggccctgca
    17221 tcgacatcag ccgctgaaaa cggaacagca cgtcgctgtg gcgaaaatgt tcacgcaatg
    17281 tttgatactg aatatgagaa gagctggcac gctcgtgaat atcctgtgcg acaaagtaat
    17341 aatgcagcgt gcgacgcgtt ccccgttgac cacgatcgcc acgtaagcgg gtcagcagcg
    17401 agagtttcgt ctgattcaat gtcgccatca gcagaccgtt ggcgagagcc aaatcgtaca
    17461 gcggtgcctg gctttcatct tcaatatcag gatcaaacat gcgcgacttg agctcaagat
    17521 aacgcgccag ttgttcatag caacgcgcca ggttgtcctg cagcgggcgg accgggaaca
    17581 gcagatgacc aataagtgtc aggacgttgt accagacggc accggccagc agatacatcg
    17641 gctgctgata ccagtgctca tacagtgatg ttcccaacat agtgtaaatg gcgatcagca
    17701 atgcaccgaa ggcaattgtt gcatagcgtt gacccagacc gccgagcaaa atgaagccgc
    17761 tggtagagag cgttaagcca atcgcaaata gccagggcca gggaaacagc aattctactg
    17821 aggccgaggc gataaaaaag cagaacagcg taatgatgag gttacgcaaa cgtcccgcca
    17881 gtcggtcatc gagatcggtc agcgccgctg ccaccatccc cagcgttagc ggaatcgtca
    17941 gttttacatc acccagccac cacggaaacg ctgtggttcc acaaagcgca ataaaaatac
    18001 gcgcgtaata cagccaggcg ctgttccagg tatagcgttt gagcaaagga cttagcataa
    18061 aggccatatt agtggattat tcaaaacgac gacgtgcatt ggcttcacgc gcagcttgcg
    18121 ctgtttctac cgataccacg cgacgaccaa ccggccatag tgcaatagcg gcaattttaa
    18181 agttcgcaat gccgacagga atgccaatga ttgaaataca ttgtgcgatg cccgttgcaa
    18241 tgtgcatcag gcataaccac cagccaaaga aaatcagcca gaaaatattc aataccgtac
    18301 cgccagtatt cagcagtaca tttttgccag ccgggttcag ttcatcgaca tgaatagctt
    18361 cattgccata aggcaccaga gacagtttag tgatctccca gcaggatcgt gtcagcggta
    18421 aggtaaaaat cagcacaata ctgaccagag tcgccaacag ccagcccaga gtggtggcaa
    18481 atccgccaag cacaaagttc agaatgttca aaacggtacg cataaaacct cgctttactg
    18541 tggttttcag taatgggcgg caattgtaac gtttttttgg gctggagcac gttttctctg
    18601 acggttacac tgataagaaa taatttcgtg tggatctaca gagtcatgga actgaaagcg
    18661 acaacgcttg gaaaacgtct ggcacagcac ccttacgatc gggcggtgat cctcaatgcc
    18721 gggattaaag tctccggcga tcgccacgaa taccttattc ctttcaatca attactggcg
    18781 attcactgta agcgcggtct ggtatggggc gagctggaat ttgtactgcc ggacgaaaaa
    18841 gtggtgcgtc tgcacggcac cgaatggggc gagacgcagc gtttttacca tcatcttgat
    18901 gctcactggc ggcggtggag tggcgagatg agcgaaattg cgtctggtgt tttacgccag
    18961 caactggatt tgattgccac gcgcactggg gaaaataaat ggctgacgcg tgagcaaacc
    19021 tctggcgtgc agcaacaaat ccgccaggct ttgtcggcgt tgccgttgcc ggttaaccga
    19081 ctggaagaat tcgataactg ccgtgaggcg tggcgtaaat gtcaggcctg gttgaaagat
    19141 attgaaagcg ctcggttgca gcataaccag gcgtataccg aagccatgct taccgagtat
    19201 gcggattttt tccgccaggt cgagtcttca ccgctgaatc cggcgcaggc ccgggcagtc
    19261 gttaatggcg agcattctct gttagtgctg gcaggtgcag gaagcggaaa aacgtcggtg
    19321 ctggtggccc gtgcaggctg gttgctggcg cgtggtgaag cgtcccctga gcaaatttta
    19381 ttgctggcgt ttggtcgcaa agccgctgaa gagatggacg agcggattcg cgaacggcta
    19441 cataccgaag acattaccgc acgcacgttt catgcgctgg cgctgcatat tattcagcag
    19501 ggcagcaaaa aagttccgat agtcagcaaa ctggaaaatg ataccgctgc ccgtcatgaa
    19561 ctctttattg ctgagtggcg caagcaatgc agcgaaaaga aagcgcaggc gaagggctgg
    19621 cggcaatggc tgacggaaga aatgcagtgg tcagtgccag aaggtaactt ctgggatgat
    19681 gaaaaattac agcgtcgcct tgcctctcgc ctcgatcgct gggtaagtct gatgcggatg
    19741 cacggtggtg cacaggcaga aatgattgcc agtgcacccg aagagattcg cgatctgttc
    19801 agtaaacgta tcaagttgat ggccccgtta ttaaaagcct ggaaaggtgc gctgaaggca
    19861 gaaaacgctg tcgatttttc gggccttatt catcaggcga ttgtgattct ggagaaaggt
    19921 cgctttatca gcccgtggaa gcatattctg gttgatgaat ttcaggatat ctcgccgcag
    19981 cgggcagcgt tgttagcggc attacgcaag caaaacagtc agacgacgtt gttcgccgtt
    20041 ggtgatgact ggcaggcgat ttaccgattc agcggcgcgc aaatgtcgct caccaccgct
    20101 ttccatgaaa actttggtga aggcgatcgc tgtgatttag acacgactta ccgttttaac
    20161 agtcgtatcg gtgaggtggc aaaccggttt attcagcaga acccaggcca gttgaaaaag
    20221 ccgctaaaca gcttaaccaa tggagacgaa aaagccgtca cgttattgga tgagagtcaa
    20281 cttgacgctt tgctggataa gctctctggt tatgccaaac cggaagagcg cattcttatc
    20341 ctggcgcgtt accatcacat gaggcctgcc agcctggaaa aagcggcaac acgctggccg
    20401 aagttgcaaa tcgactttat gaccattcat gccagcaaag ggcaacaggc ggattacgtc
    20461 atcatcgttg gcttgcagga ggggagtgat ggttttccgg ctgcggcgcg ggagtcgatt
    20521 atggaagagg cgctactgcc accggttgag gatttcccgg acgctgaaga acggcggtta
    20581 atgtacgtgg cgctgacccg ggcacgccat cgggtatggg cactgtttaa caaagagaat
    20641 ccctctccct ttgtggaaat actgaaaaat ctggatgtgc cggtggcgag aaaaccgtaa
    20701 gaaacaggtg gcgtttgcca cctgtgcaat attacttcag acggtccgcg agataacgct
    20761 gataatcggg gatcagaata tcgaccgcgt cgttgaaatg cggcgactgg ataatgaagt
    20821 ctgccgttgc cacgttggtg gcgaccggaa tgttccatac cgtcgccaga cgcagcaagg
    20881 ctttcacgtc aggatcgtgc ggcacggcat ttagtggatc ccagaagaaa atcaatacat
    20941 caattttccc ttctgagatc aatgcgccaa cctgctggtc accccccatt gggccactca
    21001 acatcgcgtt gacgttcatg ccggttgcac gggaaatcaa gttaccggta gtgcctgttg
    21061 catacagtac gtgttgttcc agtaacggtt gatgccgttc cacccagctc atcagcattt
    21121 gtttgcagtg atcgtgtgcc accagcgcaa tatgtttccg cgcaggtaaa gtgcgagtcg
    21181 tcagttccat aatgtacatc cgtagttaac tttcctacag attactgtaa gcacttatcg
    21241 ctgcaagata aagaccgaaa aagcctgcgc acaggcacaa aaatctcagg aagatggttg
    21301 tttttccgcc cactgcagga aagtatttcg cgtttgtggg tcagccagtt taaaccaata
    21361 cttcagccgt tgttctgtga gcacctgaga ctgcggtgga atagcatcca gctcggaaac
    21421 acccgaaagc agcgtactgt catctgccat catcgtggca aattgcggta gtgaagcgcg
    21481 tttcgcggat ttgttatagc gttcaacctc gacctcataa tcaatcgttt ttgccgttga
    21541 ggtaatggcg agaatatcca gctttaccgg aatcggcgtc gcatcgccat ccaacaattc
    21601 aaggcgcggc gcggcatcaa aatggttagc ttcccgctca ttctccaggc gaggcaggcg
    21661 aaaattgacc tggttgatga gctgggtatt aaaactgacc accagcggtg gggagatata
    21721 cagccgttct tcactattgg aaagatgaat cgtcttctca acgcgaaaca ctaactgatg
    21781 cggtccatta tccagttcaa tgctgtccgc accgcgcagc agagagctgg aaaccttctt
    21841 gccgtcgagt accagaagat cgacatcggt tgaaagccgc agcgtggtgg caaaaacgga
    21901 taccggcaaa cataatgcaa tcaaggtggt cacgatgccg gttttcatag gtagctcctg
    21961 tcaaaagacc gcccgaataa taatgtgtca aaatttttct ttaatcatac ttactaacat
    22021 atagacatat ttcccgcttt tgctctcatt cattcgtatt agctgcatgg ttggcatgtc
    22081 ggctttgtcg tacactttgt taaacagtct ggaggaaata atgaaagaaa ccgatattgc
    22141 aggcatttta acgtccactc acaccattgc gctggtgggg gcaagcgaca aacccgatcg
    22201 tcccagctat cgcgtgatga aatatctact cgaccagggg tatcacgtca ttccggtttc
    22261 gccaaaagtt gccggcaaaa cgctgctggg ccagcagggg tatggcacgc tggcagacgt
    22321 tccggaaaag gtcgatatgg tcgatgtttt ccgtaattca gaagcggcgt ggggcgtggc
    22381 acaggaagct attgccattg gcgcgaaaac cctctggatg caactggggg tgattaatga
    22441 gcaagcggca gtactggcac gggatgccgg gttgaatgtg gtgatggatc gctgcccggc
    22501 tattgagatc cctcgcctgg gcctggccaa ataaaaaatc cccggaaggc aaaaaccttc
    22561 cggggattcg ttcagggatc agttacgcag acgcggggcc tggagttgtt tgcggatggt
    22621 ctgcgccagc tcatccatgg atggctgttc aggatgttca tcctgcaatt cgctacttag
    22681 ctgggcttca gccaggtagg tatgaaccgg taggccgtta tcgtcctcca tcaccacgtg
    22741 ataccacgga gcggcgcgaa gctcgtcatt caccgccagc tcatcaggcg acggttcaga
    22801 gagcgaataa accgggtcga tatccacgac cactccgaga taacctaaca gggaatggcg
    22861 gacctgctgg ccgataccga atttgctggc aatcatagtc acctcccggg aaatctttac
    22921 ctttgatata agggtaaaat tccacatttc aagttacatg acgcgacagg caaacccttt
    22981 cagatatagc ccttccggat aggtagcgat caccggatga tcggctgcct gacggaactg
    23041 ctctataaat tgtacatcac ggccggcatc aattgcggca tccgcgatga ttttctgaaa
    23101 taaatcgctg gtcatcagac cggaacagga gaaagtcagg agaataccgc cttcattcag
    23161 caactgaatc gccagcatgt tgatatcttt atagccacga cacgcgccca tcaactggct
    23221 tttattctca acaaacttcg gcgggtccat cacgataacg tcaaatattt caccgcgatc
    23281 gcgataagta cgcagcaatt taaagacatc atcacggaca aactcagcct tgctcagatc
    23341 cagtttgttc agctcaacgt tctgccgtgc aatatccagc gcttcctggg aggtatcaac
    23401 gctgacaacc tggctgcaac cgcccatcag tgccgatacg gcgaaaccac cggtatagga
    23461 gaaacagttc agcacacgtt tattttcaac gtagcggcgg gtagccaggc ggctatcacg
    23521 ctggtccagg tagtagcccg ttttgtgtcc gtgctgaata tccaccagca gcttcatgcc
    23581 gtgttcttca atcggcagca gggcaggcgg caactcgccg gtgacgaggc cctgggtcag
    23641 ctccatccct tcttttttac gtaccgcaac atcgctgcga tcgtaaatcg cacattccgg
    23701 gtacagcgtt tgcagggcac taactaatgc cgcgcgctga tattctgccc cagcactcag
    23761 cagttgcagc accagaaaat taccgaaacg atcgatagta atacccggca ggccatcaga
    23821 ttctccggcg attaaacgat agctgtcgag gccatctttt tgcgccagcc agtcacgcca
    23881 tttttgtgct tgttgcaaac ggcgggaaaa aaaagcaatg tcgatagact cagacgggtc
    23941 aaacgtccag acgcgcgccc ggatttgcga agctggtgaa taagcgccgc gtgctaacca
    24001 ttttccctga tgatcaacaa tatcgatggt ttcaccgagg ctggctttac cttccatgcg
    24061 ggcaacggcc ccggaaaaga cccacggatg gcgacgaagt aatgattttt cgcgcccttt
    24121 ggctaacact aaacgtacac tcataattta cttttctgtc gatgcaaaga aatgggcgtc
    24181 attgtccgga gttttccatg gatttgcaac gcgtccatta aggataagga gagcagcaat
    24241 gtcgaaagtc tgcataattg cctgggttta cgggcgagtt cagggcgtag gatttcgcta
    24301 caccacacag tacgaagcga aaagactggg tttaaccggg tacgccaaaa atcttgacga
    24361 tggcagcgtg gaagtggttg cctgcggtga agaagggcag gtggaaaaat taatgctgtg
    24421 gttaaagagt ggcggcccgc gttctgcgcg tgtagaacgg gtgctcagcg agccgcatca
    24481 tccctcgggg gaattaaccg attttcgtat ccgttaaata cattttaccg gttttggcag
    24541 gccagcaatt ttggtggctt gcttagccgg acctttcgga aacagtcggt ataaatagcg
    24601 gctgttacct ttttcttcgc caaatttatt cgccatcgct tttaccagca tacgaatcgc
    24661 cggagaagta ttgaattcca gatagaaatc acgcacaaaa cgcaccactt cccagtgttc
    24721 cggtgacagc gaaatccctt cgttctctgc aatcaccact gccagcggct cactccactg
    24781 gctgctttct ttgagatagc cttcggtatc cgtttctatc tctttacctt cgaagatcag
    24841 cataattatt actaccttaa tcagactggc ggcagtgtaa caaaaaacaa agccccgcat
    24901 aaagcgaggc tatgaaagtg ttagcgggtg agattaatcg cggctggcga agcccagaat
    24961 gctcagcagg ctgacgaaga tgttgtacag cgaaacatac aggctaaccg tggcacgaat
    25021 atagttcgtt tcaccgccat gaacgatgtt gctggtttca aacaaaatag cgccagagga
    25081 gatcaaaatg aagaccgcgc tgatcgccag atgcagagca ggcagctgca ggaagatatt
    25141 agcaaccata ccaatcagca ccaccacaat acccgccatc agcataccgc cgaggaacga
    25201 catatctttg cgggtggtca gcacatatgc agagcagcag aagaacacta acgccgttcc
    25261 gcccagtgcc atagcgatta cgtcacccat tccggcagac agataggtgt tcagaatagg
    25321 cccgaggata taacccagaa aaccggtaaa ggcgaatgcg gagataatcc cggtcggctt
    25381 attcgccgtt ttataggtca ggaacatcaa accatacata cccaccagcg tcagaatcag
    25441 acccggagat ggcagcatca gcaccgtact ggcagttgcg gtaattgccg aaaaggccag
    25501 cgtcaggctc agcagaaaat aggtattacg cagcacctta tgggtgctaa gcagtgatgt
    25561 acggtcatgt gaagaactaa caatacgatc cattgagtca ctctctatga cagatgtaat
    25621 taattaagca gcataatgat aatgcgtaag ggcacccaga agttttaccc atctttacgc
    25681 atttgatctg gaacaggttt aacagcggat tatcaggtca ttaagcaaat ataacgccct
    25741 gagaatttcg acaggcaaaa gaaaaagggg ttagcattta gctaacccct tatcttattt
    25801 ggcggaagcg cagagattcg aactctggaa ccctttcggg tcgccggttt tcaagaccga
    25861 agaaaaacaa ataaaaatca gtgcaatacg tgaaattgct gaaaagttaa tcaaatttaa
    25921 caccaagaaa aatcaaggta ttaggaagtt gctcatagct agtataccaa gtctttttgc
    25981 cagaaatttt tctggattgt ctacttgcta accacgagcg gcaaatccag tgttggtgtg
    26041 atttttgttt tacgatcgta aatcaacacc tgattttctg ttttatgccc gctgaaaatt
    26101 tgtttgtcgc gactgctgcc ttcgtaatct gagatcccct tggcttttat gtcgtgaaaa
    26161 ttgcacccga acggaacgcc aactttttgc tcggctgcgc gtttagcctg attccaccag
    26221 ttattcagcg ttttagctat gactttcccg cctttggttg tattgatcac atattcgcat
    26281 gtacaggaag atacatttcg ggctaactgg atcgctgtac gtaatcgcgg tgaccattcc
    26341 ttgatttgtt tggtgccggt tttgttttgc tcaatgtaga tccctttatc cataatatcc
    26401 tgccatttca actcaagcac atcaccgagt cttgccgcac agagatagga aatctccatt
    26461 gcaatgcgta actgtggaat tgcttccgca tatatcgccg catactgttc atcggtgatg
    26521 taaacagtgc gggctttaag agagaatttt ctgactcctt tgcatgggtt attcttcaca
    26581 tacccacgct catatcccca cccgtatact cgactcaggc ttgccagttc atgatttgcc
    26641 tgggtcttgc tctcaagccc tcgtttatcc atgaaaattc ttacctgctc agttttgaca
    26701 ttatcagcaa gcacttttcc gaataccatc agcaacgcct tctgatgttg tcgataatct
    26761 ttttgggttc ggggggccag ttctgtaaat gcaggggagt ccataaacat gtgccataat
    26821 ttagctacgg tcattatgtt gtggagtttt gctttttcca gttcataatt ttgccagact
    26881 ttagctacgc tggttttccg cactcttcct agccctatac ttcttgtact tccttcgggt
    26941 ttccacacgt aactgtaacc attcgatcta acccgcggtg gcagtacatt atctttttta
    27001 ttttttcttg gtcttcccat tgttcagcgc ctcaaaatcg ggttcagcag aaaccagttc
    27061 agatgttttt ggcatcgttg tcagaccgtg tggaatatcc ctgcggagaa ctatgggttc
    27121 gtttttaggg ccgattacaa atgggatgcc gtgcagtctt aactggtgtt gctgttttgt
    27181 gtatcgctcg tatttcgtga tctctttaat ctctgctgga gatagagtta attcgtacat
    27241 gtggtcacgt gcctttacag catgaccgcc gccactatag ctggtgggcg gcgatatggg
    27301 ttgaacatca aaaatcagcc tgactcggga gcagtttttg ccagatggct gaaacgtatt
    27361 tcgcctggta acgtgcatcg ttcagagcgt tatggcgttc accttcgaat ggaatagtcg
    27421 ttcttgcgtc gaaatccatc accagtccca gagcaaccat cgttcttaca tcgcgatcat
    27481 tggtgtaacg ccacgggcag gggatcccct gccgttcata tgaacggcgt aaaatcacgt
    27541 tgtcgaaagt tgcaccgtta ccccagacct gaacaaaaaa ttcaccggag ttttcgtcga
    27601 taaattcccg taattgcagc agtgcatcat ctaacgggat ttcatcggta agaatggcgg
    27661 attgcgcttc gcgtgactgt ttcagccacc acttaatggt gtcccgatca atgactccgc
    27721 cagcagtttc cagatcgata gttttgctga attctggccc catctctccg gtttgcggat
    27781 cgaaaaatat tgcacctatt gagataatcg gggcatcagg attttttccc atggtttcaa
    27841 ggtcgatcat cagatgaatc cccgctctgc tggtggatgt gatattatga tgaccgttcg
    27901 ccttaattaa gggatctgac gcctcgccag tttcactatc gctggcatga tgctgattgc
    27961 cgccagtgtt ctccttgtgc tgatgcgcag tgccttccat ttcctccgga tcattttcct
    28021 gaacttcagg ctgattctct tcatcgaata tttcctggta tgttgcgtca cttatcaccg
    28081 caccacaatc agggcagcta ccgccgccgg tctgaccgca ggcggtgcag gctttttccg
    28141 gctcctgttt cgtttctggc tcgttttgtt gcgcatttgg gctgttttgt tccgttttct
    28201 ggtcgttctg ttccgattct tgctggttct ggttcacaga atcgcaggtt tcaatcccct
    28261 tcacccattt cggatcattc ggatcgctaa tcccttcaac aaattcacca cgtgatgcag
    28321 caagcaattt atcggcgtca ggctggctga tattggctgc ctgcataatt ttgtttactt
    28381 cgtcagcggt aacttttacc ggctccggtt gtacggaatc ttcagcggta tccacatttt
    28441 gcggtaagtc cgtgtatgtt ccgtttttgc gggcaa
//
LOCUS       DAZGOB010000055        28392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGOB010000055 DAZGOB010000000
VERSION     DAZGOB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.055"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..729
                     /gene="ybjI"
                     /locus_tag="ACHLIF_002545"
     CDS             <1..729
                     /gene="ybjI"
                     /locus_tag="ACHLIF_002545"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO9606541.1"
                     /translation="YQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVV
                     SEGKDVFNGELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYH
                     RLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAIGDIMVPVHTGNGSIDLIIPG
                     VHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVAAAKYRA
                     GSNNREGVLDVIDKVLKHEAPFDQ"
     gene            815..1099
                     /gene="ybjH"
                     /locus_tag="ACHLIF_002546"
     CDS             815..1099
                     /gene="ybjH"
                     /locus_tag="ACHLIF_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO9606542.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(1140..2372)
                     /gene="mdfA"
                     /locus_tag="ACHLIF_002547"
     CDS             complement(1140..2372)
                     /gene="mdfA"
                     /locus_tag="ACHLIF_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO9606543.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            2657..3253
                     /gene="ybjG"
                     /locus_tag="ACHLIF_002548"
     CDS             2657..3253
                     /gene="ybjG"
                     /locus_tag="ACHLIF_002548"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO9606544.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            3311..4069
                     /gene="deoR"
                     /locus_tag="ACHLIF_002549"
     CDS             3311..4069
                     /gene="deoR"
                     /locus_tag="ACHLIF_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO9606545.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(4116..5318)
                     /gene="dacC"
                     /locus_tag="ACHLIF_002550"
     CDS             complement(4116..5318)
                     /gene="dacC"
                     /locus_tag="ACHLIF_002550"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO9606546.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            5565..6191
                     /gene="gstB"
                     /locus_tag="ACHLIF_002551"
     CDS             5565..6191
                     /gene="gstB"
                     /locus_tag="ACHLIF_002551"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO9606547.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(6188..7303)
                     /gene="yliI"
                     /locus_tag="ACHLIF_002552"
     CDS             complement(6188..7303)
                     /gene="yliI"
                     /locus_tag="ACHLIF_002552"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO9606548.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(7414..7797)
                     /gene="bssR"
                     /locus_tag="ACHLIF_002553"
     CDS             complement(7414..7797)
                     /gene="bssR"
                     /locus_tag="ACHLIF_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO9606549.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            8010..9335
                     /gene="rimO"
                     /locus_tag="ACHLIF_002554"
     CDS             8010..9335
                     /gene="rimO"
                     /locus_tag="ACHLIF_002554"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO9606550.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(9382..10710)
                     /gene="dgcI"
                     /locus_tag="ACHLIF_002555"
     CDS             complement(9382..10710)
                     /gene="dgcI"
                     /locus_tag="ACHLIF_002555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9606551.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(10718..13066)
                     /gene="pdeI"
                     /locus_tag="ACHLIF_002556"
     CDS             complement(10718..13066)
                     /gene="pdeI"
                     /locus_tag="ACHLIF_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9606552.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVLERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(13244..14155)
                     /gene="gsiD"
                     /locus_tag="ACHLIF_002557"
     CDS             complement(13244..14155)
                     /gene="gsiD"
                     /locus_tag="ACHLIF_002557"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO9606553.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(14158..15078)
                     /gene="gsiC"
                     /locus_tag="ACHLIF_002558"
     CDS             complement(14158..15078)
                     /gene="gsiC"
                     /locus_tag="ACHLIF_002558"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO9606554.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(15096..16634)
                     /gene="gsiB"
                     /locus_tag="ACHLIF_002559"
     CDS             complement(15096..16634)
                     /gene="gsiB"
                     /locus_tag="ACHLIF_002559"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO9606555.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(16654..18525)
                     /gene="gsiA"
                     /locus_tag="ACHLIF_002560"
     CDS             complement(16654..18525)
                     /gene="gsiA"
                     /locus_tag="ACHLIF_002560"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO9606556.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(18512..19477)
                     /gene="iaaA"
                     /locus_tag="ACHLIF_002561"
     CDS             complement(18512..19477)
                     /gene="iaaA"
                     /locus_tag="ACHLIF_002561"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO9606557.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            19681..20916
                     /gene="moeA"
                     /locus_tag="ACHLIF_002562"
     CDS             19681..20916
                     /gene="moeA"
                     /locus_tag="ACHLIF_002562"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO9606558.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            20916..21665
                     /gene="moeB"
                     /locus_tag="ACHLIF_002563"
     CDS             20916..21665
                     /gene="moeB"
                     /locus_tag="ACHLIF_002563"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO9606559.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(21741..22403)
                     /gene="fsa"
                     /locus_tag="ACHLIF_002564"
     CDS             complement(21741..22403)
                     /gene="fsa"
                     /locus_tag="ACHLIF_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO9606560.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGNKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            22534..23433
                     /gene="ybiY"
                     /locus_tag="ACHLIF_002565"
     CDS             22534..23433
                     /gene="ybiY"
                     /locus_tag="ACHLIF_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO9606561.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            23439..25871
                     /gene="ybiW"
                     /locus_tag="ACHLIF_002566"
     CDS             23439..25871
                     /gene="ybiW"
                     /locus_tag="ACHLIF_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO9606562.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            26017..26832
                     /gene="ybiV"
                     /locus_tag="ACHLIF_002567"
     CDS             26017..26832
                     /gene="ybiV"
                     /locus_tag="ACHLIF_002567"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO9606563.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            26984..28249
                     /gene="ybiU"
                     /locus_tag="ACHLIF_002568"
     CDS             26984..28249
                     /gene="ybiU"
                     /locus_tag="ACHLIF_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO9606564.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVISPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         7154 a         7520 c         6904 g         6814 t
ORIGIN      
        1 tatcagcaaa tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat
       61 cagttgatct ctttcttccc tgaaattgcg aatgaaattg cctttgtggc tgaaaacggc
      121 ggctgggtag tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgcgttt
      181 gctactgtcg tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa
      241 aatagcgcct atacactcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat
      301 tatcaccgtc tggaatacgt cgataacttt gacaacttag aagatatttt ctttaagttt
      361 ggcctgaatc tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc
      421 ggcgatatta tggtgccagt ccacaccggc aacggcagca tcgatctgat tatccccggc
      481 gtacataaag ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa
      541 gtggtggtct ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt
      601 tttgcaatgg aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg cgcaggctcc
      661 aataaccgtg aaggcgtact ggatgtgatc gataaagttc ttaaacacga agcgccattt
      721 gaccaataaa aacggcagca cgcccggcaa cacaatgcct gaatgaaggc tgcataattc
      781 gcggaaatta acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt
      841 gggcgcgctt ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt
      901 gccgagtgga tacaaagtgg ttgtggttcc tgattcagtc agcgttccac aagcggtcag
      961 cgtcgcgact gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc
     1021 gcatccttat gttcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat
     1081 tgacgaccat caccattaat tcgagaatgc ctgatcgcac aaagcaatca ggcatttttt
     1141 tacccttcgt gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc
     1201 cacaaaattc cgttgacaag gttgaagaga ttaaacagtc cgttgccccc gttcagccag
     1261 gcatgtttgc tgatttcaat accgacggta aagatcagca tttgcagcat tcccatcgcc
     1321 gcagaaaccg tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca
     1381 ttcgccagac caatgccgaa agcataaata cttaacccgg cggtcatcca taaatacgcg
     1441 tgcgatgaga taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg
     1501 cccataatta tcagcgaacg tacggtgcgg cgcgaggtca aacgcgctaa caacaagtta
     1561 cccgcaatta acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac
     1621 tgctcgccgg taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta
     1681 acgaatccca gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag
     1741 tcacgaccga gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcatg
     1801 gcttgttgca gaccgaaaaa ggagatcgct gccaatgcgg caaacaagac aaacatccct
     1861 tcccagggca gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc
     1921 agcgccacgt tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaggattcc
     1981 tgaattgcgg cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc
     2041 aacagagtga attgttcaat gttttgcgcc agtaatattg ccagacaggt gatgataaac
     2101 cacaccaccc ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg
     2161 agccattgta aaaacatccc gcccgccaga tatgcggtca ttgaagtagg aacccaatca
     2221 atgcccgcct gatattgttc caccacggcc aacataccgg gttgaatcat atcgttgccg
     2281 atataggttg aaaattcgta aagcaccaga cagagaggga aaagtaacgc ctgacgtcca
     2341 agcctggcac cggaagctaa tttattttgc atgcaatttc ttcgccaata ataatcgcgc
     2401 agagtttaat aaaagcacag ctaacgagaa agcgaatttt gtagctgaaa ccacggttaa
     2461 gcacattctt acattattgc gagtatagct acgctttctt taagttttat ttaacctatg
     2521 cccgttacaa ttacccaccg taaacaggcc gcttgaggga aataagacga tgccgcttta
     2581 cccagtttaa cctgcacttt attctcaacg acttgcctgt attggctccc ttttaatcac
     2641 tttgcgtcgg gaagttatgc tggaaaattt gaatctctct ctattctctc ttattaacgc
     2701 gacgccagac tcggctccgt ggatgatctc gttggcgatt tttattgcta aagatttgat
     2761 taccgtggta ccgctgctgg ccgcggtgct ttggttgtgg gggcttacag cacaacggca
     2821 actggtgata aaaatcgcta tcgcgctggc ggtcagcctg tttgtgtcct ggacgatggg
     2881 acatcttttt ccgcacgacc gaccctttgt cgaaaatatc ggctataact tcctgcatca
     2941 tgcggcggat gactcattcc caagcgacca cggtacggtg attttcacct ttgcactggc
     3001 atttttatgc tggcatcgcc tgtggtccgg ctcactttta atggtgctgg ccgtcgtcat
     3061 tgcctggtcg cgcgtttatc ttggcgtgca ctggccgctg gatatgctcg gtggattgct
     3121 ggctggtatg attggctgcc ttagcgctca gattatctgg caagcgatgg ggcataaact
     3181 ctatcaacgt ctgcaatcgt ggtatcgctt ctgttttgca ttaccgatcc gcaaaggctg
     3241 ggtgcgtgac tgatctctgc taaaaagtgt agtattgagc ggctcgcttc aataactatt
     3301 cagagggatt atggaaacac gtcgcgaaga gcgcatcggg cagctgctgc aagaattaaa
     3361 acgcagcgat aagttacatc ttaaagacgc cgccgccctg cttggggttt cggagatgac
     3421 gattcgtcgc gatctgaaca accacagtgc gcccgtcgtt ttgctcggcg gctatattgt
     3481 tctggaaccg cgcagtgcca gccattacct gttaagcgat caaaaatccc gcctggtgga
     3541 agaaaaacgc cgggcggcaa aaatggctgc gacactggta gaacccgatc agactctctt
     3601 ttttgactgt ggcaccacca cgccgtggat tattgaagcg attgataatg aaatcccttt
     3661 taccgccgtt tgttattcgc taaatacctt tctggcgctg aaagagaaac cccattgccg
     3721 cgcatttctg tgcggtggtg aatttcacgc cagcaacgcc attttcaaac ccatcgattt
     3781 tcagcaaacg ttgaataatt tttgcccgga tatcgctttt tattccgcgg cgggcgtgca
     3841 tgtcagtaaa ggcgctacct gttttaatct tgaagagttg ccggtaaaac actgggccat
     3901 gtcgatggcg caaaagcatg tgctggttgt cgaccacagt aaatttggca aggtgcgtcc
     3961 ggcgcgcatg ggtgacctga aacgctttga tattgtggtg agtgattgtt gcccggaaga
     4021 tgagtatgtg aagtacgcgc agacgcaccg tattaagttg atgtattaat gacgtataac
     4081 cggatgacgt ttcgcgccat ccggttatca gaagattaag agaaccagct gccgaaccac
     4141 tgatggaatt tcatcatcac gaaatcccac atccgaccaa agaatccgcc ctcttccaca
     4201 ttttccatca cgatcagcgg acgctgctca atggatttac cgttaagctg gaaatcaatg
     4261 gtcccgacaa cctgaccttt tttcagcggt gcggtaagct gcggttccgt taacgtgtag
     4321 ctcgccttca ggtttttcag ctgcccacgc ggtatggtca ctgagcccgc ttcgcctgcc
     4381 ccaagattca cttcgctctt atcaccaaac cagacgcgct gagtcacaaa ggtggcatca
     4441 ggtttaattg gcgtcacggt ttcaaagaag cggaaacccc aggtcaataa tttctcagac
     4501 tcattaaaac ggatacggtc agttttcgcc cccagcacta cggagattaa acgcatatcg
     4561 ccctgggtag ccgaagcaac cagattatat cccgcgcctg ccgtggttcc tgtcttcatg
     4621 ccatcaacat tcagattgct gctccacagc agacggttac ggttaggctg acgaattttg
     4681 ttgaaggtga actctttctc tttatgaatg gcgtactctt ccggcacatc gtggatcaat
     4741 gctttaccca gcaatgccat atcgcgcgcg gtactgaact gccccggcgc atccagaccg
     4801 tgcaccgtct ggaaggtagt gttggtcaga cccagttttt tggcataacc attcatcaaa
     4861 ccaatgaatg actcctggct cccggcaacg taatcggcca gcgcaataca ggcgtcatta
     4921 ccggactgga taatcacacc tttgttcaag tctgccaccg aaacctgatc gcccggtttg
     4981 aggaacatta ccgatgaacc acgcagtgcc ggatttcccg tcgcccaggc atctttaccg
     5041 accgtcacca tatcggtgag tttaatctta tcggccttaa gcgcctgccc aaccacatag
     5101 ctggtcatga ttttagtcag gctcgcggga tccagtttct catccgcgtt gccttctgcc
     5161 agcactttac cgctggcgta atccattaaa atccatgcac gcgcatccac gctcggcgct
     5221 tcaacggttt gttccgccgc gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc
     5281 gctgcaagac cacgaaggag agaggagtat tgcgtcataa atgccacccg agtatccatt
     5341 caaaaaaaat acgttatatc gccgtatgat aagttcggtg attaataacg cacacacaaa
     5401 cttaaagaaa ccgtaagttg gtaaagtttt taaagtttat gcaatatcct gccgcttcgc
     5461 tgcttctggt gtcatttttt tgccagctgt tgcttaaaag ttaactttac ttcaccatcg
     5521 gagtcctgtt cagcctgtgg ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg
     5581 gtcggaataa ttcaaccaac gtaaaaaaag tattgctgac gctcgaagaa ctggaactac
     5641 cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct gattttctgg
     5701 cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat ctcattcttt
     5761 gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa cgtctgtgga
     5821 tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca aaccagacgc
     5881 tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg gaagagcgcg
     5941 atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc ctgctcgatg
     6001 cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc gatatcgcta
     6061 tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg cgtccaaatc
     6121 tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg gtgatgattc
     6181 ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg tcggtgagaa
     6241 cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct ctgtccgtta
     6301 aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg acatctttat
     6361 ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg ctgttataga
     6421 aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg
     6481 cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt ccccaggttg
     6541 ccagcggcca gccgtagttt ttgccttttt gcgggatatt aatttcatca ccaccgcgcg
     6601 ggccatgttc attcagccac agtgcattac tccacggatt cattgccatt ccttgcggat
     6661 tacgaatgcc ataagaccag atctcggcgc gcacaccgga ttcctttata aaaggattat
     6721 catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat ttatccagat
     6781 cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa cctttaccgt
     6841 cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc tggcggaaga
     6901 cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa cccacagcag
     6961 ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg cgagactgag
     7021 caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc caaacgtccg
     7081 gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc aactcgccgc
     7141 cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc cagggatggt
     7201 cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag agagcggaag
     7261 aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct ccggattgac
     7321 tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa aaacttaaac
     7381 atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca tggcgtgctt
     7441 ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct tatcggcgtt
     7501 gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg cggctgtcgc
     7561 taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat gaagattcaa
     7621 ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc gtagatgatt
     7681 gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg cagcgaggtc
     7741 aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa cgaacatact
     7801 tcgatcctcc tctttccagc cttgttgcaa ttaaatagta tggctatttt ttgagcaaaa
     7861 tgctgtccag gatcaattct tcacgccgaa ttaacaaaat ttacatttcc ttcgcctccg
     7921 ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc ctgccctgac
     7981 ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag ccgaaaatcg
     8041 gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt ctcaccgaac
     8101 tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg gtgatcgtca
     8161 acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt ggtgaagcgt
     8221 tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc
     8281 gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag caggttctgg
     8341 agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc ctggtgccag
     8401 aacaaggtgt gaaactgacg ccgcgtcatt atgcctatct gaaaatttcg gaaggctgta
     8461 atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg agccgtccga
     8521 ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa gagattctgg
     8581 tgatctcgca ggatacttct gcctatggcg ttgatgttaa acatcgtact ggcttccaca
     8641 acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg aaactgggga
     8701 tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc atcccactga
     8761 tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc agcccgcgca
     8821 ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc atcaaacagt
     8881 ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc ttccctggcg
     8941 agacagaaga agatttccag atgctactcg acttcctgaa agaagcgcgt ctggatcgcg
     9001 ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg cctgaccagg
     9061 ttccggaaga agttaaagaa gaacgctgga accgtttcat gcagctgcag cagcagattt
     9121 ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg
     9181 acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt
     9241 atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa gtcgagcacg
     9301 ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg caaaatcatt
     9361 tgtccggcct gcgaataatg attactgcca gctaatctga gccctgccgt ttttcttact
     9421 cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac attcatttga
     9481 atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat gaacgatttc
     9541 ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg caaattcgtc
     9601 accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa ggattttcac
     9661 cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa tgtttttgaa
     9721 tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt ttaaatcttc
     9781 gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg agttatacat
     9841 actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca gtacgcgttc
     9901 aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca ctctgcgtac
     9961 cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga aaataatcaa
    10021 tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc cggtcggcaa
    10081 ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag cataatattc
    10141 atcaccggtc accggtttac cgttcttaaa tagagagaac atcaggttct cgctgtccat
    10201 cgccagcgat tttgacagta aagaaaaacg ctccagacca agcaaaaata tcagactacg
    10261 attgtgagtt accggataga ggtaatagac gaaggtttta ccgtcaaaag agataaaccc
    10321 tgtatttttg acatgtcgat acttcacaac ctgcataata tctgcacaat aatttttctc
    10381 gaactccttt acctgaatac attcgtcact ggaaatcaga taaggaccga catcgtacaa
    10441 ataagagggg gaaaccgagt cggcgtaagc tgtgcgcaaa ccaaccaaca gctgttcctc
    10501 cgtgggattt agcgtgttga taagatcaat tgccacgcta cgccgatatt cagcaatatt
    10561 tgccaccgca aaagcagtat caataacgga ctgtttcagg ctatacaccc gttcctttac
    10621 catttgagtg tagatcccgc aggcaactgc tgaaatcatg ataaaaatca gcagactgac
    10681 tgtaagtacg aacttattga ttctggacat acgtaaacta ctcttttact aattttccac
    10741 ttttatccca ggcggagaat ggcatgggtt tctggtacag atacccctga gcctgaacac
    10801 atcctaattt aattaattcg cgcgcaacgt cttcgctctc tacgccttcc gcaatcaccg
    10861 ggatccggtt atagcgtgcc agatcgaaaa taatgcgcac aatttttccc gcagtaccga
    10921 aaatatcgcc ggtaaattgc gagtccacct ttatgctgtc tggattaatt tcataaaaac
    10981 gtttcaggtt tgacaaaccg gtgccaaagt catcaatcga tatccgtacc ccttgtttac
    11041 gcagttgttc aatattgaga taaaaatgtt cattaattcg ctcgatcacg gtttcagtga
    11101 tctcaacaca gaggctgttc gccttcaggc cattttcttt catgatggtg gtaaaacgct
    11161 catgaaagtg cggttcgttt aactgccagg gcgaaacatt aatatgcagt aagaaatcat
    11221 ccttcagcga cgcacgctga ctaaaatgtc tgaattcacc cattgccgtt ttaataatct
    11281 gataacccag ctcattgata aacccgatat cttcagcaat ggtaatgaac ttcagcggtg
    11341 aaatgatccc ccgctccgca gaaacccaac ggcacaacgc ctccgcgccc agaatggcgc
    11401 gggtgttaat atcaacgatc ggttgataga cgaggaatag ttcgccaatt tccattgcat
    11461 ggcgaatatt acgcgcaatc cagatattct cttctatttc gttgagtttc gcgtcatcac
    11521 agaaaataaa ctcggagaac atatggtcac ggatactgga aatcgccagt ttcagttttt
    11581 ctgcgtaact ccaggcgttt tccccctgca aaggttcaca aatggcagac ttatggatgc
    11641 gcagattgcg gtagccaaag ctgctgtcaa aatcgtttac catcgctatt ttacgggtaa
    11701 atgtctgtac attttcccga cacagaagta aatagagatc ggcattgaat gtcacgatat
    11761 cgcagcattg cgcatatttc tcggcaattc gctgacacaa tttcctgacg attttattgc
    11821 gttcatcaac accaaacacc gcttccacgg cttccagacc acacagtttg atgatcccca
    11881 cagccagata ctgattattg ttggactcaa taatcgccat atttttaata aaaccgcttt
    11941 cctgatcgta agagatcagt gagagcaatt tatttgaccg ttcaatttcg gcatctttca
    12001 tttccgcgat cgaatttttg atgctgataa tctctttcgc atcgccttcg cgaattttca
    12061 gagcctcgat ctgatccgtt gagtcgggca aaaacgccag cgtattaacc agctcggtca
    12121 tttcagaaaa ttgccgacgg aacacacgag tggtcatgaa atataccacc agcaacacga
    12181 taataatgaa cgcaaacggt agataaaaaa gtttatctga tgcgttcaat aaattaccgc
    12241 caggataata aaaatagatt tcgaacaatg cattatttgg cactgctgat gtctttgcaa
    12301 tatactccgt atcgacagaa tcaataatat tataattacc ttcgctatta taaataatca
    12361 gtttttcacg agcgagttga ttcttggcaa tgatctcatg accttgtttc aaaaatagaa
    12421 cttcatcatt agcgctgatt tgatcaagat actcgttcag ataccgccag tttaaatcga
    12481 catgaatttt atctaccgct aacaaatcgt gatctttgcc gttgatacgt agcgtaaaag
    12541 agatacattt tttacccgga cgatcaaagc aattgtatgc cggattccag ctgttgttag
    12601 agtcatctga agccaccgac cacggtcgtt tcgatgggtt aaaatcggca aaaataaccc
    12661 gccctgactc gtcgacaagc ggtccattta cttgttcctg atgataaaca acgattttat
    12721 cattcgccct ccgtgaaaag agataatatt tattatcatc aagtaccagt cccaccgagt
    12781 ctaaaaatgg catcatttcc attttcagaa tcacggcgcg ttgaatctcc tctgtatttt
    12841 ttaataaata cagtgagtcc tttaatgtca gggccgaacg ttcaatatct ttaaagaaat
    12901 agccaacgtt tttctgaata agttcaaagc ggctatcggc acgttttacc gcgcgttcca
    12961 ataccagttg atagttaatg atgaaaaaaa gaccaataaa tgacagtgct gccaataaaa
    13021 ataaaattat cagccttatc tttatctttt cgtataaact cagcatgtta atacatttaa
    13081 caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc ggctttttaa
    13141 ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta gggcaataat
    13201 ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct ttaattttcg
    13261 gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa
    13321 tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct cgtgcctcat
    13381 tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc
    13441 tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg atagacgaga
    13501 cggtcccagg caagatatga cgcaataaaa tggtcatatc gctggcacca atactgcgtg
    13561 ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc agtcgggcaa
    13621 acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg cttcccaaca
    13681 ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc
    13741 gcatgatcaa ccgatcccac cagccttcat aatatccagc caacaagccc agcaacgtcc
    13801 cgatcgccgc accgataaac acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa
    13861 ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag
    13921 aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag ggagcgatcc
    13981 agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg gtcatcgcca
    14041 tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgcacc tggtcaggtt
    14101 tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt cgcatcctta
    14161 cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta agttgataag
    14221 aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat cgcgcatttc
    14281 tacggagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct ccaccacgat
    14341 agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg ggatcatcgc
    14401 gttacgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg ccctcgcggt
    14461 acgcatataa tcttcgctta aaacatcgac aaacgacgcg cgggtaaagc gcgccatcac
    14521 ggctgccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc
    14581 tcccacggta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa gcatccccag
    14641 cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc gatccggcca
    14701 acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca ccgcccagac
    14761 catactggtt atggtcagcc acagcgttgg cataaagcgg ctggcaatct catcggcaac
    14821 cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc tgatatagtg
    14881 ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca gttctataac
    14941 ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca gcatatgcac
    15001 aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca gtaagcgttt
    15061 gataacgtaa ttaagcattc cactccctgc gttgcttatt gcaaatccgc gtcttcaaag
    15121 ctgaagccgg tgtctggcat gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc
    15181 agtttttcta ccaccagcgg gatccacggc gactcttgcc agatgatatc ctgcgccgcc
    15241 ttatacaagc gggtcttttc cgccggatca ttagttttca gtgcctgagc caggaagtcg
    15301 tccacctgtt tattgctgta aaatgcggta ttaaacagcg tcggtggcca gttttgcgag
    15361 gcgaacagtg gcgatagcgc ccagtccgct tcgccggttg aagccgacca gccagtgtag
    15421 aacatccgca cgccgctctc tttttgccct ttaccttcaa cttctgccgc ccgctgtccg
    15481 gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat
    15541 tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt actgaaaccg
    15601 ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta aggccacggt
    15661 ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg cgttgcatag
    15721 cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag cgcctcacgg
    15781 accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg ctgcataatt
    15841 gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc ctgctcgtag
    15901 ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta
    15961 tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg ccagtaaccc
    16021 gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc atacggtccg
    16081 gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgc cggtgaaatc
    16141 atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg tttgagggta
    16201 atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata caggttatag
    16261 cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac cgccactgcg
    16321 ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacgat gtaagtaatg
    16381 ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat ctctttatcc
    16441 agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt gtcatttgcg
    16501 tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc tttggcagcg
    16561 aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc actacggtgt
    16621 acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg cgtattctga
    16681 ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg ctgccacctc
    16741 ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac gctgcggtcg
    16801 ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat aaggatgctg
    16861 cgggttttcg aagaccgcgc gccgtggacc aatttcaaca atttgcccga gatacatcac
    16921 cgccacacga tgactaatcc gctctaccac ggccatatcg tgggagataa acagatacgc
    16981 aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc gaatagaaac
    17041 atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg ccaacgcgcg
    17101 agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg
    17161 ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg cgtctttacc
    17221 tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg
    17281 gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca atgcctgaag
    17341 tttgccgggt gacaaggtat cgattctctg accgttaaag ataatttcgc cgccctgcga
    17401 ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag actcgcccac
    17461 cagcgatagc gtttcgccag gccagagatc aaaactaact ttctcaacgg catgcacttc
    17521 ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa gattacgcac
    17581 tcgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atgggggggg cctgtttcgc
    17641 tggatgttca agcgatatca acgggaaacg tcggggataa tctaaccctt tcatcgcacc
    17701 aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg catgaaaaat
    17761 ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta cccgatcggc
    17821 aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg acatctcttt
    17881 ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca gcgcggtggt
    17941 tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg
    18001 ctggcgcatc ccgccagaga gttgatgcgg ataacgtgaa agaatggttt gtgcctcagg
    18061 aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt
    18121 ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaataccg ggttcagcga
    18181 tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgaacatggc gcatttgtgc
    18241 agcgctctgc tcgctaagtt caatcacttc gcgactgcgc cgctgcaaca gcattttatc
    18301 gcactgtact aaaccgcccg cctgttccaa caggcgcatc aatgccaacg cagtcactga
    18361 cttaccggag ccggattcgc caacaattgc cagcgtctca ccgcgttgca gactaaaaga
    18421 gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat tcagattttc
    18481 aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg tccccttttt
    18541 cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt
    18601 cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag ccaccgctac
    18661 cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg agacttaatc
    18721 cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg aagacttcgc
    18781 ccgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg gcacccacta
    18841 agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg gacgtggctg
    18901 ccgccaaatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt ttctgttttt
    18961 catccagtgg cgcaccgcta tggtcgagga cggttgcccc ttccttgcgc gctgccagta
    19021 gttgttcata acgcaaaggc gtggagaaaa tctccggcga gacgcgctcc atgccacgag
    19081 caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt tgctccatca
    19141 ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc gcaccggctt
    19201 tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga
    19261 cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc gcttccgtca
    19321 ccacatccag cgcactttcg cccgcttcca acattttctg cccggtttca acaatggcag
    19381 acaacgcctc aatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac
    19441 ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat taagggtggc
    19501 tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat ttttgaatat
    19561 agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg tggcatacag
    19621 tctgtggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag gagtgttttc
    19681 atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc
    19741 gtcaccccac tgaccgccca ggaaacgctg ccactggtac agtgttttgg tcgtattctg
    19801 gcgagcgatg tcgtttcgcc gcttgatgtt ccggggtttg ataactccgc aatggacggc
    19861 tacgcggtgc gtttagccga tattgcctcc aggcaaccgc tgcccgttgc cggtaaatcc
    19921 tttgccggtc agccgtatca tggtgaatgg cctgcgggca cctgcattcg tattatgacc
    19981 ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg
    20041 gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt
    20101 gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac taccgcagag
    20161 ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa agtgcgtgta
    20221 gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg cgacggccaa
    20281 atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg ttgcgaggta
    20341 atcaacttag ggattatccg cgacgatccc catgccctgc gcgccgcatt tattgaagcc
    20401 gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga agcggattac
    20461 accaaaacga ttcttgaaga gctgggggag atcgccttct ggaagctggc gattaaacca
    20521 ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct gccgggcaac
    20581 ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc aaaactaagc
    20641 ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc ccgcctgaaa
    20701 aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc cgacggtgaa
    20761 cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt tagtctcggc
    20821 aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg aagtgggcga atgggtggaa
    20881 gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatgac ggaactcagc gatcaggaga
    20941 tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt caggaggcgc
    21001 taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca gcttcgcagt
    21061 atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgactcg gtttcgctct
    21121 cgaatctgca acgccagacg ctgcacagtg atgctacggt cgggcaaccg aaggtagaat
    21181 ccgcccgtga cgccatgacg cggatcaacc cacatatcgc gattacgcca gtcaatgcac
    21241 tgcttgatga cgctgaactt gctgcgatga ttgcaaaaca cgatctggta ctcgactgta
    21301 cggataacgt tgcggtgcgt aatcaactga acgctggctg ttttgccgcg aaggtgccgc
    21361 tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact tatcaggacg
    21421 gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcatta acctgcgtag
    21481 aagcaggcgt gatggcaccg ttgatcggcg taattggttc gttgcaggca atggaagcga
    21541 tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg tacgatgcaa
    21601 tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag gtgtgcgggc
    21661 agtaattgcc acctccaggc cagataagac gcggcactcc gccacgtcat cagcaattta
    21721 tgaggatgca gaacgtccgg ttaaatcgac gttctgccaa acgctcccag ccagtcctgt
    21781 tcaaacttcg ccacagcggc ttccaccgcc ggatagctaa tcatctgttg tgccacatcc
    21841 agtggcagcg taattgattc acatcctgcc agtaagcagt ccagcgcctg acgcggggtt
    21901 ttgaaactcg ctgccagcac tttcgcctgc ggcgcatgca ttttcaataa ctggtgtaag
    21961 tcggtcacag tctgaatgcc gctaccaccc tgagcatcaa tacgattaac gtaaggcgca
    22021 acatattccg cacctgccag cgccgacagc agcccttgtg ctgcgccata taccgcggtt
    22081 cccagcgttg gaatcccttc cgcttttaac atcttaatag ctgccagccc ctcggcggtc
    22141 accggaactt tcaccacgat atccgcaata atagaacgca atttacgtgc gtcattcacc
    22201 atcccttcag cagtggtagc cattacctgg gcaaacagac gcccctgacc gcccatcgct
    22261 tcatgaagtt gcggaagcac aacatccagc ggtttgttac ccgcggcgat aatgcttggg
    22321 ttagtggtca cacccgccag cggaaaaata cgtgacagcg ccttcaccgc aacaacgtct
    22381 gaagtatcca gatacagttc cataaccatc ctcaaaatgt actttttgtc tgaatgtaga
    22441 ctatcacggg aaaactctct gaacagttga cgcacatcaa tctaactttc attcgaaagc
    22501 attttaatgt ttgaacgaaa cagagaggca actatgattt tcaatattca gcgctactcg
    22561 acccatgatg gccccggtat ccgcacggtc gtatttctta aaggctgttc gctgggctgc
    22621 cgctggtgtc agaacccgga aagccgcgcc cgcacgcagg atctgctgta tgacgcacga
    22681 ctgtgtctgg aaggctgcga gctgtgcgct aaggccgcgc cggaagtgat tgagcgcgcg
    22741 ctgaatggtt tgcttattca tcgggaaaag ttaaccccgg agcatctgac ggcgttaacc
    22801 gactgctgtc cgacacaggc attaaccgtg tgtggtgaag tgaaaagcgt tgaggagatc
    22861 atgacgaccg ttctgcgcga taaaccgttt tatgatcgca gcggcggcgg tttaacgctt
    22921 tcgggtggtg agccctttat gcagccggaa atggcgatgg cgctactgca agccagccac
    22981 gaggcaggca ttcatactgc ggtagaaacc tgtctgcatg tgccgtggaa atatatcgcc
    23041 ccttctctgc cctatatcga tctgtttctt gccgatttaa aacacgttgc cgacgcgccg
    23101 tttaaacagt ggaccgacgg taacgccgcc agagtgctgg ataacctgaa aaaactcgcc
    23161 gcagcgggca aaaaaatcat tatccgcgtg ccgctgattc agggctttaa tgccgacgaa
    23221 acctctgtaa aagccattac cgattttgcc gccgacgagc tgcacgttgg cgaaattcat
    23281 tttctgccct accacacgct gggcatcaac aaatatcact tacttaatct gccctatgac
    23341 gccccggaaa aaccgcttga tgcgccagaa ctgctcgact ttgcccagca gtatgcctgc
    23401 cagaaagggt taaccgcgac cttacgagga taacaaccat gaccacactg aaactggaca
    23461 cgctcagcga ccgcattaaa gcgcacaaaa atgcgctggt gcatattgtg aaaccgccag
    23521 tctgtaccga gcgcgcgcag cactataccg agatgtatca acaacatctc gataagccga
    23581 tcccggtacg tcgcgcgctg gcactggcgc atcacctggc gaatcgcacc atctggatca
    23641 aacacgatga gttgatcatt ggcaaccagg caagcgaagt tcgcgccgcg ccgatcttcc
    23701 cggaatatac tgtctcgtgg atcgaaaaag agattgatga tctggcagat cgtcccggtg
    23761 ctggctttgc ggtgagcgaa gagaacaaac gcgttctgca tgaagtgtgc ccgtggtggc
    23821 gcggtcagac cgtacaggat cgctgctacg gcatgtttac cgatgagcaa aaaggtctgc
    23881 tggcgaccgg aatcattaaa gcggaaggca atatgacctc cggcgatgcg cacctggcgg
    23941 tgaatttccc gctgctgctg gaaaaaggtc ttgatggtct gcgtgagaaa gtagcggaac
    24001 gtcgctcgcg catcaatctg acggtgctgg aagatttaca tggcgaacaa ttcctgaaag
    24061 cgattgatat cgtgctggtg gcagtcagtg aacacattga acgtttcgct gccctggcgc
    24121 gtgaaatggc tgctaccgaa actcgcgaaa gccgtcgcga tgaactgctg acgatggcag
    24181 aaaactgcga tcttatcgcc caccagccgc cgcagacttt ctggcaggcg ctgcaactgt
    24241 gttacttcat ccaattgatt ttgcagatcg aatctaacgg tcactcggta tcgtttggtc
    24301 gtatggacca gtatctctac ccgtactatc gccgcgacgt tgaactcaac cagacgctgg
    24361 atcgcgaaca cgccatcgag atgctgcata gctgctggct gaaactgctg gaagtgaaca
    24421 agatccgctc cggctcacac tcaaaagcct ctgcgggaag tccgctgtat cagaacgtta
    24481 ccattggcgg acaaaatctg gttgatggtc aaccaatgga tgcagtgaat cccctctctt
    24541 acgcgatcct cgaatcctgc ggtcgcctgc gttccactca gcctaacctc agcgtgcgtt
    24601 accatgcagg aatgagtaat gatttcctcg acgcctgcgt acaggtgatc cgttgcggct
    24661 tcgggatgcc ggcgttcaac aacgacgaaa tcgtgatccc ggaatttatt aaactcggta
    24721 ttgaaccgca ggacgcttac gactacgcag cgattggttg tatcgaaacc gccgtcggtg
    24781 gcaaatgggg ctatcgctgt accggcatga gctttatcaa cttcgcccgc gtgatgctgg
    24841 cggcgctgga aggcgggcgt gatgccacca gcggcaaagt gttcctgcca caagaaaaag
    24901 cgttgtcggc aggcaacttt aacaatttcg atgaagtaat ggacgcgtgg gatacgcaaa
    24961 tccgttatta cacgcgtaaa tcaatcgaaa tcgaatatgt cgtcgacacc atgctggaag
    25021 agaacgtgca cgatattctc tgctcggcgc tggtggatga ctgtattgag cgagcgaaaa
    25081 gtatcaagca aggcggcgcg aaatatgact gggtttctgg cctgcaggtt ggcattgcca
    25141 acctcggcaa cagcctggcg gcagtgaaga aactggtatt tgaacagggc gcgattggtc
    25201 agcaacagct ggctgccgca ctggcggatg acttcgatgg cctgactcat gagcaactgc
    25261 gtcagcggct gattaacggt gcgccgaagt acggcaacga cgatgatact gtcgatacgc
    25321 tgctggctcg cgcttatcag acctatatcg acgaactgaa acagtaccat aatccgcgct
    25381 acggtcgtgg tccggttggc ggcaactatt acgcgggtac gtcatctatc tccgctaacg
    25441 taccgtttgg cgcgcaaact atggcaacgc cggacgggcg taaagcccac accccgctgg
    25501 cagaaggcgc tagtccggcc tctggtactg accatctcgg ccctactgcg gtcattggct
    25561 cggtgggtaa actgcctacg gcagcgattc tcggcggcgt gttgctcaac cagaaactga
    25621 atccggcaac gctggagaac gaatctgaca agcagaagct gatgatcctg ctgcgcacct
    25681 tcttcgaggt gcataaaggc tggcatattc agtacaacat cgtttcccgc gaaacgctgc
    25741 tggaggcgaa aaaacatccg gatcagtacc gcgatctggt agtgcgtgtc gcgggttatt
    25801 ccgccttctt caccgcgctc tctccagacg ctcaggacga tatcatcgct cgtactgaac
    25861 atatgctgta atctcttcgg cccggctgcg gcgtcgccgg gctaaatcat tcacatcatc
    25921 aatttcatcc ttactttcat tcgaaatata atttgtgctc tgcgtcacat tgttattaca
    25981 ctgttacggt ttcattttcg agccaggagc acacctatga gcgtaaaagt tatcgtcaca
    26041 gacatggacg gtacttttct taacgacgcc aaaacgtaca accaaccacg ttttatggcg
    26101 caatatcagg aactgaaaaa gcgcggcatt gagttcgttg tcgccagcgg taatcagtat
    26161 taccagctta tttcgttctt tcctgagcta aaggatgaga tctcttttgt cgcggaaaac
    26221 ggcgcactgg tttacgaaca tggcaagcaa ctgttccacg gcgaactgac ccgacatgaa
    26281 tcgcggattg ttattggcga gttgctaaaa gataagcaac tcaattttgt cgcctgcggt
    26341 ctgcaaagtg catatgtcag cgaaaatgcc cccgaagcat ttgtcgcact gatggcaaaa
    26401 cactaccatc gcctgaaagc tgtaaaagat tatcaggaga ttgacgacgt actgttcaag
    26461 ttttcgctca acctgccaga tgaacaaatc ccgttagtga tcgacaaact gcacatagcg
    26521 ctcgatggca ttatgaaacc cgtcaccagt ggttttggct ttatcgacct gattattccc
    26581 ggtctacata aagcaaacgg tatttcgcgg ttactgaaac gctgggatct gtcaccgcaa
    26641 aatgtggtag cgattggcga cagcggtaac gatgcggaga tgctgaaaat ggcgcgttat
    26701 tcctttgcga tgggcaatgc tgcggaaaac attaaacaaa tcgcccgtta cgctaccgat
    26761 gataataatc atgaaggcgc gctgaatgtg attcaggcgg tgctggataa cacatcccct
    26821 tttaacagct gacctctcta ccggagccgt gcgctccggt tttcccttct ttttacatcc
    26881 tgtacatcac atcaaatcat taaacttgca ttaacttatt tataacttat attgtcacat
    26941 gcaaatcaga atggtttcga atgaaagtta atgagaggtg gttatggctt ctacttttac
    27001 cagcgacaca ttgcctgccg atcacaaagc agctatccgt cagatgaagc acgcgctgcg
    27061 ggcgcagctt ggcgacgtcc agcagatctt taatcagcta agcgatgaca ttgccacgcg
    27121 agtggctgaa atcaacgcac tcaaagcaca gggcgatgcc gtctggccgg tgctgtctta
    27181 tgccgatatc aaagcaggtc atgttactgc agagcagcgc gaacagatta aacgtcgcgg
    27241 ttgtgcggtg ataaaaggcc atttcccccg cgaacaagcg ctaggctggg atcagtcgat
    27301 gctggactat ctggaccgca accgctttga cgaggtctac aaaggccccg gcgataattt
    27361 cttcgggacg ctcagcgctt cacgtcccga gatttacccc atctactggt cgcaggcgca
    27421 aatgcaggcc cgccagagtg aagaaatggc gaatgcgcag tcgtttctca atcgtctgtg
    27481 gacatttgaa agtgatggaa agcaatggtt taacccggat gtgagcgtca tctcccctga
    27541 ccgtatccgc cgccgtccgc ccggaacgac ctccaaaggt cttggagcgc ataccgactc
    27601 cggggcgctg gaacgctggc tgcttccagc gtatcagcgc gttttcgcca acgtctttaa
    27661 tggcaatctg gcgcaatatg atccctggca tgcggcacat cgtacggaag ttgaagagta
    27721 cacggtggac aacaccacca aatgttccgt gtttcggaca ttccagggct ggacagcgct
    27781 ctctgatatg ctgcctggtc aggggctgct gcacgttgtg cccattcctg aagccatggc
    27841 gtacgtactg ttacgtccgc tgcttgatga tgtgccggag gatgaactgt gcggcgtagc
    27901 gcccggaaga gtgttgccgg tatcagagca atggcatcca ctgttaattg aggcgttaac
    27961 cagcattcca aaactcgagg ccggagactc cgtctggtgg cactgcgacg tcatccattc
    28021 cgttgccccc gttgaaaatc aacagggttg gggcaacgtg atgtacattc ctgcggcacc
    28081 gatgtgcgag aaaaatcttg cctacgcgca caaggtgaag gccgcactgg aaaaaggcgc
    28141 atcgccgggc gacttcccgc gcgaggacta tgaaacaaac tgggaaggac gctttacgct
    28201 tgccgacctc aacattcacg gtaagcgagc gttgggcatg gatgtttgag agttaatgga
    28261 agtgaattga tgtgggatta agaaaatcga ttcgacgtta ctcagattgc tgacaagtgc
    28321 gctttcttaa tgcctgatgc gctacgctta tctggcctac aaagtcctgc agattcaata
    28381 tattacaggt ac
//
LOCUS       DAZGOB010000056        28326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGOB010000056 DAZGOB010000000
VERSION     DAZGOB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.056"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(70..1173)
                     /gene="gldA"
                     /locus_tag="ACHLIF_002569"
     CDS             complement(70..1173)
                     /gene="gldA"
                     /locus_tag="ACHLIF_002569"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO9606565.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1184..1846)
                     /gene="fsa"
                     /locus_tag="ACHLIF_002570"
     CDS             complement(1184..1846)
                     /gene="fsa"
                     /locus_tag="ACHLIF_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO9606566.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1858..4359)
                     /gene="ptsP"
                     /locus_tag="ACHLIF_002571"
                     /pseudo
     CDS             complement(1858..4359)
                     /gene="ptsP"
                     /locus_tag="ACHLIF_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            4668..5747
                     /gene="frwC"
                     /locus_tag="ACHLIF_002572"
     CDS             4668..5747
                     /gene="frwC"
                     /locus_tag="ACHLIF_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO9606567.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5762..6082
                     /gene="frwB"
                     /locus_tag="ACHLIF_002573"
     CDS             5762..6082
                     /gene="frwB"
                     /locus_tag="ACHLIF_002573"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO9606568.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6133..8430
                     /gene="pflD"
                     /locus_tag="ACHLIF_002574"
     CDS             6133..8430
                     /gene="pflD"
                     /locus_tag="ACHLIF_002574"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO9606569.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8396..9274
                     /gene="pflC"
                     /locus_tag="ACHLIF_002575"
     CDS             8396..9274
                     /gene="pflC"
                     /locus_tag="ACHLIF_002575"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO9606570.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDVPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9276..9617
                     /gene="frwD"
                     /locus_tag="ACHLIF_002576"
     CDS             9276..9617
                     /gene="frwD"
                     /locus_tag="ACHLIF_002576"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO9606571.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9604..10455)
                     /gene="yijO"
                     /locus_tag="ACHLIF_002577"
     CDS             complement(9604..10455)
                     /gene="yijO"
                     /locus_tag="ACHLIF_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9606572.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10604..12337)
                     /gene="eptC"
                     /locus_tag="ACHLIF_002578"
     CDS             complement(10604..12337)
                     /gene="eptC"
                     /locus_tag="ACHLIF_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO9606573.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12520..15171)
                     /gene="ppc"
                     /locus_tag="ACHLIF_002579"
     CDS             complement(12520..15171)
                     /gene="ppc"
                     /locus_tag="ACHLIF_002579"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO9606574.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15523..16674)
                     /gene="argE"
                     /locus_tag="ACHLIF_002580"
     CDS             complement(15523..16674)
                     /gene="argE"
                     /locus_tag="ACHLIF_002580"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO9606575.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16828..17832
                     /gene="argC"
                     /locus_tag="ACHLIF_002581"
     CDS             16828..17832
                     /gene="argC"
                     /locus_tag="ACHLIF_002581"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO9606576.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPCCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17840..18616
                     /gene="argB"
                     /locus_tag="ACHLIF_002582"
     CDS             17840..18616
                     /gene="argB"
                     /locus_tag="ACHLIF_002582"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO9606577.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18677..20050
                     /gene="argH"
                     /locus_tag="ACHLIF_002583"
     CDS             18677..20050
                     /gene="argH"
                     /locus_tag="ACHLIF_002583"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO9606578.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            20317..21234
                     /gene="oxyR"
                     /locus_tag="ACHLIF_002584"
     CDS             20317..21234
                     /gene="oxyR"
                     /locus_tag="ACHLIF_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO9606579.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(21217..22617)
                     /gene="sthA"
                     /locus_tag="ACHLIF_002585"
     CDS             complement(21217..22617)
                     /gene="sthA"
                     /locus_tag="ACHLIF_002585"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO9606580.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            22948..23598
                     /gene="fabR"
                     /locus_tag="ACHLIF_002586"
     CDS             22948..23598
                     /gene="fabR"
                     /locus_tag="ACHLIF_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO9606581.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFTSLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            23598..23957
                     /gene="yijD"
                     /locus_tag="ACHLIF_002587"
     CDS             23598..23957
                     /gene="yijD"
                     /locus_tag="ACHLIF_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO9606582.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(23997..25097)
                     /gene="trmA"
                     /locus_tag="ACHLIF_002588"
     CDS             complement(23997..25097)
                     /gene="trmA"
                     /locus_tag="ACHLIF_002588"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO9606583.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      25211..25401
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            25466..27310
                     /gene="btuB"
                     /locus_tag="ACHLIF_002589"
     CDS             25466..27310
                     /gene="btuB"
                     /locus_tag="ACHLIF_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO9606584.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            27255..28112
                     /gene="murI"
                     /locus_tag="ACHLIF_002590"
     CDS             27255..28112
                     /gene="murI"
                     /locus_tag="ACHLIF_002590"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO9606585.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6476 a         7124 c         7809 g         6917 t
ORIGIN      
        1 actgataagc gtagcgcatc aggctatttt gcgttcaaac tcccggacaa gccgggagtt
       61 tggagtaggt tattcccact cttgcaggaa acgctgaccg tactggtcgg ctaccagcag
      121 agcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcaccttc
      181 tgcacatgcc gcttctgcca caattcgcat tttcgccggg acatcttctt taatatccag
      241 ttgagcgaga gttattggca aacctaccgc atggctaagt gcagctacgg tttcgatttc
      301 ctcaaccggc gcgttttcca gtaccagctg cgtcagcgta ccgaatgcca ctttttcacc
      361 gtgataatag tgatgcgcgt ccgggatagc ggtcaggccg ttatgcaccg catgagccgc
      421 cgccagtccg ccgctttcaa aaccaacgcc gcttaaatag gtgttcgctt caatcacgcg
      481 ttccagcgca ggggttacta catgctgttc ggcggcaagc atcgcttttt cgccttcttc
      541 cagcagagtg ttgtagcaca gttctgccag tgccagcgcc gcctgggtgc acttgccgcc
      601 cgccatagtg gtagcgccgc tacgggaaca ggcgcgtgct tcaaaccagg ttgccagcgc
      661 atcgccgata cccgccgcta acagacgtgc aggagcgccc gcgacgattt tggtgtcgac
      721 aatgaccata ttcgggttat ttggcaacag tagatagcgg tcaaactcac cctcatcggt
      781 gtagataaca gacaatgcgc tgcacggtgc atcggtagag gcgatagtcg gtgcgatcgc
      841 taccggaaca cccatgaaat gtgccagtgc tttggcagta tcgagggttt ttccgccacc
      901 gataccgaga attgcgccac actgcgcagt ctccgcgatg ccacgcagac gatcgatctc
      961 attttgcgaa cattcaccgc caaacggcgc aatttctact accagtccag catctttaaa
     1021 gcttttctcg acagtggatt gagcaaaacc taaaacaaat ttgtcaccca ccactaacca
     1081 gcgttctgcc agcggcttca ggtattcgcc cagacgatta atcacatcag cgccctggat
     1141 gtatttaccc ggtgattgaa taatgcggtc cataattgct cctttagaga tgagtagtgc
     1201 caaatgcggc attccagtcg tgttcgaact tctctatagc tgactctacc gcaggggtgt
     1261 tgagcatttg ttgcgctaca tctaaaggca gggtgatgga ctcacatcct gccagcaaac
     1321 aatccagtgc ctgacgcggc gttttaaagc tggctgcaag caccatgctt tctggcgcgt
     1381 gcatttctaa cagcgcttgc agctcctgaa ccgtacgaat gccgtccccg ccctgggcat
     1441 ctacgcggtt aacatacgga gcaacgtatt ttgcccctgc cagcgcggcc agtaaccctt
     1501 gtgcagcact gtacactgcg gtgccaagcg tagtaatacc ctcttttttc agcatcttaa
     1561 tggcggccag accttcggaa gtcaccggga ttttcaccac aatgcccgga atggcgtcgc
     1621 gcaggcgctt cgcttcttcc accataccct gcgcgtcacg actcatagtc tgagcaaaca
     1681 gaatgccctc atcaccaatc gctttttgca gacgcggcag aacttcccat atggactcct
     1741 tgctggcagc gataatgctc gggttagttg tcaccccggc aatggggaat atgcgtgcca
     1801 gacgttcgac ttctgcgacg ttagcggtgt ccagatacag ttccatgatg tttcctttta
     1861 cagttccagt tcatgttgca gcaggctggc gatagcgtcg gcagaggcgg cgttaaccag
     1921 cgcgttacgg aattcttcgt gcatgatgcg gcgagcgagg cgcgagaaaa tgcgcatgtg
     1981 ctgatcgccc gcagcgtgtt tgttcagggt taacataatg atgaattgcg cttcatcatc
     2041 gccccagcgc accggcgctt gcagacgcgc tacactgatg gtggattgct caatgtgttc
     2101 tgatttgctg tggggaatgg caaaactaaa gcccagaccg gtagagaaaa cggcctcgcg
     2161 cgcccacaag tcggcttcca gtttacgcgg atagcggcag cggcccgcca gcagcaggtt
     2221 atcggtcatg cctttgagca cttcttcttt gctgcgtcag tcgctttcca gcgtgatgca
     2281 ctcggcggtg accagcggtg cgtcctgttg ggtcatgcgg aattgcgcca gcaggtgttc
     2341 cacttccagc gaagtacggc aggccattgc ctggttgagc aactggcggc attcgcggct
     2401 atcaagttgc gccattcgcg ctttcgccgc cggaattgac ggtgcgctca tgctcagctc
     2461 atccagccct aaaccgacca gcaacggcag cacggaacct ttcgctccca gctcaccgca
     2521 cagaccaatc catttgccct ggcgatgcac cgcttgcacg gcgtaatcga gcgcccgcaa
     2581 gaatgccgga ttcaggctgt tgtagtgacg agtaacctta gcattatcgc gatccaccgc
     2641 cagcagatat tgcgtcaggt cattactacc aatactaaag aaatcaatct cttcgcagca
     2701 ttgatcgatg atgaacatca ccgacggcac ttccagcata atgccgagct ggatcttctc
     2761 atcaaacgga atgtgttcgt tacgcagttg ctgtttggct tccgccagtt tttctttcac
     2821 ccataagatc tcttccattg aagagatcat cgggatcatg attttcaggc tgccgtgagc
     2881 ggaggcgcgc aggatcgacc gtagttgtgt ggtgaacagc gaggcgtatt cttcataaat
     2941 acgcacagcg cgataaccga ggaacgggtt agcttctgcc ggaatgttca gataatcaac
     3001 gggtttgtcg ccgccaatgt ccatagtgcg cacaataatg ctgcgtccgt tggcagactc
     3061 cagcgcctga caaaaaatgt tgtacagctc gctttcgccc ggtgcactgg tgcgatccat
     3121 atagagcatt tcagtgcgga acaaaccaac gccttccgca ccattgccga atgcggcctg
     3181 cgcttccacg gagtgagcga tgttagcggc aatttcaatg cggataccgt cagcggtacg
     3241 ggcttgttgg gtcagccaga cacgctgttg ctcacgcagg gcatcctgta cgcgggcttc
     3301 ttgctgataa taacgggcta cggcttcccc tggctcaacc acaatcgctc cggcgttgcc
     3361 gtcgatataa atcgtttgat gttgccacgg ggtaagggca tcaatatcca caccaaccag
     3421 cgtcggaatg ttgaacgaac gggcaaggat caccgtatgt gaggtggtgc cgccgctttt
     3481 gagcaacaat cctttgaggt gatttttatc cagttcgagg aactggctgg gggtcagttc
     3541 atcagccata caaatggcgg gctgcgtcag tttgcccggt gccgggaagc gttgctcacc
     3601 gtagatttgc tggagtaact ggaagcagac gtcgcgtacg tccagggcac gttcttgcag
     3661 gtagctgctg ctggaacggg aaaactcttc acaaaagtga ttcgcactgg cgacaatcgc
     3721 ttcggcgcag cttaatccgg cgctgacgcc tgccagtaaa tgttcgtgca gggaagtatc
     3781 gccagccagg gatcggtgcg cttccagaat cgcgctggtg gcaccgtcgc tatccagcag
     3841 acgaaactca atgtttttca gtaccagcgt caggccgttt tccagtgcgg attgctcggc
     3901 gtcaacgtct ttggctgcgg gaagattact cagcgcattg agatctaaag aagagatcgg
     3961 cgtcagaatg ccgcctgcgc taccgctgca cacggtgcgg gcgcggataa tttgcggatt
     4021 cagattggtc agtgaaaccg gcagtggttc cagttcgtca gatttaactt ccgccagcgg
     4081 tgcgtcgcag tgggggaatt catcgcgcag ccattggctt aaacgctggt gcgcttcctg
     4141 ttcgtcggcc ccggaaatca gtaaccggca gttatcgccc gccagcgtgt cggtgccaat
     4201 cagcgcaagg gcgcttttgg cgttgccctt gcggtcagtg cgcaggttat gccactcaat
     4261 ttgtgatgaa aaagtattac acagcgtttc aacgtggctt gccggacgtg catgtacgcc
     4321 gttaggtagc tcacaaataa attccacaat cagggccatt gcctctctcc cggtacggtt
     4381 ttctgtttac aggataagag cgcacggcaa cggcctgcca tgtgacaaat ctgccaaaag
     4441 ctggacaaat gtaatgtaac cgtcaatttg cgacgcgtct cacaagacgc cgttttgcgg
     4501 catgcttccg gattatcgca agttatgagg cggatcgcat ttttgtactg atattacaaa
     4561 agtccagtaa atggcctttt tatccactgt ttgctccgcc tgcgattgcc tattgtttcc
     4621 tccatcaaaa atatggacat gggctttcgc aagcccggga gcagcttatg aatgagttgg
     4681 tgcagatcct gaaaaatacc cgtcagcatt taatgacggg cgtttcacac atgattccct
     4741 tcgtggtatc gggcggtatt ttgctggcgg tttccgtcat gttgtatggc aaaggcgcag
     4801 tgccggatgc cgtagccgat ccgaatctga aaaaactgtt tgatatcgga gttgcaggtt
     4861 tgacgctgat ggtgcctttc ctcgccgctt acatcggtta ctccattgca gagcgttctg
     4921 cgctggcccc gtgcgctatc ggtgcctggg ttggtaacag ctttggtgcg ggcttctttg
     4981 gtgcgctgat cgccgggatt atcggcggca tcgtggtgca ttacctgaag aaaattccgg
     5041 tgcataaagt tctgcgctcg gtgatgccta tcttcattat tcctatcgtc ggcacactga
     5101 ttaccgcagg catcatgatg tggggcttgg gcgagcctgt aggggcgttg accaacagcc
     5161 tgactcagtg gcttcagggg atgcagcagg gcagcattgt tatgctggcg gtgatcatgg
     5221 gtctgatgct ggcgttcgat atgggcggtc cggttaacaa agtggcctat gccttcatgc
     5281 tgatttgcgt tgctcagggt gtttataccg tggtggccat tgctgccgtg ggtatttgta
     5341 tcccgccgct ggggatgggg ctggcgacgc tgattggtcg taaaaatttc tccgcagaag
     5401 agcgcgaaac cggcaaagcg gcgctggtga tggggtgcgt tggggttact gaaggggcga
     5461 ttcctttcgc cgctgccgat ccgctgcgtg tcattccttc catcatggtc ggttctgttt
     5521 gtggtgcggt aactgcggcg ctggtcggtg cgcagtgcta tgcaggctgg ggtggtctga
     5581 ttgtgctgcc ggtggttgaa ggcaagctgg gttatatcgc agcagtggct gtcggagcag
     5641 tggtgacggc tgtttgcgtg aacgtgctga aaagtctggc gcgtaaaaat gggtcttcga
     5701 ctgatgagaa ggaagacgac ctggatttgg attttgaaat taattaattg aggaacgagc
     5761 catgacgaaa attattgcag taaccgcgtg tccatctggt gttgcccata cctatatggc
     5821 ggcagaggcg ctggaaagcg ccgcgaaagc gaaaggctgg gaagtgaaag tagaaactca
     5881 ggggtcaatt ggtctggaaa acgaactgac tgcggaagac gtggcgagcg ccgatatggt
     5941 gattctgacc aaagatatcg gcatcaagtt tgaagagcgt tttgctggca agaccatcgt
     6001 gcgcgtcaac atcagcgatg cggtaaaacg tgctgacgcc attatgagca aaattgaagc
     6061 tcacctggcg caaaccgctt aagttcttct cccgctcgca agggcgggtt cgctttccca
     6121 caggagttcc tcatgacgaa tcgtatctct cgcctcaaaa ctgcactgtt ttccaatacc
     6181 cgtgaaatct cgctggagcg ggcgctgctt tataccgcca gccatcggca aaccgaaggc
     6241 gaaccggtga tattgcgccg ggcgaaagca acagcgtata tccttgaaca tgttgaaatt
     6301 tcgattcgtg atgaagaact gattgccggt aaccgcaccg taaaaccgcg cgccgggatt
     6361 atgtcgccgg aaatggaccc ttactggctg ctgaaagagc tggatcaatt cccgacgcgt
     6421 ccgcaggacc gctttgctat cagcgaagaa gataaacgta tctaccgcga agagttgttc
     6481 ccgtactggg aaaaacgttc gatgaaagat ttcatcaacg ggcagatgac agatgaagta
     6541 aaagccgcga ccagcacgca gattttcagc attaaccaga cggataaagg ccaggggcac
     6601 attattattg attacccacg cctgctgaat cacgggctgg gggagctggt ggcgcagatg
     6661 cagcaacatt gtcagcaaca gccggagaat cacttttatc aggccgcgct gttactgctg
     6721 gaagcctcgc agaaacacat tctgcgttac gctgaactgg cggaaacaat ggcggcaaac
     6781 tgcacagatg cccagcgtcg cgaagaactg ctgactattg cggagatctc ccgccataac
     6841 gccgaacata agccgcagac gttctggcag gcgtgccagt tattctggta catgaacatc
     6901 attctgcaat acgaatccaa cgccagttcg ctgtctttag ggcgcttcga ccagtatatg
     6961 ttgccgttct atcaggcttc tttaacccag ggcgaagatc cggcgttcct gaaagaactg
     7021 ctcgaatctt tatgggtgaa atgcaacgac atcgtgctgt tgcgctccac cagcagcgcg
     7081 cgttatttcg caggtttccc gaccggctat accgcactgc tcggcgggtt aaccgagaac
     7141 ggacgtagcg cggtgaacgt gctttcgttc ctttgccttg acgcctatca aagcgtgcaa
     7201 ttaccgcaac cgaacctcgg cgtgcgcact aacgccttga tcgacacgcc gttcctgatg
     7261 aaaaccgccg aaaccattcg cctcggcacc ggtattccgc aaatctttaa cgatgaagtg
     7321 gtggtaccag cgttcctcaa ccgtggcgtt tcgctggaag atgcgcgcga ctattccgta
     7381 gtgggctgtg tggaattatc tattcccggc agaacctacg gcttgcatga catcgcgatg
     7441 tttaatctgc tgaaagtgat ggaaatctgc ctgcatgaaa atgaaggcaa tgctgcgctg
     7501 acttatgaag gtttactgga gcagatccgc gccaagatca gccactacat caccctgatg
     7561 gttgagggca gcaatatttg tgatatcggc catcgcgact gggcacctgt accgctgctc
     7621 tcgtctttta tcagcgattg tctggaaaaa ggtcgcgata ttaccgatgg cggcgcgcgt
     7681 tataacttct ccggcgtaca ggggatcggt atcgccaacc tgagcgattc tctccatgcg
     7741 ttgaaaggga tggtttttga gcaacagcgt ttaagttttg acgaattgct gtcggtatta
     7801 aaagccaact ttgcaacgcc agaaggcgaa aaagtccgtg ctcgcttaat taaccgcttc
     7861 gagaaatacg gtaacgatat cgacgaggtg gataacatca gcgccgaact gttacgccac
     7921 tactgcaaag aagtggaaaa ataccagaac ccgcgcggtg gttacttcac gccgggatcg
     7981 tataccgttt ctgctcacgt cccgttggga tcggtggttg gcgcgacgcc agacggtcgt
     8041 tttgccgggg aacaactggc agacggcggc ttgtcaccta tgctgggcca ggacgcacaa
     8101 gggccaacag cggtactgaa gtcagtcagt aagctcgata atacgctgct gtctaacggt
     8161 acgttgctga acgtgaaatt cactccggcg accctggaag gtgaagcggg attacgcaaa
     8221 ctggccgact tcttacgggc gtttacccag cttaagttgc agcatattca gtttaacgtg
     8281 gtgaacgccg acacgttgcg ggaagcgcaa cagcgcccac aagattatgc cgggctggtg
     8341 gtgcgcgttg ccggatacag cgccttcttt gtcgaactgt cgaaggagat ccaggatgac
     8401 atcatccgcc ggacagcgca tcagctgtaa cgttgtggaa acgcgccgca atgatgtggc
     8461 gcgcattttc aacattcagc gttattcact gaatgacggc gagggcattc gtacggtggt
     8521 cttttttaaa ggctgtccgc atctttgccc atggtgtgct aatccggagt cgatctccgg
     8581 caaaatccag acggtacgca gagaggcgaa atgtctgcac tgtgcgaaat gtttgcgtga
     8641 tgcggatgaa tgcccctccg gggcgtttga acggattggt cgcgatatca gccttgacgc
     8701 gctggaacgg gaagtgatga aagatgacat tttctttcgc acgtccggcg gcggcgtcac
     8761 gctttctggc ggcgaagtgt taatgcaggc ggagtttgct acccgttttt tacagcggct
     8821 gcggttgtgg ggcgtgtcat gcgccattga aactgccgga gacgtgccag ccagcaagct
     8881 gttaccactg gcgaaattgt gcgatgaagt gctgttcgat ttaaaaatta tggacgcgac
     8941 tcaggcgcgg gatgtggtga agatgaacct gccacgcgtg ctggagaatc tgcgtttgct
     9001 ggtgagtgag ggtgtcaacg tgatcccgcg tttaccgttg atccctggtt tcacgctcag
     9061 ccgggagaat atgcagcagg cgctggatgt actgatcccg ctgaaaatca ggcagatcca
     9121 tctgttaccg tttcatcagt acggcgaacc gaaataccgc ctgctgggga aaacatggtc
     9181 gatgaaagag gtgcctgcgc cgtcgtcagc cgatgtgaca acgatgcgcg aaatggcaga
     9241 acgggccgga tttcaggtta ccgtgggagg ttaaaatggc atacctggtg gcagtaaccg
     9301 cctgcgtaag cggcgtggcg catacttata tggcggcgga acggctggaa aagttgtgcc
     9361 agttagagaa gtggggcgtt agcattgaaa ctcagggcgc gctgggaacg gagaatcgtt
     9421 tagcggacga ggatatccgt cgggcggatg ttgctctgtt gattacggat atcgagcttg
     9481 ccggtgccga gcgatttgaa cattgccgct atgtgcaatg cagcatctac gcattcctgc
     9541 gtgagccgca gcgggtaatg agcgcggtgc gcaaagtgct ttctgcgccg cagcaaaccc
     9601 atcttattct ggagtagtcg gtttttctgt cagttggctg tggtactgcc gacgatattc
     9661 cgacggcgag cgttcggtgt ttttacgaaa cagacggcag aagtagttgc tgtcgacaaa
     9721 gccgcaggcg tgcgccactt cttttacctt caggtcatag cctttcaata acgtcttagc
     9781 gtgctccagt cgcgtgtgat tcaggtattc gttaaaacca atggcccccg ttttttgaaa
     9841 caggtgcgag agatagtttg gtgaaatata gaatgcctgt gcgacagatt cgcgggtaag
     9901 cgcggaggca tagcgttcgt cgatataatc gcgaatagct tcaaatagtg cctggctgcg
     9961 tgaggcggtc tggatttggc tgccgagcaa atcgcggcag tggctaagca ggctggcgac
    10021 gataagcctt gccgtttgct gctcctgcgg ctgcatttgc atttcattga gcgtttgtag
    10081 cagaaaagaa cctatgcgtg ggccgcgccg ggcgacgtgt tgcttcgcca gattttgata
    10141 ttgtttgcca tcccattgca cgacgctgaa gccgagttgc tgtttgccaa acagcacgct
    10201 aaaggtagta gcgggggctt gccattgtgg aaaattccag ccaccagcag gaacgtacag
    10261 cacatcgccg ggaactaacg ttgctccagc gccggtatca acaaactcac cttccagcac
    10321 aatttccagc cgtggaaaat cgacctgata cgcgagatcg ggcaccgggc cgttactgct
    10381 ggcaaagtaa atctggcgca gggacagcgg gccgttgatc aggcgggaga gcagatagct
    10441 gacgtcgtga tacatgttat tcccttaggt atgtagacgg gaaaacagta actggcatta
    10501 ttaaaaaagg ccacctgaaa agtgtggcct gaggggagtt cgatgtaaac attgcggcag
    10561 tatcgcatcc ggcaaatagc gccagacgcg atacaaaggt catttactga ttacccacct
    10621 gatcgccata cggcagcgtg tcgtaatcga tcagcgcgtt tttcttgtac gggttaccaa
    10681 tccagcgggt agtttctttg aactgcggat tcaccactga acgggttgga tcgtaaccgt
    10741 cgtaagataa gcctgctaaa tctgaccagg tgtggatcag ttccgccagg ctgtatttac
    10801 gatcaacatc ctgcgagaaa tcacgcggat gagtcgcttg ccatttttcc gacgtccaca
    10861 gcaggaacgg aatggtgtac atgtggcgcg tcgggttgtc ttcgttacgc ccctgagttt
    10921 tgtgcggcgg cgtgtcgtaa acctcttcac cgtggtcaga gaaataaacc aggaaaccgt
    10981 tcgggtttgc tgctttaaag tctttaatca ggctggcaac cacatgatcg ttatacaggt
    11041 tagcgttgtc ataatcgtta tatgactcca gctcttccgc gtttaatcca ggcggaacat
    11101 gatcggtatt gccatcaaac ttgccctgat tttccgggta gcggtatttg tatttgatat
    11161 gcgtacccag cagatgaacg atgatcagtt tcttcggcgc cgggtccttc agcacttcct
    11221 ggaatggttt cagcacgttg gtgtcgtatt cacgcgcact ctgcgtacgt tgctggttca
    11281 tatagaactg tttgtcggtc tgcttcgaaa ataccgtcag catggtattg cgggcggtca
    11341 tcgtctgctg gttggtgatc cagaaggttt tataacctgc ctgtttcatc atgttcatca
    11401 gcgacggttg tgtcaggtac aggtccgggt tcttttcatt agcaaaggtc agcgcctgtt
    11461 gcaggatttc aatggtgtac ggacgagacg taaccacgtt attaaacacg gtcagattcg
    11521 gatcggtttt atgcagcgca tccagctccg gcgtggtttc acgcggataa ccgtacagac
    11581 tcatgcgccc gcgttgggtc gattcgccaa tcaccagcac taaggtacgc ggttcgttac
    11641 ccgattcatc tttgaaatta gccagaggcg gcaaggcatt attttcattc agtaactttg
    11701 ttagcgagtt tagttgctga cgatactgat aatagccggt caggaattgc cacggtgcgg
    11761 caggctccat acgcgaggcc aggttatcca acgttttctc aaacggcttg tttttgataa
    11821 acgtattcat ggcgatcgga tgcagaatca agccataaag cagggcaaaa gagacaacat
    11881 aacgccacgg ctttggaata tagaccgggt gcaggcgtgt ccacagcaga actgccaccg
    11941 ccgtataggc cagcgcgata agcacaattt tcaggctgaa atactggctt aaatactcgc
    12001 tggcttcgtt ggtgttggtt tcgaacatca caaacagaac gctctgcgag aactcctgcc
    12061 cgtagatgac gtagtagcat agcgccgcca gagaggccgc ccatagcacc acgccgatta
    12121 ctgcggcaat aattttaatc cgcttcggaa agaggaatac cgggatcaac cacagcgagc
    12181 tgaataacag cgagtcgcga atgccgttgg tgccactata accactgatg taaataatgg
    12241 cctgtagcag agtagagaaa aaccaaaagt agagcagtgc ccaacccagg gctttccagc
    12301 taaaaagggg tttagcctgg acttctgtgg aatgcatagt aagaacctgt cttgaaaaaa
    12361 atatcgccga atgtaacgac aattccttaa ggatatctga aggtatattc agaatttgaa
    12421 taaaatgcag acagaaatat attgaaaacg agggtgttag aacagaagta tttcagaaaa
    12481 ccctcgcgca aaagcacgag ggtttgcaga agaggaagat tagccggtat tacgcatacc
    12541 tgccgcaatc ccggcaatag tgaccattaa cgcttgttcg acgcgaggat ccggttcctg
    12601 gccttctttt tctgcctggc gggagcggtg cagtaactcg gcctgcaata cgttcagcgg
    12661 gtcggtgtaa atattccgta gctgaataga ctctgcaatc cacggcagat cggccattag
    12721 atgggaatcg ttggcaatcg ccagcaccac tttgatgtct tcttcttgca ggttgcgtaa
    12781 ctctttacct aacggccaca gtgctttgtc taccaggcgt tggtcatagt attccgccag
    12841 ccacaggtct gctttggcga agaccatctc cagcatgccg agacgcgtcg agaagaatgg
    12901 ccaatcgcgg cacatggctt ccagctcgct ctgtttgcca tcttcgacca ctttttgcag
    12961 cgccgtacct gcacccagcc aggcggggag catcaggcgg ttttgcgtcc aggcaaaaat
    13021 ccacggtatg gcgcgcagcg actcgacgcc gccggttggg cgacgtttcg ccggacgtga
    13081 acccaacggc agtttgccca attcttgttc cggcgtagcg gagcggaagt aaggcacaaa
    13141 atctttgttt tcacgtacgt agccacggta gagatcgcag gagatgactg acagttcatc
    13201 cataatgcga cgccagctct ctttcggctc aggtggtggc agcaggttgg cttccagaat
    13261 agccccggtg tacagcgaca ggctgctgac ggtgatttct ggcagaccat atttaaagcg
    13321 gatcatctcg ccctgttcag tcacgcgcag gccacctttc aggcttcctg gcggttgtga
    13381 cagcagcgcc gcatgagcag gtgcgccgcc gcgaccaatg gaaccgccgc gaccgtggaa
    13441 caacgtcagc tcaatacccg ctttttcgca ggttttgatt aatgcatcct gtgcctgata
    13501 ttgcgcccag gaagctgcca tcactcccgc atcttttgct gagtcggaat agccaatcat
    13561 caccatctgt ttgccctgaa tcaggccacg ataccagtca atattgagca gctgggtcat
    13621 gacatcgttg gcgttgttca gatcatcgag ggtttcaaac agcggagcaa ccggcatcgc
    13681 aaacccgata cccgcttctt tcagcagcag gtggacagcc agtacgtcgg acggcgtttt
    13741 cgccatcgag atcacgtagg cggcaatgga gccttgcggt gcttcggcaa tcacctggca
    13801 ggtatcgagc acttcgcgcg tttcggcgct tggttgccag ttgcgcggca gaagcggacg
    13861 tttggagttc agttcgcgga tcaggaacgc ctgtttgtcg gcctctgacc agctttcgta
    13921 gtcgccgata ccgaggtagc gggtcagctc gcccagcgct tcggtatgac gcgtgctctc
    13981 ctgacggata tcaatacgga ccagcggtac gccgaaacat ttcacgcggc gcagggtgtc
    14041 gagcagatcg ccgttggcga taatacccat gccacacgcc tgaagtgact ggtagcaagc
    14101 gtagagcggt tcccacagtt cttcgttttg tgtcagcagg ccttctggtt ttggcagttc
    14161 ttcgcctttc aggcgcgctt ccagccatgc ctgtgtcgcc atcaggcgag aacgcaggtt
    14221 tttcatcaga tagcgatacg gttctgcggc accttcttcg ccaaccagcg ccagcagttc
    14281 aggggtcgct tcaaccatcg acagttcaga aaccagcacc tgaatatctt tcaggaacaa
    14341 atcggtggct ttccagcggc tgagtagcag gacgtggcgg gtgatatcgg cagtgacgtt
    14401 cgggttgccg tcgcggtcgc cgcccatcca cgaagtaaaa cggaccggaa caaattcgac
    14461 gggcagtttg tagccgaggt tctcttccag ttgttcgttc agttcgcgca ggtaatttgg
    14521 tacgccttgc cacaggctgt tttccactac ggcaaagccc catttggctt catctaccgg
    14581 gcttggacgc agcttacgga tttcatcggt atgccatgac tgggcgatta actggcgcag
    14641 gcggcgcatc agctggttgt gttcgtagtc agcgatatct ttgttatcga gctgttttaa
    14701 acaggcgttc acttccacca ttttgtggat cagtgtacga cgggtaattt cggttgggtg
    14761 agccgtgagg accagttcca gcgacagcga ttccactgct tttttgatgg tgtcttcgct
    14821 cagttccggc tggtttttca gtttacgcag ggtgcgggcg atcacttccg ggttgctggc
    14881 agcttcgcct ttcggcgaaa tgctgtggta ttgctcggcg gtgttggcca ggttcaggaa
    14941 ctgactaaac gcacgcgcaa cgggcagcag ctcgtcgttc gacaaatttt gtaaggtggt
    15001 gagcaactcc tggcggttag catcattgcc agcgcgtgaa gatttcgaca acttacggat
    15061 agtttctacg cgttcaagaa tgtgttctcc caacgcatcc ttgatggttt ctcccagcac
    15121 tttgccgagc atactgacat tactacgcaa tgcggaatat tgttcgttca tattacccca
    15181 gacaccccat cttatcgttt gatagccctg tatccttcac gtcgcattgg cgcgaatatg
    15241 ctcgggcttt gcttttcgtc gtcttttata aagccacgta aaagcggtga cgtcaaatgc
    15301 tgcgaaatcg cttcagcaaa cgaataaata gcaggaattt acgccattaa attcacgacg
    15361 ctttaaataa gcgtaactta tggaaatgtt aaaaaatcgc catacataac accaaaggtg
    15421 taggtcggat aagatgcgca agcatcgcat ccgacattat tgcggcacag gagtttggca
    15481 acggtgccgg atgcggcgcg agcgccttat ccggcctacg ttttaatgcc agcaaaaatg
    15541 gtgaattacc tgggttatca gttcgcgggt gggcttgata aaccgtgttt ccagatattc
    15601 atcaggttga tgagcctgat taattgagcc aggccccaac accagcgtcg ggcataacgt
    15661 ttgaataaac ggcgcttcgg tacagtagtt caccacttcg gtctttgctc cgagcaattt
    15721 ctcaactact tcaaccagtt gatgattcgg tgggcattca tagccaggga tcggcggatg
    15781 cagctcgtcg accgtcagac gacccggcca gcgttcgctc accggagcca atgcatcgtt
    15841 gagcaaacca ttaagttcat tgagtgtcat gccaggcagc ggacgaatat ccatatgcaa
    15901 ctcacagcaa gcgcaaatac ggttagaagc gtcgccaccg tgaatatgcc cgaggttgag
    15961 cgtagggtat ggcacggtaa acgcttcgta gtgataacgt tctttcaggt tatcgcgcaa
    16021 ttgcaaaata tgcccgatgg cgtcgtgcat tagttcgata gcgttaactc cgcgtgctgg
    16081 atcgctggag tgccccgact ggccctgaat acggatggcg ttagagatat gacctttatg
    16141 tgcgcgtacc ggttgtagtg acgtcggttc gccaatgatg gcgcaatccg ggcgcagggc
    16201 ggtagtttcg gcaaaataac gcgctccggc catactggtt tcttcatcag cagtcgccag
    16261 aatgtagagc ggttttttca gtttcgtgac gtcgacatcg cgtagcgcat caaggataaa
    16321 cgcaaaaaag cctttcatgt cggcggtgcc taagccgtaa agcttgccgt catgctccgt
    16381 cagtgtaaac ggatcgcgcg tccagcgacc gtcatcaaat ggcaccgtat cggtatgccc
    16441 cgccagcaac aagccgccag ccccctgtcc gatacttgcc agcatattga atttgttgcg
    16501 agttcctggc acaggctgca cttccacatt gaagcctaaa tctttaaacc agtccgccag
    16561 cagagtgatt aaatctgcat tgctttgatc gagtgcctct tccgtggcgc ttattgaagg
    16621 tgtggcaatc agagcacggt aaatctcgat aaatggcggt aatttgtttt tcattgttga
    16681 cacacctctg gtcatgatag tatcaatatt catgcagtat ttatgaataa aaatacacta
    16741 acgttgagcg taataaaacc caccagccgt aaggtgaatg ttttacgttt aacctggcaa
    16801 ccagacataa gaaggtgaat agccccgatg ttgaatacgc tgattgtggg tgccagcggc
    16861 tacgctggcg cagagctagt gacctatgta aatcgccatc cgcatatgaa cataaccgct
    16921 ttgactgttt cagcgcaaag caatgatgcg ggaaagttaa tctccgattt gcatccgcag
    16981 ctaaaaggca tcgttgatct gccgttgcag ccgatgtcgg atatcagcga gtttagccca
    17041 ggggtggacg tagtgtttct cgccaccgcc catgaagtta gccacgattt agcgccgcag
    17101 tttcttgaag cgggctgcgt ggtgttcgac ctttccggcg cgtttcgtgt taacgacgcc
    17161 accttctatg aaaaatatta cggctttacc catcaatacc cggaactgtt ggaacaggca
    17221 gcctacggtc tggcggagtg gtgcggtaat aaattaaaag aagcgaattt gattgcggtg
    17281 ccgggctgtt atccgacggc ggcacagctg gcgctgaaac cgttgattga tgccgatctt
    17341 cttgacctca atcagtggcc ggtgatcaac gccaccagcg gcgtgagcgg tgcaggacgt
    17401 aaggcagcga tttcaaatag tttttgtgaa gttagcctgc aaccgtatgg cgtctttacc
    17461 catcgccatc aaccagagat cgccacacac ctcggtgctg acgttatctt caccccgcat
    17521 ctgggcaatt tcccgcgcgg cattcttgaa accattacct gccgcctgaa accgggtgtg
    17581 acccaggcgc aagtggcgca agcgttacag caggcgtatg cccataaacc gctggtgcgg
    17641 ctgtatgaca aaggcgttcc ggcgctgaaa aatgtcgttg ggctgccatg ttgcgatatc
    17701 gggtttgccg ttcagggcga gcatctgatt attgtggcga ccgaagacaa cttactgaaa
    17761 ggcgcggcgg cacaagcggt acagtgcgcc aatattcgtt tcggctatgc ggaaacgcag
    17821 tctcttattt aagggtgcaa tgatgaatcc attaattatc aaactgggcg gcgtactgct
    17881 ggatagtgaa gaggcgctgg aacgtctgtt tagcgcactg gtgaattatc gtgagtcaca
    17941 tcagcgtccg ctggtgattg tgcacggcgg cggttgcgtg gtggatgagc tgatgaaagg
    18001 gctgaatctg ccggtgaaaa agaaaaatgg cctgcgggtg acgcctgcgg atcagataga
    18061 cattatcacc ggagcactgg caggaacggc aaataaaacc ctgctggcat gggcgaagaa
    18121 acatcagatt gcggccgtag gtttgtttct cggtgacggc gacagcgtca aagtgaccca
    18181 gcttgatgaa gagttaggtc atgttggact ggcgcagcca ggttcgccta agcttatcaa
    18241 caccttgctg gagaacggct atctgccggt ggtcagctcc attggcgtaa cagacgaagg
    18301 gcaactgatg aacgtcaatg ccgaccaggc ggcgacggcg ctggcggcaa cgctgggcgc
    18361 ggatctgatt ttgctctctg atgtcagcgg cattctcgac ggcaaagggc aacgcattgc
    18421 cgaaatgacc gccgcgaaag cagaacaact gattgagcag ggcattatta ctgacggcat
    18481 gatagtgaaa gtgaacgcgg cgctggatgc tgcgcgcacg ttgggccgtc cggtagatat
    18541 cgcctcctgg cgtcatgcgg aacagcttcc ggcactgttt aacggtatgc cgatgggtac
    18601 gcggatttta gcttaagttt tgttggccgg aggcgcagct ttccggcatt gaatttcaaa
    18661 ataaggaaac agagttatgg cactttgggg cgggcgtttt acccaggcag cagatcaacg
    18721 gttcaaacaa ttcaacgact cactgcgctt tgattaccgt ctggcggagc aggatattgt
    18781 tggctctgtg gcctggtcca aagccctggt aacggtcggc gtgttaaccg cagaagagca
    18841 ggcgcaactg gaagaggcgc tgaacgtgtt gctggaagat gttcgcgcca ggccacaaca
    18901 aatccttgaa agcgacgccg aagatatcca tagctgggtg gaaggcaaac tgatcgacaa
    18961 agtgggccag ttaggcaaaa agctgcatac cgggcgtagc cgtaatgatc aggtagcgac
    19021 agacctgaaa ctgtggtgca aagataccgt tggcgagttg ctgacggcta accggcagct
    19081 gcaatcggcg ctggtggaaa ccgcacaaaa caatcaggac gcggtgatgc caggttacac
    19141 tcacctgcaa cgcgcccagc cggtgacgtt cgcgcactgg tgcctggcct atgttgagat
    19201 gctggcgcgt gatgaaagcc gtttgcagga tgcgcttaag cgtctggatg tcagcccatt
    19261 aggctgtggc gcgctggcgg gaacggccta tgaaatcgac cgtgaacagt tagcaggctg
    19321 gctgggcttt gcttcggcga cccgtaacag tctcgacagc gtttctgacc gtgaccatgt
    19381 gttggaactg ctttctgctg ccgctatcgg catggtgcat ctgtcgcgtt ttgctgaaga
    19441 tctgattttc tttaacaccg gcgaagcggg gtttgtggag ctttctgacc gcgtgacttc
    19501 cggttcatca ttaatgccgc agaagaaaaa cccggatgcg ctggagctga ttcgcggtaa
    19561 atgcggtcgg gtgcaggggg cgttaaccgg catgatgatg acgctgaaag gtttgccgct
    19621 ggcttacaac aaagatatgc aggaagacaa agaaggtctg ttcgacgcgc tcgatacctg
    19681 gctggactgc ctgcatatgg cggcgctggt gctggacggc attcaggtga aacgtccacg
    19741 ttgccaggaa gcggctcagc agggttacgc caacgccacc gaactggcgg attatctggt
    19801 ggcgaaaggc gtaccgttcc gcgaggcgca ccatattgtt ggtgaagcgg tggtggaagc
    19861 cattcgtcag ggcaaaccgc tggaagatct gccgctcgac gagttgcaga aattcagccc
    19921 ggtgattgac gaagatgtct atccgattct gtcgctgcaa tcgtgcctcg acaagcgtgc
    19981 ggcaaaaggc ggcgtctcac cgcagcaggt ggcgcaggca attgcttttg cgcaggcgcg
    20041 gttggggtaa gaacatttat atgtataaat ttgagcctgg cttatcgccg ggctttttta
    20101 tggcaaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt agttattcga
    20161 gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc cgctccgttt
    20221 ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc tattctacct
    20281 atcgccatga actatcgtgg caatggagga tggataatga atattcgtga tcttgagtac
    20341 ctggtggcat tggccgaaca ccgccatttt cggcgtgcgg cagattcctg ccacgttagc
    20401 cagccgacgc ttagcgggca aattcgtaag ctggaagatg agttgggcgt gatgttgctg
    20461 gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt agatcaggcg
    20521 cgtaccgtac tgcgtgaggt gaaagtcctt aaagagatgg caagccagca gggcgagacg
    20581 atgtccgggc cgctgcacat tggtttgatt cccacagttg gaccgtacct gctaccgcat
    20641 attatcccta tgctgcacca gacctttcca aagctggaaa tgtatctgca tgaagcacag
    20701 acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat cctcgcgctg
    20761 gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat gttgctggct
    20821 atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac cgatggccga tctggcaggg
    20881 gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat gggtttctgt
    20941 tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga gacgctgcgc
    21001 aacatggtgg cggcaggtag cgggatcact ttactgcctg cgctggctgt gccgccggag
    21061 cgcaaacgcg atggggttgt ttatctgccg tgcattaagc cggaaccacg ccgcactatt
    21121 ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct ggcagaggcc
    21181 atccgcgcac gaatggatgg ccatttcgat aaagttttaa aacaggcggt ttaaaccgtt
    21241 taacgcagct acccgatagg cttccgccat cgtcgggtag ttaaaggtgg tgttgacgaa
    21301 gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga tatgaataat
    21361 ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt cccgatggaa
    21421 caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcgcgtgcca gatgtttaaa
    21481 ctgggcgcgg cccacttcat atggcacttt cattgcggtc agctgctgtt cggttttgcc
    21541 cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa tcagatgtgc
    21601 ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat aggccgctga
    21661 tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct gtgcggtctg
    21721 atacatgctg ttgaccttca gctgtccgcg gctgtcagtt tccagcccaa tgttctgtaa
    21781 cgccagcgaa tcggtattac cggtgcgacc gttggcatag agcaggcagt cagctttcag
    21841 ttttttaccc gacttcaaat gcatgatcac accatcgtca cagccttcga tcttctcgta
    21901 ctcttcgttg tgacgaatca ctacgccact gttccagaag tgataggaga gagaatctga
    21961 catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat ccacttttac
    22021 atccataccg cggaagatcg acgcatattc acagccgatc actccagcac catagataag
    22081 tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa tgcgtggatg
    22141 ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa caaatttttc
    22201 agcggttagt gtttcaacgc tgccgtccgg gcaatccagc gccaacgtat gctcgtcaac
    22261 aaagcgagcg tttccctgca atatttcaca gtgattacgt tcgtaaaatc cctgacgcat
    22321 gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa aagaagagcg
    22381 gagcagtcgg gaatggtcgc tgtaaagtgg gttttgattg aattctataa tgcggctgac
    22441 ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc cgccaacatt
    22501 ttgataacgc tcgataactg cgacgcgcgc accttgctta accaggccca ttgcagcgcc
    22561 ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat gtggcatggt
    22621 agggcttacc tgttcttata cataaaagca acagaatggt aacattttat cgcgggtaag
    22681 ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt tgtctgtttc
    22741 ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt ttaagcggca
    22801 ggtttccgct gtacgtaaaa gaaccggcca aagaattgca gtaaatatgt tttattgcgt
    22861 taccgttcat tcacaatact ggagcaatcc agtatgttca ttctctggta tagtgccagc
    22921 agtacttttg gcaaggattc agacatcgtg atgggcgtaa gagcgcaaca aaaagaaaaa
    22981 acccgccgtt cgctggtgga agccgcattt agccaattaa gtgctgaacg cagcttcacc
    23041 agcctgagtt tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc tttttatcgg
    23101 catttccgcg acgtagacga actgggtctg accatggttg atgagagcgg tttaatgcta
    23161 cgccaactca tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt gatccgcacc
    23221 tcggtctcca catttatgga gttcatcggt aataatccta acgccttccg gttattattg
    23281 cgggaacgct ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga aattcagcac
    23341 ttcattgcgg aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg tgcgtttact
    23401 gaagcgcaag ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga ggcgttggac
    23461 gtcggcgtcg aacaacgtcg gcaattagaa gagcgactgg tactgcaact gcgaatgatt
    23521 tcgaaagggg cttattactg gtatcgccgt gaacaagaga aaaccgcaat tattccggga
    23581 aatgtgaagg acgagtaatg aaacaagcaa atcaagatag aggtacgctg ctgctggcgt
    23641 tagttgctgg cttatcgatt aatggtactt tcgcagcgct gtttagctcc attgtgccat
    23701 tttctgtatt cccgattatt tccctggtgc tgacggttta ctgcctgcat caacgttatc
    23761 ttaatcgcac tatgccggta ggcttgccgg gtctggcagc tgcctgtttt attctcggcg
    23821 tactgctgta cagcacggta gttcgtgcgg aatatccgga tatcggctct aacttcttcc
    23881 cggcagtact ctccgtcatt atggtgttct ggattggcgc gaagatgcgt aaccgtaagc
    23941 aggaagttgc tgaataatcg gtattatgcc ggatgctggc gcatccagca tgggttttac
    24001 ttcgcggtca gtaatacgcc gcactccata tggtgcgtgt aggggaactg atcaaacaga
    24061 gccagacgtt cgaccttgtg cgtctggctt aatgtttcca gattcttgca taacgtttcc
    24121 gggttacagg agatgtacaa aatacacgga tacgcctgca ccattttctc ggtttcactg
    24181 tccagaccgc tgcgcggtgg gtcgacaaaa atggtttcgc actgataact ctttaagtcg
    24241 atcccttgca ggcggttaaa ctcgcgcaca ccattcatcg cctgagtaaa ttcttctgcc
    24301 gccatacgaa taatttgtac gttatcaata tggttagctg cgatgttgta ttgcgcagca
    24361 gcaaccgacg gcttagcgat ttcggtggct aatacccgat caaaattgcg cgccagcgct
    24421 aatgaaaagt taccgttgcc gcagtacagc tccagtaaat cgccttttga gcctttggtt
    24481 acgtccagcg cccattccag catctgaata ttcatcgccg cgttcggctg ggtaaagctg
    24541 ttttctacct gacggtagat catctctttc cctgcgaccg gcagacgctc gtcgatgtaa
    24601 tcttgatcca gctcgatttt ggttttcgtt gcccgaccaa tcagatgcac attcagattc
    24661 tgcgcgcgca gtgcatcgcg cagggcctcc gcttcctgac gccactcatc atccagcttc
    24721 ttatggtata gcagggaaac caccgcctga ttactcagcg tggtgaggta atcaatctgg
    24781 aacaacttgt ggcgcagaac gggattatta cgcacacccg caatcatcgc cgtcatcaac
    24841 tggttgataa gttcactggc ggcggggaag ctatccacgc ggatgcggct tttggtttgt
    24901 tgatcgaaaa tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa ctccgcgcgc
    24961 atccggtaat gactgaccgg cgagcgaaac acttccggaa ccaggtcaga aaacggtgcc
    25021 atcatacttt gcaaacgtac cactttttcg gctaactgcg cttcatactg ttctgttgga
    25081 aggtgttcgg gggtcatgat gtatcctgaa aaattaaagt acgcggcgat tgtagggatt
    25141 gctcatcaga tgtccagatc ttgatgaatt cctatttgtg agctacgtct ggacagtaac
    25201 ttgttacaac ctgtagcatc cacttgccgg tcctgtgagt taatagggaa tccagtgcga
    25261 atctggagct gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg cggacactgc
    25321 catccggtgg gaagtcatca tctcttagta tcttagatac ccctccaagc ccgaagacct
    25381 gccggccaac gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac ctgcggcatc
    25441 cttcttctat tgtggatgct ttacaatgat taaaaaagct tcgctgctga cggcgtgttc
    25501 tgtcacagcc ttttccgctt gggcacagga taccagcccg gatactctcg tcgttactgc
    25561 taaccgtttt gaacagccgc gcagcactgt gcttgcacca accaccgttg tgacccgtca
    25621 ggatatcgac cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc ttccgggcgt
    25681 cgatatcacc caaaacggcg gttcaggtca gctctcatct atttttattc gcggtacaaa
    25741 tgccagtcat gtgttggtgt taattgatgg cgtacgcctg aatctggcgg gggtgagtgg
    25801 ttctgccgac cttagccagt tccctattgc gcttgtccag cgtgttgaat atatccgtgg
    25861 gccacgctcc gccgtttatg gttccgatgc aataggcggg gtggtgaata tcatcacgac
    25921 gcgcgatgaa cccggaacgg aaatttcagc agggtgggga agcaatagtt atcagaacta
    25981 tgatgtctct acgcagcaac aactggggga taagacacgg gtaacgctgt tgggcgatta
    26041 tgcccatact catggttatg atgttgttgc ctatggtaat accggaacgc aagcgcagac
    26101 agataacgat ggttttttaa gtaaaacgct ttatggcgcg ctggagcata actttactga
    26161 tgcctggagc ggctttgtgc gcggctatgg ctatgataac cgtaccaatt atgacgcgta
    26221 ttattctccc ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa gttgggacgc
    26281 cgggctgcgc tataacggcg aactgattaa atcacaactc attaccagct atagccatag
    26341 caaagattac aactacgatc cccattatgg tcgttatgat tcgtcggcga cgctcgatga
    26401 gatgaagcaa tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg gtagtattgg
    26461 tgcgggtgtc gactggcaga aacagactac gacgccgggt acaggttatg ttgagaatgg
    26521 atatgatcaa cgtaataccg gcatctatct gaccgggctg caacaagtcg gcgattttac
    26581 ctttgaaggc gcagcacgca gtgacgataa ctcacagttt ggtcgtcatg gaacctggca
    26641 aaccagcgcc ggttgggaat tcatcgaagg ttatcgcttc attgcttcct acgggacatc
    26701 ttataaggca ccaaatctgg ggcaactgta tggcttctac ggaaatccga atctggaccc
    26761 ggagaaaagc aaacagtggg aaggcgcgtt tgaaggctta accgctgggg tgaactggcg
    26821 tatttccgga tatcgtaacg atgtcagtga cttgatcgat tatgatgatc acaccctgaa
    26881 atattacaac gaagggaaag cgcggattaa gggcgtcgag gcgaccgcca attttgatac
    26941 cggaccactg acgcatactg tgagttatga ttatgtcgat gcgcgcaatg cgattaccga
    27001 cacgccgttg ttacgccgtg ctaaacagca ggtgaaatac cagctcgact ggcagttgta
    27061 tgacttcgac tggggtatta cttatcagta tttaggcact cgctatgata aggattactc
    27121 atcttatcct tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc ttgcggttgc
    27181 gtatccggtc acctctcatc tgacagttcg tggtaaaata gccaacctgt tcgacaaaga
    27241 ttatgagaca gtctatggct accaaactgc aggacgggaa tacaccttgt ctggcagcta
    27301 caccttctga accacgtccc accgtgctgg tgtttgactc cggcgtcggt gggttgtcgg
    27361 tctatgacga gatccggcat ctcttaccgg atctccatta catttatgct ttcgataacg
    27421 tcgcttttcc gtatggcgaa aaaagcgaag agtttattgt tgagcgagtg gtggcaattg
    27481 tcaccgcagt gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc aacaccgcca
    27541 gtaccgtttc acttcctgca ttacgcgaaa agttcgactt cccggttgtt ggtgtcgtgc
    27601 cggcgattaa acctgctgca cgtctgacgg caaatggcat tgtcggatta ctggcaaccc
    27661 gcggaactgt taaacgttct tatactcatg agctgatcgc gcgtttcgct aatgaatgcc
    27721 agatagaaat gctgggctcg gcagagatgg ttgagttggc tgaagcgaag cttcatggcg
    27781 aagatgtttc tctggatgca ctaaaacgta tcctacgccc gtggttaaga atgaaagagc
    27841 cgccagatac cgttgtattg ggttgcaccc atttccctct actacaagaa gaactcttac
    27901 aggtgctgcc agagggaacc cggctggtgg attctggcgc agcgattgct cgcagaacgg
    27961 cctggctgtt agaacatgaa gctccggatg caaaatctgc cgatgcgaat attgcctttt
    28021 gtatggcaat gacgccagaa gctgaacaat tattgcccgt tttacagcgt tacggcttcg
    28081 aaacgctcga aaaactggca gttttaggct gatttggttg aatgttgcgc ggtcagaaaa
    28141 ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca
    28201 cggaacaacg gcaaacacgt cgccgggtca gcggggttct cctgagaatc tcaacagaga
    28261 aaagcaaaaa atgcttgact ctgtagcggg aaagcgtatt atgcacaccc cgcgacgctg
    28321 agaaaa
//
LOCUS       DAZGOB010000057        28283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGOB010000057 DAZGOB010000000
VERSION     DAZGOB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.057"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            14..88
                     /gene="tnaC"
                     /locus_tag="ACHLIF_002591"
     CDS             14..88
                     /gene="tnaC"
                     /locus_tag="ACHLIF_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO9606586.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            309..1724
                     /gene="tnaA"
                     /locus_tag="ACHLIF_002592"
     CDS             309..1724
                     /gene="tnaA"
                     /locus_tag="ACHLIF_002592"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO9606587.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMSLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1815..3062
                     /gene="tnaB"
                     /locus_tag="ACHLIF_002593"
     CDS             1815..3062
                     /gene="tnaB"
                     /locus_tag="ACHLIF_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO9606588.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3194..4369
                     /gene="mdtL"
                     /locus_tag="ACHLIF_002594"
     CDS             3194..4369
                     /gene="mdtL"
                     /locus_tag="ACHLIF_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO9606589.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4344..5303
                     /gene="yidZ"
                     /locus_tag="ACHLIF_002595"
     CDS             4344..5303
                     /gene="yidZ"
                     /locus_tag="ACHLIF_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO9606590.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5460..6209
                     /gene="yieE"
                     /locus_tag="ACHLIF_002596"
     CDS             5460..6209
                     /gene="yieE"
                     /locus_tag="ACHLIF_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO9606591.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            6231..6797
                     /gene="yieF"
                     /locus_tag="ACHLIF_002597"
                     /gene_synonym="chrR"
     CDS             6231..6797
                     /gene="yieF"
                     /locus_tag="ACHLIF_002597"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO9606592.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6851..8188)
                     /gene="adeP"
                     /locus_tag="ACHLIF_002598"
     CDS             complement(6851..8188)
                     /gene="adeP"
                     /locus_tag="ACHLIF_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO9606593.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8355..9020
                     /gene="yieH"
                     /locus_tag="ACHLIF_002599"
     CDS             8355..9020
                     /gene="yieH"
                     /locus_tag="ACHLIF_002599"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO9606594.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9087..9560
                     /locus_tag="ACHLIF_002600"
     CDS             9087..9560
                     /locus_tag="ACHLIF_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606595.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9609..10196
                     /gene="cbrC"
                     /locus_tag="ACHLIF_002601"
     CDS             9609..10196
                     /gene="cbrC"
                     /locus_tag="ACHLIF_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO9606596.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10258..10980)
                     /gene="yieK"
                     /locus_tag="ACHLIF_002602"
     CDS             complement(10258..10980)
                     /gene="yieK"
                     /locus_tag="ACHLIF_002602"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO9606597.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALD"
     gene            complement(10995..12164)
                     /gene="yieL"
                     /locus_tag="ACHLIF_002603"
     CDS             complement(10995..12164)
                     /gene="yieL"
                     /locus_tag="ACHLIF_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9606598.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12191..13807)
                     /gene="bglH"
                     /locus_tag="ACHLIF_002604"
     CDS             complement(12191..13807)
                     /gene="bglH"
                     /locus_tag="ACHLIF_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO9606599.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13893..15287)
                     /gene="bglB"
                     /locus_tag="ACHLIF_002605"
     CDS             complement(13893..15287)
                     /gene="bglB"
                     /locus_tag="ACHLIF_002605"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO9606600.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15306..17183)
                     /gene="bglF"
                     /locus_tag="ACHLIF_002606"
     CDS             complement(15306..17183)
                     /gene="bglF"
                     /locus_tag="ACHLIF_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO9606601.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17317..18153)
                     /gene="bglG"
                     /locus_tag="ACHLIF_002607"
     CDS             complement(17317..18153)
                     /gene="bglG"
                     /locus_tag="ACHLIF_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO9606602.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(18439..19164)
                     /gene="phoU"
                     /locus_tag="ACHLIF_002608"
     CDS             complement(18439..19164)
                     /gene="phoU"
                     /locus_tag="ACHLIF_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO9606603.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(19179..19952)
                     /gene="pstB"
                     /locus_tag="ACHLIF_002609"
     CDS             complement(19179..19952)
                     /gene="pstB"
                     /locus_tag="ACHLIF_002609"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO9606604.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(20043..20933)
                     /gene="pstA"
                     /locus_tag="ACHLIF_002610"
     CDS             complement(20043..20933)
                     /gene="pstA"
                     /locus_tag="ACHLIF_002610"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO9606605.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20933..21892)
                     /gene="pstC"
                     /locus_tag="ACHLIF_002611"
     CDS             complement(20933..21892)
                     /gene="pstC"
                     /locus_tag="ACHLIF_002611"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO9606606.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(21979..23019)
                     /gene="pstS"
                     /locus_tag="ACHLIF_002612"
     CDS             complement(21979..23019)
                     /gene="pstS"
                     /locus_tag="ACHLIF_002612"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO9606607.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(23267..24340)
                     /gene="lpfD-O113"
                     /locus_tag="ACHLIF_002613"
     CDS             complement(23267..24340)
                     /gene="lpfD-O113"
                     /locus_tag="ACHLIF_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO9606608.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPSPGQFTATATITVEIVR"
     gene            complement(24351..26873)
                     /gene="lpfC-O113"
                     /locus_tag="ACHLIF_002614"
     CDS             complement(24351..26873)
                     /gene="lpfC-O113"
                     /locus_tag="ACHLIF_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO9606609.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVST
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(26898..27629)
                     /gene="lpfB-O113"
                     /locus_tag="ACHLIF_002615"
     CDS             complement(26898..27629)
                     /gene="lpfB-O113"
                     /locus_tag="ACHLIF_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO9606610.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(27677..28249)
                     /gene="lpfA-O113"
                     /locus_tag="ACHLIF_002616"
     CDS             complement(27677..28249)
                     /gene="lpfA-O113"
                     /locus_tag="ACHLIF_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO9606611.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         7219 a         7094 c         6599 g         7371 t
ORIGIN      
        1 ggataagtgc attatgaata tcttacatat atgtgtgacc tcaaaatggt tcaatatcga
       61 caataagatt gtcgatcacc gcccttaatt tacccttctg tagccatcac cagagccaaa
      121 ccgattagat tcaatgtgaa cgattagttt gctatatctt aattttgcct tttgcaaagg
      181 ccatctctcg tttatttact tattttagta aatgatggtg cttgtatata tacatggcga
      241 attaatggat attgcagatg taatattcac agggatcact gtaattaaaa taaatgaagg
      301 attatgtaat ggaaaacttt aaacatctcc ctgaaccgtt ccgcattcgt gttattgagc
      361 cagtaaaacg taccactcgc gcttatcgtg aagaggcaat tattaaatcc ggtatgaacc
      421 cattcctgct ggatagcgaa gatgtgttta tcgatttact gaccgacagc ggcaccgggg
      481 cggtgacgca gagtatgcag gccgcgatga tgcgcggcga cgaagcctac agcggcagcc
      541 gcagctacta tgcgttagcc gagtcagtga aaaatatctt tggttatcaa tacaccattc
      601 cgactcacca gggccgtggc gcagagcaaa tttatattcc ggtactgatt aaaaaacgcg
      661 agcaggaaaa aggcctggat cgcagcaaaa tggtggcatt ctctaactat ttctttgata
      721 ccacgcaggg ccatagccag attaacggct gtaccgtgcg taacgtctat atcaaagaag
      781 ccttcgatac tggcgtgcgt tacgacttta aaggtaactt tgaccttgag ggattagaac
      841 gcggtattga agaagttggc ccgaataacg tgccgtatat cgttgcaacc atcaccagta
      901 actctgcagg tggtcagccg gtttcactgg caaacttaaa agcgatgtac agcatcgcga
      961 agaaatacga tattccggtg gtaatggact ccgcacgctt tgctgaaaac gcctatttca
     1021 tcaagcagcg tgaagcagaa tacaaagact ggaccatcga gcagatcacc cgcgaaacct
     1081 acaaatatgc cgatatgctg gcgatgtccg ccaagaaaga tgcgatggta ccgatgggcg
     1141 gcttgctgtg catgaaagac gacagcttct ttgatgtgta caccgagtgc agaacccttt
     1201 gcgtggtgca ggaagggttc ccgacatatg gcggcctgga aggcggcgcg atggagcgtc
     1261 tggcggtagg tctgtatgac ggcatgagtc tcgactggct ggcttatcgt atcgcgcagg
     1321 tacagtatct ggtcgatggt ctggaagaga ttggcgttgt ctgccagcag gcgggcggtc
     1381 acgcggcatt cgttgatgcc ggtaaactgc tgccgcatat cccggcagac cagttcccgg
     1441 cacaggcgct ggcgtgcgag ctgtataaag tcgccggtat ccgtgcggta gaaattggct
     1501 ctttcctgtt aggccgcgat ccgaaaaccg gtaaacaact gccatgcccg gctgaactgc
     1561 tgcgtttaac cattccgcgc gcaacatata ctcaaacaca tatggacttc attattgaag
     1621 cctttaaaca tgtgaaagag aacgcggcga atattaaagg attaaccttt acctacgaac
     1681 caaaagtatt gcgtcacttc accgcaaaac tgaaagaagt ttaattaata ctacagagtg
     1741 gctataagga tgttagccac tctcttaccc tacatcctca ataacaaaaa tagccttcct
     1801 ctaaaggtgg catcatgact gatcaagctg aaaaaaagca ctctgcattt tggggtgtta
     1861 tggttatagc aggtacagta attggtggag gtatgtttgc tttacctgtt gatcttgccg
     1921 gtgcctggtt tttctggggt gcctttatcc ttatcattgc ctggttttca atgcttcatt
     1981 ccgggttatt gttattagaa gcaaatttaa attatcctgt cggctccagt tttaacacca
     2041 tcaccaaaga tttaatcggt aacacctgga acattatcag cggtattacc gttgccttcg
     2101 ttctctatat cctcacttat gcctatatct ctgctaatgg tgcgatcatt agtgaaacga
     2161 tatcaatgaa tttgggctat cacgctaatc cacgtattgt cgggatctgc acagccattt
     2221 tcgttgccag cgtattgtgg ataagttcgt tagccgccag tcgcattacc tcattgttcc
     2281 tcgggctgaa gattatctcc tttgtgatcg tgtttggttc tttcttcttc caggtcgatt
     2341 actccatcct gcgcgatgcc accagcacca ctgcgggaac gtcttacttc ccgtatatct
     2401 ttatggcttt gccggtgtgt ctggcgtcat ttggtttcca cggcaatatt cccagcctga
     2461 ttatttgcta tggaaaacgc aaagataagt taatcaaaag cgtggtattt ggttcgctgc
     2521 tggcgctggt gatttatctc ttctggctct attgcacgat ggggaatatt ccgcgcgaaa
     2581 gctttaaggc gataatctcc tcaggcggca acgttgattc gctggtgaaa tcgttcctcg
     2641 gcaccaaaca gcacggcatt atcgagtttt gcctgctggt gttctctaac ttagctgttg
     2701 ccagttcgtt ctttggtgtc acgctggggt tgttcgatta tctggcggac ctgtttaaga
     2761 ttgataactc ccacggcggg cgtttcaaaa ccgtgctgtt aaccttcctg ccacctgcgt
     2821 tgttgtatct gatcttcccg aacggcttta tttacgggat cggcggtgcc gggctgtgcg
     2881 ccaccatctg ggcggtcatt attcccgcag tgcttgcaat caaagctcgc aagaagtttc
     2941 ccaatcagat gttcacggtc tggggcggca atcttattcc ggcgattgtc attctctttg
     3001 gtataaccgt gattttgtgc tggttcggca acgtctttaa cgtgttacct aaatttggct
     3061 aaatccttca agaagccagc cattcgctgg cttcttgcct ctcaggaaat cacttatgtc
     3121 caaatggcaa ctcgcctgat tctccttcac cacgtatgct ttgcgtcacc ttactatcag
     3181 gacgctttag cccatgtccc gctttttaat ttgtagtttt gccctggttt tactttatcc
     3241 cgccgggatt gatatgtacc tcgttggttt accgcgcatc gccgccgatc tcaatgccag
     3301 cgaagcgcag ttgcatattg cgttctccgt ttatctggcg ggcatggcag ctgcgatgtt
     3361 atttgccggt aaagtggccg atcgttcagg gagaaagcct gtcgccatcc ccggcgcggc
     3421 gctatttatt atcgcttcgg tgttctgttc actggctgaa accagcacgt tatttcttgc
     3481 agggcgcttt ctacaggggt tgggcgcagg ctgttgttac gtggtggcgt tcgccatttt
     3541 gcgcgacacg ctggatgatc gacgtcgggc taaagtgctg tcgttactca acggtattac
     3601 ctgcatcatt ccggtgttag cgccggtgct cggacacctg attatgctta aattcccttg
     3661 gcagagtctg ttctggacga tggcaatgat gggcatcgcg gtactgatgt tatctttgtt
     3721 tattttaaaa gaaacgcgcc cagcgtcccc cgctgcttcg gacaaaccac gagaaaatag
     3781 cgagtcgctg cttaatcggt ttttcctcag ccgtgttgtt atcaccaccc tcagcgtttc
     3841 ggtgatcctc actttcgtca atacatcgcc ggtattgctg atggaaatca tgggttttga
     3901 gcgcggagaa tacgccacca ttatggcgtt gaccgctggc gtcagcatga ccgtttcatt
     3961 ctccacgcca tttgcgctgg gaatttttaa gccacgtacg ttgatgatca cctcgcaggt
     4021 gttattcctt gcggcgggga tcacccttgc cgtttcacct tcccatgcgg tttctctgtt
     4081 tggtatcacg ctgatttgcg ccggtttctc ggtaggtttt ggcgtagcga tgagtcaggc
     4141 gttaggacca ttttcattac gcgcgggcgt agccagctcg accttaggta ttgcgcaggt
     4201 ttgcggttcg tcactgtgga tttggctggc agcggtggtt ggtatcggcg catggaatat
     4261 gctgatcggg attctgattg cctgtagcat agtgagcctg ttactgatta tgttcgtcgc
     4321 gcctggacgc cccgttgccg ctcatgaaga aatccatcac cacgcttgat ctcaatctgc
     4381 tgctctgtct gcaactgctg atgcaggagc gcagcgtgac caaagcggcg aagcggatga
     4441 acgtgacccc ttcagcggtg agtaagtcgc tggcaaagtt aagagcgtgg tttgacgacc
     4501 cgctctttgt gaactcaccg ctggggctgt cgcccacacc gctaatggtc agcatggagc
     4561 aaaatctggc ggaatggatg caaatgagca atctgctgct ggataaaccg caccaccaga
     4621 caccgcgcgg cctgaagttt gagttggcgg cggaatcgcc gctgatgatg atcatgctta
     4681 atgcgctgtc gaaacggatc taccaacgtt acccgcaggc gaccatcaaa ctacgtaact
     4741 gggattacga ttccttagat gccattactc gtggtgaagt ggatattggt ttttccggtc
     4801 gcgaaagcca tcctcgctcg cgggagctgt taagctcgct accgttagcc attgattatg
     4861 aagtgctgtt tagtgatgta ccctgcgtct ggttacgcaa agatcatccg gcactgcatg
     4921 aaacgtggaa tctggacacc tttttacgtt atccgcatat cagcatttgc tgggaacaga
     4981 gcgacacctg ggcgctggac aatgtgttac aggagctggg gcgcgaacgc acgattgcta
     5041 tgagcctgcc ggaatttgag cagtcactgt ttatggcagc gcaacccgac aatctgctac
     5101 tggcgaccgc gccgcgctac tgtcagtact acaatcaact ccatcaactg ccgttggttg
     5161 ctcttcctct cccgtttgac gaaagccagc aaaaaaagct ggaagttcct tttaccctgc
     5221 tgtggcataa acggaacagc cataatccga agatcgtctg gttacgggaa accattaaaa
     5281 acctttacgc gtcgatggca taaccgaatc gtatgaaacg cgacccaatt tcacaataaa
     5341 atgtaaaaaa gttgtaataa gcttgtctga atcgaacttt tagccgcttt agtctgtcca
     5401 tcattccagt aaatgattac tcttatattc ataatggacc attaagcatg gagcgaaaaa
     5461 tggcgactca ctttgcccga gggattttaa cggaaggaca tctgatttct gttcgtctcc
     5521 cctcccagtg tcatcaagaa gcccgaaaca ttccccctca tcgtcaaagc cgttttctgg
     5581 cgtccagagg tttactcgca gaactgatgt tcatgctgta tggcattggc gaattgccgg
     5641 aaatcgtcac cctgccgaaa ggtaaaccgg ttttcagtga taaaaatttg ccttcgtttt
     5701 ctatttccta tgccgggaat atggttggcg tggcgttaac aaccgaaggt gaatgtggcc
     5761 tcgatatgga actacagcgt gcgacgcgcg ggtttcatag cccacacgcg cccgataacc
     5821 acaccttttc cagcaatgaa tcgctatgga tcagtaaaca aaacgatcct aacgaagcgc
     5881 gggcgcagct catcacgctg cgccgaagcg tgctaaaact aaccggtgat gttttgaatg
     5941 acgatccgcg cgatctgcag ctgctgccca ttgccggacg cctgaaatgt gctcatgtaa
     6001 atcatgtaga agcgttatgc gacgcggagg acgtgctagt gtggtccgtg gcggtcacgc
     6061 ccgcgattga aaagctcagt gtctgggagt tagatggcaa acacagctgg aaaagcctgc
     6121 cggatattca cagccgcgcc aacaatccta ccagccggat gatgcgtttt gcccagctct
     6181 ctaccgtgaa gtctttttcg ccaaattgat agacaaccac aggagtcatt atgtctgaaa
     6241 aattgcaggt ggttacgtta ctggggagcc tgcgcaaagg ctcatttaat ggcatggttg
     6301 cacgtaccct gccgaaaatt gctccggcga gcatggaagt caatgcgtta ccttccattg
     6361 ccgacattcc cctgtatgac gctgacgtac agcaggaaga aggttttcca gcaacggttg
     6421 aagctctggc ggaacagatc cgtcaggctg acggtgtggt gatcgtcacg ccggaatata
     6481 actactcggt accgggtggg ctgaaaaatg ccatcgactg gctttcccgc ctgccggatc
     6541 aaccgctggc cggtaaaccg gtgttgattc agaccagctc aatgggcgtg attggcggcg
     6601 cgcgctgtca gtatcacctg cgccagattc tggttttcct cgatgcaatg gtgatgaaca
     6661 agccggaatt tatgggcggc gtgattcaga acaaagttga tccgcaaacc ggagaagtga
     6721 ttgatcaggg tacgctggac cacctgaccg ggcaattgac cgcatttggt gagtttattc
     6781 agcgagttaa gatctaaata aaaaacccgc cagcaatcat gcatggcggg tttttaacgc
     6841 gctatcgatt ttagtgagcg tcgataaaga caatcttcag gataaacagc agcgcaacga
     6901 tgattacgca cgggcttaga tcacgcagac gtccggtacc gattttcatc acgcagtagg
     6961 agataaagcc cagcgcgata ccttcggtaa tcgagaagct gaacggcatc atcacggcgg
     7021 taataaacgc cggaacagat tcagtaagat cctgccagtt cacgcgtgcc agactggaag
     7081 tcatcagcac gccaacgtaa atcagcgcac cagctgcagc gtagcctggc accatccccg
     7141 ccagcggcga cagaaagata accagcagga acagcagacc aacaaccact gccgtcagac
     7201 cggtacgacc gccaaccgat acgccggaag aggactcaat ataagccgta acggaagaag
     7261 taccgataaa cgaaccggtc acggaagaga tactgtcgac atacagcgcc tgcttcatgc
     7321 gcgggaattt acccttctca tccgccaggc ctgctttatc ggtcacgcca atcagcgtac
     7381 cggaggagtc aaacaggttg accagcatga aagagaaaat cacccctgcc agcccgaggt
     7441 taaacgaccc ggctaaatct acatgaccca caactgtcat tacgctcggc ggcgcagaaa
     7501 cgatgccatt gtagtgcaca tcacccagca tccagcccag cagcgtcgtc accacgatag
     7561 aaaccagcac cgctgcgtga atgttgcgcg aggccagaat agcaatgatg aagaagccga
     7621 ggatacccag aagtacgctg tgagaagtca gattaccgat gctcaccagc gtttccgggt
     7681 tagcgacaat cacacctgcg tttttcagcc ccatcatgcc aatgaacaga ccgataccgc
     7741 tggtaatacc cacacgcaga ctcaccggaa tgttggcaat catccagtag cgaacgcgga
     7801 aaatcgtcag taacagcaga cctatcgcgc cccagaagat tgcgcccatc ccgacctgcc
     7861 acggcaagcc catcgcctgt acaacgacaa aagcgaagaa cgcattcagg cccatagcgg
     7921 gtgccagtgc aactggcagg ttagcaaaca gtcccatcat aatactgccg aatgcagcga
     7981 tcagacaggt agtgacgaag acggcgctgg tatccatgcc agcaacgcca agaatttgcg
     8041 ggttaacaaa aacgatgtaa accatcgtca ggaaggtggt aaaaccggcg atcacttcgg
     8101 tccgtgccgt cgtgccatgt tcgcgcagtt taaacacgcg ttccagcatc ccctggccag
     8161 aagcctgggt tgtgtgttgt tgactcatta tctatttccg aacaaggagg gaaaatccgt
     8221 cgctatcgta taccaaaatg agacaatatg gacggctgaa gataactttt tttcattgta
     8281 tttgcttata cggcaacgat tgcgtcatgc gaattggcat gacgaaaacg ttaaactaac
     8341 gataaaggaa agacatgtcc cagatagaag cggtattttt cgactgcgac ggtacgctgg
     8401 tcgacagtga agtcatttgc tcccgcgcat atgtaacgat gtttcaggaa tttggtatta
     8461 cgctcgatcc tgaagaggta ttcaaacgtt tcaaaggtgt aaaactgtac gaaattatcg
     8521 atattgtttc ccttgaacat ggtgttacgt tagcgaaaac agaagctgaa cacgtttacc
     8581 gtgcagaagt cgctcggctg ttcgattcag aactggaagc catcgaaggg gctggagcgc
     8641 tcctgtcagc gatcactgcg ccaatgtgtg tggtatctaa cggcccaaat aacaaaatgc
     8701 agcattctat gggcaagctg aatatgttgc actacttccc ggataaactg ttcagcggct
     8761 acgatattca gcgctggaag ccagacccgg cgttaatgtt ccatgcggca aaagcgatga
     8821 atgtaaatgt agaaaactgc attctggttg atgactcagt tgccggtgcg caatctggta
     8881 tcgacgcagg tatggaagtg ttctacttct gcgccgaccc gcacaataag ccgatcgttc
     8941 acccgaaagt caccaccttt acccatcttt cgcagttacc ggaactgtgg aaagcgcgtg
     9001 gttgggatat tacggcatag ttcttcacac tcccttcact taccccgctt aaattggcgc
     9061 tcaaaggtaa gtaaagggag tttgatatgt ctgtttcacg tcgggtaata catcacggac
     9121 tttattttgc ggttttagga ccgttaattg gtgttctgtt tcttgtcctc tacatattct
     9181 tcgcaaaaga accgctggtt cttttgcgaa gaatacaggt acttccattg tttctcttat
     9241 tgtcgattac tacgggagtt attcctgcat tgttaaccgg cgtgatggtc gcctgtctgc
     9301 cggaaaagat cgggtcacag aaaaattatc gttgtctggc tggtggcata ggtggcgtcg
     9361 ttatcaccga gatctattgt gcagttattg tacatattaa gggcatggct tcctcggagt
     9421 tgtatgaaaa cattctttct ggtgacagtc tcgttgtccg catcattcct gcattgctgg
     9481 caggtgtggt gatgagcaga atcattaccc gtctgcccgg attggatatt tcatgtcctg
     9541 aaacagactc tttaagttaa gcgggatact ttatctttgg gctactcaaa agcagacagg
     9601 atgttactat gactcaaaat atcaggccgt taccccaatt caaatatcat cccaagccac
     9661 tggaaacagg cgcatttgaa caggataaaa ccgtagagtg cgattgctgt gaacaacaga
     9721 cgtcagttta ttactcgggt cccttttatt gcgttgatga agttgaacat ctctgtccgt
     9781 ggtgtattgc ggacggttct gctgctgaaa agtttgcagg tagttttcag gatgatgcca
     9841 gcatagaagg tgttgaattt gagtatgatg aagaggacga atttgccggt attaagaaca
     9901 catatcctga tgaaatgctg aaagagttgg ttgaacgcac gccaggttat catggatggc
     9961 aacaggaatt ctggcttgcg cattgtggcg atttctgtgc ttttatcggc tatgtgggct
    10021 ggaatgatat aaaagatcgc ctcgatgaat ttgccaacct tgaagaagat tgtgagaatt
    10081 tcggtattag aaattctgat ctagctaaat gcctgcaaaa gggtggtgat tgtcagggtt
    10141 atctcttccg ctgtctccac tgcggcaagc tgagactgtg gggtgatttt tcgtagttat
    10201 ttaaataatg agaacaggcc ggagcgtaat tcatacatcc ggccttattt cttcagctta
    10261 atcgagcgct aattctgcgg cagcggcttt atcagcgatt accatcagcg aaggatgcag
    10321 ctgcaacacg gaagctggaa catcttcggt aaccggacct tgcagcacat ttttcagtgc
    10381 ctgcgcttta cctgcgccac tgacaattat cagcaagttt ttcgccgcca tgatgctttt
    10441 cggccccatc gtcacgtagc tgtcaggcac taacgaaaag tcgccgccca actcaccatg
    10501 cgccacaata tcaaccatct ctccctggat cgggaactcc acggtttgct catggaaatg
    10561 ggtcgtattc ggcaaattgc cacaaaaatg accatctgca cctaatccca gcaccaccaa
    10621 atccaggccg ccttcacgcg ccagtttctg atcatgctcg cggtagttat caatagtgag
    10681 cttctggata ttctcttctt tgatccccgc aggggtgaaa aacagattac gcagattggt
    10741 aatcgttaca ccttctccct ctttgccgcg aaagggaatt tcatcgaaat tatagaaata
    10801 gcagttatca taccagggct tacctttaac cagggtggtg aggtattcat acatgccttt
    10861 gggcgtgcta ccggcggtaa ttgccaggtt aacacgacgc gtcttcgaca tataaccaag
    10921 cagatggtgt gccgcgacac ggctcatttc ctggtaatct tcggtaatga ttaatttcat
    10981 cttagtgcct tattttattt aaataatttc tgtacgaagg ctgcataggc aggccgccag
    11041 acatccattt catggttcag gccgggatat tcctggtaat caaacttaat ttttttctgc
    11101 tcaagctcag ttttcagccc ggcgatatcc ttgccggtta cgacatcttt atcaccaacg
    11161 accacagtaa aattacgtag ttgctggtta atagctgccg gatcgttcag ccgggccgcg
    11221 acaccttcat ccggtacggt tgtcgtggta acaccactga atgtggccag ccagccaaag
    11281 ctttccagat gattcattcc ggaaaccagc gcctggtacc cgccttgtga aagccctgcc
    11341 agcgcgcgac catcggcatc tttacggaca ttaaaacgct tgctaatcag cgggataata
    11401 tcgttcatca gttcgcgatc tgccgcttta gcattcagcg gataaaagac tttacgtctt
    11461 tcctgaggca cgaaatcttc gggaataatg cccttcgcat cggtttcggt atccgggatc
    11521 accaccagca tcggtttaat tttcccttca gccagcaggt tatccatgat ttgcgggata
    11581 cgcccctgat cgatagcgga acgtccggta tcaccaaaac cgtgatagaa atagagcact
    11641 ggcaaaggct cgcccatgcc ggtgtaacct ggcggggtcc agacatacat ctgacgttca
    11701 gattgcaatg cgttggagtg gtaagttatg gcgatcaaat caccatgcgc aacagaacgc
    11761 gtatccagat aactgcctgg caccagaatc atactggagt taacctggcg ctgaggctta
    11821 gtcattgcgg tgcctgtatc aatgctgcgt acaccatcaa cattgaaaaa atactcgtac
    11881 aggttgcctt tcaggatagg tgtgcgccac gaccagactc ctgcttcgtc tttggtcatc
    11941 gggtgaatat tgtccggaac cggaacaccc accactacag agacattttt tgccccaggg
    12001 gcaaagtagc gaaaggtcac gctgttatcg gcattgacct gagtcacata ctgtttcacc
    12061 gggatagtgg gtgccgggct ggctggcata tccgctgcta tggcccactg agcagaaata
    12121 ccgctggcaa tagccagcgt taaagcagca atttttatat tcatcgtaat attcctttca
    12181 ttaagttcca ttaccaccag atttcagcct gggcaccgac agcgaactgg tcgtctttat
    12241 tgtcttcgaa ggtgaagcca tccagttcgt tttccagggc tttgatatac gtggcgtaga
    12301 aacgaatttc cggacgcgag gtcaacatac tggtattgac tttaaaggta tggaagagcg
    12361 tggttttata accagactca ttaaatttat tactattcgc atctttgttt tgctgggtga
    12421 aatagcccag ttcaacacct gtctggttat attggtccca aatataggcc gggcgaacaa
    12481 ccgcacgaat agattcgaaa tcagagtgtg cgcctgtttc gtagctataa atatcgttac
    12541 cgaaggagta aacaatcgcg ttagccacaa taaaatgatc gccgatatag gcttcgccct
    12601 gtgaagtcag acgaactgct gttccgccgg tgtgatcacc ataataacga ccattaaagg
    12661 tggtaaatgg acttgcgcca gcataacggc caaagttact ggcgatagag ttattcgcaa
    12721 ccaggaagga gaattcgttg aagccacctt tatcaaattt ctgcgttaaa gatgtgccaa
    12781 acatccaggt atctttccag tcataatagc cgttattatc ctgattatct ttttccgatg
    12841 cgctttcgtt tgccgtgaca taacgaccac tcaccattaa ggtcgcttta tcccataacg
    12901 ggatatcttt atagcgtaaa tcaatagtat tggtattaat ctgctgtttg ttttgcaggc
    12961 tgcgatcgta atcatcgaca tcttcgcgaa ccagcgcgat atcaatttta cccggaccga
    13021 ctttccagtt ttccagacct acacccgctg cggcatcagt acgctgcgtt ttccagtcca
    13081 gcatctggat ttcaattttc ggcgcaccgt gtttacccac ccagaaatca gcctctggcg
    13141 caaagggcag gaaacctttg gtggtaacgt acatatcgga gaactgcata aagttctcgc
    13201 caccggcgtt atcgccaaac cagccggtag agtactgctg accaacgtta ccatccatca
    13261 tcacaacggc atcaacccgt ttgccgtttt cgttgtagac acgttgtttt aactgcaaat
    13321 caaaccagcc ggagtattcg ttaccaaagc ggcccagaga accaatcgcc catgatttag
    13381 gtgaaccatg agaggcggtc ccccaaccag aacggtaata gccgttgtaa ctaaagccaa
    13441 tttcatcttt cacaaactta ctgaaatctt tcagagtcat ggaagaataa atcgagcctg
    13501 tctcactggc atttttctct tcatttttga ccagtacagc atcaggtttt gcggcactac
    13561 tggtcgggaa cggattggcg gcataccctt gatcattcgt acttacgcta cgattcaccg
    13621 tcgctggcgt atattttttc ttctcttcat ctttatactt tttgagttcg ctttgcgttt
    13681 ctcttaacgc tttttctaat aactcaaggc gatgttccac cgttaatgat tcagccaatg
    13741 attgtgcaga aaaggctaac ggtgccatta ataagatggc agaggtaata agatttcgtc
    13801 taaacataat atccctttat ggtgcaaaga aagaattaac gtatcgcatc cagactgttc
    13861 tgaatgcgac gataattaag gtgctttatt ggttattttt ttaatgacag cccccgcgtt
    13921 ttgatcactt ctgcatacca tccgaagctt ttcttacgcg ttcttgtgag gcttccttcg
    13981 ccattatcat cacgatccac ataaataaag ccgtagcgct tagacatttg tgaatgagat
    14041 gcactgacta aatcaattgg cccccaactg gtgtacccca taatatccac accatcggca
    14101 atcgcttcat ttacctgtac caggtgatcg tttaaatagg caattcgata atcgtcctgt
    14161 atcgaaccat ccgcttcaac gctgtctttt gcgcctaatc cgttctcgac aataaataac
    14221 ggtttttgat aacgatccca aagcgtattt aacagaaccc gtaatccaac cgaatcaatt
    14281 tgccaccccc actctgaact tttcagatgc ggattgggga tcatattcag tatgttgccc
    14341 tgcgcatttt tattaatgct ttcgtcgtgg gaaacacaac cagtcatgta ataactaaaa
    14401 gagatgaaat cgacggtatg ttttaaatct tctgcgtcac tttcagtcat ctcaatggtg
    14461 atattgtggt cgcggaagaa acgctgcata tagccgggat actggccacg cgcctgaaca
    14521 tcaccaaaga acatccagcg ccggttctct tccatggcct gcaacatatc ctgtggctgg
    14581 caggtgaggg ggtaaaccag cccaccgaga agcatattgc cgattttcgc ttcggggagc
    14641 aggctatgac aggctttaac tgcccgcgca ctggcaacca gttgatggtg gatagcctga
    14701 taaacttccg cctcgccact ctcttctgcc agccccacgc ccgtgaatgg ctcgtgtaac
    14761 gacatgttga tttcattaaa cgtcagccat aacgccactt tatgttggta gcgagtaaag
    14821 accgtgcggg cgtaatgctc gaagtgatcg atgaccgctc gattagccca accgccgtag
    14881 tttttcacca gcccatatgg catttcgtaa tgggataacg ttaccagcgg cttgatcccc
    14941 gcctgcgcca tttcatcaaa cagccgatcg taaaacgcta accccgcttc attcggttcg
    15001 acttcgtcgc cctgagggaa aattcgcgcc caggcaatgg aaatacgcag acaggtgaag
    15061 cccatctcgg caaataacgc gatatcttcc gggtaacggt gataaaaatc gatggcgaca
    15121 tctttgatat tctctttccc caggatgcgc ggttccattt ttcccattac gccatgaggc
    15181 tgtaaatctg aggtcgagat ccctttgcca tcttcctgcc aggcaccttc cacctgattg
    15241 gcagctgttg cgccacccca aagaaatgtt tctggaaatg ctttcataat taactccttt
    15301 tatcgttagc gaatgatgga taacagcggt tcacctgcgc ttatctgcgc cgtgccgtgg
    15361 ggtaatacgt ccgtaaaatc atcgctatta ctgattaata ccggcgtcgt cagatcaaat
    15421 ccggcctcgc gaatagcagg gatatcaaaa gaaatcagcc gatcgcctgt attgaccttg
    15481 tcacccacgt tgacgtgagc ggaaaagaat ttgccgtcca gttttacggt gtcgataccg
    15541 acatgaatca ggagctccac accatcatct gactcaatgc caatggcgtg taatgtggcg
    15601 aacaacgaag caattcgacc cgcaaccgga gaacgcactt caccaaccga gggcagaatg
    15661 gcgatacctt tacccaacag gccactggca aacgtggtat cagcgacgtg aatgagcggc
    15721 acaatctctc ccgtcatcgg tgaacagata ccgccctgct caggtggtgt aataacctct
    15781 ggtgttttct cttgcggggc accctgcgct ggctgacgtt tagcggtgat gaaatgaagc
    15841 atcaccgtac cgacaaatgc gcaaccgatg gcaatgacac cgccaataac gctggcccag
    15901 acggtgaaat caattcccgt tgacgggatg gtttgcatga aggtgaaaat acttggcaaa
    15961 ccaaaggagt acactttcgt ttgcgcgtag ccaatgatgg tggcccccaa agccccactg
    16021 atacaggcga taacaaaggg gtacttacgc ggcagattaa cgccatatac cgctggttcg
    16081 gtgataccaa acaggcccgt caacgccgct gatcccgcca ccactttttt ctgcgcatcg
    16141 cgttcgcaga ggaagacgcc gagcgccgcc ccgacctgcg ccataatggc gggcattaac
    16201 agcgggatca tggtgtcgta gcccagcacg gtgaagttat tgatacacag cggcaccagg
    16261 ccccagtgca gtccgaacat gacgaagatt tgccagaagc cgcccattac cgcgcctgca
    16321 aatgcaggaa ccgcctgata aagccagaga taaccggcgg caatcagttc gcttatccag
    16381 gttgatagcg gccccaccag cagaaaggtg acgggtgtga taaccatcag acatagcaat
    16441 ggtgtgaaga aatttttgat tgccgacggt aaccacgcat taagtcggcg ttccagaatg
    16501 ctgcacaacc aggcagaaaa aataatggga ataaccgatg acgagtaatt caacaatgtg
    16561 accggaatac ccaggaaatc cagccccagc tcatccgctt tttgcccgtt ctcgaaagca
    16621 gtcagaatta atggatgcac taacgctcca ccaatcacca tggcagtaaa tggattaccg
    16681 ccgaagcgtt tccccgcggt gtatcccagg attatcggga agaaccaaaa caaggcatca
    16741 ctggcgctga ataaaattaa ataagtacca ctttgttcgg tcgtccactg aaaagtgagc
    16801 gccagagcca gcataccttt caggatcccg gttgccgcca tcaaaccgat cagaggcgta
    16861 aaaatacctg aaataacata aacaaagcgg tttagcagat tacctttctc atcattttcc
    16921 ggtgcctgtt gcgctttttc gccaaggcct gccacgctgt tgaccgccag gaagacatcg
    16981 gccacatggt tacctatgac cacctgaaac tgaccaccgc tttccaccac cataataata
    17041 ccgggggtct ttttcagtac ctcagcttgc gctttgcttt catcctttaa cttaaaacgt
    17101 aatcgcgttg cgcaatgcat cagactcaca atgttatctg cgcccccgac tcctgcgact
    17161 atttttctgg ctaactccgt cataacttgc cctctaccgc tttgcggcaa aactccaaaa
    17221 aaaaacctga aaaaaacggc ctgacgtgaa tcaagcaatt tttttcaggt tttgcccgct
    17281 tagtgcggta acaatccttt actcagtaat aatatttcag tgttctttgc gcacgcgctc
    17341 tatatttatg gctaaaaaca taatctctgc gggtgaaatt ttacgttgat actgcaaacc
    17401 aataaaaatg gcgatccgtt ccgcacattg ccatgcttgc gggtaatttt gttttactgc
    17461 ttgttgtaat gattcatcac tatcgttaat tgaagcatgt tcaagaatac gccaggataa
    17521 aaacttcaga tgtgtaacca gtcgctgata actcaagctt tcttcctggt aattaaggct
    17581 gaactgaaat tttattaatt gcagcatttc gcgcattaac tgcgtgacac ctgcaacatc
    17641 ctccatattt ccgctcattt gggcactgac cagatgcatg gcaataaagc ccacttcatc
    17701 tttcggtaac tgcacgccca accgtttatc aataatggtt aacgcttctt cccctagctg
    17761 gaactctttc gggtaaagcc gctggatatc ccacagcaac gggttgggca gcaacacgtt
    17821 ttgctgaaag cgtttaatcg caaactggca atggtcagtt agcgagatat aaatactgtc
    17881 ctgtaatttt cccaagcgct cctgcgctaa agagataata cgatcacagg ttgccatcac
    17941 ctcaagagga atatgactta agagttcgct taatcgcccg ttcagttcat gactgctcaa
    18001 ggcatactct ttttctattc cacttgagtt aattctttcg ccagcgcgtt tttgaaagcc
    18061 aattcctcgc cccatgacga ctttttcccg ctgttgatca tcaataacca ccacaacatt
    18121 attgttgaga attttggtga tttgcatgtt catagcaagg acctttttta taaacaaaaa
    18181 aacccgactt caccagtatt ctctggttat gtcaggtttt gcctgcgaat gcagtaacaa
    18241 tccagtcatt tattaatggt ttttataacg aacatccagg ttcggaaatt aatttaattg
    18301 cgtgcagaga aaatagcaat gcgctattga taaaaatatg accatgctcg cagttattaa
    18361 ctttgtgtaa ttttaggaat ttataaagtt atatataaca aatcccaata attaagttat
    18421 tgggatttgt ctggtgaatt atttgtcgct atcttttccc gccagcagtt tatccagctc
    18481 atcaccgcct acatgacgga aatcctgccc cttcacgtag tagaagataa actcacaaat
    18541 attctggcag cggtcgccaa tacgttcgat agaacgcgcg cagaacagcg cagtaagtac
    18601 gctcggaatg gtacgcgaat cttccatcat gtaggtcatc agttgacgaa caataccttc
    18661 gtattcctga tcgacttttt tatcttcacg ataaatacgt accgcttcgt caatgtccat
    18721 ccgcgcgaac gcgtccagca cgtcgtgcag catctggatg gtatgacggc ccagcgactc
    18781 cagacttacc agcaacggct gatgctgctg ggagaatttc tccagcgcag tacggcagat
    18841 tttgtccgcc acgtcgccaa tacgctccag ctcggcaatg gttttactga tcaccataac
    18901 cagtcgcagg tcgctcgccg tcggctgacg tttggcgata atgcgcacgc aggcttcatc
    18961 gatcgccact tccatcatgt tgacgttctt gtcgccttcg atcacgcgct tcgccagatc
    19021 gctgtcctgg ttatgcatcg cggtgattgc atcagaaagc tgctgctcca ccatgccgcc
    19081 catggtcatc acctgcgtgc ggatactttc cagttcggcg ttgaactggc cggaaatatg
    19141 tttattaaga ttgagactgt ccataacgca ctcctgaatc aaccgtaacg accggtgatg
    19201 tagtcttctg tttgtttctt cgctggcttg gtgaacagat cgtccgtgtt gctgaactca
    19261 atcaattcgc ccaggtacat aaacgccgtg tggtcggaac aacgcgcagc ctgctgcatg
    19321 ttgtgggtga cgatcaccac ggtgtaatcc tgcttcagtt cggtgatcag ctcttcaata
    19381 cgcccggtag agatagggtc gagcgccgaa cacggttcgt cgagcagcag cacttccggg
    19441 cgaatggcga taccacgcgc aatacacaga cgctgttgct gaccaccaga gagagagtaa
    19501 ccgctctggt gcaatttatc tttggtttcg ttccacaatg cggctttggt caatgcccac
    19561 tgcacgcgct cgtccatgtc ggcacgggag agcttctcaa acagacgaac gccaaaagcg
    19621 atgttgtcgt agatggacat cggaaacggc gtcggtttct ggaacaccat gcccactttc
    19681 gcacgcagca gggcgatatc ctgagagttg gtcaggatgt tgtcgccatc aagcagaatt
    19741 tcaccttccg cacgctgctc cgggtacagt tcaaacattt tgttgaaggt acgcagcagc
    19801 gtcgatttac cgcagccgga cggcccgata aacgccgtta cctggttttt agcgatatcc
    19861 aggttgatgt ttttcagggc atggaatttg ccgtagtaga agttcaaatt acgaacctga
    19921 attttactcg gggcagtttc aaccatactc attgcaatct tttcctcatc gtgcctgatg
    19981 cacttcgcta atcaggctta ccgtaggccg gatcaggcgt tcacgccgca tccggcaaag
    20041 tgttaaccgt gtttattctt cgcaaaaaca acgcgcgcca gaatgttcag cagcagtacg
    20101 cacagggtaa tgatcaatac cccggcccag gccagttgct gccattccgc aaacggactc
    20161 atcgcaaact taaagatcgt caccggcagg ttggcgatcg gctgcatcat gtccgtgctc
    20221 cagaactggt tggagagcgc ggtaaacagc agcggcgcgg tttcaccagc aatacgggca
    20281 atcgccagca agataccggt catgatcccg gacaccgacg ctttcagcgt aatcgcggag
    20341 atcatcttcc acttcggtgt acccagcgca taagccgctt cacgcaggct gtccggcacc
    20401 agtttcagca tgttctcggt ggtgcggata acaatcggca cctgcaacag cgccagcgca
    20461 attacgcccg cccagccgga gaagtgctcc atctgtgcca ccacgatggt gtagacaaac
    20521 agaccaacca caatcgacgg cgcggagagc agaatgtcgt taatgaagcg aatcacttct
    20581 gccagccagg atttacgacc atattccgcc agataaatcc ccgccataat gcccagcggc
    20641 gtaccgaata ccgtggccca caaaattaac agcccgctac ccgccagggc gttcgccaga
    20701 ccaccacctt ccgtattggg cggcggcgtc atttcagtga acagcgccag cgacataccg
    20761 tcgataccgc gagtgatggt ggacattaaa atccagatca gccagaacag gccgaaggcc
    20821 atcgtcgcca tcgagagcgt cagcgcaata cggtttttga ggcggcgacg cgcctgcatt
    20881 ttgcggcgag attcagccag cgccgcagtg gtttgcattt caaccatagc cattagcgtg
    20941 ccccctcatt cttagccagg cgcataatca taaacttcga tgcggcgagg acgatgaagg
    21001 taatcacaaa caggatcagg cccagttcca tcagtgcggc aacgtgcaga ccggattccg
    21061 cttccgcaaa ttcgttcgcc agcgcagagg tgatactgtt gcccggcata tacagcgagg
    21121 cgctgtcgag ctggtaggtg ttaccgatga taaaggtcac cgccatggtt tcaccgagcg
    21181 cgcggcccag ccccagcatg atgccgccga taacaccatt tttggtgaac ggaagaacga
    21241 tacgccagat aacttcccag gtggtgcagc caataccgta ggccgactct ttcatcatca
    21301 ccggggtttg ttcgaacaca tcacgcatta ccgccgcaat gtacggaata atcatgatgg
    21361 cgaggatcac gcctgccgcg aggataccga taccaaatgc ggggccagag aacagcgcgc
    21421 caacaatcgg gatattcgac atgatattgc cgaccggctc ctgaaagtaa acggcgaaca
    21481 gcggcgcaaa gataaacagg ccccacatgc cgtaaacgat acttggaatg gctgccagca
    21541 gctcaatggc gatacccagc gggcgtttca gccagccagg cgcaagctca gtcaggaaca
    21601 gggcgatacc gaaactcacc gggacggcga tcagcagcgc gataaacgaa gtcaccaacg
    21661 taccgtagat cggcaccagc gccccgtaga tatcgttcgg tgcatcccac tctttggtcc
    21721 ataggaaagc cagaccaaat ttctgaatgc tcggccagga ggagatgatc agagagacaa
    21781 taatgccacc caacatcaat agcacaatca gcgccgccag ttttaccagc acgctgaaaa
    21841 ttatgtcgcc ctttttaccc ggtgggttaa aagcaggctt ggttgcagcc ataagttact
    21901 cttcagttaa acgcgtttac aaagtcattt tgtaatgccg gatgcggcgt aaaacaccgt
    21961 acccggcctg gagttttatt agtacagcgg cttaccgcta ctgtctttaa tattggtctt
    22021 ccacgcagcg cgaacctgtt caactacact atccggcagg ctggcgtaat ccaggtcgtt
    22081 cgcctgttta gccccggttt tgtacgccca gtcgaagaat ttcagcactt ctgtgccttg
    22141 ttctggtttc ttctgatctt tgtggatcag aatgaacgtg gtagaggtaa taggccatgc
    22201 atcttcgcct ttctggttgg tcagatcctg agcgaaggtt ttgctccagt ctgcaccttt
    22261 tgctgcatta gcgaagtttt cttcggtcgg actaaccggt ttaccatcag cggagatcag
    22321 tttggtgtac gccaggttgt tctgcttcgc gtaagcatat tcaacataac caattgcacc
    22381 cggcagacgc tgaacgaacg cggcgatacc gtcgttacct ttaccgccca gaccgatcgg
    22441 ccattttacg gtagagccag taccaacgtt gtttttccac tcttcgttca ctttcgccag
    22501 gtagctggtg aagacgaagg aagtcccgga gccatctgcg cggcgtacta cagcaatgtt
    22561 ttgtgaaggc agtttcagac ccggattcag tttggcgatg gcttcatcat cccacttctt
    22621 gattttgccc aggtagatgt cgccgagggt tttaccatcc agcaccagtt cgccagactt
    22681 cagccctgga atgttaaccg ccagcaccac gccgccaatc acggtcggga actggaacag
    22741 accttcctga gccagttttt cgtcagacag cggcgcgtca gaggcaccaa aatcaacggt
    22801 attagcgata atctgtttta cgccaccgga agaaccgata ccctggtagt taactttatt
    22861 accggtttct ttctggtaag tgtcagccca tttggcatac accggcgcag ggaaggttgc
    22921 acctgcacct gtcaggcttg cttctgcaaa cacagagaaa gcgctcatcg ataaggtcgc
    22981 ggcgacaaca gttgcgacgg tggtacgcat aactttcata atgtctcctg ggaggattca
    23041 taaagcattg tttgttggct acgagaagca aaataggaca aacaggtgac agttatatgt
    23101 aaggaatatg acatttttat gacagaaagg aattaccaaa taacttactg attttaaatg
    23161 tgaagatgaa aatcccacat gtcatctttt tgtcatgatg catttaaaag tgataaagaa
    23221 atgggtgaga tgacaacgat gtgatgaaca atcgttgtca gacctattag cgcacaatct
    23281 caacagttat cgtcgctgtt gcagtaaatt gccccgggga tgggcgagct ccagtaaggc
    23341 tcgcaggagc agctgaaaaa gttacactac ctgatttttc accatacact gttgatttac
    23401 ccatgtctgt tggcaactca ccaccgttca ctttgatttc cttattattt ttgtcgtaaa
    23461 tttttatccc cacgttagag tttgaagtta cgaccattga attgtccgta ttgggttcgg
    23521 tagcataaaa agacgtactg atatcatcgg tattcagtat ccccaggttt tcacactcaa
    23581 tagtgaccgt tttagtaatt ttatgtgttg ttaccgcact tccaacaaca gatgaaaaat
    23641 ctgcagctgg tatttcttta aaatcaaaat aaactggctg taaattattt atttcgcaac
    23701 tttgcggagc tgtaatatcg ccccgaatat aaacatcagc catcggctgt gagtaatcca
    23761 ccggggtgtc acggctaatt gttccataca atgtcactat ctttgtttca ggaataatca
    23821 attttcctgc aaatgtcttc ttgatataaa aatatactat cgctttagcc ccactggata
    23881 tacttaaagg ttcaatgcgg ttgcaattgt actttgtatt accagacggg ttgttagcat
    23941 gatattcaaa aggtgcattc acatacccaa ccccaagaat atctatagca atcccaacat
    24001 caagattcgg attaagatca taataattaa tattagagtg aaccccagca taagttaatg
    24061 cctggtttat ttttgacgta taatagatat aggggaattc tcctacgtta ccagtatcac
    24121 aatgacagcg tccaggatat gaggatccat tagttacagt ggtgttaaat gtcgttccaa
    24181 ccttattttc gttcgcggta ataagatatc ccgagaaatc aagcgtactg tgaaatacgc
    24241 cattatcagg ggtacagata cctttaggag ctgcaagtgc tacactactt aacgagagca
    24301 taaacacagc aaagcaccac tgtttgatat acttgttcat aaaaatcctt ttaatgacat
    24361 gtcacaccct gttcaattaa tccgctctca ctttctggtg tcgaaagtga ataattgaag
    24421 atgcattttg ttgaggcgtt ttctccccag gataacgtaa cctgtccttt ttctggtaga
    24481 ccagaaagat aaagttcgcc acggtcaccg acaattcctg aatctgccaa acttgcatct
    24541 tgtatcgcag caatggctcc aaaaggtacc ggtttaccat ttgcatttaa cactctgaag
    24601 agaacgcgat aacccacatg agtcacaaac tccgctttaa ccactgcacc acgcgtggaa
    24661 accatcgtaa cgacggtatt atcaagttct atattcgact tcgagaaata gttatcgctg
    24721 attgctaccc gattttcatc ataagggttt agctgtgtct tgaccgtata gccgcgccag
    24781 tccgtcgcag caccgcgcat attatctatt tctaacccgg cggccccagg tgctttaaca
    24841 agagcaatag tttcacctaa ctcctgactt aacgttaccc cttcgctgtg tgccagaatt
    24901 ccccctctga taccataatt cagcctctgg gaattttggc tgtagttata gctgccgttc
    24961 accgtgccat aagagccatc ataggtcagg gacatattac cgccaacctc atcatcatga
    25021 ctgttatagg actgcattaa attccatgaa aggttatttc gttccagcgc cgtaccgctg
    25081 agtccgagtt gatgtgagct accggaatta tcgttttgcg aaacagaata ggtggcgtaa
    25141 ctattacccc accagcccga taaaggaaca gataaagaga gcatgtagat ctgctcactg
    25201 gtgttatcac tggcatggtt gttccccgtc ccctgtctgg cagtattgga ataactccag
    25261 gagacgttcc acgagagacg cttccagcta ctgctgtagc caaattgcat taatcgttcg
    25321 acatcatcgg tatgccagta ttgttgccgg cttaatgtca ggtataaatt gccataatct
    25381 ctccccaacg actgcgttag cgttccctcc acgcgactac gtcggttacc tgtttcccaa
    25441 aagtcttcag ggctgttgcg ccgcgatgcc cactcattga gggtatagta gccctcggtg
    25501 gagtaacgat agccaaccag gcgcaagcta gtgtctgtgt cgtcaaatcg ttttgaatag
    25561 agaaagcgat atgactgacc cgtttctgta tcatcatggc taaaattcgc acgagcatgt
    25621 gtcacatcga aagaaattgc gcccaaagcg ccaagatctt taccgacacc cagagctgca
    25681 gaaccataat tttcagcagc ctgcatacct ccatataaag tgatcccgtg ggaaaaacca
    25741 tgaagtattt gcgcctgaag gacgtccggt gtaaatccgt cttcatcgcg caattcaccc
    25801 acgctgacat caacatctgt ctgaccttca cgtttaagaa tcgccaatga agcataaggt
    25861 tgggtgaatc gttgttcgct accgtcttct tctttgatgg ttacgtccaa atcgccacct
    25921 gtactggcgg tgttgagatc ggtgatctca aaagcacctt gtggaacggc agactgataa
    25981 atcacgtagc cattctgccg aatgatgacg gtggcgttgg actttgcaat cccacgaacc
    26041 acaggagcaa agccattctg gctggcgggt agcatatcgt tatcagtata caaacgcgcg
    26101 ccgcgaattt gcgtactgtc gaaaatatcg ctcgccgtcc acgtatcacc aatcataatc
    26161 tggcttctta acgcggcaat atcgcgctgt atccaggtac taatggagtt gtattccgca
    26221 cggccatcgc tggtgtttaa agtactataa ttacgtagcc gccacggtcc aatattcagt
    26281 ccattgcgca gattgagata actggtatct gtctcttgtg aaccataatc tgaacgtagt
    26341 gtatgtgaac cagaaaaatc gtagttgatg gtgagcgcgt taatcccctc atcccaacgc
    26401 gtggggctga tgtaatcata agccatcgcg tccatgtaga tttggggaat ggatatattc
    26461 agctgcaatt tatcactgtc aaactgccag gatgagcctt caatacgtgg agcaggatcg
    26521 aggcaaaccg tctcatccac atcattgaac gatttgagcg catcaacttt tatgccaaag
    26581 gaggacagtg tttttttaat ggcaataaag caagcgtgca gatcagcatc tggattatcc
    26641 tgaacaaatg tcacatcctg acgttcaaac attttaccgt tgataaaaat atcaacgcta
    26701 taaacacctg ggggcatcag gtttgcccga ttaaactggc gaatatcgac tccggattga
    26761 tggtcgattt ccagaagcgc cggattgaat acactgacct cggccatcag gttttgcgag
    26821 aaaatcagac acgtccctgc cgtgaggcca accactattc tggtcgtcat cataatacgc
    26881 ctatccttat cccttgctta cagcgtcatt tttatcgggt cactgactgc gccgtaatca
    26941 ttaataaatc gccaggtcat ttggcctccc gcagaattag caggtaaagg taacgtgagc
    27001 gtttcaaaag gacggatcat attgaggggc acatcatggc catcaacgat gacatccatc
    27061 acactgatat gaattgggtt gggattctct gctatcagat ttcgcccttc ccggcgccat
    27121 ttcactttcg gcgcagcatc ttcgggaata aggcggatat ttgctggtcg ccagaacagc
    27181 ttgatacgcg tttttactgc tatttccagc gagttcgtcg ctttgttgct cgctgatggc
    27241 ggaatatttt taatattcag ccagaagact gactcgcgat catcaggcaa tgacacacct
    27301 ttggtgtgaa taatgcgtaa tattttttct tgcgaaggtt ctaatcgtga tactggcgga
    27361 ataacggtaa acggcgcttt gcttttatcc tctttattaa atggatctac ccaagcctgg
    27421 atgagataag gtattttttc ttctttgcta aatacagaaa tagatttatc agcattatta
    27481 cttaagtaaa ccacccgcgt accaccaatg acaacgcctg cataagttaa tgctggttgg
    27541 cctaaaaaaa cgctgagtat aatcgcggta aataacttac atgaaactgt tgttttaaag
    27601 agcctgatta aacaactttt tctggacata acgactccct ggccaaagcc aactgaataa
    27661 aaaggccctt gataaattat ttcttatatt cgacagtaaa ttgtgaatca gcgttcgccg
    27721 taccagccgt tacggtcgca ttggttgcca catagcgtgc aataaacttc atttcagcct
    27781 tgccttcagc atcgatagcg gtataagcag agtcgttata aagtttaacc tgcgaattat
    27841 cggactggtt aaaaatacga ataccaacgc cggttgccgc aatcgcttgt cctgtaccgg
    27901 taaaatcacc agggttgcct gctgtaagcg gcgtacccac tttcaggtcg ccgtcgccgc
    27961 caggcgcttc tgtaccatca aaacgaacgg ctgcttgagt gtacgttgcc gggcaatttt
    28021 caagcttaat actgaacttc tgtggcgacg ccagactacc tacacctgaa aatgcatttt
    28081 tactgacttt acccagtggg acaatttgat cggctgtatc ggaggtaact tcacaagtag
    28141 tgtcgacaat ttcaccgacg aagtgaacaa caccatcttc tgccagtgca tgcccggcca
    28201 gcattagaga tgccagagtg aatgcaccgc ctataatatt acgcttcata aaaactccat
    28261 tgatataata tatcctttac cat
//
LOCUS       DAZGOB010000058        27411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGOB010000058 DAZGOB010000000
VERSION     DAZGOB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.058"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..614
                     /locus_tag="ACHLIF_002617"
     CDS             <1..614
                     /locus_tag="ACHLIF_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO9606612.1"
                     /translation="INFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(653..1552)
                     /gene="nhaR"
                     /locus_tag="ACHLIF_002618"
     CDS             complement(653..1552)
                     /gene="nhaR"
                     /locus_tag="ACHLIF_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO9606613.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(1618..2784)
                     /gene="nhaA"
                     /locus_tag="ACHLIF_002619"
     CDS             complement(1618..2784)
                     /gene="nhaA"
                     /locus_tag="ACHLIF_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO9606614.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3370..3522
                     /gene="mokC"
                     /locus_tag="ACHLIF_002620"
     CDS             3370..3522
                     /gene="mokC"
                     /locus_tag="ACHLIF_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO9606615.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3626..4756)
                     /gene="dnaJ"
                     /locus_tag="ACHLIF_002621"
     CDS             complement(3626..4756)
                     /gene="dnaJ"
                     /locus_tag="ACHLIF_002621"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO9606616.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4845..6761)
                     /gene="dnaK"
                     /locus_tag="ACHLIF_002622"
     CDS             complement(4845..6761)
                     /gene="dnaK"
                     /locus_tag="ACHLIF_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO9606617.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            7138..7542
                     /gene="yaaI"
                     /locus_tag="ACHLIF_002623"
     CDS             7138..7542
                     /gene="yaaI"
                     /locus_tag="ACHLIF_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO9606618.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7568..8281
                     /gene="msyB"
                     /locus_tag="ACHLIF_002624"
     CDS             7568..8281
                     /gene="msyB"
                     /locus_tag="ACHLIF_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO9606619.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELAELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8430..8996
                     /gene="satP"
                     /locus_tag="ACHLIF_002625"
     CDS             8430..8996
                     /gene="satP"
                     /locus_tag="ACHLIF_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO9606620.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(9031..9618)
                     /gene="mog"
                     /locus_tag="ACHLIF_002626"
     CDS             complement(9031..9618)
                     /gene="mog"
                     /locus_tag="ACHLIF_002626"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO9606621.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9733..10686)
                     /gene="tal"
                     /locus_tag="ACHLIF_002627"
     CDS             complement(9733..10686)
                     /gene="tal"
                     /locus_tag="ACHLIF_002627"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO9606622.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10965..12395
                     /gene="yaaJ"
                     /locus_tag="ACHLIF_002628"
     CDS             10965..12395
                     /gene="yaaJ"
                     /locus_tag="ACHLIF_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO9606623.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     IQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            12465..13241
                     /gene="yaaA"
                     /locus_tag="ACHLIF_002629"
     CDS             12465..13241
                     /gene="yaaA"
                     /locus_tag="ACHLIF_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO9606624.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(13394..13690)
                     /gene="yaaX"
                     /locus_tag="ACHLIF_002630"
     CDS             complement(13394..13690)
                     /gene="yaaX"
                     /locus_tag="ACHLIF_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO9606625.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(13904..15190)
                     /gene="thrC"
                     /locus_tag="ACHLIF_002631"
     CDS             complement(13904..15190)
                     /gene="thrC"
                     /locus_tag="ACHLIF_002631"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO9606626.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(15191..16123)
                     /gene="thrB"
                     /locus_tag="ACHLIF_002632"
     CDS             complement(15191..16123)
                     /gene="thrB"
                     /locus_tag="ACHLIF_002632"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO9606627.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAEIF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(16125..18587)
                     /gene="thrA"
                     /locus_tag="ACHLIF_002633"
     CDS             complement(16125..18587)
                     /gene="thrA"
                     /locus_tag="ACHLIF_002633"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO9606628.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDRFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18614..18733)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18669..18734)
                     /gene="thrL"
                     /locus_tag="ACHLIF_002634"
     CDS             complement(18669..18734)
                     /gene="thrL"
                     /locus_tag="ACHLIF_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO9606629.1"
                     /translation="MKRISTTIITTITITTGNGAG"
     gene            complement(18948..19634)
                     /gene="yjtD"
                     /locus_tag="ACHLIF_002635"
     CDS             complement(18948..19634)
                     /gene="yjtD"
                     /locus_tag="ACHLIF_002635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO9606630.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(20034..20174)
                     /gene="yjjY"
                     /locus_tag="ACHLIF_002636"
     CDS             complement(20034..20174)
                     /gene="yjjY"
                     /locus_tag="ACHLIF_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO9606631.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20270..20986
                     /gene="arcA"
                     /locus_tag="ACHLIF_002637"
     CDS             20270..20986
                     /gene="arcA"
                     /locus_tag="ACHLIF_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO9606632.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(21046..22398)
                     /gene="creD"
                     /locus_tag="ACHLIF_002638"
     CDS             complement(21046..22398)
                     /gene="creD"
                     /locus_tag="ACHLIF_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO9606633.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(22456..23880)
                     /gene="creC"
                     /locus_tag="ACHLIF_002639"
     CDS             complement(22456..23880)
                     /gene="creC"
                     /locus_tag="ACHLIF_002639"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO9606634.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23880..24569)
                     /gene="creB"
                     /locus_tag="ACHLIF_002640"
     CDS             complement(23880..24569)
                     /gene="creB"
                     /locus_tag="ACHLIF_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO9606635.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTIDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24582..25055)
                     /gene="creA"
                     /locus_tag="ACHLIF_002641"
     CDS             complement(24582..25055)
                     /gene="creA"
                     /locus_tag="ACHLIF_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO9606636.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25266..26135
                     /gene="robA"
                     /locus_tag="ACHLIF_002642"
     CDS             25266..26135
                     /gene="robA"
                     /locus_tag="ACHLIF_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO9606637.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(26132..26779)
                     /gene="gpmB"
                     /locus_tag="ACHLIF_002643"
     CDS             complement(26132..26779)
                     /gene="gpmB"
                     /locus_tag="ACHLIF_002643"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO9606638.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26831..27352
                     /gene="yjjX"
                     /locus_tag="ACHLIF_002644"
     CDS             26831..27352
                     /gene="yjjX"
                     /locus_tag="ACHLIF_002644"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO9606639.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
BASE COUNT         6698 a         7249 c         6643 g         6821 t
ORIGIN      
        1 cgataaattt caccattcca tattctatcg catctgtatc agatagttct aacaccgcag
       61 acatttttcc tggaactcct tttcctttgt tgttcgaaat tgaagtgagt tcatgtacag
      121 ggaataatac taattgtaat agcgtccgct ttaattataa tttaaatact attttacagg
      181 taaatttaat gacctgtggt tttgaagatc aatccatcga cactggacga tttgtactgt
      241 ctcgtatcaa taatgaaaat tatcagtttc atcatataag atttgactgc atccagggcg
      301 agcaaggtga attagttttt gaacccagtg atgttgaata ttactttgaa ccagtgacga
      361 tacaggagtc aggaaccagc atattaaaaa atgagttaga aaacagtgag gacggtgcgg
      421 gtcaaatagg ctttcaactc tccaatgatg gtacgaatga aatccagtat ggtaaaagca
      481 attactactc ttttcagcat ccccatgaag gaagtaatca gattcctcta tttattcgcc
      541 cgcgtacata tggaaataat gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg
      601 ttatgtacaa ctaatctatc tcccccacct taactttggg gggaggtgcc gattaacgcg
      661 ccgctggact aaaaagcgca gaataatccg tattgcagat tcgctgtacc gccgggtgct
      721 gaatcatccg ctcagcaaaa atagcatggt actcttccat cacattctcg acgcgaccaa
      781 tttctacgac agttttatcg gcataaaagt catatgcata aagcgttggg gcaacgaaga
      841 ttgcattgtg catcgcacca aaagctttca tcaacgcggc atcatcaaac tcgccgagga
      901 tttctacgtt taatccctgg gagttaaacc agttaagcaa tttgcgccct aacattgaac
      961 gtcgcccagg aatcaaaagt cgccgttctt ccagacaagc cgggaacggt ttttctggtg
     1021 gtggatttgt acaccagaaa ctcacgccac attcgccaat tctcacggag aacaggcctt
     1081 cctgctgcgt agagtctatc ggacagtcag aaatgatcat atccagttta tgctgactta
     1141 attgctccag cagcatttcg tgggtggatt cgaagcagcg aagatgaatg ggctcgcctt
     1201 ctaccactgc ggcgttaagt acgctactga ccaggcgttt ggaaagtgca tcagccacac
     1261 caacgtcaaa caataaattg gattctttgc gatagttcac aatatccagc atttcctggc
     1321 ttaaggtgaa cattttatcg gcatagcgat agaccagttc tcccagctcg ctgggttcga
     1381 gaccacgtcc cttgcgttta aataatttgc cttgcaggcg ctcttccagc gctcgaatct
     1441 gtccggtaat ggtttgtgga gttaaataaa gcgcctccgc tgcgccaacc acggaacctt
     1501 ctttatagac atgccagaag taatacaagt ggttgtaatt gatatgagac atgctcattt
     1561 ctctccctga taacaatgaa aagggagccg tttatggctc cccggtaaac cgtcctgtca
     1621 aactgatgga cgcaaacgaa cgcgtaacca gctgtatcca attaccgccg aagagataga
     1681 accgaccagg ataccgagtt tcgcccagtt aatcagttct ggatctacgc taccaaaggc
     1741 caggctggca ataaagatag acatagtaaa accgataccg cacaggatcc ccaccgccat
     1801 aatttgctga taagtcgttc cctcaggcag atgcgccagt ttcaaacgca gcgccaacca
     1861 gcagaacaga ctaatcccca gcggtttgcc aatcagcaag ccagcgatga tccccaatgg
     1921 cagaatggag gtcaagccat ccagcgtgac accttgcagt gaaacgccag cattagcaaa
     1981 tgcaaacagc ggcaaaatca gatacgccac ccacgggtgt aacacatgct ccagtcgctt
     2041 cgctggagaa cgcccatgct tctctttcaa aggaataaag aagccgacaa ttacccccgc
     2101 cagggttgcg tgaacccccg atttcaacac cgcagtccac aacactacgc caaccagaat
     2161 atagacgccc gtgcggcgta caccacacag attcaatacc gcgagtaccg caattgctac
     2221 agccgcgacg ccaagagagg ccatcgataa gtcattagtg tagaacaatg cgatgataat
     2281 gatggcccca agatcgtcga taatagccag agccatcaaa aagatcttca gcgctaacgg
     2341 aacacgactt cccaacagcg ccagcacacc aagtgcaaag gcaatgtcag tagccgccgg
     2401 gatcgcccac ccttcgcggg taatcggatc ggcatagtta aaggccagat agagtaatgc
     2461 cggaacaatc atcccaccaa tagcggcgat aactggaaat gcggcctggc gtaagctggc
     2521 tagcgatcct tgcatcagtt cacgtttaac ttccagaccg actaacagga aaaataccgc
     2581 catcagcgcg tcatttatcc ataacagcat gtttttgttg atttcgagtg aaccaacccg
     2641 gagctgaacc ggcgtctcca gaaagtcgtg ataccatcca ctggttgcgc cgctattggc
     2701 cataatcatc gccaggatag cggcaatgat aagaataatg cctcccgagg catcactgct
     2761 aaagaatcga tgcagatgtt tcacttttta tttctctttc aggtgaatag atcgttttta
     2821 ctattttacc ccgcacaaat catcgttaaa atcagattat agtgtaggaa taactcgttt
     2881 tttacgatcc attgaagaat ttgcgccatc agcggtgaga tgagtttttg acggctgtaa
     2941 gcgctcacga gtgaccctaa gcccagataa tcatagctta aggtgaggat ttctgaggga
     3001 ttaattagga aaagtaataa ttaatttctt gtcagtcggc gttatttgtt aggacaatat
     3061 ttcaaaaata atccagcttg tcgtcaataa ttgcgataaa gagataaatt aaagttaaat
     3121 aattaaatca ttaaaaaatt acgccacaaa aatgcgaggc gtcttcagga ttagaatcga
     3181 taaaacagaa tgggttaaag agagcgatat aatagcggcg ggtacttgag gctatctgtc
     3241 tcaggcatta gctgaacggc agatagagaa aagccccgag tgatatttta ctatcaaccc
     3301 gaggccccct atatgctgaa cacatgtaga gtgcctctta ctgaccgtaa ggtcaaggag
     3361 aagagagcaa tgaagcagca taaggcgatg attgtcgccc tgatcgtcat ctgtatcacc
     3421 gccgtagtgg cggcgctggt aacgagaaaa gacctctgtg aggttcacat ccgaactggc
     3481 cagacggagg ttgctgtttt cacggcttac gaatccgagt aagtgcaacg gcggggagtg
     3541 atccccgcca cttttcaggt gctcgcatat cttcaacgca ccctaatttt acccaagcct
     3601 gcccacgggc aggcttttgg ggaggttagc gggtcaggtc gtcaaaaaac ttcttcacac
     3661 catcaaagaa gctctttgag cgcgggctgt tgtgctcacc ggttgggcca ccgaagcttt
     3721 cttgcagctc ttgcagcagc tgtttctgct tctcgttcag gcctaccggt gtttcgacga
     3781 caacgcggca cagcaaatca ccctgtgcgc caccgcggac agacttgacg cctttaccgc
     3841 gcatacggaa aagcttaccg gtctgggttt cgccaggcac tttcagtttg acgcgaccat
     3901 caagggtcgg tacttcgatt tcgccaccca gcgccgccat agcgaagttg atcgggactt
     3961 cgcaatagag gttgttgcct tcacgctcga aaatcgggtg ctgtttaacc tgaacctgaa
     4021 cgtacagatc gcctgccggt gcgccatgct cgcccgcttc accttcgccc gcaagacgga
     4081 tgcggtctcc agtgtccacc cctgccggga ttttaacgga cagcgttttg ctgcgctcaa
     4141 cacgaccatg accatgacat ttgttgcacg gatctttgat cagcgtaccg cggccctgac
     4201 agtgtggaca ggtctgctgt acagcgaaga atccctgacg catctgcacc tgaccagaac
     4261 catgacaggt cggacaggtc tgcggctgtg tacctggttt tgcaccgcta ccgtggcaaa
     4321 cgtcacactc ttccagagtc ggaatgcgga tctctttggt cacgccacgt acagcttctt
     4381 cgagggtgag ctccatgtta tagcgtaaat cagcaccgcg cgccgcacgt tgacgaccac
     4441 gtccgccgcc aaaaatatcg ccgaaaacgt caccaaaaat atcgctgaag tctgcgccgc
     4501 cgccaaaacc gccgccgccc ataccacctt gctcaaacgc agcatgacca tactgatcgt
     4561 atgccgcacg tttttgcgag tcggtcagaa cttcataagc ttccttgatc tctttaaatt
     4621 tcgcctcggc ctctttgtca ccctggttac ggtccgggtg gtatttcatg gccaggcgtt
     4681 tgtaggcctt tttgatttca cgctcttccg ctgttttgga aacgcctaaa atctcgtaat
     4741 aatcttgctt agccatcttt tttaaattgc ccctagatga atgcacgggc ggagaggaaa
     4801 ttccccttcg cccgtgtcag tataattacc cgtttatagg gcgattattt tttgtctttg
     4861 acttcttcaa attcagcgtc gacaacatcg tcatctttcg cgttgtttgc agaagcatca
     4921 gcaccggcag tctgctgctg ggcatgttgc tgctgggcga tttccatcag tttctgggaa
     4981 acctgtgcca gctcctgcat tttcgcttcg atagcggctt tgtcttcacc tttcagagca
     5041 gtttccagtg cagtcagtgc agactcgata gcagttttgt cgtcagccgg cagtttgtcg
     5101 cctgcttctt caacctgctt acgggtgctg tgcagcagat ggtcgccctg gttgcgagtc
     5161 tgtaccagct cttcaaactt acggtcagct tcggcgttag cttctgcgtc gcgtaccatt
     5221 ttctggattt catcttcgtt cagaccagaa gaagccttga tggtgatctt ctgctcttta
     5281 ccgctgtttt tatctttcgc ggaaacgtgc aggataccgt cagcatcgat atcgaaggta
     5341 acttcgatct gcggcatgcc gcgcggtgcc gggttgatac catccaggtt gaactgaccc
     5401 agagatttgt tatcagccgc acgtttacgt tcaccctgca gcacatggat ggttaccgca
     5461 gactggttgt cttcagcggt agagaacacc tggctgtgct tggtcgggat agtggtgttt
     5521 ttcgcgatca gcgtcgtcat cacaccgccc atggtttcga tacccagaga cagcggggta
     5581 acgtccagca gcagtacgtc tttcacgtca ccagtcagaa caccaccctg aacagcagca
     5641 ccgattgcta cagcttcgtc cgggttaacg tctttacgcg gctctttacc aaagaactca
     5701 gcaactttct tctgaaccat tggcatacga gtctgaccac caacgaggat aacgtcgtcg
     5761 atatcagata cggacaggcc agcgtcctgc agtgcaactt tcagcggctc aatggaacgg
     5821 tttaccagat cttcaaccag gctttccagt ttcgcacgag tcactttgat gttcatgtgt
     5881 ttcggaccgg tcgcatctgc agtgatgtac ggcaggttaa cgtcggtctg ctgagcggaa
     5941 gacagttcga ttttcgcttt ttctgccgct tctttcaggc gctgcattgc cagcggatcg
     6001 ttgcgcaggt caatgccctg atctttcttg aattcttcaa ccaggtagtt gatcagacgg
     6061 ctgtcgaagt cttcaccacc caggtgggta tcaccgttgg tagccagaac ttcgaaggtt
     6121 ttttcgccgt caacttcgtc gatttcgata atagaaatat cgaaagtacc accccccagg
     6181 tcataaaccg cgatagtacg gttgccagta cctttgtcca gaccgtaagc cagcgcagct
     6241 gcggtcggtt cgttgatgat acgttttact tccagaccag cgatacggcc tgcgtctttg
     6301 gttgcctgac gctgagcatc gttaaagtat gccggtacgg tgataacagc ttcagttacc
     6361 ggttcaccca agtaatcttc agcggttttc ttcatttttt tcagcacttc agcagaaatc
     6421 tgcggcggtg ccattttctg gcctttaact tcgacccatg cgtcgccgtt atcagcagca
     6481 ataattttga acggcatgat ggaaacatca cgctgtactt cttcgtcctg gaagcggcgg
     6541 ccaatcaggc gtttaatcgc aaacagggtg ttttgcgggt tcgtcactgc ctgacgttta
     6601 gccggctgac caaccagagt ttcaccatcc tgggtatagg caataataga aggcgtggtg
     6661 cgatcgcctt cggcgttctc cagcacgcga ggagtggtgc catccataat cgctacacaa
     6721 gagttggtag tacccaggtc gataccaatt attttaccca tctaaacgtc tccactatat
     6781 attcggtcat catgtggttg tgagtctgta ataggggcga aacgtctggt ttcaactgcc
     6841 caatttcatt tttttgcaga ctcactcact gcggttgact actaaatggg gtcgtaaccc
     6901 acgtcatcaa gggggagatg caaaaatttt ttgtgctttt gcgcagaaat ttcgcgatat
     6961 agcaggacaa gacgtgaaac tgacagcaat tttattcata taaagaatga atcgcagcca
     7021 agacattcat caaaaaattg taataaaaag aaaacattac gcacctgaat cttctcttta
     7081 tcagcagtaa actagtgggt atccatcccc ctacctctcc ccactaagag aatccttatg
     7141 aaatccgttt ttacgatttc cgccagcctg gcgattagtc tgatgctgtg ctgcacggcg
     7201 caggcaaacg accataaaat cctcggcgtc attgcgatgc cacgtaacga aaccaacgat
     7261 ctggcgctga aacttcctgt ttgccgaatt gttaaacgca tacaactctc tgccgaccat
     7321 ggcgatttac agttaagcgg tgcatcggtt tatttcaaag ccgcccgtag cgccagtcag
     7381 agcctgaata ttccttcaga aataaaagaa gggcaaacca ctgactggat caacattaac
     7441 agcgataacg acaataaacg ctgcgtctca aaaatcacct tttccggtca tacggtgaac
     7501 tcatcggata tggccacgct gaaaattatc ggcgacgact aacgccagaa tatagaagcc
     7561 acaaaaaatg aatgttaatt acctgaatga ttcagatctg gattttctcc agcattgcag
     7621 tgaggaacag ttggcaaatt tcgcccgatt gctcacccat aatgaaaaag gcaaaactcg
     7681 cctctccagc gtactgatgc gcaacgaact gtttaaatct atggaagggc atcccgagca
     7741 acatcgccgc aactggcagc tgattgctgg agaattacag cattttggtg gcgatagtat
     7801 cgccaacaaa ctgcgcggac acggtaaatt gtatcgggcc attttgctcg atgtttcaaa
     7861 acgattgaag ctgaaagccg acaaagagat gtctacgttt gagattgagc aacagttact
     7921 ggaacaattt ctgcgtaaca cctggaagaa tatggacgag gaacataaac aggagtttct
     7981 gcacgcggtc gatgcgaggg tgaatgagct ggcagagctg ctgccgctgc tgatgaaaga
     8041 caaattattg gcaaaaggcg tgtcgcattt gctttccagc caactgaccc gcattttacg
     8101 cacccacgca gcaatgagcg tacttgggca tggtttgctg cgcggcgcgg ggctgggagg
     8161 ccctgtaggt gcggcactaa atggggttaa agcggtcagc ggcagcgcct atcgcgtgac
     8221 gattccagcc gtactgcaaa tcgcctgcct gcgccggatg gttagcgcca ctcaggtctg
     8281 ataccggaaa ccggggaaaa ttttcatttt cccctcaaaa gatcgtagac actgccccac
     8341 tggctgatta ttatgccgcg ccctgaaaac actacagtta ttcagggaaa ttatttcacc
     8401 attcattcga tgatgatttt tgaggaatta tgggcaacac taagttggct aatccggcac
     8461 cgctgggcct gatgggcttc ggcatgacca ccattctgct taacctgcac aacgtgggtt
     8521 atttcgctct ggacggtatt attcttgcca tgggcatttt ctacggcggc atcgcgcaaa
     8581 tttttgccgg tctgctggag tacaaaaaag gcaacacttt cggtttaacc gcattcacct
     8641 cttacggttc tttctggctg acgttggttg cgattctgct gatgccgaaa ctgggtctga
     8701 ccgatgcgcc aaatgcacag ttccttggtg tctacctggg tctgtggggc gtatttacgc
     8761 tgtttatgtt cttcggcacg ctgaaaggcg cacgcgttct gcaattcgtt ttctttagcc
     8821 tgactgtgct gtttgccctg ctggcgatcg gtaacattgc cggtaacgcc gcaatcatcc
     8881 actttgccgg ctggattggt ctgatctgtg gtgccagtgc aatctatctg gcgatgggtg
     8941 aagtactgaa cgagcagttt ggtcgcaccg ttctgccgat tggtgaatcc cactaattgt
     9001 tttgagatcc ccccgctcgg ggggattttt ttattcgcta acgtcgcgtc ttgcactctt
     9061 cggtctgaat gcagcaacca cttctggtgc cgtttcaacg tatggccctt ccagcaactg
     9121 aatgcagtac ggtacgctgg caaaaatacc gtgtaccaca acgttacccg cagcgtcctt
     9181 cacaccttcc agcgtctctt taatagactt cggctgaccg ggtaagttaa ggatcagcgc
     9241 ctgtttgcga atcacaccaa cctgacgcga aaggatcgca gttggtacaa aatgcaggct
     9301 tatctggcgc atctgttcac caaagccagg catctcgcgg tccgctactg ccagcgtcgc
     9361 atcgggcgtt acgtcacgac gcgccgggcc agttccgccc gtggtgagca ccagatggca
     9421 actcatttca tccactagct cacacaacgt ttgctcgatg atcgcctgct catcggggat
     9481 taagcgggtt tccagttcaa acggcgtggt tagcgccgat gtcagccatt cttccagcgc
     9541 agggatgcct ttatcctgat aaacgccgct ggatgcgcga tcagagatgg aaactaagcc
     9601 aatacgtaaa gtattcatca agatttttcc gtttaaacag tctcgttaaa cagaatgata
     9661 cactgcgaag ggagtgacag acaggcttca gaagaggtag cgtgaccgac ttcccggtca
     9721 cgctaagaat gattacagca gatcgccgat cattttttcc agtttttcct ggtcaatagc
     9781 aaacttacgg ataccttccg ccagtttatc tactgccatt ggatcctggt tgtgctgcca
     9841 caggaactcg gactcagtga tacgcgccgg acgcgctttc acttcgccgg tgtaagacag
     9901 tttacgttcg atagcccctt cgctctcagc cagctctttc agcagtgtag gtgcgatggt
     9961 aagacggtcg cagcctgcca gttccagaat ttcgccgatg ttacggaagc ttgcgcccat
    10021 aaccacggtt tcataaccgt gctctttgta gtactggtag atttcagata cagaaaccac
    10081 gcccggatct tctgccggag cgtactcttt cttatcggta ttcgctttgt accagtcaag
    10141 aatacggcca acaaacggcg agatcaggaa cacgcccgct tccgcacaag cacgagcctg
    10201 agcgaaggag aacagcaggg tcaggttaca gttgatgcct tctttttcca gctgttctgc
    10261 agcacggata ccctgccagg tagaagccag tttgatcaga atacgatcgt tgctaatacc
    10321 agcatcgttg tagagtttga tcaggcgttt tgctttcgca attgacgctt cggtgtcata
    10381 ggaaagacgc gcatcaactt cagttgagat acggcccgga accagtttca ggatttccag
    10441 accaatattt actgccagtt tgtcggtcgc gtccacgatc tgctgcgcgc gatcgttgct
    10501 ctgctgtttc gcccaggcga cagcatcatc aatcaactta cggtattccg gaatctgcgc
    10561 tgcgttaaga atgagagaag ggttggttgt ggcatcctgc ggttgataca gcttcattgc
    10621 cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga cgaagggagg tcaatttgtc
    10681 cgtcatgata gtatttctct ttaaacagct tgttaggggg atgtaaccgg tctgccctga
    10741 tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac ggttatcgcc aggcgagact
    10801 gtttcggatt tctgacactg cgttgatatc gcccgccatt tttatacaaa acctcatgta
    10861 tgctacgcag aagttatcaa gtacctcgta gcgtatatac ttcttaaaca attggtaacg
    10921 tttacacagg aaagtcatcg cgaccggcaa taagagggat atgcatgcca gattttttct
    10981 cctttattaa cagcgtcctt tggggatcgg taatgattta cctgctcttc ggcgcaggtt
    11041 gttggttcac ttttcgcacc ggatttgtgc agtttcgcta catccgccag tttggcaaaa
    11101 gtcttaaaaa tagcattcat ccacagccag gcggtttaac ctcatttcag tcattgtgta
    11161 ccagtcttgc ggcgcgcgtg ggtagcggca acctggccgg cgttgcgctg gcaattaccg
    11221 ccggtggacc tggagccgtc ttctggatgt gggttgccgc gtttatcggc atggcgacct
    11281 cgtttgccga atgttccctc gcacagcttt ataaagaacg tgatgccaat gggcagtttc
    11341 gtggcggacc ggcatggtat atggcgcgcg ggctggggat gcgctggatg ggcgttctgt
    11401 tcgccgtctt tttgctcatc gcctacggca taattttcag cggaattcag gcgaacgccg
    11461 ttgcgcgagc cctgagtttt tcttttgatt ttcccccgct ggtgacaggt attattctcg
    11521 ctgtctttgc tctgctggcg atcactcgcg gtcttcatgg cgtcgcccgg ctcatgcagg
    11581 ggtttgtccc gttgatggcg ataatctggg tactgaccag cctggtgatt tgcgtaatga
    11641 atatcgggca acttccccac gtcatttggt ctatttttga gagtgctttt ggctggcagg
    11701 aagcggcagg cggcgcggcg ggatatacct taagccaggc gattactaac ggttttcagc
    11761 gcagtatgtt ttccaatgag gcgggaatgg ggtcgacgcc aaacgcggca gcggcagcgg
    11821 cgtcctggcc tccgcatccg gcagcgcaag ggattgtcca gatgattggc atttttatcg
    11881 acaccctggt catctgtacg gcaagcgcca tgctgatatt actggcgggt aacggcacaa
    11941 cctacatgcc gctggaaggt attcagctta tccagaaggc gatgcgggtg ctaatgggtt
    12001 cctggggtgc tgagtttgtt accctcgtgg ttattctgtt tgccttcagc tccatcgttg
    12061 ccaactacat ttacgccgaa aacaatctct tctttttacg cctgaacaac cctaaagcga
    12121 tctggtgttt gcggatctgc accttcgcaa cggtcatcgg cggcaccttg ctaagtcttc
    12181 cgctgatgtg gcaactggca gatatcataa tggcctgcat ggctattacc aatttgaccg
    12241 ccattttact gctctcgcct gtggttcata ccattgccag tgattatcta cgccagcgta
    12301 aactcggcgt gcgcccggtg tttgatccgt tgcgttatcc ggatatcggc cgccagcttt
    12361 ctccggacgc gtgggatgat gtttcgcagg agtaatcaca actatcgatc aactcattct
    12421 ctttttttgc taaagtcggc ataaatttcc tgcaaggact ggatatgctg attcttattt
    12481 cacctgcgaa aacgcttgat taccaaagcc cgttgaccac cacgcgctat acgctgccgg
    12541 agctgttaga caattcccag cagttgatcc atgaagcgcg gaaactgacg cctccgcaga
    12601 ttagcacgct gatgcgcatc agcgacaaac tggcgggtat caacgccgct cgctttcatg
    12661 actggcagcc agatttcacg ccggagaatg cccgccaggc gattctggcg tttaaaggtg
    12721 atgtctacac cggcttgcag gccgaaacct tcagcgaaga cgatttcgat tttgcccaac
    12781 agcatttgcg aatgctttcc ggcttgtatg gcgtactgcg cccgctcgat ttaatgcagc
    12841 cttatcgtct ggaaatgggg atccgtcttg agaatgcccg agggaaagat ctgtatcaat
    12901 tctggggaga tatcatcacc aacaagctga acgaggcgct cgcagcacaa ggcgataatg
    12961 tggtgattaa tctggcgtca gatgagtatt ttaaatcggt gaagccgaag aaattgaatg
    13021 ccgagattat caagccggtg ttcctcgatg agaagaacgg caaatttaag ataatcagct
    13081 tctacgctaa gaaagcacgc ggtctgatga gtcgttttat tattgaaaat cggctgacca
    13141 aaccagagca actgactggt tttaatagcg aaggttactt ctttgatgaa gcttcctcca
    13201 gcaatggcga actggtgttt aaacgctacg agcagcgtta agtttgcagt caatgccgga
    13261 tgcggcgtga acgccttatc cggcctacaa aagcatgcag attcaatata ttgcagaatt
    13321 cacgtaggcc tgataagacg cgccagcgtc gcatcaggcg ctgaatgccg gattgttacc
    13381 cggcatttgt catttagcgg tgatgtttgc ctggaccatg accgccgtga tgatcatgat
    13441 gagctttctt atggtgacgc ggcggtggcg gcggtccgtg tgggtgccag cgattgcctc
    13501 gccattcata atgttgtttc caccagccgt ggtcgcgcca gtgaccgcca tcccagtaat
    13561 aaccgcgatt atcacgatcg cctatctgta attttactga cgggactaac gtaatttccg
    13621 cagcctgcgc tgccatggga gcgaccagaa ccagggaaag tgcgagtacg atagattgca
    13681 tctttttcac aggttactcc ttttatcatt gcccgttatg ggtcgatatc cggagattac
    13741 gcgatgagaa cggcggttgt tattctccgc aatcctaatc tctaagttac cgcatttatt
    13801 gagaatttct cctttttccc tgtcgtttct ctccatattt tcttcacaaa aaagccgggc
    13861 tgcataaaag caaacccggc ctgattgaga taatgaatag atgttactga tgattcatca
    13921 tcaatttacg caacgcagca aaatcggcgg gcagattatg tgaaagcaag ggtaaatcag
    13981 cacgttctgc cagctctttt ggcagatcca acgtttcacc gagaatcgct tccacgctct
    14041 ctttaaattt cgccggatgc gcggtgccga ggaacaagcc atattcgcct ggattcaact
    14101 ggtcacgcag cgcacgatac gctacggcag cgtgcggctc cgaggtgtag cccagttctt
    14161 ttaactcacg cattgtctgt tgcgtggttt catcatccac ggctgcataa cccagctctt
    14221 tcagttgcca gattttgcgg cggaacaact cttccacacg cggccagttg ttcggctgac
    14281 tcacgtccat cgcgttggat aacgtcgcct gagtcgcttt gggggaccac tgaccgtcgt
    14341 gcaggaaacg tggcacggta tcgttcacgt tggtcgcagc aataaaacgt ttcaccggca
    14401 gaccgagtga cttcgccagc agacccgccg tcaaatcgcc gaagtttccg cttggtaccg
    14461 aaacaaccag ctggttgcgc gcctcctgcg gcagctgcgc aacagcttca aagtagtagc
    14521 aaatctgcgc cagcaaacgg ctgatgttaa tcgagttagc tgagtttaac cccagcgcca
    14581 ctttcagttc ttcatcatca aacgcctgct tcaccagcgc ctgacaggca tcgaaatcgc
    14641 cgtcgatggc aacagtttcg atattgccgc ccaatgtaca gaacagtttt tcttgcagtg
    14701 gactgatttt gcctcgtgga tagaggataa ccactttcac attcggcaaa ccgtagaaag
    14761 catgagccac tgccgctccg gtatcaccgg aggtcgcggt cagaatggtc actggcttat
    14821 cacccgcaat atgggtcagc atttgtgcca taaagcgacc gccgaaatct ttaaatgcca
    14881 gcgttggccc gtggaacaat tccagacaac cgacatcgct ttcaacattg gcgaccggag
    14941 ccgggaaggc aaacgccgcg cgcacgcgct cttccaggat ttcctgcggg atttcatcac
    15001 caataaacgc cgagaggatc ttcgcactgc gggtgacaaa atccagcttc agcatctcat
    15061 caatttcagt caggctgaat tccggcaggt catgcggaaa aaacagcccc tgatttttgc
    15121 ccaacccctg ggttacggct tgcgcaaagc tgacctgctc gttgtgatct ttcagattgt
    15181 agagtttcat ttagttttcc agtactcgtg cgcccgccgt atccagccgg caaatatgaa
    15241 caaaaccttc ctgattttgc aggtagttct tacccaacca gtcggcaacg cgctgggcgg
    15301 tatccggctt gtcacacaga gcgaacaagg tcgggccgga gccggagata ccgctcgcta
    15361 ccgcgccgat ttccgcgacc gcctgccgcg cctgccggaa gcctggcagt aatcgttcac
    15421 ggtagggttc agcgataaca tctttcatca gcttcgcggc aagctcaggc tgacgggaat
    15481 agcaggcgtg aatgaagcct gccagatgtc gcccgtgcgc aatgcaatcc tggcggcgat
    15541 actgcgccgg taaaatagcc cgggcttctg ccgtcgagac tttaatcccc ggatacgcca
    15601 gcacccacag ccactcatca aacccaggca cttgctggct gatgatgtcg ttttcttcga
    15661 tcatcaactg cataccacct agaaaacacg gtgccacgtt gtcgtaatga atgctgccgg
    15721 agatacgtcc ttccagctcg cccatcaaag ccagcaaacg agtgtcatta agcggcttgc
    15781 cgcagtgttc attcatcgcc atcagcgccg cgaccaccga acaggcgctg gagcctaagc
    15841 ccgaaccgat cggcatattc ttttccagag tcatcgccac tggaatttgc ttgccaagct
    15901 cctggcaaaa acgctcccag cactgataaa cgatattttc ccgtggttct gacggcagct
    15961 tatcggcaaa gcgcccgagg ttgttgagac tgaatatctc tgccgcctca accgtaacta
    16021 catctccgag caatgcacca tcaacgggtg tcaccgccgc cccgagcaca tcaaacccga
    16081 cgctcatatt ggcactggaa gccggggcat aaactttaac catgtcagac tcctaacttc
    16141 catgagaggg tacgcagcag atcggcaaag acaccagcag cggtaacgtc attgcccgca
    16201 ccatatccgc gcagcaccaa cggcagcggc tgataatagt ggctatagaa ggccagggcg
    16261 ttttcgccat ttttcacttt gaacagcgga tcattaccat ccacttcggc aatcttcacg
    16321 cgacagacgc catcttcatc aatattgcca acatagcgca aaacttttcc ttcatcacgg
    16381 gccttcgcca cgcgcgcggc aaagcgatcg tcgagctgtg acagattcgc cataaaagcg
    16441 gcaacatcac cctcggcgtt aaactctgcg ggcagcacag gttcaatttc aatatccgcc
    16501 agctccagtt cacgtcccgt ttcacgggcg agaatcaata gtttacgcgc cacatccata
    16561 ccagaaagat catctcgcgg atccggttcg gtataaccca tttcccgtgc cagcgtggtc
    16621 gcctcggaga aactcatgcc ttcgtctaac ttgccgaaga tataagaaag cgatcctgaa
    16681 agaatgccgg agaacttcat caattcatca ccagcattga gcagattttg caggttttca
    16741 ataaccggta atccagcccc aacgttggtg tcatagagga atttccgccg cgatttttcc
    16801 gccgcatgac gcaacagatg gtagtaatcc atcgacgagg tgttggcctt tttgttcggc
    16861 gtgacaacgt ggaaaccttc gcgcaggaag tcggcatatt gatccgccac tgcctggctg
    16921 gaggtgcagt caacaatgac cgggttcagc agatgatatt ctttcacgag gcgaattaag
    16981 cgcccgagat taaacggctc tttggcttgc gccagttctt cctgccagtt ttccagattt
    17041 aggccatgca cattggtgag cagtgccttc gagttggcaa caccgcagac acgtaagtcg
    17101 atatgtttat tcttcagcca gctttgttga cgcttcagtt gctccagcag cgcaccgcca
    17161 acgccaccga cgccaatcac aaacacttcg ataacctgat cggtattgaa cagcatctga
    17221 tgagtaacgc gcacgccagt ggtcgcatca tcgttattta ccacgacaga gattgagcgt
    17281 tcagaagatc cctgagcaat agcgacaatg ttgatattgg cgcgggccag cgcggcaaag
    17341 aatttcgccg agatcccacg caaggtacgc ataccatcac ctaccaccga gataatggcc
    17401 agccgttccg tcaccgccag cggctccagt aagccttctt tcagttccag gtagaactct
    17461 tcctgcattg cccgttcagc ccgcacacag tcgctttgcg gaacgcagaa actgatactg
    17521 tattcggaag atgattgcgt aatcagcacc acggaaatac gggcgcgtga catcgcggca
    17581 aagacgcgcg ccgccatgcc gaccattcct ttcatccccg ggccggaaac gctgaacatt
    17641 gccatgttat tcagattgga aatgcccttg accggtaatt cgtcttcatc acgactggca
    17701 ccaatgagcg tacctggtgc ttgaggattt ccggtatttt taatcaggca agggatctgg
    17761 aactgggcga tgggggtaat ggtgcggggg tgaagaactt tagcgccgaa gtaggaaagc
    17821 tccatcgctt cctggtagga catcgacttc aacaacctcg catcgggcac ctgacgcggg
    17881 tcgcaggtat agaccccgtc aacgtccgtc caaatctcgc aacaatcggc gcgtaaacag
    17941 gcagccagca ccgccgcgga gtagtcggaa ccgttgcgtc caagtaccac cagttcgcct
    18001 ttttcattac cggcggtgaa gcctgccatc agcaccatgt gatcagccgg aatacgactt
    18061 gccgcaatac ggcgggtgga ctcagcaata tcgacggtag attcgaggta atgccccact
    18121 gccagtagtt tttcgaccgg atcgataacg gtaacgttgt gaccacgcgc ttctaacacg
    18181 ccggccataa tggcgatcga cattttctcg ccacggcaaa tcagcgcagc gttaatgcta
    18241 tccgggcact gccctaacaa actaatgcca tgcaggacat gttttatttg ggcaaattcc
    18301 tggtcgacga aagttttcaa ttgcgccagc gggaatcccg gctgggcggc ggcgagtccc
    18361 gtcaaaagtt cggcaaaaat acgttcggca tcgctgatat tgggtaaagc atcctggccg
    18421 ctaatggttt tttcaatcat cgccaccagg tggttggtga ttttggcggg ggcagagagg
    18481 acggtggcca cctgcccctg cctggcattg ctttccagaa tatcggcaac ccgcagaaaa
    18541 cgttctgcat ttgccactga tgtaccgccg aacttcaaca ctcgcatggt tgttacctcg
    18601 ttacctttgg tcgaaaaaaa aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc
    18661 tgtacgcgtc agcccgcacc gttacctgtg gtaatggtga tggtggtgat aatggtggtg
    18721 ctaatgcgtt tcatggatgt tgtgtactct gtaattttta tctgtctgtg cgctatgcct
    18781 atattggtta aagtatttag tgacctaagt caataaaatt ttaatttact cacggcaggt
    18841 aaccagttca gaagctgctg tcagagactc tttttttaat ccacacagag acatattgcc
    18901 cattgcagtc agaatgaaaa gctgaaaaat acttactaag gcgtttttta tttggtgata
    18961 tttttttcaa tatcatgcag caaacggtgc aacattgccg tgtctcgttg ctctaaaagc
    19021 cccaggcgtt gctgtaacca gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc
    19081 agcaatgcca tgactcgttc gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca
    19141 cttttcgtcg gttgttgtat taatgttgct aattgatagc aatagaccat cactgcctgc
    19201 cccagattga gcgaaggata atccgccacc atcggcacac cagtaagaac gtcagccaac
    19261 gctaactctt cgttagtcaa cccggaatct tcgcgaccaa acaccagcgc ggcatggctc
    19321 atccatgaag atttttcctc taacagcggc accagttcaa ctggcgtggc gtagtaatga
    19381 tatttcgccc gactgcgcgc agtggtggcg acagtgaaat cgacatcgtg taacgattca
    19441 accagtgtcg ggaaaacttt aatattatca ataatatcac cagatccatg tgcgacccag
    19501 cgagtggctg gctccagatg tgcctgacta tcgacaatcc gcagttcgct aaaccccatc
    19561 gttttcattg cccgcgccgc tgccccaata ttttctgctc tggcgggtgc gaccagaata
    19621 atcgttatac gcatattgcc actcttcttg accaaatgac cgcgaacagg gtgatcactg
    19681 tcaacttatt acgcggtgcg aatttacaaa tccttaacgt aagtcgcaga aaaagccctt
    19741 tacttagctt aaaaaaggct aaactatttc ctgactgtac taacggttga attgttaaaa
    19801 aatgctacat atccttctgt ttacttagga taattttata aaaaataaat ctcgacaatt
    19861 ggattcacca cgtttattag ttgtatgatg caactagttg gattattaaa ataatgtgac
    19921 gaaagctagc atttagatac gatgatttca tcaaactgtt aacgtgctac aattgaactt
    19981 gatatatgtc aacgaagcgt agttttattg ggtgtccggc ccctcttagc ctgttatgtt
    20041 gctgttaaaa tggttaggat gacagccgtt tttgacactg tcgggtcctg agggaaagta
    20101 cccacgacca agctaatgat gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta
    20161 cgtactttag tcatgttacg ccgatcatgt taatttgcag catgcatcag gcaggtcagg
    20221 gacttttgta cttcctgttt cgatttagtt ggcaatttag gtagcaaaca tgcagacccc
    20281 gcacattctt atcgttgaag acgagttggt aacacgcaac acgttgaaaa gtattttcga
    20341 agcggaaggc tatgatgttt tcgaagcgac agatggcgcg gaaatgcatc agatcctctc
    20401 tgaatatgac atcaacctgg tgatcatgga tatcaatctg ccgggtaaga acggtcttct
    20461 gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg atgttcctga ctggccgtga
    20521 caacgaagtc gataaaattc tcggcctcga aatcggtgca gatgactaca tcaccaaacc
    20581 gttcaacccg cgtgaactga cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct
    20641 gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac aagttcaatg gttgggaact
    20701 ggacatcaac agccgttcgt tgatcggccc tgatggcgag cagtacaagc tgccgcgcag
    20761 cgagttccgc gccatgcttc acttctgtga aaacccaggc aaaattcagt cccgtgctga
    20821 actgctgaag aaaatgaccg gccgtgagct gaaaccacac gaccgtactg tagacgtgac
    20881 gatccgccgt attcgtaaac atttcgaatc tacgccggat acgccggaaa tcatcgccac
    20941 catccacggt gaaggttatc gcttctgcgg tgatctggaa gattaatcgg cgttaccacc
    21001 gtcaaaaaaa atggcgcttt ttagcgccgt ttttattttt caaccttatt tccagatacg
    21061 taattgatcg tccattgtaa cttctttact ggctttcatt ttcggcagtg aaaacgcata
    21121 ccagtcgata ttacgagtca caaacatcat gccggccagc gccaccacca gcacactggt
    21181 tcccaacaac agcgcgctat cggcagagtt gagcagtccc cacatcacac catccagcaa
    21241 caacagcgcg agggtaaaca acatgctgtt gcgccagcct ttcaataccg cctgcaaata
    21301 aataccgttc attaacgccc caatcagact ggcgattatc catgccacgg taaaaccgat
    21361 atgttcagaa agcgccagca agagcaaata aaacatcacc aatgaaagcc ccaccagcaa
    21421 atattgcatt gggtgtaaac gttgcgcggt gagcgtttca aaaacaaaga acgccataaa
    21481 agtcagtgca atcagcagaa tggcgtactt agtcgcccgg tccgttaatt ggtattgatc
    21541 ggctggcgtc gtcactgcga cgctaaacgc ggggaagttt tcccagccgg tatcattgcc
    21601 tgaagcaaaa cgctcaccga gattattagc aaaccagctg ctttgccatt gcgcctgaaa
    21661 acctgactcg ctaacttccc gtttggctgg caaaaaatca cctaaaaaac tgggatgcgg
    21721 ccagttgctg gttaaggtca tttcgctatt acgcccggca ggcaccacag aaagatcgcc
    21781 ggtaccgctt aaattcaggg ccatattcag cttcaggttc tgcttccgcc agtccccttc
    21841 gggtaaaggg atatgcacgc cctgcccgcc ttgctctaac ccggtgccgg gttcaatggt
    21901 cagcgccgtt ccgttaactt caggcgcttt caccacacca ataccacgcg catccccgac
    21961 gctaatcaca ataaagggct tgcctaagat gatatttggc gcgttgagct cgctaagacg
    22021 cgaaacatcg aaatcggctt ttagcgttaa atcactgtgc cagacctgac cggtataaat
    22081 ccctatcttg cgttcttcca cgttctgatt gccatcaacc atcaatgact caggtaacca
    22141 aaaatggata aaacttcgtt tccgctccac ggttttatcc tcttcctgca ccgtataaag
    22201 ctcggtcaca ggaatagcga taagcggccc aacgagtttt tgcggcccgc tggtactttg
    22261 gcgaatcgca tcttccacat cgctacggta atcggcacgt tcgacaatca cttgccgaat
    22321 cagcattatc ggaatcaaca acagcaatac tgcaccaaac aaggtggtca ttttccagaa
    22381 caggggggat ttcaacatag tcttctcctt tgcaatggca gcaggatacg aaggctatgt
    22441 gggaagaatt tgaagctatg tgaagtgacg gtgaagtcga agcgaggcca gcacgccacc
    22501 ttcctgcacg ttgcgcagcg tgacttcgcc gttaaacaaa cgggcgacct cactgacgaa
    22561 cgccaacccc agaccgctgc ttttttgccc atttgcacgc ggcaaagagt aaaagcgttc
    22621 aaaaatacgt gacagcgcgt agtcaggaat accactaccg gtatccagca ccttaagggc
    22681 gacgtgttcc tgatccactt cggcgcttag cgttatgcga ccgctctcgg gggtaaaatc
    22741 gatggcgtta tccagtaaat tccccagcgc ctgctccagt aacgccggtt cagcagcaac
    22801 attaacctca gtgggcataa catgtagagt gatgtttttt tctgccaact gcacggtgcg
    22861 cgcttcgctg acgcggcgaa ataatgccgc cacatcaaca acagtcagaa cgacttcctg
    22921 acgattctcc agtcttgcct ggcgtagtaa cgtttctacc aatgcctgca tacgcgcatt
    22981 ttgcgtcaga atgttgtcgg taaaacgagc caccacttcc ggtggcggac cttcgcgtaa
    23041 aatttccgcc gcgccacgaa tcgccgccag tgggcttttt agctcatggg ttaacgcata
    23101 aacatactgc tcaatatagt ttttcccttc cagcttcacg cgcatacttt ccagcgcctg
    23161 cgcaagctta cgcaactcgc tactgccgag ttcaggaaga ggaacgggct tattgtcagt
    23221 gacggaatcg gcatagcgag tgagccgggc aatggagcgg ttgatccacc aaaccatgcc
    23281 tgcgccaatc accagtgcaa tccccagcaa aatggcgctg gcccataaaa ttcgccgctc
    23341 gctacgctta atgaccggag ccatcgccgc gttcggtttg cctacgctca aaacgccaat
    23401 aagccgcgag ccgtccataa tcggcgcggc aacatacatc accgaacttt cgggatcggc
    23461 aggattttgc aacgtgctgc gcgcaccata ctgaccacgc aacgttagcc agacgtcatt
    23521 ccagcgcgaa taatcctgcc caacggcttt atttgccgaa tcgaacaata ctttaccctg
    23581 cgaatcggtc atatagacat gatattcatt gcgcactttg ttaatgccac cgatattggc
    23641 gcgaaacggg cgatgttgta gctgattaaa cgcctgcgcc agttgcccat gcgttgggtc
    23701 cccagagagc aaatcgggac gcgccagctc cgccagcaac gttgcggtat cgattaacgt
    23761 cccctccgtt gctcttcgca cgcccggttt aacttctttg acaaaaatag ccagtacgaa
    23821 ccaggccgcc accgccacca gtaaaaaata gcccagcagc aaccgcatgc cgatacgcat
    23881 tacaggcccc tcaggctata tcccatgccg cgatgagtat taatcggtga aagatcgggg
    23941 ttgatggcgc gcagcttggc acgcaacgtt ttaatgtggg tatcaatggt gcgatcgtag
    24001 gtgtcctgcg catcttccca cacgctatcc attagttgct ggcgggacca gacgcggcct
    24061 ggcgacttga gtaacgtctt cagcaataaa aactcatacc gcgtcagcgt taatggcgtg
    24121 tcaaaccagc tgatctgcgc cgcgggttca ttcaattcaa aatgaccaat acggatgacg
    24181 ggagacggcg acgagaactt cttcacccga cgcagtaagg tgcgcaccct tgcgcacact
    24241 tcgcggggtg aaaacggttt agccacatag tcgtcagcgc caatttccag cccaagcagg
    24301 cgatcgacct cttcacttcg ggccgtcagg aacagtacag gtaacgccgg atggagcgcc
    24361 agtaactggc ggcataattc aaagccgctg atatcaggca aaccaacatc gagaatcatg
    24421 acgtcgggag cctgctggcg agctttatcc agcaccggca agcctcgctc aaagacctcg
    24481 acggcaaaac cttcctgctg caacatgtag accagcgtgt cggctatccc ttgctcatct
    24541 tccactaacc agaccgtttc ccgttgcata aatcgcctct gttattgccg ccacggcatg
    24601 acaggaaccg cgctaatcgc gtttttcggc gaaccttcta caactttgtc ggagtaagcc
    24661 agataagcga gcgcgttgcg tttggcgtca taaaagcgca cgacctgtaa cgacttaaag
    24721 accagggacg tgcgtttttt gaatactacc tcgccctgag ctttgccgtt tttaatacga
    24781 tccgacagtt caatcggccc gacttgctga caagaaatag ccgcatcgga ggtatcttcc
    24841 gccagaccca atcccccttt aatacccccg gttttcgccc ggctcacata acaggtgaca
    24901 tttttcacat cgggatcgtc aaaggcttcc acaacaattt tgtgatccgg gccgatcatt
    24961 ttaaatacgg tgtcgaccga accaatctcc tctgcatgag ccaatggccc cagcattatc
    25021 aggcttaaag acaggatcaa atgcttgtat ttcatattgt taccattttt atctattaca
    25081 cgagcaatta gttcgtcacg gtaaataaca tattgattaa gaacaaaatc tcaatacttt
    25141 tatttccgtc aagccctaaa acatactcta ctaagaaaaa aacactgaat gctaaaacag
    25201 caaaaaatgc tattatccaa ttacctgatg tcaggtgctc gttgttgaaa ggatgaggat
    25261 attttatgga tcaggccggc attattcgcg accttttaat ctggctggaa ggtcatctgg
    25321 atcagcccct gtcgctcgac aatgtagcgg cgaaagcagg ttattccaag tggcacttac
    25381 agagaatgtt taaagatgtc actggccatg ctattggcgc gtatattcgt gctcgtcgtt
    25441 tgtcgaaatc ggcggtcgca ctacgcctga ctgcgcgtcc gattctggac atcgcgctgc
    25501 aataccgctt cgactctcaa cagacattta cccgcgcatt caagaagcag tttgcccaga
    25561 ctcctgcact ttaccgccgt tctcctgaat ggagcgcctt tggtattcgc ccgccgctac
    25621 gtctgggtga attcactatg ccagagcaca aatttgtcac cctggaagat acgccgctga
    25681 ttggtgttac ccagagctac tcctgttcgc tggagcaaat ctctgatttc cgccatgaaa
    25741 tgcgttatca gttctggcac gattttctcg gcaacgcgcc gaccattccg ccggtgctct
    25801 acggcctgaa tgaaacgcgt ccgagtcagg ataaagacga cgaacaagaa gtattctata
    25861 ccaccgcgtt agcccaggat caggcagatg gctatgtact gacggggcat ccggtgatgc
    25921 tgcagggcgg cgaatatgtg atgtttacct atgaaggtct gggaaccggc gtgcaggagt
    25981 ttatcctgac ggtatatgga acatgcatgc caatgcttaa cctgacgcgc cgtaaaggtc
    26041 aggatattga gcgatactac ccggcagaag atgccaaagc aggagatcgc ccaattaatc
    26101 tgcgctgtga actgctgatt ccgatccgtc gttaacgctg caactcatct aatgcagggg
    26161 cgtccagatg cgagatgtcc cctgcggttt caacaaccca gccagacgcc agccacaggc
    26221 tttcctgata atccacgcgc gaaatagaac agttacgcag acgtaagcgg cgctctgccc
    26281 atgctggtaa tccgagaatc gtactcacca ggcatcccag tgcaatacca tgactcacca
    26341 gcagcggtcg gcttccctgc ggtaagtccc ggcaagattc cagtgccgca ttgacgcgat
    26401 cgctgagctc ctgcattgac tcgccttcag gaatacgccc gtcaacggtg ccattgacca
    26461 gctgccgacg ccagttctct tcttcttcgg tcagagaatc gatatgtctt ttttccagca
    26521 cacccatgtt taattcacgc aggcgagaat caaagatgat gtcgcagccg caggcctggg
    26581 cgatgatttc cgccgtacgc cgggtgcgtc ctaaatcgct actgatgata tgcgtaatgc
    26641 caagctcttt ggcacgggtt gccacctgca tcgcctgttg ctcacctttg gcggtcagcg
    26701 ggctgtcaga ctggccctga atacgtcgct cggcgttcca ctgcgtttca ccgtggcgga
    26761 ctaggtatac ctgtaacatg ctttttttcc gtatactgct gtaacgttaa ttttctgagt
    26821 aatgctgatt atgcaccaag ttgtctgtgc gaccaccaat cccgctaaaa ttcaggccat
    26881 tctgcaggca tttcacgaga tcttcggcga aggatcctgc catattgcat ccgttgccgt
    26941 cgagagcggt gtaccggaac aaccctttgg cagtgaggaa acgcgcgctg gcgcacgaaa
    27001 tcgggtagca aatgcccgcc gtttacttcc agaggctgat ttttgggtgg cgattgaagc
    27061 tggcatcgat ggtgacagca ctttcagttg ggtggtgatt gaaaacgcca gccagcgcgg
    27121 cgaggcgcgt tcggctactt tgccgttgcc tgcggtgatt ctggaaaaag tgcgtgaagg
    27181 cgaggcgctc ggccccgtga tgtcgcgtta taccggtatt gatgagattg gtcgtaagga
    27241 aggggcgatc ggcgtgttta ccgccgggaa actcacccgc gccagcgtct atcaccaggc
    27301 ggtgatcctg gcgctgagtc cgtttcataa tgccgtgtat caggcattgt aggacggata
    27361 aggcgttcac gccgcatccg gcattcggtg cacgatgcct gatgcgacgc t
//
LOCUS       DAZGOB010000059        27373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGOB010000059 DAZGOB010000000
VERSION     DAZGOB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.059"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..329
                     /locus_tag="ACHLIF_002645"
     CDS             81..329
                     /locus_tag="ACHLIF_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9606640.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHLIF_002646"
     CDS             307..1437
                     /locus_tag="ACHLIF_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO9606641.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHLIF_002647"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHLIF_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO9606642.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHLIF_002648"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHLIF_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHLIF_002649"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHLIF_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO9606643.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHLIF_002650"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHLIF_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO9606644.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHLIF_002651"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHLIF_002651"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO9606645.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHLIF_002652"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHLIF_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO9606646.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHLIF_002653"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHLIF_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO9606647.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5871..7124
                     /gene="kch"
                     /locus_tag="ACHLIF_002654"
     CDS             5871..7124
                     /gene="kch"
                     /locus_tag="ACHLIF_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO9606648.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(7179..7352)
                     /gene="yciY"
                     /locus_tag="ACHLIF_002655"
     CDS             complement(7179..7352)
                     /gene="yciY"
                     /locus_tag="ACHLIF_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO9606649.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7495..8955
                     /gene="cls"
                     /locus_tag="ACHLIF_002656"
     CDS             7495..8955
                     /gene="cls"
                     /locus_tag="ACHLIF_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO9606650.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8990..9319
                     /gene="yciU"
                     /locus_tag="ACHLIF_002657"
     CDS             8990..9319
                     /gene="yciU"
                     /locus_tag="ACHLIF_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO9606651.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9372..10376)
                     /gene="oppF"
                     /locus_tag="ACHLIF_002658"
     CDS             complement(9372..10376)
                     /gene="oppF"
                     /locus_tag="ACHLIF_002658"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO9606652.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10373..11386)
                     /gene="oppD"
                     /locus_tag="ACHLIF_002659"
     CDS             complement(10373..11386)
                     /gene="oppD"
                     /locus_tag="ACHLIF_002659"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO9606653.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11398..12306)
                     /gene="oppC"
                     /locus_tag="ACHLIF_002660"
     CDS             complement(11398..12306)
                     /gene="oppC"
                     /locus_tag="ACHLIF_002660"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO9606654.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12321..13241)
                     /gene="oppB"
                     /locus_tag="ACHLIF_002661"
     CDS             complement(12321..13241)
                     /gene="oppB"
                     /locus_tag="ACHLIF_002661"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO9606655.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEEKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13327..14958)
                     /gene="oppA"
                     /locus_tag="ACHLIF_002662"
     CDS             complement(13327..14958)
                     /gene="oppA"
                     /locus_tag="ACHLIF_002662"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO9606656.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            15242..15538
                     /locus_tag="ACHLIF_002663"
     CDS             15242..15538
                     /locus_tag="ACHLIF_002663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606657.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(15696..16343)
                     /gene="ychE"
                     /locus_tag="ACHLIF_002664"
     CDS             complement(15696..16343)
                     /gene="ychE"
                     /locus_tag="ACHLIF_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO9606658.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(16374..16427)
                     /gene="ychE"
                     /locus_tag="ACHLIF_002665"
     CDS             complement(16374..16427)
                     /gene="ychE"
                     /locus_tag="ACHLIF_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO9606659.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            16820..19495
                     /gene="adhE"
                     /locus_tag="ACHLIF_002666"
                     /gene_synonym="adhC"
     CDS             16820..19495
                     /gene="adhE"
                     /locus_tag="ACHLIF_002666"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO9606660.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            19619..20515
                     /locus_tag="ACHLIF_002667"
                     /pseudo
     CDS             19619..20515
                     /locus_tag="ACHLIF_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(20797..21414)
                     /gene="tdk"
                     /locus_tag="ACHLIF_002668"
     CDS             complement(20797..21414)
                     /gene="tdk"
                     /locus_tag="ACHLIF_002668"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO9606661.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            22019..22432
                     /gene="hns"
                     /locus_tag="ACHLIF_002669"
     CDS             22019..22432
                     /gene="hns"
                     /locus_tag="ACHLIF_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO9606662.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLASVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(22576..23484)
                     /gene="galU"
                     /locus_tag="ACHLIF_002670"
     CDS             complement(22576..23484)
                     /gene="galU"
                     /locus_tag="ACHLIF_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO9606663.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(23686..24699)
                     /gene="rssB"
                     /locus_tag="ACHLIF_002671"
     CDS             complement(23686..24699)
                     /gene="rssB"
                     /locus_tag="ACHLIF_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO9606664.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(24791..25696)
                     /gene="rssA"
                     /locus_tag="ACHLIF_002672"
     CDS             complement(24791..25696)
                     /gene="rssA"
                     /locus_tag="ACHLIF_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO9606665.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            25809..26267
                     /gene="ychJ"
                     /locus_tag="ACHLIF_002673"
     CDS             25809..26267
                     /gene="ychJ"
                     /locus_tag="ACHLIF_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO9606666.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            26317..27159
                     /gene="purU"
                     /locus_tag="ACHLIF_002674"
     CDS             26317..27159
                     /gene="purU"
                     /locus_tag="ACHLIF_002674"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO9606667.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     SDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
BASE COUNT         7182 a         6714 c         6353 g         7124 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata ccggccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagaaaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaatg tgatttaaat
     2281 caatttttaa atattttctc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgcccaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccattttact ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct
     3001 cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttct gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caagatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctaaat ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg cgaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ccgtacttga tgtggggcgc gcgggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gatatcgcgt tttggctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc
     4801 ttcttttggc ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 tggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttaattta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat
     5581 tcaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg
     5641 gtcagtgcat tcaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt
     5701 gaacatattc aggaaaatgc cctttcctga cgcagagttg ctcgctcaaa acctttgatt
     5761 actgccgata aagtcatcta ctattgttta acacattcac gcgctgcatg tatgttcaga
     5821 aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca gtgagtcact
     5881 gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac gacattctcg
     5941 cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt atgtcggtca
     6001 atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc ttgctggcaa
     6061 acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc ttactgttcc
     6121 gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta atttacaccc
     6181 tgcattttta tccctggctg aaatttagta ttggattttg catttttacg ctggtgtttt
     6241 tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca atttttgcat
     6301 ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat ttaagcgaag
     6361 gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata gaaaccatgt
     6421 caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg ttcactattt
     6481 cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt tttggtccgc
     6541 ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg catcgtaaag
     6601 atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg caactgaatc
     6661 aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc aagcaacttg
     6721 agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac agttcagtat
     6781 taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat aacgatgcag
     6841 ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa acagttctcg
     6901 ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat atcattctct
     6961 caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa gagattaata
     7021 atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc gataacgatg
     7081 aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta aagacgaaag
     7141 agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta gatcgcaaac
     7201 aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat acgcatattt
     7261 cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg ctgcatgcgc
     7321 cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa taacagaatg
     7381 gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt atttgccaga
     7441 tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta atctatgaca
     7501 accgtttata cgttggtgag ttggttggcc attctgggat actggttgct cattgcaggc
     7561 gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc ctggctgttg
     7621 attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg cgagctccat
     7681 ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc aaaatggctt
     7741 aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt cgctgcgcca
     7801 ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa tcagctacaa
     7861 ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca gctcgcgcgc
     7921 cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga ccaggtggct
     7981 gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct cgactccgcc
     8041 gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc cggtattgaa
     8101 gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat ggacctgcgc
     8161 caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag catgaatatg
     8221 gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga tctgatggcg
     8281 cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga ctgggagatt
     8341 gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc gtttgaacag
     8401 gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga agatctcatt
     8461 caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat gaccacgccc
     8521 tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca gcgcggggtg
     8581 gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg ggccagtcgc
     8641 gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga aggcgggtta
     8701 ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac cgttaacctt
     8761 gatatgcgga gtctgtggct gaatttcgag attaccctgg caatcgacga taaaggtttt
     8821 ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct gctcgatgcc
     8881 cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt ttacttcttc
     8941 agtccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta tggatatgga
     9001 tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata tttttctcga
     9061 actggctgcc gacaatctcg atccagctga tgttctgctg ttcaatcttc agtttgaaga
     9121 gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg ttgatttcga
     9181 tttgaaccca gacttttttg ccgaagtggt gattggtctg gcggacagtg aagatggtga
     9241 aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca aactttgcca
     9301 tatcatctgg cgggaataaa taatcgcttt tagcgtgaaa aagggctgac aactgtcagc
     9361 ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct gaaactcccc
     9421 tccagaacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaacg ggtacggaaa
     9481 acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa ctggatggtt
     9541 ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc cctggtgtaa
     9601 ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc atggccgaga
     9661 tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg agcgataaaa
     9721 attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac ctgcgcctga
     9781 attgacacgt ccagcgccga aaccggctca tcgcagataa tcagcttcgg ttcaagaata
     9841 agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg cggatagcgg
     9901 ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg ctcgcgaact
     9961 tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat ctcgccgatg
    10021 gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat atcactgcga
    10081 acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca ggcaacatga
    10141 ccgtcggtcg ccttgaccaa accgatgatg gcgcgagcaa aggtggactt accgcatccc
    10201 gattccccta ccacacctaa tgtttcccct tcatacaggc gaagagtgac accatcgacg
    10261 gctttgagcg ttttcggcgg ttgccagaac cactgtttgc catctttgat ttcaaagtgc
    10321 actttaagat cggcgatttc aaggaggact tttcttcctt cagttacagc attcataaca
    10381 gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct tccagcggcg
    10441 gtgcgctact acaaatttcc atcgcatgtg gacaacgtgg ctggaacggg caacctttcg
    10501 gtaatcgcag caagtttggc ggattacccg ggatggtcaa cattgtttca ccttccgcat
    10561 caagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt tgataaaaga
    10621 catcgcgcgc attgccatat tccatcgtgc gcccggcgta cattaccagc actttgtcac
    10681 agatccccgc caccacgcca agatcgtggg tgatcataat gatggcggta ttaaattccc
    10741 gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc agcgcagtag
    10801 tgggttcatc cgcaatcagt agcttaggtc gacatagcaa tgccatcgca atcatgactc
    10861 gctgacgcat gccgccagaa aattcgtgcg ggtacatttt catgcgttta cgcgcttccg
    10921 gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct ttgctcatgt
    10981 tcttatgcag catcagcact tccattaact gctcaccgac gcgcatatag ggattcaacg
    11041 aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta ttgagttcat
    11101 gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca atacgtccgt
    11161 tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa ccagactcac
    11221 ctacaatacc cagcgtttct ccggcacgta gggaaaaatt caaatcattg accgccgtga
    11281 cgtcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc agtgcgtcag
    11341 cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct gcactcctta
    11401 acgatctttc gggtcgaggg catcacgcaa gccatcgccg ataaagttga aacaaaacag
    11461 cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt ccatcgagtt
    11521 cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac ccaaccccag
    11581 gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg cgtagaccac
    11641 caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg tcgatacacc
    11701 gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt gcccacgcac
    11761 aatacgagcc atatccagcc aggaaaccat gcctatcgcc acgaaaatca gcaggatgtt
    11821 ttgaccgaaa aaggtcacca gcaaaatgac gaagaacatg aatgggaagg agttgaggat
    11881 ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg aaagcgaacc
    11941 ataaagtgtc ccgacgacca cagccaccag tgccgcagca acaccgacca tgagtgagat
    12001 acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag taccaaagta
    12061 gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat cgtcataggc
    12121 aaactgggaa agcatcggtg ccaggattac aaataacgcg atcagcacca gcactatcag
    12181 actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc acaagctgcg
    12241 cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt tcttacttaa
    12301 catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc atatagcaca
    12361 tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag gcttaacact
    12421 aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc cggcaaacca
    12481 taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg gcccatataa
    12541 gagagcacgg gtaacagagc aggttttaat gcgtgacgta aaatgatccg ccgcataggt
    12601 aaccctttcg cccgggcagt acgaataaaa ttggagtgta atacttcaat catagagcca
    12661 cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat cggcaatatc
    12721 atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa aatgatcgca
    12781 aatatcatga ctaataatgg tgcaaccaca aaactgggga taacaacccc ggtcattgcc
    12841 agccccatca cggtatagtc ccatttggtg ttttgtttta atgcggcaat aacgccagca
    12901 ctaaccccca gtattaccgc aaggaaaaat gctgcggctc ccagtttggc agaaacggga
    12961 aaactggatg caaccaagtc attgaccgaa taatctttat atttaaacga tggaccgaaa
    13021 tcaccgtgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg atcattaaga
    13081 tgatatttct cttcgatatt ggccatcact tctggcggta aagtacgttc gccggtaaaa
    13141 gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat aaatagcgtc
    13201 ggaatcgctt ccagacagcg acgtagaata aattttaaca ttgccctacc ttctggcctg
    13261 tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct gccccacgta
    13321 ttgccattag tgcttcacaa tgtacatatt ccgggtatag gtattatcca gcggatcttt
    13381 gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt aataaacagg
    13441 aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag ctgtgcgctg
    13501 cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg ggctcttata
    13561 atgcgcggta ttcatcgagc tgttcgaaag catggtgttc aggaaggaag ttggttcgtt
    13621 gtagtcagca caccagcctg cacgggccac atcaaaagta ccctggtgac gggtgtcgag
    13681 gaacgttttc cactcctggt taaccagttt gacgtttaca ccaatgtttt tcttccacaa
    13741 tgaagaggca gcaatcgcca gctttttatg cagatcggag gtgttataca acaggttgat
    13801 ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg cttcttcgtt
    13861 acgtttttcc tggctccagc caaaccattc cggctgagtc aattttgcgc catcagtata
    13921 cggtggagtg taaccatagg cgggcatgtc gccctgcgct ttcactttat taacaatgat
    13981 atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga atggcggttt
    14041 ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa cttcgtccgg
    14101 gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttataag tcatgtcgat
    14161 ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca aataggttac
    14221 ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa gaacgattcg
    14281 ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat taccaggctg
    14341 ggtccatttt tcgccgaatt tctcgatagc ggcttttggc accggtgaag ttgatgggtg
    14401 aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta atgtgtgatc
    14461 atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa tttcatcaat
    14521 accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt tcggatcaac
    14581 agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgccat cagaccattt
    14641 cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc aggattcagc
    14701 gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa acaggtctcg
    14761 gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact gaacttctga
    14821 accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg gtacatcagc
    14881 tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta ctaaacttct
    14941 cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg tattaaccag
    15001 catgtgtaat cccggattgg gaccctgcac aggctcggag actttctgct gtcaggttac
    15061 tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag acgatcacct
    15121 tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc gacgctcttt
    15181 aattgttaat tattctcatt accaataaat aatcgtctgg cggttgcccg gaaagtacca
    15241 aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct cttttattga
    15301 ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa ttcaatcaaa
    15361 tagagattgg ctaagcatgt ctatcttttc tgattacagt tctagtagtg aaatgcacaa
    15421 taatttaaca attgattatt atttagcact ttcctctacc aaaggcagtg gaattactaa
    15481 tatcatttca attattcttc agcaagccca ggattatgat gtggcgaaaa taacatgatg
    15541 gtgaggagaa gcagttaagg aaatccttat aactaactgt aatttgagaa gaatattttt
    15601 acctattcct tttgcttttg ataactgcgc gggaacaaag taaaaataat gacgaagtaa
    15661 acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc ggggaaaatc
    15721 cccttaatac cagtaacgat aaattcaatc cccaatgcca tcagcaatag ccccataata
    15781 cgcgtaatca cgttgatgcc ggtctggcgt aaaacccgta ccagccacgg tgccatgcgg
    15841 aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc aaacagatag
    15901 ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc tggccccgcc
    15961 atcaacggca acgccaatgg caccacacca atgctttcac gtaccgcggt ttctgatttt
    16021 tcttgcttgt tctgtttatc ctcgccaagc ttgccgctga tcatcgacat cgctatcgtc
    16081 accaccagga tgcccccggc gatacggaac gaatcaattg atataccaaa aagttgcaga
    16141 atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag gttggctgta
    16201 aggttagttt tgtttcgcgc tgccgctgtc tgataactgg tcatgctgat aaagacggga
    16261 ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa gtaaacggga
    16321 aaatcaaaaa aggtctgaat cacggttagc tccgaagcaa aagccggata atgttagcca
    16381 taaataaggt tgaaaagacg cgctgacaat acgccttttg acagcatttt tcacctccta
    16441 actacttaaa attgctatca ttcgttattg ttatctagtt gtgcaaaaca tgctaatgta
    16501 gccaccaaat catactacaa tttattaact gttagctata atggcgaaaa gcgatgctga
    16561 aaggtgtcag ctttgcaaaa atttgatttg gatcacgtaa tcggtaccca gaagtgagta
    16621 atcttgctta cgccacctgg aagtgacgca ttagagataa taactctaat gtttaaactc
    16681 ttttagtaaa tcacagtgag tgtgagcgcg agtaagcttt tgattttcat aggttaagca
    16741 aatcatcacc gcactgacta tactctcgta ttcgagcaga tgatttacta aaaaagttta
    16801 acattatcag gagagcatta tggctgttac taatgtcgct gaacttaacg cactcgtaga
    16861 gcgtgtaaaa aaagcccagc gtgaatatgc cagtttcact caagagcaag tagacaaaat
    16921 cttccgcgcc gccgctctgg ctgctgcaga tgctcgaatc ccactcgcga aaatggccgt
    16981 tgccgaatcc ggcatgggta tcgtcgaaga taaagtgatc aaaaaccact ttgcttctga
    17041 atatatctac aacgcctata aagatgaaaa aacctgtggt gttctgtctg aagacgacac
    17101 ttttggtacc atcactatcg ctgaaccaat cggtattatt tgcggtatcg ttccgaccac
    17161 taacccgact tcaactgcta tcttcaaatc gctgatcagt ctgaagaccc gtaacgccat
    17221 tatcttctcc ccgcacccgc gtgcaaaaga tgccaccaac aaagcggctg atatcgttct
    17281 gcaggctgct atcgctgccg gtgctccgaa agatctgatc ggctggatcg atcaaccttc
    17341 tgttgaactg tctaacgcac tgatgcacca cccagacatc aacctgatcc tcgcgactgg
    17401 tggtccgggc atggttaaag ccgcatacag ctccggtaaa ccagctatcg gtgtaggcgc
    17461 gggcaacact ccagttgtta tcgatgaaac tgctgatatc aaacgtgcag ttgcatctgt
    17521 actgatgtcc aaaaccttcg acaacggcgt aatctgtgct tctgaacagt ctgttgttgt
    17581 tgttgactct gtttatgacg ctgtacgtga acgttttgca acccacggcg gctatctgtt
    17641 gcagggtaaa gagctgaaag ctgttcagga tgttatcctg aaaaacggtg cgctgaacgc
    17701 ggctatcgtt ggtcagccag cctataaaat tgctgaactg gcaggcttct ctgtaccaga
    17761 aaacaccaag attctgatcg gtgaagtgac cgttgttgat gaaagcgaac cgttcgcaca
    17821 tgaaaaactg tccccgactc tggcaatgta ccgcgctaaa gatttcgaag acgcggtaga
    17881 aaaagcagag aaactggttg ctatgggcgg tatcggtcat acctcttgcc tgtacactga
    17941 ccaggataac caaccggctc gcgtttctta cttcggtcag aaaatgaaaa cggctcgtat
    18001 cctgattaac accccagcgt ctcagggtgg tatcggtgac ctgtataact tcaaactcgc
    18061 accttccctg actctgggtt gtggttcttg gggtggtaac tccatctctg aaaacgttgg
    18121 tccgaaacac ctgatcaaca agaaaaccgt tgctaagcga gctgaaaaca tgttgtggca
    18181 caaacttccg aaatctatct acttccgccg tggctccctg ccaatcgcgc tggatgaagt
    18241 gattactgat ggccacaaac gtgcgctcat cgtgactgac cgcttcctgt tcaacaatgg
    18301 ttatgctgat cagatcactt ccgtactgaa agcagcaggc gttgaaactg aagtcttctt
    18361 cgaagtagaa gcggacccga ccctgagcat cgttcgtaaa ggtgcagaac tggcaaactc
    18421 cttcaaacca gacgtgatta tcgcgctggg tggtggttcc ccgatggacg ccgcgaagat
    18481 catgtgggtt atgtacgaac atccggaaac tcacttcgaa gagctggcgc tgcgctttat
    18541 ggatatccgt aaacgtatct acaagttccc gaaaatgggc gtgaaagcga aaatgatcgc
    18601 tgtcaccacc acttctggta caggttctga agtcactccg tttgcggttg taactgacga
    18661 cgctactggt cagaaatatc cgctggcaga ctatgcgctg actccggata tggcgattgt
    18721 cgacgccaac ctggttatgg acatgccgaa gtccctgtgt gctttcggtg gtctggacgc
    18781 agtaactcac gccatggaag cttatgtttc tgtactggca tctgagttct ctgatggtca
    18841 ggctctgcag gcactgaaac tgctgaaaga atatctgcca gcgtcctacc acgaagggtc
    18901 taaaaatccg gtagcgcgtg aacgtgttca cagtgcagcg actatcgcgg gtatcgcgtt
    18961 tgcgaacgcc ttcctgggtg tatgtcactc aatggcgcac aaactgggtt cccagttcca
    19021 tattccgcac ggtctggcaa acgccctgct gatttgtaac gttattcgct acaatgcgaa
    19081 cgacaacccg accaagcaga ctgcattcag ccagtatgac cgtccgcagg ctcgccgtcg
    19141 ttatgctgaa attgccgacc acttgggtct gagcgcaccg ggcgaccgta ctgctgctaa
    19201 gatcgagaaa ctgctggcat ggctggaaac gctgaaggct gaactgggta ttccgaaatc
    19261 tatccgtgaa gctggcgttc aggaagcaga cttcctggcg aacgtggata aactgtctga
    19321 agatgcattc gatgaccagt gcaccggcgc taacccgcgt tacccgctga tctccgagct
    19381 gaaacagatt ctgctggata cctactacgg tcgtgattat gtagaaggtg aaactgcagc
    19441 gaagaaagaa gctgctccgg ctaaagctga gaaaaaagcg aaaaaatccg cttaatcagt
    19501 agcgctgtct ggcaacataa acggcccctt ctgggcaatg ccgatcagtt aaggattagt
    19561 tgaccgatcc ttaaactgag gcactataac ggcttccaca acagggagcc gttttcttat
    19621 gccacttctt aatgatctgc tcgatttcag tgaccatccg cttatgcctc cgccctctgc
    19681 acaactattt gcagaacacc ttcccaccga gtggatacaa cactgcctga cgctttctgc
    19741 tcatgcgacc gttcgccgcc gtcgtttacc gggggacatg gttatctgga tggtggtgca
    19801 atgagccaat taccgatgtt gttcgccgtc tgaacctgag cgcggatggc gaagcgggga
    19861 tgaacctgct ggcccgcagc gctgtcaccc aggcgcgtca gcgcgtgggg gccgccccag
    19921 tggaatggct cttccgccag accgcacaga caggggcgcg gaacgttacc tgaaggatga
    19981 ctggcacggc ctgcaacttt ttgccattga tggcgcacag ttcaggacac ctgataaacc
    20041 cgagctgcgt gaatattatg ggtctgccaa cacatccact aagcggcaga acgcctaccc
    20101 ggttatgcgt ctggtggcct taatgaacct gggaagtcac attctgctga atgccgtgac
    20161 cgcaccttac cggcagagcg aaactgtgct ggcccactcc atgctcgcca ccatcccgga
    20221 taactctatt acgttgtttg ataaactttt ttacagcgaa gacctgctgc tgacgctgaa
    20281 ccagaagggt tgtaaccgcc actggctact gcctgcgtgg aagaatatcg catcagaaat
    20341 gatagagctg ggaaacacgg cgtcaacagg gaccatcccg aaacgactgg agcatcttcg
    20401 gggtgctctg gaagtggtgt tcataacaaa acgcccccga ccatcaaggc cgaggtcggt
    20461 taagatatca aaaacccgtt atcctgtgaa acatagtgcc gctcctctta agtgaacggc
    20521 attgcccttc tggggccgta ttttttgttt acccaaagca acttttccat aaaccgacag
    20581 cattagcctt catcatattt gcgacgatgt ataacgccta aacacaggga tattgtactt
    20641 tacaggtcac aagtcaacgt cggtgcttaa gagccctgtg aggcgtatag cggcgttaaa
    20701 aaactgccga gaagggtata tagcccggaa gaagtgcgta aaacgaactg acaggataaa
    20761 agtgccctgc tcaccctgtc agtaaagaaa ttcttattaa tcgtggcgat gcctttcctg
    20821 aatagccgtt aatgagccga cttgtaacgc ctctttatag tgtttacggc atacagaaac
    20881 gtatcgttca ttaccaccaa ttaccacctg ctcaccttcg ttataaggtc tgcctgcttg
    20941 atcaagacgc agcaccatgc ttgctttacg gccacaaaaa cagatggttt ttaattcaac
    21001 cagtttgtcg gaccatgcca gtaagtattg gctgccaata aataattcac ctcgaaaatc
    21061 ggtacgtaaa ccataacaaa gtacgggtat atcgagttga tcgacaacct ccgataattc
    21121 atatacttgt tgtctggtta aaaactggca ttcatcaacc agtacgcaat gaattgcctg
    21181 ctgttcatgt tccgcacgaa tctcatcaaa taatgatgaa ttttggttaa ataattttgc
    21241 aggcgatgac aaacctatac gcgaactgac tttcccggca ccaaagcgat catcaatttc
    21301 tgccgtatat acgacagtgc gcatgccgcg ttcctggtaa ttgtatgaag attgcaacaa
    21361 tgctgtagac ttacccgcat tcattgcgga atagtagaaa tatagctgtg ccatcagcca
    21421 caggccctca atgatatgaa ataaagttgg ctggagttta tcataattcg tcagtttttc
    21481 agctcaacag tatgcacaga agtgggtata tgcgttctcc cttacgaagc cttgcataat
    21541 ccttctgagc tatcattaca actgcctcgc ttgttataag cgggtaaatg actgctggta
    21601 actattcaca atctttaacc tgttgcgcaa gtaatagccc tctgttgacc tccaggagat
    21661 agtgcaatac taagtccatg ctcttattgc gactgttcta cttttcatca ttcgcttaat
    21721 agggaattct cgtaaacaca actaatacag aagactgaaa ggtcgtcagc ctacgataat
    21781 ctccccataa aatgtgacat gaatcaggaa gttttaacct cacgtgctgc gaaatcatcg
    21841 gtgtaaatag ggctatatgc cgcgtctttt ctggctaatt ttatgaaaag atatttattg
    21901 gcggcacaaa ataaagaaca attttgaatt ccttacattc ctggctattg cacaactgaa
    21961 tttaaggctc tattattacc tcaacaaacc accccaatat aagtttgaga ttactacaat
    22021 gagcgaagca cttaaaattc tgaacaacat ccgtactctt cgtgcgcagg caagagaatg
    22081 tacacttgaa acgctggaag aaatgctgga aaaattagaa gttgttgtta acgaacgtcg
    22141 cgaagaagaa agcgcggctg ctgctgaagt tgaagagcgc actcgtaaac tgcagcaata
    22201 tcgcgaaatg ctgatcgctg acggtattga cccgaacgaa ctgctgaata gccttgcttc
    22261 cgttaaatct ggcaccaaag ctaaacgtgc tcagcgtccg gcaaaatata gctacgttga
    22321 cgaaaacggc gaaactaaaa cctggactgg ccaaggccgt actccagctg taatcaaaaa
    22381 agcaatggat gagcaaggta aatccctcga cgatttcctg atcaagcaat aatcttttgt
    22441 agattgcact tgcttaaaat cccgccagcg gcgggatttt ttattgtccg gtttaagaca
    22501 atttaataag aataagctat aaaaaaacgg cgtcgattgc tcaacgccgt ttcgtggata
    22561 acaccgatac ggatgttact tcttaatgcc catctcttct tcaagccagg ctttaaattc
    22621 cgtgccaagg gtgttatgac gaataccgta ttcaacgaag gcctgcatgt aacctaattt
    22681 attaccgcag tcatggctct tccctttcat atgataggct tccaccgttt ctttttcaat
    22741 cagcatatca attgcgtcgg tgagctgaat ttcatcacca gctcccggag gggtttttgc
    22801 cagcaacggc caaatatccg cgctaagtac gtaacgaccc acaatagcga gattagacgg
    22861 cgcaacatcc gctttcggtt tttctaccac accaaccatc ggtacgcttt cacccggcgc
    22921 taattcaacg cctttgcaat ccacaacgcc atatgcggtc acatcagcaa ccggttcaac
    22981 catgatctgg ctatgacccg tttcatcaaa gcggcggatc atctctgcca ggttatcctg
    23041 tgacaaatcg gattcatatt catccagaat aacatcaggc aaaataacag ctaccggttc
    23101 atcacccact accgggtgag cacacaatac cgcgtgtccc aggcctttcg ccagaccctg
    23161 acgaacttgc ataatagtca cgtgcggtgg acaaatagac tgcacttcat caagcagttg
    23221 acgttttaca cgtttttcca gcattgcttc cagttcaaaa ctggtatcaa agtggttttc
    23281 aatagagttt ttagatgagt gtgtaaccag cacaatttca gtaatgccag ccgcaataca
    23341 ttcattcacg acgtattgaa ttaatggctt atcgacaagt ggcagcatct ctttcgggat
    23401 ggctttcgtc gccggcaaca tcctggttcc taatcccgca acggggataa cggctttttt
    23461 gactttcgta ttaatggcag ccatttaaat tctcctggac tgttcgtgtt ttgaacgtgt
    23521 tcatatttct gtatcgcatc ccagtatatc agcaccccga aggggctaag ttctgaaaag
    23581 gaggcgagtt agcataatta agacaattac ttatacgaat ggcgacgata gtaccaccag
    23641 ccgacattag caggtaatgc aaatttagcc cgcgttatcg tttgctcatt ctgcagacaa
    23701 catcaagcgc agtcgaccac cggttcccca tatttggcat tgccaggcat cgcatcgctg
    23761 gctcaattga ttcaaataag cgttacctaa agtgcctaac ggaacaccat tactgatttg
    23821 cacctggtgt tcgccggtat ttaacgtcgc attcagaccc gcagaaacca gaatgagatt
    23881 tttcagttcg cgatgataat agccaactaa tagcggaaac tgccccggca gattggcctg
    23941 acgaagtaaa tggtttacct gcttcaataa cgcgcccaac tctggcaacc gttgattttg
    24001 gtgtgcaagc tgttcctgta ataatccgtt aaacaatgcg cgtaataaca aggcagcaag
    24061 tacgccatta tgtccagctc gggtgacatc aaggcaataa aatgccagat cgttttccga
    24121 aagtgcggca atatcaagca ccaggccggg tttgtccgcg gcaaccaatt gacgataatt
    24181 aacccggcaa tgggaaatca cctgctgaac cggcggttgt agttcctgta ataatttcgc
    24241 cgctgcggca gggttatcaa ccattgcatc ccagtcgcga aaaagccttt cctcttcctc
    24301 aacgcgcgaa ttaaacatgc tgggatagag acaggcaaaa accatctcgc gcaagcgatt
    24361 caaatcttta actggtttca gcaaaacatc ttcaacgccc agacgtaacg ctttggcaat
    24421 atctgccata ttttcggtgg cagatatcac cagaaccggt gtctggtcac ctctgttgcg
    24481 tatatgttcc agcagtttaa gcccgttcat tcgtggcatc gcgatatcac atatcatcag
    24541 gtctggagtg aaacctccca gcaactcaag ggcatccacc ccatcagccg ccagtaccgt
    24601 tgtcgctccc aatgaggaaa accacgaatc cagaagcgag cgaaataccg gctcatcttc
    24661 aacaatgaga atctgttttc cgaccaatgg ctgcgtcatg ttctctcccc tgactggctt
    24721 tactcaatag tggcatgcta ttgccacctg cgcctgtcag aattaactta agtgtagata
    24781 aaaattctgg tcaaatgttg gtgcgtacca acggcaaaag ttcgtccatt ttcttttcca
    24841 ctgccagctg tccggccgca atggcagcgt gcgcgcgatg gaaatcaagc gtagatattt
    24901 gcgggcaaac aggttgaatc agaatatcgg gcggatcacc tgccatgcgg ttccttttaa
    24961 ggcggttctc cagcacctgg atagaagtgg tcataatctc tgttgccgtt ggcgctgtca
    25021 ccgcccgacg tgtcgttatg ctgcctaacc tttctttcag acgcgcatgc cacggcagag
    25081 aatcaccatt ctcgctattt tcttcactga cattaaagga gagcaaatct tgttgcatca
    25141 aatgagcatc gtgctgcagg tcaaccgcta tcacaatatc agcccccaat gcacgcgtga
    25201 gggaaatagg aattgggtta acgactgctc catcaaccag ccagtagccg ttatgtgcaa
    25261 caggtgccat gagtcctgga atactgcatg atgcgcgaat agcaagatgg agatcgcctt
    25321 cagtaaacca taattcacgt cccgtactta aattggtggc aacagccgca aagcgacggg
    25381 aacaattttc gatctctgtt tccggcatta tttcgcggta ttgattgaag acacgctcgc
    25441 cgcgcagtaa cccaccgcgc tgccaggaga gatccatcag gcgtaaaaca tcccaataac
    25501 tgaaagaggt cacccaatct tccagcgcag ataatcgatc gcatgcatag gcagcgccca
    25561 ccagcgaacc aattgaacat cctgcaacga tatcaatttc aatacccact ttttttagcg
    25621 cattaataac gccaatatgc gaccaacctc tcgccgcgcc agatcccagc gccagcccta
    25681 tttttatctt tctcattatt cccgcaagat tcccctggaa tgcaatcgta gccacatcgc
    25741 tactgctcgg ttaacatagt gctactcagg cggttaatac gccgttattg tttccaggga
    25801 aattatttgt gtctcagctt tgtccctgtg gtagtgctgt cgagtatagc ctatgttgcc
    25861 acccttatgt gtctggtgaa aaggttgcac ctgatccaga acatctcatg cgttcgcgtt
    25921 actgcgcttt tgtgatgcaa gacgcagatt atttaataaa gacctggcat ccctcttgtg
    25981 gagcagcggc gttacgtgcc gaattgatgg ccggatttgc gcatactgaa tggttgggat
    26041 tgaccgtatt tgaacattgt tggcaggacg ccgacaatat cggatttgtc agttttgttg
    26101 ctcgatttac cgaaggtggt aaaacaggtg caataattga acgttcgcga tttttgaaag
    26161 aaaacggtca atggtactat attgacggta cacgtccgca gtttggtcgc aacgatccct
    26221 gcccttgtgg ttcaggtaaa aaatttaaaa agtgctgcgg ccaataatgg ttgacggtac
    26281 ggtttagcaa acactctcaa caaggttttt ccagcaatgc attcactcca acgtaaagtt
    26341 ctgcgtacta tttgtccgga ccaaaaaggt ctgatcgcac gtattaccaa tatttgctac
    26401 aagcacgagt taaatatcgt acagaacaat gaatttgttg atcaccgtac cgggcgcttt
    26461 tttatgcgca cggaactgga agggattttt aatgattcca ccctgctggc ggatctcgat
    26521 agcgcattgc cagaaggctc cgtgcgtgag ctgaatcctg ccggtcgtcg ccggatagtg
    26581 attctggtca ctaaagaagc gcattgcctt ggcgatttgt tgatgaaagc caattacggc
    26641 ggcctggatg tcgaaatcgc ggcagttatt ggtaaccacg atactttacg ttctctggtt
    26701 gagcgttttg atattccgtt tgagctggta agccatgaag ggttaagccg caacgagcac
    26761 gatcaaaaga tggcggacgc cattgatgct tatcaatctg actatgtggt gctggcgaag
    26821 tatatgcggg tattaacgcc ggaatttgtg gcacgcttcc cgaataaaat catcaatatt
    26881 caccattcat tcctgcctgc gtttatcggc gcacgtcctt atcaccaggc ctatgaacgt
    26941 ggtgtgaaga ttattggcgc aaccgctcac tatgtgaatg acaatctgga cgaaggccca
    27001 atcatcatgc aggacgttat tcatgtcgat catacctaca cagctgaaga tatgatgcgc
    27061 gcaggtcgtg acgtcgagaa aaacgtctta agtcgtgcgc tatacaaagt actggcacag
    27121 cgcgtctttg tttacggtaa tcggacgatt attctttaat cgccagcaaa aataactggt
    27181 tacctttaat ccgttacgga tgaaaattac gcaaccagtt catttttctc aacgtaacac
    27241 tttacagcgg cgcgtcattt gatatgatgc gccccgcttc ccgataaggg agcaggccag
    27301 taaaaagcat taccccgtgg tggggttccc gagcggccaa agggagcaga ctgtaaatct
    27361 gccgtcatcg act
//
LOCUS       DAZGOB010000060        27372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGOB010000060 DAZGOB010000000
VERSION     DAZGOB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.060"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(66..455)
                     /gene="mutT"
                     /locus_tag="ACHLIF_002675"
     CDS             complement(66..455)
                     /gene="mutT"
                     /locus_tag="ACHLIF_002675"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO9606668.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(515..3220)
                     /gene="secA"
                     /locus_tag="ACHLIF_002676"
     CDS             complement(515..3220)
                     /gene="secA"
                     /locus_tag="ACHLIF_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO9606669.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3282..3794)
                     /gene="secM"
                     /locus_tag="ACHLIF_002677"
     CDS             complement(3282..3794)
                     /gene="secM"
                     /locus_tag="ACHLIF_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO9606670.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(4025..4942)
                     /gene="lpxC"
                     /locus_tag="ACHLIF_002678"
     CDS             complement(4025..4942)
                     /gene="lpxC"
                     /locus_tag="ACHLIF_002678"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO9606671.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5043..6194)
                     /gene="ftsZ"
                     /locus_tag="ACHLIF_002679"
     CDS             complement(5043..6194)
                     /gene="ftsZ"
                     /locus_tag="ACHLIF_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO9606672.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6255..7517)
                     /gene="ftsA"
                     /locus_tag="ACHLIF_002680"
     CDS             complement(6255..7517)
                     /gene="ftsA"
                     /locus_tag="ACHLIF_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO9606673.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7514..8344)
                     /gene="ftsQ"
                     /locus_tag="ACHLIF_002681"
     CDS             complement(7514..8344)
                     /gene="ftsQ"
                     /locus_tag="ACHLIF_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO9606674.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNHAQAEQQ
                     "
     gene            complement(8346..9266)
                     /gene="ddlB"
                     /locus_tag="ACHLIF_002682"
     CDS             complement(8346..9266)
                     /gene="ddlB"
                     /locus_tag="ACHLIF_002682"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO9606675.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9259..10734)
                     /gene="murC"
                     /locus_tag="ACHLIF_002683"
     CDS             complement(9259..10734)
                     /gene="murC"
                     /locus_tag="ACHLIF_002683"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO9606676.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISAYNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10788..11855)
                     /gene="murG"
                     /locus_tag="ACHLIF_002684"
     CDS             complement(10788..11855)
                     /gene="murG"
                     /locus_tag="ACHLIF_002684"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO9606677.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11852..13096)
                     /gene="ftsW"
                     /locus_tag="ACHLIF_002685"
     CDS             complement(11852..13096)
                     /gene="ftsW"
                     /locus_tag="ACHLIF_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO9606678.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13096..14412)
                     /gene="murD"
                     /locus_tag="ACHLIF_002686"
     CDS             complement(13096..14412)
                     /gene="murD"
                     /locus_tag="ACHLIF_002686"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO9606679.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14415..15497)
                     /gene="mraY"
                     /locus_tag="ACHLIF_002687"
     CDS             complement(14415..15497)
                     /gene="mraY"
                     /locus_tag="ACHLIF_002687"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO9606680.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15491..16849)
                     /gene="murF"
                     /locus_tag="ACHLIF_002688"
     CDS             complement(15491..16849)
                     /gene="murF"
                     /locus_tag="ACHLIF_002688"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO9606681.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16846..18333)
                     /gene="murE"
                     /locus_tag="ACHLIF_002689"
     CDS             complement(16846..18333)
                     /gene="murE"
                     /locus_tag="ACHLIF_002689"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO9606682.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVTAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18320..20086)
                     /gene="ftsI"
                     /locus_tag="ACHLIF_002690"
     CDS             complement(18320..20086)
                     /gene="ftsI"
                     /locus_tag="ACHLIF_002690"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO9606683.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20102..20467)
                     /gene="ftsL"
                     /locus_tag="ACHLIF_002691"
     CDS             complement(20102..20467)
                     /gene="ftsL"
                     /locus_tag="ACHLIF_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO9606684.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20464..21405)
                     /gene="rsmH"
                     /locus_tag="ACHLIF_002692"
     CDS             complement(20464..21405)
                     /gene="rsmH"
                     /locus_tag="ACHLIF_002692"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO9606685.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21407..21865)
                     /gene="mraZ"
                     /locus_tag="ACHLIF_002693"
     CDS             complement(21407..21865)
                     /gene="mraZ"
                     /locus_tag="ACHLIF_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO9606686.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22467..23471)
                     /gene="cra"
                     /locus_tag="ACHLIF_002694"
     CDS             complement(22467..23471)
                     /gene="cra"
                     /locus_tag="ACHLIF_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO9606687.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23553..23639)
                     /locus_tag="ACHLIF_002695"
     CDS             complement(23553..23639)
                     /locus_tag="ACHLIF_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO9606688.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23651..24142)
                     /gene="ilvN"
                     /locus_tag="ACHLIF_002696"
     CDS             complement(23651..24142)
                     /gene="ilvN"
                     /locus_tag="ACHLIF_002696"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO9606689.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24145..25869)
                     /gene="ilvI"
                     /locus_tag="ACHLIF_002697"
     CDS             complement(24145..25869)
                     /gene="ilvI"
                     /locus_tag="ACHLIF_002697"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO9606690.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25988..26032
                     /locus_tag="ACHLIF_002698"
     CDS             25988..26032
                     /locus_tag="ACHLIF_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606691.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(26187..27131)
                     /gene="leuO"
                     /locus_tag="ACHLIF_002699"
     CDS             complement(26187..27131)
                     /gene="leuO"
                     /locus_tag="ACHLIF_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO9606692.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         6544 a         7795 c         6569 g         6464 t
ORIGIN      
        1 cgcggacagc gtcgcatcag gcatcttgtg caaatgtcgg atgcggcgtg aacgccttat
       61 ccgacctaca gacgcttaag cttcgcaatt accggttcat tggctggcgg aaaatcatcg
      121 gcattaagac cgactagcga catccactca ccgggttgcc cttctttacc ccacggctcc
      181 ccttcccagc tttcgaccag ccagaaccac agagttatat gcctgtccgg gaattcatat
      241 tccagttttt caaatagcga aaaatgttgt ggggtaatcc cgacttcttc ctgaagttca
      301 cgcaccaccg cctgttccgg cgtttcgccc atttcaattt taccgccggg aaactccagt
      361 ttattcgcca tgtgcgcatc tgctgcgcga cgcgttataa agatttcatt gttctcgttg
      421 cgaataatac ctaccgcaat ttgcagcttt ttcattgtct taaacctata aaaaaggcgc
      481 agaatcctgc gccttttact tcaactgtta gcttttattg caggcgacca tggcactgct
      541 tgtatttttt accagaaccg cacgggcaag gatcgttacg tcctactttg cgctcaccgg
      601 tttgcgccgc cagtgcagct gcggctgcag agtcgtcatc ctgatggcta agctgctgca
      661 tttgcgctaa acgctcggct tccatacgac gctgttgttc cagctcctca acctcttcag
      721 gcatacgtac ctggactttg ctcagcgtgc tgataacttc atattttaac gactccagca
      781 tcgctgcaaa catggagaac gattcacgtt tgtattcctg cttcggatct ttctgtgcat
      841 agccacgcag gtggataccc tgacgcagat agtccatcgc tgccaggtgc tctttccaca
      901 gggagtcaag cgtttgcagc atgacgcctt tctcgaagtg acgcatcatc tcagcaccaa
      961 ccacttcttc tttacgctga tacacttcga tggactgcgc cagaatgcgc tcacgcagcg
     1021 tctcttcatg cagttctggt tctttatcca gccactcggc aattggtaaa tcgaggtcga
     1081 aatcgttctt cagacgttcc tgcagccccg gaatatccca catttcttcc agcgactgtg
     1141 gcggaatgta ggcatcaatg gtggctttga acacatcttc acgaatgctg ttgatggttt
     1201 cgctcacatc gctgacatcc agcagttcgt tacgctggga gtaaatggcg cgacgctgat
     1261 cgttagccac gtcatcatat tccagcagtt gcttacgaat gtcgaagtta cgactttcaa
     1321 ctttacgctg ggcgttggca atcgctttag tcacccacgg gtgttcaatg gcttcgcctg
     1381 gcttcatacc cagtttacgc atcatgccgg atactcggtc ggaagcaaaa atacgcatca
     1441 gcgcatcttc catcgacagg tagaaacggg aagaaccagc atccccctga cgaccagaac
     1501 gaccgcgcaa ctggttatcg atacgacggg attcgtgacg ctcggtaccg atgatatgca
     1561 ggccacctgc ttccagtacc gcatcgtgac gtacctgcca gtcggcttta attttttcaa
     1621 tttgctctac ggtcggattt tccagcgcgg caacttctgc ctgccagcta ccaccgagca
     1681 caatatctgt accacgaccc gccatattgg tcgcgatagt caccgcagcc ggataacctg
     1741 cctgagcaac aatcgccgct tcgttggcgt ggaatttggc gttcaggacg ttgtgcttaa
     1801 taccggcttt ggtcagttcg tttgacacca gctccgattt ttcgatggag atagtaccca
     1861 ccagcaccgg ctggcctttc gcagtacgtt ctttgatatc ttcaatgatc gcctgaattt
     1921 tttccgcttc agtcatgtag accaggtccg gcagatcttt acgaatcatt ggacggttgg
     1981 tcggaacaac gacggtatcc agcttgtaga tggagctaaa ttcgaaagct tcggtatcag
     2041 cagtaccggt catccccgcc agtttttcat acagacggaa gtagttctgg aaggtgatcg
     2101 aagccagcgt ttggttttcg ttctggatct gcacaccttc tttcgcttcc acagcctggt
     2161 gcagaccatc ggaccagcga cggccctgca tggtacgacc ggtgtgttcg tcaacgatga
     2221 taacttcacc atctttaacg atgtagtcga cgtcgcgggt aaacagcgca tgagcgcgca
     2281 gcgccgccgt tacgtggtgc atcagcatga tgttggccgg agagtacaga gactcccctt
     2341 catccatgat gccctctttc accagcagtt cttcaatcag caccagacca cgttcggtca
     2401 ggttcacctg gcgagatttt tcgtccaccg agaagtggcc ttcgccctgg aaggtttcgg
     2461 agtcttcttt ttcctgacgg atcaggtgcg gaataatttt attcacgcgt ttatacattt
     2521 ccgagctgtc ttctgccggg ccggaaatga tcagcggtgt acgcgcttca tcgatcagga
     2581 tggagtccac ttcgtccacc agcgcatagt gcagtttacg ctgtacacgt tcttcagggc
     2641 tgaacgccat attgtcgcgc aggtagtcaa agccgtattc gttgttcgta ccgtaagtga
     2701 tgtcagctgc gtaagcttcg cgctttgccg gtgctggcat gcccggcagg ttgataccga
     2761 cagtcaggcc aaggaattca aacagcggac ggttgttttc ggcgtcacgt tgcgccaggt
     2821 agtcgttgac ggtaactacg tgcacgcctt taccggttag tgcgttcagg taagcaggca
     2881 gcgttgcggt cagggtttta ccttcaccgg tacgcatttc ggcgatgcag cgttcgttaa
     2941 gaaccatacc gccgagtaac tgaacgtcga agtgacgcat accaaagacg cgcttacttg
     3001 cctcacgtac cacggcgaaa gcttccggga tcagattttc cagcacttcg cctttttcca
     3061 gacgtgcacg aaactctgcg gttttccctt tcagttcttc gtcggagagt ttttccatct
     3121 ccggttccat ggcattgatg atgttgacca ctttgcgcat ccggcgcagg gtgcgatcgt
     3181 tacgactacc gaaaacttta gttaacaatt tgattagcat aataaaatct caaacgcccc
     3241 gcgttgcgga gttaataaaa tgaagtaaag gtttattgtt gttaggtgag gcgttgaggg
     3301 ccagcacgga tgccttgcgc ctggcttatc cagacgggcg tgctgaattt tgcttgtggg
     3361 gtaaaatgcg cataatcaat gcgataaccc ttttcagacg gcgtgccttc ctgggtcagc
     3421 agcgcgctga gcgtatccag taatgcaaga tgttgcgcct gaagaggcaa agattcttca
     3481 gcaacgggca gtgtttgcgg tgccattgcg aaagaaagat gacggattac cgtgcgaatg
     3541 gcatgttgat gccagtaatc aacggaatag ttcgaattcg ggcggcgtgt gttcgcttcc
     3601 agcaaggcca attgaccaaa gttaactttg gctgaaggct cgtggttgcg ggttgtcgct
     3661 tttgcgggcg cgtttggttc ggcggcgttg ctgagcgcag gcaaacctaa actcgccgca
     3721 accatcccta ataagagatg cggccagaag tagcgtttac caaactgtcg ccagcgcgtc
     3781 agtattccac tcacgttatt gccatcccga tcgtattcaa acgtatttag cgcacaaatc
     3841 ttatcattaa agcctgcagt ccacagcacg aatgaagata agttgtgcgg aaaagtgctt
     3901 aagaaaacag cgttcgcacc gttttttata gctcttataa aatttaaaaa aaaacgactg
     3961 gttcacctgg ccggagagag tgccagattt gccagtcgaa ttttatacga cagtataaat
     4021 gtcgttatgc cagtacagct gaaggcgctt tgaaggccaa cggcagttct gcgtcgtcct
     4081 ggaaggtcac atattcccag gcttcctgtt tcgccaggac agcctgcagc agtttgttat
     4141 tcagtgcatg accggattta taagcggtaa atgcaccaat aatattgtga ccacacatga
     4201 acaagtcacc gatcgcatcg agcattttgt gacgcacaaa ttcgtcttca aaacgcaggc
     4261 cgtcttcgtt cagtacgcga taatcgtcaa caacgatggc acaatcgaag ctgccgccca
     4321 ggcacaaacc acgggactgc agatattcga tatcacgcat gaaaccgaac gtacgcgcac
     4381 ggctgatctg gcgcataaac gcatcagcgg agaagttcat cgcatagcgc tggttgctgg
     4441 aatcaatagc cggatggtta aaatcgatgg tgaaatccag cgaaaaacca ttgtacggct
     4501 taaattcagc ccacttatcg ccatcttcga cacgaacagt ctctttgatg cgaacaaatt
     4561 ttttggcgca gttcaactcg tcgataccgg cgtcaagcag caggtataca aacggagcgg
     4621 cgctgccgtc catgatcggg atttccggcg cgttaacttc gataacaatg ttatcgatgc
     4681 ccaagcccgc gagagcagca ttgaggtgct ctacggttga aatccgtaca tcatgctcgt
     4741 tgaccagaca cgtacagagc atggtatcac gcacagattt ggcatcggcc gggaaatcta
     4801 ccggtggatt caagtcggtg cgacgataga tgaccccggt gttggccggc gcagggcgta
     4861 acgtcagggt gactttcttg ccggtatgta aaccgacacc cgtcgcctga acgatacgtt
     4921 taagtgtcct ttgtttgatc atcgtattat ctcgccaaat tacctatcca accgaagtgt
     4981 actatacatt cggcgggcca gtttagcaca aagagcctcg aaacccaaat tccagtcaat
     5041 tcttaatcag cttgcttacg caggaatgct gggatatcca gataatccgg ctctttcgca
     5101 gtttgcggcg cattgtcatt cacgacttta gcaaccggct tctgctcctg ggtcagcgga
     5161 gccatcccat gctgctggta gcgatccatc actggctgct gaacctgctt attggtcacc
     5221 agagtgattt caggacgttt gtccatgccg atacctgtcg caacaacggt tacgcgcagc
     5281 tcgtcattca tatccgggtc aagagaagta ccgataacca cagtcgcgtt gtcggaagca
     5341 aatgcacgga tggtgttacc taccgtttcg aactcatcca gacgcaggtc gaagcccgcc
     5401 gtgatgttaa ccagcacgcc gcgcgcgcca gacaggtcga tatcttccag cagcggagaa
     5461 gagatagcca tttcagcagc ttcttccgca cggtcttcac cgctcgccac gccagaaccc
     5521 atcattgcgt agcccatctc agacattacg gtgcgtacgt ctgcaaagtc cacgttcatc
     5581 aaacccggac gagtaatcag ttcagcgata ccttgcacag cgcctttcag tacatcgttc
     5641 gctgcgccaa acgcatccag cagggagata ccgcggccca gaactttcag cagtttgtcg
     5701 ttcgggatag tgatcagaga gtccacatgc ttggacagtt cagtgatccc ctgctccgcg
     5761 aatgccatac gcttcttgcc ttcaaagttg aaaggcttag tgacgacagc aacggtcagg
     5821 atacccaaat cttttgccac ttcagcgacg actggtgctg cacctgtacc ggtaccacca
     5881 cccatacccg cagcaataaa gaccatgtct gcaccttcca gcgccgcacg caatgcatcg
     5941 cgatcctcat cagccgcatt gcggccaact tctggattag cgccagcgcc cagtcctttg
     6001 gtgataccgc taccgatttg aatcgtctgt ccaaccgctg ttttacgcag cgcttgtgca
     6061 tcggtattta ccgcgaagaa ttcaacacct tcaatgcgct cgcgcaccat gtgttcaaca
     6121 gcattaccgc cgccgccgcc gacgccgatg actttaatca ccgcgtcatt ggtaagttcc
     6181 attggttcaa acatagtttc tctccgattt gtgcctgtcg cctgaggccg taatcatcgt
     6241 cggcctcata aaaattaaaa ctcttttcgc agccaactat tgagtcgctt gatccacgag
     6301 ccaactgatg ctgtaacacg tttttctact tcagcttcac cgttaagatg tgactctttc
     6361 ccatagtgaa gcaatcccac cgccgtcgaa taatacggct cctgagcata atccgttaaa
     6421 ccggtaatgt tcagcggcgc gccgatacgc acttgcgtat gaaacacgcg ctgagcacag
     6481 gctgcaagac cttcgatctg cgctgcgcca ccggttaata caatgcctgc cgccaggtga
     6541 tgtttaaccc cttgttggcg aagcttttcc tgcaactgca atatctcttc gttgaccagg
     6601 ttgagcagct cggtatagcg cggctcgatc acctctgcca gggtctgacg ttgcagactc
     6661 cgcggcggac gaccacctac gctcggcact tccacgctct catcttttcc aacgatggaa
     6721 cccagcgcac aaccgtggcg aactttaatc gcttcggcgt cgcttggcgg cgtgccaaag
     6781 gcgtaagcga tatcactggt cacgacattg ccagcataag gaattacctt agtgtggcgc
     6841 aatgccccac cggtataaac ggcgatatcc attgtaccac caccgatatc gacgacgcag
     6901 acacccagtt cacgttcatc ttccgtcaat accgaataac ttgatgccag tccggcaaat
     6961 atcagttggt caactttcag cccacaacgt tcaaccgctt tgacgatgtt tttcgccata
     7021 tcgttgtgac atgtgatcag gtgcactttt gcctgcatcc gcacgcccga aagtcctacc
     7081 ggattcttga tcccttcctg atagtcaatc gcatactctt gcgggatcac atgcagcaca
     7141 cgatgctcat cgcgcacacg caccgatttc gcggtatgga cgacgttttc cacatcttct
     7201 tgcgtcactt cttcttcaga aataggcacc ataccaattt cattctggca gctgatgtgc
     7261 ttaccagaaa gcgccagata taccgaagag atctgacaat ctgccatcaa ttctgcctgg
     7321 tcaatggcgc gttgtacgca cttgaccacg gattcgaggt cgttcacccc gcctttatcc
     7381 ataccacgtg acgggcagct gcccacgcca atgatattga ccataccgtc gggcagaact
     7441 tcccctacta aagcggcaac cttcgcggta ccaatctcca gtcctactac cagttttctg
     7501 tccgtcgcct tgatcattgt tgttctgcct gtgcatgatt ttgttgctga gtagattcct
     7561 ctggcggcaa gggcgcccag cctactgccg ctccagagtc ataacgcaaa tcaacgtagc
     7621 taatccgttt gccatcggtt tgcgcctgct gctgtaaaac cggataaagt tctacaaagc
     7681 gagccaaacg tttcatcgta tcgccccggc caagattgag cttaatatcg ttattcagcg
     7741 tcaactgcca ggaacgccgc gcggtcatcg ccgcttcctt cagagtaaat ctgtcctttg
     7801 ccagcatctg ccccatttcg cgatagccct gcaacacttc attggcgctg ccttccgggc
     7861 catacagcat tggaagcacc tgcttgctgg tgcgatctgg cggcacgctg aaggtgtttc
     7921 cttccgcgtc taccatatgt tgatcattcc accgcgcaat cggcacatat tcaaccagat
     7981 gaatcttcaa ttcatcaggc cactgctttc tgacgctcac ctgcttaatc cacggcaggc
     8041 gttgttctat ttgcgtctgg atgatgttga catcttgggt cataaaggta cccggctcac
     8101 ccaatgccag gatcgactgc cggatatcgt cattacgcgt gtaatggcgt tcaccggtca
     8161 acaccagctt tgagagcggc aggcgttgcg catcttccat ccagcccaac acgacccagc
     8221 cgctcaccaa cactgtcgtt aaaacggtca gcaggaaaag gatccccgcc agacgcgttc
     8281 cattattgcg gcgagaagaa acctcttctt cgctatttcg cgtgttcaga gcagcctgcg
     8341 acatattagt ccgccagttc cagaattcgt actaccaact gcgagaagct catacctgcc
     8401 tgacgtgccg ccatcggcac caggctgtgg ctggtcatac ccggtgaggt attggcttcc
     8461 agcagataaa actgtccatc gctgtccagc ataacgtcaa tacgtcccca tcctttgcaa
     8521 cctaacgtcg tccatgcttt cagcactaat gcctgcaaat tggcctcttg tgacgcttcc
     8581 agacctgcgg ggcagaaata ctgtgtctca tcagagagat acttcgcctc ataatcatag
     8641 aaggttccgg acggttgaat acgtattgac ggtaaaattt cttcaccgag tatcgcaacc
     8701 gtgaactccg gcccacttag ccatttttca atcaatactt cttcatcgtg ctgaaatgcc
     8761 aatcttaatg catcttgtag agcattttct gctactactt ttgacattcc cacactggaa
     8821 ccttcgcggc tcggcttaac gataaccggc aaacccagag cagaaatttc tgctaactgc
     8881 ttatcgctca ggcctttttc aaactctgcg cgggttaacg ctacccacgg cgcgaccggt
     8941 aaaccggcac cttgccacag cagtttgctg cgtagtttat ccattgaaag cgcagatgcc
     9001 atcactccgc tgccggtata aggcaagccc atcagctcga gcatgccctg cagcgtacca
     9061 tcttcaccgc cgcgaccgtg tagcgcgata aacactttct gaaaacccat cgacttcagt
     9121 tgcgtcacgt cgacttcttt cgggtcgaca ggatacgcat caataccgcc ttcacgcagc
     9181 ccggctaaca ccgctgcgcc agaattcaga gaaacttccc gctcagcgga agtcccgccc
     9241 aacaggaccg cgattttatc agtcatgttg ttcttcctcc ggagtttgcg gcttcagttt
     9301 gatttcagct aaagaacggg caatttttcc gatattacca gccccctgaa cgagaatcag
     9361 gtcgttaccg gttaataccg gtgccagcat ctcggctacc tgcgcagggt ccggcaccag
     9421 aatgggatca attttcccac gtccacgaat tgtgcgacac agcgaacggc tgtccgctcc
     9481 cggaattggc gcttcgccag ccggatacac ttccagcatc aacagggtat caacctgcgt
     9541 cagcacattg gcgaaatcat catacaggtc gcgcgtacgg gtaaaacggt gcggctgaaa
     9601 cagcattacc aggtttttat ccggccagcc tgcgcgcgcc gctttaatgg tggcgtcaac
     9661 ttccgtcggg tggtggccgt agtcatcgac cagcattgcc gtaccgcttt taccattcac
     9721 tggctccagc gggaattcac cgaggaaatc aaaacggcgc ccagtcccct ggaagctctc
     9781 cagcgcacgt aaaatagcct cgtcatcaat tccctcttcc gtagcaaccg caaccgcagc
     9841 tgcggcgttc agcgcgttat gacgacctgg cgcattcagg gtgacgcgca tcggctcttt
     9901 gtcctggcgc agcagcgtaa agtgcccctg cgggccaatc tgctgataat cttctacacg
     9961 cacgtcggca tcttcgctga agccgtaagt cgtggtctga cgtcccactc gcggtaacaa
    10021 ttcgcggatc accggatcat caacacacat caccgcacga ccgtaaaacg gcaggttgtg
    10081 cagaaaatta ataaaagtct gttttaaatt ctcaaagtcg ccctggtagg tatccatgtg
    10141 gtcggcttcg atattggtga caatcgccac catcggttgc agatgcagga acgatgcatc
    10201 actctcatct gcttcggcaa tcaggtaccg accatgcccc aaacgcgcat gaacccccgc
    10261 cgcttttacc agcccgccgt taacgaaggt tgggtcgagc cccgcttctg cgtagatgct
    10321 ggaaaccatc gcggtggttg tcgttttgcc gtgcgttcct gcaatggcga tgccatgacg
    10381 aaaacgcatt aactcagcca gcatttcggc acgacggatc accggaatac gcgcttcatg
    10441 agctgcgaca atttccgggt tataggcaga aatggcgctg gaaacaacga ccacgctggc
    10501 atcacgtacg ttttccgggc gatggttgaa ataaatcgtc gctcccagat tcattaactg
    10561 ctgcgtgacc ggatttggcg ctaaatcgga accactgatc tgataacctt cattggccag
    10621 aacttcggca ataccgccca taccggcacc accgatgccg acaaaatgta tgtgccgaac
    10681 gcgacgcatt tcgggcacga tggaacgcag ttttgccaat tgttgtgtat tcattcttta
    10741 cgccattaac ttcttaaatt catacgatgc aaaaggcatc gctacaatta cgcccgggca
    10801 gcccggctca cttcatttgc cactcgctcg gtggcatccg gaatggatgc agcgcgggcg
    10861 cgttctgcca tggttaataa ggtttctcgc gaccacccgg ccagggtgtt ggcgacagca
    10921 tccacgctaa gctgtggctg ctcgataatt ttggctgcgc ccgctttttc cagcggtagc
    10981 gcattccagt attgctggcg gtctttatgt tgaaacggca caaacaacgc cggtagtcct
    11041 gccgcggcga tttcactcac cgttaaagca ccggagcggc aaacgacgac atccgcccac
    11101 gcatacgccg ccgccatatc atcaataaat tccgtcactt tatgctgcgg ttgccccgct
    11161 tcggcatacg cctgttcaac ggattgttgc gaacctttac cgctctgatg ccagatagtg
    11221 actgaatcac ccagtttcgc agcaacctgc ggcattgtct ggttaagaat gcgtgcgccc
    11281 tgggaaccac ccactaccag cacacgaacc ggaccttcac gtccagccaa acgttgctgc
    11341 ggcaacggca gcgccagtac atcggtacgc accgggttac ccaccacttc cgcattaggg
    11401 aaagcgcctg gaaacgcctg catcactttg gtggcaatct tcgccagcca tttattggtt
    11461 aagcccgcaa taccgttttg ttcatgaagt acaaccggaa tgcctaacga ccacgcggcc
    11521 agaccacctg gacctgatac atagccgccc ataccgagta ccacgtcagg tttgtacgct
    11581 ttcataatcg cccgcgcctg acgccaggcg ttgaagatac gcagcggcgc agctatcagt
    11641 gcttttatac cttttccacg cagaccagag atacgaatga aatcaatttc gatgccatgt
    11701 tttggcacta agtccgcttc catacggtcg gcagtcccca gccagcgaac ttgccaaccc
    11761 tgagccatta gatggtgcgc aaccgccagt cccgggaata catgtccacc ggttccgcct
    11821 gccatcacca ttaatcgctt tccttgacca ctcatcgtga acctcgtaca aacgcctgcg
    11881 ctttctccag acgcgtttca taatcaatac gcaacagcat catgatggct gtcgacataa
    11941 tcagtaagct cgaaccaccg taactgatca gcggcaatgt cagacctttg gtcggtaaca
    12001 tccccgccgc cgcgcctacg ttaaccagcg cctggaagct aaaccagatg ccaatagaac
    12061 aggcgagaaa accggaaaaa cggtggtcaa tttctaacgc tttgcggcca atcgacatcg
    12121 cgcgaaaagc gacgaagaat accattaaaa gtgccagcac cacaccgaca taccccagtt
    12181 cttcgccgat aatggcgaaa ataaagtcag tgtgcgcttc cggcagatac tccagttttt
    12241 gtaccgagtt acctaaacct tgtccccaaa gttcgccgcg accaaacgcc atcagcgatt
    12301 gcgttaactg atagccgctg ccaaagggat cttcccacgg gttccagaat gcggtaacac
    12361 ggcggatacg gtacggttcg gcgagtatca gcaacacaac cgctgaaatg cccataccga
    12421 taatggcaat gaactgccac aatttcgctc ccgccaggaa caacatcgcc agcgtagtca
    12481 caaacaacac caccaccgta ccaaggtctg gctgtgccag cagtaacact gccaacacca
    12541 gaatcacgcc catcggtttc aggaagccgc gcaggttatt acgtacttcg tcgcctttac
    12601 gcaccagata gttggcgata taacaaaaca gcgacagttt tgtcagctcc gcaggctgga
    12661 tacgcagcaa accgagatcg atccaacgcg atgccccttt aaccgagcta cccactacca
    12721 gcacgatcat cagcaggatg atagatccga gcagcatcgt ggcactgtag cgttgccaga
    12781 actccatcgg cagacgcagc gtaatgatcg ccagaatgaa tgccaggatc agatagacgc
    12841 catcacgctt cgcgaagaag aacggatcgt tggttaagcg ttgccctatg ggcattgacg
    12901 ccgaggtcac catgataaag ccaatcgccg caaggccgaa ggtcagccac agtaaggtgc
    12961 gatcgtacat gatcaggctg tcggtatctt tttcccgcga gcccatcacc cagcctttta
    13021 gcgccgtgga gatccagacc aggatactga atcctggcag gcgcggcatt ttcaggcgag
    13081 ggagagataa acgcatcaac ctaactcctt cgccagacgg gcaaactcat tgcctcgttg
    13141 ttcaaagttc ttgaactgat caaggctggc acaggccggg gagagcagaa ccatatcgcc
    13201 cggctgaaca cgcggagcca gcaagcgcat cgcctgttcc atagtttcgg tttgttctgc
    13261 cacttccggg cgtagcgccg ccagctgcgc accgtcacga ccaaaacagt acaaacgcac
    13321 gttgtcgcca ttcagatagc gcgccagtgg actgaagtcc gccgatttac catcgccgcc
    13381 cagcaacaaa tgcagcgtgc cgtctacctg caagccattc aacgccgctt ccgtactgcc
    13441 gacgttagtg gctttcgaat cgttaatcca gcgtacgcca ttgtgctcca gcaccacttc
    13501 aaagcgatgc ggcagaccgg tgaaggtggt taacgctttc aggctgctgg cgcgcggcaa
    13561 accagctgcg tctgccagtg ccagtgctgc cagcgcattg gtgtagttat gctgcccgga
    13621 aagtttcatc tctttcacgt tcagcacttt ctcgcctttc actcgcagcc aggtttctcc
    13681 ctgctgatga ttcaggtgat agtcacccat gttgacgcca aagctgacgc agcgttcatc
    13741 agcaccgcga atcggcattg ttaaggcatc atcagcattg acgacacaaa ctttcgcatt
    13801 ttcgtaaata cgcaattttg ccgcacggta ttgctgcaaa ccaaacggat agcggtccat
    13861 atgatcttca gtcacgttca gaatggtcgc cgccaccgcc tgtaagctgg aagtggtttc
    13921 cagctggaag ctcgacagtt ccagcacgta cagttcgcac tcagcatcaa gcagcatcaa
    13981 cgctggcagg ccaatattgc cacccacacc aacgttaacc cccgccgctt tcgccatttc
    14041 acccactagc gtggtgaccg tgcttttgcc gttagaaccg gtaatcgcca caatcggtgc
    14101 ttgtgcttcg cggcagaaca gctcgatatc gccaacgatt tcgattccgg catcagcggc
    14161 agcgcttaag gatggatgcg ccagtgcaat accgggactg gcgacaatca gatctgccgc
    14221 catcagccat tcatcattca gactgcccgt gtggcgttct acggcttcgg gtaatttatc
    14281 caggccaggc ggtgtcatac gcgtatccat aacgcgcggc gtcacaccgc gagcgaggaa
    14341 aaagtccacg caggaaagcc cagtgaggcc caggccgata atgacgacat ttttaccctg
    14401 ataatcagcc atgattaacg taccttcagc gttgccagac caatcagaac cagcatcagc
    14461 gaaataatcc agaaacgcac aatgacgcgc ggttccggcc agcctttcag ttcatagtgg
    14521 tgatgaatcg gtgccatgcg gaaaatacgt tgtccgcgca gtttaaagga gccgacctgc
    14581 aggatgacag aaagcgtttc taccacgaac acgcccccca taatcaccag caggaattcc
    14641 tgacgtagca gtacggcgat aatgcctaac gcaccaccta acgccagcga acctacatcg
    14701 cccataaaga cctgcgccgg ataggtgtta aaccacagga agcccagtcc tgccccgact
    14761 atcgccgtgc agacaatgac cagttccccg gcgtgtcgca gatacggtat gtgtaaatag
    14821 ctggcaaagt tcatgttgcc ggtcgcccac gccaccagcg caaaaccacc ggcgacaaat
    14881 acggtcggca taattgccag gccgtcgaga ccatcggtca ggtttaccgc gttgccagta
    14941 cccacaatga cgaagtaagc cagcagaatg tagaacagcc ccagctgcgg catcacatct
    15001 ttaaagaatg ggaccaccag ctgcgttgcg ggcgtgtctt tgccggcaag gtacagggcg
    15061 aaggcgatac ccagcgcaat gaccgacatc cagaaatact tccaacgagc gatcaaccct
    15121 ttggtgtctt tacgcaccac tttgcgataa tcatcaacaa agccaataac accgtaacct
    15181 accagcacca ccaacacgca ccagacgtac ggattggacg ggtaagccca cagcagtacg
    15241 gagatcacaa tcgccgtcag gatcataatc ccgcccatgg tcggcgtacc gcgcttgctg
    15301 aagtgtgatt caggaccgtc gttacgcacc acctgaccaa aggaaagttt ttgcaaatga
    15361 gcaatcatac gcgggcccat ccacaatgag atgaacagcg cggtcagcag gctgacgatg
    15421 gcgcgaaacg tcagatagga aaagacgtta aagccggaat aatatttgac caaatgttcg
    15481 gccagccaaa ctaacatgtc ccattctcct gtaaagcgcg tactacctct tccatggcgg
    15541 cactacgtga acccttaact aaaatcgtaa ttacctgttg ctcagcaatc agtgatttaa
    15601 gacgcgtaat taacgcagtt ttatcagcaa aatgttcgcc aacgccgctg gcggtgctga
    15661 tagcatggct ttgtttaccc acgcttaaca cgcggtcaat accagcagct tttgccgcct
    15721 cgcccacctg tacatggcag gcttcgcttt cagcgcccag ttccgccata tcgcccacca
    15781 ccagcacgcg gtagcccggc atttcagcca gtacctgaac agcggcggtc attgaaccga
    15841 cattggcgtt gtaggagtcg tcgagcagca actggttttc tgccagttgg atggggaaca
    15901 gacggcctgg aacagctttc agatttgcca gccccgcttt gatagcatca agcgttgcgc
    15961 ccacggacat ggagagcgca gcggctgcca gcgcattcgc aatattgtga cgccccggca
    16021 acggcagcag aacatcgacg ctaccggttg gggtttgtag ggtaaattcc gtaccgtgcg
    16081 aggtcacatg gatattggtg gcggtgaaat cgctgttggc ggcattgggt gagaaacgcc
    16141 acactttgcg tgagccaatt acgctctgcc agttcagcca gtcgttgttg tcggcgttca
    16201 taatggcgat accgttttcc ggcaggccgc taaagatttc acctttcgct ttcgcgacac
    16261 ccgcaagcga gccaaaacct tccagatgcg ccgctgccag gttgttgacc agcgcagctt
    16321 ccgggcgagt cagactcaca gtccaggcta tttcgccctg atggttcgcg ccaagttcaa
    16381 taactgcgta atcgtattcc ggcgttaagc gcaacagcgt catcggtaca ccgatgtcgt
    16441 tgttgagatt gcctgccgta taaagcgtgt tgccgcactg gcttaaaatc gccgccgtca
    16501 tctctttaac ggaggttttg ccggaggacc ccgtcagagc aaccacgcgc gccggaactt
    16561 gctggcgaac ccatgcagcc agttcaccaa acgccagacg cgtatccttg acgattaact
    16621 gcggcaggtc gatgtccagc ggacggctaa ccagtagtgc gcctgcgccg ccagctttcg
    16681 cctggtcggc aaaatcgtgg gcatcaaaac gttcgccttt cagggcaaca aacaggcagc
    16741 ccggcgtcag ttttcgggta tcagtggtta cagcatcaag ggtgatatct gcaccttgca
    16801 gttcaccgtt gagaatatcg gtaagttggc taagggttac gctaatcatg caatcacccc
    16861 cagcagacgc gccaccgtga cgcgatcgga gtagtccaga cgctgattgc caacaatctg
    16921 gtaatcctca tggcctttgc ccgcgaccag taccacatca ttctctttag cctgcataac
    16981 ggcgcaagtc accgcttcag cacggccttc catcactttg gcatgtccgg catctaacat
    17041 tcccgccaga atatcgttga tgatggcacg cggttcttcg gtacgcgggt tatcgtccgt
    17101 caccaccgcc acgtcagcaa actcttcggc aattgcgccc atcagtggac gcttaccttt
    17161 atcgcgatcg ccaccacagc caaagacgca ccacagcttg cccgcacagt gcaggcgcgc
    17221 cgcctgtaag gctttttcca gtgcatccgg cgtatgcgcg taatccacca ccaccgtcgg
    17281 tttgcctggc gcagtgaaca cttccatacg tccgcaaacc ggttgcagac gcgcggcggt
    17341 ttttaacaga tcagccagcg ggtagccaag tgccagcagt gtcgccagcg cgagcagcag
    17401 gttgctgacg ttaaaagcgc ccatcagatg gctttcaatt tcgccatcgc cccaacttga
    17461 actaaagcga atggtcgcgc cgctgtcgtg atagttcact tcggtcgctt tcaaccagcg
    17521 tccgtgacag ttcggattga tatgatcttc cattgatacc gcaaccgcgt ccggcagttt
    17581 tgccagccag cggcggccca cttcatcgtc ggcgttaaca atcgcctgac cacaatgatg
    17641 ttcagaataa agcagccatt tcgcggcttc gtagtgttcc atatcaccat gataatcaag
    17701 gtgatcgcgg cttaagttgg taaagaccga cgccgcaaat ttcaatgccg ccacacggtg
    17761 ctgtaccagc ccgtgggagg aaacttccat tgcgcaaaac gtcgcgccct gatccaccag
    17821 ccccgccagc tcatgctgaa catcgactgc cgaaccggtg gtattttctg tcgggatcac
    17881 tttccccagc agaccgttac caacggtgcc cattaccgcg ctggtttcgc caagcagttg
    17941 gctccactgc gccagcagct gggtagtcgt ggttttgccg ttggtgcccg ttacgcctac
    18001 gagacgtaaa ttgtcagagg gttcatggta aaagcggccc gccagtgcag ataaacgctc
    18061 gttgagctgg ctgagataga tgaccggtac gccgtgcatt tcacggattt caccgtcggt
    18121 cgcctcacct ttcgcctctg caataatggc agtcacacct tgcgctatcg cctgcgggat
    18181 atatcgacgc ccgtccgcct gatgacctac tacagctaca aagagatcgc ccgccgcagc
    18241 cacacggctg tcgagtgtca tctctcgcag tgctcgcgaa ggtgcgtctg gcacccacgg
    18301 agcaagaagg tcgcgcaaat tacgatctgc cacctgtccc ctcgccttga ttaatcacaa
    18361 attcattttt atcgcccgtt gtcagcgcat ccggctcgat gttcatggta cgcaatacgc
    18421 cgcccatgat ggcaccaaag accggcgctg aaacggcgcc gccgtagtat ttacccgcct
    18481 gcggatcgtt gataacaaca accagcgcga agcgcggctg actcgcaggc gcaacgcctg
    18541 cggtataagc aatatattta ttgatgtagc gaccgtccgg cccgaccttt ttcgcggtac
    18601 cggttttaat ggcgatacga tagcctttaa tcgccgcctt cacgccgccg ccgcctggta
    18661 gcgccacgct ttccatcata tgcaccacag tgcggacaat ggattccggg aagacacgtt
    18721 caccgggaac cggggggtca actttggtaa tcgacagtgg gcgataaatg ccgtagctgc
    18781 cgatagttgc gtagactcgc gctaactgta acggtgttac cattagcccg tagccgaaag
    18841 agaaggtggc cctctctatg tcagaccacc gttgtttttg aggatataag ccactgcgtt
    18901 ctccgaccaa ccccaaattg gtcgcttttc ccagtccaaa acgtgagtaa gtatctacta
    18961 acgctgagga cggcatcgct aacgccagct tggaaacacc gacgttactc gacttctgta
    19021 ataccccggt cagggttaat tcgctgtagc gtgccacgtc tttgatttcg tggccgttaa
    19081 ttcgataagg aatggtattg agtaccgagt tttcccgcac cacgccacgt tgcaacgcgg
    19141 tcattaccac catcggttta accgttgagc ccggttcaaa cacgtcggtg atggtacggt
    19201 tacgcatcgc ctctttcggc gtgccgctca gattgttagg gttgtatgac gggctgttag
    19261 ccatcgccag cacttcaccg gtgttgacat ccaccagcac ggcgctacca gattcagcct
    19321 tgttaaaggc caccgcgttg ttcagttcgc gataaaccag cgcctgcagg cgttcatcaa
    19381 tactcagcgc caggttgtgc gctgcctggc tgtcagtaga agaaatatct tcaattacgc
    19441 gaccatagcg gtctttacgc acaatgcgct cacccggctg cccggtaagc catttatcga
    19501 aactcttctc aacgccctca atcccttgac tatcgacgtt agtaaagccg atgaggtgag
    19561 cagtcacttc gccggacgga tagtaacggc gagactcttc acgcagatga atccccggca
    19621 gtttcagttt tttgatgtag tccgccatgt cagggttcac ctgacgcgcc agataaataa
    19681 agcgcccttt cgggttggcg ttaatgcggg ctgaaagctg atccagcgga atattgagcg
    19741 cgttagccag cgccttccag cggtcaccga cgctgatacc gccagcgtca tgcacttctt
    19801 tcgggtcagc ccaaatcgct tttaccggca cgctcaccgc taacgggcga ccagaacggt
    19861 cagtaatcat gccgcgggag gtggaaactt gctgaacgcg aagagaacgc atgtcgccct
    19921 ctttcaccag catatccggg gagataactt gtaaccacgc tacgcgtccg agcagaaaag
    19981 ccagcgccag gagaatacag ccgcataaca acgcaaaacg ccaactgata aagttggcat
    20041 gttcttcctg acgttttggt ttctgcgttt tcgccgctgc tttcatgcgt cgcgtttatc
    20101 cttatttttg cactacgata ttttcttgtg acggatcaac atgctgcatt tgcagctttt
    20161 ccgtggcgat cctttccacc cggctatggt cgccgagcgc attctcttca aggatcaggt
    20221 tgcgccattc aatgtctaaa gcatctcgct ccagcaccag ttgttcgcgc tgagcggtca
    20281 gtaaacgggt atggtgcgcc gtggttacca cagtcaccgc cgtcaaaata atgcaaatga
    20341 acaggcagag tggcagcttc ccaaatcgca aaagatcgtc accgataaca ccaggcaatg
    20401 catggcgctc gtggcttccc atcgatcctt taactttgct tagagcttct gtcactctgc
    20461 tgatcatgca ttcgtcctct ctgcaatacg cagaactgaa ctacgggcac gagggttctc
    20521 agccacctct tcttcgcccg gcattaactt gcctagtgct cgcagctgac ggccacccag
    20581 ttttttgagc tgctcttcag tcatcggtaa ccctgccgga acttgcggac cgcggctgtt
    20641 ttcacgcata aaacgtttca caatacggtc ttccagcgag tggaagctga tgatcgaaag
    20701 ccgcccaccc ggggccagca cgttgagcga gctttttagc gcctgctcta tctcctccag
    20761 ttcactgttt acccaaatgc gcaccgcctg gaaggtacgg gtcgcgggat gtttaaactt
    20821 atctttcacc ggcgttgcag cagccacgac ttccgccagt tctttggtgc gggtcatcgg
    20881 ctgttcgcgg ttacgctcga caatggcgcg ggcaatgcgt ttggcaaaac gctcttcacc
    20941 ataggttttc aatacccagg cgatatcggc ttcttctgcg gtttgtagcc attcagcggc
    21001 tgactgccca cgggttgggt ccatacgcat gtccagcgga ccatcgcgca taaaggaaaa
    21061 gccgcgctca gcatcatcaa gttgcggtga agagacgcca agatcgagga gaatgccgtc
    21121 gatcttgccg ataagatcgc gctcggcaac gtattcgccc agcgcggaga aaggtccgtg
    21181 gatgatggag aagcgcggat catcaatagt cttcgcaacg gcgatagcct gcgggtcgcg
    21241 atcgatcgcc agcaaacgcc cctcttcgcc aagctgcgag aggatcagac gtgagtgacc
    21301 accgcgacca aaagtcccat caatgtagat gccatcagga cggatattga ggccattaac
    21361 ggcttcatcc agcagcaccg tagtatgttt atagttttcc atcattttat agagacaagt
    21421 cctgcagtcg ctccgataag tctccggtag ccaactgctc tgcgtcgata tcttccttga
    21481 cctgttgatg ccaggttgtt tcatcccaca gctcaaactt gttgaactgt ccaaccagca
    21541 tcacttcttt tgtcagcccg gcatgttgcc gcagtactgg cgcgattaac aatcgacctg
    21601 cgccatccat ctgacattcg ctggcatgac ctaacagtag gcgctgcaca cggcgctcaa
    21661 ccgggttcat gctcgacaga cgcgataatt tttgctcgat aatttcccat tcaggtaggg
    21721 ggtaaagcag caggcacggg tgatgaatgt caatggtgca aaccatttga ccggcagcgt
    21781 tctcaagcag ctgttcccgg taacgggtag gcactgataa gcgccctttg ctgtcgagat
    21841 tgactaacgt tgctccccgg aacatgccag cctcacccct tattcccact ttgccccaca
    21901 atttcccact gaaaggagtt tacggagcgg aggaaaagct tgtcaagcca gtcacaaggc
    21961 ataccgaggt aaaaaacccg atgtttacgg ctaatatcag cgttgtttat tttacgtcca
    22021 acgtgcgcag caaattaacg ttatgaatag ctgtaagaaa aaatccctca tcgtcatcac
    22081 cgttttaaaa tgactcacta ttttccacag caaatataaa gtgtcagttt gcgacgcgag
    22141 cggcatttta ggacatatct tccccggtta acagtccctg ttgcgtctgt gtggcgcttg
    22201 atgccagcgg gtttgcgcaa aagcaaagaa aagtgcttgt taaatcggca attcatctct
    22261 gcatgtaaca aaataatgca aaaacgcgct gtcgatatca cttaattatt gtgaatttca
    22321 gctggtgagt taattaacgg gcgggtttaa aagaacttat caataatagt caggaatagt
    22381 cttatttact ttaaggatat tgatgtccat tcccgtactc tacgcgccag agggaaattc
    22441 acctggcgcg tatttttgtt cgcggcttag ctacggctga gcacaccgcg gcgatagaga
    22501 ttacgtttaa tgcgcgttaa accaggtttt ggcttacgcg gttcgtccag gcttgccagg
    22561 acaatctcca gcacacgctc tgcgacatcg cggtgacgtt gagccactgc cagcaccggg
    22621 cactgtaaga agtcgagcag ttcgttatcg ccaaaggtgg caattgccag gtcagaaggc
    22681 agtttaccat cgcgacgtag cgtgacatcc atcacccctt gcaacaacgc aaacgacgtt
    22741 gtgaacagcg cctgcggcat cggatgcgtt tccagccatt tttcgaataa ctgggcagcc
    22801 gcctcccgct catagctgtt ggcatacagg aaatgcactt cgcgcggatc atctttccag
    22861 gcagtacgga aaccttgttc acgcaggaag ctgacagaaa gctccggcag cgcgccaaga
    22921 taaagcaccg tctcggcggg aaacttacgt aactcttccg ccagcatttc ggcatcatcc
    22981 tgatcggcac caaccacgct ggtgaaatgt tcacgatcga gggcgcggtc cagcgcgaca
    23041 atcgggaacg ggtcgttagc ccagcgttga taaaaaggat gctcaggtgg caacgacgtc
    23101 gaaacaataa tggcatcaac ctgacgctgt aaaaggtgct caatgcagcg catttcgttg
    23161 tctggctgat cttctgagca ggcaatcagc agttgataac cccgttgccg cgcctggcgc
    23221 tcaagatagt tagcgatgcg ggtatagctg gtgttctcca gatcggggat cacaagacca
    23281 atagaacgtg tgcgtccagc acgaagccca gctgccacgg cgttcgggtg gtaattgtgc
    23341 tcacgcacca cagccatgac tttttcaacg gttttgtcgc tcacacggta ttgcttcgct
    23401 ttgccgttaa taacatagct tgcagtggtc cgcgacactc ccgccagccg agcgatttca
    23461 tccagtttca caattgcccc ttgcgtaaaa tgtaaaaacc atagccattg tactggtatg
    23521 ggttaaattc attaacatct aagcgcagaa aattaactgc ggcaactgct tttattccag
    23581 gttcccactg atttcgcaaa aaaagcccaa cgtgacatgt tgggctgcaa attgcgcact
    23641 gagatcattc tcaacgcatt attttatcgc cgcgcgaaag tccgaccaca ccagagcgag
    23701 caacttccac aattttcgcc acatcgcgaa tcgatgctaa aaatgcatca agcttatcgc
    23761 tggtgcctgc taattgaacg gtataaagcg agggggtgac atcgataatt tgcccacgga
    23821 atatttccgt attacgtttc acttcgtcac gcccgtaacc gctggcctga attttcacca
    23881 gcatgatttc ccgctcaaca tgcgcgccct gccccaactc actcacgcgc aagacatcga
    23941 ccagcttgtg taattgcttt tcgatctgct caagcacttt ttcatcgccc acggtctgga
    24001 tggtcatacg cgataatgtc ggatcgtcgg ttggcgcaac ggtcaggctt tcaatgttgt
    24061 agccacgctg ggaaaaaagg ccaatcacgc gggataacgc gcctgattca ttttcgagta
    24121 agactgataa tatccggcgc ataatcaggt tctctccgtt ttgcttaacc acatttcatc
    24181 cattccgccc ccgcgaatct gcatcgggta gacgtgctca ctgccatcga cggtaacatc
    24241 aacaaacacc aggcgattat tgcacacctg ttccagcgcc tcgctaagtt tgctttccag
    24301 ctcatgcgga tgagagatct ggatcccgac gtgaccatag gcttccgcca gacggacgaa
    24361 atcgggtagt gattgcatat aagattgtga atgacggccg gaatagatca tgtcctgcca
    24421 ctgtttcacc atccccagat agcggttatt gagattcacc accagtaccg gcaactcgta
    24481 ttgcaacgcg gtagacagtt cctggatgtt catctgaata ctgccgtcgc cagtgacgca
    24541 aaccacggtt tcttctggca acgccatttt gacgcccagt gccgcaggta aaccaaaacc
    24601 catcgtgccg aggccaccgg aattgatcca gcgacgcggt ttgtcgaatg gataataaag
    24661 tgcagcaaac atctggtgct gcccgacatc ggacgtcacg taagcgtctc ccttcgtcaa
    24721 ccgccaaaga gtctcgatca ccgcctgcgg tttaatcttt tcactgtgag tgtcatattt
    24781 caggcactga cgagcgcgcc actgttcaat ttgctgccac cagtcgcgga tctcatccag
    24841 tggttgatgg gcggattctt gcgacaagag ttcaagcatt tgttcgagga cctggcgagc
    24901 atcccccaca atcgggatat ccgcagtcac ggttttagaa atggaagtag gatcaatatc
    24961 gatatgcaga acagtggcat ttgggcagta ctttgccaga ttgttcgtcg ttcggtcatc
    25021 aaatcgtacc ccgacggcga aaatcacatc cgcgttatgc atcgtcatat tggcttcgta
    25081 ggtaccgtgc attcccagca tgcccagtgc ctgacgatgc gttgccggaa acgcccccag
    25141 ccccatcaat gaggaaacaa cgggcagatt caacgcctcc accgtttctt tcaactgctg
    25201 atggcagccc gccatgattg ccccaccgcc tacgtagaca accggttttt ttgccgctac
    25261 cagcgtttgc agagcgcgct taatttgccc tttatgtccg gtagtagtgg gattgtaaga
    25321 acgcatactg accgactcag gccagacata gggtaatttg ttcgccggat taagaatatc
    25381 tttcggtaaa tcaacgacca ctggtcctgg gcgaccactt gccgccagcc agaaagcctt
    25441 tttcagcacc tgcggaatgt cttccgtttg cttaaccaga aaactgtgtt taaccaccgg
    25501 tcgcgaaatc cccaccatgt cgcactcctg aaaggcatcg taacctatca acgaggtcgc
    25561 tacctgcccg gaaaggacaa ctaatggaat ggaatccata taagcggtgg cgatgccggt
    25621 aatcgcattg gtcgcccctg gacccgacgt taccagcacg acgccgactt ccccggtcgc
    25681 gcgcgccaga ccatcggcca tatgcactgc cgcctgctca tgacgaacta aaacatgatc
    25741 gataccaccc acggtatgca atgcatcgta aatatcaagg actgcgcctc cgggataacc
    25801 gaatacttgt ttaacgccct gatcgataag cgatcggacg accatctcgg ctccagacaa
    25861 catctccatg gcctgcctca ctgtttgacg gaaaaaatgt gtaaaacaca ttaaccgtcc
    25921 gatcttgctt aagcaattgg cagccagaga cataaacgca gggtaataag aataaacagg
    25981 aggaaaggtg aaaagaaacg ataaaatcct ccattacgaa ggattaaatt gataataaat
    26041 cggctgaatc ccacaactta cagcaatttc tctatttttt taattaatcc taaaacagag
    26101 agcccacaca ttcagaataa aattctacat tcaataaaaa atacagaata aaccagacat
    26161 tcatgcctga cctattctgc attcagttag cgtttgcaaa ttgagactaa ttgctcttcc
    26221 atccactgat ggcctttatc gcgcccggca gcttcatgcc aggagagata acaggttctg
    26281 ctattttgtt ttaacggcag cggtaatacc tgtaattcta aggattcagc gaactcttca
    26341 gccagccaac gcggcgcaat agcgaccaaa tgcgtttgcg acaccacgct aagtacgctc
    26401 atcattgcca tgccctgata cgcgatactg gcttgcttat ctaccgtgtc ataccaaggt
    26461 tgactaaatg acgcgaaacg atcgagcgaa accgccgcat gttgttcgtt ataaacatca
    26521 tgtttcagta acgggccctt aattgttgga tgatttttgc tggctaccag caccatttca
    26581 tctttaaata atggcacgct ggtaaattca ggacgatgga agtcttcata actaatcaca
    26641 aactccgttt cctgataacg cagctgatgt tcagtgttct gatttaatga agacttgaac
    26701 ataacatgta tatttggcgc aatctgctca atgtgattat aaatctgcga ggtcagaatg
    26761 ctgtctaacg ggctgcaaac acaaagatga aatacacgtt cactgctcgc gggttcaaaa
    26821 cctgaaccag gcaattcatt ttgtactagt tgcaatgcct gacgaactga gccaaaaagt
    26881 tgaaatgcgc gagcagtcgg ttgaatacca cggccataac gaacaaaaag ctcgtcatta
    26941 aacatcacct tcaggcgtgc aacagcgtta ctgaccgcag gttgcgacat acccagaaca
    27001 tgagcggcac gggtaatgtt ttgctcctgc atcacggcat cgaaaacggt taataagttg
    27061 agatcgacca tgcgtagctg tggcttgctt aactccgccg tctctgaatg atctgtttgt
    27121 acctctggca tacttaactc cactgtcaca cttaactccc tttcccttat tggaatgcag
    27181 agaaataatc ctgaagaata tgatttatca tgcatataaa ataagaaaaa gcaaaatgag
    27241 taaaattcgg aaaacataaa gatacgggtt ctgataaaaa cagaatccat tgccgtggaa
    27301 tgagtcattt accacagcat aataatccat aatgtttttg ttgcgaaaac aatctaatca
    27361 tgactaccgc ga
//
LOCUS       DAZGOB010000061        26695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGOB010000061 DAZGOB010000000
VERSION     DAZGOB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.061"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            68..1666
                     /gene="rtcR"
                     /locus_tag="ACHLIF_002700"
     CDS             68..1666
                     /gene="rtcR"
                     /locus_tag="ACHLIF_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO9606693.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETQVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYTRINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1648..2406)
                     /locus_tag="ACHLIF_002701"
     CDS             complement(1648..2406)
                     /locus_tag="ACHLIF_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO9606694.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2423..3253)
                     /gene="glpG"
                     /locus_tag="ACHLIF_002702"
     CDS             complement(2423..3253)
                     /gene="glpG"
                     /locus_tag="ACHLIF_002702"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO9606695.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3298..3624)
                     /gene="glpE"
                     /locus_tag="ACHLIF_002703"
     CDS             complement(3298..3624)
                     /gene="glpE"
                     /locus_tag="ACHLIF_002703"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO9606696.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3814..5319
                     /gene="glpD"
                     /locus_tag="ACHLIF_002704"
     CDS             3814..5319
                     /gene="glpD"
                     /locus_tag="ACHLIF_002704"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO9606697.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5371..5976)
                     /locus_tag="ACHLIF_002705"
     CDS             complement(5371..5976)
                     /locus_tag="ACHLIF_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606698.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     AWGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5979..6746)
                     /locus_tag="ACHLIF_002706"
     CDS             complement(5979..6746)
                     /locus_tag="ACHLIF_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606699.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6749..7459)
                     /locus_tag="ACHLIF_002707"
     CDS             complement(6749..7459)
                     /locus_tag="ACHLIF_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606700.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANYKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7475..8983)
                     /locus_tag="ACHLIF_002708"
     CDS             complement(7475..8983)
                     /locus_tag="ACHLIF_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO9606701.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMITRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9109..11556)
                     /gene="glgP"
                     /locus_tag="ACHLIF_002709"
     CDS             complement(9109..11556)
                     /gene="glgP"
                     /locus_tag="ACHLIF_002709"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO9606702.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11575..13008)
                     /gene="glgA"
                     /locus_tag="ACHLIF_002710"
     CDS             complement(11575..13008)
                     /gene="glgA"
                     /locus_tag="ACHLIF_002710"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO9606703.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(13008..14303)
                     /gene="glgC"
                     /locus_tag="ACHLIF_002711"
     CDS             complement(13008..14303)
                     /gene="glgC"
                     /locus_tag="ACHLIF_002711"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO9606704.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14321..16294)
                     /gene="glgX"
                     /locus_tag="ACHLIF_002712"
     CDS             complement(14321..16294)
                     /gene="glgX"
                     /locus_tag="ACHLIF_002712"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO9606705.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16291..18477)
                     /gene="glgB"
                     /locus_tag="ACHLIF_002713"
     CDS             complement(16291..18477)
                     /gene="glgB"
                     /locus_tag="ACHLIF_002713"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO9606706.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18750..19853)
                     /gene="asd"
                     /locus_tag="ACHLIF_002714"
     CDS             complement(18750..19853)
                     /gene="asd"
                     /locus_tag="ACHLIF_002714"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO9606707.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20045..20638
                     /gene="yhgN"
                     /locus_tag="ACHLIF_002715"
     CDS             20045..20638
                     /gene="yhgN"
                     /locus_tag="ACHLIF_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO9606708.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20695..22035)
                     /gene="gntU"
                     /locus_tag="ACHLIF_002716"
     CDS             complement(20695..22035)
                     /gene="gntU"
                     /locus_tag="ACHLIF_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO9606709.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22039..22566)
                     /gene="gntK"
                     /locus_tag="ACHLIF_002717"
     CDS             complement(22039..22566)
                     /gene="gntK"
                     /locus_tag="ACHLIF_002717"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO9606710.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22705..23700)
                     /gene="gntR"
                     /locus_tag="ACHLIF_002718"
     CDS             complement(22705..23700)
                     /gene="gntR"
                     /locus_tag="ACHLIF_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO9606711.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(23924..24619)
                     /gene="yhhW"
                     /locus_tag="ACHLIF_002719"
     CDS             complement(23924..24619)
                     /gene="yhhW"
                     /locus_tag="ACHLIF_002719"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO9606712.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24742..25779)
                     /gene="yhhX"
                     /locus_tag="ACHLIF_002720"
     CDS             complement(24742..25779)
                     /gene="yhhX"
                     /locus_tag="ACHLIF_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO9606713.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            26112..26600
                     /gene="yhhY"
                     /locus_tag="ACHLIF_002721"
     CDS             26112..26600
                     /gene="yhhY"
                     /locus_tag="ACHLIF_002721"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO9606714.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         6661 a         7148 c         6656 g         6230 t
ORIGIN      
        1 aaacggagat tttccacaaa accaattcac cgataatgaa atatcgtctt ttataaggat
       61 atctaagatg cgtaaaacag tggcttttgg ctttgtcggt accgtgctgg attatgccgg
      121 acgcggcagt cagcgctggt cgaaatggcg tccgacgctt tgtttatgcc agcaagaatc
      181 gttagtcatc gatcggctgg aattgttgca cgacacccgc tcgcgctcgc tattcgaaac
      241 gcttaaacgc gatatcgcca gcgtttcgcc agaaacacaa gtcgtgggcg ttgagattga
      301 actgcataac ccgtgggatt tcgaagaggt ttacgcctgc ctgcatgatt tcgcccgtgg
      361 ttacgagttt cagccagaaa aagaagacta tttaattcac atcaccaccg gcacccacgt
      421 cgcgcagatt tgctggtttc tgctggcgga agcgcgttac ctgcccgccc ggctgataca
      481 atcttcacct ccgcgcaaaa aagaacagcc gcgcggcgca ggtgaagtga cgattatcga
      541 tctcgattta agccgttata acgccatcgc cagccgcttt gccgaggaac gccagcaaac
      601 gcttgatttt cttaagtccg gcattgccac gcgtaacccc cacttcaacc gcatgattga
      661 gcagatcgaa aaagtggcga tcaaatcccg cgcgccgatt ctgcttaacg gtccaaccgg
      721 cgcgggcaag tcatttctgg cgcgacgcat cttcgagtta aaacaggcgc ggcatcagtt
      781 tagcggcgcg tttgtggaag tgaactgcgc caccctgcgc ggcgataccg ccatgtcgac
      841 gctgtttggt catgtaaaag gcgcgtttac cggggcgcgg gaatctcgtg aaggtttatt
      901 acgcagcgcc aacggcggaa tgttgtttct tgatgagatt ggcgaactgg gcgcagacga
      961 acaggcaatg ctgctaaaag ccattgaaga gaaaaccttt tacccgtttg gcagcgatcg
     1021 ccaggtgagc agtgattttc agcttatcgc cggaacggtg cgcgatttgc gccagctggt
     1081 tgccgaaggc aaatttcgcg aagatctgta cacgcggatc aatctctgga ccttcaccct
     1141 gccgggtcta cgccagcgcc aggaagatat tgaaccgaac ctggattatg aagtggagcg
     1201 ccacgcctca ctcactggcg acagcgtgcg ttttaacacc gaagcgcggc gcgcctggtt
     1261 ggcttttgcg acctctcccc aggcaacatg gcgcggtaac tttcgcgaac tttctgccag
     1321 cgtcacgcgg atggccacct ttgccactag cggacgcatc actctggacg tggttgaaga
     1381 tgagataaac cgtctgcgct ataactggca ggagagccgc ccctccgcgc ttacggcgtt
     1441 gctgggcgca gaggcagaaa acatcgatct cttcgaccgt atgcaactgg aacacgttat
     1501 cgctatctgc cgccaggcaa agtcgctttc cgcagccgga cgccagcttt ttgacgtttc
     1561 gcgccagggc aaagccagcg tcaatgacgc ggatcggcta cgcaaatacc tggcgcgttt
     1621 tggtctgacg tgggaagccg tgcaggatca gcacagctcc agttgaatat ggtggtccgt
     1681 cagcacctgc atcacgctta ctggcggcgg ggcgtcggtg tagacggcat ctaccatgct
     1741 gatgctgccc atattgacca tcgcgttacg gccaaatttc gagtgatcga caaccagcat
     1801 aacgtggcgc gagttctcaa taatggcgcg tttggtgcga acttcgtggt aatcgaactc
     1861 cagcagcgag ccgtcgctat cgatgccgct tatccccaga atgccgaaat caaggcggaa
     1921 ctgggagata aaatcgagcg tcgcttcgcc aatgatcccg ccatcgcggc tgcgtaattc
     1981 gccaccggcg agaataatgc ggaaatcttc ttttaccatc aacgtgttag caacattgag
     2041 attgttggtg acaatacgca aattgctgtg attaagcagc gcgtgcgcca ccgcttccgg
     2101 cgtggtgccg atatcgataa acagtgtcga gccattgggg atttgctccg ccactttgcg
     2161 ggcaatcctc tctttttctt cggtctgggt ggctttacga tcgtgccacg gcgtgttaac
     2221 cgaactggaa ggcagcgccg caccgccatg gtgacgcagg atcaggtttt gctccgccag
     2281 ctcattgagg tcgcggcgaa tagtctgcgg gctgacggag aaatgctcta ccagctcttc
     2341 agtactgaca taaccctgct gtttaaccag ttcgataata ccgttgtgac gttgtgtttg
     2401 tttcatttat aaatccctgg aattattttc gttttcgcgc attgagcgaa tcaacaaaag
     2461 ccatcgctaa acccacggct aacccggcga tgtgtgctcc gttcgccatc gacatcccaa
     2521 acaaatcaaa ccatccggcg acaatccaga tcagcgcaaa gataattaac ccacgttgca
     2581 ggtaaatgcc actttgcgga tcgcgttcgc cacgtagcca gacgtagccc atcagcgcat
     2641 acaccacgcc agaaagcccg ccaaaccacg gcccgctgaa tttttgctgc acatagccgc
     2701 ttaacagggc gctaatgaga gtaataacaa ttagcttacc gctaccgagg cgtttttcca
     2761 ccgcaccgcc gagataccac caccagagca ggttaaagag gatatgcatc agcgagaagt
     2821 gcattaacgc gtgggtgaag taacgccaga actcaaattt cagtgttgga tcgaatggcc
     2881 aggccagcca taacatcact tcctgatcgc cgagaatttg catggcgata aacaccacca
     2941 cgcaggcgat catcaccacc caggttaccg gacctgcgcg ttcacgcaag gcagcaaaga
     3001 aaggataacg gcgataatgc agaccactgc cggtatggcc cgcctgccag ctggccgcca
     3061 gataacgcgg atctgccggg ttttcgagaa aacgcgccag ctccgcccgc acgcgctcgg
     3121 cctgggactc atccgccagc cagacatcgc tttggttatg ttgttgaatc gtgaggataa
     3181 caccctgcgt cgccatataa tcaacaaacg cctgcgccac gcgggggtta gcaaaagagg
     3241 taatcatcaa catcgttgct gtcgcttatt ccacacaaaa ggggacagta taaagcgtta
     3301 cgcgccgtac gccacctctg cgggaaactg acgctgccag gcttcaaaac cgccgtcaat
     3361 gctatagacc acatcgtagc cctgttgcag cagatactgc gccgcgcctt tgctgctatt
     3421 gccgtgataa cacatcacca tcaccggagt gtcaaagtcg ttatcacgca taaacgcgcc
     3481 cagcgtgtcg ttggttaaat ggaaagcctg caccgcatgc cccatagcga aactctgtgg
     3541 atcgcgaata tcgaccagca ccgcctcttt ttcctgcaac ttctggtgcg cgtcggcaac
     3601 gttaatacat tcgaacagat ccatgcgtct ctctttcttt acaaacaagt gggcaaattt
     3661 accgcacagt ttacgtcgaa gcggcagata aacgccataa tgttatacat atcactctaa
     3721 aatgtttttt caatgttacc taaagcgcga ttctttgcta atatgttcga taacgaacat
     3781 ttataagctt taacgaaagt gaatgagggc agcatggaaa ccaaagatct gattgtgata
     3841 ggtggcggca tcaatggtgc tggtatcgcg gcagacgccg ctggacgcgg tttatccgtg
     3901 ctgatgctgg aggcgcagga tctcgcttgt gcgacctctt ccgccagttc aaaactcatt
     3961 cacggtggcc tgcgctacct tgagcactat gaattccgcc tggtcagcga ggcgctggct
     4021 gaacgtgaag tgctgctgaa aatggctccg catatcgcct tcccgatgcg ttttcgcctg
     4081 ccacatcgtc cgcatctgcg cccggcgtgg atgattcgga ttggtctgtt tatgtacgat
     4141 catctgggta aacgcaccag cctgccggga tcaaccggtt tgcgttttgg cgcaaactca
     4201 gtgttaaaac cggaaattaa gcgcggattc gaatattctg actgttgggt agacgacgcc
     4261 cgtctggtac tcgccaacgc ccagatggtg gtgcgtaaag gcggcgaagt gcttactcgg
     4321 actcgcgcca cctctgctcg ccgcgaaaac ggcctgtgga ttgtggaagc ggaagatatc
     4381 gataccggca aaaaatatag ctggcaagcg cgcggcctgg ttaacgccac cggcccgtgg
     4441 gtgaaacagt tcttcgacga ggggatgcat ctgccttcgc cttatggcat tcgcctgatc
     4501 aaaggcagcc atattgtggt gccgcgtgtg catacccaga agcaggccta cattctgcaa
     4561 aacgaagata aacgtattgt gttcgtgatc ccgtggatgg acgaattttc catcatcggc
     4621 accaccgacg tagagtataa aggcgatccg aaagcggtga agattgaaga gagtgaaatc
     4681 aactatctgc tgaaagtgta taacgcgcac tttaaaaaac agcttagccg tgacgatatc
     4741 gtctggacct actccggtgt gcgtccgctg tgtgatgatg agtccgactc gccgcaggct
     4801 attacccgtg attacaccct tgatatccat gatgaaaacg gcaaagcacc gctgctgtcg
     4861 gtattcggcg gtaagctgac cacctaccga aaactggcag aacatgcgct ggaaaaactg
     4921 acgccgtatt atcagggtat tggcccggcg tggacgaaag agagcgtgct gccgggtggt
     4981 gccattgaag gcgaccgcga cgattacgca gctcgtctgc gccgtcgcta tccattcctg
     5041 actgaatcgc tggcgcgtca ttacgcacgc acttacggca gcaacagcga gctgctgctc
     5101 ggcaatgcgg gggcgataag cgacctcggg gaggatttcg gtcatgagtt ctacgaagcg
     5161 gaactgaaat acctggtgga tcacgaatgg gtccgccgca ccgacgatgc cctttggcgt
     5221 cgcacaaaac aaggcatgtg gctgaatgcg gatcaacaat ctcgtgtgag tcagtggctg
     5281 gtggaatata cgcagcagaa gttgtcgctg gcgtcgtaag gatgatgccc ggttgaagtg
     5341 caaccgggct atttggattc cttagactat tcattgtaaa taattgaata acggcccctg
     5401 agctaaaatc gccaaactta agcacgttac ccaatcgccc cacttcttcc tctggtttct
     5461 attttttctg caccaatggc aataatatcc ccatgcaaaa ccaagctgtg cactaatcgg
     5521 gccgacaaaa tcagtccaag gctgcggcca aaaaggggaa aaattcaggg gaggccagac
     5581 aaacgtcaga aagaaatttc ggatattttc cagcaacgct gatttctctg gagaaatata
     5641 aagaatgaaa ttcgagagaa ttaaccagaa atagaccggc gaaaagacca gccagcaatg
     5701 cagtctgttt tttaattcag gaggcgttct gcgggagaat tgccaaccga tgccagccat
     5761 tacactgaaa aaaagtgcat attcaaccat taactcaagt tgtaattcct gaagagaaat
     5821 acgcacaacc aggagcggaa aggagaatat tatttctgcc agaaaagtta acgtgaatat
     5881 aagtacggct aagacaataa ccgtcggcca taccaataat ggcacgataa caaaaccgag
     5941 aattacaggg ggaatataaa gccggaattt aatcattttt attttcctgg tgattcggat
     6001 gatgcgtcat acatcgcgca taaacactca tcaattatcc agtcatcttc ccagacctga
     6061 tattctgtca ttggttcggg ctgttttagc gggttctcaa tcgcttgccg cgctctttct
     6121 ttcagtaaag gatgattatt tctagcccag gcattgattt gggtggtaat ggcgttatcg
     6181 atatcgtcct ggttatctct gatataatcc tcacaatatc tgttcaataa acaggcaaga
     6241 gaaacggtcg accaaaagcc gggaactgaa ttaatggcat ccagcaatgg cgcaagagcg
     6301 atatcggcac ggaaaaacat ttccagcata tactgtaatt cgacagcttc atcgccggat
     6361 acccaatcag tcatatactg gcaggcaaat cgctgtaaaa ttgcccgttc atccgcacgc
     6421 cagttttctt tgttggcaag gttgatacgg cgcagagaaa aaactacgtg aatattgtca
     6481 aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa aatagcggac ctcatcgcta
     6541 ttttgcggat caggccctgg gctactattc caggcattga ttaaggaata cgggatcgct
     6601 cgtaacgatg tactccgtaa tgccttttgt tcctgctgac tcaggcaata ttcacaacaa
     6661 accacaaatt tctgcggcac ccgataacga cgaaaggtgt gataaatctg ctcaattaat
     6721 gcctgcatgg cggggctggg ttgcatagtt accccctcgc ccgctgatcg cgtgtaaacg
     6781 gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat aagggcgcta cgcgaaaggg
     6841 aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct ttcctctgcg caattttcca
     6901 ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt atcgcgaaat ccgcgtaaac
     6961 tgttattgtt atgaaatttc caggcatctt taaagacctg aaagatatag acatcctcaa
     7021 tccaggagtc acatttttta agcgggcgct ttgctattat cacccgctca tccggattta
     7081 gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt caggtcacat tgaacatctg
     7141 gtacatcact gatatagggg tcaaatccaa aatcagcacc acaggcacaa agacggctaa
     7201 tggtggcggt atcgagggaa aatcccggcc ataccttgct ggaggttaag ccaatattta
     7261 atgtgacatc atatcgaagc aatccctcaa gaccgtcagc accgacgaac ttttcccctt
     7321 catccagggc ctggttaata agttgctgaa caatgccttc atcccagtat gccgatttgt
     7381 agttggcaat cgtaatatcc acgcctgtga agggatcagt accggcaagg cttgcaaata
     7441 atgtattaac taattccatt tttattatcc agctttaagg tagcgtctta tacatcggga
     7501 cataacctac atcttccacc aaactctgtc cctgcggcgt taaaaaccac tccaacaatt
     7561 tttgtgtctc tgacgtcgta ttttcccgcg ttatcataaa ggcatcgacg atatagggat
     7621 atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc gttaatcgct agcagtttta
     7681 tatttttatc ggcgttcatt tgtgttgcgt aataacggaa ggtgtaacct attgcgttgt
     7741 ttgtattacg gtattcggca acgactttaa tcattccctc catcatactt gccacttctg
     7801 tttcctgcgg cgagatcatg cggacatttt tcatgacctg ggattgcatc accgtctggc
     7861 taccagagtc ttccgggcgt tgccaggtct ggatctcctg atcgttaccg ccaacagtac
     7921 gccagttggt aattgcacca ctgaagatgt cacgcacctg ttgttcagtc agggaattaa
     7981 ccggattatc cgcattgacg atgaaaacaa acgcttcacg ggcaaaaggc gtgtacagca
     8041 aagtaacgcc cgattcctca gcgcgttttt tctgcccacc ggaaggttgc gccacaaaaa
     8101 taatatcagc atcaccttta acaatcctgt tatacgcatc gggggtacga gagctctcca
     8161 gatattccct ggtgtgaaaa tcttccggta taacgcttaa agcataaaat gcagaagcat
     8221 aaattgggta cgccgccgta gcaccatcga gtcgcggcca gttttgcgta aactgaattt
     8281 gcggtttacc acgcagtggt gtcagttgat tattgagttt atcgggtcgc caggcccagg
     8341 tatcaatatt ttcgcgcaca ccgacgccgg gattgagctg tttatataaa taaccctgat
     8401 aaccagcgac aaaaccaagc atgagaagga taaacaggag ggaattacgg caacgtagcc
     8461 cggatgtacc ggttaaagga tgtctggttg gccggccgta ccccagcgaa aaactgatat
     8521 gcgcgccaac gggtataatg actatccacc agtactcacc tccgactact gagactaaca
     8581 aaacgcccag ccaggggagc ataagatcag ccaaccaacc cgatcccatc tggtgaaatt
     8641 gataatctgc cagatgcagc gcgattatcc agccaaccag gattaataaa ccgggtacta
     8701 aaaaaagaca ttgtcgccag acggctattt ctcttttttc tggtgcacta tcgcctgccc
     8761 atactcgccc cgagaaaaat agcattaatg cgcccagcgc cagcataaaa atgttgagca
     8821 gaaggtaact tgttggcgtg ataaggatat tacaaacttc aataataccg gcaagtccga
     8881 cacccagcat agcaacaacc gccgccaaaa attgcgtcag tatggggatg ccgaaaaaag
     8941 tcattaccag cgaggacaaa atccattttc tgttttgcat tattctttcc attctttttg
     9001 aatggtgaaa ttatactccc cgagtcccct tgccccttct ggacactttt ccgaaatgat
     9061 ggcggaaaaa aacgggagcc tttggccccg ttctatttat tggtgaactt acaatctcac
     9121 cggatcgata tgccagatat gatcggcgta ctctttgata gtacggtcag aagagaagta
     9181 gcccatattg gcaatgttca gcatcgcttt tgcggtccac tcttcctgaa gctcgtagag
     9241 ttcgtcgact ttatcctgac aatcgacata gctgcgataa tccgccagaa cctgatagtg
     9301 atcgccgaag ttgatcagcg aatcaaccag atcgcgatag cgacccggat cttccggact
     9361 gaatacaccg ctgccgattt gcgtcagcac ctgatgcagc tcctcatctt tctcgtagta
     9421 ttcacgcggt ttgtagccct gacgacgcag ttcttccact tcttccgcag tgttaccaaa
     9481 gataaagata ttgtcagcac cgacatgatc cagcatctcg acgttcgcac cgtccagcgt
     9541 accgatagtc agcgcgccgt taagcgcaaa cttcatgtta ctggtgccgg aagcttccgt
     9601 ccctgccagc gaaatctgtt cagacagatc tgccgccgga atgatcaact gcgccaggct
     9661 aacgctgtag ttcgggatga acacgacttt cagcttatcg ccaatctgcg gatcgttgtt
     9721 gatcactttc gctacgtcat tgatcaaatg aataatgtgc ttcgccatgt aataggccga
     9781 agccgcctta ccgccaaaaa tattcacgcg cggtacccac ttcgcatccg ggtcggcctt
     9841 gatgcggtta tagcgggtaa tcacatgcaa cacattcatc aattgacgtt tgtattcgtg
     9901 aatacgtttg atttgtacat cgaacaacgc ctttggattc accaccacat tcagctgctg
     9961 ggcgatatac tctgccagac gctttttgtt ctccagcttc gcctgatgca cagcgtgatt
    10021 aaccattggg aaatcacagt gttgttgcag ctcattaagc aggctaaggt cggtgcgcca
    10081 gttacggccc aggtgttcgt ccagcacggc tgaaagcgat gggttcgcta ccgctagcca
    10141 gcgacgcggc gtcacaccgt tggtgacgtt ggtgaaacga cccgggaaga ttttcgcaaa
    10201 gtcggcaaac aacgattgca ccatcagatt agagtgcagt tccgatacac cgttaacttt
    10261 gtggctcaca acaaccgcca gccaggccat acgcacacga cgaccgttgg attcatcaat
    10321 gatcgacgcc cgtcccagca gatcggtatc gttcggatac tgttcctgca aggttttcag
    10381 gaaatagtcg ttgatttcaa agatgatctg caggtgacgc ggcagaattt tacccagcat
    10441 atcaaccggc caggtttcca gcgcctcgct catcagcgta tggttagtgt aggagaagac
    10501 ctgacaacac acctcaaacg cgtcgtccca gctaaattgg tgctcatcga tcagcagacg
    10561 catcatctca ggaatcgaca gtaccggatg ggtatcattg agatgaatcg cgattttatc
    10621 cgccaggtta tcgtaggttt tatgcaactg ataatggcgg cttaaaatgt cctgaatggt
    10681 cgaggaaacc aggaagtatt cctgacgcag gcgcagctca cgcccggagt aggtggagtc
    10741 atccggatac agtacgcgag atacgttctc ggagtggttt ttatcttcca ctgccgcgaa
    10801 gtagtcaccc tggttgaatt taccgaggtt aatttcgcta ctggcttgcg cactccacaa
    10861 acgcaacgtg ttggtcgcgt cggtgtcgta accagggatt atctgatcgt aagcgactcc
    10921 cagaatctct tcggtttcaa tccagcgcgt ttttttacct tcctgctgaa tgcgaccacc
    10981 aaaacggact ttatagcgcg tgttgtggcg tttgaattcc cacgggttac cgtattccag
    11041 ccagtagtct ggcgactctt tctggctacc gttaacgatg ttctgcttga acataccgta
    11101 gtcatagcgg atgccgtaac cgcgccccgg caaccctaac gtcgccagag aatcaaggaa
    11161 gcaagccgcc agacgtccca ggccaccgtt accgaggcct gggtcatttt cttcatcaat
    11221 cagctcttcg agatttaacc ccatcgcttc cagtgcgccc tgtacatctt cgtaaattcc
    11281 tagcgacaac atggcgttgg agagcgtacg gccaatcaaa aactccatcg acaggtagta
    11341 aacctgacga gtttcttgcg acaactgggc acggtttgaa cgtaaccagc gctccacgag
    11401 acgatcgcgc acagcaaata acgttgcgtt cagccattca tgtttattgg cgacgaccgg
    11461 gtcctttcca atcgtaaaca tcagcttgta agcgatagag tgcttaagag cttctacgct
    11521 aagcgtgggc gatgaatatg taaacggagc attcatatag gcgtttcctg aaaactattt
    11581 caagcgatag taaagctcac ggtacgactt cgccgcgacc tgccagctaa aatccattgc
    11641 catagcctga cgttgcacaa accgccacag tgaaggacgg gaccacagta caaaagcacg
    11701 tcgaatagcc cgtaacagcg accaggcatt actatcttca aagacaaacc cactggcgac
    11761 gccatctgca aggttctcaa gagaacagtc agaaaccgta tcagcaagcc caccggtgcg
    11821 ccgcactaac ggcagcgtac cgtacttcaa tccataaagt tgcgttaagc cgcacggttc
    11881 aaaacggctg ggcaccagaa tgacgtccgc gccgcccata atgcgatgcg aaaatgcttc
    11941 gtgatagcca atctgaacgc ccacctggcc ggggtattcc gctgccgccg caaggaaacc
    12001 ttcctgcagc accggatcgc ccgcgccgag tagcgccagc tgcccgccct gctccagaag
    12061 gcccggtaag gcttccagca ccaggtcgag acctttctgg ctggtcagac ggctcaccac
    12121 tgcaaaaagc ggcactttat catcaacctt aagccccatt gcgatttgta actggcgctt
    12181 attttccgct ttatcttcca acgtatcgcg ggtgtagcgc gaggccaaca gtaagtccgt
    12241 ctctggactc cagattttct cgtccacgcc gttcagtacg ccggaaagac gcccttcacg
    12301 gtgacgctgt tgcaacagac cttccatacc gtaggcaaac tgcggttcgg tgatctcgcg
    12361 agcgtaggtt ggactaaccg ccgtaatgtg atcggcatag tacagaccgg ccttcaggaa
    12421 agagatttgt ccgttgaatt ccagcccatg aatattaaag aatgaccatg gcaattggat
    12481 gtcattcata tgatgtgcat aaaacatgcc ttgataggcc aggttgtgca cagtaaacac
    12541 cgacttcgcc ggacgcccgc gcgccgccag atacgcaggc gcaaggcctg catgccagtc
    12601 gtgcgcatgc accacatcag gacgccagaa tgggtcaagc ccgctggcca tttctgcccc
    12661 aacccacccc agcagcgcaa aacgcaatac gttgtcggta taggcaaata agttggtatc
    12721 gtgatacggg cttcccggac gatcatagag atgcggcgcg tcaatcaggt atatgccaac
    12781 cccgttgtaa tgaccgaaca acagcgtgat atgtccggcg aaggtatcac gacgggatac
    12841 tacctgcgca tcggtaacgc cacggcgaat atcgggaaat gcaggcaaca gtacgcgagc
    12901 gtcaacgccg tctgcgattt gtgctgcggg taatgcccca ataacatcag ccagaccgcc
    12961 ggttttaagc agcgggaaca tctctgaaca tacatgtaaa acctgcatta tcgctcctgt
    13021 ttatgcccta acttccgtag catttcgcgc gttaccagca cgatgccttc ttctgaacga
    13081 tagaaacgac gtgcatcttc ctctgcgttt tcaccaatca ccatgccttc cggaataaca
    13141 caagcacgat cgatgacgca gcggcgcaga cggcacgagc gacctaccca tacttccggt
    13201 aacaatacgg cggaatcaat gttgcagaat gaattcacgc gaacgcgcga gaacagaacg
    13261 gactgcacca ccaccgaacc ggagatcaca cagccgccgg ataccagtga gttaagggtc
    13321 atcccgtggc taccggagcg atcctgcacg aatttcgctg gcggtaatga ttcattgtag
    13381 gtgcgaattg gccaattgcg atcgtacata tccagttccg gcaccacaga ggccagatcg
    13441 aggttcgctt tccagtaagc ttccagcgta cccacatcgc gccagtacgg ctcggcatcc
    13501 gggtcggatt gtacgcaaga gagcgggaac gggtgcgcat aggccagacc ggcttcggtg
    13561 atcttgggaa tcaaatcttt gccaaagtcg tggctggagt tctcatcacg atcgtcttct
    13621 tccagcagtt catacagata gtcggcgtca aagacgtaga tacccatact cgccagagat
    13681 ttgctcggat cgttcggcat tgacggtggg ttagcaggtt tttcaacgaa ttcgataatt
    13741 ttatcgttct catcaaccgc cataacgcca aatgcggagg cttcttcaat cggtactggc
    13801 atacaagcaa cggtgcaacg tgcgcctttt tcgacgtgat cgataagcat acgcgagtag
    13861 tcttgcttgt agatatggtc gcccgccagg atcaccacgt attccgcttt ataacggcgg
    13921 ataatgtcga ggttttgggt gaccgcatct gcggtgccgc gataccagtt ttcccctttc
    13981 attctctgct gtgctggcag cagatcgaca aactcgttca tttcttcatt gaagaatgac
    14041 cagccgcgct gaatgtgctg caccagagtg tgggactggt actgggtgat cacgcccata
    14101 cgacggatcc cggagttgat gcagttagac agcgcaaagt cgataatgcg gaacttaccg
    14161 ccgaagtgta cggccggttt tgctcgctta ttggttaaat ccttcaggcg ggtaccacgt
    14221 cctcccgcca gtatcagggc aacagatttc aatggcagct ggcgcgccaa cattaagtga
    14281 tcgttcttct ctaaactaac catgactaac tcctttttta tcatctctgg aacacacaca
    14341 atccgtgtgc aggtccctgc cagacagccg taatcactgg gttatcctct ccagcgaatg
    14401 ggggaatggc gtgccactcc ccagcaggta aaacaatctc tgttacctca agcgtggcgt
    14461 taattgcgat caaaaagcga tccgagagca gaatttgcag ctgtttcggc ccgttttgcc
    14521 actcatccgt gcttaaaggt tgagcatatc gatttagcca acggacattg ccgtcgcctt
    14581 cttcccacca gcgattctcc accaaagcgg gaatgcgctt gcgcagatgg attaacgcgg
    14641 cggtaaatgc ggttaaacca ctgcttgcct gcgaccagtc caaccaggtt aattggttat
    14701 cctgacagta ggcattgtta ttgccatgct ggctgtgacc atgttcgtca ccggccagta
    14761 acatcggcgt accctgggag agcaacaacg ttgttaacag ggcgtgaatg ctgtcgcgcc
    14821 gccgttcaac caggtcaaga gaaccgccta acccttcttt accatgattg ttactgtaat
    14881 tgttgttggt cccgtcgcga ttttcttctc cgtttgcttc attgtgttta tggttgaagc
    14941 aaacgcagtc gcgaagcgta aaaccgtcat gcgcggtgac gagattaatc gcggcactcg
    15001 gcagacgacc attacgttta aaaacatcgc tggaggcagc aaaacgcccg gcaaacgccc
    15061 ccagaggcaa atcataatgt agccagaaac gacgggcagc atcgcggaaa tgatcgttcc
    15121 actcggcaaa cagcggcggg aaatttccca cctgataacc accaggagcg atatcccacg
    15181 gttcagcaat taacttcacc tgcgagagca ccgggcagtt ctggatagcg gtaaacaacg
    15241 gcgcatcctg acggaactct ggcgtacggc ccatgactgc cgccagatca aagcggaaac
    15301 catcgacgtg gcaggtttct acccaataac gcaggcaggc gctggcataa tccaccaccg
    15361 ccggatgact caaattgagc gtgttgccgc aaccggtcca gttgtgataa tcgccgtctt
    15421 ctcttatcca ataatagcta cggttatcga tcccacgcag cgagaataac gggccgtcga
    15481 ggtccagttc cgcactatgg ttgagcacga tatcaagaat gacttcgata cccgctttat
    15541 gcagtgcttt gattgcatcg cgaaactcat ccagcgccgt ttctggcgag caggcatacg
    15601 ccggatgcag cgcaaacatc gccaccgggt tgtaacccca gtagttactt agccccatgc
    15661 gttgcagacg tggttcactg gcaaactgcg ccactggcag cagttccagc gcggtaatgc
    15721 ccaattgttt caaatagttg atcatcaccg gatgcccgag ggctttataa gtgccacgga
    15781 tctcgaccgg gatctccggg tgcaagtacg ttaatccttt gacatgggct tcataaatga
    15841 tggtgctgcc ccacggcgtg cgcggcgggg catcatcttc ccagtcatag tgatcaacca
    15901 ccactacgca tttcggcgca atggcggcgt tgtcgcgata gtcaggttca ttatgaccgg
    15961 cgtgcagcag cgggttatct ttaaactccc cgtcaatttg ccgcgcgcaa ggatcaatca
    16021 acaacttcgc cgggttaaag cgatgcccct cggcgggttg ccaggggcca tgaacgcgat
    16081 aaccataacg caaacccggg cgcgcatccg gcagataacc gtgccaaatg tcgccactgt
    16141 gccctggcaa gtcatagcga tgttcctggc cattggcgtc aaagacacac agttctaccc
    16201 gctcggcatg agcggagaaa agtgtgaagt tgacgccctg accgtcgtaa tgcgcgccga
    16261 ggggagcggg tttgccaatg gcgagttgtg tcattctgcc tcccgaacca gccagatagt
    16321 ggccagcggt ggtagcgtca ggcttagtga atgctgacga ccgtggctgg caatctcatc
    16381 gctgtgtacc gtgccgccat tgcctgcatt actgccgtga tagtgcatgg aatcggtatt
    16441 gaggatttca cgccatttgc ccggctggtt tatgccgaag cgataatcat gacgcggtac
    16501 cggcgtaaag ttactggcaa cgatgatttc gttaccctct ttatcgcgac gcacaaagat
    16561 cagcaccgag cgttctttgt catccaccac cagccattca aagccgtacg ggtcaaaatc
    16621 cagttcatgc attgctttat ggtggcggta ggtgaggttc agatcgcgca ccagacgctg
    16681 gacaccgtgg tgccagttat cgccgccttc caacagatgc cagtcgaggc tggcgtcatg
    16741 gttccactcg cggccctggg caaattcgtt acccatgaac agtagtttct tgcccgggaa
    16801 tgcccacatc cagccatagt aggcgcgcag gttcgcgaat ttctgccatg cgtcgcccgg
    16861 catgcggtcg agaatcgatt ttttaccgtg gaccacttca tcatgcgaca acggcaggac
    16921 gaagttttca gtgtagttgt agagaatccc gaaggtcagt ttatcgtgat gatactgacg
    16981 ataaaccggg tcgagcttca tgtagtccag ggtgtcatgc atccagccga ggttccactt
    17041 gtaccagaag cccagaccgc ccatatcctg cggacgagaa acgccaggga aatcggtaga
    17101 ctcctcagcc attgtcaccg caccggaaac ctgctcacca agaatacggt tggtattacg
    17161 caagaattca atcgcttcaa gattctcgcg cccgccaaat tcgttcggga tccactcccc
    17221 ctctttacgg ctgtagtcgc gataaatcat tgacgccacc gcatcgacgc gcagcgcatc
    17281 aataccaaaa cgttcaatcc agtaaagcgc gttaccgacg aggaagttac tgacttcacg
    17341 gcgaccatag ttgtagatca gcgtgttcca gtcctgatga tagccttcac gcggatcgct
    17401 gtgttcatac aagttcgtgc catcaaattc ggcaagcgca aagtcatcag tcgggaagtg
    17461 gcctggcacc cagtcgagaa tcacgttcag accagctgcg tgtgcggcat caatgaaata
    17521 acggaagtcg tcgcgagtac caaaacggcg ggttggtgca tacaggccgg ttggctgata
    17581 accccaactg ccatcgaagg gatgctcgtt aatgggcagt agttcgaggt gggtaaagcc
    17641 catccattta gcataaggca ccagttgatc ggccagctcg cggtagctca accagaaatt
    17701 gttgtcggtg tggcgacgcc aggaacccag gtgaacttca taaatagaga ttggcgcatc
    17761 aaactgattc gcttttttgc gctcttcagt ctgtacaacc ttttccggca gcccgcaaat
    17821 aagagacgcg gtttccgggc gcatttgcgc ttcgaaggca taagggtcgg acttcagacg
    17881 caagttgcca ttggcatcaa tcatctcgta tttatagagc tgaccgttat gcgccccagg
    17941 gataaacagt tcccagatgc cgctctcttt acgcaggcgc atcgggtgac ggcgaccgtc
    18001 ccagtagttg aattgcccaa ccaccgagac ccgacgggcg tttggagccc agacagagaa
    18061 acgcgtacct gtgacgccat ccatagtatc tgcatgcgcg cctaaggttt catacgggcg
    18121 caggtgagta ccttcagata atagccaggc atccatttcc tggattagcg gaccaaaacg
    18181 gtaaggatca tcaatcaggt tttgctgacc atgccagaca acagccaact gatagcggaa
    18241 aaaattctta cgtcgcggaa tgacgccgct aaagaatccc cgtgagtcga gacactccag
    18301 ttttgcgagt ttgcgcccgg ttttcggttc aatcacccac acatcggtag cgtcgggtaa
    18361 aagggcacgg acttccagtc ccgcggtggt tttatgcatt cccagtacgg aaaaaggatc
    18421 cgcaaaatgg cctgcaatta gcgcgttaat cacgtctcta tcgatacgat cggacatgct
    18481 tgtcttcctg ttttattgtg tcaccccatc caattggatt tttgacttct ggttgtgaca
    18541 tttttttgac ctgaacggcg cagcactctg tgcatcctct ctgcgtcgtc ctcacttcag
    18601 gtaaggctgt gaatactcat gtattcagcc acccttaaag aatagccaat gctctattta
    18661 actcccggta aatcatgaaa catctgcgct tactcctgta ttacgcacta acaggggcgg
    18721 catcgcgccc cagatttaat gaataaagat tacgccagtt gacgaagcat ccgacgcagc
    18781 ggctccgcgg ccccccacag cagctggtcg cccacggtaa aggctgacag gaactctggt
    18841 cccatattca gcttacgcag gcggcctacc ggcgtggtca gcgtgccggt aacggcagct
    18901 ggggttagct cacgcatagt gatttcccga tcgttcggaa cgactttcgc ccacggattg
    18961 tgcgcagcca gcagttcttc cacggtcgga atagacacat cttttttcaa tttaatagtg
    19021 aatgcctggc tgtggcagcg caatgccccg acacgcacac ataaaccatc taccggaatt
    19081 acggaagatg tgttgaggat cttgttggtt tccgcctgcc ctttccactc ttcgcggctc
    19141 tgaccgttat cgagctgttt gtcgatccac ggaatcaggc tacccgccag cggcacgcca
    19201 aagttatcca ccggcagctc accgctacgg gttaaggttg tgactttgcg ttcgatatcg
    19261 agaatagcag aggacggggt cgcgagttca tctgccacat ggccatacag atggcccatc
    19321 tgggttaata actcacgcat atgtcgcgca ccaccgccgg aagcggcctg gtaggttgca
    19381 acggacaccc aatcaacaag atcattggcg aataaaccac ccaacgacat caacatcagg
    19441 cttacggtac agttaccgcc aacaaaagtc ctgatgccat tatttaatcc gtcggtaatg
    19501 acgtcctgat tgacggggtc aagaatgatg atggcgtcat ctttcatgcg cagagacgat
    19561 gctgcgtcaa tccagtaacc ttgccatccg ctttcacgaa gctttggata gatttcgttg
    19621 gtataatcgc cgccctgaca ggtcacaatg atatcgaggg cctttagcgc ctccagatca
    19681 aaggcatcct gaagtgtgcc agtggttccg ccaaaagacg gcgcagcctg gccaagctga
    19741 gaagtagaaa agaagacagg gcgaatggcg tcgaagtcgc gctcttcaac catgcgttgc
    19801 atgagaacgg agccgaccat accgcgccag ccgataaaac caacattttt cataagcgtt
    19861 ttttcctgca aagatgtgtg ctgtataaat gtgccggtct cctcttggca catctttcac
    19921 catacaaaaa gcagccaaag tcgcaagtga aattaatcaa tgatagcgaa gccatcagta
    19981 atgcgactta tcctgctttg ttagcacgca gaaagtccgc ggcaattatc agggaatttg
    20041 agttatgaat gaaatcattt ctgcagcagt tttattgatc ctgattatgg atccgctcgg
    20101 aaacctacct attttcatgt ccgtactgaa acatactgaa ccgaaaagac ggcgggcaat
    20161 catggtgcga gagttgctta ttgctctcct ggtgatgctg gtgttcctgt ttgcgggtga
    20221 gaaaattctg gcatttctca gcctgcgagc agaaaccgtc tccatttctg gcggcatcat
    20281 tctgtttcta atcgccatta agatgatttt ccccagcgct tcaggaaata gcagcgggct
    20341 tccggcaggt gaagagccat ttatcgtgcc gttggctatt ccgttagtcg ccgggccgac
    20401 tattctcgcc acgctgatgt tgctgtctca ccagtaccca aatcagatgg gacatctggt
    20461 gattgctctg ctgctggcct ggggcggcac ctttgtcatc ctgctacagt cttcgctatt
    20521 tttacgtctg ctgggcgaga aaggggtgaa cgcacttgaa cgcctgatgg gattgattct
    20581 ggtgatgatg gcaacccaga tgttcctcga cggcattcga atgtggatga aggggtaacg
    20641 aggagagttt ttcgaaaaga acccggtagc gtcacaacta ccgggcgaac aaacttaact
    20701 caacagctgg aacgcaatca tcccaacgat ggcaccgaca gtgccgagga tggtttccat
    20761 catggtccag gttttcagcg tttcggcttc ggtcgcgccg gtaaatttac cgaacaacca
    20821 gaaaccggcg tcgttaacgt ggctgacaac aatcgaacca ccagcgatac aaatcgacag
    20881 cgccgccatt tgcgcaccgg agtagttcag ttgttcaata accggcatca ccagtcctac
    20941 cgccgttaaa caggctacgg tggcagaacc ctgaatgatg cgcactgcag cggccagcac
    21001 gaagcaggtg atggctatcg gcaggcccat gccagttaac gcttcgccca gtgccggacc
    21061 tacgccagag tcaaccagca cctgtttgaa cacgccacct gcaccaatca ccagcagaat
    21121 gatccccgcc ggttgcagcg cgtgaccgca aatctccatc actttgtctt ttggcatgcc
    21181 ctgacgcatt gccaggccat aaatcgctac cagacaagca accagaatcg cggtaaacgg
    21241 atgaccaata aactcgaacc attcgtaagc ggtagagcct tctggcacaa aacgcgcggc
    21301 aatggttttc agccccacca gcaccagcgg caacaggatc agcgacaggc tgaatccgaa
    21361 agatggcatt ttgccttcgc cgagatgcgg ttcgctgatg tcgtcaggaa tatgcaactc
    21421 cacgtagcgg ctgatgaaat taccccacag cggcccggca ataatcattc ccggaattgc
    21481 cgcacacagg ccaatcagga tcatccagcc aaaatcggcg ttcatctgcg atgccagcag
    21541 cattggcgct ggccctggca ccaggaacgc cgccgctgcc gccacgcctg cgaataatgg
    21601 gattaccagc ttcaccaggt tcgtaccggt gtggcgcgcc attgagaaag caacgctaat
    21661 cagcagaaca atcgccactt caaagaacag cggtagcgca cagaccagcc ccgcaaggcc
    21721 gatggcataa tgcgcgcggc tgtgaccgaa ggatttaagc attttgacgg caatctgatc
    21781 gactgcgccg gtttcatgta agatcttgcc aaacatagct cccagggcga caaccaccgc
    21841 caggaagccg agggtgcctc ccatcccttt ttccatcgtc gctgcgattt tatcgagcgg
    21901 cataccggaa aaaaggccag cccccatgga caccaccatc aaagccagga aagcgtgcat
    21961 acgcgccttc atgactaaaa acagcagcag taaaacagac cctactgctg ttaaaacaag
    22021 cgttaatgta gtcactactt atttgccttt tttaataacc tcaatggtgc ttgccacaac
    22081 accttccagc ggttgatcga tatccaccac cagtacatca gtttcgtccg cacccggctc
    22141 ctgcagcgtt tcaaactgcg tcaccaacat ttgggtttta aagaaatggc ctttgcgcgc
    22201 tttcaggcgg ctttcaatca cgtcaaaatc gcctttcagg tagatgaaag agagattcgg
    22261 attaccttca cgcagcaagt cgcgatagtg ttttttcaat gcagaacaga cgatcagtga
    22321 cactttatta gtgcgctgca tagcaaacgc ggcgtcgttc agcgcctgca accacggttt
    22381 gcgatcgtcg tcattcagcg gttcgccaga cgccattttt tcgatattgc gccgtggatg
    22441 gaggaaatcg ccatcaagaa acgcggcatg aagttgatgc gccacttcac tggcgaccgc
    22501 agatttgccg ctgcccgata cgcccatcaa gacgtaaatg tggtgatcat ggttagtcgt
    22561 gctcaaagtg gtgcccccac aatacaagaa ttaaaaatgt tacgggtaac tgttatcggt
    22621 aacattgtcc agccggacaa tatcagaagc aatatccatc cgtgccttaa gtgtataagt
    22681 gtgagctact tcaaatttgt aggcttaaat agatccgccc ggtgacaagg tgaaacctaa
    22741 atctaacatt ttcggtgtca cagattcgcc acgaatacgc gccagcaggc gttcagcgcc
    22801 aatactgccc atccgctcac gcggcgtcag cacgctcgct aaccgcggct ccatcacctg
    22861 gccaatgtca tgaccgtgga aaccggcaat cgccatatcg tcaggaacct tcaatcccag
    22921 acgctgacat tcaaacgccg cgccgaccgc caggtcatcg ttggtacaga acacgccatc
    22981 cagctgcgga tattcccgcc gcgcctggcg aatcagttca ataccggaag agtaagaaga
    23041 agattgctca accatcacgc tatacggcac cagacctgca tccagcatcg cctgttcgta
    23101 tcccttctgt ttgatgatag tacgttcgtc gagacgcgcg ccgagatagg caatgtggca
    23161 atgcccgcga gcaataatgg cggtggtcat ctggcgtgcc gcttcaaagt tatcaaaacc
    23221 gacggcgata tcgaggcacg gcgactggct gtccatgagt tccaccaccg ggatccccgc
    23281 cacttcaatc atctttaagg tgcgcggcgt gtgggtacgt tcggtgagga tcagtccgtc
    23341 gatattccat gagagcatgg attcgagacg ttcttgctcc atttccggtt tataaccgta
    23401 gtgcgccagc atggtctgat aaccgtgcgc gtcggtgacg ctttcgattc cgcgtaatac
    23461 ttccgcgaaa acctggttgg tgagagaagg taacaggacg ccaatcgccc ggctggtggc
    23521 gttagagagg atatcgggcg cacgattggg aatatagccc agttcatcaa gagcggcggc
    23581 aatcttgccg cgtagagcga cggaaacctg ctccgggttg cgtaaaaaac ggctgaccgt
    23641 cattttggtc acgcctacac ggtcagccac atcctgaagt acgggtcttt tctttttcat
    23701 cgtcctgaag gtacaaaaga gatagattct cttagtttaa cacggacgca tcagaacctt
    23761 cccggggaaa acagtgcttg tttacaatta ttagatatac actaaatcaa ttgcatcgca
    23821 ttgtgctaac gacgcggcaa aaccccggaa gcgtgcatca gcacgcgacc ggagcgagcg
    23881 aggaagcctg caaaaaggca ggcttcaaaa atgccgtgga gctttaaacc ggcggcagat
    23941 cgaacagtaa cacttcgctg tcgctatccg catggatgga gattgcctgc tcatcccaga
    24001 ttgccagacc atcgctggtc gaggctttca cgccgttaat ggtgacattg cctttcacca
    24061 cctggatcca gacgcggcgt tcagcggcaa tctgatgcac cgactgctca tctttcagca
    24121 acgcccagcg gtacagttcc atatcctgat gcactttcaa tgaaccatct cgcgcatccg
    24181 gcgagagcac cagctgtttg ccctgtacgg catcgaagcg acgctgttca taacgcggcg
    24241 taataccgtt ttcttcaggc atgatccaga tctgatacag atgcagacgc tcggtgctgc
    24301 ttgggttgta ctctgagtga cgaatacccg tacccgcact cataatctgg aactcacccg
    24361 ccggaacctg ctctttattg cccatgctgt cctgatgctc aacagtacct tccagcacgt
    24421 aggtcaaaat ttccatatct ttatgcgggt gagtgccgaa gccctgccct gcttcaatca
    24481 cgtcgtcgtt aatcacgcgc agcgcggaga agcccataaa gttcggatcg taatagttgg
    24541 caaaagagaa agtatgccag gagtccagcc agccatgatt tgcatgacca cgttcatttg
    24601 ctttgcgtaa gtagatcatt tgtattcgcc ccctgaatga tttcgatggg cttagtgtgg
    24661 acccgatctg cctgggatga tagagggtga aaattgaccc ctctgttcaa aaaaattgaa
    24721 ctattcgagg ggccatcaaa cttaattcgc gagagtcact gtggagggag aggcttgctc
    24781 aaatccgcgt tcaaggattt ccagattggt aagaacttca gattccttga cgtaatttgg
    24841 cgcaccgtgg gtgatggttt gatacaacgc atcataaacg cgcccgtaat cgcccatctc
    24901 cggcttcatc tcttctctga ccgtcacgcc ctcgtcattg acatactcca gcacaccgac
    24961 cgaattatcc gctgcgaatc ccggttcgcc cggcataata ttagccttca ggctggtttc
    25021 ctgctggtcg ataccgtatt taataaacga acctttctta ccgtgaacga taaatttcgg
    25081 ataatcgatt ttcaccagat ggctggtttt gacgatggct ttcaggtcgc cataaaacag
    25141 ttgcgcttca aaggtgtcgt caggattggc tttattacgc aggctgcgga tgtcataagc
    25201 gacgtgatcc gggcgaccga acagagaaat aatctggtcc atcgtatgca caccaaggcc
    25261 ataaaacgcg ccatcctgcg gcagcccagg tttggtttct gccaccgggc ggtaatagtc
    25321 aaaatggctt tccacttcaa caatctctcc caacttgcca ctttcaatcg cttttttcgc
    25381 tgtcaggaag caggagtcaa agcgacgatt ctgatacggc gtgacggtca gccctttgct
    25441 tttcgccaac gcaaacagct ctttcgcctg cgcaagtgtc ggagtgaacg gtttttcgac
    25501 cagcacattt ttcccggctt ccagcgcgcg cttcgcgtac tcgaagtggc tgtccgcgtg
    25561 ggtgcagaca acaaccagct taacatcggg atcgtttagt acttcgtcga gatcgctggt
    25621 gaaatggata tgggaataaa tgggagcctg ttcttccggc ttcgcatggc gacgaaaaat
    25681 atgcgcgaca tgccagctat ccttgcggtt aagtacatac ggcagatggt aacgggtggt
    25741 gcttttgccg aagccaataa aggcgcagtt gatgaccatg atttcgtcct ttttaaggtg
    25801 gttatttaca ccttagcgca aagcggacgt ggttcctact ggagtgcgca taacgaacac
    25861 aagcactccc gtggataaat tgagaacgaa agatcaaaaa aaaagccagc acccggctgg
    25921 ctaagtaata ctggaagcaa tgtgagcaat gtcgtgcttt caggttctcc gcgagggtct
    25981 tcctgatcgc gagacaataa taatcattct cattcgcact tgtccaacac tttttgcaaa
    26041 aaaatgcatt tgactcgcat ttgaaagtca atgatgttga aagggacatt taccccaaag
    26101 aggacaaagg aatgagtgag atagtaatac gccacgcaga aacacgggat tacgaggcca
    26161 tcaggcagat tcacgcccag ccggaggtgt attgcaacac actacaggtg cctcatcctt
    26221 ccgatcatat gtggcaggag cgactcgccg atcgtcccgg catcaagcaa ctcgtcgcct
    26281 gtattgatgg agacgtcgtg ggccatctca ccattgacgt gcaacagcgc ccacgccgca
    26341 gtcatgttgc cgattttggt atctgtgtcg actctcgttg gaagaaccgc ggcgtcgcca
    26401 gcgccctgat gcgagagatg attgaaatgt gcgacaactg gttgcgggta gatcgcattg
    26461 aactaaccgt gtttgtcgat aacgccccgg caattaaggt ctataaaaaa tacggctttg
    26521 aaattgaagg gactggtaag aagtacgcat tgcgtaatgg tgaatatgtc gatgcatatt
    26581 atatggcgcg ggtgaagtaa gatagtgccc tttttctgag atggaaaaag ggtgtcattc
    26641 aaaatcgaca taccttcctt taaggtattt atttgcccaa tacatatatt gagat
//
LOCUS       DAZGOB010000062        26659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGOB010000062 DAZGOB010000000
VERSION     DAZGOB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.062"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(17..799)
                     /gene="yjjV"
                     /locus_tag="ACHLIF_002722"
     CDS             complement(17..799)
                     /gene="yjjV"
                     /locus_tag="ACHLIF_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9606715.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(796..1869)
                     /gene="yjjU"
                     /locus_tag="ACHLIF_002723"
     CDS             complement(796..1869)
                     /gene="yjjU"
                     /locus_tag="ACHLIF_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO9606716.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(1991..2152)
                     /gene="ytjA"
                     /locus_tag="ACHLIF_002724"
     CDS             complement(1991..2152)
                     /gene="ytjA"
                     /locus_tag="ACHLIF_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO9606717.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(2279..2884)
                     /gene="osmY"
                     /locus_tag="ACHLIF_002725"
     CDS             complement(2279..2884)
                     /gene="osmY"
                     /locus_tag="ACHLIF_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO9606718.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(3277..4866)
                     /gene="prfC"
                     /locus_tag="ACHLIF_002726"
     CDS             complement(3277..4866)
                     /gene="prfC"
                     /locus_tag="ACHLIF_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO9606719.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(4957..5634)
                     /gene="yjjG"
                     /locus_tag="ACHLIF_002727"
     CDS             complement(4957..5634)
                     /gene="yjjG"
                     /locus_tag="ACHLIF_002727"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO9606720.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(5649..6095)
                     /gene="rimI"
                     /locus_tag="ACHLIF_002728"
     CDS             complement(5649..6095)
                     /gene="rimI"
                     /locus_tag="ACHLIF_002728"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO9606721.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(6064..6477)
                     /gene="holD"
                     /locus_tag="ACHLIF_002729"
     CDS             complement(6064..6477)
                     /gene="holD"
                     /locus_tag="ACHLIF_002729"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO9606722.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            6580..7611
                     /gene="rsmC"
                     /locus_tag="ACHLIF_002730"
     CDS             6580..7611
                     /gene="rsmC"
                     /locus_tag="ACHLIF_002730"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO9606723.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVAKADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            7912..7998
                     /locus_tag="ACHLIF_002731"
     tRNA            7912..7998
                     /locus_tag="ACHLIF_002731"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7946..7948,aa:Leu,seq:cag)
     gene            complement(8037..8273)
                     /gene="yjjZ"
                     /locus_tag="ACHLIF_002732"
     CDS             complement(8037..8273)
                     /gene="yjjZ"
                     /locus_tag="ACHLIF_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO9606724.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMLMNLNQG"
     gene            8414..9202
                     /gene="fhuF"
                     /locus_tag="ACHLIF_002733"
     CDS             8414..9202
                     /gene="fhuF"
                     /locus_tag="ACHLIF_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO9606725.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(9240..9917)
                     /gene="bglJ"
                     /locus_tag="ACHLIF_002734"
     CDS             complement(9240..9917)
                     /gene="bglJ"
                     /locus_tag="ACHLIF_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO9606726.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMTRISFAAVIFSFSAMRSERREGLSCLTELAIKLPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(9875..10600)
                     /gene="yjjQ"
                     /locus_tag="ACHLIF_002735"
     CDS             complement(9875..10600)
                     /gene="yjjQ"
                     /locus_tag="ACHLIF_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9606727.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            11219..11989
                     /gene="yjjP"
                     /locus_tag="ACHLIF_002736"
     CDS             11219..11989
                     /gene="yjjP"
                     /locus_tag="ACHLIF_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO9606728.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            11980..12453
                     /gene="yjjB"
                     /locus_tag="ACHLIF_002737"
     CDS             11980..12453
                     /gene="yjjB"
                     /locus_tag="ACHLIF_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO9606729.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            12560..13099
                     /gene="dnaT"
                     /locus_tag="ACHLIF_002738"
     CDS             12560..13099
                     /gene="dnaT"
                     /locus_tag="ACHLIF_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO9606730.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            13102..13839
                     /gene="dnaC"
                     /locus_tag="ACHLIF_002739"
     CDS             13102..13839
                     /gene="dnaC"
                     /locus_tag="ACHLIF_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO9606731.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            13888..14382
                     /gene="yjjA"
                     /locus_tag="ACHLIF_002740"
     CDS             13888..14382
                     /gene="yjjA"
                     /locus_tag="ACHLIF_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO9606732.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            14636..16927
                     /gene="opgB"
                     /locus_tag="ACHLIF_002741"
     CDS             14636..16927
                     /gene="opgB"
                     /locus_tag="ACHLIF_002741"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO9606733.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGAGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(17066..18088)
                     /gene="lgoD"
                     /locus_tag="ACHLIF_002742"
     CDS             complement(17066..18088)
                     /gene="lgoD"
                     /locus_tag="ACHLIF_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO9606734.1"
                     /translation="MSTMKVLICQEIKNLVWTVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNFHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            18227..19132
                     /locus_tag="ACHLIF_002743"
     CDS             18227..19132
                     /locus_tag="ACHLIF_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO9606735.1"
                     /translation="MSRSQNLRHNVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(19303..20955)
                     /locus_tag="ACHLIF_002744"
     CDS             complement(19303..20955)
                     /locus_tag="ACHLIF_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO9606736.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRTSTENLDNELIMETGARVIGYMRRSEDDMSENDNSLHMQRAKLERTFNL
                     AALNARRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
     gene            21635..22606
                     /locus_tag="ACHLIF_002745"
     CDS             21635..22606
                     /locus_tag="ACHLIF_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO9606737.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            22670..23143
                     /locus_tag="ACHLIF_002746"
     CDS             22670..23143
                     /locus_tag="ACHLIF_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO9606738.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVMTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVAVLSIALLLYMAWLG
                     YRLAEMAQFKVTQLLKVSWFWIDLAVPVGALATAIYTLYWLIHDIKQSDSGDKMQ"
     gene            23140..24411
                     /locus_tag="ACHLIF_002747"
     CDS             23140..24411
                     /locus_tag="ACHLIF_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO9606739.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLTLVTYIPAISLFLV"
     gene            complement(24454..26118)
                     /gene="tsr"
                     /locus_tag="ACHLIF_002748"
     CDS             complement(24454..26118)
                     /gene="tsr"
                     /locus_tag="ACHLIF_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO9606740.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSGENWETF"
     gene            complement(26237..>26659)
                     /gene="hpaR"
                     /locus_tag="ACHLIF_002749"
     CDS             complement(26237..>26659)
                     /gene="hpaR"
                     /locus_tag="ACHLIF_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO9606741.1"
                     /translation="LLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESPSMDFHDLA
                     YRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRAQTQIEEAY
                     RQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         6664 a         6913 c         6420 g         6662 t
ORIGIN      
        1 gcctacgaag ggtaaactac ccactaacgc taaacaacgc atacgtatta ttaagcaaca
       61 cttcggcaat ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgca
      121 cagcctgttc cggtcggtta ggctgcccct gaaagccgtt gagcggcata tccggcgcgt
      181 cggtttccag caacaacgat gccagcggta attttgcgat gacatcgcgg gttttactgg
      241 cgcgtggata ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct
      301 cggcctgttg caggctgccg gaaaaaccgt gaaccacgcc agtgcacggt aaatcgtgac
      361 gtttaagatg catcgccagt ttgtcgtgtg tacgccggga atgaaggatc accggcagat
      421 cgtagcgttt cgccagtttc agctgttcgt cgagtaacca ctgctgcctc tcaaattgcg
      481 gatcgtcgcc gaagagatcc agaccgatct cccccaccgc caccaccttc gccggacgcc
      541 tttccagcgc ctgctgtagc tgatcaagag acacatcgct atgtttttcc aacatccccg
      601 gatgcaaccc caatgcggca tacagcggtt gatatttttc cgctaacgcc tgcacgcggg
      661 caaaattttc cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc
      721 gttgcaggct ggcctcttca tcgccactaa acggcgggaa atcaaaatgg cagtgggtat
      781 cgataaaacg gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg
      841 agcatcgctc acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaataacgat
      901 cgattccggc gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag
      961 cttgccaacc gtggcgagga aataacgacc gcataaacgc ccaagtttat agtcttcacg
     1021 cagcgccgga atccgactgc caagcgcgat actatgtaat ggtttcggtg gataaatttc
     1081 gaatatccgc agcttgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt
     1141 ttcatgatgc tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc
     1201 catgcgtttg aaccactgcg gcgtgtagta catttgtgac ggcacagtgc ggatgacgac
     1261 caacgtttta gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc
     1321 atccaggtag ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact
     1381 ggaggcgcga atcacatcca gccagttttg tttggttggt aaaaagtaat taggcgcgta
     1441 gtcatcctga cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc
     1501 ggtatccatt tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat
     1561 aagatttcct ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg
     1621 catgatgact ttgcgcgcgt aaccgggctg attgcagata aacgccgaga ggttctgcgc
     1681 cccggcagat gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc
     1741 cagcacgcca gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg
     1801 tccaggctgg aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg
     1861 ctgccccacc tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat
     1921 tattcgctaa aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat
     1981 ggtctgaaat ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc
     2041 cgacgacaaa gacaatttta gctgcgcctg cagcggtacc ggccagacca ccaaacccaa
     2101 gtgcggcggc gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct
     2161 cctttaccat agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt
     2221 tttagtgtgg tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt
     2281 acttagtttt cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt
     2341 cagcacggtc gctctgtgcc tgggaatcga cggtaccgga gagctgaacc acgccgtcgg
     2401 tggtttcaac tttcacatga cgggaaggga cgatatcgtc cgccagcagt ttggctttga
     2461 tttcactggt ggtggcggtg tcacccgcgt agcccttcac agagccttct ttagcgtcgc
     2521 gaacgtgcag tttgtcgctg acagaggtca ccccttcaac gcctttcgcc actttcactg
     2581 cctcttcggc ctgagcctgg ctttcaacga aaccgctcag ggtcacgact ttttgatcgg
     2641 ttttgacaga gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt
     2701 tcgcggtgat ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt
     2761 tttgccctgc gctttcattg gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg
     2821 cgacggcaga ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag
     2881 tcatcgattt attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt
     2941 gctgaatagg gagagacttc accctctaca gaagtcaatg gtcgccatca caaaagcgat
     3001 gggtgatgaa taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca
     3061 atcgacggct ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg
     3121 tcattacttt gttaaatata gatcacaatt ttgaaaccgc tcgggatatc agcgaaaaca
     3181 taagcaaaag tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga
     3241 cagggcgcag cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa
     3301 ctgaacgtcc ggataacgtt cctgcgccag gcgcaggttg accatgctgg tagcgatgta
     3361 agcgaggtta tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc
     3421 gaatttcttc gcgtctgcac attctaccca gcgggcggtg gcaacgttga ctgattcata
     3481 tactgcttca acgttgtatt cgctcttcag gcgcgatacc actacatcaa actgcagcac
     3541 accaactgca ccaacgatca gatcgttgtt ggagatcgga cggaacacct gcaccgcgcc
     3601 ctcttcggaa agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag
     3661 gcggatacga cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc
     3721 accctgggta aaggtgtcgc cgatctgaat ggtgccgtgg ttgtgcaggc cgaggatatc
     3781 gccaggatac gcttcttcaa cgtgcgaacg gtcacccgcc ataaaggtca gcgcgtcaga
     3841 gatcaccacg tctttcgcgg tgcgcacctg gcgcagcttc atgccttttt catatttacc
     3901 ggataccacg cgcataaacg ccacgcggtc gcggtgtttc gggtccatgt tggcctgaat
     3961 tttaaatacg aagccggtaa atttgtcttc gctcgcttct acggtacggg tatcagtctg
     4021 acgcggcatc ggcgcaggag cccactccac caggccatcc agcatatgat cgacaccgaa
     4081 gttacccagc gcagtaccga agaataccgg agtgatttcg cccgcaagga acaattcttt
     4141 gtcgaactcg ttagacgcgc ctttcaccag ttccagttcg tcacgcagct gctgcgccag
     4201 atcttcacca accgcagcat cgagatccgg gttattcagc cctttaacaa tgcggacttc
     4261 ctgaatggtg tgacctttac cgctctgata gagataggtt tcgtctttat aaaggtggta
     4321 aacgccttta aacagcttgc cgcagccaat cggccaggtg atcggtgcgc agccgatttt
     4381 cagctcgttc tcaacttcat cgagcagttc catcgggtcg cggatatcac ggtcaagttt
     4441 gttcataaag gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt
     4501 acgatcttca acaccttttg cggcgtcgat aaccatcagg cagcagtcca ccgccgtcag
     4561 ggtacgatag gtatcttccg agaagtcttc gtgccccggg gtgtctagca ggttaaccag
     4621 gcaatcgtga tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc
     4681 catctccatc cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccggc
     4741 ggtctgaatg gcctgtccga acagcagcac cttctcggtg atggtagtct taccggcgtc
     4801 cgggtgagaa ataatggcaa aagtgcggcg cttagccacc tcttgcaaat aaggagacaa
     4861 cgtcataatt ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa
     4921 ttgcggctat tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc
     4981 cagttcgtgc aacgaagaaa cggtccaggt gggcgcgatg ccttctggtt gctcgcgatt
     5041 gtgcgcattc agccagcagg tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc
     5101 ggcagtgtcg ccaaccatca gcacgcgtga acggtcagga ttgcccgcct gttccagcgc
     5161 ataatcgaaa attttcttat tcggtttagc aacgccaact tcttcggaaa tcaccagcaa
     5221 atcgaagtaa tcacgcaggc ccgtgcgttc cagacgcact tgctgcaagg cgctaaagcc
     5281 gttggtgatg atgccgattt tggcgttgcc acgaatggcg ttaagcagag aaaccgcgcc
     5341 cggcagcggc gtgcagattt ccgccatcgc attaataaag gcatcgttaa gcaagcctgc
     5401 ttcaactttc agccgttcgg cccagctctc gaaacgcccg tgctgaagct gtaatgaagt
     5461 gatcgcgccg ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa
     5521 atcttcagcg gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga
     5581 gtcaaaggta aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc
     5641 accttgtctt acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg
     5701 gtggggtagt aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca
     5761 atggcggcag catttgaggc acggacttcc agccagagcg tcgccacgcc gcgtttttcc
     5821 agttcgtcga tcagatgttc cagcagcgcc cttcccaatc cctgacgctg atagtcagga
     5881 tcgaccgcaa tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc
     5941 gccattttgc cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca
     6001 aaggtttttt cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt
     6061 aaatcagtcg tttcgaggga agaaatcgtg ttcatatgtg caaatttgtt gccataacgc
     6121 ggcgcgtgcc gttgggtttg cccgtaaatc ggtgagcgcc ggtgatgcca cctgagcgcc
     6181 ttccagtgat agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg
     6241 cagcatcgcg attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc
     6301 gcgcagaaca tcgctcacta aaggatcagt cagggcggga agatcgtttg ccaccatcac
     6361 cagacggacg tgtgccggga tcgcaatggc aatttccccc tgcaacgcgc cagggcgacg
     6421 cagcgaccac tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc
     6481 gaatcgctcc tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata
     6541 tggctataat cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca
     6601 agtgaagtct tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga
     6661 gacttacagg atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag
     6721 caattccacc actggcaggt attaagccgc cagatggggg ataacgcccg ttttagtctg
     6781 gtcgccaagg cggatgacgt cgcagattgc gatacgctga tttactactg gccgaagaac
     6841 aaaccggaag cccagttcca gttgatgaat ttactttctc tgctgccggt ggggacggat
     6901 atttttgtcg ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat
     6961 tatgcgccgt tgaataaagt cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg
     7021 gaaaaacagc cagtatttga tgcggataaa ttctggggcg aatacagcgt cgatggcctg
     7081 acggtcaaaa cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg
     7141 ctgctctcga cgttaacccc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg
     7201 ggtgtgcttt cagttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat
     7261 gtctctgccc cggcggtaga agccagccgt gcgacgcttg cggccaacgg tgttgaaggt
     7321 gaagtctttg ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc
     7381 aacccgccgt tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgc
     7441 ggcgcggtgc gtcatcttaa tagcggcggc gagctgcgaa ttgtagcgaa cgccttcctg
     7501 ccttacccgg acgtgctgga tgagacattt ggcttccatg aagttatcgc gcaaaccggg
     7561 cgcttcaagg tgtatcgcgc cattatgacc cgccaggcga agaaaggtta agtatcccgc
     7621 cgcaagcctt gtcggatgcg gcgtgctcgc cttatacaac ctacaaaacc tgcacgttag
     7681 cccttcgtag gccagataag acgcgcccgc gtcgcatctg gcatctgcac ccaaataccg
     7741 tgtcggatgc ggcgtgaacg ccttatccga cctacaaaac ctgcacgtta gtttcctgat
     7801 ggacattttt ccagcaatta cacctccggc gataattaac tattgacgaa aagctgaaaa
     7861 ccactagaat gcgcctccgt ggtagcaatt ctttttaaga attgatggta tgcgaaggtg
     7921 gcggaattgg tagacgcgct agcttcaggt gttagtgtcc ttacggacgt gggggttcaa
     7981 gtcccccccc tcgcaccaat tatctttact tcctttcttg tttcttcctt gatttttcat
     8041 ccctgattca aattcattag catcagcatg atgccagcaa taagcgccag gcacgatggt
     8101 agcaaaacgt aatgccgtaa ctgtttgtga tacagcatca ccggcgtcaa tatcaatccc
     8161 atcagaatga caatccgcca ttgatccggc gataaatccg catacatcag ccctgcaatc
     8221 agcaatcccg gcagcaacac tccccagcca ctgcccagcg tacgttgcaa catggtttca
     8281 tctctttcat tgataatgat aaccaatatc atatgataat ttttatcatt tgcaagccag
     8341 agaaatccct tgctatcggg taaacctatc gctaggatta gcaatcatta tcatttagat
     8401 tactatcccg attatggcct atcgttccgc accgctctat gaagatgtca tctggcgaac
     8461 gcatctccag ccgcaggatg ctgggcttgc acaagccgta cgggcgacga tcgccgaaca
     8521 tcgtgaacat ttgctggagt ttatccgtct ggatgaaccc gccccgcttc gcgccatgac
     8581 gctggcgcaa tggtcatcac cgaatgcgct aagttctcta ctggcggtct attccgatca
     8641 tatctatcgc aaccaaccaa cgatgatccg cgagaacaag ccgctgatct cactatgggc
     8701 gcaatggtat atcggcctga tggtgccacc attaatgctg gcgctgctga cgcaggaaaa
     8761 agcgttagat gtgtcgccgg aacatttcca cgttgagttt cacgaaaccg gccgtgccgc
     8821 ctgtttctgg gtcgatgtgt gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg
     8881 aatggaaacg ttaatcagcc aggcgctggt tccggttgtg caggcactag aagcgacggg
     8941 agagatcaac ggcaaactta tctggagtaa taccggttat ttgatcaact ggtatctgac
     9001 tgagatgaaa cagctgctcg gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt
     9061 tgagaaaacg cttactaccg gtgaagataa tccactctgg cgtaccgtgg tactgcgcga
     9121 cggcctgctg gtgcgccgca cttgctgcca gcgttatcgc ttaccggatg tgcagcaatg
     9181 tggcgattgt acgctgaaat gaaagcactg ccggggaagt aaacccggca tcatgcggat
     9241 taatagggat gcaacacatt acttgcttcg caatgccgca tacataacag aatatctgcg
     9301 gcctccaaca accctgcatc agaactgacg cccagtttcg acatcacatt aaatttatgt
     9361 gcacggatcg ttttgatatt acgtttaagc tgctcggcaa tttgtgtcat tgagtaacca
     9421 cgcgacataa agcgcaatat ttctctctcc gtcgggctta gcgtccggct ttggttaatg
     9481 taccactgat tgttcagtcg gtcggtcgcc tgacgtacac catttaatga caaaaagagt
     9541 tcctgatgaa aaatctccag cgttgacgct ttacttaata caccgtccag cggtgatggc
     9601 gacaatgaac caatcagccg agcttcaata tcatcatccg caataactaa acgccgggta
     9661 cgcggaagct taatcgccag ttcagtcagg caagataatc cctctctgcg ctcacttctc
     9721 atggcagaaa aagaaaaaat gaccgccgca aacgagatcc gcgtcatggc atcctgaaat
     9781 gactcctgac tggtataggt atggagctta taagggttac ccgcaaactt tctgaataaa
     9841 ctctcaatac cgatactact catgacgcat ttttctatga gtgcgacatt tctcttctta
     9901 attcggctgt gttccattct gcggcatctc cataaacaga aggcaagtta agttctcttg
     9961 caccctgcgc gctatttatc catgcataca tttcagcgtt gctgtgaatt gccagtcgac
    10021 gcatcgcact gtttttttgg gcgctgatag ttttattact tttcttaagc aatgaagcta
    10081 tctggttgat gccccatcct ttacctaata gccgcagtac cttgcgctct gaaagagtga
    10141 gaatgacgga gcgatagcta aaatcatgag tttcagtcat tgccggagag gtcagcatgg
    10201 cgctgatacg ctctgcgtgc gtattgccgg aacgtacggt cttgaccaga ttctcaatgg
    10261 gttcaacatc agacaataac gtcgccgtag ggcacatgag cagttctact gcctgggaat
    10321 accaggagcg tgacaccatg aaaacccagt gaatttcccg atattgtgag attaaagaat
    10381 aataatgttc acaaacagaa cgtggatctt cactttcacc ggctaaatca gcaatgacta
    10441 atccggaacg gcgtaattgt aataaggtaa ggtcctccgc agaggcgctg gatattattt
    10501 catattcagg gaagtgagtc ctcatgacct cttttaaccc tgcttgcata acaggtattt
    10561 tactgataac aattccattt ttgcagcatc ctggcaacat attccctcca catccactgt
    10621 ctaaaaatta tgatggttta ttcaggatat tcattcctta aatgtttcca gcaaattaat
    10681 agattaagcg aataaattat ccttagcatt cactttcata tcagcatact cctgctgcac
    10741 aacaactcca ctgcatgtgt cagaaggtta gcatcaaatg ataacctggt gaaatagcct
    10801 ggagacattt aaaatatcta cattaagttg ttaggaatac ttaatgaaaa acagaaatca
    10861 cattcattta atttataatt aaatgatatc ccttcctgca gaatgcaaaa tcacacaacc
    10921 aatatttatt caatgaaatc tgatggtttt aacttgttaa cattaaaaaa cgtgcccggt
    10981 tatctattat cctgattcgt aaaggccaaa caataaattc attatgatga taatattcat
    11041 gtgatagttt gtttgcggcg agagataatt cgctttttat caccgagctt ctcccgataa
    11101 ccaggattaa tcagcgcaat tttgatctta acctaccgca aaacaaccgt gcttcatgat
    11161 acaaatggat tctcattatc tgaacaatac gcagcacgtc tatgacaaag ggcgagttat
    11221 gcaaactgag caacagcgag ccgtaacacg gctttgtatc cagtgtggat tatttctttt
    11281 gcaacatggt gcggaaagcg cgttggttga tgagctttcc tcacgactgg gtcgggcgct
    11341 gggaatggac agcgtcgaaa gttctatctc ttcgaacgcc atagtgctga caaccattaa
    11401 agatgggcaa tgcctgacat cgacacgtaa aaatcacgat cgcggcatta atatgcatgt
    11461 ggtgactgaa gtccagcaca ttgtgattct tgcggagcat catctgctgg attacaaagg
    11521 cgtagagaaa cgatttagcc aaattcagcc attacgttac ccaagatggc tggtagcctt
    11581 aatggttggc ctttcttgcg cctgtttctg taaactcaat aacggtggct gggatggtgc
    11641 cgtcatcacc ttctttgcca gtacgaccgc gatgtatatc cgccagctgc tggcacaacg
    11701 tcatcttcat ccacagatca acttttgcct taccgctttc gccgccacca ccatttccgg
    11761 attgcttttg caactcccca ctttcagcga tacacccacc attgcgatgg ccgccagcgt
    11821 tctgctgctg gtgccgggct ttccgttgat taatgccgtc gccgatatgt ttaaaggcca
    11881 catcaatacc ggactggcac gctgggcgat cgccagtctg ctgacgctgg ctacctgcgt
    11941 cggcgtggtg atggcactga cgatttgggg gctacgcgga tgggtgtgat cgaatttctg
    12001 ttagcgttgg cgcaggatat gatcctcgcc gccattcctg cggtcggctt tgcgatggtg
    12061 ttcaacgttc ccgttcgggc gttacgctgg tgtgcgctgc ttggcgcgat tggtcatggt
    12121 tcacgaatga tcttaatgac cagcgggctg aatattgagt ggtcaacttt tatggcttct
    12181 atgctggtcg gcaccattgg tattcaatgg tcgcgctggt atctggcgca tccgaaagtg
    12241 tttaccgtgg cggccgttat ccctatgttc ccgggcatat cggcttatac cgcaatgatt
    12301 tcggcggtaa aaatcagcca gttaggttac agcgaaccgt tgatgattac cctgttaacc
    12361 aactttctta cagcttcatc gattgttggt gcgttatcca tcggtctttc cattcctgga
    12421 ttatggttgt accgcaagcg ccctcgcgta taaaattgcc tcgcttaagc acacggatga
    12481 gagacagcct cctctcctcc gtgtgttact ataaaagtta tctcccttct cgttcatcgt
    12541 tccatatttg agaaacagta tgtcttccag agttttgacc ccggacgtcg ttggtattga
    12601 cgccctggta cacgatcacc aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt
    12661 atttgctaac aatgccccgg cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct
    12721 ggcgctggaa gaaaagctgg cgcgtccggg aagcgatgtc gctctggacg atcaactcta
    12781 tcaggaaccg caagccgctc ccgttgctgt ccccatgggg aaattcgcca tgtatccgga
    12841 ctggcaaccc gatgccgact ttatccgcct ggcggcgcta tggggcgtgg cgctaagaga
    12901 gccggtgacc accgaagaac tggcctcatt cattgcctac tggcaggcgg aaggtaaagt
    12961 ctttcaccat gtgcagtggc aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag
    13021 caacggcgga ctgccgaaac gagatgtgaa tacggtcagc gaacctgaca gccaaattcc
    13081 accaggattc agagggtaac gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa
    13141 atgatgcctg cccatatcaa acctgcattc aaaacgggtg aagagctact ggcctggcag
    13201 aaagaacaag gggcgatccg ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg
    13261 cagcgcacct ttaaccgctc cggtattcgt ccactgcatc agaactgctc ctttgagaac
    13321 tatcgcgttg agtgtgaagg gcagatgaat gcgttaagca aagcgcgcca gtatgtcgaa
    13381 gagtttgacg gcaacatcgc cagcttcatc ttttctggta agccaggaac cggcaaaaac
    13441 catctggcgg cggcaatctg caacgagctg ctgctacgcg gtaaatccgt attgatcatc
    13501 accgtggccg atattatgtc ggcgatgaaa gataccttca ggaatagcgg taccagcgaa
    13561 gaacaactgc ttaacgatct gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg
    13621 cagaccgaat cgaaatacga aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct
    13681 tccaaacgcc caaccgggat gctgaccaac agtaatatgg aagagatgac caagttgctg
    13741 ggcgaacgcg tgatggaccg tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg
    13801 gatagctacc gtagccgggt aacaggtaaa gagtattaat ccccgtttct ggcggtatac
    13861 tgcgacacaa ttccaccttg agtaatgatg atgaaaactg ttaaacatct tctgtgctgt
    13921 gccattgccg ccagcgcatt aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg
    13981 ctctccagcg ccgccagcga acttggcaac caaaacagca cgacacagga aggcggttgg
    14041 tcgctcgcgt cattaactaa cttgcttagc agcgggaacc aggccttaag cgcagataac
    14101 atgaacaacg ccgcaggcat tctgcaatac tgcgcgaagc aaaagctggc gtcggtaacc
    14161 gatgccgaaa acatcaagaa ccaggtgctg gaaaagctgg gcctgaacag tgaagaacaa
    14221 aaagaagaca ccaactatct ggatggcatt cagggtttgc tgaaaaccaa agatggtcag
    14281 caactcaatc tggataacat cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc
    14341 tgcgatctgg tgttaaagca ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt
    14401 ttagctgtta cccggctgac gcggctctct cctgcccttt tccctgctcc tggacggttt
    14461 tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa ctaattccta
    14521 acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca ttacattgtg
    14581 cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc gcccgttgtc
    14641 agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat ggaaagcggg
    14701 acgtaacacc tggtggtttg cagccacatt aacggtgctg gggctatttg tcgttttaaa
    14761 tatcaccctg tttgccagcg actattttac tggcgctggt attaacgacg cggttctcta
    14821 taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg
    14881 cattgtgctg gggctgacag cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg
    14941 ccatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc tgggttcggt
    15001 ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt cacgcgatgg
    15061 cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg acccgaaact
    15121 caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata acgaagcttt
    15181 cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac
    15241 gcagcagctg ccgggaacag attacacgat tgcgggcatg gtggcttctc agtgcggcat
    15301 tccgcttttt gccccatttg aaggtaacgc ctccgcttct gtttccagct tcttcccgca
    15361 gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg
    15421 cgcgaatctg cgttttgccg gtaaagatgt gttcctgaag tcgcatggct tcgaccactt
    15481 atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca acgactgggg
    15541 attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc
    15601 aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg atggttttat
    15661 ctcgcgtacc tgtaaccgca aaaaatatga ttttgacggt aagccgaacc agtcattcag
    15721 cgcggtaagt tgcagccagg agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc
    15781 gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga acaacacggc
    15841 gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc gtggcgacaa
    15901 gccgcagcaa gagacgctgg cagtgaagcg taacacgatg gataacggcg cgacggtgct
    15961 ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat ccgggcagtc
    16021 aatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc cggatatcat
    16081 ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc agaaaaacat
    16141 gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt cagacaaacg
    16201 cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc tggccgattt
    16261 cgctccacgc gacaatttcg tctgggttga ccgttgctac aagatggcac aactctgggc
    16321 tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg gcggtcagca
    16381 aattgttcag catgttgaca aaacaacatg gcagggcaaa acggcattta aagatacggt
    16441 catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg ataacgatat
    16501 tcgctacaaa gccgacagtt tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca
    16561 gtttagtggg atttcccgtc cagagtcgtg gggccgctgg tccaacgcgc agctgggcga
    16621 tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg tgattaccgc
    16681 caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca atgaagaaca
    16741 aacccttgtg ctgggcaatg aagtgaccac caccacactg catttcgata acccaaccga
    16801 tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag ggaatatcct
    16861 cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagttg tggaacgtga
    16921 agggtaattc ctgactgatg tgtaggtcgc ataaggtgcg acctacacac cttctccggc
    16981 ctacaaaaca tcattgagaa acagaaaaaa acagcgccca acacttcccc ggacgctgca
    17041 tggcaacttt aataatctcg ccttatcaga aagtaattac acctttaatt aactcacgat
    17101 tgttaatcac atcgcgctcg taggtttctg ccagcgtggc gaacggatag cgatgggtta
    17161 acatcatgtc agcagtgatt tttccttccg ccatcagtcg accgacttta gcaaaatctt
    17221 ccggcgtggc gttgcggctg cccatcatcg tcgtttcttt tttatggaat tccggatcgg
    17281 agaactgcaa ctcaccttta aacaggccga caaataccac cgtgccgccg tgacgaatca
    17341 gattcacggt gttattcatc gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca
    17401 gcgaaccacc aaactgcgcc cgcaactgcg cgtcaaaatc ttcggctgac gggtccagca
    17461 cgggcaactc cagacgcgtt gccacatgtt cacggcgcgc cggactggta tccgccacca
    17521 ccacctgtgc gccatcggct ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc
    17581 cgaccaccag cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa
    17641 tagcgaaagg ttcaatcaat gcggccgcct gcgggtcaat accgtctgcg ggcaaaatgt
    17701 tcgccaccgg cacgctcaaa tactcactaa aaccgccatc ctgatgcacg ccaatgactg
    17761 aaattttttc acagcaattg gtacgcccgc ttttacacgc cgggcattgc tgacaagaaa
    17821 tatacggaat taccgcaact tgctgtccga tattaaaatt gtgaaagttt tttcctgatt
    17881 caataatctc cccacatatt tcatggccta aaacgcgtgg atagctaaaa aatggttgat
    17941 tgcccgccca ggcatgaata tctgtaccgc aaatcccgac ggttttaatt ttaattaatg
    18001 cttcattatc gcctggcacc ggtctttccc ggactgtcca tactaaattc tttatttcct
    18061 gacaaattaa tactttcatt gtggacatga ttcaccgctc cttttatttt atgactttca
    18121 gatatcgtcg aatttataag agcacctgaa tttacccata aaatatgcgg atgatcacgc
    18181 ataaaaatgt tttaaatcgg tttttaatgc cggactggag ggaagaatga gtcgttcgca
    18241 gaatttacgt cataacgtca taaatcaggt gattgaagat atggcgaggg gcaatattcc
    18301 ttctccgctg ccttcacaaa gtgggctggc agaaatgtat aacatcagtc gcactacggt
    18361 acgccacata ctccagcatt taagcgcatg tggcgtgctc accctggtgg ggaaaaatta
    18421 tgttatcgcc cgcaaacccg aacagaacga tggatttgat tgcatcacgg cctcgctgaa
    18481 agagcaaaac cgtcttttcg agcaggcatt tttcaccatg attaacctgc gccagttacg
    18541 cgcgggagaa agcttttccg aacttcagct ggcgcgagcg gcaggcgtca gtccggtagt
    18601 agtacgagaa tatcttttga aattcggtcg ttataatctt attcaaagcg aaaagcgcgg
    18661 ccagtggagc atgaagcagt tcgatcaatc ctacgccgag cagctgtttg agctacgcga
    18721 aatgctcgaa acacattccc tgcaacactt tctcaacctt cccgatgacg atccccgctg
    18781 gttgcaggcg aaaaccatgc tcgaacgtca caggacattg cgcgacagca ttggcagcaa
    18841 ctttcggatg ttttctcaat tagaccgtga ttttcatgcc atgctgttat ccgctgctga
    18901 caacatcttt tttaatcaat cacttgaaat tatctcggtg atctttcatt ttcactatca
    18961 atgggatgaa agcgatctca agcagcgcaa tatcattgct gttgatgagc atatgaccat
    19021 tctcagcgcc ctgatttgcc gtagcgattt agatgcaata acggcattgc gaaaccatct
    19081 ggataccgcc aaacaatcaa tgattcgttc catcaggcaa catcaccgat aaattaagta
    19141 cagattaaaa acattgattc ttggtttata tcactatgtt gtattaatgc agtattaatc
    19201 caggcgattg ttctgcccat aaggaaatac atagggaata aacgatgctg gaagtattac
    19261 tctcagccat catttaactc cctattactg gtagcgataa aattatcggc tatccatgat
    19321 taacgtttcc agcacatcta attcactgac tatttttgcc agtgtttcat cactgacttc
    19381 ttgccgtgca cgcatctggt aaatagccgc acgcctggca tttaacgctg ccagattaaa
    19441 tgttctttcc agcttcgcgc gctgcatatg taaagaatta tcattttccg acatatcatc
    19501 ttcgctacgc cgcatataac cgataactct ggcccctgtt tccataatca actcgttgtc
    19561 gaggttttct gtactggtgc gtagcaaacg ttgctcagtc ttttgcacac tgagtatggc
    19621 cattttggcc ataaacagat cggcagcttc ctcttctttt tgccggttat cgctgaaaat
    19681 gcccacattt ttccccagta gtaacggcag aataataatg ccgcaaacca gcgaaaagag
    19741 gatcacacct gtggcgagaa ataccagtaa gtaacgcccg ggaaaaggtg tgccatccgg
    19801 cagcaataac gggatagaaa gcacccccgc aagcgtgatg gctccgcgca cccccgcaaa
    19861 ggtcgccagc agcatatgac gcgttttata tccagcaaac agcatcggat gtttgccggg
    19921 tcgtttacgg ctatacagac gcatcagcca aagccagccc aggcgcacaa ccattaacgc
    19981 caggtagatc caaatgacat caaggagcag cgatccggtg tctaccagtg gatcgttttc
    20041 tgcacggatc agtgattccg aaataatgcc cggcagttgt aagcctaaaa tcagaaacac
    20101 catgccgtta aagacatatt caagcatcga ccagctactt ttagctttaa ttctcaggcg
    20161 gataggcgcg tggtggacta cgccaatgcg actggttgtt aaccccgcgg cgacagcggc
    20221 aagaatgcca gataagccaa aatgttccgc aaccagataa acaataaacg gcaacagaag
    20281 catcaataca atctgaatgg aaggttcatc gtgagcatat cgactgatca acagcagccc
    20341 tttgccatat aaccaggtaa cagcaatccc cacggcaata ccgcccaacg caacgacaaa
    20401 aaacgcaatg gttgcgccat gaacgctgaa ttccatcgtt cccatcgtca ctgcaacagc
    20461 aaatttcaac gccaccaggc cggaagcatc attcatcaac gcttcgcctt cgagcacttc
    20521 ctggatgttt ttgctgatac gccctttccc gacaatcccc agcaacgcca cggcatcggt
    20581 aggcgacaac acggcggcta atgcgaaagc agcgatgagc ggcacattcg gtaagagtaa
    20641 atgaatcaca tagccgacac caaaaatagt gattagcacc aacacaagcg ccaggctgat
    20701 aatttcccga ctgtgcttga tcaattcttt aggttgtatt tttgagcttt cagcaaacaa
    20761 caaggggggt ataaacaata agagaaaaag ttcaggattg aattccacat gcagaccaaa
    20821 tatgggctgg gccagtacag cgccagcggc aatctggatc aaaggaagtg gtatctgaac
    20881 aggagacaga cgcgtaagca cgctggttaa tgatactacc aacgtaagaa ttaaaattgt
    20941 taaaagtatt tccatataat aaacaggctt cctcattcat aaataatggg tattattaat
    21001 ttaacttcat aaaaagcgcc ctggttagca cttaacgtta atcattaatc acagcgttca
    21061 ctcacatgct caggaggaaa ccagtgagat gagttttcca gttgataaca aattcaccat
    21121 gtgaatattt actaccttta accgttgtaa cgaatcatcc gttactcttt gatctccttg
    21181 ccgaacaagt tatttagtga atgatggctt tcttttaacg ttagcatgaa ttatacgttc
    21241 agcaaatgta ttaaatcagc actaaataaa tttaatggat accaccattc tgcgtcaaca
    21301 cttagttttt atctgtaaaa atagccgcag ccaaattaat gcttttattt taataaatca
    21361 tcaaccgaat aagagatctt atcagcggta actaaactca tcagtgggtg ttccttgtaa
    21421 cgtcatacat tacataactt taagatatac aatcaaaaaa tgcattaaaa accgattaaa
    21481 agcagcaaag tattacccgg tgcacgaatt tatgacacat ttgcagccat tccgctattc
    21541 gctgactact tttcagtaat gactgctttt tcataaggtt tcactcccga caacaatgtt
    21601 ggagttttat ttccttagca tggagtaagt aaagatgagc aaaataatag cgatgctaat
    21661 tactgtcagt accctttttt taccgtcaat aatccaggca aaacctattt cgataaaagt
    21721 tgcctatgaa aataaccctg gagaacccct ggatgtggtc atgcgttact gggcagacct
    21781 attaaataaa aaaagtaatg gcgaaataac acttgcgctc tatccaagtt cccaattggg
    21841 ctcaaaacag gatgtcaccg agcaagccat gatgggcatg aatgtcataa ccttgagtga
    21901 tgtggccttt cttgccgatt atgaacccga tttgggtatt ttgtttggac cgtatctgac
    21961 tgacgatccg caaaaactgt tcaaaatata tgaaagcgac tggtttaaac aaaaaaatga
    22021 aagtctgaag aaaaagggaa ttcatgtggt aatgaataat tatctctatg gcactcgaca
    22081 gattatttcg aaaaaaccca ttcgcactgt tgacgatctc gcaggcttaa aaattcgtgt
    22141 tcctaataac gtcatgcaaa taaaagccat tcaggcgatg ggggcaacac caactcccat
    22201 gccattaggc gaagtttatc ccgcattaac ccagggcgtt attgacggcg tagaaaaccc
    22261 aatttcagtg ctgcaagggc aaaaactgta tgagcaggca agatatctga caatggttaa
    22321 ctacctgaca aatacctcag tatggatagg aggtgaagcc ttctttagca ccctttcacc
    22381 agaacaactg gagatgatcc accagaccgg ctatgaagcg ggtgtctata gccagaaact
    22441 cactcttgaa cgtgatgccg aaatgttgaa aaccatgcag gcagcgggcg tcgaagtgat
    22501 ctatcccgat actggaccat tccagaagaa agcacgcgaa gtttactctc aattcccgga
    22561 atggacacca ggtctgtacg aaaccattca acagcaactg caataagcat taatgctcag
    22621 cgtcctgcct catctctctt aggcaggaca cgcactaaca ggaggtttca tgcgtctttt
    22681 tggcaaagtg gcagaattta gtgctgcctt tgcgctattc gttctggttg tgatgacaat
    22741 tggagcggta tttatgcgtt atttcatcgg ccaaccgctt caatggacag aagaaatgtc
    22801 gggaatgctg atgatttggg tggtgatgtt aggtggagtg gtggcggaac gtgacagggc
    22861 gcacctgacc attcccttcc tgatggagat gcttccaggc aagctcaggc gtgttattgc
    22921 agtactggtc gccgttctct ccatcgcact gctgctgtat atggcctggc tgggctaccg
    22981 actagccgaa atggcgcagt ttaaagtgac acaacttctg aaggtttcct ggttctggat
    23041 cgatctggcg gtccctgtcg gtgcactggc aacggctatt tataccctct actggttaat
    23101 acacgatata aaacaatctg atagcggaga caaaatgcaa tgagttggtt attgattatt
    23161 ccgctaatga tcgtctgttt gtttctcaac attcgggtct atctggcgat gtttattggc
    23221 atcatcgctt actttttatt cttctccagc gtaccgattg aaattgccgt gcaaagactg
    23281 atcgcgccaa cgcaaagccc atcattgctg gcaattccgt tttttatctt gctcggcaca
    23341 ctaatgagct acaccggcat tgccgaacgc atcctgcaca ttgccaacgt tctggtgggg
    23401 aaaatgcgcg gcggacttgg cgtggcaaac attctcgtca gcaccatgat gggcggcgtc
    23461 agcgcctcta accttgctga cgcggcgatg ctgtcgcgca tgatggtgcc ggagatggag
    23521 cgtaaaggct acaaccgcgc gtttgccgcc gccattaccg ctggtggctc gttagtcacc
    23581 ccgatcatac cgccgggaat agccctgatt atttacggtc tggttgccga cgtctctatc
    23641 ggaaaaatgt ttatcgctgg gctggtacct ggtttgctat gcgcagtcgt tctgatgttt
    23701 accgtctacc tggtcgcccg caaaactaac gctaaaccct cgcgtgaaag ctggcctacg
    23761 ggcaaagagg tggtgttctc gctgggacag gcctggcctg cgctgttcct catttttgct
    23821 gtggtgggtg gtattcgcgc taacattttc accccaacag aagcaggtgc agcagcggtg
    23881 ctaatcgtgc tggcgattgg cttttttatc tatcgtgaaa tgcggatttc tcatgtcgtc
    23941 aaagcattgg gcgaaacggc acgcgcaacc gcatcggtga tgctggtaat catggccagc
    24001 gccgcgttgg gatggatttt ctcaatggaa catgctggtg tcgcagtggc gaattttgtg
    24061 acttcgctga ccgaaaataa atatatgttc ctgttaatta tcaacatatt gctgctgacg
    24121 ctggggatgt ttcttgaagg aaacgccatt ttgctgatcc tggtaccact attaaaaccg
    24181 gtggtcgaac actttggcat cgatccggtg cattttggca tcatcatgat cttcaacctg
    24241 tcgattggcg cattcacgcc accggtaggt accgtgatgc tgctggtatg taacatcacg
    24301 caagtttcgg tgggaaattt ctttaaacaa tccctgcctc tactggcggc tctactatta
    24361 atgctaacgc tggtgacata tatcccggcg atttcactgt tcctggttta ataataaaaa
    24421 cgccggatga aatactcatc cggcgtcatt gcgttaaaac gtttcccagt tctccccgct
    24481 atctgccacg gccattttac gcggcgcagc tggcgtcacg gtttttacca cagccgatgt
    24541 ttcacgctgc tgttgctgct gttgctgaat ccggaacact gccacagctt cggtcaggcg
    24601 actggcctgc tcttccagcg cggcggcggc ggcggcagat tcttccacca gcgcggcgtt
    24661 ctgttgagtt acccggtcca tctcagcaac cgctaagcca acctgatcga taccacggct
    24721 ctgctcatca gaagcagaag caatttcgcc cataatgtcc gtcacgcggg tcacagcact
    24781 gacaatctcc gccattgttt ccccggcgct ttcgaccagc gtagagccaa catccacttt
    24841 tcccaccgag tcttcaatca ggcttttaat ttcacgagcc gcctgagcgc tacgctgggc
    24901 cagattacgc acttctcccg cgaccaccgc aaaaccgcgt ccttgctcac ccgcacgcgc
    24961 agcctcaacc gccgcgttca aagccagaat attggtctgg aaggcaatgc cgtcaattac
    25021 gctgataata tcggcgattt tctgcgaact ggtggagata tcgcgcatag tctgcaccac
    25081 gttatctacc actttgccgc cgcgttgcgc cgtttcagaa gcacttaatg ccagatggct
    25141 ggcctggcgc gcattctcgg cgttctgttt caccgttgcg gtcagttgct ccatgctggc
    25201 tgccgtctct tccagcgaag cggcctgttg ctcggtgcgc gaagagagat cgttattgcc
    25261 ggtagcgatt tcgctggcac cgctatagat ggcattggcc ccgttgcgca catcaccgac
    25321 ggtacgcatc agctctccct gcatatggcg caaactctct gccagttgcc ccatctcatt
    25381 agagccatcc acctcaatcg gtttcaccag atcgccgcct gcaatatgac gaatgctgtc
    25441 aatcaggcga ttcattggcg ctaccagcga ggctttaata ccgaaccaga cggcgaagat
    25501 gaccgccagt acgacgatca tcacgcccac cagaatccac atcgcctggc tgtaggaggc
    25561 attgttatcg ctgacggcga tatcatagag ccgatcgttt tgctccatgt aagccacata
    25621 ctgcttctcg aaaccgtcct gatatccctg ggtcggctga tcaaagaact cgttgatttt
    25681 gcctgcacct aatagttgga tcagctccgc cagcgcattg tgataaatat cgtaattacg
    25741 tttgatctct gccgctgcgg cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc
    25801 cgcccagttt ttttccgcct gtttcagcga aatactggca ctctgcatca gctcagcaac
    25861 ggttgaaccg ctaccaatat tattctgatc catcatgtag cggatacccg cgcggttgag
    25921 ggtgttacgc gtctgcaaca acgcgaccca gctgccattc agcgtggatt gctgctggcg
    25981 aatggtttgt aaaacagtga aattttcttt gtcattcttt aaggcattaa agaacagacc
    26041 gcctgatgtc agttgtaaaa ggccaaaaac ggccaaaacc agcagtaagc tggtcacaat
    26101 tttgatacgt tttaacatgg tttctctttc ctgtggaccg atgcaagatt ttcggcctgg
    26161 aaagcaaaaa ctttatgaaa aggtgtaacc cgccatccat ggaaaaacca ggcgctggtt
    26221 atcagatact aaaaagttat tcattatcgc ccgggatgtc ttcttgtcgg gaattaccca
    26281 gagcaataaa ttcctctaac agatgggtta actgttgcat tttctcggca gtaaactgag
    26341 cttcaatctg ccgataggct tcttcaatct gtgtctgagc gcggttatac agcgcctgcc
    26401 cctctttggt cagcgagata tagagcttcc gctgatcatt gatcggcttt aatcgcaaca
    26461 ctaaaccgtc gcgttccatc cgcgtgagga tcccggtcag gcttgggcgc aaaatgcagg
    26521 cgcgatacgc cagatcgtga aaatccattg atgggctttc cgccagaata cgcacgatgc
    26581 gccactgttg ctcagtcaaa ttatgccgtt taacgattgg gcgaaaataa ctcatcgccg
    26641 cttctcgcgc ctggagcaa
//
LOCUS       DAZGOB010000063        26405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGOB010000063 DAZGOB010000000
VERSION     DAZGOB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.063"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHLIF_002750"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHLIF_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO9606742.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            414..608
                     /gene="bfd"
                     /locus_tag="ACHLIF_002751"
     CDS             414..608
                     /gene="bfd"
                     /locus_tag="ACHLIF_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO9606743.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            681..1157
                     /gene="bfr"
                     /locus_tag="ACHLIF_002752"
     CDS             681..1157
                     /gene="bfr"
                     /locus_tag="ACHLIF_002752"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO9606744.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1154..1621)
                     /locus_tag="ACHLIF_002753"
     CDS             complement(1154..1621)
                     /locus_tag="ACHLIF_002753"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO9606745.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHLIF_002754"
     CDS             2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHLIF_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO9606746.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2344..2973
                     /gene="rplC"
                     /locus_tag="ACHLIF_002755"
     CDS             2344..2973
                     /gene="rplC"
                     /locus_tag="ACHLIF_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO9606747.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2984..3589
                     /gene="rplD"
                     /locus_tag="ACHLIF_002756"
     CDS             2984..3589
                     /gene="rplD"
                     /locus_tag="ACHLIF_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO9606748.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3586..3888
                     /gene="rplW"
                     /locus_tag="ACHLIF_002757"
     CDS             3586..3888
                     /gene="rplW"
                     /locus_tag="ACHLIF_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO9606749.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3906..4727
                     /gene="rplB"
                     /locus_tag="ACHLIF_002758"
     CDS             3906..4727
                     /gene="rplB"
                     /locus_tag="ACHLIF_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO9606750.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHLIF_002759"
     CDS             4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHLIF_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO9606751.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5037..5369
                     /gene="rplV"
                     /locus_tag="ACHLIF_002760"
     CDS             5037..5369
                     /gene="rplV"
                     /locus_tag="ACHLIF_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO9606752.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHLIF_002761"
     CDS             5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHLIF_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO9606753.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6101..6511
                     /gene="rplP"
                     /locus_tag="ACHLIF_002762"
     CDS             6101..6511
                     /gene="rplP"
                     /locus_tag="ACHLIF_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO9606754.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHLIF_002763"
     CDS             6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHLIF_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO9606755.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHLIF_002764"
     CDS             6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHLIF_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO9606756.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7121..7492
                     /gene="rplN"
                     /locus_tag="ACHLIF_002765"
     CDS             7121..7492
                     /gene="rplN"
                     /locus_tag="ACHLIF_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO9606757.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7503..7817
                     /gene="rplX"
                     /locus_tag="ACHLIF_002766"
     CDS             7503..7817
                     /gene="rplX"
                     /locus_tag="ACHLIF_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO9606758.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7832..8371
                     /gene="rplE"
                     /locus_tag="ACHLIF_002767"
     CDS             7832..8371
                     /gene="rplE"
                     /locus_tag="ACHLIF_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO9606759.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHLIF_002768"
     CDS             8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHLIF_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO9606760.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHLIF_002769"
     CDS             8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHLIF_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO9606761.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9130..9663
                     /gene="rplF"
                     /locus_tag="ACHLIF_002770"
     CDS             9130..9663
                     /gene="rplF"
                     /locus_tag="ACHLIF_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO9606762.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9673..10026
                     /gene="rplR"
                     /locus_tag="ACHLIF_002771"
     CDS             9673..10026
                     /gene="rplR"
                     /locus_tag="ACHLIF_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO9606763.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHLIF_002772"
     CDS             10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHLIF_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO9606764.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHLIF_002773"
     CDS             10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHLIF_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO9606765.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10731..11165
                     /gene="rplO"
                     /locus_tag="ACHLIF_002774"
     CDS             10731..11165
                     /gene="rplO"
                     /locus_tag="ACHLIF_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO9606766.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11173..12504
                     /gene="secY"
                     /locus_tag="ACHLIF_002775"
     CDS             11173..12504
                     /gene="secY"
                     /locus_tag="ACHLIF_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO9606767.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHLIF_002776"
     CDS             12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHLIF_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO9606768.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHLIF_002777"
     CDS             12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHLIF_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO9606769.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHLIF_002778"
     CDS             13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHLIF_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO9606770.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHLIF_002779"
     CDS             13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHLIF_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO9606771.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHLIF_002780"
     CDS             14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHLIF_002780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO9606772.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHLIF_002781"
     CDS             15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHLIF_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO9606773.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15761..16129
                     /locus_tag="ACHLIF_002782"
     CDS             15761..16129
                     /locus_tag="ACHLIF_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO9606774.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16140..16565
                     /gene="zntR"
                     /locus_tag="ACHLIF_002783"
     CDS             16140..16565
                     /gene="zntR"
                     /locus_tag="ACHLIF_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO9606775.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16621..16839
                     /gene="arfA"
                     /locus_tag="ACHLIF_002784"
     CDS             16621..16839
                     /gene="arfA"
                     /locus_tag="ACHLIF_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO9606776.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHLIF_002785"
     CDS             complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHLIF_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO9606777.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHLIF_002786"
     CDS             complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHLIF_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO9606778.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHLIF_002787"
     CDS             complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHLIF_002787"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO9606779.1"
                     /translation="MKKQRNLRSMAAQAVEQIVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGVYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAGFYASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHLIF_002788"
     CDS             complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHLIF_002788"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO9606780.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHLIF_002789"
     CDS             complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHLIF_002789"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO9606781.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21710..22834
                     /gene="dprA"
                     /locus_tag="ACHLIF_002790"
     CDS             21710..22834
                     /gene="dprA"
                     /locus_tag="ACHLIF_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO9606782.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22806..23280
                     /gene="smg"
                     /locus_tag="ACHLIF_002791"
                     /pseudo
     CDS             22806..23280
                     /gene="smg"
                     /locus_tag="ACHLIF_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
     gene            23309..23851
                     /locus_tag="ACHLIF_002792"
     CDS             23309..23851
                     /locus_tag="ACHLIF_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO9606783.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23856..24428
                     /gene="tsaC"
                     /locus_tag="ACHLIF_002793"
     CDS             23856..24428
                     /gene="tsaC"
                     /locus_tag="ACHLIF_002793"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO9606784.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24433..25251
                     /gene="aroE"
                     /locus_tag="ACHLIF_002794"
     CDS             24433..25251
                     /gene="aroE"
                     /locus_tag="ACHLIF_002794"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO9606785.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25248..25505
                     /locus_tag="ACHLIF_002795"
     CDS             25248..25505
                     /locus_tag="ACHLIF_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO9606786.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25481..26035)
                     /locus_tag="ACHLIF_002796"
     CDS             complement(25481..26035)
                     /locus_tag="ACHLIF_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO9606787.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6779 a         6277 c         6847 g         6502 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt
      241 aattagtttt gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct
      301 cgaaatattt agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg
      361 taatcataac cattctcatt tacactttgc gcagaatttg aacgggagca atcatgtacg
      421 tttgtttgtg taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc
      481 cgcaatcttt ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca
      541 ttcgcgccgc gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga
      601 ttgcctgaag accacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt
      661 ggaagcggag ggactataaa atgaaaggtg atgttaaaat cataaattat ctcaataaac
      721 tattgggaaa tgagcttgtc gcaatcaatc agtattttct ccatgcccgg atgtttaaaa
      781 actggggcct gatgcgtctc aatgacgtcg aataccatga atccatcgat gagatgaaac
      841 acgctgataa atatatcgag cgtattttgt ttttagaagg aatccccaac ttacaggatt
      901 taggcaaact ggggattggt gaagatgtcg aggagatgct gcaatccgac ctgcgattag
      961 aactggaagg cgcgaaggat ctccgtgagg ctatcgcgta tgccgacagc gttcatgact
     1021 atgttagccg tgatatgatg atcgagatcc ttgccgatga agaaggtcat attgactggc
     1081 tcgaaactga gctggatctc atcggtaaaa tcggtttaca aaattacctg caatcacaaa
     1141 taaaagttaa agattaacgg cctgctagca aactatccca acctacaacg aaacccgcag
     1201 ccgccagaaa tggcccaaac ggcagcgggt tttttaatga ctcttttcct ctcatgagca
     1261 gaccaacaac tacggctccg caagcgaatg aggcagcaag gaaaaccagt cgtggcaaga
     1321 aggtccaggt atgccaggca ccaagggcgg cgagaaactt cacatcacca tagcctaatc
     1381 cttctttatg acgcagtatg cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa
     1441 taatcgcccc ccataacgca tcggctaaac cgtcaggatt acaaacctga gagaacaaaa
     1501 gacctgacca gagtaacggg caggtaaatc tgtcaggtaa caagccatgc tttgcatccc
     1561 ataaaaaaag taggacagat aaacaagcgt acaaaatcag aaagggaagg ttagcgtcca
     1621 taaaacatcc tctgtaattg gagtatcacc atcgaataac tccggatgct cagcaacaga
     1681 gaaatcgctg atttgttaac ctggttccgg tgaaaaacgc tatcttcaca accttacaaa
     1741 tttcgtggaa tacagctcgc aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt
     1801 caggctcaga tttgcgtata atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga
     1861 acctcagtta gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa
     1921 cggttacact ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa
     1981 taattggagc tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga
     2041 tcatcgtctg atcgatcaag caaccgcgga aatcgtcgag actgccaagc gcactggtgc
     2101 gcaggtccgt ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc
     2161 cccgcacgtc aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt
     2221 tgacatcgtt gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc
     2281 cggtgtagac gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa
     2341 acaatgattg gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc
     2401 gtttctatcc cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac
     2461 ctggctaacg atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt
     2521 gtgaccaagc ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg
     2581 tgggaattcc gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa
     2641 ctgtttgctg acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca
     2701 ggtaccgtta agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct
     2761 caccgcgttc cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag
     2821 aaaatggcag gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc
     2881 gttgacgctg agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc
     2941 gacctgatcg ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa
     3001 agacgcgcag agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc
     3061 gctggttcac caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca
     3121 gaagactcgt gctgaagtaa ctggttctgg taaaaaaccg tggcgccaga aaggcaccgg
     3181 ccgtgcgcgt tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc
     3241 tgctcgtccg caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa
     3301 aagcatcctg tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt
     3361 agaagcgccg aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt
     3421 gctgatcatc accggtgagc tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa
     3481 ggttgacgta cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt
     3541 cgtaatgact gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa
     3601 cgtctgctga aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa
     3661 aaatccaaca ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct
     3721 gctgtgcaga aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg
     3781 aaagttaaac gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc
     3841 accctgaaag aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta
     3901 atacaatggc agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag
     3961 tggttaaccc tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca
     4021 aatccggtgg tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca
     4081 agcaggctta ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg
     4141 aacgtcttga gtacgatccg aaccgttccg cgaacatcgc gctggttctg tacaaagacg
     4201 gtgaacgccg ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg
     4261 gcgttgatgc tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt
     4321 ctactgttca taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg
     4381 gtacttacgt tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg
     4441 gtgaaatgcg taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg
     4501 agcatatgct gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga
     4561 ccgttcgcgg taccgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta
     4621 actttggtaa gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca
     4681 gcaacaagcg tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa
     4741 gccatgccac gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta
     4801 gagaaagcgg tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg
     4861 atctttccta acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg
     4921 gtatttgtaa ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact
     4981 tatcgcggcc acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg
     5041 aaactatcgc taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg
     5101 acctgattcg cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga
     5161 aagcggctgt actggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg
     5221 atggcgctga cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca
     5281 tgaagcgcat tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc
     5341 acatcactgt ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt
     5401 acatcctaat ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa
     5461 caccaaagaa ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa
     5521 ggaactggct aaagcgtccg tatctcgtat cgttatcgag cgtccggcta agagcatccg
     5581 tgtaaccatt cacactgctc gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga
     5641 aaaactgcgt aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga
     5701 agttcgtaag cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga
     5761 acgtcgcgtt atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg
     5821 cgctaaaggt attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac
     5881 cgaatggtac cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa
     5941 cacctctgaa gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg
     6001 cgagatcctg ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa
     6061 aaagcagcag cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac
     6121 aaaattccgt aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag
     6181 cttcggcagc ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga
     6241 agcagcacgt cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt
     6301 gttcccggac aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg
     6361 taacgtggag tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg
     6421 tgttccggaa gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa
     6481 aaccaccttt gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc
     6541 gttgaagagc tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg
     6601 caggctgcaa gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc
     6661 gcacgcgtta agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta
     6721 ctctgcaagg tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac
     6781 gttttgtgaa acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac
     6841 atgacgagaa caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc
     6901 tgtccaagac taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca
     6961 gtacactctc tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat
     7021 atccttgaag cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt
     7081 ttcgggtctc agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat
     7141 gctgaacgtc gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg
     7201 ctcgcaccgt cgctacgcag gcgtaggcga catcatcaag atcaccatca aagaagcaat
     7261 tccgcgtggt aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa
     7321 gggtgttcgt cgcccggacg gttctgtcat tcgcttcgat ggtaatgctt gtgttcttct
     7381 gaacaacaac agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct
     7441 tcgtagtgag aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa
     7501 tcatggcagc gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag
     7561 gtaaacgcgg taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca
     7621 acctggttaa gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg
     7681 aaaaagaagc cgctattcag gtttccaacg tagcaatctt caatgcggca accggcaagg
     7741 ctgaccgtgt aggctttaga ttcgaagacg gcaaaaaagt ccgtttcttc aagtctaaca
     7801 gcgaaactat caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga
     7861 cgaagtagtt aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg
     7921 ggtcgagaag atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct
     7981 ggataacgca gcagcagacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc
     8041 acgcaaatct gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac
     8101 tctgcgtggc gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg
     8161 tatccgtgac ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat
     8221 gggtgtccgt gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg
     8281 tggtttggat attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct
     8341 ggctgccttt gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca
     8401 atgaaagcac gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct
     8461 gaactgaaag cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt
     8521 ctcaagctgc agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt
     8581 caaacaggtc gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt
     8641 gaagccgcta tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca
     8701 attgaatcac gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc
     8761 cgtatccgta acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg
     8821 aaagtggcaa tcgccaacgt gctgaaggaa gaaggtttta ttgaagattt taaagttgaa
     8881 ggcgacacca agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta
     8941 gaaagcattc agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg
     9001 ccgaaagtta tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact
     9061 gatcgtgcag cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaatcg
     9121 gaggaaaaaa tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta
     9181 aaaatcaacg gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc
     9241 aacgatgctg ttgaagttaa acatgcagat aataccctga ccttcggtcc gcgtgatggt
     9301 tacgcagacg gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc aatggttatc
     9361 ggtgttaccg aaggcttcac taagaagctg cagctggttg gtgtaggtta ccgtgcagcg
     9421 gttaaaggca atgtgattaa cctgtctctg ggtttctctc atcctgttga ccatcagctg
     9481 cctgcgggta tcactgctga atgtccgact cagactgaaa tcgtgctgaa aggcgctgat
     9541 aagcaggtga tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc tgagccttat
     9601 aaaggcaagg gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag
     9661 taaggtaaca ctatggataa gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc
     9721 aagctccagg agctgggcgc aactcgcctg gtggtacatc gtaccccgcg tcacatttac
     9781 gcacaggtaa ttgcaccgaa cggttctgaa gttctggtag ctgcttctac tgtagaaaaa
     9841 gctatcgctg aacaactgaa gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa
     9901 gctgtcgctg aacgcgctct ggaaaaaggc atcaaagatg tatcctttga ccgttccggg
     9961 ttccaatatc atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc tggccttcag
    10021 ttctaaggta gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa
    10081 agctgatcgc ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca
    10141 cagctctgac tgtagttggc gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc
    10201 gtgaagttcc agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg
    10261 tcgcgctgaa taacggcact ctgcaacacc ctgttaaagg tgttcacacg ggttctcgcg
    10321 tattcatgca gccggcttcc gaaggtaccg gtatcatcgc cggtggtgca atgcgcgccg
    10381 ttctggaagt cgctggggtt cataacgttc tggctaaagc atatggttcc accaacccga
    10441 tcaacgtggt tcgtgcaact attgatggcc tggaaaatat gaattctcca gaaatggtcg
    10501 ctgccaagcg tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg gcaaagacta
    10561 ttaaaattac tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc
    10621 ttggcctggg tctgcgtcgt attggtcaca ccgtagagcg cgaggatact cctgctattc
    10681 gcggtatgat caacgcggtt tccttcatgg ttaaagttga ggagtaagag atgcgtttaa
    10741 atactctgtc tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg
    10801 gttctggcct cggtaaaacc ggtggtcgtg gtcacaaagg tcagaaatct cgttctggcg
    10861 gtggcgtacg tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat
    10921 tcggcttcac ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct gacctggcta
    10981 aagtagaagg cggtgttgta gacctgaaca cgctgaaagc ggctaacatt atcggtatcc
    11041 agatcgagtt cgcgaaagtg atcctggctg gcgaagtaac gactccggta actgttcgtg
    11101 gcctgcgtgt tactaaaggc gctcgtgctg ctatcgaagc tgctggcggt aaaatcgagg
    11161 aataagtagc agatggctaa acaaccggga ttagattttc aaagtgccaa aggtggctta
    11221 ggcgagctga aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt ccgtattggc
    11281 tcttttattc cgatccctgg tattgatgcc gctgtacttg ccaaactgct tgagcaacag
    11341 cgaggcacca tcattgagat gtttaacatg ttctctggtg gtgctctcag ccgtgcttct
    11401 atctttgctc tggggatcat gccgtatatt tcggcgtcga tcattatcca gctgctgacg
    11461 gtagttcacc caacgttggc agaaattaag aaagaagggg agtctggtcg tcgtaagatc
    11521 agccagtaca cccgttacgg tactctggtg ctggcaatat tccagtcgat cggtattgct
    11581 accggtctgc cgaatatgcc tggtatgcaa ggcctggtga ttaacccagg ctttgcattc
    11641 tacttcaccg ctgttgtaag tctggtcaca ggaacgatgt tcctgatgtg gttgggcgaa
    11701 cagattactg aacgaggtat cggcaacggt atttcaatca ttatcttcgc cggtattgtc
    11761 gcgggactcc cgccagccat tgcccatact atcgagcaag cgcgtcaagg cgacctgcac
    11821 ttcctcgtgt tgctgttggt tgcagtatta gtatttgcag tgacgttctt tgttgtattt
    11881 gttgagcgtg gtcaacgccg cattgtggta aactacgcga aacgtcagca aggtcgtcgt
    11941 gtctatgctg cacagagcac acatttaccg ctgaaagtga atatggcggg ggtaatcccg
    12001 gcaatcttcg cttccagtat tattctgttc ccggcgacca tcgcgtcatg gttcgggggc
    12061 ggtactggtt ggaactggct gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt
    12121 tatgtgttac tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt
    12181 ttcaacccgc gtgaaacagc agataacctg aagaagtccg gtgcatttgt accaggaatt
    12241 cgtccgggag agcaaacggc gaagtatatc gataaagtaa tgacccgcct gaccctggtt
    12301 ggtgcgctgt atattacctt tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa
    12361 gtaccgttct acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt
    12421 atggctcaag tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg
    12481 aacctgaaag gctacggccg ataattggtc gcccgagaag ttacggagag taaaaatgaa
    12541 agttcgtgct tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt
    12601 catccgtgtg atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg
    12661 catatttttc ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg
    12721 tctgtgcgtt tccatttgag tatcctgaaa acgggctttt cagcatggaa cgtacatatt
    12781 aaatagtagg agtgcatagt ggcccgtata gcaggcatta acattcctga tcataagcat
    12841 gccgtaatcg cattaacttc gatttatggc gtcggcaaga cccgttctaa agccatcctg
    12901 gctgcagcgg gtatcgctga agatgttaag atcagtgagc tgtctgaagg acaaatcgac
    12961 acgctgcgtg acgaagttgc caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc
    13021 atgagcatca agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt
    13081 ctcccggttc gcggtcagcg taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa
    13141 ccgatcaaga aataatcggg gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa
    13201 acgtgtaaga aaacaagtct ctgacggcgt ggctcatatc catgcttctt tcaacaacac
    13261 catcgtgact atcactgatc gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc
    13321 cggtttccgt ggttctcgca aatccactcc gtttgcagct caggttgcag cagagcgttg
    13381 cgctgacgcc gtgaaagaat acggcatcaa gaatctggaa gttatggtta aaggtccggg
    13441 tccaggccgc gaatctacta ttcgtgctct gaacgccgca ggtttccgca tcactaacat
    13501 tactgatgtg actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata
    13561 acgcctcgtt ttccaggttt gttggagaaa gaaaatggca agatatttgg gtcctaagct
    13621 caagctgagc cgtcgtgagg gcaccgactt attccttaag tctggcgttc gcgcgatcga
    13681 taccaagtgt aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc
    13741 tgactatggt gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga
    13801 gcgtcagttc cgtaactact acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa
    13861 cctgttggct ctgctggaag gtcgtctgga caacgttgta taccgtatgg gcttcggtgc
    13921 cactcgtgca gaagcacgtc agctggttag ccataaagca attatggtaa acggtcgtgt
    13981 tgttaacatc gcttcttatc aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc
    14041 gaagaagcag tctcgcgtga aagccgctct ggagctggct gagcagcgtg aaaagccaac
    14101 ctggctggaa gttgatgctg gcaagatgga aggtacgttt aagcgtaagc cggagcgttc
    14161 tgatctgtct gcggacatta acgaacacct gatcgtcgag ctttactcca agtaaagctt
    14221 agtaccaaag agaggacaca atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg
    14281 ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg
    14341 gctttggcca tactctgggt aacgcactgc gccgtattct gctctcatcg atgccgggtt
    14401 gcgcggtgac cgaggttgag attgatggtg tactacatga gtacagcacc aaagaaggcg
    14461 ttcaggaaga tatcctggaa atcctgctca acctgaaagg gctggcggtg agagttcagg
    14521 gcaaagatga agttattctt accttgaata aatctggcat tggccctgtg actgcagccg
    14581 atatcaccca cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga
    14641 ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc
    14701 cggcttctac ccgaattcat tcggaagaag atgagcgccc aatcggccgt ctgctggtcg
    14761 acgcatgcta cagccctgtg gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac
    14821 agcgtaccga cctggacaag ctggtcatcg aaatggaaac caacggcaca atcgatcctg
    14881 aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca actggaagct ttcgttgact
    14941 tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc agagttcgat ccgatcctgc
    15001 tgcgccctgt tgacgatctg gaattgactg tccgctctgc taactgcctt aaagcagaag
    15061 ctatccacta tatcggtgat ctggtacaac gtaccgaggt tgagctcctt aaaacgccta
    15121 accttggtaa aaaatctctt actgagatta aagacgtgct ggcttcccgt ggactgtctc
    15181 tgggcatgcg cctggaaaac tggccaccgg caagcatcgc tgacgagtaa ccggatcaca
    15241 ggttaaggtt ttactgagaa ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg
    15301 aaccgcaaca gcagccatcg ccaggctatg ttccgcaata tggcaggttc actggttcgt
    15361 catgaaatca tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg
    15421 ctgattactc ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact
    15481 cgtgataacg agatcgtggc aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt
    15541 gccggtggtt acactcgtat tctgaagtgt ggcttccgtg caggcgacaa cgcgccgatg
    15601 gcttacatcg agctggttga tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa
    15661 gcaacgtaaa aaaacccgcc ccggcgggtt tttttatacc cgtagcatcc ccacttatct
    15721 acaatagctg tactcttttt gttcatcccc tggagtattt atgtggttac ttgaccagtg
    15781 ggcagagcgt catatagcag aagcgcaagc gaaaggtgag tttgataacc tggcaggtag
    15841 cggcgaacca ttgatactgg atgatgattc tcatgtgcca ccggaattac gtgcggggta
    15901 tcgcttgctg aagaatgccg gttgcttacc gccagaactt gagcagcgga gggaagcaat
    15961 tcagcttctg gatatcctga gaggtatccg tcacgatgat ccgcaatatc aagaggttag
    16021 ccgtcgattg tcattactgg aattgaagct gcgacaagct ggattgagta ccgatttttt
    16081 acgcggcgat tatgctgaca agttgttgga caaaatcaac gataactagt ggagtatgta
    16141 tgtatcgcat tggtgagctg gcaaaaatgg cggaagtaac acctgacacg attcgttatt
    16201 acgaaaaaca gcagatgatg gagcatgaag tgcgtactga aggcgggttt cgcctgtata
    16261 ccgaaagcga tctccagcga ttgaaattta tccgccatgc cagacaacta ggtttcagtc
    16321 tggagtcgat ccgcgagttg ctgtcgatcc gcatcgatcc tgaacaccat acctgtcagg
    16381 agtcaaaagg cattgtgcag gaaagattgc aggaagtcga agcacggata gccgagttgc
    16441 agagtatgca gcgttccttg caacgcctta acgatgcctg ttgtgggacc gcccatagca
    16501 gtgtttattg ttcgattctt gaagctcttg aacaaggggc gagtggcgtt aagagtggct
    16561 gttgattttt tgcactgaca ggattacact cgcgccgtaa aataaccaac tggagttttt
    16621 atgagccgat atcagcatac taaagggcag ataaaggata atgcgataga agcattacta
    16681 catgatccct tattccgaca gcgagtagag aaaaataaga aagggaaagg cagttacatg
    16741 cgaaaaggaa aacatggcaa tcgggggaac tgggaggcca gtggcaagaa agtaaatcac
    16801 ttttttacca ctggtcttct gctttcaggc gcttgttaag agcggttatt ctgctctttc
    16861 agcaaatcac gaatttctgc cagtaatact tcttctttag ttggtgcagg tgcggcagct
    16921 ggttcttctt ttttccgatt cagtttgttg attagcttaa tcgccataaa gatggcaaag
    16981 gccacaatca gaaaatcaaa gacgttttga atgaagacac cgtaatgcat cacaacagca
    17041 gggatatccc cctgcgcatc gcgtagcgtg acagcaaact gtttaaaatc gatcccgcca
    17101 attaataagc ccagaggagg catgatgata tcggcaacca gtgaagagac aatcttcccg
    17161 aatgccgcac cgataatgac acccaccgcc aaatccacca cgttcccgcg catcgcaaat
    17221 tcgcgaaatt ctttaataat gctcatgtta ttctccctat gaagccgaca accataagtc
    17281 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
    17341 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggttgg
    17401 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg
    17461 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
    17521 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
    17581 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag
    17641 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
    17701 tgtggtgaaa tcgcaatatc gatcacactc ccctgaacca gatccacata agcgcgacgc
    17761 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
    17821 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg
    17881 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
    17941 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
    18001 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgcttatac
    18061 ggtttttcca gtcgctgtaa ttcactcatc accgcacgga tatgctgtga agcggcaata
    18121 aagaacactt catcaccagc ttccacaatg gtcgaacctt gtggacgaat ggggcgatcg
    18181 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
    18241 agtgcgttac caatcagcgg gccgccataa taggctttaa ccacagcgag gctgacttta
    18301 ccctcagcga agttcaccac ctgcaatgcg ccgggatact caatcagtcg gtaaatatta
    18361 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
    18421 agcttatcgg catcgcgcac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
    18481 ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
    18541 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
    18601 tgcccctgca cgactcgcag gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg
    18661 gtatcgacaa cagtgatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
    18721 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
    18781 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg taccaggcag attttgttta
    18841 cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag
    18901 gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt
    18961 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
    19021 tccgggatat cgcgatcgcg acgtaaccat ttaatatccg gatggcgacg aatcacaccg
    19081 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
    19141 gaagggtagc ggccatcacc ttgtttcacg gtcgctttca taccaaggcg ttttaaattg
    19201 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt
    19261 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc cagaatgtgt
    19321 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
    19381 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca
    19441 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccaa caatgcaagc
    19501 cagctgtcgc gggaatgatg cgtacgatta acgcgcagcc acattggcgg acgctggtta
    19561 ttagcttcga cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
    19621 caggaaggat gcagataacg tgcatcactg gcataaaacc cggctaataa ctcttcttgc
    19681 tgacgctgga actggcgtaa tacaccgtta atcagcccct taagttgcgg gcgcttaatt
    19741 gcgacagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
    19801 agttgataca caccaaccat aatcaggtaa tgcacggtcc gctgtttgcc ggtcatcgga
    19861 cgggccatta acttattaat cagccagtct aactgtgaaa gcgtacgcag tacgccaaag
    19921 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
    19981 atgttgctta atgattgccc ttgctcgacg atttgttcaa cggcctgtgc cgccatgcta
    20041 cgtaaattac gttgtttttt cataagtata aaaatgcccg gtaagaccgg gcttagaaga
    20101 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga attcaggagg
    20161 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
    20221 tcaccagtcg caacctgaat gccttgttta ttggcttcaa ggatcgttcc tggtgcagcg
    20281 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggttgtcc ttcaatttcc
    20341 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg cgctgccgaa
    20401 agcgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
    20461 tcgtcctgaa cttctggttt cgccgtgccg tctgctagtt gtttcaacgt ggtgataagc
    20521 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
    20581 atcgggcagg agagcttata aagcatgtca ccggtgtcta aaccaacatc catttgcata
    20641 atggtcacac cagtttctgc atcacccgcc catagtgagc gttggattgg tgcagcaccg
    20701 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
    20761 actgctttag gcagaattaa gccataggcg acgacgacca taacatcagc ctgcagatcg
    20821 gcgaccagtt gctggctttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
    20881 ttttcctcag ccagaacttt aaccgggctg ggcatcagtt ttttaccgcg tcctgccggt
    20941 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga
    21001 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
    21061 gttcttatcc ttaagcccgg gctttcagac gatctagttt ttcaactttc tgacgaatac
    21121 gttgttgttt cagcggtgac agataatcca taaacagttt gccgaccagg tgatccatct
    21181 catgctgaat acagatggct aacagcccct ctgcttccag ttcaaatggt ttaccgtcgc
    21241 ggtcaagggc gcgaatttta actttctctg cgcgcggcac taaagcacgt tgttcaggga
    21301 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
    21361 ttaacaccag ccgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
    21421 tatcaacctg ggttgccgcc aggccaatac cttcttctgc gtacatcgtc tcgaacatat
    21481 catcgacgat acgctgtatt tctgcattca cttcttctac cggtttagca actttgcgaa
    21541 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
    21601 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
    21661 ctgaccaatc gcaaagattg ctaaggctgc ttatggcagg gagataagga tggtcgatac
    21721 agaaatttgg ctgcgtttaa tgagtatcag cagcttgtac ggcgatgata tggtccgtat
    21781 agctcactgg ctggcaaaac agtcgcaaat tgatgcggtt ggattgcagc aaacagggct
    21841 tacattgcgg caggcacaac gctttctttc atttccgcga aagagtatcg aaagctcact
    21901 ttgttggttg gagcaaccca accatcattt aatccctgcg gacagcgaat tttatcctcc
    21961 tcaacttctg gcgacgacag attaccccgg cgcactgttt gttgaaggag aactgcacgc
    22021 gctgcattca tttcagcttg ccgtagtggg gagtcgggcg cattcatggt atggcgagcg
    22081 atggggacgg ttattttgcg aaactctggc gacgcgtgga gtgacaatta cgagtggact
    22141 ggcgcgtgga atcgatggtg tggcgcataa agcggcctta caggtaaatg gcgtcagcat
    22201 tgctgtattg gggaatggac ttaataccat tcatccccgc cgccatgccc gactggctgc
    22261 cagtctgctt gaacatggcg gagctctcgt ctcggaattt cccctcgatg ttccacccct
    22321 tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt ctaagtaaag gtgtactggt
    22381 ggtggaagcg gctttgcgca gtggttcgct ggtgacagca cgttgtgcgc ttgagcaggg
    22441 gcgtgaagtt tttgccttgc caggtccaat agggaatccg ggaagcgaag ggcctcactg
    22501 gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca
    22561 atttggattg cactggttgc cagacgcccc tgaaaattca ttttattcac cagatcagca
    22621 agacgtggca ttgccatttc ctgagctcct ggctaacgta ggagatgagg taacacctgt
    22681 tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag gtagttactc aactactcga
    22741 actggagtta gcaggatgga tcgcagctgt acccggcggc tatgtccgat tgaggagggc
    22801 atgccatgtt cgacgtacta atgtatttgt ttgaaaccta tattcacaca gaagctgagt
    22861 tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga cgcaggattt gatcgagaag
    22921 atatctacaa tgccctgctt tggctggaaa agcttgcgga ttatcaggaa ggactggcag
    22981 aaccgatgca actggcctct gatccactct ccaatgcgta tttatacccc tgaggagtgt
    23041 gaaagactgg atgccagctg tcgtggtttt ctgcttttcc ttgagcaaat tcaggtgctt
    23101 aaccttgaaa cccgtgaaat ggtgatagag cgagtgctcg cgctggatac cgcagagttc
    23161 gacctggaag acctgaaatg ggtaatcctg atggtgttgt tcaatattcc gggctgcgaa
    23221 aatgcgtacc agcaaatgga agaattactc tttgaagtga atgaaggtat gctgcattaa
    23281 tcatttcttt aattcagcat aagttgttat ggcgaaatca gcactgttca cggtgcgtaa
    23341 taatgagtcc tgcccaaagt gcggggctga actggttatt cgatccggga aacacggtcc
    23401 gtttcttgga tgctcacagt atccggcgtg tgactacgtc cgtcctctga aatcttcagc
    23461 ggatggacat attgtcaaag ttctggaggg gcaggtttgc cttgcatgtg gcgcaaatct
    23521 ggtattacgc cagggacgct ttggtatgtt tattggttgc agtaactacc ctgaatgcga
    23581 acataccgaa cttatcgata aaccggacga aacagcaatt acatgccccc aatgccggac
    23641 gggccatctg gtccagcgcc gctcccgtta tggcaaaaca tttcactcct gtgatcgcta
    23701 cccggagtgt caatttgcca ttaacttcaa acccatagct ggagaatgcc ctgagtgtca
    23761 ttatccacta ctcatcgaaa agaaaaccgc gcagggtgta aaacactttt gtgccagtaa
    23821 acaatgtgga aagccggttt cggcggaata ataacgtgaa taataacctg caaggagacg
    23881 ctatcgcagc tgcgatagat gtcctcaatg aagaacgtgt catcgcctat ccaacggaag
    23941 ccgttttcgg tgttgggtgc gatcctgata gcgaaacagc agtgatgaga ctgttggagt
    24001 taaaacagcg tccggttgat aaggggctga ttttaatcgc ggcaaattac gagcagctta
    24061 aaccctatat tgatgacacc atgctgactg atgcgcagcg tgaaaccatt ttttcccgct
    24121 ggccaggtcc tgtcaccttt gtctttcccg cgcctgcgac aacaccgcgc tggttgacgg
    24181 gccgctttga ttcgcttgct gtacgagtca ccgaccatcc gttggtggtt gctttgtgcc
    24241 aggcttatgg taaaccgctg gtttctacca gtgccaactt gagtggattg ccaccttgtc
    24301 gaacagtaga cgaagttcgc gcacaatttg gcgcggcgtt cccggttgtg cctggtgaaa
    24361 cgggggggcg tttaaatcct tcagaaatcc gcgatgccct gacgggtgaa ctgtttcgac
    24421 aggggtaaca taatggaaac ctatgctgtt tttggtaatc cgatagccca cagcaaatcg
    24481 ccattcattc atcagcaatt tgctcagcaa ctgaatattg aacatcccta tgggcgcgtg
    24541 ttggcaccca tcaatgattt catcaacaca ctgaacgctt tctttcgtgc tggtggtaaa
    24601 ggtgcgaatg tgacggtgcc ttttaaagaa gaggcttttg ccagagcgga tgagcttact
    24661 gaacgggcag cgttggctgg tgctgttaat accctcaagc ggttagaaga tggacgcctg
    24721 ctgggtgaca ataccgatgg tataggtttg ttaagcgatc tggaacgtct gtcttttatc
    24781 cgtcctggtt tacgtattct gctcatcggc gctggtggag catctcgcgg cgtactactg
    24841 ccacttcttt ccctggactg tgcggtgaca atcactaatc ggacggtatc ccgcgcggaa
    24901 gagttggcta aattgtttgc gcacactggc agtattcagg cgttgggtat ggacgaactg
    24961 gaaggtcatg agtttgatct cattattaat gcaacatcca gtggcatcag tggtgatatt
    25021 ccggcgatcc cgtcatcgct cattcatcca ggcatttatt gctatgacat gttctatcag
    25081 aaaggaaaaa ctccttttct ggcatggtgt gagcagcgag gctcaaagcg taatgctgat
    25141 ggtttaggaa tgctggtggc acaggcggct catgcctttc ttctctggca cggtgttctg
    25201 cctgacgtag aaccagttat aaagcaattg caggaggaat tgtccgcgtg aatcaggcca
    25261 tccagtttcc ggacagagaa gagtgggacg agaataaaaa atgtgtatgt tttcccgctc
    25321 tcgtgaatgg catgcaactg acatgtgcga tctctggcga gagtctggcg tatcgtttta
    25381 ctggagatac gccagaacag tggttagcga gttttcgtca gcatcgctgg gatctggaag
    25441 aagaagcgga aaacttaatt caggaacaaa gtgaagatga tcaaggctgg gtctggttac
    25501 cctgatccag atattcgtcc ttccatttca cgtaattatt cgcggaatag cgtaacccag
    25561 ccttctcttc atcacttaac gggcggatct gtttgacggg gctaccgaga tacagatatc
    25621 cgctctccag ccgtttattt tgtgggacca gactacccgc accaatcatc acatcatctt
    25681 ctactattgc gccatcaagt aaaattgagc ccatcccaac caaaactcga ttgccaatgg
    25741 tgcagccgtg gagcatcacc ttgtgaccaa cagtgacatc ttcgccaatg gttaatgggt
    25801 tgccatctgg gttgtacgag gatttatgag tgacatgcaa catactgcca tcctggatat
    25861 tggtgcgtgc tccgatctgt acataatgta catctccacg aatcacaacg agcggccaga
    25921 tccccacatc atcagccaga cgaacgtcac caatcacgac actgctatcg tcgatcatta
    25981 cgcgctgacc gatttgtgga aaaagatcgc ggtatgggcg taaaacatca gacatactta
    26041 cctcagcaat aaatgattta ctaatgactt tgggggcatt attggccttg tgcaagtctt
    26101 ttagtatgca aaaaagcacc gttttgtgtg cgaatgcagc aaaaagggtg aaaaaacaac
    26161 aaacagaaaa aaagatcaaa aaagtacttg tgcaaaaaat tgggatccct ataatgcgcc
    26221 tccgttgaga cgacaacgtg aaacacttca caagatggtc gtaacaacga agagaaaaaa
    26281 tcctgaaatt aagggttgac tctgaaagag gaaagcgtaa tatacgccac ctcgcgacag
    26341 tgagctgaaa gccgcgtcgc aactgctctt taacaattta tcagacaatc tgtgtgggca
    26401 ctcga
//
LOCUS       DAZGOB010000064        25034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGOB010000064 DAZGOB010000000
VERSION     DAZGOB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.064"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            53..952
                     /gene="pgrR"
                     /locus_tag="ACHLIF_002797"
     CDS             53..952
                     /gene="pgrR"
                     /locus_tag="ACHLIF_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO9606788.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGHHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1289..2902
                     /gene="mppA"
                     /locus_tag="ACHLIF_002798"
     CDS             1289..2902
                     /gene="mppA"
                     /locus_tag="ACHLIF_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO9606789.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASMENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2953..3984)
                     /gene="ynaI"
                     /locus_tag="ACHLIF_002799"
     CDS             complement(2953..3984)
                     /gene="ynaI"
                     /locus_tag="ACHLIF_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO9606790.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            4228..4485
                     /gene="ynaJ"
                     /locus_tag="ACHLIF_002800"
     CDS             4228..4485
                     /gene="ynaJ"
                     /locus_tag="ACHLIF_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO9606791.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(4535..5485)
                     /gene="uspE"
                     /locus_tag="ACHLIF_002801"
     CDS             complement(4535..5485)
                     /gene="uspE"
                     /locus_tag="ACHLIF_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO9606792.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(5637..6389)
                     /gene="fnr"
                     /locus_tag="ACHLIF_002802"
     CDS             complement(5637..6389)
                     /gene="fnr"
                     /locus_tag="ACHLIF_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO9606793.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(6584..7099)
                     /gene="ogt"
                     /locus_tag="ACHLIF_002803"
     CDS             complement(6584..7099)
                     /gene="ogt"
                     /locus_tag="ACHLIF_002803"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO9606794.1"
                     /translation="MLRLLEEKIATPLGPLWVMCDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(7110..8636)
                     /gene="abgT"
                     /locus_tag="ACHLIF_002804"
     CDS             complement(7110..8636)
                     /gene="abgT"
                     /locus_tag="ACHLIF_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO9606795.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(8673..10118)
                     /gene="abgB"
                     /locus_tag="ACHLIF_002805"
     CDS             complement(8673..10118)
                     /gene="abgB"
                     /locus_tag="ACHLIF_002805"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO9606796.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(10118..11428)
                     /gene="abgA"
                     /locus_tag="ACHLIF_002806"
     CDS             complement(10118..11428)
                     /gene="abgA"
                     /locus_tag="ACHLIF_002806"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO9606797.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQHHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            11604..12512
                     /gene="abgR"
                     /locus_tag="ACHLIF_002807"
     CDS             11604..12512
                     /gene="abgR"
                     /locus_tag="ACHLIF_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9606798.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            12842..13405
                     /gene="smrA"
                     /locus_tag="ACHLIF_002808"
     CDS             12842..13405
                     /gene="smrA"
                     /locus_tag="ACHLIF_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO9606799.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(13426..14658)
                     /gene="dgcM"
                     /locus_tag="ACHLIF_002809"
     CDS             complement(13426..14658)
                     /gene="dgcM"
                     /locus_tag="ACHLIF_002809"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO9606800.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            14852..14899
                     /gene="ydaN"
                     /locus_tag="ACHLIF_002810"
     CDS             14852..14899
                     /gene="ydaN"
                     /locus_tag="ACHLIF_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO9606801.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            14913..15896
                     /gene="zntB"
                     /locus_tag="ACHLIF_002811"
     CDS             14913..15896
                     /gene="zntB"
                     /locus_tag="ACHLIF_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO9606802.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            16171..16344
                     /gene="ynaL"
                     /locus_tag="ACHLIF_002812"
     CDS             16171..16344
                     /gene="ynaL"
                     /locus_tag="ACHLIF_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO9606803.1"
                     /translation="MTTLIYLQIPVPEPIPSDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            16374..17747
                     /gene="dbpA"
                     /locus_tag="ACHLIF_002813"
     CDS             16374..17747
                     /gene="dbpA"
                     /locus_tag="ACHLIF_002813"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO9606804.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(17876..18811)
                     /gene="ttcA"
                     /locus_tag="ACHLIF_002814"
     CDS             complement(17876..18811)
                     /gene="ttcA"
                     /locus_tag="ACHLIF_002814"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO9606805.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(18863..20098)
                     /gene="intR"
                     /locus_tag="ACHLIF_002815"
     CDS             complement(18863..20098)
                     /gene="intR"
                     /locus_tag="ACHLIF_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9606806.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTLISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNMWKFAVYSGLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            complement(20100..20315)
                     /gene="xisR"
                     /locus_tag="ACHLIF_002816"
     CDS             complement(20100..20315)
                     /gene="xisR"
                     /locus_tag="ACHLIF_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFO9606807.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            complement(20415..20603)
                     /locus_tag="ACHLIF_002817"
     CDS             complement(20415..20603)
                     /locus_tag="ACHLIF_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO9606808.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            complement(20641..20790)
                     /gene="ralR"
                     /locus_tag="ACHLIF_002818"
     CDS             complement(20641..20790)
                     /gene="ralR"
                     /locus_tag="ACHLIF_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO9606809.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
     gene            complement(20846..21655)
                     /gene="recT"
                     /locus_tag="ACHLIF_002819"
     CDS             complement(20846..21655)
                     /gene="recT"
                     /locus_tag="ACHLIF_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO9606810.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            complement(21648..24248)
                     /gene="recE"
                     /locus_tag="ACHLIF_002820"
     CDS             complement(21648..24248)
                     /gene="recE"
                     /locus_tag="ACHLIF_002820"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO9606811.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDYEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYLETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNSGKEEE
                     KAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETA
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            complement(24350..24625)
                     /gene="racC"
                     /locus_tag="ACHLIF_002821"
     CDS             complement(24350..24625)
                     /gene="racC"
                     /locus_tag="ACHLIF_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein RacC"
                     /protein_id="HFO9606812.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            complement(24700..24870)
                     /gene="ydaE"
                     /locus_tag="ACHLIF_002822"
     CDS             complement(24700..24870)
                     /gene="ydaE"
                     /locus_tag="ACHLIF_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO9606813.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            complement(24870..>25034)
                     /gene="kilR"
                     /locus_tag="ACHLIF_002823"
     CDS             complement(24870..>25034)
                     /gene="kilR"
                     /locus_tag="ACHLIF_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFO9606814.1"
                     /translation="DYVLHEGRTYIASANNIKKRKLYIRNLTTKTCITDRMIKVFLGR
                     DGLPVKAESW"
BASE COUNT         6235 a         5994 c         6024 g         6781 t
ORIGIN      
        1 tcatgattag acctatcatt taaatgatta atagattgca tggagatact gaatgaagcg
       61 tgaagaaatc gctgatctga tggcgtttgt cgtcgttgca gaggagcgta gcttcactcg
      121 tgcagcagcc cgcctgagca tggcgcagtc agctttaagc cagatagtgc gtcgtataga
      181 agaacgattg ggattgcggc ttctgacgcg aaccacgcgc agcgttgttc caactgaagc
      241 gggcgagcat cttttgtctg ttcttggccc tatgttgcat gacatagatt cagccctggc
      301 atccctgagc gagctgcaga accgcccatc cgggacaata cgtattacta ctgtagaaca
      361 tgcagcaaaa acgatattgt taccagcaat gcgcacattc ctgaaatcgc atcctgaaat
      421 tgatattcag ctcaccattg attatggttt gaccgatgtc gtttctgaac gttttgatgc
      481 aggcgtccgt ctgggtgggg agatggataa agatatgatc gccattcgaa tcgggccaga
      541 tataccaatg gctattgttg gctcaccgga ttatttttct cgccgaagtg ttccaacgtc
      601 agtgtcacaa ttaatagatc atcaggcaat taatttgtat cttcccacat cgggtacagc
      661 aaatcgctgg agattaatac gcggtggacg tgaagttcgt gttcgcatgg aaggtcagct
      721 tttactgaat acgatagacc tgatcattga tgctgcaatt gatgggcatg gattggcgta
      781 tctaccttat gatcaggttg agcgggctat taaagaaaaa aaactgatac gtgttttgga
      841 taaattcaca ccagatttac ccggtcatca cctgtactat ccacaccgtc gacatgctgg
      901 ctcggcattc tcattattta tagataggct gaagtataaa ggtgctgttt agcactactt
      961 gctgatacat taatttaatt tctctcttaa cgtattctca gttcctttca acgttttggt
     1021 tattttttat tcttcgtaca atggcgacag atgctgatta tgataccgaa aactggtttg
     1081 aacgtgcgaa gcctgaacgt agtgttcggt gttctatgtg ctttaccgca ttttggcgtc
     1141 gattatttac actaaatctg atttaatata ttgatacata aaacatttta tgcttcctgt
     1201 gtcacttttt tgatggaagt tgtttgcatt tctttaaggc gaaacaaata attacgcatc
     1261 aattttaatg tcggttagag ggaaacttat gaagcactct gtttcagtca cgtgttgtgc
     1321 gctgttggtc agcagcattt ctctttcgta tgctgcagaa gttccgagcg gcacagtact
     1381 ggcagagaag caggagctgg tgcgccacat taaagatgag cctgcgtcgc tggatcccgc
     1441 taaagccgtg ggcctgccag agattcaggt cattcgcgat ctgtttgaag gactggtgaa
     1501 tcagaacgag aaaggggaga ttgtccccgg cgttgcgact cagtggaaaa gtaatgacaa
     1561 ccgtatctgg acttttaccc tgcgcgataa cgcaaaatgg gcggatggca caccggtaac
     1621 ggcgcaagat tttgtctaca gctggcaacg tctggtggac ccgaaaacgc tatcgccatt
     1681 tgcatggttt gccgcgctgg cgggaatcaa caacgcacag gcgattattg atggtaaagc
     1741 tacgccagac cagcttggcg tcaccgcagt tgatgcccat actttgaaaa ttcagcttga
     1801 taaaccgttg ccgtggtttg tgaatttaac cgctaacttt gccttcttcc cggtgcaaaa
     1861 agccaacgta gaaagcggta aagagtggac gaaacccgga aatctgatcg gcaatggcgc
     1921 ttatgttctt aaagatcgcg tagtcaatga aaaactggtc gtggtaccga atacccatta
     1981 ttgggataac gccaaaacgg tactgcaaaa agtgaccttc ctgccaatta atcaggaatc
     2041 cgcagccact aagcgttacc tcgcggggga tattgatatc accgaatcct tcccgaaaaa
     2101 tatgtatcag aagctgttga aggatattcc ggggcaggtt tatacgccgc cacagctcgg
     2161 gacctattat tatgcgttta acacgcaaaa agggccgacg gcagatcagc gcgttcgcct
     2221 ggcattaagt atgacgatag atcgccgcct gatgaccgaa aaagtgttgg gaacgggcga
     2281 aaagccagcg tggcatttta caccagatgt taccgccgga tttacgccgg aaccttcgcc
     2341 gtttgaacaa atgagtcagg aagaactgaa tgcgcaggca aaaactttgt tgagcgcagc
     2401 tggttatggt ccgcaaaaac cgctgaagct gacgcttttg tataacactt cagaaaacca
     2461 tcaaaaaatt gcgattgctg tagcatcgat gtggaaaaag aaccttggcg tagatgttaa
     2521 attgcaaaat caggaatgga aaacctatat cgatagccgt aacaccggca attttgatgt
     2581 tatccgcgcc tcatgggtgg gggattataa tgaaccctcc actttcctga cattattaac
     2641 gtcaacgcat tcaggaaata tttcacgctt taacaatccg gcatacgaca aagttctggc
     2701 ccaggcatcg atggaaaata ccgttaaagc gcgtaatgcc gattacaacg cggcagaaaa
     2761 aatcctcatg gagcaagcac cgattgcacc aatttatcaa tataccaatg gacgattaat
     2821 caagccgtgg ctgaaaggtt atcccattaa taacccggaa gatgttgcgt acagccggac
     2881 tatgtatatt gtgaagcatt gatgtgatgg gaactggcgt tacccttgtg gataacgcca
     2941 gtgatgtctg atttagcgcc cctgttccgg cggtgtaatg ttatccatat acagcgtctg
     3001 gctggggaag gcaaagtccg cgccgtgtga ctgcacaata tcgataatct tcaaataaac
     3061 gtcttgctgt gcagcaagcc attcagccca taccgtggtt ttggtaaagc aataaaccat
     3121 aatattcaat gacgagtcgg caaactggtt gaaataaacc agtaaggttt gtcgctggtc
     3181 gatggccggg tgatttttca gcatctcacg tacggcttcg acaataacgc ccacttttgc
     3241 cgcatcctca taacgtaaac caatggtcgt ggtaatgcgg cggttggtca ttcgtcctgg
     3301 gttttctacg ctgatcgacg aaaacagcga gttcggtacg tacaatggac gattatcaaa
     3361 ggtcgtaatt ttggtaattc gccagccaat ttccgctact gtaccttcga tatttctgtc
     3421 cggtgaacgg atccagtcgc caatactgaa aggacggtcg aaatagagca tgatcccgga
     3481 aaagaaatta ctcagaatat ctttaccggc cataccgaca gccagaccac caataccacc
     3541 aaaggtcagc aagccagaaa ggctcatgcc gaaatgttcg ccataaagca gaacaagcac
     3601 cacaataatg gtgattttga taatgcgcga cataatccgc gcactggtga tatcgcgacc
     3661 ttttttaatc tgctgttttt caaactgatt aatcagcaaa aatagcttaa tcgtcagaat
     3721 aaccgcaatc agggacgtac agataaaatc gataacgcct ggggtgataa atttgagttt
     3781 atagttttct ataacataat taataatgct accaacagca ctgataatta tggtgtagat
     3841 taaaaattgc accgcatgga ataaaaaacc ttttctttta cgatttccgc ggcgaaacca
     3901 aaagctcatc agaatcaatg ctgcgcagct accgaaaata atgaccagat taagcgcatt
     3961 atttgtaaac agttcagcga tcattgtttt atcaggctcc tccagataat tgtcgtcatg
     4021 ccggaaaccc ctggcggggc tattttaccg cgacaattca ttcagatcat caatagtcag
     4081 ggaaggaagt agcaacatta gctaactaat caatgcgttg attgtaaatc cagctaagag
     4141 gtgaggtttt cagagcagac aacggtgaaa tgtcatggta ttgttacgtt taggtaacaa
     4201 gaaatttgtc tgcacaagga ttacatcatg attatggcga aactgaagtc agcgaaaggg
     4261 aagaaatttc tctttggttt gttggcggtt ttcattattg cggcgtcggt tgtgactcgc
     4321 gcgactatcg gcggcgttat agaacagtac aatattccgc tgtctgagtg gacgacatca
     4381 atgtatgtga ttcagtcatc gatgattttt gtttatagcc tggtctttac tgtgttgctg
     4441 gcaatcccgt tgggaattta tttccttggc ggcgaagagc agtaagtaaa aaataggccc
     4501 gataactcgg gccttgtcag ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt
     4561 caacgggtgt ctgatactgg tcaggtttaa tgaccaacag atcgcagcga agatgatcaa
     4621 tcacctgttc cgccgtgttg ccgaggaatg ctgctgaaat accggtgcgt cctaccgtgc
     4681 ccagaaccac aatccccgcc tgtaagtgct ccgccaaatc aggaatcacc tcttctggca
     4741 gacctttttc tacgtgcgtc atgttttcat taatgccgaa tttctgccgc agagctttca
     4801 ttgccagcaa atgttgccca cgaatggcat cgttataaac gctcggatca aattccggca
     4861 gttcaatcgc gatattaatt ggcgttaccg gataagcgcc aaccagatga acttcggtat
     4921 ggttgacttg ttctgccagt tcgatcgtct ctttgaccag tttttcattg agcgcattat
     4981 gatacggctc ttcactggcg agattcaccg ccaccagcgc cttgcctcct tccggccacg
     5041 gctggtcttt caccatccac accgggcttg ggcatttgcg taacagatgc cagtccgttg
     5101 gcgtaaaaat caccgcttcc agacggtcat gttggtgcgc catttttagc accaaatcgt
     5161 gcccgccgct gatcacttcc tgaatgatgg cttcgaaggg acggttatgc cagaccactt
     5221 taatttcaat gggaacgcca gcattgagat aatattttgc ctgctcgtgg atccaggctg
     5281 tacgctggct gatgacgccc tgacgcatag cggtacgttc gtccggggag agcagggtgg
     5341 tcatttcgta tgagaagtca tagatcggca aaaaggcttt aattttgcca ccaatccgtt
     5401 gatgtaaata aacagctcgc cgcaatgctg gttggtcgtc ctggttagga tcgataacaa
     5461 cgagcatgtt ctgatacata gccatacagg gtctccttac aacaactgtc aacgcagttt
     5521 gtaattaaaa gattaaccca tatctggtga atgaaacagt gatgaacctt ctgccagatc
     5581 aataaatcag aaaaatttaa tgatatgaca gaaggatagt gagttatgcg gaaaaatcag
     5641 gcaacgttac gcgtatgacc agcaagctgg gccagcgcat cgttattttc gatggtgatg
     5701 tatttacctt tgactgccag cataccgctt ttctggaagc gacccagcag acggctgatg
     5761 gtttctaccg tcaggcccag atagttaccg atatcgccac gagtcatcgt caggcggaat
     5821 tcacgagggg agaagccgcg ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc
     5881 agacgttcct cggcattttt cttcgacaac agcaagatca tgtcctgatc gcctttgatt
     5941 tcaccgctca tcagacgcat catctgctga cgcagattcg gcattttacc ggacaaatcg
     6001 tccagcgttt cgaacgggat ttcacatacc atcgaggttt ccagcgcctg cgcgaagctc
     6061 gggtgatggc cgctgccgat ggcgtcaaat cccaccaggt cgccagctaa atggaaacca
     6121 gtgatttgct cgtcgccttg ctcagtgatg gtataacttt taatcgtacc ggagcggatg
     6181 gcataaagcg atttaagttc atcaccagcc ttaaacagcg tctggccttt ctgaataggc
     6241 ttcttccgct caatgatatt atcaagctga tcaagctcat gttcgttgag tgtgaacggg
     6301 atgcaaagct ggctgatgct gcaatcctgg caatggatag cacaaccgcc agactgaatg
     6361 cgccgtataa ttcgcttttc cgggatcata ggtctgctca agccgtaatt gatatttgtc
     6421 aattttaaca tctttttagg gagcaagtaa gtctaagcaa accttaacag cagagaattc
     6481 cgatattaga tgtaaatata tgtctatcta tttgaaaacc cttaagttgt taagggtaac
     6541 tttacataaa agtgtgaaca agctggcaca aattgtttaa tgtttacagc aaaagataac
     6601 cttcgtggcg caataaccac tcttttcgct gaactccgcc tgcatatccg gtcatggtgc
     6661 cgtttcgacc aataacccga tggcaaggta cgacgatgct gatgggattc gatccatttg
     6721 ccgcacctac ggcacgcgcc gcgccagggc ggcccaattg ttcagccagt tggccgtaat
     6781 gcattacctg cccgcagggg atagtgcgta gagttttcca gacttcgcgc tgaaatggcg
     6841 tcccccctgt ggcagtggga agcgtatcaa taatgctaag attaccggca aaataatcac
     6901 gaagcttgtc gcttaaaccg cctgggttgg tggcagaaat gcgctcatag ccttctttac
     6961 gataatggat gtccagcagc tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc
     7021 gcaggcgaaa ttgctcatcg cacatcaccc acagtggacc cagtggcgtg gcaatttttt
     7081 cttcaagtaa tctcagcatc cgttctctct taagacaaac gtgggtaaat ccccggacct
     7141 atcggcagac caacaagata ccacgccagc aacatcagca gccataccac caaaaagata
     7201 agcggatagg gcaagactaa cgaatagtaa gtacccagtt tcgcgtctgg tttgtagcgt
     7261 tgcaggaatc caagaaacag tggaacaaaa ggagataccg gcgctaaagg caatacggat
     7321 gagtcggcaa tacgaaagag gatttgcgca aatgccgggt gaaagccaag tagcataaac
     7381 attggtacga aaatgggggc cagaatcgac cagattgcgg aaccgctggc aataaacatg
     7441 cataagaaag aggaaagcaa cgccagaccg acaaacgccg ggatgccgct aagccctgaa
     7501 ctttcaagta tatcggtcag ccccacggcg atgaatttcc ccatgttgct ccagttaaac
     7561 atggcgacaa actgggcgag gggaaaaacc atcacgataa atcccgccat ctctttcatc
     7621 ggttcaatca ttaaatgcgg taaatccgcc tgacgtcgaa ttgtgcgggt agcgatgcca
     7681 taagccagcg aaaccacaaa gaaaaaaaga atgattagtg gcacgatacc tttaataaag
     7741 ggtgatggca tcacggtgtg attaatcgga tcgcgcaata tcccgttttc cgggatcacc
     7801 atcagcgcga tcgcagcaat aaaaagtagc gatacgacac ctgctatgcg taaaccaaaa
     7861 cgctgacttt cggtcaatgt ctgcagtttc tcatcgctgt ttccctgcca ttgacctaac
     7921 cgtggctcga tgattttgtc ggttatcaag ccgccaacaa tcgtcagtac gactacggag
     7981 ctggccataa aataccagtt atcaattaca ctgacgtgca tttgcggatt gaacgcagcc
     8041 gccgcttccg tgctgatccc cgacagcaac acgtcggttg tgacaatcag taaattagcc
     8101 gtaaagccgc aacctacgcc tgcaatggca gccagtaaac ctgcaactgg atgcctgccc
     8161 accgccagaa aaatcagcgc acccatcggt ggcatgatca ctaacgccgc atcggaagaa
     8221 atgtggctga aaaaagcaat aaacagcacc atataactgg cgtagcgggc attaacatgc
     8281 gatgccattt taaccattag cgctggcagt aagccgacgc gctccgctag gccggcacct
     8341 aaaaccagcg ccaggatcgc tccaagtgga gcaaaaccgc taaagttttt aatcacattg
     8401 ggtaaaaacc agtgtaatcc ttccacactg agcaggtttt tcaccaccac cggcgtacca
     8461 tcggtcgggt ttttcgcact gacgccaaag gctgacaaaa ttgccgtcgt caccatgagt
     8521 acgattatca aatagataaa gagcaaaaaa ggatggggaa ccttgttacc aattctttcg
     8581 acccagccat agagcttccc ggattgggag gacgacggta tggatgacat actcatgggc
     8641 attcctcggt tgttgtgttt gcgttgttgt tgttatttta aaggtgacgg tgtcacgttt
     8701 ttcgggatag ggcagtgata cggttgcgtg tccgtaactt gctgatgctc ttgttggcat
     8761 tcttgcaata gccctgaatc aatgaagaga ttgactgtgg ttgctgccat agttttcgcc
     8821 gccagcagca ttcctttatg agcaatagat gttcgcccct ggctaaccag ttgccacgta
     8881 tgtagcggtg taccgacggc aaaacagggg ctgaaacact gggcaacagg cagtttccaa
     8941 ctgacgtcgc cgacatcagt cgatgccgca agcacgttat cggtggcggc atatggagcg
     9001 acctcattcg ccagtaccgt ttcacgatga cgtagtgcaa aagccttgcc gttttcgcca
     9061 ccggttgcgg cgatattatt cagactgttt tgccgatcgt tgggggtgag cgtagcctga
     9121 atttgtttcg caaaagccag ttcttcggag ttccattccg gggtaccaaa atgggatagg
     9181 gcatggtaca tggcattttc taaggtgcga ttcgggagat aactggaaca ggctttgtcg
     9241 aagcggcatt caaccgtggt ttcggtcatc aatgccgcac cttcggcgat tttggcgacc
     9301 cgatcataaa tatgctgcac gtcggtcatt tcgggggcgc ggataagata aagcacttct
     9361 gcctgcgcct ggaccacgtt gggcgagatc ccgccgctat ttgtgatggc atagtgtacg
     9421 cgcgcttttt caataatatg ttcgttgagg aagttggtgc cagtggtcat caacgttacg
     9481 gcatcaaggg cgctgcgtcc caaatgaggg gaattcgcgg catgtgctgc gatcccttta
     9541 aagcaccatg atgcctgaat gtttgccagc gtgcgggtat tgaacatgcc ggcaaaggct
     9601 tccgggtgcc aggtgagtgc cgcatccaca tcatcaaata ccccctcgcg gaccatgaac
     9661 gttttacccg agccgccttc ttcgccagga caaccataaa agcgcactgt gccgccttgc
     9721 ccatattgtt ccagccattt cttgacggct attgcagcgg caaaggcggc ggttcccagc
     9781 aaattgtgtc cgcaaccgtg accattttca ccgggcgtcg cggatgtagg ttgcgcgcaa
     9841 ccagcttgct gacttaaacc tgccagggcg tcatattctc ccagcagggc gataaccggt
     9901 ttgccttgac caaacgaagc aataaatgca tttgggatat tgcctacgtt gcgggtaacg
     9961 gtgaagcttg cagattccag cgccgaagcc agatgctccg ctgaccagaa ctcttcaaaa
    10021 cgtgtttctg gatgatccca gatttgatcg gcaatatcgg tataacgttg gcgatcggct
    10081 tcaatcgcat cgtcgataaa acgatagatt tcctgcatca gatacctcgc gtccagggaa
    10141 aattgagcgc ggtgcgcgcc agcgtttcga cggcaatagc gagaacctgc tcgtcaaaat
    10201 cgaatttttc gttgtgatga cctgccgcca gctgtgtgcc aaacaccacg taggaggctt
    10261 gcccttgatg gtgctgcacg cgggccatca ttaatgtggc atcttcggaa cccgcaggcg
    10321 cttcaacacg ttcaatggcc tgattgaccc ccgcgacctg agccgcctga ctttgcaacc
    10381 atgcgaccca ttgcggcgaa ggagaactgg cggtagctgc acccatcagg cgagtttcaa
    10441 cgccgacacc atacatggtt gctgcgccct gaatcgcttg ttgtgcacgg tcaaaaacat
    10501 attgattaat gacgtcgctg gccccgcgtg tttccacttt cagcaacgcc gaggcaggaa
    10561 caacgttacg gccgcttcct gcctgcataa cgcccacgtt tactctggaa gctccttcgc
    10621 tgtgcggggc gattgcatgc agtgcaagag ttgcttgtgc tgccgccaac aaggcattgt
    10681 gaccgtcttc tggttttgcg cctgcgtgag cggcggtccc ggtgaagtgc gcgtcaaatt
    10741 tggtggttgc cataaaatta tcactgccgc acaccacggt gcccgcaggt acgccagtgc
    10801 caatgtgcac ggcagtaaaa taatcaacat catctacgac acctgcatcg accatcgccc
    10861 gcgcgccacg cgtaccttcc tctgcaggct gaaaaatcag tttgatgacg ccatgtagtc
    10921 cggactcgaa ttgtttaagg gtatgcgcca gcccaagccc aatggcggta tgcccatcat
    10981 gaccacaggc atgcatcatt ccggcgttac atgacgcaaa accgtcgcgg taggggcgat
    11041 ggctgacatc ctgctcttca ctgagatcca gcgcgtccat atcgacacgg aaagccataa
    11101 ccggaccggg gcgaccggta tccagggtgg cgacgatacc cgtgaaacca ccttcaaaaa
    11161 ccgcaatcca ttgtgctagc gcaccctgtt gacgagcgcg ctcgaattcg cgttgtagag
    11221 tgagttcatc aggtagtccc atccggctac tttcattaac tacttcgcgg cccagcgcca
    11281 gtgaatagcc gagctgctgc aattcttccg caacaagggt ggcagtgcgg aattccaccc
    11341 agccagactc tgcatagtga tgaaaatcac gtcgccagtg cgataatttt ggggcaagcg
    11401 aattaacaaa ttgattcaaa gactccataa cctttcccgt catcagtaaa aagtgtgacc
    11461 cggttcacgt agcgatagtt tttacttatc actaactgat ttttcacagt tttaaccgtt
    11521 cataaattac cctgacacaa tcatctgcat taaagtagat gccagtttct ttggtctgat
    11581 aaataacggt tatcggtggc gtcatggctt ttcaggtaaa aattcatcaa attcgggctt
    11641 ttgttgaagt ggctcgtcag ggcagcattc gcggagcgag ccgaatgttg aatatgtcgc
    11701 aaccggcact gagtaaatct attcaggagc tggaagaagg attagcggcg caactctttt
    11761 ttcgccgtag taaaggcgtg acgttaactg atgccggtga aagtttttat cagcacgcca
    11821 gtcttattct tgaagagctg cgcgcagcgc aagaggaaat tcgccaacga caagggcaac
    11881 tggcagggca gattaatatc ggcatggggg ccagtatttc ccgcagtctg atgccagctg
    11941 tcatatctcg ttttcatcag cagcatccgc aggtaaaagt acgcattatg gaagggcaac
    12001 tggtgtcgat gattaatgaa ttgcgtcagg gagaattgga tttcaccatc aatacctatt
    12061 atcagggacc gtacgaccac gaatttactt ttgagaaatt actggaaaag caattcgcga
    12121 tcttttgccg cccgggacac cccgccattg gtgcccgttc gatcaaacag ttactggatt
    12181 acagctggac aatgccgacg ccacacggca gttactacaa acagttgagt gaattgcttg
    12241 acgatcaggc gcaaacgcca caggtcggtg tagtctgcga gacgttctca gcctgtatca
    12301 gtctggtggc aaaaagcgat tttctcagca tactgcctga agaaatgggc tgcgatccct
    12361 tgcacggaca ggggctggtg atgttgccgg ttagcgaaat tttaccgaaa gcggcctatt
    12421 atttgattca gcggcgtgat agtcgccaga caccactgac cgcgtcatta atcacgcaat
    12481 tccggcgaga atgcggctat ctgcaaagtt aaaactgcat aaaaaaatag agtctgtcga
    12541 catccgccag actctacagt acacacagca gtgcatccgc gtcttaagtc cggcattgtc
    12601 tcctctgcgc cggtgactgt gttataacgg ttttaacctt tagttgccaa ttttcttcag
    12661 tgacagattt cacgaaaata taagttacat tattaatatc gtgaatgaat aatcatgcat
    12721 aagcattttg cttaaaatat cggcaatatt tggaacttat tactggaatt ttgggtaaga
    12781 cgttgttgga ccgacccggt ctggttatca tatcgcgctc ttaattgcgg gaggatgtaa
    12841 catgaacctt gacgacaaat cgctgtttct tgacgccatg gaagatgtcc agccgctgaa
    12901 acgtgctacc gatgtccact ggcatccaac gcgtaaccaa cgtgcgccgc agcgtatcga
    12961 cacgctgcag cttgataatt tcctcaccac cggatttctc gacatcatcc cactaagtca
    13021 gccgctggag tttcggcggg aagggttgca acatggggtg ctggataagc tgcgcagtgg
    13081 taaatatccg caacaggcga gcctgaatct tttgcgccag ccggtggaag agtgccgcaa
    13141 aatgatgttc agtttcattc aacaagccat ggctgatggt ttgcgtaacg tgctgattat
    13201 tcatggcaaa ggacgggatg ataaatcgca tgccaatatt gtccgcagct atgtggcgcg
    13261 ctggctgacc gaatttgatg atgttcaggc atattgcacc gcgctaccgc atcatggcgg
    13321 cagcggggcg tgttacgtcg cactacgtaa aacggcgcag gcgaagcaag aaaactggga
    13381 gcgccacgct aagcgcagcc gttgatctcg agacgcatcc gcggcttatg ccgccagcac
    13441 gcggttgcgt ccatcatttt tcgcccgata caaagcatca tcaacgcgtt taaacagttc
    13501 atcgatgctt tcatttcctt cgtgatgcgc cacaccaatg ctgacggtaa aacgtggtaa
    13561 gcccgaaata ctcacttttg ccacgcttac acggatagtt tcagccagcg aaagcgcggt
    13621 atccagtggg gttcttggta gcaataagac aaactcttcg cctccccaac gaaacaccaa
    13681 atcgcctttg cgagcgcaac tttcgagggt gcgggcgagg gcgcataaca cctcatcacc
    13741 tttagaatgc ccatagagat cgttaatgtg tttaaaacga tcggtgtcga tgagcaacaa
    13801 gctgtaatcc tgggcgatgg cgaggtgctg catttggcct ggctcggtaa tgtgataaaa
    13861 ctgtcgccga ttcagtaatc cggtcatcgc gtcatggtga gcagcatgtt ccagctgctc
    13921 ctccagccgt ttttgctcag taatatcatg cacaatacat aacatgagct tgtcgccata
    13981 aatttcaatc ggcccggcat aggtctgcac atgacgagta gatccatccg ccagtttatg
    14041 aacaaaattc aaaggtttat gaccgccggg taaatgcgaa atttcatgca tgataggcat
    14101 gacgcgacgc ccgagcatat taatttccca ggtatgtttc tggcacatcg tttcatggtt
    14161 ataaccatag aaattgagcg cggcgaggtt agcatcgacg atttggccat ctcgtgaggg
    14221 gtcaatcaac aacattggtg cagagttagt cagaaaaaag cgcgcataaa aaccttgttt
    14281 tttgcgctga taatttgccg agcgactggc ttttaaaccc agcgttgccg gcgcttcgat
    14341 accttcgaaa ataatcaccg gttctgtttc tgtcagctct cgcaaaacaa gccgacagct
    14401 taatgctgtt tcctcttctt tacgctgaac agtgaggatt tcgataatat cgtgttggtt
    14461 ttgcagatcg gagaggtatt tcggcagttc tttttgtgag gagacggaat agggtccggt
    14521 tcgtagctga ctaaacgtga ggtcttgcat caacagtcgc gccgcgctat tggcataaat
    14581 taactgttcc tcaaagggcg aaacaatcca gacaggactg gtgagtaagt ccagggtatt
    14641 gaagttgtgc gtaatcattg agatcccgtt atttttatca atttttgttg ctatccgatc
    14701 gcaaaaaagc cacgtcatat gatcagataa ttctgataat gatagacgct atttaacact
    14761 tcacacggtt tgtatacgga aaagcatttt gctttttgta ttcaatttag acagaatttt
    14821 attaatcatt tcagggtaat ggggtgatga gatgttgcgt aacagggcca gaaggctaga
    14881 ctacaaaata atgcgttgat gatggaggca ctgtggaagc gattaaggga tcggacgtta
    14941 atgtcccgga tgcagtattt gcctggatgc tggatggtag aggcggcgtt aaaccgctgg
    15001 aaaatacaga tgtgattgat gaagcgcatc cctgctggct ccaccttaat tatgtacacc
    15061 atgatagcgc ccaatggctg gcgacaacac cgctgcttcc caataacgta cgtgatgcgc
    15121 tggcgggcga gagcacacgg ccccgagtca gccgtctcgg tgaaggcacg cttattacat
    15181 tgcgctgtat aaacggcagc accgatgaac gccccgatca actggtcgcc atgcgtgtat
    15241 atatggacgg gcggttaatt gtttcgaccc gacaacgcaa agtgctggcg ctggacgatg
    15301 tggtgagcga tctggaagag ggcacgggtc cgaccgattg cggagggtgg ctggtggatg
    15361 tgtgcgatgc gttgaccgat cattccagcg aatttatcga gcagctgcac gataaaatta
    15421 tcgaccttga agataatctc cttgatcagc aaattccacc gcgtggattc ctggctctgc
    15481 tgcgcaaaca attaatcgtg atgcgtcgct atatggcacc gcaacgtgat gtctatgctc
    15541 gtcttgccag tgaacgtttg ccgtggatga gcgatgacca acgccgtcgg atgcaggata
    15601 ttgccgatcg ccttgggcgc ggccttgacg aaatcgacgc ctgtatagca cggactggtg
    15661 tgatggcgga tgaaatcgct caggtgatgc aggaaaattt agctcgtcgt acctatacaa
    15721 tgtcattgat ggcaatggtc tttttaccca gtacctttct gacagggtta tttggcgtca
    15781 accttggtgg gatccctggc ggcgggtggc aattcggatt ttcaattttt tgtattctgt
    15841 tagttgttct tattggtggt gttgctttat ggttgcaccg tagtaaatgg ttgtaacaaa
    15901 agcaattttt ccggctgtct gtatacaaaa acgccgcaaa gtttgagcga agtcaataaa
    15961 ctctctaccc attcagggca atatctccat tgcaggtgaa tgcaacgtca agcgatgggc
    16021 gttgcgctcc atattgtctt acttcctttt ttgaattact gcatagcaca attgattcgt
    16081 acgacgccga ctttgatgag tcggcttttt tttgcctgtt atttatcagc gtctaccctt
    16141 taagagtcca cccaatgacc ggagggaaat atgacgacac ttatttattt gcaaattcct
    16201 gtccctgaac cgattcctag cgatcctgtt ccagtgcccg atccgatccc tcgcccgcaa
    16261 cccatgcctg acccaccacc cgatgaagaa ccgattaaat tgtcgcatcg tgagcgtaga
    16321 tctgcgagga tacgcgcctg ctaactttgc gtcgatgacc acgagaatag attgtgaccg
    16381 ctttttctac cctgaatgtt ttgcctcccg cccaactcac gaaccttaat gagttgggtt
    16441 atttaaccat gacgccggtg caggccgccg cgcttccggc gatccttgcc ggaaaagatg
    16501 ttcgcgtgca ggcgaaaacc ggcagcggca aaacggcggc ttttggcctc ggcttgttac
    16561 agcaaattga tgcgtcgcta tttcaaaccc aggctttagt gctgtgtcct acgcgtgaac
    16621 tggcggatca ggtggcaggt gaattgcgtc ggctggcgcg ttttctgcca aataccaaaa
    16681 ttttgacgtt gtgcggtggt caaccgttcg gtatgcagcg tgattcgttg caacatgcgc
    16741 cgcatattat cgtggcaacg ccgggtcgtt tgctggatca cttgcaaaaa ggcacggtat
    16801 cactggatgc gctgaatact ttggtgatgg atgaggccga ccgcatgctg gatatgggat
    16861 ttagcgatgc cattgatgat gtcatccgtt ttgcgcctgc atctcgacag acgcttctgt
    16921 tttcggcaac ctggccggaa gccatcgccg caatcagcgg acgagtacaa cgcgatcctt
    16981 tggcgattga aattgactca acagatgctt tgccacccat tgaacaacaa ttttatgaga
    17041 catccagcaa aggcaaaatc cctctgttgc aacggttatt aagcttgcat cagccatcct
    17101 cttgcgtggt gttttgcaat accaaaaaag attgccaggc tgtctgcgac gcgctgaatg
    17161 aagtagggca aagtgcattg tcgttacacg gcgatctgga gcaacgcgat cgcgatcaga
    17221 ctctggtacg ttttgctaac ggtagcgccc gtgtactggt tgctactgat gttgctgcgc
    17281 gtggtctgga tattaaatcg cttgagctgg tggtgaactt tgagctggcg tgggaccctg
    17341 aagttcatgt acatcgcatc ggtcgtacag ctcgtgcagg aaatagcggt ctggcgatca
    17401 gtttctgtgc tccggaagaa gcacagcggg ccaatatcat ttctgacatg ttgcagataa
    17461 aacttaactg gcaaacgccg ccagctaata gttccattgt gccgctggaa gcagaaatgg
    17521 caacgttgtg tatcgatggc gggaaaaaag ccaaaatgcg cccgggtgat gtattaggtg
    17581 cactgacagg agatatcggg cttgatggcg cagatattgg caaaatcgcc gtgcatccgg
    17641 cgcatgtcta tgtcgcggtc cgtcaggctg ttgctcataa agcatggaaa cagttacagg
    17701 gcgggaagat taaaggaaaa acgtgccggg tgcggttatt aaaataatga aatgttgaat
    17761 tgccgggtgc aagagtaaac atcttattcg ggattgccgg atgcgacgct ggccgcgtct
    17821 tatccggcct ccataagagt agcccgatac gcttgcgcat cgggcgctat cctggttatt
    17881 tcacttcaac cacattcagt cgtaactcat ccaactgatt ttcatcttct tctggctgcc
    17941 agcccgccgg ttgtagtggg atctcttcgc gatcaaacgc cagatcaccc ccgttaacca
    18001 cttcagaacc gtgggtaatg cctttaaaat cgaacaggtt ggtatcgcac agatgcgacg
    18061 gcaccacatt ctgcatcgcg ctgaacatcg tctcgatacg ccctggataa cgtttatccc
    18121 agtcacgcaa catgtcagca atcacctgac gttgcaggtt aggctgtgaa ccgcacaggt
    18181 tgcacggaat aatcgggaac gcttttgcat cggcaaatcg ctgaatatct ttctcgcggc
    18241 agtaggccag cggacgaata acgatatgtt tgccatcatc gctcatcagt ttcggaggca
    18301 tacctttcat cttgccgccg tagaacatat ttaagaacaa cgtttgcagg atatcgtcac
    18361 gatggtgacc caacgcgatc ttcgtcgccc ccagttccgt tgcggtacga taaaggatac
    18421 cgcgacgaag gcgagaacac agtgagcaag tggttttgcc ctctggaatc ttctctttca
    18481 cgataccgta agtattctct tcaacaatct tgtactcaac gcccagcttt tcaagatact
    18541 cgggcagaac gtgttccggg aagcccggtt gcttttgatc gaggttaaca gccaccagcg
    18601 aaaaattgat tggcgcgctt tgctgcaaat tgcgcagaat ctccagcatg gtatagctgt
    18661 ctttaccccc ggagaggcaa accatgatgc gatcgccttc ttcaatcata ttgaagtcag
    18721 caatggcttc gcccacgtta cgacgcagac gtttttgtaa tttgttcagg ttgtattgtt
    18781 ctttctttgt aatttgttga ttttcttgca ttatttcagt tctctggtac taaatggggc
    18841 aaattggggg caaactttgc aactatgata accgcgcatt caacatggct atctgttcgt
    18901 cgttcatgtc atcaatccac ataccgtaaa tttcatacac catctgcgca gtttcatgcc
    18961 ccatttggct ggctataaat gccgggttcg ctcctgccgt caacagccag caggcaaaag
    19021 tatgccgcgt atggtacgga ttacggcggc gaataccagc acgttttact gctgcattcc
    19081 accttgcccc caaactgctt accgagtaat aaggtttttg ttttccgtta cacaccctgg
    19141 gcatgaaaac aaaatgcagt ttttgctttt cggttctgcc gtactcccga tgataaaagg
    19201 tgatttcgct tttgcgatga tgcccggtca gtttgtattg ctccttcagt gcttcaagag
    19261 caggctgcag tagtgttact gtccggatcc cggcatttgt ttttggggga ccgaacatat
    19321 caagtatcgt caggtttctt ctgacattca ctattccctt ttcgagatcc acatcctccc
    19381 acgccagagc tgccagttcc ccgtgacgaa gtcctgagta aactgcaaat ttccacatgt
    19441 tctggctctg tcctttttca ctttccatta atgcattgaa ttctgtttta gataacggat
    19501 caggctttat tctgtttcgc tgtaattttt ttactccttc aaatggtttg gttgatataa
    19561 atcccgactg atacgcaaaa cgtaacagcg aacagagcag ggcgatatag ttatcaactg
    19621 tgcgcacggt tcttcctttt ttgttggatc ttggattatc caggtaaagc gtttctccat
    19681 gcagcagttc attccggtag tttaagatat cgctataacg aatatgtgat atcagggtac
    19741 tttcacaaat tattattctg agtgttttta attgtgattt cgttttcttc attgtgtttg
    19801 ttgttaactc tgtctcttta atttttgtcc agatatcaca aagctctccg aacgttttta
    19861 tgactctcgt tgtcaccatt tttgccccag tgctggactg gggaaaacgt cttaaatact
    19921 caaattcacc ggagtttatt tcatgaacta tcagcgctct taaatttccg gcctttttaa
    19981 tattactgtt tgtaatctcc cagcctttta atgtttcccg acatcgtttt cctcgaaaca
    20041 tgaaccagat gcgaatgtat ctacctctaa tctcgacacc tgttggtaat ttagacatat
    20101 catgagtctt tgataaactg atttatcttt ggatagttgt accagataat ccctcgtttg
    20161 ctgtctggct tacctaaagg agatactcgt ttgaagtgga agccctccac ccaacagttc
    20221 tggcggtatg cttcaatttg tctggccccc agaccagtgc gaagcatcag gccgtattca
    20281 accatccact cttcattaaa gattacttgt gccatcgcat cacctctggc aggcgccaat
    20341 gttagactga aattgacgcc cgatgttgat tattaataat cagctatgaa gttttaattt
    20401 gaatacaatg caattcacga ggactgaagt ttctcgcaat taaaatttat cagttttact
    20461 ttctgctctc tggaaacgcc tgcttctttt ttacctgaga gcattttttc gcattctgat
    20521 ttcgttagtt tagattttga atatcttgtc cagttagtag gagtgccacc ttccttttca
    20581 atagtggcgg taattttata catgaacacc tccattatta tttccagtgg ttcgtttatt
    20641 tcatcgttcg agtgcttctt tttcacttcc accataaccg gttcgggatt cgcatccgtt
    20701 acacttcgct cggtaatatc ctgaaatggc tttcaccgtt actgatggac aaccacaaaa
    20761 tggacatggt ttaacattgt catatctcat aatttttctc ataaaaatat ttcaagttgg
    20821 cggtgcatta caccgccagg ctgaattatt cctctgaatt atcgattaca ctgtattccc
    20881 cggttaatac agaggaatct gcaggatcga ttgtcagtgg ttccttttca tccattgata
    20941 ctgcacgctg gatctcaatt gatacgggca gatatttgaa caggcgacga atagccgttt
    21001 tctttgccat ttcttcccag tgagttaccc acggcccgtt attaccagct ttactctggc
    21061 tgcgcaccag ctcaatctgt ttgcgcgtca taacttcaaa ctgagtacct ccgtctttca
    21121 gtcttgcgac agcatagacg tgggtaacag gggcatcttc gttttctcct gggcggtgta
    21181 ttaacttttc atcaaggcca aattcgaaat taaactcgtc accttcacgg acaacacggg
    21241 ctgacaggct ggcgatttga cctgaacggc gagccagatc aatcatgccg cgatagccaa
    21301 tgattagctg aacgtttttt ttaccgctct tttcgttttt attaccaaaa ggcagtaaat
    21361 atgcatgacc gagggcgcta ccgggctcaa gtccgagctg tgaacactgt acgattgcac
    21421 tgacaaaact catagtgtca cagtttccta acgccggaac tttacgaatt tctgtggtgg
    21481 cgatacggat catacgttca gccgtcatat ggcgtggaag agctgctgcc agttgctctt
    21541 tcattgatgg ctggttaata aaactaatca cgtcgttatt tttaattgct gctggtgcac
    21601 ggtttccctg agttttttgc agatcggctt ttgcgattgg tggttgctta gtcatttgca
    21661 tattccttag cccagcgggg cagtgataac gtcttaatag ctggccattc atcggtattg
    21721 aggcagtcag ccagggttcg cagattgcgg tgatattcca gctgacctgc cagttttgct
    21781 tcttcgccca tcatgaaaat ttcaaccgga taacgtccgc attcaatagt tgtgctggca
    21841 accagaaaaa cgaaagttgg ctgcactcca aactgtgctt cataaccgtc actgtagaat
    21901 gcatcctgaa cgtgatagcg gtagtcgtaa taagcggttt tgaatcgttg aatatccgcc
    21961 gtagttttca cgtccatgat ccagtgaaat tcagggataa ttttgtccgg acggcaccga
    22021 cacaaaattg ctgtttcagg atcttcccag taaattgatg attcagcgtg tccggcgctt
    22081 tcaacaagcc attgccccag cggcaaagcc ataacgctct gatacatgag ttcaattttc
    22141 cggccttctt cggcagtgat aaccgttttt cctgtgcttg cgcattccct cagaaacgct
    22201 ttctcttctt cttttccgga gtttgtccgg cggttaaatt caggtgctac gataaagcgg
    22261 ttactgaatt cttccggctc aagtacccgg cagtggaaag cggttcctaa atcgagcgtt
    22321 tttgtctttg tggtgtccac gggggcattt ttacgccaca aatacagtgc cggagtatca
    22381 gcaatgtcat cgagctgaga cttactgaca ccgggacccg cgtggtaatt ctcattcgaa
    22441 attccgtaat aaatacctgg ctctatgtct tctacgatta cgggatctgc gacttcgcca
    22501 gtttcatcac tgcaatcgcg atgcggatcg ctgccagcat tctcattgtg cggatgttca
    22561 gcgccttcca tttcctccgg atcattttcc ttagcttcaa cctgactctc ttcatcgaat
    22621 gtttccaggt atgttgcgtc gcccatcacc gcaccacagt cagggcagtt atccccgcca
    22681 gtctggccgc aggcattgca ggctatttcc ggttcctgtt gcactactgg ctcaggttga
    22741 ttcatatccg ggctggtttt ttccgtttct ggctggttct ggtacacaca atcgcgagtc
    22801 tggatcccct ttacccattt cggatcgttc gggtcgctaa ttccgtcaac aaattcacca
    22861 cgtgatgcag caagcaattt atcggcatcg acaggatttt ttgatggaat gtttttccgg
    22921 gcttcatgga gttctgcccg cagttcctga tatttcgcat caacagaatt tacctgtgac
    22981 tgagcatcca gcggctgcgt gtcctgatga tgttcagttg cgtccggttc cattgtttca
    23041 gcctctccct gttcaactgc cgttgttcca gatggttgcg gtttttcttc ataatcctgt
    23101 tttccttctt ctgttactcg ctgcggcatc ggggcagagg agcgaccgca ggcaatatcc
    23161 acgatttccg gatcagggtt ggcatgatcg gtttcagtca gtactttgtt cagatattca
    23221 gtgacgtgcg cggggatgac ctcgatccca attggtgctt cttttacgga cgcaaccacg
    23281 atggcgcggg aataatccag cccgccaggc atggtgatga atttgtcgcg gaaaacagaa
    23341 aagggcggtt tattttcagc gataatttcc tcaatgcgtt tagcgtgtgc cggatgaagg
    23401 ttatagatgt ccagatccat tgaacgggcc agtacgccag tggctacgtc ccgcgccagt
    23461 gacgtcagat cgtgtacgaa accttcgccg cgatcggtga ggtttccgcc gccagcatta
    23521 gcaccggaag ccgtgcgagt gatgtgtgaa acacgattac ccttcatcca ctcttttgtc
    23581 agcagtcctc gatcggtgta gtcagcgttc aggtatgctt cgaaaaaagc agttatcagt
    23641 cccaggtttg aattaccagg attagggaaa actttgtcag tgtcacgaac cagtttgtgg
    23701 agttcgcgaa tttccagcgg gtcgagcagg ctggttttgt gggaaacagc cagggcagta
    23761 acagccggta gttcttcagc ccgagcaatg tgtaatgcct ggagtccgtc gcgtgaaacg
    23821 tgcgttaccg gtttttcgct gccgtgttga gcaagccaac gaatgggcag ttcctggcca
    23881 gaaattggga gtagcatatt ctcctcaatc tcagtcatgt cttcgccgtt gacgttggta
    23941 ttgccttgat agtgagcgtt gtctggtgct gctcccggtt ttagttccca tgtcatggag
    24001 tctttgctga gttgatagcg ttcactccag gtaaaatcga tctcaccttc agcgggcagg
    24061 tcattaacga caggaaaatt cgtggcaaca gctttaaaat agctgctcag ttttttacct
    24121 gacttaacga tcaggtagtc cagagtggca caggtcgatt caaaatcgtt gcttgcccac
    24181 aggacgacgt caggttcacc ggatgatttt ttcgctttcc gtaacaggaa gagtggtttt
    24241 gtgctcattg ttttttaacc tcaactcaga ttaaaattcg ttttgttcag tgaatgatct
    24301 tgccggatac acactgttca tagcctgcgc catacgcagg ctatttcttt cagatttcac
    24361 cttttaattt cattgcaatt agagttgcca gaaattcggc ttttttttct gcgggcagat
    24421 tctttccgat atgcaccagg cacatttttt tgacaccttc atcaagtgtt tttacgttgc
    24481 ctgatggacc atcgatatca accacagtga atggggtttc tttattttct gttttaatta
    24541 cgtagccaat gcgctttcct tccagattca cctcgtgaac aatgtcatcg gtagttacaa
    24601 cagtggcttc ataattggta atcatgtttt tctccttaat taaggttgag cgaatacctg
    24661 ccatttctgg cataaattca gtttcgaata gtcaattaat taaagttcat gtgccatctg
    24721 gtctttttcg gcacaagctt cactgcaata ttttctcggt tcgtcttttg ataaaatccc
    24781 gtgcatgaag tgaagcattc tttcaatagc tttgctttct tcaacgtctt ttttgcaaag
    24841 gtggtaagca cattttattt tcttagtcat caccatgact ccgcctttac aggtaaacca
    24901 tcacgaccga ggaagacttt aatcatgcgg tcagtaatgc atgtttttgt ggtcaggtta
    24961 cgaatatata gttttcgctt tttaatattg tttgccgagg caatatatgt ccggccttca
    25021 tgaagaacat aatc
//
LOCUS       DAZGOB010000065        24671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGOB010000065 DAZGOB010000000
VERSION     DAZGOB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.065"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            304..1509
                     /gene="gltS"
                     /locus_tag="ACHLIF_002824"
     CDS             304..1509
                     /gene="gltS"
                     /locus_tag="ACHLIF_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO9606815.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            1512..2378
                     /locus_tag="ACHLIF_002825"
     CDS             1512..2378
                     /locus_tag="ACHLIF_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606816.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(2363..4444)
                     /gene="recG"
                     /locus_tag="ACHLIF_002826"
     CDS             complement(2363..4444)
                     /gene="recG"
                     /locus_tag="ACHLIF_002826"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO9606817.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(4450..5139)
                     /gene="trmH"
                     /locus_tag="ACHLIF_002827"
     CDS             complement(4450..5139)
                     /gene="trmH"
                     /locus_tag="ACHLIF_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO9606818.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(5146..7254)
                     /gene="spoT"
                     /locus_tag="ACHLIF_002828"
     CDS             complement(5146..7254)
                     /gene="spoT"
                     /locus_tag="ACHLIF_002828"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO9606819.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(7273..7548)
                     /gene="rpoZ"
                     /locus_tag="ACHLIF_002829"
     CDS             complement(7273..7548)
                     /gene="rpoZ"
                     /locus_tag="ACHLIF_002829"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO9606820.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(7603..8226)
                     /gene="gmk"
                     /locus_tag="ACHLIF_002830"
     CDS             complement(7603..8226)
                     /gene="gmk"
                     /locus_tag="ACHLIF_002830"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO9606821.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8484..10166
                     /gene="ligB"
                     /locus_tag="ACHLIF_002831"
     CDS             8484..10166
                     /gene="ligB"
                     /locus_tag="ACHLIF_002831"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO9606822.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(10163..10780)
                     /locus_tag="ACHLIF_002832"
     CDS             complement(10163..10780)
                     /locus_tag="ACHLIF_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO9606823.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11072..11896)
                     /gene="dinD"
                     /locus_tag="ACHLIF_002833"
     CDS             complement(11072..11896)
                     /gene="dinD"
                     /locus_tag="ACHLIF_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO9606824.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(12117..12980)
                     /locus_tag="ACHLIF_002834"
     CDS             complement(12117..12980)
                     /locus_tag="ACHLIF_002834"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO9606825.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            13107..13823
                     /gene="rph"
                     /locus_tag="ACHLIF_002835"
     CDS             13107..13823
                     /gene="rph"
                     /locus_tag="ACHLIF_002835"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO9606826.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            13889..14530
                     /gene="pyrE"
                     /locus_tag="ACHLIF_002836"
     CDS             13889..14530
                     /gene="pyrE"
                     /locus_tag="ACHLIF_002836"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO9606827.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(14567..15163)
                     /gene="slmA"
                     /locus_tag="ACHLIF_002837"
     CDS             complement(14567..15163)
                     /gene="slmA"
                     /locus_tag="ACHLIF_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO9606828.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(15270..15728)
                     /gene="dut"
                     /locus_tag="ACHLIF_002838"
     CDS             complement(15270..15728)
                     /gene="dut"
                     /locus_tag="ACHLIF_002838"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO9606829.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(15706..16926)
                     /gene="coaBC"
                     /locus_tag="ACHLIF_002839"
     CDS             complement(15706..16926)
                     /gene="coaBC"
                     /locus_tag="ACHLIF_002839"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO9606830.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            17098..17766
                     /gene="radC"
                     /locus_tag="ACHLIF_002840"
     CDS             17098..17766
                     /gene="radC"
                     /locus_tag="ACHLIF_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO9606831.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            17983..18219
                     /gene="rpmB"
                     /locus_tag="ACHLIF_002841"
     CDS             17983..18219
                     /gene="rpmB"
                     /locus_tag="ACHLIF_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO9606832.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            18240..18407
                     /gene="rpmG"
                     /locus_tag="ACHLIF_002842"
     CDS             18240..18407
                     /gene="rpmG"
                     /locus_tag="ACHLIF_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO9606833.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            18505..19314
                     /gene="mutM"
                     /locus_tag="ACHLIF_002843"
     CDS             18505..19314
                     /gene="mutM"
                     /locus_tag="ACHLIF_002843"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO9606834.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(19353..19832)
                     /gene="coaD"
                     /locus_tag="ACHLIF_002844"
     CDS             complement(19353..19832)
                     /gene="coaD"
                     /locus_tag="ACHLIF_002844"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO9606835.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(19840..21117)
                     /gene="waaA"
                     /locus_tag="ACHLIF_002845"
     CDS             complement(19840..21117)
                     /gene="waaA"
                     /locus_tag="ACHLIF_002845"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO9606836.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            21530..22588
                     /gene="rfaQ"
                     /locus_tag="ACHLIF_002846"
     CDS             21530..22588
                     /gene="rfaQ"
                     /locus_tag="ACHLIF_002846"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO9606837.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            22585..23709
                     /locus_tag="ACHLIF_002847"
     CDS             22585..23709
                     /locus_tag="ACHLIF_002847"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO9606838.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVCSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARYYADTQDLYSLPEKAADIITGGLDG"
     gene            23702..24499
                     /gene="rfaP"
                     /locus_tag="ACHLIF_002848"
     CDS             23702..24499
                     /gene="rfaP"
                     /locus_tag="ACHLIF_002848"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO9606839.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMHGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         5801 a         6080 c         6635 g         6155 t
ORIGIN      
        1 tgactcgagg gtggaaacag acatcgaaaa caatcccgtg gtagcaaagc gggcatttta
       61 gctgaccgaa agagaaaagc aaacgtttgc cacatcctca tctaaatatt taaatgagat
      121 aaatgcactt ttttatataa cttttggtat ttttctgctt aaaatccatg ccatttgttc
      181 attaacagct gagaatttga cccaggaaag cataactcga tacccgtcat agttcatgaa
      241 gttgccactg cgcgatgaag tatgacgagt atgaaagagt gatgcggata caaaggagta
      301 actatgtttc atctcgatac tttagcaacg cttgttgccg caacgctgac gttgctgctc
      361 gggcgtaagt tggtccattc cgtctccttt ttgaagaaat acaccatacc ggaacctgtt
      421 gcgggtggtt tgttggtggc gctggcgcta ctagtactga aaaaaagcat gggctgggaa
      481 gtcaactttg atatgtccct gcgcgatccg ttaatgctgg ctttcttcgc caccattggc
      541 ctgaacgcca acattgccag tttgcgtgcc ggtgggcgtg tggttggcat cttcttgatt
      601 gtggttgttg gtctgttggt gatgcaaaat gccattggca ttggtatggc tagcctgtta
      661 gggcttgatc cgctgatggg gctgttggcc ggttctatta cgctttccgg cggtcacggt
      721 acgggcgctg cgtggagtaa attgttcatt gaacgttatg gcttcaccaa tgcgacggaa
      781 gtggcgatgg cctgtgcaac gttcggtctg gtgctgggcg gcttgattgg cggtccggtg
      841 gcgcgctatc tggtgaaaca ctccaccacg ccgaacggta ttccggatga ccaggaagtc
      901 ccgacggcgt ttgaaaagcc ggatgtggga cgcatgatca cctcgctggt gctgattgaa
      961 actatcgcgc tgattgctat ctgcctgacg gtggggaaaa ttgttgcgca acttttggct
     1021 ggcactgctt ttgaactgcc gaccttcgtc tgtgtactgt ttgttggcgt gattctgagc
     1081 aacggtctgt caatgatggg cttttaccgc gtctttgagc gtgcggtatc cgtgctgggt
     1141 aacgtaagcc tgtcgttgtt cctggcgatg gcgctgatgg ggctgaaact gtgggagctg
     1201 gcttcgctgg cgctgccgat gctggcgatt ctggtggtac agaccatctt catggcgttg
     1261 tatgccatct tcgttacctg gcgcatgatg ggcaaaaact acgatgcggc agtgctggct
     1321 gcgggtcact gtggttttgg cctcggtgca acgccaacgg caatcgccaa catgcaggcg
     1381 atcactgaac gctttggccc gtcgcacatg gcgtttttgg tggtgccgat ggtcggtgcg
     1441 ttctttatcg atatcgtcaa tgcgctggtg attaagctgt atttaatgtt gccgattttt
     1501 gccggttaaa aatgaaaaag cgtcggaagg aaccggaaac gctacgtgaa cactgtcgcc
     1561 atatttttgg tgatgagccg ccagtgctct gtgtatggga aacggaattt gattacgcag
     1621 atgccgaact caaggcgctg gcggctaaag agtggcagca gatcagcgaa tgggatttga
     1681 gtgcttacta tgtactcaat ctcgtttata acgaaccgat gcaaatagag ttgtttcgct
     1741 atctgtttcc gctctgcctg gcgcaatggc atgaaaccgt tttagctggc ggttatggtg
     1801 atcacttcga agaatcctta atgaaggcat tgtgtcggcc ctatctctgg caggagatga
     1861 tgaatgcttc ccaacgccag caggtacggc aatttttact ggacaccgcg ttgcagcgta
     1921 tggataacga gcgtggtttc aataacgttc tttgctggct ggcggtgttc aatactctgg
     1981 gtggggccgc gccgttaatt cgttctctct ggtcgcgctg gtgggcgctg gatacgccag
     2041 gaaaagcggt gtgtgctatt cagtatgccg cgcatctaat ttatcccata gaagccaatc
     2101 cgttatggtc gcaagaatgg ataggctggg ggcatccatt aggccataaa gatggctggt
     2161 cctctgataa tcgcgctttt cttcgccaaa tgttaacacc tgaaatgatt gtggctggcg
     2221 tacaggcagc ggctgaaatt ctgcgtggtg aaccggaagg cgcaatggcg gcgcgaattg
     2281 cacaggatgc gtatgaggca atggatatcc tcactatcca gattgaagat ttactgcgtg
     2341 acttgtcttg tgatgagtct ggtcatgcgt tggagtaacg ttccgtctcc ggcatccagc
     2401 gttctatcag ggcttttgct tgttgtgggt aacgttcgtg aatatgtcgc gccaggcgct
     2461 gaacttccgg gatcatcgcc tgatcgcgca gtaaatccgc cactttaaac tcagcattac
     2521 ccgtctgacg cgtacctaac agttcacccg gtccgcgaat ctctaagtct ttctgtgcaa
     2581 tcacaaaacc gtcgttgctg tcgcgcagca cttgcagccg aatttgcgcg gttttggaga
     2641 gcggcgtttt gtagagcagc acgcagtgag aagccaccgc accacgacct acacgcccgc
     2701 gaagctggtg taactgcgcc agacccagac gctccgggtt ttcgataatc atcagactgg
     2761 cgttaggcac atcaacgccg acttcaataa cggttgtggc aaccagcagg tgtagctcac
     2821 cttgtttaaa cgacgccatc accgcctgtt tctcggcagg tttcatccgc ccgtgtacca
     2881 ggccaacgtt caactctggt agcgccagtt tcaactcttc ccaggtagct tccgccgcct
     2941 gcgcttccag caattccgac tcttcaatca acgtacaaac ccagtatgcc tgacgacctt
     3001 cagttatgca ggcgtggtgc acgcggtcaa tgatgtcggt acggcgggta tcaggaatag
     3061 cgaccgtagt cactggcgtg cggcctggcg gcagctcatc tatcaccgag gtatcgagat
     3121 cggcatacgc agtcattgcc agcgtgcggg ggatcggcgt ggcggtcatg atcaactgat
     3181 gcggatggaa gccctgctgc tggcctttct cccacaatgc cagacgctga tgcacgccaa
     3241 aacgatgctg ttcgtcgata atcaccagcg ccaggccgtt aaactgcacc tgttcctgga
     3301 agatggcgtg tgtaccgaca atcatctgca cctgaccgct ggcgatggct tcctgctgtg
     3361 ccagccgtgc tttacctttc tgcttaccgg cgagccagcc cacttcgata ccgagcggtg
     3421 caaaccagtt gcggaagtta ttggcgtgct gctcggcgag taattcggtt ggtgccatca
     3481 atgctacctg tttgccgtgg gcaatcgcac gcaacgcggc gagggcggcg accagcgttt
     3541 taccggaacc tacatcgccc tgcaccagac gcatcatcgg cacatccagc gccatatcgc
     3601 gctcgatctc cgccactacg cgtgcctgtg cgcccgttgg cttgaacggt aaggcggcga
     3661 ggagtttatt tttcagcgtg tcattggcgc tcagcggctg ggcatgaaaa cgctgtgctc
     3721 cggcacgtaa ggctaacatg ctgaggttgt gcgccagcag ttcttccaga atcagacgac
     3781 gttgcgccgg atgctgcccg gtttccagat cgctaagctg tagcgtcggt ggcgggcggt
     3841 gcaaagtgcg caacgcttcc ggtagcgtca tcattccttg tgacagttcc ggcggcagga
     3901 gttcttcaat ggcgcaggtg tcgagcagat ccagcgcctg gtcggttaat ttacgcagcg
     3961 tggcctgctt tacgccttcc gttgttggat aaaccggcgt gagcgtttcc tgtaattctg
     4021 gcgtgctgag atcgccctgc acgcggtatt ccgggtggat catctccgca ccatatttac
     4081 cgcgctttgc ttcgccataa gccagtacac ggcggcccgc cgccaggcta tttttcattg
     4141 ccgcgctgaa attgaaaaag cgcatggtga ggatgccgga accgtcgctg atctggcagg
     4201 tcatcatccg ccgaccgccg aaggagatat tgcagttcag cacttcgcct tccaccgtgg
     4261 cataaacgcc cggcagtagt tctccgatgg ggtagagatg ggtgcgatct tcgtagcgca
     4321 ggggaaggtg taagagtaga tcctgcacgg tatgcaggtt gattttcgcc agtttgttac
     4381 taagtgctgc gccaacgccc gttagggaac tgagtgggac agcatctaac aggcgacctt
     4441 tcatggcact taccctgcag cctgcatagt agcccaccag tcggcatcag cttcgatctc
     4501 gccttgctga ttgacgtggg gataaggcag gcctttgcgt tttgcgactt tcgccagcac
     4561 cggatagccg ccttcaaaca acaggcgttg ttgctctgct tccggcaaca tgctgttttc
     4621 acgcaggtac atgcctgcat tttgccgctg acgctgggct tcgtaaagaa tgagggctga
     4681 ggcaacggac acattcagcg actgcaccat gccgatcatc ggaatgatga tgtcctgatc
     4741 cgccagggcc aatgcttcct gcgtgatgcc cgttttctcc tgtcccatca aaatgcaggt
     4801 cgggcgagtg taatcaattt cgcggaaatc gacagcgtta tcagaaagat gggttgccag
     4861 aatctgcatg ccctggcctt tgagatgagc gacggcatcg ccaatggtgc ggtgtgtttt
     4921 cacctgtacc cagctgttac tacccgccgc tgccgaagcc atggtgcgca tgcggctacc
     4981 aggccagacg gcgtgaactt catgtacgcc aacggcatct gcggtacgaa taatcgcaga
     5041 aacgttatga ggtttgtgga cctgctccat gcagacggtc agatcaggct gccgcctggc
     5101 gagcatttcg cagatgcgtg cataacgtgt tgggttcata aaacattaat ttcggtttcg
     5161 ggtgacttta atcacgtctg gcatcacgcg gattttgcgc atgatattcg ccagatgcac
     5221 acggtcacga gcggtcagac gaataaaggc gctgtagacg cgaccatctt tctcttccgt
     5281 attcaaactt tgaatattcg aggtcgtggt gttaattgcc gccgtcaggt ttgccagcgc
     5341 accctgatga ttgaacatct ccaccttgat ttcggtgatg aactcctgcg ccgtctcttt
     5401 atcccattcc acagccataa acttctctgg ctctttctgg tagccacgga tgttacggca
     5461 ggattcatgg tggatcacca gacctttacc ggggctgacg tgggcgataa tcgggtcgcc
     5521 aggaatgggg cggcagcatt tcgcaaaggt gatcagcacg ccatcagcgc ctttgatcgg
     5581 cagatgcccg tggctttgag tcgccggtgg aatggaggcg tccccatgtt gtagattttt
     5641 cgcgaccacc acgctcattg cgttaccaag gccgatttct gccagcagat cgtcaagcgt
     5701 tgccagcttc atgcgatcca gctcgcgctg aatattttcc tgcgggattt cattgagctt
     5761 acggctacca cccaaagcat ggttgagcag acgacggccc aggcttacag aatcatcacg
     5821 cttgaggttt ttcagcaact gacgaatttt ggcgcgcgct ttcgagctaa cgacaaagtt
     5881 cagccaagcg gcattcgggc gagcgcccgg agcggtaatg atttcaacgg tctgaccgct
     5941 agtaagcggc tgcgacagcg ggtaaggctg gcggtcgacg cgtgcgccca cgcaggcatg
     6001 gccgatatcg gtatgcactg cataagcgaa gtcgacgggc gttgcaccgg caggcagctc
     6061 gacaatgcgc ccttccggtg tgaaaacgta aatctcatcc gggaagagat cggatttaac
     6121 gctctcgata aattcaaacg aactaccggc gctctgttgc agctccagca ggctttgcat
     6181 ccagcgctgg gcgcggattt gcgcggtggt gctggtttcg ccgtgctctt tataagccca
     6241 gtgcgcggca acacccatct ccgccatctg atccatatct tcggtacgga tctggacctc
     6301 aaccggcaca ccgtgcgggc cgatcatcga ggtgtgcaaa gactgatagc cgttcgcttt
     6361 tggaatggcg atgtagtctt tcacgcgacc cggacgcggc ttgtacaggc tatgcatttg
     6421 cccgagcacg cgataacagg tgtcggaatc attgacgatc acgcggaaag cgtagatgtc
     6481 catgattgag tgaaaacgct gctctttgag caccattttg cagtaaatcg aataaagatg
     6541 cttctcgcga ccactgacgc ggcacggaat tcccgcttcc tgcaaacgcc cttcgatttc
     6601 agagaggatt ttttggatca tctctttacg gttgccgcgc gcggctttca ccacttcttt
     6661 aattacgcgg taacgattgg gatacagcgc ctcaaaaccc agctcttcga gttcggtttt
     6721 aatgtggtgg atacctaaac ggtgcgccag cgggctgtaa atttcgagag tttcacgggc
     6781 gatgcggcga cgtttgtccg ggcgaagtga gcccagcgtg cgcatgttgt gggtacggtc
     6841 ggcaagtttg atgaggatga cgcggatatc ctgcaccatt gccataatca tcttgcgaaa
     6901 gttttcggcc tgcgcctctt tcttatcacg gaacttgagt ttatcaagtt tcgacacccc
     6961 ctctaccagc tcggcgacgc ttttaccaaa aagctgttcc atatcctggt aggtggcggg
     7021 agtatcttca atcacgtcat gcagcagcgc cgccatcagc gtttcatagt cgagtttcat
     7081 ctcggccaga atgcaggcaa ccgctaccgg gtgcgtgata tagggttcac cgcttgaacg
     7141 tgtttgcccc tcgtgagcat cacgtgcaac gagatacgcc tgccgcagac gcttgatttg
     7201 gtcttccggc aggtaggttt gaatcagttg attcaggctt tcaaacagat acaagggcga
     7261 cccgctttgt gattaacgac gaccttcagc aatagcggta acggcttgta attcagcggc
     7321 ttcctgctct tgctgttcct ggcgttcgcg aacgtcgagg atctggttgt tgatcagacc
     7381 ttcttcgatt tcgcgcagcg cgattacagt ggttttatcg ttttcttccg gtaccagcgg
     7441 atcctttccg cctacctgca tctgacgagc gcgacgcgcg gcgaccagta ccaggtcaaa
     7501 acggttacca attttctcta cagcgtcctg aacagttacg cgtgccatac ttaaaaagct
     7561 ccacaggtga agaaatgact gggcatgata ctgaaatcag gttcagtctg ccaacaattt
     7621 gctgattaaa gcgtcatgac gctgcttttg gcggctcatg cgcagacgtt cggcgcgaat
     7681 aatggtcttc aaatcggtca acgcggtatc gaagtcatca ttcacaatca gataatcata
     7741 ttcggcgtaa tggctcattt ctgcaacagc ttgcgccata cgctttgcaa tgacctcttc
     7801 gctgtcctga ccgcgaccgc gtagacggcg gtccagttca attttggacg gcggtaaaat
     7861 aaagatactc cgcgcgtgcg gcatcttctg gcgaatttgc tgcgcgccct gccagtcgat
     7921 atcgagaaaa acatcgacac cggtcgccag tacttgctca atggcctcac gcgaagtgcc
     7981 atagtaatta ccaaaaactt ctgcgtgttc gaggaacgca tctctgctaa tcatttcttt
     8041 aaattcatca tgattaacaa agaaataatg ttcaccgtgg acttcaccag gacgcggttg
     8101 gcgtgtggtg tgtgaaacag aaacctgggt gtcatacaac ggttgggttt ttaataaagc
     8161 ctgaatcagg ctggatttac ccgcgccact gggggcagaa acaatataaa gcgtgccttg
     8221 agccatgagt atctttcgta tgtgattagc gaaataaagc ctacatacga gcttattata
     8281 cacggcgcgg ccatgtgacg tagcccttgc tcacactttt tgcgcaaagt tgttgcgttt
     8341 tgcgtagctg tttccgcatt tgcctgtgat ttactgcaat aaagacgatt ctcaggaagc
     8401 gtcctcgcag actccgtttt tgccactcgg ctttgccttc accacgttgt atatctgcca
     8461 gcctgactac agcggaggtg aagatgaaag tatggatggc gatattaata agtatcttgt
     8521 gctggcaatc atctgtgtgg gcggtctgtc cggcctggtc gccagccaga gcacaggaag
     8581 aaatttcccg cctgcaacag caaataaaac agtgggacga tgactactgg aaggaaggaa
     8641 aaagtgaggt ggaagacggt gtttacgatc agttaagcgc ccgtcttacg cagtggcaac
     8701 gctgctttgt gagcgagccc cgcgacgtga tgatgccgcc tttaaatggt gcggttatgc
     8761 atccggttgc ccatacgggt gtacgcaaaa tggcggataa aaacgcatta agtctgtgga
     8821 tgcgagaacg tagcgatctt tgggtgcagc caaaagttga tggcgttgcg gtaaccctgg
     8881 tttatcggga cgggaaactg aacaaagcaa tcagtcgcgg taatggcctg aaaggcgagg
     8941 actggacgca gaaagttagc ttaatttccg ctgtgccgca aaccgttagc gggcctttag
     9001 ccaacagtac gcttcagggg gaaatatttc tccagcgcga ggggcatatc caacaacaaa
     9061 tggggggaat aaatgcccgc gcaaaagttg ctggcctgat gatgcgccag gacgatagcg
     9121 acacgctgaa ttctctgggc gtttttgtct gggcatggcc ggatggaccg cagttaatga
     9181 ccgatcgttt aaaggagctg gctaccgcag gttttactct aacgcagagg tatacccgtg
     9241 cggttaaaaa tgctgatgag gttgcgcgcg tacgcaatga gtggtggaaa gcgaaattac
     9301 ccttcgtcac cgatggcgtg gttgtgcgag gggcgaaaga acccgaatcc cgccattggc
     9361 taccaggcca ggcagaatgg ctggtggcct ggaaatatca acctgtagct caggttgccg
     9421 aagtgaaggc gattcagttt gcggtgggta agagcggtaa aatatcggtg gttgcgtcac
     9481 tcgcacctgt catgctggat gataaaaaag tccagcgggt gaatattggt tccgtcaggc
     9541 gctggcagga gtgggatatt gcgcctggtg atcagatcct cgtcagcctt gccggtcagg
     9601 gtattcctcg cattgatgat gtggtctggc gcggtgcaga acgtacaaaa ccgacaccac
     9661 cagaaaaccg ctttaactcg ttgacctgct actttgcttc tgatgtttgt caggaacagt
     9721 tcatttcacg cttagtctgg ctggggtcaa aacaggttct ggggctggat ggtattggtg
     9781 aggccggctg gcgcgcgctg catcagactc atcgctttga gcatatcttt tcctggcttt
     9841 tattaacgcc agagcaatta cagaacacgc cggggatcgc gaaaagtaaa agtgcgcagc
     9901 tatggcatcg gtttaatctg gctcgtaagc agccttttac tcgctgggtg atggcaatgg
     9961 gaataccgct aacccgggcg gcgcttaatg ccagtgatga acggtcctgg tcgcaacttt
    10021 tatttagcac ggagcagttc tggcagcagc tgccggggac tggatcgggg cgcgccagac
    10081 aggttattga atggaaggaa aatgcgcaaa tcaagaagct gggcagttgg ctggctgccc
    10141 agcagatcac aggttttgaa ccttagtgtc cttcgtggct gtagtaaaag accggtaatc
    10201 ccagcttaag gcgtagcgcc agtaagcggg caaaaaagcc gaataccagg gtggagataa
    10261 ttaccacatc atggttagaa acatagtgtt gcagcgcgat gtacagcacg gcggaggcaa
    10321 atgagacacc ggcatacaac tctttctgga ataccagtgg gatacgttta cagaacatat
    10381 cgcgcaaaac gccgccaaac acgccggtgg ttaccgccgc gacaacggca ataattgggc
    10441 cgtgccccat atccagtgca acctgtgcgc cgatgataga aaagaccacc agtccaagcg
    10501 catcgagcac cagaaacact ttgcgcaggt aaggcattac aggagcaacg atagtggtga
    10561 gtactgcggc ggtagcgacg ataatcacat attccgggtg tttgacccag ccgagcggat
    10621 agtggcccag cagaatatcg cgcactgacc ctccgccaat tgcggtggcg gtcgcaataa
    10681 taattacgcc aaatgtatcc atgcggcgtc gcccggccgc cagagccccc gtcatggctt
    10741 cggcagtaat gccaaccaaa tacaaaatgt gtaacagcat ggttctctcc ctaaaaatct
    10801 gtctggcagg gtagcgattt gtgcgtattg tcacgattga gattttttaa ggtgagagga
    10861 aattgataac ttgtagttat gtgaaatcgc tattttctgt agcgtagatg cattcttctg
    10921 acttcttgtc tgagaaaatg cataagaaaa aataggttaa aattgagggg tggaaaagaa
    10981 aggtgcaatg tttagccatt gtgttcaccg cacctggact ttggcttacg catcgggtat
    11041 ctcaatcaac aaaaagagtt tttagtttct gctatttctt ctctgtaatt tttacactgt
    11101 tttccaactg cttgatgctg acctgcgggg tcggcaactc ctcaggcatg gttccgccaa
    11161 gttcctgaat ggtttgcctc accttgctac ccacgtcaaa gtgtgtggta ttagcttgct
    11221 gttttgaatt cacgccatcc cgcttgagtt tttcttctgt ttgggtagct cgaaagagat
    11281 tagccgccag ttctgttgaa cccatatgat ccaggatctt ctgactcttt ttcagcccct
    11341 tccgctgatg gatggctttc tgatctaatc caccatacag cccccggtaa ccatgattct
    11401 gaaatatggc gaaatcgata gctgtcgcta ccccagcttg ctgcgctgcc tcaaccaatt
    11461 gtttgttatg ttctttcaat tcatttctca gaaaaagacg cttttcgtct tcacgaagtt
    11521 gcctgaaagc ttcatcgtca gccagctcct gccgtcgggt ctggatagca aaataagttt
    11581 gccctgccgc aataaccggt ttcgcagggt cgccgttttg tactaccagg taacaggcat
    11641 agcgggagag atgaacatct tcaagttctc gttgagcccc tgaacctaat acaaccattt
    11701 tggtggtttc aacgaaatgg tcagatgcag cttgattgct ggcttcgcac gcttgagtcg
    11761 cgcgggccag taccttttga aaattgcgcc agtcccgata atccagtaac ggtgccaatt
    11821 ctctagctga ccaaaattct tgcccttcag tgccataatg ccgtatttct tcaaaaggtt
    11881 gatgatgttc gttcattgcc cactccatgt gatatcgatt gcaaagaaag taaatctgta
    11941 actgtattta tatacagttg tgagtatctc acgttctcag tgtgctgggc aaaaaaacat
    12001 cgcaaaatct ggagcaggca cgcaaaacag aattgaagtt gcggggaaga aaaatgccgc
    12061 tctgtttgag tgggcggcat ttgtgaaggc tgaaaagtaa gaattagtgt tacgagttac
    12121 tcaatattct gaatctgctc gcgcatctgc tcaatcaaca ctttcagctc aatcgcggag
    12181 tttgtcactt cggcattgat agacttcgac gcaagagtgt tcgactcgcg gttgaattcc
    12241 tgcatcataa agtccagacg acgaccaacg gcttcttttt tcttcagaat gttgtaggtc
    12301 tctttgacat gcgcttccag gcggtccagt tcttcggcaa cgtcaattcg ttgtgccagc
    12361 agaaccaact cctgctccag gcggttgttt tccagttgca cttgagcatc ttccagcttc
    12421 gcgaccagac gctcacgctg ccattgcagg atttccggca tatgggcgcg gactttgacc
    12481 acttcggcgg tgacgccttc cagacgctgc tcgatcaatg ctttcagcgc ctgaccttcg
    12541 gtttcacgtg cgacgataaa gtcgtccagg gtaccatcca acgccgcgag aatttcagcg
    12601 gcaatcgcgt cgagatcctg ctcctgggct gccattacgc ccggccagcg cagaatatca
    12661 accgggttga tttccccttc gtcactctgc atttttaccc agttcgcggc agttaccagc
    12721 tgtttagcca gtttttcgtt gaggatcagc tcaccttgcg cgctaacatc tggctcatag
    12781 cgcagggtac attccacttt accgcgcgtc aggcgagaac gaatacgctc gcgaacgaca
    12841 ggttcaaggc tacggaactg ctccggcaga cgaaagtaag tttccagata acgctggttt
    12901 accgagcgca tttcccaggt tgcgctcccc cattcaccct tgatttcacg ccgggcgtag
    12961 gcggtcatac tgcggatcat agacgttcct gtttataaaa ggagaggtgg aaggattata
    13021 gccatcgatg ccttgtcagg ataggaataa ccgccggaag tccgtataat gcgcagccac
    13081 atttgtttca agccggagat ttcaatatgc gtccagcagg ccgtagcaat aatcaggtgc
    13141 gtcccgttac cctgactcgt aactatacaa aacatgcaga aggctcggtg ctggtcgaat
    13201 ttggcgatac caaagtgttg tgtaccgcct ctattgaaga aggcgtgccg cgcttcctga
    13261 aaggtcaggg ccagggctgg atcaccgcag agtacggcat gctgccacgt tctacccaca
    13321 cccgtaacgc tcgtgaagcg gcgaaaggta agcagggtgg acgcacaatg gaaatccagc
    13381 gtctgatcgc ccgtgctctt cgcgcggcag tagatttgaa agcgctgggt gagttcacca
    13441 ttacgctgga ctgcgacgtg cttcaggctg atggtggcac gcgtaccgcg tcgattacgg
    13501 gtgcctgcgt ggcgctggca gatgcgctac agaagctggt ggaaaacggc aagctgaaaa
    13561 ccaacccgat gaaagggatg gtagccgcag tttctgtcgg tattgtaaac ggtgaagcaa
    13621 tttgcgatct ggaatacgtt gaagactcag ccgcagagac cgacatgaac gtagtgatga
    13681 ccgaagacgg gcgcatcatt gaagtgcagg ggacggcaga aggcgagccg ttcacccatg
    13741 aagagctact caccttgttg gcgctggccc gagggggaat cgaatccatt gtagcgacgc
    13801 agaaggcggc gctggcaaac tgatttttaa ggcgactgat gagtcgcctt ttttttgtct
    13861 gtagaaaagt aagatgagga gcgaaggcat gaaaccatat cagcgccagt ttattgaatt
    13921 tgcgcttggc aagcaggtgt taaagtttgg cgagtttacg ttgaagtccg ggcgcaaaag
    13981 cccctatttc ttcaacgccg ggctgtttaa taccgggcgc gatctggcac tgttaggccg
    14041 tttttacgct gaagcgttgg tggattccgg ccttgagttc gatctgctgt ttggccctgc
    14101 ttacaaaggg atcccgattg ccaccactac cgccgtggcg ctggcggagc atcatgacct
    14161 tgacctgccg tactgcttta accgcaaaga ggcaaaagac cacggtgaag gcggcaatct
    14221 ggttggtagc gcgttacaag gacgcgtaat gctggtagat gatgtgatca ccgccggaac
    14281 ggcgattcgc gaatcgatgg agattattca ggctaatggc gcgacgcttg ctggcgtgtt
    14341 gatttcgctt gatcgtcagg aacgggggcg cggcgagatt tcggcgattc aggaagttga
    14401 gcgtgattac aactgcaaag tgatctctat catcaccctg aaagacctga ttgcttacct
    14461 ggaagagaag ccggaaatgg cggaacatct ggcggcggtt aaggcctatc gcgaagagtt
    14521 tggcgtttaa agaaactcgc cggatgaaaa gtcatccggc gtcatattac tgcaactgtg
    14581 ccgcaattag cggccagcgg gcgtcaaaat catccgtcgg gcggtattta aattcgctgc
    14641 ggacaaaacg tgacagcata ccttcacaga aggccaggat ctggcttgcc agcagggttt
    14701 catcggtggt gtaaccttca ccctcacgca ttctcttttc acgcaatacc tggcgcagct
    14761 gcgcttcaat acgctcgaac agctggttga tgcgcccttg caggcgatcc tgttcaaaca
    14821 ttagcgcatg accagtgagg atgcgggtca ggccaggatt acgctcacca aaaccgagaa
    14881 gcagcaacac aatcagacgc aggcgcgctg tggtgtcttt ctcatctttc agaatcaggt
    14941 tgatgcgagt aatcaggcta tcttcgataa actcaatcag gctatcgaac atgcgggtct
    15001 tactggggaa gtggcgatac agtgccgctt cggaaacgcc gacagaggcg gccagttttg
    15061 ccgtcgtgat acgttggctt ccatcgctgg attccagcat cagcgccaga gactgaagta
    15121 tttcctcgcg acggttcctt ttcgcagttt gtttttctgc catgttacaa aatacccctg
    15181 aaaataagca cttgccaggc ggcacccacg ctatgaccgc aaacgaaatg tttgcggcta
    15241 tgttatgacg ttattcggat gcgtatgtgt tactgacgac cagagtgacc aaagccgcct
    15301 tcaccgcggt cggtggcgtc gaaatcttcc accagattaa attcagcctg tactaccgga
    15361 acaaaaatca tctgggcgat gcgttcgcca ggttgaatgg tgaagctgtc ctgaccacgg
    15421 ttccacacgg aaatcatcaa ctggccctga tagtcagaat cgatcaatcc taccaggtta
    15481 ccaagcacga taccgtgctt atgtcccaat ccggagcgcg gcagcatcat tgccgccagt
    15541 gaaggatcgg caatatgaat cgccagcccg gtcggaacca gcgtagtgtc acccggagcc
    15601 agttctacgg cgtcgtcgag acaggcacgc aggtcaagtc cggcagagcc agaggtggca
    15661 taagtcggga gcggaaattc cttcccaacg cgcgggtcca gaatcttaac gtcgattttt
    15721 ttcatcataa cgggtcacga tctcgtcgag taataattgg ccaaggagct ctttgcgctc
    15781 aagcggtaag actttatctc cgtcctgcca gaaaaggtgt aatgcgttgt tgtcgctgtt
    15841 aaatccttga gttggctggg aaacatcgtt cgcgcagatc agatcaaggt ttttacggat
    15901 acgtttttgc cgggcgtatt cttccacatt atttgtttcg gcggcaaatc caacgacgta
    15961 gggtcgatgg tcttttagtg cggcaacgcc tgcgacgata tcggggtttt taaccatttt
    16021 tattgttaat tcatcaccct gcgtggcctg ctttttgatt ttctctgggg ccacggtagc
    16081 tgcgcgataa tccgccacgg cggcgcagcc gataaaaata ttttgctgct gtacagaagc
    16141 attcacggcg gcttccattt ccagcgcagt catcacatca acacgtttaa caaacggtgg
    16201 cgtcggtagt gaaaccggac ctgataccag cgtgacgttc gcgccacgac gggcagcggc
    16261 ggcggcgata gcaaaaccca tcttgccgga gctgtgatta gagatataac gcaccggatc
    16321 gagcggttca cgcgtcggac cggcggtaat cataatgttc agatgtttca ggtcgttgac
    16381 gggcgaaaaa tgcgccaccg ccatatccac aatggttaac ggatcgagca ttcgcccagg
    16441 accgatatca ccacaagcct gactgccact gtctggcccc cagatgagca aaccacggga
    16501 agcaagcacc tctaaattat gctgcgtggc agcggcacgg tacatctgct ggttcatggc
    16561 ggggagcacg gctacaggcg caggtgtagc cagacaaatc gtcgatacca ggtcattcgc
    16621 cattccggca gcaacacggg caatcaaatc tgccgtggca ggggcgagaa tcactaaatc
    16681 agcccattta cccagctcaa tatggcccat agcggcttcg gctgccgggt ccagcaaact
    16741 gtcggaaacg ggataaccag aaaccgcctg caagctaagt ggggtgataa aggcttttgc
    16801 cgcttcggtc atggctacgc ggacgtcggc cccgcgatcg cgcaaacgac gcaccagttc
    16861 aggggtttta taggcagcaa taccgccgct aacgccgaga acgatttttt taccggccag
    16921 gctcatgatg atttttcctg ttgggtgaca ccagaagttg gcgattttat cacaatactt
    16981 ttgttgtcgt gccttcacct gagaatcact ttgcgaggcg ctttccagga ttgaaaactg
    17041 gccgtcgatt taacggaacg gctatgacag gatgcgagca ccacaaagga ggtgaaggtg
    17101 aaaaacaatg cacagctgtt gatgccgcgc gaaaaaatgc tgaagtttgg tattagcgcc
    17161 ttaacggatg tcgagctgct ggcgttattt ctgcgtaccg gaacgcgcgg taaagatgta
    17221 ttaaccctgg caaaagagat gctggagaat ttcggctctc tttatggctt gttaacctct
    17281 gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc ccagttaaag
    17341 gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga aagcccttta
    17401 ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga ggagcgggag
    17461 atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag ccgtcttttt
    17521 tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccgcga agcgataaaa
    17581 ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc tgaacccagt
    17641 aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat ggatttacgc
    17701 gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga acgcggctgg
    17761 atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt gagcacatcg
    17821 ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt gggcctggca
    17881 atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag gcgccaatac
    17941 cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg agtctgccaa
    18001 gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa cgcgactaaa
    18061 cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga gaagcgtttt
    18121 gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg catcgataca
    18181 gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg aaataaatca
    18241 tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact ggtcacttct
    18301 ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa aaattcgatc
    18361 cagttgttcg tcagcacgtg atctacaaag aagcgaaaat caaataattc tcgctttgat
    18421 gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc atattgattg
    18481 catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac cagccgtcgc
    18541 ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cagtggtgcg caacggacgc
    18601 ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt gcttagcgtg
    18661 cagcggcggg ctaaatattt gctgctggag ctgcctgagg gctggattat cattcatttg
    18721 gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa gcatgaccat
    18781 gtggatttgg tgatgagcaa cggtaaagtg ctgcgctaca ccgatccgcg ccgctttggt
    18841 gcctggctgt ggaccaaaga gctggaaggg cataatgtgc tggcccatct tggaccggag
    18901 ccgctcagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa gaaaaaaacg
    18961 gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa tatctatgcc
    19021 agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc actgtcgctg
    19081 gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc gattgagcag
    19141 ggcggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta tttcgcacag
    19201 gaattgcagg tttacgggcg aaaaggcgag ccgtgtcggg tgtgcggtac gccgattgtg
    19261 gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa gtaattcatg
    19321 cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccat cagcgcctga
    19381 tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac ctctttcacc
    19441 aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact ttccagttcc
    19501 ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc cgccaccgca
    19561 cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc catcaaatca
    19621 ctaaacccga ccacttccac gttccccaga tgcgcggttg cctgctgtgc cagcgccaca
    19681 cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc cagaataacg
    19741 tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt aatgggatcg
    19801 aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt tcggtggcag
    19861 gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat acagtacttc
    19921 aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag aggacacctc
    19981 ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct ccagccgcgc
    20041 gcaaatgtct ttaaagttaa aagtatgcgg ccccatcaat accggaatag cgtgtgcggc
    20101 agcttccagc ggattatgcc caccacgttc aaccagtgaa ccgccaacaa aggcgagatc
    20161 ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa cctgcgtgct
    20221 ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct ggcggacaag
    20281 attaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca ataaattcgg
    20341 gaattgctgt aacaatgcct gatgtgcggc gatcaccaca ctctcttcgc cttcgtgagt
    20401 gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg tcacggcttt
    20461 agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa cggtcacctg
    20521 attatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt gcgcagcaat
    20581 cagcgtaata cgacgcagca agcgacggac gaatttaccc agtttggcat aacctgcggc
    20641 cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt tgtgtagcgc
    20701 cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg ggtcgacttt
    20761 attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt gctgaacatc
    20821 cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg taacggtaat
    20881 cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg ccagagtttc
    20941 accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc ggtaaaaacc
    21001 gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca cccagagccg
    21061 tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt cgagcatagt
    21121 aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc tttaccatta
    21181 cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga acatctatac
    21241 tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa ggaatcgcag
    21301 agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct ttattgaaaa
    21361 tcaacaatgt gcttaaaaat cattcgggtc ggattgccgc gaaagaagtg atacactagc
    21421 gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg cggtagcgtg
    21481 ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg tggataagcc
    21541 atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat taactactcc
    21601 cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc tgctttatca
    21661 ggacaccatc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg gcataaaaaa
    21721 taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca aggtattacg
    21781 tgccaataag tatgacctta tcgtcaatct caccgatcaa tggatggttg ctatactggt
    21841 tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc agtctgcttt
    21901 ttggcgtaaa agtttcaccc atttggtgcc gttgcagggt ggaaatgtgg tggaaagtaa
    21961 cttatccgtg ctgaccccat tgggagttga ttcgttggtg aagcagacaa ccatgagtta
    22021 cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg ttggacaaaa
    22081 ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca acgccaagtt
    22141 ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga cgtccggccc
    22201 agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga cgccaccagt
    22261 aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg atcatgcgca
    22321 gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta atacgccgct
    22381 gatatcgctg tttggtgcga cagatcatat tttctggcgt ccctggtcaa ataacatgat
    22441 tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg accgaaatga
    22501 gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata aattactgcc
    22561 ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa atattttccc
    22621 tttggcggtt tgcagcgtga ttttatgcgt attgctcaga cagtcgccgc ccgaggtcat
    22681 catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt tgaactgatc
    22741 aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt tgcctgggtg
    22801 caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa aatgccgggg
    22861 ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtagcgca ggaaaaaggc
    22921 tttttctatc gcctgacgtc acgttatcgc cattatgccg cctttgagcg ggcaaccttc
    22981 gaacagggta agccgacaca gctgctgatg ctgacagata agcaaatcgc cgatttccag
    23041 aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat ttatcctgat
    23101 cgtaaatata gccagcagcc agccaatagc cgtgaaatct tccgtaagaa gaatggaata
    23161 accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa aggtgtcgat
    23221 cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac attgctatat
    23281 gttgttgggc aggataaacc gcgaaaattt gaggcgctgg cagaaaaacg cggcgtgtgc
    23341 agtaatgttc actttttctc ggggcgcaat gatgtctcgg aattaatggc ggcggcggat
    23401 ttattactgc atcctgccta ccaggaagcg gcgggaattg tgctgctgga agcgattact
    23461 gcaggattac cggtactaac aacagccgtt tgtggctatg cgccttatat tgtcgaggct
    23521 aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga gattttacgc
    23581 aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg atattatgct
    23641 gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac gggtggtctg
    23701 gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggcaaagatc cttttgagga
    23761 agttaaaacc ttgcagggtg aggtatttcg tgaactggaa actcgccgta ctctgcgctt
    23821 tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc tgaaagagat
    23881 aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg aatggaatgc
    23941 gattcatcga ctgcgggatg tcggcgttga tactatgcat ggggtggcat ttggcgaaaa
    24001 aggcatgaat ccgctgacca gaacttcatt tattattacc gaagatctga caccaaccat
    24061 aagtctggaa gattactgtg ctgactgggc gactaaccct ccagatgttc gcgtaaagcg
    24121 tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgcgg gcattaacca
    24181 ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta aggaagagga
    24241 gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg ttccacgtcg
    24301 ttggcgggat aaagatctta ttgggcttta tttttcttcg atgaatatcg gcctgactca
    24361 gcgggatatc tggcggttta tgaaagtgta ttttgccgcc ccgcttaaag acattctcaa
    24421 gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca gggaaagaac
    24481 gattcgaaaa tcgttgtaac ccgtaactta tttttgccaa aattttggat acagaataaa
    24541 tatgtctcaa ctcaatgata gtgacatcat cctttttgag tataattttc attatcaaaa
    24601 tataagatct aaaaatactc ttgatatagc atttggtatt gacagaaatt ttctttttgg
    24661 atgtggtgta g
//
LOCUS       DAZGOB010000066        24111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGOB010000066 DAZGOB010000000
VERSION     DAZGOB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.066"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..149
                     /locus_tag="ACHLIF_002849"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACHLIF_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005163699.1"
                     /note="catalyzes the formation of glutamate from
                     glutamine; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="ACHLIF_002850"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="ACHLIF_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO9606840.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /gene="digH"
                     /locus_tag="ACHLIF_002851"
     CDS             1828..3147
                     /gene="digH"
                     /locus_tag="ACHLIF_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO9606841.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLPQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVQELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3524..4906
                     /gene="dosC"
                     /locus_tag="ACHLIF_002852"
     CDS             3524..4906
                     /gene="dosC"
                     /locus_tag="ACHLIF_002852"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO9606842.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4931..7330
                     /gene="dosP"
                     /locus_tag="ACHLIF_002853"
                     /gene_synonym="pdeO"
     CDS             4931..7330
                     /gene="dosP"
                     /locus_tag="ACHLIF_002853"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO9606843.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7588..8169
                     /gene="ddpX"
                     /locus_tag="ACHLIF_002854"
     CDS             7588..8169
                     /gene="ddpX"
                     /locus_tag="ACHLIF_002854"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO9606844.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8183..9733
                     /gene="ddpA"
                     /locus_tag="ACHLIF_002855"
     CDS             8183..9733
                     /gene="ddpA"
                     /locus_tag="ACHLIF_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9606845.1"
                     /translation="MKRSISFRPTLLALVLATNFPVSHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9735..10757
                     /gene="ddpB"
                     /locus_tag="ACHLIF_002856"
     CDS             9735..10757
                     /gene="ddpB"
                     /locus_tag="ACHLIF_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9606846.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10754..11650
                     /gene="ddpC"
                     /locus_tag="ACHLIF_002857"
     CDS             10754..11650
                     /gene="ddpC"
                     /locus_tag="ACHLIF_002857"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO9606847.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LALAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11647..12633
                     /gene="ddpD"
                     /locus_tag="ACHLIF_002858"
     CDS             11647..12633
                     /gene="ddpD"
                     /locus_tag="ACHLIF_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9606848.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12626..13552
                     /gene="ddpF"
                     /locus_tag="ACHLIF_002859"
     CDS             12626..13552
                     /gene="ddpF"
                     /locus_tag="ACHLIF_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO9606849.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTVPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(13608..14039)
                     /gene="osmC"
                     /locus_tag="ACHLIF_002860"
     CDS             complement(13608..14039)
                     /gene="osmC"
                     /locus_tag="ACHLIF_002860"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO9606850.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14385..14600
                     /gene="bdm"
                     /locus_tag="ACHLIF_002861"
     CDS             14385..14600
                     /gene="bdm"
                     /locus_tag="ACHLIF_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO9606851.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            14702..14839
                     /gene="sra"
                     /locus_tag="ACHLIF_002862"
     CDS             14702..14839
                     /gene="sra"
                     /locus_tag="ACHLIF_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO9606852.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            14996..16693
                     /gene="maeA"
                     /locus_tag="ACHLIF_002863"
     CDS             14996..16693
                     /gene="maeA"
                     /locus_tag="ACHLIF_002863"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO9606853.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            16827..17837
                     /gene="adhP"
                     /locus_tag="ACHLIF_002864"
     CDS             16827..17837
                     /gene="adhP"
                     /locus_tag="ACHLIF_002864"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO9606854.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            17984..18268
                     /gene="yddM"
                     /locus_tag="ACHLIF_002865"
     CDS             17984..18268
                     /gene="yddM"
                     /locus_tag="ACHLIF_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9606855.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18319..18972)
                     /gene="fdnI"
                     /locus_tag="ACHLIF_002866"
     CDS             complement(18319..18972)
                     /gene="fdnI"
                     /locus_tag="ACHLIF_002866"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO9606856.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(18965..19849)
                     /gene="fdnH"
                     /locus_tag="ACHLIF_002867"
     CDS             complement(18965..19849)
                     /gene="fdnH"
                     /locus_tag="ACHLIF_002867"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO9606857.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(19862..22909)
                     /gene="fdnG"
                     /locus_tag="ACHLIF_002868"
     CDS             complement(19862..22909)
                     /gene="fdnG"
                     /locus_tag="ACHLIF_002868"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22322..22324),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO9606858.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23141..24022
                     /gene="yddG"
                     /locus_tag="ACHLIF_002869"
     CDS             23141..24022
                     /gene="yddG"
                     /locus_tag="ACHLIF_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO9606859.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5909 a         6109 c         6091 g         6002 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg acaatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgcta aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaacccac gtcaatgaaa
      841 tgagtaaccc agggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc tctggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taagtcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact agttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcggctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg cctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgcgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc cgtagatggc
     2521 gtgcagtttg acgactattt ctatacggag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggtgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac aggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccac agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccattctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttacc ctcttgaact atcccactac taatcatgct tactaaagtc
     3241 aaattaacca cgcaattaac cacacttagt taaacagcca taaatcccct aataaaaatg
     3301 tatgaatatg aaaaaacata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattaa aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgccgttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattct gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgctatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactaga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa atcctgctgg attctgattt
     4141 aggcagtagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agtggcattg ccgaagtagg ccatatctcc cgtctgattc aggatttcga
     4261 cggtatattc aatcaaacca tgcgtaacac caggaatttg aataacagaa gtctgcgggt
     4321 gaaattttta ttacagataa gaaatactgt atcgcaaatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 actgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctctcaggcc ttttatgaca acgtccgcag
     4621 tagtgattat gtttttcgct acgggggcga tgaatttatc attgttttga ctgaagcttc
     4681 tgaaaacgaa acgttacgta ccgcagaacg tattcgcagt cgggtggaga aaaccaaact
     4741 gaaagccgca aacggcgagg atattgccct ctcactttcc atcggtgccg ccatgtttaa
     4801 tggtcatcct gactatgagc gcctcattca aatagccgat gaagctctgt atatcgccaa
     4861 aagacgaggt agaaaccgtg ttgaactctg gaaagccagt ctttagatgc gccaggatgc
     4921 agaggtaatc atgaagctaa ccgatgcgga taatgccgcc gatggcattt ttttccccgc
     4981 ccttgagcaa aatatgatgg gtgcggtgtt aattaacgaa aatgatgaag tgatgttttt
     5041 caaccccgcc gcagagaagc tctgggggta caaacgtgaa gaagtcattg gcaataacat
     5101 tgatatgctg attccgcggg atttgcgtcc tgcgcatcct gaatacattc gtcataaccg
     5161 tgaaggcggt aaagcgcgtg tcgaggggat gagtcgggag ctgcagctgg agaaaaaaga
     5221 cggcagtaaa atctggaccc gttttgcgct atcgaaagtg agcgccgagg ggaaagttta
     5281 ttacctggcg ctggtacggg atgccagcgt agaaatggcg caaaaagaac agactcgaca
     5341 attgattatt gccgttgacc atctcgaccg accggtgatt gtcctcgatc cggaacgcca
     5401 tattgtgcag tgcaatcgcg catttaccga aatgtttggt tactgcatta gcgaagccag
     5461 cggtatgcag cccgatacac tcctgaacat tcctgaattc cctgccgata accgcattcg
     5521 tttacaacag ttgctatgga aaaccgcccg cgatcaggac gaatttctgc tgttgacgcg
     5581 caccggtgaa aaaatctgga ttaaagcctc tatcagcccg gtttatgacg tgctcgcgca
     5641 tctgcagaac ctggtaatga ctttctcgga tatcaccgaa gaacggcaaa tccgccagct
     5701 tgaaggcaat attctcgccg ccatgtgcag cagcccgcca tttcatgaaa tgggggaaat
     5761 catttgtcgt aacatcgaat ctgttctcaa cgaatcgcat gtttcgctgt tcgcactgcg
     5821 caacgggatg ccgatacact gggcgtcatc ttcgcacggt gcagaagttc aaaatgcgca
     5881 aagctggtca gcgaccattc gtcagcgtga tggcgcgcct gcggggatcc tgcaaattaa
     5941 aacctcgtca ggagcagaaa ccagcgcctt tatcgaacgc gtggcagata tcagtcagca
     6001 tatggccgcg ctggcgctgg aacaggaaaa aagccgccag catattgaac aactcatcca
     6061 atttgatccg atgaccggtc tgccaaatcg caataacctg cacaattacc tcgatgacct
     6121 ggtcgacaaa gccgtctctc cggtggtgta tctcatcggt gttgaccata ttcaggatgt
     6181 gattgatagc cttggctatg cgtgggccga tcaggcattg ctggaagtgg tcaatcgctt
     6241 tcgtgaaaaa ctcaaaccgg atcagtatct ctgtcgtatc gaaggtacgc agtttgtcct
     6301 cgtgagcctg gaaaacgacg tcagtaacat tacccagatc gccgatgagc tacggaatgt
     6361 ggtcagcaag ccgataatga ttgacgataa accattcccg cttaccttga gtattggcat
     6421 cagctacgac gtgggtaaaa accgcgatta cctactctcc actgctcaca atgcaatgga
     6481 ttatattcgc aagaatggcg gtaacggctg gcagttcttc agcccggcga tgaacgaaat
     6541 ggtaaaagag cgtttggttt taggcgcagc gctgaaagaa gcgattagca ataaccaact
     6601 gaaactggtt taccagccgc aaatcttcgc agaaacgggt gaactgtacg gcatcgaagc
     6661 ccttgctcgc tggcacgatc cccagcatgg tcatgtaccc ccttcacggt ttattcctct
     6721 cgcagaagag attggtgaaa tcgaaaatat tgggcgctgg gtcatcgcgg aagcttgccg
     6781 tcagttagca gaatggcgta gccagaatat tcatatcccg gcgttatccg tgaacttgtc
     6841 ggcgctgcac tttcgcagta atcagctgcc taatcaggtg tctgatgcaa tgcaagcctg
     6901 gggtattgac ggccaccagc tgacggtaga aatcacggaa agcatgatga tggaacacga
     6961 taccgaaatc tttaagcgca ttcagatcct gcgtgatatg ggcgtgggct tatcggtaga
     7021 tgattttggt acgggctttt ccggattatc ccgcttagtc agtcttccgg taacggaaat
     7081 caaaattgac aaaagttttg tcgatcgttg tctgaccgaa aaacgcatcc ttgccttact
     7141 tgaagccatt accagcattg ggcaaagcct caatttaacc gtcgtggcgg aaggcgtcga
     7201 aaccaaagag caatttgaga tgctacgcaa gatccactgt cgcgttattc agggatattt
     7261 cttttcccgc cccctacccg ccgaagaaat tccaggctgg atgagcagcg tgttaccgct
     7321 gaaaatctga caaattcctc tcgcccgcac tcgcgggttt tcatttaacg tgacactgtc
     7381 acttaaatac tgtgatttca gctacgattc cgggagattc cttccttaac ttcccctctt
     7441 gcaccaaaac tgcactacgc tgcacaaatg ttgatcaaaa gttaaaaaat ataactttaa
     7501 gccagcattc tggcgcttat cccggcatgg catgagatct gcataagcgg aaagcgcagc
     7561 aatttttgtc ttatacagga accgtttatg tcggatacca ccgaactggt tgatttagcc
     7621 gtaatcttcc ctgatctgga gatcgaattg aaatacgcct gcgctgataa catcacaggt
     7681 aaagctattt atcagcaagc gcgttgtctg ctacacaagg atgcgattac cgcgctggcg
     7741 aaaagtatca gtatcgccca gctgtcaggc ttacaactgg tgatttacga tgcgtatcgc
     7801 ccacaacaag cacaggcgat gttgtggcaa gcctgcccag acccgcaata tgttgttgat
     7861 gtgacggtcg gttctaatca cagccgtggc acggcgatcg acctgacgct tcgtgatgag
     7921 cacgggaaca tcctcgatat gggcgcaggg ttcgatgaaa tgcacgagcg ctcccatgcc
     7981 tatcaccctt ccgtcccgcc cgccgctcag cgcaatcggc tgttgctgaa tgcgataatg
     8041 actggtggcg gttttgtcgg tatctccagc gaatggtggc acttcgaatt accccaggca
     8101 gcgagttacc ctctgctcgc tgatcaattc tcttgtttta tatcccctgg cacacagcac
     8161 gtcagttaat caggaaactg tcatgaagag atcgatatcg tttcgtccca cattgctcgc
     8221 gctcgtcctt gccacaaatt tcccggtttc gcacgccgcc gtaccgaaag atatgctggt
     8281 gattggtaag gccgccgatc cacaaaccct cgacccggcg gtaacaatag ataataacga
     8341 ctggacggtg acctacccgt cttatcagcg gctggttcag tacaaaacgg acggtgataa
     8401 aggctcaacc gacgttgaag gcgatctggc aagtagctgg aaagcgtctg acgatcaaaa
     8461 agagtggacg ttcaccctga aagataatgc taaatttgcc gatggcacac ctgtcactgc
     8521 cgaagcagta aaactttctt ttgagcggct actaaaaatc ggccaggggc cagcagaagc
     8581 atttcccaaa gatttaaaga ttgatgctcc cgacgaacat acggtgaagt ttacccttag
     8641 ccaaccattc gcaccgttcc tctacacgct ggcgaatgac ggtgcatcca ttatcaatcc
     8701 ggcggtctta aaggaacatg cagcggatga tgctcgcggc ttcctcgcgc aaaataccgc
     8761 cggttccgga ccatttatgc tgaaaagctg gcaaaaaggt cagcaattag ttctggtgcc
     8821 aaatccgcat taccccggca ataaaccgaa ctttaagcga gtatcggtaa aaattatcgg
     8881 tgaaagtgcc tcccgtcgcc tgcagctctc ccgtggcgac attgacattg ccgatgcgct
     8941 gccggtggat caactcaacg ccctgaagca ggaaaataaa gtcaatgtgg cagagtatcc
     9001 gtcactgcgc gttacctatc tgtatctgaa taacagcaaa gcgcctctta atcaggcgga
     9061 tctacgtcgg gccatttcct ggtctaccga ttatcagggc atggttaacg gcattctgag
     9121 tggtaacgga aaacagatgc gcggcccgat tccggaaggc atgtggggct acgatgcgac
     9181 ggcaatgcaa tacaaccatg acgaaacgaa agccaaagcc gaatgggata aagtgacgag
     9241 caaacccacc agcctgacgt ttctctactc cgataacgat ccgaactggg agcctattgc
     9301 tttggcgaca caatccagtc tcaacaagct gggtatcaat gtgaagctgg aaaagctggc
     9361 gaacgccacc atgcgcgaca gagtgggtaa aggtgattac gacattgcga ttggcaactg
     9421 gagtccggat tttgccgacc cgtatatgtt tatgaattac tggtttgagt cagacaaaaa
     9481 aggtctgccg ggtaaccgct cgttctatga aaacagtgag gtcgataagt tactgcgcaa
     9541 tgcgcttgcg accaccgacc agacgcagcg tacccgggac taccagcagg cacagaaaat
     9601 cgtcattgat gacgctgctt atgtgtacct gttccagaaa aactaccaac tggcgatgaa
     9661 caaagaggtg aaaggctttg tgttcaatcc catgctggaa caggtcttca atatcaatac
     9721 catgagtaaa taacgtgacc ttctggagta ttttacgcca acgctgctgg gggctggtgc
     9781 tcgtggtggc gggcgtctgc gtgattacgt ttattatctc gcatctgatc cctggcgatc
     9841 cggcgcggtt actggcgggt gaccgcgcca gcgatgctat cgtggaaaat attcgccagc
     9901 aactgggact ggaccagcca ctgtatgtac agttttaccg ctacgtcagc gacctgtttc
     9961 atggtgacct gggaacatcc attcgtaccg ggcgtccggt gctggaagag ttgcgtatat
    10021 ttttcccggc gacgctggag ctggcttttg gtgccctgct gctggcactc ctgattggca
    10081 tcccgctggg catactctct gcagtctggc gaaatcgctg gctggatcat ctggtgcgaa
    10141 taatggccat taccggaatc tccacacctg cgttctggct tgggctgggc gtcattgtgc
    10201 tgttttatgg tcatctgcaa attcttcccg gcggcggaag gcttgatgac tggctggatc
    10261 caccaacgca cgttaccggc ttttatctgc tcgatgcgct gcttgaaggc aacggtgaag
    10321 tcttcttcaa tgcgttgcaa catctcatct taccggcatt aacgctggcg ttcgttcacc
    10381 tgggaattgt cgctcgccag atccgctcag cgatgctgga acaattgagt gaagactaca
    10441 ttcgtaccgc ccgggccagc ggcttgcccg gctggtatat cgttttatgt tatgcgctac
    10501 ccaatgcgtt gatcccatcg attaccgtat tgggtttggc gctgggcgat ttgttgtatg
    10561 gcgcagtgct caccgaaacc gtttttgcct ggcccggaat gggtgcatgg gtagtaacat
    10621 caatacaggc gctcgacttc ccggcagtga tgggctttgc cgtcgtggtt tcatttgctt
    10681 atgtgctggt caacctggtg gtggatttgc tctatttgtg gattgatccg cgtatcggac
    10741 gtggaggtgg tgaatgatgc taagcgagga aacgtccgcc gtacgcccac aaaaacaaac
    10801 gcgatttaac ggtgcaaaac tggtatggat gctgaaaggc agtccgctca ccgtgaccgg
    10861 cgcagtcatc attgtattaa tgctattgat gatgattttt tcaccgtggc tggcgacgca
    10921 tgatcccaac gccattgatt taaccgcccg ccttttgccg ccttctgcgg cgcactggtt
    10981 tggcaccgat gaagtgggac gcgatctgtt cagccgcgta ctggtcggca gtcagcaatc
    11041 aattctcgcc ggattagtcg tggttgccat tgcgggtatg attggttcgc tactcggatg
    11101 tctatccggt gtgcttggcg gacgcgcaga cgccatcatt atgcgcatca tggacattat
    11161 gctgtcgatt ccttcgttgg tactgacaat ggcactggct gccgctctcg ggccgagttt
    11221 atttaacgcc atgctggcgc ttgctattgt gcgaattccc ttttatgtgc gcctggcgcg
    11281 ggggcaagca ttagttgtac gccagtatac ctatgttcag gcggcgaaaa cctttggtgc
    11341 gtcgcgttgg catctgatca gctggcatat tttacgtaac tccctgccgc cgctgatcgt
    11401 gcaggcatcg ctggatatcg gtagcgcgat tttaatggcc gccacgttgg gatttattgg
    11461 cctgggtgct caacaaccga gtgctgaatg gggcgcgatg gtagcgaatg gtcgcaacta
    11521 tgtactcgat cagtggtggt attgcgcatt tccgggggca gcgattttgc ttaccgccgt
    11581 tgggtttaat ctctttggtg atggtattcg cgatctgctt gacccgaaag caggaggaaa
    11641 gcagtcatga cccaacccgt tctggacatt caacagctgc atttgagttt ccccggtttt
    11701 aacggtgatg ttcacgcgct caacaatgtg tccttgcaga ttaaccgcgg cgaaattgtc
    11761 ggtctggtgg gagaatccgg ctcaggtaaa tcagtcaccg caatgctgat tatgcgtctg
    11821 ctacagacgg gcagttattg cgtacatcgg gggcagattt cactgctggg agacgatgtt
    11881 cttaacgctc gggaaaaaca gcttcgtcag tggcgcggcg cacgagtggc gatgatcttt
    11941 caggaaccga tgaccgccct caatccgaca cttcgaatag gtcttcagat gatggacgtg
    12001 atccgccatc atcaaccaat aagtcgtcgg gaagccagag ctaaagcgat tgccctgctg
    12061 gaagagatgc aaatcccgga tgccgtggaa gttatgtcgc gctatccgtt tgagctttca
    12121 ggtggtatgc gccagcgggt aatgattgcg ctggcattct cctgcgagcc gcaattgatt
    12181 attgccgacg aaccgactac ggcgctggac gtcacggtgc agttgcaggt actgcgtttg
    12241 cttaaacata aagcccgcgc cagtggaact gcggttctgt tcatcagcca tgatatggcc
    12301 gtggtgtcgc aactgtgcga tagcgtttac gtgatgtatg ccggaagcgt aattgaaagc
    12361 ggcgtgacgg cagatgttat ccatcatccc cggcatccgt ataccattgg tttgctccaa
    12421 tgcgcaccag aacatggagt accacgccag ctattacccg ccattccagg gacggtacca
    12481 aacctcaccc atttgcctga aggctgcgct tttcgcgatc gttgctatgc ggcaggtgca
    12541 cagtgtgaaa acgtcccggc gctgacagcg tgtggtgaca acaaccagcg ctgcgcctgt
    12601 tggtatccgc agcaggaggt cataagtgtc tgacacgtta ttaacgttac gcgacgtcca
    12661 tatcaatttc ccggcccgta aaaactggct tggtaaaact acggaacatg ttcatgccat
    12721 taatggtatt gatttacaga tccgccgtgg tgaaacctta gggatcgtcg gcgagtcagg
    12781 ctgcggcaaa agcaccctcg cacagctttt aatgggcatg ctgcaaccga gccacgggca
    12841 gtacatccgt tctggctcac aacgcattat gcagatggtg tttcaggacc cgctctcttc
    12901 gctcaatccg cgcttaccgg tgtggcgcat catcacagaa ccgctctgga tagctaagcg
    12961 tagtagtgaa caacagcggc gagcgttggc agaggagctg gctgtgcagg tgggtattcg
    13021 tccggagtat ctcgaccgcc tgcctcatgc gttctccggc gggcagcggc aacgcatcgc
    13081 cattgccaga gcactctctt cgcagcctga cgtgattgtg cttgatgagc caacctcggc
    13141 gctggatatc tccgtgcagg cgcagatcct caatttactg gtaacgctac aggaaaatca
    13201 cgggctgact tatgtgctga tttcacacaa tgtctcggtg atacgtcata tgagcgatcg
    13261 ggtggcggtg atgtatctcg ggcagattgt ggaactaggg gaagcgcaac aggtgttgac
    13321 tgttccggca catccatata cccgattatt gctggattcc ctccccgcca ttgataaacc
    13381 gctggaggaa gaatgggcat tacgtaaaac ggatctgccg ggaaaccgca cgttgccgca
    13441 aggctgtttt ttctacgaac gttgccctct ggcaacccac ggatgtgaag tccggcaatc
    13501 attaacaata agggaggacg gacgtgagat ccggtgctgg cgggcgctgt agagatgcta
    13561 acaacgcatc aggcattcac gcgcctgacg cgtcatccgg caatgcttta cgatttcaac
    13621 tggtaatcaa gcgtaatttc cgctttcagt acctgagaaa ccgggcatcc tgcttttgct
    13681 ttctggatga tgccgtcaaa ggtagaggca tcaatacccg gcaccgcaac ttcactcttc
    13741 agtgcgattt tcgtaatcgc aaaaccggca tccactttat ccagcgacac atcggcggtg
    13801 gtatcaatcg atgttggcgt aaatcccgct tcccccagca ttaatgaaag cgccattgag
    13861 aaacatgcgg catgcgctgc gccaatcagt tcttcagggt tagttccttt ttcgccttca
    13921 aaacgcgtgt taaatccata cggctgttgg ttcagcacac cactctcggt ggatactgtt
    13981 cccttcccgc gtttgatatc gccttcccag tgtgcctgac ctttcttatg gattgtcatt
    14041 gttgctctcc tgtgggctta aaaaggaaat tatagactac gtgagaaaca gcacgaggat
    14101 aagcaggata ttccgaataa aaatccggca gaaatattac ttctgcactt aaattaaatt
    14161 tccacaatag ataaccggga atatataacc caagagatat tgcattaata ttaatcaact
    14221 caccacaata ataataccta tcaatatcat gaggttaaaa tataaaaatc atcaaccagg
    14281 actaatctta acaacgaagc ggcaaatatt tgccatgctg aatgtgcttt ataaatctgc
    14341 gatccgtagc agacaccata aatacacaga cacggagaat cactatgttt acttattatc
    14401 aggcagaaaa ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg
    14461 cagagcacgg tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag
    14521 cttctgataa cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg
    14581 gccagcaccc tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc
    14641 acgtgtttct tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa
    14701 aatgaaatcg aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca
    14761 gcgcaaaata gttaccgaag gtgacaaatc cagcgtggta aataatccaa ccggcagaaa
    14821 acgccccgct gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc
    14881 ctgcgcaatg gtgtttttgt ttttatctgc tctatacttg gggccgacgc cctggcggta
    14941 aagcaaagac gataaaagcg tgccagggat ggatattcaa aaaagagtga gtgacatgga
    15001 accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga
    15061 atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct
    15121 gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat
    15181 ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga
    15241 caccaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt
    15301 tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc
    15361 acgcggcgtg tttatctctt accagaaccg gcacaatatg gacgatattc tgcaaaacgt
    15421 gccgaaccat aatattaaag tgattgtggt gactgacggt gaacgtattc tggggcttgg
    15481 tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg
    15541 tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggaa cgaacaacca
    15601 acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccacgtatca ctgacgatga
    15661 atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc cggacgtgct
    15721 gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa
    15781 tgaaatttgt tcttttaacg atgacattca gggcactgcg gcagtaacag tcggcacact
    15841 gatcgcagcc agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg tcttccttgg
    15901 cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc agcgcgaagg
    15961 attaagcgag gaagcagcgc ggcagaaagt ctttatggtc gatcgctttg gcctgctgac
    16021 ggacaagatg ccgaacctgc tacctttcca gaccaaactg gtgcagaagc gcgaaaacct
    16081 cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc gcaatgtaaa
    16141 accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag agatcattcg
    16201 tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg
    16261 cgtggaagcc acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc tggtcgccac
    16321 tggcagcccg tttaatccag tggtatggaa agataaaatc taccctatcg cccagtgtaa
    16381 caacgccttt attttcccag gcatcgggct gggtgttatt gcttccggcg cgtcacgtat
    16441 caccgatgag atgctgatgt cagcaagtga aacgcttgct cagtattcgc cgctggtgct
    16501 gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct cccgcgcaat
    16561 tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa cgtctgccga
    16621 agctctgcaa caagccattg acgataattt ctggcaagcc gaataccgcg actaccgccg
    16681 tacctccatc taagcctgcg cccggtagtg aaggctaccg ggctatttcc ctctcccttt
    16741 ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca cctgctatgc
    16801 agaacatcat ccgaaaagga ggaactatga aggctgcagt tgttacgaag gatcatcatg
    16861 ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg
    16921 agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt ggtgacaaaa
    16981 ctggggtaat tctgggccat gaaggcatcg gtgtggtggc agaagtgggt ccaggtgtca
    17041 cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ctacgaagga tgcggtcatt
    17101 gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat gccggataca
    17161 gcgttgatgg cgggatggcg gaagagtgca tcgtggtcgc cgattacgcg gtaaaagtgc
    17221 cagatggtct ggactcggcg gcggccagca gcattacctg tgcgggggtc accacctaca
    17281 aagccgttaa actgtcaaaa attcgtcccg gtcagtggat tgctatctac ggtcttggcg
    17341 gtctgggtaa cctcgccctg caatacgcga agaatgtctt taacgccaaa gtgatcgcca
    17401 ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat gggtgcagat ttagcgatta
    17461 actcacgcac cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc gctcacgctg
    17521 cggtggtaac agcagttgct aaagctgcgt ttaactcggc agttgatgct gtccgtgcag
    17581 gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat gagcctggat atcccacgcc
    17641 ttgtgctgga tggcattgaa gtggtcggtt cgctggtcgg cacgcgccag gatctaaccg
    17701 aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg cgtccgttag
    17761 cggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc cgtatggtga
    17821 ttgatttccg ccgttaagag gcctttgctg cgagtgccag tcctggtgtc gcagcatcgc
    17881 actctctccg acattaaatg gctaaatgga ttattgttcc ttcctacgca acaataatga
    17941 tagtgttcct gatgaaggaa cactactgag agtaaagaca ctgatgaaaa tggccaatca
    18001 tccccgcccg ggggacatta ttcaggaatc actggacgaa cttaatgtca gcctgcgcga
    18061 gtttgccaga gcaatggaaa ttgctccctc aacggcaagc cgattgctga ccggaaaagc
    18121 agctttgaca ccagaaatgg cgattaaact ttccgtggtg atcggcagtt cgccgcaaat
    18181 gtggctgaat ctgcaaaacg cctggagtct ggcagaggca gaaaaaacgg tggatgtgtc
    18241 gcgactacgc cgtctggtta cgcaataaaa aaatggcgct tcttaaagcg ccattttcag
    18301 cttaaagttc aaaagagatt atatcccttc ttcactctct tttttcgctt ctgccttctc
    18361 gatttcacga taccagcgcg gatggtgttt cttcgcccag cgacggctca ccttcccttc
    18421 gatcatccct ttaatcgacc ctttcaccca gaatgccata tacatatgga tcaggatggc
    18481 gtggatcagg atgatacccg cagccgcgtg gatcagcagg ctgtagcgaa caacctgcat
    18541 cgggaagtac tgcgcaaagt acggacgcca gataatcacc ccggtcacca gcagcacgaa
    18601 aatcatgctc atgatcgacc agaacatcat cttttgcccg gcgttgtact taccgacatc
    18661 cgccacttta tgctcattgc ctttcaatac ttcgacaatg ttcaacagcc acggaatatc
    18721 tttcttatcc gggatgttgt gatgcacaaa acgcacaaac ataaacatca gtgcgacgaa
    18781 aatcgcaatg ccgaaaaacg ggtgcaaaat gcgtcccatc tgcggcgtac cgaaggtttg
    18841 cgtcagccat tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca ccaggaagaa
    18901 gcaaatcacc acggtccagt gacaggcgcg atcaataaat ttggtgcgca caatcatttt
    18961 cgacttactc atgatgatcc tcctcgtcat cgtccacttc cttattcggg ccaataccga
    19021 tgtagtggaa aatcaacccg gcaaaagtgg caataaagcc agccgctgcc agcggtttca
    19081 acgcgccttt ccacaggctt accgaggtgt cgatcttcgg atctttcggc agaccgtgat
    19141 acagctccgg ctgatcggcg tgatgcagca cgtacataac gtgcgtacca ccgacccctt
    19201 ccgggttgta gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc acgcgctgtt
    19261 ccgccagctc cagcatctcc ttcttggtgc cgaagtggat agccccggtc ggacaggttt
    19321 tcacacaagc cggttcctgg ccgacgctga cgcgatcgac gcagagcgtg catttatata
    19381 cccggttatc ctctttgttg aggcgcggaa tattaaacgg acacccggca atgcagtaac
    19441 cacagccgat gcagttttcc gactggaaat cgacaatccc gttagcgtac tgaatgattg
    19501 caccagcaga cgggcacgcc ttcaggcagc cgggatcttc acagtgcata cagccgtctt
    19561 tacggatcag ccactccagc ttgccgttct gttcggtttc gctaaagcgc atcaccgtcc
    19621 aggacttggc gctcagatcg gcggggttat cgtaaacccc gacgcagtgc cccacttcat
    19681 cacggatgtc gttccactcc gaacacgcca cctgacaggc tttacagccg atacaggtgg
    19741 aaacgtcgat aagttttgcg acttctgctt tgtaatcacg cacctgagaa ggcggcgtga
    19801 tggagttagt tgcggacctt ttgataatgt cctgcgtttc catagccatc tgttcgcccc
    19861 cttacgcctt ctcgatgttg actaagaacg ctttatattc cggcgtttgc gagtttgcat
    19921 caccgacatt cggcgtcaga gtgttagcga tataaccttt acgcgcgaca ccctcaaagc
    19981 cccagtggat tggaataccc accgtttcaa cctgctgacc atttacattc agtggtttca
    20041 gacgacgcgt taccacagcc accgcgcgga taaagccacg cttgcttgag acagtgacac
    20101 gatcgccatt attaatgcct ttcgccgccg ccagcgtttc gctgatttcc acaaactgtt
    20161 ccggctgagc aattgcgttg agcaatgcgt gcttggtcca ggtgtggaag tgctcggtca
    20221 gacgataggt cgtacccaca tacgggaact gctctttttt acccatccgc agtgcgtctt
    20281 gttcatacag acgaacaacc gggttagaca ccacgttcgg atgcagcggg ttagtgccca
    20341 gcggcgtttc aatcggctcg tagtgttccg ggaacggacc ttccgccatt ttgttgatgg
    20401 caaacaggcg tcccatccct tccggctgca tgataaacgg cccggttggc gtacccggtg
    20461 cggcattgcc gaagtcagga atatcgttac ccgtccactt gctgccgttc cactggatca
    20521 gcatccgttt cggatcccac ggtttaccgt tgatatccgc cgaagcacgg ttgtacagca
    20581 cgcgacggtt gagcggccac gcccaggccc atcccagcgt attccccaga ccggacgggt
    20641 cggagttatc gcggttagcc atctggttgc cctgctctgt ccagctaccg gtgtagatcc
    20701 agcaagaaga tgcggttgta ccgtcatcac gcagatgcgc aaagctactc agcaactgac
    20761 ctttcttcgc aatcagcacg ccattagcat catagagatc ttccagcgca tagccgttgt
    20821 tctctttagc tacttcgtca gattgcggtt cgtgcggctg cttgtagttc cagctcatct
    20881 tcatcagcgg ttctacgcct ttaccacctt cggcctggta cagctcgcgc aggtgatggt
    20941 agatacccgc cagaatttca ccgtcgttac gcgcttcgcc cggcgcatcc tgacctttcc
    21001 agtgccactg cagccagcga ccggagttag caatagaacc atcttcttca gcaaagcagg
    21061 tcgaaggcag acggaatact tcagtctgaa tagacgccgg atcgacatcg ttcgactcgc
    21121 cgtggttctg ccagaaggtt gaggtttcag tcaccagcgg atcgataacc accatgtact
    21181 tcagcttgct taggcagctc accactttgt ttttgtccgg gaaggacgca accgggttaa
    21241 agccctggca gaaataaccg gtgactttgc cttcatccat catgttgaaa tacttgatga
    21301 cgtcgtaggt ctggtcccac ttcggcagcc agtcatagcc ccagttgttc tctttctgcg
    21361 cggcatcgcc atagaaagat ttcatcaggc taacgaagaa cttcggatag ttgctccagt
    21421 agttcacctg accagccagc gtcgctttcg gcgtgttcgc ttccagatac gactgcaaat
    21481 caacctgttt ttctgacggc agcgtcagat aacctggcag gctggtagag agcagaccta
    21541 agtcagtcag accctgaatg ttggagtgac cacgcaatgc attcacgccg ccaccggcca
    21601 tacccatgtt accgagcagc aactggatca tcgccatagt acggatgttc tgcgcaccca
    21661 cggtgtgctg cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc ggtgcgctgg
    21721 tggaggccag cacttcacac actttcagga agtcggcttt tggcgtacca cagatgtttt
    21781 caacaacgtc cggcgtgtag cgggaaacgt gcgctttcag caggttccac acacagcgcg
    21841 gatgagtcag tgtttcatcg cgtttcgcat agccgttttc atcgaactga tagttccagg
    21901 acgatttatc gtattgccgt ttttcagcgt cgtagccgct gaacagaccg tcttcgaaag
    21961 caaaatcatc acgcaccagc aggctggcgt tggtgtaatg cttaacgtat tcggcgttga
    22021 ttttgtcgtt ttcgatcagg tagcgcaaaa cgccagacag gaacgtaatg tccgtaccgg
    22081 aacgaatagg tgcgtaaata tccgccacag aagcggtacg cgtaaaacga ggatcgacaa
    22141 cgatcaaggt tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa ccgacgggat
    22201 gcgcttcagc agcgttaccg cccattacca ttacgacgtt agcgtttttg atatccaccc
    22261 agtggttggt catcgcaccg cgaccaaatg ttggagcaag acttgctacc gttggtccgt
    22321 gtcagacgcg cgcctggttg tctaccgcca gcatcccgag ggagcgggca aatttctgtg
    22381 tcagcatccc ggtttcgttg ctggcaccgg aggcacacag cataccggta gaaagccaac
    22441 ggtttaccgt tacgccctgc tcgttctttt caataaagtt agcgtcacgg tcagctttca
    22501 tcagctttgc aatacgggag aatgcttctt cccagctaat gcgctgccat ttgtcagaac
    22561 ctggcgcacg atattccggg tagcgcagac ggttttcgct gttgacgtaa tccagcaaac
    22621 cggccccttt tgggcacagc gcaccacggc ttaccggatg atccgggtca ccttcaatgt
    22681 gataaatcgc ttctctggcg tttttcgcgc catcacccag gctatacatc aatagcccgc
    22741 aacctacgga acagtatgtg caggtgttac ggatctcttt agcgcgtaat aatttgtagt
    22801 ttcgcgcctg agccagtgct tgcttcgggg caaagcccaa tgccgctacc gttgttccag
    22861 ccataccgcc cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt gctttcctct
    22921 tttttcaggg gtattactgc gcgaggaaac taacagtaaa attctgaatc tttttgcgcg
    22981 aaatcaaaaa gaacacgtgt ttgccacaaa ataatccgcc tgcgcaaatt gacctacctc
    23041 aatagcggta gaaaaacgca ccactgcctg acaggccagt taaaaaaatg ctataaaatt
    23101 cagcttaatt tttaacggca agagagacaa aacagcgagc atgacacgac aaaaagcaac
    23161 gctcataggg ctaatagcga tcgtcctgtg gagcacgatg gtaggattga ttcgcggtgt
    23221 cagcgagggg ctcggcccgg tcggcggcgc agctgctatc tattcattaa gcgggctgct
    23281 gttaatcttc acggttggat ttccgcgtat tcggcagatt ccgaaaggct atttactcgc
    23341 cgggagtctg ttattcgtca gctatgaaat ctgtctggcg ctttccttag ggtatgcggc
    23401 gacccgtcat caggcgattg aagtgggtat ggtgaactat ctgtggccca gcctgacaat
    23461 tctctttgcc attctgttta atggtcagaa aaccaactgg ctgattgtac caggattatt
    23521 attagccctg gtcggcgtct gctgggtgtt aggcggtgag aatgggttac attatgatga
    23581 aatcatcaat aatatcacca ccagcccatt gagttatttc ctggcgttta ttggtgcgtt
    23641 tatctgggca gcctattgca cagtaacgaa taaatacgca cgcggattta acggaattac
    23701 cgtttttgtc ctgctaacgg gagcaagtct gtgggtttac tattttctca cgccacaacc
    23761 agaaatggta tttagcacgc ccgtcatgat taaactcatc tctgcggcat ttaccttagg
    23821 atttgcttat gctgcatgga atgtcggtat actgcatggc aatgtcacca taatggcggt
    23881 aggctcgtat tttacgcccg tactttcctc agcgcttgca gccgtgctgc tcagcgcccc
    23941 gctgtcgttc tcattctggc aaggcgcgct aatggtctgc ggcggttccc tgctctgctg
    24001 gctggcgaca cgtcgtggtt aaaataattg ccgggtctac ggcccggcag ttaatcagat
    24061 aaattccgac aatattaaaa aataaatttc aaataccagt aattcacctt a
//
LOCUS       DAZGOB010000067        24073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGOB010000067 DAZGOB010000000
VERSION     DAZGOB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.067"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..384)
                     /gene="sbmC"
                     /locus_tag="ACHLIF_002870"
     CDS             complement(<1..384)
                     /gene="sbmC"
                     /locus_tag="ACHLIF_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO9606860.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDIVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPK"
     gene            complement(502..1668)
                     /gene="dacD"
                     /locus_tag="ACHLIF_002871"
     CDS             complement(502..1668)
                     /gene="dacD"
                     /locus_tag="ACHLIF_002871"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO9606861.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRTIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHDEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            1877..3304
                     /gene="sbcB"
                     /locus_tag="ACHLIF_002872"
     CDS             1877..3304
                     /gene="sbcB"
                     /locus_tag="ACHLIF_002872"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO9606862.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQETRAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(3347..3574)
                     /gene="tsuB"
                     /locus_tag="ACHLIF_002873"
     CDS             complement(3347..3574)
                     /gene="tsuB"
                     /locus_tag="ACHLIF_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO9606863.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(3588..4646)
                     /gene="tsuA"
                     /locus_tag="ACHLIF_002874"
     CDS             complement(3588..4646)
                     /gene="tsuA"
                     /locus_tag="ACHLIF_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO9606864.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(4825..6183)
                     /gene="plaP"
                     /locus_tag="ACHLIF_002875"
     CDS             complement(4825..6183)
                     /gene="plaP"
                     /locus_tag="ACHLIF_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO9606865.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(6173..6235)
                     /gene="yoeI"
                     /locus_tag="ACHLIF_002876"
     CDS             complement(6173..6235)
                     /gene="yoeI"
                     /locus_tag="ACHLIF_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO9606866.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(6450..7379)
                     /gene="yeeY"
                     /locus_tag="ACHLIF_002877"
     CDS             complement(6450..7379)
                     /gene="yeeY"
                     /locus_tag="ACHLIF_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9606867.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(7425..8249)
                     /gene="yeeZ"
                     /locus_tag="ACHLIF_002878"
     CDS             complement(7425..8249)
                     /gene="yeeZ"
                     /locus_tag="ACHLIF_002878"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9606868.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(8332..8586)
                     /gene="yoeB"
                     /locus_tag="ACHLIF_002879"
     CDS             complement(8332..8586)
                     /gene="yoeB"
                     /locus_tag="ACHLIF_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO9606869.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(8583..8834)
                     /gene="yefM"
                     /locus_tag="ACHLIF_002880"
     CDS             complement(8583..8834)
                     /gene="yefM"
                     /locus_tag="ACHLIF_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO9606870.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            9117..9164
                     /gene="hisL"
                     /locus_tag="ACHLIF_002881"
     CDS             9117..9164
                     /gene="hisL"
                     /locus_tag="ACHLIF_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO9606871.1"
                     /translation="MTRVQFKHHHHHHPD"
     misc_feature    9140..9263
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            9310..10209
                     /gene="hisG"
                     /locus_tag="ACHLIF_002882"
     CDS             9310..10209
                     /gene="hisG"
                     /locus_tag="ACHLIF_002882"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO9606872.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            10215..11519
                     /gene="hisD"
                     /locus_tag="ACHLIF_002883"
     CDS             10215..11519
                     /gene="hisD"
                     /locus_tag="ACHLIF_002883"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO9606873.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRQDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            11516..12586
                     /gene="hisC"
                     /locus_tag="ACHLIF_002884"
     CDS             11516..12586
                     /gene="hisC"
                     /locus_tag="ACHLIF_002884"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO9606874.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPAAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            12586..13653
                     /gene="hisB"
                     /locus_tag="ACHLIF_002885"
     CDS             12586..13653
                     /gene="hisB"
                     /locus_tag="ACHLIF_002885"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO9606875.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGDSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            13653..14243
                     /gene="hisH"
                     /locus_tag="ACHLIF_002886"
     CDS             13653..14243
                     /gene="hisH"
                     /locus_tag="ACHLIF_002886"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO9606876.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFALPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            14243..14980
                     /gene="hisA"
                     /locus_tag="ACHLIF_002887"
     CDS             14243..14980
                     /gene="hisA"
                     /locus_tag="ACHLIF_002887"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO9606877.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     LLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVESY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            14962..15738
                     /gene="hisF"
                     /locus_tag="ACHLIF_002888"
     CDS             14962..15738
                     /gene="hisF"
                     /locus_tag="ACHLIF_002888"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO9606878.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCRVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            15732..16343
                     /gene="hisIE"
                     /locus_tag="ACHLIF_002889"
     CDS             15732..16343
                     /gene="hisIE"
                     /locus_tag="ACHLIF_002889"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO9606879.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(16440..17417)
                     /gene="wzzB"
                     /locus_tag="ACHLIF_002890"
     CDS             complement(16440..17417)
                     /gene="wzzB"
                     /locus_tag="ACHLIF_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO9606880.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(17564..18730)
                     /gene="ugd"
                     /locus_tag="ACHLIF_002891"
     CDS             complement(17564..18730)
                     /gene="ugd"
                     /locus_tag="ACHLIF_002891"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO9606881.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPNTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTKNIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(18979..20385)
                     /gene="gndA"
                     /locus_tag="ACHLIF_002892"
     CDS             complement(18979..20385)
                     /gene="gndA"
                     /locus_tag="ACHLIF_002892"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO9606882.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(20496..20903)
                     /locus_tag="ACHLIF_002893"
     CDS             complement(20496..20903)
                     /locus_tag="ACHLIF_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFO9606883.1"
                     /translation="MRLVDDNIMDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGELQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
     gene            complement(20894..22102)
                     /locus_tag="ACHLIF_002894"
     CDS             complement(20894..22102)
                     /locus_tag="ACHLIF_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO9606884.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDNT
                     LQAIYSVSMTDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWHALKHLIQHDTFDGI
                     VYYSPSIFWGGLVKKIKERCQCPSYLVLRDMFPQWVIDAGMIKAGSPIEKYFRYFEKK
                     SYQQADWIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVSAGDDYHSLRQKYALKDK
                     IIFFYGGNIGHAQDMANLMRLARNMMRHHDAHFLFIGQGDEVDLIKSLSAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     gene            complement(22102..23232)
                     /gene="wecB"
                     /locus_tag="ACHLIF_002895"
     CDS             complement(22102..23232)
                     /gene="wecB"
                     /locus_tag="ACHLIF_002895"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO9606885.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVFEQEKPEAMLVLGDTNSCI
                     SAIPAKRRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMSQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAEKYDIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNAR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESARVLQALEIIATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            complement(23229..>24073)
                     /locus_tag="ACHLIF_002896"
     CDS             complement(23229..>24073)
                     /locus_tag="ACHLIF_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO9606886.1"
                     /translation="IMLSSSIQAECDNAYGKSKAVAEKIIQQYGETTNAKYYIYRLPN
                     VFGKWCQPNYNSFIATFCHRIANDEAITINDPSAVVNLVYIDDFCSDILKLLEGANET
                     GYRTFGPIYSVTVGEVAQLIYRFKESRQTLIIEDVGNGFTRALYSTWLSYLSPKQFAY
                     TVPSYSDDRGVFCEVLKTKNAGQFSFFTAHPGITRGGHYHHSKNEKFIIIRGSACFKF
                     ENIVTGERYELNVSSDDFKIVETVPGWTHDITNNGSDELVVMLWANEIFNRSEPDTIA
                     RVLS"
BASE COUNT         6395 a         6046 c         5683 g         5949 t
ORIGIN      
        1 ctttggtaac atttcataag cactgtcctg caagaggcta ttaaagaact gataccaggg
       61 tttagcaaaa tcatcaccga ctacacgagc caccgcaacc gcatactgac cacctgaaat
      121 ttctgtcaga atgacgccct cactgttttc ggggagcgta aagttattcg gcaccgtcac
      181 gacgatgtcg cagcgtaatt tttcggcggg tgtttcatct ggattgtcgt aaaagacagc
      241 aacccactcc ttcggcacaa tatttttgct atctacccac atcatcaact gctcaaagcc
      301 tttctttacc gtctgttccc acgggccaac gagatggaaa cctgcaacgg tacgtttatc
      361 ttcctgctta atctcgtagt tcatgacgtc ccattaacac tgtttttata tacagtataa
      421 ttgcgatatt aaaaccacaa ggaatgagtg ttgattatgc gagcagactc gcactcctgc
      481 cagtctgctg caaaagaaag gtcaggcctt atggtggaaa taatcactca ggcgagaaaa
      541 catgctgcct tccccgacag attccagggt aaccagcggc cagtgcgcca cctgtttatc
      601 acggtcgtaa agttcaattt cccctacccg ctgatgggcg ctaattggcg cggtgagctc
      661 tttaccatca agggtatatt tggctttgat atgtggaatt tcggctttcg gtagcaccat
      721 ccagaactct tgttccgttc ccaggtcgat attttcttta tcgccatacc agatgcgttc
      781 cgtaccgacc tttttcccac ggtgcaaaat ttgcaccgta gtaaagtttt gttgccccca
      841 acgcagtaat tttcttgcct cttcctcacg accttttgca ctgtcagccc ccattaccac
      901 tgcaatgaga cgacgctgcc catcgacagc cgaagcaatg agattaaacc cggcaccaga
      961 agtatgaccc gttttcaggc cgtcaacatt catggtttta tcccacaata acccgttacg
     1021 gttttgctgg gtgataccgt tccaggtgag acttttctca ctgtacatat gataaaactc
     1081 gggctcgtcg tggatgatag cgcgagaaag cacagctaaa tcataagccg agctatgctg
     1141 acctggtgca tccagaccat gcactgtttc aaaatgcgta tccttgagat gcagcttctc
     1201 ggcatagttg ttcatcattt caacaaactg ccgttgccca ccggcaatat agtcagccag
     1261 tgcaacacaa gcgtcatttc cggaatccac aattaaacca cggcttaaat cacgtaccga
     1321 tacgcgatcg ccctctttca aaaacatcag tgaagaaccg acaaacactg gattatcttt
     1381 cgcccacgca tcgcgcccca cggtgacaat atcgtctggc gtaatgcgat gactatcgat
     1441 agtgcgatcc acgacatagc ccgtcatcag ctttgtcagg ctggcgggat tgcgctgttg
     1501 atgctcatta cccgcggtga ggatctgacc ggtggtgtaa tccatcagta cccaggagcc
     1561 ggcatgaatc tctggaggct gaggtgaaaa aggaatgttt tccgccgcaa aaccagacga
     1621 taagttaaaa atgaacaaag aagcagcaat aataagacgg cgtttcaaca gcaaaccctc
     1681 aggagtttca aatagctgtt ctttttacgg aaatacttat gaactggctg gaataaagtg
     1741 caagaaaatg tgactaccct ctcattttta tctgacatga tctgttgtca ctcgctgtca
     1801 aattgtcgcg ctaaagctga ttagcacggt gatatttgat actctggcag acagcagaaa
     1861 taacggattt aacctaatga tgaatgacgg taagcaacaa tctacctttt tgtttcacga
     1921 ttacgaaacc tttggcacgc accccgcgtt agatcgccct gcacagttcg cagccattcg
     1981 caccgatgac gaattcaatg tcatcggcga acccgaagtc ttttactgca agcccgcgga
     2041 tgactattta ccccagcctg gagcagtatt aattaccggt attaccccgc aggagacacg
     2101 agcgaaagga gaaaacgaag ccgcgtttgc cgcccgtatt cactcgcttt ttaccgtacc
     2161 gaagacctgt attctgggct acaacaatgt gcgtttcgac gacgaagtca cacgcaacgt
     2221 tttttatcgt aatttctacg atccttacgc ctggagctgg cagcatgata actcgcgctg
     2281 ggatttactg gatgttatgc gtgcctgtta tgccctgcgc ccggaaggaa taaattggcc
     2341 tgaaaatgat gacggtctac cgagctttcg ccttgagcat ttaaccaaag cgaatggtat
     2401 tgaacatagc aacgcccacg atgcgatggc tgatgtgtac gccactattg cgatggcaaa
     2461 actggtaaaa acgcgtcagc cacgcctgtt tgattatctc tttacccatc gtaataaaca
     2521 caaactaatg gcgttgattg atgttcctca gatgaaaccc ctggttcacg tttccggaat
     2581 gtttggtgca tggcgcggca atacaagctg ggtggcaccg ctggcgtggc atcctgaaaa
     2641 tcgcaatgcc gtaattatgg tggatttggc aggagacatt tcgccattac tggagctgga
     2701 tagcgacaca ttgcgcgagc gtttatatac cgcaaaagcc gatcttggcg ataacgccgc
     2761 cgttccggtt aagctggtac atatcaataa atgtccggta ctggcccagg cgaatacgct
     2821 acgcccggaa gatgccgacc gactgggaat taatcgtcag cattgcctcg ataacctaaa
     2881 aattctgcgt gaaaatccgc aagtgcgtga aaaagtggta gcgatattcg tggaagccga
     2941 accgtttaca ccttcagata atgtggatgc acagctttat aacggctttt tcagtgacgc
     3001 cgatcgtgca gcaatgaaaa ttgtgctgga aaccgagccg cgtaatttac cggcactgga
     3061 tatcactttt gttgataaac gaattgaaaa gctgttgttc aattatcggg cgcgcaactt
     3121 cccggggacg ctggattatg ccgagcagca acgctggctg gagcaccgcc gccaggtctt
     3181 cacgccagag tttttacaag gctatgcaga agagattcag atgctggcgc agcagtatgc
     3241 cgacgataaa gaaaaagtgg cgctgttaaa agcgctttgg cagtacgcgg aagagattgt
     3301 ctaatgtgaa gcctccgccg ctggtacggc ggaggacaat tttgagtcag gctttttgaa
     3361 cggtgatgct ccacgctgca tcgccaatct gctgataatc ggtgatggca tgtccttctt
     3421 ctgccgccca ctgtgggatt gcttcagttg cctgggtgca atcaaactca attaccaact
     3481 catcgccgct ggccatttct gccagtgctg cttttgcttc tatgagtgga aacggacaga
     3541 cctgcgtcac cacatccagc tttttaatcg ccatctttac accttactta atttgccgca
     3601 gcagttgcca gtcgtgcctt acgctgcggt cgaacataca caagccagga cgcagtccag
     3661 actccgagaa tcataaatac aaggccaatc cagccctgcc aggtcatcat tgcagtcatg
     3721 accagtccat taccgataga gcaaccacct gcaatgctgg caccgaagcc catcagtaca
     3781 ccacctaacc cgctacgtaa tgttgtttgt gcatcagcag cgcgaacgcg gaactcacgg
     3841 ctcgctttgg ctgcaataaa tgaccccacg aagatcccta acaccaggaa aacgccccag
     3901 ttaatgtatt tcacgtcacc cgcgaccaga aattgcagaa tattggccgt tggggaagtg
     3961 attcctaacc caaacatgcg tccggttgct tcactcaggg gccaagctaa aagcgcaatc
     4021 aaaccgatga gtacagcagt gacaaaggga tgccagcgtt tttcaaacag aatatgagcg
     4081 atcccggttc ggcgcggcgg taaggtcgcg actttgagtt ttggcttctt caactctttc
     4141 atcaccaccc agagcgttat caccagcaaa acggcaacca acggccacac agacaaatta
     4201 aaagtatcag caatagagtt atgttcagta gtgtagtgct gcaaggtttg atttaaacca
     4261 ctggcgtgtg gagaacgcat caccgcactc atcaccatat aagtgaaaag cgcgatccaa
     4321 ctgccgatca atccctcacc cgcgcgatac caggtcccgg tggcacatcc acccgccaga
     4381 acaattccca gcccgaaaag atacccacct ataacagtac caagccacgg gaacgccccg
     4441 gcttcgtaag tcagtaggcc cgcctgaatt aacgcaaaaa cccccacgct ttgtaccgaa
     4501 attgcaatca gcaaggcgta aaacatgcga ttatttttcg cgatatacat atcgcgaaaa
     4561 ccacctgtca ggcaaaaacg cccacgctgc atgacaaatc ccagcagagc accacaaatt
     4621 agcccgctta atatcattga aaacataaat taattaacca gatgaatgtt aatggggaaa
     4681 ttattcatga ctggtggaat gcagaccaat aaccaaattc tctaaattag aacaaatggt
     4741 tattaatgag gatttcgata aaaaagaata aaaaaaccgg agccaaatgt tcattcgact
     4801 ccggtttatt attagaaaag atgattatgc tacgtcttcg tactgcggaa ccggattacg
     4861 gaaactcttg gttacgcaag ccaggtaaat cagaccgata gcagcccaga tcagacccag
     4921 aaccattgag ctttcttcca ggttaaccca cagcgcacca acggtcagcg caccacacat
     4981 cggcaggaac agatactgga agtgatcttt cagcgtcttg ttacgcttct cacggatcca
     5041 gaactgcgaa ataaccgaca ggttaacgaa ggtgaacgcc accagcgcac caaagttaat
     5101 cagcgccgta gccattacca ggtcgaagtt gattgccagc aaggcaatcg cgccaaccag
     5161 gatgatgttc attgccggag tacgccattt cgggtgtaca taaccgaaga agcttttcgg
     5221 gaacacaccg tcacgcccca ttacgtacat cagacgcgct acgcctgcat gcgccgccat
     5281 accggacgcc agtacggtaa tggtggagaa gatcagcgcg ccaacctgga acgctttacc
     5341 cgcaacgtac agcatgattt caggctgtga cgcatccgga tctttaaagc gagagatatc
     5401 cgggaagtac agctgcagga agtaagttgc aaagatgaag atcatgccgc caatcagtgc
     5461 ggtcaggaaa atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt
     5521 gctgatgccg tcaaagccgg taaaggagaa gcacaggatt gtcgccccgg taatcatcgg
     5581 gataacatgc gcatcgccag accagaacgg acgagtgctc gccagcgtac ccgcgccttc
     5641 accttcaaat acgccataaa caaccatgcc cagaatcacc gcgatcagca ctacctgcaa
     5701 cacgacgatt acagtgttga agttcgctac ggatttcaga ctacgcaggt taaaggcggt
     5761 cataaaggcc accagcgcca ccacaaacat ccacgatggg atggaaggca ccagagcttc
     5821 aaaatagatt ttcgccagca gaatgttgat catcggcgcg aacagatagt cgagcagaga
     5881 agaccaaccc accataaagc cgacagtcgg gctaatggat ttctgggcgt aagtgtatgc
     5941 agagccagca gaaggatagc ggcgaaccag cttcccgtag ctcagagccg taaacaggat
     6001 cgcaatcaat gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat
     6061 accaaatgta tcaaacagcg tcatcggctg catataggca agacccatca ttacaaccgg
     6121 aactaacgta agcgttttac gcaattccac gcgagaggtg tttggagtaa cgttatgcga
     6181 catggtcatt ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt
     6241 tggattcctc agcgacaaca actgtcgatt tttagtaaat atctatccgg tacgaagccc
     6301 ggcctcttgg tatgaatgat tggtttgaag caaaaaataa ccgacgctaa ttaaacgtcg
     6361 gtttttagtc attttttgac agcggcgcat tgtgcctaaa tgggggggaa atgacaagag
     6421 aatgagaggc ttgtcataat aatttttctt taaatggctg attttccgtc atcagatttt
     6481 gcataaacac cggcgatcgc actatttgct aaaatttcgt cccgccacca accactggca
     6541 agccccgcgg tctgttcatt ccagccaatc cagacgggtt caaagagggt ttgggcgacc
     6601 actttcttct cgattaacgc accactatcg agaaaacgtt gcgccagata acggggtaaa
     6661 tagccgcagc cgaggccgct aatttgcagc tccagcttgg ttttaaaatc gaaaacggta
     6721 atagcttctt gttcgtcgag aagttgtgag gccgtagaag cgccggcctg tgcagtgtcc
     6781 cccaccacaa tcgcccgata tcgtttaatg atacgacggt ttaacggttc ttcttccagg
     6841 gctaacggat gatgaggcgc gaccgcaaag acttgttcca gatcgcccaa ccgagagaaa
     6901 ccaaactcac tggaagatgg tggctcatgc atcgcgccaa caataatatc cgctcgcccc
     6961 tgagtcaggg catcccatga accaccgagt acaccattga taaacttcag gcgcgtcacg
     7021 ctatgatgtt gataaaaagc ctcaatgagt ggcgcaagca gagaaaaagg gaaggtgtcg
     7081 tccacgccaa tcaccagctc gttttcccag ccttcatgca gtttgatcgc ctgcttttcc
     7141 agttctcgca cggtatgcag aacttcccgc cctttctcta ataacatttt tccggtgcgg
     7201 gtgaatttag cgcggtgtcc gctgcgatca agcaattgaa tattaaggtc gctttccagc
     7261 ttgtgaacgg tgtaactcag agcggacggc gtcttgtaga gtttggccga cgccgccgca
     7321 aaactgcctt ctttttctaa ggcatcgagg atcatcaaca catccagcag tggtttcata
     7381 cacgccccct tgcagtgtat gcggcgtcct gctggcgtga tcgtttactc cagcggcatt
     7441 accagcggat cgggatactg gtattcaaat cccagttcat tacaaatccg actgccatca
     7501 ataatcttac ctttgccgct gtccagacta tttctgaact gcggaggttc cagccccaat
     7561 aaacgggcca tctgcggata gaaaacatta cgcgcagggt gagctggcgc acatatatta
     7621 tagatgtgtc cgcctttagg tgcctgtaac aacagagtga tagcgccaat aacatcttct
     7681 aaatggacta aattaacacc atgttcacca tcaggggcgg tttttccggc aaagaagcgt
     7741 ccgggatgac gtcccggtcc gaccaggccc gcaagacgca gaatatcgac cgaagtaccg
     7801 ggtaaattgt gcagccagtc ttcgagttct tctaatactc gtccactgtt ggtcaccgga
     7861 ttacgcgggg tggtttcttt caccgtgcct tgcgcgtcgc catagacaga tgtcgagctg
     7921 gtaaaaataa tgcggggaat acgatgggcc agcgcgctat ccactaactc ttgtaccgct
     7981 tgtaaataga actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat gaccagcgca
     8041 tcggcatcca tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg ctccatgcga
     8101 agcagatagc tatcaatgcc actcattcgg gccgcttcga caccatcttg tgtggtttta
     8161 ctcccggtga cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat gcctaaccac
     8221 cctaacccga caattgcgac ctttttcatc cgttatctcc tgatacacct tagatctata
     8281 aggctacgct agcgtatcaa aactgacaat tcattctatg aatgaatctg ttcaataatg
     8341 ataacgacat gctgcaatga gcagtgaatc atcagtaacc gcgtatacca gacggtgctc
     8401 ctctgtaatg cgtcgggacc agaaacctga caaattatgt ttcaggggtt ctggtttccc
     8461 cttaccttca aatggtgttc tgcgggtatc tttgataagt tcattgatct ttttaacaat
     8521 tcgcttatct gtttcctgcc agtacagata atcatcccat gattcctcag accagattag
     8581 tttcactcaa taatgtcctt ttccgttcct ttgcctgatt tcaggctatc gattgagtcc
     8641 atcaatctcc gggcgttagc gggggaacgc agtagataag ccgtctcttc cagcgagttg
     8701 tattcttcga gtgacatcag aacacaagcc tctccattct gacgagtaat gaggatcggg
     8761 gcatgatctt caacggcttt catcattgtt gccgacaaat tctgacgcgc ttcgctgtag
     8821 ctaattgtac gcatgtaaat ctcctctttt gtacagttca ttgtacaatg atgggtgtta
     8881 attcactatt tattaattag tttgtagatc aagacattgc cagcgagacg aaaaatcaga
     8941 ctttgctctt tttggtgctg tcaagttaga ggacagtcct cttagccccc tccttttccc
     9001 gctcattcat taaacaaatc catcgccata aaatatataa aaaagccctt gctttctaac
     9061 gtgaaagtgg tttaggttaa aagacatcag ttgaataaac attcacagag acttttatga
     9121 cacgcgttca atttaaacac catcatcacc atcatcctga ctagtctttc aggcgatgtg
     9181 tgctggaaga cattcagatc ttccagtggt gcatgaacgc atgagaaagc ccccggaaga
     9241 tcaccttccg ggggcttttt tattgcgcgg ttgttaacgg ttcagacagg tttaaagagg
     9301 aataagaaaa tgacagacaa cactcgttta cgcatagcta tgcagaaatc cggccgttta
     9361 agtgatgact cacgcgaatt actggcgcgc tgtggcatta aaatcaatct tcacacccag
     9421 cgcctgatcg cgatggcaga aaacatgccg attgatattc tgcgcgtgcg tgacgatgac
     9481 attcccggtc tggtaatgga cggcgtggta gaccttggga ttatcggcga aaacgtgctg
     9541 gaagaagagc tgcttaaccg ccgcgcccag ggtgaagatc cgcgctactt taccttgcgt
     9601 cgtctggatt tcggcggctg ccgcctttcg ctggcaacgc cggttgatga agcctgggac
     9661 ggtccgctct ccttaaatgg taaacgtatc gccacctctt atcctcacct gctcaagcgt
     9721 tatctcgacc agaaaggtat ctcttttaaa tcctgcttac tgaacggctc tgtcgaagtt
     9781 gcgccgcgtg ccggactggc ggatgcgatt tgcgatctgg tttccaccgg tgccacgctg
     9841 gaagctaacg gcctgcgcga agtcgaagtt atctaccgct cgaaagcctg cctgatccag
     9901 cgcgatggcg aaatggaaga atccaaacag caactgatcg acaaactgct gacccgtatt
     9961 cagggtgtga tccaggcgcg cgaatcaaaa tacatcatga tgcacgcacc gaccgaacgt
    10021 ctggatgaag tcatcgccct gctgccaggt gccgaacgcc caaccattct gccgctggcg
    10081 ggtgaccaac aacgcgtagc gatgcacatg gtcagtagcg aaaccctgtt ctgggaaacc
    10141 atggaaaaac tgaaagcgct gggtgccagt tcaatcctgg tcctgccgat tgagaagatg
    10201 atggagtgat cgccatgagc tttaacacaa tcattgactg gaatagctgt actgcggagc
    10261 aacaacgcca gctgttaatg cgcccggcga tctccgcttc tgaaagcatt acccgcaccg
    10321 ttaacgatat tctcgataac gtgaaagcac gcggcgatga cgccctgcgg gaatacagcg
    10381 cgaagtttga taaaaccacg gttaccgcgc tgaaggtgtc tgctgaggag atcgccgccg
    10441 ccagcgaacg ccttagcgac gagctaaaac aggcgatggc ggtggcagta aaaaatattg
    10501 aaaccttcca cactgcgcaa aaactgccgc cggtagatgt tgaaacacag ccaggcgtgc
    10561 gttgccagca agtcacgcgc ccggtagctt cagttgggtt gtatattcct ggcggctccg
    10621 ccccactctt ctcaacggta ttaatgctgg caactcctgc gcgtattgcg ggctgtaaaa
    10681 aagtggtgtt gtgctcaccg ccgccgattg ccgatgagat cctttatgcg gcgcagctgt
    10741 gcggtgtgca ggacgtgttt aacgtcggcg gcgcacaggc cattgccgct ctggcgtttg
    10801 gtacggaatc tgtgccgaaa gtggacaaaa tcttcgggcc gggtaacgcc tttgtcaccg
    10861 aagcgaaacg tcaggtgagc cagcgtcagg acggtgcggc gatcgatatg cccgcaggcc
    10921 cgtcggaagt gctggtgatt gctgacagcg gcgctacgcc agatttcgtg gcttctgatt
    10981 tgctttctca ggctgaacac ggcccggact cacaggtgat tttactgacg cccgctgctg
    11041 atatggcgcg tcgcgttgcc gaggccgtcg aacgccagtt ggcagaactg ccgcgtgccg
    11101 aaaccgcccg ccaggcactg aacgccagcc gcctaatcgt gaccaacgat ttagcacagt
    11161 gcgtggagat ctccaaccag tacggcccgg agcacctgat cattcagacc cgcaacgctc
    11221 gcgatctggt cgatagcatc accagcgccg gttcggtatt tcttggtgac tggtcaccgg
    11281 aatcggcagg tgattacgcc tccggcacca accacgttct gccgacttac ggttacaccg
    11341 ccacctgttc cagcctcggg ctggcagatt tccagaagcg catgaccgtg caggaactgt
    11401 cgaaagaagg cttttctgcg ctggcttcaa ccatagaaac actggccgcc gccgagcgcc
    11461 tgaccgccca taaaaatgcc gttactttgc gtgttaacgc ccttaaggag caagcatgag
    11521 caccgtgact attaccgatt tagcgcgtga aaacgtccgc aacctgacgc cgtatcagtc
    11581 ggcgcgtcgt ctgggcggta acggcgacgt ctggctgaac gccaacgaat atcccgcagc
    11641 cgtggagttt cagcttactc aacaaacgct caaccgctac ccggaatgcc agccgaaagc
    11701 ggtgattgaa aattacgcgc aatatgcagg cgtaaaaccg gagcaggtgc tggtcagccg
    11761 tggcgcggac gaaggtattg aactgctgat tcgcgctttt tgcgaaccgg gtaaagacgc
    11821 catcctctac tgcccgccaa cgtacggcat gtacagcgtc agcgccgaaa cgattggcgt
    11881 cgagtgtcgc acagtgccga cgcaggacaa ctggcaactg gacttacagg gcatttccga
    11941 caagctggac ggcgtaaaag tggtctatgt ttgcagcccc aacaacccga ccgggcaact
    12001 gatcaatccg caggattttc gcactctgct ggagctaacc cgcggaaaag ccattgtggt
    12061 tgccgatgaa gcctatatag agttttgtcc acaggcatcg ctggctggct ggctggcgga
    12121 atatccgcac ctggctattt tgcgcacact atcgaaagct tttgctctgg cggggcttcg
    12181 ttgcggattt acgctggcaa acgaagaagt catcaacctg ctgatgaaag tgatcgcccc
    12241 ctacccgctc tcgacgccgg ttgccgacat tgcggcccag gcgttaagcc cgcaggggat
    12301 cgtcgccatg cgcgaacggg tggcgcaaat tattgcagaa cgcgaatacc tgattgccgc
    12361 actgaaagag atcccctgcg tggagcaggt ttttgactct gaaaccaact acattctggc
    12421 gcgctttaaa gcctccagtg cagtgtttaa atctttgtgg gatcagggca ttatcttacg
    12481 tgatcagaat aaacaaccct ctttaagcgg ctgcctgcga attaccgtcg gaacccgtga
    12541 agaaagccag cgcgtcattg acgccttacg tgcggagcaa gtttaatgag tcagaagtat
    12601 ctttttatcg atcgcgatgg aaccctgatt agcgaaccgc cgagtgattt tcaggtggac
    12661 cgttttgaca aactcgcctt tgaaccgggc gtgatcccgg agctgctgaa gttgcaaaaa
    12721 gcgggctaca aactggtgat gatcactaat caggatggtc tgggaacaca aagtttcccg
    12781 caggcggatt tcgatggccc gcacaacctg atgatgcaga tcttcacctc gcaaggcgta
    12841 cagtttgatg aagtgctgat ttgtccgcac ctgcccgccg atgagtgcga ctgccgtaag
    12901 ccgaaagtaa aactggtgga gcgttatctc gctgagcaag cgatggatcg tgccaatagt
    12961 tatgtgattg gcgatcgcgc gaccgacatt caactggcgg aaaacatggg tattaatggt
    13021 ttacgctacg accgcgaaac cctgaactgg ccaatgattg gcgagcaact cactaaacga
    13081 gaccgttacg cccatgtagt gcgtaacacc aaagagacgc aaattgacgt ccaggtgtgg
    13141 ctggatcgcg aaggtgacag caagattaac accggcgttg gcttctttga tcacatgctg
    13201 gatcagatcg ccacccacgg cggtttccgt atggaaatca acgtcaaagg cgacctctat
    13261 atcgacgatc accacacagt cgaagatacc ggcctggcgc tgggtgaagc gttaaaaatt
    13321 gcccttggcg acaaacgcgg tatttgccgc tttggttttg tgctaccgat ggacgaatgc
    13381 cttgcccgct gcgcgctgga tatctctggt cgcccgcacc tggaatataa agccgagttt
    13441 acctaccagc gcgtgggcga tctcagcacc gaaatgatcg agcacttctt ccgttcgctc
    13501 tcttacacca tgggcgtgac gctacacctg aaaaccaaag gtaaaaacga tcatcaccgt
    13561 gtagagagcc tgttcaaagc ctttggtcgc accctgcgcc aggccatccg cgtggaaggc
    13621 gacaccctgc cctcgtcaaa aggagtgctg taatgaacgt ggtgatcctt gataccggct
    13681 gcgccaatct gaactcggtg aagtctgcca ttgcgcgcca cggttatgaa cccaaagtca
    13741 gccgtgaccc ggacgtcgtg ttgctggccg ataaactgtt tttacccggc gtaggcaccg
    13801 cacaagcagc gatggatcag gtgcgtgagc gcgaactgtt tgatctcatc aaagcctgta
    13861 cccaaccggt gctgggaatc tgcttaggga tgcaactgct ggggcggcgc agcgaagaga
    13921 gcaacggcgt cgacttgctg ggcatcattg acgaagacgt gccgaaaatg accgactttg
    13981 ctctgccact gccacatatg ggctggaacc gcgtttatcc gcaggcaggc aaccgcctgt
    14041 ttcagggaat tgaagacggc gcgtactttt actttgttca cagttacgca atgccggtca
    14101 atccgtggac catcgcccag tgtaattacg gcgaaccgtt caccgcggcg gtacaaaaag
    14161 ataacttcta cggcgtgcag ttccacccgg agcgttctgg tgccgctggc gctaagttgc
    14221 tgaaaaactt cctggagatg tgatgattat tccggcatta gatttaatcg acggcactgt
    14281 ggtgcgtctc catcagggcg attacggcaa acagcgcgat tatggtaacg acccgctgcc
    14341 gcgattgcag gattacgccg cgcagggtgc agaattgttg catctggtgg atctgaccgg
    14401 ggcaaaagat ccggctaaac gtcaaatccc gctgattaaa accctggtgg cgggcgttaa
    14461 cgtgccggtg caggttggcg gtggcgtgcg tagcgaagaa gacgtggcgg cgttactgga
    14521 agcaggcgtt gcgcgcgtgg tggttggctc caccgcggtg aaatcaccag agatggtgaa
    14581 aggctggttt gaacgtttcg gtgccgatgc cttagtgctg gcgctggatg tccgtattga
    14641 cgagcaaggc aacaagcagg tggcagtcag cggctggcaa gagaactcgg gcgtttcact
    14701 ggaacaactg gtggaaagct atctgcccgt cggcctgaaa catgtgctgt gtaccgatat
    14761 ctcgcgcgac ggcacgctgg caggttctaa cgtctcttta tatgaagaag tgtgcgccag
    14821 atatccgcag gtggcgtttc agtcctccgg cggtattggc gacattgatg atgtggcggc
    14881 cctgcgtggt actggcgtgc gcggcgtaat agttggtcgt gcattactgg aaggtaaatt
    14941 caccgtgaag gaggccatcg catgctggca aaacgcataa taccgtgtct cgacgtccgt
    15001 gatggtcagg tggtgaaagg cgtacagttt cgcaaccatg aaatcattgg cgatatcgtg
    15061 ccgctggcaa aacgctacgc tgaagaaggt gcagacgaac tggtgttcta cgatatcacc
    15121 gcttccagcg atggccgtgt ggtagataaa agctgggtat ctcgcgtggc ggaagtgatc
    15181 gacattccgt tctgcgtggc gggtgggatt aagtctctgg aagatgccgc gaaaattctt
    15241 tcctttggcg cggataaaat ttccatcaac tcccctgcgc tggcagaccc aacattaatt
    15301 actcgcctgg cggatcgttt tggcgtgcag tgtattgtgg tgggtattga tacctggtac
    15361 gacgctgaaa ccggtaaata tcatgtgaat caatataccg gcgatgaaag ccgcacccgc
    15421 gtcactcagt gggaaacact cgactgggta caggaagtgc aaaaacgcgg tgccggagaa
    15481 atcgtcctca acatgatgaa tcaggacggc gtgcgtaacg gttacgacct cgagcagctg
    15541 aaaaaagtgc gagaagtttg ccgcgtcccg ctgattgcct ccggtggcgc gggcaccatg
    15601 gaacacttcc tcgaagcctt ccgtgatgcc gacgttgacg gcgcgctggc ggcttccgta
    15661 ttccacaaac aaataatcaa tattggtgaa ttaaaagcgt acctggcaac acagggcgtg
    15721 gagatcagga tatgttaaca gaacaacaac gtcgcgaact ggactgggaa aaaaccgacg
    15781 gactgatgcc ggtgattgtg caacacgcgg tatctggcga agtgctaatg ctgggctata
    15841 tgaacccgga agccttagac aaaaccatcg aaagcggcaa agtcactttc ttctcgcgca
    15901 ctaaacagcg actgtggacc aaaggcgaaa cgtcgggcaa tttcctcaac gtagtaaata
    15961 ttgcgccgga ctgcgacaac gacacgctac tggtactggc gaatcccatc gggccaacct
    16021 gccacaaagg caccagcagc tgcttcggcg acaccgctca ccagtggctg ttcctgtatc
    16081 aactggaaca attgctcgcc gaacgcaaat ctgccgatcc ggaaacctct tacacagcca
    16141 aactgtatgc cagcggcacc aaacgcattg cacagaaagt gggcgaagag ggcgtggaaa
    16201 ccgcactagc agcaacggta catgaccgct ttgaactcac caacgaggcg tctgacttga
    16261 tgtatcacct gttggtgttg ttgcaggatc aggatctgga tttaacaacg gtgattgaga
    16321 acctgcgtaa acggcatcag tgagttgcgg ggtaagcgga tgcgatgttg ttgtcgcatc
    16381 cggcaaaaaa aacgggcaag gtgtcaccac cctgcccgtt ttctttaaaa ccaaaaagat
    16441 tacttcgcgt tgtaattacg cagagcgtta cgccccagca caatccccgc gccaaccatg
    16501 ccgcccagca gcaccgccag aatcaaagtg attgcctttt tcgggctatc gcgacgaata
    16561 ggtaacgtcg gtttcatcac atagcggtaa gcatgaatat caagatcatc aacttttaag
    16621 ctttcgatat ccagcaggtt ttgacgtgtc tgatagtagt ttggtgagaa caccaacgga
    16681 cgagtcgctt catgcttaat catcgactcc agcgcttcgc tccccaaaag gaacaacgta
    16741 tcttgtgtca catcttcagt ctgctgaacc tgtggctttg tcacctgcgc ctgattcgca
    16801 tactgcaacg cttcctgaat ctgacggata cgcagatctt tctgctcctg cgcaactact
    16861 tcctgagttt tcagagaatc ctgcaagttt ttcatacgca gcgcgatgtt gtctttcagg
    16921 tcttttccca gttcttcatt tacctggtcg tcaacttgct ggatatactt cgccagctgc
    16981 ttctgagcac cttcaggtgt ttcaccaaca taagacaccg ctaaaggtaa tgactgattt
    17041 ttgacggtag gctcaatggt cagcttttct gcttcttcct ggttatccag cgtttccgct
    17101 aatgctgaga atgcagtgct gtaacgtccg ataagtgaag cctgaatgtc ggagactttc
    17161 ggtacagccg atccgtagat cacattcatc gcgttggtat aaccagcgat ttgccccaca
    17221 tcaggttgag taacgatagc tgtggaagtc cacttttctt tggcaacggc cagataccct
    17281 atagccaggg caatagcaac aatgacagag atgataattg tcatcttgcc acgccacaac
    17341 tgcaccagta aatcaatcaa atcaatctgt tccgggtcat ggttttgccc agaaacatta
    17401 ttattttcta ctctcatcat taccctaacg gacgaaataa ttttaagtcg ccatagttta
    17461 gcgataatcg ccatgaatgc tataggataa atgataaaaa taatgaatta ttacaaaggg
    17521 aacataagag aatcagggcg aaaatcgccc tgataacagg atgttagtca ctgccaaaga
    17581 gatcgcgggt gtagactttg tctgccacat ccttaagctc ttctgccata cggttggaga
    17641 taattacgtc ggcttgttgt ttgaaagtgg cgagatcacg ttccagccga gagttgaaga
    17701 atgagtcttc tttcatcact ggctcgtaga tgattacttc aacacctttc gccttgatac
    17761 gcttcataat cccctgaata gaagacgcac ggaagttatc tgaaccgctc ttcataatca
    17821 ggcgataaat acccaccaca tgcggtttgc gtgacaaaat ggcatcggca ataaaatctt
    17881 tacgcgtgcg gtttgcatcg acaattgccg agatcaggtt attcggcaca gactgatagt
    17941 tcgccagtaa ctgcttggta tctttcggca gacaataacc accataacca aacgacggat
    18001 tgttgtaatg gttgccaata cgcggatcga gacaaacgcc ttcgataatc tggcgagtat
    18061 tcagacctaa actttctgcg tagctatcca gttcattaaa gtacgctacg cgcatcgcca
    18121 gataggtatt agcgaaaagt ttaatcgctt ctgcttcggt tgagtcggta aacagggtcg
    18181 ggatattttg cttaatcgcg ccttcctgta acagagcagc aaaacgttct gcgcgttctg
    18241 aacgctcacc gatgacaata cgtgaaggat ggagattatc gtaaagggct ttaccctcac
    18301 ggagaaattc cggagagaaa ataatatttt tagtacgata tttcttatgc atcgctgcgg
    18361 taaagccaac gggtaccgtt gatttgatga ccataaccgc ataaggattt atctctacta
    18421 cgtccttaat tactgattct acactcgatg tattgaaata attagtgtta ggatcatagt
    18481 cggttggagt ggcgatgatg acataatcag catcccggta ggcttcattt ttatctaatg
    18541 tggcattaaa gtgtatttta tctgattgca aaaactgctg aatttcctta tcaacaatag
    18601 gagatatccg atcattcagc atagcaacgc gtgacggtaa aatatctaat gccacaacct
    18661 catgattttg tgcgattaga agcccgtttg acaagcctac atagccggta ccggaaatgg
    18721 tgattttcat ttcatgctct cagaattaac ttaactgtga atcatgatgt ttttagcatc
    18781 ctgataagag ctaaaagttt taacgctacg ttgttcgatg gtcaacgcaa cgaaaaacaa
    18841 ttcagatttt ctttataaga atagctgata tttattaagt taatattaag cagtgaaatt
    18901 tagtctataa gataattggc aaaaaaaaag cccggcgtaa tatacgccgg gcctcaattt
    18961 attgttggtt aaatcagatt aatccagcca ttcagtatgg aacacacctt ctttatcaat
    19021 gcgcttataa gtatgcgcac caaaatagtc acgctgagcc tggatcaggt tcgcaggcag
    19081 aacagcggca cggtagctgt cataataggc aaccgcagcg gcgaaggtcg gaaccgggat
    19141 accgttctgt actgcataag caacgacatc acgcagcgcc tgctggtagt catcggcaat
    19201 ttgcttgaag tacggagcca acagcaggtt agcgatctga ggattttcgg cataagcatc
    19261 agttattttc tgcaggaact gcgcacggat gatgcagcca gcacggaaaa tcttcgcgat
    19321 ttcgccgtag ttcagatccc agttgtactc ttcagacgct gcgcgcagct gagagaagcc
    19381 ctgagcgtaa gaaacgattt tgcccagata cagcgcacgg cgaacttttt cgatgaactc
    19441 agccttgtcg ccagctggct gcgcttgcgg gccagagaga actttagatg cggcaacacg
    19501 ctgatctttc agagaagaga tataacgtgc aaacacagac tcggtaatca gcgacagcgg
    19561 ttcaccgaga tccagcgcgc tctggctggt ccatttaccg gtacctttgt tagccgcttc
    19621 atccaggatc acatcaacca ggtagttacc gtcttcatct tttttggtga agatgtcttt
    19681 ggtgatgtcg atcaggtagc tactcagttc accgttattc cactcggtaa aggtctgtgc
    19741 cagttcttcg ttagagagat tcaggccgcc tttcaggaga gaataggctt cagcaatcag
    19801 ttgcatatca ccgtattcaa taccgttgtg aaccatcttc acatagtgac ccgcgccatc
    19861 agcaccaata taggtaacgc acggttcgcc atcttcagca acggcggcga ttttagtcag
    19921 gatcggtgca accagttcat aggcttcttt ctgcccacca ggcataatgg aaggaccttt
    19981 cagtgcgcct tcttcaccac cggagacacc ggtaccaatg aagttaaaac cttctgcaga
    20041 aagctcacga ttacgacgaa tggtgtcctg gaagaaggta ttaccaccat caatgatgat
    20101 gtcaccttta tcgaggtatg gcttaaggga atcaatagca gcatccgtgc ctgcacctgc
    20161 tttcaccatt aacaggatgc gacgaggcgt ttccagagat tcaacaaact ctttcaccgt
    20221 atagtaagga accagtttct tgcctggatt ttcggcaatc acttcttccg tcttttcacg
    20281 ggaacggttg aaaatagaga cggtataacc acggctttcg atgttgagcg caaggttgcg
    20341 ccccatcact gccataccga ctacgccgat ctgttgcttt gacattgttt actcctgtca
    20401 ggggtggtca cgcggggtta cgcggcttga agaagctaag atggtaaccc agcatgtata
    20461 gaataagata gaagaataaa cggtggtgta tcgccctata accacttaga aaaagcctta
    20521 gctttcagtg ggtcaaatgg cccctccttt atctctaaca taagggcttt tggtgacagg
    20581 catttgacac tatgtatatc tcctggggaa aattccacga cacttattcc cgtaccgtct
    20641 ccaacaacaa actttttttg gagctcacca ttttgctcat acaaacaaac ttctaattgc
    20701 ccttgcatga cgacaaacat ttcccactga tgcggtaact catgccaatg gggttcaaca
    20761 tagctgtcgc gtacaaatgc aataagtaaa cgttgaacct tctcctgatg agatgcgtgc
    20821 aataaataat gagcgcgcaa acgttcagaa tttgctgcag tgcgaaaaag ttcatccata
    20881 atattgtcat caactaaacg cattctcctg cctccagtcg gacttcgata gtatgtgccg
    20941 ccgattcaac cgaaaattga gactttaaca acacattggc gttctgacct aactgttttc
    21001 ttaaaactga atcactaaga agcaattgtg cagattcaaa cagtttatca tcttcaccat
    21061 taacatgaat gaacccagcc ctgtgcttat taattacatc cattagatca ttaccgccat
    21121 tcacactccc aaggatcggg attgattgaa ccatataccc tagtaatttt ccggggaaat
    21181 tatgtgaaga atggcgagac gaaagggaga acaggccaac atcaacttca gataaaatta
    21241 atttaaactc ttcctggttc actgaaggta gatgagtgaa attagttaaa ttccattctg
    21301 cagaaagaga tttaatcagg tcaacttcat caccctgccc tataaacagg aaatgagcat
    21361 catgatgacg catcatatta cgcgcaaggc gcattaagtt tgccatatcc tgagcatgcc
    21421 caatattacc gccatagaaa aaaatgattt tatcttttag agcgtatttt tgacgaagtg
    21481 aatgataatc atcgccggca gacacaggag tcattgaggc ccaattacgt aaaacttcac
    21541 acggataacc tttattggcc tgacgaaata tctcaagatt cttatcagac attaacccaa
    21601 tccagtcagc ctgttgatat gatttttttt caaaatacct gaaatatttt tcaattggcg
    21661 agccggcttt tatcatacca gcatcaatga cccactgtgg gaacatatcc cttaggacca
    21721 gatagcttgg gcactggcat cgctctttaa tttttttaac caagcctccc caaaaaatag
    21781 agggggaata ataaacgata ccatcaaatg tatcatgttg gatgagatgc tttaatgcgt
    21841 gccatgcacg aaaagataaa agagtttcat ttatggcacg tttagcctta cctatatcct
    21901 ttaaaggtcc acttttgaaa cgccaaacct ttataccatc agtcatgcta acagaataga
    21961 ttgcctgtaa tgtgttgtca ggcgtaatta cagttacatc atggcctctg ctcagcaatt
    22021 ccaggcctaa ctcatgaaac attttagccc caactcgtgt gctatggggc aaataatcat
    22081 caatgattaa tgcaagtttc attagtattg cttccagaca acccgtttaa cgtagtcagt
    22141 atatgaatgg ataatacgca caactttatc tgaaacattt ggcatactat agtcactgac
    22201 ctgacgaagt aagcgtactt ctccacgagg ctgtgttgca ataatttcta atgcctgtaa
    22261 cacacgggca gactcaagac caaccatcat tacagcccct tcttcgaagc cttcagggcg
    22321 ttcgtgcgct tctcgtatat tgagcgcagg gaagttcata atggaggact cctctgtaat
    22381 agtcccgcta tccgataaaa cagcacgtgc atttttttgc agatggttgt aatcgtgaaa
    22441 tcctaatggc ttaagtagca agatattttt atgaaattga ataccgtttt cgttgatgcg
    22501 gttacgagtg cgcggatgag tagaaacaac taccgggata tcatattttt cagccacggt
    22561 attaagtata tttgccagtt tcgcaagctg tttaggggta tcaacatttt cttctctgtg
    22621 ggcacttacc acaaagaaat tcccaggtgt taaattcagg cgagaaagta catcggaacc
    22681 atcaatctgc gacatataat gagtgagtac ttcaaacatt gggctaccgg ttttaataat
    22741 cctatcggct ggtacacctt cagccaaaag atattcacgc gcgatatcac tatatgtcat
    22801 attgatatca gcggtgtggt caactatttt tctgttagtt tcttccggta cgcgttgatc
    22861 aaaacaacga ttcccagcct ccatatggaa aatcggaatt cttcgacgct ttgctggtat
    22921 tgctgaaata caggagttag tatcgccaag aactaacatg gcttctggtt tttcctgttc
    22981 aaagacctca tcaactttaa tgataacttg tccaatagtc tctgctgcat ttttacctgc
    23041 ggcattaaga aaataatcag gtttgcgaac acccaaatcc ttgaaaaaaa cctcattcaa
    23101 ttcataatcg taattttgcc cagtatgaac aataaggtga tcacaatatt catctaattt
    23161 tgcaaggaca cgcgagagtc gaataatttc tggacgagtc ccaacaaccg acatgacttt
    23221 caattttttc acgataaaac tctcgctata gtatctggtt cagaacgatt aaatatttca
    23281 tttgcccaaa gcataacaac tagctcatcc gagccattat tagtaatgtc atgcgtccat
    23341 cccggaactg tttcaacaat tttaaaatca tctgatgaaa cattaagttc atatcgttca
    23401 cccgtgacaa tattttcaaa tttgaaacaa gcacttcctc ggatgataat aaatttctca
    23461 tttttggaat gatgataatg ccccccccga gtaattcctg gatgcgcagt aaaaaacgaa
    23521 aactggcccg cgtttttcgt tttcaatact tcacagaata cccctctgtc atcactataa
    23581 gaaggaaccg tatacgcaaa ctgtttagga gaaaggtaac ttaaccatgt tgagtacaat
    23641 gctcgagtga atccattacc tacatcttcg atgattaatg tttggcgact ttctttaaat
    23701 cgataaatta attgtgccac ctcaccaaca gtaacagaat aaattggacc aaatgtcctg
    23761 taaccagttt cgttcgctcc ttctaatagc tttaatatgt cagaacaaaa gtcatctata
    23821 tacaccaaat ttacaactgc tgaaggatca ttaattgtaa tagcttcatc atttgcaatg
    23881 cgatggcaga aagttgctat aaaggagtta taatttggtt gacaccactt accgaataca
    23941 ttcggcaagc gataaatata atatttagcg tttgtcgttt ccccatactg ctgaatgatt
    24001 ttttccgcaa ctgctttact ctttccataa gcgttatcac attcagcttg gatggatgaa
    24061 ctaagcatga tag
//
LOCUS       DAZGOB010000068        24015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGOB010000068 DAZGOB010000000
VERSION     DAZGOB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.068"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..3865)
                     /gene="entF"
                     /locus_tag="ACHLIF_002897"
     CDS             complement(<1..3865)
                     /gene="entF"
                     /locus_tag="ACHLIF_002897"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO9606887.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIR"
     gene            complement(3862..4080)
                     /gene="ybdZ"
                     /locus_tag="ACHLIF_002898"
     CDS             complement(3862..4080)
                     /gene="ybdZ"
                     /locus_tag="ACHLIF_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO9606888.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4083..5284)
                     /gene="fes"
                     /locus_tag="ACHLIF_002899"
                     /pseudo
     CDS             complement(4083..5284)
                     /gene="fes"
                     /locus_tag="ACHLIF_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
     gene            5527..7767
                     /gene="fepA"
                     /locus_tag="ACHLIF_002900"
     CDS             5527..7767
                     /gene="fepA"
                     /locus_tag="ACHLIF_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO9606889.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDILVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGMSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7807..7905
                     /locus_tag="ACHLIF_002901"
                     /pseudo
     CDS             7807..7905
                     /locus_tag="ACHLIF_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7942..8562
                     /gene="entD"
                     /locus_tag="ACHLIF_002902"
     CDS             7942..8562
                     /gene="entD"
                     /locus_tag="ACHLIF_002902"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO9606890.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8684..8836)
                     /gene="hokE"
                     /locus_tag="ACHLIF_002903"
     CDS             complement(8684..8836)
                     /gene="hokE"
                     /locus_tag="ACHLIF_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO9606891.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9187..9339)
                     /gene="hokE"
                     /locus_tag="ACHLIF_002904"
     CDS             complement(9187..9339)
                     /gene="hokE"
                     /locus_tag="ACHLIF_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO9606892.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9308..9577
                     /locus_tag="ACHLIF_002905"
     CDS             9308..9577
                     /locus_tag="ACHLIF_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606893.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            9781..10899
                     /gene="ybdK"
                     /locus_tag="ACHLIF_002906"
     CDS             9781..10899
                     /gene="ybdK"
                     /locus_tag="ACHLIF_002906"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO9606894.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10965..11213
                     /gene="ybdJ"
                     /locus_tag="ACHLIF_002907"
     CDS             10965..11213
                     /gene="ybdJ"
                     /locus_tag="ACHLIF_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO9606895.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWQRWFSLAD"
     gene            11278..11646
                     /gene="ybdF"
                     /locus_tag="ACHLIF_002908"
     CDS             11278..11646
                     /gene="ybdF"
                     /locus_tag="ACHLIF_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO9606896.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11740..12393
                     /gene="nfsB"
                     /locus_tag="ACHLIF_002909"
     CDS             11740..12393
                     /gene="nfsB"
                     /locus_tag="ACHLIF_002909"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO9606897.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12501..13748
                     /gene="ybdG"
                     /locus_tag="ACHLIF_002910"
     CDS             12501..13748
                     /gene="ybdG"
                     /locus_tag="ACHLIF_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO9606898.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13816..15192)
                     /gene="pheP"
                     /locus_tag="ACHLIF_002911"
     CDS             complement(13816..15192)
                     /gene="pheP"
                     /locus_tag="ACHLIF_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO9606899.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15294..18437)
                     /gene="cusA"
                     /locus_tag="ACHLIF_002912"
     CDS             complement(15294..18437)
                     /gene="cusA"
                     /locus_tag="ACHLIF_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO9606900.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18449..19672)
                     /gene="cusB"
                     /locus_tag="ACHLIF_002913"
     CDS             complement(18449..19672)
                     /gene="cusB"
                     /locus_tag="ACHLIF_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO9606901.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19688..20020)
                     /gene="cusF"
                     /locus_tag="ACHLIF_002914"
     CDS             complement(19688..20020)
                     /gene="cusF"
                     /locus_tag="ACHLIF_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO9606902.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20178..21551)
                     /gene="cusC"
                     /locus_tag="ACHLIF_002915"
     CDS             complement(20178..21551)
                     /gene="cusC"
                     /locus_tag="ACHLIF_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO9606903.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQETRAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSIRLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            21708..22391
                     /gene="cusR"
                     /locus_tag="ACHLIF_002916"
     CDS             21708..22391
                     /gene="cusR"
                     /locus_tag="ACHLIF_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO9606904.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNRLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22381..23829
                     /gene="cusS"
                     /locus_tag="ACHLIF_002917"
     CDS             22381..23829
                     /gene="cusS"
                     /locus_tag="ACHLIF_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO9606905.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5790 a         6543 c         6110 g         5572 t
ORIGIN      
        1 cgcgaataat cggcccaatt tttacaaacg cccctggaga gataatatcc acatgcgcac
       61 aatcctgacg atagatatcc agctccgcaa tccacggcga ccaggcacgt tcgggactca
      121 taccttcctg aagtgtgcgc tcagcaacaa ataacgttgc ttttccatca aacggaacgc
      181 tatgggcggt ggttaacaag cgcacagcat cagcgtagtt gccttcaatg gtggtaaaca
      241 actccgttga agtacttccc tgctgtgccg ccaggaaggc ctcgcgttcg cggttaatct
      301 ccgccagcac ttccgggtcc agaccattag cctctttttc ctgccagttt tgcgtctctg
      361 gcggccaggt atccagcaaa ccaagaaatg ccacctgttc gccacgggca cgcagtcgcg
      421 ccgcaatacc ctgcgccagc gtaccgccaa gggaataccc cagcaggtaa taagggccgt
      481 gcggttgttg ctcaagtaac gttgccagat gcgcttcgca gacttcatcc aggtttgtcg
      541 ctgtctgcat gggtccatga gggcgcggcg actgaatgcc aataatcgac cattgtggat
      601 cgagataacg cgagagcacg ctaaactgcc aggcaaaacc ggacgccgga tggaaacaaa
      661 acagcgtcgg gccattacct tcacgcaacg gcagaatggt ttcgaatccc atgcgccggg
      721 agctgtcctc ttcaccatca ataatcgttg ccagtttggc gacggttgac gcgaccatca
      781 cctgccccgg cgtcacctga cgagcaaact gtcgacttaa ctgcgctgct agtttcattg
      841 ccagtagcga atgaccgccc agcgcaaaga aatcagcatc ggcatcctgc acgtcacaac
      901 ccagcaacga cgcgaatgcc gcggcgataa tcgtttcact gcccgctttc ggtgcacgtc
      961 ccgacgcttg tgtcttcagt tcaggcaacg gtaaggcttt gcgatccagc ttgccgttgg
     1021 cgctaagcgg caactgcgga agttgcagca gaacgaccgg caccatatgc ggcggcaatg
     1081 tttcgcgaag ctgtgcctgt aatgcgctgg tatccaacgg cagtcccgat tgcgacacca
     1141 gatagcccac caactgacgc gcatcaccac cggtggctgc cgcctggtta atcacacagg
     1201 cgtgggtaac tgcctgttcg acatccggca gcgcctgcat cacgcgatcg atttcgccca
     1261 gttcgatacg ctgcccgcga atttttagct gatcgtcact gcgcccgagg tactccaccg
     1321 cgccgttatc cagccagcgg gcgacgtctc cggtacgata catccgttca cctggggcaa
     1381 aaggatcggc aataaaacgg ctggcggtca gatcgggtcg tccaagatac ccctgcgcca
     1441 gttggatgcc ggtgagatag agatcacccg ccacacccgg cggcaccgga tgcatcatcg
     1501 catcaagaat acgcagaccc gtattccata ccggataacc aatcggcaca ctgctgccgc
     1561 gcacctgtgc cagttcctcg ccaaaagccg gataccagct gacatctacc gccgcttccg
     1621 tcgggccata caggttatgc aacggcgcgc cggttaactg ttgccattcg cggcataaat
     1681 cagccggtaa ggcctcacca ctacagaaaa cctgtttcaa cgtcgcgcaa ttctggcgag
     1741 cggtttgcgg cgtcagcgat gcaacaaatg ccgccagcat cgacggcaca aagtgcgtgg
     1801 tcgttacgcc atattcggca aagaattgct gcatagcgag cgggtcacgg tgcgcttccg
     1861 gttcagccat caccagtttt gcccctgcga taaacggcca gaaaaactcc cacaccgaga
     1921 catcaaaact gcacggcgtt ttttgggcaa cgacatcttc gcctgtaagc gggtagtgat
     1981 tttgcatcca taacaggcgg ttaacgatag ccgtctgccc gaccattacc cctttcggcc
     2041 tgccggtgga gccagaggta aagatgatat aagccgtgtg gtgcggttgt gaaagttgca
     2101 gcggcgcact gccctggggg gtaagcgggg cgttatagca aaggcttgtt aaattgggaa
     2161 catcgctaaa gcgcggcagt tgatcgtcgg tggtaattaa cagcgacgga cgcgcatctt
     2221 ccagcatcat tttcaggcga tcgtccggat agccggtatc cagcggtagc caggccgcgc
     2281 cagcttcaac tattgcatgg agtgccaggg tcaaaaagac cgagcgcggt agtgccaccg
     2341 ccacgctgtc ccctggttta acgccgcgct cacgcagcag attcgccagc gccaccacct
     2401 gctcgcgcat ttcccgatag ctgaacaggt aacgcgcatc tgccagcgcc ggagcatccg
     2461 gtgtttttgc cgcttgttct gccaccagcg cgctcagcgt ggtttctgga atctcaacct
     2521 gagtggcgtt gatctgcgcc agctgcgcat actcacctgg cagcataata tcgacatcgc
     2581 cgcacaacag cgccggatcc gcggcgaact gggcaatcag cattttcagg cgttcagcat
     2641 gctggattaa cgttggctca tcgtaacgct gtttattggc gaggatctca atactcaaat
     2701 caccgtgttc atccgggaac agggccagtt caaggtcatt aaccggaccg gttgccaggg
     2761 tatgggtttg cgtctgaaca ccaggaatat ccagttggta atcaaatacc ttgatattga
     2821 gtaccggacc gaacagcggt tcctctcctg ccgctcgccc gctgtcacgg acaatttgtt
     2881 cggcatcgta acgttgatga cgacgcattt ttttcagttg tgctgccagt cgggttgcca
     2941 gttccggcag cgtttcttgc gccgcaatgt gaatacccaa cggtaaaacg ttgagcacgg
     3001 gtccgctagc cgtcagcgcc gccgagccca gtcgacgcat aaagataaat ccggcggcat
     3061 agtccatgcg attgcacaat cgccccagcc acaaggctgc cagcgcaagg gctaaatcgg
     3121 tacgctgcac acctgaaagt tgcgtagcca gctggcggaa ttccccgtcg gtaaattcca
     3181 gtttcaggcg cagaatatct gccgaagcgc tgcgccccgc taaaggtgcc ggagaaagtg
     3241 acgcgggcgg cggcagttga cgacgctgtt ctgcccagaa tgccgcatca cgctgccagg
     3301 cttcgctttc gcggtattgc tggtactctt ccactacatc agcgaaaggc gtaaacggcg
     3361 aagcaggcgt tggttcgcca cgcagccatg tgcagtaaat attggcgatc tggcgagtaa
     3421 tggccgggaa actgaagcca tcgaccagca aatggtgata acgctgatac cagtaccagc
     3481 ggttatccgc aacatgtatc agctgatgaa agaccagtgg tttaccgcta tcgacgcgca
     3541 aatcttgttg caaatccgcc agcattaatg cctgcgcagt accgtgcgga tcaatattgg
     3601 ttcgcaggtc gataatttct ggcagttcga atatcagcgc atcatcgacc cactgccaga
     3661 cttcgccgtt atcttccgta aaacgcatcc gcaacgtatc tgcttgcgct agtccgacaa
     3721 ccaccgcgcg ggccagtaat ggcgcatcaa catctccagt taactcaacg taatgcgcca
     3781 cgctccaggc ggagggtaaa tccgacagtt tttctgccat ccagatgccg ggctgtgcgg
     3841 cgaccaaagg taaatgctgg ctcattgtgc ctcctgcaac tgggtgaaat tggccggtgt
     3901 cagagtacgc cagtggactt ccagccactg ctggcaggac gcctgtgact gcggctgaca
     3961 cacaatgtcc cagcctgccg gtaaggcgca ttgttgcggc cacagactga attgcccctg
     4021 cgcattgcgc aaaatgtaaa acgctccctg tggatcatcg aagggattac tgaatgccat
     4081 attcaactcc tgtcatggaa agtggttgcc agaggtcgat tagcccctgc atcaagccac
     4141 cgcgccaaca aagcgcatca tgtccgccgt caacctgacg ccagaaaatg gattctttta
     4201 tggggtgtaa ttgcgcatac agcgcctgat tggcccgcat aatcatcggc tcgcgaatac
     4261 ccgcttccag cacaatgcgc agaccttcgg cgctaacttc accagcttta agcttttcaa
     4321 gtaacacgcc ctcttgctgc ccgccccgat gcggccacca gtacgatcct gactggctta
     4381 atacacagcc aaagcgttca ggccagtgca gtccggcata cagcgcggac agcccaccaa
     4441 aactctgccc ggcgaccacg gtgcgatcgg cgcgatcgct aaaaggggca atagctttca
     4501 ccaggggtaa taactcttgc tgtactgcca gccagaaatc cgcattacac ggcagttcgt
     4561 gggcgcggtg cgtggtgtcg atagcgtcga tcaacacata cacggcggga ggaagttgct
     4621 gacgatgggt cagcgaagtc agcacaggcc agacgggcat actttgcgcc caaaattcgc
     4681 catcgagcaa aactgccagc ggacgttctt ctgctgttgc atcgccggtg gtaaaaatcc
     4741 atacacgacg tgaatttttc aaccgttcac ttttccagat aatttctttg gctgctattt
     4801 ctggcgcttg cggacaatcc catcccggtt gcagaggcgc ttgcggcatt tcgagtgcag
     4861 aaacagcgtg ccctcgcccg cctttccagc tttgtggatt cagcggatcg gctattgcct
     4921 ggggtaatag ttttcgccag ccttcacgta attcgaggcg atcgggggat ggtgcagaaa
     4981 aaatgtcatc gcgttcggtg ggaataaagc agtagctgcc gcgccagttg gcattgagtt
     5041 gtgtcgtcca ctgccagaca tcagtacctg caattcgctg catcgactgg ggctggctgt
     5101 tctgatgatg atcggtcaca ccagtgatgt agatccatac gcgctttatc gtcgagtatt
     5161 cttcagaacc ttggggatca cgccaccaga aagtgacctc aaacatttcg tcattcagac
     5221 gctgccattc cgggccatgt ttcgactgcc accagctctc acttcctact tttaacgccg
     5281 tcacaccata accccatgtt tactgtgcaa tttttcattg attgcagaaa tatattgata
     5341 atattattga taactatttg catttgcaat agcgtaatgg cgcgccgtgg gaagcgcgga
     5401 catttattaa ccaactgcac tgcgtgtctt tcaggatcaa aggttttcgc ggtagcggga
     5461 tgcgtcgtgt tgaggacgac catgcccgac agttgcaatt cgtggcaaaa atgcaggaat
     5521 aaaacaatga acaagaagat tcattccctg gccttgttgg tcaatctggg gatttatggg
     5581 gtagcgcagg cacaagagcc gaccgatact cctgtttcac atgacgatac cattgtcgtt
     5641 accgccgccg agcagaactt acaggcgcct ggcgtttcga ccatcaccgc agatgaaatc
     5701 cgcaaaaacc ccgttgcccg cgatgtgtcg gagatcatcc gtaccatgcc aggcgttaac
     5761 ctgaccggta actccaccag tggtcagcgt gggaataacc gacagattga tattcgcggt
     5821 atgggtccgg aaaacacgct gattttgatt gacggcaagc cggtaagcag ccgtaactcg
     5881 gtgcgtcagg gctggcgtgg cgagcgcgat acccgtggtg atacctcctg ggtgccacct
     5941 gaaatgattg aacgtattga agttctgcgt ggtccggcag ctgcgcgtta tggcaacggc
     6001 gcggcgggcg gcgtggttaa catcattacc aaaaaaggca gcggtgagtg gcacggctcc
     6061 tgggacgctt atttcaatgc accagaacat aaagaggaag gagccactaa acgcaccaac
     6121 ttcagcctga ccggtccgct gggcgacgaa ttcagcttcc gcttgtatgg caacctcgac
     6181 aaaacccagg ctgacgcgtg ggatatcaac cagggccatc agtccgcgcg tgccggaacg
     6241 tatgccacga cgttaccagc cgggcgcgaa ggggtgatca ataaagatat taacggcgtg
     6301 gtgcgctggg acttcgcacc attgcaatcg ctggaactgg aagcgggtta cagccgccag
     6361 ggtaacctgt atgcgggcga tacccagaac accaactctg acgcttacac tcgctcgaaa
     6421 tatggcgatg aaaccaaccg cctgtatcgc cagaactacg cgctgacctg gaacggtggc
     6481 tgggataacg gcgtgaccac cagcaactgg gtgcagtatg aacacacccg taactcgcgt
     6541 attccggaag gtctggcggg cggtaccgaa gggaaattta acgaaaaagc gacacaggat
     6601 ttcgtcgata tcgatctcga tgacgtgatg ctgcacagcg aagttaacct gccgattgat
     6661 atcctcgtta accagacgct gacgctgggt acggagtgga atcagcaacg gatgaaggac
     6721 ttgagttcca atacccaggc gctgaccggg gcgaacaccg gcggcgctat tgatggtatg
     6781 agtgccaccg accgtagccc gtattcaaaa gcagaaattt tctcgctgtt tgccgaaaac
     6841 aacatggagc tgactgacag caccatcgta acgccggggc tgcgtttcga tcatcacagt
     6901 attgtcggca ataactggag cccggcgctg aacatatcgc aaggtttagg cgatgacttc
     6961 acgctcaaaa tgggcattgc ccgtgcttat aaagcaccga gcctgtacca gactaacccg
     7021 aactacattc tctacagtaa aggtcagggc tgctatgcca gcgcgggcgg ctgctatctg
     7081 caaggtaacg atgacctgaa agcagaaacc agcatcaaca aagagattgg tctggagttc
     7141 aaacgcgacg gctggctggc gggcgtcacc tggttccgta acgattatcg caataagatt
     7201 gaagcgggct atgtggctgt agggcaaaac gcagtcggca ccgatctcta tcagtgggat
     7261 aacgtgccga aagcagtggt tgaaggtctg gaaggatcgt taaacgtacc agttagcgaa
     7321 acggtgatgt ggaccaataa catcacttat atgctgaaga gtgaaaacaa aaccacgggc
     7381 gaccgtttgt cgatcatccc ggagtacacg ttgaactcaa cgctgagctg gcaggcgcgg
     7441 gaagatttgt cgatgcaaac gaccttcacc tggtacggta agcagcagcc gaaaaaatac
     7501 aactacaaag gtcagccagc ggttggcccg gaaaccaaag aaatcagtcc ttacagcatt
     7561 gtgggtctga gcgcgacctg ggatgtgacg aagaatgtca gtctgaccgg cggcgtggac
     7621 aacctgttcg acaaacgttt gtggcgtgcg ggtaatgccc agaccacggg cgatctggca
     7681 ggggccaact atatcgcggg tgccggggcg tatacctata acgagccggg acgtacgtgg
     7741 tacatgagcg taaacactca cttctgatgc taacgtcaga ttgttgacaa agtgcgcgtc
     7801 gttcatgccg gatgcggcgt gaacgcctta tccggcctac aaaaatcttg ccaattcaat
     7861 atattgcagg accatgtagg cctgataagc gtagcgcatc aggcagttta gcgtttgtca
     7921 tcagtctcta atatggtcga catgaaaact acgcatacct ccctcccctt tgccggacat
     7981 acgctgcatt ttgttgagtt cgatccggcg aatttttgtg agcaggattt actctggctg
     8041 ccgcattacg cgcaactgca acacgctgga cggaagcgca aagccgaaca tttagccggg
     8101 cggatcgctg caatttatgc gctgcgggaa tatggctata aatgtgtgcc cgcaatcggc
     8161 gagctacgcc aaccggtgtg gcctgcggag gtatacggca gtattagcca ctgcggggct
     8221 acggcattgg ccgtggtttc ccgtcaaccg attggcgttg atatcgaaga aattttttct
     8281 gcgcaaacag cgaccgaatt gacagacaac attattacac cagcagaaca cgagcgactc
     8341 gcagactgcg gtttagcctt ttctctggcg ctgacactgg cattttccgc caaagagagc
     8401 gcatttaagg caagtgagat ccaaactgat gcaggttttc tggactatca gataattagc
     8461 tggaataaac agcaggtcat cattcatcgt gagaatgaga tgtttgctgt gcactggcag
     8521 ataaaagaaa aaatagtcat aactctgtgc caacacgatt aattgacaac atctggtacg
     8581 attcgcccgc agccatcact gaccgcgggc gaaagtgtaa agcaggtgcc ttaccactct
     8641 gacctgacaa ccggatatgc ggggattgct ccccgcacaa cggctacttc ttcggttcgt
     8701 aagcgagaac agccttaaac tcaatattac gttcctttac ggtgaactca cacagcgagt
     8761 ctccgaccag aagcgtaaat cccagtaccg tcaaacacag cactattact gccacaaggg
     8821 catatttcgt cagcatgttt atattgcctc catgcgaaga agggactacc atccgagttg
     8881 ttgaggctcg gagtggcagc cccaggattg atagaaatat ctcctggggc ttcatctttc
     8941 cagacctcag agttaacctg aaaccggaaa gcttcaggca cccgccgtta tcttaccctt
     9001 cctcaacaaa atatctgttt atttatacag tgaatcctgt ttcggacttc tgtcggcaaa
     9061 actcatcatt gctcttaaaa cgaaatcgcc cgcagccatc actgaccgcg ggcgaaagtg
     9121 taaagcaggt gccttaccat cctgacctga caaccggata tgcggggatt gctccccgca
     9181 caacggctac ttcttcggtt cgtaagcgag aacagcctta aactcaatat tacgttcctt
     9241 tacggtgaac tcacacagcg aatccccgac cagaagcgta aatcccagca ccgttaaaca
     9301 cagaactatg actgccgcaa gggcatattt cgtcagcatg tttatattgc ctccatgcga
     9361 agaagggact accatccaaa ttgttcaggt ttgggtaggt agccccaggg ttgatagaaa
     9421 tatcgtcctg ggacttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca
     9481 ggcacccgca gttatcttac ccttgcttag caaaatagct gtttatttat acagtaagtc
     9541 ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt tttttctgca
     9601 tgcctcaatc gtcaaaattc ttcgccacca gccagccgtg ccataacttg tcacattgca
     9661 aaagtagcca aattgcttca gtcaccatca ccaaaaaaag aaacattcca gaacttccgc
     9721 atctcgccca gccgctccga cttctatact gaatagaaaa cgccaactta agagaaacct
     9781 atgccattac ccgattttca tgtttctgaa ccttttaccc tcggtattga actggaaatg
     9841 caggtggtta atccgccggg ctatgactta agccaggact cttcaatgct gattgacgcg
     9901 gttaaaaatg agatcacggc cggagaggta aagcacgata tcaccgaaag tatgctggag
     9961 ctggcgacgg atgtttgccg tgatatcaac caggctgccg ggcagttttc agcgatgcag
    10021 aaagtcgtat tgcaggcagc cgcagaccat catctggaaa tttgcggcgg tggcacgcac
    10081 ccgtttcaga aatggcagcg tcaggaggta tgcgacaacg aacgctatca acgaacgctg
    10141 gaaaactttg gctatctcat ccagcaggcg accgtttttg gtcagcatgt ccatgttggc
    10201 tgcgccagtg gcgatgacgc catttatttg ctgcacggcc tgtcacggtt tgtgccgcac
    10261 ttcatcgccc tttccgccgc gtcgccgtat atgcagggaa cggatacgcg ttttgcctcc
    10321 tcacgaccga atattttttc cgcctttccc gataatggcc cgatgccgtg ggtcagtaac
    10381 tggcaacaat ttgaagccct gtttcgctgc ctgagttaca ccacgatgat cgacagcatt
    10441 aaagatctgc actgggatat tcgccccagt cctcattttg gcacggtgga ggttcgggtg
    10501 atggataccc cgttaaccct tagccacgcg gtaaatatgg cgggattaat tcaggccacc
    10561 gcccactggt tactgacgga acgcccgttt aaacatcagg agaaagatta cctgctgtat
    10621 aaattcaacc gtttccaggc ctgccgctat gggctggaag gcgtcattac cgatccgcac
    10681 actggcgatc gtcgaccgct aacggaagat accttgcgat tgctggaaaa aatcgcccct
    10741 tccgcacata aaatgggtgc atcgagcgcg attgaggccc tgcatcgcca ggtcgtcagc
    10801 ggtctgaatg aagcgcagct gatgcgcgat ttcgtcgccg atggtggctc gctgattggg
    10861 ctggtgaaaa agcattgtga gatctgggcc ggtgactaag tagggaattg ccttacagaa
    10921 gaatatccct gacaatggtg ttttaactta catttaacaa tgttatgaaa caccctttag
    10981 aaaccttgac taccgcagca ggcattttgc tgatggcttt cctctcttgc ctgctgctgc
    11041 ccgcccccgc actggggctg acgctggcac aaaaactggt gaccatgttc catctgatgg
    11101 atcttagcca gctttacact ttattgtttt gtctgtggtt tttagtgctg ggcgctattg
    11161 agtattttgt tctgcgcttt atctggcaac gctggttctc gctggcggat taaacgtcga
    11221 ttatcgccgt acttcgcggc atactttgct tattctcctt cgcctaaagg aacgtttatg
    11281 gataagcaat cactgcacga aacggcgaaa cgcctagccc ttgagttacc ctttgtcgag
    11341 ctttgctggc cttttggccc ggagttcgat gtttttaaaa ttggcggcaa aatttttatg
    11401 ctgtcgtcgg agctgcgcgg cgtccccttt atcaatctga agtccgatcc acaaaaatcc
    11461 ctgttaaatc agcagatata cccgagcatt aagccagggt atcacatgaa taaaaagcac
    11521 tggatttcgg tgtatcccgg cgaggaaatc tccgaagcgt tacttcgcga tctgatcaac
    11581 gattcgtgga atctggtggt tgatggtctg gctaaacgcg atcaaaaaag agtgcgtcca
    11641 ggctaaagcg gaaatctata gcgcattttt ctcgcttacc atttctcgtt gaaccttgta
    11701 atctgctggc acgcaaaatt actttcacat ggagtcttta tggatatcat ttctgtcgcc
    11761 ttaaagcgtc attccactaa ggcatttgat gccagtaaaa aacttacccc ggaacaggcc
    11821 gagcagatca aaactctcct gcaatacagc ccatccagca ccaactccca gccgtggcat
    11881 tttattgttg ccagcacgga agaaggtaaa gcgcgtgttg ccaaatccgc tgccggtaat
    11941 tatgtgttca acgaacgtaa aatacttgat gcctcgcacg tcgtggtgtt ctgtgcaaaa
    12001 accgcgatgg acgatgcctg gctgaagctg gttgttgacc aggaagatgc tgatggccgc
    12061 tttgccacgc cggaagcgaa agccgcgaac gataaaggtc gcaagttctt cgctgatatg
    12121 caccgtaaag atctgcatga tgatgcagag tggatggcaa aacaggttta tctcaacgtc
    12181 ggtaacttcc tgctcggcgt ggcggcgctg ggtctggacg cggtacccat cgaaggtttt
    12241 gacgccgcca tcctcgatgc agaatttggt ctgaaagaga aaggctacac cagtctggtg
    12301 gttgttccgg taggtcatca cagcgttgaa gattttaacg ctacgctgcc gaaatctcgt
    12361 ctgccgcaaa acatcacctt aaccgaagtg taattctctc ttgccgggca tctgcccggc
    12421 tatttcccct cagattctcc tgatttgcat aaccctgttt cagccgtcat cataggctgc
    12481 tgttgtataa aggagacgtt atgcaggatt taatatccca ggttgaagat ttagcgggta
    12541 ttgagatcga tcacaccacc tcgatggtga tgattttcgg tattattttt ctgaccgccg
    12601 tcgtggtgca tattattttg cattgggtgg tactgcggac cttcgaaaaa cgtgccatcg
    12661 ccagttcacg gctttggttg caaatcatta cccagaataa actcttccac cgtttagctt
    12721 ttaccctgca ggggattatc gtcaatattc aggcggtatt ctggctgcaa aaaggcaccg
    12781 aagcggcaga tattctgact acctgcgcgc agttgtggat catgatgtat gcgctgcttt
    12841 cagtcttctc gttgctggat gttattttga atctggcgca gaaattcccg gcggcatctc
    12901 agttaccgct gaaagggata tttcagggga ttaaactgat cggcgcaatc ctggtcggca
    12961 ttttgatgat ctcgctgctg attggtcagt cgccagcgat tctgatcagc ggtcttggtg
    13021 caatggctgc cgtgctgatg ttggttttta aagatccgat tcttggtctg gtggcaggta
    13081 ttcagctttc cgcgaacgat atgctgaaac tgggcgactg gctggagatg ccgaaatacg
    13141 gcgcggatgg cgcggtgatc gatattgggt taaccaccgt caaagtgcgt aactgggaca
    13201 ataccattac cactattccc acctggtctc tggtttctga ctcctttaaa aactggagcg
    13261 ggatgtcagc atctggcggg cgacgtatta agcgcagtat cagtattgat gtcaccagta
    13321 ttcgttttct tgatgaagac gaaatgcaac gtctgaataa agcgcacttg ttaaaacctt
    13381 atttaaccag ccgccatcag gaaattaatg agtggaatcg ccagcaaggt tctacggagt
    13441 cgatattaaa cctgcgccga atgaccaata ttggaacctt tcgtgcctat ctgaacgaat
    13501 atctgcgtaa ccatccgcgg attcgtaaag atatgacctt aatggtacgc caactggctc
    13561 caggtgataa cggtttaccg ctcgagatct acgcgtttac taacaccgtg gtgtggctgg
    13621 aatatgaaag cattcaggct gatatattcg atcacatatt tgcgattgtc gaagagtttg
    13681 gtctgcgact tcatcagtcg ccaaccggca atgatattcg ctctctggcg ggtgcattta
    13741 agcagtaatt aaaaaaaccg ctctcatcga atggatgaga gcggacatcg ttttgtcgga
    13801 tgtagcgtga atgccttatt tccgacgcag cgttttaaat gccacaaaca ggaatacaat
    13861 ccacaccggc agcaggatcg ctgacaagcg catatcatcc atcgtgcaca tcagcagcaa
    13921 aatcatgccg aggaaggcaa tgcaaagata gttgccgaac ggatagagca gcgccttaaa
    13981 ctgtgtttca cgcccctgac gtcgcatcgc tgcacgaaaa cgcagatgcg ccagacagat
    14041 cataatccag ttcaacagca gcgttgctac caccagcgcc atcagcagac caaacgcttt
    14101 ttgcggcagc agatagttga ttaataccac cagcgaagtg atcgctccgg aaagcatcag
    14161 cgagttaatc ggcacaccgc gacggctgac gcgagtcaaa aactttggcg cattaccctg
    14221 cacagaaagg ccaaacagca tgcggctgtt agagtaaacc ccgctgttat acactgacag
    14281 cgatgctacc agaatgacga agttcagcgc agaagctacc acgttgctgt cgagattatg
    14341 gaaaatcatc acaaacgggc tactgttgga tttcacttcc acccacggat agagcgccag
    14401 taaaaccacc agtgaaccga tgtaaaacag caggatgcga tacaccacct gatttaccgc
    14461 ttttggaatg cttttttccg gatcgcgcgc ttcagcggca gtaatcccaa tcagctccag
    14521 accgccgaag gagaacataa ttaccgccag cgacaaaatc agcccattcc agccggtggc
    14581 aaagaaacca ccgtagcgcc agaggttgtc gatactggct ttctcgccgc cgtgaccaga
    14641 aaacagcagc cacaggccaa agccgatcat accgatgatc gccagcactt taatcagcgc
    14701 aaaccagaac tcggtttcac catataagcg cacgttcacc aggttaacgg cgttgatgat
    14761 aataaagaag gcggcagccc aaatccacgt tggaacatcc gggaaccagt actgcatata
    14821 gatgcccgca gcggtcagct ctgccattcc caccagcacg aacattaccc agtagttcca
    14881 gccagagagg aagcccgcaa acggtcccca gtatttatag gcaaagtggg caaatgaacc
    14941 ggataccggc tcttcgacca ccatctcgcc aagctggcgc ataatcagga aagcgatgat
    15001 cccggcgacg ccgtagccca gcaatacagc cggacccgcc atctgaatcg ccgggccaat
    15061 gccaagaaac agaccagtac caattgcgcc acccagcgca atcagttgaa tatgacggtt
    15121 atgtaatccg cgatgaagcg tcggctcttg attcgacgca gtatcttccg atacggttga
    15181 cgcgtttttc acgcctttcc cctgtgtgtc ttttttgttg aggggcacct tttaacattt
    15241 agtgcccatc gtcgcaagac acaatccaca cggttaaacg gggtatcctg cttttatttc
    15301 cgtacccgat gtcggtgcag ccacatcagc ttatacgccg ccgggataat aaacagcgac
    15361 agcaaaggtg cggtgatcat gccgccaatc atcggtgcgg caatccggct catcacctct
    15421 gaaccagccc ccgttcccca cagaatcggc agcagaccgg cgataatcac cgccaccgtc
    15481 atcgctttcg ggcgcacgcg caggaccgcg ccgtgatata acgcctcatc cagcttctgc
    15541 tcgctgaatg tttgtggatt attcaacgac ggctcggcct ctatggcgtg acgtaaatac
    15601 atcagcatca ccacgccaaa ttcggcggcg accccggcga gggcgataaa gccagtgccc
    15661 gtcgccacgg aaagatgaaa gcccatccac cacagcaacc agatgccgcc caccagcgca
    15721 aacggtacgc tgctgataat cagcaacgct tcgcccaccc gacggaacgc cagatacaac
    15781 agcacgaaga taatcatcaa cgtcatcggc accatgagtt taagcttatg gttggcgcgc
    15841 tccagtagct cgaactgccc ggagaatgcc acgctggtgc ccggttttaa ctgcactttt
    15901 tcagctatcg ctttttgcaa atcgtgaacc accgacacca tgtcacgatc gcgggcatcg
    15961 atataaatcc agctcgtcgg gcgcgcattc tcggttttca gcattgacgg tccggtagag
    16021 actttaatgt cggccacgtc tgccagggtg atttgctgct tcatcggcgt caggatcggc
    16081 aactggcgca gtgcctgcgg actatcgcgc cagctttgcg gataacgcag attaattgga
    16141 taacgggcaa tcccttccac cgtttcgcca accatcgccc cgcccaccgc agaagtcaca
    16201 aacaactgca catccgccac cgtcataccg taacgtgcgg ctttttcacg gttaatctca
    16261 acgttgatat agcgcccacc ttccagacgc tcggctagag cagaagccac gcctggcacc
    16321 gttcgcgcta cttcttcaat ttgctcagcc atcgcgtcga tatccgccag cacagtgccg
    16381 gaaactttaa tgccgatggg gcttttaatg ccggttgaga gcatatcgat acggttacga
    16441 attggcggca cccacagatt cgccagcccc ggcagacgca cggtgttatc cagttcctca
    16501 atgattttgt ccattgtcat gcctggccgc cactgctcct gcggcttaag ctggatggtc
    16561 gtttctacca tctccagcgg agcagaatcg gtggcggttt ccgctttccc ggttttgcca
    16621 aatacccgcg ccacttcagg tacgctcata attagcttgt cggttttttg cagcatactc
    16681 gccgcctctg ctgcggaaat ccccggcagc gtcgatggca tatacaacag gtcgccttca
    16741 ttgatctgcg gtaaaaattc cccgccaact ttattgagcg gccagagaac cgtcagcacc
    16801 gaaagcgctg ccaccaacag cgtggttttc ggccagtgca gtactttcag caacagcgga
    16861 tgataaacac gaatcaaaaa gcgattgagc gggttactgc tttccggcgg aattttgcca
    16921 cggatccagt aacccatcag gatagggatc actacgatcg ccagcagcgc cgcacccgcc
    16981 atcgcatacg ttttggtgaa cgccagcggg ccaaacagac gcccttcctg cccttccagg
    17041 gtgaagatcg ggataaacga caacgtgata atcagcagac tgataaatag cgccggaccc
    17101 acttcaacag atgcatcggt gatcacctgc cagcgcgttt tattatccag cgtggcgtca
    17161 ggatgctggt gctgccactc ttccagccgt ttatgtgcat tttcgatcat gacgatagcg
    17221 gcatcgacca tcgccccgac ggcaatcgca atgccgccca acgacataat attggcattc
    17281 agtccctgga agtgcatgac aataaaagca atacacaacc ccagcggcaa cgaaataatc
    17341 gccaccagcg ccgagcgcac atgccagaga aacagcgcac agactaccgc caccacaata
    17401 aactcttcca gcaacttgcc gctgaggttg tcgatggcgc ggtcaatgag ctggctgcga
    17461 tcgtatgttg taactatctc cacgccttcc ggcagactac ttttcagcgt ttccagtttg
    17521 tccttcacgg cggcgatcac ttctcgggcg tttttgccgg atcgcaggat caccaccccg
    17581 cccgccactt cgccttcgcc gtttagttcg gcaatgcccc ggcgcatctc cgggccaatc
    17641 tggaccttcg caacatcgcg cagataaacg ggcacgccat tttcactggc ttttaaaacg
    17701 atgtgattaa agtcgtcgag cgtttgcaga tagccgctgg cgcgcaccat atattccgct
    17761 tccgccagtt cgatcgacga accgcccgct tcctggtttg aagcatccag cgcgcttttt
    17821 acttcggcga gactgatgcc atactgcgcc aggcgctggg gatcgataac cacctgatac
    17881 tctttcacca caccgcccac cgacgccact tccgcaacgt cagggatggt tttcagctca
    17941 tatttgagaa accagtcctg caatgagcgt aaatcggcca gatcgtgctt accgctgcga
    18001 tccaccagtg catattcata gatccagcca acacccgtgg catctggtcc cagctcggcg
    18061 ctgactcccg caggcagctt accctgtacc tggttgaggt actccagcac ccgcgaacgc
    18121 gcccagtacg gatcggtgcc atcttcgaaa atgacataca cataagagtc gccaaactgt
    18181 gagaaaccgc gcacagtctt cgcgccaggc accgacaaca tggtggtggt tagcggataa
    18241 gtcacctgat tttcaacgat ttgtggtgcc tgaccgggat agctggtttt aataataacc
    18301 tgcacatcgg agagatccgg cagcgcatcc actggcgtat taatgatggt ccaggtgccc
    18361 cagatgctca gaaacaacgc gcccatcagc accaaaaaac ggttcgccac cgagcgacga
    18421 ataatccatt caatcattgg ttattccctc aatgcgcatg ggtagcactt tcagagcgca
    18481 tccgctccag tgcgccagaa atattggctt cggaatcaat caggaacagg ccgctggaaa
    18541 ccaccttttc accttccgcc agaccagagc gtaatgcggt gacgccttgc gatgcctgga
    18601 aaacagcaac gcgtttcggt acaaagcgcc cgtcggcatc aacggtaatc acccgctgtt
    18661 cgctgccggt atcaatcagc gcttgtgacg gaatgagcag catcggttcg ctggcggtgt
    18721 tgagttgcaa ccaggcgttc atgcccggtt ttagcgcctc gtcggcgttg tcgacttcca
    18781 gacgcagctg cagcgtgcgg gtcgcggcat ccacgccagg tagcagcgtc catttgcgga
    18841 tggtgagtgt tttatccggt cgcgccggaa cggtgagggt aaactgcgag gcatctttca
    18901 ccagccaggc gatggactcc gggatcgcag cagtgaccca caccgggtcc ataccctgaa
    18961 ttttcgcgac cacgttatct ttggcgatat tcattcccgc gcgcagatca aacgcggtga
    19021 tcacgccatc aatgggcgct ttgagcgtaa agcgagtctg gattttttgc gtggcgatca
    19081 ggcggcgaat atccgcctcc ggcattcccg ccagtcgcag ccgctcaaga atgccttcag
    19141 tctgggtcgc cgtaccgccg gtttcgcgca gcagtaaata ctcactctgc gcttccaccc
    19201 agtcaggaat ggtcaggtcg agaagcggtg tgcccttttg tactttatcg cccacggtaa
    19261 gcggatacac cttgtcgata aacccggcag cgcgagcctg cacaatggca tactgatact
    19321 cgttgtaact gacattcgcc gggaaactct gggcaaaagt cagcggcccg cgcgtgacgg
    19381 tagccgtttt cacccccaga ttctgcgtct gagtcgggtc aatgcgcaca ccagacgcag
    19441 aactctcttc atcggcatat ttcggcacca gatccatatc cataaacggc gatttacctg
    19501 gtttatcgaa ccgcgtattg gggtacatcg ggtcgtacca gaataagatt ttacgttctg
    19561 cggtcgacgt tttttctgcg ggtggttcag cctttgcaaa ccaggtaaaa cctgccgcag
    19621 aaataatacc gcccgcgatc atgctgccga taataagcgc gatttttttc atctcattaa
    19681 acctgggtta ctggctgact ttaatatcct gtaataaaga aaggttgccc tgctggacaa
    19741 aattaaacgc cactttgtcg ccagttttaa tttcactcat tttcgtctgc ggagtgatgg
    19801 taaagcgcat ggtcatctcc ggccagttca cggcagcaat cggatcgtga tggatggtga
    19861 tttttttgct ttccagatca atacccttta ccacgccagt ggcgctaata acctgcggtt
    19921 gtacttcgct catggtttca tgatgatgtt cgttagcctg ggcattaaag ccaataacgg
    19981 taaacagact gaacattgcg acttgcagtg cttttttcat ttattctctc ctggagttaa
    20041 aagtcatact ctaaataatt cgagttgcag gaaggcgaca agcgagtgaa tccccaggag
    20101 cttacataag taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta
    20161 tgacgagtat attgctgtca accgccacct aaggcggtat acaaagaaat ttcgttaacc
    20221 tgacgggcat aattcaggtc gagtaaagtt tgtcgggttg caaataaaga acgctcggca
    20281 tccagcactt ccagataact tactgcgccg tgctgatata atgcccgcgc ccgttgcaaa
    20341 gtaatttgca gcgacgccag ataacgctgc tgggcgctga tttgatcgtt caggctttga
    20401 cgtaatgcca gcgcatctgc cacttcttta aaggcgttct ggattttctg ttcataattc
    20461 actaccgact gctgctggcg aatttcggcg atatccagat tggcctggtt gcgtccggca
    20521 ttaaaaatgg ggatctcaat tttaggaata aaattccaca tcccgctgct ggcgttaaat
    20581 aatgacgaca gatcgctgct ggcggttgat attccgctgg tcagccttat agatgggaaa
    20641 aatgccgcgc gtgcggcccc aatattggca ttagccgcca ttaacgcgtg ttcagcttcc
    20701 ataatatccg ggcgctgcaa taagatttgc gacgacaatc ccgccggtaa tttaacgctt
    20761 tgcaggctgt cgctgtttac tgtctgcgct tgcggcagct tgccgtagct tcccaataac
    20821 agttgcaatg cattattcgc ctgcgccagt tccccctgac gtttagcgat gtcgctgcgg
    20881 gtactttcaa tcaccccgcg agcctgttcc agcgccagaa cattactgct acctgtcaac
    20941 agttgttttt cgacaaacgc atatgactgc tgataattac gcagcgtttc ttcggctatt
    21001 tgcaattgcg catacgctag ctgctgattg aaataacttt gcgcgacatt agaaaccagc
    21061 aaaatatgca ccgcgcgctg ggcttcctca gtggctaaat aattttggcg ctcggcttcg
    21121 ctcatgttct ttaagcgacc gaaaaaatcg agatcaaagc tggcgttaag gccggtcgaa
    21181 aactcccgtg tcgtggcggt attcccttta agattaccgc tccagctgcc gctgccctcg
    21241 ccattgagct gtgggtagcg gtcggcatcg gtcagacgat attgcgcccg cgtttcctgc
    21301 actttcagcg tcgccatgcg caaatcccgg ttattcacca gcgcctcgct aatcagcgtc
    21361 tttacctgat tatcaacaaa aaaggtgcgc cagcccacgt tctggtagtt atctgctgcg
    21421 ttaaccaagc cgttctggct gagtgagaac tgctgcggca cgggcattgc cggacgctga
    21481 taatccggtg ccagtgaaca accggttagc gcaagggcca cacaaaatgg cagaagttta
    21541 caaggagaca taggctcata atttctggtg attttatacc gccaacttta ctcgccaggc
    21601 tctgattttc cggtgacagg aaaatgacaa aattgtcatt ttgccaataa gcgattgcca
    21661 tctgatcccg ctactctaga attgcccggg caacatgcgg aggaaaaatg aaactgttga
    21721 ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa agggttaacc gaagccggtt
    21781 ttgtggtcga tttggccgac aacaggctga atggctacca tctggcgatg accggtgatt
    21841 atgatctgat aatcctcgat attatgctgc cggacgtgaa cggctgggat atcgtgcgca
    21901 tgttgcgttc cgccaataaa gggatgccga ttctgttgct taccgcgctt ggcaccattg
    21961 aacatcgcgt caaggggctg gagttggggg cagatgacta cctggtgaaa ccattcgctt
    22021 ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg cggggcggcg gtgattatcg
    22081 aaagtcagtt tcaggttgcc gatctgatgg tcgatctcgt cagccgcaaa gtcacccgca
    22141 gcggcacgcg catcactctg accagtaaag agtttactct gctggagttt ttcctccgcc
    22201 atcagggcga agtgctgccc cgctcgctta tcgcctcgca ggtgtgggac atgaatttcg
    22261 acagcgacac taacgccatt gatgtagcgg tgaagcggct gcgcggcaaa atcgacaacg
    22321 actttgagcc aaagctaatt cagaccgtgc gcggcgtggg ttacatgctt gaggtgccgg
    22381 atggtcagta agccatttca gcgcccgttt tcgctggcaa cccgcctgac cttttttatc
    22441 agcctggcca ccatcgcagc gtttttcgcc tttgcatgga tcatgatcca ctcagtaaaa
    22501 gtgcattttg ccgagcagga tattaatgat ttaaaagaaa ttagcgccac gcttgaacgg
    22561 gtacttaatc atcctgacga gacgcaagcc cgacgcttaa tgacgctgga agatatcgtc
    22621 agtggttatt ccaacgtgtt gatctccctg gcagatagcc acggcaaaac ggtgtatcac
    22681 tcccccggtg cgcctgatat ccgcgagttt gcacgtgatg ccatacccga taaagacgcg
    22741 cggggtggcg aggtgtatct cctttccggc ccgacgatta tgatgccagg ccacggtcac
    22801 gggcatatgg aacacagcaa ctggcggatg attaacttgt cggttggccc gttggtggac
    22861 ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg attttcatct tcattacata
    22921 aatgatttga tgaataaact tattatgacc gcatcggtaa tcagcatcct gatcgtcttt
    22981 atcgtactgt tggcggtaca taaaggtcac gcgccgatcc gcagcgtcag ccgtcaaatc
    23041 cagaatatca cctcgaaaga tctcgacgtt cgccttgacc cgcagaccgt gcccattgag
    23101 ctggaacagc tggtactgtc gttcaaccat atgatcgagc gtattgagga tgtctttacc
    23161 cgccagtcca atttctcagc ggatatcgct cacgaaattc gcacgccgat taccaatctc
    23221 atcacgcaaa cggaaatcgc cctcagccag tctcgcagcc agaaggagct ggaagatgtg
    23281 ctctactcta atctcgaaga gctgacgcga atggcgaaaa tggtcagcga tatgctgttt
    23341 ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga aaatgctcaa cctggcggat
    23401 gaagtcggca aagtgttcga ttttttcgag gcgttagcgg aagatcgcgg cgtggagttg
    23461 cgatttgttg gcgacgaatg tcaggttgcg ggcgatccgc tgatgctgcg tcgggcgtta
    23521 agcaacctgc tttctaatgc cctgcgttat acgccgcccg gagaggcaat tgtagtgcgc
    23581 tgccagacgg tcgatcacca ggtgcaagtt accgtcgaaa accccggtac gcccattgcg
    23641 cccgagcact taccgcgatt gtttgaccgt ttctaccgcg ttgacccttc ccgccagcga
    23701 aaaggcgaag gtagcggcat tgggctggcg atagtgaaat cgattgttgt cgcgcataaa
    23761 ggcacggttg cagtaacgtc agatgcgcgg gggacaaggt ttgtgattat gctgccggag
    23821 agagagtgat tattaagttc aaattcaggg cgtggtgcat cacctcctgg tattgttgat
    23881 caacaaatta gagaaaaact tattaaacat atgaaatatc gaaggctttt atttttcaaa
    23941 gtttcacgag atgtttgtat atgaaactta gttaaatctg tgtttcttcc aattgtagat
    24001 cacaatgccc tttct
//
LOCUS       DAZGOB010000069        23640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGOB010000069 DAZGOB010000000
VERSION     DAZGOB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.069"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            71..787
                     /locus_tag="ACHLIF_002918"
     CDS             71..787
                     /locus_tag="ACHLIF_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9606906.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            817..1317
                     /locus_tag="ACHLIF_002919"
     CDS             817..1317
                     /locus_tag="ACHLIF_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO9606907.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQITKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1409..4093
                     /locus_tag="ACHLIF_002920"
     CDS             1409..4093
                     /locus_tag="ACHLIF_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO9606908.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADISDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDGLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4090..5164
                     /locus_tag="ACHLIF_002921"
                     /pseudo
     CDS             4090..5164
                     /locus_tag="ACHLIF_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
     gene            complement(5252..6550)
                     /gene="exuT"
                     /locus_tag="ACHLIF_002922"
     CDS             complement(5252..6550)
                     /gene="exuT"
                     /locus_tag="ACHLIF_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO9606909.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7033..8445
                     /gene="uxaC"
                     /locus_tag="ACHLIF_002923"
     CDS             7033..8445
                     /gene="uxaC"
                     /locus_tag="ACHLIF_002923"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO9606910.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8460..9947
                     /gene="uxaA"
                     /locus_tag="ACHLIF_002924"
     CDS             8460..9947
                     /gene="uxaA"
                     /locus_tag="ACHLIF_002924"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO9606911.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10030..10581
                     /gene="ygjV"
                     /locus_tag="ACHLIF_002925"
     CDS             10030..10581
                     /gene="ygjV"
                     /locus_tag="ACHLIF_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO9606912.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10586..11830)
                     /gene="sstT"
                     /locus_tag="ACHLIF_002926"
     CDS             complement(10586..11830)
                     /gene="sstT"
                     /locus_tag="ACHLIF_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO9606913.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12229..13194)
                     /gene="alx"
                     /locus_tag="ACHLIF_002927"
     CDS             complement(12229..13194)
                     /gene="alx"
                     /locus_tag="ACHLIF_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO9606914.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13477..14463)
                     /gene="ygjR"
                     /locus_tag="ACHLIF_002928"
     CDS             complement(13477..14463)
                     /gene="ygjR"
                     /locus_tag="ACHLIF_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9606915.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14542..15221)
                     /gene="ygjQ"
                     /locus_tag="ACHLIF_002929"
                     /pseudo
     CDS             complement(14542..15221)
                     /gene="ygjQ"
                     /locus_tag="ACHLIF_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
     gene            complement(15298..15801)
                     /gene="ygjP"
                     /locus_tag="ACHLIF_002930"
     CDS             complement(15298..15801)
                     /gene="ygjP"
                     /locus_tag="ACHLIF_002930"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO9606916.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTPDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15886..17022
                     /gene="rlmG"
                     /locus_tag="ACHLIF_002931"
     CDS             15886..17022
                     /gene="rlmG"
                     /locus_tag="ACHLIF_002931"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO9606917.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSISDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17213..17296
                     /locus_tag="ACHLIF_002932"
                     /pseudo
     CDS             17213..17296
                     /locus_tag="ACHLIF_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17293..17709
                     /gene="higA"
                     /locus_tag="ACHLIF_002933"
     CDS             17293..17709
                     /gene="higA"
                     /locus_tag="ACHLIF_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO9606918.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17754..19772)
                     /gene="fadH"
                     /locus_tag="ACHLIF_002934"
     CDS             complement(17754..19772)
                     /gene="fadH"
                     /locus_tag="ACHLIF_002934"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO9606919.1"
                     /translation="MSYPLLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVASSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHHVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20098..22449)
                     /gene="ygjK"
                     /locus_tag="ACHLIF_002935"
                     /pseudo
     CDS             complement(20098..22449)
                     /gene="ygjK"
                     /locus_tag="ACHLIF_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(22466..23536)
                     /gene="ygjJ"
                     /locus_tag="ACHLIF_002936"
     CDS             complement(22466..23536)
                     /gene="ygjJ"
                     /locus_tag="ACHLIF_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO9606920.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
BASE COUNT         5955 a         6013 c         6092 g         5580 t
ORIGIN      
        1 ttgacttaca ataaatctac atctctaata caccaacttt ttggaaacaa tattgtaaga
       61 tcaaatttat atggtggcga aaattaatct cttatcacgg cttacgccgc ttttattcgt
      121 gttcgctcct tttttggcac agtcaaatat gactgtttat cctatggctg tttccattaa
      181 tagtcagggt gaaggcaacg ttcgcgtaat atctaaaagt aatgaagttc agtacatcaa
      241 ggcgacagta ttccgtatcg ataatccatc gacgcctcag gaaaatgaag ttgagattaa
      301 atccggtgat gcaaatcatc tggttgttat gccacctaaa ttcgctttac cagccggtag
      361 tagcaaaacc gtacgttttg ttgcgatgga accagagcaa aaagagaaaa attatcgcgt
      421 taaatttgaa gcggttccca gtattgatga cgttgccaca gataaaaaag atctctctat
      481 gcagttaaca gttaacttaa tttgggggat tgttgttagt gttccacctc agcaacctat
      541 tgctaagtta gaagtaaatg ctgcccaaaa attagttaat gcagggaatc aacgcttaaa
      601 gattttaacg atcgcttatt gtaaaaataa tagcaaagaa aattgtaaga tacagaccgt
      661 aaataaaaac atcttccctg gtcaggaaag aaatcttgaa agcatatctg gctacgacaa
      721 aatcgttgtc aaatataata actggatcac caaagataat ggcgagtttg aactggcggt
      781 ccattaatta tcattgtaat aaacgaagga atagtaatga aaaaggtatt tgcaaaatct
      841 cttctggtcg cagcgatgtt ttctgttgca ggctccgcat tggcagtaca aaaggatatt
      901 actgtaacgg ccaacgttga tgccgctctg gatatgacac agaccgataa caccgcgttg
      961 ccgaaagcag tagaaatgca atatctgccg gggcagggtc tgcaatctta ccagttgatg
     1021 accaaaatct ggtctaacga cgttaccaaa gatgtaaaaa tgcagttagt ttccccggcg
     1081 caactggttc aaagcctgga tgctagcaag attgttccgc tgacagtaac ctggggcggt
     1141 gaagaaatta aagctgatgc ggcaaccact tttaccgcaa ccaaaatctt tgcttccgat
     1201 gcgttaacta acggttctct ggctaaaaac ctgatgtttg ctcaaatcac caaaggtgtt
     1261 ctggagacag gcatctaccg tggtgtagta agcatttatc tgtctcagga tatctaatcc
     1321 agataacaga attaacacgt aacaaaggga ggggtagccc tcccttattt taactgtttt
     1381 agtatttgtt atggatttca ggattgccat ggataaaaaa ttactggctc ttttgatcct
     1441 ggcgagtctc agcccggcag aggcgacatt aaccaaaatt cccgcagggt ttgaggttat
     1501 tgctcaggga cagcaggagt atatcgaggt ttatttttca gggaaaagtc tcggtaaata
     1561 ttatgcaatg gttaatcttg ataccgtaac atttcttgat ccagcaagtc tatataacaa
     1621 gctggaactg gatgtagacg atcagaaaat cgcgcatata gtgaaagaaa aattatcgca
     1681 gccgctagct cgccacggtg aattggcttg cggttatgta cgtactgact cagggtgtgg
     1741 ttttctgaat accgatacgc tggaaataat ctataatgat gaagaaagtt cggcaacgtt
     1801 gtttattaat ccgcaatgga attcagcttt cgatgcgaag tcattatatt taaatccaga
     1861 caaaaatact gttaatgctt ttatacatca gcaagacatc aatgttctgg cacaggatga
     1921 ttaccaatcg ttgtctattc agggaaacgg tgcgctggga ataacagaaa atagttatat
     1981 tggtgcacac tggaatttca acggttatga tgcagatgat gtcagtgaca gtaatgctga
     2041 catcagcgat ctctattatc gttatgattt tttacgtcgt tattatgtgc aggcgggtcg
     2101 catggacaac cgcacactat ttaatgcaca aggcgggaac tttaccttta actttctgcc
     2161 actcggtgca attgatggga tgcgtatcgg gtcgacactg agctatttaa accaggcgca
     2221 aagccagcaa ggaaccccag taatggttct gctttcgcgc aattctcgtg ttgacgctta
     2281 tcgtaatgag caacttttgg gatcgtttta tctcaatagt ggttcgcaat ttattgatac
     2341 cagttccttt ccgccaggca gctacagcgt ggcattaaaa gtctacgaaa ataaccaact
     2401 cacccgcacc gagcttgtgc cgtttaccaa aaccggcggt ctgactgacg gaaatgcgca
     2461 atggttctta caggcaggta aaactacatc acaggtttct gatgatgaaa gttcagctta
     2521 tcagctagga gtacgcctgc cattacatcc gcaatatgag ctctacgcag ggctggcgaa
     2581 tgccgatgat gtgagtgctt tcgagttagg taataactgg acggcagatt taggcggggt
     2641 ggggaatctt gcaatcagcg ccagcgtgtt ccgtaacgat gacggcggca aaggtgatat
     2701 gcaacaggcc aactggagta atccgggatg gccgacgttg ggcttttatc ggaccaactc
     2761 tgacggcgat gcttgtacaa ccgacagcag agagagctat aacgccttaa gctgttatga
     2821 aagtatttcc gcgacggttt cacagaattt tgtcggctgg aatatgatgc tgggttatac
     2881 ccgcacacaa aataacactg atgatagttt gcgttgggat aaacagcaga gctttgaaaa
     2941 taactatctt cgccagacaa cagcgcaaag tatctctgaa actgtacaac ttagcgcttc
     3001 ccgcgctttt gtgatgcgtg actggatttt gagtacgtca gtcggtgttt tccatcgtaa
     3061 tgacaacggt ggcgataacg acgacaacgg cttgtactta tcgttttcgt tatctgacac
     3121 gccaacgatg gatagcaata acaacagcca ttcaacaaat gtgtctacgg attatcgtta
     3181 tagcgaacag gatggcgatc aaacgtcatg gcagttatcg catacctttt ataacgattc
     3241 attcagccat aaagaacttg gcgtaaccgt cggaggcctg aacaccgata ccataaacag
     3301 cgcggttaac gggcgttggg atgggcaata cggaaatgtc tacgctaccg tatctgatag
     3361 ttatgaccgt aagaatcatg atcatctttc ggcctttacg ggcacttata gctctacgct
     3421 ggctgtcagt cgctatggcg ttaatttggg agccagtggt acagacggtt tgctgggtgc
     3481 ggtactggtg gatgtgaaag gcttctctga acaggatgaa gagagtcagg atctgcaact
     3541 cgaagcgcgg gtggcaggca gccgaacttt gcagcttggt caaagtgaca gtgtgttgtt
     3601 cccttatcct ggatttcagt ctggttttgt tgaggttaac gacagtagcc agggcaatca
     3661 gcaggggaca acaaacatca ttaacggtgc agggaatcgt gaattaatgt tgttgcctgg
     3721 caagctgcgc tatcgcgaag tgtctgccag ctttaactac aactatatcg gtcgtttatt
     3781 actgccggca gcggtgaaaa aattcccgat tgtggggctg aacagcgcca tgttactggt
     3841 agctgaagat ggcggattta cacttgaaat taacggcagc gaaaaagagc tctatctgct
     3901 ttccgggcag caattcctta agtgtccact gagtgttgta aagaaacgcg ccagcattcg
     3961 ttacagcgga gatgttactt gtagtgtggt gacttattca caattaccgg agtcgattca
     4021 ggttcaggca cagttgaaac agcctaaatt acgtggaaac gttcagacgg cgcaaaggga
     4081 ggttgcacca tgagaaatcg attgattgcg gcgatattgg gcttgtttgg ttcaggcagc
     4141 tcctgacgtg accagtgaaa ttacgtatga tttggcatct ggtagagcgg attattactt
     4201 ctggaaggat gaagcgtcgg caggaaataa tggatatatg tggtatgaat gttcgtatcc
     4261 tgacctccaa caaacctgta cagctaatgg aaatatatcg acagtacaaa tctatttaac
     4321 tgaacaacgc agtgggatgc gttggccggt aaaactcaaa ggatttaaaa cagccattgt
     4381 aagtagcgat gaagcgccgc caggatgcaa ggggggcaaa gggcttcaga cgaatcttaa
     4441 ggattctaat agatcttcat gtacagaaga tggtcaacat tattatatat acgatacaaa
     4501 gtttcttacg ctctaccttg agcagacaga gatgaagaat ttgccgattg gtggcgtctg
     4561 gaaggggaaa gttaaattac attcgaacag cccggcccag gactatttcg caaatattac
     4621 cctgaatacg ctcgacccca accatattga cgtgttcttc ccggagttcg cccacgccac
     4681 gccaagggtg cagttagact tgcatccaac aggaagcgtt aacggcagca actacgcgca
     4741 agatctgacc atgttggaca tgtgcctgta cgatggtttt aacggtaatg ccatcagtta
     4801 tgaaatcatg ctcaaagatg aagggcgacc agctgcaggg cgcagagacg gttacttctc
     4861 tatctatcgt cagggaggga ccaccaccga cgagggagaa cgcattgatt accgggtcaa
     4921 aatgtacaac ccggaaaccg gtgggcaaat tgatgtgcgc aataatgaaa atatggtctg
     4981 gaacagcatt aacctgaaac gtgtgcgtcc ggtggtactg cccggtattc gctatgccgt
     5041 gatgtgtgtg ccgacaccct tgactctggc agtcgataaa ttcagcgtga tggataaaca
     5101 ggccgggtac tacatgggca aattgtcagt aatctttacg ccttccttgc caaccatcaa
     5161 ttaaccctgc aaaccggaca aacgcttttt tctatataaa cgacgaagcc gtctgataaa
     5221 gcggcttcgt cattacaact taagttaaac gttaatgttg cggtgcggga tcgttatgtg
     5281 tttcctgcgc cacctcaatc gccggtttgt tctgcaacac ggtccagata accagcgcac
     5341 ctaacaggtc gaacactgcc agaactgcga acagcgggct gaagccgatg gtgtcagcca
     5401 gtgcaccgac aaccagcgca aacagcgtac ttgccagcca tgcggacatc ccggttaaac
     5461 cgttcgccgt cgccacttcg ttacgaccga acacgtcaga agagagcgta atcagcgcgc
     5521 cagacagtgc ctggtgggca aaaccaccga tacacagcag cataattgcg acatacgggt
     5581 tggtgaacag accgatcata cccgggccaa tcatcagcac agcacccagc gttacgacca
     5641 tcttacggga aacgatcagg ttcacaccaa accaacgctg gaacagcggc ggcaggtaac
     5701 caccgaggat acaaccgagg tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct
     5761 ctttcaggtt aaagccgtaa actttaaaca tgaacagcgg gatccacgcg ttaaaagtac
     5821 cccaggccgg ttctgccagg aaacgcggca gcgcgatacc ccagaactga cggttacgca
     5881 ggatctgacc aacggacatt ttcttcgccg tgctcacctg gtgctgggct tcctgaccat
     5941 taataatata gtcgcgttct tcatcggtca gatgcttctg gtcgcgcgga tgtttataga
     6001 aaatcagcca tgccatcgcc cagataaagc tcaatgcacc ggagatgata aatgccatct
     6061 gccagctgtg cattacgatt gcccatacca ccagcggcgg cgcaatcatc gcaccaatcg
     6121 aagaacctac gttaaagtaa cctaccgcga tggaacgctc tttcgccggg aaccattcgg
     6181 agctggcttt cagacccgcc gggatcatcg ctgcttccgc ggcaccgact gcaccacgag
     6241 caatcgccag gccaccccag ctaccagcca gcgcggttgc accacagaac acggcccaca
     6301 gcacagcaaa cattgcataa ccgattttcg tacccagcac atccagtaca taacctgcta
     6361 ccggttgcat gaccgtataa gcagcagaat aggctgcgat gatataggaa tactgttggg
     6421 tggagatgtt taactcttcc atcagagttg gcgcagctgc cgccacagtg ttacgcgtca
     6481 ggtaaccaag cacggtgccg agcgtcacca gtgcgatcat ataccaacgt aaccctttaa
     6541 ttttacgcat gtaaaacctc atcgttttgt tatatccgca ggaagcgcgg ccatcatccc
     6601 gccagaggct gaagatgtta gttacgaccg ggtaaccgaa aaaaaaccgg cacgccccga
     6661 acgttgccat gaaagtttcg tgctaattcg gcttccgtac cggttaaccc gattcatcta
     6721 acaaatgaat gaatcggcaa tttgcattcc gtcgacttat gatttgcgac ggcagaaaga
     6781 taacttgtca tacaacttta aaaggtgaga gccatcacaa atgtgcgaat atttgtaggg
     6841 acattacctg acgacagcga ggccagtact ggcgcggcct gcagagagat ttaccacttt
     6901 gagagtaatt tttttaacta cgtttattga tctaactcac gaaaatatct tcggactctg
     6961 gaaattggtg tgataacttt gtcagcatcg caccataagc aagctagctc actcgttgag
     7021 aggaagacga aaatgactcc gtttatgact gaagatttcc tgttagatac cgaatttgcc
     7081 cgccgtctgt atcacgacta cgcaaaagac cagccgattt tcgattacca ttgccatttg
     7141 ccgccgcagc agattgcgga agactatcgt tttaaaaacc tgtatgacat ctggctgaaa
     7201 ggcgatcact acaaatggcg cgctatgcgt accaacggtg tggccgagcg tctgtgtacc
     7261 ggtgatgcgt ctgaccgtga aaaatttgac gcctgggcgg cgactgttcc gcacactatc
     7321 ggcaacccgt tataccactg gacgcacctc gaactgcgtc gtccgtttgg tatcactggc
     7381 aaattgcttt ctccgtcaac tgccgatgaa atctggaacg aatgtaacga actgctggcg
     7441 caggataact tctccgcgcg cggcatcatg cagcagatga acgtgaaaat ggtcggcacc
     7501 accgatgacc cgatcgattc tctggagcat cacgcagaga tcgccaaaga cggctctttc
     7561 accatcaaag tgctgccgag ctggcgtccg gacaaagcct tcaacatcga acaggcgacc
     7621 tttaacgact acatggcgaa gctgggcgaa gtttccgata ccgacattcg ccgctttgct
     7681 gacctgcaaa ctgccttgac caaacgtctg gatcacttcg ccgctcacgg ctgtaaagtg
     7741 tctgaccatg cgctggacgt ggtgatgttt gctgaggcga acgaagcgga actggacagc
     7801 attctggcgc gccgtctggc tggcgaaacc ctgagcgagc acgaagtggc acagttcaaa
     7861 actgcggtac tggtgttcct tggtgccgaa tatgcacgtc gcggctgggt acagcagtac
     7921 cacattggcg cgctgcgtaa taacaacctg cgtcagttca aactgctggg gccggatgta
     7981 ggctttgact ccatcaacga ccgtccgatg gcagaagagt tgtctaagct gctgagcaag
     8041 cagaacgaag aaaacctgct gccgaaaacc attctgtact gcctgaaccc gcgcgataac
     8101 gaagtgctgg gcaccatgat cggtaacttc cagggcgaag gtatgccggg caaaatgcag
     8161 ttcggttccg gctggtggtt taacgatcag aaagacggta tggaacgtca gatgacccaa
     8221 ctggcgcagc tcggtttgct gagccgcttt gtcggtatgc tgactgacag ccgtagcttc
     8281 ctgtcataca cccgtcacga atacttccgc cgcattctgt gccagatgat cggtcgctgg
     8341 gtggaagcgg gcgaagcacc ggcggacatc aacctgctgg gcgagatggt gaaaaatatt
     8401 tgctttaaca atgcgcgtga ctacttcgcc attgaactga actaaggtct ggggttgata
     8461 tgcaatacat caagatccat gcgctggata acgtcgcggt cgctttagcg gatttggctg
     8521 aaggcacaga agtcagtgtc gataaccaga ctgttacgct gcgccaggat gttgctcgtg
     8581 gacataaatt tgcgttaacg gatatcgcaa aaggggccaa tgtcatcaaa tatggcctgc
     8641 cgattggtta tgcattggcg gatattgcgg cgggggaaca cgtccacgcc cacaatacgc
     8701 gcacgaatct gagcgatctg gatcagtatc gctatcaacc tgattttcag gatctgcctg
     8761 cgcaagcggc agatcgtgaa gtgcagatct atcgtcgcgc taacggcgat gtcggggtgc
     8821 gtaacgagct gtggatcctg ccaaccgtgg gctgtgtcaa cggcatcgcg cggcaaatcc
     8881 agaaccgttt cctgaaagag accaacaacg ccgaaggtac cgacggcgtg ttcctcttca
     8941 gccacaccta cggctgctca caactgggcg acgatcacat caacacccgc accatgctgc
     9001 aaaacatggt gcgccacccg aacgcgggcg cagtgctggt gattggtctg ggctgtgaaa
     9061 acaaccaggt tgccgcattc cgtgaaacgt tgggcgatat cgatcctgaa cgcgttcatt
     9121 tcatgatctg ccaacagcag gatgatgaga tcgaagccgg gatcgagcat ttgcatcagc
     9181 tgtataacgt gatgcgcaac gataaacgcg agccaggcaa actcagcgaa ctgaagtttg
     9241 gtctggagtg cggtggttct gacggtcttt ctggtattac tgctaacccg atgctggggc
     9301 gtttctctga ctacgtgatt gctaacggcg gtactaccgt actgaccgaa gtgccggaga
     9361 tgtttggcgc agagcagttg ctgatggacc attgccgcga cgaagcaacg tttgaaaaac
     9421 tggtgaccat ggtcaacgac ttcaaacagt actttattgc ccatgaccag ccgatctatg
     9481 aaaacccatc gccggggaac aaagcgggcg gtatcaccac gctggaagat aaatcccttg
     9541 gctgtaccca gaaagcgggt tccagcgtcg tggttgacgt gctgcgttac ggcgagcgtc
     9601 tgaaaacgcc ggggctgaac ttgttaagtg cgccgggtaa cgatgccgta gcgaccagcg
     9661 ccctggcggg tgcgggctgc catatggtgc tgttcagtac tggtcgtggc acgccatacg
     9721 gcggctttgt accgacggtg aaaatcgcta ccaacagtga actggcggcg aagaaaaaac
     9781 actggatcga ctttgacgcg ggtcagctga tccacggtaa agcgatgccg cagttgctgg
     9841 aagaatttat cgacaccatc gttgagtttg ccaacggtaa acaaacctgc aacgagcgta
     9901 acgacttccg cgaactggcg atcttcaaaa gcggcgtaac gctataaagt cgaaatgagt
     9961 tgttaataaa aaacggcgtt tcataatgtg aagcgccgtt tgcttttcca tacctgataa
    10021 ggatttatca tgaccgcgta ttggctggcc cagggcgtgg gtgtcatcgc ctttctgatt
    10081 ggtatcacaa catttttcaa tcgtgacgaa cgtcgcttca aaaagcagct ttcggtctat
    10141 agcgccgtta ttggcgtaca tttttttctt ctgggcacct atcccgctgg tgccagtgcc
    10201 atccttaatg ccattcgtac attgattacc ttacgcacgc gcagcttatg ggtaatggcg
    10261 atttttattg tgctgactgg cggaattggc ctcgcgaagt tccatcatcc tgtcgaacta
    10321 ttgccggtta tcggtacgat tgtcagtacc tgggcgctgt tccgctgtaa agggctcacc
    10381 atgcgctgcg tgatgtggtt ttcaacgtgt tgctgggtga ttcacaactt ctgggcgggg
    10441 tcgataggcg gcacaatgat tgagggtagt tttctgctta tgaatggctt gaatatcatt
    10501 cgtttctggc ggatgcagaa aaggggaatt gatccgttta aagtagagaa aacccctccc
    10561 gccgtagacg aaaggggtta aacaattaat tacgcagggc gctatttgcc agacgatcgt
    10621 cttccgcctg gcaagctgcc gcagtgaaca gcacgtcagt tgaagagttc agcgcggttt
    10681 cgcaagagtc ctgcaatacg ccgatgataa agccgacggc aaccacctgc atggcgatat
    10741 cgttcgaaat accgaacata ttacaggcca gcgggatcag cagcagagaa ccccccgcca
    10801 cgccggatgc gccacaggca cacagagaag ccaccacgct caacagcagc gccgtgggca
    10861 gatcgaccgg gatacccagc gtattaaccg cagccagcgt caacacggta atggtgattg
    10921 ctgcgcccgc catattgatg gtggcaccca gcggaataga aacggaatag gtatcgcgat
    10981 ccagattcag cttttcacac agtgccatat tcaccggaat gttcgccgca gagctgcggg
    11041 tgaagaaggc atacacgccg ctttcgcgca ggcacagcag caccagcggg aacgggttac
    11101 gacgaatttt ccaccacacc agcaatgggt taaccaccag cgccaccagt aacatacagc
    11161 caaccagcac gaccagcagt tgcgcgtagc cccacagtgt ggagaaaccg gtggttgcca
    11221 gggtagaaga aaccagacca aaaataccaa tcggtgcgaa gcgaatgacc agtttcacca
    11281 taaaggtgac ggcattcgac atatcgttga ccaggttttt ggtggtctcg ttaccgtgac
    11341 gcagtgcgaa accgaggcca attgcccaca ccaggatccc gatgtagtta cctttcagca
    11401 gcgcgtcgat ggggttggaa accatgctca ttaccagccc gcgcatcact tctacaatgc
    11461 ctgacggtgg cgaaatatca cccgcgctac tggataagtg cagggtagaa gggaaggcga
    11521 agctgaaaac gactgcggcc agagcagcag agaaggtacc cagcagatag aggaacaaaa
    11581 tagggcggat attggttttc tgcccgtgct ggtggttagc aatagatgcc atcaccagca
    11641 tcaacaccag gatgggggca acggctttca gtgcgccgac gaacaaagta cctaacagac
    11701 caacagcttc cgccgcgggt tttgagatcc atgccagaag aatccccaga acaaggccga
    11761 ccaggatttg ttttaccagg ctgccatgag ccagacgccg gaatagcccc ggtgaacgtt
    11821 gcgtagtcat ttttcgatcc tttcattgtg ttgtcgcaca tccctggtgc gttctgtcga
    11881 cgcatctttc aggatgtaac aaagtgtttg caaggtcgag tataaggaaa gttacagctt
    11941 cgggaagcga aaaatgctgg attttaaaga gaggtcatat tttgcaactt aatggttaca
    12001 tttatttgac ataaaaataa agccaacgtg cggcttccag actgatgaca acatcaaaaa
    12061 aggactcggt cagtcccctc gccccgtttg gggagagggt tagggtgagg ggaacaggcc
    12121 agcatcggtg cgaaaaatta ccctcacccc gaccctctcc ctggaaggga gagggagaaa
    12181 acggtgctca tgttaatgac agggtacacg gcttaggcag attaaaaatt atccaccccg
    12241 ctgcttatca tgccgataat tcacccaggc gttgataata aacgtcatca ccagaatgcc
    12301 aaacaccacg cccagcgaga cggcgattgg aatatggtag aagtcgacaa tcagcatctt
    12361 gataccgata aacaccagaa tcaccgccag gccatatttg agcatcgaga aacgctctgc
    12421 tacgcccgcc agcaggaaat acatcgcacg caggccgagg attgcaaaca ggtttgaggt
    12481 cagcacaata aacggatcag tggtcacggc gaagatagcc ggaatgctat ccacggcaaa
    12541 aatcacgtcg ctcaattcca ccagaatcag taccagcatc agcggtgtgg cgtacagcaa
    12601 tccattctta cgcacaaaga aatgctcgtt gtcgatggtg tcggtcatgc gcaaatgacc
    12661 gcgtagccag cgcaccagcg gcttgtcgcc aatgcctgat tcatcttcat gggcgagcgc
    12721 catcttcacg ccggtaaaca gcaggaaggc accgaagata tacaggatcc agtcgaactg
    12781 tgaaatcagc cagctgcctg tgaagatcat gatggtacgc agaacaatcg ccccgagcac
    12841 gccatacacc agcacgcggc gttgtaatgc cgccggaaca gagaaatagc tgaacaacat
    12901 cagccagaca aagacgttat cgaccgccag cgatttctca atcagatagc ccgtgagaaa
    12961 ggccagtgcc tgtggatcgg caacggcgcg accttcagtt tgcaccagat accaccagaa
    13021 agcggcgtta aacagtaacg acagcgtcac ccagaccagc gaccaggccg cagcctgttt
    13081 catggtcatg gcatgtgccc cacgacgccc ctgcaacaac aggtcgatag ccagcataat
    13141 ggcgacaaca acagcgaatc cgccccatag caacggcgtg ccgacagtat tcatagaagt
    13201 tccttacaca taaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata
    13261 gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct
    13321 ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca
    13381 atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagcc agtcaatggt
    13441 ctgttacata ccttttacac tgttttactt tggcaattat agttttacgc tatctgccgg
    13501 aaaaatcacc ccagtctggc ggcggatctc ggtcagcagt ttggcggtga tgcgactgac
    13561 cgccagcccc ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc
    13621 gtagagcatg gtattaatat gctgcggctg ggtgagatcc tgcatttggc tgccacgcgg
    13681 cacgaagcac actttctggc attcagacag tttttcaatc accagcgatc ctgcttcgcc
    13741 ctgaatctcg ctcgccagga cagaatcact gacttttgag tgctgcaagg tgacgctgaa
    13801 atcaccgtaa tccatcacca ccacgccgtg ggcatctaca ccgcttgcca gcaaactggc
    13861 ggttgcctgc acgcttttcg gctcaccaaa taacgccacc gccgacgcca ggcagtaaaa
    13921 gccgatatcc ataattgaac cgttagagaa tgacggatta aaggtgttgg gattttcacc
    13981 gtccaggtaa cgttgatagc gcgaggaata ctggcaatag ttgaaaaaga ctttacgcaa
    14041 tttgccgact ttcggcagcg cctggcgcag caaatgaaag ttcggcaggc aggcggtttt
    14101 aaatgcctca aacagcacca cctgattttc ccgcgcacag gcaatggcgg catccacttc
    14161 cgccagattc gacgccagtg gtttctcgca aatcacatga attttatggc taaggaaaag
    14221 ttgtgtctgg gaaaagtgca gggcattcgg gctggcaata tacaccgcgt caatggcatc
    14281 gctttccgcc atcgcttcca gcgaggtaaa cagatgctcg acagaaaaat cattggcgaa
    14341 gtgctgagcc tgctcaaggc tgcgggaata tacggcggtt aacttgtatt taccgctctc
    14401 atgggcggcc tcgacgaact ggcgagtgat ccagttcgta ccaatcacag cgaaacgtat
    14461 cataacgcgt ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc
    14521 ttcgacgtgc gcagatacgc attattcttt tgccggacag ccatgctcac taaacggccc
    14581 aatcatgacc gatggcccga gatatttcgg ctggcgatgg aggattttgg cgtcaatcac
    14641 ggcgctgaca cgagcgagtt tctcccgcag ttgagtataa aaaccgtgct tcatattgag
    14701 gtcaggcgcg ttaaaaccga tagcgtcgat gccgtactgt tttgccagcc agatggcgcg
    14761 ttgattatga aattcttgcg agataatggt gatatggttt tcaccaaaaa cctttttggc
    14821 tcgcactacc gaatccagcg ttgagaatcc ggcatagtcg cagaagatca ctttcgccgg
    14881 tacgccttta gcgatcaagc ctgcatacct gaggcttcat cgtaattttt acgtccgtta
    14941 tcgccactta ccagtagcca tttcactttt ccggcgtggt acaacgctgc tgcggtatca
    15001 atacgtcggg taaagtagcg attacccggc ctggccccca gcaataagcc cacgttacgc
    15061 gccggaactg cgttgacatc gctccaggtc agctgcttgc tggcgttgac cataacgcga
    15121 tcggcaataa aaatggtcgc tcctgctata agcaacaaca aacagccaat cttaagagtc
    15181 cggcgagaaa aacaaatacg gagaagaagg cggggctgca tggtcgttat ccttaacgaa
    15241 aggggggagg tcgattttag gcgctaaaga aagaaaagat aacgcctgac atcattttca
    15301 gattttgtcc tgcccaagcg acaactgcgt tagccacagc cgggtgtcga actcaagctg
    15361 gtggtactgc ggctccatat ggcagcacaa ctggtaaaac gctttgttat gctctttctc
    15421 cttcaggtgc gccagttcgt gcaccacgat catacgtaaa aacggttccg gcgcgttgcg
    15481 aaacacagta gcgacgcgga tctctgcttt tgctttcagc ttgccgccct gcacacgaga
    15541 aacagcggta tgtaacccga gcgcgttttt tagcacatgg atcttattgt catacatcac
    15601 tttattgatc ggcggggcat tgcgcagaaa ctgatttttt agatccggag tatattgcca
    15661 gagggcttta tcggtggcgt agtcgtgtgt tcccggatag cgttttgcca gcacatcgcc
    15721 cagacgctgt tcgttaatca gcgttcgcac ctgggaaagt agctgctcgg gataaccctg
    15781 aagataagta agattgctca taaccgccca cgaaaaagag aaaaagggta tactcacgca
    15841 cccttttcag gggcaacgtc gaaattttat cattcaggag ggccgatgag ccacttagac
    15901 aacggtttcc gttcactgac actacaacgt tttccggcga cggatgacgt taacccgcta
    15961 caggcgtggg aagcggcgga tgaatatttg ctgcaacagt tggacgacac agaaatccgc
    16021 ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa gctgcgcgct ggcggaacat
    16081 aagccgtaca gcattagcga ctcatacatc agtgaactgg cgacgcgcga gaatttacgc
    16141 ctcaacggga ttgatgagtc gagcgtgaag tttctcgaca gcaccgccga ctacccgcaa
    16201 cagccgggtg tggtgctgat caaagtgccg aaaacactgg cattgctgga acaacaactg
    16261 cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta ttgccggtgc caaagcccgt
    16321 gacattcaca cttccacgct ggaactgttc gaaaaagtgc tcggcccgac caccaccacg
    16381 ctggcatgga aaaaagcgcg cctgattaat tgcacgttca atgaaccgcc gctggccgat
    16441 gcgccgcaga ccgttagctg gaagctggaa ggtactgact ggactatcca taaccatgcg
    16501 aatgtcttct cccgcaccgg gcttgatatc ggcgcgcgct tctttatgca acatctgcca
    16561 gagaatctcg aaggggagat tgtcgatctc ggttgcggta atggcgttat tggtctgacg
    16621 ctgcttgata aaaacccgca ggcgaaagtg gtgtttgtcg atgaatcgcc gatggcggtt
    16681 gcttccagcc gtttgaacgt tgaaaccaac atgccagagg cgttggatcg cagcgagttt
    16741 atgatcaaca acgcgctctc cggcgtggag cctttccgct ttaatgctgt gctctgcaac
    16801 ccgccgtttc accaacaaca tgcgctgacc gataacgtcg cctgggagat gttccaccat
    16861 gcccgccgct gcctgaaaat caacggcgag ctgtatattg ttgccaaccg tcacctggat
    16921 tacttccata aactgaagaa gattttcggc aactgcacca ccatcgccac gaataataaa
    16981 tttgtggtgc tgaaagcagt gaagctgggg cgtcgtcggt aagattggga gttacctgcc
    17041 ggatgcggcg ttaacgcctt atccggccta cgttcagcac gttactccca ggccggataa
    17101 gacgcggcaa gcgtcgcatc cggcaatgtc tgcccagttc tggaatcgcg cttccataaa
    17161 acaaaaaatc cctgtcacca tcctctacat tctcttgttt agcgttgttc aacgtttatg
    17221 ctacatacgt gaagttatga cgcataaaga atacgatttc tttaccgctg ttcatcgtac
    17281 taaggggaaa aaatgattgc gattgccgac atcttgcaag caggagaaaa gctaactgct
    17341 gtggcacctt ttctggcggg tattcagaac gaggaacaat acacccaggc gctggaactg
    17401 gtagatcatc tgctgctcaa cgatcctgaa aaccccttgc tggatctggt gtgtgcaaaa
    17461 ataaccgcgt gggaagaatc agcgcccgaa tttgcggaat ttaatgccat ggctcaagcc
    17521 atgcctggcg gtatagccgt gattcgtacc cttatggatc aatatggttt aaccctttcc
    17581 gatctgccgg aaattggcag taaatctatg gtgtcacgcg ttttgagcgg gaagaggaaa
    17641 ttaacgctgg aacacgctaa aaaattggca acgcgattcg gcatttctcc cgccttgttt
    17701 attgattaac gcgacgtgcc ggatgcgttg catccggcgc ttcaaataac gatttaaatc
    17761 tccagcgcca gccgtgttcc ctgggcaatt gcccgtcgtg cgtccagctc catagccaca
    17821 tcgcagccgc caattaaatg cacagttttc ccgctatcaa tcagcggttg cgccagcgcg
    17881 cggtttggct cttgccctgc gcagatcacc acattgtcca ctgctaatac ctgcgtttcg
    17941 ccgttgatca ccacatgcag cccgtcatcg tcaatcttct gataacttac gcctgggatc
    18001 attttcacac cccgcgagag cagggtggtg cgatggatcc agccggtggt tttgcctaac
    18061 ccctgtcccg gtttgctggc tttgcgctgg agcatcacaa tttgccgtgg gctacgcggg
    18121 atctgcattc cctgcgggct taagccacca gcctgttgta ggctgctgtc gatcccccac
    18181 tcattacaga acctggcgat attctggctg gtggattcgc ccggctgact taaatacatc
    18241 gccgtatcaa aaccaatccc gccacaaccg atgatggcaa ccttgttgcc taccggcgct
    18301 ttgtcgcgca gtacatcgag ataactcaat accttcggat gatcgatccc gtcgataggc
    18361 ggaacgcgcg gcacgatccc actggcgagg atcgtttcat cgaacgcctg taactgatcc
    18421 gccgtcacgg tgtgattgag tttcagcgtc acgcccgtca cttcgatcat ccggtggtaa
    18481 tagcgcagcg tttcgtaaaa ctcctctttg ccggggatct gtttggcgat attaaactgc
    18541 ccgccgatct cgctgtgggc atcaaacaat gttacatgat gcccacgcgc cgccgcgtta
    18601 atggcaaacg ccagcccagc aggtcccgca ccgaccaccg ccagattttt tttctgcacg
    18661 gcaggaagga ttggcatttt ggtttcgtgg caggcgcgag gattcaccag gcacgaggtg
    18721 actttgccaa cgaagatctg atcgaggcag gcctgattgc agccaatgca ggtgttgatc
    18781 tcatcggctc gccccgattg cgcttttgac agcagttccg catcagcaag aaacggtcgt
    18841 gccatcgata ccatatcggc atcgccgcgc gagagaatgt cgtcggcaac ctgcggatcg
    18901 ttaatccggt tggtggtcac cagcggcagc gaaacgtggc ctttcagttt gcgcgtgacc
    18961 caagtaaatg cgccgcgcgg cacgggcgtg gcaatggtcg gaatacgtgc ttcatgccag
    19021 ccaatgccgg tgttgataat ggtcgcgccc gccgcttcaa tggcctgcgc cagctctacc
    19081 gtttcggcaa aagtcccgcc gtcttcgacc aggtcgagca tcgacagtcg gtagataata
    19141 atgaagtcgt tgccgacgcg ttcgcgcacc gcacgcacta cttctacggc aaatcgcatc
    19201 cggttgcggt aatcgccgcc ccactggtca ctacgctgat tggtgcgcag cgtcagaaat
    19261 tcgttgatca aatacccttc ggaccccatt acctctacac cgtcgtatcc tgcctcccgt
    19321 gccagttgcg cgcagtgggc gaaatcgtcg atcagttgca ggatctcttc atgggtgagt
    19381 tcgtgaggaa cgaaacggtt gatgggggcc tgcaatgcgg aggaagcgac cagatgcggt
    19441 tggtagctgt agcgcccggt atgcaaaatt tgcagggcta ttttgccgcc ttcctgatgt
    19501 accgcttcgg taatggtgcg atggtgtggg atctggctgg cgtcgttgag cattgcgcca
    19561 ccttccatgc caacgcctgt taaatctggt gcaatgccgc cgctgacaat cagcgccacg
    19621 ccgtgacggg cgcgttcggc ataaaacgct gccagccgct cggcaccgtc cgggtattcc
    19681 tccagcccgg tgtgcattga gcccatcaac acgcggtttt ttaacgtggt aaaacctaaa
    19741 tccagcgggg cgaacagcaa cgggtagctc ataaaattgt ccagtatgta aaataattgt
    19801 tatgtggtcg gatgagttct aatttagccg tcgccggaga aaagggaaaa ggggagtgcg
    19861 gggtttgtga tgggattcaa aaagaatacg cccggtggta acgccggatg cgacgcttgc
    19921 cgcgttttat ccggcctggg ggattgcaca tgtagggcgg ataaggcgtt cacgccgcat
    19981 ccgccagtgg cacggtgcag atgccggatg cgacgcttgt cgcgttttat ccggccagga
    20041 ggattgcaca tgtagggcgg ataaggcgtt tacgccgcat ccgccagcag ttgcaaatta
    20101 ttgcttacgg aaaaaatcgt tatacaacat atacaaatgc gcggcactcc aggagaaatt
    20161 tggtgcgcct tgctgtgcgc cagtcagcgg gttgtaattc tcctgaattg ggccatcggc
    20221 ggttaatcct ttggcgtgac ggaagaacgt atccgccagt ttcagggcat catcacgata
    20281 accgtaacgc tccatccctt tcagaccaaa ccagaactga tccacccata cgcgcccgcg
    20341 ccagtagata tcagcgccaa aagccggatt ggttaacgcc gccgttccca gcgggacaaa
    20401 ggtgttgaac tctttaggat cgagcatcac cttcaccacc gcgtcggcgt tggcctgcgt
    20461 tgccgcaccg ttaaacagcg gcgaccagcc ttccggccct ttaccgcgct cgacaatcgg
    20521 tttgcccgcg cagccgttcg ccagcggttt atcttcaata cgcacgtcat agtagaactg
    20581 ggtagccggg tcgaacatac aggtgtttat gtagtctgcg agctgctgtg ccaactggcg
    20641 atagcgtttg gcctcttccg gcttaccgag gatggttgcc atctccgcca gataatggtt
    20701 atcgctgtac atatagctgg cctgatccac cgactcctgc aatagcgagt agcccagcaa
    20761 cgttccgtcc tgactgcggt tttcggcgaa tttcaccgtc cagtcgctac gtttgccgcc
    20821 attagcgaca tatttatcca actgttcttt gtcgataaaa ccaaagacgg cggcatcatc
    20881 acgccccgat tcccacgacg cagcgacctg tgccggaatt tccagactgt cgtactggcc
    20941 tttctccacc acgcgggcgt agttgttcag cccagactgc gtctcttctt tgttgccttt
    21001 cttcaccgta aacagcatct cgccgctctc agtgttgtgg gctttgtcgc gggtcgcgcc
    21061 atattacggc acgccgttgc cgttgtgatc gcggttacgt aaccaccagt cgtgataggc
    21121 caccagtttc gggtacatct ctgccaacca ggctttatcc tgggtgacgt tgtacacttc
    21181 catcaccgac caggcggcaa ggctgggttt ggtattacgt tcgttccagt tgccgccatc
    21241 gccaccacgc tcggggctaa ggttccacgc tatcaggtcg gggacaaagc ccacatcctg
    21301 cggacgcacg ctatcgccag gctggatctg ccaggagaag accgcgcgga tattctcttt
    21361 ggcgatgtcc ggattgaaat gcgccatcgc aaacgcctgc ttccaggtat cccacggcca
    21421 ggtctgattg ccggagaacc agcgcccggt caccgacggt gtaacggtgt taaatttcac
    21481 cgcaccgccc ggtgagcgcc agttaccgtt gagcgtttcg atcgctttca ccgcgacgcg
    21541 cgtctgttcc ggcgtcgcat ccggattggt taaccctttc ttcagatact cttcccagcg
    21601 ttgctgcgag gcggtgagat aaaacgccgg acgcgccaga atatcgcgga tctgcatttg
    21661 ctctttgcta acttcctgag aggtcagcag atgggaatag gtggtataga gcgtcgtcga
    21721 accgttgata tgtgccttac tggtaaagcg attgccgttg atttcagtct gcaccggcag
    21781 ggatttatgc acctgatatt ccgattcgcc ggaggtcagc agatcccagg tggcgcgcac
    21841 tttgccaaag gtgactttca ggccatcacg ggtggcgctg attttgcgct ggtagtcagg
    21901 gtattcgcca gcaatggttt tatcggaaag cggtttccct tctttcgctt ccagtttttc
    21961 cagcagttcg ccgtcccaca ccagatccag cggtttattg ctggtgattt tggtttccag
    22021 tagtgacgtg cgcggcgtgg cgaagcgcag agtcatttcg acttgcacat cttttgctgt
    22081 cagtttttgc accagcgcac cgggaatact gtatgcctcc agcgtaaagt cgactttctt
    22141 gccgtcctgc cagacggtca ggcggtcgaa attgctggcc ataaagttga tgtactcttc
    22201 cgtcagcagc gcaacgcccg gaaagccgcc catggtgtta gggccgtctg gcaacagatg
    22261 accatgccag gctccgagat cgaaaaacgg attaaaacgc tggtgatcgt cgtaatcgta
    22321 atccttcatg tactgcggcg cgccagtacg gttaatcacg tttttataat tgtcggcgtt
    22381 agcggcatgg gcggaaaaac ttatcagcag agcgcaggtt actggcgtta aaatagtttt
    22441 tattttcatg aaattacagt ccttattacc agtagaaata ttgcatcagg aagatttggt
    22501 cgctgggatc gtgagaggca tttttcatgt agaagcggct atcaaaagcc gtggcgaaac
    22561 gtccgccgcc gtattcgtac cagataccaa actgcatgaa agaggtggtt tcatcgtcag
    22621 cgtggtcagg gcgatgtttg gcgtaggtgt aagcggtgga gagataaacg ccctttaacg
    22681 cttcatacat ggcgctcacc atcatcccac tttcacttcc cggtacgtcc agcccgtcgg
    22741 cgcgtccggt atggtgccag gcgcgagcgg caaagttagg agttagcagg cccgtgagat
    22801 agagctgtcc tgtgccgtcg gtgatttcca gaccgttcat ccaggtgatg ccttcggcaa
    22861 ggtcatactg gatgtaaccg ttcatcattg ccggataggt gtatttgtcg tcatagcgat
    22921 cgtacttgcc aaaatgtagc caggctttgc tttcgtcatg atgtccggca ggtgtggcgg
    22981 tgacgctata acgaaagttg ccgcttaagt tctgcacctt cagcgcggtc ataatgtcgc
    23041 gggtattggg aatgacgtag cccagatcgg gggtgaagtc gccccaccat tgcaggtcat
    23101 ccagcgagga atcctgacgc agactgagca tgatttcggt gccatcattg gtacgataac
    23161 cgccatagaa acggttaatt ccgccctcga aaccacccca gctatggtct ggcacccagg
    23221 cattgccgtc gtggtcggcc tgcacggtcc agccttcgcc gtaaatcagg ccgaaccagg
    23281 agccgtactt catctccagg ccaccttcaa tataggtacc gtcgctgtat ttatgtttat
    23341 tctcaccctc taaatccata tatccaccaa cccctaattc gccgtaaaaa cgaaatgaag
    23401 gagaagatgt cgaggtaatt gcaggtgttt ttgtgtcgtc atgttctgcc ggacgtgctt
    23461 cttctcctgc cgcagaaaag gcgtagcaaa acggcagagc aagtaatgct ctgcatggtg
    23521 cagtgattaa tttcattgtg ttaatccatg agctaaagaa aaaatacagc cttttatcgt
    23581 tgtaattata aaaatataaa ggcagaaact attttccctc tcctcatcca ggaagaggga
//
LOCUS       DAZGOB010000070        22875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGOB010000070 DAZGOB010000000
VERSION     DAZGOB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.070"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(56..2179)
                     /locus_tag="ACHLIF_002937"
     CDS             complement(56..2179)
                     /locus_tag="ACHLIF_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HFO9606921.1"
                     /translation="MSDITANVVVSMPSQLFTMSRSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQFKGNLLSEDHSLGDSLVMHNPQFVDSTSTILSKKLTIDLRTLTDYGF
                     AEGNTGAQNKAAFQKAIDDATLPTEIVIPEGAFIVDPGITIKNTVTMIRGAGAYQSRI
                     FSTGTAAPIITQQDGVITFCEFRDFGLDGNGYAANGISLTEANHIKIENIDVVNTNNN
                     AILVNGYSIDIIGCRLFQNAGNGINVGGYCNNINIINSRIYGNGAGGVLLTPAYAEGG
                     MSVRVNGNNIEQNKFYGLLAYGVKGLNLDANYWERNGEIGYPYSVPESITVRADIHLI
                     ANNFTLIPDLSKINDTVSIRGNQQTAIGYASALPNQDGFIFTNYAKNLTIENNQLLDA
                     SKVNNLLAMYHNNLSSKVTDRLYLANNTVNSIGYVGSYDPATQNPDTAHLIDIANREL
                     TANYLDRNMLLWTAASGTTGTLIKTQNIYAGNYSFLVTTGDRVWGRTIDLNKSPELKG
                     KFVWFGAWVNDQGSASKLMFIINGAGQTDSTAPLAGNGKWSYVSCGVYIYETDTAINV
                     GIRNYGSGNVLINSPSLCAYGMPSNALQVEKTTFYLSSVPTSGFWDIGERVINSAPAS
                     GQPKAWTCNIPGGPGTYSFLSEGNF"
     gene            2301..2567
                     /locus_tag="ACHLIF_002938"
     CDS             2301..2567
                     /locus_tag="ACHLIF_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO9606922.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKMVFETLKTMYSNNKKET"
     gene            2638..3123
                     /locus_tag="ACHLIF_002939"
     CDS             2638..3123
                     /locus_tag="ACHLIF_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606923.1"
                     /translation="MKKSLLIIPLLLVGCAKVSDYQASCEQRYQKLSDMANCLDASVK
                     NDSRMASAPTPKLYVLAAKMLGKGVDEGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            complement(3138..4982)
                     /locus_tag="ACHLIF_002940"
     CDS             complement(3138..4982)
                     /locus_tag="ACHLIF_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO9606924.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTRDEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDSLHEVLQKTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNNAANAIRPILSSQITAEQQ"
     gene            complement(4982..6388)
                     /locus_tag="ACHLIF_002941"
     CDS             complement(4982..6388)
                     /locus_tag="ACHLIF_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO9606925.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIDRS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAKELTRVGVDPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(6398..7090)
                     /locus_tag="ACHLIF_002942"
     CDS             complement(6398..7090)
                     /locus_tag="ACHLIF_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO9606926.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(7093..7548)
                     /locus_tag="ACHLIF_002943"
     CDS             complement(7093..7548)
                     /locus_tag="ACHLIF_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO9606927.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VYGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(7548..8396)
                     /locus_tag="ACHLIF_002944"
     CDS             complement(7548..8396)
                     /locus_tag="ACHLIF_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFO9606928.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            complement(8396..9814)
                     /locus_tag="ACHLIF_002945"
     CDS             complement(8396..9814)
                     /locus_tag="ACHLIF_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFO9606929.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPADSGFMQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDPEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            complement(9823..10305)
                     /locus_tag="ACHLIF_002946"
     CDS             complement(9823..10305)
                     /locus_tag="ACHLIF_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFO9606930.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDEQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSDRYYPGDLPLIDGDIPNA
                     "
     gene            complement(10280..10465)
                     /locus_tag="ACHLIF_002947"
     CDS             complement(10280..10465)
                     /locus_tag="ACHLIF_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606931.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFERLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            complement(10508..11779)
                     /locus_tag="ACHLIF_002948"
     CDS             complement(10508..11779)
                     /locus_tag="ACHLIF_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFO9606932.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISGQNKNNLISGKATGRVGNYITVAVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATASVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADANSDSSGDVTVTLSGVPIYDTTNPQYNSVSRQVE
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            complement(11791..12675)
                     /locus_tag="ACHLIF_002949"
     CDS             complement(11791..12675)
                     /locus_tag="ACHLIF_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFO9606933.1"
                     /translation="MENELIIDGQVIDLSETQENAEETIIQTESQPENESQDDNGKEM
                     ATDPEKTEETPEDYALRIGDEEIQLNADDNDHIDGQPAPQWVKDLRKGFKETQKENRE
                     LRRQLEEALAKPAEHQQPQPDAIPPKPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQ
                     QKLRQQQEYQQRFQQRVEAHKQRAAKLPVKDYQEMEAIVLSELPPIQQEIIIHCADEG
                     SELLAYGLGKSQQLRQRVAAETDPIRAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGG
                     ADAKQDEFNKLCPGAKIE"
     gene            complement(12689..14815)
                     /locus_tag="ACHLIF_002950"
     CDS             complement(12689..14815)
                     /locus_tag="ACHLIF_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO9606934.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQQEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDLMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYEAEYGKKPPASLDVTSMTSWEYD
                     WFDEDVIYIAKYYEVRKESVGVISYRHPITGEIATYDSDQVEDIEDELAIAGFQEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            complement(14818..16230)
                     /locus_tag="ACHLIF_002951"
     CDS             complement(14818..16230)
                     /locus_tag="ACHLIF_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFO9606935.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHTKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDGP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            complement(16227..16652)
                     /locus_tag="ACHLIF_002952"
     CDS             complement(16227..16652)
                     /locus_tag="ACHLIF_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HFO9606936.1"
                     /translation="MATEKKKGGRPSDYMPEVANDICALLSSGESLRKVCERPGMPSK
                     TSVFRWLAEHQEFRDQYAKATETRADSIFEEIFEIADDVIPDAAEVAKARLRVDTRKW
                     ALARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            complement(16732..16974)
                     /locus_tag="ACHLIF_002953"
     CDS             complement(16732..16974)
                     /locus_tag="ACHLIF_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFO9606937.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            complement(17078..17449)
                     /locus_tag="ACHLIF_002954"
     CDS             complement(17078..17449)
                     /locus_tag="ACHLIF_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFO9606938.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQSHESLSLLTFCVLVLRNGFTVTGESACASQENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            complement(17733..18251)
                     /locus_tag="ACHLIF_002955"
     CDS             complement(17733..18251)
                     /locus_tag="ACHLIF_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9606939.1"
                     /translation="MNHQLANLDFRDMVVVSGDRVITTSRKVAAYFDKQHHHIIQKIE
                     KLDCSDEFLTSNFSRVTYEHKGNQYVEYEISKDGAMYIIMSFTGKKAAAIKEAFIKAF
                     NWMRDRLMEMAHSYQREHNELMLEFMKEKDVASMSGRLLNRWGRIKKPQLIARIKRLE
                     QQAQISIPGLPK"
     gene            complement(18457..18894)
                     /locus_tag="ACHLIF_002956"
     CDS             complement(18457..18894)
                     /locus_tag="ACHLIF_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9606940.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            complement(18891..19367)
                     /locus_tag="ACHLIF_002957"
     CDS             complement(18891..19367)
                     /locus_tag="ACHLIF_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO9606941.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(19351..19674)
                     /locus_tag="ACHLIF_002958"
     CDS             complement(19351..19674)
                     /locus_tag="ACHLIF_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO9606942.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            complement(20747..21370)
                     /locus_tag="ACHLIF_002959"
     CDS             complement(20747..21370)
                     /locus_tag="ACHLIF_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9606943.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(21367..22032)
                     /locus_tag="ACHLIF_002960"
     CDS             complement(21367..22032)
                     /locus_tag="ACHLIF_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO9606944.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKAGELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQLIW
                     NRERISNSQDGIVKEIKGADMFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIHVQG
                     EGA"
     gene            complement(22010..22216)
                     /locus_tag="ACHLIF_002961"
     CDS             complement(22010..22216)
                     /locus_tag="ACHLIF_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO9606945.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            complement(22213..22818)
                     /locus_tag="ACHLIF_002962"
     CDS             complement(22213..22818)
                     /locus_tag="ACHLIF_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO9606946.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHSRHRWTIEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            complement(22811..22858)
                     /locus_tag="ACHLIF_002963"
     CDS             complement(22811..22858)
                     /locus_tag="ACHLIF_002963"
                     /inference="COORDINATES: protein motif:HMM:NF017611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO9606947.1"
                     /translation="MSDPNSSMYEEEYDG"
BASE COUNT         5421 a         5700 c         5208 g         6546 t
ORIGIN      
        1 actgattttt tttaaacata ccagatcaaa gtaatcattt caatacatag gtcttttaaa
       61 agtttccctc tgataagaaa gaataggttc ctggcccacc tggaatattg catgtccatg
      121 cctttggttg tcctgaagca ggtgcgctat tgataacacg ctcacctata tcccagaaac
      181 cggatgtagg tactgacgaa agataaaatg ttgttttttc aacttgcaac gcgtttgatg
      241 gcattccata tgcacataga gaaggtgagt ttatgagtac atttcctgaa ccataatttc
      301 ttataccaac atttatagct gtatcagttt cgtatatata cacaccacaa ctaacatagc
      361 tccatttccc attaccagct aaaggtgctg tagagtctgt ttgtcctgca ccattaatta
      421 taaacataag ttttgacgct gatccctggt cattgaccca cgcaccaaac catacaaatt
      481 tcccttttag ttctggtgat ttgttgaggt ctatcgttcg tccccaaact ctatctcctg
      541 tcgtaactaa gaatgaataa ttaccagcat agatgttttg agtttttatc aatgttcctg
      601 tagttccaga tgccgcagtc cagagcaaca tatttcgatc aagatagtta gcagttaact
      661 ctctgttcgc aatatctatt aaatgcgcag tgtcagggtt ttgtgtcgcc gggtcataac
      721 taccaacgta tccgatggag ttcactgtgt tgttggcaag atatagcctg tcagtaactt
      781 tggacgaaag attattgtgg tacattgcta ataaattatt cactttcgat gcgtctaata
      841 actgattatt ttcaatcgtc aggtttttcg catagttcgt gaaaataaat ccgtcttggt
      901 taggtagcgc gctggcgtag ccaatcgcgg tctgctgatt cccgcgaata gatacggtat
      961 cattaatttt tgaaaggtca ggaattaatg taaaattgtt cgcgataagg tggatatccg
     1021 cacgtactgt aatagactca ggtacactat acgggtaacc aatctcaccg ttgcgttccc
     1081 aatagttagc atcaaggtta aggcctttaa ccccgtaagc caaaagtccg tagaacttat
     1141 tttgctctat attgttgcca ttaactctga cgctcatacc gccctcagcg taagctggcg
     1201 taagtaaaac tccaccggct ccgttaccat agatacggct gttgattata ttaatattat
     1261 tgcagtaacc accaacatta ataccgttac cggcgttttg aaacaaccgg cacccaatga
     1321 tgtcaatgga gtaaccgtta actaatattg cgttgttatt agtattaacc acgtcaatat
     1381 tttctatttt aatgtggttg gcttcagtaa gactgatgcc gttagctgcg tagccatttc
     1441 cgtcaagacc aaagtccctg aactcgcaga acgtaattac cccatcttgt tgcgtgatta
     1501 tcggtgccgc tgtacccgta gagaaaattc tggactgata tgcgccagca cctcgtatca
     1561 tggtgacagt attcttaatc gtaataccag ggtcaacaat gaacgccccc tctggtataa
     1621 caatctcagt tggcaacgta gcatcatcaa tagccttctg aaatgcagct ttgttctgag
     1681 caccggtgtt tccctcagca aacccgtagt ctgtaagcgt cctcaggtcg atagttagct
     1741 tttttgagag aatggtcgat gtgctatcga caaactgtgg gttatgcatg actagagagt
     1801 cgccaagtga gtgatcttcg ctcaggagat tgcccttaaa ctgatctgga tcatacttta
     1861 gcacattcgg aaaatagaac tgctgcgcac cgtatgcatc ataaacagcc atagaatggc
     1921 cttgcacagt tacgaacttg gcaatctgtc cgttatatac agggtaacca gcagcgttaa
     1981 tgatgattgg ttgcgaaaca ggaacgtgag aaccgtcttc gttttccaca taaacctgaa
     2041 tctggttttc tggatttacc gggtcagtgt caattttacc gatataaatt tttccattgg
     2101 ctacggcttt aaaagagcga gacatagtga agagttgcga aggcatgctg accacaacat
     2161 ttgcggtgat atctgacatt tcattgctcc agaagaatga tatgatgcaa ccatgatgtg
     2221 attgcatacc gaaatggtac tattgagtat ttatccagta ggttacgatg ccattccacc
     2281 caactggtga ggcatcaagg atgtacagca aatacgacga ggcgcagttt cacttgagac
     2341 ttccgcatga actccacgcg aaaattaaac agcgcgcgaa gatgaataac aggtcgctga
     2401 actcagagat aattgcagcg attgaagaat cgttggctaa acaaagctct gcatccgttt
     2461 acattgatga tgcagagcgt atggcagaac aacaatctga tatggttaag aaaatggttt
     2521 ttgaaacgct taagaccatg tatagcaata ataaaaagga aacatagaaa tctagtttcc
     2581 ggctaaaatg gcattgcctt catgatatcc tgtgaaaaac taaggagagt taaccatatg
     2641 aaaaaatcac tgttaattat cccgcttctg ctggttggat gcgcaaaagt aagtgactat
     2701 caagcaagtt gcgaacaacg ctatcaaaag cttagcgata tggctaattg ccttgatgcc
     2761 agtgtgaaga acgactcacg catggcatca gcaccaacac ctaagctgta tgtccttgct
     2821 gcaaagatgc tcgggaaagg tgtcgatgaa ggcaagataa gtgacgcaca ggcaagactt
     2881 gagcttcaga atctttatgt tcaattacaa agccaagaac aagcccaaca aatagcacaa
     2941 agccaagcat tccagcaggc tttattgaat tatcaggctg taaacacaat gcaagcgatc
     3001 gagcaaaaag cgcgccagcc tgttataact caaccttacc caacacgcgt tgacacatat
     3061 acaaactgca attcaggatt tggaaacacg gtaacatgca acagtagcag taatatcagg
     3121 tagttacatc ttatctttta ttgctgttct gctgtaattt ggcttgagag tatagggcgt
     3181 atcgcatttg cagcattatt tagcgctctt tcataggctg gtgttcctgc tttggtgttt
     3241 gccaaacgta agagagcatt ccttgctgct ttggactcat acaagcgcat cattgcaccg
     3301 aaaccagcct caagccccat tgatacgcca agagtcgcag ttgcgccaat cgtccttatc
     3361 ctgttggctt gcgattgccc agtctgagtt actacatttg cggtgtctga ccttgctgtt
     3421 ttctgtagaa cttcatgaag agaatcaagc tctttcatgt gctttccaga aaaaatagtg
     3481 ttgtaaattt caccacctga ctgagatttc agcttattaa cttcagtgat gaacttggct
     3541 ggagagtcac cggccttttc cgctattttg ctgacgtaag ctgcacgcat agcatctttc
     3601 cctttatcat ccaatgcgct ccagattcgt ttcacgtcag atggttttct gcttaataca
     3661 acagtattta taagttcagg actggcttca ctgcttgcct tgttgagctt gttagcaatg
     3721 tttttattaa gcaccttatt ataaacgttt gcataatcgg aatttgcttt aaggtatttt
     3781 gctgcgtctg acgcaccgag gttttttgca actgcgttac gaaggtcttt tgacattgca
     3841 ttctctacca tattggtagc tgcttttgcc tggttgggga agaccatagc atctccctga
     3901 acattagatc taaatgctgt tctgtgctga cgcaagagat caaacgtaac atccaaatca
     3961 gttgcagggt ttgctaattc ttcacgcagg ttacgcaagg atgtaagcag gctttgattg
     4021 gctgaagtcc caagccgttc ctgtcttgcg atcgctgtat tcagagcatt catggtattt
     4081 gtagtatcaa ctgcggcatt acccatttta ttggtgacgt cattgataac agcgccagca
     4141 gcatccttcc gcccccttaa cgtggtggtc agagatttca ccacatcatc agggttgtac
     4201 tcaccaaaac ggtcaaaata attgcttacc agcttactac gcgttgcata ttgctctgct
     4261 cgctttgagc ccgtcccgag caaagccccc tcggcatcct gagtaaggcc gcgagtgaaa
     4321 gcatttttcg gcgggataac atcagatgtc attggtgtca cgcccatcga ttctgatgtg
     4381 gcaattttct tcgccacttc tggcgcaata tcacctttta tagccgttat tccacgccct
     4441 attccctttg ctgctgcgga aagaacaccc tgagcggcaa ggttaactcc ggcattttta
     4501 gctgcatttt gtgcgaaatc gcctttctga tttgcggcct ctgccagcga tccaatagcc
     4561 atgcttcctg ccgttccaac tcctggaact aaatacccac caattgtttc accggcttgt
     4621 gcgtatgggt ctgtcggttt gtctactgga cgataaacat catccaaaac ttttggccca
     4681 ccaagcccct gactgattgc attaatcaga cttgcgccac cctgcaatac gtcaaatggt
     4741 atgtttacca gaccacgacc agcctgttct gcaatttgcc ctgcactttg accaccagtg
     4801 agccaatcgc cagcttgttg catcaatgat ggttcgtccc gtgttggtgc attattggcc
     4861 tgattaactg tttgttgctg aacagcctga ccagcaaaat actcatcaat ggcggtgcca
     4921 atatcttcgg tgctcgtacc atcaggaaag gtaaatgtct taccgtttgc agttactttc
     4981 atcattccac cgtaaattga atgcctgatt ttgaggtata tgatccaacc tgattccgtg
     5041 gttctcctga aggtgtcgaa tcttgtgctg gcgctgcgtc agtattcatt gacatatacc
     5101 gcttaacggc actccccaat gattcacctt ttttaacatc caaccccaat atctgaccgc
     5161 cattacgcga ttgtccaggg ttgccattcg cgctcatcca ctcggcttta aactcattaa
     5221 actgcgcgtt tcgtcgctca aggtttgcca ttgcatcaag ccatcttgcg accgtctcag
     5281 ggttatccat gtcagttggc gcaccctgtc gaacgatctc aacgtcttta tccgttgctg
     5341 ggccgggagg taggaattta agaacctgac tgttaacaag ggcattttgg cgaatgcgca
     5401 aatcacgcaa tgttgtatcg cttccggtaa gttttgcgaa catgttctgt gcgttaccga
     5461 acaaacctgt cgttggtttt tctgctctga actgttgagc aagcgcactc atagaattgg
     5521 ctgagtttga tgatgctgta gcattgttta cagccgtctc gatgcctttt tccatgttta
     5581 ctgacagctt aggtgcttcg ctaatcaact gctgagcctt ttcctgcgct tgctgcatct
     5641 taaacccgaa ctcttgctga tccagagcca agcgttgtgc tgcgatattg tgcccagtca
     5701 ttgctgactg ataggaaagg ttttgccctc tcgcctgaag tgcttctcca gcctgattgc
     5761 tgcggattgt ctctgccagt ctgcctcggt caatttcacg accagccatc ttgtcctgaa
     5821 cattgaagta atcaattgga ccaagagcag ccattccaag gtgatcaaca aactcaccaa
     5881 atcctgaagg gttctgctga tacatctgag caacgctgtt agggtcaaca ccgacgcgag
     5941 tcagttcctt ggcgttgttt tgcagccatg attgcattgc ttctggagac gatgacgcaa
     6001 ggcgtgcgcc agccgctaag gtgccgatag aattacgctg gtcttcatca atgaatccca
     6061 tgcctttacg aacggattca atctggtctg gatattgagt agccaactga cgcaaagcac
     6121 cgcgatcacc agacgcataa gcattagcgt acgcctgctg aaattctttc tgccgctgag
     6181 cctgcttttc ctgctgaaac acccctgcaa tacctgaaag gccttgcaaa gcagtcagcc
     6241 caacattgtt agcgcctgaa cgatcaatat cattgttctg cctgataagc tgaagcgtat
     6301 tgccgatgtc atttacgctc ggagcgtttg agttgacgcc gccgatacca gccaacaatc
     6361 cgccgtttgt tccttgccaa gtagccatga ttacccctta aaacaacgag ccaagcaatc
     6421 cgataccagc accaatgcca gcgccccaag gcgttgatgt tcccaaaagg ctggcaagac
     6481 ctgcaccggc aatcgcacca gatgtgccgc cgctaattgc agtctgaaga cttgatggtt
     6541 tattggcatt agcagcggca agtgctgcgc tttgctgtgc aatgctgctc atgttgttgg
     6601 cgtacgtctg cccggcgttt gcctgacctt gcagtgcacc aagcccaacg tttgccagat
     6661 tgttgtaatt gctcatctga tttgataacc aagactgacc gagtgtcggc gcgatcgtag
     6721 ccagttgatt gcttgtggct gtcgaaccaa gtccacccgt cgcctccgca gcagcaagac
     6781 tctggtaacg agcctgacct gcaaggtctt tatactgctg agagttgtaa tactgattaa
     6841 gtgcctgccc ctgaccttct aaactggaaa gattctgaag ctggttaaca tactgctccg
     6901 caagcggcgt gaacggagca aggtttttca tgatcgtctg ccactgctga ttttgcaggt
     6961 ctgcggcata cttctgagct tctgctgcat actttgcgct tttatcagag ctgccacctt
     7021 tcccgccttt ttcagggcaa taaggttcct cgccgcgcag ttttctgccc agcttaaatg
     7081 catataacat agctatctcc cgtgattcag gaagtcgatt agttcttcgc gtgtggcgct
     7141 gtaaaacgtc acgtcatcca cgcctttgaa gtatttcttg atggttccta cacgcttaag
     7201 gccaatcatt gcgcagtaca tctgaccgtg gcggaatttg cgcgcagcga acgatgtgac
     7261 gcactgaacg gtggtgttag tcagaatgta tcgccagaac gccagcccga tttccttgct
     7321 gaatccacga acctctggca ggtacatggc gtggcaatcg aatgtaagcg gctgaatttc
     7381 ctgatagtaa acaatgccgc caaactgacc gtacacgtta acctcaaagt aacggcattc
     7441 aggcttgtag tcgtatccat caccgttgtt gcttccggca ataatgtcag ggtgatttcc
     7501 gactgcttct atcaggtcga tgtttcgcgt tggtttgaac tgaatcatta ctgctccgcg
     7561 attatcttga tggttgtggc agtaaacgcc gcaccatttg actgaatggt taacgtgctg
     7621 ccatttgtgg caagaaagcc gtctttatcc acgctgaaga acgtagctaa caggatgtta
     7681 tcggtcgttg tcgccgagtt gcgactgctt accagtgtgt caggaacaga gccggaaaat
     7741 gttagttgca ttgacctgtt ggcggttccg ctgggccacg tcccgacgat cgacagcttg
     7801 aagaacaagg ttttgttctc gttgaacaca accatcttgt tgttaacggt gtcgaagaat
     7861 ggtgccaacg agcctgatga cggcgtgagc gttttcagca ggctaacaag gttggtcggc
     7921 gctgtcggga tggttacaga tacgccagag taaacaacct ctgacttctt gcgagtagtg
     7981 gcatactcca gagcatcaat gcgcgattca tggtctgaaa cctgcgattc cagcgactga
     8041 actctggtat caagcgacgc aatatcgctt tcattctgag cgattcgcgt ttcatgttcc
     8101 tgaagagttg attctgcctg gctgattcgc tcctcatgat taacaagcgt tgcttccgca
     8161 gcagaaattc gctgctcatg gtcagcgaga atcacatcct gctcatcgtt cctgacttgt
     8221 gcgtcataag cgccctgtcc ggcctcgttg gccttgttag ccacattacc aacatcagta
     8281 ccctgtgcga taacgtaaag cagatacgac tgcgagaaga tattgcgtgg aaggactgat
     8341 gtgtcgagcc gtgtagcctg aatgattacc ggcacattga gattcgaatc cgccattact
     8401 caatccttat ctgagcgcca gacagagtga caggcgactt agtgataacg cgcagtttga
     8461 agccaacatt tttcctgatg cgccctactc gcttccacaa aacgcgtttg tcgtaaacga
     8521 acggttcatt ctgctcaatc atctgctcac gcccgtaatt tatgccgtca gtggttgcag
     8581 agaggaacag gcggtcagcg tactgagcaa cacccgtcga tgattccacc tccagatcaa
     8641 agcatctggc gttatccgct ttgaacaacg gagtaaacag caggtgttct tgctgtagcc
     8701 catactggct gctgatatcg aactgcaatt tcccggtaac cgattccagc ttatcgccgc
     8761 acgttatctg atttccttcg taaatgaagt cgatagcgcg gtacacatcg tcatacaggc
     8821 ctgttttcaa cacacaccat tgcggaccat tggcgcttga agatgcgtcg tacacgagga
     8881 cgtggcgcgg aaggtggata atcagcagtt catgcgcatc aaatcgcagc gattccatca
     8941 caccatcagc cagttcatca gcagtgtagg agcgaagaat tttctcaatg ctcgcgctgg
     9001 cgattggtga tacctgaccg gagccgatga tatacacaga cggcgcacct gttgccggat
     9061 tgctgatgaa cgcatacgaa tcggcgaacg gcgttttgca gtaagtcccg gcaatgcctt
     9121 tctgcaccat cagcgatggc tgtgcgacat acaaagcggc accaacggtg gttgcccccg
     9181 tcagggagaa atattcaatc gtcgatgaac caaagcagac gatgaagtct cgccatgttc
     9241 cgatgccgat gatgccgtca ggctgagact cggctcgata ttgtgcgctg tagcggtcag
     9301 gatgcgattc gtcttcaggg tcagtgataa accatgaatc agtgccgtct tttgaccacg
     9361 cataacgccc acgtaaacgc gtaatgtcgc gaacagaacc taactcatac tgcataaacc
     9421 cgctgtctgc aggccagttt gagacggttt taaccgtgcc atcatagcgg tattcgacca
     9481 gttgcccgtt aacgcctacc gcctgtgatg ttcgaccatg cgccattgat acgcgaccac
     9541 ttccggcaac atcaccgacc tcactttcgc ccttatacag tttgccacca cacacgcgat
     9601 aaacagcatt ctgtgccatg ttgtactcga cgcctcgcga tacgccgttc acatcagaac
     9661 gtttggcaat gcccgggaat gagcgaagat atccgctgct gttcaggatt tctttgggtg
     9721 tagccaacat attcactggc agatagtcga tatagtcggc gtttctaaag tctttgccga
     9781 cacctttcat aagcggaagt tgctgaatag gcatttattc acctatgcgt ttgggatatc
     9841 gccatcaatc agagggagat cgcctggata atatcggtca gatgtgaaca cgtcatattt
     9901 attaccctgc cctacaggaa aatctccacg tcgtcgcatt gaaggaacaa ccagagtgtc
     9961 ggtcatcaag gcatcatatg agcgttgggc gttactgaga acttgcggag ttggttcaag
    10021 gctgtaatca gatagcattc tcagcaataa ctgatagcct actgcgtgtt tgtattttct
    10081 tggaagacct gactcatcat ctggtaatgg ctgctcatct ccagttgcga aagcgtaacc
    10141 aatgtcgccg gggttaatca tccactcgga catcatatct tccagatcat ttacaccatc
    10201 ttcaattgat tgcggctcaa catcagtaag cgatgcatta gaagcaatag caaacttacg
    10261 aagcgcaaaa aggacgatct caccctttgt cagtactgtt gccattgtcc gccgccttac
    10321 gacctcgctt actggtcggt ttcaattcat caactgaggc aacaaagccc aacctttcga
    10381 aaaactggaa gtctttttct gcgataacgg cctgtacatg cccggattcg ttatctgcgg
    10441 caaggaatac actcatccga tccatattgt ttccttaaaa cataaaaggg gcgtaagccc
    10501 cttgttatta cggattaccg aagaactgac cgcccatgtg tgggttaaag cacacatatg
    10561 caggcagtaa gtcgaagcgc attttttgca cgttggcatc gccatctgcg tatttatgta
    10621 cgcggatgga gaaaccttca tatgttgcaa cagcagaatc aatactgtgc agtttcggca
    10681 gtgggataga tccgagtcca cagaagaact tgttatagaa caggtttggc ttcattgtct
    10741 ggctagcagt gcctactaca gatacggcat cgcctgcctc tacctgacga cttacagagt
    10801 tgtactgcgg gtttgtagtg tcataaatcg gaacaccaga aagcgtaacc gtcacatcgc
    10861 cactgctgtc tgaattagca tcagcagtaa ccgttgcagt gaagctaatt ggtgtggctc
    10921 cgttatacaa cgcctgtttg gtctgctgtt gcagccagta ggtattggtg aatttgacct
    10981 gatcaccagc tttcagaaaa cctgtaacgc tggctgtcgc tccggtcaat gttacagtga
    11041 actggtatga gtctttaact gcgttatagg taacagttgg ctgtgttttg actgtcagtg
    11101 ttccgccaaa tgccccctgc gtacgagagg caagcccatt agacatcagt gcgcgaatgc
    11161 cgccaaaatt ggttgggatc tgtgcgttct cccatgcagt acgaaccaat tgatctgaag
    11221 catgcaaacc agtctgcgca tcagcaagtc gctgtgcaga ccatggatcc attacagcat
    11281 agttttcacc ttcattaacg ccgaggtctt tcaggaaaga tgccgtctgc gcaacatcag
    11341 accatttggt gattggagta ttggggctac caagtgacaa cgcaccgtta ttcatcatga
    11401 agtgagcaag ctctgtttca aggtcggtaa cgattcgctg gcgaaccggc gcgagaattt
    11461 cttccagctg gttaagcttg atcgcttcct ccagttgctg atattcaaca gcaacagtga
    11521 tgtagttacc tacacgcccc gtagctttac ctgagatcag gttgttttta ttttgccctg
    11581 aaatatcacc agtgggagta cggagggatg agaattgatg cggacgttta aagctaacgc
    11641 tatcgccagt gctggagttg atttcacctg ccagcaactg acggtctacg gttttcgcca
    11701 gaactaaatc tgacataaaa cccggaagga attttttcag aacgatttga ctgacgttac
    11761 tgtcgagatt gttaggcatt tatcttttcc ttattcgatt tttgcgccgg ggcataattt
    11821 gttgaattcg tcttgtttcg catcagcacc gccaccacgt acttccggct ctggcttgat
    11881 ggctttcttt ggttttggag caaggcttac ctgtttgcta atctgcccca agaggaatgc
    11941 tgcgcgaatt ggatctgtct cagcggctac acgctggcgt aattgctggc tcttacctaa
    12001 gccataggcg agtagttcag agccttcgtc tgcacagtga atgatgattt cctgctgaat
    12061 tggtggtagc tcactaagaa caatggcttc catttcctga taatctttca caggaagttt
    12121 ggctgcccgt tgtttatgcg cttctaccct ttgctggaaa cgctgctggt attcctgttg
    12181 ctgacgtagt ttttgttgct gctgctgttc gacacggcct tttttctcat gccaatcagt
    12241 caatgcctgt tcaaacgcct gttcgtcata atcacacgac tcaagagtcg gttttggtgg
    12301 aatagcgtct ggttgtggtt gctgatgttc cgcaggcttg gctaatgctt cctcaagctg
    12361 gcgtcgcaac tcacggtttt ctttctgtgt ttctttgaag cctttgcgaa gatctttcac
    12421 ccattgcggt gcaggttgcc cgtcaatgtg atcattatcg tcagcgttaa gctgaatttc
    12481 ttcatcacca atacgcaagg cgtaatcttc tggtgtctct tcggtttttt caggatcagt
    12541 tgccatctct tttccgttgt catcctggct ttcattctca ggctgtgact ctgtttggat
    12601 gatggtttct tctgcatttt cctgtgtttc agacaggtca ataacctgac cgtcgatgat
    12661 cagttcgttt tccattgatt actcctggtt aactcggcat taagtctgcc ggtgactgtg
    12721 gtggtgactg gaattgctgt tgttgtgact cggcgacatc tttcagaagg cgtattgcct
    12781 ccatcactgc tttgtcatcg atgtttctgg cttgagccag tttatagaca gtgtttgcct
    12841 gactctccat cgcatcctgc tgggcagtaa atgctttgat ttgagtttga gcagtttcgt
    12901 tagttgcttt ttgcgcttct gcctgcgctg ctaccatttg cgcctgagcg agaaccattt
    12961 caggatttgg ctggctttgt gctgccattt gcgcctgttg aacaatctgc tgctctttct
    13021 cattgcgtgg ttttgcaata ccagatatca gcagttggtt tcggttgtac tctttgaagt
    13081 catcaaggcc ttcgccatcg atattgtcca gaataatacc ctgaattgcc gggcgcattg
    13141 ggtctgttgg aagcatagag ctaaggacat ttgtcagtac agaaaccgtt gcatcacgtc
    13201 gtgctgtgta gcttggtcca acatcaaccg tcacatcgta tcgaccgaca gaaaggtcat
    13261 ttaacgcaac aacagcccct gtttgcctgt caacaacctg tgcgctcagg accgcgatat
    13321 catcacttcc atcttcgtta acgatgcgca cttcacgctc tgaaccgtac acttcacgcg
    13381 ccattgacag ccatacttca ccagcgcgtt taagactttt cgccatattg tccagataga
    13441 taaacgaagc catatctgct ctgttcatca agttgttaac cgtttcctga gcaatattac
    13501 ttggcatctg ctgcatggcc tgactgccgc ctgtaacctc ctgaatatca gcactggttt
    13561 gctgtagtaa tgcagccaat gcctgattca taaccgcagg ctgtgtatat cctgccgggg
    13621 tagctccagc gataatgttg ccagatttat ctctcacttc gcgcaacggc aagaacgctg
    13681 ggcgtttctt gttgcgagcc tcccagtgct tctcaagtcc acgaatttgc tccatgccaa
    13741 ctatagggat ctgaccgggg tcttgcgctg cagtatcagc cagcattgat acctgaaggt
    13801 tatacaaacg ctgtggatcc attgcttttg caatatgtcc ttcgacacgc tcaatgtcat
    13861 caatgaacca gcgttttcca taaaccggga tgagggggat atgctcacca ggaatacgtc
    13921 gaggtttctc aaggaaacca tcaccatcca ctacggatac atacacacga cggcgcttca
    13981 ctgagcgcct tgccacttcc tgaaatccag ctattgccag ttcatcttca atatcttcaa
    14041 cctgatcact gtcgtatgtt gcaatctctc cagtgattgg atgtcgataa ctgatgacgc
    14101 caacagactc tttacgaact tcgtaatact tcgctatgta aataacatct tcatcaaacc
    14161 agtcatattc ccaactggtc atagacgtta catccagaga agcaggaggt ttctttccgt
    14221 attcagcctc atatttttca ggtgacaacg aatacatgca gaacgcccac aacgcgtcag
    14281 atttgtcgta cttcttagcg tcagggtcaa accacacaga gcgcgacggg tcgtatattg
    14341 gttcaatagc aatacgctga cgatcgtcca tgaggtcgta ttcattgacc agcatcgacg
    14401 tcaaacggaa gcaaccgaaa ccaccagtag cagcgtcgtc aaatgcatta tcgcaagcct
    14461 caccgccatc ggtttcttcg tagtcagcac ggaacagacc atttaattta ttggctaact
    14521 cttcgcttgc ctctctgtca ccaggacgaa acttaacagt gattctgtta ttgcggtatt
    14581 ctgcaatgat gcggttaagt tcagttgcta ccttattgat ttcaaactta ggatacttct
    14641 cgaactgctc atcaagctta gttccagccg ccgttgctcc ttcccattga cctcctggga
    14701 cacgagcaaa cctcgtagct tcaatgcact tttcgcgcac ttcctgctgt ggagaatagg
    14761 cgcggtcaaa cctgagcatg atccgctcat gttttttctc taatgtctct gccatgttta
    14821 ccaaccggag gatgagggaa cgtatatttc tgtttcttcg cggaccaatg ccgggcaatg
    14881 catacacatc atcagcgcat cagccaggtt aggagatggg ataccgagct tctgcttcat
    14941 ttcgacctta gtcataagct ccagcttccc gttattattg aatttgcgct gaatctgcgt
    15001 cagttctgca aacagcttct ccagcatctt ctcgcctatc gcttctttgt cgaagctcag
    15061 catgtcgtcg gggtctgcat actcaccgtg gacaaccgcc cgatatgtca gatacagcct
    15121 gtcagccagc gcgtaataga attgcgctcg cttattgcgg aatacatcgc caatagtgcg
    15181 aacgttgtcg ccctgtacga cttcatcagc ccatgctccg gcctgataag gcccatcttc
    15241 atcgaatggc gattcgctgc ccttgaacat cgtggcggta attttcttgc cggagaatgc
    15301 ttccgttgtc tgtctgcgta ggcccgcacc gacgccatca ccgtcccaca ggtaatggtc
    15361 agcgccgtct tcaatcgcca gcgaagtagc ccagtcagca ccctcgttga tgtccatcag
    15421 cagaccttcg gcaatgcgct taactaccga accgtgacgc gatgcataac ctttagcatc
    15481 tggccctgta tctgatgggt catgcgcaga aacaaccgca cctttcgctt tccacccgag
    15541 tttcgtgtgc gcatcggttg cggcttcaag ccattcacgt ttgatgattg ccatatcact
    15601 tgcgcttacc ggctcaccaa gccagatgtg acgatacagt gtcggatttc tgcgtttaca
    15661 ctcttccatc tccagacgga ggacttcagg aaaatgcggg ttgtcggtgt agttcaccgt
    15721 cagcaggcaa atatcatcgg gaggatttac gacgaatcgc tgataggtat cgtcgaggat
    15781 gtttttcggg ttgaaactta cccatatttc agagaacggc ttacggatgg ttggaatcag
    15841 gatatcccac gattccttcg ttaccgcttc cgcttcttcc acccagcaga tatcaatgcc
    15901 ttcgagcgat ttaatcttcg tcgggttgtt tttgatgccg tagaacatga attcagcatt
    15961 cgttccgaga tgacgaatca ttgaacgctg aatttcaaac tcagccgaat acccttcacg
    16021 ctcgatggtg tcttcaagca acctgattac cgaatcgctg atactgtttt gcagctcacg
    16081 agcgcaaaga atacgcacag gctgccgacg cgccgcttca acaagaagcc tcgcaattgc
    16141 ccatgactta ccgctacctc gaccgccttt ggcgactttg tagcgatgag cctcaatgaa
    16201 cggttcaaag ataggattaa tcgaggtcat tttccgaata gagtgctcat cggtgatgtt
    16261 tcaatctgga ttgcgccgcc gtctttgccg acaagctcgt tagttacctt gtcgccatac
    16321 ttacggggat tcattcgggc cagcgcccat ttgcgggtat caacgcgaag tcttgccttt
    16381 gccacctcgg cggcatcagg gattacgtcg tcagcaattt cgaatatctc ttcgaaaata
    16441 gagtcggccc gagtctctgt tgccttcgcg tactggtcac gaaactcctg atgttcagcc
    16501 agccagcgaa aaactgatgt tttgctcggc attcctgggc gttcgcaaac tttgcgcaga
    16561 ctctcaccgg aggaaagcaa tgcgcaaatg tcattagcca cctccggcat ataatcagag
    16621 gggcgaccac ctttcttttt ctcagtcgcc atattgatta tttcccttct gcttgcttat
    16681 cccattcatc gcggaatttg gatgggttgt cgaaaccttg agttgccatg tttacgctcc
    16741 ggtagtgaac aggtctaacg cttccttcga tttacgcacc gcttcgattg tgcgggtcgt
    16801 gatatctgaa ttagcgccac ctgactggaa gtgaattttg aatagctcaa gcttcagctc
    16861 gtcagtgcca atgaactgaa atgcttcctc cgcggctgcg ttctggttca tgaccagttt
    16921 gtaaatctct aactggaatt tctgttcttc agtcatggga ataatctctg ccattgttgg
    16981 ctccgtttat ccgttaaaag ggatatcagt taagttatcc cgtgtagggt ataagccatt
    17041 atcaaagcca ctcagtaagg aatggctttt gtaataacta ctgttcgctt agcttctgct
    17101 tcagcaagta accttcgagc atccagattt tgtttacagc attctgccgg gcaatcttcc
    17161 gaccaatttc tgcatcaaaa ttttcctgac ttgcacaggc actctctccg gtgacggtga
    17221 agccgttgcg cagcaccagg acgcagaacg tcagcagaga aagtgattcg tgcgactggt
    17281 agtttacctc tccgccagta tgtttcgctt ttatggcttt gccaaaggca ccatcctctg
    17341 ctgtgaaata tgcctcctga gcaataattc cttcgatatg gtctggcgta actcgcggtg
    17401 ccgttttgcc tttctcaacg atttcttttt cgatttgctg gtcgttcata attatgaccc
    17461 tgtagagtgg ttgcttgatt aggatgtctt tccatcagtt cgccaccaca aagaatcttt
    17521 tttgccataa ggctggaggt tcatctttca gtggctgcca gtgttatttc cccacttact
    17581 ggcttgggtt gtttcgcggt actgccgtaa ctggtggtgc acagatttag ttaaatctgt
    17641 tctcgcctga actatctttt acatacccgg attgtgggga tgtaaatcac ggtttcatta
    17701 tcaagcccac ccgtagatgg gctttggaat ggtcactttg gcagtccggg gatcgatatt
    17761 tgcgcctgct gctcaagcct tttgattctt gctatgagtt gcggtttttt gatcctgccc
    17821 cagcggttca gcaagcgtcc tgacatactg gcaacatcct tttccttcat gaactccagc
    17881 attaactcgt tgtgctctct ttggtatgag tgagccatct ccatcagcct gtcacgcatc
    17941 caattaaatg ctttgataaa cgcctctttg atggcggcag cttttttgcc ggtaaacgac
    18001 atgatgatgt acatcgcacc gtctttggaa atttcatatt caacatactg attacccttg
    18061 tgttcatagg taacccgcga aaagttgctg gttagaaatt catccgaaca gtctagcttt
    18121 tcgattttct gaatgatgtg gtgatgctgc ttgtcgaagt aagctgctac cttgcgggag
    18181 gttgtgatca cgcgatcacc agaaacaacc accatgtccc ggaaatcgag attagccaat
    18241 tgatgattca tagcgtcttt accttttaga aagtgagcct gtctcacaga aaagccgccc
    18301 cgagatggtc gccaccatat acggcagttc tcaggctcaa ctttctgaaa ggctcgggtg
    18361 atgtaatatg cgcgtgagat gcgctgtgaa attcagatgt aaaaaaagcc ccgcatcgcg
    18421 aggctcatta aatggacttt gtgatttgca aaaaaattat ttcaggcact gagtcctgat
    18481 gtactcctgc aggtagttaa cctgcgcggt tatcctgtcg attccacttc ggagacggta
    18541 ataattgagt tcagcatctg ctgtaagtct tgggctttct ccatcgccca tgctgctggc
    18601 tccggtcgtt gactttgcac aggtggcggc gacttgcagg cgcttacgac cagcagaaac
    18661 atcagcacga aggctttcga tagtagcgtt agcatcagca agctcctttg tgtatctggc
    18721 gtcgagttct gctacatcac gttgacgctt ctgcatatca gcgatgatgg atgtggcttt
    18781 atcgcgctgc tctttgtagg tcatggcgtt atcacggtaa tgattaacag cccatgacag
    18841 gcagacgatg atgcagataa ccagagcgga gataatcgcg gtgactctgc tcatacctca
    18901 atctctctga ccgttccgcc cgcttctttg aattttgcaa tcaggctgtc agccttatgc
    18961 tcgaactgac cataaccagc gcccggcagt gaagcccaga tattgctgca acggtcgatt
    19021 gcctgacgaa tatcaccgcg atcaatcatc ggcaaagcgc cacgctcttt aatctgctgc
    19081 agcgcaacag cgtcctggct tttgggagag aagtctttca ggccaagctg cttacgatag
    19141 gcatcccacc aacgggaaag aagctggtaa cgtccggcgg ctgttgattt gagttttggg
    19201 tttagcgtga caagtttgcg aggatgatcg gagtaatcag tgaatagctc tccgccaaca
    19261 atgacgtcat aaccatgatt tctggttttc tgccgtccat tatctgttcc ctctgaccac
    19321 gccagcatat cgaggaacgc cttgcgttga ttattgattt ccaccatctt ctactccggc
    19381 ttttttagca gcgaagcgtt tgataagcga accaatcgag tcagtaccga tgtagccgat
    19441 gaacacgctc gttatataag cgagattgct acttagtccg gcgaagtcga gaaggtcacg
    19501 aatgaaccag gcgataatgg cgcacatcgt tgcgtcgatt actgtttttg taaacgcacc
    19561 gccattatat ctgccgcgaa ggtacgccat tgcaaacgca aggattgccc cgatgccttg
    19621 ttcctttgcc gcgagaatgg cggctaacag gtcatgtttt tctggcatct tcatgtctta
    19681 cccccagaag gggatctgtt caaattagga attatggata tggtcgcttg aacaaatccg
    19741 ggttacggtt gatttgtaac gggtttgttc gtgaccgcat tcatgagcaa atcaggcgtg
    19801 gattgcgcca acaatacatg ccgctcatat cacgaagccc agccattgat gctgggtttt
    19861 cttttttaaa gcgtactaga caaccgtatc cacagagtgt cagcaatgag ttggttaggt
    19921 ctggttcttg gtggaagtac gctttaaaaa atgggctgat ggtgtagccc aaaatactga
    19981 gtgaatggta aggatgaaca acggttttgc tctgggtgga tttggctgtg gtggccggcg
    20041 ctgatctccg gcttgtatac aggcaccttg ttcttccgaa gctctcctgc gcgcatcagc
    20101 ctgcgcattc accacaccgg aaagagcact cagttgtacc ggccagttgt gccactaaga
    20161 aatgctttcg cagaccgtta agctctttgc cagttcttta atgctcttac ctgttgtgtg
    20221 cccattatta atcacaccgg gccagtgcgc cgaatttgtt tacaaggagt cggaagacct
    20281 tgctgactta caggctatta cgccgccatc agaacaacat catcgtttgc atttatcttt
    20341 gtggtcagtt tctaaaaaac cgcaaagtcg ccaactctga cgaaaactat cgttgtgctg
    20401 ccacaacgat aagagcactc ggtgcattta agccaagccc cataagggag aatgctctta
    20461 cctgttacac agatataaaa aatcccgaaa ccgttatgca ggctctaact attacctgcg
    20521 aactgtttcg ggattgcatt ttgcagacct ctcagcctgc gatggttgga gttccagacg
    20581 atacgtcgaa gtgaccaact gggcggaatc ggtagtaagc gccgcctctt tttatctcaa
    20641 taccacaacg agcgaattaa cccatcgttg ggtcaaattt acccaacttt attcaataag
    20701 tcaatatcat gccgttaata tgttgccatc cgtggcaatc atgctgctaa cgtgtgaccg
    20761 cgttcaaaat gttgtctgcg attgactctt ccttgtggca ttgcaccacc agagcgtcat
    20821 acagcggctt aacagtgcgt gaccaggtgg gttgagtaag gtttgggatt agcatcgtta
    20881 cagcgcgata tgcggcactt gctggcatcc ttgaatagcc gacgcctttg catcttccgc
    20941 actctttctc aacaactctc ccccactgct ctgtttttgc tatatcaacc gcacggcctg
    21001 taccgtgaca atctctgcat cttgcgcccg gcgtcgcggc actacggcaa taatccgcat
    21061 aagcgaatgt tgcgagcact tgcagtacct ttgccttagt atttccttca agctttgcca
    21121 cgccacggta tttccccgat accttgtgtg caaattgcat cagatagttg atagcctttt
    21181 gtttgtcgtt ctggctgagt tcatgcttac cgcagaatgc agccattccg aatccggctt
    21241 gtgattgcgc catccccata gcagccatca catcagtacc ggaaagagag tcagaagccg
    21301 tagcccgtgg tgagtcgctc atcatcgggc tttttggcga atgaaattta gctacgcttt
    21361 cgagtctcat gcgccttctc cctgtacatg aatcaatgtg aggtttccgc agaacactgc
    21421 gccggtatcg atatacatct ggttggcaaa cttgagtggt ttcactgctg gcgtatgacc
    21481 aaagatgaac atgtccgctc ctttgatttc tttcacgatc ccgtcttgtg agttgctgat
    21541 tcgttcgcgg ttccaaatta actgctgatg atcaactggc tttccaaact cgtattcgtc
    21601 acaaggataa tcggcgtggc agatgacata ttttttatct ttgctcacca gttcgatgat
    21661 taacggaagt tcacctgctt tatgggcaag agctttagcc agaatttctt tgtcgtaatc
    21721 gagattaaag aaccagccac cgccattaag cagccagtga ttgacgtttc cacgctctga
    21781 taagccatca atcatcattt gctcatggtt tccacgtaca gctctgaacc aggggaatgt
    21841 gattaattcc agacattcga cgttctctgt accgcgatcg accaaatcgc caaccgagat
    21901 aagcaggtct tttttggtgt cgaatcctat cgtctccagt tttttcatca ggttcgtgta
    21961 gcatccgtgc agatcgccaa ctacccaaat atttcggtat ttgctgccat caattctttc
    22021 gtaatagcgc atctctttca ctccatccgc gatgaaccat aagaacgtcg ttgacgatgg
    22081 cgtgcatttt cccgtcttta tcatcaacgt attttcttac cgtgccgcga ctacatttca
    22141 gtctgcgtgc tacttctgtc tggtttccgt atgcttcaac gagcatgtct ggaatggttt
    22201 ttactgagaa cgtcatgcgg cctcacttct gctatttcgc aggtctttga gtttctgttg
    22261 gtactctgcc ttgatcgcct tgcactcttc gatagtccag cgatggcggc tatggtttga
    22321 ttcgatttcg tctactgctt cctgccctat gcggttaatc agttcgacgc gatacggaac
    22381 gagatttccg cttttgtgct ggttgcacac cacgcattgc ttgtgaatat tgcgttcatc
    22441 aaatcggagt tgaggtgccg cagcagttgt ccggtaatgt ccggcatccc actgagcaga
    22501 cgtgagcgtt ccgcacgaga tacatggtaa gtcgcggtct ctttctctga tgaaggcgtt
    22561 tacagcttgt tgggcttgtt taatccagta actgcggggc tttaaggcga gttttcgaat
    22621 cttaagttta tctttctgtt tctgctcctc tcgtcgtcgt ttcttctctg ctgctttttc
    22681 cgctttttcg cgttctttac ttcgtcgttc gagtgctaat tgagttccgt gttccgggca
    22741 gcaccaccac tgatttgaga atgccgggtg aaaccattcc ttgcatattt tgcatttcct
    22801 tctcgctggt ttagccatcg tattcttcct cgtacattga gctattcgga tcgctcatca
    22861 gttctgcaca gcagt
//
LOCUS       DAZGOB010000071        22406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGOB010000071 DAZGOB010000000
VERSION     DAZGOB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.071"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..95
                     /locus_tag="ACHLIF_002964"
     CDS             <1..95
                     /locus_tag="ACHLIF_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO9606948.1"
                     /translation="FTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            74..178
                     /gene="speFL"
                     /locus_tag="ACHLIF_002965"
     CDS             74..178
                     /gene="speFL"
                     /locus_tag="ACHLIF_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO9606949.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            489..2687
                     /gene="speF"
                     /locus_tag="ACHLIF_002966"
     CDS             489..2687
                     /gene="speF"
                     /locus_tag="ACHLIF_002966"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO9606950.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2684..4003
                     /gene="potE"
                     /locus_tag="ACHLIF_002967"
     CDS             2684..4003
                     /gene="potE"
                     /locus_tag="ACHLIF_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO9606951.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4059..5699)
                     /gene="pgm"
                     /locus_tag="ACHLIF_002968"
     CDS             complement(4059..5699)
                     /gene="pgm"
                     /locus_tag="ACHLIF_002968"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO9606952.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGT
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5725..6270)
                     /gene="seqA"
                     /locus_tag="ACHLIF_002969"
     CDS             complement(5725..6270)
                     /gene="seqA"
                     /locus_tag="ACHLIF_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO9606953.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6455..7219
                     /gene="ybfF"
                     /locus_tag="ACHLIF_002970"
     CDS             6455..7219
                     /gene="ybfF"
                     /locus_tag="ACHLIF_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9606954.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7359..7652
                     /gene="ybfE"
                     /locus_tag="ACHLIF_002971"
     CDS             7359..7652
                     /gene="ybfE"
                     /locus_tag="ACHLIF_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO9606955.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7792..8322
                     /gene="fldA"
                     /locus_tag="ACHLIF_002972"
     CDS             7792..8322
                     /gene="fldA"
                     /locus_tag="ACHLIF_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO9606956.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8532..8618
                     /gene="uof"
                     /locus_tag="ACHLIF_002973"
     CDS             8532..8618
                     /gene="uof"
                     /locus_tag="ACHLIF_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO9606957.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8611..9057
                     /gene="fur"
                     /locus_tag="ACHLIF_002974"
     CDS             8611..9057
                     /gene="fur"
                     /locus_tag="ACHLIF_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO9606958.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9141..9467)
                     /gene="chiQ"
                     /locus_tag="ACHLIF_002975"
     CDS             complement(9141..9467)
                     /gene="chiQ"
                     /locus_tag="ACHLIF_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO9606959.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9517..10923)
                     /gene="chiP"
                     /locus_tag="ACHLIF_002976"
     CDS             complement(9517..10923)
                     /gene="chiP"
                     /locus_tag="ACHLIF_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO9606960.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11369..13033)
                     /gene="glnS"
                     /locus_tag="ACHLIF_002977"
     CDS             complement(11369..13033)
                     /gene="glnS"
                     /locus_tag="ACHLIF_002977"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO9606961.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13236..15182)
                     /gene="nagE"
                     /locus_tag="ACHLIF_002978"
     CDS             complement(13236..15182)
                     /gene="nagE"
                     /locus_tag="ACHLIF_002978"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO9606962.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15515..16315
                     /gene="nagB"
                     /locus_tag="ACHLIF_002979"
     CDS             15515..16315
                     /gene="nagB"
                     /locus_tag="ACHLIF_002979"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO9606963.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16375..17523
                     /gene="nagA"
                     /locus_tag="ACHLIF_002980"
     CDS             16375..17523
                     /gene="nagA"
                     /locus_tag="ACHLIF_002980"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO9606964.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17532..18752
                     /gene="nagC"
                     /locus_tag="ACHLIF_002981"
                     /gene_synonym="nagR"
     CDS             17532..18752
                     /gene="nagC"
                     /locus_tag="ACHLIF_002981"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO9606965.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18800..19552
                     /gene="nagD"
                     /locus_tag="ACHLIF_002982"
     CDS             18800..19552
                     /gene="nagD"
                     /locus_tag="ACHLIF_002982"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO9606966.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            19809..21473
                     /gene="asnB"
                     /locus_tag="ACHLIF_002983"
     CDS             19809..21473
                     /gene="asnB"
                     /locus_tag="ACHLIF_002983"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO9606967.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21854..21930
                     /locus_tag="ACHLIF_002984"
     tRNA            21854..21930
                     /locus_tag="ACHLIF_002984"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21888..21890,aa:Met,seq:cat)
     gene            21939..22023
                     /locus_tag="ACHLIF_002985"
     tRNA            21939..22023
                     /locus_tag="ACHLIF_002985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21973..21975,aa:Leu,seq:tag)
     gene            22047..22121
                     /locus_tag="ACHLIF_002986"
     tRNA            22047..22121
                     /locus_tag="ACHLIF_002986"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22079..22081,aa:Gln,seq:ttg)
     gene            22156..22230
                     /locus_tag="ACHLIF_002987"
     tRNA            22156..22230
                     /locus_tag="ACHLIF_002987"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22188..22190,aa:Gln,seq:ttg)
     gene            22246..22322
                     /locus_tag="ACHLIF_002988"
     tRNA            22246..22322
                     /locus_tag="ACHLIF_002988"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22280..22282,aa:Met,seq:cat)
BASE COUNT         5493 a         5652 c         5657 g         5604 t
ORIGIN      
        1 tcttcacaga ttatagccat ttcatggata gaataactct accttcaact gacacagcaa
       61 gaggtaaagg taaatggaaa ataataaccg cttaatgcct catataaggc ggacaaccca
      121 tatcatgaag tttgcccatc gtaatagttt tgactttcat ttctttaatg cccgctagtc
      181 ttctgactaa agggcacccc aacgtacagg tctccctgac tttaagcatt acaggttaat
      241 acctgtattc ctcggtgctc atatactgct aaccctttta aactctaaat aattcgagtc
      301 gcagcacttg caacttgagg tatgacgagt atagccagtt accgggctgg tctgggttat
      361 tgcatctgca aaaagcaaac tactgattta tttatcagcg gtggagcttt gctttttttc
      421 cggcgtgatc gatttctcct ttgagaaatt gaggacctgc tattacctga aataaagaga
      481 tgaacaaaat gtcagaatta aaaattgccg ttagtcgttc ttgcccggat tgtttttcca
      541 ctcatcgtgc atgcgtgaat atagacgaaa gtaattatat tgacgttgcc gccattattt
      601 tatcagtcag tgatgttgaa cgtggaaaac tcgatgaaat agacgctact ggctatgaca
      661 ttcctgtttt tattgcaacg gaaaatgaag aacgtgtacc cgcagaatat ctgccacgta
      721 tttctggtgt cttcgaacat tgcgaatcac gtaaagagtt ttatggacgt cagttagaaa
      781 ccgctgccag ccattatgaa acgcaactgc gcccgccctt cttccgcgca ctggtcgatt
      841 atgttaatca gggcaatagt gcttttgact gccctggtca tcaggggggt gagtttttcc
      901 gtcgccatcc ggcaggcaat cagttcgtgg aatactttgg cgaaatgctc ttccgctctg
      961 acttgtgtaa tgctgacgta gcaatgggcg atctgctgat tcatgaaggt gcgccatgta
     1021 ttgcacaaca gcatgcggct aaagtcttta atgccgataa aacctatttc gttttaaacg
     1081 gtacttcatc ttctaataaa gtggtcttaa acgcattgct gacgccgggc gatctggtgt
     1141 tgtttgaccg caataaccat aaatctaacc accacggtgc gttgctacag gctggcgcga
     1201 caccggttta tctggaaacc gcacgtaacc cgtatggctt tatcggtggc attgatgcgc
     1261 actgttttga agaaagttac ctgcgtgagc tgatcgcgga agtcgcaccg cagcgggcaa
     1321 aagaggctcg tcctttccgc ctcgctgtga ttcagttagg cacctacgac ggtacgattt
     1381 ataacgcccg ccaggtggtg gataaaattg gtcatctgtg tgactacatt ctgtttgact
     1441 ccgcatgggt tggctatgag caatttatcc cgatgatggc tgactgttcg ccgctgttgc
     1501 tggatcttaa tgagaacgat ccgggtattc tggttacgca atccgtacat aaacagcagg
     1561 ctggtttttc tcaaacttca caaatccata aaaaagacag ccacatcaaa gggcaacagc
     1621 gttatgtacc gcacaaacgc atgaacaacg cctttatgat gcatgcctcc accagcccgt
     1681 tctatccgct gtttgccgca ctggatatca acgccaaaat gcatgaaggt gtcagcggtc
     1741 gtaatatgtg gatggattgt gtggtaaatg gcattaatgc ccgcaagctg atcctcgata
     1801 actgtcagca tattcgtccg ttcgtacctg aactggtgga tggtaaaccc tggcagtcgt
     1861 atgaaacagc gcaaattgcg gttgatctgc gcttcttcca gtttgtacca ggtgagcact
     1921 ggcactcttt tgaaggctat gcagagaatc aatactttgt cgatccatgc aaactgttgc
     1981 tgacaacccc aggtattgat gcacgtaacg gcgaatatga agcgttcggt gtacccgcga
     2041 cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc gatcttaact
     2101 ccatcctctt cctgctgact ccggcagaag atatggccaa gcttcagcaa cttgttgccc
     2161 tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa gtgctgcctt
     2221 ccatctacaa acagcatgaa gagcgctacg ccggttatac cctgcgtcag ttgtgtcaag
     2281 aaatgcatga tttgtatgcc cgtcacaacg tgaaacaact gcaaaaagag atgttccgta
     2341 aggaacactt cccacgcgta agcatgaatc cgcaagaagc caactacgcc tatttacgcg
     2401 gtgaagtgga gctggttcgt ctgccggatg cagaaggccg tatcgctgcc gaaggtgcgc
     2461 ttccttatcc tccgggtgtg ctgtgtgttg ttccgggtga aatctggggt ggtgctgttt
     2521 tgcgttactt cagcgctctg gaagaaggga tcaacctgct gccaggtttt gcaccggagc
     2581 tgcagggtgt ctatatcgaa gaacatgatg gtcgtaagca agtttggtgc tatgtcatca
     2641 agcctcgtga tgcgcaaagc accctgttga aaggggaaaa attatgagtc aggctaaatc
     2701 gaacaaaatg ggcgtcgttc agttaaccat actgacgatg gtcaacatga tgggctccgg
     2761 tatcatcatg ctgccgacaa agcttgccga agttgggaca atttcaatta tctcctggct
     2821 ggtgacagcc gtaggctcaa tggcactggc atgggcattc gcaaaatgcg gtatgttcag
     2881 ccgtaaatca ggcggtatgg gcggttatgc ggagtatgct ttcggtaaat ccggtaactt
     2941 tatggcgaac tatacctacg gcgtctcgct gctgattgct aacgtcgcga ttgctatttc
     3001 ggcggttggt tacggcaccg aactgctcgg cgcgagtttg tcgccagtgc agattggtct
     3061 tgcgaccatc ggggtgctgt ggatttgtac cgtggctaac tttggtggtg cgcgcattac
     3121 cgggcaaatc agtagcatta ccgtgtgggg ggtcattatt ccggtggtag gcctgtgcat
     3181 tatcggctgg ttctggttta gcccgacgct ttacgttgat tcctggaatc cgcatcatgc
     3241 accgttcttc agtgcagtag gttcttccat cgctatgacg ctgtgggctt ttctcggtct
     3301 ggagtctgcg tgtgcgaata ctgatgtagt ggaaaacccg gaacgtaatg tgccaatcgc
     3361 ggtactcggc ggtacgttag gtgcggcggt gatttatatc gtctccacca acgtgattgc
     3421 agggattgtg cctaatatgg agctggcaaa ttcaacggca ccatttggcc tggccttcgc
     3481 acagatgttc acaccagaag tgggtaaagt cattatggcg ctgatggtga tgtcctgctg
     3541 cggttcgctg cttggctggc agttcaccat tgcccaggtg tttaaatctt catctgatga
     3601 aggctacttc cctaaaattt tctcccgtgt aaccaaagtg gatgcaccgg tgcagggaat
     3661 gttgaccatt gtgattatcc agagcgggtt ggcgctgatg acgattagcc cgtcgctaaa
     3721 cagtcagttc aacgtgctgg ttaacctggc cgtggtgacc aatatcattc cgtatattct
     3781 gtcaatggcg gcattagtca ttattcagaa ggtcgctaat gtgcccccct caaaagcgaa
     3841 agttgcaaac tttgttgctt ttgttggcgc gatgtatagc ttttatgcgc tgtactcatc
     3901 cggggaagaa gccatgctgt acggttccat cgtgaccttc cttggttgga cactgtatgg
     3961 tctggtatca ccacgctttg aactgaaaaa taaacacggt taaaaaagca aagtaaaaaa
     4021 gggcgatctt gcgaccgccc tttttttatt aaatgtgttt acgcgttttt cagaacttcg
     4081 ctaacaatct caaccgcttc tttctcaatc tgcttgcgat gttcttcacc gaggaagctt
     4141 tcgcagtaga tcttatatgc gtcttccgtg cctgacggac gcgcggcgaa ccagccgttg
     4201 tcagtcatca ctttcagacc gccgatagaa gcaccattac ccggtgccgc cgtcagacgt
     4261 gcggtgatcg ggtcacctgc cagggtgctg gcgctcacca tttccggaga cagcttagac
     4321 aacgccgctt tttgtgcgga agtcgcagcg gcctgcaaac ggttgtagct cggcgcacca
     4381 aagcgttttg ccagttcgtt gtagtgttcc tgcgggttct taccggtgac agcggtgatt
     4441 tccgccgcca gcagacacat aatgatgccg tctttatcgg tggaccacgg cgtgccgtcg
     4501 aaacgcagga aggaagcccc ggcgctctct tcgccgccaa agccgaaact gccgtcgaac
     4561 agaccatcaa caaaccattt gaatcctacc gggacttcta ccagtttacg gcccaaggcg
     4621 ttgaccacac ggtcgatcat cgcagaagaa accagcgttt taccgacagc aacatctttg
     4681 ccccactgcg gacgatgctg gaacaggtag ttgatcgcca ccgccaggta gtggttcgga
     4741 ttcatcaaac ctgccggagt gacgataccg tgacggtcat aatccgggtc gttagcaaac
     4801 gccagatcga acttatcacg cagtgccagc aggcccgcca tcgcacactc ggaggagcag
     4861 tccatacgga tcgtgccgtc tttatcaagg tgcataaagc ggaaggtttg atcgacctga
     4921 tcgttaacga tggtcaggtt gaggttgtaa tactcgccaa tacgcttcca gtattcgata
     4981 ccggaaccgc ccagcggatc aacgccaagc gtcaggcccg ctttctgaat cgccgccata
     5041 tcaacgatat cggccagacc ttccacaaac ggctgcacca gatcctgctc tttcacatga
     5101 ccggatgcca tcgcttcgtc gagagagata cgcttcacgc ctttcaggcc atcggccatc
     5161 agtgcgttgg ccctgtcttc caccactttg gtgacgttgg tatcagccgg gccaccattt
     5221 ggcgggttgt acttgatacc accatcttcc ggcgggttat gggacggtgt aatcacgata
     5281 ccgtctgcca gcgggccacc ttttttattg tgaaccagga tggcattgga aacggcaggc
     5341 gtcggagtga atccattgtt ttcctgcaca atgacatcaa cgccgttcgc tgccagcact
     5401 tccagtacgg aaatgaacgc aggctcggac agggcgtgag tatctttacc cacatagcaa
     5461 gggccagtga tgccattttt cgcacgttct tcagcaattg cctgagcgat cgccagaatg
     5521 tgcggctcgt taaagctgtg gcgcgctgca ctgccacggt gaccggaagt accgaatttc
     5581 accgcgtgct ccgcattccc tgcttctggt ttcagtacat aatattgcgc cgtcagttgg
     5641 gcgacgttaa tcaaatcact ctgttgtgca ggttggcctg cacgattgtg gattgccatt
     5701 gctttgtcct ttgtctgcaa cgttttagat agttccgcga accttctcaa tcaattccgc
     5761 cgggaattgc atcgactgca tgatgtgttc gatcatgctg catttacggc cggtgttggt
     5821 gttggtgatc acccaatacg gcgtgcctgg cacatgtttc ggcttggtct gattaccatt
     5881 tttcagcagc gtttgttcat ctgccgcaaa gtaaacgcgt gtacgaccgt gcaacgattc
     5941 cgttgcttcg gcaaacgcct gggcgtcaag agaatatagt gtagacaaca gcagcataaa
     6001 gcgattgacc gctcgctttt gctctgcgta ttcgtccgaa agcagaagtt cacgcattgc
     6061 gcgaaccttg tctttaatcg ttttgaccgg cttcgcttcg acgatagcag gtgacgcaac
     6121 gcgaacctct ttcgtcaccg gagcagcagg ctgtgatgag gcggaaaatt tcaacatacg
     6181 ccgtaaaatg tcggatgcgc tctcgccgat atgcttagtg tggctggcaa tatagctgta
     6241 gagttcatca tcaacttcaa tcgttttcat cttaatccag tgcagtgtct tagctgaata
     6301 caagtcgcca ggagtatatg aataaatccc ggcagcggat agcgtcaagc ctggcttgca
     6361 gaaaaactcg gaataaacct tatttgttgc agccctgtgg cacaatggtc aacataatac
     6421 cctaacccga caacgcgaaa aaaagaactt tgccatgaaa ttgaatatcc gcgcgcaaac
     6481 tgcacaaaac cagcacaata attctcccat cgttcttgtc catggtctgt ttggtagcct
     6541 cgacaacctt ggcgtactgg ctcgcgatct ggtaaacgat cacaatatca tccaggttga
     6601 tatgcgtaac cacggtcttt caccgagaga tccggtaatg aattacccgg cgatggcgca
     6661 ggatcttgtt gatactctgg atgcacagca gatcgacaaa gcaacattta tcggtcactc
     6721 catgggcggt aaagcggtaa tggcacttac tgcactggcc cccgatcgca tcgataaact
     6781 ggtggcgatc gatatcgcac cggtcgacta tcacgtacgc cgtcatgatg agattttcgc
     6841 ggctatcaac gcggtcagtg aatcggacgc acaaactcgc cagcaagcag cagcaataat
     6901 gcgccagcat cttaatgaag aaggggtgat tcagtttctg ttgaaatctt ttgttgacgg
     6961 ggagtggcgc tttaacgtgc cggtattgtg ggatcagtat ccgcatattg tgggttggga
     7021 gaaaatcccg gcatgggatc accctgccct gtttatccct ggcggcaatt ctccgtatgt
     7081 tagcgagcag taccgtgatg atttactggc acaatttcca caggcacggg cgcatgtgat
     7141 tgcaggcgcg ggtcactggg tccatgctga aaaaccggat gcggtattac gcgctatccg
     7201 tcgctatctc aatgattaac gcaactgatt aaaaacccag cgtccgcgtc gctttccgcg
     7261 cgtgggcgtt ggcgtgactc atcgcgctga tgtattatgg cgcgctatcc atccgggcag
     7321 aggcctggct gattgtttcc cccgaagtca ccaagatcat ggccaaagaa caaacggacc
     7381 gtacgacatt agatctgttc gcgcacgagc gtcgaccggg acgaccgaaa actaatccgc
     7441 tttcgcgcga tgaacagctg cgtattaata aacgcaacca gctaaaacgc gacaaagtac
     7501 gtggccttaa gcgtgtcgaa ctgaagctga acgcggaagc tgtcgaggcg ctgaacgagc
     7561 tggcggagtc gcgcaatatg agccgtagcg aactgatcga agagatgtta atgcagcaac
     7621 tggcggcgct gcgtagtcag ggcatcgttt aaatttccac tttcatgtag cacagtgtgc
     7681 agtcctgctc gtttgctgat tccgcaagac tgcctgttct gctatgattg cctttatccg
     7741 tgggcaattt tccaccccca tttcaataag tttcaagagg ttatttcact catggctatc
     7801 actggcatct ttttcggcag cgacaccggt aataccgaaa atatcgcaaa aatgattcaa
     7861 aaacagcttg gtaaagacgt tgccgatgtc catgacattg caaaaagcag caaagaagat
     7921 ctggaagctt atgacattct gctgctgggc atcccaacct ggtattacgg cgaagcgcag
     7981 tgtgactggg atgacttctt cccgactctc gaagagattg atttcaacgg caaactggtt
     8041 gcgctgtttg gttgtggtga ccaggaagat tacgccgaat atttctgcga cgcattgggc
     8101 accatccgcg acatcattga accgcgcggt gcaaccatcg ttggtcactg gccaactgcg
     8161 ggctatcatt tcgaagcatc aaaaggtctg gcagatgacg accactttgt cggtctggct
     8221 atcgacgaag accgtcagcc ggaactgacc gctgaacgtg tagaaaaatg ggttaaacag
     8281 atttctgaag agttgcatct cgacgaaatt cttaatgcct gatgtgatgc ggcgtagact
     8341 catgtctacg ccgtattaat agataatgcc aatcaaaata attgctacaa atttgtaact
     8401 tttgctgttg tacctgtaca atgtcccggt gttcaagtgg ccttgccgtt gtaaatgtaa
     8461 gctgtgccac gtttttatta acaatatttg ccagggactt gtggttttca tttaggcgtg
     8521 gcaattctat aatgatacgc attatctcaa gagcaaattc tgtcacttct tctaatgaag
     8581 tgaaccgctt agtaacagga cagattccgc atgactgata acaataccgc cctaaagaaa
     8641 gctggcctga aagtaacgct tccacgttta aaaatcctgg aagttcttca ggagccggac
     8701 aaccatcacg tcagtgcgga agatttatac aaacgtctga tcgatatggg tgaagaaatt
     8761 ggtctggcta cggtatatcg cgtactgaac cagtttgacg acgctggtat cgtcacccgc
     8821 cacaattttg aaggcggtaa atccgtattt gaactgacac agcaacatca ccacgatcac
     8881 ctgatttgcc tcgactgcgg caaggttatc gaatttagtg atgattccat cgaagcgcgt
     8941 cagcgtgaaa ttgccgcaaa acatggcatt cgcctgacta accacagtct ctatctttac
     9001 ggtcactgtg ccgaaggcga ttgccgcgaa gatgagcacg cgcacgaagg caaataagcc
     9061 agcctgaacg agaaaagcca acctgcgggt tggctttttt atacaaggga aataagaaaa
     9121 gcgagagtta cagctctcac ttatttgtta ttactgcgta tttcctgcca gaccttatca
     9181 caatcggctt tcaccgcctg atcatttccg gtttgcgttg cacgcagaca ctgctgataa
     9241 tccagtacgc gtacactttc ctggtcggca aactgctgat gttgtttctc tttcttcagc
     9301 acgtttaaca cgctctggca ggcttcaatt ttttctggcg aaccttgtgc ggtgttgata
     9361 caggcgctgt atgcctcttt cagacgactg tcttcctgtg gcgcggttga ttgcgcacaa
     9421 gctaccagcc ccgatgccat tatggcgatg agaatcagtt ttttcatagc aaattcctca
     9481 aacgtgcggc aggcacaaaa cctgccgcgt cgggcatcag aagatggtga atggtgcgat
     9541 taccataaat ttcacgtcac gctcatcctg gaagatgtta ccgtaaccgc cgccccagct
     9601 tgggatgtca gagtggttgt cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc
     9661 gcgaccgtcc tgaatggtgt agaccgcatc caggctgtag gcagactctt caatagtccg
     9721 gtttttgtcg tagtacgcat ccggattgct ctgccaggtc gcaggtttag catcccatgc
     9781 gtaaacgtag gaagcgccga tggcgaagcc tggaagattc cagtttttca ggtcatacat
     9841 cgcaccgaag aagaccgctt tttcgccgtt ggcgttgaag tcggaacggt tatcccacca
     9901 gatatccagg cggccgttgg aggaagcgta ggttggagtc atacgttgca ggaagtatcc
     9961 ctgctgaccg tcagccttaa cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc
    10021 ccggtaacca aaggtcaacg cttgcagcca ggcggtgccg tcataaaggt cgttgacgct
    10081 gcgatcgtca actttatcgc gcgtaccata gaactggtag ctggtggtta acgggctacc
    10141 ggcgatatca aatttgtagc tggctttggc aaaatattga tcgatatacc cttccgcctg
    10201 accaaatgcc gcttccagta cgaagttatt tttgaagtcg tatttcgcac cgatggagtg
    10261 caggtaatca actttggtgg ttttatcgtt ctgataaaac tcatccattt ccagatgcca
    10321 cggcgctttg tattcgttgg tccacatgta ggagaaactc aaggcaccag catcgccgta
    10381 atcaaaattc gccccggctt ccgccccctg ataagtacct ggcataaagc tccagtgcgg
    10441 cgctaacagc gtttgaccag ttggctgaat gtaacctgcc ctcgcccaaa ccggaccgta
    10501 tttaaatttg gccgcagctt tatacaggct tataccgctt ttgtcgccgg accagtcttc
    10561 gtcataggct ttattacttt ttgaaaacgc gatttcgttc gggtgggagc tgtcgccgtt
    10621 ttccgccatt tcaatcgccg taaacgcagc aatatcaagg ccgaacatat cagcagcata
    10681 accggactga aaatcgaggt tggcattcca ggtggagtga gaaaggttgg ttttgtattt
    10741 gtcgccgtcg gtaacatctt tacggtcgcg ttcacgctgc cagtaatata taccgccggt
    10801 taaggttgaa tcgtcgatga atccttcggc gcgagcctcc ggcattgcca taaagcccga
    10861 cattgctgta acaccggcga tagccagcgc cagcgtacta cgtttgccac taaacgtacg
    10921 catgggttaa tcctctttga cgtataaatt gctgcaccaa aggtgaacag cgaaaaaata
    10981 aaaattaaaa aagtagcgtt gagaaaagtc tcgctgacta caggaacaga ctatttttcg
    11041 cgacgcgaat tatcaatctc tttctgtaac cagaatgtaa gaacatgagc aaccgcacat
    11101 attttgatga tttttgttac aaggcttaag tttgcggttg aaaggcttgc agaagaataa
    11161 aagcgtgaat ttccagcaag atgaaagcgg ttacatttta tttaattatg gtttgtgatt
    11221 ttaaatttat tttatggata aattgcgaag cggaattaat tcgcgaagaa aagcagaaaa
    11281 aacgccgcag agtgcggcgt ttgggattgt ttgctatttc ttgccggatg cgacgtaaat
    11341 gcctcacccg gcctggtaga acttaaaatt actcgcctac tttcgcccag gtatcacgca
    11401 gcccaacggt gcggttaaat accggttttt ccgctgtgga atggcggctg tcgaggcaga
    11461 agtaaccttc acgctcaaac tggaatgctt tacccgcaac cgcatctttc agcgacggtt
    11521 cagcaaagcc ctgtttgatc accagcgatt ccgggttaat caccgacagg aaatcatccg
    11581 cagcaccagg gttcggcacg ctgaacagac ggtcgtacag acggatttca accggcagcg
    11641 catgtgccgc gctcacccag tgaataacac ctttgacttt acgaccatct gccggatctt
    11701 tgcttaaggt gtcggcgtca taagtacaga agatggtggt gatattgcct tcggcatctt
    11761 tctccacgcg ttcagcctta atgacgtaag cattacgcag acgcacttct ttacccagca
    11821 ccagacgttt gtactgcttg ttagcttctt cgcggaaatc ggcgcgatcg atccaaatct
    11881 caccgctaaa cggcacctga cgactcccca tttccggttt gttcggatgg ttcggcatgg
    11941 tgaccatttc gccttcgccc tgatagtttt cgataaccag tttcaccgga tcgataaccg
    12001 ccattgcgcg cggcgcattt tcgttgagat cttcacggat gcaggattcc agcgatgcca
    12061 tctcaatggt gttgtcctgc ttggtcacgc cgatgcgttt gcagaactca cgaatagaag
    12121 cagcagtgta accacgacga cgcagaccgg aaatggtcgg catacgcggg tcatcccagc
    12181 cttcaacgtg cttgtcggtc accagcaggt tcagcttacg cttggacatc acagtgtatt
    12241 ccagattcag gcgcgagaat tcgtactggc gcgggtgaac aggaatggtg atgttgtcca
    12301 gcacccagtc gtacaggcga cggttgtcct ggaactcaag cgtacacaga gagtgcgtaa
    12361 taccttccag ggcatcgctg atgcagtggg tgaagtcgta catcgggtag atgcaccact
    12421 tgttgccagt ctggtggtgt tcagcaaatt taatacggta cagcaccgga tcgcgcatca
    12481 cgataaacgg tgaagccatg tcgattttcg cacgcaggca ggctttacct tcttcaaaac
    12541 caccggcacg cattttttcg aacagcgcca ggttctcttc aacgctgcgg tcgcggtacg
    12601 ggctgttttt acccggttgc gtcagggtgc cgcggtattc gcggatctgt tccggcgtca
    12661 gttcatcaac gtacgccagg cctttattga tcagttcgat cgcataggcg tggagctgat
    12721 caaaataatc ggaggagtaa cggacgttac cagaccagtg aaaacctaac cactctacgt
    12781 cgtttttgat cgactcaaca tactcgatat cttcttttac cgggttagtg tcgtcgaaac
    12841 gcaggttgca ctggccttta tagtcctggg cgatcccgaa gttcaggcag atagatttcg
    12901 catggccaat atgcagatag ccattcggct ccggcgggaa acgggtgtgt actgtggtgt
    12961 gcttaccact ggccagatct tcatcgatga tctgacggat aaagttagtc gggcgggctt
    13021 ctgcctcact catcgtggat tcctcaaagc gtaaacaacg tataacggcg tatgatctta
    13081 taagcgggac aggctgacaa ctgttagttt tttaaaattg ataatctaac aaggattatg
    13141 gggatgattg atgaaaaaaa aagcggcaga gaagatctcc accgcttgtc attgttggat
    13201 gcgacgctca agcgtcgcat caggcataaa gcagattact ttttgatttc atacagcggt
    13261 gtttgacccg ccacaacatg gccctgagct ttaatgatca agccactgaa atcgtcgata
    13321 ttgctgcaaa ccaccgggct aatcatcgag cgggcgttag cgttcaggta atccagatcc
    13381 atttccagaa tcggttgccc tgcgcttacc tgcgcaccct cttccaccag acgtttaaag
    13441 cctttacctt ccagcgctac ggtgtcgata cccatatgga cgacgatctc cgcgcctttt
    13501 tcggtttcca ggcagaacgc gtggttggtg ttgaagattt tcacgattgt ccctgcggct
    13561 ggtgatacga cgattttatc tgtcggtttc accgccacac cgtcacccac cgctttgctg
    13621 gcgaatgctt cgtcaggaac ctgatccagt gccacgacat caccggtaat cggcgatacc
    13681 agctccgcga tagataccgc gtttggtaca gcctgcggtt ttgctacagg cgcggcagtt
    13741 gccggagttg cttcagctga cgcagcggct accggaccac gggcaacgac tttcttcatc
    13801 gcatcgccga tggattctgc tttcgcgcca acaatcacct gaatagtctg tttgttcagt
    13861 ttcactacac cagaagcacc cagacgttta cacatcgtat cgttgacgcg ggcagagtca
    13921 gccacggtca ggcgcagacg ggtgatacag gcgtcaattg ctttcaggtt gtcagtgccg
    13981 ccaaccgcag caatatagtt ggtcgccagt tgattgaggc cttcttcagt gttgctgttg
    14041 gcttcttcag taacgatctc gtcttcttta tcttcacgac ccggcgtttt caggttgaac
    14101 atgcggataa ccaaactgaa caccacgaag tagatagcga agaagacaac acccataacc
    14161 agcagcatcc agacgttctg gctggcggcc ggcaggttat acatcaacgc gtagtcgata
    14221 gcccccgctg agaaagagaa gccagcatgg atacccagca gtgttgccac aaacaggctg
    14281 ataccggtca gcagtgcgtg caggaggtac agcagcggtg caaggaacat gaacaggaat
    14341 tccagcggct cagtcacacc ggtcaggaac gcagtaacag caacagaaag cagcatcccg
    14401 ccaaccatcg gacgacgctc tttcggtgct gcgaagtaca tcgccagcgc cgcacccggc
    14461 agaccgaaca tcataatcgg gaagaagccg gacatgaaca tccccgcggt gccgtcaccg
    14521 gcatagaagc ggttgatatc accgtggaaa accgtacccg ccgcgttggt gaattcacca
    14581 atctggaacc aggcgatggt gttcagcacc tgatgcagac cggttgggat cagcagacgg
    14641 ttgatgaaac caaagatacc ggaacccagc gcgcccgcag aaacgatcca ctcgcctcct
    14701 gcatggatag cgtgctgtac cggcggccag acgtaaccaa aaatggccgc cagcaccagg
    14761 cagaagaagc cggtggcaat cggcacaaag cgtttgccgc cgaagaaact caggaagtcc
    14821 ggcagtttaa tatcggacca acggttatag gctgcgccac caaccagacc ggtaatgata
    14881 cccgccagta cacccatgtt aatttctggg ttgatggtca ccatcgcttt ggttaacaca
    14941 aagtaaccta ccgcacccgc cagcgccgcc gcacctgcgc tgtctttcga ccagctggat
    15001 gccacaccga tggcgaagat taatgcgagg ttatcaaaaa tcgcaccgcc cgcctgggca
    15061 ataaacgcaa cgttaagtaa atctggctga ccgaatcgca gcaacagtgc cgccaccggc
    15121 agcaccgcga tagggagctg taacgcccta ccgagtcgct ggaaaaaacc taaaatattc
    15181 atcttattcc ccctacgaga accctatttg gctcgtttaa agccgtattt ttattttgct
    15241 gcaaattgta ctgccgatgt tctgtaatca gattgttaga tcatctgcta cagagtgtgt
    15301 gaaaatttaa ttcgtatcgc aaattaaacg cgtgtctttt gtgagttttg tcaccaaata
    15361 tcgttatcat cactcccttt tactggctaa agcagaaaac ttattttatc attcaaaaaa
    15421 tcaggtcgga ttgacgcctg tctgcgcaaa tccaggttac gcttaaagat gcctaatccg
    15481 ccaacggctt acattttact tattgaggtg aataatgaga ctgatccccc tgactaccgc
    15541 tgaacaggtc ggcaaatggg ctgctcgcca tatcgtcaat cgtatcaatg cgttcaaacc
    15601 gactgccgat cgtccgtttg tactgggcct gccgactggc ggcacgccga tgaccaccta
    15661 taaagcgtta gtcgaaatgc ataaagcagg ccaggtcagc tttaagcacg ttgtcacctt
    15721 caacatggac gaatatgtcg gtctgccgaa agagcatccg gaaagctact acagctttat
    15781 gcaccgtaat ttcttcgatc acgttgatat tccagcagaa aacatcaacc ttctcaacgg
    15841 caatgccccg gatatcgacg ccgagtgccg ccagtatgaa gaaaaaatcc gttcttacgg
    15901 aaaaattcat ctgtttatgg gcggtgtagg taacgacggt catattgcat ttaacgaacc
    15961 ggcgtcttct ctggcttctc gtactcgtat caaaaccctg actcatgaca ctcgcgtcgc
    16021 gaactctcgt ttctttgata acgatgtcaa tcaggtgcca aaatatgccc tgactgtcgg
    16081 tgttggtaca ttgctggatg ccgaagaagt gatgattctg gtgctgggta gccagaaagc
    16141 actggcactg caggctgcag ttgaaggttg cgtgaaccac atgtggacca tcagctgtct
    16201 gcaactgcat ccaaaagcga tcatggtgtg cgatgaacct tccaccatgg agctgaaagt
    16261 taagacttta agatatttca atgaattaga agcagaaaat atcaaaggtc tgtaattgtt
    16321 atccctgccc tggctccttg ctcagggcaa tattttttaa aatcgggggt cagaatgtat
    16381 gcattaaccc agggccggat ctttaccggc cacgaatttc ttgatgacca cgcggttgtt
    16441 atcgctgatg gcctgattaa aagcgtctgt ccggtagcgg aactgccgcc agagatcgaa
    16501 caacgttcac tgaacggggc cattctctcc cccggtttta tcgatgtgca gttaaacggc
    16561 tgcggcggcg tacagtttaa cgacaccgct gaagcggtca gcgtggaaac gctggaaatc
    16621 atgcagaaag ccaatgagaa atcaggctgt actaactatc tgccgacgct tatcaccacc
    16681 agcgatgagc tgatgaaaca gggcgtgcgc gttatgcgcg agtacctggc aaaacatccg
    16741 aatcaggcgt taggtctgca tctggaaggt ccgtggctga atctggtaaa aaaaggcacc
    16801 cataatccga attttgtgcg taagcctgat gccgcgctgg tcgatttcct gtgtgaaaac
    16861 gccgacgtca ttaccaaagt gaccctggca ccggaaatgg ttcctgcgga agtcatcagc
    16921 aaactggcaa atgccgggat tgtggtttct gccggtcact ccaacgcgac gttgaaagaa
    16981 gcaaaagccg gtttccgcgc ggggattacc tttgccaccc atctgtacaa cgcgatgccg
    17041 tatattaccg gtcgtgaacc gggcctggcg ggcgcgatcc tcgacgaagc tgacatttat
    17101 tgcggtatta ttgctgatgg cctgcatgtt gattacgcca acattcgtaa cgctaaacgt
    17161 ctgaaaggcg acaaactgtg tctggttacc gacgccaccg cgccagcagg tgccaacatt
    17221 gaacagttca tttttgcggg taaaacaata tactaccgta acggactttg tgtggatgag
    17281 aacggtacgt taagcggttc atccttaacc atgattgaag gcgtgcgtaa tctggtcgaa
    17341 cattgcggta tcgcactgga tgaagtgctg cgtatggcga cgctctatcc ggcgcgtgcg
    17401 attggcgttg agaaacgtct cggcacactc gccgcaggta aagtagccaa cctgaccgca
    17461 ttcacacctg attttaaaat caccaagacc atcgttaacg gtaacgaggt cgtaactcaa
    17521 taagagaaag tatgacacca ggcggacaag ctcagatagg taatgttgat ctcgtaaaac
    17581 agcttaacag cgcggcggtt tatcgcctga ttgaccagta cgggccaatc tcgcggattc
    17641 agattgccga gcaaagccag cttgcccccg ccagcgtaac caaaattacg cgtcagctta
    17701 tcgaacgcgg gctgatcaaa gaagttgatc agcaggcctc caccgggggc cgccgcgcta
    17761 tctccatcgt caccgaaacc cgcaatttcc acgcaatcgg cgtacggctt ggtcgtcacg
    17821 acgccaccat caccctgttt gatctcagca gcaaagtgct ggcagaagaa cattacccgc
    17881 tgccggaacg tacccagcaa acgctggaac atgccctgtt gaatgccatt gctcagttta
    17941 ttgatagcta ccagcgcaaa ctgcgcgagc tgatcgcgat ttcggtgatc ctgccagggc
    18001 ttgttgaccc ggacagcggc aaaattcatt acatgccgca tattcaggta gaaaactggg
    18061 ggctggtaga agcactggaa gaacgtttta aagtgacctg tttcgttggt cacgatatcc
    18121 gtagtctggc gctggcggag cactacttcg gtgcaagtca ggattgcgaa gactccattc
    18181 tggtgcgtgt ccatcgcgga accggggccg ggattatctc taacgggcgc atttttattg
    18241 gccgcaacgg caacgtcggt gaaattggcc atattcaggt cgaaccgctg ggtgaacgct
    18301 gccactgcgg caactttggc tgcctggaaa ctatcgctgc caacgccgct attgaacaac
    18361 gggtgttgaa tctgttaaag cagggctacc agagccgcgt gccgctggac gactgcacca
    18421 tcaaaactat ctgcaaagcc gcgaacaaag gcgatagcct ggcctcggaa gtgattgagt
    18481 atgtcggtcg tcatctgggc aaaaccatcg ccattgctat caacttattt aatccgcaaa
    18541 aaattgttat tgccggtgaa atcaccgaag ccgataaagt gctgctccct gctattgaaa
    18601 gctgcattaa tacccaggcg ctgaaggcgt ttcgcactaa tctgccggtg gtacgttctg
    18661 agctggatca ccgctcggca atcggcgctt ttgcgctggt aaaacgcgcc atgctcaacg
    18721 gtattttgct ccagcatttg ctggaaaatt aatgtgcttt tatagtggcg cttattgttg
    18781 tcaatattct gggtagtcca tgaccattaa aaatgtaatt tgcgatatcg acggcgtgct
    18841 gatgcacgat aacgtcgccg taccgggtgc agcggaattt ttgcacggga ttatggataa
    18901 aggcctgccg ctggtgttgc tgaccaacta tccttcgcag accgggcaag atctggcgaa
    18961 ccgctttgcc actgccggtg tcgatgtgcc ggatagcgtg ttttatacct ctgcgatggc
    19021 gactgccgat ttcctgcgtc gccaggaagg caagaaagcg tatgtggtgg gcgaaggcgc
    19081 actgattcat gaactgtaca aagccggttt cactattacc gatgtgaacc ctgattttgt
    19141 gattgttggc gaaacgcgtt cctacaactg ggacatgatg cataaagcgg cctatttcgt
    19201 cgctaacggt gcacgcttta tcgccaccaa cccggacacc cacgggcgcg gtttttatcc
    19261 cgcttgtggc gcgttgtgtg cagggattga gaaaatctcc gggcgcaaac cgttctatgt
    19321 tggtaagccc agcccgtgga tcatccgcgc ggcattaaac aaaatgcagg cgcattcgga
    19381 agaaacggtg attgtcggcg ataacttgcg caccgacatt ctggcgggct tccaggcggg
    19441 tctggagacg attctggtgc tttctggtgt ttcatcgctc gacgatatcg acagtatgcc
    19501 gttccgccca agctggattt acccgtcggt cgctgaaatc gacgttatct gaatagcaac
    19561 cacgtctcag ggcgtggttt cttattttta cccatcatta aaatcatccc attcaataaa
    19621 actcgccaaa aattatcgat ccatcaataa atacatcacc acaccctccc ctttttcaca
    19681 aatcagcaat caccattacg ttttttattt ttgcactgcc aaatcgcttg cgcacggtgc
    19741 tgctttgcgg cattttttta aacaagcaaa cacaacaagc aacaaatacc aggttaacgg
    19801 agaaggttat gtgttcaatt tttggcgtat tcgatatcaa aacagacgca gttgagctgc
    19861 gtaagaaagc actcgagctg tcacgcctga tgcgtcatcg tggcccggac tggtccggta
    19921 tttatgccag cgataacgcc attctcgccc acgaacgtct gtcaattgtt gacgttaacg
    19981 cgggggcgca acctctctac aaccaacaaa aaactcatgt gctggcggta aacggtgaaa
    20041 tctacaacca ccaggcactg cgcgccgaat atggcgatcg ttatcagttc cagaccggat
    20101 ctgactgcga agtgatcctc gcgctgtatc aggaaaaagg gccggaattt ctcgacgact
    20161 tgcagggcat gtttgccttt gccctgtacg acagcgaaaa agatgcatat ctgattggtc
    20221 gcgaccatct ggggatcatc ccactgtata tgggctatga cgagcacggt cagctgtatg
    20281 tggcctcaga aatgaaagcc ctggtgccag tttgccgcac gattaaagag ttcccggcgg
    20341 ggagctattt gtggagtcag gacggcgaaa tccgttctta ttatcatcgc gactggttcg
    20401 actttgatgc agtgaaagat aacgtgaccg acaaaaacga gctgcgtcag gcactggaag
    20461 attccgttaa aagccatctg atgtctgacg ttccttacgg tgtgctgctc tcgggtggtc
    20521 tggattcctc aattatttcc gcgatcacca agaaatacgc cgcccgtcgc gtggaagatc
    20581 aggaacgctc tgaagcctgg tggccgcaat tacactcctt tgctgtaggt ctgccgggtt
    20641 caccggatct gaaagcagcc caggaagtgg caaaccatct gggcacggtg catcacgaaa
    20701 ttcacttcac tgtacaggaa ggtctggatg ccatccgcga cgtgatttac cacatcgaaa
    20761 cctatgatgt gacaactatt cgcgcttcaa caccgatgta tttaatgtcg cgtaagatca
    20821 aggcaatggg cattaaaatg gtgctgtccg gtgaaggttc tgatgaagtg tttggcggtt
    20881 atctttactt ccacaaagca ccgaatgcca aagaactgca tgaagagacg gtgcgtaaac
    20941 tgctggccct gcatatgtat gactgcgcgc gcgccaacaa agcgatgtca gcctggggcg
    21001 tggaagcacg cgttccgttc ctcgacaaaa aattccttga tgtggcgatg cgcattaacc
    21061 cgcaggataa aatgtgcggt aacggcaaaa tggaaaaaca catcctgcgt gaatgttttg
    21121 agtcatacct gcccgcaagc gtggcctggc ggcagaaaga gcagttctcc gatggcgtcg
    21181 gttacagttg gatcgacacc ctgaaagaag tggcggcgca gcaggtttct gatcagcaac
    21241 tggaaactgc ccgcttccgc ttcccgtaca acacgccaac ctcaaaagaa gcgtatctgt
    21301 accgggagat ctttgaagaa ctgttcccgc ttccgagcgc cgctgagtgc gtgcctggcg
    21361 gtccttccgt cgcgtgttct tccgctaaag cgattgagtg ggatgaggcg ttcaagaaaa
    21421 tggacgatcc gtctggtcgt gcggttggcg ttcaccagtc ggcgtataag taagaaatgc
    21481 gaaacagccc cggtgaaatc cggggctgtt tcagttattg aggtgcttgt taattctgat
    21541 atgcagtaag gaaatgttcc gggtttgcca catcactgtt tgacaactca tcactgtagg
    21601 ccagtatttc atagattaat ttcccaccac tattgttgaa ttccgaattt catcctgtac
    21661 gacaaaagaa tggctgtagg gtggggaaaa aacacaaagt aaacaataat tgacgaatat
    21721 agcgccacgc tgttcgcaac ctaaccaaac agtcactttc gagcaatttt ccttgaaaaa
    21781 gaggttgacg ctgcaaggct ctatacgcat aatgcgcccc gcaacgccga taaggtatcg
    21841 cgaaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    21901 tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag
    21961 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    22021 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    22081 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    22141 tcaccgcccg gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    22201 tccctggttc gaatccaggt accccagcca tcgaagaaac aatctggcta cgtagctcag
    22261 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    22321 caaattctga atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg
    22381 ccaagcggta aggcaccgga ttctga
//
LOCUS       DAZGOB010000072        22249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGOB010000072 DAZGOB010000000
VERSION     DAZGOB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.072"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(43..390)
                     /gene="trbJ"
                     /locus_tag="ACHLIF_002989"
     CDS             complement(43..390)
                     /gene="trbJ"
                     /locus_tag="ACHLIF_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO9606968.1"
                     /translation="MPPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVISLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTGLNELS
                     EISEETYNERKKK"
     gene            complement(320..865)
                     /gene="trbB"
                     /locus_tag="ACHLIF_002990"
     CDS             complement(320..865)
                     /gene="trbB"
                     /locus_tag="ACHLIF_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO9606969.1"
                     /translation="MSLTKSLLFTLLLSAAAVQASTRDEIERLWNPQGMAAQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(852..1133)
                     /gene="traQ"
                     /locus_tag="ACHLIF_002991"
     CDS             complement(852..1133)
                     /gene="traQ"
                     /locus_tag="ACHLIF_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001732192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO9606970.1"
                     /translation="MSKYKFSLPRLDITGVWVFSLGLWFHIVARLVYRKPWMAFFLAE
                     LIAAILVLFGAYQILDTWIARVSREEREALEARQAAILAAEKESRHVSH"
     gene            complement(1200..1601)
                     /locus_tag="ACHLIF_002992"
     CDS             complement(1200..1601)
                     /locus_tag="ACHLIF_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606971.1"
                     /translation="MDKENSSVSKRWLITSGVAVILLTLLAFMSFPDPDVSMNRYYSW
                     LMNVLILSVTGVMYVALSVVMVMLYFLYERNSRLLYLSVLALIIAAGIWLLRFLDNFL
                     MMPAFHVALFVLWVFLCFLYIRCKNEMSISE"
     gene            complement(1732..2094)
                     /locus_tag="ACHLIF_002993"
     CDS             complement(1732..2094)
                     /locus_tag="ACHLIF_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606972.1"
                     /translation="MNFISLWPLLLAQVTVMSLTVIFLAADRRFFSWDCFVVCTLSMM
                     MAEGIVIMKYQLWHIPPALLGLAGVLSWMLLLLHTGYFSGMSVVHKRLWLAVASVFSG
                     AGMIIFYWLLQPSETIMK"
     gene            complement(2108..2851)
                     /gene="traF"
                     /locus_tag="ACHLIF_002994"
     CDS             complement(2108..2851)
                     /gene="traF"
                     /locus_tag="ACHLIF_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO9606973.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKMKQPEPENKPAPVI
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQTQQRQAIARLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(2844..3101)
                     /locus_tag="ACHLIF_002995"
     CDS             complement(2844..3101)
                     /locus_tag="ACHLIF_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO9606974.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKESTAGGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERKNDERNEDE"
     gene            complement(3128..4978)
                     /gene="traN"
                     /locus_tag="ACHLIF_002996"
     CDS             complement(3128..4978)
                     /gene="traN"
                     /locus_tag="ACHLIF_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO9606975.1"
                     /translation="MKRILLLILPLVACMAQADRNSDYRAGSDFARQIQGQGTGSIQN
                     FKPQESIPGYNASPDETKYYGGVTAGGDGGLKNNGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEFCTRT
                     ARITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGAVESASLSVYAAFFFL
                     NSRYTFMNSTFNVGLVNGQSGTFPLSGATGLQVTQGQVLTGSGCTANGNCLSGGNGDR
                     QVYEKLASGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLKGTECSEPGGTKT
                     GVMEGKPWSITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKSPDCRGIT
                     VDELQRIKFDQLDFTNFYEDLMNNQKIPDSGVLTQKVKEQITDQLKQAGQ"
     gene            complement(4975..5613)
                     /gene="trbC"
                     /locus_tag="ACHLIF_002997"
     CDS             complement(4975..5613)
                     /gene="trbC"
                     /locus_tag="ACHLIF_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO9606976.1"
                     /translation="MRRKIVVLFALTAGLYISAHASENMNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRENPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKATAEAVLALVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVASAGDCRQVAHDLLAGKGDSGK"
     gene            complement(5741..6073)
                     /locus_tag="ACHLIF_002998"
     CDS             complement(5741..6073)
                     /locus_tag="ACHLIF_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606977.1"
                     /translation="MEKKLSSKYIHIYMSISLVLIRLISKTLKLFPFALVMVFTVLFY
                     FIPENVISDIISEWQKSDVSDRLRIIRLCLEITFLCALTAVFFEFLCSDEFLPRKKVN
                     QNEEVNNR"
     gene            complement(6100..7092)
                     /gene="traU"
                     /locus_tag="ACHLIF_002999"
     CDS             complement(6100..7092)
                     /gene="traU"
                     /locus_tag="ACHLIF_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO9606978.1"
                     /translation="MKRCLWLCVVFLLAGHIPVVSADAACEGRFVNPITDICWTCIFP
                     LSLGSTKVSQGKVPDTANPSMPIQFCPAPPPIFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGMTIPALGKTGQGTAKKDDRQVNGAFYHVHWYKYPVTYWLNIITSAGCLMGGDMD
                     IGYISEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNTIGQNTAVCYEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(7089..7721)
                     /gene="traW"
                     /locus_tag="ACHLIF_003000"
     CDS             complement(7089..7721)
                     /gene="traW"
                     /locus_tag="ACHLIF_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO9606979.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRIYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEDRK"
     gene            complement(7718..8104)
                     /gene="trbI"
                     /locus_tag="ACHLIF_003001"
     CDS             complement(7718..8104)
                     /gene="trbI"
                     /locus_tag="ACHLIF_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO9606980.1"
                     /translation="MTTTQKTTDVTAERRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDSASQKQLSEVQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(8101..10731)
                     /gene="traC"
                     /locus_tag="ACHLIF_003002"
     CDS             complement(8101..10731)
                     /gene="traC"
                     /locus_tag="ACHLIF_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO9606981.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEILGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVKASWLAKKNQARIDDVVNFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGMSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(10862..11209)
                     /locus_tag="ACHLIF_003003"
     CDS             complement(10862..11209)
                     /locus_tag="ACHLIF_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606982.1"
                     /translation="MKSIYLKYFLSFVFVGVMAMIVCIPFYIVYRAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTFGKANKIASECRKRNEMAEARLVRENELNKIREKQ
                     LQALSDADSAKER"
     gene            complement(11206..11610)
                     /locus_tag="ACHLIF_003004"
     CDS             complement(11206..11610)
                     /locus_tag="ACHLIF_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606983.1"
                     /translation="MRAHYFEGNNIDWFICLCLFLVVICVVNALAMFVFPEKFSLQVS
                     RGKTLVCSALIGCFSFITLVVSASQSFTMDELDVGRYWKNDCKLLEVNIPTGAFTEPV
                     NKLECAGVIVNVPVAQYEEYILQWELYKDKMK"
     gene            complement(11607..11825)
                     /locus_tag="ACHLIF_003005"
     CDS             complement(11607..11825)
                     /locus_tag="ACHLIF_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606984.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMETLNGGGQ"
     gene            complement(11905..12291)
                     /locus_tag="ACHLIF_003006"
     CDS             complement(11905..12291)
                     /locus_tag="ACHLIF_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606985.1"
                     /translation="MKTVLLRYGVTGFLLVYWGLSFANRSLLHEPPLSPDFWKTVRLQ
                     LLLVTASVVVTLVRLYSSRSDVQQELAGIIVLLTMMAHGLALLVPSLWLFIQWRWDGV
                     WLLQWQDFVFGPMLIVLPAAMFWFSD"
     gene            complement(12288..12761)
                     /locus_tag="ACHLIF_003007"
     CDS             complement(12288..12761)
                     /locus_tag="ACHLIF_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9606986.1"
                     /translation="MHKSENEKIDFILDEHERIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRRVLSRQELLWLMSLTEDCPDM
                     HQELLNRLLSGKKLTGLDKREIRSLWWEKMDAMQESATRQHEQDTLRTFIGEDGQ"
     gene            complement(12754..12975)
                     /gene="traR"
                     /locus_tag="ACHLIF_003008"
     CDS             complement(12754..12975)
                     /gene="traR"
                     /locus_tag="ACHLIF_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO9606987.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(13110..13625)
                     /gene="traV"
                     /locus_tag="ACHLIF_003009"
     CDS             complement(13110..13625)
                     /gene="traV"
                     /locus_tag="ACHLIF_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO9606988.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTVHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(13622..13873)
                     /gene="trbG"
                     /locus_tag="ACHLIF_003010"
     CDS             complement(13622..13873)
                     /gene="trbG"
                     /locus_tag="ACHLIF_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFO9606989.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            complement(13866..14186)
                     /locus_tag="ACHLIF_003011"
     CDS             complement(13866..14186)
                     /locus_tag="ACHLIF_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO9606990.1"
                     /translation="MNMRNINVITARSVPDKSVSDDFMHAVLSNCTTRIVLPAPKEFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGHIVVAGQSGGGKSVPVKELIDRIIQKYKE
                     SFDE"
     gene            complement(14173..14760)
                     /gene="traP"
                     /locus_tag="ACHLIF_003012"
     CDS             complement(14173..14760)
                     /gene="traP"
                     /locus_tag="ACHLIF_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO9606991.1"
                     /translation="MANNTSSRQAGHAARYVVARVLRGLFWCLKYAVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEINFVRQTAPDGQFPVRDCPTVVDGKYTEPLNQRCTVIFTD
                     AEGYIENTDSSLESLVLMFWITLALMFMSFAVGTRKYPVRPGKMKCVRVVTADERLKE
                     VYTEDASLPEKIRKYPVYFPDDRTNRNNGDKNEHA"
     gene            complement(14750..16177)
                     /gene="traB"
                     /locus_tag="ACHLIF_003013"
     CDS             complement(14750..16177)
                     /gene="traB"
                     /locus_tag="ACHLIF_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO9606992.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGEVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(16177..16905)
                     /gene="traK"
                     /locus_tag="ACHLIF_003014"
     CDS             complement(16177..16905)
                     /gene="traK"
                     /locus_tag="ACHLIF_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO9606993.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(16892..17458)
                     /gene="traE"
                     /locus_tag="ACHLIF_003015"
     CDS             complement(16892..17458)
                     /gene="traE"
                     /locus_tag="ACHLIF_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO9606994.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNIIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSPETVDASHQALLQYIRP
                     GAQNQMEVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(17480..17791)
                     /gene="traL"
                     /locus_tag="ACHLIF_003016"
     CDS             complement(17480..17791)
                     /gene="traL"
                     /locus_tag="ACHLIF_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO9606995.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSFWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(17806..18171)
                     /gene="traA"
                     /locus_tag="ACHLIF_003017"
     CDS             complement(17806..18171)
                     /gene="traA"
                     /locus_tag="ACHLIF_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO9606996.1"
                     /translation="MDAVLSVQGASAPVKKKSFFSKFTRLNMLRLVRAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMATGNGTVKATFGKDSSAVKWVILAEVLVGAVMYMMTKNVKFL
                     AGFAILSVFIAVGMAVVGL"
     gene            complement(18205..18432)
                     /gene="traY"
                     /locus_tag="ACHLIF_003018"
     CDS             complement(18205..18432)
                     /gene="traY"
                     /locus_tag="ACHLIF_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO9606997.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVTEESESTFKEL"
     gene            complement(18520..19212)
                     /locus_tag="ACHLIF_003019"
     CDS             complement(18520..19212)
                     /locus_tag="ACHLIF_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HFO9606998.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRQNVEIFLSSLEIECQSLGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVILWQINQFPDYPFFCMKKENYYHRDNYVQSVISNMTAKTLVVF
                     CFYTLGYKHINIAKELEITEIASKKRIKKINAEINRKFGSFDLFRNRCITTGVMYEIL
                     SVVSEYIIVNKM"
     gene            complement(19404..19787)
                     /gene="traM"
                     /locus_tag="ACHLIF_003020"
     CDS             complement(19404..19787)
                     /gene="traM"
                     /locus_tag="ACHLIF_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO9606999.1"
                     /translation="MARVILYISNDVYDKVNAIVEKRRQEGARDKDISLSGTASMLLE
                     LGLRVYDAQMERKESAFNQTEFNKLLLECVVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
     gene            20142..20711
                     /locus_tag="ACHLIF_003021"
                     /pseudo
     CDS             20142..20711
                     /locus_tag="ACHLIF_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
     gene            complement(21007..21828)
                     /locus_tag="ACHLIF_003022"
     CDS             complement(21007..21828)
                     /locus_tag="ACHLIF_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9607000.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEVMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRWEDKRNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(21939..22235)
                     /locus_tag="ACHLIF_003023"
     CDS             complement(21939..22235)
                     /locus_tag="ACHLIF_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607001.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVAQSRTDLI"
BASE COUNT         5701 a         5906 c         4948 g         5694 t
ORIGIN      
        1 taatcataat cccgtttagt ttttctgttt tttagttccg catcatttct tttttctttc
       61 attataagtt tcctctgata tttctgataa ttcattcaga ccggtcatgc ggctgtaata
      121 tatttcagac accgaaatcc atcccacaat tgcccccaca aaaaacacaa cagaaacaga
      181 aaaggtaaga cacagcgaaa tgacaatttc tacagggctg gcgggataaa aaagcccctg
      241 acggataaag gggatattca ggctgacgat tattcccgtc gcgatgccgg atattccggc
      301 aatgagtaac accacctgct tatttcgcac cttttttccc tccgtacatc tgcagaacgg
      361 tatccatccg cgccataaaa ccggcggcat ccgttgcccc ctgtaaaagc ggtaatgcct
      421 caagcgtgtt gacgttgacc aggaaggtgg tcggtgtggc caccgggata ttcgggaaga
      481 aggtctgcat cacgtccggt ggcaccggta atgcctccgg aaaggcggta tccccctgac
      541 catccagggt gtagggaaaa acggaaaagc cgtactgctg tgccagctgt ttcagtaccg
      601 ggtcaaactg gtgacagtaa gggcaatgtc cctgcataaa cagcaccacc ttccagtcgg
      661 ccaggttaac ctgcctgccg ttgctgaggc ggaaccagcg gggagccggc ttttctgtcc
      721 tggctgatgt gtctgctgcc ggttgtgcag gctgcgcggc cataccctgc ggattccaga
      781 gtcgctctat ttcatcccgg gtggaggcct gcacggcagc ggctgacagt aacagggtga
      841 acagcagtga tttagtgaga gacatgtctg ctctcctttt ctgcagcaag aatggcggcc
      901 tgacgggctt ccagtgcctc acgctcttcc cggctgacgc gggctatcca tgtatccagt
      961 atctggtatg caccgaacag aacgagaatg gccgcaatca gctctgccag aaaaaaagcc
     1021 atccagggct ttctgtaaac cagacgggca acaatatgaa accagagccc cagggagaac
     1081 acccatacac ctgtaatatc aaggcggggt aaagagaatt tatatttact cataaatata
     1141 tcctgtttat aatatccgcc acactcagca ggatggcaaa taaaaaacca gactgattat
     1201 tattccgata tactcatttc atttttacag cgaatgtaca aaaaacagag gaacacccat
     1261 aaaacaaaca gagcaacatg aaatgctggc atcattaaaa agttatccag aaatctgaga
     1321 agccagatac ctgctgcaat gattagggca agcacagaca agtacaataa cctgctgtta
     1381 cgctcgtaaa gaaaataaag cattaccatt acaacagaca gtgccacata cattaccccg
     1441 gtcacactca gaattaacac attcatcagc cagctataat agcgattcat actgacatcc
     1501 ggatccggaa aactcataaa agccagtaag gttagtaaaa taaccgcaac accagacgtg
     1561 attaaccatc ttttagaaac actactattt tctttatcca tacagaataa aaccatactt
     1621 aacaaacaac atgaattaaa agccattcac catcaggtgt ggggacataa aagagttatc
     1681 tctgtttgtc ttttactctg taaatgagtt catttacata ttaacagggc attatttcat
     1741 tatggtttct gatggctgga gtaaccagta gaatataatc attcctgcac cagaaaacac
     1801 tgaggcaacg gctaaccata gacgcttatg cacaactgac atgcctgaaa aatatcctgt
     1861 atggagtagc agcagcatcc atgacaacac gcctgccaga cccagcaggg caggagggat
     1921 atgccacagt tgatatttca taataacaat accttccgcc atcatcatgg acagggtaca
     1981 aaccacaaaa cagtcccatg aaaaaaaacg cctgtcagca gcaaggaaaa tcactgtcag
     2041 tgacatcact gtcacctggg ccagcaataa aggccataaa gaaataaaat tcatttaaac
     2101 gacctcttta aaaattcggt ttaaagtctt cagaaacgtt caggaactgt tttgccaggt
     2161 cgtcctgcga aataaagccg tatgacaacg ggcgaacact gccctgtttc gggtcaacca
     2221 gcatcatggc cgggaaatat ttcacgccga ggcgctgcgc ctgcccctgg tcagtccggg
     2281 aatccggcaa cagcggatta atcacgccat ccacggaaac gggaataaca ctcagaccat
     2341 acgtatcccg gaagccatta atgacctgcg ccagttgccc gtcgatggga tcctgccccc
     2401 ggtaaaaaaa catgatgccg tagtgttcag ccagtctggc aatcgcctgt cgctgctgcg
     2461 tctggtctgc tgccagctgg ttccgtaccg tgccgttgta atggctgtac tgcaggttat
     2521 agtccagttc gggatgtgcc agcatagctt ttttagcact catggtgaaa agcccggcct
     2581 gttgagtcca gtaattctgc agccggaaat atttcacaaa attatccacg ccgggataca
     2641 gaatggcttc gtacagcgcc cgctttgttg ccgtctgcag cgcggccagt ttttgcataa
     2701 tatccggctc ctgacgggga attacaggtg caggtttatt ttccggctcc ggctgtttca
     2761 tcttttcgtt ataccactgc cagcccgcat ctttccctga cgccggaaaa ataaagcagc
     2821 agagtaacag tggcagtaat gctttattca tcttcattcc tttcatcatt ttttctttca
     2881 tcaggtttct gcaggtcagt cagggcagaa aaacaaaaat acagaataaa caggagcaca
     2941 ccggagtaaa ggataacgaa agcagcagca agcatcccgc ctgccgtgct ttcttttacc
     3001 gtatcccacg accagataat taccggggaa atgacaatcg ctgtaatcaa aagtcggata
     3061 agaaaatcaa taaaacggtt cgacgtgaat atgaccttca tcattttccc cggttatttt
     3121 ccctgcctta ctgtcctgcc tgtttcagct ggtcagtaat ctgttctttc actttctgcg
     3181 tcagaacgcc actgtccggg attttctggt tattcatcag gtcttcgtag aagttagtaa
     3241 agtccagttg gtcgaactta atacgctgca gttcatccac ggttattccc cggcagtcag
     3301 gacttttggc gctgccaaag cctatacgca gttgcccgtt acgcccctgc tgctggacta
     3361 tctgcgccag tttcgaatca aactggcagt agctgcgctt tttctgcagg cagacaccga
     3421 gtaccttttt cgaacagaac tcgccaacac tcaccgtcag tttgttgtcc tttgcctttc
     3481 ccagcgcctt ttcttcactg ttacacttcg ccagccccac atcctgcccc cagccaccat
     3541 ccttacagca gttactgaag ccggctgcgg ccttacgaca gaatttcgcc tcgcccgtga
     3601 aagccctgac atccacacca ttcagggcgg caacatcctt accagctgcg gcaagtgccg
     3661 ccagctgtga tacagcctca ccaaaatcac tgctttttcc gctctgcgcc ttatcgcatt
     3721 caccgtcaag gcagaagaca tcaccaccac agaccatcac tttcccggac gttctggact
     3781 cgcaggagta cgtggcatac tcatgcagac aggttccctc atcggaataa aacgcgcact
     3841 gacgtgaggc cagcgtacag gccggattat cgacatattt ctgacaggtg ccgttatctg
     3901 cggactgtgt gacatattta tcccggtatg cccagcacgc ctgtgtgatg ctccagggtt
     3961 taccttccat cacacctgtt ttcgtaccac ccggctctga acactcagtg cctttcagca
     4021 cgccatccgc tttattaaac ggacaactct ccacccactc tacccgtgga acccattctt
     4081 tatcacggac cttcatcctg agtttcagcg taaaggtgga cgcgccgctg gccagtttct
     4141 cataaacctg tcggtcaccg ttcccgccag aaagacagtt accgttagca gtacagccac
     4201 tgccggtcag tacctgcccc tgtgtcacct gcaaacctgt cgccccggac aaggggaaag
     4261 tccccgactg cccgtttacc aggccaacat tgaacgttga attcatgaag gtataacgag
     4321 aattcaggaa gaaaaaagcg gcgtatacag acaatgatgc actttccact gcaccagtta
     4381 ccggagccgt gacactgaac accagtttca gcccgttcat actgaaccgg aactgtgagt
     4441 gcggtatcgt cacctcacga tattcatccg tgtatttcca gtctccggtg atcctggcag
     4501 tccgtgtgca gaattcctcc accatcgtgt cccgttcaca ggtatagttt gtgaattcac
     4561 tgcggtttat ctgctgcgca ctgcactgct gcccggtgtt tcccacaatg ctgtcagccc
     4621 ggttcaccac atccctgccg gtctgaataa acggtgcatc cggtgacaga atgtctttcg
     4681 gcttgttcat gaaggactct gtgatggttt tgcctgtttc accggtcgcc cattccgtgg
     4741 tgccgttatt cttcagccca ccatccccgc cggcagtcac gccaccgtaa tactttgtct
     4801 catccgggct ggcgttataa ccaggaatgc tctcctgcgg cttaaagttc tgaatactgc
     4861 cggtaccctg cccctgaatc tgacgggcaa aatcagaacc ggcccggtaa tcactgttcc
     4921 tgtcagcctg tgccatgcag gcaaccaggg gcagtatcag aagtaaaata cgtttcattt
     4981 cccggaatct cctttgcctg ccagtaaatc atgagccacc tgccggcagt ctcccgcgct
     5041 ggccactttt tccagagcct gcctgacgcg cagatttccc cggatgatgt cgtgcccctg
     5101 gctgcagaaa accaccagag ccggcacgct gcggatgcca tactgtgaaa acagcgtcgg
     5161 gtcgatctgg acaccatcag tcgcaccgtc cttcaccagt gccagcaccg cttcagccgt
     5221 ggccttcagg tcattgttca ccatgccgcg cagcgtggcc ggaataccgt agtgccgggt
     5281 ttcacccagc atccgtttca gtccctcttc gggaatggag aaggacacaa aatacacggc
     5341 accctgctgt ggtttatcct tctgagcagc ctgctgttta cggaccagtt catcgagaaa
     5401 ctgattatcg gaatgctgaa ggggattctc cctgacctgc tgctctgccc atgcctgcaa
     5461 ctgtgtgtcc ggtttttcac gtaactgctg actcagtgtt tcctgctgtt tcaggaactg
     5521 gcggttttcg ggcgtattca tattttccga agcatgggca gaaatataca gccctgctgt
     5581 cagggcaaaa agcaccacta ttttccgtcg cattgtatat cctgataaca tcgttactgt
     5641 ttaattttac gggataaaca atcccggata ttcactctgc taaagcatta attcatttct
     5701 ggccgacaca cataaaagga gccagatatt actcacaaca ttatctgtta ttcacttctt
     5761 cattttggtt aactttcttt cgcggcagaa actcatcaga gcaaagaaat tcaaaaaaaa
     5821 cagccgtcaa agcgcacaaa aaagttattt ccagacataa tctgataatt ctgagtctgt
     5881 ctgatacatc acttttctgc cattcgctga taatgtcact gataacattt tcaggtataa
     5941 aataaaacaa cactgtgaat accatcacca gtgcaaaagg aaacagtttt aatgttttgc
     6001 tgatcaaacg aatgagcacc aaggatatac tcatataaat atgaatgtat ttactgctta
     6061 atttcttttc catttaaaat attcctcttt ttgtttaatt tacagaaaca cacagttacg
     6121 tttacgccac atcaggtaac cgaagttttt ctttgtgttg ggcggatttt ttcccgtctc
     6181 ccagcgcatc acactgcgcc cgaacggatg gcactgcccg ctgtccgggt acatgttcac
     6241 catctggtaa cgccagcgtt ctttgggaag aatgggcgac ggatactcat aacagaccgc
     6301 cgtgttctgc ccgatggtgt tcataatcat tccctgacgg tgcagcttga atgccatccg
     6361 ttcactgacc agcagggagg actgcagcgg gctggattca ttgctcaccc agccgttgaa
     6421 cgggtacatg ctgccctgcg aacctgcaca ccagaacaga atatccagtg gtttattaaa
     6481 ggcgctggca atcgcgtccg ctgcacaggc cccctgggct atcggattgg caaaaatgac
     6541 cgcctccggg ttgagaatgg tggtcaggct gctgtccgtc caggtggggt cgatttccga
     6601 gatgtacccg atatccatat ccccgcccat cagacaccct gcagacgtga taatgttcag
     6661 ccagtacgtc accgggtatt tgtaccagtg aacgtgatag aacgcgccat tcacctgcct
     6721 gtcatctttt ttggccgttc cctgtccggt tttccccagc gccgggatcg tcattcccag
     6781 attgaccata catcccggcg accgggtgac gtccgtcagt gccatcggct cccagtaccc
     6841 gatggccagt ccgatacgtt taaaaatggg gggaggtgcc gggcagaact ggatcggcat
     6901 cgacgggttt gcggtatccg gcacctttcc ctggctgact tttgtgctgc ccagcgacag
     6961 cgggaaaatg caggtccagc agatatcggt gatcgggttg acgaaacgcc cctcacaggc
     7021 cgcatccgct gacaccacag ggatatgacc ggccagcaga aaaaccacac acagccacag
     7081 gcaccgtttc attttctgtc ctcctctgcc ggtataaact ccactttcag gaagcgatcg
     7141 ccgggaacgg cgctcacccg tgccgggacc tgatcgatgc cgaggcgctg gcagagcaca
     7201 ccgttctggt caaagtaaat ccggctgtcc agagacttct gcatctccgg gatactgccc
     7261 tgcacgagga taattttgct ctccagtgtg ggcggcgtct ggcgtttcat ccaggctacc
     7321 tgcgccggat catcgccatt gatgaaatac agcgtctggt taaaaggaac atactgcagc
     7381 ggattcatca cttcaccctg acgggcaaac acccgccctt cgttatcccg gatatccgca
     7441 gccagtctga cggacgggtc aaacaaccgg ctgccgtatt tctctgtacg tccgataccg
     7501 ggcacggcag ggggccgcag gctgttacgg atcacccgct ccttaaaggc atccattttc
     7561 cggcccatct caccggactg ctccagtgcc gtcaggcgct gcatgatcac cgtcagcatg
     7621 tccggctcct ttaccggcca cagatcgccc caggtgccaa gatcggcggc ggccacactc
     7681 tgcccccata ccagcagggc aatcagcccc cggcatctca tggttccgcc ctcattcgcc
     7741 gggcaatatc ctgctggatt tcccgggtga tatccggtgc cccctgtacc acagcaggcg
     7801 acaccagaat gaccgcgtgg tgtttctgct gccacgcctg caggctggct tccagtgccg
     7861 tgttaaaacg cgctgaaagg gcttttgact gtacttctga tagttgtttc tgactggcgc
     7921 tgtcaaaaaa cgcatccacg gtctgtttca tgttgaaggc gacagtcacc ggtgcgttca
     7981 gtctgacaat gccgtaactg acagctgcgt tcagtaacac cattgccaga catcctggca
     8041 ccgtccacca ccagtggctg cgtcgttctg ccgtgacgtc agtcgttttt tgcgttgtgg
     8101 tcatgcaaca ctcctgtatt tctcatgttc ttccagccag gcctccagcg aagccatctc
     8161 cggccccgat ttcttccacg ccagctgcca caccgcctca tgaatactca taccctctct
     8221 gcgtttctgc tgcacaaact caaaatccgg gccgtcagag ctgtacatgg cacggcttaa
     8281 cgggtccaca aacagacggt gccaggagga atggttttcc acctgcagca ggaaggaact
     8341 gaaccactgg tctttggccg caccaaactt accaatcatg tcgcgctgca gtggctggaa
     8401 ctgatccggg aataactggt tatatttcgc gaactccttc gcactctgtt tgaggataat
     8461 tttgtaggag gagttacccc atgccgcacg ggctgcgctg gatgccttgt cagagtcaaa
     8521 gtcgacgatg ttctgtgtga tggtgatata ggcaccggta tgacggcgac aggtgcggta
     8581 acctttttga ataaactcac caactttacg atttttgaag tccagcagac gccagccttc
     8641 atcaatgacg ttcagtttct tgagggtacg cggcgtgcgg tacatccggt tctcgatgta
     8701 aatgataagg ctgaacatca ccgcaaccag cagtgacgga cggtcttcca gcccacccag
     8761 ctccagcacc accattctgg catcatcccg cagggacggt tcatcagagt taaaataccg
     8821 gccgtaagtg ccgttggcag tgtactggtc aagcagcaca atcatttcat ccagacggct
     8881 gcggatggtc ggtgacccgg catactgctc gctgtcactg gcgtttttca ggaaattcac
     8941 cacatcatca atgcgcgcct gattcttttt ggccagccag gaggctttga ccgcctgcag
     9001 cagcagacct tcatgcactt catccaggtt accgttgggg ctggccatca ccgacaactg
     9061 gtcgcggaca cgctccgctg actggtcgat atcggtgatg ttcgcaaacg ggttaaaacg
     9121 cagggtttca ccgtccagat atacgccccc catgttctca cacagggact tgtagccatc
     9181 ccccatgtcg aacaccacgg caaagccccc ggagtccagc acgctgcgga taagtggctg
     9241 tatcagtccg gttttaccgg ccccggaggt gccacagacc gccatgttgt agttggtgtt
     9301 attcatcccc ttaaagaaga tatcgataaa cgccagctgg ttacggtagg tgggcgccag
     9361 cagacctgcc ggtgtcaggg gattatccgc cactaacggc atcaggttgg ccacattaaa
     9421 gctctctgcg cgctgaacca caccggcctc tttcagctgt ttaaacagcc ctttcccggc
     9481 cataaagggc aggcaggtca ggaaattgcg catgtggtta aagcgcggtg aaatcagctc
     9541 aaaaccgttt ttacggaagc tgttcaggat gtcctgctcc acttccagtg ccgtttcatt
     9601 attgtccttg cagaaggctg tgatgttgag gaagtaggac acgacagaag actggccgga
     9661 gcccagccgc tggcgcagtt ccccccactc cttcgcctcc ttttcaactg atggaaacca
     9721 tttggcatag gaggtcttcg acttcttctc caggtccatg tacttcaggt tggcttcgct
     9781 gtgggttttc acctggtctt ccaccaccag agtcagcgtc aggatgaacg ggcaggaaat
     9841 ggacatttcc gggttcagca gattgctgta gttgtcggcc atgttccaca ggaaggcgat
     9901 ttccgggtta cgggccagat ggaaattcag gatgcgggcc gtgctgttcc tgccgttctc
     9961 acgcaggccc agcgtcaggt aatcagcccg gacattcagg tcaaaactgt cctccacaca
    10021 ctgataattc agatcagaat acgggtccag ctgacgtctt ttcgggtaca gagagtccgg
    10081 attatggtta atcatctccc cgacaatctc gatgaaagcc tgtgcatcca ctgtctgtgt
    10141 ggtgattttt gcgccgtgaa aagaggcacg gatgattttc accagatttt ccatttccag
    10201 aatgtcagcc cggctttttt tcttcgaggg agaacagtac gagataaaca cccggtaatg
    10261 gcgcagggtc aggggcagat tcatcccctc cggcagcggg aactgtgtcg ctgccgcttt
    10321 catataatag gcacgggtaa tggcgttaaa ccgttcagcc tgttcacctg accaggagaa
    10381 ctcacgcagc ccgtattcaa tcctgtcacc aaccagctga ctggacatca gatggataca
    10441 caacggaata ccgcgcggca gtttggtgcg cagcatatga tcgagagcct ccacaatgga
    10501 cttattcgcc ccattgatgg gaatggcttc cagcataaag cccatcgtgg tgtcattcat
    10561 gaacagaccg gattcctggt tgtaatcacg gtacggcagc agacggctga actgcgggaa
    10621 actcatttca cccagtattt cattggcctt tgcggactcg tcaggcagct tcagtgctgt
    10681 gacgagagag ttaaccgcct gagtgacggc ctcaagtggg ttattactca tatgagtatt
    10741 ccttagtacg tttaaaaaag gaaaatatca atctgtggca tggatatgtc acgcagtgcc
    10801 atttctgaaa ccacagcaca aataagagat ggaggctttt aacaggaaac caggattaca
    10861 attaacgttc ctttgctgaa tcagcatcac ttaatgcctg aagttgtttt tcgcgtatct
    10921 tattgagctc gttttctctt accagtcgcg cctctgccat ttcgtttctt tttctgcatt
    10981 ctgatgcgat tttatttgcc ttaccaaagg ttaaaggttc agactgataa ttaagtgtct
    11041 cctgaaatgc ttctgctgca cagggagttt cctgtaaaat ctctgtcagt tgttctggtg
    11101 ttgcaggttg ttgcgcacgg taaacgatgt aaaaaggaat acatacaatc atagccataa
    11161 caccaacaaa tacaaaagac agaaaatatt ttaaatatat cgacttcatt tcattttatc
    11221 cttatataat tcccactgta agatatactc ctcatactga gctaccggca cattaacgat
    11281 gactccggca cactccagtt tattaacagg ttcagtaaat gcgcctgtcg gtatattcac
    11341 ttccagaagt ttacagtcat ttttccagta tctcccgacg tccagttcat ccatagtgaa
    11401 tgactgactg gcggacacaa caagcgtaat aaaagaaaaa catccaataa gcgcactaca
    11461 aactaatgtt tttcctctgc tgacctgcag actgaatttt tcagggaaaa caaacatagc
    11521 cagtgcgtta acaacacaga taaccacaag aaagaggcac aggcatataa accagtcaat
    11581 attatttcct tcgaagtagt gagctctcat tgcccaccac cattcagtgt ttccatcaca
    11641 ttgttcaaag gttcatttgt tttaacttca atacattctc ctgtatttcc aaagcccctg
    11701 cctccgtgcg cttcgattgt ccgaagaacc tgcacgcata cgtccgtatt tggcatgggg
    11761 gtaacattcc agcttccgcc accgttaata ttttttatca gcacaagaaa aataaaggta
    11821 tgcattcttt attaccaccg ttcatatatt aattactgaa tataccttcg gttaaccctg
    11881 agatatcggg tacacctttg aagattaatc agagaaccag aacatggcag caggcaggac
    11941 aatcagcatg ggaccgaaca caaagtcctg ccactgaagc agccataccc catcccaccg
    12001 ccactgaata aagagccaga gggaaggaac cagcagagcc agtccgtgcg ccatcatggt
    12061 taacaggacg atgatgcctg ccagttcctg ctgaacatcg gaacgggagg agtacaaccg
    12121 gaccagcgtc accacaacag acgcggtgac cagcagaagc tgcagccgga ctgttttcca
    12181 gaaatcagga gaaagcggcg gctcatgcag taaagagcgg ttcgcaaagc tgaggcccca
    12241 gtacaccaga aggaacccgg tcacaccgta gcgcagaaga acggttttca ctggccatcc
    12301 tccccgataa atgtccggag ggtatcctgt tcatgctgac gagtggcaga ctcctgcatt
    12361 gcatccattt tttcccacca gaggctcctg atttccctct tgtccagacc ggtcagtttt
    12421 ttaccggaca gcaggcggtt aagcagttcc tggtgcatat ccggacagtc ttcagtcaga
    12481 ctcataagcc agagcaattc ctgacgactg agtaccctgc gtgattcctc cggtacgctg
    12541 taataattaa aaatacgact gaatacccca caacagcagg caacggacag gcacagggat
    12601 ttcagataca ggccagaaac acagaatgtg gcaacaccac aaagaatagc tataactaaa
    12661 aagcccttac ccagtgccat acacaaagaa ccctgttttc tgaatatccg ctcatgctca
    12721 tccagaataa aatctatttt ttcattttct gatttatgca tagtgtttcc tctgcctttc
    12781 ctgatacgcc tgacattcaa cacataacgt cacaccggga aatattttcc gccgggcttc
    12841 cggaatagga tttccgcatg cttcacagag ataaacagga ataccatgag catttatttt
    12901 ctggcgtatc cggtttattc ctgtcatggt cagttgttct gtcactgaat atgcttcatc
    12961 ggcttcatca ctcacggaat attcctcccg acatgttctg acacgctacc gcccatccct
    13021 tttcacgcac agggcactcg ttttacaggc agattttgaa tacagaaagc ccgccggcat
    13081 tgccggctga tatacaactt cagggccagt taattaatac gtggttttcc ccacgcagac
    13141 ggtttaataa caaaaaatac gctggatggc tgatgataaa catcctggtt atcaatataa
    13201 ggcgctatcc ataatgctgc cgtctgctca cccgttctta gcggacgagg aggtgttacc
    13261 ggtgcaactg aactgaccgg aacaaccgtt ttcacttccc gggcagcggt aaacagcggg
    13321 cgtggtgcca gcagtttctg ctccggatgt actgtcaccg caggtctgct gccggacgga
    13381 gtggtcgcgg tgacggtctg caccggcatt gtccggaagt tcccttcagc caggcgcggc
    13441 agtgatgccg caaccggctt tgcttctgag gactgctcca gttttttggc cttctcattg
    13501 gcctgctcca tcgtcataca ggtatcggat gtggtggcgt tacactcaaa ttccgtactg
    13561 gttccggcac agccggaaag taataaggtc cccagcagag gaataaataa agaaatctgt
    13621 ttcattgtcc ctcacctttc tcatcgcagt tcacaaaatc attcttcaaa tccctgaccg
    13681 tacaggtttt tacaatgtca ggattttcaa aaactgtatt ccgtgatgat ttataaacat
    13741 atgccacaat gcgttttccc cccgcatcag gttctggtgc actgacttca cataacggag
    13801 gtgttattcc tgactcatac agcaccggat aatttatttt tttgacgctg ccatcagaaa
    13861 ccagcttatt catcgaagga ttccttatat ttctgaataa ttctgtcgat tagttctttc
    13921 accggaacag attttcctcc accggattgt ccggcaacca caatatgccc cggagaagaa
    13981 aataagctca tcgacatacc cttcttcatc acaccgacag cagccatatt aaaattgtgc
    14041 ggcaatgatt cagaactgaa ttccttcggt gcaggaagta cgattcttgt ggtgcagtta
    14101 ctgagaacag catgcataaa atcatctgat acgcttttat ccggtacaga gcgggcagta
    14161 ataacattaa tattacgcat gttcattttt atctccgttg tttctgtttg ttctgtcatc
    14221 aggaaaataa acagggtatt tcctgatttt ttccggcagg gaagcatctt ccgtataaac
    14281 ttccttcaga cgttcatctg cggttaccac acggacacat ttcatttttc cgggtcgcac
    14341 cggatatttc ctggtgccca ctgcaaacga cataaacatc agtgccagcg taatccagaa
    14401 catcaaaacc agactttcca gcgagctgtc tgtattttcg atatacccct cagcatccgt
    14461 gaaaatcacg gtacaacgtt gattcagtgg ttcagtgtat ttaccgtcga ccactgtcgg
    14521 gcagtcacgg accgggaatt gcccgtcagg ggctgtttgt cttacaaagt taatctcctg
    14581 aaccagtaat tttcccggcg tggtgttgtc cttccataaa acaaacagag ccatcagggc
    14641 cattgtggcc agtggcagaa tgacggcgta tttcagacac cagaacagtc ctcgcagaac
    14701 ccgggcgaca acataccggg cggcatggcc cgcctgacgg gacgacgtat tatttgccat
    14761 cattgccccc tccctgactg ccggacataa actgctgcgg attcaggttg cccagttgct
    14821 tcagcatctg gtcagtatta aagccgttaa gatgactttc agctgatggc ggaacaggaa
    14881 ccggactttc cggattgtct tcttccgctc tgctctgcgt gcgttccagc gccatctctt
    14941 ccacggtttt cagctggaag ccgtcctgga acaccacggt cacttcgttg cccgcaccaa
    15001 tcggtatcac cgggtgatac tgttcggcac gtttgatgta gtagtcactg agcgtctgtg
    15061 cggctttcga tgcgccgcca ccgataccca ttttaaggac ctcgccggcc ccgtaagcag
    15121 cagtggcacc cagcccgaca gccggctggg aagcacgctc cattccctga ccgataccgt
    15181 caacaaatcc cgcgccccat gcccagccga ggattttgcc gttacgcatc accacttcgc
    15241 ccttgatacc gtttttaccc cggaagctga catgcccctt aatcggcata tcaatagttt
    15301 tgccgtcctt caggcagctg atattgcggg tgcgtacaat ggcacgttca ctggacacat
    15361 ccccccaggc ttccagaccc acaaaacatc ccgttgcgtc atacgtcttg ctgttcggca
    15421 tttccaccag gccggtgata cgcagctgca tcggcaccgt ggattcatta ccggtgacag
    15481 aggcattggc atccgcccct tcaatcagca tggctttcgc aaaacttcct gacggaatgt
    15541 acggcaacga tggtccctgt tttccctcat tacgggtgaa caccttacgc tgtatccggt
    15601 taggcaccgg tacagactgg tacgtcacct gcggtggtgg cgtgacacca ttcccgggat
    15661 aaaacgccgt cggcggtgga acagcaacgc tgccctgcgg cgggaaagat acgggtgtgt
    15721 ttcctggctg tggttcgcct tccgggccgg gcggtgaggc aggcatctgc ggtacaggct
    15781 caccggtcgc cgtgacggga ttagcgccca gggcttttac ctgctctgcc agggcggcat
    15841 tgtcctgtcc cagcttttca atacgacgct ggtcatcacc gcgctgtttg ttcagaacat
    15901 ccagctcacg acgaatttcc tcatactgct tctgcatctg cgctgccgtc acctgcatct
    15961 ctgtggtggc atgctgacgc accttgtcat caaaggtcgt atccaccaca ccggtcatat
    16021 ccggcacagg ctcctgttca gccacggttt caccgttacc ggacatgtcc acatcagaca
    16081 gatacagtgc cccaccaatc gcggaggctg caccgacaac cacaatcccc agccacaggt
    16141 actgcttgcg tttaacaatg gtattgatac tggccatcag ttgccctccc cgtcacggat
    16201 gacatacaca tccatgcgcg cacctgccag taactggctg gcaggctggc tgaacatcac
    16261 ggcacgggtt cccggctgcc agaagtcact ttcccggata ttcagggcag acagcgtttt
    16321 attttccaca gcaaagcgga ccattttcag gtgattcccc gtccatacgg catctgccac
    16381 tgaagacagc cccgccgggg cctgcagggt ttcttttgtc accggaacct gataccagcc
    16441 tgcaggtaat tttccgccac ggacggcctg gctgatggtc acaagcaggg attcgtaagg
    16501 cgtggacgtt tcccacgcac cggcttcttc tccggttccg cgcaggtcac tgaccagttg
    16561 aatggtacgt cccgcgcctt cacggggaac ggcctgaatg gaaagattca gaccacgttc
    16621 tgtttccaga atgaacgtaa agggcttttt gttgacggtg gccaccacca caccgccgga
    16681 ggcggtctgc tcattattgg tcagggcacc atccagactg ttcacggcga taatccggtc
    16741 accgggaacg gtaaacagat tcgggctggt attgctgatg gcaatactgg cctgaccacc
    16801 gttcaccatt ggcaccacgg tgggggccag cgtaccgttt gcggccatca cgctgcagga
    16861 aaaaaacagg ctgccgaata ttattgccgt attatttttt ctcatcgtct gtttccccga
    16921 aattatccag ccaggtcacc ccgttttccc gcttcagaat aaggatgtaa tgtttgatat
    16981 cagtgaaagg ttttgaatca ccaatccagg ttttaagcac accacgaatt tccacacgac
    17041 catactgagg ccagacacga acactggtct ggaaaaaggc tgagttcacg ttatcgtttt
    17101 taatacgctt cgcttcttca gccagaataa cttccatctg gttctgtgcg cccggacgga
    17161 tatattgcag aagcgcctgg tgtgaggcat cgacggtttc cggtgagaca ttcagacgga
    17221 gagcaataaa tgacagtgcc atctgctgta aataagaggc atcggcactg ttctgtgaca
    17281 cggcaaaagg agcattaaac gccattggcg tcacggcagt gcgttgttca ttttgaagac
    17341 ggtagttatt caccccctga atgatgttaa cagagaggct gagaacaata agcactgaca
    17401 taaatataaa ggcgatggcc attacacgac tggtacttaa acgagcaccg tgttccatat
    17461 aacaatcctg gtatcagttc tatttaatcc actgccggaa acacgaatcg ggaacattat
    17521 gaaaaatacc gcgcagcagg gccgttggca tataccagta aatcaggtca cgtaaccaga
    17581 aactgccccg cccttttttc agttttttaa tcccgaaata aaccagaacc gctgcaccaa
    17641 taccgaacag atatttcgat gttgtgatac cccagccaat acagattgct gcggggatca
    17701 gttcatccag cggaaggcca aaccagcggc tctggttggt cagtgtttcc gggaaacgat
    17761 atttcttaag tttattctcg tctcccgaca tcgttttatt tcctgtcaga ggccaacaac
    17821 ggccataccc acagcaataa atacagagag aatggcaaaa ccggccagga acttgacgtt
    17881 tttagtcatc atatacatga ccgcaccgac cagaacctca gccagaataa cccatttcac
    17941 cgcactggag tctttaccga acgtggcttt gaccgtaccg ttaccggtgg ccatcaggtc
    18001 ctgaccacta ctgccagcgg ccatcgccag ttgcgggaag aacatcatca gaacagcagc
    18061 cgggatcaca gcccgaacca agcgaagcat attcagacga gtgaatttag aaaaaaacga
    18121 cttctttttg acaggcgcag aagcaccctg aacacttaaa acggcgtcca tatgattcct
    18181 ctgtttaata ttgatagata aaaactaaag ttctttaaat gttgactcgc tctcttcggt
    18241 cacctccctg aatatttctt cgttatagaa atcaggaaac cgtttcagat gatctctgag
    18301 acggatctgg acttctatcg ttttacttct tccactacga tctttggcct taataagtcg
    18361 gttatttgta tcttcatcga gatacaccga gaccgttctg cttataccgc ctctcgcgtt
    18421 acgcctcctc actaaattca cctctgtttc ccgaccaata aaataatgat tgcacagaaa
    18481 tagcctgtcg aaaatgggcc caaaacgagc cctgctatgt tacattttat taacaataat
    18541 atattcactt acaacactta atatttcgta cattacacct gttgtgatac atctattcct
    18601 gaataaatca aaagaaccaa actttctatt tatttcagca ttaatttttt tgatcctctt
    18661 tttagaggct atttctgtaa tttctagttc cttagctata tttatatgtt tataccctaa
    18721 tgtatagaaa caaaaaacaa ccaatgtctt tgctgtcata ttagatatga cagactgtac
    18781 atagttgtct cgatgataat agttttcttt tttcatacaa aagaatggat aatcagggaa
    18841 ctgatttatt tgccataaaa taacagattc atcattataa aatgaaacat tctccatcct
    18901 tatttgatag atttcgccat gaaaattaaa acgtcgacaa aatgcagaac catgtccaag
    18961 agactggcat tcaatttcaa gtgatgaaag aaaaatttca acattttgac gtatataact
    19021 ctctccggat aatggtttat cttctctgga aaagagttca ataaaagccc cattggcata
    19081 aaggattttc cggtttttgt tacggacaca aaccggaacg ggagaatttc tgtagatatt
    19141 ctgaacgtta aggatgtatt tatttacaga ttgactgtta agtggccttt ctctacgatc
    19201 cagcgcacac ataggaacct ccgttaagga ttctatggac agtcgataca gggagatctg
    19261 acctccctgt atcggcgatt tttcatttaa attttaacgt ggcattaacc acataattaa
    19321 tatactcgtt atgtggttaa ttgtcatcaa gttgaaccag atcaatattc tgttatttga
    19381 agcaaatcaa cttttaattc tttttattcc tcatcatttt ttggaaagaa tctctccatt
    19441 tccgatgaca ctttttccct gatatcttcg accatattcg catattcaaa ctttggattc
    19501 ccggaaacat gaggactgag tgactcaata cctaaaatct ttgccaccgt tgactgtgtt
    19561 tttacaacac attcaagaag aagtttatta aactctgtct gattaaacgc agactcttta
    19621 cgctccatct gagcgtcgta tacacgaagc cccaattcaa gaagcattga agcagttcct
    19681 gaaaggctta tatctttatc tcttgctccc tcctgacgac gcttttcaac tatcgcattt
    19741 actttatcat agacatcatt actgatatac aaaattactc tggccataag acagtacctt
    19801 ttagattctg aaataagtgt tttatttcat ctactcatag tatggtctga aaaaagaaga
    19861 gcacccctag cagcgcccct agggtcatac tataaaaaaa cgcacggcgc tgctaggggc
    19921 ggccctaata tatccaaatg tttttcatga aaattgtcag tactgaccct aacaagggtc
    19981 gtcatagggt cgccataggg tcgtcaacga ccctatttaa taatgtaaaa taaattaaaa
    20041 tacattattc ataacataag ctaatgattc aaaaaacaaa tcagcaaaaa cttgtttttg
    20101 cgtggggtgt ggtgcttttg gtggtgagaa ccaccaacta gttgagcctt attgtggagt
    20161 aggttaaatt atttcctgat gacgtcacca gaggtgagaa aatgagaaaa tggcttctga
    20221 cgatctgcct gttgtttata aatgtgacct gcgaggctgg tgattgcttt gatctggctg
    20281 gcagagatta taaaattgac cctgacttgc tgagagcaat atcatggaaa gaatcccgtt
    20341 accgggttaa tgccatcggt attaatccgg taacgggata tggcagcgga ttgatgcagg
    20401 tagactccca gcattttaac gaactggccc gctacggaat taaaccggaa catcttacag
    20461 cagatccctg tctgaacatt tataccggtg cttattatct ggctatagcc tttaaaaaat
    20521 ggggagtctc ctgggaggct gttggtgcat acaacgcagg attcaggaaa accgaacgcc
    20581 agaaccagcg gcgtcttgcc tatgcatcag atgtttaccg gatttacagc gggataaaaa
    20641 acagtaaggg gatccagctt cgtgcatctg aaaaaccgct ttcaaaaatg aacagctcgc
    20701 agaagaatta atttgatata cattttaaaa aaccggttat gccgcaaatg cggcatggaa
    20761 ggccacacac aaaaatatca tatgagtgat aatgctgact ggccgaagcc ccgcaaagcg
    20821 ggaacccatc ttatatatat ccggtaactg cgcagcctcc tgccagacca tttttcgcgg
    20881 gcacaatatt tacagactac cgaaaagcca gttgatgaaa ttgaggcatc tcagacgaca
    20941 tatcaatgcc gcataatgat aatataagta aagccccgaa aatttttcgg ggctttactt
    21001 atattatcat gacttccact ccctgaactg ttcggcaatc atccataatg ctttgttgat
    21061 tcggatatca ccatcaatac ccgtaatggc tcttgtcctg gtatttttac cggaggcact
    21121 tcgccccgac agtccacctt ttatcatatt ctcctgaacc cgctgccagg ttgtccagag
    21181 atcgttccgt ttatcctccc aacgacgggg agtaattatt tgttcaggcg tcactggtgt
    21241 tttattttca tcttcatacc tgaccatcag tgcagctctg ccaaataaat gttgctcgtc
    21301 actgttaaga tgtatttctt tcatcacctc catattatca gtgaccttat caaagacacc
    21361 gagcacttca tacgcaccct cgataacctg cccgacaata tcacctttat gtggaacgcg
    21421 gatttcgcca aaattattcc cgcacaccag gccatttgtg cagacaaaac gaaaaattcc
    21481 tgggatcatc tgataactgg atgacccatc atgtgaatta agcaggataa tttcaggaac
    21541 ttctttcccg ttaatatgcc cttccctgcg aagacgtaac atatgtttac tgtattcgcg
    21601 gcgtcccaaa tcacgaaccc gactctgaca ggcaaagaac ggctggaaac cttcatcacg
    21661 taacttattg atgatattga ttgttgggat atatgtataa cgctcactcc gggattcatg
    21721 tttatcacca gaaaagacag atggaacaaa ctgcattaat tcgtcatccg ttaacgggcg
    21781 ctcacggcga atagaattat atcgaccaaa acgggaagct aatcgcataa cagtaactcc
    21841 tttatgaagg cataaaatta ataaaaattt tttcgcttaa tatcatcccc cctcggttat
    21901 cgttaacaaa aagacaacaa ttccgagggg aaggaccatc aaatcaggtc agttcgggac
    21961 tgagcaacct gccgggtgaa aagcagtaac agcgcgatca actcatcagc gttttttctg
    22021 acaagaggag tatgattctc cagactatcc agtaagtcat gcagctcatt attgacctgg
    22081 tggagcacct taaagagtgc accagaatcc cggcagctct gatatgcaag atttgcctga
    22141 acagcacgat tctgcatgat cccgatctgt gcgattgcgt ctttctgctg atcgccggtt
    22201 ggctgataag tggtgttaaa agaaaatgcg ctcataattc ctcctgtat
//
LOCUS       DAZGOB010000073        22096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGOB010000073 DAZGOB010000000
VERSION     DAZGOB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.073"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            139..867
                     /locus_tag="ACHLIF_003024"
     CDS             139..867
                     /locus_tag="ACHLIF_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO9607002.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKKINAVSTLITRNSEHH"
     gene            867..5717
                     /gene="ehaG"
                     /locus_tag="ACHLIF_003025"
     CDS             867..5717
                     /gene="ehaG"
                     /locus_tag="ACHLIF_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO9607003.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKTTNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITASTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6086..7741
                     /gene="lldP"
                     /locus_tag="ACHLIF_003026"
     CDS             6086..7741
                     /gene="lldP"
                     /locus_tag="ACHLIF_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO9607004.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7741..8517
                     /gene="lldR"
                     /locus_tag="ACHLIF_003027"
     CDS             7741..8517
                     /gene="lldR"
                     /locus_tag="ACHLIF_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO9607005.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8514..9704
                     /gene="lldD"
                     /locus_tag="ACHLIF_003028"
     CDS             8514..9704
                     /gene="lldD"
                     /locus_tag="ACHLIF_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO9607006.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9890..10363
                     /gene="trmL"
                     /locus_tag="ACHLIF_003029"
     CDS             9890..10363
                     /gene="trmL"
                     /locus_tag="ACHLIF_003029"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO9607007.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10416..11237)
                     /gene="cysE"
                     /locus_tag="ACHLIF_003030"
     CDS             complement(10416..11237)
                     /gene="cysE"
                     /locus_tag="ACHLIF_003030"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO9607008.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11317..12336)
                     /gene="gpsA"
                     /locus_tag="ACHLIF_003031"
     CDS             complement(11317..12336)
                     /gene="gpsA"
                     /locus_tag="ACHLIF_003031"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO9607009.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12336..12803)
                     /gene="secB"
                     /locus_tag="ACHLIF_003032"
     CDS             complement(12336..12803)
                     /gene="secB"
                     /locus_tag="ACHLIF_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO9607010.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12866..13117)
                     /gene="grxC"
                     /locus_tag="ACHLIF_003033"
     CDS             complement(12866..13117)
                     /gene="grxC"
                     /locus_tag="ACHLIF_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO9607011.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13259..13690)
                     /locus_tag="ACHLIF_003034"
     CDS             complement(13259..13690)
                     /locus_tag="ACHLIF_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO9607012.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13935..15479
                     /gene="gpmM"
                     /locus_tag="ACHLIF_003035"
                     /gene_synonym="pgmI"
     CDS             13935..15479
                     /gene="gpmM"
                     /locus_tag="ACHLIF_003035"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO9607013.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15513..16772
                     /gene="envC"
                     /locus_tag="ACHLIF_003036"
     CDS             15513..16772
                     /gene="envC"
                     /locus_tag="ACHLIF_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO9607014.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16776..17735
                     /locus_tag="ACHLIF_003037"
     CDS             16776..17735
                     /locus_tag="ACHLIF_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO9607015.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMRALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17722..18756)
                     /gene="waaH"
                     /locus_tag="ACHLIF_003038"
     CDS             complement(17722..18756)
                     /gene="waaH"
                     /locus_tag="ACHLIF_003038"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO9607016.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19007..20032)
                     /gene="tdh"
                     /locus_tag="ACHLIF_003039"
     CDS             complement(19007..20032)
                     /gene="tdh"
                     /locus_tag="ACHLIF_003039"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO9607017.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20042..21238)
                     /gene="kbl"
                     /locus_tag="ACHLIF_003040"
     CDS             complement(20042..21238)
                     /gene="kbl"
                     /locus_tag="ACHLIF_003040"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO9607018.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21513..>22096)
                     /gene="yibB"
                     /locus_tag="ACHLIF_003041"
     CDS             complement(21513..>22096)
                     /gene="yibB"
                     /locus_tag="ACHLIF_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO9607019.1"
                     /translation="QKDESFTNRLEPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGL
                     VKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIG
                     NHVYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLN
                     YLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
BASE COUNT         5623 a         5718 c         5595 g         5160 t
ORIGIN      
        1 tttttatgat gaatgcttaa aacaatattt aatcgacaca catcactgtc attaatgtaa
       61 agcaacatag atcaccaata atacaatgga caactctacc ggcattgatc gctggtcgga
      121 ggaaataagg atttttacat gaacttgaag aagatttttt tcagtgctgt cactgtttca
      181 gttctctgcg cattaacggg atgtgactac attgaagaag gcaagccaga aagtagttta
      241 ttgaaacaac aagaagaaca taacaataaa attgatctac ttgaaaaaca acaagcacag
      301 ctaaagagtc aactggaaac tattcaaaaa caacaaactg gcataataag cagtacaaag
      361 actttaactc acgtaattaa atccgttaaa gatcaacaaa acacttttat ttttaccgag
      421 tttaacccag caaaaacgaa gtatttcatc ctgaacaatg gttcggtggc tttagcgggt
      481 cgagtgttat ccattgacgc cacagagaat ggcagtgtta ttcatatttc actggtcaac
      541 ttattaagta cacctatctc aaatattggt tttaatgcga catggggtgg tgaaaaacct
      601 gtcgatgcca aagagtttgc ccggtggcaa caattacttt tcaacacatc aatgaaatcc
      661 acattgaaat tattaccagg tcaatggcaa gacattaatt tgaccctgaa gggtgtatcg
      721 cccaataacc tgggatatct gaagttagcc atcaacatgg aaaatattca gttcgacaat
      781 cttccttctg ctgaaaatcg gcagaaaaga agcaaaaaaa taaatgcagt tagtacgctt
      841 ataacaagga atagcgagca tcattaatga acaaaatatt taaagttatc tggaaccctg
      901 cgacagggaa ttatactgtt accagcgaaa cggcaaaaag ccgtggcaag aaatctgggc
      961 gcagtaagct gttaatttct gcgctggttg cgggtggaat gttgtcgtcg tttggggcat
     1021 tggcgaatgc cgggaatgac aacggtcagg gtgttgatta cggtagtgga tcagctggcg
     1081 acggctgggt tgctataggc aaaggggcga aagcaaatac ttttatgaac accagtggtt
     1141 ccagtactgc tgtgggttat gacgctatag ctgaaggcca atatagctct gccatcgggt
     1201 caaaaaccca tgcgattggt ggtgcatcaa tggcctttgg ggttagtgca atatcagaag
     1261 gcgatagaag tatagcactg ggtgcctctt cgtattcatt gggccaatac tcaatggccc
     1321 tcggccgtta ttcaaaagca ttgggtaaat tgtctattgc tatgggggac tcttccaaag
     1381 cggaaggagc aaacgccatt gccctgggaa atgccactaa agctactgag attatgagta
     1441 ttgctcttgg cgacaccgcc aatgcgtcaa aagcgtattc aatggcgctg ggagcaagta
     1501 gcgtcgcatc tgaagaaaac gcaattgccc tggggcgtag cagtgtagct agcggtactg
     1561 acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt gcgataggtg
     1621 ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga
     1681 ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag
     1741 gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg
     1801 atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg
     1861 ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca
     1921 gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat ttagacaata
     1981 ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga
     2041 tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg
     2101 tagcaaaaag gcttggagga ggggctgcag tagatgttga tgacggtact gttacagcac
     2161 caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg ggctgcgctc gctgtacttg
     2221 atgaaaacac cctgcaatgg gaccaaacca aaggcaaata cagcgctgct catggtacta
     2281 gtagcccaac tgccagcgta atcaccgatg ttgcggatgg cacgatttca gcctccagta
     2341 aggatgcggt taacggttcc caactgaaaa ctaccaatga cgatgtcgaa gccaacaccg
     2401 ccaatatcgc tactaatacc agcaacattg ccacgaatac ggcaaatatt gccaccaata
     2461 ccaccaatat caccaacctg acggattccg ttggtgacct tcaggctgat gccctgctct
     2521 ggaacgaaac taaaaaggca ttcagtgcag ctcacggcca ggataccacc agcaaaatca
     2581 ccaacgttaa agatgccgac ctgacggctg acagcactga tgctgttaac ggctctcagc
     2641 tgaaaaccac caacgatgct gtggcgacga ataccaccaa tatcgccaat aacacttcca
     2701 atattgccac taacaccacc aacatctcta acctgactga gacggtgact aatcttggtg
     2761 aggatgcgct gaaatgggat aaggacaatg gtgtattcac ggcagctcat ggcaccgaga
     2821 ccaccagcaa aatcaccaac gttaaagatg gcgacctgac gactggcagc accgatgccg
     2881 ttaacggctc tcagctgaaa accaccaacg atgccgtggc gacgaatacc accaatatcg
     2941 ccactaacac caccaacatc tctaatctga ctgagacggt gactaatctt ggtgaggatg
     3001 cgctgaaatg ggataaggac aatggtgtct tcactgcagc tcatggcaac aataccgcca
     3061 gcaaaatcac caatatcctg gacggcacag tcactgcaac cagttccgat gccattaacg
     3121 gtagccagct ttatgactta agcagcaata tcgccaccta cttcggcggc aatgcttctg
     3181 tgaatactga cggtgtgttt accggtccaa cctacaaaat cggtgaaaca aattattata
     3241 acgtcggcga tgcactggct gcgattaact cctcatttag cacgtctctc ggcgatgctc
     3301 tgctttggga tgccaccgca ggtaaattca gtgccaaaca cggtactaat ggtgacgcaa
     3361 gcgtgatcac tgatgtcgca gatggtgaaa tttcagactc cagttctgac gcagtaaacg
     3421 gctcacaact ccacggcgtg agcagttatg ttgttgatgc gctggggggt ggtgccgaag
     3481 tcaatgcaga cggcaccatc actgcgccga cgtacaccat tgctaatgct gattacgata
     3541 atgtcggtga tgccctgaat gctatcgata ccactcttga cgacgctctg ctctgggatg
     3601 cggacgccgg tgaaaatggt gcatttagcg ccgctcacgg aaaagataaa actgccagtg
     3661 taatcactaa cgtcgctaac ggtgcaatct ctgctgccag cagcgacgcg attaacggct
     3721 cacaactcta taccaccaat aagtacatcg ctgatgcgct gggtggtgac gcagaagtca
     3781 acgctgacgg caccatcacc gcatcgactt acaccattgc gaacgccgag tacaacaacg
     3841 tcggtgacgc cctggatgcg cttgatgata acgccttgct gtgggatgag actgccaatg
     3901 gcggtgctgg agcctacaat gccagccatg acggtaaagc cagcatcatc actaatgtcg
     3961 ctaatggcag tattagtgag gacagtaccg atgcagtgaa cggttctcag ttgaatgcga
     4021 cgaatatgat gattgagcag aacacccaaa ttatcaatca gctcgctggt aacaccgacg
     4081 caacctatat ccaagaaaac ggtgcgggta ttaactatgt gcgtactaac gacgacggct
     4141 tagcgttcaa cgacgccagc gcacagggtg ttggcgctac agctataggt tataactctg
     4201 tcgccaaagg cgatagcagc gtagctattg gtcagggcag ctacagcgac gttgatacgg
     4261 gtatcgccct gggtagcagc tctgtttcca gccgagtgat tgccaaaggc tcccgtgaca
     4321 ccagcataac ggaaaatggc gttgttattg gttacgacac cacggatggc gaactgctcg
     4381 gtgcattgtc tatcggtgat gacggtaaat atcgtcaaat catcaacgta gccgatggtt
     4441 ccgaagccca tgacgccgtt acggttcgtc aattgcagaa tgcgattggt gcggtcgcaa
     4501 ccacgccgac taaatacttc cacgctaatt caacggaaga agattcactg gcagtgggaa
     4561 ctgactcgct ggcaatgggt gcgaaaacca tcgtgaatgg cgataaaggt attggtatcg
     4621 gttatggtgc ctacgtggac gcgaatgcac ttaacggcat tgccattggt agcaatgcgc
     4681 aagtcattca tgtcaacagt attgcgatag gtaatggttc tacgaccact cgtggcgctc
     4741 aaaccaatta taccgcctac aacatggacg caccgcagaa ctctgtcggt gaattctcag
     4801 tcggtagtgc ggatggtcaa cgtcagatca ctaacgtcgc agcaggttcg gctgataccg
     4861 atgcggtcaa cgtgggtcag ttgaaagtaa cggatgcgca ggtttcccag aatacccaga
     4921 gcattactaa cctgaacaat caggtcacta atctggatac tcgcgttact aatatcgaaa
     4981 acggtattgg cgacattgtc acctccggta gcaccaagta cttcaagacc aacaccgatg
     5041 gcgtagatgc caacgcccag ggtaaagata gcgttgctat tggttctggt tccattgctg
     5101 ccgctgacaa cagcgtcgca ctgggtaccg gttccgttgc agaggaagaa aatacaatct
     5161 ctgtaggttc ttctactaac caacgccgta tcaccaacgt agctgccggt gttaatgcca
     5221 ccgatgcggt taacgtgtcg cagctgaaat cttctgaagc aggcggcgtg cgctacgaca
     5281 ccaaagctga tggttctatc gactatagca atatcaccct cggtggtggc aacggcagta
     5341 cgactcgcat cagcaacgtc tccgctggcg tcaacaacaa cgacgcggtg aactacgcgc
     5401 agttgaagca aagcgtgcag gaaacgaagc aatacaccga tcagcggatg gttgagatgg
     5461 ataacaaact gtctaaaact gaaagcaagt tgagcggtgg tatcgcttct gcaatggcaa
     5521 tgaccggtct gccgcaggct tatacaccgg gtgccagcat ggcttctatt ggtggcggta
     5581 cttacaacgg tgaatcggca gttgctttag gtgtatcgat ggtgagcgcc aatggtcgtt
     5641 gggtctacaa attacaaggt agtaccaata gccagggtga atactccgcc gcactcggtg
     5701 ccggtattca gtggtaatga tccattaagt tagtgtgact aagggcaaac gccaaattgc
     5761 ctgatgcgtt acgcgcatca ggcaagataa ggcgctccgg gtcaacaatc taagggcaat
     5821 tctctgatga ggattgccct tttctttacc agacatctcc cccctacaag aattggccct
     5881 accaattctt cgcttatctg acctctggtt cacaattccc caattaaaac tcacatcaat
     5941 attgccaata cataacattt agttaaccat tcattgtcat tatccctaca caacacaatt
     6001 ggcagtgcca cttttacaca acgtgtgaca aggagatgag caacagactc attacacgat
     6061 gtgcgtggac tccaggagac ctgcaatgaa tctctggcaa caaaactacg atcccgccgg
     6121 gaatatctgg ctttccagtc tgatagcatc gcttcccatc ctgtttttct tctttgcgct
     6181 gattaagctc aaactgaaag gatacgtcgc cgcctcatgg acggtagcaa tcgcccttgc
     6241 cgtggctttg ctgttctata aaatgccggt cgctaacgcg ctggcctcgg tggtttatgg
     6301 cttcttctat ggtttgtggc ccatcgcgtg gatcattatt gcagcagtgt tcgtctataa
     6361 gatctcggtg aaaaccgggc agtttgacat cattcgctcg tctattcttt cgataacccc
     6421 tgaccagcgt ctgcaaatgc tgatcgtcgg tttctgtttc ggcgctttcc ttgaaggagc
     6481 cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg ctggtcggcc tgggttttaa
     6541 accgctgtac gccgccggac tgtgcctgat tgttaacacc gcgccagtgg catttggcgc
     6601 gatgggcatt ccaatcctgg ttgccggaca agtaacaggc atcgacagtt ttgagattgg
     6661 ccagatggtg gggcgtcagc taccgtttat gaccattatc gtgctgttct ggatcatggc
     6721 gattatggac ggctggcgcg gtatcaaaga gacgtggcct gcggtcgtgg ttgcgggcgg
     6781 ctcgtttgcc atcgctcagt accttagctc caacttcatt gggccggagc tgccggacat
     6841 tatctcttcg ctggtatcac tgctctgcct gacgctgttc ctcaaacgct ggcaaccagt
     6901 tcgcgtattc cgcttcggtg acttgggggc gtcacaggtt gatatgacgc tggcccacac
     6961 cggttacacc gcgggtcagg tgttacgtgc ctggacaccg ttcctgttcc tgaccgctac
     7021 cgtaacactg tggagcatcc cgccgtttaa agccctgttc gcatcgggtg gtgcgctgta
     7081 tgagtgggtg atcaacattc cggtgccgta cctcgataaa ctggttgccc gtatgccacc
     7141 agtggtcagc gaggctacag cctatgccgc cgtgtttaag tttgactggt tctctgccac
     7201 cggcaccgcc attctgtttg ctgcactgct ctcgattgtc tggctgaaga tgaaaccgtc
     7261 tgacgctatc agcaccttcg gcagcacgct gaaagaactg gctctaccca tctactccat
     7321 cggcatggtg ctggcgttcg cctttatctc gaactattcc ggactgtcat caacactggc
     7381 actggcactg gcgcacaccg gtcatgcatt caccttcttc tcgccgttcc tcggctggct
     7441 gggggtattc ctgaccgggt cggatacctc atctaacgcc ctgttcgccg cgctgcaagc
     7501 caccgcagca caacaaattg gcgtctctga tctgttgctg gttgccgcca ataccaccgg
     7561 tggcgtcacc ggtaagatga tctccccgca atctatcgct atcgcctgtg cggcggtagg
     7621 cctggtgggc aaagagtctg atttgttccg ctttactgtc aaacacagcc tgatcttcac
     7681 ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc ttaacgtgga tgattcctta
     7741 atgattgttt tacccagacg cctgtcagac gaggttgccg atcgtgtgcg ggcgctgatt
     7801 gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg agcgccaact ggcgatgcaa
     7861 ctcggcgtat cacgtaattc actacgcgag gcgctagcaa aactggtgag cgaaggcgtg
     7921 ctgctcagtc gacgcggcgg cgggacgttt attcgctggc gtcatgacac atggtcggag
     7981 caaaacatcg tccagccgct aaaaacgctg atggccgatg atccggatta cagtttcgat
     8041 attctggaag cccgctacgc cattgaagcc agcaccgcat ggcatgcggc aatgcgcgcc
     8101 acacctggcg acaaagaaaa gattcagctt tgctttgaag caacgctaag tgaagacccg
     8161 gatatcgcct cacaagcgga cgttcgtttt catctggcga ttgccgaagc ctcacataac
     8221 atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc tgcaatcctc agtgaagcat
     8281 agccgtcagc ggatgtatct ggtgccaccg gttttttcac aactgaccga acaacatcag
     8341 gctgtcattg acgccatttt tgctggtgat gccgacgggg cgcgtaaagc aatgatggcg
     8401 caccttagtt ttgttcacac caccatgaaa cgattcgatg aagatcaggc tcgccacgca
     8461 cggattaccc gcctgcccgg tgagcataat gagcattcga gggagaaaaa cgcatgatta
     8521 tttccgcagc cagcgattat cgcgccgcag cgcaacgcat tctgccgccg ttcctgttcc
     8581 actatatgga tggtggtgca tattctgaat acacgctgcg ccgcaacgtg gaagatttgt
     8641 cagaagtggc actgcgccag cgtattctga aaaacatgtc cgacttaagc ctggaaacga
     8701 cgctgtttaa tgagaaattg tcgatgccgg tagcactggc accggtgggt ttgtgtggca
     8761 tgtatgcgcg tcgtggcgaa gttcaggcag ccaaagcggc ggacgcgcat ggcattccgt
     8821 ttactctctc gacggtttcc gtttgcccga ttgaagaagt cgcgccagcc atcaagcgcc
     8881 caatgtggtt ccagctttat gtactgcgcg atcgcggctt tatgcgtaac gcgctggagc
     8941 gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt ggatatgccg acaccgggcg
     9001 cacgctaccg tgatgcgcat tcaggtatga gcggcccaaa tgcggcaatg cgccgctact
     9061 tgcaggcggt gacgcatccg caatgggcgt gggatgtggg cctgaacggt cgtccacatg
     9121 atttaggtaa tatctcagct tatctcggca aaccgaccgg actggaagat tacatcggct
     9181 ggctggggaa taacttcgat ccgtccatct catggaaaga ccttgagtgg atccgcgatt
     9241 tctgggatgg cccgatggtg atcaaaggga tcctcgatcc ggaagatgcg cgcgatgcag
     9301 tacgttttgg tgctgatgga attgtggttt ctaaccacgg tggccgccag ctggacggtg
     9361 tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc ggtgaaaggt gatatcgcca
     9421 ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt gcgtatgatt gcgctcggtg
     9481 ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct ggcaacagcg ggccaggcgg
     9541 gtgtagctaa cctgctaaat ctgatcgaaa aagagatgaa agtggcgatg acgctgactg
     9601 gcgcgaaatc gatcagtgaa attacgcaag attcgctggt gcaggggctg ggtaaagagt
     9661 tgcctacggc actggctccg atggcgaaag ggaatgcggc atagtcgttt gcgccccctc
     9721 accctaaccc tctccctcag ggagagggga ccgttcggcg ctgtatgtac tccctcactc
     9781 tgaaacgaca ccgcactctt tttttctccc tcgcccctcc ggggtgaggg gaaaaggccg
     9841 cactgctcac caattttctg ctatcctgcc cccgcactaa gggggcagta tgctaaacat
     9901 cgtactttac gaaccagaaa ttccgccaaa tactggcaat atcatccgtc tttgcgctaa
     9961 taccggcttt cgtctgcata tcatcgaacc gatgggattt gcctgggacg ataagcgcct
    10021 gcgccgcgcg gggctggact atcacgagtt taccgccgtt acgcgtcatc atgactttcg
    10081 cgcgttcctc gaagcagaaa atccccagcg cctgtttgcc ctcactacga aaggtacgcc
    10141 tgctcacagc gccgtaaact atcaggatgg cgattatctg atgttcggcc cggaaacacg
    10201 cggcctgcca gcgagcattc ttgatgccct gcccgctgaa caaaaaattc gcattccaat
    10261 ggtgccggac agccgcagca tgaacctgtc caatgcggtg tcggtagtgg tgtatgaagc
    10321 ctggcggcag ctgggatatc cgggagcgtt gttgagagat tagttactgt atgccggata
    10381 gatgattaca tcgcatccgg cacgatcaca gcacatcaga tcccatcccc atactcaaat
    10441 gtatggttaa taccgttgaa atgctggtcc atatccattg atggcttatc gctgtctggt
    10501 ttaccgacaa tacgagccgg aacaccagcg gcggtagtat gcggcggcac cggttgcagc
    10561 accacggaac ctgcgccaat cttcgcgccg cgaccaactt caatattgcc gagaattttc
    10621 gcgcccgcgc caatcatcac accttcacga attttcgggt gacggtcacc accagattta
    10681 cccgtaccgc caagcgtcac agattgcaga atcgatacgt cgttttcaat taccgccgtt
    10741 tcaccaacga cgatgcccgt ggcgtggtcg agcatgatac cgcgaccaat ttttgctgcc
    10801 gggtgaatat cgacctggaa cgtcacagaa acctggtttt gcagaaagat tgccagtgcg
    10861 cgacgcccct gattccacaa ccagtgaccg atgcgatagg cctgcaaggc atgaaaaccc
    10921 ttcaggtata acaacggggt tgagtattta tcgactgccg ggtcgcgggt acgcaccgcc
    10981 tgaatatcac aggccgcaga ggcgatcatt tccgggtcag cggcgtaggc ttcttcaacc
    11041 acttcacgga tagcaatagc aggcataatt ggcgatgaca gcttgttcgc cagcatgtag
    11101 ctcagggcac tgccaaggtt ttcgtgcttg agtagcgtcg cgtggtaaaa actggccagc
    11161 attggctcac agtccgccag cgttctggct tcggctttaa tattgttcca gacaatttcc
    11221 agttcttcac acgacattgc ttactccaca cgatgagata atgaccggcc cgttctgcgc
    11281 gggccgggtc atagcggtaa caaaggttcc ctggggttag tggctgctgc gctcgtcctt
    11341 gcgtgcacga cctagtaaag tcaatgctgc ctcgcgcgcg ttttttccgc aatataatac
    11401 ttgataaatt tcctcggtta ttggcatttc aacgccgaag cgatgcgcca gttcgcggac
    11461 ttctttcgta ttgcggtagc cttccaccac ctgaccaatc ttctcctgcg cgctttgtac
    11521 atccatgccc tgaccgagca tcatgccaaa acggcggtta cgcgactggt tgtcggtaca
    11581 ggtaagcacc agatcgccca gccccgccat gcccataaag gtggcaggat cggcacccag
    11641 cgccgcacca agacgcgaca tttcagccag cccacgggtg atcagcgccg tacgcgcatt
    11701 cgcaccaaaa ccgataccgt cagacatccc cgcgccaatg gcaatgacgt ttttcaccgc
    11761 gccgccaagc tgcacgccaa tgaaatccgg gttgctgtaa acgcggaaac ttttgccaca
    11821 gtgcagcaat tgttggagat catcggcaaa ggtctggtcg gtagaggcca acgaaatagc
    11881 cgtcggtaaa cctgccgcca gttctttcgc aaacgttggg ccagagataa ccgccagcgg
    11941 aatttgatcg cctaaggcct cacgcgccac gtcctgtaac agacgtccgg tttccgcttc
    12001 cagccctttg gtcgcccaca ccagacgcgc atcaggacgc atcagcggtt taatctggcg
    12061 cagcacttca ccaaagacat ggctgggtac gacgacgaga atattacggc tggctgccag
    12121 cgcagtggcg agatcgcttt caagatggag cgtatcggga aaaggcacat cggggagaaa
    12181 cgcggcgtta cagcggtcgc gttcaagcgt tgcgatatgt tcagggtcat ggccccagag
    12241 gacaacctcg tggccatttc ttgccagggt aatggcaaga gcggtgccgt acgagccggc
    12301 accgatcaca gtcattgaag cattacgttg gttcatcagg catcctgatg ttcttcagta
    12361 ccttcgccag cctgctgctg caaatagttc atgaacagcg catcgaagtt aaccggcgca
    12421 aggttcagtt gcgggaatgt accgcgggat accatgctgg tgatgcactc acgagcatac
    12481 gggaacagaa tgttcgggca gtatgcgccc aggcaatgtg ccatctgggt gccttcgata
    12541 cccgcgatgg agaaaatacc gccctgctga acttcacaca ggaacgcggt ttcttcgccc
    12601 agggaggccg ttacggtaac acgcagtacc acttcgtata cgtcatctgc cagttgggaa
    12661 gatgccgtat ccagatcaag tttaacttct ggttgccaat ctttctggaa aacgtgcggc
    12721 gcgttcggcg cttcgaaaga gatatccttg gtataaatac gttggatctg gaaagtcatt
    12781 tcagtgttgt tttgttctga catgtgtaga aaacccttaa gtgttgtcct taaatactgc
    12841 gcaatgtaat gccaacgttc acacgttatt tcagcagggg atccagtcca ccacgtgcat
    12901 ccaatgcata caagtcatca cagccgccaa tgtgctgtgc gtcaataaaa atctggggaa
    12961 ccgtggtgcg accgctgcgt ttgatcatct cttcacgctt ggcggcattg ccatcgatcg
    13021 gcaactcctg aaaactcacg cccttgctgc tcagcagtgc ttttgcacga tggcaatacg
    13081 ggcaggtttc tttggtatag atttcaacat tggccataat ttagctcctg tttttttacc
    13141 ctgaaatttc atgttgcagg gcggctgcaa aatggagtgt ccccagaagc ttataacaat
    13201 aagtcgctgg gggcctaatt gcagctaacg gcctgcatca tgaaagacga caggtaaatt
    13261 atttgccgcg caccagaggc aagttttcgc cagcccagcc agcaacgccc tctttcagca
    13321 cgaatacttg cgcaaaacca gctttcgtca gcgcgtttgc aggctcctgg cactgcatgc
    13381 cagaaccgtc taccacgata accggtttgt ctttgtgctt ctcaagctca ccaacattgt
    13441 tggctttgat ttcactcggc aacagattga tagaacctgc gatatggcct ttacggaagt
    13501 catcacgctg acgtaaatcc acaaccacag cgtcttcttt gttgatcaga cgcgtagctt
    13561 caccacgagt aatcaccttc actttcgagg tcaggctctt aaacgtagtc acaagaaccg
    13621 ccaccagtaa cgcgatccag gcgatactca gtatgggatg acggccaaca aattgcataa
    13681 tttcttgcat ggggggtaac aactcccgac gtagtgatta aaaaaaccag gaaaggagta
    13741 tacctgcgcg gtgcggcaaa tacagccagc gcgttaactg gaatgcaatt ttgcggggca
    13801 cgacgaaaaa aaagcgcaaa tctggcatcg gcttgccgcg agcggtcgta tattttgatc
    13861 ttcagaggct attttatcga ttcagctgta gtaaaattac gcaaattttg actcttgagt
    13921 atgaggttgt cgcaatgtcg gtttctaaaa aacctatggt actggtgatt ctggatggct
    13981 atggctatcg tgaagaacag caggataacg ccatttttag tgctaaaacc ccggtaatgg
    14041 atgcactgtg ggccaatcgt ccgcataccc tgatcgacgc ttccggtctg gaagtcggtc
    14101 tgcctgaccg tcagatgggt aactccgaag taggccacgt taacctgggt gccggccgca
    14161 tcgtgtatca ggacctgact cgtctggacg ttgaaatcaa agatcgcgct ttctttgcta
    14221 atccagtgct gactggcgcg gtagataaag cgaaaaacgc aggtaaagca gtacacatta
    14281 tgggtctgct ctccgcaggc ggcgtacaca gccacgaaga tcacatcatg gcgatggtag
    14341 aactggcagc tgaacgcggc gcagaaaaaa tctacctgca cgcattcctt gacggtcgcg
    14401 acaccccgcc gcgcagtgct gaatcctcgc tgaaaaaatt tgaagagaaa tttgctgcgc
    14461 tgggtaaagg ccgcgtagcg tccatcattg gtcgttacta cgcgatggac cgcgataacc
    14521 gttgggatcg cgttgaaaaa gcctatgacc tgctgactct ggcgcagggt gagttccagg
    14581 ccgatactgc cgttgctggt ttgcaggcag cttatgctcg cgacgaaaac gacgagttcg
    14641 tgaaagcgac cgttattcgt gcagaaggtc agccagatgc ggcaatggaa gacggcgatg
    14701 cgctgatttt catgaacttc cgtgctgacc gcgcgcgtga aatcactcgt gcattcgtga
    14761 acgctgattt cgatggcttc gcgcgtaaga aagtggttaa cgtcgatttc gtgatgctga
    14821 ccgaatacgc cgctgacatc aaaactgcgg ttgcttaccc acccgcttcc ctggttaaca
    14881 ccttcggcga gtggatggcg aaaaacgaca aaactcagtt gcgtatttcc gaaaccgaaa
    14941 aatatgccca cgttactttc ttcttcaacg gtggcgtaga agagtcgttc aaaggcgaag
    15001 atcgcattct gatcaactca ccgaaagtgg ctacctacga tctgcaaccg gaaatgagct
    15061 ccgcagagct gaccgaaaaa ctggttgcgg ccatcaagag cggcaaatac gacaccatta
    15121 tctgtaacta tccgaacggc gacatggtag gtcacaccgg ggtgatggaa gcggcggtta
    15181 aagcggttga agcactggat cactgcgtgg aagaagtcgc gaaagcggtt gagtccgtgg
    15241 gtggacaact gctgatcacc gctgaccacg gtaacgctga acagatgcgc gatccggcaa
    15301 cgggtcaggc acacacggca cacaccaacc tgccagttcc gctgatttat gttggtgata
    15361 agaacgtgaa agcggttgaa ggcggcaaac tttctgacat cgcgccgacc atgttgtcgc
    15421 tgatgggtat ggaaatcccg caagagatga ctggtaagcc gctgttcatc gtggaataat
    15481 ccctccccat gaggggaaag gcgattaata ccatgacacg ggccgtgaaa ccgcgcaggt
    15541 ttgcaatcag gcccatcatc tacgccagcg ttcttagcgc tggcgtattg ttgtgcgcct
    15601 tttccgccca cgcggatgag cgtgaccaac tcaaatctat tcaggccgat atcgccgcaa
    15661 aagagcgcgc ggtacgccaa aagcaacaac aacgcgcaag cctgctcgca caattgaaaa
    15721 agcaggaaga agcgatctct gaagccaccc gtaagctgcg cgaaacgcaa aacacgctca
    15781 atcaactcaa taaacagatt gatgagatga acgcgtcgat tgccaaactg gagcagcaaa
    15841 aagccgccca ggagcgcagc ctcgccgcgc aactggatgc cgcattccgt cagggcgagc
    15901 ataccggtat tcagctaatt ctcagcggtg aagaaagcca gcgtggacag cgtttacagg
    15961 cttatttcgg ctatctcaac caggcgcgac aagaaaccat tgcccagttg aagcaaacgc
    16021 gtgaagaagt cgccatgcag cgtgctgaac tggaagagaa acaaagcgag caacaaacgc
    16081 tgttatatga acagcgcgcc caacaggcga aactgactca ggctctgaac gagcgtaaaa
    16141 agacgctggc ggggctggaa tcttccatcc agcaaggtca gcagcagcta agtgaactgc
    16201 gtgccaacga atcccgtctg cgtaacagca ttgcccgtgc ggaagccgcg gcgaaagcgc
    16261 gtgctgaacg agaagcgcgt gaggcccagg cggttcgcga ccgccagaaa gaagcgacgc
    16321 gcaaaggcac cacctataaa ccgaccgaaa gcgaaaaatc gctgatgtcc cgaactggtg
    16381 gcctgggtgc gccgcgtggt caggcattct ggccggttcg cgggccgacg ctgcatcgct
    16441 atggtgaaca gctacagggc gaactacgct ggaaaggaat ggttatcggt gcctctgaag
    16501 gtactgaagt taaagcgatt gccgacggtc gggtgattct ggctgactgg ctgcaaggtt
    16561 acggtctggt ggtggtggtt gagcacggta aaggcgacat gagtctttac ggctataatc
    16621 agagcgcact ggtgagcgtt ggttcgcagg ttcgcgcggg ccagccaatt gcactggtgg
    16681 gcagcagtgg cggtcagggt cggccttcac tctatttcga aattcgccgc cagggtcagg
    16741 cggtcaatcc acagccgtgg ttgggaagat aagttttgtt tccatttcgt cgtaacgttc
    16801 ttgcatttgc cgctctgttg gcgctctcct cccccgtact tgctggcaaa cttgccatcg
    16861 tcattgatga ttttgggtat cgcccgcaca acgaaaacca ggtgctggcg atgccctccg
    16921 ctatctccgt cgctgtatta cccgattcac cgcacgccag agagatggcg accaaagcgc
    16981 ataacagcgg gcacgaagtg ttgattcatc tcccgatggc accactgagt aaacagccgc
    17041 tggagaaaaa tacgctgcgc ccggagatga gcagcgacga aattgagcgc attattcgta
    17101 gtgcggtcaa taacgtgccc tatgccgtgg ggatcaacaa ccacatgggt agcaagatga
    17161 cctctaacct gtttggtatg cagaaagtga tgcgggcact ggagcgttac aatctttact
    17221 tcctcgacag cgtaaccatc ggtaataccc aggcgatgcg cgccgcgcaa ggtactggcg
    17281 tgaaggtgat caaacggaag gtgttcctcg acgattcgca gaacgaagcg gacatccgtg
    17341 tgcaatttaa tcgcgcaatt gatctggcgc gtcgcaacgg ttcaaccatt gccattgggc
    17401 atcctcaccc ttcaacggta cgcgtgctac aacagatggt ttataacctg ccgccagaca
    17461 ttacgctggt gaaagccagc agcttgctga atgaaccgca ggttgatact tctacaccgc
    17521 cgaaaaacgc tgtgcctgat gcaccgcgta atccgttccg tggcgtgaag ctgtgcaaac
    17581 cgaagaaacc gatagaacct gtttatgcta atcgcttctt tgaagtatta agcgaaagca
    17641 tcagccagag cacgctgatc gtttacttcc agcatcagtg gcaaggctgg ggcaaacagc
    17701 ccgaagcggc gaagtttaac gctagcgcaa attaaaggcg ctacgcgtaa tgcgatggtg
    17761 cgaccgcgtt ttgtcgcgcc attgccataa gcggaaagac cacagtaacg cctggtaacc
    17821 gaccttcacg ctgcgcacat tggtaatcag gcgcttatac ataccggaag taaatatctc
    17881 ggcaatcatc cgttggcggg taagaatatc cggctctttg cgcaccgcat ggcaaacacg
    17941 caatgcttcg taagttattt gctgatgaaa ttccggataa atcgtaattt tgtcggcata
    18001 atttcgattt aatttctcca gcaggcgggt aatcttaata tagtgacgtt gataattaag
    18061 gtttttattc ccttgtctat gcaaccgact cactgacgta ttatgcagat aatatttata
    18121 taatgattgc tcggtatatc gcgcacgcag cgcgttaaac atgaattctg ttgtccagac
    18181 aatatcctga tgatgtaatc cggcaataaa tttaatgtta tttttaacaa taacatcacg
    18241 gcgataaacc cccatccaga caacgtgagt ccaacggcgc gaagaaagcc ccatccgcag
    18301 ccagtccggg ccggttaata cgccggttga gcgaaggcga tcgctgggga tggattgcca
    18361 ggtttctccc gtttcacgaa aacaccagtc agcgttgcac tgcgccacgt cgaggtcatc
    18421 ctctaacgcc atggtcatca gcgtttcgta catggtggga tagacctcat cgtcagcatc
    18481 gacaaaagcg acatatttgc ccgtcgccac ctcgatccca cgattacgcg ctaccgatgc
    18541 gccagcattc gcctgatgca acaaacgaac gtgcggatag ttttctgcgt aatgctttgc
    18601 tatttcaaca gaattatccg ttgaaccatc gttaataata atgatttcca gagcagtcca
    18661 ggtttgcgta attaaagatt ccatacaagt gcggaaatca tcgcccgcat tatataacgg
    18721 aataataaca ctaagtttat tggtgctgtt catcataaat taccgacgac ctctgaagtt
    18781 atcctgacgt tttacaggct ttgtattaag aaactattaa gcctgtgaga tattattttt
    18841 acaattattt ttacaggtga gagagtcact tcagggtaga gagataatga gagcagctat
    18901 ataagagatc agcgcaatta cgatatcttt attacactga caggatttta catatttaac
    18961 ggattatcct caggcaaaag ggctggaata ccagcccttg ttcgtgttaa tcccaactca
    19021 gaattacttt cccggactgg cccgaacgca tagcgtcaaa gcccttctgg aaatcatcga
    19081 tagagaaacg atgggtaatg atcggcgaga gatccagacc tgactgaatc agcgccgcca
    19141 tcttgtacca ggtttcaaac atctcacgac cgtaaatacc tttaatgaac aagcctttaa
    19201 agattacttt ggtccagtcg atagacatat cggacggtgg aatacccagc atcgcaatac
    19261 gaccgccgtg gttcatggta tcgagcatgg tacgaaacgc tggcggcgcg ccggacattt
    19321 ccagaccgac atcaaagcct tcggtcatgc ccagttcagc catcacatca ttaaggtttt
    19381 ctttcgcgac gttaaccgca cgggtgatac ccattttgcg cgccagctca aggcggtatt
    19441 cgttaacatc ggtgatcacc acattgcgtg caccaacgtg tttcgccacc gccgcagcca
    19501 taatacctat cggacctgca ccagaaacca gcacatcttc gcccaccaga tcgaacgaca
    19561 gcgcggtatg cacggcgtta ccgaagggat caaaaatgga agccaggtcg tcggagatat
    19621 tgtctgggat tttgaaggcg ttgaacgccg ggatcaccag atattcggcg aagcagcccg
    19681 gacggttaac gccgacgccg atcgtgttgc ggcacagatg ggtacgaccg ccacgacagt
    19741 tgcgacagtg accgcaggtg atatggcctt cgccagaaac gcgatcgccg attttgaagc
    19801 ctttcacttc ctgaccaata cctaccactt caccgacata ttcatggcct acgaccatcg
    19861 gcaccgggat ggttttttgc gaccactcat cccagttata gatgtgaacg tcagtcccgc
    19921 agatggctgt tttacggatt ttaatcagca gatcgttatg cccgagttcc ggtacaggaa
    19981 cgtcggtcat ccagatgccc tcttccgctt tcagtttgga taacgctttc atctcacatc
    20041 ctcaggcgat aacgcccagt tgtttaccaa tacgcgtgaa tgcttctact gcacgcgtaa
    20101 tttgctcagg ggtatgcgcc gcagacatct gggtacgaat acgcgcctga cctttcggaa
    20161 cgaccggata gaagaaaccg gtgacgtaaa tgccctcttt ttgcagctca cgggcaaatt
    20221 tctgcgccac tactgcatcg ccaagcatga ccggaataat ggcatgatcg gctcccgcca
    20281 gggtaaagcc cgccgccgac atttgctcac ggaactggcg cgcgttcgcc cacagacggt
    20341 cacgcagttc gctgcccgct tcgaccatct ccagtacttt gatggacgcg gcaacaatgg
    20401 ccggtgccag cgagttggag aacaggtacg gacgagaacg ctggcgcagc cactcaacca
    20461 cctctttgcg cgccgcggta taaccaccag aagccccgcc cagcgcttta ccaagcgtac
    20521 cggtgataat atcgacccgg cccatcacat cgcagtattc atgggaacca cgaccatttt
    20581 caccgacaaa accgaccgcg tgggagtcgt ctaccatcac cagggcatcg tatttatctg
    20641 ccagatcgca aacgcccttc agattggcaa tcacgccgtc catcgagaat acaccatcgg
    20701 tggcaatcag cacatgacgc gcaccggctt cacgcgcttc tttcagacga gcttcaagct
    20761 cctgcatatc gttgttggca tagcgatagc gtttagcttt acacagacgc acaccatcaa
    20821 taatagacgc gtggttcagt gcgtcggaga taatggcgtc ttccgcgccc agcagcgttt
    20881 caaacaggcc accgttagca tcaaagcagg aagagtagag aatcgcatct tccatcccca
    20941 ggaaggccgc cagtttttgt tcaagctctt tatggctgtc ctgagtgccg caaataaaac
    21001 gcaccgaagc catgccgaaa ccgtgagaat ccattcccgc ctttgccgcc gcaatcagat
    21061 caggatgatt cgccagcccg agatagttgt tggcacaaaa gttaatgacg tggcttccat
    21121 cagccacagt gatatctgct tgctgcgcag acgtaataat gcgctcttct ttaaacaacc
    21181 cttccgcccg tgcggtttcc agatcgttgg ttaactgctg ataaaattct ccacgcattg
    21241 cgattctcca gacagggcaa attccagcac atattaccca aacttatagg tcgcgacgag
    21301 atgacgcgtt aacacttctg caaaattcag gataaataac gtgtaacttg atgtctctca
    21361 acacctacag atagtagaaa aactgtacag ttttggctgg gcataatgat gattgatttt
    21421 actgcattga tataaaaaga aaaaatgatg ccaaagcatc atttttccta tctcagttgg
    21481 tatcaaaacg ctacccacta atggagggaa acctattttc ttgaaaggaa aattctcagc
    21541 gcctgcattt ttgcccctaa agtgttactc ctgaagcagc gaaacaaatc gaaccatttt
    21601 cctctcccca gatagtttag gttgaagaga tcggggcgtt tgtaatagca catgacaaaa
    21661 atgccctggt catcatccac aatgttatta tttagtgtta ttttttgact ttccagaact
    21721 aatttataga attctttcca tttatgctgt gaaccaacaa ttgcgccccc aataatatat
    21781 acatgattgc caatcatgaa atcaaaaacc tgttgttgag atgtgacagt aagccctttt
    21841 tttatagtga aaagatgcat cttactttca tcaaatggga aatcccaaat tttcaatcct
    21901 cgagttacat ttggcttacg gcaatagcca aaatctatcc acgcaacaag aggcgttttc
    21961 actaagccca tgttgatagc tttatttaca aaataggcct taaggttaca taccaaaaca
    22021 tactctggtg accagtactc tgggtttttt aattgtcgag gctcaagtct atttgtaaac
    22081 gactcatctt tttgaa
//
LOCUS       DAZGOB010000074        21637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGOB010000074 DAZGOB010000000
VERSION     DAZGOB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.074"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..306
                     /gene="cybC"
                     /locus_tag="ACHLIF_003042"
     CDS             <1..306
                     /gene="cybC"
                     /locus_tag="ACHLIF_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO9607020.1"
                     /translation="NMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLE
                     DKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(351..815)
                     /gene="nrdG"
                     /locus_tag="ACHLIF_003043"
     CDS             complement(351..815)
                     /gene="nrdG"
                     /locus_tag="ACHLIF_003043"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO9607021.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(974..3112)
                     /gene="nrdD"
                     /locus_tag="ACHLIF_003044"
     CDS             complement(974..3112)
                     /gene="nrdD"
                     /locus_tag="ACHLIF_003044"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO9607022.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(3506..5161)
                     /gene="treC"
                     /locus_tag="ACHLIF_003045"
     CDS             complement(3506..5161)
                     /gene="treC"
                     /locus_tag="ACHLIF_003045"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO9607023.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(5211..6632)
                     /gene="treB"
                     /locus_tag="ACHLIF_003046"
     CDS             complement(5211..6632)
                     /gene="treB"
                     /locus_tag="ACHLIF_003046"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO9607024.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(6751..7701)
                     /gene="treR"
                     /locus_tag="ACHLIF_003047"
                     /pseudo
     CDS             complement(6751..7701)
                     /gene="treR"
                     /locus_tag="ACHLIF_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            7883..7936
                     /gene="mgtL"
                     /locus_tag="ACHLIF_003048"
     CDS             7883..7936
                     /gene="mgtL"
                     /locus_tag="ACHLIF_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO9607025.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8077..10773
                     /gene="mgtA"
                     /locus_tag="ACHLIF_003049"
     CDS             8077..10773
                     /gene="mgtA"
                     /locus_tag="ACHLIF_003049"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO9607026.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(10956..11309)
                     /gene="yjeI"
                     /locus_tag="ACHLIF_003050"
     CDS             complement(10956..11309)
                     /gene="yjeI"
                     /locus_tag="ACHLIF_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO9607027.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11447..13093)
                     /gene="groL"
                     /locus_tag="ACHLIF_003051"
     CDS             complement(11447..13093)
                     /gene="groL"
                     /locus_tag="ACHLIF_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO9607028.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13137..13430)
                     /gene="groS"
                     /locus_tag="ACHLIF_003052"
     CDS             complement(13137..13430)
                     /gene="groS"
                     /locus_tag="ACHLIF_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO9607029.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13706..14962
                     /gene="yjeH"
                     /locus_tag="ACHLIF_003053"
     CDS             13706..14962
                     /gene="yjeH"
                     /locus_tag="ACHLIF_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO9607030.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(14978..15454)
                     /gene="fxsA"
                     /locus_tag="ACHLIF_003054"
     CDS             complement(14978..15454)
                     /gene="fxsA"
                     /locus_tag="ACHLIF_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO9607031.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            15791..17227
                     /gene="aspA"
                     /locus_tag="ACHLIF_003055"
     CDS             15791..17227
                     /gene="aspA"
                     /locus_tag="ACHLIF_003055"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO9607032.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17345..18646
                     /gene="dcuA"
                     /locus_tag="ACHLIF_003056"
     CDS             17345..18646
                     /gene="dcuA"
                     /locus_tag="ACHLIF_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO9607033.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18762..19100
                     /gene="cutA"
                     /locus_tag="ACHLIF_003057"
     CDS             18762..19100
                     /gene="cutA"
                     /locus_tag="ACHLIF_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO9607034.1"
                     /translation="MLDEESSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19076..20773
                     /gene="dsbD"
                     /locus_tag="ACHLIF_003058"
     CDS             19076..20773
                     /gene="dsbD"
                     /locus_tag="ACHLIF_003058"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO9607035.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEVKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20810..21385
                     /gene="yjdC"
                     /locus_tag="ACHLIF_003059"
     CDS             20810..21385
                     /gene="yjdC"
                     /locus_tag="ACHLIF_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9607036.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21492..21567
                     /locus_tag="ACHLIF_003060"
     tRNA            21492..21567
                     /locus_tag="ACHLIF_003060"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21525..21527,aa:Phe,seq:gaa)
BASE COUNT         5046 a         5629 c         5578 g         5384 t
ORIGIN      
        1 aatatggaaa ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa
       61 gtcaaagacg cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg
      121 ccgaagctcg aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc
      181 gacattctgg tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaagtaaaa
      241 gaagcgcagg ctgctgcaga gcaactgaaa acgacccgca acgcctatca ccagaagtat
      301 cgttaattcc tcatttccct gttgcctgca ctcaggtaac agggaactta tcatcgcaaa
      361 tgatgcacca cctgattgct gctgccgcgc cagatcaggg aagggtcttt taaatcctgc
      421 acaaatttgc cgtcgaccag tacgttaatc aaatcaacaa cctgcatttg cgcagcgttg
      481 agttcgtcga gtttatagcc tgtccacacc cagatgtctt tacccggaca ctcggcgcgg
      541 atgcgtttta ccagtttcag aatatccggc acgttttgcg gatgcagcgg atcgccgccg
      601 gagagggaaa taccctggcg tttgatacga gtgtcattca gatcgttaat aatctggtct
      661 tccattgctt tagtaaatgt ctgaccggaa tttacccgcc atgtgctttt gttatagcaa
      721 ccggggcatt catgaacaca cccggagaca aacagggtgc agcgagtgcc ggggccgttg
      781 acgatgtcga caggatagta ctgatgataa ttcattgttt cgcctggctt atcagtccgc
      841 gtataggatg catccggtaa tcaacgcctg atgcgacgct gtcgcgtctt atcagaccta
      901 caatcgttgt tgccatgtag gccggataag gcgtttacgc cgcatccggc atttgcttaa
      961 cgtagagtga agattaacct atctgcccat tccccaaatg tttaacgcgg cgcttaactt
     1021 cttcctgctt accggcgtta aatggacgtg catccgggct acctaaatat ccgcacacgc
     1081 ggcgagtcac cgacacacgg gaggcgtcat ggttaccaca tttcgggcaa gtgaagcctt
     1141 tgctggtgca ctcgaactca ccggtaaagc cacactcgta gcactcatca atcggtgtat
     1201 tggtgccgta atacggtaca tgctgatagc tgtaatccca gacatcttcc agcgccttca
     1261 ggttgtgctg aatgtttgga tactcgccgt agcaaatgaa accaccgttc gccagcggcg
     1321 ggtaaggcgc ttcaaagtcg atcttgtcgt acgggttcac cttcttctcc acatcgaggt
     1381 ggaaactgtt ggtgtagtaa cctttatcgg tcacgcccgg caccacgcca aactcagcag
     1441 tatcgagacg gcagaagcgg tcgcacaggt tttcactcgg cgtgctgtag agactgaaac
     1501 cataacccgt ttcttctttc cactgatcca ctgcctggcg cagacgttca acaatggcga
     1561 tacctttcgc gcgaagctgc tcgttgtcgt aaacatgctc gccgccgaac agcgcgttaa
     1621 tggtttcgtg gatgccgatg taacccagcg aaatagacgc acgaccgttt ttgaagattt
     1681 cagaaacatc atcgtcagca ttcagacgta cgccacaagc accttccata tagaggatcg
     1741 gggccacgcg cgctttcacg ccttcgagac gagcgatacg ggtcatcagc gccttacgtg
     1801 ccagcaccag acgttcatcc agcagcttcc agaaggtggc ttcatcgcct tttgcttcca
     1861 gagcaatacg cggcaggttc aggctgatca cgccgaggtt gttacgacca tcgtggatct
     1921 gctcgccgtt ttcattttcc cacacgccga ggaagctgcg gcagcccatc ggggttttaa
     1981 acgaaccggt gactttcact acctgatcgt agttcaggat atccggatac atgcgcttgc
     2041 ttgcgcactc cagcgccagc tgtttgatgt cgtagttcgg atcgcctttt ttatggttca
     2101 gaccatcgcg aatcgcaaac accagtttcg ggaacaccgc agttttacgg tttttaccga
     2161 ggcctgcgat acggttacgc aggatcgatt cctgaatcag gcgcgattcc cagctggtgc
     2221 ccagaccaaa accaaaggtt acaaacggcg tctgaccgtt ggcggtgtgc agggtgttta
     2281 cttcgtactc cagcgactgg aaggcatcgt agcactcttt gatggtacga gagtttgcgt
     2341 agccttcggc gtccgggatg ttccactctt ctgcggtctt gcgatgtttg ttgtagctgg
     2401 cggtaacaaa cggcgccagc acttcatcga tacggttaat ggtggtgccg ccataaatat
     2461 ggctggcgac ctgagcaata atctgcgcag ttaccgcggt tgccgtagag atcgacttcg
     2521 gcggttcaat ctcggcgttc cccatcttaa agccctgggt cagcatgcct ttcaggtcga
     2581 tcaacatgca gttgaacatc gggaagaacg gcgagtaatc gagatcgtga tagtgaatat
     2641 cgccacgctc atgtgcctgc accacgtcac gcggcagcag gtgctgacgt gcatagtgtt
     2701 tagccacgat cccggccagc aggtcgcgct gggttggaat caccttgctg tctttgttgg
     2761 cgttttcgtt gagtaacgag gcgttggtct gctcgaccag accacggatc tcctggttca
     2821 ggcgaccgcg tttttcacgt tcaatgtcgc gatcgtgacg gtactcgatg taagcacgag
     2881 ccagttgttt gtatggaccc gacatcagct ggttttcaac tgcggtctgg atctcgttga
     2941 tatccacctg gttgcggccc tgcatctgct cgctgacaac cgcggcaaca gtggcgcaat
     3001 aatcggcatc atcgacttcc gctgctttag ctgcacgcag aatcgcttct ttgatgcgct
     3061 ctgatttaaa cggcactttg cagccgtctc gtttcatcac atgcggtgtc atgatcgctc
     3121 catattttta agaacaggtt atccacagaa attgggaaag gcattttccg gttttttcgt
     3181 ttcgttttcc ggtgctttcc gcaatcgcca tccgcttatc cacaacgccg cacctttttc
     3241 gtggggagct gtagtagcaa ttatagtcga ttaatacaac atattgggtt gggacgcatt
     3301 ttaaagtcta tatatagtgc tttgcatcaa ggatgtttta actttttttg atgtagctca
     3361 aagtaaaaag cagagcgtac ggatgacggg cgctacagcg ttatgtaaat tttttaatga
     3421 atttgctggt tgaaaaatca acaaaaacaa catgctgaca gacaaaaccc cggaatgacc
     3481 ggcattaccg gggcttaggg aagatttact tctgtaacca ccagacagcc tcaaaaggcc
     3541 gtaaattcat ggcacagggt tgtggtgagg cttcttcgta gttatgcatc acaagctgcc
     3601 agttgccgcg catttgccct ggctgccagg gttggatctc acggctaagg ttggcaatga
     3661 ccagcaaggt ttgccccttc cattcacggc gatagcacca caatacaggg ctgtttggca
     3721 gcagatcctg gtaattgccc catgtcagga tggcttcttg cttacgcagt gcgattaact
     3781 tttggtaggt gtaaaacacc gaggagtcat cggccagcgc ggcttctacg ttgatttgtt
     3841 gatagttatc gcccaggcca atccacggtt cgccagccgt aaacccggca ttatcgccgt
     3901 tgctccattg catgggcgtg cggctgttgt cacgggattt actggcgagg attgccaata
     3961 actcgtcggc atcacgacca tcgttgcgca gctcggcaaa catattgagg ctctccacgt
     4021 cgcgatagtc agtaatgcgc gtgaaatgcg ggttggtcat gccaatctct tcgccttggt
     4081 agatatacgg cgttccctgc atgccatgca gcaccatcgc cagcattttt gccgcaggca
     4141 cgcggtattc accttcatca ccaaagcgag aaacaatgcg cggctgatcg tggttacacc
     4201 agaacaaggc attccatgcc acgttgtgca ttccttgttg ccagtggcgg aacaatgttt
     4261 tcaacgccac aaagtcaggt ttagccagcg tccatttttc accaccggga taatcgacct
     4321 tcaggtgatg aaaattaaag gtcatcgaca attcactgcc tgtcagtgcc gcgtatcgct
     4381 ggcaatgctc aaggctggtg gaggacattt cacctacggt cattaacccg cgtggcgtaa
     4441 acacatcgcg gttcatctcg tgcaaaaact cgtgtgctcg tggcccgtcg gtgtagaagc
     4501 gacgcccgtc gccatccagg tcttcaggga aacgcgggtc tttggagatc agattcacca
     4561 catccaggcg caacccgtcg accccacgat cggcccagaa ttcacagact tttttcagct
     4621 ctgcgcgtac cgctggattc tcccagttga gatccgcctg ttctggtgca aagagatgca
     4681 aatagtactg ttcgctttcc gcatgccagc gccacgcact accgccaaat tttgaacgcc
     4741 agttgttcgg tggcgtttct ggttcgccat cgcgccagat ataaaactgg cggtaagggc
     4801 tttctttgtt cagcgcctcg cgaaaccagg catgttgggt agaggtatgg ttaaacacca
     4861 tatcgagaat gatacgaatc ccgcgcgatt ttgcctgcgt caccagttcg tcaaaatcgt
     4921 ccagcgtgcc gtagatggga tcaatcgccg tatagttcgc tacgtcgtaa ccgttatcga
     4981 cctggggaga gatataaaag ggggttagcc agatggcatc aacgcccagt ttatgcagat
     5041 agtccaggcg ttggataacg ccacgtaaat cgccggtgcc gctacccgtg gtgtcctgaa
     5101 aactctttgg ataaatctgg tagataacgc cgttttgcca ccagtgggga agattagtca
     5161 ttacgttatt cctgcaaatg cgagggggcg caattgcgcc ccgaagaaaa ttaaacaata
     5221 tccagcgtgc ccaggcggta tttccgctga tagataaacg aggtgagtac aatcgggatg
     5281 atgatggcga taaccattgc cagcgcaaat acctgccagt agctcggttg aatcgagaga
     5341 attcccggca ggccgcctac gccgatacca ttcgccataa cgccgttcag gccgcatagt
     5401 aatcctgcca gaccagaacc aatcatcgcg cacagcatcg ggaagcgata tttcaggttg
     5461 atgccgtaca ttgcaggctc agtgacccca agccaggcgg agatagcggc aggcacggag
     5521 atctcgcgtt cattgtgctt gcggctggaa atgataatgc ctatcacggc ggagccctga
     5581 gcgatattcg acagcgcaat cagcggccac actggcgtgc cacccatgct ttgaatcatc
     5641 tgcaaatcaa tagcaagggt ggtctggtgt acaccggtga tcaccagcgg ggcgtacagg
     5701 aagccaaaca atgctgcgcc aatcggagca aagctgccgg tcatcaggtg acgtaccgca
     5761 aaggcaacgc catcgccaat catgcgacca aacggaccaa tcagcgcatg ggcgaggaac
     5821 accgcgagga tcagcgaaca gacgggcacc accaccagat agaggtaatc cggcacgatg
     5881 cgtttaaggc gagtttcaat aacgcccagc gccagcccgg ctaacagtgc cgggatcacc
     5941 tgcgcctgat agcctacttt ggcgatgctg aacatgccaa agtcccacac ttccggcagc
     6001 tgctgcccga gcagataagc gttcatcagc tggggagaaa ccagtgtcac gccaagcacg
     6061 ataccaagga tcggcgtgcc gcccattttt ttcaccgctg accagcaaat accgaccggc
     6121 aggtagaaga agatcgcttc accgatcaac cacagaaaat cgtagatcgt ttgcagggaa
     6181 gggtacattt gcgccagcgt ctgaccgttg ctcatgggca aatcgccgat cacattgcga
     6241 aaaccgagga tcaaaccgcc gctaatcaac gcgggcagca gcgggaagaa gatctccgcg
     6301 aaatgagaga tcaactgctc atgccatttc atattctgcc gggcggcttt ttttacctgc
     6361 tctttatcaa cctgcgcctg tccggttgac gcaatcagtg cttgatagta atcacccaca
     6421 ttggtgccaa tcaccacctg aaattgcccg gcattggtga aacagccttt caccatgggg
     6481 agttgctcaa tttctttcgg tctggcattg gccggttggt tgaggacaaa gcgcaggcga
     6541 gtaatacagt ggctcaccgt cgcaatattg ccgcgcccgc cgaccagttc aatcaaccga
     6601 tcgatatccg tttggtttat tttgctcatc ataaagcccc atggcagatg acatttttgg
     6661 ttggctgcag aatatattgc gcggaaaaat ttaaaaacgg gaacgttccc gaaacacagc
     6721 gaagatcaca atttatcgtt caggaaacga tcaggacagg gtggcgggga tgatgatttg
     6781 ttgcggttcg ctgcgcccgg ttacctgtgc gatcaattgg caggccgcct ggcgtccagc
     6841 ttcggcgtaa ccaggatcta cggtgacgat ctccggatga aggaatttca ttaacggcgt
     6901 attaccgacg ctcgccagtt gcaaggtgtc gatgcgttgc tcttgcaggt atttacttgc
     6961 gccaagtgcc agcgtgtcgg ttgcgcacag taaggcggta gtttcaggcg taatcacttt
     7021 tgctacgttc tcatagcctt gcttcatagc aagccctggc agagcggcaa cgggatgcag
     7081 tttatgcgct ttgcagaacg ccaggtaggc ttcgtgacgt cgcttaccgg tcgtcacgtc
     7141 actgtgcggc acgccgagat aactgatatt acgatgcccc tggtcataga gccgttgcat
     7201 caggatcttg attgcccctt cgtcgtcata acagaccgaa gcaaagcctt ttgcgtcacg
     7261 cgccagcaga accagcgatg actgccagtg ggctaacatt tcttctgtta tgccggtaaa
     7321 accgaacagc actacgccgt cgatattacg ccgttttagg atccccaaat gttcggcaac
     7381 taattgcggg gaaaactgac tttccatcat gattgggtcg taaccttgtt catagaacgc
     7441 tggcagcatg gtttgaacgg cgagattttc tgacaacgaa tccaggcggg taacaatgat
     7501 ggcgaccact ttatcgcttt gcccacgcat agcgcgcgca gagcgggaag gggaaaatcc
     7561 atgctgattc atcactgctt ccacgcgctc gcgcgtgcgc tggctcacgc cgctttcgtt
     7621 attcagcacc cgggaaaccg tagatttccc cacgccgctt aagcgcgcga tgtctttgat
     7681 ggtcagccga ttttgcatcc tgttgtcccg taacgtgttg tttaattatt tgaggctaac
     7741 tttacccgtg cattcagcaa tgggtaaagt ctggtttatc gttggtttag ttgccagcag
     7801 gtattatatc gccataaatg ctacgaatat tattggattc tccttattat ttgcggcgct
     7861 tttttcactt accggaggtt atatggaacc tgatcccacg cctctccctc gacggagatt
     7921 aaaacttttt cggtaagccc gtcttttcac ggcgttaccg gatgcgtaag gccgtgacgt
     7981 tttaacgtcc ctgctcagct ttattacctt caggtaaggc ttcgccacgc ctgaagacat
     8041 ttctgtactg tttcagacag tgcggaggga ctccttatgt ttaaagaaat ttttacccgg
     8101 ctcattcgcc atttaccttc ccgtctggtt catcgtgatc cattgcctgg cgcgcagcag
     8161 acagtgaata cggtggtccc gccgtcctta agtgcgcatt gcctgaaaat ggcggtgatg
     8221 cccgaagaag aattgtggaa aacgttcgac acccatccgg aagggttaaa tcaggcggaa
     8281 gtggaatctg cccgcgaaca acatggtgaa aataaattac ccgcacaaca accgtcgccg
     8341 tggtgggtac atttatgggt ctgctatcgc aaccccttta atattttact caccattctc
     8401 ggcgctattt cttacgccac ggaagattta tttgccgcgg gcgttatcgc gctaatggtc
     8461 gccatttcta cgttgctgaa ctttattcag gaagcacgtt ccactaaagc ggcagatgcc
     8521 ctgaaagcga tggtcagcaa tactgcgacg gtgctgcgcg taattaacga caaaggcgaa
     8581 aatggctggc tggagatccc gatcgaccag ctggtgcccg gcgatattat aaaactggcg
     8641 gcgggggata tgatcccggc agatttacgt atcttgcagg cgcgggatct gttcgtcgct
     8701 caggcgtcgt taaccggtga gtctctgccc gtagaaaaag cagctaccac tcgccagccg
     8761 gagcacagca atccgctgga gtgcgacacc ctgtgtttta tgggcaccac cgtggtgagc
     8821 ggcacggcac aagcaatggt gattgctaca ggtgccaata cctggtttgg tcaactggcg
     8881 gggcgtgtta gtgagcagga aagcgagccg aatgcctttc agcaagggat cagccgcgtc
     8941 agtatgctgc tgattcgctt tatgctggtg atggctccgg tggtgctgtt aatcaatggt
     9001 tacaccaaag gcgactggtg ggaagcggca ctgtttgcgc tttcggtcgc ggtaggctta
     9061 acgccggaaa tgttgccgat gattgttacc tcgacgctgg cgcgcggcgc agtaaagctg
     9121 tcgaaacaga aagtgatcgt caaacatctg gatgctattc agaactttgg cgcaatggat
     9181 attctgtgca ctgataaaac tggcaccctg acacaggaca aaattgtgct ggagaatcat
     9241 accgatatct ccggtaaaac cagcgaacgc gtgctgcata gcgcgtggtt gaacagtcat
     9301 tatcagaccg gacttaaaaa cctgctcgat acagcggtgc tcgaaggtac ggatgaagag
     9361 tcagcgcgct cgctggccag tcgttggcaa aaaatcgatg agattccgtt tgatttcgag
     9421 cgtcgccgga tgtcggtggt agtggcagaa aataccgagc accatcagct ggtttgcaaa
     9481 ggtgcattgc aggaaatcct caatgtgtgt tcgcaggtgc gtcacaatgg cgagattgtg
     9541 ccgctcgatg acatcatgct gcgtaagatt aagcgggtta ctgatacgct gaatcgtcag
     9601 gggctgcgcg tggttgcggt ggcgacgaaa tacctgccag cgcgtgaagg agattaccag
     9661 cgggcggatg aatccgacct gatcctcgaa ggatatattg ctttccttga tccgccaaaa
     9721 gagacaactg ctccggcact gaaggcatta aaagcgagtg gaattaccgt aaaaatcctt
     9781 actggcgaca gtgagttagt tgcagcgaaa gtgtgccatg aagtggggct ggacgcggga
     9841 gaggtggtca ttggtagtga tattgaaacg ctatctgacg acgaactggc aaatctcgca
     9901 cagcgcacca cgctgtttgc tcgtctgacg ccgatgcata aagaacgcat tgtgacctta
     9961 ctgaagcgcg aagggcatgt ggttggcttt atgggcgatg gtattaatga tgcgcccgcc
    10021 ttacgcgctg cggatatcgg catttctgtg gacggcgcgg tagatattgc ccgtgaagcg
    10081 gctgatatca tcctgctgga aaaaagcctg atggtgctgg aagagggggt tattgaggga
    10141 cgtcgcactt tcgccaacat gctgaaatac atcaaaatga cggcgagctc taacttcggt
    10201 aatgtgttca gcgtgctggt ggcgagtgct ttcttgccct tcctgccgat gttgccgtta
    10261 cacttgctta ttcagaacct gctgtacgat gtgtcacagg tggcgatccc gtttgataac
    10321 gtcgacgacg agcaaattca aaagccgcag cgttggaatc cggcggatct ggggcgcttt
    10381 atgatcttct tcggaccgat cagctcgatc ttcgatattc tgacgttttg cctgatgtgg
    10441 tgggtattcc atgccaacac gccggaaacg caaacgctgt tccagtcggg atggtttgtg
    10501 gtgggcttac tgtcgcaaac gctgattgtg catatgatcc gtacccgccg tgtgccgttt
    10561 attcagagct gtgcatcgtg gccgttaatg atcatgaccg tgatcgtgat gattgtcggg
    10621 atcgcattgc cgttttcacc gctggccagt tatctgcagc tgcaggcgct gccgttaagc
    10681 tatttcccgt ggctggttgc gattctggca gggtatatga cgttaaccca gttggtgaaa
    10741 gggttctata gccgtcgtta cggctggcag taaagaataa actgggcacg atagccccga
    10801 ttcagattgc agacaaagtg agcgttgttc atgccggatg cggcgtaaat gccttatccg
    10861 gcctacaaat tcaatcaatt gcaggaatca cgtagatact gataagcgaa gcgcatcagg
    10921 caatgatacg tttgtcaccc agcctctgga tcacatcagt tcgggcactt ataaacctga
    10981 ccgataatct ggctatccgt cgggacaaaa ctggacaaca ttccctgcga cgggctgctg
    11041 atgccataaa tcacgttacc gcccattgca gccgcctggt tgcgcagatc gtttgctgcg
    11101 ccgcgcatag aaccgccctc ttctccgtgt tgcccggaaa gccagttgct ttgcttacct
    11161 gtcgcagtac caatcagctg gcactctgcg cctggctgct cgtccacaat gcgtacactc
    11221 tgaccggcag cactcaattc gttgctggag ctacaacccg ccatcagtag cgcggcaccg
    11281 acaatccctg ctaagtattt tacgtgcatg ttattcccca taatcaatga gctggacgcc
    11341 actcgcgtcg tccgtgtctg aatcttatac tagaaagatg accaaaagaa aaacccccag
    11401 acatttctgc ccgggggttt gtttatttct gcgaggtgca gggcaattac atcatgccgc
    11461 ccatgccacc catgccgccc ataccgccag cagcgcctaa gtcagctgca tcatttttcg
    11521 gcaggtcggt aaccatgcac tcggtggtga tcatcaggcc agccacagaa gccgcgtact
    11581 gcagagcaga acgggttact ttggttgggt ccaggatacc catgtcgatc atgttgccgt
    11641 attcttcggt tgctgcgttg taaccgtagt tgccgtcgcc gcctttaacg gtgttagcaa
    11701 caacagacgg ttcttcgccg cagttcagga cgatctgacg cagcggagct tccattgcac
    11761 gcagtgcaac tttgataccc acgttctggt cttcgttctg accacgcagg tcagccagtt
    11821 tagacgctac gcggatcagc gcaacaccac caccagcaac cacgccttct tctaccgcag
    11881 cacgggtcgc gtgcagggcg tcttcaacgc gtgctttttt ctctttcatt tcaacttcgg
    11941 tagcagcacc tactttgata actgcaacgc cgcctgccag tttcgctacg cgttcctgca
    12001 gtttttcacg gtcgtagtca gaagttgctt cttcaatctg ctgacggatc tgagcaacac
    12061 ggccctggat tgcagcttct tcacccacgc catcgatgat agtggtggtg tctttgttga
    12121 tcacaacacg tttagcctga cccaggtctt ccagggttgc tttttccagc tccataccga
    12181 tctcttcaga gattacggta ccgccagtca gggttgcgat atcctgcagc atagctttac
    12241 gacgatcgcc gaagcccgga gctttaaccg cagcaacttt cacgatgcca cgcatggtgt
    12301 taacaaccag agttgccagc gcttcgcctt caacatcttc agcgatgatc agcagcggtt
    12361 tgcctgcttt ggcaacagct tccagaaccg gcagcatttc gcggatgtta gagattttct
    12421 tgtcagccag caggatgaac gggctttcca gttctactgc gccagtttcc ggcttgttga
    12481 tgaagtaagg agacaggtag ccacggtcga actgcatacc ttcaaccacg tccagttcgt
    12541 cctgcagacc ggtaccgtct tcaacggtga taacgccttc tttaccgact ttgtccatcg
    12601 cttcagcgat cagtttacct acggtttcgt cggagttagc ggagatggta ccaacctgag
    12661 caatcgcttt agagtcagag cacggtacgg acagcgcttt cagttcttca actgcagcgg
    12721 taacggcttt gtcgatacca cgtttcaggt ccatcgggtt catgcccgca gcaacagctt
    12781 tcagaccctc agtgatgata gcctgagcca gtacagttgc agtggtggta ccgtcgcctg
    12841 cagcgtcgtt cgctttagag gcaacttctt tcaccatctg cgcacccata ttttcgaact
    12901 tgtcttccag ttcgatttca cgagcaacgg aaacaccatc tttggtgatg gtcggtgcac
    12961 cgaaagattt atccagaact acgttacggc ctttcggacc gagggtaact ttcactgcat
    13021 ctgccagtac gtttacgccg cgcagcattt tcacacgagc gtcgttaccg aattttacgt
    13081 ctttagctgc cattatcttt attccttaaa ttcgtatgtt cagtgtcgtg cgaggattac
    13141 gcttcaacaa ttgccagaat gtcgctttcg gacatgatca acacttcttc attgtcgatc
    13201 ttctcagatt tcacaccgta gccatcgttg aaaataacga tgtcgccaac tttcacatcc
    13261 agcggcttca cttcgccatt ttcaaggata cggccattgc cgacagccag cacttcgccg
    13321 cgggtggatt tagccgctgc agagccggtc agaacgatgc cgccagcaga tttagtttca
    13381 acttctttac gcttgacgat cacgcgatca tgcaatggac gaatattcat tgataactct
    13441 cctttgagaa agtccgtatc tgttatgggt gacgccggag cttacgtggt ttcccggctg
    13501 gtgaccagag aaatggggat gaggattcgc cccttcaagg gggaaaaaga aaaaaaattc
    13561 tgatcagcac aaaatcgggt gaaaaccctg attcacctca catttcatcg caatttcttc
    13621 atcgccgtca taagcgaatc tgattgtgct accatcgaaa atctacgcag ttgcccaaaa
    13681 tttgggcgca atcggatatc aacccatgag tggactcaaa caagaactgg ggctggccca
    13741 aggcattggc ctgctatcga cgtcattatt aggcactggc gtgtttgccg ttcctgcgtt
    13801 agctgcgctg gtagcgggca ataacagcct gtgggcgtgg cccgttttga ttatcttagt
    13861 gttcccgatt gcgattgtgt ttgcgattct gggtcgccac tatcccagcg caggcggtgt
    13921 cgcgcacttc gtcggtatgg cgtttggttc gcgacttgag cgagtcaccg gctggttgtt
    13981 tttatcggtc attcccgtgg gtttgcctgc cgcgctacaa attgccgccg ggttcggcca
    14041 ggcaatgttt ggctggcata gctggcaact gttgttggca gaactcggta cgctggcgct
    14101 ggtgtggtat atcggtactc gcggtgccag ttccagtgct aatctacaaa ccgttattgc
    14161 cggacttatc gtcgcgctga ttgtcgctat ctggtgggcg ggcgatatca aacctgcgaa
    14221 tatccccttc ccggcacctg gtaatatcga acttaccggg ttatttgctg cgttatcagt
    14281 gatgttctgg tgttttgtcg gtctggaagc atttgcccat cttgcctcgg aatttaaaaa
    14341 tccagagcgt gattttcctc gcgcattgat gattggtctg ctgctggcag gattagtcta
    14401 ctggggctgt acggtagtcg tcttacactt cgacgcctat ggtgaaaaaa tggcggcggc
    14461 agcatcgctt ccaaaaattg tagtgcagtt gttcggtgta ggagcgttat ggattgcctg
    14521 cgtgattggc tatctggcct gctttgccag tctcaacatt tatatacaga gcttcgcccg
    14581 cctggtctgg tcgcaggcgc aacataatcc tgaccactac ctggcacgcc tctcttctcg
    14641 ccatatcccg aataatgccc tcaatgcggt gctcggctgc tgtgtggtga gcacgttggt
    14701 gattcatgct ttagagatca atctggacgc tcttattatt tatgccaatg gcatctttat
    14761 tatgatttat ctgttatgca tgctggcagg ctgtaaatta ttgcaaggac gttatcgact
    14821 actggcggtg gttggcgggc tgttatgcgt tctgttactg gcaatggtcg gctggaaaag
    14881 tctctatgcg ctgatcatgc tggcggggtt atggctgttg ctgccaaaac gaaaaacgcc
    14941 ggaaaatggc ataaccacat aatccggcgt ttcgacatta atcctggcga tcgtctttat
    15001 gatcaaggcg gtcgcgctca tcatcctttc gctggtactc accatcaaaa gtattaccgc
    15061 caccggtccc ggcgctaaaa ccgccgcctg gcatacgaga aaagcgcaaa tgcggcatca
    15121 acttcactgt cagatgcttt tgcaccggcg gtaataaaag cagaagaccg aggaagtcgg
    15181 tgaaaaagcc cggtaataaa agcagcaaac cggcaatgat cagcgaaaca cttttaatca
    15241 tctccgccgc tgggttttca cccgccgcca ttttttgctg catcagcacg aaattcttaa
    15301 agccctggtt acgtaccagt gacatcccga taactgacgt gaatataacc agcacgaggg
    15361 tcagcaatac ccccaataca tgggcaacct gaataaagat tgaaatctca atatagacat
    15421 aaaggaaaat ggcaataaaa ggtaaccagc gcaaaggttt ctcctgtaat agcagccggt
    15481 aaaacccggc tacctgaatg ggttgcgaat cgcgttcagc ttatattgtg gtcattagca
    15541 aaatttcaag atgtttgcgc aactattttt ggtagtaatc ccaaagcggt gatctatttc
    15601 acaaattaat aattaagggg taaaaaccga cacttaaagt gatccagatt acggtagaaa
    15661 tcctcaagca gcatatgatc tcgggtattc ggtcgatgca ggggataatc gtcggtcgaa
    15721 aaacattcga aaccacatat attctgtgtg tttaaagcaa atcattggca gcttgaaaaa
    15781 gaaggttcac atgtcaaaca acattcgtat cgaagaagat ctgttgggta ccagggaagt
    15841 tccagctgat gcctactatg gtgttcacac tctgagagcg attgaaaact tctatatcag
    15901 caacaacaaa atcagtgata ttcctgaatt tgttcgcggt atggtaatgg ttaaaaaagc
    15961 cgcagctatg gcaaacaaag agctgcaaac cattcctaaa agtgtagcga atgccatcat
    16021 tgccgcatgt gatgaagtcc tgaacaacgg aaaatgcatg gatcagttcc cggtagacgt
    16081 ctaccagggc ggcgcaggta cttccgtaaa catgaacacc aacgaagtgc tggccaatat
    16141 cggtctggaa ctgatgggtc accagaaagg tgaatatcag tacctgaacc cgaacgacca
    16201 tgttaacaaa tgtcagtcca ctaacgacgc ctacccgacc ggtttccgta tcgcagttta
    16261 ctcttccctg attaagctgg tagatgcgat taaccaactg cgtgaaggct ttgaacgtaa
    16321 agctgtcgaa ttccaggaca tcctgaaaat gggtcgtacc cagctgcagg acgcagtacc
    16381 gatgaccctc ggtcaggaat tccgcgcttt cagcatcctg ctgaaagaag aagtgaaaaa
    16441 tatccaacgt accgctgaac tgctgctgga agttaacctt ggcgcaacag caatcggtac
    16501 tggtctgaac acgccgaaag agtactctcc gctggcagtg aaaaaactgg ctgaagtcac
    16561 tggcttccca tgcgtaccgg ctgaagacct gatcgaagcg acctctgact gcggcgctta
    16621 tgttatggtt cacggcgcgc tgaaacgcct ggctgtgaag atgtccaaaa tctgtaacga
    16681 cctgcgcttg ctctcttctg gtccacgtgc cggcctgaac gagatcaacc tgccggaact
    16741 gcaggcgggc tcttccatca tgccagctaa agtaaacccg gttgttccgg aagtggttaa
    16801 ccaggtatgc ttcaaagtca tcggtaacga caccactgtt accatggcag cagaagcagg
    16861 tcagctgcag ttgaacgtta tggagccggt cattggccag gccatgttcg aatccgttca
    16921 cattctgacc aacgcttgct acaacctgct ggaaaaatgc attaacggca tcactgctaa
    16981 caaagaagtg tgcgaaggtt acgtttacaa ctctatcggt atcgttactt acctgaaccc
    17041 gttcatcggt caccacaacg gtgacatcgt gggtaaaatc tgtgccgaaa ccggtaagag
    17101 tgtacgtgaa gtcgttctgg aacgcggtct gttgactgaa gcggaacttg acgatatttt
    17161 ctccgtacag aatctgatgc acccggctta caaagcaaaa cgctatactg atgaaagcga
    17221 acagtaatcg tacagggtag tacaaataaa gaaggcacgt cagatgacgt gccttttttc
    17281 ttgtgagcag taacttaaaa ataacaacct aatatcaact tgttaaaaaa caaggaaggc
    17341 taatatgcta gttgtagaac tcatcatagt tttgctggcg atcttcttgg gcgccagatt
    17401 ggggggaata ggtattggtt ttgcaggcgg attgggggtg ctggttcttg ccgctattgg
    17461 cgttaaaccc ggtaacatcc cgttcgatgt catttccatt atcatggcgg ttatcgccgc
    17521 tatttctgcc atgcaggttg ctggcggtct ggactatctg gttcatcaga cagaaaagct
    17581 gctgcgccgt aacccgaaat acatcacgat cctcgcaccg atcgtgacct atttcctgac
    17641 tatctttgct ggtactggca acatatctct ggcgacactg ccagttatcg ctgaagttgc
    17701 gaaggaacaa ggcgtcaaac cttgccgtcc gctgtctact gcagtggtat ccgcgcagat
    17761 tgcgatcacc gcatcgccaa tctcagcggc agtggtttac atgtcttccg tgatggaagg
    17821 tcatggcatc agctacctcc atctgctctc cgtggtcatc ccgtccaccc tgctggcggt
    17881 tctggtgatg tcctttctgg tcactatgct gttcaactcc aaactctctg acgatccgat
    17941 ttatcgcaag cgtctggaag agggcctggt tgaactgcgc ggtgaaaagc agattgaaat
    18001 caaatccggt gcaaaaacgt ccgtctggct gttcctgctg ggcgtagttg gcgtggttat
    18061 ctatgcaatc atcaacagcc caagcatggg tctggttgaa aaaccgctga tgaacaccac
    18121 caacgcaatc ctgatcatca tgctcagcgt tgcaactctg accaccgtta tctgtaaagt
    18181 cgataccgac aacatcctca actccagcac cttcaaagca ggtatgagcg cctgtatttg
    18241 tatcctgggt gttgcgtggc tgggcgatac tttcgtttcc aacaacatcg actggatcaa
    18301 agataccgct ggtgaagtga ttcagggtca tccgtggctg ctggccgtca tcttcttctt
    18361 tgcttctgct ctgctgtact ctcaggctgc aaccgcaaaa gcactgatgc cgatggccct
    18421 ggcactgaac gtttcaccgc tgaccgctgt tgcttctttc gctgcggtgt ctggtctgtt
    18481 cattctgccg acctacccga cgctggttgc tgcggtacag atggatgaca cgggtacaac
    18541 ccgtatcggt aaattcgtct tcaaccatcc gttcttcatc ccgggtactc tgggtgttgc
    18601 cctggccgtt tgcttcggct tcgtgctggg tagcttcatg ctgtaatgac caatcgcggg
    18661 gcgttcacgc cccgctttct ttcccgccga ctaacatcct tcccccgtcc gttgtatagt
    18721 gacctctctc ttgcggttcc atctgttctt gcgaggtgtt tatgcttgat gaagaaagtt
    18781 cgaataccgc gtctgtcgtg gtgctatgta ccgcaccgga tgaagcgaca gcccaggatt
    18841 tagccgccaa agtgctggcg gaaaaactgg cggcctgcgc gaccttgatc cccggcgcta
    18901 cctctctcta ttactgggaa ggtaagctgg agcaagaata cgaagtgcag atgattttaa
    18961 aaactaccgt atctcaccag caggcactgc tggaatgcct gaagtctcat catccatatc
    19021 aaaccccgga acttctggtt ttacctgtta cacacggaga cacagattac ctctcatggc
    19081 tcaacgcatc tttacgctga tcctgctact ttgcagcact tccgtttttg ccggattatt
    19141 cgacgcgccg ggacgttcac attttgtccc cgcggatcaa gcctttgctt ttgattttca
    19201 gcaaaaccaa catgacctga atctgacctg gcagatcaaa gacggttact acctctaccg
    19261 taaacagatc cgcattacgc cggaacacgc gaaaattgcc gacgtgcagc tgccgcaagg
    19321 cgtctggcat gaagatgagt tttacggcaa aagcgagatt taccgcgatc ggctgacgct
    19381 tcccgtaacc atcaaccagg cgagtgcggg agcgacgtta actgtcacct accagggctg
    19441 tgctgatgcc ggtttctgtt atccgccaga aaccaaaacc gttccgttaa gcgaagtggt
    19501 agccaacaac gcagcgtcac agcctgtgtc tgttccgcag caagagcagc acaccgcgca
    19561 attgcccttt tccgcgctct gggcgttgtt gatcggtatt ggtatcgcct ttacgccatg
    19621 cgtgctgcca atgtacccac tgatttctgg catcgtgctg ggtggtaaac agcggctctc
    19681 cactgccaga gcattgttgc tgacctttat ttatgtgcag gggatggcac tgacttacac
    19741 ggcgctgggt ctggtagttg ccgccgcagg gttacagttc caggcggcgc tacagcaccc
    19801 atacgtgctc attggcctcg ccatcgtctt taccttgctg gcgatgtcaa tgtttggctt
    19861 gtttactcta caactcccct cttcgctgca aacacgtctc acgttgatga gcaatcgcca
    19921 acagggcggc tcacctggcg gtgtgtttgt tatgggggcg attgccggac tgatctgttc
    19981 accatgcacc accgcaccgc ttagcgcgat tctgctgtat atcgcccaaa gcgggaacat
    20041 gtggctgggc ggcggcacgc tttatctcta tgcgttgggc atgggcctgc cgctgatgct
    20101 aattaccgtc tttggtaacc gcttgctgcc gaaaagcggc ccgtggatgg aacaagtcaa
    20161 aaccgcgttt ggttttgtga tcctcgcact gccggtcttc ctgctggagc gagtgattgg
    20221 tgatgtatgg ggattacgct tgtggtcggc gctgggtgtc gcattctttg gctgggcctt
    20281 tatcaccagc ctacaggcta aacgcggctg gatgcgcgtg gtgcaaataa tcctgctggc
    20341 agcggcattg gttagcgtgc gcccacttca ggattgggca tttggtgcga cgcataccgc
    20401 gcaaactcag acgcatctca actttacaca aatcaaaacg gtagatgagt taaatcaggc
    20461 gctcgttgaa gtcaaaggca aaccggtgat gttagatctt tatgccgact ggtgcgtcgc
    20521 ctgtaaagag tttgagaaat acaccttcag cgacccgcag gtgcaaaaag cgttagcaga
    20581 cacggtctta cttcaggcca acgtcacggc caacgacgca caagatgtgg cactgttaaa
    20641 gcatcttaat gtccttggcc taccgacaat tctctttttt gacggacaag gccaggagca
    20701 tccacaagca cgcgtcacgg gctttatgga tgctgaaacc ttcagcgcac atttgcgcga
    20761 tcgccaaccg tgaacgacac ttgcagtggg aaagacggag gaaaatagcg tgcaacgtga
    20821 agatgtactg ggagaagccc tgaaattatt agaattacaa gggattgcca acaccacgct
    20881 ggagatggtt gctgaacgtg tggattatcc actggacgag ctacgccgct tctggccaga
    20941 caaagaggca atcctctacg atgcgctgcg ttatcttagc caacagatag atgtctggcg
    21001 tcgtcagctg atgctggacg aaacgcaaac cgccgaacaa aagctgctgg cacgttatca
    21061 ggcgttatcg gagtgcgtta aaaacaaccg ctatccgggc tgtctattta tcgctgcctg
    21121 tacgttttat cccgatcctg gccaccctat tcatcaactg gccgatcagc aaaaaagcgc
    21181 ggcctacgat ttcacccacg aactgttaac cacgctggaa gttgacgatc cggcgatggt
    21241 agcaaagcag atggaactgg tgctggaagg ctgtttaagc cgaatgctgg tgaatcgtag
    21301 ccaggcggat gtcgacactg cacatcggct ggcggaagat atcctgcgct tcgcccgctg
    21361 ccgccagggt ggtgcattga cctgacagaa acacagaaaa gaagcgattt gccgcaatct
    21421 taagcagttg aatcgctttt actgaaatta ggttgacgag atgtgcagat tacggtttaa
    21481 tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc
    21541 ttggttcgat tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt
    21601 ttttcgtttc agaacaccat gatttaagtg ttcttcc
//
LOCUS       DAZGOB010000075        21561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGOB010000075 DAZGOB010000000
VERSION     DAZGOB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.075"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..429
                     /gene="decR"
                     /locus_tag="ACHLIF_003061"
     CDS             <1..429
                     /gene="decR"
                     /locus_tag="ACHLIF_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO9607037.1"
                     /translation="ALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIGKVAL
                     LDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGEYDYLMRVQVA
                     DMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            459..2231
                     /locus_tag="ACHLIF_003062"
     CDS             459..2231
                     /locus_tag="ACHLIF_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO9607038.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHRALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            2224..4005
                     /locus_tag="ACHLIF_003063"
     CDS             2224..4005
                     /locus_tag="ACHLIF_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO9607039.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            4186..4524
                     /gene="glnK"
                     /locus_tag="ACHLIF_003064"
     CDS             4186..4524
                     /gene="glnK"
                     /locus_tag="ACHLIF_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO9607040.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            4554..5840
                     /gene="amtB"
                     /locus_tag="ACHLIF_003065"
     CDS             4554..5840
                     /gene="amtB"
                     /locus_tag="ACHLIF_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO9607041.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(5889..6749)
                     /gene="tesB"
                     /locus_tag="ACHLIF_003066"
     CDS             complement(5889..6749)
                     /gene="tesB"
                     /locus_tag="ACHLIF_003066"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO9607042.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            6967..7539
                     /locus_tag="ACHLIF_003067"
     CDS             6967..7539
                     /locus_tag="ACHLIF_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO9607043.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(7570..7881)
                     /locus_tag="ACHLIF_003068"
     CDS             complement(7570..7881)
                     /locus_tag="ACHLIF_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO9607044.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            8043..8139
                     /gene="ffs"
                     /locus_tag="ACHLIF_003069"
     ncRNA           8043..8139
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHLIF_003069"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8259..8612
                     /locus_tag="ACHLIF_003070"
     CDS             8259..8612
                     /locus_tag="ACHLIF_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO9607045.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(8654..10204)
                     /gene="pdeB"
                     /locus_tag="ACHLIF_003071"
     CDS             complement(8654..10204)
                     /gene="pdeB"
                     /locus_tag="ACHLIF_003071"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO9607046.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGQLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(10368..10838)
                     /locus_tag="ACHLIF_003072"
     CDS             complement(10368..10838)
                     /locus_tag="ACHLIF_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO9607047.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(10954..11505)
                     /gene="maa"
                     /locus_tag="ACHLIF_003073"
     CDS             complement(10954..11505)
                     /gene="maa"
                     /locus_tag="ACHLIF_003073"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO9607048.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPVRIIKKL"
     gene            complement(11677..11895)
                     /locus_tag="ACHLIF_003074"
     CDS             complement(11677..11895)
                     /locus_tag="ACHLIF_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO9607049.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(11921..12295)
                     /gene="tomB"
                     /locus_tag="ACHLIF_003075"
     CDS             complement(11921..12295)
                     /gene="tomB"
                     /locus_tag="ACHLIF_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO9607050.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(12841..15990)
                     /gene="acrB"
                     /locus_tag="ACHLIF_003076"
     CDS             complement(12841..15990)
                     /gene="acrB"
                     /locus_tag="ACHLIF_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO9607051.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDREKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(16013..17206)
                     /gene="acrA"
                     /locus_tag="ACHLIF_003077"
     CDS             complement(16013..17206)
                     /gene="acrA"
                     /locus_tag="ACHLIF_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO9607052.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            17348..17995
                     /gene="acrR"
                     /locus_tag="ACHLIF_003078"
     CDS             17348..17995
                     /gene="acrR"
                     /locus_tag="ACHLIF_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO9607053.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQVKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            18123..21485
                     /gene="mscK"
                     /locus_tag="ACHLIF_003079"
     CDS             18123..21485
                     /gene="mscK"
                     /locus_tag="ACHLIF_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO9607054.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
BASE COUNT         5162 a         5362 c         5697 g         5340 t
ORIGIN      
        1 gccttactgc agcaggattg caccctctct ttgcaggcac tggctgaagc cgttaatctg
       61 acaaccaccc cttgctggaa gcgtctgaaa cggctggagg acgacggtat ccttatcggc
      121 aaagtcgccc tgctggatcc ggaaaaaata ggcctcggcc tgaccgcttt tgtgctgata
      181 aaaacgcaac atcacagcag cgaatggtat tgccgctttg tcacggtggt taccgaaatg
      241 ccagaagtgc tggggttctg gcgcatggct ggtgaatacg attatctgat gcgcgtccag
      301 gttgccgaca tgaaacgcta cgacgagttt tataagcgtc tggtaaacag cgtgccgggg
      361 ctgtcggacg tcacttccag cttcgcgatg gaacagatta aatacaccac ttctttaccc
      421 atcgaataaa tatccagaat caggtcagga cacaacgcgt gcgattattt gctcaattaa
      481 gctggtattt ccgtcgggaa tggcgtcgct atctcggggc tgtcgccttg cttgtcatta
      541 tcgcgatgct gcaactggtt ccgccaaaag tggttggtat tgttgtcgat ggcgtgacag
      601 aacaacactt tactaccggg ctgatcctga tgtggatcgc caccatggtg ctgattgccg
      661 ttgtggttta tctcctgcgt tatgtctggc gcgtattgct gtttggcgcg tcttaccaac
      721 tggctgttga actgcgtgaa gattattacc gtcagctaag ccggcagcac ccggagtttt
      781 acctgcgtca tcgcacgggt gatctcatgg ctcgtgcgac caatgacgtc gatcgcgtcg
      841 tgtttgccgc cggagaaggg gtgctgacgc tggtggattc actggtaatg ggctgcgctg
      901 tgttgattat gatgtctacg caaattagct ggcagttgac cttatttgcc ctgttaccga
      961 tgccagtgat ggcgatcatg attaagcgca acggcgatgc cttgcatgaa cgctttaagc
     1021 tggcacaggc ggcgttttcc agtcttaatg accgcaccca ggaaagcctc accagtatcc
     1081 gcatgatcaa agcctttggt ctggaagatc gccagtcggc gttatttgcc gcggatgccg
     1141 aagataccgg caaaaaaaac atgcgggtgg cgcgtattga tgctcgtttc gacccgacaa
     1201 tctatatcgc gattggtatg gcgaacttgc tggcgattgg cggcggtagt tggatggtgg
     1261 tgcagggcag tttaacgctg ggccagctca ccagttttat gatgtattta ggtctgatga
     1321 tttggccaat gctggcgctg gcatggatgt ttaacattgt ggaacgtggt agtgctgcgt
     1381 acagccgtat tcgtgcgatg ctggcggaag cgccggtggt gaacgatggt agcgaacccg
     1441 tgccggaagg gcgtggcgaa cttgatgtaa atattcacca gttcacgtat ccgcagactg
     1501 accatcgtgc gctggaaaac gtcaatttcg ccctgaaacc cggtcagatg ctgggtatct
     1561 gcgggccgac tggttccggc aaaagtaccc tgttgtcgct cattcagcgt catttcgacg
     1621 tcagcgaggg ggatattcgc tttcatgata ttcctctgac gaagttacaa ctcgatagct
     1681 ggcgtagccg cctggcggta gttagccaga caccgttcct tttttccgac accgtagcga
     1741 acaacatcgc gttgggttgc ccgaatgcca cccagcaaga gattgagcat gtcgcgcggt
     1801 tagccagcgt acatgacgat attttgcgtt taccgcaagg ttacgataca gaggtgggcg
     1861 agcgcggcgt gatgctttcc ggcgggcaaa aacagcgtat ctctattgct cgtgcgttat
     1921 tagtcaacgc ggaaatcctc atccttgatg atgcgctttc ggcggtggac ggacgcacag
     1981 agcaccagat cctgcataac ctgcgtcagt ggggacaggg tagaacggta atcatcagtg
     2041 cccatcgcct ttcggcactg acggaagcca gtgaaattat tgtgatgcag cacggacata
     2101 tcgcccagcg tggcaatcat gatgagctgg cgcaacaaag cggctggtat cgcgatatgt
     2161 atcgctatca acaactggag gcggcgctcg acgacgctcc ggaaattcgc gaggaggccg
     2221 tcgatgcgta gttttagcca actgtggccg actctcaagc gtctgttagc gtacggttcg
     2281 ccgtggcgta aaccgctggg gattgcggtc ctgatgatgt gggttgcggc agcggcagaa
     2341 gtcagtgggc cgctgcttat cagctatttt atcgacaata tggtagcgaa aaataacctg
     2401 ccgttgaaaa tggttgcagg gctggctgcg gcgtatgttg ggctacaact gtttgccgcc
     2461 gggctacatt acgcgcagtc gctgctgttt aatcgggcgg cagtaggcgt agtgcaacag
     2521 ttgcgtaccg acgtgatgga tgccgcattg cgccagccat taagcgagtt tgatacccaa
     2581 cccgtcgggc aggtgatttc ccgcgtcact aatgacactg aagtgatccg cgatctctac
     2641 gttaccgtag tggcaactgt cctgcgtagc gccgcactgg tgggcgcgat gctggtggcg
     2701 atgttcagcc tcgactggcg aatggcgctg gtggcgataa tgattttccc ggtggtgctg
     2761 gtggtaatgg tgatatacca gcgttacagc acgccgattg tccgtcgtgt gcgcgcctat
     2821 ctagcggata tcaacgacgg ctttaacgaa atcatcaatg gcatgagcgt tatccagcag
     2881 ttccgtcagc aggcgcgatt tggcgaacgt atgggggagg ccagtcgttc acactatatg
     2941 gccaggatgc aaaccctgcg cctcgacggt tttctgctgc gtccgctgct gagtctgttt
     3001 tcatcgctca ttctttgtgg cttgttgatg ctgtttggct tctccgctag cggcaccatt
     3061 gaagtgggcg tgctgtatgc gtttatcagc tatcttgggc gacttaacga accattaatc
     3121 gaactgacca cgcaacaggc gatgctgcaa caggctgttg ttgctggtga gcgcgtgttt
     3181 gaactgatgg acggaccgcg ccagcaatat ggcaatgatg atcgcccgtt acagagtggc
     3241 accatcgaag tcgataacgt gtcatttgct tatcgcgatg acaatctggt gctaaagaac
     3301 attaatctct ctgtgccttc gcgtaacttt gtggcactgg tcgggcatac cggcagtggc
     3361 aaaagcaccc tcgccagttt attgatgggc tattacccgc taacggaagg tgagattcgc
     3421 cttgatggtc gtccattaag ttcgctaagt cacagcgcgc tgcgccaggg cgtggcaatg
     3481 gtgcagcaag atccggtggt gctggcggat accttcctcg ccaacgtgac gctggggcgt
     3541 gatatctccg aagaacgcgt ctggcaggcg ctggaaaccg tgcaactggc ggagctggcg
     3601 cgcagcatga gtgatggtat ttatacgccg ctgggcgagc aggggaataa tctctccgtc
     3661 gggcaaaagc aactgctggc actggcacgc gtgttggtgg agacgccgca aatcctgatc
     3721 cttgatgagg caaccgccag cattgactcc ggtactgaac aggcgattca acatgctctg
     3781 gcggctgtgc gtgaacatac cacgctggtg gtgattgctc accgcttatc gaccattgtt
     3841 gatgccgaca ccattctggt gcttcatcgt gggcaagccg tggagcaggg cactcaccag
     3901 caactgctgg cggcccaggg acgctactgg cagatgtatc aactgcaact tgcgggcgaa
     3961 gagctggcag ccagcgtgcg tgaagaagaa tcattgagcg cctgaatagc gcaatatttc
     4021 atcgttggtg caaaaatgta acgcactgtg cactgtcata gtgcgttttc attttcaatc
     4081 ttcttaactt cctgctctct ttctcgtttt tcatttctgg cacaccgctt gcaatacctt
     4141 cttcgtgtag cagaaccatt accgaattct gaccggaggg gatctatgaa gctggtgacc
     4201 gtgataatca aaccattcaa gctggaagac gttcgtgaag cgttatcttc cattggtatt
     4261 cagggcctga ccgtcaccga agtgaaaggt ttcgggcgtc agaaagggca tgccgagctg
     4321 taccgggggg cggaatacag cgtcaacttc ctgccaaaag taaaaattga tgtggcgatt
     4381 gctgatgacc aactcgatga agtgatcgat atcgtcagta aggcggctta caccggaaaa
     4441 attggcgacg gcaaaatctt cgtcgctgaa ttgcaacgcg tcattcgtat tcgtaccggc
     4501 gaagccgacg aagcggcgct gtaatctctg gcacacagca acaggaacga aaaatgaaga
     4561 tagcgacgat aaaaactggg cttgcttcac tggcgatgct gccgggactg gtaatggctg
     4621 cacctgcggt ggccgataaa gccgacaatg cgtttatgat gatttgtact gcgctggtgc
     4681 tgtttatgac tattccgggg attgccctgt tttacggtgg gttgattcgc ggcaaaaacg
     4741 tgctgtcgat gctgacgcag gtgacggtga cgtttgcact ggtctgtatt ctctgggtgg
     4801 tttacggtta ctcgctggcg tttggtgagg gcaacaactt cttcggcaac attaactggt
     4861 tgatgctaaa aaacatcgaa ctgacggcgg tgatgggcag catttatcag tatatccacg
     4921 tggcgtttca gggctcgttt gcctgcatta ccgtcggctt gatcgttggg gcgctggcgg
     4981 aacgaatccg cttctcagct gtgttgattt tcgtggtggt atggctgacg ctctcttaca
     5041 ttccgattgc gcatatggtg tggggcggtg gtttgctggc ttctcacggt gcgctggatt
     5101 tcgcgggtgg cacggtggtg catattaacg ccgcaatcgc cggtctggtg ggcgcgtatc
     5161 tgataggcaa acgcgtgggc ttcggcaaag aggcgtttaa accgcacaac ctgccaatgg
     5221 tcttcaccgg tactgccatt ctctatattg gttggtttgg ctttaacgcc gggtcagcgg
     5281 gcacggcgaa tgaaatcgcg gcactggcat ttgtgaatac tgtggtcgca acggcggcgg
     5341 caattcttgg ctggatcttc ggtgaatggg cgctgcgtgg taagccttct ctgctgggag
     5401 cgtgttctgg cgcgattgcc ggtctggtcg gcgtgacgcc agcgtgtggc tacattgggg
     5461 ttggcggcgc gttgattatc ggcgtggtag ctggtctggc gggcttgtgg ggcgttacta
     5521 tgctcaaacg cttgctgcgg gtggatgatc cctgcgatgt cttcggtgtg cacggcgttt
     5581 gtggcattgt cggctgtatc atgactggga tttttgccgc cagttcgctg ggtggcgtgg
     5641 gcttcgctga aggtgtgacg atgggccatc agttgctggt acagctggaa agcatcgcca
     5701 ttacgatcgt ctggtccggt gtggtggcat ttattggcta caaactggcg gatctgacgg
     5761 ttggtctgcg tgtaccggaa gagcaggagc gagaagggct ggatgtcaac agtcacggcg
     5821 agaatgccta taacgcgtaa caagcactgc aaaaaatagc cggacggttt tacctccggc
     5881 tatttttttt aattgtgatt acgcatcacc ccttcctgaa cggtcgaggc aaccagcacg
     5941 ccgtcctggg tataaaactc accgcgcaca aagccgcgtg cgctggacgc cgaggtgctc
     6001 tccacgctat acagcagcca ttcattcaaa ttaaacgggc gatggaacca catggaatgg
     6061 tcaatggtgg caatctgaat ccccggttcg agaaaaccga tgccgtgcgg ctgtagagct
     6121 accggcagga agttaagatc agaagcgtaa ccgagcagat actgatgaac gcgcaggtca
     6181 tccggcacgc taccatttgc gcggatccac acctgacgat gtggttctgc gacgtgacct
     6241 ttcagtgggt tatgaaactc caccggacgg acttccagcg gacgatcgca gatgaattta
     6301 tctttaagca ctggcggcag caggtgcgcc agcgattggg cgatttgcgt ttccgaaggc
     6361 aggccatcag gcgctggcgc ggacggcatt gttttttgat gttcgaaacc cgcttctggt
     6421 gcctggaaag aggccgtcat ataaaaaatc ggtttgccgt tttgaatagc agcaacccgg
     6481 cgggcgctga agctgttacc gtcacgcagc gtttcgacat cataaataat cggcttctta
     6541 ctatcgccag ggcgaagaaa gtagctgtga aacgaatgta ccagccgctc ttcagggacg
     6601 gtctcttttg cagcatacaa ggcctgaccc acgacctggc cgccaaacac ctggcgtaaa
     6661 cctaaatctt cactctggcc gcgaaagagt ccttcctcaa ttttttccag atttaacaat
     6721 gtcagtaaat tttttagcgc ctgactcata taactctcca gtaacaaagc tgccgcagca
     6781 agccaaagtg agttgagtat aacgcaaatt tgctactggt ccgatgggtg caatggtctg
     6841 aattacgggc taattacagg cagaaatgcg tgatgtgtgc cacacttgtt gacgttacta
     6901 ttttgttaac cactcttccg gcgaggaaag ttagcccgct ggtgcattga taataaggag
     6961 aaatgaatga aactcgtgca catggccagt ggtttagcgg ttgcgattgc gttggcggct
     7021 tgcgcagata aaagcgcgga tattcagacg ccagccccgg ctgcaaatac atctatttca
     7081 gcaacacaac aacctgctat ccagcaaccg aatgtctccg gtaccgtctg gatccggcag
     7141 aaagtcgcac tgccgcctga tgctgtgctg accgtgacac tttctgacgc gtcgttagcc
     7201 gatgcaccgt caaaagtgct ggcgcagaaa gcggtgcgta ccgaaggtaa acagtcacca
     7261 ttcagctttg ttctgccatt taatccggca gatgttcagc cgaacgcgcg tattctgttg
     7321 agtgcggcga ttaccgtgaa tgacaaactg gtatttatta ccgataccgt tcagccggtg
     7381 atcaacaagg gcggaactaa agccgacctg acattggtgc cggtgcagca aaccgccgtg
     7441 ccggttcagg ccagcggtgg tgcaacgact accgtacctt cgacttcacc aactcaggtg
     7501 aatccgtctt cggcagttcc agctcctacg caatattaag cgacgttaac cctctccgcc
     7561 cggagagggt tagtagttcc agcgataacg ctgcaagtcg atttgcccgc ttcccgatac
     7621 catcacacct tctgccagta atgcctgtcg ctgacgctgt aaatccggtc cggttagcga
     7681 aattgtgccg tggcgattaa ccacccggtg ccagggtaag gtgctgcctt cagggagacg
     7741 ctttaacaca ccgcccacct ggcgcgcggc gcggggcgat cccgccagtt tcgccacatc
     7801 accgtaagtg gtgacatagc cttcgggaat agcggcgacg atttgccaga cgcgttgggg
     7861 aaatgaatct tctttttcca tcttttcttc ctgaggtaat ttttcagcat aatctggaaa
     7921 aacgcccgag tgaagtcgca ttgcgcaaga aaccagcatc tggcacgcga tgggttgcaa
     7981 ttagccgggg cagcagtgat aatgcgcctg cgcgttggtt ctcaacgctc tcaatggggg
     8041 cctgttggtt ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc
     8101 aaggcagatg acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc
     8161 caccgtttcg tcaaaaaaaa ccaacatggc taaactttaa ccacaactga cgtcgcaaga
     8221 attgtctggc tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg
     8281 tggttgccgt tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc
     8341 cattgtttaa agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg
     8401 atggcaaagt gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct
     8461 ttagctggat tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt
     8521 cggacccacg gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct
     8581 atggcggatt cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg
     8641 tcagcccaaa aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg
     8701 ccttgctgta gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt
     8761 gtttactggt ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga
     8821 tgatatgcgg cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca
     8881 gaatgtcgac atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat
     8941 caagataaat ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg
     9001 gatcggcaaa ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgatagt
     9061 gattgatcat ttcacgcagc aattgcggga ttttttccga ggtgagcacg gtggattcaa
     9121 gattgatcga aatatgctgc tgtggatgct gacgcagcca gtcgcccata tcttcaaaga
     9181 cgcttcttat aatcagtagc gtcaatggct cagaaaggcc cgtttgctgt gccagcggaa
     9241 taaaactatc tggtgacaac caactaccgt ctgtctgcgg ccagcgcgcc agtgcctcag
     9301 caccgacaat tttgccattg gctaaggaga caatcggctg atagtgcacg caaatatcac
     9361 gattttcgat agcatcctgc agccgatgat gcggtgactg gatacggcgc aaaatacgca
     9421 gcacaaacat cgctgccagc tggccaatca ccaacccgag cggtaaccaa ataaagactt
     9481 gccgatgcca gcctttctgc aacattttcg ttgaagccca ggtgatgatc gaaatattca
     9541 tctccgggaa gggcaggata tcgtaaatga ttccattatt ttcgatatgc tcaccgggtg
     9601 ttttttgtag cctggtaata attccctgag caatttcatc gctgctggtt atgacaacgt
     9661 tatgggcatt gccaataatg gcggcatcaa tttgccatga gctataggga atgacatcaa
     9721 taaaggaagc ggggtcgatc atgacgacat aatgtgccgt tcccatggcg accatgtaac
     9781 ggataatgcc taaatcgtta tgcgatgtta accagacacg ataaccatct ttcgaaattt
     9841 tacctggctc ggggaaggta tcgggcggac tttcatgctc cagagacgaa cactggggaa
     9901 cgttgttatc gatataaacc acttcctgaa tatagcgata actgtaagat acccgacgca
     9961 tttccatgag atgggcttcg ctacaggcag cgccttgcca tctttccagc tcttgcagcg
    10021 catctttccc ttgtgtcgcc accttattgg cccgaatagc gacgcgggag gaataggtat
    10081 ccagctcttc aataaacgat gtttctacct gctgatgggc cagccagatg cttaagccga
    10141 caggcagcaa tactgaaaga atcagtactc ccgaaataag gccgaccaga tgtcgtgttc
    10201 tcaccagcat atccttatta acacagtgta gggaaagtat atctgattgt ggaaaaaggc
    10261 tggcaggaga taaggaggta gagctggtat ttcatgaagt gatgggtgga aacgatcatt
    10321 gtcatttgac caggcgcaga gctttacgcc tggtatgtaa ccctaactta agatgttgat
    10381 tcggcgcgag cgagggtaaa aatatcgtaa aaagacagtt cacctttacg gacgatcatt
    10441 ttttgcagtt gttccttatg aacatccgcg acacgcggag agttcaagat ggcttcaacc
    10501 agcgcggcag gtacaaagcg cgtgttgtac cattcgccgt tatagcaaac gcggaaatcc
    10561 agcacgtcga tatcttcata gcggtagcgt gggtagacat cgaaaaagaa gaaaccatta
    10621 agcaggataa ataatacaac cagtagccag acagagcctg acagcgtttc ggactgcaac
    10681 atcaccgcca gggtggcaaa aaaagcaacg tacataccga taaacagccc cggatgttta
    10741 cggataaaac tgatactaaa gcggggtttg ttgtcgcgtt tttcgcgggt attcagagac
    10801 tcaatcgttt cggtcagcag gcgttgtatt tcggtcatcg tttgccttcg tggtatctgc
    10861 aactttccag ggaacaccct attttaacgt gggaatagta gaggggaagt aacagatcta
    10921 ccacaatttt gcataacagt tgcgaaaaac cgattacaat tttttaatta ttctgactgg
    10981 attaccgccc acgacaacgt tgtccgggac atcttttgtg acaactgcac ctgaggctac
    11041 cacgacgtta tcaccaatgg tcacaccagg gttaatgacc gcgcgtccgc caatccagac
    11101 gttattaccg atggtgacgg gtttccccag ttcagcaccg ctattacgtg ctacagggtc
    11161 gatgggatgt gttgccgtgt agatatgaac gcctggtgcc aacatacagt tatcaccgat
    11221 gcgaataggg cagacatcaa gcatcacgca atcgaagttg gcgaaaaaat tattaccgag
    11281 aaaaatgtta tagccatagt cacagcgaaa cgttggctca atataagcct ctgtcacctg
    11341 accgaataga tcagcgagaa tttgctggcg taatgtgtgc tcttccgcca gggaatgatt
    11401 gtatcggtga ataagctgac gagcgcgcag gcgatcgcga gataacgtct catctgccga
    11461 gcgatacaac tcaccagcaa tcatcttttc tttttctgtg ctcattactg acctccggtt
    11521 gctgaaaatg cggcaacaca gtaagacgaa aagtcagtga agagaacgtt cctattacat
    11581 ctgtgtgata aagatcacat agggtatgtc ttcatggtga aaaagtataa aattcttaat
    11641 aaacagccgg ttatagctcc gaaagcgaat tatttattag cgaataaatt tccatactga
    11701 ggaagggatc ttgtcgtata gtttattcat ggtcaattcg gcgaggcggt gatctgcggc
    11761 tgagtaaaat accgccagtt cattatctga taattcgtat ttatttttct cgataacacg
    11821 ctccagcgtg tcaattgtct ggcaacgacg taaacgcatt aaataatcgg ttttcgttaa
    11881 aggtttttcg gacatacttc tacccatggt tgtaataatt ctaacaagat aaactcgcag
    11941 gattctcttt cgtcgcattg acaaaacaac ggaatagtcg attacctgac ttccgccatt
    12001 tctgaagatc ctgcatatta ataccataac tgctgaacaa cataaaggtg tcatccagat
    12061 attcgtcgat ctgctcaatg agcttattgt cttcattata cttaattttg taattaagtg
    12121 cgaaggtcgc aatatgctca atcagttcat tcaactggag gttgatcgcc gaggttgggt
    12181 cgttaaccca gccatgattg ctttcttcaa ggtttgcaag acagtcatga tacagggttt
    12241 cacagagaaa cttaagctgt gcgatatcat gtcttttggg tgagtattca tccataacgc
    12301 gtccccttct tagtggttga actaacggac acctttcggg atggaaaaaa cttactgacc
    12361 tggacttgcc ctctcgtttg ttagcttaac tataagccac tctttgcagg ttttcatcgc
    12421 attattacga aaaattacaa ttaggaagga atcgggacag gactcacttt ttcatattag
    12481 cactaacccg cagcaggtaa aagcagtttg ctcgaactgt tcataacatt tcaggattat
    12541 cgccagatga aattggtgac actaatacca ggattgctct gaatatgacg taataaccga
    12601 ggaatgaata aagaattacc gcaaataatt aagaatagcc tctaaatgat tatggattat
    12661 taaatctatt agcgctactt aatataaatt aactaacaat cagtattttt tatgaatttt
    12721 atccgtggtt aatactggtt ttcgtatgag atcctgagtt ggtggttcaa ttactcctta
    12781 atgttcgtag gttatgcata aaaaaggccg cttgcgcggc cttagtgatt acacgttgta
    12841 tcaatgatga tcgacagtat ggctgtgctc gatatcttca ttcttgcggc taaagcggcg
    12901 gcgaaccacc acaaagaata ccggaacgaa gaagattgcc agtaccgttg cggtcaccat
    12961 cccgcccatt acaccggtac ctactgcgtt ctgcgcgccg gaaccagcac cagtactgat
    13021 aaccagcggc ataacgccga ggataaacgc cagcgaggtc atcaggatcg gacgtaaacg
    13081 catccgcact gcatcaagcg tcgcttcaat cagaccttta ccttctttat ccatcaagtc
    13141 tttggcgaat tcgacgataa gtatcgcgtt cttcgccgac aacccaatgg ttgtgagcag
    13201 gcctacctgg aagtaaacgt cattggtcag gccacggaag gtggcagcca gcaacgcacc
    13261 gataaccccc agcggaacga ccagcataac ggagaacgga atcgaccagc tctcgtacag
    13321 cgccgccaga cacaggaata cgacaatcaa cgaaatcgcg tacagtgaag gtgcctggtt
    13381 gccggagagg cgttcctgat aggacatccc cgtccagtca tagccaacac cggtaggcag
    13441 tttgctcgcc agttgttcca tcagctccat tgcttcaccg gtacttttac ccggtgccgc
    13501 ctggcctaag atttccatgg atggcaggcc attgtaacgt tccagacgcg gcgaaccgta
    13561 ctcccaacga gaagaggaga atgccgagaa cggcaccatc tgaccatcag cagcacgaac
    13621 ataccagtcg ccgatatcat ccggcagcat acggtatttc gcttctgaca tgacgtaaac
    13681 tttcttcaca cgaccgcggt cgataaagtc gttcacatag ctaccgcccc atgcagcgcc
    13741 cagagtggtg ttaatgtcat tgatagaaac acccagcgcc tgcgcttttt cccggtcgat
    13801 atcaatctta aactgcgggg tatcttccag accgtttgga cgtacgctgg tcaacatatc
    13861 agggtgcttc gctgcttctg caagcaactg gttacgcgcc tgagtcagtt tttcgtgacc
    13921 aaggccagcc tggtcaatca gctcaaagtc aaagccggtt gcagtaccca gttccacgat
    13981 tgcgggcagg ttaaaggcga aaaccatcgc atctttgatt tgcgagaaag cgcgtgttgc
    14041 acgcatggta atcgcttcaa ctttgttttc ttcgcccgga cgatcggccc agtccttcaa
    14101 ggaaacgaac gcaataccgg tattctgacc acgtcccgca aagccgaagc cgttaacggc
    14161 gaacaccgac tcaacgttgt tcttttcttt ggtcagatag taatgcgtta cctcattgag
    14221 cactttctgc gtacgttcct gcgttgcacc tgctggcagc tgaaccatgg tcataaatac
    14281 gccctggtcc tcatctggca agaaggagct tggcagacgc acgaacagat aggccatgcc
    14341 gaccacgatg atcagataca gcaccaggta acgccccgta ctgcgcagaa taccgcctac
    14401 gctgtcggtg tagtggtgcg tgctcttctc gaacatgcgg ttaaaccagc cgaagaagcc
    14461 ttttttacct tccccgtgat cgcctttggc aatcggtttc agcatggtgg cacaaagagc
    14521 cggagtcagg atcaacgcta ccagtaccga cagcgccatt gctgaaacaa tggtaataga
    14581 gaactgacga tagatagcac cagtagaacc gccaaagaag gccatcggta cgaataccgc
    14641 cgacagtacc atcgcgatac cgaccagagc gccctgaatc tgccccatcg acttacgggt
    14701 agcttctttt ggcggcaaac cttcttccgc cataacacgc tccacgtttt ctaccacaac
    14761 gatagcgtca tccaccaaca agccgatggc gagcaccatc ccgaacattg ttagcgtgtt
    14821 tatcgagaag ccaaaggcgg caaggacggc aaaggtcccg agcaatacca ccggtacggc
    14881 aatggtcgga atcaacgtcg cgcggaagtt ctgcaggaac agatacataa ccaggaacac
    14941 gaggatgatc gcttcgacca gcgttttaac cacttcgtga atagagattt tcacgaacgg
    15001 cgtggtgtcg tacgggtaaa caattttcag acccgacggg aagaacggtt ccatcttcgc
    15061 cagttcagca cggattgccg cagcggtatc cagcgcgttt gcaccggtcg ccagcttgat
    15121 ccccagaccg gaagccggtt ggccgttaaa ctctgcgatg atgtcgtagt tctcaccacc
    15181 cagctcaatc ttcgccacat cacgcagcag tacgcgggaa ccatcctgat tcactttcag
    15241 caggattttg ccgaactctt cagtagaggt cagacgcgtc tgagcaataa tagaggcgtt
    15301 aagctgttgg cctttcaccg gcggcgtacc accgagctga cccgccgcaa cctgggcgtt
    15361 ctgcgctttg atggcggtaa tgacatcaac cggcgttagc tggaatttgt tcagctcatt
    15421 cgggttcatc cagatacgca tcgcgtactg tgaaccgaac aactgaacat cacccacgcc
    15481 cgacgtacgg ctgatggcat ctttcatatt cgccgctacg tagtcagaga tatcctcctg
    15541 cgtcatggtg ccatcggtgt tgataacgcc gacaaccatc aggaagctgc tggatgattt
    15601 ctcaacgctc accccttgct gctgaacttc ttgcggcagc aacggcatcg ccagctgcag
    15661 tttgttctgc acctgaacct gcgcgatatc cgcatcagta ccagactcaa aggtcagggt
    15721 gatctgcacg gtacccgtgg agtcactgtt agaggacatg tacatcaggt tatcgatacc
    15781 gttcatattc tgttcgataa cctgtgtcac cgtgtcctgc actgttttcg catcagcgcc
    15841 ggggtaggag gcggagatcg ttactgccgg cggtgcaatc gtaggatatt gcgccaccgg
    15901 cagtttgagg atcgccagcc cccctgccaa catgatgata atggcgatca cccacgcaaa
    15961 aatcgggcga tcgataaaga aattaggcat gtcttaacgg ctcctgttta agttaagact
    16021 tggactgttc aggctgagca ccgcttgcgg cttgctggtt attatcagcg gtaacttctt
    16081 gtgcttttac ctggacacca ggacgcactt tctgcagccc acttattact acgcgatcgc
    16141 ctgctttcag accttctgtc accagccact tatcgccaat agcctggctt gcaacgatcg
    16201 gacgggtttc cactttgtca tccgcgccaa ctaccagtac ggtggcatca ccacgcggcg
    16261 tacgggttac gccctgttgc gggactaaaa tagcgtttgg attaagccct tcttccagac
    16321 gtgcgcgcac gaacataccc ggcagcagag tgtgatccgg gttcgggaag atagcgcgta
    16381 gggtgataga cccagtggtc tgatcaacgg taacgtcaga gaattccagc gtaccgtcct
    16441 gcgggaactt aatgccgtca ctggtgatca gcgacacttt ggctttgcca ttctcttgtt
    16501 tcagcgtgcc attcgccagt tcctgtttca ggcgcaggaa gtcgttgctg gactgggtca
    16561 catcaacgta gatcggatca agttgctgca cggttgccag cgcagtcgcc tgaccgttct
    16621 gtaccaatgc gccttccgtc acgttcgact taccaatgcg accgctaatc ggagaggtga
    16681 ctttggtgta agccagattg atccgcgcag tttcaacggc agctttcgcc gcagttaccg
    16741 cagcattcgc ctgttgcgca tcagccagag cctgatcgta ctcttgctta ctgatgtact
    16801 gagtaccgag cagtttctga taacgattca ccgtcaattg cgcgatattg gctgcagcct
    16861 gggctttcgc cagatcacct ttcgcactgt cgtatgtcgc ctgataggtc gcaggatcaa
    16921 tctgatagag agagacacca gcttcgatgt cgctaccttc tttgaaatta cgcttcagga
    16981 taatcccgct aacttgagga cgaacttctg cgatccggta ggcactggtg cgacccggaa
    17041 gctcggttgt gatctgcaga ggttcagttt tgactgttac tacgccaacg gcgggcatct
    17101 gctggccacc ttgttgggcc tgtttgtcgt cacatcctgt tagggctaag ctgcctgaga
    17161 gcatcagaac gaccgccaga ggcgtaaacc ctctgttttt gttcatatgt aaacctcgag
    17221 tgtccgattt caaattggtc aatggtcaaa agttaataaa cccattgctg cgtttatatt
    17281 atcgtcgtgc tatggtacat acattcacaa atgtatgtaa atctaacgcc tgtaaattca
    17341 cgaacatatg gcacgaaaaa ccaaacaaga agcgcaagaa acgcgccaac acatcctcga
    17401 tgtggctcta cgtcttttct cacagcaggg ggtatcatcc acctcgctgg gtgagattgc
    17461 aaaagcagct ggcgttacgc gcggtgcaat ctactggcat tttaaagaca agtcggattt
    17521 gttcagtgag atctgggaac tgtcagaatc caatattggt gaactagagc ttgagtatca
    17581 ggtaaaattc cctggcgatc cactctcagt attaagagag atattaattc atgttcttga
    17641 atccacggtg acagaagaac ggcgtcgatt attgatggag attatattcc acaaatgcga
    17701 atttgtcgga gaaatggctg ttgtgcaaca ggcacaacgt aatctctgtc tggaaagtta
    17761 tgaccgtata gaacaaacgt taaaacattg tattgaagcg aaaatgttgc ctgcggattt
    17821 aatgacgcgt cgcgcagcaa ttattatgcg cggctatatt tccggcctga tggaaaactg
    17881 gctctttgcc ccgcaatctt ttgatcttaa aaaagaagcc cgcgattacg ttgccatctt
    17941 actggagatg tatctcctgt gccccacgct tcgtaatcct gccactaacg aataaccctg
    18001 aatctgactc caggattttt cctggacatt ttcgtcgttg ctattctggt tcactgcgtc
    18061 gtgatattct tgcggtttga ctttttcagg tcgttcttca ggttcagaaa ccttcattca
    18121 tcatgactat gttccagtat tacaaacgat cacggcattt tgttttttca gcatttattg
    18181 cttttgtttt tgtcttgtta tgccagaaca cggcgtttgc gcgggcgtca tcgaatggtg
    18241 atctgccgac aaaagcggac ctgcaggcgc aacttgactc actaaataaa caaaaagatc
    18301 tttctgctca ggacaaactg gtgcagcagg atctgacaga tacattagcc accctcgata
    18361 aaatcgatcg cataaaagaa gagacagttc agctacgaca aaaagtcgct gaagcgccgg
    18421 aaaaaatgcg ccaggcgacc gcggcgttaa cagcacttag cgatgtcgat aacgacgaag
    18481 aaacgcgcaa aattctgagt acgctttcgt tgcgccagct ggaaactcgc gttgcccagg
    18541 cgctggacga tttgcaaaac gcacaaaacg atctggcgtc ttataacagc cagctggttt
    18601 cgttacagac gcagcccgaa cgcgtgcaaa atgcgatgta taacgcttcg cagcagctgc
    18661 aacaaattcg cagtcgtctg gatgggactg atgtcggcga gactgcctta cgtcccagcc
    18721 agaaagtgtt aatgcaggct cagcaggcgt tgctgaatgc ggagattgac cagcagcgta
    18781 aaagcctgga agggaacacc gtcttgcagg ataccttgca aaagcaacgt gattacgtga
    18841 cggcgaacag cgcccgtctg gagcaccagt tgcagctgtt gcaagaagcg gtaaacagca
    18901 agcgcctgac tttaactgaa aaaacggcgc aggaagccgt ctccccggat gaagccgcgc
    18961 gtattcaggc taatccgctg gtgaaacagg aactggaaat taaccagcag ttaagtcagc
    19021 gtctgattac cgcgactgaa aacggtaatc agttgatgca gcaaaacatt aaagtcaaaa
    19081 actggctgga gcgggcgctg caatcggaac gcaatattaa agagcagatt gccgtcctga
    19141 agggcagcct gctgttgtct cgtatccttt accagcaaca acaaacgctg ccctcggcgg
    19201 atgaactgga aaacatgacc aaccgcatcg cggatttgcg tctcgaacag tttgaagtta
    19261 accagcagcg tgatgcactc ttccagagcg atgcgttcgt caacaaactg gaagaaggtc
    19321 acaccaacga agtcaacagc gaagttcacg atgcgttatt gcaagtggtt gatatgcgtc
    19381 gcgaattgct ggatcaactc aacaaacagt tgggtaacca gctgatgatg gccattaacc
    19441 tgcaaatcaa ccagcagcag ttaatgagcg tatcgaaaaa cctgaaatcc atcctgactc
    19501 agcaaatctt ttgggtgaac agtaaccgtc caatggactg ggactggatc aaagcgttcc
    19561 cgcaaagcct gaaagatgaa tttaagtcga tgaaaatcac ggtgaactgg gaaaaagcct
    19621 ggcctgccgt ttttatcgct ttcctcgccg gtttgccgct gctgttgatt gccgggctga
    19681 tccactggcg tctgggctgg ctgaaagcgt atcaacagaa actggcttcc gctgtgggtt
    19741 ccctgcgtaa cgatagccag ctcaacacac caaaagcgat ccttatcgac ctgatccgcg
    19801 cgctgccggt gtgcctgatt attctcgcgg ttggcctgat tctgttgacc atgcagctca
    19861 acatcagcga actgctatgg tcgttcagta aaaaactggc gattttctgg ctggtgtttg
    19921 gcctgtgctg gaaggtactg gagaaaaatg gcgttgccgt gcgtcacttc ggcatgccgg
    19981 agcagcagac cagccactgg cgtcggcaaa ttgtccgcat cagtctcgcg ttgctgccta
    20041 tccatttctg gtctgtggtg gcagaacttt ccccgctgca tctgatggat gatgtgctgg
    20101 ggcaggcgat gattttcttc aacctgctgc tgattgcctt cctggtatgg ccaatgtgcc
    20161 gcgaaagctg gcgtgataaa gagtcgcaca ccatgcgact ggtcaccatt accgtgctgt
    20221 cgattatccc gattgcattg atggtgctga ctgccacggg ttatttctac accacgctgc
    20281 gcctctccgg gcgttggatt gaaaccgttt atctggtgat catctggaac ttgctctacc
    20341 agacggtatt gcgcggctta agcgtagcgg cgcggcgtat cgcctggcgt cgtgcgttgg
    20401 cgcgtcggca gaatctggtg aaagagggcg cagaaggtgc cgaaccgccg gaagaaccca
    20461 ccattgcact ggaacaggtt aaccagcaga cgctgcgtat taccatgttg ctgatgtttg
    20521 cgctgttcgg tgtcatgttc tgggcaattt ggtccgattt gatcaccgtg ttcagctatc
    20581 tcgacagcat cacgctctgg cattacaacg gcactgaagc tggcgctgcg gtggtgaaaa
    20641 acgtcaccat gggcagtctg ttgtttgcga ttatcgcctc aatggtggcc tgggcgttga
    20701 ttcgcaacct gcctggttta ctggaagtgc tggtgctctc gcgactgaat atgcgtcagg
    20761 gcgcgtcgta tgccattact accatcctta actacatcat tattgccgtt ggtgcgatga
    20821 cggtgttcgg atcgctgggc gtctcttggg ataaactcca gtggctggcc gcagcattat
    20881 ccgtaggtct tggttttggt ttacaagaaa ttttcggtaa cttcgtctcc ggtttgatca
    20941 ttctgttcga acgtccggtg cgtattggcg atacggtaac cattggtagc ttctcgggga
    21001 cggtaagtaa gatccgtatt cgtgcgacaa cgattaccga tttcgatcgc aaagaagtga
    21061 tcatcccgaa caaagcgttt gttaccgagc gtctgatcaa ctggtcgttg actgacacta
    21121 ctacgcgtct ggtgatccgt ctcggcgtgg cctatggctc cgatctggaa aaagtgcgta
    21181 aagtgttact gaaggcggcg actgagcacc caagggtgat gcacgaacca atgccggaag
    21241 tcttctttac ggcgtttggt gccagcacgt tggatcatga gctgcgtctg tatgtgcgtg
    21301 aactgcgtga ccgtagtcgt actgtcgatg agctgaaccg tactatcgat cagctgtgcc
    21361 gtgaaaacga catcaacatt gcctttaacc agcttgaagt gcatctgcac aacgagaagg
    21421 gcgatgaggt gacggaagtc aaacgcgact acaaaggcga tgacccgacg ccagcggtag
    21481 ggtaataacc aaagggggcg acatttagtt gccccgagat tactgacaaa gtgcgcgttg
    21541 ttcatgccgg atgcggcgtg a
//
LOCUS       DAZGOB010000076        21489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGOB010000076 DAZGOB010000000
VERSION     DAZGOB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.076"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..329
                     /locus_tag="ACHLIF_003080"
     CDS             <1..329
                     /locus_tag="ACHLIF_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9607055.1"
                     /translation="KITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKW
                     SLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQ
                     PVSLVG"
     gene            complement(372..1523)
                     /gene="ydiO"
                     /locus_tag="ACHLIF_003081"
     CDS             complement(372..1523)
                     /gene="ydiO"
                     /locus_tag="ACHLIF_003081"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO9607056.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1537..3131)
                     /gene="ydiF"
                     /locus_tag="ACHLIF_003082"
                     /pseudo
     CDS             complement(1537..3131)
                     /gene="ydiF"
                     /locus_tag="ACHLIF_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            complement(3275..4033)
                     /gene="aroD"
                     /locus_tag="ACHLIF_003083"
     CDS             complement(3275..4033)
                     /gene="aroD"
                     /locus_tag="ACHLIF_003083"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO9607057.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4064..4930)
                     /gene="ydiB"
                     /locus_tag="ACHLIF_003084"
     CDS             complement(4064..4930)
                     /gene="ydiB"
                     /locus_tag="ACHLIF_003084"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO9607058.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4942..6207)
                     /gene="ydiN"
                     /locus_tag="ACHLIF_003085"
     CDS             complement(4942..6207)
                     /gene="ydiN"
                     /locus_tag="ACHLIF_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9607059.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6434..7648)
                     /gene="ydiM"
                     /locus_tag="ACHLIF_003086"
     CDS             complement(6434..7648)
                     /gene="ydiM"
                     /locus_tag="ACHLIF_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9607060.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVDSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7748..8104)
                     /gene="ydiL"
                     /locus_tag="ACHLIF_003087"
     CDS             complement(7748..8104)
                     /gene="ydiL"
                     /locus_tag="ACHLIF_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO9607061.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8240..8347)
                     /gene="rprA"
                     /locus_tag="ACHLIF_003088"
     ncRNA           complement(8240..8347)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHLIF_003088"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8533..9645)
                     /gene="ydiK"
                     /locus_tag="ACHLIF_003089"
     CDS             complement(8533..9645)
                     /gene="ydiK"
                     /locus_tag="ACHLIF_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO9607062.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10034..13090
                     /gene="ydiJ"
                     /locus_tag="ACHLIF_003090"
     CDS             10034..13090
                     /gene="ydiJ"
                     /locus_tag="ACHLIF_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO9607063.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13087..13497
                     /gene="menI"
                     /locus_tag="ACHLIF_003091"
     CDS             13087..13497
                     /gene="menI"
                     /locus_tag="ACHLIF_003091"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO9607064.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13597..13785
                     /gene="ydiH"
                     /locus_tag="ACHLIF_003092"
     CDS             13597..13785
                     /gene="ydiH"
                     /locus_tag="ACHLIF_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO9607065.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14333..14701
                     /gene="sufA"
                     /locus_tag="ACHLIF_003093"
     CDS             14333..14701
                     /gene="sufA"
                     /locus_tag="ACHLIF_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO9607066.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14710..16197
                     /gene="sufB"
                     /locus_tag="ACHLIF_003094"
     CDS             14710..16197
                     /gene="sufB"
                     /locus_tag="ACHLIF_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO9607067.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16207..16953
                     /gene="sufC"
                     /locus_tag="ACHLIF_003095"
     CDS             16207..16953
                     /gene="sufC"
                     /locus_tag="ACHLIF_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO9607068.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYRGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16928..18199
                     /gene="sufD"
                     /locus_tag="ACHLIF_003096"
     CDS             16928..18199
                     /gene="sufD"
                     /locus_tag="ACHLIF_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO9607069.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18196..19416
                     /gene="sufS"
                     /locus_tag="ACHLIF_003097"
     CDS             18196..19416
                     /gene="sufS"
                     /locus_tag="ACHLIF_003097"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO9607070.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19429..19845
                     /gene="sufE"
                     /locus_tag="ACHLIF_003098"
     CDS             19429..19845
                     /gene="sufE"
                     /locus_tag="ACHLIF_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO9607071.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19994..20998
                     /gene="ldtE"
                     /locus_tag="ACHLIF_003099"
     CDS             19994..20998
                     /gene="ldtE"
                     /locus_tag="ACHLIF_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO9607072.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21061..21297)
                     /gene="lpp"
                     /locus_tag="ACHLIF_003100"
     CDS             complement(21061..21297)
                     /gene="lpp"
                     /locus_tag="ACHLIF_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO9607073.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5589 a         5231 c         5434 g         5235 t
ORIGIN      
        1 aaaaaatcac tttagaacag ttatcgaaaa aatttcgtgc cagcgtcagt tatatttgcc
       61 atgaatttac caaagagtat cgtatttccc ctattaacta tgttattcaa cggcgtatga
      121 cggaagcaaa atggtcactc actaatactg aattatcaca ggcagagatc tcctggcgtg
      181 tgggttatga aaatgtcgat cactttgcca aactgttttt gcgccatgtc ggttgttcgc
      241 ccagcgatta ccgtagacaa tttaaaaact gttttgcgga acaagaaatc ctatctgaat
      301 ttcctcaacc ggtaagtctt gttggataaa tctggcggcg cagtttgtta agaagctgcg
      361 ccgcctgcag attatttgtt ctgatagtct ttcaggatct gccgacctgc tacgtaaatc
      421 ataatttcgt ctgtaccgcc gccgatacgt tcacaacgga catcacgcca gaagcgggag
      481 acgcgcgctt catcggtata gcccagaccg cccatgattt gaatcgcatc atcaatgact
      541 tccattgcgg tacgtgcgca atacagtttt gccagcgccg cgctggtgcg cagtgactga
      601 tgctgatcgg cttgccatgc cactttcagc accatgttgc gcatgttgtc gatcttaatc
      661 gccatcagcg ccagtttttc ctggatcatc tggttatgac caatgggctt accaaaagcg
      721 atacgttggt tggcatagcg ggcggcatct tcaaaggcgc attcggcaaa gccggtgctg
      781 cgcgcggcgt tgatcaggcg ctccatctca aagttgtaca tcacattgag gaaacccatt
      841 ccttcttcgc ccaccatgtc gctctcttca acttccacat tgtcgagata gacttcgcag
      901 gtgctgagca tatgccagcc gattttatgc agcgggttaa tcttaatgcc gggcttactg
      961 gagtcgaccc accacagggt gaaggctttt ttgggatctt tcggttgcgg atcgcgcgcc
     1021 agcaccagca tatacgggta ctctttcgca ccggtaataa aggttttttg tccgttgatg
     1081 tagaccttgc cgtttttacg cgtgtaagtg gtagtggcac tgttgttatc tgagccagca
     1141 cctggttccg tcaacgccag ggcataggcg ggatcacccg tttccagggt gctctctgcc
     1201 gttttacgta gctgctctgc agaaccgaaa cggcgcatac tgtgaataca ttgaccgttg
     1261 gtaattaaaa atgccggagc accgcatttt gacacttcca tcagcgccag catttgggtg
     1321 acgtaatccg cagggatacc accaaattct tccggcacgc caagcatgga aataccgtta
     1381 tccgccagcg cccgcataaa ctcacgcgga tatgtcccgt tttgatcgca ggtgcggaaa
     1441 tactcttccg gaaagttagt cgtaatcagt tcgcgaatac tggccagcag cagttcttgt
     1501 tcttcagtta aagaaaaatc catcattata ctcctgttaa tgagccgctt caggcaggac
     1561 aaaacccatc gccgcatcga taaataatct ttcgtccatc agtttgagtt ctggcgaaat
     1621 cactggggtg aagtccattt tgtcgagaat atctttttgt aaatcgacgc caggggcgat
     1681 ttcgattaaa tgcaggccgt cttctttcag cgtgaataca gcacgttcgg tgatgtaacg
     1741 aacatccagt ccccgttcga gcgcgatttt tccgctaaaa gtaatttccg gtagttcccg
     1801 aataaattta tttacccgtc cttcctggac gatatgcaat ttgccatcgg caatttctgt
     1861 ttttaaactg cccgcagtta acgtgccgca gaaaataatt ttcttcgagg tggcactgat
     1921 atcaataaat ccaccggtac ccatgatttt gccattgaat ttatgcacgc cgacgttacc
     1981 gtgctggtcg acttcagcaa aactcaaata acaaacatcc agaccaccac cgtgataaaa
     2041 atcaaactgg gacgtcatat ccagaatggc gcgggtattc acgttcgcgc caaaggcgat
     2101 accctgtgaa gtaattccgc caatcggacc tgtttctacc gtcagaataa agtcatcagc
     2161 acaaccttct tcccgggcga ccaggccaat accgtcagca ataccgacgc cgacattccc
     2221 caccgcgcct ttacgcattt cgaataatgc acgccgcgca actaatttac gttggtttag
     2281 gggcaggcta agtttgtgct gtcatcaagg gtgaagtcac cagaaataaa acggttaacc
     2341 ggcgcaccgc catacagttg gctttgatcc ggatcgacca ccacaatatc caccagataa
     2401 cccggaatac ggacagattt aggatgcagc gtggctttct taaccatttt ctgcacctgc
     2461 atcatcacaa taccgccgtt attgtgcacc gcctgggcaa taaccaatgc gtcgagatac
     2521 atcacctcat cttcaaaagt ggcgtagcct tcactgtcgc aggtggtagc gcgaatgaag
     2581 gcgatatctg gcgcaatcgc tttgtaatag agatattctt tgttatcaaa ctcgaccagt
     2641 ttaatcaggt cttctttcgt gacttcattc agtttgccgc cttgctggcg tggatcgaca
     2701 aatgtcccga tgccaatatc actaataata ccaggctggt gggctgcggc ggcgcgtaag
     2761 gtttgtgtaa gtacaccttg tggatagtta taagcaataa ttttattttg ttctgcgagt
     2821 tcagaaatac gcggcgattg tccccagtga ccacataatg cccatttcac cagaccttct
     2881 tgcgccagag gactaatacc acggtcggcg cgatcgccaa gccctgtcgg actaataatc
     2941 gataaattac gtggcgtttg agtctgttta tatttatcag caagagctgt aattaacgtg
     3001 gtggcttcca gaataccgcc gccagcgcct aacacacaaa gtgttgcttc gtcgggaata
     3061 taattcaccg cttcctgtgc cgacaggacc ggcactcgtc cattaatacg aggtggtttt
     3121 acaggtttca ttgttaattc ctgattcaga tgtgattttc cctacgccag atggagaatt
     3181 agcgcacaga gactcacgaa atatttttat taaatacggt taaatatatt ttttaagttc
     3241 ggcggggagg gtgttcccgc cgaaatatta ttgcttatgc ctggtgtaaa atagttaata
     3301 ccgtgcgtaa atcatttacc gagatttgcc ctggtgcaga ggcttttttt accgcaccaa
     3361 aagttgccgc cgagccaaat acttcaccag ccagacgaga aattacgcca gtttttgcca
     3421 tcgacatcgt gatgattgga cgatcggcat actgctcctg catctccagg gtcgcggtaa
     3481 gcaacgtcag cacatcgctg gtactttgcg gcatcagcgc aatcttagga atatcggcgt
     3541 cgaaggactg cattttgcgc agacgggcaa tgatttcttc ggcttccggc gttttatgga
     3601 agtcatggtt ggacatgaca actttcacat catgcgcgtg ggcgtaggcg acggtttctt
     3661 taacctgatc atcgccggta aataactcca gatcgatcat atcaaccagg ccgctgtcga
     3721 tggctgcacg attgagagca atataagcct cggtggaaat cgcctgctcg ccgccttctt
     3781 tggcactgcg gaaggtaaac agcagcggtt tttctggcat ggtttcacgg agaatttttg
     3841 ccgccgccat gacagactcc acattggaga ggtcggcaaa gtggtccaca cgccattcca
     3901 gaatatcaaa gtccgcttca cgataggcga gagcttcgga tttcacgcgg gcgatatctt
     3961 tcgccatcag cgagacgatg attttaggtg cgcccgtacc aatgacgaga tcttttacag
     4021 ttacggtttt cattttttac cctttctgca cgcggtcagc ctgtcaggca ccgaacccca
     4081 tgacctgttt aacatattcc agagggaaat ctttgccagt ccataacgtg aactgttcag
     4141 ccccttgcca caacaacatg ccgtatccat caatcgtttt gcaaccagct tgttgcgcct
     4201 gctgcaataa cttcgtcata tgcgggttat acacgcattc agtgaccagc agtcccggat
     4261 gtaacagact gatatcatta actaatgatt cattctcaag gggtttcata cccacttttg
     4321 tgccattggt taaaatgtcg gcggaagcca gggcttcagc aaaggcttgc tgatcggcga
     4381 gatcggtaac cgtgacgaca caatcggtgt tttcgttaac ccgctgcgcg aaggcgaggg
     4441 ctttatcgaa gaactcatcc cgacggttaa agagtttaat ttcttttaaa ccttcaattg
     4501 ccccctgcgc gccaatcgcc gttgaggcac ctccggcccc taacagcacc attgttttgc
     4561 ctttgatatc aaaaccgctc tctttaatgg cgcgaatatg gcccgtgccg tcggtgttat
     4621 agccgcgcag gtagccatca tcattaacga tggtgttgat ggcacccacc agtttggctg
     4681 ctggcgttaa ttcatcaaca tattcacacg ccagttgttt gttcggcatt gatataccag
     4741 ttccgcgcat tttgagggct tttaatcctt caattgctcc gggaaaacta tcgttatcca
     4801 cttcaaaggc catataggta aatggcaatc ccgctttttc taaggcttta ttctgcattt
     4861 caggcgataa actgtggcgg ataggatagg ccatcagccc aatcaattcg tattttgcgg
     4921 taacatccat aattaactcc tttaacctct atgcttaatt gtgtttaacc gggtgctaaa
     4981 tttacgctcg ccaaaccgca catcattctc aggaataata aaaacgcggt aatagcggat
     5041 aaaaacaata attgcggtaa tcagggccag cagcgcgaaa gtaaaatcga gaacaatgat
     5101 atattgaagg ccgatgttcg acagataacc ggtaatcagc ggaataacaa agttagccag
     5161 tccacccatc atcatataaa tactggtgac tttggctttg ctttttggga aaaactctga
     5221 catgaccgaa acaccgagct gtaaaatgcc gccagctgct gagaaaccga taacaaaggc
     5281 gccggcatta cacaccagtg gagaagggta caggtagata atggctgctg ttattgttgc
     5341 cagtgcagag ttaaatacat tagcccagat gggccggacc atttttttca gtagtgcggc
     5401 aaaaataaag acacagacca acgagcccat actgtaataa gagatggttt ttaatgcctc
     5461 agcttctgac ataccagcaa aagccatcgc atatttgggc atccacacca caatcacata
     5521 aaaggtcgag aatgcggcta caccgaacag taccgatgaa acaccttcca gccagactaa
     5581 cggtttgctg ttcatttgcg gtaattcatt agctacgctg gcgtccacca actggctggg
     5641 gaatttgctt ttcaacagca tcagcgtgat cagtacaaac agaatacccg gaataatcag
     5701 cccatagccg taccagatat tattgagcaa catatagctc accagcattg ggtagaacat
     5761 ttgcccaaat gacaccatcg ctttaaccag tatgaccgcc gaaccagagg ctttcggaaa
     5821 gcattccatg agcgcggggt agccacccgt atccagcgct gagttagcga tacctacgca
     5881 cactgccaga ccgtaggcga gagttaaatt cgggcaagcg ggaataccaa agaagaatag
     5941 cagatacatt attactgcca ttaatatcac cgcccgacga ccaaacttat cggagatcac
     6001 accgaagaat aaaatactga tcaatcgccc caaaccaata ccggaaatta agtaggcaat
     6061 gcccgcgttg tcagtggaaa acttttccgc cagagatgac atgttttggg caagcgtaat
     6121 aacactaatg ccgtgcagga agtagctgaa gtaaatacaa agaacagcca ggataaatgg
     6181 cgtgctgaaa gccttatttt gagacatttt cacagctcct tgctgaaaga taatttcgtt
     6241 accgtacgta ttgttcttcg acggtttaca taattactat actgaagatg attttccctg
     6301 ggtacatggc ataaagttta tgtttccagg aatattgcat gtcatagttt gtttttgatc
     6361 gcgattttcc ttagttagcg cagaaagagt tatttatccg taagatcttg catgacctga
     6421 aaagaatatt gcattaccca cccggagcga cattttcctt tagcgagtga tgccgcgttt
     6481 ttttgcggct gcgtaagccg ataaacagtg ccagtaaaaa accgatggca gcgatggcgg
     6541 tatcgaacca cataatatcg gcaatacttc tttgggacag atgagccgta atcaacggaa
     6601 tagtaaaggt cgcaatactg cccgcactgt aatagatccc tgtagcttta cctttagcgt
     6661 aagggaaacg ttcagccatt aacgtcaggc caatttgcac aacacctcca gcagaggtaa
     6721 aaccaatgac aaaagcaaat attatcacca cataaaatgt gggatgcagg cagacggtaa
     6781 acagagcaat aaatgagata aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat
     6841 tacgaatgag tggagcggta ataaatacac aaagcagcga atccacggtg tagatactga
     6901 gtagtttaat cgacatagtg tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc
     6961 actggctaac cagataaaac gttgccattg agatatagcc atataaggta taactggcta
     7021 aatcgataat tgaacagcga tgttccgtag agctggtggt tttctttatg acaggtaagc
     7081 gatgacccgg atggggtggg aacgtacaac ggtataaaaa cagagcgtta ataaacataa
     7141 tgcctgcagc aatcataaag gaccaaccga accacagttc agcccacact aacaggctaa
     7201 tgattagcgg taataaaaat tgtccgctgg aaacaaatgc tttaattaaa atattggctg
     7261 tcccaggtga gcgtggaaaa gcttccatca aactgggata agtgcctgca tcgagaaaac
     7321 tgtttgccat tcctgccaga aagccaaaaa cataagcgat aatgatgtta ttggtgtgca
     7381 ggatgccaaa aaagaaggcc atatagcagc acatcccgag catgataaaa gggcggcgac
     7441 caaagcgatc ggataataat cctgcaaaaa gcaagacact taatcgacca atgcccagcg
     7501 atgagataac tatcgagaca cccgcggcat tagtctgcca aagtgtctcc agcgaggcca
     7561 tattcaggct catcaaaatg acgcccatac catgcactag gtaattaaaa tacaacccaa
     7621 gtgcggtagg gtaataggga tttttcatag gttctactta gcaaaggtgc tttaaaaagt
     7681 gtagcgatgt tgtgtcagta tccgttcatt attgttgtaa caacgacaat tcctttttgt
     7741 ggagaaatta acaaagccgc tccagatcgt gtgcatataa ttcagcggct accgattgat
     7801 agatcttcca gtcaataaaa ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg
     7861 ggaaaaaacg catggtgttg ttgccgatgc ggttatttat tttctctatg atagcatgaa
     7921 gatgtttttt tcgttgctga cgcatagcca acagttgctc gactacacga tcaggaatgg
     7981 cgcatttgcc attttcccac tgacgccagc tttcactgtc accggtctgg gcaatccagg
     8041 tcgcgcattc atcaatagtc atggcaaaaa tatggcgaag tgcctggagt tcataagcgt
     8101 tcatcgtgta atggctgcat gaaataaaca ataaaatata aataacatta tcagcctgct
     8161 gacggcttga agagagtcac agtatcttgt gcaacattat tggaaagagt gaggggcgag
     8221 gtagcgaagc ggaaaaatgt taaaaaaaag cccatcgtgg gagatgggca aagactacac
     8281 acagcaattc gttgtttcac tcaggggatt tccatgctta taaatcaata tgttgattta
     8341 taaccgtaag ctaatagtag gcatacggac tttttgcgtc gatcagattc gtctcattag
     8401 tttaaaaact gtaaagaatt tgcgacaggt actaccagct gaatggcggt aagcaggaaa
     8461 atatggggaa gagcaagcaa atatcaatta gcgggggtta acaatcaggc ggcagagccg
     8521 cctgatgaga aattacttat tcggtttctc gatttcgcca agttcttcga gaatttcttc
     8581 cggttggtcc gtcggcggcg ggacttcttc cacccacgcg gcaaacagac gccaggaaac
     8641 ggctaacaga accggaccaa taaacagacc gatcatcccg aaagcaatca aaccaccaat
     8701 aacaccagag aggatcagga tcagcggtaa atcggcaccc atgcgaatta acattgggcg
     8761 gatgacgtta tccagcgtgc caaccacacc gctccacact aacaataccg ttccccaggt
     8821 ggtatcgcca gtccagtaga gccagataat cgccggaatc agtaccggca acgggccaag
     8881 ctggacaagg caggagagga tcattaacac cgttagcaac gttgcataag gtacgccgga
     8941 tacggcgagg ccgataccgc caagcactgc ctgtactaac gccgtcacca ccacacccag
     9001 cgccaccgcg cggatagcct gtgccgccag cagtacggcg gcatctccgc gaacgcctgc
     9061 cagacgggtt gcaaaatggc gaatgccttg tgccacctgt tcaccgcgcc agtacagcag
     9121 ggcactgaag agaagcatca gcgcacaatg caccataaag cgcccgatat gcgccgcctg
     9181 cccaacgaac caggtggtgg tggtgccaat ataagggcgg actttcgcca tgatcgccgt
     9241 cccccccata tccagcaagt tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat
     9301 ggtattaagc cacgccagat cgggtaacgt catgtcgccg ctggaaatgg ctttaattag
     9361 cgggccgctg ccgtcgacga tactgttaac cagcaaagca atagggatga taaacaccat
     9421 cactaataac agcgtcatca ccagaacggc gagagagcgg cggccaaaca tgatcttttg
     9481 caaacgtaac aataccggcc aggtggcgat aaccaccgta ccggcccatg caaagccgag
     9541 aataaagggt tgaacaatcc acagacatgc cacaatcatg atggctaaaa acagcaccga
     9601 aagcagaatt tgtgcgacat ccctgggctg acgaacattt accataacta cttttcacct
     9661 tttttgtgcg tcaaatcgtc gacgcgacgt gaacacgcga aactcacctg cataatgatg
     9721 agcaatttca gcggttttta acaggccgat tctgccctta attctgatgg gcgtgttaga
     9781 aaaaaatgtg gtacaacaac tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt
     9841 tgcatgtgct gcggctacgt tcgttcaccc cagtcactta ctattgtaag ctcctggagt
     9901 tcactcgctt gccgccttcc tgcaactcga aatccataga gtaaataaac atcacctttc
     9961 agtgcgctat tgcagccgcc tgaaagaata gtgtatcgac ctacatcaca gaccgcagga
    10021 aagggtcaat ataatgattc cacagatttc ccaggcaccc ggcgtcgttc aactggtgct
    10081 taattttttg caagagctgg agcaacaagg ttttaccggc gatacggcga caagttatgc
    10141 cgatcgtctg acaatgtcga ccgacaacag tatttaccaa cttctccccg atgcggtggt
    10201 atttccgcgt tcaaccgcag atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta
    10261 ttcatcgctg atctttaccc cccgtggcgg cggcaccggc actaacggtc aggcgctcaa
    10321 ccaggggatt attgttgata tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga
    10381 agagggctgg gtgcgcgttg aggccggggt gataaaagat caactcaatc agtacctgaa
    10441 accgttcggc tacttttttg cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg
    10501 gatgatcaat accgatgctt ctggtcaggg atcgctggtc tatggcaaaa cgtcggatca
    10561 cgtacttggc gtacgtgcgg tgttgttggg gggcgatatt ctcgatacgc aacctttacc
    10621 cgtcgaactg gcggaaacgc tgggtaaatc caataccaca atcgggcgaa tttataacac
    10681 ggtttatcaa cgttgccgtc agcaacgcca gttaatcatc gacaacttcc ccaaacttaa
    10741 ccgctttctt accggttacg atctgcgtca tgtctttaac gatgagatga ccgagttcga
    10801 cctgacgcgc attctgacgg gttcagaagg gacgctggcc tttattaccg aagcgcggct
    10861 ggatattacg cccttgccta aagtgcgccg tctggtgaac gtcaaatatg actcttttga
    10921 ctccgcgctg cgtaacgcgc cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt
    10981 ggactcaaaa gtgctgaatc tggcgcggga agatattgtc tggcattccg tcagcgagtt
    11041 gattaccgat gtgcctgaca aagagatgct cgggctgaac attgtggagt ttgctggtga
    11101 tgatgaggcg ctgattgacg agcgagttaa tgctctctgt gtgcggcttg atgagctgat
    11161 tgccagccaa caggcaggtg tgattggctg gcaggtgtgc cgcgagctgg cgggtgttga
    11221 acgtatctat gcgatgcgta aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc
    11281 taagccaatt ccgtttgctg aggatacctg cgtaccgccg gaacatctgg ccgattatat
    11341 tgctgaattt cgcgcgctgc tcgacagcca cggcttaagc tacggtatgt tcggtcacgt
    11401 cgacgcaggt gtcttgcacg tccgtccagc gctggatatg tgcgatcctc aacaagagat
    11461 tttgatgaag caaatctctg atgacgtagt ggcgctgact gcgaaatacg gtggtttgtt
    11521 gtggggcgag cacggcaaag gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga
    11581 actttttgca gaactgcgca aagtgaaagc ggcatttgac ccgcataacc gactcaaccc
    11641 agggaagatt tgcccgccag aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa
    11701 gcgcggtaca ttcgatcggc agatccccat tgcggtacgc cagcagtggc gcggtgcgat
    11761 ggagtgtaac ggcaacggtt tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc
    11821 gatgaagatc acccagaacc ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga
    11881 atggctgcgt ttgttggcgg atcgcggcgt tgatccactc aaactggaac aagaactgcc
    11941 tgaatccggc gtcagtttgc ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa
    12001 taaaggcgaa tatgacttct cacacgaagt caaagaggcg atgtcgggct gtctggcctg
    12061 taaagcgtgt tcgacccagt gccccatcaa aattgatgtg ccggagtttc gctctcgttt
    12121 tctgcagctc tatcacaccc gttatttacg cccgctgcgc gaccacctcg tcgctacggt
    12181 cgagagctac gcgccgctga tggcacgcgc gccgaaaacc tttaacttct tcattaacca
    12241 gccgctggtg cgcaaactct cggaaaaaca tatcggcatg gttgatttgc cgctgctgtc
    12301 ggtcccctcg ctacaacaac aaatggtggg gcatcgctcg gcaaacatga cgctggaaca
    12361 gcttgaatct ctcaatgcag agcagaaagc gcgcacagtg ttggtggtgc aggacccctt
    12421 taccagctat tacgatgcgc aagtggtggc ggattttgtc cgtctggtcg aaaaattagg
    12481 tttccagcct gtgttactgc cattttcgcc aaatggcaaa gcccagcata ttaaaggttt
    12541 tcttaatcgt tttgcgaaga cggcgaaaaa gacggcggat ttcctcaacc gtatggcgaa
    12601 gctgggtatg ccaatggtgg gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata
    12661 taaactggcg ctgggcgagg aacgtggcga gtttaacgtc ttactggcga atgaatggct
    12721 ggcaagcgca cttgagtcac agccagtggc tacagtcagc ggtgaatcat ggtatttctt
    12781 tggtcactgt accgaagtta ccgccttgcc gggtgcgcca gcacaatggg ccgcgatatt
    12841 tgcccgtttt ggcgcgaaac tggaaaatgt cagcgtgggt tgctgcggca tggcagggac
    12901 ttacggacat gaagcgaaaa accataaaaa ttcgctcggg atctatgagt tatcctggca
    12961 tcaggctatg cagcgactgc cgcgtaaccg ctgtctggcg accggatatt cctgccgtag
    13021 ccaggtaaaa cgggttgaag gcacgggggt acgccatcct gtgcaggctt tactggagat
    13081 tattaaatga tatggaaacg gaaaatcacc ctggaagcac tgaatgctat gggtgaagga
    13141 aacatggtgg ggttcctgga tattcgcttt gaacatattg gtgatgacac ccttgaagcg
    13201 acaatgccag tagactcgcg gacaaagcag cctttcgggt tgctgcatgg aggagcatcc
    13261 gtggtactgg ccgaaagtat cggttccgtt gccggttatt tatgtaccga aggtgagcaa
    13321 aaagtggttg gtctggaaat caatgctaac cacgtccgct cggcacgaga agggcgggtg
    13381 cgcggggtat gcaaaccgtt gcatctcggt tcgcgtcacc aggtctggca gattgaaatc
    13441 ttcgatgaga aagggcgttt gtgctgttcg tcacgattga cgaccgccat tttgtgacgt
    13501 tgtcaccaga aaagtgtgac gcatattgcc tttggtcatt atctgcacag taaacggtgg
    13561 tatactgtgc aggtcttgtc taaaagcgag gatgctatgt ctactcaact tgatcccacc
    13621 cagctggcca ttgaattttt acgccgtgac cagagcaatc tttcacctgc ccaatatctc
    13681 aaacgtctga aacaactaga acttgaattt gctgacctgc ttactctctc ttctgctgaa
    13741 ttaaaagaag aaatctactt tgcctggcgt ctgggcgtgc attgatcctg acttaatgcc
    13801 gcaagttcgc caaatcgacc tggattaacc tccgtccagg ttcgatgacc ttcttaatac
    13861 tgcttatcta ccgcctgata ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg
    13921 gcgggttagc tttatgagat tattcttatc gccccttcaa gagctaagcc actgagagtg
    13981 ccggagataa gcgccggatg gggtagaaac ccttaagcct gtgttgcaca gacttaaggg
    14041 ttttcttatt tctgaacgct gcactttttt acctatctct taaatagtca ttttcaatac
    14101 aactttttct ttttcatcac agccttaatg ctccttagat aataaccatt atctaacaat
    14161 gagataccta attcttagcg tgcctgttaa cccacacatc agggtctatg cttattaaaa
    14221 gcaggcaaaa agcatgtttt acggtaaagc ccctgcgttt gctgggttgt actgataatc
    14281 attatcacta acatgctgtt atacgctgaa agcgatgaag tgaggtaaat cgatggacat
    14341 gcattcagga acctttaacc cacaagattt cgcctggcaa ggcttaacgc tgacacccgc
    14401 agcggcgata cacatccgtg agctggtggc aaagcagccg ggtatggtcg gcgtgcgctt
    14461 aggcgtgaag caaacgggct gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc
    14521 ggacaaagac gatctgctgt ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaagc
    14581 gatgccgttt attgatggca cggaagtcga tttcgttcgt gaaggactta atcagatatt
    14641 caaatttcac aaccctaaag cccagaatga atgtggctgt ggcgaaagct ttggggtata
    14701 ggcggtacta tgtctcgtaa tactgaagca actgacgatg tcaaaacctg gaccggcggc
    14761 ccgctgaatt ataaagaagg attcttcacc cagttagcca ccgatgagct ggcaaagggg
    14821 ataaacgaag aggtggtgcg cgcaatttcg gcgaagcgta atgagccgga gtggatgctg
    14881 aagtttcgtc taaacgccta tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa
    14941 gcgcactacg acaagctgaa ttatcaggat tacagctact actcagcacc atcgtgcggt
    15001 aattgtgacg acacttgcgc gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc
    15061 tttttaagta aagaggtgga ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc
    15121 aaagaggtgg cggtggatgc cattttcgac tcagtttcgg ttgccactac ttatcgcgaa
    15181 aaactggcgg agcagggaat tattttctgt tcctttggtg aggcgatcca cgatcacccg
    15241 gaactggtgc gtaaatatct cggcaccgtg gtgccgggga atgacaactt ctttgccgcg
    15301 cttaatgcgg cggtagcctc tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc
    15361 ccgatggaac tttccaccta ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc
    15421 accattctgg tggccgacga agacagctac gtcagctaca ttgaaggctg ttccgctccg
    15481 gtgcgtgaca gctatcagtt acacgcggca gtggtggaag tcatcatcca taaaaacgcc
    15541 gaggtgaaat attccacggt acaaaactgg ttccctggcg ataacaacac cggcggtatt
    15601 ctcaacttcg tcaccaagcg tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg
    15661 caatcagaaa ccgggtccgc gattacgtgg aaatatccca gctgcatttt gcgcggcgat
    15721 aactccattg gtgagtttta ctcagtggcg ctgaccagcg gtcatcagca agcggatacc
    15781 ggcaccaaga tgatccacat cggtaaaaac accaaatcga ccattatctc gaaagggatc
    15841 tctgccggac atagtcagaa cagttatcgc ggcttagtga aaatcatgcc gacggcaacc
    15901 aatgcgcgca atttcactca gtgcgactca atgctgattg gcgctaattg tggggcgcat
    15961 accttcccgt atgtcgaatg tcgcaataac agcgcacaac tggagcacga ggcaacgaca
    16021 tcacgtattg gtgaagatca actgttttac tgcctgcaac gcgggatcag cgaagaagac
    16081 gccatctcga tgattgttaa cggtttctgc aaagacgtgt tctcagaact gccgctggaa
    16141 tttgccgttg aagcacaaaa actcctcgcc atcagtcttg aacacagcgt cggataagga
    16201 ataaacatgt taagtattaa agatttacac gtcagcgtgg aagataaagc tatcctgcgt
    16261 ggattaagcc tcgccgttcg tcccggcgaa gttcacgcca ttatggggcc aaacggttcg
    16321 ggcaaaagta ccttatcggc aacgcttgcc gggcgcgaag attatgaagt gacgggcggc
    16381 acggttgagt tcaaaggcaa agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa
    16441 ggcatcttta tggccttcca gtatccggtg gagattccag gcgtcagtaa ccagtttttc
    16501 ctgcaaacgg cacttaatgc agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt
    16561 gattttcagg atttgatgga agagaaaatc gctctcctga agatgccgga agatttatta
    16621 acccgttcgg taaacgttgg tttttccggc ggcgagaaaa agcgcaacga tattttgcaa
    16681 atggcggtgc tggaaccgga gttatgcatt cttgatgagt cggactccgg gctggatatt
    16741 gacgcattaa aagtggtcgc cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc
    16801 atcattgtta cgcactacca acgcattctc gactacatca agcctgatta cgtccatgtg
    16861 ctatatcggg gacgaattgt gaaatccggc gatttcacgt tggtcaaaca actggaggag
    16921 cagggttatg gctggcttac cgaacagcag taacgcgctg caacagtggc atcacttgtt
    16981 tgaagctgaa gggacaaaac gctcaccgca agcacagcag catttacaac aattgctgcg
    17041 taccggactg ccgacacgta aacatgaaaa ctggaaatat acgccgctgg aagggctgac
    17101 caatagccag tttgtcagca ttgcgggaga gatatcccca cagcagcgag atgccttagc
    17161 gttaacgtta gacgctgtgc ggctggtatt tgtcgatgga cgttacgtgt cagcactgag
    17221 cgatgcgact gaaggcagcg ggtatgaagt gagcattaac gacgaccgtc agggggtacc
    17281 cgacgctatt caggcggaag tgtttctgca tttgacggaa agcctggcac aaagcgtgac
    17341 gcatatcgcc gtgaagcgcg gtcaacggcc ggcaaagcca ttgctgttaa tgcatattac
    17401 ccagggcgtg gcaggtgaag aggtcaacac tgcccattac cgacatcatc tggatctggc
    17461 ggaaggtgcc gaagcaacgg tgatcgaaca ttttgtcagc cttaatgatg ctcgtcactt
    17521 caccggcgca cggttcacta tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct
    17581 ggcgtttgaa aacccggtca gtcaccactt tgcccataac gatttgttgc tggctgacga
    17641 tgccaccgca tttagccata gtttcctgct gggtggcgca gtgttacgac acaacaccag
    17701 tacgcaactc aatggcgaaa acagcacgct gcggatcaat agcctggcga tgccggtgaa
    17761 aaacgaggtg tgtgataccc gtacctggct ggaacacaat aaaggttttt gtaacagccg
    17821 acagttgcac aaaactatcg tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa
    17881 cgtcgcgcag cacgccatca aaacggatgg tcagatgacc aacaataatc tgctgatggg
    17941 caaactggca gaagtggata cgaaaccgca gctggaaatc tatgcagatg atgtgaaatg
    18001 cagccacggc gcgacggtgg ggcgtattga tgatgaacag atgttctatc tgcgctcgcg
    18061 cgggatcaat cagcaggatg cccagcagat gatcatttac gcctttgctg ccgaactgac
    18121 ggaagcactg cgtgatgagg ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct
    18181 gccaggaggt gcaagatgac tttttccgtc gacaaagtgc gggccgactt tccggtgctt
    18241 tctcgtgagg taaacggttt gccgctggct tatctcgaca gcgccgccag tgcgcagaaa
    18301 ccgagccagg tgattgacgc cgaggccgag ttttatcgtc atggctacgc ggcggtgcat
    18361 cgcggtattc ataccttaag cgcccaagcg accgagaaaa tggagaacgt gcgcaagcgg
    18421 gcatcgctgt ttattaatgc ccgttcggcg gaagagctgg tgttcgtccg cggcacgacg
    18481 gaagggatca atctggtcgc caatagctgg ggtaacagca atgtgcgggc gggcgataac
    18541 atcatcatca gtcagatgga gcaccacgct aacattgttc cctggcagat gctttgcgca
    18601 cgcgttggcg cagagctgcg tgtgatcccg ctcaatcccg acggtacgtt gcaactggag
    18661 acgctgccta cgctgtttga tgagaaaact cgcctgctgg caattactca tgtctccaac
    18721 gtgcttggca cagaaaatcc actggcggaa atgatcacgc ttgcgcacca gcatggcgca
    18781 aaagtgctgg tggatggcgc tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg
    18841 gattgcgact tttacgtgtt ctccgggcat aaactgtatg gccccaccgg aattggcatt
    18901 ctttatgtga aagaagcctt gttgcaggag atgccgccgt gggaaggggg cggttctatg
    18961 atcgccaccg tcagcctgag tgaaggcaca acctggacca aagcaccttg gcggtttgaa
    19021 gccggtacac ccaataccgg gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg
    19081 gcgctggggc ttaataacat agccgagtat gaacagaatc tgatgcacta cgcgctatca
    19141 cagctggaat ctgtaccgga tctcactctc tatggtccgc agaacaggct tggcgttatt
    19201 gcttttaatc ttggtaaaca ccacgcctat gatgttggca gttttctcga taactacggc
    19261 attgctgtgc gtaccggaca tcactgcgct atgccattaa tggcctatta caacgtccct
    19321 gctatgtgtc gggcgtcgct ggccatgtat aacacccatg aagaagtgga tcgtctggtg
    19381 accggactgc aacgtattca ccgtctgctg ggataacagg gaggcactat ggctttattg
    19441 ccggataaag aaaaattgct gcgtaatttt ttacgctgcg ccaattggga agagaaatat
    19501 ctctacatta ttgagctggg ccagcgtctg ccagaattac gcgacgaaga cagaagccca
    19561 caaaatagca ttcagggctg ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag
    19621 ggaattattg aattgcaggg cgacagcgat gcggcgattg tgaaagggct tattgcggtc
    19681 gtctttattc tctacgatca gatgacgccg caggatattg tcaatttcga tgtgcgtccg
    19741 tggtttgaaa aaatggcgct cacccaacat ctcaccccat ctcgttcaca aggtctggaa
    19801 gcgatgattc gcgcaattcg cgccaaagcc gctgcactta gctaaactaa agggacagct
    19861 ttcatccggt tggctcacat tgccggatgg tcaatgttta cactgacctg cacaggagtg
    19921 tttcggtttc aggtgaacat aagcgccatc cgacgtttca gcgtgagtct ccggcaaata
    19981 caggaggttt acaatgaaac gcgcgtcttt gcttacactc acgcttatcg gcgcttttag
    20041 cgccatccag gctgcctggg cggttgatta tccgctacca ccaaccggaa gccgactggt
    20101 tgggcaaaat caaacgtata cggtgcagga aggggataaa aaccttcagg ccatcgcccg
    20161 acgttttgat actgcggcaa tgttgatcct tgaagccaat aacactatcg ccccggtgcc
    20221 aaaacccggt acgacgataa ctattccttc gcaactgtta ttacctgatg cgccgcgtca
    20281 ggggattatc gttaaccttg cagagctgcg cctttattat tatccgccgg gagaaaatat
    20341 cgtgcaggtt tatccgatag gtattggctt gcaggggctg gaaacgccgg tgatggaaac
    20401 gcgtgtgggg cagaaaatcc ctaacccaac ctggacgcct actgcaggca ttcgtcaacg
    20461 ttcgctggag cgtggtatta aattaccgcc agtcgttcct gccggaccaa ataacccgct
    20521 aggacgttac gcactgcgcc tcgcgcatgg taatggcgaa tacctcattc atggcaccag
    20581 tgcgccggac agcgtcggtt tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga
    20641 tattaaagcc ttgttctcca gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc
    20701 tgtgaaatat tccgtggagc ctaacgggat gcgttatgtt gaagtacatc gaccactatc
    20761 ggcagaagaa cagcagaacg ttcagacaat gccatacaca ctgccagcag gctttacgca
    20821 atttaaagac aataaggctg tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc
    20881 agggtatccg gtttcggtga gcagtggagc aactcccgca gccagcaatg cgccttcagt
    20941 agagtcagcg cagaatggtg aaccagagca agggaatatg ttacgcgcga cgcagtagcg
    21001 gtaaacggca gacaaaaaaa tggcgcacaa tgtgcgccat ttttcacttc acaggtacta
    21061 ttacttgcgg tatttagtag ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc
    21121 tttagcagcc tgaacgtcgg aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt
    21181 agcgttcaga gtctgaacgt cagaagacag ctgatcgatt ttagcgttgc tggagcaacc
    21241 tgccagcaga gtagaaccca ggattaccgc gcccagtacc agtttagtag ctttcattat
    21301 taataccctc tagattgagt taatctccat gtagcgttac aagtattaca caaagttttt
    21361 tatgttgaga atattttttt gatgggaagg cacttatttt tgatcgttcg ctcaaagaag
    21421 catcgaacgc tgggttttag ataaaaaaat tgagagaaaa caggatgctt ccatcggatt
    21481 catcttaga
//
LOCUS       DAZGOB010000077        21186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGOB010000077 DAZGOB010000000
VERSION     DAZGOB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.077"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            244..501
                     /locus_tag="ACHLIF_003101"
     CDS             244..501
                     /locus_tag="ACHLIF_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO9607074.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGKPLSEAERQRAASARKRSVCKEI
                     KVFVRPELKNYLTSLCAEEGVTQAELIERLIEKEACHRNMM"
     gene            complement(542..688)
                     /locus_tag="ACHLIF_003102"
     CDS             complement(542..688)
                     /locus_tag="ACHLIF_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFO9607075.1"
                     /translation="MGPGNLSDSPKLKKIIGVFAFLAPVNPHQNQFPATLRRGQPQNS
                     LNDQ"
     gene            735..809
                     /gene="tap"
                     /locus_tag="ACHLIF_003103"
     CDS             735..809
                     /gene="tap"
                     /locus_tag="ACHLIF_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO9607076.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            802..1659
                     /gene="repA"
                     /locus_tag="ACHLIF_003104"
     CDS             802..1659
                     /gene="repA"
                     /locus_tag="ACHLIF_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO9607077.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            2570..2854
                     /locus_tag="ACHLIF_003105"
     CDS             2570..2854
                     /locus_tag="ACHLIF_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO9607078.1"
                     /translation="MQMKNNTTQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            2854..3129
                     /locus_tag="ACHLIF_003106"
     CDS             2854..3129
                     /locus_tag="ACHLIF_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9607079.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            3236..3427
                     /locus_tag="ACHLIF_003107"
     CDS             3236..3427
                     /locus_tag="ACHLIF_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607080.1"
                     /translation="MNYDEITKITAERIRDYMTEAVNTDSKAVAEMFHNAAWGVRTLW
                     FELVTKIDMDIHKKNRYAS"
     gene            3779..4126
                     /locus_tag="ACHLIF_003108"
     CDS             3779..4126
                     /locus_tag="ACHLIF_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO9607081.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(4144..4734)
                     /locus_tag="ACHLIF_003109"
     CDS             complement(4144..4734)
                     /locus_tag="ACHLIF_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607082.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPATGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESTNP"
     gene            complement(4734..4985)
                     /locus_tag="ACHLIF_003110"
     CDS             complement(4734..4985)
                     /locus_tag="ACHLIF_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607083.1"
                     /translation="MDEERVFSLSYEQLTRIAEKNIRQCGLDSQIARSISELKASALL
                     WLWYELAIHGAPQNNHAQAQRINTDYQRLKKFIWPEGDS"
     gene            5556..5870
                     /locus_tag="ACHLIF_003111"
     CDS             5556..5870
                     /locus_tag="ACHLIF_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607084.1"
                     /translation="MGKPTFRSFYDVVRELEDVYGHKELWLYSGAAYATPTEMINARH
                     NWKSPKILKRNGRMVAERMDNSDSWQLVGDYKKPLFQHCAPPWQSCQIDDYFKGYYII
                     AP"
     gene            5923..6702
                     /locus_tag="ACHLIF_003112"
     CDS             5923..6702
                     /locus_tag="ACHLIF_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607085.1"
                     /translation="MGSMKELLFEMQEERRDEWIAENYPDAEEGTPEWDAAAQEYSWF
                     QDWMEEAAEQQYFEASLASIPDRLQDAKAELDELESLMQFNQPRIVERMAYVHCVSVL
                     DSFLMYSARALLSHPPHLQKFLHEADSLVPNKEDRRKLLASKWVEQEPDKDTPEKVYT
                     WRAQSLVAKKTFQSHKVIGRYFSRMLTTPHEWPLEEIKGVIKTRNALVHRNGVTESLE
                     PVYISSGSVQNAICTVRAFITVAAETLLQEDALYRTDDGIF"
     gene            6747..7532
                     /locus_tag="ACHLIF_003113"
     CDS             6747..7532
                     /locus_tag="ACHLIF_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO9607086.1"
                     /translation="MSMTSLPANCIPVSNTSLPVAIDYPAALALRQMALVQDELPKYL
                     LAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQ
                     RTEKAARTAGRMPAGQQTHRLVPLSDAWFVSQLQTMVATLKIPMERRNKRTGRTEKAR
                     IWEVTDRTVRTWIGQAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLM
                     GHKSISSTEVYTKVFALDVAARHRVLFSMPESDAVTMLKNRHA"
     gene            complement(7670..7945)
                     /locus_tag="ACHLIF_003114"
     CDS             complement(7670..7945)
                     /locus_tag="ACHLIF_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO9607087.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQC
                     RTTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     gene            complement(7939..8583)
                     /locus_tag="ACHLIF_003115"
     CDS             complement(7939..8583)
                     /locus_tag="ACHLIF_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFO9607088.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAA
                     KHHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAH
                     QIIAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLE
                     YLEEFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAARAEIEHLMNEVFGPW"
     gene            8812..9783
                     /gene="parM"
                     /locus_tag="ACHLIF_003116"
     CDS             8812..9783
                     /gene="parM"
                     /locus_tag="ACHLIF_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HFO9607089.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQ
                     TFNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLAPQPVSLTVTL
                     PISEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVMD
                     KVGQFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSD
                     TSFLVADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVN
                     RVYVVGGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     gene            9788..10180
                     /locus_tag="ACHLIF_003117"
     CDS             9788..10180
                     /locus_tag="ACHLIF_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO9607090.1"
                     /translation="MDDERKRKKFTLYLHPEKAADFQTLEAIESVPRSERGELFRNAF
                     ISGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRAVLTELGALQ
                     SAEKMPPSATDSVQEAMNDVRLKMQKLF"
     gene            complement(10185..11456)
                     /locus_tag="ACHLIF_003118"
     CDS             complement(10185..11456)
                     /locus_tag="ACHLIF_003118"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFO9607091.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPSYTTQWTEIPIASF"
     gene            complement(11456..11893)
                     /gene="umuD"
                     /locus_tag="ACHLIF_003119"
     CDS             complement(11456..11893)
                     /gene="umuD"
                     /locus_tag="ACHLIF_003119"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO9607092.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDMVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(11890..12138)
                     /locus_tag="ACHLIF_003120"
     CDS             complement(11890..12138)
                     /locus_tag="ACHLIF_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO9607093.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERIMSLLEEIWQDDSWLPAA"
     gene            12556..13458
                     /locus_tag="ACHLIF_003121"
     CDS             12556..13458
                     /locus_tag="ACHLIF_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO9607094.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATDEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGNWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            13455..13766
                     /locus_tag="ACHLIF_003122"
     CDS             13455..13766
                     /locus_tag="ACHLIF_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607095.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARQERCLWSVSRV
                     RRARDGRFYCSRLAPVTVYASLSPFSDERPSSRFRGIFSPSERRRLRYSTVGLTRYRT
                     R"
     gene            13843..14526
                     /locus_tag="ACHLIF_003123"
     CDS             13843..14526
                     /locus_tag="ACHLIF_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO9607096.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVVGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            14527..14748
                     /locus_tag="ACHLIF_003124"
     CDS             14527..14748
                     /locus_tag="ACHLIF_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607097.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            14762..15160
                     /locus_tag="ACHLIF_003125"
     CDS             14762..15160
                     /locus_tag="ACHLIF_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO9607098.1"
                     /translation="MYGTCETLCRELAAKYPGEIQALADGMDISLSDHEIRTVLARLE
                     DIPEDQRTESGISSAAVMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDY
                     GLAVPECVTRRQAVVNQARTLLKNNRHEND"
     gene            15206..15982
                     /locus_tag="ACHLIF_003126"
     CDS             15206..15982
                     /locus_tag="ACHLIF_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607099.1"
                     /translation="MNENITLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIY
                     VLLDAPRLATLLINRHGGALPPLLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLAEDEIRAVLDAVRDAVRCVSYQVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSWLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            16400..16825
                     /locus_tag="ACHLIF_003127"
     CDS             16400..16825
                     /locus_tag="ACHLIF_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9607100.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIIVTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            16872..17294
                     /locus_tag="ACHLIF_003128"
     CDS             16872..17294
                     /locus_tag="ACHLIF_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO9607101.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            17291..17482
                     /locus_tag="ACHLIF_003129"
     CDS             17291..17482
                     /locus_tag="ACHLIF_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607102.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(17755..18003)
                     /locus_tag="ACHLIF_003130"
     CDS             complement(17755..18003)
                     /locus_tag="ACHLIF_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607103.1"
                     /translation="MSRDPPQRRRARARNGVAPYIPAAFCRDARSAGHGATEAARPER
                     SLQARGFHASDRSGSPKPRQRVRRRLAGAPATACRSRA"
     gene            18521..18751
                     /locus_tag="ACHLIF_003131"
     CDS             18521..18751
                     /locus_tag="ACHLIF_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607104.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            18803..20164
                     /locus_tag="ACHLIF_003132"
     CDS             18803..20164
                     /locus_tag="ACHLIF_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO9607105.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYISDMKITEI
                     PQK"
     gene            20212..20775
                     /locus_tag="ACHLIF_003133"
     CDS             20212..20775
                     /locus_tag="ACHLIF_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO9607106.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            20801..21013
                     /locus_tag="ACHLIF_003134"
                     /pseudo
     CDS             20801..21013
                     /locus_tag="ACHLIF_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348622.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4883 a         5463 c         5769 g         5071 t
ORIGIN      
        1 gaggtatttc aggggcagac acccgacatg ccagaaacag ccggtcccgc ccggggccgg
       61 ctcccgggtt aaggcatttc ctgttttttc agccatttcg caacaaaaat cacattaaac
      121 aacagtaatc cccgttgatt tgtgcgccaa cacagatctt catcacaatt ctcaagtcgc
      181 tgatttcaaa aaactgtagt atcctctgcg aaacgatccc tgtttgagta ttgaggaggc
      241 gagatgtcgc agacagaaaa tgcagtgact tcctcatctg gcgcaaaacg agcatacaga
      301 aagggtaaac ctttgagtga agcagaacga cagagagcag cttctgcgcg caaacgatct
      361 gtatgtaaag agatcaaggt tttcgtcaga ccagaactga aaaactacct taccagtctg
      421 tgtgcagagg aaggggtaac tcaggcagag ttgatagaaa ggcttatcga gaaagaggcc
      481 tgtcaccgga atatgatgtg atgggatcac attcttgcta gttttttctg tcagtgctag
      541 attactgatc gtttaaggaa ttttgtggct ggccacgccg taaggtggca gggaactggt
      601 tctgatgtgg atttacagga gccagaaaag caaaaacccc gataatcttc tttaactttg
      661 gcgagtcaga aagattaccg gggcccactt aaaccgtata gccaacaatt cagctatgcg
      721 gggagtatag ttatatgccc ggaaaagttc aagacttctt tctgtgctca ctccttctgc
      781 gcattgtaag tgcaggatgg tgtgactgat cttcaacaaa cgtattaccg ccaggtaaag
      841 aacccgaatc cggtgttcac accccgtaaa ggtgccggaa cgctgaagtt ctgcgaaaaa
      901 ctgatggaaa aggcggtggg cttcacctcc cgttttgatt ttgccattca tgtggcacat
      961 gcccgttcga agggcctgcg ccggcgtatg ccgccggtgc tgcgccgcag ggcgattgat
     1021 gcgctgctgc aggggctgtg tttccactat gacccgctgg ccaaccgcgt ccagtgttcc
     1081 atcaccacgc tggccattga gtgcggactg gcgacggagt ctgctgctgg aaaactctcc
     1141 atcacccggg ccacccgggc cctgacgttc ctgtcagagc tgggactgat tacctaccag
     1201 acggaatatg acccgcttat cgggtgctac attccgaccg atatcacgtt cacaccggcg
     1261 ctgtttgccg cccttgatgt gtctgaggat gcagtggcag ctgcgcgccg cagccgtgtg
     1321 gtatgggaaa acagacagcg caaaaagcag gggctggata ccctgggtat ggatgaactg
     1381 atagcgaaag cctggcgttt tgtccgtgag cgtttccgca gttaccagac agagcttaag
     1441 tcccacggca tgaaacgtgc ccgtgcgcgc cgcgatgcag gcagaacccg ccaggacatt
     1501 gtcaccctgg tgaaacggca actgacgcgt gaaatcgcgg aagggcgttt ccggggaagc
     1561 ctggaggccg tgaagcgcga aatcgaccgt cgtgtgaagg aacgcatgat aatgtcgcgg
     1621 aacaacaact acacacggct ggccactgcc tccccctgag ttttacctca tctgaataat
     1681 ccggcctgcg ccggaggcat ctgcacgcct gaagcctgtc agcgaacaaa aaaacagcac
     1741 cacatacaaa aaacaatctc atcatcccgc ttctggtgca tccggtcccc ctgtttttga
     1801 tacaaaacac gcctcacaga cggggaattt tgcttatcca cattaaactg caagggactt
     1861 ccacataagg ttacaaccgt tcatgtcata aagcgccagc cgccagcctt acagggtgca
     1921 atgtatcttt taaacacctg tttatatctc ctttaaacta cttaattaca ttcatttaaa
     1981 aagaaaacct gttcactgcc tgtcctgtgg acagacagat atgcacctcc caccgcaagc
     2041 ggcgggcccc aaccggagcc attttagtta caacactcaa atacaagcac cgggaaaacc
     2101 ccggtccagc gcagaaccga aaccacaaag cccctccctc ataacttaaa agcggccccg
     2161 ccccggccca aagggccgga acagagtcgc ttttaattat gaatgttgta actaagaatc
     2221 atcgtcgctg tcagtcttct ggctggaagt atcgagtaca cgctcgtaag cggccctcac
     2281 ggcccgctaa cgcggagata cgccccgact gcgggtaaac ccttgtcggg accactccga
     2341 ccgcgcacag aagcattttc atcgctgaca ggccacatgg cctcgcaggg ccggagtggg
     2401 actggtgata ttcggacgac cgtaccggct tacgccgggc ttcggcggtt ttacacctgt
     2461 gctacatgta acctccgtat gccgccttac atgcgctgac gcgcattatt taccttgttc
     2521 cgtctgaaag taatcactat gattaaatat gattaacaac taatcggata tgcaaatgaa
     2581 aaacaatacc acacaagcaa caaaagtaat taccgcgcat gtgccattac ctatggctga
     2641 taaagtcgac cagatggccg ccagactgga acgctcccgg ggctgggtta tcaaacaggc
     2701 gctttctgca tggcttgccc aggaggagga gcgtaatcgc ctgacgctgg aagccctgga
     2761 cgatgtgaca tccggacagg ttatcgacca tcaggctgta caggcctggg cggacagcct
     2821 cagtactgac aatccgttac cggtgccacg ctgatggaac tgaagtggac cagtaaggcg
     2881 ctttctgatt tggcgcggtt atatgatttt ctggtactgg ccagtaaacc tgctgccgcc
     2941 agaacggtgc agtccctgac acaggctccg gtcattctgt taactcatcc acgtatggga
     3001 gaacagttgt ttcagtttga acccagggag gtcagacgga tttttgctgg cgagtacgaa
     3061 atccgttacg aacttaatgg ccagactatt tatgtattgc gcctgtggca cacacgagaa
     3121 aacaggtaga ttgtgtgtgg ggacaacttt aacatagcct gcaggtatta attctggagc
     3181 attgttatca ggccagatat tttgcattcc attaaataat cttgtaggag ataaaatgaa
     3241 ttacgatgaa ataacgaaaa ttaccgctga acgtattcgc gactacatga ccgaagcagt
     3301 taatacagac tccaaagctg ttgcggaaat gtttcataac gctgcatggg gagtgcgcac
     3361 attgtggttt gaactcgtca caaaaatcga tatggatata cataaaaaga acaggtatgc
     3421 gagttagaag gaaaatcgaa atgcagcatg aagagtttca gaaaatgaca gagcgcgagc
     3481 aagtgccttt attgaaatcg ccagcatgat gttcagcggc ttcgccttgg tattttcttt
     3541 cgccccttct atgccctgcc ggacattgta taaatgtaac tctgttatac acgttcgtaa
     3601 tcggccctca cggccagctt acgcggatta agataatatt tcctgttcat cagccagcct
     3661 cctgttctcc ttttcttttg tcagggtacc ggtgcatgac aacataatca caacatgata
     3721 ataataatca ataacaataa gctgtgtcac gtttacatca tggaacggag gtgctcctgt
     3781 gacctttgag cagaaaaaag cccgggccat tgccctgatg gacagcaaaa agatgtggcg
     3841 gagcaattat gcccctcctc ttctgcgtat tctctggcgg ctgggtatcc ggcttccgcc
     3901 attacccttt atgccgttct ggcaggtcac ggtgctgacg gggggcttat ggggcatttc
     3961 ctggggatgt gcaatgtggt ttatctactg ggggccgtca ggaatggtag caggtgaggc
     4021 cattattata agtatcacgg gtggtttttt gtccggactg tgcatggcct ctttccactg
     4081 gtggcgcagg aaagttaacc ggctgccacc atgggatgat gtgtaaaccg tgcaggcatg
     4141 tttttacggg ttggtgcttt cattaacctg cacacgtacc tccattcctg catcgtgaca
     4201 ggcctgcagc cactgcgcca cttccagtgg atcgccctcc cggcgtacca ctctgccttc
     4261 tttattccat aactgcagac aggtgctgcc gtcgagacgc accacaaaat ccccacggca
     4321 ggcctgatac gttatgccgt tcagctccac cgccagatca gtggcagaag tgccgtctgc
     4381 gggcagacct gttgccggga ccagcggcag tacgacgact tctgccgcac gctgttctgc
     4441 ccggaggcgg tcctgttctg ccagcagcag aggctgtgcc acggcccggc cggcgtccgt
     4501 cagctctact gccagctgca gattcggggc gcgcagggta cgcagccagc cggcatcctc
     4561 cagacgacgg caggaagcgc gcaggtttgg cccgtagacc ggggcttccc cgcttcgctc
     4621 cagcacccgt tctatgtccc gcgtcagcac ggggcccggc cgtttttcat caagggcagc
     4681 cagcacaatc agcactctcc gctgcagtgg cgatggtcgg cggctctgag tcattaactg
     4741 tcaccctccg gccagatgaa ttttttgagg cgctgataat cggtattgat gcgttgggcc
     4801 tgtgcgtgat tgttctgtgg cgcaccatga atggccagtt cataccacaa ccacagcaga
     4861 gctgaggcct tcagttcact gatgctcctg gcgatctggc tgtccagccc acattgacgg
     4921 atattttttt ccgctattcg ggttagttgc tcatagctga gtgaaaacac cctttcttca
     4981 tccatcctgc acgctccatc tgataatgtt ctgacaggta aaaataatat tttgtatgat
     5041 ttgtgtgctc ttgatttttg ataatatcag atatggtttt atattaaatg atgaaaatca
     5101 tatctgatat gatttttatg tgaattgttt aattatcata ttgcatgccc gttccctgcc
     5161 gcgcagatcc ggtttccggc ggttacggat cttcctcccc ctccgcacag cgccacaccc
     5221 tgtaatcaca acatgtagtg ctgaattcag ctgctattga cactatatgt tgtgtcatct
     5281 ccctgacctg tgatgcgccg cgcaggggcg gaaaacagcg atatgattat ttcctcagcg
     5341 tggtacactt ccggaaagtt gtgatattca ggaaagtcgg atctgacgaa aacggctctc
     5401 cggtaattta actgagtggt tatgtggctg cttattatca gcattgaaat gatgacgtca
     5461 tgggaaaatc atccgtgtga cagaagcagg gttgtgctgc gcgtcatcat tactctgcgt
     5521 gactattgaa ccggatacta tttttgaggc gaggtatggg taaaccaaca tttagatcat
     5581 tttacgatgt ggtccgggag cttgaggatg tatatggcca taaagagcta tggctgtatt
     5641 caggagcggc atacgcaacg ccaacagaaa tgattaacgc cagacataac tggaaatcgc
     5701 caaaaatcct gaaacgtaac ggaagaatgg ttgcagaacg aatggataac tcggactcat
     5761 ggcaactggt tggtgactac aaaaaaccgc tgttccaaca ttgtgctccg ccctggcaat
     5821 cctgtcagat agatgactat tttaaaggct attacattat cgccccctga aggtcagagg
     5881 agtaattgat ggcgggatta catcattgat ggcgaggttt aaatgggaag tatgaaagaa
     5941 ctgctgtttg aaatgcagga ggaacggcgt gacgaatgga ttgcggaaaa ttatccggat
     6001 gctgaggaag gaactcctga atgggatgcg gcagcgcagg aatacagctg gtttcaggac
     6061 tggatggaag aggcggctga gcagcaatat tttgaggctt cgcttgccag tattcctgac
     6121 cgcctgcagg atgcaaaggc tgaactggat gagttagaga gcctgatgca gttcaaccaa
     6181 cccaggatcg tagagcggat ggcttacgtt cattgcgtga gtgtactgga cagctttctg
     6241 atgtacagcg caagagcact tcttagtcat cccccgcatt tacagaagtt cctgcacgag
     6301 gctgattcgt tggttcctaa taaggaagac agaagaaaat tacttgcttc caaatgggtc
     6361 gagcaggagc cggataaaga cactcctgag aaggtttata cctggcgagc tcagtctctt
     6421 gtagcgaaga agacatttca gagccacaaa gtgataggtc ggtatttttc aaggatgctg
     6481 acaacgccac atgagtggcc attggaggag attaagggtg taatcaagac ccgaaacgca
     6541 ctggtacacc gtaatggagt taccgaatct cttgaacccg tgtatatcag ttcggggagt
     6601 gtgcagaatg ctatctgcac ggtacgagca tttattaccg tggcggctga aacccttctg
     6661 caggaagatg cactatacag gacggatgat ggcatattct gaaattaccg cagcgacacc
     6721 atccataagc gtcttcactg agggatatgt cgatgacttc gcttcccgct aactgtatac
     6781 cagtaagcaa cacatcatta cctgtcgcca tagactaccc agcagcgctg gcacttcgcc
     6841 agatggctct cgttcaggac gaactcccga aatacctgct ggcgccggaa gtcagcgccc
     6901 tgctccacta cgtgccggat cttcaccgca agatgctgct ggcgacgctc tggaacacgg
     6961 gcgcgcgaat taacgaagcg ctggcgctga caagggggga tttttcgctt gcgcctccgt
     7021 atccgtttgt gcagcttgcg accctgaaac aacggacgga aaaagccgcc aggacggcag
     7081 gaagaatgcc cgccggtcag cagactcacc ggctggttcc gctctccgac gcctggttcg
     7141 tcagccagct gcagacgatg gtggccacac tgaaaatccc catggaacgg cgtaataaac
     7201 gaacaggcag gacagagaaa gcgcggatct gggaagtgac ggacagaacg gtcaggacct
     7261 ggattgggca ggcggttgcc gccgctgccg ctgacggtgt gacgttctct gtcccggtca
     7321 cgccacatac gttccgccat tcctatgcga tgcacatgct gtatgccggt ataccgctga
     7381 aggtcctgca gagcctgatg gggcataagt ccatcagctc aacagaggtc tacacgaagg
     7441 tgtttgcgct ggatgtggct gcacggcacc gggtgctgtt ttcgatgcct gagtccgatg
     7501 ctgtcacaat gctgaaaaac agacacgcat aagtcacaaa tcataattat gaattgtgat
     7561 ttattctata aaagaagaga ccactgcaat atgtgatctc ttgtatgcaa gggtgcttaa
     7621 acagtgtgaa ttcacaagtg tgatttcata agtaataact tcttgattat tacgttagtg
     7681 tttttaagta ttgctcaagg gctactctga cgatggcact tacatttttt ggatctttac
     7741 cattgcgttt attcctcaat gccagatctt ctattgtgac aagcatgctt tcaccaagag
     7801 agattgtcgt ccgacattgt ttttctggtt cgggtttttc cggcgcgccg tatggtttat
     7861 ccgcaaggcg ctgagccaga gcctctgctt gttctaccgt aactgcagct ctgtttaatg
     7921 cttgctggct gggtttcttc accatgggcc aaatacctca ttcattaaat gttcaatttc
     7981 tgctctggct gctgtattgt tcgtttctac tacaccggtg ccgttgctca tgcaatcgcg
     8041 atagactttt ctgaaacaaa taactgaatc caggacttga attgttggga attcttcaag
     8101 atattcaagg aattcttttc tctcatttcc cctcaaaacc ggattggttg ttgccatact
     8161 ttgtaaacaa taaaccttaa gttccgggtt aaggttacgc attgcgtcta tctgttgttc
     8221 tagttctgta agagtatcca gatctggttg agaacactgt agtggcgcaa ttatttgatg
     8281 tgcgacaact ccactcgtta tgaattcctt tgagtttctt cctgcgacat ccactattac
     8341 atcatcgaac ttttcattca gagcctgtag tgtttgtgtc aggttatcaa atttctcaac
     8401 aagggttatt gctggtaaca gtcctgcagc ttctcgttct gcatgatgtt ttgcggctgt
     8461 tcgttgtaga tctccgttta acaggcagac ttcccttcct ttatttgcga gagcaatagc
     8521 caggtttgat gcaatggtag ttttccctga accacctttg ttcccaccaa ctacagtaat
     8581 cattgcaacc tcataaatgt gatgtgtgaa gtatgatgat attttgacac ggtaacctga
     8641 tgtgtcaaca tgcattcata tttgtgaagt atgaaatatg aagtatgtca tatgatgtaa
     8701 taaatattac ttgtgttttg tgatttcata actatgatag tgctcaaatt gagtattacc
     8761 ttgcaaccag aaacagtgat ttactcagat tgagtattta aggggctata gatgaacgta
     8821 tactgcgatg atggttcaac aacaatcaag ttggcatgga atgacaatgg gaaaatctgt
     8881 aagtcgttgt cgcaaaactc atttcgtcat ggatggaagg ttgacgggtt agggattcgc
     8941 cagacattta actatgaact ggatggtaaa aaatacacgt atgatgaagt tagcaaccaa
     9001 tctattctta cgactcatat tgagtatcaa tatacggatg ttaacctgtt agctgtgcat
     9061 cacgctttac tgaatagtgg attagcgcct cagcctgtat cattgactgt gactttgcct
     9121 atcagtgagt tctacacgaa agagtgtcaa aaaaatgaac tcaacattca gcgtaaaatt
     9181 gagaatttaa tgaggccgat tcgtcttaat aaaggcgatg ttttcaccat tgaacacgtt
     9241 gatgtgatgc ctgaatcatt gccagctgtg ttttctcgtc tggtcatgga taaagttggt
     9301 cagttcgaaa aatcgctggt agttgatatt ggcggcacta cgcttgatgt tggtgttatc
     9361 gttggtcaat ttgactctgt aagtgccatt catggtaatt ctggcattgg tgtgtcttca
     9421 gttacgaaag ctgcaatgag tgcattgcgc atggcatcca gtgataccag tttcctcgtt
     9481 gcagatgaat tgattaaacg tcgtaatgac ccggactttg ttcggcaggt tataaatgac
     9541 gaaacaaaaa ctgatctggt ccttaatacc attgaggggg ctattgccag tctgggggag
     9601 caagtagtca atgagctcgg tgattttcat catgtgaatc gtgtttatgt tgtcggtggt
     9661 ggggccccgt tgatctatga ttcgataaaa acggcatggc accatcttgg gcaaaaagta
     9721 gtgatgatgg agtcgccaca gacagcactg gttgaagcta tcgcggcttt caaagaggag
     9781 taatattgtg gacgatgaac ggaaaaggaa aaaattcaca ctttatctgc atccggaaaa
     9841 agctgcagac tttcaaactt tggaagcgat agagtccgtt ccgcgttctg agcgtggtga
     9901 gctgttccgg aatgcattta tttctggaat ggcgttacac caacttgatc ctcgcctgcc
     9961 tgtattgctg actgccattt tgagcgagga gttttcagca gatcaggttg taaccttact
    10021 cagtcagacg acaggatgga agccttctca ggccgatatc agagctgtgt tgactgaact
    10081 cggtgctttg caatctgctg aaaaaatgcc tccttctgct actgattcgg tgcaggaggc
    10141 aatgaatgat gtgcgcctta aaatgcaaaa attattctga tatatcagaa tgaagcaatg
    10201 gggatctcag tccattgtgt tgtatacgaa ggcgagagca tttcccgttt catttgccat
    10261 tcaggggcgg ttcctcgccc cgcaaaccac actttccctt ttccagactg gttcagttca
    10321 tcgagtgttt tcattaactt ttcgctgttt ttacggggct ggatctcatc aaataatccc
    10381 ggctgcgcta tacccgatgg tgtgaaatca gccagcatga ctcctgcctt catatagcgg
    10441 tacccttcac gccagacatg gtttaacgct ctgcatgctg cggcaataat gtcccggctg
    10501 tcctgtgtgg gtaatggaag cttttccaca gcggcattgc tgtaacaggg ttcttttact
    10561 gcaaaggggg atgtccgtac aaatgtcgtc acctgccggc aatactgacg ctccccacgt
    10621 agtttctctg cggcccgctc tgcatactga acaacagcct ggtgcatggc atctttgtct
    10681 gtgattcgtt caccaaaact gcgactacag acaatctgct gttttgccgg tggtgcttct
    10741 tccagggata tgcaggactc gccgttgagt tcgcgtaccg tacgctcaag aatgacgctg
    10801 aagtttttcc ggatgaatgc cgtgttagcc tgcgccagct gcagtgctgt gttaataccc
    10861 agcgcattca gcttttccgt cagtctgcgt cctactcccc agacctcacc aactggctgc
    10921 agccccagta gcttcaagat ccgattacgg ttttctgccg tcagcgcgac cacaccggaa
    10981 aactgtggcc attgctttgt tgcccactgt gcacttttag ccagcgtttt tgtaggcgca
    11041 atgcccaccc ccatggtgag tcctgtccag ctttttacct gttccctgag ctgatgacca
    11101 aaaaactccg gagagatgca atgatttatc ccccgcaaat caatgaacat ttcatcaatt
    11161 gagtagggct caactgcggg agaaagcgac tccagaacag ccataacccg ttggctcatg
    11221 ctgtggtaca gcgcataatt gctggaaaat acatgtattt tcttctccag gcgcatctgt
    11281 ctcacctgaa accagggctg ccccattctg atgccaaggg cttttgcctc cgggctgcgc
    11341 gcgatcacac agccatcgtt attgctgagt acgatgactg gttcgttgcg aaggtccggg
    11401 cggaaaactt tttcacatga ggcgtagaaa ctgttgatat cagccagtgc aaacatcagc
    11461 gtaactcccg cgtcctgtgt atcacgtgag tgacaacacc aaaaatacag atgttttccg
    11521 gatacagtgt gcggaattcc gggctgtctg aaaccggctc cagtgccggg cgtgggcgca
    11581 acagcagtcg tttgacggtg aactcaccgt cgatctcagc gataaccatg tccccgtgtt
    11641 gtggtttttc ggccctgtcc actaccagca gatcaccatt ctgcacgcca gcctggttca
    11701 tcgattcacc gctggcgcgc agaaagaagg tggctgcagg tctgctgatg caatagctgt
    11761 tcagatccag ttcctgctca gcataatcag tggcaggcga aggaaaaccg gcctggcaac
    11821 gatcggcaaa caacggtcgc acgtaactgt catcgcctga agggtcagcc ggacggtgat
    11881 aaacggtact catgctgcag gcagccagct gtcgtcctgc cagatttctt ccagcagaga
    11941 catgatacgt tctttatcct tgtcgttgcg ggctcctgtg acagatacag acggggcgct
    12001 gccctggtct atacgcagcc agatttccgg gtagtgtggt ttcagacgtt gttcgatttc
    12061 attctgtagc gcctgaagtg tgcctgattt gaggtttttt gtgctctggc ggtcgaaaag
    12121 aatttcaatg cggatcatgt tctcatctcc cttttctttt actgtatgca tatacagcat
    12181 tctaataact gaatccgcac acagtcaaat gtcattgctc tgactaaaaa atgctcaatt
    12241 ctctgagggt gtggctgtat gcctgctgtg gagtgctgtg tccagcttat ccacagcatt
    12301 ttgtgcacgg ttctgtggac aaaatacctg gttaccctgg ccgtgccggc acgttaactg
    12361 ggctgcatcc gatgcaagtg tgtcgctgtc gccggcctcc tcacccggtc acgtttcgtc
    12421 gttcctcctc cacgcgctgc ggcttcgggg ccgcacctgc attcgtatgc ggtcgcccgg
    12481 ttacaggtgc ggcacggcct gatggaggcc gcatgtgaga ggagaattcc catgccaaac
    12541 tggtgctcga atcgtatgta tttttctggt gaaccagcac agatcgctga gattaaacga
    12601 ctggccagcg gtgcagtcac accgctttat cgccgcgcca cagatgaagg tattcagctg
    12661 tttctggccg gtagtgccgg acttctgcag accactgaag atgtgcggtt tgaaccatgc
    12721 cccggactga cggctgccgg acgtggtgtt gtatcgccgg agaatatcgc gttcacccgc
    12781 tggctgacac acctgcagaa cggtgtgcta ctggatgaac aaaactgcct gatgctgcat
    12841 gaactctggc tgcagagtgg tactggccag cgtcgctggg aaggattacc ggatgaggtc
    12901 agggaaacca tcaccgtgca tttcaccgcc aaaagaggta actggtgtgg cttctggagt
    12961 aacgaggatg tatcggtgtg gtggaaccgt ctgtgtgaca atgtactgcc ggaaaaaacc
    13021 atgccgtttg acctgctgac ggttctgccc acccgcctgg atgttgaagt gaatggcttt
    13081 aacggtggtg ttctgaacgg tgttccttct gcgtatcact ggtatacgga acagtatggc
    13141 gtgaagtggc ctgtggggta tgaggtgaat atcagcagtc agggagacaa cttcattcag
    13201 gttgatttcg acacgccgtg gtgtcagccg gaaagcgacg ttattgcaga attaagccgc
    13261 cgtttcagtt gcacactgga gcactggtat gccgagcagg gctgcaattt ctgtggctgg
    13321 cagcgatatg agcgtggaga gcttgttgat gtcctgtggg gggaactgga atggtcttcc
    13381 ccgacagatg acgatgagct gccggaagtc accggacctg cgtggatagt cgacaatgtg
    13441 gcgcattatg gcggatgaag tatgacggag acgggcgggc aaccgcccgt ttcttttccg
    13501 acaaaggatg tcgccgtgct cctcttttta ctgcgccggc tgacgcggcg cggcaggaac
    13561 gctgcctgtg gtcagtgtcc cgcgtccggc gggcacggga cgggcgcttt tactgctccc
    13621 ggctggcacc ggtgacagtg tacgccagcc tgtcgccctt ttctgatgaa cgcccttcaa
    13681 gccgctttcg cggcatattc tcgccgtcag aaagacgacg gctgcgatat tccacggtcg
    13741 gcctgacccg ttaccggacg cggtgaacag cccacaggca gcggggaacg ggcaccacag
    13801 gggtgccctc ccggtgcctt ctgtaagaga aggagtgttg ttatgtcccg ttttgtcctc
    13861 ggtaactgca tcgatgttat ggcccgtatt cctgataacg ccattgattt catactgact
    13921 gacccgccgt acctcgtcgg tttccgtgac cgttccgggc gcaccattgc cggtgacgtt
    13981 aatgatgact ggctgcaacc tgccagcaat gagatgtacc gcgtactcaa aaaagacgcg
    14041 ctgatggtca gcttctacgg ttggcaccgt attgaccgct ttatggcggc ctggaaacgc
    14101 gccggattca gcgtggtagg gcaccttgtg ttcaccaaaa actacacatc caaatctgcg
    14161 tatgtcgcgt accgccacga atgcgcttac atcctggcaa agggcagtcc ggcccggccc
    14221 cgtaaccctc tgccggatgt gctgggctgg aaatattcgg gcaaccgtca tcacccgacg
    14281 gaaaagcccg tgaccagcct gcaaccactg attgagagct tcacacaccc gaacgcaata
    14341 gtgctggacc cgtttgcagg cagcggctca acctgcgttg ccgccctcca gtccgggcgc
    14401 cggtatatcg gtatcgagct gcttgagcag tatcaccgtg ccggacagca acgactggct
    14461 gccgtacaac gggccatgca gcagggggcc gcgaatgatg actggtttat gccggaggct
    14521 gcataaatga actatgccgg acacgaaaaa cttcgcgccg aagtggcgga ggtggccaat
    14581 gccatgtgcg acctgcgtac aaccatgaat gagatggagc ggcggtacag ctttaatgct
    14641 gacaccctgc cggaacgtct ggtgcgtcag acgttgtttc gcgcaaaccg cctcctgatg
    14701 gaggcatata ccgaaattct ggaactggat gcgtgcttta aagattgaga aggagacgag
    14761 aatgtacgga acatgcgaaa cgctgtgccg ggagctggca gcaaagtatc cgggagagat
    14821 tcaggccctc gctgacggaa tggatatttc cctgtccgat catgaaatca gaaccgtcct
    14881 ggcgcgcctg gaggacatcc cggaagacca gcggactgaa tccggtattt cttccgccgc
    14941 ggtgatggag attatcagca acgtgagcga aaaccgccag gtgaccgtcc ctgctgaact
    15001 gctggcgtcc ctgattcaga ccgctgaaca ggcactgtgg aaacgtgaat gggccgcccg
    15061 tgattacggc ctcgccgtcc cggaatgcgt tacccgccgt caggcggtgg ttaatcaggc
    15121 ccgcaccctg ctgaaaaaca acagacacga aaacgactga tgttatcgcc gcctccgggc
    15181 ggcgattcag ggagaacgat taatcatgaa cgaaaacata acgctgaacg cactgatttg
    15241 tcgtcacgcc cgcaacctgc tgctggcgca gggctggccg gaagagacgg atgttgacca
    15301 gcggaacccg aaccatccgg gctggatcag catttatgtt ctgctggatg cgccccggct
    15361 ggcgacgtta cttatcaacc gtcacggcgg cgcactgccg ccgctcctgg cctccgccat
    15421 tcacaaactg accggaaccg gggcggaact tgtactgtcc ggcagtcagt ggcagtcgct
    15481 gccggtactt ccggcagacg gaacgcaggt gtctttcccg tatgccggtg agtggctggc
    15541 agaggacgaa atcagggcag ttcttgatgc ggtacgcgat gcagtacgct gtgtcagtta
    15601 ccaggtggca gaagatacgc ggcgtatccg ggcggcgctg accaccaccg gtcagacgtt
    15661 actgacccgc cagacgcgcc gctttcgcct ggtcgtgaag gagagcgatc acccctgctg
    15721 gctcgatgag gacgacgaaa acctgcccgt ggtgctcgac gccatcctga accggggcgc
    15781 acgtttttcg gcggtggaaa tgtatctggt cagcgattgt attgaacata tcctgtccag
    15841 ttggctggcc tgcgatgtgc tgcgtatacc ggatgaaccg ccccgccgct ggtttgaccg
    15901 tggtgttctg cgggaagttg tccgggaagc ccgggccgaa atccgcagca tggcggatgc
    15961 cctggcaaaa atccggaaat gatgacgttg gcggggtaaa tccccgcctt tttcctgccg
    16021 gtccccggac agagcacaaa ggaagtcgcc gccggctccg ctttacccgg ccatgcaggg
    16081 catggccttg tggtttttca gttatgtggc ctcagcgtcg tgtgcgggct gtgccgtgcc
    16141 tccatcttag ccgggctggc ggggatgcaa gggtacgctt cgccgctgcg gtcacccggt
    16201 ccctccttcc cgtctgccgt gattttccgg tcgttcgccc gctcaggttt cgcggcctca
    16261 ccctgcaact cccgccagcc gtgtgcgtca ggctgcggct tcccttgcgc cctgtgcatc
    16321 cccgccttat cgcccggctt ttatggaggc acggcaccgc ccggtgtcgc ggaatatgtg
    16381 aacaacggag gaaaacatca tgcaatatgc gaaacctgtc actctgaatg ttgaagagtg
    16441 cgaccgtctc tcctttctgc cttacctgtt tggcctggat tttctgtatg ccgaggcatc
    16501 tgtgtacgcg ctggcgaaaa aaatgatgcc ggaatatgaa ggcggattct ggcacttcat
    16561 ccgcctgccg gacggtggcg gttacatgat gccggatggc gaccgttttc atctggtcaa
    16621 cggtgaaaac tggtttgacc gtacggtcag cgccgatgcc gcaggcatca ttgtgacttc
    16681 ccttgtgatt aaccgtcagt tgtggctgta ccacgacagc ggtgatgcag gactgactca
    16741 cctgtacagg atgcgcgatg cgcagttgtg gagccacatc gaatttcacc ccgaatgtaa
    16801 cgcgatttac gcggcgctgg actgattaac ggacggggcg gcaatgccgc ccctgtaaag
    16861 gagatgagaa catgtactgc actgttaaag aaattatccg cgaagtgctg gatacagacg
    16921 tgccggacag tgaatgcgtt tttgccgtgg tgctgacccg tggggatgtg cgccacatag
    16981 cccaggactg gaacctgtca gacgatgagc tggaaaccgt catgcagcgg ctggacgatg
    17041 cctttgaata tggtgcggat gtcagcattg ttcacgacgt tgttcgtgag ctgatggaag
    17101 aaaagcgtgc cagccgtcag gtgacagttc cggcggtgat gctggaaaaa gtgatggcgc
    17161 tagcaggcag tgaaatgaag cgcctgtatg ccgtcgggag tgagaacggg ggcgacggtg
    17221 atgcgttcgt cagggaggaa cgcgaagcaa tggacgttgt gttacaggcg ctggacgggg
    17281 agcacatgtc atgaatatca gcacagaaac ccgcgaaatt ctgcgcaatt acaaagccgt
    17341 gattaatgcg cgacgtcgtg aaatggggca gaaaccgctc accaccgcac agattgttga
    17401 tgaaatctgc gattttgtgg cgaatcagca ggcggttttc ctgggcggtc actacatctt
    17461 acagggtagc agaaacaggt gatacagggg caggcggtgg aggccgcctg tcagtccgcg
    17521 cgaaaacccc tggcaggctg gcgcctgccg gaagggaagc gggccacgct ggcgcgtcgc
    17581 ccgttaccct tcccgtctgt gatgtgtctg actcaggagt gtttctttct tatgttatgg
    17641 aactgttcct gatgttgttg ttcctttatc tgctgctctg attctgacta ctgcagtgtt
    17701 gttctgtctg ccgggcgcac cttcggctcc cggctgcctg gcggcatccg gtcgtcaggc
    17761 ccgactccga caggcagttg ctggcgcacc tgccagtctc cgccgaaccc gctggcgcgg
    17821 tttcgggctg ccgctacggt ccgatgcgtg aaaaccccgg gcttgcagcg accgttccgg
    17881 tcgcgccgcc tctgtcgcgc cgtggcctgc gctcctggca tcccggcaaa aggccgccgg
    17941 gatgtaaggg gcaacgccgt ttcgcgcacg cgctctccgt cgttgcggcg ggtccctgct
    18001 cattgcctgc cgggtgccac acacccgtat gagtccctgt cgccgggggc tgatacagcc
    18061 acaacctgcc gcactgactg tccagggtaa atgaacagcc cacaaaattt tttacccaaa
    18121 accaggtaaa aaattttgcg ttctgcccag gacaggtgcg tcaggccgtg gcagtgatgc
    18181 cccttgcggc gaaggttctc aaacgggtga agttcatacc acagggaata cagcagcagg
    18241 ttcccgcgaa acgtcaaaac cgcggctccc gaaactcaac gggtgatgtt ctttaaaaat
    18301 caggatttat ttcaggctcc ggcaatacgc atttatttgt tctgtcagtc atccggctgg
    18361 caggtaatga gaacgcagga acagaaggag gaaaacaccg gagaacgtca gacgcagata
    18421 tgaaaaaagc aggtcaggga cctgcttaat ccacgccgta agaattgcgc aaccaatttt
    18481 gacggcattg ttaaccaatt tgtcataagg agtttcattt atgacagtaa gtattgtttc
    18541 accttccgca gcggctgtaa agccccgccg tcatccccgt ttcagacgcg aagatatccc
    18601 cgcgccggaa attgaccccg tgctgaaagc cttcgggcga cacatagccc ggagttttca
    18661 ccgggggcgt ggtgtacaca tccccgccat gaaaaacacg gccttcgggc aggtgctccg
    18721 cacactcgaa ctgaaacgcg cttttaactg atgaggtgag ccgccccgcg aggggcggca
    18781 acagacggaa ggagaacatt ttatgacatt agcagcaatc accatgaccg ccccggaagc
    18841 cgccagccct gtgcagatgt accgcgcgac ctactcaccg gatgacaaca aactgcgcct
    18901 gtatgccgcg tcacgcctcg acccggagac gtataaaaaa gtgcatgatg ccggttttcg
    18961 ctgggcaccc aaacaggcgc tgtttgtcgc gccagcctgg acaccgggcc gggaagacgt
    19021 gctcctctca cttgccggag agattgagga tgaagacagc acgctcgctg aacgtcagga
    19081 agcacgggcg gagcggttta ccggatacag cggaaagcgg gccagtgaat ccgcacaggc
    19141 acttgatgaa gtggaaagac tggccgcgat gatcccgccc ggtcagccca ttcttgtggg
    19201 gcatcacagc gaacgccgcg cccgtcgtga tgcgcagcgt attgaaaacg gcatgaaacg
    19261 tgccgtgatg ctctttgaac gtgcggaata ctgggaagag cgggcgcggt cggcactgct
    19321 tcacgcgaag tataaagaac gtccggacgt tcgctggcgt cgtatcaaaa aaatcgaagc
    19381 tgatttgcgc aaggctgaaa agaccatcgc acagtcgcag aaatatctga cgatgtggcg
    19441 ggctgaatcg ctggatctga atatggcaaa actcatcagc agtcatgacc atatcagcgc
    19501 ctgtttcccg ctggatacgt atccgcgccc ggcagaaaaa agccagtatg aaggaagtcg
    19561 atcgttatgg tcggccctgg atgatgacat catcaccacg gagcaggccc gcgaaattgc
    19621 gatccgctgt catgaacggc agattcagca tcaacaacgc tgggttaacc actatcagaa
    19681 ccgcctgatc tatgagcgtg ccatgctgga cgaaagcggc ggcgtggtta cccggacaca
    19741 ggattttgag ccgggcggac aggttttcag ccggggcgag tggctgacca tcatccgtgt
    19801 gaacaaaagc aacggggcgg tgagttcagt cacaacgcca aattacagtt ttctcgggta
    19861 cagcggcacg atgaaagtga cgcccgatcg catcacggac tacaaagcac catcggcaga
    19921 agaggctgcc gtcgccagtc aggccgcgaa gcgtccgccg gtagtcaact atccggggga
    19981 aggtttccgg gaaatgacaa aggcacagtg ggccgccctg ccccgggact gtaaggccgt
    20041 gcgcagtgtg gcagaagcag aagaccacgg ggcataccgg taccgccgca caatggacaa
    20101 taatttccgc ctggtgaatg tgtatatctc cgacatgaaa atcacggaaa tcccacagaa
    20161 ataaggtata tccccccggg aaaatccccg ggggatgacg gagataatga tatgcacagt
    20221 cagttaagag aacgtatccg gctgatgcgc gcaaggctgg ataacgccgc gccggttgct
    20281 gaaatccggg ctgaatctca gctttttgtg actcctgccc cggtctgtga tcgcctggtg
    20341 atgctggcgg agatcagcaa ccgtgatcac attctggaac cctctgccgg caccggggcc
    20401 atactgcggg cgattcggga taccgcgccg ggggccatgt gtgatgcggt ggaaatcaac
    20461 agcgggctgg tccggtatct gcgggaaaat tttaacggtg tcagggttca gtgtggcgac
    20521 ttcatggaat ggcagccggt gcagtattac agccgggtta tcatgaatcc gccgttcagc
    20581 cacgggcagg atatccggca tatcctgcgg gccttttccc tgttgcgtcc gggtggcgtg
    20641 ctggtcgccg tctgtctcaa cggaccacgc cagcaggaga agctgttacc gttttctgac
    20701 gtccgcgagg agctgccgcg cggcacgttt gcttataccg atgtcccgac gatgattatt
    20761 cgtctgcgtg cctgatgaca cagggccggc gggaggataa atgcctgtct gcccgcgccg
    20821 ggaaaaaacg cgcgccggtg gcgctgaaag gggcggcgcg tgagcgcggc ccctttcatt
    20881 acgcggacgt tctgatgggg gcttctgttt cttcatgcct gtcatttgtc tgtttctgat
    20941 gttgttgttc ctgctactgc tactgctact gctactgcta ctgctactgt tcttcttttt
    21001 cctgctgctc tgattctgac tactgcagtg ttgtcctgtc tgccgggcgc accttcggct
    21061 cccggctgcc tggcggcatc cggtcgtcag gcccgactcc gacaggcagc tgctggcgca
    21121 cctgccagtc tccgccgaac ccgctggcgc ggtttcgggc tgtcgctacg gtccgatgcg
    21181 tgaaaa
//
LOCUS       DAZGOB010000078        21054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGOB010000078 DAZGOB010000000
VERSION     DAZGOB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.078"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..200
                     /locus_tag="ACHLIF_003135"
     CDS             <1..200
                     /locus_tag="ACHLIF_003135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607107.1"
                     /translation="RRPPPCKGGALPAELITPAVWNRIIGRVQNESTHFLKKLFVRRK
                     FKQDDRKTDRVAQFVNENNNA"
     gene            313..1728
                     /gene="gltX"
                     /locus_tag="ACHLIF_003136"
     CDS             313..1728
                     /gene="gltX"
                     /locus_tag="ACHLIF_003136"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO9607108.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1780..2172)
                     /locus_tag="ACHLIF_003137"
     CDS             complement(1780..2172)
                     /locus_tag="ACHLIF_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO9607109.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2174..2533)
                     /locus_tag="ACHLIF_003138"
     CDS             complement(2174..2533)
                     /locus_tag="ACHLIF_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO9607110.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            2754..2829
                     /locus_tag="ACHLIF_003139"
     tRNA            2754..2829
                     /locus_tag="ACHLIF_003139"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2787..2789,aa:Ala,seq:ggc)
     gene            2869..2944
                     /locus_tag="ACHLIF_003140"
     tRNA            2869..2944
                     /locus_tag="ACHLIF_003140"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2902..2904,aa:Ala,seq:ggc)
     gene            3152..5341
                     /locus_tag="ACHLIF_003141"
     CDS             3152..5341
                     /locus_tag="ACHLIF_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO9607111.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGDGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(5391..6593)
                     /gene="nupC"
                     /locus_tag="ACHLIF_003142"
     CDS             complement(5391..6593)
                     /gene="nupC"
                     /locus_tag="ACHLIF_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO9607112.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            6929..8167
                     /locus_tag="ACHLIF_003143"
     CDS             6929..8167
                     /locus_tag="ACHLIF_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO9607113.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(8308..8634)
                     /gene="ypeC"
                     /locus_tag="ACHLIF_003144"
     CDS             complement(8308..8634)
                     /gene="ypeC"
                     /locus_tag="ACHLIF_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO9607114.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(8749..10005)
                     /locus_tag="ACHLIF_003145"
     CDS             complement(8749..10005)
                     /locus_tag="ACHLIF_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO9607115.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            10209..11174
                     /gene="glk"
                     /locus_tag="ACHLIF_003146"
     CDS             10209..11174
                     /gene="glk"
                     /locus_tag="ACHLIF_003146"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO9607116.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11393..11719
                     /locus_tag="ACHLIF_003147"
     CDS             11393..11719
                     /locus_tag="ACHLIF_003147"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO9607117.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            11741..12988
                     /locus_tag="ACHLIF_003148"
     CDS             11741..12988
                     /locus_tag="ACHLIF_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO9607118.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKGLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            13003..14088
                     /gene="ypdF"
                     /locus_tag="ACHLIF_003149"
     CDS             13003..14088
                     /gene="ypdF"
                     /locus_tag="ACHLIF_003149"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9607119.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            14088..15125
                     /gene="ypdE"
                     /locus_tag="ACHLIF_003150"
     CDS             14088..15125
                     /gene="ypdE"
                     /locus_tag="ACHLIF_003150"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9607120.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            15150..17645
                     /gene="ptsP"
                     /locus_tag="ACHLIF_003151"
     CDS             15150..17645
                     /gene="ptsP"
                     /locus_tag="ACHLIF_003151"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO9607121.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILTRASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(17648..18505)
                     /locus_tag="ACHLIF_003152"
     CDS             complement(17648..18505)
                     /locus_tag="ACHLIF_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9607122.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(18518..19252)
                     /gene="ypdB"
                     /locus_tag="ACHLIF_003153"
     CDS             complement(18518..19252)
                     /gene="ypdB"
                     /locus_tag="ACHLIF_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO9607123.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFANKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(19267..20964)
                     /gene="ypdA"
                     /locus_tag="ACHLIF_003154"
     CDS             complement(19267..20964)
                     /gene="ypdA"
                     /locus_tag="ACHLIF_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO9607124.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         4998 a         5257 c         5611 g         5188 t
ORIGIN      
        1 accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccccgctg
       61 tgtggaatcg cattataggg agagttcaaa atgagtcaac gcattttcta aagaaattgt
      121 tcgttcgtcg taaatttaag caagatgatc gcaaaacaga ccgtgttgcg caatttgtca
      181 acgaaaacaa taatgcgtaa ggttgcaacc cgaactacat tgaggaatca ggcgggagtg
      241 atagaatatc gcccactcaa tttttccagg atttgccggt tgtcggcatc tttctaaacg
      301 taaggccatt tcatgaaaat caaaactcgc ttcgcgccaa gcccaacagg ctatctgcac
      361 gttggcggcg cgcgtactgc tctttactcc tggctttttg cacgtaacca cggcggtgag
      421 ttcgtgctgc gtattgaaga caccgatctt gagcgttcca cgccggaagc tatcgaagcc
      481 attatggatg gcatgaactg gctgagcctg gagtgggatg aaggtccgta ctaccagacc
      541 aaacgttttg atcgctacaa tgcggtgatc gatcagatgc tggaagaggg cactgcttat
      601 aaatgctatt gctctaaaga gcgcctggaa gcgctgcgcg aagagcaaat ggcgaaaggt
      661 gagaagccgc gttatgacgg tcgctgccgc cacagccatg agcatcatgc tgatgatgaa
      721 ccgtgtgtcg tgcgttttgc taatccgcag gaaggttctg ttgtttttga cgatcagatc
      781 cgtggtccga tcgagttcag caaccaggag ctggatgatc tgattatccg ccgtaccgat
      841 ggttccccaa cctataactt ctgtgtggtt gtcgatgact gggatatgga aatcacccac
      901 gttatccgtg gcgaagacca tatcaacaac acgccgcgcc agatcaacat tcttaaggcc
      961 ctgaaagcgc cggtgccggt ttacgcgcac gtttctatga tcaatggcga tgacggtaaa
     1021 aaactgtcca aacgtcacgg ggcagtcagc gtaatgcagt atcgtgatga cggttatttg
     1081 ccagaagcac tgctgaacta tctggtgcgt ctgggctggt cccacggcga tcaggaaatc
     1141 ttcactcgtg aagagatgat caaatacttc actttgaatg ccgtcagcaa atctgccagt
     1201 gcgttcaaca ccgacaagct gctgtggctg aaccatcact acattaacgc gctgccgccg
     1261 gagtatgttg ctactcactt acagtggcac attgagcagg aaaatatcga tacccgtaac
     1321 ggcccgcagc tggctgatct ggtgaaactg ctgggcgaac gctgcaagac gctgaaagag
     1381 atggcacaga gctgccgtta tttctacgaa gattttgctg agttcgatgc cgatgcggcg
     1441 aaaaaacatc tgcgtccggt agcgcgtcag ccgctggaag tggttcgtga caaactggcc
     1501 gcgattactg actggaccgc tgaaaacgtt catcacgcta ttcaggcgac ggcggatgag
     1561 ctggaagtgg gtatgggtaa agttggtatg ccgctgcgtg tcgccgtaac cggtgcgggg
     1621 cagtctccag cactggatgt taccgttcac gcaattggta agacccgcag tatcgagcgt
     1681 atcaacaaag cgctggattt tattgctgaa cgcgaaaatc agcagtaatt agcgtgtaaa
     1741 aagataaacg gcaggagata atatcctgcc gttttttatt tatgctgatt cgtcaatatc
     1801 gagacgggcg aggaaattgc gaattccttc acgtgacaaa aattgcgcga ctttttcctg
     1861 ctcaatgtct gtcatattat ctatcgcgtt gatgatttgt cgatacgccg gggcgcgtgc
     1921 tccaggagcg tattcagcaa ttctctcttc agcttccatc aacattggtg tgttatggaa
     1981 ggctggcgtg ttctgaataa actcgcaaac ttgcgtatcg accagaataa gtctggcacg
     2041 accgcctttc acgccgggaa atttttccgt tttccagcct ttttctctga tccaacggtt
     2101 aacagtttgt ttagccacac cgaggcattc agccagttct tcggtggtca ttttgttgcg
     2161 taatcttttc atattatttg ctatcgcgaa tccctaagcg ttgtaacaat ccgataatcc
     2221 cttcccgcag aagcaaggat gtaaactgtt tttgttcaac tggcgtcatc tctttcgcca
     2281 gtgtcaccag caatacttca agcggcgcat caccagaaag ggcaggcgct tctccctgac
     2341 cttctggacg ttcagcattg cgaatatatt cacgaacctg ttcattgacg tgaaccagtc
     2401 gggctttgcc accctggacg ccaggttttg gtgacgttgt ccagccttcc ttgcgtaccc
     2461 atttattaat ggtctggcgg ctatagccgg tcagtctggc aagttcatct ggtgtcatcc
     2521 tctccttgaa cataacaatt ccctgaatgt atgtgggtct attagtagtt cattttataa
     2581 caccacttta caggaaaccg tgacatgaat aactactgaa tgcgagcggt tttccaaaat
     2641 tgttcatttg cctgattttt cagcgattga aactacacgt tgcaatttgc cgttgacaca
     2701 ttcgggcgga attcatatga tgccgcccgt caactcgaca agctttacgt gacggggcta
     2761 tagctcagct gggagagcgc ttgcatggca tgcaagaggt cagcggttcg atcccgctta
     2821 gctccaccaa attttgcacc cagcaaattt ggtacgtaaa cgcatcgtgg ggctatagct
     2881 cagctgggag agcgcttgca tggcatgcaa gaggtcagcg gttcgatccc gcttagctcc
     2941 accaaatttc caaccctcgc tgcaaagcgg gggttttttg tctctgcttt ttgccgcttt
     3001 tgtaatacag tctacgtccg ggttagcgcc gcctggtgaa agcatcattg gatgaaaaat
     3061 cggcaacagg ctggcccctg tttgcttcgc gatgcgaata aacttattat ttgtgtgcct
     3121 gaaaaccccg atcagtgaga gtagtgtact catgtttgtg gagcataacc tgataaaaaa
     3181 tatcaagata ttcacactag cgtttacgct caccgtggta cttattcagc tatcccgttt
     3241 tatttcgcca cttgccatta tccattccag ttatatcttt ctggcgtgga tgccactgtg
     3301 cgtaatgctg tcaatcttgt ttatctttgg ctggcgcggt gtcgttcccg ttttatgcgg
     3361 gatgttttgc accaatctgt ggaactttca tctctctttt ttacagactg cggtcatgct
     3421 cggtagccag acgtttgtcg tgttgtgtgc ctgcgcaata ttacgctggc agctggggac
     3481 gcgttggcgt tatggattga ccagccgata tgtctggcaa cgtctgttct ggcttggttt
     3541 ggtgacgccg atcggcatca aatgcagcat gtatcttgtg gggagcttct ttgatttccc
     3601 gctaaagata tccacctttt tcggcgatgc ggatgccatt ttcacggtcg ttgatttgct
     3661 aagccttttc accgctgtgc tgatttacaa catgcttttc tactatctca ctcgcatgat
     3721 tgtaagtccc cactttgcgc agatattgtg gcgcagggat atcgctccgt cgttgggcaa
     3781 agagaaacgc gcatttacct taagctggct ggcagcgtta agcgtgctgc tacttctgtt
     3841 gtgcacacct tatgaaaacg actttattgc cggttacttg gtacccgttt tcttcatcat
     3901 ctttaccctc ggggtcggta agcttcgcta tccgttttta aatctcacct gggctgtttc
     3961 aacgctttgc ctgctgaatt acaaccagaa ctttttgcaa ggggtggaaa ccgaatattc
     4021 gctggcattt attcttgcgg tgctgatttc ctttagcgtt tgcctgctct atatggtgcg
     4081 catttatcat cgcagtgaat ggcttaatcg ccgctggcat ttgcaggcgc tgacagatcc
     4141 gttaacgctc ctacccaact ttcgtgcgtt ggaacaagcg ccggagcaag aggcgggcaa
     4201 gagtttttgc tgcctgcgca ttgataatct tgagtttatg agtcgtcatt acggcttaat
     4261 gatgcgcgtt cactgtatcc gctcaatttg ccgtacgctg ctgccgttga tgcaggaaaa
     4321 cgaaaagttg tatcaattgc cgggtagtga actgctgtta gtgctgagcg ggccggaaac
     4381 ggaagggcga ctccagcata tggttaacat cctgaatagt cggcaaattc actggaacaa
     4441 taccgggctg gatatgggct atggtgctgc ctgggggcgt tttgatggaa atcaggaaac
     4501 cctgcaaccc ttgttggggc agttaagctg gctggcggag caatcctgcg cacatcatca
     4561 tgtgctggcg ctggatagca gagaggagat ggtttccggg cagaccacta aacaggtgct
     4621 attgctgaat accattcgca cggcgttaga tcagggtgat ttgctgctct acgcccagcc
     4681 aattcgcaac aaagagggtg acggttatga tgagatcctc gcgcgactga aatatgacgg
     4741 cggcattatg accccggata agtttctgcc ccttattgct cagtttaacc ttagcgcgcg
     4801 ttttgatttg caagtgctgg aatccttgtt gaagtggctg gcaacacacc cttgcgacaa
     4861 aaaaggaccg cgcttttcag tcaatttaat gccgctcacg ctgctgcaaa agaatattgc
     4921 cgggcggatt attcgtctgt ttaagcgtta tcacatctcc ccgcaggcgg tcattcttga
     4981 gatcaccgag gagcaggcgt tttctaacgc agaaagcagc atgtacaaca tcgagcagct
     5041 gcataagttt ggtttccgga ttgcgattga tgactttggc accggatatg ccaactacga
     5101 acggttaaag cgtttgcagg ctgatatcat caaaattgat ggcgtctttg tgaaagatat
     5161 tgtcacgaat acgctggatg cgatgattgt gagatcaatt accgatctgg cgaaagcgaa
     5221 gtcattgagt gtggtcgcgg agtttgtcga gacgcaacag cagcaggcgc tattgcataa
     5281 gctcggggtg caatatctgc aagggtattt gattggtcgc ccgcagccat tagctgatta
     5341 acaggcgtaa aaaaaccggg gaattatccc cggctttttt atgtatggtc ttacagcacc
     5401 agtgctgcga ttgacgcaga cagcacactc accagggtag agccgtaaac cagcttcaga
     5461 ccgaagcgag aaaccacgtt accttgctct tcattcaggc ctttaactgc acctgcgata
     5521 atcccgattg aagagaagtt agcgaaggaa accaggaaca cagagatgat gccttcagca
     5581 cgcggagaga gcgtggaagc aattttctgc agatccatca tcgcaacgaa ctcgttggaa
     5641 accagtttgg tcgccatgat actgcccact tgcagtgctt cactggaagg aacacccatc
     5701 acccatgcaa tcggatagaa gatgtagccc aggatgccct ggaaggagat gctgtagcca
     5761 aaccagccag taacggtggc aaacagtgcg ttcagcgcgg cgatcagggc gataaagccg
     5821 atcagcatcg cggcaacgat aatggcaact ttgaaacctg ccagaatgta ttcacccagc
     5881 atttcgaaga agctctgacc ttcgtgcagg ttggacatct ggatgttttc ttcactggca
     5941 tcaacacggt aaggattgat cagcgacagc acgataaagg tgctgaacat gttcagtacc
     6001 agcgcagcaa cgacgtattt cggttccagc atggtcatgt atgcaccaac gatggacatc
     6061 gacacggtgg acattgccgt ggcagccatg gtgtacatac gattacggga gattttgccg
     6121 aggatatctt tataggcaat aaagttttca gactgaccca gaatcaggga gctgacggcg
     6181 ttaaaggatt ccagtttgcc catgccgttg actttggaga gcaggaaacc aattgcgcgg
     6241 atgatcaccg gcaacacgcg aatgtgctgg agaataccga tcagtgcaga gataaagacg
     6301 attgggcaca gcactttcag gaagaagaat gccaggcctt gatcattcat gctaccaaag
     6361 acgaagttag tcccttcgtt ggcaaatccg agcagttttt cgaacatttc ggagaagcct
     6421 ttcacgaagc ctaaaccaac gtcggagttc aggaagaacc acgccagtaa cacttcgata
     6481 acaagcagtt gaataacata acggatacga atttttttgc ggtcgctgct taccagcagt
     6541 gcgagaatcg caacaacggc aagtgccagt acaaaatgaa ggacgcggtc catatttgct
     6601 ccaaatatga ggcaggttaa atttccgtgc acattctatg taacatggtt aaggaaaacg
     6661 agatctgaca cacactataa tgacgtcctg tgacaagctt caaaaatagt gatctgtcat
     6721 acatttctgc tatgttgtgt atggaagctg aaagttatgt aaatgtgcta acattaaaca
     6781 taaccataac attcaagtca tcaatctttt cgtagtcata atcctggtct atcaaagaaa
     6841 tcaccacaac ccattcaaat gaatttgata atcattctcg tttggcatag catgaaacat
     6901 agcaaaggct atgttttaga ggcacaagat gacgaactat cgcgttgaga gtagcagcgg
     6961 acgggcggcg cgcaagatga ggctcgcatt aatgggacct gcgttcattg cggcgattgg
     7021 ttatatcgat cccggtaact ttgcgaccaa tattcaggcg ggtgctagct tcggctatca
     7081 gctactgtgg gttgtcgttt gggccaacct gatggcgatg ctgattcaga tcctctctgc
     7141 caaactaggg attgccaccg gtaaaaatct ggcggagcag attcgtgatc actatccgcg
     7201 tcccgtagtg tggttctatt gggttcaggc agaaattatt gcgatggcaa ccgacctggc
     7261 ggaatttatt ggtgcggcga tcggttttaa actcattctt ggtgtttcgt tgttgcaggg
     7321 cgcggtgctg acggggatcg cgactttcct gattttaatg ctgcaacgtc gcgggcaaaa
     7381 accgctggag aaagtgattg gcgggttact gttgtttgtt gccgcggctt acattgtcga
     7441 gttgattttc tcccagccta acctggcgca gctgggtaaa ggaatggtga tcccgagttt
     7501 acctacttcg gaggcggtct tcctggcagc aggcgtgtta ggggcgacga ttatgccgca
     7561 tgtgatttat ttgcactctt cgctcactca gcatttacat ggcggttcgc gtcaacaacg
     7621 ttattccgcc accaaatggg atgtggctat cgccatgacg attgccggtt ttgtcaatct
     7681 ggcgatgatg gctacagctg cggcggcgtt ccacttttct ggtcatactg gtgttgccga
     7741 tcttgatgag gcttatctga cgctgcaacc gttgttaagc catgctgcgg caacggtctt
     7801 tggattaagc ctggttgctg ccggactgtc ctcaacggtg gtggggacac tggcagggca
     7861 ggtggtgatg caggggttca ttcgcttcca tatcccgctg tgggtgcgtc gtacagtcac
     7921 catgctgccg tcatttattg tcattctgat gggattagat ccgacacgga ttctggttat
     7981 gagtcaggtg ctgttaagtt ttggtatcgc cctggcgctg gttccactgc tgattttcac
     8041 cagtgacagc aagttgatgg gcgatctggt gaacagcaaa cgcgtaaaac agacaggctg
     8101 ggtgattgta gtgctggtcg tggcgctaaa tatctggttg ttggtgggga cggcgctggg
     8161 attgtagttg aatgagcgtc gcatctggca ctattggcgg atgcggcgta aacgccttat
     8221 ccgccctacg cgttaaagag ccggtttgta ggcctgataa gacgcgctag cgtcgcatca
     8281 ggcattgtgc tccattggag gacgcgttta atggcggtgc ccatgtccgc ggccttttcc
     8341 gcgatgatcg tcacgatcgc gccagccttc acgatagcca cgctcatatg ctttacgctt
     8401 atcccagcca cgatgatagc cattatcatg acgccaccag cggtttttgc gccattcata
     8461 attacgatgc cagtaatcac gatctcgcca gtgaccaccg tcccagtaat taccgtaatg
     8521 atcgcgatcg ccaatttgta atttgatcga tggcagtagg gtgatttcac ctgcatttgc
     8581 tgcaagcggg gtaaatgcca tcagggcggc cgccagaaac agtgacctga acattgttat
     8641 tctccttcac gatcgaagcc gtcagcggcc tgttaacgca atattacggg gaggtaaagc
     8701 cccgtatcat cgccataact cttaaatcat aaagaagagc attttttgct aaacattatc
     8761 gtggggtggc tgttgcttcg gacgattgac catcatcatc ggcttacctg ctaataacag
     8821 ccatgccgga agcatgacga tacagagcag cggtagcaat gtggtattgg gtacaacgac
     8881 tgccgccata aaaagactta accagccatc gcgtgttacc accagcacga tgccgagaat
     8941 agcgcaggaa acggttattg ccgctggaac ggcgggaacg tgctcatgca gcatcaaccc
     9001 taatgccacg ccgacaaaca ctgccgggaa gattcgccca ccgcgaaagc cactggcggc
     9061 agcaacgacc agggcggcaa gtttaattac cgccagcaaa aagtaatcgc tggtgctgaa
     9121 agcctgattt gccaccatct gctgcatctc atccagccct ttaaacagcg aaaccggccc
     9181 accaataact cccagaatac cgagaataaa tccgccaata cctagcacga gcaccggatt
     9241 tttcatttga tgcatcatcg cgtgcaaccg tggtaagcac catacggcga ccattcctgc
     9301 tgcgatggca atcgccgcga caattgcacc gctgagaata tcggtcattt ccatctgtcc
     9361 gtaatgcgca atgggcagtg aaaaatgggg atggaaaaat aatccggtag taagtgcacc
     9421 agctgctgcc gccattaatg gcgcaaaaag acgatcccat agcggaactt cactactgcc
     9481 atttaaggtt tgcgaaaata tcaacgccgc cgcaacaggt gtaccaaaca gcgcgccgat
     9541 ggttccggca gaggctaaaa tagtccactc cattcggttg acgcgcggta acagacgagc
     9601 gccaatggca accgcaaggg cgatattgac ggtcatgatc ggatgttccg gccccaggct
     9661 gacgccgcca gcaagaccga gaattaatgc tacgataagt cctggtagcg cagagggcgg
     9721 aactggtgcg ccgatcagcg gttcacaggc ggggtctggt ccggcatgac cctggctgaa
     9781 acggataacc aaccccaccg caatacccgt tagcgttaat acaccgatga tccagagagg
     9841 tgaatcctgg gctatcccca gagttcccgg cagtcgttgc cagagcaaat tctgtaatac
     9901 cgaggcgatt ttcatcacca caatcagaat aagactggac gcaatcccaa ttgccacggc
     9961 ggggagcgat aataacaaca tggttctggc tcgcggatgg agcatgttct attccttatg
    10021 cggggtcaga tacttagttt gcccagcttg caaaaaggca tcgctgcaat tggtgctgaa
    10081 acgataaagt aaatgtgtga cccagatcga tatttacagg gagcctgcct ttccggcgtt
    10141 gttgttatgc ccccaggtat ttacagtgtg agaaagaatt attttgactt tagcggagca
    10201 gttgaagaat gacaaagtat gcattagtcg gtgatgtggg cggcaccaac gcacgtcttg
    10261 ctctgtgtga tattgccagt ggtgaaatct cgcaggctaa gacctattca gggcttgatt
    10321 accccagcct cgaagcggtc attcgcgttt atcttgaaga acataaggtc gaggtgaaag
    10381 acggctgtat tgccatcgct tgcccaatta ccggtgactg ggtggcaatg accaaccata
    10441 cctgggcgtt ctcaattgcc gaaatgaaaa agaatctcgg ttttagccat ctggaaatta
    10501 ttaacgattt taccgctgta tcgatggcga tcccgatgct gaaaaaagag catctgattc
    10561 agtttggtgg cgcagaaccg gtcgaaggta aacctattgc ggtttacggt gccggaacag
    10621 ggcttggggt tgcgcatctg gtccatgtcg ataagcgttg ggtaagcttg ccgggcgaag
    10681 gcggtcacgt tgattttgcg ccgaatagtg aagaagaggc cattatcctc gaaatactgc
    10741 gtgcggaaat tggccatgtt tcggcggaac gcgtgctttc tggccctggg ctggtgaatt
    10801 tgtatcgcgc aattgtgaaa gctgacaacc gcctgccaga aaatctcaag ccaaaagata
    10861 ttaccgaacg tgcgctggct gacagctgca ccgattgccg ccgcgcgttg tcgctgtttt
    10921 gcgtcattat gggccgtttt ggcggcaatc tggcgctcaa tctcgggaca tttggcggcg
    10981 tgtttattgc gggcggtatc gtgccgcgct tccttgagtt cttcaaagcc tctggtttcc
    11041 gtgccgcatt tgaagataaa gggcgcttta aagaatatgt ctatgatatt ccggtgtatc
    11101 tcatcgtcca tgacaatccg ggtcttctcg gttccggtgc acatttacgc cagaccttag
    11161 gtcacattct gtaaatcctt cctgttatat cgggaggtaa ctctcccgat aatcttttaa
    11221 atcatacagt taattcaatt tttctttgtg tcccctcaca agaccgacct gcgtcacact
    11281 tccgtacagc gggattaatt ctccagtaaa tgcattattt gtctggtaac ggcgatttgt
    11341 tttgcacgtt cataatttca ctcgtcaact atcactgaat gaggaattaa cgatgagtaa
    11401 gaaactaatt gccttatgtg cctgcccaat gggcctggct cacactttta tggccgctca
    11461 ggcgctggaa gaagcggcgg tagaagccgg ttatgaagtg aaaattgaaa cccagggcgc
    11521 ggacggtatc cagaatcgcc tgacggcgca ggatatcgcc gaagcaacca tcatcatcca
    11581 ctccgtggca gttaccccgg aagataatga gcgtttcgaa tcacgcgacg tttatgaaat
    11641 cactttgcag gacgcaatta aaaacgctgc gggcatcatc aaagaaatcg aagagatgat
    11701 tgcctctgaa caacagtaat attcgacagg gataattgtt atggccatta aaaaacgcag
    11761 tgcaaccgtt gtgcctggcg catccggtgc ggcagcggca gttaagaatc cgcaggcctc
    11821 taaaagcagc ttctggggtg aactcccgca gcatgtgatg tcagggattt cacgcatggt
    11881 gccgacctta attatgggcg gtgtgatcct cgctttcagc cagctgattg cttatagctg
    11941 gcttaaaatt cctgctgaga tcggcatcat ggatgctctt aatagcggga aattctccgg
    12001 tttcgacctc tccttactga aatttgcctg gctgtcgcag tccttcggcg gcgtgttgtt
    12061 tggttttgcc attccgatgt ttgctgcttt tgtggcgaac tctatcggtg gcaaactggc
    12121 gttcccggct ggttttatcg gcggcttgat gtctacccag ccgacgcaac tgctgaactt
    12181 cgatcccagc actatgcaat gggcgacctc ttcgccggtg ccgtccacct tcattggcgc
    12241 gctgattatt tctatcgttg ccggttacct tgtgaaatgg atgaaccaga aaatccagtt
    12301 accggatttc ctgttagcgt tcaaaaccac atttttgcta ccgattcttt ccgccatttt
    12361 tgtcatgctg gcgatgtact acgtcatcac cccgtttggc ggctggatca acggcggtat
    12421 ccgtactgtg ctgactgccg caggtgagaa gggcgcgctg atgtacgcaa tggggatcgc
    12481 cgcggcgaca gcaatcgacc ttggtggccc gatcaacaaa gccgcaggtt tcgttgcctt
    12541 cagctttacc actgaccacg tattaccggt caccgctcgt tctatcgcta tcgttattcc
    12601 tccgattggt ctgggtctgg cgaccattat tgaccgtcgt ttaaccggca aaggcctgtt
    12661 caacgctcaa ctttatccgc agggtaagac cgcgatgttc cttgccttta tggggattag
    12721 tgagggtgcg attccgtttg cgctggaaag ccccatcacc gccattccgt cgtatatggt
    12781 cggcgcgatt gtcggctcaa ccgccgctgt ctggctgggc gcagtgcaat ggttcccgga
    12841 atccgctatc tgggcatggc cgctggtcac taacctgggc gtctatatgg cggggatcgc
    12901 gctgggggca gtcataactg cgctgatggt ggtgttcctg cgtctgatga tgttccgtaa
    12961 aggcaaattg ttaatcgata gcctgtaaga aggaaaatcc agatgacatt actcgcttcg
    13021 ctgcgcgact ggcttaaggc gcaacaactg gatgcagtgc ttctctcctc acggcagaac
    13081 aaacagccgc atctggggat ctccaccgga tcaggctatg tggttattag ccgtgaaagt
    13141 gcgcacattc tggtagattc gcgctatttc gttgaggtgg aagcccgtgc gcaaggctac
    13201 cagctgcatt tgcttgacgc gacgaacacg cttaccacta tcgtcaatca aatcattgcc
    13261 gatgagcagt tgcaaacgct cggttttgaa ggccagcagg tgagttggga aaccgcgcac
    13321 cgctggaagt ctgaactcaa tgcgaaactg gttagcgcca cgccggatgt gctgcggcaa
    13381 atcaaaacgc cagaggaggt ggagataatc cgcctcgcct gtgggattgc cgatcgcgga
    13441 gcggagcata ttcgccgctt tattcaggcg gggatgagcg agcgcgagat agccgctgaa
    13501 ctggagtggt ttatgcgcca gcagggcgca gaaaaagcct cttttgacac cattgtcgcc
    13561 agcggctggc gcggggcgct gccgcacggc aaagccagcg acaagattgt tgcagcgggc
    13621 gagtttgtca ctctcgattt tggtgcgctg tatcagggct actgctctga tatgacgcgc
    13681 accttgctgg tgaatggcga aggggtgagc gccgaatctc acccgctgtt taacgtctac
    13741 cagattgtcc tgcaggcaca gctcgcagca atctctgcaa ttcgccccgg cgtgcgctgc
    13801 cagcaggttg acgatgccgc gcgccaagtg attaccgagg caggttttag ccactatttc
    13861 ggtcataaca ccggtcatgc tatcggcatt gaagttcatg aagatccgcg tttttcaccg
    13921 cgggacacca cgacgctaca gccaggcatg ttactgaccg tggagccggg gatttatttg
    13981 ccagggcaag ggggcgtgcg catcgaagat gttgtgctgg ttaccccgca aggcgcagaa
    14041 gtgctctacg ccatgccgaa aacagtgttg ctcacgggag aggcataatg gatttatcgc
    14101 tattaaaagc gttgagcgag gcagatgcga tcgcctcctc ggaacaggaa gtgcggcaga
    14161 tcctgctgga agaagcggat cgcctgcaaa aagaagtgcg atttgatggt ctgggatcgg
    14221 tgctgatccg cctcaatgaa tcgacaggtc cgaaggtgat gatctgcgcc catatggacg
    14281 aagtgggatt tatggtgcgt agcatctccc gcgaaggggc aattgatgtg ctgccggttg
    14341 gcaacgtgcg catggctgcc cgccagctgc agccggtgcg catcaccacc cgtgaagagt
    14401 gcaaaattcc aggcctgctt gacggcgacc ggcaggggaa tgacgtcagc gccatgcgcg
    14461 tggacattgg tgcgcgctcg tatgacgaag tgatgcaggc gggaattcgt ccaggcgatc
    14521 gcgtcacctt tgatacaact tttcaggttc tccctcacca gcgggtgatg gggaaagcct
    14581 ttgatgatcg cctcggttgc tatctgctgg tgacgttact acgtgagctg cacgatgccg
    14641 aactgcctgc ggaggtgtgg ctggtggcaa gttccagcga agaggtggga ttacgcggtg
    14701 ggcaaaccgc tacccgcgcg gtgtcgccgg acgtcgccat tgtgctggat accgcctgct
    14761 gggcgaaaaa ctttgattat ggcgcggcta accatcgcca gattggtaac ggcccgatgc
    14821 tggtgttaag cgacaagtca ctgattgcgc cgccaaaact taccgcctgg atcgaaaccg
    14881 tggcggctga aattggcgta ccgttgcagg cagatatgtt cagcaacggc ggcacggacg
    14941 gcggagcggt gcacttaacc ggtactggcg tacccacagt ggtaatgggg cctgccaccc
    15001 gccacggaca ttgcgcggca tcgattgccg attgccgcga cattttgcag atggagcaac
    15061 ttttatctgc ccttattcaa cgtcttacgc gtgagacggt tgttcaactg acggatttca
    15121 gatgatctcc tgattaaccc ggagcggtta tgttaacgat tcaatttctc tgtcctttgc
    15181 ctaacggtct acatgctcgt ccggcgtggg aacttaaaga acagtgcagc cagtggcaaa
    15241 gcgaaatcac ctttttcaac catcgccaga acgcaaaggc cgatgctaaa agctcgctgg
    15301 cgttgattgg aactggcacc ttatttaatg acagttgcag cctgaacatt agcggcagcg
    15361 atgaagagca ggcgcggcgc gtgctggaag agtacatcct ggtgcgcttt atcgacagcg
    15421 acagcgttca gccgacgcag gcagaactga cggcgcatcc gctgccgcgt tcattaagcc
    15481 gccttaaccc ggatttactg tacggcaatg tgctggcaag cggcgtcggc gtgggtacgc
    15541 tgaccctgtt acaaagcgac agcctcgaca gttatcgggc aatccccgcc agtgcgcaag
    15601 attccacccg gctggagcac agcctggcaa cgcttgccga gcaactgaac cagcaattgc
    15661 gtgagcgtga cggcgaaagc aaaactatcc tcagcgccca tttgtcgctg attcaggatg
    15721 atgaattcgc gggcaatatc cgtcgtctga tgacagaaca gcatcaaggg ctgggggcgg
    15781 cgatcatcag caatatggag caggtttgcg ccaaactttc tgcttctgcc agtgattatc
    15841 tgcgcgaacg tgttagcgac attcgcgata ttagcgaaca gttgctgcat atcacctggc
    15901 cggaactgaa gccgcgcaac aatctggtgc tggaaaaacc gactattctg gtagccgagg
    15961 atttaacccc aagccagttt ttgagcctcg atttaaaaaa tcttgcaggc atgattctgg
    16021 agaaaactgg gcgcacctcg cataccctga ttctgacccg cgcctcggcg atcccggtac
    16081 tgagcggcct gccgctggat gcgattgccc gttatgccgg gcaaccggcg gtgcttgacg
    16141 cccagtgcgg cgtgctggcg attaacccga atgacgcggt gagcggttat tatcaggtcg
    16201 cacagacgct ggcggataaa cgccaaaaac aacaggcgca ggccgccgcg cagctggcct
    16261 attcccgtga taacaagcgt attgatattg cggcgaatat cggcaccgct ctggaagcgc
    16321 caggcgcgtt tgccaacggc gcggaaggcg ttggactgtt ccgtactgaa atgctctata
    16381 tggatcgcga cagcgcgccg gacgagcagg agcaatttga agcctaccag caggtgctac
    16441 tggcagcggg cgacaagccg attatcttcc gcacaatgga catcggtggc gataaaagca
    16501 ttccttatct gaatattccc caggaagaga acccgttcct cggctatcgc gcggtacgta
    16561 tttacccgga atttgctggc ctgttccgca ctcaactacg ggcgattttg cgcgccgcca
    16621 gttttggcaa cgcccagttg atgatcccga tggtccacag tctcgatcag atcttatggg
    16681 tgaagggcga gatccaaaaa gcgatcgttg agcttaagcg cgatggcctg cgtcatgcag
    16741 agacgattac gctggggatc atggtggaag tgccgtcggt gtgctacatc atcgatcact
    16801 tctgcgatga ggtcgatttc ttcagtatcg gctccaacga tatgacccag tatctgtatg
    16861 cggtcgatcg taataacccg cgcgtatcgc cgctgtataa cccgattacg ccatcgttcc
    16921 tgcgcatgtt gcagcagatt gttaccactg cgcatcagcg gggcaaatgg gtaggcattt
    16981 gcggtgaact gggcggtgaa agccgttatc tgccgctact gcttgggctg ggcctggatg
    17041 agctgagtat gagtagcccg cgtattccgg cggtgaaaag ccagcttcgt caactggata
    17101 gcgaggcgtg tcgggaactg gcgcgtcagg catgtgaatg ccgcagtgcg caggaaattg
    17161 aagcgttact caccgccttt acgccggaag aagacgttcg cccactgctg gcgctggaga
    17221 atatctttgt tgatcaggat tttagcaata aagagcaggc gatccagttc ctgtgcggca
    17281 acctcggcgt taacgggcgc actgaacatc cgtttgagct ggaagaagat gtctggcagc
    17341 gggaagagat tgtcaccacc ggcgttggtt ttggcgtagc gatcccgcac accaaatctc
    17401 agtggatccg tcattccagt atcagcattg cccggctggc gaaaccggtt gactggcagt
    17461 cagaaatggg cgaagtcgaa ctggtgatca tgctgacgct gggtgctaac gaagggatga
    17521 atcatgtgaa agtcttctcg cagctggcgc gtaaactggt gaataaaaac ttccgccagt
    17581 cgttgtttgc cgcgcaagat gcacaaagta tcctgacgct gctggaaaca gaattaacct
    17641 tctgacgtta gccctgaaaa cgggcgctgt actctcccgg cgtcagacca aactgacgcc
    17701 ggaaaacgcg acaaaaatag tcgctatccg gaaaaccgca acgctgtgcc acttcatgaa
    17761 ttgacagatg atatttctgc aaaatcatcc tcgccttcgc cattcgcacc caacgcacat
    17821 actcgataaa acgcatcgtt ccatgctgag caaacagttt tgacagatga ttgggcgtga
    17881 tattaaaaaa ctgcgccacg ctctcgcggg tgagcggctg ggcataatta tcctgaaccc
    17941 agttacaaat gctgtgatag agaaaatcgc cgcgcggttg cgaattaccc ggcggcatat
    18001 tcaccacgtt gcggcaaaga tgcagcaaac ttaacaccag tggctgaatg atattctgct
    18061 ccagcggcga acggctaaga tgggtgaggg cggtaagcat cgcttcacct tcaccgcgtt
    18121 gcaggctggg aagctggatc tggcgagcag gatgcaacaa cgacgtggtg cggctatcgt
    18181 aaaacgataa cccaagccag gtcggtgcga acaccaggct taacagcatc accggtttgt
    18241 tgttgatcgg taaattagcc gcgcgtgccg gaataaacag catttctcct tccaccattc
    18301 gctgctgctt tgcttccagc aggttgccgt attcgccgcg cagcacgata tccagacggg
    18361 gaaaatcaat acataaactg cccaccggta acgaggcagg ctggctggca aaccagacgc
    18421 gccccagtag ttgcgggtta agcaccaggc cgctgaacag atcggcgaaa aattgttgat
    18481 cggcgggcaa gcctggtgct ttcatgaaag ttcccgatta aagatgcatt aactggcgaa
    18541 attctttcac tttgctgcga ctgaccggca cttcaaaatc cagatctttc agtcgcagaa
    18601 tgtaggtgtt attaaaccac ggttcgattt cgcgtatttt gttcagattg acacaaaatg
    18661 agcgatggca gcggaaaaaa tgcgacggcg gcagtttgct gcaaaattcg gtaatgttca
    18721 tcggcattac gtaggattca cgccgcgtat agacaaaggt cattttctcg tgcgcttcgg
    18781 cgtaatagat atcgttaatt ggcgtgacga ttattcgctc atctttcacc agattaatgg
    18841 tgtcattctc acgcgttacc gtcgcggcag gcgtactgct ggtctgctgt tgttgccagg
    18901 ccgcttccag tttttgcagc atcccggtaa tacgtgactc ctgatacggt ttgagaatgt
    18961 agtcaaacgc ctccagttca aacgcttcta ccgcatgttc tttccacgcg gtgatgaaca
    19021 caataaacgg tttattggcg aactggctga tgttttgcgc cagcaacacg ccatccagcg
    19081 acggaatatt gatatccaga aaaatggcgt cgacgcggtt atgctgcaaa aacttcaaca
    19141 cgtccagacc gtcgtcaaag gtgccgacaa tctccatctg gctgtgctct ttaattagcc
    19201 agctcagttc ctgttgtgcc aggaattcgt cttcaacaat gatgactttc acaatatcac
    19261 tccggctcaa agcaataacg tagcctgtga ggcgactggg gtgcgttggt taggaatgta
    19321 aaacgcaatt tccgtccccg gctccaggcg gcggatatgc agcccctcgc catacaataa
    19381 cttcacgcga tgatggacat tcagcaggcc aattttattg cccggcattt cattcgcttc
    19441 gacccgctca atcacctttg gatcgatgcc gtgcccggta tctcgcaccg caatgcgtac
    19501 ccgatttccg cactctgcaa cgctgatggt gacaacgcct ttacctttgc aaggctgaat
    19561 accgtggaca atggcgttct ccaccaacgg ctggatcagc aggctgggaa tgcagcaatt
    19621 cacctcttca tcaatatcat agataaccgt cagcttgtca ccaaagcggg cctgctcaat
    19681 ggcaatatag tctttaattt gatacagctc ttttttgata tcgatttgct catcgtcttt
    19741 taattcaatg ttatagcgca gataacgcga cagattaaag atcaactggc gagcggtatc
    19801 cggattcaga cggattgacg atgaaatagc gttcagagcg ttaaacagaa aatggggatt
    19861 aattttgctt tgcagggcgc gaagctctgc cttatttgcc atttcacgta gctgctcggc
    19921 gcgtgaaacc tccagttgcg tggagatgat ttgcgacaga ccgaccgcca tttcctgtaa
    19981 tgacgaggtg atctgatgcg cgtggcagta gtaaattttc agcgttccgg tcacgacccc
    20041 tttctcccac aatgggatca ccagcatgga atgaatctct ggtgtgcggt gggcttcatc
    20101 attgttttta atgatgattt ttccgtaatt catcgcctga cgggtagtcg ggctaatgaa
    20161 gtcatcgcca ttctgatagt tatgttcacc cacgccaaca taggccagca catgatcggt
    20221 attagtaatc gccaccgcat cggcgtgaat gtcatcgcgg ataatttcgc agaccttgcg
    20281 taatgactcg ctattgacat ggcgaaacag cggtagcgtt ttgttggcga tatccagcgc
    20341 cagcttggcc tgccgcgcgg cgctggcctc tttttcgccc tcaacgcttt gcaccagaag
    20401 cacaataaag ccgatacaga cgctaccgag gatcattgga atgccgattt tagagacgat
    20461 atcgatcccc aacgcggtag ttggtgccca gacaattacc agaatcatgg tcaacgtctc
    20521 acacaacatg ccgccgagaa taccgacgcg ccagcgctgt gctttgggga ttttcaggtt
    20581 gatccagccc gatatacaac ccgccagaat gctggtgata aagcagggga tcgccgtcac
    20641 gccgccgata tcaattaaat accggtgaat acccgcaatc acgccggtaa tgatgcctac
    20701 ccacgggcca aacagaatcc cgccggacat caccgcgata atacgtacgt tcaccagcga
    20761 gccttctacg ggaacgccgg accaggtgct gaacagggcg aacagcgaga aaatggcggt
    20821 aacggcgagc aattcttttg gggagtgcgc cgacttgtgc aacagttcgc gaaacaggcg
    20881 gatacggatg agaaagaaca ggcagataag cattaacgct gcccgatcga agaccgccag
    20941 cagcatgttg aatatttcgt gcacgggtga ctctgaataa ggattaaacc tctatgatag
    21001 gtaacctgaa ggctgatgac cagcaggccg tttttgagga aaaaccataa attt
//
LOCUS       DAZGOB010000079        20995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGOB010000079 DAZGOB010000000
VERSION     DAZGOB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.079"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..127)
                     /locus_tag="ACHLIF_003155"
     CDS             complement(<1..127)
                     /locus_tag="ACHLIF_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607125.1"
                     /translation="MRDNGLNNMFFSLCQINDNHSFTSSSHTKKTKSYNYSKHHKN"
     gene            complement(305..1954)
                     /gene="actP"
                     /locus_tag="ACHLIF_003156"
     CDS             complement(305..1954)
                     /gene="actP"
                     /locus_tag="ACHLIF_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO9607126.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1951..2265)
                     /gene="yjcH"
                     /locus_tag="ACHLIF_003157"
     CDS             complement(1951..2265)
                     /gene="yjcH"
                     /locus_tag="ACHLIF_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO9607127.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2466..4424)
                     /gene="acs"
                     /locus_tag="ACHLIF_003158"
     CDS             complement(2466..4424)
                     /gene="acs"
                     /locus_tag="ACHLIF_003158"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO9607128.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4816..6252
                     /gene="nrfA"
                     /locus_tag="ACHLIF_003159"
     CDS             4816..6252
                     /gene="nrfA"
                     /locus_tag="ACHLIF_003159"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO9607129.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6297..6863
                     /gene="nrfB"
                     /locus_tag="ACHLIF_003160"
     CDS             6297..6863
                     /gene="nrfB"
                     /locus_tag="ACHLIF_003160"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO9607130.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6860..7531
                     /gene="nrfC"
                     /locus_tag="ACHLIF_003161"
     CDS             6860..7531
                     /gene="nrfC"
                     /locus_tag="ACHLIF_003161"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO9607131.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7528..8484
                     /gene="nrfD"
                     /locus_tag="ACHLIF_003162"
     CDS             7528..8484
                     /gene="nrfD"
                     /locus_tag="ACHLIF_003162"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO9607132.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8504..10222
                     /gene="nrfE"
                     /locus_tag="ACHLIF_003163"
     CDS             8504..10222
                     /gene="nrfE"
                     /locus_tag="ACHLIF_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO9607133.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10215..10598
                     /gene="nrfF"
                     /locus_tag="ACHLIF_003164"
     CDS             10215..10598
                     /gene="nrfF"
                     /locus_tag="ACHLIF_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO9607134.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10595..11191
                     /gene="nrfG"
                     /locus_tag="ACHLIF_003165"
     CDS             10595..11191
                     /gene="nrfG"
                     /locus_tag="ACHLIF_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO9607135.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11533..12846
                     /gene="gltP"
                     /locus_tag="ACHLIF_003166"
     CDS             11533..12846
                     /gene="gltP"
                     /locus_tag="ACHLIF_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO9607136.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12924..13613)
                     /gene="yjcO"
                     /locus_tag="ACHLIF_003167"
     CDS             complement(12924..13613)
                     /gene="yjcO"
                     /locus_tag="ACHLIF_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO9607137.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13707..15854)
                     /gene="fdhF"
                     /locus_tag="ACHLIF_003168"
     CDS             complement(13707..15854)
                     /gene="fdhF"
                     /locus_tag="ACHLIF_003168"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15435..15437),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO9607138.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16052..17518)
                     /gene="mdtP"
                     /locus_tag="ACHLIF_003169"
     CDS             complement(16052..17518)
                     /gene="mdtP"
                     /locus_tag="ACHLIF_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO9607139.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALLQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVALFAGLDLDLWGV
                     HRSAVAAAIGAHNATLAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFQGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17515..19566)
                     /gene="mdtO"
                     /locus_tag="ACHLIF_003170"
     CDS             complement(17515..19566)
                     /gene="mdtO"
                     /locus_tag="ACHLIF_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO9607140.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLFIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19566..20597)
                     /gene="mdtN"
                     /locus_tag="ACHLIF_003171"
     CDS             complement(19566..20597)
                     /gene="mdtN"
                     /locus_tag="ACHLIF_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO9607141.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20616..20891)
                     /gene="ytcA"
                     /locus_tag="ACHLIF_003172"
     CDS             complement(20616..20891)
                     /gene="ytcA"
                     /locus_tag="ACHLIF_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO9607142.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4753 a         5534 c         5732 g         4976 t
ORIGIN      
        1 tgtttttatg atgtttgctg taattatatg attttgtttt ctttgtatgt gatgaacttg
       61 tgaaagagtg gttatcgtta atctggcaaa gagagaaaaa catattattt aaaccattat
      121 cgcgcataaa atatccttat aattaacaag aaagaaaagg catattctct gcattatcat
      181 tttctacgac tgtcaaaaat cgctcatttt ttaatgagtt tatttgttta atattatggg
      241 aaaaggtgat gcatgctgga gaggaagagt attccccggt caggcgaccg gggaagggga
      301 gagattaatg tgcgcggcct tgctcaacgc caaagccggt ctgggagcgg ataaactgtg
      361 cacggaacag ttcacgctcg cgcgcacctt ccgctgagtt atcggttgcc gagaagaacc
      421 agatgccgag gaatgccacg ctgatagaga acagcgccgg gtattcatac gggaagatgg
      481 ctttttcgtg accaaggatc tgtacccaaa tcgtcgggcc gaggatcatc agtaccactg
      541 cggtaatcag ccccagccag ccacccatca tcgcgccacg agtggtcagt ttcgaccagt
      601 acatagaaag cagaatgatc gggaagttac agctcgctgc gatggcaaac gccaggccta
      661 ccatgaaggc gatgttctga ttctcaaaca gcacgccgag gataatggca atcacaccta
      721 aaatcagcac ggtgattttc gatacccgca gttcttcacg ttcggtcgcg ccttttttga
      781 agacgttagc gtacaggtca tgcgaaaccg ctgatgcgcc cgccagcgtc agaccagcaa
      841 ccaccgcaag aatggtggca aaagcaaccg ctgaaataaa accgaggaac aggttgccgc
      901 ccactgcatt cgccaggtga acggccgcca tgttgttacc accaatcaga tggcccgccg
      961 cgtctttata ttccggattc gcaccaacca gcatgatcgc gccgaagccg ataataaagg
     1021 tcagaatata gaagtagccc ataaacccgg tggcgtagaa cacgctctta cgtgcttcgc
     1081 gggcatcgct gactgtaaag aagcgcatca gaatgtgcgg caagcccgcc gtaccaaaca
     1141 tcagtcccag acccagagag agcgcggaga tcggatcttt caccagcccg cccggcttca
     1201 tgatgtcgac acctttcggg tgtaccgcca tcgcttcact gaacagattg ttgaagctaa
     1261 agccgacgtg tttcatcacc ataaaggcca taaagctggc accgaacagc aacagcacgg
     1321 ctttgataat ctgtacccaa gtggtcgcca gcatgccgcc gaacaggacg tacatcatca
     1381 tcagcacgcc gaccagcacc accgcaatgt gatagttaag gccaaacagc agctcgatca
     1441 gtttacctgc gcccaccatc tgggcgataa ggtaaagcgc caccaccacc agagaaccac
     1501 aggccgaaag aatacgaatc ggcccttgtt tcagacggta agaggccaca tcggcaaagg
     1561 tgtagcgccc caggttacgc agacgttcgg caatcaggaa caaaatgatc ggccagccca
     1621 ccaggaagcc cagcgagtaa attaagccgt catagccgga ggtaaacacc agcgcggaga
     1681 tccccaagaa tgaggcggcg gacatatagt ccccggcaat cgccagcccg ttctggaagc
     1741 cagtgatatt gccgcctgcg gtgtagtagt cgctacgaga acgtacgcgt tttgatgccc
     1801 agtaggtaat gccgagcgta aacacgacga aaatcaggaa cataataatc gcctgccagt
     1861 tcgttggctg gcgctctacg gccccgctaa tagcatccgc ggcgttagct gcgaaaggga
     1921 gtgtggcggc aagcgccgtc agaactcttt tcatgatgct tgtacctcat gcaggacttc
     1981 attattaaga cggtcgaatt cgccgttcgc ccgccagatg tagataccgg tgagaacaaa
     2041 ggagatcaca atcactccaa caccaattgg aataccgcgt gtgacgctgg tgttcggatt
     2101 cagcggcgtg cccagccagc cgggcgcgaa ggcgatcagt aaaataaagc cgatataaac
     2161 tgccagcata ataatcgaca ggatggtggc aaaccgttgc cgtttttcga ctaactccct
     2221 gaaatgcgca ttgtcttcta tccgctgata aatcgtacca ttcatcacag attctccaga
     2281 ggtaatgtag ggattgtttt aattcccgct cccttatggg agaaggttaa cgctcgggta
     2341 acccttgccg aatgtaggcc ggataaggcg ttatacgccg catccggcaa tcaatgcctg
     2401 atgcgacgct gtcgcgtctt atcaggccta caaaccgtta ccgactcgca tccggcaatt
     2461 gtgggttacg atggcatcgc gatagcctgc ttctcttcaa gcagcttctc gactacgcca
     2521 ggatcggcaa gcgtcgaggt atcgcccagg ttgctggtat cgcccgccgc aattttgcgc
     2581 agaatacggc gcataatttt gccggagcgg gttttaggca gggagtcggt ccagtgcagc
     2641 acgtctggcg tcgccagcgg gccaatctct ttacgcaccc agttgcggac ttctgcgtac
     2701 agttctggtg acggttcctc cccgtgatta agcgtgacgt aggcgtagat cgcctgacct
     2761 ttaatattgt gcggaatacc tactacggcg gcttcggcaa tcttcggatg cgccaccagc
     2821 gccgactcaa tctctgccgt ccccagacgg tgaccggaga cgttcagcac gtcgtccaca
     2881 cgcccggtga tccagtaata gccatcttca tcgcgacgcg cgccgtcgcc gctgaaatac
     2941 atatttttga aggtggagaa gtaggtctgt tcaaaacgtt cgtgatcgcc aaacagcgta
     3001 cgcgcctgac ccggccagga gtcggtgatc accaggctgc cttcggtagc gccttccagc
     3061 gggttacctt cgttatcgac cagcgccggt tgcacgccga agaacggacg tgttgccgaa
     3121 ccggctttca gctcggtagc gccaggcagc ggcgtgatca tgaaaccgcc agtttcggtc
     3181 tgccaccagg tatcgaccac cggacatttc tcgttgccga tttttttcca gtaccactcc
     3241 cacgcttccg ggttaattgg ctcgcccacg gaaccgagaa tgcgcagcga cgaacggtcg
     3301 gtaccctcga tcgctttatc gccttccgcc atcagcgcgc ggatcgccgt gggtgcggta
     3361 tagagaatat tgacctgatg cttgtccacc acctgcgcca tacgggcagg cgtcggccag
     3421 ttgggtacgc cttcaaacat cagcgtggtc gcaccgcagg ccagcgggcc gtacagcaag
     3481 taactgtgtc cggtcaccca gcccacatcg gcggtgcacc agtagatatc acccggatga
     3541 taatcaaaga catatttaaa ggtcagcgcc gcgtacacca gataaccgcc ggtagtatgc
     3601 agcacacctt ttggcttacc ggtagaaccg gaggtgtaga gaataaacag cggatcttcg
     3661 gcgttcatct cttccgcctg gtgctgatcg ctcgcttgct caaccaggtc gtgccaccac
     3721 aggtcgcgcc cttcctgcca gtcaattttc ccgccagtac gcttcagtac caccacatgc
     3781 tctacgctgg tgacgttcgg gtttttcagc gcgtcatcaa cgtttttctt cagcggaata
     3841 ctgcgcccgg cacgcacacc ttcgtcggaa gtgatcacca gtcgtgagtt ggaatcaata
     3901 atgcgcccgg caacggcttc cggcgagaag ccgccgaaaa tcaccgaatg caccgcgcca
     3961 atgcgggcgc aggccagcat cgcaaccgcg gcttccggca ccatcggcat ataaatcgcc
     4021 accacatcac cttttttaat gcccagctcg agcagggtat tggcgaagcg gcacacgtcg
     4081 cggtgcagct ctttatagct gatatgtttg ctctggctgg cgtcgtcgcc ttcccagatg
     4141 atggcggtgc gatcgccgtt ttcttgcaga tggcggtcaa ggcagtttgc cgccagattc
     4201 agcgtgccgt cctcgtacca tttaatggac acattaccgg gggcaaagga ggtatttttc
     4261 accttctggt acggtttgat ccagtcgaga atttttccct gttcgcccca gaaggtatca
     4321 ggtacgttaa tagattgttg atacatcgcc tcgtactgct gagggtttat caggcaacgg
     4381 tctgcgatgt tggcaggaat ggtgtgtttg tgaatttggc tcatgctttt gttctccttg
     4441 taggatgtta ataatatgtg gcataagcgt taaatgtagg ggtattggca gttttgttta
     4501 gtatttgggc gacagatcac gcaaaagtag aattgtgcaa ataaacggca gggtaatttt
     4561 tgaaggtcac caacaaaagt tgattaattc tttgaggaac atgcagttat gcatgctgtt
     4621 gaaaaagagg aagatactga ctaactctaa agtggtattt tacatgcact tacaattgat
     4681 taaagacaac attcacagtg tggttatatg ttacacatag gggcgagcaa tgtcatgaca
     4741 gtgtaggtgc ggttactgtc gtgaaaacaa taaaaaccgc cattgcaaca atggcgcaat
     4801 tcggatgaag cccctatgac aaggataaaa ataaacgcac gccgtatctt cagcttattg
     4861 attccttttt tctttttcac ttctgttcac gctgaacaaa cggcagctcc cgcaaaacct
     4921 gtaactgtgg aagcgaagaa tgaaaccttt gccccgcagc atcccgatca atatctctcc
     4981 tggaaagcca cctcggaaca gtcagagcgt gttgacgccc tggcggaaga tccacggctg
     5041 gtgatcctgt gggcggggta tcccttctcg cgcgattaca acaagccgcg tggacatgcc
     5101 tttgctgtga ccgatgtgcg tgaaaccctg cgtaccggtg cgccgaaaaa cgctgaagat
     5161 ggtccgctac cgatggcgtg ctggagttgt aaaagcccgg atgtggcgcg tctgatccag
     5221 aaagacggcg aagatggcta cttccacggt aagtgggcgc gcggcggccc ggaaatcgtc
     5281 aacaacttag gttgtgccga ctgccataac accgcctcac cagagttcgc caaaggcaaa
     5341 ccggagttaa ccctttcccg tccgtatgcg gctcgcgcga tggaagccat tggtaaacct
     5401 tttgagaaag ccggacgttt cgaccagcaa tcgatggttt gcggtcagtg ccatgtggag
     5461 tattacttcg acggcaaaaa caaagcggtt aaattcccgt gggatgacgg catgaaagtc
     5521 gaaaatatgg agcagtatta cgacaaaatt gccttctctg actggactaa ctccctgtcg
     5581 aaaacgccaa tgctgaaagc gcagcacccg gaatatgaaa cctggacagc gggcattcac
     5641 ggtaaaaaca acgtgacctg tatcgactgc catatgccaa aagtgcagaa cgccgaaggc
     5701 aaactctaca ccgaccataa aattggtaat ccgtttgata acttcgccca gacttgtgcg
     5761 aactgccata cccaggacaa agctgctttg caaaaagtgg tcgcggaacg taagcagtcg
     5821 attaacgacc tgaaaatcaa ggttgaagat caactggttc acgctcactt cgaagcgaaa
     5881 gcagcgctgg atgcaggcgc gacggaagcc gaaatgaagc cgattcagga cgatatccgt
     5941 catgcccagt ggcgttggga tctggcgatc gcttcccacg gtattcatat gcacgcaccg
     6001 gaagaaggtc tacggatgct cggtacggcg atggataaag cggcggatgc acgcaccaaa
     6061 ctggcgcgcc tgctggcgac caaaggcatc acccatgaaa tccagatccc ggatatctcg
     6121 accaaagaga aagcccagca ggccattggc ctgaacatgg aacaaatcaa ggccgagaag
     6181 caggacttca tcaaaacggt gatcccgcag tgggaagaac aggcacgtaa aaatggtctg
     6241 ttaagccaat aaccccgttc cgcctcgcaa ggggcggaaa acacaatgga gtgaatatga
     6301 gcgtattacg ttcgttatta actgccgggg tgctggcgtc gggcttgttg tggagcctga
     6361 acggaattac cgctacccct gcggcgcagg catctgatga tcgctacgaa gttacccagc
     6421 agcgtaaccc ggatgccgcc tgtctggact gtcataaacc agataccgaa ggtatgcatg
     6481 gaaaacatgc ttccgttatc aacccgaata acaaactgcc ggtcacctgc accaactgtc
     6541 acggccagcc atcaccgcaa caccgcgaag gggtgaaaga tgtgatgcgc tttaacgagc
     6601 cgatgtacaa ggttggagag cagaacagcg tctgtatgtc ctgtcatctg ccggaacagt
     6661 tgcaaaaagc gttctggccg cacgatgtcc acgtaaccaa agtggcgtgc gccagctgcc
     6721 attccctgca tccacagcaa gatacgatgc agacgttaag tgacaaagga cggattaaga
     6781 tttgcgtcga ttgccacagc gatcagcgca ccaatccgaa ctttaacccg gcgtctgttc
     6841 cgttgcttaa ggagcagcca tgacctggtc tcgtcgccag tttctcaccg gcgtcggcgt
     6901 gctggcagcc gtcagcggca ccgcagggcg cgtggtggcg aaaacgttga atatcaatgg
     6961 ggtgcgttac ggcatggtgc atgacgagtc gttatgcatc ggctgtaccg cctgtatgga
     7021 tgcttgtcgg gaagtgaaca aggtgccgga aggcgtctcg cgcctgacga ttattcgtag
     7081 cgagccgcag ggcgaatttc ctgatgtgaa atatcgcttc ttccgtaagt cttgccagca
     7141 ctgcgatcat gcgccgtgcg ttgacgtctg cccgaccggt gcgtcttttc gtgatgccgc
     7201 tagcgggatt gtcgatgtta atcctgatct ctgcgtcggt tgtcagtact gcatcgccgc
     7261 ctgtccgtac cgcgtgcgct ttatccatcc ggtcacgaag acggcggaca aatgcgattt
     7321 ctgccgtaaa accaatttgc aggccggtaa gctgcccgcg tgcgttgagg cttgcccgac
     7381 gaaggcgctg acgtttggca atctggacga tcctaacagt gagatttcgc aactgctgcg
     7441 ccagaagcct acttaccgct acaagctggc gctgggaacc aaaccgaagc tgtaccgcgt
     7501 accgtttaaa tacggggagg tgagccaatg acgcagactt ccgcatttca ttttgaatcg
     7561 ctggtgtggg actggccgat tgccatctac ctgtttttga ttggtatttc tgccggtctg
     7621 gtgacgctgg ccgtgctgtt acgtcgcttc tacccgcagg cgggcggtgc agacagtacg
     7681 ttgctgcgca ccacgctgat tgtcgggccg ggcgcggtga tcctcggtct gttgatcctc
     7741 gtcttccacc tgacaagacc gtggaccttc tggaagctga tgttccacta cagttttacc
     7801 tcggtgatgt cgatgggggt gatgctgttt cagctctaca tggtggtgct ggtgctgtgg
     7861 ctggcgaaaa tctttgaaca tgatttgctt gccctgcaac aacgctggtt gccgaagctg
     7921 gggatcgtgc aaaaggttct gagcctgctg acgcccgttc atcgcggact ggaaacattg
     7981 atgctggtgt tggcggtgtt gttgggggct tataccggct ttctgctgtc ggcgctgaaa
     8041 tcgtatccgt tcctcaataa cccgatcctg ccggtgctgt tcctcttctc cggcatctcg
     8101 tccggtgcgg cggtggcgct gatcgccatg gcgatacgcc aacgcagtaa cccgcattcc
     8161 acggaagcgc agtttgtaca ccgtatggaa atccccgtgg tatggggtga aatcttcctg
     8221 ctggtggcat tttttgtcgg tctggcgctg ggcgatgacg gtaaagtgcg tgcgctggtg
     8281 gcggcattag gtggcggttt ctggacgtgg tggttctggc ttggtgtcgc cgggctgggg
     8341 ctgattgtgc caatgttgct caaaccgtgg gtcaatcgca gttccggcat tcctgccgtg
     8401 ctggcggcgt gtggggccag tctggtcggc gtgttgatgc tgcgcttttt cattctctac
     8461 gccggacagt tgacggtggc gtaagccaga aaagaggtgg tttctggacg tattccttcc
     8521 tgaagtcggt tttctggcgt tgttgttaag tctcggggtc aacgtgttga ccccgttgac
     8581 ggcctttgcg ggagtgcggt tgcgctggcc tgccatgatg cgactcactt gcatcggcat
     8641 tctggcgcag ttcgcgctcc tgctgctcgc ctttggcgta ctgacgtatt gttttctcat
     8701 cagcgatttc tcggtcattt atgtcgcgca acatagctat agcctgctgt cgtgggaact
     8761 caagctggca gcggtgtggg gcggccatga aggttcgctg ctgctttggg tgctgctgct
     8821 ttccgcctgg agcgcgctgt ttgcctggca ttatcggcag caaaccgatc cgctatttcc
     8881 gctgacgtta gccgttttat ctctcatgct cgccgcactg ctactgtttg tggtgctgtg
     8941 gtccgatccc ttcgtgcgga tatttccacc agcaatcgaa ggccgcgatc tcaatccgat
     9001 gctgcaacat cccggtctta tctttcatcc accgctgctt taccttggct atggcggttt
     9061 gatggtagcg gcgagcgtgg cgctggcgag tttactgcgc ggcgagtttg atggtgcctg
     9121 cgccagaatt tgttggcgct gggcactacc tggctggagc gcattaacgg cggggatcat
     9181 cctcggttcc tggtgggcct attgcgaact gggctggggc ggctggtggt tctgggatcc
     9241 ggtggaaaac gcctctttat taccctggct ttctgccact gcgctgctgc acagtttgtc
     9301 cctgacacgc cagcgggaga ttttccgcca ctggtcgctg ttgctggcga tagtaactct
     9361 gatgctgtcg ctactgggta ccttaattgt ccgttctggc attctggttt cggttcatgc
     9421 gttcgcactg gataacgtcc gcgccgtgcc gttgttcagc ctgtttgcac tgattagcct
     9481 tgcgtctctg gctctgtatg gctggcgagc gcgggacggt ggtgcggtgg tgcgtttttc
     9541 ggggttatcg cgggaaatgt taatcctcgc tacgctgttg ctgttttgcg cagtgctact
     9601 gatcgtgctg gtgggaacgc tttatccgat gatttacggc ctgctgggct ggggacgcct
     9661 ctccgttggc gcgccgtatt ttaaccgcgc gacgttaccg tttggcctgt tgatgctggt
     9721 ggtgattgtg ctggcgacgt ttgtctctgg caaacgcgtg cagcttccgg cgctggtagc
     9781 tcatgcgggc gtgctgttat ttgccgcggg gatcgtggtt tccagcgtca gccgtcagga
     9841 gatcagcctg aatttacagc cgggtcagca ggtgacgctg gcaggataca ccttccgttt
     9901 tgagcgcctc gatctgcaag ccaaaggcaa ttacaccagc gaaaaagcga tagtggcact
     9961 gtttgaccat cagcaacgca ttggtgaact gatgccggag cggcgttttt acgaagcacg
    10021 tcgtcagcaa atgatggaac cgtcaattcg ctggaacggc atccatgact ggtatgcggt
    10081 catgggtgaa aaaaccggag cggatcgtta cgcttttcgc ttgtatgtac aaagcggtgt
    10141 gcgctggatc tgggggggag gattgttgat gattgcgggc gcattgttaa gcggatggcg
    10201 ggggaggaag cgcgatgaat aaaggacttc tcacgctact gctgttattt acctgttttg
    10261 cgcgcgctca ggtcgtagac acctggcaat tcgccaatcc gcaacaacag caacaggcgt
    10321 taaatattgc cagccagtta cgttgtccgc agtgccagaa tcaaaactta ctggaatcca
    10381 acgcgccggt ggcagtcagt atgcgccatc aggtttacag catggtggcg gaggggaaga
    10441 acgaagtcga aatcattggc tggatgaccg aacgctacgg agattttgtt cgctataacc
    10501 caccgttaac gggtcagacg ctggtgttgt gggcgctgcc agtggtgttg ttactgctga
    10561 tggcactgat cctctggcga gtgagggcaa agcgatgaaa gagcccaaaa taccggtgaa
    10621 aatgctgaca acactcacga ttttgatggt atttctctgt gtcggcagtt atctgttaag
    10681 tccaaaatgg caggcggtac atgcggaata tcagcgtcag cgcgatccgc tacatcagtt
    10741 tgccagccag caaaccccgg aagcgcagct tcaggcattg caggataaaa tccgcgctaa
    10801 tccacaaaac agcgaacaat gggcgttact gggcgagtac tatctgtggc aaaacgatta
    10861 cagcaattcg ctgctggcgt accgtcaggc gttgcaacta cgtggtgaga acgctgaact
    10921 gtatgcggcg ctggcgacgg tgctttatta ccaggccagc cagcatatga ccgcccagac
    10981 tcgcgcaatg atcgacaaag ccctcgcgct ggactctaac gaaatcaccg ctctgatgct
    11041 gctggcttcc gatgcgttta tgcaggcgaa ttacgcgcaa gccatcgaac tatggcaaaa
    11101 agtgatggat ctcaactcac cccggatcaa ccggacacag ctggtcgagt cgattaatat
    11161 ggcgaaattg ttgcagcgga gatcagatta atcatctggt gatgaagatt atgttattga
    11221 gttgtataaa aaataatcga atgaacatat gccaaaaata atcaccaatc agtattattg
    11281 cagattaaac aaataaaaat ctttccataa caaatggtta ttcattaatc ctgccataaa
    11341 ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt gcgcaacata
    11401 ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta caaaccaacg caacacaatt
    11461 catgccctgg cagtatgtca cgttctcgcg tttctgaacg gggaacggcg ctccattgag
    11521 gaagttattc tgatgaaaag tataaaattc agcctggcct ggcagattct gtttgctatg
    11581 gtgctgggca ttctcctggg aagctacctg cactaccata gcgacagccg cgactggctg
    11641 gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc atctgatcaa aatgattgtt
    11701 gtgccgattg tgatctccac gctggtggtg ggtatcgcgg gtgttggtga tgccaaacag
    11761 ctcgggcgta ttggcgcgaa aaccattatt tacttcgagg tgatcaccac cgtcgccatc
    11821 attttgggga tcactctggc gaacgtcttc cagcccggtg ccggggtgga tatgtcgcag
    11881 ctggcgaccg tcgatatctc gaaatatcag agcactacgg aagcggtaca aagcagttcc
    11941 cacggcatta tgggcacgat tttgtcgctg gtgccgacga acattgtggc gtcgatggcg
    12001 aaaggcgaaa tgctgccgat catcttcttc tcggtgctgt ttggtctggg gctttcctcc
    12061 ctgcccgcga cgcatcgtga accgctggtg accgtgttcc gctccatctc tgaaaccatg
    12121 tttaaagtga ctcacatggt gatgcgttat gcaccggtgg gtgtgtttgc gctgattgcg
    12181 gtgacggtgg ctaactttgg tttctcttct ctgtggccac tggcgaaact ggtgctgctg
    12241 gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg gaattgtggc gcgtctgtgc
    12301 gggttaagcg tctggatcct gattcgtatt ctgaaagatg agctgattct ggcgtactcc
    12361 actgccagct ctgaaagcgt actgccgcga attattgaga agatggaagc ctacggagca
    12421 ccggcgtcga tcaccagctt cgtggtgccg accggttact cttttaacct tgatggttcg
    12481 acgctgtatc aaagtattgc cgctattttc atcgctcagc tgtatggcat tgacctgtcc
    12541 atctggcagg aaatcattct ggtgctgacg ctgatggtga cctcgaaagg gattgctggc
    12601 gtgccgggcg tgtcgtttgt ggtgctgctg gcaacgctgg gtagcgtagg tattccgctg
    12661 gaaggcctgg cgtttattgc tggtgttgac cgtatcctcg acatggcgcg tactgcgctg
    12721 aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca agtgggaaca caaatttgac
    12781 cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca aatttgataa aactgcggat
    12841 caataattga agattgccgg ggttatccac ccggcaatgt gtggatgcct gatgcgacgc
    12901 tggtgcgtct tatcaggcct accttaaccg ttggttaatt tttcaaactc ttcacacccg
    12961 gtatcaaacc cttccataca gctcaggttc aaccagtgca gcgccttttg tttgttcttc
    13021 tcgataaaac cttcctcacc gtttaagaac atcattcccg cccagtactc ggaataaccg
    13081 gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc gtcggcctta
    13141 atgcccacgc cgttggcgta aatcaaacca agcagcattt gggcatcgac ggcggagtca
    13201 ttctccagat cttccgaggc gttttctaac agcgaaatcg cctttggata atccggttta
    13261 cccgcttgag tatttaccag aatatgcgcc agcgttactt caccttcttt actgcccgct
    13321 tgcgccgctt tctctgcaag aacttttgct tgtggatagt ccagactgac cggattggtg
    13381 attttgatct gcgccagcag cgcgcaggca tcagcatcac cgctgtcggc ggctttctgt
    13441 gcccaatatt cggctttgct caaatcgccg gagctaaacc agctgtcggc aagaaaatat
    13501 tgtgcgcgtc ggtccccggc ctctgctgcc tttaaatact ggctgccagg ctcggagtcg
    13561 ttcgcgtggg caaagaatgt caaaaacaac atcaatgcga taattttttt cattttgata
    13621 atacgattgg gtagggagta accagtataa agagttcgaa tggataaaaa aacagcctcc
    13681 gaaaggaggc tgtagaaagg actgtattac gccagtgccg cttcgcgcag gcgagttttc
    13741 aacttgttgt actcgtcaat cacgtactgc tcggcggcgc gctgatcggc gatcggctcg
    13801 acgcgcacgg cgcagtattt gtattccggc gttttcgtaa tcgggcttaa gttttcggta
    13861 accagctcgt tacaggcacc aatccaccac tggtaggtca tgtaaatcgc ccctttgttc
    13921 ggacgatcgc tgacctgcgc acgggtgata attttgcctt tacgcgagtg cacccaaacc
    13981 aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg tattgatttg tgcgtagcca
    14041 ggttcatcag ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg gcaagagtag
    14101 tgaccaactt cacgcaccgt tgacagtacc atcgggtact cgtcggtgag tttgtcgatt
    14161 ggcgctaccc agtcgcaggt gaagaactgt gccagaccgt tcggggtatc aaacttctct
    14221 ttaaacagat aagaagtccc ctgatcggca tctgaagtat cgcggcaagg ccactgaatg
    14281 aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca cagatgacgc
    14341 aactcatccc agatctcctg ggtgttgttg tagtgcatcg gataacccat acgggtggcg
    14401 atttcactga tgatttgcca gtccgttttc agatcccatt tcggttcaac tgctttgaag
    14461 aaacgctgga agccacggtc agccgcagta aacacgcctt catgctcgcc ccacgacgtt
    14521 gacggcaaaa tgacatccgc cgccgacgcg gttttggtca taaagatgtc ctgaacgata
    14581 accagttcca gatcttcaaa ggctttacgt actgccgaca gctccgcgtc agtttgcagc
    14641 ggatcttcgc ccataatgta cgcggcacgc acttcgccat gcgctgcgcg gtgcggcagc
    14701 tcgctgatgc gataaccggt atgagcaggc aggctttcca cgccccaggc tttggcgaat
    14761 ttctcgcggt tagccggatc tttcacgtac tggtatcccg gatacgtatc cggcagcgcg
    14821 cccatatcgc aggcaccctg cacgttgttc tgaccacgaa ccgggtttac acccgcatgc
    14881 ggcttaccga ggttaccggt cagcatcgcg aggctggtca gagaacgcac ggtttccacg
    14941 ccctggtaga actgggttac acccatgccc cacaggatgg cggcgctttt cgcctgggca
    15001 tacatccgtg ccgcctgacg aatctcactg gcgctgacgc cggtgatatc ttcaaccgac
    15061 tccggcgtgt agccttcaac gattttacga tactcttcaa agccttctgt acgggaagcg
    15121 acgaacgctt tgtcgtacag attttcttca ataatgacat ggcccatcgc attcaacagc
    15181 gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc ggtttcaatt
    15241 ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt tagcgttaat tacgtgattc
    15301 gccacgattg ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa atcggtatta
    15361 tcaatttcgt taatagcatt gctcattgcg ccattaccga ccgattggtg cagacctgca
    15421 accgatgggc cgtgtcagac acgagcgcag cagtcaacgt tattggtacc aataacggcg
    15481 cgcgcaaatt tttgcattac atagttggtt tcgttacccg taccacgcga ggagccggtc
    15541 gtctggatgg catccggacc gtacttctct ttgatggcgc tcaggcgctc ggcaacgtaa
    15601 ttcagtgcct catcccagga aacaggttcg agtttgccgc cacgctggcg acggatcatg
    15661 ggggttttca ggcgcggggt caggatctgg gtatcgttaa tgaaatccca gccataataa
    15721 cccttcagac acagggtacc ctggttggtt ttcccctgcg ctgcctccgc ccggacgatt
    15781 ttgccgttat cgacgaccag gttgattttg caacctgatg cgcaataggg gcaaaccgtg
    15841 acgacttttt tcatcggtct cgctccagtt aatcaaatca cgcatacgcg ctctcgacta
    15901 cagtatgcat cttttatgcc acattttatt tggggtcatt ccctgatatt acgggcacta
    15961 tttattcaaa actctgacga aaaacaggct gtcgtcagtt ttgacgtgac gaaacgaaat
    16021 accgcgtgac agccatcacg cggcagacat tttatttttt ctcgacgacg ggagccgcct
    16081 gatacccgcc gcccagcgat ttcatcaact gaatgctttg gatcacccgg cggctgtcca
    16141 gcatcagtaa tgacatctct tcggcaagta ctggcaaccg ggcttcagtg gcctgtaagc
    16201 ggctggttaa gccgcgctga taggcggcct cggcagcgcg ctgggtgaag cgcgttgctt
    16261 ccacgcgttc agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc gtgccgttga
    16321 cggcaacgtc acgcaccgcg ttcagtactg actggttgta acgttcaatc atcatgttgc
    16381 tggcggcgcg cgtgccttcg agattggcat tcaaccgtcc accgtcaaac agcggcaatt
    16441 tcagacccgg gatgaagttg aactggcgac tggttttttt gaataaggta tccaggtgga
    16501 tggcatcaag gccgaaaaac gctttgatat caaagctcgg gtagaacagc gcccgcgcgg
    16561 aatccacctg atctaatgac gcctgaacat accagcgcat ggcttgcaga tccgggcgtc
    16621 tggcgagcaa ctcataagag agtgtcgccg gaatgccggt ctggactcgt ggtaatgcca
    16681 ccggtttgat ctccggcata tcgctggccc ccgcgccaat caatgcacgc agagattctc
    16741 gcgtttcggt gatttgccct ttgacggcag caatttgttt atcgaccgcc agaatctgtg
    16801 cccgcgcgcc ctggaaaggc acttgtgctt ccagaccgtg cgccacttta ctctggtgcg
    16861 ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa cagatcgagc atctgatagc
    16921 tggcctgcat actgtaataa agctgcgcta cgcccgtggt cagcgatagc tctactgctg
    16981 cggtttctgc cagcgtggca ttatgcgcgc caatggcggc ggcaaccgct gagcgatgca
    17041 caccccacaa atcaagatcc agtccggcaa acaaagctac tgtggcttcc gtatagtacg
    17101 gcccgtccat acccagtgcg ggcgcatcca tcgcataagg gcttaaaaag ccgttcgccg
    17161 agacgcgttg gcggttgagc atccctaacg ctgcgacctg taattgtgaa ccggcatcta
    17221 acaaatcggc ctgggactgc gctttttctt cccgcagttt cgcttcggcg agggtgtgtg
    17281 aaccacttag cgtccgttgg agcagcgcat ccagctgcgg gtcattgagt tgtttccacc
    17341 actgcgcctg cggccagccg gagctggcaa gatgaatatc gtcggccagt ttgatttgtt
    17401 ccggtttgag ctgttgatga ggtgcagaat ccttacgtac cagggcacag ccggaaatca
    17461 gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga aagttgacga ttgatcattg
    17521 cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc agtctggcaa
    17581 gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat aaggtgggca ggatggcctg
    17641 cgaggcgggt gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg ttgccagacc
    17701 ggcagcgtat ttctccaggg cgtcggcaaa ctgagctgct ctgtccggct gtagtgcgtt
    17761 atccagcgcc tgcgccgagt tccaggtggc atcccaggcg tgaagaatat cgcggccctg
    17821 acgaataacc gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt ccagttgacg
    17881 ctccagcgcc acgcgttggc acatctcttc acaggcatta aacgccgcat gcagaccgat
    17941 acgaatttgc agataagtgc gcagagccgt gacttcctgc tggcgtggga tccgcattac
    18001 tttacttaac atacccagcg tgccagccag tttttgcggc agtgtgcgcg cttcactttc
    18061 aggccagaca aaggtgtaaa tcaccgcgga caccacggta ccaatgatga tacccagggc
    18121 gcgatcgcga atttccacca ggtcgtacac tgggccaaaa acgttttcga gcgtggcgag
    18181 cgcgaaggtg accaccatct gtgtgccgat ataagaagag cgttcagagc tggtggcaat
    18241 ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc aattcgacaa tattgtccag
    18301 ccagggcatg accagtagcg tgaataacag cgccagaatc gcgccgcaaa aggccccgcc
    18361 aaaacgcagc accatcttct ggtacgacga accgacattt gggttagcga cgatcacgca
    18421 tgtcagcata caggtgtgaa tgccttccca gtccacgccg ctgtaaaagg tgtaacagat
    18481 caaacaggcg agcagcgttt ttaccgcgta gcgcatatag tctggattgg taaaagcatc
    18541 ggcggccatt gatggcggtt tggctgcggg cgttggcggc gtattcgggt ccatctgacc
    18601 cagttgtaac aacgtctggc agatattctc caggttacat tcccgtgccg ccatcgcttc
    18661 actttcactg atccgccagt cgctttgcca gcactgacct tcagcaacgg catgctgcag
    18721 cttattgatt tctgaggcta atttttgccg aaattcaata attgcctgag aatcagcaaa
    18781 agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg ccacgcagct
    18841 ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc gcgagacaaa agacattcag
    18901 tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg cgaggctgtc
    18961 cgtcaggtgg ctaatggcat catcaagccg atcattaagc gcctgatgca tttgcgaaat
    19021 ggcacgactg ggaaaccaca gcacgccgat taacgtcatc agcaaggttg gataaaggcc
    19081 aacaacgata caccacagcg ttaagcgcac gaccacttcc ggatagtcga gcatggcggg
    19141 gaaggtttgc ccgtaaatgg cgacaatggc gactgcgaaa aagaccagtc ccagccgatg
    19201 ggtgcgcatc aaaaacatac agcccatcag gatcggcccg gcgatgaaca atcggatcaa
    19261 cggttcgccg tatgaccatt tgtagatcaa aaacaggctg ccgatctcca gcatcgtggc
    19321 gaccacaaat aagatcgcga caaatttggt gtaaaacgcg ttcgactgaa taccgtaaaa
    19381 cagcactgcc agcgataacg ccacgaaagg gatctcaaag gtcatcgaga tcagaatcac
    19441 cagcaggcag cctacccaga gttgcacggt ctgcggcacg cggcctggtc gccgctcgct
    19501 taactcttca tgaaagaacg ccagcagcct gaccaccggt aatggcaggg agttgagcgc
    19561 gctcattatt gcggctcaag attagcgact gccgaagcgc cgatgcggaa catttccggg
    19621 tcaggtttat cgaccatgat tttgaccgga aaacgctggg caacgcggac ccagttaata
    19681 gagcgagaaa ctttcggcag gccgcccagc accaggccgc cgtcatccgg tagcacgccg
    19741 tagccaatcg aatccacttt accctcgaag gttttgccgc tgtcactcat caggcgaatc
    19801 gttgcgggtg tacctgagcg aatatttttc agatcggttt cgcggaagtt ggcgatcacg
    19861 taccagtgac gagtgtcgat tagggtaaaa atagggcgca tggcagaagc aaattgcccg
    19921 acggaggttt tgagggaaat gacccggcca tcaaacggcg cgcgaacggt cgccatttcc
    19981 agatgcagtt tggtcagggc aatatccgct tcgaccgccg cacgctgggc aactaatgca
    20041 tccacgccgc tgacggcgct ggcggctgac tgcgcctgca acaatacagc attaagatcg
    20101 gcttctgcag cgcgctgcgc ggtcctcgca cggtcaacgt cttccgctga gacaaaacct
    20161 tctttcagta atggctcggt gcggcgtaat gtatctgtgg cctgtttcgc ggcggcacgg
    20221 gctttttcta ccgtggcgtt aaccgagtcg gcaccaaact gttgcgcgtc aacgctacgc
    20281 tgggtgagca taatttgctt atccagcgcc gcgagggagg cttcagcttt cgccagattg
    20341 gcttcgtacg ggcgcgggtc aatgcggaac agcaaatcgc cctgtttgac tgcctggttg
    20401 tcggtgaccg ccagttctac aatgcgaccg ctgacttccg gcaccacatc aatggtatct
    20461 gctgacgcgt aagcgtcatt agttgatggc gcactgtcta cgcgccagat aacgaaaaca
    20521 agggcaacca gcgccaacgc aaccactaac agagcaggga atttactgcg aggagctttt
    20581 ttcggcgtac tttccatgag cgcgtctcaa atagattaga aaaatgccag ccagaacagc
    20641 atggcgtaga gagcaaaaag ggcggtataa ataattccga caaatgccag attgatattt
    20701 gcccgctgaa taactcgcct cgtgatgagc atcaaaataa ggctggcaat ggcgcagaaa
    20761 aaccagctgg gataataagc accgatcacc gggattgccg gagataatga gcatcccgaa
    20821 accatgaaga cgggaattat ccaggctgtt tttttgagtt gcatcgccat gcgagagaga
    20881 actgttggca tattaaatac atcctgtctg ctgcgaacaa tatctgtagt gaatatcaat
    20941 atccattgaa ggcgtcagta tggctatttt ctcgatgaat aaaaatagct ttatt
//
LOCUS       DAZGOB010000080        20909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGOB010000080 DAZGOB010000000
VERSION     DAZGOB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.080"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            167..943
                     /gene="exuR"
                     /locus_tag="ACHLIF_003173"
     CDS             167..943
                     /gene="exuR"
                     /locus_tag="ACHLIF_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO9607143.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1183..1266
                     /gene="yqiM"
                     /locus_tag="ACHLIF_003174"
     CDS             1183..1266
                     /gene="yqiM"
                     /locus_tag="ACHLIF_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO9607144.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1288..1950
                     /gene="yqjA"
                     /locus_tag="ACHLIF_003175"
     CDS             1288..1950
                     /gene="yqjA"
                     /locus_tag="ACHLIF_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO9607145.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1954..2337
                     /gene="mzrA"
                     /locus_tag="ACHLIF_003176"
     CDS             1954..2337
                     /gene="mzrA"
                     /locus_tag="ACHLIF_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO9607146.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2484..2852
                     /gene="yqjC"
                     /locus_tag="ACHLIF_003177"
     CDS             2484..2852
                     /gene="yqjC"
                     /locus_tag="ACHLIF_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO9607147.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2890..3195
                     /gene="yqjD"
                     /locus_tag="ACHLIF_003178"
     CDS             2890..3195
                     /gene="yqjD"
                     /locus_tag="ACHLIF_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO9607148.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3198..3602
                     /gene="yqjE"
                     /locus_tag="ACHLIF_003179"
     CDS             3198..3602
                     /gene="yqjE"
                     /locus_tag="ACHLIF_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO9607149.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3592..3891
                     /gene="yqjK"
                     /locus_tag="ACHLIF_003180"
     CDS             3592..3891
                     /gene="yqjK"
                     /locus_tag="ACHLIF_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO9607150.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4077..4469
                     /gene="yqjF"
                     /locus_tag="ACHLIF_003181"
     CDS             4077..4469
                     /gene="yqjF"
                     /locus_tag="ACHLIF_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO9607151.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4539..5525
                     /gene="yqjG"
                     /locus_tag="ACHLIF_003182"
     CDS             4539..5525
                     /gene="yqjG"
                     /locus_tag="ACHLIF_003182"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO9607152.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5818..6183
                     /gene="yhaH"
                     /locus_tag="ACHLIF_003183"
     CDS             5818..6183
                     /gene="yhaH"
                     /locus_tag="ACHLIF_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9607153.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6425..6781
                     /locus_tag="ACHLIF_003184"
     CDS             6425..6781
                     /locus_tag="ACHLIF_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9607154.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(6832..7728)
                     /gene="yhaJ"
                     /locus_tag="ACHLIF_003185"
     CDS             complement(6832..7728)
                     /gene="yhaJ"
                     /locus_tag="ACHLIF_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO9607155.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            7833..8534
                     /gene="yhaK"
                     /locus_tag="ACHLIF_003186"
     CDS             7833..8534
                     /gene="yhaK"
                     /locus_tag="ACHLIF_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO9607156.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPDAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8557..8721
                     /gene="yhaL"
                     /locus_tag="ACHLIF_003187"
     CDS             8557..8721
                     /gene="yhaL"
                     /locus_tag="ACHLIF_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO9607157.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDETTA"
     gene            complement(8933..10243)
                     /gene="cyuA"
                     /locus_tag="ACHLIF_003188"
     CDS             complement(8933..10243)
                     /gene="cyuA"
                     /locus_tag="ACHLIF_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO9607158.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10271..11602)
                     /gene="cyuP"
                     /locus_tag="ACHLIF_003189"
     CDS             complement(10271..11602)
                     /gene="cyuP"
                     /locus_tag="ACHLIF_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9607159.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(11877..13241)
                     /gene="tdcG"
                     /locus_tag="ACHLIF_003190"
     CDS             complement(11877..13241)
                     /gene="tdcG"
                     /locus_tag="ACHLIF_003190"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO9607160.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13313..13702)
                     /gene="tdcF"
                     /locus_tag="ACHLIF_003191"
     CDS             complement(13313..13702)
                     /gene="tdcF"
                     /locus_tag="ACHLIF_003191"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO9607161.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13716..16010)
                     /gene="tdcE"
                     /locus_tag="ACHLIF_003192"
     CDS             complement(13716..16010)
                     /gene="tdcE"
                     /locus_tag="ACHLIF_003192"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO9607162.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVTTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16044..17252)
                     /gene="tdcD"
                     /locus_tag="ACHLIF_003193"
     CDS             complement(16044..17252)
                     /gene="tdcD"
                     /locus_tag="ACHLIF_003193"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO9607163.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGSSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17278..18609)
                     /gene="tdcC"
                     /locus_tag="ACHLIF_003194"
     CDS             complement(17278..18609)
                     /gene="tdcC"
                     /locus_tag="ACHLIF_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO9607164.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18631..19620)
                     /gene="tdcB"
                     /locus_tag="ACHLIF_003195"
     CDS             complement(18631..19620)
                     /gene="tdcB"
                     /locus_tag="ACHLIF_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO9607165.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19719..20657)
                     /gene="tdcA"
                     /locus_tag="ACHLIF_003196"
     CDS             complement(19719..20657)
                     /gene="tdcA"
                     /locus_tag="ACHLIF_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO9607166.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAVV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5417 a         5436 c         4951 g         5105 t
ORIGIN      
        1 tttagtagct ttcacaatga cgatacttat tttttaggaa tttataaata ttcacaatat
       61 taattcaata cattaattca attaccaaat ttatatatga ccaatggcat acccatgaat
      121 aaagtggtat gataacttcg tccgcattcc actccgggag acgcatatgg aaatcactga
      181 accacgccgt ttgtatcaac aacttgccgc tgacctgaaa gagcgcatcg aacagggcgt
      241 atatctggta ggtgataaac tgcctgcaga acgctttatt gccgatgaaa agaacgtcag
      301 ccgtacggta gttcgtgaag ccatcatcat gctggaagtt gaaggctatg tcgaagtgcg
      361 taaaggttca ggtattcatg tggtttccaa ccagccgcgc catcagcagg cggctgacaa
      421 taatatggaa ttcgccaatt acggtccgtt tgagttgctt caggctcgcc agctcatcga
      481 aagtaatatt gccgagttcg cggcaactca ggtaacgaaa caagacatca tgaaactgat
      541 ggccattcag gaacaggcgc gcggcgaaca atgcttccgt gattccgagt gggatttaca
      601 gttccacatt caggtagccc tggcgacgca gaactccgcc ctggcggcca tcgttgaaaa
      661 aatgtggacc caacgtagcc ataacccgta ctggaaaaaa ctgcacgaac acattgattc
      721 ccgtaccgtc gataactggt gtgatgacca cgatcaaatc ctcaaggcgc tgattcgcaa
      781 agatcctcat gctgctaagc tggcaatgtg gcagcacctg gaaaacacca agatcatgtt
      841 attcaacgaa accagcgacg acttcgagtt caatgccgac cgctatctgt tcgccgaaaa
      901 cccggtagtg catctcgata cggccaccag cggcagtaaa tgaaatacct gtccgacagg
      961 cgctgcgacg cgcctgtttg tctcgtcggt aagcgaaaag tataaagtgt cagcctgtgt
     1021 aaatcctctc gccaccctcc cctgcattca gcaaaatcag actccacgga catgtaattt
     1081 tgataacgaa caacgttgac ctttgttaca attagattca atttgaattt atgtttttga
     1141 atgctttctt atctcacgat ttaacaggga atagttcagg ctgtgttgat gtatcaaacc
     1201 cgcagaacat accaaaacag caataacatt gcggtagtgc atcttttaaa accagcgtgg
     1261 cgttaaccga ttcaccagga ataatgaatg gaacttttga cccaattgct gcaagccctg
     1321 tgggcgcagg attttgaaac cctggccaat ccatcgatga ttggcatgtt gtattttgtc
     1381 ttgtttgtaa ttttgttcct tgaaaacggc ttgcttccag cggccttttt accgggcgac
     1441 agtttactgg tattggtcgg cgtgttgatt gcgaaaggcg cgatgggtta cccgcaaacg
     1501 attctgctgc tgaccgttgc cgccagcctc ggctgctggg tcagctatat tcaggggcga
     1561 tggctgggca atacccgcac cgtacaaaac tggctatctc atttacccgc gcattatcat
     1621 caacgcgcac accatctttt tcataaacac ggtttatcgg cgctgttaat tggtcgcttt
     1681 attgcgtttg tcagaacact gctgccgacg attgccgggt tatcagggct gaataacgcg
     1741 cgctttcagt ttttcaactg gatgagcggt ctgctgtggg tattgatcct gacaactctg
     1801 ggttacatgc tcggcaaaac gccggtattt ttaaagtacg aggaccagct gatgtcatgc
     1861 ctgatgctgc tcccggtggt gctgctggtg tttggcctgg caggttctct ggtcgtgtta
     1921 tggaaaaaga aatatggaaa tcgggggtaa gggatgcaaa tacctcgcat gtcgcttcgc
     1981 cagctagcct ggtccggcac tgttttactt ctggtcggca cgctgttgct ggcctggtcc
     2041 gcggttcgcc agcaagagtc tacgctggcg attcgtgccg ttcatcaagg cacaacgatg
     2101 ccagacggtt tttcgatctg gcatcacctt gacgctcatg gcattccttt caaaagtatc
     2161 acccccaaaa acgacactct gttaattaca tttgactcca gcgaccagag cgccgccgca
     2221 aaagcggtcc tcgacagaac attgccccat ggctacatca ttgcgcagca ggacaataac
     2281 agtcaggcta tgcagtggct gacccggtta cgggataact ctcatcgctt cggataactt
     2341 cctgttatcc gaaacatatc actcactttg gtgatttcac cgtaactgtc tatgattaat
     2401 gaggcggtgg ttaacactac cgcttcgttg tctggacaat cgttcctttg taataggtcc
     2461 gaatcacaat ggaaggttca agaatgaaat accgcatcgc tttagctgtt tctctctttg
     2521 ctcttagtgc cggtagttat gccactaccc tgtgtcagga aaaggagcaa aatatcctta
     2581 aggagatcag ctatgccgaa aaacaccaaa accagaatcg tattgacggt ctgaataaag
     2641 ccctgagtga agtccgggcc aactgttcag atagccagct gcgtgccgat catcacaaga
     2701 aaatcgcaaa gcagaaagat gaggtggcgg aacgccagca agatttagcc gaggcgaagc
     2761 aaaaaggcga tgccgataag attgccaaac gcgaacggaa actggcagaa gcgcaggaag
     2821 agctgaaaaa gctggaagcg cgcgactact aactcacaat agtcactact tactcacctg
     2881 gagaaaacta tgtcgaaaga acacactacg gaacatctgc gtgctgagtt gaaatccctt
     2941 tccgatacgc tggaagaggt gcttagctca tctggcgaga agtcgaaaga agagttgagt
     3001 aagattcgta gcaaagcgga gcaggcactg aaacagagcc gttatcgcct gggtgaaacc
     3061 ggtgatgcca tcgccaaaca aacccgtgtc gcggcggcgc gtgctgatga gtatgtgcgt
     3121 gaaaatccgt ggaccggcgt gggcattggc gctgcaatcg gtgtagtgct cggcgttctg
     3181 ctgtcgcgtc gttaattatg gcggacactc atcacgcgca agggcccggt aaaagcgttc
     3241 tgggcatcgg gcagcgaatt gtttccatca tggttgaaat ggtggaaaca cgtctgcggc
     3301 tggcggtggt ggagctggaa gaggaaaaag ctaatctctt tcaactttta ctgatgctgg
     3361 gcctgacgat gcttttcgct gcatttggtc ttatgagcct gatggtgcta attatttggg
     3421 cggttgatcc gcaatatcgc ctgaatgcga tgattgccac caccgtggtg ttgctgctac
     3481 tggcactaat tggcggtatc tggacgttac gtaaatcgcg taaatccacc ttgctgcgcc
     3541 atacacgcca tgagctggca aacgatcggc aactgctcga ggaggagtcc cgtgagcagt
     3601 aaagtcgaac gtgaacgacg taaggcgcaa ctgcttagcc agatccagca acaacggctg
     3661 gatctttccg ccagtcgtcg tgaatggctg gaggcaacag gcgcttacga tcgtcgctgg
     3721 aatatgctgc taagtctgcg ctcctgggcg ctggttggca gtagcgtgat ggcgatctgg
     3781 acgattcgcc atcctaatat gctggtccgc tgggccagac gcggttttgg cgtatggagc
     3841 gcctggcgtc tggttaaaac gaccctcaag cagcaacagc ttcgcggtta acttccctct
     3901 ggccggagca attccggcct tatccctcaa atttcttgaa gatttttgac agttttcctt
     3961 gctaacaatc atcattcacc acgtttatga ttctctccat cgacagcaac gacgctaata
     4021 ccgcgccctt gcacaaaaaa acaatcagca gcctgagtgg cgcagtggag agtatgatga
     4081 aaaaattaga agatgttggt gtactggtag cgcgcatttt aatgccgatt ctgtttatta
     4141 ccgcaggctg gggaaaaatc accgcttact ctggtaccca acaatatatg gaagcaatgg
     4201 gtgtcccggg ttttatgctg ccgctggtga ttctgcttga gttcggtggt ggtctggcaa
     4261 tcctgttcgg tttcctgact cgcaccacag ccctgtttac tgcgggcttt acgctgctga
     4321 cggcattttt atttcacagc aactttgctg aaggcgtcaa ctcgctgatg ttcatgaaaa
     4381 acctgacaat ttctggcgga ttcctgctgc tggcaattac cggtccgggc gcgtatagca
     4441 tcgaccgcct gctgaataaa aagtggtaag cacgcgctat acttaacgat aaaaaacgag
     4501 gaggaagctc ctcgtttttg ctattggagg agagaaaaat ggggcaactg attgacggcg
     4561 tctggcatga cacctggtac gataccaaat ctaccggcgg taaatttcaa cgttcagctt
     4621 ccgcatttcg taactggctc actgccgatg gcgcacctgg ccccactggc acaggcggtt
     4681 ttatcgcaga gaaagatcgt tatcatctct atgtttcact cgcctgcccg tgggcgcacc
     4741 gcacgctgat catgcgcaaa ctcaaaggac tggaaccgtt tatttccgtt tccgtagtga
     4801 acccgctgat gctggaaaac ggctggacct ttgatgacag ttttccggga gcaaccggag
     4861 atacgctcta tcaacatgaa tttctgtatc agctttatct ccacgccgat ccacactaca
     4921 gcggacgagt tactgttccc gtgctgtggg acaaaaagaa ccacaccatc gtcagcaacg
     4981 aatcagcaga aatcatccgc atgttcaata ccgcgtttga tgcgctgggc gcaaaagcgg
     5041 gtgattacta cccaccagcc ctgcaaacga aaattgacga acttaacggc tggatttatg
     5101 acaccgttaa caacggcgtg tataaagctg gttttgccac cagccagcaa gcctacgacg
     5161 aggcggtggc gaaagtgttt gaatcgctgg cgcgactgga gcagatttta ggccagcatc
     5221 gttacctgac cggcaaccag ctaaccgaag ccgatattcg cctgtggacc acgctggtcc
     5281 gttttgatcc agtgtatgtg acccacttca agtgtgataa gcaccgcatc agcgattacc
     5341 ttaatctgta tggcttcctg cgtgatatct accagatgcc gggaattgcc gaaacagtca
     5401 atttcgatca catccgtaat cattacttcc gcagccataa gaccatcaac cctacgggga
     5461 ttatttcaat tggtccatgg caggatctcg atgaaccgca tggacgagat gttcgcttcg
     5521 gttaaaaaaa ggcaccttac gaggtgcctc tgataattag ctaagtgcat gttgttcatg
     5581 tcggatgcgg cgtgaacgcc ttatcaaacc tacaaaacat ttatattcaa tatgttgcaa
     5641 tgaccgcgca ggccagataa gcgtagcgca tctggcgatt ttgcgcttgt catcagtctt
     5701 agcgcgcctt cacagcactt tattaattcc ttgaacgaat atttactgcc atttcattca
     5761 cgtctattct taatttgctg ctttaaaaac aagtgattga gcaaaattga ggcaaaaatg
     5821 gactggtatc tgaaagtact aaaaaattat gtcggtttcc ggggtcgtgc gcggcgcaaa
     5881 gagtactgga tgtttattct ggtcaacatc atctttacgt tcgtgctggg gctgctggat
     5941 aaaatgttag gctggcaacg cgctggtggc gaaggcatcc tgacgacaat ctacggtatt
     6001 ctggtgtttt taccgtggtg ggcagttcag ttccgccgcc tgcacgacac cgaccgctcg
     6061 gcgtggtggg cactgttgtt cttaatcccg tttatcggct ggcttattat catcgtcttt
     6121 aactgccagg cgggtacgcc aggcgaaaac cgctttggac cagatccgaa actggaacct
     6181 taaaacaaat ctgctcgccg gatgtctgac tcatccggct tttatcatca tgttgatgaa
     6241 tgaataattt actaatgttt atttaaaata tttcaatgtc gttatataaa ttacctataa
     6301 aaaacaacca tgtatttatc ttaaatatca aaacctttat aacatccgaa aaagaaagat
     6361 acgaatagca tttacacaat acccggaata gactttcgtc acttttaatt aaagggatgt
     6421 ttttatgcag tggtatttag ccgtattaaa aaattatgtt ggtttctctg gtcgcgcacg
     6481 tcgtaaagag tactggatgt ttactctgat taacgccatc gtcggcgcta ttatcaatgt
     6541 cattcaattg attttaggtt tagagtttcc attcctttct ctcatttacc tggcagcaac
     6601 aatcattccg gtcattgctc tgtgtgttcg tcgtctgcac gatactgacc gttcaggtgc
     6661 gtgggcgctt ctctatctgg taccgatcat tggctggtta gttctgtttg tatttgcctg
     6721 tttagaaggc aattctggca gcaatcgtta cggaaacgat cctaagtttg gttcaaatta
     6781 atcttagaat tggggcgaca tttcgcccct ttttattaat aataacaaat attattttcc
     6841 gctaaaaagt ttgggaattt cccgcagaca ccaggatttc gcttccccca tactgtcacg
     6901 acgccaggcc ataataatat cgatctcgct ggtcgattcc gggctgacga cacgcaaccg
     6961 cccttccgca atatcttttt cgaccatcgg atacggcatc gtcgccacgc caagccccgc
     7021 cagtaatgcc tgacgtttat cttcaatcgt gctcaccgtt aagcgcggct gtttgtccag
     7081 cagctgtacg gtcaacaccg ggcgctcacg agcggtatcc gctaccgcaa ttccacgata
     7141 tttcacgcgc gtcacttcag ataacggttc cggctcctga tgaatcgggt gatcaggcgc
     7201 ggcaacgtag acgttcatta acgtatagag cttgcgcgag ttgatctccg acgaggaacg
     7261 aaaatgcata tccggcgcga taacaatatc cgcccgcccc tgctccagtc gttcccacgc
     7321 ccccgccagc acttcggtga tgattgccag ttgggtattg gcttttgccg ccagtttgtc
     7381 gattaacggg aaaaaggcag gtgtcggtac cagcgcttcg gtcacaatgg tgagatgcgt
     7441 ttcccaaccg cgcgcgaggg cttccgcatc ggtagtcagt ttatctgcgg cttccagcaa
     7501 aacgcgccct cgctccagca acatcctccc gacattggtg aatttggtgc gatggcccga
     7561 gcggtcaaac agcaccacat ccagctcttc ttccagtttt tgcatggtgt agctaagtgc
     7621 agaaggcacg cgtcccagct catccgccgc cgccgcaaaa ctgccccggc gatcgatcgc
     7681 atccataacc cgtagtgctt ccagcgttaa tgccctttct ttggccattt cgttctcatt
     7741 caggaaattt gaacataccg ggcagaatat ctggctaaca atgtagcgtc cagcccctta
     7801 ccataaaagg aagtaaagag aggtcaagaa ttatgattac tacccgaact gccaggcagt
     7861 gtggacaagc agactacgga tggttgcagg cccggtatac tttttccttt ggacactact
     7921 tcgacccgaa attgttaggc tatgcctccc tgcgtgtgct taaccaggaa gtgctggccc
     7981 cagatgccgc ctttcagccg cgaacctatc ccaaagtcga tattttaaat gtgattctgg
     8041 atggagaagc agagtatcgc gacagcgaag gcaatcatgt tcaggccagc gccggtgagg
     8101 cgttgctgct ctctacccag ccgggtgtca gctacagcga acacaatctc agcaaagaca
     8161 aaccgttaac acgaatgcag ctttggctgg acgcctgccc gcagcgagag aatccgctga
     8221 ttcaaaaact ggcgcttaat atgggcaagc agcaattaat cgcctcgcca gagggtacga
     8281 tgggaagcct gcaattacgc cagcaagtgt ggctgcacca tatcgtgctc gacaaaggcg
     8341 aaagtgcgaa tttccagttg catgggccac gcgcgtattt gcaatcgatt cacgggaaat
     8401 ttcatgcgct tacgcatcat gaagagaaag cggcgctgac ctgcggtgat ggggcgttta
     8461 ttcgtgacga ggctaacatt acgctggttg ccgattcccc actgcgcgct ttgctgatag
     8521 atttgcctgt ctagttgttt ttacagggag atgatgatga gtaaaaaatc ggccaaaaag
     8581 cgccagccag tgaagcccgt ggtggcgaaa gaacctgctc gcaccgccaa aaattttggc
     8641 tatgaagaga tgttgagcga gctggaagcc atcgtcgcgg atgctgaaac gcgtttagcc
     8701 gaggatgaaa ctaccgcgta aagacaggtt gccggatgcg gcgaaaacgc cttatccggc
     8761 ctacaaaatc caacaacatt aacgaattaa acaacccgta ggccggataa gatgcgccag
     8821 catcgcatcc ggcatcaggt acaaaacaca acaacatcaa caagttgagc accccgtagg
     8881 ccggataagg tgcgccagca tcgcatccgg cacgataccc aaaacctggc gtttatctgg
     8941 ccttgctcgc cataatctcg ataatctgcc gatccgtttg ctgcatcgaa tggcttgcta
     9001 acgcacacag gttggcaatc gactgctcaa catcatgcgc cacgatccct tcattgccgg
     9061 tcacggcggt atcatccagc gccattaaca ccgctttcca cgcagccgaa gcactggtcg
     9121 aaaccttcat cgcgcagctg ttcgacgcac catcgcaaat catgccgctg acatcgccga
     9181 tcatactgct gatcgccatc gagatggttt cataacgccc atccaccagc catgccatcc
     9241 cggcggcggc ccccattgct gcggtcgttg cggcacacag cgcagacaaa cgcggtaact
     9301 ggttatggat gtaaattgcg ctcaaatgag aaagcatcag cgcacgcgcc agccgttcat
     9361 catccgctcc gaagtgttct gctaccacca ccacaggcat tgttgcggtg atcccctggt
     9421 tacccgagcc ggagttactc atagccggaa gcgtagcgcc gcccatacgc gcatcggatg
     9481 ccgcgctggt acgaatcaca atggatgaag agagatcttt cgccagcaaa ccgcgctcgc
     9541 actgtttttc cagcgtcgcg ccaatatgca gcccccactt accgctcaaa ccttcctgcg
     9601 ataacgcaca atttagcttc gcggaatcga gaataaagcg gatcgccgca aacgggactt
     9661 cattgacgaa cttcaggatc tcagccagcg tcgttctgga aagcaccgtc agcggagact
     9721 cttgctcgcc ctctgccaca cacgcctgct gggtaaacac cacaccattg tgcgtctcaa
     9781 tatgcacaat gttggtatgc ccgccgacga tggtgacaca cgcccacttc tcaccgttcc
     9841 agactttggc gcgtgagaag aggatttcat tgcaaggttc ctggatctta acggagactt
     9901 tccccgccgc cagcagtgct ttggcatcgg caattgcctg cgcagttgcg tctttcagca
     9961 cttccagccc ggcgttggca tttccaccta acgcccccag cgccgccgca atcggcagcc
    10021 ccaccattcc cgtgccggga acggtgacgc ccagaccgtt cttcatcaga tttggcgaaa
    10081 cccaggcttc tacacgttca accggacctt ccagttctgc cgcagcaacc gccgccgcca
    10141 gcgccagtga aatcggttca gtacatccca gcgccggttt tacttcctcc tgaacggcga
    10201 ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa catattaaaa accttaaaat
    10261 ttcaggtaaa tcaagaaaat gccaggaacg gagaaacaca aagcaacaaa ccagtgacga
    10321 taatcaggta cagagacatc cctttgtatt tgtgcaatgc cggtactttg taaaccagcc
    10381 acgccgggat caggcaccct accatgccga aaatcgggct acagatagag gtgaagctca
    10441 acaccggtgc gttcagtacg atggcgctcc aggccagcaa aatggcgaaa atcatgatgc
    10501 cgcgctgaac aagattttcg ttaatcttct cggcaggcat cttgcgacgc aggatgttca
    10561 ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac gccaaagaat gcagtcatga
    10621 cagcaaagat attgagaatg acgctgacca ctttcaccca cgctgcgccg tcaccgctaa
    10681 taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc ataggctttt accgcttcgt
    10741 catgtcccat cgccagcgtg aacgacacgg cgtagaaaaa gacggtgaca aacaaaatgc
    10801 caaacgcgat attcattgcc cgcaatgctt tatgccgcgc cacttcaatt gatttttccc
    10861 gcgagcgata agagatcacc atcgggctta acgtctggat aaacagaatc gacgtcaggg
    10921 taaacggcag cgtaataatg gcgtttttca ccagcagccc cagcggcggt agcgaaccga
    10981 cgttatacag atgccacatt cccaccatcg acacgcccag cgccgcgacc accagcagct
    11041 tggtcagcac catgccggtc gaaattttga ataacaattt ctcgccgcgt gaagagatcg
    11101 ccaccagaat gcaaatcagt accagaccat aaaagggact gtctgacagc aacccttccg
    11161 tcacgccgaa ggtatgcaga taggaagcac tatcgttggt gatggcggtg gaatagacga
    11221 acatccaaat caccagcatt acgaaataga gcgcacctaa caggatgccc cagtttttac
    11281 ctaaataacc gctaatgaca ctcgggtaat ctttacattc tggtgattct gccagcgtat
    11341 taataaacaa ccgctgaaac agatacattg ccgggtaacc aatcaccgat gagagcaaaa
    11401 atacccacaa tcccatcaaa ccgacctgca ccgggagaaa aacaatcccc gcgccaatcg
    11461 ccatcccgat actcataatc acccagccgg tgtcagtact gtcgaatttg atcgcttctc
    11521 gccactcgct ctcactcatt ccagcacgac ccgccggggt cgaagcgtct gcaatgacgc
    11581 ctttattcga tgcaatttcc ataatttctc gctcaatatt ttgtagggct tattattttt
    11641 ttccgagccg catcaaggcg atatgcggtc tacgtttttg caggcgagtt attagaataa
    11701 agaaatgata cgcgcgaggt cagagaaaat cttcacaatc aagactttga aataacggag
    11761 atggataaga attttctact aaattaatcg caagagaata ttcattctct atttgcggca
    11821 gatcacaaaa aaaaggtgca catttgtgca cccaaggatg aaagctgaca gcaatgtcag
    11881 ccgcagacca ctttaatggc cagtcctccg cgtgatgttt cgcggtattt atcgttcata
    11941 tctttgccgg tttcatacat cgtctcgatc actttatcga gtgaaacacg cggtgccgag
    12001 gtgcggcgca tcgccatccg cgcggcgttt actgctttca cggcattaat ggcattacgt
    12061 tcaatgcacg ggatttgtac ctgtccggca accggatcgc aggtcagccc aaggttatgc
    12121 tccatcgcga tttccgccgc attgcatacc tgcgccggac tgccgcccag tagttcagtt
    12181 aaccctgccg ccgccattga acaggccacg ccaatctccc cctgacagcc gacctccgcg
    12241 ccagagatgg aggcgttcat tttatacagc gcgccaatag cccccgctgc cagaaaatag
    12301 cgggcaattg accgctcgtt taccggacga cggaacttat cgtaataagc cagcactgcc
    12361 ggaataatgc cgcacgcacc gttagtcggt gccgttacca cgcgcccgcc agctgcgttt
    12421 tcttcactaa ccgccagcgc gtacatgttg atccagtcga tgacattcat cggatcgtta
    12481 gagatgttat cgctggaaac cagctgacga cgcagcgcta cggcacgacg cggcacattg
    12541 agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt caataccgtc atgcatcact
    12601 tgccagatac gggcaaaccc tgcgtcaatt tccgctttgc tgcgcagcgc cagctcgttg
    12661 tgcatcatca ggccagatat agacaggccg ttgtaatcac acattttcag cagttcacct
    12721 gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt gcgacaggcc gaagtgttct
    12781 tcttcgacaa taaacccgcc gccgacagag taataggttt tacttaatag cgcttcctgt
    12841 cccttccagg cagtgatccg cattccgttc tcatggcgag gcaacatttc gggatggaag
    12901 ataatgttct ttgctacagg aaaatcaaca atatgcgcac cagatgccac tggcagccgc
    12961 ccgctgcgcg ttactaactc tataaatgca gggatctcat caatgacaac atcctgcgga
    13021 ctgtttcctg ccagtcccat gataatggcg acatccgtgg catggccttt gcccgtcagt
    13081 gacaacgacc catacagatc gaccacaata tggctcgtcg cggttaataa gccgctactt
    13141 tccagccgat caataaaact ttttccggca ttcattggcc ccacggtatg cgaactggag
    13201 ggaccaatcc caattttgaa aatatcgaat gcactaatca tatccacacc ctcggattgc
    13261 cgttcagtga agtggagcgg aacgaccttg cgaccgtccc gctcacgagg ctttacgcac
    13321 tacgtactgc gatagcttca atttccagct tcacatcttt cggcaaacgc gcaacctgca
    13381 cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa gaactgctta tagacttcat
    13441 tgatggtggc aaaatcattc agatcggtga tgaacacggt catcttgatg atatcgccca
    13501 cgctcagccc ggcagcaacc acgatcgctt tgacgttttc gaggcttaaa cgcgcctgat
    13561 cttgcacatc agccgggatc tcaccggtct gcgggcaaac cggaatttgc ccggaggtga
    13621 agaccatgct gcctaaatca acgccctgaa cataagggcc gattgcgcct ggggcacgtt
    13681 gcgtttcgat aatctttttc atacatcctc cggcgtcaga gcgcctgggt aaaagtacgt
    13741 gaaataacat cctgttgctg ttcacgggtc agtgcgttga agcgcacggc gtagccagag
    13801 acacggattg tcaggttagg atatttttcc gggtgctcga tggcatccag cagcatttcc
    13861 cgattcatta cgttgacgtt gaggtgttga ccgccttcga catccgcttc gtggtggaaa
    13921 tacccatcca gcaggccgac aaggttggtt ttacgtactg gatcttcttt gcccagcgcc
    13981 gcaggaacga ttgagaaggt gtacgagatc ccatctttgg cgtaggtgaa cggcagtttc
    14041 gccaccgacg tcaatgaggc cacggcacct ttgcggtcac gaccatgcat cgggttagcg
    14101 cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg tattaccggt tttctggccg
    14161 tacaccacgt tagaagtgat agtcagaatc gactgggtag tgacggcgtt gcgataggtt
    14221 ggcagcgctt taattttctt cataaagcgt tcaaccaggt cgcaggcaat gctgtctacg
    14281 cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga tttcaaagtc caccgccagg
    14341 ccgttttcgt cacggattgg tttcacgcgg gcatatttga tggcagacag ggagtccgtc
    14401 gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat agacatcacg atcgtgcagc
    14461 gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt agtgaatgat attcagcgcg
    14521 ctgatgtact gcaccgccag ccagtccatg aagtgatcga ggctgtccat cactttgtcg
    14581 taatccagca cgtcgtccat cagcggtgct gttttcggcc cgacctgaat cttcagcttc
    14641 tcgtccaccc cgccgttaat tgcgtagagc agcgttttcg ccaggttagc gcgtgcacca
    14701 aagaactgca tttgcttacc aatcaccatt gggctgacgc agcaggcaat cgcgtaatcg
    14761 tcgctgttga agtcagtacg catcagatcg tcattttcat actgcaagga agaggtgacg
    14821 atcgacacct gcgcggcata ttttttgaag gcaatcggta attcttccga ccaaagaatg
    14881 gtcaggttag gttccggtgc cggccccata gtgtgcaggg tgtgcaaata gcggaaggag
    14941 tttttggtca ccagcgtacg accgtccagc cccatcccgc cgatcacttc cgtcgcccag
    15001 attgggtcgc cggagaacag cgaatcaaat tccggtgtac gcaggaagcg taccatacgg
    15061 atcttcatga tgaagtgatc gatcagttcc tgtgcctgct gctcattgag tacgccagct
    15121 ttaaagtcgc gctcaatgta gatatcgagg aacgatgccg tgcggcccag cgacatcgcg
    15181 ccgccatttt gcgatttcac tgccgccaga taagcgaagt agagccactg caccgcttcc
    15241 tgcgcattct gcgccgggcg agagatatca aagccatatt tcgccgccat ttcctgaatc
    15301 tgcaacagcg catgacgatg ctctgccagc tcctcacgca gacggatggt ggcttccaga
    15361 tcctcgcctt tttccagacg agactggaga tcggcaaatt gcagttcgcg ttcacgtacc
    15421 agataactga tgccatacag cgctacgcgg cgatagtcac cgataatgcg cccacggcca
    15481 tagccatctg gtaaaccggt cagcacgcca gatttacggc agcgcagcat atccggtgag
    15541 taaacatcaa atacgccctg gttatgggtt ttacgcagat cggtaaacag atattcaaat
    15601 tcactgtcca tttcacggcc ataggcgtgg aatgaacttt taatcatatt aatgccaccg
    15661 aacgggtgta gcgcacgttt caacggcgca tccgtttgca ggccaacaat tttttccagc
    15721 ggctggttaa tatatcccgc atcatgagcg gtaattgtgg tggcaatatt ggtatcgaaa
    15781 tcaaccggcg cgtgggttgc gttttcgata cggatacctt ccattacttt ttcccacaat
    15841 tccgtggtgg caggcgtcgc ttcggcgagg aaagattcat cgccttcata cggtgtatag
    15901 ttatgttgaa taaaatcgcg gacattaatt tcgtttttcc agtccgtacc tttaaagcca
    15961 agccatgcgt cggcgtacag cttatcgctg gtatcaatat ctaccttcat gaaaaataat
    16021 ctctctacaa tacttcaact aaattatgca aattctgcgg gcgcgttaac tttgcctaaa
    16081 tgaatggcat ccaaagcaat cattttttct tcgttagtcg gaataacagc gcaaatgaca
    16141 cgcgcatttt cactggaaac aattcgctca ccacaggagt tagagcgatt attcatttct
    16201 gtatcaatct ctacgcctaa tacagccaaa tgttccatga ccagacgacg aattaagctt
    16261 gaattctctc ctattccgcc ggtgaatata attccatcca ggcgacgtaa tgaagctgcg
    16321 tgtccggcaa tatgacgggc aattcggtga acaaaggttt taattgccag ttgcgcgcgt
    16381 tcgtgacctt catgccaggc tttttccaga acacgtaaat ccgaagaaag accggaaata
    16441 cctaataatc ccgactcttt attcactacg cgttccaggt cacccaggct ctggttggtt
    16501 tggctggcga cccaggacat cgcaccaaag tcgacatcgc cactgcgggt acccatcatc
    16561 aagccttcca gcggcgtcat tcccattgaa gtatcaacac tctgaccgtt gcgaaccgcg
    16621 cagattgacg cgccattgcc aagatgcgcc acaaccaggc cggagtcatc ttccgccaga
    16681 ttcagcagcg aatgtgcgcg ctgggaaaca tagcggtgcg acgagccgtg gaaaccataa
    16741 cggcgtacac ctaactcttc ataatatttc cacggcaggc cgtataaata agcttccgga
    16801 gccatcgtct ggtggaaact ggtatcaaat accgccacct gagttacgcc cggaaataat
    16861 tgctgcgccg attcaatacc acttaaattg gcgtaattat gcaggggtgc cagtggagaa
    16921 acgcgacgga tattatcaat gacttcatcg gtaataatgg cggactcggt aaaaatactg
    16981 ccgccgtgag cgatgcggtg gccaattaag gccacactgt catttaaatt ccgtttttcc
    17041 agttcaaatg caattgcctt caatgcacct tcgtagctgt ggtgagccag cggtgctggc
    17101 tctcccccat ttacggataa gaatgcattt tccgagttaa taccgtcggc aatacctgac
    17161 attaatactt cacagtcgct ggcatcgagt acggaaaact taatcgaaga cgaaccacag
    17221 ttaataacca aaacaaccgg aaattcattc atctcttttc tcatcctgag ttacggatta
    17281 aaacagtttg tatacgatgt tcaggatggt cagcagacca atcacggtaa caaacacgtt
    17341 atccagacga ccacggtatt tcgccagaga cggcgcttta cggatggcat acatcggcaa
    17401 caggcacagc agggatgcga taatcggtgc gcccatggct tcaataaggt cgaggatgtt
    17461 cgggttggcg taggcaacaa cccaggtgga gcccatgatg aagatcatgc tgatagtgtt
    17521 cagtttaccc agcgacactt tagttttgtc gcctttataa ccgaacttca gaatcagacc
    17581 attcaagcct tccagcgtcc ccagatagtg accgaagaaa gatttgaaga tagccacgag
    17641 tgcgatgatg gaagccgcat attccagtgt aatcgcgaac gttgttttgg taccggtcat
    17701 ggacgcaaag tggttagcca gataagaaag cactggaata ttctgcgctt tggcttccgc
    17761 catgttggcc ggagacagcg taaacaggca gctaaaggca aagaacatca ccactgcaac
    17821 catcagcatg ctggcacgag aaatgatttg ggaacattta cgttcggtga agtcgcgacc
    17881 gaagtctttc tcatactctt cacgtttaga aaccacgaag gaagagacga ttggcgagaa
    17941 gttaaaggag aaaaccatga tggaaatccc cagccagaca gtgatcagga taccgtcatg
    18001 accggttaac gacagcgaac cgaggtcaac ctggtcgata actgcagagt tccagtaagg
    18061 gatcagcgac agagaaatca gcaccaggct ggcgataaac ggccatacca ggtagctcat
    18121 cactttaacc atcagatcct taccaaacca gatgacgaaa gccatcagca gcaacaggaa
    18181 cagcgccaca aagccgcgat tcagcggtgc aaatccgagc tggttttccc agaacgtcat
    18241 aaaggtattg gtaatagtaa cgccataaat ccacagcagt gggcaaatcg cgaagaagta
    18301 caggaacgtg ataaccacgc cgccagtttt accaaaatgc tcttccaccg tttccgtaat
    18361 gttgccggaa gggttagagc cagaaagaca cagacgcgcc agcgcccggt ggcaataaaa
    18421 tgcgatgggg tatgccaata ccaacatcag aagaatcggg atcagtccgc caaaacctgc
    18481 gcggataggg aagaacagca ccccggcgcc gattgccgta ccaaacaagc ctaacgtcca
    18541 tgtggtatct gatttacgcc aggacgattg ttttgtctgg ctggatacaa tgctatctga
    18601 agtactcata tcctatcctc aacgaattaa ttaagcgtca acgaaaccgg tgatttgaga
    18661 gacgcgagaa agatcgatat tgccgccgga aataatactg acggtttttc tgttttgaat
    18721 atattggtct aatttaccgc ttaataatgc agcacatgcc agagcgcctg cgccttcggt
    18781 gacgacttta ttgcgctgaa ttaaggcaat catactgttt ctgatttcgt cttcgctgac
    18841 cagcacgatg tcatcgacta attcacgaac gatttcgtaa gttaaattac ccgggcggga
    18901 gacatcacaa ccatccgcca gggtgccggt agttcggtgc gtggttattt ctccggagtg
    18961 gaaagaagcc gccatgccgt gaacgttttc agactgtacg ccaataacac gaatggtcgg
    19021 gttaatagat ttaattgcca ccgcaatacc agcaattaaa ccgccaccac caattggcac
    19081 aatcacgtta tcgacatcat agagatcttc cataatttcc agaccaatcg ttccctggcc
    19141 agcaatcact ttcggatcat cgtaaggtgg gataaaaata cggccttcca tttcgacaat
    19201 ttcgctcact ttagcgatag tgtcgttgaa attatcacca tgcagaacga cttctgcgga
    19261 gtagtcgcac gttgccgcta ctttggattt tggcgcacct tttggcatca ccactttacc
    19321 gtcgataccc agcatcgcgc aggagagcga aaccccttgc gcatggttgc ccgcagaaca
    19381 ggccactaca cctttacgtt tttccgcatc agtcagtgaa cttaatttat taaatgcgcc
    19441 acgaatttta aatgaacccg tacgctgcat attttcaaac ttcaggaata tttcaccttt
    19501 gcaacgttca ctaaaatagt ttgagcgagg catgcctgtt ttataaattc gcccagccag
    19561 tcgttgtttc gcttcaataa tgtcatcaat agcaaccggc agatcgtatg taatatgcat
    19621 tataaaacct cttcgcctga attaaatatg taggtaaccg taaccgacac ctgcaaaaca
    19681 gacaggtgga ttatttatgt ttaaataaaa acaaataact aaccaacttc tattaattgc
    19741 cttcgtctac acccattata agatgaatac tctttggcta attccaccaa aaccgatgct
    19801 gcttttttaa tacgataatt tttcgaccat accacggcat attgtgccac aggtaatgtt
    19861 tcttcaaccg gaatagtaat aaattgattt gaaccaaaag gtgacgtcat atcacaagga
    19921 attacagtta agaaatcagc attgagaaca agattataaa ttgtcacgac tgagtcggtt
    19981 ttaacgatgt tttcaatact gatgccattt ctttgtaacg tagtaagaag ctcgctgtag
    20041 taccccatat tggtttgtgg caacacccac tgttcgttct tcaacgactc cagcgtggtg
    20101 gtgccggtgc atgttcggga cttactggct accagcacaa actcggactc gaacagcggc
    20161 tcaacatgta aatcctgaag cttcatttct gcacttaacg taccaatcgc aaaatccagt
    20221 cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg cttcatacat agaaacctgc
    20281 gctttcggga acacctcttt gaacttgttg atcatccctg acataaaagt aaaaccaatc
    20341 aatgaaggaa aaccaaatga gacttccacc accgcctcag aagacatacc gcttatctca
    20401 ttaaccatat ttttcatttc acgggtaatg gattcggaac gggagagtaa caattgaccg
    20461 gcaggtgtta atgttacacc ggtatttttc cgcaccacta attccacacc aaaataatct
    20521 tcaatatcgt taatgatttt actgacggcc ggttgagtta accctaattc ttttgcagcc
    20581 gagccgatag agccacttct aatgacttcc tgaaagacta ccaggtgctg cgttttcgga
    20641 agaagaatag tgctcataac gacctacgtt aattacctca ttgacggcat gaagtgtatc
    20701 aaaatgaaat gaacaggata tgtgcgacca ctcacaaatt aactttcaat actttccatg
    20761 gtatcgttac gcaaattaaa tacctttaaa tatcaacaag ttaaagtata aaaatcagca
    20821 taaaccctga tttttgtcaa aaaaaatgac ggggataacc atattttatg gcgataacat
    20881 cattcgttat gtggttaata taaaaaagg
//
LOCUS       DAZGOB010000081        20761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGOB010000081 DAZGOB010000000
VERSION     DAZGOB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.081"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            32..249
                     /locus_tag="ACHLIF_003197"
                     /pseudo
     CDS             32..249
                     /locus_tag="ACHLIF_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            359..622
                     /locus_tag="ACHLIF_003198"
                     /pseudo
     CDS             359..622
                     /locus_tag="ACHLIF_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165851.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            846..971
                     /locus_tag="ACHLIF_003199"
                     /pseudo
     CDS             846..971
                     /locus_tag="ACHLIF_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(1031..1537)
                     /gene="yciE"
                     /locus_tag="ACHLIF_003200"
     CDS             complement(1031..1537)
                     /gene="yciE"
                     /locus_tag="ACHLIF_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO9607167.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1583..2083)
                     /gene="yciF"
                     /locus_tag="ACHLIF_003201"
     CDS             complement(1583..2083)
                     /gene="yciF"
                     /locus_tag="ACHLIF_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO9607168.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(2169..2348)
                     /gene="yciG"
                     /locus_tag="ACHLIF_003202"
     CDS             complement(2169..2348)
                     /gene="yciG"
                     /locus_tag="ACHLIF_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO9607169.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(2729..3535)
                     /gene="trpA"
                     /locus_tag="ACHLIF_003203"
     CDS             complement(2729..3535)
                     /gene="trpA"
                     /locus_tag="ACHLIF_003203"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO9607170.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(3535..4728)
                     /gene="trpB"
                     /locus_tag="ACHLIF_003204"
     CDS             complement(3535..4728)
                     /gene="trpB"
                     /locus_tag="ACHLIF_003204"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO9607171.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(4740..6101)
                     /gene="trpCF"
                     /locus_tag="ACHLIF_003205"
     CDS             complement(4740..6101)
                     /gene="trpCF"
                     /locus_tag="ACHLIF_003205"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO9607172.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKSSPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(6102..7697)
                     /gene="trpD"
                     /locus_tag="ACHLIF_003206"
     CDS             complement(6102..7697)
                     /gene="trpD"
                     /locus_tag="ACHLIF_003206"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO9607173.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(7697..9259)
                     /gene="trpE"
                     /locus_tag="ACHLIF_003207"
     CDS             complement(7697..9259)
                     /gene="trpE"
                     /locus_tag="ACHLIF_003207"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO9607174.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGMENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(9281..9374)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(9351..9395)
                     /gene="trpL"
                     /locus_tag="ACHLIF_003208"
     CDS             complement(9351..9395)
                     /gene="trpL"
                     /locus_tag="ACHLIF_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO9607175.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            9533..10414
                     /gene="rnm"
                     /locus_tag="ACHLIF_003209"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             9533..10414
                     /gene="rnm"
                     /locus_tag="ACHLIF_003209"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO9607176.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10411..11031
                     /gene="yciO"
                     /locus_tag="ACHLIF_003210"
     CDS             10411..11031
                     /gene="yciO"
                     /locus_tag="ACHLIF_003210"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO9607177.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDKPVVVREGVGDVKPFL"
     gene            11132..12004
                     /gene="rluB"
                     /locus_tag="ACHLIF_003211"
     CDS             11132..12004
                     /gene="rluB"
                     /locus_tag="ACHLIF_003211"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO9607178.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(12044..12634)
                     /gene="cobO"
                     /locus_tag="ACHLIF_003212"
     CDS             complement(12044..12634)
                     /gene="cobO"
                     /locus_tag="ACHLIF_003212"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO9607179.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(12631..13389)
                     /gene="yciK"
                     /locus_tag="ACHLIF_003213"
     CDS             complement(12631..13389)
                     /gene="yciK"
                     /locus_tag="ACHLIF_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO9607180.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            13609..14658
                     /gene="sohB"
                     /locus_tag="ACHLIF_003214"
     CDS             13609..14658
                     /gene="sohB"
                     /locus_tag="ACHLIF_003214"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO9607181.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(14694..14945)
                     /gene="yciN"
                     /locus_tag="ACHLIF_003215"
     CDS             complement(14694..14945)
                     /gene="yciN"
                     /locus_tag="ACHLIF_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO9607182.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            15325..17922
                     /gene="topA"
                     /locus_tag="ACHLIF_003216"
     CDS             15325..17922
                     /gene="topA"
                     /locus_tag="ACHLIF_003216"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO9607183.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            18132..19106
                     /gene="cysB"
                     /locus_tag="ACHLIF_003217"
     CDS             18132..19106
                     /gene="cysB"
                     /locus_tag="ACHLIF_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO9607184.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            19425..19565
                     /gene="ymiA"
                     /locus_tag="ACHLIF_003218"
     CDS             19425..19565
                     /gene="ymiA"
                     /locus_tag="ACHLIF_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO9607185.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            19568..19735
                     /gene="yciX"
                     /locus_tag="ACHLIF_003219"
     CDS             19568..19735
                     /gene="yciX"
                     /locus_tag="ACHLIF_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO9607186.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            19849..19944
                     /gene="ymiC"
                     /locus_tag="ACHLIF_003220"
     CDS             19849..19944
                     /gene="ymiC"
                     /locus_tag="ACHLIF_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO9607187.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            20108..>20761
                     /locus_tag="ACHLIF_003221"
     CDS             20108..>20761
                     /locus_tag="ACHLIF_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFO9607188.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
BASE COUNT         5154 a         5104 c         5328 g         5175 t
ORIGIN      
        1 attcatgaat gaaaaatagc tatatgcgta cattctcaac ctgcccgtca cgtagcaggt
       61 tgaatcactt tttcttgata aagggctggt cctgccactg ttgttgttgg gtgcgttaaa
      121 aatacactac taatatgccc ggcatgcggg caacagagtc ctgttcacga tcgccgccac
      181 cggaaatggc gctgcctcga tacctgccag ttcatggcac tattcgaagc cgatgttccc
      241 cacgttatat ggagtaatca ttggggttat gccgcagcac gttgtatgca agaatgtgct
      301 gcggctggtt tgtgagcttt cgatagtggt tgttattttt gcccttattt gttccggagg
      361 ccctggttca atggtccgtc tgccccctgt ggtgatgtca gcaaaatcag ccactgcgcg
      421 aaccacaata gcccgggaag atgctgaaga tcaccaggta aagctgtcag cgcagaaact
      481 ggaagaactg ctcgcatcaa tggttaagga tgaggttgat cgcaatgatg ggatttattg
      541 acgtcagcga gagcagaagg aagaactgaa taacctgaat gatttacgct caatcagagc
      601 gatgatgatt ggcagtaatt agcggtcaat ttcgggtttt ttggctgtgc gctccccgcc
      661 cagcattgcg cgtcaattag ccccaaaaaa aacgaaaaag ttacaatcaa gtaaaaaatt
      721 ccttctgcaa aactaaaaaa gatttcaata aaatcaggca gttaaaaaca taaaaaaaag
      781 gtacaaaaaa acatctcaaa gtcttgtttt ttgcttcaaa tacaattggt tattgtgttt
      841 tttgaaacct accagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
      901 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
      961 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     1021 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     1081 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     1141 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     1201 aggtgtaaca ggcgatttca aattgctcga agacatatcc gctaatagag cctttaacta
     1261 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     1321 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     1381 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     1441 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     1501 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     1561 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     1621 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     1681 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     1741 caatctcata atgctcgact ttctgcgctg cggcaatcag tgcggcatca cgcacttcgt
     1801 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     1861 atttcatgcg cttaattttc aggttcgatt cggactccac aacttgatca atacgttcaa
     1921 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     1981 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     2041 aaagcaggtg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     2101 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     2161 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     2221 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     2281 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaacc accacgatgt
     2341 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     2401 gacactcatt aaaattagtc gctaatgaga attagtcaaa ttaagcgcaa cgagaagata
     2461 gagggaaaat atattttgag gaacattctg gatatattaa caattacctg aggaataagt
     2521 gacttagaga aaattattga ttttactggt gttatgttgc gggattaatt tgtttgatta
     2581 tattactgtt gggcggaaaa atgacgtaag ttgacgctcg accggggtaa gcgaaacggt
     2641 aaaaagataa atattaaatg aatttaggat ttttccggct tcattaaaga aagttaaaat
     2701 gccgccagcg gaactggcgg ctgtgggatt aactgcgcgt cgccgctttc atcggttgta
     2761 caaaaacttt cagtgccgcc agcattttct ctggctcatt aatatgttgc tcgatgattt
     2821 taacaatggc cgaaccagaa atcgcgcccg cagctcctgc atcaatcgct gcttttacct
     2881 gatccggggc ggaaatacca aatccctgca atggaggtgc agcgttgtac tctttcagct
     2941 tcgcaaccag atgattgagg ggtaacgcgg cgcggttttc tgcgccggtc acgcctgctc
     3001 gtgacagcaa ataggtgtaa ccacgaccgt aagaggctat ctggcgcagc aggtcgtcat
     3061 cggcatttgg cgggcagatg aagataggtg cgacattatg acgcaacgcg gcctggcgga
     3121 agggcgcgga ctcttcaact ggcacatcgg caaccagcac cgaatcgacg ccgacttttt
     3181 cgcactgggc ataaaactca tcaatgcctt tgttaaacac cagattggca tacatcaaca
     3241 ggccaatggg aatggtcggg tgtttctggc gaatcagtgc cagcatttca aaacattgtg
     3301 ccggagtcac acctgccgca aaggcgcgca gagtggcgtt ttgaatcgtc gggccatccg
     3361 ccagtgggtc ggagaagggg atacctaact ccagcgcgtc agcaccggct tcaattagcg
     3421 tatcgataat tttcaatgac tgctcaatgc ccggatcacc gagcgtgacg aaaggaacga
     3481 atgcgccttc tttgcgctcc ttcaactggg caaacagaga ttcgtagcgt tccatcagat
     3541 ttcccctcgt gctttcaaaa tatcgtgaac ggtgaagatg tctttatcgc cgcgaccgga
     3601 aaggttaacc accagtagct gctctttatc cgggttttcg cgcatcattt tcaacgcatg
     3661 ggccagggcg tgggaggatt ccagcgccgg gatgatccct tcgtgcaggc acagcgtttt
     3721 gaaggcttca agggcttcat catcggtaat agacacgtaa tcagcgcgtc cagtgctgtt
     3781 aagatacgcg tgttgtgggc cgacagacgg gaaatccagt ccggcggaga tggagtaaga
     3841 ttcttcaatc tgcccgtctt cggtttgcat catcggcgct ttcataccga aatagatacc
     3901 cacgcgacca tgttttagcg gtgcgccgtg ctcgccagtt tcgataccgt gaccacctgg
     3961 ctccacacca atcaggccga cgttggtttc attgatgaaa tcagcaaaca tgccgatggc
     4021 attcgaaccg ccgccaacac aggcgataac ggcatccggc aggcgacctt ctctttccag
     4081 aatctgcgct ttggtttctt cgccaatcat ccgctgaaac tcacgcacaa tggtcggata
     4141 aggatgcggg ccagctgcgg tgcccagcat atagtgcgcg gtttcgtaac taccggacca
     4201 gtcgcgcagc gcctcgttac aggcatcttt cagcgtcgcg gaaccgctat gcaccgggat
     4261 cacttccgca cccattaagc gcatacgaaa aacgttaggc gactggcgtt caacgtcttt
     4321 ggcacccata taaatacggc atttcaggcc gagcagggcg ctggcaaggg ccgacgccac
     4381 gccatgctga ccggcaccgg tttcggcgat gatttcggtt ttacccatcc gcttcgccag
     4441 caacgcctgc cccagcacct ggttagtttt atgcgcgccg ccgtgcagca aatcttcacg
     4501 cttgagatac agcgtagtgt tcgtcccggc tgtaatgttc tggcatttgg tcagcgcggt
     4561 tggacgcccg gcatagtttt tcagcaggtc gttgaactga gcctgaaatt caggatcttt
     4621 ttgcgcactg acaaaagctt cttccagctg gcgcagagca ggcatcagga tttgtggcac
     4681 gtacatgccg ccaaactcac caaaataggg gttaagtaat gttgtcattg ttcctttcct
     4741 taatatgcgc gcagcgtctg gaaaaccgag gccaaaagac gtgcgtcttt gatgcccggt
     4801 tgcgactcta cagcagaatt aaaatcaagt ccggcgcagc cggtttgtgc cgcttccacg
     4861 cagttatctg cgcctaagcc ccccgccagc agaacgttgc caagcgattg accatttaat
     4921 agtgaccagt caaaacgttg cccgcttcca ccctggccgt tgtctaaaac atatttatca
     4981 acgtgctgaa actcgcgggc gggcagggtt tcaccgacgc ttaatgcttt ccagatggca
     5041 acatgtgctg gcagagcttc acgcagcgta tcgatataca gctgttcttc attaccatgc
     5101 agttgcactg ccgccagcga taacacctta gctttgtcca ccacatcggc aatatcgtga
     5161 ttgcggaaca cgccaacata ctgcaacggt gccgcagcca tcacttcctg cgcctgttca
     5221 acgttgacgc aacgcggtga tgtcgcaaca aaaatcaacc caccgtaaat cgcgcccgcg
     5281 tcataagctg ctttagcatc ttgcccacgc gtcaggccac atactttatt ctcacccagc
     5341 aacacccggc gcacggcggc gtgcaaatcg tcatgggcca tcaacgccga accaatcaga
     5401 aaaccgttag cgaagtggct taactcgcgc acctgagcgt aagtattgat gccggattcg
     5461 ctgattaccg tcacgttgtg ccccagtttc ggcgcaagct cgcgggtacg gttgagatca
     5521 atcgacaaat cacgcagatc gcggttgttg atgccaacga cctttgcccc caatgcaatg
     5581 gcacgctcca gttcctcttc attactgact tcggtcagca cacccatctc cagactgtgg
     5641 gcgacggcgg caagctggcg atattgttcg tcatccagta ctgaaagcat taataagcag
     5701 gcatcagcct ggtaatagcg cgccagatag atctggtaag gatcgataat gaagtcttta
     5761 cataaaatcg gctgcggggc gatttggctg acgatgggga ggaaatcaaa gctcccctga
     5821 aaatatttct catcagtcag caccgaaatt gccgaagcgt aatgtttata aatggcggca
     5881 atgcgtgctg gatcgaaatc atcacggatc acgccttttg acggcgacga tttcttgcac
     5941 tccagaataa acgccgtgcg cgcaccctgt agcgcatcat aaaaatgtcg cgtgctcggc
     6001 tgaacctcat tctgaaaact ggccagcggt tgctgctgtt tgcgggtttc tacccaaatc
     6061 gccttgtctg cgacgatttt cgctaaaacg gtttgcatca tttaccctcg tgccgccagt
     6121 gcggtaactc tgtcgtaagc ggaaccactg cgcagtacct caagaacggt ttgcgcattg
     6181 gcttgcagat cttcatggcc atgcaggcgc attaacatgg cgacgttcgc agcgacggct
     6241 gcttcatggg cggcgtcgcc tttaccttgt aacaagcgtg ttaaaatgtc acggttttct
     6301 tccggtgttc cgcctgccag ttgctcctgg tggtagggag tcaggccaaa atcttcagcg
     6361 gtcaattgat agctcttaat ttcgccgtca tgcagctcgg caacgattgt cggcgcgtgt
     6421 aatgaaactt catccatccc gccgctgtgc accaccgccg cgcgttgata ccccagcacg
     6481 cgcaaggttt cggcaatcgg cagcaccagt tccggactat aaacaccaat taacgccagc
     6541 ggcggatgcg ccgggttaat caatggcccc agcacattga acagggtgcg ggttttcagt
     6601 tgctggcgaa ccggcatcgc gtggcggaat ccggtgtgat acttcggcgc aaagaggaaa
     6661 catacaccta actcatccag cgcctggcgc gatttatcgg cgttcatatc aagattaata
     6721 ccgaacgccg ccagcagatc ggacgaacca gatttactgg agacgctacg gttgccgtgt
     6781 ttcgccactt tcagcccaca ggccgcggcg acaaacgcac tggcggtaga aatattgata
     6841 ctgttgctgc cgtcaccgcc agtaccgacg atatcagcaa acagataatc cgggcgcggg
     6901 aacggcgcgg cgttttccag tagcgcggtt gctgctccgg cgatctcgtt cgggtgctca
     6961 ccgcgaattt tcatgctcac cagcgccgcc gccagttgtt ccggcttcag ctcgccacgc
     7021 accaccgctg aaaacagctg gtggctttct tgttggctaa gcgtctgcgc ctgatacagt
     7081 ttttccagaa tcggttgcag cgtgttggct ggctctagtt tctgctgcgc ccaggccagc
     7141 gtttgttcca gcaggcgagc gccctgggtg gtgagaatgg attccggatg gaactggaat
     7201 ccacaaacgc gatccgcatc gtgacgtact gccatcacca tgccattaaa atgggcgttg
     7261 atggttaaac cggccggaat gttactgcca accagcgagt gataacgcgc caccggcagc
     7321 gggtttgtta atccggcaaa catcgcctga ccgtcatgtt caatgctcga cgctttaccg
     7381 tgaagaattt cgcccgcctg accgacatag cccccgtaag cttcgacaat tgcctgatgt
     7441 ccgaggcaaa tgccaataat gggcagcttg ccacgcaagc gggtgaggag ttccggcata
     7501 caaccggctt cgctcggcac accggggcca ggagaaagca tcagtaccgg attgctcatc
     7561 gtcgccaggc gttcaattaa ggtctgcgcc ggaatatggt tgcggtaaat caccacatta
     7621 tgcccattgc tgcgcaactg atctgccagg ttgtacgtaa aagagtcgat attatcgagc
     7681 agcagaatgt cagccatcag aaagtctcct gtgcatgatg cgcggtggca atagcgcgca
     7741 gtacagcgcg ggctttatta cgggtttcgt cggcttccga ctgcggaaca gaatcaagga
     7801 ctacgccagc accggcttgc acggtggcga taccgttttc caccagcgcc gagcggatca
     7861 caatgcaggt gtcgagatcg ccatgcgcgg tgaaataacc taccgcgccg ccgtagctgc
     7921 cgcggcgacg accttccgcc tcggcaatta actgcatagc gcgtactttc ggcgcaccac
     7981 ttaacgttcc catattcata caggcgcggt aagcgtgcag ggcgtcgaga tcgtggcgca
     8041 gctcaccgac cacgcgggag accaagtgca tcacgtaaga gtaacggtca actttggtga
     8101 ggtcggcgac gtagcggctg ccgggggtgc aaatgcgtgc cagatcatta cgggcgagat
     8161 ccaccagcat cagatgttca gaaagctctt tatgatcggt acgcatttcc agttcgatgc
     8221 ggctgtcgag gtctctgtcc agtgaaccat cggcacgacg accgcgcggt cgtgttccgg
     8281 caatcgggta gatctcaatc tggcggctgg tggcgtcata cttgagcgaa ctttccggcg
     8341 acgcgccaaa cagggtgaaa tcattatcct gcataaaaaa catgtacggg ctgggattac
     8401 tctttttcag cacgtaatag gccgccagcg gtgacgggca gggcagagag aaacggcgag
     8461 atggcaccac ctggaaaatt tctccggcgc gaatcgcttt ttgcaacaaa cgcactacgc
     8521 caccgaactc ttcatcgctc tggttacatt cacaacgcat atgcggcacg gaaaccaccg
     8581 gcagcggtgg cgcggtttcg gtcagttgct ggcgaagatc gttcaggcga gcagtgagac
     8641 gttgtttttc ttcttcattc ggagcaaaca ggctggcctg aatgcgggtg ctttttttct
     8701 gatggtcaat caccatcagc gtttcagcga gataaaaaca gaaatcagga cagttatttt
     8761 ccgctgacaa ttgcggtaaa tcttcaaacc ccgccacaag gtcataagag aacaggccgc
     8821 cgaagaacat tgcttctcgt tcttccttcg gtacattcag catattctgc aataagcgga
     8881 aagcgtcaaa aaccgaaagg gagcataagc gagcgtcttc atccagcagt ggactgacag
     8941 gggggaagcg cagcacacgg cagtttggta attgttcatt ttccataccc gcaggcaggg
     9001 cgttatccag tagtgtcagc agggcttcgc cgttgccgga aagtgcctgg attgtgacag
     9061 tgtcacctaa agctgtaatg cgcagcgcac tgtctaccag cagcaggctt tttaaatcat
     9121 ctttgctgtc gatatctgcg gattccagca gcagcgttgc cggacgatcc ccacacaact
     9181 ggtgaaaaag cgcggtggga ttgtcgcgat aagcgccttc gcaggttagc agttcgagag
     9241 tcggtttttg tgtttgcatt gttattctct aattttgttc aaaaaaaagc ccgctcatta
     9301 ggcgggctgg gtatctgatt gctttacgca tggtgaatac actgcccgtt tcaggaagtg
     9361 cgccaccaac ctttcagtac gaaaattgct ttcattgtcg ataccctttt tacgtgaact
     9421 tgcgtactag ttaactagtt cgatgattaa ttgtcaatag ctcatttcag aatatttgcc
     9481 agaaccgtta tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga
     9541 cacgaattat gcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct
     9601 gacgccagaa gcattggtgc accgtgcagt cgagatgcgc gtcggcaccc tggcgatcac
     9661 cgaccatgac accacagctg caatcgcgcc agcaagagag gaaatttcac gttcgggact
     9721 ggcgttgaat cttattcccg gcgtggaaat ttccacggtc tgggaaaatc atgaaattca
     9781 tattgtcggg ctgaatattg atattactca tccgctaatg tgtgagttcc tcgcgcagca
     9841 gacagaacgg cgcaatcagc gggcgcagct gattgccgaa agacttgaaa aagcgcaaat
     9901 acctggggcg ctggaaggcg cacaacgact ggcgcagggc ggcgcagtga cgcgcggtca
     9961 ttttgcgcgt ttccttgttg agtgcggcaa agccagttca atggcggatg tctttaaaaa
    10021 gtatctggcg cgcgggaaaa ccggatacgt tccgccacag tggtgtacaa tagaacaagc
    10081 tattgatgtc attcatcatt ctggcggtaa ggcggtgcta gctcatcctg ggcggtacaa
    10141 tcttagtgct aaatggctga aaagattggt tgcgcatttt gccgaacacc acggtgacgc
    10201 gatggaagtc gcgcagtgcc agcaatcgcc caatgaacgt acccagctgg cggcccttgc
    10261 gcgtcagcat catttatggg catcacaagg atctgatttt catcagccat gcccgtggat
    10321 cgaactgggt cgtaaactct ggctgcccgc aggcgttgaa ggcgtctggc agctatggga
    10381 acagccgcag aacaccacag agagggaatt atgagccagt ttttttatat tcatcctgat
    10441 aacccacagc aacgtctgat caaccaggcg gtggagatcg tgcgtaaagg cggggtgatt
    10501 gtttatccaa ctgattccgg ctatgcgctc ggctgtaaaa ttgaagataa aaacgcgatg
    10561 gagcgtattt gtcgtattcg ccagctgccg gacggtcaca actttaccct gatgtgtcgc
    10621 gatctttctg aactctcgac ctattcattt gttgataacg ttgcgtttcg tttaatgaaa
    10681 aacaacacgc cgggcaacta taccttcatc ttgaagggga cgaaggaagt gccacgccgt
    10741 ctgttgcagg aaaaacgcaa aaccatcggt atgcgtgtgc cttctaaccc gattgcgcag
    10801 gcgttgcttg aagcactggg cgaaccgatg ctttccactt cgctaatgct gccaggcagc
    10861 gaatttaccg aatccgatcc cgaagaaatt aaagaccgtc tggaaaaaca ggtagatttg
    10921 attattcacg gcggctatct cgggcagaaa ccgacaacgg ttattgatct taccgatgat
    10981 aagccggtcg tggtgcgtga aggcgtaggt gatgtgaagc ctttcttata agtgcgtaac
    11041 tctgtatact acgcggcctt aaaatggcgc tatacggcgc cattgattct cctgttcgac
    11101 gcctgtgaag gcgacactaa aaggaagctc tatgagcgaa aaattacaga aagtgctggc
    11161 ccgcgctggc cacggctccc gccgtgaaat cgaatctatt attgaagccg gtcgtgtgag
    11221 tgttgatggc aaaattgcta aactcggcga tcgcgttgaa gttacccctg ggctgaaaat
    11281 tcgtatcgat ggtcatctga tttcggtacg tgagtccgct gaacaaattt gtcgcgttct
    11341 ggcctattac aaaccggaag gtgagttgtg tacccgtaac gatccggaag ggcgtccgac
    11401 ggtgtttgac cgtctgccaa agctgcgtgg cgcacgctgg attgccgtag gtcgtctgga
    11461 cgttaacacc tgtggtctgc tgctgttcac caccgatggt gaactggcaa accgtttaat
    11521 gcatccaagc cgtgaagttg aacgtgaata cgccgtgcgt gtatttggtc aggttgacga
    11581 cgcgaaactg cgtgatttga gtcgtggcgt gcaactggaa gatggtccgg cagcttttaa
    11641 aaccatcaag ttcagcggcg gcgaagggat caaccagtgg tacaacgtga ctctgaccga
    11701 aggccgtaac cgtgaagttc gtcgtctgtg ggaagcggtt ggtgtgcagg tgagccgcct
    11761 gatccgtgtt cgttacggtg atatcccact gccaaaaggt ctgccacgcg gtggctggac
    11821 ggagctggac ctcgcccaga ctaactatct gcgcgaactg gtggagctac cgccagaaac
    11881 cagctctaaa gtcgctgtag aaaaagaccg tcgtcgcatg aaggcgaatc agattcgtcg
    11941 tgcggtgaaa cgtcacagtc aggtgagcgg cggtcgtcgt tctggcggac gtaataacgg
    12001 ttaatcaaag tatgccggac gtcatatccg gcatttttac agattagtaa tcaatcccta
    12061 tctgcgcttt tacaccggca tcaaacgcat gtttgacggg gcgtaattca cttaccgtat
    12121 ctgccagttc aagaatatcc cgatgacaac cacgacccgt gataatcacc gtctgttgat
    12181 gtggacgttc atttaacgcc tgcaccactt cttccagtgg caaatagtca tacgccacca
    12241 tatacgtcag ttcatcaagc aaaaccatat ccagtgagga atcagcaagc atccgctttg
    12301 catgttgcca gacttcgcgg caggcggcgg tatcagactc gcggttttgt gtatcccagg
    12361 taaagcccgt tgccatcacc tgaaactcaa cgccatgtgg ctccagcaga ttgcgttcgc
    12421 cattaggcca ggtgccttta ataaactgca cgacacctac tttttttccg tgaccaactg
    12481 cgcgtgttgc cgtaccaaat gccgcggtgg ttttgccttt tccattgccg gtaaagacga
    12541 tgataatacc gcgttcatcc tgggcctggg ccacacgagc atctactttt tctttcactc
    12601 gctgctgacg ctgttggtag cgttcatcac tcattgggaa attcctggtt tacggcccgg
    12661 ttgggcgtca aaggtcatgc cggttttacg gcggctgtca tcgcccatca gccagaggta
    12721 gagcggcatg atatcagcgg gtgttttaag tttctgtgga tcttcggtcg ggaaggcgct
    12781 ggcacgcatt gcggtgcgcg taccgcctgg gttaatgcag ttgacgcgca ggcgctgctg
    12841 atattcatcg gccagtacct gcatcatccc ttcggtggca aatttcgacg ctgcatatgc
    12901 accccagtta gctcgtccct gacgtccaac gcttgatgaa gtaaagacca gtgaaccggc
    12961 gtccgatttg agtaataaag gaagcagcgc ctgggtgagc aaaaaggtgg cattaacgtt
    13021 gacctgcatg acgtcctgcc agacctgcgg attttgttcg ctcattgggc aaacatcgcc
    13081 gagcaatccg gcattatgca aaacaccatc cagacgcgga taattaacgg taatgcgctg
    13141 tgccagttgt tggcaatctt cggacgtgca ggtcagcaaa tcgagaataa accactgtgg
    13201 ctgacgtcca gtttcttcgt ttatgtggct ggctacctga cgtaattttt cttcattacg
    13261 gcccaacaga atcactgtcg caccatagcg tgcatacgtc atcgcggctt cacggccaat
    13321 cccatcgctg gctcccgtca ccaggataat gcgatcattg agtaaatctt gttttggctg
    13381 gtaatgcatg gctactcctc aacgacgttg tctgtcgcgc tgccagtgta ctttatgact
    13441 ttggggcttt atgcctgaaa cagaagggca tttcaatcag cctggggcga taacaccgca
    13501 aaatggatac tttgtcatac tttcgctgca ataacatctc tgcgagacgg cttaacatgc
    13561 ctgttgtaaa ctgtgagcca aagcgttgtt taaccaaggt ggggactcgt ggaattgttg
    13621 tctgaatatg gtttgttttt ggcgaaaatc gttaccgttg tgctagcgat tgcggcgatt
    13681 gccgccatta ttgtcaatgt tgctcaacgt aataaacgcc agcgtggcga gttacgggtc
    13741 aacaatctca gcgaacagta taaggagatg aaagaagaac tggccgcggc gctgatggac
    13801 tcacatcagc aaaaacagtg gcacaaagcg cagaagaaaa agcacaagca agaagcgaaa
    13861 gcagcaaaag cgaaagccaa actgggcgag gtggcaactg acagtaaacc tcgcgtctgg
    13921 gtgctggatt ttaaaggcag catggacgcc catgaagtga actcgctacg tgaagagata
    13981 acggcggtac tcgcagcatt caaaccgcag gatcaggttg tgctccgtct ggaaagccct
    14041 ggtggcatgg tgcatggtta cgggttggcg gcttcgcagc tgcagcgtct gcgtgataaa
    14101 aacattcctt taactgttac ggtagacaaa gtcgctgcca gcggcggtta catgatggcc
    14161 tgtgtggcgg acaaaattgt ttccgcaccg tttgctattg tgggttccat tggggtggtg
    14221 gcgcaaatgc ccaactttaa ccgcttcctg aaaagcaaag atattgatat cgaactgcac
    14281 actgccgggc agtataagcg tactctgacc ttgctgggtg aaaataccga agaagggcgg
    14341 gagaaattcc gcgaagagtt gaacgaaacg catcagttgt ttaaagattt tgtgaagcgt
    14401 atgcgtccgt ctctggatat tgaacaggtg gcaacgggtg aacactggta cggacaacag
    14461 gcggtagaga aaggcctggt tgatgaaatc aacaccagtg atgaagttat tcttagcctg
    14521 atggaaggcc gtgaagtggt taatgtacgc tatatgcagc gtaaacgact cattgaccga
    14581 ttcaccggca gcgcggcaga gagcgccgat cgattgttgc tacgctggtg gcagcggggt
    14641 caaaagccat tgatgtaaaa gacaaacgcg aggctaagac ctcgcgtttt gctttaatca
    14701 accagatgat atttttctga aagcacatgg gccaggtgtt tgaacatatt aaacaccgcg
    14761 gtgcttttgg ctgttggcaa tccttgttca tctaaaaagt agtcgccggt aaataccagc
    14821 acgccattac gctgcgtgac gccggtggct tcaatccctg cgagcgtatc ctcatgctca
    14881 cgaatgattt tgttggcctc tttcaacagc gtttcgcggt cgatgggttg tgtctctttg
    14941 ttcattattt actccttaaa caaggacatt agtctacgcc aggcatggct tgcagacaaa
    15001 tataccacgc tggtggcaag agcgccttac tggtaacttt ggattttgca tgctaataaa
    15061 gttgcgtatc ggattttatc aggtacagtg tgacgctttc gtcaatctgg caatagattt
    15121 gcttgacatt cgaccaaaat tccgtcgtgc tatagcgcct gtaggccaag acctgttaac
    15181 tcagtcacct gaattttcgt gaacagagtc acgacaaggg gttgatatcc gcagagaacg
    15241 agtccatatc ggtaactcgt tgccagtgga aggtttatca acgtgcgacg cattcctgga
    15301 agaatcaaat taggtaaggt gaatatgggt aaagctcttg tcatcgttga gtccccggca
    15361 aaagccaaaa cgatcaacaa gtatctgggt agtgactacg tggtgaaatc cagcgtcggt
    15421 cacatccgcg atttgccgac cagtggctca gctgccaaaa agagtgccga ctctacctcc
    15481 accaagacgg ctaaaaagcc taaaaaggat gaacgtggcg ctctcgtcaa ccgtatgggg
    15541 gttgacccgt ggcacaattg ggaggcgcac tatgaagtgt tgcctggtaa agagaaggtc
    15601 gtctctgaac tgaaacaact ggctgaaaaa gccgaccaca tctatctcgc aaccgacctt
    15661 gaccgcgaag gggaagccat tgcatggcac ctgcgggaag tgattggggg tgatgatgcg
    15721 cgctatagcc gagtggtgtt taacgaaatt actaaaaacg cgatccgcca ggcatttaat
    15781 aaaccgggtg agctgaatat tgatcgtgtt aatgcccagc aggcgcgtcg ctttatggac
    15841 cgcgtggtgg ggtatatggt ttcgccgctg ctatggaaaa agatcgctcg tggtctgtct
    15901 gccggtcgtg tgcagtcggt ggcggttcgc ctggtggtcg agcgtgagcg tgaaattaaa
    15961 gcgttcgtgc cggaagagtt ctgggaagtc gatgccagca cgaccacgcc atctggtgaa
    16021 gcgttggcgt tacaggtgac tcatcagaac gacaaaccgt tccgtccggt caacaaagaa
    16081 caaactcagg ctgcggtaag tctgctggaa aaagcgcgct acagcgtgct ggaacgtgaa
    16141 gacaaaccga caaccagtaa acctggcgct ccttttatta cctctacgct gcaacaagct
    16201 gccagcaccc gtcttggatt tggcgtgaaa aaaaccatga tgatggcgca gcgtttgtat
    16261 gaagcaggct atatcactta catgcgtacc gactccacta acctgagtca ggacgcggta
    16321 aatatggttc gcggttatat cagcgataat tttggtaaga aatatctgcc ggaaagtccg
    16381 aatcagtacg ccagcaaaga aaactcacag gaagcgcacg aagcgattcg tccttctgac
    16441 gtcaatgtga tggcggaatc gctgaaggat atggaagcag atgcgcagaa actgtaccag
    16501 ttaatctggc gtcagttcgt agcctgccag atgaccccag cgaaatatga ctccacgacg
    16561 ctgaccgttg gtgcgggcga tttccgcctg aaagcacgcg gtcgtatttt gcgttttgat
    16621 ggctggacaa aagtgatgcc tgcgttgcgt aaaggcgatg aagatcgcat cttaccagca
    16681 gttaataaag gcgatgctct gacgctcgtt gaacttacac cagcccagca ctttaccaag
    16741 ccgccagccc gtttcagtga agcatcgctg gttaaagagc tggaaaaacg tggtatcggt
    16801 cgtccgtcta cttatgcgtc gatcatttcg accattcagg atcgtggcta cgtgcgagta
    16861 gaaaatcgtc gtttctatgc ggaaaaaatg ggcgaaatcg tcaccgatcg ccttgaagaa
    16921 aatttccgcg agttaatgaa ctacgacttt accgcgcaga tggaaaacag cctcgaccag
    16981 gtggcaaatc acgaagcaga gtggaaagct gtactggatc acttcttctc ggatttcacc
    17041 cagcagttag ataaagctga aaaagatccg gaagagggtg gtatgcgccc gaaccagatg
    17101 gttctgacca gcattgactg cccgacttgt ggtcgcaaaa tggggattcg cacagcgagc
    17161 accggggtat tccttggctg ttctggctat gcgctgccgc cgaaagagcg ttgcaaaacc
    17221 accattaacc tggtgccgga aaacgaagtg ctgaacgtgc tggaaggcga agatgctgaa
    17281 accaacgcgc tgcgcgcaaa acgtcgttgc ccgaaatgcg gcacggcgat ggacagctat
    17341 ctcatcgatc cgaaacgtaa gttgcatgtc tgtggtaata acccaacctg cgacggttac
    17401 gagatcgaag agggcgaatt ccgcattaaa ggttatgacg gcccgatcgt tgagtgtgaa
    17461 aaatgtggct ctgaaatgca cctgaaaatg gggcgtttcg gtaaatacat ggcctgcacc
    17521 aacgaagagt gtaaaaacac acgtaagatt ttacgtaacg gcgaagtggc accaccgaaa
    17581 gaagatccgg tgccattacc tgagctgccg tgcgaaaaat cagatgctta tttcgtgctg
    17641 cgtgacggtg ctgccggtgt gttcctggct gccaacactt tcccgaaatc gcgtgaaacg
    17701 cgtgcgccac tggtggaaga gctttatcgc ttccgcgacc gtctgccgga aaaactgcgt
    17761 tatctggccg atgcgccaca gcaggatccg gaaggtaata agaccatggt tcgctttagc
    17821 cgtaaaacca aacagcaata tgtctcttcg gaaaaagacg gaaaggcgac tggctggtca
    17881 gcattttatg ttgatggcaa atgggttgaa ggaaaaaaat aacctttaat tctgtcaggt
    17941 ttttataaac aaagggtcgc gaaagcggcc cttttttatt gcatattatt ttttcttcac
    18001 acctatacac taaggctata aatgatatag tggttatagt tagcaccttt tttattatta
    18061 aatcgtatta gtcacccgct aggtgtgacg aaaaaacgat gttctgatgg cgtctaagtg
    18121 gatggtttaa catgaaatta caacaacttc gctatattgt tgaggtggtc aatcataacc
    18181 tgaatgtctc atcaacagcg gaaggacttt acacatcaca acccgggatc agtaaacaag
    18241 tcagaatgct ggaagacgag ctaggcattc aaattttttc ccgaagcggc aagcacctga
    18301 cgcaggtaac gccagcagga caagaaataa ttcgtatcgc tcgcgaagtc ctgtcgaaag
    18361 tcgatgccat aaaatcggtt gccggtgagc acacctggcc ggataaaggc tcgctgtata
    18421 tcgccaccac gcatacccag gcacgctacg cgttaccaaa cgttatcaaa ggctttattg
    18481 agcgttatcc ccgcgtttct ttgcatatgc accagggctc gccgacacaa attgctgatg
    18541 ccgtctctaa aggcaatgct gatttcgcta tcgccacaga agcgctgcat ctgtatgaag
    18601 atttagtgat gttaccgtgc taccactgga atcgggctat tgtagtcact ccggatcacc
    18661 cgctggcagg caaaaaagcc attaccattg aagaactggc gcaatatccg ttggtgacat
    18721 ataccttcgg ctttaccgga cgttcagaac tggatactgc ctttaaccgc gcagggttaa
    18781 cgccgcgtat cgtcttcacg gcaacggatg ctgacgtcat taaaacttac gtccggttag
    18841 ggctgggggt aggggtcatt gccagcatgg cggtggatcc ggtcgccgat cccgaccttg
    18901 tgcgtgttga tgctcacgat atcttcagcc acagtacaac caaaattggt tttcgccgta
    18961 gtactttctt gcgcagttat atgtatgatt tcattcagcg ttttgcaccg catttaacgc
    19021 gtgatgtcgt tgatgcggct gtcgcattgc gctctaatga agaaattgag gtcatgttta
    19081 aagatataaa actgccggaa aaataatttc ttgcgttatt ttcggcacct tttatgtagc
    19141 gaaggtgccg gaatatattc tcttttgtta cttatttcta taccgtgcac aatattttta
    19201 tccctcatat ttatagggta aattacctgt aaagtagtgt ctaattaccc gcctcgcaaa
    19261 ctccagtttt caaatatcta tttcaggtca aaagattgaa ttattcactg gagacgattc
    19321 gtaaattcgc tggatttttg actaaagttt ctttaggatt tgtctcatcg tatgattaat
    19381 tacaccaatc gttcaacgtc ttgctgataa gtgatggcga ttatatgagg ttagcaatgc
    19441 cttctggaaa tcaggaaccc cgccgcgatc ctgaattaaa acgtaaagcc tggctggcgg
    19501 tttttcttgg ttctgcactt ttctgggtgg ttgtcgcact gctgatttgg aaagtgtggg
    19561 gataactatg gtcggtcagg agcaactgga gtcgtcacca ttatgccagc atagtgacaa
    19621 tgaaacagaa acgaaacggg aatgttccgt cgttattcca gacgactggc aactaacatc
    19681 gcagcagcaa gcctttatag aactgtttgc tgaagatgat cagccgaaac aataattatc
    19741 atcattctta ttacccattt ttaatgaatt aaagggcttt taatacaccg cagcaataac
    19801 agcttgagtt atctcaacac aaaataataa ccgttaaggg tgtagcctat gatcaacaca
    19861 aatatgaaat attggtcctg gatgggcgcg ttttctctgt cgatgctctt ctgggccgaa
    19921 ctcctctgga tcattactca ctgatccttg accccgctgc ggcggggttg tcatttgctt
    19981 tgccacaagg tttctcctct tttatcaatt tgggttgtta tcaaatcgtt acgcgatgtt
    20041 tgtgttatct ttaatattca ccctgaagag aatcagggct tcgcaaccct gtcattaagg
    20101 aggagctatg tcgtcaaccc tacgagaagc cagtaaggac acgttgcagg ccaaagataa
    20161 aacttaccac tactacagcc tgccgcttgc tgctaaatca ctgggcgata tcacccgtct
    20221 acccaagtca ctcaaagttt tgctcgaaaa cctgctgcgc tggcaggatg gtaactcggt
    20281 taccgaagag gatatccacg cgctggcagg atggctgaaa aatgcccatg ctgaccgtga
    20341 aattgcctac cgcccggcaa gggtgctgat gcaggacttt accggcgtac ctgccgttgt
    20401 tgatctggcg gcaatgcgcg aagcggttaa acgcctcggc ggcgatactg caaaggttaa
    20461 cccgctctca ccggtcgacc tggtcattga ccactcggtg accgtcgatc gttttggtga
    20521 tgatgaggca tttgaagaaa acgtacgcct ggaaatggag cgcaaccacg aacgttatgt
    20581 gttcctgaaa tggggaaagc aagcgttcag tcggtttagc gtcgtgccgc caggcacagg
    20641 catttgccat caggttaacc tcgaatatct cggcaaagca gtgtggagtg aattgcagga
    20701 cggtgaatgg attgcttatc cggatacact cgttggtact gactcgcaca ccactatgat
    20761 c
//
LOCUS       DAZGOB010000082        20719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGOB010000082 DAZGOB010000000
VERSION     DAZGOB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.082"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(402..1598)
                     /gene="hemY"
                     /locus_tag="ACHLIF_003222"
     CDS             complement(402..1598)
                     /gene="hemY"
                     /locus_tag="ACHLIF_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO9607189.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1601..2830)
                     /gene="hemX"
                     /locus_tag="ACHLIF_003223"
     CDS             complement(1601..2830)
                     /gene="hemX"
                     /locus_tag="ACHLIF_003223"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO9607190.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            complement(2852..3592)
                     /gene="hemD"
                     /locus_tag="ACHLIF_003224"
     CDS             complement(2852..3592)
                     /gene="hemD"
                     /locus_tag="ACHLIF_003224"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO9607191.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3589..4530)
                     /gene="hemC"
                     /locus_tag="ACHLIF_003225"
     CDS             complement(3589..4530)
                     /gene="hemC"
                     /locus_tag="ACHLIF_003225"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO9607192.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4917..7463
                     /gene="cyaA"
                     /locus_tag="ACHLIF_003226"
     CDS             4917..7463
                     /gene="cyaA"
                     /locus_tag="ACHLIF_003226"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO9607193.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDNDTPLLQQYFS"
     gene            complement(7503..7823)
                     /gene="cyaY"
                     /locus_tag="ACHLIF_003227"
     CDS             complement(7503..7823)
                     /gene="cyaY"
                     /locus_tag="ACHLIF_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO9607194.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7871..9196)
                     /locus_tag="ACHLIF_003228"
     CDS             complement(7871..9196)
                     /locus_tag="ACHLIF_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO9607195.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            complement(9193..10701)
                     /locus_tag="ACHLIF_003229"
     CDS             complement(9193..10701)
                     /locus_tag="ACHLIF_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO9607196.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(11039..11593)
                     /locus_tag="ACHLIF_003230"
     CDS             complement(11039..11593)
                     /locus_tag="ACHLIF_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO9607197.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11943..12146
                     /gene="yifL"
                     /locus_tag="ACHLIF_003231"
     CDS             11943..12146
                     /gene="yifL"
                     /locus_tag="ACHLIF_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9607198.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12183..13007
                     /gene="dapF"
                     /locus_tag="ACHLIF_003232"
     CDS             12183..13007
                     /gene="dapF"
                     /locus_tag="ACHLIF_003232"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO9607199.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            13004..13711
                     /gene="yigA"
                     /locus_tag="ACHLIF_003233"
     CDS             13004..13711
                     /gene="yigA"
                     /locus_tag="ACHLIF_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO9607200.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13708..14604
                     /gene="xerC"
                     /locus_tag="ACHLIF_003234"
     CDS             13708..14604
                     /gene="xerC"
                     /locus_tag="ACHLIF_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO9607201.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14604..15320
                     /gene="yigB"
                     /locus_tag="ACHLIF_003235"
     CDS             14604..15320
                     /gene="yigB"
                     /locus_tag="ACHLIF_003235"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO9607202.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREVEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15404..17566
                     /gene="uvrD"
                     /locus_tag="ACHLIF_003236"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15404..17566
                     /gene="uvrD"
                     /locus_tag="ACHLIF_003236"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO9607203.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17713..18477)
                     /gene="yigE"
                     /locus_tag="ACHLIF_003237"
     CDS             complement(17713..18477)
                     /gene="yigE"
                     /locus_tag="ACHLIF_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO9607204.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18639..18695
                     /gene="ysgD"
                     /locus_tag="ACHLIF_003238"
     CDS             18639..18695
                     /gene="ysgD"
                     /locus_tag="ACHLIF_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO9607205.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18847..19797
                     /gene="corA"
                     /locus_tag="ACHLIF_003239"
     CDS             18847..19797
                     /gene="corA"
                     /locus_tag="ACHLIF_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO9607206.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19839..20331)
                     /locus_tag="ACHLIF_003240"
                     /pseudo
     CDS             complement(19839..20331)
                     /locus_tag="ACHLIF_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
     gene            complement(20328..>20719)
                     /locus_tag="ACHLIF_003241"
     CDS             complement(20328..>20719)
                     /locus_tag="ACHLIF_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO9607207.1"
                     /translation="CIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGL
                     GISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWF
                     FLQRHKDFAFKPFFTNFNTIERIENYL"
BASE COUNT         4886 a         5291 c         5347 g         5195 t
ORIGIN      
        1 cattcataat aagtatgtgt aggatcaagc tcaggttaaa tattcactca ggaagttatt
       61 actcaggaag caaagaggat tacagaatta tctcataaca agtgttaagg gatgttattt
      121 cccgattctc tgtggcataa taaacgggta gatgctcatt ccatctctta tgttcgcctt
      181 agtgcctcat aaactccgga atgacgcaga gccgtttacg gtgcttatcg tccactgaca
      241 gatgtcgctt atgcctcatc agacaccatg gacacaacgt tgagtgaagc acccacttgt
      301 tgtcatacag acctgtttta acgcctgctc cgtaataaga gcaggcgttt ttttatgcat
      361 cagaaaggtc ccggaggtgc ttgcctccgg gtgagaagga actactgtgg tgggttgttc
      421 tgcaacgtta acatcaaacc gtcgcgacgc atcgctgcag cttcttccgg cttgtgcagt
      481 ctgtccagcg cgtcggcaag ccatgcgtaa tcgtaggcgt ccggacgttg tttcagcgcc
      541 gcgcggaagg cgagcgatgc ttcctgccat tctccgtgct tcatcagtga ctggcccagt
      601 gtgctccaca acagcgggcg atcgccgacg tttttgatct gctggcgcag cactttttcc
      661 agctgttccg gattgttggt tttcagtcga ggaatcggca gcagtaggcg atcgtcgtac
      721 tggcgtttca ggccatcgat gataatttgc tgggcagtat catgatcgtc acattcaata
      781 agatgttccg ccattgccac ctgcaacgct acctgatgac gcgttttccg gctttggttt
      841 ttccaccagt tacgcaaacc ttcactaccg ttatcggcac gcgcctgatc catcaggcca
      901 atccatgcct gttgttccag cattgcacga tgttcttcat caccaacatg ggctttcgcc
      961 attgatggga tgatatccag cagcgaactc catgcacctg tgcggatata cgcctgttcc
     1021 gccagacgta atacttccgg atggcgtggc gtaacttcca gcagcttatc cacgccgtgg
     1081 cgtgcagcat ggttttcatt acgggccagt tgcagacgta cacgggtgat ttctaccgga
     1141 atggtgtcat tgccggccag ctctgctgcg cgttccagat gttggttggc gcgtgcttca
     1201 tcaccacgtt gttgcgcggc ttcggcagcc agtagatagt tcaccaccgg ttgttccgcg
     1261 tgatcggcat ttttcgccat cagcttttca acttgctgat aatcgccttc cgccagtttc
     1321 agcagcgcct gttcggtctg cttacgggca cggcgacgct tacgtccgac aaaccaccca
     1381 cgggtgtgtg cgcctgtgcg gaagatccgc cgcagtagcc actcaatggc aaacagcact
     1441 accatcgcca gaatcaaaat aatcgccagg cccgtgacgc tggtttcgat attgtagttg
     1501 tcagtctgga tcagcacgta accctgatga ccggcaatca tcgggccaac cactatcccc
     1561 gcaatcagca aaacaaagag taataacact tttagcatgg ttattctcct tgcggcgcgg
     1621 ctgccggagt atcaccttgc ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtg
     1681 caggtgcagg tgcaggtgca ggtttagctt ccgttgtccc cgctgccggt tgtgccagca
     1741 ggttacgcac gcgggtctgc atcagttttt ccagcatcgc ctggctttgc agggtttccg
     1801 gaagatccat cgagatattt tgctggctta actggtccac ctcgtcgagg aacgctttgg
     1861 tggtggcatc atcagtatcg tagtaagctc gtacccaggt ggagacgttc tccagcgcct
     1921 ggcgataagt ctcttcctgg tgacgcggta cagcttgtgc tgcgaccagc aggcgagagc
     1981 gaatattttc gcgcagatag atatcctgat ttggcgctaa cagcggtacg gcggtgtcat
     2041 cacgacggcg aatcgtaatg aagttgtcca taaagttctg ccagcttttt tgcagattga
     2101 tacgccattc gctgatggaa ctggaaagct cttcaccgtc ggaatccatc ggcgaaccat
     2161 cgctgtcatt atcggccaga cgcaggttat ctacctgatt tgaaagctga ttaagcttaa
     2221 ggatgatgcc gtcataatcc acctgcgaaa ctgcagaaag gctggcgata tcatcggtaa
     2281 ttgcccgacg aacggtaatc agactcgggt cattcatatc cgccaggctg gcatctgcac
     2341 ttttcagcaa cgctgcagcg gtcgtgacgt cctgatcgct ccacagcttc cgtccggcga
     2401 gtttcaccag aaaatcggcc tgagccagca gccaggtttt agcatcgctg ccggaaatgg
     2461 tggcgacctt ttgttggact tcatccaact gttttgccag cgtttcttgc tgacgattcg
     2521 cctgcttaag ctgtgcagct tgttgcttaa taatgccttc cagctcggct ttttggctct
     2581 cctgggcttt ttgcaatgcc gtcagttggt tagccagggc atcgctggtg gcggtctgat
     2641 tgacggcctg ttgtttaccc cagccataca aaccgacacc cgccgccaga gcaatagcga
     2701 tagccaccgc gctgagaatc aatgcagtat tgttcttact ctttttttct gttgcgacag
     2761 gttgtgacgt ggtgtccacg gcctccctgg tctcttcaac cacggcggag gttttttctt
     2821 gttccgtcat tatggcttcc tgttatgaga gttattgtaa tgcccgtaaa agcgcatcgt
     2881 tgtcagcgtt atcggcgacc ttaatgtctt gccagcccag ttcccgggcg agtttcgcca
     2941 aacgctcact gacgaccaat agtcgacagt gtagtaacca gtgctcacga taccattgtg
     3001 ggatcagcga ccagagttgc tgcaacattt caccgctggt aacaacgacc atcgtcacct
     3061 cgcgggattg ccagcgcatc gcttcttctg caccatcgta atggattgcg catcgttgat
     3121 aacattcaca aaaagtgacc tcagcaccgc gcgctgtcag ggtatcccca attagctcac
     3181 gaccgccatt gccacgtaat atcagcgcac gtttgcccgc aatattttgt aattcaggta
     3241 attgtagcaa gacttcgctg atttcccgat cctgcgggta gagaatcttc tgtccgctta
     3301 cggtatgtag cgccagtgcg gtggtgcgtc caatggcgaa ataatcaggt agtcggggcc
     3361 atttacgatc ttgctgatgc agctgtgatt gggcaaaagc aaccgcgtgt tgcgagaggg
     3421 caaacaacag atcgctctcc cccagcgcag ccagttgatc agcaagttgc ggtaattgtc
     3481 gacccggaga aaactcaatc agcggaaaat gccaggccac ctgccccagt gtgcgcagac
     3541 ggctcactaa ctcttctcca gcgggagacg ggcgggtgac caggatactc atgccggggc
     3601 gtctccgtta tagacttcag cgaggatctc gcgcgcgccg ttattcagta gctcttctgc
     3661 cagcgaaatc cccatttgtt cggcattttg cggcgcaccg cggcgttcac cgcgaataat
     3721 ctgcgaaccg tccggcgcgc cgaccagcgc acgcagccag atttcaccat caataagctc
     3781 ggcgtagcta ccaattggca cctgacatcc accttcgaga cgggtattca tggcgcgttc
     3841 tgcggtaacg cgcagtgcag tttcgtggtg attcagcgcg gcaagcagct cgcgagtgcg
     3901 cgtatcatca aggcggcatt caataccgac tgcaccttgt cctaccgccg gaagagaaat
     3961 ctcgggtggt aacgcggcgc gaatgcggga ctccagacct aaacgtttta gtccggctac
     4021 ggcaagaatg atggcatcgt attcgccgtt atctagtttg ctcaggcgag tgccgacgtt
     4081 gccgcgcagg gagcggataa tcagatccgg gcggcgttca gccagttggc actggcgacg
     4141 taaactggac gtcccgacga tactgcctgc cggtaacgca tccagactgt cataattatt
     4201 ggacacaaag gcatcgcgag gatcttcacg ctcacaaata gtgaccaacc ccagaccttg
     4261 cgggaattca accggcacat ctttcattga atgtacggcg atatcggcgc gattctccag
     4321 gagcgcgact tccagctctt ttacaaataa gccttttccg cctactttcg ccagcggcgt
     4381 atcaagaatc acatcgccgc gcgtcaccat cggtaccagt tcaaccacca ggcccggatg
     4441 gctcgccatc aacttgtctt tgacatagtg tgcctgccag agtgcaagtg ggctttggcg
     4501 tgtggcaatt cttaaaacat tgtctaacat gcttgttacc gtcattatca tccgtggtcc
     4561 atcctaacat ccttgccaga gtgatgtcag tgttgtggtg aaacgtagac gcctgcgcaa
     4621 accgtaaaat gaggtctggc agtggatcct gacaggcgtt tcacgccgtc gtaataagga
     4681 atttacagag aataaacggt gctacacttg tatgtagcgc atctttcttt acggtcaatc
     4741 agcaaggtgt taaattgatc acgttttaga ccattttttc gtcgtgaaac taaaaaaacc
     4801 aggcgcgaaa agtggtaacg gttacctttg acatacgaaa tatcccgaat gccgcgtgtt
     4861 accgttgatg ttggcggaat cacagtcatg acgggtagca aatcaggcga tacgtcttgt
     4921 acctctatat tgagactctg aaacagagac tggatgccat aaatcaattg cgtgtggatc
     4981 gcgcgcttgc tgctatgggg cctgcattcc aacaggtcta cagtctactg ccgacattgt
     5041 tgcactatca ccatccgcta atgccgggtt accttgatgg taacgttccc aaaggcattt
     5101 gcctttacac gcctgatgaa actcaacgcc actacctgaa cgagcttgaa ctgtatcgtg
     5161 gaatgtcagt acaggaccca ccgaaaggtg agcttccaat tactggtgta tacaccatgg
     5221 gcagcacctc gtccgtaggg caaagttgtt cctctgacct ggatatctgg gtctgtcatc
     5281 aatcctggct cgatagcgaa gagcgccagt tactacaacg taaatgtagt ctgctggaaa
     5341 gctgggccgc ctcgctgggt gtggaagtca gcttcttcct gattgatgaa aaccgcttcc
     5401 gtcataatga aagcggcagc ctggggggcg aagattgtgg ctccacccag catatactgc
     5461 tgcttgacga attttatcgt accgccgtgc gtctcgccgg taagcgtatt ctgtggaata
     5521 tggtgccgtg cgacgaagaa gagcattacg acgactatgt gatgacgctt tacgcgcagg
     5581 gcgtgctgac gccaaatgaa tggctggatc tcggtggctt aagctcgctt tctgctgaag
     5641 agtactttgg tgccagcctt tggcagctct acaagagtat cgattcccca tacaaagcgg
     5701 tactgaaaac actgctgctg gaagcctatt cctgggaata cccgaaccca cgtctgctgg
     5761 cgaaagatat caaacagcgt ttgcacgacg gcgagattgt atcgtttggt ctcgatccat
     5821 actgcatgat gctggagcgt gttactgaat acctgacggc gattgaagat tttacccgtc
     5881 tggatttagt acgtcgctgc ttctatttaa aagtgtgcga aaagctcagc cgtgaacgcg
     5941 cctgcgtagg ctggcgtcgc gcagtgttga gccagttagt gagcgagtgg ggttgggacg
     6001 aagctcgtct ggcaatgctc gataaccgcg ctaactggaa gattgatcag gtgcgtgagg
     6061 cgcacaacga gttgctcgac gcgatgatgc agagctaccg taatctgatc cgctttgcgc
     6121 gtcgcaataa ccttagcgtc tccgccagtc cgcaggatat cggcgtgctg acgcgtaagc
     6181 tgtatgccgc gtttgaagca ttaccaggta aagtgacgct ggtaaacccg cagatttcac
     6241 ccgatctctc ggaaccgaat ctgaccttta tttatgtgcc gccgggccgt gctaaccgtt
     6301 caggttggta tctgtataac cgcgcgccaa atattgagtc gatcatcagc catcagccgc
     6361 tggaatataa ccgttacctg aacaaactgg tggcgtgggc atggtttaac ggcctgctga
     6421 cctcgcgcac ccgtttgtat attaaaggta acggcattgt cgatttgcct aagttgcagg
     6481 agatggtcgc cgacgtgtcg caccatttcc cgctgcgctt acctgcaccg acaccgaagg
     6541 cgctctacag cccgtgtgag atccgccatc tggcgattat cgttaacctg gaatatgacc
     6601 cgacagcggc gttccgcaat caggtggtgc attttgattt ccgtaagctg gatgtcttca
     6661 gctttggcga gaatcaaaat tgcctggtag gtagcgttga cctgctgtac cgcaactcgt
     6721 ggaacgaagt gcgtacgctg cacttcaacg gcgagcaatc gatgatcgaa gccctgaaaa
     6781 ctattctcgg caaaatgcat caggacgccg caccgccaga tagcgtggaa gtcttctgtt
     6841 atagccagca tctgcgcggg ttaatccgta ctcgcgtgca gcaactggtt tctgagtgta
     6901 ttgaactgcg cctttccagc acccgccagg aaaccgggcg tttcaaggcg ctgcgcgttt
     6961 ctggtcaaac ctgggggttg ttcttcgaac gcctgaatgt atcggtacaa aaactggaaa
     7021 acgccatcga gttttatggc gctatttcgc ataacaaact gcacggcttg tcagtgcagg
     7081 ttgaaaccaa tcacgtcaaa ttaccggcgg tggtggacgg ctttgccagc gaagggatca
     7141 tccagttctt cttcgaagaa acgcaagacg agaatggctt taatatctac attctcgacg
     7201 aaagcaaccg ggttgaggtg tatcaccact gcgaaggcag caaagaggag ctggtacgtg
     7261 acgtcagtcg cttctactcg tcatcgcacg atcgcttcac ctacggctca agctttatca
     7321 acttcaacct gccgcagttc tatcagattg tgaaggttga tggtcgtgaa caggtgattc
     7381 cgttccgcac aaaatctatc ggtaacatgc cgcccgccaa tcaggacaac gatacgccgc
     7441 tattacagca gtatttttcg tgatgagcgt gccggaaagc gaggcttatc cggcatgcaa
     7501 tcttagcgga aactgactgt ttcacccgct tgctgtgtcg ccgcctgttc cagcaaatcc
     7561 cagaaggttt cgccgctgcg atcgcaaatc cattcatcgc cttttaggtc aaaatggtag
     7621 ccgccctgtt tggttgccag ccacacctgg tgcagcggct cctggcggtt aataatgatt
     7681 ttgctgccgt tttcaaaggt aatggtcagt acgccgccgt tgatttcgca gtcgatatcg
     7741 ctgtcgccat cccagtcgtc caggcgttct tcaatggtca gccagagttg atcagccagg
     7801 cgatgaaatt cactgtcgtt cattgttgta tcctgtcttt aagtgatggc ggcagtatag
     7861 cggtatgggg tcagggcttc aaagtttgcc cgtctgttgc tgcgttccgg catgagtcat
     7921 ccgtcaacgg aataataatg tctctgttgt gaagttcacg cacattctct tctctgacgt
     7981 gtgttttcgc cgggcagggg gagggcgtaa tccttacctc tgtatcggtc catgtataaa
     8041 cattgcgatc gtcataagcg aagcgatcgt ttttagttag cggaccgtta actggaataa
     8101 tttgcacgct ctttgaccgt aattggcgga taaaataagc atgctgtttg ccgctattaa
     8161 atgagcgatc aatgaccagg tctggcgtat ccagcgggat tatctcaagc tggtcttcac
     8221 caaatgtcga taattttgtt acatacagcc gatctttata ttgcgcaata ttgccagtaa
     8281 tcagatggaa ttgttcaggt tcaatatctg gtaggtctat ccacttgctc cactgacggt
     8341 cggacgtgag ctgtttacgc cagcgaacgc gactttcccc tacttcaaaa gagacgtcat
     8401 catctacggg tatcgctttg ccagacaatt gcccaaaggg ataacgatgc atacccttat
     8461 tgtcgcgaaa aaccagcagt gaatgaggta tccagcgaat tatctcaaat gagtctgcat
     8521 tcgccgtaag gtgaacgcca tttaaataaa tattctgccc actgcggata agcagatcat
     8581 tggaatacaa atttctgttt acactcataa gacgtggatt aataccccac catctttttt
     8641 ctaatacagt aacattactg ctgctcccct gacgtcgacc ggacaaatag agatttttac
     8701 tatccaccag cgtggtagag ttaccttcga tagcttttag tgtcgccaga tcaacacgtg
     8761 acgcgccgct attactttca gtgcgctgtc cgtcaaaata gaggctatat ttatcgaccg
     8821 cgaagcggcc catagcctga aagctggcaa tatcgacagt gggtgttccc ggtggattgc
     8881 taacgatttt gccacgccag agaataacgc ggccgtctgt cacccagtgg tattcctcat
     8941 tttttatgct tatttcatcg gtgggtatat agccttccac caactcccgc tccccggata
     9001 tatttgtcac aaggcgcaaa tcggccctgc tcatctccgg aaactgggca atgattttct
     9061 tatcccccca ataaccttta ccgtaagaca taacaccgtg atcattgtaa tagtcgaacc
     9121 ctatggcctg tacagcaggc gtcagaaatg tgtgtcccaa caggatgagt gcaactgtga
     9181 ttatttgacc cttcattttg tcgcctcatt gcgcggggca ggaataaatt cgccgtcatc
     9241 agtaattttt ccgtccagat atcccgtttt gtaatttacg ccatagccat atcccgaatg
     9301 ttgttccttc cagattaagc gaacttgttt cggatcagcg gtgcggtatc gccagagctg
     9361 cgcgccatca aagctgtaaa tgtagcggtt atcgcgggca taccatctgc gcaaaaaggg
     9421 atggtcatta agatgggctt cgcgttcatc cggagaacgg ttatccagta agatcagcgg
     9481 tccgtcagat tcaaacacat gtaaaccgga atggtatggc cacccttcgg cacgcgtaag
     9541 taaatagccg tggcgtttcc cggcgttgaa acgtttgttg ataataagat tgggatcatc
     9601 aagtttcacg atttccagct tgcggtcacc agactctgtt tcgatgatgg tataaagcga
     9661 gtcctgatac tgggcgacag catcactgat gggttgaaac tgctccggat cgagtccggg
     9721 taattcttcc tgtttacaag caggtccttt acgccagaac actttctttt ccagcagaga
     9781 aaaatccaga cagtgctttg ttaaatcttc atcccactcc agattttcct tgtcgaggac
     9841 tttacgctgc agcccgtttt tatcacgcca ggtcaagagt gagccgggca tccagcgcac
     9901 gacggaaaag gtgtccgggt cggcatcaag ctgttcgccg ttgatcagga ttttggtccg
     9961 tgtacgagcc agggtatccc aggggccaaa tggcatagcc acacagggat taaacgcggt
    10021 agaaaacttt cctcgctgat cccatgactt ttgtgccagc acacggaaac tatcgggatc
    10081 tgcgaggcga tgcccattga tatacaagaa attagcgtca cgtaatatca gccccagtaa
    10141 gtcaggatcc cagggaggcc ggaattccac ctgatgcagt gttttgatat ctaagcgatt
    10201 tcccccgcca ttgttatcgg tgcgcttgcc ttcgaaatag agactgtgtt tatctgcggc
    10261 aaaatcaccc cacgccttga acgaggcgac atcgacggga ggtttgtctg gcgggttttg
    10321 cacaacttca cccgcgtaga ggatatagcg accatcgcta tgccaggagt aatgaggata
    10381 ccaggcctgt gcgccttcat agaggtagtc ggttgtcaga tcagtcagag gaggtacttt
    10441 atcccatttg tctagtgttg tcgggtctat taataaattg ggtaaacggc gcaaactttt
    10501 gtaattcacg tcaggaagag aaagatttgt ttctttataa gggcgcaggt ataccacgcc
    10561 ttttccctgt ttctggtaaa tattatgttc aggcggatca agccgacatc cccatgccga
    10621 cgataaaaac aatgaacaag acaacaaaca aaccacattg cgatagtgca taaagccatc
    10681 ctggcgcgag gtgccgatca cgaaactacc agcagaacat aattcctcgc acgtaagcgt
    10741 aacttttgcg gcaaatcgtc acttttcaga atctataaag gccatcgcta ttttctgata
    10801 gttgctgggg taaacttgag gttttcattc acgatataaa tacgagctac tgcgccatgg
    10861 tgtgactgat cacgttaaaa tgtgccggat tgacgccggg aagggtgtca acatgcgact
    10921 ctgcccaccg ttacctactt tattgtcttt cgcgtgttgt acttaggtcc caccaccggt
    10981 atctaacatt ccccacatta ttacgctttc tgccgaacgt gatttttata gaaatctatc
    11041 aattcatttt ccctgtacca taaagtttat ctttcacatc ctgccgaacg cgggtcttgt
    11101 accccccgtt gtgaagatct tcatacggag aaaaagaatc tttactccac cccagcagca
    11161 ctttatcgcc aggcataaaa tgcacatgca gaaaatcttc tccccattct cttttgggga
    11221 gaggtaacgt catattccgt ttctccagac gcattccagc attatactgt gtacgagtag
    11281 tacttaatgt ccagataacc tctgccgtat ccccactaac atcaccacaa caggttgttg
    11341 aaccgccacc atttgcaaaa gcattcgcgc ccgccacgcc gttaacactg aatcccagta
    11401 tcgggcggtc aatttcactg tggatttcca gaatcactcc acctcttggt cctccccata
    11461 ctgcctgtcc tatattccac aacacgacca gtaccggaac gacaattaac gtccgccata
    11521 cccagcgccc ccaccgggca taaccgcgcg taagcttttg gtcaaatttt tccagttggg
    11581 taaatagccc catcagattt tattccctct tatcaccagg ttggcaaagt tgtatgtcac
    11641 tggcgactaa ggtcagacaa caaaattgca tggtgcgcca ctcttttaaa agctccatgc
    11701 cgacgataag aacaacgaac aagccaacaa acaaactaca ttgcgatagt gcatagagcc
    11761 atcctggcgc gaggtgccga tcacgaaact accagcaaaa cataaatccc cacgagtaag
    11821 cgttatactc gcagcatttc ctcacttttc agacttcata aagagtcgct aaacgcttgc
    11881 ttttacgtct tctcctgcga tgatagaaag cagaaagcga tgaactttac aggcaatcca
    11941 taatgaaaaa cgtgtttaag gcactcactg tattaattac tctcttcagc ctgacgggct
    12001 gcggtctgaa aggtccgctc tatttcccgc ctgcagataa aaacgcaccg ccgccgacca
    12061 aaccggtaga gacgcaaacg caatccacgg tgccggataa aaacgatcgc gccactggcg
    12121 atggtccatc ccaggtgaat tactaaaagt cagtttctgt acccgcgtga ttggagtaaa
    12181 tgatgcagtt ctcgaaaatg catggccttg gcaacgattt tatggtcgtc gacgcggtaa
    12241 cgcagaatgt ctttttttca ccggagctga ttcgtcgcct ggctgatcgg cacctggggg
    12301 tagggtttga ccaactgctg gtggttgagc cgccgtatga tcctgaactg gattttcact
    12361 atcgcatttt caatgctgat ggcagtgaag tggcgcagtg cggcaacggt gcgcgctgct
    12421 ttgcccgttt tgtgcgtctg aaaggactga ccaataagcg tgatatccgc gtcagcaccg
    12481 ccaacgggcg gatggttctg accgtcaccg atgatgatct ggtccgcgta aatatgggcg
    12541 aacccaactt cgaaccttcc gccgtgccgt ttcgcgctaa caaagcggaa aagacctata
    12601 ttatgcgcgc cgccgagcag acaatcttat gcggcgtggt gtcgatggga aatccgcatt
    12661 gcgtgattca ggtcgatgat gtcgataccg cggcggtaga aacgcttggt cctgttctgg
    12721 aaagccacga gcgttttccg gagcgcgcca atatcggttt tatgcaagtg gttaagcgcg
    12781 agcatattcg tttacgcgtt tatgagcgtg gggcaggaga aacccaggcc tgcggcagcg
    12841 gcgcgtgtgc ggcggttgca gtagggattc agcaaggttt gctggccgaa gaagtacgcg
    12901 tggaactccc cggcggtcgt cttgatatcg cctggaaagg tccgggtcac ccgttatata
    12961 tgactggccc ggcggtacat gtctacgacg gatttattca tctatgaagc aaccagggga
    13021 agaactgcag gaaacactca cggagcttga tgaccgggcg gttgtcgatt atctgattaa
    13081 aaatcctgag ttttttatcc gtaatgcgcg cgcagtagaa gcgatacgtg tgccgcatcc
    13141 ggtacgcggc accgtttcgt tggtcgagtg gcacatggcc cgcgcacgta atcatattca
    13201 tgttctggaa gagaacatgg cgctgttgat ggaacaggct atcgccaacg aaggcctgtt
    13261 ttatcgccta ctctacctgc agcgcagtct caccgccgcc agcagtctcg acgatatgct
    13321 gatgcgcttt caccgctggg cgcgcgatct cggcctggca ggtgcgagtc tgcgcctgtt
    13381 tccggatcgc tggcgcttag gtgcgccgtc gaaccacact catctggcat taagccgtca
    13441 gtctttcgaa ccgctgcgta ttcagcgttt ggggcaggaa cagcactatc ttgggccgct
    13501 taacggacca gagctgctgg tggtgctacc ggaagcgaaa gcggtgggat cggtggcgat
    13561 gtcgatgctg ggaagcgatg ctgatttggg tgtcgtgctg tttaccagtc gcgatgccag
    13621 tcactatcaa caagggcaag gaacgcagtt acttcatgaa attgcgctga tgttgccgga
    13681 gcttctggag cgttggattg aacgcgtatg accgatttac acaccgatgt agaacgctac
    13741 ctacgttatc tgagcgtgga gcgccagctt agcccgataa ccctgcttaa ctaccagcgt
    13801 cagcttgagg cgatcatcaa ttttgccagc gaaaacggcc tgcaaagctg gcagcaatgt
    13861 gatgtgacga tggtgcgcaa ttttgctgta cgcagtcgcc gtaaagggct gggagcagca
    13921 agtctggcgt tacggctttc tgcgctacgt agcttttttg actggctggt cagccagaac
    13981 gaactcaaag ctaacccggc gaaaggtgtt tcggcaccga aagcgccgcg tcatctgccg
    14041 aaaaacatcg acgtcgacga tatgaatcgg ctgctggata ttgatatcaa cgatcccctc
    14101 gctgtacgcg accgtgcaat gctggaagtg atgtacggcg cgggtctgcg tctttctgag
    14161 ctggtggggc tggatattaa acacctcgac ctggagtctg gtgaagtgtg ggttatgggg
    14221 aaaggcagca aagagcgccg cctgccgatt ggtcgcaacg ctgtggcgtg gattgagcac
    14281 tggcttgatt tgcgcgacct gtttggtagc gaagacgacg cgctttttct gtcgaaactg
    14341 ggcaagcgta tctccgcgcg taatgtgcag aaacgctttg ccgaatgggg cataaaacaa
    14401 gggctgaata atcacgttca cccacataaa ttacgtcact cgttcgctac gcatatgctg
    14461 gagtcgagcg gcgatcttcg tggtgtgcag gagctactgg gtcatgccaa cctctccacc
    14521 acgcaaatct atactcatct tgattttcaa caccttgcct cggtgtacga tgcggcgcat
    14581 ccacgcgcca aacgggggaa ataatgcgtt tttaccggcc tttggggcgc atctcggcgc
    14641 tcacctttga cctggatgat accctttacg ataaccgtcc ggtgattttg cgcaccgagc
    14701 gagaggcgct tacctttgtg caaaattatc atccggcgct gcgcagcttc cagaatgaag
    14761 atctgcaacg cctgcgccag gcggtacggg aagtggaacc cgagatttat cacgacgtga
    14821 cgcgctggcg ttttcgttcg attgaacaag cgatgctcga cgccgggctg agtgccgaag
    14881 aagccagtgc aggcgcacac gcagcaatga tcaactttgc caaatggcgc agccgaatcg
    14941 acgtcccgca gcaaactcac gacaccttaa aacagctggc gaagaaatgg ccgctggtgg
    15001 cgatcaccaa cggtaacgcc cagccggaac tgtttggttt gggggattat tttgagtttg
    15061 tgctgcgcgc tggcccgcac gggcgctcaa aaccgttcag cgatatgtac tttctggctg
    15121 cggaaaaact caacgtgccg atcggcgaga tcttacatgt tggggacgat ctcaccactg
    15181 acgtgggtgg ggcaattcgc agcggaatgc aggcctgttg gatcagaccg gaaaatggcg
    15241 atctgatgca aacctgggac agccgtttac tgccgcatct ggaaatttcc cggttggcat
    15301 ctctgacctc gctgatataa tcagcaaatc tgtatatata cccagctttt tggcggaggg
    15361 cgttgcgctt ctccgcccaa cctattttta cgcggcggtg ccaatggacg tttcttacct
    15421 gctcgacagc cttaatgaca aacagcgcga agcggtggcc gcgccacgca gcaaccttct
    15481 ggtgctggcg ggcgcgggca gtggtaagac gcgcgtactg gtgcatcgta tcgcctggct
    15541 gatgagcgtg gaaaactgct cgccatactc gattatggcg gtgacgttta ccaacaaagc
    15601 ggcggcggag atgcgtcatc gtatcgggca gctgatgggc accagccagg gcggcatgtg
    15661 ggtcggcacc ttccacgggc tggcgcaccg tctgctgcgt gcgcaccata tggacgccaa
    15721 tctgccgcag gattttcaga tcctcgacag cgaggaccag ctacgcctgc tcaaacgcct
    15781 aatcaaggcg atgaacctcg acgagaagca gtggccgccg cgccaggcaa tgtggtacat
    15841 caacagccag aaagatgaag gcctgcgtcc acatcatatt caaagctacg gtaatccggt
    15901 ggagcagacc tggcagaagg tgtatcaggc gtatcaggaa gcgtgtgacc gcgcgggcct
    15961 ggtggacttc gccgagctgc tgctacgtgc tcacgagttg tggcttaaca agccgcatat
    16021 ccttcagcac tatcgcgaac ggtttaccaa tatcctggtg gacgaattcc aggataccaa
    16081 caacattcag tatgcgtgga tccgcctgct ggcgggcgat accggcaaag tgatgatcgt
    16141 tggcgatgac gatcagtcaa tctacggctg gcgtggagcg caggtggaga atattcagcg
    16201 tttcctcaat gatttccccg gtgctgaaac tatccgtctg gagcagaact accgctctac
    16261 cagcaatatt ctgagcgccg cgaacgccct gattgaaaac aacaacgggc gtttgggtaa
    16321 aaaactgtgg accgatggcg cggacggtga gcctatttcc ctctattgcg cttttaacga
    16381 actcgatgaa gcgcgttttg tggttaaccg catcaaaacc tggcaggaca acggcggggc
    16441 gcttgccgag tgcgccattc tctaccgcag taacgcccag tcgcgtgtgc tggaagaagc
    16501 actgttgcag gcgagtatgc cgtaccgtat ttacggcggg atgcgcttct tcgaacgcca
    16561 ggaaatcaaa gatgcgctct cgtatctgcg cctgattgcc aaccgtaacg acgacgcggc
    16621 ctttgagcgt gtggtgaata cgccaacgcg gggtattggt gaccggacgc tggacgtggt
    16681 acgtcagaca tcgcgcgatc gccagttaac actctggcag gcatgtcgtg aactgttgca
    16741 ggaaaaagcc ctcgccggac gtgctgccag cgccttacag cggtttatgg aactgatcga
    16801 cgccttagcg caggaaactg ccgatatgcc gctgcatgta cagactgacc gggtaattaa
    16861 agactccggc ctgcgtacca tgtacgagca ggagaagggc gaaaaaggtc agacgcgtat
    16921 cgaaaactta gaggaactgg tgacggcaac gcgccagttc agctacaacg aagaagacga
    16981 agatttaatg ccgctgcagg cgttcctatc ccatgcggcg ctggaagcag gcgaagggca
    17041 ggcggatacc tggcaagatg cggtacaact gatgaccatg cactcggcga aaggtctgga
    17101 gttcccgcag gtgtttatcg tcggtatgga agagggcatg ttcccaagcc agatgtcgct
    17161 ggatgaaggc gggcgtctgg aagaagaacg ccgtctggcc tacgttggcg taacccgcgc
    17221 gatgcagaaa ctgacgctga cctacgcgga aactcgccgt ctgtatggca aagaggttta
    17281 ccatcgcccg tcgcgcttta ttggcgagct gccggaagag tgtgtggaag aggtgcgcct
    17341 gcgcgccacg gtaagccgcc cggtcagcca tcagcggatg ggtacgccga tggtcgagaa
    17401 cgacagcggc tacaagctcg gccagcgcgt acgccacgct aagtttggtg aaggcaccat
    17461 tgtcaatatg gaaggcagcg gtgagcatag ccgtttgcag gtggcatttc agggccaggg
    17521 tattaaatgg ctggtggcgg cttatgcccg gctggagtcg gtgtaacgtt gccggatgcg
    17581 gtgctgcgca ccttacttgg cctaaaaaat cattcagatt caataaattg caacgtcatg
    17641 taggccggat agggcgttta cgccgcatcc ggcatctgcg ccatcttcag tatctgacac
    17701 aaaactatcg ttttaacctt tccgctctac ggaaatcatg gtgacaaaag gatagcgttg
    17761 ccacgggatt gccccgcctt tcatatacat atgtgaaatc gtgccatcaa gataaagcag
    17821 ctgctcaacg ttcagtttcg ctttggcata acaggcaaaa tcataaaaat ttgtcgcctg
    17881 ctggctcaac aaaaacacgg cattcccatg tttattaatc ccaacaccgt tacgaatttt
    17941 acgtgaggcg acgttgggat gaatacgcgg attaattaca ctgttttcca tcaacattgg
    18001 ccctgactgc accgcaaact gaatctcttt actggttttg aaggcatcca gacgaacgat
    18061 gccgacttta tctcccgcga cataaaacac gccgccagga cggataaaga aattcccttc
    18121 acctgaagcg agatttaacg ccaccttctg ctgaccgttt tcgatgtaca aaccgagcgg
    18181 cgcatagctt tcatcataga tgccgccgtt catcgccatc tgcacctgac cctgactatt
    18241 catatccgcc agcagagcat gtaaagttcc ccacgcttcg ccattggctt tttgccagta
    18301 cattttcacc cgctctgttt gaggattaac ggtatacgcc tgtacggtca gcgtcggatc
    18361 tgagagtgcg caatcatcag cggcaacagc aaacaaggga agtaaggtga gggtgagaaa
    18421 aatccgtttg agattcaagg tgatcattcc tttaccaatg agtagccgat gcgccattat
    18481 aggtcctgga tgttggattt ttttatcctg ttagcgacct tgacgagtac caaaaagcgc
    18541 gaagttcaac tattgttctg tggtgttctg ttgcgtgttg acggcaaaat tttgctggcg
    18601 taacatgcgc gcacgatcac tctaagagga cattcgcctt ggacacaccc agtagatact
    18661 ggctcactat cctgtcatcc aggatcaact cctaaggcta tccctttttg ctgatagcct
    18721 tagcggttgt cagcgacctc aatttttccc gtcgcgctga gtcaggctgt ttaatggtct
    18781 gaaacccaat ttgtttctgt gtgcccaccg aactgtccga tattttaagc attgggagtc
    18841 ccggtcatgc tgagcgcatt tcaactggaa aataaccgac tgacccggct ggaagtcgaa
    18901 gagtcacaac cccttgtaaa tgcagtatgg attgatcttg tcgaaccgga cgacgacgag
    18961 cgactgcgcg tacaatctga acttggccag agcctggcaa cccgcccgga actggaagac
    19021 atcgaagcat cggcacgttt ctttgaagac gacgacggcc tgcatattca ctccttcttc
    19081 ttctttgaag atgcggaaga tcacgccggt aactccactg tggcatttac catccgtgat
    19141 ggtcgtctgt ttactctgcg tgagcgtgaa ctgcccgctt ttcgtctgta tcgtatgcgt
    19201 gcccgtagcc agtcgatggt agacggtaac gcctacgagt tgctgctgga tctgttcgaa
    19261 accaaaatcg aacagttggc agatgaaatt gaaaatatct atagcgacct ggagcagttg
    19321 agccgggtga ttatggaagg gcatcagggc gatgagtacg acgaggcgct ctccacgctg
    19381 gcggaactgg aagatatcgg ctggaaagtt cgcctgtgtc tgatggatac ccagcgcgcg
    19441 ctcaacttcc tggtacgcaa ggcgcgttta ccgggtgggc aactggagca ggcgcgtgaa
    19501 atcctgcgag atatcgaatc cctgctgccg cataacgaat ccctgttcca gaaggtgaac
    19561 ttcctgatgc aggcggcgat gggctttatc aacatcgagc agaaccgcat catcaaaatc
    19621 ttctcggtgg tatccgtggt gttcctgcca ccgacgctgg tggcttccag ctacgggatg
    19681 aactttgaat ttatgccaga gttgaagtgg agtttcggct acccaggcgc gattatcttt
    19741 atgatcctcg cgggcctggc accgtatctg tactttaagc ggaagaactg gttgtaatat
    19801 gagtgccgga taaagctatt tttttatccg gcttatttct aaaaattgtc ttttttaaag
    19861 tcatgcggat tgaaaactcg ctctatttta ttttcttgtg ttaattgata ctcagtaaaa
    19921 tcgttaatac caatatgagt gttacgataa gggtattcat taataaccat taagtaattt
    19981 ttcacgatgc aattatcatt gctcttaatt gtcgggaaac agtagagatc ttcatacggc
    20041 ccttcgatat atttttttat agtcagtgaa gccattaccg atatcaatga cataccaggt
    20101 atgccaacta ccttctggta ggggatgttg atttttaatt tcattctgat gcttcagata
    20161 ccaatttatt ttatcgctgt cagtccatgg cagatgatca acgaccagaa agactgaatt
    20221 gccagcatca gagtatgtat acaaaatctg tacaggacgc aatgtccata aattatagat
    20281 gcagcataga agaaataaac ataatataat tttctggagt ttgcttttca tagataattt
    20341 tcgattcttt cgatagtgtt aaaattggtg aaaaaaggtt tgaaagcaaa gtctttatgc
    20401 cgttgtaaaa agaaccatat gcgaaaaaat ctatatttgc tgtaagtctt gtttttgata
    20461 tcagttacat caagaccgaa gtggtcctgt gctacaaaat gaattgttgc actccatcct
    20521 atagcgtatt tttgaaaact taaaagagat attttttgag ctgaaatatc atgaacactg
    20581 attcctaagc cattaaaact gtcctcgaag tcattaaatt tatttagtct tgatgaaagt
    20641 aatttggttt taatgctttg caggataaga ggttggtatc ctgtcgaggg agttgaatta
    20701 atgccatttt cgatacatt
//
LOCUS       DAZGOB010000083        20423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGOB010000083 DAZGOB010000000
VERSION     DAZGOB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.083"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(156..902)
                     /gene="cutC"
                     /locus_tag="ACHLIF_003242"
     CDS             complement(156..902)
                     /gene="cutC"
                     /locus_tag="ACHLIF_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO9607208.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(916..1482)
                     /gene="yecM"
                     /locus_tag="ACHLIF_003243"
     CDS             complement(916..1482)
                     /gene="yecM"
                     /locus_tag="ACHLIF_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9607209.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1698..3431
                     /gene="argS"
                     /locus_tag="ACHLIF_003244"
     CDS             1698..3431
                     /gene="argS"
                     /locus_tag="ACHLIF_003244"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO9607210.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLDKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3473..3517)
                     /gene="yecV"
                     /locus_tag="ACHLIF_003245"
     CDS             complement(3473..3517)
                     /gene="yecV"
                     /locus_tag="ACHLIF_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO9607211.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3608..4096
                     /gene="yecT"
                     /locus_tag="ACHLIF_003246"
     CDS             3608..4096
                     /gene="yecT"
                     /locus_tag="ACHLIF_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO9607212.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4216..4608)
                     /gene="flhe"
                     /locus_tag="ACHLIF_003247"
     CDS             complement(4216..4608)
                     /gene="flhe"
                     /locus_tag="ACHLIF_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO9607213.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4608..6686)
                     /gene="flhA"
                     /locus_tag="ACHLIF_003248"
     CDS             complement(4608..6686)
                     /gene="flhA"
                     /locus_tag="ACHLIF_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO9607214.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMELQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHAPRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6679..7827)
                     /gene="flhB"
                     /locus_tag="ACHLIF_003249"
     CDS             complement(6679..7827)
                     /gene="flhB"
                     /locus_tag="ACHLIF_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO9607215.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8029..8673)
                     /gene="cheZ"
                     /locus_tag="ACHLIF_003250"
     CDS             complement(8029..8673)
                     /gene="cheZ"
                     /locus_tag="ACHLIF_003250"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO9607216.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8684..9073)
                     /gene="cheY"
                     /locus_tag="ACHLIF_003251"
     CDS             complement(8684..9073)
                     /gene="cheY"
                     /locus_tag="ACHLIF_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO9607217.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9088..10137)
                     /gene="cheB"
                     /locus_tag="ACHLIF_003252"
     CDS             complement(9088..10137)
                     /gene="cheB"
                     /locus_tag="ACHLIF_003252"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO9607218.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10140..11000)
                     /gene="cheR"
                     /locus_tag="ACHLIF_003253"
     CDS             complement(10140..11000)
                     /gene="cheR"
                     /locus_tag="ACHLIF_003253"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO9607219.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRCFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11019..12623)
                     /gene="tap"
                     /locus_tag="ACHLIF_003254"
     CDS             complement(11019..12623)
                     /gene="tap"
                     /locus_tag="ACHLIF_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO9607220.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNNDLEHQATWLESNQLSDFLTAPVQESQNAF
                     DVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQP
                     LAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHI
                     GIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTA
                     QAGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVD
                     DIMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSR
                     VAVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12669..14330)
                     /gene="tar"
                     /locus_tag="ACHLIF_003255"
     CDS             complement(12669..14330)
                     /gene="tar"
                     /locus_tag="ACHLIF_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO9607221.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQAPNWETF"
     gene            complement(14475..14978)
                     /gene="cheW"
                     /locus_tag="ACHLIF_003256"
     CDS             complement(14475..14978)
                     /gene="cheW"
                     /locus_tag="ACHLIF_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO9607222.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14999..16963)
                     /gene="cheA"
                     /locus_tag="ACHLIF_003257"
     CDS             complement(14999..16963)
                     /gene="cheA"
                     /locus_tag="ACHLIF_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO9607223.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16968..17894)
                     /gene="motB"
                     /locus_tag="ACHLIF_003258"
     CDS             complement(16968..17894)
                     /gene="motB"
                     /locus_tag="ACHLIF_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO9607224.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17891..18778)
                     /gene="motA"
                     /locus_tag="ACHLIF_003259"
     CDS             complement(17891..18778)
                     /gene="motA"
                     /locus_tag="ACHLIF_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO9607225.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQMTTEEA"
     gene            complement(18905..19483)
                     /gene="flhC"
                     /locus_tag="ACHLIF_003260"
     CDS             complement(18905..19483)
                     /gene="flhC"
                     /locus_tag="ACHLIF_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO9607226.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19486..19836)
                     /gene="flhD"
                     /locus_tag="ACHLIF_003261"
     CDS             complement(19486..19836)
                     /gene="flhD"
                     /locus_tag="ACHLIF_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO9607227.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4923 a         5601 c         4955 g         4944 t
ORIGIN      
        1 ttagcacaac gctatgttca ctgtcacccg ctcaacagat cacggttatc gttcgttttt
       61 tatactgctc agggataaaa aaaggcccct gttgaaattg caggggcctg gtacgagcaa
      121 gcatcatatt gggcgacatg atgcaacggt aaaaatcatt tggcctgatg gcgttcaatg
      181 attcctttca tttcagcaac cgccgccccg tctacgatat aacgcgaata ctcgtccgcg
      241 tgttcatctg atgacatgga caatccttga ttacgataac gcatcggtga ggcttgccac
      301 gctcccgcgg agctatggac ttccagcact ccggcatcga ggaagtggtg caggttttct
      361 gcacggactc ctgctccggc cataatgatt ggagcatcac gatgggcaat aagttccata
      421 atttttgata aaccttgcag tgcgtctgat ttttgccctg atgtcagtac ccgggcaatg
      481 cccaattccg caagattatt gagtgtatat aaagggttag cgcacatatc gaaggcgcga
      541 tgaaaagtca ctgccagcgg accggcagca gccattattt tttccattcg tggcatatcg
      601 acattcccgt caacatcgag aacgcccgtc accagtccag gaaaacctaa ttcgcggacc
      661 gtgcgcacat cctcaagaat ggcggcaaac tcaccgtcgc tgtagcaaaa atcaccaccg
      721 cgtgggcgaa ttatcggatg cacagggatc gtcacccgct ggcgcacgga tttcagtacc
      781 cccaacgacg gcgttaagcc cccctctttt ggggctgcgc ataattcaac tctgtctgcg
      841 ccgttttgct gcgccgttag tgcacattcc atgctgtaac agcaaatttc cagtaatgcc
      901 atttttgctc cttaattacg ctgactgctc gctggcaacg atctcttcaa tggaccacgg
      961 atgaaactta atagttgttt tgccatcggt gaccgccagc gtcggattcg gtaatcgctc
     1021 atgctcacct tttggtgaac tggttttaac tgagatcccc ggtaggctga gtccttcatc
     1081 agaaagcaat gccagcgcac gggcattcag cgtttctgga tcacccggca gaacgatttc
     1141 aatatgttcc caaccttcgt gtgggtaacg tttttccccg ggccacggta gctccacaat
     1201 agaaaactgc cagtgcgcaa cctgtaccgg ttcatgcaat ttaaacagac aaatcggtct
     1261 gccattgatc atattttctg acaaaagctc gccacactgt tcaaacccgc gacgccagcg
     1321 ttcagcagtg gcgttttgat ggcaacgcaa agaaatgtga tcggcagtca gcggagtgat
     1381 attcaacccc agacggaggg aaagttcatc taatgcgtgg gtaaatcgcg gtaaatccga
     1441 tgcaatatcc tgcagctcgt cgatagattg ccagttcgcc ataatcactc ttcgtctttc
     1501 agtaaaagcg ttaatttacc ctgttgccct gcgccaacca accgctgatt tcacgccgct
     1561 tctgatgcaa tagtgaaaac ggcaatacgc cacgcgcacg ttgctgacga aaacagccat
     1621 ttgcagtata ctcccgccct aatttcttta actggtgcgg gcaatttttg ctcgcttcat
     1681 caatgtaagg tattccggtg aatattcagg ctcttctctc agaaaaagtc cgtcaggcca
     1741 tgattgcggc aggcgcgcct gcggattgcg aaccgcaggt tcgtcagtca gcaaaagttc
     1801 agttcggcga ctatcaggct aacggcatga tggcagttgc taaaaaactg ggtatggcac
     1861 cgcgacaatt agcagagcag gtgctgactc atctggatct taacggtatc gccagcaaag
     1921 ttgagatcgc cggtccaggc tttatcaaca ttttccttga tccggcattc ctggctgaac
     1981 atgttcagca ggcgctggcg tccgatcgtc tcggtgtttc tacgccagaa aaacagacca
     2041 ttgtggttga ctactctgcg ccaaacgtgg cgaaagagat gcatgtcggt cacctgcgct
     2101 ctaccattat tggtgacgca gcagtgcgta ctctggagtt cctcggtcac aaagtgattc
     2161 gcgcaaacca cgtcggcgac tggggcactc agttcggtat gctgattgca tggctggaaa
     2221 agcagcagca ggaaaacgcc ggtgaaatgg agctggctga ccttgaaggt ttctaccgcg
     2281 atgcgaaaaa gcattacgac gaagatgaag agttcgccga gcgtgcacgt aactacgtgg
     2341 taaaactgca aagcggtgac gaatatttcc gcgagatgtg gcgcaaactg gtcgacatca
     2401 ccatgacgca gaaccagatc acttacgatc gtctcaacgt gacgctgacc cgtgatgacg
     2461 tgatgggcga aagcctctac aacccaatgc tgccaggaat tgtggcggat ctcaaagcca
     2521 aaggtctggc agtagaaagc gaaggggcga ccgtcgtatt ccttgatgag tttaaaaaca
     2581 aggaaggcga accgatgggc gtgatcattc agaagaaaga tggcggctat ctctacacca
     2641 ccactgatat cgcctgtgcg aaatatcgtt atgaatcact gcatgccgat cgcgtgctgt
     2701 attacatcga ctcccgtcag catcaacacc taatgcaggc atgggcgatc gtccgtaaag
     2761 caggctatgt accggaatcc gtaccgctgg aacaccacat gttcggcatg atgctggata
     2821 aagacggcaa accgttcaaa acccgcgcgg gtggtacagt gaaactggcc gatctgctgg
     2881 atgaagccct ggaacgtgca cgccgtctgg tggcagaaaa gaacccggat atgccagctg
     2941 acgagctgga aaaactggct aacgcggttg gtattggtgc ggtgaaatat gcggatctct
     3001 ccaaaaaccg caccacggac tacatcttcg actgggacaa catgctggcg tttgagggta
     3061 ataccgcgcc atacatgcag tatgcttaca cgcgtgtatt gtccgtgttc cgtaaggcag
     3121 aaattaacga agagcaactg gctgcagctc cggtaatcat ccgtgaagat cgtgaagcgc
     3181 aactggcagc tcgcctgctg cagtttgaag aaaccctcac cgtggttgcc cgtgaaggca
     3241 cgccgcatgt gatgtgtgct tacctgtacg atctggctgg tctgttctct ggcttctacg
     3301 agcactgccc gatcctcagc gcagaaaacg aagaagtgcg taacagccgt ctgaaactgg
     3361 cacaactgac agcgaagacg ctaaagctgg gtctggatac gctgggtatt gagacagtag
     3421 agcgtatgta atcgattttt cgtgagagta aagcctgatc agggttacct gatcaggctt
     3481 ttttattgcc atctaaatgt attctgaaaa tggacatgcc attgttttct gactgttgga
     3541 taagaggcca gaagcgtaat atccggcccc agggaaacga taacggttga atttaaggaa
     3601 taccgcagtg tttaaatttc ttgtattaac attaggcatt atctcttgcc aggcttacgc
     3661 agaagatacg gttatagtaa acgaccatga catttcagcc atcaaagatt gttggcaaaa
     3721 aaattcagat gatgatactg acattaacgt gatcaaatca tgcctgcgac aagaatacaa
     3781 tctcgtcgat gcgcaattaa ataaagccta tggtgaagct tatcgttata tagaacaagt
     3841 gccacgcaca ggtgtaaaaa aacctgatac cgaacaactt aacttgctta aaaaatcaca
     3901 gcgagcctgg ctggatttta gggacaaaga atgtgaatta atcctttcaa atgaggacgt
     3961 tcaggattta agtgaccctt attctgaatc agaatggctc tcatgtatga tcatacagac
     4021 caatacgcga actcgccagt tgcagctata ccgtaactct gaagattttt atccaagccc
     4081 tttgacaaga ggataattca catctttttg gcatgttttg ttgcaagcta ttcctgataa
     4141 ataattgcaa caagacatcg agcctttttc actgagttat taaacatact cgcgagcgcg
     4201 taattttttt gtccttcagc gataattcac aatcacttca ttacgttgta cctttagcgg
     4261 tggaattaac cgaccaccgc ctggcacttc ccagataaat cgcaacggtt ctgctgccgc
     4321 tataccggaa aaggccacgg tggttccgct ctgcccctct aattcgacac aacgagattg
     4381 cgaacacaag cgaacccgca gtcctgaagg tgtcgggccg ataagctgat aacgccacgc
     4441 taccagcgtc atcaatcctg aagcaggttg tcgcgtagaa agaggcgcag acgacatcga
     4501 ctcaccgcga tgatttagcg taattcctac actacttgcc tgccacatcc cctccccggc
     4561 ggcttgcacc agcagcggaa acaataatat tgctaataag gttctcatta tttgccgcca
     4621 attgtcgccg tcatgcggat atgtcggtta tcagacagtt ccagattcga caggaccact
     4681 aactgcggca agctgcggcg caggaagcga gacaataatg gtcgcggcgc gtggttcacc
     4741 aacaatactg gcggcgcacc cagcatctcc tgacgggata gcgcttcctg agtttgcgcc
     4801 agtaaacgat ccgccagccc tggctccagt cctcccccgc cctgcagcgc ctgtagtaac
     4861 aaacgttcca gcggtgtatc gaggccaata acatggactt catctttgcc aggaaaccac
     4921 tgctgggtaa tcgcccgtcc caacgccacg cgcacgacgg cggttaattc atgtggatcg
     4981 ctttggatgg gcgcatgttc cgccagcgtt tcgagaatgg tgcgcatatc gcgaatcggt
     5041 actttttcat cgaggagatt ttgcagcact ttatgcagtg tggtgagcgt gacgacgcca
     5101 ggaacgagat cttccgtcag ctttggcatc tcctgggcga cgcgatccaa cagctgttgc
     5161 gcctcctgac gaccaaacag ctctgcggca tgctggctaa tgaggtggtt aagatgcgtt
     5221 gctaccaccg tgctggcctc aaccactgtg tacccctgaa tctgcgcctg ttcttttagc
     5281 gcactttcaa tccagatagc attcaggcca aatgccggat cgacggtcgc ctcaccaggt
     5341 aacgtcccgg cagcggttcc agggttaatc gccagccagc gccccggata agcatcacca
     5401 ctgccaatct ccacgccttt catcaaaatg cgatagcggg caggttgcag ctccatattg
     5461 tcgcgaatgt gcaccactgg cggcagaaat cccatctcct gggcaaattt cttgcggata
     5521 ctgcgtatac ggcccaacaa ctcaccatcc tgctggaaat cgaccatcgg gatcagtcga
     5581 taacccactt ccattcccag agaatcttcc agttgtacat cgttccacgt cgcttcgaca
     5641 acggtattat tctctgccat ttttaccggt ttgggttcga caggcgcttt ttgttcgcgt
     5701 ccgcgtatcc accaggccag ccccagcaat ccggcagtga acagcaaaaa taccaggttc
     5761 ggcattccag gcaccaggcc gagtaaaccg agcacggcgg cgcttaacaa cataacgctt
     5821 gggttactga aaagctgatt caccatctgc tcgccaacat cctgatcggt gctgacacgc
     5881 gtaacgatga cccccgcggc ggtagaaatc accagcgccg gaatttgtgc caccagaccg
     5941 tcgccaatgg tcaatagcgt ataactttcc gccgcgtgtc ccatgctcat gccatgttgc
     6001 agcacgccga ccagcaaccc gccgacaacg ttaatgacca tgatgaggat cccggcgatg
     6061 gcatcgccgc gaacaaactt acttgccccg tccattgagc cgtaaaaatc ggcttcctga
     6121 gtcacttcgg agcggcgttt tttcgcctca tcttcaccaa tcaatccagc gttaaggtcg
     6181 gcgtcaatcg ccatctgctt acccggcata ccatcgagaa caaagcgcgc acccacttct
     6241 gcgatacgcc cggcaccttt ggtaatgacc ataaagttga tgatcacgag aatgacaaac
     6301 actacgatac cgatagcgaa attgccacca acgaggaagt gaccgaatgc ttcgaccacc
     6361 ttcccagccg ccgccgcgcc ggtatgccct tccatcaaaa tgatacgggt tgaagccacg
     6421 ttaagtgcca gacgcaacaa cgtagtaaac aacagaatgg tcggaaacgc agcaaactca
     6481 agcgtgcgct gggtaaacat cgccaccagc aacaccatga tcgacaaggc aatattgaag
     6541 gtaaacaaca ggtcgagtat gaatgcgggc agtggcagca ccatcatcga caagatcaac
     6601 aggatcaaaa tcggtccggc aaggatctgc cattgtgtcg atttcaggtt tgcgggcagg
     6661 cgcagcatcg cggccagatt actcatgggt cggtttctcg ttaataaaat ccagggcttc
     6721 cggcaccgga agatgagtag gttgtacagg gcgctgtcca ccagccagac gccagcgttt
     6781 cagttgccag acccaggcca gcacttccgc caccgcggcg tacagttgac ccgggatttg
     6841 ttgaccaatc tccgcatgtc gatacagcgc tcgcgccagc ggcggcgctt caagcgtcgg
     6901 gacgttattt tcagcgccaa tttcacgaat gcgcagcgcg accagccctg cacctttagc
     6961 gaccactttc ggtgcgctca ttttgttttc gtcatactgc aacgctaccg aatagtgggt
     7021 cgggttattg acaatgacat ccgctttcgg cacatcggcc atcatccgac gccgtgcagc
     7081 agctcgctgc atctgacgga tccgcccttt aacatgaggg tcaccttcgc tttgtttgaa
     7141 ctcatcacga atatcctgcc gtgacatacg cagctttttc aggtggctga atatttggaa
     7201 aaagacgtca aatcccacca ttggaatgac accaagcacc accagcagtg cgcatagccc
     7261 taccaaatcc atcgcattac ccatggcggt aatcggagac tcggccatca agcgcatcat
     7321 ctgcggccag tgatgccaga gaaaaaaccc cgtcacgctg ccaaccagga tggttttcaa
     7381 aattgcttta agcaactccg cgccagtctg agccgagaac atccgtttaa tgcccggtag
     7441 cgggttgagt ttggaaaact tcggctgcaa ggatttgccg ctaaatacca gccctcccag
     7501 catgaccgga gaaatgagcg ccaccagcac cacgccgcta atcagcggca gcagcgccag
     7561 catggcttct ctgatcagca gaataatctg cccgaggatc agattcgggt cattgataat
     7621 actgtgatca aaatgcagcc cagcggagag catgcccgac aatcgacggg ccagcgacac
     7681 accgccaaac cagataacac taacgcccac cagcaaaatc agcagtgagg tcagttcacg
     7741 ggaacgcggg atttgcccct cttcccgcgc tttttctagt cggtgaggtg tgggggcttc
     7801 tgttttgtcg tcgctctcgt cagacacgtc gccaatcctg gaaagagtat taagcgtgaa
     7861 tgatgccaga gcgcaaagcg ttcaatggtt tgagtaaggg gcaaaacagg cgggatttag
     7921 ggcttttgct gccacacatc aagcatagtg tgcgtttgtc ggatgcggcg tcatcgcctt
     7981 atcagaccgc ctgatatgac gtggtcacgc cacatcaggc aatacaaatc aaaatccaag
     8041 actatccaac aaatcgtcca cctgatcctg actggctacc acaccggctt tgctggtatc
     8101 gacctgaggt ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg
     8161 gatgttttcc aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat
     8221 ccgcttaatg acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag
     8281 ttgcgcgtta gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt
     8341 taccagttca cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg
     8401 ggttaacgct tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact
     8461 gttcagcgcc cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg
     8521 cgcatcgggg atggcttccg ccgcttcggc aatggcctga tccagcccca gttcccgcaa
     8581 actgtcgcgc agcatacgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg
     8641 ctcgtcagca ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct
     8701 caaagatttt gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc
     8761 cactggcccc cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca
     8821 ttaacactgg caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc
     8881 catccatatt gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact
     8941 tattgagagc gtcgacgcca tcttccgctt cctcaacatt attgaatccc agctctttca
     9001 gcaggttacg cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt
     9061 ctttatccgc catttcacac tcctgattta aatacgtatc gcctgtccgg cactaatttt
     9121 tgccaacatt tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt
     9181 gatggcctcg cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca
     9241 tgcccccgcc tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag
     9301 gatcacccca accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac
     9361 cgaaggccga tgacggttaa ccgccgggcc atcgtgaatt ttgatttggt aatttgcgcc
     9421 actacgcgac agctccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac
     9481 acgttctccg tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc
     9541 aaaagagcgg gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag
     9601 cggcaacggt tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc
     9661 aatcagtttt tcagaactca acaacggacc cgccttcagc gttgtcggtg ctgacaatgg
     9721 cttatgtgct gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct
     9781 atacgccagc attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc
     9841 cagctccagc gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac
     9901 aacgggcatt ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg
     9961 catttcaaca tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcgac
    10021 cagcggatca ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt
    10081 catgatctgg cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt
    10141 taatccttac ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcaaggtgg
    10201 ctaaagtttt cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag
    10261 cagcgcaaaa tctcctgctg ggtattttga tcgaagtaga tcatgacgtt acgacagaag
    10321 atcgcatcaa acggccccgg cacggtgtac tgtttcgcca gtagattcag cggggcaaaa
    10381 tcaacatagt tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct
    10441 cgcatgaaat accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgataa
    10501 ataccgcttc tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag
    10561 cgtccgggcg cggtgcccaa tgtgtcagcc agcgtcatcg caatgctgta cggctcttcg
    10621 ccggtcgaag ccgccgcgct ccatacgcga tactcgccag aacggcgacg tgcgtgatcc
    10681 gcgagcagag ggaaatggtg tgcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa
    10741 ttgataaacg cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga
    10801 ccgaaatccg tcagtcccag cgaacgcaaa cgacgaacca gtcggttgta aaccatgtcg
    10861 cgtttatggt cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga
    10921 aaatgcgcgt cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca
    10981 cagggcagag atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt
    11041 tgtaactgcg ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca
    11101 cgcgacgaaa gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct
    11161 accagcgagg cgttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc
    11221 tcaatccccc gctgttgttc ttccgacgca gaggcaattt ctcccataat gtcgtccacg
    11281 cgggtcaccg aactgacaat atcgatcatg gtcgcggcgg cgttattcac cagtttcgaa
    11341 ccctgctgga cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc
    11401 gcgctacggc tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc
    11461 tctccggcgc gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca
    11521 attccgtcga taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc
    11581 atggtgtgag tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt
    11641 tttgccagtt ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat
    11701 tgctccatac tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag
    11761 agatcgttat tgcctgcgac aatctccgcg ataccaatgt gcatctcctg gcttccctta
    11821 cgcacatcac tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg
    11881 gcggtgatct cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg
    11941 ctgtcaaaat ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac
    12001 agcgcactgc tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa
    12061 atctgatagt tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc
    12121 tgccaggcct caaagttaac gtcaaacgca ttctgtgatt cctgcaccgg cgcagtgagg
    12181 aaatccgaaa gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgttgtgc
    12241 cagcgggcaa aacttttctc cgtctctttt tgcaacgccc tgacgtgctc gttgcccgca
    12301 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
    12361 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
    12421 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
    12481 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
    12541 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
    12601 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
    12661 aatactcgtc aaaatgtttc ccagtttgga gcttgttcag caattcgcag tcgtggctgt
    12721 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
    12781 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
    12841 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
    12901 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
    12961 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
    13021 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg agacggagtc ttcaatgagg
    13081 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
    13141 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
    13201 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
    13261 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
    13321 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
    13381 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
    13441 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
    13501 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
    13561 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
    13621 ttcgccaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
    13681 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
    13741 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
    13801 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
    13861 attccctggg ttggctgagc gaaataagct ccagtattgc cataatcaag ataatcaatc
    13921 agttcagtta acgctgtgtg atagttttta tatttttcat caatattacg actggtagcg
    13981 accatttcag gtaacggtgc catgcttttg aattttttat aatgagtcgc ggcctgggcc
    14041 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
    14101 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
    14161 caggttgacg tcagctcgcc ctgctgttcc cgtaattgat tggaaaccac aaagctcttc
    14221 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
    14281 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
    14341 cctgaaaaca agttaatctc gttatcggca agaagggggg aaactttatt gctgatgcca
    14401 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
    14461 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
    14521 ctgttcagca gtttttcgat gttcaccaga atcaacatcc ggtcgcccag tgcgcccagt
    14581 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
    14641 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
    14701 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
    14761 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
    14821 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
    14881 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
    14941 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
    15001 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
    15061 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
    15121 gatagttact ttcaaggttt ttaaccacaa cctggtgctg accaattaat tgatccacca
    15181 gcaaggcgta gcggcgaccg ccactttgta agatcaccac aattccctgg gtggcttcgg
    15241 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
    15301 gcacttccag cacccgctcg ccgccggcca gtggatgcag atcggcttca cggggttgca
    15361 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccgcaacg cgtacggaca
    15421 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtaccct
    15481 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
    15541 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc cgtggagaag cctggtgcaa
    15601 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
    15661 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
    15721 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
    15781 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
    15841 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
    15901 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
    15961 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
    16021 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
    16081 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
    16141 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
    16201 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
    16261 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
    16321 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
    16381 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
    16441 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
    16501 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
    16561 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
    16621 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
    16681 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
    16741 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
    16801 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
    16861 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
    16921 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
    16981 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
    17041 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
    17101 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
    17161 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
    17221 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
    17281 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
    17341 ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca tatagggttc gacatcggca
    17401 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
    17461 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    17521 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcagc
    17581 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    17641 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    17701 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    17761 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    17821 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    17881 gcttgattct tcatgcttcc tcggttgtca tctgttgttg cggatttttc accgcacgca
    17941 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    18001 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    18061 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    18121 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    18181 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgacc cccattacag
    18241 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    18301 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    18361 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    18421 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    18481 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    18541 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    18601 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    18661 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    18721 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    18781 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    18841 tggggcaaaa aaaagcagcg gtacgtcgtt accgctgctg gaatgttgcg cctcaccgta
    18901 tcagttaaac agcctgtact ctctgttcat ccagcagttg tgggataata tcggcaggat
    18961 tctgggaaag tttacgtctt tttactgccc gggatggcgg ttgacataag ctgcaggcaa
    19021 agctgccaac aggctggtga gcgtgggtaa taaaattgcc gccgcagcag ttgcagctgg
    19081 aaagttgcag taatccactt tcaacaaacc gcaccaatgt ccaggcacgg gttaatgcca
    19141 gcagtggtcc ttcttctgct tgtgggcact gttcaaggta taaacggtag gctttgatca
    19201 ccgcatcgac gccattacac aaaccggttt tcagtaaaaa ctgccatgca ttacagaaca
    19261 tcgaagcatg aacgttttgt tcccaggtca taaaccagtc ggttgagaat ggcagcatgc
    19321 ctttcggcgg tgggcttccg cgcagttctt tataaagttt tatcaggcgt ccgcgactta
    19381 actgtgtttc gctttccagc atctgcaaac gagcgcccag ggtgatcaat tccattgcca
    19441 gctgaatatc ccgcgcttcc tgaacaatgc ttttttcact catgatcagg cccttttctt
    19501 gcgcagcgct tcttcaggct gattaacatc attcagcaag cgtgttgaga gcatgatgcc
    19561 ggtatgaatt tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag taatcgtctg
    19621 gtggctgtca aaacggaagt gacaaaccag ttgattggtt tctgccagct taaccatttg
    19681 cggaagagtc agtgccgcta acgttgtcgc catttcttca tttatgccga gacgaaacat
    19741 agcggacgct ttgtcctgaa caatcaaacg ctgtgcaagt agtaaatatg acaagttgat
    19801 gtcataaatg tgtttcagca actcggaggt atgcattatt cccacccaga ataaccaact
    19861 ttatttttat gcggtttcac cgcaccccgt gatgtcgccg ggaagccccg gtaaaaaata
    19921 attagcatta gaatagttgc gataagctgc aataagcaga accacctttt tggtttaata
    19981 tgtccttaca aatagaaatg ggtctttaca cttatctaag atttttccta aatcgacgca
    20041 actgtactcg tcactacacg cacatacaac ggaggggggc tgcgattttc aataatgtgt
    20101 gatgcagatc acacaaaaca ctcaattact taacataaat gtataagtca tacttttgtt
    20161 ttgggtgtat ttcaatctgt taaaaagttt ttcgctacgc tagcaagcaa aaatgaaaca
    20221 ggaataatcg aaatgggatg ttgcgcacag ttaaaataac tcaccgtaaa taatcatctg
    20281 ctataaataa tcactttcat gcaataccag ataagctatt tttaaacaga cacttaccgc
    20341 acaacaaact aattaaatac aaagaaaggc aacaatcaac ttaatgttaa tgaaatgttt
    20401 tacatatatt aaccacttgt tta
//
LOCUS       DAZGOB010000084        20354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGOB010000084 DAZGOB010000000
VERSION     DAZGOB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.084"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..483
                     /locus_tag="ACHLIF_003262"
     CDS             <1..483
                     /locus_tag="ACHLIF_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFO9607228.1"
                     /translation="GGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGALWAGAMAAPV
                     LLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNYLGGWLTEAW
                     QKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNVWNGGNYGLW
                     "
     gene            470..1819
                     /locus_tag="ACHLIF_003263"
     CDS             470..1819
                     /locus_tag="ACHLIF_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFO9607229.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLTEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            1803..2927
                     /locus_tag="ACHLIF_003264"
     CDS             1803..2927
                     /locus_tag="ACHLIF_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO9607230.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            2917..3531
                     /locus_tag="ACHLIF_003265"
     CDS             2917..3531
                     /locus_tag="ACHLIF_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO9607231.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            3524..3961
                     /locus_tag="ACHLIF_003266"
     CDS             3524..3961
                     /locus_tag="ACHLIF_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO9607232.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPVGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHARLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            3961..5043
                     /locus_tag="ACHLIF_003267"
     CDS             3961..5043
                     /locus_tag="ACHLIF_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO9607233.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            5034..5594
                     /locus_tag="ACHLIF_003268"
     CDS             5034..5594
                     /locus_tag="ACHLIF_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO9607234.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHKEENI"
     gene            5594..6505
                     /locus_tag="ACHLIF_003269"
     CDS             5594..6505
                     /locus_tag="ACHLIF_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9607235.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENAETARNNLDVYSRSEGDNRYLRR
                     DQNGADIQDKGTFIDNLGLRETVNKAADALPSAGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNTWRTVSSV"
     gene            complement(6540..7061)
                     /locus_tag="ACHLIF_003270"
     CDS             complement(6540..7061)
                     /locus_tag="ACHLIF_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9607236.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            7418..7603
                     /locus_tag="ACHLIF_003271"
     CDS             7418..7603
                     /locus_tag="ACHLIF_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607237.1"
                     /translation="MFISCVPSKATPLILRAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISTLKGFSTSF"
     gene            7617..8177
                     /locus_tag="ACHLIF_003272"
     CDS             7617..8177
                     /locus_tag="ACHLIF_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9607238.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            8277..10316
                     /locus_tag="ACHLIF_003273"
     CDS             8277..10316
                     /locus_tag="ACHLIF_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9607239.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENGVNVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSAWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGAELLKNGGLISCRFKVPGDLVANQYVMALYWPVSSLPQGVTLTGDA
                     GNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     IPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            10463..10645
                     /locus_tag="ACHLIF_003274"
     CDS             10463..10645
                     /locus_tag="ACHLIF_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9607240.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            10681..10926
                     /locus_tag="ACHLIF_003275"
     CDS             10681..10926
                     /locus_tag="ACHLIF_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9607241.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENDPQPVEQPEPVQPGTDGTMM"
     gene            complement(10965..11431)
                     /locus_tag="ACHLIF_003276"
                     /pseudo
     CDS             complement(10965..11431)
                     /locus_tag="ACHLIF_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11546..11746
                     /locus_tag="ACHLIF_003277"
     CDS             11546..11746
                     /locus_tag="ACHLIF_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9607242.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPNHQHA"
     gene            11700..12437
                     /locus_tag="ACHLIF_003278"
     CDS             11700..12437
                     /locus_tag="ACHLIF_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO9607243.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESIFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRANVHKFRQFRYIRFLNKRAK
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            12567..12848
                     /locus_tag="ACHLIF_003279"
                     /pseudo
     CDS             12567..12848
                     /locus_tag="ACHLIF_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(12919..14343)
                     /gene="lpdA"
                     /locus_tag="ACHLIF_003280"
     CDS             complement(12919..14343)
                     /gene="lpdA"
                     /locus_tag="ACHLIF_003280"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO9607244.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(14668..16560)
                     /gene="aceF"
                     /locus_tag="ACHLIF_003281"
     CDS             complement(14668..16560)
                     /gene="aceF"
                     /locus_tag="ACHLIF_003281"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO9607245.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(16575..19238)
                     /gene="aceE"
                     /locus_tag="ACHLIF_003282"
     CDS             complement(16575..19238)
                     /gene="aceE"
                     /locus_tag="ACHLIF_003282"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO9607246.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(19399..20163)
                     /gene="pdhR"
                     /locus_tag="ACHLIF_003283"
     CDS             complement(19399..20163)
                     /gene="pdhR"
                     /locus_tag="ACHLIF_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO9607247.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         4798 a         5199 c         5434 g         4923 t
ORIGIN      
        1 ggtgggttac tttcgtctgt cggtagccgc atggggctgg ggcgtttctc cggctttttc
       61 cgcagtgccg gtggactggc cggccggctg ggtggaggtg ccctgtgggc tggcgcgatg
      121 gcagccccgg ttctgctgga tggcagcgcc agtgccacag acaagggaga agcggtcggc
      181 tctcttgcag gcagtattgc cggtggtgcg ctgggggcgg cagccggacc tgtggggatt
      241 gccattggtt ctacggtggg cagctatctc ggtaactatc tggggggatg gctgactgaa
      301 gcctggcaga aattacgggg cagtgatgat gaaagcagtg gacaggcggt acaaaaagcc
      361 tctgcccgtg tggagctggt tgccccggaa gggtggcagg ctcgcagtat tgatattgat
      421 gacacgtccg gtcacggact ggatgtgaat gtctggaacg gagggaatta tggcctctgg
      481 tgacggtcgt ggtgcgttcc gtggtgtgcc gttcctcgtc tggcgtgagc agcgcgaacg
      541 tggtgggcga aacattgtcc gtcgggaata tccgttacgg gaaaccggtg gggcagacga
      601 tctgggacct aaactgactg aatttacgtt cagtgtactt gtgatggggg atgatgttca
      661 gacacagcga aatcgtctgc gtgatgccct tcgtgctccc ggtgccgggg agctgcttca
      721 cccggattac ggcacgttaa atgtgctgat aaacagcttt gaaagtcgtt ataacgccgc
      781 tgaacagggc gtggttgagt tcaccattaa tgtcacgccg gtaagtgatg ataccgcacc
      841 tgctgtcaca caggacacgg ccgccattct ggagcagaag agtaccaccg cgctcgggca
      901 ggtttttgaa accctggaag cgggctggac ggtgatctct gacggaatgc acgatgtcca
      961 ggcaatgacc gaaaccatca gtgataaggt gtccgccctg gaaaatgccg tttccggcat
     1021 ggggattgta caggatatca gtgcctttac tgccacgttc acggcgctga aagggaatgc
     1081 cacggcatta ctgacagcac cttcccgcat ggcctcatct tttgcggggc ttttcagtgc
     1141 cctgatcacc ctgccatcgc tgccgtcact gtcttcagga ggcctgaata cccggcccgg
     1201 tggcagtatc ggccggacga catccgccgt ctctcaggga atgccgcaac tgtacaggac
     1261 attatcttcc ctgcgttacg tgctggatga gcaggataat ccgcagactc tcatcggtct
     1321 gacaccggca gcacaaaaga atattcgtct gatacgggcg gtaatgcaga gtgctgccgt
     1381 ggtggcccag gcacagaccg tgggaaagct gctggatcag gttctcagtc aggaaaccct
     1441 gccggacagt gacgcagccc accgtacctg gcctgtctgg ctggaaagtt cagttgatct
     1501 tcagcgcatt aaccgtgact taagcgaagc gctcgaacgg caggtgatga cactgtccgg
     1561 gcaggggtat accgccacgg cgctgacgct tcgtgatgcc agactggcac tgacagagga
     1621 tctgaataca cggggagttc agctacccgg tgcaacagta gtgactgtac gtaccaccga
     1681 gcctgcactg gtgaccctgt accgtgccac cgggaacagt accggctggc aacgttttgt
     1741 gcgccgtaac ggtattgttg atccgctgtt cattcccgga ggccattcag tggaggtgat
     1801 tagtgagcag cagggttgaa ctgtatctgg gcggtgagat tttttccggc tggctgacgg
     1861 tgagtgttcg tcgctctctt gaacatctgg cgggctcctt tgaactgggg gtaatgatgc
     1921 ccggtgtacg ccttccgtca tccgtccgtg ccggtcagtc tctggaattg cgcattgacg
     1981 gtcagcctgt gatcactggc tggctggatc aggtccggca gcgcatcagc gccacgcgtt
     2041 ttcagatcac gctcagcgga cgggataaaa ccggtgacct ggtggactgt tcagccattc
     2101 atccgggcag ccagtggagg aaccgcacgc tggagcacat tgcttcagat ttgtgtgctc
     2161 cgttcggggt cacggtgcgc tggcaggtaa atgatgcaac ggcagcccgg cccttttcca
     2221 ccttcacact ggaaaactca gaaaccgtgg cagatgcgct gacacgggcc gcgagacaca
     2281 gaggcgtcct ggtaaccagt aatgccgccg gtgaactggt tttcactcag gccggcagtc
     2341 agcgtggcga cacgctgacg ctgggcgaaa atctgctgga tttggatcac aacgtggatc
     2401 accgtctgcg ccacagtgaa taccgtgtac gggggcacgg gcgtggtggt ggtcatgccg
     2461 gggatgcact gacagccgga acgctggccg cacccgttgg tacggtgaca gacagtgcca
     2521 tccaccgtta cagaccgaaa attgtgctgg cggatcatgc tgttgatgca gacggtgcac
     2581 gccagagggc tgtccgggaa atgcgccggg cggttgcccg ctctgtgcgc ctgacagcca
     2641 ccgtgcggca ctggtttcgg gagaacggcc agttgtggga tatcaacctg ctgacggctg
     2701 tcacggctcc ccgcaccgga gtggaagagc gtgatcttct tgtctgtcag gtggagtttt
     2761 cgctggatgc aaatcacggc gaaaccaccc gtctgattct ggcaccccgt gacggcttta
     2821 ttgttccggc agagccggga aacagcggaa gcggaaatgc gggtgacgtg gacgccttcg
     2881 tgcgggcaca gatgaaaaaa caggggatta aattcaatga tgaatgacga agtcatcagc
     2941 cgccttctgg cccccgtaat gcggggtgtt cgtctgctgt tcgggcgtgg tgtactgacc
     3001 ggcacaacgg acacgctgaa aatccagaat gtgcaaatca ccggtatgga tggtgaaacc
     3061 tttgatgacg ttgaacgccc ccagcagtac gggcaaatca gcgtccccct gcctggtgcg
     3121 gaagtttttc tggcctgtgc tggcggacaa cgggatcagg ccgtggtgct tgtggtggaa
     3181 gatcgccgca gtcgcccgac cggacttacc gccggagata ccggcgtgta tcaccatgag
     3241 gggcaccgta tacgtctgac aaagaacggc cggatcattg tgacctgtaa gacgctggag
     3301 atttacgccg atgaaggtgt tcaggtggat acaccggagg ctcactttac cggtaatgtc
     3361 acagtggata agaacctgca tgtcaaaggc aatgtgtcca ttgacggcac cggaagatca
     3421 caggggacgt tcacgatgtc cgaagcggtt attgccggga tcacctattc aggtcatgtg
     3481 catcacgata acggtgaagg cagcaaaacg ggaggaccag agaatggctg atattgctgt
     3541 cgtctgggat cagggttgcg gttccctgca actgaacggc gcagatcttc tgacggataa
     3601 cagcctgctg actgcggtca ttatttcact gtttacggac aggcgggcgc tggattccga
     3661 tgaaatccct gacggcaccc gtgaccgtcg gggatggtgg ggagacagtt tccgggagcg
     3721 ccccgttggc tcccgtctct ggctgttaag ccgtgaaaag acgctgtcct ccgtggtcag
     3781 ccgtgcacag gcctatgctg atgaagcgct ggcgtggctg cataaaagcg gtgctgccac
     3841 atccgtggta tgtcatgcca tgcgtgtggg gcatgctcgc ctttcgcttt ccgtgaaaat
     3901 caccctgccg gacggaagca gacatccgat gattttttat gctgatatga agggggaatg
     3961 atgccttatc agcccttacc actggcgcaa ctgatcacac agacacaaca ggatatcagc
     4021 cagcgcctgc ccggttcgca gccgggcgtg aatgaaacca ccctgaatgc cattgcgtat
     4081 gcactggcgg gtctgtcagc acaggagcat gaacatctgg cctggatctc ccggcagata
     4141 attccgacag aagctgatga agccgaactc ctgaaacact gcgcattctg gggtgtcatc
     4201 cgtaaaccgg cttcccgcgc tgacggaccg gtacaactga tgctgaccac ggatgcaggg
     4261 atcacggaag gcgtactcct tcagcgaagc gatggtgttg tgtaccgcat caccggctct
     4321 gcgaccggaa aagccggaac actgaatgtt aatgtggagg cggaaagtgc ggggcgcgcc
     4381 ggaaataccc cgaccggaac ccgcctgtcc tttatcacgc cacaggcggg catcaaccag
     4441 acagccacgg tcaccggcac gggactcacc ggtggtgcgg atgtggaaac ggtgccggag
     4501 ttgctgtcca ggctggtatt ccgggtacag aacccgccat cagggggaac acagtatgat
     4561 tttgaacgct gggcacggga agtaccgggc gtgacgcggg catggtgtaa gcctgaatgg
     4621 cctgaggcgg gtagtgttgg tgtgactttt gttcaggata ataaccctga cattttcccc
     4681 ggagaaggtg atgtgaagcg ggtggcggat tatatccgca gtcatgatga tccggcgacg
     4741 ggccagcccg ttggtcagcc acttgggccg acaatcagcg tgtttaagct gaccaataag
     4801 ccggtggcgt ttgagatcag gattgtaccc aaaacgccgg aaaatcaggc tgccgtaaaa
     4861 caggcattaa cggacctgct ttataacgaa tcgcggccgg gtggacttgt attgccttca
     4921 tcattctggc gggctgttgc aggggtgaaa ggactggagg attttgaagt tcgcagtccg
     4981 ctgaagtccg tgatggccgg agatacagag ttgctgaccg tgggggaaat cacatggctg
     5041 taaccctgac cccgcatcag cgcgccctgt tgcagttgct gcctgacggg ctggcatggg
     5101 ataagcggcc gtcatccgtt cttgcggctt tgtgcctggg cctcagtcat tccacggagc
     5161 gtgtttcctg gaccggtaac cagatgctgg cagaacgttt tcctgattca tcccgtctgc
     5221 tgctggaaga ctgggagcgt tatctggggt taccggaatg tgatatgacc ggcgcaacca
     5281 ttcaggaacg tcagcgttat gcagggaata aataccggat gaaaccctct cttaaccgtg
     5341 aattttatat ccggtttgcg gcagagtttg gttatgaaat agatattcag ccatcaccgg
     5401 attcacagtg ggtcagtatt gtcacgataa acagtgaaac cggctaccgg aatatgaatg
     5461 tgctggatga tattctcacc ccgctgcgta tttatgaggg tggtgcgctg gaatgtattc
     5521 tgaaccgtta taagcctgca tggcagacgt ttatttacgt gtatgcaaac agtcacaaag
     5581 aggagaatat ttaatgttcc atgttgataa taattccggc gtggcgaata tgcctgaact
     5641 ggctccggcg cagagtaata ccaccacctg gtttaccgaa ggtgacggac aaaaaggtat
     5701 cagctggatt ggtcaggact ggctgaatat tctccaggcc gaactgctga atattctggc
     5761 tgaagccagt attcagccgg ataaggcgca gttaaaccag cttacgctgt ccattaaagc
     5821 cattatcgct gcgaatgcct tttcccggaa aaacaacctg aaagaaattg ctgatgccgg
     5881 tgcggaagcc cagcgtctgg cccgtggtta tcttggtctg gggacgcttg ccacaaaaaa
     5941 cagtcttggt cccgctgacg ttaatgccct ggcgaaggat cagaaccttg ccgacctgga
     6001 gaatgcggaa accgcccgta ataatctgga tgtttacagc aggagcgaag gtgataaccg
     6061 ttacctgcgc agggaccaga atggcgcgga tatccaggac aaaggaacct ttatcgataa
     6121 cctcggttta cgggaaacgg taaataaggc tgctgatgcc ctgccatcgg ctggaaccgc
     6181 cgtggcagca aacagactcg ccaccccaag aaatattaat ggtgttcctt ttgacggaac
     6241 gctggatatc aacatcacgt caggaatgac gcagtcaacc gcagatggcc ggtatgtaca
     6301 gaatgttcag cttggagcac agagctatca ttcacccgga ggcaatgaaa tgtcatggaa
     6361 ttacagcgca ccttccggtt gtatgctttc cggtattaac gtgcaggaaa ccggctcccg
     6421 gtctgcggac aatatcggtg gggtctatta tcgcccggtt cagatttata ttaataatac
     6481 ctggagaacg gtaagttcag tgtaaaccac agaaaagggt gctgaatgca ccctctgaat
     6541 tattccggtt tttccggcca gggaatcgcc tggtaatgcc cctcactggc aatatcatta
     6601 aagtgcattt ctttaagctg tcggatatac gccatccagc gcgtcagctg taatttattt
     6661 tcatcgctaa tcatgtccag ttttaattct gttttccagt catccacggt ttcgttggct
     6721 tcctggagca gcgatgagcg acgcttttca gcaaccgtct tccagtttac cggcaccttt
     6781 gatatcaggc catcagaaaa ctgccagtta ccgtcaatat tcacaccttc cggcagttcg
     6841 tccacttcaa caacagtaaa acctgccgga tataaagcag agacatcttc agcaacagag
     6901 cagataatac ccgttacagg cgaaatacag agtttgtatt tctttgtaaa aagcgggata
     6961 gattcataaa aatcccttcc gtcctcactc tggaaatact gaacatcctc accatagggt
     7021 ttgtctttcg ggtaatatcg tttgacatta atcagttgca taaacatcac catgtaaatc
     7081 aatgataaaa acaggaataa tactatcgcc atatacaggc aatgcttgtc tgaaataacg
     7141 catatttcag atgctggctg ctgtgtgcta tgcccccgtt aattaaatac ttgcagcgga
     7201 tacccaatgc ccattaatac aatattgtat ctgtctgtaa taaacacggt catcaccacc
     7261 acgcatttcc cagtctccgt cagtcttaaa accggtaatc acatttcctg atgacaggga
     7321 gtaattatat tccagtccgc cgcttaatgc ctgcgctccc agtcttaccc ctgtaacata
     7381 acgctgattg gccgttgatt ccggcattcc tgacgtaatg tttatatcct gcgtgccgtc
     7441 aaaggcgaca ccattgattc tgcgggcatt tgccagcctg cttgctgcaa cagccgtacc
     7501 gtttgatggc agggcattcg cggccctgtt taccgtatcc cgtaaaccga ggtttcaggg
     7561 atgccgtata tctactttaa aaggcttttc cacctctttt taaggaaggt aaatacatgc
     7621 tgattggcta tatacgcgta tcaacaaatg accagaacac cggtttacaa agaaatgcgc
     7681 tggaatgtgc aggatgtgag cagatttttg aagacaaaat gagtggtaaa acgacacagc
     7741 ggccgggact gaagcgggca ctgaagaaac taaaacccgg tgacacgctg atggtctgga
     7801 aactggacag gctggggcgc agtatgcgcc accttgttgc gctgacagat gaattacagc
     7861 aacaggggat aaattttcgt agtcttactg acaacattga tacgtcaacc cccatggggc
     7921 gcttcttttt tcacataatg ggtgcgctgg cagaaatgga gcgtgaatta attgtcgaac
     7981 ggacacgggc tggtcttgca gctgcccgtg aaaggggacg aaccggcggc agaccccgcc
     8041 gtctcacgca ggaacaatac gagcaggtcg ggcgtttact ggccgggggt gtcagtcgaa
     8101 gacaggtggc acttattttt gatatcggtc tttcaaccct ttatcgttat tatcctgcga
     8161 gcgtttcgga aaaataagac gctctctttg ttgggccttt actgaattaa cgcaaaatgg
     8221 cgtttatcgg tctgtggata tgataattca gtttattcag gaacaggctg attattatga
     8281 cgtccaaatg ggttcaacta tcatcaatgc ccggaaactt tactgttaaa gtttccggtg
     8341 gtacagcggc atttcttgag gctccctttc ctccggccga aacaaaagga ggaatgacat
     8401 ttgctgactg tctcatcagt tttaatacac gggattgcct gtgggtaagg ccagtatccg
     8461 gtgatccgag tgtggaaatt accggagcgg gtattggtgc ggtcattccg ttaagtgctg
     8521 atgttgccgg tactgctgaa ccgtcagact gggataatgc tgaaacacat acccgtccgt
     8581 caggaaatga aactgcttcc agctcccctt cctggtatta cgtggtggtt cttgctggtc
     8641 agtcaaacgg tatggcttac ggtgaaggtc tgccactgcc ggagacatat gaccgccccg
     8701 aaccgcgtat tatgcagtta gcccgtcgca gtacagtcac gccgggaggt aaagcctgtc
     8761 agtataacga catcattctg gccgaccact gtctgcatga tgtgcaggat atgagtggaa
     8821 aaaatcaccc gaaagcggat gtggctaaag ggcaatatgg caccgtgggt caggggcttc
     8881 acattgccaa aaaactgctg ccgtttattc cggcagatgc ggggattctg ctggttccct
     8941 gctgccgtgg tggttctgca ttcacggcgg gtgctgacgg cacgtacagt gacagcacgg
     9001 gtgcttcaga ggactcagcc cgctggggtg tggataagcc gctgtataag gacttaatca
     9061 gccggacaaa agcggcactg gcgaagaacc cgaaaaaccg cctgcttgct gtggtgtgga
     9121 tgcagggcga gtttgatatt gatgcgaagc cgacggagca ttctgcgctg tttctggcga
     9181 tggtggaaaa attccgcgca gacctggctg aacaggcgga acagtgtacc ggtggcagtg
     9241 ctgctggcgt tccgtggatt tgtggcgata ccacgtattt ctggaaacag aaaaatgaac
     9301 cagcatacca ggcaatctat ggcggctaca aaaacaaaac agataaaaac attcatttcg
     9361 ttccattaat gacagatgaa aatggcgtga atgtacccac caacaatccg gcagaagacc
     9421 cggatattga atccattggt tattacggct caacgtggcg taacagtgcc gctacctgga
     9481 catctgcgga ccgggccagc catttcagtg cctgggcgcg ccggggtatt atttctgacc
     9541 gtctggccac ggctgttctg actcatgcag ggcgaaccac agtaaaagcc gatgttccat
     9601 cttctgaaac tgaagcacca gtgccgtcac cttcagaaac tgaagcagta accacaacac
     9661 tgctgtctta ccgtgtcagt gagtcagaag gaaatctgaa agcccagggc tgggagccgg
     9721 ctggcggtaa agctgaaatt atcagtgatg caggaggcac aggtggtaag gcaatgaaac
     9781 tgaccaagga aacaggtaaa tcatcctggt atctggatca tgatgccggt actggtgctg
     9841 aactgctgaa aaatggtggt ttaatcagtt gtcgttttaa agtacctggc gacctggtgg
     9901 caaaccagta tgtcatggcg ctttactggc cggtttcctc tctgccgcag ggtgtcaccc
     9961 tgacagggga tgcagggaat aatctgctgg catcgttcta catccagaca gacgcaaaag
    10021 acctgaatgt gatgtaccac aatgcaaaag tggcaacaaa taaccagaaa ctgggaacct
    10081 ttggtgcatt tgataatgaa tggcatacgc tggccttccg ttttgcagga aataacagcc
    10141 ttcaggttat tccggttatt gatggtcagg atggtgccgc gtttaccctg acgcagtcac
    10201 cagtaggaac tttcccggtg gacaaactcc gcgtaacgga tatcaccaaa aatgcaactt
    10261 acccggtgct gattgacagc attgtggtgg aagtgaaaaa agcggtaaca gaataaaaaa
    10321 atcccgccgg tcatgatatg gctggcggga gctacctaac ccataaagga aaataaaaat
    10381 actcccgaag gagtggttat cagtcgcttc gatgttttta cccgataacg aattatgtag
    10441 tcaatatcaa aggtaaggtt atatgacatt cgttcataca atgctgcttt atttctgcgc
    10501 tgtggtcagt gcgttatatc tggtgagcgg cggttataag gtcatcagaa attatattcg
    10561 ccgtaaaatt gatgatgcgg cagctgaaaa actcagtaaa acagcacagg caccgtcatc
    10621 cccaaacgac ccgaccccgc tctgataacg gggcaatata cctgcaaaag gagaatattc
    10681 atgccagaga ttaaaggcac ggttactgaa gagcttgtca aacaggcact ttattctgaa
    10741 gaagtgaacc gcgtgctgaa ggcgcaggtt cgcaaggatt ttgaggcaca aatcgacgca
    10801 tatgttgatg aagtgctggc ccgaatggtt ggccgttctc cggctgaaaa cagcacggaa
    10861 aatgatcctc aacccgtaga gcaaccagag ccggttcagc ccggaactga cggtactatg
    10921 atgtaacaat accgaccggc aggtttccct gccgggtgag tttgttaatg cacgttttgt
    10981 gtattttccc tcctgggaga acaggcagga catatcagca tcctgaaaca ggaagcattt
    11041 ttgggtttgt atgtcttgcc attttgagat cgagaacggc gatagatgag ctgtaaggga
    11101 gtcttacagg cagggcaaac aggctctttc atccatgata aagatctcct ttttatgcat
    11161 ctctgcttta caggagactg gggaatatca taggatacgc cacgagctcg aagtaaagca
    11221 ttgatgcaaa attgtctaaa tcggcccaga atagttcgcg ctatctgggt cgctctttat
    11281 gtatgtagta tcccattgat ttgaaacata ctacatatac gcgcgatcct accttcacta
    11341 tataaaaagt caataaaatg atcattttat gatcgttttc accacctaac tcttcgtttt
    11401 ctttaagtaa ataatcacct acctgaatca ttgttgatga cttttcaatc tatttgttgt
    11461 atcaattaaa caaatcgaat cgatcgtttt tgtcgatcga ttgcatgaga aaggcatcaa
    11521 caacaaagca ggaggttatg cggccatgta tcgaaacata cggtgccgac actgcaataa
    11581 gctactggca cgggccagtt tcagttatct ggaagtaaaa tgtccgcgct gtaaaaccct
    11641 caatcaaatc acatctccga gcgccacaga gcaccccaca tacacaagga aatcctgtcg
    11701 tggggaacag aaagcaagtc acatcccgaa tcatcagcac gcctgaactt attcgctata
    11761 acgacaatat cgttggttat ggctcccgtg agttgcgggt tgagacaata agttgctggc
    11821 tggccagact ggtcatcgtc aataaacatt acagccaccg tttcgtaaat aactcctacc
    11881 ttcatctggg catattttca gaacgggaac ttgttggcgt aatgcaatgg ggttacgccc
    11941 ttaacccaaa cagtggtgca cgcgtcgtaa cgggtacgca gaaccgagaa tatatggagc
    12001 ttaaccggct atggctgcat gactgtatgc cgagaaactc cgaatcaagg gcaatcagtt
    12061 acgcgctcaa gctaatcagg cagctttatc cgcaggtaca gtgggttcag tcgtttgcag
    12121 atgagcgttg tggctgtctt ggtgtcgtgt atcaggcaag caattttgat tatgtaggca
    12181 gccatgaatc aatattctat gaacttgatg gcgagtggta tcacgagatt tgcaggaatg
    12241 ccattaaacg aggcggacaa cgaggtgaac atctgagggc taatattgac agagccaatg
    12301 ttcataagtt ccgacagttt cgttacatcc ggtttctgaa caagagggcc aaaaaacgcc
    12361 tcaacacaaa gctattcaaa gtccagcctt acccaaaacc acaaacagtt aaaactggtt
    12421 tgaaagagag tgaatgagcg cttaaaaact cattctaaaa ctcaaaagaa aagcgccgat
    12481 ttagtatcaa ataagttgcg tatcggcgtt tttcagcagt atcaaatgaa atgcaaatcg
    12541 gtatcaaaaa aatcgccgcg ctataagacg cagcgcgtga gcgtattcag tcagcctcgc
    12601 ctgagcagcg ccaggtgttt aaggaaaaag tacagcagcg cccactgaac caacagcaac
    12661 gtgataacgc ccgccagcgt gttcaatcag catcacctga acaacgtcag gtttttcggg
    12721 aaaaagttca ggagagccgc ccacaacgtc taaacgacag taaccatact gtcaggctga
    12781 ataacgagca acggtcagca gtacgcgaac gtctctctga gcgcggagca aggcgacagg
    12841 aaaggtaaat tgcagacgta aaaaaagcgg cgtggttagc cgctttttta attgccggat
    12901 gttccggcaa acgaacaatt acttcttctt cgctttcggg ttcggcaggt cggtaatgct
    12961 accttcgaac acttctgccg ccaggcccac agactcgtgc agagtcgggt gcgcgtggat
    13021 ggtcagtgcg atgtcttcag catcacaacc catttcgatt gccaggccga tttcacccag
    13081 cagctcgccg ccgttggtac cgacaatcgc accaccgatc acacggtgag attctttgtc
    13141 gaaaatcagc ttggtcatac cgtctgcgca gtcggaagcg atagcacgac cagaagcagc
    13201 ccacgggaag gtggcggttt catagctgat gcctttctct ttcgcttctt tctcagtcag
    13261 acctacccat gcaacttctg gttcggtata ggcgatggac gggataactt tcggatcgaa
    13321 gtagtgtttc ttaccggcga taacttcagc ggcaacgtga ccttcgtgaa cacctttgtg
    13381 tgccagcatc ggctgaccga cgatatcgcc gatagcaaag atgtgcggta cgttggtacg
    13441 cagctgtttg tcaacgcgga tgaaaccacg gtcgtccact tccacgccag ctttgcctgc
    13501 gtcgaggttt ttaccgttcg gcacacgacc aatcgctacc agcacggcgt cgtaacgctg
    13561 cggttcagcg ggtgcttttt tgccttccat cgtcacataa ataccgtctt ctttcgcttc
    13621 aacggcggta actttggttt ccagcatcag gttgaatttc ttgctgatac gcttggtgaa
    13681 gactttaacg atgtctttgt cagctgccgg gataacctgg tcgaacattt caaccacgtc
    13741 aatctgtgaa cccagcgcgt ggtatacggt gcccatttcc agaccgatga taccgccacc
    13801 cattaccagc aggcgttctg gtacttcttt cagttccagc gcgtcagtgg agtcccagat
    13861 acgcggatct tcatgcggaa taaacggcag ttggatcggg cgagaacccg ctgcaatgat
    13921 cgcattgtcg aagttgatca cggttttgcc gttctcacct tcaacttcca gggtgttagc
    13981 cccggtgaat ttacccagac cgttgaccac tttgactttg cggcctttcg ccataccagc
    14041 cagaccaccg gtcagctgat tgatcacttt ctctttccag gtacgaatct tgtcaatatc
    14101 ggttttcggt tcgccgaaga cgataccgtg ttcagccagc gctttggctt cttcgataac
    14161 ttttgctacg tgcagcagtg ctttagaagg gatacagccg acgttcaggc aaacaccgcc
    14221 aagggtgttg taacgttcta cgattacggt ttccagacct aaatcagcgc aacggaaggc
    14281 agcggagtaa cctgcggggc ctgccccaag taccacgacc tgagttttga tttcagtact
    14341 catcatgacc tctatatatt tatctccggc ggtcataccc gtcgtctttc aggccgcagc
    14401 ggcgttagct tcacgccctc accccagtca cttacttatg taagctcctg gggattctgg
    14461 cgcttgctac cttgctgcac ccagaaatcc atagggtatg tgaagtaata ccctaaccac
    14521 caccgggtcg ttctatccgt cggtatttta caaaattgtt aacaattttt aaacaacaaa
    14581 cggcaaccga tttgtctatt cgctaataac ctcaatacat tcatgagatt accagaaaaa
    14641 agccggccgt tgggccggct cttttactta catcaccaga cggcgaatgt cagacagcgt
    14701 gttgttaatg atggtaatga aacgggcacc atcagcaccg tcgatcacgc ggtggtcgaa
    14761 ggagagagaa atcggcagca tcagacgcgg cacgaactct ttaccattcc acaccggctc
    14821 catcgcggac ttggaaacgc cgaggatagc cacttccggc gcgttcacaa tcggcgcgaa
    14881 gtgggtagta cccaggccgc cgatgctgga gatggtgaag caaccgccct gcatttcgcc
    14941 cgcagtcagc ttaccgtcac gcgctttctt agaaatagtc atcagctcgc gagacagctc
    15001 gatgatgcct ttcttgttga cgtctttgaa taccggaaca accagaccgt tcggggtatc
    15061 caccgccaca ccgatgttga tgtatttctt cagggtcaga cgctgaccgt cttccgacaa
    15121 cgaactgttg aagcgaggca tctgctcaag agctgcagca acggctttca tgatgaagac
    15181 aaccggggtg atcttcacat ccagcttacg tttcgccgct tcttcgttct gctgtttacg
    15241 gaacgcttcc agctcggtga tatcggtttt gtcgaagtga gtaacatgcg ggatcatcac
    15301 ccagttacgg ctcaggttcg caccagaaat tttctggata cggcccagtt ccacttcttc
    15361 gatttcacca aacttgctga agtccacctt cggccacggc agcatgcctg ggataccgcc
    15421 gccagtcgcc gccggagctg cttctgcacg tttgatagct tctttcacgt aagcctgaac
    15481 gtcttcgcgc aggatacgac ctttacggcc agtgcccttc actttcgcca ggttaacgcc
    15541 aaactcgcgt gccagacggc ggatcagcgg agtcgcgtga acgtaagcgt cgttttctgc
    15601 aaattcagat ttgccttccg ctttcgcagc cggtgctgct gccggagctt cagcttttgc
    15661 tgccggagcc ggcgctgccg cttcctgttt cgcaggagcc gccgcaggcg ctgcgccttc
    15721 aacttcgaag atcataatca gcgagccagt tttcacttta tcgccaacgt tgactttcag
    15781 ttccttcacg acgcctgcaa acggagccgg aacttccata gaagctttgt cgccttctac
    15841 ggtaatcagt gactgttcag cggcaacttt gtcgcccact ttcaccatca cttcggtcac
    15901 ttcaacttcg tcaccgccga tatccggaac gttaacttct ttcacgccag ccgctggtgc
    15961 aggggccgct gccggagccg cttcctgttt agccgccgga gctgccgcgc ctgcttcacc
    16021 cgcgacttcg aagaccataa tcagcgagcc ggtagacact ttgtcaccca cgttcacttt
    16081 gatctctttc acggtgccag caaacggagc cggaacttcc atagacgctt tatcgccttc
    16141 tacggtgatc agcgactgtt cagcttcaac tttatcgcca actttcacca ggatttcggt
    16201 cacttcaact tcgtcgctgc cgatatccgg aacgttaacg tcttttgccg ctgcagccgc
    16261 tggtgctgct gccggagctg cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc
    16321 tgcaccgtcg gcggaatcga aaatcataat cagtgcgccg gtctgggttt tatcgccaac
    16381 agagactttg atctctttaa cgatacccgc ctgcggagac ggaacttcca tagaggcttt
    16441 gtcgccttct acggtgatca gcgactgttc ggcttcaact ttgtcgccca ctttgaccag
    16501 gatctcggtg atttcaactt catcagcccc gatgtccggt actttgattt cgatagccat
    16561 tattctttta cctcttacgc cagacgcggg ttaactttat ctgcatcgat gttgaatttg
    16621 gcgattgcgt cagcaaccac tttcttatcg atttcgccac gtttagccag ttcgcccagc
    16681 gccgctacaa ccacgtagga agcatcaact tcgaagtggt gacgcaggtt ctcacggctg
    16741 tcggaacgac cgaagccatc agtacccagt acgcggtagt cgtcagccgg tacgtaagta
    16801 cggacctgct cagcgaacag tttcatatag tcggtagatg ccactgccgg agcgtcgttc
    16861 atcacctgag cgatatacgg aacgcgcgga gtttccagcg ggtgcagcat gttccagcgt
    16921 tcacaatcct gaccatctcg cgccagttcg gtgaaggagg tcacgctata aacgtcagaa
    16981 cctacgccgt aatctttcgc caggatctct gccgcttcac ggacgtgacg caggatagaa
    17041 ccggagccca gcagctgaac tttacctttg ctaccttcaa tggtttcgag tttgtagata
    17101 cctttacgga taccttcctc agcaccttcc ggcattgccg gcatgtggta gttttcgttc
    17161 agcgtggtga tgtagtagta aacgttctct tgtttttcac cgtacatacg ctccagaccg
    17221 tcatgcataa tgacagcaac ttcgtaagcg taagccgggt cgtaagagat acagttcgga
    17281 atagtcagcg actgaatgtg gctgtgacca tcttcgtgct gcagaccttc gccgttcagg
    17341 gtggtacgac cggaagtacc gccgatcagg aagccacgcg cttgctggtc gccagccgcc
    17401 cagcacagat cgccaatacg ctggaagccg aacatcgagt aatagatgta gaacgggatc
    17461 atcggcagat tgttggtgct gtaagaggtc gccgctgcca gccaggaaca acctgcgccc
    17521 agctcgttga tcccttcctg cagaatctga cctttctcgt cttctttata gtaagcaacc
    17581 tgctcgcggt cctgcggggt gtactgctga ccgttcgggc tgtaaatacc aatctgacgg
    17641 aacagacctt ccataccgaa agtacgcgct tcgtcggcga tgatcggtac cagacgatct
    17701 ttgatcgact tgttcttcag cattacgttc agggcacgaa cgaaagcgat agtggtagag
    17761 atctctttgc tctgctcttc cagcagcgcg ccgaagtctt gcaggctcgg cagctcaagc
    17821 ttctcggtga agttcggctg acggcttggc agataaccgt gcagtttctg acgctgagcg
    17881 tgcagatagg tatgctcttc agaaccttcc gggaaggtga tgtacggcag tttttcgata
    17941 tctgcatcag acaccggcac attgaaacgg tcgcggatgt gacgcacgcc gtccatgttc
    18001 attttcttaa cctggtgcgc gatgttttta ccttcagccg cgtcgcccat gccgtaacct
    18061 ttaatggtat gagcaaggat tactgtcgct ttgcctttgg tttcctgcgc tttcttgaat
    18121 gcagcgtaga ttttcttcgg atcgtgacca ccacggttca gtgcccagat ctgctcgtca
    18181 gtccagtctg caaccagtgc tgcggtttca ggatatttac cgaagaagtg ttcacgaacg
    18241 tacgcaccat ctttcgattt gaaggtctgg tagtcgccgt caacggtttc gttcatcagc
    18301 tggatcagtt taccgctggt atctttacgc agcagttcat cccaacggct accccacatc
    18361 actttgatca cgttccagcc agcaccttcg aagatgcctt ccagttcgtt gatgatcttg
    18421 ccgttaccgg tgaccgggcc gtcaagacgc tgcaggttac agttgataac gaagactagg
    18481 ttatccagtt tttcacgggt agcgatggtg atcgcacctt tggattccgg ttcgtccatt
    18541 tcaccgtcgc cgaggaacgc gtaaacggtc tgtttagagg tatctttcag gccacggtgt
    18601 tccagatatt tcaggaattt agcctggtaa atagcaccaa tcggccccag acccatagat
    18661 acggtcggga actgccagaa ttccggcatc agtttcgggt gcggatagga agagaggcca
    18721 ttgccgtgaa cttcctgacg gaagttatcc agctgctcct gagtcagacg accttctagg
    18781 aaagcacgag cgtacacgcc cggggagatg tggccctgga agtaaaccag gtcgccgcca
    18841 tcctgctcgt tgcgtgcacg gaagaagtgg ttaaagcaca catcataaat ggttgcggaa
    18901 gactggaagg acgccatatg gccgcccagt tcgaggtctt ttttcgacgc acgcagcacc
    18961 gtcatgatgg cgttccagcg gatagctgaa cgaatacggc gttccagttc cagattaccc
    19021 ggatactccg gttgttcttc aacggggatg gtgttgatgt agttgctgat acctgtgcct
    19081 gcggctacgt ttacaccgcc tttgcgggct tcagcaagca gttggtcgat cagatactga
    19141 gcacgctcaa caccttcttc acggatgacc gattcgatcg cctggagcca gtcgcgagtt
    19201 tcgatcggat ccacgtcatt tgggaaacgt tctgacatgg gttattcctt atctatctaa
    19261 taacgttgag ttttctggaa cctgtcccat tgaactctcg ccggaaagct caataagaca
    19321 ggttctacgt ttagttgccg cgcttttata tgcgcttgat ttacaacatc ttctggataa
    19381 tttttaccag aaaaatcact aattctttcg ttgctccaga cgacgcagag aacgctcacg
    19441 gcggctctct tcacgacttc tgtcgagcaa aatttcttcg ataaaggcca gatggcgatg
    19501 cgatgcttcg cgcgcttctt ccggcttacc ggccataatc gcttcaaata tgcgggtgcg
    19561 gtgactactc accagcggca gcatctcgcg acgcgaatag agcaattcga agttctggcg
    19621 gacattctgg gccaacatcg gctccataca ccttagcaga tgaagcagaa ccacattgtg
    19681 ggccgcttca gtgacagcaa tctgatactg gagtacggcg tttgattccg cgtccagatc
    19741 gccagactgc tgcgccagct ctatggcgtg gtggagttca cggatgcgtt ccttgtcttc
    19801 atcggtacta cgcagcgcgg cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc
    19861 gagcaagtca tactgtgact caggatggtc ggagagcagc tccaccagcg gatcgctgaa
    19921 gctttgccat aggctgctct ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc
    19981 cttcgcttcg agacgttgaa tcgcctcacg caaggaggga cgggagacgt caaactgttt
    20041 tgccagttcg cgttccggtg ggagtttttc gcccgggcgg agagtgcctt cgaggatcaa
    20101 aaactccagt tgctgctcaa tcacatcgga gagttttggt tggcggattt tgctgtaggc
    20161 catgagttcc tgtcttaagc cacttgccga agtcaattgg tcttaccaat ttcatgtctg
    20221 tgacgctaaa gtaacaaagt attcacctta tgtccataca ggttttgatt gaaatcatga
    20281 aactgtgcac attttaacaa cttgacatat ataacgtttc aaagttgtaa ctatgcacaa
    20341 atgtagactt tacg
//
LOCUS       DAZGOB010000085        19840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGOB010000085 DAZGOB010000000
VERSION     DAZGOB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.085"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(84..365)
                     /gene="yehE"
                     /locus_tag="ACHLIF_003284"
     CDS             complement(84..365)
                     /gene="yehE"
                     /locus_tag="ACHLIF_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO9607248.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(628..1737)
                     /gene="apbC"
                     /locus_tag="ACHLIF_003285"
     CDS             complement(628..1737)
                     /gene="apbC"
                     /locus_tag="ACHLIF_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO9607249.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1869..3902
                     /gene="metG"
                     /locus_tag="ACHLIF_003286"
     CDS             1869..3902
                     /gene="metG"
                     /locus_tag="ACHLIF_003286"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO9607250.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4043..7837
                     /locus_tag="ACHLIF_003287"
                     /pseudo
     CDS             4043..7837
                     /locus_tag="ACHLIF_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7847..11479
                     /gene="yehI"
                     /locus_tag="ACHLIF_003288"
     CDS             7847..11479
                     /gene="yehI"
                     /locus_tag="ACHLIF_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO9607251.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVIARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVHRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11541..11858
                     /gene="yehK"
                     /locus_tag="ACHLIF_003289"
     CDS             11541..11858
                     /gene="yehK"
                     /locus_tag="ACHLIF_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO9607252.1"
                     /translation="MIVQKELVAIYDYEIPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDTDPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            13098..14186
                     /gene="yehL"
                     /locus_tag="ACHLIF_003290"
     CDS             13098..14186
                     /gene="yehL"
                     /locus_tag="ACHLIF_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO9607253.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            14197..16476
                     /gene="yehM"
                     /locus_tag="ACHLIF_003291"
     CDS             14197..16476
                     /gene="yehM"
                     /locus_tag="ACHLIF_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO9607254.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIKGPADFNDRV
                     DELFLSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEANMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            16469..17605
                     /gene="yehP"
                     /locus_tag="ACHLIF_003292"
     CDS             16469..17605
                     /gene="yehP"
                     /locus_tag="ACHLIF_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO9607255.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            17602..19602
                     /gene="yehQ"
                     /locus_tag="ACHLIF_003293"
     CDS             17602..19602
                     /gene="yehQ"
                     /locus_tag="ACHLIF_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO9607256.1"
                     /translation="MNSLRPELLELAPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEGLATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            19727..>19840
                     /locus_tag="ACHLIF_003294"
     CDS             19727..>19840
                     /locus_tag="ACHLIF_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607257.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNG"
BASE COUNT         4961 a         5127 c         5031 g         4721 t
ORIGIN      
        1 attgttgata tcaataaaaa agccgaaatt atttatataa atgatttcgg ctttctaaat
       61 gcaattaaaa tcaggttgcc aaattaatcg tagcgattta acacgattcg acgtttgcta
      121 tcgctacctg caacaaccac ttctgtcgtt acacctttga cgggcgaaga aaacagatga
      181 cgggtgtcaa cattatacgt taactgacca caatgctgtt gaggttgacc attaaccata
      241 gccatactgc atgttggggg actgatcctg ccattaatcg ctaatgtggc agtctctgaa
      301 gagaatgcgg gagaagccaa tccataggca agaaaaataa ttcctgacag ccaatactta
      361 ttcattgaac gttatccctg tagtaaaggt tatgcctgga tagaatgagt gcataacaaa
      421 ctatagctgt acatccacta cacagccacg aaggatgata atgaagcatt gcctgtatga
      481 tcaatcgact ttgtagagtt tcggacgaag gtccgcagaa tattcgcagt attaaataag
      541 tcttcattaa tgcggcaaaa actaatacac cgcatcaatg taacatctct gcggtgtatg
      601 aagaataacc agaatggtta ataggcgtta gaccgcgcgg aaggaaatct cgcctggaat
      661 gacttcaccc tgccagtaga gctgagctgc aacgcggtca gccagctggc ggtagatagc
      721 ggtaaattcg ctctccggac ggctgataac cgttggcgtg cctttatcga gatcctcacg
      781 taaggagatg tgtaacggca tctgccccag cagctgtgtg tggtattttt cagccagttt
      841 ctcagcacca ccggtaccga aaatcggttc atggtgacca cagttactgc aaatatgcac
      901 actcatgttc tcgacaatac caagcaccgg aacctcgact ttctcgaaca tcacaatgcc
      961 ttttttcgca tcgatcaacg cgatgtcctg cggggtggtg accacgactg caccagttac
     1021 tggaatgttt tgcgccagcg tcaattgaat gtcaccggtg cctggcggca tatcaagcac
     1081 cagatagtcg agatccggcc acaaggtttc ctgcaacatc tgcattaacg ccttgctcgc
     1141 catcggtcca cgccacacca tcgcattgtc gtcagtgacc agataaccaa ttgaattggt
     1201 tgccaggcca tgagacataa tcggtgccat gtgggtaccg tccggcgacg ttggacgctg
     1261 attttctgcg cccagcatgg tgggaattga tgggccgtag atatcggcgt ccagaatgcc
     1321 gactttcgcc ccttccgccg ccagtgccag cgccaggttt accgccgtgg aggatttacc
     1381 cacgccgcct ttaccggaac tgaccgcaat gatgttcttc acaccattaa tacccggctg
     1441 attcttcacg cgttttaagg tggcgatatt gtgcgacagt ttccagtcga tagctttcgc
     1501 cccggtgata cgcagcagtt cggcgctaca ttgctctttt aactcttcaa acgcgctatg
     1561 ccagacgaat ggcataacca gttccacatg caacgtgtcg tccatccagg cgacatggtg
     1621 caacgctttc agcgtggtga ggttatgctt cagggttggg tgctgaaaat tggccagtgt
     1681 cccggcgacc attgcgcgca aggcttctgg tgatttggcc tgggattgtt cgttcatccc
     1741 gactcctttt ttgtatagat aaaccatcag ctgatagttt acctgaagaa tatagagaag
     1801 tacttactta acattttccc atttggtact atctaacccc ttttcactat taagaagtaa
     1861 tgcctactat gactcaagtc gcgaagaaaa ttctggtgac gtgcgcactg ccgtacgcta
     1921 acggctcaat ccacctcggc catatgctgg agcacatcca ggctgatgtc tgggtccgtt
     1981 accagcgaat gcgcggccac gaggtcaact tcatctgcgc cgacgatgcc cacggtacac
     2041 cgatcatgct gaaagctcag cagctcggta tcaccccgga gcagatgatt ggcgaaatga
     2101 gtcaggagca tcagactgat ttcgcaggct ttaacatcag ctatgacaac tatcactcga
     2161 cgcacagcga agagaaccgc cagttgtcag aacttatcta ctctcgcctg aaagaaaacg
     2221 gttttattaa aaaccgcacc atctctcagc tgtacgatcc ggaaaaaggc atgttcctgc
     2281 cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc cccggatcaa tacggcgata
     2341 actgcgaagt ctgcggcgcg acctacagcc cgactgaatt gatcgagccg aaatcggtgg
     2401 tttctggcgc tacgccggta atgcgtgatt ctgaacactt cttctttgat ctgccctctt
     2461 tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt gcaggagcag gtggcaaata
     2521 aaatgcagga gtggtttgaa tccggcctgc aacagtggga tatctcccgc gacgcgccat
     2581 actttggttt tgaaattccg aacgcgccgg gcaaatattt ctacgtctgg ctggacgcac
     2641 cgattggcta catgggttct ttcaagaatc tgtgcgacaa gcgcggcgac agcgtaagct
     2701 tcgatgaata ctggaagaaa gactccaccg ccgagctgta ccacttcatc ggtaaagata
     2761 ttgtttactt ccacagcctg ttctggcctg ccatgctgga aggcagcaac ttccgcaagc
     2821 cgaccaacct gtttgttcat ggctatgtga cggtgaacgg cgcaaagatg tccaagtctc
     2881 gcggcacctt tattaaagcc agcacctggc tgaatcattt tgacgctgac agcctgcgtt
     2941 actactacac tgcgaaactc tcttcgcgca ttgatgatat cgatctcaac ctggaggatt
     3001 tcgttcagcg tgtgaatgcc gatatcgtga acaaagtggt taacctggcc tctcgtaatg
     3061 cgggctttat caacaagcgt tttgacggcg tgctggcaag cgaactggct gacccgcagt
     3121 tgtacaaaac cttcactgat gccgctgaag tgattggtga agcgtgggaa agccgcgaat
     3181 ttggtaaagc agtgcgcgaa atcatggcgc tggctgatct ggctaaccgc tatgtcgacg
     3241 agcaggctcc gtgggtggtg gcaaaacagg aaggccgcga tgccgacctg caggcaattt
     3301 gctcaatggg cataaacctg ttccgcgtgc tgatgactta cctgaagccg gtactgccga
     3361 aactgaccga gcgcgcagaa gcattcctca atacagaact gacctgggat ggtatccagc
     3421 aaccgctgct gggccacaaa gtgaatccgt tcaaagcgct gtataaccgc atcgatatga
     3481 agcaggttga agcactggtg gaagcgtcta aagaagaagt gaaagcagct gccgcgccag
     3541 ttactggccc actggcagat gatccgattc aggaaaccat cacctttgac gacttcgcaa
     3601 aagttgacct gcgcgtggcg ctgatcgaaa acgcagagtt tgttgaaggt tctgacaaac
     3661 tgctgcgcct gacactggat ctcggcggtg aaaaacgcaa tgtcttctcc ggtattcgtt
     3721 ctgcctaccc ggatccgcag gcactgatcg ggcgtcacac cattatggtg gccaacctgg
     3781 caccgcgtaa aatgcgcttt ggtatctccg aaggcatggt aatggctgct ggtcctggcg
     3841 ggaaagatat tttcctgcta agcccggatg ccggtgctaa accgggtcat caggtgaaat
     3901 aatccccctt caaggcgctg catcgacagc gccttttctt tataaattcc taaagttgtt
     3961 ttcttgcgat tttgtctctc tctaacccgc ataaatactg gtagcatcag cattcaactg
     4021 gataaaatta cagggatgca gaatgaaaca ctttatctat caggacgaaa aatcacataa
     4081 attctgggca gttgagcaac agggaaacga gttgcatatc agttggggaa aagttggcac
     4141 caaagggcaa agccagataa aaagtttttc agatgctgcg gcagcggcaa aagcggagct
     4201 taagctgatt gcggagaagg tgaagaaggg gtatgtggag caagcgaagg ataattcttt
     4261 gcaaccttcc caaacggtaa cgggctctct caaggtagcg gacttatcca ccattattca
     4321 ggaacaaccc tctttcgtag cagaaacccg tgcgcctgac aaaaatacag atgctgtttt
     4381 accgtggctg gcgaaagata ttgctgtcgt ttttccgccc gaagttgtac acaccacgtt
     4441 aagtcatcgc cgctttcccg gagttcctgt tcagcaagca gacaaattga cccaattacg
     4501 tcgcttagcc tgtagtgtgt cgcaacggga taataaaaca gccacatttg acttcagcgc
     4561 ctgttcttta gaatggcaaa acaccgtcgc ccaggcgatc agtcagatcg acggcctgaa
     4621 aacaacacag ctaccatcac cagtaatggc tgtactcacg gcacttgaaa tgaaatgcac
     4681 aagatataaa gtgcgtgagg atgttatgga tcagatcgtc caggaaggcg gtctggaata
     4741 tgctactgat gtaataatac accttcaaca gattgatatt aaatgggatt atgcgaataa
     4801 tgtcattatt attctgccgt ctggcattgc acctgactac ttggagcaat attccagatt
     4861 tgaattacgc ctacgtaaac atttatcact ggcggaagag tctctctggc aaaaatgtgc
     4921 acaaaaactt attgccgcaa ttccacatat tccagaatga cggcaaccat taattgcttt
     4981 gttattacct gaaaaaccag aaattgcaca tgaaattgcc cagcgtctac tggggcaaaa
     5041 aaaattaccc tcgcttgagt ggttaaaaat agtggcgact gatgagcaca ttcttgcctc
     5101 attagaaaaa tatcacgagc catatgccat ttttgatgat tactattgtg gtgcgatatg
     5161 gtcagccacc gtattacagg agcaaggtgt tgcagccctg ccccgatttg ctccctatgc
     5221 cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat catccgttcg cattaacact
     5281 gcttatacgt gtagccgggc atactaaacg ctgtcacgat cggatgacga aagccattgc
     5341 tgcgttccca catgcagcaa tggcggcact gacggaactt cttgggcaaa aagaagagaa
     5401 cagttggcgc attatgctaa tgacaatgct tatctcacaa ccagcactgg cagaacaggt
     5461 cattccctgg ctctcgacac ccgcagttgc cgtactgaaa tcatgccagc aacaactgac
     5521 acagccctca aaccatgcca gcgccgatct actgccagcc gtagtagtct cccctccctg
     5581 gctttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcattga
     5641 gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta
     5701 ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg
     5761 ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg
     5821 gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg
     5881 gcattattgg cagttgggga ttttgacaac attgccgctg gagaaagcag taaaagcatg
     5941 gaatgccctt accctatctc cacataccga taccgaatac gccatgttac attttggact
     6001 caaagggtta cctgggttag taaactcact tgcacgctat ccacaagaag ccttgcccat
     6061 cacgaattac ttcgcagcga gtgagctggc tcctgccgtc gcccgtgcct tcaacaaact
     6121 gaaaacgcta cgcgaaaacg cccgtagctg gctgttgaaa tacccggaac atgcccttac
     6181 cggcctgctg cctgcggcgc tcggcaaagc cggtgaagca caggataacg cccgcgctgc
     6241 cttgcgtatg cttaccgaaa acggtcatca gccattactg caagaaatcg cccgacgtta
     6301 taaccagccg gaagtaaccg atgcggtgaa cgctctgctt gcgctcgatc ccttagataa
     6361 tcacccgaca aaaatcccca ctcttccggc cttttatcag ccatcgctct ggacgcgccc
     6421 ggtattaaaa gcaaatgccc aatcactgcc agatagcgcc ctcctccacc tcggtgaaat
     6481 gctccgcttc cctcaggaag aggctctgta tccgggatta ttgcaggtga aagacgtctg
     6541 ttccgccgac tcactggcgg gatttgcctg ggatctgttt accgcctggc agaccgctgg
     6601 cgcgccgtcg aaagagagtt gggcgttcac tgcgttaggc gttctcggta acgatgacac
     6661 cgcccgcaaa ctgacgccat taatacgcgc ctggcctggt gaatcccagc ataaacgcgc
     6721 caccgttggg ttggatattc tcgctgctat cggtagtgat atcgccctta tgcagcttaa
     6781 cggcatcgcc cagaaactga aattcaaagc attacaggag cgggcaaaag aaaaaattgc
     6841 cgacattgcc gagagccgcg aactcacggt ggcggagctt gaagatcggt tagcaccgga
     6901 tctcggtctg gatgataacg gttcgctgct gctggatttt ggcccacggc agttcaccgt
     6961 cagctttgat gaaaccttaa aaccgtttgt gcgtgatgtt tccggcagcc gcctgaaaga
     7021 cctgcccaaa ccgaacaaaa gcgatgatga atcgcggtcg aacgatgcgg ttaaccgcta
     7081 caaattgctg aaaaaagatg cgcgtaccgt cgccgcccag caggtagcaa ggctggaatc
     7141 cgccatgtgc ctgcgccgcc gctggtcgcc ggaaaacttc cagctcttcc tggttgagca
     7201 tccgctggtt cgccacttaa cccgccgtct gatttggggc gtttatagcg ccgaaaacca
     7261 gctactggct tgctttcgcg tagcagaaga taacagctac agcaccgctg acgatgatct
     7321 tttcaccctg ccggaaggcg atatctctat cggcattcct cacgttctgg agatatcacc
     7381 gacggatgcc gccgcctttg ggcagctttt tgccgactat gaactgttac cgccgttccg
     7441 ccagctcgac cgtaacagtt acgccctgac agaaaccgag cgcaatgcca gcgaattgac
     7501 ccgctgggca ggcagaaaat gcccgagcgg tcgggtcatg ggactggcga ataaaggttg
     7561 ggtgcgtggt gaaccgcagg atggtggctg gatcggctgg atgatcaaac ctctgggccg
     7621 ctggtcgtta atcatggaaa tcgatgaagg ctttgcagtt ggtatgtcgc cagccgaact
     7681 tagcgccgag caactcttaa gcaagctgtg gctatgggaa ggcaaagcag aaagctatgg
     7741 ctgggggagt aattcaacac aggaagcgca gttctccgta ctcgatgcca tcaccgccag
     7801 cgagctaatt aacgatattg aagccctgtt tgaataagga aagagcatgg acaaggaatt
     7861 accgtggctg gcggataacg cccaactgga actgaaatat aaaaaaggca aaacgccgct
     7921 cagtcatcgt cgctggccgg gcgaaccagt gtccgttatc actggaagtc tcatccagac
     7981 attgggagat gaattactac aacaagcagg ccagaaggaa aatatcacct ggaattatga
     8041 taaatgctca ctggagtggc agtccgccat ccagcaggca atcaatttga ctggcgagca
     8101 taaaccgtca atcccagccc ttaccatggc ggctctgatc tgtatcgcgc aaaatgacag
     8161 ccagcagtta cttgatgaaa tcgtgcaaca agaggggctg gaatatgcga cggatgtagt
     8221 gatagcgcgt cagtgcattg cgcgacgtta tgagagtgac tcactggtcg tcactttgca
     8281 gtatcaggac gaggactatg gctacggtta tggctccgcg acctataacg atttcgatct
     8341 ccgtttgcgt aagcatctgt cgctggcaga ggaaagttgc tggcagcgtt gtgccgataa
     8401 actcattgcc gcactgccag gaatacccaa aattcgccgt ccttttattg cgctgatcct
     8461 cccggaaaaa cctgaaatag ccaatgaact cgccagtctt gaaagctctc ggtcaagtct
     8521 ccattcaaag gagtggttaa aggtcgttgc tactgataat acggcggtaa aaaaactcga
     8581 acgatactgg ggcctggacg tatttagcga tcgcgaagcc agctacatgt cacaagaaaa
     8641 ccgcttcggc tacgcggcct gcgcctccct tttgcgcgaa caaggactgg cagccgttcc
     8701 gcgcctggca atgtatgccc ataaagaaga ttgcggcagt ctgctggtac aaattaacca
     8761 tccgcaagtc atccgcacct tgctactggt ggctgataaa aacaaaccca gcctgcaacg
     8821 tgtggctaaa tacagtaaaa acttccccca cgcgacgctc gccgcactgg cagaactgct
     8881 ggcgttaaaa gaaccaccag cccgcccagg ttatccaatc atcgaagaca aaaagctgcc
     8941 tgcacagcaa aaagcacgcg atgaatactg gcgtacgctg ttacaaacgc tgatggcatc
     9001 gcagccacaa ctggcagcag aagtgatgcc gtggttaagt actcaagcca gggcagtgct
     9061 gaatagttat ttatcggcac cgccaaaacc ggttattgat agcaccgata acagcagtct
     9121 gcctgaaatg ctcgtttcac caccgtggcg tagtaagaaa aaaatgacag ctccacgtct
     9181 tgatttggca ccgctcgaat taactccgca aatttactgg caaccaggcg aacaagagag
     9241 gcttgccgcc actgagtctg cccgttattt cagcacggaa tctcttgcgg aacgcatgga
     9301 acaaaaaagt ggacgagttg tattacagga actgggtttt ggggatgatg tatggctgtt
     9361 tctgaattat atactccccg gaaaactgga tgctgcacgc aattcactca ttgttcagtg
     9421 gcattactac caggggcggg ttgaagagat cctgaatggc tggaactccc cgcaagcaca
     9481 attagcagaa caggcgctcc gcagcggtca catagaagcg ttaattaaca tatgggaaaa
     9541 tgacaacttc tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt tattggcaca
     9601 gttgccgcgt gagatggctt tgaccttctg gctgcgtatc attgagaaaa agcatctgtt
     9661 cgcgggtgag gactattttc tcagtatcct cggattggat gcgctaccag gtctgctgtt
     9721 ggctttttca catcgtccaa aagaaacatt tccgttaatt ttaaattttg gcgcaacaga
     9781 actggcgctg cctgttgccc gcgtctggcg acgtttcgcg gcgcagcgtg atctggctcg
     9841 ccagtggatt ttacaatggc cggaacatac ggctagtgca cttatccctc ttgtctttac
     9901 caaacccagc gataatagcg aagccgcatt acttgccctg cgtttactgt acgaacaggg
     9961 acatggcgaa ttgctacaaa ccgtggcaaa ccgctggcag cgtacagatg tatggtctgc
    10021 cctggagcag ttgcttaaac agggtccaat ggacatttac ccggcacgca ttccaaaagc
    10081 ccctgatttc tggcatccgg caatgtggtc caggcctcga cttatcacta ataatcagcc
    10141 tgttaccggt gacgctctgg aaattatcgg cgaaatgctg cgctttaccc aggggggacg
    10201 tttttatagc gggctggaac aactgaaaac gttctgccag ccacaaacgc tggcagcttt
    10261 tgcctgggat ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag acaactgggc
    10321 atttctggcg ttaagtctct ttggtgacga aagcacggca cgggatctga cgacacagat
    10381 cctcgcctgg ccacaagaag gcaaatctgc ccgtgctgtc agcggcctga acatccttac
    10441 cctgatgaat aatgatatgg cgctgataca gctgcatcat atatcgcaac gggctaaatc
    10501 ccgcccctta cgtgataacg cggcggaatt tcttcaggta gtcgcagaaa atcgcgggct
    10561 aagccaggaa gagctagcgg acagattagt cccaaccctg ggccttgatg atccgcaggc
    10621 gttgagtttt gattttggtc cccggcagtt taccgttcgc ttcgatgaaa acctcaaccc
    10681 ggttatcttt aatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc gcgccgatga
    10741 cgatcaactg aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa aagatgctac
    10801 tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca cccgacgctg
    10861 gtcgctggca gattttcatt ctctgtttgt taatcatccc tttacccgtc tggttaccca
    10921 gcgattaata tggggggttt atccggcaaa tgaaccgcgt tgtttactca aagcctttcg
    10981 tgtggccgca gagggggagt tctgcaatgc gcaagatgag ccaattgacc tgcctgcgga
    11041 cgctctgatt ggcattgccc acccgttaga aatgacagta gaaatgcgca gtgaatttgc
    11101 acagcttttt gccgattacg aaattatgcc gccttttcgc cagttgtcgc gccgcacggt
    11161 gctgctcaca cctgacgagt caaccagtaa cagcctgact cgctgggaag gtaaatccgc
    11221 taccgttggg cagcttatgg gaatgcgata caaaggctgg gagtcaggtt atgaggacgc
    11281 atttgtctat gacctgggtg agtaccggct ggtccttaag ttttcacccg gttttaacca
    11341 ctacaatgtt gatagcaaag cgctaatgag cttccgttct cttcgagtgc accgtgacaa
    11401 taaatccgtc acttttgccg aacttgatgt gtttgatttg agtgaggcgt taagcgcacc
    11461 tgacgtcatt ttccattaaa aacacagcgg gcagtgatgc aactgcccgt tatcaacacg
    11521 acttaccagc ggggaaagcg atgatcgtgc aaaaagagct ggttgctatt tacgattatg
    11581 agatccctgt acctgaaaat ccgttttcct tcagacttga gatccataaa tgctctgaat
    11641 tatttacagg ttccgtctat cgactggagc gattccggct acgtccaaca tttcatcaac
    11701 gtgatcgaga agatactgac ccgctaataa atgatgcgtt gatttatata agagatgagt
    11761 gtattgatga gcggaaatta cgaggtgaat cacctgaaac tgtaatagca atttttaatc
    11821 gtgaactaca gaatatattc aaccaagaaa tagaataata tactctagat aattaaagtc
    11881 gcagcacatg caacttgaag tatgacgagt atagctttct taaaggggga aacggtattc
    11941 ccccttagat gcgtttcaat ttgcccgtta cttcaacaaa tcaatcaacc gtaacagagc
    12001 cactaataac tccagtacgg caatgagcac ggttaacttt tgtgtcagcg tgtccatccg
    12061 ctctccttat taaggagcgc aacaccagca ccttaccctt acactgcctg ttgatactgg
    12121 tttacgtatg ttggtacgaa acgttgctct ccggatcagg gcaccgcctg cggcaccacc
    12181 cgtccggcag ccaggactta aaattggtcc gatccggcac aacctcccaa cgggcaggat
    12241 tataaatctc actcactgaa ttgcaatgga gtgtggatat ggttataatt tattcgttgg
    12301 tacgaaatgt tgcacaatca gcaagttagc gtcactgtag taaataaaag aacctgcctc
    12361 accagcaggt ttttttattt tctgcgatct actttccaga tattttttgc caatagccca
    12421 acataaatat tcacatcccg gtcaaatggt gcgttgaata taaaatcgat gaatttataa
    12481 ataaaaatga gttatatgat gaattaataa aaacagtaat cattttatta aagggggaag
    12541 cggttttccc ccttagatgc gtttcaattt gcccgttact tcaacaaatc aatcaaccgt
    12601 aacagagcca ctaatagctc cagtacagca atgagcacgg ttaacttttg tgtcagcgag
    12661 tccatccgct ctccttatta aggagcgcaa caccagcacc ttacccttac actgcctgtc
    12721 gatactggtt tacgtatgtt ggaccacgat gttgcactcc ggatcagggc accacctgca
    12781 gcaccccccg tccggtagcc aggacttgaa ataggttcga tccggcgcaa cgtcccaaca
    12841 gtcaggatta taaatctcat tatcttaatt gcaacggggt ccagccgtgg ttataattcg
    12901 ttcgttggaa cacgatgttg cacaggctgt ggtgttaggc ctgaaaatag taaataaaag
    12961 aacctgcctc accagcaggt ttttttattt actgtgatct gctttccaga tattttccgc
    13021 tcaaacaact aatgcgccaa acatttattg cgcgtaaaat atcgtttatt tcattaatac
    13081 atttcaggga tgaatatatg tcaccgcaga ataatcatct gcagcgtccg cctgctgctg
    13141 tgttatacgc cgatgaactg gcaaaattaa aacaaaatga taacgcacct tgcccgcccg
    13201 gttggcagtt aagtttgcct gcggcccgtg cttttatcct tggcgatagc gcgcaaaata
    13261 ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg tatgttagtc actcttgcca
    13321 ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc aaaatctctc ctgtctgaat
    13381 tactggcaac cgctattagc ggcgatgccg ggttaaccat ccagggcggc gcatctacca
    13441 ctgaagatca aatcaagtac ggctggaact acgctctgct tatcaatcat ggcccatcaa
    13501 cggaagcgct ggtccctgcc ccgctttacc agggaatgcg cgacggcaaa atcgtccgtt
    13561 tcgaagagat aacgcgtaca ccgctggaag tgcaagattg tctgttaggt atgctttctg
    13621 acagagttat gacggtgccg gaactcactg gtgaagccag tcaactctat gcgcgggaag
    13681 gctttaatat tattgccact gccaataccc gcgatcgcgg cgtcaatgag atgagtgccg
    13741 cgctaaagcg tcgcttcgat tttgaaactg tctttccgat tatggatttc gctcaggagc
    13801 tggagcttgt cgccagtgct tcggcgcgtt tgctggccca tagcggtatt ccgcataaag
    13861 tgcctgacgc ggtactggag ttactggtcc gcaccttccg cgatctgcgt gccaacggcg
    13921 aaaagaaaac gtcaatggat acactaacag cgattatgtc caccgctgaa gccgtgaacg
    13981 tggcccacgc cgtaggcgtc cgcgcctggt ttttagcgaa tcgcgcaggt gagccagccg
    14041 acctggtgga ctgcattgcc ggaaccatcg tcaaagataa cgaagaagat cgcgctcgtc
    14101 tgcgccgtta cttcgaacaa cgagtcgcca cacataaaga agctcactgg caggcttatt
    14161 atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga gcgagccgtt aattgtcggc
    14221 atccggcatc atagtccggc ctgcgcccgg ctggtgaaat cgttaatcga aagccagcgg
    14281 ccacgatacg tgttgattaa aggcccggct gattttaatg accgggtaga cgaactgttt
    14341 ttatcccacc agcttccggt agctatttac agttattgcc agtatcagga cggtgcagcc
    14401 cccgggcgtg gtgcctggac gccatttgct gaattttcgc cggagtggca ggcgctacaa
    14461 gccgcacgtc gtattcaggc acaaacttac ttcatcgatt tgccttgctg ggcgcagagt
    14521 gaagaagagg acgattcgcc tgatacgcaa gatgaaagcc aggccttact gctgcgtgcc
    14581 acccgcatgg ataacagcga taccctgtgg gatcacttgt tcgaagatga aagccagcaa
    14641 actgcattac cctctgcgct ggcgcactat tttgcccaac tgcggggcga tttccccggc
    14701 gatgcactca atcgtcagcg cgaagccttt atggctcgct ggattgcatg ggcggtgcag
    14761 caaaataatg gcgacgtgtt agtcgtctgc ggtggctggc acgctccggc actggcaaaa
    14821 atgtggcgcg aatgcccgca ggacattaac acgccggaat tgccctcgct ggcagatgcc
    14881 attacaggtt gttatctcac gccctacagt gaaaagcgcc ttgatgtgct ggcaggatac
    14941 ctttccggta tgcctgcccc ggtctggcaa aactggtgct ggcagtgggg cttacagcag
    15001 gccggtgaac aactgctaaa aactatcctt acccgtttgc gccagcacaa gttgcctgct
    15061 tcgaccgcgg atatggctgc cgctcatctg catgcgatgg cgctggcaca gttgcgcggt
    15121 catacactac cgttacgcac tgactggctg gatgccatag caggctcgct gattaaagaa
    15181 gccctgaatg cgccgttgcc gtggagctat cgcggcgtta ttcatcccga taccgatccg
    15241 attctgctaa cgttgataga cacattagcg ggtgacggat tcggtaaact tgccccttct
    15301 acgccacaac cgcctctgcc aaaagatgtc acctgcgaac tggaacgtac cgcaatctct
    15361 cttccggcgg agcttacctt aaatcgcttt aaccccaatg gactagcgca aagtcaggtg
    15421 ttacatcggc tggcaatact ggagatccca gggattgtac gccagcaggg aagcacactg
    15481 acacttgcag gcaacggtga agaacgctgg aaattaaccc gcccgcttag ccagcatgcg
    15541 gcattgattg aggccgcctg ctttggtgca acactccagg aagccgcacg ccataaatta
    15601 gaagccaata tgctggacgc gggtggaatc ggcagtatca ccacatgtct tagccaggcg
    15661 gcgttagcgg gtctggcgtc cttcagtcaa caattactgg agcaactcac attattaatc
    15721 gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg aagtgctata tgccttatgg
    15781 cggctggatg aaattagcgg tatgcaaggc gcgcagatat tacaaacgac gttatgcgcg
    15841 gctatcgatc gcacgctgtg gctatgtgaa tctaacggca gaccagatga aaaggagttt
    15901 cacgctcacc tgcatagctg gcaagcgttg tgtcatatcc tgcgcgatct acatagcggt
    15961 gtgcaactcc ccggcatttc tctttctgcg gcggtagcct tactggagcg acgcagtcag
    16021 gcaattcatg ccccggcgct ggatcgcggc gcggctcatg gcgcactgat gcgtctggaa
    16081 catcctaacg ccagtgccga agcggcgctg acgatgctgg cgcagttatc cccggcacaa
    16141 tccggcgaag cgctgcacgg tttgctggcg ctggcccgcc atcaactggc ctgtcagccg
    16201 acatttatcg ccggtttcag cagtcattta aatcaactaa gtgatgccga ttttatcaat
    16261 gccctgcccg atttacgcgc ggcgatggcc tggctaccac cacgagaacg cgggacgctg
    16321 gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc ccgtttcggc gctgcaaatg
    16381 ccgttgcatt gtccaccaca ggccattgca catcatcaac aactcgaaca gcaggcactg
    16441 gcatcgctgc aaaactgggg agttttccat gtctgaactg aacgatcttc tgaccacccg
    16501 tgagctacaa cgctggcgat taattcttgg cgaagcggca gaaacgacgc tttgtgggct
    16561 ggatgacaac gcccggcaga tagaccacgc gctggagtgg ctgtatgggc gcgatcctga
    16621 acggctccag cgtggtgaac gctctggtgg attaggtggc tcaaatctca ccacccctga
    16681 gtggatcaac agtattcaca ctctgtttcc gcaacaggtg attgagcgac tggaaagcga
    16741 tgccgtactg cgctacggca ttgaagatgt ggtgacgaat ctcgacgtgc tggaacgtat
    16801 gcagccttcg gaaagcctgc tacgcgccgt tttgcacacc aaacatctga tgaatcccga
    16861 agtactggct gccgcccgcc agatagtgcg ccaggttgtt gaagaaatta tggcgcgact
    16921 ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat cgccgtcgcc gctcattaat
    16981 tccactggcg cgaaactttg atttcaaaag tactctgcgc gccaacctgc aacactggca
    17041 cccgcaacac ggcaagttgt atatcgaatc cccccgcttt aacagccgca ttaaacgcca
    17101 aagcgaacaa tggcaactgg tcttactggt tgatcaaagc ggatcgatgg tggactcggt
    17161 gatccactct gcggtgatgg cggcctgttt gtggcagtta cccggcattc gtacccatct
    17221 ggtggcgttt gacacaagcg tggttgatct cacggcagac gttgccgatc cggtagagtt
    17281 attaatgaaa gtacagttgg gcggcgggac caatatcgcc agtgccgtgg agtatggtcg
    17341 gcaacttatt gaacaaccag caaaaagcgt cattatcctc gtgagcgatt tttacgaagg
    17401 gggttcatca tcattgctga cgcatcaggt gaaaaagtgt gtccagagcg gcatcaaagt
    17461 gctgggactg gcagcgctcg atagcaccgc aacgccttgc tatgaccgcg atacggccca
    17521 ggcgctggtt aatgtcggcg cacaaatagc cgccatgacg ccgggcgagc tggcatcatg
    17581 gcttgcggag aatcttcagt catgaattca ctacgtccgg aattattaga actggcaccg
    17641 caggcactga cggcgttaag caatgccggt tttgttaagc gcagtcttaa ggaactggaa
    17701 aatggcaacg tcccggaaat cagccatgag aacggcgctt taatcgccac cttcagtgac
    17761 ggtgtccgta cccagctggc gaacagccag gcactgaaag aggctcagtg cagctgcggg
    17821 gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa gttatcaacg actttgtgcc
    17881 accgtccagc ccacggaaaa agaagaagag tgggatccgg caatctggct ggaaggactg
    17941 gctaccctgc ccgatgccac ccgcaaacgc gcgcaggcgc tggtcgctaa aggcatcacc
    18001 attgagttgt tctgtgcgcc gggcgaaatt ccctctgccc gcttaccgat gagtgatgtg
    18061 cgtttttatt cccgtagcag tattcgtttc gcccgttgtg attgtattga aggcacactt
    18121 tgcgaacatg tcgtactggc tgtgcaggcc ttcgtcgagg ccaaagcgca gcaagcagaa
    18181 tttaaccatt taatctggca gatgcgcagc gaacacgtca catcatctga cgatccgttt
    18241 gccagcgaag aaggccaaac gtgtcgtcaa tatgttcagc aattaagcca ggcattatgg
    18301 cttggcggca tcagccagcc gcttattcac tacgaggcag cattcaaccg cgcattgcag
    18361 gcggcagagg cctgcaactg gcgctgggtg agtgaatcac tacgacagct acgggcaagc
    18421 gttgatgcct tccacactcg cgccagccac tatcatgccg gagaatgctt acgtcagctt
    18481 gcggcattaa acagtcgatt aaattgcgca caagagatgg cccggcgcga cagtgttggt
    18541 gaagttcctc ctgtgccgtg gcgcacggtc gttggctctg gcattgccgg agaagcaaag
    18601 cttgatcatc tgcggctggt gtctttaggt atgcgttgct ggcaggatat tgagcattat
    18661 ggtttacgca tctggtttac cgatcccgac accggcagta ttttgcacct ttcgcgcagt
    18721 tggccgcgaa gtgaacagga aaactcaccg gcagctacgc gtcggctgtt tagttttcag
    18781 gctggcgcac tggcgggtgg gcaaattgtt tcacaagcag caaaacgcag tgccgatggc
    18841 gagctgctgt tagctacccg caaccgctta agcagcgttg tgccgctgtc gcctgatgcc
    18901 tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg tggctttgcg ggaatattta
    18961 cgccagcgtc cccccgcctg catacggcct cttaatcagg tcgataactt atttattctg
    19021 ccggtcgctg agtgtatttc gctcggttgg gatagcagcc gccagacgct ggatgcgcag
    19081 gtaatcagcg gcgaaggtga ggataatctc ctgacgttat cccttccggc atctgcctgt
    19141 tctccttttg ccgttgaacg catggcggcg cttttgcaac aaacagacga ccccgtgagt
    19201 ctggtttctg gctttgtcag ttttgttgat gggcaattga cactggaacc acgggtgatg
    19261 atgacaaaaa cccgcgcctg ggcgctggac gcggaaactg cgcctgtggc accgctacct
    19321 tctgccagcg ttttgcctgt gccgtctacc gctcatcagt tgctgatgcg ctgccaggcg
    19381 ttacttattc aactgctcca taacggctgg cgctatcagg aacagagcgc tattagtcag
    19441 gcagagttgc tggcgaatga cctctccgcg gtcggttttt atcggctggc acatgtgttg
    19501 ggacaatttc gtaatacaga aagcgaggca cgggtagaag caatgaataa cggtgtttta
    19561 ctttgcgaac aattattccc catgcttcag caacaaggat gaaatagtgc tttttactaa
    19621 gagttctact ccagttccgg actgctcacg tcacggtatt aggcatattc tatatagccc
    19681 ctggtgagag tcaccagttt cttgattaaa taaaatggag ttttacatga aggctttcaa
    19741 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
    19801 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc
//
LOCUS       DAZGOB010000086        19592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGOB010000086 DAZGOB010000000
VERSION     DAZGOB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.086"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            242..892
                     /gene="csgD"
                     /locus_tag="ACHLIF_003295"
     CDS             242..892
                     /gene="csgD"
                     /locus_tag="ACHLIF_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO9607258.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            897..1286
                     /gene="csgE"
                     /locus_tag="ACHLIF_003296"
     CDS             897..1286
                     /gene="csgE"
                     /locus_tag="ACHLIF_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO9607259.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1311..1727
                     /gene="csgF"
                     /locus_tag="ACHLIF_003297"
     CDS             1311..1727
                     /gene="csgF"
                     /locus_tag="ACHLIF_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO9607260.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1754..2587
                     /gene="csgG"
                     /locus_tag="ACHLIF_003298"
     CDS             1754..2587
                     /gene="csgG"
                     /locus_tag="ACHLIF_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO9607261.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2651..3142)
                     /gene="ycdZ"
                     /locus_tag="ACHLIF_003299"
     CDS             complement(2651..3142)
                     /gene="ycdZ"
                     /locus_tag="ACHLIF_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO9607262.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGSFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3244..3798)
                     /gene="ycdY"
                     /locus_tag="ACHLIF_003300"
     CDS             complement(3244..3798)
                     /gene="ycdY"
                     /locus_tag="ACHLIF_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO9607263.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3822..4559)
                     /gene="ycdX"
                     /locus_tag="ACHLIF_003301"
     CDS             complement(3822..4559)
                     /gene="ycdX"
                     /locus_tag="ACHLIF_003301"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO9607264.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4614..5552)
                     /gene="ghrA"
                     /locus_tag="ACHLIF_003302"
     CDS             complement(4614..5552)
                     /gene="ghrA"
                     /locus_tag="ACHLIF_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO9607265.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGADVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5786..5873
                     /locus_tag="ACHLIF_003303"
     tRNA            5786..5873
                     /locus_tag="ACHLIF_003303"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5820..5822,aa:Ser,seq:gga)
     gene            complement(6023..6865)
                     /locus_tag="ACHLIF_003304"
     CDS             complement(6023..6865)
                     /locus_tag="ACHLIF_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9607266.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6950..7147)
                     /locus_tag="ACHLIF_003305"
     CDS             complement(6950..7147)
                     /locus_tag="ACHLIF_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9607267.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7159..7650)
                     /locus_tag="ACHLIF_003306"
     CDS             complement(7159..7650)
                     /locus_tag="ACHLIF_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9607268.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7647..8021)
                     /locus_tag="ACHLIF_003307"
     CDS             complement(7647..8021)
                     /locus_tag="ACHLIF_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9607269.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(8068..8442)
                     /locus_tag="ACHLIF_003308"
     CDS             complement(8068..8442)
                     /locus_tag="ACHLIF_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9607270.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8605..8826)
                     /locus_tag="ACHLIF_003309"
     CDS             complement(8605..8826)
                     /locus_tag="ACHLIF_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9607271.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8889..9365)
                     /locus_tag="ACHLIF_003310"
     CDS             complement(8889..9365)
                     /locus_tag="ACHLIF_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO9607272.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9381..9866)
                     /locus_tag="ACHLIF_003311"
     CDS             complement(9381..9866)
                     /locus_tag="ACHLIF_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9607273.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9957..10775)
                     /locus_tag="ACHLIF_003312"
     CDS             complement(9957..10775)
                     /locus_tag="ACHLIF_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9607274.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(11115..12185)
                     /locus_tag="ACHLIF_003313"
     CDS             complement(11115..12185)
                     /locus_tag="ACHLIF_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO9607275.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALWEHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            complement(12182..13087)
                     /locus_tag="ACHLIF_003314"
     CDS             complement(12182..13087)
                     /locus_tag="ACHLIF_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO9607276.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            complement(13084..15468)
                     /locus_tag="ACHLIF_003315"
     CDS             complement(13084..15468)
                     /locus_tag="ACHLIF_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO9607277.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGW
                     ENYDEDVSRSVININKAREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            complement(15579..18428)
                     /gene="cah"
                     /locus_tag="ACHLIF_003316"
     CDS             complement(15579..18428)
                     /gene="cah"
                     /locus_tag="ACHLIF_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO9607278.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            complement(18800..>19592)
                     /locus_tag="ACHLIF_003317"
     CDS             complement(18800..>19592)
                     /locus_tag="ACHLIF_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9607279.1"
                     /translation="HLRKLTSHEPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGT
                     REVRRFRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRAL
                     SVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQEQNIREKTDAVFR
                     LFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELCTESVRGQAREQ
                     FTGAVDRIFDTVESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT         4602 a         5250 c         4879 g         4861 t
ORIGIN      
        1 ataaaacctt aagattaaca ttttaatata acgagttaca tttagttaca tgtttaacac
       61 ttgatttaag atttgtaatg gctagattga aatcagatgt aatccattag ttttatattt
      121 tacccattta gggctgattt attactacac acagcagtgc aacatctgtc agtacttctg
      181 gtgcttctat tttagaggca gctgtcaggt gtgcgatcaa taaaaaaagc ggggtttcat
      241 catgtttaat gaagtccata gtattcatgg tcatacatta ttgttgatca ctaaaccgtc
      301 tttgcaagcg acagctctct tgcagcacct taaacaatcg ctggcaatta caggaaaatt
      361 acataatatt caacgttctc tggacgatat atcttcaggc tctattattc ttctggatat
      421 gatggaagca gataaaaagc ttatccatta ttggcaggat actttgagca ggaaaaacaa
      481 caatatcaaa atattgttgc taaatacgcc tgaagattac ccgtaccgcg acattgaaaa
      541 ctggcctcat atcaacggcg ttttttatgc catggaggat caagaacgtg ttgtcaatgg
      601 gttgcaaggc gtcctgcgcg gcgaatgcta ctttacgcaa aagcttgcca gttacctgat
      661 tacacattct ggtaactatc gttataacag cacggaatca gccctcctta ctcatcggga
      721 aaaagagatc ctgaataagc tgcgtatcgg cgcgtctaat aacgagatcg ctcgttcgtt
      781 gttcatcagc gaaaatacgg ttaaaacgca tctttataat cttttcaaga agatagccgt
      841 aaaaaaccgg acacaagcgg tttcctgggc aaacgataac ctcaggcgat aaagccatga
      901 aacgttattt acgctggatt gtggcggcag aatttctgtt cgccgcaggg aatcttcacg
      961 ccgttgaggt agaagtcccg ggattgttaa ctgaccatac tgtttcatct attggccatg
     1021 atttttaccg agcctttagt gataaatggg aaagtgacta tacgggtaac ttaacgatta
     1081 atgaaaggcc cagtgcacga tggggaagct ggatcactat aacggtcaat caggacgtta
     1141 ttttccagac ttttttattt ccgttgaaaa gagacttcga gaaaactgtc gtctttgcac
     1201 tgattcaaac tgaagaagca ctaaatcgtc gccagataaa tcaggcgtta ttaagtacgg
     1261 gcgatttggc gcatgatgaa ttctaaataa aaaattgttc ggaggctgca atgcgtgtca
     1321 aacatgcagt agttctactc atgcttattt cgccattaag ttgggctgga accatgactt
     1381 tccagttccg taatccaaac tttggtggta acccaaataa tggcgctttt ttattaaata
     1441 gcgctcaggc ccaaaactct tataaagatc cgagctataa cgatgacttt ggtattgaaa
     1501 caccctcagc gttagataac tttactcagg ccatccagtc acaaatttta ggtgggctac
     1561 tgtcgaatat taataccggt aaaccgggcc gcatggtgac caacgattat attgttgata
     1621 ttgctaaccg cgatggtcaa ttgcagttga acgtgacaga tcgtaaaacc ggacaaacct
     1681 cgaccatcca ggtttcgggt ttacaaaata actcaaccga tttttaagcc ccagcttcat
     1741 aaggaaaata atcatgcagc gcttatttct tttggttgcc gtcatgttac tgagcggatg
     1801 cttaaccgcc ccgcctaaag aagccgccag accgacatta atgcctcgag ctcagagcta
     1861 caaagatttg acccatctgc cagcgccgac gggtaagatc tttgtttcgg tatacaacat
     1921 tcaggacgaa accgggcaat ttaaacccta cccggcaagt aacttctcca ctgctgttcc
     1981 gcaaagcgcc acggcaatgc tggtcacggc attgaaagat tctcgctggt ttataccgct
     2041 ggagcgccag ggcttacaaa acctgcttaa cgagcgcaag attattcgtg cggcacaaga
     2101 aaacggcacg gttgccatta ataaccgaat cccgctgcaa tctttaacgg cggcaaatat
     2161 tatggttgaa ggttcgatta tcggttatga aagcaacgtt aaatcgggcg gtgttggggc
     2221 aagatatttt gggatcggtg cagacacgca ataccagctc gatcagattg ccgtgaacct
     2281 gcgcgtcgtc aatgtgagta ccggtgagat cctctcctcg gtgaacacca gtaagacgat
     2341 actttcctat gaagttcagg ccggggtttt ccgctttatt gactaccagc gcttgctgga
     2401 aggggaagtg ggttacacct caaacgaacc cgttatgctg tgtctgatgt cggctatcga
     2461 aacaggggtc attttcctga ttaatgatgg tatcgaccgt ggtctgtggg atttgcagaa
     2521 caaagcagaa cggcagaacg acattctggt gaaataccgc catatgtcgg ttccaccgga
     2581 atcctgacag ataaaaaagc cggaattatc atccggcatt ttactgttta agcgaaagcg
     2641 tgagtgcccc tcacgcttta tttttgattt cctgctcacg gtgagccgtc tttgcactac
     2701 gcgccgccag ccacaggccg ctgtttttca ttgcgtaacc aaataccagc ccaagcgcca
     2761 gcgaaggtaa caccagtttc cagtcaccct gcccggcaaa cgtcgcgcat gcgcctataa
     2821 acgaccccgg aacaaatgaa agcagcagct gtttggcctg aatacacatc agaaacgcga
     2881 caataccggt tatgacataa ccgagaattt ccagatgtgg tgccagtgca ctaccgtaaa
     2941 taatgaccat cgcccacacc acgccactaa gcagggttgc agcggagatc gccagccctt
     3001 tcagcccacc ttgcgggcag gcaaagtagg ccgtacagcc caggaagccc gcccagctta
     3061 gtaagcccag ggaaacagcc acccatcccc agataccgga gagaatgcct gttgtgattg
     3121 caatagagag aagtatgttc atgccgcgca tgatagcaga aaacgcggcc atgaatgaga
     3181 tcgagcacac attttatgca acaacaaatt atctgttgca gttatgagtg acgatgatca
     3241 catttactct tcagaatctt cttccagctc gtcccacatt gcactaatgg catcacgggt
     3301 tagcggtgcc atggtgcgcc agaaaggcgt ggttgcatgg gcctccactt tgccgaggaa
     3361 cgcaccacac cagggtaaca gatactcact gaacagtgtt tccagtgctt cgctctcatc
     3421 ttccgttgac tgatcttcca gccaggaagc cgcaagcagc aatgtgccga tgtgatcggc
     3481 tggcgtatcc gccagtggca tccctcgttc ggaaagaaaa gcgcgcactt ccgcttccgt
     3541 tgcgtcctca acccatgcgc tacgatatgg cggcacggca cattcatcgc cgataaacaa
     3601 cgcattgtaa tcggcagaga cttgcgccat atcacagctt ttctgtaaac gcgtcagtaa
     3661 ctcatcctgc tccagtggcc agttcgcagc cagtttcccc tcacgaatca gggtaaacag
     3721 cggcaccagt aaaggatctt gcggttggcg gtaatagagc gaacccagca cacgacagag
     3781 gatagaaaac tcgttcatta atatatttcc attactagta attaaaggtc tgcaaattcc
     3841 gcaatcggtg ccataccgcg agattcaagg aagttcagta agcggcgcgg cgaaacattc
     3901 aaaatgcgct ctggcggaaa atctaccgcg tcgaggattt taagacactc ttcaaattcc
     3961 cccatggtaa acgcggtgtg agaatccgag cctaatgcca cccaacctcc agcatcacgt
     4021 accgctgcgg ctaccgcacg acagttgtct tcactgccct tacgtgagtg taaaaatgaa
     4081 gaattattga tttccagcgc cacctgatgt ttcgcggctg cttcagcaac ggctttcaca
     4141 tctatttcat atttgggatt tccgggatgg cttattatat gcacattgcc gctggcgata
     4201 gtggcgatca tcgcttgtgt attggtcgct ttgtcatgtg gtgcaaaaac cggctcatga
     4261 aaaccggcaa taatcagatc cagcgagtca aacattttac cgctgcaatc aatttcacca
     4321 tcaacatttt taatgttagc ttcgatgccg cgcaggatcc ctaccccatc aaccactcgc
     4381 ggccagatac gcatgttaat gaagtgccag tgatgcggag catcttccat atccgggcca
     4441 tgatcggtga tcgcaaaaag tttaatgccc ttttgtttgg cctgggcaat gtaatcactc
     4501 aatgtgctat atgcatgtgt gctggcaacg gtatgcatat gaaggtcgac gggatacata
     4561 actctctcct gtattcaatt cccgccaagg atagcaggaa tcctgatgct ttattagtag
     4621 ccgcgtgcgc ggtcgacttg cccgcagacc ctctcccctt tttcgagctg ggcaatggtg
     4681 cgagaaatgt actccacagc ttcagcggga cgggtaatcg cggcgacatg tggtgttatc
     4741 gtcacgcgtg gatgttgcca gagcggactt tcaggcggta agggttcacg attaaaaaca
     4801 tccaacattg cgcctttaac tttgccgcta tccagcgccg cgagcaggtc atcttccaca
     4861 acatgaacac cacgcgccag gttgaggaga tacgcgccat ccggtaattt ttcgagtaat
     4921 tgttgattaa taatgccgac ggtttcaggg gtattcggta acaaattaat caatacccga
     4981 cattggctca gaaatgcaga cagttcttcc cgtccggcaa agctttgcac gccaggccac
     5041 gatttacggg ttcgactcca gcaacgcagc ggaaagcgcc aggtttgcag actctgagca
     5101 actttactgc ccagtacgcc tgcgcccaaa atgccgatgg taaaatcttc ccgatgatat
     5161 tcaggcagcg gttgccaatg cgaactattt tgctggatgc gataatcgtc aaaacgtcga
     5221 aaccaatgca gcacctgact gacagcatat tcctgcattt gctcgcccat accggtatct
     5281 tccaggcgaa aaagtggaac agaagggttc agcatttcag ggtgtgcctg tagcttgctc
     5341 aaaatagaat caacatcggc cccgagtgcg aacaccgctt taagatcgcg ccctgccagc
     5401 atttcaacag gaggatgcca gactaaagca taatcagcag agtcattatc tccgcttttc
     5461 cacgctctga ctcttgcctg aggaatagct ttgcgcagtg cctcaatcca ccattgggta
     5521 tcgaacgttg ggtgataaaa gatgatatcc atactgactc ccgaaaagcg ttgtgcgaaa
     5581 tttattcgca cttatcgtta tgatctacaa aggccaccag cataacaaat ccgtggtcgg
     5641 tggcaaaaaa agcagatttc gcttattaaa accacacatt gattgaagtt tgaataaacg
     5701 cgcgattttt tcaaaaagtt tgttgacctc aggtcatgat ttccctaaat tagcgcccgt
     5761 tccagcaaga caggaacgac aatttggtga ggtgtccgag tggctgaagg agcacgcctg
     5821 gaaagtgtgt atacggcaac gtatcggggg ttcgaatccc cccctcaccg ccagattata
     5881 agcgcatgtt attgtttaac atgcgctttt ttattttccc ttccagacag tatgccatcg
     5941 ggtatgccat ctgaaaccgg tttatatgcc ccgcgatcat atcttatcgg acaatttccg
     6001 atctttgagc atactggcaa agctatactg ccagcatatc aggatagtgt tttgcgataa
     6061 tatcattcag tctgtcaacc agttctggtt ttctgaacgt gatatgcgcg gagcccttct
     6121 taaagtatct gatgctgaac atctcatctt catagctctc tttaccctga acagactgaa
     6181 tgtgatcatc cagacgaagg gtgatgtttc cccggttgtc ggggattggt ttaccgctga
     6241 acaggtgcaa cattctttcc agatcggcga gtcggtcagc ctgttgtccg gtgataagat
     6301 gaaatcccca ccggtcccat ctcaccagat tgttgacgat aattttactg ccaaatttac
     6361 aggggcaatt ggttttgtaa ttccagttca gccctctgaa gacgttgatc acccctcgct
     6421 caaacacctc atccttattc tggtgcagtt gttcaaatgt gctcagaatg ttcgcttcgc
     6481 tgatttccgg aaaattatcg tactccagtg accggtacca tgtatcacgg gcctgcgtat
     6541 ccattaatga cagcatcccg gaacgttgca tcaggtcacg ccagatttcg cgatcaagat
     6601 tccgggtaat ggctttcatt gctgtttcgg gtttctccat cagccagcag ccgcagcgaa
     6661 aatcctgttt catggcccag tccggggcag ttttacctcc gatgctttcc gtcagcaccg
     6721 agatttcagc cagttgtcgg ataagtgtgt cgatttgcaa cagtgtactc tttcgtacag
     6781 ccagaatgtg gcgaatgctg gttgagcaga taacgtccgt atgcccgttg accacttccg
     6841 gacgggagat tgtgatatct gacataatcg atattccttt ttgcagataa atgaaaacgc
     6901 cagccctgtg caggctggcg tcagaggaat gaagtctggt gagtgagctt caccggttct
     6961 ggtgcagaag ctgcagatga cgcagggtac ggacatcctc ccgggcctgt tcgatacagg
     7021 gcaacagtgc tgctgaatcc gttttatcct catcgttgtc aaagataatt cccgatccgc
     7081 agtcgatatt gtcctgcagc cacgcaatca gaatatccag cgctgtttcc gtggttaatg
     7141 atttcatgtt gttcctggtt accagtcgaa agtgggtaaa cccggcaggc attcagcgct
     7201 ggcatccaga tgaatgagac tgacgccata acgccggatg agtgtgacga ccagacgacg
     7261 gaacgttttt gacagcccca ggcgtttaag ccgcagaacc gggtatgacc acgcatcggt
     7321 acgtaacaga tatccgctac ctgtaaaatg aatccattct gattcaccga agtcactggt
     7381 ctggtgtgac agcgagtaca accaggcgtt gtccgtttcc gtgatatgtg cggtactgca
     7441 gcgtatgccg gtaagggtag caaatggtgg cgtgcattct gctgaggtct ggtcagattc
     7501 atcaacaaca cgaagggagt agccgttatc gcagaccatg ttaatcagtt cagcgagctc
     7561 aataccatcg acgtcaacga caatgcgccc catgttcagt gcctgtacgt taacgctgtc
     7621 ggcttccagt gtcagggcca gtttcatcat ttcgcctccg gatacttacc caaggtaatg
     7681 ttatttaccg ttctgtaatt gtcgcgggtc atcaggccgg tcgcccggcg agcccggagg
     7741 atatcaatac tgtttattaa ctgagagcgg ggacaggtgc tgaatcccgg ctggtcggta
     7801 cgcaccagcg cgtatttttc cacgagaaaa ttaactgcat cacacagtga aatgcctgcc
     7861 tcaatatgct gctcaatcac acgttcatcg gcaaacggtg tgtcattcag tgtgaggcca
     7921 taatgctggt ccagcagtcg gatgagcagt gtctgccaga tggtgacggg agacgggcag
     7981 cgagatacct cccgtacatg tgtgtcggat aatgttttca tcttaagttc tctgtaactg
     8041 gtagtgctaa aggtgagagc ttcggggtta tgaggtggtt gcgagtgctg gtgtcggata
     8101 aacagccaga taaacgtaac cacaggagcc gagggtgtcg gcttcgcagg tcagcccttt
     8161 tgcattcagc gtgacggtat gctgatatcg gggattcagt tcaccgctgg tgagcatgag
     8221 ttccagttgt ttcatcagca gcggaaaggc ctggtccaga tggtatgcat ctgcgtcgct
     8281 gaagcggcct ctgataccgg cgcggtcggc aaggtaatgc aggcggttac cctcttgcac
     8341 cagacgagcg ccaaaacagg gcctcactgt gcagggaagc ccccaccagg ggcggtcgtg
     8401 attgtcatcg ggatgagtta tcccggagag tttgtctgac acgataaaat cctctctgaa
     8461 aagtggttaa gaatggttta acgatgacag gtcagccggc gcgctgaccg gtttcggtga
     8521 agatgtatgc attgatgaga atggcctcat ccacagcggc gtctgaggtc atctggtcgt
     8581 actcgtttcc cgggtgacgg aacatcagtt cagggtgaca ctcatcagcc ggacatacgg
     8641 gccaaaactg tccttacggc gttctgcaaa cacggcaaga acgccgggaa tatcctgcac
     8701 ttcacgacca gtatacgctt cagcactgcc gtgccagcga tacttaccgg tacagaacgg
     8761 aaaaagacgg gatgtcggat gttgttggtg aatacgcatg gcttcaccac gggtgataat
     8821 tttcataatg ggatacctct gaagacagaa gataaaagtg aaaacaggtg tgatgtggtt
     8881 gtgaagggtt aaagcagacc atgttcggca aaggagaaaa cctggttgcc accgactatc
     8941 agatggtccg gcacccggat atccaccagg cccagtgcct gtaccagacg ttcggtgata
     9001 agccggtctg ccttactggg tgtgacttca ccggacgggt gattgtgcgc cagcaccacg
     9061 gcagcggcat tgtggtacag ggcgcgttta atcacttccc ggggatgaac ttccgtacgg
     9121 ttgatggtgc cggtgaagag ggtttcaccg gcaatcagct gattctggtt gttcagatac
     9181 agcacccgga actcttcccg ctccagtccc gccatgttca gaatcagcca ttcccgtgcc
     9241 gcacgggtgg aggtgaaggc cacgccgggt tcatgaagat ggcggtccag ggtttgcagg
     9301 gcccgcagaa tgagactgcg ctcgccgggc gtcatctctc cgggcagaaa ggaaagctgc
     9361 tgcattgtcc ctgcctccat tcagtcaatg atgcgcataa tggcgctgca ttccggatgc
     9421 tgcagggcgt aatcccgcag ccggtaatag tggaccgtca tggcataaca ctccgtacga
     9481 caggcatgat gactgtacgt catcagacag gcagcaatac cggcggcttc cgggctcatt
     9541 tcagcgcggt tgccgttcat ggcattgaac agtacccatg tttcgtcatc atcgtcatcc
     9601 ggttcgggtg ccataaatgc cccgccgttg ttcagggtgt acagattcca gataccaccg
     9661 cagtagtctt cgcaaagacg gtccatccag ccgaagacac ggggctccag ggtcacccac
     9721 tgtggaatga ggccaaaatg ctgcggccag aagctgatgc gctgttcatc agggacggga
     9781 gtggcaacca gctgaggtgg gttattccct gatgcagtgg ttacggaaac agaaggtgtg
     9841 gtggaattat gcgaaacggt tgtcatgagg ttattcctta taaaaagtaa atgaatggaa
     9901 gaagccccgg ggcaggaaca gatgtgagtc aggactgcac tttcagtgaa acggcatcag
     9961 cgcatgctct ccagcagcgt ttctgccatc acccacaatg cgcgattgag cttaatgtcg
    10021 gtatcaatgc tgtgaatggc ccgggtatgg atacgttttc cttttgcact gcgaccggaa
    10081 atcccgcctt tcagcatatt ctcctggatg gtctgataag cactccacag gtccttaccg
    10141 taatcctccc ggcgtcgtgg tgtcagaatg tcggcggtgg tgacgggctg atgttcgtca
    10201 ccataacggt aagtcagtgc cgcctgtgcc agcgcctggc gtgccggtgg cggcaggacc
    10261 agcgactgca tggcatcacg cttctcctct atccggtcaa aaacgcccac cacctcgtaa
    10321 gccccttcaa tgactctgtc caccacattt ccccggtgtg gaacacgcaa ttctcccaga
    10381 gactgaccgc agaccagtcc attggtacaa atggcacgaa aatatccggg taacatctgg
    10441 tagctggatg aaccgtcatg ggagttgagc agaataattt cagggacatg ctgaccggtt
    10501 atctgtccgg cccgccgcag acgcagcatg tgttttgtgt attcccggcg gctctggtcg
    10561 cgcacgcggg tctggcaggc aaagaacggc tgaaagcctt cctgctgcag attttccagg
    10621 acggtgatag tgggaatgta cgcatagtgt tcactgcgag aggtatgtcg gttttcccca
    10681 aaaatactcg gtacatggcg catcagttct tcgtgtgtca gcggacgatc acgacgtatc
    10741 tggttcgcat aaccaaaacg actggctaat ctcatgattt gctccttatg aatgattaag
    10801 cgatataaat gaaaaagccg cgtctcccgg agaagacgcg gcctgatggg tgaaatgaat
    10861 gaggtttatt gtctgagaag cccttcactg gcgaaccacg tattaagctg tattccggca
    10921 tcgccagtgc aactgacctt cagcattgcg gataacccgc cggaaatatg ttcactggtt
    10981 gtttttgtat cttcccccaa aattaaataa attcatctta accatcaccc agataatata
    11041 tatctgaaaa tattttcaat aacaccagac aggaaaaaca ccaccacagg cggtggcgtt
    11101 tctttggggc gtctttattt aatccgggcc atcgcgtttc tcagtgccaa atctgcctct
    11161 tccgttatat ccaggctttt ctgcaatacc gcctctgacc gggtcagtgc ctgacgtgct
    11221 gccagcggtt tccggatatg ctccatcgcc agcatcgtgt cttcatagcc ctgctgacgc
    11281 caggcgtcgg tatgtgccga tgtaaaactg agcagaccgc cgctgttgcc accatcacct
    11341 gcatatttca gccttttccg gggacggatc tcaaggattg tggtgtccgg aaaagcctgg
    11401 cgatcccata aagaaccgtc actcagatgc gtcacaatca ccatattgca tccggcatcg
    11461 accagaggcg tcacaggggt atttccctgc atatttcgcc atcctcccat accaccatca
    11521 ccgaacattg tcccctgaac ctcacgggga cggaacagca ggggcagcgc ggctgacgca
    11581 agcagagcct ctttctgctg tccgcggggc aggctctgga tatgctgaaa aacggcgttt
    11641 ttcgtggttc cgacacccag ttcagcacga atgcagtcaa taatatcctg catgccccct
    11701 tctgtggggt acagcgacac atacagcggt agcccgtctg ccagagcatc agtatcaaga
    11761 taatgatcca tcagcgctgt caggggctca tctgacaaca acggctgagc catcagattg
    11821 tcaaacccgt tgattgtcga tatatggcga agaagcgtcg taagcagtgc tcctgcacgt
    11881 ccggggatct ggcagaggtt cattgcctgg aacaattttt tcagcaatga aaagtaaacc
    11941 aatctgttta ccgacagcac ctgattattc cccagatgct cccagagcgc ctccaggcgt
    12001 acggcagctt ctgacagatc gggagaggcc gcgataatgg caccattgaa tgcgccaatg
    12061 ctggtccctg aaaccatgct gatctgtgta ccacactctg ccagtgcctt aaccactccc
    12121 gcctgataag cgcccaccgc cccgccaccg gacagcacca gccccgtttt catttctgta
    12181 ctcatgcagc ccccttctga aatatttcat cgaccatact ttcagagagg cgttctgcag
    12241 agcagaggcg aggcatgcga accggtaagt gttctgcagc ctgcgtggtt gaccagatca
    12301 ccggatgctg ctcttcactg caccagtctc ccagccagtt aatcgcgtca tgccactcgt
    12361 tatctgcctg ctcccggccc tgaccaccaa gccagtccag agtgttcaga cgcacggttg
    12421 cactaccgcc actctcctgt gccatctgcg ttaatgcttt atgtctgaga atatccagta
    12481 gctggttaac ctggcctgtt tcgccctgac gtatcacatc accaaacgcg ccccagcgaa
    12541 gctcttcaag tgccacataa caacgtccgg caggggaaaa tgacgcatat cgcccggcgc
    12601 tccaccatga gaatatctgc tcaagatgta actgcgcccg ctgaacctgg tcaacacggt
    12661 ggagtttttc ttcaagatga ttcagtttat ggtgaacctg atcggccagg gtgagtaact
    12721 gctcccgcgt atctgcggaa taatgcgcca gcctggctgt atcactgacc agtgaactga
    12781 ccctgtcgtt tacccgggta atcgcgtagt ttgtggtggc aagtgacgtt gtcatctccg
    12841 tcagccagcg cagggacgcc tcaacgccct gtgcaagcga cgcattgatg gcattctgcc
    12901 gtgcagcgcc ttcgccggtc agcccctctt ttaaacgccg gaagaacgtg cgctctccgg
    12961 ccgtgcggat atgctcctgt gcaacatcga ttccgtttgc gaaatcaacc acaaattttt
    13021 caggaagaca ggtgagtacc tgacataccc ggttatcagc aatttgttga ataaatggtg
    13081 aagtcacagc attgtctcca gctcttccct cagcgcctta ctgtcagtaa tgagctcagg
    13141 aaccctttca tttaatgcct gaagtcgccc ggtaagccgt tcctgcgcct gctgatcgcg
    13201 tttatgatct tcagagctct gaatcagcgt gttacgcagt tgttcaactt tctccctgaa
    13261 tgcgcagaaa aaggcatcga tttcctggcg gacgggctgg ttaatatcct gctcaatgga
    13321 tgcctgcagc tccccgaaat atgcccgggt cagtgacata acttcctctc tggccttgtt
    13381 gatattgatc acgctgcgac tcacatcctc gtcatagttt tcccagccca ggtcactggt
    13441 gccaaaccat cgacaaacgg ttccccatac gccactctgc cgacggcgac gggtgacggt
    13501 ctccgttctt tcctccataa gcgcactcag atttattttt accgcgagcc gggtctgtaa
    13561 ctggctgact gccggaaaac tgatttttac ggtaaagccc tcatcctcca ggcgggcatt
    13621 gatgcgaccg gcaatgtttt ctgtggcgta cagggccgtg ccacgaaaac ctgtttcaat
    13681 cccgctgacg attttctcca gctcaggccg gaactgtgct tcagcctcac ggtgcaggct
    13741 ggtcacggtc gattcgattt gcgtcatcag ttcaagggct gccctgcgat cactgaattt
    13801 tatttcgggg ctgtccggat caaagtcccg catcctgtca tgctgatttc cggttccgaa
    13861 aatatcgtga aagaaagcga atgcgtttct ctcccggggc tgcgctgaat tttcttcctc
    13921 cagcatttgc tgttctttgc gtttaccgct tctgaaatag tcgttcagct cctcctccag
    13981 tgaggcacag accttcgtca gtaattcccc ggttttggta ttgatgtcct ccatggccat
    14041 tctgacatca ccggtcacct gattctgaca ttcctcaatc tcctggatat ccgccagcag
    14101 gctggtaata tgcgcctgta gtgactgaat gctttgctgc agcccctgat gacgtaatga
    14161 cagatattcg ctgacatttt ctgcattctg catcagtttt gcggcggcag agtcaaccgc
    14221 cagtgccgcc gctctggaat gggagctctg tatgacttcc gttatcagtt gatcgatcag
    14281 agatatattc cagagttttt ctgccttttt atttacctta gtgctgtctt tccagtcgtc
    14341 ctcttccacc ataggtccga aagcttccct gataaaatca tcgacccagg cttcatcaac
    14401 cggaagcgca ccgttttttc gtaactcatg aagcgcacgg ttagccaggt acgcctgacg
    14461 tgatgagccg ggataaacgc gggaggcggg cagcacatcg ctgttcagca ttgctggcac
    14521 cttctggcgt acggcatccg caccatcacc attgcgatct ttttcatcga atttattgac
    14581 cagcacaaac agacgaccgg ctgagacgtc agcaatggca ttaagctctt tacgaacctc
    14641 ctcatcggct ttcgagttca tctgggtgta atccatcacc gccagcaccg cagaggcttt
    14701 ctgtagttga tcccgcatca tcatttccat ctgtggttgt ccggcttcat tagggccggg
    14761 ggtatccagc agtgtgagtg ttccctgaca tgcatccatt cccaccaggt ggctgaacgc
    14821 cacgtcgatc accggtagtt tctgcacttc tgcgtattcg tcaaaaggaa attcagtccc
    14881 cattgccgca gcaagacgaa ccaggtcatt taacgatgcc agtccggtaa agatttcgtc
    14941 ttccccgtgg tattcatttt taagccagac atcatccgcc agaatatcca gtagttcgcg
    15001 ggtatccccg gtttgctgca gggactgtgc gacttgctgc cctgccgggg tggcgagttt
    15061 ttcctgcagt gtgattaata aattgcggac gggctgaata tgttccagat ggaggaccgg
    15121 ttccgttttt ccgggaacgt ggcggatcaa tgtcggtacc gaggtcatgg ggcggttacg
    15181 gttgggcaga atttcctgcc ccacaatggc gttgatggtg gttgatttgc ccgccttcat
    15241 cgtgccgacc acagccagca ccatctccct gcgggcggtt ttgatttgtt cgccctccag
    15301 ctcctcaatt cttttcagcg ctttccgttt atcaaaaagc agcccgtttt cgttcttact
    15361 gtcagataac acatccggct catcaaccat ttgccggagc agattgatat tcagttgcaa
    15421 aagccggtcg gcttcatcac aaagcagggc gatgttcttt tcgtgcatca ttcaccttta
    15481 taaattcaat aattcagaac tgcggtgaac agcaatgagc gtcctttccc attactgcat
    15541 gaattttcct gaaatcgtat ttccgggata ccgtataatc agaaagtcat attcagcgta
    15601 gcctgaccgt tatagccttc agcgctgctg ccgctgacgc tgtgggcata accggcctga
    15661 acgcccaggg tgatattttc ccggatacgg gcttccagtc cggcctgcag gtccagtgac
    15721 gtgccattcc gggacggtga gaacgtcatg ttactgccgg ctgcggctgt ccccatgctc
    15781 atgtcacccc gggagctgaa ggtgcggata acagaaggct gtacccacca gttcaccggc
    15841 agttcactca cactgtgttt tgcactgtcg cgcagggtgt cacgggatga ggtgccttca
    15901 ccaaaggtca tatcgttgtg gctgcccaga cggaaaccgg cacgcacatg ttgtgcactg
    15961 ccatgcccga acttcacata accggcgtta tcctggccgt catccaggga gagtccctgc
    16021 caggtgtact gcagttgtgg ctccagcatc agattgtcag tgatactgaa gggcagaccg
    16081 gtttccagtg agcccagcca gccccagccc cgggcgcgga agtcgttatt gtccgatgac
    16141 gctttcatgc tgtgacgggt tccctgggcc acaatgtcag cccacaggcc ggaggatgtg
    16201 tgtaccagat tcaggtatcc gcccagactg ccggcatcat cccggaccgt gccggcgcgg
    16261 gaaccgtcat catccttaac atcaacggaa gaatggcctg cagcaccata cacccctgtc
    16321 gtcagagaca taccggcaac ctctgttctg agcaggtcac cctccagacg gacgaagcca
    16381 tagctgccgc tgctttccgg cgtggctcca cgggcaatac cgccgttgtt atcgtgaccg
    16441 agatgaccgc cctgaatgct gagacggacg ctgttatttt caccgtttac accggtctga
    16501 tggctgcggg agcctgccag aatccggtca tagtccattg cctgtgtcaa catggatgta
    16561 tacaggggga cttcagcacg ataagcattt tcactgcgca ggtaccagtc ttcatcgctg
    16621 tcacggttca gggtgtagtt aaaggcgccg gcctgaagcg ggcgactcag ggcaaacgca
    16681 ccttcttctg tggtggcgcc attctgtgca tccacaaccc ggataccctg tccggtggtt
    16741 gccaccccga ggttgctgtt tccgacattt gtaaacgcaa gccaggtttt gccggttgcc
    16801 tgaccaccat taatcaccag ctggtcagag gcattgctgc catcaaggcg aacacgcata
    16861 ttgatggtgc cgccctggcc ggtgaggttc gtggtggtca gtttatggaa cgttaccggg
    16921 gagttacttt ttgcaacagc acggctcagc gcggcattcg gtgtcgtctg attatcaaag
    16981 cgaatctccc ctgcgttaac aatatcgccg gtactgacca catcaccatt cagtaccata
    17041 ctggcgcctt tactgagctg tgttctgcca ctcagacttc ccccggcagc cagcgtcagt
    17101 gttccacgat gtccgacagt ggtgttgcct gcctgtcccc cggcattaac cgtaaagctg
    17161 ccgccatttt ccagcagcag gccgctggcc tgaccggatg tgccatcaat actgaactta
    17221 ccgctggcat tggtcccctc aacagtggca ccactgtctg caatcagggc accaccggat
    17281 gttatggtga cacttgtcgc cttaccgccg gcagacactg ccagggtacc gccgtcatcc
    17341 actagggtat tctgtgctga atggctctcc agtacatcca gacgaccgcc ggattccaga
    17401 acaacaccgt cagccttacc gttttccacc gtgaaattcc ccagacggtt tgtgccgatg
    17461 acagttgcag cagtactggt aaccagtgca ccgcccgtgt tctgggtgac tgcagtggct
    17521 tccccgccgg catgtaccct cagttcaccg ttctgattta tggtggtgtt accggcagcg
    17581 ccacctgcct taacaacctg ccagcccccc tcgttaatta ccgtgttcag cgcaagtccg
    17641 tccctgtgca catactggta accgccattc agtgtggtat tcagggccct tccgtgtacc
    17701 gactggtcac cacctgcgta aatgagagtg tcacgggcta gcccggaaaa taagataatc
    17761 tgccgtccat ttttgttgat ggtggtggat tctgctgttc cctggacctt ctgacccgta
    17821 tacgagttgt cagaatcagg gccgccttct gcgccggtgt tgatgatggt tcctgttgcc
    17881 agcccgccac ttttaacgct ctggtaaccg tcgcggttga taattgtacc ggtggcaacc
    17941 ccgccctcat gcacccactg ctcgcctctg ttattcagtg tggtgttcac cgccagtccg
    18001 ttcaggctct gtcccccgcc gtcacgaata accgtatcac tggctgttcc cccctccagc
    18061 acgacctgac gaccatttgt ggtgacagtg gtgtttccgg ctatcccgcc attctgtatc
    18121 cattgcccac cggtgttttc ttcactgtcc ggccccagtt ccagcccggt actgatggtc
    18181 attccgttgg ccgtaccgaa gacaatctgg ttgtcatgat ttgtcagtgt tccatcgttc
    18241 acggtttctc ccgcctgtac aaccttgtca gcagccagtg ccgggactga tgtgacagca
    18301 gcaagagaca gcgcaaccgc cacaccggcg cgttttcccc gtgagcgggc cagttcggag
    18361 gccaccacca gggtgcccgt aatgtgattc cataccagcc tgtagctggt gttcagatgt
    18421 cgtttcatca gcttttcctt acagagggtg aataaaaaag ccggtaccca caaaagtgga
    18481 taccggcagg tacaaaacag agttatcggg cacagcgata cgtcccgggc gtaaacgctc
    18541 tcccccttca tcagacagag aagtatcagt ctgttgtgtg caattccgtt ctgcatgatg
    18601 aagagattca gctgcctttt cgcaggccat taaccactat aaacgatcga tataaacgat
    18661 cgatacctgt tttatcgatc gttttattct attaacagta gagcagtcac gcaagagaaa
    18721 caggaggggc atgaggtgtt tgtatcatgt tttcagttgg ttaacgcaat gtaatcagac
    18781 atttcccctg gcagactgtt cagaagaaaa tccagttcca taccgcgcgg gcaacagaga
    18841 ccaccgtgtc acggacggca cgcagaactg tgcgtgcaac agaggcaaca cagacgctct
    18901 ccaccgtgtc aaatatccgg tccaccgcac cggtaaactg ttcacgggcc tgcccccgga
    18961 cagattccgt gcacagctcg tcctgcaggc gggtcatcag gggactggcg gcatggtcgg
    19021 gaagcgctgt catgagcgca ctgaccagcg tatccagttc ccagccggtg cgggccgata
    19081 cggcaacaac cggatgtacg ggccggaaca gccggaatac cgcatctgtt ttttcgcgaa
    19141 tgttctgttc ctgtgcggga gaaggctgaa tacctgccat atcccattca tggcagggct
    19201 ctgttttgtc ggcctgcgtc accacaaaca gcacctgctg atgtccccgg tgcagtatgt
    19261 gtcgccagaa atactcatcc acagacaggg cacggtcatc ggctttaatc agccacagta
    19321 ccaagtccag ttcaggcaga atgtcacggt acagggcttc atactctgca tccctgtccc
    19381 ggctctcgcc caccccgggc aggtcagtga taaccatgct gtgaccatgg ccactcagac
    19441 ggaagcgccg cacttcccgg gtgccggcgt gaacatcact gaccggggtg acctccccct
    19501 gaaacagtgc attacagagt gaggatttac cggccccgct tttacccata atgccaatca
    19561 cgggttcgtg actggtgagt ttgcgcagat gt
//
LOCUS       DAZGOB010000087        19592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGOB010000087 DAZGOB010000000
VERSION     DAZGOB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.087"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            72..974
                     /locus_tag="ACHLIF_003318"
     CDS             72..974
                     /locus_tag="ACHLIF_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9607280.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            1121..2341
                     /locus_tag="ACHLIF_003319"
     CDS             1121..2341
                     /locus_tag="ACHLIF_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO9607281.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2314..2943
                     /locus_tag="ACHLIF_003320"
     CDS             2314..2943
                     /locus_tag="ACHLIF_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO9607282.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2944..4104)
                     /gene="ybdL"
                     /locus_tag="ACHLIF_003321"
     CDS             complement(2944..4104)
                     /gene="ybdL"
                     /locus_tag="ACHLIF_003321"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO9607283.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4213..5301
                     /gene="hcxA"
                     /locus_tag="ACHLIF_003322"
     CDS             4213..5301
                     /gene="hcxA"
                     /locus_tag="ACHLIF_003322"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO9607284.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5311..5508)
                     /locus_tag="ACHLIF_003323"
     CDS             complement(5311..5508)
                     /locus_tag="ACHLIF_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO9607285.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5690..7795)
                     /gene="cstA"
                     /locus_tag="ACHLIF_003324"
     CDS             complement(5690..7795)
                     /gene="cstA"
                     /locus_tag="ACHLIF_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO9607286.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7976..8389)
                     /gene="entH"
                     /locus_tag="ACHLIF_003325"
     CDS             complement(7976..8389)
                     /gene="entH"
                     /locus_tag="ACHLIF_003325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO9607287.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8392..9138)
                     /gene="entA"
                     /locus_tag="ACHLIF_003326"
     CDS             complement(8392..9138)
                     /gene="entA"
                     /locus_tag="ACHLIF_003326"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO9607288.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9138..9995)
                     /gene="entB"
                     /locus_tag="ACHLIF_003327"
     CDS             complement(9138..9995)
                     /gene="entB"
                     /locus_tag="ACHLIF_003327"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO9607289.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(10009..11619)
                     /gene="entE"
                     /locus_tag="ACHLIF_003328"
     CDS             complement(10009..11619)
                     /gene="entE"
                     /locus_tag="ACHLIF_003328"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO9607290.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11629..12804)
                     /gene="entC"
                     /locus_tag="ACHLIF_003329"
     CDS             complement(11629..12804)
                     /gene="entC"
                     /locus_tag="ACHLIF_003329"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO9607291.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            13173..14129
                     /gene="fepB"
                     /locus_tag="ACHLIF_003330"
     CDS             13173..14129
                     /gene="fepB"
                     /locus_tag="ACHLIF_003330"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO9607292.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(14133..15383)
                     /gene="entS"
                     /locus_tag="ACHLIF_003331"
     CDS             complement(14133..15383)
                     /gene="entS"
                     /locus_tag="ACHLIF_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO9607293.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15494..16498
                     /gene="fepD"
                     /locus_tag="ACHLIF_003332"
     CDS             15494..16498
                     /gene="fepD"
                     /locus_tag="ACHLIF_003332"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO9607294.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16495..17487
                     /gene="fepG"
                     /locus_tag="ACHLIF_003333"
     CDS             16495..17487
                     /gene="fepG"
                     /locus_tag="ACHLIF_003333"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO9607295.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17484..18299
                     /gene="fepC"
                     /locus_tag="ACHLIF_003334"
     CDS             17484..18299
                     /gene="fepC"
                     /locus_tag="ACHLIF_003334"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO9607296.1"
                     /translation="MNESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18296..19429)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHLIF_003335"
     CDS             complement(18296..19429)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHLIF_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO9607297.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4716 a         5465 c         4959 g         4452 t
ORIGIN      
        1 taaaaatata tacgcaaaaa acattgatta agtgaatata ccatggaaga aaaatataac
       61 cggagtagtg tatggccaat ctctacgact tgaaaaagtt cgacttaaac cttcttgtca
      121 tatttgagtg tatttaccag catttaagca ttagcaaagc tgctgaatca ctatatatca
      181 ccccatcagc cgtgagccag tcgttacaac gcttacgtgc gcaatttaat gaccctttat
      241 tcattcgctc aggaaaaggt atcgcgccga cgacaacagg gttgaatctg catcaccatc
      301 ttgagaaaaa cctcaaaggc cttgaacaaa caattaatat agtcaataag tccgaactaa
      361 agaaaaactt tattatttac ggccctcagc tcatttcttg ctctaataat agtatgctga
      421 tccgctgcct acggcaagat gcctctgtcg agattgagtg ccatgatatc ctcatgtcag
      481 cagagaatgc tgaagaactg ttggttcacc gcaaagccga tctggtgatc acccaaatgc
      541 cagtgatcag ccgttccgtc atttgcatgc cattgcatac aattcgcaat actctaattt
      601 gtagtaataa gcatccgcga attactgaca atagcactta tgaacaaatt atggcagaag
      661 agttcaccca attaatatcc aaatcagcag gagttgatga tattcaaatg gaaatagacg
      721 agaagtttat gaatcgcaaa atttcatttc gtggttcctc tttgctgaca atcattaata
      781 gcattgcagt tacagattta ctgggaatag tcccctatga gctttataat tcctaccgtg
      841 actttcttaa tttaaaagag ataaaactag aacatccgtt accttcaata aagctttaca
      901 tttcctataa taaatcttct ttaaataatt tggtgttctc ccgatttatt gaccgtctaa
      961 atgagagttt ttgatatata actacagaca gctactataa ctttcatcta tttattcaca
     1021 gcgcgaatga aaaataaaac actctgcttt gcagtgttta ctttataatt aacagactta
     1081 tctcactgat caccctgtaa cgttcagaga gcgtcttcag atgtctattt ataaaattcc
     1141 tcttccgctc aatattctgg aagctgcacg agagcgtatt acctggacac tcaacaccct
     1201 accccgcgtt tgtgtctcat tttctggcgg caaagactct ggtttaatgc tgcatctgac
     1261 agccgaactt gcccgacaaa tgggcaaaaa aatctgcgtt ttgtttatcg actgggaggc
     1321 gcaattctct tgcaccatta actatgttca gtccctgcgc gagttgtaca ccgatgtcat
     1381 cgaagagttt tactgggttg cgctcccgct tacgacgcaa aattcccttt cacaatacca
     1441 acccgaatgg cagtgctggg aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc
     1501 gataaccgat cctgactttt tctgctttta ccagccaggc atgaccttcg aacaatttgt
     1561 acgtgagttt gccgaatggt tttcacaaaa acgtccggcg gcgatgatga tcggcatccg
     1621 tgcggatgag tcctacaacc gttttgtcgc catcgccagt ttaaataaac aacgttttgc
     1681 cgacgataaa ccatggacta cggccgcacc aggcggtcat agctggtaca tttaccccat
     1741 ttacgactgg aaagtggctg atatctggac ctggtatgca aatcatcaaa atctctgcaa
     1801 cccactgtat aacctaatgt atcaggcagg cgttcctctg cgtcatatgc gaatttgcga
     1861 accttttggc ccggagcaac gacaaggatt atggctctat cacgttatcg aaccggatcg
     1921 ctgggctgct atgtgcgcac gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca
     1981 tgataatcat ttctatgggc atcggaaaat cctcaagcca gaacatttag actggcaaga
     2041 atatgcatta ttgctgctca atagcatgcc ggaaaaaaca gctgagcatt accgcaataa
     2101 aatcgccatt tatttgcact ggtatcagaa aaaaggcatc gaggttccac aaacccagca
     2161 aggggacatt ggcgcgaaag atatcccctc ctggcggcgg atatgcaaag ttttactcaa
     2221 taacgattac tggtgtcggg cattatcatt tagtcctacg aaagcgaaga actatcagcg
     2281 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
     2341 acgcaggatt taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
     2401 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
     2461 gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca
     2521 ccacccgaaa aaaaactgct gaaaaaatcc attgaaatag atggttttac ccaacccatt
     2581 gtggtcacgc atacggataa aaatgccctg gaaatcgtgg atggttttca ccgccatgaa
     2641 attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt
     2701 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga
     2761 cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac
     2821 gataataaaa ttggcaaaga gctaggaatg gacagcgacg aagtgctgcg cctgaagcaa
     2881 attaacggcc tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa
     2941 taactaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
     3001 acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc
     3061 cgctacgccg tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagaaac
     3121 ctcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct
     3181 ttcatttaaa gcattcacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag
     3241 ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac
     3301 cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac
     3361 acaataaccc actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac
     3421 tgcccgctca cgcagctgcg gatgcgccag cacactggca tggccctgtt gtgaaaagtt
     3481 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     3541 caaagcggcg aaatcagcct gctgccagac agttgcactg gggttatgcg gagtgttgag
     3601 gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa
     3661 atgcggtggt tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcggggga
     3721 gtaactgtca tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt
     3781 aatcgccgcg tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     3841 tggttgatag ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     3901 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     3961 taaatagcgc ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg
     4021 ctgcgccagc gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     4081 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     4141 ctataacacg atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa
     4201 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     4261 tcacatccag gaagtttcaa tcacctgcac gattttttca ctgatgaaca actttctcgc
     4321 gcggtgtgga tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg
     4381 tttggacttc caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta
     4441 cagcaactgg cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     4501 gcactgctcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccgtt tgttgccgtt
     4561 ccgacgatcg ccgccacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     4621 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaaccg
     4681 gagattatcc tcaatgcacc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg
     4741 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga
     4801 ctggggatca ataatgcgca ggccattcgc gacgtattgt taaacagtag cgaacaggcg
     4861 ctgagcgatc agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     4921 attgctggtg gtgggatggt cggtggtctg ggcgatcgtt ttacgcgtgt ggcggcggct
     4981 catgccgtgc ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc
     5041 aaagtcgctt atggcattct ggtacaaagc gccttgctgg gtcaggatga tgtgctggcg
     5101 caattaaccg gggcgtatca gcgttttcat ctgccgacca cgctggcgga gctggaagtg
     5161 gatatcaata atcatgcgga gatcgacaaa gtgattaccc acaccctgcg tccggtggaa
     5221 tccattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa
     5281 gtggaatcgt ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc
     5341 gtagcgcgcg tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg
     5401 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt
     5461 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc
     5521 taaccatgtt gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg
     5581 ctatcaacgc gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggagga
     5641 accctcggca ctatgccaac attcgccaac atcccccctc actctgactt tagtgtgcgc
     5701 cttttgcctg tgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt
     5761 tcgccgttgg cttcggatct ttcaatgccg ccagtgccgt cttaatcgag aacagcgcca
     5821 gaaccaccac gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca
     5881 ccagttgcgc cagctgtgac tcagtatact gcgacggaat attgccgctg tcgatcattg
     5941 cctggaactt attagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct
     6001 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     6061 cgtaacgttg acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg
     6121 ccagcatctg gttggcaata ccaaacagcg gccacagagt gttaatgccg cccagcggat
     6181 cgaccacgcc ctgatggagg aagtagcccc acgccagtac gcacagcgct gttgccagca
     6241 ggttagcagg cagtgaatcg gtccgcttca ggcccggaga aaccacgccc agcagatcct
     6301 gcaacataaa gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt
     6361 caaacagaat ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt
     6421 gcaggatgta agccatcccc accgccagcg tcggcgcacc gcccgcacgg gaaatgatcg
     6481 actgttcacc cacttcgctg gcaatctggt tgagcgtatc tggtgtaatg ctaaagcccc
     6541 agctactcac cacctgcgcg gcagaagcga ctacatccgc cgtccctgcc ggagccagca
     6601 ccgccattgg gctgttcatg gcaaaataca cgcccggatc gatgatacag gcagaaacca
     6661 gcgccataat cgccacgaag gattccatca acatcccacc gtagccgata aagcacgcct
     6721 gcccttcgtt cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagccag
     6781 acaccgcacc acaggcgatg gtgataaaca ggaacgggaa caggttaccg gtccataccg
     6841 ggccagtgcc atcgacaaat ttggtcagcg caggcatggt cagcgtcggg cgcataatca
     6901 aaatgcctac cgccagaccg acgatagtcc cgattttcag gaaggtcgag agatagtcac
     6961 gcggggccag cagcaaccac accggcagca ccgccgccac aaaaccgtag cccaccagca
     7021 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcgga ctttctgcca
     7081 cccagccgcc agagataatg gcgaaaatca ggaacaccag gccaatcact gacacttcgc
     7141 caatgcggcc cggacgcaga tagcgcaggt agattcccat aaaaatcgcc agcggaatgg
     7201 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     7261 ccagtactgc aaggataatg accatgatca taaagcaggc taccagcgca atcaccccgg
     7321 cagttggccc catctcttct ttgaccagct cacccagcga tcgaccgtca cggcgcgtag
     7381 aaacaaacag caccatgaaa tcctgcaccg caccagcgag aaccaccccg gcgagcagcc
     7441 agatcatccc tggcaggtaa cccatttgcg ccgccagtac cggcccaacc aacggacctg
     7501 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt
     7561 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     7621 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     7681 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     7741 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     7801 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtaagat
     7861 ttaacaacac ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     7921 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     7981 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga
     8041 cgatttccca gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct
     8101 tgccttcaga caccgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc
     8161 cgtcacgggt catcataaat ccggccatcg atcccagcgt ttcagccagc gccgccgacg
     8221 cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt
     8281 ccagcacatc atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat
     8341 cgctggtggc gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     8401 cagcgttgag ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     8461 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     8521 gccgagttta aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac
     8581 ccacagcgtg cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac
     8641 gccgctaccc gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga
     8701 tgcgccataa gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac
     8761 aatcgccccg ccccgctgac ggcgaaactg gttcatggtt tgctggaaca ggttaaacgc
     8821 accgccgacg ttaaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc
     8881 gcccatgcgc aaaattcccg ccgcattgat cagcacgtcc agtcgctccg tttcagctaa
     8941 cagtcgctga catacctgcg cgacctgcgc agcatcggca acatccatca cttcggtcgc
     9001 aaagggatat tgctcctgag cgaacgcttg atcaaaaccg gtaactttcg ctcccgcctc
     9061 aacaaacgcc agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt
     9121 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     9181 gtcgggtttt tcgccagcat gacaaagtcg atatcaccat gcactttgcg ccagcgcgcc
     9241 gccagcgcca tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     9301 ggttcatcgg actcgtccag caacggcagg ataacctcac gcagcgccgc tttgctggcg
     9361 gggacaggtg ctggcagtaa ttcttcagtc atcaccaccc ggccagaccg cccggccaca
     9421 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata
     9481 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     9541 tataccccgg taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga
     9601 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     9661 aggcgatcca ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca
     9721 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccgga
     9781 atattgtgct gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc
     9841 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     9901 gcggcacgtt gcggttcaaa ggcccagtca actttattct tagggatatc gtgagactcc
     9961 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
    10021 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
    10081 aagtgaatct acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
    10141 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcgcagct
    10201 tttttcgccc atcagctcat cttccatgct caccagtgcg gcgtagatca ccgccgggtg
    10261 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcaccgccac ggttgatctg
    10321 atctttctcg cgcccctgca cggtgatgta cccctctgga tcaatagaga tcagatcgcc
    10381 ggaacagtaa aaaccgttgg catcaaaggc gctggcattg tgctgcgggc ttttgtaata
    10441 gccacggaag gtgtacggac cgcgggtcat caggcgtcca acttcccctt gcggcagtgg
    10501 atttccttcg gcatcggcaa cccacacttc atcgtccgga cacattgggt aaccctgggt
    10561 atggataatt ttctccgcgc tatcatcaag acgggtgtag ttcaccagcc cttccgccat
    10621 gccaaacacc tgctgcaact ggcagccaat ctcagcggga atacgcgccg caagggtggc
    10681 agaaagacgt gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
    10741 accttcggtc agcgcctgca accacaggct gactgccggt ggcaccagcg cggtgacgtt
    10801 cacctgatgt ttttcaatca atgggaagca aagcgtggcg ctgggatcgg ccgccagaac
    10861 aaccgttcct ccggcaagaa agacgcccag cgatcccggc gaactcatgg cgtagttatg
    10921 agccgccggg atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct
    10981 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagtttcg gtgtgccggt
    11041 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggtgatggcg tggcagtaaa
    11101 atcctcagcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgtgcag
    11161 ttgcaccacg cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct
    11221 aaacagcgca tgttggcgat cggcaatcag caatgcgggt tcaatctggc tggcataggc
    11281 gttcagttca ctacgctgat ggctgaacag cgccagcacc ggcgcaacgc ccagtttcag
    11341 cagcgcgaaa aaggtaatat acaattcagc gacgttaccc agttgtacca gcgcggtttc
    11401 accaggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag
    11461 ctcccggtaa ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
    11521 gcgagtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg
    11581 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
    11641 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
    11701 cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
    11761 gcagcggatg gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc
    11821 gccaaacagt tcgcggtcga atggctccag ttcagcaata acctgggtcg cggcctgatg
    11881 cgggaaaccg ctcagcgcgg gggttggatg cagcagacag gccagagtca gtgcgttttc
    11941 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
    12001 caattgtgga gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat
    12061 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
    12121 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
    12181 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
    12241 accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    12301 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac
    12361 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    12421 cgtggtttgt tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtaaa
    12481 acggcgggct gaggtctgtt tttcctggcg ggagaacgac tgccaggatt cgggaatata
    12541 cagcgacgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    12601 gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    12661 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    12721 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    12781 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    12841 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    12901 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    12961 cattattgat ggattcgcat aagcgcaatg tgatgtcctg cgccgttctg ccccctctcc
    13021 cttccagggt gagggctggg gtgagggtta atgttcgcac cagtgctggc tgttcccctc
    13081 accctctccc caaaggggcg aggggacgga ttgtgcgctt tgtcgaattt gtcattacgc
    13141 ccataacctt attaataaca ggaagctgat ttgtgagact cgccccgctc taccgcaacg
    13201 cccttctatt aacaggactt ttgctttcag gaatagccgc agttcaggcc gctgactggc
    13261 cgcgtcagat tactgacagc cgtggcacac atacactgga aagccagccg cagcgtattg
    13321 tttccaccag cgtcaccctg accggctcac tgctggcgat tgatgctccg gtgatcgcca
    13381 gcggcgcgac cacgccgaat aaccgcgtcg cggatgacca gggcttttta cgccagtgga
    13441 gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg agcaccgaag
    13501 ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg gattcggcgc
    13561 tggcactgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat tacgacgaca
    13621 aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag aaacaagcgg
    13681 cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa atcaaattac
    13741 cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc aatctctgga
    13801 cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg gcgaagttgc
    13861 ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt cagcttggtg
    13921 gggaaaatct ggctgctggg ttaaatggcg agtcactatt cctgttcgcc ggtgatcaga
    13981 aagatgccga tgcgatttat gccaatccac tgctcgcgca ccttcccgca gtacaaaaca
    14041 agcaggttta tgcgctggga accgagacgt tccgtctgga ttactacagc gccacgcaag
    14101 tgctggaacg acttaaggcg ctgttttaag cattaactgt cagacgctgt cacctgcggc
    14161 ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac gccgataatc
    14221 aacaaaccaa aaccgcttgc gcttgcggaa gcaaccggtg tcatcatcgc gcccaaacca
    14281 cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca caaaccgtta
    14341 atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg cagcaacgaa
    14401 ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat ccacatcggc
    14461 atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag catcaataac
    14521 cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc cgcgccgagc
    14581 gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt gtcagccagc
    14641 gccggataca gtacccgcac cgcgctcgcc atcgtcaata aaccacccag cagcgcaatc
    14701 ccgcccacca gcgggctggc aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga
    14761 tgctcacgcg gctgcggtgg cggtggcaac gccggaaggc ttaacaacgg tagcaaggta
    14821 ataaacgtgc ccgccgccgc cagcccgtag ttccaggcta cgccaccggt cgccagcaat
    14881 aaaccgccaa tcatgggcga aatcaccgac cccagacgca cggtcaacat ggtgatcgcc
    14941 ccggcctgca ttaagttttc acgccctacc agtgctggtg tcgccgccaa tagcgccgta
    15001 acgccaagcg atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac
    15061 ggctccggca gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc
    15121 gccagcaaaa tcactttttt gcgctcatag cgatccgcca gcacaccgcc aaccatcagg
    15181 ccaacaaaca tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgc
    15241 gtcatcatct ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag
    15301 ataaaacgag cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta
    15361 agcagccagg attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt
    15421 cttaggctgc acatgctaac atatccaaat aagatagata acgataatta atttcattat
    15481 catggaagtt cgtatgtctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt
    15541 gctgttatta ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc
    15601 cgcttccgta gtgctggagg ccctctccgg cacctgccag agcgccgact gcaccatcgt
    15661 gctcgacgcg cgactgccgc gcacccttgc cggtttactt gcaggcggcg cgcttggcct
    15721 tgccggggcg ttgatgcaaa ccctcacccg aaatccactt gccgaccccg gcttgcttgg
    15781 cgtgaatgcc ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc
    15841 cgcgcaggaa caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc
    15901 ctttaccggc agtcagggcg gcgggcagtt aagtccggtg cgtttaaccc tggcgggcgt
    15961 ggcgctggcg gcggtgctgg aagggctgac cagcggcatc gccctgctta atcctgatgt
    16021 ctacgatcaa ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt
    16081 aaaagtggtg ctgatcccgg tgctgatcgc cggagcaacc gcgctattac tgagccgcgc
    16141 gctgaacagt ttaagcctcg gcagcgacac cgcgacggcg ctgggcagtc gcgtggcgcg
    16201 cacacagtta attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcggtagt
    16261 tggcccgatt gcctttatcg gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc
    16321 cgatcatcgc tggtcgctgc ccgtcacgct acttgctacc cctgccctgc tgctgtttgc
    16381 cgatatcatc gggcgggtga ttgtgcccgg cgaactgcgc gtttctgtgg tcagtgcgtt
    16441 tattggtgca ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gcgcatgatt
    16501 tacgtctctc gccgattaat catcacctgt ttgctgttgg tttccgcctg tgtggttgca
    16561 ggtatctggg gattacgcag cggtgccgtc acgctggaaa cctcgcaggt attcgccgcg
    16621 ctgatgggcg atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc
    16681 gtgctgatgg cgctgttgat tggcgcggca ctgggcgtca gcggcgcgat ttttcagtcg
    16741 ctgatgcgta acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc
    16801 ggcgtgctgg tagcgatggt gctgtttggt caggacctga cggctatcgc gctggcagca
    16861 atggtgggcg gcattatcac ttcgctgttg gtctggctgc tcgcctggcg taacggcatc
    16921 gacacctttc ggttgattat tatcggtatc ggcgttcgtg ccatgctggt ggcctttaat
    16981 acctggctgt tgctgaaagc gtctttagaa acggcgctaa cagcgggttt gtggaatgca
    17041 ggatcgctca acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg
    17101 atgctcattg ccgccgcctt actggtacgc cggatgcgct tgctggaaat gggcgatgat
    17161 accgcgtgtg cgctgggcgt cagcgtcgaa cgttcgcgtc tgttaatgat gctggttgca
    17221 gtggtgctta ccgctgcggc aaccgcgctt gccgggccga tttcctttat tgctttagtc
    17281 gcgccgcaca ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg
    17341 ctgtgcgggg cgctgttact gctggcggcc gatctctgcg cccaacaact gtttatgccg
    17401 tatcaacttc cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg
    17461 ttaattcagg agtctcgcaa aaaatgaacg aatctgtagc ccgtttgcgc ggcgaacagt
    17521 taaccctcgg atatggcaaa tataccgttg cggaaaatct gactgtagaa atacctgatg
    17581 gtcacttcac ggcaattatc gggccaaatg gctgcggtaa atccacttta ctgcgtactt
    17641 taagccgcct gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac
    17701 attacgccag taaagaggtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc
    17761 caggcgatat caccgtgcag gagctggtgg cgcgtgggcg ttacccgcat caaccgctat
    17821 ttacccgctg gcgcaaagaa gatgaagaag cggtaacgaa agcgatgcag gccacgggaa
    17881 taactcatct ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgcgcgt
    17941 ggatcgcgat ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct
    18001 ggctggatat cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga
    18061 aaggctatac cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc
    18121 atttgattgc attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca
    18181 ctgctgaact gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag
    18241 ccggaacgcc gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa
    18301 ctaagtggtc tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca
    18361 cgctaccaca agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac
    18421 ccggaccgtc ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat
    18481 tcacatcgtt gatatttgct tttgttaact gctcaacaag atactgacga ttacgtaatt
    18541 caccgttcag ttccgcaacg tcagtgaccg ctttttcaat ttccagtttg cgttcaatac
    18601 cgtctgcgcc gagagaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt
    18661 atacgggctt tttaattccc gccgcgttgg caatgtcgag tgaataattg aggcgctgaa
    18721 tgtttgcatc aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa
    18781 actgggtttt gatttccagt ttattacgga cgttttctag tgactctttc accaccaacg
    18841 cagagatata atcgatatac ccgctcaaaa cggtctgcgc ctcttcactg gttggcgcgg
    18901 taaaacttag cgtccaggag gtatacagtg acggttcatc ttttttctta ctggcattgt
    18961 catcaaccgc tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat
    19021 cgattttcgc ctcttttaat tggtccatca cataaggtga tgaacgcagg tactcttcca
    19081 gcaagctaac cgactgaaac ttcttgataa acaggttaaa tgcttctgta cgatcaattt
    19141 tgatatccag atccagcact cgaagcttag tgaatgtttt ctccagctct tgccactgaa
    19201 caggttctgg aggcgtgaca accgccgcgc tggtccattt ttgcggcagg atgaaagaga
    19261 tcagcaagcc tgcgcaggca aacgcaaaaa tgaccgccat gaccgttttt ttggcccgcc
    19321 ataaaactgc gattagatta agtaaatcaa tttcattatt actgggcgac gccagaggat
    19381 actcaggaaa atgagcgtca cttccctgtt taatattcag tgatgacatg gggtataacc
    19441 tgaaaatgag tccaataggg gaaatctcca cggcgctcac tttaccagtt ggtgataatt
    19501 atccgaagct gaagtttgta aattccttcc actgaatgca acattcactc tatttaagag
    19561 aaatattcat caaacctata aatcaaacaa at
//
LOCUS       DAZGOB010000088        19413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGOB010000088 DAZGOB010000000
VERSION     DAZGOB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.088"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..185
                     /locus_tag="ACHLIF_003336"
     CDS             <1..185
                     /locus_tag="ACHLIF_003336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607298.1"
                     /translation="IYSSYPVHQKQKLTDAGLKLMATAKMPDALRLSGLRHLCNILNL
                     RAFVGRIRRSRRIRHE"
     gene            complement(355..731)
                     /gene="rnpB"
                     /locus_tag="ACHLIF_003337"
     ncRNA           complement(355..731)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHLIF_003337"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(764..1880)
                     /gene="garK"
                     /locus_tag="ACHLIF_003338"
                     /pseudo
     CDS             complement(764..1880)
                     /gene="garK"
                     /locus_tag="ACHLIF_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(1977..2867)
                     /gene="garR"
                     /locus_tag="ACHLIF_003339"
     CDS             complement(1977..2867)
                     /gene="garR"
                     /locus_tag="ACHLIF_003339"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO9607299.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2897..3667)
                     /gene="garL"
                     /locus_tag="ACHLIF_003340"
     CDS             complement(2897..3667)
                     /gene="garL"
                     /locus_tag="ACHLIF_003340"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO9607300.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3683..5017)
                     /gene="garP"
                     /locus_tag="ACHLIF_003341"
     CDS             complement(3683..5017)
                     /gene="garP"
                     /locus_tag="ACHLIF_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO9607301.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5392..6963
                     /gene="garD"
                     /locus_tag="ACHLIF_003342"
     CDS             5392..6963
                     /gene="garD"
                     /locus_tag="ACHLIF_003342"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO9607302.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWLDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDIASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7112..7447
                     /gene="prlF"
                     /locus_tag="ACHLIF_003343"
     CDS             7112..7447
                     /gene="prlF"
                     /locus_tag="ACHLIF_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO9607303.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7447..7911
                     /gene="yhaV"
                     /locus_tag="ACHLIF_003344"
     CDS             7447..7911
                     /gene="yhaV"
                     /locus_tag="ACHLIF_003344"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO9607304.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(7966..8775)
                     /gene="agaR"
                     /locus_tag="ACHLIF_003345"
     CDS             complement(7966..8775)
                     /gene="agaR"
                     /locus_tag="ACHLIF_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO9607305.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9024..10304
                     /gene="kbaZ"
                     /locus_tag="ACHLIF_003346"
     CDS             9024..10304
                     /gene="kbaZ"
                     /locus_tag="ACHLIF_003346"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO9607306.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCHDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10327..10800
                     /gene="agaV"
                     /locus_tag="ACHLIF_003347"
     CDS             10327..10800
                     /gene="agaV"
                     /locus_tag="ACHLIF_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO9607307.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10811..11590
                     /gene="agaW"
                     /locus_tag="ACHLIF_003348"
     CDS             10811..11590
                     /gene="agaW"
                     /locus_tag="ACHLIF_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO9607308.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11580..12458
                     /gene="agaE"
                     /locus_tag="ACHLIF_003349"
     CDS             11580..12458
                     /gene="agaE"
                     /locus_tag="ACHLIF_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO9607309.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12476..12910
                     /gene="agaF"
                     /locus_tag="ACHLIF_003350"
     CDS             12476..12910
                     /gene="agaF"
                     /locus_tag="ACHLIF_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO9607310.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLALQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12907..14040
                     /gene="nagA"
                     /locus_tag="ACHLIF_003351"
     CDS             12907..14040
                     /gene="nagA"
                     /locus_tag="ACHLIF_003351"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO9607311.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14391..15545
                     /locus_tag="ACHLIF_003352"
     CDS             14391..15545
                     /locus_tag="ACHLIF_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO9607312.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15558..16418
                     /gene="kbaY"
                     /locus_tag="ACHLIF_003353"
     CDS             15558..16418
                     /gene="kbaY"
                     /locus_tag="ACHLIF_003353"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO9607313.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16585..17061
                     /gene="agaB"
                     /locus_tag="ACHLIF_003354"
     CDS             16585..17061
                     /gene="agaB"
                     /locus_tag="ACHLIF_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO9607314.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17100..17903
                     /gene="agaC"
                     /locus_tag="ACHLIF_003355"
     CDS             17100..17903
                     /gene="agaC"
                     /locus_tag="ACHLIF_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO9607315.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17893..18684
                     /gene="agaD"
                     /locus_tag="ACHLIF_003356"
     CDS             17893..18684
                     /gene="agaD"
                     /locus_tag="ACHLIF_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO9607316.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18685..>19413
                     /locus_tag="ACHLIF_003357"
     CDS             18685..>19413
                     /locus_tag="ACHLIF_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO9607317.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQLLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHS"
BASE COUNT         4685 a         5076 c         4958 g         4694 t
ORIGIN      
        1 atatttattc atcttaccct gtgcaccaaa agcaaaaact cactgacgcg ggtttgaaac
       61 tgatggcaac cgcaaaaatg cctgatgcgc tacgcttatc aggcctacgc catctctgca
      121 atatattgaa tttgcgtgct tttgtaggca ggataaggcg ttcacgccgc atccggcatg
      181 aataaagcgc actcaacaat ctgaaacccg ccggaacggt ttattacgta catcaggtga
      241 aactgaccga taagccgctt tcttttgggt atagcgtcgt ggacagtcat tcatctttct
      301 gcccctccaa aagcaaaaac ccgccggttt acgctggcgg gttttcgtta aatcaggtga
      361 aactgaccga taagccgggt tctgtcgtgg acagtcattc atctaggcca gcaatcgctc
      421 actggctcaa gcagcctacc cgggttcagt acgggccgta ccttatgaac ccctatttgg
      481 ccttgctccg ggtggagttt accgtgccac ggactgttac cagccgcgcg gtgcgctctt
      541 accgcaccct ttcaccctta cctgatcccg cttgcgcggg ccatcggcgg tttgctctct
      601 gttgcactgg tcgtgggttt cccccccagg cgttacctgg caccctgccc tatggagccc
      661 ggactttcct cccctccgcc cgtctccccc gaagaggacg acgacgaagc ggcgactgtc
      721 tggtcagctt cggcgcgcag tatagagggt ttgcgcgccc ttgtcacccc gcgttgcgca
      781 ttccaatcgc cagtgtcgcg gcgatattac gtgaagcacg gcagatattg tcataagccc
      841 cgcggaatgc ttcgtccaac gtaccaatgc tggtcagtac gctgaagaca gcatcaatgc
      901 catgctgatg tacaacgcca acatcatcgg tcaggctacc cgcaatgcca atcaccggtt
      961 tatggtactt cttcgccacg tttgcgacgc caatcggtac cttgccgtga atactctggc
     1021 tgtcaatacg cccttcaccg gtgatcacca gcgtacaatc gtgaatatgc tcctccagat
     1081 tcagcgccgt agtgacgatt tcaataccac ttttcagttc cgcaccaaga aacgccatta
     1141 gcgccgggga catctttcac atcaacatgc agcgcttttt taatgacctc ggcatagtga
     1201 gagaggttat tgtccagctc aacaatcatc gcttcactgg ctcccttttg tgggccaaag
     1261 atgcgcgatg cgccgttatc gcccaccagc ggattggtga catcacaagc gacgcgaatg
     1321 acgcaatctt ttaagcgcgg atcgaggccg gaaatatcaa tatcattcag agtattaaga
     1381 ctaccgccgc caaaaccaat ttcattgccg ttggcgtcgc ataatttcgc ccccagcgcc
     1441 tgtaccatgc ctgcgccgcc atcatttgta gcgctaccgc caatgccgat aataatgttt
     1501 gtcgcgccgc tctccagcgc ctgcaggatt aactcgcctg tgccgcgtga agtggtcacg
     1561 agtggatcgc gtttttccgc aggtaccagc tccagcccac tggccgccgc catttcaata
     1621 aacgcggttt tgccatcgcc ggagatcccc caactggcat tcactttctc gcccagcggc
     1681 cctgtaaccc aggcgtgacg ttcagaaccc tgggtggctg caatcatcgc ttccaccgtt
     1741 ccttcgccac cgtcggcaac cggaatagaa acgtactgtg catcaggaaa aatttcccga
     1801 aatccttttt ctatcgcctg cgcaacctcg ctggcagata aactttcttt ataagagtct
     1861 ggggcgatta cgattttcat acctatgcct gttaccacat gacgccggag ggcgtttctc
     1921 ttattcggcc tggattccag gcccggattg caatacgcca tccgggcacg acgtcattaa
     1981 cgagtaactt cgactttcgc cagtttttcg tagtagcacg ccagggcgct atgatccgcc
     2041 gttcctaaac catctgctcg cagtgcctgc atcatctcca taaccgcagc tgtgagcggc
     2101 agttgcgcgc cgacgccgtg ggaagtatcc agcgcatttg ccagatcctt aatatgcaga
     2161 tcaatacgga agcccggctt gaagttgcgg tccatcacca tcggcgcttt ggcatccagc
     2221 acggtactgc ccgccagtcc accgcgaatt gcctgataca ccaggtccgg gttaacgccc
     2281 gctttggttg ccagcgttag cgcttctgac atcgcggcaa tattcagcgc cacaatgacc
     2341 tgatttgcca gtttggtgac gttacctgca ccgatttccc cggtatgcac cacggaaccc
     2401 gccatcgctt tcatcaaatc atagtatttg tcgaaaatag ccttgtcgcc gcccaccatc
     2461 actgacagcg taccgtcgat ggctttcggt tcaccgccgc tcaccggagc atccagcata
     2521 tcaatgcctt tcgctttcag cgcttcgctg atttcacggc ttgccagcgg tgcgatagaa
     2581 ctcatatcga tcaataccgt acctggcttc gcgccttcaa taatgccatt ctcacccagc
     2641 gccacctctt tcacatgagg ggagtttggc agcatggtta tgatgacgtc gcactgttca
     2701 gcgatcgctt tagccgtaga cgctgtttct gcacctgcag caatcacgtc agcaatagct
     2761 tctgggttac ggtcagcaac caccagcgag taacctgctt tcagaaggtt tttactcatt
     2821 ggtttaccca taatccccag gccaataaaa ccaactttca tagtcatatc aatcatctct
     2881 cttgttgcgg tggtggttat tttttaaagg tatcagccag tttctgagtg gcagagcgga
     2941 agacgccaag atcgctgccg acagccacaa acgtcgcgcc ccattccaga taacgacgcg
     3001 catcggcttc gaccggcgcg agaataccgc tgggtttgcc gtgcgcactg gcacggttaa
     3061 aaatgtgctg aattgctttt tgtacatccg ggtgcgaggc attgccgaga tggcctaatg
     3121 ccgcagccag atcgctgggg ccgacgaaga taccgtctac accttcggta gcggcaatgg
     3181 catcgacgtt atctacgccc tgctgacttt ctatctggac cagaatggtg atgttcttgt
     3241 tcgactgagc gaaataatcc gccacggtgc caaacatatt ggcgcggtga gaaacggaga
     3301 cgccgcgaat gccttccggt gggtaacggg ttgatgccac cgcctgctct gcttcctctt
     3361 ttgtttctac aaaaggaatc aggaagttat agaaaccgat atccagaaga cgcttaataa
     3421 ttaccggctc gttggtcggc actcgcacta ctggcgcgct ggcgctgcct ttcagggcca
     3481 ttaactgcgg aataaacgtg gagatatcgt ttggcgcatg ttcgccatcc agcaccagcc
     3541 agtcaaaccc agccaaacca agaacttcag tgctaatcgg gttagagagt gctgaccagc
     3601 aaccaatttg tacctgtttc gcagccagtg cggctttgaa tttattcggg aaaacatcgt
     3661 tattcatcgc ttataccttt gcttatttct gcaattccat acgtttaatg tcgccaacta
     3721 cgaagaggta gcagaccatc gccatcagcg ctgaacatcc cacgaaaacc agtgctgcat
     3781 tgaaggagtg cagttcactt accaggtagc caatcaccag tggagtgaca atggaggcaa
     3841 cattgccaaa gacgttaaag acgccgccac agaggccaac aatctctttc ggcgcggtgt
     3901 cagaaatcac cggccagccc agagcaccaa atcctttgcc aaaaaaagcc agcgccatca
     3961 gcatgaccac cagcgtggtg ttgttggtgt agttacataa gatgatggtg gaagccagca
     4021 acattcccag cacaatcggt agcttacgtg ccagggtcag ggataaaccg cgtttgatca
     4081 gataatccga gaagacacct cccagcacgc cgcccgcaaa accacacagt gccggaatcg
     4141 aggcgaccag acccactttc agaatcgaca tgcctttttc ctgcaccaga taaatcggga
     4201 accaggtgag gaagaaccag gtgatggtgt tgataaaata ttgtccgaaa aatacgccca
     4261 acatcatgcg gttagagagc aattgcttga tgtaatgcag tttgggaccg cttgctgccg
     4321 cgctgcccgg ctttttgtgg tccatatcga ccaccgcgcc attttcagag ataaacttca
     4381 gctcttccgc agacatacgt gggtgatctg tcgggttatg aatcaatttg atccacagcg
     4441 ccgtcagcac aaaaccaatc acccccataa cggtaaagac gtgctcccag ccccaggcga
     4501 aagtcagcca gccaagcaga ggcgaaaaga gcgccagcga gaaatattgc gccgagttaa
     4561 agatggcgga ggcagtacca cgttctttcg tcgggaacca ggcggcgaca atacgggcgt
     4621 tcgccgggaa tgatggcgct tccgagaagc cgagcataaa gcgcataaag aacatggaga
     4681 tccctgccca ggccagcggg aacatatcaa caaagccttg caggaaagtg aatagcgacc
     4741 agaaaaagag gctataggta taaacttttt tcgagccaaa cttatcaagc agccagccgc
     4801 cggggatttg catcagcaag taggcccagc caaaagcaga aaagatgtaa cccatcgaaa
     4861 ccgcacttaa ctgcaactct tttgccactt cggtaccagc aatagacagc gttgcacgat
     4921 cggcgtagtt aacggcggta acaataaaaa taatcagtaa tattaaatag cgggtatgca
     4981 cgcctttctt tttttcgtca acggtgtcca gaatcatttt atttacctcg ggtacttatg
     5041 ctgattttta ttattatggg gaaggtgtta tttatgagtt tcatttatgc cgtaacggca
     5101 atgaactcgg gaattagtat aagcagcgcg agaataataa tcattgtgca aatgctaatt
     5161 taattaatgc tatttaaata ttattttgag catatgcaca taaggttgcg cgctaaagca
     5221 cagatttgcg ctttaccttg ccgggcggca ctgcaatccc tgaaatgatt gacattgatc
     5281 acatttctgc gtttaaactc ctgacattct tatttcaccc aatgaagtca tttattttta
     5341 aatgagacca ggtcctcatt ttaataaccc ctggctggag aatatagtac aatggccaac
     5401 atcgaaatca gacaagaaac gccaactgcg ttttatataa aagttcacga cacagataat
     5461 gtggcaatta ttgttaatga taatggcctg aaagcaggaa cgcgttttcc ggatggactg
     5521 gaattaattg aacatattcc ccaggggcat aaagtcgcat tgctggacat tccggctaat
     5581 ggtgaaatta ttcgttatgg tgaagtgatt ggttacgccg tgcgtgcaat cccacgcgga
     5641 agctggctcg acgaatcaat ggttgtacta ccggaagcgc cgccgttaca cacgctgcca
     5701 ctggcaacca aagtcccggc ccccttaccg ccgctggaag gatacacctt tgagggctat
     5761 cgcaatgccg atggcagcgt gggcaccaaa aacctgctcg gcatcaccac cagcgtccac
     5821 tgtgtggcag gcgtggtgga ctatgtagta aaaatcattg aacgcgatct gctaccgaaa
     5881 tacccgaacg tcgatggcgt ggtggggctg aatcatttgt acggctgtgg cgtggcgatt
     5941 aacgcaccgg cggcagttgt gcctattcgt accatccaca atatttcgct gaaccctaac
     6001 tttggcggcg aagtaatggt gattggtctg ggttgtgaaa agttgcagcc tgagcgcctg
     6061 ctggtcggca cggatgatgt gcaagctatt ccagtagaaa gcgccagcat tgtcagtttg
     6121 caggatgaaa agcatgtcgg ttttcagtcc atggtcgagg atattttgca ggtcgccgaa
     6181 cgccatctac aaaaactgaa tcaacgtcag cgagaaacct gtccggcttc agaactggtt
     6241 gtcggcatgc agtgcggtgg cagcgatgcc ttttctggcg taacggcaaa cccggcggtt
     6301 ggctatgcgt ctgatctact ggtgcgctgc ggcgcaacgg tgatgttctc agaagtcacg
     6361 gaagtgcgtg acgcgatcca tctgctgaca ccacgcgcag tgaacgaaga ggtcggcaaa
     6421 cggctgctgg aggagatgga gtggtacgat aactatctca atatgggaaa aaccgaccgc
     6481 agcgccaacc cttcgccggg caacaagaaa ggcggtctgg caaacgtggt agagaaggca
     6541 ctcggctcca ttgctaaatc gggtaaaagc gcaattgttg aagtgctgtc gcccggtcaa
     6601 cgcccgacta aacgcggatt aatttacgcc gcgacgccag ccagcgattt tgtctgtggc
     6661 acgcaacagg tggcttcggg tatcaccgtg caagtgttta cgaccggtcg tggtacgccg
     6721 tacggcctga tggcggtacc cgtcattaaa atggcgaccc gcaccgagct ggcgaaccgc
     6781 tggtttgatt taatggatat taacgcgggc actatcgcca ccggcgaaga aaccattgaa
     6841 gaggtgggct ggaagttgtt ccactttatt ctcgacatcg ccagcgggaa gaagaaaacc
     6901 ttctcggatc aatgggggct gcataaccag ctggcagtgt ttaacccggc accggtgacc
     6961 tgatttcttt tggaacgtcc ctcgcaaaac atggccttag tgccatgttt ttattgttta
     7021 aagcccccac gtccattaat aatgcatttg cattacctta gttcaagctt ataatttgag
     7081 cagaaaacag gctgtaaaag gacagtgaat catgcccgct aatgctcgct ctcacgctgt
     7141 actgaccact gaatcaaagg tcacgatacg cggacaaaca actatccccg caccagtgcg
     7201 tgaggcctta aaactgaagc caggccagga cagcattcat tacgaaattc tgcctggtgg
     7261 gcaagtcttt atgtgccgac tgggagatga acaggaggat catactatga atgcattttt
     7321 gcgttttctg gatgcagata tccagaacaa cccgcaaaaa actcgtccat tcaacattca
     7381 acaaggaaag aaacttgtcg ctggcatgga cgtcaacatt gatgatgaga ttggcgacga
     7441 cgaataatgg attttccaca aagggttaat ggttgggcgc tatatgctca tccctgtttt
     7501 caggaaacct acgacgcttt agttgccgaa gtcgagacat taaagggaaa agatcctgaa
     7561 aattatcaga gaaaagccgc cacaaagtta ttggcggtag tccataaagt gattgaggag
     7621 catatcacgg tcaatccatc atcaccggca ttccgtcatg gcaagtcgtt aggctctggg
     7681 aaaaataaag actggtcacg ggtaaaattt ggtgctggtc gttatcgtct cttctttcgt
     7741 tatagtgaaa aagagaaagt catcattctg ggatggatga acgatgaaaa cactctgcgc
     7801 acctacggta aaaaaacaga tgcctatacc gtattcagca aaatgttaaa aagaggacat
     7861 cctcctgccg actgggaaac cctcacccga gaaacagaag aaacccattg atggtgttca
     7921 caatgcccgc cgaacgtatt cgtgttgacc agcgggcacc ctttctcact ccccgaccag
     7981 aatcacttca accccagcct ttcgcagtcc ttccagacta tccgcaggaa tgccttcatc
     8041 aacaatgatc atgtcgatac gttgagtatc aatgatctta tgtaaactgg aacgattgaa
     8101 tttactggaa tcggtgacca cgatgatccg ttccacaact tcgcacatcc gacggtttaa
     8161 acgggcttca tcttcattat gtgtgctgac gccgcgctcc agatcgatcg catctacacc
     8221 aagaaacagc atatcgaagt ggtaattttg cagcgattgc tcagcctgat cgccgtaaaa
     8281 agattgcgac tgacggcgca aatgcccgcc ggtcatcagc agctcaacgc cttccgcttc
     8341 cagcaacgca ttagccacgt tcataccgtt ggtcatcgca attacgtcag tgtgcttacg
     8401 catcagacga gcaatctcaa aagtggtagt cccggaatcg aggatcaccc gatgaccagg
     8461 ctgaatcaac tcaacggcag ctttcgcaac gctgcgtttc atcgcggtgt tcagcgcgct
     8521 tttatcttcc actgatggct cgactgacgg cgtcgtgcta tcgcagatca acgcgccacc
     8581 ataggcacgc acagcgatcc cctgcttttc cagaaacgcc agatcattgc ggatcgtcac
     8641 agtagatacg ccatacaatg ccgacagatc gttaacctgc acactccctt gctgtcgcag
     8701 acgctgaatg atctgttctc gtcgctcgct ggtgccagtc actcgcttct cacctgaagc
     8761 gtcggtatta ctcatagtaa gtcctttcgt aaaactttcg tttcatttcg ttttgcctat
     8821 taacgccttt ctattaagca aatgcaagcc caccttgccc attgacgcaa gctactctcg
     8881 tttcagtgac tttcattatg tttcttttgt gaatcagatc agaaaactat tatctttcgt
     8941 tttattttta tctcaccatg acgcagtatc aactgaaaca aaacgaaaga ttaatatcgc
     9001 agtaatctga actggagagg aaagtgaaac atctgacaga aatggtgaga cagcacaaag
     9061 cgggcaaaac aaatggaatt tatgccgttt gttccgcaca tccgctggtg ctggaggctg
     9121 caatccgcta cgccagtgca aaccaaacgc cgttactgat tgaagcaacc tccaatcagg
     9181 tagaccagtt cggcggttat accggaatga cgcccgccga ttttcgcggc tttgtttgtc
     9241 agctcgccga ctcgttgaat ttcccgcagg atgcgttgat tctgggtggt gaccatctgg
     9301 ggccaaaccg ctggcaaaac ctgccggccg ctcaggcaat ggccaatgcc gatgatttga
     9361 ttaaaagcta cgttgcggca ggattcaaaa aaatccacct tgattgcagc atgtcctgtc
     9421 atgacgatcc gattccctta actgatgaca tcgtggctga acgcgccgcc cgtctggcga
     9481 aagtggcgga agaaacctgt cttgaacact ttggcgaagc cgatctggag tatgtcattg
     9541 gtaccgaagt gccggtacct ggcggcgcgc atgaaacctt aagcgagctg gcggtcacca
     9601 cgccggatgc cgcccgcgcc acgctggaag cccatcgtca cgcctttgaa aagcaaggtt
     9661 tgaatgccat ctggccacgc atcattgccc tggtggttca acccggcgtc gaattcgatc
     9721 acaccaacgt tattgattat cagcccgcca aagcgagcgc cttaagccag atggtcgaaa
     9781 actacgaaac gctgattttc gaagcgcact ctaccgatta tcaaacgccg caatcgctgc
     9841 gccagctggt gattgaccac tttgccattc tgaaagttgg cccagcgctg accttcgccc
     9901 tgcgtgaagc tctgttctct ctggcggcga ttgaagaaga actggtgcca gcgaaagcct
     9961 gttctggtct gcgtcaggtg ctggaagacg tgatgctcga ccgcccggaa tactggcaaa
    10021 gccactacca cggtgacggc aacgcgcgtc gtctggcgcg tggttatagc tactcggatc
    10081 gcgtgcgcta ttactggccg gacagccaga ttgatgacgc tttcgctcat ctggttcgta
    10141 atctggcgga ttcaccaatt ccgctaccgc tcatcagcca gtatctgccg ctgcaatacg
    10201 tgaaagttcg ctccggcgag ctgcaaccga cgccacggga actcattatc aaccatattc
    10261 aggacatcct ggcgcagtac cacacagcct gtgaaggcca ataagcaaaa caaagaggaa
    10321 cacgctatgc caaatattgt tttaagccgg attgatgaac gcttgattca cggtcaggtc
    10381 ggcgttcagt gggtcggatt tgcgggggca aatctggtgc tggtagccaa cgatgaggtt
    10441 gccgaagatc cggtacaaca aaacctgatg gaaatggtac tggcagaagg gatcgccgta
    10501 cgtttctgga cgctgcaaaa agttatcgac aacattcatc gcgccgccga tcgacagaaa
    10561 atcctgctgg tttgtaaaac acccgccgat ttcctgacgc tggtgaaagg tggcgttccg
    10621 gtgaatcgca ttaacgttgg caatatgcac tacgccaatg gcaaacaaca aatcgccaaa
    10681 acggtttctg tggatgcggg cgatatcgca gcatttaacg acctgaaagc cgctggggtg
    10741 gaatgcttcg ttcagggcgt cccgacagag cctgctgtag acctctttaa attactttga
    10801 gggattcatc atggaaatca gcctgttgca ggcatttgcg ttgggcatta tcgcctttat
    10861 cgctggcctg gatatgttta acggcctaac acatatgcac cgcccggtgg tcctcggccc
    10921 gttggtcggg ctggtacttg gcgatctgca taccggaatt ttaaccggcg gtacgctgga
    10981 actggtgtgg atggggctgg ccccgctggc gggcgcacag ccgcctaacg tgattatcgg
    11041 tactatcgtc ggcacggcgt ttgccattac tactggcgtg aaacccgatg tcgcggtcgg
    11101 tgtcgccgta cctttcgctg tcgcagtaca gatggggatt accttcctgt tctcggtgat
    11161 gtctggcgtg atgtctcgct gcgaccggat ggcggagaac gccgatactc gcggcattga
    11221 acgcgtgaac tatctggcac tgctggcact cggcactttc tattttctct gcgctttcct
    11281 gcctatctac ttcggcgcgg aacatgcaaa aaccatcatt gatgtcctgc cgcaacgatt
    11341 aatcgacggc ctcggcgtcg cgggcggcat catgccagca atcggttttg ccgtgctgct
    11401 gaaaatcatg atgaaaaacg tctacatccc ctacttcatc ctgggctttg ttgccgcagc
    11461 ctggctcaag ttaccggtgc tggctattgc tgccgctgcg ctggcgatgg cgctgatcga
    11521 cctgctgcgt aaatctcctg aaccgactca acctgcggca cagaaagagg aatttgaaga
    11581 tggcatctaa tcaaaccacc ctgccgaacg tcagtgaaaa cgaagaaaca ttgctgactg
    11641 gcgtcaatga aaacgtgtat gaagatcaga gcattggcgc ggagctgacg aaaaaagata
    11701 tcaaccgcgt cgcctggcgt tccatgctgt tacaagcgtc ttttaactac gaacgtatgc
    11761 aggcttctgg ctggttgtac ggtctgctgc ctgctctgaa aaagatccac actaataaac
    11821 gcgacctggc gcgcgccatg aaggggcata tgggtttctt caatacccat ccgtttctgg
    11881 tgacatttgt tatcggcatt atccttgcga tggagcgttc taagcaggac gttaacagta
    11941 ttcagagcac caaaattgcc gtcggtgcgc cgctcggcgg gattggcgat gcaatgttct
    12001 ggctaacgct actgccgatt tgtggcggga ttggagccag tctggcattg caaggctcca
    12061 ttcttggcgc agtcgtcttt attctgctgt tcaacgtggt acacctgggg ctgcgttttg
    12121 gtctggcgca ttatgcttac cgcatgggcg tggcggcgat tccactcatt aaagcgaata
    12181 ccaaaaaagt cggccatgct gcgtctatcg ttgggatgac ggtaatcggt gcgctggtgg
    12241 caacctatgt tcgtttaagc accacgctgg aaatcacagc gggcgacgca gtggttaagt
    12301 tacaggctga tgttatcgac aaactgatgc ctgccttctt accgttggtc tacaccctga
    12361 ccatgttttg gttggtacgc cgcggctgga gtccgctgcg tctgattgca gttaccgtgg
    12421 ttctcggcat cgtcggtaaa ttctgccatt tcctttaaat acaatgaggt attagatgtt
    12481 aagtattatt ttgacagggc atggcgggtt tgccagcggc atggaaaaag caatgaagca
    12541 aattctcggt gagcaatcgc agtttatcgc catcgatttt ccggaaacct caagcaccgc
    12601 gctgcttaca tcgcagcttg aagaggccat cgcccaactg gattgtgaag acggcattgt
    12661 ttttctgacg gatttgctgg gcggcacgcc gtttcgcgta gcttcaacgc tggcgctgca
    12721 aaaaccgggc tgtgaagtga tcaccggcac caatttgcaa ctgctactgg agatggtgct
    12781 ggagcgcgaa gggttaagcg gtgaagaatt ccgtgtacag gcgctggagt gtggacatcg
    12841 tgggctgacc agtctggtgg acgagttagg ccgctgccat gaagaatgtc cggtcgagga
    12901 agggatatga cacacgttct gcgcgccaga aggctgctga ctgaagaggg atggctcgat
    12961 gaccatcagt tgcgtattgc tgacggtgtc atcgcagcaa tcgaaccgat tccagtgggc
    13021 gtgactgaac gcgatgcgga actgctctgc cccgcttaca tcgacaccca tgtacacggt
    13081 ggtgcgggcg ttgatgtaat ggatgacgcg ccggatgtac tcgacaagct ggcaatgcac
    13141 aaggcacgcg aaggtgtcgg cagttggttg ccgactaccg taaccgcgcc gcttaacacc
    13201 attcatgcgg cgctgaaacg tattgctcaa cgttgccaac gcggcggacc tggtgcgcaa
    13261 gtgctgggga gttatctcga aggaccgtac ttcacgccgc agaataaagg cgcgcatccg
    13321 ccggaattgt ttcgcgagct tgaaattgcc gagctggatc aattgattgc cgtttctcag
    13381 cacaccttac gcgtggtagc gctggcaccg gaaaaagagg gggcattgca ggccatccgc
    13441 catcttaaac agcaaaatgt acgagtgatg ctggggcata gcgcggcgac ctggcaacaa
    13501 actcgcgccg cgtttgatgc tggtgccgac ggcctggtgc attgctataa cgggatgaca
    13561 ggtttacatc accgcgaacc gggaatggtt ggcgcgggat taacggacaa gcgcgcctgg
    13621 ctggaactga tagccgatgg tcatcatgtg catccggcgg cgatgtcgct gtgttgttgc
    13681 tgtgcaaaag agagaatcgt gctgatcacc gacgcgatgc aggcagccgg gatgccggat
    13741 ggtcgctata cgttatgtgg cgaagaagtg cagatgcacg gtggcgttgt ccgtaccgcg
    13801 tccggtgggc tggcgggcag tacgctgtct gttgatgcgg cagtgcgcaa catggtcgag
    13861 ttgacgggcg taacgcctgc ggaagccatt catatggcat cgctgcatcc ggcgcgaatg
    13921 ctgggtgttg atggtgttct gggatcgctt aaaccgggca aacgcgccag catcgttgcg
    13981 ctggatagcg ggctgcatgt gcaacaaatc tggattcaga gtcaattagc ttcgttttga
    14041 tattttgctc ctttattggg ccttcacttc ccccgtaagg cctttctttt tctttcgttt
    14101 tgatctgtgc agcggtgtcg gatgcgacgc taacgcgtct tatgcgacct acggtacgcc
    14161 gcatccagca atcggtgaac atagcgacgt cattacgcaa ttctttaatc tccccttgtc
    14221 ttttctttca atgatcacag atttcgtttt atttcctttc tctccattga actttcggtt
    14281 tcttttctat agattttaat caacgaaaga catcaccaag tgaaatgaaa cgaaaggcaa
    14341 gtgaaagcga caacgcccga cgtcaagttc atcagactaa ggattgagtt atgccagaaa
    14401 attacacccc tgctgccgcc gcaaccggta catggactga agaagagatc cgccatcagc
    14461 ctcgcgcatg gatccgttca ctcaccaaca tcgacgcgct acgttctgcg ctcaataact
    14521 tccttgaacc gttactgcgc aaagagaatc tgcggatcat cctgaccgga gccggaacct
    14581 cggcatttat cggtgacatc atcgcgccgt ggctcgccag ccataccggt aaaaacttca
    14641 gcgccgtacc gaccaccgat ctggtcacca atccgatgga ctacctgaac ccagctcatc
    14701 cgctgctgtt gatctccttc ggtcgatccg gcaacagccc ggaaagcgtc gctgccgtgg
    14761 aactggcaaa tcaatttgta ccagaatgct atcacctgcc gatcacctgc aacgaagcgg
    14821 gcgctcttta ccaaaacgcg atcaacagcg ataacgcgtt tgccctgctg atgcccgcag
    14881 aaacgcacga tcgcggcttt gcgatgacca gcagcattac caccatgatg gccagctgcc
    14941 tcgcggtttt cgcacctgag acgatcaaca gccaaacctt ccgcgacgtg gcggatcgtt
    15001 gccaggcgat cctgacctca ctgggcgatt tcagcgaagg tgtgtttggt tacgcaccgt
    15061 ggaaacggat cgtttatctc ggcagcggtg gcttacaggg cgcagcacgc gagtcggcgc
    15121 tgaaagtgct ggaactgact gcgggtaaac tggcggcctt ctatgattcc ccgaccggat
    15181 tccgtcatgg cccgaaatca ctggtcgata acgaaacgct ggtggtggta tttgtctcaa
    15241 gccaccctta cacccgtcag tatgatcttg atctgctggc tgaactccgc cgtgacaacc
    15301 aggcaatgcg cgtaatcgcc atcgccgcgg aaagcaccga catcgtcgct gccggtccac
    15361 atattatcct gccgccgtca cgtcacttta tcgacgttga gcaggcattt tgcttcctga
    15421 tgtacgccca gacgtttgca ctgatgcagt cgctgcacat gggcaatacg ccggataccc
    15481 catcagccag cggcaccgtt aaccgcgtgg tgcaaggcgt aatcattcat ccgtggcagg
    15541 cataagagga tcgcattatg agcattatct ccactaaata tctgttacag gacgcccagg
    15601 ccaatggcta cgcggtgcct gcttttaaca ttcataacgc cgagacgatc caggcgatcc
    15661 tcgaagtgtg cagtgaaatg cgatcgccgg tgatcctcgc cggaacgccg gggaccttta
    15721 aacatatcgc gctggaagag atctacgccc tgtgcagcgc ctattccaca acctacaaca
    15781 tgccactggc gctgcatctc gaccaccacg aatcgctgga tgatattcgc cgtaaagtcc
    15841 acgcaggtgt gcgcagtgca atgatcgacg gcagccactt cccgtttgcc gagaacgtga
    15901 agctggtgaa atcggttgtt gacttctgcc actcacagga ttgcagcgtg gaagcagaac
    15961 tgggccgcct gggcggtgtt gaagatgaca tgagcgttga cgccgaaagt gcattcctga
    16021 ccgatccaca agaagctaaa cgctttgtcg aactgactgg cgtcgacagc ctggcggtag
    16081 cgattggtac ggcgcacggc ttatacagca aaacgccgaa gattgatttc cagcggctgg
    16141 cggaaattcg tgaagtggtg gatgttcctc tggtgctaca tggtgccagc gatgttccgg
    16201 atgaatttgt ccgtcgcact attgaacttg gcgtcacaaa agtgaacgtt gccacagaat
    16261 taaaaatagc cttcgctggc gcggttaaag cctggtttgc ggaaaatccg cagggtaatg
    16321 atcctcgtta ttatatgcgc gtcggaatgg atgcgatgaa agaagttgtc agaaataaaa
    16381 ttaatgtctg tggttcagcg aatcgaattt cagcataatc attagttttt tatgatttat
    16441 cccaatgtac ttcccgatta atatcgggga gtgccttaat ggaaaaggag atgactaaac
    16501 cttaataaat acatcagtac aatatcgcaa caataatata ttaaaaaaaa ttatattatt
    16561 caactttatg gtgaggatta cacaatgagc agtccaaata ttctcttaac ccgtattgat
    16621 aaccgtctgg ttcacggtca ggttggcgtg acctggacat ccaccatcgg tgcgaatctg
    16681 ctggtagtcg tggatgatgt tgtcgctaac gatgatattc aacagaaatt aatgggtatt
    16741 accgcagaaa cctacggctt tggcattcgt ttctttacta tcgaaaaaac cattaacgtc
    16801 atcggcaaag ctgcaccgca tcagaagatc ttcctgattt gccgtacgcc acaaacggta
    16861 cgtaaattgg tagaaggtgg cattgacctg aaagatatca acgtcggcaa tatgcatttc
    16921 tcggaaggga aaaagcaaat cagcagtaaa gtttatgtcg atgaccagga tctcgcggac
    16981 ttacgtttta ttaaacaacg tggcgtgaat gttttcattc aggacgtccc tggcgatcaa
    17041 aaagaacaaa tccctgacta aatctaaaat cgccttaata ttggtttgag gtaataaaaa
    17101 tgcatgaaat aaccctactt cagggattat ccctggcggc gttagttttc gttctgggga
    17161 ttgatttttg gctggaagcc ttatttttat tccgcccgat aatcgtttgt accctaactg
    17221 gcgctattct cggtgatatt cagactggct taattaccgg tggtctgaca gagttggctt
    17281 tcgccggatt aacccctgca ggtggtgttc agccgcccaa cccgattatg gcgggtctga
    17341 tgaccaccgt cattgcatgg tctacgggcg ttgatgccaa aacagcaatt ggtcttggcc
    17401 tgccgtttag tttgttaatg cagtacgtca ttctgttctt ctattccgct ttctcattat
    17461 ttatgaccaa agccgataaa tgcgcgaaag aggcggatac ggcagcgttt tcccggctta
    17521 actggacaac gatgctcatc gtcgcttcag cgtatgcggt gattgctttc ctctgtactt
    17581 acctggcaca aggggcgatg caggcgctgg tgaaagcgat gcccgcctgg ttgacccacg
    17641 gctttgaagt ggctggcggt attctgcctg ccgttggttt tggcttgctg ctgcgcgtaa
    17701 tgttcaaagc gcaatatatc ccttacctga tcgccggttt cctgtttgtt tgctacatcc
    17761 aggtcagcaa cctgttgccg gttgccgtac tgggcgcagg ctttgcggtg tatgagtttt
    17821 tcaatgcgaa atcccggcag caagcgcaac cgcagcccgt tgccagtaaa aatgaagaag
    17881 aggactacag caatgggatc tgaaatcagt aaaaaagata tcacccgtct gggctttcgt
    17941 tcgtcgctgc tgcaagcgag ctttaactac gaaaggatgc aggcgggcgg ttttacctgg
    18001 gcgatgttgc cgatcctgaa aaagatttat aaggacgaca aaccgggctt aagcgcggcg
    18061 atgaaagata acctcgaatt tattaatacc cacccgaatc tggtcggatt cctgatgggg
    18121 ttattaattt cgatggaaga aaaaggagaa aaccgcgaca ccattaaagg cctcaaagtg
    18181 gcactgtttg gcccaatcgc cgggattggc gatgcgattt tctggtttac tttgttgccg
    18241 attatggcgg gaatttgctc atcatttgcc agccagggaa acctgctggg gccgattcta
    18301 tttttcgccg tttacctgct tatctttttc ctgcgcgtcg gctggaccca cgtcggttat
    18361 tcagtcggcg tgaaggcgat cgataaagtg cgagagaact cgcagatgat tgctcgctcg
    18421 gcaaccatcc tcgggatcac ggtaatcggc gggctgatcg cttcgtatgt gcatattaac
    18481 gtggtgacat cgtttgccat cgacagtacc cacagcgtcg cgctgcaaca ggatttcttc
    18541 gataaagtct tcccgaacat tttaccgatg gcctacaccc tgctgatgta ttacttcctg
    18601 cgggtgaaaa aagcgcatcc ggtgctgtta atcggcgtga cttttgtgct ctctattgtt
    18661 tgttccgcat tcggcatttt gtaaatggaa cgaggcactg cttctggcgg tgcctcttta
    18721 cttaaggaat ttcatcctgt gcaaaccctt cagcaagttg aaaactatac ggcgttaagt
    18781 gaacgagcca gcgaatattt attggctgtg atccgtagca aaccggatgc cgtgatttgc
    18841 ctggcgaccg gagccacgcc attactgacg tatcattatc tggtagaaaa aatccaccag
    18901 cagcaggttg atatcagcca gctcaccttc gtgaagctcg acgaatgggt ggatctgcca
    18961 ttaacgatgc ctggcacctg cgaaactttc ctacaacagc atatcgtgca gctgctgggg
    19021 ctgcgtgaag accagctcat cagctttcgc tccgaagaga taaatgagac agagtgcgaa
    19081 cgggtaacga acctgattgc gcgcaaaggc ggtctggatt tatgcgttct aggattgggg
    19141 aaaaacggtc atcttgggct gaacgaaccg ggagaaagcc tacaaccggc ctgccatatc
    19201 agtcaacttg atgccagaac acagcaacat gagatgttaa aaaccacggg tcgccccgtg
    19261 actcgtggga tcaccttagg cctgaaggat attctcaatg cccgcgaggt tttgttattg
    19321 gtgactggcg aaggaaagca ggatgcgaca gaacgttttc tcacggctaa agtctccacc
    19381 gctatcccgg cttcgttttt atggctgcac agt
//
LOCUS       DAZGOB010000089        18959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGOB010000089 DAZGOB010000000
VERSION     DAZGOB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.089"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            144..710
                     /gene="yqaB"
                     /locus_tag="ACHLIF_003358"
     CDS             144..710
                     /gene="yqaB"
                     /locus_tag="ACHLIF_003358"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO9607318.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKYHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            707..1135
                     /locus_tag="ACHLIF_003359"
     CDS             707..1135
                     /locus_tag="ACHLIF_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO9607319.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1208..2764
                     /gene="gshA"
                     /locus_tag="ACHLIF_003360"
     CDS             1208..2764
                     /gene="gshA"
                     /locus_tag="ACHLIF_003360"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO9607320.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            2914..3429
                     /gene="luxS"
                     /locus_tag="ACHLIF_003361"
     CDS             2914..3429
                     /gene="luxS"
                     /locus_tag="ACHLIF_003361"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO9607321.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHLIF_003362"
     CDS             complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHLIF_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO9607322.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHLIF_003363"
     CDS             complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHLIF_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO9607323.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHLIF_003364"
     CDS             complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHLIF_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO9607324.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHLIF_003365"
     CDS             complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHLIF_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO9607325.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHLIF_003366"
     CDS             complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHLIF_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO9607326.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8154..9338)
                     /locus_tag="ACHLIF_003367"
     CDS             complement(8154..9338)
                     /locus_tag="ACHLIF_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9607327.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     LLGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFINDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHLIF_003368"
     CDS             complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHLIF_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO9607328.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHLIF_003369"
     CDS             complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHLIF_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO9607329.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHLIF_003370"
     CDS             complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHLIF_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO9607330.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHLIF_003371"
     CDS             complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHLIF_003371"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO9607331.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHLIF_003372"
     CDS             complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHLIF_003372"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO9607332.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYPNQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHLIF_003373"
     CDS             complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHLIF_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO9607333.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHLIF_003374"
     CDS             complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHLIF_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO9607334.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17180..17509)
                     /locus_tag="ACHLIF_003375"
     CDS             complement(17180..17509)
                     /locus_tag="ACHLIF_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO9607335.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17661..18005
                     /locus_tag="ACHLIF_003376"
     CDS             17661..18005
                     /locus_tag="ACHLIF_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO9607336.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18042..18491)
                     /gene="alaE"
                     /locus_tag="ACHLIF_003377"
     CDS             complement(18042..18491)
                     /gene="alaE"
                     /locus_tag="ACHLIF_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO9607337.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPANILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         4752 a         4780 c         4896 g         4531 t
ORIGIN      
        1 ctcccaaaaa tttccttcaa aaatatctca tttctaaata actactttat ccatttcctg
       61 aattttcttc tccttcgcca tcagcgacaa aatcaagcaa ttacgctaaa gtagcgtcat
      121 atttttcgac ttgcgagaag accatgtacg agcgttatgc aggtttaatt tttgatatgg
      181 atggcacaat cctggatacg gagcctacgc accgtaaagc gtggcgcgaa gtattagggc
      241 actacggtct tcagtacgat gttcaggcaa tgattgcgct taatggatcg cccacctggc
      301 gtattgctca ggcaattatt gagctgaatc aggccgatct cgacccgcat gcgttagcgc
      361 gtgaaaaaac agaagcagta agaagtatgc tgctggatag tgtcgagccg cttcctcttg
      421 ttgaagtggt gaaaagttgg catggtcgtc gcccaatggc tgtaggaacg gggagtgaaa
      481 gcgccatcgc tgaggcattg ctggcgcacc tgggattacg ccgttatttt gacgccgtcg
      541 tcgctgctga tcacgtcaaa taccataaac ccgcgccaga cacatttttg ttgtgcgcgc
      601 agcgtatggg cgtgcaaccg acgcagtgtg tggtctttga agatgccgat ttcggtattc
      661 aggcggcccg tgcagcaggc atggacgccg tggatgttcg cttgctgtga gtgaagcgtt
      721 atcgcttttc tcgttgtttg ccagtagttt tctcagcgct acactattac ccggcaactc
      781 cgaagtcgtg ctggtggcaa tgttgctttc cgggatcagt tatccctggg ttttagtttt
      841 aacagcaaca atgggtaata gccttggagg gttaactaac gttatccttg ggcgtttctt
      901 tccattgcgt aaaacatcgc gctggcaaga gaaagctacc ggctggctga aacgctatgg
      961 tgcagtcacg ctattattaa gctggatgcc cgtggttggc gatttactgt gtctgttagc
     1021 gggatggatg cgcatctcct gggggccagt aatctttttt ttgtgccttg gtaaagcgtt
     1081 acgctatgtt gcagttgcag cagcgaccgt tcagggcatg atgtggtggc actaattgta
     1141 ggcctgcaca tatggtcacc attacagtta tgctaattaa aacgattttg acaggcggga
     1201 ggtcaatttg atcccggacg tatcacaggc gctggcctgg ctggaaaaac atcctcaggc
     1261 gttaaagggg atacagcgtg ggctggagcg cgaaactttg cgtgttaatg ctgatggcac
     1321 actggcaaca acaggtcatc ctgaagcatt aggttccgca ctgacgcaca aatggattac
     1381 taccgatttt gcggaagcat tgctggaatt cattacacca gtggatggtg atattgaaca
     1441 tatgttgacc tttatgcgcg atctgcatcg ttatacggcg cgcaatatgg gcgatgagcg
     1501 gatgtggccg ttaagtatgc catgctacat cgcagaaggt caggacatcg aactggcaca
     1561 gtacggcact tctaacaccg gacgctttaa aacgctgtat cgtgaagggc tgaaaaatcg
     1621 ctacggcgcg ctgatgcaaa ccatttccgg cgtgcactac aatttctctt tgccaatggc
     1681 attctggcaa gcgaagtgcg gtgatatctc gggcgctgat gccaaagaga aaatttctgc
     1741 gggctatttc cgcgttatcc gcaattacta tcgtttcggt tgggtcattc cttatctgtt
     1801 tggtgcatct ccggcgattt gttcttcttt cctgcaagga aaaccaacgt cgctgccgtt
     1861 tgagaaaacc gagtgcggta tgtattacct gccgtatgcg acctctcttc gtttgagcga
     1921 tctcggctat accaataaat cgcaaagcaa tcttggtatt accttcaacg atctttacga
     1981 gtacgtagcg ggccttaaac aggcaatcaa aacgccatcg gaagagtacg cgaagattgg
     2041 tattgagaaa gacggtaaga ggctgcaaat caatagcaac gtgctgcaga ttgaaaacga
     2101 actgtacgcg ccgattcgtc caaaacgcgt tacccgcagc ggcgagtcac cttctgatgc
     2161 gctgttacgt ggcggcattg aatatattga agtgcgttcg ctggacatca acccgttctc
     2221 gccgattggt gtagatgaac agcaggtgcg attcctcgac ctgtttatgg tctggtgtgc
     2281 gctggctgat gcaccggaaa tgagcagtag cgaacttgcc tgtacgcgcg ttaactggaa
     2341 ccgggtgatc cttgaaggtc gcaaaccggg cctgacgctg ggtatcggct gcgaaaccgc
     2401 gcagttcccg ttactgcagg tgggtaaaga tctgttccgc gatctgaaac gcgtcgcgca
     2461 aacgctggat agcattaacg gcggcgaagc gtatcagaaa gtgtgtgatg aactggttgc
     2521 ctgcttcgat aatcccgatc tgactttctc tgcccgtatc ttaaggtcta tgattgatac
     2581 tggtattggc ggaacaggca aagcgtttgc agaagcctac cgtaatctgc tgcgtgaaga
     2641 gccgctggaa attctgcgcg aagaggattt tgtagccgag cgcgaggcat ctgaacgccg
     2701 tcagcaggaa atggaaaccg ctgataccga accgtttgcg gtgtggctgg aaaaacacgc
     2761 ctgacagaaa agaaaaaggc cactcgtgag tggccaaaat ttcatctctg aattcaggga
     2821 tgatgataac aaatgcgcgt ctttcatata ctcagactcg cctgggaaga aagagttcag
     2881 aaaattttaa aaaaaattac cggaggtggc taaatgccgt tgttagatag cttcacagtc
     2941 gatcataccc ggatggaagc gcctgcagtt cgggtggcga aaacaatgaa caccccgcat
     3001 ggcgacgcaa tcaccgtgtt cgatctgcgc ttctgcgtgc cgaacaaaga agtgatgcca
     3061 gaaagaggga tccataccct ggagcacctg tttgctggtt ttatgcgtaa ccatcttaac
     3121 ggtaagggtg tagagattat cgatatctcg ccaatgggct gccgcaccgg tttttatatg
     3181 agtctgattg gtacgccaga tgagcagcgt gttgctgatg cctggaaagc ggcaatggaa
     3241 gacgtgctga aagtgcagga tcagaatcag atcccggaac tgaacgtcta ccagtgtggc
     3301 acttaccaga tgcactcgtt gcaggaagcg caggatattg cgcgtagcat tctggaacgt
     3361 gacgtacgca tcaacagcaa cgaagaactg gcactgccga aagagaagtt gcaggaactg
     3421 cacatctagt cagtaaacta tcttcacaat taattgaaaa aagccagttc aaatgaactg
     3481 gcttagttgt acttagtgcg caccgcctcc gccgccacct gcgccaaatg gcggtttagc
     3541 aaaccacacc agccccagca gtacgaggaa tattccggct gacatccaga agatctcatt
     3601 ggcggaaata atcagcccct gattggtgat ctgctgggca atccagcctg atgcctgctg
     3661 ttgcgtcatc ccaagccctt ccagttgact gtacatcgcc tgggcattcg ggttgaacgg
     3721 gtttaccgac tcagtcaact gcgcatggtg catcgactcg cggttggtcc acatggtcgt
     3781 ggttatcgac gtgccgatag accccgccag cgttcgcgta aagttagaga ggctcgatgc
     3841 cgccgccagt cgttccggtg gcaaaccaga cagcgtaatg gtggtcagcg gcataaagaa
     3901 gcaggccacc gcaaacccct ggataaactg cggccaggcc gacgcgccaa aatccatacc
     3961 tggttcaaag gtataggcac gccagtagaa gcagacggca tacataataa agctgaaggt
     4021 taccagccgc cgcatatcca gtttatgcgc gaagcggccg ataatcggcg acaggatcac
     4081 cggaataatc cctaccggcg cagaggccaa acctgcccag gtcgccgtgt aaccgtagac
     4141 ctcctgcaac aactgcggca gcagaacaat agcgccgaag tagagcatat acgcgaggct
     4201 gatacacaag cagccgatgg tgaagttgcg cgacttaaac aacgacagat cgactatcgg
     4261 gttatcgtcg gtcagctccc agacaatcag gaagcagata gccaccaccg ccaccacggt
     4321 aaggatgata atttcctgtg atgaaaacca atccagctct ttaccgcggt caagcataat
     4381 ctgcaggctg ccgataccaa taaccagcag tgccagcccc acggcatcaa tccgccgccg
     4441 ttcggtgcgg gtttcacgtc cgcgcagagt ttgcagtgtc atcaacacca ccgccacgcc
     4501 aatcggcacg ttgatgaaga atatccagcc ccagtggtaa ttatcgctga tataaccgcc
     4561 gaggatcggg ccgcaaattg gcgcgacaat caccgtcatc gaccacaacg ccagcgcgat
     4621 cgagcgtttg gctggcgggt agttattcag caatagactt tgcgaaagcg ggatcaacgg
     4681 cccggcgaca atcccctgaa tcacgcggaa gaagatcagc atattcaggc tgctggagac
     4741 accacacgcc cacgacgcaa tagcaaaggc gatggtggac caaaggaaca gtttcacttc
     4801 cccgacgcgc tttgccagcc agccggtaag cgggatcgag atggcattcg ccaccccgaa
     4861 agaagtgatt acccacgttc cctggctgag cgatgagccc agattcccgg cgatagtggg
     4921 gatcgccacg ttagcaatgg tggagtccag cacctgcatg aatgtcgcca gtgacagcgc
     4981 aatcgtcata atgaccagtt gcgcgccttc cagcggtttt tgctgttgca tcacacgcac
     5041 ctctggatta gccagcgtta gcttttacga tatcgtcgat cagtttattg acaggtgcca
     5101 ggctgatttc acgcgcggta cttaccgcga ccggtgttga acgtacttta tttgccagta
     5161 cctgaccgtc acggttagtg gtattgacgc tcaccagcgt ggacaaacca atgcgcagcg
     5221 ggtattgctc cagctgtttc tggtccagtt cgatacgcac aggtagacgc tgaacgactt
     5281 tgatccagtt accggtcgca ttttgcgccg gaagcagtga gaatgcgcta cctgtgccca
     5341 tatccagacc aaccacttta ccggtgtatt tcacatcatc gccgtaaata tccgtggtga
     5401 tagtgaccgg ctgaccgata cgcatattgg caatctgcgt ctctttaaag ttggcatcca
     5461 cccacatatt ggtggctgga acgaccgcca tcagcggcgt cgttgggcta atttgcgccc
     5521 caggctgtac cgcgcggcgg gagacataac cggtcatcgg actgacaata cgggtacgct
     5581 ccagcgccag ccaggcgtta cgtacttcgg tggcagcctg ttgcacggca ggctgatctt
     5641 ccagtttagt ccccagaatc atcgcctgat tggcattgta ttgttgaata gcgacgtcca
     5701 gttgcgcctg ggcactggtg acggcgtcgc gggcgtgttg cagctcttcg cgaccaatca
     5761 ggttggcatt gcccagcggc acacggcggt tgtagtcgct ttgtgctttc gcgagggcga
     5821 ttttttgcac ctcaatattc gcctgcaact gtttgctgtt aatcatcagc tggtgggttt
     5881 ggcgaacgct ggaagccagt gcagttttgg ctttttcaaa cgcctggcga gcatctgtcg
     5941 ggtcgagagt gaccagaaca tcgccttctt ttacaaaatc ggtgttatcg gcccagactt
     6001 tcgtcacgct gccagacacc tgagacataa tttgaatctg attccctgcc acgtatgcgt
     6061 catcggtttc ttcgaaatga cgcagtacca aaaaccaata aatccctatc gctacggcaa
     6121 taattataaa gagcaaggtg agaaggagga gcagacgctt acgtttgccg ctcttcttta
     6181 ccggttgctg cggggtttga gtctccgcat ttgcgctcat attgttctcc acgatcttct
     6241 taactcatcg gctgagccga gctgcttgcc aaaaaggcca gcatgttgcg atgctggccg
     6301 gtcatttttt cttttataaa tctggatttt tgagcgagat gacgcgttag ctcatcgctt
     6361 cgagaaccac accgtcttgt tccatctggt cgagacggga gagcaatttg cgggtgattt
     6421 gctcgagctg atctttttct gttgtgctga gcgcggacca gagttgatgc aggcagttat
     6481 gctgcggtgg taaaacctcg cgcaaaaact cgtgaccttt ttccgttaat tgcagatgca
     6541 ggcagcggcg atcgttatcg ctttcacgac gttcgatcca accgcgtttt tccagttcat
     6601 cggcaatacg cgtcgcgttg gtacgggatg atccaagagc acaacttaat tcagaaggct
     6661 gaatactgtg gttttcctga gactccagcg taatcaacgc cataaacaac gtctcgttaa
     6721 tcccctgagc cttcagcatt ttattgcggt tctccagcag cttgctttgc atgtgcatgc
     6781 aaagacgagt cagaaggatc tcctgataag gaaaatcttc gtggcggctg gcgcgaaatt
     6841 ttagcatttg ttcaatgggc gtaaacgaac tatccatttg ggtatgacct cattaatttc
     6901 agccgatata gtaacgacag tgacaaataa agtaaatgta ttgttttaga aaaatgattc
     6961 ttgtgggtta tataatcgcc atcactttcc aggcgagccc ataggccagc gcactaagca
     7021 gtgttgggat gataatgctg cgtgttttat agaaactggc acccagtacc gcgaagccga
     7081 ccagcgtggg cacgaaacgg cgtgtatcgt gcatcacttc tggtgcggta gagacaacca
     7141 gcagagcgca tatcgaggcg atgccaatgg tgtcgagcaa aatacctacc gcgccacgtt
     7201 tggttgggcg ggcattaccc acacgcaggc gcagcggcaa atagcggaag caataattcg
     7261 ccacgccaac cagtaaccca agcagcagaa cctcatagct catcgggcgc tccttgccag
     7321 aatgcctgga ttaacgcagt gaggcagcca cagacaatgc ctgccagaat ggcgacggga
     7381 atagaaaata gcgttacgcc tgcaagggca ccaactaacg ctgcggtaac gcaaagagat
     7441 tgtttgcgct ggaaagaggc gagcaggaaa ctcataaaga gtgccggaag cataaaacct
     7501 aatgcagctt caacggcggg ataaccttgc agcaagccgc tgccggagaa tgcccctatt
     7561 accgtaccaa atacccacga tgaccatgaa ctgaaggcaa tgccgatcat ccagttctcg
     7621 ctccagcggc gattattgcg taccagtttt gcggttgcgg cggcaaaaac ctcatccgtc
     7681 aggccaaatg cccacagggc ggttttcgat ttttgcagac gctgaataat acggctacgc
     7741 agtgacgggc catacaacac atggcgaaca tccattgcca tgacggtcag tgcagcaacc
     7801 cacaaactac tcccggctgc cagcatcgcg gtaatgacga actggctcgc gcctgcataa
     7861 atgatgcagg agaaaaaaac gctttcgaga ggagagaatc ccagacgggt cgcattcaga
     7921 ccgaacgcaa aggccaccgg aatataacta ataacaatcg gtaaactgtc tttgcatcct
     7981 tccatgaagg tcgccgaacc aggagcaggc tgtggagtag ggctttccat agagtgtacg
     8041 cttaacgatt gttaatatta taaatagact gaatgaatat cttaacctta tcagactgat
     8101 gggcttctta acacccttat aagtgtaaag ccacgaaaac ggttgctgat tgcttaattt
     8161 gcggcttcct gacgatgaaa acctcgccac cagaccagta agttaaccag ggcaatcgtc
     8221 gcgccagcca gacaaacgcc agcccaaccg ccatgttgcc aggctgaggc tgaaattagc
     8281 gaaccggcgg caccgccaat aaagtagctg gtcatgtaac ctgcggtcag gcgattacgc
     8341 gcatcagggt gtattcgata aattaccgtc tggttagtga tatgcacgcc ctgtacggtg
     8401 agatccagca ccaggattcc gataatcaac gccagtacgg aagtgtgacc aaaccagatc
     8461 gccagccatg aaagtaatag cagcagcaga ccgaaagttg tggtgtggtg cgatttaccc
     8521 ttatcggcaa aaccgcccgc cggacgagcg cccaacgcgc cggcagctcc cgcaagtcca
     8581 aacagaccaa tgacaccatc gctgtagtta aaaggtggag cggcaagcaa aaaggccatt
     8641 gaggtccaga gaatgctgaa gttggcaaag gtcaggcagc caagcaatgc gcgggtgcgc
     8701 aggatcttat cgttgataaa catgctgaaa acggaaccca acaactgtgg gtagttgagg
     8761 tgggtttctg atttcatttg tggcagacca cgccataatg ctagcgccat cagagccatt
     8821 aacaccgagg caacccaaaa gacggtgcgc cagccgccga ggtttgccag taacccggca
     8881 acggttcgtg ccagcaatat ccccaacagc agaccgctca taatggtgcc aaccactttg
     8941 ccgcgtttat ccggtgaagc cagcgtcgct gccagcggaa ccagaatttg tgccacgaca
     9001 gagaataaac cggttaatgc cgtaccgagg agcatcatcg ccagcgactg actgctggcg
     9061 gtaatcaaca taccgcccgc tgccagtaag gtcatcgaga caatcaggcg gcggcgttca
     9121 aacatatcac cgaggggaac aagaaacagc agacctgctg catagcccaa ttgcgcggcg
     9181 gtaacaataa agcctgccga actggcggaa agggaaaagt tacgcgcgat ggtgtcgagt
     9241 agcggctggg cgtaatagtt actggcgacc gccagaccgg tggcgataga catcagcacg
     9301 atcagcgccg ggctaagctc atgattaggt ttagtcatta tttttgttca tcaggtgatg
     9361 attaagctgg aaatcaataa tagcggatgt tgatgaatat gggatgaaag atagcttgtt
     9421 gaaatgtgcg atgtggtttg taggcatgat aagacgcgtc agcgtcgcat caggcattgt
     9481 gtacagagcg gcggatgagg caaaaacgcc ttatccgccc gaataaaaat tacttctgcg
     9541 ctgccagcgc ctcattcacc cagccatcga actgctgctg gtgggctttg atccagccat
     9601 caacatggcc ctgaatatcg ccttctgagg ctttgccgtc atgcataatg gcgttctggg
     9661 cgttaatatc tgccactggc aactgcataa tggcaaacag tttcgctgct gccgggtttt
     9721 tctcggccca ggctttgttg gcaacgatat gcatggtgct gaccgggaag ccataattcg
     9781 caccattcgg cagtttggta tcggcgtttt tatcgcccgg cagtgcggag aacggcacct
     9841 gcaaccagac cacatctttc cctggcttca gctcattact cacccagtac ggcgtccagg
     9901 tgtagtaaaa caccggtttg ccctctttgt agcgactgat ggtgtcggcc atcatcgccg
     9961 cgtagttccc ctgattatgc gtcacggtat gggtcagttc atacgcggca agctggtggt
    10021 tgatcgcacc ttcgcagccc cagccagggt tacaaccggt taaatccgct tttccgtcgc
    10081 cgttggtatc gaacagtttg gcgatcttcg gatctttcag ttgtgcgatg ttggtgattt
    10141 tgtactggtc ggcggttttc ttatcgatca ggtaaccctg tgccgcgccg ttaacaaata
    10201 ccccttcacg ataaaatttc ttatcgccac cggcagcttc gtacatgttg tcatgcagtg
    10261 gcgtccagtt cacggcggtg aaggttgcat cgccggaagc aagcgaggtg tagccaacgt
    10321 tgtaatccac ttcgctgggt ttgttgacgg tataacctaa tttctccagc gcacgactga
    10381 ccagcagcgt ctggaaggtt tcttcagtga tggtgctctg aactggatta acagtaatgc
    10441 ctttgcccgg cagatcggca gcaaaagttt gtgtagagat aagcgtggca aacgctgtcg
    10501 caaaaagtac gctatgtcgc attgttattc ctttttttgg cagggtgata atgcccggcg
    10561 tcaccgggca agtgcagagt tacttaatga atgggcgggt cagcagacca acagggccag
    10621 tggtgtacca gcgacggttg ccgcgactgc gtgagtcgcg cccaacggcc tgcgtcaggc
    10681 gatcgaggat aatggcgagg atcacaatcc cgacgccgcc aacggtggca agccccatat
    10741 ccagacgacc gataccgcga agtaccatct gacccagccc gccgacggca atcatcgagg
    10801 caatgaccac catagaaagg gccagcatca gcgtctggtt aacgcccgcc ataatggtcg
    10861 gcatcgccag cggtaactga actttgaaca gcatctggcg cgggctggca ccgaatgagc
    10921 gcgaggcttc aatcagatcc gccggaacct ggttaatccc cagaatggtc agacggataa
    10981 tcggcggcag agcaaagatg atcgtcacca ccacgcccgg cacgttaccg ataccaaata
    11041 gcatgacgat tggcaccaga taaacaaacg ctggcgtggt ctgcatggca tcaagcagtg
    11101 gacgaataat tttcgccgct cgcggacttc tcgccagcca gatccccagc ggcaaaccaa
    11161 tgacgataca gaacagcagg gcggttaaca ccagcgccag ggtaaccatg gcctgcgacc
    11221 aggcaccgat tgcgccgatg gcaatcagcg aaaccagcgt cgccacgccc attccgaccc
    11281 cggaaatctg ccaggcgatg agagcgaaaa cgataatcgc caccggtgcg ggcataccca
    11341 gcagcaattg ctggaaaccg ttgagaatgt aatcaaccgg aacgcgcacg ccctggaaga
    11401 cgggacggaa atgggtaaca acccagtcga tcccttcagt gacccaactg tcgagcggga
    11461 tcagcgtttt atggaacgga tcgagaatat taaaatgctc gacgtttggc gcaggcgtac
    11521 tggtcagcca gtcagcacca ccgccgtcag tcggtgcagt cgccggtgta ccccaggcgt
    11581 ctgcggattg cacggcactg tccgccgctg gcgtggtatc ccacggatta ttttgatcag
    11641 ccattattta ccccctcacg atctaaagcg cgcagcagca ttcctttcga aatgatgccg
    11701 acatactgtt ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc gacatgagag
    11761 agcaactcgc taagaggcgt ttgtgcatcg actgctaacg gcgcatcaat cagcgccgca
    11821 tcaagacctt gctgctgcgt taacgcggtt ttaagcgaat cgatggaaac tgcgccgaca
    11881 aacttattac cgcgttcgat aacgtagcca tattcgcgat cttcatcctg caataatttc
    11941 agtgccgaac gtgggccgaa gccaggggtt ttacgaatta agccattcgg tgtccggcgg
    12001 gcaatatctt tcgcactgaa tacctgacta atatcaacgc cacggaagaa ggtacggaca
    12061 taatcattcg ccggattatt gagaatttca tccggtgtgc cgacctgtac cacttcacca
    12121 ttttgcataa tggcaattcg gtcgccaata cgcatagcct catcgagatc gtgggaaata
    12181 aagacaatgg tgcgctgatg tttcgcctgt aattttacca gctcatcctg catctcggtg
    12241 cgaattaatg gatcgagcgc cgagaaggct tcgtccatta ataatatatc cggattaatc
    12301 gctaacgcgc gggctaatcc cacacgttga cgcatcccgc cagagagttc atccgggtag
    12361 ctgtgggcat aattttccag cccgacctga cgcagtgcat caagggcttt ttcccggcgt
    12421 tcttcggcat taattccggc caattccata ccgaacgcag tattgtccag cacggtcata
    12481 tgcggcatta aggcaaagga ctggaagacc atcgcaatct tttttctgcg cacctcacgg
    12541 agttcggcat cggatatttt ggcaatatcc acaccatcaa tcagcacttg cccgcgggtg
    12601 ggttcaatca ggcgattgag aaggcgtacc attgtggatt tacccgagcc ggataatccc
    12661 atgatgacaa atatctcgcc ttcttcaatg gccagactgg cgtcttttac gccaagcgat
    12721 agcccagttt tttccagaat ttgttctttc gaaagtcctt gttcgatata tttgaacgct
    12781 cgctgtggat gctcgccaaa tattttataa agatttttaa tttctaattt aattgccatg
    12841 caatagaaag attcctttat ttgtttatgt cgatatatta cccaatggaa atagtcactt
    12901 ttttctaccc taacatactg agaatctgag gcaacccctg agggcaaatg tggcatgaat
    12961 attgatgcga gcaaatcgcc ggaatttccc gtgatataag ggctgagagc aaatcgagaa
    13021 aagttgatat tttttgtcat agatagcgaa aaaacgcctg aatattgtag tttcaggcga
    13081 ataatgcaac agagtatcag tgtagatcac tacagaatat ttgcgtgata aaaagctatt
    13141 tggcgggaat tatttccctg ctgcgggtag tgatattttt gaaaataaca ccctcagaaa
    13201 ttccagtctt catcttctgt ttcaaccgct ttccccatca cataagagga gcctgaaccg
    13261 gaaaagaaat cgtgattttc atcggcattc ggcgaaagcg cggcgaggat tgccggattc
    13321 acttccgcca tttctgcggg aaataacggt tcgtagccca gattcatcaa agccttattg
    13381 gcgttgtaac agagaaacgc tttcacatcg tcagcccacg gggtttcggc gtacagctca
    13441 tcggtgtatt gcaactcgtt gtcgtagagt tccagcaaca aatcgaaggc gaaactcttc
    13501 aactcttcac gttgtcccag agatatcttt tccatgtttt tctgatattt atagcctatg
    13561 tagtaaccgt ggactgcttc atcgcggata atcagacgga tcagatctgc ggtattggtc
    13621 agctttccgc ggctggaaaa atacatcggc agccagaaac cggaatagaa caaaaaagat
    13681 tcaagaaaca cactggcgat tttctttttc agcggatcat caccgcgata atgttgctga
    13741 ataatctgag cttttcgctg caacggtgcg ttttcttcac tccaggcgta ggcggcatcg
    13801 acatctttgg tctgacacag cgtcgagaaa atcgaactgt aagagcgggc atgaaccgct
    13861 tccataaagc tgatattcga taatactgcc tcttcatgag gcgtgagcgc atcgggcatc
    13921 agcgaaggcg cgccgataac attttgcagc gtgtcgagca gcgtcaggcc agtaaaaacg
    13981 cgcattgtca gttgttgttc tacgacactt aatgtctgcc atgcaggtat atcgttcgac
    14041 agtggcacct tttctggtag ccagaaattg ctggtcaggc gattccacac ctccagatct
    14101 ttatcgtcag atatcttgtt ccagttgatg gcgctgatac gtgagagttt catagatatt
    14161 ccttaaagtg cacaggagac gcagccttca atttcagtgc cttccagcgc catctgacgc
    14221 aggcgaatgt aatagagcgt tttgatacct ttgcgccagg cgtaaatctg cgctttgttg
    14281 atatcgcgag tggtggcggt atcggggaaa aacagcgtca gcgacaaacc ctgatcgaca
    14341 tggcgagtcg cttccgcgta ggtgtcgatg atcttttctg cgccgatttc gtaagcatcc
    14401 tgatacagcg ccagattctc gttagtcata aacggggcag ggtagtaaac gcgtcctgtt
    14461 ttgccctctt tgcgtatctc tactttcgcc acaatcggat gaatactcga cgtagcatgg
    14521 ttgatataag agatagaacc ggttggcggc accgcctgga gattctggtt gtatatgccg
    14581 tagcgcatca cgtcgtcgcg cagttgcgcc cacatctcac gggtaggtaa cgtaataccg
    14641 ctgcgggcaa acagttcgcc aactttcgcc gttttcggct gccagttacc ttgcagatat
    14701 tggctgaaat attcaccact ggcatagcgt gactgtttga acccggcgaa ggtttcaccg
    14761 cgttcgcgcg ccagcaacat cgaggtacgc agtgcgtgcc aggtgatggt atagaaatag
    14821 agattggtga aatccagggc ttccggcgaa ccataagcga tgccttccct cgccagataa
    14881 ccatgtaaat tcatctgccc cagtccgatg gcgtgcgagg cggcatttcc ggcttcgatg
    14941 gacggcacgc tgcggatatg actcatatct gacaccgccg ttaaaccgcg tacggcagtc
    15001 tctaccgtgc gggcaaagtt gggggaatcc atggtgtggg caatattcaa cgaacctaaa
    15061 ttgcaggaaa tatcatggcc tgtacgggca tagtcgagat tctcgtcata ctctgaggcg
    15121 ctgttaacct gcaaaatttc tgagcagaga ttactcatat ttatgcgccc ggcgataggg
    15181 ttagcacggt ttaccgtgtc ttcatacatg atgtagggat agccggactc aaactggatt
    15241 tctgccagtc gctggaagaa atcacgggcg ttgaggtatt ttttgcggat gcgttcatcg
    15301 gcaaccagtt cgtcatagtg ttcgctaatg gcgatatcgg caaacggctt gccataaact
    15361 cgctcaacgt catagggcga aaacagcgcc atctgcgcat tctcttttgc cagatggaaa
    15421 gtgatatccg ggatcaccac gccaagcgac agtgttttaa tgcggatttt ttcgtcggca
    15481 ttttcccgtt tcgtgtcgag aaaacgcaga atatcgggat gatgagcatg taaatagact
    15541 gcaccagccc cctgacgcgc gccgagttgg ttgggatagg aaaatgcgtc ttccagcatt
    15601 ttcatcaccg gaattacgcc agaagattga ttttcaatac gtttaattgg cgcgcccgct
    15661 tcccgcagat tcgacagcaa aaatgccacg ccgccgccgc gtttcgacag ttgcagtgcg
    15721 gaatttaccg cccggccaat cgactccata ttgtcttcaa tacgcagcag aaaacaggaa
    15781 accagttcgc cgcgctgctg cttaccgcag ttgaggaatg ttggcgtggc tggctgaaag
    15841 cgtcctgaca gcatttcatc ggtcagttgc aacgccagcg tcgcatcgcc ttgtgccagc
    15901 gtcagcgcca ccatcgtgac tcgatcggca aaatcttcca gataacgttt accgtcgaat
    15961 gtcttcaacg tatagctggt gtaaaacttc catgccccga ggaatgtctg gaaacgaaaa
    16021 ccgctggtgt gtgcgtgggc aaacagcgta atgacaaagt cgcgagagta gcgattaaga
    16081 acgctttcat catagtaacc ttcgttgacc agccagttca ggcgctgctg ctggctactg
    16141 aaggtcacac tgttcggacg cacatgcgtc gcaataaagg cgtcaacggc ctggcggtct
    16201 ttatcgaact gaatgcgacc tgcgctatcg tagaggttaa gcatcgcatt cagcgcgtgg
    16261 taatccatcg tttcctgcgt caggcattct gcggttgtcg ttgccaaaat tcggttactc
    16321 ctttacgaac gttttcgata tcgctttgcg tacccatgag ttcaaaacgg tacagccacg
    16381 gcacgccgca tttccgggca atcacatctc cggcgcggcc atacgcctca ccaaagttgc
    16441 gattaccaga agcaataacg ccgcgaagca acgcccggtt gtgctcgtcg tttaaaaagc
    16501 gaattacctg tcgtggcacc gcgccagccg taccgccgcc gccgtaagag ggcacgatca
    16561 ggatgtaagg ctcgtctacc tgaatccgtt cccgctcatt gagcgggatg cgcaccgcgg
    16621 gcagacctaa acgttcgata aaacgctgcg tgttttcgga gctgctggag aagtagacga
    16681 gctggctcat gcactggccg cgtgtggcgc tggatgcaga cggttaatca tgtccggacg
    16741 gaaaccagac cagctaagat cgccagcaat cactaccggc aactgacgaa agccctgagc
    16801 acgcaacgct tccgctgctt caggaacgcg atcgacatta atcatttcaa aatcaaagcc
    16861 ccggttttcc atcgcccgtt tggtggcgtg gcactgaacg caatcgttac gagtgtaaat
    16921 agtaatgcgc atggttcgta tttccgttta aaatgaagat acggcgcgat gatacgcgtc
    16981 gggttgtctc tctgttgata cagagatact agatgtagtt gaaaaaagat tcaaccacac
    17041 aatatatagc aaatagtggt cgaaattacc ctggatatga gcgtgacggg gtagggggat
    17101 ttttgtgatt caccaggcaa aaagaaaccc cgaagccagg cttcggggtc aaagacgcgt
    17161 atttattatc atttttgcac tacgatttgc gcatgcttaa cagtgcgccg ataaaaatac
    17221 ctaccgcagc tgctgtaccc acgctacacc agggtctttc acgaacaaaa gaatccgcgc
    17281 agccaacggc atcgcgtgcg gcttgctgga cgcgagtacg accatgcatt cgcgccctgg
    17341 tttctttcag caatgcctga gctttactgc gtgcggcttc agcttcccct ttggcgtcgc
    17401 tgccccagga cttcaataca gattcgaggc tgtcagctaa ttgattgaca tcattctgga
    17461 tatcctgcac gccatcatca acgtcgttgc ggttcggtcg gttaaacata tgatccccca
    17521 ttagaattcg atgtaatttt cagtgtagac cataattttc ttaactgagg ttcatcgcgg
    17581 ctttatggac ttttctgaaa gcgttgtgaa tgcgcaatca ggtaaggtag gcccgcggtt
    17641 gataataacg aggatcaaat atgtatttac gaccagacga ggtggcgcgc gtacttgaaa
    17701 aagtcggttt tactgtcgat gtggtaaccc agaaagcgta tggttaccgc cgtggggaaa
    17761 attatgtcta tgttaatcgc gaagcgcgaa tgggacgtac cgctctggta attcatccaa
    17821 cattaaaaga acgcagttca acactggctg aacccgcttc cgatattaaa acttgcgatc
    17881 attaccagca atttccgctc tatttagcag gcgagcgaca cgagcattac ggtatcccgc
    17941 atggctttag ttcgcgtgtt gcgcttgaac gttatttgaa tggtttattt ggcgaagcca
    18001 gttaaagacg cgactggcga tgccagtcgc gaaaagaaga gtcaggcttt tacctgctgg
    18061 taacggctga ctttaaacag tcggcggcaa taatcgagga agtagccata aaccgccccc
    18121 atcaacatcg aaacaacgat gtttgaactg accgccgcca taatctgatg ccagtctgcg
    18181 cccaccacta acaagatcgc cacatacacc ggtgactgga acgtcacata agccaggata
    18241 tcagccagat tttttatcca gcccgacggg ctaactttgc gtgccgcgcg cataaacaga
    18301 tcgcggtaca taccgtatgg ccatgcaatt aagatgttcg ccggaatcgc taccaatctg
    18361 gaataaaaag actgttcgaa gctcattccg gagaggaaaa cttcaataca catgttcacg
    18421 acagaacagt aaacaaccat cgcgaacgta tctgcaactg catgacgcaa gcgtgactgc
    18481 ggtgagaaca tgctaatgct ccttaataaa agcgtaatgg gatgttaatg gagatgaaag
    18541 cagtggatct cattgccatc tgcattttcg catagcgtat atcgctggct atgactattc
    18601 aattagtgaa aaatttttat ttagtcagtt tttgtagata aaatattccg taacggctcg
    18661 ttttttgtat actgtggatt tttacatgcc gatatgattt attttcaatt aaatcaattg
    18721 gatatgacct tgcctttaat ttcactaatg aaagtttggg ggtgaggtaa cgctataagc
    18781 gtagagaatt cggtgtatgt cgtgaccggg tcatttacag cattttttat gccagaaaat
    18841 gtacattaac agcagaaagc accagtgagg ggtgttttct tttattctgt tatattgcat
    18901 ttttattcac ttcggttttg tcaggcttgc ggaattagcg agcagagagc accactctg
//
LOCUS       DAZGOB010000090        18531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGOB010000090 DAZGOB010000000
VERSION     DAZGOB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.090"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(386..1387)
                     /locus_tag="ACHLIF_003378"
     CDS             complement(386..1387)
                     /locus_tag="ACHLIF_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO9607338.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1494..1790
                     /locus_tag="ACHLIF_003379"
     CDS             1494..1790
                     /locus_tag="ACHLIF_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO9607339.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1924..2349)
                     /gene="arsC"
                     /locus_tag="ACHLIF_003380"
     CDS             complement(1924..2349)
                     /gene="arsC"
                     /locus_tag="ACHLIF_003380"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO9607340.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2362..3651)
                     /gene="arsB"
                     /locus_tag="ACHLIF_003381"
     CDS             complement(2362..3651)
                     /gene="arsB"
                     /locus_tag="ACHLIF_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO9607341.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3705..4058)
                     /gene="arsR"
                     /locus_tag="ACHLIF_003382"
     CDS             complement(3705..4058)
                     /gene="arsR"
                     /locus_tag="ACHLIF_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO9607342.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4798..4881
                     /gene="dinQ"
                     /locus_tag="ACHLIF_003383"
     CDS             4798..4881
                     /gene="dinQ"
                     /locus_tag="ACHLIF_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO9607343.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4935..6287)
                     /gene="gorA"
                     /locus_tag="ACHLIF_003384"
     CDS             complement(4935..6287)
                     /gene="gorA"
                     /locus_tag="ACHLIF_003384"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO9607344.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6359..7201)
                     /gene="rlmJ"
                     /locus_tag="ACHLIF_003385"
     CDS             complement(6359..7201)
                     /gene="rlmJ"
                     /locus_tag="ACHLIF_003385"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO9607345.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7404..9446
                     /gene="prlC"
                     /locus_tag="ACHLIF_003386"
     CDS             7404..9446
                     /gene="prlC"
                     /locus_tag="ACHLIF_003386"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO9607346.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHEEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9454..10206
                     /gene="rsmJ"
                     /locus_tag="ACHLIF_003387"
     CDS             9454..10206
                     /gene="rsmJ"
                     /locus_tag="ACHLIF_003387"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO9607347.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10255..11724)
                     /gene="dtpB"
                     /locus_tag="ACHLIF_003388"
     CDS             complement(10255..11724)
                     /gene="dtpB"
                     /locus_tag="ACHLIF_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO9607348.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11890..11943
                     /locus_tag="ACHLIF_003389"
     CDS             11890..11943
                     /locus_tag="ACHLIF_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607349.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(12042..12476)
                     /gene="uspA"
                     /locus_tag="ACHLIF_003390"
     CDS             complement(12042..12476)
                     /gene="uspA"
                     /locus_tag="ACHLIF_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO9607350.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12867..13202
                     /gene="uspB"
                     /locus_tag="ACHLIF_003391"
     CDS             12867..13202
                     /gene="uspB"
                     /locus_tag="ACHLIF_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO9607351.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13447..14946)
                     /gene="pitA"
                     /locus_tag="ACHLIF_003392"
     CDS             complement(13447..14946)
                     /gene="pitA"
                     /locus_tag="ACHLIF_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO9607352.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15178..16380
                     /gene="yhiN"
                     /locus_tag="ACHLIF_003393"
     CDS             15178..16380
                     /gene="yhiN"
                     /locus_tag="ACHLIF_003393"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO9607353.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16696..17748)
                     /gene="yhiM"
                     /locus_tag="ACHLIF_003394"
     CDS             complement(16696..17748)
                     /gene="yhiM"
                     /locus_tag="ACHLIF_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO9607354.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            18131..>18531
                     /locus_tag="ACHLIF_003395"
     CDS             18131..>18531
                     /locus_tag="ACHLIF_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607355.1"
                     /translation="MKAIDNIFKYVPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNT
                     TDTFIFTIIPDNNHVLKLSSPITVTVECKGG"
BASE COUNT         4888 a         4584 c         4684 g         4375 t
ORIGIN      
        1 acgttaacaa ctatcaaaaa gcgctcattt taaaaagtta ttttgttctc cgatagtgtg
       61 tgaagtaata tttttgtgat gttttagatg tgtaataatg aaccaggtta aagtgtattt
      121 aaaaatgtgt tgtattagcg actgaaatct gccttttaaa aaggaggagg gtactgagac
      181 aaagagtcat tgcttctccg ataaaatgtc agagaagcat gattacgtgg ttattgtctg
      241 ttgatgactt gttgaccgag ccgtcatcat tctcaatgca ttagttaaat catttgtgat
      301 ttttaaggat tatcctgtct atgattttct acttagaagg atatggcact aactatgcat
      361 cttatagctt actgctggca tctatttaaa atagcaattg agtcaaatag cccatcaaga
      421 tagccatacc taatataacc gtcaggaatg cagctatcat cggcattctg aacattgatt
      481 taagtaatat cacttccgtc aggcttgcgc cggcactgcc gatgattaat gccattaatg
      541 ctcccagacc cattcctttt gtcatcagaa cagatgccag agggataact gcctcagccc
      601 ggatatacag cggaataccg acaacggcgc ttaatgggat ggcgaaggga ttatctgctc
      661 ctgcatgagc ggcaatccac gctgagggaa taaagccata aataaaagat cctattaata
      721 ctcctaacaa aagatatggc agtacatctt taaactgttg caacgcatct ttggtggcta
      781 gttttatggg attaatactt actacagctt tggtactgca acaggtaact acggggttct
      841 caatggcttt agccgctgga ctacaacaac tcacttttat gggcgtatat gtcgttcccg
      901 gcgaagtttt tgctggagca caacaatttg ctgatgagct tttactggca atgatatgac
      961 gttcaaaccc cagagaatcc aggataatac tggcaagaac ggagacgccg gctgcgataa
     1021 tcgcgtacaa caaggtgact ttccaaccaa aggtcatcca cattagtccg acgataatgg
     1081 gattaagtaa tggtgaaaca aataaaaaag tgagagtcgg accaaacccg gctttcgctg
     1141 acaacaatcc acgtaacatg ggtattgtcg agcaactaca gaacggggta acggctccca
     1201 acagagcagc caggagatag cctctccctt ttctggcccc catcatctgc tggattttat
     1261 ggtctggcac cttttgtctt atcaggctga caccggcact aatcactata aacagcaacg
     1321 aaagctcgac ggcgagaaac acaaacatct ctgcggcatc ttgcagcatc gcaagccaac
     1381 tactcatacg taacctcatt ggataaatct atatttccag aataatcgaa atgaaattca
     1441 gatcaagata tttctggtat tatcgaaata tcaaccagac agaggggtgt catatgcagt
     1501 tggaagaagt cgctaaagcg ctgaaagagc taggtcaccc gacccgtttg ttcatattta
     1561 aacatcttgt caaagccggg gaacaagggt tgccggtcgg tgagttgcaa aagcagcttg
     1621 ggatacctgg ctccacactc agccaccata tatcagcact ggtatcggta ggactggtca
     1681 aacaaaaccg tgagagtcgg acgctgatgt gtgtttcgca atacgcaatg ctggaagcga
     1741 taattgaatt cttacgcgag gagtgctgcg taaatagcaa aattgcctga tgcgctacgc
     1801 tctacatatt tcatgccata tattgaattt gcacggtttt gtaggtcgga taagacgcgt
     1861 tagcgtcgca tccgacataa acaaagcgca ctttaccaac tatcggcagt ggatatcgcc
     1921 gctttatttc aggcgcttac ccgcttcatc aaccactttc tcgccatctt ccttggagaa
     1981 tgcgcctttt tgcgcatctg gcagaatttc cagcaccact tctgaagggc ggcacaggcg
     2041 agttcccagc ggcgtcacca caatcgggcg attaatcaga atcgggtgct gaagcataaa
     2101 gtcgattaac cgatcgtcag taaatttatc ttccgcaagg cccagctcct catacggttc
     2161 gacgttttta cgcagcagcg cgcgtacgga aatccccata tcggcaatga gtttgaccag
     2221 ttcatcgcgc gttggcggag tttccagata atggataatg gtcggttctg tgccgctgtt
     2281 gcggatcatc tccagcgtat tacgcgacgt gccgcaggcc gggttgtgat aaatggtaat
     2341 gttgctcata tcagtatctc attacaaagt gaaagagaga cgtagcgcca gcgcagccag
     2401 cgtcacaaac agcacaggca gggtcatgat aatccctgta cggaagtaat atccccagct
     2461 gatagtcata ttcttctgcg aaagtacgtg cagccagagt agcgtagcca ggctaccaat
     2521 tggggtaatt ttcggtccca aatcgcagcc aatcacattg gcataaacca tcgcttcttt
     2581 gataacgcca gatgccgtgc tgccatcaat ggacaacgcg ccaaccagta ccgtcggcat
     2641 attgttcata atagaagaga ggaaggcggt gaggaatccg gtgccgagcg tcgcggccca
     2701 caggccgtta tccgccagca cgttgagtac gccagaaaga tattccgtta atccggcatt
     2761 gcgcaggcca taaaccacca gatacatgcc gagcgagaag atgacaatct gccagggggc
     2821 accgcgcagg actttacccg tattaatcgc atgaccgcgt ttagcgacga caaataatat
     2881 cagcgcgccc acagctgcaa tggcgctcac cggaatgccg agcggttcca ggacgaaaaa
     2941 tcccaccagc agaagcagta aaacaaccca gccagttttg aacgtagcag gatctttgat
     3001 cgcttctgcg ggagatttca gcagcgccat atcgtagttc tgcggaatat ctttgcgaaa
     3061 atagagatgt aacatcacca gcgtggcaac aatcgcggcg atatccaccg gcaccatcac
     3121 cgaggcgtat tcgcgaaagc cgaggccaaa gaaatcagcg gaaacgatat tcaccaggtt
     3181 ggagacaata agcggcaggc tggcggtatc ggcaatgaat ccggccgcca tcacgaacgc
     3241 cagcgtagtg cctttactga accctaaagc cagcagcatg gcgatgacaa tcggtgtcaa
     3301 aataagcgcc gcgccatcat tggcaaacag ggcggcaacg gcagcaccga gcaggacaat
     3361 ccaggtaaac agcaagcgac cacgaccatt accccagcgt gagacgtgca gcgccgccca
     3421 ttcaaaaaag ccggactcat ccagcagcag gctgatgata atgacggcga taaacgcagc
     3481 cgtcgcgttc cagacgatat tccacaccac cggaatatca cccggatgga ccacgcccgt
     3541 aactaacgcc agtactgcgc cgagcgttgc actccagccg atgcctaaac ctttcggctg
     3601 ccagataacc aatacgatgg tcaggacaaa gatagcgcct gccagtaaca taatgcctcc
     3661 cggataaaac acatctgaaa attcatatgt gtttagctaa atttttaact gcaaatgttc
     3721 ttactgtccc cggaacagtt ttgtcgagcc aggttgcgga caatcgcctg aaccttttcc
     3781 tgttcacatc gccaggcctc atcaataatt ttcgccgccc atgctggaat atgcggtgat
     3841 aagcggtaat gaacccactt accttgcttg cggtccagca atagcccgct ttcacgcagc
     3901 aatgccaggt ggcgggagat cttgggctgc gactggtcga gagcagtgca gagatcgcag
     3961 acgcataact ctcccagttc gctgagcagt aaaacgatgc ccagacgggt ttcatcagca
     4021 agaattttga acaattggat gggtaacaga aatgacatat tgcgctcctg attgttgcag
     4081 gtagtgtctc tcttcgaagc ggataagtca aaaacatata tgacttaacg aatgtgtaag
     4141 tgcagaggaa ggtaataggt gtgaattttg agttggctat tcatttgaaa ggaggtctga
     4201 atcaggacgt aaaaaaaccg actttgcgtc ggttttttac tttccagccc tgagttggtg
     4261 gctctggctg gagtgagaga gttcttatct aacagctcaa tactattagc cagtggctaa
     4321 cattgcaaga attagcgatt tcttcagctg gcggaggggg cacttcggta tatatcgcaa
     4381 tgacgattta tatactttca ctgggtcatc gtcatattaa gccttgtccg aatccggatg
     4441 taaaaaaacc gactttgcgt cggtttttta ctttccagcc ctgagttggt ggcactggct
     4501 ggagtgagaa tgccattatc taacgcatca atgctaatac catactgaaa ctattgcaag
     4561 gacatgctgg ttttataacc tgaatgtact gtatgattat ccagttagct ctgaggcatt
     4621 ttcactctgg taatgcgcat aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc
     4681 gttaactgat gctctggccg gagtgagaat gccattatct aacaataggg catgcgccgt
     4741 gacaggcagt ggatgagtaa gcggatgcat tctcactcca tcgcatggag aaaacgggtg
     4801 attgataaag caatcatcgt tcttggggcg ttaattgcgc tgctggaact gatccgcttt
     4861 ctgcttcagc ttctgaactg atagcggaaa cgtaattaag ggctaagagc acactactct
     4921 tagcccttta acatttaacg cattgtcacg aactcttctg ccgccgttgg gtgaatggcg
     4981 acggtattgt cgaagtcttt tttggttgca cccatcttca gcgccaccgc gaagccctgc
     5041 aacatttcgt ccataccaaa gccaatgccg tgaataccga caatcttctc ttccgatcca
     5101 acgcacacca gcttcatgcg gcacggctgg cggtgagtgg tgacggcggt atacatcgcg
     5161 gtgaaagagg atttatacac tttcacctga tcgtcgccat actgctcgcg cgcctgcggt
     5221 tccgttaaac caacagtacc aatcggcgga tggctgaaga ccacggtcgg aatgttgctg
     5281 taatccagat gctcatccgg cttgttatta aacaggcgtt cagagagacg gcgacccgct
     5341 gcaactgcca ccggtgtcag ctccactgca cccgtgttat cgcccaccgc gtaaatacct
     5401 tcaatattgg tgttttgata tttatcgacg acgatatagc ctttttcgtt agttttaacg
     5461 ccagcggctt ccaggttgat gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg
     5521 caatccaccg tttcactgcg accatcttcc agctccagcg tcaggctacc atcggcattt
     5581 ttcactaccg ctttcgggat ggcgttggtg tgcagctgcg ggccttcggc gttcatcact
     5641 tcgaccagcg tttcggaaat catcgggtcg aagctgcgca gcggcgcatg tttacgcaca
     5701 aacagatgcg ttttcgcgcc gaggccgtta atcacgcccg ccagctcaac ggcgatgtaa
     5761 cccgcgccaa caaccgccac gcgctctggc aaagcaggaa gggcgaagaa gccatcagaa
     5821 tcaataccgt attccacgcc cggaatatcc gggtggctcg gacgaccgcc tgtggcgatc
     5881 agaatatgat cggccgtgat ggtttcgccg tttacctcca gcgttttggc atcaacgaag
     5941 cgggcaaagc ctttgattac atcaacgtta tttttaccga gcacgttttc ataggaagta
     6001 tgaatacggt cgatataggc ggtacggctg gcgatcaacg tttcccagtt gaatttattg
     6061 atagtggtat caaaaccata atccgggccg tacatatgga tcgcttcacg gatttgcgcc
     6121 gcgtgccaca tcactttttt cggcacacag ccaacattta cgcaggtgcc gcccagctct
     6181 ttggcttcaa tcagcgcaca tttctggccg tacatagccg cgcggttgat ggaggcgata
     6241 ccgccgctgc cgccgccgat ggcgatgtaa tcatagtgtt tagtcatgac aaagtgtcct
     6301 tatcgttgat taccgcgatt gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt
     6361 actccggcac gatccagctt acggtggcgt gcccggtgcc tgccggaacc agtttgctgt
     6421 gcagccacgg cagcacgtta ttcatctgct gttccagttt ccacggcggg ttaatcacaa
     6481 tcatgccgga agcggtcatg ccacggcgat cgctgtctgg cagtaccgcc agttcaattt
     6541 gcagaatttt gcgaataccg gtcgcttcca gatcgtggat catgcgctta atttgctgac
     6601 gcagcaccac cggataccac agtgcgtaag taccagtggc gaaacgtttg taaccttctg
     6661 ctatcccgct gaccaccgcc tgatagtcgg ttttcatttc atacggcggg tcgataagga
     6721 ttaaaccacg gcgggaaacc ggcggcagtt tggccttaag ctgctggaaa ccatcggctt
     6781 tttcgacgcg cgcacggctg tctttctgaa attcagaacg cagcaacggg taatcgctcg
     6841 ggtgcagttc ggtcagttgc aggctgtctt gttcgcgcag tagctggcga gcaatcaatg
     6901 gcgaaccggg gtagtaacgc aactgaccgc tacggttgaa gtgttttacc acattgatgt
     6961 acgcctccag ttctgcgggc aaatcgtcct gctgccagat acgggcgatg ccttcgagat
     7021 attcgccggt acgctcggca tgttcgctgc ctaactgata acgcccggcc cctgcgtggg
     7081 tgtcgagata gagaaacggt ttatctttct ctttcagcga ctcgatgatc aggctctgaa
     7141 cggtatgttt aaggacgtcg gcgtggttgc cagcgtgaaa gctgtggcga taactgagca
     7201 tgggtaaagg tgttccggta agtaaaaatc ggcccgtatt cggggcgcac aaaagcgtat
     7261 caggacagta taccgaaaag aggccgccgc cgcgaaagcg taacgtttct cattgaaatt
     7321 cactacactt aaccccatgc tacacacatt atgtaaagcg cctgttgagc gcttccttaa
     7381 cctctttaac caggactgcg cgaatgacga atccgttact gactcccttt gaattgcctc
     7441 cgttttctaa aattctcccg gaacatgtcg ttccagccgt gactaaggcg ctgaacgact
     7501 gccgcgaaaa tgtggagcgc gtagtagcgc aaggagcacc gtacacctgg gaaaatctct
     7561 gccagccgtt ggcggaagtg gacgatgtgc tggggcgtat cttctccccg gtcagccacc
     7621 tgaactcggt gaaaaatagc ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc
     7681 tgtcggaata cagcacctgg gtagggcaac atgaagggct gtataaagcg tatcgcgacc
     7741 tgcgcgatgg cgatcattac gccacgctga acacggctca gaaaaaagcg gttgataacg
     7801 cactgcgcga cttcgaactc tctggcatcg gtctgccaat agagaaacaa caacgctacg
     7861 gtgagattgc cactcgtctt tccgagctgg gcaaccagta cagcaacaac gtcctcgatg
     7921 cgacgatggg ctggaccaaa ctcgttaccg acgaagcgga gctggcgggg atgcctgaaa
     7981 gcgcgctggc tgcggcaaaa gcccaggccg aagcgaaaga gctggaaggc tacctgctga
     8041 cgctggatat cccaagctac ctgccggtaa tgacctactg cgacaaccag gccttgcgtg
     8101 aagagatgta tcgcgcttac agcacccgcg cctccgatca aggcccgaac gccggtaaat
     8161 gggataacag caaggtgatg gaagagatcc tcgctctgcg tcacgaactg gcgcaactgc
     8221 tgggctttga aaactacgcc tttaaatccc ttgccactaa aatggcagaa aacccgcagc
     8281 aggtgctgga tttcttaacc gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc
     8341 tggcacaact gcgcgccttc gccaaagcgg aatttggcgt cgatgagttg cagccgtggg
     8401 atatcgcgta ctacagcgaa aaacaaaaac agcacctcta cagcatcagc gacgagcagc
     8461 tgcgtccgta cttcccagaa aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta
     8521 tttacggcat caccgctaaa gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt
     8581 tcttcgaact gtatgacgaa aataacgaac tgcgcggcag cttctacctc gatctgtatg
     8641 cccgtgaaaa caagcgcggc ggggcgtgga tggatgactg cgtaggccag atgcgtaaag
     8701 ctgatggttc gctgcaaaaa ccggtcgcgt atttgacttg taacttcaac cgcccggtaa
     8761 atggtaaacc ggcgctgttc actcacgagg aagtgatcac cctgttccac gagttcggtc
     8821 acggcctgca ccatatgctg acccgcatcg aaaccgctgg tgtttccggt atcagcggtg
     8881 tgccgtggga tgcggtcgaa ctgccgagtc agtttatgga aaactggtgc tgggagccgg
     8941 aggcgctggc gtttatctcc ggtcactatg aaaccggcga accgctgccg aaagagttgc
     9001 tggataaaat gctggcggcg aagaactacc aggcggcgct gtttattctg cgtcagctgg
     9061 agttcggcct gtttgatttc cgccttcatg ccgagttccg cccggatcag ggggcaaaaa
     9121 tcctcgaaac tctggcagaa atcaagaaac tggttgccgt ggtgccatct ccgtcctggg
     9181 gccgtttccc gcacgctttc agccatattt tcgccggtgg ttatgcggca ggttactaca
     9241 gctacctgtg ggctgacgta ctggcggcag atgctttctc gcgctttgag gaagagggca
     9301 ttttcaaccg tgaaaccggg cagtcgttcc tcgacaacat tctgagccgt ggcggttcag
     9361 aagagccgat ggatctgttc aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc
     9421 tggagcatta cggcattaag ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca
     9481 ggcgccggag acggcgcctt atctgttctg gcggcccgct gggggctgga gcacgatgaa
     9541 gacaacctga tggcgctggt gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag
     9601 ccaaaacttg gcggcatatt tgttgatttt gttggcggag cgatggcgca ccgacgcaaa
     9661 ttcggcggtg gtcgcggtga ggcggtggcg aaagcggtgg gcattaaagg cgattatttg
     9721 ccggatgtgg tggacgccac cgcagggctg gggcgcgatg cctttgtgct ggcttcagtc
     9781 ggttgccgcg tgcggatgct ggagcgtaat ccagtcgttg ccgcattgct cgacgacggc
     9841 ctggcgcgtg gttatgcgga tgcggaaatc ggcggctggt tgcaggagcg tttgcagtta
     9901 attcacgcct ccagcctgac ggcgctaacc gatattaccc cacgcccgca ggtggtctat
     9961 ctcgacccga tgttcccgca taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc
    10021 tttcagtcgc tggtggggcc ggatcttgat gccgatggat tgctggagcc tgcacgcttg
    10081 ctggcgacca aacgcgtggt agtaaagcgc ccggactacg cgccaccgct agcgaatgtc
    10141 gccacgccaa acgcggttgt gactaaaggg catcgctttg atatctatgc aggtacgccc
    10201 gtgtaaaagt aaagcagggt agcgataacg ctaccctgct ttctgcaaga ataattaatg
    10261 gctttccggc gtcgcaatca tgcgtttcag ccacggcacc atcaacagca tcactactgc
    10321 aacgcccagc gtgaccagac caatcttacc aaacacgttg gtatagacgg gcaacgtctc
    10381 aagcggatcg gtaatgttgt ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa
    10441 gaacgcggca gcctgcgtca ggaaccacat cccgagaata aagcccatca aatgctgcgg
    10501 caccagggca gcaatcatcg ccaggccaag ggcgctaata aacagttcac ctaagctctg
    10561 gaataagtac accagcacga taaaccatgg cgatgtcagc ccttgtgcat ccgcaaacca
    10621 cattcccgca gctgccgccg tcaaaaagcc cagtgagcac ataaacatgc cgagagtaaa
    10681 tttcatcggc atcgagaggt ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag
    10741 tattgggctg gcgagtacca cccagaacgg gttaagcgcc tggaagctga ccgggttgat
    10801 ggaaaaaccg agaatttcat gatgcacgtt gttgatggca aagaagttca gcgatgttgg
    10861 catctgggcg tagagaatgt aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa
    10921 cattttattg cgcccggttt tatccagctt gaatgcctga cgaaagaaga tgatggtgac
    10981 gacgatggag agaacaatca gcaccagatt ggcgacttct acgttgtgca tcagccatgc
    11041 acatacgaag atcatcacca cgctgccaag taatacgtac aacagtttgc tgaagctcat
    11101 cggcttgaag tcgggttcag aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac
    11161 cagtaatgcg ataattaacc ccgccccgca caggttgtag gtgactgaat aaccgaatct
    11221 atcagcgatc acaggggcca gcgataacgc tatcaacgag ccgatattga tcgacatata
    11281 gaacagggtg aatgcgccat caagccgcgg atctttcggc ggatagcact tcgaaagcaa
    11341 gctggctggg ttagctttaa acaggccgtt accgacagcg atagtcccca gggcgatgaa
    11401 aatcaggtca ggcttaagta gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag
    11461 tgctccgaga acaatggtgc gtttggtccc cagcaggtgg tcgccgacat agccgccaat
    11521 ggaaatgagg ccatagacca gcgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg
    11581 cgagaatcca agctgtttaa cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc
    11641 gaatcgctcc cataactcga caaaaaagat catgaaaaat gggcgaggtt gctgcagcat
    11701 ccccatgggt gttgttgtat tcatatttat taaccttcca ataccatcct gaaaagtatg
    11761 aacgcgtaag ggataacgcg cgaaaaagtt tacatctgtc agagattctt aatattgatg
    11821 cggtgaatag attaatacac tattgcaaca gaaaataacc agctgatcac actaatgatg
    11881 gaaaatatga tggtgttaaa tgttgtattg gcaggagatt ttgattgcgt acgcagatcg
    11941 taggccagat aaggggttta cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc
    12001 ccgcatatgc gggcgttcag gaacacgtcg tggagggatg attattcttc ttcgtcgcgc
    12061 agcggaacaa tcagcatatc aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc
    12121 agtttgctcc agaagtcctg gtggtgacca caaaccacca gatccatatc gtatttcttg
    12181 attgcatcga ccagaacctg gcccaggtcg ccgctgccgc tcagggtttc agtgattggg
    12241 tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc
    12301 tgcatatcac ccagattcac atcaataagc ccggtgtata ggtcagagta gtttacatct
    12361 acgtggatca gagaaacttt cgcattgtag gggcgagcca tagagactgc tttctctacc
    12421 agaactttgc tttccgggga gagatcgact gcgatgagaa tgtgtttata agccatagtg
    12481 ttactccttc cataaagttg tcgatgactg gccagctagc gtttcttgtg ccgttacgtc
    12541 acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag
    12601 accatcctta ccttatagcg acccggatga tccgtcaatc cgccttgctt acccgttaga
    12661 taaacaaatt ttcgacatga atatattgat agtggttaac cttctggaaa aaaaacaacc
    12721 tgatctccta cactatctat agagccgctc ggatgttggt caccacaaaa gcggtttctc
    12781 caggacctgg tgttaactga ttggcagcgt atcgggagcg gtagccccgg ggtggaactc
    12841 cgcgggcagg tcgccgggga ggaggtatga taagcaccgt cgcattattt tgggctttat
    12901 gtgtcgtttg cattgttaac atggcgcgct atttctcatc actacgcgcg ttgttagtgg
    12961 tactgcgtaa ctgcgatcca ttgctctatc aatatgttga tggagggggc ttttttacct
    13021 cacatggcca acccaacaaa caggtgcgtc tcgtttggta tatctatgcc caacgttatc
    13081 gcgatcatca cgatgatgaa tttattcgcc gctgtgagcg ggtgcgtcgg cagtttattc
    13141 tgaccagcgc attgtgtggt ctggtggtgg tcagcctgat tgcattgatg atttggcatt
    13201 aagcacttca gcaataataa cgcataaaaa aagcgggcca gtaaactgac ccgctcgaga
    13261 ctgattacaa acgcaaaatt gcctgatgcg ctacgcttat caggatctac ataatctctg
    13321 caatctattg aatttgcatg tttttgtagg ccggataagg cgttcacgcc gcatccggca
    13381 tgaacaaagc gcactatgtc acaatctgaa gcgggccagc tgacccgctc gttttggtgt
    13441 gtacgattac aggaactgca aggagagcca gtacagtccg ccggaaagca gtaccgcagc
    13501 cggaagggta aacacccagg ccatcagaat actggttacg gttttacgct gtaagccgcc
    13561 gccatctacc accatcgtcc ccgcgacaga agaggagagt acgtgagtag tggaaaccgg
    13621 catcccggta taactcgcca ggccgataga cactgccgcc gtcatctggg ccgacatccc
    13681 ctgagcgtag gtcatgcctt tcttaccgat tttctcaccg atagtcgttg ccacacggcg
    13741 ccagccaatc atcgtaccga tacctaacgc cagcgcgacc gccatgatga tccacaccgg
    13801 tgcatactcg atggtgctaa gcatgtcgga cttcagtttc ttcaacaggc gctgatcgtc
    13861 agcactcaca ccaggcatct tcaccacttt gtcgatagtg tcagaaacgc acagcataat
    13921 gcggcgcatc tggctacgtt gatcaagcga cagcttgtcg tagctttcca catcggtagt
    13981 caacatgcct ttcaggcggt tgagcgcgtt aatggtattc gacggatggc aatggaactc
    14041 cgcgggttgc gttgcgccag cttccggagc cggtactaac tgatcagcac cggtagcctg
    14101 tttgagcagc gcaggatgct gctcaaagta agcttcgacg ttgttgatgg catcacgggt
    14161 acgggtgatt tcgtagccag tggcattcat gttcaccacg aagcctgctg gtgcgacgcc
    14221 aatcaatacc aacataacca gaccaatgcc tttctgacca tcgttcgcgc cgtgcgaaaa
    14281 cgccacgccg atagcggaaa ggatcagcgc aatacgcgtc cagaacggcg gctttttctt
    14341 gccgtctttc ttttcacgct ccgctggggt caggtggata cgggcgcgtt tcttggtgcc
    14401 gctccagtag cgacgcagca agaaaatcag accgccagca aacaccaggc cgacaatagg
    14461 ggaaacgatc agagaaccga aaatacttaa tactttcggg atattgagtg catccaccac
    14521 tgacgtcccg gtcatcaacg cattggttaa accaatcccg atgatcgcgc caatcagcgt
    14581 atgagagctg gatgcaggta aaccaaagta ccaggtaccc aggttccaga taatcgccgc
    14641 cagcaacata gagaacacca tggcaaggcc atgagacgat cccatattaa gcagcagatc
    14701 cgtcggcagc atatgcacaa tggcataggc aacactcaga ccacccagca aaacacccaa
    14761 aaagttgaac accgctgcca taaccacggc gagctgagaa cgcatcgcgc gggtatagat
    14821 aacggttgcc acggcgttgg ctgtgtcatg gaaaccattg atggcttcgt agaacagcac
    14881 aaaagccagt gcaagcaata ataacagccc ggtatgcaaa tccaggccag caaacaaatg
    14941 tagcatagga cgttacgcca ttttgaggac atgaacgcgg cgcattatca gtgactttcg
    15001 cggcgcgggc aaagtgaaat atagactttt tttgatttgc ctcctgtatg gatttcactc
    15061 aaaaaataat tatcttatat aattcaggca aatacttcct tttagtaata ttgatgctgg
    15121 tgcgaccact gaggaatctt tacaattcac gcccgttttt tctaagagga gcgcaacgtg
    15181 gaaaggtttg atgccattat tataggcgct ggtgcggcgg gtatgttctg ttctgcgctg
    15241 gcaggtcagg caggacgccg ggttctgctg atcgataatg gtaaaaaacc agggcgcaaa
    15301 atccttatgt ctggcggtgg gcgctgcaac tttaccaacc tttatgtcga accaggcgct
    15361 tatctgagcc agaatccgca tttttgtaag tctgcactcg cgcgttttac ccagtgggat
    15421 ttcattgatc tggtcaataa acacggcatc gcctggcacg agaaaacgtt aggacaactc
    15481 ttctgcgatg actccgcgca gcagattgtc gacatgctgg tggatgagtg cgagaagggc
    15541 aatgtaacct tcagattgcg tagcgaagtg ctgagtgtgg cgaaggatga aacaggcttc
    15601 acgcttgaac tgaacggcat gactgtcggt tgcgaaaagc tggtcatcgc gaccggtggg
    15661 ctgtcaatgc cggggctggg cgcgtcgccg tttggttata agattgccga acaatttggc
    15721 ctcaacgtgc tgccgacccg cgcgggtctg gtgccattca ctctgcataa accgttgctc
    15781 gaagagttac aggtgctggc gggcgtggcg gtgccttccg tgattaccgc tgaaaacggc
    15841 atcgttttcc gtgagaactt actcttcacc catcgcggct tgtctggacc ggcggtgttg
    15901 cagatttcca gctactggca accgggtgaa tttgtcagta tcaatctgct accggatgtg
    15961 gatctcgaaa ccttcctgaa tgagcagcgt aacgcacatc cgaatcagag cctgaaaaac
    16021 acactggcgg ttcatctacc gaagcggttg gttgaacgct tacagcaact cgggcaaatc
    16081 ccggatgttt cgctaaaaca gctcaacgtg cgtgaccaac aggcactgat tagcacattg
    16141 accgactggc gcgtacaacc caacggcact gaaggctatc gcactgccga agtgacgctc
    16201 ggcggcgtgg acaccaacga actctcttca cggacgatgg aagcgcgcaa agtgcctggg
    16261 ctgtacttca tcggcgaagt gatggacgtt accggctggc tggggggcta taacttccag
    16321 tgggcgtggt cgagtgcgtg ggcttgtgcg caggatttga ttgcagcgaa gtcgtcctga
    16381 cctgttacct aaagatgaaa attattagag ccgcattaaa aatgagctaa ttttgatagt
    16441 ggctatcttg tgattatttt ctaatgagcc cgtgaactga aaccctccag gcttaatata
    16501 aggtggaagg aaaggtgatt gaaaactcac ccagtggcca gatctttata aaatatgaac
    16561 aggttgaaaa aaacagtaac tttgttgttt ttttatcctt aaccactatg cattaatgct
    16621 gcgttatttc atgatgccta agaaaaacca gcgttacgca aatggtcaac gctggtttta
    16681 tccggtacgt tgcaattatt ttttagcaga acctgcttct aatattgaaa cgattgagaa
    16741 caacgtaaag cataccgctc ctaaaccgac caaaacccgc gaaggaataa aatatcccat
    16801 gtcggtctgc gccatttccg caagaaacga tgccaggaaa aggcaaaaca ggcaggtgaa
    16861 gacgggaatc atcggtattc tgttggctaa cgaacaggta cgtctccata ccagtgccag
    16921 tagccagact tttgagaata tgctgtaaca gatcattccg aggcaaataa gaataatgcc
    16981 gggagccagt cgggcgcttg catcggaact gactaagacg tatatcccct gcagtaccgt
    17041 gattgagccg agaaaaataa cccaataaca ccacagccag tgttctttag ttgaaaacgt
    17101 attacgtgtt tgatggacaa ttgtggcaac aaggccaatt agacaggcac agattgcggt
    17161 taacccaagc aatacgtgac ccgcgacata atgcggagtt atgtcagcac tacgtaacag
    17221 cgtaatagac caaatgaaac cgagcagggt gagtaaaacg ggaacggcaa tgaggcaatt
    17281 accgattaat gaagaataag cctgtaccgg cgttccatcg ctcttgctcc ccgctgcatt
    17341 tttgggaatg agcagaaagt gaccggatga cgctgccacc gtcgatacac aggcggcaat
    17401 cataccaacg ccgaaaataa catggccggc gacaaactcg tctgccatca catcattgcc
    17461 tgctaacagt gcccaacccc atatcatggt gataattgac cccgcatagc caataatggg
    17521 gaacaatgta ttgtaaaacg tattaacgcc gcgcgtgagc tgcgaaatga taataaatgc
    17581 ggtagtgaat aatgccaggc atatggctgc cagagaaatc agcacatgac ccgcaacgaa
    17641 atactcactt tgcccagaac tttccagcac aaagccacct aacgcgatac aaattaagcc
    17701 cataattaaa gggattaatt tgaaaagcca cccgatatat atgttcactt taaactcctc
    17761 aggtatccct gctaatattg aaatgtgcat taatgataat ttcagcctgt taatataatg
    17821 acgagatatc attaaagaca aatgcatagc ataactatag cgggatttac tttgcagagc
    17881 gctacttttc agcgttcaaa aggccatggt aaataaatat ttatcctaag attaattctt
    17941 attctgttgt tttttacaat tatcttccag ggagggattc tttttcatta tttggtaaaa
    18001 gaccggatgg tatcggtgct taaaatcagc gaacatataa gtattattgt tttatatcgt
    18061 cgaaaatatg tctttcagac atgtattatt tattttgtta ctaacaagtc aaaaatatcg
    18121 caggttattt atgaaagcaa ttgacaatat atttaagtac gtcccttcaa accataaaga
    18181 taagcattca gataaagtta ataatcatca acaccatagc aaggttgata aaacacatcg
    18241 ttcaaagata gttgaaattg ataaactgga taatgattcg caaatagata acgattttgg
    18301 catgcatatc atctatttcc tgcaacatgg tcactggagg gtaaatgatc gtagtcatca
    18361 gatggagaaa gtatggtttt acaatagtga accttctata gatattcaag aatataatag
    18421 attcgccgac aatactactg acacgtttat ctttacaatt ataccagaca ataaccatgt
    18481 gttaaaatta tcgtctccca ttaccgttac tgttgagtgt aagggcggtt a
//
LOCUS       DAZGOB010000091        18340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGOB010000091 DAZGOB010000000
VERSION     DAZGOB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.091"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(326..1354)
                     /gene="epmB"
                     /locus_tag="ACHLIF_003396"
     CDS             complement(326..1354)
                     /gene="epmB"
                     /locus_tag="ACHLIF_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO9607356.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1396..1962
                     /gene="efp"
                     /locus_tag="ACHLIF_003397"
     CDS             1396..1962
                     /gene="efp"
                     /locus_tag="ACHLIF_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO9607357.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            2014..2139
                     /gene="ecnA"
                     /locus_tag="ACHLIF_003398"
     CDS             2014..2139
                     /gene="ecnA"
                     /locus_tag="ACHLIF_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO9607358.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2250..2396
                     /gene="ecnB"
                     /locus_tag="ACHLIF_003399"
     CDS             2250..2396
                     /gene="ecnB"
                     /locus_tag="ACHLIF_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO9607359.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2578..2895
                     /gene="sugE"
                     /locus_tag="ACHLIF_003400"
     CDS             2578..2895
                     /gene="sugE"
                     /locus_tag="ACHLIF_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO9607360.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2892..3425)
                     /gene="blc"
                     /locus_tag="ACHLIF_003401"
     CDS             complement(2892..3425)
                     /gene="blc"
                     /locus_tag="ACHLIF_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO9607361.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3514..4647)
                     /locus_tag="ACHLIF_003402"
     CDS             complement(3514..4647)
                     /locus_tag="ACHLIF_003402"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO9607362.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(4710..5069)
                     /gene="frdD"
                     /locus_tag="ACHLIF_003403"
     CDS             complement(4710..5069)
                     /gene="frdD"
                     /locus_tag="ACHLIF_003403"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO9607363.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(5080..5475)
                     /gene="frdC"
                     /locus_tag="ACHLIF_003404"
     CDS             complement(5080..5475)
                     /gene="frdC"
                     /locus_tag="ACHLIF_003404"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO9607364.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5486..6220)
                     /gene="frdB"
                     /locus_tag="ACHLIF_003405"
     CDS             complement(5486..6220)
                     /gene="frdB"
                     /locus_tag="ACHLIF_003405"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO9607365.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6213..8021)
                     /gene="frdA"
                     /locus_tag="ACHLIF_003406"
     CDS             complement(6213..8021)
                     /gene="frdA"
                     /locus_tag="ACHLIF_003406"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO9607366.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8346..9323
                     /gene="epmA"
                     /locus_tag="ACHLIF_003407"
     CDS             8346..9323
                     /gene="epmA"
                     /locus_tag="ACHLIF_003407"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO9607367.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9542..11044
                     /gene="yjeM"
                     /locus_tag="ACHLIF_003408"
     CDS             9542..11044
                     /gene="yjeM"
                     /locus_tag="ACHLIF_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO9607368.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            11096..11410
                     /gene="yjeN"
                     /locus_tag="ACHLIF_003409"
     CDS             11096..11410
                     /gene="yjeN"
                     /locus_tag="ACHLIF_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO9607369.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            11407..11721
                     /locus_tag="ACHLIF_003410"
     CDS             11407..11721
                     /locus_tag="ACHLIF_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO9607370.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(11750..15073)
                     /gene="mscM"
                     /locus_tag="ACHLIF_003411"
     CDS             complement(11750..15073)
                     /gene="mscM"
                     /locus_tag="ACHLIF_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO9607371.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(15095..16063)
                     /gene="asd"
                     /locus_tag="ACHLIF_003412"
     CDS             complement(15095..16063)
                     /gene="asd"
                     /locus_tag="ACHLIF_003412"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO9607372.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(16160..17212)
                     /gene="rsgA"
                     /locus_tag="ACHLIF_003413"
     CDS             complement(16160..17212)
                     /gene="rsgA"
                     /locus_tag="ACHLIF_003413"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO9607373.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            17307..17852
                     /gene="orn"
                     /locus_tag="ACHLIF_003414"
     CDS             17307..17852
                     /gene="orn"
                     /locus_tag="ACHLIF_003414"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO9607374.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18063..18138
                     /locus_tag="ACHLIF_003415"
     tRNA            18063..18138
                     /locus_tag="ACHLIF_003415"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18096..18098,aa:Gly,seq:gcc)
BASE COUNT         4231 a         4704 c         4849 g         4556 t
ORIGIN      
        1 gcttggtagt aatagttgtt aaaataacat aaatagccgt actcactcta tataaacaga
       61 aaagaaaggt taattattgg tgttggctat ataaagagta acaacagcaa tgtatatgaa
      121 taattaaagg ttatgcaacg ggcaaagatt aaatttccca gtttaatgac aggttgctgt
      181 acttgcgttt gttgctcttg ccggatgcgg cgtaaacgcc ttatccggcc tacagttcat
      241 tgcaaatcca ttcaatttca atgcacaatg taggcctgat aagcgtagcg catcaggcta
      301 atgtttaact tccctgttta atcagttact gctggcgtag ctggagatcc agcggcgttt
      361 tgctgggttc gccgccgatt tctcgcgcca gtttcggcac cagataaccc gacaccagtg
      421 tcagcaactc acgcataatc tgccgtgctt cgtcatcact caccataaaa tgcgccgcgc
      481 cctgtacttt atcgagcaca tgcaggtaat acggcattac gccggcatcg aacaacgcat
      541 tactcaggtt tgccagcgtt tgtgcgttat cgttcacacc acgtaacaga acgctctggt
      601 taagcagggt gacaccgaca cggcgcaatt tagccatcgc ctgacggaac gtttcatcta
      661 cctcattggc atgattgatg tgattcacca gcaagatttg cagcgtagaa cgggcaaagc
      721 gttcaaccag cgcctcggtg atacgtgccg ggatcacaat cggcagacgg ctgtgaatcc
      781 gcagacgttt gatatgcggg atggcttcca gttgtgtgag caaccagtcc agctcgtgat
      841 ctttcgccat cagcggatcg ccgccggaga aaatcatctc gtccagttcc ggatgcgcag
      901 caacatactc aagagcagtc tgccagttac gcttgttgcc ctgattttcg gcatagggga
      961 agtggcgacg gaagcaatag cggcaattta ccgcgcagcc gcctttgacc agcaaaagcg
     1021 cccggttgtg gtatttatgc aacaaaccag gcactacgct gtgctgttct tccagcgggt
     1081 cggtggagaa tccggacgcg acgacaaact catcttgcga ggtaagtacc tgacgcaaaa
     1141 gaggatcgtc cggattgcct ttctccatgc gatcgataaa tgagcggggc acacgcaggg
     1201 caaacagctt tttggcgctg cgtccggcca acagtttttc gtccgcgtct atattcaaaa
     1261 gacgcagaag ttcatcagga tcggtcacaa catcggcaag ttgcgttaac caatcttctc
     1321 tggatggggt atttagggtt acaatatgcg ccattttgtg gcttagctac caattaacaa
     1381 atttcagagg gccttatggc aacgtactat agcaacgatt ttcgtgctgg tcttaaaatc
     1441 atgttagacg gcgaacctta cgcggttgaa gcgagtgaat tcgtaaaacc gggtaaaggc
     1501 caggcatttg ctcgcgttaa actgcgtcgt ctgctgaccg gtactcgcgt agaaaaaacc
     1561 ttcaaatcta ctgattccgc tgaaggcgct gatgttgtcg atatgaacct gacttacctg
     1621 tacaacgacg gtgagttctg gcacttcatg aacaacgaaa ctttcgagca gctgtctgct
     1681 gatgcaaaag caattggcga caacgctaaa tggctgctgg atcaggcaga gtgtatcgta
     1741 actctgtgga atggtcagcc gatctccgtt actccgccga acttcgttga actggaaatc
     1801 gttgataccg atccgggcct gaaaggtgat accgcaggta ctggcggcaa accggctacc
     1861 ctgtctactg gcgctgtggt taaggttccg ctgttcgtac aaatcggcga agtcatcaaa
     1921 gtggataccc gctctggtga atacgtctct cgcgtgaagt aatgcggttg tggtgcggcc
     1981 tgcaggctgc accatcactt attcaggtca gagatgatga aacgccttat cgttcttgtt
     2041 ttgcttgcca gcacgctgct cacgggctgt aacaccgctc gcggtttcgg cgaagacatc
     2101 aaacatctcg gcaactccat ctctcgcgct gccagctaat ttttcttctc ttccgaaaaa
     2161 tcatcagatt tccgtcattt ttggcgatgt tgtctattat taatttgcta taggcaaaca
     2221 taaataacat tacctaaaag gaagacgtta tggtgaagaa gacaattgca gcgatctttt
     2281 ctgttctggt gctttcaaca gtattaactg cctgcaacac cacgcgtggc gttggtgaag
     2341 acatttctga tggcggtaac gcgatttctg gcgcagcaac gaaagcgcag caatagcaat
     2401 aagcaataac ggtacgacag gtatgtcgtg ccgtttgttt tttctgcgat agtcactaag
     2461 gtaatagttg aaattcccct gccacctggc aaaatatccg ttcaaccatc agctttgcag
     2521 gacgacctgc aaacgcctct tttcaccggg gacggcccca attctccgga gcctgatatg
     2581 tcctggatta tcttagttat tgctggtctg ctggaagtgg tatgggccgt tggcctgaaa
     2641 tatacccacg gctttagtcg tttgacgcca agtgttatta ccgtgacggc gatgattgtc
     2701 agtatggcgc tacttgcctg ggcgatgaaa tcgttaccag tagggacggc ttatgccgtg
     2761 tggacgggta ttggcgcagt cggcgcggcc atcaccggca ttgtgctgct cggtgagtcc
     2821 gctaacccga tgcgcctggc gagtctggcg ttaatcgtat tggggattat tggtctgaaa
     2881 ctcagcactc actaactacc gggctgctgt acccaaataa acttactgac atcaaaccct
     2941 tcccgggtcg cgactgccag catctcctgt ttcacttcgt cagaaatggt tggcgtgcgg
     3001 gagagtatcc acaggtagtc gcggtccggg ccgcaaacca gcgcatggcg gtattcccga
     3061 tcgagtgcaa taacgttata accgccatag aaaggaccaa agaatgacac tttcagcgca
     3121 gcgcgagttg gtgcgccggt aaagtacgct ttcccttcac tctgctgcca cattcctctg
     3181 tcagggttat agcctttatt aatgacattc aggccgccgt catcacgcag gctgtatgtt
     3241 gcggtgactt tttccagtcc acgttcaaaa cggtgatcaa aacgggcaat ctcataccag
     3301 gtaccgagat aacgtttggc gtcgaaatta ttcactactg ttacgccacg cggcggcgta
     3361 ggagaactgc aggcaacgac cagaaatgca gctgtcgctg cggcaacgag agggagcagg
     3421 cgcataaatg tttccttact ggtttttttc taagtgtaga tgacagcaag gaaaagcgca
     3481 gaaaaggtcc gaaaattcgg acccgatgaa attttactgt agagcgttga gaatctgcca
     3541 ggcggcggcg actctcgctg gattgggata gtttttgtta gccagcatca ctatacccag
     3601 ctctttttct ggaataaacg cgacatagct accaaatccg ccggttgccc ctgttttatg
     3661 tacccatgat gcgcgtactg caggagttgg gggcgtaatc gcttttacgg ggtgtgctgc
     3721 cagtgcaatt ttattgccac tgccgttaat gatgctgtca ggatttaccg gccagtccag
     3781 catttcccag cccaggccct gatacatatc gccggtttgc cagtagcgag attgtgccag
     3841 ttgtatccct tgctgaagtg ttttgtcgtt gatatcacgg ggattcatat tgcttcgtac
     3901 ccagcaggcc atatcttcaa tggtcgactt cacaccataa gtttcagcat ctaacgcccc
     3961 tggcgaaaca tgaactgcct tgccttcgcg atatccccag gcgtaattct tttcttctgc
     4021 gggcggtaca ttaatccacg tatggttgag tttgagtggc tggaagacac gtgtttgcat
     4081 cgcctgctca aaactcaaac cagacggctt cacagccagt gcgccgaaca aaccgatact
     4141 ggagttggca tacagacgtt gtgttcctgg cgcccatgca ggctgccagt tttgatagaa
     4201 gcgcagcaag tcgcttgagg atttcacctc atccggcacc tgcaatggca ggccgccagc
     4261 agtgtaggtt gcgagatgta atagtgtgat cccattccac tgtttagcgg taagttcagg
     4321 ccagtatttt gttgtgggat cgcttaactt gatttcccct cgagcaatag cgtcgccacc
     4381 aagcacgccc gtaaatgttt tgctgaccga acctaactca aacaacgttt gctgtgtgac
     4441 gggctgcttt ttggcgatgt ccgcatagcc ccaggtaaag taataaggtt taccctgata
     4501 aattaccgcc accgccatac cggggatctt ttgttgctct ataagcgggg taattgtgcg
     4561 atgcacaata tcgttgattt gttgaggggc agcaaatgtg gagcaagagg cggtaattaa
     4621 taaggcgcag agcgtcgttt tgaacataag gtctggtttc catacaaaac ggcccgccat
     4681 aggcgggccg gatttacatt ggcgatacgt tagattgtaa cgataccaat cagcgtgaca
     4741 actgtcagga tagcagccag accgtagaaa acccatttgc ccgcaggcac gtgaattttc
     4801 agatcgtgca tcgcgtggtg catacggtgt aaaccacacc acagcggcag aacgatcatc
     4861 aggaacagga atacgcgacc aatgaagctc tgcgcgaacg ccagaacgcg ctcgtagctc
     4921 agcgcatcac ccggaaacag ccccagtggc agcagaatac ccaccagcag gatcatcacc
     4981 ggcgcaataa ttgcgctcca cataccaccg gccccgaaga ggccccagaa taccggttcg
     5041 tcagaacgct ttggatttgg attaatcatc tcaggctcct taccagtaca gggcaacaaa
     5101 caggattacg atggtggcaa ccacagttac cgcccagaga cttttgataa ttggctctgg
     5161 tcccattttt tcgtctttta caatgatatt ggccgctttc ggtgccagtt caaaccaggt
     5221 tttggtgtgc agcagagctg ccgccagagt gatcaggtta atgatcacga taaccgggtt
     5281 ttgtaaaaag tcgacgaatc ccgcccaggc ttccgggcca tttttcaggg caaacagccc
     5341 gaaaatcagt tcaatgctga accacacagc cggaaccgcc gtgccttcgc gcagcatgta
     5401 aaagcgataa aacggcaatt ttttccacca ggtggacgtc attggccgta catacggttt
     5461 acgtttagtc gtcatgttgc actccttagc gtggtttcag ggtcgcgata agaaagtctt
     5521 tcgaactttc tactttgccc tgctgaatgg ccgcagccgg atcgacgtgt ttcgggcaga
     5581 cttcggagca atagcccacg aaagtacagc tccatacgcc gttctggctg ttcaactgcg
     5641 ccatacgctc cttcttaccg tggtcgcggc tatcttcgtt ataacgatgc gccagcgtaa
     5701 tggcagccgg accgatgaac tctgggttca ggccaaactg cgggcacgcg gcgtagcaca
     5761 gaccacagtt gatgcaaccg gagaactggt gatactttgc catctgcgcc ggggtctgga
     5821 tgttagtacc ctgatccgcg gtgcgggagt tgccgatgat gtacggtttg atcgcttcca
     5881 gactttcgat gaagtgggtc atatcgacca ccagatcgcg ttcaatcggg aagttagcta
     5941 acgcttcaac cttcataccg tcggtgtaat cacgcaggaa ggttttacat gccagttttg
     6001 gcacgttgtt aaccatcatg ccgcaggaac cacaaatcgc catacggcag gaccagcggt
     6061 aactcaggtc cggtgccagg ttgtctttga tgtagcccag cgcatccagt aatgaggtag
     6121 ttgcgtcata aggcacttca tagaatgcgc tatgcggtgc ggtatcgact tccgggttat
     6181 agcgcaccac ctcaattttc aggtttttca tctcagccat tcgccttctc cttcttattg
     6241 gctgcttccg ccttatcggc tgcatccgct tcgccaccgt aaacgcgttt agctggcggc
     6301 agcgtagtaa tcttcacgtc gctgtactcc aggcgagtcg tgccatcagc atcgcggaag
     6361 gcgagggtgt gtttgaggaa gttgacgtcg tcacgctcgg tgcaaccttc gtccagacgc
     6421 tggtgtgcgc cgcgggactc tttacgtgcc attgcggagt gcgccataca ttcagcaacg
     6481 ttcagaccgt ggcccagttc aatggtgtag agcaggtcgg tgttgaacac gctggaggtg
     6541 tcggtgatgc gcacgcgctt gaagcgttcc tgcagctctg ccagcttgtc gatggttttc
     6601 tgcatcagtt ccggcgtacg gtagataccg caaccttctt ccattgccag gcccatttcg
     6661 tcgcggatct tcgcccagtt ttcgccgcca tcctggttaa ccagatcttt cagacgttgt
     6721 tcaacgccag ctgcctgcgc ttcaattgcc gcttcgttgc cattaccggc agttgctgca
     6781 cgctctgtcg cttgttcacc ggccagacgg ccgaagacca ccagttccgc cagggagtta
     6841 gaacccagac ggtttgcacc gtgcagacca acagaggaac attcacccac ggcgaacaga
     6901 cctttaatgc gggtttcaca gttctgatcg gtttcgatac cgcccatggt gtagtgtgcg
     6961 gtcggacgta ccggaatcgg ttctttaacc ggatcgacgc caacgtacgc tttcgccagt
     7021 tcgcagatga acggcagacg ttcatgcagt tttttctcgc cgaggtgacg caggtcgaga
     7081 taaaccacat cgccacgcgg cgtggagatg gtgttgcctt tacgccattc gtgccagaag
     7141 gcctgagaaa ctttgtcgcg tggacccagt tccatatatt tgtttttcgg ctcgcccagc
     7201 ggagtttccg ggcccatgcc gtaatcttgc agataacggt agccattttt gttgaccaga
     7261 ataccgcctt caccgcggca gccttcggtc atcaggatac cggaacctgg cagaccggtt
     7321 gggtgatact gaacgaattc catgtcacgc agcggaacgc cgtggcttag cgccataccc
     7381 ataccgtcac cggtaacgat gccgccgttg gtgttgtaac gataaacgcg acccgcaccg
     7441 ccagtagcca taacgaccgc gttagcacgg atctgcacca gcgtgccttc catcatgttc
     7501 attgctacca cgccgcgaac atgaccatca tcaaccagaa tatccagcac gaaatgttcg
     7561 tcaaaacgct ggatctgcgg gaattgcaga gaggtctgga acagcgtgtg cagcatatgg
     7621 aagccggtct tatcggcggc gaaccaggtg cgctcgattt tcatgccgcc gaagcgacgt
     7681 acgttgacgc taccatccgg gcgacggctc catgggcacc cccacagttc cagttgggtc
     7741 atttcggttg ggcagtggtg gacgaaataa tccacgacat cctgctcaca caaccagtcg
     7801 ccacccgcta ctgtatcgtg aaagtgatat tcgaagctgt catgatcctg cgcgacagcg
     7861 gcggagcccc cttctgcagc aacggtatgg ctacgcatcg ggtatacttt tgagattagt
     7921 gcgatttttg catttggatt tgcctgcgcg gcagcaattg cagcacgtaa tcccgcgcca
     7981 ccggcgccta caatggcaag atcggcttga aaggtttgca cgacattcct ccagattgtt
     8041 tttatcccac agccccgtac ttcagggtaa gtgcctgaaa gttacggtct gcgaacgcta
     8101 ttccactgct cctttatagg tacaacagta tagtctgatg gataagtctg aaatttgacg
     8161 agatcgattt ttttagtgcg caagagggct aaattatcac tgaagatgat taatttaatt
     8221 actaaaccat cagatcgtgc tttttttagt cactgcccac cgctgtttga ttcctgcgta
     8281 aaatttgtct cgccgtcgcg ttgcgagtag acttcgtgcc cttgtcaaaa actggagatt
     8341 taactatgag cgaaacggca tcctggcagc cgagcgcatc cattcctaac ttattaaaac
     8401 gcgcggcgat tatggcggag atccgtcgtt tctttgccga tcgtggagtg ctggaggtgg
     8461 agacgccttg tatgagccag gcgacggtaa ccgatattca tttggtcccg tttgagacac
     8521 gtttcgttgg ccccgggcat tcgcagggga tgaatctctg gttaatgacc agcccggaat
     8581 accatatgaa acgcctgctg gttgccggtt gtgggccggt attccagctg tgccgcagct
     8641 tccgtaatga agagatgggg cgttatcaca accctgagtt cactatgctg gagtggtatc
     8701 gaccgcacta tgatatgtac cggttgatga acgaggtgga cgatctctta caacaggtgc
     8761 tggactgccc ggcagcagaa agcctttctt atcaacaagc tttcttgcgt tatctggaaa
     8821 ttgacccgct ctctgccgac aaaacgcaac tgcgggaagt cgcagcgaaa ctggatttga
     8881 gcaatgttgc tgataccgaa gaagaccgcg acacgctgct acaattgctg tttacctttg
     8941 gcgtagagcc aaatattggc aaagaaaaac cgacctttgt gtaccacttt ccagccagcc
     9001 aggcatcact ggcgcaaatc agtaccgaag atcatcgggt cgctgaacgc tttgaggttt
     9061 attataaagg tattgagctg gcgaatggtt tccatgaatt gacggatgcc cgtgagcagc
     9121 aacaacgctt tgaacaagat aaccgtaagc gcgcggcgcg cggtttgccg cagcacccca
     9181 ttgaccagaa tctgattgaa gccttgaaag tcggtatgcc tgactgttcc ggcgtggcat
     9241 taggtgttga tcgtctggtg atgttggcgc tgggcgcgga gacactggct gaagtcatcg
     9301 cctttagcgt tgaccgggca taattctgaa aaattactga attaacagcg aagaatggcg
     9361 tgataagcgc cattctgtag catatgtttt catttttccg ttaaaggcca caatttaaga
     9421 ctggatattt gatatcatcc aggtatcaat tctgtattgg tttttacgct gcctgctcaa
     9481 tgttgcgcag tgcagttcag tgggcacgtg ttatacacgc gctgaaatga aggatggttt
     9541 catgcctcac acgataaaaa agatgagtct gataggactc atattgatga tctttacttc
     9601 cgtatttgga tttgccaata gcccatcggc ttattactta atgggttata gtgcgattcc
     9661 cttttatata ttttctgcat tgttattctt tattccattc gccttaatga tggctgaaat
     9721 gggagctgct tatcgcaaag aagaaggcgg tatctattcc tggatgaata atagtgtcgg
     9781 accacgtttt gccttcattg gtacgtttat gtggttttcc tcttatatca tctggatggt
     9841 gagtacctcc gcgaaagttt gggtaccgtt ctcaacattc ctctatggta gcgacatgac
     9901 ccagcactgg cgtattgctg gactggagcc tacgcaggtg gttggtctgc tggcagtggc
     9961 atggatgatt ctggtcaccg tcgttgcttc aaaggggatt aataaaattg cccgcattac
    10021 tgcggtgggc ggtattgcag taatgtgtct gaatttagta ttgctgttag taagcattac
    10081 tattttgtta ttaaatggtg ggcatttcgc gcaggatatt aatttccttg catcaccgaa
    10141 cccaggttat cagtccggtc tggcaatgct atcgtttgtg gtatttgcta tttttgccta
    10201 tggcggaatt gaagcggttg gtggtctggt cgataaaacg gaaaatccag aaaagaactt
    10261 tgccaaaggt attgtttttg ccgctattgt tatttcaatc ggttattcgc tggcaatatt
    10321 tttatggggc gtcagcacaa actggcagca ggtattaagt aatggttccg ttaaccttgg
    10381 caatattacc tatgtgctga tgaagagcct cggggtgacg ctgggtaacg cactgcattt
    10441 gtcacctgaa gcgtcattgt cgctgggcgt atggtttgcg cgtattaccg gactttcgat
    10501 gttcctcgct tataccggtg cgttctttac gctttgctat tcaccgctga aagccatcat
    10561 ccaggggacg ccgaaagcgt tgtggccgga accgatgacg cgcctgaatg cgatggggat
    10621 gccgtctatc gccatgtgga tgcagtgcgg gttggttact gtcttcatcc tgctggtttc
    10681 gtttggtggc ggtaccgcat cggcgttctt taacaagctg acgctgatgg cgaacgtgtc
    10741 tatgacgctt ccttacctgt tcctcgcgct ggctttccca ttttttaaag cacgtcagga
    10801 tctcgacaga ccatttgtga ttttcaaaac gcatatgtcg gcaatgattg caacagtggt
    10861 tgtcgtactg gtggtgacat ttgcgaacgt cttcaccatc attcaacctg tggttgaagc
    10921 cggagactgg gacagcacat tgtggatgat tggcggccct gtcttcttct cgctgttagc
    10981 gatggcgatt taccagaact attgcagcag agtggcaaaa aatccgcagt gggcggtgga
    11041 ataacccgat gccctatcgt ctacctgacg atagggcgta aagacaggaa atgttatgga
    11101 tgacaccagc cgggacccgg caataaccga agatgaaatc agggcgctgc aattttctgc
    11161 cggggatgta gcggaaatag agcagacgat tctctctttt gtcgatgcct gccacacccg
    11221 taaagtggcg atggtggtgg gtagcaccat taacaccctc aaagatcgcg atggtaaacg
    11281 gtggggaaac ctgccggata tttattgcgc ttatcttatc cgatgtctgg tttttcgcgg
    11341 ggaattagtg ggatacggcg atttgtttcg tatgcgctat agcgaaatta agcgaccggt
    11401 taccttatga gtgcgcgcat gtttgtctta tgctgcatct ggtttattgt ggcgttcctc
    11461 tggataacca tcacttccgc gctggacaaa gagtggatga ttgatggacg cgggattaac
    11521 aacgtctgcg atgtgttgat gtatctcgaa gaagacgata cccgtgatgt tggtgtgata
    11581 atgacgttac cgctcttttt cccttttctc tggttcgctt tgtggcgaaa aaaacgcggc
    11641 tggtttatgt acgccaccgc gctggctatt ttcggctact ggctgtggca gttttttctg
    11701 cgctatcagt tttgtttgtg agccggattg gttcatccgg cacacaaact tacaaacttc
    11761 ccgcctgacg acctttgccc gcagacgtca gcgttctccc cgtttgttta ccgttgaggc
    11821 tttccagacg catctggaag ggcgggaatg gcatatcgat accgtgggcg tggaagcctg
    11881 ccagaatcag ctggtggatc tcatggcgta gcggcatacg gtgacccatc tcagcggcgt
    11941 aaatacgcag ctcgaaaatc tgaatcccct gttgcagatc caccaggaag acttccggtg
    12001 ccgggttgtc gatcaccagc gagcagcgac gcgctgcggt gagcaggatt tccgtcactt
    12061 cttcgctatt ggcatcggca ggggccggta tcgtcaacac cacgcgcgtg accgagtcag
    12121 agagcgacca gttgataaac tgctcggtaa taaacgcctt gttcggcacg attatctctt
    12181 tacggtccca gtcgctgatg gtggtggcgc gggtgttaat tttcgtcacg ctaccggtaa
    12241 gatcgcggat cgttaccgta tcgccaatgc gaatcggttt ttcgaacagg ataatcaggc
    12301 cagagataaa gttggcgaaa atctcctgca aaccaaaacc gagaccaaca ccgagcgcgg
    12361 caaccagcca ctgcaatttc gaccactcaa taccaatcat cgagaagccg accagcccgc
    12421 caatcagcat cagcagatat ttggtgatgg tggtgatggc gtagcccgta cccggcgtta
    12481 aatccaggtg ctgcaaaatc gccagttcca gcagcgcggg caggttgcgc accagctgcg
    12541 tggtgatgat aaacaccaga atggcaatca gcaccgcacc gagggtaatt ggctccagac
    12601 tttctacgcc ctgtaccgtg gaggtgacat cccacagcga aatattttcg aggaagccga
    12661 aagcggaatg gatttctgac cacagcacaa tgacagaaag cagggcgatc aacatcaaaa
    12721 ttgagcgcac cagccgcaag gattgcgcac tgatggcatc gagatcgact tcgctttcat
    12781 cgacttcaat tgccccttcc gggctactgt gatgatgcgc ttcctcttcg ccacgcgcgc
    12841 gttgcgctaa catctctgcc cggcgatgct tcgcccggtc aaacgccagc ctgcgacgct
    12901 ggatcagcat ccagcggcgg ataacgtgat aaaccaccag tagcaggaac cagatggcaa
    12961 ccgaggtttc aagcctcgct aacagtgcct gcgccgttgc cagataaccc accgctgacg
    13021 ccagaatggc aaccagtggt gcgccaatca tcatgttcca cagcatatgg ttggtaatgt
    13081 tgtcgccgct gccctctttg ttgaggtaca gcgggatccc ggcctttttc aggctgaggg
    13141 tgaccaccgc cagcgcaccg caaatgagga taaagcaaag ccgtcccagc gaaccggaga
    13201 attcacggtc gtcgaggtta tcgaacatca tcagcgccat aatcagcggc acaataagcc
    13261 cgatgctcat caggtagtag cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat
    13321 gagcgataaa caagccgttc gggcgggcaa aggtggcgca aatcatcact acccacagca
    13381 gcggcacggt ggccgttacg ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct
    13441 cgcgcaagcc gtaacccagc gtcatccaca gcaccggcag cggtgacgcg acgagaatcg
    13501 accagaaaag agtgcgcaac gtcagccaga agtgatcctg agtcactttg ccgactttcg
    13561 ccgccgaacg ttcaagaaaa cgggtgaaat agcggcgcga gtaaatactg caaccgacca
    13621 gaatcaacgc gccaaacagc ggcaaaatcg tctctttgct ggtcagcatc atcacactgg
    13681 ctttgcccaa ctgactgaag gtgtccagcg aaatgagacg acgcagatcc tgggcgattt
    13741 ccagtggcca ggcgatggtc atcgggcgca cgtcagaggt ccagaacaga tagcgatgcg
    13801 tcgcttcatt cacctctttc agcgcatcct caagttgccc gttggagact ttcagcttgg
    13861 tcagttccag cagtagcgtg tcgccaccct gcaacaatga gttcagcaac tcacgctgag
    13921 tgcgtagctg tgcttccaga atacggtttt gctcggcggt cagcggctga ccgtcggcct
    13981 gatgaatttg ccgtagcagc ggctgtttat taagcagatc ctcataacgt aaccgttgca
    14041 cacgcaactg cgccatttcg gtatcaagct gttgtggttt cggcatttcc ggcagccgtg
    14101 ccacctgcgc ccgcaacgct tcgccgagca ggttggacga tcccagccat tgcgactgtt
    14161 cacgcagcgt attcagcgcc tgccgaacct gtaacgtctg gctggtggcc tggcgctgtt
    14221 gcgaggcaac gagatccatc cgctgcgcct gttgattcag tgccgccgat agttcgcggt
    14281 taattttgaa ttgcgcgacg atgtctttcg gcaaatcggc gctgttttct gccagcaatt
    14341 cggtactttc cagcgcccgc tccgcctcaa gctgacgttg gctgttcaac tgattacgta
    14401 aggcctgcaa atacgcatcc agttgctggc tctctttttc cgccagctct gagcgtaagc
    14461 gcgctaattc ctgacggtta ttggcagaca gctgcgccag ttccagttca tcaacgagcg
    14521 ctttcaggcg tgcggagtca gactgcaacg cgaaattttg tgcctgattg agcggggtat
    14581 tgccagtaag cgttcccagg cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt
    14641 gctgttgcgg cagttgattc agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct
    14701 gggcctgacg gcttttatcc agcaactggc tgctgacctg gagaatttcc tgattcagcg
    14761 cgtcggtaga cattcccggc gacacgctgc gcggctcgtc acgcatgttg tttaattgtg
    14821 cgcgcagagt agcggagagt ttcggataat tatcgataac ttgctgatat tgtttgatgc
    14881 gctcaaggga accttttcgt tcctcaagcg catttaaggc agactggagc gcctctacga
    14941 cttccggctg tgcgggtttc gccgcttttg cctgctccag ttcctgagtg atttgtttgc
    15001 tatcgggggc cgtcgcggcg tacgcccccc aactgaggca ccaggccatc agaaaagtga
    15061 taatcaggcg cacgtcagcg tttcctttga tggattagac ctggtctttt ttatcgtcaa
    15121 ccaatgggct ggcgtcgtgt tctgcttcga tctcttcagc aggaagtggg gcgggttcag
    15181 cgtctggcgt aacaaaggtt tcggtagata ctgccagcgg ctggccaatt ttcgtgacag
    15241 acaggctttc cagttgctca accagattca ctttacccgg tgcaaacagg ttgataacgg
    15301 tagagccgag tttaaagcgg cccatttcct ggcctttcag cagtgccaca gaaccgtcgt
    15361 tttccccggc aggccaggtc cagcgcttga tgataccttc gcgcggcggc gtaatggtgc
    15421 ccgcccagac cgtctcaatg ctgcctacaa tcgtcgcccc gaccagaatc tgcgccatcg
    15481 ggccaaattc ggtatcgaac aggcaaatca cgcgttcgtt acgggcaaac agattcggca
    15541 cgttctgagc ggtgagatgg ttaacggaga agagatcgcc cggcacgtag atcatctcac
    15601 gcagaatacc gttgcacggc atgtgtacgc gatggtagtc acgcggggag aggtaagtgg
    15661 tcacaaacgt accgttgcgg aacaggtccg ccatcagata gttgcctgcc agcagggctt
    15721 cgaggctgta gttgtggcct ttggcttgca ggattttatc ttcttcgatt ttacccaact
    15781 ggctgataac gccatcggca ggcatgacca ggacattcgg atcggtatcg attgggcgta
    15841 cttcgtcacg cagcggacgg acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg
    15901 gcttttgcgc ctctttcatg tcgaccttgt agtatttaac gaacagatcg ataaccagtt
    15961 ttgtcagcca tcctgcccgc ttgcttgcgc cccaacccgc caggcgagta agccatagtt
    16021 tcggcagaat gtactgtagc gaaagtttaa atgaatttaa caaggtagcc tccaggccat
    16081 tgttttgtcg ttcctgatcc ggccaacatg ccggatcctg aaaaaaaggg gacgattcta
    16141 acgacggtta gcttaattgt cagtcatccg tatcagaaaa gtttttacgc gtttttacct
    16201 gcgccatgct ttccagaata cggtgatagt tttcgaaacg ggtttccgcg attttccctt
    16261 cctcaaccgc ttcccggata gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt
    16321 tacacagacc taaatagtca tggaattcga caaagccctg agtgatttgt tccggctcca
    16381 ggtgccagag gccgaactca cgcacaccag gggaatcaat cacatcaccg ccgtgcggga
    16441 agtgatacag ccgagcggcg gtcgtggtgt gctgaccgag ccccgagttg tcagagacat
    16501 cgttggtcag gatctctttt tgcagcccca gcaacgcatt cagcaggctg gatttgccga
    16561 cgccagactg cccggcaaaa atgctgatgc gcccggtcaa cgcctcttcc agcggtttta
    16621 gcccatcctg agtatggctg gaaaccatca atacgcgata accgatattg cggtagatat
    16681 ccatctgctc gttgacgaac gccatgcctt cgtcgtccag caggtctatc ttgttgagca
    16741 caataatcgg ctcaatctgc aaggtttcgc aggccaccag gtaacggtcg ataatattga
    16801 gcgacagctc cggcaaaatg gcggagacaa tgacaatctg gtcgatgttg gcggcaatag
    16861 gttttacgcc gtcgtagaaa tccggacgcg tcaacaccga ggtacgctca tgcaccgctt
    16921 ccacgatccc tttgacattt acgccttccg ccgccggttt acccggacgc cagactacgc
    16981 ggtcgccggt taccagcgaa cggatggtac ggcgaatatt gcagcggtga acgtcgccat
    17041 cggcggattc cacatcagcg tgcataccaa agcggctgat gacgatacct tcatcaggct
    17101 cgccaaacag attgtcgtcg tagtcgggct tctccttaga cgttttaaga cgacgctggt
    17161 gattggcgtt cacgcggcgc tgctggcctt tggagagttt atttttactc aatcgtacag
    17221 actcctggtc gcccctgatg ggcaaaacat ctatgataca cgcaattgtg gatcaatata
    17281 gtcactgtga atgggtggaa aatagcatga gtgccaatga aaacaacctg atttggatcg
    17341 atcttgagat gaccggtctg gatcccgagc gcgatcgcat tattgagatt gccacgctgg
    17401 tgaccgatgc caacctgaat attctggcag aagggccgac cattgcagta caccagtctg
    17461 atgaacagct ggcgctgatg gatgactgga acgtgcgcac ccataccgcc agcgggctgg
    17521 tagagcgcgt gaaagcgagc acgatgggcg atcgggaagc tgaactggca acgctcgaat
    17581 ttttaaaaca gtgggtgcct gcgggaaaat cgccgatttg cggtaacagc atcggtcagg
    17641 accgtcgttt cctgtttaaa tacatgccgg agctggaagc ctacttccac taccgttatc
    17701 tcgatgtcag caccctgaaa gagctggcgc gccgctggaa gccggaaatt ctggatggtt
    17761 ttaccaagca ggggacgcat caggcgatgg atgatatccg tgaatcggtg gcggagctgg
    17821 cttactaccg cgagcatttt atcaagctgt aaaatttttc ctggtcacgt aagcgccgga
    17881 atccacttgc ggcggcgtga ttttgacgct aaattgaacg tcttgctgat aatttcagca
    17941 cttgagataa aaacgcaaaa aaaacttttt tggggggttg cagagggaaa gatttctcgt
    18001 ataatgcgcc tcccgtaacg acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt
    18061 acgcgggaat agctcagttg gtagagcacg accttgccaa ggtcggggtc gcgagttcga
    18121 gtctcgtttc ccgctccaaa atttgaaaag tgctgcaaag cacagaccac ccaatcgcag
    18181 tatttcgaat tgcgacaagg cggcaactga acgaatcctt gggaacttac aaaagtatgt
    18241 gactaaggtg agtgaaggca gccaaggcag tagcggttcg aaagacgaag attaagcggg
    18301 aatagctcag ttggtagagc acgaccttgc caaggtcggg
//
LOCUS       DAZGOB010000092        18340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGOB010000092 DAZGOB010000000
VERSION     DAZGOB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.092"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..164
                     /gene="xisR"
                     /locus_tag="ACHLIF_003416"
     CDS             <1..164
                     /gene="xisR"
                     /locus_tag="ACHLIF_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO9607375.1"
                     /translation="KKAREKSFMEGREYKHVAHDGMPWDNSPCFYNLEEIDRWIERQA
                     SARPRRHLT"
     gene            199..1479
                     /locus_tag="ACHLIF_003417"
     CDS             199..1479
                     /locus_tag="ACHLIF_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9607376.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTSKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSKFAPTMPHNEAMKN"
     gene            complement(1667..2686)
                     /gene="rspB"
                     /locus_tag="ACHLIF_003418"
     CDS             complement(1667..2686)
                     /gene="rspB"
                     /locus_tag="ACHLIF_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO9607377.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(2698..3912)
                     /gene="rspA"
                     /locus_tag="ACHLIF_003419"
     CDS             complement(2698..3912)
                     /gene="rspA"
                     /locus_tag="ACHLIF_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO9607378.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4118..4444)
                     /gene="ynfA"
                     /locus_tag="ACHLIF_003420"
     CDS             complement(4118..4444)
                     /gene="ynfA"
                     /locus_tag="ACHLIF_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO9607379.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4579..4920
                     /gene="ynfB"
                     /locus_tag="ACHLIF_003421"
     CDS             4579..4920
                     /gene="ynfB"
                     /locus_tag="ACHLIF_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO9607380.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            4955..5515
                     /gene="speG"
                     /locus_tag="ACHLIF_003422"
     CDS             4955..5515
                     /gene="speG"
                     /locus_tag="ACHLIF_003422"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO9607381.1"
                     /translation="MPSAHSVKLRPLEREDLRYEHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5518..6228)
                     /gene="ynfC"
                     /locus_tag="ACHLIF_003423"
     CDS             complement(5518..6228)
                     /gene="ynfC"
                     /locus_tag="ACHLIF_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO9607382.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6336..6641
                     /gene="ynfD"
                     /locus_tag="ACHLIF_003424"
     CDS             6336..6641
                     /gene="ynfD"
                     /locus_tag="ACHLIF_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO9607383.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            6840..9265
                     /gene="ynfE"
                     /locus_tag="ACHLIF_003425"
                     /pseudo
     CDS             6840..9265
                     /gene="ynfE"
                     /locus_tag="ACHLIF_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            9326..11749
                     /gene="ynfF"
                     /locus_tag="ACHLIF_003426"
                     /pseudo
     CDS             9326..11749
                     /gene="ynfF"
                     /locus_tag="ACHLIF_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
     gene            11760..12377
                     /gene="ynfG"
                     /locus_tag="ACHLIF_003427"
     CDS             11760..12377
                     /gene="ynfG"
                     /locus_tag="ACHLIF_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO9607384.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            12379..13233
                     /gene="ynfH"
                     /locus_tag="ACHLIF_003428"
     CDS             12379..13233
                     /gene="ynfH"
                     /locus_tag="ACHLIF_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO9607385.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            13276..13890
                     /gene="dmsD"
                     /locus_tag="ACHLIF_003429"
     CDS             13276..13890
                     /gene="dmsD"
                     /locus_tag="ACHLIF_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO9607386.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            14049..15341
                     /gene="clcB"
                     /locus_tag="ACHLIF_003430"
     CDS             14049..15341
                     /gene="clcB"
                     /locus_tag="ACHLIF_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO9607387.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(15294..15989)
                     /gene="bioD"
                     /locus_tag="ACHLIF_003431"
     CDS             complement(15294..15989)
                     /gene="bioD"
                     /locus_tag="ACHLIF_003431"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO9607388.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(16114..17334)
                     /gene="mlc"
                     /locus_tag="ACHLIF_003432"
                     /gene_synonym="dgsA"
     CDS             complement(16114..17334)
                     /gene="mlc"
                     /locus_tag="ACHLIF_003432"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO9607389.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(17469..>18340)
                     /gene="ynfL"
                     /locus_tag="ACHLIF_003433"
     CDS             complement(17469..>18340)
                     /gene="ynfL"
                     /locus_tag="ACHLIF_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9607390.1"
                     /translation="RYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLAR
                     TNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVS
                     DTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRYTALPETLEHAVIVHEPL
                     MAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEV
                     GEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPA
                     ARNFRIHLLNALR"
BASE COUNT         4759 a         4635 c         4432 g         4514 t
ORIGIN      
        1 taaaaaaggc ccgggaaaaa tcgtttatgg aaggaaggga atataagcat gtcgctcatg
       61 acggtatgcc atgggataac agtccatgct tttataacct ggaagaaatt gatcgctgga
      121 ttgagcgcca ggcatcagcg agaccaagac gtcatcttac ttgactaaaa gccacactaa
      181 ctaatgagag aagttgaaat gaaatatccg acaggcgtgg aaaaccatgg agggaaatta
      241 cgtatctggt ttgtttataa aggcgtaaga gtcagggaaa atctgggggt tcctgacaca
      301 tcaaaaaaca ggcgcgttgc aggtgaacta cgctcctctg tttgttacgc aataaaaact
      361 ggtgttttcg actatgcaaa acagtttccc tcctcacgca atctggaaaa atttggtgag
      421 gcccgacaag atttaaccat aaaagaactg gctgaaaaat ttttggcact gaaagaaact
      481 gaagtcgcca aaacatcact caacacatac cgtgccgtca tcaaaaatat cctgagcata
      541 atcggtgaaa aaaatcttgc ctcatcgatt aataaagaaa aattactgga ggttcgtaaa
      601 gagctactga ctggatacca gatccccaaa agtaactata ttgttacaca accagggaga
      661 tcggctgtaa ctgtaaataa ttacatgaca aatcttaacg ccgtgttcca gtttggtgtt
      721 gataacggtt acctggcaga taatccgttt aaggggatct cgccattaaa ggaatcaaga
      781 accattccgg atcctctttc gcgggaggaa tttatccgtc ttatcgatgc gtgcagaaat
      841 cagcaagcaa aaaatttatg gtgtgtttct gtttatactg gcgttcgccc tggtgagctg
      901 tgtgcacttg gatgggagga catagatctg aaaaatggaa caatgatgat caggagaaat
      961 ttagcaaaag accgtttcac ggtaccaaaa acacaggcgg gaaccaatcg ggtcattcat
     1021 cttattaagc cagcaatcga cgctctccgg agtcagatga cattaacgag actgagcaaa
     1081 gagcatatca ttgatgttca cctcagagag tatggcagaa cagaaaaaca aaaatgcacc
     1141 tttgtttttc aacctgaagt gtcagcgaga gtaaaaaatt atggtgacca ttttaccgtt
     1201 gactcaataa ggcagatgtg ggacgcagcg ataaaacgtg ccggactccg ccatcgaaaa
     1261 tcatatcagt cgagacatac ttatgcctgc tggtcgctga cagctggtgc taacccggca
     1321 tttatagcaa accagatggg ccatgcagat gcgcaaatgg tatttcaggt atacggaaaa
     1381 tggatgtctg aaaacaataa tgcacaggta gctttgttaa atacacagtt aagcaagttt
     1441 gccccaacca tgccccataa cgaagcaatg aaaaattaat ttaatattta tcaaatagtt
     1501 aacacgcatg actcttgaaa tccataaatt caagcgcagt gcccagccat cccgatactg
     1561 ctgctttcac caaatcctta gtgcttcttt catgtttttc tattgtcata atggttatct
     1621 ctaaaaaaga ggtaagatgc gtactactta ctcgccgtta ttggtattat tcagaaaaag
     1681 tgagcaagac tttgcagcaa tgtttttgat cctgttcaaa taaactaatg gcatcagcaa
     1741 catgctggaa atcaaacgta tgggtaatta atttttctgg tttaattaac cctttactta
     1801 accagtcgat aacaaccgga aatttatttg catttaagcg tgaagagaaa atagagagtt
     1861 cttttccggt aattccttgc tgaatcactt cagacggttc actggagaag cccatcaata
     1921 caatacgtgc cgctggagaa gccagcgtta cggcctcttt caggatagaa ggatgacaag
     1981 ccgcatcgat aattaatgtc ggcttgatgc ctttttcagc gaaaatctcg ccaagcggtg
     2041 tctggctgtt attaatcgcc cagtctgccc cgctctcttt cgctttttcc agtcgttcat
     2101 caatgcgatc ggcaacaatc acatttttaa cgttatagac gccttttaat acctgaacga
     2161 tcgtcaggcc gattggaccg gcaccataaa ccagaacggt atcattttca gtcggttgac
     2221 catgaccggt aacgttagcc gcaatggtaa aaggttcgat catcaccgca tattgatcgg
     2281 ccactgcttc aggaattttc cacgcatttt ttgccggaac cacggcatat tcactgaaac
     2341 caccgtcagc gtgcacacct aatacagcaa gtgtcgtaca aacgttcggc ttacctatag
     2401 agcacggata gcaatgccca cagctgacca ccggatcgac agcaacgcgc tcaccgactc
     2461 tggcgctttc cacaccttca cccaccgcat caatgacgcc aaagaattca tgaccaatga
     2521 cgcgcggata tttcgcaaaa ggattatgcc cacggtaaat atggctatct gaaccacaaa
     2581 ttccggcaag tttcactttt actcgtactt cacccgctga cggggtgggt atttcacgtt
     2641 cgataatcga cagttgattc ggtttttcaa ttaaaatgct tttcattacc ttactcctta
     2701 ccagttccac agcgtgccat cttccagacg tgcgactggc agataagcag gttcataggg
     2761 atatttcgcc gccagctttt catcgaattc gatgccaaga cccggtttgt ctcccggatg
     2821 catatagccg ttatcgaaag tccagttgtg cgggaagact tcgagcattt gttcggaata
     2881 acccatgtac tcctggacac cgaaattggg gacccacaga tcaaagtgca gcgccgcagc
     2941 catgcagact ggtgacaaat cggaaggacc gtgtgagcca gtacgtacct gatacagcga
     3001 agcaaaatcg gcaatccggc gcataccggt aattccgcct gcatgggtca gcgtggtgcg
     3061 gatataatcg atgagttgct cttcaatcag ttgtttgcag tcccagatgc tgttgaagac
     3121 ttcacccact gcgatgggtg tgacggtatg ttggcgaatg agacggaagc attcctggtt
     3181 ttccgcaggc gtcgggtctt ccatccagaa catgcgataa tcttcaatgc ttttaccaaa
     3241 gcgcgccgct tcaataggcg ttaagcgatg gtgcatgtca tgcagcaaat gttcattaaa
     3301 accaaacttg ttacgtaccg cgtcaaacaa tttcggcatg aaatcgaggt atttctccgt
     3361 cgaccacagc tgctcttccg gccactgtcc tttggttgcg ggttcataag ccagaccttt
     3421 acctttcgac atgccgtagg tggttttcat accagggatt ccgcactgca cgcggatggc
     3481 tttgaatccc agctcctgat gacgggcata atcatccaga gcttcatcaa tactgtgacc
     3541 ggtggtatgg caataaacca tcaccccttc acgagacgcg ccgccgagta actggtaaag
     3601 cggcatgttg gcagctttgg ctttaatatc ccacagcgcc atatcaaccg ctgaaatggc
     3661 cgacatcgta accggaccgc gacgccagta agcaccttta tagaaaaact gccagatatc
     3721 ttcgatacgg tgcgcatcgc gaccaataag ctgcggacaa aggtgatcct gcaaataaga
     3781 ggccacggaa agctcacgtc cattgagggt ggcatcccca aggcccgtaa taccgtcctc
     3841 agtggtgatt tttaatgtga cgaaattacg ccccggacag gtaacaaaaa cttcagcctt
     3901 tacgatcttc atgttcgatt ccttgcatcg cttgtcgtga tgcatgaaat ctacgcaact
     3961 gagctactac catacaagca taaagatcga aaaaagccgg agtgatcaca aaaaaaggcg
     4021 tatatttgcg ctgtgaatgg ttgacaaaag atgaaataga ataccttttg tcagctgaca
     4081 cttcctctta tcttattgat aaaatggatt tatgttccta cgtgcgcccc cagcccgcaa
     4141 caatgatcaa catgccgcaa agcgcaatca acgcacccgt ccagtcataa agagtcagtt
     4201 tcacgccatc cacaacgcgc agccacatca acgccgtgca gacataaacg ccaccataag
     4261 ccgcgtaaac acgcccactc gccgctggat gcaacgttaa caaccagaca aacagcgcca
     4321 gtgaaatccc cgccggaagc aacagccaga tactggcgtt tcgttttaac cacaaccagg
     4381 gcagaaagca tccaataatt tcacacagcg cagtagcaaa aaatagtaac gttgttttaa
     4441 tcatctttgt ctcttattga catcatgtat agttataggg cgacataata tcatcaatat
     4501 aaacaccctc ctggtacgtt ttgcgtccgc agtggatggt gtagaatcac ctttaatcat
     4561 tcatacaggg aatgaattat gaaaatcact ctcagcaaac gaatcggcct gctcgctatt
     4621 ctgctgcctt gcgcactggc attgagcaca actgttcatg ccgaaactaa caaactggtg
     4681 attgagtctg gcgacagtgc acaaagccgc cagcacgccg ctatggaaaa agagcaatgg
     4741 aatgacacgc gcaatctgcg ccagaaagtg aataaacgca ctgaaaaaga gtgggataaa
     4801 gccgacgccg cttttgataa ccgcgataaa tgtgagcaaa gcgccaacat caatgcctac
     4861 tgggagccca atactttgcg ctgcctggac cgtcgaactg gccgcgttat taccccctaa
     4921 cctgttattg atttaaggaa tgtaaggaca cgttatgcca agcgcccaca gtgttaagct
     4981 acgcccgctg gagcgtgaag atttacgcta tgaacatcaa ctcgacaaca acgccagtgt
     5041 gatgcgttac tggtttgagg aaccctacga agcctttgtt gaactctctg atctgtatga
     5101 taagcatatt cacgatcaga gcgaacggcg ctttgtggtg gaatgtgacg gcgaaaaagc
     5161 cggtctggtg gagctggtgg aaattaacca tgttcatcgc cgcgcagaat ttcagataat
     5221 tatctccccg gagtatcagg ggaaaggtct ggcaacccgt gccgccaaat tagcaatgga
     5281 ctatggcttt accgttctca atctctataa gctgtatctg atcgttgata aagagaatga
     5341 aaaagcgatt cacatttacc gcaagcttgg cttttcggtt gaaggtgaat tgatgcacga
     5401 gttctttatt aatggtcaat atcgtaatgc cattcgcatg tgtatattcc agcatcagta
     5461 tctggcagag cacaaaacac cgggtcagac tctcctgaag ccgaccgcac aatagcatta
     5521 ataataatcg atcgtatttt tgatggtgta aacccgttcg acggcgggtt ttactccttc
     5581 atcaacaatg attagctgac agtccaccgg attagcgtga ctgtcatatt cacagctctg
     5641 ttttacatta ccaacccgtt gattattcag taaagtaacc gctgtgtaat ctaatttttt
     5701 gatcggatcc gttgatggcg tggcgctgac agataatgtt ttgtcgttac ttttcgtggt
     5761 tttacccagc ggataaccct gatcatcata gcgatattcc atctgcattt gtttgctgct
     5821 ggctttaatc acgaagccat tatcatcggt ttcccaactc accccggcag aaggtaattc
     5881 tgctagctgg cattttccct gtaaacgtac tctcttctcc agcgtctcgg catcacggta
     5941 ataattggcg tccagtacca gagcgaccac ggtattattt tccagatcca gtaattcgag
     6001 tgaatcaaaa cagccttctt ccgacaaagt cccagaaaca cgtttcgtca cttcaccttg
     6061 ctcatccatt aatgtctgag tgaaatcttt taccggacca cgcagcggat caaaatcgaa
     6121 ttcattagag aaactggcca tctcaggggt aaatgaaagt gttacctctg tgcggtcaca
     6181 tcctgtgagg aatatcgcga gtaagcatgg tagtaatttg tatttcacaa cagtcaccag
     6241 agagtagaga tgattctcaa tcatagtagc aaatacagta ctttacacgt taaatgctat
     6301 gcttaaagaa gttatcttcg cgtaaggagc ttatgatgaa actctcaacc tgctgtgccg
     6361 cgcttctgct cgccctcgcc tcacccgtgg tactcgccgc gccgggatcc tgtgagcgca
     6421 tacagagcga tatatcacag cgcattatca ataatggcgt acccgaaagc agcttcacgt
     6481 taagtattgt acccaatgac caggttgatc agcctgattc ccaggtcgtc ggccattgcg
     6541 ctaatgatac gcataaaatt ctctataccc gcaccaccag cggtaacgtc tctgctcccg
     6601 cgcagtctac ccaggatggc gcgcctgccg aaccgcagta atacattatc gactgaacgc
     6661 cggatacgac acatccggcg atttgaacat acaacataat cccaccttat tactcatacc
     6721 cttctattga tatggattaa taattcttaa cccaaaatgg gtagactccc tctattgtta
     6781 gcgcgctaaa tattcaatat ataaacattt atataacgat aaagaacagg gagtgagtta
     6841 tgtccaaaaa tgaccgaatg gtgggcatca gccgcagaac actcgttaaa tcaaccgcga
     6901 taggttctct ggcgctggct gcaggcggtt tttctttacc gttcaccctg cgcagtgcag
     6961 cggcaacggt acaacaggcc agcgaaaaag tgatctgggg tgcctgttcc gtcaactgtg
     7021 gtagccgctg tgcacttcgt ctacatgtta aagataatga agtgacctgg gtggaaactg
     7081 acaataccgg cagcgatgag tacggcaacc atcaggtacg cgcctgtttg cgcggtcgct
     7141 ccatccgccg gcgtattaat catcccgatc gcttgaatta cccaatgaaa cgcgtgggta
     7201 cacgcggtga aggcaaattc gaacggatta gctgggatga agccctggat actatcgcca
     7261 gtagcctgaa aaaaaccgtc gaacaatatg gcaatgaggc tgtatatatt cagtactctt
     7321 cggggattgt tggcggcaat atgacccgct cttcgccatc agcctcggcg gtcaaacgcc
     7381 tgatgaactg ctacggcggt tcactcaatc agtatggctc ctacagcacc gcgcaaattt
     7441 cctgtgccat gccctacacc tacggcagta atgatggcaa cagcaccacg gatattgaaa
     7501 acagcaagct ggtcgtgatg tttggtaaca acccggcaga aacccgcatg agcggcggtg
     7561 gcatcactta tcttcttgaa aaagcgcgcg agaaatcgaa cgccaaaatg attgttatcg
     7621 atccgcgata taccgatacg gctgctggtc gtgaagatga atggctccct attcgcccgg
     7681 gcaccgatgc cgcgctggtt gcgggtattg cctgggtatt gattaacgaa aatctcgttg
     7741 atcaaccttt tctcgataaa tactgcgtcg gctatgacga aaaaacctta cccgcagatg
     7801 cacccaaaaa tggtcactat aaagcctata ttcttggtga aggtgatgat aacacagcga
     7861 aaacgccgca gtgggcttcg caaattaccg gtatcccggt agaccgtatc atcaaactgg
     7921 cgcgtgaaat tggcacagca aaacccgctt atatctgcca gggctggggg ccgcaacgcc
     7981 aggcaaacgg cgaactgact gcacgcgcta ttgctatgtt acctattttg acgggcaatg
     8041 tcggcatcag cggcggaaat agtggcgcgc gtgaatcgac ctataccatt accatagaac
     8101 gcctgccggt gctggataat ccggtcaaaa cgtcaatctc ctgcttcagc tggacagatg
     8161 ctatcgatca tggtccgcaa atgacggcaa tccgcgacgg cgtccgcggc aaagataaac
     8221 tggatgtgcc cattaagttc atctggaact acgcgggaaa taccctcgtt aatcagcatt
     8281 ctgacatcaa caaaacgcat gaaattctgc aggacgaatc gaaatgcgaa atgattgtga
     8341 tcatcgaaaa ctttatgacc tcatcggcaa aatatgccga cattctgctg ccagacctga
     8401 tgaccgttga gcaggaagat attattccta acgactacgc cggtaacatg ggatatctca
     8461 ttttcctcca gcctgtcacc agcgaaaaat tcgaacgcaa accgatttac tggatcctga
     8521 gtgaagtcgc gaaacgtctt ggacctgacg tctatcaaaa gttcacagaa ggtcgcacgc
     8581 aggaacaatg gttacaacat ctgtacgcca aaatgcttgc caaagatccg gcgttaccgt
     8641 cttacgacga actgaaaaaa tgggtatcta taagcgtaaa gatcccaatg gccattttgt
     8701 cgcctacaaa gcatttcgtg acgaccccga ggcaaatcca cttaaaacgc cttccggtaa
     8761 gattgaaatt tattccagca ggctggcgga aattgcccgt acctgggaac tggaaaaaga
     8821 tgaagtgata agcccattgc cagtttatgc ctcaaccttt gaaggctgga actcccctga
     8881 acgtagaacc ttcccactgc aactgtttgg tttccattac aaatcccgta ctcactcgac
     8941 ctacggcaat attgatctcc tgaaggctgc ctgccgtcag gaggtgtgga tcaaccctat
     9001 agatgcgcag aaacgtggga ttgccaacgg cgatatggtg cgggtgttta accatcgtgg
     9061 cgaagttcgg ctaccagcca aagtaacacc acgtattctc cctggagtta gcgctatggg
     9121 ccagggagcc tggcacgagg ccaatatgtc tggcgataaa atcgaccatg gcggctgtgt
     9181 gaatacgcta accactctgc gcccttcacc actggcgaag ggaaacccgc agcacactaa
     9241 tctggtcgag atcgaaaaaa tataacccac gacaaccata aattctggca tgacattttg
     9301 tttgaaaagc aataagtgag taatgatgaa aatccatacc acagaggcgc tcatgaaggc
     9361 tgaaatcagc cgtagaagtc tgatgaaaac ctccgcactt ggcagtcttg cgctggcaag
     9421 cagtgctttc actctgccat tttcccaaat ggtccgggcg gcagaggctc cggtagaaga
     9481 gaaagcggtc tggagttcct gcaccgttaa ctgcgggagc cgctgtctgt tacgtttgca
     9541 tgtgaaagat gacaccgtgt actgggtgga gtctgatacg acaggtgacg acgtctacgg
     9601 taaccatcag gttcgagcgt gtttacgcgg gcgctctatc cgccgacgga tgaatcatcc
     9661 tgataggttg aaatatccca tgaagcgcgt cggcaagcgc ggtgaaggtt aatttgaacg
     9721 gataagttgg gacgaagccc tggataccat cagtgataat cttcggcgga tcctgaaaga
     9781 ttacggcaat gaggctgtac atgtcctgta cggaacaggc gtagatggcg gaaacatcac
     9841 caactcaaac gtcccgtacc gtctgatgaa ctcttgcggt ggttttctca gtcgctatgg
     9901 cagctacagt accgcacaga tcagtgccgc aatgagttat atgttcggtg ccaatgatgg
     9961 caacagcccg gatgatatcg ccaatacgaa actggtcgtt atgttcggaa ataacccggc
    10021 agaaacgcgg atgagcggcg gtggtgtcac ttactacgtc gagcaagccc gcgaacgttc
    10081 aaacgcacgc atgatcgtca tcgatccacg ttataacgac actgctgccg ggcgtgaaga
    10141 tgaatggctg cccattcgcc ctggcaccga tggcgcactg gcctgtgcga ttgcctgggt
    10201 actgattact gaaaacatgg tcgatcagcc atttctcgac aaatattgtg ttggttacga
    10261 tgaaaaaacg ctgcccgcca acgcaccacg taacgcgcat tataaagcct atattctggg
    10321 cgaagggcct gacggcatag ctaaaacgcc ggaatgggca gcaaaaatca ccagcatccc
    10381 ggcagaaaaa attatccagt tggcacgaga gatcggttca gcaaaacctg cttatatttg
    10441 tcagggttgg gggccacaac gacattccaa cggcgagcaa acgtcccgcg ctattgccat
    10501 gctttccgtt ctcaccggta acgtcggcat aaacggcggc aactcaggtg tacgcgaagg
    10561 tagctgggat ctgggggtag aatggttccc gatgcttgag aatcctgtta aaacgcagat
    10621 ttccgtcttt acatggacag atgccatcga ccatggtacg gaaatgaccg cgacccgtga
    10681 tggtgttcgt ggaaaagaaa aactggatgt ccccatcaag tttttatggt gctacgccag
    10741 taacacattg atcaatcaac atggcgacat caatcacacc catgaggtgc ttcaggatga
    10801 cagcaagtgc gagatgattg ttggcattga ccacttcatg acggcctcgg ctaagtattg
    10861 cgatatcctg ttgcccgacc tgatgccaac agagcaagaa gaccttatct ctcatgaatc
    10921 tgcagggaat atgggctatg tgatcctcgc ccaacccgca acctcagcaa aatttgaacg
    10981 caaacccatc tactggatgc tgagtgaagt cgccaaacgc ttaggaccag acgtttatca
    11041 aacctttact gaaggtcgca gtcagcatga atgggtcaaa tatctccatg cgaaaacgaa
    11101 ggaacgtaac cctgagatgc ccgactacga ggagatgaaa acgaccggga tctttaagaa
    11161 aaaatgcccg gaagaacact acgtcgcttt ccgcgcattc cgtgaagatc cacaggcaaa
    11221 cccgttgaaa acaccttcgg ggaaaatcga aatttattct gaacgactgg cgaagattgc
    11281 agatacctgg gaattgaaaa aagatgaaat tattcatccc cttcctgcgt ataccccagg
    11341 ttttgatggc tgggacgatc ccctgcggaa aacctatcca ctgcagttaa cgggcttcca
    11401 ttacaaagcg cgtacccact ccagctacgg caatattgat gtgttacagc aggcctgccc
    11461 acaagaggtg tggatcaacc ccattgatgc tcaggcacgc ggtatccgtc atggcgatac
    11521 cgtgcgggta tttaacaata atggagaaat gctgattgcc gcaaaagtga ctccgcgtat
    11581 tctgcctggc gttaccgcca tcgggcaagg tgcgtggctt aaagcggata tgtttggtga
    11641 ccgggtcgat cacggcggca gtatcaatat tctgacctct caccgccctt caccgctggc
    11701 aaagggaaac ccgtcgcaca gcaatcttgt ccagatcgaa aaggtttaag gagtagccca
    11761 tgaccacaca atatggattt tttatcgatt ccagccgctg tactggctgt aaaacctgcg
    11821 aactggcgtg caaagatttt aaagatcttg gcccggaagt cagtttccgc cgtatttatg
    11881 aatacgctgg cggcgactgg caggaagata atggcgtctg gcaccagaac gtgttcgctt
    11941 attatctctc catctcctgt aaccattgcg atgaccccgc atgtacaaaa gtctgcccca
    12001 gcggcgcaat gcataagcgt gaagatggct ttgtggtggt tgatgaagat gtttgtatcg
    12061 gctgccgcta ctgccacatg gcttgtccgt acggcgcacc acagtacaat gctgaaaaag
    12121 ggcacatgac gaagtgcgat ggttgttatt cgcgcgtcgc cgaggggaaa caacccatat
    12181 gcgtcgaatc ctgcccgctg cgtgcacttg agttcggtcc aattgaagag ctgcgccaga
    12241 agcacggcac tctggctgcc gtcgcgccac tgccgcgcgc gcatttcaca aaacccaata
    12301 tcgttatcaa acctaacgcc aacagccgcc cgaccggcga taccactggt tatctggcta
    12361 atccggagga agtgtaaaat gggaaatgga tggcatgaat ggccactggt tatctttacc
    12421 gtattgggtc aatgcgtagt aggcgcgctg attgttagcg gaattggctg gttcgccgcc
    12481 aaaaatgacg cagaccgtca gcgtattgtc cgtggcatgt ttttcctctg gctgttgatg
    12541 ggcatcgggt ttattgcctc tgtcatgcac ctgggatcgc cactgcgggc gtttaactcg
    12601 ctgaatcgaa tcggagcttc tggtctgagt aacgagattg ctgccggttc catatttttt
    12661 gccgttggcg gactatggtg gctggtggcg gtcatcggta aaatgccgca agcgttgggg
    12721 aaactctggt tacttgtcag catggcgctc ggcgtcattt tcgtctggat gatgacctgc
    12781 gtgtatcaaa tcgacactgt gccaacctgg cataacggtt acactacgct ggctttcttc
    12841 ctgacggtat tgttgagcgg tccaatactt gctgccgcaa ttctgcgggc agcccgcgtt
    12901 acatttaata ccacaccatt tgccatcatt agcgttctgg cgctgatcgc ttgtgccggg
    12961 gtgattgtgc tgcaaggttt gtctctggcg tctattcact catccgtgca acaagccagc
    13021 gcactggtac cagattacgc ctccttacag gtctggcgtg tggtattgtt atgcgcaggt
    13081 cttggatgct ggctttgccc gctcattcgt cgccgtgaac ctcacgttgc cggacttatt
    13141 ctggggctaa tcctcatact tggcggtgag atgatcgggc gtgtgctctt ttatggcctg
    13201 cacatgacag taggaatggc tattgcaggt taacaaattg gtgcggggca acccgcacat
    13261 ttcaggatgt taggaatgac ccatttttca cagcaagata atttttctgt cgcggcgcgc
    13321 gtgttgggcg cgctgtttta ttacgctcca gagagtgcgg aagccgcacc tctggttgcg
    13381 gtactcacca gtgacggctg ggaaactcag tggcctctac cagaagcgtc attagcgccg
    13441 ctggtgacgg cttttcagac acagtgtgaa gagactcacg cccaggcctg gcagcgtttg
    13501 tttgtcggcc cgtgggcact gccgtctccg ccgtgggggt cggtctggct ggatcgcgaa
    13561 tctgtgctgt ttggcgattc aacattggca cttcgtcagt ggatgcgcga gaaaggcatt
    13621 cagtttgaaa tgaagcaaaa cgaaccggaa gatcattttg gttcactgct gttgatggct
    13681 gcgtggttgg cggagaatgg tcgccagaca gaatgcgaag aattgctggc atggcacctt
    13741 tttccgtggt caacacgttt tcttgatgtt tttatcgaaa aagcagaaca ccctttctac
    13801 cgtgcactgg gtgaactggc tcgcttaacg ctggcgcaat ggcaatcaca actgttaatt
    13861 cctgtcgcgg ttaaaccgct gtttcgatag gatcaccgta atattgccgg atggtgacga
    13921 ttacgcgcaa tccggcaata ataggttaca gtgtcacgtt tttttatctc ttaaagcacg
    13981 cactgctttt gcggctggcc tcttttgccg caaaatagtc gcccgtgttt cattgcccat
    14041 ttctgctcat gcatcatcta cacatctatc cggatctgcg cactatgttc cgccgtctgc
    14101 ttatcgcaac agtcgtcggt attctcgcgg cctttgccgt tgccgggttt cgtcatgcga
    14161 tgctgctact ggagtggttg ttcctcaata atgactccgg cagtctggtc aatgcagcga
    14221 caaacctttc cccctggcga cggttgctaa ctccggcgct cggcggactg gcggcgggtt
    14281 tgttgctgat gggctggcag aaatttaccc aacaacgccc tcatgcgccg accgattaca
    14341 tggaagcgtt gcaaaccgat ggacagttcg attacgcagc aagcctggtt aaatcgcttg
    14401 cctctctgct ggtagtaacc agcggcagtg caattggtcg cgaaggtgcg atgattcttt
    14461 tagctgccct tgccgcctcc tgttttgccc aacgttttac gccacgccag gagtggaaat
    14521 tatggatcgc ctgtggggcc gcggcgggaa tggctgcggc ctatcgtgcc ccgcttgctg
    14581 gcagtttatt tatagccgaa gtgctgtttg gcactatgat gttggcctct ctcggcccgg
    14641 tgattatttc cgccgtcgtg gcattgctgg ttagcaatct gattaatcat agcgacgcgt
    14701 tactctacaa cgtacaactc tcagtgacgg ttcaggctcg tgactatgcg ctgattatca
    14761 gtacaggtgt gctggcaggt ctgtgcggac cactgttgtt aacgttaatg aacgcctgtc
    14821 atcgtggatt tgtgagtctc aaacttgcgc cgccctggca actggcacta ggcgggttga
    14881 tcgtgggtct gctttccctg ttcacacctg cagtgtgggg caacggctat agcaccgtac
    14941 aatccttttt aaccgcccca ccactgttaa tgatcattgc cgggatcttc ctttgtaaac
    15001 tgtgtgccgt gctggcgagt agcggttccg gcgcacccgg tggtgtcttt acaccgacgc
    15061 tatttatcgg tcttgccatt ggcatgttgt atggtcgtag cctgggatta tggttccctg
    15121 atggcgaaga aattacgctt ttactcggat tgaccgggat ggcgacactg ttggcggcaa
    15181 ccacgcacgc gccgattatg tcgacgttga tgatatgtga aatgaccggg gagtatcagc
    15241 tactccccgg tttattgatt gcctgcgtca ttgcgtcggt aatttcgcgg acgttacacc
    15301 gtgactctat ctaccgccag cacactgcgc agcatagcta agcggatgta ttgccccagt
    15361 tctcgctgtt cggcgcgcgg cagataaggc agttcaccaa tgagcggtgc cggaagtttt
    15421 ttacccagca catcaatgat ttccgcataa tgcgccagtc ctgggttgat tcggttagcc
    15481 acccagccaa tgagcggcag cccgtcgttg gcaatcgcct gagctgttag cagtgcatgg
    15541 ttaatgcaac cttcctgaat accgacaacc atcaacaccg gcaactgttc ctgcaccacc
    15601 cattcagaga gtggacgcaa atcattcatc agactgcgcc agccgccagt cccttctacc
    15661 acgacatgat cgactttatc ggtcaggttt gccaggccgt ttgaaatgag ggtgtaattg
    15721 attgggcaac tgtgcgccac gctactttct tcttcgctta acgcgatagg attaactgct
    15781 tcataaggca gttcgatggt tgaaacactc tgcaacacca gggcatcttt attacgcagc
    15841 ccttcgggtg tctctttgct ccccttcgct acgggtttat atcccgcaac cgtttttccc
    15901 tgggaggcta acgcttgtag caatgcgcgg gaaaccaccg ttttccctac agaagtgtct
    15961 gtaccggtaa taaagaaacg cttcagcatc actaactcca ccgttatgct tcacaaatat
    16021 aaaccaggaa aataattaac cttgaaagtc taagttatgc tttcctggcc caaattgaga
    16081 tagcgcaaat tttggtagaa cagttaaaaa atgttaaccc tgcaacagac gaatcaacaa
    16141 agaaccgtta tacatcgcgt cttttaccag tgcagcgcct gccatcgtgc cctggttaga
    16201 aaactgagta ctctcaacgc tgatgtgctg actatacgca ggaagggcct gctgacggat
    16261 gctgtctgag atgaccggga agaggatatc tgccgcttta cttaacggtg agccaatcag
    16321 tattttttgt gggttaaata aattcaccat gatggcaaga atgcgcccta catgcgcgcc
    16381 caccccggta atgatgtctt ttgccagcag atcgccgcgc aatgccgcct gacacaatga
    16441 gtccacggtt aacggttgtc catgtaacat cgagctcatg gattgattaa gacgcagctg
    16501 tgccagctca agaatactgt ccacactggc gatggtttcg aggcagccgt gattcccgca
    16561 ataacagcgt ttcccatacg ggtcgacctg tgtgtggcct atttccacga gactactgct
    16621 gcctgcgtgt agcagatgac catcggtaat gacgcccgcc cccacgttgt gatcgataac
    16681 cacctgaatc acatcgcgcg ccccgcgtga ggcaccaaac aaggcctctg ccatcgtcca
    16741 tgcgctgata tcatgctgaa tataaaccgg tacgccggta tgctgctcca gcgcctcgcc
    16801 gagcggcatc tcttttacat cctcgtagaa cggcatgcga tgtacaatac cattttccgt
    16861 atcaataatt cccggcaagg ttatggcaat cgaagttaga cgctcaagtt ttttctggtg
    16921 gcgaataaaa aactgatcga tatgggaaat gatacgatcc agcaatggcg agtcatcttt
    16981 taacgccagt tcctgcgact cttccaccac cagtttgctg ctcagatcgc gcagagcaag
    17041 gaaaatctcc ccgcgactaa tgcgcagaga aagatagtgc caggcttcag tttcaaccac
    17101 cagccccacc gccggacggc cacggttccc cgcttctttg atttccagct cttgcaccag
    17161 gtgtgcttcg agcatctcac ggacaatttt agtgatactg gcaggagcca gctgcgccag
    17221 acgggaaaga tcgatacgcg agactggacc aagctgatca atcaggcgat aaaccgcgcc
    17281 cgcgttggtc tgctttattt gatcaatgtg cccaggctgg ttttcagcaa ccaccgcata
    17341 ctccctatat tttcgcgctc cgaaataatc tgtaggctat ggtgaagcac ttcaatacgt
    17401 gtcgtcaaat ttttacttag gcatgtgatt aacagcacat ttttcgggct ttttcgctga
    17461 aatttccctc acctgagagc attcagcaga tgaatacgaa agttacgcgc agccggactt
    17521 tgttcatgat gtttcggcca gaccaaccac atttcagaaa ccgcatcctc ttcagcaatc
    17581 ggcacccagc gcatttcgtt gagctgaaca cgtttaaatg aagcaggcaa aattgaaaca
    17641 cccagaccgg cggaaaccag accgatgatg gtcattgcct cgcccacctc ctgagtgatg
    17701 acgggcgtca aatggtaacg tcgcatcagc ccgagaatat cgtcatacag ccctgtcccg
    17761 acgtgcggat caaaaaagac gaagggttct ttcgccagtt cagccagcgt tacattcggg
    17821 ttatttgcca ggggatgatc gtgcgggatc atcgccataa gcggttcatg gacgatgact
    17881 gcgtgttcaa gcgtctccgg taacgctgtg taacgcagca atcccatatc cagcgttcct
    17941 tcaatgagcg gagcgatttg ctcacgagtg ttcatttcgc gggtttgtaa atggacatcg
    18001 ggataatcac ggcgaaacag cgataacgta tcggacaccg cccgaataaa aggagcggac
    18061 gaagtaaaac caatgcgcaa ctcccccgct tccccctgat gcagtctttc agcgcgagcg
    18121 gcagcgtcat ctaccataga caggatttgc cgactatctg caagaaactg ttttcctgct
    18181 gccgtcagca atacactgcg attagttcgt gccagcagtc gggcaccaat ttgttgctcc
    18241 agtgcctgaa tctgctgact tagcggtggt tgcgaaatat tcaggcgggc agcggcgcgc
    18301 ccgaaatgca gctcttccgc aacagcaaca aagtaacgca
//
LOCUS       DAZGOB010000093        18320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGOB010000093 DAZGOB010000000
VERSION     DAZGOB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.093"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            448..789
                     /gene="symE"
                     /locus_tag="ACHLIF_003434"
     CDS             448..789
                     /gene="symE"
                     /locus_tag="ACHLIF_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO9607391.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVITGKQKVA"
     gene            complement(836..1999)
                     /locus_tag="ACHLIF_003435"
     CDS             complement(836..1999)
                     /locus_tag="ACHLIF_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO9607392.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLSEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(2047..2929)
                     /locus_tag="ACHLIF_003436"
                     /pseudo
     CDS             complement(2047..2929)
                     /locus_tag="ACHLIF_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(3026..3190)
                     /gene="yjiS"
                     /locus_tag="ACHLIF_003437"
     CDS             complement(3026..3190)
                     /gene="yjiS"
                     /locus_tag="ACHLIF_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO9607393.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            3367..4779
                     /gene="yjiR"
                     /locus_tag="ACHLIF_003438"
     CDS             3367..4779
                     /gene="yjiR"
                     /locus_tag="ACHLIF_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO9607394.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSREHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWGAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQNYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(5021..5965)
                     /locus_tag="ACHLIF_003439"
     CDS             complement(5021..5965)
                     /locus_tag="ACHLIF_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO9607395.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIYLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVNTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRALPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            6432..7664
                     /gene="mdtM"
                     /locus_tag="ACHLIF_003440"
     CDS             6432..7664
                     /gene="mdtM"
                     /locus_tag="ACHLIF_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO9607396.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVHQHQAAELAEER"
     gene            7705..8985
                     /gene="yjiN"
                     /locus_tag="ACHLIF_003441"
     CDS             7705..8985
                     /gene="yjiN"
                     /locus_tag="ACHLIF_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO9607397.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            9101..10252
                     /gene="yjiM"
                     /locus_tag="ACHLIF_003442"
     CDS             9101..10252
                     /gene="yjiM"
                     /locus_tag="ACHLIF_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO9607398.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            10262..11029
                     /gene="yjiL"
                     /locus_tag="ACHLIF_003443"
     CDS             10262..11029
                     /gene="yjiL"
                     /locus_tag="ACHLIF_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO9607399.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            11026..11283
                     /gene="ytiA"
                     /locus_tag="ACHLIF_003444"
     CDS             11026..11283
                     /gene="ytiA"
                     /locus_tag="ACHLIF_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO9607400.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEMQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            11348..12208
                     /gene="yjiK"
                     /locus_tag="ACHLIF_003445"
                     /pseudo
     CDS             11348..12208
                     /gene="yjiK"
                     /locus_tag="ACHLIF_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
     gene            12276..13454
                     /gene="yjiJ"
                     /locus_tag="ACHLIF_003446"
     CDS             12276..13454
                     /gene="yjiJ"
                     /locus_tag="ACHLIF_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9607401.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(13467..14021)
                     /gene="kptA"
                     /locus_tag="ACHLIF_003447"
     CDS             complement(13467..14021)
                     /gene="kptA"
                     /locus_tag="ACHLIF_003447"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO9607402.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRTLLDTVVATSDKKRFSYSSDGRCIRAVQGHLTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQTENGVWLTSTVAVEFLEW"
     gene            14271..14954
                     /gene="yjiH"
                     /locus_tag="ACHLIF_003448"
     CDS             14271..14954
                     /gene="yjiH"
                     /locus_tag="ACHLIF_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO9607403.1"
                     /translation="MTQQGDAVAGDLATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            14951..15412
                     /gene="yjiG"
                     /locus_tag="ACHLIF_003449"
     CDS             14951..15412
                     /gene="yjiG"
                     /locus_tag="ACHLIF_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO9607404.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            15425..16597
                     /gene="iadA"
                     /locus_tag="ACHLIF_003450"
     CDS             15425..16597
                     /gene="iadA"
                     /locus_tag="ACHLIF_003450"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO9607405.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILFPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            16662..17574
                     /gene="hypT"
                     /locus_tag="ACHLIF_003451"
                     /gene_synonym="qseD"
                     /pseudo
     CDS             16662..17574
                     /gene="hypT"
                     /locus_tag="ACHLIF_003451"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
     gene            complement(17567..17959)
                     /gene="iraD"
                     /locus_tag="ACHLIF_003452"
     CDS             complement(17567..17959)
                     /gene="iraD"
                     /locus_tag="ACHLIF_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO9607406.1"
                     /translation="MMRQSRQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSSLPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(17956..18039)
                     /gene="idlP"
                     /locus_tag="ACHLIF_003453"
     CDS             complement(17956..18039)
                     /gene="idlP"
                     /locus_tag="ACHLIF_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO9607407.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(18155..18298)
                     /gene="ytiD"
                     /locus_tag="ACHLIF_003454"
     CDS             complement(18155..18298)
                     /gene="ytiD"
                     /locus_tag="ACHLIF_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO9607408.1"
                     /translation="MADYAEINNFTPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         4323 a         4632 c         4537 g         4828 t
ORIGIN      
        1 gctcacagat ttaccgcttt gataatggct cagcacaacc caacctatca gcaaatagtg
       61 taatgttgta ttcattccca tgcccaccat taactgaaca atatagaata ttttcacaag
      121 taggtttact tcatgaacta tgcgataagc ttaaaactcg tataaaaaca gcccaacaaa
      181 cccaactgca ccttgcagac gcactcactg acgcggcgat aaactaattt tctcgccctc
      241 aaaaaaccaa taaagcggct aaatggccgc tttattcaca tcaccaaaaa ttatatttca
      301 cgctaatttt catcttattt tcattaattt ttagctggac tttccctgta tttactgatg
      361 atatatccag gtatttagcg cggtgcggat gtgcgccaac acacccgcac cgctaatcac
      421 aatcactatt atcggagaat agcagttatg actgacacgc attctattgc acaaccgttc
      481 gaagcagaag tctccccggc aaataaccgt caattaaccg tgagttatgc gagtcgctac
      541 ccggattaca gccgtattcc cgccatcacc ctgaaaggtc agtggctgga agccgccggt
      601 tttgccaccg gcacggcggt agatgtcaaa gtaatggaag gctgtattgt cctcaccgcc
      661 caaccaccca ccgccgaaga gagcgaactg atgcagtcgc tgcgccaggt gtgcaagctg
      721 tcggcgcgta aacaaaagca ggtgcaggat tttattgggg taattaccgg taaacagaaa
      781 gtcgcctgat aaattgaccc ggtagcgcca gtgctaccgg gtattattcg ccgcgttata
      841 ccaacgcgct ctccatctgt ttttcaaatg cctgacgaat taatcccata atgtaaggaa
      901 gttgtgcgag gtcgctgaaa ccaatttcta catcgccatt accccagcgc ccaacatttg
      961 tcacatcttt ggcaatacct ttcggatcga ccagttcatg aaactgcata ttaagcgaca
     1021 accgcagtcg gcttttttgc ggtacaacat caacaaaatt cgtttcagct ttaaacgcaa
     1081 tatagagctt caatacttcc tgcgtgatcc ctgcgtccag gcgcataact tcatcgcgaa
     1141 gatgatcgaa taacacccgg ctatgcgaac catcagcaag aaaaggataa tcactcaggc
     1201 tgtaactggt tttgttttca ggctgccccc gataaacagc taaaacctct tcagaaagag
     1261 aaggcgcttg ccagacctga accgcgagat cggccagacg atcggcacgg gcgcgaatgg
     1321 cggcttcatc ccatttttcg cactgcccaa gaccgatatt caaatagagc gggctatgtt
     1381 taaagccgtc ttcaatatcg cgtttttccg cgaaaggtct gtcactgtag cgggagttat
     1441 agcgcgttaa agtgagattc cccaacgtat gtaacagttc tttatgcaca cgctgccagt
     1501 cgcttcccag ctcttcacgc catttagccg acagattttc gttctgcggc ataatgtgct
     1561 caatcgtaaa ctcttccacg cgctctctgc gtttatcgtt ttccagtctt cgtaaccagt
     1621 agctgcggct gcggaagtta tagagatcgc gaacttttaa ttcccgttta aattcatcat
     1681 cgttggggaa acgacgatat gaaggcaggt tcataaaatg tacctgaata ctttccagat
     1741 atttttcttt attaatgacc ttataaaaag tggcaaacgt cttgttcaaa gaattcgtcg
     1801 gaattgcaca tactgcacgg cggaaaacat aagattcaat taaacgaatt atgctcagga
     1861 aatcttcgtg agacaaatcg tcatttttat agtcatgata aagcgccagt aagaaaggat
     1921 acgccacatc aacctttaac tcgcgcaaat cctgaaaagc cgtagcaagc gatttgtcac
     1981 tttcttttcc caatgccatt gcacagtaat actccgcgta gatatgaata tcttcaacca
     2041 gccgatctac gcctttttca gcaacactct ggctgcgggc atggagttta aatgcctcat
     2101 agacttcatc tgtccgaggg atctcccccg tttttaccgt cagataatga cgcataaaac
     2161 tgtcaaaata ttcgctgtaa ccttgctgac caaaggcgac ttccattgga cgccagtgat
     2221 cttcatacaa ccgggtttga tgctctggtt cgaggcccat cagaataaaa ttgcgcacca
     2281 gatcggcctg acttaacgcc ttaccggtgg agttcatact ttcaaaaatc agttgcggat
     2341 tatcctgacc acgattaagc gccacatcga caattaataa ctttgctaac ccacgacata
     2401 aggggatcaa gtcatcaccg agtttggcaa tttgttcatc aaagaaagta aagttttcca
     2461 ttattcgatg ggaatagttt tctggcattg gtctttgttt tattaacgcc agtaaactgt
     2521 ctttatcagt ctcggtcagt agtaatttaa aacctttctc gccggactca taaggattga
     2581 gcaaataata attacgcaat ttcattgctg aaaagccatc aaaaacttcg tcttcgccaa
     2641 gatggcgcga taaagcctca atcagcaaca ttgcggtcgt cagccgttgt tgaccatcaa
     2701 tgaccagtaa cggagaaata ccagaaacct gatacaaccc ctgctcaata taaacaaccg
     2761 aaccgataaa atgcgctgat atatcgtcac gctttccggc acgaatgatg tcgtcccaaa
     2821 gttgccgaca ttgttgttct gtccatgaat aagtccgttg ataaatggga ataacaaact
     2881 gctgcgaacg ttttaaaaaa tcgagtaggc gggcttctat agctttcatt ctgacgtctt
     2941 tccgtgaagc gatgatttcg caataataac cacttccttt tggaggttat agagaaatcc
     3001 tgtttatccg cgatgaattg ccccctcact ccacatcctc cctgcgtaac ccaatatctc
     3061 tcaaccgctc atcactcatc tgctgtaaca ctcgtcgggt ctgcatttgc cgccgccaac
     3121 gcctcaccgc ctgccagagt tgcacaagtc tgataaacgg cgttttggct ttgttttcgt
     3181 gaaattccat ctgctgcctc ctcatctttc agaggcttca tcatccacct tctggcagtt
     3241 cacaatacag atgcaaatat tagttttatt taacatacag acaggttaaa acagcatctg
     3301 catccggttt tttagcctca tctgtaccgg tttttctttc tgtatggtcc aaataaggaa
     3361 tacagcatga cgcgttatca acatctggcg actctacttg ccgagcggat tgagcaaggg
     3421 ctgtatcgtc atggggagaa attgccgtcg gtgcgtagct taagtcggga gcacggcgtc
     3481 agcatcagca ccgtgcagca ggcgtatcag acgctggaga cgatgaagct cataacccca
     3541 cagccgcgtt cgggttattt tgtcgcacaa cgtaaagccc agccgccagt gccgccgatg
     3601 acgcgtccgg tgcagcgccc ggtggaaatt acccagtggg atcaggtgct ggatatgctg
     3661 gtggcgcata gcgacagttc cattgttccg ttaagcaaaa gcacgccgga tgtcgaaacg
     3721 cccagcctga aaccgctctg gcgggagcta agccgggtgg tgcagcataa tctgcaaacc
     3781 gtgctcggtt atgacttgtt agccggtcag cgggtattga gagagcagat tgcccgcctg
     3841 atgctcgaca gcggctcggt ggtcaccgcc gatgacatca ttatcaccag cggctgccat
     3901 aactcgatgt cgctggcgtt aatggcggtg tgtaaaccgg gcgatattgt cgcggtcgaa
     3961 tctccctgtt attacggttc gatgcagatg ctgcgcggca tgggcgtgaa agtgattgaa
     4021 atcccaaccg atccagaaac aggcatcagc gttgaagcgc tggaactggc gctggaacag
     4081 tggccgatta aaggcatcat tctggtgcca aactgtaata atccgctggg atttattatg
     4141 ccggacgcac gcaaacgggc cgttctctct ctcgctcagc gtcatgatat tgtgattttt
     4201 gaagatgatg tctacggcga actggcaacg gagtatccgc gcccgcggac catccattca
     4261 tgggatatcg acgggcgagt gctgttgtgc agctcgttca gtaaaagtat tgctccaggc
     4321 ctgcgcgtgg gttggggcgc accggggcgt tatcacgata aactgatgca tatgaaatac
     4381 gccatcagca gctttaatgt gccgtccacg caaatggcgg cggcaacgtt tgtgctggaa
     4441 ggtcactatc atcgccatat ccggcggatg cggcagaact atcagcgcaa tttggcgctt
     4501 tatacctgct ggatacggga atattttccc tgcgaaatct gtattacgcg cccgaaaggc
     4561 ggatttttac tgtggataga attgcctgaa caggtcgata tggtctgcgt tgcgcggcag
     4621 ctgtgccgca tgaatatcca ggtggcagca ggctcgattt tctcggcttc cggcaaatac
     4681 cgtaattgtc tacgcatcaa ctgcgctttg ccgctcagcg aaacctatcg cgaagcacta
     4741 aagcaaattg gcgaggccgt gtatcgggca atggaataaa ttcgaattag tggcgaagca
     4801 cctgatttcc tctcacgaat cgcctgtaac tcgttgtatc tttgcagggt catcgccacg
     4861 ttccagcatg gcttaggcaa agtgtgcttc gttcataccg gatgcggcgt gaacgcctta
     4921 tccggcctac aaaccgcgca aattcaatag attgcagagg aaatgtaggt ctgataagcg
     4981 tagcgcatca ggcagctttg cgtttgtcat ccgtctctga ttagtattcg atccatggat
     5041 cgcgcttgat ttcaggcaag ggctgctcta atgcctgcat ttgctctgtc gaaagtccgg
     5101 taatcttttg tatccattca ggatctgccc cattctgcaa caacaatcta aggaggcgtt
     5161 gttccccttc ttctttcccc ttttccattc ccaacagcca tccatcatta taaattcgct
     5221 ctgcaatcgt cattattcgc tccctgtgtt gtggcattcg acgggtaagt tcgctgataa
     5281 acttcttaaa acgcgcttca tcgccagtca gtaaaatgta atttaacagc gcagttatct
     5341 ggctgtcatt agcacactct gtaaccagta atactaccag ttggtcgata agccccatca
     5401 gatcgcgctg gcgaatatgc ttttggatca actccaacag ggcgactctg cgatgctgca
     5461 caatctcgtc gtctggcacg acagtgatat ccaccagcgg gaacgcggcg gtataaagtt
     5521 tccgtgcggt agttgggtcg gcaaattcgt ccagccagca cagggaccag ggataaggac
     5581 tacggctacc gtgataaaat agcatcggta taaccagcgg tagcgcccga cgtttatcat
     5641 gctcgatatg gcgctgcatc accgccatgg aatagcgcat caggcgaaag gccatatgaa
     5701 tatcctcacg gctctgatgt tcaatcacca cataaatata gccatcgcct tcgcgggtat
     5761 ttaccgacca cagaatatcg gagtgtagcg cccgcaattt ttcatcgaca aagctggcgg
     5821 attccagttt taagctgtcg agatcgcaca gttcacgtaa atctttgggt aaatagatct
     5881 ccataaaatc ccgcgcggtg tcagggtggg tgagaaagga tttaaataat gcgtcatgcg
     5941 gcgtgctggt tgtgaagttt gtcatggcga tccgtcacct gttgataaga tggcccgact
     6001 ttagcgacta acaaaaccat tcacacctgg caatttcccg ttccccgagg cgctttccag
     6061 gctatttttg ttcctctgca agcgatacat ttttccggaa acaggctcgc gttttacatt
     6121 tcctgtaccc ggtagatatt cccctttcca ccgcgctctt ttgattaatc gcaaatttta
     6181 attacactta aggtgtatat tttctatgca aaccatcaat tcaagaggtg taatgtgctg
     6241 atgactattt gtaatcgtta tacattctga cccgaactca gaaggtattt ctctgtctgt
     6301 ataagcacag gcagtgtggt tgattacatg aaattcagta catttgtcct tctcgcctac
     6361 ctttcgtcat taccgacggt attgaatttc gttttccccg ttggggttct ccggacaagg
     6421 agttgtttgt tatgccacgt ttttttgccc gccatgccgc cacgctgttt ttcccgatgg
     6481 cgttgatttt gtatgatttt gccgcgtacc tgtcgacgga cctgattcag ccggggatca
     6541 ttaatgtggt ccgtgatttt aatgccgatg tcagtctggc cccggcagcc gtcagtctct
     6601 atctcgcggg cggtatggcg ttacagtggc tgctggggcc gctttccgac aggattggcc
     6661 gcaggccggt gctgattacc ggggcgctaa tatttaccct tgcctgcgct gcgacaatgt
     6721 tcacaacgtc tatgacacag tttcttatcg cgcgtgcaat tcagggcacc agtatctgtt
     6781 ttattgctac cgttggttat gtcacggtgc aggaggcgtt cggacagaca aaagggatca
     6841 agttgatggc gattatcacc tccatcgtac tgattgcgcc gattattggc ccgctttccg
     6901 gcgcagctct gatgcacttt gtgcactgga aagtcctttt tgccatcatt gcggttatgg
     6961 gttttatctc atttgttggc ttactgctgg cgatgccaga gacggtgaag cgcggcgcgg
     7021 ttccatttag cgccaaaagc gtcttgcgcg attttcgtaa tgtcttttgc aatcggctgt
     7081 tcctctttgg cgcagcaacc atctctttaa gctatatccc aatgatgagt tgggtggctg
     7141 tctcgccggt gatcctgatt gacgcaggcg gcttaacaac ttcgcagttc gcctggacac
     7201 aggttccggt gttcggcgcg gtgattgtcg cgaatgccat cgtggcgcgt ttcgttaaag
     7261 atccgaccga accgcgcttt atctggcgtg ccgtacccat tcaactggtc ggcctcgcac
     7321 tgttgattat cggcaatctg ctgtcaccgc acgtctggct gtggtcggtg ctgggcacca
     7381 gtctgtatgc tttcgggatt ggtttaatct tcccgacctt attccgcttt acactttttt
     7441 ccaataactt accgaaaggg accgtctccg catcgctgaa tatggtgatc ctgatggtga
     7501 tgtctgtctc ggtcgaaatc ggccgctggc tgtggtttaa cggcggtcgc ttgccgtttc
     7561 atctgttagc cgtcgtagcg ggagttatcg tcgttttcac cctggcgggg ttgctcaatc
     7621 gcgtgcacca acatcaggct gccgagctgg cggaggagcg gtgatttttg cgcgatcctg
     7681 ccgtcaggct ctattcttaa cgttatgaat aaactcattg aactcagacg cgccaaaatg
     7741 ttggcgctct ctttactgct tatcgccgct gctacctttg tcgttacgct gtttttgcca
     7801 cccaattttt gggtgagcgg cgtgaaggca attgctgaag cggcgatggt cggcgcgctg
     7861 gcggactggt ttgcggtggt ggcgctgttt cgccgcgtgc caattccgat catttctcgt
     7921 catacggcga ttatcccgcg taataaagac cggattggcg aaaatctcgg tcagttcgtg
     7981 caggaaaaat ttctcgatac ccagtcgctg gtggcattga ttcgacgtca cgaaccggcg
     8041 ttgctaattg gtaactggtt tagccagcca gaaaacgccc gccgcgttgg tcagcatctg
     8101 ttgcagatca tgagtggttt tcttgaactg accgatgatg cacgtattca gcgcctgctt
     8161 aagcgcgcgg tccatcgggc gattgataag gtcgatcttt ccggaaccag tgcgttgatg
     8221 ctggagagta tgaccaaaaa cgatcgtcat caggtgctgc tcgatacgct gatcgcacag
     8281 ttgattgctc ttctccagcg cgataaatcg cgcaagttta tcgcccaaca aattgttcgc
     8341 tggctggaga gtgagcatcc actgaaagcc aaaattctcc ccaccgaatg gctgggcgaa
     8401 catagcgcgg agttggtttc tgacgcggtg aattctttgc ttgatgatat cagccgcgat
     8461 cgtgcgcatc agatccgcca tgcatttgat cgcgccactt ttgccctgat cgacaagttg
     8521 aaaaacgatc cggaaatggc agcgcgagct gatgccgtaa aaagctatct gaaagaggat
     8581 gaagccttta accgctatct cagtgaattg tggggggatt tacgggaatg gctgaaagcg
     8641 gatatcaaca gtgaagattc tcgtgtgaaa gaacgtatcg cgcgggcggg tcaatggttt
     8701 ggcgaaacgt taattgccga tgatgccttg cgggcgtcgt taaatggtca tctggaacaa
     8761 gccgcgcacc gcgtcgcacc tgagttttcc gcattcctga cgcgccacat cagcgatacg
     8821 gtaaaaagct gggatgcccg ggatatgtcg cggcaaatag agttaaatat cggcaaagat
     8881 ctgcagttta tccgtgtcaa cggtacgctg gttggcggtt gtattgggct aattttatat
     8941 ttgctgtcac agctcccggc cttgttcccc ctcggcaatt tttagaaatc catgaaaaac
     9001 taagtggcta accgcgagag agatcaaata aaccacacta aaagaatggt aataatgtgg
     9061 cctcttttca ttatctcccg tggtacgggg aaggaaaatc atgtcacttg tcaccgatct
     9121 acccgccatt ttcgatcagt tctctgaagc tcgccagaaa ggctttctca ccgtcatgga
     9181 tctcaaggag cgcggcattc cgctggttgg cacttactgc acttttatgc cgcaagagat
     9241 cccgatggca gccggtgcgg ttgtggtttc gctctgttcc acctctgatg aaaccattga
     9301 agaagcggag aaagatctgc cgcgcaacct ctgcccgctg attaaaagca gctacggctt
     9361 cggtaaaacc gataaatgcc cctacttcta cttttctgat ctggtggtcg gtgaaaccac
     9421 ctgcgacggc aaaaagaaaa tgtatgaata catggcggag tttaagcctg ttcatgtgat
     9481 gcagctgcca aatagcgtta aagacgatgc ctcgcgtgcg ttatggaaag ccgagatgct
     9541 gcgcttacaa aaagcggtgg aagaacgttt tgggcacgaa attagcgaag atgctctgcg
     9601 cgatgccatt gctctgaaaa accgcgaacg tcgcgcactg gctaattttt atcatcttgg
     9661 gcagttaaat cctccggcgc ttagcggcag cgacattctg aaagtggttt acggcgcaac
     9721 cttccggttc gataaagagg cgttgatcaa tgaactggac gcgatgaccg cccgcgttcg
     9781 tcagcagtgg gaagaaggcc agcgactgga cccgcgtcca cgcattttaa tcaccggctg
     9841 cccgattggc ggcgcagcag aaaaagtggt gcgcgcgatt gaagagaatg gcggctgggt
     9901 tgtcggttat gaaaactgca ccggggcgaa agcgaccgag caatgcgtgg cagaaacggg
     9961 cgatgtctac gacgcgctgg cggataaata tctggcgatt ggctgctcct gtgtttcgcc
    10021 gaacgatcag cgcctgcaaa tgctcagcca gatggtggaa gaatatcagg tcgatggcgt
    10081 agttgatgtg attttgcagg cgtgccatac ctacgcggtg gaatcgctgg cgattaaacg
    10141 tcatgtgcgt cagcagcaca acattcctta tatcgctatt gaaacagact actccacctc
    10201 agatgtcggg cagctcagta cccgtgtcgc ggcctttatt gagatgctgt aaggagtggc
    10261 agtgacatat tcgattggca ttgattctgg ttctaccgcc accaaaggga tcttactggc
    10321 agacggcgtg attacgcgcc gtttcctcgt tccaacaccc tttcgtccgg caacagcaat
    10381 tactgaagcc tgggaaactc tgcgcgaagg gttagagaca acgccgtttc tgacgctcac
    10441 cggctatgga cggcaactgg tggattttgc cgataaacaa gtgacggaaa tctcctgtca
    10501 tgggctgggc gcacggtttc ttgcgccagc aacgcgcgcg gtaatcgaca tcggtggtca
    10561 ggacagcaaa gtgattcagc ttgatgatga cggtaacctg tgcgatttcc tgatgaatga
    10621 caaatgcgcg gcgggcaccg ggcgtttcct ggaggtgatc tcgcgcacgc ttggcaccag
    10681 cgtcgagcaa ctcgacagca ttaccgaaaa tgtcacgccg cacgccatca cgagtatgtg
    10741 cacagtgttt gctgaatcag aagcgatcag cctgcgctca gcgggcgtcg cgccagaagc
    10801 gattctcgcc ggagtgatta acgcgatggc gcggaggagt gccaatttca ttgctcgtct
    10861 ctcctgtgaa gcgccgattc tgtttactgg tggcgttagt cattgccaga cgtttgcccg
    10921 gatgctggaa tctcatctgg gaatgccggt aaatacccat cctgatgcgc aatttgctgg
    10981 cgcaattggc gcggcggtaa ttggtcaacg agtgaggaca cgccgatgaa agagttttta
    11041 tttttatttc actccacagt cggcgtcata caaacccgca aagcgttgca ggcagcgggc
    11101 atgacttttc gcgtcagcga tattccccgt gatttacgcg gcggctgcgg gttatgcatt
    11161 tggctgacct gtccccccgg cgaggaaatg caatgggtga tccctgggct gactgagtca
    11221 atttattgtc agcaggatgg tgtctggcgc tgcattgcac attaccgggt ttctccgcgt
    11281 taaggattca ttaaggtcgc tacgtatact tctcgatatc ttattgattt gccttaaagg
    11341 gattaccgtg acaaaaagta tttcgttaag taaacgcatc tccgttattg tgattctttt
    11401 tgccattgtg gcagtgtgta ctttcttcgt gcaatcttgc gcccgtaaaa gcaatcatgc
    11461 agcataattt cagaattacc acgccaccat tgacggcaaa gaaattgcag gcataacgaa
    11521 taatatctct tcgctgacct ggtcggcaca aagcaatact ctttttagca ctatcaacaa
    11581 acctgccgcc attgttgaaa tgaccaccaa tggtgatttt atccgcacaa ttcctctgga
    11641 ttttgttaag gatctcgaga cgatcgaata cattggcgat aatcagtttg tcattagcga
    11701 tgagcgcgat tacgccattt atgtgatttc actgacccca aactcggaag taaaaatcct
    11761 caaaaaaata aaaatcccgc tgcaagactc cccaaccaat tgcggttttg aagggttggc
    11821 ttattctcgc caggatcaca ctttctggtt ttttaaagag aaaaatccga ttgaggtcta
    11881 caaagttaac gggctgttaa gtagcaatga attacacatc agcaaagacg aggcactaca
    11941 acggcaattt actttagatg atgtgtccgg cgcagaattt aaccagcaaa aaaatacgct
    12001 tctggtgctg tctcatgaat ctcgcgcact ccaggaagta accctggttg gcgaggttat
    12061 cggtgaaata tcactaacca aaggtagtcg aggtttgtcg cataacatca aacaggccga
    12121 agggattgcg atggatgcct ctggtaatat ttatatcgtt agcgaaccaa atcgttttta
    12181 tcgctttacc cctaaatcat cgcattaaca ggctggcata tttcaccttt ttgttattaa
    12241 cttgttaaca acaaaaatga catacaaggt taaacatgcc ttcgtcctcg catcccgtag
    12301 aacgcttttc tttcagcacc gcgcttttcg ggatgctggt tctgacctta ggtatgggat
    12361 taggtcgctt cctttatacg cctatgttgc ccgtcatgat ggcggaaggg gcgttttcat
    12421 ttagccagct ctcgtggatt gccagcggca actatgcggg gtatctggct ggcagtctgc
    12481 tattttcgtt tggcgcattt caccagccgt cgcgcctgcg cccatttctg ttggtttctg
    12541 ccctggcgag cgggttgttg atcctcgcaa tggcatggtt gccgccattt attctggtgt
    12601 tattgattcg cttcctggcg ggtgtcgcca gcgccggtat gctgattttt ggttcgacgc
    12661 tgattatgca gcacacgcgc catccttttg tgctggcagc tttgttttct ggcgttggca
    12721 ttggcattgc actgggcaac gaatatgttc tggcaggcct gcattttgcc ttatcctcgc
    12781 aaacgttatg gcaaggcgcc ggggcgcttt ctggcatgat gctgattgca cttacgcttt
    12841 taatgccctc gaaaaaacac gccatcgcac caatgccatt ggcaaaaacg gagcaacaga
    12901 taatgagctg gtggttactg gctattctgt acggcctggc gggatttggt tatatcatcg
    12961 tcgcgactta cctgccgctc atggcgaaag atgcaggctc accattgtta accgcccatc
    13021 tctggacgtt agtcggctta tcaattgtgc caggctgctt tggctggcta tgggcagcaa
    13081 aacgttgggg ggctttgcct tgcctgacgg cgaatttgct ggtgcaggct atcagtgtgc
    13141 tgcttactct cgccagcgac tctcctctct tgcttatcat tagtagtatc ggttttggcg
    13201 gtacctttat gggcacgaca tctctggtga tgactatcgc ccgccaactg agcgttccgg
    13261 gaaatctcaa tcttttaggc tttgtgacgc tcatttacgg cattggacaa atcctcggcc
    13321 cggcgctgac cagtatgctc ggcaatggaa catcggcgct tgccggcgcc actctctgcg
    13381 gcgcggcggc gttgtttatt gctgcgctta tcagcacagt acaattattt aagctgcaag
    13441 tggtcacttc atgaggagtg tttcccttac cactcaagaa attcgaccgc aaccgttgat
    13501 gtcagccaga ccccgttttc cgtttgccag aaagggatgc ctcgtttcgc catttcctgt
    13561 gctttgacgg ttaagataac cggcgatccg tgtcttgctc ccactttgcg tgccgttgct
    13621 tcatcagcag agagatgtac gtagtgacgc tcacctgcaa tcagcccctg tttttttatc
    13681 tcgtcaagga aacggcttgc cgtaccgtgg taaagaaact gtggcggtgt tttttcggca
    13741 aaggaaatag cgacctgcga agtcaaatgc ccctgaaccg cgcggatgca cctaccatca
    13801 ctggaataac taaaacgctt tttatcactg gtggcgacca cggtatccag caatgtacgg
    13861 gttaatcgct tgcccgcttt ttgggcacaa agtatgagct tatcgatatc cgcccatcct
    13921 tcacggtcaa gtacaatacc aatcgcctcc ggtttgtgtc ggaggacaaa acttaaaaac
    13981 ttgctcgtat ccgccagctc tttttcgttg tactttgcca tctcgttttc gtccctggat
    14041 tttctgcttt tatttactga tgtcattgtc actgggttta tgaattttct gcaactcaat
    14101 cctgcgcaga gccttttcca cacagcgtaa tttttccgct atgcaaaaaa cgcatagcca
    14161 gaaacatact tgcattttcc ccatacccac atggccgata aggtttttca gctccaggaa
    14221 ttttcctggc tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt atgacacaac
    14281 aaggggatgc tgtagccggg gatctggcga cagaaaaagt gggcattaaa gggtatctgg
    14341 ctttttttct caccataatc tttttttccg gtgttttttc cgggacagac agctggtggc
    14401 gcgtttttga tttttccgtt ctgaacggat cgtttgggca actgcctggt gcgaacggag
    14461 ccaccacctc atttcgcggg gcgggtggcg cgggggctaa agatggcttt ctctttgcgc
    14521 tggagctggc accgtcagtc attctctctc tggggattat ttcaattacc gatggtcttg
    14581 gtggattacg cgccgcgcag cagttaatga cacctgtgct aaaaccgttg ttgggcattc
    14641 cagggatctg ctcgctggcg ctgatcgcta acctgcaaaa cactgatgcc gccgccggta
    14701 tgaccaaaga gctggcgcag gaaggggaaa ttaccgaacg cgataaagtg attttcgccg
    14761 catatcaaac cagcggtagc gccatcatca ccaactactt ctcttccggc gtcgcggtgt
    14821 tcgcctttct gggcacgtcc gtaatcgtac ctttagccgt catcctggtg tttaaatttg
    14881 tcggagccaa cattttgcgc gtctggctta acttcgaaga acgtcgcaac ccaacgcaag
    14941 gagcgcaagc atgacaactc aggtccgcaa aaacgtcatg gatatgttta tcgatggcgc
    15001 gcgccgtggc tttactatcg ccacgaccaa cctgctgcca aacgtggtga tggcgtttgt
    15061 catcattcag gcgctgaaaa ttaccggcct gctcgactgg gtggggcata tctgtgaacc
    15121 cgtaatggcg ctgtggggat tacccggtga agcggcaacc gtccttttag ccgcattaat
    15181 gagtatgggt ggcgcagtcg gtgttgccgc cagtctggca accgctggcg cattaaccgg
    15241 tcacgatgtc accgttttac tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt
    15301 cggtcgctgc ctggggacag ccgaagtgaa tgccaaatat tacccgcata tcattacggt
    15361 ctgcgtgatt aacgcattgc tgtcgatctg ggttatgcag cttattgttt aacaaggagt
    15421 taccatgatt gattataccg cagccggttt taccctgctg cagggagcgc atttgtatgc
    15481 gccggaagat cggggaattt gcgatgtcct cgtcgctaac ggcaaaatta tcgccgttgc
    15541 cagcaatatc ccttctgaca ttgtaccgga ctgcacggtt gtcgatctca gcgggcagat
    15601 cctctttcct ggttttattg atcaacacgt ccatttgatt ggtggtggcg gtgaagcagg
    15661 tcccacgacg cgcacgccag aagtggcgct aagtcgcctg acggaagcgg gcgtcacgtc
    15721 agtggttggt ctgctgggca ccgactctat ctctcgccac ccggaatccc tgctcgccaa
    15781 gacccgtgcg ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg cttatcatgt
    15841 cccttcccgc accattacag gttccgtgga aaaagacgtg gcgattatcg atcgtgtgat
    15901 tggcgtgaaa tgcgccatct ctgatcaccg ttctgccgca ccggacgttt atcacctggc
    15961 taatatggcg gcggaatccc gcgttggcgg tttgctcggc ggtaaacctg gcgtcaccgt
    16021 gttccacatg ggcgacagca aaaaggcgtt acagcctgtc tatgacctgc tggaaaactg
    16081 cgatgtgccg atcagcaagt tgctgccgac ccacgttaac cgcaacgttc cgttgtttga
    16141 gcaggcgctg gagttcgcgc gcaaaggcgg cactatcgat atcaccagca gcattgacga
    16201 accggtcgcc cctgccgaag gtattgcccg cgccgttcag gcgggtattc cgctggcacg
    16261 cgtcaccctc agctccgacg gcaacggtag ccagccgttc ttcgatgacg aagggaattt
    16321 aacccatatc ggtgttgccg gttttgaaac gttgctggaa accgtgcagg tgctggtcaa
    16381 agactatgat ttcagtatca gcgatgccct gcgcccgctc accagtagcg tagccggttt
    16441 ccttaacctg accgggaaag gcgaaattct gccaggcaat gatgctgact tgctggtcat
    16501 gacgccagaa ctgcgcattg agcaggtata cgctcgcggc aaactgatgg tcaaagacgg
    16561 caaagcctgc gtgaaaggaa cgtttgaaac ggcttaaacg gttgtcagcc gcccgaatgg
    16621 acgtagaatg cccgcaaggg cggctgacag agtaaaacgt aatggatgac tgtggtgcga
    16681 ttttgcataa tattgaaacc aaatggcttt atgattttct gaccctggaa aaatgccgca
    16741 atttttccca ggcggcagtc agtcgcaacg tctcgcaacc ggcattcagc cgccgcatcc
    16801 gtgcgctgga acaggcgatt ggtgttgaat tgtttaaccg ccaggtaacg ccgctgcaac
    16861 tctcagaaca agggaaaatc ttccattcgc agatccgcca tctgttgcaa cagttagaga
    16921 gcaatctggc agagctgcgt ggcggcagcg attacgcgca acgtaaaatc aagatagccg
    16981 ctgcacactc tctttccctc gggctgttac cgtccattat cagccagatg ccgccgctct
    17041 ttacctgggc aattgaagct attgatgtcg atgaagcggt cgataaactg cgtgaagggc
    17101 aaagtgactg tattttttcc tttcacgacg aagatttgct ggaagcgccg tttgaccaca
    17161 ttcgcttatt tgaatctcaa ttgttccctg tctgcgccag tgacgaacac ggagaagcac
    17221 tttttaacct cgcgcagcca cactttccgt tactgaatta cagccgcaac tcctacatgg
    17281 ggcgattgat taatcgcacc ctgacgcgcc acagtgagtt aagtttcagc accttttttg
    17341 tctcttcgat gagcgagctt ttaaagcagg ttgccctcga cggctgtggg attgcctggc
    17401 tgccggaata cgctatccaa caagaaattc gcagcgggca gctcgttgtg ctgaatcggg
    17461 acgaactggt gatcccgatc caggatctac gcataccgga tgaatactcg catgaatccc
    17521 gttgccgaac gcttctggcg tgaactgcgc gagctggaga ttgtgcttag ctgacattct
    17581 ccagcatcgc actacaccaa ccgccattat cccagaccag atcaaacgtt agcggttcac
    17641 cgtcgtggta cagcattatc tcaaggctga tcgccagagt gtggcagtac gcttctttaa
    17701 aattaacctg caacgccgca atccgtggct catgaaataa cagtgcagaa cgcagctgac
    17761 ggcaatacca gctctggaga tgtttgtcgc tctggttatg cacttgccac tcgggcatgc
    17821 ctgaaacaag taatgacggt aatgacgaat gaggagaatt aaaaagagtg aggagatcgc
    17881 tgcataccga tttacgcagc aatgacgttt tattgcctga aatttcaggt aaaacagcct
    17941 gacgtgattg gcgcatcatt ttgcgcactc ctgacgttta gcaacatatg cggcttgccc
    18001 tgaacaccgg gcaccactgt attgatgctc attttccata gccattccct tttgttcatt
    18061 acatccatgt cagtctgaga caggcctgaa tcctgtcagc aaagtataaa accttacttg
    18121 cctatgaata tctactgact tttcaggaaa aaagtcatga tttgataaaa atcatcaaac
    18181 tcaaaaaaaa accgctagta tattctgaat attccgaata gttacgtaga tgaaaatact
    18241 tgtcaccacc gctacttaat tccggtgtga aattatttat ttccgcataa tcagccatgt
    18301 attatttgaa acaattagtt
//
LOCUS       DAZGOB010000094        18073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGOB010000094 DAZGOB010000000
VERSION     DAZGOB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.094"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..298)
                     /locus_tag="ACHLIF_003455"
     CDS             complement(<1..298)
                     /locus_tag="ACHLIF_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607409.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFL"
     gene            complement(337..1566)
                     /locus_tag="ACHLIF_003456"
     CDS             complement(337..1566)
                     /locus_tag="ACHLIF_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO9607410.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(1865..2773)
                     /locus_tag="ACHLIF_003457"
     CDS             complement(1865..2773)
                     /locus_tag="ACHLIF_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFO9607411.1"
                     /translation="MARTLVNVSATIFALMMIVRALFTYIYPGKLPFSIAIIDWLIVI
                     AGSGAAISSIFCFIKKRYPDTAEFLPMFSTVCYVIILIGYAILRYTPAYQTSLSIMVT
                     GMLVGMGWWIQCITSAANTRRSHTLNMIINTRTSPEYQKQLRNSTKFYRGMRYVPQEL
                     SEWRCNPDKEEYKNMKVPDEYRDAINGLLYILNYFEFLAQGIKFKDLDDELLRECFSS
                     FLRGIERRGFHMILESQKQDPAAFEGIIYLSKKWNGTSFVETHRSNPNTVELGVPYPS
                     NDMVEKMVQGQPLIDSDAGPELQVAT"
     gene            complement(3256..4431)
                     /locus_tag="ACHLIF_003458"
     CDS             complement(3256..4431)
                     /locus_tag="ACHLIF_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO9607412.1"
                     /translation="MGKKEQGLLSLPRGVTIRKHKTASTLVITFTYKGVLCREPLSRL
                     EANTRGIKYAERLLGEIQNQIASGTFEYAKYFPNSKKLELFGVVKKTKNIKSYLDEYL
                     KICINRNLSPSTIGGYEKCLSALSELHKLHVTELTPAILKNWIASRKTKLKTIRNNLS
                     FLRSAIDEAVTDGLLTINPVTLVSASRYHVIDSSPSADDYEVDPFTPAETLAIYQSCR
                     YREWENLFRFAFNTGLRSSELCALRWADLDTIANTAHVQAASVVGVLKGTKTKAGTRK
                     VELNSEALAALQAQKQYTFMKSEFIFSDPKTGEPWTNADAIRKKAWVPTLKKAGVRYR
                     NPYQTRHTFATRHISQGVNLFWLAGQMGHKGPEMLFRNYGKYLAEYDGKTSINVVSS"
     gene            complement(4386..4595)
                     /locus_tag="ACHLIF_003459"
     CDS             complement(4386..4595)
                     /locus_tag="ACHLIF_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9607413.1"
                     /translation="MGLDCVPISTYCRNAGETVDAVNKRIQRGIWKEGVHVLKVDGVK
                     ERWVDLIEVSKWARKNKDYYLCQEE"
     gene            complement(4604..4750)
                     /locus_tag="ACHLIF_003460"
     CDS             complement(4604..4750)
                     /locus_tag="ACHLIF_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607414.1"
                     /translation="MHTVFELWVRKTFGNRYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LNVV"
     gene            complement(4754..4996)
                     /locus_tag="ACHLIF_003461"
     CDS             complement(4754..4996)
                     /locus_tag="ACHLIF_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9607415.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRCTDRSVFFRRMDGHSGRVCIG
                     EFNCLFEHIDHQQYCKILADTEQEKHLKKLRAMQRR"
     gene            complement(5081..5245)
                     /locus_tag="ACHLIF_003462"
     CDS             complement(5081..5245)
                     /locus_tag="ACHLIF_003462"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9607416.1"
                     /translation="MPNDAQWCVVNTEYGYYVQCWSEGQGWLGDDISIPECDVTHWMP
                     LPEPPQEVSQ"
     gene            complement(5333..5596)
                     /locus_tag="ACHLIF_003463"
                     /pseudo
     CDS             complement(5333..5596)
                     /locus_tag="ACHLIF_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            complement(5819..5944)
                     /locus_tag="ACHLIF_003464"
                     /pseudo
     CDS             complement(5819..5944)
                     /locus_tag="ACHLIF_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            complement(5946..6275)
                     /locus_tag="ACHLIF_003465"
     CDS             complement(5946..6275)
                     /locus_tag="ACHLIF_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9607417.1"
                     /translation="MSKIDYQALREAAEAIKVVATPQKLLAFRMKVTPQVVLVLLDEL
                     EAKNKRITELEAREVQLPTRYDLRYGHPINADKRHVMIPKENGSWLCLIDLEHALRVA
                     GIRIKGE"
     gene            complement(6272..6511)
                     /locus_tag="ACHLIF_003466"
     CDS             complement(6272..6511)
                     /locus_tag="ACHLIF_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607418.1"
                     /translation="MFKQNEKAIAQIADYIPRACRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTREAFFSRIAAQIRKGGNQ"
     gene            complement(6504..6707)
                     /locus_tag="ACHLIF_003467"
     CDS             complement(6504..6707)
                     /locus_tag="ACHLIF_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607419.1"
                     /translation="MTIVKTHTGTVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLLDTIKPIEVKHV"
     gene            complement(6704..7582)
                     /locus_tag="ACHLIF_003468"
     CDS             complement(6704..7582)
                     /locus_tag="ACHLIF_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFO9607420.1"
                     /translation="MCITHVVSFSGGRTSAYLVHLMEEQRKAGNNVCYIFMDTGCEHP
                     LTYRFIREVLKFWGIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGRGNYITWLGIRADEPRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWQKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYRSLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            complement(7573..8109)
                     /gene="yfdR"
                     /locus_tag="ACHLIF_003469"
     CDS             complement(7573..8109)
                     /gene="yfdR"
                     /locus_tag="ACHLIF_003469"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO9607421.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(8238..9062)
                     /gene="yfdQ"
                     /locus_tag="ACHLIF_003470"
     CDS             complement(8238..9062)
                     /gene="yfdQ"
                     /locus_tag="ACHLIF_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO9607422.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISNIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAMQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(9128..9490)
                     /gene="yfdP"
                     /locus_tag="ACHLIF_003471"
     CDS             complement(9128..9490)
                     /gene="yfdP"
                     /locus_tag="ACHLIF_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO9607423.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(10157..10831)
                     /gene="ymfK"
                     /locus_tag="ACHLIF_003472"
     CDS             complement(10157..10831)
                     /gene="ymfK"
                     /locus_tag="ACHLIF_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HFO9607424.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            10922..11122
                     /gene="ymfT"
                     /locus_tag="ACHLIF_003473"
     CDS             10922..11122
                     /gene="ymfT"
                     /locus_tag="ACHLIF_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO9607425.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            11166..11717
                     /gene="ymfL"
                     /locus_tag="ACHLIF_003474"
     CDS             11166..11717
                     /gene="ymfL"
                     /locus_tag="ACHLIF_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO9607426.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            11863..12042
                     /locus_tag="ACHLIF_003475"
     CDS             11863..12042
                     /locus_tag="ACHLIF_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9607427.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            11972..12865
                     /locus_tag="ACHLIF_003476"
     CDS             11972..12865
                     /locus_tag="ACHLIF_003476"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9607428.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGAGRG
                     NPEFTKAHNRALAVFLCHEQHYAQIMVGRAGPTSVGPGSLVTGISTPVRLTTNKVVES
                     LGGELLKITKEAAIMATIPTLTQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRTL
                     ECSASFTARNFSVSDYTDCTGRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFN
                     QMEKQLSKLAVPSDVAHNASVLYSYISSIHQVWLQQLYPMLEKAESPLAVSLYDRIND
                     AAFLARLIHSSLNSSEVRGRK"
     gene            12862..13086
                     /locus_tag="ACHLIF_003477"
     CDS             12862..13086
                     /locus_tag="ACHLIF_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607429.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            13083..13901
                     /locus_tag="ACHLIF_003478"
     CDS             13083..13901
                     /locus_tag="ACHLIF_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9607430.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARRDDPYFKASYDNVDYSQIPAGFRG"
     gene            13898..14392
                     /gene="yfdN"
                     /locus_tag="ACHLIF_003479"
     CDS             13898..14392
                     /gene="yfdN"
                     /locus_tag="ACHLIF_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9607431.1"
                     /translation="MIMSLLNDVQKFIEAHPGCASGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRQTEVLQESEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            14392..15045
                     /locus_tag="ACHLIF_003480"
     CDS             14392..15045
                     /locus_tag="ACHLIF_003480"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO9607432.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASSMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            15042..15368
                     /locus_tag="ACHLIF_003481"
     CDS             15042..15368
                     /locus_tag="ACHLIF_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO9607433.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     ERGLKV"
     gene            15365..15754
                     /locus_tag="ACHLIF_003482"
     CDS             15365..15754
                     /locus_tag="ACHLIF_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9607434.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISEAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            15774..16583
                     /locus_tag="ACHLIF_003483"
     CDS             15774..16583
                     /locus_tag="ACHLIF_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO9607435.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGGEQKKRPKYWLSNK
                     QTCELIEQLFTEGGIPPLEKNQPVSVINGGNNQGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            16591..17580
                     /locus_tag="ACHLIF_003484"
     CDS             16591..17580
                     /locus_tag="ACHLIF_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9607436.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKVLRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MATKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            17598..17939
                     /locus_tag="ACHLIF_003485"
     CDS             17598..17939
                     /locus_tag="ACHLIF_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9607437.1"
                     /translation="MRDMYEVLDRWGAWAAADNSGVDWQPVAAGFKGLLPHGKKTRQQ
                     CDDDEGIMIDSCVARLRKYKPEEYELVIAHFVIGISLRTIAKKQKCSDGTIRKELQTA
                     LGFLDGVLSML"
BASE COUNT         4509 a         4280 c         4414 g         4870 t
ORIGIN      
        1 tgagaaagtg aaaggcataa actaagtata cagctaaagt agccgttcca attgttggtc
       61 caatatcgcc ttgatcatga ggttcaaggt ttacattgaa cttgaagatt agccattcaa
      121 atccccaagt aaaaaaaaca actaatatca tggctgcgaa taatttcgcg atgttatctg
      181 ctgatggatt catcagaaca gattgtttct gaaaaaattc acaaagtgag agtccgaaaa
      241 aaataaaaaa aagtggtctg gaactaaatt tttcaaaaac tttgaatagg gtttccattt
      301 tctttacctt caggattata ttagctattc tgatacttag gctaccagag catttgatct
      361 ttgagcttga acgtaatcac tccaccattg catcaaactc tgtcgctcta tcagatattc
      421 tgcacgattg tatgctgcga taatttcatc ttttttcgag tgggcaagcg ctgcctcaag
      481 aacttcagct ctgaatttac cagactcctc tgccgctgtt cgtgcaatag aacgcatacc
      541 gtgagctaca agctcgcctc cgaaccccat tcggatgata gctgcgttgg ctgtttgttc
      601 atgcatatga ttaagaggcg cttttatgct ggggaaaacc cattctctat gcccacttat
      661 tgatttcatt aattcaagga tgcgcaaagc ttctttactc aaaggaactt tgtgaagctt
      721 tttcattttc atgaaatcag caggaatgtt ccaaatgctg ttggttgtat caatatcaga
      781 ccaccttgcg cgaacggctt cacccggacg aacccatgtc aacaattgcc attcaattag
      841 catacgtgtt tccaaccgga ttgacgcatt cgtcaaagat tccataaacc ttggcaattc
      901 gcttggggga agggcaggca tattttgctt ttttggttta ctgaatcttt gaccaaggtt
      961 gtcagccggg ttgaactcaa taagttcttc agtagctgcc caccggaaga tttcattcag
     1021 acgtgaaatg atacggcgta gagtttccaa tacgcctcgt tgctcaatag gatcaaggtg
     1081 ttgttttaag agcttaggtc ggatctcatt gatagggaca ttacccagac cgggaaagac
     1141 atttctctct aagctgcgcc agatgtctgc tgcatggtct tgtgagatac ctgatgtctt
     1201 taccttctca tctaaccatt tccgcgctac ggcttggaga gtgtgctcag tagcactctt
     1261 taatgccttc gccttatcgt tgttatggat ttggggatca acaccatttg ccagaaagga
     1321 gagatattca tcacgtaagg ctctggctct tgcaagggta aggtgaggat atgtcccaag
     1381 gctcattttg gttcttttct tgctcacggg tactgcatac ctgaaatacc aatttttctt
     1441 gcctcctttc gccaaaggag cgattcgtag aatcagacca tcaccgtcaa acaagttgat
     1501 ttctttatcg gctggcttgg tgcttttgat ttcagtgtca gtgagcttct tagcgatttt
     1561 tgccattttg ggaccctcgg tttttggacc cttcttagtg ggtcccattc agggtgccat
     1621 aactcgtagt tctcagcaat tctcactgga cgacaataga cgtaaaaaag cccgcagagc
     1681 ttgtgctgtg tgggcttagt agacttcatt gaacttcaaa caactaaaaa gtggagctgg
     1741 cgggagttga acccgtgccc gagcaatgct taacccattg aataacaatg cattctctgt
     1801 ttaaaaatgc ctcaagtgca ttttgagtgc attttctggt cctctcttgg tccatacaac
     1861 gagattaagt ggccacttga agttccggcc ctgcgtcgct atctattaac ggctgacctt
     1921 gaaccatttt ttcaaccata tcgtttgaag gatatggaac cccgagctca accgtattag
     1981 ggttggaacg gtgggtttca acaaacgatg ttccgttcca tttttttgaa aggtagatta
     2041 ttccttcaaa ggctgcaggg tcctgtttct gagactctaa gatcatgtgg aagccacgac
     2101 gttctatccc gcgtaagaag ctggagaagc actccctcag taattcgtca tccaaatcct
     2161 tgaacttgat accctgcgca agaaactcga aataattaag aatatataaa aggccattga
     2221 tggcatccct gtactcatcg gggaccttca tattcttgta ttcttctttg tctgggttgc
     2281 agcgccactc tgatagttct tgtggtacat aacgcatgcc ccgatagaat ttggtgctgt
     2341 tccgaagctg tttttggtac tccgggcttg ttcgggtgtt aatgatcatg ttcagcgtgt
     2401 gggacctgcg cgtgttagca gcggacgtta tgcattgtat ccaccatccc attccaacga
     2461 gcattcctgt aaccatgatc gaaagcgatg tttgataggc cggtgtgtac cttaaaattg
     2521 catatccaat aaggataatc acataacaaa cagtgctgaa cataggaaga aattctgcag
     2581 tgtctggata gcgttttttg atgaagcaga agatagaaga tattgctgca ccagaaccag
     2641 caatgacgat cagccaatca atgatggcga tgctaaatgg tagttttcca gggtagatat
     2701 aagtaaaaag tgctctgaca atcatcatta gagcaaaaat tgtagcgctg acgttgacta
     2761 atgttctcgc catcttattg attcctataa caaaagacct ccgcatggga ggccttattg
     2821 tgctaatgga ggtttgtttt aaaattttag gttgtagtta accttcaccg aaacctacgg
     2881 tcagtgtctt tttcatagtg ttcactccag ttgtggtgct tcgccgatac ctacgttaag
     2941 ctcttgaaaa gtaaaaaaac ataaaagttt tagaatttgt ggagcacagg ataacagttt
     3001 gtactgggac tcatcaagta ctatcagtgt ccttgcactg tcctagtgaa aatttattgc
     3061 aataaagtaa cttacgtgaa gtcaagaaat atatttgcac tccatttctt gctatgatgt
     3121 aagttattga ttttaattaa atttacaatt acctaaatga actatggaaa ctatcaataa
     3181 catagatgat agctcatttc acaaaaagca atggtagact cattcttaat tttcatcagc
     3241 cttaagccta tgtactcagc ttgacacaac atttatagac gttttaccat cgtattcagc
     3301 caggtattta ccgtaattgc ggaacagcat ttccggcccc ttatgcccca tctgcccggc
     3361 aagccagaaa aggtttacac cctggctaat gtgtctggtg gcgaatgtgt ggcgcgtctg
     3421 gtacgggtta cggtagcgca caccagcttt tttcagggtc ggcacccatg cttttttacg
     3481 gatagcgtcg gcgttcgtcc agggttctcc cgttttcgga tcgctgaata tgaactcact
     3541 tttcataaag gtgtattgct tctgcgcctg cagggctgcc agcgcctcac tgttcagctc
     3601 caccttacgg gtaccggctt ttgtcttggt gcctttaagt acccctacga cactggccgc
     3661 ctgaacgtgg gctgtgttcg cgatggtgtc gagatcagcc cagcgcagcg cgcacagttc
     3721 tgagctccgc agacctgtat tgaaagcaaa gcggaacagg ttttcccatt ccctgtacct
     3781 gcagctctgg taaatggcaa gggtctccgc tggcgtgaac gggtcaacct cgtaatcgtc
     3841 ggcgctcggg ctactgtcga tcacgtggta ccggctggcg ctgacgaggg ttaccgggtt
     3901 aatggtcagc aggccatccg taacagcttc atcaatggcg ctgcgcagaa atgacaggtt
     3961 attcctgatt gttttcagct tagtttttcg gctggctatc caatttttta gtatcgcagg
     4021 cgtcaattct gttacatgta gtttatgtag ttctgatagt gctgatagac atttttcgta
     4081 accgccaatg gttgacggcg acagattacg gtttatgcaa attttcaggt attcatcaag
     4141 ataggactta atatttttgg ttttctttac tacaccgaat aactccagtt ttttggaatt
     4201 ggggaaatat ttcgcatatt caaacgttcc gctggcgatc tgattttgta tctcccctag
     4261 caggcgctca gcatatttta taccacgtgt atttgcctca agccgagata ggggctccct
     4321 gcaaagaacc cctttatatg tgaaagtgat aactagtgtt gaagcagttt tatgcttacg
     4381 aatagttact cctcttggca gagataataa tccttgttct ttcttgccca ttttgaaacc
     4441 tctattaagt cgacccaacg ctctttaacc ccgtcgactt ttaatacatg taccccttct
     4501 ttccatattc ctctttgtat ccgtttgtta acggcatcaa ccgtttctcc cgcgttgcgg
     4561 cagtaggttg atataggtac gcaatccagc cccacagttc acctcacaca acattcagcc
     4621 cacgacagtg acaccacact tcaaacattc gtttcacaat ttcacgacag tagaaaccgt
     4681 caacatctcg cgtcaggtca tagcgattgc cgaatgtctt gcgaacccat aactcaaaaa
     4741 ccgtatgcat gtatcacctc cgttgcattg cgcgcaattt tttcaggtgc ttttcctgct
     4801 cagtgtccgc caggattttg caatactgct ggtggtcaat atgttcgaac aggcagttga
     4861 actcaccaat gcatacccgc ccggagtgtc cgtccatacg tcgaaagaac acagagcggt
     4921 ccgtacagcg agtaatcacc acagggtatc cggccctgtc cgtgtatatt tgcccgcgtt
     4981 gaataagtgc gaacatgaga ttatccccag cggcaaatcg aaaaaataat cagcgcaatc
     5041 gatattgcaa cacctacaat tgcaaatgct tcaggccaac tcattgactc acctcctgcg
     5101 gcggttctgg tagaggcatc cagtgggtta catcgcattc agggatgctg atatcatcac
     5161 caagccaccc ttgaccttca gaccagcatt gcacgtaata cccgtattct gtgttcacta
     5221 cgcaccactg tgcgtcgttc ggcattagct cactacagct tatccaacca tccggagtta
     5281 ccggaacttg cggaatggct gtctgctctc gaacgtcatt aggcgctata ggttctgctg
     5341 ccaactgact ggcatatttg ttaatggtaa cgataagctc ttgctcagcc tcatccagat
     5401 aatcaccgat acctcgcctg tcaccgtcaa aatcatcgaa atcggcacga atcttggcaa
     5461 ccttctggat tgcggacaac acctcactag gaattaccgg atagttggtt gacgtttccg
     5521 cgatttcccg aaaattattg gttgacgaat tcttattttc ccgaaagttt ctggcctgaa
     5581 gcatggcggc gcggcaggca ttccagccag tagcgtaata tttcgcatcc tgccagctcg
     5641 tttgtacatt tgagtacgct ttttcctctg tcatggcctc tggtattgat gccggtgcaa
     5701 ttcgtggcag gccgatatat agtggtacat ctcccggctc catcgaattg acgggccaaa
     5761 tatacgcatc actaccatgg ttaatgagcg caatccccga agtatcgatg atgccaaatg
     5821 gctccgctgt caatgcagtc aatgcaattt cataagcccg acgctcaata ttgtctcgca
     5881 cgtccaggct gcttatgcgc tctttgattt ctttaatcag tttcttatcg gtgaaagtgg
     5941 tcatctcact ctcctttgat gcgaatgcca gcgacgcgta gtgcgtgttc taagtcaatc
     6001 aggcaaagcc agctgccatt ttctttaggt atcatgacat gtcgcttatc agcatttatt
     6061 gggtgcccat atcgaaggtc gtagcgagtc ggtaattgaa cttccctcgc ttccagttct
     6121 gtaatgcgtt tgtttttagc ttctagttca tccagcagca ccagcacaac ctgcggtgtg
     6181 actttcatac gaaacgcaag caatttttgc ggcgttgcca cgacttttat tgcttctgcc
     6241 gcctcacgca gtgcctgata gtcaatcttg ctcactggtt gcctccttta cggatctgcg
     6301 ctgcgatgcg cgaaaaaaaa gcctcccgcg tatggctgtt aagagctggc gcgaacgccg
     6361 cgttaagaac ggcagcatca cagccgtcat cgatatagag cgcaattttt ttctccaggc
     6421 gcgctttggc ttcctgcaac tgcatacccc ggcacgcacg cgggatataa tcagcaattt
     6481 gagcgatagc tttttcgttc tgtttaaaca tgcttcacct cgataggctt gatggtgtct
     6541 aacagcagtc ggcggcgcgt attttctgca aaatggcggc gtccagtttc tttgtggtaa
     6601 aactcgtttt tgccaacgac ccacatccgc tctgtttggt gcaatttttt tacctgcggg
     6661 ccatctttgg tgatcacggt accggtatgg gtttttacga ttgtcatgcc acgacctctt
     6721 cgaatttcaa ctccaattgg tcaccccaga tttcacatga ttcggaacac gaaccagtat
     6781 caaaccgctt ggccagcacc atcgcctgat acaaacttct gtagtcgctg tcggcataca
     6841 ttctggcaat cccgtcaagc gtcaggtgac cacggtacat aacgtcttta cctgttctgc
     6901 gatgaccatc cctgacgtgt ttgcctgtaa ccagttcatt aaaaacccgc atcagaccag
     6961 gttcgtcttt acatgcaagc cccagctttt gcgttgactt tttgatgcaa aaaacacagt
     7021 tcccgaggtg ctccgggatt tgcaaatcaa aaggttgttt ttgccaccac cggataacat
     7081 ccgacttatc aaaatctgac agctcggcaa gataccggac gccaggtttc ggtttcagcc
     7141 tacggggttc gtccgcacga ataccgagcc atgtgatgta attccctcgc ccaaaatggt
     7201 catcgcaata tttcgtgaaa ggggtgagtt ttagcctgtc agtacagaat gcgccgccga
     7261 tgtatggcgt accgtacttt ttaaccatgt ccataaacgg tttaagcacc ggcattcgcg
     7321 tctgaatatc ctttggttcc cattccgtat aaccatttgg ctgcccaagc tctggattta
     7381 tatcgacctg caacacagtt agcggtatgc cccagaactt caaaacctcc cgaataaagc
     7441 ggtatgtcag cggatgttcg caaccggtat ccataaagat gtagcagacg ttattgccag
     7501 cctttctttg ttcttccatc aggtgaacaa gatatgcaga tgttctcccg ccagaaaaac
     7561 taactacatg agttatgcac atttgcgtaa ttccgataac tcgttgaagc gttccataaa
     7621 catcccgtag gcatggcccg gtgccagtgg aatcactttg aacatctctg ttgccgggat
     7681 accttccaat acaggccaga aagagccatc atcaagtccg agatcgcggc gttcggttgc
     7741 cagcatgatg agatcggcat atttcactgg cgtgctcata acaggaggta acccgtattt
     7801 ctcacggatt acggcgtcta ttttttcttc catccgttta tagtcaggaa gaagtcgttt
     7861 cagtggcgcg gggatgtcct ggcaatatgc ttctgttgca tcatgcatta acgcttcaaa
     7921 agcaaattcc tgcggcacca gctggctgca aagcaccgca tgctgggcga cactgtagaa
     7981 gtgtgaaaga tgtcctgcaa agcgacagat atttgaaagg gaaactgcga tatcgttaat
     8041 cacgatgtcg tctttattta tcttgtcata ataaaaatgc ttcccggaaa aagttttaat
     8101 aaatgacatt tcgttctcca ctttatatgc gctgcaccgc gctgaattct gctaaaagga
     8161 agctctcacc atccggtgat tattgagtta attacgtttc cataaatgcc cccgcagggg
     8221 catttgcagt aatgaaatca ggcggtgaaa gtaccaataa aggtttctac tttgctgtct
     8281 ttgaatttct caacaagcag atcacgaaat tcgttagcca tttcttcctg catcgcttcc
     8341 agctgaataa tgcgcagaac cagtacagga cgatcgccag tgataatgct gaggcgtaat
     8401 ttaaacggac gttctttcag gccttcaaac ggaacgcatt taaattcaaa tgccactggc
     8461 ataatgtctt tggtcttcgc ttcgacagac tccatcaggg agcgtttgcc gctgaagtca
     8521 ttgtcttcaa aatcagcggt ctggtttgct tcaattgtga ttttacggac tgccgcagcc
     8581 gctttggttg cctgaatggt gtcaccatta gcatcaaagc ccacaaggta gtcggcccag
     8641 tcttcaatcc attctgccag tgacttctgg gagttacgct cgccattaac agacaacaga
     8701 gcagaaaacg gtgctgtctt tttcagtttg agggtggcgg tgttatctgc gtgacctggt
     8761 tcatcaatag tacccaggtt aagcacactg acggctcgca tattatcggc atcgataaag
     8821 cagcgggtgc cttcatctgc aagatcttta gaataacggg taaagtcatc gatgctggca
     8881 gtggaaagcg caccacggaa acggaagcga tttaaattaa atttttccag atcatgaatg
     8941 cggaaattct caggcaatgc cacagcatcg gcaccaatat tactgataat ttcattaaca
     9001 ccctgagcag aaataagggc atggatttga ttaattgcgg ttgcgtctaa gttctgagac
     9061 ataataagtc ctcactatat aaagatattc agtgatgaga taaataatca gttaattaag
     9121 aacgatatta atgacctgct gcgcggagtt ttccgtcagg ttcaccggca agagtcagta
     9181 attgtccctg gtcttcctgc agaatagtca gtcgaccacc gcgattgaca tacatcggcg
     9241 tttcggtggt gtcttcttcg gaaattttcc cgcggttagt cgggcgaaca tatgagagtt
     9301 tgtgtttgat tttcacacgg ttctcatcaa acggttcgat ttccaggttg agcgagacct
     9361 tacctttggt tttcgtgttc atcacaccgg aagcgacttc actgagaact gcgccgattt
     9421 tggtttcaaa tacgccgccg tccagctccc cgataaatgc ctgcacatca gtactgcgtt
     9481 cgctagccat tttgctgctc ctcatcatat cgaccctgca aggtcggttg gtttctccac
     9541 aaaacagaga agaacacctg cggtggcagc cgcccggctg gattgggtta tgagcccgtc
     9601 gtccggtgat gctcttctct gttttgtaaa aagagcggta ccagccggaa gcaagtgtac
     9661 aaactggtac cgccaaagca gtggctgttg tggtggggtt gtcactcagg cgtatggtca
     9721 acctgacaat ccggtgtcct caacggggaa agagtaaccc cgccatactt accgccgcgc
     9781 catttcgcgg attaccacaa cgctgagagc acttagcaag ttacggcacc acactttgtc
     9841 gcggttccat aaatgccctc atcgttgcac cctggtctct tcccaggcgt caaaccggat
     9901 cgccgcgctg gttaggcgtc ttatcagcat catcattgac ttgcacattc cggctacctg
     9961 gtttttttgc ccgagcaagg agtggattgt cccctttaac gtccccagac cgctaacgac
    10021 gcatgtgcca tacgccgtgt tacaaccaaa ttttgtttaa tcttgcctgc ggcatgtttc
    10081 ttttagatac attatgtatc tcaagggtac attgtcaagt ataaaaaaac ctgccgaagc
    10141 aggttataaa tattgattag gcctttattt tgtatcttct tggttttcct gagaaaatta
    10201 ctgtaccaat tatagagcaa ttaccgttaa tcttaatgta aggctcaggc cagtttgggt
    10261 ttaatgcttt gagataacgc tgtgttccat cttctatcaa tcgcttgaag gtggtttcgc
    10321 ctgtatcgtg catcaatgca ataacgtcgt caccgtggca ggcagggact tcgggatcaa
    10381 caaaaatcat gtctcccggg cggtactcat caatcattga atcaccaatc acccgcaaga
    10441 tataagtcat ttcgccacag ggtacagggc agggataagt ttctgctgtg ctcaaatcaa
    10501 cctcagaata gccaacttct ttccatgctc cggcctgtac ccatgatatg acagggacta
    10561 acgttatttg tttgttagtg attgaaacat caggtttttt tgtgatgttc gtggtctggt
    10621 gttcttgatc aagccatccg acaggcaggt cgaaacattt ttcgatgtgc cgtgccatgc
    10681 tgtcaccgat atttttagta gcaccatctc ccataaacct gctggtctgg gttggctcgc
    10741 gatcaatcat ggtggcaaag gaagaattcc cgccaacacc atctctcagt tttctggcgt
    10801 tagaccgccg gatgtcatgg actgttttca taaagaaatt aaaacctttg taccgataag
    10861 gtacaagtat cttgaaggtt catattaatc atgtaatatg tatattggag gtacatattg
    10921 tatgaaagcg tattgggact ctttaaccaa agaacagcag ggcgagttgg ccggaaaagt
    10981 tggctcaaca ccaggctact tacggctggt tttcaatggt tataaaaaag ccagttttgt
    11041 gctggctaaa aaacttgagc aatgcacgtc aggtgcaatt acgaaatctg acttaagacc
    11101 ggatatctat ccgaaagatt aacagaacac cttcaatttt taaccacaga acgatgaggc
    11161 taaccgtggg taagtatcac tggaaagtag aaaaacagcc tgagtggtac gtgaaagctg
    11221 tcagaaaaac tatcgcagcg ttgccgggtg gttacgctga agcagctgac tggctggatg
    11281 taacagagaa cgcattattt aaccgccttc gtgccgatgg cgatcagatt ttcccgctgg
    11341 gatgggcaat ggttttacag cgcgcggctg gcactcacta cattgcggat gctgtcgcac
    11401 agtctgctgg tggggtgttc gtatcgcttc ctgaaattga ggaagtagag aacgccgata
    11461 taaaccagcg cctgctggaa gtcatcgaac agatcgggaa ttactcaaag cagattcgtt
    11521 cggcaatcga agatggggtc gtggagccac acgagcagac agcaattaat gatgagttgt
    11581 atctgtcaat ttcgaagctc caggagcatg cagcactggt ctacaaaatc ttttgcgctc
    11641 cagaaaagag tgacgcccgc gagtgtgcag ctccgggcgt cgtggcgttt tgtgtctgtg
    11701 gagaaactaa cgcatgaaca gtttaacggc aaataaccgt ttgtcgcaac agctggtggt
    11761 cagtgtcgct gaacacctgt tgttacggca tgaatgcaga ttaccaaatc acctggctgt
    11821 aagtaaccac agagaacttt acctgactgt ggggggcgag ttgtgcagga acttaaccgc
    11881 tggtttcgtg acggaagagg gctttatgtc catgttattc gttgggagcc agaaacacag
    11941 cgcgttatct atcttcgcaa agactacccg catgagtgct ttagtccttt gtggaaattc
    12001 aggcgtgatt ttgttgagtg tgaaggacca ccagcacatt gattctgcca ttccgggacg
    12061 ttacactgtt caggcacctt ataaagcggg tgccgggcgt ggaaacccgg aattcaccaa
    12121 agcgcacaac cgcgctcttg cggttttttt gtgtcatgag cagcattacg cccaaattat
    12181 ggtggggcgt gcagggccaa cttcggttgg gccgggttct ttggtgaccg gtatttccac
    12241 ccctgtacgt ctcaccacca ataaggtcgt ggaaagcctt ggtggtgagt tattaaaaat
    12301 caccaaagag gctgccatca tggctacgat cccaaccctc actcaacccg aaattgccat
    12361 cgttgatggt caggctgtta cttcatccct ggctgttgcc aacttcttct ccaaacgtca
    12421 tgacgatgtt ctgaaaaaga tccgcacgct tgaatgctcc gcatcattca ctgcccgcaa
    12481 tttttcggtg agtgattaca ccgattgcac aggccgcaaa ctaccttgct atcaaataac
    12541 ccgcgacggc tttgcgtttc ttgctatggg tttcacgggt aaacgtgctg cccagttcaa
    12601 agaggcatac atcaatgcct ttaaccagat ggagaaacag ctttcaaagc tcgctgtacc
    12661 gagtgacgtt gcacataacg ccagcgttct ctattcctac atttcatcaa ttcatcaggt
    12721 ctggttgcag cagctttatc ccatgctgga aaaagctgaa tcaccgctgg ctgtaagtct
    12781 gtatgaccga attaacgatg cggcatttct tgcccgtctt attcattcgt cgctgaactc
    12841 ttcagaggta agggggcgca aatgatccga aatattttca aacggtttac caatcaaact
    12901 ttccgttgtc ctcgcccggg tcagtggtac accacgcctg cagggcatgt tctacgtgtt
    12961 agcctggttg accgtgaatg tcagaaggtg atttgtgaac cgctgggtcg taattaccgc
    13021 gtcagtatgc cgctcatagc ctttcgctcc ggaaaaaaca tgaagcatct cggaggtgca
    13081 gcatgagtat ggagctgatg gttaaagcga tgaaaattcg agtgggtaat ccattgcgaa
    13141 aactggttct gatcaagctg gctgataatg ccagcgatca gggtgagtgc tggcccagct
    13201 accagcatat tgctgaccag tgcgagatta gcaaacgttc tgtgatgaat catattgcgg
    13261 ccctttgtga gtccgggctg gtaaaaaaag tcacccggaa aggtgaaaaa ggtaactcaa
    13321 gtaatatcta tctccttcat ctggatggtg caggagattc actagggggt agtgcaaata
    13381 attcactatc tggtgcagca aattcaccag gtagtgcagg agttgcacca gggggtagtg
    13441 caggagattc acccagaacc agtcactctt ttgaaccagt caaagaacca gtcaatgaac
    13501 caatagctgt tggtgcatct gctgatgagt ctgtgcgagt tcgttcaaac cgaccggaat
    13561 actctccgga gtttgagcag gcatggctgg cataccccaa acgtgctggt ggcaattcaa
    13621 aatcagcagc cttcaaagcc tggaaagccc gtttgaacga gggggtaaac cccgaaacca
    13681 tgctggaagg tgtgaaacgc tacgcgggct gggtatctgc gatgggcaat agcggcacac
    13741 aatttgtgaa acaggctgtc acgttctttg gtccggatcg tcatttcgaa gaatcctggg
    13801 aagttcctgc ggtatctgca gccagacgcg atgacccgta cttcaaagcc agttacgaca
    13861 acgtggacta cagccagatc ccggcaggat tcagggggtg atcatgagtc tgttaaatga
    13921 cgttcagaaa ttcattgaag cccatccggg gtgtgcttcc ggagacattg cggatgcttt
    13981 tgcaggttac tcacgacagc gcgttctgca gtcagcaagc aagttacgtc agagtgggcg
    14041 tgtggctcac cgttgtgaag gggatactcg cagacatttc ccgcgccaga ctgaggtttt
    14101 gcaggaatcg gaaccgcaac cagttcgtga aaccagacct gtgcgcaatt tctatgtcgg
    14161 cactaacgac ccccgggtga ttttgtgcct gacccgccag gctgaagaac tggagtccag
    14221 gggcttatac cgtcgtgctg caacggtgtg gatggcggca ttccgtgaaa gccactccca
    14281 gccagaacga aacaattttc tggcgcgtcg tgagcggtgc ttacggaaaa gcagcaagcg
    14341 cgctgcatcg ggtgaagagt ggtatctgtc agggaattac gtgggggctt aatgagtaat
    14401 aaatattgcc aggcgctggt ggaactgcgg aacaaaccag cccatgaact gaaggaagtg
    14461 ggcgatcagt ggcgcacgcc ggacaacatt ttctggggaa ttaacaccct gtttggcccg
    14521 tttgttctgg atctgtttac tgacggtgat aacgccaaat gtgccgcgta ttacacggcg
    14581 gaagataacg cgctggcgca tgactggtca gaacgtcttg cggagcttaa aggtgctgcc
    14641 tttggtaatc ccccatacag ccgcgccagt cagcatgagg ggcaatacat caccggcatg
    14701 cgttacatca tgaaacatgc cagttccatg cgtgataagg gcgggcgcta tgttttcctg
    14761 atcaaagctg ccaccagcga agtgtggtgg ccggaagatg cggaccatat tgcttttatt
    14821 cgcgggcgta ttggttttga actgcctgcc tggtttatcc cgaaggacga gaagcaggtg
    14881 ccgacaggag cttttttcgc tggtgctatt gctgtttttg acaagacctg gaagggaccg
    14941 gcaatcagct acatcgggcg cgatgaactt gaggcatgtg gtgaggcgtt tctggcgcag
    15001 gttcgccagc aggcggaaaa actggtcagg gagatggcgg catgacgacg ttaactcaat
    15061 gccagcagca ggtgctggat atgctgattt cttatcagaa agaacgtggc ttcccgccaa
    15121 ccaatcagga ggtggcaacc atgctgggat accgttcggt gaatgcagcg gtggagcatc
    15181 ttcgcgcact ggagaaaaaa ggcgtcatca cgataaagcg tggtgtggcc cgggggatca
    15241 cgcttcatac cgcggtgaag gacgacgaca gcgaggcggt cgggattatc cgctcactgc
    15301 ttgccggtga ggaaaacgca aggctgcgtg caactcactg gttacatgag agaggcctga
    15361 aagtatgaag ctgatcctgc ctttcccgcc cagcgtgaac acgtactggc gacaccccaa
    15421 caaaggggca tttgctggta agagcctgat aagcgaggcg gggcgaaaat ttcagagcgc
    15481 ggcgtgcgca gcaatagttg agcagttacg tcgtctgccg aaaccaacgt cggcacctgc
    15541 ttcagtggag atcgtgttgt ttcctccgga taaccggatc cgcgatctgg acaactataa
    15601 caaggcgctg tttgacgccc tgacccacgc gggtgtgtgg gaagacgaca gccaggtgaa
    15661 aagaatgctg gtggagtggg gaccggttat cccgaaaggg aaggtcgaga tcactatcag
    15721 taagtatgag aaaccggcgg gtgcagccgc ctgattaaga ggagaaacga agtatgaata
    15781 atctgatggt cattgatggt attgaagttc gtcgtgatgc ttatgggcgt tacagcctga
    15841 acgatctgca cagggcagcc gggggagaac aaaaaaaacg cccgaaatac tggctctcca
    15901 ataagcaaac ctgtgaattg attgaacaac ttttcaccga gggtggaatt ccgcctctgg
    15961 aaaagaatca accagttagc gttattaatg gcggaaataa ccaggggacg tatgtctgca
    16021 aagaactggt gtatgcctat gcaatgtgga tcagcccgtc attccatctg aaggtgatcc
    16081 gtactttcga tatggtaacc agcgcatcgg aaaaattatc cgggcaggct gctgacaaga
    16141 tgcaggctgg cgtgattctg ctggacttta tgcgccggga gttaaacctg tctaactctt
    16201 cagtgcttgg tgcctgtcag aaactccagg aggctgttgg cttaccgaat ctggcaccgc
    16261 gctatgccat tgatgctcct gctgacgcgc ctgatggctc aagccgtccc acgctgtcac
    16321 tgagtgcact gctgaagcag tatggtatcc gcctgacagc taatcaggca tatcaccaga
    16381 tggtgaagct ggggatcgtc gagcagcgcg aacgatacag ccgtaccgcg attaacaaca
    16441 tcaaaaaatt ctggtcgctg acagcgaaag gctgcatgtt cggcaagaac atcaccagtc
    16501 ccgcaaatcc gcgcgagacg cagccgcatt tcttcgaatc ccgattccct gagctgttaa
    16561 agctgctcga taccgttcat tgaggtgacc gtgagagcac tactgacccc tgaaattgcc
    16621 ccgcgtatgg ggatcgtatt gttcaggcca ggttcagagc tgatgcccct gtttatgcag
    16681 gggcgtgtcc tgctggagcc tgagccggaa cgttattcat ctttcgccag tggtgccgtt
    16741 ccggcggcat cacaaccgct ggcggatgat cctgccgttc gggccgtgtt ccgcaatgag
    16801 gcagtgatcc gtcgtgctgg tggcgtggaa tgtcttgaaa gctggttact tcgtgaaaaa
    16861 ggctgccagt ggcctcattc cgactggcac agcgagaaca tgaccacaat gcgacacgcg
    16921 ccgggcgcaa tccgtctgtg ctggcactgc gataaccagc tgcgcgatca gttcacggaa
    16981 cggctggaat caatggcaac ggataactgt gcccgctggg tgttgtctgt cgtgcgtcgg
    17041 gatctcggtt ttgatgacag tcacgttgtg acaatgccgg aactgtgctg gtggctgatt
    17101 cgtaatgatc tggcggatgc cttaccggaa agtgcagccc gtaaggtact gagattaccg
    17161 aagcctgttg tgccgtctgt cacccgggaa agtgaccttg tgccttcggt tcctgccacc
    17221 agcatcatcc aggataaagc gaaaaaggtg ctggcgctga aagtggatcc ggagtcgccg
    17281 gagtctttta tgttacgccc aaaacgtcgc cgctgggtta atgaaaagta cacgcgatgg
    17341 gttaagacgc agccgtgcgc atgttgtgga aaacctgctg atgatcccca ccacctgata
    17401 ggtcacggtc agggtggaat ggctacaaaa gcgcatgacc tctttgtgtt gcctttgtgc
    17461 agaaagcatc acgacgagct gcatgcggat accgtggcat ttgaagagaa gtatggctcc
    17521 cagctggagc tgatatttcg ttttatcgat cgtgcactgg caattggcgt gctggcctga
    17581 ttttgtggag aaagttgatg cgtgatatgt atgaagtatt ggaccgctgg ggtgcatggg
    17641 ctgcagcaga taacagtggt gtggactggc agcctgttgc tgcagggttt aaaggtttac
    17701 tacctcatgg aaagaaaaca cgccaacaat gcgatgatga tgaaggaatc atgattgaca
    17761 gctgtgttgc gcgattgcgg aaatataagc ccgaagagta tgagttggtt attgctcatt
    17821 ttgttatcgg catctcacta agaactattg caaagaagca gaagtgttct gatgggacaa
    17881 taagaaaaga gttgcaaaca gctcttggtt ttctggatgg tgtgctttca atgttgtaat
    17941 attaggggga attacccccc tttttttctc tgttgcttta ataaaatttt aatattttgt
    18001 ctgattatga tgaggcaatg taataaaaga aataccgtta gtattgcaag ccatacgcaa
    18061 aataagcatg cat
//
LOCUS       DAZGOB010000095        17941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGOB010000095 DAZGOB010000000
VERSION     DAZGOB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.095"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(274..615)
                     /gene="yebY"
                     /locus_tag="ACHLIF_003486"
     CDS             complement(274..615)
                     /gene="yebY"
                     /locus_tag="ACHLIF_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO9607438.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(628..1500)
                     /gene="copD"
                     /locus_tag="ACHLIF_003487"
     CDS             complement(628..1500)
                     /gene="copD"
                     /locus_tag="ACHLIF_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO9607439.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(1504..1878)
                     /gene="yobA"
                     /locus_tag="ACHLIF_003488"
     CDS             complement(1504..1878)
                     /gene="yobA"
                     /locus_tag="ACHLIF_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO9607440.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            2017..2247
                     /gene="holE"
                     /locus_tag="ACHLIF_003489"
     CDS             2017..2247
                     /gene="holE"
                     /locus_tag="ACHLIF_003489"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO9607441.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            2349..3005
                     /gene="yobB"
                     /locus_tag="ACHLIF_003490"
     CDS             2349..3005
                     /gene="yobB"
                     /locus_tag="ACHLIF_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO9607442.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            3029..3691
                     /gene="exoX"
                     /locus_tag="ACHLIF_003491"
     CDS             3029..3691
                     /gene="exoX"
                     /locus_tag="ACHLIF_003491"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO9607443.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(3688..5748)
                     /gene="ptrB"
                     /locus_tag="ACHLIF_003492"
     CDS             complement(3688..5748)
                     /gene="ptrB"
                     /locus_tag="ACHLIF_003492"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO9607444.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCIDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(5957..6616)
                     /gene="yebE"
                     /locus_tag="ACHLIF_003493"
     CDS             complement(5957..6616)
                     /gene="yebE"
                     /locus_tag="ACHLIF_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO9607445.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(6943..7299)
                     /gene="yebF"
                     /locus_tag="ACHLIF_003494"
     CDS             complement(6943..7299)
                     /gene="yebF"
                     /locus_tag="ACHLIF_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO9607446.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(7366..7656)
                     /gene="yebG"
                     /locus_tag="ACHLIF_003495"
     CDS             complement(7366..7656)
                     /gene="yebG"
                     /locus_tag="ACHLIF_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO9607447.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDSADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            7790..8968
                     /gene="purT"
                     /locus_tag="ACHLIF_003496"
     CDS             7790..8968
                     /gene="purT"
                     /locus_tag="ACHLIF_003496"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO9607448.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPAINGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(9024..9665)
                     /gene="kdgA"
                     /locus_tag="ACHLIF_003497"
                     /gene_synonym="eda"
     CDS             complement(9024..9665)
                     /gene="kdgA"
                     /locus_tag="ACHLIF_003497"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO9607449.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(9702..11513)
                     /gene="edd"
                     /locus_tag="ACHLIF_003498"
     CDS             complement(9702..11513)
                     /gene="edd"
                     /locus_tag="ACHLIF_003498"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO9607450.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(11748..13223)
                     /gene="zwf"
                     /locus_tag="ACHLIF_003499"
     CDS             complement(11748..13223)
                     /gene="zwf"
                     /locus_tag="ACHLIF_003499"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO9607451.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            13561..14430
                     /gene="hexR"
                     /locus_tag="ACHLIF_003500"
                     /gene_synonym="yebK"
     CDS             13561..14430
                     /gene="hexR"
                     /locus_tag="ACHLIF_003500"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO9607452.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14558..16000
                     /gene="pyk"
                     /locus_tag="ACHLIF_003501"
     CDS             14558..16000
                     /gene="pyk"
                     /locus_tag="ACHLIF_003501"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO9607453.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(16060..16902)
                     /locus_tag="ACHLIF_003502"
     CDS             complement(16060..16902)
                     /locus_tag="ACHLIF_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFO9607454.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASIDVLRKAVFEGANIIITHEPTFYSYADLEGDVLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDDMYHVYAIEP
                     DSAINITKRFAKNLKIYNLPGIQFYGDRARIVRTIGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGYSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
     gene            complement(16925..17764)
                     /locus_tag="ACHLIF_003503"
     CDS             complement(16925..17764)
                     /locus_tag="ACHLIF_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO9607455.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPLNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKL
                     FYC"
     gene            complement(17857..>17941)
                     /locus_tag="ACHLIF_003504"
     CDS             complement(17857..>17941)
                     /locus_tag="ACHLIF_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFO9607456.1"
                     /translation="ELKSKLTQSEFEQLMFTNAKKLFKIKI"
BASE COUNT         4562 a         4541 c         4281 g         4557 t
ORIGIN      
        1 ttaaatacaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg gaaatggctc
       61 ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc cttgcccttt
      121 aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa taaaaagcgt
      181 ggtggtcatc agctgaaatg ttaaaaaccg cccgttctgg tgaaagaact gaggcggttt
      241 ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag ctcgttctat
      301 aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct gaatcaccga
      361 aatgggttgg gctttagtct tcccactggc aacttccttt tgtgcgatat cgtttaaagg
      421 atactgcacg agggtacttg ggttaatgac atacaaagca ttacccggtc tgcaagtcag
      481 catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac gactgacggt
      541 aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa acgtcagaat
      601 actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa gcaggctgat
      661 gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccact caaaccaggg
      721 tgccgcacga tcttcatctt gcctcatgcg cggtacaaga acataacgat tagccagcgc
      781 aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc cccagtaagt
      841 cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc ttgccagtac
      901 gccgatcacc gcaaaatgcc cgcaccagga aaaacgcatc agcgcctgaa tggcctggtg
      961 tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc ccccaaacca
     1021 cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg ccgttacccc
     1081 ttcattcagt gtcgcatgcc cgattcccgc cagcagaata aattgcgcgg tagtgagcat
     1141 aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg ttacgagggc
     1201 gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag cctgccaaat
     1261 gtttggcgaa aatacatctg cccatcccgt tcccatcaac ccgccctgaa cggcgagcat
     1321 tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca gaaaacgctt
     1381 cgccagcaag cgacgaatcg ttaagggggc cagccaggca ccgtacattg cgaagccaaa
     1441 gaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa acgccagcat
     1501 gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca ccgaaacaac
     1561 atgccagtca acgatgtagg ttccgggttt cagcgaatcg gccagtggaa caatcagttg
     1621 cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt tttttggccc
     1681 cgtgattttt gcaccactga atcctgtttc aacaccttcc gagaagttta aagtgattgc
     1741 ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta aatgcgcatg
     1801 tgcccagacg gaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt agcgaaggga
     1861 gcgtgcagtt gaagtcatat tatctattcc tttttgtaat aactttttta cagagcataa
     1921 ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg gctcactctt
     1981 agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct ggctaaactg
     2041 gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt ggcatttaaa
     2101 gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc tgaacatttg
     2161 cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa tctgtcacgt
     2221 ttaccttacg agcccaaact taaataaaac ttatacagag ttacactttc ttacataacg
     2281 cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat tttgaaaagg
     2341 aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt aaaacctcac
     2401 tcaccgaaca ggtagctcat catctggagt ttgtgagggc cgccgccagg caacaatgtc
     2461 agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga cgtgcccttc
     2521 ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg acctggcgaa
     2581 tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt ggaattgcgg
     2641 tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt gcatgtctgg
     2701 gtcgtcgttc cagaaccatc acggtggtcg atgaacaacc gcaaggtatg gatatggacc
     2761 cgacctgttc actgtttact acagggcaat gtctgggaga acccgacctg ctggcgtcgg
     2821 cccgtcgctt acaatttttt tcacatcagt actcgattgc cgtgctgatg gcaaatgccc
     2881 gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc gatcgtggtt
     2941 cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc attccattac
     3001 gctaggcttt ttcggcctgg agcatgccat gttgcgcatt atcgatacag aaacctgcgg
     3061 tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa aaatcgtcaa
     3121 ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga tggcgattca
     3181 tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga tcccacacta
     3241 ttacggtagt gaatggtatg tcgcgcataa cgccagcttt gaccgccgcg tactgcctga
     3301 gatgcccggt gagtggattt gcactatgaa actggcccgt cgtttgtggc ctgggatcaa
     3361 gtacagcaat atggcgttat ataaaacacg caagctcaat gtacagacgc cgccgggcct
     3421 gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta tcgatattat
     3481 gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc cgtcgttgat
     3541 gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg ccgaacgcga
     3601 tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg agctgcgttt
     3661 aacactgaaa cattatctgg aaaatactta gtccgcaggc gtagcgggta atgttccctg
     3721 cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt taaagcgacc
     3781 agatttaccg ccatggcctg agtccatgtc gatacagagc aataaaagat ggtcatcggt
     3841 tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca cctgagaatc
     3901 gtgcaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt tgtcatacgg
     3961 gctgtagctt ttcatgtact cgtaatattg cggatcctgc ggattacccc actcttcaaa
     4021 ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat caacaaacgg
     4081 tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa cgcccatcaa
     4141 catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat agcccagttt
     4201 taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct tcagaaattt
     4261 tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga caatggcgta
     4321 gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat caatacttgc
     4381 gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa aatgtttgcg
     4441 atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc acaggtgttc
     4501 actgcggtaa ttcgccgcat caaaaccggg cacttctgtt tgttttaata cacgacgctc
     4561 accggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag aataaccata
     4621 acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat aggctggatc
     4681 atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca aactggttaa
     4741 cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc cttccagcat
     4801 gatgttttcg cgtggcggaa ttaactcttc ccactgttgc tcatcacgca tacgggtacg
     4861 gtataagcca aagtttttgc cgtggcggtt ggaacgtaga taaaaacgat gctggtagtg
     4921 atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct cggcatcggc
     4981 catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat gaatgactac
     5041 atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt tttcttcgta
     5101 gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct gataaggcag
     5161 cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg cccagacaaa
     5221 gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca gattacgaaa
     5281 acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa tggtgttatc
     5341 gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc gcttattggc
     5401 atcgagcaat gtttcccact catcccactc ttcactgaat gccgattgac gctggtagat
     5461 agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt tgatgtaggg
     5521 cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc gatcctgcaa
     5581 ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca ggtagtccag
     5641 gacttctggc tgagaacgcg tatcgtcccg cagccagtag taattatcga tgcgcgtatc
     5701 gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt tattgttctt
     5761 tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc gaaacagggg
     5821 agtcattgct tagatgatga caggtaatgg cgcggatatc gaatgttatg cagacagaga
     5881 aaatcagcct gttcaaatgg ctgtgcgatt ctggatagcc cgaaatagtc aacttcaggc
     5941 tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga tcgcgttcaa
     6001 tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc aggtaactgc
     6061 gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt tccagcgcct
     6121 cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt tcgattgcct
     6181 gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc tggtcgattg
     6241 ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc agcgcaagga
     6301 tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc ggttcgtcct
     6361 gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc gctaccgctc
     6421 cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt gctgatttat
     6481 ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt ttgcccaatc
     6541 cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat tgcagttgat
     6601 ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta gcatagcgga
     6661 tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg ggcggtaggc
     6721 cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt gataagggta
     6781 ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat tgcccgatgc
     6841 gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg acaaggcact
     6901 cgcgccgcat ccgccaacgg tcaatcgaat tatcagaagc cattaacgcc gctgatattc
     6961 cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac ttttaccacg
     7021 cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca aactgaccca
     7081 agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg ccacgcgatt
     7141 ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac cttcacactt
     7201 tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg cgcaggcaga
     7261 aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca tagcacaatg
     7321 attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg ccgcgtgtga
     7381 ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt tgccggtttt
     7441 cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg cttcacgttg
     7501 ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat ccgccgaatc
     7561 aagcatttta tcataagcat cggcttcctt tttgctggta aacgacattt tctcttcgcc
     7621 ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat aataactgtg
     7681 attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa agacacacgc
     7741 aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta tgacgttatt
     7801 aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg gtgaactggg
     7861 taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg tcgatcgcta
     7921 tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata tgcttgatgg
     7981 tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc cggagatcga
     8041 agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg ttgtcccctg
     8101 cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg cggcagaaca
     8161 gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt tccgcgaggc
     8221 ggttgccgcc attggctatc cctgcattgt aaaaccggtg atgagctctt ccggcaaggg
     8281 gcagacgttt attcgctctg cagagcaact tgctcaggca tgggaatacg ctcagcaagg
     8341 cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg acttcgaaat
     8401 taccctgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccag taggtcatcg
     8461 ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc cgcttgccct
     8521 tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt atggtctgtt
     8581 tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct cccctcgtcc
     8641 acatgatacc gggatggtga cgttaatttc tcaagatctc tcagagtttg ccctgcatgt
     8701 acgtgccttc ctcggacttc cggttggcgg gatccgccag tatggtcctg cagcttctgc
     8761 cgttatcctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc agaatgccgt
     8821 aggcgcaggt ttgcagattc gtttatttgg taagccggca attaatggca gccgtcgtct
     8881 gggggtggca ctggctactg cagagagtgt tgttgacgcc attaaacgcg cgaagcacgc
     8941 cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa aaatgcccga
     9001 tccgcgatcg ggcattttga cttttacagc ttagcgcctt ctacagcttc acgcgccagc
     9061 ttagtaatac ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag ccaggaacca
     9121 ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg agaaatacca
     9181 cctgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag ggctttcacg
     9241 ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc catacccagc
     9301 atcagttcgg aaacagtgct gatccccgga atcagaggaa tagtcccttc ggtagcagct
     9361 ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc ttcggtgact
     9421 tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc aggcacttct
     9481 ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac ttccagaacg
     9541 cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag tttttttacc
     9601 acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt tttccagttt
     9661 ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt gatacaggtt
     9721 gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc acgtcctgtc
     9781 cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag ttccgcttcg
     9841 tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc cccgtcgcgc
     9901 acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc tgacggcact
     9961 ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa acaccggtcc
    10021 aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt cgcttttggc
    10081 ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc aaaggccggc
    10141 ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg gttctcaacc
    10201 ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac ttttgtccca
    10261 ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag tgatttttcc
    10321 gccccttccc gccagtccag ttcaccatta ttcagccatg gttcaagggt ataacgagac
    10381 agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt gagcagttca
    10441 cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc cggaccgttc
    10501 gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc atcccagtta
    10561 atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt agtggaacca
    10621 ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat catcttaccg
    10681 atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc agcggcagcg
    10741 gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg caactgcatc
    10801 cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt acatgttccc
    10861 ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac tttaccttcg
    10921 gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc catcggtcca
    10981 gacggcacaa acaccgcagg caaatgacca aacgacaggg ctgccatcgt cagacccggg
    11041 acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg ggacagcccg
    11101 accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat tccatcctgc
    11161 ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc aaccgcattc
    11221 gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg gtgcgcggag
    11281 agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct tttcaaagag
    11341 gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca tgccaactgc
    11401 gaacgatgaa cggtcgaagt tttcgcttgt tctatccggg cgagataagc agagcgagtc
    11461 tcgcgcgaac gttcaatgat tcgatttgtt acgcgtaaca attgtggatt cataaaggct
    11521 cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag aggatttatg
    11581 actgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt gtaataaaaa
    11641 aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct acaatctgtg
    11701 caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta ctcaaactca
    11761 ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc ccaggttccg
    11821 gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat ggagtctacc
    11881 catttccagg cttcttccac ttcgtcgcga cgaacaaaca gtgcctgaat accacgcatg
    11941 gtttccagca gcaaacgttc ataggcatcc gccagatgcg tctgattaaa ggtttctgaa
    12001 tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc aggaacttta
    12061 ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag tttattctgc
    12121 ggcagatcct gccacgattc tttaaacaga ttcagttcag gtgttttgaa atagaccacg
    12181 acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg cacaccggcc
    12241 cagcgccagt tatcaatgtc gacgcggatc gctacgaagg tttctgtatt gctgctcttg
    12301 ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa gcccgcagta
    12361 tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg cagagacttc
    12421 agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagacgg cggagacatc
    12481 gcaatcatgc agagaatttg cagcaggtgg ttttggatca tatcgcgcat ctgaccggct
    12541 ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt aatctcaaca
    12601 tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa acgcagcgcc
    12661 aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac ctggcactcc
    12721 tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga cgtccccagc
    12781 ggtttctcca tgactacgcg tgccggttta gcattcagtt tagcctcgcc aagccctttg
    12841 caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat acgatttttt
    12901 tgatccagca tcgcgccgag acggctgaat gcagcagtgt cattgacatc gagattacaa
    12961 aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc tttcatgaaa
    13021 gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc agcacgccct
    13081 acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg atacagggaa
    13141 ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag gtcacaggcc
    13201 tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg gtacttaagc
    13261 cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc ttgcgaaaac
    13321 tgtaaacgct tatccacccg tgcgattacg gtaaaagcgc gcaaagtgcg gcaaaactga
    13381 taaaaaaatc atcgttttcc attgggtaaa aatctgacac tgatcatgtt atgaaaaaaa
    13441 ataacaactt ttttatctgc ttttgtcatt aacacagcac acaggcgtaa tatctgacaa
    13501 aactacattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtt tctttacatc
    13561 atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc agagcgcaaa
    13621 gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat tgctgctctg
    13681 gcgctggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag catggacacg
    13741 cgcggttttc ctgattttaa acttcatctg gcacaaagtc tggcgaatgg cactccctat
    13801 gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa aatatttgag
    13861 tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc catcaaccgc
    13921 gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg ctcttcagcc
    13981 gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt ggtgtactcc
    14041 gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga cgtggtggtg
    14101 ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct ggctcgcgaa
    14161 aacgacgcca tggtgattgc cctcacctct gcgggtaccc cgctcgcccg ggaagcaacg
    14221 ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat ggtttctcga
    14281 cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg acgcggtgca
    14341 aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg ttttgataag
    14401 cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta atcttatacg
    14461 acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga tttcatgttc
    14521 aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc ttcgcagaac
    14581 aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg aaaaagttat
    14641 cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg aagatcacaa
    14701 aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg tggctattct
    14761 gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca aagttttcct
    14821 caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag gcgacaaaga
    14881 aaaagtcggt atcgactaca aaggcctgcc tgctgacgtt gtgcctggcg acatcctgct
    14941 gctggacgat ggtcgcgttc agttaaaagt actggaagtt cagggcatga aagtgttcac
    15001 cgaagtgacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac ttggcggcgg
    15061 tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg cggcgttgat
    15121 tggcgtagat tacctggctg tctccttccc acgctgcggc gaagatctga actatgcccg
    15181 tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg aacgtgcgga
    15241 agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg tggtaatggt
    15301 tgcacgcggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca ttcagaaagc
    15361 gttgatccgt cgtgcgcgtc agcttaaccg cgcggtaatc acggcgaccc agatgatgga
    15421 gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag caaacgccgt
    15481 tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgctgggc agtatccgtc
    15541 agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc cgagcatcaa
    15601 cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta ttgccatgtc
    15661 agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca tgaccgaatc
    15721 gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt tcgccatgtc
    15781 gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc cggtgcactt
    15841 tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc tacgcgataa
    15901 aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga tgagtaccgt
    15961 gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc cccggccgct
    16021 gatgaccggg gtatttagca acacataaat taggactact cattcaatcc aatcataatc
    16081 acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat agcttgcaag
    16141 acgtcgcata cccgcttcct ctgcaacact atatccaata actgccatcg gtaagccact
    16201 atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg taatataata
    16261 atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc caatcccaat
    16321 agttctaact attctggctc tatctccata aaattggatc ccaggaagat tataaatttt
    16381 caaatttttg gcaaaacgtt tggtaatatt aatggcactg tcaggttcta tagcatatac
    16441 atggtacata tcatcagaag caacaatatt tgtaacatcc agttctaact gctgagccaa
    16501 agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac gaatgattac
    16561 caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat aactgagttc
    16621 accgcgcgta taatccatta tttttctggc ccatgaaaac tcaagaacgt caccctccag
    16681 atcagcataa gaataaaatg taggctcatg agtaataatg atattggcac cttcaaaaac
    16741 agcctttctt aaaacatcaa ttgaggcaat ccagcaggtc ccaattttat taataagtgt
    16801 cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacggcaa ccattgaatt
    16861 taaaaaacaa ttaagatctg cggctgtttt aatatgatgc attcaacatc cttcaccggt
    16921 ttcatcagca ataaaataat ttcaacattt ctggaaaacg ttttgcccat gcagactcag
    16981 agtgggtatc acctccccat agatgataat ctatagttac attgcgttgt ttacggagac
    17041 tcacgcataa tttttccgcg cctgaaatat agacttcagg aaagtcttcg cgcataccac
    17101 tggtttccag cgtaccgaca tcgagatata cagtaagatc tgcaaagata ttgttttcct
    17161 gaataaaatt cagcattgcg ctgtcattga accatagtgc cggcgaaaaa acgccaactt
    17221 ttgaaaaaac gtctagacga agaattgcgg caaacaaact gatgtacgca cccatagaac
    17281 tacctgctat cgctgtatta ttacgatctg ggagagttcg aaaattgctg tcaatatagt
    17341 tcttgagcgt attcacaata aaatcaacat acaacgcacc ttttcctcca ctataatcca
    17401 catcagggtc ccagccaggt aagggaaaag atgtgtttag cggccatgga gaatactcat
    17461 ccatacggct gaatttacca tgagacgatg cattatcaat ccccacaatg ataacaccgt
    17521 cgtattggga atttgggaaa aaagcatcta ccgcctcagg tatatcccat acatgtggac
    17581 caaaagatac agatttatca aataaatttt gcccatcgtg catatacaat acagggtaac
    17641 tgacatcaga ttcataatag gacgcaggta gtaatattct tatttcgcga ctacttgcca
    17701 attgtggcat cggaaaggat tgcaacacat gatatcgatt cccatagttc tcaatatcgt
    17761 tcataattta aattaacctc aataaacaaa agaaatatta catctaagca ctcccctacc
    17821 acattatata tgggagcact tagaaatgac taatatctat atttttatct taaaaagttt
    17881 tttcgcattg gtaaacataa gttgttcaaa ctcactttgc gttaatttac tttttaattc
    17941 a
//
LOCUS       DAZGOB010000096        17914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGOB010000096 DAZGOB010000000
VERSION     DAZGOB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.096"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..354
                     /locus_tag="ACHLIF_003505"
     CDS             <1..354
                     /locus_tag="ACHLIF_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO9607457.1"
                     /translation="NGTEIAITYVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEP
                     LSAKYKNIAGVEEKLTYTDTYAQENVTIDMEKVDFKALQGISGINVSAEDAKKGITMA
                     QMELVMKAAGFKEVK"
     gene            complement(394..864)
                     /gene="yehS"
                     /locus_tag="ACHLIF_003506"
     CDS             complement(394..864)
                     /gene="yehS"
                     /locus_tag="ACHLIF_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO9607458.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIFLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(911..1630)
                     /gene="btsR"
                     /locus_tag="ACHLIF_003507"
                     /gene_synonym="yehT"
     CDS             complement(911..1630)
                     /gene="btsR"
                     /locus_tag="ACHLIF_003507"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO9607459.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1627..3312)
                     /gene="btsS"
                     /locus_tag="ACHLIF_003508"
     CDS             complement(1627..3312)
                     /gene="btsS"
                     /locus_tag="ACHLIF_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO9607460.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3534..4265
                     /gene="mlrA"
                     /locus_tag="ACHLIF_003509"
     CDS             3534..4265
                     /gene="mlrA"
                     /locus_tag="ACHLIF_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO9607461.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            4325..4432
                     /gene="yohO"
                     /locus_tag="ACHLIF_003510"
     CDS             4325..4432
                     /gene="yohO"
                     /locus_tag="ACHLIF_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO9607462.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(4413..5144)
                     /gene="yehW"
                     /locus_tag="ACHLIF_003511"
     CDS             complement(4413..5144)
                     /gene="yehW"
                     /locus_tag="ACHLIF_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO9607463.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5149..6075)
                     /gene="yehX"
                     /locus_tag="ACHLIF_003512"
     CDS             complement(5149..6075)
                     /gene="yehX"
                     /locus_tag="ACHLIF_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO9607464.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(6068..7225)
                     /gene="yehY"
                     /locus_tag="ACHLIF_003513"
     CDS             complement(6068..7225)
                     /gene="yehY"
                     /locus_tag="ACHLIF_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO9607465.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7232..8149)
                     /gene="osmF"
                     /locus_tag="ACHLIF_003514"
     CDS             complement(7232..8149)
                     /gene="osmF"
                     /locus_tag="ACHLIF_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO9607466.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8360..10657)
                     /gene="bglX"
                     /locus_tag="ACHLIF_003515"
     CDS             complement(8360..10657)
                     /gene="bglX"
                     /locus_tag="ACHLIF_003515"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO9607467.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            10853..12568
                     /gene="dld"
                     /locus_tag="ACHLIF_003516"
     CDS             10853..12568
                     /gene="dld"
                     /locus_tag="ACHLIF_003516"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO9607468.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12606..13538)
                     /gene="pbpG"
                     /locus_tag="ACHLIF_003517"
     CDS             complement(12606..13538)
                     /gene="pbpG"
                     /locus_tag="ACHLIF_003517"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO9607469.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13712..14299)
                     /gene="yohC"
                     /locus_tag="ACHLIF_003518"
     CDS             complement(13712..14299)
                     /gene="yohC"
                     /locus_tag="ACHLIF_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO9607470.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14469..15047
                     /gene="yohD"
                     /locus_tag="ACHLIF_003519"
     CDS             14469..15047
                     /gene="yohD"
                     /locus_tag="ACHLIF_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9607471.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(15177..15938)
                     /gene="yohF"
                     /locus_tag="ACHLIF_003520"
     CDS             complement(15177..15938)
                     /gene="yohF"
                     /locus_tag="ACHLIF_003520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9607472.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(15991..17427)
                     /gene="mdtQ"
                     /locus_tag="ACHLIF_003521"
     CDS             complement(15991..17427)
                     /gene="mdtQ"
                     /locus_tag="ACHLIF_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO9607473.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17651..17734
                     /gene="yohP"
                     /locus_tag="ACHLIF_003522"
     CDS             17651..17734
                     /gene="yohP"
                     /locus_tag="ACHLIF_003522"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO9607474.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         4356 a         4909 c         4468 g         4181 t
ORIGIN      
        1 aacggcactg aaattgccat tacctatgtc tacaaaggtg acaaggtgct taagcaatct
       61 tctgaaacca aaattcaatt tgcttctatt ggtgcaacca ccaaagaaga cgctgccaag
      121 acacttgagc cgttaagcgc caaatacaaa aacatcgcgg gtgttgaaga aaaattaacc
      181 tatacagata cctacgcgca ggaaaacgtg actatcgata tggaaaaagt ggattttaaa
      241 gccctgcagg gtatttcagg aatcaacgtt tctgctgaag atgccaaaaa aggtatcact
      301 atggcgcaaa tggaactggt gatgaaagcc gctggtttta aagaagtgaa ataatcggtt
      361 ggcggtcatg ctctaaacat gaccgccaat tttttagcct tttttcacat gctggcgcgc
      421 tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg cattcgcgga agtttttatg
      481 atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa acgcggaact gctgttcggt
      541 gaggatagcc agaatgtcat cggttttcag cgaaaacgcg atgcgtaatt ttttcagcac
      601 gatgttgtta ttaatgcgac gttccggctc cagtgccgga gcagactcat ccttgccgcg
      661 tttttcataa atcaggccat tgaggaatga cgacaaaaaa atgtccggac aacgctgaaa
      721 accctcttcg tcttctttac gtagccagac ggcgatctgt tccgcggtgg cttcgacatt
      781 acccagcgcc agaatacgca ccaggtcatt attattggct ttcaaaatgt agcgcacgct
      841 gcgcagaata tcgttactta gcatgaggcc ttcaggtgtt gatgaggcaa aaagccattt
      901 tagcagtctt ttacaggcca atcgcctctt ttaagctttt cagataacgg cggctgaccg
      961 gcacggttaa gccattacgc aaaatcaact cggcctggcc gttatcttcc agacgaatct
     1021 cctgtaaatg cgcgaggtta accagatact gacgatggca gcgcagtagt ggtgtacgac
     1081 tttccagggt acgtaatgtc aattcggtaa agccctcttt cccttcgtgg ctggtaacgt
     1141 agacaccgct catccgactg ctgacaaatg ccacatcttt catttgcagc aaataaatcc
     1201 gactatgccc cgtacaaggg ataaatttca gcgcctgttg attttccggt aacagcgaaa
     1261 catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt tttctccagt cgcgcttcat
     1321 caattggctt cagcagataa tcaaaggcat gttcttcaaa ggctttaata gcgtattcgt
     1381 caaacgcagt gagaaaaaca atatacgggc gatgttccgg gtcaagcatc cccaccattt
     1441 ccagaccact gatgcgcggc atctggatat cgagaaacag cacatccggg cgcagtttat
     1501 gcaccgcgcc gatcccttcc acggcgtttg aacactctcc aacgatttca atatcgctct
     1561 gctcctgcaa aaatacgcgc aggttctccc gtgctaacgg ttcatcatcg acaattaaga
     1621 ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt attcgggtgt aactatcagg
     1681 ctcacaggcg acgcttattc catagtcatc gccaaaccgt tcacgtaaac gcttatccac
     1741 cagattcatc cccagcccac tggcattggt taccggttga tacaaaccgg cattgtcttc
     1801 gatctccagc atcaaatgtt gcccctcacg tcgggcgctg attgccactc gccctgtatc
     1861 cagcagttgt gatgtcccat gtttaatggc gttttccact atcggttgca gggtaaacgc
     1921 gggcaattgc tgctgggata attcttgcgg aatagcaatg ttgacctgca accgcgactg
     1981 gaagcgcgcc ttttcaattt gcagataagc gttcacatgt tcaatttcgt cggcgagagt
     2041 aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa gtggaaagat actgcaccag
     2101 ctggctggcc tgttcgctgt cgcggcggat caccgcttta atggtgttaa gcgcattaaa
     2161 caaaaaatga ggattcacct gggcgtgaag cagtttgatt tctgactggg tgagcatcgc
     2221 tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa agcagttgcg caatcccctc
     2281 gcccagcgtg cggtttattg aactgaataa acggtttttg gcttcataca atttgatggt
     2341 gcccatcacg cgctgatttt cgccacgcaa cggaattacc agcgtcgacc ccagtttgca
     2401 ttgcggatgc aaagagcaac gataaggtac ttcgttgcca tcagcgtaga ccacttcacc
     2461 ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt ttgccgggta aatggtggtc
     2521 gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg gtaatcgcga ctgcaccaat
     2581 atccagctcc tgatacagca cctgagccac tttcatgctg ttcacttcgt taaacccctg
     2641 tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca gtggcagaaa aagcagaagt
     2701 gtatttttca aacatcgcgc gtttatcgag caatatacgc ataaacagcg ccgcgccgac
     2761 ggtattggtg accatcattg gtgcagcaat attactcacc agacgcaccg catcttcata
     2821 aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt tcagcgacga acgtgacggc
     2881 accggcggta atggggttaa agactttatc agtgcgcccg cgacggatca ggatgctgtg
     2941 taccaggccg ccgagcaatc cttcaacgat ggttgagatc atgcaactta gcgcggtcat
     3001 gccccccatc gaatatcgat gtaagccgcc ggtcagacca accagcccac cgacgaccgg
     3061 accgccgagt aagccgccca ttaccgcgcc tatcgcacgg gtattggcaa tagaatcgtc
     3121 aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg gaaaagacga tgtagcagag
     3181 aaatttatgc ggcagacgaa ccgtgacctg cattaacggt atgaataatg gcgttttact
     3241 cattaaccac gcaatgacta aaaaaacgca catctgctga agcagcagca acaccagatt
     3301 aaaatcgtac atacccgcaa accacacttc cctttaaaac gcgtaacata cattgcctgc
     3361 gtttaacttt ctttgaactc ttgcagaaaa atgagaattc gtgagtacga tcactcaaaa
     3421 tcgcctggca aaaataaaat caccctatag atgcacaaaa aacgggcaaa actacctggt
     3481 tcgcaaaact gcgtctaaag ttaaaccggg acctcgcgag caagggtgag acgatggcgc
     3541 tttacacaat tggtgaagtg gcgttgcttt gtgatattaa ccctgtcacg ttacgcgcgt
     3601 ggcagaggcg ttacgggttg ctgaaaccgc aacggacaga cggcggtcat cgactgttca
     3661 acgatgccga tattgaccgt atccgcgaga tcaaacgctg gatcgacaac ggcgtgcagg
     3721 tcagcaaagt taaaatgctg ctcagtaatg aaaatgttga tgtgcagaac ggctggcgcg
     3781 atcagcaaga aacattactg acctacctgc aaagcggcaa tctgcatagc ctgcgaacgt
     3841 ggatcaaaga gcgcggtcag gattaccccg cccagacact caccacacat ctgtttattc
     3901 ctctgcgccg acgacttcaa tgccaacaac cgactctcca ggcgctgctg gcgatcctcg
     3961 acggcgtact gatcaactac atcgccattt gtctggcttc ggcacgtaaa aaacagggta
     4021 aagatgcgct ggtggttggc tggaatattc aggataccac ccgtctgtgg ctggagggct
     4081 ggattgccag tcaacaagga tggcgcattg atgtcctcgc ccactcgctc aatcaactac
     4141 gccctgaact attcgaaggt cgtacattgc tggtgtggtg cggtgaaaat cgaacctccg
     4201 cccaacagca gcaactcacc agttggcaag aacaaggcca tgatattttc ccactcggca
     4261 tttaatgatt cgttaacaaa tgcgctttac tgtacaatcc tttcgttaac ataaggagtg
     4321 cattatgcgc atagctaaaa ttggggtcat cgccctgttc ctgtttatgg cgttcggcgg
     4381 aattggtggc gtcatgctcg caggttatac ctttattttg cgtgctggct aagcgcctgc
     4441 accagccttt caaacaggcg gtctgcgatg atcgccgcca gtgccaccag taacgccccc
     4501 tggatcacat acgcggtatt aaatccgcta agcccgatga tgatgggcgt acccagcgtg
     4561 ctggccccta ccgttgaggc gatcgtcgcc gtaccaatgt tgataatcac cgaagttcgc
     4621 acgcccgcca gaatcaccgg agccgccagc ggtagctcga ccttacgcag tcgctgacca
     4681 cgactcattc ccataccttt cgcaacttct gtcacgctgg catcgatcgc tcccagcccg
     4741 gcaagtgtcg cctgcaggac gggcagcaca ccgtaaagga tcaaggcgat aatcgctggt
     4801 tgcagaccaa agccgatcac cggaacggca atcgccagca ctgcgacagg cggaaaagtc
     4861 tgtccaacgg cggcaatagt ttccaccagt gggcgaaatt ccgcgcccca cgggcgagtg
     4921 acagcaattc cggcaccagt gccaatgatc accgcaaaca aactcgaaat tcccaccagc
     4981 cagaaatgag ccagtgccag agctgcaaaa ctttcttgct gataaacggg tcgtggcagt
     5041 tgtgggaaca aggcagcaaa cagcggctgg ctgtaaggca gccagaaaat cagtgccaca
     5101 aacaaagcaa tgagccagaa cagcggatcg cgcaacatct tcatacgctt acgcctccac
     5161 cagcagatcc tgaaaatgca gcgtaccgca aggctggccc tgcgtgttca ccaccggcag
     5221 cacctcgcaa ccccgcgcga caaacaggga gagcgcatcg cgtagcgtca tctcttctgc
     5281 cagtgcctca ccttctgccc gttcttcgcg acgcacgtaa tccgccacac tacgtaacga
     5341 aagcagacgc acacccagtt cgctacgtcc aaaaaactgg cggacaaaat cattcgccgg
     5401 acgagtcagc atcgtcagcg gattgccctg ctgcaccact tcaccgtgat ccatcaatac
     5461 cagatgttca gccagccgta gcgcctcatc aatatcatga gtgaccagca caatagtgcg
     5521 ccccagcaaa cggtgaatgc gcgtcatctc ttgttgcaac gcgccgcgcg ttaccgggtc
     5581 cagtgcgcca aaaggttcat ccatcagtaa gacttgcgga tcggcagcca gtgcgcgcgc
     5641 cactcccaca cgttgctgct gaccaccgga aagctgatgc ggataacgct cacgcaaatt
     5701 tgactccagc cccagtagcg tcattaattc gtcgatacga tcgtcaatcc gtgcccgcga
     5761 ccatttttgt aattgcggca cggtagcgat gttttgcgcc acgctccagt gggggaatag
     5821 gccaatagat tgaatggcat agcccatccg gcggcgcaac tccagtactg gcagcgagcg
     5881 aatttcttct ccggcaaagc ggatctctcc gctgtcatgt tccaccaggc ggttaatcat
     5941 tttcaaggtg gtggatttgc cggagccaga tgtgccaatc agcaccgaaa aactcccttc
     6001 ctgaaaattg agattaagat cgttaacggc tttttgtgcg ccaaacagtt tgctgacatg
     6061 gctaaattca atcattacgt ttcaccttca gcagtgcgat aagcaaatcg aacagggcat
     6121 cgatcagcac cgccagaaca attaccggga tcacccccag caacactaaa tcaatggcgc
     6181 tgcttagcag cccctggaaa accagcgcac caaaaccgcc tgcgccgatt aacgccgcaa
     6241 tcaccgccat acctacagtt tgcaccatca ccacccgcag gctgcgcaga aataccggta
     6301 acgccagtgg taactgaaca tgcaggaatc gctgcgcccc gctcatgccc atcgctctgg
     6361 cgctctccag cacatcgcgc gggatctggt tcaaaccgac taccacgccg cgcaccagcg
     6421 gcagcaaggc atagagcacc agcgcaatca gtgcgggtgt cattccggtt cctgctatgc
     6481 cgagcttccc cagccacgga aaggccgtca ccagcgcggc aagcggcgca atcaacaggc
     6541 caaagagcgc caccgaaggc acggtctgaa tgacattgag cagagaaaaa attgccccct
     6601 gccgagcagt ggaaaagtag caccagatgc ccaacggcac accaatcact aacgcaggca
     6661 gcaccgcacc aaacagcaac gtcagatgtt gtgccagcgc gtcgtcaaac acatcctgac
     6721 ggttggcgta ttctttcatt agtgagagat cgttaagcgt gccggagtac agcaaccaca
     6781 gcggaataat ggcaatctgc atatgcaaca accagcgcca cagcggatgc gtggagattc
     6841 ggcggatggc atcgctacag gccagcaatg ccagcgccgc agccagccag aaaccactgc
     6901 cgaggctggt acgcgccagc gcactgccat tttgcgccag ttgtgtcgcc gcctttcccg
     6961 cgccccacac cagcaatacg aagacgaatt gcgccagaat gagtgcacaa atgctgcctt
     7021 ttttaccggg aataaaacag gccgtcagcc aggcgcaacc aacgcccacc agcatccaga
     7081 tcgtttgcgg ccacagttgc cagagatgac gcccttcacc cgagactaaa cgattaggcg
     7141 cgtagctgat aaacggcagc gctgctgcga ttgccgtcag caacagcagc agcgccagaa
     7201 caggattaat acgtagataa gtcacgggaa attacttcgc ccaccctttt tgtttcaggt
     7261 agtcggcagc cacttttttg gcatccagtc cttccactgc aatgctggca ttcagttgct
     7321 gcaatgtttt tgcatcgagg ctggcgaaga ctggctgtag ccactgtgcc atttgcggat
     7381 actccttcaa caccgattca cgcaccactg gtgcaggcgc atagataggt tgcacgcctt
     7441 gcggatcgct taaggtttgc agccccagcg ccgctaccga gccgtcagtg ccgtaagcca
     7501 ttgcagcatt aacgccagag gtttgctggg cagcggcttt gatcgtcacc gccgtgtcgc
     7561 caccagccag tgacagcaac tgatcctgac cgagcttaaa gccataggct ttttcaaacg
     7621 cgggtaacgc atcggcgcgt tcgataaact ctgccgaggc tgccagtttg aaggtgccgc
     7681 cctcttgcag ataacgactc aggtcggcaa gcgaagtgag tttatttttc tctgccacat
     7741 cctgacgcac ggcgatagtc caggtgttat ttgcaggcgc gggcgtcagc cagattaact
     7801 tgttgtgctc cgaatcgagc tttttgactt tctcgtaacc ttgctgcgcg tttttccacg
     7861 ctgcatcgtt ttcatcttta aagaagaaag cgccattgcc ggtatattcc ggatagatat
     7921 ccagttcacc ggaagtaatc gccccgcgca ccacaggagt cgttccgagt tgcactttat
     7981 tcaccgtcgg tacgccgtgg ctttccagca cctgcaaaat gatattgccg agtagcgcac
     8041 cttcggtatc gatttttgaa ccgactttaa cgggggaagc cgcctgtagc ggcaggctca
     8101 cggctgccaa cataaccagt gaacctgccc agagctttaa gagtggcatg atgctttcct
     8161 cattctttta ctgttgtttt cagcgaatta agagaaaagc atagttgata atggcggggt
     8221 tagcttgagc ggaaggattc agttgcagaa tcagataaat acttaagagg catattcggt
     8281 ccggcgtttc ctgccggatg cggcgcgagc gccttatccg gcctacaaag ggcgcaaacg
     8341 tcgcgccctt actaaagcat tacagcaact caaactcgcc tttcttaacg cgtgcggaat
     8401 cagtgccgat aaatacattg aacttgccag gctcggcgtc atatttcatc tgttgattcc
     8461 agaacttcag cgcctcaata tcgatcggga agctgacagt ctgagtttcg cccggtttca
     8521 gggtgatttt ctcaaagcct ttcagctgtt tcacagggcg actcatggaa gccgtcacat
     8581 cctgcaagta catctgcact accgtggccc cctcgcgctt accggtgttc gtcacctgca
     8641 cgctggcagt cactttgccg tcacgcttca tggtcggcgc agaaagtttc acatcagaga
     8701 cggtgaaagt ggtgtagctc agcccatagc cgaacggata caacgccccg ttagcttcat
     8761 caaaataacg cgaagtgtat ttgttcggct tgtcggcatt atacgggcga ccggtattca
     8821 gatggctgta gtacaccggg atctgcccga cagaacgcgg gaaggacatt ggcagcttgc
     8881 cggacgggtt gtaatcgcca aacaatacat cggcaattgc attaccgcct tcagtccccg
     8941 caaaccaggt ttccagaatc gcatcagcct gctgatcttc tttcaccagc gccagcggac
     9001 gcccgttcat cagcaccagc accagcggtt taccggtggc tttcagcgcc gcaatcaagt
     9061 cacgttggct ttgcggaata gtgatatcgg tccggctgga ggcttcgtgc gccatcccct
     9121 gtgcttcacc gactacagcc accaccacat cagattgttt cgccgtctgc accgcttcat
     9181 caatcatctc ttgcggcgaa cgcggatcga ctttgaccgc ttcttcatac tgattcagga
     9241 aatcgataat gcctttgtca ctggtaacgt tcgccccttt ggcatacagc actttaccgt
     9301 tttcaccgac ggaattttta atcccggtca gtacggtcac ggattgatcg gcaacaccgg
     9361 ctgcggacca gctgcccatc acgtcacgtt tactgtccgc cagtggccca accaccgcaa
     9421 tggtggccga ttttttcagc ggtaacgttt cgagacggtt tttcagcaac accaggcttt
     9481 cgcgcgccac ttcacgcgct tctttacggt gcaggcggct ttcggcattg gtatccaccg
     9541 ggtcagactc tttcggcccc aaatggctgt atgggtcgtt aaacaatccc atatcatatt
     9601 taacgttcag tacatggcgg gcagcatcgt ccagctcttc catcgtcact ttgccggatt
     9661 tgatcaaccc aggcagatac ttcgagtaat actcgtcgct catactcatg ttgattccgg
     9721 atttcagcgc cacgcgcacc gcatcttccg ggtctgccgc cgtgccatgt ttaatcagct
     9781 ctttgattgc accgtgatcg gaaacggtga tgcctttaaa gccccactgg tcgcgcagaa
     9841 catctttcag cagccaggaa tcggaggtgg ctggcgtgcc gttcagcgag ttcagcgcca
     9901 ccatcaccgc gccgctgcct gcgtccagcc ccgctttgta cggcggcata taatcattaa
     9961 acaggcgctg cggactcata tcgacggtgt tgtactcttt accgccttct accgcgccgt
    10021 atgcggcaaa gtgtttgacg ctggtcatca ccgagtagcg atctgccggg cttttaccct
    10081 gcatcgcttc caccatggtt ttacccattg ttgaggtgag atacgtatct tcgccaaaac
    10141 cttcggaagc acgtccccag cgcggatcgc gcgagacatc gaccatcggt gcccaggtca
    10201 tattcaggcc atcatctgcc gcttcataag cagagacacg tccgaccgtt ttcactgcat
    10261 cgaggttaaa agacgaggcc agaccgaggc taatcgggaa caccgtgcgc tgaccgtgca
    10321 gcacgtcgta agcaaagaaa agaggaattt tcaggcggct taattccatc acctgatcct
    10381 gcatggcgcg gatatcctga cgggttacgg tgttgaaaat cgccccaacc tgaccgtctt
    10441 tgatcatctc gcggatcgcc tctttcgggt tatccgggcc gacgctgatt aagcgcagct
    10501 gaccaatttt ctcatcaact gtcattttct taagcagttc ggtgacgaac gcatcccgcg
    10561 cttcgggcgt taatggatgg ttgccgaata aatcatccgc cagtgctggc tgcagggcca
    10621 gactcaccgc gattcctact gaacatagcc atttcatatg gatttttctt cctctgttgc
    10681 ccgacgaggc agcgaaaatg gtgcaaaaac cgtagtttgc cataagcatg atggagagag
    10741 aaaaagaatg ctcagtttat tgtctgaatt ttcaaaatat tcactcgctg aattgtcata
    10801 caaggcgcta ttctagtttg tgatattttt tcgccaccac aaggagtgga aaatgtcttc
    10861 catgacaaca actgataata aagccttttt gaatgaactt gcccgtctgg tcggtcattc
    10921 acacctgctc accgatcccg caaaaacggc ccgctatcgc aagggcttcc gttctggtca
    10981 gggcgacgcg ctggctgtcg ttttccctgg ctcactactg gaattgtggc gggtgctgaa
    11041 agcctgtgtc accgccgaca aaattattct gatgcaggct gccaatacag gcctgaccga
    11101 aggatcgacg ccaaacggta acgactatga tcgcgatatc gtgatcatca gcaccctgcg
    11161 tctcgacaag ctgcacgttc ttggcaaggg cgaacaggtg ctggcctatc cgggcaccac
    11221 gctctattcg ctggaaaaag ccctcaaacc gctgggacgc gaaccgcact cggtgattgg
    11281 atcgtcgtgt ataggcgcat cggttatcgg cggtatttgt aacaactccg gcggctcgct
    11341 ggtgcaacgt ggcccggcgt ataccgaaat gtcgttattc gcgcgtataa atgaagacgg
    11401 caaactgacg ctggtgaacc atctggggat tgatctgggc gaaacgccgg agcagatcct
    11461 tagcaagctg gatgatgatc gcatcaaaga tgacgatgtg cgtcacgatg gtcgtcacgc
    11521 ccacgattat gactatgtcc accgcgttcg tgatattgaa gccgacacgc ccgcacgtta
    11581 taacgccgat ccggatcggt tatttgaatc ttctggttgc gccgggaagc tggccgtctt
    11641 tgcggtacgt ctagatacct tcgaagcgga aaaaaatcag caggtgtttt atatcggcac
    11701 caaccagccg gaagtgctga ccgaaatccg ccgtcatatt ctggctaact tcgaaaatct
    11761 gccggttgcc ggggaatata tgcaccggga tatctacgat attgcggaaa aatacggcaa
    11821 agacaccttc ctgatgattg ataagttagg caccgacaag atgccgttct tctttaatct
    11881 caagggacgc accgatgcga tgctggagaa agtgaaattc ttccgtccgc attttactga
    11941 ccgtgcgatg caaaaattcg gtcacctgtt ccccagccat ttaccgccgc gcatgaaaaa
    12001 ctggcgcgat aaatacgagc atcatctgct gttaaaaatg gcgggcgatg gcgtgggcga
    12061 agccaaatcg tggttggtgg attatttcaa acaggccgaa ggcgatttct ttgtctgtac
    12121 gccggaggaa ggcagcaaag cgtttttaca ccgtttcgcc gctgcgggcg cagcaattcg
    12181 ttatcaggcg gtgcattccg atgaagtcga agacattctg gcgttggata tcgctctgcg
    12241 gcgtaacgac accgagtggt atgagcattt accgccggag atcgacagcc agctggtgca
    12301 caagctctat tacggccatt ttatgtgcta tgtcttccat caggattaca tagtgaaaaa
    12361 aggcgtggat gtgcatgcgt taaaagaaca gatgctggaa ctgctacagc agcgcggcgc
    12421 gcaataccct gccgagcata acgtcggtca tttgtataaa gcaccggaga cgttgcagaa
    12481 gttctatcgc gagaacgatc cgaccaacag catgaatccg gggatcggta aaaccagtaa
    12541 acggaaaaac tggcaggaag tggagtaaaa attacggatg gcagagtatc gccatccgaa
    12601 ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca tttgggcggc tttttgtttt
    12661 ttatagctca acgctgctgc cggcacaggc atcactttac cggtttcaat ccaggtacgc
    12721 aggcggctgg catcggcaaa atgggtatat ttgccaaacg cgtccatcac taccagcgcc
    12781 accggtttat tattgataac cgtacgcatc accagacaat ggcccgccgc attggtaaag
    12841 ccggttttgg ttaactgaat attccagtta tcgcgataca ccagatgatt agtattgcgg
    12901 aacggcagcg tatacgtcgg gttagagaag gttgccatat cttcccgggt agtacttaac
    12961 tgcccgatca acggatactg tttgctggca atgagcagtt tggttaagtc acgggcagtt
    13021 gaaacgttat gcaccgacaa tccggtaggt tcaacaaagc gcgtgttgtt cattccgagc
    13081 gatttcgctt tcgcattcat tgccttaata aaggctttgt aaccaccggg ataatggtgc
    13141 gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg ccagcaacag catatcttta
    13201 cggctgattt cgctattcag tcgtacgcgc gaatagaccc ctttcatctc cggcgtctgg
    13261 ctgatatcca cttttagttt ttcatccagc ggcagtcgtg catccagcac aaccatcgcg
    13321 gtcattaatt tgctgataga cgcaatcgga cgcaccagat ccgggtggtt cgaatagatc
    13381 actttgttgg tattcagatc aacaatcatc gcgctaccgg aggcaatttc cggttgtgaa
    13441 gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg ccaaaggcac agccagcatc
    13501 agggccaggc taaataaaga aactcgaaat ttcggcatga tgagcattca gatagtggtt
    13561 cacgcgcacg ggttgcgcac cgccggagta aggatttact gaggctagcg acgccatcat
    13621 aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagg aaatgctgca ttgctgacat
    13681 ttacgccagc aatgcaatgt caaagaactt tctagaacag gcgatacccg tagccccata
    13741 gtataacggt tagggcgagc agcacttcca gtaccagcac gccaatcgcc agcgtcgaac
    13801 tggagaagct aaggccttcc tctttgttga tattcaggaa gctcggaata ccaaggtaaa
    13861 gcaggtagcc ggtgtaaaac agcgccaccg tgccgaccag cgcacacaac cagaccagcg
    13921 gataaagcgc caccagaccg cttaaaaaca gcggagttgc aacgtagccc gcgaagacca
    13981 tgcagtgcgc aagtgacgga cgctgcgggt aattgcgcgc catccaccag atgacccgcc
    14041 ccatcaccgc aaccccagcg agcataacgc cataaaacag gacagccagc gccagtccgg
    14101 taaaccagga caacttcagg atagtgccat cgccaaaatt ccagccaatc tgtgtagtgc
    14161 caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa aacgtggtgg gtgtaatggt
    14221 gagaaatcgt ttcgttttcg cgattaatca cctgcatttc acgatcggga tgggaaaaca
    14281 gtccccagac atggctcata ccgccccctt gttgtgagtt catgaacctg acagttcaag
    14341 tataagtcag cttgtgatta ttttttgttc gctcatgtaa tttgcaccgt ctgatgtacg
    14401 tcctttttca gcgtgaatga tttccagggt gtatgcttac aggcaaccaa agggagacag
    14461 actggcctat ggatctcaat acactcatct cacaatatgg ttatgccgcg ctggtgatcg
    14521 gtagcctggc ggaaggtgaa accgtgactt tgctgggagg cgttgcggcg catcaggggc
    14581 tattaaagtt cccgctggtg gtactttctg tggcgcttgg cggcatgatt ggcgaccagg
    14641 tgctttatct gtgcgggcgg cggtttggcg gcaagctgtt acgccgtttc tcgaaacatc
    14701 aggataaaat tgagcgggcg cagaaactta tccaacgcca tccgtatctg tttgtcattg
    14761 gtacgcgctt tatgtatggc tttcgggtga ttggcccgac gctgattggt gccagccagc
    14821 tgccgccgaa aatctttctg ccgcttaata ttctcggcgc atttgcctgg gcgttgattt
    14881 ttaccactat cggttacgct ggtggtcagg tgattgcgcc gtggttgcac aatctcgatc
    14941 agcatttgaa gcactgggtc tggttgattc tggtcgtggt tctggtggta ggcgtgcgct
    15001 ggtggctgaa acgacgcggg aagaaaaagc cggataatca ggcgtaaaac cattgccctg
    15061 gataaggcgt tcgcgccgca tccgacatcc ggtgcaccat gcctgatgcg gcgcttgtgc
    15121 gtcttatcat gcctaccaaa attttaccca ttgaaatatc cacacacctc actgcgctat
    15181 tctgggttga actgcggatt cgccaacata aagccgccat ccactatcaa cgactgcccg
    15241 gtggtgtaat ttgcgccctc cgaacaaagc cacgccacca ggctggcaat ctcatacgtt
    15301 gcgccaaaac gccgcagagg aatcgaaggc tccgcgtcgg gcttcacgtc gctatcatcc
    15361 atgccgttca ttggcgtagc gatcgcccca ggtgcgactg cgttcaccaa aatcttatgc
    15421 ctgaccaact ccagcgccat tgctttggtt aacccgccga gcgcatgttt agcggctgtg
    15481 taggcgctgg catccggcag cggcgtatgt tcatgtaccg acgtaatgtt gatgatgcga
    15541 ccaccctgcc cttgtttcac catctggcgg gccgcaattt gtgagcataa aaatgcacca
    15601 tcgacatcaa cggtaaaaat cttgcgccac tcatcgaaag ccatatcaag aaacggcgct
    15661 ttggtcattg cgcccgcatt attcaccagc acatcaatac gccccagccg ttgaatgagt
    15721 ttctccagcg cctgtgcacc ttctggcagt ttaccgagat ccagctgcac aatctccgca
    15781 cgtacgccgt ggctaactac ctcacgcgcg gtatcttttg ccccttcttc atctgagtgc
    15841 caggtaatgc caatatcaaa cccctgctgc gccagtaata acgcgcactc tttgccgatc
    15901 cccgaatcgg aggcggtaat aatcgcaacc tgtgccatcg agttctccac ttaacgctga
    15961 ataaacgtta agtatagaag gcgcatatca tcagcgtttg taacccccgc ccaacgcacc
    16021 agtgagttga atggaagcat ccagccactg cccttgcaat aacaggccat tggcccgctc
    16081 acgcagcgcg gggattctgg cttcgctgac gcgggaacca gcaatgatgc ccgcgttaaa
    16141 gcgcgcctgc gcaagaccta ccacacgcaa ggcatcgcgc tcaatttgcg cctgatgctg
    16201 gtttttctcc gcaagtgtct gaacctgact ggctgcccgc gccacgtcat tcaccgcttc
    16261 aaccaccgct ttgttgtagc tggcgataga caagttgctt tcggcttttg cgatatcgag
    16321 attggcgtta agacgaccac tatcgaaaat gggtagcgtc aggcctgccg taacgcccat
    16381 ttgctgcgcg gaatgacgga acagatcgct taagtgcaac gcatcctgtt gcaggaaggc
    16441 catcaggttg atgtcaggat aaaatgccgc ttttgccgca tcaatggtgc ttagcgatga
    16501 ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg gccagtaagg agtaccccag
    16561 ttcatcagga agctggcttg ccactttcgg caacgcgacc ggtttaagct tcaatgactt
    16621 tgtctggtta tttgtaagtg cgcttaaccg tgcctcaata attttcattt tccccgcgac
    16681 atcgttgagc tgctgccggg ttttgctggc attaatatcg gtttccacac cttcaactga
    16741 agaagtaatc ccgttctgat atagctggcg atcggtcgcg ataatggtgt tctgctcttt
    16801 ttctatttgc tgcaagaccg tgtttaacgc cgcctgggtt tgccactccc agtacaggcg
    16861 ggctacgctg ccagccagca attggcgggt ttgctcgcgt tccgccgccc gtgctttaac
    16921 cgtacccagg cgggcagtaa cctccgcccg attctttccc cagatatcga gatgccagcc
    16981 cgccgttaag ccaaaagttc cgttggtgta ccacggaccg gtcgtacctg cggccggatc
    17041 gttcagagca aacggcccca ttaagccttc tgccgacatt ttttgccgct ccatatccgc
    17101 agaaaagtcg atctgcggac catcctgagt agcaattgcc ttcgcctggg cttcagctag
    17161 ctgaatgcgc tgttcagcca cctgcatatc cggtgcgttc tgtagtgcat tgttaattaa
    17221 ggaagtgagt tgattatcgt gatactccag ccaccattgg ctgtctggcc aaccattttt
    17281 cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcgggc gtttgctggc ttaacgcctg
    17341 gcgggtttca tgcataggcg cacacccggc cagcatcagt aacagcggaa aacaggcgat
    17401 ggcgggataa aaggaatcac gattcatggg ggaataatca ggtaagaaaa ggtgcgcgga
    17461 gattaccgtg tgttgcgata tattttttag tttcgcgtgg caatacatca gtagcaataa
    17521 aacgacatat ccagaaaaat atacactaag tgaatgatat cttccgattt atcttaatcg
    17581 tttatggata acggcaaagg gcttcgtttt ttcctatact tattcagcac tcacaaataa
    17641 aggaacgcca atgaaaatta tactctgggc tgtattgatt attttcctga ttgggctact
    17701 ggtggtgact ggcgtattta agatgatatt ttaaaaatca gatatggcat ataagttgtc
    17761 aggtcaggaa ctagatattt tgtgtacttg atttgcaaac agctccggtg ttggtggcgt
    17821 caaaatcgct gaagcgttcc ccgaaggccg gggcagccca catggatgtg ggctgagggc
    17881 gcgtcttaca aggacgttac ctcgcgcccg accc
//
LOCUS       DAZGOB010000097        17543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGOB010000097 DAZGOB010000000
VERSION     DAZGOB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.097"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(156..1019)
                     /locus_tag="ACHLIF_003523"
     CDS             complement(156..1019)
                     /locus_tag="ACHLIF_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO9607475.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(991..2541)
                     /locus_tag="ACHLIF_003524"
     CDS             complement(991..2541)
                     /locus_tag="ACHLIF_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO9607476.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            2799..3578
                     /gene="deoC"
                     /locus_tag="ACHLIF_003525"
     CDS             2799..3578
                     /gene="deoC"
                     /locus_tag="ACHLIF_003525"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO9607477.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            3705..5027
                     /gene="deoA"
                     /locus_tag="ACHLIF_003526"
     CDS             3705..5027
                     /gene="deoA"
                     /locus_tag="ACHLIF_003526"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO9607478.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            5079..6302
                     /gene="deoB"
                     /locus_tag="ACHLIF_003527"
     CDS             5079..6302
                     /gene="deoB"
                     /locus_tag="ACHLIF_003527"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO9607479.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            6382..7101
                     /gene="deoD"
                     /locus_tag="ACHLIF_003528"
     CDS             6382..7101
                     /gene="deoD"
                     /locus_tag="ACHLIF_003528"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO9607480.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(7262..7525)
                     /locus_tag="ACHLIF_003529"
     CDS             complement(7262..7525)
                     /locus_tag="ACHLIF_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9607481.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(7557..8573)
                     /gene="lplA"
                     /locus_tag="ACHLIF_003530"
     CDS             complement(7557..8573)
                     /gene="lplA"
                     /locus_tag="ACHLIF_003530"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO9607482.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEVITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(8601..9245)
                     /locus_tag="ACHLIF_003531"
     CDS             complement(8601..9245)
                     /locus_tag="ACHLIF_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO9607483.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            9351..10319
                     /gene="serB"
                     /locus_tag="ACHLIF_003532"
     CDS             9351..10319
                     /gene="serB"
                     /locus_tag="ACHLIF_003532"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO9607484.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            10368..11750
                     /gene="radA"
                     /locus_tag="ACHLIF_003533"
     CDS             10368..11750
                     /gene="radA"
                     /locus_tag="ACHLIF_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO9607485.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            11771..13003
                     /gene="nadR"
                     /locus_tag="ACHLIF_003534"
     CDS             11771..13003
                     /gene="nadR"
                     /locus_tag="ACHLIF_003534"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO9607486.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13310..14977)
                     /gene="ettA"
                     /locus_tag="ACHLIF_003535"
     CDS             complement(13310..14977)
                     /gene="ettA"
                     /locus_tag="ACHLIF_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO9607487.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            15188..17125
                     /gene="sltY"
                     /locus_tag="ACHLIF_003536"
     CDS             15188..17125
                     /gene="sltY"
                     /locus_tag="ACHLIF_003536"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO9607488.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     HIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSTTEWGRRY"
     gene            17215..17541
                     /gene="trpR"
                     /locus_tag="ACHLIF_003537"
     CDS             17215..17541
                     /gene="trpR"
                     /locus_tag="ACHLIF_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO9607489.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
BASE COUNT         4017 a         4574 c         4892 g         4060 t
ORIGIN      
        1 cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg ccggataagg cgttcacgcc
       61 gcatccggca gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta tcatgcctac
      121 aagtctgtgc cgaaccgtag gccgtatccg gcatgtcaca aatagagcgc cggaaatatc
      181 aaccggctca ccccgcgcac ctttaacgca tcagccaacg gctcaacgtc ttccggcgtg
      241 gcgctcgccc agctttgcgc ctcgccatac acgccgtggg catgaaacgc gttcaggcgt
      301 accggaacat cgccaagtcc cttgataaac accgccagtt cttcgatgtg ttgcaaataa
      361 tccacctggc cagggattac cagcaaacgc agttccgcca gcttaccgcg ctctgccagc
      421 aaacagatgc tgcgcttaat ctgctgatta tcgcgtccgg ttaattgttg atgacattcg
      481 ctcccccacg ctttgagatc gagcattgcg ccgtcacaca ccgggagtaa tttttcccag
      541 ccggtttcgc tcaacatacc gttactgtcc acgaggcagg tgaggtggcg cagttgcgga
      601 tcgtttttga tggctgtaaa cagcgccacc acaaacggca gctgggtcgt ggcttcgccg
      661 ccgcttaccg ttatcccttc gataaacagc accgctttgc ggatatggct aagcacctca
      721 tccacgctca tggattgcgc cattggcgtg gcatgttgcg gacacatctt caggcaggta
      781 tcacactgct cgcagaccac agcgttccac agcactttgc cgtcaacaat ctgcaacgcc
      841 tgatgcggac actgtggcac gcactctccg caatcattgc agcgtcccat cgtccacgga
      901 ttgtgacagt ttttgcagcg cagattgcag ccctgcaaaa acagagccag acgactgcct
      961 ggcccgtcaa cgcaggagaa ggggataatc ttactgacta aagcgcatct gctgttcatg
     1021 gcttatcacg cgcggctggc gttccagaat acgagtgttg cgtgcggctt cttcgcccag
     1081 ccaggtggtg ttggtgcgtg aaccttcggc gcgatatttt tctaaatccg acaaacgcac
     1141 catataaccg gtaacgcgaa ccagatcgtt accgctgaca ttggcggtaa attcacgcat
     1201 tccggcttta aaggcaccga ggcaaagctg taccagtgcc tgcgggttac gtttgatggt
     1261 ttcgtcgagg gtcagaatgt cgctgatgcc ggaataataa taagcatgat gcggggcgac
     1321 agtttgcaga tgggtgatcg gatctggctc atcgccatac ggcaaacgcg cgcccggcgt
     1381 ggtgccgata tcggaactga tccccgactg tgcgtgtaac atggcgcgtt tttgccagcc
     1441 atatttcacg ggggtattgg cgacaaactc cgccagttgc gcgctgatgc gataacccac
     1501 ttcatttgcg gcggcttctt taccgtagcg cgcggcaatc ccttctttct cacacagcaa
     1561 gttaaccgct tccgccagcc catacatgcc aaacattggc acaaaacgtt cagggttaat
     1621 cagcccttct ttcaccagga agctattctc aaagaagtgt gattgttgat agaggaattc
     1681 acaccgcgca tcgatgatgg cgatctgctg ctggcagtag tgcggtagag tgcgcgtaaa
     1741 gaagtcatcc agcgattcgc tgcgctcggc aatggctttc aggttaaggc gtaccagcgt
     1801 gctgccacca cccgccagcg gcagtgagtt gtaacagctc acaatcccgt agcccccttt
     1861 tgtgaaaatt ttatcatgca ccggaccgtt ggcgatgtgc ggtttgctac attcacagat
     1921 gttcttcgcc acttccagca gcaggtcatc aggggtgatt tctggatcgt agataaaggt
     1981 caggttcggt gaaacctgct tcaactctgc atctgcacgt aatatcgcac gggtgatggg
     2041 cgaatcagac gggccgatat tggcgtgcat aaaggcgtct ggcagggttc tgtcgaggta
     2101 acgccagaaa cgttttattc gaatatcgat ctcgtcttgt gttagaattc taacatacgg
     2161 ttgcaacaac gcatccagtt gccccaggta gaccggcatc gatgtgaccg acggtacgtg
     2221 gtggtaaaga atggtcagca gagagagtgc gtcatcaaga tctttcgcgc cttccagctc
     2281 cagccattcg gaaccgttcg ccagaaaacg ggcgtaatcg ggtaagacat agcgcggttt
     2341 gtacggcgca tgaccttcaa acatatcgca gattacaccc tcatccagcg cgcggcgggc
     2401 ctcggcaggc agctgcggat aaggcagatt gttttctgct tccagtgcca gaaaatggcg
     2461 cttctgctcc gggctaagca ctgggctggt gacaatttgc tggcaacgtt gttgcagtgc
     2521 attttcatga gaagtgggca tcttcttttc cttttatgcc gaaggtgatg cgccattgta
     2581 agaagtttcg tgatgttcac tttgatcctg atgcgtttgc caccactgac gcattcattt
     2641 gaaagtgaat tatttgaacc agatcgcatt acagtgatgc aaacttgtaa gtagatttcc
     2701 ttaattgtga tgtatatcga agtgtgttgc ggagtagatg ttagaatact aacaaactcg
     2761 caaggtgaat tttattggcg acaagccagg agaatgaaat gactgatctg aaagcaagca
     2821 gcctgcgtgc actgaaatta atggacctga ccaccctgaa tgacgacgac accgacgaga
     2881 aagtaattgc tctgtgtcat caggccaaaa ccccggtcgg caataccgcc gctatctgta
     2941 tctatcctcg ctttatcccg attgctcgca aaacgctgaa agagcagggc accccggaaa
     3001 tccgaatcgc tacggtaacc aacttcccac acggtaacga tgacatcgaa atcgcgctgg
     3061 cagaaacccg tgcggcaatc gcctacggtg cagatgaagt tgacgttgtg ttcccgtacc
     3121 gcgcgctgat ggcgggcaac gagcaggttg gttttgacct ggtgaaagcc tgtaaagagg
     3181 cttgcgcggc agcgaatgta ctgctgaaag tgatcatcga aaccggcgaa ctgaaagacg
     3241 aagcgctgat ccgtaaagcg tctgaaatct ccatcaaagc gggtgcggac ttcatcaaaa
     3301 cctctaccgg taaagtggct gtgaacgcga cgccggaaag cgcgcgcatc atgatggaag
     3361 tgatccgtga tatgggcgta gaaaaaaccg ttggtttcaa accggcgggc ggcgtgcgta
     3421 ctgcggaaga tgcgcagaaa tatctcgcca tcgcagatga actgtttggt gctgactggg
     3481 ctgatgcgcg tcactaccgc tttggtgctt ccagcctgct ggcaagcctg ctgaaagcgc
     3541 tgggtcacgg cgacggtaag agcgccagca gctactaagt aagatgcttt acgcctgatg
     3601 cgctgcgctt atcaggccta cgagacgtat ctacccgtag gccggataag gcgtagacgc
     3661 atccggcaaa agccgcctca tactcttttc ctcgggaggt taccttgttt ctcgcacaag
     3721 aaattattcg taaaaaacgt gatggtcatg cgctgagcga tgaagaaatt cgtttcttta
     3781 tcaacggtat tcgcgacaac actatctccg aagggcagat tgccgccctc gcgatgacca
     3841 ttttcttcca cgatatgaca atgcctgagc gtgtctcgct gaccatggcg atgcgagatt
     3901 caggaaccgt tctcgactgg aaaagcctgc atctgaatgg cccgattgtt gataaacact
     3961 cgaccggcgg cgtcggcgat gtgacttcgc tgatgttggg gccgatggtc gcagcctgcg
     4021 gcggctatat tccgatgatc tctggtcgcg gcctcggtca tactggcggt acgctcgaca
     4081 aactggaatc catccctggc ttcgacattt tcccggatga caaccgtttc cgcgaaatta
     4141 ttaaagacgt cggcgtggcg attatcggtc agaccagctc actggctccg gcggataaac
     4201 gtttctacgc gacccgtgat attaccgcaa ccgtggactc tatcccgctg atcactgcct
     4261 cgatcctggc gaagaaactg gcggaaggtc tggatgcgct ggtgatggac gtgaaagtgg
     4321 gtagcggcgc gtttatgccg acctacgaac tctctgaagc ccttgccgaa gcgattgttg
     4381 gcgtggctaa cggcgctggc gtgcgcacca ccgcgctgct caccgacatg aatcaggtac
     4441 tggcctccag tgcaggtaac gcggttgaag ttcgtgaagc ggtgcagttc ctgacgggtg
     4501 agtatcgtaa cccgcgtctg tttgatgtca cgatggcgct gtgcgtggag atgctgatct
     4561 ccggcaaact ggcgaaagat gacgccgaag cgcgcgcgaa attgcaggcg gtgctggaca
     4621 acggtaaagc ggcagaagtc tttggtcgta tggtagcggc acaaaaaggc ccgaccgact
     4681 tcgttgagaa ctacgcgaag tatctgccga cagcgatgct gacgaaagca gtctatgctg
     4741 ataccgaagg ttttgtcagt gaaatggata cccgcgcgct ggggatggca gtggttgcaa
     4801 tgggcggtgg acgccgtcag gcatctgaca ccatcgatta cagcgtcggc tttactgata
     4861 tggcgcgtct gggcgaccag gtagacggtc agcgtccgct ggcggttatc cacgcgaaag
     4921 acgaaaacag ctggcaggaa gcggcgaaag cggtgaaagc ggcaattaaa cttgccgata
     4981 aagcaccgga aagcacacca actgtctatc gtcgtatcag tgaataacgg tatactgatc
     5041 tgatcattta aatttgaagc actgagtacg gagaacatat gaaacgtgca tttattatgg
     5101 tgctggactc attcggcatc ggcgctacag aagatgcaga acgctttggt gacgtcgggg
     5161 ctgacaccct gggtcatatc gcagaagcct gtgccaaagg cgaagctgat aacggtcgta
     5221 aaggcccgct caatctgcca aatctgaccc gtctggggct ggcgaaagca cacgaaggtt
     5281 ctaccggttt cattccggcg ggaatggacg gcaacgctga agttatcggc gcgtacgcat
     5341 gggcgcacga aatgtcatcc ggtaaagata ccccgtctgg tcactgggaa atcgccggtg
     5401 tcccggttct gtttgagtgg ggatatttct ctgatcacga aaacagcttc ccgcaagagc
     5461 tgctggataa actggtcgaa cgcgctaatc tgccgggtta cctcggtaac tgccactctt
     5521 ccggtacggt cattctggat cagctgggcg aagagcacat gaaaaccggc aagccgattt
     5581 tctatacctc cgctgactct gtgttccaga ttgcctgcca cgaagaaacc tttggcctgg
     5641 ataaactcta cgaactgtgc gaaatcgccc gtgaagagct gaccaacggc ggctacaaca
     5701 tcggtcgtgt tatcgctcgt ccgtttatcg gtgacaaagc cggtaacttc cagcgtaccg
     5761 gtaaccgtca cgacctggct gttgagccgc cagcaccgac cgtgctgcag aaactggttg
     5821 atgaaaaaca cggtcaggtg gtttctgtcg gtaaaattgc cgacatctac gccaactgcg
     5881 gtatcaccaa gaaagtgaaa gcaactggcc tggacgcgct gtttgacgcc accatcaaag
     5941 agatgaaaga agcgggtgat aacactatcg tcttcaccaa cttcgttgac ttcgactctt
     6001 cttggggcca ccgtcgcgac gtcgccggtt atgctgcggg tctggagctg ttcgaccgcc
     6061 gtctgccgga gctgatgtct ctgctgcgcg atgacgacat cctgatcctc actgctgacc
     6121 acggttgtga tccgacctgg accggtactg accacacgcg tgaacacatt ccggtactgg
     6181 tttacggccc gaaagtaaaa ccgggctcac tgggtcaccg tgaaaccttc gcggatatcg
     6241 gccagactct ggcaaaatat tttggtactt ctgatatgga atatggcaaa gccatgttct
     6301 gataatgggt gcggcttgat gccctcaccc cggccctctc ccacagggag agggtgaaaa
     6361 catgtagata aggataaaac aatggctacc ccacacatta atgcagaaat gggcgatttc
     6421 gctgacgtag ttttgatgcc aggcgacccg ctgcgtgcaa agtatattgc tgaaactttc
     6481 cttgaagatg cccgtgaagt gaacaacgtt cgcggtatgc tgggcttcac cggtacttac
     6541 aaaggccgca aaatttccgt aatgggtcac ggtatgggta tcccgtcctg ctccatctac
     6601 accaaagaac tgatcaccga tttcggcgtg aagaaaatta tccgcgtggg ttcctgtggc
     6661 gcagttctgc cgcacgtaaa actgcgcgac gtcgttatcg gtatgggtgc ctgcaccgat
     6721 tccaaagtta accgcatccg ttttaaagat catgactttg ccgctatcgc tgactttgac
     6781 atggtgcgta acgcggtaga cgcggctaaa gcactgggcg ttgatgctcg cgtgggtaac
     6841 ctgttctccg ctgacctgtt ctactctccg gacggcgaaa tgttcgacgt gatggaaaaa
     6901 tacggcatcc tcggcgtgga aatggaagcg gctggtatct acggcgtggc tgcagagttt
     6961 ggcgcgaaag ccctgaccat ctgcaccgtg tctgaccaca tccgcactca cgagcagacc
     7021 actgccgctg agcgtcagac caccttcaac gacatgatca aaatcgcact ggaatccgtt
     7081 ctgctgggcg ataaagagta attgtgtttc gctgaaaggc gaacgtcgtg ttaagccgga
     7141 gcaggagact gctccggctt tttagtttct attcatcaat agcaactgat ttatagattt
     7201 actattattt ttcatatgga tagaaaataa aatccattca agtatctatt cacgaatcta
     7261 ttcatttatg ttcagccgtc atccaggaag ggcatatcag caactctggt ggacaaccat
     7321 aaatttctgc cattttgctt ttgatgtttg agctaagccg ttccgtcttt tcgatctgtt
     7381 ttagagaatt aatcgagata cctaacattt tacttacttc agcaagcgat aatttatgcc
     7441 tgatacgcac ggcggctagc caggaaatac gctggcttaa cattaaattg ataatctccc
     7501 gctcctggtt ttccagatcg atcatattcc cttcctttgg tattttaatc agcgagttac
     7561 cttaccgccc ccgctatcca cgtcgacaac tcccgtagct ctttttcctg gtccgggaag
     7621 tcaaccaaca gcgcttcgca ctcttgttgc agcatatccg cgcggtacag gcagccttgc
     7681 agtcgcccgg cgagggcttc cagcggcgcg gggttgaggc tgtcggtaaa cacctgggcg
     7741 cgggtgatat ggcctttttc aacgtcgaaa tgcagttcca cgccgcccca gctaaagcgt
     7801 tcatccagca gatgcgagaa tgccggagcc tgaccgaagt tccattccca gctactctga
     7861 cgggcaaagg tttcggcgaa gtttggcaag tctggcgttt tgtccgggga gatgatttcc
     7921 gcttctacac gctcgccata atgggcgaaa aaggccttgg ttatgacctc gcaaacctgc
     7981 tcatgggtga tccccggcag cagctcggtg aggttggtca cgcgggaacg cactgaggta
     8041 atgcctttcg ccgccagttt ctttttatcc ggattgagat agtttgccag gcggctaagg
     8101 tcggcattga gcagcaaagt gccgtggtgg aagccacgat ctttggtttc gcgataggcc
     8161 gagcctgaga ctttgcggtc gccttcgact gttttcacca ccagatcgtt acgcccggac
     8221 gcttcggcgc tgacgccgag cgcgttcagc gcattgagca caatcgacgt agagatggtt
     8281 ttatcgtact ccggcttgcc agccataaag gtaaagcagg tattgccgag atcgtggaac
     8341 accgcgccgc caccgctact gcgccgcgcc aggcggacgt tatcttcttc catccgccgg
     8401 gtattacact ctttccacgg gttctgcgcg cgaccaatca ctaccgtgtc ggcattgcgc
     8461 cagagaaaca gaacgcgctg cgtggcgggc atttggcgaa aaatacactc ttccaccgcc
     8521 aggttaaacc acgggtcgta agagtcagag atgagcaggc gtaatgtgga cataacgatt
     8581 tcctttcttg tagtaatggg tcactctttt ttctcgcttt cttcctcttc cggcaccggt
     8641 ttgctggcgg ttaacaggaa gggcgattgc tgccagcggg tgcgtttacc ctgtagcagc
     8701 gtgcgggtca gcactacgcc gattgccagt gagagcagca acatcaggcg taaaatgtta
     8761 gtggtgttat ccacctgttg ggcttcggtg gcgagcgtat gggtgtcgag tgtcaggcgc
     8821 agatagccga gcggtccgtt tttacccgca attggctcga caatctgctg gttaaaatag
     8881 ccgcctgctt ttttaccgtc gagcgccagc cggtcgcgca cttcgacgct ttcgcctgaa
     8941 cgggcgataa gatcgccttg ttcgtcatac acacccgcgt cgaggatgcg gctttcatcc
     9001 gttaactgat cgaggatcgc ctgaatgcgt ttttcatctg gtgagtcggt acgcatcagc
     9061 ggtgcaacgt tcagcgtcac ctgacgcgcc agggtgcggg ccagttcttc cagctgtgga
     9121 ttacgctgtc gctggtggtt ttgactaaac catgacgccc cctgcatcag tgccactaac
     9181 aaggcaagac agaacaggac aatcactgcc cgatgcagcc ggaatttcag ttttgtgcga
     9241 gccatcttcc accctttgaa aatttgagac ttaatgttgc cagaagcaat ggatacaagg
     9301 tagcctcatg cgttattttc cctgcttcga acgattttac aggagcctta atgcctaaca
     9361 ttacctggtg cgacctgcct gaagatgtct ctttatggcc gggtctgcct ctttcattaa
     9421 gtggtgatga agtgatgcca ctggattacc acgcaggtcg tagcggctgg ctgctgtatg
     9481 gtcgtgggct ggataaacaa cgcctgaccc aataccagag caaactgggc gcggcgatgg
     9541 tgattgttgc cgcctggtgc gtggaagatt atcaggtgat tcgtctggca ggttcactca
     9601 ccgcgcgcgc tacgcgcctg gcccatgaag cgcaactgga tgtcgcgccg ctggggaaaa
     9661 tcccgcacct gcgcacgccg ggtttgctgg tgatggacat ggattccacc gccatccaga
     9721 ttgaatgtat tgatgaaatt gccaaactgg ccggaacggg cgagatggtg gcggaggtaa
     9781 ccgaacgggc gatgcgcggc gaactcgatt ttaccgccag cctgcgcagc cgcgtggcga
     9841 cgctgaaagg cgctgatgcc aatattctgc aacaggtgcg tgaaaatctg ccgctgatgc
     9901 caggcttaac gcagctggtg ctcaagctgg aaacgctggg ctggaaagtg gcgattgcct
     9961 ccggcggctt tactttcttt gctgaatacc tgcgcgacaa gctgcgcctg accgccgtgg
    10021 tagccaatga actggagatc atggacggta aatttaccgg caatgtgatc ggcgacatcg
    10081 ttgacgcgca gtacaaagcg aaaactctga cccgcctcgc gcaggagtat gaaatcccgc
    10141 tggcgcagac cgtggcgatt ggcgatggag ccaatgacct gccgatgatc aaagcggcag
    10201 ggctggggat tgcctaccat gccaagccaa aagtgaatga aaagacggaa gtcaccatcc
    10261 gtcacgctga cctgatgggg gtattctgca tcctctctgg cagcctgaat cagaagtaat
    10321 tgcctgcccg tcatcctgcg ggcggcacag cattaacgag gtacaccgtg gcaaaagctc
    10381 caaaacgcgc ctttgtttgt aatgaatgcg gggccgatta tccgcgctgg caggggcagt
    10441 gcagtgcctg tcatgcctgg aacaccatca ccgaggtgcg tcttgctgcg tcgccaacgg
    10501 tggcgcgtaa cgagcgtctc agtggctatg ccggtagcgc cggggtggca aaagtccaga
    10561 aactctccga catcagcctt gaagagctgc cgcgtttttc caccggattt aaagagttcg
    10621 accgcgtatt aggcggcggc gtggtaccgg gaagtgccat tctgattggc ggtaacccag
    10681 gcgcggggaa atccacgctg ttgctgcaaa cgctgtgcaa actggcccag cagatgaaaa
    10741 cgctgtatgt caccggcgaa gagtcgctgc aacaggtggc aatgcgcgct catcgccttg
    10801 gcctgccgac tgacaatctc aatatgttgt cggaaaccag catcgagcag atctgcctga
    10861 ttgctgaaga agagcaaccg aagctgatgg taattgactc catccaggtg atgcatatgg
    10921 cggatgtaca gtcatcgcct ggtagcgtgg cgcaggtgcg tgaaacggcg gcttatttga
    10981 cacgcttcgc caaaacgcgc ggtgtggcga ttgtcatggt tggacacgta accaaagatg
    11041 gctcgctggc gggccctaaa gtgctggaac actgtatcga ctgttcggtg cttttggatg
    11101 gcgatgccga ctcccgtttt cgcaccttgc gcagccataa aaaccgcttc ggcgcggtga
    11161 atgagctggg cgtcttcgcg atgaccgaac aggggctgcg tgaagtcagc aacccttcgg
    11221 caattttctt aagtcgcgga gatgaagtga cctccggcag ctccgtgatg gtggtgtggg
    11281 aaggaacgcg tccgttgctg gtggagattc aggcgctggt cgatcactcg atgatggcga
    11341 atccgcgccg cgtggcggta ggtctggagc aaaaccgtct ggcaatcctg ctggctgtct
    11401 tgcaccgtca cggtggtctg caaatggccg atcaggatgt atttgtgaac gtggtcggtg
    11461 gtgtgaaggt aaccgaaacc agtgccgact tagcgttact gctggcgatg gtttccagcc
    11521 tgcgtgacag accgctaccg caggatctgg tggtgtttgg tgaagtcggg ctggcagggg
    11581 agatccgccc ggtgcccagc ggtcaggagc gaatctctga agcggcgaaa cacggttttc
    11641 gccgggcgat tgttccggcg gctaacgtgc cgaaaaaagc gccggaaggg atgcagattt
    11701 ttggcgttaa aaaactctcc gacgcgctta gcgtgttcga cgacttataa tgagagatac
    11761 ggagggagat atgtcgtcat ttgattacct gaaaactgcc atcaagcaac agggctgcac
    11821 gttacagcag gtagctgatg ccagcggtat gaccaaaggg tatttaagcc agttactgaa
    11881 tgccaaaatt aaaagcccca gcgcgcaaaa gctggaggcg ttgcaccgtt ttttggggct
    11941 tgaatttccc cggcagaaga aaaccattgg tgtcgtattc ggtaagttct acccgctgca
    12001 taccggacat atctacctta tccagcgcgc ctgtagccag gttgacgagc tgcatatcat
    12061 tatgggtttt gacgataccc gcgaccgcgc gttgtttgaa gacagcgcca tgtcgcagca
    12121 gcccacggtg ccggatcgtc tgcgctggtt attgcaaact tttaaatatc agaaaaatat
    12181 tcgcattcat gctttcaacg aagagggcat ggagccgtat ccgcatggct gggatgtgtg
    12241 gagcaacggc atcaaaaagt ttatggctga aaaggggatc cagccggacc tgatctacac
    12301 ctcggaagaa gccgatgcgc cacagtatat ggaacatctg gggatcgaga cggtactggt
    12361 cgatccgaaa cgtaccttta tgagtatcag cggtgcgcag atccgcgaaa acccgttccg
    12421 ctactgggaa tatattccta cggaagtgaa gccgttcttt gtgcgtaccg tggcgatcct
    12481 tggcggcgag tcgagcggta aatccaccct ggtaaacaaa cttgccaata tcttcaatac
    12541 caccagtgcg tgggaatatg gtcgcgatta tgtcttttca cacctcggcg gtgatgagat
    12601 cgcattgcag tattccgact acgataaaat cgcgctgggc cacgcacaat acattgattt
    12661 tgcagtgaaa tacgccaata aagtggcgtt tattgacact gattttgtca ctacccaggc
    12721 gttctgcaaa aagtacgaag ggcgtgagca tccgttcgtg caggcgttga ttgatgaata
    12781 ccgtttcgat ctggtgatcc tgctggagaa caacacgccg tgggtggcgg atggtttacg
    12841 cagcctcggc agttcggtgg atcgcaaaga gttccagaac ttgctggtgg agatgctgga
    12901 agagaacaat atcgaattcg tgcgggttga agaggacgat tatgacagcc gtttcctgcg
    12961 ctgcgtggag ctggtgcggg agatgatggg ggagcagaga taaccgcgat gaaacggctc
    13021 aaaggcgagg tataaaataa gttttttgat gagttgaaaa cctttctgga agaggctgtc
    13081 gagattaaac aaggtttgaa aaaacctgca cgggtgaccc gctacgaaat tgcggatact
    13141 aaggctgttg taggccggaa aaggcgttca cgccgtatcc ggcattcggt gctcaatgcc
    13201 tgatgcgacg cttaccgcgt cttatcaggc ctggagattc acaacgtatt gaaatttctt
    13261 ggtttgtagg ccggataagg cgttcacgcc gcatccggca ttttacgcat tacttcgcaa
    13321 tacgcttgta cttgatacgc ttcggctcca gtgcgtctgc gcccagcgtg cgtttcttgt
    13381 actcttcgta ctcagtaaag ttaccttcga agaactcaac tttaccttca tcctggtagt
    13441 ccaggatgtg cgtggcgata cggtcgagga accaacggtc gtgcgagata accatcgcac
    13501 agcccgggaa ctccagcagg gcgttttcca gcgcgcgcag ggtttcgata tccaggtcgt
    13561 tggttggttc gtcgagcagc agcatgttgc cgccaacctg cagcagcttc gccagatgca
    13621 gacgaccgcg ctcaccaccg gaaagttcac caacgcgttt accctgatca acccctttaa
    13681 agttaaagcg gccaacgtag gcgcggcttg gcatctcggt gttgccgatc ttcataatat
    13741 ccagcccgcc ggaaacttct tcccaaacgg ttttgctgtt atccattgag tcacggaact
    13801 gatcaaccga tgccagtttc accgtttcac ctaaagtgat ggtgccgctg tccggctgtt
    13861 cctgaccaga gatcatacgg aacagggtcg atttacccgc gccgttcgga ccgatgatcc
    13921 cgacgattgc ccctttcggg atcgagaagc tcaggtcatc aatcagcagg cgatcaccgt
    13981 aggatttacg caggttgctg acttccagca ctttatcgcc cagacgcggt ccaggtggaa
    14041 taaacagttc gttggtttcg ttacgtttct gatattcggt gctgttcagc tcttcaaagc
    14101 gtgccagacg tgctttacct ttcgactggc ggcctttagt tccctgacgt acccactcca
    14161 gctctttctc gatcgactta cgacgcgccg cttcttgtga agcttcctgc gccaggcgct
    14221 gatctttctg ctccagccag gaggagtagt tgccttccca cggaatacct tcaccgcggt
    14281 caagttcgag gatccagcct gcaacgttat cgaggaagta acggtcgtgg gtaatcgcca
    14341 ccacggtgcc ctcgaagtcg tgcaggaagc gttccagcca ggccacggat tcggcatcca
    14401 ggtggttggt cggttcgtcg agcagcagca tgtctggttt ttccagcagc aggcggcaca
    14461 acgctacgcg acgacgctca ccaccggaga ggttagcgat tttcgcgtcc cagtccggca
    14521 gacgtagcgc atccgccgca cgctccagct gtacgttcag gttatgaccg tcgtgagcct
    14581 gaatgatctc ttccagacgg ccttgttcag cggccagctt gtcaaaatcg gcatccggat
    14641 cggcgtacag cgcatacact tcatccaggc gtttcagggc gttaaccact tcagaaaccg
    14701 cttcttcaat ggactcacgc acggtgtgtt ccgggttcag ctgcggttcc tgcggcaggt
    14761 aaccaatctt gatgtctggc tgcggacgcg cttcaccttc gatgtcttta tcaatgcccg
    14821 ccataatgcg cagcagggta gacttacccg cgccgttcag acccaggaca ccaatttttg
    14881 ccccagggaa gaaactcaga gagatgtttt tcaaaatatg acgtttcggc ggaacaactt
    14941 tgccgacacg atgcatggta taaacgaatt gagccacgtt ggacttcgcc tctatgttta
    15001 tcgtgatgat gagttttcaa aggcgaagtg tagccttttt ccccgcctaa tcccagttgg
    15061 tcgatcacct tcgcagtaaa agtaaaaaag tgtccgtaac gtggcgtaaa cggcaatgac
    15121 tggttagcat aaatctatta cgcggcatga cgctgcattg atgtatttac acttagagga
    15181 tgcgcttgtg gaaaaagcca aacaagttac ctggcggctg ttggctgccg gtgtctgtct
    15241 gctgacggtc agcagcgtgg cgcgagccga ctcactggat gagcagcgta gccgttacgc
    15301 gcaaatcaag caggcctggg ataatcgaca aatggatgtg gtcgaacaaa tgatgcctgg
    15361 actgaaggat tatccgcttt atccctacct ggaataccgc cagatcaccg atgatctgat
    15421 gaatcaaccg gcggtgacgg tcactaactt tgttcgcgct aaccccacgc ttcctcccgc
    15481 tcgcacgctg caatctcgtt tcgtcaatga actggcgcgg cgtgaagact ggcgtggctt
    15541 gttagccttt agcccggaaa agcccggaac taccgaagcg caatgtaatt actactatgc
    15601 gaaatggaac accgggcaga gtgaagaagc ctggcaaggg gcgaaagagc tgtggctaac
    15661 cggcaagagc cagcctaacg cctgtgacaa gttatttagc gtctggcgtg cgtcaggtaa
    15721 acaagatccg ctggcttatt tagagcgtat ccgtctggcg atgaaagcgg gtaacactgg
    15781 cctggtaaca gtgctggcag ggcagatgcc tgccgattac cagactatcg cctcggcaat
    15841 catttcactg gcgaacaacc ctaatacggt actgaccttc gcgcgtacaa ccggagcgac
    15901 cgattttacc cgtcaaatgg cggcggtggc gtttgccagt gtggcgcggc aggatgctga
    15961 aaatgcacgg ctgatgatcc cgtcgcttgc ccaggcgcag cagcttaatg aagatcatat
    16021 tcaggagctg cgcgatatcg tcgcctggcg tttgatgggc aacgatgtca ccgacgagca
    16081 ggcgaaatgg cgcgatgacg ccattatgcg ctcgcaatcc acgtcgttga ttgaacgccg
    16141 tgtgcgaatg gcacttggca ccggcgatcg tcgcggcctg aatacctggc tggcgcgtct
    16201 gccgatggaa gcaaaagaga aagatgaatg gcgttactgg caggcggatt tattgctgga
    16261 acgcggacgt gaagctgaag cgaaagagat tttgcatcaa ctcatgcaac agcgtggttt
    16321 ctacccgatg gttgcagcac aacgcatcgg agaagagtat gagctgaaga ttgataaagc
    16381 gccgcagaat gttgacagcg ccctgactca ggggccggag atggcgcgcg tgcgcgagtt
    16441 gatgtactgg aatctcgata acaccgcgcg tagcgagtgg gccaatctgg tgaagagcaa
    16501 gtcaaaaaca gagcaggctc aactggctcg gtatgctttc aataaccaat ggtgggatct
    16561 tagcgttcag gcaacgatcg ccgggaagct ttgggatcat ctggaagagc gattcccgct
    16621 ggcttacaac gatcttttca aacgttacac cagtgggaaa gagatcccgc aaagctatgc
    16681 gatggcgatt gcccgtcagg agagcgcctg gaatccgaaa gtgaaatcac cggtaggggc
    16741 cagcggcctg atgcagatta tgcctggtac agcgacccat acggtgaaga tgttctctat
    16801 tcctggttat agcagccctg ggcaattgct ggatccggaa acgaatatca acattggcac
    16861 cagttacctg caatatgttt atcagcagtt tggcaataac cgtattttct cctcagcagc
    16921 ttataacgcc ggacctggac gggtgcgaac ctggcttggc aacagcgccg ggcgtatcga
    16981 cgctgtggca tttgtcgaga gtattccgtt ctccgagacg cgcggttatg tgaagaacgt
    17041 gctggcttat gacgcttact accgctattt catgggggat aaaccgacgt tgatgagcac
    17101 cacggaatgg ggacgtcgtt actgatccgc acgtttatga tatgctatcg tactctttag
    17161 cgagtacaac cgggggaggc attttgcttc ccccgctaac aatggcgaca tattatggcc
    17221 caacaatcac cctattcagc agcgatggca gaacagcgtc accaggagtg gttacgtttt
    17281 gtcgacctgc ttaagaatgc ctaccaaaac gatctccatt taccgttgtt aaacctgatg
    17341 ctgacgccag atgagcgcga agcgttgggg actcgcgtgc gtattgtcga agagctgttg
    17401 cgcggcgaaa tgagccagcg tgagttaaaa aatgaactcg gcgcgggcat cgcgacgatt
    17461 acgcgtggat ctaacagcct gaaagccgcg cccgttgagc tgcgccagtg gctggaagag
    17521 gtgttgctga aaagcgattg att
//
LOCUS       DAZGOB010000098        17523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGOB010000098 DAZGOB010000000
VERSION     DAZGOB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.098"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            134..388
                     /gene="bssS"
                     /locus_tag="ACHLIF_003538"
     CDS             134..388
                     /gene="bssS"
                     /locus_tag="ACHLIF_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO9607490.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSELEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            503..1621
                     /gene="solA"
                     /locus_tag="ACHLIF_003539"
     CDS             503..1621
                     /gene="solA"
                     /locus_tag="ACHLIF_003539"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO9607491.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTVIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1669..1782
                     /locus_tag="ACHLIF_003540"
     CDS             1669..1782
                     /locus_tag="ACHLIF_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO9607492.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2043..2609
                     /gene="yceJ"
                     /locus_tag="ACHLIF_003541"
     CDS             2043..2609
                     /gene="yceJ"
                     /locus_tag="ACHLIF_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO9607493.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2613..3188
                     /gene="yceI"
                     /locus_tag="ACHLIF_003542"
     CDS             2613..3188
                     /gene="yceI"
                     /locus_tag="ACHLIF_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO9607494.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3230..4282)
                     /gene="trhO"
                     /locus_tag="ACHLIF_003543"
     CDS             complement(3230..4282)
                     /gene="trhO"
                     /locus_tag="ACHLIF_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO9607495.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4507..5427
                     /gene="lpxL"
                     /locus_tag="ACHLIF_003544"
     CDS             4507..5427
                     /gene="lpxL"
                     /locus_tag="ACHLIF_003544"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO9607496.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPQQYMWLHRRFKTRPEGVPSRY"
     gene            5599..6825
                     /gene="mdtG"
                     /locus_tag="ACHLIF_003545"
     CDS             5599..6825
                     /gene="mdtG"
                     /locus_tag="ACHLIF_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO9607497.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6908..7282
                     /gene="msyB"
                     /locus_tag="ACHLIF_003546"
     CDS             6908..7282
                     /gene="msyB"
                     /locus_tag="ACHLIF_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO9607498.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7283..7510)
                     /gene="yceK"
                     /locus_tag="ACHLIF_003547"
     CDS             complement(7283..7510)
                     /gene="yceK"
                     /locus_tag="ACHLIF_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO9607499.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7683..10226)
                     /gene="mdoH"
                     /locus_tag="ACHLIF_003548"
     CDS             complement(7683..10226)
                     /gene="mdoH"
                     /locus_tag="ACHLIF_003548"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO9607500.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10219..11754)
                     /gene="mdoG"
                     /locus_tag="ACHLIF_003549"
     CDS             complement(10219..11754)
                     /gene="mdoG"
                     /locus_tag="ACHLIF_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO9607501.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12148..13305
                     /gene="mdoC"
                     /locus_tag="ACHLIF_003550"
     CDS             12148..13305
                     /gene="mdoC"
                     /locus_tag="ACHLIF_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO9607502.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13313..14794)
                     /gene="clsC"
                     /locus_tag="ACHLIF_003551"
     CDS             complement(13313..14794)
                     /gene="clsC"
                     /locus_tag="ACHLIF_003551"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO9607503.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14736..15269)
                     /gene="ymdB"
                     /locus_tag="ACHLIF_003552"
     CDS             complement(14736..15269)
                     /gene="ymdB"
                     /locus_tag="ACHLIF_003552"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO9607504.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15364..15675)
                     /gene="ymdA"
                     /locus_tag="ACHLIF_003553"
     CDS             complement(15364..15675)
                     /gene="ymdA"
                     /locus_tag="ACHLIF_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO9607505.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15796..16128)
                     /gene="csgC"
                     /locus_tag="ACHLIF_003554"
     CDS             complement(15796..16128)
                     /gene="csgC"
                     /locus_tag="ACHLIF_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO9607506.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16187..16642)
                     /gene="csgA"
                     /locus_tag="ACHLIF_003555"
     CDS             complement(16187..16642)
                     /gene="csgA"
                     /locus_tag="ACHLIF_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO9607507.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16683..17138)
                     /gene="csgB"
                     /locus_tag="ACHLIF_003556"
     CDS             complement(16683..17138)
                     /gene="csgB"
                     /locus_tag="ACHLIF_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO9607508.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            complement(17267..>17523)
                     /locus_tag="ACHLIF_003557"
     CDS             complement(17267..>17523)
                     /locus_tag="ACHLIF_003557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607509.1"
                     /translation="KRSCFFSAPPWTIFYCKKTRFVTACAQDISQQSATGKKNDCLVI
                     FDSGKRRFKRKQNIFCVDNSVFTWVLILWYEQKKKNTTRG"
BASE COUNT         4382 a         4461 c         4150 g         4530 t
ORIGIN      
        1 gcagaaattt atctgttccc ccctttaaaa attcggtgaa tacccttact tattggtata
       61 ctgaagttgc ttcagataaa aacgcattga acctcgaata acgttgtcta gtaacacgaa
      121 ttagggggcc atgatggaaa aaaataatga agtcattcag actcatccgc tcgtagggtg
      181 ggacatcagc accgttgata gctatgatgc gctgatgttg cgtttgcact accagacccc
      241 aaataagtcc gagctggaag ggactgaagt tggtcagacg ctctggttaa ccactgatgt
      301 tgccagacaa tttatttcga tattagaagc aggaatcgcc aaaattgaat ccggtgattt
      361 cccggtaaac gagtatcggc gtcattaaca tgacatgacc atccacccaa aaggcacctc
      421 accggtgcct ttaccatttc tgggttgtat atctgcattc agttaattac tctgcttaaa
      481 ccatgagagc agagagaaaa aaatgaaata cgatctcatc attattggca gcggttccgt
      541 aggcgctgcc gccgggtatt atgcaacccg cgccggttta aacgtgctaa tgaccgacgc
      601 ccatatgcca ccgcatcaac acggcagcca ccatggcgat acgcgattaa ttcgccatgc
      661 ttatggtgaa ggcgaaaagt atgtcccgct ggtcctccgc gcgcaaacgc tgtgggatga
      721 actttcccgc cacaacgaag atgatcccat ttttgtacgc tctggtgtca ttaacctcgg
      781 cccggctgac tccgcatttc tcgccaacgt cgcccacagc gccgaacagt ggcaactcaa
      841 cgttgaaaag ctcgatgcgc aagggattat ggcccgctgg ccagaaatac gcgtcccgga
      901 caactacatc ggcttatttg agactgattc cggttttttg cgcagcgaac tggcgattaa
      961 aacctggatc caactggcga aggaagcggg ctgtgcgcaa ctgttcaact gcccggtcac
     1021 cgtaattcgt catgacgatg atggcgtaac tattgaaacg gttgacggtg agtatcaggc
     1081 gaaaaaagcg attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg agctgcctgt
     1141 ccagcccgta cgtaaagtat ttgcctggta tcaggccgat ggccgctata gcgtgaagaa
     1201 taaattcccg gcgtttaccg gtgaactgcc caatggcgat caatattatg gttttccggc
     1261 agaaaacgac gcgttgaaga ttggcaaaca taacggaggc caggttatcc attcagcgga
     1321 tgaacgtgtt ccgtttgcgg aagtggtcag cgatggttcg gaagccttcc cgttcttgcg
     1381 caatgtattg ccgggtatcg gttgctgcct gtacggcgct gcctgcacct atgataattc
     1441 gcctgacgaa gattttatta tcgataccct acccggccac gataatacac tgctcattac
     1501 cggcctgagt gggcacggtt ttaaatttgc gtcagtttta ggggaaatag ctgccgattt
     1561 tgcgcaagac aaaaaaagcg attttgattt gacgccattc aggctttccc gcttccaata
     1621 atcatcatta cggcctccag aatgaggccg tttttacagg agtatttaat gcgtcggctg
     1681 ttgcactatc tcatcaataa tattcgcgag catctgatgc tttatctttt tctctgggga
     1741 ttgttggcaa ttatggatct gatctatgtg ttttattttt agttttccgc actgacataa
     1801 actgacaaat atttaatctc cctttcattt ggcgtgaaaa attgaatgag attattcctg
     1861 cttctacagc actcaccaga catctctgat gctgaaccca ctaaacttat gaagattctt
     1921 tcattattaa ttcaaaatta ttgaaataaa aataaatatc attcaatttg ttatttttgt
     1981 gttgcaattc attctccgac acgccatttt ctttctctcg caaattcgta ttggatgcac
     2041 ttatgtcatt cacaaatacc cctgaacgct atggtgttat atctgcggcc ttccattggc
     2101 taagcgcaat tattgtctac ggcatgtttg ctttaggtct gtggatggtc acgctcagtt
     2161 attacgatgg ctggtatcac aaagcacccg aactgcataa aagcatcggt attttgttaa
     2221 tgatggggct ggttattcgc gttctgtggc gtgtcatatc tccaccgccg ggtccgctgc
     2281 cgagttattc gcccatgact cgtcttgccg caaaggctgg acatctcgca ttgtacctat
     2341 tgctgttcgc catcggcatc agcggctatc tgatctcaac tgccgatggt aaaccgatca
     2401 gcgtttttgg ctggtttgac gtccccgcga cccttgccga cgctggcgca caggcagact
     2461 ttgccggtgc cctgcatttt tggctcgcgt ggagcgtcgt agtactgtcc gttatgcacg
     2521 gatttatggc cctgaagcat catttcatcg ataaagacga cactctgaag cgcatgctgg
     2581 gaaagtcgtc atctgactat ggagtataaa aaatgaaaaa aagcctgctt ggtttaacct
     2641 tcgcgtccct gatgttctct gccggttcag cggttgccgc cgattacaaa attgacaaag
     2701 aaggtcagca cgcctttgtt aacttccgca tccagcatct tggctatagc tggttatatg
     2761 gcacctttaa agatttcgac ggtactttta cctttgacga aaaaaatccg gctgccgata
     2821 aagtgaatgt gacaattaac accaccagcg tcgatactaa tcacgccgaa cgcgataaac
     2881 atcttcgcag tgcagatttc ctcaataccg caaaatatcc acaggcaaca ttcacctcca
     2941 ccagcgtgaa gaaagacggt gacgaactgg atattaccgg cgatctgacg ctgaatggcg
     3001 taaccaaacc cgtcacgctg gaagcgaaat taattggtca gggcgacgac ccatggggtg
     3061 gtaaacgtgc aggcttcgag gccgaaggca aaattaagct caaggacttc aatatcaaga
     3121 cagatttagg tccagcttct caggaagtgg atctgattat ttcagtggaa ggcgtacagc
     3181 agaagtaagc gtgaaatgtt gccggatggc acgcatccgg caatgatatt tattccgttg
     3241 gatcaggaat gcccagtgtt gtattcagac gtccacgaga cttattaaag atcttattgc
     3301 cattttcacg tcctgcccga cggcgtcgct gttcttctgg cggtaacgcg ctttcttcgc
     3361 agcaaatctc actacaacaa cctttgtatt tttccgcaca tactggacac tgaataaaca
     3421 gcaggtggca gccatcattt ttacagttgg tatggctgtc gcacggcgcg ccgcactggt
     3481 ggcaatgcgc gataatctca tccgatatac gttcgcccat ccgctcgtca aaaacaaaat
     3541 ttttgccaat aaaacgcacc ggcaagccct gctcgcgcgc cttacgggcg tattcaataa
     3601 ttccgccctc gatatgccag actttgttga atccgttatg tttcatccag gcactggcct
     3661 tttcacaacg aatgccgccg gtgcagtaca tgacgatttt tttatcttta tgtgcctgca
     3721 tcatctcgac tgcttttggc agctgctcac ggaaggtatc tgccggaatt tcgagcgcgt
     3781 tttcaaagtg ccccacttca tactcatagt ggttacgcat gtcgataaat agtgcatcgg
     3841 gatcgtcaag catggcgttc acttccgccg cttgcagata ctcgccaaca ttgctggcat
     3901 caaagtgagg atcgtcaata ccgtcggcaa caatgcgatc gcgtaccttc atgcgcagta
     3961 cccagaaaga tttcccgtca tcatccaacg cgatattcag gcgtaaaccc tctaaagccg
     4021 ggtcgaaggc atagagctgc gcgcgaaatg tttcaacatt gctcgcaggt acgctgattt
     4081 gcgcgttaat gccctcatgc gccagataca ctcgcccaaa aacattcagc gcggtaaaca
     4141 gctgatataa agcgtcacgg gtcgccttag gatcggcgat gtggaaatac ttgtaaaacg
     4201 aaatggtggt tcgcggttcg ctctcagcca acattttggc ttttagcgcg tcgttggaaa
     4261 tgcggttgtg taacactggc atggtgtacg gttcctgcga gatgggaaag taaaaatccg
     4321 cggcatgata tagcaattat cgataattaa catccacaca ttttacgcta catttgcgca
     4381 ttaaaaatta tttgttattt acaagtgcgg caatttcgcc cagtcttcag ccacaaattt
     4441 tggttgcggg cgaaaaaatg cgacaataca tacaattgcc cgaataggtt gaaaaacagg
     4501 attgatatga cgaatctacc caagttctcc accgcactgc ttcatccgcg ttattggtta
     4561 acctggttgg gtattggcgt actttggtta gtcgtgcaat tgccctaccc ggttatctac
     4621 cgcctcggtt gtggattagg aaaactggcg ttacgtttta tgaaacgacg cgcaaaaatt
     4681 gtgcatcgca acctggaact gtgcttcccg gaaatgagcg aacaagaacg ccgtaaaatg
     4741 gtggtgaaga atttcgaatc cgttggcatg ggcctgatgg aaaccggcat ggcgtggttc
     4801 tggccggacc gccgaatcgc ccgctggacg gaagtgatcg gcatggaaca cattcgtgac
     4861 gtgcaggcgc aaaaacgcgg catcctgtta gttggcatcc attttctgac actggagctg
     4921 ggtgcgcggc agtttggtat gcaggaaccg gggattggcg tttaccgccc gaacgataat
     4981 ccactgattg actggctaca aacctggggc cgtttgcgct caaataaatc gatgctcgac
     5041 cgcaaagatt taaaaggcat gattaaagcc ctgaaaaaag gcgaagtggt ctggtacgca
     5101 ccggatcatg attacggccc gcgctcaagc gttttcgtcc cgttatttgc cgttgagcag
     5161 gctgcgacca cgaccggaac ctggatgctg gcacggatgt ccggcgcatg tctggtgccc
     5221 ttcgttccac gccgtaagcc agatggcaaa gggtatcaat tgattatgct gccgccagag
     5281 tgttctccgc cactggatga tgccgaaact accgccgcgt ggatgaacaa agtggtcgaa
     5341 aaatgcatca tgatggcacc acagcagtat atgtggttac accgtcgctt taaaacacgc
     5401 ccggaaggcg ttccttcacg ctattaaatc tcccctgccg gatgcttcag aatggcatcc
     5461 ggcattacca cagcaaatcc ccctgattta gcgataaaag ctctctggat tgcgccccct
     5521 ggaagtcgag cgcataatta gtgtgcttat cttttcttct tatgttcacc gcgcctggcg
     5581 caccaacagc ggattgctat gtcaccctgt gaaaatgaca cccctataaa ctggaaacga
     5641 aacctgatcg tcgcctggct aggctgtttt cttaccggtg ccgccttcag tctggtaatg
     5701 cccttcttac ccctctacgt tgagcagctt ggcgttaccg gtcactccgc cctgaatatg
     5761 tggtccggta ttgtcttcag cattacattt ttattttcgg ccatcgcctc accgttttgg
     5821 ggtggactcg ccgaccgtaa aggccgaaaa ctcatgctat tacgctctgc cctcggcatg
     5881 ggcatcgtga tggtgttgat gggactggca caaaatatct ggcagttttt gatcctacgg
     5941 gcgcttcttg ggttacttgg cggatttgtc cccaacgcta atgctcttat cgccacacaa
     6001 gtaccgcgta ataaaagcgg ctgggcgctg ggtacgctct ccacaggcgg cgttagtggt
     6061 gcgttgctcg gcccaatggc tggcggcctg ctcgccgata gctacggctt acgtccggta
     6121 ttctttatta ccgccagtgt gctcatactc tgctttttcg tcaccctgtt ctgcatcaga
     6181 gaaaaattcc agccggtcag caaaaaagag atgctgcaca tgcgggaagt ggtgacatca
     6241 cttaaaaacc cgaaactggt actcagcctg tttgtcacca cgttaatcat ccaggtggcg
     6301 acaggctcaa ttgcccccat tctgacgctg tatgtccgcg aactggcggg taacgtcagt
     6361 aacgtcgcct ttatcagtgg catgatcgcc tcggtgccag gcgtggcggc tctgctaagc
     6421 gcaccacgac tcggcaaact tggcgatcga atcggacccg aaaagatcct gattacagcg
     6481 ctgatctttt ctgtactgct gttgatccca atgtcttacg ttcagacgcc attgcaactt
     6541 gggattttac gttttttgct cggtgccgcc gatggtgcac tactccccgc cgtacagaca
     6601 ctgttggttt acaactcgag caaccagatc gccgggcgta tcttcagcta taaccaatcg
     6661 tttcgtgata ttggcaacgt taccggacca ttgatgggag cagcgatttc agcgaactac
     6721 ggtttcagag cggtatttct cgtcaccgct ggcgtagtgt tattcaacgc agtctattca
     6781 tggaacagtc tacgtcgtcg tcgaataccc caggtatcga actgattttt cgcctttcat
     6841 acttgcaaaa gcggagaatc agctatcctt ttccctgaaa cctcatcaac tcaaagggag
     6901 aatcgtgatg accatgtacg caacgcttga agaagccatt gacgctgcac gcgaagaatt
     6961 tcttgcagac aaccccggca tcgacgccga agatgcgaat gtgcaacagt tcaatgccca
     7021 aaaatacgtt ttgcaggacg gcgacatcat gtggcaagtt gagttttttg ccgacgaagg
     7081 ggaagaaggt gaatgtttac ctatgcttag cggtgaagcc gcgcaaagtg tttttgatgg
     7141 cgactatgat gagatagaga tacgccagga gtggcaggaa gagaatacat tacatgaatg
     7201 ggacgagggg gaatttcagc ttgagccacc gctggatacc gaggaaggac gcgcagcagc
     7261 tgatgagtgg gatgaacgtt aatcactcat acgggccatg atgaatgtcg atcggcagca
     7321 gtaaagtatc aaaaaccaga gagaatggca gatcaaggat cgtgacataa cgccaggcgg
     7381 agtcacgcac atcccattgc acaccgggat aatattggtt gccatgcccc tgccccggta
     7441 tagtgcgact aataatgctg ccacagccac tcagcaaggt caccatgatg ctcaccacaa
     7501 ttaaacgcat ttgatacctc tttcgtaaaa aaataccggc ataacgccgg tatttttatt
     7561 ggctcgttgt ttttgccgga tgcggcgtga acgccttatc cgacccacaa aatcttgcaa
     7621 aatcaataaa ttgcaggaac gatgtaggcc tgataagcgt agcgcatcag gcaactacgt
     7681 ttttattgcg aagccgcatc cggtttacgc aatgccagtg gattgagctt tatcccttcg
     7741 taataactca cccatgaaga atatctctcc ggggaattcc agacgcggaa atgcagacgg
     7801 gccatcgtca ccggatcgct tagcagcacc aggcgacgat cgcgattcag cttctctggc
     7861 gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga tttccagcac cttactggcg
     7921 cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa atgacgggtt aaacactgcg
     7981 tgcataaagc catcatcaag ggagcgttga cgattcatct caaggaaccg atcggtatca
     8041 accagcacct gcggcggcga atactcttcc gggatcagga acagtttcca gcgtttggtg
     8101 cgcagaccaa cggtggcacg gctggaaatc accgaaacaa acggtgacag gatcaacgag
     8161 aagacaatcg gtgccagcca gaacaggaaa cgcagatcca accacgccat accaacagcc
     8221 cacactaacc ctaacagcag ctgtgagccg tggcgtttga acgcttcacc ccaggaagtg
     8281 gagtcatcat cacgctgcgg tgaattccac accacttccc agccaaggaa cgcgctgacg
     8341 acgaagaccg tatggaacag catgcgtacc ggagccagca gcacggaaaa cagcacttcc
     8401 agcagcaacg ataatgtaac gcgccagaag ccgccgtatt ctttcgttcc tttgcaccag
     8461 ataagcaaaa tgctcaataa cttcggcagg aacaacagca ccatggtcga agcaaaaagt
     8521 gcaatcgcca gctcaggacg ccactgcggc cacaccggga acaactgccg tggttgcagg
     8581 aagtattgcg gttcggtcaa cgcatgtact acctgcaatg cagtagagag cgcgaggaac
     8641 ataaaccaca gcggagcgga gagataagac atcacgcccg tcaggaatac cgcacggtga
     8701 accgggtgca tacccttcac caggaacaga cggaagttca tcaggttacc gtggcaccag
     8761 cggcggtcac gttttagctc atcaagcaag ttaggcggca attcttcata agaacccggg
     8821 agatcgtaag caatccagac cccccaacct gcacggcgca tcaacgccgc ttccacgaag
     8881 tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg gcagcggagc cagtgcgcag
     8941 tgctcgataa acggtttcac gcggataatc gcgttatgtc cccagtagtg cgactcgcca
     9001 agttgccaga agtgcaaacc ggctgtaaac agtggcccat acacgcgggt cgcgaactgc
     9061 tgacagcgcg catacagcgt atccataccg gacgctttcg gcgacgactg aatgatcccg
     9121 gcgttcgggt tggcttccat caggcgcacc aggccgcata aacaatcacc ggtcattacc
     9181 gagtcagcat ccagcaccac catgtagctg tactggctgc cccagcgacg gcagaagtca
     9241 tcgatattac cgcttttacg cttcacgcgg cgacggcggc ggcgatagaa aatctgacct
     9301 tcgccaccga cttcagcgat aagctccatc caggcttttt gctctgcgac gcagatatcc
     9361 gggttataac tgtcactaag aatgtagaca tcaaagtgtt tggcattccc ggtggctttt
     9421 actgattccc acgttgcacg caggccagca aaaacacggt tcacgtcttc gttacagata
     9481 ggcatgatca acgccgtgcg atgctccggg tttaatggtt catcgccaac tgttgacgca
     9541 gatatactgt atttatcgcg accaataagc agttgcagga agcccattaa cgccgtccag
     9601 aatccggcgg acacccaaca gaacagtacc gcaaagagga tcaggatacc ggtttgcagc
     9661 atataaggca gaagctgcat aaaggaaacc cacaaatcct gaccaaccat atccatagga
     9721 ttaatcagcg cccaaccctg ataaggaaga atggtcttca tataccaggt cgcgacgaca
     9781 gtttgcgcga gcgtcaggat caacagaatg taacggcgga tggtaccgac ggtacgccac
     9841 ttttgctcgc tctcctgctc ttctttggtc aaacgagcca gatagcgcgg cgtgacatcg
     9901 cgtccacgca ggcgatccca gaaacggcct accgggttgg tacgccacgg gtcgggaaac
     9961 atcgaggagc gttttgcttc tggcatcgcc ttcagctgat cgcgcccttc gtcgtcttta
    10021 attaactgtc catcagcaag tgaatctggc caggcttgtt ccagacgcgc ctttaccgag
    10081 ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt cggcatccag cgcctgatga
    10141 acggcgcgga tatcagtctt cggcaatgcc gctttctcgc ttgcggcgat gggcattgcg
    10201 tcaatgtact cagttgtctt attcattggc aggtaactgg tagctccagg tttcactcaa
    10261 cgtctgatcg gcattcacca gcgcagcacg catttcagtg gttttcttgg catctttcac
    10321 tttcacacgc atcaccagac gccagccttt ggtaaccggg ttatagcgca ccgtgctttc
    10381 aactatctca ccattatcac caatgctggt ttgcgctgtg accggggtat cctctggcag
    10441 ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg atagtaccgt caggctggcg
    10501 aatcaggttc gactgcttca catcccccgt tgaacgacgc gtttgttgca cccatgcgtt
    10561 atctggcgca tgcagtttgt cttcatcacg gctgaaggtg atggtgtatt taaagttcat
    10621 ctctttaccc ggctccggca gctgatccgg cgtccagtaa gcgacgatgt tatcgttggt
    10681 ttcatcgttg gttggaattt ccaccagctc aacgctgcct ttaccccact cccctttcgg
    10741 agtcacccat gcgcttggac gaagatcgta acgatcatcg agatcttcaa agcgggagaa
    10801 atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttc tccatcgaga agctgctgac
    10861 cgctaaatgt ttcgggttat tcaacggacg ccagatccat tcgccattac cggcatggat
    10921 agacagaccg ttagagtcgt gcaactccgg acgatagtta tttgcaggcg acggttggtt
    10981 cggcccaaac aggaacatac tggttaacgg tgcaaccccc agtttgccga ctttatcgcg
    11041 cagatagatt ttcgactgca catccacaac cgtgtcacgc cctggcataa ccacgaattt
    11101 gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa atggttaaac gtttatcagt
    11161 cggttttgga cgctcgatcc agaactcttt gaagcgtgga aattcttcac ccgatggcaa
    11221 ggcggtatca attgccaggc cgcgggcaga aaggccataa acctgacctg caccaatcac
    11281 gcggaaatag ctggccccga gcatgctgac gatttcatcg tttttatctt tgctgttgat
    11341 cgggtaaagc actttaaaac cggcaaaacc aaggtctttt accgtgtctt tgtcatgctg
    11401 aacatcgccg aaagtgaaat aatccgggct gtatttgatt cgtttgactg cggtggcagt
    11461 cacttcattt attttgaccg gggtatcgaa gtacataccc tgatggtaga actcgagttt
    11521 gaatggggtc ttcagattgt tccagtacgc tttgtcatga ttaaactgga tctgctgata
    11581 gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg cttttgggcg cctcatagcc
    11641 tttcccggct aaggattgag cttgctttgc gacatcatca atactgaaag cccagcttga
    11701 agatgtatac agggttaaca ttactgcagc actcaaccaa cgcattttca tcatttgtag
    11761 tttatgtttc ataattagta agcacttccc cctttgtgtg cttatatcga tccgatctat
    11821 tttaatggat aaccagccat tccgacaaca gaatcccggt atcgttcagc acgctggctc
    11881 atggattaag cacctgattg caaccgtttt cactttccgt gcttctccta aagacagaag
    11941 catcgacttc gtgtagggtt tgccagcaat tttatttatc aaccgtctta cagacgcttc
    12001 cattctggtt agtggtagcg atgatgctat gcataaaaac cttgaagcct gcgcctggcg
    12061 cggtcaggtc ggtgtcgcta tggcaacaag cctaaaggca taacccgaca gggataagac
    12121 gaaaaatccg agattagtta accatatatg aatccagtac ccgcgcaacg tgaatatttc
    12181 ctcgactcca tccgcgcctg gctgatgttg ttagggatcc cttttcatat ttctttaatc
    12241 tattcgagcc atacatggca tgtgaatagc gccgaaccat cattgtggct gacccttttt
    12301 aatgacttca tccactcgtt ccgcatgcag gtatttttcg ttatatccgg ctacttttcc
    12361 tacatgcttt ttttacgcta tcccttgaaa aaatggtgga aagtacgtgt cgaacgtgta
    12421 ggtatcccga tgttaacagc catcccccta ctgacattac cgcaatttat tatgctgcaa
    12481 tatgtcaaag gaaaagcgga aagttggcct gggctgtcat tgtatgacaa atataatacg
    12541 ttggcctggg aattaatatc acacctgtgg tttttactgg tgttagtagt catgacgacg
    12601 ctgtgcgtat ggatatttaa gcgcatcaga aataatttag aaaattctga taaaacgaat
    12661 aaaaaattct cgatggtaaa actatcggtg atttttttat gcctcggcat cggttatgcg
    12721 gtaataagaa gaacgatttt tattgtgtat ccgcccattc tgagtaatgg catgttcaat
    12781 tttattgtca tgcaaacgct gttttatttg ccgttcttta tcctcggcgc actggctttc
    12841 attttccctc atcttaaagc cttgtttacc acgccgtctc gtggctgtac ccttgcagca
    12901 gcattggcgt ttgtcgctta tttactcaac cagcgctatg gcagtggcga tgcctggatg
    12961 tacgaaaccg agtcggtgat caccatggtc ctcggtctgt ggatggtgaa tgtggtcttc
    13021 tcctttggcc accgtttgct taacttccag tcagcgcggg tgacttattt tgttaacgca
    13081 tcgctgttta tctatctggt tcaccacccg ttaacgctgt ttttcggcgc atacattaca
    13141 ccgcacatca cctccaactg gcttggtttt ctctgtggcc tgatatttgt agtagggatt
    13201 gcgataattc tgtatgaaat tcatttgcgc atcccgttac tgaagttttt gttctctggt
    13261 aaaccggttg ttaagcgtga gaacgataaa gcaccagccc gttaagccac atttacaata
    13321 accattccac gggcaatatc gacgccagtc tgaccataac ccgcttccag aaactggtgg
    13381 cgggttcttt tttgagaata atctctttct ttgcatgacg atcaacccag ttgatccgtc
    13441 cccacctgtc cagacggagc tgccaggccg catcatactg gctctgaata aagcgtttat
    13501 caattaactg tgccagcgtt tcgctctcta tcacgaagcc catttcagta ttgagcaatg
    13561 ttgaacgcgg atcgaaattg aaagagccga taaacaccgt tttaccatcg atgctaaagg
    13621 ttttagcatg caggctggct ccggaattac cggttatgcc gcgatcgtgt aacgtactac
    13681 tttgttcacg cgtcggcttg agttcatata attccacgcc atagcggagc aattttttgc
    13741 gccagcgcgc gtatccggca tggacgacag caacatcgtt agcggcaaga gaattggtta
    13801 ggatcgcaat ctttacccct tttctcacca tccgtaagag ttgcgccaca cctgcgcgtg
    13861 tcggtacaaa ataggaagag ataatatcga tgcgttcact gggtgagccc atgatatcga
    13921 acaggcgctg cggtaacagt gaatgccgtt ttgccttgcc ctcccctttc gccggatcat
    13981 cacttaataa acgtgttttc gcccagataa gcggcaacgt tccatcaacc agatgattta
    14041 taaacggact ggattccatt ttgcgtaagt aacgatgcgt catagcatcg ttatgccagg
    14101 aggcgggtaa ctcgatgcga tccgccattt caccttccgg gacatccagc acttgctgta
    14161 agggtgaaac cgatttgcaa taccagtagc gggcgaaatc atcggcaacg tcctctacca
    14221 cgggccctat tgccataaca tctaaatccg aaaaaagtgg ctcttcccct gccccaaaat
    14281 aggcatcacc aatatttcgc ccccctacca gcgtcaccac gccatcgaca gtgaaacttt
    14341 tattgtgcat acggcgattg agacgggaaa agtcggtgat ataaccaagc ggacgcagca
    14401 agcgaaacga gaaaggatta aaaagccgga cttcaatgcg gggatggctg tcaagcaagc
    14461 gtaaaatgtc gtcaagtccg ggcgtattgt tgtcgtccag caacaaacgg acgcgaacgc
    14521 cacgctttgc tgcggctaac agggcggaaa acagtaaccg acccgacata tcgtcctgcc
    14581 agatgtaata ctgaacatcc agcgtatgct ctgccatttc ggccagacga taccgggcgg
    14641 caaacgcatc cagacttttc tccagaggga aaagaccaca ctgaccggga tgttgcgaac
    14701 acagtggcag caccgcgctc gccagccggg gcaaatcatt catctccttg ttgggtaagg
    14761 agtctttcgt agaggtgggc gttttcttca tcataacaga caaagtatac ctgttcgggt
    14821 aaagcgtgac gggtaataaa ttctgaaacg gttttaactg cgatttcagc cgctgccgca
    14881 cgagggtaac cataaacccc agtactgatt gcaggaaaag ccactgacgt atagctgttt
    14941 gccgccacca gtcgtaggct attgagatag gcatcctgca aaagctggtc ttcgttttgt
    15001 tcaccaccac gccagactgg cccgacggtg tgcactacgg ctttagcggg aagagcgcct
    15061 gcaagcgtaa taacggcatg ccccgtaggg caatcgccct gctgttgcct gacttttaaa
    15121 caggcatcca gcagggccgg acccgctgcg cgatgaatgg ccccatcgac gccgccgcct
    15181 cccattaatg acggattagc cgcattcaca atcacatcaa cggccagttt ggtaatatca
    15241 ccctgcacaa catgaatacg cgttttcata acgcctcctt acaccttctt gtttcttaag
    15301 tgtacagcag gctttttggt taaatcgcat ttttctgctc agcaacgtgc gttgttcaga
    15361 aacttagtta tattcgatgt tcattacagc cagctttttc tgtttatcaa gatactgcac
    15421 ttttactgag gctatttgcg cgtttttaac attaccgctc attaacgctt tgctggaata
    15481 ggttttgccc ggaacagaac cattctgtgg gcagttcatt tctacgggtg atgactgggt
    15541 gctgacgtca catgccggtt ccacaatttg gccataaaaa tggatcaccc ccccttgcgc
    15601 ggtgcttcct gcgatggcaa taaaaggaaa aagcaaactg ccgagcataa gagagtttaa
    15661 aaaaggtctg aacatttatt ctatttcctc aatgattagt catccttgag ggttgtgtta
    15721 tccatacttt caggttggct gattcgcagc agaccattct ctccagattc atcttatgct
    15781 cgatatttca acaaattaag acttttctga agagggcggc cattgttgtg ataaatgaag
    15841 tgactgtcca tcagaaacag taacaactat tttcacccga tcttccgggg aaatatttaa
    15901 actcaacttc gtcaaagcaa taggttgatt agcaggcaat gagagggtct tttcttgctt
    15961 cgtctgactt tgccctgaac tgccttcgcg cagggacaat atttgtactc tgcacagaca
    16021 agattgagta agagtgactt caggaataat ggtatacaca tccccttgct gggtcgtatt
    16081 aaaggttatc tggctggaaa gtgccgcaag gagtaataac gcattcatat tcttctcccg
    16141 aaaaaaaaca gggcttgcgc cctgtttctg taatacaaat gatgtattag tactgatgag
    16201 cggtcgcgtt gttaccaaag ccaacctgag tcacgttgac ggaggagtta gatgcagtct
    16261 ggtcaactgc agcaccgttg ccaccaccga actgtttaac agtcatttca gaattttttc
    16321 cgttccactg atcaagagtg gcgctgttac cgaagccacg ttgggtcaga tcgattgagc
    16381 tgtcatctga gccctgacct acatctgcac cattaccacc gccatgctgg gtaatagtca
    16441 agtcagagtt acgggcatca gtttgcagag caagtgcaga gttaccgcca ccgtactggt
    16501 aaatgttcag ctctgaattt gggccgctat tattaccgcc accaccgtgg ttaccgccgc
    16561 cgccgtactg aggaacaaca cctgccagag cgctaccgga gaatacgatt gctgcaattg
    16621 ctgctacttt taaaagtttc atgtaaaacc cccatcggat tgatttaaaa gtcgaatgga
    16681 aattaacgtt gtgtcacgcg aatagccatt tgcgactgtc tctgcactac aattgccgtt
    16741 ttttgagtac catactgtgt aatatttgct ttattaccag aacctttctg gataatcatc
    16801 gcagtattac cataagcacc ttgcgaaata ctggcatcgt tggcactgcc cgcctgatca
    16861 atatatgcaa ggttataatc tcctgtctgg tcaatctttg cccggttgct actaccttct
    16921 tgcgcaacaa ccgccaaaag ttttgagcct ccctgccgta actgagcact attattcgtc
    16981 ccagcttgac caattatggc tgcctgatta aatgaagact tactcaattc atttaccgcg
    17041 aagttatatt ctgaattagc taaatcataa cctgctgcgg ctgcaatccc aggcgcaccc
    17101 agtattgtta acatcataaa taacaatttg tttttcatgt tgtcaccctg gacctggtcg
    17161 tacatttaag aaattaaatc atttcaactt ggttgttaac gcaacctgta ttttgttaac
    17221 gctgcgttac gatggaaagt atgtctgcgg aaatattttt aataactcac ccgcgcgttg
    17281 tatttttctt tttttgttca taccaaagta ttaaaaccca cgtaaatacg ctgttatcta
    17341 cgcaaaaaat attttgtttt cttttaaatc tccgttttcc gctatcaaaa atcaccagac
    17401 agtcattttt cttgcccgtc gctgattgct gcgatatgtc ttgcgcacaa gccgtgacaa
    17461 acctcgtctt tttgcagtaa aaaattgtcc acggaggtgc ggagaaaaaa caagaacgtt
    17521 tta
//
LOCUS       DAZGOB010000099        17460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGOB010000099 DAZGOB010000000
VERSION     DAZGOB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.099"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(101..832)
                     /gene="rlmB"
                     /locus_tag="ACHLIF_003558"
     CDS             complement(101..832)
                     /gene="rlmB"
                     /locus_tag="ACHLIF_003558"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO9607510.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1012..3453)
                     /gene="rnr"
                     /locus_tag="ACHLIF_003559"
     CDS             complement(1012..3453)
                     /gene="rnr"
                     /locus_tag="ACHLIF_003559"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO9607511.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRSGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3492..3917)
                     /gene="nsrR"
                     /locus_tag="ACHLIF_003560"
     CDS             complement(3492..3917)
                     /gene="nsrR"
                     /locus_tag="ACHLIF_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO9607512.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4122..5420)
                     /gene="purA"
                     /locus_tag="ACHLIF_003561"
     CDS             complement(4122..5420)
                     /gene="purA"
                     /locus_tag="ACHLIF_003561"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO9607513.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5524..5721)
                     /gene="yjeT"
                     /locus_tag="ACHLIF_003562"
     CDS             complement(5524..5721)
                     /gene="yjeT"
                     /locus_tag="ACHLIF_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO9607514.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5803..6807)
                     /gene="hflC"
                     /locus_tag="ACHLIF_003563"
     CDS             complement(5803..6807)
                     /gene="hflC"
                     /locus_tag="ACHLIF_003563"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO9607515.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6810..8069)
                     /gene="hflK"
                     /locus_tag="ACHLIF_003564"
     CDS             complement(6810..8069)
                     /gene="hflK"
                     /locus_tag="ACHLIF_003564"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO9607516.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8155..9435)
                     /gene="hflX"
                     /locus_tag="ACHLIF_003565"
     CDS             complement(8155..9435)
                     /gene="hflX"
                     /locus_tag="ACHLIF_003565"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO9607517.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTGRLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9512..9820)
                     /gene="hfq"
                     /locus_tag="ACHLIF_003566"
     CDS             complement(9512..9820)
                     /gene="hfq"
                     /locus_tag="ACHLIF_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO9607518.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9906..10856)
                     /gene="miaA"
                     /locus_tag="ACHLIF_003567"
     CDS             complement(9906..10856)
                     /gene="miaA"
                     /locus_tag="ACHLIF_003567"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO9607519.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAVEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10849..12696)
                     /gene="mutL"
                     /locus_tag="ACHLIF_003568"
     CDS             complement(10849..12696)
                     /gene="mutL"
                     /locus_tag="ACHLIF_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO9607520.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVYAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12706..14043)
                     /gene="amiB"
                     /locus_tag="ACHLIF_003569"
     CDS             complement(12706..14043)
                     /gene="amiB"
                     /locus_tag="ACHLIF_003569"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO9607521.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14062..14523)
                     /gene="tsaE"
                     /locus_tag="ACHLIF_003570"
     CDS             complement(14062..14523)
                     /gene="tsaE"
                     /locus_tag="ACHLIF_003570"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO9607522.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14495..16042)
                     /gene="nnr"
                     /locus_tag="ACHLIF_003571"
     CDS             complement(14495..16042)
                     /gene="nnr"
                     /locus_tag="ACHLIF_003571"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO9607523.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16041..17180
                     /gene="queG"
                     /locus_tag="ACHLIF_003572"
     CDS             16041..17180
                     /gene="queG"
                     /locus_tag="ACHLIF_003572"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO9607524.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAAKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17163..17216)
                     /locus_tag="ACHLIF_003573"
     CDS             complement(17163..17216)
                     /locus_tag="ACHLIF_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607525.1"
                     /translation="MRFYFYSQAVDESGVTW"
BASE COUNT         3967 a         4979 c         4378 g         4136 t
ORIGIN      
        1 aatattaata aaatgaatat ttgcagtacg taattatctt accagctata gacaaaaaaa
       61 aaccatccaa atctggatgg cttttcataa ttcagacgaa ctagctgcgc tgacgaaccg
      121 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      181 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      241 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      301 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      361 caaggttagt cacccgaatc agtggaacgc tttctgccgc gccgcaggct acttttttcg
      421 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcgtga acgccagcag
      481 cgtccgcgct acgcaggcac gcgccgaggt tgtgcggatc ggttacaccg tcgaggatca
      541 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      601 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      661 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      721 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      781 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      841 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      901 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      961 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
     1021 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1081 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1141 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac gacgcttgcc
     1201 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1261 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1321 ttcgactcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1381 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1441 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1501 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1561 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1621 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1681 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1741 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1801 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1861 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1921 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1981 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     2041 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2101 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2161 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2221 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2281 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2341 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2401 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2461 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2521 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2581 gccgctgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2641 aatggtgacc agcggtaaat cgcgcagatc aacacggccc gcttttgctt cttccggcac
     2701 ttcttctttc agcccggcaa cctgttgctc aacagcctgc ggccagatgt acggaatttc
     2761 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2821 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2881 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2941 acggctatcg tcaggaacca caaagccgac gcccgcttca gtaaagtagc gaccaacaat
     3001 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3061 agcgcccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3121 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3181 gccaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3241 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3301 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3361 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3421 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3481 gaagattttc gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3541 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3601 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3661 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3721 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3781 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3841 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3901 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3961 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     4021 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4081 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacggatcg
     4141 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacacca
     4201 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcagacc gctacgatct
     4261 ttcacgccga aggtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4321 ttccagtcgt cagctgccag cggagtggtc gtcacttcac gaccatccgg catacggtaa
     4381 gccacacaga gtttcacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4441 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4501 cgacgacgac gccccgtagt tgcgccgaat tcgttaccct gcttgcagag gaactcgcca
     4561 gtttcatcaa acagttcggt cgggaacgga cctgcaccta cacgagttga gtaagctttg
     4621 aggataccca gaacataatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4681 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4741 gtaccctgcg caccttcaaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4801 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4861 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4921 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4981 agaccgcgac gtgctacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     5041 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg
     5101 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5161 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5221 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5281 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5341 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5401 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5461 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt
     5521 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5581 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5641 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5701 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5761 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5821 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5881 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5941 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcatgat
     6001 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6061 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6121 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6181 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6241 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6301 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6361 gagggtcaga cgaccacggg aatcagtgac gatatctttc acgtccaggc gaccaatttc
     6421 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6481 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagaatc
     6541 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6601 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagacccgg
     6661 ctcataaacc agaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6721 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6781 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6841 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6901 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6961 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     7021 aggtttccac ctttgtcgtt aaccagcact ttgcgggtgt tgcccaacac tttttccatc
     7081 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7141 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7201 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac
     7261 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7321 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7381 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7441 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7501 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7561 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7621 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7681 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7741 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7801 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7861 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7921 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7981 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     8041 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8101 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8161 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8221 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8281 ctgataaaaa cgacttctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8341 cacctcgccg gaaagccgcc ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8401 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8461 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8521 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8581 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8641 cgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8701 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8761 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8821 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8881 acgctgcttt tcaactcttt ccaggcgtga ctgtatctgc acgatgcgat tacgcaacaa
     8941 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     9001 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9061 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9121 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9181 gctcagggca tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat
     9241 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9301 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9361 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9421 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9481 gctccccgtg taaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg
     9541 cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt
     9601 tgttactgtg atgagaaacc gggcgagacg ggacaacagt agaaatcgcg tgcttgtaaa
     9661 ccatctggct gaccgtgttt ttcaacagga tcacgaattg atcaaaagac tcgatttgcc
     9721 cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg
     9781 cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc
     9841 ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta
     9901 cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcgcgcgc ctgttctggt
     9961 ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc
    10021 gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct
    10081 tcaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc
    10141 aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga
    10201 tgaaaacgct gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac
    10261 tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta
    10321 cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc
    10381 cttgctgccg caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc
    10441 tctaccgcct gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac
    10501 aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc
    10561 cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag
    10621 taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca
    10681 gcgttcggct tcgccgtccc gatatccatc cctttgtaaa taagggcaga atcaacgctt
    10741 atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc
    10801 gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt
    10861 cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt
    10921 cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga
    10981 ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc
    11041 gaatacggac tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg
    11101 ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc
    11161 aatacccaat tccgccaggg cagactgcgc tttttctaat gccgattttt cttcgccaga
    11221 aacttttagc cgcaacggaa tcagcaacgg ctgggcgtaa actggcgctt cacccggcgt
    11281 cagttgcgcc tgacgcagcc aacgttctgc cactggcaag gctaaaagtg aaatgttgcc
    11341 atcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg
    11401 actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc
    11461 gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc
    11521 attcggccag ggggcagccg gacgactgcc tgaggcgggc gcaggagagt agcgcggagc
    11581 taccggctca cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc
    11641 cggaatggga cgcggtgcag gttggggttc atcgtccagc ggtagcggcg tttccagttg
    11701 ctgttgtagc acgctcagca cgccctgata gataaaatca tgcaccagac gcgactgatg
    11761 gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc
    11821 cagatacaac acaaacgccg gttgttgatc ggcccccagt ttgtcttcgc aggcctggcg
    11881 gatcgcatga ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat
    11941 ttccgccagt gcgggcgtag tgtgatttgg atcggccacc cagccgcgca gcgtgagatc
    12001 gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccac aaatcgcgcc
    12061 taagcgccgt tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc
    12121 gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc
    12181 atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt
    12241 gtagaacaga tccagcacct ccagcgtcgt ccccacagga tgcgccgccg gttttaccgt
    12301 cacgttcata tcgcgccctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg
    12361 tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc
    12421 caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc
    12481 cagcgccagc gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag
    12541 tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt
    12601 ttccaccagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg
    12661 gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt
    12721 gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gccccctgcg gcgcagattg
    12781 catcggatgc gcaaggaaat aattgcgcag gcctttgtaa atggcttctg ccagctgttg
    12841 ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc
    12901 gaccagtact gacgggatat ccggcgaacg cagaacgccg agactcgcgt gttctggtcg
    12961 gcgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata
    13021 ccctacccgt tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc
    13081 agactgactg ttcgccagca catcacccgc cccacccagc agctccgact gtttctcgtg
    13141 ctgctccagc cagctggcca tttcgctgtt ggcgcgacgg ttagagagca cccatacgga
    13201 agcgccagtc gcactgcggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc
    13261 gttttgctta cgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg
    13321 ggttaaaacg cccttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat
    13381 ggcgatggtg acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc
    13441 gccgtgtccg gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc
    13501 tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt
    13561 aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg
    13621 tttcgcaacc acgggcggcg gaggaggagg tggcggcgca tcggcgttaa tcgtaaagac
    13681 gacagtgtaa ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggtaag
    13741 atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac
    13801 cagattattg ccgctgaaca acaacggcag tccctgaatc acgcccgttt gtttgatatc
    13861 gagcgccacg gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact
    13921 caacgttatc cgcgcctgtt gattaccgtt agaaacctga atatcagaga gcgtcgcggc
    13981 acccaccggc gtgcacagca gcagcagcgt cgctaccaac caatttctga tgcgatacat
    14041 catcccgcca cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga
    14101 ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac
    14161 atccgggtca ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc
    14221 gttggcaaaa taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta
    14281 caaatcaaag tgataaacca ttaaattgtc gagcgtatag ggttcgacca gcgtataagt
    14341 ggggcttttg acattaccct gatgacccag agcttgtaaa aagccccggc taaaggtggt
    14401 tttacctgcg cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc
    14461 tacccgctcg cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat
    14521 catggttttt atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg
    14581 ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgca gcaccgtgcg
    14641 cgacacagcc cgcacaggcg gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa
    14701 taataccaga gagcacatcg cccatgccgc cgctcgccat gcctgcattt ccggcatcaa
    14761 taatgcctaa agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta
    14821 cgccgccata acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag
    14881 cgacggaaca gcctaacaac cgtgcggcct cgccaggatg cggcgtgatc acgcgattgt
    14941 gacgcttatc gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt
    15001 tgcgaaaatt ctcaactttt tgcagtgctt ttttccccca ctcttgctgg cccagaccgg
    15061 gaccaatcac caccacatcg gcccattcca ggctttcggt aagagagtcc atcgtcagtt
    15121 catgcaccat caattccggt cgtgcagtca gcagcggcgc aatgttttca ctgcgggtca
    15181 gtactcggac taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc
    15241 ccgccgtgcc gtgatcgcca ccgataatta ccagccgccc gtgatcgcct ttatgcgaag
    15301 tcgggcgacg cggttttagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg
    15361 tctcctgacc tgccagccaa ctatccagcc ccagtgagtc aaaatgcagt tgtccggtaa
    15421 catcccgcgc ttttccagtg agcaagcctg gtttcagcgc aataaaagtg atggtgtgat
    15481 cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat
    15541 caaccgccgc aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt
    15601 cgcggggcgc ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga tctaccgatt
    15661 cgggccagac aatattcgaa gcatggatct cgcctcccgc gtttaaccat gcttcgcgtg
    15721 ccagcgcggc ctcttccggc aacggtttgt cgctctcctg ggccaacaac gtgacctcaa
    15781 tgccgaccgc tgtggccagt cgcgcgacca cgtagccatc gccgccgtta ttaccatgac
    15841 cgcacagcac cagccagtgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg
    15901 cttcgccagc gcgaagcatc agctcataga gtgtaagccc cagcgcatct gccgcctcac
    15961 gttctccgcg gcggatatcg tcggcgtgcc agacggtgtg tggtatactt acggggtttt
    16021 tcttcattgt atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa
    16081 ttaaacagtg ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca
    16141 gcgagtccga gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact
    16201 ggatggcacg tcacggtatg ctgcgcgctc gcccccatga gttattaccc ggtacgctgc
    16261 gcgtgatcag cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga
    16321 aaaaccccaa actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc
    16381 tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt
    16441 ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc gttagctgca aaagctgggc
    16501 tcggctggac aggtaagcac tcacttatcc tcaatcgcga ggccggttct ttcttctttt
    16561 taggcgaatt gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg
    16621 gtaaatgcat agcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg
    16681 tcgatgctcg ccgctgtatc tcttatctca ccatcgaact ggaaggggcg atcccggaag
    16741 agttgcgacc gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc
    16801 cgtggaatcg ctattcgcaa ctcactacag aagacgattt cagcccgcgt aagccgctac
    16861 acgcaccgga actcattgag ttattcgcct ggagcgaaga gaagttttta aaagtcacgg
    16921 aaggatcggc gattcgccgt ataggtcacc tgcgctggct gcgtaatatc gccgtggcat
    16981 taggcaatgc cccctgggat gaaacgattc tggcggcgct ggagagtcgc aaaggtgagc
    17041 acccacttct tgatgagcac atagcgtggg cgatggcgca gcaaatcgag aggcgaaatg
    17101 cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag
    17161 ggttaccacg tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca
    17221 attcaagacg aataaaatcg tcaagtgatc gactcgacaa aacgaaatct taatttttaa
    17281 cttatctatc aataagttaa aaaaatacta ttcaccgtgc gaagtttctt tgaaattaag
    17341 actacagcct gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacaaaaac
    17401 cagcaataac gcatcgcgtc gctgtggata ttttattgag agaagaattt ggagcgggaa
//
LOCUS       DAZGOB010000100        17432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGOB010000100 DAZGOB010000000
VERSION     DAZGOB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.100"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(27..326)
                     /gene="ihfA"
                     /locus_tag="ACHLIF_003574"
     CDS             complement(27..326)
                     /gene="ihfA"
                     /locus_tag="ACHLIF_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO9607526.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(331..2718)
                     /gene="pheT"
                     /locus_tag="ACHLIF_003575"
     CDS             complement(331..2718)
                     /gene="pheT"
                     /locus_tag="ACHLIF_003575"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO9607527.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(2733..3716)
                     /gene="pheS"
                     /locus_tag="ACHLIF_003576"
     CDS             complement(2733..3716)
                     /gene="pheS"
                     /locus_tag="ACHLIF_003576"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO9607528.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(3926..4050)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(4000..4044)
                     /gene="pheM"
                     /locus_tag="ACHLIF_003577"
     CDS             complement(4000..4044)
                     /gene="pheM"
                     /locus_tag="ACHLIF_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO9607529.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(4167..4523)
                     /gene="rplT"
                     /locus_tag="ACHLIF_003578"
     CDS             complement(4167..4523)
                     /gene="rplT"
                     /locus_tag="ACHLIF_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO9607530.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(4576..4773)
                     /gene="rpmI"
                     /locus_tag="ACHLIF_003579"
     CDS             complement(4576..4773)
                     /gene="rpmI"
                     /locus_tag="ACHLIF_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO9607531.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(4870..5412)
                     /gene="infC"
                     /locus_tag="ACHLIF_003580"
     CDS             complement(4870..5412)
                     /gene="infC"
                     /locus_tag="ACHLIF_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO9607532.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(5416..7344)
                     /gene="thrS"
                     /locus_tag="ACHLIF_003581"
     CDS             complement(5416..7344)
                     /gene="thrS"
                     /locus_tag="ACHLIF_003581"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO9607533.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            7868..9767
                     /gene="espL1"
                     /locus_tag="ACHLIF_003582"
                     /pseudo
     CDS             7868..9767
                     /gene="espL1"
                     /locus_tag="ACHLIF_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            9939..10046
                     /gene="yniD"
                     /locus_tag="ACHLIF_003583"
     CDS             9939..10046
                     /gene="yniD"
                     /locus_tag="ACHLIF_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO9607534.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(10099..10857)
                     /gene="ydiY"
                     /locus_tag="ACHLIF_003584"
     CDS             complement(10099..10857)
                     /gene="ydiY"
                     /locus_tag="ACHLIF_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO9607535.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            11144..12073
                     /gene="pfkB"
                     /locus_tag="ACHLIF_003585"
     CDS             11144..12073
                     /gene="pfkB"
                     /locus_tag="ACHLIF_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO9607536.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12174..12464
                     /gene="ghoS"
                     /locus_tag="ACHLIF_003586"
     CDS             12174..12464
                     /gene="ghoS"
                     /locus_tag="ACHLIF_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO9607537.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            12570..13430
                     /gene="yniA"
                     /locus_tag="ACHLIF_003587"
     CDS             12570..13430
                     /gene="yniA"
                     /locus_tag="ACHLIF_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO9607538.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(13471..14007)
                     /gene="yniB"
                     /locus_tag="ACHLIF_003588"
     CDS             complement(13471..14007)
                     /gene="yniB"
                     /locus_tag="ACHLIF_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO9607539.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            14154..14822
                     /gene="hxpB"
                     /locus_tag="ACHLIF_003589"
     CDS             14154..14822
                     /gene="hxpB"
                     /locus_tag="ACHLIF_003589"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO9607540.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            14985..15575
                     /gene="ydjM"
                     /locus_tag="ACHLIF_003590"
     CDS             14985..15575
                     /gene="ydjM"
                     /locus_tag="ACHLIF_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9607541.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            15708..17099
                     /gene="tcyP"
                     /locus_tag="ACHLIF_003591"
     CDS             15708..17099
                     /gene="tcyP"
                     /locus_tag="ACHLIF_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO9607542.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(17103..>17432)
                     /gene="ydjO"
                     /locus_tag="ACHLIF_003592"
     CDS             complement(17103..>17432)
                     /gene="ydjO"
                     /locus_tag="ACHLIF_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFO9607543.1"
                     /translation="KKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDS
                     SLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPI
                     QNGPLPA"
BASE COUNT         4431 a         4369 c         4056 g         4576 t
ORIGIN      
        1 agagcggcct ttttagttag atcagattac tcgtctttgg gcgaagcgtt ttcgacccgg
       61 ctttttaact tctgcccggg tctgaaggtc accacgcgcc gtgctgtaat gggaatatcc
      121 tcgcccgttt tcgggttacg tcccgggcgt tgattcttat cacgcagatc gaagttacca
      181 aaaccagaga gtttcacctg ttcgccgttt tccagagcgc gacggatctc ttcgaaaaac
      241 agttcaacca gttctttggc atcccgcttg ctaagcccaa gcttatcaaa cagatattct
      301 gacatttcag cttttgtaag cgccataggt tcaatccctc aatgatgcct ggaatcgctc
      361 ttttaatgcc tctacacatt tggcgacggt agcggcaatc tcctcttctt cgagtgtacg
      421 gctggtatct tgcaggatca ggcttatggc gaggctctta tacccctccg caacaccctt
      481 accgcggtac acgtcaaata agtttacgcc aactacctga tttacgccaa ctttcttaca
      541 ttcggataaa atatccgctg cgggaacgtt ttctgcgacc accaccgcga tgtcacgacg
      601 gttcgccggg aagcgagaaa tctcgcgcgc ctgaggcacc acgcggtctg cgagcttgtt
      661 ccactccagt tcgaacacca gagtgcgacc gttaagatcc agtttacgtt ccagttcagg
      721 atgaacaacc ccaacaaaac caatacgttc acctttcaga taaatcgctg cggattgccc
      781 cggatgcagt gccggattcg cttctgcacg gaactcaacc tcattcagtt taccggtcag
      841 gtcgagaacg gattcaagat cgcctttcaa atcatagaaa tcaacggtct cttttgccag
      901 gttccagtgc tcttcgtaac ggttaccgca aatcacaccg gctaacatca gatcctgacg
      961 aatgcccaac ggtgcctgag tatctggtac gaaacgcaga ccgctttcga aaatgcgcac
     1021 acggttctgc tgacggttct ggttgtacac cacggttgcc agcaggccgg tccacagaga
     1081 aagacgcatt gctgacattt caacagagat cgggcttggc agcagtaagg cttcaacgcc
     1141 tggatggatc atctgctgca ctttcggatc aacgaagctg taggtgatca cttcctgata
     1201 gcctttgtcg ttgagcagcg ttttcacgcg cttgagcgac aggtcagctt cacggtgagt
     1261 acccataatc aggcttgcct gtaccggctc atccgggatg ttgttgtagc cgtaaacacg
     1321 cgcgacttct tcgaccagat cttcttcaat ctccatatcg aaacgccagc tcggcgcaac
     1381 tgcctgccac tcgtctttgc cttcggtcac ttcgcagccg agacgacgca gaatgtcagt
     1441 tacctgctca tccgcaatat gatggccgat caggcgatcc agtttgctac gacgtaaagt
     1501 gatggttgca cgcttcggca gcgttgcttc gttggtgata tcaattaccg gaccagcctc
     1561 accaccgcag atgtcgatca gcagacgggt cgcacgttcc atcgctttgt gctgcagtgc
     1621 cggatcaacg ccacgctcat aacggtgaga cgcatcagta tgcaggccat gacgacgagc
     1681 acgaccggtg atagacagcg ggctgaagaa agcgcattcc agcaagacgt tttgcgtttc
     1741 gtcattcacg ccagagtgtt cgccaccaaa gatgccgccc atcgccagcg ccttgttgtg
     1801 gtcggcgatg accagagtgt cagcattcag cttcgcttca gtgccatcaa gcagtaccaa
     1861 cgtttcgccc tctttcgcca tccgcaccac aatgccgcct tcaatgcgat ctttatcgaa
     1921 agcgtgcatc ggctggccca gttcgagcag tacatagttg gtgacgtcaa caactgcatc
     1981 gatagaacgg atcccgcaac gacgcagttt ttctttcatc cacagcggag ttggcgcttt
     2041 aacgttaatg ccttttacca cacggccaag ataacgcggg caggcttccg gcgcttcgac
     2101 tgtaatcggc agcgtgtcgt cgatggtcgc accaaccgga acgatttccg gttcaaccag
     2161 cggcagctga ttcagcacgg caacgtcacg cgcaacacca atgataccta aacagtcggc
     2221 acggtttggc gtcacgctga tttcgatggt gttgtcatca agtttcaggt attcacggat
     2281 gtcggtgcca atcggcgcat ccgcaggcag ctcgataatg ccgctatgat cgtcagaaat
     2341 tcccagctca gaaaaggagc acagcatccc ttcagacggt tcaccacgca gtttcgccgc
     2401 tttaattttg aaatcacccg gcagaacagc accaatggta gctaccgcca cacgcaggcc
     2461 ctgacggcag tttggcgcac cgcagacgat gtccagcagg cgatcgccgc caacattcac
     2521 ttttgtcaca cgcagtttgt cagcgttcgg atgctgcgca cactcaacca cttcaccaac
     2581 gaccacgccg tggaagctac cggcaaccgg ttctacaccg tcaacttcca ggcccgccat
     2641 agtgatttga ttcgccagcg catcgctatc aatcgccggg ttcacccatt cgcgtaacca
     2701 cagttcactg aatttcataa tctattcctg ccttatttaa actgtttgag gaaacgcaga
     2761 tcattttcga agaatgaacg caggtcggtg acgccgtaac gcaacatagt cagacgctcc
     2821 atccccatcc cgaaggcgaa accagagtaa acttccgggt cgatgccaac gttacgcagc
     2881 acgttcggat gcaccatccc gcaacccagc acttccagcc atttaccgtt tttacccatg
     2941 acgtcaactt ctgcagaagg ctcggtgaac gggaagtagg aaggacggaa gcgaatctgc
     3001 aaatcttcct caaagaagtt acgcaggaaa tcgtgcagag tgcctttcag gttggtaaag
     3061 ctgatgttgg tatcaacaat cagaccttcc atctgatgga acatcggcgt gtgagtctgg
     3121 tcgtagtcgt tacgataaac gcggccaggc gcaatgatac gaatcggtgg ctgctgggct
     3181 ttcatggtgc ggatctgtac gccagaggtc tgggtacgca gcaggcgggt agtgtcaaac
     3241 cagaaagtgt cgtggtcagc gcgcgccggg tggtgaccag gaatgttcag agcatcgaag
     3301 ttatgataat cgtcttcgat ttccggcccg gttgccacgg taaagccaag ctcaccgaag
     3361 aaactttcga tacggtcgat agtacgggta accggatgca gaccgccgtt ttcaatgcga
     3421 cgacctggca gagagacatc aatcgtttcc gccgccagac gcgcattcag tgcagcgctt
     3481 tccagttccg ctttacgcgc attcagcgcc tgctgaacct gctctttcgc ttcgttgata
     3541 accgcaccag ctgccggacg ctcttctggc ggcagctcac gcagggtcgt catctgaagg
     3601 gttaagtgcc cttttttacc caaatattcg acgcgcacat tatctaacgc ggcaacatct
     3661 gacgcctggc taatggccgc cttcgcactg gcaaccagtt ctgcgagatg tgacatggtt
     3721 ttcctcattg tgtcagtggt gacactggtt cgttggactt agagcctatc ccatcaggct
     3781 attttacttg ccattttggt cccgggcagt gctcgaaatt ctcacgtact taaatacgct
     3841 ccggtttctc cgcgctggcc gtgtccagac tggctgcgac aattacacct gatgagacag
     3901 gctttttatt tttcaaaacg cgcatacaaa aaaagcctcc actgggaggc tttcaggcgc
     3961 tgttttccgt ttctcttctc acgcgctagc ctcctggatt caggtgctaa agtaaaaaaa
     4021 gaagcggaaa atagcagcat tcattgcttg cgttaccttt tggtactctt caaaagacct
     4081 ttattgaaaa ggctacggcg ataaaagtca atgttttgat ggcgttgaaa cgaaaagagg
     4141 gagactagct ccctctttca actggcttat gccagagctg ctttcgcttt ttcaaccaga
     4201 gcggtgaacg ctactttgtc gaatactgcg atatcagcca ggatcttacg gtcgatttca
     4261 acagaggctt ttttcaggcc attgatgaat ttgctgtaag aaataccgtt ctgacgtgct
     4321 gctgcgttga tacgcgcaat ccacagttga cggaactgac gcttacgttg acgacggtca
     4381 cggtaagcat actgaccagc tttgataaca gcctggaagg caacgcggta tacgcgagaa
     4441 cgcgcaccgt agtagccttt agcttgtttc aaaattttct tgtgacgtgc gcgtgcaata
     4501 acaccacgtt ttacgcgagc catatgtgct ctcctgtatc tatattctaa ttaaaaagtt
     4561 aaaaacgtta acggcttatg cgtacggcag gcacgcgatt accaggccca gatcgccttt
     4621 ggaaaccatg gctttcggac gcaggtgacg tttacgtttg gtcgcttttt tggtcagaat
     4681 gtgacgcagg ttagcgtgct tgtgcttaaa accaccttta ccggtttttt tgaagcgctt
     4741 agcagcaccg cgtacggtct taatttttgg cattttaata acttccactt cgcattgtta
     4801 ataaacgaaa caaaggcgaa caaagcctgt gaagcccgaa ggctccacag acagtgctac
     4861 ttgaaggcct tactgtttct tcttaggagc gagcaccatg atcatctgac ggccttcgat
     4921 cttcgttggg aaggattcga ccactgccag ttcttgcaaa tcgtctttca cgcgattaag
     4981 cacttccata ccgatttgct ggtgcgccat ctcacgaccg cggaaacgca gcgtgatttt
     5041 ggctttatca ccctcttcga gaaagcgaat caggctgcgg agttttacct gatagtcgcc
     5101 ttcatctgta ccaggacgga atttaatttc cttaacctgg ataacttttt gctttttctt
     5161 ctgttcctta gaagacttgc tcttttcata gaggaatttg ccgtaatcca ttatacgaca
     5221 aaccggcggc tcggcgttag ggctgatctc gactaagtct actccggctt cttctgcttt
     5281 ctccagagct tctctcagac tcacaatacc aagctgctcg ccttccagac ctgttaagcg
     5341 aacttcctgg gcgcgaattt cgccattgat acggttaggg cgcgccgttt gaactcgttt
     5401 tccgccttta ataccttatt cctccaattg tttaagactg cggctgcgaa tctcttgttg
     5461 cagcttctcg atcacttcat ttacgtccat gcttcccagg tctttaccac ggcgggtgcg
     5521 aacggcaact ttgcctgatt ccacctcttt atcaccacag accagcatat atgggacgcg
     5581 acgcaaagtg tgctcgcgga ttttaaagcc aatcttctca tttctcaagt ctgctttaac
     5641 acgaatgccc gcatttgata gtttttgcgt caattcgtta acgtattcag actgtgaatc
     5701 ggtaatattc atgataacaa cctgaactgg cgcaagccag gtcgggaaga aaccagcgaa
     5761 ttcttcggtc aggataccga tgaaacgttc catcgatccc agaattgcgc ggtgaatcat
     5821 taccggtact ttacgttcat tgtcttcgcc tacataagaa gcgctcagac gagaaggcaa
     5881 agagaagtcc agctgtactg taccgcactg ccatgcacga tcgaggcagt catacagggt
     5941 aaattcaatt ttcggaccgt agaaagcgcc ttcacccagt tgatattcaa acgggatgtt
     6001 gttttcttcc agcgcaaccg ccaggtccgc ctcagcacga tcccacattt catcactgcc
     6061 aatacgtttt tcaggacgag tggagagttt gacgacgatc ttctcgaagc caaaagtgct
     6121 gtacatatca tagactaaac ggatacatcc gttaacttca tcgcgaattt gttcttcagt
     6181 acagaagata tgcgcgtcat cctgggtaaa tccacgcacg cgcatcaggc catgcagcga
     6241 acctgacggc tcattacggt ggcagctacc aaactcggcc atacgcagcg gcagatcgcg
     6301 ataagacttc agcccctggt tgaaaatttg tacgtgaccc gggcagttca tcggcttaat
     6361 gcagtattca cggttctcag aagacgtggt gaacattgca tctttgtagt tgtcccagtg
     6421 accggttttt tcccacagga cacggtccat catgaacgga cctttaactt cctgatactg
     6481 gtactctttc agtttagaac gaacaaacac ttctagttca cggaagatgg tccagccgtc
     6541 gttgtgccag aataccatac ccggcgcttc ttcctgcata tggtacaggt cgagctgttt
     6601 accgatttta cggtggtcgc gtttcgcggc ttcttccagg cgctgcaggt aagcgttaag
     6661 cgcttttttg tctgcccacg ccgtaccgta aatacgttgc aacattttgt tgttgctgtc
     6721 gccacgccag taagcccctg ccgttttcat tagtttgaaa tgatggcaga aacgcatgtt
     6781 cggtacgtgc ggaccgcggc acatatcgac atattcttca tggaagtaca gacctggctt
     6841 gtcatcatgg gcgatgtttt cgtcaagaat ggagactttg tagctctccc cacggttggc
     6901 gaaagtttca cgcgcttcgt gccagctgac tttcttctta atgacgtcgt agtttttctc
     6961 agcaagctca tgcatccgct tctcgagtgc ttcgacatct tcctgggtta acgtgcggtc
     7021 aagatcaacg tcgtaataaa aaccgttgtc aataaccggg ccgattgcca ttttggtatg
     7081 cggccaaagt tgtttaatcg cgtgccctaa caggtgcgca caggagtgac gaatgatctc
     7141 cagaccgtct tcgtctttgg cggtaatgat ggacagttgt gcgtcgtttt caatcagatc
     7201 gcaagcatca accagttcgc cattaacgcg ccctgcgata caggctttcg ccagacctgg
     7261 accaatgtcc agcgcaacat ccatggggct tacagcgtgg tcgtaatggc gttggctgcc
     7321 atcaggaaga gttataacag gcattttata tccttatttg cagtggtgac ccacacgaaa
     7381 gatcacatac aaagaaaaat ttgtttatta acagttcatt gcgaaaccat ctagccaaca
     7441 aatgctaaat tggttcgcaa tcaggtacac aaatgaaaga cattaacctc tcaccttcca
     7501 cccggttgag cgatgttaac actaaaaaaa gggagattgt acctttccgt ttcacattga
     7561 ttcgattcgc aattcgtttg tagtgagtac accagcaaaa cgcttaaaga accactgata
     7621 acatcacggt gcgtctgaaa ggtttactgc gagcggattt acagcagcat aaactgaatc
     7681 cagcaaagtt cgttgcgtga tttggcggta gtattttaaa cgctcatgag attaatgacg
     7741 aagtggtcat atcacaatga taaaagtgac acaattctta taacaatttt tcgtgcacat
     7801 ttcgttctgg cgataataat taatcatcat cctcaaaacc tccagatata tataaggcga
     7861 atagattatg agtcaaaacg atatcattat cagaactcat tataagtctc ctcatagatt
     7921 gcacatcgat agcgacatac caacaccttc atcagagcct attaataaat ttgcgcgcca
     7981 gctcatcacc ctacttgata cctctgactt aagttcgatg ctgtcatact gtgttactca
     8041 ggaatttacc gcaaactgtc gaaaaatatc acaaaattgt tattccactg ccctttttac
     8101 cattaacctt gccacttcac ccatccatgc agaaaatata ctcattacat tacactataa
     8161 aaaagatatc atttccttat tactggaaac cacgcctatt aaagctaacc atttgcgaag
     8221 catactggat tatattgaac aggaacagtt aactgctgaa gatcgtaacc attgtatgaa
     8281 actgtctaaa aaaatccata gagaaaaaac tatacaccca acagtaaatc tcaatggtag
     8341 tgcatgtttt ttgcaatctc cttctgacgc tattttttgt cgccatctgt cattgcaata
     8401 cgcccttgat tcattgagaa atggaaaagg caaagtcaac ctgattaaac attactcctc
     8461 cgttgaatcc atacagcagc atgtcccctt agtccgggac gcggagttca gagcattact
     8521 tcgccatcct cctgcaggga gtcgcgttat cgcgagtaag gattttggct tcgctttaga
     8581 ttttttcttt tgtcgaatga tggcaaacaa tatcagtcat atgtccgcga ttttatatat
     8641 agacaatcat actttgtcag taaggctacg aataaagcag tcagtgtatg gacaattaaa
     8701 ctatgttgtg tccgtttacg acccgaacga taccaacgtt gccgtcagag acacccacag
     8761 gacagcacgg ggctttcttt cgcttgataa gttcatcagt tcaggtcccg atgctcagac
     8821 ctgggctgat aggtatgttc gcaactgtgc aattgctatt ctgcccctat tacctgtggg
     8881 agttccaggg gctattttcg cgggtattgc atcacgaatg ccatttgccc ctatacatcc
     8941 atcggcaatg ttgttaataa tggctacagg ccagactcaa cagcttatta cattattcaa
     9001 acagttgccc atactccctg aaaaagaaat cattgaaata ataactgcgc agaatagcgt
     9061 tggtacacct gctttatttt tggctatgat gaacggacat actgacaacg tgaaaatatt
     9121 tatgcaagaa attcagtcac tggtagataa tcacatcatt catgaagata acctggttaa
     9181 attactgcaa actaaaagtg ctaacgaaac acctggactc tatatctcca tgttgtatgg
     9241 attcgatgaa ataatcgata tctttctgaa tgcattaacc actccaatag cacaagaact
     9301 tttaaacaaa aaaatggtga tggatatttt agccatgaaa acacgtgatg gtgaaccagg
     9361 gttatttgct gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc tctctaaagt
     9421 ttatgggatc gccgttaaat ataaactcag caaaatcaac atcatggact tattaaaagg
     9481 cgcgacagca cagggaaccc ctgcattata catcgccatg agcaagggta atgaagacgt
     9541 cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaaacattct tttagtcaac
     9601 atcagttatt tacactattg gccgctaaaa atcatgacaa catgtcagct gttcatatag
     9661 ccattcatca taagcattat aaaactgtag aaacatatta tgctgctata aatgtcatca
     9721 gccaaagcct gagttttagt gctgatgaaa taaagacgta tttataacca gctatttacg
     9781 atatatatcg atacataatt ttattttttt cataaaacgc agaaacagtg accaggtgta
     9841 caacgccacc caacggctgt aataacaaaa aatcccttga tgcccgtccc ttttgttaca
     9901 ctccgttatc acgcacaaga gatatgcagg acactggtat gccgactaaa cgctttgata
     9961 aaaaacactg gaagatggtg gtggtgctac tggcaatctg tggcgctatg ttgttgctac
    10021 gttgggcagc aatgatttgg ggctgagcag tgacaaacga agacagccag accgcataac
    10081 acggtccggc agataatttt acatgctgta gcccagcgat aaggttgtcc gacggtcggt
    10141 atgttccggt gcagactctg gaggttcaga gttccatgtg acgttgtaag ccaccttcag
    10201 tccaaaatgt tcattaatag caacatttaa tgcgctttcg gagttcagcg ttgtgtcttc
    10261 cgcgccaaag accgaaacac cctgcgtaaa tttagcgttg tcagtcaact gccatgcata
    10321 agccccggag gcatagccca gcggctgggt ttcactggca ttgtcggtat atttgtcgta
    10381 acgcacacct ggaccgaatt caaagcggaa actgtgtacc gggccattca aaaactgatg
    10441 accataaccc gcggtcaaca catcgcgctc tcgatagccg ttataacggt ctgtcagcca
    10501 gcttgcctga ccaaataaat agtcataatc agttaaatta aaacggctac gcccgcccgc
    10561 cgcatatttt tctgaagaac gctcatcatt agaagaagta ttactggcgt tcccccacag
    10621 cgaccaggca gtggtttgtc cataccaggt catggtggtg tcagccgtaa gtgaggagct
    10681 tttcgtattg cctgattgtg caagatatcc tgcgttcaga ttaccttcga aaggtttttt
    10741 agcgctggca ggatcgtcca tgacagtaaa aacggaatca tcggcagctg cattcagtga
    10801 cgcaaacatg ccccccgcca acataacgat ggcaggaact gtcttcaaaa gcttcattta
    10861 tcaagagtcc gtacaacaaa aaaagagacc atcgcggtcc cggaaacttt cttaaggatc
    10921 aaagattagc gtccctggaa aggtaacgaa ttataaaaag gcgcgaataa cttagcaatg
    10981 tattcttatt tcattttttg aataagcatg tggcgaaaac agatttttat ttatatatat
    11041 ttatctgcaa aattttaaat aaagctctaa taaatcatat tgttaatttc ttcactttcg
    11101 gctgattcgg tgccagactg aaatcagcct ataggaggaa atgatggtac gtatctatac
    11161 gttgacactt gcgccctctc tcgatagcgc aacaattacc ccgcaaattt atcccgaagg
    11221 aaaactgcgc tgtaccgcac cggtgttcga acccgggggc ggcggcatca acgtcgcccg
    11281 cgccattgcc catcttggag gcagtgccac agcgatcttc ccggcgggtg gcgcgaccgg
    11341 cgaacacctg gtttctctgt tggcggatga aaatgtcccc gtcgctactg tagaagccaa
    11401 agactggacc cggcagaatt tacacgtaca tgtggaagca agcggtgagc agtatcgttt
    11461 tgttatgcca ggcgcggcat taaatgaaga tgagtttcgc cagcttgaag agcaagttct
    11521 ggaaattgaa tccggggcca tcctggtcat aagcggaagc ctgccgccag gtgtgaagct
    11581 ggaaaaatta acccaactga tttccgctgc gcaaaaccaa gggatccgct gcatcatcga
    11641 cagttctggc gaagcgttaa gtgcagcact ggcgattggt aacatcgagt tggttaagcc
    11701 taaccaaaaa gaactcagtg cgctggtgaa tcgcgaactc acccagccag acgatgtccg
    11761 caaagccgcg caggaaatcg ttaatagcgg caaggccaaa cgggttgtcg tttcccttgg
    11821 tccacaagga gcgctgggtg ttgatagtga aaactgcatt caggtggtgc caccaccggt
    11881 gaaaagccag agtaccgttg gcgctggtga cagcatggtc ggcgcgatga cactgaaact
    11941 ggcagaaaat gcctctcttg aagagatggt ccgttttggc gtagctgcgg ggagtgcagc
    12001 cacactcaat cagggaacac gtctgtgctc ccatgacgat acgcaaaaaa tttacgctta
    12061 cctttcccgc taacaaaaac attcccccag cattggggga atcaccacca acctgtcggc
    12121 aacgcgtttc tccgactatg ctcaaaagtc atgtgataac aaaggggtga actatggcca
    12181 gtggcgatct tgtccgttat gtcataaccg tcatgttgca tgaggataca ttgactgaaa
    12241 ttaacgagtt gaataattac ctgactcgcg acggtttttt gctcaccatg acggatgatg
    12301 agggaaatat ccatgagctg gggactaaca cttttggact tatcagtacc caaagtgaag
    12361 aagaaattag agaactggtt tcggggctta cccaaagtgc aaccggcaaa gatcctgaaa
    12421 tcaccatcac gacctgggag gaatggaata gcaacagaaa ataaatggtt tttgggcaat
    12481 aatcagtctg tggtgtgcgt tagctcgtgt ttttacaccg cattcttgcg ctaaccttat
    12541 gatctggcag acaacatggg agagacatca tgtggcaggc aatcagtcgt cttttgagcg
    12601 agcagttagg tgaaggcgaa atcgaactgc gtaatgaact gcctggcgga gaagtccatg
    12661 ccgcatggca tttgcgctat gcaggacatg actttttcgt caaatgtgat gaaagggaac
    12721 tgcttcccgg ttttaccgcc gaagccgacc aactggagtt actgtcgcgt agtaaaaccg
    12781 tcaccgtgcc taaggtttgg gcagtaggcg ctgaccgtga ctacagtttt ctggtgatgg
    12841 attatctccc acctcgtccg ctggatgcgc atagcgcatt tattcttggt cagcaaattg
    12901 cgcgtttaca tcaatggagt gaccaaccac aatttggcct cgattttgat aactcgctct
    12961 ccacaactcc acagcccaac acctggcaac gtcgctggtc aacgtttttt gctgaacaac
    13021 ggattggctg gcagttggaa ctggcagcag agaaagggat cgctttcggc aatatcgacg
    13081 ccatcgtcga gcatattcag cagcgtctgg cctcgcatca accgcagcct tctctgttgc
    13141 acggcgattt atggtccggc aactgtgcgc tgggtccgga tggcccgtac attttcgacc
    13201 ctgcctgcta ctggggtgac cgagagtgcg acctggcgat gttaccgctg catactgaac
    13261 agccgccaca aatctatgac ggctatcagt cagtatcccc gctacctgcc gatttccttg
    13321 aacgtcaacc ggtttaccaa ctctacacgc tgttaaatcg tgcaagatta tttggcggtc
    13381 agcatttggt tattgctcag cagtcattgg atagattatt agcagcatga tatgggttga
    13441 ggataatggc cgctccgtgc ggccttttga ttaaataaac ccaagcagag aaaagaacac
    13501 ataacctgca gcaatacaaa taaccggcag gatatatagt gagaaaaact gcaggaaaat
    13561 agtatggtgc ggaacaacaa tacgggactc aatttgttca cgcgttagcc cctcttcccc
    13621 tttggctttt tccagaatga gttgatcttc aacaccttcc cgcaagaagc gcgcctggcg
    13681 actcatccgg gcaccggaat cctgcaacgc cagtccgaca aaaatcagga tgaaaatcac
    13741 ccagaacata acgttcacgc caccattaaa attgggcgtc ggggagttat accagaacag
    13801 attcaaaaaa ggcgtattcg cctgcatcat atcgatcatg acatgagtaa agtcgagcat
    13861 gaccgcatta atgccttcct ggttttcctg ccgggtattc ataaatttca gcaaagaaat
    13921 caatgtagag atcaatgcag gtataaaaat cacccaaccc aaaatccttt tcaaaacagc
    13981 aatgcgtcca gcttgttgat acgtcatgag ttctccttga ttaagacgcg tcgtttcact
    14041 tagtttacct gtagatatct gttttcgccc attctttaaa ggcgatatga taggcgctta
    14101 atcataagca cggcttaata ccttacacat aatgctctaa aggagaggtt gtaatgtcaa
    14161 ccccgcgtca gattcttgct gcaatttttg atatggatgg attacttatc gactcagaac
    14221 ctttatggga tcgagccgaa ctggatgtga tggcaagcct gggggtggat atctcccgtc
    14281 gtaacgagct gccggacacc ttaggtttac gcatcgatat ggtggtcgat ctttggtacg
    14341 cccggcaacc gtggaatggg ccaagccgtc aggaagtagt agaacgggtt attgcccgtg
    14401 ccatttcact ggttgaagag acacgtccat tattaccagg cgtgcgcgaa gccgttgcgt
    14461 tatgcaaaga acaaggttta ttggtgggac tggcctccgc gtcaccacta catatgctgg
    14521 aaaaagtgtt gaccatgttt gacttacgcg acagtttcga tgccctcgcc tcggctgaaa
    14581 aactgcctta cagcaagccg catccgcaag tatatctcga ctgcgcagca aaactgggcg
    14641 ttgacccact gacctgcgta gcgctggaag attcagtaaa tggcatgatc gcctctaaag
    14701 cagcccgcat gcgttccatt gtcgttcctg cgccagaagc gcaaaatgat ccacgttttg
    14761 tattagcaaa cgtcaaactc tcatcgctga cagaactcac cgcaaaagac cttctcggtt
    14821 aatgaccagg ggcagtgatc tcgctgcccc tggttcttta tctgaatttg cattcaactg
    14881 acggattaat cgtcaattta agagaaagag ttacaccgtc accacttccg tgcactgtat
    14941 aaaaatccta tactgtacgt atcgacagtt tagtgagttt tatcatgacg gcggaaggtc
    15001 accttctctt ttctattgct tgtgcggtat ttgccaaaaa tgccgagctg acgcccgtgc
    15061 tggcacaggg tgactggtgg catattgtcc cttccgcaat cctgacgtgt ttgttaccgg
    15121 acatcgatca cccaaagtcg tttcttgggc agcgattaaa gtggatatca aaaccgatcg
    15181 cccgcgcttt tgggcatcgt ggttttaccc acagtctgct ggcggtattt gcgctgctgg
    15241 caacctttta ccttaaggtt cctgaaagct ggttcattcc ggctgatgcg ctacaaggaa
    15301 tggtgctggg ttatttaagc cacatacttg ccgatatgct gacacccgcc ggtgttcccc
    15361 tgctctggcc atgtcgctgg cgtttccgct tgcctatcct ggttccccaa aagggcaacc
    15421 aactggaacg ttttatctgc atggcattat ttgtctggtc ggtatggatg ccccattcat
    15481 tacccgaaaa cagcgcggtt cgttggtcat cgcaaatgat caataccttg cagatccagt
    15541 ttcatcggct tattaagcat caggttgaat actaaaaagg caaaaatcac ctttctggaa
    15601 taagcaattc catttgaata taagagccag ctcacagttc tgttaatctt gcgccaacac
    15661 tatgactgct acgcagtgat agaaataata agatcaggag aacggggatg aactttccat
    15721 taattgcgaa catcgtggtg ttcgttgtac tgctgtttgc gctggctcag acccgccaca
    15781 aacagtggag tctggcgaaa aaagtgctgg tgggtctggt gatgggtgtg gtttttggcc
    15841 ttgccctgca taccatttat ggttctgaca gccaggtact taaagattct gtacagtggt
    15901 ttaacatcgt tggtaacggc tatgttcaac tgctgcaaat gatcgttatg ccgttagtct
    15961 tcgcctctat tctgagcgcg gttgcccgtc tgcataacgc atctcagtta ggcaaaatca
    16021 gttttctgac catcggtacg cttttgttta ccacactgat tgcggcgctg gtcggtgtgc
    16081 tggtcaccaa cctgtttggt ttgacggctg aaggtctggt tcagggtggt gcagaaactg
    16141 cacgtctgaa cgccatcgaa agtaactatg ttggtaaagt ctctgacctg agcgttccgc
    16201 agctggtctt gtcctttatc ccgaaaaacc cgtttgccga tctgaccgga gccaatccga
    16261 cgtcaattat cagcgtggta atttttgccg cattcctcgg tgtagctgcg ctgaaactgc
    16321 tgaaggatga tgcgccgaaa ggtgaacgcg tcttaaccgc tatcgatacc ctgcaaagct
    16381 gggtgatgaa actggttcgc ctggtcatgc agttgacccc ttacggcgtt ctggcactaa
    16441 tgaccaaagt ggttgcaggt tctaacctgc aagacatcat caaactggga agtttcgttg
    16501 tcgcgtccta cctcggtctg ctgattatgt ttgcagtgca tggcattctg ctgggcatta
    16561 atggcgtgag tccgctgaag tacttccgta aggtatggcc tgtgctgacg tttgccttta
    16621 ccagccgttc cagtgctgcg tctatcccac tgaatgtgga agcacaaacg cgtcgtctgg
    16681 gcgttcctga atccatcgcc agtttcgccg cctctttcgg tgcaaccatt ggtcagaacg
    16741 gctgcgccgg tttgtatccg gcaatgctgg cggtgatggt tgcgcctacg gttggcatta
    16801 acccgctgga cccgatgtgg attgcgacgc tggtcggtat tgttaccgtt agttccgcag
    16861 gcgttgccgg tgtcggtggt ggtgcaactt tcgccgcact gattgtgctg cctgcgatgg
    16921 gcctgccagt aaccctggtg gcgctgttaa tctccgttga accgcttatc gacatgggcc
    16981 gtacggcgct aaacgttagt ggctcgatga cagctggcac gctgaccagc cagtggctga
    17041 agcaaaccga taaagccatt ctggatagcg aagacgacgc cgaactggca caccgttaat
    17101 tcttatgctg gcaacggtcc gttttgtata ggggccgttg ctttactttt taaatgttcc
    17161 atcgcggcga tgcatactga ctcatattta aacagatcgt cagcgaaaaa aatgtccaac
    17221 ttatcaatac attcctggct caatgaatgt gcctgtagtg agaagcttaa aacctcatac
    17281 ccgagacgtt ttctaagtga actatctgga atactcataa tatcttcaag cacaccactt
    17341 cccattcccg gaagatttag tggattaaaa agaagattga tattaagcat tgatattatc
    17401 gaaggaaact ttaggagact ctcatttttt tt
//
LOCUS       DAZGOB010000101        17401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGOB010000101 DAZGOB010000000
VERSION     DAZGOB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.101"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..133)
                     /locus_tag="ACHLIF_003593"
     CDS             complement(<1..133)
                     /locus_tag="ACHLIF_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9607544.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     "
     gene            complement(544..1524)
                     /locus_tag="ACHLIF_003594"
                     /pseudo
     CDS             complement(544..1524)
                     /locus_tag="ACHLIF_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1821..2423
                     /locus_tag="ACHLIF_003595"
     CDS             1821..2423
                     /locus_tag="ACHLIF_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFO9607545.1"
                     /translation="MKNDKAWIGDLLGGPLMSRESRVIAELLLTDPDEQTWQEQIVGH
                     NILQASSPNTAKRYAATIRLRLNTLDKSAWTLIAEGSERERQQLLFVALMLHSPVVKD
                     FLAEVVNDLRRQFKEKLPGNSWNEFVNSQVRLHPVLASYSDSSIAKMGNNLVKALAEA
                     GYVDTPRRRNLQAVYLLPETQAVLQRLGQQDLISILEGKR"
     gene            2420..3022
                     /locus_tag="ACHLIF_003596"
     CDS             2420..3022
                     /locus_tag="ACHLIF_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFO9607546.1"
                     /translation="MIDPVLEYRLSQIQSRINEDRFLKNNGSGNEIGFWIFDYPAQCE
                     LQVREHLKYLLRHLEKDHKFACLNVFQIIIDMLNERGLFERVCQQEVKVGTETLKKQL
                     AGPLNQKKIADFIAKKVDLAAQDFVILTGMGNAWPLVRGHELMSALQDVMGFTPLLMF
                     YPGTYSGYNLSPLTDTGSQNYYRAFRLVPDTGPAATLNPQ"
     gene            3034..6675
                     /gene="brxC"
                     /locus_tag="ACHLIF_003597"
     CDS             3034..6675
                     /gene="brxC"
                     /locus_tag="ACHLIF_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFO9607547.1"
                     /translation="MNIEQIFEKPLKRNINGVVKAEQTDDASAYIELDEYVITRELEN
                     HLRHFFESYVPATGPERIRMENKIGVWVSGFFGSGKSHFIKILSYLLSNRKVTHNGTE
                     RNAYSFFEDKIKDALFLADINKAVHYPTEVILFNIDSRANVDDKEDAILKVFLKVFNE
                     RIGYCADFPHIAHLERELDKRGQYETFKAAFADINGSRWEDERDAYYFISDDMAQALS
                     QATQQSLESSRQWVEQLDKNFPLDINNFCQWVKEWLDDNGKNILFMVDEVGQFIGKNT
                     QMMLKLQTITENLGVICGGRAWVIVTSQADINAAIGGMSSRDGQDFSKIQGRFSTRLQ
                     LSSSNTSEVIQKRLLVKTDEAKAALAKVWQEKADILRNQLAFDPTTTTALRPFTSEEE
                     FVDNYPFVPWHYQILQKVFESIRTKGAAGKQLAMGERSQLEAFQTAAQQISAQGLDSL
                     VPFWRFYAAIESFLEPAVSRTITQACQNGILDEFDGNLLKTLFLIRYVETLKSTLDNL
                     VTLSIDRIDADKVELRRRVEKSLNTLERLMLIARVEDKYVFLTNEEKEIENEIRNVDV
                     DFSAINKKLASIIFDDILKSRKYRYPANKQDFDISRFLNGHPLDGAVLNDLVVKILTP
                     KDPTYSFYNSDATCRPYTSEGDGCILIRLPEEGRTWSDIDLVVQTEKFLKDNAGQRPE
                     QATLLSEKARENSNREKLLRVQLESLLAEADVWAIGERLPKKSSTPSNIVDEACRYVI
                     ENTFGKLKMLRPFNGDISREIHALLTVENDTELDLGNLEESNPDAMREVETWINMNIE
                     YNKPVYLRDILNHFARRPYGWPEDEVKLLVARLACKGKFSFSQQNNNVERKQAWELFN
                     NSRRHSELRLHKVRRHDEAQVRKAAQTMADIAQQPFNEREEPALVEHIRQVFEEWKQE
                     LNVFRAKAEGGNNPGKNEIESGLRLLNAILNEKEDFALIEKVSSLKDELLDFSEDRED
                     LVDFYRKQFATWQKLGAALNGSFKSNRSALEKDAAAVKALGELESIWQMPEPYKHLNR
                     ITPLIEQVQNVNHQLVEQHRQHALERIDARIEESRQRLLEAHATSELQNSVLLPMQKA
                     RKRAEVSQSIPEILAEQQETKALQMDADKKINLWIDELCKKQEAQLRAANEAKRAADS
                     EQTYVVVEKTVIQPVPKKTHLVNVASEMRNAAGGEVLETTEQVEKALDTLRTTLLAAI
                     KAGDRIRLQ"
     gene            6721..10338
                     /gene="pglX"
                     /locus_tag="ACHLIF_003598"
     CDS             6721..10338
                     /gene="pglX"
                     /locus_tag="ACHLIF_003598"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFO9607548.1"
                     /translation="MNTNNIKKYAPQARNDFRDAVIQKLTTLGIAADKKGNLQIAEAE
                     TIGETVRYGQFDYPLSTLPRRERLVKRAREQGFEVLVEHCAYTWFNRLCAIRYMELHG
                     YLEHGFRMLSHPETPTAFEVLDHVPEVAEALLPENKAQLVEMKLSGNQDEALYRELLL
                     GQCHALHHVMPFLFEAVDDEAELLLPDNLTRTDSILRGLVDNIPEEDWEQVEVIGWLY
                     QFYISEKKDAVIGKVVKSEDIPAATQLFTPNWIVQYLVQNSVGRQWLQTYPDSPLKDK
                     MEYYIEPAEQTPEVQAQLAAITPASIEPESIKVLDPACGSGHILIEAYNVLKNIYEER
                     GYRGRDIPQLILENNIFGLDIDDRAAQLSGFALLMMARQDDRRIFTRDVRLNIVSLQE
                     SLHLDIAKLWQQLNFHQQVQTGSMGDMFAENTALTQTNSAEYQLLMRTLKRFVNAKTL
                     GSLIQVPQEEEAELKVFLDALYRLEQEGDFQQKSAAKAFIPFIQQAWILAQRYDAVVA
                     NPPYMGSKYQSLSVKKHLKDNFTGYDKDLFSAFIVRNLELSKQGAQLGFMTPFVWMFI
                     TTYEELRKKLIEQEIITSLVQLEYSGFDGATVPICTFTLQKGHINHFTGSYIRLSDFR
                     GASNQGPKTLEAIQHHNCGWFYEAQPDNFKKIPGTPIAYWLGQNINIFDNDKIANSWF
                     SGGRIKTHNGDKYIRYIWETSRLSSKWKKLIKGGDYKKHFGNEIFLVDWSVNAIKFYE
                     KNGGIYPSKFRDKAGICWSKITSGQASFRVKSEDVEYDSASPVIFNDKFNSDLYTLAL
                     LNTKVFNVLLKALNPTINTQVNDVMSLPFIKMEAAVQEKIETNEQLLINIAMHDWNSQ
                     EISFDFMVNSLVSESRASSEVASSYAIARKKWQDAFNKTKELEEENNHLVITSSNFNG
                     INEELIDSEITLVSNPYYKYGDSTTNNETRLLSDSIADVLSYSIGCMMGRYSLDREGL
                     VYAHEGNKGFAELAAEGAYKTFPADNDGILPLMDDEWFEDDVTSRVKEFVRTVWGEEH
                     LQENLEFIAESLCLYAIKPKKGESALETIRRYLSTQFWKDHMKMYKKRPIYWLFSSGK
                     EKAFECLVYLHRYNDATLSRMRTEYVVPLLARYQANIDRLNDQLDEASGGEATRLKRE
                     RDSLIKKFSELRSYDDRLRHYADMRISIDLDDGVKVNYGKFGDLLADVKAITGNAPEA
                     I"
     gene            10515..13112
                     /gene="pglZ"
                     /locus_tag="ACHLIF_003599"
     CDS             10515..13112
                     /gene="pglZ"
                     /locus_tag="ACHLIF_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFO9607549.1"
                     /translation="MQNQDFIAGLKAKFAEHRIVFWHDPDKRFIEELEQLKLENVTLI
                     NMTHASQLAVKKRIEMDEPEQQFLLWFPHDAPPHEQDWLLDIRLYSSEFHADFAAITL
                     NTLGIPQLGLREHIQRRKAFFSTKRTQALKNLVTEQEDETSLDKKMIAVIAGAKTAKT
                     EDILFNLITQYVNQQTEDDSELENTQAMLKRHGLDPVLWEMLNHEMGYQAEEPSLENL
                     LLKLFCTDLSAQADRQQRAWLEKNVLLTPSGRASALAFMVTWRADRRYKEAYDYCAQQ
                     MQAALRPEDHYRLSSPYDLHECETTLSIEQTIIHALVTQLLEESTTLDREAFKKLLSE
                     RQSKYWCQTQPEYYAIYDALRQAERLLNLRNRHIDGFHYQDSATFWKAYCEELFRFDQ
                     AYRLFNEYALLVHSKGAMILKSLDDYIEALYSNWYLAELSRNWNEVLEAENRMQAWQI
                     PGVPRQQNFFNEMVKPQFQNPQIKRVFVIISDALRYEVAEELGNQINTEKRFTAELRS
                     QLGVLPSYTQLGMAALLPHEQLCYQPGNGDIVYADGLSTSGIPNRDTILKNYKGMAIK
                     SKDLLELKNQEGRDLIRDYEVVYIWHNTIDATGDTASTEDKTFEACRTAVTELKDLVT
                     KVINRLHGTRIFVTADHGFLFQQQALSVQDKTTLQIKPENTIKNHKRFIIGHQLPADD
                     FCWKGKVADTAGVSDNSEFLIPKGIQRFHFSGGAHFVHGGTMLQEVCVPVLQIKALQK
                     TAAEKQPQRRPVDIVAYHPMIKLVNNIDKVSLLQTHPVGELYEPRILNIYIVDNANNV
                     VSGKERISFDSDNSTMEKRVREVTLKLIGASFNRRNEYWLILEDAQTETGYQKYPVII
                     DLAFQDDFF"
     gene            13123..15207
                     /gene="brxL"
                     /locus_tag="ACHLIF_003600"
     CDS             13123..15207
                     /gene="brxL"
                     /locus_tag="ACHLIF_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFO9607550.1"
                     /translation="MQTHHDLPVSGVSAGEIASEGYDLDALLNQHFAGRVVRKDLTKQ
                     LKEGANVPVYVLEYLLGMYCASDDDDVVEQGLQNVKRILADNYVRPDEAEKVKSLIRE
                     RGSYKIIDKVSVKLNQKKDVYEAQLSNLGIKDALVPSQMVKDNEKLLTGGIWCMITVN
                     YFFEEGQKTSPFSLMTLKPIQMPNMDMEEVFDARKHFNRDQWIDVLLRSVGMEPANIE
                     QRTKWHLITRMIPFVENNYNVCELGPRGTGKSHVYKECSPNSLLVSGGQTTVANLFYN
                     MASRQIGLVGMWDVVAFDEVAGITFKDKDGVQIMKDYMASGSFSRGRDSIEGKASMVF
                     VGNINQSVETLVKTSHLLAPFPTAMIDTAFFDRFHAYIPGWEIPKMRPEFFTNRYGLI
                     TDYLAEYMREMRKRSFSDAIDKFFKLGNNLNQRDVIAVRRTVSGLLKLMHPDGAYSKE
                     DVRVCLTYAMEVRRRVKEQLKKLGGLEFFDVNFSYIDNETLEEFFVSVPEQGGSELIP
                     AGMPKPGVVHLVTQAESGMTGLYRFETQMTAGNGKHSVSGLGSNTSAKEAIRVGFDYF
                     KGNLNRVSAAAKFSDHEYHLHVVELHNTGPSTATSLAALIALCSILLAKPVQEQMVVL
                     GSMTLGGVINPVQDLAASLQLAFDSGAKRVLLPMSSAMDIPTVPAELFTKFQVSFYSD
                     PVDAVYKALGVN"
     gene            complement(15472..15897)
                     /locus_tag="ACHLIF_003601"
     CDS             complement(15472..15897)
                     /locus_tag="ACHLIF_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9607551.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGQHF
                     PGKKFCEACGTGIYSRAVCTKYVYSYQPDLFQDCGSAST"
     gene            complement(15894..16277)
                     /locus_tag="ACHLIF_003602"
     CDS             complement(15894..16277)
                     /locus_tag="ACHLIF_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9607552.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            complement(16649..17248)
                     /locus_tag="ACHLIF_003603"
     CDS             complement(16649..17248)
                     /locus_tag="ACHLIF_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9607553.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT         4770 a         4124 c         4196 g         4311 t
ORIGIN      
        1 aagtgaactg tagttgccat gttttacggc aatgagagca gagatagcgc tgatgtccgg
       61 cagtgctttt gccgttacgc accacgcctt cagtagcgga gcaggaagga catctgatgg
      121 aaatggaagc cacgcaagca ccttaaaatc accatcatac actaaatcag taagttggca
      181 gcattaccag cagatgtaaa gaataccgct ttgttaatct gacttgtaga cttctttggg
      241 attgtatcac tttcactcat aatcatttat tccattaatt aaattcgcac tggatagaag
      301 gtactacctc tcacagagta gcaaagttca actggctatg cggcgttaat cggaatgcat
      361 ggtggaatgt gaatcgccac cggtttttta gggggttgta gagtggcatc ggaaagaggt
      421 ttttaggggg ggaggttgat aataccgcgc ccccataatc ttgccatctc ttacattgag
      481 atgttaaaag ctacaaatgc tctccctatt tttcaatgta gacatttata gcaatataaa
      541 aatttattgt tcagcttttt caacaacaaa accattttgc caaacaaaca caatcaagag
      601 ttcaatatta acaatacccc attgattaga aatggcgaca atttcaccag aaggaagtgt
      661 aatcaatgca tcttcgttga tatgatagcg actcttttgc ttctctggta tctgatcgat
      721 aagtataacc gtacgttttt taaagtcttc acttaatcca tttattaaat cattataact
      781 tgcagggtta aattgtttta tacaatcgcg taataactca agcgctaact tacgtttatt
      841 aagtttttta tctttgcata tataccacgt atagcccttc tcttttttct gactgctaat
      901 tagctgctca tcacgttttt cacgaaactt aacttgatat tcttccaatt caggaaccgg
      961 tatgatttgc tctgcattga tcagaacatc atcattaaat ctataaggcg tcagacgaac
     1021 acatcgaatg tctacccctt tatctcttaa ccaaatcgca gtggtagtca gttccttgct
     1081 aaaatccgat gaagccaaaa cgattctgat atctttacca aaatcaacaa gctcagtttc
     1141 atccagctca acaaattcaa gtattttttc tttcgcattt tttctttcgc atcggcatcg
     1201 cggttttgtt ttttcaaata cgtctgaaaa tactcacatg ctttcgcaaa actcattgtg
     1261 taaatcattg ccgcatatcg tagtgcctgt aactccatat gcgtccccgt ttcatcacgt
     1321 tttaattcga taaccactat atttgcctgt ttatcgattg ccaataaatc aattcgccga
     1381 tggctatctt cccagtcaga aatcaccaga caatcaggag caataatatc tatatttttt
     1441 gattgcttcc tgtaatcctt ccagagtaaa ggtcgttttt tctaaagcat gtggttcttt
     1501 atttgcgata ttgtacagca ccacgttcat cttccttttt taactgattt tacagagact
     1561 ttaatacagt taaaatttta tttcctgagc tgtaatcgat taagttgatg catttaatgg
     1621 gaatgatata gggtcatttc cagtctcact tatagaaatg gctaaagcat gactctcgcc
     1681 aaaaccgttt atgtgttgta cataacgcga tcatccctct cacaaattgc cttttctcat
     1741 ggcatctcgc ccggtccccc attacaatca ctttttgttt tttgcgagct gcattccagt
     1801 cttcagaggg tttttcgatg attaaaaatg acaaggcatg gataggagac ttgctgggcg
     1861 gaccgctcat gagcagggaa agccgcgtca ttgccgaact gttgctaacc gatcccgatg
     1921 aacagacatg gcaagagcaa attgttggcc acaacatttt acaagcctct tctcctaaca
     1981 ccgcaaaacg ttacgcggca acaatcaggc ttcgcctgaa cacgctggat aaaagcgcgt
     2041 ggacattgat tgccgaaggt agtgaacggg aacgccaaca acttctgttt gtggctctga
     2101 tgctacattc gccggtagtt aaggattttc tggctgaagt ggtgaacgat ctgcgcaggc
     2161 agttcaagga aaagttgcct ggcaatagct ggaacgaatt tgtgaatagc caggttcgcc
     2221 tacatccggt actcgccagc tactcagatt catctattgc aaaaatggga aacaatctgg
     2281 tgaaggcgct tgctgaagcg ggttatgtgg atacgccccg cagacgtaac ctgcaggcag
     2341 tttacctttt accggaaact caggcagtgt tacagcgcct gggacaacag gacttgatat
     2401 ctattctgga gggaaaacgg tgatagatcc cgttcttgaa tatcgcctgt ctcaaatcca
     2461 gagtcgcatt aacgaagatc gcttcctcaa aaataacggc tccggaaatg aaattggttt
     2521 ttggatcttt gattatcccg cgcagtgcga actgcaggta cgggagcatt tgaaatatct
     2581 gctccggcat ctggaaaagg accataaatt tgcctgtctg aatgtcttcc aaatcatcat
     2641 cgatatgctc aatgaacgcg gccttttcga gcgcgtctgc cagcaggaag tcaaagtggg
     2701 tactgagacg ctgaaaaagc agcttgctgg tccgttaaat cagaaaaaga tcgctgattt
     2761 tatagcgaaa aaagtcgatc tggctgccca ggattttgtc attcttaccg gcatgggcaa
     2821 cgcctggcca ttagtacgcg gtcatgaact gatgagtgcc ttgcaggatg tcatggggtt
     2881 caccccactg ctgatgtttt atcctggcac ctacagcggg tacaaccttt ccccgctcac
     2941 agacaccggt tcacaaaatt attatcgcgc tttcagactg gtaccagata cgggacccgc
     3001 agcaacattg aatcctcaat gaagagcata acaatgaata ttgaacagat ttttgaaaaa
     3061 cctctaaaac gaaatataaa cggggtagtc aaagcagagc aaaccgatga tgccagcgcg
     3121 tacatcgagt tagatgaata tgtcatcacc cgcgaactgg aaaaccatct tcgccatttc
     3181 ttcgaatcct atgttcctgc cactggcccg gaacggatcc gtatggaaaa caagatcggc
     3241 gtatgggttt caggcttctt cggttcaggt aaatcgcact ttattaagat tctttcttat
     3301 cttttatcta accgcaaagt tacacataac ggtacggaac gtaatgctta ctccttcttt
     3361 gaagataaaa tcaaagatgc attattcctt gccgatatta acaaagcggt gcattacccg
     3421 actgaagtca ttctgttcaa tattgattcg cgtgccaacg tagatgacaa agaagatgcc
     3481 attcttaaag tcttcctgaa agttttcaac gaacgcattg gatactgcgc tgattttccg
     3541 catattgccc atcttgagcg cgagctggat aaacgcggtc agtatgaaac ctttaaagcc
     3601 gcgtttgccg atatcaatgg ctcgcgctgg gaagacgagc gcgacgctta ctacttcatc
     3661 agcgatgaca tggcacaagc attaagccag gccacgcagc agagtcttga atcctcccgc
     3721 caatgggtgg aacaactcga caaaaacttc ccgctggata tcaataattt ttgccagtgg
     3781 gtaaaagagt ggctggatga caatggtaag aacatcctct ttatggtgga tgaagtcggt
     3841 cagttcattg gcaaaaatac gcaaatgatg ctgaagctgc agactattac tgaaaacctt
     3901 ggggtaattt gcggtggccg cgcatgggtt atcgtgactt cgcaggccga tatcaacgcg
     3961 gcaatcggtg gtatgagcag tcgcgacgga caggacttct ccaagatcca ggggcgcttc
     4021 tctacacgcc tgcaactttc cagctctaac acatcagaag ttatccagaa acgtttgttg
     4081 gtaaagactg acgaagcaaa agcggcactg gcaaaagtgt ggcaagagaa agccgatatc
     4141 ctgcgtaacc agctggcttt tgaccctaca acaactactg cactacgtcc ttttaccagc
     4201 gaagaagagt tcgttgacaa ctacccgttt gtcccgtggc actatcagat tctgcaaaaa
     4261 gtgtttgaat ctattcgaac gaaaggtgca gcgggtaaac aattggccat gggtgagcgt
     4321 tctcagctgg aggcattcca gacggcggcg cagcaaatct cagcgcaagg gctggattct
     4381 ctggtgcctt tctggcgctt ctatgccgcc attgagagct tcctggaacc tgccgttagc
     4441 cgcaccatca ctcaggcttg ccagaatggc attcttgatg aattcgatgg caacctgctt
     4501 aaaacgctgt tcctgatccg ctatgtggaa acgctgaaaa gcaccctgga taacctggtc
     4561 acattgtcta tcgataggat cgatgccgat aaagttgagt tgcgccgccg ggtcgaaaaa
     4621 agtctgaaca cgcttgaacg cctgatgctc attgcgcgcg ttgaagataa atatgtgttc
     4681 ctgaccaacg aagaaaaaga gatcgaaaac gagatccgta acgttgatgt cgatttctct
     4741 gcgatcaaca aaaaactggc atcgatcatc tttgatgaca ttctgaaaag ccgtaaatat
     4801 cgttatccgg ctaacaagca agactttgat atcagtcgct tcctgaacgg acacccatta
     4861 gacggcgcag tgcttaacga tctggtagtg aagatcctga cccctaaaga tccgacctat
     4921 tcgttctata acagcgatgc gacctgtcgc ccttatacgt cagaaggcga cggctgtatt
     4981 ttgattcgtc tgcccgaaga gggccgtacc tggagcgata ttgatttagt cgtccagact
     5041 gaaaagttcc tcaaagataa cgccgggcaa cgtccggaac aggcaaccct gctctcagaa
     5101 aaagcgcgtg aaaacagcaa ccgggaaaaa ttactccgtg ttcagttgga atcactactt
     5161 gcagaagcag atgtctgggc gattggcgag cgcttaccga aaaaatcttc cacaccatcg
     5221 aacattgtcg atgaagcctg ccgttacgtg attgaaaaca ccttcggcaa gctgaagatg
     5281 ctgcggcctt ttaacggcga catctcccgt gaaattcatg cattactgac ggttgagaac
     5341 gacaccgaac tggatctcgg taacctcgaa gagtccaacc ccgacgccat gcgcgaggta
     5401 gaaacctgga tcaacatgaa tatcgaatac aataaacctg tgtatttacg cgatattctg
     5461 aaccatttcg cgcgtcgccc ttatggctgg cccgaagacg aagtgaaact gctggtagcc
     5521 cgtctggcct gcaaaggtaa gttcagcttt agccagcaaa acaacaacgt cgagcgaaaa
     5581 caggcgtggg agttatttaa taacagccgc cgccatagcg aattgcgtct gcataaagtt
     5641 cgccgtcatg atgaagcgca ggtgcgtaaa gccgcgcaaa ccatggctga catcgctcag
     5701 cagccgttta acgaacggga agagccggcg ctggttgaac atattcgtca ggtatttgaa
     5761 gagtggaagc aagagctgaa cgtattccgc gccaaggcag agggcggaaa caatccgggg
     5821 aaaaacgaga ttgaatccgg tctgcgcctg cttaatgcca ttcttaatga gaaagaagat
     5881 tttgccctga tcgaaaaagt ctcatcgctg aaagatgaac ttctggattt cagtgaagac
     5941 cgtgaagatt tggtcgactt ctaccgtaag caattcgcca cctggcaaaa actgggtgct
     6001 gcgctgaatg gcagctttaa atctaaccgc agcgcgctgg aaaaagacgc cgcagcggtt
     6061 aaagcgctgg gtgagctgga aagcatctgg caaatgccgg aaccttataa gcatctcaat
     6121 cgcatcacgc cgttgattga acaggtccag aacgtcaacc atcagttagt cgaacagcac
     6181 cgccagcacg ccctcgaacg cattgacgcc cgcattgagg aaagccgtca acgcttgctg
     6241 gaagcgcacg ccacgtcaga actgcaaaac agcgttctgc tgccgatgca aaaagccaga
     6301 aaacgcgctg aggtcagcca gtcgattccg gaaattttgg cggaacagca agagacaaaa
     6361 gcgctgcaaa tggatgcaga taaaaagatt aatctgtgga tcgatgagct gtgtaaaaag
     6421 caagaagcac aactccgggc agcaaatgaa gctaaacgcg ctgccgactc agaacagact
     6481 tatgttgtgg tggaaaaaac cgttatccaa ccggtaccga aaaaaacgca tctggtgaat
     6541 gtcgccagtg agatgcgtaa cgccgccggt ggtgaggttc tggaaaccac cgaacaggtg
     6601 gaaaaggcgc tcgacacgtt acgcacaacg ctgctggccg ccattaaagc aggcgatcga
     6661 attcgccttc agtaactccc atttcagggc agcactctgc tgccctttgc aggattttct
     6721 atgaatacta ataacattaa aaaatatgcc ccacaggccc gtaacgattt ccgcgatgcg
     6781 gtgattcaga agctaacgac gcttgggatc gctgcagata aaaaaggcaa tttgcagatt
     6841 gccgaggccg aaaccattgg cgagaccgtg cgttacggtc agtttgatta cccgttatcg
     6901 acccttcccc gccgcgaacg gctggtaaaa cgcgcccgtg agcagggttt tgaggtgctg
     6961 gttgagcact gcgcctacac ctggtttaac cgcttatgtg caattcgcta tatggagcta
     7021 cacggttatc ttgagcacgg cttccgtatg ttgtcccacc cggagacgcc gaccgcgttt
     7081 gaggtgctgg atcatgtgcc ggaagtggca gaagccctgc tgccggaaaa taaggcgcag
     7141 ctggttgaaa tgaagctttc cggtaatcag gacgaagccc tgtaccgcga actgctgctg
     7201 gggcagtgcc acgccctgca ccacgtgatg ccgttcctgt ttgaagcggt agatgacgaa
     7261 gcggaactgc tgttgccgga taacctgacc cgtaccgact ctattctgcg tgggctggtt
     7321 gataatattc ctgaagaaga ctgggagcag gtagaggtta tcggctggct gtatcagttc
     7381 tatatttcgg aaaagaaaga tgctgtgatt ggcaaagtgg tgaagagcga agatattcct
     7441 gccgccaccc agctgtttac gccaaactgg attgtgcagt atctggtaca gaactccgtt
     7501 ggccgccagt ggttgcagac ctacccggac tcgccgctga aagacaaaat ggagtactac
     7561 attgagcctg cggagcaaac gccggaagtg caggcacagc tggcggcgat taccccagcc
     7621 agcattgaac ccgaaagtat taaagtgctc gacccggcct gcggttccgg tcatattttg
     7681 attgaagcct ataatgtgct gaaaaatatc tacgaagagc gtggttatcg cgggcgtgat
     7741 attccacaac tgattctgga aaataatatt tttggtcttg atatcgacga ccgcgcagcc
     7801 caactttccg gttttgcatt attaatgatg gcgcgtcagg atgaccgcag aatatttacc
     7861 cgcgatgtac gtctgaatat tgtctctttg caggaaagcc tgcatctgga tatcgccaaa
     7921 ctctggcagc aactgaattt ccaccagcag gtacaaaccg gcagtatggg ggatatgttt
     7981 gctgaaaata ccgcgttaac ccaaactaac agcgcagaat atcagctgct gatgcgcacg
     8041 ttgaaacgct ttgtgaatgc aaaaacgctg ggatcgctga ttcaggtgcc gcaggaagaa
     8101 gaagcggaac tgaaagtatt tctggacgcg ctgtatcgcc tggaacagga aggcgatttc
     8161 cagcagaagt cggcggcgaa agcgtttatt ccgtttattc agcaggcgtg gattctggca
     8221 cagcgatatg atgcggtggt ggcgaatccg ccgtatatgg gaagcaaata ccaatcatta
     8281 agtgtaaaaa aacatcttaa agataatttt actggttatg ataaggattt attttcagcc
     8341 tttatagtca gaaacttaga actttcaaaa caaggggctc aacttggatt catgacgcca
     8401 tttgtctgga tgtttatcac tacgtacgaa gaattaagaa aaaaactgat agagcaagaa
     8461 attattacat cgcttgtgca gcttgaatat tcaggctttg acggtgcaac tgtgccaatt
     8521 tgcacattca cattacagaa aggtcatatt aaccatttta ctggtagcta tatccgcctc
     8581 tctgattttc gtggtgcatc aaaccaaggt cccaaaactc ttgaagcgat acaacaccat
     8641 aattgtggat ggttttatga agcgcagcca gacaatttta aaaaaatacc aggaacacca
     8701 atagcctatt ggctaggcca gaatataaat attttcgata atgacaaaat agctaattca
     8761 tggttctctg gtggtagaat taaaactcac aatggtgata aatatataag atatatatgg
     8821 gaaactagtc gtctttcgag taaatggaaa aaactaatca aaggtggaga ttacaaaaaa
     8881 cattttggca atgaaatctt tctggtagat tggtccgtaa atgctataaa attttatgaa
     8941 aaaaacggtg gcatttatcc atcaaaattt agagataaag ctggtatttg ttggtctaaa
     9001 atcacatcgg gccaagctag ctttagagtc aaaagtgaag atgtagaata cgattctgca
     9061 tcacctgtaa tatttaacga taaatttaac agtgatcttt atacgcttgc tctcttgaat
     9121 acaaaagttt ttaatgttct acttaaagca ttgaacccta ctattaatac ccaagttaat
     9181 gatgtcatgt cattaccatt catcaaaatg gaagcagctg ttcaagaaaa aatcgaaact
     9241 aatgaacagc tgctgataaa tatagcgatg cacgattgga attcgcaaga aatttctttt
     9301 gattttatgg ttaattcttt agtcagcgaa tcgagagcat catctgaagt agcatcaagc
     9361 tatgctatag caagaaaaaa gtggcaagat gcttttaata aaacgaaaga gttggaagaa
     9421 gaaaataacc atttagtcat tacaagtagc aactttaatg ggataaatga agaattaatt
     9481 gattcagaaa taactttagt cagcaaccca tactacaagt acggtgattc aacgactaat
     9541 aatgaaactc gtttgttatc ggattcaata gcagatgtat taagttattc catcggctgc
     9601 atgatgggtc gctactccct cgatcgcgaa ggcctcgtct acgcccatga aggcaacaaa
     9661 ggctttgccg aacttgccgc tgaaggtgcg tacaaaacct tcccggcaga caatgacggc
     9721 atcctgccgc tgatggatga cgagtggttt gaggacgacg tcacctctcg cgtcaaagag
     9781 tttgtccgca ccgtctgggg cgaagagcac ctgcaggaaa acctcgaatt tatcgccgaa
     9841 agtctctgtt tatacgcgat caagccgaaa aaaggcgaat ctgcgctgga gaccattcgt
     9901 cgctatcttt ccacacagtt ctggaaagat catatgaaga tgtataaaaa gcgcccaatc
     9961 tactggctat tcagctccgg taaagagaaa gccttcgagt gcctggtgta tctgcatcgc
    10021 tataacgacg ccacgctgtc gagaatgcgt accgaatatg tggtgccgct gctggcacgt
    10081 tatcaggcca atatcgatcg cctgaacgat caacttgatg aagcttctgg cggtgaagcc
    10141 acacgcctga aacgcgaacg cgacagcctg atcaaaaaat ttagcgaact gcgtagctat
    10201 gacgaccgcc tgcgtcacta tgctgatatg agaatcagta ttgatctcga cgatggcgtt
    10261 aaggttaact acggcaagtt tggcgatctg ctggcagatg tcaaagccat caccggcaat
    10321 gccccagagg cgatctaaac cagacggcgc gttcccctac tgccgggttc tgcccggtgg
    10381 caaataccac cgggaaacgc gccgctgctg gcatttctcc acctcacttc atgataaaat
    10441 gcgccaccgt gtcaaaatct ccttttcgcg ttttggcgct ttcttattca tcgtaacaac
    10501 atgggattgt gaacttgcaa aatcaggact ttattgctgg ccttaaagct aaatttgccg
    10561 aacatcgcat cgttttctgg cacgatcccg ataaacgttt tattgaggaa ctggaacagc
    10621 tcaagcttga aaacgtcacg ctaatcaaca tgacccacgc gtcacagctg gcggtgaaaa
    10681 aacgcatcga gatggatgag ccagaacagc agttcctgct gtggttcccc catgatgcgc
    10741 cgcctcatga acaagactgg ctgctggata tccgccttta cagcagcgaa ttccatgccg
    10801 attttgccgc catcaccctg aacacgctgg gcattcccca gcttggcctg cgcgagcata
    10861 ttcagcgacg caaggccttc ttcagcacta aacgtacgca ggcgctgaaa aacctggtga
    10921 cggaacagga agatgaaacc tcgctggata agaaaatgat tgcggtgatc gctggcgcaa
    10981 agaccgcgaa aaccgaagac attttgttta acctgattac ccagtacgtt aaccaacaaa
    11041 cagaagacga cagcgaactg gaaaacacgc aggcgatgtt gaaacgccac ggtctggacc
    11101 cggtattgtg ggaaatgctc aaccacgaaa tgggctacca ggcagaggag ccatcgctgg
    11161 aaaacctgct cctgaaactg ttttgtaccg atctctctgc ccaggccgac agacagcagc
    11221 gcgcctggct ggaaaaaaat gtcctgctga cgccatccgg cagagcatcc gccctggcat
    11281 ttatggtgac ctggcgtgcc gatcgtcgct ataaagaggc ttatgactac tgcgctcagc
    11341 aaatgcaggc ggccctgcgc ccggaagatc attaccgact cagttcgccg tatgatttgc
    11401 acgaatgcga aaccaccctc agcatcgaac aaaccattat tcatgcgctg gtaacgcaac
    11461 tgctggaaga gagcaccacg ctcgatcggg aagcctttaa aaaactgctc tctgaacgcc
    11521 agagcaaata ctggtgccag acacaaccag agtattacgc catctatgac gcgctgcgcc
    11581 aggctgagcg tttgctgaat ctgcgcaatc gccacatcga cggtttccac tatcaggaca
    11641 gcgccacctt ctggaaagcc tactgcgaag aactgttccg cttcgaccag gcttatcgcc
    11701 tgtttaatga atatgccctg ctggttcaca gcaaaggggc gatgatcctc aagagcctgg
    11761 atgattatat cgaggcactc tacagcaact ggtatctggc agaattaagc cgtaactgga
    11821 atgaagtgct ggaagcggaa aatcgtatgc aggcgtggca aattcctggc gtgccgcgtc
    11881 agcagaactt cttcaatgag atggtgaagc cgcagttcca aaatccgcaa atcaaacgcg
    11941 tgttcgtgat aatttccgat gccctgcgtt atgaagtggc ggaggagctg gggaatcaaa
    12001 tcaataccga gaaacgcttt accgcagaac tgcgatcgca gctcggcgtg ctccctagct
    12061 acacccaact gggaatggcg gcgttgctgc cccatgaaca actttgctat caacccggta
    12121 acggcgacat cgtttatgct gatgggctgt cgacctcggg tattcctaac cgcgatacca
    12181 ttctgaagaa ctataaggga atggcgataa aatcgaagga ccttctggag ttaaaaaacc
    12241 aggaagggcg agaccttatt cgcgattacg aagtagtgta tatctggcat aatacgattg
    12301 atgccactgg cgacacggca tccacggaag ataaaacctt cgaagcgtgc cgcacggcgg
    12361 tgactgaact gaaagattta gtcaccaagg taatcaaccg tctccacggc acacgcattt
    12421 ttgttacggc ggatcacggt ttcctgttcc agcaacaggc gctttcggtt caggataaaa
    12481 ccactctgca aattaagccg gaaaacacca tcaagaacca caaacgcttt attatcggcc
    12541 accagcttcc cgccgatgat ttttgctgga aagggaaagt ggcggatacc gcaggcgtga
    12601 gcgacaacag cgagttcctt attccgaaag ggatccagcg cttccatttc tctggcggcg
    12661 cgcacttcgt tcatggcggc accatgttgc aggaggtttg cgttccggta ttgcagataa
    12721 aagccctgca aaaaaccgcc gcagaaaaac agccgcagcg ccgcccggtg gatattgtcg
    12781 cttaccatcc gatgattaag ctggtgaaca atatcgataa agtgagcctg ttgcagacgc
    12841 atccggtggg cgaactttat gaaccgcgta tcctgaacat ttacattgtc gacaacgcca
    12901 acaatgtggt ctcgggcaaa gagcgcatca gctttgacag cgataacagc accatggaaa
    12961 aacgcgtacg cgaagttacg ctgaagctga ttggcgctag cttcaaccgt cgcaatgagt
    13021 actggttgat actggaagac gcacaaacgg aaacggggta tcagaagtac ccggtcatta
    13081 tcgatctggc gttccaggat gatttcttct aagtgaggcg atatgcaaac ccatcatgat
    13141 ttacctgttt caggcgtatc cgcaggggaa attgcctccg agggttacga tctggacgcc
    13201 ctgctgaacc agcattttgc tggtcgcgtg gtgcgtaaag atctcaccaa gcaactcaag
    13261 gaaggggcaa acgtcccggt gtatgtgctt gagtatctgc tcggcatgta ctgcgcctct
    13321 gacgatgacg acgtggtcga gcaagggttg caaaacgtta agcgtattct ggctgataac
    13381 tatgtgcgcc cggatgaagc ggagaaagtg aagtcgctga tccgcgagcg tggttcgtac
    13441 aaaatcatcg ataaagtgtc ggtgaaactg aaccagaaaa aagacgttta cgaagcccag
    13501 ctttctaacc tcggcatcaa agacgcgctg gtgccctcgc agatggttaa agacaacgag
    13561 aagctactga cgggcggtat ctggtgcatg attaccgtca actatttctt tgaagaaggg
    13621 cagaagacct cacccttctc attgatgacg ctcaagccta tccagatgcc gaatatggat
    13681 atggaagaag tgttcgatgc gcgtaaacac tttaaccgtg accagtggat cgatgtgctg
    13741 ctgcgctcgg tgggtatgga gcccgccaat attgagcaac gcaccaaatg gcaccttatc
    13801 acccgtatga tcccgttcgt ggagaacaac tataacgttt gcgagctggg gccgcgtggc
    13861 accggtaaaa gccatgtgta taaagagtgt tctcctaact ccctgttagt ttccggcggg
    13921 caaacgaccg ttgccaactt gttctacaac atggccagtc gccagatcgg cctggttggc
    13981 atgtgggatg tggtagcgtt cgacgaagtc gcggggatca ctttcaaaga taaagacggc
    14041 gtgcaaatca tgaaagatta catggcgtca ggatctttct ctcgcggcag agattcgatt
    14101 gaaggtaaag cgtcgatggt tttcgtcggc aacatcaatc aaagcgtaga gactctcgtt
    14161 aaaaccagcc atttgctggc accatttccg actgcgatga ttgatacagc atttttcgac
    14221 cgctttcatg cctatattcc cggttgggaa atccccaaaa tgcgcccgga attctttacc
    14281 aaccgttacg ggctgattac ggattatctc gctgaatata tgcgcgaaat gcgcaaacgc
    14341 agtttctctg atgcgattga taaattcttt aagctgggta acaacctcaa ccagcgtgac
    14401 gttattgccg ttcgacgtac cgtgtcgggg ttgttaaaac tcatgcatcc cgatggcgcg
    14461 tacagcaaag aagatgtgcg agtctgcctg acctatgcga tggaagttcg ccgccgcgtg
    14521 aaagagcaac ttaaaaaact gggcggtctg gagttcttcg atgtgaactt tagctacatc
    14581 gacaacgaaa cgctggaaga gttttttgtg agcgtaccgg aacagggcgg cagcgaactt
    14641 attcctgccg gaatgccaaa gccgggtgtt gtgcatctgg tcactcaggc agaaagcggc
    14701 atgaccgggc tgtatcgttt tgaaacacag atgactgccg gtaatggtaa gcatagtgta
    14761 tcgggtctgg gttcaaatac ctccgcgaaa gaagctatcc gcgtcggttt cgattacttc
    14821 aaaggcaatt tgaatcgggt aagcgcggcc gcgaaattct ccgatcatga atatcacctt
    14881 catgtcgttg aactgcataa tactggccca agcaccgcaa ccagtcttgc tgcgcttatc
    14941 gctttatgtt cgatattgct ggcaaaaccg gtgcaggaac agatggtggt gttgggcagt
    15001 atgacgcttg gtggggtaat taacccggtg caggatcttg ccgccagttt gcagctcgcc
    15061 ttcgacagcg gtgcaaaacg ggttctgttg ccgatgtcct cggctatgga tattccaacg
    15121 gttccggcag agttatttac caagtttcag gtgagttttt actcagaccc ggttgatgct
    15181 gtttataagg cgctgggtgt gaattaacgt agtaactatt ttaatgaact ggagaggaca
    15241 atggctcaag aacgttctgt aattaaattc atcagacctt gcctctccat tcatgaatat
    15301 aaaaatactt acatctgagt attgttggct gtgtttgaat aaaaagagag ctgttcaaat
    15361 aagcctgtat aactgttctt tacaaaaaat ggaggagcta tcagctaatt caacactgga
    15421 agatgttcgt cgagttggat tgaacgccct gcgcagacca ggcatttcgg attaagtgct
    15481 cgcacttcca cagtcctgga acaagtctgg ctggtagctg taaacatact ttgtacatac
    15541 cgctctggaa tagatccccg tgccacaggc ttcacagaac tttttcccgg gaaaatgctg
    15601 cccgcacatc acacaatgcc actccagcac gaccggtaat ggcgataaaa acatcgccat
    15661 atcctcaatg taagggtggg acttctccgg attcagcacc acacagtccg ccttctgttg
    15721 ctcactcagg gcatgtaaat catgctcaaa ccacgccccc tgagcatctg tctgcagaat
    15781 caaccggcca tggcaggaaa ggcagaaaca ctgccggata tttctgctga gcgcagagtc
    15841 ggcactgata atgtgctcac ccggcgtgat ccccagcccc gtttttatac cgttcattct
    15901 gctcccccct cctcactgaa gcgcccggtc tggcagtgtg tgcagtactg ctccccataa
    15961 taatcgtggt gacattgcct gcagtgccag ctggctttac gtactacggg taaggcatcc
    16021 gggacgaatt gctgcagacg tttaatcaac cgtatttctc tgcgttccgg cctgacataa
    16081 gggcactcat gaccgtgctc cgtcagccct tcgtcagcgt gttcaaacca gggccgttca
    16141 gtgtcgtatt gcgggtggta tctgagtgca ctgccgcaaa ggtggcaggt gtagctatcg
    16201 taaggtgcag tctgtgcggt acgggcaccg gtcagacgtc cgttgccatc aagtgcgaga
    16261 aaagattttg cgtacatagt atatgttcct ctgcgccaga cgacacgcag gcaacagccg
    16321 tcccggtacg ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat ctggactgga
    16381 gtttgggtaa agcaggctgg ttgctgttac agcactgtgg ttgtcacaca atggcccact
    16441 gacgtaaaaa acagtcagta agcagagatg gagattgggg gatgtgctgt atcggttgcc
    16501 ggaaggaatg gtaaagaatg tgtcatggtt aaatctccgt atggttcata cggaatgacc
    16561 acgccgtaaa aaatgcgcag tgccgctgaa gtggccgccg gaaccgacag caaagattag
    16621 cgccagcccg tacttgtcgt tcaggggttt acaccagagc acatatcccc ggcagggggc
    16681 tgtccagtcg tggtcgaccc cggttacttt tttcaggaag cccgctgcat ttcaggatga
    16741 tgccacattc tttctgttcc cgtttggcca ggtagctgat ggcatagcgt aaatcattca
    16801 ccgctttgtg atctgaatag tgaatgacgt ggttaatgtt taccggataa tttttattaa
    16861 accggcacca gtggtagtaa ccattaccct cggtaacccg cctccagata tctcccatca
    16921 atctggatac cagatacgaa ctcctgtggc tctggccgtt cagatagccc acaaaatgaa
    16981 tgtgaagccc tttacgttct gtatattcca tcacccaggc atacccgacc agtttctctc
    17041 ccacctcgtt aatcagttgc agcatctccg cgcacatggc gtacctgcag gcacatctgt
    17101 agctgagggt gttttttcgg taagcaaaat ccattctgaa cggtaacagt ttcgaataga
    17161 ccccgaataa tgaatccaga tgctggttaa agtgctccag gagctgggga gccatttcgt
    17221 aagcgggatt ggtgttataa gtttgcatgt ttcttcctcg tgttctgtaa cacaggaaga
    17281 agcgttgccg taatttttga agaagatgtt ctcaggtagt agggtgaacg tgtttcctcc
    17341 ggtaacgact tccacgggta tcagaaccag gcaaagaaca gcatacgtaa cagccataca
    17401 g
//
LOCUS       DAZGOB010000102        17344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGOB010000102 DAZGOB010000000
VERSION     DAZGOB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.102"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1222)
                     /locus_tag="ACHLIF_003604"
                     /pseudo
     CDS             complement(1..1222)
                     /locus_tag="ACHLIF_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            1205..1903
                     /gene="tagH"
                     /locus_tag="ACHLIF_003605"
     CDS             1205..1903
                     /gene="tagH"
                     /locus_tag="ACHLIF_003605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO9607554.1"
                     /translation="MSDPASNGGINTPFMDLPPIYASPGDRNDDVSAAEMAQRHLAVT
                     PLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLDLQQQRNSLSDKHLRPLEDNP
                     LRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVRHHEEANRAAIVESLRVLLDA
                     FSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEELASDRQQGFEMLFNEVYAQV
                     YDRVLREKQREPEA"
     gene            1900..2424
                     /gene="tssJ"
                     /locus_tag="ACHLIF_003606"
     CDS             1900..2424
                     /gene="tssJ"
                     /locus_tag="ACHLIF_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO9607555.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKKYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            2427..3757
                     /gene="tssK"
                     /locus_tag="ACHLIF_003607"
                     /pseudo
     CDS             2427..3757
                     /gene="tssK"
                     /locus_tag="ACHLIF_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            3762..4523
                     /gene="icmH"
                     /locus_tag="ACHLIF_003608"
     CDS             3762..4523
                     /gene="icmH"
                     /locus_tag="ACHLIF_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO9607556.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            4532..7327
                     /gene="tssH"
                     /locus_tag="ACHLIF_003609"
                     /gene_synonym="clpV"
     CDS             4532..7327
                     /gene="tssH"
                     /locus_tag="ACHLIF_003609"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO9607557.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESEQDNTGAEPADEAGSEQPEETAETVSPVQ
                     RLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWL
                     GDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYG
                     GRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKA
                     HPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLY
                     PVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVT
                     DEIMSRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTAD
                     VEDAQNDESVTKDETVL"
     gene            7324..8067
                     /gene="vasI"
                     /locus_tag="ACHLIF_003610"
     CDS             7324..8067
                     /gene="vasI"
                     /locus_tag="ACHLIF_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO9607558.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            8072..9484
                     /gene="tssA"
                     /locus_tag="ACHLIF_003611"
                     /pseudo
     CDS             8072..9484
                     /gene="tssA"
                     /locus_tag="ACHLIF_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            9593..13027
                     /gene="tssM"
                     /locus_tag="ACHLIF_003612"
     CDS             9593..13027
                     /gene="tssM"
                     /locus_tag="ACHLIF_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO9607559.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            13038..14487
                     /locus_tag="ACHLIF_003613"
                     /pseudo
     CDS             13038..14487
                     /locus_tag="ACHLIF_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            14414..14896
                     /locus_tag="ACHLIF_003614"
     CDS             14414..14896
                     /locus_tag="ACHLIF_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO9607560.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            14940..15854
                     /locus_tag="ACHLIF_003615"
     CDS             14940..15854
                     /locus_tag="ACHLIF_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607561.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIISSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            15864..16343
                     /gene="ykfM"
                     /locus_tag="ACHLIF_003616"
     CDS             15864..16343
                     /gene="ykfM"
                     /locus_tag="ACHLIF_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFO9607562.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKSFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(16480..17265)
                     /locus_tag="ACHLIF_003617"
     CDS             complement(16480..17265)
                     /locus_tag="ACHLIF_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607563.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT         4079 a         4689 c         4717 g         3859 t
ORIGIN      
        1 ccggtgcgag ggtgcgcacg ctctccagcc tgcccgtggc actgtagccg tattcccgcg
       61 tctgtcgcgg gccgctgatg cgcaccaggt cgccgttgtc attccacccg tagtcacggt
      121 catacaccag gctgttcagg tgctggctct gtaactggcc tgcgggggtg tatgtgctgg
      181 tcagttcata tgcggcatta ctgcctgccc tgctgccgaa gctgcgcacc gtctcacggt
      241 gcagcctgtc ccgcgtatac tcgaccagcg gcgtcccgcc cagcttcatg cccgccaggt
      301 aaccgctgcc gtaggtcagc cactccaccg gcggcaggct gtccggcgtg acgcggtttg
      361 ccagcccctg ttcgttgtag gcgtgtttcg tctcatgctg ccacagcagt tcccccgtct
      421 ccgggttctc caccgtctgc cgttcgcccg tcaggcggcc cttatcgtcg tacccgtagt
      481 ggaccgacac gcggtggcct tcactggtat ggctgagggt ggttaaccac ccgtgctcat
      541 catactgcca ctgctctgcc gggtcgccgt tcaccgtccg gtgcgtgatg cggtccgacg
      601 catcgtagtg ccagagggtg ataagcccct cgtcttcact ctgtgtgagt tttctggtca
      661 ggtcatagtg gtaacgttgc gtccggccgt caaaaccgcg ctgttcagtc agcctgtcaa
      721 ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcacg gtgatgcgcc
      781 cggcagcgtc ataccccatg ctgcgcgtca gaccgccttc cgtcacactc accggacgcc
      841 cgcgtttatc atatgcgatg gtgctgcgtt taccgtccgg tgataccgtt gctgtcaggt
      901 ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccttc tgactgacca
      961 gttgcccacg cgggttataa ctgctgtaag tgctgatgcc ttcctcccgg tgaacggcga
     1021 tttgttgacc gtaccggtcg tactcatacc gcgtcgtgta ccccgagcag tccgtaaagg
     1081 ccagcagttg accgtaacgg ctccatgcca tctgttttgt actgcccgtc gcgtcctgta
     1141 tccctgtcgg cagctcactc gccggccgct ttcaggcatt gcggctgtcc ggccatcggc
     1201 accgctgtcc gatccggcaa gtaatggggg gatcaatact ccgtttatgg atctgccgcc
     1261 catttatgcc agccccggcg atcgtaatga cgacgtctct gcggcagaaa tggcgcaacg
     1321 ccaccttgcg gtcaccccct tactgcgcgg tcttggcggc tcgcttacca tgagcaattc
     1381 cgacgatgcg gatgattttc tggaggaggc cggacgaacg ttacaggccg caataaaagg
     1441 tctgctcgat ttgcagcagc agcgtaacag cctctcagac aaacatttgc gcccgctgga
     1501 agataacccg ctgcgcctga acatggatta cgccaccgcg ctcgacgtaa tgtttgccga
     1561 aggtaaaagc ccggtacatc tggcggctcc cgccgccgtc agtgaaagcc tgcgcaatgt
     1621 ccgccaccac gaagaagcta accgggcagc gattgtggag tcgcttcgtg tcctgctgga
     1681 tgctttctca ccacaaaatc tgctgcgccg ctttgtgcag taccgccgca gccatgaact
     1741 gcgccagccg ctggatgatg ccggagcatg gcaaatgtac agccattatt acgaagaact
     1801 ggcctccgat cgccagcagg ggtttgagat gctgtttaac gaggtctacg cccaagtcta
     1861 tgaccgggtg cttcgtgaaa aacagcggga gccggaagca tgaacaggaa ggcgtttctg
     1921 gcctgcgtgc tgatgtgcgt catattaacc ggctgcgaaa cggcgaaaaa aatcagtgag
     1981 gtgatcaaaa acccggatat ccaggtcggg agcctgaagg agcaaccttc ggaaataacc
     2041 gtcaccctgc tgaccgaacc ggacaccaat accaatgcgg aaggtgaatc agccgccgtg
     2101 gatgtgcaac tggtgtacct gactgacgac tcaaaattgc tggcagccga ctatgaccag
     2161 atagccagta cgccgctacc ggatgtgctg ggtaaaaaat atatcgacca ccaggatttc
     2221 aacctgttgc cggacaccat caaaaccctg ccgccggtaa agctggatga gaaaacgcag
     2281 tttattggtg tcgtggccta tttctccgat gaccaggcca ctgaatggaa acaaatcgag
     2341 acggtggaag gaaccggcca ccattaccgc ctgctggtgc atgtacgtca aagcagcatc
     2401 gaaatgaaaa aagaggatga ataattatgg caaccacccg caacaaggtg atgtggcagg
     2461 aagggatgct gatgcgtcca caccatttcc agcagcagca gcgttacaac gactacctgg
     2521 ataaccagcg tttccgggcc atgaatgatt tatcctgggg atttaccgaa ctcactctca
     2581 acaatgaact gctggcacag ggtaagatca tgattgacag cgcgtcaggc acactgcccg
     2641 acggcaccgt cttttctatc cccgaccagg acgcactgcc cgatccgctg cacccgcaac
     2701 attttccgga cgagagaagc cgcaatatct acctcgctct gccggtcgcc agtgatgtga
     2761 gaaatgaaat cagcgacggg cggcgaatcg ggcgttaccg gctgaattat gccgatgtcc
     2821 gggatttgca ttcagaagaa ggtgacacgc gaacactgac gctgggacaa ctgacgccgc
     2881 gcatatgagc ggtgcagaag atatgagcgc ctatattacg ctcccgcttt gccgtatcag
     2941 cgatcgccat gccgatggct ccctgacgtt ggatgacgat tttatcccct cctgccagaa
     3001 tattcaggtc agtaagaaac tgcgtgttta tctcaaagag gtacaggggg ccattggcgg
     3061 acgggcaagc gatctggcaa accgtattgg ctctccggcg cagagcggca tcgcggatgt
     3121 ggcggaattt atgatgttgc agttacttaa ccgtaaccag acccggttta cccatcgcgc
     3181 ccgccgatcc cagctccacc cggaagattt ctaccttgat cttgctgggt tgctgggtga
     3241 actgatgacc tttaccgagc cgtcgcgcct gccctgcccg cttgatgtgt atgatcatca
     3301 tgatctgacc aaaacattta aaacactgtt accggaagtc aaacgggcgc tgcataccgt
     3361 actgtcgcca agagcggtca atctgccgct gcatctgcgt gacggtatct ggcaggccga
     3421 tatccatgac acagaactgc tgcaatctgc cacctttgtg ctggccgtgg cggcaaacgt
     3481 gccggtcgat cagatccagc gtcagtttat ccagcagtcg aaaatttcct cgccggaaaa
     3541 aatccgcaat atggtcagtg tgcagatccc cggcattcca ttgcgcgcac tgatggtggc
     3601 tccccgccag cttccttacc attccgggtt cagctatttc gaactcgaca agagcggaca
     3661 ggcctggaca gaaatggctg ccgccggggc cgttgcactg catgtatccg gcagtttccc
     3721 ggatctgaac atgcaactgt gggcgataag agggtaagac gatggatgag gggtcactgt
     3781 ctctgccacc atttaccgga tacgacgaaa aaagccagcg cgactatcat ctcgcgcttc
     3841 gtggcaacag cctgaatccg atgatcgacg cagccacgcc gctcctcggt atggtgatgc
     3901 gcctttcaac catgaacagc cagacgatgc cggagcatct ttttgctcag gtggtgacgg
     3961 atgttcaggc cgtggagcaa ctgcttcagg agcagggcta cgaaccgggc gtgattatct
     4021 cgtttcgcta catcctctgc acgtttattg atgaagcggc gctcggtaac ggctggtcga
     4081 acaagaacga gtggatcaag cagtcattgc tggtccattt ccacaacgaa gcctggggtg
     4141 gagagaaagt tttcatcctg ctggagcgcc tgatccgtga acccaaacgt taccaggatc
     4201 tgctggaatt tctctggctc tgtttttctc tgggatttcg cgggcgctac aaagtggcag
     4261 cacaggatca gggggaattt gagcagatct accgccgcct gtaccacgtc ctgcataaac
     4321 tacgcggcga tgcccctttc ccgctgttac accaggacaa aaaaacccag ggcgggcgtt
     4381 atcagctcat cagtcgcctg accattaaac atattttttg tggtggcgtt gtcgtgctgg
     4441 cgttgtttta cctgttctat ctgttgcggc tggacagcca gacgcaggac atcctgcacc
     4501 agctaaacaa attacttcga taaggacgtt tatgatccag attgatcttc ccacgctggt
     4561 aaaacggctt aacctgttct cccgccaggc gctggagatg gccgcctctg aatgtatgag
     4621 ccagcaggca gcggaaatta ccgtcagcca tgtgcttatt cagatgctcg ccatgccacg
     4681 cagtgacctg cgggttatta cccgccaggg cgatattggc atggaagagt tgcgccaggc
     4741 gctgacggtg gagaactaca caactgcccg ttctgcggac agctacccgg cattttcccc
     4801 gatgctggtt gagtggctga aagagggctg gctgctggcg tcggctgaga tgcagcacag
     4861 cgaattgcgt ggcggcgtgt tgctgctggc cctgctgcat tcgccgctgc gttatatacc
     4921 gcctgctgcc gcccggctgt tgaccggcat taaccgtgac cgtctgcaac aggactttgt
     4981 gcaatggaca caggagtcgg cggaatcagt cgtgccggat gcagacggta aaggcgcagg
     5041 cacactgacg gacgctgctg acaccctgct tgcccgctac gccaaaaaca tgaccgcaga
     5101 cgcccgtaac ggcaggcttg acccggtact gtgccgcgac cacgaaatcg acctgatgat
     5161 cgacattctc tgccgccgcc gcaagaacaa cccggtggtg gtgggcgaag caggcgtggg
     5221 taaaagcgcc ttaatcgaag ggctggcgct gcgcatcgtg gcaggccatg tgccggacaa
     5281 gctgaaaaac accgatatca tgacccttga cttgggcgca ttgcaggccg gagcgtcggt
     5341 gaagggtgaa ttcgaaaaac gtttcaaagg gctgatggca gaggtcattt cctccccggt
     5401 gccggtcatt ctgtttatcg acgaagcaca taccctgatt ggcgcgggca accagcaggg
     5461 cgggctggat atctccaacc tgctcaaacc ggcgctggcg cgcggcgagc tgaaaaccat
     5521 cgccgccacc acctggagcg agtacaaaaa atacttcgaa aaagatgccg ccctgtcgcg
     5581 ccgcttccag ttggtgaagg tcagcgaacc caacgctgcc gaagccacca ttattctgcg
     5641 cggtctgtcg gcggtctatg aacagtctca cggcgtgctg attgatgatg acgccttgca
     5701 ggccgctgcg acattaagcg agcgttatct ctccgggcgt cagttaccgg acaaagcgat
     5761 tgatgtgctg gataccgcct gcgcccgtgt ggccatcaac ctgtcgtcgc cgccgaagca
     5821 aatctcggcg ctgaccactc tgagccacca gcaggaggcg gaaattcgcc agcttgagcg
     5881 cgagcttcgc atcggactgc gtaccgacac atcacggatg accgaggtgc tggtgcagta
     5941 tgatgaaacg ctgacggcgc tggatgaact ggaagcggcc tggcaccagc agcagacgct
     6001 ggtccgggag attattgcgc tgcgccagca gttactgggc gtggcagagg acgatgcggc
     6061 gccgttgccg gacgcagata ccgtggagga tacgcagcca gagtcagagt cagagtcaga
     6121 gtcagagtca gagtcagagt cagagcagga taataccggt gccgaaccgg ctgatgaagc
     6181 tggcagcgaa cagccggaag agaccgctga aacagtttcc ccggtacagc ggctggccca
     6241 gctcactgcc gaactggacg ccctgcataa cgaccggttg ctggtctccc cgcacgtcga
     6301 taaaaaacag attgcggcgg tgattgccga atggaccggc gtgccgctca accgcctatc
     6361 gcagaatgaa atgtcggtca tcaccgacct gacgaaatgg ctgggtgaca ccatcaaagg
     6421 ccaggacctg gcgattgcca gcctgcataa gcatctactg accgcacgcg ccgacctgcg
     6481 tcgtccggga cgcccgctcg gtgcgttcct gctggctggc cccagcggtg tgggtaaaac
     6541 cgaaaccgtc ctgcaactgg cagagctgct ctacggcggt cgccagtacc tgaccaccat
     6601 caatatgtcc gaattccagg agaaacacac cgtctcgcgg ctgattggtt cgcctccggg
     6661 ctacgttggc tacggtgaag gcggcgtact gaccgaagcg attcgccaga aaccctactc
     6721 ggtagtactg ctcgatgaag tggaaaaagc gcacccggat gtgctcaacc tgttctacca
     6781 ggcgttcgat aagggcgaaa tggcagacgg tgaaggccgc ctgattgact gcaaaaatat
     6841 cgtcttcttc ctgacgtcca acctcggcta ccaggtaata gtcgagcatg ccgatgaccc
     6901 ggaaaccatg caggaagtac tgtatccggt gctggccgac ttcttcaaac ctgccctgct
     6961 ggcgcgtatg gaagtggtgc cgtacctgcc gctgtcgaaa gagacgctcg ccaccatcat
     7021 tgccgggaaa ctggcccgcc tggataacgt gctgcgcagc cgctttggcg cggaggtgat
     7081 tatagaaccg gaagtgacgg acgaaatcat gagccgcgtc acccgcgcgg aaaacggcgc
     7141 gaggatgctg gaatcggtca tcgacggcaa tatgctgccg ccgctctccc tgctgctgtt
     7201 gcagaaaatg gcggcgaata cggcggttgc ccggattcgg ttgtcggcag tggacggcgc
     7261 atttacggca gacgtggaag atgctcagaa cgacgagtcc gtcacaaagg atgaaacggt
     7321 tttatgagca ggttgcgcgt tttccggcag acaggatggt ttatcgcggg cctgatgacg
     7381 ggcttacccg ccactgccgc gcccgcagaa gcatcgtcaa tggccggtgt ggcggtcgcc
     7441 gtcgccacca cgaccccgcc ggatgcgaca gccacgttac aggccatgca gtcctgtcgg
     7501 cgggagtccg cggcgctgga gcggcttgac tgctatgacc gcctgctggc accgctgtcc
     7561 ccgtcagggt ttgacggggc gctggtcaaa gccggttttg tgggcgaggc ctggacccgc
     7621 gccacagagc aggaaaaacg ccgcgagggc aacaccacag aactgctggt gacgcaggtg
     7681 ccgggggagc gcccgacggt ggtgataacc accccggcta tcggtcatgt tccgccacgc
     7741 ccggtgctga tgttcagttg cgtggacaat atcacccgga tgcaggtggc gctgatgcac
     7801 cctctggatg tccatgatat cgccgtcact ctgaatgccg acaaccgggc attgcgcagc
     7861 cactggtttg tgcgcgagaa cggcaccctg ctggagtcca gccgggggtt atccgggatt
     7921 gatgaaatta agcaactgtt cggcgcaaaa acgctgacgg tggataccgg cgcagacaat
     7981 gccgccggaa agctgacttt taacattgac gggctggcaa gggcgattgc tcccctgcgc
     8041 gatgcctgcc actgggcggg agaataaaaa tatggcaatg gacttacgcg atccgaatgt
     8101 ctggatatcg cacctgctgg aaaacctgcc ggaagagaaa ctggcatcgg cgctgaaaga
     8161 tgacaacccg aactgggagt atatcgacgg cgaaatcgtc aagctgggat cgctggccca
     8221 cagtcaactg gatatcccgg aactccagcg ccgtgggctg gtcattctgg cttcggagag
     8281 taaggatttt cgtctgctgg cgcatctgct gagaaccctg caacacgccg gtgatcctca
     8341 tctcgcattg cgcctgctgg cgctgtatgt ggagcattac tgggcggtgg ctgcgccgca
     8401 gaatatggcg cataaaaaac gctttgccag ccaggtcatt aaacgctttg aaacaggtat
     8461 tgaagttttt tcacagaacg ccgccacagc gcagcgcgat gcgctgtcag gggaactggc
     8521 aaaactggcg caatgctggc agtcacataa cgccccggaa ctggcacagg ctactgatga
     8581 tctttttgcc ctgtaccagc gtgcgtttaa tcgtgcggca ccggctcctg tccccactcc
     8641 ggcggcctcc ggtagttcac cacagaccac cgccacgtct gaaagcggcg tgacgcaacc
     8701 cagtgctccg gctccccaaa tagtcatcga cagtcacgac gacaaagcct agcgcgacac
     8761 gctgttaaaa gtggcggcca ttttatgtga acgccagccg gattcaccgc agggctaccg
     8821 tctgcgccgc catgcgcttt ggcagaacat caccagtacg ccccaggcgg aaagcgatgg
     8881 ccgtactcca ctggctgcgg tctccgccga tatggtggca gattaccatg ctcagctcgg
     8941 tagtgccgat atggcgctgt ggcagcaggt ggagaaaagc gtactgctgg ctccttactg
     9001 gctggatggt cactgccttt ctgcacagac ggcgctgcgt ctgggttaca aacaggtggc
     9061 agacgccatc cgcgatgagg tcatccgctt ccttgagcgt ctgccccagc ttaccgggct
     9121 gctgtttaat gaccacacac cgtttatcag tgagcagacg aaacaatggc tggcagcttc
     9181 gcccgacgcc aaagttgcac cggttgcgca aatcggtgag gaatcgaagg cagccagagc
     9241 ctgttttgct gagcagggtc tggaggcggc gctgcgatat ctggacatgc tacccgaagg
     9301 cgatccccgc gatcagtttc accgccagta ccttgccgca cagttgacgg aagaggcggg
     9361 gctggtacag cttgcgcagc agcagtaccg gatgttgttc aggatgggat tgcagatgat
     9421 ggtggcagac tgggagccgt ccctgcttga gcagctagaa caaaaattca cggcagaaca
     9481 ataaaagaga acaggagttt ttgtgttcag attaccaaca ccccgactac tcagcggact
     9541 caaatcagcc ctgcgaccgg cgatgccccg gtttaaagtc tctgctttct ggctgctgat
     9601 actggcgtgg atttttctgc tggtgtggat ctggtggaaa ggcccgatgt ggacgctgta
     9661 tgaagaacag tggctcaaac cactggcgaa ccgctggctg gcaacggcgg cgtgggggat
     9721 tattgccctg gtgtggctta ccgtccgggt gatgaagcgc ctgcaacagc ttgaaaaaat
     9781 gcaaaagcaa cagcgcgagg aagccgttga tccgctcagc gtggaactga acgcccagca
     9841 gcgttatctt gaccgctggc tgctgcgcct gcaacgccat ctcgacaacc gccgtttcct
     9901 gtggcagttg ccgtggtata tggtcatcgg cccggcgggc agtggcaaaa cgacactgct
     9961 gcgcgagggg tttccgtccg acattattta tgccccggag ggcgcacgcg gcgcagaaca
    10021 acgcctgtac ctcacgcccc atgtcggtaa acaggcggtg atctttgata tcgacggcac
    10081 actctgcgct cccgctgatg cggatatcct gcatcgccgt ctgtgggaac atgccctcgg
    10141 ctggctgaag gaaaagcgcg cacgccagcc gctgaacggg attattctga cactcgattt
    10201 acccgatctg ctgaccgcag acaaacgccg ccgcgagcat ctgttacaga cgctgcgcag
    10261 ccgcttacag gatatacgcc agcatcttca ctgccagtta ccggtttacg tggtactgac
    10321 ccggcttgat ttattacagg gttttgccgc cctgttccag tcactcaaca gacaggatcg
    10381 cgatgcgatt ctgggcgtca cgttcacccg ccgcgcccat gaaaatgatg actggcgaac
    10441 agagttgaat gctttctggc agacatgggt ggatcgaatg aatctggcgt tgccggatct
    10501 gatggtcgct cagactcaca cccgcgcgtc tttattcagt ttttcccgcc agatgcaggg
    10561 aagccgtgaa ccgctggtgt cactgcttga gggtctgctt gatggcgaaa atatgaacgt
    10621 gatgctgcgt ggtgtctatc tcacctcttc gcttcagcgt gggcagatgg atgatatatt
    10681 cacccagtct gccgcccgcc agtaccggct gggcaataac ccactggcgt cctggcccct
    10741 ggtggacacc gcgccttatt tcacccgcag cctgttcccg caggcattac tcgcagagcc
    10801 taatctggca acagagagcc gcgcctggct gatacgttcc cgtcgccgcc tgacggtttt
    10861 ctctgccaca ggcggcgtgg cagcactgct gctcatcacc ggctggcatc actattacaa
    10921 cggtaactat cagtccggca tcaccgtgct taagcaggcc aaagccttta tggacgtgcc
    10981 gcctccgcag ggggaagatg actttggcaa cctgcaactg ccgctcctga acccggtacg
    11041 cgatgccaca ctggcctatg gcgactgggg cgaccgcagc cgtctggccg atatgggact
    11101 gtaccaggga cgacgtatcg ggccttatgt ggaacagacc tatctgcaac tgctggagca
    11161 acgttacctg ccctcgctgt ttaacgggct ggtcaaagcg atgaacgccg cgccgccgga
    11221 gagtgaagaa aaactcgcgg tgctgcgcgt aatgcgaatg ctggaggaca aaagcggacg
    11281 taacaatgag gtggtgaagc agtatatggc aaaacgctgg agcgaaaaat tccacggcca
    11341 gcgcgatatc caggcacaac tgatgtccca tcttgactac gcgctggctc atactgactg
    11401 gcatgcagag cgtcaggcgg gcgacggtga cgccatcagc cgctggacgc catatgacaa
    11461 gcccgtggta tcagcacaga aagaactgag caaactgcct gtctaccagc gggtttacca
    11521 gagcctgaaa acgcgggcgc tgggcgttct tcctgccgac ctcaatctgc gtgaccaggt
    11581 agggccaacc tttgaccagg tgtttacatc tgccgatgac aacaaactgg ttgttccaca
    11641 gtttatcacc cgctacggcc tgcaaagcta ttttgtaaaa cagcgcgatg aactggttga
    11701 actgacagcg atggattcct gggtacttaa cctcacccgc aatgtgaaat acagtgacgc
    11761 cgaccgtgcg gaaatccagc accagttgac cgagcagtat atcagcgact acaccgccac
    11821 ctggcgggct gggatggaca atctgaatat ccgcaatttt gagtccatcg gacaactgac
    11881 cggggcgctg gagcaggtta tcagcggcga ccagcctttg cagcgggcgc tgaccgtgct
    11941 gcgtgacaac acacagccag gcgtcttttc tgaaaaactc tctgccaaag aacgggagga
    12001 agccctggca gagccggatt accagttact cacccgcctc gggcatgaat tcgccccgga
    12061 aaacagtacc ctggcagtac agaaagacaa agaaagcacg atgcaggccg tgtatcagca
    12121 actcaccgag ttgcaccgct acctgctggc aatccagaac gcgcctgtac cagggaaatc
    12181 ggcgctgaaa gccgtgcagt tacggcttga tcagaacagc agcgatccga tattcgccac
    12241 ccgccagatg gcaaaaacgc tgcctgctcc gctcaaccgc tgggttggca ggctggctga
    12301 ccaggcctgg catgtggtga tggtggaggc tgttcattat atggaagtgg actggcgcga
    12361 cagcgtggtg aaaccgttta acgagcaact ggcaaataac tatccgttta atccgcgttc
    12421 tgcacaggat gcctcactgg atgccttcga acgcttcttt aaaccggatg gcatactgga
    12481 taccttctac cagcagaacc tgaagctgtt tatcgataat gacctgagtc tggaggatgg
    12541 cgataacaac gtcattattc gcgaagatat tattgcgcaa ctggaaactg cgcagaaaat
    12601 ccgtgacatc ttcttcagca aacagaacgg tctgggaaca tcctttgccg tggaaacggt
    12661 atcgctttca ggcaataaac gccgcagtgt actgaacctt gacggtcagt tagtcgatta
    12721 cagccagggc cgtaactata ccgcccatct ggtctggcct aacaacatgc gcgaaggcaa
    12781 cgaaagtaag ctgacgctca tcggcaccag cggcaacgcg ccgcgcagta tcagcttcag
    12841 cgggccgtgg gcgcagttcc gcctgttcgg ggccggacaa ctgaccggag tacaggatgg
    12901 caactttacc gtgcgcttta gcgtggacgg tggcgcgatg acctaccgtg tgcataccga
    12961 cacggaagat aacccgttca gcggtgggtt gttcagccag tttggtctgt cagacacact
    13021 gtactgagga ggcagggatg aatagtaacg tactgacaca aactatcgtt accggcagtg
    13081 acccgcgcgg gctgccggaa ttcagcgcca tccgcgagga aataaacaaa gccagccacc
    13141 cgtcacagcc tgagctgaac tggaaactgg tggagtcgct ggcgctggcg atttttaaag
    13201 ccaacggtgt ggatttacac accgccacct actatacgct tgcccggaca cggacacagg
    13261 gactggcggg attctgcgaa ggtgcggaac tgctggcggc aatggtaagc cacgactggg
    13321 ataagttctg gccgcagggc ggcccggcgc gtactgaaat gctggactgg tttaactccc
    13381 gaaccggcaa tattctgcgt cagcaaatct cctttgcgga atccgaccta ccactgatct
    13441 accgcacaga gcgggcattg cagcttatct gcgataagct ccagcaggtg gaactgaagc
    13501 gcgttccgcg cgtggagaat ctgctctatt ttatgcagaa cacgcgtaaa cggcttggac
    13561 cccagctgaa gagtaacact gagaacgccg cacagaccac ggtcagaacg ctgatttatg
    13621 ccccggaaac acaggcatct tccacaccag aagcggtagt gcctcccctg cccggcctgc
    13681 ctgagatgaa agtggaagtt cgcagtctga cagagaatcc cccacaggcc agtgtgataa
    13741 atcaaggcag tacggtaaga gggtttatcg cagggatcgc ctgttcagtg gctgtcgcct
    13801 cagcattgtg gtggtggcag gtctatccgg tgcagcagca actgttacag gttaacgaca
    13861 ccgctcaggg cgcagcaacg gtgtggatgg cctcacctga actcgaaaac tatgagcgca
    13921 ggctgcaaca acttcttgat acctccccgg tacagccgct ggaaaccggg atgcagatga
    13981 tgcgtgttgc cgacagtcgc tggccggaaa gcctgcaaca gcaacaggcc tcgacacaat
    14041 ggaatgaggc actcaaaacc cgcgcacaga gtagcccgca gttgcgtggc tggttgcaga
    14101 cccgccagga cttacatgct tttgcagatc tagtgatgca gcgcgagaaa gagggactaa
    14161 ccctttccta tatcaaaaat gtcatctggc aggcggagcg gggactgggg caggaaacac
    14221 ccgttgagtc tctgttgacg cagtaccatg atgcccgtgc gcagaagcag aatacagatg
    14281 cgctggaaaa acaaattaat gagcgactcg aaggcgtgtt aagccgctgg ctgctgctga
    14341 agaataacgt catgccagag gcggcaaccg gtaccacggc cgaaaaataa ttatttaatt
    14401 aaaggagatt atcatggcaa atataagtta tttatctttg agtggagaga cacaagggct
    14461 aatttcagca ggttgctcaa cgcttgattc tgtcggaaac aaagcgcagc cagaacataa
    14521 agatcagatc atggtttatg cgttaatgca ttccatctca agaagtcaaa atgtcaatca
    14581 tcatgaatta attatcacca aaccggttga taaatcctca ccattgctgg caaaagcact
    14641 tagtgacaat gaaaaaatgg caatatgtga atttatactc taccgaacca gtaaagcggg
    14701 tatttaccag ccttattata aaataaatct gtcaaaagcc cgaatatcaa gtattgattt
    14761 tgtcacaccg catgctgtcc tggaaaaaga actggaacca caggagagaa ttgcctttat
    14821 ttatgaagat atttcctggg agcatacgtt agccggtacg aatgcaatga gtaaatggca
    14881 ggatcgtgta cagtaactta ctgtaataac ataataaatt actattgcgg agagataaaa
    14941 tggctggttt aaggaataac aacaacacac agaatgcaca gtgggctgat tatgtgggtg
    15001 acattcttcg gggggctcag ccgataaacc agctagtacc tcagcaccct tatcttaacg
    15061 acgtcccact cattgatgag ttaaggcatc agaacactca tcatgttatt ctcttgacac
    15121 ttgatgttgc aaaaaaaata ctcagcccaa taacatcatt tgattacatt cattttatca
    15181 ctacacatcc ttcaggtata aaagatacac tcgcctggtt agttaacgcg ggtaaattaa
    15241 tgactgagtt tgatgacaat gggaaaatta tatttaacct taatgcctta aagtatacaa
    15301 aagcaagtta ctttgaaata cttggagaga aatatataaa aatcacaaca tccagcccct
    15361 ggcttttaga aaagctcggt aaatatattt tctcttcacg agcgcctcaa gttctggaac
    15421 tcgctattgg ctggcgagga gctttatctg aatcaataaa aggagttaag ttctgtatct
    15481 ggttttcagt agcctggaga accatagaat ttattataag ttcagagcga gatctggtta
    15541 attttcttgg cgatttttca atggatgtgg ccaaagcagt tatagccggt ggtgttgcaa
    15601 ccgctattgg aagcctggct tcttttgcct gtgttagctt tggctttcca gtaattcttg
    15661 tcggaggagc aattttactg acagggatag tgtgtacagt tgttttaaat gaaatcgatg
    15721 ctcaatgcca tttatcagaa aaattaaaat atgcaattag agatggacta aaacggcaac
    15781 aggaacttga taaatggaaa agggaaaaca tgactccatt tatgtatgtt cttaacactc
    15841 cacccgtgat atgaggatta accatgcgtt tacatgttaa attaaaagag tttctttcga
    15901 tgttttttat ggccatatta tttttccctg cattcaatgc ttctttgttt tttaccggag
    15961 ttaaacctct atattcaata ataaaatgct ccacggaaat attttatgat tggagaatgt
    16021 tgattctgtg ctttggtttc atgtcgtttt cctttttaaa tattcatgta attttactta
    16081 cgataataaa atcattctta atcaaaaaaa cgaaggtggt caatttcgcc acagacataa
    16141 caatacagct aactcttata tttttactaa tagctatcgt aatagcacct ttaatagctc
    16201 cttttgtcac aggatatgtc aatgctaatt atcacccctg cggtaataat acggggattt
    16261 tcccaggagc aatctatatt aaaaatggca tgaaatgtaa taatgaatat atttctcgca
    16321 aggaagattc agcagttaaa taattaaata ttctaaaaca aacagttttt aactccggat
    16381 tccatgtcct ttcttgcact gtctttgtca agaaaaattt cttatgcaaa gaattttgta
    16441 ttattgccgc cagaaattcc tggcggcaga tataatttat tattgatagc ggttaaccaa
    16501 atcttttacc atcgctttat aggtcttttg cttaagttgc atgatttgct gctctgtcat
    16561 atcagtcgta ctggcttcat tcttgtcggg caatggtgga agcactttat attcataatt
    16621 caccggcgac caggtagccc actcacctgt tgccacatcc accagcgtga aacgtaccag
    16681 gtagcgcagg gagatagcgt cggtaagttt ttcattttta tagtcggacc atactgttga
    16741 tttcagcgca gagtcatatt ttcctgtctg caacgtatcc tggaaaacaa taatcgcttt
    16801 ctgatgccct tttgcagcca caaaacgcag tgcctgcatc cagttcatat tctcagcact
    16861 ggcaacatcc tcgttctcat ttttatcttt gtttttgtta caagtcagag gcttttgcct
    16921 gtccgggata ccagagaatg tggaaacagt ataatatttt cgcatctcct cctgcataat
    16981 ggtttccggt gcgcggttgc cggattgaac aagtattaca ggagaattaa ggggaacgcg
    17041 gaactcatct ccatcaagcg cagcttgaat atcctcttcg gatacggcca gagtggtttc
    17101 attaccgaat aaatctttgt ctgtcagttg ctggggagtg tttttattaa tattactttg
    17161 tgtgacttga gcttttttct tcttttcatc aatagaagat tcagaggcac atcctgcaag
    17221 cagcgagaat aacacacata tgcaacaaag cttttttgaa ttcataattg gacactccct
    17281 cgcctgtcat aatcatggta atgtttagct aacgattttt aattatatac tccacttgaa
    17341 tcag
//
LOCUS       DAZGOB010000103        17342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGOB010000103 DAZGOB010000000
VERSION     DAZGOB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.103"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(173..1763)
                     /gene="lsrK"
                     /locus_tag="ACHLIF_003618"
                     /pseudo
     CDS             complement(173..1763)
                     /gene="lsrK"
                     /locus_tag="ACHLIF_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
     gene            complement(1842..2795)
                     /gene="lsrR"
                     /locus_tag="ACHLIF_003619"
     CDS             complement(1842..2795)
                     /gene="lsrR"
                     /locus_tag="ACHLIF_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO9607564.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3044..4579
                     /gene="lsrA"
                     /locus_tag="ACHLIF_003620"
     CDS             3044..4579
                     /gene="lsrA"
                     /locus_tag="ACHLIF_003620"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO9607565.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLEALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASPKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETFYGLRTLRSGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIS
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4573..5601
                     /gene="lsrC"
                     /locus_tag="ACHLIF_003621"
     CDS             4573..5601
                     /gene="lsrC"
                     /locus_tag="ACHLIF_003621"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO9607566.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5601..6593
                     /gene="lsrD"
                     /locus_tag="ACHLIF_003622"
     CDS             5601..6593
                     /gene="lsrD"
                     /locus_tag="ACHLIF_003622"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO9607567.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6605..7627
                     /gene="lsrB"
                     /locus_tag="ACHLIF_003623"
     CDS             6605..7627
                     /gene="lsrB"
                     /locus_tag="ACHLIF_003623"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO9607568.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7654..8490
                     /gene="lsrF"
                     /locus_tag="ACHLIF_003624"
                     /pseudo
     CDS             7654..8490
                     /gene="lsrF"
                     /locus_tag="ACHLIF_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462959.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
     gene            8489..8983
                     /locus_tag="ACHLIF_003625"
                     /pseudo
     CDS             8489..8983
                     /locus_tag="ACHLIF_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(8987..9901)
                     /gene="yneE"
                     /locus_tag="ACHLIF_003626"
     CDS             complement(8987..9901)
                     /gene="yneE"
                     /locus_tag="ACHLIF_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO9607569.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDIQFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10108..11559)
                     /gene="uxaB"
                     /locus_tag="ACHLIF_003627"
     CDS             complement(10108..11559)
                     /gene="uxaB"
                     /locus_tag="ACHLIF_003627"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO9607570.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLARQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(11786..13204)
                     /locus_tag="ACHLIF_003628"
     CDS             complement(11786..13204)
                     /locus_tag="ACHLIF_003628"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9607571.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13343..13702)
                     /gene="yneG"
                     /locus_tag="ACHLIF_003629"
     CDS             complement(13343..13702)
                     /gene="yneG"
                     /locus_tag="ACHLIF_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO9607572.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(13702..14628)
                     /gene="glsB"
                     /locus_tag="ACHLIF_003630"
     CDS             complement(13702..14628)
                     /gene="glsB"
                     /locus_tag="ACHLIF_003630"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO9607573.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(14692..16080)
                     /gene="sad"
                     /locus_tag="ACHLIF_003631"
     CDS             complement(14692..16080)
                     /gene="sad"
                     /locus_tag="ACHLIF_003631"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO9607574.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16181..17062
                     /gene="yneJ"
                     /locus_tag="ACHLIF_003632"
     CDS             16181..17062
                     /gene="yneJ"
                     /locus_tag="ACHLIF_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9607575.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17140..>17342
                     /gene="yneK"
                     /locus_tag="ACHLIF_003633"
     CDS             17140..>17342
                     /gene="yneK"
                     /locus_tag="ACHLIF_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO9607576.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLS"
BASE COUNT         4354 a         4449 c         4306 g         4233 t
ORIGIN      
        1 tatgcttaat gctttaagaa aaatagcaat tttcccttga atatagtact ggtgatggaa
       61 cgatgaatct gcaagctggc atttaacaag ccagctctaa aagaagggaa ataagaaaaa
      121 ctatactcaa aaactaacag ccacggtcat catgatgtgg ccgtcaatga aactataacc
      181 caggcgcttt ccataacgac gtcgtcagtc catgatcaac cagccccagc tgatcctgat
      241 aaactgcctg ccacttatcg cgtgaatcct gataaagttc atgcttttcc gggtctggtg
      301 tgtgcgtccg ttcccagcga accaggcgtt ctccggtttc tgccattgat gaaaaaattc
      361 cggcaccgac gccagctgca atggcacatc ctaatgcggt ggcttctttg actaccggaa
      421 tattgacggg taatcccgag acatcagcga gaatttgact ccataatttc ccttttgaac
      481 ctccgccagc aaaaaccagc gaggtgggat gaatattcga gaaatcagca atttgctgca
      541 agttacacgc tgatacaatc gccgcatttt cttccagcgc acggaacaat gtcgctttgt
      601 tacatttatc cgggtcaatg gacaagttaa taaaggaagg cgcagcgtga taccaggttt
      661 taaagcgcat tctgtcggag aagatcggca ttacgcccca cgacccaggc ggcacccgac
      721 tggccatctc ttccagcagc gtataggtgt cgatgcctaa acgttcggca atcagttttt
      781 cttcggcaca gaaagcatcg cggaaccagc gcatggtgag tccggtaaaa aagcttatag
      841 attcagcttg taccatgcca ggaataacat gaggattaac gcgcacgttc atttctgggt
      901 ctgtcaccgg cgcggctaaa tttacaactt gctgccagaa tgtgccgcca agaaccgcgg
      961 tttgtgccgg acgcacaacg cctaacccaa ggcaaccaag ctgcacgtcg cctcctccaa
     1021 cgaccaccgg agtgcccgcc ttcagaccgc agagttccgc cgcttgtgaa cttaccacgc
     1081 ccagcaatgt gccggtttct ttgacaggag aaagaatatc ggcacgcagg ccagccatat
     1141 ccagcaatgc aggtttccag tcacgggtgg ttagatcaaa gtcccgtggt gccagcgtta
     1201 gagggatcca ccgccagttc gccgctgagc atataggcca gccagtcgct gatcatggtg
     1261 atggttgatg cctgacggta aatatcggaa cgatggtgcg ccagccaaag taatctgggg
     1321 atggcactta aagccagtgt ttgtccggtc gcgcgataaa cttcgttttc aaaggtattg
     1381 ttgtgcagtt ctttaagttc gctaacttcg cgcgctgctc tggcatccac attggcgcag
     1441 gcccagatcg gtgttccttc attgttatat aaaacaatgc cttcacgcat agaacatgcc
     1501 gaaacggcag cgatatactc cgggtctatg ccggcgttgt gcagcgcctg gcgcatacac
     1561 tcacacgcca gttgccagtt tttgttgaga tcaaattcca tagaaccagg aacgtccggt
     1621 actgccagat gccgccactc cgcctgtccc actgctattt gattgccttt caggtcgaaa
     1681 atcacagccc gaatacttcc ggtgcctgca tccagcgcca acaggtaata ctttgattct
     1741 gaaggggtaa agagtcgagc cattagatta tcctcggctt atgcgttctg tctgttcctc
     1801 tatacgttct ccatcattcc cggtaataag gtcatgcaaa tttaactacg taaaatcgcc
     1861 gctgctgtat cctgatcggt aaccagtgcg ttgatataac cgcctttcat tgcagcggca
     1921 attgcttcgg ctttattttc tccccctgcc acgccaaccc ggacgggtat ggtcttcagc
     1981 gcgcttaaag gtaagccaat cagttcgtta tgtattttaa tatccgtgac aacgtcacct
     2041 tttgcatcaa aaaagtagcc taaaatgtcg ccaaccgccc cttttcgacc aatcattaac
     2101 tgttcgccct ggctgatata accggagcga atgatcgtcg cgtcgtcctg ttgactgacg
     2161 gccccaatgc cgacaatcgc cacatccgct gcttgcgcgg ctaacagaac atctttgacg
     2221 caattttcat tttttagcgt acgggcaatg tcagcggagg atgcccgcaa cggagccgga
     2281 ataatattca cactgcacgc cgcgttaagc tgcccgatac ccgtcatata agaaccgacg
     2341 ccaccggaga gcgtgaccag gcgaatttgc tgtgacgaaa taaaaccact taagcgttgc
     2401 agcgtattca tggttgcctc gccaaaacca atcgccagca tctgttgtgg ttgaagtaaa
     2461 ctcatcaaca tatgcgccgc gcctatccct agtcgcccgc cgacatcagc atccgcaagc
     2521 ccagggatca cccggacatg ttgcagcgaa aactgacgac gtaattgagt ttcatattcc
     2581 agacagcctt caaagcgaga attaatctgt acgcgaataa tgccggactg atgccctttc
     2641 tccagcaagc gcgacacttt caaacgggtc aggccgagac gatcgctgat ctcgctctgg
     2701 gtcagcccgt cgtgatagta aaaccacgcg atccgcgcga cctgttcttc ttcacacatt
     2761 ccctgttctg aaattgccga atcgttgatt gtcataattc attcttcact ttgaacatat
     2821 ttaaatcttt aatgcaattg ttcagttctt gcgcatttat atctgtgatg gcagccacag
     2881 tttgactcta cgagcatgaa caaacgcaac cgtgaaaatc aaaatagcat aaattgtgat
     2941 ctattcgtcg gaaatatgtg caatgtccac ctaaggttat gaacaaatta aaagcagaaa
     3001 tacatttgtt caaaactcac ctgcaaaact gaacggggga aatatgcaaa cgagtgatac
     3061 ccgcgcgtta ccgctacttt gcgcccgctc ggtttataaa cagtattcag gggtcaatgt
     3121 cctgaaaggc atcgatttta cgttgcatca gggggaggtc cacgccctgc tcggcggcaa
     3181 tggtgccggt aaatcgacgt taatgaagat tattgccggt attacccctg ctgatagcgg
     3241 tacgctggag attgggggca acaactacgc cagattaacg ccagttcatg ctcatcagct
     3301 gggcatttat ctcgttcccc aggaaccgct gcttttccca agcctgtcga taaaagaaaa
     3361 catcctgttt gggatggcaa aaaaacagct ctccatgcaa aaaatgaaga acttgctgga
     3421 ggcgctgggc tgccagtttg atctgcatag tctggcagga tcgctggatg tcgccgatcg
     3481 ccaaatggtg gaaatcctcc gcgggctaat gcgcgactcg cggattctga tcctcgatga
     3541 acctaccgcc tcgcttaccc ctgcggaaac cgaacgcttg tttactcgct tgcaagagct
     3601 gcttgctact ggcgtgggta ttgtttttat ctcgcataag ctgccggaaa ttcgccagat
     3661 tgccgatcga attagcgtga tgcgcgacgg aaccatcgcc ttaagcggca aaaccagcga
     3721 actgtctacc gacgacatta ttcaggccat cacgccagtg gtacgggaaa aatcgctctc
     3781 tgccagccca aaattatggc tggagttacc tggtaaccgc ccacaacatg ctgccggaac
     3841 gccggtgctg acactggaaa atctgaccgg cgaaggtttc aggaatgtca gcatgacgct
     3901 caatgccgga gaaattctgg gcctggctgg gctggtgggg gccggacgca cagaactggc
     3961 cgagacgttc tatggtctgc gtactttgcg tagcggacgc attatgctga atggtaaaga
     4021 gatcaataaa ttatccactg gagaacgttt actgcgcggt ctggtttatc tgccggaaga
     4081 tcgccagtca tccggactga atctcgatgc ttcgctggcc tggaacgtct gcgcccttac
     4141 tcataacctt cgtggattct gggcgaaaac cgcgaaagat aatgccaccc tggaacgtta
     4201 tcgtcgggcg ctgaatatta aatttaacca accggaacaa gctgcacgga cattatccgg
     4261 tggcaaccag caaaaaatcc tcattgccaa atgcctggaa gcttcgccgc aagcattgat
     4321 tgtcgatgag ccgacgcgcg gcgtggatgt ctcggcccgt aatgatatct accagctgtt
     4381 gcgcagcatc tccgcacaga atgtggctgt gctgcttatc tcctctgatc tggaagagat
     4441 cgaactgatg acagatcgtg tgtatgtgat gcatcagggc gaaattaccc actctgcact
     4501 gaccgggcgc gatattaatg tcgagactat tatgcgcgtt gccttcggcg atagtcagcg
     4561 tcaggaggca tcatgctgaa gtttattcag aacaaccgtg aaatcacggc attgctggcg
     4621 gtggtgctgc tgtttgtatt acccggtttt ctcgaccgtc agtatttaag tgtgcaaacg
     4681 ctgaccatgg tttatagcag cgcgcaaatc ctgatcctgc tggcaatggg cgcgacactg
     4741 gtaatgctta cgcgcaatat tgatgtttca gtgggttcga ttaccggaat gtgcgcggtg
     4801 ctgttgggga tgttactgaa cgcaggatat tcactacctg ttgcctgtgt cgcgacttta
     4861 ctgcttggtt tgctcgcggg atttttcaac ggtgtcctgg tcgcgtggct aaagatccct
     4921 gccattgttg ccacccttgg cacgttaggg ttgtacagag gcatcatgtt gctgtggact
     4981 ggcggtaaat ggattgaagg gttacccgcc gaactgaaac agctctccgc accgctgctg
     5041 tttggcgttt ccgcgattgg ttggttgacg ataattctgg tggcatttat ggcctggctg
     5101 ctggcaaaga cggcgtttgg acgcagtttt tatgccacgg gcgataattt acagggcgct
     5161 cgtcaactgg gtgttcgtac ggaagccatt cgcattgtgg cattttcgtt gaacggctgc
     5221 atggcggcac tggcgggaat tgtgtttgct tcgcagattg gttttatccc caaccagacc
     5281 ggtaccgggc tggagatgaa agcaattgca gcctgcgtgc tgggcggcat tagtttgctc
     5341 ggcggttccg gtgcgatcat cggtgcggta ctcggcgcat ggttcctgac gcagatcgat
     5401 agcgtactgg tgctgttgcg cattccggca tggtggaacg attttatcgc gggcctcgtg
     5461 ctgctggcgg tgctggtgtt tgatggacgc ctgcgttgtg cgctggaacg taatctacgg
     5521 cggcaaaaat atgcccgctt tatgacgcca ccgccatccg ttaaacccgc ttcgtcaggt
     5581 aaaaaacggg aggccgcata atgcgtattc gctacggttg ggaactggct cttgccgcac
     5641 tgctcgttat tgagattgtc gcatttggtg caattaaccc gcgaatgtta gatctcaata
     5701 tgttgctgtt cagcaccagt gactttatct gcattggcat tgtcgcccta ccgctgacga
     5761 tggtgattgt cagtggcggg atcgatattt cgtttggttc gaccatcggc ctctgcgcca
     5821 ttgcattggg cgtactgttt caaagtggtg tgccgatgcc gctggcgata ctcctgacct
     5881 tactgctcgg cgcattgtgc gggctgatca acgccggatt aattatctat accaaagtta
     5941 acccgctggt gattacgctt ggcacgctgt atctgtttgc cggaagcgct ctgctgcttt
     6001 ccggtatggc cggagcgacg gggtacgaag gtattggtgg attcccgatg gcgtttacag
     6061 atttcgctaa cctggatgtg ctgggactcc ccgttccgct gattatcttc ctgatatgtc
     6121 tcctcgtttt ctggctctgg ctgcataaaa cccatgccgg acgtaatgtg tttttgattg
     6181 ggcaaagccc gcgcgtggcg ctttatagcg cgattccagt taaccgcacc ttatgtgcgc
     6241 tctatgccat gacggggctg gcgtctgcgg tcgccgctgt gctgctggta tcgtattttg
     6301 gttcagcacg ttccgatctc ggtgcgtcgt ttctgatgcc cgccatcacc gccgtggtgc
     6361 ttggcggggc caatatttat ggtggttccg gttccattat cggcaccgcc attgcggttt
     6421 tattagtggg atatttgcag caaggtttgc aaatggcagg agtgccaaat caggtgtcca
     6481 gcgccctttc cggtgcgcta cttatcgtcg ttgtcgtagg tcgttccgtt agcctgcatc
     6541 gccagcaaat taaagagtgg ctggcgcgtc gggccaataa cccattgcca taaaggatat
     6601 cttcatgaca cttcatcgct ttaagaaaat cgccttactt agcgctcttg gcattgccgc
     6661 aatctctatg aatgtgcagg ccgcagagcg tattgcattt attcccaaac tggttggcgt
     6721 gggatttttt accagcggtg gcaacggcgc acaacaagcg ggtaaagagc tgggcgttga
     6781 tgtgacctac gacgggccga cagaacccag tgtttctggt caggtacagt tgattaataa
     6841 cttcgtcaat caaggttata acgccattat cgtttctgcg gtttcgcctg atggcttgtg
     6901 tccggcactg aaacgcgcca tgcaacgtgg tgtgagagtg ctgacctggg actctgatac
     6961 taaaccggag tgccgctctt actacattaa tcagggaacg cccgcccagt tgggaggtat
     7021 gttggtggat atggcggcgc gtcaggtgaa taaagacaaa gccaaagtcg cgtttttcta
     7081 ctcaagcccc accgttacgg accaaaacca gtgggtgaaa gaagcgaaag cgaaaatcgc
     7141 caaagagcat cctggctggg aaattgtcac tacgcagttt ggctataacg atgccactaa
     7201 atcattacaa accgcagaag gaatattaaa agcgtatagc gatctcgacg ccattatcgc
     7261 ccccgacgcc aacgccctgc ccgctgccgc acaagccgca gaaaacctga aaaatgacaa
     7321 agttgcgatt gtcggattca gtacgccaaa cgtgatgcgt ccatatgtgg aacgcggcac
     7381 ggtgaaagaa tttggcctgt gggatgtggt tcagcaaggc aaaatttcag tgtatgtcgc
     7441 ggatgcatta ttgaaaaaag gatcaatgaa aacgggcgac aagctggata tccagggcgt
     7501 aggtcaggtg gaagtctcgc caaacagcgt tcagggctat gactacgaag cggatggtaa
     7561 tggcatcgta ctgttaccgg agcgcgtgat attcaacaaa gagaatatcg gcaaatacga
     7621 tttctgatgt gcattactta accggagtaa gttatggcag atttagacga tattaaagat
     7681 ggtaaagatt ttcgtaccga tcaatcgcaa caaaatatcc cttttatcct gaaaggttgc
     7741 ggtgcgctgg attggggaat gcagtcacgc ttatcgcgga tatttaatcc gaaaacgggt
     7801 aaaaccgtga tgctggcctt tgaccatggt tattttcagg ggccgactac cggacttgaa
     7861 cgcattgata taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc
     7921 ggcattttgc gcagcgtagt tcccccagcg accaataagc cggtggtact gcgggcgtca
     7981 ggagcgaact ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat
     8041 gccgtgcgcc tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa
     8101 catcagtcga tcaaaaatat tattcagctg gttgatgccg gaatgaaagt gggaatgccg
     8161 accatggccg tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc
     8221 gcgactcgaa tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa
     8281 ggttttgaac ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa
     8341 ttaccggagc gcgaggcgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt
     8401 gtggatatgg ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta
     8461 caggcggtgg ttcaccataa cgaaacggca accggaacag gcactcgatc tgatttttgc
     8521 taatgcctca ctgcaatggc tgcccgacca ctacgaattg tttcctcatc tggtttcttt
     8581 acttaatccg cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca
     8641 tgtgctcatg cgcgaagttg cctgggaaca aaactacccg gatcgcgggc gtgagccgtt
     8701 ggctggcgtt catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg
     8761 gcgaaccact tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc
     8821 cactggatta cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac
     8881 gcgctaccat cagatgctgg aagagcagta tccactgcaa gagaacggac agatactgct
     8941 ggcatttccg cgtctgttta ttgttgcccg ccgtacggag taattatcac gtcagctggt
     9001 aatgacgatc gggaagaatt tttgctggaa tttctgcttc atcgttcatc tgtaacaggt
     9061 caatttcaat agcgttgcag atggcatcca gcggtaaatc attgttttca gtaccgaacg
     9121 gatcttccag ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa atcagcacag
     9181 agataaaagg cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa
     9241 acagataaac ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa
     9301 tgcgctcaca gcctgccaga accgctgaaa tatcattaag ccgatcgttg aggctaataa
     9361 actggatatc tgaaagctgc ccattgcggc gctgaaccgc caaccactcc cccattatta
     9421 acaagatacg gttagctgga gagttcgaag ccagtacagg ctgaagatct tcagtcttga
     9481 gataatgagc cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg
     9541 cgaaggcgat ttgcaaccgg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt
     9601 ttacctcgcg cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt tttcgcgctt
     9661 caacgtaacg ggcgtacccg gcattattac gaaaaccaag aaaaatggcg atggcgacac
     9721 cgagaatgct gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca
     9781 tgaaaataac agcgatagaa aagagaaaat tgagtagtaa gcgcgatgat atcttggata
     9841 atactgagcc gtgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca
     9901 tggttatctt caggcgtgaa aaagtcgccc tattaaacgt gattacgatc acattctcaa
     9961 gacgctactt acaaattacc tacaagctta caacattaac aggcgctgca tgtggcatct
    10021 gttaatgata aaggcacata ggatgttgta actaatatag tgaaataaga aacccggtcg
    10081 aaaccgggtt cagaagtaac ggtgttatta gcacaacgga cgtacagctt cgcgcatccc
    10141 tttttcaaga atcgcatcca ggtcattagc aacctgctcg accagaccag gcacttgcgt
    10201 caggtcctgc tcccagtggt ctttttctgc caacacaatc gctaccagtt cctgggtacc
    10261 gataacacgg tcacgatgct ggctccagag ttgctgataa cgttccagcc agtgtgcatc
    10321 gtcctgtacc ggatatgttt ctccgttacg ctcaccacga tagaaggcaa tcaatgccgc
    10381 taatgcgaaa gtaaggcgcg ccggaagtgt gccttttgcc ttctgccgtg ccagcagctg
    10441 cggcaggatg cgagtgcgga acttggtcat accgttgagc gcgatagaca gcagctgatg
    10501 cttaatgtac gggttacgga aacgcccggt gactgcactg gcgaaagatt ccagttcatc
    10561 acgaggcaaa tccagtaccg gaataatttc ttcgtagata gctttttcaa cgaatgcgca
    10621 aatttcagca tcgttcatcg cttcacctac ggtatccagc cctgcctgga acgccaccgg
    10681 caccagcgcg gtgtgcgcac cgttgaggat tgccactttg cgttctttgt acggtttaat
    10741 atcgtcaaca atcagcacgt tgagcggata tttgtccaga cgcagttcag tcgctaagga
    10801 tttcggtccc tgaatcacaa acaggtaaaa gtgttcagcg gtgtcgagaa aaccatcgtg
    10861 ataacccagc tcttcttcca gtttagccac ttcatcgcgc ggataaccgg taacgatacg
    10921 gtccaccagc gtagaacaga agctgttagc ctgatccagc cattgaataa atgcttctgg
    10981 cagtgcccac tcttgcgcat agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc
    11041 gatcaactca cacgggatga tgatccaacc tttatccagc gcaccgttga aatggctaaa
    11101 acgttcgaac agcagacggg tcagttttgc cggataactt accgctggcg cgtcatcgaa
    11161 tttatcgccc gcatggtagc tgatacccgc ttcggtggta ttagagaaaa caaagcgcat
    11221 ttccgggttg tgagccagtt tcaggaattc atcgtattca ctgtagacgc tgatttcacg
    11281 attaaccgag cgaatcagac gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg
    11341 gataatggtg gtatacagac catcctgcgt gctcagtgac ggtgggaatg aagtttcaat
    11401 cggacgaaca acgaccacac cagaattcag atcggtgtgc tcattcagga gatcgatttg
    11461 ccagtcaaca aaggcgcgca ggaagttacc ttcaccaaac tgaatgatac gttctggata
    11521 ctgtgcaccg ggaaaatcgc gacgatttag tgttttcaca atgggttccc ttctgattag
    11581 tcatacaacc tgtttgaatt ggtacgacag gttagcaaac tttaatacgc cgaacccctg
    11641 ttttgatcaa ctcctgatga ttaatgagca gttttatgag aaaagtgtgg cgcggatcat
    11701 ggtttaatcg aggaaatacg ccttttcctg gatcataaag tggtagaaca cattgcattc
    11761 aaatcgcgcg gaatgaataa agatgtcaga caacttcctc accgtaacgc atagtgctgg
    11821 tacggttgcg cccatctttc ttcgagcgat acagacacgt atcggcttcg accatcaatt
    11881 tattaaaatc atcggtcagc gttcggtacg atgcgcgacc actaccgacg ccaatactta
    11941 ccgtgagata aagcgttttt tgttgccagg tgaatggttg cagttcaacg cctttacgga
    12001 ttttttccgc cattagcaga ccatcgacag gattcaccga cggcactgca acagcaaatt
    12061 cttcgccgcc catgcgcgcc accagcccct tatcaccgac aatcttctga atatgccggg
    12121 caaacacgct taacacttta tcgccacatt catgtccata gttatcgtta atgcttttga
    12181 aatagtcgat atcaagcagc atgaccgtca gatgttgtgt ctgtttcagc gatggacttt
    12241 tcaacgcctc ataaagaccg gaccgtgagt aaacctgagt cagaaaatca aagtcggctc
    12301 gcagcgcaac ttgcttcatt agcgaattga tcgctgccac gctaaaagaa accataattg
    12361 ggcatatcgc catcgtggca ataccgagac gtgcggagaa catttccgga atggagaacg
    12421 gcgaaccgac cgagatatca atcaccgaat ttgccaccag tacgatttcc accgcaccgg
    12481 tgacaaaggt caacagacat gttacctgcg gcgtatagcg cactgcacac cagattaatg
    12541 caggcagcgg gaacgccaga ctccccgctc cgccaatgac taccgaggca ataaccgaca
    12601 caataagcgc gatggcgggc atcatctgtt ctgctttaaa gcgcggcagc actccaggaa
    12661 tagccaacgt cagcatacaa ggcacgatca acacgcccgt tgagaattgc tcactgaacc
    12721 agtccgcaag caaaggccag aaatccagac tatcaatact gaccgaacca atcgccccga
    12781 caatagcgca taataatgcg gcaatcagac agtaattaaa tagccgtaag gcacttactg
    12841 gctcatactt atttttcccc agacgcttat cccgcacgac cagtaaggcg acggtaacaa
    12901 taaacatcat attggagaaa ttgataacca gtgaaaccag tccccattcg gtggttatgg
    12961 catcataaac cagcatcgcc acataactga tcgcatagta atgcagtcga ttaagccaga
    13021 catagcgggc aaacaccccc gccattacgc cattcagcgg ccagaaaaga gaaagttcgt
    13081 gaaccagccg taactctgca ccaataaaat agaacagcgt ggttagcgca aaaatagcga
    13141 tgctattacg tagcagatga ccttcctgga acaaacggaa agttgaaatg gggtgtacat
    13201 gcattaatta tctgtccata caattgcggg tggcaattat ctcaacgtaa taagtaaatt
    13261 ctcataaagg atagcatgtt cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct
    13321 attttatcgt tttccgcaat tctcatggct gattcatctg tacaaccagc cagtgataga
    13381 ttaccgccac gatataacgt tgttgttctt cgcttaacga aacaccctgc ggaatgttat
    13441 gccacttagc cagacaaccg cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg
    13501 gatgcccgcg catgggggtt tgcttaccgt cattagccgg taatgccgga gcaagacgtt
    13561 tagcgacaaa atccgccgca tgttgttcga tgactggcgc gcctttctcc aggcaatact
    13621 gacgctcttt catgcccaga cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga
    13681 agagtggatc gagagactgc attaataaac cgaacgccct aactgtttcg tcaattgttc
    13741 aaggacggca atacccgcaa gcgagttacc tgcatcatcc agttccggac tccagacagc
    13801 gatggccatt tcatgcggaa caatcgccac aataccgcca ccaacgccag atttcgccgg
    13861 tagccccacc cgccaggcaa actcccccgc gttctggtac ataccactgg tcgccatcag
    13921 cgcgttaatt tgccgcgcct gcattggcgt caccacaggt tcatcaatat gaatagcttt
    13981 cccctgatta gccagaaaga caaacgtcct ggccagctct acacagctca ttttcagagc
    14041 gcagtaatga aagtagtttt gcagaacggt tgtcacatca tgatggaaat tgccaaacga
    14101 cttcatcagc caggcgatag ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac
    14161 caccgtatcg tatgaaatat cagacacacc gcttaagccg cgcacgactt ccagcatacg
    14221 ttgccgtggt gcgcttaatc gcccttgcaa catatcgcaa accaccagcg caccagcatt
    14281 aataaacgga ttacgcggaa taccctgctc catttccagt tgcactaaag aattgaacgg
    14341 tgatccagac ggatctttgc cgacgcgttg ccagatttcc tcttcggagt aatgacgcat
    14401 ggcgacaacg agactcagca ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc
    14461 tccggcctga aaaagctgtc cgtcaacggt acagatagca atgcccaatc gggaaccgtc
    14521 tactgtagcc agcgccggaa tataatccgc gactttaccc tgaccaatga gcggccgcac
    14581 ttgccgcaag atgttttcta aaattgcatt atccatggcg actgccactt tctactcctg
    14641 gaccgcaggt ctgaaaagac ctgcgagtat atcagagctg aatatgtcgc gtcagatccg
    14701 gtctttccac accgtctgga tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg
    14761 accaaagcca ctctttttca cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc
    14821 attgataaac accccaccgc attccagacg tgccgccatc tgtctggcct gtgtttcgtc
    14881 ggtggtaaaa atggtcgctg aaaggccgaa ctcactatca ttagccagtt ccagcgcatg
    14941 ttctgcatct ttcgcaacgg tgattgccgc aacagggcca aacatttctt cccgaaacgc
    15001 ggtcatttct ggggtaacat tcgccagaac cgttggcgga tagtagttac ctgccccagc
    15061 catcttttcc ccgcccagta acaaacgcgc accctgcgcc agggttttct ccacctgatg
    15121 atgcagctca tcacgtaaat caaaacgagc cattggtccg agagcattct cttcatcacg
    15181 gggatcgccc attttcaagg ctgccgcagc tgccacaaaa cgttcggtaa atgcagaagc
    15241 aattccctct tcgataataa agcgttttgc cgctgcacaa acctgtccgg tattctgata
    15301 acgtccggct accgccgctt taaccgccag ttccagatcg gcatcgttaa gcacaataaa
    15361 tggatccgaa ccgcccagtt ccagtacgca ttttttcagt gccgctccag cctgtgcgcc
    15421 aatagccgct cccgcacgaa cacttccggt caccgtgaca gcagcaatgc gcgaatcttt
    15481 aatcatttga ctgacaccgt cgttgtcggc attcagccag ccatatacgc cttgcgggat
    15541 tcccgcatct ttaaacacct gggcaatgag ctgagcacag cccatcacat tcggcgcatg
    15601 tttaagtaag tagccgttac ctgcaagaat gatgggaacc gcgccacgca tcacctgcca
    15661 taacggaaag ttccacggca taatcgccag aatcgtcccc aacggtcgat actcaataac
    15721 tgcctgctga ttttccacca gcgtaggttc cgccttcagc attgccggac catgttctgc
    15781 ataccagtca cacaaattcg ccgatttcgc cacttcagcg cgcgcctgat tgattggttt
    15841 gcccatttca cgggtgatca tttgcgccat ttcttcgcta cgggcgcgca gagccttacc
    15901 gataccacgc agtttttcag cacgataatc tatatttgtc tcgcgccagt cgcgaaagcc
    15961 tgctgccgcc agctgaagtg cgttttcgat atcgttagcg ccagcccacg gcagcacaga
    16021 aagttgttca cccgtggcag gatttattga aattgcatga gttgccggag taatggtcat
    16081 cggggtatct cctttatgag tcatggtatg aagatacgca gatttactct tgctttaaaa
    16141 tgaataatat taagccactt attcacgaat cgagaatgtt atggatctga cccaactgga
    16201 gatgttcaac gcggttgccg aagctggcag cattacccag gctgcagcaa aagtgcatcg
    16261 cgtaccgtcg aatctcacta cccgtctacg ccagctggaa acagaactgg gggtagatct
    16321 gtttattcgc gagaatcagc gtttacgtct ctctcctgcc gggcataact ttttacgcta
    16381 tagccaacaa attctcgcgt tagtggatga agcgcggagc gttgtcgctg gcgatgagcc
    16441 gcaaggttta ttttctcttg gttcgctgga aagcaccgca gcagtgcgca ttccggccac
    16501 gctggcggaa ttcaaccatc gttatcccaa aattcagttt tcactttcca ccggcccttc
    16561 cggcactatg ctggaaggag tgctggaggg aaaactgaat gcggcattta ttgatgggcc
    16621 cattaaccat actgccattg acgggatacc ggtataccgc gaggagctga tgatcgtcac
    16681 gccacaaggt catgcgccag taatccgcgc cagtcaggtt aacggcagta acatttatgc
    16741 cttccgcgcc aattgttcgt atcgtcgcca cttcgagagc tggtttcatg ctgacggtgc
    16801 cgctccggga actattcacg agatggagtc gtatcacggg atgttggcct gtgtggtcgc
    16861 aggagcaggc attgcgctta ttccacgctc tatgctggaa agtatgccgg ggcatcacca
    16921 ggttgaagcg tggccgttag ctgagcaatg gcgttggtta acaacctggc tggtctggcg
    16981 tcgcggtgcg aaaacgcgtc cgcttgaggc atttattcaa ctactggatg tgcctgactc
    17041 ggcaaaacag ggttaccaat gagctatttt tgatagttct tgcagctcaa atgccctata
    17101 tagtgatgtt ccgatgactt atgactatat ggggcaaata tggttacgcc agtaagcatc
    17161 agtaattaca tatctcttcc tgatgatttt cccgtacgta atattgcacc tcaggtaaaa
    17221 gaagttttaa aggactttat tgatgcgcta agtacaataa tatgtgatga agagtggcgc
    17281 acgtctttaa acatcaactc agccacaaaa aagatattta ataaccttga taacttatca
    17341 ta
//
LOCUS       DAZGOB010000104        17211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGOB010000104 DAZGOB010000000
VERSION     DAZGOB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.104"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..760
                     /gene="ais"
                     /locus_tag="ACHLIF_003634"
     CDS             158..760
                     /gene="ais"
                     /locus_tag="ACHLIF_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO9607577.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(799..1224)
                     /gene="nudI"
                     /locus_tag="ACHLIF_003635"
     CDS             complement(799..1224)
                     /gene="nudI"
                     /locus_tag="ACHLIF_003635"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO9607578.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1503..2045
                     /gene="yfaZ"
                     /locus_tag="ACHLIF_003636"
     CDS             1503..2045
                     /gene="yfaZ"
                     /locus_tag="ACHLIF_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO9607579.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2145..3347
                     /gene="yfaY"
                     /locus_tag="ACHLIF_003637"
     CDS             2145..3347
                     /gene="yfaY"
                     /locus_tag="ACHLIF_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO9607580.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3411..3872
                     /locus_tag="ACHLIF_003638"
                     /pseudo
     CDS             3411..3872
                     /locus_tag="ACHLIF_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3913..4815)
                     /gene="rpnE"
                     /locus_tag="ACHLIF_003639"
     CDS             complement(3913..4815)
                     /gene="rpnE"
                     /locus_tag="ACHLIF_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO9607581.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(5009..6199)
                     /gene="glpC"
                     /locus_tag="ACHLIF_003640"
     CDS             complement(5009..6199)
                     /gene="glpC"
                     /locus_tag="ACHLIF_003640"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO9607582.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6196..7455)
                     /gene="glpB"
                     /locus_tag="ACHLIF_003641"
     CDS             complement(6196..7455)
                     /gene="glpB"
                     /locus_tag="ACHLIF_003641"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO9607583.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7445..9073)
                     /gene="glpA"
                     /locus_tag="ACHLIF_003642"
     CDS             complement(7445..9073)
                     /gene="glpA"
                     /locus_tag="ACHLIF_003642"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO9607584.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9346..10704
                     /gene="glpT"
                     /locus_tag="ACHLIF_003643"
     CDS             9346..10704
                     /gene="glpT"
                     /locus_tag="ACHLIF_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO9607585.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10709..11785
                     /gene="glpQ"
                     /locus_tag="ACHLIF_003644"
     CDS             10709..11785
                     /gene="glpQ"
                     /locus_tag="ACHLIF_003644"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO9607586.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11827..12066)
                     /locus_tag="ACHLIF_003645"
                     /pseudo
     CDS             complement(11827..12066)
                     /locus_tag="ACHLIF_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12064..12168
                     /locus_tag="ACHLIF_003646"
                     /pseudo
     CDS             12064..12168
                     /locus_tag="ACHLIF_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12248..12898
                     /gene="inaA"
                     /locus_tag="ACHLIF_003647"
     CDS             12248..12898
                     /gene="inaA"
                     /locus_tag="ACHLIF_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO9607587.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12952..13206)
                     /gene="yfaE"
                     /locus_tag="ACHLIF_003648"
     CDS             complement(12952..13206)
                     /gene="yfaE"
                     /locus_tag="ACHLIF_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO9607588.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13206..14336)
                     /gene="nrdB"
                     /locus_tag="ACHLIF_003649"
     CDS             complement(13206..14336)
                     /gene="nrdB"
                     /locus_tag="ACHLIF_003649"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO9607589.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14425..16710)
                     /gene="nrdA"
                     /locus_tag="ACHLIF_003650"
     CDS             complement(14425..16710)
                     /gene="nrdA"
                     /locus_tag="ACHLIF_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO9607590.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            16838..16981
                     /gene="ypaB"
                     /locus_tag="ACHLIF_003651"
     CDS             16838..16981
                     /gene="ypaB"
                     /locus_tag="ACHLIF_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO9607591.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4066 a         4194 c         4591 g         4360 t
ORIGIN      
        1 attttttctt atttaggctt tgcatttggc aaaattttga ggcattttgc cgacatcgta
       61 ggatttttaa tattacacca actgctaatt atcgccagaa atgtcgctca attttacggt
      121 aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg ttagcttttt gccgctcatc
      181 attgaagtca aaaaaatatt tcatcatttt actggcgctc gctgcaattg ccggactggg
      241 tactcatgcc gcctggagta gcaatggttt gccacgtatc gacaataaaa cattggccag
      301 actggcacag cagcacccgg ttgtcgtttt gtttcgccat gctgaacgtt gcgatcgttc
      361 aaccaatcaa tgcttgtcag ataaaacagg tattacggtt aaaggtaccc aggatgcccg
      421 tgaactgggc aacgctttta gcgctgacat ccctgatttc gatctttatt ccagtaatac
      481 cgtccggacc attcagtcgg cgacctggtt ttccgcgggt aaaaagctga cggtagataa
      541 acgacttctt cagtgcggta atgagattta tagtgcaatt aaggacttac aaagcaaagc
      601 gcctgataaa aatatcgtta ttttcaccca taatcattgc ctgacatata ttgctaaaaa
      661 taagcgtgat gcgacattta aacctgatta tctggatggt ttagtcatgc atgtggaaaa
      721 aggcaaagtt tatctggatg gggaattcgt taaccactaa attttcaatc tgacagccag
      781 tgatggctgt catcattgtt aaagaagacc tttcaaacgt aacgtttttc gggtggcaac
      841 atttaaatca taatgcacca gatcttcagg ttttacccac gcgtagtcct gaaactcttc
      901 gtttattttc acttctcggt tggcagaaac gcagtcaaaa atcaggtaaa tcatataaat
      961 ctcttccttg cgaccatctg catacgtctt ggtgcgaata tcatcgctga aggtccacgg
     1021 cgtgatttct gtcaaaagca gctgttctcc cagttcttcg cgaatttcgc ggcgtagtgc
     1081 ctcttcaatt cgttcgccag actccacgcc gccacccgaa agcgcccatt gaccggggaa
     1141 aacgccgcga tcgtcggcca ttttacacag caaataagca ccatcatttt gaatcaaagg
     1201 gcatacaata gtccgttgtc gcataggggt attccttata aataaaaaga aaccatgaga
     1261 atacggtaac tcagaaatat gtgaaagtga cagtaatctc ttttgcaatg ataactgcac
     1321 ttattattat ttttgtgtgt atcggtaaag tgtgtttatt ttttcggcgt ttaattattt
     1381 aagaagtatt tagttacaat aagtgactag atcccttttt tcgctcattg aatcctctgt
     1441 gccaggcagt acaatcccgc agctttggga attttgatta attgcttgtg ggaaagggga
     1501 taatgaaaaa aattgcgcta gcaggtctgg ccggaatgct gttggtttct gcatcggtca
     1561 atgcaatgag catcagcggc caggcgggta aagaatacac caatattggt gtcggttttg
     1621 ggactgaatc gacgggcctg gctttaagcg gtaactggac acataacgac gacgacggtg
     1681 acgtcgcggg cgtggggctg gggttgaatc tgcctctcgg gccgttaatg gcgaccgttg
     1741 gcggaaaagg cgtttacacc aacccgaatt acggcgatga aggttatgcc gcagcggtag
     1801 gaggtggttt gcagtggaaa attggcaaca gcttccgttt gtttggcgag tattactact
     1861 ctccggattc gctctccagc ggtattcaaa gttatgagga agcgaatgct ggcgcgcgtt
     1921 acaccattat gcgtccagtc agtattgagg cgggttatcg ctacctgaat ctgtcgggta
     1981 aagacggtaa ccgcgacaac gccgtggctg acggcccgta tgttggggtt aacgccagtt
     2041 tctgatccct tccggcgcgg cattctgtcg cgccattcag tttcttacta cgccttatct
     2101 ccctttgcgt tatagtgatt cgataacaaa agcaggagat aaccatgtta aaagtggaaa
     2161 tgctatccac cggggatgaa gtgttacacg ggcaaatcgt tgacactaac gctgcctggc
     2221 tggccgattt tttctttcat caggggctgc cattatctcg ccgcaatacg gtgggggata
     2281 accttgatga cttagtcacc attcttcgcg aacgtagtca gcacgccgat gtgctgatcg
     2341 ttaacggcgg gctgggaccg accagcgatg atttaagcgc actcgccgct gcgacagcaa
     2401 aaggtgaagg catggtgctg catgaagcct ggctcaaaga gatggaacgc tatttccacg
     2461 aacgtggacg agtaatggca ccgagcaacc gtaaacaagc ggagctgcct gccagtgctg
     2521 aatttatcaa taacccggta ggcaccgcct gtggttttgc cgtgcagctt aatcgttgcc
     2581 tgatgttctt tactcccggc gtaccgtcag aatttaaggt gatggtcgag cacgaaatcc
     2641 tgccgcgtct gcgcgagcgt ttttctttac cacagccgcc ggtttgtctg cgtttaacta
     2701 cctttggtcg ttcggaaagc gatctggcac aaagcctgga cactctacaa ctgccgccgg
     2761 gcgtaacaat gggctatcgc tcttcaatgc cgattatcga actgaaactc accggaccgg
     2821 caagcgagca acaggcgatg gaaaaactgt ggctggacgt taaacgtgtt gccggacaga
     2881 gcgtgatttt cgaaggcacc gaaggactgc ccgcgcagat cagtcgcgaa ttgcaaaacc
     2941 gccagttcag cctgacgttg agcgagcaat tcaccggtgg tttattggct ttgcaacttt
     3001 ctcgcgcagg tgctccattg ctggcgtgtg aagtggttcc ttcacaggaa gaaaccctgg
     3061 cgcaaactgc gcactggatt acagaacggc gggccaacca ttttgccggg ctggcactgg
     3121 ctgtttcggg tttcgagaac gaacatctca actttgcgct agccacacca gacggcactt
     3181 tcgctctgcg tgtgcgtttc agcactacgc gctacagcct ggctatccgt caggaagtgt
     3241 gcgcaatgat ggcactgaat atgctgcgcc gttggttaaa cggccaggat atcgccagtg
     3301 agcatggctg gattgaggtt attgagtcca tgaccttatc tgtctgattc cgccactctg
     3361 tgccggttta ctgcaccggc acattccccc tccggcaaaa cttgtccaac cctccctacg
     3421 gtgagcgtgg tgttggggcc agtgtggcac gggctgcgcg ctggggacgc attgagaatt
     3481 acatggcgca agtcaacgat tcgctttgtc tgttagtgca ggtggaaagt aaaacggcac
     3541 tggataacct ggacgagatc ctcgacgtcg aagggattga tggcgtgttt attggaccag
     3601 cggatctttc tgcgtcgttg ggctacccgg ataacgccgg gcacccggaa gtgcagcgaa
     3661 ttattgaaac cagcattcgg cggatccgtg ctgcgggtaa agcggctggt tttctggctg
     3721 tggctcctga tatggcgcag caatgcctgg cgtggggagc gaactttgtc gctgttggcg
     3781 ttgacacgat gctctacagc gatgccctgg atcaacgact ggcgatgttt aaatcaggca
     3841 aaaatgggcc acgcataaaa ggtagttatt gatatcaaag gcccatgggg atcggctgtg
     3901 ggcctgtgtt aattagtggt tattcgctgc cagatcggct tcgcttagct gggtggccgc
     3961 gagcacctgg tcacgatcaa tgccctgttc cagcatgcgc aaagcaattt taatggcttg
     4021 ttcatgcata ccttcctgcc agccaatttg atgcccttcc tgccgtagcc gttcagcaat
     4081 agtcattaat ctctcctttt gtagtggtga acgttcggca atctcctcaa taaaacgggt
     4141 gaaacgggag gtatcgccgc attgcagcag ataattaaac agtgtttgta gctggctgtc
     4201 attagtgaag cctctaacca aaagcgtggt gatcctgtcg accatgccga ttaaatcgcg
     4261 gtcgcgaata tgcttttgaa tcagttccag cagagctata cgccgatgtt gcatgatctc
     4321 atcgtcaggt acgatggtga tatccaccaa cggaaaagct tcggtgtaca actgccgggc
     4381 aagttgcgga tcgtcaaact catccagcca gttaagtgag tacgggtagg gcgatgtttc
     4441 gccatgataa aacagcaatg gcaccaccag cggaactctg tcatagcctt tatccaggtg
     4501 acgctgcatg gcggcagtgg catagcgcat tagccgaaaa gccatattct tttctgcaga
     4561 gctttgatgt tcaatcacgc agtagatata gccgtcaccg tcgctggttt ccacggacca
     4621 caaaacatcc gagtagtaag cgcgtaaact tttttcaata aaactagtgg gttccaggcg
     4681 taaggtttgc aggttgcaaa gcttgcgcag tggttctggt aaatgtattt cgagaaaatc
     4741 ccgtgcggtt tcgggtgtga acataaaggt tttaaacacc gcatcatgcg gggaggatgt
     4801 tgttgattcg gtcatggttt tccgtcacct gatatttatg atgacgcgac attacgtcta
     4861 tcccgggcct ttgacatctg tcagtttcag cctttgcgag acattttcag aggaattttc
     4921 aaagcgttgc aacgcataca tggtgcgtgg aatcgcgctc gcaaaaatgc ccgataagca
     4981 atgtgcttac cgggcatcaa aaggtcggtt aagccagcgc ctgggccagt agcgtaatcg
     5041 gatgttcgca gcgaaggctc gtggacatct caatctgcca tttgcaggtt tcgcagtcgg
     5101 tgatgaccag atccgcgccg ctctcttcta tctggcggaa cagtggcgca ccgatggctt
     5161 gtgaggtggg gtagttctct tttttgaaac cgtaagtacc cgcaataccg cagcactggg
     5221 aatccagcac cgttaactca agccccggga ttttacgcaa cagctccagg gtgtagagcg
     5281 tccagcccat tttttccata tggcacggag tgtgataaac cactttcagc ggcagcggtt
     5341 tcagcggtaa cgttttgcct tcgtccagct tgcgccacag ccagcgggtt gccagttcga
     5401 tatgatcgcg caagcctttg ttgtcgacat tcagcacttc cgggtattcg tcgcgcaggg
     5461 caaatgtaca ggttgaggag gtggcaatca ccggaatgcc ttttactccc acagcttcgc
     5521 ggatcgactc tacattcgta attgcctgtt tgcgtgcttt atcggtaaag ccgttggcga
     5581 ttagcggtac gccgcagcat ttttctttgc tgagcagttg tacaccggta cccattgcgt
     5641 tgagcacttt aattaaatct ttacctaact gcggatggtt gtagttaacg aagcagccgt
     5701 gaaagaaagc gacctggtct ttatattgtg cttgctgagc cgctatgctg cgataccagc
     5761 gacgaaacgt accgaaggag tatttcggta gcgtgcggcg atgatcgatt tttaacgccg
     5821 catcaagcag ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt ggtgcgaacg
     5881 gcgtggaaac gctacccatc aggtcggtat gactcaacac aaaattacgc agcgacgggc
     5941 gcgtggtgtc atattttgcc cgcgcgcgct ggataatatc gccaatcttc acatcggacg
     6001 ggcaggcgac ttcacaacgt ttgcagttga tgcaatattt cagcgcctcg tcatacagtg
     6061 cgccatcttt caaacgcaga cgctcgccat ccggcccggc ttgttttggc cctggataac
     6121 cgggattcac ccggctcacc gggcaggcgg tggtgcagac ggtgcactta atgcagtttt
     6181 cgaagctggt gtcgttcatt gttggcctcc tgcgcgttgg gcaatctgtt gagcggcatg
     6241 taaagcactg acggcacaaa caccgccgcc gcatccctgg gcgatgggat caaatccgcc
     6301 cagcaccgaa ccgatggcaa acaggttctc aatggtttgc cctgcctgcg acgggcgtag
     6361 cgtctgatca gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa aatcgccctt
     6421 ataccattca ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg gctcgcgaat
     6481 gccgttacgt tccgctacca gtccgccact aaagaaactg ccgctggcga gaaccgcgaa
     6541 acgtggacgt agcggaatat cggcgtgatt gcgggtccag atttcattca ctacgccatt
     6601 tttacaggtc acttttttca cttcatcgcc cggcatccac acgccaccct ggcgcacaaa
     6661 ctggcgctgt aactggtttt gcagacgaat gcccagcacg gaaggcggca gcgttggcaa
     6721 aagcatcagt gaacaaggta gtttttcatt caaccaacgc cacagtttgt catcggccag
     6781 gccgaagcag gcgggcatca ggatcatttc gcaggtattg gcgacaggaa taagcgcatc
     6841 aagtaacagc ggccagtttt cttcattatc aaggaaacgg gcgatattca ccgcgcgaaa
     6901 ttcggtggcg ttattgcgca ggacatccag ttccggcagc tctatttctg cggtttcaac
     6961 cgaaagatcc agttcgcgca acgaagccgc cgcaagatgc gcctggaaat ccatcagccc
     7021 gcttatcccc actacacata tcttcttcgc gggtagcggc cagacgggaa cttctggcga
     7081 acttaaccag gttgcacgca aagtgccgag cggggtaacg cgctgatggg ccagttccac
     7141 gctgccctgc aattgcgcac cgctttcagc gatcaatgcc tgcgcctggc aagcgagatc
     7201 gagcacgcgt tgtggttcga gaagactgta aggatgggct ggtgcctgct gacgcaaaga
     7261 ttccagtcca ctgtgaatgt ctgtcaccgg ttgaccatca ggcagatggc tcagcaaatc
     7321 cagcgaaccg gatgagaaat gcagcgcgct ttgaccacgc gtgacaatgg cacagcgcag
     7381 gccgtgtttt tgcagttgca ggccacagag taatccggcg aggccgccgc ccataatgac
     7441 agtatcaaag cgcatctttc tgctccttct ccagaccaca caatccctga taaacccagc
     7501 gggtaaattc gctttcgcgc agtgcatctc cccaggcgat gggttgcacg cctttccagc
     7561 gttcgttaag gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg acgttaaaac
     7621 gttgcagcag tccggcagcg cggcaggcgc agagttcgcc ctggcaggta cccatcccca
     7681 cacgggtacg acggcgtaaa tccagcaggc tattaacgtt taaattttct accgcgtact
     7741 gaacttcacc cgcagttacc gcttcgcact cacataccag gctacgatgc agacggcctt
     7801 cgctcagcca ggcaggcgtg cgatcgccat gacgataaac cgcagaaccg cgcagcgggg
     7861 caggcaggga gatgacttta cgcaaggtaa cttcagcggg ttcttgtgaa ccaggcagtg
     7921 ccagatcggc agtcgtacag gggcgcgtgt tgcccagttt gcggcatacc gcgtcggtag
     7981 cccattcagc catcagccga taggtcatca gtttgccacc ggtgatggtg ataaatccgt
     8041 ccagaccatc gcgttcagca tggtcgagca gcacgatgcc acggctgacg ttacgtccgc
     8101 tcgggtcgtc atcgctggca accagcgggc gcacgccaga ataggcccgc aaaatgcgcg
     8161 ttttcgccat cacgggggcc agtttttccc cttcacgcag cagaatatca acctcttctg
     8221 ccgtcactcg attatcgtca atctcgttgt aatcaatacg taaagaggtg gtgccaatca
     8281 gcgaaatggt atcgccaggc accagaatat cggcgtcgga aggtttacgg cagcggttga
     8341 tcacatgctg gttaatgcgg tgatccatga tcagcagcga tcctttcgcc gggaacatgc
     8401 gaatgcgcag atcggcatat tcggcaatgt gttgccccca gatcccagcg gcattaacca
     8461 cgacaggtgc atgaagggcc tgagtttcgc cggtgagatg gttacgtaca cgaacaccgc
     8521 acaccgtcgc gccttcacga atcagccccg tgacttcatg agcggtaagg ataacggcac
     8581 cgtgttcttt ggcatccagc atgtttgctg cggtcagacg aaatggatca acggtgccat
     8641 ccggaacttt caccgcgcca atcagtgccg ggttaacggc aggttcgata atgcgcgctt
     8701 gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga ataaaagtgg
     8761 cctggaatga gaggtcatct tccggcaggg tgataaacag gccgttggtt ggttcaacgc
     8821 agtgacgtgc aatgcgtttc aggatctggt tttcactaat gcattcgcgg gccgattccg
     8881 catcggttac cgcatagcgc gcaccgctgt gcagcaggcc gtggttacgc ccggtggcac
     8941 cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg gcacagtcgc
     9001 gggcaatccc ggctcccgtt gcgccgccac caatgataat cacgtcactt gattgcgagt
     9061 cgcgagtttt cattgttatc cctctgaagt tcgtttttta ccatttagcc atagtaaaaa
     9121 catgaattgt ttgatttcgc gcatattcgc tcataattcg aaagtgaaac gtgatttcat
     9181 gcgtcattct gaacattttg taaatcttat ttaataatgt gtgcggcaat tcacatttaa
     9241 tttctgaatg ttttcttaac atcgcggcaa ctaaagaaac ggcaggttct cccactgaat
     9301 cagtctgtta atcataaata agaccacggg ccacggaggc tttcaatgtt gagtattttt
     9361 aaaccagcgc cacacaaagc gcgcttacct gctgcggaga tcgatccgac ttatcgtcga
     9421 ttgcgctggc aaattttcct ggggatattc tttggctatg cggcttacta tttggttcgt
     9481 aagaactttg cgcttgctat gccttatctg gtcgagcagg gattctctcg cggtgattta
     9541 ggtttcgcgc tttcggggat ctcgatcgct tatggatttt cgaaattcat aatgggttcg
     9601 gtatcggatc gctcgaatcc gcgcgttttc ctgcccgcag gtttgattct ggcggcggca
     9661 gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga gcattgcggt gatgtttgta
     9721 ctgttgttcc tctgcggttg gttccagggg atggggtggc cgccgtgtgg tcgtactatg
     9781 gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa ctgtgcgcac
     9841 aacgtcggtg gtggtattcc gccgctgctg ttcctgctgg ggatggcctg gttcaatgac
     9901 tggcatgcgg cgctctatat gcctgctttc tgcgccattc tggtggcatt attcgccttt
     9961 gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc cgatcgaaga gtacaaaaat
    10021 gattatccgg acgactataa cgaaaaagcg gaacaggagc tgaccgcgaa gcaaatcttc
    10081 atgcagtacg tgctgccgaa caaactgctg tggtatatcg ccatcgccaa cgtgttcgtt
    10141 tatctgctgc gttacggcat cctcgactgg tcaccgactt atctgaaaga ggttaagcat
    10201 ttcgcgctgg ataaatcctc ctgggcctac ttcctttatg aatatgcagg tattccgggc
    10261 actctgctgt gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg ggcaaccggc
    10321 gttttcttta tgacactggt gaccatcgcg actatcgttt actggatgaa cccggcaggt
    10381 aacccaaccg tcgatatgat ttgtatgatt gttatcggct tcctgatcta cggtcctgtg
    10441 atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg tacggcagcg
    10501 ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg cggcgagcgc gattgttggc
    10561 tacaccgtgg acttcttcgg ctgggatggc ggctttatgg taatgattgg cggcagcatt
    10621 ctggcggtta tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca tgaacaatta
    10681 ctgcaaaaac gcaacggagg ctaatggcat gaaattgacg ctgaaaaacc ttagcatggc
    10741 gatcatgatg agcactatag tcatgggaag cagtgcaatg gcggcggaca gcaacgaaaa
    10801 aatagtcatc gcccatcgcg gtgccagtgg atatttgccg gagcatacgc tgccagcaaa
    10861 agcgatggcg tatgcgcagg gagcggatta tctggaacag gatttggtga tgaccaaaga
    10921 cgaccatctg gttgttctgc atgaccatta tctcgatcgt gttactgatg ttgccgatcg
    10981 tttcccggac cgggcgcgca aagacggtcg ttactacgcg atagatttca cgctggatga
    11041 aattaagtcg ctgaaattta ccgaaggttt cgatattgaa aacggtaaaa aagtgcagac
    11101 ttatccgggg cgtttcccaa tgggtaagtc cgacttccgg gtgcacacct ttgaagaaga
    11161 gattgaattt gttcaggggt taaatcactc taccgggaaa aatatcggta tttatcctga
    11221 aatcaaagcg ccgtggttcc atcatcagga agggaaggat attgcggcaa aaacgctgga
    11281 agtgctgaag aaatatggtt acaccggtaa ggacgacaaa gtttatttgc aatgttttga
    11341 tgctgatgag ctgaagcgta ttaagaatga gctggaacct aaaatgggca tggatctcaa
    11401 tctggtacag ttgattgcct ataccgactg gaatgaaacg cagcagaaac agccggatgg
    11461 aagctgggtt aattacaact acgactggat gtttaagccg ggtgctatga aacaggtggc
    11521 ggaatatgcc gacggaatcg ggccggatta ccatatgttg attgaggaaa catcgcagcc
    11581 gggtaatatc aaactcactg gcatggtgca agatgctcag cagaataaac tggtagtgca
    11641 tccttatacc gtgcggtcag ataaactgcc tgaatacact actgatgtga atcagttata
    11701 tgatgctctg tataacaaag cgggtgtaaa tgggctgttt actgatttcc ctgataaggc
    11761 agtaaaattc cttaataaag agtaatgcta ctgcaggatg gcgttcatgc gccatcctgt
    11821 ctcacatcaa atttttccat tcaccacgag ttgttccgta cacgtttgta gtaaaaacag
    11881 tggactgcct tctaccggct tttctttagc caacaacgaa atctgtcgat agaaggttgg
    11941 ttcgtgagga acggaacaca attcacctgc aatgcttttc agcgttaact ctggttgtaa
    12001 ggcgatgcct aatccctgtc gaataaactt catcgctgta tccgactgat taaattcaca
    12061 ttttatctac tgggcgcact attagtggta gtggctggtg gaatctatgc gctgcaaatg
    12121 aagggtaaaa gcggtgtcac gctagtagtg gcaaaagaaa ttcattaata cgacacgttt
    12181 cattaagatt ttcctcaggt aaccacctat agtcattgtc gttaataaaa atgacaggcg
    12241 atagggtatg gcagtttcag caaagtacga cgaatttaat cactggtggg caacagaggg
    12301 cgactgggtt gaagagccta actatcgccg taatggtatg agcggggtgc aatgcgtcga
    12361 gcgcaacggc aaaaagctgt atgtaaagcg catgacgcat catctgtttc attccgtacg
    12421 ttatccgttc ggccgaccaa cgattgtccg tgaggttgcg gtaattaaag aactggaacg
    12481 ggccggtgtc atcgtgccga agatcgtttt tggtgaagcg gtgaaaattg agggtgaatg
    12541 gcgagcgttg ctggtgactg aagatatggc ggggttcatc agcattgctg actggtatgc
    12601 ccagcatgca gtatcgcctt attctgacga agtacggcaa gccatgttga aagcagtggc
    12661 gctggcgttt aagaaaatgc atagcattaa tcgtcagcat ggctgttgtt atgttcgcca
    12721 tatctatgtg aaaacagaag gtaatgcaga agctggtttt cttgacctgg agaaaagtcg
    12781 tcgccgcttg cgtcgcgata aagcgattaa tcatgacttc cgccagctgg aaaaatatct
    12841 ggagccaatc cccaaagcgg actgggagca ggtgaaagcg tattactacg ccatgtaatc
    12901 gttgtaggcc agataagacg cataagcgtt gtatctggca ctacaaccaa ttcacatctc
    12961 gatttcaata tcgccttttg cccggcaaca acagggcaaa atttcccccg gctgaataaa
    13021 ggctaacggt tcggcaatcc agtcaacctg acctgcaacc agacgggtgc gacaggagcc
    13081 gcagtaacct tcgcgacact gatactcaac cgccacattg tgggattcca gcgccgccag
    13141 aagggaaggg tgttcatcct ggcacagcag ttgtgtgcca gtgatgcgca gggtaacgcg
    13201 ggccatcaga gttggaagtt actcaaatcg tcggtgtcca cttccgcgtc aatctgcccg
    13261 accagataag aactgacttc cacttcctgc ggagcaacct gcacgttatc agacaccagc
    13321 caggtgttga tccatgggat cgggttggag cgcgtctgga acggcagatc caaaccgact
    13381 gcctgcatac ggatattggt gatgtattca acgtactggc agagaatgtc tttattcaga
    13441 ccaatcatcg aaccgtcgcg gaacagataa tccgcccagt ctttctcctg ttgagctgcc
    13501 tgaacaaaca ggtcatagca ctcctgctta cactcttcgg caatttccgc catctcagga
    13561 tcgtccgcgc cgctgcgcag cagattcagc atatgctggg tgccggtcag gtgcagggct
    13621 tcgtcgcggg caatcaggcg aataattttg gcgttgcctt ccatcaattc gcgttctgca
    13681 aatgcgaagg aacaagcaaa gctgacgtag aaacgaatcg cttccagcgc gttaacgctc
    13741 atcaggcaga gatacagttt tttcttcagc tcgcgcaggc taacggtcac agttttaccg
    13801 ttaacggtgt gggtaccttc gcccagcaga tgccagtagc tggtcatttc gatcagctca
    13861 tcgtaatagc tggagatccc ttccgcacgt ttctggatct gctcgttggt gacgatatcg
    13921 tcaaacacaa cagacggatc gttaacgata ttacgaatga tatgagtata ggaacgggaa
    13981 tgaatcgttt ctgagaacgc ccaggtttcg acccaggtct ccagttccgg gatcgagatc
    14041 agcggcagca gtgccacgtt cgggctacgg ccctgaatgg aatccagcag cgtctgatat
    14101 ttcaggttgc tgataaagat gtgtttttcg tgttcaggca gtgcctggta atcgatacgg
    14161 tcgcgagaaa cgtcaacttc ttccggacgc cagaagaaag agagctgctt ttcgatcagc
    14221 ttttcgaaga tgtcatattt ttgctgatcg tagcgagcca cgttgaccgg ctgaccaaag
    14281 aacatcggtt ctttgagctg atcatttttc gtctgtgaaa aggtggtata tgccatgagt
    14341 gtgtcctgtg gttttattac ccctcaccct aaccctctcc cgcaagggga gaggggacgg
    14401 tttggtgcaa aggctggggg attatcagat cttacatgcg ccgctttcgc agccatcgtc
    14461 ctggattgac ggtaccagat cgtcctgcgc gtcttcagcg ccgtcacggg tgttctgata
    14521 atacagcgtt ttgaccccga atttgtaggc ggtgagcagg tctttcagca actgctgcat
    14581 cggcactttt cctgacggga aacgtgacgg atcgtagttg gtgttagcag agatcgactg
    14641 atcgataaat ttctgcatga tacctaccag ttgcaggtag ccatcgttac ccggcatttc
    14701 ccacagcagc tcataggcgt cgtgcaggtg ctcgtagtcc ggcaccacct ggcgcaaaat
    14761 accgtctttc gacgctttga tgctgacgta accgcgcggc ggttcaatac cgttagtggc
    14821 gttagagatc tgtgaagaag tctctgacgg catcagggca gaaagcgtgg agttacgcag
    14881 gccgtgcgtt ttgattgatt cacgcaatgc ttcccagtcg taatgcagcg gctcattagc
    14941 gatggtatcc agatctttct tataggtatc gatcggcagg atccctttcg cgtaagtggt
    15001 ttcgttaaac cacgggcacg cgccttgctc ttttgccagc tcgttagacg ctttcagcag
    15061 gtaatactga atcgcttcga aggttttatg cgtcaggttg ttggcgctgc cgtcggagta
    15121 acgcacaccg tgcttcgcca ggtagtaagc gaagttgatc acaccaatac ccagcgtacg
    15181 acgacccatc gctccacgtt tggcggccgg gatcgggtaa tcctgataat ccagcagcgc
    15241 gtcaagtgca cgaaccgcca gaattgccag ctcttccagt tcatccaggc tattaattgc
    15301 gcccaggttg aaagcagaca gcgtacacag cgcgatttca ccgttctcgt cgttgacgtc
    15361 gttcagcggt ttggtcggca gggctatctc caggcacagg ttagactgac gcactggcgc
    15421 gatggccgga tcaaacgggc tatgggtatt gcagtggtca acgttctgaa tatagatacg
    15481 accggtagac gcacgttcct gcatcatcag cgagaacagc tcaacggctt tcacacgctg
    15541 cttgcggatg ctgtcgtctt tctcatattt ggtatacagg cgttcaaact cttcctgatc
    15601 ggcgaagaat gcgtcgtaca gccccggtac gtcggacggg ctgaacaggg tgatatcttc
    15661 acctttcagc aggcgggtat acatcagttt gttgatctgt accccgtagt ccatatggcg
    15721 cacgcggttg ccttccacac cacggttgtt tttcaacacc agcaggcttt ccacttccag
    15781 atgccacatc gggtagaaca gcgttgccgc accgccgcgc acgccgccct gagagcagga
    15841 tttcaccgct gtctggaaat gtttgtagaa cggaatacag ccggtatgga acgcttcacc
    15901 accgcgaatc gggctaccca gcgcacgaat acgcccggcg ttgatgccga tcccggcacg
    15961 ctgggaaacg tatttcacaa tcgcgctgga ggtagcgttg atggaatcca gactgtcacc
    16021 gcactcgatc agtacgcagg agctgaactg acgagtcggg gtacgcacgc cggacatgat
    16081 tggcgtcggc agcgaaattt taaatgtgga aaccgcgtcg taaaaacgct tcacatattg
    16141 caggcgcgtt tcacgcgggt agttcgagaa caggcacgcg gcaaccagaa tataaaggaa
    16201 ctgggcgctt tcgtagattt cgccggtcac gcggttctgt accagatatt tgccttccag
    16261 ctgcttaacg gcagcataag agaaggtcat atcacggtcg tgatcgataa acgtgtccat
    16321 ctgcttgaac tcttcttccg tgtagtcttc cagcagatga ttatcgtatt tgcccatctc
    16381 gaccattttc accacgtggt cgtacagcgc aggcggctca aactggccgt aggctttttt
    16441 acgcaggtgg aagatcgcca ggcgcgcggc gagatactga taatccggcg catcacggga
    16501 gatcaggtct gcggccgcct tgataatggt ttcgtggatg tcagaggtct tgataccgtc
    16561 ataaaactga atgtgggagc gcagctcgac ctgggaaatc gaaacgttat gcagtccttc
    16621 tgccgcccaa tccagaacgc gatggatttt gtcgagattg atgcgctctg tgctaccgtc
    16681 gcgctttgtc accagcagat tctgattcat gtatgtcgta cctgtttttg gaattctttc
    16741 cgcaatagtt tcatgcagac tacctgtagt acgggaagct gcttagaaca ctatatatag
    16801 ggggtatgtt tgggattcac tgcaagatag tgtgaaaatg gccctcttgc aagtgcataa
    16861 ctttgtggat aactcaggaa ggaaaaagtg gctttcgcgc accttaggtc agacaaggtg
    16921 tccgggaaag tcaatgggaa gagaaaaatt tgttaaaaat aactgttcgg cgatttcttg
    16981 aacggcagaa taggttggat aaattgatat agatggttta ttaatctttt tgctaatgat
    17041 tgcaaaaatt gatctaagca cgagtcatca cataatctga tgtatcagtt gtggtcaata
    17101 tataagcgaa tgtaagcgat tgttgatgtc gaaatggttg ttttctattc ggttattgtt
    17161 aaatgcgaag ccattttgtt aatttacctt atattataag aaaattattc a
//
LOCUS       DAZGOB010000105        17208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGOB010000105 DAZGOB010000000
VERSION     DAZGOB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.105"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..593
                     /locus_tag="ACHLIF_003652"
     CDS             9..593
                     /locus_tag="ACHLIF_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9607592.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            673..1368
                     /locus_tag="ACHLIF_003653"
     CDS             673..1368
                     /locus_tag="ACHLIF_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9607593.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1398..3914
                     /locus_tag="ACHLIF_003654"
     CDS             1398..3914
                     /locus_tag="ACHLIF_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9607594.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQNLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3925..5016
                     /locus_tag="ACHLIF_003655"
     CDS             3925..5016
                     /locus_tag="ACHLIF_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9607595.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5059..5919)
                     /gene="rsmI"
                     /locus_tag="ACHLIF_003656"
     CDS             complement(5059..5919)
                     /gene="rsmI"
                     /locus_tag="ACHLIF_003656"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO9607596.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5983..8019
                     /gene="lpoA"
                     /locus_tag="ACHLIF_003657"
     CDS             5983..8019
                     /gene="lpoA"
                     /locus_tag="ACHLIF_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO9607597.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7977..8372
                     /locus_tag="ACHLIF_003658"
     CDS             7977..8372
                     /locus_tag="ACHLIF_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO9607598.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8392..8982
                     /gene="diaA"
                     /locus_tag="ACHLIF_003659"
     CDS             8392..8982
                     /gene="diaA"
                     /locus_tag="ACHLIF_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO9607599.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            8992..9567
                     /gene="dolP"
                     /locus_tag="ACHLIF_003660"
     CDS             8992..9567
                     /gene="dolP"
                     /locus_tag="ACHLIF_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO9607600.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9681..10721)
                     /locus_tag="ACHLIF_003661"
     CDS             complement(9681..10721)
                     /locus_tag="ACHLIF_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO9607601.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMLYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10794..11429)
                     /locus_tag="ACHLIF_003662"
     CDS             complement(10794..11429)
                     /locus_tag="ACHLIF_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO9607602.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11557..12075
                     /gene="yhbO"
                     /locus_tag="ACHLIF_003663"
     CDS             11557..12075
                     /gene="yhbO"
                     /locus_tag="ACHLIF_003663"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO9607603.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12055..12498)
                     /locus_tag="ACHLIF_003664"
     CDS             complement(12055..12498)
                     /locus_tag="ACHLIF_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO9607604.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12549..12851
                     /gene="yhbQ"
                     /locus_tag="ACHLIF_003665"
     CDS             12549..12851
                     /gene="yhbQ"
                     /locus_tag="ACHLIF_003665"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO9607605.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12838..13341)
                     /locus_tag="ACHLIF_003666"
     CDS             complement(12838..13341)
                     /locus_tag="ACHLIF_003666"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9607606.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13335..13859)
                     /locus_tag="ACHLIF_003667"
     CDS             complement(13335..13859)
                     /locus_tag="ACHLIF_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO9607607.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14068..15063
                     /locus_tag="ACHLIF_003668"
     CDS             14068..15063
                     /locus_tag="ACHLIF_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO9607608.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15072..15950
                     /locus_tag="ACHLIF_003669"
     CDS             15072..15950
                     /locus_tag="ACHLIF_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO9607609.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16156..17163
                     /locus_tag="ACHLIF_003670"
     CDS             16156..17163
                     /locus_tag="ACHLIF_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO9607610.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
BASE COUNT         4336 a         4561 c         4354 g         3957 t
ORIGIN      
        1 ggttataaat gaataaagtt acaaaaacag ctattgtggg cttacttgcg cttttcgcag
       61 gtaatgccgc tgcaaccgat ggtgaaatag tttttgatgg cgagatttta aaatccgctt
      121 gtgaaatcaa tgactctgat aagaaaattg aagttgctct tggtcactat aatgccgaac
      181 agtttcgttc tgttggcgat cgtagcccaa aaattccatt cactattcct ttagttaact
      241 gcccagtgac tgggtgggag cacgataacg gcaacgttga agcgtctttc cgtttatggc
      301 tggaaacgcg ggataatggc accgtcccta acttccctaa tctggcaaaa gtgggctctt
      361 ttgctggcac agcggcaaca ggtgtgggta tccgtattga cgatgcggaa agcggaaacc
      421 ttatgccact gaatgctatg gggaatgata acacggttta tcagatccca gcggactcca
      481 atggtattgt caatgttgac ctcatcgctt actacgtatc aaccgttgaa gcatcagaaa
      541 tcaccccagg ggaagcagac gctgtcgtta acgtaacgct ggattaccgt taatacgtta
      601 cggcgttatc tgacctttca gataacgccc ttttccttcc tctttctcgt tgtatcaggt
      661 tgaaaaataa ctatgtcaaa acgaacattc gcggtgataa taaccttgtt gtgtagcttc
      721 tgtatcggcc aggcgcttgc agggggtatc gttttacagc gaacgcgagt gatctatgat
      781 gccagccgca aagaggctgc gttacctgtc gcaaacaaag gcgcagaaac gccttattta
      841 ctgcaatcat gggtagataa tatagatggt acaagccgtg ccccatttat tataacccca
      901 ccgctatttc gtcttgaagc cggcgatgac tcatcactgc gaattattaa aacagctgat
      961 aacctgcctg aaaataaaga gtcgctgttc tacattaatg ttcgtgccat tccagcaaag
     1021 aaaaaatcag atgatgttaa tgctaacgag ttgacgctgg tatttaaaac acggatcaaa
     1081 atgttttatc gccccgcaca cctgaaggga cgggtaaacg atgcgtggac atcactggaa
     1141 tttaaacgta gtgaccattc actcaatata tataacccaa ctgaatatta cgtcgtattt
     1201 gccggactgg cagtcgataa aaccgatctc acaagcaaaa ttgaatatat cgcgcccgga
     1261 gaacataaac agttaccact tcctgcatct ggcggaaaga acgtgaaatg ggctgcaatc
     1321 aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca aatataaaaa
     1381 acacaggtca tcagggaatg ccacaacgac accaccaggg acataaacgc acaccgaaac
     1441 agttggcgct catcatcaaa cgctgtttgc cgatggtgct cactggcagc ggcatgcttt
     1501 gcactaccgc taacgccgaa gagtattatt tcgaccccat tatgctggaa actacaaaaa
     1561 gtggtatgca aacaactgat ctgtcacgtt tttcaaaaaa atacgcacaa ctaccaggaa
     1621 cttatcaggt tgatatctgg ctgaataaaa agaaggtttc acagaaaaaa attacattta
     1681 ccgccaatgc agagcaactt ctgcagccac agtttacggt agaacaacta cgtgagctgg
     1741 gtattaaggt ggatgaaatc ccggcgctgg ctgaaaaaga tgacgatagc gtgatcaact
     1801 cgcttgaaca aatcattccc ggtacagctg ctgaatttga tttcaatcat cagcgactta
     1861 atttgagcat tccccaaatt gcactgtacc gtgatgcaag aggttacgtc tccccttctc
     1921 gttgggacga tggtatacca acgctgttta ccaactactc gtttacaggt tctgataacc
     1981 gttaccgcca gggcaatcgt agccaacgac agtacctgaa tatgcaaaat ggtgctaatt
     2041 ttggcccctg gcgattacgt aactattcta cgtggacacg caacgatcag gcgtcaagct
     2101 ggaacactat cagtagttat ttacaacgtg atatcaaggc gttgaagtct cagttgcttc
     2161 tgggagaaag cgccaccagc ggcagtattt tttccagcta cacctttact ggcgtgcaac
     2221 tcgcttccga cgataatatg ttgccaaaca gccagcgcgg atttgcccca acggtacgcg
     2281 gtatcgcaaa cagtagtgca atcgtgacta tcaggcaaaa tggttatgtg atctatcaaa
     2341 gcaacgtgcc agcgggtgcc tttgaaatta acgatctcta cccctcttcc aacagcggcg
     2401 atttagaagt cacgattgaa gaaagtgacg gtacgcaacg tcgctttatc cagccttatt
     2461 cttcattacc catgatgcag cgacctgggc atctaaaata tagcgcgacc gctggacgct
     2521 atcgcgctga tgcaaacagt gatagcaagg aacccgaatt tgctgaagcc acggcaatat
     2581 atggtttgaa taatactttt acgctgtatg gcggcctgct cggttctgaa gattattatg
     2641 cgctggggat cggtatcggc ggcacacttg gcgcactggg cgcgttgtcg atggatatca
     2701 acagagctga cacccaattc gataaccggc actcttttca tggctatcaa tggcgtacgc
     2761 agtacatcaa agatatcccg gaaaccaaca ccaatatcgc tgttagctac tatcgctata
     2821 ccaacgatgg ctattttagt tttgatgaag ccaatacccg taattgggac tataacagtc
     2881 gccaaaaaag tgaaattcaa ttcaacatca gccagacaat atttgatggg gtaagtctgt
     2941 atgcctccgg ttcgcagcaa gactattggg gcaataacga taaaaacagg aatatctcag
     3001 ttggggtttc cggtcagcaa tggggaattg gttacagcct gaattatcaa tacagtcgct
     3061 acacggatca aaataatgac cgcgcactct ctttgaatct cagtattccg ttagaacgct
     3121 ggttaccgcg tagccgggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
     3181 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
     3241 aaaatctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
     3301 atggcacctt cagtgccgga tacagctacg gtaatgacag cagccaatac aattacggcg
     3361 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
     3421 cttttgcgct tatcgatgct aatggggctt ctggcgtgag gatacaaaac tatccgggga
     3481 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttac caggaaaacc
     3541 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgagcaa acaacacagt
     3601 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
     3661 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
     3721 gcaacgatga gacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
     3781 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
     3841 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacgg
     3901 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
     3961 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagctgacag cactagcggt
     4021 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
     4081 ggacgggagt ttacctctgc cacgctaagt agcactaact ggcaatacgc ctgtacctgc
     4141 tctgcgggta aggcagttaa acttgtctat atggtcagcc ctgtacttac caccactgga
     4201 catcaagcag gatattacaa actcaatgat agtctggata ttaaaaccac attacaggcg
     4261 aatgacattc caggactcgt gactgaccag accgtttctg ttaacacccg attcacacag
     4321 ataaaaagca acaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     4381 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     4441 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcatgg
     4501 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     4561 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaggtt
     4621 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     4681 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     4741 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccaga
     4801 cgaagaagtt cagacaatgc gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     4861 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     4921 atcaacatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     4981 cagggcaccg ccaccattac cgtcatcgtg cggtaaaaga agacgttgcg ttttgattaa
     5041 cgcaacgctt cggcactctt acccctgctg ctccagcgca tacttataca gcgcattttt
     5101 cttcacgccg tgaatttctg ccgccagcgc cgccgctttt ttcaacggca gttctgcctg
     5161 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     5221 ttcgataatc agcaccattt cgcctttgcg acggttttca tcttctttta cccacgccag
     5281 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     5341 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     5401 gtgggtggat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     5461 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagagggtaa
     5521 acccgcggcg cttaacgcag tgatagcagc acacggcccg ggtagcggca ccacgcaaat
     5581 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     5641 ggcatcggaa accagcgcaa tgttctgccc ttcttgcagc ttcgccagca gcgtttcggc
     5701 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     5761 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     5821 ctctaacgca cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     5881 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     5941 ttaatattga gcattgcgta aaaaaatatc actggataca ttatggtacc ctcaacattt
     6001 tctcgtttga aagccgcgcg ttgtctgcct gttgttctgg cagccctgat tttcgccggt
     6061 tgtggcaccc atactcccga tcagtccact gcttatatgc agggcacggc gcaggctgat
     6121 tctgcctttt atcttcagca gatgcagcaa agctctgatg ataccaggat caactggcaa
     6181 ttactcgcca ttcgtgcact ggtgaaagaa ggtaaaaccg ggcaggcggt tgagttgttt
     6241 aaccaactac cgcaagaact gaacgattct cagcgtcgcg agaaaacact gctggcggta
     6301 gagattaaac tggcgcagaa agattttgct ggcgcgcaaa acttgctggc gaaaatcaca
     6361 cctgccgatt tagaacaaaa ccagcaagcg cgttactggc aggcaaaaat cgatgccagc
     6421 caggggcgtc cttccattga tttactgcgc gcgttaattg ctcaggaacc gctgctcggc
     6481 gcgaaagaaa aacagcagaa tattgatgcc acctggcagg cgctctcctc catgactcag
     6541 gaacaggcga atacgctggt gatcaacgcc gacgaaaata ttctgcaagg ctggctggat
     6601 ctgcagcgcg tctggtttga taaccgtaac gatcccgaca tgatgaaagc cgggatcgcc
     6661 gactggcaaa aacgttatcc gaacaatccg ggcgcgaaaa tgctgccaac gcagttggtt
     6721 aacgtaaaag cgtttaaacc agcctcgacc aacaaaatcg ccctgctgtt gccgctgaat
     6781 ggccaggcag cggtatttgg tcgcactatt cagcaaggct ttgaagcggc gaaaaatatc
     6841 ggcactcagc cagtggcggc tcaggtagct gccgcacctg ccgcagacgt agctgaacaa
     6901 cctcagccgc aaaccgtgga tggcgttgcc agcccggcac aagcctcggt tagcgatctg
     6961 accggtgaac agcctgcggc acagccagcg cctgtaagca ccccggcagc aaccaccaca
     7021 gcggtaagcg cgccagcgaa tccatccgca gaactgaaaa tctacgatac ctcatcacaa
     7081 ccacttagcc agatcttaag ccaggttcag caggatggcg cgagtattgt ggttggtccg
     7141 ttgctgaaaa ataacgttga agagttgctt aagagcaaca ccccgctgaa cgtgctggca
     7201 ctgaaccagc cggagaatat cgaaaaccgc gtcaatattt gttacttcgc gctttcaccg
     7261 gaagacgaag cgcgcgatgc agcgcgtcat attcgtgacc agggtaaaca agcgccgctg
     7321 gtgctgatcc cacgcagttc attgggcgat cgcgtagcca atgcgtttgc gcaggagtgg
     7381 cagaaactgg gcggcggcac cgttctgcaa caaaaatttg gttccaccag cgaattacgc
     7441 gcgggtgtta acggcggttc tggtatcgct ttaacgggta ccccgattac tcccagagcg
     7501 acaaccgact ccggcatgac gaccaacaat ccaacgctgc aaaccacgcc aaccgatgac
     7561 cagttcacca ataatggcgg tcgtgtcgat gcggtgtaca ttgtggcaac gccgggtgaa
     7621 atcgctttta tcaaaccgat gatcgccatg cgtaacggta gccagagcgg tgcaacgctg
     7681 tacgccagct cccgcagtgc gcaagggacc gctggcccgg atttccgact ggagatggaa
     7741 ggcttgcagt acagcgaaat cccgatgctg gcaggcggta atctaccgtt aatgcagcag
     7801 gcactcagcg cggtgaataa cgattattca ctggctcgca tgtatgcgat gggcgtcgat
     7861 gcctggtcgc tggcaaatca tttctcacaa atgcgccagg ttcagggttt tgaaatcaac
     7921 ggtaataccg gaagcctgac ggcaaacccg gattgcgtga ttaacaggaa gttatcatgg
     7981 ctacagtacc aacaaggtca ggtagtcccc gccagttaac caccaaacag accggcgatg
     8041 cgtgggaagt acaagcgcgt cgctggctgg aaggcaaagg actgcgattt gtcgccgcta
     8101 acgtgaacga gcgtggcggc gagatcgatc tgataatgcg tgaaggccgg accaccgttt
     8161 ttgtcgaggt acgctatcgc cgctctgcgc tttatggcgg cgcggcagcc agtgtgaccc
     8221 gcagcaaaca acacaaatta ttacagactg cccgcttgtg gctcgcgcgt cataatggga
     8281 gttttgatac tgtggattgc cggttcgatg tggtagcctt caccgggaat gaggttgagt
     8341 ggattaagga tgcctttaat gaccactcat aattaaggtt taaggattag cgtgcaagaa
     8401 agaattaaag cttgcttcac tgaaagcatt caaactcaaa ttgcggcggc agaggcgctt
     8461 ccggatgcca tctcccgtgc agccatgacg ctggttcagt ctctgctcaa tggcaacaaa
     8521 atcctctgtt gtggtaatgg aacttccgct gccaatgcac agcattttgc tgccagcatg
     8581 atcaaccgtt tcgaaacgga gcggcccagc ttacctgcca ttgcactaaa tactgataat
     8641 gttgtcttaa cggcgattgc caacgatcgc ttacatgatg aagtgtatgc aaaacaggtg
     8701 cgggcgctgg gtcatgcggg agatgtattg ttagccattt ccacccgtgg caacagccgc
     8761 gatattgtaa aagcggtaga agctgccgtt acgcgtgata tgactattgt ggcattgacc
     8821 ggctatgacg gcggcgaact tgcaggtttg ttagggccac aagatgtgga gatccgcatt
     8881 ccttcgcatc gtagtgctcg cattcaggaa atgcatatgc tgacggtaaa ttgcctgtgc
     8941 gatctgatcg ataacacgct tttccctcac caggatgttt aaggagaata catgaaggca
     9001 ttatcgccaa tcgcagtcct tatttccgcg ctgctgttgc aaggttgtgt tgccgctgcc
     9061 gtagtgggta ccgctgctgt gggtaccaaa gccgcaactg acccacgcag tgtcggcacc
     9121 caggtggacg atggtaccct ggaagtgcgc gtgaacagcg cattgtcgaa agacgaacag
     9181 attaagaaag aagcccgtat taatgtcacg gcgtatcagg gcaaagtgct gctggttggg
     9241 cagtcaccaa acgctgaact ttcggctcgc gccaaacaga ttgctatggg cgtagacggt
     9301 gccaacgaag tgtataacga gattcgtcag ggccagccga ttggtctggg cgaagcgtct
     9361 aacgatacgt ggatcaccac caaagtgcgt tcgcagctct taaccagcga cctggtgaaa
     9421 tcgtccaacg tgaaagtgac caccgaaaac ggtgaagtgt tcctgatggg gctggtgact
     9481 gaacgtgaag cgaaagcggc ggcagatatt gccagccggg tgagcggcgt aaagcgggta
     9541 accacagcgt ttacgtttat taaatagcag ccccttgtaa tgcctgatgc gacgcttgcc
     9601 gcgtcttatc aggcctacaa cgatgcaaac cgtaggtcgg ataaggcgtt tacgccgcat
     9661 ccgacatgtt ttccctcaaa tcagaacaac agcgccagcc cgccgacaat cacaccagac
     9721 actgccacca tcgcccctgt cagccataag gctttcgctg ggaacgcttt gcgcagcata
     9781 atcagtgacg gcaaactcac cgccgggagc gtcatcaaca atgccagcgc cggagcagtt
     9841 cccatacctg ccagcatcat cgtttgtaca atcggaattt ctgctgccgt gggaatgaca
     9901 aacaagcatc ctgctaccgc catcgccatc acccacatca ggctgttatc gacagcacca
     9961 tcggcatggg ggaataacca gacgcgagcg gcacccaaca ccagtactgc aaggatgtaa
    10021 accgggatcg tactccagaa aagcgtccat agcgccctgc cccagcggct aaaaaaaccg
    10081 ccctgtgctt ccggtatgtc aatttcgacc ggtgcctgcg tttgcggtgt ttcacgcacc
    10141 catttttgca ccagcgtcgc aatcagcaac accatcacca gcccggccac cagacgaatc
    10201 gccgcaaaac cccagccgag gacaaacccc ataaacacca gcgtcgccgg gtttaacacc
    10261 ggattgccca tccagaatgc cagcgcaccg cccatcgaca cctgttggcg acgcattccc
    10321 gccgcgaccg gagccgcaca gcaggtacac atcatgcccg gcagcgaaaa cagcgttccc
    10381 agcagcgtgc cgcgaaagcg cgattgccca agcgtacgca acaaccagtc acgcgggatc
    10441 agcacctgaa tcaacgaacc gaggatcacc cccagcactg ccgctttcca taccgcgagg
    10501 aaatagagca tcgcgtaatc caacgccgcc tgccacggat tagcatccgc ctgcgcaagg
    10561 atagatttac cgatactgtg ggtttcggca gcagtaaacg ctttgccgta gtaaggttgc
    10621 catttcacat accagagacc ggcaatgaca acgagaaaga aaagcgcggg tttccaccac
    10681 tgaatgggcg ttgccgcctg agatgaagac tgaccagtca tagcattccc caggaataag
    10741 ttatgataat tgagcgcgtg aatattacgc tcactatcaa ttcttgggaa taattattca
    10801 gctcttttgc gtaattctga agagcttaaa atcgtcacac cttcatgccc gggccgcatc
    10861 gcctctgcca gcatgacccg cgccacgtct ctcgcatcaa tggatttcca gttaccgggt
    10921 aataaacgga acagcggcgc aaaaagcgtt tcgttcatcc gctgtttgct acgatcgccc
    10981 agtaacatcg acgggcgagc aatggtcaat ttcggccagt tctgggcgat taatgcttct
    11041 tccatctccc ctttgacgcg gttatagaaa aacggcgagt gggcattggc acccatagca
    11101 ctgaccacca gcatatgctg cgcgcccagt cgccgcccgg ttaatgcggt atccactacc
    11161 agcgtgtaat cggcatgaat aaacgcctct ttgctccccg cttctcgccg cgtggtgccg
    11221 agacaacaaa acacaatgtc gatgggatcg gtgacctgcg ccagcgcgtc agtcagttgc
    11281 ggatcatggg gattaaacac cccgggcata tcgcccaacg gacgtcgcgt cggcgcggct
    11341 atggcgttaa ctttcggttc gttaatcaac atccgcagca ggtgaccgcc caccagcccc
    11401 gttgcgcctg taatcagtac ctgactcatc ttcgctcctt tacagaattg tctgccttgc
    11461 gctccacggc tcatgcacca ggcttaatag accatgaggt aattatcccc gattgtggga
    11521 aattcgcctc atccaatgca acaacgcgga ggaagcatga gtaagaaaat tgccgtttta
    11581 atcactgatg aatttgaaga ttcagaattt acttcacccg cagacgagtt ccgtaaagcc
    11641 ggacacgaag tgattaccat tgaaaaacaa gcgggtaaaa cggtgaaagg caaaaaagga
    11701 gaagccagcg tgaccatcga taaatccatc gatgaagtga cgccagcgga gtttgatgcc
    11761 ctgctgctac cgggcggcca ttcaccggat tatctgcgtg gcgacaaccg ttttgtcacc
    11821 tttacccgtg attttgtgaa tagcggcaaa cctgttttcg ccatctgtca cggtccgcag
    11881 ctgctgatca gcgccgatgt gattcggggg cgcaaactga ccgcggttaa accgatcatt
    11941 attgatgtta aaaacgcggg cgcggaattt tacgatcagg aagtcgtggt cgataaagat
    12001 cagctggtta ccagccggac accggacgat ctgccagcgt ttaaccgcga ggcgttacgc
    12061 ctgctcggtg cctgagccgc gtagccaaat cattttttta ccaaagccca gcgtgttgtc
    12121 ggtgaatttg atttcatcga gccggatttc ccacaccggg gccgacagca ttctggcaac
    12181 cggaaagcga cgattgtacg ccttgcgcgc gaggtcgctt tcctcacctt ccagcctgcg
    12241 gatctcacct ttaaactgca cgccgcgaat taacgctacc gttttcggct gaccgtttac
    12301 cgttccggca accgcagcct gcggcccact catctgcgcg tggcgcgttt tttcttccgt
    12361 caaaatgtag aaggcgactt tctgcgcatc aaacagataa aaagcattag cgcaccagag
    12421 ttccccttcc tgctgcacac accaggtgac aacatgttgt tttgccaacc aacggctgat
    12481 ggtgatgagt gtttccattg ctgttctctt ttatactgtg ggcctgacct taacatgcga
    12541 cgcagacgat gacaccctgg tttctttact tgatccgtac cgccgacaat aagctttata
    12601 ccgggatcac cacggatgtc gaacgccgct atcagcagca ccaaagcggc aaaggggcga
    12661 aagcactgcg cgggaaagga gaactgacgc tggcgttttc tgcgccagtg ggcgatcgtt
    12721 cgctggcgtt acgggcggaa tatcgcgtta agcaactgac caaacggcaa aaagagcgtc
    12781 tggtagcgga aggcgcaggg tttgcagagc tgttaagcag tctgcaaacc ccggagatta
    12841 aaagcgattg aaatgctcgt gatactcaac cagaccagtt acgccattca acgcgtcatc
    12901 cgctaaacga tgtacctgga aggcgctttc agtgcctggc cagcggcaac gcaggtcgtg
    12961 atgtgccgcc agttcaaagc caaaacggct gtacagcgcc ggatcgccca gcgtcaccac
    13021 tgcggcatag ccgaactcat taagcgaatc gagtccttca tagaccagtt ggcgtgccag
    13081 cccctgtcca cggtattttt catcaaccgc caacggtgcc atgccgaccc attgcaggtc
    13141 ttcgccctgc acatcaaccg gactaaatgc cacatagcca atgacctgac cttcgtcatc
    13201 tgtcgccacc agccccagcg tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc
    13261 ttccgcatca ctttcgaatg agcgacgtag cagggcatca atacccggcg catcaatggg
    13321 aatttctact cgaattagca tggttcacct accgatgtct gtttggtttc aggcgcggtt
    13381 ttcattcccg cctcaacaaa atccgccagt tgcagcagca tcatgcgtaa ggctttcggc
    13441 atttgctcca gttcaatggc gtccatcagg tttttcacat acagccccag ctccgtatcg
    13501 ccttcaatca ccagccgacg ctggaagaag agcgtatccg gatcttgttt acgcgccgca
    13561 atcatcagca gatcgctggc gtcggcacta aaactcacat cagcttgcgc gttctggctg
    13621 accaccagtt tgccattcac caccgaggta aaccattgca ggtcaatatc acgcacatga
    13681 atacttaacc agcggccttc aagaaactcc agctcgccat catccagcgc ctgacggaat
    13741 tgccagctta agacctgctc aagaacctgg cgttttagcg caaatggcgt cagttttacc
    13801 ggtacactca acagagatgg ccccaaatgc acaatacggg aacgcagttt atccaacacg
    13861 agttttactc cctgtttcaa caatcatcct attttgccat atcagaaaaa taacatagcg
    13921 gtataaatca acaattccat atgaaattgc tgctaccacc aatacaactt taactgcctt
    13981 aaatcaaaaa ttgtcgcagc aaggttaact aaaatcccag ttcgttaaca tttttgcgtt
    14041 ttgatagcgc aaccttcagg aaaaattatg gagctgctct gccctgccgg aaatctcccg
    14101 gcgcttaagg cggccatcga aaacggcgca gatgctgttt atatcgggct aaaagatgat
    14161 accaatgccc gtcacttcgc cggccttaac tttaccgaga aaaaattgca ggaagcggtg
    14221 agttttgtcc atcaacatcg ccgcaaactt cacatcgcga ttaacacttt tgcgcatccg
    14281 gacggttacg cccgttggca gcgtgccgtg gatatggcgg cgcagctggg tgccgacgcg
    14341 ctgatcctcg ccgacctcgc catgctagag tacgccgccg agcgttaccc gcatattgag
    14401 cgtcatgtgt cagtgcaggc ttcggcgacc aatgaagagg cgattaactt ttatcatcgc
    14461 cattttgacg ttgctcgcgt ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacaa
    14521 ctggcacggg tcacacctgt accactggaa gtctttgctt tcggcagcct gtgcattatg
    14581 tcggaaggtc gttgctatct gtcgtcgtat ctgacgggtg agtcgcccaa caccgtaggc
    14641 gcgtgttctc cggcccgttt cgtgcgctgg caacaaacgc cgcaggggct ggaatcccgc
    14701 ctgaacgaag tgctgatcga ccgttatcag gacggcgaaa acgcaggtta tccgacgcta
    14761 tgtaaagggc gttatctggt ggacggcgag cgctatcacg cgctggaaga accaaccagt
    14821 ctcaataccc tggaactgct gccggagtta atggcggcga atattgcttc ggtgaaaatt
    14881 gaaggccgcc agcgtagccc ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct
    14941 atcgaccgtt gtaaggccga tccgcaaaac ttcataccgc aaagcgcgtg gatggagacg
    15001 ctcgggtcga tgtccgaagg cacgcagacc acccttggcg cgtatcaccg taaatggcag
    15061 tgagaagagc aatgaaatat tccttagggc cagtgctgtg gtactggcca aaagagacgc
    15121 tggaagattt ttatcagcag gccgccacca gcagcgccga cgtgatttat cttggtgaag
    15181 cggtatgcag caagcgccgg gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc
    15241 tcgccggaag tggcaagcag attgtgctct ccacgctggc gctggtgcag gcatcctctg
    15301 aactgggcga actgaaacgc tatgttgaga acggtgagtt tctgattgaa gccagcgatc
    15361 tcggcgtggt gaatatgtgc gctgaacgca aactgccgtt cgtcgccggg cacgcgctga
    15421 actgctacaa cgcggtgacg ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta
    15481 tgccggtgga gctttcccgc gactggctgg tgaatctgct taatcagtgc gatgagctgg
    15541 gcattcgcaa ccagtttgaa gtggaagtcc tgagctacgg tcatctaccg ctggcctact
    15601 ccgcccgctg ctttaccgcg cgttcggaag accgcccgaa agatgagtgt gaaacctgct
    15661 gcattaagta tccgaacggg cgcaacgtgc tgtcgcagga aaaccaacaa gtgtttgtac
    15721 tcaatggcat tcagaccatg agcggctacg tttacaacct cggtaacgag ctggcatcca
    15781 tgcaggggct ggttgatgtg gtccgcctgt caccgcaggg tactgacact ttcgcgatgc
    15841 tcgacgcctt ccgcgctaat gaaaatggcg ctgcgccact accgttgacg gcaaacagcg
    15901 actgtaacgg ctactggcgg cggctggccg ggctggagct gcaagcctaa gtaaatatgc
    15961 tcgtcatact tcaagttgca tgtgctgcat ctgcattcgt tcaccccagt cacttactta
    16021 tgtaagctcc tggggattca ctctcttgtc gccttcctgc aactcgaatt atttagagca
    16081 cagctcactt tgttaacaac tttaactact ctttaatgca gtattaaaga ttaatcggta
    16141 acaaagtgag ctgttatgac tgataaaacc attgcgtttt cgctactcga tctggccccc
    16201 attcccgaag gttcttcagc gcgagaagca ttctcccact ctctcgatct cgcccgtctg
    16261 gctgaaaagc gcggctatca tcgctactgg ctggcagaac accacaatat gactggcatt
    16321 gccagtgctg ccacgtcggt attgattggc tatctggcgg cgaataccac cacgctgcat
    16381 ctggggtctg gcggcgtgat gttgcctaac cactcaccgt tggtcattgc cgaacagttc
    16441 ggcacgctca atacactcta tccggggcga atcgatttgg ggctgggtcg tgcgccaggt
    16501 agtgaccaac ggacaatgat ggcgctgcgt cgccatatga gcggcgatat tgataatttc
    16561 ccccgcgatg tcgcggagct ggtggactgg tttgacgccc gcgatcccaa tccgcatgtg
    16621 cgcccggtac caggctatgg cgagaaaatc cccgtgtggt tgttaggctc cagcctttac
    16681 agcgcgcaac tggcggcgca gcttggtctg ccgttcgcgt ttgcctcaca cttcgcgccg
    16741 gatatgttgt tccaggcgct gcatctttat cgcagcaact tcaaaccgtc ggcacgactg
    16801 gaaaaaccat acgcgatggt gtgcatcaat attatcgccg ccgatagcaa ccgcgacgct
    16861 gagtttctgt ttacctcaat gcagcaagcc tttgtgaagt tgcgccgtgg cgaaaccggg
    16921 caactgccgc caccgattca aaatatggat cagttctggt cgccgtccga acagtatggt
    16981 gtgcagcagg cgctgagtat gtcgttggta ggtgataaag cgaaagtgcg tcatggcttg
    17041 cagtcgatcc tgcgcgaaac cgacgccgat gagattatgg tcaacgggca gattttcgac
    17101 caccaggcgc ggctgcattc gtttgagctg gcgatggatg ttaaggaaga gttgttggga
    17161 tagtgtgtct taacgcggga agccttatcc gagctggcaa cgctgtcc
//
LOCUS       DAZGOB010000106        17094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGOB010000106 DAZGOB010000000
VERSION     DAZGOB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.106"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            97..1236
                     /gene="arnB"
                     /locus_tag="ACHLIF_003671"
     CDS             97..1236
                     /gene="arnB"
                     /locus_tag="ACHLIF_003671"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO9607611.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTPMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1240..2208
                     /gene="arnC"
                     /locus_tag="ACHLIF_003672"
     CDS             1240..2208
                     /gene="arnC"
                     /locus_tag="ACHLIF_003672"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO9607612.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2208..4190
                     /gene="arnA"
                     /locus_tag="ACHLIF_003673"
     CDS             2208..4190
                     /gene="arnA"
                     /locus_tag="ACHLIF_003673"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO9607613.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4187..5077
                     /gene="arnD"
                     /locus_tag="ACHLIF_003674"
     CDS             4187..5077
                     /gene="arnD"
                     /locus_tag="ACHLIF_003674"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO9607614.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5077..6729
                     /gene="arnT"
                     /locus_tag="ACHLIF_003675"
     CDS             5077..6729
                     /gene="arnT"
                     /locus_tag="ACHLIF_003675"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO9607615.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6726..7061
                     /gene="arnE"
                     /locus_tag="ACHLIF_003676"
     CDS             6726..7061
                     /gene="arnE"
                     /locus_tag="ACHLIF_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO9607616.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7061..7447
                     /gene="arnF"
                     /locus_tag="ACHLIF_003677"
     CDS             7061..7447
                     /gene="arnF"
                     /locus_tag="ACHLIF_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO9607617.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7441..7707)
                     /gene="pmrD"
                     /locus_tag="ACHLIF_003678"
     CDS             complement(7441..7707)
                     /gene="pmrD"
                     /locus_tag="ACHLIF_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO9607618.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7817..9172)
                     /gene="menE"
                     /locus_tag="ACHLIF_003679"
     CDS             complement(7817..9172)
                     /gene="menE"
                     /locus_tag="ACHLIF_003679"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO9607619.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9169..10131)
                     /gene="menC"
                     /locus_tag="ACHLIF_003680"
     CDS             complement(9169..10131)
                     /gene="menC"
                     /locus_tag="ACHLIF_003680"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO9607620.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10131..10988)
                     /gene="menB"
                     /locus_tag="ACHLIF_003681"
     CDS             complement(10131..10988)
                     /gene="menB"
                     /locus_tag="ACHLIF_003681"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO9607621.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11003..11761)
                     /gene="menH"
                     /locus_tag="ACHLIF_003682"
     CDS             complement(11003..11761)
                     /gene="menH"
                     /locus_tag="ACHLIF_003682"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO9607622.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11758..13428)
                     /gene="menD"
                     /locus_tag="ACHLIF_003683"
     CDS             complement(11758..13428)
                     /gene="menD"
                     /locus_tag="ACHLIF_003683"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO9607623.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13517..14812)
                     /gene="menF"
                     /locus_tag="ACHLIF_003684"
     CDS             complement(13517..14812)
                     /gene="menF"
                     /locus_tag="ACHLIF_003684"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO9607624.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14891..15196)
                     /gene="elaB"
                     /locus_tag="ACHLIF_003685"
     CDS             complement(14891..15196)
                     /gene="elaB"
                     /locus_tag="ACHLIF_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO9607625.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15251..15712)
                     /locus_tag="ACHLIF_003686"
     CDS             complement(15251..15712)
                     /locus_tag="ACHLIF_003686"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9607626.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15777..16694
                     /gene="rbn"
                     /locus_tag="ACHLIF_003687"
     CDS             15777..16694
                     /gene="rbn"
                     /locus_tag="ACHLIF_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO9607627.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16882..>17094
                     /locus_tag="ACHLIF_003688"
     CDS             16882..>17094
                     /locus_tag="ACHLIF_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO9607628.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHI"
BASE COUNT         3972 a         4625 c         4354 g         4143 t
ORIGIN      
        1 tctatcctta aaatttcgct ccaaatggca aaatatacac aacactcttt atagcaaata
       61 taagtggaca ggtattcaat ggcggaagga aaagcaatgt cagaattttt gcctttttcg
      121 cgaccagcaa tgggcgtgga ggaactcgct gcagttaaag aggttctcga atccggttgg
      181 atcacaaccg gtccgaagaa tcaggcgctt gagcaagcct tttgccagtt gacgggaaat
      241 cagcatgcca tcgcggtcag ttcagccacc gccggaatgc atatcacgct aatggcgttg
      301 gaaattggca agggcgatga agtgattacg ccttccctga cctgggtttc aaccctcaat
      361 atgatttcct tgttgggtgc aacgccggta atggtggatg tcgaccgcga tacgccgatg
      421 gtcacgcctg aagcgatcga gtcagccata acgccacgca ctaaagccat cattccggtg
      481 cattatgccg gtgcgccagc agatattgac gccattcgcg ccattggcga acgttacggc
      541 atcgcagtta tcgaagatgc tgcccatgcc gtcggtacgt attacaaagg gcgacatatt
      601 ggcgcaaaag gtaccactat tttttcattt catgccatta aaaatattac ctgtgcggaa
      661 ggtggcctga ttgtaactga taatgaaaac cttgcccgcc agctacggat gctgaaattt
      721 cacggtctgg gtgtcgatgc ctatgacaga caaacctggg gccgtgcacc gcaggctgaa
      781 gtcttaacac cgggctataa gtacaatctg accgatatta acgccgcaat tgccctgaca
      841 cagttagcaa aattagagca cctcaacacc cgtcggcgcg aaattgccca gcaatatcag
      901 caagcactgg cagctctccc ctttcagcca ttaagccttc ccgcctggcc gcacgttcac
      961 gcctggcatc tgtttattat tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg
     1021 ttgatggaag cgttaaaaga aagaggcatt ggtaccgggt tacatttccg cgccgctcac
     1081 acacaaaaat attatcgcga gcgttttccc acgctgtcgt taccgaatac cgaatggaat
     1141 agcgaacgca tctgttcttt gccgctgttc ccggatatga ctaccgccga tgccgaccgt
     1201 gtcatcacag cccttcagca actcgcagga caataagcca tgtttgaaat ccaccctgtt
     1261 aagaaagtct cggtggttat tcccgtttat aacgagcagg aaagcttacc ggaattaatc
     1321 aggcgcacca ccacagcctg tgaatcgttg gggaaagagt atgagatcct gctgattgat
     1381 gacggcagta gcgataattc cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc
     1441 catattgtgt ctattttgct taaccgcaat tacgggcaac attcagcgat tatggcggga
     1501 ttcagtcacg ttaccggcga cttaattatt acccttgatg ccgatctcca gaatccgcca
     1561 gaagaaatcc cccgcctggt ggcaaaagcc gatgaaggtt acgacgtggt agggactgta
     1621 cgccagaacc gccaggacag ctggtttcgt aaaacagctt cgaagatgat taaccggctt
     1681 attcagcgca ccaccggcaa agcgatgggt gactacggtt gtatgctgcg cgcctatcgc
     1741 cgtcatattg tcgatgcgat gttgcactgc catgaacgta gcacctttat cccgattctg
     1801 gcgaatatct tcgcccgccg tgccattgaa attccagtac atcattccga gcgtgagttt
     1861 ggtgaatcca aatacagttt tatgcgcctg attaatttga tgtacgacct ggtgacctgc
     1921 cttaccacaa cgccgctacg tatgctgagt ctgctcggca gcattattgc gattggaggt
     1981 tttagcattg cggtgctgct ggtgatttta cgcctgacct tcggaccaca atgggcggca
     2041 gaaggcgtct ttatgctatt tgccgtgctg tttactttta ttggcgctca gtttatcggc
     2101 atgggattac tcggtgaata tatcggcagg atctacaccg atgtccgcgc ccgcccccgc
     2161 tattttgttc agcaagttat ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg
     2221 tttttgccta ccacgatatg ggatgcctcg gtattgaagc tctgctggct gccggttacg
     2281 aaattagcgc catttttacc cataccgata atcccggtga aaaagccttt tatggttcgg
     2341 tggctcgtct ggcagcggaa agaggcattc cggtttatgc gccggatgac gttaatcatc
     2401 cgctgtgggt ggaacgcatt gcccaactgt cgccagatgt gattttctct ttttattatc
     2461 gccatcttat ttgcgacgaa attttgcagc tcgctcccgc aggtgcattt aatctgcatg
     2521 gttcgctgtt accaaaatat cgtggtcgcg cgccgctgaa ctgggtgctg gtcaacggtg
     2581 aaacggaaac tggcgttaca ttgcaccgaa tggtgaaacg tgccgatgcc ggggccattg
     2641 tggcgcaact gcgcattgcc attgcgccag acgatatcgc tattacgctg catcataaat
     2701 tgtgccatgc cgcgcgccag ctactggaac agacattacc cgccattaaa cacggtaata
     2761 ttctggaaat cgcccagcgc gaaaacgaag ccacctgttt tggtcgcaga acgccggatg
     2821 acagtttcct tgaatggcat aaaccggcat ccgtactgca caacatggta cgtgccgttg
     2881 ccgatccgtg gccgggtgcc ttcagctatg ttggcaatca gaaattcacc gtctggtcgt
     2941 cgcgtgttca tcctcatgcc agcaaagcac agccggggag cgtgatttct gttgcgccac
     3001 tgctgattgc ctgtggcgat ggcgcgctgg aaatcgtcac cggacaggcg ggcgacggca
     3061 ttactatgca gggctcgcaa ttagcgcaga cgctgggcct ggtgcaaggt tcacgcttga
     3121 atagccagcc tgcctgcacc gcccgacgcc gtacccgggt actcatcctc ggggtgaatg
     3181 gctttattgg caaccatctg acagaacgcc tgctgcgcga agatcattat gaagtttacg
     3241 gtctggatat tggcagtgat gcgataagcc gttttctgaa tcatccgcat tttcactttg
     3301 ttgaaggcga tatcagtatt cattccgaat ggattgagta tcacgtcaaa aaatgtgatg
     3361 tcgtcttgcc gctggtggcg atagccacgc cgattgaata tacccgcaac ccgctgcgcg
     3421 tatttgaact cgattttgaa gagaatctgc gcattatccg ctactgcgtg aagtaccgta
     3481 agcgaatcat cttcccgtca acttcagaag tttatgggat gtgtagcgat aaatacttcg
     3541 atgaggacca ttctaattta atcgtcggcc cggtgaataa accacgctgg atttattcgg
     3601 tatcaaaaca attacttgat cgggtgatct gggcctatgg cgaaaaagag ggtttacagt
     3661 tcaccctctt ccgcccgttt aactggatgg gaccgcgact ggataacctt aatgcggcac
     3721 gaatcggcag ctcccgcgct attacgcaac tcattctcaa tctggtagaa ggttcaccga
     3781 ttaagctgat tgatggcgga aaacaaaaac gctgctttac tgatattcgc gatggtatcg
     3841 aggcgttata ccgcattatc gaaaatgcgg gaaatcgctg cgacggtgaa attatcaaca
     3901 ttggcaatcc tgagaacgaa gcgagcattg aggaactggg cgagatgctg ctggcgagct
     3961 tcgaaaaaca tccgctgcgc catcatttcc caccgtttgc gggctttcgc gttgtcgaaa
     4021 gtagcagcta ctacggcaaa ggatatcagg acgtagagca tcgtaaaccg agcatccgca
     4081 atgcccaccg ctgcctggac tgggagccga aaattgatat gcaggaaacc atcgacgaaa
     4141 cgctggattt cttcctgcgc accgttgatc ttacggataa accatcatga ccaaagtagg
     4201 cttacgcatt gatgtcgata cctttcgtgg cacccgtgaa ggcgtgccgc gtctgctgga
     4261 aatcttgagt aagcataata ttcaggccag catttttttc agcgtcggcc cggacaatat
     4321 gggccgccat ctctggcgac tggtgaagcc acagtttttg tggaagatgc tgcgctcaaa
     4381 tgcggcatcg ctttatggct gggatatttt actggcaggt acggcctggc caggtaaaga
     4441 gattggtcat gccaatgccg atatcattcg tgaagcggct aaacatcacg aagtcggcct
     4501 gcacgcctgg gatcaccatg cctggcaagc ccgtagcggt aactgggatc ggcaaacaat
     4561 gatcgacgat attgcacgcg gtcttcgcac tctggaagag attatcggtc aaccggtaac
     4621 ctgttctgcc gctgcgggct ggcgtgccga ccagaaggtg atcgaagcaa aagaagcgtt
     4681 ccatttgcgc tacaacagcg attgtcgtgg ggccatgccg ttccgtccat tgctcgaatc
     4741 aggaaaccct ggcactgcgc aaattccggt gaccttaccc acctgggatg aagtgattgg
     4801 tcgggatgtg aaagcagaag attttaacgg ttggttactc aaccgcatcc tgcgagataa
     4861 aggcacgccg gtttatacca ttcatgcaga agttgaaggc tgcgcttatc agcataattt
     4921 tgtggatctc ctcaaacgcg cagctcagga aggagtgaca ttttgccctt taagcgaact
     4981 gttatcagag acgttgccgc tcggacaagt tgttcgcgga aatattgccg gacgtgaagg
     5041 ctggctgggt tgccaacaaa ttgcgggtag tcgctgatga aatcggtacg ttaccttatc
     5101 ggcctcttcg cgtttattgc ctgctattac ctgttaccga tcagcacgcg tctgctctgg
     5161 caacccgatg aaacgcgtta tgcggaaatc agtcgagaaa tgctggcatc cggcgactgg
     5221 attgtgcccc atctgttagg gctacgttat ttcgaaaaac ccattgccgg atactggatt
     5281 aacagcattg ggcaatggct atttggcgcg aataactttg gtgtgcgggc aggcgttatc
     5341 tttgcgaccc tgttaactgc cgcgctggtg acctggttta ctctgcgctt atggcgcgat
     5401 aaacgtctgg ctctactcgc cacagtaatt tatctctcat tgtttattgt ctatgccatc
     5461 ggcacttatg ccgtgctcga tccgtttatt gcattctggc tggtggcggg aatgtgcagc
     5521 ttctggctgg caatgcaggc acagacgtgg aaaggcaaaa gcgcaggatt tttactgctg
     5581 ggaatcacct gcggcatggg ggtgatgacc aaaggttttc tcgcccttgc cgtgccggta
     5641 ttaagcgtgc tgccatgggt agcgacgcaa aaacgctgga aagatctctt tatttacggc
     5701 tggctggcgg ttatcagttg cgtactgacg gttctccctt ggggactggc gatagcgcag
     5761 cgggagcctg acttctggca ctattttttc tgggttgagc atattcaacg ctttgcactg
     5821 gatgatgccc aacatagagc tccgttctgg tactacgtgc cggtcatcat tgccggtagc
     5881 ctgccgtggc tgggattact ccccggtgca ctgtacacag gctggaaaaa ccgcaagcat
     5941 tccgcaaccg tctatttgtt gagctggacg ataatgccgc tgctgttttt ctccgtcgct
     6001 aaaggtaaat tgcccaccta tattctttcc tgctttgcat ctctggcaat gctgatggcg
     6061 cattacgctt tgctggcagc aaaaaataat cctctggcgc tgcggattaa tggctggatt
     6121 aacatcgctt ttggcgtcac tggcattatt gccacatttg tggtctcccc gtggggacca
     6181 atgaacacgc cggtgtggca aaccttcgag agctataaag tcttttgtgc ctggtcgatt
     6241 ttttcgctat gggcattttt cggctggtac accttaacaa acgtcgaaaa gacctggcct
     6301 tttgccgcgc tttgcccgct ggggctggcg ttgctggtag gattttcaat tcctgacaga
     6361 gttatggaag gaaaacatcc gcaatttttt gtcgagatga cacaagaatc acttcagcca
     6421 agccgctata ttcttactga tagcgtcggt gttgccgcag gtctggcatg gagcctgcaa
     6481 cgcgatgaca tcatcatgta tcgccagaca ggtgagttga aatacggcct taattatccg
     6541 gatgcgaaag ggagatttgt cagcggtgat gagttcgcaa actggcttaa tcaacatcgt
     6601 caggagggga ttattactct cgtgctttcg gttgaccgcg atgaagatat caacagtctc
     6661 gccattccgc ccgcagatgc catcgatcgt caggagcgtc tggtgctgat tcagtatcgt
     6721 cccaaatgat ctggctaaca ttagtctttg ccagcttgct tagcgttgcc gggcagttgt
     6781 gtcagaaaca ggcaacctgc tttgtggcga taaacaaacg gcgcaaacat atcgtgctgt
     6841 ggctgggact ggcgctggct tgtcttggtc ttgccatggt gctctggctg ctggtcttgc
     6901 agaacgtacc ggtaggcatt gcctacccga tgttaagtct gaattttgtc tgggtgacgc
     6961 tggctgcagt aaaactgtgg cacgaaccgg tatcgccgcg tcactggtgt ggggtggcgt
     7021 tcattattgg tggcattgtg atcctcggga gtacggtgta atgggcctga tatggggatt
     7081 attcagcgtc attattgcct cagttgcgca attaagcctg ggttttgcgg caagtcatct
     7141 gccgccgatg acgcacttat gggattttat cgcggctctg ttagcttttg gcctggatgc
     7201 ccggatactg ttactcgggc tgctgggtta tctgctttcc gttttttgtt ggtataaaac
     7261 gctacataaa cttgcgctaa gcaaagccta tgccttgctc agtatgagtt atgtgctggt
     7321 atggattgct tcaatggtac ttcccggctg ggaagggact ttttcgctga aagcactact
     7381 gggagtagcc tgtattatga gcgggttgat gctgattttc ctgcccacga caaaacaacg
     7441 ttactgagtt ttccctgccg ctgtacactg ccgttcccac tcatcggggc tataactgct
     7501 tgccgagagg actttcaccg tatgcagctt ttcacgatta atacaataaa gcgcattctg
     7561 caaaggcgag agtaaatccc ccactttcac ggcgaagtca ctcttcactt cggcgatcat
     7621 ttttatcgcg ccgccagcat cgcagagcat aagtacatgt ctgttatctt gtttattgca
     7681 gcacgatttt ttgaccagcc attccattgc attatcctgt ttgctaagag ttttcacatc
     7741 aatttacaga ttaacagata aactctttat caacggcaat ggaatggcag aaatgagaag
     7801 tgaaacggag ggttaattat tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa
     7861 ttttaatacc gccgtttttc agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa
     7921 aacgagccag cttatctttc acccattcgc caagatcaac ggtttgctgg tcatactcca
     7981 ccaccgctac cggccgatga ccaaactcct tgtcggcaac gggaacgata aacacctgca
     8041 aaaccgcagg atgtgcagca ataacacgct cgacttcttc cggctgaata ccctctccgc
     8101 cactgaagaa tagattgtct aaacgtccga caatggtcag cttgccatta tgcatctcac
     8161 cgcgatcgcg cgtagcgtac cagccttcgt cattaaccag tgaaaccagt tgcccgttac
     8221 gccagtaacc ttctgccata ctggcagccc gcagccacac ttcattatta acgattttca
     8281 cttcccgacc cggcagcggc gaaccaacgt ctgccaggcc gtcggcttct ttcgcacaca
     8341 ccgtggaggc aaactcggtc agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg
     8401 cctgttccgt caactcgacc gggatagccg cgccgccaag taacaccgct ttcagggaaa
     8461 cggaactgcg gttaaccagc aaacgccaga gttgtgttgg caccagtgaa gcatgagtac
     8521 agcctgccag catttgctcc aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt
     8581 ataaccagcg ccacataatt ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc
     8641 agtcatcgtg atcgccaaac ggaatcaacg ataacacccc ttcggcactg gcaagatggg
     8701 cctgataagt atggacagcg gctttcggca acccggtaga acccgaggtc aacgtcattg
     8761 agcacagacg cgtcggctgc cacgtagcgg catgtgcgcc ttcaacccgc tgaatgtgca
     8821 gcgacgttaa cgccggaaac gtgttttccc catccggcac cagcgcaaat tgcagcgtca
     8881 gattgggcag caattcttca agcaacggtt gcggcagctg agggttcacg ggcaacaccc
     8941 gcgccccgca ttgcagtaac gccagccagg cgagcagcgt ttgcggcgta ttccacgccc
     9001 gcaacatcac gccgctgccc tcaacaacgc cctgcgccgc aaagcctgag gctaattcat
     9061 cgacgcgagc acaaagctcg cgccagttaa gttgctcgtc attaagacgt aaggcgatgg
     9121 cttctccccg cacttgccgc cagtgacgcc acggccagtc agagaagatc ataacaaccg
     9181 ctccagtgca tcaacttcca cgacaggcag cgtgctaccc ggccagcgac gtacctgctg
     9241 cgcctgcatc agatccagcg tgtccagccc tggaatggtg tccggcgtta accaggcggc
     9301 aatccgcgcc agttgcgtta agcctaagct cgattcaatg gaagaactga tcaccgccgt
     9361 cagccccagc gcgtgcgccg cctgtacctg ctcgcgtact ttctccagac tgcccgtgag
     9421 cgtgggtttg ataactaccg cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc
     9481 gcgcaagctt tcatcccagg caatggcaat gccggtttca cgggcaaacg ctcgcgaatc
     9541 atcgcgggtt ttgcacggct cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac
     9601 atatttggca aactgctggc ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa
     9661 atgcagatcc ggaattgcct ccagcaacag attaaccacc atgccgtcgc gcacggcttc
     9721 gtacaatccc actttgacct tcgccacttt ctcgcctggc atatcagcaa gtttgaggat
     9781 cagatcgtca ggatcgccat tacacagtgg tgccgcacgg taattggccg cctgcgggag
     9841 cgtatctgcc agctccgcca atgcacagct tacgccaaag gcaacggaag gcatctgcgg
     9901 tagctcgcaa tcgcctgcca gccagttatt tacccaggca agcagcacgc tttgcgcctc
     9961 ttcccaggtt tcctggctga agcccggcag tggggagatc tccccccacc cttcgcgctc
    10021 gccctcacgc aggcaaacgt acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac
    10081 cacccccgcg tccatgggga tctgccagcg gtatacctgc gcgctacgca ttacggattc
    10141 cgtttaaatt tgctgaaatc aggctgacgt ttctggttga aagcgttgcg accttcctga
    10201 ccttcttccg tcatgtagaa cagcatggtg gcgttgcccg ccagctcctg cagccccgcc
    10261 tgcccgtcac agtcggcgtt cagcgcagct ttcaggcagc gcagcgccat cgggctgttt
    10321 tgcagcattt cgcggcacca acggacggtt tctttttcca gatccgccag cggtaccacg
    10381 gtgttgacaa ggcccatatc cagcgcctgt tttgcgtcgt actgacggca caggaaccag
    10441 atttctcgcg cttttttctg cccgacgata cgagccatgt aggaagcgcc ccagccgccg
    10501 tcgaaggaac cgactttcgg gccagtctga ccgaagatgg cattatctgc tgcgatagtc
    10561 aggtcgcaca tcatgtgcag aacgtgacca ccgccgatgg agtagccagc caccatcgcg
    10621 acaaccggtt tcggacaggt acggatctga cgctggaagt ccagcacgtt caagtgatgt
    10681 acgccggaat catctttata gccgccgtaa tcaccacgca ctttctggtc accaccggag
    10741 cagaacgctt tatcgcctgc gccagtcaga atgatcacgc cgatattgtc gtcataacgc
    10801 gcatctgcca gtgcctggat catctctttt accgtcagag gacggaaggc attgcgcacc
    10861 tgcggacgat taatggtgat ttttgcgata ccgtcggtgg atttttcata acgaatgtcc
    10921 tcgaaacctt cggagcagtc gtgccattca accggtgcgt aaagcattgc ttcatcagga
    10981 taaatcatat tgtgtccttt agtcagaaac gcaagatctg cgccagactt gcgattacgc
    11041 cagcgggatt ttcccgatgc gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag
    11101 ccagttccgc cgccagggcg cggaatttgc tgtcacgttc accacataaa taataaaacg
    11161 caaatgtgcg ggcgctaagg ttagcacgta aatcaggctg gacggcgaga gaagtcgcct
    11221 ccagcatggc ggcaagggtt gcgccattat tgttgctgcg cagcgccacc agctcccggc
    11281 gttgatcgtc attgagcgag gcaaaaacag gctgttgata ccagtcggca aataccgccg
    11341 ttaacggttc tgtgcaaaaa cgctgcgccc attggcgatc ggaacgctga cgttccgcac
    11401 gttgttcagc attttgcagc cccggatgcc cgccttcgac aataacccca caaagccccg
    11461 ccagcccctg gcaagccgcc atcatcgcca cccgtccgcc aagcgagtac cccaccagcc
    11521 agaagtcaag gatgttgtaa ctaaccaagg ttttacgcag taagtcggtg acatcatcaa
    11581 atccatcgac gctaatcgcc gccgaaccac catgacctgg gagatcaaca tacaaccgtg
    11641 agtagtcggc aaacgcctcg cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt
    11701 gcaaaaacac cagccagggt aaacctggtt ttccgtgttt tgcctgcgcg tgcaggatca
    11761 taaatggctt acctgcgcca gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac
    11821 caccatttca atcaccgtgg tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag
    11881 ttcctgccag ttttgcggac gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa
    11941 atggacgttt tgcggcatca gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag
    12001 cgagaaaatt tgcccgccgt tgttgttcac cacaattaat accagcggcg cagaaacctg
    12061 acgcaataac gccagcgcgt tgagatcgta aagtgcggag agatcgccca caatcgccag
    12121 cgtcggtttg ccgcttgccc gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc
    12181 gctggcccct cggttgctgt acaccgggta acctgccgga agttgcgaaa gcgcatcaat
    12241 cagacgtacc accaggctgt taccaacaaa caattgcccc tgttcaggca gatagtcgct
    12301 gatgcgatgc gccagttgcg cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat
    12361 tgcctgttcc gccaggcgcg ggatttcaac gcaccagggc tggcgttttt ctgccggatg
    12421 cagctccagc cagtcggcaa tattggcaat taagcgacgt ccgcgatggt gtgccggatc
    12481 aagtcgccct tcaatgtcat caacaatcca gtactcttcg ggttcacagc ttgcctgcca
    12541 ttgcaggagc cgtttccccg tcaggctgct tcccagttgc accacaattt gcgcctgctg
    12601 cagctcgctg gtcgctttgg cattgcctaa ccaaagatcg gcacacggca gcggctgccc
    12661 ggtttgtgac agcacatcgc caatcagcgg ccagccaaga gtttgcgccc acagggcaac
    12721 ttttttgccc tcttccgcac tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca
    12781 gaagaaccag tcgcgctgtt tttcactttc cagacgaggc gcttcacgca gccacggttt
    12841 gtcgtcctgc caccagtcac ccagacgctg ttgccagcta agcccggtat cgtccatttc
    12901 gccatacagc ggttcagcaa acgggcagtt gatatggact ccccccgcat gaagcgtacc
    12961 gagagcgtgg tcgatggtag aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg
    13021 caatgaaata ctgtgcgtgg ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt
    13081 cgcgccgcag tcaattagct ccggcgggcg atcggcggtt aagagaatca gtttttctcc
    13141 ggttaacccg gcttcaatca gtgccggata gagatttgcc accgccgtgc cggaggtcac
    13201 aatcaccgcc accggctgct tgctgacttt cgccagcccc agcgccagat gccccagtcc
    13261 acgctcatcg aaatgggtgt ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa
    13321 cggtgtagaa cgcgagcctg gggcgataca gatgtgtctg acgccgtgac gcgttaatgc
    13381 ttccagaatg accgccgccc agcgtcggtt aaatgcgctt actgacatga gtttgtccgg
    13441 tatcaatatt gcggctaagt ataaggagct acaaaaatag attattgata tgaatcggta
    13501 atgatgcgac tcattactat tccatttgta ataaagtacg cagccctgcc gctttgttgt
    13561 cgatttcctg ccactcttgc tcggggtcgg aaccacggac aatgcccgcg ccagcatata
    13621 gccgcacgac attgccgctg atttttgctg aacgcaggga aacgcagaat tcgctttgtt
    13681 gtaatgagag atagcccgca gaaccggcgt accattctcg ggtgaacggt tcgtgacggg
    13741 cgataaactg tcgcgccaga tcgcgcggta agccagcaac tgctgccgtt ggctgcaact
    13801 gatgtaaaca gatcacatca tccgctttgt tgagtgaagt ccagatacag cggcgaagat
    13861 gctgcacttt acgcagacgc agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg
    13921 cctgtaatcg ttggcagata tcttccacca ccagcatgtt ctcgcgctgg tttttatcat
    13981 ccgccatcag ccactctcct aactgctgcg cctgcttatc atcaggatta tttgctactg
    14041 ttcccgccag cgcttcagta cgcagcgctt tgtcacgccg ccgccataac cgttccggtg
    14101 aagagccaag aaaagcattt tcgccatcaa aggccatgta aaaatggtag caattcagat
    14161 tcagtcgacg actggcagcc atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt
    14221 cagttgcccg agcgagcacc actttgtcga gctcaccttc ggcgatggtt ttcgttgcca
    14281 gttcgattaa ttgcgtccag cccgttttgt ccggccagtg ttgttctcgc gtggtggtta
    14341 aatgtaaccc aggcaagggc ttgatactca ccagtgtggc gataaattct tttgcctgaa
    14401 tcgcatcgtg ctgaagggag ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc
    14461 cacagcgtcg ccattccagg cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat
    14521 tcagccccca aatgcgtaag tcggcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct
    14581 ggtccaacga cgtaaaacgg gtaatcgcgc ccaggacggc tgcttcttca tcaccattac
    14641 gttgttgcca gtagaattgc gggtacgttt gctgactggc cagccagctc aaggcatcaa
    14701 aagcgtcttt gagagggaaa ggaatatcga taacccgaat gccgggtgtc gccggaatct
    14761 cttgcgacaa atggcgcagt agattttcca gcgccgtagt aagtgattgc acgcggacct
    14821 ctccctgtta aaagcctcac attatacggg gtactacaaa aaaaatgcag taccccggtg
    14881 tagggaggtt ttaacggcgt gccagcaaca gtcctagtac cagcccaacg gccgcgccca
    14941 caccaattcc ttgccagggt ttttcgtgga cgtagtcatc agcacgataa actgcctgct
    15001 tcgcccgata gtaataactg tctgaagcct ggctaacccg ttttttcaca tcatccagcg
    15061 ctttttctgc acgcgctttc agctcaacat atttctgatc ggcgggatcg ccagaggagc
    15121 ggagcacctc ttccagtgtt tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac
    15181 caaactgatt agacattctc gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa
    15241 gggttgttga ttacgcctga attacctcgc gtgccatccc aatgtgtggg ataccatctt
    15301 cttcatagac ctccgtcaca gggataaaac cgaaactctg gtagaagttt tgcagatgcg
    15361 cctgcgcccc gaggtaaaca ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt
    15421 tactcatcag ctgctgcccc actttttcgc cacgcaacgc ttcactgaca atcacccgac
    15481 ctataacgac cggctcaaga tcatcatcac ttttcagaat cctcgcatat gccaccagtt
    15541 catcattttt ccaaccgaga atatggcgat tatcccccgt caaatcatca ccatcgatat
    15601 cctgataagg gcaattctgc tcgacaacaa acaccgcgca acgcagttgt aataaggcat
    15661 aaagctggga aaccgataat tcagagtgat gcagatcttg ccattcaatc ataatgctct
    15721 ccttttagat gtatgcctta tactagccgg aatatttttt gaaacgtgat gaactcatgg
    15781 aattaatttt tttaggtact tcggccggtg tgccaacccg cacgcgcaat gtcacggcaa
    15841 tattgcttaa cctgcaacat ccgacccaga gcggtctttg gttgtttgac tgcggtgaag
    15901 gcacccagca tcagctactg cataccgcct ttaaccctgg aaaactggac aagattttta
    15961 tcagtcacct tcatggcgat catctttttg gtttacccgg cttgctgtgc agtcgttcta
    16021 tgtcaggcat tatccaaccc ttaacgattt atggtccgca aggtatccgt gaatttgtgg
    16081 aaaccgcgct gcggattagc ggctcatgga ccgattatcc gctggaaatt gtcgaaattg
    16141 gcgctggcga aattctcgat gatggcctgc gcaaagtaac cgcttatccg ctggaacacc
    16201 cactggaatg ttatggctat cgtattgaag aacatgataa accgggtgca ttaaatgccc
    16261 aggcattaaa agctgctggc gtgccgcctg gcccactgtt tcaggaatta aaagcgggca
    16321 aaacaatcac gctggaagat ggaaggcaga ttaacggcgc agattactta gctgctccag
    16381 tgccaggtaa agcgctcgct attttcggcg ataccggccc ctgcgatgcc gcacttgacc
    16441 tggctaaagg tgtcgatgtc atggtgcacg aagcgacgct ggatataacc atggaagcca
    16501 aagccaatag tcgcggccat agctctacac gccaggctgc gacactagcc cgtgaggctg
    16561 gagtcggtaa gctaatcatt acccacgtca gctcgcgcta tgatgacaaa ggttgtcagc
    16621 acctgttacg tgaatgcagg tcaattttcc cggcgactga actagcgaat gatttcaccg
    16681 tgtttaacgt ttaactctgc ttattattaa aaacagggcg aaacttgccc tgttatcgca
    16741 acccgcgcta catatatcca cccacacggg catattgagc gatttttgat atttattcat
    16801 ctccttacgc actatattca gagcaataag ttgcctcttt ttatcttcat tcagcacaac
    16861 ctttgcaact aaaaggagcc catgatggtt acagttgtca gcaattattg tcaattatct
    16921 caaacccaac tcagtcagac atttgcagaa aaatttactg tgaccgagga attactgcag
    16981 tctttaaaaa aaacagcgtt atccggagat gaagaaagta ttgagttact gcataatatt
    17041 gcgttaggtt atgatgaatt tggaaaaaaa gctgaagata ttctttacca tatt
//
LOCUS       DAZGOB010000107        16882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGOB010000107 DAZGOB010000000
VERSION     DAZGOB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.107"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            72..1214
                     /gene="yghO"
                     /locus_tag="ACHLIF_003689"
     CDS             72..1214
                     /gene="yghO"
                     /locus_tag="ACHLIF_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO9607629.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1221..1985)
                     /gene="glcC"
                     /locus_tag="ACHLIF_003690"
     CDS             complement(1221..1985)
                     /gene="glcC"
                     /locus_tag="ACHLIF_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO9607630.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2236..3735
                     /gene="glcD"
                     /locus_tag="ACHLIF_003691"
     CDS             2236..3735
                     /gene="glcD"
                     /locus_tag="ACHLIF_003691"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO9607631.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3735..4787
                     /gene="glcE"
                     /locus_tag="ACHLIF_003692"
     CDS             3735..4787
                     /gene="glcE"
                     /locus_tag="ACHLIF_003692"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO9607632.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4798..6021
                     /gene="glcF"
                     /locus_tag="ACHLIF_003693"
     CDS             4798..6021
                     /gene="glcF"
                     /locus_tag="ACHLIF_003693"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO9607633.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6026..6430
                     /gene="glcG"
                     /locus_tag="ACHLIF_003694"
     CDS             6026..6430
                     /gene="glcG"
                     /locus_tag="ACHLIF_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO9607634.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6452..8623
                     /gene="glcB"
                     /locus_tag="ACHLIF_003695"
     CDS             6452..8623
                     /gene="glcB"
                     /locus_tag="ACHLIF_003695"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO9607635.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPECVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFQVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDAAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8851..9096
                     /gene="traT"
                     /locus_tag="ACHLIF_003696"
                     /pseudo
     CDS             8851..9096
                     /gene="traT"
                     /locus_tag="ACHLIF_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            complement(9129..10871)
                     /gene="cptA"
                     /locus_tag="ACHLIF_003697"
     CDS             complement(9129..10871)
                     /gene="cptA"
                     /locus_tag="ACHLIF_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO9607636.1"
                     /translation="MQKTLTKHEHVFSWKALGWALIYFWYFSTLLQIIICLTGYSGTN
                     GLRDSLLFSSLWLIPVFLFPERIKVTAALIGVVLWGTSLAALCYYLIYGQEFSQSVLF
                     VMFESNTSEASEYFSQYFSLKILFISTIYTAIAILLWTRLSPIYIPSYWRYIIPLFII
                     YGLILHPVLINTVVKNRTIDNTLGRLASRMEPAAPWQFITGYYQYRLQLNSLNRLLEE
                     NNALPALKNFNDISGDTPRTLVLVIGESTQRGRMSLYGYPRKTTPGLDELYKTEQNMF
                     VFNNVVSSRPYTIEVLQQVLTFADEQQPELYLTQPSLMNIMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLAPFSEVLADPAPKKFVIIHLLGT
                     HIKYKYRYPEGQGVFDGDREHAPEGLTDNELELYNDYDNANIFNDHVVATLIKTFKAT
                     APDGFLFYFSDHGEEVFDTPPHKMLGRNEDTPTRPMYNIPFILWASEKWLLNRHHTFS
                     EYINRKYSLADFIHTWSDLAGLRYDNYEPERSILNQHFKEITRWIGNPYKKNALTDYD
                     TLPYAELRVNQASN"
     gene            complement(11290..12054)
                     /locus_tag="ACHLIF_003698"
                     /pseudo
     CDS             complement(11290..12054)
                     /locus_tag="ACHLIF_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            12274..12631
                     /locus_tag="ACHLIF_003699"
                     /pseudo
     CDS             12274..12631
                     /locus_tag="ACHLIF_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(12885..14975)
                     /gene="iha"
                     /locus_tag="ACHLIF_003700"
     CDS             complement(12885..14975)
                     /gene="iha"
                     /locus_tag="ACHLIF_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO9607637.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            14914..15096
                     /locus_tag="ACHLIF_003701"
     CDS             14914..15096
                     /locus_tag="ACHLIF_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607638.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            complement(15357..15710)
                     /locus_tag="ACHLIF_003702"
                     /pseudo
     CDS             complement(15357..15710)
                     /locus_tag="ACHLIF_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16001..>16882)
                     /locus_tag="ACHLIF_003703"
     CDS             complement(16001..>16882)
                     /locus_tag="ACHLIF_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9607639.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRIDGYKGQPVTRLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     KGISPFEYANVNNPLRR"
BASE COUNT         4203 a         4162 c         4406 g         4111 t
ORIGIN      
        1 gattattaag gtgtcgttcg ttgctgttgc aacctgtttt tcaccgttaa ttatttgtta
       61 tccacagaga tatggaatgc gatctgctga tgattaaaat tgaaaaagta attaataaaa
      121 atgaccttaa ggcatttatc gcttttccgt catcacttta tcccgacgat ccaaactgga
      181 tacccccttt attcatagaa cgcaacgagc atttgtctgc gaaaaatccg gggacggacc
      241 atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg cgtattaccg
      301 cgcaaataga taccttgcac cgtgagcgtt acggtgaaga taccggtcat ttcggcatga
      361 ttgacgccat tgatgatccg caggtttttg ccgcgttgtt tggcgcagcg gaagcgtggt
      421 tgaagtcaca aggtgcaagt aagatcagcg gtcctttcag cctgaatatc aatcaggaaa
      481 gcggattact gattgaaggt tttgacacac caccctgtgc gatgatgcca cacggcaaac
      541 cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa aggcattgat ttactggcgt
      601 ggtggatgca gcgaaccgat ctcactttct ctccggcgct aaaaaaactg atggatcagg
      661 tgcgcaaaaa ggtgaccatt cgctgcatca atcgtcagcg gtttgccgaa gaaatgcaga
      721 tcctgcgtga gattttcaac tctggctggc agcacaactg gggatttgtg ccgtttaccg
      781 aacatgaatt cgcgaccatg ggcgatcaac ttaaatatct ggtgccggac gatatgatct
      841 atatcgctga gattgattct gcaccctgcg cgtttattgt cggcttgccg aacatcaacg
      901 aggcgattgc cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc
      961 gtttgaaagt cagcggtgtg cgaactgcgc gagtaccgct gatgggcgtg cgcgacgagt
     1021 atcagttcag ccgcatcggc ccggtgattg cgctgttatt gattgaagcc ttacgcgatc
     1081 cgtttgcccg ccggaagatc gatgcgctgg agatgtcatg gatcctcgaa accaatacag
     1141 gcatgaataa catgctggaa cgcattgggg cggagccgta caagcgttac cgattgtatg
     1201 aaaagcagat ttaataccag ctaactcagg ttcatctcca gcggcaccgc cgagcgaatc
     1261 aaatggtggc cttccagctc gatatcgtgg agattctttt tcacggtccg cacatgatcg
     1321 cgtgctgcgc gctgggcgac gtgcggcagc cgctgcaaca ccgcgttgta gatcctcgca
     1381 tgctggcgat cgatctgctt tttttgctgt ggtcgatggt agagattatt tacgctggca
     1441 aacactgaat taaacatcag atcggtcaat gattgcagcg taaacaccag cacctgattg
     1501 tgagaagcct gacaaatggc gagatggaaa gcgtgatcca actgcgcatg ttcgatcagc
     1561 gaaatctctt tgttgttctc actggcggcg agcatttttt cataacagcg ggttatcaca
     1621 acaaaatcag cctgcgttcc cagcgttgcc gccagccttg ccgattcgcc ctccagtaat
     1681 gcgcgaacgt cgagcagatc gtacagcgtt cgcggctgcg tactgaacag atggatcagc
     1741 ggactggtgt cctgcacccg attaagccgt gcgacacgag aatcgcgacc ctgcgccgtt
     1801 tcaataatcc cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga gcgtgagaag
     1861 ccgagctttt cacacagtcg acgttccgag ggaagtggct gaccgacctt cagtacgccg
     1921 tcgataatta accgttcgat actctctgca accacttcgc aaatagggcg acgttcatct
     1981 ttcattcccg gacctcgtgc acaggtagga ccaatttttc tgttcttaac tcgcaaaaca
     2041 cgcacatcac gtaagtgatt atgttacatc aatttaacat tgagttaacc aaggcaaggt
     2101 cacagagctg gaaaaaaaat ggtctgaccg gtagctaaag agatagacga aaacgaaaag
     2161 cccgcttaat aactgttcac agaagcagcg cgcaaaaatc agctgccaca caacacaaca
     2221 aagcgaagcc tactcatgag catcttgtac gaagagcgtc ttgatggcgc tttacccgat
     2281 gtcgaccgca catcggtact gatggcactg cgtgagcatg tccctggact tgagatcctg
     2341 cataccgatg aggagatcat tccttacgag tgtgacgggt tgagcgcgta tcgcacgcgt
     2401 ccattactgg ttgttctgcc taagcaaatg gaacaggtga cagcgattct tgctgtctgc
     2461 catcgcctgc gtgtaccggt ggtgacccgt ggtgcaggca ccgggctttc tggtggcgcg
     2521 ctgccgctgg aaaaaggtgt gttgttggtg atggcgcgct ttaaagagat cctcgacatt
     2581 aaccccgttg gtcgccgcgc gcgcgtgcag ccaggcgtgc gtaacctggc gatctcccag
     2641 gccgttgcac cgcataatct ctactacgca ccggaccctt cctcacaaat cgcctgttcc
     2701 attggcggca acgtggcaga aaatgccggc ggcgtccact gcctgaaata tggtctgacc
     2761 gtacataacc tgctgaaaat tgaagtgcaa acgctggacg gcgaggcgct gacgctggga
     2821 tcggacgcgc tggattcacc tggttttgac ctgctggcgc tgttcaccgg atcggaaggt
     2881 atgctcggcg tgaccaccga agtgacggta aaactgctgc cgaagccgcc cgtggcgcgg
     2941 gttctgttag ccagctttga ctcggtagaa aaagccggac ttgcagttgg tgacatcatc
     3001 gccaatggca ttatccccgg cgggctggag atgatggata acctgtcgat ccgcgcggcg
     3061 gaagatttta ttcatgccgg ttatcccgtc gacgccgaag cgattttgtt atgcgagctg
     3121 gacggcgtgg agtctgacgt acaggaagac tgcgagcggg ttaacgacat cttgttgaaa
     3181 gcgggcgcga ctgacgtccg tctggcacag gacgaagcag agcgcgtacg tttctgggcc
     3241 ggtcgcaaaa atgcgttccc ggcggtagga cgtatctccc cggattacta ctgcatggat
     3301 ggcaccatcc cgcgtcgcgc cctgcctggc gtactggaag gcattgcccg tttatcgcag
     3361 caatatgatt tacgtgttgc caacgtcttt catgccggag atggcaacat gcacccgtta
     3421 atccttttcg atgccaacga acccggtgaa tttgcccgcg cggaagagct gggtgggaag
     3481 atcctcgaac tctgcgttga agttggcggc agcatcagtg gggaacatgg catcgggcga
     3541 gaaaaaatca atcaaatgtg cgcccagttc aacagcgatg aaatcacgac cttccatgcg
     3601 gtcaaggcgg cgtttgaccc cgatggtttg ctgaaccctg ggaaaaacat tcccacgcta
     3661 caccgctgtg ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc tttccctgaa
     3721 ctggagcgtt tctgatgcta cgcgagtgtg attacagcca ggcgctgctg gagcaggtga
     3781 atcaggcaat tagcgataaa acgccgctgg tgattcaggg cagcaatagc aaagcctttt
     3841 taggtcgccc tgtcaccggg caaacgctgg atgtgcgttg tcatcgcggc attgttaatt
     3901 acgacccgac cgagctggtg ataaccgcgc gtgccggaac gccgctggtg gcgattgaag
     3961 cggcgctgga gagcgccggg caaatgctcc cctgcgagcc gccgcattat ggtgaagaag
     4021 ccacctgggg cgggatggtc gcctgcgggc tggcggggcc gcgtcgcccg tggagcggtt
     4081 cggtccgcga ttttgtcctc ggcacgcgca tcattaccgg cgctggaaaa catctgcgtt
     4141 ttggtggcga agtgatgaaa aacgttgccg gatacgatct ctcacggtta atggccggaa
     4201 gctacggttg tcttggcgtg ctcactgaaa tctcgatgaa agtgttaccg cgaccgcgcg
     4261 cctccctgag cctgcgtcgg gaaatcagcc tgcaagaagc cattaacgaa atcgcccagt
     4321 ggcaactcca gccgctaccc attagtggct tatgttactt cgacaatgcg ttgtggatcc
     4381 gccttgaggg cggcgaagga tcggtaaaag cggcgcgtga actgctgggg ggcgaagagg
     4441 ttgcgggtca gttctggcag caattgcgtg aacaacaact gccgttcttc tcgttaccag
     4501 gtaccttatg gcgcatttca ttacccagcg atgcgccgat gatggattta cccggcgagc
     4561 aactgatcga ctggggcgga gcgttacgct ggctgaaatc gacagccgag gacaatcaaa
     4621 tccatcgcat cgcccgcaac gctggcggtc atgcgacccg ctttagtgcc ggagatggtg
     4681 gctttgcccc gctatcggct cctttattcc gctatcacca gcagcttaaa cagcagctcg
     4741 acccttgcgg cgtgtttaac cccggtcgca tgtacgcgga actttgagga gcaggctatg
     4801 caaacccaat taactgaaga gatgcggcag aacgcgcgcg cgctggaagc cgacagcatc
     4861 ctgcgcgcct gtgttcactg cggattttgt accgcaacct gcccaaccta tcagcttctg
     4921 ggcgatgaac tggacgggcc gcgcgggcgc atctatctga ttaaacaggt gctggaaggc
     4981 aacgaagtca cgcttaaaac acaggagcat ctcgatcgct gcctcacttg ccgcaattgt
     5041 gaaaccacct gtccttctgg tgtgcgctat cacaatttgc tggatatcgg gcgtgatatt
     5101 gtcgagcaga aagtgaaacg cccactgccg gagcgaatgc tgcgcgaagg attgcgccag
     5161 gtagtgccgc gtccggcggt cttccgtgcg ctgacgcagg tagggctggt gctgcgaccg
     5221 tttttaccgg aacaggtcag agcaaaactg cctgctgaaa cggtgaaagc taaaccgcgt
     5281 ccgccgctgc gccataagcg tcgggtttta atgctggaag gctgcgccca gcctacgctt
     5341 tcgcccaaca ccaacgcggc aactgcgcga gtgctggatc gtctggggat cagcgtcatg
     5401 tcagctaacg aagcaggctg ttgtggcgcg gtggactatc atcttaatgc gcaggagaaa
     5461 gggctggcac gggcgcgcaa taatattgat gcctggtggc ccgcgattga agcaggtgcc
     5521 gaggcaattt tgcaaaccgc cagcggttgc ggcgcgtttg tcaaagagta cgggcagatg
     5581 ctgaaaaacg atgcgttata tgccgataaa gcgcgtcaag tcagtgaact ggcggtcgat
     5641 ttagtcgagc ttctgcgcga ggaaccgctg gaaaaactgg cagttcgcgg cgataaaaaa
     5701 ctggccttcc actgcccgtg taccctacaa catgcgcaaa agctgaacgg cgaagtggaa
     5761 aaagtgttgc ttcgtcttgg atttacctta acggacgttc ccgacagcca tctgtgctgc
     5821 ggttcagcgg gaacatatgc gttaacgcat cccgatctgg cacgccagct gcgggataac
     5881 aaaatgaatg cgctggaaag cggcaaaccg gaaatgatcg tcaccgccaa cattggttgc
     5941 cagacgcatc tggcgagcgc cggtcgtacc tcagtgcgtc actggattga aattgtagaa
     6001 caagcccttg aaaaggaata aaaaaatgaa aactaaagtc attcttagcc agcaaatggc
     6061 gagtgcaatt attgccgcag gtcaggaaga ggcgcagaaa aataactggt cggtttccat
     6121 tgctgttgcc gatgacggcg gtcatctgct ggcgttaagt cgcatggacg attgcgcgcc
     6181 gattgcggct tacatctccc aggagaaagc gcgtaccgcc gcgctggggc gtcgtgaaac
     6241 taagggctat gaagagatgg tgaacaacgg acgtaccgcg ttcgtgactg cgccgttact
     6301 gacgtcgctg gaaggcggcg taccggttgt tgtggatggg caaattattg gtgccgtggg
     6361 cgtttctggt ttaaccggag cacaggatgc gcaggtcgcg aaagcggcag cagcggtgtt
     6421 ggcgaaataa gcgaaaacga ggagagaaac aatgagtcaa accataaccc agggccgttt
     6481 acgcattgac gccaatttta aacgttttgt ggatgaagaa gttttaccgg gaacagggct
     6541 ggacgctgcg gcgttctggc gcaattttga tgagatcgtt cacgatctgg caccagaaaa
     6601 tcgtcagttg ctggcagaac gcgatcgcat tcaggcagcg cttgatgagt ggcatcgcag
     6661 caatccgggg ccggtaaaag ataaagcggc ctataaatct ttcctgcgtg aactgggcta
     6721 cctggtgccg caaccggagt gcgtgacggt ggaaaccacg ggcattgaca gcgaaatcac
     6781 cagtcaggca gggccacagc tggtggttcc ggcaatgaac gcccgctacg cgctgaacgc
     6841 ggcgaacgct cgctggggct cactgtacga tgcgttatac ggcagcgaca tcatcccgca
     6901 ggaaggggcg atggtcagcg gctacgatcc gcagcgcggt gagcaggtta tcgcctgggt
     6961 tcggcgtttc ctcgatgaat ctctaccgct ggaaaacggc agctatcagg atgtggtggc
     7021 gtttcaggtg gtcgataaac aattacgcat ccagttgaaa aatggtaaag aaaccacgtt
     7081 acgtaccccg gcgcagtttg tcggttaccg tggcgatgcc gctgcgccga cctgcatttt
     7141 gctgaaaaat aacggcctgc atattgaact gcaaattgat gccaacggac ggattgggaa
     7201 agacgatgcg gcgcacatca acgatgttat cgtcgaagct gcaatcagta ccattctcga
     7261 ctgcgaagat tcggtcgcgg cggttgatgc ggaagataaa atcctgctgt accgcaatct
     7321 gctgggcctg atgcagggga ctctccaaga gaaaatggag aaaaacggtc gacaaatcgt
     7381 gcgtaaactg aatgacgatc gtcagtacac cgccgccgat ggctctgaaa tttctctgca
     7441 cggacgctcg ctgctgttta tccgcaacgt gggtcatttg atgaccattc ctgtgatttg
     7501 ggacagcgaa ggcaatgaaa tcccggaagg cattcttgat ggcgtcatga ctggcgcgat
     7561 tgccctctat gatttaaaag tgcagaaaaa ctcgcgcact ggcagcgtct atattgtgaa
     7621 accgaaaatg cacggtccgc aggaagtggc gttcgccaac aaactgttta cccgcattga
     7681 gacaatgctc ggtatggcac cgaataccct gaaaatgggc attatggatg aagaacgtcg
     7741 gacctcgctg aacttgcgta gctgtatcgc tcaggcacgc aaccgcgtgg cgttcatcaa
     7801 taccggtttc ctcgaccgta ccggcgatga aatgcattcg gtgatggaag ctggcccgat
     7861 gctgcgtaaa aatcagatga aatcgacgcc gtggatcaaa gcctacgagc gtaataacgt
     7921 gctttccggc ctgttctgtg ggctgcgcgg taaagcgcaa atcggtaaag gcatgtgggc
     7981 aatgccggac ctgatggcag acatgtacag ccagaagggc gaccaactgc gtgccggggc
     8041 aaacacagcc tgggttccgt cgccaactgc cgctacgctc catgcgatgc actaccacca
     8101 aaccaacgta cagagcgtgc aagccaacat tgcccagact gagttcaatg ctgaatttga
     8161 accgctgctg gacgatctgc tgactattcc ggttgctgaa aacgctaact ggtcggcgca
     8221 agagatccaa caagagctgg ataacaacgt gcaggggatt ctggggtacg tggtgcgctg
     8281 ggtggagcag gggattggtt gttcaaaagt gccggatatt cacaatgtgg cgctgatgga
     8341 agaccgcgca acgctgcgta tctccagcca gcatatcgcc aactggttac gtcacggtat
     8401 tctgaccaaa gaacaggtgc aggcgtcgct ggagaatatg gcgaaagtgg ttgatcagca
     8461 aaacgctgac gatccggctt atcgtccgat ggcggggaat ttcgctaact cgtgtgcttt
     8521 taaagctgcc agcgatttaa tcttcctcgg cgtgaaacag ccaaacggct ataccgaacc
     8581 gttattacac gcctggcgtt tacgcgaaaa agaaagtcat taatagcgtc aaaaaaagcc
     8641 ccggcaagtt ttattgtcgg ggctatgata gtaactgcgg tagtacattt atcccggaaa
     8701 taaccggagc gattatcagg gtttaacaat attaaatacc cgtagcgcat tactagcggg
     8761 cctataacaa caatatggac atcttaacga tactctgcgc ctgcgtgtgc agaacagaaa
     8821 cccttacccg atatacttgt tatgccagag gtagcaattg cggcaaccga tctgactggc
     8881 atggatgcag atgcaatggt ggaagatata aattacacga taattacaga tgttcagatt
     8941 accgaacgaa ctaaaaccta tgtcagagca gataatgcta ccacttttcg gcagggaaca
     9001 tcaggcaccg aaattcaggc tagcacagaa accagcaacc aggttaatct gaaatttgaa
     9061 ggggctaaac ctcttctgga aatacaactg aaataaatat cctccggcta tgccggagga
     9121 tatttatttt aatttgaagc ctgatttaca cgcagctctg cgtagggcag ggtatcatag
     9181 tcagttaacg catttttctt ataggggttt cctatccagc gagtgatttc tttgaaatgc
     9241 tggtttaata tggaacgctc tggctcatag ttatcataac gtaaaccagc caaatccgac
     9301 caggtatgga taaaatcagc cagactgtat ttacggttaa tgtactcaga aaatgtatga
     9361 tgtcgattaa gtagccattt ttcagaggcc cagagaataa atggaatatt gtacataggt
     9421 cgggttgggg tgtcttcgtt acgtcccagc attttatgtg gtggcgtatc gaatacttcc
     9481 tctccatggt cagaaaaata gaaaagaaaa ccatcagggg ctgttgcttt gaaagttttg
     9541 atcaacgttg caacaacatg gtcattaaat atattagcat tatcgtaatc gttatataat
     9601 tccagttcat tatcagtaag tccttctggt gcatgttccc tgtccccatc gaatactccc
     9661 tgaccttcag gataacggta tttatatttg atgtgtgttc ccaacaagtg aataataaca
     9721 aattttttgg gagctggatc tgccagtact tcactaaacg gagccagtac gttagtatcg
     9781 tactcacggg cactttgcgt tctctgctga ttcatgtagt actgtttatc tgtttgccga
     9841 gagaagactg tcagcattgt gttgcgtgca gtcatcgttt gttgattggt gatccagaat
     9901 gttttgtaac cggcctgttt cattatattc atcagggaag gttgtgtcag gtagagttcg
     9961 ggctgctgtt catcagcgaa agtcaatacc tgctgtagta cttcgatggt atatggtctt
    10021 gaggagacaa cattattaaa cacaaacata ttttgttccg ttttgtataa ttcatctagt
    10081 ccgggggttg tttttctggg atatccatac agactcattc tcccacgctg ggttgattct
    10141 cctatcacca gaaccagtgt tcgtggggta tctccggaaa tatcgttaaa gtttttcaga
    10201 gcggggagcg cattattttc ctccagcaaa cggttcagag aattaagctg gagacggtac
    10261 tgataatatc cggtaataaa ttgccagggg gcagcgggct ccatacggga tgccaggcgt
    10321 cccagagtat tgtcaatagt cctgttttta acaacagtat ttattaatac agggtgcaga
    10381 attaaaccgt agataataaa taaaggtata atgtagcgcc agtatgatgg tatatatatc
    10441 gggctaagac gagtccatag cagaatagca atagctgtat atattgtcga tataaatagt
    10501 atttttaaac taaaatactg actaaaatat tcgctggctt cacttgtatt actttcaaac
    10561 atgacgaaca gtacactctg agagaattcc tgcccgtaga ttagataata gcaaagggca
    10621 gcaagagatg tgccccataa aaccacgccg attaatgctg ctgtaacctt tattctctct
    10681 gggaaaagaa aaacagggat taaccagaga gagctgaaaa gtagcgagtc acgaagtcca
    10741 ttggtgccac tatatccagt cagacaaatg ataatctgaa gtaaagtgga aaaataccag
    10801 aaataaatga gagcccatcc cagggctttc cagctaaaaa catgttcatg ttttgttaat
    10861 gtcttctgca tattaataaa cctgcacggg aatttactat tgtaattttt atatgagatg
    10921 tattaaaaca tttcatacag attttttgat aagcacatag tttcaatatt cactataatc
    10981 gttttctgct gtgttgtgtt tgatcctttt tcaaagaggc gctaatcttc cacttgccaa
    11041 ggttagatgg tcagggtccg ggttaaacaa aatgtttgac agggagcctt tccatatgct
    11101 gtctttccag cgtggcggtc cgtaatgtac taattttaag agaggtccgc aatcttccag
    11161 tggatggttc atctgcctct ctattaccgt taccatcgtc ttgcgtcctg tgctatcgcc
    11221 tgtaacgaca tcagcgtcac tggtattaac aaacttatag ttgaagccag tcacttcgtc
    11281 tcttggctaa cgcagcgtga tgagctggag tgccaggcgt gggagaacaa cgacagcagc
    11341 attcgtaccg gggacgatga cggtttgctg tgtcagtgcc tgggggatat ctgcagcaag
    11401 gcgttttccc agacggggcg cgagtttgca gagctgggtt gccagttttt tctcacccag
    11461 taaagcgagt ttttcgggtg agggatatcg ctggagaaga tcaagtactg caggatgatc
    11521 cagtcgcggg ccgagaacgc gctccagagc cggatgtatc tgggtcaaca agccgcggat
    11581 gcggttgctg gcctgtgttg tctgtgcggc aagatcatca tcgaagccgc aaagcatgga
    11641 gagttcggcg atttgttcgt cggccagttt tagagagcga agagcatgag gcagagtacg
    11701 tgcagcttcg gcaatgatgg ccgcatcacg ggcatcggtt ttggcttcac cggagtgcaa
    11761 atcggcgatg cgtcgcattg ccagtccagg aaggtatccg acaaggacgc cttcagagcg
    11821 ggcaacggcg acgggtaacg ccctgatggt agcaggctgg tcaacgacca gcagtatctg
    11881 tccatgctgt ttcaggtcag atatcagcgc tctgagtttg ttttcgtcgt tgggtaatac
    11941 tctgtcgaac aaacgtttac ctgaacgatt aacggcaacg gcgtaatggg tgtctttacc
    12001 tacatcaacg ccaataaaga ccaggattga ttcgtaatcg ctggattcgg gcattgtgtc
    12061 tccccgttta tacgggttaa ccagataacc ttggggagca ggtaccggca tccacgttac
    12121 agactgtccc gacatgatgt ctgaccaggc ccctatcagc ggttaccagc gccccaccag
    12181 acccggtgac atccccccga atcatggacg actgggggaa ttaatcatgc cggggctggc
    12241 taacacccca ttacaagggt acgaataaag taacggggag ttacaggcac tgaaactatc
    12301 atcagagata ctgttgccaa atggcgtaaa ggttggatcc cgccgtttgc tattgccgcc
    12361 cgacttcctt cactgtccct gggaagctgc ggggtggtgc cctggaaaat aatcaggggg
    12421 aagaaaatta tgcgtcccga tttgtgaaac agaaccggaa cagaaaatag cgcagcaact
    12481 ggcactcgag ttctaccgta ttctgccccc caaataaatc acagctaaac agttggttca
    12541 ctcaagtcca cgaaagcggt tcactagaat ttcagcgcgt agccacgggg atggagccga
    12601 tactgcagct atatgtgaga caattgtcag cgccagtgat ataagacggt aatggagatg
    12661 cggacgcggc ccttaattca gagctagccg agtataaatt tctggacaag tatgtaaatg
    12721 atgagttaaa aaataatccg tgcatttccg gtgatgatat tagttgttgg taacgaaatc
    12781 tcattagcgg accggaaatg cacttaacct aatacgttgc cactgttccg ccaggagttg
    12841 cagtggcaac gtattctacc gtcagtgata gcgttttgtt attatcagaa ctgatagttc
    12901 agcgacatcc agtaatttcg ctcaggtatc acatatcctg ttgttgatga tcccgtctgg
    12961 aagtaatcac cggcatacag cgtactctta ccggcactgt acaggctcac gtcactgtaa
    13021 tccttgttga gcaggttatt caccgcagca ttcagcgtca gggcatccgt catcttccac
    13081 gacagacctg catccaccac cgtccaggct ttcaggtatt ctcctttctc atcatacact
    13141 ttcttctgta cagcgctcag tgacgaataa ttctgggtga aacgtggtgt tttcccgcga
    13201 taacgggcac ccagccatga tgccacctct tcggtgatct gccagttcag tttcgcattc
    13261 accatgtgtt caggggtata actcagcggc gcacctttgt tatcaccatc acgttgttca
    13321 cttcgggtcc aggtgtaatt cagtgacagc gtgacatcct ctgaccacag cggcaatgtg
    13381 ccggcaaatt ccacaccgtg caaccgggcc tttccgctgt ttacatagct attggtgtta
    13441 tcatttatgg aataagagac aatcttgttg gagaagtcag tcataaaacc tgtgacattg
    13501 gcattcagac cggcggggtt atcgtaatac accccagcct cataactgac gctctcttcc
    13561 ggcttcaggt cggggttacc aagtagattt gtttttccct gcccggacac accactaatc
    13621 cctttatgta gctgccccat tctgggtgcc ttatatcccg tggtcacacc gcctttcagc
    13681 gtccaggcat ctgccacatc ccagaccaga tatgcacgcg gactgaagtg tcccccgaat
    13741 tgctcatgat gttcatagcg gctgcccgca gtcagcgcaa gtgcatccgt gagatgccac
    13801 tcatcctcag caaataccga ccagcttttc tgccggaaag tttcacctgt gctggcaagg
    13861 acaactccgt ctttcatgga cgagctctga aactcgcccc ccaccgtaac cagatgagat
    13921 tctcccagag gggtaagcag taatgaattc aggataaggt tcgattcctt aagctcccgc
    13981 ggctgaccgg caagccccca tttgtcgcgc ttcagtacac tgcgtacaag ctcacgacct
    14041 ttattttctg tctcgttcca gttcagatac gatttccatg ttccgaaggt gaaagtatga
    14101 tcatagccag ctgaaatttt gtttcgctca tagcgcaggg tccggtcata tccccccgtc
    14161 agactcccca gttgcccatc ccggttatca taacgctgcc gggtggtatc catatcaaac
    14221 cagagcacat cctgctccga cgccttccag tcaagacgtg caccaagatt ataattctgt
    14281 gactccgtgg gataaggaat acgcgtggct gctgtatcgc tcagtgatgt gaccgatgaa
    14341 ccctgacgct gttgtgtgct accgcgtacc tgcaggctga cagaatcatc cacaagggga
    14401 ccactgctcc agaaattaaa ctggctgctg ttaccccatt tgttgctttc ctgcagattc
    14461 agccctgcat tgacggaaga gagccatttg tctgcattct ttctggtaat gatattcacc
    14521 acaccgccca tcgcatcaga gccatacagt gtggacatcg gccccctgat aacctcaata
    14581 cgctcaatgg cggccagagg gggcatgaac ccggtattca tggcagaaaa accgttggga
    14641 gtcacgtcac tgcttccgcc ctgacgaaca ccatcaatca gtatcagcgt gtaactggct
    14701 ggcattcctc ggatgctgat ttccagccct ccggttttac ccgtaccact ttcaacatcc
    14761 acaccctcta ctgatctcag agcctccgcc agatcgtggt actggctgga ctgcaattcc
    14821 tcctggctaa tcacagaaac actggcggct gcgttagtca gctttttctc atagccggat
    14881 gccgagacaa tcatcacatc ctctgcagca gctatgctgc tggctgaaaa tccgagacag
    14941 ggaatgacta cggaagccag agtggttatt cgcatattac taatctccga tcatgttaac
    15001 cggcaagaca atacacaaat acaaacaata atgagaataa tttatatttg ccttttacaa
    15061 aaaataaaga ttattgacaa aaccaaatat aaataacgac tgcctcggca tgccagttaa
    15121 cagctatccg tatggcgtgt tcggttttgc gaataaaaaa tggttactgt agaagcggat
    15181 ttccaccaca tgtcaaccat gaatggtcag tgatgcgaga cgactgggat gatttggtcg
    15241 atgacttctt ccggcgaata ctgatagaaa cattgaaata ttgtaagaga aagcgttcaa
    15301 aattctgtgg cacgtgaaaa attcgtaagc gccccgagaa gtacgtagcg taaggattat
    15361 tttacagttg agcagttcca gggcagcagt tcatgcacac ggttcgacgg ccagtcattg
    15421 agcttcacga tcacttcgcg caaccagtcc tccggctcca cttcgttcag tttgcaggtg
    15481 accagcagac tgtagatgat cgccgcgctt tctcccccct tgtctgagcc gaagaagaga
    15541 taattttttc ttccaaccgc caccgatcgt aacgcgtttt caccgatgtt gttgtctatt
    15601 tccacccagc cgtcacgaca gaactcgttc agcgcattcc agtgattcag gatatagtcg
    15661 aacgccttta ccatttttgc atgtttatac agagttttat tctgtaattg atttatatta
    15721 ttgtcgaata gtttatattg gaagggaaaa cactttttcc gttcaggaag ctatatcgat
    15781 tagtcacatg ccccaaatgg aggatcaatc aaattctgcc taaaaccaca ctttaagctc
    15841 ctgctgagat actccttctc tgtcaactta tacgccgaat gctaagtggc tatttagagg
    15901 taacgggtga ttgcggtgct atcagccgga ataaaaaaga aatttattgg agcaggttgc
    15961 tcttccccat gggaagagca tgtgtgtgaa tagccttctg tcaccttctc agcggattgt
    16021 taacatttgc gtattcaaac ggactgatcc ccttctcccg gttcgcatca agaaaatcgg
    16081 cccaccactg tagcatcagt cgacgttctt ccagatgttc tgctttatgg atatacgcag
    16141 cacgtacacc attacgctcc tgatggctca tctgacgttc cacagcatcg cgggaccaca
    16201 aacctgattc aatcaatgca ctgcacgcca tcgcccggaa tccatggcca cagacatcct
    16261 gggttgtgtc ataccccatc accctcaggg ctttatttac agtattttca ctgattggtt
    16321 tacgcgggtc atgtgctccc gtaaagatca gaccattttc accagcccat gtctgtagtt
    16381 ctgtcagtat ggctacagcc tgtttgctga gtggcacaag atgtggtgtt cgcatcttgg
    16441 agccccggtg tgagaatttt acgcctggca gaggttcgcg ttcggctgga atagtccaca
    16501 tggcattgtc aatatcaatt tcagaccagc gggcatatct gagttcactg gaacgaataa
    16561 aaaccagtaa attcagcata acggcaagcc gggtgacagg ctggccttta taaccgtcaa
    16621 tacgcgacag aagttcaggc aggcgtgaaa gattcagagc ggggcgatgc tggcgtttta
    16681 ccgtggtcaa tgcaccagcc atatccagag ccgggttata tctgatgatg ccagactgta
    16741 cggcgtaacg cattacagca gatattcttt gctgcagacg actggcgatt tcataaattt
    16801 gttttgcttc tgcagcacga acagggataa gcaggtccgg ggtattgagt gttgtgatat
    16861 cccggttgcc gataaagggg aa
//
LOCUS       DAZGOB010000108        16645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGOB010000108 DAZGOB010000000
VERSION     DAZGOB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.108"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(37..189)
                     /locus_tag="ACHLIF_003704"
     CDS             complement(37..189)
                     /locus_tag="ACHLIF_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9607640.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHLIF_003705"
     CDS             complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHLIF_003705"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO9607641.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHLIF_003706"
     CDS             complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHLIF_003706"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO9607642.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHLIF_003707"
     CDS             complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHLIF_003707"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO9607643.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5092..5454
                     /gene="queD"
                     /locus_tag="ACHLIF_003708"
     CDS             5092..5454
                     /gene="queD"
                     /locus_tag="ACHLIF_003708"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO9607644.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5532..6803
                     /gene="ygcN"
                     /locus_tag="ACHLIF_003709"
     CDS             5532..6803
                     /gene="ygcN"
                     /locus_tag="ACHLIF_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9607645.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6794..7054
                     /gene="ygcO"
                     /locus_tag="ACHLIF_003710"
     CDS             6794..7054
                     /gene="ygcO"
                     /locus_tag="ACHLIF_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO9607646.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCCLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7071..7646
                     /gene="ygcP"
                     /locus_tag="ACHLIF_003711"
     CDS             7071..7646
                     /gene="ygcP"
                     /locus_tag="ACHLIF_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO9607647.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACHLIF_003712"
     CDS             complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACHLIF_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO9607648.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACHLIF_003713"
     CDS             complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACHLIF_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO9607649.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRIEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACHLIF_003714"
     CDS             complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACHLIF_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9607650.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10839..12293)
                     /gene="ygcU"
                     /locus_tag="ACHLIF_003715"
     CDS             complement(10839..12293)
                     /gene="ygcU"
                     /locus_tag="ACHLIF_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO9607651.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPQEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGTDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12363..13148)
                     /gene="ygcW"
                     /locus_tag="ACHLIF_003716"
                     /pseudo
     CDS             complement(12363..13148)
                     /gene="ygcW"
                     /locus_tag="ACHLIF_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13466..14743
                     /gene="yqcE"
                     /locus_tag="ACHLIF_003717"
     CDS             13466..14743
                     /gene="yqcE"
                     /locus_tag="ACHLIF_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO9607652.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEVRTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14770..16248
                     /gene="ygcE"
                     /locus_tag="ACHLIF_003718"
     CDS             14770..16248
                     /gene="ygcE"
                     /locus_tag="ACHLIF_003718"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO9607653.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGSVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3970 a         4366 c         4184 g         4125 t
ORIGIN      
        1 caattcagta cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag
       61 aactgcctta aactccatac cacgttctct gatactcaac tcacacaacg agtcacctac
      121 catcagcgtg aatcccagta ctgtacaaca cagtacgatg attgccacaa gggcatattt
      181 tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga
      241 acgtaagcct caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg
      301 aatgcttaac gccagacagc ctcaagcacc cgacgccatt ctatacctga taattcttcc
      361 agcggcgttc acgtttaaaa tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt
      421 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgcaatc cacattccct
      481 ttttaagcca aagaaacgtg tttcttcttc cgacatgcca ggttcccatt tacgggttgt
      541 atgggtatcg cccaccgata aatatccttc gtcccataat gggtgatatt tcaggccatg
      601 tttttgcaga tactgatata tcgttcggtt atcccagtcg ataatcggca gcactttaaa
      661 tacgccacgc tgaattgcca gcaccggtaa attggcgcgg ctgccggatt gttcgcggcg
      721 caggccagca aaccacgttt gcgcattcag ttctttcagc gcccggttca tcggttcgac
      781 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgttcccaca gtttgccgta
      841 gcgtgcttcc tgccaggcgg cgctttcggt agcgcggtac actttcaggt tgagcttgag
      901 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacagatagc cggtgtcggt
      961 gaggatcacc ggaatatccg ggtgaatctg attcaccaga tgcaggctca ccgccgcctg
     1021 aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggcaac
     1081 gcggccttca gcgtccagtt tttccagttc ggcgttagtt tctgccagcg ccagaatgcg
     1141 atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact
     1201 gtttgccttg cctgatgcga cgctcacgcg tcttatcagg cctacaagac cgggctgatg
     1261 gttaatccca caaatcgcgc gccggatcga gcaccgggcg aatgatgccc gcacgcaccg
     1321 taaagtcgcc gaagccttca cccgcttcgc gctctttcgc ccagcgccct atcagttcat
     1381 caagcgacgc caggatttcc ggctcggtga tgttttcttt atacatccgt gggatacgtg
     1441 tcccaatgcg gttgccgcca agatgcaggt tgtagcgacc cggcgcttta cccaccaggc
     1501 ccacttccgc cagcatcgcg cgaccacaac cgttcgggca gcctgttaca cgcatcacga
     1561 tatgctcatc gctgacacca tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg
     1621 gcaggaaacg ctctgcttcc gccatcgcca gcgggcaagt cgggaacgag acgcaggcca
     1681 tcgagttttc acgctgcggc gtgacggcat tcattaaccc gctctctttg gcgatcttct
     1741 cgatcttcgc tttctcgctt tccggtacac cggcgatgat cagattctgg ttcgccgtaa
     1801 tgcggaaatc gcctttgtgg atcttcgcga tctccagcag gccggttttc agcggacgcc
     1861 ccggataatc aaggatgcga ccattttcga taaacagcgt caggtgccag ttatcatcaa
     1921 tgcccttaac ccagccaata cgatcgcctc gtccggtgaa ctcatatgga cggatcggtt
     1981 caaatttgat ccccgcgcga cgctccactt ccgctttaaa cgtctcaacc cccacgcgct
     2041 ccagcgtgta tttggttttg gcatttttac gatcggttcg gttaccccag tcacgctgag
     2101 ttgtcacgac ggcttcggcc accgccagcg tatgctccag cggcagatag ccaaactcgc
     2161 tcgccgtgcg ggcgtaggtt ttcttgttgc cgtgttcgat agaaagccca ccgcccacca
     2221 gcaggttgaa gcctaccagc ttgccgtttt cggcgattgc cacgaagttc atgtcgttgg
     2281 catgcagatc gatatcgttc tgcggcggga tcactaccgt ggttttgaac ttacgcggca
     2341 ggtaggtctg accaaggatc ggttcttcat cagtagtggc gactttttcg tggtcgagcc
     2401 agatctccgc atacgcgcgg gtacgaggca acagatgctc agaaatcttc ttcgcccact
     2461 cgtacgcttc cgcgtgcagc tgcgactcgt aagggttcga ggtgcagagt acgttacggt
     2521 tcatgtcgtt agctgtcgcc agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg
     2581 gtttgacgtt ctttttcaga atgccgtgga actgaaacgt ctggcggttg gtcaggcgaa
     2641 tgctgccata gatggtgttt tcaccggcaa atttgtcgat cgcctgccac tgtttagtgg
     2701 taatcacccc acccggcaga cgacagcgaa gcagcatcgc gtggcgcggc tccagcttct
     2761 gttcagcacg ttcggcgcgg atgtcgcggt catcctgctg atacatgccg tggaagcgga
     2821 tcagcaggaa gttgtcgccc ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa
     2881 tggtgccgcg caggtagttg ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt
     2941 cgaccactaa aggccctgga tgtttttcgc tcattagtag acatctcgct gataacggcg
     3001 ctctacgcgc agctcactta aaaattcatc cgccgcttcg gtgtccatgc caccaaattc
     3061 ggcaatcact tccagaagtg cctgctcaac gtctttcgcc atgcgattag cgtcgccgca
     3121 gacataaatg tgggcaccat cattgatcca gcgccacagc tccgcgccct gttcgcgcag
     3181 tttgtcttgt acgtaaactt tttctttttg gtcgcgcgac caggcaagat cgatacgtgt
     3241 cagcacgcca tctttgacgt agcgctgcca ctccacctga tacaggaagt cttccgtaaa
     3301 gtgcggatta ccaaagaaca gccagttttt acctggcgct tcgtcggcgg cgcgttgctg
     3361 cataaaggcg cggaacggcg caataccggt gcctgggcca atcataatca ccggggtttc
     3421 cgggttagcg ggcaggcgaa agttatcgtt atgttcgata aatacgcgga cttcgccctc
     3481 ttcttccacg cgatccgcga ggaagctgga ggcaccaccg gcacgggctc ggccttccac
     3541 gtcgtaacgc accacaccaa cggtgacgtg tacttcgttc tcgacttccg cctgcgagga
     3601 ggcgatggaa tacaggcgcg gggtcagcgg gcgcagcaga ttaatcagcg cttcggcatc
     3661 cagttgcgcc ggagagaaac gcaccatgtc gacaatcggc gtcgtcgcgg cgtaatgctg
     3721 taacttcgct ttatcgccca ccagcggcag cagtgtttcg ctgcgggtaa gcgtggcgta
     3781 attctcaaca atgttggcgg tgttgacggt cagttcgaag tgccactgta gcgcttcgtt
     3841 cagaggcaac gttttgccct cgacggtgac aggttcatcg cctttcagcc acagcagttc
     3901 gacaagttct ttcaccagtg ccggatcgtt ctgataccag acgcccagcg cgtcacccgg
     3961 ctggtaacgc aggcccgagt cacctaagtc aatttcgata tggcgaacgt ctttttcaga
     4021 gttacgcccg gtaattttct ggttaacaga gaggctagcc accagcggcg cgtctttgct
     4081 gtacgggctg gtgtggattt catttaccgc gccagtagcg acggattgcg aaggtgccgc
     4141 gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc
     4201 ctggtattca acatcggcat cgacacggtc gagcaggcgt tcaccaccca gttccgccag
     4261 cttgctgtcg aaatctttcc cggactggca gaaaaattca taagagctat cgccgaggct
     4321 aaacacggca aacgcggtgt tttccagctt tggcgctttt ttggagaaca ggaacttatg
     4381 cagcgcgacg gcttcttccg gcggttcccc ttccccttgc gttgacgtca ctacgatgag
     4441 cagtttttcg ctggcgattt gtttgaattt atagtcgccc gcgttcacca gcttaacgtt
     4501 cagttttgct gctaataaat catcgcgtaa tgcttcagca acccggcgcg cattgccggt
     4561 ttgcgaggcg gagataatag ttatacccgg catttctgcg gctggcgctg gcgtcgctgc
     4621 aagcgcagca ggctgctgat tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg
     4681 ggtgggagtt aaatcggtcg tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg
     4741 aagcaacgcg gaaggtggga cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc
     4801 tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa ggtaaggtta acggggtgaa
     4861 cggtgtggat taaagacggg atagcgataa ctaataacca aatcgactaa cctgttttag
     4921 caatagtctt taacaacaaa atagattaac caacctaatg aaaaacaaat gaatttagcc
     4981 aatcattaag ataaatcagc gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa
     5041 cagcctttcc ggtactctac ggcggtttta tcgtcctgta gagaaattat gatgtccacc
     5101 acgttattta aagatttcac cttcgaagcc gctcaccgct taccacacgt cccggaaggg
     5161 cataaatgtg gtcgcctgca cgggcattcc tttatggtgc gactggaaat taccggggaa
     5221 gtcgatccgc atacgggctg gattatcgat ttcgctgaac taaaagcggc gtttaaacca
     5281 acctacgagc gcctcgatca ccattatctc aatgatattc caggtctgga aaacccaacc
     5341 agcgaggttt tagcaaaatg gatttgggat caggttaaac ccgttgtgcc gctgttaagt
     5401 gcggtgatgg taaaagaaac ctgtaccgca ggttgtatct atcgcggcga atgataagag
     5461 tgtgtcggcg gtcaatttcc cttaagtaac gctatgttag ggtgttgtgt tctggatatc
     5521 tggggcatga catggaagac gactgcgaca ttattattat tggtgccggt attgcaggca
     5581 ccgcttgcgc gttacgctgc gcgcgagcgg gtttatccgt tttgttactg gaacgcgctg
     5641 aaatccccgg cagcaaaaat ctttccggcg ggcggttata tacccatgca ctcgcggaac
     5701 tcctcccgca atttcatctg accgcgcctc ttgaacgacg catcactcac gaaagccttt
     5761 ccctgttaac gccggatggc gcaacgacgt tttccagctt acagcccggc ggtgaatcct
     5821 ggagtgtatt acgtgcacgg ttcgatccgt ggctggttgc cgaagccgaa gaagaaggtg
     5881 tcgaatgcat ccccggagcg acggtggatg cactgtatga agaaaacggc agggtgtgtg
     5941 gtgtcatttg tggtgacgat attctccgtg cccgttatgt ggtgctggca gaaggtgcca
     6001 acagcgtcct ggctgagcgt cacgggttag tgactcgtcc tgctggtgaa gcgatggcgt
     6061 tggggatcaa agaagtgctg tcgctggaac catccgctat tgaagaacgt tttcatctgg
     6121 agaataacga aggcgcagcg ttgctgttca gcggcaggat ctgtgatgac ttacccggcg
     6181 gcgcatttct ttatactaat caacaaacgc tctcgttagg gattgtttgc ccgctctctt
     6241 cccttacgca aagtcgtgtt ccggcaagcg agctgctgac tcgctttaaa gcgcatccgg
     6301 cagtgcgccc gcttatcaaa aacacggaat cactggagta tggtgcgcat ctggtgccag
     6361 aaggtggctt gcacagtatg ccggtgcaat acgccggtaa cggctggctg ctggtgggcg
     6421 atgcgttgcg cagttgcgtc aataccggaa tttccgtgcg cggcatggat atggcgctga
     6481 ctggcgcgca ggcggcggca caaacgctga taagcgcctg ccagcaccgc gagccgcaaa
     6541 atctgtttgc gctttatcat cacaacgtag agcgcagcct gctatgggat gttctacaac
     6601 gttatcagca tgttccggcg cttttgcaac gccctggctg gtatcgggcg tggcctgcgt
     6661 taatgcagga tatttcccgc gatttatggg atcagggtga taaacctgtt ccaccgctgc
     6721 gccagttatt ctggcgtcat ttacgtcgtc atggcctgtg gcatctggcg ggcgatgtta
     6781 tcaggagtct gcgatgtctg tagcccataa tctctggcgc gttgctgatg cgccgcacat
     6841 tgttccggct gactccgttg agcgtcagat ggtacaacgg ttgattaacg cctgtccggc
     6901 aggtcttttt tcgctcacac cggaaggtga cttacgcgtt gattatcacg gctgcctgga
     6961 gtgtggcacc tgctgtttgc tgtgcgacga atccacacta caacagtggc gctatccgcc
     7021 ttccggattc ggcatcacct accgctttgg ataaataata aggataattt atgcccctct
     7081 tacacctgct ccgccagaat ccggtgattg ctgccgttaa agataatgcc agcctgcaac
     7141 tggcaatcga ctctgaatgc cagtttattt ccgtgttgta cggcaatatc tgcaccatca
     7201 gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc ttttattcat gttgatttgc
     7261 tggaaggcgc gtcaaataaa gaagtcgtga ttcagttttt gaaactggtg accgaggcgg
     7321 acggcattat cagtaccaaa gcctcaatgt tgaaagcggc aagagcggaa ggttttttct
     7381 gtattcatcg cctgtttatt gttgattcaa tttcgtttca caacattgat aagcaagttg
     7441 cgcaatcgaa tccggattgt attgagatcc tgccaggctg tatgcccaaa gtgctgggct
     7501 gggtgacaga gaaaatccgc caaccgctga ttgccggtgg gctggtgtgc gatgaagaag
     7561 atgcgcgtaa tgcgattaac gcgggtgtcg tggcgctttc caccacgaat accggggtct
     7621 ggacgttagc gaaaaaatta ctttgacggg ataatcgtaa ccaattgaat ttggtttgat
     7681 ttgtaggccg cacgccacat ccgacattca gcgcctgatg cgacgcttga cgcgtcttat
     7741 caggcctaca agtccccagg ccagaaccgt aggcaggatc atccggcaac acgttattgg
     7801 caatctgcgt gaatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg
     7861 ccaacatctg cctgtgaaaa taccgcagcg ctggcgtcat gattaatcgc caccacaaac
     7921 ttgctattgc gtacgcccgc cattaacgct gctgcgccag aagcgccaac aacaatacac
     7981 acctcaggtg ccagcagatg tccggaaata ccaatcactt tttcagcatc aacgccaccg
     8041 ttcatcactc gcgcccgact gtagcccact tcggctccca gcttttctgc cagcatagcg
     8101 atttcctggt tatctgcctc accgccctgc cccaccacca gcacccgtct ggcctcagct
     8161 aatgggtcac gggtaacatt tttcagatcc tctgtgctaa ccagccagtc gggaagcgcg
     8221 ccggggacaa tgttcagttg ctgcatacca gagggcaacg tggcgttttt cactgctccc
     8281 gcctgacgcg ccagggaaag gcatagcggg cgtttttctg tttgcagcgt cgccgttagc
     8341 gcatttcccc agtgggattt gcgcacgctt acggttggaa tatctaacga agtcacctgg
     8401 cagatgcttg caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgtt
     8461 cccggcggaa agagaacaac gtccgcaggc gttcgctgcc actgttccac caacgcatcc
     8521 agcacctgtt ccgccactac aggctgaggt tcaatctgcc agtgcgccag ggtacaaccg
     8581 ctgaaatcct gcgccgccag ccagcttgct attgcggcat tctcctgatt tattgtcaca
     8641 attgcgatat tcatggctgc atcctttggc gtaaatagtc ctgccatagc ttttgagctt
     8701 tttctgctac cgtttgccca tcaatcagcg tcgctccgcg tcgttgctct gctcttgcca
     8761 gttgcagaca ctgcatagct ggcatttcgg cagcaacagt tttacggata atttctgctt
     8821 tccccgcagc cattcgctgg cgcattcccg gtactggcaa ggccacttca ccgcactgac
     8881 gcacggcaat caccgcaggc aagcgaaccc ggcaacaacg caaaccatgt tcaatacgct
     8941 gttcgagggt gataaacagc gcgtcgagag tgaaacgctc cacctgggtg aagcagggcc
     9001 agcccagcat ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc
     9061 agccggtaat tatcagatcc agcggattct gatgctgcca ttcggcaata tggcgagcga
     9121 caaattccgg cgcaaagcgc agatctgccg ccgtctccag caatacagct tcctcaaacc
     9181 caagagccat gagatagcgc agccagtgca atgcccgttc atcccccatg ctcaacgcgg
     9241 ttaaagacat cggcgtaccg ttttttctct gcgccaacag cagcgcggca gcggcctgtt
     9301 catcagcacc gagtaaactt cgcagtagcg aaatatccgg tccgctttta ccctgagccg
     9361 ccgcctgcca ctctttttcc gccagcattc cggcatccgg ttcggcttta aacgctaaca
     9421 gaatgttcat tcgcacctcc tacatttccc gccgccacca gcgggagggc tttagtttct
     9481 ggtgcccata gccaggtcac aacaaaacca accaacaaca ctgtcgccag gagcaataag
     9541 gtgacttgca ttccccactg cgccagcacc cacggcagca ggccagtact taccgccgcg
     9601 ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa
     9661 ctttccgcag gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga
     9721 acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac aggccattac taccagcgtt
     9781 gccgccagca gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga
     9841 actaatccca gcagcgcgcc cacaattaac aacgcattaa gcatcaggct ggcagtcagc
     9901 gcatcttcca gaccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat
     9961 gggattacga ggcagacaaa gaagacgctg ttaaacgccg tgcgccgcca gtaacgcgaa
    10021 gagaacaagg ttttgatgtg tttatgggtc gccgttacca cttcatcgcc cagtaaaaca
    10081 tggggaccaa aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcgcagt
    10141 agccagcgtg gtgattctgg cgttccccag cgtaataacg taatcaacaa cgcgggcaga
    10201 gctgccgatg ccagtagcca gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg
    10261 gcaatacttg ccagcacata gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata
    10321 ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt gacctactga ataatcgcct
    10381 cccagaccaa tgccaatcaa aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa
    10441 aattgtaaaa acgaagcaag cgtaatcagc aaaaagctga aggtgaagat tttttgccga
    10501 ccaatatggt cggagatcca cccaagaacc aggctgccaa ggaacaaacc aaggagcgcc
    10561 gagccgccga tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagctgaata
    10621 atggcgtaac caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc
    10681 gcagcaatgc ggcagtgaaa acggttaagc ggtaaatcat ccattcgcac cggtgaagtg
    10741 ttcatcttta ccccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa
    10801 aggcaacgcc ccgtcatcgc ggaacgctgc ctgacacatt atttttcaat cggatagata
    10861 gtacccgtat tcataatgcc attaggatcg aactgctttt tcagcccttc cagcaacgcc
    10921 cacgcgctgc cgtgttccag tttgctccag tgaacgcgat gtttaccgat accgtggtgg
    10981 tgcaccatcg aaccgccgag gcgaatggtt tcttcacaga tgatcttgtt gagcggattg
    11041 tggtacttgt cgatttcctc ttccggctta cagtcaacga cgttgtaatc gtagacgaag
    11101 tacatgttgg tgccgttctg atagctatga gaggaatgac cgcccagcat ggtgatgtcg
    11161 tcggcgtgcg ggaactcagt acgaatacgg ttaataacgc tttcgtagat ttcgtggatg
    11221 cagctccagc agccggacac ttcagtggta aagcccatgt tgccggtttt gaggatctgc
    11281 acacgttcgg cagccacttt atccggtccc cagttcaggt tgttaaacca ggtttcgatc
    11341 agtttgctgt ccacgcgctg gcactgcggg tagcgggcaa cgatttccgc aatcccttcg
    11401 cccgtcgcct tcgcaatgcg agggttacct tcagccataa agatcagcac acattttccg
    11461 tctgcaaaat gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac
    11521 ggacgatacc cttccaccat gacttcacgc aggatgttga agccggtttt catgtcttcc
    11581 aggatatagc cgtagaagag gttgttttcc ggggtgaatt taaagatttt cactgttact
    11641 tcagtgatat agcaaagtgc accttcgtta ccgatgatga tgtggcgaat gtccgtgcca
    11701 gccgcgcggc gtggcacgtt tttaatgcgt gtgacggtgc catcagccaa tactgcttcc
    11761 agaccaacga ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg
    11821 gttgctacca ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg
    11881 taaccttttt cacgcaacgc attttccagc acttcctggg gaacgccaca ttgcgccgtc
    11941 gcctgcatat tctcaatatc aatattaatg atttgattca tggcggaacc gtcgagcacc
    12001 accgagtttt ctacaacagt ttccagccca ccttcagtgg cggaagcgcc ggtacgcggc
    12061 acaccgttaa ttttgtgcgc attcataaaa ttcagcacac gggagacttg ttctgtggaa
    12121 ccgagtttta cgaccgctgc cggaatcggc aaagtataaa taccatgaat atccggaaat
    12181 ttacgaaaac ggtcaatact gttcttcttt aatactgttt catcggtaat tacgcgatct
    12241 gcaccaacaa tttcctttag ttggtcgaca atcgctgcgc gagataaaga catagtaatt
    12301 ccttcctgat aataaaaatg gaataatcat taataagaga aaacagaaac caccgttatg
    12361 gattagcgca ctaaataacc accatcgacc actaataaat gcccattgac ataatttgaa
    12421 gccggacttg cgaggaatac agctgcgccc attaaatcct gagtatcgcc ccaacggttt
    12481 gccggaatat gatcaagaac gcgctgattg gtttctggat tactgcgtgt cgccagcgta
    12541 atatctgttg cataatagcc aggggcaata ccatttacct gaatattata ttgacctagt
    12601 tcatcacaat aagctttggt gaatccggca agagcatgtt tagtggcaga atatgcaggt
    12661 gaccattgtc cacctaagta agagaacaat gaacagatat taataatttt gccgcttttc
    12721 tgcgggatca taatttttgc agcttcatag cttaactcga atgcggcggt caggttcaca
    12781 tcaatcatcg gatcccagtc ggcacgaccg aagtccagca ccttattcag cttacaaata
    12841 ccggcattgt ttaccagaat atcaactgta ccgaaacgct cacagcaagc agcgataatc
    12901 ttctgcggcg cgccttctgc ggtgatatcc acctgcatga agtccacctc aacaccctgt
    12961 ttttcaatca tttcctttgt ttcgccgtta tctttgacga aactaggaat aaagatattt
    13021 gcgccagctt tggccaacgc catggcaaat gcctagccta aaccgctatt cccaccggta
    13081 acaattgcgg ttttaccttt cagtgagaaa aaatccattg agaacgcatt gagagattcg
    13141 attgacatag ttagctccaa tttccgtaag gcaaaaaaaa gaaaggtaac ttcccccgcg
    13201 agagggaagt tacctttctc attatctctg gtaactggag ataattttta gcataacggc
    13261 ataactgcta atgtgatcgt aatcacagtg tgatattcgt attgtcaaat agttttaaaa
    13321 agcagaagcg tgattactct cacacaacac ctctgctttc catgttattt cttattgcaa
    13381 ttactagaga cctgagaaaa gtaatccacc tgaaagggcg gcttactttt ctcttttttt
    13441 tgtctattac aaatgggaat acgatatgca acacaactca tatcgccgtt ggataaccct
    13501 cgcgataatt agttttagcg gcggcgttag ttttgacctg gcttatttac gttatattta
    13561 tcaaattccc atggcgaaat ttatgggatt cagcaatacc gagataggtt taataatgag
    13621 tacctttggt attgcggcta ttattcttta tgcccccagc ggcgttattg ccgataaatt
    13681 ttcacaccgc aaaatgatta cttccgcgat gatcattacc ggattactgg gtctgttaat
    13741 ggcaacgtat ccaccgctgt gggtaatgct ctgtattcag gtcgcctttg cgataacgac
    13801 gattttaatg ctgtggtcgg tgtcgattaa agccgcctcg ttgctgggcg atcatagtga
    13861 gcaagggaaa attatgggct ggatggaagg gctgcgcggc gtcggtgtaa tgtcgctggc
    13921 ggtgtttacc atgtgggtct tttctcgctt tgcaccggat gacagcgcca gcctgaaaac
    13981 agtcattatc atctacagtg tggtttacat cttgttgggg attctgtgct ggttttttgt
    14041 tagcgataac aacaacctgc gcagtgccaa taacgaagaa aaacagtcat tccagcttag
    14101 cgacatcctt gccgttttgc gtatcagcac cacctggtat tgcagcatgg tgatttttgg
    14161 tgtcttcaca atctacgcca ttctgagtta ctccaccaac tatctgaccg aaatgtacgg
    14221 catgtcgctg gtggcagcga gctacatggg gattgtgatc aacaaaatct tccgcgcgct
    14281 gtgcggccca cttggcggca ttatcaccac ctacagtaaa gtgaaatccc ctacccgcgt
    14341 gatccaaatc ctttccgtac tcggcctgct ggcgttaact gccctgctcg tcacgaactc
    14401 taacccgcaa tcggtcgcga tggggattgg cctgatttta ctgctgggat tcacctgtta
    14461 cgcctcacgc gggctgtact gggcctgccc tggcgaagtg agaacaccgt cttacattat
    14521 gggcaccacg gtaggtattt gttcggtgat tggattcctg ccggatgtct tcgtttaccc
    14581 gattatcggc cactggcaag acactctgcc cgcagcagaa gcctaccgca atatgtggct
    14641 gatgggaatg gcggcgcttg ccatggtgat tgtctttacc tttttgctgt tccaaaaaat
    14701 tcgtactgct gatagcgccc ccgcaatggc tagcagcaag taagccagga aaattacagg
    14761 agaaaaatca tgtcgaaaaa atacatcata gggattgatg gcggaagtca gagcacaaaa
    14821 gtggtgatgt acgatctgga aggtagtgtg gtttgcgaag gtaaaggctt attacagccg
    14881 atgcacacgc cttatgccga tactgcagaa catcctgacg acgatttatg ggcatcatta
    14941 tgttttgcag gtcacgattt gatgagccag tttgccggga ataaagaaga tattgttggc
    15001 atcggcctgg gctccatccg ttgctgccgt gcgttattga aagccgatgg cacgcctgct
    15061 gcgccgttga ttagctggca ggatgcacgc gttacacgcc cttacgaaca tacgaatcct
    15121 gacgtggcgt atgtcacctc tttttcgggt tatctgacgc atcgcttaac cggcgagttt
    15181 aaagacaata tcgccaacta ttttggtcag tggccggtgg attataagag ctgggcatgg
    15241 agcgaagatg ctgcggtaat ggataagttt aatatccccc gtcatatgct gtttgatgtg
    15301 caaatgcctg gcaccgtcct cggacatatc acaccacaag ccgcactggc gacacatttc
    15361 ccggcaggac tgccggttgt ttgtaccacc agtgataaac cggtagaagc tctgggggcc
    15421 ggattactgg atgatgaaac tgcggtaatt tctcttggca cttacatcgc attgatgatg
    15481 aacggcaaag cactgccgaa agatccggtg gcgtactggc cgattatgtc ttctattccg
    15541 caaacattgc tgtatgaagg ttacggtatt cgcaaaggta tgtggacggt gagctggctg
    15601 cgtgacatgt taggcgagtc gttaattcag gatgccaggg cgcaggatct ttcaccggaa
    15661 gatttactca acaagaaagc ctctagtgtg ccacctggct gtaatgggct gatgacggtg
    15721 ctggactggc tgaccaatcc gtgggaaccg tacaaacgcg ggattatgat cggctttgat
    15781 tccagcatgg attacgcatg gatatatcgt tcgatattgg aaagcgtggc gctaacgctg
    15841 aagaacaatt acgacaatat gtgtaatgaa atgaatcact ttgcgaagca tgtgatcatt
    15901 actggcggcg gttcgaacag cgatctgttt atgcaaattt ttgccgacgt gttcaacctt
    15961 ccggcacgcc gtaacgccat taacggttgt gcaagtctgg gagcagcgat taatacagcg
    16021 gtaggtctgg ggctataccc ggattacgca acggctgttg ataagatggt tcgcgtgaaa
    16081 gatatcttta taccgattga gagcaatgcc aaacgctacg acgcgatgaa taaaggcatt
    16141 ttcaaagacc taaccaaaca tactgatgtg atcctgaaaa aatcgtatga agtgatgcat
    16201 ggggaattgg ggaatgtgga ttcgatccag agctggtcga atgcgtgaat gaggtaggcc
    16261 gggcggaaaa ctgcccggcc tgaacatacc taaatggtta tccccgctga cgcggggaac
    16321 ataagttcga caacttaaaa aatgataaca agtccggttt atccccgctg atgcagggaa
    16381 cataataaaa actttaacta gttcggttta tccctgtttt tacaaggaat attaactaca
    16441 ctcgatgtct ttagaattaa agacaatacg aatcgcactt tatcaatttt cacttaaagt
    16501 atgaactgga tatctttatt cataagaact attcatcaac agcgtataga ggcggttatg
    16561 taaaaccact cattagcctc aaaacaaaga caaacttcca gatttattac ttaaccaatg
    16621 aataacaagc ttaaaaaata tactg
//
LOCUS       DAZGOB010000109        16451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGOB010000109 DAZGOB010000000
VERSION     DAZGOB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.109"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..248
                     /locus_tag="ACHLIF_003719"
     CDS             <1..248
                     /locus_tag="ACHLIF_003719"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO9607654.1"
                     /translation="ANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDAR
                     TAIPFEGERHNALDDARHQAKYVSAIWQKLIPNQADF"
     gene            310..579
                     /locus_tag="ACHLIF_003720"
     CDS             310..579
                     /locus_tag="ACHLIF_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9607655.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            548..1666
                     /locus_tag="ACHLIF_003721"
     CDS             548..1666
                     /locus_tag="ACHLIF_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO9607656.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1833..2627
                     /gene="potC"
                     /locus_tag="ACHLIF_003722"
     CDS             1833..2627
                     /gene="potC"
                     /locus_tag="ACHLIF_003722"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO9607657.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2624..3670
                     /gene="potD"
                     /locus_tag="ACHLIF_003723"
     CDS             2624..3670
                     /gene="potD"
                     /locus_tag="ACHLIF_003723"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO9607658.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            3728..4189
                     /locus_tag="ACHLIF_003724"
     CDS             3728..4189
                     /locus_tag="ACHLIF_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO9607659.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            4186..4974
                     /locus_tag="ACHLIF_003725"
     CDS             4186..4974
                     /locus_tag="ACHLIF_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO9607660.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(5113..5934)
                     /gene="cobB"
                     /locus_tag="ACHLIF_003726"
     CDS             complement(5113..5934)
                     /gene="cobB"
                     /locus_tag="ACHLIF_003726"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO9607661.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(5950..6861)
                     /gene="nagK"
                     /locus_tag="ACHLIF_003727"
     CDS             complement(5950..6861)
                     /gene="nagK"
                     /locus_tag="ACHLIF_003727"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO9607662.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(6890..8134)
                     /gene="lolE"
                     /locus_tag="ACHLIF_003728"
     CDS             complement(6890..8134)
                     /gene="lolE"
                     /locus_tag="ACHLIF_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO9607663.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(8134..8835)
                     /gene="lolD"
                     /locus_tag="ACHLIF_003729"
     CDS             complement(8134..8835)
                     /gene="lolD"
                     /locus_tag="ACHLIF_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO9607664.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(8828..10027)
                     /gene="lolC"
                     /locus_tag="ACHLIF_003730"
     CDS             complement(8828..10027)
                     /gene="lolC"
                     /locus_tag="ACHLIF_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO9607665.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10289..11362
                     /locus_tag="ACHLIF_003731"
     CDS             10289..11362
                     /locus_tag="ACHLIF_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9607666.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            11490..14936
                     /gene="mfd"
                     /locus_tag="ACHLIF_003732"
     CDS             11490..14936
                     /gene="mfd"
                     /locus_tag="ACHLIF_003732"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO9607667.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            15080..16042
                     /gene="ldtC"
                     /locus_tag="ACHLIF_003733"
     CDS             15080..16042
                     /gene="ldtC"
                     /locus_tag="ACHLIF_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO9607668.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(16125..16382)
                     /gene="bhsA"
                     /locus_tag="ACHLIF_003734"
                     /gene_synonym="ycfR"
     CDS             complement(16125..16382)
                     /gene="bhsA"
                     /locus_tag="ACHLIF_003734"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO9607669.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4161 a         4233 c         4134 g         3923 t
ORIGIN      
        1 gagccaactt cgacaacacg attttgcgcc gttcatacga acggcagggg atcccctgcc
       61 cgtggcgtta ctacaacgat cgcgatgtac gcacaatcgt tgagctgggg aaagccatag
      121 acttcgatgc cagaacggct attccattcg aaggtgagcg ccataatgca cttgatgacg
      181 ctcgtcacca ggcaaaatac gtttcagcta tctggcaaaa actgatcccg aatcaggctg
      241 atttttaatg ttcaaccgtc gccagttgtc gttgatattc tgcaactggc gcgttccgga
      301 gtgatagcca tgagcgaaca gtacctgata acgctcgacg agtggaaacc aaaacggttc
      361 agtctcccaa taacaaacac taccctggtg aaatacggaa aactaggata catcgttcca
      421 agaccacaaa aaattcgtgg gcgttggctg atagatcgcc gagcagtatt tgttgggcct
      481 ggtgaaacgg gaattgcgcc ggaaattcat actggcgatg atgatgcact gaaggagatt
      541 ttaactcatg tcaccgaggc cacgaaaaaa cagcactgac gtagccggtc tttacgaaaa
      601 gtttgatcgc agaactggca gagtttacta ccagtataaa aatcctgtga ctggaaaatt
      661 tcacggactc ggaacagaca aaggtaaggc agaaaaaatc gcttccacag ccaatcagcg
      721 aatagctgca gcagaagctg aatatttcat gcgcaaaatt gatgaaagtc cgtcagcaac
      781 aaaacgtcgg ggtatcagat taaaggcatg ggttgatcga tatctgaaaa tacaggacac
      841 gcgactgaaa aatggagata ttgcagctac aactcacaaa gaaaaaaccc gaatggctgc
      901 atacctggtt tcccgtctgg gaaaccaccc attgaaagaa ctggaagtaa gagactttgc
      961 attaatactg gatgagtggc tggataaaga catggtcagc acagcgagag taaatcgtgg
     1021 attatgggtt gatatttata aagaagcaca gcatgcaggg gaagttcctc ctggatggaa
     1081 tcctccggag gctacccgta aaccgatccc taaagtaacc agagccaggc tcaccatgga
     1141 agactggcaa aaaatttaca atgcaacgcc tgaaaaacac tttatccgta acgcaatgct
     1201 tcttgcgatt gttactggtc agcgccgtga tgacatttgc cacatgcgtt tttcagatgt
     1261 gtggaacgaa cacttgcata tcacccaggg aaaaaccgga atgcgtctgg cgttaccgct
     1321 tacactacgc tgtgatgcca ttgggataac gttaaaagaa gttattgatg ggtgccgaga
     1381 cagaatatta agtccatatc taatccatag tcggcaccag aaacaaccga agccgatgag
     1441 taaagacaac ctgagcgact actttgccaa agcacgggat ctggctggga taattccacc
     1501 agcaggaaaa actccgccaa catttcatga acaacgctct ctatcagaac ggctgtaccg
     1561 tgcacagggt attgatacaa aaacattact aggacataaa gtccaggcaa ccaccgatcg
     1621 ctataacgat actcgaggtc aggaatgggt taagttggtt atttgataaa caccatatgg
     1681 aatatccgtc accatctcag gaaaaaagtg ttgtaaaatg cgtggcctag ttttggggaa
     1741 acgttttgga gagattttgg agaaaggaaa aaattgaata aattcaaaat cctgaaagtg
     1801 tcccgcctta tgaataaaaa ggtggaactc gaatgatcgg tcgactgctt cgcggcggtt
     1861 ttatgaccgc tatctacgcg tacctgtata tcccaatcat tattttgatt gtgaactcct
     1921 ttaacagctc gcgctttggc atcaactggc agggttttac caccaaatgg tatagcctgc
     1981 tgatgaacaa cgacagcctg ttacaggcag cgcagcattc actaacaatg gcggtgtttt
     2041 cggcgacatt tgctacgctt atcggttcac tgacggcagt tgcgctgtac cgttatcgct
     2101 ttcgtggtaa gccgttcgtt agcggaatgc tgtttgtggt gatgatgtca ccagatatcg
     2161 tgatggcgat ttctctgctg gtgctgttta tgctgctggg tattcagctt ggcttctggt
     2221 cgctgctgtt ctcgcatatc accttctgcc tgccgtttgt ggtggtgacg gtgtattcgc
     2281 gcctgaaagg ttttgacgtg cggatgctgg aagcggcgaa agatctcggt gccagtgaat
     2341 ttaccattct gcgaaaaatc attctgccac tggcaatgcc tgcggtggcg gcgggctggg
     2401 tgttaagctt taccctgtcg atggacgatg tggtagtttc ttcgttcgtc accggaccaa
     2461 gttatgaaat tctgccgtta aaaatttatt cgatggtcaa agtcggcgta tcgccggaag
     2521 ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt gatggttatt gccagccagc
     2581 ttattgctcg tgataaaacg aaaggtaaca caggggacgt taaatgaaaa aatggtcacg
     2641 ccacctgctc gcggcgggtg ctctggcact gggcatgagc gccgctcacg ccgatgacaa
     2701 caacacgctg tatttctaca actggaccga gtacgtgccg ccaggactgc ttgaacagtt
     2761 caccaaagaa accggtatta aggttatcta ttcgacttac gagtcgaacg aaaccatgta
     2821 cgccaagctg aaaacgtaca aagacggtgc ctatgacctg gtggttcctt caacctatta
     2881 cgtcgataaa atgcgtaaag aagggatgat ccagaagatc gacaagtcga agttaacgaa
     2941 cttcagcaat ctcgatccag acatgctcaa caagccgttt gacccgaata acgattactc
     3001 cattccgtat atctggggtg cgacggcgat tggtgttaac ggtgatgcgg tggatccgaa
     3061 atctgtcacc agctgggccg atctgtggaa gcctgagtac aaaggcagcc tgctgttgac
     3121 cgacgatgcc cgtgaagtgt tccagatggc gctgcgtaag ctgggctact ccggtaacac
     3181 caccgatccg aaagagattg aagctgcata taacgagctg aaaaaactga tgccaaacgt
     3241 cgcagcgttt aactccgata acccagctaa cccgtacatg gaaggcgaag ttaacctcgg
     3301 catgatctgg aacggttctg ctttcgttgc acggcaggcg ggtacgccaa ttgacgtggt
     3361 gtggccgaaa gaaggcggca ttttctggat ggacagcctg gcgatcccgg caaatgccaa
     3421 aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg cgcccggatg tggcaaaaca
     3481 ggttgctgaa actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc tgttaagccc
     3541 ggaagtggcg aacgataaaa cgctctaccc ggatgctgaa accattaaaa atggtgaatg
     3601 gcagaatgac gttggcgcag ccagcagcat ttatgaagag tattatcaga agctgaaagc
     3661 aggacgttaa ttcagcaagc gggcggtggc attaccgccc gttttttatt tagcaaggat
     3721 gtggctaatg agtcaggaca gtaaagtgtt atcccgcgct tttctcggta tcggtctgat
     3781 attgctcgtt atttctgcaa tcatctttta ttaccatttt acttttacca aaagcgcggt
     3841 tcatacagaa ggaatcattg ttgatgccgt ttggtacaac aatcactcaa acgacgtcga
     3901 tgataatggt tcctggtatc cagtggttgc ctttcgccct acgccagatt acacgctaat
     3961 tttcaattcg aatatcggta gtgattttta tgaagatagc gaaggtgaca gagtcaatgt
     4021 ctattatccg ccagggcatc cagaacaagc cgaaataaac aatccctggg ttaatttttt
     4081 taaatggggc tttgttggca tagcaggcat tattttcggt agtgtcggtc tgataatcac
     4141 gctcccctcc accaaaaaat ctcgcaggaa acggaagtcc tgcccatgaa aaaattcatt
     4201 atactgctca gtttactgat actcctgcct ctggtagcaa ccagtaaacc attgattcca
     4261 ataatgaaga cactttttac ggatgttacc ggtactgtac cggatgctga agagatcgca
     4321 cgtaaagccg agctttttcg ccaacaaacg ggaattgcac cgtttattgt gattttaccc
     4381 gacattaata atgaagccag tctcagacaa aatggtaaag cgatgctggc gcatgcgtca
     4441 tcttcattga gtaatgtaaa aggaagtgtt ctgttactat ttactacccg cgaaccacgg
     4501 ttaattatga tcaccaacgg ccaggttgaa agtggtctgg acgataaaca tctcggcctt
     4561 ctgatagaaa atcacacgct ggcttattta aatgcagatc tctggtatca gggaatcaat
     4621 aatgcattgg ctgttctaca agcacagata ttaaaacaac cgacgccgcc actgacgtat
     4681 tatccgcatc caggacaaca gcatgaaaat gctccccccg gcagcaccaa cacattaggt
     4741 tttatcgcct gggctgcgac gtttatctta ttttcctgga tcttttgcta cacgactcgt
     4801 tttatttatg cgttaaaatt tgcagtggcg atgactattg ccaatatggg ttatcaggct
     4861 ttatgtttgt atatcgataa tagctttgcg attaccagaa tatcgccact ctgggcaggg
     4921 ctaattggtg tctgtacgtt tattgccgca ttgttattga cgagtaaacg ataaaagagg
     4981 ccggaaaagt gcgcgtgtat tatgccggat ggggcgcgaa cgccttatcc ggcccacaaa
     5041 acccgcaaat tcaattaatt gcgtcccctt gtaggcctga taagcgtagc gcatcaggca
     5101 atgcttcctg ctttataatc ccttcagcaa cttttcaaca aactcaggca ccacctggct
     5161 tgccgggccg taatatttct cggcaaattc attaccgacc tgacttggtt cgagattcag
     5221 ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg ccggataaac
     5281 atgcccggaa gtaccaatgg caatgaaaat atcagccatc gacaacgcca tataaatttc
     5341 atccatgccg agtggcattt cgccaaacca aacgacgtgc gggcgcaagg gtgccgggaa
     5401 ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga gaacctgacc
     5461 actttgcgaa caacgaactt tcagcaattc cccatgcata tgaatgacat tggtattacc
     5521 tgcgcgttca tgcaggttgt ctatattctg cgttaccagc aaaaagcgat cgccgagggc
     5581 gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttctg gctgctgcag
     5641 ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat cgaaaccttc
     5701 cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg cgcgaaaggt
     5761 acgaatacct gattccgcag aaatccctgc ccctgtcagt acgagtactc ttggtttttc
     5821 cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac gctcgcgcag
     5881 gcggcgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca gcatagcaac
     5941 ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc ccgcatcacc
     6001 gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac gaggcagcct
     6061 gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa tgacgaccag
     6121 gtcagggtca acaatggtca ggatatttcc cagacaaacc gctaataaat ccagataacg
     6181 ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa agcgcaataa tttcgggagc
     6241 ctgcaacggt tgatgataat agtgttgata cagccacgca aaaccgcgac cagacagata
     6301 attttcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat ccaaccccat
     6361 catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa tgtagctttt
     6421 accggtaatc ggtttgccgt tgaaaatcag cccgccgcca acgccggtgc cgagaatcaa
     6481 ccccatcacc agtggatatt gagtaaattc atcatcccag gcttctgaaa gggcaaaaca
     6541 gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt cggcacgcag
     6601 cggtttaccg ctggcagcgg ggacattggc ggcatacagc gtaccatctt ctgtttccgg
     6661 cattcccgga ataccgatgc cgacagaacc tttacagcca aaacgttggt cagcttcagc
     6721 taccagctca cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg tcggcacccg
     6781 cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt ttgttccacc
     6841 aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt tactggccgc
     6901 taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa cttgccaaaa
     6961 gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc aattccgatg
     7021 gcaggaaatc aataaaatag atatcgctgg agaggaactg atgaccgatc aacttttcaa
     7081 tccactcaat aatcggggta agttgcagtg aaaccaccac accgataatc accccacaca
     7141 ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga attaaaccat
     7201 ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc accgccatca
     7261 ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc gccagataca
     7321 taatggcgcg gatcatctgg atatcgcgat acatatagcc gtaagtacca atccaacttt
     7381 taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta ttggcgttga
     7441 aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca agatattgtt
     7501 gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc aactgcaaaa
     7561 taccggcaac gtgcaaacgc acacgttttg gctgcatcag tttatgctcg ggattcgagt
     7621 tggggatcat aatcgacacc caatccccct gcttcacttt cagcgcatcc gccacgcctt
     7681 tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccacgcatcg ccctggacaa
     7741 acgagggtaa tgcgctcaga cgctgttcct gttgcgggtt aacgcccttc acctggattg
     7801 cgcgaagatt cgcgccactt tccaccagcc cggtgaaatt gatatacggc gcggcagcgg
     7861 caatacccgg cactttttgc acgtgatcca gtgcttcctg ccagttagtc cacggctgat
     7921 ccaccgcctc gatttcacca tgcggcacca ccgccagaat acggttgttc agttcgcgtt
     7981 caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgccgagg gcaatgccaa
     8041 tggtagaaat aacggagatc agcgacacca tgccgccgcg ccgccgtccg cgactaaaac
     8101 gcaggccaat taataacgat aaaggcatcg ccattactcc gcccccatca ggctcagttc
     8161 cgccgtcaga cgaccatcac gcatttctaa ctggcggctc atacgtttcg ccagttgcag
     8221 gtcgtgagta accaccagga aggcggtgcc ctgcaagcga ttcaattccc caagcaactg
     8281 gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggttcatccg ccagtaccag
     8341 gcgcgggtta ttcaccagcg cacgggcaat cgccacacgc tggcgttcgc cgccagaaag
     8401 ttcagatggg cggtgattcg cacgatgatc cagccccacc gcttttaaca tctcaagtgc
     8461 acggctgttg atttcagcgg gctttttctt gccaatcagc agcggcatag ccacgttttc
     8521 cagggcggta aaatccggca gcaggtggtg aaactgataa ataaagccca gcttctggtt
     8581 gcgcagctct gctttcgccg ccgaagacag tttgctcatc ggttgaccgt taaagatcac
     8641 atcgccggag gttggcgtat ccagcccgcc caacaagtgc agcaaggtgc ttttaccgga
     8701 accagagcta ccgacgatcg ccatcatttc accttcgccg acgctgaaac tgacattgtg
     8761 cagcacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt cgcattgcaa
     8821 caggatctta ttcataacgt aaagcctcag cgggttgagt ggcggcagcg cgccatgaag
     8881 ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca atgacctgta
     8941 aaggttcgat agcgaccggc agcgccgcgc catcaagcag gacgccgatt atcggcatca
     9001 gattatttaa ctggctggca agcagtgcgc caagcgccgc accgaggatc gcaccgataa
     9061 tcccggcgct ggccccttgc accataaaga ccatcatgat ttgtcgcgga gttaagcctt
     9121 gcgtttgcag gatcgcgact tcgccctgct tctccattac catcagccct agtgaggtaa
     9181 taatgttaaa cgccgcaacg gcgacaatca ggctcagcag caagcccatc atattttttt
     9241 ccatgcgtac ggcctggaac agctcgcctt tacggtcacg ccagtcctgc catttgctgc
     9301 cttcaggcag tttttgctga cttaaagagt caactttcag cggctcatcc agccacaaac
     9361 gccagccggt aatattgcct gccggataac gcatcagacg cgaggcatcc tcaatattca
     9421 ccagcatttc atagccatcg acttcactgt tggcggcgaa agtgccaatc acattgaaca
     9481 ggcgctggct tggaatacgc cccatcggcg tgaactggct ggcagatggt accatcacgc
     9541 ggatttgatc accgcgatta acgcctagct gtgaggcaag ttgttcgccg aggatgacat
     9601 tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggt gtaagtggat
     9661 ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc cacgctgcgc gcgctttgca
     9721 gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt ttgactgccg
     9781 tttccgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt gcctgtggca
     9841 tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact gacaatactg
     9901 tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag acgaaacgac
     9961 cgaagcgatc cgctgcacgc ccacgcatgt aacgcaggcc aataaataga gcgacaggtt
    10021 ggtacatgaa atccgtctgg ttgctgtagc aaagacacga gtatataagc gaaagcaaga
    10081 tgggtaaacg tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc cccgtaaatc
    10141 aatcggctaa attgccatgt tttatcatca atcttgccgc ctgatcttaa tctgaaaaat
    10201 catccaatac agacagatcc ccattacacc tcgggtcata acccgcagga tcaaccgcat
    10261 gtaaattaaa aaagagcgtg ttgtcctgat gaaacaaaaa gagctatgga ttaaccagat
    10321 caaagggtta tgtatttgtc tggtggtgat ttatcactcg gtcattacct tttatccgca
    10381 tctgaccact tttcagcatc cgttatcgga agtcctgagc aaatgctgga tctatttcaa
    10441 tctttacctt gccccctttc gtatgccggt ttttttcttt atatccggct atttgattcg
    10501 ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacggatct ggaacatctt
    10561 ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg ttaagtgcgc tgaaccagtg
    10621 gctggcacct gagcgcgatt taagcaatgc ctccaatgcc gcttatgccg attccaccgg
    10681 tgagttcctg cacgggatga tcaccgccag caccagcttg tggtatctgt atgcgttaat
    10741 tgtctatttc gtggtatgta aaatttttag ccgtctggcg ctgccactat tcgccttgtt
    10801 tgtactgctg agtgtggcgg ttaatttcgt tcccacgccg tggtggggaa tgaacagtgt
    10861 gatccgcaat ttgctttatt acagccttgg cgcatggttt ggcgcaacaa taatgacctg
    10921 tgttaaagag gtgccgttgc gccgccatct gctgatggct tctttgctta ccgttctggc
    10981 ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg ctggtatcga ttgtggtgat
    11041 catgaagctg ttttatcaat acgagcaacg attcggtatg cgctccacca gcctgctgaa
    11101 tgtgattggc tccaacacca ttgctatcta caccacccat cgcattctgg ttgaaatatt
    11161 cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg tcgccgcaag tcgaactgac
    11221 actcctgctg gtttatccct ttgtcagttt gttcatctgt actgttgcgg gcttgctggt
    11281 aagaaaactt tcacagcgtg cattcagcga tctgttgttc tccccgcctt cactgcccgc
    11341 tgccgtcagt tactctcgtt aaaccatcgc cccagccggg gcgattcaat ttgctaaacc
    11401 atgtcgatta cggataatga acagcattgc ttatcagaga tatgccccca tatgttgagg
    11461 catatcctaa cgagaatctg acaaccgtta tgcctgaaca atatcgttat acgctgcccg
    11521 tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg cgcagcctgt gcaacgctgg
    11581 tagcggaaat tgccgaacgt cacgccggtc cggtggtact cattgcacca gatatgcaaa
    11641 atgctctgcg tttgcatgat gaaatcagcc agttcaccga tcagatggtg atgaatctgg
    11701 cggactggga aactcttccc tacgacagtt tttcgcctca tcaggacatt atctcctcgc
    11761 gcctttccac cctttaccag ctaccgacga tgcagcgtgg cgtactgatt gttccggtga
    11821 atacgcttat gcagcgcgtt tgcccacaca gttttctcca cggtcatgcg ctggtgatga
    11881 aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca actggacagc gccggttatc
    11941 gccatgttga ccaggtgatg gagcacggcg aatacgccac gcgcggcgcg ttgctggatc
    12001 tcttcccgat ggggagtgag ctgccttatc gtcttgattt ctttgatgat gaaatcgaca
    12061 gcctgcgggt gtttgacgtc gacagccagc gcacgctgga ggaagtagaa gcgatcaatc
    12121 tgctgcccgc gcacgaattt ccgaccgata aagcggcaat tgaactgttc cgcagccagt
    12181 ggcgcgatac cttcgaagtg aagcgcgatc cggaacatat ttaccagcaa gtgagtaaag
    12241 gcacattacc tgccgggatc gagtactggc agccattgtt cttcagcgaa ccactgccgc
    12301 cgctgttcag ttatttccct gccaatacct tgctcgtgaa tactggcgat ctggaaacca
    12361 gtgccgaacg tttccaggct gacacgctgg cgcgttttga gaatcgcggc gtcgatccga
    12421 tgcgcccgct gttgccacca caatcgctct ggctgcgggt ggacgagctc ttctcagagc
    12481 tgaaaaactg gccccgggtg cagctaaaaa ctgaacattt accgacaaaa gccgcgaatg
    12541 ccaatttagg tttccagaaa ctgccagacc tggccgttca ggcacaacaa aaagcgccgc
    12601 tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc ggtggtgttc tcggtagaaa
    12661 gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg aattaaaatt gctccgcaac
    12721 gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt ggcgctgccg
    12781 aacatggttt tgtcgataag gtgcgtaatc tggcgctgat ctgcgaaagc gatctgctcg
    12841 gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac catcaacccc gatacactga
    12901 tccgtaacct tgcggagctg catattggtc agccggtggt ccatctggag cacggtgtcg
    12961 ggcgctatgc cggaatgacc acgctcgaag cgggcggcat tactggcgag tatttgatgc
    13021 tcacctatgc caacgacgcc aaactgtatg ttccggtgtc gtcactgcat ctgattagcc
    13081 gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa acttggcggc gatgcgtggt
    13141 cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa ttgctggata
    13201 tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt gagcagtatc
    13261 agttgttctg cgacagcttc ccgtttgaaa ccacgccgga tcaggcgcag gccattaatg
    13321 cggtacttag cgacatgtgt cagccgctgg caatggatcg tctggtgtgc ggcgatgttg
    13381 gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct ggcagtagat aaccacaagc
    13441 aggtggcggt gttggtgcct accacccttc tcgcgcagca gcattacgac aacttccgcg
    13501 accgtttcgc caactggccg gtacgtatcg aaatgatctc ccgtttccgc agcgccaaag
    13561 agcagacgca aatccttgcg gaagtggcgg aagggaaaat cgatattctg atcggtacgc
    13621 acaaactgct gcaaagtgac gtcaagttta aagatttagg cctgctgatt gtcgatgaag
    13681 aacaccgctt cggggtgcgt cataaagagc gtattaaagc gatgcgcgcg aacgtggata
    13741 ttctgacgct tactgcaacg ccgatcccac gtacgctgaa tatggcaatg agcggaatgc
    13801 gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa acctttgtcc
    13861 gtgagtatga cagcctggtg gtccgggagg cgatcctgcg tgaaattttg cgcggaggac
    13921 aggtttatta tctctacaat gatgtggaaa acattcagaa agccgccgaa cggctggcag
    13981 aactggtgcc agaagcgcgg atcgccatcg gtcacgggca gatgcgcgag cgcgaactgg
    14041 aacgggtgat gaatgatttc catcatcaac gtttcaacgt gctggtttgt acaaccatta
    14101 tcgaaaccgg gatcgacatc ccgacagcca acaccattat cattgaacgc gcggatcact
    14161 tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg acgttcacat catcaggcat
    14221 atgcatggtt gctgacgccg catccaaaag cgatgactac cgatgcacaa aaacgtcttg
    14281 aagcgattgc ctcgctggaa gatctcgggg caggttttgc gctggcaacg cacgatctgg
    14341 agattcgcgg cgcgggtgaa ctgcttggcg aagaacaaag cggctcaatg gaaaccatcg
    14401 gtttctcgct gtatatggag ttgctggaaa acgccgtcga tgcactgaaa gccggacgcg
    14461 agccgtcgct ggaagatctc accagccagc aaacagaagt cgagctgcgg atgccgtcgc
    14521 tattgccaga tgatttcatt cctgacgtga atacgcgttt gtcgttctat aaacgtattg
    14581 ccagcgccaa aacggaaaac gaactggaag agatcaaagt cgagcttatc gatcgcttcg
    14641 gcctgctgcc ggatccggcg cgtaccctgc tggatgttgc ccgtctgcgc cagcaagcgc
    14701 agaaactggg gatcaggaag ctggaaggta atgagaaagg cggggtgatc gaatttgccg
    14761 agaagaatca cgttaatccg gcctggttga ttggtttgct gcaaaaacag ccgcagcatt
    14821 accgccttga tggtccgacg cgcctgaaat ttattcagga tttgagtgag cggaaaacgc
    14881 gtatcgaatg ggtacgccag tttatgcgtg aactggaaga gaacgcgatc gcttaatggt
    14941 aaatcggggg cgtttctgcg cccccatctg caacatttac aaattctttg cacttccctg
    15001 cactatccga cactgtcacc atccataatt caggcttatc tgtttattac aataacctta
    15061 tatttattat ggatttttgg tgatgatcaa aacgcgtttt tctcgctggc taacgttttt
    15121 tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa gccaacacct ggccgctgcc
    15181 gccagcgggc agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa atgacggtgg
    15241 ttctctggaa gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt tacaggctaa
    15301 ccctggcgtt gatccttacg taccgcgcgc gggtagcgtg ttaacgatcc cgttgcaaac
    15361 cctacttcca gatgcgccgc gcgaaggcat tgtgatcaat attgcggagc tgcgtctcta
    15421 ttactacccg ccgggtaaaa attcggtaac cgtgtatcca ataggtattg gtcagttagg
    15481 tggtgacacg ctgacaccga caatggtgac caccgtttca gacaaacgtg caaacccaac
    15541 ctggacgcca acggcaaaca tccgcgcccg ttataaagca cagggaattg agttgcctgc
    15601 ggtagtgccg gctggaccgg ataacccaat gggccatcat gcgattcgtc tggcggccta
    15661 tggcggcgtt tatttgcttc acggtacgaa cgccgatttc ggcattggca tgcgggtaag
    15721 ttctggctgt attcgtctgc gggatgacga tatcaaaaca ctctttagcc aggtcacccc
    15781 aggcaccaaa gtgaatatca tcaacactcc gataaaagtc tctgccgaac caaacggtgc
    15841 gcgtctggtt gaagtacatc agccgctgtc agagaagatt gatgacgatc cgcagctgct
    15901 gccaattacg ctgaatagcg caatgcaatc atttaaagat gcagcacaaa ctgacgctga
    15961 agtgatgcaa catgtgatgg atgtccgttc cgggatgccg gtggatgtcc gccgtcatca
    16021 agtgagccca caaactctgt aaatcctgga taaaaaaaat ccccgcgaca cggcatgtcg
    16081 cggggaaaaa atcagcagta gcaggcatta atgagggtta atgcttattt ataaattact
    16141 gctgttccat ggagggtatt cggaccggtt acagaagtaa tacggaaaga ttttgcgccc
    16201 atctcatccg ctttttgcgc cagctgctct tccagcgatc ccagatttgt ccccgcgtta
    16261 gcactgattg taccgacttt ttgttggcct tctggcgttg actgaacttc gacagccgca
    16321 aagctggcaa atgacatgga gcttaaaatc gccgcagcga tgagggtttt tacgtttttc
    16381 ataatagtgg ccttatgcag atgaatgacg tcgcaagcaa ttacttaacg atcgataact
    16441 aaatgataga t
//
LOCUS       DAZGOB010000110        16374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGOB010000110 DAZGOB010000000
VERSION     DAZGOB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.110"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            139..969
                     /locus_tag="ACHLIF_003735"
     CDS             139..969
                     /locus_tag="ACHLIF_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9607670.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            1001..1936
                     /locus_tag="ACHLIF_003736"
     CDS             1001..1936
                     /locus_tag="ACHLIF_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9607671.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            2029..2271
                     /gene="yhdT"
                     /locus_tag="ACHLIF_003737"
     CDS             2029..2271
                     /gene="yhdT"
                     /locus_tag="ACHLIF_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO9607672.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2261..3712
                     /gene="panF"
                     /locus_tag="ACHLIF_003738"
     CDS             2261..3712
                     /gene="panF"
                     /locus_tag="ACHLIF_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO9607673.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3724..4605
                     /gene="prmA"
                     /locus_tag="ACHLIF_003739"
     CDS             3724..4605
                     /gene="prmA"
                     /locus_tag="ACHLIF_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO9607674.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4934..5899
                     /gene="dusB"
                     /locus_tag="ACHLIF_003740"
     CDS             4934..5899
                     /gene="dusB"
                     /locus_tag="ACHLIF_003740"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO9607675.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5925..6221
                     /gene="fis"
                     /locus_tag="ACHLIF_003741"
     CDS             5925..6221
                     /gene="fis"
                     /locus_tag="ACHLIF_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO9607676.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6307..7191
                     /gene="yhdJ"
                     /locus_tag="ACHLIF_003742"
     CDS             6307..7191
                     /gene="yhdJ"
                     /locus_tag="ACHLIF_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO9607677.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7275..7454
                     /gene="yhdU"
                     /locus_tag="ACHLIF_003743"
     CDS             7275..7454
                     /gene="yhdU"
                     /locus_tag="ACHLIF_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO9607678.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7457..8119)
                     /gene="acrS"
                     /locus_tag="ACHLIF_003744"
     CDS             complement(7457..8119)
                     /gene="acrS"
                     /locus_tag="ACHLIF_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO9607679.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            8518..9675
                     /gene="acrE"
                     /locus_tag="ACHLIF_003745"
     CDS             8518..9675
                     /gene="acrE"
                     /locus_tag="ACHLIF_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO9607680.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            9687..12791
                     /gene="acrF"
                     /locus_tag="ACHLIF_003746"
     CDS             9687..12791
                     /gene="acrF"
                     /locus_tag="ACHLIF_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO9607681.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            13044..13265
                     /locus_tag="ACHLIF_003747"
     CDS             13044..13265
                     /locus_tag="ACHLIF_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607682.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            13696..14721
                     /locus_tag="ACHLIF_003748"
     CDS             13696..14721
                     /locus_tag="ACHLIF_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9607683.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            14789..15178
                     /locus_tag="ACHLIF_003749"
                     /pseudo
     CDS             14789..15178
                     /locus_tag="ACHLIF_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            15233..>16374
                     /locus_tag="ACHLIF_003750"
     CDS             join(15233..15521,15521..>16374)
                     /locus_tag="ACHLIF_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612098.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFO9607684.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFCEGGV
                     RPPLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     REIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRL"
     misc_feature    15514..15630
                     /locus_tag="ACHLIF_003750"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT         4025 a         3914 c         4192 g         4243 t
ORIGIN      
        1 ttcatttatt tctcaatgaa ttaatctatt tttgttgcga tttttggccc atgattttct
       61 tttcgaattg tcgatattat tgaaggataa ctttcaaaga aaaatacaag gggctatagt
      121 gttacgcatt aaaggccaat gaagagtgtt aacgtgccga gaaaatcgtc taacctactg
      181 aaacgcagat taaaaattgc agaaattgtt gccgcccaag gtgaaatcaa agttgatgac
      241 ttatctgcgc aacttggtgt ctctggcgtg acgatccgcg gcgatctcag ttatctggag
      301 caacagggat atttaaagcg atcatttggc ggtgctattg cgacaccact ctcctctgaa
      361 ccagaagcga tacaaccgat cgcgccattg tctctggcac aacaaatgga aatagcacga
      421 cattgcgcac ggttaattcg cgatcgtgac acgctatttc ttggacatgg gacaatctgc
      481 cgtaagatca ttccattgct cagcgaagtg aaaaaactgc gtctgatcac caacgatccc
      541 gaacatgcgc tgctggcaaa tcagtttatt gatggcgaaa taatactggc tggcagtgaa
      601 atgctgcgcc ccgacaccgc actggccgga aaaccgcttg aacaggcact tcagcatttc
      661 accattacac atagcattct ggaaatcagc catctggatg ctgatggtac tttaaatatc
      721 aacgttccca gactggccgc tgcctggcag ctctgtttcg atcatgccca gaataagacg
      781 gttatcgttg ccgccgatcc tgcgccatca acggctgcta tcgggcattt gcatcaggca
      841 gaaaatgtga tcgtcagccg cagcattaac gaacaatatc agcaacaatt gcagtcggct
      901 gatttcgtta tcctttatac cagtaatgag tgctttacct ggtccaatcg cgaaaggctt
      961 caggaatgag aggggttttg tgacaataac tatctgcgct atgggcgaat tgctggccga
     1021 gtttttgtcc cgcaacccac atcaaaaatt cactcagcct ggggagttta tcgggccatt
     1081 tcccagcggt gcgccagcaa tttttgctgc tcaggtggca aaactgtccc atcgggccat
     1141 cttcttcgga tgtgttggta atgatgattt tggccgactc attatagagc gcctccgtca
     1201 tgaaggtgtc attaccgatg ggatccatgt tatgaacaat gccgtcacag gtacggcgtt
     1261 cgtgagttat caaaatcccc agcagcgcga tttcgtcttt aatatcccta acagcgcctg
     1321 cggcttgttt actgctgagc acattgataa ggatctgcta aaacagtgta accatctcca
     1381 tattgtgggc tcatcgttgt tctcatttcg catgatcgat gtcatgcgta aagcaataac
     1441 gacgatcaaa tcggctggcg gcaccgtttc tttcgatccc aatattcgca aagagatgct
     1501 gagcattcct gaaatggcgc aggctctcga ttatttgatt gaatatacgg atattttcat
     1561 ccccagcgaa agcgaactcc ctttcttcgc gcgtcacaaa aatctgtcag aagaacagat
     1621 tgttagcgat cttctccacg gcggcgtaaa acatgtggcg ataaaacgcg cccagcgtgg
     1681 ggccagctat tacaagctta aaaacggtac attacacgcc cagcatgtta caggccacga
     1741 tatcgaaatt atcgatccaa cgggtgcagg cgactgcttt ggcgcaacgt ttatcactct
     1801 tttcttatcc ggtttcccgg cacataaggc actgcaatat gcaaatgcca gcggcgcgct
     1861 cgccgtaatg cggcaaggtc cgatggaagg gatatcctca ctggctgaca ttgaagactt
     1921 tttgcagcag cactgaacgg caattttctg tcagattatt ttccctgcct gaagagttgc
     1981 cgtagaattg cccgtttctc gtatcccccc atgaggagga acccttccgt ggacactcgt
     2041 tttgttcagg cccataaaga ggcgcgctgg gcgctggggc tgaccctttt gtatctggca
     2101 gtttggttag tagccgctta cttacctggc gttgcccccg gttttaccgg ctttccgcgc
     2161 tggtttgaga tggcctgcat cctgacgccg ctgctgttta ttggactgtg ctgggcgatg
     2221 gtgaaattta tctatcgcga tatcccactg gaggatgacg atgcagcttg aagtaattct
     2281 accgctggtc gcctatctgg tggtggtgtt cggtatctcg gtttatgcga tgcgtaaacg
     2341 gagcaccggc accttcctta atgagtattt cctcggcagc cgctctatgg gcggtattgt
     2401 gctggcgatg acgctcaccg cgacctatat cagtgccagt tcgtttatcg gcgggccagg
     2461 agctgcttat aaatacgggc tgggctgggt attgctggcg atgattcagc ttcctgcagt
     2521 ctggctttca ctcggtattc tcggcaagaa gtttgcgatt cttgcgcgcc gctacaatgc
     2581 agtgacgctg aacgatatgc tgtttgcccg ctaccagagt cgtcttctgg tgtggctggc
     2641 gagtttgagt ttgctggttg cgttcgttgg tgcgatgacc gtgcagttta tcggcggtgc
     2701 gcgcctgctg gaaaccgcgg cgggtattcc ttatgaaacc gggctgctga tttttggtat
     2761 cagcattgcg ttatataccg cctttggtgg ctttcgcgcc agcgtgctga acgacaccat
     2821 gcaagggctt gtgatgctga ttggcaccgt tgtgctgctt attggcgtgg tacatgccgc
     2881 tggcggctta agcaacgcag tacagacctt gcaaaccatc gatccgcaac tggttacgcc
     2941 acaaggcgct gacgatattc tgtcgcctgc ctttatgacg tcgttctggg tactggtgtg
     3001 ctttggcgtg attggcctgc cgcatactgc ggtgcgctgt atctcttata aagacagcaa
     3061 agcagtacac cgggggatca tcatcggtac gattgtagtc gcaatcctga tgttcggtat
     3121 gcacctggcc ggagcgttag gtcgtgcagt gatccccgat ctcaccgtac cggatctggt
     3181 gatcccgacg ttaatggtaa aagtgctgcc accgtttgct gccggaatct tcctggctgc
     3241 acctatggct gcgatcatgt cgacgatcaa tgcccagttg ctgcaaagtt ccgctacgat
     3301 cattaaagat ctctatctga atatccgtcc ggatcaaatg caaaacgaga cgcgtctgaa
     3361 gcggatgtcg gcggtaatta cgttagttct cggcgcgttg ctgctgcttg ccgcctggaa
     3421 gccgccagaa atgatcatct ggctgaattt gctggccttc ggtgggctgg aagccgtttt
     3481 cctgtggccg ctggtgctgg gtctttactg ggaacgcgcc aacgccaaag gcgcgctaag
     3541 tgcgatgatc gttggcggcg tgctgtatgc cgtactcgcg acgctgaata ttcagtacct
     3601 gggcttccac cctattgtgc cctcgttact actaagtttg ctggctttcc tggtcggaaa
     3661 ccgtttcggt acatccgtcc cgcaagctac cgttttgact actgataaat aaagagtttt
     3721 gccatgcctt ggatccaact gaaactgaac accaccggcg cgaacgcgga agatcttagc
     3781 gatgcgctga tggaagcggg tgccgtttct atcacttttc aggataccca cgatacgcca
     3841 gtatttgagc cgctgccggg cgaaacgcgc ctgtggggcg acaccgatgt gattggtctg
     3901 ttcgacgctg aaaccgatat gaacgacgtg gtggcgattc tggaaaacca tccgctgctc
     3961 ggcgcaggct tcgcgcataa aatcgaacaa ctagaagata aagactggga gcgcgaatgg
     4021 atggataatt tccacccgat gcgctttggt gaacgactgt ggatctgccc tagctggcgt
     4081 gatgtgccgg acgaaaacgc cgtcaacgtg atgttagatc cagggctggc gtttggtacg
     4141 ggtacccatc caaccacctc tctgtgcctg caatggctcg acagcctcga tttaaccggt
     4201 aaaacagtca tcgactttgg ctgtggttcc ggcattctgg cgatcgcggc gctgaaactg
     4261 ggtgcagcaa aagccattgg tattgatatc gatccgcagg cgattcaggc cagccgcgac
     4321 aacgccgaac gtaatggcgt ttctgaccgt ctggaactct acttaccgaa agatcagcca
     4381 gaagaaatga aagccgacgt ggtggtcgct aacatccttg caggcccatt acgtgaactg
     4441 gcaccgttaa tcagcgtcct gccggtttca ggcggtttgc tgggcctttc cggtattctg
     4501 gcaagccagg cagagagcgt ttgtgaagct tatgccgata gcttcgcact ggacccggtc
     4561 gtggaaaaag aagagtggtg ccgtattacc ggtcgtaaga attaaccttc gcatcgccgt
     4621 agggtgacgc ggggcgaagt gcgagcaagc tcacaaaagg cacgtaaatt tgccggttat
     4681 ttacgcaaat ttgcgtgcca aaattttcat tcataaagaa aaattgagaa cttactcaaa
     4741 tttctttgag tgtaaatttt agtcactatt ttctaatatg atgattttta tgagtaatta
     4801 tcgcaccacg ctcattttaa atgcaattct ttgatccatc tcagaggatt ggtcaaagtt
     4861 tggcctttca tctcgtgcaa aaaatgcgta atatacgccg ccttgcagtc acagtatggt
     4921 catttcttaa ctcatgcgca tcggacaata tcagctcaga aatcgcctga tcgcagcgcc
     4981 catggctggc attacagaca gaccttttcg gacgttgtgc tacgagatgg gagccggatt
     5041 gacagtatcc gagatgatgt cttctaaccc acaggtttgg gaaagcgaca aatctcgttt
     5101 acggatggtg cacattgatg aacccggtat tcgcaccgtg caaattgctg gtagcgatcc
     5161 gaaagaaatg gcagatgcag cacgtattaa cgtggaaagc ggtgcccaga ttattgatat
     5221 caatatgggt tgcccggcta aaaaagtgaa tcgcaagctc gcaggttcag ccctcttgca
     5281 gtacccggat gtcgttaaat cgatccttac cgaggtcgtc aatgcagtgg acgttcctgt
     5341 taccctgaag attcgcaccg gctgggcacc ggaacaccgt aactgcgaag agattgccca
     5401 actggctgaa gactgtggca ttcaggctct gaccattcat ggccgtacac gcgcctgttt
     5461 gttcaatgga gaagctgagt acgacagtat tcgggcagtt aagcagaaag tttccattcc
     5521 ggttatcgcg aatggcgaca ttactgaccc gcttaaagcc agagctgtgc tcgactatac
     5581 aggggcggat gccctgatga taggccgcgc agctcaggga agaccctgga tctttcggga
     5641 aatccagcat tatctggaca ctggggagtt gctgcccccg ctgcctttgg cagaggttaa
     5701 gcgcttgctt tgcgcgcacg ttcgggaact gcatgacttt tatggtccgg caaaagggta
     5761 ccgaattgca cgtaaacacg tttcctggta tctccaggaa cacgctccaa atgaccagtt
     5821 tcggcgcaca ttcaacgcca ttgaggatgc cagcgaacag ctggaggcgt tggaggcata
     5881 cttcgaaaat tttgcgtaaa cagaaataaa gagctgacag aactatgttc gaacaacgcg
     5941 taaattctga cgtactgacc gtttctaccg ttaactctca ggatcaggta acccaaaaac
     6001 ccctgcgtga ctcggttaaa caggcactga agaactattt tgctcaactg aatggtcagg
     6061 atgtgaatga cctctatgag ctggtactgg ctgaagtaga acagcccctg ttggacatgg
     6121 tgatgcaata cacccgtggt aaccagaccc gtgctgcgct gatgatgggc atcaaccgtg
     6181 gtacgctgcg taaaaaattg aaaaaatacg gcatgaacta attcaggtta gctaaatgct
     6241 tgattaaaaa ggcgctactc ggcatgggga agcgcctttt ttataggtgt cacaaaggga
     6301 gtgaccatga gaacaggatg tgaaccgacc cggtttggta atgaagctaa gaccattatt
     6361 cacggtgatg cgcttgccga acttaaaaag atccccgccg aaagtgtcga tctgatcttt
     6421 gccgacccac catataacat cggtaaaaat tttgatggtc tgatcgaagc ctggaaagaa
     6481 gatctgttta tcgactggct gtttgaagtg attgcagagt gccaccgcgt tctgaaaaag
     6541 cagggcagca tgtacatcat gaacagcacg gaaaacatgc cctttatcga tctccagtgc
     6601 cgcaagcttt ttaccatcaa aagtcgcatc gtctggtcat atgacagttc tggagtacag
     6661 gcgaaaaaac actacggctc catgtacgaa cccatcctga tgatggtgaa agacgcaaag
     6721 aactacacat tcaacggtga tgctattctg gtagaagcca aaaccggatc gcagcgcgcg
     6781 ttgatcgatt atcgcaaaaa tcctccacag ccatacaatc atcaaaaagt accgggtaac
     6841 gtctgggatt ttccgcgcgt gcgttatttg atggatgaat atgagaacca cccgacgcaa
     6901 aaaccggaag ccttactgaa acgcattatt ctcgcctctt ccaacccagg cgatatcgtt
     6961 ctcgacccgt ttgccggtag ctttactacc ggtgccgtag ccgtctccag cgggcgaaaa
     7021 ttcattggta ttgagatcaa cagcgagtac atcaaaatgg ggcttcgacg gctggatgtc
     7081 gcgtcgcatt actctgcgga agaactggcg aaagtgaaaa aaagaaagac cggcaacctg
     7141 tcaaaacgaa gccggttaag cgaagttgac cccgatctca ttgcaaagta aaggaagtgt
     7201 aagcctggtt ttcagattat tcatttcgtg tatatttcct gccagacttg gttaaacata
     7261 cacaggctca ggtaatgatt cgcaagtatt ggtggctcgt cgttttcgct gtcttcgttt
     7321 ttctgtttga tactttactg atgcagtgga ttgaactgct ggcaacagaa acagacaaat
     7381 gccgcaatat gaactcagtt aatccactaa aactggttaa ctgtgacgaa ctgaattttc
     7441 aggacagaat gtgaatttac atgacactta attcattcgt ttgatgaatt aatttcgtta
     7501 tgttttcatc tggcatgaac attcttaata cgttatcgac cagagcgggg gcttgtttat
     7561 aaagatcata acccgccata ttcattaacc agttttgaac aattccgctg aaggcaccat
     7621 taataataat catcacaaca tctaaatcga ggttatttgc tacacaacct tgttgctgac
     7681 acgcctgcaa tacttcgcgg agagtctgcg gattgaagcc catcttttcg cgtatcactc
     7741 cctcggccag catctcatca ttaaattcac atttgtgata taagattttc agcaacgcct
     7801 gctggcgggg aattttggca atatattgca agccgacaat caatttttca cgcaattgtt
     7861 gaaacgggtc atgctctaat ccagccgtca agtgttcctg gattaactcc cgcaatgaag
     7921 gctgttgcaa ccacatctca ttaaacagtt gagtcttgtt ttcgaagtgc cagtagatag
     7981 cgccacgcgt aacgttagca gcgtcggcaa tgtcgttgag tgtcgtcttg cttacgccat
     8041 gctgcgcaaa ctgggcgatg gcagtttcaa tcagttcttg ccgggtcttc agagcttcgg
     8101 ctttggttct ttttgccatg attaattatt caggaaataa atatattcga cacagagtga
     8161 gaaaatagcg aaggttaatc tatcacctaa tgtgtattta tacgagaggc taatattgag
     8221 ttgctataaa tcgtcaaata aataatatat attatttacc taaaatacat tcgccacatg
     8281 aatatatatt tcaatttacg aggttttaat tctgcctctt tcaacccgcg tcaaaataaa
     8341 acagtagaat attaatcttt ttttgtgttt atgtgccttg agacgccagt attcataact
     8401 attccttaca tcgacgaatg ataatttgta ggatagcaaa ctgtattttt ctttctgcga
     8461 gttaacgcgt tacctttctg ggtaaataac gcgcttttgg ttttttgagg aatagtaatg
     8521 acgaaacatg ccaggttttt cctcctgccc tcctttattc tgatctccgc ggctttaatc
     8581 gccggttgta acgataaggg agaagagaaa gctcacgtcg gtgaaccgca ggttaccgtt
     8641 catattgtga aaacggcccc gttagaagtt aagactgaat taccaggccg caccaatgct
     8701 taccgtatag ccgaagttcg cccacaggtt agcgggatcg tactgaatcg taatttcact
     8761 gaaggcagcg atgtacaagc aggccagtcc ctgtaccaga tcgatcccgc gacctatcag
     8821 gcaaattatg acagcgcgaa aggcgaactg gcgaaaagtg aagccgccgc cgccatcgcg
     8881 catttgacgg taaaacgtta cgttccgctc gtgggtacga aatacatcag ccagcaggag
     8941 tacgaccagg ccattgctga tgctcgccag gccgatgccg ccgtgattgc cgcaaaagcc
     9001 acagtcgaaa gcgctcgcat caatctcgct tataccaaag tgactgcgcc aattagcgga
     9061 cgtatcggca aatcgaccgt gaccgaaggc gctcttgtca ctaatgggca aacgactgaa
     9121 ctggcgactg tccagcagct cgatcctatc tacgttgatg tgacccaatc cagcaacgat
     9181 tttatgaggc tgaagcaatc cgtagagcaa ggaaatttgc ataaggaaaa cgccaccagc
     9241 aacgtagagt tggtcatgga aaacggtcaa acctatcccc tgaaaggtac gctgcaattc
     9301 tctgatgtga ccgttgatga aagcaccggc tccataaccc tacgtgctgt cttccctaac
     9361 ccgcaacata cgcttttgcc gggtatgttt gtgcgtgcac ggattgatga aggcgtccaa
     9421 cctgacgcca ttcttatccc gcaacaaggc gttagccgca caccgcgtgg tgatgcaacc
     9481 gtgctgattg ttaacgataa aagtcaggtg gaagcgcgcc ctgtcgttgc cagtcaggcg
     9541 attggcgata aatggttgat tagcgaaggt ctgaaatctg gcgatcaagt cattgtcagc
     9601 ggcctgcaaa aagcgcgtcc gggagagcag gttaaagcca ctaccgatac ccccgcagat
     9661 actgcatcga agtaaggtaa tctgacatgg caaacttttt tattcgacga ccgatatttg
     9721 catgggtgct ggccattatt ctgatgatgg cgggcgcact ggcgatccta caattgcccg
     9781 tcgctcagta tccaaccatt gcaccgcctg cggtttctgt ttcggcaaac tatccaggtg
     9841 ctgatgcgca gaccgtgcag gatacggtga cgcaggttat cgaacagaat atgaatggta
     9901 tcgataacct gatgtatatg tcctccacca gcgattccgc cggtagcgtg acaattaccc
     9961 ttaccttcca gtccgggacc gatcctgata tcgcgcaagt gcaggtgcag aacaaactcc
    10021 agctcgccac gccgttgctg ccgcaggagg ttcagcagca ggggatcagt gttgaaaagt
    10081 ccagtagcag ctatttgatg gtggcgggct ttgtctctga taacccagac accacacagg
    10141 acgatatctc ggactatgtg gcctctaacg ttaaagatac gcttagccgt ctgaatggcg
    10201 tcggtgacgt acagcttttc ggcgcacagt atgcgatgcg tatctggctg gacgccgatc
    10261 tgctaaacaa atataaactg acaccggttg atgtgattaa ccagttgaag gtacagaacg
    10321 atcagatcgc tgccggacag ttgggcggaa cgccagcgtt accagggcaa cagttgaatg
    10381 cctcgattat tgctcaaacg cggttaaaaa atccggaaga attcggcaaa gtgaccctgc
    10441 gcgtaaacag tgacggctcg gtggtacgcc tgaaagatgt cgcacgggtt gaacttggcg
    10501 gtgaaaacta taacgttatc gctcgtatca acggaaaacc ggcggcgggc ctggggatta
    10561 agctggcaac cggcgcgaat gctctcgata ccgcgaaagc cattaaggca aaactggcgg
    10621 aattacagcc attcttcccg cagggaatga aggttctcta cccttatgac accacgccat
    10681 tcgtccagct ttctattcac gaagtggtaa aaacgctgtt cgaagccatt atgctggtgt
    10741 tcctggtgat gtatctgttc ttgcagaata tgcgagcaac gctgatcccc accattgcgg
    10801 tacccgtggt gttgttaggg acgtttgcca tcctcgccgc ttttggttac tccatcaaca
    10861 cactaacgat gttcgggatg gtgcttgcca tcgggctgct cgtcgatgat gcgatagtgg
    10921 tggtggagaa cgtcgagcgc gtgatgatgg aggataagct cccgccaaaa gaagcgacgg
    10981 aaaaatcaat gtcgcaaatc cagggcgcac tggtgggtat cgcgatggtg ctgtcagcgg
    11041 tatttattcc gatggcattc ttcggcggtt ctactggggc aatttatcgc cagttctcta
    11101 tcaccatcgt ttcggcaatg gcgctttctg ttctggtggc attgattctt acccctgcgt
    11161 tatgtgcaac gctgcttaaa cccgtctctg ctgagcatca cgaaaataag ggcggtttct
    11221 tcggctggtt taatactacc ttcgatcata gcgttaacca ctacaccaac agcgtcggca
    11281 aaatcctcgg ttccacagga cgatatttac tgatctatgc gctgattgtt gcaggaatgg
    11341 tggtgttgtt tttacgtctt ccgtcttcct tcttacctga agaggatcag ggtgtctttc
    11401 tgaccatgat tcagttaccc gctggcgcga cgcaagagcg gacgcaaaaa gtgttggatc
    11461 aagtgacgga ttactatctg aagaacgaga aagcgaacgt tgaaagtgtc tttacggtta
    11521 acggctttag cttcagcggc caggcacaaa acgccggtat ggccttcgtc agtctgaaac
    11581 cgtgggaaga gcgtaatggt gatgaaaaca gtgcggaagc ggtaatccat cgtgccaaaa
    11641 tggaatttgg caagatccgc gacggttttg tcattccatt caatatgcca gccattgttg
    11701 aactgggcac ggcaacgggt ttcgactttg agttaattga tcaggctggg ctgggtcacg
    11761 atgccctaac ccaggcccgt aaccagttgc ttggtatggc ggcgcaacat cctgccagct
    11821 tagtcagcgt gcgtcctaat ggcctggaag acaccgcgca gtttaaactg gaagttgacc
    11881 aggaaaaggc gcaggcatta ggtgtttcac tttctgacat caatcagacc atttcaacgg
    11941 cgttgggtgg gacttacgtt aacgacttca tcgaccgtgg tcgcgtgaaa aaggtgtatg
    12001 ttcaggcgga tgccaaattc cgtatgctgc cagaagatgt cgataaactt tatgtccgca
    12061 gcgccaacgg cgaaatggtg ccattctcgg cctttaccac ttcacattgg gtgtatggct
    12121 ctccgcgact ggaacgctac aacggtctgc cgtcaatgga gattcagggg gaagccgcgc
    12181 caggaaccag ttccggcgat gccatggcgt tgatggaaaa ccttgcgtca aaattacctg
    12241 ctggcattgg ttatgactgg acgggtatgt cgtatcagga acgcttatcg ggaaaccagg
    12301 ctcccgctct ggtagcaatt tcctttgtgg ttgttttcct gtgccttgct gcactctatg
    12361 aaagctggtc aattcctgtc tcggttatgt tggtcgtgcc gttagggatt gtcggcgtgc
    12421 tgctggcggc gacactcttt aatcaaaaaa atgacgtcta ctttatggtg ggcttgctaa
    12481 cgacaattgg cttgtcggcc aaaaacgcta ttttgattgt tgagttcgct aaagatctca
    12541 tggagaaaga gggtaaaggt gttgttgaag cgacactaat ggcagtacgt atgcgtctgc
    12601 gtcctatcct gatgacctct ctcgccttta ttctcggcgt attaccgcta gctatcagta
    12661 acggtgccgg cagtggcgcg cagaacgcag tgggtatcgg ggtaatgggc ggaatggtct
    12721 ctgcaacgtt gctggcaatc ttcttcgtac cggtgttctt tgtggtgatc cgccgttgct
    12781 ttaaaggata aatcagaaac ataaaggcac tttcgggtgc ctttattatt ttcagtgaaa
    12841 cccataaaaa ttaataagat attcttccac tcactcttta aaaactttct atagttcccg
    12901 ctcccttcac tatttttaca attcacataa tttgtcatta ttcattccgc aatatttata
    12961 acctccggtg ataaaatggc attcagctcg ttaataagag agttaactta ttaagcgtta
    13021 gcgtttatta ctgaggtaac accatgaaaa gattaattcc tgtggcattg ctcaccgcat
    13081 tgctggcggg ctgtgctcac gattcgccct gcgttccggt ttatgacgat cagggtcgtc
    13141 tggttcacac caatacctgt atgaaaggca cgactcagga taactgggaa acggctgggg
    13201 ctattgccgg cggggcagcg gctgttgctg gcctgacgat ggggatcatc gctctgtcga
    13261 aataactgaa cagggcgcgg agtttcgcgc tctgtttaac gatacagtat ctttacataa
    13321 gataaaatat tgctatattc tgctggctaa ttctctgctg atttagcatg ttatatcgcg
    13381 ttatttcgct gctttcgaaa taacgcatca atttaacttt cacacttccc accagaaata
    13441 tactcacgct aacaatcatt attctgctta gtaaaacacg cgtaaatatt ttgcgccaaa
    13501 atgtggcgca tgtttcattt tcgcaccatt gcggggcgct gtttttatta tttcctgtct
    13561 acactcagct tattgcgacg cgaaatcggg cgcaaaagct ggcactactt ttgcttataa
    13621 gaggatggcc acagacaggt aaaagacgtt tcccaaacgt cctataacga taattttcgc
    13681 ctcacaggaa gcattatgaa aaagatgatg atagccacac tggctgccgc cagcgtgctg
    13741 cttgccgttg caaatcaggc gcatgctgga gcgacgcttg atgccgttca gaaaaaaggt
    13801 tttgtgcaat gcgggatcag tgatggatta cctgggttct cttatgccga tgcagacggt
    13861 aagttttcag gtattgatgt tgatgtttgt cgtggtgttg ccgctgctgt atttggtgac
    13921 gacacgaaag tgaaatatac cccactcaca gcaaaagaac gcttcacggc tttacagtca
    13981 ggggaagtgg atttgctctc ccgtaatacg acctggactt catctcgcga cgccgggatg
    14041 ggaatggcat ttactggcgt cacttattac gacggcattg gcttcctgac gcacgataaa
    14101 gcggggctaa aaagcgcgaa agaactggat ggcgctaccg tctgtattca ggcgggtact
    14161 gataccgaac tcaacgttgc cgactatttc aaggcaaaca atatgaagta cacaccggtg
    14221 actttcgatc gctctgacga atcagcgaag gcactggaat ctggtcgctg cgatacgctg
    14281 gcctcggatc agtcgcaact gtatgccctg cgcatcaaat taagcaaccc agctgaatgg
    14341 attgtcttac cggaggttat ctctaaagaa ccgcttgggc cggtagttcg tcgtggcgat
    14401 gatgaatggt tctcgattgt acgctggacg cttttcgcca tgctgaatgc tgaagagatg
    14461 ggcatcaatt cccagaacgt cgatgaaaaa gcggctaatc cagcaacgcc tgatatggca
    14521 catctgctgg gtaaagaagg cgattacggc aaggatctga agttggataa taaatgggcc
    14581 tataacatca tcaaacaggt gggtaactac tcggaaattt tcgagcgtaa tgtaggttca
    14641 gaaagcccgc tgaaaattaa acgtgggcaa aataatctct ggaataacgg cggtattcag
    14701 tacgcgccgc ccgtgcgtta agtcggtgtg aggtaacgag cgtcgctacg gcggcgctct
    14761 cttcagcgtt atgctttccg aggtttctat gtctcatcgc cgctcaaccg ttaaaggctc
    14821 actctccttt gccaacccta cggttcgtgc ctggttattc cagatccttg ccgttgttgc
    14881 tgttgtcggc attgttggtt ggttatttca caacactgta acgaatctca gtaatcgtgg
    14941 cattacttca ggttttgcct ttctggatcg cggcgctggc ttcggtattg tccagcattt
    15001 gatcgattac cagcagggcg acacctacgg acgcgttttt attgtcggct tactcaatac
    15061 gctactggtt tctgcattgt gtattgtgtt cgcttctgtt ttgggcttct ttatcggtct
    15121 ggcgagactt tcggataact ggctgctacg aaagctttcc acaatttata ttgagatctg
    15181 aaccgccccg gaaatcctgg agactaaact ccctgagaaa gaggtaaaca ggatgactaa
    15241 aaatactcgt ttttcccccg aagtccgtca gcgggcgatt cgtatggttc tggaaagtca
    15301 ggatgaatat gactcacagt gggcggcaat ttgttccatt gccccaaaga ttggctgtac
    15361 gccggagacc ctgcgtgtct gggttcgcca gcatgagcgg gataccgggg gcggtgatgg
    15421 tgggctcacc agcgctgaac gtcagcgtct gaaagagctg gaacgtgaaa atcgtgaact
    15481 gcgccgcagt aacgatatcc ttcgccaggc ttccgcttat tttgcgaagg cggagttcga
    15541 ccgcctctgg aaaaaatgat gccactgctg gataagctgc gtgagcagta cggggtcgga
    15601 ccggtatgca gcgaactgca tattgccccg tcaacgtatt accattgtca gcaacagcga
    15661 catcatccgg ataaacgcag tgcccgtgcg cagcacgacg actggctgaa gagagagata
    15721 cagcgcgtat acgatgaaaa tcatcaggtg tacggtgtgc gtaaagtctg gcgtcagttg
    15781 ttacgggaag gaatcagggt ggccagatgt acagtggcac gtctcatggc ggttatggga
    15841 cttgccggtg ttctccgggg taaaaaggtc cgtacgacca tcagccggaa agccgttgcc
    15901 gcaggcgacc gcgtaaaccg tcagttcgtg gcagaacgac ctgaccagct gtgggtggct
    15961 gattttactt acgtcagcac atggcagggc ttcgtctatg tggcgtttat cattgatgtg
    16021 tttgccggat acatcgtggg gtggcgggtc tcatcgtcta tggaaacgac attcgtgctg
    16081 gatgcgctgg agcaggcgtt gtgggcccgt cgtccgtctg gcaccatcca tcacagcgat
    16141 aaaggctctc agtatgtgtc actggcctat acggagcgac taaaagaagc cggattactg
    16201 gcatcaacag ggagtacagg cgactcgtat gacaacgcga tggctgagag catcaatggt
    16261 ctttacaaag cggaggtaat acaccgtaag agctggaaaa accgtgcaga agtggaactg
    16321 gccacactaa cgtgggtgga ctggtataac aatcgacgat tgctgggaag gctg
//
LOCUS       DAZGOB010000111        16343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGOB010000111 DAZGOB010000000
VERSION     DAZGOB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.111"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            543..803
                     /locus_tag="ACHLIF_003751"
     CDS             543..803
                     /locus_tag="ACHLIF_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9607685.1"
                     /translation="MNSISVSTPAPAAPVLPVPAQQERFIRLPEVMHLCGLSRSTIYD
                     LISRDAFPQQISLGGKNVAWVQSEVSAWMAARIAAREQGGHA"
     gene            800..1348
                     /locus_tag="ACHLIF_003752"
     CDS             800..1348
                     /locus_tag="ACHLIF_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9607686.1"
                     /translation="MKLALQSHLLFSGLRPDIFSRYSFPAAAKSAAGRRNPSYLLATP
                     DAPCVFFYVVAQAHPFFGLRCLYLSPFQIMVVRAGQPSGWPVSNKAGIPTPVRVTTHE
                     RRNSGGGIYRYLLEVALMATILAPSHPQFIFVFAAVRRAERKPRISMLRIIASNEHHA
                     RLSLVHEYVLCLAARLPVREVI"
     gene            1348..1572
                     /locus_tag="ACHLIF_003753"
     CDS             1348..1572
                     /locus_tag="ACHLIF_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607687.1"
                     /translation="MNHAAISYDDILRLKHLRNVGEFVTGMAVLQDCYEKPASAQCEQ
                     LVSLIYLMTEQLDGVVQRCQDDLMNMEVVQ"
     gene            1569..1892
                     /locus_tag="ACHLIF_003754"
     CDS             1569..1892
                     /locus_tag="ACHLIF_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFO9607688.1"
                     /translation="MKSRTLSLSLRVALYRRAVACAWLSACYQQNRHLQLTLDEIETA
                     IARELEGFYLRQHGQTKGMEIACALLSDLMEYGPLMSCPALSQLGIAVMDELCVRHIK
                     KPVLH"
     gene            1907..4240
                     /locus_tag="ACHLIF_003755"
     CDS             1907..4240
                     /locus_tag="ACHLIF_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO9607689.1"
                     /translation="MSGMKVSQAVKAARGHWAQILPALGVNILKNRHQPCPVCGGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVTRALNVDISEAADRIHGLTHDLSVVNSEVRTL
                     TADTDSGKDAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSAHKAGGMMFRPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRIWIAEGYAT
                     ALTIHYLTGEAVMVAFSSVNFLSLSSVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ASQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSRYESGAWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPSRHLIGFRNGVLDTRTGLFSPHCKENWLRTLCEVDFTPPVK
                     GETLETHAPAFWRWLDRAAGHKPAKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESSRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPILK"
     gene            5146..5970
                     /locus_tag="ACHLIF_003756"
     CDS             5146..5970
                     /locus_tag="ACHLIF_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFO9607690.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSDKTLLTAIRDYIYGEAWYLVYDLIE
                     FIIERTNSYINLSKHLNSIFKKHGVGYTIINGCITPISNDNEIESVQNAVDNGTDSSR
                     SHFERALQLMTDREQPDYRNSIKESISAIESLCRKITGNDKGTLGACLKAIEEKGYIH
                     SAMKGAFSQLYGYTSDQGGIRHALTEEDVNPTLAEAKFMLVTCSAFSNYLLSKISD"
     gene            complement(6019..6591)
                     /locus_tag="ACHLIF_003757"
     CDS             complement(6019..6591)
                     /locus_tag="ACHLIF_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607691.1"
                     /translation="MVDASAQPHIVVTLEPNPWAAFYFDINIANTGNAPAYNVEVVFD
                     PPLVNAEHREKSEIPFSKVSVLKNGQSLTSNLCKYEQIKDQIYNINISWASKPKSNDR
                     ETNEYVYDMATFEGISYLGARSPLTQIAEQIKGIREDWKPIAQGAKKVKADVYTSSDR
                     NEERTYLQEQHDLAIKRRDEKREKRLESGE"
     gene            6692..7862
                     /locus_tag="ACHLIF_003758"
                     /pseudo
     CDS             6692..7862
                     /locus_tag="ACHLIF_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7945..8202
                     /locus_tag="ACHLIF_003759"
     CDS             7945..8202
                     /locus_tag="ACHLIF_003759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607692.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            complement(8760..9527)
                     /gene="fecE"
                     /locus_tag="ACHLIF_003760"
     CDS             complement(8760..9527)
                     /gene="fecE"
                     /locus_tag="ACHLIF_003760"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO9607693.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(9528..10484)
                     /gene="fecD"
                     /locus_tag="ACHLIF_003761"
     CDS             complement(9528..10484)
                     /gene="fecD"
                     /locus_tag="ACHLIF_003761"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO9607694.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(10481..11479)
                     /gene="fecC"
                     /locus_tag="ACHLIF_003762"
     CDS             complement(10481..11479)
                     /gene="fecC"
                     /locus_tag="ACHLIF_003762"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO9607695.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILASIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(11476..12378)
                     /gene="fecB"
                     /locus_tag="ACHLIF_003763"
     CDS             complement(11476..12378)
                     /gene="fecB"
                     /locus_tag="ACHLIF_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO9607696.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(12423..14747)
                     /gene="fecA"
                     /locus_tag="ACHLIF_003764"
     CDS             complement(12423..14747)
                     /gene="fecA"
                     /locus_tag="ACHLIF_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO9607697.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(14834..15787)
                     /gene="fecR"
                     /locus_tag="ACHLIF_003765"
     CDS             complement(14834..15787)
                     /gene="fecR"
                     /locus_tag="ACHLIF_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO9607698.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(15784..16305)
                     /gene="fecI"
                     /locus_tag="ACHLIF_003766"
     CDS             complement(15784..16305)
                     /gene="fecI"
                     /locus_tag="ACHLIF_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO9607699.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
BASE COUNT         3881 a         4151 c         4345 g         3966 t
ORIGIN      
        1 catattctat gtaaagaata aaagatgtct tatgtctgtt taataattaa acattcaacc
       61 tccctgcact cagttgcatt tcaggaaaat attcataaaa atgaatttgt ctgatgttca
      121 ttaaacacat ccggatacat gcggaagtaa gatggtaatt taatttcttt aatcatcgga
      181 tttattgata catatggtga gctgtttatt tttggccgtt ttggctcttt tattaatcag
      241 gtgttgtctt agtgcttatg atgtaacgcc ctgaatgata tgggctggtt cagatttgag
      301 ctgtgatcct gcctagtgta atagactttt ttattattga tgctccttat tatattccat
      361 aaaggaattg tttgtttttg ttcgtttcat acggttttta gctgttttat tttttgagaa
      421 atcaacattt cacacaaaat aaataaagca tgcgcgagcc gcttatataa acataataag
      481 gagctacctg aatccgcatg gaattgcccg gactgttgta aacatattag agaggtagcc
      541 agatgaatag tatttccgtt tccaccccgg caccagctgc tccggtgctc ccggttccgg
      601 ctcagcagga gcgcttcata cgattacctg aagtgatgca cctttgcggg ctatcccgtt
      661 caaccatcta cgaccttatc agccgcgatg ccttcccgca gcagatatcc ctgggtggaa
      721 aaaatgtggc ctgggtacag tcggaggtca gtgcatggat ggcggcgcga attgctgccc
      781 gcgagcaagg aggccatgcg tgaaacttgc tttgcagtct catcttcttt tttctggctt
      841 gcgtcctgat atcttttcca ggtatagttt tcccgctgcc gcaaaatcgg ctgccgggcg
      901 taggaacccg agttacttat tggcgacacc agacgcgccg tgcgtctttt tttacgtcgt
      961 tgctcaggca cacccatttt tcgggctgcg gtgcttatac cttagcccct ttcagataat
     1021 ggtggtccgg gcggggcagc cttcgggctg gccggtttcc aataaggccg gtattcctac
     1081 ccccgttcgg gtcaccaccc atgagcgtag gaactccggt ggtggcattt accgctactt
     1141 attggaggtt gcccttatgg ctacgatcct cgccccgtca catcctcaat ttatatttgt
     1201 ttttgccgcc gttcgtcgtg cagaacgtaa accacgtatc agtatgctgc gtatcatcgc
     1261 cagcaatgag catcatgcac gtctttcact ggtccatgag tatgtgcttt gtcttgctgc
     1321 tcgcctgcct gtccgggagg tgatctaatg aatcacgccg caatctctta tgacgatatt
     1381 ctccgcctga agcatcttcg taacgtggga gagtttgtca ctggaatggc tgttcttcag
     1441 gattgctacg aaaaacctgc cagtgctcaa tgtgaacaac tggtttccct gatttatctg
     1501 atgacagagc agcttgatgg cgtggtacag cgttgccagg atgacctcat gaacatggag
     1561 gtggtgcaat gaaatcacgc actctttccc tttcacttcg ggtagcgttg tatcgccgcg
     1621 cggtagcctg cgcctggttg agtgcctgct atcagcaaaa ccgccatctg cagctcactc
     1681 tggatgaaat tgaaaccgct attgcccgtg agcttgaagg attttatctg cgacagcacg
     1741 ggcaaacaaa aggcatggaa atcgcctgcg ccctgctttc agacctgatg gaatacggcc
     1801 ctctaatgtc ctgtcccgca ctctcccagc tcggaatagc cgtaatggat gagttatgcg
     1861 tccgtcacat caaaaagccg gttttacact gagggagaac tgcgtcatgt caggaatgaa
     1921 agtcagtcag gccgtgaagg ctgcccgcgg tcactgggct cagattctgc cagcgctggg
     1981 cgtgaatata ttgaaaaatc gccatcagcc ttgcccggtc tgtggcggta aagatcgttt
     2041 tcgcttcgac gatcaggaag gacgcggaac atggttctgc aatcagtgcg gagccgggga
     2101 tggtttagcg cttgtcacca gggcactgaa tgtggatatc agtgaagcag ctgacaggat
     2161 acatggactg acacatgatc tgtctgtagt taattccgag gtcaggacgt taaccgccga
     2221 taccgatagc gggaaagatg cagcggcagc acttgccgcg cgactgctgc aagcctcgcg
     2281 tgaatctgcc ggaaacactt atcttacaca taaaggtttc ccggagcata tttgccatga
     2341 gctgacttca gctcataagg ccggtggtat gatgttccgc cccggtgatt tgatcgtccc
     2401 actctataac gctgacgggg agctggtgaa tattcagctc atcagtggca atggtagcaa
     2461 gtgttttctt aaaggaggtc aggttaagga ggcctatcat ctgatagaag ggggcgggag
     2521 ttctgtaaga aggatttgga ttgcggaagg ttacgccacg gcgcttacca ttcattattt
     2581 gacaggagaa gccgtcatgg tggcattttc gtcggtcaac tttctttctc tgagcagcgt
     2641 tgcccataat aagtatccgg gatatcagtt aattattgca gcggaccgcg atctgaatgg
     2701 ttcaggccag aacagggctg aaactgctgc aaaagcgagt cagtgtgaca ttgtgctgcc
     2761 accggttttt ggtgactgga atgatgcgct tacccaatac ggagaggaat ccacccggaa
     2821 ggcaattctt gaggctttga agccacataa cgccagtcct ttcgacacaa tgagtgaagc
     2881 agaattcaca gcgatgagtg tcagcgaaaa agctcagagg gttcgggagc attacaggga
     2941 tgcactggcg gttgatccga acgggcagct tttatcccgc tacgagtctg gagcgtggaa
     3001 agttatttct cagtcagatt ttgcccgaga tgtcgcagcc cttttccagc gcctcggggc
     3061 accgttttcc tcaggaaaaa ttgcctcact ggtggaaaca ttaaagttaa ttgttccgca
     3121 gcagcaaaat ccgtcacgtc atctgattgg ttttcgtaat ggcgttctcg atacgcgaac
     3181 agggcttttt agtccgcact gtaaagagaa ctggctgcgt accctgtgtg aagtcgattt
     3241 cacgccaccg gtaaaggggg aaacgcttga aactcatgcc ccggcattct ggcgttggct
     3301 ggaccgggcc gccggacaca agccagcaaa acgcgacatt attcttgcag cgttatttat
     3361 ggtgctggcg aaccgctatg actggcagct ctttctggaa gtgactggcc ctggtggaag
     3421 tggcaaaagt attctggctg aaattgcgac catgcttgct ggagaggata acgccacctc
     3481 tgcgaccatt gaaacgctgg agtcctcgcg agaacgtgcg gctctgatag gtttttctct
     3541 tattcgcctg cctgatcagg agaagtggag cggcgacggc gccgggctta aggctataac
     3601 tggtggcgat gcagtatctg tggaccccaa atatcagaat gcttattcaa cacatattcc
     3661 ggcggttatc ttggccgtaa ataataatcc tatgcgcttc actgatcgca gtggtggtgt
     3721 gtcccgcagg agagttattc tgcattttcc tgagcagata gcaccggaag aacgcgatcc
     3781 gcagttgaaa gataaaattg cacgagaact ggctgtgatt gttcgtcagc tcatgcagcg
     3841 tttcagcgat ccgatgagcg ccagaacatt gcttcagtcg cagcagaatt ctgatgaggc
     3901 tctcaccatc aagcgtgatg ctgatccagc atttgatttt tgtggctacc ttgaggcatt
     3961 acctgacacc aacggtatgt ttatggggaa cgccaatatt gtcccacgcc agccacgtac
     4021 atacctttac catgcctacc tcgtatacat ggaggctaat ggctataaaa acaccctgag
     4081 tcttacgatg tttggcaaag ggttgccagt catgctgaag gagtacgggc tacattatga
     4141 gaaacggcga actaatcagg gaatgcaaac taacctcact ttgaaagagg agagtaatgc
     4201 agactggctg cccaagtgtg atgagccaat attaaaataa cgattaacta acccggctga
     4261 tgccgggctt cttattggaa acgcacaaat gtagagatag ctgttcactc ttcacctcgc
     4321 tgttaacacg taagcaattg atatataagt taaaaatggc agggtgaata gtgtgcatgg
     4381 ttttgcgaaa aaaaagtatt ttttgtagag tcaagtcccg gtttggatcc gtttaacgga
     4441 acctaactag attgactatt tctctgatga tttttggata aataatcaac aaaaatcaaa
     4501 atgtgaacag agaatggtgg gggcacaaaa aggggcataa ttgaattaat tataattaaa
     4561 tttcaatttt tacaataggt taaatgtgtg tgcgagtccg gccttcgcac caagttacta
     4621 aattccttac aatacagtta gttagacgat taaatctgtc tcttttgcct gtaagaaatg
     4681 ctctgttatg tcccttgaaa aagccgctgt ggttgatagt ggtaattggg atgttaactc
     4741 atagcggcgt gtcgttccaa gcgtcaaaca cgtactgact caacttgaaa gctatgacgt
     4801 agccaccgtc tctaaacaat cccaaaactt ttagtgcagc gcattttagc ccgatgcccc
     4861 cattctgtcg ccgtcgcgag acgccgcagg ggataaacag gtttatggtg gtcacggtac
     4921 ttgcagacca tatgcaagct ctgccagcgg ttcggcctcg ctccccttgg gtacatggtc
     4981 atcctgataa gcctcactaa ctgacataac cctgatgaag tcgcaagagg ctccgcttgt
     5041 tgtcttacca ccaacggggc tttatggatt ctattgctta atatatgatt gtatcaaact
     5101 tagactgcat aatttaaacg aacataacaa tcaggcaggg gaagaatgat aaagccattt
     5161 tctgtgaggt atggtcatgt tgacgttaga gagcatgtcc agctgaatga tcttaattct
     5221 gatacacgca tggcattgtg gaattgctta tatcttttcc tttggactaa caatcgtcaa
     5281 actgctacag caacaaaatg tgcgcagtct gtgtggatat attatctaaa tcaacctgcg
     5341 gataatattc ctcgttatga aagtggatac aaaagtgata agacgttgct gactgctatc
     5401 cgagattata tatatgggga agcatggtat ttggtatatg atttaattga gttcatcatt
     5461 gagagaacta attcatatat taatctatcg aagcatctca attccatctt taaaaagcat
     5521 ggcgttggtt ataccataat aaatggttgt attactccta tttcaaatga taacgaaatt
     5581 gaaagtgttc agaacgcagt tgataatggc actgattcat cgcgtagcca tttcgaaagg
     5641 gcgttacaac taatgacgga tagagagcag ccagactaca gaaactcgat taaagagtct
     5701 atctctgcaa ttgaatcttt atgcaggaaa atcacgggca atgataaagg aaccttgggg
     5761 gcttgtctta aagctataga ggaaaaagga tatatacact ctgcaatgaa aggcgctttt
     5821 agtcagctct atggttatac aagtgatcag ggtggaatac gtcatgcgct aacagaagaa
     5881 gatgtaaatc ctaccttagc tgaagctaaa tttatgttag tcacttgcag tgcgttcagt
     5941 aattatctgt tgtcaaaaat aagtgattaa ttacaggttt atagttacga gtgggttagt
     6001 tacccactcc ctttaaaatt attcaccaga ctctaatctt ttttctcttt tctcatctct
     6061 cctttttatt gccaaatcgt gttgctcttg cagatacgtg cgttcttcgt ttctatcgct
     6121 tgaagtatat acgtctgctt ttactttttt agctccttgt gcaataggtt tccaatcctc
     6181 tcttatacct ttaatttgtt ctgcaatttg cgtcaatggg cttctcgctc ctagataact
     6241 tattccttca aatgtcgcca tgtcatacac atattcattt gtttctctat cgtttgattt
     6301 aggtttgctt gcccagctta tattaatatt ataaatttga tctttgattt gttcatactt
     6361 acagagattg ctggtaagtg attgcccatt ttttaacacg cttaccttac taaacggaat
     6421 ctcacttttt tctctatgct ccgcatttac tagtggagga tcaaacacaa cctcaacatt
     6481 ataggcaggt gcgttccctg tattagcaat gttaatatca aagtaaaaag cggcccaagg
     6541 gtttggctca agtgttacaa ctatatgggg ttgagctgat gcgtcaacca ttctttttgt
     6601 ctcgtctgct ctgaagtggt gaagtggttt actgaatttg gccacctgaa cagaggtgat
     6661 atgctcacct cagaacaaca caggtgctcc aatgaaaaaa agaaatttta gcgcagagtt
     6721 taaacgcgaa tccgctcaac tggttgttga ccagacatac acggtggcag atgccgccaa
     6781 agctatggat gttggccttt ccacaatgac aagatgggtc aaacaactgc gtgatgagcg
     6841 tcagggcaaa acaccaaaag cctctccgat aacaccagaa caaatcgaaa tacgtaagct
     6901 gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg ctacaaccgc
     6961 gctcttgatg tcagactccc tgaacagttc tcgataatca ggaaactcag agcgcattat
     7021 cctgtggtca cactctgcca tgtgttcggg gttcatcgca gcagctacag atactggaaa
     7081 aaccgtcctg aaaaaccaga cggcagacgg gctgtattac gcagccaggt acttgaactg
     7141 catggcatca gccacggctc tgccggagca agaagcatcg ccacaatggc aacccggaga
     7201 ggctaccaga tggggcactg gcttgctggc agactcatga aagagctggg gctggtcagt
     7261 tgccagcagc cgactcaccg gtataagcgt ggcggtcatg agcacgttgc tatcccgaat
     7321 catcttgagc gacagttcgc cgtaacggaa ccaaatcagg tgtggtgcgg tgatgtgacc
     7381 tatatctgga cgggtaagcg ctgggcgtac ctcgccgttg ttctcgacct gttcgcaaga
     7441 aaaccagtgg gctgggccat gtcgttctcg ccggacagca ggcttaccat gaaagcactg
     7501 gaaatggcat gggaaacccg tggtaagccc gtcggggtga tgttccaggc gatcaaggca
     7561 gtcattatac gagcaggcag ttccggcagt tactgtggcg ataccggatc aggcagagta
     7621 tgagtcggcg tggaaactgc tgggataaca gcccaatgga gcgcttcttc aggagtctga
     7681 agaacgaatg ggtgccagcg acgggctatg taagcttcag cgatgcagct cacgcaataa
     7741 cggactatat cgttggatat tacagcgcac taagaccgca cgaatataat ggtgggttac
     7801 caccaaacga atcagaaaac cgatactgga aaaactctaa cgcggaggcc agttttagtt
     7861 gaccacaaca gactacctga agggagccgc ggtcgcctag cagttgcagt agcaggagat
     7921 catccagccg cagtacaggt cacgatgact ctggttaatg ataccggctt tgaccccgta
     7981 ttttccggct ctatcgctga atcatggcgt cagcagccgt gcacaccatc ctattgttgt
     8041 gactgggagg ctgccaccat gcttcgcgct ttccctctgg cgaaaaaggg agaaggacgg
     8101 gcccgtctgc cttcacttta tgccagcttc ggtaagctgg gtgagacacc gactcataaa
     8161 gatatcattg ataacaatcg atccatcaac tggcctgtat aacgtggctg ccggtgatta
     8221 agaaagctgc acctacctaa gtagtagcaa acgcacactt tttagaaaaa tcgatggtca
     8281 gaaactggat tagcaattcc gttccagggt tgcttttgat ttacgttggc gtctgatcat
     8341 tgatttatcc tcaaaagccc aacctcattg gtaatgaacc agctccgtga atgtccgctc
     8401 tggcacagag cgaaattttt tgatctcccc ccctgaaatc taaacttagt catgtcacgt
     8461 ttttgggttt ctaaaatttt aacttcgcgt ttttcgttgc cgtaagggtt atacagaaat
     8521 gtccgttaag cagagttcaa aattgattgc cgtgatcacg actggtttga aagccgcgcc
     8581 caagcctgta cagctctggt ttgcgttgat tatgaacctg tcagcctaaa gcaagcggat
     8641 ggacgatgag tattggtaat ctttcagagt ccggaaaagt tcagccccag tctgaacagg
     8701 cttgctggcg ccagtccagt ttcattcagt cgtggtttgg ttcttacggc ctgtgcaatc
     8761 tacctcatta ggcacatcgg cctgccagat accggctcgg ggtgtatttc cgcttccacg
     8821 ctgaatactg ttctcagcaa tcctggggtc atcacctctt ctggtgtgcc ttgcgccata
     8881 acatgtccgt ttgccattac caccagttga tcgcagtacc ggctagcctg attaaggtcg
     8941 tgcagcacag cgaccaccgt tttcccctga gtccggagtt cgcccatcaa ccgcatcagg
     9001 tccacctggt gattgatatc aagataggtg gttggctcat caagtaatac aacgggcgta
     9061 ttctgggcca ggaccatcgc cagaaatgcg cgctggcgct gaccgccgga aagctcggtt
     9121 aaccgacgaa cggcaagatg attgatccgg gtctggttca tggcgacatt aactcgtgca
     9181 ttgtcttcag cggagagacg cccccagagt gacagccagg gattacgacc atacgaaacc
     9241 agctcctgga ctgtgatccc ctctggcgtt aaatggtgct gaggcagcag cgaaagcctg
     9301 cgggccaact ggcgcgatga gagcatattt atgggattat cgccgagaaa tacggtgcca
     9361 gactgcggca ttaaaagccg cgaaaaacag tttaacagcg tcgatttccc gcaaccgtta
     9421 ggaccgatca gggcggtgat cttccccgtt ggcagtgaga gtgaaacgtc gttaagtacc
     9481 ttgtctgtcc cgtaactgac cgtcagattt tcagttcgta aagtcattta tcgcattctc
     9541 acaagcaacc agacaaacca cggcgcaccg ataatggcgg tcagcacgcc aaccgggagc
     9601 tccagtgggg gatgaataat tctcgccagc agatcggcaa ccaccaacag caacgcacct
     9661 gtcagggctg aaacaggcag cagtctgcgg tgacgtccac cggtgatgct acgcatcata
     9721 tgcggcacca cgagaccaat aaagctaatc gggccgcagg cggccacgcc ggtagatgtc
     9781 atggcgacag ctagtaacaa agcccagaat cgggtatggg gcaccgacac accgagcgtg
     9841 gtggcgcgcg catcgccgag tgcaaggagg tcgagatcgc ggcaaaaact caggctcagc
     9901 ggcagaaata aaatcatcag cgggatggca atcttcacaa agctccagtc acggccccat
     9961 aagctgccgg tcagccacag cagggcgttg ttcacatcct gtgggcgcga gagcatcaga
    10021 taatccgtca ggctggccca gcatgcagaa agcgccacgc cggtgagcgc cagcttcatc
    10081 ggctggtggg tctttgccag catcttcagt aatatcaacc ccgccatgcc gcccgcaaag
    10141 gccagcagcg gcagcaccat cacgggcagt gacggcataa gaagtagagc ccccacagag
    10201 gccaggctgg cggcatggtt aacgccgaga atatccggtg atgccagagg gttgcgcaca
    10261 atcccctgta tcagcacgcc cgccacggcg agggctgcac cgacaaacag tgccagcagc
    10321 aagcgcggca gtcggtactc catcaataca taataatgct cgcgtccggc ctgccagtcg
    10381 gtcagcagcg cgcgccacgg cacggggatc actcccatat ggagtgataa cagcgcacag
    10441 cccgccaggg caagggtgat gaaaataacc agcgcaattt tcatcctcgc ctcctcacaa
    10501 gccagacaaa gcaagggctg ccaatcagcg ccagcactgc gcctgcgggc agatctccgg
    10561 ggaaggccag cgcgcgtgcg agtacatctg ccagcagcat cagcgtggcc cccagcagca
    10621 tgctcactgg cagtacgttg cgctgatcga agcctgccca gaagcgcgcc agatgtggca
    10681 ccagcagacc gataaacgcc accggacctg ccacgctgac gcacgcacca accagaagca
    10741 gcactaacat attgatgacc aaacgtagcc tcgtcaggtt cactcccagc gtatgggcgg
    10801 tgctgtcgct gaggttgagc aggttcagtt gattcgccag cagcaacacg acagggactg
    10861 cagtgaccac caccggcaag agctgccaga catcctgcca gcgggcgtgg gacactccgc
    10921 ctgccagcca gtaaaagatg ccgtaagcat gatcttcggc cagcagcagg gtgatgcggg
    10981 taaggcccat acaaaaggcc gacagcgcga tactcgcgag gatcagtttg tttctgtcat
    11041 gggtatgacg aaatccgcct cctgcggtca tgaccagcag ccagctcacg ccgcccccgc
    11101 atgccgcgat gaacgacaga gaatagcctg caatcggcgt cggactcagc gcgctggtaa
    11161 gcgccatagc cagcgccgcg ccgctgttaa tgccgagcag tgaaggagag gccattgggt
    11221 tgtgggtcag ggtttgcagc agcgtgcccg cgagcgccag gcttgcgccg atcagaacgg
    11281 cgaccaggct tcgtggcaaa cgaaggtttt gcaccagcgc ttctggtagc gttggcgtgt
    11341 gtccaggcag cagggcgcgg gttgcatctg ctccggaaac aggaatggcc gagtagcaaa
    11401 acagactcag ccagaaaata ataataagtg ctgcaacggg aagcccccac agcagcaccg
    11461 ggtgttttat cgcggtcatt tcacaacggt aagcggctga tggtggaaga tttttaccgt
    11521 gtcagcggca atacgctctg cagcaaaaat accgcgcatc cgcgcccagg tgttactgtc
    11581 gaccgaagca acctgctgct tctgcgcggc ggttaacatc tgccagagcg gatcttgttg
    11641 ccagcgttta acaatgctct cttcgcgata gtgggcaacc agcagccagg caggattgac
    11701 cgccagcagt tgctccaggc cgatggacgg catggacgca cccgccatcg cagcgggaac
    11761 gttcagcccc agagaggcca gcacgctgcc ggtccaggtc tcctgagtat gcaggttgaa
    11821 ttgctgttcg cgtgatgtgc caaaggccac gcgtgtccct ttgggaagct ggctggccca
    11881 ctgcgccatc ctctctttat gttgttccag gcgtgcctgc atctctcgct ttttacccac
    11941 catttcgccg atgatagccg cagattgcaa attttcagcg taggtttcgt tgcgggactt
    12001 aagcagcagt accggcgcga tttgctgcaa ggcgatgtaa acccccgcat ggcgactgct
    12061 gtcggcaatg atcaggtctg gtttcagagc ggcaatggct tccaggctcg gctgcgcgcg
    12121 cgttccgacg gactgccacg gtttcaggtg cgcacgcact tcgggcagga tgcgttttgc
    12181 atcgttatcg tcggcaatac cgatcgggct gacgtccacg gcggccagcg catcggcgaa
    12241 cgagagttcc agcaccacaa tccgttgtgg cgttttttcg agtgtaaacg tgccgtgttc
    12301 gtcctgaacc gtggcggcaa aggcgtggct gatcaccagc agcaggcctg caaaaacaaa
    12361 acggataaat gccaacataa tcacattcca gctaaaagcc cggcaagccg ggcgttaaca
    12421 catcagaact tcaacgaccc ctgcatatac agcgtgcgcg gctggcctgc atagatgcct
    12481 ttgttgttgt cgtcataaga gcggatgaag tagtcctggt cgaagatgtt tttcacaccg
    12541 aatgccaggt tcagatctgc catctgcggg ccaaaatcat acgccacgcg tgcgccccag
    12601 agcatgaagc cgggaatgcg gccggtactg ccgtcggcgc tctctttcac cgtattggcg
    12661 ttatccgcaa actggctgga ctggaaatcg ctgttcagat tgaacgtcca gtttcctggc
    12721 ttgtagtcca cgcccagcgt gcctttatgt ttcggggaga atggtaccag attgccgtag
    12781 gtgtcgcctt tctcgcggat ttccgcgttc acatacgcat agctggcgta gatggaaacg
    12841 ttatcaagcg ttggcgttag cgtacccaga tcgtaacgtg cctgcgtttc cagcccggta
    12901 tggcgcgttt tgccacgtgc agtgacggtg tcgttggtct ggttggagtc gtactgattg
    12961 ttaaagttaa tcaggaacag ccccatttcc gccgtcagcg cgccgtcgtc gtagcgggta
    13021 ccgagttccc aggttcgcgc tttttccggt tccacattgc cgctttgcac agccttgcca
    13081 atctggctgt actgtacggt gccgaacgag ccttcagtgt ttgcataaag attccagctg
    13141 tcagtcaggt gatagagcac gttcaacgcc ggaagcggtg cgttatagct cacttcttcg
    13201 tgcgtgcctg tgatggcgtt gttctggtat gactcgatat gttcgaaacg catacccggc
    13261 gtgatggtcc agttgccgat gtcgattttg tcatccagat accaggcgtg cgcctcggtg
    13321 ccggaacgcg tatcgcggtc gtaagggctt gagccggacg gcaactgccc gctgctggtg
    13381 gcggtgtagt aacgcatttc atgcgttgat tcattcaaat agcgatagcc cacgcccact
    13441 tcgtgcgcgg aagggccgat cataaagatc tggctgtagc gtggctcaat accgcgcacc
    13501 cagtagttac gcggcgagag ggtgatgcgt ttgccttgct ccaggtagcc gctgcgcagg
    13561 gtttgggtgt agaacccctg aatgttgaat ttatgctggc tgtctggctg gaactggtag
    13621 cccaggctcg ccagcttgcg acgaccccag aagcggtcat acgggcgggt ggattgccag
    13681 cgatcggcgt cgtaatccgc gcgagacagg ccaccgggca tgtcggcttc accatcgtaa
    13741 tattgcagca ggctgttgaa ggtgtgcacc tcatccggcg catatttgct tttcagcatc
    13801 aggtcgtcga tgcgggtggc gctgtgctcg cgccagtcac tgccgcgcgt gccggagtag
    13861 agcagcgcgg tgccaaaacc gttgtccgct gtgccgccca ccatcaggtt gtgcgtctct
    13921 ttcgggttgt tttgtgaaga ggttgggctg agctgacctt ccacgcccgc ctcgatacca
    13981 aagtcctgcg gaatggcacg ggtaacaaag ttcaccacgc cgcccacgct ctgcggtccg
    14041 taacgcaccg caccaccacc gcgtaccacg tcaatggcat ccatgttgcc gagcgaaacg
    14101 ggagccagtg aaagctgcgg ctgaccgtaa ggggcgaagg ggacggggat gccgtccatc
    14161 aggacggtcg agcggctggc gaggcgcggg ctcaggcccc ggatgccaaa gttcatcgcc
    14221 aggtcgtggc tgccggtgcc gttgttttcc ggcgcgctga cgccagggat gcggttaagt
    14281 acctcacgca tggtggttgc gccggttttg gcgaaatcct cacggcggat cacgtcacgc
    14341 gcgccagcat gttcaaatac gtcgttttca cgcgcatcac ccagccagtc gccgaccacg
    14401 gtcagggcat cttcttttgg tgcgggcgcg ggctccagcg tccagctgtt atttcccagc
    14461 ggttttacct gcagtccgct gccgtccagc agttgttgca ggccgctctc gacgtcgtaa
    14521 tcgccgtgca ggccgttgct ctgcttgccg cgcgtcaggc tggcgtcaac cgagagggta
    14581 aatccgctgt gtgcggcata ctgattgagc gctttatcga gcgatcccgg tgcgatatta
    14641 acctgtgcag caaaagcgga aaacgagaga ccggccagcg gcagcaggct caggcgaatg
    14701 gcgttaacca aaggtgttgt tttacgaaaa acgcgtaacg gcgtcatacc ttccccatca
    14761 tcatttttgt tgtgttcagc tatgagtcga acgagaaggg taaaaaggac aatcgaaata
    14821 agaattattt tccttacagt ggtgaaatgt ttatccagta ccgcgtaata gactgaattt
    14881 taacgggaag cgtttgcgcg ataacgttca ggatcgcatc ggtatttttc agcgggaacg
    14941 tcccgctcag gcgcagcccg gcaacggcgg gatcgcagcg cagcacgccg ttgcggtaac
    15001 gggttagcgt ggctatcacc tcacccagcg gtttatcgct gaagctcagg atgtccttcg
    15061 tccagcttgt actctcgtca tccagcggtt tcactgcgcc aaactcagag gcgctgaact
    15121 gcaggctttc accagcgttc acgatgcgtt tttgcgcggg ggcactggcg agaagcactt
    15181 ccacagcgtg ctgctgcacg tcaagctgcg tgaaattatc ctgctggcgg acggtaaatt
    15241 ctgtccctaa agcagtgagc tggccctgac gggtcaggac acggaagggg cgttgcaggg
    15301 catctttcgc ggtagtaatg gcgatttcac cgtaccagag ccggacggtg cgctgatgcg
    15361 catcaaaacg cacatccgcc gcgctttggg tattcagcgt gagcagggag ccatcttcca
    15421 gttgctgacg gctgactgtg cctttggcgg tgcggtaatc tgcccgcaga ccttcgccgg
    15481 tttccgactg ccagagctgc cagcctccgc cagcgccgag caacagcagt aatcctttca
    15541 tcacgtgacg gcgggtgagg cgggtatcgt gcaacgccct gctggctacg tcgccaggca
    15601 caccgccaag ctggttgcgc aggttttcaa cctgctgcca ggcccactgg ttatcctgat
    15661 cctgttcata ccactgttgc cagcgcgctt cctgttgtgg gctgacgcgc tcgccgctta
    15721 gcacggcata ccagtgggaa gctgaacgca gcgcctgacg gcgggaatcg gttaacaaag
    15781 gattcataac ccatactcca gacggaacag caggcagtgc tcgacggctt tcgccacgta
    15841 ttttttcacg gagctgatgg aaacaccgag tttgtgcgca atctcgctgt atgtcagacc
    15901 atccagttgc gaaagcagaa acgcttcacg tgttttgccg tttagcccgt ccagcatgct
    15961 gtcgaggagt tgtagggtct cgagttggct ttcgcgttcc tcaggtgaag gcgctccccc
    16021 ctccggcata agcgccagca tctccagata cgctttttcc agcgcgtttc ggcgaaacag
    16081 gtccaccatc acgcgtttgg cgatagtgca gaggaaggag cgaggatcgc ggatcgtcga
    16141 gagcgtttcg ctgaccatta cccgcaaaaa agtgtcctgg gcaatgtcat ctgcatcaaa
    16201 agcagactgg agtttgcgcg tcagccagct tttcaaccag ccgtgatgtg tgccataaag
    16261 cgactcgaac gttaaggaag ctgtggtagt ggcgcggtca gacatgcgga gtgcatcaaa
    16321 agttaattat cacgtagtca tat
//
LOCUS       DAZGOB010000112        16094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGOB010000112 DAZGOB010000000
VERSION     DAZGOB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.112"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..150)
                     /gene="yojO"
                     /locus_tag="ACHLIF_003767"
     CDS             complement(<1..150)
                     /gene="yojO"
                     /locus_tag="ACHLIF_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO9607700.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCS"
     gene            259..753
                     /gene="napF"
                     /locus_tag="ACHLIF_003768"
     CDS             259..753
                     /gene="napF"
                     /locus_tag="ACHLIF_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO9607701.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            743..1006
                     /gene="napD"
                     /locus_tag="ACHLIF_003769"
     CDS             743..1006
                     /gene="napD"
                     /locus_tag="ACHLIF_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO9607702.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1003..3489
                     /gene="napA"
                     /locus_tag="ACHLIF_003770"
     CDS             1003..3489
                     /gene="napA"
                     /locus_tag="ACHLIF_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HFO9607703.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3496..4191
                     /gene="napG"
                     /locus_tag="ACHLIF_003771"
     CDS             3496..4191
                     /gene="napG"
                     /locus_tag="ACHLIF_003771"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO9607704.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4178..5041
                     /gene="napH"
                     /locus_tag="ACHLIF_003772"
     CDS             4178..5041
                     /gene="napH"
                     /locus_tag="ACHLIF_003772"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO9607705.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGYICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5038..5487
                     /gene="napB"
                     /locus_tag="ACHLIF_003773"
     CDS             5038..5487
                     /gene="napB"
                     /locus_tag="ACHLIF_003773"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO9607706.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5497..6099
                     /gene="napC"
                     /locus_tag="ACHLIF_003774"
     CDS             5497..6099
                     /gene="napC"
                     /locus_tag="ACHLIF_003774"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO9607707.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6112..6735
                     /gene="ccmA"
                     /locus_tag="ACHLIF_003775"
     CDS             6112..6735
                     /gene="ccmA"
                     /locus_tag="ACHLIF_003775"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO9607708.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6732..7394
                     /gene="ccmB"
                     /locus_tag="ACHLIF_003776"
     CDS             6732..7394
                     /gene="ccmB"
                     /locus_tag="ACHLIF_003776"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO9607709.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VSGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7436..8173
                     /gene="ccmC"
                     /locus_tag="ACHLIF_003777"
     CDS             7436..8173
                     /gene="ccmC"
                     /locus_tag="ACHLIF_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO9607710.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8170..8379
                     /gene="ccmD"
                     /locus_tag="ACHLIF_003778"
     CDS             8170..8379
                     /gene="ccmD"
                     /locus_tag="ACHLIF_003778"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO9607711.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8376..8855
                     /gene="ccmE"
                     /locus_tag="ACHLIF_003779"
     CDS             8376..8855
                     /gene="ccmE"
                     /locus_tag="ACHLIF_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO9607712.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8852..10795
                     /gene="ccmF"
                     /locus_tag="ACHLIF_003780"
     CDS             8852..10795
                     /gene="ccmF"
                     /locus_tag="ACHLIF_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO9607713.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10792..11349
                     /gene="dsbE"
                     /locus_tag="ACHLIF_003781"
     CDS             10792..11349
                     /gene="dsbE"
                     /locus_tag="ACHLIF_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO9607714.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11346..12398
                     /gene="ccmH"
                     /locus_tag="ACHLIF_003782"
     CDS             11346..12398
                     /gene="ccmH"
                     /locus_tag="ACHLIF_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO9607715.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQENK"
     gene            complement(12433..13080)
                     /gene="narP"
                     /locus_tag="ACHLIF_003783"
     CDS             complement(12433..13080)
                     /gene="narP"
                     /locus_tag="ACHLIF_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO9607716.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13400..15991
                     /gene="yejO"
                     /locus_tag="ACHLIF_003784"
                     /pseudo
     CDS             13400..15991
                     /gene="yejO"
                     /locus_tag="ACHLIF_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         3586 a         3949 c         4683 g         3876 t
ORIGIN      
        1 ggagcaaacc agcagaaata tctctggtga attaatcgct atataaatat gtttataacc
       61 atttgaaatg tgagcaaaag cccgtttttg ccacgcttcc cgccactctt ttgatcctgc
      121 tacaggtttt accccgatcg gggtatgcat ctttgacaca tcctttaata tcttagcggc
      181 tataaaaatg gcttattaat tatgcggttt atttggtcgc tctcaatttt cagagcgcgt
      241 taatgatgga aggtcaatgt gaagattgat gcatcccgtc ggggcatact cactggtcgc
      301 tggcgcaaag ccagtaacgg tatccgtccg ccctggtccg gtgatgaatc tcattttctg
      361 acccattgca cccgctgcga cgcctgtatt aatgcttgtg aaaacaacat tctgcaacgc
      421 ggcgcgggcg gctatccgag cgttaatttc aaaaataacg agtgcagctt ctgttacgcc
      481 tgcgcgcagg cttgccccga atcgctattt tctccgcgcc acaccagggc ctgggatttg
      541 cagttcacca ttggggacgc gtgcctggcg tatcagtcag ttgaatgccg ccgctgtcag
      601 gatagctgtg agccaatggc aattatcttt cgcccgacgc tctccgggat ctaccagccg
      661 caacttaata gccaactttg caacggatgt ggagcatgtg cagccagctg tccggtatca
      721 gccataactg cggagtatct ccatgcacac taactggcaa gtttgcagcc tggtcgtgca
      781 ggccaaaagc gaacgaattt cagacatcag cacccaactg aacgcctttc ccggctgtga
      841 agttgctgtc agcgacgcgc cgagcggtca gttgattgtg gtggtggaag cagaagacag
      901 cgaaacgctg atccaaacca ttgagtcagt acgcaacgta gagggcgtgc tggcggtgtc
      961 gctggtttat caccagcagg aagagcaagg tgaggaaaca ccatgaaact cagtcgtcgt
     1021 agctttatga aagctaacgc cgttgcggcc gctgcggcgg ctgccggtct cagcgtgccg
     1081 ggcgttgccc gcgccgttgt tggtcagcag gaagccatta aatgggataa agcgccgtgc
     1141 cgtttctgcg gtactggttg cggcgttctg gtcggaacgc agcaggggcg tgtggtggcc
     1201 tgtcagggcg acccggacgc accggttaac cgtggcctga actgcattaa gggctatttc
     1261 ctgcccaaga tcatgtacgg taaagaccgt ttgacgcagc cgctgctgcg tatgaaaaac
     1321 ggtaaatatg acaaagaagg cgaatttacc ccaatcacct gggatcaggc cttcgatgtg
     1381 atggaagaga aattcaaaac cgccctgaaa gaaaaaggac cggaatcgat cggtatgttc
     1441 ggttctggtc agtggactat ctgggaaggt tatgccgcgt ccaagctgtt caaagcgggc
     1501 ttccgttcga acaacatcga cccgaacgca cgtcactgta tggcgtcggc agtagttggc
     1561 tttatgcgta cctttggtat ggatgagccg atgggctgct atgacgacat cgagcaggct
     1621 gacgcgtttg tgctgtgggg cgctaacatg gcggagatgc acccgatcct ctggtcacgc
     1681 atcactaacc gtcgtctctc taaccagaac gtcaccgtgg cggtgctttc tacctaccag
     1741 catcgtagct tcgagctggc ggataacggc atcatcttta cgccacagtc tgacctggtg
     1801 atcctgaact acatcgccaa ctatatcatt caaaacaatg cgataaatca ggacttcttc
     1861 agcaagcacg ttaacctgcg caaaggggcg acggacatcg gctacggttt acgtccgacc
     1921 catccgctgg aaaaagcagc gaagaatccg ggttctgacg cctccgaacc gatgagcttt
     1981 gaagattaca aagccttcgt tgccgagtat acgctggaaa aaactgccga aatgaccggc
     2041 gtgccgaaag accagttaga acaactggcg cagctgtatg ccgatccgaa caagaaagtc
     2101 atctcctact ggacgatggg cttcaaccag catactcgtg gcgtgtgggc caacaacctg
     2161 gtctacaacc tgcacctgct gaccggcaaa atttcccagc cgggttgcgg tccgttctcc
     2221 ctgaccgggc agccttccgc gtgtggtact gcgcgtgaag tgggcacctt tgctcaccgt
     2281 ctgcctgcgg acatggtggt gactaacgag aaacaccgtg atatttgcga gaagaagtgg
     2341 aatatcccga gcggcaccat tccggcgaaa atcggtctgc acgcggtggc acaagaccgt
     2401 gcactgaaag acggcaagct gaatgtttac tggaccatgt gtaccaacaa catgcaggcc
     2461 gggccgaaca ttaatgaaga gcgtatgccg ggctggcgcg atccgcgcaa cttcatcatc
     2521 gtctccgatc cgtatccgac agtcagtgcg ctggcagccg acttgatcct gccgaccgca
     2581 atgtgggtag agaaagaggg cgcttacggt aacgccgaac gccgtactca gttctggcgt
     2641 cagcaggtac aggcgccagg cgaagcgaaa tcggatctct ggcagttagt ccagttctcc
     2701 cgccgcttca aaactgaaga agtatggccg gaagagctgc tggcgaagaa accggaactg
     2761 cgtggcaaaa cgctgtacga agttctgtat gccacccccg aagtgagcaa ataccctgta
     2821 tccgaactgg cggaagatca gctgaacgat gaatcccgcg agctgggctt ctatctgcaa
     2881 aaagggctgt tcgaagagta cgcatggttt ggtcgcggtc acggtcacga tctggcaccg
     2941 ttcgatgact accacaaagc gcgcggtctg cgctggccgg tggtgaacgg taaagaaacg
     3001 cagtggcgtt acagcgaagg taacgacccg tacgtgaaag cgggcgaagg ctataagttc
     3061 tacggtaaac cggatggcaa agcggtgatc ttcgcactgc cgttcgaacc ggcggcggaa
     3121 gcaccggatg aagagtacga cctgtggctc tctaccggac gcgttctgga gcactggcac
     3181 accggcagta tgactcgccg tgtgccggaa ctgcaccgcg ccttcccgga agcggtcttg
     3241 tttattcacc cgctggatgc gaaagcgcgc gatctgcgcc gtggcgacaa agtgaaagtg
     3301 gtttctcgcc gtggcgaagt gatctcgatt gttgaaacgc gcggtcgtaa ccgtccgcca
     3361 cagggcctgg tgtacatgcc gttcttcgac gccgcacagc tggttaacaa actgacgctg
     3421 gatgcgaccg atccgctctc gaaagagacg gacttcaaga agtgcgcagt caaactggag
     3481 aaggtgtaag ccattatgtc ccggtcagcg aaacctcaaa atggtcgccg ccgctttctg
     3541 cgcgatgtcg ttcgcacagc aggcgggctg gctgccgtgg gtgtggcgct ggggttacaa
     3601 cagcaaaccg cacgcgcatc tggcgtgcgg ttgcgcccgc ccggagccat aaacgagaac
     3661 gcctttgcca gtgcctgtgt gcgttgcggt cagtgtgttc aggcttgccc ttacgacacc
     3721 ttaaaactgg cgacgctggc ctctggtctg tcggcgggca cgccatattt tgtcgcacgg
     3781 gatattcctt gcgaaatgtg tgaggacatt ccgtgcgcca aagtgtgccc aagcggtgcg
     3841 ctggatcgtg agattgaatc gatcgacgac gcgcggatgg ggctggcggt actggtggac
     3901 caggaaaact gtctcaactt tcaggggctg cgctgcgatg tttgttatcg cgaatgcccg
     3961 aaaattgatg aggccatcac cctggagctg gagcgcaaca cgcgtaccgg taagcacgcc
     4021 cgctttctgc cgacggttca cagcgacgcc tgtaccggtt gcggtaagtg cgaaaaagta
     4081 tgcgtgctgg aacaaccggc aatcaaggtg ttaccgctgt cactggcgaa aggggagtta
     4141 ggtcaccatt accgcttcgg ttggctggag gggaacaatg gcaaatcgta aacgtgacgc
     4201 cgggcgcgag gcgctggaga aaaaaggctg gtggcgcagt caccgttggc tggtgttacg
     4261 tcgcctttgt cagttcttcg tgctggggat gtttttgagc ggtccatggt ttggtgtgtg
     4321 gatcttgcac ggcaactaca gcagtagcct gttattcgac accgttccgc tgaccgatcc
     4381 gctgatgacg ctgcaaagtc ttgccagtgg tcatctgcct gccacggtgg cgttgaccgg
     4441 tgcggtgatt atcaccgtgc tctacgccct ggcgggaaag cgattatttt gcagctgggt
     4501 ctgcccgctg aacccgatta ccgacttagc gaactggtta cgcaggcgct ttgacctcaa
     4561 tcagtctgcg acgatccctc gccacattcg ctacgtgctg ttggtggtca ttctgctggg
     4621 atcggcctta actggcacgc tcatctggga atggatcaac cccgtatctc tgatggggcg
     4681 cagcctggta atgggcttcg gcagcggcgc gctgcttatt ctcgcgctgt ttttatttga
     4741 tctactggtc gttgaacacg gctggtgcgg gtacatttgt ccggttggcg cgctgtatgg
     4801 cgtgctgggt agcaaaggtg tgattaccgt tgcggccacc gatcggcaga aatgtaaccg
     4861 ctgtatggat tgttttcatg tttgcccgga accgcatgtg ctacgtgccc cggtgctgga
     4921 tgaacaaagc ccggtgcagg tcaccagccg cgattgcatg acgtgcggtc gctgcgtgga
     4981 tgtctgttct gaggatgtat ttacaataac tacacgatgg agttcgggag cgaaatcatg
     5041 aaaagccatg acctgaagaa agcgctgtgt caatggacgg cgatgctggc cctggtggta
     5101 agcggcgcgg tttgggcggc taatggcgtc gattttagcc agtcgccgga agtatccggg
     5161 acacaggaag gggccattcg gatgccaaaa gagcaggaac ggatgccgct gaactatgtg
     5221 aatcagccgc caatgatccc gcatagcgtt gaaggttatc aggtaacgac caataccaac
     5281 cgctgcttgc agtgccacgg tgtcgaaagt tatcgcacca ctggcgcgcc gcgtatcagt
     5341 cctactcact ttatggacag cgacggcaaa gtgggcgcgg aagtggctcc gcgtcgttat
     5401 ttctgtctgc aatgtcacgt accgcaggcc gataccgcgc caatcgtggg gaataccttt
     5461 accccatcaa aaggttacgg gaaataagag gttattatgg gaaattctga ccgtaagcct
     5521 ggtctgatta agcgcctgtg gaaatggtgg cgtaccccca gccgtctggc gctggggacg
     5581 ctgctgttga tcggttttgt tggcggcatc gtcttctggg gtggctttaa caccgggatg
     5641 gaaaaagcca ataccgaaga gttctgcatt agctgccacg aaatgcgcaa cacggtgtat
     5701 caggaataca tggattccgt gcactacaac aaccgtagcg gcgtccgtgc gacctgtccg
     5761 gattgtcacg ttccgcacga gtttgtgccg aagatgatac gcaagctcaa agcaagtaaa
     5821 gagctgtatg gtaaaatttt tggcgttatt gacacgccgc agaaatttga agctcatcgt
     5881 ctgacgatgg cacagaatga gtggcggcgc atgaaggaca ataactcgca ggagtgccgt
     5941 aactgtcaca acttcgagta tatggataca accgcccaga aatcggttgc cgcgaagatg
     6001 catgaccagg cggtgaaaga tgggcaaacc tgtattgatt gccataaagg gatagcgcac
     6061 aagctgcccg atatgcgtga agtcgagcca ggtttttaac agggttattg cgtgggtatg
     6121 cttgaagcca gagagttact ttgtgagcgg gatgaacgaa ccttatttag tggcttgtca
     6181 tttacgctga acgcaggaga gtgggtacaa atcaccggta gcaacggcgc ggggaagaca
     6241 acgcttctcc gtttgctgac ggggttgtct cgccctgacg caggcgaggt tctctggcaa
     6301 gggcagccct tgcatcaggt acgcgacagc taccatcaaa acctgttatg gataggccat
     6361 cagccgggga tcaaaacccg gctgacggcg ttagaaaatc tgcactttta tcatcgcgat
     6421 ggcgataccg cacaatgtct ggaagccctg gcgcaggccg ggcttgccgg attcgaagat
     6481 attcctgtaa atcagctctc ggccgggcaa caacgccgcg tcgctttagc gcgtctgtgg
     6541 ctgacccgtg ccacgttatg gatcctcgac gagcctttta ccgcgattga cgttaacggc
     6601 gtcgatcgtc tgacccagcg tatggcgcag catacggagc agggggggat tgtgattctg
     6661 actacccacc agccgctcaa cgttgctgaa agtaaaattc gccgcatttc actgacgcaa
     6721 acgagggccg catgatgttc tggcgcattt tccgtcttga gctgcgtgta gcgtttcgcc
     6781 atagcgccga aatcgccaac ccgctgtggt tcttcctgat tgtaattacc ctttttccgc
     6841 tcagtatcgg tccggagccg caactgctgg cgcgtattgc accgggcatt atctgggttg
     6901 ctgcactgct ttcatccttg ctggcgctgg aacgactgtt ccgtgacgat ttgcaggacg
     6961 gcagtcttga acaattgatg ttgttgccgt tacccttgcc cgccgttgtg ctggcgaagg
     7021 tgatggcgca ctggatggta agcggtctgc cgttactcat tctttcgcca ctggtagcaa
     7081 tgctactggg aatggatgtt tatggctggc aagtgatggc gctgacgctg ctgctgggaa
     7141 cgcctacgct tggctttctc ggtgcaccgg gcgtggcgct gacagtggga cttaagcgcg
     7201 gtggtgtgct gctcagcata ctggtgttac cgctgactat cccattactc atcttcgcca
     7261 ccgccgcgat ggacgcggct tccatgcatt tgcccgttga cgggtatctg gcaattttag
     7321 gcgcgttgct ggcaggcacc gcgacattaa gtccttttgc gacggcggca gcgttacgaa
     7381 tcagcattca ataacgctgg attactgatt tttgttcgag tctggtatcg aaactatgtg
     7441 gaaaacactg catcaactgg cgatcccacc acggctgtat caaatctgtg gctggtttat
     7501 accgtggctg gcaattgcca gtgtggtcgt gcttaccgtc ggctggatct ggggattcgg
     7561 ctttgctccg gctgattatc agcagggaaa tagctaccgc attatctacc tgcatgtgcc
     7621 tgcggcgatc tggtcgatgg gcatttatgc atcaatggca gtggcagcgt ttattggcct
     7681 tgtctggcag atgaaaatgg ccaacctggc ggtggcggcg atggccccca ttggtgccgt
     7741 gtttaccttt attgccctgg ttaccggctc tgcatgggga aaaccgatgt ggggcacctg
     7801 gtgggtatgg gatgcacgtc tgacttctga actggtgctg ctttttttgt atgtgggtgt
     7861 gattgccctg tggcacgcct tcgacgaccg ccgtctggcg ggccgtgcgg caggtatcct
     7921 ggtgctgatt ggcgtggtga atctgccgat tattcattac tccgtggagt ggtggaacac
     7981 cctgcatcag ggatcaacgc ggatgcagca aagtatcgat ccggcgatgc gttcgccgct
     8041 gcgctggtcg atttttggct tcctgctcct gtctgccacg ctgacgctga tgcggatgcg
     8101 taatttgatt ttgctgatgg aaaaacgccg tccgtgggtg agtgaactga tactgaaaag
     8161 aggccgtaaa tgacccctgc atttgcttcc tggaatgaat ttttcgcaat gggcggttac
     8221 gccttttttg tctggctggc ggtggtgatg accgttattc cgctggtggt tttggtcgtg
     8281 cactcggtga tgcagcatcg cgcaattctg cgtggtgtgg cgcaacagcg ggcgcgtgag
     8341 gcgcgtttac gtgctgcgca acagcaggag gctgcatgaa tattcgccgt aaaaaccgct
     8401 tgtggattgc ctgtgctgtt ctggcagggt tggcactgac tatcggtctg gtgctatatg
     8461 cgctgcgctc gaatatcgat ctcttttata cgccggggga aattctctac ggcaagcgtg
     8521 aaactcagca aatgccggaa gtcggtcagc gtctgcgcgt tggcgggatg gtgatgccgg
     8581 gtagcgtgca gcgcgatccc aattcgctaa aagtaacttt caccatttac gacgctgaag
     8641 gttcagtaga tgtctcttac gaaggcattt tgccggatct gttccgtgaa gggcagggcg
     8701 ttgtggtgca gggcgaactg gaaaaaggca atcatatcct cgcaaaagaa gtgctggcga
     8761 aacacgacga aaactacacg ccgccagaag ttgagaaagc gatggaagcc aatcaccgtc
     8821 gcccggcgag tgtttataag gacccagcat catgatgccg gaaattggta acgggctgct
     8881 gtgtctggcg ctaggaattg cgctgctgct gtccgtgtat ccgctatggg gcgtagcgcg
     8941 cggagatgcg cgcatgatgg cgtcttcccg cttgtttgcc tggctgctgt ttatgtctgt
     9001 ggctggcgca tttctggtac tggtcaatgc cttcgtggtc aacgacttca ccgtcaccta
     9061 tgttgccagc aactccaata cccagcttcc ggtgtggtat cgcgtggcgg ctacctgggg
     9121 cgcgcatgaa ggctcgctcc tgctgtgggt gctgctgatg agcggctgga cctttgcggt
     9181 agcgattttt agtcagcgta ttccgctgga tattgtggcc cgcgtactgg cgataatggg
     9241 gatggtcagc gtcggctttt tgctgttcat tctctttacc tctaacccgt tctcacgcac
     9301 gttgccgaac ttcccgattg aagggcgcga tcttaacccg ctgttacagg atccggggct
     9361 gatcttccat ccgcctctgc tctatatggg gtacgtgggg ttctcggtgg cgtttgcttt
     9421 tgccattgct tctttgttgt gcgggcgtct ggacagcact tatgcgcgtt ttactcgtcc
     9481 gtggacgctg gcggcgtgga tcttcttgac gctcggcatt gtgctcggtt ccgcatgggc
     9541 ctattacgaa ctcggctggg gcggctggtg gttctgggat ccggtagaaa acgcctcgtt
     9601 tatgccgtgg ctggtgggga ctgcgctgat gcactcactg gcggtcactg aacaacgcgc
     9661 cagcttcaaa gcgtggacat tactgctggc aatcagtgcc ttctcgttgt gtctgctggg
     9721 gactttcctg gtgcgttccg gcgtgctggt atcggtacac gcgtttgcct ctgatccggc
     9781 acgcggtatg tttatcctcg cctttatggt actggtgatt ggtggttcgc tgctgctgtt
     9841 tgccgcgcgt ggacacaaag ttcgctcacg cgtaaacaat gcgctgtggt cgcgggaatc
     9901 tctgctgtta gcgaacaacg ttttgctggt cgccgcgatg ctggtggtat tactggggac
     9961 gctgctgccg ctggtgcaca agcaactggg actgggcagt atttcgattg gcgaaccgtt
    10021 cttcaacacc atgtttacct ggctgatggt gccgtttgcg ctgctgcttg gtgtcggtcc
    10081 tctggtgcgc tgggggcgcg atcgcccacg taaaatccgc aatttattga ttatcgcctt
    10141 catctcaacg ctggtgctgt cgctgctgct gccgtggctg ttcgaaagca aagttgtggc
    10201 gatgacggtg ctcggcctgg caatggcctg ctggattgct gtgctggcaa ttgcggaagc
    10261 tgcgctgcgt atttcacgtg gcacgaaaac caccttcagt tattggggaa tggtggcggc
    10321 tcacctgggg ctggcagtga caattgttgg tattgccttt agccagaact atagcgttga
    10381 gcgtgatgtg cgcatgaagt ccggcgatag cgtcgatatt catgaatatc gcttcacctt
    10441 ccgtgatgtc aaagaggtga ctggcccgaa ctggcgtggc ggtgtggcga ctatcggcgt
    10501 aacgcgcgat ggcaagccgg aaacggtgct gtatgcggaa aaacgttatt acaacactgc
    10561 cgggtcgatg atgaccgaag cggcaattga cggcggcatc acgcgtgacc tgtacgccgc
    10621 gctcggtgaa gagctggaaa acggcgcgtg ggccgtgcgt ctttactaca aaccatttgt
    10681 tcgctggatt tgggcgggcg ggctgatgat ggcgttgggc ggactactgt gtctgtttga
    10741 tcctcgctat cgtaagcgcg tgagtccgca aaaaactgcg ccggaggccg tatgaagcgc
    10801 aaagtattgt taattccgtt gattatcttc ctggcgattg ccgcggcgct gctgtggcag
    10861 ctggcgcgta atgccgaagg ggatgatccg accaatctgg aatcggcgct cattggcaag
    10921 cctgtgccga agtttcgtct cgaatcactg gacaatccgg ggcagtttta tcaggcggat
    10981 gtgctgactc agggcaaacc agtactgctt aacgtctggg cgacctggtg tccgacctgc
    11041 cgtgcggaac atcaatatct gaatcagctt tcagcgcagg gcattcgcgt ggtcggcatg
    11101 aactataaag acgatcgcca gaaggcaatc agctggctga aagagctggg caatccttac
    11161 gcgctaagcc tgtttgatgg cgacggcatg ttagggctgg atctcggtgt ctatggcgcg
    11221 ccagaaacgt tccttattga cggcaacggc atcattcgct atcgccatgc gggcgatctc
    11281 aatcctcgcg tctgggaaga agagatcaag ccgctgtggg agaaatacag taaggaggcc
    11341 gcacaatgag gtttttattg ggcgtgctga tgctgatgat ctccggctca gcgctggcga
    11401 ccatcgacgt gttgcagttt aaagatgaag cacaggaaca acagttccgt cagctcactg
    11461 aagaactgcg ctgcccgaaa tgccagaata acagcattgc cgattccaac tcgatgattg
    11521 ccaccgacct gcgccagaaa gtgtatgaac tgatgcagga aggtaaaagt aagaaagaga
    11581 ttgtcgatta tatggtggcg cgttacggca acttcgtcac ttacgatccg ccgttaacgc
    11641 cgctgaccgt gctgctgtgg gtgctgccag tagtggctat tggcattggc ggttgggtca
    11701 tttacgcccg ctcgcggcgt cgggtacgcg tggtgccgga agcgtttcct gaacaaagcg
    11761 tgccggaagg taagcgtgcc ggatatgtcg cttatttgcc gggcattgtg gtggcgttaa
    11821 ttgtggctgg cgtcagctac taccagaccg gaaattatca acaggtgaaa atctggcagc
    11881 aggccacggc acaggctccg gcgttgctgg acagggcgct ggatccgaaa gccgatccgc
    11941 tcaacgaaga agagatgtcg cgtcttgcgc tggggatgcg tactcaactg caaaaaaatc
    12001 cgggagatat agaaggctgg attatgttgg gccgcgttgg catggcgctg ggtaacgcta
    12061 gtatcgccac cgatgcatac gctactgcgt atcgcctcga tccgaaaaac agcgatgccg
    12121 cactgggtta tgctgaagcg ttgacacgtt catctgatcc caacgacaac cgcctcggcg
    12181 gcgaactgct gcgccagttg gtgagaagcg accacagcaa tatccgtgtg ttaagcatgt
    12241 atgcgtttaa tgcctttgag cagcagcgat ttggcgaagc cgttgccgcg tgggagatga
    12301 tgttgaaact cttacctgcc aacgatactc gccgtgcggt gattgaacgt agtatcgcgc
    12361 aggcgatgca acatttgtcg ccgcaggaga ataaataaaa agaggcccga tggtgatgcc
    12421 atcgggctat ttttattgtg ccccgcgttg ttgcaggaac agaatggtgg ctgccacgcg
    12481 tgagcggaca ttgagtttgc gcagcagatt gcgaatatgt acttttactg tctgctctga
    12541 aatattcaac accgaggcaa tctgtttatt tgacagcccc tgtgccagct cgtgcagaac
    12601 atccagctcg cgctccgtca gcacgctgaa gggatcttct tccgcgccaa acatttcacg
    12661 ttcacgtaag tactgattga cgcgttcgct aaagactttg ctgcctttcg ctccagcacg
    12721 aattgcttcc agcaatactt ccgggtcgct gtctttcaac agataaccgt ctgcgcctgc
    12781 gtctatcagc gcaaagacat cgctggaggc atctgatacg gtcaggataa taatttgcgc
    12841 ggtaacgcca tccctgcgca aggcattgag ggtatccagg ccactcatac ctttcatatt
    12901 gagatccagc aagatcacgt cgatatccag tctattcgcc agatcgatcg cgctcgcgcc
    12961 gtcgcccgct tcggcgacca cttcaaagcc aggatcaagc tccagtaact gacgaacacc
    13021 gcgtcgcata agtggatgat catccacaat catcacctga aaaggcgttg cttcaggcat
    13081 agtagtctcc tgaggtttta ttagaatcat tatgtttgag cgtagcgttg gtaacgctac
    13141 caggcgcgtg aatgttaccc cattttatgt atggacatgt agtgaaaact acaaccttga
    13201 cgttcgcttt ggcatcattt tcattataaa gacggaattt tgcatcagct tctcatcaag
    13261 aaaaacaact aaaaaatcaa tcacaatact tgtgcatttc tttaaacagt ttttaaaaac
    13321 tgactcccgg tatggagcta tgggtatttt ctgtacccaa tgcttttaac agcaattaat
    13381 ttcataggat gaaagctcaa tgcatcaatc tggttctgtt tctctttgtc gttccgcaat
    13441 atctgttctg gtggctacag cgttatattc acccatagca ttggcatcaa cagttgagta
    13501 tggtgagaca gttgatggtg ttgtcctgga aaaagatatc cagctggttt atgggaccgc
    13561 caataatacg aaaatcaatc ctggcggaga acagcatata aaagaatttg gtgtaagtaa
    13621 taatactgaa attaacggag ggtatcagta cattgaaatg aatggcgccg cagaatactc
    13681 agtattaaat gacggttatc aaattgttca aatgggtggc gcggcaaacc agactacgct
    13741 caataatggt gtgctacagg tttatggcgc agcgaatgat accacgatta aaggcgggcg
    13801 cttaatcgtt gaaaaagatg ggggggccgt ctttgtcgct atcgaaaagg gaggactact
    13861 ggaggttaaa gaggggggat tagcgattgc ggtagatcag aaagcaggcg gtgctattaa
    13921 aacaaccacg cgggccatgg aggtattcgg aacaaaccgt ctcggtcagt tcgatatcaa
    13981 gaatggtatt gctaataata tgttgttgga aaacggcgga agtttgcgag ttgaagaaaa
    14041 tgacttcgct tataatacca ctgtagatag tggcggctta ctggaggtta tggatggcgg
    14101 gactgtaact ggcgttgata aaaaagcagg cggaaaatta attgtctcaa cgaatgcgct
    14161 ggaagtgagt ggtccaaaca gtaaaggcca atttagtata aaagatggtg tgtcaaaaaa
    14221 ttatgaactg gatgatggtt ccgggctcat tgttatggag gacacgcagg ccattgatac
    14281 tatccttgat gagcatgcca ctatgcaatc gctgggaaag gatactggta cgaaagtgca
    14341 ggcaaatgcg gtatatgatc tcggtcgatc atatcagaat ggaagtatca cgtattcctc
    14401 aaaagccatc tctgaaaata tggttatcaa caatggccgc gctaacgtct gggctggcac
    14461 aatggttaac gtttcagtca gagggaatga tggcattctt gaggtcatga agccgcaaat
    14521 aaattatgca cccgcaatgt tggtgggtaa ggtagtggtt tctgagggcg cttcttttag
    14581 aacgcatagt gccgtggata ccagcaaagc ggacgtttcg ctcgaaaata gcgtatggac
    14641 catcattgcc gatatcacta cgacgaacca aaacaccctc ctcaacttag ccaaccttgc
    14701 gatgtctgac gcaaatgtga ttatgatgga tgagccagtg actcgttcat cagtgacggc
    14761 aagtgcggaa aatttcatta cgttgaccac caataccctg tcgggaaacg gcaattttta
    14821 tatgcgtacc gatatggcta atcatcagag cgatcagctc aacgtcaccg gtcaggcaac
    14881 aggtgatttc aaaatattcg tgacggacac cggtgccagc ccggcagcag gagatagcct
    14941 tacactggta acaacgggcg gcggtgatgc tgcatttacg ttgggcaatg ccggaggcgt
    15001 tgttgatatc ggtacgtatg aatatacctt gctggataat ggcaaccata gctggagttt
    15061 ggcagagaat cgcgcgcaaa ttaccccttc aaccactgat gtgctgaata tggcggcagc
    15121 acaaccgctg gtatttgatg cagaactgga caccgtgcgt gagcgtcttg gtagcgtaaa
    15181 aggcgttaat tacgatacgg cgatgtggag ttcggcaatt aacacccgca acaacgtgac
    15241 cactgatgcg ggcgctggct ttgagcaaac attgaccggg ctgacgctcg gtatcgatag
    15301 ccgtttctcc cgtgaagaaa gcagcacaat tcgcggcttg ttctttggtt actctcattc
    15361 tgatattggt tttgatcgcg gcggcaaagg caatgtcgat agctataccc tgggggctta
    15421 tgccggttgg gagcatcaga acggtgccta tgttgatgga gtggtgaaag ttgaccgttt
    15481 tgccaacacc atccatggca agatgagtaa tggggcaaca gcgtttggcg attacaatag
    15541 taacggcgcg ggtgctcatg tcgagagcgg gttccgttgg gttgacggat tgtggagtgt
    15601 tagaccctat ctggccttta ccggctttac cacagatggt caggactaca cgttatcaaa
    15661 cggcatgcgc gcggatgtgg gaaatacccg gatattacgc gctgaagcgg gaacggcggt
    15721 aagctatcac atggacctgc aaaacggtac gacgctggaa ccctgactga aagccgccgt
    15781 gcgtcaggaa tacgccgatt ctaaccaggt gaaagttaat gacgatggca aatttaataa
    15841 tgatgtggct ggaacccgtg gcgtttatca ggctgggata aggtcatcgt ttaccccgac
    15901 gttaagcggt catttgtcag tcagctatgg caatggcgca ggggtagaat cgccgtggaa
    15961 tactcaggcg ggtgtggtct ggacgttctg ataacaaaaa ataaacaggc tgtgatgtgt
    16021 cacggtctgt ttatcgaatt aattgcagat ataaaaaaaa ccaaccgtaa aggttggttt
    16081 tttcttggga tttt
//
LOCUS       DAZGOB010000113        16013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGOB010000113 DAZGOB010000000
VERSION     DAZGOB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.113"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..159
                     /locus_tag="ACHLIF_003785"
     CDS             <1..159
                     /locus_tag="ACHLIF_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9607717.1"
                     /translation="LESDENGIIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGT
                     WENVGGMK"
     gene            563..1576
                     /gene="nleC"
                     /locus_tag="ACHLIF_003786"
     CDS             563..1576
                     /gene="nleC"
                     /locus_tag="ACHLIF_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO9607718.1"
                     /translation="MYKTILSINMKIPVLQPGFNFFAPAGYSAAVAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEEVHIGCIKYRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYADPEREAHLRLRNLNALRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
     gene            complement(1697..1780)
                     /locus_tag="ACHLIF_003787"
                     /pseudo
     CDS             complement(1697..1780)
                     /locus_tag="ACHLIF_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            1865..1951
                     /locus_tag="ACHLIF_003788"
                     /pseudo
     CDS             1865..1951
                     /locus_tag="ACHLIF_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(1948..2196)
                     /locus_tag="ACHLIF_003789"
     CDS             complement(1948..2196)
                     /locus_tag="ACHLIF_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO9607719.1"
                     /translation="MRVEICIAKEKISKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2422..2517)
                     /locus_tag="ACHLIF_003790"
                     /pseudo
     CDS             complement(2422..2517)
                     /locus_tag="ACHLIF_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2514..3080)
                     /gene="yecD"
                     /locus_tag="ACHLIF_003791"
     CDS             complement(2514..3080)
                     /gene="yecD"
                     /locus_tag="ACHLIF_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO9607720.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            3390..5162
                     /gene="aspS"
                     /locus_tag="ACHLIF_003792"
     CDS             3390..5162
                     /gene="aspS"
                     /locus_tag="ACHLIF_003792"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO9607721.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            5280..5732
                     /gene="nudB"
                     /locus_tag="ACHLIF_003793"
     CDS             5280..5732
                     /gene="nudB"
                     /locus_tag="ACHLIF_003793"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO9607722.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            5761..6501
                     /gene="yebC"
                     /locus_tag="ACHLIF_003794"
     CDS             5761..6501
                     /gene="yebC"
                     /locus_tag="ACHLIF_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9607723.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            6536..7057
                     /gene="ruvC"
                     /locus_tag="ACHLIF_003795"
     CDS             6536..7057
                     /gene="ruvC"
                     /locus_tag="ACHLIF_003795"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO9607724.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(7059..7661)
                     /gene="yebB"
                     /locus_tag="ACHLIF_003796"
     CDS             complement(7059..7661)
                     /gene="yebB"
                     /locus_tag="ACHLIF_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO9607725.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            7732..7797
                     /gene="yobI"
                     /locus_tag="ACHLIF_003797"
     CDS             7732..7797
                     /gene="yobI"
                     /locus_tag="ACHLIF_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO9607726.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            7936..8547
                     /gene="ruvA"
                     /locus_tag="ACHLIF_003798"
     CDS             7936..8547
                     /gene="ruvA"
                     /locus_tag="ACHLIF_003798"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO9607727.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            8556..9566
                     /gene="ruvB"
                     /locus_tag="ACHLIF_003799"
     CDS             8556..9566
                     /gene="ruvB"
                     /locus_tag="ACHLIF_003799"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO9607728.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(9713..10498)
                     /gene="znuB"
                     /locus_tag="ACHLIF_003800"
     CDS             complement(9713..10498)
                     /gene="znuB"
                     /locus_tag="ACHLIF_003800"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO9607729.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(10495..11250)
                     /gene="znuC"
                     /locus_tag="ACHLIF_003801"
     CDS             complement(10495..11250)
                     /gene="znuC"
                     /locus_tag="ACHLIF_003801"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO9607730.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            11329..12261
                     /gene="znuA"
                     /locus_tag="ACHLIF_003802"
     CDS             11329..12261
                     /gene="znuA"
                     /locus_tag="ACHLIF_003802"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO9607731.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            12277..13599
                     /gene="mepM"
                     /locus_tag="ACHLIF_003803"
     CDS             12277..13599
                     /gene="mepM"
                     /locus_tag="ACHLIF_003803"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO9607732.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQATQEDEAIPLDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            13719..14690
                     /gene="lpxM"
                     /locus_tag="ACHLIF_003804"
                     /gene_synonym="msbB"
     CDS             13719..14690
                     /gene="lpxM"
                     /locus_tag="ACHLIF_003804"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO9607733.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(14779..>16013)
                     /locus_tag="ACHLIF_003805"
     CDS             complement(14779..>16013)
                     /locus_tag="ACHLIF_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HFO9607734.1"
                     /translation="SSYKDPHILFNTTCFARNEWININNNWLKARVNSYGYAVIDPKN
                     KIVNGLKAESRSIENNYIKLLFSESGDLISLYDKRYGKEYITENMHSEIRAYHEDAGF
                     FAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIYHYNSSYLRFAFTLTQDSPRV
                     DVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDRPTHSDDSFAFAKDEIPAHH
                     WVDLSDQETGIALIAKNKYGYRVKDQTLELTLLRSQHKPGEVVKTDNYDNFLINNFAD
                     IGKQKFTFSLFPHHGDYRVGGVVNQAYIMNHPLQIVPVKPHPGELPPSLSFFAIDTNS
                     VIIETIKLAEDGDGIIVRLYESYGLEISAMFSWVCNYHYVQYVDLQENKLDDSFYCNQ
                     YCNLEFKPFEIITLRLTQ"
BASE COUNT         4228 a         3728 c         4090 g         3967 t
ORIGIN      
        1 ctggagtcag atgagaatgg cattatcccg gagcaggaca gggtaatcac gcaggtggtg
       61 atactggatg cggataaaaa gcagatacag tgcgtggtaa gaccgctgca aatcctgcgt
      121 gctgacggga cgtgggaaaa tgttggcggg atgaaatagc ccgacagctt cacaaaaacc
      181 ggagtccggc tccggttttt gtgttgcaat gtccgtggga tatttgttaa gtagatgata
      241 gaggtgctaa ttcaacaggg agataaatta atgccgataa atctgacatc ttatttgggg
      301 ttacaggggg cgaaagttgt cccggcagtt gttttttcta aaatttagtc ttgttggggt
      361 aaatgacata tgccattgat aacatccagc atttcatcaa atgttacttt gcagagtatt
      421 gaaatattac gtgaagctgc cagacaaaac ctgataacga aagatattac tataaatggg
      481 cagaaagttg gtattcatta ttatcaacgc cctgacgttt ttttagtttc tggttgtaag
      541 cgagtgaatt ggtggcactg aaatatataa aaccatatta agtatcaata tgaaaattcc
      601 cgttctccaa cctggcttca acttttttgc ccctgctgga tactctgctg ccgttgctcc
      661 taatcgtgct gaaaatgcct atgcggatta cgttttggat ataggtaagc gaataccgct
      721 ttccgcagca gatttaagca acgtatacga aagtgtaatt cgcgccgtcc atgacagccg
      781 tagcaggctc atcgatcagc atacggtcga tatgattggc aacactgtac ttgatgcgtt
      841 gagccgatca caaacatttc gtgatgccgt aagctatggc attcataatg aggaggtaca
      901 cattggttgc attaaataca gaaacgaata cgagcttaac ggagaatctg ctatcaaaat
      961 tgatgatatt caatcactaa cctgtaacga attatatgga tacgatgtcg ggcaagaacc
     1021 aattctcccc atttgcgagg caggagaaaa cgagaacgaa gagccttatg tcagttttag
     1081 tgttgcgcca gatactgatt cttatgagat gccatcgtgg caggaaggac tgattcacga
     1141 gattattcat catgttactg gggccagcga tccatctgga gatagtaata tagagctagg
     1201 acccaccgag attctcgcac gtcgtgtcgc tcaagagctg ggatggagtg ttcccgactt
     1261 caaaggatat gcagatccag aacgagaagc tcatcttaga ctacgtaacc tgaatgccct
     1321 tcgacaggct gccatgaggc atgaaaagaa tgagagggct ttcttcgaaa gactgggtac
     1381 gatcagtgac cgatatgagg cgagtcctga tttcacagag tattccgctg tgtctaacat
     1441 aggatacgga tttatccagc aacatgattt tccaggggtg gctatcgacg agaatttaca
     1501 ggatgtaaat cagatccaac tgtatcatgg tgctccttat atctttacat ttggggatgt
     1561 ggacagacac aattagcgct gattcgtctt tacagttaca tagggttact actcatagat
     1621 attaacggag ttgatgatgg gcaatcgtgc aacattgtat tgtatgtaaa ggcagagccc
     1681 cgaattttac tgttgtaagt gggcctggcg ggaatggtat cgccagcatc attggcttgg
     1741 gtccagtaaa aagagacgca gaaagatgca caataggcac tacgcgtcat gcatggatta
     1801 ggtcgctcat catttacaca gtactgctaa aaaattatta aggaaagcgc ggcacttcgt
     1861 atgtaagaac gtgtcgcggt tggctggtga actttcgata gtgcgagtat tgaatgattt
     1921 ccagccgtta ccgattttac gtgtttatta gtgaacaaac cactcgtcag cagactccca
     1981 ggtatctttc agagtctcct gaacaaatgt ttttgcagaa tctttatctg cggtgcgtgt
     2041 aacagaaagc ccatcgttgc tggtggcttt tacgatcacc tctacatcgt cataacgttt
     2101 actgatgcgt cgggttaatt cttcctttaa cgcatccaca gcaccgtttg gcattttgct
     2161 gattttttct ttagcaatac agatctcaac acgcataaca ccctcctgta actgtggtta
     2221 tatacaggtg ttatttttat ctgtatggat aaccagtgtc aattcctgat attgtttatg
     2281 gggcatcaat ggggcatgta tgggacactt tttatcggcg aaattcgtcg aagttcgtcg
     2341 acatggtaaa cgaatcatct atccaaccct tgaaaaacgg cgctcctgga cgatcttcgt
     2401 cgatttttaa aaatgttgcg tcatgcagtt aaagtggcgg gcatactctt caaggctggt
     2461 gatccccaac cgcacccatt taggatgcga ccattgcggt agaccgatgt aaatcataac
     2521 gcgtgaagga tctcttccac gctacgcaca cgggcgatgc gtgggtagat atggttgatg
     2581 ctgttattgt gctgctcggc gctagcggca ctacaggcgt cttcggcaat caccagatta
     2641 aagccaagtt cccaggcatt gcgggcggtg gattcaacac cgatattggt cgagatccca
     2701 cataacacta ttgtatcgat acctcggcgg cgcagttgca actccagatc cgtaccgtaa
     2761 aacgcacccc actgacgttt gatgatttcg atatcactgt cagttgcacc taatgcagct
     2821 ggatgttgcc accagttttc gggcaacact ttagcggggg agggggcatc aaccggctgt
     2881 tttaatgctt cggcgtaatc ggcagaccag ccaacgcgca ccagaaacac gggctgaccg
     2941 ctggcgcgaa atttcgccgc cagcttcccg gcgcgattaa ctacctcatc ggcagtatgt
     3001 ggaccaccgg caaaaggtaa gatgccttct tgtaaatcga tcaccaccag cgcagtggtt
     3061 ttagcattaa gttcaagcat aatgactcct gaaaaaacaa aatcgtgcct cacaccttaa
     3121 tgcgattagc cgatggcgga acgacaaatt ttgttaattt ttatgagttc gggcaacacc
     3181 tttatagcaa actggcgtag agtctgattt caggcccgat ataaagtggt aacgaggcgt
     3241 tccagcgcgg gccaggctct tacggtgcgg cagaatttcc agtataatag ccgccttttt
     3301 tcattcagtt gtgacataca gctaacgctg cgacttggtc acctgcgcag cagggggcat
     3361 ccgccggcag ctaagttaag ggatatctca tgcgtacaga atattgtgga cagctccgtt
     3421 tgtcccacgt ggggcagcag gtgactctgt gtggttgggt caaccgtcgt cgtgatcttg
     3481 gtagcctgat cttcatcgat atgcgcgacc gcgaaggtat cgtgcaggta tttttcgatc
     3541 cggatcgtgc ggacgcgtta aagctggcct ctgaactgcg taatgagttc tgcattcagg
     3601 tcacgggcac cgtacgtgcg cgtgacgaaa aaaatattaa ccgcgatatg gcgaccggcg
     3661 aaatcgaagt gctggcgtcc tcgctgacta tcatcaaccg cgcagatgtt ctgccgcttg
     3721 actctaacca cgtcaacacc gaagaagcgc gtctgaaata ccgctacctc gacctgcgtc
     3781 gtccggaaat ggctcagcgc ctgaaaaccc gcgctaaaat caccagcctg gtgcgccgtt
     3841 ttatggatga ccacggcttc ctcgacatcg aaactccgat gctgaccaaa gccacgccgg
     3901 aaggcgcgcg tgactacctg gtgccttctc gtgtgcacaa aggtaaattc tacgcactgc
     3961 cgcaatcccc acagttgttc aaacagctgc tgatgatgtc cggttttgac cgttactatc
     4021 agatcgttaa atgcttccgt gacgaagacc tgcgcgctga tcgtcagcct gaatttactc
     4081 agatcgatgt ggaaacttct ttcatgaccg cgccgcaagt gcgtgaagtg atggaagcgc
     4141 tggtgcgtca tttgtggctg gaagtgaagg gcgtggatct gggcgatttc ccggtaatga
     4201 cctttgcgga agcagaacgc cgttatggtt ctgataaacc ggatctgcgt aacccgatgg
     4261 aactgaccga cgttgccgat ctgctgaaat ctgttgagtt tgcagtattt gcggggccgg
     4321 cgaacgatcc gaaaggccgt gttgccgctc tacgtgttcc gggtggcgca tcgctgaccc
     4381 gtaagcagat cgacgaatac ggtaacttcg ttaaaatcta cggcgcgaaa ggtctggctt
     4441 acatcaaagt taacgaacgc gcgaaaggtc tggaaggtat caacagcccg gtagcgaagt
     4501 tccttaatgc agaaatcatc gaagccatcc tggatcgtac tgccgcgcaa gatggcgata
     4561 tgattttctt cggtgccgac aacaagaaaa ttgttgccga cgcgatgggc gcactgcgcc
     4621 tgaaagtggg taaagacctt ggtctgaccg acgaaagcaa atgggcaccg ctgtgggtta
     4681 tcgacttccc gatgtttgaa gacgacggtg aaggcggcct gacggcaatg caccatccgt
     4741 tcacctcacc gaaagacatg actgctgcag aactgaaagc tgcaccggaa aatgcggtgg
     4801 cgaacgctta cgatatggtc atcaatggtt acgaagtggg cggtggttca gtacgtatcc
     4861 ataatggtga tatgcagcag acggtgtttg gtattctggg tatcaacgaa gaggaacagc
     4921 gcgagaaatt cggcttcctg ctcgacgccc tgaaatacgg tactccgccg cacgcaggtc
     4981 tggcattcgg tcttgaccgt ctgaccatgc tgctgactgg caccgacaat atccgtgacg
     5041 ttatcgcctt cccgaaaacc acggcggcag cgtgtctgat gactgaagca ccgagctttg
     5101 ctaacccgac tgcactggct gagctgagca ttcaggttgt gaagaaggct gagaataact
     5161 gatatgactc aaatacacga aatcattcgc gttgcatcaa ggcggcaact gagtgaactc
     5221 ccatgagcat agataactat gtgaatggga tgagcgaagg cagtcaacga agaggcagcg
     5281 tgaaggataa agtgtataag cgtcccgttt cgatcttagt ggtcatctat gcacaagata
     5341 cgaaacgggt gctgatgttg cagcggcgtg acgatcccga tttctggcag tcggtaaccg
     5401 gcagcgtgga agagggtgaa accgcgccgc aagctgccat gcgcgaagta aaggaagagg
     5461 tcaccattga tgttgtcgct gaacaactga ccttaattga ctgtcagcgc acggtagagt
     5521 ttgaaatttt ttcacattta cgtcatcgct atgcgccggg cgtgacgcgt aatacggaat
     5581 catggttctg tcttgcgctt ccgcacgagc ggcagatcgt tttcactgaa catctggctt
     5641 acaagtggct tgatgcgcct gctgcggcgg cgctcactaa gtcctggagc aaccggcagg
     5701 cgattgaaca gtttgtcatt aacgctgcct gaacaggcag ggctatctgg agatattttt
     5761 atggcaggtc atagtaaatg ggccaacacc agacatcgta aagctgcgca ggatgcaaag
     5821 cgcggtaaaa tcttcactaa aatcattcgt gagctggtaa ccgcggctaa gctgggcggt
     5881 ggcgatccgg acgctaaccc gcgtctgcgt gcagcaattg ataaagcact gtctaacaac
     5941 atgacccgcg acacactgaa ccgcgcaatt gcacgtggtg tgggcggtga tgatgatgca
     6001 aacatggaaa ccatcatcta cgaaggttac ggtcctggcg gcacggcaat catgattgaa
     6061 tgtctgtctg acaaccgcaa ccgtaccgtt gctgaagtgc gtcatgcatt cagcaaatgt
     6121 ggcggtaacc tcggtactga tggttccgta gcctatctgt tcagcaaaaa aggcgtgatc
     6181 tccttcgaga aaggcgatga agacaccatc atggaagcag ctttggaagc aggtgctgaa
     6241 gacgttgtga cctatgatga cggcgcgatt gatgtctaca ccgcatggga agaaatgggt
     6301 aaagtgcgcg acgctctgga agcggcaggt ctgaaagcag acagcgcgga agtttccatg
     6361 atcccgtcca ccaaagctga tatggatgca gaaaccgcac cgaaactgat gcgtctgatc
     6421 gatatgctgg aagattgcga cgacgtgcag gaagtttacc ataacggtga aatctctgat
     6481 gaggttgcag cgactctctg atgaggcctg ctaaacagca aaacggagac gcgtgatggc
     6541 tattattctc ggcattgatc cgggttcgcg cgtgaccggc tatggcgtca tccgccaggt
     6601 aggcaggcaa ctgtcctacc tgggtagtgg atgcatacgc accaaagtgg atgatttacc
     6661 gtctcgtctg aagctcatct atgcgggcgt gacggaaatc atcacccagt tccagcctga
     6721 ttatttcgcc attgaacaag tctttatggc gaagaacgct gactcagccc tgaaactggg
     6781 ccaggcgcgc ggcgtggcga ttgtggcggc ggtgaatcag gaattgccgg tatttgaata
     6841 cgcggcacgt caggtaaagc aaacggtggt aggtattggt agcgccgaga aaagccaggt
     6901 gcagcatatg gtccgcacct tgctgaaact gcccgctaat ccacaagcgg atgccgctga
     6961 tgcgttagct atcgcgatta cccattgtca cgtcagccag aatgcaatgc agatgagcga
     7021 atcgcggctg aacctggcga gagggcgact gcgttaagtt ataccgcctc ggtcagttca
     7081 ggctgaggcg tttcaactcc ctccgcgtga atcaacacca aacccggatg atgccacaaa
     7141 ctggctggcg tgacggtttt acgctcccac ggaatagaac ctaagaacca gaatttccag
     7201 aaagtgagtt ttgcattcgg attgctattt aacagttgtc caaacgtctc tatttcaccc
     7261 accggaatac atagcgcctc tttataaata tcaaagacaa attttgaaca gaactggcgg
     7321 gaagattcgt atttaaaacc cgtgtggtaa agtttgcgta gccgggaggg aacctgttca
     7381 acaattcgtt gtttctgttg ttctgtcagc ccggcgtcta atcgctttat agcatagcgt
     7441 tgattagcag agcgtttaat aaaacgggat agcgtagtga tggttgagag gggaacacgg
     7501 ctttctgcaa ccagaaagtc ttcaccgtta tgaccgataa tgatcccgac gtgattactc
     7561 cagcagtttg atgcagctga tatttgacca aataaggcag cacctataca tgtaaagacg
     7621 atatcaccaa tttcatattc agcagggtaa ttaatattca cgttgtcatt ccattgaaat
     7681 agatatgaat atacgtaata gcataaaaaa tttattattt caggagtcgc aatgtatatt
     7741 tttattacgc atttcttcac tgaatatgta atattcaaat atttgcttcc aatataacct
     7801 gtagaataaa ttatactgcg ccatttttca gatcgttggg acgcctcgca agttttcttc
     7861 atccttcgct ggatatctat ccagcatttt tttatcatac agcattatct ttgattcatt
     7921 acgcaggagc gtcatgtgat aggcagactc agaggcatca tcattgaaaa acaacccccg
     7981 ctggtgttaa ttgaagtggg cggcgtaggc tatgaagtgc atatgccgat gacctgtttt
     8041 tatgaactcc ctgaagcggg tcaggaagcg atcgttttca cccactttgt ggtgcgtgaa
     8101 gacgcgcaac tgctgtacgg ttttaacaat aaacaagagc gcacattgtt caaagagttg
     8161 atcaaaacca acggcgtcgg cccgaagttg gcgctggcga tcctctccgg aatgtcagcg
     8221 cagcagttcg ttaatgccgt tgagcgtgaa gaagtggggg cactggtgaa actgccgggt
     8281 attggcaaaa aaaccgccga acgcttgatt gttgaaatga aagaccgatt taaaggtttg
     8341 catggcgatc tctttacgcc agccgccgac ctggtactca cgtcacctgc cagcccgacg
     8401 accgacgatg ctgaacagga agcggttgcc gcgctggtgg cgctgggcta taaaccacaa
     8461 gaagcaagcc gcatggtgag caaaatcgct cgccctgacg ccagcagtga aacattaatt
     8521 cgcgaagccc tacgcgccgc gttatgaggt aaaggatgat tgaagcagac cgtctgattt
     8581 ctgccggtac cactttgccg gaagatgtgg cagatcgcgc cattcgccct aaattactgg
     8641 aagagtatgt tggtcagccg caggttcgtt cacagatgga gattttcatc aaagcagcga
     8701 aactgcgcgg cgatgccctc gatcatctgt tgattttcgg tcctccgggg ttgggtaaaa
     8761 ctacgcttgc caacattgtc gccaatgaaa tgggcgttaa tttacgcacg acttctggtc
     8821 cggtgctgga aaaggcgggc gatctggcag cgatgctcac taaccttgaa ccgcatgacg
     8881 tgttgtttat tgatgagatc caccgtcttt cgccagtggt ggaagaggtg ttgtatccgg
     8941 caatggaaga ctaccaactg gatatcatga ttggtgaagg tccggcggca cgctccatta
     9001 aaattgattt gccgccgttt accctgattg gtgcaaccac gcgcgcaggt tcgctgacat
     9061 caccgttgcg cgatcgtttt ggtattgtgc agcgtctgga gttttatcag gtgccggatc
     9121 tgcaatatat cgtcagtcgc agcgcacgct ttatggggct tgagatgagt gatgacggcg
     9181 cgctggaagt tgctcgtcga gctcgcggta cgccgcgcat tgccaaccgt ctgctgcgtc
     9241 gagtgcgtga tttcgccgaa gtgaagcacg atggcaccat ctcggcagat atcgctgctc
     9301 aggcgttgga tatgttgaat gtcgatgctg aaggtttcga ttatatggac cgcaagttat
     9361 tgctggcggt aatcgataag ttctttggtg ggccggtagg tctggataac cttgcggcag
     9421 ccattggcga agaacgtgaa accattgagg atgtgctgga accttatttg attcagcaag
     9481 gctttttgca gcgtacaccg cgtgggcgta tggcgacgac gcgggcgtgg aatcactttg
     9541 gcataacgcc gccagaaatg ccgtaagtcg gattggtcgc tcgcgccgca tccgacactg
     9601 gcagtgtgtc tgatgcgaca ctgttcgcgt cttatcaggc cagccagaga tagcgaaccg
     9661 taggtcggat aaggcgctcg cgccgcatcc gacaaatgtg ttcagcgata gattagctgg
     9721 cctgcttttt catcatactg agaataaata acagtgccgc acatagcacc accgacggac
     9781 ccgccggcgt atcgtaaaac gcggaaaagg ttaaaccgcc agttactgcc accatcccca
     9841 ccaaaacagc gacaccagcc atctgttccg gcgtacgggc aaagcgacgc gcagtagctg
     9901 caggaataat cagcagtgaa gtaataatca acgcgccaac aaatttcatc gctacaccaa
     9961 tcgtcaatgc cgtcaccagc atcaacaaca atttcacgcg ctgtaatttc acaccatcaa
    10021 caaacgccag atccgggcta atcgtcatcg acagcaaatt gcgccattgc cagaacaaaa
    10081 tagccaccac gatgaccacg ccaatcgcaa tagcgatgag atcttctggc gtcactgcca
    10141 gcaaatcacc gaacaggtaa gccatcaaat caacacgaat gttagacatc agactaacga
    10201 ccaccaggcc cagcgacagg gcactgtgcg ccataatccc taataacgtg tcaatcgcca
    10261 gctgtggacg cttctccagc cataccagac cgcccgccag gagcagcgta acggcaatta
    10321 ccgcatagaa tggattcacg tccagcaaca aaccaaacgc gacgccaagt aatgaggcat
    10381 gagccagcgt atcaccgaaa taagacatac gacgccagac tacaaacgaa cccagcggac
    10441 ccgcggcaca ggcgagcatg atcccggcta accaaccggg aaataataat tcaatcatga
    10501 gcgatcattt ccccgacgca aaacaattcg tccctgtaaa tcgtgacgat gattatgatg
    10561 atggcgatag atacccagtt gttcagcacc acgaggacca aacatagaaa taaactccgg
    10621 atgtagggaa acaacttccg gtgtgccgga acaacaaatg tggtgattca gacacagcac
    10681 ttcatcggtt tttgccatta ccagatgcag atcgtgagag accattaaaa cgccacaatc
    10741 cagttcgcga cgcaactggt caataaggtc atataacgcc acctggccat ttacatccac
    10801 gccttgagtg ggttcatcca gcactaataa ttgcggtcga tttaacaatg ctcgcgctaa
    10861 cagtacacgc tgtgtttcgc caccagagag cttttgcatc ggtgcgttaa tcagatgccc
    10921 ggcctggaca cgtttcagtg caggcaaaat atcttcttta tgtgtgccag ggcgtaagcg
    10981 taaaaaacgg tttacggtca gtggcaacgt ggtgtcgaga tacagcttct gcggtacata
    11041 gccgatgcgt agttttccgt tgcgcttgat aaccccttca tcgggtgtta ccagcccgag
    11101 cactacccgt accagtgtcg acttacctgc gccatttggc ccaagtaaag tcaaaatttt
    11161 tccaggttta agttccagcg acacatcaga gaggacgcgg cgttggccaa aagaaaccga
    11221 gacattttcc agggaaacca gacttgtcat gttaatttta gtcttgcagt agtcatgaaa
    11281 tgttataata tcacacttct catattcatt acgatgattg gtcgcattat gttacataaa
    11341 aaaacgcttc ttttcgcagc attatccgcc gctctctggg gaggtgcaac acaggccgca
    11401 gatgccgccg ttgtcgcttc gcttaaaccc gttgggttca tcgcttctgc cattgctgat
    11461 ggtgtaacag aaacggaggt tttactgcct gacggcgctt cagaacatga ttattcactg
    11521 cgtccatcgg atgtaaaacg cttacagaac gcggacttag tcgtttgggt tggcccggag
    11581 atggaagcgt ttatgcaaaa accggtaagc aaattaccag gagcgaagca ggtaacgatt
    11641 gcgcagcttg aagatgtgaa accgctactg atgaaaagta ttcacggcga tgatgatgat
    11701 cacgaccacg cggaaaaaag tgacgaagat caccatcacg gcgatttcaa catgcatctt
    11761 tggctttccc cagagatagc gcgggctaca gcggttgcaa tccatggaaa attagtggaa
    11821 cttatgccgc aaagtcgagc caaacttgac gccaacctga aggattttga ggcacaatta
    11881 gcctcaaccg aaacgcaggt tggtaacgag ctcgcgccgc tcaaggggaa aggttatttc
    11941 gtttttcacg atgcttacgg ctatttcgaa aaacagtttg gactgacacc gcttggtcat
    12001 tttaccgtta accctgagat tcaacctggc gcgcagcgtt tacatgaaat aagaacacag
    12061 ttggttgagc aaaaagcaac ctgcgttttt gctgagccac agttcaggcc agcggtcgtt
    12121 gagagcgtcg cacgagggac atccgttcgt atgggaacgt tggatcccct ggggacgaat
    12181 atcaaactgg gtaaaacaag ctattcagaa ttcctgagtc aattagccaa ccagtatgcg
    12241 agctgcctga aaggagatta atgaggaagt gattacgtgc aacagatagc ccgctctgtc
    12301 gccctggcgt ttaataattt accgcgacca caccgcgtta tgttggggtc gctcaccgtt
    12361 cttactctgg ccgtcgctgt ctggcggcct tatgtttatc accgtgatgc cacgccaatt
    12421 gtcaaaacca ttgagctgga acagaacgaa attcgttcgc tcttacctga agccagtgag
    12481 ccgattgatc aagctacaca agaagatgaa gccattcccc tggacgaact ggatgacaaa
    12541 atcgccggtg aagcgggcgt gcatgaatat gttgtttcca ctggcgatac gctaagcagc
    12601 attctcaatc agtatggtat tgatatgggt gatatcaccc aactggctgc ggccgacaaa
    12661 gaattgcgta acctgaaaat cggtcaacaa ctctcctgga cattaaccgc ggacggcgaa
    12721 ctgcagcgcc tcacctggga agtgtctcgt cgtgaaaccc gaacctatga ccgtactgcc
    12781 gctaacggtt ttaaaatgac cagcgaaatg cagcaaggag agtgggttaa caatctgctg
    12841 aaaggtaccg tcggggggag ctttgttgcc agcgccagaa acgccggttt aaccagcgcc
    12901 gaagtgagcg cagtgattaa agccatgcag tggcaaatgg atttccgcaa actgaaaaaa
    12961 ggcgatgaat ttgcggtgtt aatgtctcga gaaatgcttg atggtaaacg tgagcaaagc
    13021 cagctgctgg gcgtacgttt gcgttcagaa ggtaaagatt attacgctat ccgcgctgaa
    13081 gatggcaaat tctacgatcg taacggtact ggtctggcga aaggattctt gcgattcccg
    13141 acggcgaaac agttccgtat ctcgtctaac tttaacccgc gtcgaactaa tccggtgaca
    13201 ggtcgcgttg caccacacag aggtgttgat ttcgccatgc cacaaggtac gccagtgctt
    13261 tcagtgggtg acggtgaagt ggtggttgcc aaacgcagtg gcgcagcagg ttattatgtg
    13321 gctattcgtc atggtcgcag ctacaccacg cgttatatgc acttgcgcaa gattctggtg
    13381 aaaccgggac agaaggtgaa acgtggcgac cgtatcgcgc tttccggtaa taccggacgt
    13441 tcaactgggc cgcatctgca ctatgaagta tggataaacc agcaggccgt aaacccgctg
    13501 acggcaaaac tgccgcgtac tgaagggctg accggctccg atcgtcgcga attcctggcg
    13561 caggccaaag agattgtgcc gcagctacgg tttgattaat taacatccat tcgcagccgg
    13621 tacgcagtca gtaccggctt tttttatttg gtgcggggca agttgcgccg ctacactatc
    13681 accagattga tttttgcctt atccgaaact ggaaaagcat ggaaacgaaa aaaaataata
    13741 gcgaatacat tcctgagttt gataaatcct ttcgccaccc gcgctactgg ggagcatggc
    13801 tgggtgtagc agcgatggcg ggtatcgctt taacgccgcc aaagttccgt gatcccattc
    13861 tggcacggct gggacgtttt gccggacgac tgggaaaaag ctcacgccgt cgtgcgttaa
    13921 tcaatctgtc gctctgcttt ccagaacgta gtgaagctga acgcgaagcg attgttgatg
    13981 agatgtttgc caccgcgccg caagcgatgg caatgatggc tgagttggca atacgcggac
    14041 cggagaaaat tcagccgcgc gttgactggc aagggctgga gatcatcgaa gagatgcggc
    14101 gtaataacga gaaagttatc tttctggtgc cgcacggttg ggccgtcgat attcctgcca
    14161 tgctgatggc ctcgcaaggg cagaaaatgg cagcgatgtt ccataatcag ggcaacccgg
    14221 tttttgatta tgtctggaac acggtgcgtc gtcgctttgg cggtcgtctg catgcgagaa
    14281 atgacggtat taaaccattc atccagtcgg tacgtcaggg gtactgggga tattatttac
    14341 ccgatcagga tcatggccca gagcacagcg aatttgtgga tttctttgcc acctataaag
    14401 cgacgttgcc cgcgattggt cgtttgatga aagtgtgccg tgcgcgcgtt gtaccgctgt
    14461 ttccgattta tgatggcaag acgcatcgtc tgacgattca ggtgcgccca ccgatggatg
    14521 atctgttaga ggcggatgat catacgattg cgcggcggat gaatgaagaa gtcgagattt
    14581 ttgttggtcc gcgaccagaa caatacacct ggatactaaa attgctgaaa actcgcaaac
    14641 cgggcgaaat ccagccgtat aagcgcaaag atctttatcc catcaaataa aaaacagcct
    14701 ctcaaattat tgagaggctg tacttgataa aaaatctcaa tgagtgttct caccattttg
    14761 agtttttaga ttagtacgct attgagttaa tcgtagcgtg ataatttcaa aaggtttgaa
    14821 ttcaagattg caatattgat tgcaataaaa actgtcatct aatttatttt cttggagatc
    14881 gacatattga acgtagtgat aattacatac ccacgaaaac atggctgaaa tttctaatcc
    14941 ataactctca tataatctta cgattatacc gtcgccgtct tcagctaatt ttattgtttc
    15001 gatgataacg ctgttagtat cgatagcaaa aaatgatagt gaagggggta gttcacccgg
    15061 gtgtggtttg acagggacga tctgtaaggg atgattcatt atataggcct ggttaacgac
    15121 accacccaca cggtaatctc catgatgagg aaataaggag aatgtgaatt tttgtttacc
    15181 aatatctgca aaattattaa tcaggaaatt atcataatta tctgtcttaa caacttcccc
    15241 aggtttgtgc tgcgaacgca acaaagttaa ctctagcgtc tggtctttga cacgatagcc
    15301 atatttgttt tttgctatta aagcaatgcc agtttcttga tcagaaaggt cgacccagtg
    15361 atgtgctggt atttcatctt tggcaaaagc gaacgaatca tcgctatgcg ttggtctgtc
    15421 aattacaccg aactgaattt gacattgtgc aagagatgtt tgtactgata ccgggaattt
    15481 tactttgaga gaaacattag gttcgtgcca atcaataaaa gtttggacat caacgcgtgg
    15541 actgtcctgt gtcagggtaa atgcgaagcg aagataagag gaattgtagt gataaatcag
    15601 cgtcatcgta gttttaggcc cgctgatcac agttgtcatt ttttcagcga gtaaaacata
    15661 tgattcccca tcacgataat ttgaagcaaa atcccaggcc gcgaaaaaac ctgcatcttc
    15721 gtggtatgca cggatctcac tgtgcatatt ttcagtaatg tattctttac catatctttt
    15781 atcatatagc gaaattaaat caccgctttc tgaaaaaaga agtttaatgt aattattttc
    15841 aatacttcga gattctgcct tcagaccatt tacgatctta ttctttggat caatcacagc
    15901 ataaccataa gagtttactc gtgctttgag ccagttgttg tttatattga tccattcgtt
    15961 acgagcaaaa caggtggtat taaataaaat gtgcggatct ttataagatg aag
//
LOCUS       DAZGOB010000114        15801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGOB010000114 DAZGOB010000000
VERSION     DAZGOB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.114"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..677)
                     /locus_tag="ACHLIF_003806"
     CDS             complement(<1..677)
                     /locus_tag="ACHLIF_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO9607735.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNAPETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFS"
     gene            685..1116
                     /gene="tnpA"
                     /locus_tag="ACHLIF_003807"
     CDS             685..1116
                     /gene="tnpA"
                     /locus_tag="ACHLIF_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO9607736.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(1132..1320)
                     /locus_tag="ACHLIF_003808"
     CDS             complement(1132..1320)
                     /locus_tag="ACHLIF_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO9607737.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(1324..1683)
                     /gene="yeaR"
                     /locus_tag="ACHLIF_003809"
     CDS             complement(1324..1683)
                     /gene="yeaR"
                     /locus_tag="ACHLIF_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO9607738.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1856..2494)
                     /gene="leuE"
                     /locus_tag="ACHLIF_003810"
     CDS             complement(1856..2494)
                     /gene="leuE"
                     /locus_tag="ACHLIF_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO9607739.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(2621..3544)
                     /gene="dmlR"
                     /locus_tag="ACHLIF_003811"
     CDS             complement(2621..3544)
                     /gene="dmlR"
                     /locus_tag="ACHLIF_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO9607740.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3647..4732
                     /gene="dmlA"
                     /locus_tag="ACHLIF_003812"
     CDS             3647..4732
                     /gene="dmlA"
                     /locus_tag="ACHLIF_003812"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO9607741.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4983..6593
                     /gene="yeaV"
                     /locus_tag="ACHLIF_003813"
     CDS             4983..6593
                     /gene="yeaV"
                     /locus_tag="ACHLIF_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO9607742.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6625..7749
                     /gene="yeaW"
                     /locus_tag="ACHLIF_003814"
     CDS             6625..7749
                     /gene="yeaW"
                     /locus_tag="ACHLIF_003814"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO9607743.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSIELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7805..8770
                     /gene="yeaX"
                     /locus_tag="ACHLIF_003815"
     CDS             7805..8770
                     /gene="yeaX"
                     /locus_tag="ACHLIF_003815"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO9607744.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8824..9939)
                     /gene="rnd"
                     /locus_tag="ACHLIF_003816"
     CDS             complement(8824..9939)
                     /gene="rnd"
                     /locus_tag="ACHLIF_003816"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO9607745.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPALISGWRGELMAEALHNLLQEYPQ"
     gene            complement(10021..11706)
                     /gene="fadD"
                     /locus_tag="ACHLIF_003817"
     CDS             complement(10021..11706)
                     /gene="fadD"
                     /locus_tag="ACHLIF_003817"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO9607746.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11911..12492)
                     /gene="yeaY"
                     /locus_tag="ACHLIF_003818"
     CDS             complement(11911..12492)
                     /gene="yeaY"
                     /locus_tag="ACHLIF_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO9607747.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(12532..13227)
                     /gene="tsaB"
                     /locus_tag="ACHLIF_003819"
     CDS             complement(12532..13227)
                     /gene="tsaB"
                     /locus_tag="ACHLIF_003819"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO9607748.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALVYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(13285..15195)
                     /gene="yoaA"
                     /locus_tag="ACHLIF_003820"
     CDS             complement(13285..15195)
                     /gene="yoaA"
                     /locus_tag="ACHLIF_003820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO9607749.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            15327..15671
                     /gene="yoaB"
                     /locus_tag="ACHLIF_003821"
     CDS             15327..15671
                     /gene="yoaB"
                     /locus_tag="ACHLIF_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO9607750.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3833 a         4168 c         3891 g         3909 t
ORIGIN      
        1 ttgctgaatt tgaccttgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
       61 ttctgaaaac tgtttacagg cccaaagact gtgccatctg acagcgtggc gagtttagcc
      121 acgccagcat ccagcccgat cattgatgct gaagggtgaa ccggagttga tacttcactt
      181 tctgtctgaa tactgatgta ccacttaccg caggactggc tgaccgtgac atttttcaca
      241 acacccgtga cctgacggct attccggtag cgcatccagc ccagtttcgg cagaaaaata
      301 cggctgtttt cctgatcgag ctttacgccc tgcgggtagc ggaatgcatc attctgtccc
      361 cgctttttga atcggggaaa agcagcccgc ttccggaaga agtttttgta agcccgctca
      421 aggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca ttgcgtttca
      481 ggggcgtttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
      541 ccggcctcat gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg
      601 cctgcgaagc gcctcatttc acactcctgt tgaccacctg gtcttaactg gaatttaaat
      661 gcctgtaatc gtttcacagg tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc
      721 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccagatac
      781 cgactccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
      841 tgtgctgatt ttgaagctga acttgttgaa atggatggcg aaccagatca cgtccatttg
      901 ttaattaact atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta
      961 tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
     1021 gttttgtgga gtcctggcta ttttgccagt agctgcggag gtgcgccaat atccgtcatc
     1081 cgccaataca ttgaacaaca gcaaacacct ggttagatgg aaaaccgcgc cttatatccc
     1141 cgccctgaag gacggggttt tacggcgcac cggataagaa cgcacggtat tcaccagatc
     1201 ttttatcact tcagccgcca cttctggcac cagcaaagtc atcggcgtct ctgtttcata
     1261 atcgacagaa acgccattgc tgttattggt gacggtcacg gtatacgttg ctttgcccat
     1321 gattcatttc ccgttatgaa tgactttccg ttgttgcgca ccttccatca ggacttcagg
     1381 agccacgaag aagtcaatgt tgaaataagt atcgtcagtc atggcttcaa tgttgtgcca
     1441 cttttctgga gggaacaccg caaactgccc cgcttcgata aggatcacct gatcaggctc
     1501 tgcactgtgt tcatcagcgt agccgagata tttgaccgcc ccatgcataa cggaaaggcg
     1561 tgggtaaacc cccgggcgcg ttcctttatc aagatgacgt tcgaatattc cggcaggcgc
     1621 agtttgttta ttccagaaag gcgttgagcg cgtatgaata taattctgtg ggatttgcag
     1681 catcctattc cctccttcgg ggaatgcgct gaaaacggtt tattccagcc gtttcagggt
     1741 acgcctgata atttgcattt taaataccat ttattggtta ctttttagca ccatatcaga
     1801 gaaaaatcag ggaggattac aagtggggag cccgcgacaa cgcgggctga atgtatcagg
     1861 attgcagcgt cgccagtcgg gcggcgaaac ccacgaacat caaaccaatc agtgagttgc
     1921 caactttagc cagtttcttt ttggtacgta tgtactgcgt gacaaaagca ccagaaataa
     1981 tcaggaagct caaatagcag aaactcacca gttccagcgt cgtcgccaga ataaagaatg
     2041 aaattcccgt atgtggggca ttaacatcga taaactgtac gaaaaacgac acatagaaca
     2101 aaatggcttt cggattagtc aggctcaaaa ttaacgcgcg tttaaaaatg gccccgtatt
     2161 ggggctcatc ggatttggcc tcgttatttt tacccttcag ggtcgcgtag agaattttac
     2221 tccccagata gagcaaataa aacgcaccaa gataacgtac aatgttgaat aatatcgggg
     2281 tggtcttaat taatgtcgcc actccagccc atgccagaaa catcaatact gcatcgccaa
     2341 taaatacacc gcaggccgca agataaccgc ctttcatacc gctactgacg ctatttttga
     2401 gtacaaacag ggtatttggc cctggcacca acacaataaa aatggcccca accagatagg
     2461 tccagtaatt cagaaccccg tattcagcga acacattaac ctctttaatt atctttcgat
     2521 catgcgcgat taaaggtgaa tatgctaacc aatctgtagc ggcttagaaa ggagaaaatc
     2581 aggttttaac ccgataccaa cccgataatt gaatcattaa tcaggcatgc tccagtgaaa
     2641 aattcgggta gtgctcggca aaatactggc gtaaaaattc taccgttatc cgcactttcg
     2701 ctgacgtcgc cagccttgaa acataaacgg cccagacgtt cgctggctgg taatattccg
     2761 gtagcacttg cactaaatga ccactggcaa tgttttcgct aacatcccac caggagcgca
     2821 gagcaatccc ctgcccatcg agacaccact gatggacaat ttctccatga ttagacgaca
     2881 gtgggccggt gactttaatc gcatggtggc cttctttatt gcgtaattgc cagacaccaa
     2941 aaggatgatc gcgctcttta atgaccaggc acggcaatgc ggaaagatcg gttaaatgtt
     3001 tcggcgcgcc atgttgcgca ataaattccg gcgacgcgca gagaatgcgg taattagtcg
     3061 ccagtttgcg ggcgattaaa ttaggggcga tgtcatcgcc aatgcgaata tcgagatcaa
     3121 caccttcatt gaccaaatcg accaaccggt cttccacatc aaaacgtaat tccagttgcg
     3181 gatacgcctt agccagcgct gataacgccg gagccaccac ctgtcgtcca aaaccaaagc
     3241 tgctgataat acgcagcatc ccctgcggca cctgacgcac gtcagaaagt tcgtccatca
     3301 tctgaccgac atcctgcaaa atccgctgcg cccattcata aatccgctct ccttcttcgg
     3361 taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt tgctccagca
     3421 aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc acggcggcaa
     3481 aaccagcccg acgagccacc agcataaaga cgcgcaaatc attcagcagc ggtaaattat
     3541 tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc agtcaattat
     3601 aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga tgaaaacgat
     3661 gcgtattgct gcgatcccgg gagacgggat tggcaaagaa gtccttcctg aagggattcg
     3721 cgtgttacag gctgccgctg agcgctgggg cttcgccttg agttttgagc aaatggagtg
     3781 ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact ggcatgagca
     3841 acttagccgt ttcgacgcca tctattttgg tgccgtcggc tggccggaca ccgttcctga
     3901 ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc agtacgtcaa
     3961 cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa aacagcctgg
     4021 cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct cgctcggcgg
     4081 tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat ttacgcgtcg
     4141 tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc cacgtaagac
     4201 gctaacttct gccactaaat caaacggttt agccatcagc atgccgtact gggatgagcg
     4261 agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc atattgatat
     4321 tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg cgtccaattt
     4381 atttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg gcattgcccc
     4441 atccgccaac ctgaatccgg aacgcacttt cccgtcgctc ttcgagccag tccacggttc
     4501 cgcgccggat atctacggga aaaacattgc taaccctatc gccacgattt gggccggtgc
     4561 aatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc ataacggtat
     4621 tctggcagca attgaagaag tgattgctca cgggccgaaa acacctgata tgaaaggcaa
     4681 tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt aaggtcaaac
     4741 cagtttattt gaaccgcgtc actgacgcgg tttttttatt cgttctttgc agtaaataac
     4801 ctgcgtcatt tcacctttta ttgtttccgt ttcgtgtttt atggctttcc gtattcttaa
     4861 ttgtttaatt tatgtaacat gcaaataatc aatttgttaa acacaacata caactcatta
     4921 ccctgcaaat aattacaact ggcaaaagcc agagacgggc ttttatgccc aggagatgac
     4981 tcatgatgag caatgttaag aaaaaagatg tgccgctgat aagcatcagc ctggtggcca
     5041 ttcttttcat cgcagctgca ttaagccttt tcccacaaca atcggccgac gcggccaacg
     5101 caatatacac ttttgttacg cgtacgttag gttccgccgt acaggtattg gttttgctgg
     5161 caatgggact ggtgatttat ttagccacca gtaaatacgg caatattcgt cttggcgaag
     5221 gaaaaccgga atacagcacg ctctcctggc tgtttatgtt tatttgtgcc ggtttaggtt
     5281 cttctacgct ttattggggg gttgctgaat gggcctatta ttatcaaacg cctggattaa
     5341 atatcgcacc gcgttcacaa caggcactcg aatttagcgt cccttactcc ttcttccact
     5401 ggggcatcag cgcctgggca acttatacgc tggcctcatt aatcatggct tatcactttc
     5461 atgtgcgtaa aaacaaaggt ctgagtcttt ccggcattat tgccgcgatt accggcgttc
     5521 gcccgcaagg cccatgggga aaactggttg atttgatgtt cctgatcgcc actgtcggcg
     5581 cactgactat ttcccttgtt gtcaccgcag caacctttac tcgtggactt tccgcgctga
     5641 ccggtttacc cgataacttc accgtgcagg catttgtgat cctgctttcc ggcggcattt
     5701 tttgcctaag ctcgtggatt ggtatcaaca acggtttgca acgtctgagc aaaatggttg
     5761 gctggggcgc gttcctgctg ccattactgg tgctgattgt cggcccaacc gaatttatta
     5821 ccaacagcat catcaatgcc atcggtctga ccacgcaaaa cttcctgcaa atgagcttat
     5881 tcaccgatcc gcttggcgat ggttcattta cccgcaactg gaccgttttc tactggctgt
     5941 ggtggatctc atacacccct ggcgtagcaa tgtttgtcac ccgcgtttcc cgcggtcgta
     6001 agattaaaga agttatctgg ggactgatcc tcggcagcac cgtcggttgc tggttcttct
     6061 tcggcgtaat ggaaagctat gccattcatc agtttatcaa tggcgtaatc aacgtcccac
     6121 aggtgctgga aacactgggc ggcgaaacag ctgtacagca agttctgatg tcgttgccag
     6181 ccggtaaatt gttcctcgcc gcatacctgg gcgtgatgat tattttcctt gcctcgcata
     6241 tggatgcggt ggcctacacc atggctgcga ccagtacgcg taatctccag gaaggtgacg
     6301 atcctgaccg tgggctgcgt cttttctggt gcgtggtgat cactctgatc ccgctttcca
     6361 tcttgtttac cggtgcttcg ctggaaacga tgaaaaccac cgtcgtgctc acagcccttc
     6421 ccttcctcgt cattttactg gtgaaagtcg gcggatttat tcgctggctg aaacaggatt
     6481 acgccgacat tccggctcat caagttgaac attatctccc gcagacaccg gttgaagccc
     6541 tggaaaaaac gccagtgctc cctgcgggaa ccgtattcaa aggcgacaac tgagcgccat
     6601 catcctaacg ataaaggtat ccctatgagc aatctgagcc ctgactttgt actacccgaa
     6661 aatttttgcg ctaacccgca agaggcgtgg accattcctg cccgttttta taccgatcag
     6721 aacgcgtttg aacacgaaaa agagaacgtc ttcgccaaaa gctggatttg cgtcgctcac
     6781 agcattgaac tggcgaatgc caatgattat gtgacgcgtg agatcattgg cgaaagcatc
     6841 gtgctggtac gcggtcgtga taaggttttg cgcgcgttct ataacgtgtg tccgcaccgt
     6901 ggtcatcagt tgttgagcgg tgaaggaaaa gcaaaaaatg tgattacctg cccgtatcac
     6961 gcatgggcat tcaaactcga tggcaatctg gcccatgcac gtaactgcga aaacgtcgcc
     7021 aatttcgata gcgacaaagc gcaactggtt ccggtgcgtc tggaagaata tgccggattc
     7081 gtcttcatca acatggaccc caacgccacc agcgtagaag atcagttacc cggtctgggc
     7141 gcgaaagtgc tggaagcctg cccggaagtc cacgatctga aactggcggc ccgctttacc
     7201 acccgcacgc ctgccaactg gaagaacatt gtcgataact atctcgagtg ctatcactgt
     7261 ggtccggcgc atccaggttt ctccgactcc gtacaggttg atcgttactg gcacaccatg
     7321 cacggtaact ggacgctgca atacggtttc gccaaaccgt ccgaacagtc gtttaaattt
     7381 gaagagggta cggatgcggc attccacggt ttctggctgt ggccgtgcac gatgctgaac
     7441 gtcaccccga tcaaagggat gatgacggtc atttatgaat tcccggtgga ttctgaaact
     7501 accctgcaaa actacgatat ttacttcacc aatgaagagt taaccgacga gcaaaaatcg
     7561 ctgattgagt ggtatcgcga tgtgttccgt ccggaagatt tacgtctggt tgaaagcgta
     7621 cagaaagggc tgaaatcgcg tggctatcgt ggtcaggggc gcatcatggc cgacagtagc
     7681 ggtagtggca tttccgaaca tggtatcgcc catttccata atctgctggc gcaggtgttt
     7741 aaggactaat gacatcggcg gcggtatttt ccgccgctgg gctgattttt gatggagtac
     7801 agcaatgtca gactatcaaa tgtttgaagt acaggtgagt caggtggaac cgcttaccga
     7861 acaggtgaaa cgcttcacgc tggtggcaac cgatggcaaa ccgctgcctg cgttcaccgg
     7921 aggaagtcac atcattgtgc agatgagcga tggtgataac cagtacagca atgcgtattc
     7981 actactgagt tcgccgcatg acacctcttg ttatcagatt gccgttcggc tggaggaaaa
     8041 ctcgcgcggc ggttcccgct ttttgcatca gcaggtaaaa gtgggcgatc ggttaacgat
     8101 ttcaacgcct aataacctgt ttgcgctaat tccctcagcc agaaagcatc tgtttatcgc
     8161 gggcggtatt ggtatcaccc ctttcctgtc gcacatggca gagctgcaac acagcgacgt
     8221 cgactggcag ctacattact gctcgcgaaa tccagaaagt tgcgcatttc gtgatgagct
     8281 agtccagcat ccgcaggctg agaaagttca cttgcatcac tcatcaaccg gaacacgact
     8341 ggaattagcg cgattattgg cggatatcga acctggcaca cacgtttata cctgtggtcc
     8401 cgaggcgcta aatgaagcgg taagaagtga agctgcgcgt ctggacatcg tcgccgatac
     8461 gctgcacttt gagcaatttg ctatcgaaga caaaaccggc gatgccttta ccctggtgct
     8521 tgcccgttcc ggaaaagagt ttgtggtgcc ggaagagatg actattttgc aggttattga
     8581 aaataataaa gccgcgaaag tggaatgttt atgtcgtgaa ggggtatgcg gaacctgcga
     8641 aacagcaata ctggaaggtg aagctgacca tcgggatcag tattttagcg atgaagagcg
     8701 tgccagccag caaagtatgt tgatctgttg ttcgcgtgcg aagggtaaac gcctggtgtt
     8761 ggatttgtag tttgccaaag ccggatgtgg cgctgagcgc gcccagtccg gcttcggaag
     8821 attttactgc ggatattcct gcaataaatt gtgtaatgct tccgccatca gctcaccacg
     8881 ccagccggaa atcagcgccg gcaaattgtt ctgcggtttc agtttccagt gccagttcag
     8941 cagttggttg atttgccgac gcgatgccag caattcggcg ctgatcttat gcgtttcgct
     9001 cacgtcagta atcagcgact taatcgcttt aaacacttta cgataaccag gcatgtccat
     9061 caggttaagc atcggctgcg gtaaggcctc ttccggcaat gtctgcgctt tttccaccag
     9121 cgccagcagc gttttaccgt gaaagcggat ttcgctaccg gataaaccca ggctgtccag
     9181 ttcgcctaaa cttcccggca tataacgcgc gaccgaccac aaatgctctt cacgcacgac
     9241 aaagttcacc gccagatcac gctctcgcgc cttgcgcagt cgccagtcgg ctaacagttg
     9301 cagacaggcc agttggcgtg tgcgtaactg ccaggcattg gtgatatcac gccaggcatc
     9361 ttccggcgca acgacttcct gacgacgcat ttgcatcagg cggcattcat ccagcgccgc
     9421 aggtagccag ccggaggcct ccgtttctac cataagcttg gcggtgatcg gtaacagata
     9481 ccagacatcc gccgctgcgt attcacactg acgttcggtc agcggtctgg ccagccagtc
     9541 ggtgcgcgat tcactcttgt ccagcgtaac gccggaatac tcttccacca tggaagcgaa
     9601 accccatgac atcgggcgtc cgcagaaggc agcaaggatt tgcgtgtcaa tcaagggttg
     9661 tggtaattcg ccaaagacat tgaggaacac ttccagatct tcactgcctg catggagaaa
     9721 ttttgtgatg gacggatcgc gcaggatcgc tttcagcggt gaccagtcgg tgatcccgag
     9781 aggatcaatt agcgccagat gctcgccatc gaaaagttga atcaacccca gctgcgggta
     9841 ataagtgcgc gtacgaacaa attcagtatc cagggctatc gccggaaagg cacggacggc
     9901 ttcacacaaa gaagccagcg catcgtccgt ggtaatcatt tggtaattca aattgttttc
     9961 tctttagtgg gcgtcaaaaa aaacgccgga ttaaccggcg tctgacgact gacttaacac
    10021 tcaggcttta ttgtccactt tgccgcgcgc ttcgtcacgt aattctcgtc gcaaaatttt
    10081 tccgacgtta gatttcggta actcatcacg aaactccacc agcttcggta ctttgtagcc
    10141 cgtgagctga cggcggcaaa aggtcaccag tgactcttcg gtaagcgatg gatctttttt
    10201 cactacgaag attttcaccg cttcaccact ggagccggaa ggtacgccaa cagccgcgac
    10261 ttcctgtacg ccaggatgct gcatgacgac atcttcaatc tcgttgggat agacgttaaa
    10321 accggaaacc agaatcatgt cttttttacg atcgacaatg cgcaggaacc cttcttcatc
    10381 catcaccgcg atgtcgccgg tgtgtaacca gccatttttg atgatctcat ctgtagcatc
    10441 cggacgctgc cagtaaccca gcatcacctg cggtcctttg acacaaagct cacccggttg
    10501 acccggtggt acttcattat catcatcatc caccagtttg gcttccgtcg acggcaccgg
    10561 caaaccgatg ctaccactat gataatcaat atcatatggg ttaacgctga ccagcggcgc
    10621 acactcggta aggccatagc cttccagcag atactgtcct gtcagtttca cccaacgctc
    10681 tgccaccact tgctgcactg gcatccctcc gcctgcggaa agatgcagac tggagaaatc
    10741 cagctgctgg aactctttat tgttcagcaa cgcattgaac aaggtgttaa cgcccgtgat
    10801 agcggtaaac ggatatttcg ctaactcttt taccaaccct ggaatatcgc gcgggttagt
    10861 gataagcagg ttctgcccac ccagttcgat aaacagcagg cagttaatgg tcagggcaaa
    10921 aatgtgatac agcggcagcg ccgtcaccac cagctctttg cccggatgca acagcggacc
    10981 ataggtcgcg ttaacctgtt ccaggttcgc cagcatattg cggtgagtca gcatcgcgcc
    11041 tttcgccaca ccagtggtgc cgccggtgta ttgcagaaaa gctaaatctt ccggcaccag
    11101 ttcgggtttg acgtactgca tccggtagcc gttatgcagt gcgctacgaa atgaaatggc
    11161 atctggcaga tggtatttcg gcaccaaacg cttgatgtat ttaacaacga aattgactac
    11221 cgtgcctttt gccgtagata gctgatcgcc catacgggtc agaattacgt gctgaacggc
    11281 ggttttatca accacttttt ccagtgtgtg agcaaagtta gacacgataa caatcgccga
    11341 tgcgccgcta tcgttaagct gatgctcaag ctcacgcggg gtatacaacg ggttaacgtt
    11401 tacgacgatc atcccggcac gcaaaatgcc aaacagcgcc accggatatt gcaataaatt
    11461 aggcatcatc aacgcaacgc gatcgccttt cttcagcccc aacccttgtt gcaaataagc
    11521 ggcaaacgcg cgactgcgtt cttccagctt gcggaaggtc attacctccc ccatattcac
    11581 aaacgcaggt tgatcggcgt agcgcgcgac cgactgctca aacatatcta ccagagattg
    11641 ataacggtca gggttgatct ccgtcggaac gtccgcggga taacggttaa gccaaacctt
    11701 cttcaattct tcacctctaa aatgcgtgtt cgtcgtcatc gcaaccccaa atgatataca
    11761 tgccgttaac ataatattaa ctcatcatac cagcttgata attacccaac gaaaaggttg
    11821 cgaagcgcgt cactatttat ttttatcttt accgtaagaa tgcagaaaca gcggaccagc
    11881 cgctgtttct ttttctttaa aaacaagcaa ttattcagtt acaactgttt gtactctcgc
    11941 ggggccggga ttataccagc cccatccgcc gtagccatag ggccagccac gaccgccata
    12001 aaaccatgga tcaatcggct gaggcggcat aatcacctgc tgggttaaat gccaacgttt
    12061 gtaacccgtt acttgcatca ccataaattt atagggcgta ttgccgattt tgccgtcaac
    12121 cgcaccagtg attggcccga ctaccgtaac aagttgtcca cggaaatcca ccgggtccag
    12181 aaaaccgttc acatcggcat aaatgcgacc gcgagaaggt tctcccagcg tcggtctggc
    12241 tccgctgtcc agcggtacgg tagcaatttc caggcgggtt ttcccttgct ggttttgtac
    12301 cgcaaccact ttgccaccaa agcgtgcctc ctgaccaacg tacagctgcg gcgcactcat
    12361 cacccgaact aaatcttgtt gcggcgtggg actgctgcct ttaatggcgt ccggcacagt
    12421 gacacaaccg ctcagcatta gcgcaaacgt acctgccagt atgcctttga taacattctt
    12481 ttgaaccgcc atgatgcgac tccttttttt ctcagggcat actcttaaga ttcattcttt
    12541 gcccggaagt ttcttccatg cgacgttgtt acgtaaataa accggttcgg catgttccac
    12601 cgccaccgtt ttaccctcgg caaacatctg acacgcaatc ggcagcatat cttccgccgc
    12661 tggcagtaaa acttctccgt cgcgcaaaac cagcccgctc tctttaccga gatccggcca
    12721 ggcttgccag cccgtcccta ccgttaccca ttcgccggaa agctgttgca ttcgttcatg
    12781 aacgagttcg ggtttgagta cggcttcggt ttcttcaccg cgccagatac cgttttcatc
    12841 acgctgatat tcggcccagt aaacttcgcc cattcgcgcg tcaatggctg ccagcacgcg
    12901 ggttgcgccg tttttgcgcc acgccccttg cgccatcgtc attagcgtgg agacgccaat
    12961 catcggtagc tctgcgccaa gtgccagccc ttgcgcaatg ccaataccaa tgcgcacgcc
    13021 ggtaaagcta ccggggccgc gcccgtaaac cagagcgtta atatcagtca gggaagttcc
    13081 gctggtggtc aggatatcct gcaccatcgg taagattcgt tgagtatgtt cacgagggca
    13141 aagctcaaaa tgagcgttga cagtaccgtc gttccacagg gcgacagagc aggcctctgt
    13201 cgcggtatcg atagccagaa ttcgcatggg tcttcgtgct tagatcaata aaaaggcgcg
    13261 catcatacca tactccgtaa caaattacct ggaggatggt atcgcaagga aacgaaccgc
    13321 acgggcaatg tcacgagtgc gtggcgcggg cggcagactg gcgagaaacg tcgcgccgta
    13381 aggacgcatc accagccgat tgtcacaaat caccagcacg ccacgatcgt cggcgtcgcg
    13441 aatcagtcgc cctaccccct gtttgagagt aatgacggca tctggtagtt gcacttcatc
    13501 gaacgggtcg ccaccgcgca aacgacaatc ttccatgcgc gcttttaaca gtggatcatc
    13561 cggcgaggta aacggcaact tgtcgataat caccaatgac aatgtatcgc cacgcacgtc
    13621 caccccttcc cagaaactac tggttgccac aagaagcgcg ttaccggcgc tgacaaattg
    13681 ctgcaacagt tgccctttgc tggtttcccc ctgcaacaaa acgggaagcg tcatggtagc
    13741 gcggaactgc tcggccagat cacgcatcat ggcgtgcgag gtacaaagca taaaacaacg
    13801 accgttgtta gcttcgatga tcggtcgcag cattgccgct aactggcgag cagaacctgg
    13861 ctggttggtt tgcggcagat tgcgcggcac acagagtaac gcctggcggc tgtaatcaaa
    13921 cgggcttggc aatagcaacg actccgcctg ttctatccca agccgcgagg tgaaatgatg
    13981 cagatcgtcg ttcaccgaca gcgttgctga ggtgaagatc cagctaccag gcttttgcgc
    14041 cattaactct ttgaatttat ccgccacgct gagcggcgtg agagccagag taaaatggcg
    14101 cgaagtgcat tcgtaccagt agctgtagcc cggctgattg atctctttta gccgcttcag
    14161 ccgtgtgcga tacaacgtag cgcgctcaaa tgccgcatcc agcaaggcgg aacgccccag
    14221 tgacagtttc gccacgtcat aacaaagttc cagggtgtca tcgagcagta aaaatgcccg
    14281 ctgaatttgc ggattagcta acagctcacg caggttaccg cgataacctg gctcaccgag
    14341 ttgcagacga aaatcctgcg cactctgggc aagacgatca gcgcactttt gtaactgctg
    14401 ggtgtctttt aattcggtgc ggtaagcgat ggtgatgtct tttgccaggt cgagcagttg
    14461 tcgactggag agtgactgac caaaatactg gctggcaatg tccggtagct ggtgggcttc
    14521 gtcgaagatc atgacgtccg cttccgggat cagttcgcca aatccactct ctttaaccac
    14581 catatccgcc agaaagagat gatggtttac caccaccaca tcggcgtcca tcgctttttt
    14641 acgtgctttg accacaaagc aatctttata catcgggcag tcgctgccaa gacagttgtc
    14701 gttggtgctg gtgaccagcg gccacgcctg tgaatcttcc gccacgctga cgcaggtgct
    14761 gatatcacca tcgactgttt gattagacca ggagcgcagc aggatcacat cgcttaagat
    14821 ttgtaccggc agatcgcccc ccgccagcgc ctgctgttcg agacgctcga ggcagaggta
    14881 gtttgagcgc cctttcagca gcgccacgtt gcccgtatat ttcaatgcct ttgagactgt
    14941 tggcaaatcg cggctgtaga gctgatcctg caacgctttt gagccggtcg agataatgac
    15001 tttctttttc gcccgcagcg caggagccag gtaagcgtag gttttgcccg taccggttcc
    15061 tgcttccacc accagcggct ggcctttttc tatcgcctgg gtgacggcta ccgccatctg
    15121 tcgctgtggt tctcgcggct taaagcctgg tatcgctttc gccagctgac cgtctggtgc
    15181 aaaatcgtcc gtcacactac cccctgttga tttgaacagg gattatgtca ggatgagggc
    15241 gctttcgcca gttgaattgg tgacggcgac ctcacattgt ggcagtcttt gcagcacgaa
    15301 aatggaagga taatgaggac aaaatgatga ctatcgttcg tatcgatgct gaagcccgct
    15361 ggtctgatgt ggtaatccac aacaacacgc tctactacac tggtgtaccg gaaaacctcg
    15421 acgccgatgc ctttgagcaa accgccaaca cgctggcgca gattgatgcc gtgctggaaa
    15481 aacagggcag caataaatcg agcattctgg atgccaccat tttcctggcc gataaaaacg
    15541 acttcgcggc gatgaataaa gcgtgggatg cctgggttgt cgcgggtcat gcgccggtgc
    15601 gctgcacggt acaagcgggt ttgatgaacc cgaagtataa agttgaaatt aagattgtgg
    15661 ctgcggtgta agctttatcg aagcaaaata agtcagacga taatttatcg ataatactgg
    15721 tcggttttac ataaatcgac cagagattaa gatattacct gttgatgctc ctgctaattc
    15781 attatttatt tttataattt c
//
LOCUS       DAZGOB010000115        15796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGOB010000115 DAZGOB010000000
VERSION     DAZGOB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.115"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..291
                     /locus_tag="ACHLIF_003822"
     CDS             <1..291
                     /locus_tag="ACHLIF_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HFO9607751.1"
                     /translation="QYDTDIESRANELWKKAVHTINNTTREERRVCYITLNRPEEEHS
                     DPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY"
     gene            complement(512..3232)
                     /locus_tag="ACHLIF_003823"
     CDS             complement(512..3232)
                     /locus_tag="ACHLIF_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO9607752.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWEPPSPGM"
     gene            complement(3244..3576)
                     /gene="mobA"
                     /locus_tag="ACHLIF_003824"
     CDS             complement(3244..3576)
                     /gene="mobA"
                     /locus_tag="ACHLIF_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO9607753.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            3808..4143
                     /locus_tag="ACHLIF_003825"
     CDS             3808..4143
                     /locus_tag="ACHLIF_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO9607754.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(4229..5077)
                     /locus_tag="ACHLIF_003826"
     CDS             complement(4229..5077)
                     /locus_tag="ACHLIF_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9607755.1"
                     /translation="MNQTLPTADLNTAGTTDIIPSMAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTYTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQELIDIWRKLSW
                     DYRTNNPCCLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNIGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            5427..5622
                     /locus_tag="ACHLIF_003827"
                     /pseudo
     CDS             5427..5622
                     /locus_tag="ACHLIF_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5656..5907
                     /locus_tag="ACHLIF_003828"
     CDS             5656..5907
                     /locus_tag="ACHLIF_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO9607756.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(5938..6183)
                     /locus_tag="ACHLIF_003829"
     CDS             complement(5938..6183)
                     /locus_tag="ACHLIF_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607757.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKKGALEV
                     AREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(6183..7073)
                     /locus_tag="ACHLIF_003830"
     CDS             complement(6183..7073)
                     /locus_tag="ACHLIF_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO9607758.1"
                     /translation="MSKKKNTTTPTPHDAAFWSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEEPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSEAELKNITH"
     gene            complement(7138..7404)
                     /locus_tag="ACHLIF_003831"
     CDS             complement(7138..7404)
                     /locus_tag="ACHLIF_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607759.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENIPLHRHTRVRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERACH"
     gene            complement(7497..7931)
                     /locus_tag="ACHLIF_003832"
     CDS             complement(7497..7931)
                     /locus_tag="ACHLIF_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO9607760.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKSAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(8659..9159)
                     /locus_tag="ACHLIF_003833"
     CDS             complement(8659..9159)
                     /locus_tag="ACHLIF_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO9607761.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPVNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(9621..10218)
                     /locus_tag="ACHLIF_003834"
                     /pseudo
     CDS             complement(9621..10218)
                     /locus_tag="ACHLIF_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10215..10934)
                     /locus_tag="ACHLIF_003835"
     CDS             complement(10215..10934)
                     /locus_tag="ACHLIF_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO9607762.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(10931..11179)
                     /gene="psiB"
                     /locus_tag="ACHLIF_003836"
                     /pseudo
     CDS             complement(10931..11179)
                     /gene="psiB"
                     /locus_tag="ACHLIF_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
     gene            complement(11174..11413)
                     /locus_tag="ACHLIF_003837"
                     /pseudo
     CDS             complement(11174..11413)
                     /locus_tag="ACHLIF_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11458..11944)
                     /locus_tag="ACHLIF_003838"
                     /pseudo
     CDS             complement(11458..11944)
                     /locus_tag="ACHLIF_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(11904..12122)
                     /locus_tag="ACHLIF_003839"
     CDS             complement(11904..12122)
                     /locus_tag="ACHLIF_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607763.1"
                     /translation="MNYSGFEDMREQVAELANTMCDLRTTMNEMEQRYSDADTLPERL
                     VRQTLFRASHLFMKAYNEILELDYCFKD"
     gene            complement(12122..12805)
                     /locus_tag="ACHLIF_003840"
     CDS             complement(12122..12805)
                     /locus_tag="ACHLIF_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO9607764.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAVWIRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNSIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAVN
                     DNRFEPEAA"
     gene            complement(12881..13192)
                     /locus_tag="ACHLIF_003841"
     CDS             complement(12881..13192)
                     /locus_tag="ACHLIF_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607765.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(13189..14091)
                     /locus_tag="ACHLIF_003842"
     CDS             complement(13189..14091)
                     /locus_tag="ACHLIF_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO9607766.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTYLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWYQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSHP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            14364..15323
                     /locus_tag="ACHLIF_003843"
     CDS             14364..15323
                     /locus_tag="ACHLIF_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO9607767.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALMKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            15323..15718
                     /locus_tag="ACHLIF_003844"
     CDS             15323..15718
                     /locus_tag="ACHLIF_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO9607768.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDNLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIG
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
BASE COUNT         3896 a         4373 c         3917 g         3610 t
ORIGIN      
        1 caatatgata cagatatcga atcccgggca aacgaactgt ggaaaaaggc tgtgcacact
       61 atcaataaca caaccaggga agaacgccgg gtatgttata tcacactgaa tcgtccggaa
      121 gaggaacact ctgatcctga agatatacct tcagtgaagg cgattctgaa taccctgctc
      181 cccctggaga tactgttacc ggtcgctgaa aataataaat cgcagacaat aaataatgat
      241 catccccaga aatcatgtgg agcacaacca acaactgacg aaaaatactg aaggaataca
      301 aatattttct ggtgaccata tctgtcaatc agactgtaaa accctgttca ctaagtccgg
      361 ggttttacgg cgcactggat aagggccgca gacacggccc ttaattcaga gttaaccggg
      421 tataaactca attcagtgac tactcccgcc cttattgacg aaggttcctg ccgtttttgg
      481 gctgaatctt tgcaccatca cttatccggg cttacattcc aggtgatggt ggctcccatg
      541 ttttctcctg ccgtaacgta taattatcat catggctcac cgataaacgg gtctgctgtc
      601 tgatatattc ccggtaatac tgctcactgc gttgacggtg caaatccacg tccggacgag
      661 taatggtgaa gtgtccgttt ccggttattc ccgctgcatt gtgccaggcc accatttctg
      721 caaacacatc attaaactgc tggtatgaac cttccggctc aaaaccaagc tttccgttgc
      781 ggctgaacag aactggcgca attaaatcaa gcttctctgc cagttcattt cggtccgtgt
      841 gatggtagaa cacaacccga tctccgtaat ccgtacagac attttttcct gtccgcgtat
      901 ccctgaaatg aacacttcca tttttctgaa gacgagcttc aagttttgca acattattaa
      961 aaagtggcgt tcctccgggt tcgcagagta cgacacaacg atcaacatta gttgtgcgtc
     1021 ttttatcctt attacgcgag cgacgatcac gataatccca gccacgtaac tgcgatactg
     1081 cagccctgtc tccctgagcc gcctgttgtt ccacccactg ccggtaggac aaaggatacc
     1141 acttaccctc cgcaatcagt gacaaccgct cttccttcac actctccttc agcctgatca
     1201 gcgcctgcat acgctgcact tccgcaatat ggtaatgcag cttacgcaat tgtggatccc
     1261 ggaactgcac acggatatac gccttgcgcc gacggcaggc agcatgaatt tcccgtaacc
     1321 gttctccgta acgtaagtcg ggtttacgcc agtgctcctt ccatgcaaga taacgcgcac
     1381 gcaaatcttc ccgcgcagct gcacgggctt cacggcgctc acgacgaatc tcaggatcgc
     1441 gccggaagcc tggttccact tcatcactgg cagcaagttc cggattgtaa cgacattcag
     1501 gcttcacccg ttcgaaaata tctgcagccg caatttcaaa tggcccggcc tgaggctcgg
     1561 cacgggataa cgtcaaatca ggatggatac tgctggcctt caccggtgtc agctcatgat
     1621 taaacgcatc cacaatcaca agaccatgat gctgtttctg cagcataagc cctttacgcg
     1681 caaacatctc atgacatgcc tgccagctga aatccccgtc agctatcatc tcttcaatca
     1741 ggctcctgca ctgaataagg ccgtatcccc acagactttc cttatcagaa aacagttcgc
     1801 gctttattgc tccctgtgga cctgtcccca tccgcatacg ataccattcg taatcactga
     1861 cagcctgagt accaagatta cgtccccgga accgttctcc gggtataacc tgagtaaaaa
     1921 tatccttcgg tacaggctgc catccaccat ccagatcctg catataatcc agcgtcatta
     1981 acggccacac actgtctgca cgtactggtg tctgttgttt atcaaatcca tcatgcagta
     2041 ccagatgccc gtgctggatc cccatccata atccagccgt tgccagagtt ctgtgcacat
     2101 ccaggcaact ttccagctca ccatttctcg ccatttccgg taagcgttcg gcaaaatgac
     2161 ggcaggcgta ctgtttcaga ctctccgttt ctgccacttt ttccggccgg atatcaacgt
     2221 ttatggcttc cggatcataa cgaccgggtg tccccacctg actgaaaata tctgtcggta
     2281 caggctgata ttcccccagt ttccgcccca gtttttcagc tgtccataac ggtccgaacg
     2341 aactgagtgc aacaccttcc cgcgctctgt cccagccatc cttcaccacc agttcccctt
     2401 cctgcatcgt gatatacaga ccatcactgg ccagccgttt atgcaaagat aaccagtcct
     2461 gtgcgggctc ttcacgcagc ccggcaatcg acatctgaac aatatattcc ctgaacgtct
     2521 gctttttccc gcgtcgccag gcattacggc gctcacgtac cagcgctgtt ttgcgcacaa
     2581 tacgattgcc cggtgcatgc acaaagcagc cattatccgg cgcgaatccg tgcttaattt
     2641 caagttcacg gcatgcacga ctgagttttt cctggctcca cggaaggcaa ttgatatagc
     2701 cggtttcagg gtggacacga ttcactgcaa catgtacatg caggttatcc gtgtctgtat
     2761 gaacagctgc cacatactga tgttttgaca gctcaaggga tttcagggtg tgacgcacac
     2821 agtcaaaaag ctgctcagaa cgcggacttt cgtgcgcagg ccaggagagt atatagtgaa
     2881 aaacaggatc tgtatcatct ttggaaaaat gagcaaggtc tgctgctttc tgcatttctt
     2941 cagccgcaga ttcaagagac aaacagttat gaaagcaggt cacaccatag aaattcaccc
     3001 actgactgcc gtctttcatc acatcaatca gggaaacaaa tttttcatta cgcagacgtg
     3061 ttgcataatc aacaagacgt ctgaagcggt tccggtgcgg aatctcagct ttcatttccg
     3121 tgtctggcct gagttcatct tccggcacat catcccgcac tgaggtgtac gcaatcaggt
     3181 cttcaaaaga agactttccg tcgcgtcttt ttttcggaat gatggcattc atcagcgatt
     3241 atctcagcgt cccattaaat ctcttcgcag agtattagca agctctgtaa tagcaacgag
     3301 aacctctgca tactctttat ctcccgtgcg ttttccttcc acaaacagat gtttctgcag
     3361 gcgaccaaga cgcatcagct ctttcaaaaa atcttcgtca atgcgggcat ttatctgttt
     3421 tcccagcaca gtttttctta tcagagttga aactgtcagt ccgttttttt ctgccttttc
     3481 tttaacaatc tcagcctctt ctggtgaaaa acgaacagca atgaggacat tcttttgccg
     3541 tttctcactg ccacttcggg ttttcttctc actcatggtc tgctccttac cggggtgtcg
     3601 gggctaagcc ctgaccaggt ggtaatcgta tagccgtgcg tgcgcggtta tacgattaca
     3661 catcctgtcc cgtttttcag gcattatagc ctgttatttt tgtgcatcat agtcttatgc
     3721 agacggcaga ggcggggcgc agctctgcca ggaggcagtt tttgatgata aaatcagact
     3781 tcaacatgaa acggagaaaa agtgccaatg gcttctaaaa aattttactc agatgatgac
     3841 atccagctgg caaaagctgc tttatcagaa ttgccggacc tcaccgctca acgaaaaaca
     3901 ttgcgtgatt ttcttgatgc cattcgtgat gacatcattg tcctggtaag gacaaagggt
     3961 tatacactgg cagatgttcg tgacacgctc aaaaatgcag ggtatgaagt aggagagaag
     4021 gcacttcgtg acattattcg ggaagcagaa tcgaaaaaac catcccgccg ttcttcctct
     4081 aaaacagcac ctaaaaagac agattcaggc aggaaagatg gcatcgatat gaacaacaat
     4141 tgatgtgaca atgtcacatc gtattctgag attatgatga ttacagaaaa aaaagccccg
     4201 gcataaatac cagggctttt ctgtttaatc agactcgtgg cggcaataca ccaggataat
     4261 gccgtgcaat aatatcattc agtttttcaa caaggtcagg acgcttaaag acaatgtgta
     4321 cggtcccttt ctggtagcca cgcaccgtaa aatattcccc ttcaaacaac tcaccgatat
     4381 tgtcgccgtt cccgagaaat tcaccatact gcgccccgat gctgttcctg aagtcaggaa
     4441 cgttgcgtcc ctccagcaga taaaacggac gtaccaggtc atccagtgcc tcccgtccgc
     4501 tgcagtccag cgtcacacgc ccgttactcc agcggtacag caggttttca ataatgattc
     4561 tcttgcccag acagcaggga ttattggttc tgtaatccca tgacaatttc cgccagatat
     4621 caatcagttc ctgttcaaat gtctgcatct tactggcatt cagatggcgg aatgttgcca
     4681 gtacattgtc cagggtgatt tcaggacagg tgtcgctcat cagctggctg ttccactcat
     4741 cacgctggca tgaactcatg aaggtgtaca tgcccgtata agtcatcagg cgatcccaga
     4801 ttttccggtc ggcaagacgg gtgatattct tttttgcttc ttccggttta tccatgcatc
     4861 gaacggagtc agtcacgcag tcttcaaacc cgtagagaaa aatgtcaccg gaagcatcga
     4921 gcagaatcct gcgcgctgtc gccaggcatt ccatcgcctg catgaacagt gcaatacctt
     4981 cgttacgctg cgcgatgatg cggtcgatag ccatagacgg aataatatct gtcgtgccgg
     5041 cagtattcag gtcagcagtg ggtaaagtct gattcatcat attgtctcct catcgtcacg
     5101 gctgccgggt cagcctggtc atttcccgga ggagcgaagc tccttctgcc gtccacttca
     5161 cagggggttc ttcccgcagt ggacctttcc gatgggggac gctcaccacg tcacggggca
     5221 gttgtccggg ggcagacgca aaagtttttg acgcctgcgg caaaaagttt tgtggctggt
     5281 acatctctcc cctggacgac tgaaacggga tgtggtcgtg tatgtacccc cggaaagtcc
     5341 gtctgcggag aacgggccgc agtgcggcgg cagacaggca gagacagctg cgccatcagg
     5401 cgcagtggcc ttacacaccg tcaggtgtgc agggagcgcc gcatccggcg cgactgaaga
     5461 aaaacgacag cacaacaatg gcatgtggtg gtcactggcg caccactccc gcacaggtga
     5521 aaacagagaa aacagcacga cacctacaac taaaaagtga aatttcttca ctttttcact
     5581 cttatttatc gcgctgacct ggtgattatg atgataaaat aaagtgaagt tttttcactt
     5641 tttcaaaggt gttttatgac cggctgggaa ctgcgaatct ggcgaaaaag catgctctgg
     5701 tcacgcgaaa aagcggccag agaatttggc gtgacacaga gaacatggca cgcctgggaa
     5761 aatgcggaac aggtggatgt caccgtctgg aggaccaccc aggcactttc agtccgtgat
     5821 ctgctgcctc acatgcaggg gatgcgcaag gcagatatca tcaggcgtct ggaaaatgaa
     5881 ctgggggaaa cggcagggaa tgtgtgagcg tgattcacag cctggcagaa tgccaggcta
     5941 ctgctcatca ggaaacagct ttttcagttc ttcaccggaa agtccggttg cctcctgaat
     6001 ccgttccggc gtccagccca tatcccgcag ccggcaggct gcttcccgcg ccacttcaag
     6061 cgcacctttc ttaaaacctt cgtcgaaacc tttctgtata agacgttctg caatcgtcat
     6121 gctgtttgct ctgctcctgc tgacaaaaaa acccggcatt ttgccgggct ttgtggttct
     6181 catcagtgag tgatgttctt cagttcagct tcggaaagtc cggtagcctg cttaatctgc
     6241 tccggcgtca tccccatttt ctgaagctgg cacgcaatag ccagggcttt ttcaagcgct
     6301 ccttcctggc gacctttttg ctcaagacgt tcagcgatag tcatcagttt atcctcatgc
     6361 tgcggcagac gctcagccag cgcccgcata aattccggtg atacagagtc ccctgcgtta
     6421 acgatgtaat gaatcagaac acgcacctgc tcgtctgaaa tttccaccat gacctgcggg
     6481 agtttgtcca gcagctccat catgtcacga tggcggatat gcttcatcag cagcgtcagt
     6541 gcggccatcc ggcgatggtt catgatttca ttgtcatcaa cgacagtgat atcaaccagc
     6601 ggaaacggct ttgtgtatat tttagccgca agcgccggtt cctcaaaaca gtccagccag
     6661 ttcatgctgt acgggtacgg gctgcgctca ccatgataga acagcaccgg aatcaccagt
     6721 ggcaatgttt tgtgctgctc cagatgtctc tgcatcgccg ccacactgta acgtaacatg
     6781 cggaaaggca tgtacagatt ttcggtgctc tggtgctcga tgagcgtata aacatagccg
     6841 tcttcacccc cggtggtttt cacactccag aggatatcgc tggcgtactg atgcaggtcc
     6901 ggctcaacaa aggtggcggg ttccagcttc agcgtggaca ggtcgcacaa ctgccggtac
     6961 tccgccggaa gatgcagttc cagaaaatct ctggcgacgt cgggattcgc caggaacgac
     7021 cagaacgcgg catcatgcgg cgtgggcgtt gtggtgtttt tcttcttact cacggttcct
     7081 cctggtgaga tgtctgaacg tatcctgccg cctgcacagg cagacggcaa gggttaatca
     7141 gtggcaggcc cgctcaattt caagcgcgcg gagcaggtgg ccccattcgc tgactttcat
     7201 ggcggggata cgtacccgct tctgctcact gcagcgtctg gcgatatgac cgctcatcgc
     7261 caggacgtcc gggttaatct catcctgtgc cagctcctga cgtctgacgc gggtatggcg
     7321 gtgcaggggt atattctccc gcaggcactc ttcacgcaga cgctttacat actcggcggt
     7381 tgcgaagata atggatggtc tcatggcata atctccatag atagtgattg agatgccgga
     7441 cttccgtacg gggaagcgcc ggcggagaaa ggcagggttt atcgccctgc cttttttcat
     7501 ttcggacagc ggcgcagtgc cagcgcgagc gctttcatct tccagccttc cggcgtgcgg
     7561 tacatcacct tccagtagcc gtcgatgaac acataatgga agcgcacctc ttcagtgctt
     7621 tcagtggcaa tagcctccat gaaagaacgg tactcacgcg cctcactgtc catccggaaa
     7681 ccactgtcac gtccgtaata agccgtcaca ccgtccaccg ggttctgaag cgtatgcccg
     7741 gcggatttgt cgcaacgggg accgaggcaa cggatatctc cgtgttcaac cagggcttcg
     7801 gccagctgac cggtggcata gtgggtatgc aggatgcgac cgttccaggt cggctccccg
     7861 tgcaggtggc agtaaacggt acggaacacc ccgtcgcggc aaaaaacgga aatcgtgctg
     7921 ctgactgtca tgaaaacgtc tccttctgct gaatcaggtc gccgggccga ccggttgctc
     7981 ccccggaggg gcgaaagccc cttctgccgt ccacttcttc ggggtttctc cccgcagtgg
     8041 acctttccga tggggcagcg actgccacgg cacggggcag ttgtccgggg gcagacgcaa
     8101 aagtttttga cgcctgcggc aaaaagtttt gtggctggta catcttcccc tggacggctg
     8161 ccccgtgttg tggccgcttc agcccccgga aagtccacag cgggaaacag gccgcagggg
     8221 tggcggcaga agggttgact gcgccatcag gcgcagtggc cttacacacc gtcaggtgtg
     8281 cagggagcgc cgtttccggc gcgacagggg cggcgcgacc ggaacggtcg ctgcaggcct
     8341 ggtttttcag acatcggacc gtagcggcag cccgaaaccg cgccagcggg ttcggcggag
     8401 actggcgggt gcgccagcag ctgcctgtcg gagtcgggcc gacgaccgga tgccgccagg
     8461 cagccgggag ccgaagggtg tccccgcctc cgggcatcag aacaacagaa acgacaaaac
     8521 agaaaaatgc ctcacaccat gacgggcagg aagggcagcg ccgcaggcgc ggccctcctg
     8581 tatttcaggc aggcgtaagc ctgcctgggt tttccggcgg cccggcaggc catacccccg
     8641 ccggagcggg ggagtaaatc accgacggaa cacatgaccg tcaataaagg tgaaggagtc
     8701 caggaataaa tcccgcgcat acgcctcata gtcaaaatag agcgccaccg tctccggcac
     8761 gtcagccagc aggccggtat cactgacgaa ctcaaccgca aaagcctcct cactgtcagc
     8821 ctcgccccac caggcatcgc ggaaggtgtc aaaatccgtc tcaccggtat catccaccca
     8881 gagacgataa gcctcttcgc agccttcatc ccgtgcctgg cggaagcctt caacccaggc
     8941 ccagttgata tggcattcgg aggccatatt caccgggaac ccctcataat cctgaaacat
     9001 cagttcagga tcggcttcat cctggtgaag agcacggcag gcggcgaaaa agtcgcgttc
     9061 atcatcaaac gtggtcaggt caaaccagcg gccagcgatg cttccacagt tgtatttatg
     9121 ccaggttcca acgtatacag caggtgcaac aacagacatt cccgaatcct ccatagtcac
     9181 atgttctgcg acaccgggcg gtgccgtgcc tccatctttg ccgggcctgc agggatgcaa
     9241 gggcgcaagg gaagccgcag cccgacgcgc acggctgacg ggagttggag ggtgagaccg
     9301 cgaaatctga gcgggtgaac gaccggaaaa tcacggcaga cgggaaggag ggaccgggtg
     9361 accgcagcgg ttcatcttac cttgcatccc gcccgcagaa gcccggctac gatggaggca
     9421 cggcacagcc cgcacacgac gctgaggcca cataactgaa aacccacaag gccatgcccc
     9481 gcatggccgg gtaaaagcgg atccgccggc gacttcctta ttccggcgag tgcagaggac
     9541 acgccgcatg acctccagac cacccctgat atgaaacgat catgatcggc gtgtccctgc
     9601 gaaaaatata catgcctggt tcaccggaga atatctgtga tatccacaag ttttctgtcg
     9661 cacagccagt cctgcgccgc acgcaccacg ctactgtccg gtttcaccgc tttcagagaa
     9721 gatgccagtc gtttgatcag catcaccagt tcccgtgtct gtatgtccgg cattgtctct
     9781 ggaagtgcag agggactctc atttccgacg ctcgtaccat gcgcttccag tgtggcacgg
     9841 acaaaccagg cggcatccac cagttgctgg cgaatccagg ccagctcggc atatctgtca
     9901 cgggctgcca ttcgctcacc cgacagcgcc ttgctgatat ggctgcgcac cagattatcc
     9961 aggagttcct gcgcttcttc cggtgaaaag ccacctggta tctctgtgcg caacagtcct
    10021 ttcctgtaca gtagctgcag cagatccaat ctgcgaccgc actgcaacta tcagtacata
    10081 acggacaata ttcgcactgg cggtggtttc tgtatcattc agaagttcct ccggatcatt
    10141 accaccggta ttgcagagca acggcaacag aggtcgcatc cacacgggaa cctgatattg
    10201 ttcgtctgat ttcatcatat tgcgttctcc agcttgttcg ggatcatcca ctgctcaagt
    10261 gcacgtacct gtaccggtgc accacgaccg gcctcaccgg cttcaaaaat cagccgccag
    10321 agtggttcct cctgccagaa aaaccggtca aatgatgaca gtgatggaaa acgcccgcac
    10381 cacccccaga aaatcgtttc cagatcatgc tcctcaacaa caccagacca gcgggacaac
    10441 cagcttccga ccgtggcggg agtatgaaac gcaaggtcaa tctgcgcctg agcaaaacat
    10501 gtctggtatt ccagttcccg tcgttctgcc tcacgttccc gctggcggct gtatttctgc
    10561 tctgtcagcg tggttttctg acgacgacgc tcaccttcac gaaaaggaac ggacgggaac
    10621 agacgatgcc ccgcatcgga tggcagggga agaacacaga cttcaccaaa actttcaatc
    10681 agtttatcca cggcagccag ccacaggaga cggttgccat gaaattcctc cttcgtcagc
    10741 gcaggggcaa aaaagcggag atcggtcagc gaaatccggc gactgccatt caggcagcga
    10801 aagaaagcgc tggcataagg ccatgctgaa agtgtgcgtc cctgacgacg acgttgttct
    10861 gtgacagcca cagcctgcat ggcggcctgt tgttctgcgc tgaggggaat cagtgcccgt
    10921 gaacgtgcgc tcatgcctga cctccttctg ccatcaggat atggatgatg tcgttaacac
    10981 tgtatccctg cgcatcaagt gtcgcggcaa gcgccagcgt gtgactgata gcgacaggcg
    11041 tgaagatatg ttgcacctga acgacggcga aaggctgtcc accacaattc accagaacca
    11101 gcatccagag ctgagagacg tctcccggcg aacataatgc caggtggaaa cgttcgtggg
    11161 ctggcgtgaa accaagttcc gccccggttc cggtcagttt ctgaatggcg gaggccaggt
    11221 gcggcggcag tacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca
    11281 gccgcacgta aatgctgatc cagcccggat acttcaggtt acgctggtca acatccgtct
    11341 cttccggcca gccctgcgcc agcagcaggt cgcgggcatg acgacaaatc agtgcattca
    11401 gcgttttatt catgattcat ggtctccctg aatcgccgcc cggaggcggc gataacatca
    11461 gtcgttttcg tgcgtgttgt ttttcagcag gatgcgggcc tgattaacca ccgcctgacg
    11521 gcgggtaacg cattccggga cggcgaggcc gtaatcacgg gcggcccatt cacgcttcca
    11581 caatgcctgt tcagcggtct gaatcaggga cgccagcagt tcagcaggga cggtcacctg
    11641 gcggttttcg ctcacgttgc tgataatctc catcgccgcg gaggaagaaa taccggactc
    11701 agtccgctgg tcttccggga tgtcctccag gcgcgccagg atggtcctga tttcatgatc
    11761 ggccagggaa atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac
    11821 cagcatcaac ggcgtgtctc ccggatactt tgctgccagc gcccggcaga gcgtttcgca
    11881 tgttccgtac atactctttc ccctcaatct ttgaagcagt aatccagttc cagtatttcg
    11941 ttatatgcct tcataaacag gtggctggca cgaaagagcg tctgacgcac cagacgttcc
    12001 ggcagggtgt ctgcatcgct gtaccgctgc tccatctcat tcatggttgt gcgcaggtcg
    12061 cacatggtgt tggccagttc agccacctgt tcacgcatat cttcaaatcc gctgtaattc
    12121 atcatgctgc ctccggttca aacctgttat catttacggc cccctgctgc atggcccgtt
    12181 gcacggcggc aaggcgttgc tgtccggcac ggtgatactg ttcaagcagc tcgataccaa
    12241 tataccggcg tccggactga agggcggcca cgcaggttga accgctgcct gcaaacgggt
    12301 ccagcacaat tgagttcggg tgtgtgaagc tctcaatcag cggttgcagg ctggtaacgg
    12361 gcttttccgt cgggtgatgg cggttgcccg aatatttcca gcccagcaca tccggcagcg
    12421 ggttacgggg ccgggccgga ctgccctttg ccaggatgta agcgcattcg tggcggtacg
    12481 cgacatacgc agatttggat gtgtagtttt tggtgaacac gaggtgccct gccacgctga
    12541 atccggcgcg tatccagacc gccataaagc ggtcaatacg gtgccagccg tagaagctga
    12601 ccatcagcgc gtcttttttg agtacgcggt acatctcatt gctggcaggt tgcagccagt
    12661 catcattaac gtcaccggca atggtgcgcc cggaacggtc acggaaaccg acgaggtacg
    12721 gcgggtcagt cagtatgaaa tcaatggcgt tatcaggaat acgggccata acatcgatgc
    12781 agttaccgag gacaaaacgg gacatacaaa actccttctc ttacagaggg caccgggagg
    12841 gcacccctgt ggtgcccgtt acccgctgcc tgtgggctgt tcaccgcgtc cggtaacggg
    12901 tcaggccgac cgtggaatac cgcagccttc gtctttttga cggcaaggct atgccgcgaa
    12961 agcggcttga agggcgttca tcagaaaagg gcgacgggct ggcgtacact gtcaccgacg
    13021 ccagccggga gcggtaaaag cgcccgtccc gtgcccgccg gacgcgggac actgaccaca
    13081 gacagcgttt atgccgcgcc gcgtcagccg gcgcagtaaa aagaggagca cggcgacatc
    13141 ctttaccgga aaagaaacgg gcagttgccc gcccgtctcc gtcatacttc atccgccata
    13201 atgcgccacc ttgtcgacta tccacgcagg tccggtgact tccggcaact cgtcatcatc
    13261 tgtcggatgt gaccattcca gctcccccca cagcacatca acaagctcgc cacgctcata
    13321 tcgctgccag ccacagaaat tgcagccctg ttcggcatac cagtgctcca gtgtgcagct
    13381 gaaacggcgg cttaattctg caataacgtc gctttccggc tgataccacg gcgtgtcgaa
    13441 atcaacctga atgaagttgt ctccctgact actgatattc acctcatacc ccacaggcca
    13501 cttcacgcca tactgttccg tataccagtg atatgcagaa ggaacaccgt tcagaacacc
    13561 accgttaaag ccattcactt caacatccag gcgggtcggc agaaccgtca gcaggtcaaa
    13621 cggcatggtt ttttccggca gtacgttgtc acacagacgg ttccaccaca ccgatacatc
    13681 ctcgttactc cagaagccac accagtcacc tctttttgcg gtgaaaagtg cggtgatggt
    13741 atccctgaca tcgtccggta atccttccca gcgacgctgt ccagtaccgc tctgcagcca
    13801 gagttcatgc agcatcaggc agttttgctc atccagcagt acaccgttct gcaggtatgt
    13861 cagccagcgg gtgaacgcga tattctccgg cgatacaacg ccacgcccgg cagccgtcag
    13921 tccggggcat ggttcaaacc gcacatcttc agtggtctgc agaagtccgg cacttccggc
    13981 cagaaacagc tgaatacctt catttgtggc gcggcgataa agcggtgtga cggcaccgct
    14041 ggccagtcgt ttaatctcag caatctgtgc cggttcacca gaaaaatgca tacgatttga
    14101 gcaccagttt ggcatgggaa ttctcctctc acatgcggcc tggttaagta gtttccgtct
    14161 cagttcgaaa cgcaaaatga gaaatcgata gagactcaaa ctgagtcaca acaagaatgc
    14221 aaagagactc taaaagagcc tcatcgcttg acatgaaccc aaaatgagac acaataagga
    14281 acaacaaaaa actcaaaaag atgcatctag ataaaggatg ctcaatttga gataaataaa
    14341 aactcaaaac gaggcaagaa aaaatgctaa aagtatcatg cgatgatggt tcaactaacg
    14401 taaaactggc ctggcttgaa gatggcgagg taagaaccag tctgtcagga aacagcttta
    14461 aagaaggctg gaacccggga ctgtttaatg ccggcaaagt ttacaattac gtcgtggatg
    14521 gaaaaaaata cacctatgat ttgggcagta ctgctgtcat aggtacaaca catgtcagtt
    14581 atcaatacag caccaccaat ttgcttgcca tacatcatgc actgctgaca tccggccttc
    14641 agccacagga tgtagaactg acagtgacat tacctgtaac cgaattcttt gataatgaca
    14701 atcaaccaaa tgaagaaaga atagaaagaa aaaaggcaaa tgttctccgt gagatatctc
    14761 tgaataaagg ggaaacattt aaaattaaaa aagttaatgt aatgcctgaa tctttgccag
    14821 ctgcatttga atcactaaaa aaagataaag taaacaaact tgaacgcagc ctgattatcg
    14881 atcttggagg aaccactctg gattgtggtt taattctcgg tgcatttgaa ggcatttctg
    14941 aaatccgtgg gtattcagaa atcgggacat cccgcatcac tcataccgtc atgaatgcgc
    15001 tgatgaaagc ctccacacca tgtaattatt ttattgctga tgagttaata aaaaacagac
    15061 atgataatga atatctccag acattaataa atgatgtagc tgaaataaaa aacattactc
    15121 atgtaataga cagggaagtg aaatctctgg cagaaagcat caggcaggaa atttcaacat
    15181 tctcaggaat gaaccgaatc tatctcactg gcggaggagc cgagcttatt taccctcaca
    15241 taaagcaata tttcccgaat ctgaaagtga acaaggtgga tgaacctcag tttgcattgg
    15301 tgaaagcaat ggtgcatgcg taatggaaag cagtgatccg aaaaaaagaa agaaagttgt
    15361 ggcatacctg cacccgacac tttatccgca ggataacctg acacaacaaa caatagactc
    15421 tctcccgata caaatgcggg gagacttcta cagacaatct cttatttgcg gagcagctct
    15481 ttattctgtt gacccccgac tgttaacgct aatcagtggt tttttcagtg agaaaatcac
    15541 tgcagaaaat ctggtgaaac ttatagagca aacaacagga tacacatcta catcaattga
    15601 tataagtgtg ttaaaaaaca tcataggggc atcttcagaa aacaaatcag agagcatcac
    15661 ttcaaaagat gatttcgaag aacaaaccag acgcaatctt tctatgttaa aaaaataata
    15721 aaagaagagc aggttaagtc tattacctgc tcttctttta ttatgaggga ccagcagcgg
    15781 ttttagtgcc ctaaat
//
LOCUS       DAZGOB010000116        15795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGOB010000116 DAZGOB010000000
VERSION     DAZGOB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.116"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(6..1268)
                     /locus_tag="ACHLIF_003845"
     CDS             complement(6..1268)
                     /locus_tag="ACHLIF_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9607769.1"
                     /translation="MKLNARQVETAKPKEKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPMVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRILRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFSEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDAAKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHTERNNVRAAYIHTSEHLDERRL
                     MVQWWADFLEANRNNSVTPYEFAKFMGK"
     gene            complement(1432..1516)
                     /locus_tag="ACHLIF_003846"
     tRNA            complement(1432..1516)
                     /locus_tag="ACHLIF_003846"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1480..1482),aa:Leu,seq:caa)
     gene            1712..2731
                     /gene="ahr"
                     /locus_tag="ACHLIF_003847"
     CDS             1712..2731
                     /gene="ahr"
                     /locus_tag="ACHLIF_003847"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO9607770.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGTDKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2861..4363
                     /gene="yjgR"
                     /locus_tag="ACHLIF_003848"
     CDS             2861..4363
                     /gene="yjgR"
                     /locus_tag="ACHLIF_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO9607771.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDLMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4482..5564)
                     /gene="lptG"
                     /locus_tag="ACHLIF_003849"
     CDS             complement(4482..5564)
                     /gene="lptG"
                     /locus_tag="ACHLIF_003849"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO9607772.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5564..6664)
                     /gene="lptF"
                     /locus_tag="ACHLIF_003850"
     CDS             complement(5564..6664)
                     /gene="lptF"
                     /locus_tag="ACHLIF_003850"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO9607773.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMCTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6727..6777)
                     /gene="ytgA"
                     /locus_tag="ACHLIF_003851"
     CDS             complement(6727..6777)
                     /gene="ytgA"
                     /locus_tag="ACHLIF_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO9607774.1"
                     /translation="MSIIILATISTTQENK"
     gene            6931..8442
                     /gene="pepA"
                     /locus_tag="ACHLIF_003852"
     CDS             6931..8442
                     /gene="pepA"
                     /locus_tag="ACHLIF_003852"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO9607775.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8796..9239
                     /gene="holC"
                     /locus_tag="ACHLIF_003853"
     CDS             8796..9239
                     /gene="holC"
                     /locus_tag="ACHLIF_003853"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO9607776.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTC
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9239..12094
                     /gene="valS"
                     /locus_tag="ACHLIF_003854"
     CDS             9239..12094
                     /gene="valS"
                     /locus_tag="ACHLIF_003854"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO9607777.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12148..13344)
                     /gene="yjgN"
                     /locus_tag="ACHLIF_003855"
                     /pseudo
     CDS             complement(12148..13344)
                     /gene="yjgN"
                     /locus_tag="ACHLIF_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
     gene            13537..14040
                     /gene="yjgM"
                     /locus_tag="ACHLIF_003856"
     CDS             13537..14040
                     /gene="yjgM"
                     /locus_tag="ACHLIF_003856"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9607778.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(14086..14502)
                     /gene="rraB"
                     /locus_tag="ACHLIF_003857"
     CDS             complement(14086..14502)
                     /gene="rraB"
                     /locus_tag="ACHLIF_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO9607779.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14664..15677
                     /gene="argF"
                     /locus_tag="ACHLIF_003858"
     CDS             14664..15677
                     /gene="argF"
                     /locus_tag="ACHLIF_003858"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO9607780.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGAHVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         4040 a         4004 c         4099 g         3652 t
ORIGIN      
        1 ttacattatt tccccatgaa tttagcaaat tcataaggtg taacagagtt atttcgattg
       61 gcctcaagaa aatcagccca ccattgaacc attaatctac gttcgtctaa atgctcagag
      121 gtatgaatat atgctgcacg cacattgtta cgctctgtat gactcaactg ccgttctatc
      181 gcgtcatcgc tccataagcc tgattcacca agcgcgccac gcgccatcgt cctaaatcca
      241 tgcccacaga cctcagtttt cgtgtcatac cccatcgcac gcaaggcgct atttactgtg
      301 ttctcactca taactttggc cgcgtcgtga tcccctggaa aaagtaactc tttatcgcca
      361 ctaatctgct tgagttgctc taacaaaacc atcgcctgtc gactaagagg aacgatatgc
      421 tcctctttca tcttcatgcc acgatacgag taacgcacgc ctttaatttc ttctcgcttt
      481 gcaggtatac gccaaagaga tttatcgaag tcgaactcat cccatcgcgc gaaacgtaac
      541 tcactggaac gtacaaaagt tagtaaggaa agctcgaccg cgatccttgt cattacacga
      601 ccacgatatg cagcaagacg tgcaagaaac tcagagaagc ggctagaggg taaagctgga
      661 tagtgtcgtg ctttggttgt agataacgca ccggccatgt cactggctgg attagagtcg
      721 atgtaatcgt tctgtacggc ataacgcata atggccgtga cgcgctgttg caggcgctga
      781 gcgacatcgt gtttgccact ggcatcaact tttttaatcg gggctaacag gtggctggtt
      841 ttaagctgcc gaatgtcgga cgaaccgata tgaggaaaaa tataaagctc aagatagcgc
      901 agaatgcgtg atcgatggtc ttcactccag cgcttattac tggcatgcca ttcacgagca
      961 attgtttcga aagtgtatgc gcccgaattc tcagcctgag cttctttctg ctcggctttt
     1021 gggtcaatgc cctgcactaa gagcttttta gcctcgtcac gctttgctct tgcctgagca
     1081 agtgtcacca tgggccaaac accaaaagcg aggcgatcct cttttttgtc agaggggcgt
     1141 ctgtatttca tgcgccagta tttagaaccc ttagccgaaa cctcgagata caaaccgccg
     1201 ccatcggcca ttttgtaggt tttctctttt ggctttgctg tctcgacctg tctggcgttg
     1261 agcttcattt gggggcacat ttctaatcga agttaagatg cccccaatta tgcccccaat
     1321 agcatccgga tttcaacgga ctagctcgga cagctcagga cagaaaaacc gctacaggca
     1381 ttgattttaa agggtttgct ggattttctc ggatggtctt ggaagtacta atggtgccga
     1441 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     1501 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     1561 cgccaccatg caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta
     1621 cactcagcga tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     1681 ttcccaaaca acaccagaga aggacccaaa aatgtcgatg ataaaaagct atgccgcaaa
     1741 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     1801 tgttgaagtg caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa
     1861 cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtggt
     1921 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     1981 ctggacggcg cgtagctgtg gtcactgcga cacctgtatt agcggtaatc agatcaactg
     2041 cgagcaaggt gcggtgccga cgattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     2101 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     2161 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     2221 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     2281 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     2341 gatgggtacc gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg
     2401 gcagtttgat ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga
     2461 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     2521 tccggccttt acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc
     2581 ttatgagctg cgtaagctga tgcgttttgc cgcccgcagc aaggttgcgc cgaccaccga
     2641 actgttcccg atgtcgaaaa ttaacgacgc catccagcat gtacgcgatg gtaaagcgcg
     2701 ctaccgcgtg gtcctgaaag ccgacttctg atccctttgc cgggagcctg tcgcccggca
     2761 cttaaatctc tttggaaggc gtctcgcaat ctgctgaatt tagcggtgaa gagtgcacag
     2821 ccgttgccta tactccaggc aggaaactgg aggaaatctc atgagcgaac ccctgttaat
     2881 tgcccgcacg ccggacacag aactgttttt actgccggga atggctaacc gtcacgggct
     2941 gattactggc gcaacgggga cgggtaaaac tgtcacgctg caaaaactgg cggagtctct
     3001 atcggaaatc ggcgtgccgg tgtttatggc cgatgtgaaa ggcgatctga ccggtatcgc
     3061 gcaggcagga acggcgtcgg aaaaactgct cgcaaggctt aaaaatatcg gcgtaaatga
     3121 ctggcaaccg catgctaatc cggtggtggt gtgggatatc tttggcgaga aaggccatcc
     3181 ggtgcgggcg acggtttcgg atctggggcc gctgttgctg gcgcggctgt tgaatctcaa
     3241 cgatgtgcaa tctggcgtgc tgaatatcat cttccgaatt gctgacgatc aggggctgtt
     3301 actgctcgac tttaaagatc tgcgggcgat tacccagtac atcggcgata acgccaaatc
     3361 tttccagaat cagtacggaa atatcagtag cgcatcggtt ggtgccatcc agcgcgggct
     3421 gttgtcgctg gaacagcaag gcgcagcaca cttctttggt gagccgatgc tggatatcaa
     3481 agacttgatg cgcaccgatg ccaacggtaa aggcgttatt aatatcctca gcgccgagaa
     3541 gctttatcag atgccgaaac tgtacgccgc cagcctgctg tggatgattt cagagttgta
     3601 tgaacaattg ccggaagcgg gcgatctgga gaagccaaaa ctggtgtttt tcttcgacga
     3661 agcacatctg ctgtttaacg atgcaccgca ggtactgctg gataagattg agcaggtgat
     3721 aaggcttatt cgctcaaaag gcgtgggcgt ctggttcgtt tcgcaaaacc cgtctgatat
     3781 tccggataat gtgctcggcc agctcggtaa tcgcgttcag cacgctttgc gggcttttac
     3841 gccaaaagat cagaaagcgg taaaagctgc ggcgcaaacc atgcgggcca atccggcgtt
     3901 tgataccgaa aaggcaatcc aggaactggg gaccggcgag gcgttaatct cgtttctcga
     3961 tgcaaaagga agtccttctg tggtggaacg ggcgatggtg attgcgcctt gttcgcggat
     4021 gggaccggtg acggaagatg agcgcaatgg cttgattaac cactccccgg tgtatggcaa
     4081 gtacgaggat gacgtggacc gggaatccgc ctatgagatg ttgcaaaaag gcgtacaggc
     4141 gagtattgaa cagcaaaata atcctccagc gaaagggaaa gagatagcgg tagatgacgg
     4201 cattcttggt ggattgaagg atattttgtt cggcactacc ggaccacgcg gcgggaagaa
     4261 agatggtgtg gtgcagacaa tggccaaaag cgccgctcgc caggtgacga atcagattgt
     4321 acgcgggatg ttggggagtt tgctgggagg gagaagaagg taagtcaggc gcgatctgtc
     4381 cccctcaccc taaccctctc cccaaagggg cgaggggacc gatcgaactc gtatttgact
     4441 ttatcaacaa tgtgaaaggg agctttcgct cccttgcttg gttacgattt tctcattaac
     4501 agccacaggc tgattaagaa gaagctggcg cttggcaaca gtgcgccgat aatcggcggg
     4561 atgccgtaaa ccaacgtcag cgggccgaag atctggtcca gtacgtagaa gacaaaaccg
     4621 aagctgatac cggtgaccac acgcacgccc atcggtacgc tacgcagtgg gccaaagatg
     4681 aacgacagcg ccatcagcat catcaccgcc acggatagcg gctggaagat tttgctccac
     4741 atattgagct gataacgtcc ggcatcctga ccgctcgact tcagatactt cacgtagttg
     4801 tgcaaaccgc tgattgagag tgcatccggg tccagcgcca ccacgcccag tttgtctggc
     4861 gtgaggttgg ttttccaggt gccgctcacc gtctgcgaac cggtgatctg tttcggattg
     4921 gtcagatcag attcatcaac ctgcgacaga cgccagactt tatgttccgg gtcaaacttc
     4981 gcagtagcgg catagcgtac ggattgcaga cgacgatttt cgttaaaggc ataaatgctg
     5041 atgccaccta actcttcgtc acctttaacc cgctcaatgt agacgaagtt gttgccatct
     5101 ttcgcccata agccttgctg ggtagagagc aacgagccgc cgtacatcgc ctgcgcacgg
     5161 tagttacgcg ccatctgctc gccctgcggc gcgacccatt cgccaatcgc catcgtcagc
     5221 aagaccagcg gaatggcggt tttcatcacc gacagcgcca cctgcatacg ggtaaaacca
     5281 gaagcctgca tcaccaccag ttcgctgcgc tgcgccagca tcccaagacc aagcaacgcc
     5341 ccaagcagag ccgccatcgg gaagaagatt tgcacatctt tcggcacgct cagcaaggta
     5401 tacattcctg cgcctaacgc gtcgtaactc ccctgcccgg cttttttcaa ctgatcgaca
     5461 aatttgataa tgcccgacag cgacaccagc atgaacagcg tcatcatgat ggtggtgaaa
     5521 atagttttac cgatatagcg gtcaagtacg ccaaaaggtt gcatcacacc gctcctttac
     5581 gcgaaaaact ggcgcgcagg cggcggaccg gcacggtgtc ccaaaggttg agaacaatcg
     5641 ccaaagccag ataaatcagg ttaacggtcc acatccacag cgtcgggtcc agcttacctt
     5701 taccgccgtt cgatttcagg gaggtctgga tcaggaagaa aagtagatac agcagcatgg
     5761 ctggcagcat cgacagtacg cgtccctgac gtgggttaac cacgctcagc ggtacgacca
     5821 taagtgccat cataaacacg gtgaatacca acgtgatacg ccagttcagt tctgcgcgag
     5881 cacgatcggt gtcagtgttc cacaatgtgc acatgtccat ctggtcggta tcgttcgggt
     5941 cgagcgccac cgcctggtga ccaatgatcg cctgataatc ctggaaatcc gtaatgcgga
     6001 aatcacgtaa cagtgcagtg ccttcgaagc gcgttccctg gttgagagtg acgacctggg
     6061 agccgtcgcg cagctgggtt aaatgtccgg aatcggccac caccacagaa ggacgtgcat
     6121 taccttttgg tcgaatttgt gcgaggaaca catctttgaa atcgctgccg tcaacgcttt
     6181 cgatgaacag caccgagctg ccattagtcg cttgctggaa ttgcccttgc gccagcgccg
     6241 ccatgccagg gttcgctttc gcttctgcta acacttcatc ctgatgacgc gatgaccacg
     6301 gtcccgccca catcacgtta accgccgcga cgattgccgt gaataccgca aggatcattg
     6361 ccgctttcac cagaaccgct ttgctcaggc cgcaggcatg cattaccgta atttcacttt
     6421 cggtatacag tttgcccagc gtcatcagca gcccgaggaa caggcttaat ggcaggataa
     6481 gctgcgccat ttccggcacg cccaacccga gaagggagag caccagattc gccggaatat
     6541 cgccgtcaac cgctgcgccg aggatcctca ctaacttttg acagaagaag atcaaaagca
     6601 agatgaagag tatcgccagc tggcttttga gcgtctcccg caccagatat cttatgatta
     6661 tcactttaaa tacgcccgta aaaactcgtc ttttgcagga ttttagcttg tttcatggct
     6721 taaacgtcat ttattctctt gagtcgtcga aatcgtcgct aagataatta tactcaacgg
     6781 attcacctct cagattttgt tctgacgtgc caatgccgta ataacgttaa gattaacacg
     6841 aagtcatcgc aacagcggac atgagttacg aaagcttgca attctatctg tagccaccgc
     6901 cgttgtcttt aagattcagg agcgtagtgc atggagttta gtgtaaaaag cggtagcccg
     6961 gagaaacagc ggagtgcctg catcgtcgtg ggcgtcttcg aaccacgtcg cctttctccg
     7021 attgcagaac agctcgataa aatcagcgat gggtacatca gcgccctgct acgtcggggc
     7081 gaactggaag gaaaaccggg gcagacattg ttgctgcacc atgttccgaa tgtactttcc
     7141 gagcgaattc tccttattgg ttgcggcaaa gaacgtgagc tggatgagcg tcagtacaag
     7201 caggttattc agaaaaccat taatacgctg aatgatactg gctcaatgga agcggtctgc
     7261 tttctgactg aactgcacgt taaaggccgt aacaactact ggaaagtgcg tcaggctgtc
     7321 gagacggcaa aagaaacgct ctacagtttc gatcagctga aaacgaacaa gagcgaaccg
     7381 cgtcgtccgc tgcgtaaaat ggtgttcaac gtgccgaccc gccgtgaact gaccagcggt
     7441 gagcgcgcga tccagcacgg tctggcgatt gccgccggga ttaaagcagc aaaggatctt
     7501 ggcaatatgc cgccgaatat ctgtaacgcc gcttacctcg cttcacaagc gcgccagctg
     7561 gctgacagct acagcaagaa tgttatcacc cgcgttatcg gcgaacagca gatgaaagag
     7621 ctggggatgc attcctatct ggcggtcggt cagggttcgc agaacgaatc gctgatgtcg
     7681 gtgattgagt acaaaggcaa cgcgtcggaa gatgcacgcc caatcgtgct ggtgggtaaa
     7741 ggtttaacct tcgactccgg cggtatctcg atcaagcctt cagaaggcat ggatgagatg
     7801 aagtacgata tgtgcggtgc ggcagcggtt tacggtgtga tgcgtatggt cgcggagcta
     7861 caactgccga ttaacgttat cggcgtgttg gcaggctgcg aaaacatgcc tggcggacga
     7921 gcctatcgtc cgggcgatgt gttaaccacc atgtccggtc aaaccgttga agtgctgaat
     7981 actgacgctg aaggccgcct ggtactgtgc gacgtgttaa cttacgttga gcgttttgag
     8041 ccggaagcgg tgattgacgt ggcgacgctg accggtgcct gcgtgatcgc gctgggtcat
     8101 cacattaccg gtctgatggc gaaccataat ccgctggccc atgaactgat tgccgcgtct
     8161 gaacaatccg gtgaccgcgc atggcgctta ccgctgggtg acgagtatca ggaacagctg
     8221 gagtccaatt ttgccgatat ggcgaacatt ggcggtcgtc ctggtggggc gattaccgca
     8281 ggttgcttcc tgtcgcgctt tacccgtaag tacaactggg cgcacctgga tatcgccgga
     8341 accgcctggc gttctggtaa agcaaaaggc gcaaccggtc gtccggtagc gttgctggca
     8401 cagttcctgt tgaaccgcgc tgggtttaac ggcgaagagt aattgcgtca ggcaaggctg
     8461 ttattgccgg atgcggcgtg aacgccttat ccgacctaca cagcactgaa ctcgtaggcc
     8521 tgataagacg cgcaagcgtc gcatcaggca atgctgttat tgtcggatgc ggcatgaacg
     8581 ccttatccga cctacacagc accgaactcg taggcctgat aagacacaac agcgtcgcat
     8641 caggcaatgc tgttattgtc ggatgcggcg tgagcgcctt atccgaccta cacacagcac
     8701 tgaactcgta ggcctgataa gacacaacag cgtcgcatca ggcgctgcgg tgtatacctg
     8761 atgcgtattt aaatccacca caagaagccc catttatgaa aaacgcgacg ttctaccttc
     8821 tggacaatga caccaccgtc gatggcttaa gcgccgttga gcaactggtg tgtgaaattg
     8881 ccgcagaacg ttggcgcagc ggtaagcgcg tgctcatcgc ctgtgaagat gaaaagcagg
     8941 cttaccggct ggatgaagcc ctgtgggcgc gtccggcaga aagctttgtt ccgcataatt
     9001 tagcgggaga aggaccgcgt ggcggtgcgc cggtggagat cgcctggccg caaaaacgca
     9061 gcagcagccc gcgcgatata ttgattagtc tgcgaacatg ctttgcagat tttgccaccg
     9121 ctttcacaga agtggtagac ttcgttcctt atgaagattc tctgaaacaa ctggcgcgcg
     9181 aacgctataa agcctaccgc gtggctggtt tcaacctgaa tacggcaacc tggaaataat
     9241 ggaaaagaca tataacccac aagatatcga acagccgctt tacgagcact gggaaaagca
     9301 gggctacttt aagcctaatg gcgatgaaag ccaggaaagt ttctgcatca tgatcccgcc
     9361 gccgaacgtc accggcagtt tgcatatggg tcacgccttc cagcaaacca tcatggatac
     9421 catgatccgc tatcagcgca tgcagggcaa aaacaccctg tggcaggtcg gtactgacca
     9481 cgccgggatc gctacccaga tggtcgttga gcgcaagatt gccgcagaag aaggtaaaac
     9541 ccgtcacgac tacggccgcg aagctttcat cgacaaaatc tgggaatgga aagcggaatc
     9601 cggcggcacg attacccgtc agatgcgccg tctcggcaac tccgtcgact gggagcgtga
     9661 acgcttcacg atggacgaag gcctgtccaa tgcggtgaaa gaagttttcg ttcgtctgta
     9721 taaagaagac ctgatttacc gtggcaaacg cctggtaaac tgggacccga aactgcgcac
     9781 cgctatctct gacctggaag tggaaaaccg cgaatcgaaa ggttcgatgt ggcacatccg
     9841 ctatccgctg gctgacggtg cgaaaaccgc agacggtaaa gattacctgg tggttgcgac
     9901 tacccgtccg gaaaccctgc tgggcgatac cggtgtagcc gttaacccgg aagatccgcg
     9961 ttacaaagac ctgattggca aatatgtcat tctaccgctg gttaaccgtc gtattccgat
    10021 cgttggcgac gaacacgccg acatggaaaa aggcaccggc tgcgtgaaga tcaccccggc
    10081 gcacgacttt aacgactatg aagtcggtaa acgtcacgcc ctgccgatga tcaacatcct
    10141 gacctttgac ggcgatatcc gtgaaagcgc ccaggtgttc gataccaaag gtaacgaatc
    10201 tgacgtttat tccagcgaga tcccggcaga attccagaaa ctggagcgtt ttgccgcacg
    10261 taaagcagtc gttgctgcgg ttgacgcact cggcctgctg gaagaaatta aaccgcacga
    10321 cctgaccgtt ccttacggcg accgtggcgg cgtagttatc gaaccaatgc tgaccgacca
    10381 gtggtacgtg cgtgccgatg tactggcgaa accggcggtt gaagcggttg agaacggcga
    10441 cattcagttc gtaccgaagc agtacgaaaa catgtatttc tcctggatgc gcgatattca
    10501 ggactggtgt atctctcgtc agctgtggtg gggtcaccgt atcccggcat ggtatgacga
    10561 agcgggtaat gtttatgttg gccgcaacga agaagaagtg cgcaaggaaa ataacctcgg
    10621 tgccgacgtt gccctgcgtc aggacgaaga cgttctcgac acctggttct cttctgcgct
    10681 gtggaccttc tctacccttg gctggccgga aaacaccgac gccctgcgtc agttccaccc
    10741 aaccagcgtg atggtatccg gcttcgacat catcttcttc tggattgccc gcatgatcat
    10801 gatgaccatg cacttcatca aagatgaaaa tggcaaaccg caggtgccgt tccacactgt
    10861 ttacatgacc ggtctgattc gtgacgacga aggccagaaa atgtccaaat ccaagggtaa
    10921 cgttatcgac ccgctggata tggttgacgg tatttcgctg ccagaactgc tggaaaaacg
    10981 taccggcaat atgatgcagc cgcagctggc ggacaaaatc cgtaagcgca ccgagaaaca
    11041 gttcccgaac ggcattgagc cgcacggcac cgatgccctg cgcttcaccc tggcggcgct
    11101 ggcgtctacc ggtcgtgaca tcaactggga tatgaagcgc ctggaaggtt accgtaactt
    11161 ctgtaacaag ctgtggaacg ccagccgctt tgtgctgatg aacacagaag gtcaggattg
    11221 cggctttaac ggcggcgaaa tgacactgtc gctggcggac cgctggattc tggcagagtt
    11281 caaccagacc atcaaagcgt accgcgaagc gctggacagc ttccgcttcg atatcgctgc
    11341 aggcattctg tatgagttca cctggaacca gttctgtgac tggtatctgg agctgaccaa
    11401 gccggtaatg aacggtggca ccgaagccga actgcgcggt actcgccata cgctggtgac
    11461 tgtactggaa ggtctgctgc gcctcgcgca tccgatcatt ccgttcatca ccgaaaccat
    11521 ctggcagcgt gtgaaagtac tttgcggtat cactgccgac accatcatgc tgcagccgtt
    11581 cccgcagtac gatgcatctc aggttgatga agccgcactg gccgacaccg aatggctgaa
    11641 acaggcgatc gttgcggtac gtaacatccg tgcagaaatg aacatcgcgc cgggcaaacc
    11701 gctggagctg ctgctgcgtg gttgcagcgc ggatgcagaa cgtcgcgtaa atgaaaaccg
    11761 tggcttcctg caaaccctgg cgcgtctgga aagcatcacc gtgctgcctg ccgatgacaa
    11821 aggtccggtt tccgttacga agatcatcga cggcgcagag ctgctgatcc cgatggctgg
    11881 cctcatcaac aaagaagatg agctggcgcg tctggcgaaa gaagtggcga agatcgaagg
    11941 cgaaatcagc cgtatcgaga acaagctggc gaatgaaggc tttgtcgccc gcgcaccgga
    12001 agcggtcatc gcgaaagagc gtgagaagct ggaaggctat gcggaagcga aagcgaagct
    12061 gattgaacag caggctgtta tcgccgcgct gtaatcaaca ccgtgatgaa aacgaaggcc
    12121 ggagaatgct ccggcctttt ttatctctta cagaaatggt aaagaaggca taattccccg
    12181 tgaaatactg gctaagaagc ctttatctgg ttgttcttct ttattgatta aaggaaggtc
    12241 atccaaatcg cccagcaaat acgtattttt tgcctgccag tcaatcatcc atattttcag
    12301 cagtggataa gccagaccgc ccgtaatccc ggatatcact accaacgcac acatgcgata
    12361 aagcataccg tggtaagtta aagttgagcg aaaacgaata cgcccatcat tcagtgacag
    12421 gttgctcata aaatgatttc gcaacgacac cgttaaataa ctggtgctaa cagcaatccc
    12481 aaaataatag attaactgcg cgattatcat tttacgttgc atttccataa attgctgtaa
    12541 attctcaata tcatcatttg catacacaga actatcatac gcatttaata tttgatcgaa
    12601 gataatataa ccagcaacag cgagaaaagg taataaagct aaaaatgcga ggatggcata
    12661 ttttatacaa tacgcagtat ctaatttcac cttgaaacga tgtataccga aactggtatt
    12721 gctccagaga aaactcatta ccaggctata taaagtacca ttaaaaatac caatggaaac
    12781 aataccaact attgccatca gaaccacaga tataataaca ctacttgaac tattggcatg
    12841 tagcatcttt gtcgagataa agaaaacagt ccccatccca atggccatta aaatcggcaa
    12901 gaaaaaggtt actcaccaga accctttcat agagcaatta aaactaaatc ttacgccgtt
    12961 gagactagtc atcaaggcct gataacgcag tccttttgct gccataaata taagcaaaac
    13021 caacagtgac aaagtcaaaa cacagccaat aagcggcata tctgctgaca ctgtcattaa
    13081 gattgcgaaa tagaaaaaaa caaaaacaag acaactaaca aaaacattcc caccggtaat
    13141 cccataagaa aatcgttgtc cgttaacctc catattagca tagagataac gcttacattt
    13201 cattaatgcc catggtaaat agatacctag cgttataatc gttaacaaca cattcaccac
    13261 acaaataaga aaatatctct cacctgtacc atgaaaaaca aacgaatggg acggaacatc
    13321 catttcatta ataacttgag ccataaaaat tccctgaatt aaaaaatcca ttgatataag
    13381 ttatagatac agacagtaaa atgttctgcc acgttacaat actcttatca gtaataggct
    13441 ggcaaaaact ttattgcttt ataagatgct tgcgcacgta agaagagagt ggtattaaac
    13501 aaatgtataa ttactttatt tttattgggt taaactatga ataacattgc gccgcaatca
    13561 cctgtaatgc gtcgcctgac gctgcaggat aatcctgcta ttgcccgcgt cattcgtcag
    13621 gtatccgccg aatacggtct taccgctgat aaaggctaca ccgtcgccga tccgaatctt
    13681 gacgagctgt atcaagtgta tagtcagccg ggtcatgcat attgggtggt tgagtacgag
    13741 ggtgaagtgg tcggcggcgg tgggattgcg ccattagcag gtagtgaatc ggacatttgc
    13801 gaactgcaaa agatgtattt tcttccggct attcgcggta aagggctggc gaaaaaactg
    13861 gccttaatgg cgatggagca ggcacgagag atgggcttca aacgctgcta tctggaaacg
    13921 accgcttttt taaaggaagc cattgcgctt tatgagcatt tgggctttga gcatatcgac
    13981 tatgcgcttg gctgcacggg ccatgtcgat tgtgaagtgc ggatgctacg cgaactctaa
    14041 aaaaatgccc gctagtgtgt gctatcgggc attttaatca attaattagt ggcgaacccc
    14101 gtcatcgtct tcatcgacaa aatcttcatc gtcgccgtct tcgccgttgg gatcttcaaa
    14161 gtaagtgccc caaccgtcgt actcaacgtc aaatttctcc gccagcgtca tcagttgttc
    14221 aacctgggca tcgatcagat cggcattcaa cgcgcactcg ctgaggatgt cgcagcaaat
    14281 cacgatatca ccatcttcaa cttccagctc ttctggatcg gtcacttcgt aaccgagttt
    14341 aaacgcttca actgctgctt tttccagggt ttccagatcg tctgcggaaa gatggtgttc
    14401 gatggtatac agcgcgtccg gatcgctgcc atcttccagt aattcttcaa taatcaaacg
    14461 tgtttcttca cgctgttctt ccagttgttc cgggtttgcc atggctcatt cctcttaatg
    14521 tcctgccgat actcttattg tcacacacca ctgacattgc ctccaccttt gtgacaaaga
    14581 tttatgcttt agacttgcaa atgaataatc atccatataa attgaatttt aattcattga
    14641 ggtgttagcc acaggaggga tctatgtccg ggttttatca taagcatttc ctgaaattac
    14701 tcgatttcac gccagctgaa ctcaacagcc tgctgcagtt agccgcgaag ctgaaagccg
    14761 ataagaaaag cggcaaagaa gaagccaaac tcactggtaa aaacatcgcg ctcatcttcg
    14821 aaaaagactc gacccgtacc cgatgctctt tcgaagttgc cgcatatgac cagggtgctc
    14881 acgttactta tctcggccca agcggcagtc agattggtca taaagagtcg attaaagaca
    14941 ctgcccgcgt gctgggtcgc atgtatgacg gtattcagta tcgcggctat ggtcaggaga
    15001 ttgtcgaaac actggcggaa tacgctggcg tgccggtatg gaacggcctg accaatgagt
    15061 ttcaccccac gcagttgctg gcggatctcc tcaccatgca ggagcattta cccggtaaag
    15121 cattcaacga aatgacgctg gtctatgcag gtgacgcacg taacaacatg ggcaattcga
    15181 tgctcgaagc tgcggcgctt accggtctgg atttgcgtct ggtcgcgcca caggcatgct
    15241 ggccggaagc tgcgctggtt acggaatgcc gcgctctggc acagcaaaat ggcgggaata
    15301 ttacgctgac ggaagatgta gctaaaggag ttgaaggtgc tgactttatc tataccgatg
    15361 tatgggtgtc gatgggtgaa gcaaaagaga aatgggctga acgcattgca ttgctgcgtg
    15421 attatcaagt gaacagcaag atgatgcagt tgaccggtaa cccggaggtc aaattcctcc
    15481 actgcctgcc cgcgtttcat gacgaccaaa cgacgcttgg caagaaaatg gcggaagagt
    15541 ttggcctaca tggcggaatg gaagtgactg atgaggtctt cgaatctgcc gccagcattg
    15601 tatttgatca ggcggaaaac cgcatgcata ctatcaaagc ggtgatggtc gcgacgctca
    15661 gtaaattaaa taattaatat ctctatcaag atgccgggaa atacaaaaac atcccggcat
    15721 cacattattt tatgcagtga tagttttttt cttccagcca aatttttcta ttgctatacg
    15781 taaattttcc ggagg
//
LOCUS       DAZGOB010000117        15655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGOB010000117 DAZGOB010000000
VERSION     DAZGOB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.117"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..182
                     /locus_tag="ACHLIF_003859"
     CDS             <1..182
                     /locus_tag="ACHLIF_003859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607781.1"
                     /translation="IDIHFWSMGFLAYRCYCATGYHFHLARIARNHVIRGLYLRDITL
                     QVSPFLASILERDQY"
     gene            complement(434..1525)
                     /gene="ychF"
                     /locus_tag="ACHLIF_003860"
     CDS             complement(434..1525)
                     /gene="ychF"
                     /locus_tag="ACHLIF_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO9607782.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1642..2226)
                     /gene="pth"
                     /locus_tag="ACHLIF_003861"
     CDS             complement(1642..2226)
                     /gene="pth"
                     /locus_tag="ACHLIF_003861"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO9607783.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2504..2782
                     /gene="ychH"
                     /locus_tag="ACHLIF_003862"
     CDS             2504..2782
                     /gene="ychH"
                     /locus_tag="ACHLIF_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO9607784.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2837..4516)
                     /gene="dauA"
                     /locus_tag="ACHLIF_003863"
     CDS             complement(2837..4516)
                     /gene="dauA"
                     /locus_tag="ACHLIF_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO9607785.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4641..5588)
                     /gene="prs"
                     /locus_tag="ACHLIF_003864"
     CDS             complement(4641..5588)
                     /gene="prs"
                     /locus_tag="ACHLIF_003864"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO9607786.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5739..6590)
                     /gene="ispE"
                     /locus_tag="ACHLIF_003865"
     CDS             complement(5739..6590)
                     /gene="ispE"
                     /locus_tag="ACHLIF_003865"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO9607787.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHASFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6590..7213)
                     /gene="lolB"
                     /locus_tag="ACHLIF_003866"
     CDS             complement(6590..7213)
                     /gene="lolB"
                     /locus_tag="ACHLIF_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO9607788.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7427..8683
                     /gene="hemA"
                     /locus_tag="ACHLIF_003867"
     CDS             7427..8683
                     /gene="hemA"
                     /locus_tag="ACHLIF_003867"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO9607789.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8725..9807
                     /gene="prfA"
                     /locus_tag="ACHLIF_003868"
     CDS             8725..9807
                     /gene="prfA"
                     /locus_tag="ACHLIF_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO9607790.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9807..10640
                     /gene="prmC"
                     /locus_tag="ACHLIF_003869"
     CDS             9807..10640
                     /gene="prmC"
                     /locus_tag="ACHLIF_003869"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO9607791.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10637..11029
                     /gene="sirB2"
                     /locus_tag="ACHLIF_003870"
     CDS             10637..11029
                     /gene="sirB2"
                     /locus_tag="ACHLIF_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO9607792.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11033..11842
                     /gene="sirB1"
                     /locus_tag="ACHLIF_003871"
     CDS             11033..11842
                     /gene="sirB1"
                     /locus_tag="ACHLIF_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO9607793.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYVQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11878..12732
                     /gene="kdsA"
                     /locus_tag="ACHLIF_003872"
     CDS             11878..12732
                     /gene="kdsA"
                     /locus_tag="ACHLIF_003872"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO9607794.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12881..12988)
                     /locus_tag="ACHLIF_003873"
     CDS             complement(12881..12988)
                     /locus_tag="ACHLIF_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9607795.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13416..13523)
                     /locus_tag="ACHLIF_003874"
     CDS             complement(13416..13523)
                     /locus_tag="ACHLIF_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9607796.1"
                     /translation="MTLAQFAMIFWHDLAASILAGIITAAIVGWWRNRK"
     gene            complement(13952..14059)
                     /locus_tag="ACHLIF_003875"
     CDS             complement(13952..14059)
                     /locus_tag="ACHLIF_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9607797.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(14464..15564)
                     /gene="chaA"
                     /locus_tag="ACHLIF_003876"
     CDS             complement(14464..15564)
                     /gene="chaA"
                     /locus_tag="ACHLIF_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO9607798.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         3928 a         3956 c         4003 g         3768 t
ORIGIN      
        1 acatcgatat tcatttttgg tcaatgggtt ttctggccta tcgttgttat tgtgctactg
       61 gttatcattt tcatcttgcc aggatcgcac gtaatcacgt tattcgggga ctttatctgc
      121 gggatattac ccttcaggta agtccttttc tcgccagcat attggagcgt gatcaatatt
      181 gatcagctgt gaagggggtg atgtgtagtg gaccaaaact tacgttatat cactactaat
      241 gagatatccg tcatctgaca ctgatgtctt taattattct cgtggttcac tgtaggcaat
      301 aagcacaaaa gtgtaggatg ttacaagaat gattaggact cggtgaaatg aaaaatccac
      361 gcaattgcgt ggattatata tacttttgcg ctcttcatga gatttagcga aacctcatga
      421 gacaacaaat taattagacg ttgaaaagga agttcatcac atcgccatct ttaacgatgt
      481 aatctttacc ttctgcacgc attttgcctg cttctttcgc gccttgttca cctttgtaag
      541 tgatgaaatc ttcaaacgag atggtttgtg cacggataaa gcctttttca aaatcagtat
      601 ggattttgcc cgctgcctgc ggcgcggttg ctccaaccgg aatggtccat gcacgcactt
      661 ctttcacccc agcggtgaag taagtttgca ggttcagcag cttataaccg gcacggatca
      721 cacggttcag gcccggctct tccagcccaa gctcctgcat aaactcgtca cgttcttcgt
      781 cgtccagttc ggcaatgtct gcttcaacag cagcacaaac cggaaccaca acagaacctt
      841 ctttcgccgc gatttcacgc acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt
      901 tgacgttggc gatgtacatt gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg
      961 ctttctcttc agcgcttaaa tccagcgcgc gcagcatacc tgcattttcc aactggggca
     1021 ggcatttttc caggaccgcc agctcagctt tcgcgtcttt atcgccacct ttggctttct
     1081 tctgtacgcg atgaatcgca cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt
     1141 tgataacttc aatatcgtca gccgggttaa ctttgccgga aacgtgaatg atgttgtcat
     1201 tttcaaagca gcgaacaacg tgaccgatcg cttcggtttc acggatgttg gtcaggaact
     1261 ggttacccag accttcgcct ttcgatgcgc cttttaccag accggcgata tcgacaaatt
     1321 ccatggtcgt gggaagcgta cgttgcggtt ttacgatttc agccagttga tctaggcgag
     1381 ggtcaggcat tggtacgacg cctgtgttcg gctcaatggt gcagaatgga aagttggccg
     1441 cttcaatacc ggctttggtc agcgcgttga acagggtaga tttcccgacg ttgggcaaac
     1501 cgacgatacc gcatttgaat cccatgattt aaatcacctt aatatcttaa taatcaacct
     1561 gttattgcta acagattgca gaaatggaaa taactttgcc tattatacac ggcactcggc
     1621 aaaaatgccg cagacaacga cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt
     1681 ggtcaagcca tctgtaaacc acatttcagt acaacgcgcc gcttcgtcga tggcttcatc
     1741 aattaacttc tgttcactaa caggcggttt gcctaacaca aaaccgacaa ctttattttt
     1801 atcgcccgga tgaccgattc cgatgcgtaa acggtgaaag ttagggttat tacccaattt
     1861 actgatgatg tctttcagtc cattgtgacc accatggcca ccgcccaatt taaatttggc
     1921 gacgccagga ggcagatcca gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg
     1981 gaaaaaactg gccatcgccg caacggcttt gccgctgaga ttcataaatg tagtcgggac
     2041 taacaggcgg acatcttcgc ctccaagagt gactcgcgaa gtataaccaa agaatttagc
     2101 ctcttcgcgc agcggagcgc gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc
     2161 attatgtcgc gttgcggcgt attcagcacc ggggttcgcc aggccgacaa tcaatttaat
     2221 cgtcacgttt ttttgtcctg agtgtgtaca taactggcgc gtagtttact ggttgcggcc
     2281 ccgcttgaca aaaaactgcg tatcaaatgc agataacgta ataattgcat gagtggacta
     2341 ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt tgttgaaata agggttgtaa
     2401 ttgtgatcac gcccgcacat aacccacagg gtgttgtcta tactttacac ataaggaaga
     2461 ggggtattcc ctgttacaac ccagaaagtt ccggaggtga catatgaaac gcaaaaacgc
     2521 ttcgttactc ggtaacgtgc tcatggggtt gggtctggtg gtaatggtgg tcggcgtggg
     2581 gtattcaatc ctcaaccagt taccacagtt taatatgccc cagtatttcg cacatggtgc
     2641 agtgctaagt attttcgtcg gtgccattct ctggctggcg ggtgcccgtg ttggcgggca
     2701 tgaacaggtg tgcgaccgtt actggtgggt tcgccactat gacaaacgtt gccgccgtag
     2761 cgataatcgc cgtcatagct aacaaataat gcagtttgct gaccagtcag tttgcgctga
     2821 ctggtcaatc tcgtacttat aaatccgcca tcgccgcgcg acgattcggg aagaacgcca
     2881 ggcgtcccgg gatcggttga atgccagcgc gagccatagt gcgcagtggc tggaattcca
     2941 cgttgcacac gcgcagttca catccctcgg gcagacgctt cacaaaacgc tggaacgcat
     3001 caagaccacc agcatcaagt accggaaccg catcccactt cagaatcaca atccgtttgc
     3061 cttcaagacg tgactccagg tccgtgaaca agccttcagc agcagcaaaa aacagcgggc
     3121 caataacgcg cagaaccagg acatcgtctg gaacatctac gactaccggt gccaggcgag
     3181 tcatacgtgc gatacgacgc ataaacagca gcgatgccag cacgatcccc acgctgatgg
     3241 caataaccat atcaaacagc acggtcagcg acatgcacag cagcatgaca atgatgtcat
     3301 ctttcggcgc atgacgcagc aagtcgacca ctttgtgcgc ttcactcatg ttccacgcca
     3361 ccatcaacag cagggcagcc atggcggaaa gcggcagcca ggagagcagc ggtgccagta
     3421 ccagcagggc aagaataacc agaatagagt ggatcaccgc cgagataggg gaagttgccc
     3481 cggcacggac gttagcggca gaacgcgcga tggcagctgt agcggtaatg ccaccaaaga
     3541 acggagcgat gatattcccc agtccctgtc caaccagttc gctattcgcc ttgtgtttcg
     3601 tcccggtcat accatccagc accacggcgc agagcagaga ttcgattgcg ccgagcattg
     3661 ccattgagaa tgccgcaggc agcagtgtgc gaatagaatc ccaggttagc gtgaattctg
     3721 aattaggcag atcccacggc agcaccagtt gcggcagcag ttgcggaata ccgttaccct
     3781 gagaaccatc ggccaggacg tagtggaatt gcgaaccgat ggtagcaaca tgtccgccga
     3841 gcaggttaac aatccccatc accgcgcaac cagccagcaa ggccggaagg tgaccgggta
     3901 aacgaatgcc cagacgcggc cagaaaacaa gaatacctag cgtcacaatg ccaatggcag
     3961 catcacccac attaatggtc ggcagcgcca taaataatgc gccgactttt tgtagataat
     4021 gttccgggac atgggccatt tgcagaccga gaaaatcttt aatctgcatg gtaccgatgg
     4081 tgatcccgat acccgaggtg aaacctaagg tgacggaaac cggaatatac tcaatcaggc
     4141 gaccaaagcg tgccagaccc atcagaatca aaaaaatccc cgacagcaag gtcgcaacca
     4201 gcagtcctgc cagtccaaac tgttgcgaca cgggatagag aattaccaca aatgccgcag
     4261 tcggaccgga aacgctaaag cgtgacccac ccgtcagagc aatgacaatc cccgcaacag
     4321 ctgcggtata taaaccgtac tggggtgcca caccactacc aatagccaac gccatcgcca
     4381 gcgggatagc aataatcccg acggttatcc cggcaatcag gtcacgggta aaccgtgcgg
     4441 cagtatattt ttctttccag caagcgtcga tcagagcgcg gaaaggcatc acatgtgagg
     4501 aaaatatttt gttcacaata atgtttcatc cgtgagcgca tcatctgtca actaaatggc
     4561 aggtgaagga ggcataggtc atacaaatgg atattacaga caaaaaaacc cgccgcagcg
     4621 ggtctttgag ccgggttcga ttagtgttcg aacatggcag agatcgattc ttcgttgctg
     4681 atacgacgaa tcgcttcggc cagcatacct gacagggtca gagtacgcac gttcggcagt
     4741 gatttgattt catcgctcag cggaatggta tcgcagacaa cgacttcatc aattacagag
     4801 ttacgcaggt tattcgccgc gttgccagag aagatcgggt gagtcgcgta cgcaaataca
     4861 cgtttagcac cacgttcttt cagagcttca gcagctttac acagcgtacc gccagtgtcg
     4921 atcatatcat cgaccagtac gcagtcacga cctgcaacgt caccgatgat atgcatcacc
     4981 tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg ccatatcggt atcgttcagc
     5041 agcttagcga tagcgcgggc acgcacaacg ccgccgatgt ccggagaaac cacaattggg
     5101 ttatccagat tcagctgcag catgtcttcc agcaggatcg ggctaccaaa tacgttatca
     5161 accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca gatccactgt cagcacacgg
     5221 tcaacaccga cgctggagag gaagtctgca accactttcg cagtgattgg tacacgagcg
     5281 gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg ggataacagc ggtgatacga
     5341 cctgcggaag cacgacgcag ggcatcaacc ataacgacta attccatcag gttgtcgtta
     5401 gtaggggcac aagtggactg gatgatgaaa atatcaccac cgcgtacatt ttcgttgatt
     5461 tgtacactga cttcgccgtc gctaaagcga cctacagcgg cgtcgccgag tgaagtgtac
     5521 aggcggttgg caatacgttg tgctagttcc ggggtggcgt taccagcaaa aagcttcata
     5581 tcaggcacga gaagaacctc aggcatgcgt ccattggtgg aaagaatctg ccgaaaactg
     5641 tgcgggccag gcatgatcct ttccaggcgg tgtattaaag agcgcgatgc aacgtctgga
     5701 acaaggtgac gttgtcaccg aaactcagct tgcccggctt aaagcatggc tctgtgcaat
     5761 ggggaaagat taacgccttt cgccacaaag ccattgagcc attccggggc ttgctctagc
     5821 acctggcggg cttcagactc tgtatcaaat tcagcaaaga cacaggcccc tgtcccagtc
     5881 aggcgcgacg gggcgtattc taacagccag gaaagcaccg catcaacctc gcgaaaacgt
     5941 tttcttgcga taacctcgca atcattgctg aattcacatt ttagcaacgt ttctattgac
     6001 ctttttggcg tattgcgcgg gagttcagga tctttaaaaa tcaccggagt cggaatactt
     6061 acaccagggt gcgccaccag ataccacttc tctggcggat ccaccggcgt tagtatttca
     6121 ccaacgcctt cggcaaacga ggcatgcccc cgaacaaaga caggaacatc tgcgcccagc
     6181 gtcagcccca tttccgccag ctcatccatg cttagcccgc attgccagag atgatttaat
     6241 gccaccagga ccgtcgcggc attggatgaa ccaccgccga gaccgccgcc catcggcaaa
     6301 cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa gacgcccgct gtctgccgca
     6361 gttttcatca acaatcgcgc tgcgcgaacg atcaggttat cttcatgttc cacgccttca
     6421 acgggcgtta acagacgaat atccccatcg tcacgaagct caatgctgat ggtgtcgccg
     6481 taatcaagaa actgaaacag cgtttgcagc gtgtggtaac catccgcacg ctgaccggta
     6541 atgtataaaa acagattaag ttttgccgga gagggccact gtgtccgcat tatttcacta
     6601 tccagttatc catttttaac ttgatgcgtt gaccaccgtc ggtgagttcc atattggctg
     6661 gcatcgcagg ttgcgttttg gtgtcataac caccataaac aaccttccag tttttgccat
     6721 tctggctgta ggtaatttcg ctcaggcggt actggtcgtc cagtttgtag tcggttgcat
     6781 cacccggtaa acctaaaatc cactggcgca agctgttgag cggaattggc attccggtca
     6841 atttgccaat catctcttcg gcgtcatcgg cggtataacg ctgaccttta ttgtcgacta
     6901 actgcacgtt acccggttga gcattcagct ccagttccgt gctgcccaat gggttagtga
     6961 gcagcagacg gtagcgatcc tggccggttt gctgccagaa aaagcgggcg tacacttttt
     7021 gttggtcaga aatataagcg aacgcgccgc gagtctgata ctgattaaga ttgcgcacgt
     7081 cttgctgatg ctgacgccat tgtggcgaat ccgggctttt gccaggacct ttgggcgtgg
     7141 taacggaaca ggcagtgagc acaagagcag ccagcggtag caggcggata agacgaaaat
     7201 cgggcagggg catagtgatg acaagtcctt gagatacgtt gcagttataa cccttaatgc
     7261 tagcgttacc gtccgctatc gtctatgttc aagttgtctt aattgccaga atctaacggc
     7321 tttcggcaat tactccaaaa gggggcgctc tcttttattg atcttacgca tcctgtatga
     7381 tgcaagcaga ctaaccctat caacgttggt attatttccc gcagacatga cccttttagc
     7441 actcggtatc aaccataaaa cggcacctgt atcgctgcga gaacgtgtat cgttttcgcc
     7501 ggataagctc gatcaggcgc ttgacagcct gcttgcgcag ccgatggtgc agggcggcgt
     7561 ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc gttgaagagc aggataacct
     7621 gcaagaggcg ttaatccgct ggctttgcga ttatcacaat cttaatgaag aagatctgcg
     7681 taaaagcctc tactggcatc aggataacga cgcggttagc catttaatgc gtgttgccag
     7741 cggcctagat tcactggttc ttggggagcc gcagatcctc ggtcaggtta aaaaagcgtt
     7801 tgccgattcg caaaaaggtc atatgaaggc cagcgaactg gaacgcatgt tccagaaatc
     7861 tttctctgta gcgaaacgcg ttcgcactga aacagatatc ggtgccagtg ctgtgtctgt
     7921 cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa tcgctctcta cggtcacagt
     7981 gttgctggta ggcgcgggcg aaaccatcga gctggtagcg cgtcatctgc gcgaacataa
     8041 agtacagaag atgattatcg ccaaccgcac tcgcgaacgt gcccaaattc tggcagatga
     8101 agttggcgcg gaagtgattg ccctgagtga gatcgacgaa cgtctgcgcg aagccgatat
     8161 catcatcagt tccaccgcca gcccgttacc gattatcggg aaaggcatgg tggagcgcgc
     8221 attaaaaagc cgtcgcaacc aaccaatgct gttggtggat attgccgttc cgcgcgatgt
     8281 tgagccggaa gttggcaaac tggcgaatgc ttatctttat agtgtggacg atctgcaaag
     8341 catcatttcg cacaacctgg cgcagcgtaa agccgcagcg gttgaggcgg aaactattgt
     8401 cgctcaggaa accagcgaat ttatggcgtg gctgcgagca caaagcgcca gcgaaaccat
     8461 tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag ttaaccgcca aagcgttagc
     8521 ggcccttgag cagggcggcg acgcgcaagc cattatgcag gatctggcat ggaaactgac
     8581 taaccgcttg atccatgcgc caacgaaatc acttcaacag gccgcccgtg acggggataa
     8641 cgaacgcctg aatattctgc gcgacagcct cgggctggag tagcagtaca tcattttctt
     8701 tttttacagg gtgcatttac gcctatgaag ccttctatcg ttgccaaact ggaagccctg
     8761 catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg cgcaaactat cgccgaccag
     8821 gaacgttttc gcgcattatc acgcgaatat gcgcagttaa gtgatgtttc gcgctgtttt
     8881 accgactggc aacaggttca ggaagatatc gaaaccgcac agatgatgct cgatgatcct
     8941 gaaatgcgtg agatggcgca ggatgaactg cgcgaagcta aagaaaaaag cgagcaactg
     9001 gaacagcaat tacaggttct gttactgcca aaagatcctg atgacgaacg taacgccttc
     9061 ctcgaagtcc gagccggaac cggcggcgac gaagcggcgc tgttcgcagg cgatctgttc
     9121 cgtatgtaca gccgttacgc cgaagcccgc cgctggcggg tagaaatcat gagcgccagc
     9181 gagggtgaac atggtggtta taaagagatc atcgccaaaa ttagcggtga tggtgtgtat
     9241 ggtcgtctga aatttgaatc tggcggtcat cgcgtgcagc gtgttcctgc tacggaatcg
     9301 cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa tgccagaact gcctgacgca
     9361 gaactgccgg acatcaaccc agcagattta cgcattgata ctttccgctc gtcaggggcg
     9421 ggtggtcagc acgttaacac caccgattcg gcaattcgta ttactcactt gccgaccggg
     9481 attgttgttg aatgtcagga tgaacgttca caacataaaa acaaagcgaa ggcgctttca
     9541 gtgctcggtg ctcgcatcca cgctgctgaa atggcaaaac gccaacaggc cgaagcgtct
     9601 acccgtcgta acctgctggg gagtggcgat cgcagcgacc gtaaccgtac ttacaatttc
     9661 ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc tctaccgcct ggatgaagtg
     9721 atggaaggta agctggatat gctgattgaa ccgattatcc aggaacatca ggccgaccaa
     9781 ctggcggcgt tgtccgagca ggaataatgg aatatcaaca ctggttacgt gaagcaataa
     9841 gccaacttca ggcgagcgaa agcccgcggc gtgatgctga aatcctgctg gagcatgtta
     9901 ccggcagagg gcgtactttt attctcgcct ttggtgaaac gcagctgact gacgaacaat
     9961 gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg tgaacccatt gctcatttaa
    10021 ccggggtgcg agaattctgg tcgttgccgt tatttgtttc gccagcgacc ttaattccgc
    10081 gcccggatac ggagtgtctg gtggagcagg cactggcgcg gttgcctgaa caaccttgcc
    10141 gtattcttga tctcgggacg ggtaccgggg cgattgcgct tgcgctggct agcgagcgcc
    10201 cggactgcga aattatcgct gtagatcgta tgcctgatgc tgtctccctg gcacaacgta
    10261 atgcccagca tctggcgatc aaaaatatcc acattctgca aagcgactgg tttagcgcgc
    10321 tagccgggca gcagtttgcg atgattgtca gcaatccgcc gtatattgac gagcaggacc
    10381 cacatcttca acaaggcgat gtccgctttg agccgctcac tgcgctggtt gcggcagaca
    10441 gtggaatggc agacatcgtg catatcatcg aacagtcgcg taacgcgctg gtatccggcg
    10501 gctttctgct tctggaacat ggctggcagc agggcgaagc ggtgcgacaa gcatttatcc
    10561 tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg tgataacgag cgcgtaacgc
    10621 tcggccgcta ttatcaatga caagtttttc tacactgctt agtgttcatc ttattagtat
    10681 cgcgctttct gttgggctat taaccttacg tttctggcta cgttatcaga agcatccaca
    10741 ggcatttgct cgctggacgc gcattgtgcc gccggttgtc gatacggtgt tattgttaag
    10801 cggcattgcg ttgatggcta aagcgcacat cctgccattt tccgggcagg cacagtggct
    10861 gactgaaaag ctgtttggag ttatcattta tatcgttttg ggttttattg cactcgatta
    10921 tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc ccgctggcgt tggtggtgct
    10981 gtacatcatc attaaactcg ccaccacaaa agtaccgtta ctggggtaag tcatgagatc
    11041 gttagctgat ttcgaattta ataaagcgcc attgtgcgaa ggcatgatcc tggcttgcga
    11101 agcaatccgc cgcgattttc cctcgcaaga tgtttacgac gaactggagc gtctcgttag
    11161 tctggcgaag gaagaaatca gccagcttct gcctttagaa gagcagctgg aaaaactgat
    11221 cgcgctgttt tacggcgact ggggatttaa agcctcacgc ggcgtttatc gtctttccga
    11281 tgcattatgg ctggaccagg tgttaaagaa tcgacagggc agtgcggtat cattaggtgc
    11341 ggttttatta tgggtcgcga atcgtctcga tttgccgctg ctgccggtga ttttccctac
    11401 gcagctgata ttgcgcattg aatgtccgga tggcgaaatt tggctgatta atccttttaa
    11461 cggtgaatcg ttaagcgaac atatgctgga cgtatggtta aagggaaata tcagcccgtc
    11521 ggcggaactg ttttatgaag accttgatga agctgataac attgaggtaa tccgcaaatt
    11581 gctggataca ctcaaagcct cgttgatgga agaaaatcag atggagctgg cgttacgcac
    11641 cagcgaagct ttattacaat tcaaccctga agatccctat gaaattcgcg atcgcgggtt
    11701 gatttatgtg caactggatt gcgaacacgt tgcgttgaac gatttaagtt atttcgttga
    11761 acagtgtccg gaagacccga tcagcgaaat gatccgtgcg caaataaata acatcgcgca
    11821 taaacatatt gtgctgcatt aattaatcga cattttactc aagattaagg cgatcctatg
    11881 aaacaaaaag tggttagcat tggcgacatc aacgtagcaa atgacctgcc gttcgtactg
    11941 tttggcggta tgaacgtgct ggaatctcgc gatctggcga tgcgcatttg cgagcactac
    12001 gtaactgtga cccagaaact gggtatccct tacgtgttca aagcctcttt tgacaaagcc
    12061 aaccgctcct ccatccactc ttatcgtgga ccgggcctgg aagaagggat gaaaatcttc
    12121 caggagttga agcagacttt tggcgtgaaa attatcaccg acgttcacga accaagtcag
    12181 gcacagcccg ttgctgatgt cgtggatgtg attcagttgc cggcgtttct tgctcgccag
    12241 actgacctgg ttgaagccat ggcgaaaacc ggtgcggtaa ttaacgtcaa gaaaccacag
    12301 tttgtcagcc cgggacagat gggtaatatc gttgataaat tcaaagaagg cggcaacgaa
    12361 aaagtgattc tttgcgatcg tggtgctaac ttcggctatg acaacctggt tgtcgatatg
    12421 ctgggcttca gcattatgaa gaaagtgtct ggtaactcgc cggtgatttt cgacgtgacc
    12481 cacgcactgc aatgccgcga tccgtttggc gcagcttccg gtggtcgtcg tgctcaggtg
    12541 gctgagctgg cacgagccgg tatggcggta ggtctggcgg ggctgtttat tgaagcgcat
    12601 ccggatccgg aacatgcgaa atgtgatggt ccatccgcgc tgccgctggc taaactggaa
    12661 ccgttcctca agcagatgaa agcgattgat gatctggtga aaggtttcga agaactggat
    12721 accagcaagt aatctttttt gcttgaaaaa taaagtctta gcgttctgcg ttaagacttt
    12781 tttcatgggt gccagataca aaaaaggccg caggctgtta cccctgcggc cggtttcggg
    12841 cgcatattgc catcacggca gcctgacgcc cgttttcacc ttacttccgg ttacgccacc
    12901 agccgacaat cgctgcggta ataattcccg ccaggatcgg tgctgccagg tcgtgccaga
    12961 aaatcatggc aaactgcgcg agcgtcatat agccgccttg ttgtaatgac aacattttgc
    13021 ggctattctt gaattgttct ggttcaagat tagcccccgt tctgttgtca ggttaaacct
    13081 ctcaacgtgc gggggttttc tctttccagc aaccaatgcc accagggata aagcccccgc
    13141 aacattgcgc ctcaccggat aacgccggct tgatgtggat actacgtctc aattcatctt
    13201 cacttcatcc ctgaaatgtt tgcaatgaag agtgcattcc ggtttttcaa cagctgttac
    13261 agtcgtttca tgagggctct ggatgatgct tccagctcgg gttgccaatg tttactggcg
    13321 gaagagataa agacaaaaaa ggccgcaggc tgttacccct gcggccggtt tcgggcgcat
    13381 attgccatca cggcagcctg acgcccgttt tcgccttact tccggttacg ccaccagccg
    13441 acaatcgctg cggtaataat tcccgccagg atcgatgctg ccaggtcgtg ccagaaaatc
    13501 atggcaaact gcgcgagcgt catatagccg ccttgttgta atgacaacat tttgcggcta
    13561 ttcttgaatt gttgatggtt caagattagc ccccgttctg ttgtcaggtt ttacctctca
    13621 acgtgcgggg gttttctctt tccagcaacc aatgccacca gggataaagc ccccgcaaca
    13681 ttgcgcctca ccggataacg ccggcttggt gtggatacta cgactcaatt catcttcact
    13741 tcatccctga aatgtttgca atgaagagtg cattccggtt tttcaacagc tgttacagtc
    13801 gtttcatgag tgctctggat gatgcttcca gctcgggttg ccaatattta cttgtggaag
    13861 agataaagac aaaaatggcc gcaggctgtt acccctgcgg ccggtttcgg gcgcatattg
    13921 ccatcacggc agcctgacgc ccgttttcac cttacttccg gttacgccac cagctgacaa
    13981 tcgctgcggt aataattccc gccaggatcg gtgctgccag gtcgtgccag aaaatcatgg
    14041 caaactgcgc gagcgtcata tagccgcctt gttgtaatga caacattttg cggctattct
    14101 tgaagtgtct ggtttcaaga ttagcccccg ttttgttgtc aggttttacc tctcaacgtg
    14161 cgggggtttt ctctttccag caaccaatgc caccagggat aaagcccccg caacattgcg
    14221 cctcaccgga taacgccggc ttggtgtgga tactacgtcg caattcatct tcacttcatc
    14281 cctgaaatgt ttgcaataaa gagtacattc cggcttttca acagctgttg cagtggtttc
    14341 gtgcaggttg tggtacaggc tcgcaattct gattaacgag ctgtccaggt agtatgaaga
    14401 aatacaataa gcaggaattt atcttatctc ccccgatttc cgtcatcgga ggagatataa
    14461 ctgtcaggca aatatcgtca tcaaataggc ggcaaacagt gccagatgcg ctgcgccatt
    14521 gagcacgtta gtacgtccgg tggagaagga gatatggcac agcactaaag aggccaccat
    14581 caccaccatt tctggcgcac caagtgcaaa ctgtaattcg ttacccgtca taaaggcaat
    14641 tagcgtgacg acaggtacgg taagcgaaat ggttgctaac actgaaccaa agaacagatt
    14701 catcgcgcgc tggacctggt tgttcaacac tgcttttaac gcacctaaac cttccggcga
    14761 cagaatcaac agagccacca ggaagccagt aaaggcgaca ggggcattca tgctgtcgag
    14821 caatgtctcc agcgggctgg cattcatttt ggtgactgca ataacggcaa tcagatggat
    14881 aatcaaccag atagcatgcc acacgctgct atgggcagac ggtttaccgt gatgcgggtc
    14941 atcgtcatca ctgtcatctt cgtgctcgta gacaaacaaa ctttgatgcg ttttggtctg
    15001 gatcagcaaa aatacgccat acattgccgc agaaattaat gctaccagta acgcctgacc
    15061 ggttgaaaaa ttcgccgcag gcagagccat tggaaatacc agtacgatta tcgccagggg
    15121 gaacagcgca attaaatact gcttgatacc aaacagattc atatattggg tggcaaactt
    15181 acgaccgccc aacaataatg aaaagccaac cagcccaccg gtaacaatca taatgattga
    15241 atagagcgta tcacgcatta gcgttggcgc ggcgtcgccg gttgccatta aagctgaaat
    15301 caaactgact tcaagaataa ccactgaaag gctaagaata agcgaaccgt aaggttctcc
    15361 caggcgatgg gctaatacgt ccgcatgacg gacaacacta aaggcgctac ttaaaatacc
    15421 aataagcgca agaagattga tggcaatgac cactggtagt gtctggctgc ttccccacag
    15481 gaacagcact accagcgcca gaaccgggaa aataagcgaa gtctccttgt ggcgggtttt
    15541 taccgcctct tgagcatttg acattatggt tatccctttg cagatgaatt tatcgaaaat
    15601 gtaaaaaata ggtaggaaaa ataacagaaa gtgtctggat atcggtaaca tttta
//
LOCUS       DAZGOB010000118        15352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGOB010000118 DAZGOB010000000
VERSION     DAZGOB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.118"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            24..1313
                     /gene="hpaG"
                     /locus_tag="ACHLIF_003877"
     CDS             24..1313
                     /gene="hpaG"
                     /locus_tag="ACHLIF_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO9607799.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGCLVNRIVSEETAK"
     gene            1310..2776
                     /gene="hpaE"
                     /locus_tag="ACHLIF_003878"
     CDS             1310..2776
                     /gene="hpaE"
                     /locus_tag="ACHLIF_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9607800.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2778..3629
                     /gene="hpaD"
                     /locus_tag="ACHLIF_003879"
     CDS             2778..3629
                     /gene="hpaD"
                     /locus_tag="ACHLIF_003879"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO9607801.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3639..4019
                     /locus_tag="ACHLIF_003880"
     CDS             3639..4019
                     /locus_tag="ACHLIF_003880"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO9607802.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQHDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4130..4933
                     /gene="hpaH"
                     /locus_tag="ACHLIF_003881"
     CDS             4130..4933
                     /gene="hpaH"
                     /locus_tag="ACHLIF_003881"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO9607803.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4944..5732
                     /gene="hpaI"
                     /locus_tag="ACHLIF_003882"
     CDS             4944..5732
                     /gene="hpaI"
                     /locus_tag="ACHLIF_003882"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO9607804.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPDVY"
     gene            5907..7283
                     /gene="hpaX"
                     /locus_tag="ACHLIF_003883"
     CDS             5907..7283
                     /gene="hpaX"
                     /locus_tag="ACHLIF_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO9607805.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7293..8183
                     /gene="hpaA"
                     /locus_tag="ACHLIF_003884"
     CDS             7293..8183
                     /gene="hpaA"
                     /locus_tag="ACHLIF_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO9607806.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8434..9996
                     /gene="hpaB"
                     /locus_tag="ACHLIF_003885"
     CDS             8434..9996
                     /gene="hpaB"
                     /locus_tag="ACHLIF_003885"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO9607807.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10014..10526
                     /locus_tag="ACHLIF_003886"
     CDS             10014..10526
                     /locus_tag="ACHLIF_003886"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO9607808.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSIT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10919..13069
                     /gene="btsT"
                     /locus_tag="ACHLIF_003887"
     CDS             10919..13069
                     /gene="btsT"
                     /locus_tag="ACHLIF_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO9607809.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13200..13403
                     /locus_tag="ACHLIF_003888"
     CDS             13200..13403
                     /locus_tag="ACHLIF_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO9607810.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13414..14370
                     /gene="yjiA"
                     /locus_tag="ACHLIF_003889"
     CDS             13414..14370
                     /gene="yjiA"
                     /locus_tag="ACHLIF_003889"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO9607811.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(14457..14648)
                     /locus_tag="ACHLIF_003890"
     CDS             complement(14457..14648)
                     /locus_tag="ACHLIF_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO9607812.1"
                     /translation="MSMVKHKRGTLYTLDVQQEAELMALVNKSDEDIDYSDIPASGSE
                     AWSDAKRGNFLPDPGIHAQ"
     gene            complement(14688..>15352)
                     /locus_tag="ACHLIF_003891"
     CDS             complement(14688..>15352)
                     /locus_tag="ACHLIF_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607813.1"
                     /translation="IFSVSASLYVFSAFSVFVFLGSIFVILELEFENNNKLILIRSMI
                     IACIPIIYLFASSCASSLFLSLSNLNITLTPWVEYFWKGMAFMLLFFMLMQLVIYFAF
                     LTLWAKLSVYRLFILAGAFIVSTILVAFASKNVEGISYYVLKSTIDFEWRNQVKCGEL
                     NISRPDERYFGFNTDKYTVFYSNREGKWGVNELKCKKGSDRRDAYSIENVSEYNVPGW
                     LK"
BASE COUNT         3640 a         4177 c         4136 g         3399 t
ORIGIN      
        1 atgatcgaat ccgaggagtg gtaatgaaag gcactatctt cgccgtagcg ttgaaccatc
       61 gcagccaact tgatgcatgg caggaagcgt tccagcaatc cccctacaaa gccccgccta
      121 aaactgcggt ctggtttatt aaaccgcgca atacggtgat tggttgcggt gaaccgattc
      181 cctttccaca gggtgaaaag gtactgagcg gtgcgactgt tgcgctgatt gtgggaaaaa
      241 cggcgacgaa agtacgtgaa gaagatgcgg cagagtacat cgccggatat gcgctggcta
      301 acgacgtcag cctgccggaa gagagctttt accgcccggc aatcaaagca aaatgtcgtg
      361 atggattctg ccccattggc gaaaccgtgg ctctcagcaa tgtcgataat ctgaccatct
      421 ataccgagat caacgggcgt cctgccgatc actggaatac cgccgattta caacgtaacg
      481 ccgcgcagtt gctgagcgcc ctgagcgaat ttgccacact gaatccaggc gatgccattc
      541 tgctcggcac gccacaggcg cgcgtggaaa tacagccagg cgatcgcgtt cgtgttctcg
      601 cagaaggttt cccgccgctg gaaaatccgg tagtggacga acgtgaagtg accacgcgca
      661 agagcttccc aacgcagcca cacccgcacg gcacgctgtt tgccctcggc ctgaactacg
      721 ccgaccacgc cagcgaactg gaatttaagc caccggaaga accgctggtg ttcctgaaag
      781 cgccaaatac cctcactggc gataaccaga cctccgtgcg tccaaacaat attgaataca
      841 tgcactatga agcggagctg gtggtagtta ttggcaaaca ggcgcgtaac gtcagcgaag
      901 ccgatgccat ggattatgtc gcgggctaca ccgtgtgtaa cgactacgcc attcgcgact
      961 atctggaaaa ctactaccgc cctaacctgc gggtaaaaag ccgcgacgga ctgacgccga
     1021 tgctttcaac catcgtgccg aaagaggcga tcccggaccc gcataatctg acccttcgca
     1081 ccttcgtcaa cggcgagtta cgccagcaag gcaccaccgc cgatctgatc ttcagcgtgc
     1141 ccttcctgat cgcctactta agcgaattta tgaccctgaa tccgggcgac atgatcgcca
     1201 ccggcacacc aaaaggctta tctgacgtgg tgcctggcga tgaagtagtg gtggaagtag
     1261 aaggcgtggg ctgcctggtg aaccgaattg tgagtgagga aacagcgaaa tgaaaaaagt
     1321 aaatcattgg atcaacggca aaaatgttgc aggtaacgac tacttccaga ccaccaatcc
     1381 ggcaacgggt gaagtgctgg cggatgtggc ctctggcggt gaagcggaga tcaatcaggc
     1441 ggtagcggca gcgaaagagg cgttcccgaa atgggccaat ctgccgatga aagagcgtgc
     1501 gcgcctgatg cgccgtctgg gcgatctgat cgaccagaac gtgccagaga tcgccgcgat
     1561 ggaaaccgcg gacaccggcc tgccgatcca tcagaccaaa aatgtgttga tcccacgcgc
     1621 ttcccacaac tttgaatttt tcgcggaagt ctgccagcag atgaacggca agacctatcc
     1681 ggttgacgac aagatgctca actacacgct ggtgcagccg gtgggcgttt gtgcgctggt
     1741 atcgccgtgg aacgtaccgt ttatgaccgc cacatggaag gtcgcgccgt gtctggcgct
     1801 gggcaatact gcggtgctga agatgtcaga actctccccg ctgaccgctg accgcctggg
     1861 tgagctggcg ctggaagccg gtattccggc gggcgtgctg aacgtggtac agggctacgg
     1921 cgcaaccgca ggggatgcgc tggtccgtca tcatgacgtg cgtgccgtgt cgttcaccgg
     1981 cggtaccgcc accgggcgca acatcatgaa aaacgccggg ctgaaaaaat actccatgga
     2041 actgggcgga aaatcgccag tgctgatttt tgaagatgcc gatattgaac gcgcgctgga
     2101 cgccgccctg ttcaccatct tctcgatcaa cggcgagcgc tgcaccgccg gttcgcgcat
     2161 ctttattcag caaagcatct acccggaatt cgttaaacgc tttgccgaac gcgccaaccg
     2221 tctgcgcgtg ggcgatccga acgatccgaa tacccaggtt ggggcactta tcagccagca
     2281 acactgggaa aaagtctccg gctatatccg tctcggcatt gaagaaggcg caaccctgct
     2341 ggcgggcggc ccggataaac cgtctgacct gcctgcgcac ctgaaaggcg gcaacttcct
     2401 gcgcccaacg gtgctggcag acgtagataa ccgtatgcgc gtcgcccagg aagagatttt
     2461 cgggccggtc gcctgcctgc tgccgtttaa agacgaagcc gaaggcttac gcctggcaaa
     2521 cgacgtggag tacggcctcg cgtcgtacat ctggacacag gatgtcagca aagtgttacg
     2581 cctggcgcgt ggcattgaag caggcatggt gttcgtcaat acccagaacg tccgcgacct
     2641 gcgccagccg tttggcggcg taaaagcctc cggcaccggg cgtgaaggcg gtgagtacag
     2701 cttcgaagtg ttcgcggaaa tgaagaacgt ctgcatttcc atgggcgacc atccaattcc
     2761 gaaatgggga gtctgatatg ggtaagttag cgttagccgc caagatcacg cacgtaccgt
     2821 cgatgtatct ctctgaacta ccaggaaaaa accacggttg tcgccagggt gcgatcgacg
     2881 ggcataaaga gatcagcaag cgttgccggg aaatgggcgt cgataccatt atcgttttcg
     2941 acacccactg gctggtcaac agtgcttatc acatcaactg tgcagaccat tttgaaggcg
     3001 tctacaccag taacgaactg ccgcatttta ttcgtgacat gacctacaac tacgagggca
     3061 acccggagtt ggggcagcta attgccgatg aagccttaaa gctcggcgtg cgggcaaaag
     3121 cgcacaatat tcccagcctg aaactggaat acggcacgct ggtgccgatg cgctacatga
     3181 atgaagacaa gcacttcaaa gtggtctcca tttcggcttt ctgcacggtt cacgattttg
     3241 ccgacagccg caagctgggc gaagcgattc tgaaagcgat cgaacagtac gacggcaccg
     3301 tggcggtcct tgccagcggt tcgttatcgc accgctttat tgacgatcag cgtgcggaag
     3361 aagggatgaa cagctacacc cgcgagttcg accgccagat ggacgagcgc gtggtgaagc
     3421 tgtggcgcga aggccagttc aaagagtttt gcaatatgct gccggagtac gccgactact
     3481 gctacggcga aggcaatatg cacgacacgg tgatgctgct ggggatgctc ggctgggata
     3541 aatacgacgg caaggtggag tttatcaccg agctattccc aagctctggc accggtcagg
     3601 ttaacgctgt tttcccgctt cccgcgtaag gacgttttat gccgcacttt atcgttgaat
     3661 gcagtgataa catccgcgaa gaagccgacc tgccggggtt gttcgccaaa gtgaatccga
     3721 cgctggcagc cacgggtatt tttccgctgg cgggtattcg cagccgcgtg cattgggtcg
     3781 atacctggca gatggccgac gggcagcatg attatgcctt cgtgcatatg acgttgaaaa
     3841 tcggcgcagg tcgcagcctg gaaagccgcc agcaggcggg tgaaatgctg tttgaactga
     3901 ttaaaacgca cttcgccgcc ctgatggaga gccgcctgct ggcgttgtcg tttgagattg
     3961 aagagctgca tccgacgctg aattttaaac aaaacaacgt gcacgcattg tttaagtagc
     4021 gcgcagattg cccggtggcg ctgcgcttac cgggcctaca aaaccccaaa ccgtacaccg
     4081 taggccggat aaggcgcagc cgcatccggc aatgccacag gatatcgcta tgttcgataa
     4141 acacacccac accctgatcg cccagcgtct ggatcaggca gaaaaacagc gcgaacagat
     4201 ccgcgcgatc tcgctggatt acccggagat caccatcgaa gacgcttacg cggtgcagcg
     4261 tgaatgggtt cgcctgaaaa tcgccgaagg tcgcacgctg aaaggccaca aaatcggcct
     4321 gacctcgaaa gcgatgcagg caagctcgca gatcagcgaa ccggattacg gcgcgctgct
     4381 ggatgacatg ttcttccacg atggcagcga tatcccgacc gatcgcttta tcgtgccgcg
     4441 cattgaagtg gagctggctt tcgtgctggc aaaaccgctg cgtgggccaa actgcacgct
     4501 gttcgacgtc tacaacgcca cggactacgt gatcccggcg ctggagctga tcgacgctcg
     4561 ctgccacaac atcgatccgg aaacccagcg cccgcgcaaa gttttcgaca ccatttctga
     4621 taacgccgcc aacgccgggg tgatcctcgg aggtcgtccg atcaaaccag acgaactgga
     4681 tctgcgttgg atctccgccc tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc
     4741 cgctggcgtg ctgaatcatc cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc
     4801 gtatgacgta caactggaag ccgggcaaat cattctcggt ggttcgttca cccgcccggt
     4861 tccggcgcgt aagggcgaca ccttccacgt cgattacggc aacatgggtt ccattagctg
     4921 ccgctttgtt taaggagaga acgatggaaa acagctttaa agcggcgctg aaagcaggcc
     4981 gtccgcagat cggattatgg ctggggctga gtagcagcta cagcgcagag ttactggccg
     5041 gagcaggatt cgactggttg ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg
     5101 tgctcaccca gctacaggcg attgcgccct accccagcca gccggtagta cgtccgtcgt
     5161 ggaacgatcc ggtgcaaatc aaacaactgc tggacgtcgg cacacaaacc ttgctggtgc
     5221 cgatggtaca aaacgctgac gaagcccgtg aagcggtacg cgccacccgt tatccccccg
     5281 ccggtattcg cggtgtgggc agtgcgctgg cccgagcctc gcgctggaat cgcattcctg
     5341 attacctgca aaaagccaac gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg
     5401 caatgaagaa cttaccgcag attctggacg tggaaggggt cgacggcgtg tttatcggcc
     5461 cggcggatct gagcgccgat atgggttatg ccggtaatcc tcagcacccg gaagtacagg
     5521 ccgcgattga gcaggcgatc gtgcagatcc gcgaagcggg caaagcgccg gggatcctga
     5581 tcgccaatga gcaactggca aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg
     5641 gcgttgacac caccctgctc gcccgcgccg ccgaagcgct ggcagcacgg tttggtgcgc
     5701 aggctacagc gattaagccc gacgtgtatt aatgccggat gcgctgcgct tatccggcct
     5761 acactcgcac caaaccgtag gccggataag gcgtttacgc cgcatccggc aaaaagccgt
     5821 accaaatcgc ggaccggata aggcgtttgt gcgcatccgc cataaacctt gtcgtaccct
     5881 acaaaaatcc cattagagga agtaaaatga gcgacacctc acctgccata ccggagagta
     5941 tcgatccggc gaatcagcat aaagcgctga ctgccggaca acaggcggtt attaagaagc
     6001 tatttcgccg cctgatcgtc tttctgttcg tgctgtttat cttctcgttc cttgatcgca
     6061 tcaacatcgg ctttgccgga ctcacgatgg gacgcgacct cggtctgagc gccaccatgt
     6121 ttggcctcgc taccaccttg ttctacgccg cttacgtcat cttcggcatt cccagcaaca
     6181 ttatgctgag tattgtcggt gcacggcgct ggatcgccac catcatggtg ctttggggca
     6241 tcgcctctac tgccaccatg tttgccactg gccccaccag cttatacgta ctgcgtatac
     6301 tggttggcat taccgaagcc ggctttctgc ctggcattct gctgtattta accttctggt
     6361 ttccggccta tttccgcgcc cgtgccaacg ccttgtttat ggtagcaatg ccggtaacga
     6421 cagcgttggg atcgatcgtt tccggctaca ttttgtcgct ggatggcgta atggcattaa
     6481 aaggctggca gtggctgttt ttgctggaag gcttcccgtc ggtattactc ggcgtcatgg
     6541 tgtggttctg gcttgatgac tcaccggaca aagctaagtg gctgacgaaa gaagacaaaa
     6601 aatgcctgca agagatgatg gataacgatc gtctgacgct ggttcagcca gagggagcca
     6661 tcagccacca cgccatgcaa caacgcagta tgtggcggga gatcttcact ccggtggtga
     6721 tgatgtatac cctggcgtat ttctgcctga ccaacacact tagtgcgatc agcatctgga
     6781 caccgcagat cctgcaaagc tttaatcagg gcagcagtaa tatcaccatc ggcctgctgg
     6841 ccgccgtacc gcagatttgt accattctcg ggatgatcta ctggagccgt cactcagatc
     6901 gccgccagga acgaaggcat cacaccgccc ttccttattt gttcgctgcc gctggttggt
     6961 tactggcttc ggcaactgat cacaacatga tccagatgct ggggatcatt atggcttcga
     7021 ccggatcatt cagcgcaatg gcgattttct ggacaacacc ggatcagtcc atcagcctgc
     7081 gggcacgagc gatcggtatt gcggtgatca acgccactgg caacattggt tcagcgttaa
     7141 gtccgtttat gatcggctgg ttgaaagatc tgaccggcag ctttaacagt ggattgtggt
     7201 ttgttgccac gctgctggtg attggtgcgg ggattatctg ggcgattcca atgcagtcct
     7261 cccgtccgcg agcgaccccg taaggaacga cgatgtgtga ccgtcagatt gccaatattg
     7321 atatcagcaa agagtacgat gaaagcctgg gcacggacga tgtgcattat cagtcgttcg
     7381 cccgcatggc ggcctttttt ggccgccata tgctgccaca tcgccacgaa cagtactttc
     7441 agatgcattt cctcaatagc ggacagattg agctacagct tgacgatcat cgctactcgg
     7501 tggaagcgcc cctgtttgtc ctgacgccgc cgtcagtacc tcatgcgttt attacggagt
     7561 ctgatgctga cggtcatgta ttgacggtac gggaagatct gatctggccc ctgctggaag
     7621 ttctttatcc gggcactcgg gaaaccttcg gcctgccggg gatttgcctg tcactggcag
     7681 ataaacccga cgaactggcg gcgctggaac actattggca actgatagag cgggaatcgg
     7741 tagaacaact gcctggtcgg gaacacaccc tgacgttact ggcacaggca gtgttcaccc
     7801 tactgctgcg taacgcaaaa ctcgacgacc atgccgccag cggaatgcgc ggagaattaa
     7861 aactgttcca gcgttttcat atgctgattg aaagccattt tcatcagcac tggacagtac
     7921 cggattacgc taacgaattg catatcaccg aatcacgcct cactgacatc tgccgccgct
     7981 ttgccaaccg tccgccaaaa cggttgattt tcgacaggca gctgcgagaa gccaagcggc
     8041 tgctgctgtt ttctgataac gccgtgaaca atattgcctg gcaactcggt tttaaggatc
     8101 cggcttattt tgcgcgcttt tttaatcgct tagtcggttg ctcgcccagt gcttatcgcg
     8161 ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa cgggaaacca ggcaccacct
     8221 gctattcccc tttctccgcc tggagcaagg ggatttagta acgttaaaaa tcgctaaaag
     8281 cgacctcgat cacaaaaccg tacccgatct gaaaagtacc agccattgtc ccaaaagtgt
     8341 cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa aaacaacaca caaaattaac
     8401 aatcgtattc cgattaatac tgtagaggtc gacatgaaac cagaagattt ccgcgccagt
     8461 acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg tcgcgagatc
     8521 tatatctatg gcgagcgagt gaaagacgtc accactcatc cggcatttcg taatgcggca
     8581 gcgtctgttg cccaactgta cgacgcgcta cacaaaccgg agatgcagga ctctctgtgc
     8641 tggaacaccg acaccggtag cggcggctat acccataaat tcttccgcgt ggcgaaaagt
     8701 gccgacgacc tgcgccagca acgcgacgcc atcgctgagt ggtcacgcct gagctatggc
     8761 tggatgggcc gtaccccaga ctacaaagcc gctttcggtt gcgcactggg cgcgaatccg
     8821 ggcttttacg gtcagttcga gcagaacgcc cgtaactggt atacccgtat tcaggaaact
     8881 ggcctctact ttaaccacgc gattgttaac ccaccgatcg atcgtcattt gccgaccgat
     8941 aaagtgaaag acgtttacat caagctggaa aaagagactg acgccgggat tatcgtcagc
     9001 ggtgcgaaag tggttgccac caactcggcg ctgactcact acaacatgat tggcttcggc
     9061 tcggcacaag tgatgggcga aaacccggac ttcgcactga tgttcgttgc gccaatggat
     9121 gccgatggcg tgaaattaat ctcccgcgcc tcttatgaga tggtcgcggg tgctaccggc
     9181 tcgccgtacg actacccgct ctccagccgc tttgatgaga atgatgcgat tctggtgatg
     9241 gataacgtgc tgatcccatg ggaaaacgta ctgatctacc gcgatttcga tcgctgccgt
     9301 cgctggacga tggaaggcgg ttttgcccgt atgtatccgc tgcaagcctg tgtgcgcctg
     9361 gcagtgaaac tagacttcat tacggcactg ctgaaaaaat cactcgaatg taccggcacc
     9421 ctggagttcc gtggtgtgca ggccgatctc ggtgaagtgg tggcgtggcg caacaccttc
     9481 tgggcattga gtgactcgat gtgttcagaa gcgacaccat gggtcaacgg tgcttattta
     9541 ccggatcatg ccgcactgca aacctatcgc gtactggcac caatggccta cgcgaagatc
     9601 aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct atctcccttc cagtgcccgt
     9661 gacctgaaca atccgcagat cgaccagtat ctggcgaagt atgtgcgcgg ttcgaacggt
     9721 atggatcacg tccagcgcat caagatcctc aaactgatgt gggacgccat tggcagcgag
     9781 tttggtggtc gtcacgaact gtatgaaatc aactactccg gtagccagga tgagattcgc
     9841 ctgcagtgtc tgcgccaggc acaaagctcc ggcaatatgg acaagatgat ggcgatggtt
     9901 gatcgctgcc tgtcggaata cgaccagaac ggctggactg tgccgcacct gcacaacaac
     9961 gacgatatca acatgctgga taagctgctg aaataacgca gcaggaggtt aagatgcaat
    10021 tagatgaaca acgcctgcgc tttcgtgacg caatggccag cctgtcggca gcggtaaata
    10081 ttatcaccac cgagggcgac gccggacaat gcggaattac ggcaacggcc gtctgctcga
    10141 tcacggatac accaccgtcg ctgatggtgt gcattaacgc caacagtgcg atgaacccgg
    10201 tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa tcatgagcag gagctgatgg
    10261 cccgccactt cgcgggcatg acaggcatgg cgatggaaga gcgttttagc ctctcatgct
    10321 ggcaaaaagg tccgctggca cagccggtgc tgaaaggttc gctggccagt cttgaaggtg
    10381 agatccgcga tgtgcaggca attggcacac atctggtgta tctggtggag attaaaaaca
    10441 tcatcctcag tgcagaaggt cacggactta tctactttaa acgccgtttc catccggtga
    10501 tgctggaaat ggaagctgcg atttaagctc cagccccttc acgcattcgt cgaaggggca
    10561 tttttattgt gatctcaatc acatatccac ccgatgaata attcttaccc aaccgctcac
    10621 cgccattcct gaccgcttac atccctaaaa taaccactca gttatttacc ttactttacg
    10681 cgtgcgtaac tctggcaaca tcactgcagg atagcggtca atttacctcc tcaaacgcaa
    10741 cgcaaaccta gaacggcttc ggcctatgat taacaaatta ctctatggat tttggtttgc
    10801 aggaaggcag caagtgagtg aatccccggg agcttataac agtaagtgac aggggtgaac
    10861 gaacgcagct gccgcacctg taagccaaaa gacgacgagt aaaatgccag gttttactat
    10921 ggatacgaaa aaactattca agcacatacc ctgggtgatt ctcggaatca tcggtgcatt
    10981 ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc agcgccctgt ggatcgtggt
    11041 cgcctctgtg tcggtgtatc tggtggcgta tcgctactac agtctgtata tcgcccagaa
    11101 ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt attaacaacg acggtctgaa
    11161 ctacgttccg accaaccgtt acgtgttgtt tggtcaccac ttcgccgcta tcgccggtgc
    11221 tggtccgctg gttggtccgg ttctcgccgc gcagatgggc tacctgcccg gcacgctgtg
    11281 gctgctggcg ggggtagtgc tggccggtgc ggtacaggac tttatggtgc tgtttatctc
    11341 ctctcgccgt aacggcgcat ctctcggtga gatgatcaaa gaagagatgg gaccagtacc
    11401 ggggactatc gcgctgtttg gctgtttctt aatcatgatc atcatccttg ccgtcctggc
    11461 gctgattgtg gttaaagccc tggcagaaag tccgtggggt gtcttcaccg tttgctcaac
    11521 cgtaccgatt gcgctgttta tgggtatcta catgcgcttt atccgtccgg ggcgtgtggg
    11581 tgaagtctct gtaatcggta tcgtgctgct ggttgcctct atctacttcg gtggcgtgat
    11641 tgctcacgat ccgtactggg gtccggcact gacctttaaa gacaccacca ttaccttcgc
    11701 gctgattggc tatgcgtttg tttccgcact gctgccggtg tggctgatcc tcgcaccgcg
    11761 cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc ggcctggcgt tgggtatcgt
    11821 ggtgctgaac ccggaactga aaatgcctgc gatgacccag tacattgacg gtactggccc
    11881 gctgtggaaa ggcgctctgt tcccgttcct gttcatcacc atcgcctgtg gtgcggtatc
    11941 tggcttccac gcgctgatct cttccggtac gacgccgaaa ctgctggcta acgaaaccga
    12001 cgcgcgtttc attggctacg gcgcaatgct gatggagtcc ttcgtggcga ttatggcgct
    12061 ggttgcagcg tccatcatcg aaccgggtct ttacttcgcg atgaacaccc cgcctgcggg
    12121 ccttggcatc accatgccta acctgcatga aatgggtggc gagaacgcgc cgatcatcat
    12181 ggcgcagctg aaagacgtta ccgcacacgc ggcggcgacc gtcagctcct ggggcttcgt
    12241 gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt ggtgagcctt ctgtcctgaa
    12301 ccgtgcaggt ggtgcgccga cactggcggt aggtatcgcg cacgtgttcc acaaagtgct
    12361 gccgatggct gacatgggct tctggtatca cttcggtatt ctgttcgaag ccctgttcat
    12421 cctgaccgcg ctggatgcgg gtacccgttc tggccgcttt atgctgcagg acctgctggg
    12481 taacttcatc ccgttcctga aaaaaaccga ctctctggtt gctggtatca tcggtactgc
    12541 gggctgtgtg ggtctgtggg gctacctgct gtatcagggc gtggtcgatc cgctgggcgg
    12601 cgttaagagc ctgtggccgc tgttcggtat ctccaaccag atgctggcag ccgtagcgct
    12661 ggtactgggc accgttgtgc tgattaagat gaagcgcacc caatacatct gggtaactgt
    12721 tgttccggct gtatggctgc ttatctgcac cacctgggcg ctgggcctga aactgttcag
    12781 caccaacccg cagatggaag gcttcttcta catggcaagc cagtacaaag agaagattgc
    12841 taacggtact gacctgacgg cacagcagat tgccaacatg aaccacatcg ttgtgaacaa
    12901 ctacaccaac gcgggcctga gtattctgtt cctgattgtg gtgtacagca tcatcttcta
    12961 cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa cgtactgaca aagaaacacc
    13021 gtacgttcca atcccggaag gcggcgtgaa gatctcttcg caccactaac cggttgccgg
    13081 atgcgttgcg cgtatccggc ctacaaagtg tctatcgtag gccggataaa gcgtttacgc
    13141 cgcatccggc aaatccctag cccggacctt ccgggcttta cgcatatctg gaaggcaaaa
    13201 tgtttggtaa cttaggacag gcaaaaaaat atctcggcca ggcggcgaag atgttgattg
    13261 gcattccaga ctacgacaac tacgtcgagc atatgaagac caaccatccc gacaagccgt
    13321 acatgagcta tgaagaattc ttccgcgaac gccagaacgc gcgctacggc ggcgatggta
    13381 aaggcggtat gcgctgttgt taatggagag agcatgaacc cgattgcagt taccctactc
    13441 accggttttt taggcgcggg caaaaccacc ctgctgcgcc atattcttaa cgaacaacac
    13501 ggctacaaga ttgccgtgat tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg
    13561 attggcgacc gcgccacgca gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc
    13621 tccaacgagc tggaagacgc actactcgac ctgctggata atctcgacaa gggcaatatt
    13681 cagttcgacc gtctggtcat tgaatgcacc ggcatggccg atcccggtcc gattattcag
    13741 acctttttct cccatgagat tttatgccag cgttacctgc tggacggcgt gattgcgctg
    13801 gtggatgcgg tacatgccga tgagcagatg aaccagttca ccatcgccca gtcccaggtt
    13861 ggctacgccg accgtattct gctgaccaaa accgacgtcg caggcgaagc agaaaaactg
    13921 cgtgaacgcc tggcgcgcat caacgcccgt gcaccggtct acaccgtcac ccacggcgac
    13981 atcgacctgg gcctgctatt caacaccaac ggttttatgc tggaagagaa cgtcgtcagc
    14041 accaaaccgc gcttccactt tatcgcggat aaacaaaacg atatttcgtc gattgtggtg
    14101 gaactggatt acccggtaga tatcagcgaa gtttcccggg tgatggaaaa cctgctgctg
    14161 gaatcggcag ataaactgct gcgttacaaa gggatgctgt ggattgacgg cgaacctaac
    14221 cgcctgctat tccagggcgt ccagcgcctc tacagcgccg actgggaccg cccgtggggc
    14281 gatgaaaaac cgcatagcac gatggtgttt atcgggattc agctgccgga ggaagagatt
    14341 cgggcggcgt ttgcggggtt gaggaagtaa tcgtgaaagg ttgagtgtaa agcagctcaa
    14401 tgccccattt agcagtgagg ggggaaacag aacgacgtgc tcccctccgc taagttttat
    14461 tgcgcgtgaa tacctggatc tggcaaaaaa ttgccgcgtt tggcgtcaga ccatgcttcg
    14521 ctaccagaag caggaatgtc gctgtaatca atgtcttcgt cggatttatt aaccaaagcc
    14581 atgagttcag cttcttgctg cacatcaagt gtgtagagtg tgccccgttt atgtttaacc
    14641 atgctcattt atgaccagaa cctttacgat ttaattataa agatgactta tttcagccat
    14701 ccaggcacgt tatattctga gacattctca atggaatatg catctctcct gtcagaacct
    14761 ttcttacact tcaactcatt gaccccccat ttaccctctc tattggaata aaatacagta
    14821 tatttatcgg tattaaaccc gaaatatctt tcatccggac gtgaaatatt caactctcca
    14881 catttaacct gattacgcca ttcaaaatct atggtgcttt ttaaaacgta gtacgaaata
    14941 ccttctacat tttttgaagc aaaagccact aaaatagtag atacgataaa tgctccggca
    15001 agtataaaca acctatatac acttaattta gcccacagag ttaaaaaagc aaaatagata
    15061 acaagttgca ttaacataaa gaaaagcaac ataaaagcca tccctttcca gaaatactca
    15121 acccatggcg ttagtgtgat attgagatta ctcaaactta aaaacaaact ggaagcacaa
    15181 ctacttgcaa acaaatatat aattggtatg caagctataa tcatggacct aataagtatt
    15241 agcttattat tgttttcaaa ttccaattct aatataacaa aaatacttcc cagaaaaacg
    15301 aatactgaaa aagcactaaa aacgtataga gaggcactga ctgaaaaaat at
//
LOCUS       DAZGOB010000119        15296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGOB010000119 DAZGOB010000000
VERSION     DAZGOB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.119"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(225..1049)
                     /gene="iclR"
                     /locus_tag="ACHLIF_003892"
     CDS             complement(225..1049)
                     /gene="iclR"
                     /locus_tag="ACHLIF_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO9607814.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1249..4932
                     /gene="metH"
                     /locus_tag="ACHLIF_003893"
     CDS             1249..4932
                     /gene="metH"
                     /locus_tag="ACHLIF_003893"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO9607815.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYCESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5152..6783
                     /locus_tag="ACHLIF_003894"
     CDS             5152..6783
                     /locus_tag="ACHLIF_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO9607816.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6874..7563)
                     /gene="pepE"
                     /locus_tag="ACHLIF_003895"
     CDS             complement(6874..7563)
                     /gene="pepE"
                     /locus_tag="ACHLIF_003895"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO9607817.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7946..9175)
                     /gene="sorE"
                     /locus_tag="ACHLIF_003896"
     CDS             complement(7946..9175)
                     /gene="sorE"
                     /locus_tag="ACHLIF_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO9607818.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            complement(9227..10050)
                     /locus_tag="ACHLIF_003897"
                     /pseudo
     CDS             complement(9227..10050)
                     /locus_tag="ACHLIF_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(10061..10858)
                     /locus_tag="ACHLIF_003898"
     CDS             complement(10061..10858)
                     /locus_tag="ACHLIF_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO9607819.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            complement(10924..11418)
                     /locus_tag="ACHLIF_003899"
     CDS             complement(10924..11418)
                     /locus_tag="ACHLIF_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO9607820.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11418..11825)
                     /locus_tag="ACHLIF_003900"
     CDS             complement(11418..11825)
                     /locus_tag="ACHLIF_003900"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO9607821.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            complement(11835..12641)
                     /locus_tag="ACHLIF_003901"
     CDS             complement(11835..12641)
                     /locus_tag="ACHLIF_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9607822.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(12711..13658)
                     /locus_tag="ACHLIF_003902"
     CDS             complement(12711..13658)
                     /locus_tag="ACHLIF_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO9607823.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            14006..14878
                     /gene="rluF"
                     /locus_tag="ACHLIF_003903"
     CDS             14006..14878
                     /gene="rluF"
                     /locus_tag="ACHLIF_003903"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO9607824.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(14879..15151)
                     /locus_tag="ACHLIF_003904"
     CDS             complement(14879..15151)
                     /locus_tag="ACHLIF_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO9607825.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
BASE COUNT         3632 a         4000 c         4019 g         3645 t
ORIGIN      
        1 tcgctcaatc tcataatgca gccgtaaaag tttcggtgga atgagatctt gcgattttgt
       61 taatgattaa gtagtttgat gtttaaaaat aatgctgagt taataaaagt gtgtgaagtg
      121 tatgacatta agttattttg gcgtcaatgc gattaacaga cacccttatt ctattgccac
      181 tcaggtatga tgggcagaat attgcctctg cccgccagaa aaagtcagcg cattccaccg
      241 tacgccagcg tcacttcctt cgccgcttta atcaccatcg cgccaaactc ggtcacgcgg
      301 tcatcggtaa tacgtgaaat cggtccggaa attgaaattg cggcaaacgg ttcgcggtgc
      361 tcatcgaaaa tacacgctgc aaggcaacgt agccccagtg catgttcctc atcgtcaaat
      421 gaataacccc gtttgcgcgt ttgggcgaga tcttctttta aatgcacagg agacaccagc
      481 gttgcgtggg tataggcatg taaccctttg cggtgcagca gcttcgtcac ctgttcttcg
      541 ctcagttggg ctaaaaaggc tttacccgca ccggaagcgt gcatcggcaa tttaccgccg
      601 ataggcgcgg acattcgcat cagatgcgta cactgtacct ggtcgataat aatcgcttcg
      661 tgatcgcttt gatcaagcac cgccatattg accgtttcgc cagactcttc cattaaattg
      721 cgcaggatag ggtgaacaat cgctaacaaa ttacggctct ggagaaagct gctgccgacc
      781 ataaaggcat gtgcgccgat tgcccaatgt cccagttcgc cgacctgacg cacgaaaccc
      841 tgctgttgca tcgtggttag caggcggtgg gtcgtggaat tgggtaaccc ggcttgttgc
      901 gccagctctg tgagtgccac actgccattg gattcggcaa tccactccag taatttcagg
      961 ccacgcgtta aagactgaac ctgtccagtc gctggtgcgg tggcaacggc gggttttctg
     1021 ccgcgtttcg cgggaatggg tgcgaccatg acagcctcct ttttctgtat cgtggaaatc
     1081 attttcattt ttattgttag ctaatgcaat agttgctgaa ctgatccgat gagttaatgt
     1141 tgaacaaatc tcatgttgcg tggtggtcgc ttttaccaca gatgcgttta tgccagtatg
     1201 gtttgttgaa tttttattaa atctgggttg agcgtgtcgg gagcaagtgt gagcagcaaa
     1261 gtggaacaac tgcgtgcgca gttaaatgaa cgtattctgg tgctggacgg cggtatgggc
     1321 accatgatcc agagctatcg actgaacgaa gccgattttc gtggtgaacg ctttgccgac
     1381 tggccatgcg atctcaaagg caacaacgac ctgctggtgc tcagcaaacc ggaagtgatc
     1441 gccgctatcc acaacgccta ctttgaagcg ggcgcggata tcatcgaaac caacaccttc
     1501 aactccacga ccattgcgat ggcggattac cagatggaat ccctgtcggc ggaaatcaac
     1561 tttgcggcgg cgaaactggc gcgcgcctgt gctgacgagt ggaccgcgcg cacgccggag
     1621 aaaccgcgct acgttgccgg tgttctcggc ccaaccaacc gcacggcgtc tatttctccg
     1681 gacgtcaacg atccggcatt ccgcaacatc acttttgacc agctggtggc ggcctattgc
     1741 gaatccacca aagcgctggt ggaaggtggt gcggatctga tcctgattga aaccgttttc
     1801 gacaccctta acgccaaagc ggcggttttt gcggtgaaaa cggagtttga agcgctgggc
     1861 gttgagctgc cgattatgat ctccggcacc atcaccgacg cctccgggcg cacgctctcc
     1921 gggcagacca ccgaagcatt ttacaactca ttgcgccacg ccgaagctct gacctttggc
     1981 ctgaactgtg cgctggggcc cgatgaactg cgccagtacg tgcaggagct gtcacggatt
     2041 gcggaatgct acgtcaccgc gcacccgaac gccgggctac ccaacgcctt tggtgagtac
     2101 gatctcgacg ccgacacgat ggcaaaacag atacgtgaat gggcgcaagc gggttttctc
     2161 aatatcgtcg gcggctgctg tggcaccacg ccacaacata ttgcagcgat gagtcgtgca
     2221 gtagaaggat tagcgccgcg caaactgccg gaaatccccg tagcctgccg tttgtccggc
     2281 ctggagccgc tgaacattgg cgaagatagc ctgtttgtga acgtgggtga acgcaccaac
     2341 gtcaccggtt ccgctaagtt caagcgcctg atcaaagaag agaaatacag cgaggcactg
     2401 gatgtggccc gccagcaggt ggaaaacggt gcgcagatta tcgatatcaa catggatgaa
     2461 gggatgctcg acgccgaagc ggcgatggtg cgttttctca atctgattgc cggtgaaccg
     2521 gatatcgctc gcgtgccgat tatgatcgac tcctcaaaat gggatgtcat tgaaaaaggt
     2581 ctgaagtgta tccagggcaa aggcattgtt aactctatct cgatgaaaga gggcgtcgac
     2641 gcctttatcc atcacgcgaa gctcttgcgt cgttacggtg cggcagtggt ggtaatggcc
     2701 tttgacgaac agggacaggc cgatacccgc gcacggaaaa tcgagatttg ccgtcgggcg
     2761 tacaaaatcc tcaccgaaga ggttggcttc ccgccagaag atatcatctt cgacccgaac
     2821 atcttcgcgg ttgccaccgg cattgaagag cacaacaact acgcgcagga ctttatcggc
     2881 gcgtgtgaag acatcaaacg cgaactgccg cacgcgctaa tttccggcgg cgtatctaac
     2941 gtttctttct cgttccgtgg caacgatccg gtgcgcgaag ccattcacgc agtgttcctc
     3001 tactacgcta ttcgcaatgg catggatatg gggatcgtca atgccgggca gctggcgatt
     3061 tacgacgacc tgcccgctga actgcgcgac gcggtggaag atgtgattct taatcgtcgc
     3121 gatgatggca ctgagcgttt actggagctt gccgagaaat atcgcggcag caaaaccgac
     3181 gacaccgcta acgcccagca ggcggagtgg cgctcgtggg aagtgaataa acgtctggaa
     3241 tactcgctgg tcaaaggcat taccgagttt atcgagcagg ataccgaaga agcccgccag
     3301 caggctacgc gcccgattga agtgattgaa ggcccgttga tggacggcat gaacgtggtc
     3361 ggcgatctgt ttggcgaagg gaaaatgttc ctgccacagg tggtcaaatc ggcgcgcgtc
     3421 atgaaacagg cggtggcgta tctcgaaccg tttattgaag ccagcaaaga gcagggcaaa
     3481 accaacggca agatggtgat cgccaccgtg aagggcgacg tccacgacat cggtaaaaat
     3541 atcgttggtg tggtgctgca atgtaacaac tacgaaattg tcgatctcgg cgttatggtg
     3601 cctgcggaaa aaattctccg taccgctaaa gaagtgaatg ctgatctgat tggactttcg
     3661 gggcttatca cgccgtcgct ggacgagatg gttaacgtgg cgaaagagat ggagcgtcag
     3721 ggcttcacca tcccgctatt gattggaggc gcgacgacct caaaagcgca cacggcggtg
     3781 aaaattgagc agaactacag cggcccgacg gtgtatgtac agaacgcatc gcgcaccgtt
     3841 ggtgtggtgg cggcgctgct ttccgatact cagcgtgatg attttgtcgc tcgtacccgc
     3901 aaggagtacg aaaccgtacg tattcagcac gggcgtaaga aaccgcgcac accaccggtc
     3961 acgctggaag cggcgcgcga taacgatttc gcttttgact ggcaggctta cacgccgccg
     4021 gtggcgcacc gtctcggcgt gcaggaagtt gaagccagca tcgaaactct gcgtaattac
     4081 atcgactgga caccgttctt tatgacctgg tcgctggccg ggaagtatcc gcgcattctg
     4141 gaagatgaag tggtgggcgt tgaggcgcag cggctgttta aagacgccaa cgacatgctg
     4201 gataaattaa gcgccgagaa aacgctgaat ccgcgtggcg tggtgggcct gttcccggca
     4261 aaccgtgtgg gcgatgacat tgaaatctac cgtgacgaaa cgcgcaccca tgtgatcaac
     4321 gtcagccacc atctgcgtca acagaccgaa aaaacgggat tcgctaacta ctgtctcgct
     4381 gacttcgttg cgccgaagct ttctggtaaa gcagattaca tcggcgcatt tgccgtgact
     4441 ggcgggctgg aagaggacgc actggctgat gcctttgaag cgcagcacga tgattacaac
     4501 aaaatcatgg tgaaagcgct tgccgaccgt ttagccgaag cctttgcgga gtatctccat
     4561 gagcgtgtgc gtaaagtcta ctggggctat gcgccgaacg agaacctcag caacgaagaa
     4621 ctgatccgcg aaaactacca gggcatccgt ccggcaccgg gctacccggc ctgcccggaa
     4681 catacggaaa aagccaccat ctgggagctg ctggaagtgg aaaaacatac cggcatgaaa
     4741 ctcacagaat ctttcgccat gtggcccggt gcatcggttt cgggttggta tttcagccac
     4801 ccggacagca agtactacgc ggtggcgcaa attcagcgcg atcaggttga agactatgcc
     4861 cgccgtaaag gtatgagcgt ctccgatgtt gagcgctggc tggcaccgaa tctggggtat
     4921 gacgcggact gattcacaaa tctgtcactt ttccttacaa caaacagggc gctcaatgag
     4981 tgccctgtct ctttattaat atgaaacact tatactggaa acaggctgga ataatcttag
     5041 ccggaaaaac gaatgtcaaa aggcacagta aagatgccag tcagcgtgtt tccggtcctt
     5101 tggtcattca attgcttcag gaacatataa cgataaggag aacctgaact cgtgttaacg
     5161 ctgcttcacc tgctttctgc cgtcgccctg ctggtctggg ggactcatat tgttcgaacc
     5221 ggcgtaatgc gcgtcttcgg cgcgcgtttg cgtactgtcc ttagccggag cgtcgaaaaa
     5281 aagccgctcg ccttttgcgc ggggatcggc gttaccgcac tggtgcagag cagtaatgcc
     5341 accaccatgc tggtgacctc gtttgtcgct caggatctgg tagccctcgc accggctctg
     5401 gtcattgtgc tgggggcaga tgtcgggacg gcgctaatgg cgcgtattct caccttcgac
     5461 ttatcctggc tgtcaccgtt acttattttt atcggcgtga tttttttcct cggacgcaaa
     5521 cagtcacgcg ccgggcaact gggccgcgtc ggtattggtc ttgggctgat tttactggcg
     5581 ctggagttga ttgtgcaggc cgtaacgccg atcacccagg caaacggcgt tcaggtgatc
     5641 tttgcctcgc tgaccggcga tattctgctg gatgcgctga ttggcgcgat gttcgccatt
     5701 atcagctact ccagccttgc tgctgtactg ctgactgcga ctctgaccgc cgcaggcatt
     5761 atctccttcc ccgtggcgct ctgtctggtg attggtgcta acctcggttc cggcctgctg
     5821 gcgatgctca acaacagtgc cgccaatgcc gcagcccgcc gtgtcgcgct gggtagtctg
     5881 ctgtttaagc tggtgggtag cctgattatc ctgccgtttg tccatttgct ggcagagaca
     5941 atggggaagc tgtcattgcc aaaagcggaa ctggtgatct atttccacgt cttctacaac
     6001 cttgtacgtt gcctggtcat gctgccattt gttgacccga tggcacggtt ttgcaaaacg
     6061 attattcgcg atgaaccgga actggatacc cagctacggc ctaaacatct ggatgtcagc
     6121 gcgctggata cgcccacgct tgctctggcg aacgccgcgc gcgaaaccct gcgcattggc
     6181 gacgccatgg aacagatgat ggaagggctg aataaagtga tgcacggcga gccacggcag
     6241 gagaaagagc tgcgtaagct ggcagatgat atcaacgttc tctataccgc cattaagctg
     6301 tatctggcgc ggatgccaaa agaggaactg gcagaagaag agtcgcgtcg ctgggcggag
     6361 attattgaaa tgtcgctcaa ccttgaacag gcttccgata tcgtcgagcg catgggcagc
     6421 gaaattgccg ataaatcgct ggcagcacgg cgggcatttt cgcttgatgg gttgaaggaa
     6481 ctggatgcgc tctatgagca attgctcagt aatttaaagc tggcaatgtc ggtgttcttc
     6541 tctggcgatg tcaccagcgc tcgtcgtttg cgtcgcagca aacatcgttt tcgcattctt
     6601 aatcgccgct attcccacgc ccacgtcgat cgcctgcatc agcaaaacgt gcagagcatt
     6661 gaaaccagtt cgctacattt aggcttactg ggagatatgc agcgcctgaa ctcgctgttt
     6721 tgttcggtgg cttacagtgt gctggaacag ccggatgaag atgaaggacg ggacgagtat
     6781 taatattgtg aatcctgtag gcctgataag acgcgtcaag cgtcgcatca ggcactatga
     6841 acaagatgcc ggatcatgca tccggcaatt tacttaaaaa cggtgaccag cttctagcgg
     6901 caccgcttct tcaccagcct taaacacata agtggtgttc gggccaccca gcgtagcgtg
     6961 acctttactc actgtgatcc agttaccttc cggtagacca ataatcgtca gttctggcgc
     7021 gacgaccagc agttcgcgaa tacgctgctc acgggtttca cctttatggc cttccggcag
     7081 cgcgttggtg aagtgcgggt tgatttgcag cgggaacaga tttagcgcat cgaaaccttg
     7141 cggatcgaca atcggcatat cgttggtggt acgaatggtt gggcaagcaa ggttagcgcc
     7201 tgcgctccag ccaatataca gcgcaccacg tttaaccacg tcagtaattg gtgccagcag
     7261 cccgcgctcg cggcactgtt tcagcaactg gaaagtattc ccgcccccga caatcacgat
     7321 ctcagcattt tcaatcgcgg caacgggatc gacaacgcta tgaataccgg tgacagatac
     7381 acccagcgga gcgagaaccg cagccgtttt cgctgtgtaa tcatcccagg tctgcgttac
     7441 gccagcgaaa ggaataaata ccgctgaacg gcgaccctgc aactgttcag caatcagcgg
     7501 cagcgcatgt tccagccagg ctttacccgg cagcgtcgag ttactcaata aaagcagttc
     7561 cattacttct ccagttagta agaaatcacg ggaggcatgc taccactgcg aatttaacca
     7621 cttactgaca tggctcacga tgtcacgtat tgcaccgggc gctgagactg atgacaaaca
     7681 cagcattgcc tgatgcgcta cgcttaccag gcctaccaga taactgcaat atattgaatt
     7741 ttcgagatta tgtaggccga ataaggcttt attccgcatc cggcataaac aatgtgtact
     7801 ttgccaccaa tttgagcgcc ccttaaggcg ctgatgtttt tttatccttc tcgtgcacat
     7861 tgggccgctt cagcaatcag ccacacatca ggtcgcgaag ttgccgcggc gaccagccag
     7921 cgacatgcag agttaagtag cgcgattaat catgggaaat tgcctctgca tgagtgagca
     7981 gatattgctc cgcctcaccg gaccagatcc cctgatgacg cgccagaatc gccgccagtg
     8041 cttgatcctg aatctgcgtg agtgacgtca gcggcaggta tttcccggta tacaccagct
     8101 ttttgccgcc gccgacggca ggcaattcaa gcgtggtttc gcccgcggca ttcagcccaa
     8161 gaatatgtgt taccactttt gcggcctgca cttttttctc ttcaatcagt ttgaccgccg
     8221 cgcgcatgtc gtcggtattg ccgcctgacg tgcccacgta gtgggtaaat gcataatgca
     8281 catcgtagaa attaattggc gcgctgaaat gtttatcctg cggtccggcg aagaaattca
     8341 ggcaaccatc tgtcgccagc aaggaagagg cgagagtcac cagtccttca ttaggcacaa
     8401 agacgaaaat atcatcgaag ccgtgaccgc cactcagcgc cattagcgta tcaaatgctg
     8461 catcggcggc attgagataa tgaatcagtg tttgcggttc tgatggatag tgcttgcgcg
     8521 cataactcaa tttatcgttg tcggtatcgg taatgacgag cagcgacggg ttaacgggtc
     8581 catgtagcgc ataatcaatc gccaacagtc ccattggtcc ggtgccgccg aggatcagca
     8641 tgcgcccttg cgggcgaatc cccatcgtgt ggttataact accttcctga agatgatagt
     8701 tggcgttgaa cgcgccaatc acgcaggaaa gcggttcaac cagtgagcct tcaaaatagg
     8761 tttcgccgtc gtatgccagc aggcaatctt gttccatgac ctcgttggga ataaccacat
     8821 gcgtggcttc gccgcccacc cacgggaagg agtagccggg gcagtccggg cggtcgggga
     8881 gttgcaggtt ggcctgaatc acataacgct gacccggctg gaatttgtgc tgccactttt
     8941 tacccacggc cagaatatcg ccgcaaaact cgtggccgat gatgatgggg ttggtcgcca
     9001 catcgtcggg tactttttta tggttttcac ccagattggc ctctttccag gaagagaggc
     9061 acaggctgtc agtgaccacc gtcgcgagga tttcatcctc ctgcatttca ggaaggtcaa
     9121 aggtttccag gcgtaaatca cgtttaccat aaagacgcag agctgttgtt ttcataaacc
     9181 acctcatgtt gagcgatggc cccggcagtc gtgccggggc gaagaatcag gacaggaagc
     9241 ccagcgcatt accgataatc cctaagccaa acagggcgaa aatcagccat accgggttaa
     9301 tttttttgtt gagcagacga accataagca gcgtcagacc gagcgccagc aaaccagggc
     9361 aaagttggtc gagaatgttc tgcacggtca tggtgacggt ggagccatcg gcggcatgcg
     9421 tttgtgaaac caccaacggt acgttgattg acgtccattt ggtgaccagc acgcccatca
     9481 caaacagtcc gagaatcgac gcgccttcgg tgagtttttg cagcacattc ccgcccatat
     9541 cgctgacgat attcacccct ttgcgaaagc cgagctgtag gccataccac ttcatcgcca
     9601 gacgcaccgc attgaaaata aagaaaaaaa aagcgggccg aggatgttgc cagaaagtgc
     9661 cagagatgcg ccgagcgcag cggtaatcgg gcgcagcgtt ccccaaacca gtggatcgcc
     9721 aacccctgcc aatggtccca tcagaccgac tttgatgccg ttaatggtac cgtcatcaat
     9781 ttccgcgccg ttggcccgcg cctcttccat ggcggcagtg acgccgatga ccgggccaca
     9841 tacggctggc gtggtattga agaacaccag gtgtcgcctg agcgccgcaa cctgatcctc
     9901 ttttaatggg taaagtcgct tgatggcggg gatcatgtcg tagcaaaagc ccagcccgtg
     9961 aatacgttca aagttaaagg acgcctgttg caggttggag cgcagaaaca tgctcaccag
    10021 atcgctgcgt gtaatttttc tctgttccat gatggctccg ttaatcgtca agttggtcaa
    10081 gggcggtgga ggtggtggcc tggggttgag gttcagcttt acgccactgt ggatttagct
    10141 ggatgtagac cagggccatg atcacgccga cgccaccgaa cgccagcaga ctgagatcga
    10201 ggtagccacc tgcgaggaaa ccgaggaaaa agaaaggcat caaatatttc acgcccatca
    10261 tacgtagcac catggcgtaa ccgaccacca cgataaaacc gccagcaatt tgcagaccgc
    10321 gcgtcacgaa ttccggtatc gcgctcagca tattgctcac catatcggcg ctgacgaaca
    10381 gtgagacaat cagtgccgga atagcaacgc gcagcgcctg cacgccaagc gcggagacat
    10441 gcagaatatc gagagtgcga aaccgcgctt cttccgctgc tttatccgcc gcgtgctgga
    10501 acactacggt gatggtacgg gcaaacacag tcagcacctg gcctgccgca gccacaggca
    10561 acgcgatggc gattccggtg gcgatgcttt gctgaccaac gataaccaga atggcggaga
    10621 ttatgctggc gagtgcagaa tccggagatt gcgccgcgcc gacgttcatc cagccgagag
    10681 ctatcaattc cagcgtacca ccgagtatta ttccggtttt taaatcaccg agaattaaac
    10741 caatcaccgt acaggcaatt aacgggcgat gagtctgaaa ttcatccagc acgctgccca
    10801 ttccggcaat acaggaaaaa agaaatatag caatgatttg tagggtactt atttccatga
    10861 tttttcacct tttaagtgtc gtcatccctg cctgttaaag catatgtaca caggcgctat
    10921 tatttaattt gcgattaact gttcgtttat tttgtcgata atattgattg agggatctga
    10981 cgctacgacg cgtaaatcca gtatgacgcc aagattattt aactcatgaa atgcgttaat
    11041 atcatcatca tctaatgaaa ccgctttggt taactgtttt ttaccgggcc gccaggccat
    11101 gccgccaata ttcagggtgt ctatttttac cccctggcga accatcgcta aggcatcctg
    11161 tgggcgagtg aaaagataaa aaacggtttc gtcctgatat tgcgggttgt gataaacagc
    11221 gacggctttt tcaatattga ccacgttaac cttcattccc ggtggcgcag cctgacgcaa
    11281 taatgtccgg cgaacttcat cgttataaac ttcgtcattg cagataataa tgcgctgggc
    11341 gttagccact ttcgaccaga cggtagtgac ctgaccgtgg atcaggcggt catcaatgcg
    11401 ggcgagcgta atgttcatca gaaatcctcc tctgccgttg ccagttgttt ccagacaacg
    11461 caggtttgct ttgcgatttg cgtcaggtgt tcacacagtt catcaacatt catgctgtcc
    11521 tggttatcaa ccagttcgag cgccagcggc agagaaaggc cgctgattac ccgcagacga
    11581 ggattgcgca tcgcaagcat cgctgccgca ttccacgggc tgccgcactg taaatccacg
    11641 gcaatcagcc actcatcttg attgtgaatg cttactaact tttccagctt tgctacgatg
    11701 tctccggcgt tctcgccggg cacaaattca acggcttcaa cattggcgtt gccgtaaacc
    11761 atctgaaccg attccagcat ggcgcaagct aatttgccgt gggcacaaaa aatggcgttg
    11821 accataaagc ctccttagcc gcgcgttttg ccgcccgcaa tgttagtggt tactccggtg
    11881 atgtagctgg cgcgttcaga caacagataa caaacaaaat cagcgacttc tgctaatctt
    11941 ccggcgcgcc caataggaat ggcgtttttg gtatagcctt cacgcagctg ctcgacggtg
    12001 atattgcgcg tccacgccag cgcctcttca tattccggcg tgcgtagccc ggttttttcc
    12061 agaatcccgg gcgcgatacc gaccacacgg ataccgtgtt tacccagctc tttcgaccag
    12121 gagcgcgtga agctattaag cgcggctttg gtagcggcgt aacagctttg gccttctgag
    12181 ccttccagtc cactttctga ggaaacattc acaatcacgc catcatgttg tttgaccatc
    12241 tgtcgcgcca ccgcctgcga catcagaaaa acgcctttct ggttgatatt gaccattttt
    12301 tcgaatgcag cttcgttgag ttcatactgc ccggcaggcg ctttctcatc gaccagcaga
    12361 cgcgggaaat tgaccccggc gttattgacc agaccgtcga tgcgaccaaa acgctggata
    12421 atttccgcta ccgtatgatt tacctctttg gcgctggaaa tatcggtcgg ccaaaactga
    12481 tagcctttat gaccttcata ttgaccatcg ccaccgtgaa tatctaccat ctgtacgttt
    12541 gcgccttgtg ctaataattc ctctacaatc gccagaccaa taccggatgc gccgccggtg
    12601 acgataatga ttttatcctg caaatttaac cacgtttgca taacagactc ctgattttag
    12661 ataataggga cccgccgtaa tttttattac ggtaaacatc gggaagattt ttatttcagt
    12721 aatagttcag ctgtgctgct attcgttacc agacaattaa tatattttcc acgcagtgca
    12781 ccaataattc cgctgtattt ttcttcactc atggcaatgc caatggaata tcgcgcctgc
    12841 tttaatttat tcatttcgat agagagcgtt ttttcgctca tattcgtttc aaccattgcg
    12901 ccgtgaatat caaaaaagcg cgagcaaata tcgccagcaa cctggcgggc attcaggtcg
    12961 tcactctctt caccaccata aaacgcatgc cagttagcgc cgtcgcgaat ggccggtgag
    13021 ccaattccca ccagggcgac atccagatta tcccagtagg cagagatggt tttaaagtgc
    13081 tgagactgca tgatcccatt acgaattaat gggttatcca gcagagccgg aaaatccgcg
    13141 agatgcgatt cccctttcag cttcgctgcc gcgctgtagg ttaatgtgtt tacatgatag
    13201 cggctttcga gtttaccgga cggtccgcca ataattggta cgcagattaa ctgccgcgat
    13261 tgccccgcct gcggcaagtt ttcaaccagt gcgctcaccg cgcgacccca ggaaaaaccg
    13321 acaatatcgc caggctccag caagcgatcc agcagttgcg cgccgtgtaa ccccatcatc
    13381 gccagttgag tctcttcatc ctcatcattt cccgacacca ccacaacgtc tttcaggcca
    13441 aacttttgct tcagttgctg ctccagccag agattttcgt tgtagtcata gttgatggcg
    13501 atggtgacaa ttccctgatc gcggcctcgt ttaagcaagc ggctgatggt ggtgcggtaa
    13561 atacgcagtt cgcgcgcgat ttgcgcctgc gtcatatcct gttcgtaata gagttgggca
    13621 atcttcacaa tcaaacggat atcgtcactg ttttccattg ccgcctcctg gttctgcaca
    13681 tttgtgcaaa tacgctttct caccgcttga tctgatataa ccccatcatg agggcctttc
    13741 aaagcgattt ttcactcttt gcatggggag aaatagcgct ttctcttgca catttgtttc
    13801 tctgcgtagt agtcatcagc acaggtgtgc agcgattcgt aatatgttga ttatccttat
    13861 tttgccaggc aaatttgcat cgtacttccg gagacacttt tgtgataaaa ggcgtggcag
    13921 atcacattta atttcgacgt tgccgcataa ccctgttttt ccccgctaag aggtacggta
    13981 tattccgcgt tttacaggag aatttatgct gcccgactca tcagtccgtt taaataaata
    14041 catcagcgaa agcggaattt gctcacgccg cgaagcggat cgctatatcg agcaaggcaa
    14101 tgtgttcctt aatggcaagc gagccaccat tggcgatcag gtgaaacccg gcgacattgt
    14161 gaaagtaaac ggtcagttga ttgagcctcg ggaagccgaa gatttggtac ttatcgccct
    14221 gaacaagccc gtcggtattg tcagcaccac cgaagatggc gagcgcgata acattgtcga
    14281 tttcgttaac cacagcaaac gcgtgttccc gattggccgc ctggataaag actcccaggg
    14341 gctgattttc ctcaccaatc acggcgatct ggtgaataag atcctccgtg cgggtaacga
    14401 tcatgagaaa gagtatctgg tgacggtcga taaaccgatt accgatgaat ttattcgtgg
    14461 tatgggggcg ggggtgccga tcctcgggac ggtgacgaaa aagtgcaaag tcaaaaaaga
    14521 agcgccgttt gtcttccgca ttaccctggt gcaggggctg aaccgtcaga tccggcgcat
    14581 gtgcgagcat ttcggctatg aagtgaaaaa gctggaacgt acgcgcatta tgaacgtcag
    14641 tttaagcggc attccgctgg gtgagtggcg cgatttaact gacgatgagt taatcgacct
    14701 gtttaaactc attgaaaatt cctcttccga ggtaaaacct aaagcgaagg ctaaaccgaa
    14761 aacagcgggc atcaaacgtc cagtcgttaa gatggaaaaa acggcggaaa aaggcggtcg
    14821 cccggcgtcc aacggtaagc gttttacctc gccggggcgt aaaaagaagg gacgctaatc
    14881 agcgcctgcc gcgcgtcgac gtatttgctg accagctaaa tgacgcttca ggatccggtt
    14941 tataagcctg ctttttcttc aactggcggg cttttttcgc ttcagcctca cgctcgacca
    15001 ttaatttatc gatgtactct ttcttaatgc tgttggtttc ggagttggtc agtacgcgac
    15061 catgcgcaat ccgcgcccga tccagcagtg ttttaagttc acgctgctcg cgttcagtca
    15121 tctctttttg ggtaatacgg gggagtgcca tgatggtgcc ctcagtgagc cagagagttc
    15181 agtgtacggg aattcgtttg cgagctgaac tgataatatt cctttgttct cttcttaaat
    15241 atttatcttt cttgataatt gacgataaat acagatacaa atttagcgaa aagttt
//
LOCUS       DAZGOB010000120        15271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGOB010000120 DAZGOB010000000
VERSION     DAZGOB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.120"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            271..606
                     /locus_tag="ACHLIF_003905"
     CDS             271..606
                     /locus_tag="ACHLIF_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO9607826.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            766..1209
                     /gene="yjdN"
                     /locus_tag="ACHLIF_003906"
     CDS             766..1209
                     /gene="yjdN"
                     /locus_tag="ACHLIF_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO9607827.1"
                     /translation="MSLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1342..2130
                     /gene="phnC"
                     /locus_tag="ACHLIF_003907"
     CDS             1342..2130
                     /gene="phnC"
                     /locus_tag="ACHLIF_003907"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO9607828.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2155..3171
                     /gene="phnD"
                     /locus_tag="ACHLIF_003908"
     CDS             2155..3171
                     /gene="phnD"
                     /locus_tag="ACHLIF_003908"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9607829.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3277..4056
                     /gene="phnE"
                     /locus_tag="ACHLIF_003909"
     CDS             3277..4056
                     /gene="phnE"
                     /locus_tag="ACHLIF_003909"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO9607830.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRVINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4077..4802
                     /gene="phnF"
                     /locus_tag="ACHLIF_003910"
     CDS             4077..4802
                     /gene="phnF"
                     /locus_tag="ACHLIF_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO9607831.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4803..5255
                     /gene="phnG"
                     /locus_tag="ACHLIF_003911"
     CDS             4803..5255
                     /gene="phnG"
                     /locus_tag="ACHLIF_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO9607832.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5252..5836
                     /gene="phnH"
                     /locus_tag="ACHLIF_003912"
     CDS             5252..5836
                     /gene="phnH"
                     /locus_tag="ACHLIF_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO9607833.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5836..6900
                     /gene="phnI"
                     /locus_tag="ACHLIF_003913"
     CDS             5836..6900
                     /gene="phnI"
                     /locus_tag="ACHLIF_003913"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO9607834.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6893..7738
                     /gene="phnJ"
                     /locus_tag="ACHLIF_003914"
     CDS             6893..7738
                     /gene="phnJ"
                     /locus_tag="ACHLIF_003914"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO9607835.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7735..8493
                     /gene="phnK"
                     /locus_tag="ACHLIF_003915"
     CDS             7735..8493
                     /gene="phnK"
                     /locus_tag="ACHLIF_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO9607836.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMGEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8604..9284
                     /gene="phnL"
                     /locus_tag="ACHLIF_003916"
     CDS             8604..9284
                     /gene="phnL"
                     /locus_tag="ACHLIF_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO9607837.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9281..10417
                     /gene="phnM"
                     /locus_tag="ACHLIF_003917"
     CDS             9281..10417
                     /gene="phnM"
                     /locus_tag="ACHLIF_003917"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO9607838.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10417..10974
                     /gene="phnN"
                     /locus_tag="ACHLIF_003918"
     CDS             10417..10974
                     /gene="phnN"
                     /locus_tag="ACHLIF_003918"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO9607839.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10961..11395
                     /gene="phnO"
                     /locus_tag="ACHLIF_003919"
     CDS             10961..11395
                     /gene="phnO"
                     /locus_tag="ACHLIF_003919"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO9607840.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11397..12155
                     /gene="phnP"
                     /locus_tag="ACHLIF_003920"
     CDS             11397..12155
                     /gene="phnP"
                     /locus_tag="ACHLIF_003920"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO9607841.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12302..12631)
                     /gene="yjdP"
                     /locus_tag="ACHLIF_003921"
     CDS             complement(12302..12631)
                     /gene="yjdP"
                     /locus_tag="ACHLIF_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO9607842.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12700..13017)
                     /locus_tag="ACHLIF_003922"
     CDS             complement(12700..13017)
                     /locus_tag="ACHLIF_003922"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO9607843.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13270..15255
                     /gene="yjcS"
                     /locus_tag="ACHLIF_003923"
     CDS             13270..15255
                     /gene="yjcS"
                     /locus_tag="ACHLIF_003923"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO9607844.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         3476 a         4509 c         4193 g         3093 t
ORIGIN      
        1 acgtcttccg atacgtttaa aacatcagaa acataaaaat atcatgtgaa ttaaaaaaaa
       61 agaacaagta gagcattaac attatcttaa ataataaatg gaggcaaaaa gattattttc
      121 tgtttgcgtt tcctttcaaa tgaaaacgat cgtcgtctaa aatcagcagt acccccgaca
      181 aactcaggga ttttgtgtat aattgcggcc tttttcggca atctgccgtt ttttggcgct
      241 tttgccctgc tgacttttga ggaaatccac atgtcattac cacactgccc aaaatgcaac
      301 tccgaataca cttacgaaga taacggcatg tacatctgcc cggaatgtgc ctacgaatgg
      361 aacgacgcag aacctgcaca ggaaagcgac gagctgatcg ttaaagatgc taacggcaat
      421 ctgctggctg acggcgacag cgttaccatc attaaagatc tgaaggtgaa aggtagctct
      481 tcgatgctga aaattggcac caaagtgaaa aacatccgcc tggttgaagg cgaccataac
      541 atcgattgca aaatcgacgg ttttggtccg atgaaactga aatctgagtt tgtgaaaaag
      601 aactgattgt attgtgatcg gtaagccgga taaggcgctc gcgccgcatc cggcaacgat
      661 gccgactgcc tgatgcgacg ctgaccgcgt cttatcaggc ttccctctca tctgtataaa
      721 tttcgaacta cacttaactg gcttctctta actgaggtca ccatcatgtc gttaagtccc
      781 tacctctctt ttgccggtaa ctgtgccgac gcaattgctt attatcaacg tacgttgggc
      841 gctgaactgc tctataaaat cagcttcggc gaaatgccaa aatcagcgca ggacagcgcc
      901 gagaactgcc cttccggaat gcaatttccc gataccgcca tcgcccacgc caacgtgcgc
      961 attgccggaa gcgacatcat gatgagcgat gctataccgt caggaaaagc cagctactcc
     1021 ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag gaaaacgctg gtttgacaat
     1081 cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa ctttctgggc gcatggcttt
     1141 ggcaaagtca ccgataaatt tggcgtaccg tggatgatta atgtcgtcaa acaacaacca
     1201 acgcaataac ccgccgggag gcccgccctc ccgcactgtc atcgaattcc cgttaactct
     1261 tcatctgtta gtcactttta attaaccaaa tcgtcacaat aatccgccac gatggagcca
     1321 cttttttagg gaggctgcat catgcaaacg attatccgtg tcgagaagct cgccaaaacc
     1381 ttcaatcagc atcaggcgct gcatgcggtt gatctgaaca ttcatcacgg tgaaatggtg
     1441 gctctgcttg ggccgtcggg ttccggcaaa tccacccttt tacgtcactt aagcggtttg
     1501 attaccggcg ataaatccgc cggcagccat atcgagctgc tgggccgcac agtccagcgc
     1561 gaaggccgtc tggcgcgcga tatccgcaaa agccgcgcca acaccggcta catcttccaa
     1621 caattcaacc tggtgaaccg cctgagcgta ctggagaacg tgctgattgg cgcgctcggc
     1681 agcacgccgt tctggcgcac ctgttttagc tggttcaccg gcgagcagaa acagcgcgcg
     1741 ttacaggcgc tgacccgcgt tggcatggtg cattttgccc atcagcgcgt ttccaccctc
     1801 tccggcggac agcagcagcg tgtggcgatt gcccgcgcgc tgatgcagca ggcgaaggtg
     1861 attctggccg atgaacccat cgcctcgctg gacccggaat ccgcccgcat cgtgatggac
     1921 accctgcgcg acatcaatca gaacgacggc atcaccgtgg tcgtcacgct gcatcaggtg
     1981 gattacgccc tgcgctactg cgaacgcatc gtcgccctgc gccaggggca cgttttctac
     2041 gacggcagca gccaacagtt tgataacgaa cgttttgacc atctctaccg cagcattaat
     2101 cgcatcgaag agaacgcgaa agctgcctga catccccatc attgaggaaa acgaatgaac
     2161 gctaagataa ttgcctcgct ggccttcacc agcatgttca gcctcagcac cctgttaagc
     2221 ccggcacacg ccgaagagca ggaaaaggcg ctgaatttcg gcattatttc aacggaatca
     2281 cagcaaaacc tgaaaccgca atggacgcca ttcttacagg atatggagaa gaagctgggc
     2341 gtgaaggtga acgccttctt tgccccagac tacgcaggca ttatccaggg aatgcgcttc
     2401 aataaagtgg atatcgcctg gtacggcaac ctgtcggcaa tggaagcggt ggatcgcgcc
     2461 aacggccagg tcttcgccca gacggtcgcg gcggatggat cgccaggtta ctggagcgtg
     2521 ttgatcgtca acaaagatag tccgatcaac aacctgaacg atctgctggc gaagcggaaa
     2581 gatctcacct tcggcaatgg cgatcctaac tccacctctg gcttcctcgt ccccggttac
     2641 tacgtctttg ccaaaaacaa tatctccgcc agcgacttta agcgcaccgt caacgctggg
     2701 catgaaacca acgcgctggc cgtcgccaac aagcaggtgg atgttgccac caacaacacc
     2761 gaaaacctcg acaagctgaa aacctccgcg ccagagaagc tgaaagaact gaaggtgatc
     2821 tggaagtcgc cgctgatccc aggcgatccg atcgtctggc gtaaaaacct ttccgaaacc
     2881 accaaagaca agatctacga cttctttatg aactacggca aaacgccgga agaaaaagcg
     2941 gtgctggaac gcctgggctg ggcgccgttc cgcgcttcca gcgacctgca actggtgccg
     3001 attcgccagc tcgcgctgtt taaagagatg cagggcgtga aaagcaataa aggactgaat
     3061 gagcaggaca agctggcaaa aaccaccgag attcaggcgc agctggatga cctggaccgc
     3121 ctgaacaacg cgttaagcgc gatgagttcg gtgagtaaag cggtgcagta aatcgtaggt
     3181 cggataagac gccccggcgt cgcatccgac aatgtgcagg cgttgatgcc ggatgcggtg
     3241 caagcacctt atccggccta cagaccggag ccaaacatgc aaaccatcac catcgcccca
     3301 cccaagcgca gctggttctc gcttctgagc tgggccgttg ttctcgccgt gttggtcgtc
     3361 tcgtggcagg gcgcggaaat ggccccgctc acgctgatta aagacggcgg caacatggcg
     3421 acgttcgccg ccgacttctt cccgcccgat ttcagccagt ggcaggatta cctcaccgaa
     3481 atggccgtca cgctgcaaat cgccgtctgg ggcacggcgc tggcggtggt tctctccatc
     3541 cccttcggcc tgatgagcgc cgaaaacctg gtgccgtggt gggtttacca gcccgttcgc
     3601 cgcctgatgg acgcctgccg cgtcattaac gaaatggtct tcgccatgct gttcgtggtc
     3661 gccgtcggcc tcggcccgtt cgctggcgtg ctggcgctgt ttatccacac caccggcgtg
     3721 ctctccaaac tgctttccga agcggtggaa gcgattgaac ccggcccggt ggaaggcatt
     3781 cgcgccaccg gcgccaacaa gctcgaagag atcctctacg gcgtgctgcc gcaggtgatg
     3841 ccgctgctga tctcctactc cctctatcgc ttcgaatcca acgtccgctc ggcgaccgtc
     3901 gtcggcatgg tcggtgcagg cgggatcggc gtcaccctgt gggaagcgat tcgcggtttc
     3961 cagttccaac aaacctgcgc cctgatggtg cttatcatcg tcacggtcag cctgctggat
     4021 ttcctctctc aacggttgcg taagcacttt atctgataag cgaggcattg atatctatgc
     4081 acttgtctac acatccgacc agctacccaa cacgctatca ggagatagcc gcaaaacttg
     4141 agcaggagct tcgtcaacac taccgctgcg gcgactatct tcccgccgag cagcaactgg
     4201 cggcgcgctt tgaggtgaac cgccacaccc tgcgccgcgc catcgaccaa ctggtggaaa
     4261 aaggctgggt acagcgccgt cagggcgtcg gcgtgctggt gctgatgcgc ccgttcgatt
     4321 acccgctcaa cgcccaggcg cgttttagcc agaacctgct ggaccagggt agccatccca
     4381 ccagcgaaaa actgctgtcg gtattgcgcc ccgcgtccgg tcacgtcgct gacgcgctgg
     4441 ggattaccga gggcgagaac gttatccatt tgcgcaccct gcgtcgcgtc aacggcgtcg
     4501 cgctctgttt aatcgaccac tactttgcgg acctcaccct ttggccgacg ctgcaacgct
     4561 tcgacagcgg ttcgctgcac gattttctgc gcgagcaaac cggaattgcg ctgcgccgca
     4621 gccagacgcg gatcagcgcc cgtcgcgccc aggccaaaga gtgccagcgt cttgaaattc
     4681 cgaatatgtc gccgctgctg tgcgtgcgca cccttaacca ccgtgacggt gaaagcagcc
     4741 cggcggagta ctccgtcagc ctgacgcgcg ccgacatgat tgaattcact atggagcact
     4801 gaatgcacgc agataccgcg acccgccagc actggatgtc cgtactggcg cacagccaac
     4861 cggctgaact ggcagcccgc ctgaaagcac tgaacatcac cgccgactat gaggttatcc
     4921 gcgccgccga aaccggcctg gtgcagattc aggcgcggat gggcggcacc ggcgaacgct
     4981 tttttgccgg cgacgccacg ctcacccgcg ccgccgtacg cctgactgac ggcacgctcg
     5041 gctacagctg ggtgctgggg cgcgacaaac agcacgccga acgctgcgcg ctgattgacg
     5101 cgctgatgca gcaatctcgc tattttcaaa acttatcaga aacccttatt gccccgctgg
     5161 acgctgaccg tatggcacgc attgccgcac gccaggccga agtgaacgcc agccgggtcg
     5221 acttctttac gatggttcgc ggagacaacg catgaccctg gaaaccgctt ttatgcttcc
     5281 cgtgcaggat gcccagcaca gttttcgtcg cctgttaaag gccatgagcg agccgggcgt
     5341 gattgtcgcc ctgcatcagc tcaagcgcgg ctggcagccg ctgaatatcg ccaccaccag
     5401 cgtgctgctg acgctggccg ataacgacac gccggtgtgg cttgccgcgc cattgagtaa
     5461 cgatatcgtc agccagagcc tgcgttttca taccaacgcg ccgctggtca gccagccgga
     5521 acaggcgacc ttcgcggtga cggatgaggc gatttccagc gaacagctta acgccctttc
     5581 caccggcacc gccgttgcgc cggaagcggg tgcaacgctg attttacagg tcgccagcct
     5641 gagcggcggg cgcatgttac gtctcaccgg cgcgggcatt gccgaagaac gaatgatcgc
     5701 tccgcagctg ccggagtgca ttctgcacga actcaccgag cgcccgcacc cgttcccgct
     5761 cggcatcgac ctgatcctga cctgcggcga gcgcctgctg gctattccgc gaaccactca
     5821 tgtggaggtg tgctgatgta cgttgccgtg aaagggggcg agaaggcgat cgacgccgct
     5881 cacgccctgc aagagagccg acgccggggc gataccgatt tgcctgaact gagcgtcgcc
     5941 cagattgaac agcagcttaa tcttgcggtg gatcgcgtga tgaccgaagg cggcattgcc
     6001 gaccgcgaac tggcggcgct ggcgctgaaa caggccagcg gcgataacgt cgaagcaatt
     6061 ttcctgctgc gcgcctaccg caccacgctg gcgaagctcg cggtaagcga gccgctcgac
     6121 accacagaga tgcgtctcga acggcgtatt tcggcggttt ataaagacat tcccggcggc
     6181 cagctgcttg gcccaaccta cgactacacc catcgcctgc tcgatttcac cctgctggca
     6241 aacggtgaag cgccgacgct gaccaccgcc gacagcgaac aacagccgtc gccgcacgtt
     6301 ttcagcctgc tggcgcgtca ggggctggtg aagtttgaag aagatagcgg cgcacagccg
     6361 gatgacatca cccgcacgcc gccggtttac ccctgctcac gctcctcccg cctgcaacaa
     6421 ttaatgcgcg gggacgaagg ctatttgctg gcgctggcct actccaccca gcgtggttac
     6481 gggcgcaatc acccgttcgc gggcgagatc cgcagtggtt acatcgacgt gtcgattgtg
     6541 ccggaagagc tgggatttgc ggtaaacgtc ggcgaactac tgatgaccga gtgtgaaatg
     6601 gtcaacggtt ttatcgaccc gccggatgag ccgccgcact tcacgcgcgg ctacgggctg
     6661 gtattcggca tgagcgagcg caaagcgatg gcaatggcgc tggtcgatcg tgcgttgcag
     6721 gctccggaat acggcgagca cgcgacaggc ccggcgcagg atgaagagtt tgtgctggca
     6781 catgccgaca acgtcgaagc cgcaggcttt gtctcgcacc tcaaactccc ccactacgtc
     6841 gatttccagg ccgaactgga gctactcaaa cgtctgcaac aggagaagaa ccatggctaa
     6901 tctgagcggc tacaactttg cctacctcga cgagcagacc aaacgcatga tccgccgcgc
     6961 tatcttaaaa gcggtggcga tccccggtta tcaggtgccg tttggcgggc gcgagatgcc
     7021 gatgccatac ggctggggaa ccggcggcat acagctcacc gccagcgtga ttggcgaaag
     7081 cgacgtgctg aaggtgattg accagggtgc ggatgacacc accaacgccg tgtcgattcg
     7141 caacttcttt aagcgcgtga ccggggtaaa caccactgaa cgtacggacg atgcgacgct
     7201 tatccagacg cgtcaccgca tccccgaaac gccgctgacc gaagatcaga tcattatctt
     7261 ccaggtgcca atcccggaac cgctgcgctt tatcgagccg cgcgaaacgg aaacccgcac
     7321 catgcacgcg ctggaagagt acggcgtgat gcaggtgaaa ctgtatgaag atatcgcccg
     7381 cttcggtcat atcgccacta cctatgccta tccggtgaag gtgaacgggc gctacgtaat
     7441 ggacccgtcg ccgatcccga aattcgataa cccaaaaatg gacatgatgc ccgccctgca
     7501 actgttcggc gcggggcgcg agaagcgcat ctatgcggtg ccgccgttta cccgcgtgga
     7561 aagtctcgat ttcgacgatc acccgttcac cgttcagcag tgggatgagc catgcgccat
     7621 ctgcggatcg acccacagct atcttgatga agtggtgctg gatgacgccg gaaaccgcat
     7681 gtttgtctgc tccgataccg attattgccg ccaacagagc gaggcaaaaa accaatgaat
     7741 caaccgttac tttcggtcaa taacctgacc cacctttacg cgccgggcaa aggctttagc
     7801 gatgtctctt ttgatttatg gccgggggaa gtgctgggca ttgtcgggga gtccggctcc
     7861 gggaagacca cgctgctgaa atcaatctcc gcgcgcctga cgccgcagca gggggagatt
     7921 cactatgaga gccgttcgct gtacgcaatg ggcgaggccg accgccgtcg cctgctgcgc
     7981 accgaatggg gcgtagtgca tcagcatcca ctcgacggcc tgcgccgtca ggtctcggca
     8041 ggcggcaata tcggcgagcg gctgatggcg accggggcac gtcattacgg cgatattcgt
     8101 gccaccgcgc agaagtggct ggaagaggtg gaaattcccg ccaaccggat cgacgacctg
     8161 ccgaccacct tttccggcgg catgcagcaa cgtttgcaga tcgcccgcaa cctggtgacg
     8221 catccgaagc tggtgtttat ggacgagccg accggcggac tggatgtgtc ggtacaggca
     8281 cgcctgctcg acctgctgcg cggcctggtg gtggagctga acctcgcggt agtgattgtc
     8341 acccacgatt taggcgtcgc ccgcctgctg gcggaccgtt tgctggtgat gaagcagggg
     8401 caagtggtgg agagtgggtt aaccgaccgc gtgctcgacg acccgcacca tccgtataca
     8461 caactgctgg tgtcatcggt tttgcagaat tgagccggtg ccggatgcgg cgtaaacgcc
     8521 ttatccggcc tacaaatgcg ctccccgtag gtcggataag acgcgtcagc gtcgcatccg
     8581 acacccgaac cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa accttcatcc
     8641 tgcaccagca aaacggcgtg cgcctgcccg tcttacaacg tgcctcgctc accgtcaacg
     8701 cgggcgaatg cgtggtgctc cacggccatt ccggcagcgg caaatcaacc ttgctgcgct
     8761 cgctgtacgc caactatctg cccgacgaag ggcaaatcca gatcaaacac ggtgacgaat
     8821 gggtcgatct ggtcaccgcg ccagcgcgca aagtggtgga gatccgcaaa accaccgtcg
     8881 gctgggtgag ccagtttctg cgcgtcatcc cgcgtatttc agcactggaa gtggtgatgc
     8941 agccgctgct cgataccggc gttccgcgtg aagcctgcgc cgctaaagcc gcgcgtctgc
     9001 tcacccgcct gaacgtgccg gaacgcctgt ggcacctggc gccatcaacc ttttccggtg
     9061 gcgaacagca gcgcgtcaac atcgcccgcg gctttatcgt cgactacccc attctgctgc
     9121 tcgacgaacc taccgcctcg ctggacgcca aaaacagcgc cgccgtagtc gagctgattc
     9181 gcgaagccaa aacccgtggc gcggccatcg tcggcatctt ccatgacgaa gccgtacgta
     9241 atgacgtcgc cgaccgcttg cacccaatgg gagcctctgc atgattatca ataacgtcaa
     9301 actggtgctg gaaaacgaag tggtacacgg ttcgctggag gtgcaggatg gcgaaatccg
     9361 cgcctttgcc gaaagccaga gccgcctgcc agaagcgatg gacggcgaag gtggctggct
     9421 actacccggc ctgattgagc tgcataccga caacctcgat aaattcttca ccccgcgccc
     9481 gaaagtcgac tggcccgccc attcggcgat gagcagccac gacgcgctga tggtggcaag
     9541 cggcatcacc accgtactgg acgccgtggc gattggcgac gtgcgcgacg gcggcgatcg
     9601 gctggagaat ctggagaaga tgatcaacgc catcgaagag acgcaaaaac gcggcgtcaa
     9661 ccgcgccgag caccgcctgc acctgcgctg cgaactgccg catcacacca cactgccgct
     9721 gtttgaaaaa ctggttcagc gcgaaccggt gacgctggtg tcgctgatgg accactcacc
     9781 gggccagcgc cagttcgcta accgcgagaa gtatcgcgaa tattatcagg gcaaatactc
     9841 cctcaccgat gcgcagatgc agcagtacga agaagagcaa ttggcgctcg ccgcacgctg
     9901 gtcgcagccg aatcgcgaat ccatcgccgc cctgtgccac gcgcgacaaa ttgcgctcgc
     9961 cagccacgat gacgccacgc acgcccacgt tgccgaatcc caccaacttg gcagcgtgat
    10021 cgccgaattt cccaccacgt tcgaagcggc ggaagcctcg cgtaagcatg gcatgaacgt
    10081 gctgatgggt gcgccaaata tcgtgcgtgg cggctcgcac tccggcaatg tggcggccag
    10141 tgaactggcg cagcttggtc tgctggatat cctctcttcc gactactacc ccgccagcct
    10201 gctggatgcg gcgttccgcg tcgccgatga cgagagcaac cgctttacgc taccgcaggc
    10261 ggtgaggctg gtgaccaaaa atccggcgca ggcgctgaat cttcaggatc gcggggtgat
    10321 tggcgagggt aaacgcgctg acctggtgct ggcgcatcgc cagggcaatc acattcatat
    10381 cgaccacgtc tggcgtcagg gtaaaagggt gttctgatga tgggaaaact gatttggtta
    10441 atggggccgt ccggctccgg gaaagacagc ctgctggcgg aactccgcct gcgggaacaa
    10501 acgcagttac tggtggcgca tcgctacatc acgcgcgacg ccagcgccgg aagtgaaaac
    10561 catatcgccc tgagtgagcg ggagtttttt acccgcgcgg ggcaaaacct gctggcctta
    10621 agctggcacg ctaacggtct gtattatggc gtcggcatcg agattgacct ctggctgcac
    10681 gctggattcg acgtagtcgt caacggttca cgcgcgcatt tgccgcaggc acgggcgcgc
    10741 tatcaatcgg cgctgctgcc cgtctgttta caggtttcgc cggagatcct gcgccagcgc
    10801 ctggaaaacc gtggccgtga aaacgccagt gaaattaacg cccggctggc gcgcgccgcc
    10861 cgctatactc cacaggattg ccatacgctc aacaatgacg gcagcctgcg ccagtcggtc
    10921 gacacactgc tgacgctgat tcatcagaag gagaaacacc atgcctgctt gtgagcttcg
    10981 cccggccacg cagtacgata ccgacgcggt ttacgcgctg atttgtgagc taaaacaggc
    11041 ggagtttgac caccacgcgt ttcgcgtggg ctttaacgcc aacctgcgcg acccgaacat
    11101 gcgctaccat ctggcgctgc tggatggcga agttgtcggc atgatcggcc tgcatttgca
    11161 gtttcatctg catcatgtca actggatcgg cgaaattcag gaactggtgg tgatgccgca
    11221 ggcgcgcggc ctgaacgtcg gtagcaagtt gctggcgtgg gcagaagaag aagcccgcca
    11281 ggccggggcc gaaatgaccg agctttcgac caacgtgaag cgccacgacg cgcaccgttt
    11341 ctatctgcga gaaggctacg agcagagcca cttccgcttc accaaggcgc tgtaacatga
    11401 gcctgaccct cacgctcacc ggcactggcg gcgcacaggg cgttccggca tggggctgcg
    11461 agtgcgcggc ctgcgccaga gcgcggcgct cgccgcagta tcgccgccag ccatgcagcg
    11521 gcgtagtgaa gtttaacgac gcgatcaccc tgatcgacgc cgggcggcac gatctcaccg
    11581 atcgctggtc gcctggatcg ttccagcagt ttttgctgac gcactaccat atggatcacg
    11641 tccaggggct atttccgcta cgctggggcg tgggcgatgt gatcccggtc tacggcccgc
    11701 cggatgaaca gggctgcgac gatctgttta aacatccggg cctgcttgat ttcagccaca
    11761 cagtggaacc gtttgtggtg tttgatttgc aggggttgca ggtcacgccg ctgccgctta
    11821 accactcaaa actgaccttc ggttatctgc tggaaacggc acatagccgg gtggcgtggc
    11881 tgtctgacac cgctggcctg ccggaaaaaa cgctgaaatt tttactcaat aatcatccgc
    11941 aggtgatggt gattgattgc agtcacccgc cgcgcgcgga tgcaccgcgt aatcactgtg
    12001 atttaaatac cgtgcttgcg ctgaatcagg ttatccgctc gccacaggtg attctgaccc
    12061 atatcagcca ccagtttgat gcgtggctga tggaaaacgc actaccgtca gggtttgagg
    12121 tggggtttga tgggatggag attggggtgg cgtgatgaga gggaatgtgc gcgctggccc
    12181 cctcacccta accctctccc cagaggggcg aggggaccga ttgtgctcaa tattgaatat
    12241 tgcgctcgtt ttctccctct ccccattggg gtgaggggcg atgcctgctc catacccaac
    12301 ctcatcgccc atactcatct tccattctcc gctcttcatc ctccagttgc cgacgctcct
    12361 gatcaagctg gcgctggcga tcgtccagct gcctgcggcg atcttcaaac tggcggcggc
    12421 ggtcgtcata ttgtctgcgc cgatcgtcgc tcacttcacg ctgccagccg tcgtcgcgcg
    12481 aatcttcata gtctcgccca cggtcagggt tataagcgtc attaatcgcc tgctgaatat
    12541 taccaatagt atcgtcgata atatcagcct gggccggaac ggtggacagc gtgagcaggg
    12601 tgaataaaag aaatagcgga aagcgtttca ttagccaacc tcaaaaagaa actctatcca
    12661 cattagtcat tactcatcca tgcaagtagt ggatgaatct caatttctcc gctgctctat
    12721 tgccgtaatc gcctccacgc gttgttgatg acgaccgcct tcgtactgtg cgcccagcca
    12781 cgcatccaca atcatttttg ccagttcgag gccaaccact cgtgaaccaa aagccagtgc
    12841 aacttcactg gcataatgcg gataatcagt gcgctctgac gacctggtgt ctttatcaat
    12901 cacctcactc attgaccatg aataaaaggc tggcacagca acacagaatc ctgcggtgcc
    12961 agcgacatga ccgcccattg ctccggtacg ttttttaaaa agaaacaccg gttgcattga
    13021 taaattgtgc tcagtatttg ccgaagtaca atatatataa taaatatata aaacacctca
    13081 tattatttac agagataata aattcgcgta attttccccc ttcagcacaa atgcctaata
    13141 aaatggatcg ttccgctcac acttccactt attaaagtcg caaattccca attatgataa
    13201 cgccagcttt aaatgacgta ttctgcatgg attacatatt tacgacacaa atgataaatg
    13261 gagatttata tgaataactc tcggttattc cgtttgagca ggattgttat tgcgttaact
    13321 gctgccagcg gcatgatggt aaataccgct aacgcgaaag aggaagcgaa agccgccact
    13381 caatataccc aacaggttaa tcagaattac gccaaatcat taccgtttag cgatcgtcag
    13441 gattttgacg acgctcagcg tggatttatc gctccgctgc tggatgaagg tattctgcgc
    13501 gatgctaatg gcaaaccata ttatcgcggg gaagattata aatttgatat caatgcgccc
    13561 gcgcccgaaa ccgttaaccc tagcctgtgg cgtcagtcgc agttaaacgg tatttccggc
    13621 ctgtttaaag tcaccgacag aatgtaccag gttcgcggtc aggatatctc caacatcacc
    13681 tttatcgaag gtgataccgg gattattgtc atcgacccgc tggtgacgcc accgagcgca
    13741 aaagccgccc ttgaccttta cttccaaaat cgcccgcaaa aaccgattgt tgcggttatt
    13801 tatacccaca gccataccga ccactatggc ggcgtgaaag gcattatctc tgaagctgat
    13861 gtgaaatccg gtaaagtgca ggttatcgcc cctgctggct ttatggacga agccatcagc
    13921 gaaaacgtac tggcgggtaa tattatgagc cgccgtgcac tttactccta cggcctgctg
    13981 ctggcgcata accctcaggg taacatcggc aatggtcttg gcgtaacgct ggcatcgggc
    14041 tacccgagca tcatcgcacc gaacaaaacc atcaccaaaa ccggtgagaa gatgattatc
    14101 gacggcctgg agtttgactt cctgatgacc ccaggtagcg aagcaccagc cgaaatgcac
    14161 ttctatattc cggccctgaa agcgctgtgt accgccgaga acgccacgca taccctgcac
    14221 aacttctaca ctctacgcgg cgcgaaaacc cgcgacacca gcaagtggac cgagtatctg
    14281 aacgaaacgc tggatatgtg gggtaacgac gcggaagtcc tgtttatgcc gcacacctgg
    14341 ccggtctggg gcaataagca tatcaatgat tatattggta aatatcgcga tactatcaag
    14401 tacattcacg accagaccct gcacctggcg aaccagggct acaccatgaa tgaaatcggc
    14461 gacatgatta aactgccgcc tgcacttgcc aataactggg ccagccgtgg ctattacggt
    14521 tctgtcagcc acaacgcccg cgcggtgtat aacttctatc ttggctatta cgacggtaac
    14581 ccggctaacc tgcatccgta tggtcaggtg gagatgggta aacgttacgt gcaggcgctg
    14641 ggcggttctg cccgtgtcat caacctggcg caagaagcga acaagcaagg tgattaccgc
    14701 tggtcggcag aactgctgaa acaggtgatt gccgccaacc cgggtgacca ggtcgcgaag
    14761 aatctgcaag cgaataactt tgaacagctg ggctatcagg ccgagtccgc cacctggcgc
    14821 ggtttctacc tgaccggcgc gaaagagctg cgcgaagggg tgcataagtt cagccacggc
    14881 accaccggtt ccccggacac cattcgcggg atgtcggtcg aaatgctgtt cgactttatg
    14941 tccgttcgcc tcgatagcgc gaaagccgcg ggtaaaaata tcagcctgaa cttcaatatg
    15001 agcaatggcg ataacctcaa cctgacgctg aacgatagcg tgcttaacta ccgtaaaaca
    15061 ctgcaatccc aagctgacgc ctctttctac atcagccgtg aagatctgca cgccgtgctg
    15121 accgggcaag ccaaaatggc ggatctggta aaagcgaaga aagccaaaat tattggcaat
    15181 ggcgcgaaac tggaagaaat tatcgcctgt ctggataatt tcgatttgtg ggtgaatatc
    15241 gtaaccccaa attaatgtca ctcgcttttg g
//
LOCUS       DAZGOB010000121        15267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGOB010000121 DAZGOB010000000
VERSION     DAZGOB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.121"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1412)
                     /locus_tag="ACHLIF_003924"
     CDS             complement(<1..1412)
                     /locus_tag="ACHLIF_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9607845.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLTD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSSSGAEATGVTTLLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEA"
     gene            1656..1979
                     /locus_tag="ACHLIF_003925"
     CDS             1656..1979
                     /locus_tag="ACHLIF_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO9607846.1"
                     /translation="MNWIVIDTVIQPACDISFSAIWCNIKLILWYQSDIFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVTPFNKNVWNLIKSSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
     gene            complement(2277..2546)
                     /locus_tag="ACHLIF_003926"
     CDS             complement(2277..2546)
                     /locus_tag="ACHLIF_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO9607847.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(2558..3517)
                     /locus_tag="ACHLIF_003927"
     CDS             complement(2558..3517)
                     /locus_tag="ACHLIF_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO9607848.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            complement(3608..3684)
                     /locus_tag="ACHLIF_003928"
     tRNA            complement(3608..3684)
                     /locus_tag="ACHLIF_003928"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3648..3650),aa:Arg,seq:tct)
     gene            complement(3698..3774)
                     /locus_tag="ACHLIF_003929"
     tRNA            complement(3698..3774)
                     /locus_tag="ACHLIF_003929"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3738..3740),aa:Arg,seq:tcg)
     gene            complement(3784..3859)
                     /locus_tag="ACHLIF_003930"
     tRNA            complement(3784..3859)
                     /locus_tag="ACHLIF_003930"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3824..3826),aa:Ile,seq:cat)
     gene            complement(3900..4958)
                     /locus_tag="ACHLIF_003931"
     CDS             complement(3900..4958)
                     /locus_tag="ACHLIF_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO9607849.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            complement(5110..5307)
                     /locus_tag="ACHLIF_003932"
     CDS             complement(5110..5307)
                     /locus_tag="ACHLIF_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9607850.1"
                     /translation="MLKQQDMTETARVVFNELSATAPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(5523..5903)
                     /locus_tag="ACHLIF_003933"
     CDS             complement(5523..5903)
                     /locus_tag="ACHLIF_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9607851.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTTKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(5922..6911)
                     /locus_tag="ACHLIF_003934"
     CDS             complement(5922..6911)
                     /locus_tag="ACHLIF_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9607852.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(6963..7220)
                     /locus_tag="ACHLIF_003935"
     CDS             complement(6963..7220)
                     /locus_tag="ACHLIF_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607853.1"
                     /translation="MNNRTTTVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            complement(7217..8617)
                     /locus_tag="ACHLIF_003936"
     CDS             complement(7217..8617)
                     /locus_tag="ACHLIF_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO9607854.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLSADVFS
                     ARQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            complement(8614..9492)
                     /locus_tag="ACHLIF_003937"
     CDS             complement(8614..9492)
                     /locus_tag="ACHLIF_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9607855.1"
                     /translation="MMTFNLREQQTRLQARMDELRAEIAFAQKGEKPWPYRSCWMREG
                     RGCCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIIDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITNGGRNWL
                     SFNWPSYRSRIGGVAA"
     gene            complement(9503..10411)
                     /locus_tag="ACHLIF_003938"
     CDS             complement(9503..10411)
                     /locus_tag="ACHLIF_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9607856.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETTRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARI
                     REGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEDFWQHPAAPGGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(10398..10631)
                     /locus_tag="ACHLIF_003939"
     CDS             complement(10398..10631)
                     /locus_tag="ACHLIF_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607857.1"
                     /translation="MIRRVVNSLYHRYNRCPRVGQWFTTSNGHVLRVCLVNTESQMVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGGYASV"
     gene            complement(10628..11290)
                     /locus_tag="ACHLIF_003940"
     CDS             complement(10628..11290)
                     /locus_tag="ACHLIF_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO9607858.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKAGAGGRSPELLEAIYDAPASF
                     LSSMLAHARIMVGWAGEPKGSPVSLSAGTANPVQFTTHEFRSSGGGSFSLLKEAAIMA
                     TVPTSPYLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTY
                     HIYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCIEAFHEAEISLSHTAFRRNQWEV
                     RP"
     gene            complement(11399..12106)
                     /locus_tag="ACHLIF_003941"
     CDS             complement(11399..12106)
                     /locus_tag="ACHLIF_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9607859.1"
                     /translation="MNTAIFNDKASMTSVEIAELVGSQHKDVKRSIERLMGKGVIRSA
                     PMANFEIINNLGLKRNVAAYVFEGEQGKRDSIIVVAQICPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVIHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCCNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            complement(12188..12421)
                     /locus_tag="ACHLIF_003942"
     CDS             complement(12188..12421)
                     /locus_tag="ACHLIF_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9607860.1"
                     /translation="MLRKLIPQNAIARYIGVTPQAVNLWFSKNSVPSRFVLRVCELVE
                     WKVTPHGLRPDLYPYPEDGIPDSLRKSNGITRD"
     gene            12578..13267
                     /locus_tag="ACHLIF_003943"
     CDS             12578..13267
                     /locus_tag="ACHLIF_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO9607861.1"
                     /translation="MKNVKSTENRIAMMLKTKGWSQAELARKLGVSAQSVQYWTTGKT
                     FPRSDKLAQLSVISGYPQSWFLGEDASSTFSSAEKHHTREDSVVFNVLDVEFSCGDGT
                     HVRGDLIDVVRSIELDPEYARRLVGNRAFKNIEIGNARGDSMAPTISPGDLLFLDKTV
                     TYFDGDGIYAFCFDGECYVKRLQKIGSKIMVLSDNPNYQPWSIEKEGMALLYIQSKVI
                     SSVPFNINRFG"
     gene            13415..14116
                     /locus_tag="ACHLIF_003944"
     CDS             13415..14116
                     /locus_tag="ACHLIF_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9607862.1"
                     /translation="MEALQTTPKTCSLNTYNKVLCDDLDLDSFALTIANLLSAVRTFN
                     LLDDTRLKEVGFDVLEFTHEYALAIASTKQQHSIRSGNKIACIRTKREACGLTTTELA
                     RLLDLDEEIILQWESGEFEPTISMLIPLANVLGCDPLSLLSEKNSESAIRVNTTEVHA
                     ESIGARIKSARTKLGLTESDLARMIHTYSDPINDWECGICEVPADHIVPLASALNCDL
                     MWLLTGKSEAKEQQQ"
     gene            14113..14313
                     /locus_tag="ACHLIF_003945"
     CDS             14113..14313
                     /locus_tag="ACHLIF_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607863.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHNLLCAVIVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            14512..14693
                     /locus_tag="ACHLIF_003946"
                     /pseudo
     CDS             14512..14693
                     /locus_tag="ACHLIF_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14699..>15267
                     /locus_tag="ACHLIF_003947"
     CDS             14699..>15267
                     /locus_tag="ACHLIF_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607864.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPGPIRFALQQYIEWNLIQKYGEAEGMGRALFLYQKMLITYPDKGF
                     IVSDMGREGLELLLDEMINDLNTHGMPEG"
BASE COUNT         3948 a         4037 c         3445 g         3837 t
ORIGIN      
        1 gcttccttgg tcagcttcac tgccttacct ccggttccct ccgcatcgct gaccacttct
       61 gccctgccgc cactggctga ccatccctgt actttcaggc ttccctctga ctcgctggca
      121 aggtaagaga gcagtgttgt gacgcctgtg gcttctgcac cggaagacga tgacgggcgc
      181 acctctgata ctgtcgatga tgcccccgcg tttagcgcca ctcttcccgc atggcgcaaa
      241 atcgccgttg ccagacggtc ggaaataatc ccgcggcgag cccatgaact gaaatggctc
      301 gccctgtcct gtgacgtcca ggtggctgag ctgtcacgcc atttcgaacc gtaatacccg
      361 atacccggaa tgtccgggtc ttcttccggt ttgttcgtcg gcacattcac cccgttctca
      421 tcggtcatga acggtacgaa atggatattc ttttccgttt tgtttttgta gctgccgtac
      481 accgtctggt acgaggattc gttcttctgc ttccagaaat acgtcgtatc tccacagatc
      541 cagggaacac cgccagcaga gccaccaacg cactgacctg ccatatccgt caggtctgca
      601 cggaatttat caaccagcgc accaaactgt gctgcgtgat ttccgggcgt gccgtcaaaa
      661 tcaaattccc cctgcatcca caccacggca aacagcacat ttttcgggtt cttcttcagt
      721 gctgcttttg ttcgaccgat aaggtcctta tacagcggct tgtccacacc ccagcgggtt
      781 gaattctccg aggcaccact cgcgtcactg tatgtgccat cagctccggt ggtgaacgct
      841 gaaccaccac gacagcacgg aaccagcaga atgcccgcat tcgccggtat aaacggcagc
      901 agttttttgg cgatatgcag cccctgcccc acggtaccgt actgcccttt tgacaggtcc
      961 gctttcggat ggttaagacg gctcatgtcc tgcacatcat gcagacaatg gtccgccgga
     1021 atgatgtcgt tatatttgca tgctgcaccg cccggtgtca ccgtactgcg gcgcgccagc
     1081 tgcttaatac gcgggtccgg acggtcatat gtctccggca gcggaagacc ttcaccatac
     1141 gacatgccat ttgactgccc tgccagaacc acaacaaagt aatactccgg gtcgctggtg
     1201 gcactgacag ccaccgcctt accctcatta ccggtcaccg ccactggtgt ggtgacatca
     1261 ccttccgccg caatggcctg catcagggta taaggcgtga tggccaccgg actgccaaat
     1321 ggctgccagc cctccttcag tttttgtgtc agtcgttcag caaggtctga cggcgatgcc
     1381 gccctgacca catcgtagtg tttaaatgtc ataacctctc ctgccgccag caaagtaatt
     1441 atccggaata cacaatcatg taacacataa aaaacacgct ataaaaaaac catgtctctt
     1501 atataacaaa aaaacgccga caatttaaaa tgtcctctta ttttaaacaa agaataaacg
     1561 acaccagaaa aacaactcag gctgacagat tgctttcaaa acatcactta taatttatgg
     1621 catcatttaa aaccttaaga aaataaggaa agcaaatgaa ctggatagtg attgatacgg
     1681 taatccagcc agcatgtgac atatcatttt cagccatatg gtgtaacata aaattaatat
     1741 tatggtatca gtctgatatc ttcctccctc cgggctgtat ttttacacca acccggacag
     1801 gcgtaatact caataataaa gagctccccg tcaccattta caacgtgacc ccatttaata
     1861 aaaacgtctg gaatttaatt aaaagcagcc aggaatgccc tgcaaacaca ggaaatatca
     1921 cagataagtg ttttaatcat cgctgcattc tggaaatatg cccatacggg cgaaaataac
     1981 agaatcattc agataaaaag cccctccgga gaggggctga agccgcattt ctgtatcacc
     2041 atgaacatgg tgccgggtgc ctcccggtga gttcagtccg gtgtccctga acccgcgtat
     2101 ctcgatccag gttgtcgtca gagatgacac cttatacacc agtcgcccct ccgcacaggg
     2161 ggattcacca tgcgaaattt ttttaacaaa tgctcagtct gacaggcaac tgtcaactga
     2221 ctgaattgtg acacagatta cacttgttac ccacatacca cgaatcaggt tatgcctcag
     2281 tcattattaa actgcacttc agcaaatccg gagcctgatt cacaggtact ggatttgatt
     2341 gtgacagtca ttcctgtcaa ctgagcactt tgcagtaacg gttgcagatt ccagcgactg
     2401 gtccagtatt ctttcccgtc aaccttcact gtaaatgtgt catcctcatt atacttggaa
     2461 aactcaattt tacctttagc acaatccgcc gccattgcat taacagaaac taatgcaaat
     2521 aaaaccgcca taaacatctt cttcatactt aactccttta tttacccgtt gtatataaaa
     2581 actgtgactt tctgttcaga aacgctgcag ctgtattact ttcccataat gtattgttta
     2641 tttttataac gggcctgtcg ccagttatct gacattctgg ttgactctct tcattcacgg
     2701 cgcgaacaga acgcgccccc tgatgatggc aattcagtat aacggccaca gtacccagta
     2761 tcgctgatat attattaaag gatattctcc ccactctgac accatcctct ccccgatact
     2821 ccggaagcac attgctgatt cgcccccagt tcagagtgag gtccacgtct cccggcgtca
     2881 tcgtatacac aggagcagtt tcagacagtg cctgacgaaa ttctctctgt atctgcctga
     2941 agcgtaaggc ttctgccgtg acagtgacaa aacgcagaac tgctctggat gcatctctgg
     3001 tcattgtatt accactgaac tccattaacg ccagatatga tgaaaccagt gagtgacgac
     3061 tgatttgcat tccggaacgt tccagcgctg cgacacgttg cagagtggta taactgctgt
     3121 ccgttgtcat ggaaaccgtt gtcacatcgg gcactgatat atgtgtaaaa tctgaaaaac
     3181 ggtagaaagt atttgttgcc gtattaacga acccggccac atataaatta ttttgctcaa
     3241 taatcagacg aagatggtca aaacgcgcct gatagacatc aagccctcgt atatccacag
     3301 caaaataact gcccggcggg gtgtggttaa taacagacac cgatgtggtc ccctgagata
     3361 tatgttcaag aggggtcgat atctctgtcc gtatactatt taacgaagag acataacttt
     3421 gttgagtcga aaagtctatc gtaaattccc gggaatagga taccgaagaa aaacccagta
     3481 acaggcacag tacccattta aataatatac acttcatata caggtgttcc ttttggctga
     3541 agtaatcagc accagacccg gcgcagatat aaaaaaggcc cgcaaaagcg agcctggtaa
     3601 ataaatatgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga aggcaattgc
     3661 tctgtccggc tgagctaaca acgctgaata ccgataatgg accgccatcg aggactcgaa
     3721 ccccgcgcag ccagcttcga aggctggcgc tctgtcccga tgagctaatg gcggtatgtg
     3781 atatggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc gctcgctctc
     3841 accactgagc taaagggccg ggcgcaggat aataacggta cgtaactaat cctgcaatat
     3901 catccgttct gactggctaa atcctgaact tccctgaccg tctgctcaaa acgttcagtc
     3961 tccagctcaa cgccagttgc acgacgcccc agcgccagtg cagctttgac tgtcgaaccc
     4021 gaccccatga aaaaatctgc aaccaggtca cccggacgac tgctcgcgct gattatctgc
     4081 tgcagcattt ctgccggttt ttcgcacgga tgtttcccgg gatagtactg caccggttta
     4141 tgcgtccaca catccgtgta cggcacctgc gccgtcacgc caaaataccg ccgcagatgc
     4201 ttatattcac tctgcagttc cgcatactgc cggttcagtg aagtatacgt ctccagcagc
     4261 tggtggtggg gcttttccag ttcaccgcgc tgatgcttct cttctgccac ccgggcaaac
     4321 agcgactgta atttcagata atcgctttcg ttcggtagct gccactgact ggcactgaac
     4381 cagtgcgaca ccatgttttt ctttcctgtg gcatccacta tctgttttgc cgttatcccc
     4441 agggcagcac gcgcatcacg aaagtaagca atcagcggag ccatcacatg ctgtttcagt
     4501 gccctgccct tcgcctcata cccggcatct ttcggacgat acggcccctg ataatgttcc
     4561 gcgaacagaa tgcgctctgt ggcggggaaa tacgccctca ggctttcctt gttgcacccg
     4621 ttccagcgtc cggacggctt cgcccagata atatggttca gcacactgaa gcgttcacgc
     4681 atcatgattt caatgtcaga tgccaggcga tggccacaga acaggtaaag acttccggca
     4741 ggtttcagca cccgccagaa ctgcgccaga cactggtcca gccatttcag gtaatcctca
     4801 tcacccgtcc actggttatc ccagccctca ggcttcactt taaagtacgg cgggtccgtg
     4861 actatcaggt caacagaatt ttcgggtaac gaccggataa attccaggca gtcggcgttg
     4921 attaactcac aactggatat ttttacagta ttaaacatgg atcattaagc ctgtctctga
     4981 taggctcata ccgcttttgc gcaaagcaga tgggcctgag gtttgcttgt gaccccaacg
     5041 catgagcaga tggctggtga gtgcccctaa cacccaccag ccgcccattt accacaaata
     5101 aaaaagcctt caggactgaa ggcgtctgta acaaccaaac tgatagtctg ccagacccgc
     5161 cataacaagc tgggtcagta ttagctggca gcgttcgcgt gaaaggtacg tattctgtgc
     5221 aatctccccg actgtcgccg gtgcggtggc acttaattca ttaaacacca ctctggcggt
     5281 ttctgtcata tcctgctgtt tcagcatgtc tttttccctt ttccggttaa cgtgacacac
     5341 caataactct tgtcgaaaaa gccagcaagc tgaaagaccg gtattcaccg ccaccagcgc
     5401 gtttaacgta ctggtccgat ttcaggcata aaaaacccgc ctgacggcgg atttaagctg
     5461 tgtggcaaag taaccactct taacatactg acatactttt tgcggaccgc actaatcatt
     5521 ttttactttt ttagcagcca gtcgtccatc tccagtctta cccccagcac agacaaacat
     5581 ccgtcaataa acccttcggc tatctgcatc tcaattcgta ttgctttttc gcttttcttt
     5641 ctcgtcctgg ctatctgtct ttttgatatt cgcaacaaat aatgagcaat gagaagcgaa
     5701 tactcctcag gttttttctg cttcagacgt gcaagacagt tttcaatgat aagtccgtca
     5761 tcatcgcagc aggctggacg tggtttagtg gtagatggta aaaggccttt gaatccggca
     5821 gcgatcggag aatagtccac cccggtgtta ccacttgccg cccatgcccc ccagcgttca
     5881 agaaccatct gaatatcacg catcaacttt ctccacaaaa atcaggacag cacaccaatc
     5941 gccagtgcgc gatcgataaa acgaaatatc agctccagtt gggaaccata cttctcttca
     6001 aatgccacgg tatccgcatg cagttcgtca tggtgttttc tgcacaaagg caacacaaaa
     6061 aggtcatgcg cttttgtacc cattccaccc tgaccatggc caatcaggtg atgtggatcg
     6121 tcggctggct taccacaaca cgcgcaaggc tgtgtcttaa cccagcgcgt gtacttttca
     6181 ttaacccagc gacgacgttt ggggcgtaac ataaaagact ccggcgactc cggatccact
     6241 ttcagcgcca gcaccttttt cgctttatcc tggataatgc tggtggcagg aaccgaaggc
     6301 acaaggtcac tctcccgggt gacagacggc aaaacaggct tcggtaatct cagggcctta
     6361 cgggctgcac tttccggtaa ggcatccgcc aggtcattac gaaccagcca ccagcacagt
     6421 tcaggcattg tcacaacgtg actgtcatca aaaccgagat cacgacggac tacggacaac
     6481 acccagcggg cacagttatc cgttgccatt gactccagac gttccgtgaa ctgatcgcga
     6541 agcaggttat cgcagtgcca gcacagacgg attgcgcccg gcgcgtgtcg cattgtggtc
     6601 atgttctcgc tgtgccatcc ggaatgaggc cactggcagc ctttttcacg aagtaaccag
     6661 ctctcaaggc attccacgcc accagcacga cggatcaccg cctcatggcg gaacacggcc
     6721 cgaacggcag gatcatccgc cagcggttgt gatgctgccg gaacggcacc actggcaaaa
     6781 gatgaataac gttccggctc aggctccagc aggacacgcc cctgcataaa caggggcatc
     6841 agctctgaac cgggtctgaa caagacgatc cccatacgcg gggcaatttc aggggtcagc
     6901 agtgctctca cggtcacctc agcgaacggt attgcatgaa cgcaggagaa aaaattcagc
     6961 catcacgcag taaactcttt caccagcgtt tcaaactggc ttacctggcc ttccagttcc
     7021 gccacgcaat ccaccagctc atccaccgcc ttttgtgtgc gatgttttgc ctgcagcaga
     7081 tcacgaagcg ccggagtaag ctgcttgcgg agcgtatctt ttgccacgct cgttttttcc
     7141 atctgttcag cacaacgaag catctcctgc gcctgccgac gaagttgttc cggtgaaaca
     7201 gtggtggttc tgttgttcaa aataaacgct ccgttttact acccgacatg cggttattgc
     7261 tgtatctgcg cggattgccc ggcgtcatgg gagtggaaag aacccgggca ctctcctggt
     7321 ccacaggcag aaaatgcccg ttatgaaaac gccggtaaat ggtacccagc gtgccattac
     7381 gctgtttcgt gatgttgatt tcagctatgc ctctcgcctg tgtctccggg ttgtacacct
     7441 catccctgta aagcatcaga atgatgtctg catccgcctc tatttcccct gagtttttca
     7501 ggtccgagtt catggggcgt ttattgggtc tggattccac gccacgggag agctggctca
     7561 gagcaatcag cggaaaaccg ccggattttg ccaggctttt aagtcccttt gagatttccc
     7621 ccacagcaag gtcgtgacgc cccgtgctgc gggttttaat caggccgagg taatcgacca
     7681 ccaccagcgc cgtttccggg tgtttcatcc ggtggtgcct cgtggttgca catatctcat
     7741 caatggtcag gtttgcctgg tccaccatcc agatattacg ccccgtcatt cgtcccacgc
     7801 cctgtgagaa acgcgcccag tcttcatctt caaaacgggc aacagactta agacgggata
     7861 ccggcattcc cccggcagca gacaccatac gttcaccaat ctgaatgttc gccatctcca
     7921 tggtgaacag aagcacgcca tgcccctgct cagtcacctt gtcgatgata tccagcgcaa
     7981 gttcggtttt ccccatcgaa ggacgggccg caatgaatac caggtcgcct ggctccatac
     8041 cgcccgtttt tgcgtccagt tcatcaatac cggtcatcag cgccctggat ttctccagtc
     8101 cctgattgcg gcattcaaca cggtcgacca cttccggaag gacatcatca atgtgaaccg
     8161 gctgaatgac gccctttccg gtcgacagtg aggccatcat gttctgcgca tccttcaggg
     8221 catcctcggc tgcttcacag gtatgcgcat cccgtaaatt ctgtaatgct tcagtcagtg
     8281 ttttttctgc atcgcgcagt gcggcattac gccgcaacgc tgcgacatag tgctccagtg
     8341 aagacttcac ccaggttttg cgtccggtgt cggtaatcac cggggcaagt tccggcatct
     8401 cattgcacag cagtacgggg tcaatgacgc cggatacacg tgcctgtctg caaattcccg
     8461 cgtaaatatc ccggtactgt cgtgcagaaa aaacgtccgc cgacaatgtg gccagaatat
     8521 ccatcacttc cggatcagcc ccacgcagaa aaaacgcgcc aatgacagcg ccttccaggt
     8581 catcgttacg ccatgccggg gtgttctggc tggtcatgcg gcaacacctc cgatacgaga
     8641 acggtagctg ggccagttaa acgacaacca gttgcgcccg ccattggtga tcctgtcggc
     8701 aatccgggga ctgatgaacg cccacaattc ttccggtgaa aggttgctga tcaagatagt
     8761 tggcaaaata ccctcatacc gggcattgat aatttcctgc aaaatggcca tttcagccgc
     8821 actgccaaac tgaacgccga cttcgtcgat gatcagcaaa tccagtgacg cataatgctc
     8881 aatgacgtca tccgctgttt tttcactgtc attccgccag cagtttttca cagcacgggt
     8941 aaggcgcatc acgtcggtga tctccacact ggccagatag ttacggatga tgtgttttgc
     9001 cattgatacc gccagatgat ttttcccggt accgcaactg ccggtcataa caagactggt
     9061 accgttctcc agcatatctg gccagttctc cgcatagcgg cgacaggccg caagatttct
     9121 ggctgcgtca ggattaacct ccagataatt atcaaactcg cagtcccgaa aacgcagagc
     9181 aattccggcg ttatcagtca gttcttccgc cttgagggac gacagctcca tggtcaaatc
     9241 gttggcctca gctatcaagc agtcagggca gcatgaaatt ttttctctgt cctcgccatt
     9301 acgatcgctc cacaccagaa tatgtgtgtg gtattcgcca tgtttttcgc aacacccgcg
     9361 cccttcacgc atccagcagg aacgataagg ccatggtttt tcgcccttct gagcaaatgc
     9421 aatctctgcc cgtaactcat ccatccgcgc ctgtagtctt gtttgttgtt cacgcaggtt
     9481 aaacgtcatc atcgctgtca cctcagaatg tcaatttgtc actggattta ccgaatttgt
     9541 cagacatggc tcccaggcca gccaggacat cgacctgtcg ctgtcgccca cctccgggag
     9601 ctgctggatg ttgccagaaa tcttcgaagt gacgatcggg tccaaagaac gtcgacgcct
     9661 gcttcacgaa ctgggtgccg gtatttcctg agacacgcac ccaggcagca tagcgtttca
     9721 caccgttgag catggtttct ggtgtcacac cttccctgat tcgggctttc caggctttga
     9781 aggctgctga cttggaatta ccaccagcac gtttgggata ttcctgccag gcctgttcaa
     9841 attccggtga atattcctgt cgggcagaac gcgctggtgc agacgcgtca gcggatgcgc
     9901 caatagtgtt tttactctct gtagtattct ctgaagtaat ctctgttgta ttctctgtaa
     9961 gatcgaaatt ggttttccct tctccgcggc gagtggtttc ccgtgtccgc ggtgaaggct
    10021 ttccctcctc cgcgaaattg ggttttacag tttcccgaaa acgggtttcc ccatttcggg
    10081 aaaactgatt gttttcattg ataatttcat taaggcgctc acaatctata cggtagaaca
    10141 ttttgtgctc aagacgcttg ttggtttcaa ccaaaatgcc tctggacaca agatgcttac
    10201 gcgctacagc ctgttgttca aatgtaagtc cagtttcgtg ttgtatctct tcacgcgttt
    10261 tatgtacgcc ttccgctgca tgtgctttat cctgccagta aaaaatctga ccaaagaaaa
    10321 taacagcgtg cggacttccc atgtatttaa cgagcccagg gtaataagca accggatgcc
    10381 caaaatcgag cagaagatca gacggacgca tagccacctc ccagacgctt aaacattttc
    10441 ccggactgaa aagccaccag cggataactc agggtatgag tacgtccctg aacctggcag
    10501 acaaccatct ggctttctgt attgaccagg caaacccgca gaacgtggcc gttgctggtg
    10561 gtgaaccact gccccacacg ggggcaacgg ttgtatcggt gatacaggga attaacgacg
    10621 cggcgaatca tgggcgcacc tcccattgat tacggcggaa agcggtgtga ctgaggctga
    10681 tttctgcctc atggaatgct tcaatgcagc tctcgtagta ccgcattgtg cgtagactta
    10741 acccaagctg aagcatcatc agaccatcaa gggtgatgta ataaccacgc agggagtcac
    10801 cgtagatgtg ataagtaccc ggtatgaaat tgcgggtaaa aaactcgcgt gagcagttca
    10861 gatactcgat tttgtcgacg atgttctggt gcatgcgctt aaagtggcag gcaacatgca
    10921 gggagaaaat aacggccttg ccgttgacaa cttcaatttt caggtatggg gaagttggga
    10981 ctgtagccat gatggcagcc tccttgagca gtgaaaaact tccaccaccg gagctgcgaa
    11041 actcatgggt ggtgaactga acagggttcg cagtaccggc gctcaaggaa accggcgagc
    11101 ctttcggctc ccctgcccag cccaccataa ttctggcgtg cgcgagcatg gacgataaaa
    11161 aagacgctgg cgcgtcgtat atcgcctcga gcaattccgg gctgcgaccc ccggcacccg
    11221 ctttataagg tgcggagaca gtgtaacgtc ccgaaattgc agaatcaata tttggtcttg
    11281 aaatgatcat atagctgctg atatctttag aactgttctt ggatgtttcg gagccgtttt
    11341 atgcgaaaca gctccccgtt attgatgttg agtgagccgg gttactcccg gctttttttc
    11401 accgctgcca accaataacc tgaaataacc ccattttcgg gtgataccag cgagtccctc
    11461 gcggttctgc ttcctccata acccgataaa aagcagccat aaacggttcc acagcaacaa
    11521 ttgcgcgacg tgacaacaat ccgtccggcg tcatgaactc atgtgtgtct gtaggaattt
    11581 gataggcgtt caccagattg cagcatttat catctgacaa accggttttt gctttcagtt
    11641 ggcgatatcc ggcatagccc tcacgaatag tgcccttttt aatttgctca actgtttcag
    11701 caacgtggat gactttttct tccacctgag tgatccgttt ctgttggcga acggcttcaa
    11761 gagccatcgc ggcaaccatt tcgatttcgc tcattggctt acggatctgt tcttccagtt
    11821 cacgccagcg gtctaccaag cgggcggtga attcagggca aatctgcgcg acaacaatga
    11881 tactgtcgcg tttgccttgt tcaccttcga atacgtaagc agctacattt cttttcaatc
    11941 ctaagttgtt gattatttcg aaattcgcca ttggcgcact tcggataact cccttcccca
    12001 tgagtctttc aatgctgcgt ttaacatctt tgtgttggct gcccaccagc tctgcaatct
    12061 caacgctggt catggatgct ttatcgttaa aaattgcggt gttcatctga ttgctccttg
    12121 aggatgacat ttcagaactc acgccaaaac gtattcatcg tgaattactg cttgtgacag
    12181 tcagtcttta atctctggta ataccgtttg attttcgtaa cgaatcagga attccatctt
    12241 cgggataagg gtatagatca ggtcttaacc catgcggagt aaccttccat tcaaccaatt
    12301 cacacacccg taaaacaaaa cgagaaggaa cggagttttt agaaaaccac agattcaccg
    12361 cttgtggcgt aacaccgatg tatctcgcta tggcgttttg agggatcaat ttacgcaaca
    12421 tgtcgtaatc attcagtttt atcacagcgc agctccaaca ttaactttac aaatcaagaa
    12481 tacatcaaga ataaattaac atgcaagttt caaaaggatc gaatacacta aaatcaagtt
    12541 aatatttatg tgtataaagc ttcgacagga actcaccatg aagaatgtaa aaagcacaga
    12601 aaatcggata gcaatgatgc tgaaaacgaa aggctggagt caggctgaac tagcccgtaa
    12661 gctgggtgta agcgcgcaat cagtacaata ctggactaca ggaaagacgt ttcccagaag
    12721 cgataaactt gcacagctat cagtaattag tggttatcca caatcctggt ttttgggtga
    12781 agatgcctca tcgacattct cttcagctga aaaacaccat acaagagaag acagcgttgt
    12841 gttcaatgta ctggatgttg aattcagttg cggcgacgga actcatgtac ggggagatct
    12901 aattgatgta gtacgctcaa tagaacttga tcctgaatat gcccgccgcc tggtcggtaa
    12961 cagagcattc aagaacatag aaatcggtaa cgccagaggg gacagcatgg ccccaacaat
    13021 ctccccggga gacttactgt ttttagataa aacagtaact tactttgacg gcgatggcat
    13081 ttatgcattt tgctttgatg gcgaatgtta cgtcaaacga cttcaaaaaa ttggaagcaa
    13141 gatcatggtc ttatcagaca atcccaacta tcagccatgg agcatcgaaa aagagggaat
    13201 ggcgctgctc tatatccagt caaaagtaat ctcatctgta ccattcaata tcaacagatt
    13261 tggttagtta ttgattttaa attaaattat tggtcatcca tcacaaaaaa atcaagttta
    13321 tcaattttta attgacagtc gttttcctga cacataatat ctcaccatca attatatatt
    13381 gattaacttc aacttagaat tgcatggtga tgatatggaa gccttacaaa caacaccaaa
    13441 aacatgtagc ctcaatactt acaacaaggt tttatgtgat gatttagatc tcgattcttt
    13501 tgcattaacc atcgcaaacc tgctcagtgc tgttcgcacc ttcaacctcc tagatgatac
    13561 gcgattaaaa gaggttgggt ttgatgtgct agaatttact catgaatatg ctttagcgat
    13621 cgcatcgaca aaacaacaac attctatccg atcaggcaac aagatagcct gcatacgcac
    13681 caaacgtgaa gcctgcggct tgacgactac cgaactagcc agactactcg atcttgacga
    13741 agagattatt ctgcaatggg agagtggaga gttcgagcca accatcagca tgcttatccc
    13801 cctggcaaac gtcctgggat gcgatccgct ttctctacta agtgaaaaaa acagcgagtc
    13861 agctattcgt gtaaatacga ctgaagtcca tgcggaaagt attggcgcac gcatcaaaag
    13921 cgcccgtaca aagctgggat taaccgaatc tgatcttgcc cgcatgattc atacctacag
    13981 tgaccccata aacgactggg aatgcggcat ctgtgaagtt cctgctgatc atatagtacc
    14041 actggccagt gcgcttaatt gtgacctgat gtggttgtta acgggaaaat cagaagcaaa
    14101 ggagcagcaa caatgactgg caatatccat gataagtatg aaggcttatg cctggcaccg
    14161 gattcctttg caaacaatat ccataattta ttatgcgcag ttatcgtatt acaaatgtca
    14221 gacaacgacg caataaaaag aacaggtgat gaagttcttg aatttgcacg ttgctatgct
    14281 gaagcagcag ctgaaaaaga actatccagt taaatagaac aagtcatctc cagatactat
    14341 attacggctt aatcgccggg gattatcaca cacttaatcc acaggaggtt ttattatgac
    14401 ttttataaaa cataaggcat cacacaaaac agcctgcctt attgcacaac acgggaaaaa
    14461 tcacatgcat attgcctgct tgtttctgcg taaagcatac gggagataat aatgcatcag
    14521 aaaacagcag aacacgaaca aaccagagta ttgctgacca tcaaaaacgg gaaagtaata
    14581 ttaattcgtc atgttcatga cgatgaattt gtagaaatct ttcaacattc ctgtttattg
    14641 cagaaaaggc aggatatgac gttattgcac cagcagatga agatgaggaa taaatttcat
    14701 gcaatacgct gaattccagg ctgaagcaac agccaatggt atacaaacag gcagtatgac
    14761 tattgattat cacgacgcca tacgccgtct ggatgctgga gaattcgata ctcctaatgt
    14821 gcgaggttta cgtatccttc agtgtctcgc gcaagctgac gaagcaggat tactgggaaa
    14881 acttccggtt gagatgaagg ttgcccagtg gcgatggttg tatgtgacga cattcatcaa
    14941 cgaagaagaa gacaagaacg gcacaattga tgtcccgaat gaacacggaa caacagatcg
    15001 cgccgtaata tataacggga agcatggggt tatgacgata tatcccggcc ccattcggtt
    15061 tgccttacag cagtatattg aatggaattt aattcaaaaa tacggcgagg cagaaggaat
    15121 gggtagagcg ctgtttcttt atcagaaaat gctcattaca tacccagata aaggtttcat
    15181 tgtttcagac atggggcgag aagggcttga actccttctg gatgaaatga ttaacgacct
    15241 gaatactcat ggtatgccag aaggcca
//
LOCUS       DAZGOB010000122        15179 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGOB010000122 DAZGOB010000000
VERSION     DAZGOB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.122"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            104..346
                     /gene="yjbE"
                     /locus_tag="ACHLIF_003948"
     CDS             104..346
                     /gene="yjbE"
                     /locus_tag="ACHLIF_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO9607865.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            460..1098
                     /locus_tag="ACHLIF_003949"
     CDS             460..1098
                     /locus_tag="ACHLIF_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO9607866.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1095..1832
                     /locus_tag="ACHLIF_003950"
     CDS             1095..1832
                     /locus_tag="ACHLIF_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO9607867.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1832..3928
                     /locus_tag="ACHLIF_003951"
     CDS             1832..3928
                     /locus_tag="ACHLIF_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO9607868.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKVAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3975..4253)
                     /locus_tag="ACHLIF_003952"
     CDS             complement(3975..4253)
                     /locus_tag="ACHLIF_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607869.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4254..4397
                     /locus_tag="ACHLIF_003953"
     CDS             4254..4397
                     /locus_tag="ACHLIF_003953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607870.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4523..4933
                     /gene="psiE"
                     /locus_tag="ACHLIF_003954"
     CDS             4523..4933
                     /gene="psiE"
                     /locus_tag="ACHLIF_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO9607871.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4977..6452)
                     /gene="xylE"
                     /locus_tag="ACHLIF_003955"
     CDS             complement(4977..6452)
                     /gene="xylE"
                     /locus_tag="ACHLIF_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO9607872.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLHTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDATWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNALAAQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVTHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6824..7714)
                     /gene="malG"
                     /locus_tag="ACHLIF_003956"
     CDS             complement(6824..7714)
                     /gene="malG"
                     /locus_tag="ACHLIF_003956"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO9607873.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7729..9273)
                     /gene="malF"
                     /locus_tag="ACHLIF_003957"
     CDS             complement(7729..9273)
                     /gene="malF"
                     /locus_tag="ACHLIF_003957"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO9607874.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9427..10617)
                     /gene="malE"
                     /locus_tag="ACHLIF_003958"
     CDS             complement(9427..10617)
                     /gene="malE"
                     /locus_tag="ACHLIF_003958"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO9607875.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10982..12097
                     /gene="malK"
                     /locus_tag="ACHLIF_003959"
     CDS             10982..12097
                     /gene="malK"
                     /locus_tag="ACHLIF_003959"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO9607876.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12169..13509
                     /gene="lamB"
                     /locus_tag="ACHLIF_003960"
     CDS             12169..13509
                     /gene="lamB"
                     /locus_tag="ACHLIF_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO9607877.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13752..14672
                     /gene="malM"
                     /locus_tag="ACHLIF_003961"
     CDS             13752..14672
                     /gene="malM"
                     /locus_tag="ACHLIF_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO9607878.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14901..15179
                     /locus_tag="ACHLIF_003962"
                     /pseudo
     CDS             14901..15179
                     /locus_tag="ACHLIF_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3791 a         3881 c         3975 g         3532 t
ORIGIN      
        1 ctaattcccc gcgccgtaat tcgcatgctt tagttgtgta tactcgatcc cgcccgaaat
       61 gtttttgggt aaatctccat tcattcaatg aagggaaatt gttatgaaaa aagttctgta
      121 tggcattttt gccatatctg cgcttgcggc gacttctgcg tgggctgcac ctgtacaggt
      181 gggcgaagcg gcagggtcgg cagcaacgtc ggtttcggcg gggagttcct ccgctaccag
      241 cgtcagcacc gtaagctcgg cggtgggtgt cgcgctcgcg gcaaccggtg gcggtgatgg
      301 ttctaatacc gggaccacca caaccacgac caccagtacc cagtaataaa gtatgtatcc
      361 ccaaaataat ttgagtcatt gcatctgtgg cttgaagtat gaagggattt accataacca
      421 cactccggtg tggttattct gcccctctgg agaagagtcg tgaagcgacc tgcactcatt
      481 cttatctgcc tgctattaca ggcctgttca gccacgacta aagagctggg caattcactg
      541 tgggacagtc tgttcggcac gccaggcgta cagctgacgg acgatgatat tcaaaatatg
      601 ccctacgcca gccagtacat gcagcttaat ggcgggccgc aattgtttgt ggtgctggcc
      661 ttcgctgaag acggacaaca aaaatgggtc actcaggatc aagccactct tgtcacacaa
      721 catggtcgtc tggtgaagac tttgcttggc ggcgacaacc tgattgaagt gaataacctt
      781 gccgccgacc cgctgattaa acccgcacaa attgttgatg gtgcaacatg gacgcgcacg
      841 atgggctgga ccgagtacca gcaggtacgc tacgccaccg cacgctcagt cttcaaatgg
      901 gatggcaccg ataccgtcaa agtcggcagc gatgaaaccc tggttcgcgt gctggacgaa
      961 gaagtctcca ccgaccaggc gcgctggcat aaccgctact ggatcgacag cgaagggcaa
     1021 attcgccagt cggaacagta tctcggcgcg gattatttcc cggtgaaaac cactctcatc
     1081 aaggcggcaa aacaatgatt aaacaaactt ttgtcgcgtt gcttttgagt gtgggagcgt
     1141 catcggtctt cgcggcagga accgtcaagg tgttcagcaa tggcagcagt gaggccaaaa
     1201 cactaacagg cgcagagcat ttaatcgatc tggtaggcca accgcggctg gcaaacagct
     1261 ggtggcccgg tgcggtgatt agcgaagagc tggcaacggc ggcagcattg cgtcagcagc
     1321 aggcgttgct gacacggctg gcagaacagg gcgcagattc cagcaccgac gatgccgccg
     1381 ccattaacgc cttacgccag caaatccagg cattgaaagt gacaggcagg caaaaaatca
     1441 atcttgatcc cgatatcgtc cgcgttgccg aacgcggtaa cccgccgttg cagggcaact
     1501 acacgctgtg ggtcggacca ccgccgtcca cggtcacgtt gttcgggctt atcagccatc
     1561 ctggcaatca gccattcact ccaggtcgcg acgtggcgag ctatctctcc gggcagaacc
     1621 tgcttagcgg tgcggatcgc agctacgcgt gggtcgttta cccggacgga cgcacgcaaa
     1681 aagcgccggt ggcttactgg aacaagcgtc acgtagagcc gatgcccggc agcattattt
     1741 atgttggcct cgcggactcc gtctggagtg agacgcctga tgcccttaac gccgacattc
     1801 ttcagactct gacgcagcgg ataccccaat aatgaaaaaa agacatctgc ttagcttact
     1861 ggcgctgggc attagcatgg cttgctacgg cgaaacatat cctgcgccca ttggcccgtc
     1921 gcagtcggat ttcggtggcg taggattatt acaaactccc accgcgcgta tggcacggga
     1981 aggggagttg agtctgaact atcgcgataa cgatcagtac cgttattact cagcttcagt
     2041 gcaactcttc ccgtggctgg aaacaacgct gcgctacacc gacgtgcgca cccggcagta
     2101 cagcagcgtc gaagcgttct ctggcgatca aacgtataaa gataaagcct tcgatctcaa
     2161 actgcgtttg tgggaagaga gttattggct gccgcaagtg gcggttggtg cgcgggatat
     2221 cggcggtacg gggctgtttg atgcggaata tcttgttgcc agcaaagcct gggggccgtt
     2281 cgattttacg ctcggcctgg gctggggata tctgggcacc agcggtaatg tgaaaaatcc
     2341 gctctgttca gccagtgata aatattgcta tcgcgataac agctacaaac aggcgggatc
     2401 tatcgacggt agccagatgt tccacggtcc tgcctcactg tttggcggcg tggaatacca
     2461 gacgccctgg caaccgctgc gcctgaaact ggagtatgaa ggcaataatt atcagcagga
     2521 ttttgccggg aagctggagc aaaaaagtaa gtttaacgtc ggtgcgattt atcgcgttac
     2581 cgattgggcc gacgttaacc ttagctatga acgtggcaac acctttatgt ttggcgttac
     2641 gttgcgcacc aactttaacg atctgcgccc gtcttacaac gataacgccc gcccgcaata
     2701 tcaaccgcag ccgcaggatg ccattttgca gcattcggtg gtggcgaatc agttaacgtt
     2761 gctgaaatac aatgccggac ttgccgatcc gcagatccag gcgaaaggcg atacgctgta
     2821 tgttaccggc gagcaggtga aatatcgtga ttcgcgcgaa gggatcatcc gtgcgaatcg
     2881 gatcgtgatg aacgatctgc cggatgggat caaaacgatc cgcattacag aaaatcgcct
     2941 taacatgccg caggtgacga cggaaaccga tgtcgccagc ttgaaaaatc atctcgccgg
     3001 ggagccgctg ggccatgaaa cgaaggtggc gcaaaaacgc gtcgagccag tggttccgaa
     3061 gtccaccgag cagggatggt atatcgacaa atcacgcttt gatttccata tcgatccggt
     3121 gctgaaccag tcggtcggtg gcccggaaaa cttttacatg tatcagctgg gcgtgatggg
     3181 aacggcagat ttgtggctga cggaccatct gctgaccacc ggcagcctgt ttgcaaatct
     3241 tgccaacaac tacgacaagt ttaactacac caatccgccg caggactcgc acttaccgcg
     3301 cgttcgtacc catgtgcgcg agtatgtgca gaacgatgtc tatgtgaata acctgcaagc
     3361 caactacttc cagcatctgg gcaacggctt ctacggtcag gtctatggag gttatctcga
     3421 aaccatgttt ggcggtgccg gggcagaagt gttgtatcgt ccgctggaca gcaactgggc
     3481 gtttggtctg gatgccaact acgttaaaca gcgcgactgg cgtagtgcaa aagatatgat
     3541 gaaattcacc gactacagcg tgaaaaccgg acatctgacc gcctactgga cgccatcttt
     3601 cgcccaggac gtgttggtga aagcgagcgt cgggcagtat ctggcagggg ataaaggcgg
     3661 cacgctggag atcgccaaac gctttgatag cggcgtggtg gttggcggct atgccacgat
     3721 cacgaatgtt tcgaaagagg agtacggcga aggggacttc accaaaggcg tgtatgtctc
     3781 tgtgccgttg gatctcttct cgtctggccc gacacgcagc cgtgcggcga ttggctggac
     3841 gccgctgacg cgtgacggtg gtcagcaact tgggcgtaag ttccagttgt atgacatgac
     3901 cagcgaccgt agcgtcaatt tccgctaagt catgggaaag gtgccagttt tcgcaatcac
     3961 tggcaccctc attcttaatg gcctgttgag ccatcaggaa cgcagttcac ccccggtttg
     4021 cgatctggtg tgtaaaggcg aaaacattct gtcttcctgt ccgtcgatat cggttgcaga
     4081 tctggttgcc tgagcgactg ggccatcatt gggctggaga cattgctttg cggtaggtcg
     4141 gctttatcag cagtaccgag cggaccagca taagcaggaa gaacagagac tgataacatc
     4201 aaagcagcaa aataaggctt catttttacc acctttatca ggttacgttt catttgttcc
     4261 agaggaacat tgtcgatttt tcgcgcattg ctggtggctg ggaatcacct gaatgggtga
     4321 tttttgaatt accggctttg gtgcggtttg tcttgccgga tgcgccgcca ggcgcggctt
     4381 atccggccta cgggtaggta tatccggctt tggtggaggc gcgctccaaa tccaggttga
     4441 acaaaacata cacaaaaaat atagatctcc gtcacatttt tgcgttatac aggaagctcg
     4501 ccactgtgaa ggaggtactg ctatgacgtc actctctcgt ccgcgcgtgg agtttatctc
     4561 caccattttg cagaccgtac tcaatcttgg cctgttgtgc ctcggcctga ttttggttgt
     4621 cttcctcggc aaagaaacgg tgcatctggc tgatgtgctg ttcgcgccag aacaaaccag
     4681 caaatatgag ctggtagaag ggctggtggt ttactttctc tatttcgaat ttatcgcgct
     4741 gattgtgaag tactttcagt ccggttttca cttcccgcta cgttactttg tctatattgg
     4801 gatcaccgcc attgtgcgct tgatcatcgt cgatcataaa tcgccactgg atgtattgat
     4861 ctactcggca gcgatcctgt tactggtgat caccctgtgg ctgtgtaatt cgaaacggct
     4921 gaagcgggag taaaaagaca gcacgccgaa ctggcgcggc gtgctggaca ggaagattac
     4981 agtgtagcag tttgttgtgt tttcttcgtt tccggttccc agagggcttc cagctcctca
     5041 agggttttac ctttggtttc cgggacaaat ttccacataa atagtgctgc cagaacgccc
     5101 atacaaccgt aaatccagta ggagaaaccg ttgtggaaat gggtcaccag ccaggagttt
     5161 ttgtccatca tcgggaaggt ccaggagacg aagtagttcg ccagccactg ggccgccacc
     5221 gcgattgcca gcgctttacc acgaatagca ttcgggaaga tttccgacag cagtacccag
     5281 cataccggac cccaggacat ggcaaaggca gcaacataga acagcatcga cagtagcgcc
     5341 acaatacccg gtgcctgagt gtaaaacgcg gtaccgaggc taaacatacc gattgccatt
     5401 ccgagcgcgc cgataatttg cagtggctta cgaccaaatt tatccaccgt cataattgcc
     5461 agaacggtga aggtgaggtt gataactccg acaataatgg tctgcaacag cgcgatatcc
     5521 gtgctggccc ccagcgtttt gaacacttcc ggcgcgtagt acaacaccac attgatgccg
     5581 acaaattgct ggaagatgga gagcataacg ccgataacaa tcacgcccac gccgaacatc
     5641 agcagacgac caccggtttt gcgtccatga tccagggagt gtttaatttc ctgcaccgcc
     5701 tgggctgcca aagcgttgcc cataattttg cgcaggatgc cttccgcttg ttcttgctta
     5761 ccgcgtgaca tcagccagcg agggctttcc ggcacggtat aaagcagcat taagaacagc
     5821 agcgcaggga tacattccga agcaaacata taacgccagc cgtcagtatt cagccaggtg
     5881 gcatcgccgg aacgggcaat aaaatagttt acgcagtaaa ctaaaagttg gccgaaaata
     5941 atcgcaaact ggttaaaaga gaccagcttc ccacgaatat gtgcaggagc cagttccgca
     6001 atatacattg gtgagagcat tgaggctaaa ccaacaccaa taccgccaat aatgcgataa
     6061 ataacaaatt ccgggacata acctgccaga taaacaggca ctgtgttgtc cgggtttata
     6121 gaggtaaaac caagttctgg ccaggcagaa cctacaccag aaataaaaaa caggacagca
     6181 gcaatcttaa gtgaatcacg acgaccgaag cggttactgc aataaccacc gagggcaccg
     6241 ccgatgatgc aaccaatcag agcgctggct acgcaaaacc ctaacaggga gttggcagcg
     6301 gattcactta agttttgtgg agcaacaaag acggtatgga gtgactcaac agtaccggaa
     6361 ataacggcgg tgtcgtagcc aaataataaa ccacctaatg tagcgactaa ggtaatcgaa
     6421 aatatataac tggaattata ctgggtattc attcagacct gccttagacc attctgatct
     6481 tttatcagtg cattgatgtt ggaattgtca ggcagttaag gcaattatca ttttttacaa
     6541 tgcgtttacg taatgtcttt tctgtgatct taattgtgat aattatctaa aattgatgaa
     6601 aacaatacta ttgccgtgac tcagagcacg aaagagaatt atcgtaagtg ggaaaacaaa
     6661 taacgtaaaa ataataagct ctatgatggg aatattaacc ggcgaacgat tcagattgca
     6721 gacgaaagaa aaaaaggcgc tccgtggagc gccgaataac agtcacaagt tgggataacg
     6781 taagttgagg gtgcagcggc ataacattgg cagaacaaca tctttaacct ttcacaccac
     6841 ctgccgtcag gccgttgacc agccagcgtt gagccagcaa gaagacgatg gtgatcggta
     6901 atgcagacat cacggcagcg gcggcaaagt caccccacag gtagttttgc gggttgaggt
     6961 attgctgcat ccccacggcc agggtgtagc tgtttacgtc acgcagtaac agcgacgcga
     7021 ccggaacttc agtaatggca gcgataaacg acaagatgaa taccaccgcc agaatcggta
     7081 ctgacagcgg caacaggaca aggcggaagg cctgccacgg tgtcgcacca tccagcgcag
     7141 ccgcttcttc cagcgaacta tcgatggttt cgaaatagcc tttgatggtc cagacatgca
     7201 gcgcaatccc acccagatac gcgaaaatta cgccgccgtg agtattcagg ccaatgaatg
     7261 gaatgtactc acccagacga tcaaacaacg catacaacgc gaccagtgaa agtactgccg
     7321 ggaacatctg gaaaatcagc attcctttca gcagcgtcgc tttgcctgga aagcgcatac
     7381 gggcgaaagc gtaggcgcag gtggtagaga gcgccacaat gccaatcgcg gaaatcccgg
     7441 cgacctttac cgagttccac agccacagca gtaccgggaa tggcggtggc gtaatgcgac
     7501 catcagcctg ttcaacgcta aaacctaacg ccagtttcca gtgatcccag gagatttgct
     7561 ccgggatcag gctaccggtc gcgaagtttc cctgacgcag cgagatagcg acgaccatca
     7621 gcagcgggaa cataatcgcc gcgataaaaa gtagcagtag caggtgagta ataaacaaac
     7681 gtgctttttg cgatttcggt tggaccattg ccatttttgt tatctccctt aatcaaactt
     7741 cattcgcgtg gctttcaggt tcactatcgc cagcgcaccc accagcagga agatcagcgt
     7801 ggcaattgct gctgccagac cgaagtcctg acccccgccg ccttcaaaag cgatgcggta
     7861 ggtgtagtta acaagcaggt cggtataacc ggctggcgtg gtcgtgccaa gacgatccgg
     7921 gccgccgttg gttaacagtt gaatcagcac gaagttgtta aagttaaagg cgaagctggc
     7981 gatcatcagc ggcgtcagcg gtttaatcag cagcggcagc gtaatcttaa agaagttctg
     8041 gaacggacct gcgccatcca ttgctgaggc ttcatacaaa tcgtccggaa tcgctttcag
     8101 caagcccatg cagaggatca tcatgtacgg ataacccagc caggtattga cgataatcag
     8161 catcgtgcgg gcggtggtcg gatcgctgaa ccaggcgggc ttcacgccaa acagcgcgct
     8221 caacatcatg ttgatttcac cgaagctctg gttaaacaac cctttgaaaa tcaagattga
     8281 aatgaacgat ggcaccgcgt agggcagaat cagcaggacg cgatagaccg ctttgccgcg
     8341 caacgcttcc cactgcacca gacacgccag aaccatgccg accgccaccg ttaaaaagac
     8401 agtgatcagc gagaacacca cggtccagac gaaaatggcg aggaacggtt tctgaatgcc
     8461 ttcgtcggtg aagacgcggg taaagttttt ccagccagta gtcacggtgt aaccggggct
     8521 tagcttttca tcgccccagt tgccgtcggc ggtaatggac tggtaaaagc caatttggtt
     8581 attcggacga tatttcacgc cgctctgatt attcgtcaac gtgccgtcac cgtcgagtgt
     8641 gtagagcggc tgcgtgccag aaaactggcg cagggagctc atcatcactt tgttgccatc
     8701 cggcagaatg gcggtaatgt cactcagcgc ctgacgattc tgggtaatca cgcgcagatt
     8761 cgcgcgttcg ccttcgggct gggtggtcgt ttctttcagt tgcagttttt gctcgccgcc
     8821 aaatttaaaa gcgtcggaga ggtaattttt gccggtttcg ccgtcgctga gggctagttg
     8881 ccactcatcg cccgccgggt aaagaccaaa attataggtt ttgcctgctt gccaggagcg
     8941 atctagcaac acttcctgcg cacgttcaaa agtcagctgg ttagtgctgc tgtagttggt
     9001 gaaggcaatg gcgatggtgc agaccagagg gaagaggacg aataatccca ttccagccat
     9061 tcccgggtaa acatagcgcc aggcgtaggc tttacgattg gcgaaaatat acagccccgc
     9121 tgaactcaat atcagcgtgg taatggcgaa caggtattcc ccttgtgcgt acattaaaac
     9181 aacaaggtaa cccaccagca ggccgagcag acctaacact gaccatttca gcgcgtcgct
     9241 ttgccaccaa tgtttctttt taatgacatc catggggttc ttcctcattc caggacggat
     9301 aaggctttca cgccttatcc gacaacaact gcctgatgcg acgctgacgc gtcttatcag
     9361 gcctacatac gtttcggttt tgtaggccgg acaaggcgtt cacgccgcat ccggcatttc
     9421 acagcattac ttggtgatac gagtctgcgc gtctttcagg gcttcatcga cagtctgacg
     9481 accgctggcg gcgttgatca ccgcagtacg cacggcatac cagaaagcgg acatctgcgg
     9541 gatgttcggc atgatttcac ctttctgggc gttttccatg gtggctgcaa tacgtggatc
     9601 tttcgccaac tcttcctcgt aagacttcag cgctacggca cccagcggtt tgtctttatt
     9661 aaccgcttcc agaccttcgt cagtcagcag atagttttcg aggaactctt tcgccagctc
     9721 tttgttcgga ctggcggcgt taataccggc gctcagcacg ccaacgaacg gtttagatgg
     9781 ttgacccttg aaggtcggca gtaccgttac accataattc actttgctgg tgtcgatgtt
     9841 ggaccatgcc cacgggccgt tgatggtcat cgctgtttcg cctttattaa aggcagcttc
     9901 tgcgatggag taatcggtgt ctgcattcat gtgtttgttt ttaatcaggt caaccaggaa
     9961 ggtcagaccc gctttcgcgc cagcgttatc cacgcccacg tctttaatgt cgtatttgcc
    10021 attttcatac ttgaacgcat aacccccgtc agcagcaatc agcggccagg tgaagtacgg
    10081 ttcttgcagg ttgaacatca gcgcgctctt acctttcgct ttcagttctt tatccagcgc
    10141 cgggatctct tcccaggttt ttggtgggtt cggcagcaga tctttgttat aaatcagcga
    10201 taacgcttca acagcgatcg ggtaagcaat cagcttgccg ttgtaacgta cggcatccca
    10261 ggtaaacgga tacagcttgt cctggaacgc tttgtccggg gtgatttcag ccaacaggcc
    10321 agattgagcg tagccaccaa aacggtcgtg cgcccagaag ataatgtcag ggccatcgcc
    10381 agttgccgca acctgaggga atttctcttc cagtttatcc ggatgctcaa cggtgacttt
    10441 aattccggta tctttctcga atttcttacc gacttcagcg agaccgttat agcctttatc
    10501 gccgttaatc cagattacca gtttaccttc ttcgattttg gcgagagccg aggcggaaaa
    10561 catcatcgtc gttaatgcgg ataatgcgag gatgcgtgca cctgttttta ttttcataat
    10621 ctatggtcct tgttggtgaa gtgctcgtga aaacacctaa acggactcta gtttctttat
    10681 acggcaacct ctttccatcc tccttgcccc tacgccccac cgttgctttg tgtgatctct
    10741 gttacagaat tggcggtaat gtggagatgc gcacataaaa tcgccacgat ttttgcaagc
    10801 aacatcacga aattccttac atgacctcgg tttagttcac agaagccgtg ttctcatcct
    10861 cccgcctcct cccccataaa aaagccaggg ggtggaggat ttaagccatc tcctgatgac
    10921 gcatagtcag cccatcatga atgttgctgt cgatgacagg ttgttacaaa gggagaaggg
    10981 catggcgagc gtacagctgc aaaatgtaac gaaagcctgg ggcgaggtcg tggtatcgaa
    11041 agatatcaat ctcgatatcc atgaaggtga attcgtggtg tttgtcggac cgtctggctg
    11101 cggtaaatcg actttactgc gcatgattgc cgggcttgag acgatcacca gcggcgacct
    11161 gttcatcggt gagaaacgga tgaatgacac tccgccagca gaacgcggcg ttggtatggt
    11221 gtttcagtct tacgcgctct atccccacct gtcagtagca gaaaacatgt catttggcct
    11281 gaaactggct ggcgcaaaaa aagaggtgat taaccaacgc gttaaccagg tggcggaagt
    11341 gctgcaactg gcgcatttgc tggatcgcaa accgaaagcg ctctctggcg gtcagcgtca
    11401 gcgtgtggcg attggccgta cgctggtggc cgagccaagc gtatttttgc tcgatgaacc
    11461 gctctccaac ctcgatgctg cactgcgtgt gcaaatgcgt atcgaaatct cccgtctgca
    11521 taaacgcctg ggccgcacaa tgatttacgt cacccacgat caggtcgaag cgatgacgct
    11581 ggccgacaaa atcgtggtgc tggacgcagg tcgcgtggcg caggttggga aaccgctgga
    11641 gctgtaccac tatccggcag accgttttgt cgccggattt atcggttcgc caaagatgaa
    11701 cttcctgccg gtaaaagtga ccgccaccgc aatcgatcaa gtgcaggtgg agctgccgat
    11761 gccaaatcgt cagcaagtct ggctgccagt tgaaagccgt gatgtccagg ttggagccaa
    11821 tatgtcgctg ggtattcgcc cggaacatct actgccgagt gatatcgctg acgtcatcct
    11881 tgagggtgaa gttcaggtcg tcgagcaact cggcaacgaa acccaaatcc atatccagat
    11941 cccttccatt cgtcaaaacc tggtgtaccg ccagaacgac gtggtgttgg tagaagaagg
    12001 tgccacattc gctatcggcc tgccgccaga gcgttgccat ctgttccgtg aggatggcac
    12061 tgcatgtcgt cgactgcata aggagccggg cgtttaagca ccccacaaaa cacacaaagc
    12121 ctgtcacagg tgatgtgaaa aaagaaaagc aatgactcag gagatagaat gatgattact
    12181 ctgcgcaaac ttcctctggc ggttgccgtc gcagcgggcg taatgtctgc tcaggcaatg
    12241 gctgttgatt tccacggcta tgcacgttcc ggtattggct ggacaggtag cggcggtgaa
    12301 caacagtgtt tccagactac cggtgctcaa agtaaatacc gtcttggcaa cgaatgtgaa
    12361 acttatgctg aattaaaatt gggtcaggaa gtgtggaaag agggcgataa gagcttctat
    12421 ttcgacacta acgtggccta ttccgtcgcg caacagaatg actgggaagc taccgatccg
    12481 gccttccgtg aagcaaacgt gcagggtaaa aacctgatcg aatggctgcc aggttccacc
    12541 atctgggcag gtaagcgctt ctaccaacgt catgacgttc atatgatcga cttctactac
    12601 tgggatattt ctggtcctgg tgccggtctg gaaaacatcg atgttggctt cggtaaactc
    12661 tctctggcag caacccgctc ctctgaagct ggtggttctt cctcttttgc cagcaacaat
    12721 atttatgact ataccaacga aaccgcgaac gacgttttcg atgtgcgttt agcgcagatg
    12781 gaaatcaacc cgggcggcac attagaactg ggtgtcgact acggtcgtgc caacctgcgt
    12841 gataactatc gtctggttga tggcgcatcg aaagacggct ggttattcac tgctgaacat
    12901 actcagagtg tcctgaaggg ctttaacaag tttgttgttc agtacgctac tgactcgatg
    12961 acctcgcagg gtaaaggtct gtcgcagggt tctggcgtcg cgtttgataa cgaaaaattt
    13021 gcctacaata tcaacaacaa cggtcacatg ctgcgtatcc tcgaccacgg tgcgatctcc
    13081 atgggcgaca actgggacat gatgtacgtg ggtatgtacc aggatatcaa ctgggataac
    13141 gacaacggca ccaagtggtg gaccgttggt attcgcccga tgtacaagtg gacgccaatc
    13201 atgagcaccg tgatggaaat cggctacgac aacgtcgaat cccagcgcac cggcgacaag
    13261 aacaatcagt acaaaattac cctcgcacaa caatggcagg ctggcgacag catctggtca
    13321 cgcccggcta ttcgtgtctt cgcaacctac gccaagtggg atgagaaatg gggttatgac
    13381 tacaacggcg atagcaaggt taacccgaac tacggcaaag ccgttcctgc tgatttcaac
    13441 ggcggcagct tcggtcgtgg cgacagcgac gagtggacct tcggtgccca gatggaaatc
    13501 tggtggtaat agcaaaacct gggccggata aggcgtttac gccgcattcg gcaaccaacg
    13561 cctgatgcga cgcttgcgcg tcttatcagg ccgacaacgg ctgtcaaatg taggccggat
    13621 aaggcgttta cgccgcatcc ggcataaaaa caggttgtca ttatctgaaa ggggcgaaag
    13681 cccctctgat tatcgggttt agcgcgctat tgcctggcta ccgctgagct ccagattttg
    13741 aggtgaaaac aatgaaaatg aataaaagtc tcatcgccct ctgtttatca gcagggttac
    13801 tggcaagcgc gcctggaatc agccttgccg atgttaacta cgtaccgcaa aacaccagcg
    13861 acgcgccagc cattccatct gctgcgctgc aacaactcac ctggacaccg gtcgatcaat
    13921 ctaaaaccca gaccacccaa ctggcgaccg gcggccaaca actgaacgtt cccggcatca
    13981 gtggtccggt tgctgcgtac agcgtcccgg caaacattgg cgaactgacc ctgacgctga
    14041 ccagcgaagt gaacaaacaa accagcgttt ttgcgccgaa cgtgctgatt cttgatcaga
    14101 acatgacccc atcagccttc ttccccagca gttatttcac ctaccaggaa ccaggcgtga
    14161 tgagtgcaga tcggctggaa ggcgttatgc gcctgacacc ggcgttgggg cagcaaaaac
    14221 tttatgttct ggtctttacc acggaaaaag atctccagca gacgacccaa ctgctcgacc
    14281 cggctaaagc ctatgccaag ggcgtcggta actcgatccc ggatatcccc gatccggttg
    14341 ctcgtcatac caccgatggc ttactgaaac tgaaagtgaa aacgaactcc agctccagcg
    14401 tgttggtagg acctttattt ggttcttccg ctccagctcc ggttacggta ggtaacacgg
    14461 cggcaccagc tgtggctgca cccgctccgg caccggtgaa gaaaagcgag ccgatgctca
    14521 acgacacgga aagttatttt aataccgcga tcaaaaacgc tgtcgcgaaa ggtgatgttg
    14581 ataaggcgtt aaaactgctt gatgaagctg aacgcctggg atcgacatct gcccgttcca
    14641 cctttatcag cagtgtaaaa ggcaaggggt aattacgccc cacagtgctg attttgcaac
    14701 aactggtgcg tctcctggcg cacctttttt tatgcttcct tcctgggata tgagcgattt
    14761 tttatagtaa ctcacttctt cttcactaag aatatccatt atctcaatgc cttatcagag
    14821 attcttttcc tttcgccggt agtgtctgga cattcaggct acttttccag gttattttat
    14881 ttctgttatg cagaggtttt atgataagtc atatcctaaa ttctggcggc aataactctt
    14941 tgatgaaaca tgatgtggtg caaggaaata atatagtaga tcttgattta ctacgtaatt
    15001 tcaatggggt gccaggttta aacagagata actttattta tatcagcaat attttttcaa
    15061 atataaaaca acggaacgaa aaaatcatgc aataaatttg tttcgtgaag tctcaatcag
    15121 taatgatact ataagtgtaa aattctacag aaatgaaaaa aattgaatgc gcttgcaat
//
LOCUS       DAZGOB010000123        15083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGOB010000123 DAZGOB010000000
VERSION     DAZGOB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.123"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            273..929
                     /gene="pphA"
                     /locus_tag="ACHLIF_003963"
     CDS             273..929
                     /gene="pphA"
                     /locus_tag="ACHLIF_003963"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO9607879.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(930..1121)
                     /gene="yebW"
                     /locus_tag="ACHLIF_003964"
     CDS             complement(930..1121)
                     /gene="yebW"
                     /locus_tag="ACHLIF_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO9607880.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1226..1462)
                     /gene="yebV"
                     /locus_tag="ACHLIF_003965"
     CDS             complement(1226..1462)
                     /gene="yebV"
                     /locus_tag="ACHLIF_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO9607881.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1580..3019)
                     /gene="rsmF"
                     /locus_tag="ACHLIF_003966"
     CDS             complement(1580..3019)
                     /gene="rsmF"
                     /locus_tag="ACHLIF_003966"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO9607882.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3099..5732)
                     /gene="yebT"
                     /locus_tag="ACHLIF_003967"
     CDS             complement(3099..5732)
                     /gene="yebT"
                     /locus_tag="ACHLIF_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO9607883.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5701..6984)
                     /gene="yebS"
                     /locus_tag="ACHLIF_003968"
     CDS             complement(5701..6984)
                     /gene="yebS"
                     /locus_tag="ACHLIF_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO9607884.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7114..7611
                     /gene="msrC"
                     /locus_tag="ACHLIF_003969"
     CDS             7114..7611
                     /gene="msrC"
                     /locus_tag="ACHLIF_003969"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO9607885.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7708..8406
                     /gene="proQ"
                     /locus_tag="ACHLIF_003970"
     CDS             7708..8406
                     /gene="proQ"
                     /locus_tag="ACHLIF_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO9607886.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8426..10474
                     /gene="prc"
                     /locus_tag="ACHLIF_003971"
     CDS             8426..10474
                     /gene="prc"
                     /locus_tag="ACHLIF_003971"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO9607887.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPDPVK
                     "
     gene            10666..11547
                     /gene="htpX"
                     /locus_tag="ACHLIF_003972"
     CDS             10666..11547
                     /gene="htpX"
                     /locus_tag="ACHLIF_003972"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO9607888.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11593..12966)
                     /gene="yebQ"
                     /locus_tag="ACHLIF_003973"
     CDS             complement(11593..12966)
                     /gene="yebQ"
                     /locus_tag="ACHLIF_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9607889.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPARSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLKPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            13143..13934
                     /gene="kdgR"
                     /locus_tag="ACHLIF_003974"
     CDS             13143..13934
                     /gene="kdgR"
                     /locus_tag="ACHLIF_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO9607890.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14077..14316)
                     /gene="yobH"
                     /locus_tag="ACHLIF_003975"
     CDS             complement(14077..14316)
                     /gene="yobH"
                     /locus_tag="ACHLIF_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO9607891.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14475..14618
                     /gene="mgrB"
                     /locus_tag="ACHLIF_003976"
     CDS             14475..14618
                     /gene="mgrB"
                     /locus_tag="ACHLIF_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO9607892.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14693..14980
                     /gene="yebO"
                     /locus_tag="ACHLIF_003977"
     CDS             14693..14980
                     /gene="yebO"
                     /locus_tag="ACHLIF_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO9607893.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
BASE COUNT         3959 a         3660 c         3864 g         3600 t
ORIGIN      
        1 tggtaactaa ttgaatgtat taatatgtaa tagtacgtgt tagagattaa aagatgagca
       61 taaatttatt taacacatta attctaaaag attttgttgt ttgttgacga aaacaggaat
      121 cgtgttcggt ctctttttat ctgttaaaag ccagaagcat ttccttcgct gactttatag
      181 tcaaccataa cacacactct actgtctgag tccagcgttt tttaacattc ttgttaagat
      241 tatgtgatct ttagcgcggg aggaaaatat tgatgaaaca gcctgcgccc gtttatcaga
      301 gaattgcggg tcatcaatgg cgacatatct ggctttctgg cgatatacac ggttgtcttg
      361 agcagttgcg ccgcaaatta tggcattgtc gttttgatcc gtggcgagat ttacttatct
      421 cagtaggaga cgttatcgat cgtgggccgc aaagtttacg ttgtctgcag ttactggaac
      481 aacattgggt ttgtgcggta agaggcaatc atgaacagat ggcgatggat gcgctggcat
      541 cccagcagat gtctttgtgg ttgatgaatg gcggcgactg gtttattgcg ctggcagata
      601 atcaacagaa acaagcgaaa acggcgctgg aaaaatgtca gcatttgccc tttattcttg
      661 aagtacacag tcgtaccggc aaacatgtta ttgctcatgc cgattatcca gatgatgttt
      721 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
      781 aacgccaaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      841 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      901 gcgaactgac tcttgtgcaa ttgcaataat taaaaatcac catactcctg tgctggtcgc
      961 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg
     1021 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
     1081 ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
     1141 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
     1201 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
     1261 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
     1321 tttcagcatc ccaggcatcg agttgcatac ataaatcggg gtcagattta caaggaatgg
     1381 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
     1441 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgccgatt gtggtacgac
     1501 cagtccgatc gcaccatttt tcagcctagt tgaggcagcc gaatgtgcca gtcaaaaagt
     1561 gcgctttttt tgtgagctgt caggcgttac cggtaaaaag tttcccatcg cgcaccagtt
     1621 cacgcggata gctgtttttc aatcgcgaac caatccgttt ggctaaacca atcggttgat
     1681 gctggaaagt aaccaataca tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1741 cgcgatacca ctcctccgct tcctgcggtg tcagctcgaa tgcattcata ttatcgggtg
     1801 aggcaagagc aataaccgct tcatgctgcc agcgataacc tttgttgtgc gtctcggcaa
     1861 gtttaatccc caaccgagaa aatcggactt taccgatcag ggcttcaatg cccaccggga
     1921 acaaccacag ttctttgtca cgctgccaga ggcgcaggtt ttcatcccag tttaagccaa
     1981 cacctgcagt cgcctgacga atttgtccag cttcgcgatc tttcaccggg ctgaacggaa
     2041 aattaccgac tttgtatttg ggggcgggta aggcgggaat cgcctgagtt ttacgcagac
     2101 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aagccttctt
     2161 cggtcagcgc tttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctactgcgt
     2221 cggggtaagt ctctttcagc cacaggcaaa cggcttcgtt ttcttcctgg tttaaggtac
     2281 aggtcgagta aaccagcgta ccaccaggac gtaatgcatg aaaggcgctg tcgataagct
     2341 cccgttgtgt agctgcgatt tcctgattgc tttctggtga ccagtttttt agcgcatcgg
     2401 gatctttacg caccacgcct tcgccagagc agggagcgtc cagcaaaatg gcatcgaaca
     2461 tttctggcac tgccgcacca aacacgcggc catcaaaatg tgtgagcgca acattactga
     2521 tgccacagcg gctgatattg gcatgtaaca cttttacccg actggcggaa aactcattgg
     2581 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttg gagcctggcg
     2641 cagctgcgac atccatcacc cgctgtggtg cattatcgtc agcaaacaag gcggcaacgg
     2701 gcaacattga actggcttcc tgaatataaa acaggccgct taaatgctcg gcggtactac
     2761 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2821 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2881 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2941 caaatgagag cgtcgaaggc atcgcttcgc gcatttgtgt cagaaaggcg tccgggaaat
     3001 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     3061 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     3121 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcctgggct
     3181 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     3241 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     3301 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     3361 gcaatcatca cgcgatctga caatgtcccc agcgtcattc ctgtaaccgt accgacttcc
     3421 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg cgcttcaaca
     3481 ataatgctta agccatccag gtaacgcgaa tcagtaatgg tggcctcttg taattcaaag
     3541 tcgcggcgag gattgccccg gcctggttcg acgttgatat acggctggag gatagtatct
     3601 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     3661 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     3721 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3781 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     3841 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     3901 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     3961 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     4021 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     4081 ttagctcgcg gacggacggt aataacttca ccaacttcaa atttacggta gagcactacc
     4141 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     4201 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     4261 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     4321 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     4381 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     4441 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     4501 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     4561 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     4621 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     4681 ctaagggata atttcggatt acgtaattcg atgcgggtat tttcacgaag cagggtaacg
     4741 acgctgggat caactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     4801 gttagctgtc cgacttccag cccctgatac attaacggcg tcgagtcggc ggtcaatccg
     4861 gccccactcg gcagttccag ttttattatt acgccacgct ggctgtgggc cagatcttca
     4921 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     4981 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgacactg
     5041 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac cagatcggta
     5101 aaacgccgct cgatcagaac atcaatcacc acgccttgct tgttgggatt gatggcatag
     5161 tcgtagactt ttcccaccgg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     5221 ccgagatcgg gagcttgcag gtggatcatc agatcgccgt tatccagccg atacttcggt
     5281 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacccggcat catgccgata
     5341 tagttcccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     5401 accagccaga attgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     5461 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     5521 acgccctgat aacgaacagg cgtacggccc ggaacaatac catccgccga cataaagtcg
     5581 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     5641 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     5701 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     5761 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     5821 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     5881 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     5941 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     6001 tttagtaaac ggtaccagaa tactggcgat aaacacgatt cccgcaaccg caatgttgct
     6061 acttgccagc gacataattc cggaaagaat tgtatcttcc tgccgtcctc cgttcagata
     6121 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     6181 cgcccagcat ttttgcagac tatgacggcg acgcaggcgt agcgggatat ggcaacgcgg
     6241 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt
     6301 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     6361 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     6421 accagcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     6481 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     6541 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     6601 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     6661 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     6721 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     6781 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     6841 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     6901 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     6961 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatatt
     7021 aacccatgat tggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     7081 cagttccagg taagacaacc aagtgattat ataatgaaca aaacagaatt ttacgcggat
     7141 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     7201 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     7261 cttgaggacg atacactggt actaggacca tttcagggca aaattgcctg tgtccggata
     7321 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     7381 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     7441 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     7501 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     7561 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     7621 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     7681 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     7741 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     7801 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     7861 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     7921 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     7981 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     8041 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     8101 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     8161 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     8221 cctcgcgaag aacagcacac cccagtttct gacatttcag ctctgactgt cggacaagcc
     8281 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagac
     8341 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     8401 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     8461 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     8521 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     8581 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     8641 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     8701 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     8761 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     8821 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     8881 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     8941 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     9001 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     9061 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     9121 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     9181 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     9241 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     9301 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     9361 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     9421 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     9481 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     9541 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     9601 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     9661 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     9721 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     9781 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     9841 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     9901 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     9961 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
    10021 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
    10081 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
    10141 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatta tgaaggatat
    10201 cgcgcgcttc aacgctatga aggacaagcg caatatcgtt tctctgaact acgctgtgcg
    10261 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
    10321 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
    10381 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
    10441 cagacccgcg gaacaacccg atcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
    10501 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
    10561 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
    10621 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
    10681 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
    10741 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
    10801 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
    10861 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
    10921 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
    10981 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
    11041 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag tcacatcgcc
    11101 aatggtgata tggttaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
    11161 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
    11221 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
    11281 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
    11341 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    11401 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    11461 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    11521 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaggcg cgtcctgatc
    11581 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tcacacaggc
    11641 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    11701 ctgatttagc atcagcgcca ccagcgccgc gccactactc tgacccagta gacgagccgt
    11761 tcctaacatg ccactggccc caccgctacg ttcgcgcgga gcggaggtaa taatggtgtg
    11821 gttattgggt gactggaata agccaaatcc agcgccacat aagatcatcg gccagataat
    11881 attgatatcc gcaggtgacg cgggcagcag aaccagggaa aaaagccccg cagccatgat
    11941 gaacaaccct aaagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccag
    12001 cggagccatc accatcgttg ctaacggcca cggtttcaga agtaaacctg tttcgacttc
    12061 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    12121 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    12181 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    12241 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    12301 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    12361 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    12421 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    12481 tgctgcagca attgtcggcc cggcagcaga agaaacggca acaataaacg agtttatgcc
    12541 catccctctc cccagaaaac gttgtggata gatcaggcgg ataagtgcgg tattaacgct
    12601 catcaacgct gcaccgccga aaccttgtat gacacgcgca agggtgagca tttgcagcga
    12661 atcagaaagg gcgcagaaca gtgaagacaa cagaaaaacg accagaccgc atttataaat
    12721 acgtcgatag ccaaacatat cgcccagaaa cgaaaacgag agcagggaga tgacaatggc
    12781 gatttgatag gcgttcacta cccagatgga tctggctggc gtggcatgaa ggtccgtggc
    12841 gattgttggc agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat
    12901 cacaatggtt aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt
    12961 tggcatagga attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat
    13021 attcacggtg gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct
    13081 ggtgcgaaat tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat
    13141 aaatggctaa cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt
    13201 ttggcatttt gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc
    13261 gcgtcatgat gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt
    13321 atgtggcgca ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg
    13381 gcgctcgcgc gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga
    13441 tctcccgcct gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt
    13501 acattcacaa aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta
    13561 atccgctgta cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag
    13621 tgaagcaaat tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta
    13681 cagaagcgtt attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg
    13741 aagagcagga agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg
    13801 tcattgccgg tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg
    13861 aatatgtcgc aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg
    13921 actatccgtt ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt
    13981 tagcatgaga aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat
    14041 tttactcatg agtgagaatc tggttattta ttacccttaa ccattatcga ccacgatatt
    14101 gcttttacgt aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta
    14161 ttgtaccgtg ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc
    14221 attctctttg cttccaatca aaacgccata tccgctgagt aataatccta tccataccag
    14281 tgctatcagc ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt
    14341 cgcttaatcg tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat
    14401 atcgacatag ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg
    14461 aagcggagat tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt
    14521 gcttgctgct ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca
    14581 gcggaatttg tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat
    14641 tttcttcctc tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga
    14701 agttgtaaat tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct
    14761 tatcgggctc atcttgtggt tttttatcaa tcgtgccagt tctcggacta acgaacagat
    14821 tgaactgctt gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt
    14881 gtgcgaagca aacgaacctg agaaagcaga taaaaagacc gttgagagtc aaaaatcggt
    14941 tgaagacgaa gatattattc gtctggtcgc cgaacgataa tccttcctca atcttgagta
    15001 accccgtcgt tgaagggtta ctcaattcat cctgattata cgcactgcca tggtgcaact
    15061 gcttcaaatt aaaactctgg tct
//
LOCUS       DAZGOB010000124        15075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGOB010000124 DAZGOB010000000
VERSION     DAZGOB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.124"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(71..146)
                     /locus_tag="ACHLIF_003978"
     tRNA            complement(71..146)
                     /locus_tag="ACHLIF_003978"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Phe,seq:gaa)
     gene            complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHLIF_003979"
     CDS             complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHLIF_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO9607894.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHLIF_003980"
     CDS             1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHLIF_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO9607895.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            1357..3492
                     /gene="speC"
                     /locus_tag="ACHLIF_003981"
     CDS             1357..3492
                     /gene="speC"
                     /locus_tag="ACHLIF_003981"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO9607896.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHLIF_003982"
     CDS             complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHLIF_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO9607897.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHLIF_003983"
     CDS             complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHLIF_003983"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO9607898.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHLIF_003984"
     CDS             complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHLIF_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO9607899.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHLIF_003985"
     CDS             complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHLIF_003985"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO9607900.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7660..8379
                     /gene="trmB"
                     /locus_tag="ACHLIF_003986"
     CDS             7660..8379
                     /gene="trmB"
                     /locus_tag="ACHLIF_003986"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO9607901.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8379..8705
                     /gene="yggL"
                     /locus_tag="ACHLIF_003987"
     CDS             8379..8705
                     /gene="yggL"
                     /locus_tag="ACHLIF_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO9607902.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8889..9608
                     /gene="yggN"
                     /locus_tag="ACHLIF_003988"
     CDS             8889..9608
                     /gene="yggN"
                     /locus_tag="ACHLIF_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO9607903.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9784..10830
                     /gene="ansB"
                     /locus_tag="ACHLIF_003989"
     CDS             9784..10830
                     /gene="ansB"
                     /locus_tag="ACHLIF_003989"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO9607904.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVAAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10947..11954
                     /gene="yggM"
                     /locus_tag="ACHLIF_003990"
     CDS             10947..11954
                     /gene="yggM"
                     /locus_tag="ACHLIF_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO9607905.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQAFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(12010..13311)
                     /locus_tag="ACHLIF_003991"
     CDS             complement(12010..13311)
                     /locus_tag="ACHLIF_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO9607906.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(13311..13814)
                     /locus_tag="ACHLIF_003992"
     CDS             complement(13311..13814)
                     /locus_tag="ACHLIF_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO9607907.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(13859..14845)
                     /locus_tag="ACHLIF_003993"
     CDS             complement(13859..14845)
                     /locus_tag="ACHLIF_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO9607908.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         4011 a         3598 c         3796 g         3670 t
ORIGIN      
        1 ggcaggacgc ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt
       61 tatatgaatt tggtgcccgg actcggaatc gaaccaagga cacggggatt ttcaatcccc
      121 tgctctaccg actgagctat ccgggcaacg gggcgcatta aacctgattc gcctcgtctc
      181 gtcaatcaaa tttcttcaat ttactgcaga ctgcacaatc tatcatcact ttgccgctat
      241 tgcacgcatt gtcaggcaaa ccaggcggtc caggcggcgg aaagcggcat tgccagaagg
      301 agtgctggaa gcatgttgac caccgggaac attttaatgc cacagatgcg taatccggtt
      361 gccagcagca ataaaccgcc tacggcgctg aagtctgcca tcatcgacgg tgtggtcagc
      421 ggtaatatca gcgcggcagc ccacgccagc gttaactgga tgatcagtaa tgggatacta
      481 attaccgaca ccgcaatacc cagcgagcag gcgaagatca tcgccgtaaa gaaatcaaga
      541 aatgacttgg cgattaaaat actcggatcg ccggtcatcc cttcgttcat cgccccgaag
      601 atcccggtgc cgctggcgca aaacaggaca ataatcgcga cataattctg aataaaagat
      661 tcatgcgctg gcttcttacg tgagtgacga aacagatttt gtgctttggc gactgctgta
      721 ttgacacctt tctccagcaa acaaatttcg ccaattagag cgccaagtag cgtagctaaa
      781 accatcgctg gaaggttggc gcatttcaca accagtaaaa taccaatccc cagcgatgcc
      841 agaccaaaaa tagatgtcat ggagacgcgg atacgttccg gtaagcgttg gctgagcagt
      901 gcgccgagaa cgccacccag taagactgca ctagcgttga taaaagggcc gatgaccaca
      961 agagttcctg ttagctgtat tgtttgattt tcttattatg aactttttgc cttgttgatg
     1021 gcttgtctgg tcgcaaagtc ctggcttgca cgctttagcg aaaggtgcca tgattgcgcg
     1081 aattttctcc tctctgtacg gagtttgccc gatgcacgcc atctccttac attctctcgc
     1141 ttatcgccgt ttcgcgcgaa acgtttccct ttctatgtta ctgctcatgc ggtgaagtta
     1201 acgcacgctc actgcaggac aacagtaaaa tcagagcgtt tctgctttta ctgatgtctg
     1261 gcggtcggag ctggtgacca gtttgaccca tatctcatgg ggcagggttt tccaccttgt
     1321 ccggtattct tacttccccg aaacgggttt gcgcttatga aatcaatgaa tattgccgcc
     1381 agtagtgaac tggtatcccg actttcttct catcgtcgcg tggtggcgtt gggagatact
     1441 gattttacgg acgtcgcggc agtcgtcatt accgctgcgg atagtcgcag tggcattctt
     1501 gcgttgctta agcgcaccgg ttttcatcta ccggtgtttt tgtattccga acatgctgtt
     1561 gaattacctg cgggcgttac ggcggtaatc aacggcaacg agcagcagtg gctggagctg
     1621 gaatccgcag cctgtcagta tgaagagaat ttgctgccac cgttttatga cacgctgacg
     1681 cagtacgttg agatgggcaa cagcaccttt gcttgccctg gacatcaaca tggtgcgttt
     1741 tttaaaaagc atcctgccgg acgccatttt tacgatttct ttggtgagaa cgtctttcgc
     1801 gccgatatgt gtaacgctga cgtaaaattg ggcgatctgc ttattcatga aggatcggcg
     1861 aaagatgcgc agaaattcgc agccaaagtc tttcatgccg ataaaaccta ttttgtgctg
     1921 aacggcacat cggcagcgaa taaagtggtg acgaatgcgc tgttaacgcg tggcgatctg
     1981 gtgctcttcg accgtaacaa ccataagtcg aatcatcacg gcgcgctgat tcaggcgggg
     2041 gcgacgccgg tctatctgga agcttcacgc aacccgtttg gtttcattgg cggtattgat
     2101 gcgcactgtt ttaatgaaga gtatctgcgc cagcaaattc gcgacgttgc gccagaaaaa
     2161 gccgagctgc cgcgcccgtt tcgcctggcg attattcagc tgggaaccta tgacggaact
     2221 gtctataacg cccgtcaggt gatcgatacc gttgggcatc tgtgtgatta cattctgttt
     2281 gattccgcgt gggtcggtta cgaacaattt atcccgatga tggcggatag ctcgccgctg
     2341 ctgttagaac ttaacgaaaa cgatccgggg atttttgtga cccagtcggt gcacaaacag
     2401 caggctggat tctcacagac gtcgcagatc cataaaaaag ataaccatat ccgcggacag
     2461 gcgcgttttt gcccgcataa gcggttgaat aacgccttta tgctccatgc ttctaccagc
     2521 cctttctatc cgctgtttgc tgcactggat gttaacgcca aaattcatga aggggagagt
     2581 gggcgtcggc tgtgggctga gtgcgttgag ttgggtattg agtcgcgcaa ggctattctt
     2641 gcgcgctgta agctgttccg cccgtttatc ccgcccgttg ttgatggcaa attgtggcag
     2701 gattatccga catctgtgtt agccagcgac cgccgttttt tcagttttga gccgggggcg
     2761 aagtggcacg gctttgaagg atatgccgcg gatcagtatt ttgttgatcc gtgcaagctg
     2821 ttactcacta caccaggtat caatgccgaa actggcgaat atagcgactt tggcgttccg
     2881 gcgacgattc tggcgcacta tctgcgtgag aacggcattg tgccggagaa gtgcgatctc
     2941 aactccattc tgttcttatt aactccggcg gaaagccacg agaagctggc acaactggtg
     3001 gcgatgctgg cgcaatttga acagcatatt gaggatgact cgccgctggc tgaggtgttg
     3061 ccgagcgttt ataacaaata tccggtgcgc tatcgcgact acaccctgcg ccagttgtgt
     3121 caggagatgc acgatctgta tgtcagtttc gacgtcaaag acctacaaaa agcgatgttc
     3181 cgccagcaga gtttcccgtc agtggtgatg aacccccagg atgcgcatag cgcttatatt
     3241 cgcggtgaag tggagttggt gcggattcgt gatgccgaag ggcgaattgc ggcagaaggg
     3301 gcgttgcctt atccacctgg cgtgctttgc gtggtacccg gggaagtctg gggtggggcg
     3361 gttcaacgtt atttccttgc actggaagaa ggggtgaatt tgttgccggg attttcgccg
     3421 gagctgcaag gtgtttatag cgaaaccgat gcggatggca tgaaacggtt gtacggttat
     3481 gtgttgaagt aagaataaaa aaaacgggtc accttctggc gacccgtttt tctttgcgta
     3541 attagtggct aaccgtctgt gtgcctgtcg ggacacgaac gtgtttatat ttgaacatcg
     3601 ccatgaacgc gaaggccaga accacggagt aaccagcgaa aatcaaccat acggtctgcc
     3661 agtcggtaat gccgttttgg gtgtacatct caacaacttt accgctcacg atgccgccga
     3721 ggatacagcc gaagccgtta gtcatcatca ggaacatccc ttgtgcactg gcgcgaattg
     3781 ccgggctaac ttctttttcg acaaacaccg aaccagagat gttgaagaag tcgaatgcgc
     3841 aaccgtaaac gatcatcgac agtaccagca gtacagtacc gaacggagtc gggtcgccgt
     3901 aagcaaacag cgcaaaacgc aggatccacg ccacaatact gatcatcatt acgttcttaa
     3961 taccgtagcg gcttaagaag aacgggatgg tcagaatgaa cagggtttca gagatctgcg
     4021 aaatcgacat gatgattgac gcatgctgca caataaagct gctggcaaac atcggatctt
     4081 tgtcgaagct gtgcaggaag gtattaccga acatattggt aatctgcagt tccgcgccca
     4141 gcagcattga gaagataaag aagattgcca tacgcttgtt tttaaacagc gcgaatgcat
     4201 cgaggcccag cagggttgtc cagctctgat tcgcttgctg tttagcaacc ggaatatgcg
     4261 gcagagtcag ggtaaacaga accagaatgg cggaaagtgc tgcgccaata tacagctgca
     4321 tgtggcttaa ttcgaagcca gacaggctca ccacccacat tgccatgata aagccgatgg
     4381 tgccccagat acggattggc gggaagtcag taacgatatc catcccggca ttttgcaggc
     4441 gatagtaaga gatggtgttg attaacccaa gcgttggcat ataagcaaac gagttaatca
     4501 atatcacaag gaacatcgcc tccggtgtcg tgacctgtgc cgccatgaac agcgtgatag
     4561 cgccaatggt gtggcaaatg gcatataccc atttcgcact taaccatttg tcggccacaa
     4621 tccccagcag cgcaggcata aagaccgcgg cgatacccag tgagctataa actgcgccaa
     4681 tagaagcacc gtcaaacttc agggtaacaa acatatagga gccgagggtc gtcagccaac
     4741 ttccccacag acagaactgc agaaaagaga ggattttcag ctgcagctta agattcatgt
     4801 taatttcctc acatcgtgat gcggatgagt gtttgaaaaa gcacatttga ccgtgcgaga
     4861 tgatgggatt ctatcaactt cgcggactgg ttttttgtta cctgtggcaa ataattgcaa
     4921 acattcccaa tttgcaaaac gaaattgtga tgtggataac atttttgccc ctgagcatcg
     4981 tcaggggcgg ttaatggaat tatcggcggc ggtaggattt ttgcgcggta ttcactttat
     5041 aaagataacg gcgagattcc gcagagggat ggcgggtcgt cagcgtctga taaacatcgc
     5101 ccggcgtcat ggtgttaata atattggcag cctgaatctt atcattcgaa aagactcgca
     5161 gcacgctgcc tgcgccgccg ttataggcgg tgatgacggc ataacgccgc gatgttgggt
     5221 tatcaattcc gccgagataa acattgttca gcatcgccag atacgcggtg ccggtatcaa
     5281 tattgctggc aggatcaaac aagaaactgc ggctcggcgt gccggatttc ccctgcgagc
     5341 ggaacacatc tttcccggca gtatgttgta ccacctgcat taatcccagc gcatcggaac
     5401 ggctgaccgc atatgggtta aaggaagatt cggtctgcat aatcgccaga atcagcgact
     5461 catcaacgcc atatttccgt gacgcctggc ggaccatgcc gagatatttg tgcgcacgtt
     5521 tatcaaggtg gttcggcacc atgttaatgg taacgctgta gatgatacgc agcccgttgc
     5581 tgcggctctt cagacggttt ttcagcagat aatccgcgaa gttgctcgcg cgaccttccc
     5641 agcgaatcgg ctgcccggtg ttgtccacca cctgaccgta aaggaaaggt tctttcgaaa
     5701 tcgtaatatc atcaacgtcg gaatagagat cgaccgaact cggatcgtca cccatcagca
     5761 acgttttgat aattgcccgg cgcaaatgcg cggcaggttc tgtcccggcg atagtttcga
     5821 tagtaatcgt accgtcatcg aagttgatgt ggctgcgggt ctgatattga tcggtgtatt
     5881 tcacgtagtc cttaggaccg gcgatcacca cctctttgaa tccccagatg ttctcaatat
     5941 tgtgggcaaa ttgccccatc agaatatcaa aaccgttggt atctttgacc caggcttcgt
     6001 tataggtatc gccttttttg gtcgtcgaac aggagatgag caacggcgca atcaaagcca
     6061 gcgcgagata ttttttcatc attccgggtg cgtgttgtgt ttgtttttat gcaagttgcc
     6121 ggaggcgtgc tccggcactg tttttatttt ttatcttccg gcgtatagcc ctcgatatgc
     6181 acctctttac cctcgaacag gaagttgacc atctcctgct ccagcagctt gcggtgctcg
     6241 gcattcatca tgttgagttt cttttcatta atcagcatgg tttgtttgtg ctgccactgc
     6301 gcccaggctt ctttggagat ctcgttatag atgcgttttc ccagctcgcc ggggtacagc
     6361 tgaaaatcct gaccttctgc ttcacgttgc aggaaagtac aaaaaatcgt tctgctcata
     6421 aatcatcctc tttatcgact cacgcgctaa accggcgcgc cagtgcgtaa ctgctgtaac
     6481 aaacgctcca cgggagccgc caggccaact gacggcggtt gcgctaagtt ataccagagc
     6541 gcattgcctt catccatgca gccggtgaat gacgacacgg gaagccacat aggcacaata
     6601 tctaagtgga aatggctgaa ggtatgccga aacgcggtca gctgcgtcag gttatcggca
     6661 gcaatctgcc gttgcgccag ccactgccgc aaactttctt cgtcggcaaa ctgcgggaaa
     6721 cagtataaac cgccccacaa tccgctcggc ggacgctgcg ccagcaatac ttcatcttcg
     6781 tgctgtaata gcaaaaagta gccggtgcgc tccggcagcg tctgtttcgg ttttttgccc
     6841 ggataaagcg cccagctatt gttggcggcg gcaatacatc cgttttgtag cggacagagc
     6901 gaacatttcg gtttcgagcg cgtacaaatc atcgcaccca aatccatcat cgcctgatta
     6961 aaccgttcca cgccaaccgc gggcgtcacc tgctcgctca aactccataa tttattctcg
     7021 acctcttttt tccctggcca gccgcttaca gcatagcagc gcgccagcac ccgtttgacg
     7081 ttaccgtcga gaatcggaaa gtgcttaccc agagaaagcg agagaatcgc acctgcggtg
     7141 gaacgcccga cgcccggtaa cgccgcgact tcttcaaagg tttccgggaa tttaccgccg
     7201 tgtaaggtcg ccacttgttg tgccgcttta tgcaggttgc gcgcgcgggc gtaatagcca
     7261 agcccggtcc acaagtggag aacttcgtcg agcggcgcat tggcgagatc ggtcaccgtc
     7321 gggaagcgcg ccataaagcg ttcaaaatag gggataacgg tcgcaacctg agtttgttgc
     7381 aacatcactt ctgagagcca tactttgtag ggcgtcttgt caatttgcca gggcagcgtt
     7441 ttccgcccgt atttatcgta ccagtccaga acctgggctg aaaattgcga cgcttgcatg
     7501 gtcaccgaat tcactgttgt tgggggcaag attgcagcac agcgacggca gggtgtaaac
     7561 cggaactttc cgcagccacg gccttatcat gcttgcatcc ggcaactaac tttggataat
     7621 gcccgttttc agaacacttt cacaagcgac taaatcttta tgaaaaacga cgtcatttca
     7681 ccggaatttg atgaaaacgg ccgcccactg cgccgtatcc gtagttttgt gcgccgccag
     7741 gggcgactga ccaaaggcca ggaacatgcg ctggaaaact actggccggt gatgggcgtt
     7801 gagttcagcg aagatatgct ggatttcccc gcgctttttg gccgtgaagc gccggtgacg
     7861 cttgagattg gttttggcat gggggcgtcg ctggtggcaa tggctaaaga tcgccctgag
     7921 caggacttcc tcggcattga agtgcattca ccgggcgttg gtgcgtgcct ggcttctgcg
     7981 catgaagaag gtttaagcaa cctgcgcgtg atgtgtcacg atgcggttga agtgctgcat
     8041 aaaatgattc ctgacaattc attgcgcatg gtgcagctct ttttccctga cccgtggcac
     8101 aaagcgcgcc ataataaacg ccgtatcgta caggtgccgt ttgccgaact ggtaaaaagc
     8161 aaactgcagc tggggggcgt attccatatg gcgaccgact gggaacctta tgcggaacat
     8221 atgcttgaag tgatgtcttc tattgacggt tataaaaacc tgtcagagag caatgattac
     8281 gtaccgcgtc cggcatcacg tccggtgacg aaatttgaac aacgtggtca tcgtcttggt
     8341 cacggagtat gggacttaat gttcgagagg gtgaaataat ggcaaagaac cgtagccgtc
     8401 gtctgcgtaa aaaaatgcac atcgacgaat tccaggaatt aggattttcg gtggcatggc
     8461 gattcccgga aggtacatcg gaagaacaga ttgataaaac cgttgatgat tttattaacg
     8521 aggttatcga accgaacaaa ctggcctttg acggcagcgg ttatctggcc tgggaaggtc
     8581 tgatctgcat gcaggaaatc ggcaaatgca ccgaagaaca tcaggcgatt gtgcgtaagt
     8641 ggctggaaga gcgcaaactg gatgaggtac gcaccagcga acttttcgac gtttggtggg
     8701 actaagaaag catacgggcg atgacaaatg caaaactgcc tgatgcgcta cgcttatcag
     8761 gcctggaaag atgcacgatc gagtaggcgg gataaggtgt ttacgccgca tccggcatgg
     8821 aaaacgcgta ctttgttatc aatctggggc cagcaaatgc tggcctgatt ttttcttgag
     8881 ggaagactat gatgcgcaaa atgctgctgg cggcagcact ttcagtgacg gcaatgaccg
     8941 ctcacgccga ctaccagtgc agcgtcacgc cgcgtgacga tgtgattgtc agcccgcaaa
     9001 ccgtgcaggt gaagggcgaa aacggcaatc tggtgatcac gccagacggc aacgtgatgt
     9061 ataacggtaa gcaatattcc ctgaatgccg cccagcgcga gcaggcgaag gattatcagg
     9121 ctgaactacg cagcacgctg ccgtggattg atgaaggcgc gaaaagccgc gtcgaaaaag
     9181 cccgtattgc gctggataaa attatcgttc aggagatggg cgaaagcagc aaaatgcgca
     9241 gccgtctgac caaacttgat gcgcagctga aagagcagat gaaccgcatt atcgaaacgc
     9301 gcagcgatgg cctgacgttt cactataaag ccattgatca ggttcgcgcc gaaggccagc
     9361 aattagtgaa tcaggcaatg ggtggaattt tacaggacag cattaatgaa atgggcgcga
     9421 aagcggtgct gaaaagcggc ggtaacccat tacagaatgt gctgggaagc ctgggggggc
     9481 tgcaatcctc aatccaaacc gagtggaaaa agcaggaaaa agatttccag cagtttggca
     9541 aagatgtttg tagccgcgtt gtgactctgg aagatagccg caaagccctg gtcgggaatt
     9601 taaaataatc ctctatttta agacggcata atactttttt atgccgttta attcttcgtt
     9661 ttgttacctg cctctaactt tgtagatctc caaaatatat tcacgttgta aattgtttaa
     9721 cgtcaaattt cccatacaga gctaagggat aatgcgtagc gttcacgtaa ctggaggaat
     9781 gaaatggagt ttttcaaaaa gacggcactt gccgcactgg ttatgggttt tagtggtgca
     9841 gcattggcat tacccaatat caccatttta gcaaccggcg ggaccattgc cggtggtggt
     9901 gactccgcaa ccaaatctaa ctacacagcg ggtaaagttg gcgtagaaaa tctggttaat
     9961 gcggtgccgc aactaaaaga cattgcgaac gttaaaggcg agcaggtagt gaatatcggc
    10021 tcccaggaca tgaacgataa tgtctggctg acactggcga aaaaaattaa caccgactgc
    10081 gataaaaccg acggcttcgt cattacccac ggtaccgaca cgatggaaga aaccgcttac
    10141 ttcctcgacc tgacggtgaa atgcgacaaa ccggtggtga tggtcggcgc aatgcgcccg
    10201 tccacgtcca tgagcgcaga cggtccattc aacctgtata acgcggtagt ggccgcagct
    10261 gataaagcct ccgctaatcg tggcgtgctg gtggtgatga acgacaccgt actggacggt
    10321 cgcgatgtca ccaaaaccaa caccaccgac gtagcgacct tcaagtctgt taactacggt
    10381 cctctgggat acattcacaa cggtaagatt gactaccaac gtaccccggc acgtaagcac
    10441 accagcgata cgccattcga tgtctctaag ctgaatgagc tgccgaaagt cggcatcgtt
    10501 tataactacg ctaacgcatc cgatcttccg gctaaagcac tggtagatgc gggctatgat
    10561 ggcatcgtta gcgctggtgt gggtaatggt aacctgtata aatccgtgtt cgacaccctg
    10621 gcaaccgccg cgaaaaacgg cactgcagta gtgcgttctt cccgcgtacc gacgggtgct
    10681 accactcagg atgctgaagt ggatgatgcg aaatacggct tcgtcgcctc tggcacgctg
    10741 aacccgcaaa aagcgcgcgt cctgctgcag ctggctctga cgcaaaccaa agatccgcag
    10801 cagatccagc agatcttcaa tcagtactaa tcacctcgcc ccggtatcgt gccggggctt
    10861 tttcacttca gacttacgtc cattgccaat tttcattacc ctaatgataa tcaccggaat
    10921 aaattattcc gcgtgaggtt tttcgggtga aaaagcaatg gattgttggc acggcactgc
    10981 ttatgttgat gactggtaat gcctgggcag atggcgaacc gccaactgaa aatatcttaa
    11041 aagatcaatt caaaaagcag tatcacggca ttctcaagct tgatgctatc accttaaaaa
    11101 atcttgatgc taagggtaat caggccacct ggtcagcgga aggcgatgtc tcttccagtg
    11161 acgatctcta tacctgggtc ggtcagttgg cagattacga actgctcgaa cagacctgga
    11221 cgaaagataa accggtaaaa ttctcggcga tgttaaccag taaaggaacg cccgcgtctg
    11281 gctggtcggt gaacttttac tcttttcagg cggcagccag cgatcgtggg cgggtggtcg
    11341 acgatatcaa aacgaataat aaatatctga tcgtgaatag cgaagatttc aattatcgct
    11401 ttagtcagct tgaatctgcg ttgaataccc agaagaactc gattcctgcg ctggaaaagg
    11461 aagtgaaagc gcttgataag caaatggtgg cggcgcagaa agcagcggat gcatattggg
    11521 gaaaagatgc aaacggtaag caaatgaccc gcgaagaagc ctttaaaaaa attcaccaac
    11581 agcgtgatga gtttaataaa cagaacgata gcgaggcgtt cgcggttaaa tatgacaaag
    11641 aggtttatca accggcgatt gcggcatgtc ataaacagag tgaagagtgt tatgaagtgc
    11701 cgattcagca gaaacgagat ttcgatatca acgaacaacg gcgacaggct tttctgcaat
    11761 cacaaaaact cagccgtaaa ttgcaggatg actgggtaac gcttgaaaaa gggcaatatc
    11821 cgttaacgat gaaagtttca gaaataaaca gtaaaaaagt cgcgattttg atgaaaattg
    11881 acgatatcaa ccaagctaat gaacgctgga aaaaagatac cgaacaactg cggcgcaatg
    11941 gggtgattaa gtaagtttga tatagtgcct ttgccggata atataaaaag ttatccggca
    12001 aattattttt tacattagcc ctagtagctg tggaatgaac aaggatattt gcggaatgta
    12061 agtgatcatc atcaacgcta ctagcaatgc agcataaaat ggcagcaatg gtttgattaa
    12121 atgttgaatc ttcacacccg agatagaaca accaacaaat agcgcactac ccaccggcgg
    12181 tgttaacagc ccaatacaaa ggttggccac catcataata ccgaaatgta ctgggtccat
    12241 tcccagctct tgtgcaatcg gcaagaagat cggtgtaaag attagtacag ccggtgtcat
    12301 gtccataaag atgccgacaa ttaataagac gatattaatc agcagcaata tgatcagtgg
    12361 gttatcagaa atccccatca atgcgtcgct gatcatataa ggaatatcag cattagtcat
    12421 tgcccaggac atgcccacgg aaaaaccgat caacagtaat acaatcgaag tggtaacaac
    12481 cgactccaga atcagtttgg gtaaatggcg ccattttact tcccggtaaa ccagcaccga
    12541 gagaataaat gtgtagacaa cggcaattgc tgacgcttca gttgcggtaa aaatgccgcc
    12601 cagaatgccg cccatcacaa tgaacaccag aagcaaactt ggtaaggcat ccagaaacgc
    12661 tttacaggcc tgagcaaagg ttgctctttc tgaaagcggg tagccacggc gtttagcaat
    12721 aatgccgcag actatcatta ccgcaagacc cattaagatc cctggcaaat atcccgccat
    12781 aaatagcgat gctacagaaa cgccgccagc tgtcagggaa aacacaatta acacgtttga
    12841 gggcggtatc agtaatccag taatacaaga tgagacgttt acagcggtag aaaatgccgg
    12901 atcataacct tgtttggtct gaatgggatt cagtgtgcca ccaacggcag cagctgccgc
    12961 aacggctgaa ccagaaatgg aaccaaacat catattcgcc aggacgttca catgaccaag
    13021 tgaaccagga acccgaccaa ccattacctg ggcgaggtta atcaaacgta tcgcaatacc
    13081 gccgctgttc atcagcgttc cggcaaagat gaaaaagggg attgccaata gggcaaagtt
    13141 atccagtcca ttagccagtc gctgcgaaac agtgattgcc gcgatatccc agggaaacat
    13201 caaaagcatt gaagctacag tggcaatacc gattgagaac gaaatgggca caccaataaa
    13261 gaccaacaca aaaaaacagc caaacagtgt aatcgcgata taactatcca ttaactggcc
    13321 tcctgcgcat gttgtttcag ttgttgaatg ctttgattaa taaaacacaa ggcataaaac
    13381 atcatcacca atccacttaa tggcagaatg atatatacgt atcccatcgg tatttgcatc
    13441 gcggcagata cctgtgatgt tgccagcgtt ttatcaatta atttcaggcc accagtcaca
    13501 atgaccgaac cggcaaaacc taaaataaga acgttaatga ctatactaag aagtaattgc
    13561 ttgcgcttat tcagggcgag agcaaataaa tcgatagaga gatgtctctg cgcgccgacg
    13621 gtatatgcag cacctaataa ccctacccag atcatcaaaa atcgtgccag ctcatcggtt
    13681 aatgtacttg gctggttaag tacgtagcgg ctaaatactt gccagactac gcatacaacc
    13741 agagcaagca tgacggcgac agaaaacgcg gcaatgacgc ggtctaccgc aagtttcagg
    13801 cggttaaata tcattgtaag catcctggaa aatcagggcc agagactggc ccggaatatt
    13861 attgagcagc gttgtcgaac ttctctaaca aagcagcttg ttttggatct tttttgaagt
    13921 cgtcaaacag aggctgaact gcggcacgga atggtgcttt atccactttt acaatttcac
    13981 cccccatagc tttcgcctga gcacgtgtgt cagcgtcaat tttttcccac aatttttgtt
    14041 gataagcttc ggatttcttc gctgctgttt cgaggatttg ttgctgttct ggggttaatt
    14101 tattccatgt tttggtggag ataaccaaaa agtcaggaat tgaggcgtgt tcatcttcag
    14161 aaaaaacttt ggcaatttca atatgtcgag tctgcaccca ggaaggcacg ttattttctg
    14221 cgccatctac tacaccttgc tgcatcgcag tataaacttc gccaaatgaa attggagtgg
    14281 gtgaaccacc catcagttca atcattttga tggtggtcgg acttggctga acacgaattt
    14341 tcagcccttt aagatcttct ggcttagtaa tcggtttttt cgcatagaaa ctgcgtgtac
    14401 ccgctacata agctgataaa gcaaaaaagc ctttgtcttt tgttgagtcc ataatttctt
    14461 taccaacttc accaaaaaca actttgttga agtgagcctg atctttaaat aagaacggca
    14521 aattatagat agcataaata ttatcaaaag actccagatc actggcggaa cctttggtca
    14581 tatccagagc gccattttgc aacaactcca tagtttctcg cgcattaccc atctggctgc
    14641 tgggataaat acggatcacc atattacctt ttgacagctg ttttacctct ttcgccagct
    14701 cttcaaaact ctgatgtaca acatgacttc tttccaggtt gtgtgctaac tttaacgtca
    14761 ctttttctgc ggcgtttacg ccagtgctaa ccagagccag caaggatgcg cagactaatg
    14821 agcgggtaaa tgacctcatt ttcataaaaa aactccctgt tgaaggatgg aaattggttg
    14881 atatctaatt gtgcttatca gtgctttgtg cgacaaattg gttaacctgt ctgacaactt
    14941 gtctatgttt atcacaactt gaaataaagt cagaaattaa atattaaatt tgtgcgccag
    15001 gttgtacatg tttataatca ataaatgacg agtaaatatt cactatatat ataataaata
    15061 tcgtcgatat ttatt
//
LOCUS       DAZGOB010000125        14999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGOB010000125 DAZGOB010000000
VERSION     DAZGOB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.125"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..165
                     /locus_tag="ACHLIF_003994"
     CDS             <1..165
                     /locus_tag="ACHLIF_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO9607909.1"
                     /translation="GGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSEL
                     LPWRIALPAE"
     gene            complement(228..416)
                     /locus_tag="ACHLIF_003995"
                     /pseudo
     CDS             complement(228..416)
                     /locus_tag="ACHLIF_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(469..1467)
                     /gene="lpxM"
                     /locus_tag="ACHLIF_003996"
                     /gene_synonym="msbB"
     CDS             complement(469..1467)
                     /gene="lpxM"
                     /locus_tag="ACHLIF_003996"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO9607910.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLIAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            complement(1541..3262)
                     /gene="cptA"
                     /locus_tag="ACHLIF_003997"
     CDS             complement(1541..3262)
                     /gene="cptA"
                     /locus_tag="ACHLIF_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO9607911.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            complement(3356..4462)
                     /locus_tag="ACHLIF_003998"
     CDS             complement(3356..4462)
                     /locus_tag="ACHLIF_003998"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO9607912.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            complement(4462..5283)
                     /locus_tag="ACHLIF_003999"
     CDS             complement(4462..5283)
                     /locus_tag="ACHLIF_003999"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO9607913.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            complement(5884..6018)
                     /locus_tag="ACHLIF_004000"
     CDS             complement(5884..6018)
                     /locus_tag="ACHLIF_004000"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9607914.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            6756..7408
                     /locus_tag="ACHLIF_004001"
                     /pseudo
     CDS             6756..7408
                     /locus_tag="ACHLIF_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    7037..7153
                     /locus_tag="ACHLIF_004001"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            7931..8446
                     /gene="ehxC"
                     /locus_tag="ACHLIF_004002"
                     /gene_synonym="hlyC"
     CDS             7931..8446
                     /gene="ehxC"
                     /locus_tag="ACHLIF_004002"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO9607915.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            8448..11444
                     /gene="ehxA"
                     /locus_tag="ACHLIF_004003"
     CDS             8448..11444
                     /gene="ehxA"
                     /locus_tag="ACHLIF_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO9607916.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGRTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARMISELGDKDDVVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            11494..13614
                     /gene="ehxB"
                     /locus_tag="ACHLIF_004004"
                     /gene_synonym="hlyB"
     CDS             11494..13614
                     /gene="ehxB"
                     /locus_tag="ACHLIF_004004"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO9607917.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            13618..>14999
                     /gene="ehxD"
                     /locus_tag="ACHLIF_004005"
     CDS             13618..>14999
                     /gene="ehxD"
                     /locus_tag="ACHLIF_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO9607918.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSV"
BASE COUNT         4471 a         2991 c         3352 g         4185 t
ORIGIN      
        1 ggtggtgagc ggggagcgct actgtacagc ctgatcggga cgtgcaaact gaatgacgtg
       61 gatccagaaa gctaccttcg ccatgtgcct ggcgtcatag cagactggcc ggtcaaccgg
      121 gtcagcgaac tgcttccgtg gcgcatagca ctgccagctg aataacacat ccccgtcaat
      181 acggccctcg ctgtacgctt acgaccgctc agtaaatatc aatacagttc tgacgagccg
      241 cttacaagct gtgattcata cagactgagc acacgggctg cgacagcaac accgatgcgt
      301 tcagcaagct tcagggcttc actgcgaaat tcaggcgaat gctatttacg gggtttttta
      361 ctggttggta ctgttttttt gtcatatgag ccacctctga ctgagagttt actcacttag
      421 cgtgtgtcca ctattgctgg gtaagctcat tatcctgaaa aacctgcctc agtaaggatc
      481 ctcttcccct tctttacgcg ttttcagcaa cttgaggatc cacgtatact gctcaggatg
      541 tggacgaacg aaattttcaa ccacttcgtt tatctgtcgc gcaatatgag catcactctt
      601 ctgtcgtata gcaagtggcg ggctgacatg aatagtcaga tgatgggtct ttccgtcata
      661 aacagggaaa agcggtatta tccgtgcacc actgatcctg ctgagccgtc cgataaccgg
      721 taaagttgct ttatacgtcg caaaaaaatc tgcaaactca ctgaattccg ggccatgatc
      781 ctgatcagga agataatatc cccagtatcc ctgccggact gagcggacaa aggaggcaat
      841 accgtcatta cgtgcgtgca gttttcctcc gaaacgacga cgaacactgt tccagacata
      901 atccaccacc ggatttctct ggttatgaaa catcgctgcc atttttctgc ccgacgcagc
      961 tatcagcatt gcgggaatat ccactcccca ggcatgtggc acaagaaaaa tgaccttttc
     1021 attgttctgt gccatcttct ccacaatctc cagaccattc cagcgtatac gatgcgaaat
     1081 tttatcaggc ccggacagtg ctaattcagc cataagcaca acggccatgg aggctgttgc
     1141 aaacatggca tcaacgatat tctccttttc tttatcactg tactccggaa aacaaagcga
     1201 taaattaatc aaggcccgtt gacgtgcgct tttccccaga cgcccaacca gcatgcccaa
     1261 ttttcccaac aatggatctc tcagtgcggg ggggaccatt gcaaacacac agataatacc
     1321 gatacctgtc cacgtcatcc agtaacgggg gaagcataat gtaaggttaa agcggggaat
     1381 aaactcagtt ctgatatttt tcatatttac cggtatctgc ctgcttatct cattatgata
     1441 acagagtaca gcagaaggtg gtaccatccc tggagtccac cggacagcag aggaaaacac
     1501 atcctcctgc cttcagaacg gaaccgaaga taagaaaata ctatggctca ccatacggaa
     1561 gagtgtcgaa atcagtgagc ccattctttt tatacggatt tccaatccag cgggtagttt
     1621 ccctgaattg cggattcacc agagaacggg tcggatcgta accatcgtat gtcagtcccg
     1681 ccaaatctga ccaggtgtgg atcagttcag ccagactgta tttgcggtca acatactgcg
     1741 aaaaatctcg cggatgtgca gcatgccact tttccgaggt ccacagcagg aacggaacag
     1801 tgtacatggg acgtgtgggg ttatcttcat tccgtccctg cgttttatat ggcggagtat
     1861 catatacttc ttcaccatga tctgaaaaat aaagcagaaa gccgtccggc gcagttgctc
     1921 tgaagtcctt tatcagactg gccaccacat gatcgttaaa cagattggca ttatcgtaat
     1981 cgttatacac ttccagctct ttcgcattta atccagtggg aatatgccct gtaatgccat
     2041 caaaccgccc ctgaccttcc ggataacggt atttatactt aatgtgcgta cccagcagat
     2101 gcacgatgat cagtttcttt ggtgcagggt cattcagcac ttcccggaac ggcttcagca
     2161 cgttagtgtc atattcacgc gcactttgtg ttcgctgctg attcatgtag tactgcctgt
     2221 ccgtctggcg cgaaaataca gtgagcatgg tgttacgggc tgtgattgtc tgctggttgg
     2281 taatccagaa tgttttatag cctgcctgct tcatcatgtt catcagcgac ggctgcgtca
     2341 gatacagatc aggattcttt tcgttggcga aagtaagggc ctgttgcaat gcttcaatgg
     2401 tgtacggacg cgatgccacc acattattaa acacagtaag acccggatcg gttttacgca
     2461 gtgcatccag ctccggcgtc gtttcacgta gatacccgta taagctcatg cgttcgcgct
     2521 gtgtcgactc accaatcacc aggaccagtg tgcgtggtct ctcccctgat tcatccctga
     2581 gattacccag tggtggcagt gcgctatttt cattcaggaa ggttgtcagt gcattcagtt
     2641 gctgatggta ctgataataa ctggatacaa actgccaggg ggcagcaggc tccattcggg
     2701 atgccagttt gcctagagta tcattcagcg gctcctgtct gattaacgat ttcagaacaa
     2761 ccggatgcag aagcagagca taaagcagca ggaaagagac aattctccgc catggcaaag
     2821 gaatatatac aggacgcaga cgtgtccaca gaaaaacgga caccgcagta tataccagcg
     2881 agataagcaa cagtttaagg ctgaaatact ggctgaaata ttcaccagcc tctctggcat
     2941 tcgtttcaaa cataacgaaa agaacacttt gagagaattc atgaccatag agaaaataat
     3001 aacaaagtgc tgccagcgac gtgccccaga gaatgaaacc gacaacagct gcaattattt
     3061 ttatccgatc aggatagaga aacaccggga tcaaccacag acaactgaat aacagtgagt
     3121 cccgtattcc attcgttccg ctgtacccac tgctgaagat gatgacctgc aggagagtgg
     3181 aaaaaaagcc aaaataaaaa attgcccatc ccagcgcact ccagctgaaa gtaggcctgt
     3241 tctgtccggt atttaaatgc attgaccgtc cccgtattta aacaatgtga taaattactc
     3301 cgttacttgc cggaaaaccg ctgaacaaaa ttcgggctga aaagaggatc cgccgttatc
     3361 tgttacattt ccccttagcc tgactagcca gagacacaat gatctgtgcc gttctgttaa
     3421 tatcaaaccg gtactcaata tcttctctgg cgctggctgc catcatccgg aagcgttccg
     3481 gtcgggataa aaaatcgcgc agtgcgccgg tccatgcaga cacatccccc acgggtaaca
     3541 gcgtccctgt cacattcttc tgaatgacat cagggatccc gcccgtctca ctggcgataa
     3601 cgggcacgcc agagactgac gcttcagcca gtaccatacc aaacgcttca ttttccgaag
     3661 gcatgaccac cacactggca atccggtaga ccggtaacgc tgggaaaagg gcacctgcca
     3721 ttaacacatc tccgctcatt cccaggtgtt ctgtctgctg acgcagacgt gcttcgtatt
     3781 cttcacgccc ggcgcccacc acgagccagc gaaatgattt cccttccatc ttcagctgat
     3841 acaatacacg cagcataaat tcatgtcctt tttcgggacg tagcatcccc acctgaacga
     3901 taagcggaac attgtctgct gatgcagccc aggcgtggat atgcaggggt aacggtcgca
     3961 tggcttcatt atgcaatgcg ggccagtcga aacccggtgg aataaccgtt accggtgtcc
     4021 tgacaccttc cgccatcaga tgcgccatca tggctgagct aggcacaaca atgaaatcac
     4081 acagataatt cagggaaaac gttcgggtct tacgggtgat gtaggttttt tgtctgacaa
     4141 tactgaagcg gtgacagcat atcagacggc tcagtcctgc tatattactg tcatggccac
     4201 tatggcagat gaccagatca ggtttaaatt ccccgataat ccgtcgaagt ctgaggatgg
     4261 aaggaaggtg aaggctgttc ctgaaaggaa taaaagtgac atcatgccct ctttttctgg
     4321 cttccggagc aattttactt ttttccctgc aggcaagtaa aacggaatgt ccctgctttt
     4381 gaagagcagt catctgggcc agtgcctgca gctcctggcc cccaatatct gacgaagatt
     4441 cagtgaataa aattttcatc attaattatc tggtaatctt ggccccttat gaagagccag
     4501 tactgaagaa aaaagtggcg ttgtataata aaaaaggcgt cgtttcagcc agccggaatg
     4561 ttcccgctct ttggtgctga tacgccctat gcgtaatgaa ccattttcag gacagttcat
     4621 tcttctttcg gttgtatata aataagagaa accaagctta ccggcgagat taatataatc
     4681 ccgattataa tatccttcag gccagcacag atgggaacta cagaacccca gcttttccgt
     4741 cagacattgt tttcccacca gaatatcttc tttcatgagc cggcactgtt cagcacgtga
     4801 tacagacaac ctgtcccagc gtttatgtga atgggtgtgc acatgaaatt caaccagtcc
     4861 ggaatcacgc atttcccgga cttcagacca gcgaagcatg acctcatctg aacggttatc
     4921 ggcaatcaac cgttcacagt cgcggtgaga atattcctgc tcctgcctgc tacgtacgtt
     4981 tcccttacca atcagccccg taatgagaaa aatatgtgca tgaaggttat attccttcag
     5041 gaccggccat gctctgagcc agttatcaag ataaccatca tcgaatgtga gcatgacact
     5101 ctttcgtgga agcgttcccc cctgataaaa atattcaagc tcagcagatg ttaccgtccg
     5161 ccagttatta tctgcgagcc acttcatctg ttcacaaaat gtttcaggtg agagtgtcac
     5221 aagccccgga caacgactga catgatgata catcagtacg ggaagatgcc ttgcattcaa
     5281 cataaaaaat tatcgtctgt taagcattct gaaaatatcc tcgcgtaaac aacagtgagg
     5341 atccatagat atcacacatg gtgatattat tgtgtaatcc cggaatggtc cggagattac
     5401 cgacaacagg gattttttaa tatttttatc agttaatcaa ccagaactta aatttccctt
     5461 aacgcatatg ctctttttaa tcagattttc tgtttttcag aaaaaacaga ataccgcaat
     5521 ttcagtaaac acagcgagag atatccgcct cagtaaaaaa tcagaagatt atcatcctga
     5581 tttattgcat aaaaccagtc gagtagcgga attttctgca tccggatacc actccccctg
     5641 aaaaatactc gcacaatatc gcgccgcatg cactgatgga ccgggccatc gtgagaatct
     5701 cttgcatcgt agatattaac cacaccagtg ccccccagaa caacagtaat acctgtagca
     5761 gacctgccat agaggttaaa taaccattca tatccctctt ctacccattc atcccgaagt
     5821 ttcagccagc gacgaatgga ctgtacctcc tgtttcagcc gaggggctga gttgacaaac
     5881 cgtttagagc cgccggatat tcaggttacc ggcctgcaaa tcaatatcag acagttttat
     5941 acctaataac tctgatgctt gtacaccatg aagatacccc ataaggagca ggcagtgatt
     6001 acgttccggg tgtggcactt tgaagacagc actgtgcaac ttatgaatct cacccgggtt
     6061 cagatatttt tgttgtccat tacctgtcct tatttattga aagtcgatat tagtttaaaa
     6121 agctgctaat catgacacca ttacagaagt aaaattaatt tattttaaat acataaaatt
     6181 attgttcatt tatttttttt gcgaacattc ataaactaaa aaacaatgga taaaaccaat
     6241 aacattgcat aataatacac ctcccttata aataatggag aagaaaatga aaaggcggta
     6301 tatcacctat gctgaacctg tttaggatgc tgtgtaaatg ccttttctcc gaagtgaccg
     6361 tccaagcggt caccgaattc gataataaag cggctcattg ccatacacca gtccttcaac
     6421 ggcatcgtcc atttctgagg cgcagactgg atagctaaag tgtagcggta ttaggagctg
     6481 ttatttagtt cgaatatcga aaatttttgt gtacgttggt cgtatcaatc tctttttatt
     6541 tgaattagtt tgataacaga tttttgcaat ctgataactc atttatttat tgagggataa
     6601 atgcaatatt gtacaatatg tttccgccat tagcaaaaga atccacaagg cgcttgcact
     6661 aatgcatggt aatgcctcca attagttgaa ttaggggtat atgaaccgcc ccgggaatcc
     6721 tggagactaa actccctgag aaagaggtaa acaggatgac taaaaataca cgtttttccc
     6781 ccgaggtccg tcaacgggca gttcgtatgg ttctggaaag tcagggcgaa tatgactcac
     6841 aatgggcggc aatttgttcc attgccccaa agattggctg tacaccagag actctgcgtg
     6901 tgtgggttcg tcagcatgag cgggataccg ggagtggtga tggtggactc accaccgctg
     6961 aacgtcagcg tctgaaagag ctggaacgtg aaaatcgtga actgcgccgc agtaacgata
     7021 tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc tggaaaaaat
     7081 aatgcctctg ctggataagc tgcgtgagca gtacggggtc ggaccggtat gcagtgaact
     7141 gcatattgcc ccgtcaacgt attaccactg tcagcaacag cgacatcatc ctgataaacg
     7201 cagtgcccgt gcgcagcgcg atgactggct gaagagagag atacagcgcg tatacgatga
     7261 aaatcatcag gtatacggtg tgcgtaaagt ctggcgccag ttgttacgcg aaggtatcag
     7321 ggtggccaga tgtacagtgg cgcgcctcat ggcggttatg ggacttgccg gtgttctccg
     7381 gggtaaaaag gtccgtacta ccgtcagccg atttacgggc cgtaagtaac gaagttggat
     7441 gcaaatgtca gatcgtgtgc gcctgttatg gctcggctgg taagagagcc ttataggcgc
     7501 atttgaaaaa actccagcta taccggaaga tatttattat taacaggaga ggtttgcttt
     7561 tctgtgtagg tttagatttt gtgtttgcag ggtaaacaaa actgattaac tcgtcatttc
     7621 ggtaactgaa atagtttgct gtattctgtt tacaatctct ttaataaggc atttgtcacg
     7681 tggctattca tatgaaaatc atacgttatc ttacaaatca atcatctgag tgttataata
     7741 taacttagct gtgatatgtg taagaatgtt taggcaatat attattatcg ctaatgttgt
     7801 tttggttggg agagattctc cttgcggtag ctttttgttt ttagatgctt cttgcttaaa
     7861 aagatataat ttctgttctt ttatatagga ttctttacat ttactgagtt ttcttattta
     7921 tttaaacatt atgaaatcta atgcttttga cgtcatgggg aaggttgcat ggctatgggc
     7981 ctgttctcct ctgcataaaa aatggccatt atctgttttt gctattaatg tcatacctgc
     8041 tattcagaca aatcaatttg ctctcttaat aaaggatgag cttcctgtag ctttttgtag
     8101 ctgggccagt ttagatctgg aatgtgaggt aaaatatata aatgatgtaa catctttgta
     8161 tgcaaaagac tggatgtcgg gagaaagaaa gtggtttatt gactggatag ctccatttgg
     8221 acacaacatg gagttatata aatacatgag gaaaaagtat ccttatgagt tattcagagc
     8281 aataaggtta gatgagagca gtaaaacagg aaaaatagct gaatttcatg gaggggggat
     8341 agataaaaaa ctggcatcaa aaatattcag gcaataccat catgaactta tgtctgaagt
     8401 aaaaaacaga caagatttta attttaatat tgagaaagaa aactaatatg acagtaaata
     8461 aaataaagaa cattttcaat aatgcgacat tgactacaaa atcagcattt aatacagcat
     8521 catcaagcgt acgttccgct ggcaaaaaac tcatattatt aatacctgat aattatgaag
     8581 ctcagggcgt gggtattaat gagttggtca aagctgctga tgagcttgga atagaaatac
     8641 accgtactga acgagatgat acagcgattg caaaccagtt ttttggtgca gcagaaaaag
     8701 ttgtaggatt aactgaacgt ggtgttgcaa tattcgcacc acaacttgac aaacttctgc
     8761 agaagtatca gaaagttggg agtaaaatag gaagaaccgc tgaaaatgta ggtaataatc
     8821 tgggaaaagc cggaacagtt ctctcagcac tacagaattt tacggggatt gctttatcag
     8881 gcatggctct tgatgaattg ctgagaaaac aacgggaagg agaggatata agtcagaatg
     8941 atattgccaa aagtagtatt gaacttatta atcagcttgt agatacagta tcaagtataa
     9001 acagtaccgt tgattcattt tctgagcagc ttaaccagct tggctcattt ttatccagta
     9061 aacctcgctt aagttctgtt ggtgggaaat tacaaaattt accagacctg ggctccctgg
     9121 gggatgggct ggatgttgtc tccggaattc tttctgctgt atcagcaagc tttattctgg
     9181 gaaacagtga cgcacataca ggaacaaaag ctgcagcggg tatcgaactg acaactcagg
     9241 ttcttggaaa tgttggtaaa gctgtttcgc aatatattct ggctcagaga atggcacagg
     9301 ggttatcgac aacagctgca agtgcgggtc tgatcacatc ggctgttatg ctggctatca
     9361 gtcctctttc tttcctggct gctgcagata aatttgagcg agctaagcag cttgaatcat
     9421 attctgaacg atttaaaaaa ttgaattatg aaggggatgc tttactcgca gcctttcata
     9481 aagaaaccgg agctatagat gcagccctga caacaataaa tactgtcctg agttctgtat
     9541 ctgcgggagt tagtgcagcc tccagtgcat ccctcatagg ggccccgata agcatgctgg
     9601 tgagtgcatt aaccggtacg atatctggca ttctggaagc atcaaaacag gctatgtttg
     9661 agcacgttgc agagaaattc gctgctcgga tcaatgaatg ggaaaaggag catggcaaaa
     9721 attattttga gaatggctat gacgcaagac atgctgcgtt tttagaagac tctctgtctt
     9781 tgcttgctga tttttctcgt cagcatgcag tagaaagagc agtcgcaata acccagcaac
     9841 attgggatga gaagatcggt gaacttgcag gcataacccg taatgctgat cgcagtcaga
     9901 gtggtaaggc atatattaat tatctggaaa atggagggct tttagaggct caaccgaaag
     9961 agtttacaca acaagtgttt gatcctcaaa aagggaccat agacctttca acaggtaatg
    10021 tatcaagtgt tttgacattt ataacaccaa catttacccc aggagaagaa gttagagaaa
    10081 gaaaacagag tggtaaatat gaatatatga catctcttat tgtaaatggt aaggatacat
    10141 ggtctgtaaa aggcataaaa aatcataaag gtgtatatga ttattcaaaa ttgattcagt
    10201 ttgttgaaaa gaataacaaa cactatcagg cgagaatgat ttctgaactc ggagataaag
    10261 acgatgtggt ttattctgga gcaggctcat cagaagtatt tgctggtgaa ggtcatgata
    10321 ccgtatctta taataagacg gatgttggta aactaacaat tgatgcaaca ggagcatcaa
    10381 aacctggtga gtatatagtt tcaaaaaata tgtatggtga cgtgaaggta ttgcaggaag
    10441 tcgttaagga acaggaggtg tcagtaggga agcgaacaga gaaaatacaa tatcgtgatt
    10501 ttgaattcag aaccggtgga attccttatg atgtaataga taatcttcat tctgttgaag
    10561 agctcattgg cggaaaacat gatgatgaat tcaaaggcgg taagtttaat gatatattcc
    10621 atggcgcaga tgggaacgat tatatcgaag gtaattatgg taatgatcga ctatacggcg
    10681 atgatgggga tgattatata tccggaggac agggagacga ccagttattt ggtggtagtg
    10741 gaaacgataa attgagtgga ggggatggta ataattatct gacaggagga agcggtaatg
    10801 atgagcttca ggcacacgga gcttataata ttctgtcagg tggtactggt gatgataaac
    10861 tttatggtgg tggtggtatt gatcttctgg atggagggga aggtaatgac tatctgaatg
    10921 gtggttttgg taatgatatt tatgtttata ggcaaaacta tggtcatcat acaattgcag
    10981 atgaaggagg taaaggagat cgtttgcact tatctgatat tagctttgat gatatcgcat
    11041 ttaagagagt tggaaatgat cttatcatga ataaagccat taatggtgta ctttcattta
    11101 atgagtcaaa tgatgtcaat gggataacat ttaaaaactg gtttgcgaaa gatgcctcag
    11161 gagcagataa tcatcttgtt gaggttataa cagataaaga tggtcgagag ataaaagttg
    11221 ataaaatacc tcataataat aatgaacggt caggttatat aaaagccagt aatatagcat
    11281 ctgaaaaaaa catggttaat atcaccagtg ttgccaatga tattaataag attatttctt
    11341 cagtttcagg gttcgattca ggtgatgaac gattagcatc tttatataat ttatccttac
    11401 atcaaaacaa cacacactca acaactttaa cgacaactgt ctgagactaa aagaggaggt
    11461 gatggccatt ccaggaggaa tggtcatcgt tatatgatga gtaaatgtag ttctcataat
    11521 agtctgtatg cactgatatt gcttgcacaa tatcataata taactgtcaa tgctgaaact
    11581 ataaggcatc agtataatac ccacacacaa gattttgggg tgactgaatg gttactggca
    11641 gcgaaatcca ttggcttaaa agcaaaatat gtagaaaaac atttttccag attgtcaata
    11701 atttctttac ctgcgttgat atggcgggat gacggtaagc attatatatt gtctcgtgtt
    11761 actaaagatt catcacgcta tcttgtttat gatccagaac aacatcagtc actaactttt
    11821 agtcgggatg agtttgaaaa actgtatcag ggaaaagtca ttctggttac gtcaagagca
    11881 acagtagtcg gagagttagc taaatttgat ttttcttggt ttatcccctc tgttgtgaaa
    11941 tacaggagga ttttacttga ggtgttaact gtttctgctt ttattcagtt tcttgcgtta
    12001 ataacacctc ttttttttca ggttgtaatg gataaggttt tagttcaccg ggggttttca
    12061 acgttaaata ttatcacaat agcatttatt atagtgatac tttttgaagt gatattaacc
    12121 ggagccagaa cttatatttt ctctcatact acaagtcgta ttgacgtcga actgagcgct
    12181 aagttattca gacatttgct tgcattgcct gtttcatatt ttgaaaatcg cagggtcgga
    12241 gagaccgttg ccagagtaag ggaactggag caaattcgta attttttaac cggacaagcg
    12301 ttgacatcag ttcttgatct atttttttct gtaatatttt tttgtgtcat gtggtattac
    12361 agccctcaat taacactggt tatattattg tcactacctt gttatgttat atggtcattg
    12421 tttatatcac ccttattacg tcgacgtctt gatgataagt ttctcaggaa tgcagaaaat
    12481 caagcttttc ttgtcgaaac ggtaacagca ataaatacaa ttaaatccat ggcagtatca
    12541 ccacagatga ttgctacatg ggataaacaa ctggccgggt atgttgcttc cagtttcagg
    12601 gtaaatctgg ttgcaatgac agggcagcag gggatacagc tgatacagaa aagtgtaatg
    12661 gtaattagtc tatggatggg agcacatctt gtcatatcgg gagagataag tatcgggcag
    12721 ttaatcgcgt ttaatatgct tgcaggtcag gtcattgctc ctgttatcag actggctcat
    12781 ctttggcagg attttcagca ggttggtatt tctgttgagc gccttggtga tgtattaaat
    12841 acaccggtag agaaaaagtc aggcagaaat atactgccgg aaattcaggg ggatatcgaa
    12901 tttaaaaatg tcagatttcg gtattcttct gacggtaatg ttattttgaa taatattaat
    12961 ttatacatat caaaggggga tgttatcggt atagttggcc gttctggttc aggaaaaagt
    13021 acattaacaa aactgcttca gcgcttttat ataccagaga ccggacagat tttaattgat
    13081 gggcatgatt tatcacttgc agatccagaa tggttacgac gccagattgg tgttgtattg
    13141 caggaaaata tactattaaa tcgtagtatt atcgataata ttacattagc ttctccggct
    13201 gtatctatgg aacaggctat tgaggcagcc agacttgcag gtgcccatga ttttattaca
    13261 gaactaaaag aagggtacaa tactattgtt ggagaacagg gcgttggtct ttcaggaggg
    13321 cagcgccaac ggatcgcaat agcccgggct cttgttacaa atcctcgaat tcttattttt
    13381 gatgaagcaa ccagtgctct tgattatgag tcggaaaata taataatgaa aaatatgtca
    13441 agaatatgta agaacagaac cgtaattatt attgcacaca ggttgtcaac tgtaaaaaat
    13501 gcaaacagaa taattgttat ggataatggc tttatttctg aagatggcac acataaagag
    13561 cttatctcca aaaaagacag tttatatgca tatttatatc agttgcaggc ataacgtatg
    13621 cgcttttaca tgaaaggttt atgggatttg gtttgccgtt ataagacggt tttttctgat
    13681 gtctggaaaa ttcgtcacac acttgatgct cctgtgagag aaaaagatga gtatgcattt
    13741 cttcctgctc accttgaact cattgaaaca ccagtatcca gacgttctca ttttgttgta
    13801 tggagtattt tattatttgt aattatatct cttctcttat ctgttcttgg gaaagttgaa
    13861 gtggtttcag tagcaaatgg taagtttact catagtggaa gaagcaaaga aataaaaccg
    13921 atagagaatg cgattgtaga aaaaataatg gtaaaagatg gctcttttgt aaaaaaaaat
    13981 gatccattag ttgaattgac ggtgcctggt gttgaatctg atattttaaa atcagaagca
    14041 tctttgttgt atgaaaaaac agaacaatat cgctacgcaa ttctttctga atcaatacaa
    14101 cggaatgagc ttcctgaaat aaggataaca gattttcctg gcggagaaga taacgcggga
    14161 ggtgaacatt ttcagagggt tagctcatta ataaaagagc aattcatgac atggcagaac
    14221 agaaaaaatc aaaagcagct cacattaaat aaaaaaatag ttgaacggga tgcagcgctt
    14281 gctcgtgtta gcctgtatga gcatcaggta tcacaggaag gaagaaaact caatgatttt
    14341 aagtatttgt tgaataaaaa agctgtttct caacattcag ttatggagca ggaaaatagc
    14401 tatattcagg caaaaaatga acatgcagtc tggcttgcac aggtttctca acttgaaaaa
    14461 gaaatagaac ttgtgcggga agaactggca ctggagacga atatcttcag aagtgaaatt
    14521 atcgagaagc acagaaaatc aacagataac attgtgttgc tggagcatga gcttgaaaaa
    14581 aacaggcaga gaaaagcatc atcttttatt aaagctcctg tgagtggtac tgttcaggag
    14641 ttaaatatac atacagaagg tggtgttgtc acaacagcag aaacgctgat gattattgtc
    14701 cctgataatg atattctcga agtaacagca tctgtactta acaaggatat cggttttata
    14761 caacctggac aggaggtcgt tattaaagta gatgcatatc catatacacg tcatggttac
    14821 cttacaggga aagtaaaaaa tataactgca gattctgttt ctgttccgga tacagggctt
    14881 gtatttaacg tgattatatc tgttgatcgg aatgatatac agggagaaag aaaaaaaatc
    14941 cctgttacag ctggaatgac tgttatggca gaaataaaaa ccggggtgcg tagtgttat
//
LOCUS       DAZGOB010000126        14976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGOB010000126 DAZGOB010000000
VERSION     DAZGOB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.126"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(44..2323)
                     /gene="maeB"
                     /locus_tag="ACHLIF_004006"
     CDS             complement(44..2323)
                     /gene="maeB"
                     /locus_tag="ACHLIF_004006"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO9607919.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2612..3562
                     /gene="tal"
                     /locus_tag="ACHLIF_004007"
     CDS             2612..3562
                     /gene="tal"
                     /locus_tag="ACHLIF_004007"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO9607920.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3582..5585
                     /gene="tkt"
                     /locus_tag="ACHLIF_004008"
     CDS             3582..5585
                     /gene="tkt"
                     /locus_tag="ACHLIF_004008"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO9607921.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSMKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGVIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5681..6724)
                     /gene="ypfG"
                     /locus_tag="ACHLIF_004009"
     CDS             complement(5681..6724)
                     /gene="ypfG"
                     /locus_tag="ACHLIF_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO9607922.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQVIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6850..7425)
                     /gene="nudK"
                     /locus_tag="ACHLIF_004010"
     CDS             complement(6850..7425)
                     /gene="nudK"
                     /locus_tag="ACHLIF_004010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO9607923.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7493..9472)
                     /gene="aegA"
                     /locus_tag="ACHLIF_004011"
     CDS             complement(7493..9472)
                     /gene="aegA"
                     /locus_tag="ACHLIF_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO9607924.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKPRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMNAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9678..11378
                     /gene="narQ"
                     /locus_tag="ACHLIF_004012"
     CDS             9678..11378
                     /gene="narQ"
                     /locus_tag="ACHLIF_004012"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO9607925.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11542..14655
                     /gene="acrD"
                     /locus_tag="ACHLIF_004013"
     CDS             11542..14655
                     /gene="acrD"
                     /locus_tag="ACHLIF_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO9607926.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14754..14813)
                     /gene="ypfM"
                     /locus_tag="ACHLIF_004014"
     CDS             complement(14754..14813)
                     /gene="ypfM"
                     /locus_tag="ACHLIF_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO9607927.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3514 a         3977 c         3910 g         3575 t
ORIGIN      
        1 cttaccctga atattcaggg taagcgtgag agttaaaaaa gaattacagc ggttgggttt
       61 gcgcttcaac cacggccagc gccaccatgt tgacgatacg acgcaccgat gcgatcggcg
      121 ttaacacgtg aaccggtttc gcaacgccca tcagcaccgg accgacagtc acaccttccg
      181 agctggaaac gcgcagtaag ttgtaactaa tgcgggcagc ttccatgttc ggcatcacca
      241 gaatattggc agaacctttc aaagggctgt ccggcatacg gtcgttgcga atcgcttcca
      301 ccagcgctgc atcgccgtgc atttcaccat caatcatcag atctggcgca cgatccctga
      361 ccagttccag cgcctgacgc attttgctcg acgacgggca gtcagaagaa ccaaagttgg
      421 agtgcgacaa cagagcaacg cgcggctcaa taccaaaacg acggacagtt tctgccgcca
      481 tcaaggtgat ctccgccagc tcttctgcgt ctggttcgtc attgacgtag gtatcggcaa
      541 taaaggtgtt accactcggc agcagcagcg cgttcattgc acctgcggtg tgaacgccat
      601 cgcgataacc aaagacattt ttcaccacgc taaaatgttc atgataatcg cccaccgtac
      661 cgcagatcat tgcatcggct tccccacgct gaaccatgat cgcgccgatc actgtcgggt
      721 tactgatcag cgcccgctgc gcctgttcct gagtgacacc gcgacgcttc atgatctgga
      781 agtattcggt ccagtactct ttaaagcgcg gatcggattc gttattgacg atctcaaaat
      841 caacgcccgc tttgatctgc aagcccagtt tctgaatgcg catttcgatc acgttcggac
      901 gaccgataag gatcggtttc gccagtccca gcgttaccag ttcctgagtg gcatgcagaa
      961 cgcgcgcctc ttccccttct ggcagaacaa cgcgcttcgg cgctttgcga gcctgggaga
     1021 aaatcggctt cataaacagg ttggttttgt aaacgaactc agtcagcttg tcgatgtaga
     1081 cgtcgaaatc agcaatcgga cgcgtcgcca cgcccgactc catcgcggct ttagcgaccg
     1141 caggagcgat cttaacgatc aagcgcggat caaacggttt tggaatgatg tattccggac
     1201 caaagctcag atcctgatcg ccatacgctg aagccaccac ttcgctctgt tcggcgtggg
     1261 ccagttccgc aatcgcgcgt accgccgcca gtttcatctc ttcattgata gccgttgcgc
     1321 caacatccag cgcgccacgg aagataaacg ggaagcaaag gacgttgttg acctggtttg
     1381 gatagtcaga acgaccggtg caaatgatgg catccggacg cacttctttc gccagcggcg
     1441 gcagaatttc cggttccggg ttcgccagcg ccaggatcat tggcgcacga gccattttct
     1501 tcaccatttc ctgggtcagc actttcgggc cggaacatcc aaggaaaata tccgcgcctt
     1561 caatcacatc atcgagggta cgtttgccgt catccaccac cgcatatgcg gctttggttt
     1621 ccgccatgtt tggctcacgc ccctgataga taacgccttt tgaatcgcaa accacgatgt
     1681 tatgtttttg cagacccagc gctaccagca ggttcataca ggcgattgct gcggcacccg
     1741 cgccggaaac caccatccgc acgtcggaga tgtttttctc caccacgcgc aagccgttga
     1801 ggatggcggc agtgctgatg attgccgtgc cgtgctgatc gtcgtggaat accggaatat
     1861 tcatccgctc gcgcagtttc tgttcaatat agaaacattc tggcgcttta atgtcttcga
     1921 ggttgattcc gccgaaggtc ggttcgagcg cagcgacaac ttcaataaat ttgtccgggt
     1981 cgagttcatc aacttcaatg tcaaatacat caatcccggc gaatttctta aacagaacgc
     2041 ccttgccttc catcaccggt ttgccagcca gcgcgccaat gttgcctaac cccagcaccg
     2101 ccgtaccgtt agagatcacc gccaccaggt taccgcgcgc ggtatatttg taggctttta
     2161 acgggtcttt ttcgatttca agacaaggtg cggcaacgcc tggtgagtag gccagcgcca
     2221 gatcgcgctg cgttgccaga ggcttggttg gagaaacctg gattttccct ggaactggaa
     2281 attcatggaa atcaagtgca ctttgtttta actggtcatc catttggttg ttcctttcac
     2341 gtaacgttca caaataaagt gtgtgggcaa cagcccctgc ccacaacgtg gcgcacatta
     2401 ttaccctgcc ggagtctaca gactttgagc aagtccaaac tctcaccatt aatataatgt
     2461 tttggtaata atcctataac actgatgtta cctgcttaat ccagcaatac catgcctgtc
     2521 tgctatgctt ttttgatgcg tttagcgaaa tttctcagaa gtgtgaatta acgcactcat
     2581 ctaacacttt acttttcaag gagtatttcc tatgaacgag ttagacggca tcaaacagtt
     2641 caccactgtc gtggcagaca gcggcgatat tgagtccatt cgccattatc atccccagga
     2701 tgccaccacc aatccttcgc tgttactcaa agctgccgga ttatcacaat atgagcattt
     2761 aatagacgat gctatcgcct ggggtaaaaa aaatggcaag acccaggaac aacaggtggt
     2821 cgcagcgtgt gacaaactgg cagtcaattt cggtgctgaa attctcaaaa tcgtacccgg
     2881 tcgcgtgtca acagaagttg atgcacgcct ctcttttgat aaagaaaaga gtattgaaaa
     2941 ggcgcgccat ctggtggact tgtatcagca acaaggcgtt gagaaatcac gcattttgat
     3001 caaactggct tcgacctggg aaggaattcg cgcggccgaa gagctggaaa aagaaggtat
     3061 taactgcaac ctgacgctgc tgttttcttt tgcccaggcg cgggcctgtg cagaagcggg
     3121 cgtttttctg atttcgccgt ttgtcgggcg tatttatgac tggtatcagg cacgcaagcc
     3181 gatggacccg tatgtggtgg aagaagatcc gggcgttaaa tcggtgcgca atatctacga
     3241 ctactataag caacaccact atgaaaccat tgtgatgggc gcgagcttcc gtcgcaccga
     3301 acaaatcctc gccttaaccg gctgcgatcg actgactatc gcaccgaatt tgctgaagga
     3361 gctgcaggaa aaagtttcgc cagtggtacg taaattaatc ccgccttctc agacgttccc
     3421 acgcccagca cccatgagcg aagcggagtt ccgttgggag cacaatcagg atgcgatggc
     3481 ggtagaaaaa ctgtctgaag gcattcgtct gttcgctgtt gatcaacgca aactggaaga
     3541 tcttcttgcc gccaaactat aaaccagcca cggagtgtta tatgtcccga aaagaccttg
     3601 ccaatgcgat tcgcgcactc agtatggatg cggtacaaaa agccaattct ggtcatcccg
     3661 gcgcgccgat gggcatggct gatattgccg aagtgctgtg gaacgatttt cttaaacata
     3721 atcctaccga cccaacctgg tatgatcgcg accgctttat tctttccaac ggtcacgcgt
     3781 cgatgctgct ctacagtttg ctacatctga ccggttacga cctgccgctg gaagaactga
     3841 agaacttccg tcagttgcat tcgaaaaccc caggccaccc ggagattggc tatacgccag
     3901 gcgttgaaac caccaccggc ccgcttggac aaggtttggc gaacgccgtc gggctggcga
     3961 tagcagagcg tacactggcg gcgcagttta accagccaga ccatgagatc gtcgatcact
     4021 tcacctatgt gtttatgggc gacggctgcc tgatggaagg tatttcccac gaagtctgtt
     4081 cgctggcagg cacgctggga ctgggcaagc tgattggttt ttacgatcac aacggtattt
     4141 ccatcgacgg tgaaacagaa ggctggttta ccgacgatac ggcaaaacgt tttgaagcct
     4201 atcactggca tgtgatccat gaaatcgacg gtcacgatcc gcaggcggtg aaggaagcga
     4261 tccttgaagc gcaaagcatg aaagataagc cgtcgctgat tatctgccgt acggtgattg
     4321 gctttggttc gccgaataaa gcaggtaagg aagaggcgca cggcgcacca ctgggggaag
     4381 aagaagtggc gctggcacgg caaaaactgg gctggcacca tccgccattt gagatcccta
     4441 aaaagattta tcacgcctgg gatgcccgcg aaaaaggcga aaaagcgcag cagagctgga
     4501 atgagaagtt tgccgcctat aaaaaggctc atccgcaact ggcagaagag tttacccgtc
     4561 ggatgagcgg tggtttaccg aaggactggg agaaaacgac tcagaaatat atcaatgagt
     4621 tgcaggcgaa tccggcgaaa atcgccaccc gtaaggcttc gcaaaatacg cttaacgctt
     4681 acggaccgat gctaccggag ctgctcggag gttcagcgga tctggctccc agcaacctca
     4741 ccatctggaa aggctctgtg tcgctgaagg aagatccggc gggcaactac attcactacg
     4801 gggtgcgtga atttggcatg accgctatcg ccaacggcat tgcgcaccac ggcggctttg
     4861 tgccgtatac cgcgacgttc ctgatgtttg ttgaatacgc ccgtaatgcc gcgcggatgg
     4921 cggcactgat gaaagcacgg cagattatgg tttataccca cgattctatt ggcttagggg
     4981 aagatggtcc gacgcaccag gccgttgagc aactggccag tctgcgctta acgccgaatt
     5041 tcagcacctg gcgaccgtgc gatcaggtgg aagcggcggt gggctggaag ctggcggttg
     5101 agcgccacaa cgggccgaca gcactgatcc tctcaaggca gaatctggcc caggtggaac
     5161 gtacgccgga tcaggttaaa gagattgctc gtggcggtta tgtgctgaaa gacagcggcg
     5221 gtaagccaga tattattctg attgccaccg gttcagagat ggaaattacc ctgcaagcgg
     5281 cagagaaatt agcaggagaa ggtcgcaatg tacgcgtagt ttccctgccc tcgaccgata
     5341 ttttcgacgc ccaggatgag gaatatcggg agtcggtgtt gccttctaac gttgcggctc
     5401 gcgtggcggt ggaagcaggt attgccgatt actggtacaa gtatgttggt ctgaaagggg
     5461 taattgtcgg gatgacgggt tacggggaat ctgctccggc ggataagctg ttcccgttct
     5521 ttggctttac cgccgagaat attgtggcaa aagcgcataa ggtgctggga gtgaaaggtg
     5581 cctgatggtg atcgccggat gctgattgcc ggatgcgacg ctgacgcgtc ttatccggcc
     5641 tacagcgtct tatccggcct acatgtcccc gcaattttgt ttaacgggtg atccacaacg
     5701 tgggccaggc gtctggccca tgccagttat cgcaggtggg ttctgcggca taacgcacca
     5761 ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg cttaatccgc
     5821 gccctttcgc taaggtcacc agttcacgcg atttctcatc aaatgttgcg ttcatcagtt
     5881 ccagttcatt cgtctcctga ccgttgttga atggcaaacg caaccgaacc gggcgcgagg
     5941 ctagtggctt tttacgcgac acaatccatg ccaaatcaat ggtgttatag gcccctgcct
     6001 cacagctaat catcatcagc gctttatcat cagtcagcgc agtgacattc acctcacgac
     6061 gtaacggatc aagcgagcag cgcagaccat tcatccgcca gtttccataa tccagcaaat
     6121 cgttgcgttc ttcgagtgag agtggcgtcg gcgttgggtt aaccaccgcg acctctttca
     6181 gcgcaggcgc gggcggtacg ctgagcggcg gttcgtcccc tttcttgatc cacgcggttt
     6241 cactgccaac gcgcttttgc tgagcatcaa taaacaacaa cgccgctttt aagccactca
     6301 gagaaatggt ctgattgccg tcgcgtaagg tgatcgcctt cccttcctga atcacctgca
     6361 aaaacgcggt gatggttgcc gtatcatcgg ttactaataa ccatggtgaa atccgccact
     6421 tgtcaccact taacgccaac ggctcaccat ctaacagcag ccgtggcgct atctctcctt
     6481 cagacgcatc cggcgacttc aacccgccgc gctcaatacg taaaacggca tcggtatgcg
     6541 ccccggcgct gcggctcagg gtcatcacca gtccattatg atcgcccgta ttgcgcgcca
     6601 cgcagaagtt ttggttatta caggtgacct gccagtcgga aaacgcccgt tgcgctggtg
     6661 ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa gagaaggaaa atgcgatagc
     6721 gcatggacgg tacgacccca gaagttcaaa caaacagcac aagtcgtatc ttcgttgcgc
     6781 aggtagcgcg gctcaatcag atttgtcgga ttgagccaat aataaacaac gaaatattat
     6841 tgttattttt cagtccatta aatgtgacgt ttgcaaatag ttaagcaata acaccgtctt
     6901 accgtcacgt atctcgccgg ttttgatcat ctccagcgcc tggctgaacg gcagctcgag
     6961 cacttcaata tcttcatctt caacaccgcc accggcgtta gcgcgctgat tgtcactgta
     7021 ttcggcgata aaaaagtgga ttagctcagt cacaccgcca ggcgacatat acagttcaaa
     7081 taatttgcgc acttcgccca cttcatagcc cgtctcttca atcgcttctt tacgaatgca
     7141 cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagct gcccgctttc
     7201 attgccatta acccaggtag cgacacggaa ctgacgaatc agaaccacgc tctttttctt
     7261 cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg taaacttcac gtttatggcg
     7321 gataacttcg ccatctttgc gcgtgagatc gtaggtaatg ttgtgcaggg tgaaatagtt
     7381 atcggagaga attttgtctt taatgagggt gatttgttgc gtcataccga ctccacagcg
     7441 cgaaatgaac aattatctta cgctgtgaag tcgggtttgt ctgcgcaggc tatcagtgag
     7501 atttgactga ttttaccccc agccagtcaa taatcccctg tgccgcatga cgtccttctg
     7561 ccattgcggt aaccaccaga tccgcaccac gcacggcgtc gccaccggcg aagattttcg
     7621 gattggtggt ctggtaacgg tactggcttt ccacatccgc gatgatgcgg ccccatttgt
     7681 ctaccgttac accgtgcgac tccagccacg gcatcccgtg tggattgaag ccaaacgcca
     7741 taatcaccgc atcggctggc atgacaaatt cactgccctc aatcggtaca ggtcgccgac
     7801 gcccctgggc atccggctcg ccaagacgcg tgcgcaggaa acgaatcccg cagacgtgac
     7861 cttgttcatt cagctcaagc gccaccggct ggacgttaaa ttcgaagttg gccccctctt
     7921 cgcgggcgtt cctcacctct ttcttcgagc ctggcatgtt agcttcatca cgacgataag
     7981 cgcaggtgac gttactcgcg ccgtggcgca gcgcagtacg cacacagtcc atcgcggtgt
     8041 cgccgccgcc cagtaccacg acgttaagtc cggcggtatt gataaacggc tcttccggta
     8101 gctcttcgag ccccatcacc tgtttagtgt tggcaatgag gaacggcagc gcgtcataaa
     8161 cgcccggcgc atcttcattg ggtaaacccg cgttcatgga acggtaagtg cctacgccaa
     8221 cgaagaccgc gtcgtattgt tccaaaagcg aatccaaaga gacatcttta cccacttcac
     8281 aattgagttc gaagtgaatc cccatcgcgc tgaagatttc ccgacggcgt gccagcaggg
     8341 atttatccag tttgaaagaa ggaatgccga aagtgagcaa gccaccgatt tctggatggc
     8401 gatcgtacac cgtcaccgcc acgccattgc gggtcagaac atccgcacag gccagccctg
     8461 ccggacctgc cccgataatc gccacccgct tgtccacttt ggtgacatga cttaagtcag
     8521 gacgccaacc tttcgccaac gcctgatctg aaatatagcg ttcaatattg ccgatagtta
     8581 ccgcgccgtg ctcatcgcga atagtacagg caccttcaca caaacggtct tgcggacaaa
     8641 cgcgtccggt aatttccggc agggtgttgg tctggtgaga aagctcgacg gcggcgtcga
     8701 tgtttccggc tttcaccagt tcaatccact gcggaatatg gttatgcagc gggcaggtcc
     8761 attcacaaac gctgtgctcg ccgcacttaa ggcagcgcga ggcttcccgt tgtgcctggt
     8821 cggcgcgaaa tggcagataa atttcatcaa aaccggtttt gcgcgcttca atcgccagtt
     8881 tatccggctc gccacgcgcg ggcgttgcct gcatttgttc gactttactc attaccggca
     8941 tttcttgcgc cgcggtactg gcatgccacg gttgatgttc ctgacgcgcg gtgcgcaagc
     9001 ggcggggttt cgccatgccg gagagtgcga cgtcagtgac cagttgcagc gcgtccgccg
     9061 ggcaattctc aacacaggca ggaccgtttt cgcgccccgc acaaaggtcg catttatgcg
     9121 ccgtggcttt tacttttcct gccgcgacgg gtgtcaggac gatttgcatc gtaccaaaag
     9181 gacaggccac cacgcaggat ttacagccaa tacacttttg ctgattgacc tgaatgctgt
     9241 catcaacgtg gctgattgcg ccattagggc agctacgggc gcagggcgca tcttcacaat
     9301 ggtgacaggt cactgcacta cgttgctgtt gatgtttgat aaccgtaatt cggggatgaa
     9361 aatggtgttg gctcaggaca tgttgctcat cattgtgagc catgacacag gcgatttcac
     9421 aagcatgaca acccagacat tgctgactgt tggccataat aaaacgattc ataacgacct
     9481 tcttttttgg ttgtaaaaac cttattcttt atatgagtgt tgttattacc cgacttacag
     9541 gggaatcggc aatgttcatg tgcccagaat aagtaactat ttcgctataa actgtggcag
     9601 atcaaataat cccatcactg actaaattgt gtttcagcta actggaacat taatgatttt
     9661 ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc ggccagtctg gcccgggcct
     9721 ttttttacat tgtgctgctg tcgattcttt ccacgggtat cgctctgcta actctggcga
     9781 gcagtttgcg cgacgctgag gctatcaata ttgccggatc gctacgtatg cagagttacc
     9841 gcctgggcta cgacttgcaa agtggcagtc cacaactcaa tgcacatcgc cagttgtttc
     9901 agcaggcact gcattcaccg gtattaacca atctcaacgt ctggtatgtg ccagaagcag
     9961 taaaaactcg ctatgcgcat ctgaatgcca actggctgga gatgaataat cggctcagca
    10021 agggcgattt gccgtggtat caggccaata ttaataatta tgttaatcag atagacctgt
    10081 tcgtgctggc tttacagcac tacgctgaac gcaaaatgct gctggtggtg gcgatttccc
    10141 tggctggcgg tatcggtatt ttcacgctgg tcttttttac tttgcgccgt atacgccatc
    10201 aggtggttgc cccgctgaat cagctggtta ccgccagtca acgtattgaa cacgggcagt
    10261 tcgactcgcc gccgctggat accagtctgc cgaatgagct tggtctgctt gcaaaaacct
    10321 ttaaccagat gtcgagcgag ctgcataaat tgtacctttc gctggaggcg tcagtagaag
    10381 aaaagacccg cgatctccac gaggccaagc gtcgtctgga ggtgttgtat caatgttccc
    10441 aggcgctgaa caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc
    10501 gcgacaatga agcggctgaa tatctggagt taaatgtcgg tgaaaactgg cggattagcg
    10561 aagggcaacc aaacccggaa ttgccgatgc agattttacc ggtgacaatg caagagacgg
    10621 tttacggcga actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca
    10681 gtgtttcatc gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc
    10741 agttattgtt gatggaagaa cgtgcaacca tcgcccgcga attgcacgac tcgctggctc
    10801 aggtactttc ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata
    10861 acgccaccgc acaaagtatc atggccgatt tttcccaggc gttgaatgat gcttatcggc
    10921 agttacgcga gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg
    10981 cgttgaggga aatgctggat acgttacaaa atcaaaccag cgccaaactg accctcgact
    11041 gccgtctgcc aaccctggcg ctggatgcgc aaatgcaggt gcatttgttg caaattattc
    11101 gcgaagcggt gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg
    11161 tcaccgcgcc ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg
    11221 aaccgaaaga acccgaaggt cattatggtc tgaatatcat gcgcgaacgc gcggaacggc
    11281 tgggtgggac gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct
    11341 ttcgctctgc ggagggtgag gaaagtcagt tgatgtaatg cctcctactg accaaagaat
    11401 acttgcactt aaggttcagt ataaaagggc atgataattt acattaactc ctttttttct
    11461 ccacgattgg ctcgtacctt gccgctacag tgaagcaagt caagcctaca acgatacgca
    11521 gaaacacgag gtcctctttt aatggcgaat ttctttattg atcgccccat ttttgcctgg
    11581 gtgctggcaa tcctgttgtg tctgacaggg accctggcga ttttttcatt gcccgttgaa
    11641 caataccccg atctcgcgcc accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg
    11701 gcccagacgc tggaaaacac cgtgacccag gttatcgagc aaaatatgac cggcctcgat
    11761 aatctcatgt atatgtcatc tcagagcagt ggcaccggtc aggcatctgt cactttaagt
    11821 tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca
    11881 gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc
    11941 gataccaata ttctgaccat tgctttcgtc tctaccgatg gttcgatgga taaacaggat
    12001 attgccgatt atgttgccag taatattcag gacccgttaa gccgcgtgaa tggcgtcggg
    12061 gatatcgatg cctatggttc gcaatattcc atgcgtatct ggctggaccc ggcgaaactc
    12121 aacagtttcc agatgacggc taaagatgtc actgatgcca ttgagtcaca gaacgcgcag
    12181 attgcggttg ggcaacttgg tggtacacct tccgtcgata agcaggcgct caacgccacc
    12241 attaacgccc agtcactgct gcaaacacca gaacagttcc gcgatatcac cttgcgggtg
    12301 aatcaagacg gctcagaggt aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag
    12361 aaatacgatt atcttagccg cttcaatggt aagccagcct ccgggctggg ggtaaaactg
    12421 gcctccggcg caaacgaaat ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg
    12481 gcgcagtact tcccgcacgg actggaatac aaggtggcgt atgaaaccac ctcgtttgtt
    12541 aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag ctatcgccct ggttttcctc
    12601 gttatgtatc tgttcctgca aaacttccgc gccacgctga tacccactat cgccgtgccg
    12661 gtggtgttga tgggaacctt ctccgtactt tacgccttcg gttacagcgt caacacctta
    12721 accatgttcg cgatggtact ggcgatcggt ctgctggtgg atgacgcaat cgtggtggtg
    12781 gaaaacgtcg aacgtattat gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa
    12841 tcgatggggc agatccaggg ggcactggtc gggattgcga tggttctttc ggcggtattt
    12901 gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct atcgccagtt ctctattacc
    12961 attgttgcgg cgatggtgct gtcagtactg gtagcgatga tcctcactcc ggctctgtgt
    13021 gccacactac ttaagccact gaaaaaaggt gagcatcacg ggcaaaaagg cttttttgcc
    13081 tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt
    13141 ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc tgcttggcgg catggtgttc
    13201 ctgttcctgc gtttgccgac gtcgttctta ccgctggaag accgtggcat gtttactacc
    13261 tcggtacaat tgcccagcgg ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc
    13321 gagaaatatt acttcaccca tgaaaaagac aacatcatgt cggtgtttgc cactgttggt
    13381 tctggccccg ggggtaacgg gcaaaacgtg gcgcgaatgt ttatccgcct gaaagactgg
    13441 agcgaacgcg acagtaagac cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg
    13501 tttaaccaaa ttaaagaagc tcgcgttatc gccagcagcc cgccagcaat tagcggtctt
    13561 ggtagttctg caggttttga tatggagttg caggaccacg ctggagcggg tcacgatgcg
    13621 ctgatggcag cacgtaatca gttgctggcg ctggcggcgg aaaacccgga gctaacccgt
    13681 gtgcgccata acggcctcga cgacagtccg cagttgcaga ttgatatcga ccagcgtaaa
    13741 gctcaggcgc tgggcgttgc tatcgacgat attaacgaca cactgcaaac cgcctggggt
    13801 tcgagctatg tgaatgactt tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca
    13861 gctgcgccgt atcgcatgct gccagatgac attaatctct ggtatgtccg aaataaagat
    13921 ggcggcatgg tgcccttctc tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt
    13981 ctggaacgct ataacggtta ttctgcggtt gagattgttg gggaagccgc accgggggtc
    14041 agtaccggta cggcgatgga tattatggaa tcgttagtga agcagttgcc aaacggcttt
    14101 ggtctggagt ggacggcgat gtcgtatcag gagcgacttt ccggcgcgca ggctccggcg
    14161 ctgtacgcca tttccttgct ggtggtattc ctgtgtctgg cggcgttgta tgaaagctgg
    14221 tcggtgccgt tctcggtaat gctggtcgtg ccgctggggg ttatcggcgc gctgctggca
    14281 acctggatgc gcgggctgga aaacgacgtt tacttccagg tgggcctgtt aacggtcatt
    14341 ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa
    14401 ggccacgacc tgtttgaagc gacgctccac gcctgccgtc agcgtttacg cccgattctg
    14461 atgacctcgc tggcatttat cttcggcgta ttgccaatgg caaccagcac gggtgccggt
    14521 tccggtggtc agcatgcggt gggtactggc gtaatgggcg ggatgatttc agctactatt
    14581 ctggctattt acttcgtgcc gctgttcttt gtgctggtgc gccgccgctt cccgctgaag
    14641 ccgcgcccgg aataagcaat aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt
    14701 ccgattaaga acctgctcag cgggttcttg ctgtttatac tttgtctcgg gaattactta
    14761 cgaagcataa cttcgataaa gtctttccag ttccccagtt cacgttcaat cataacaacc
    14821 tctcttataa ttatgggtat tctacggaaa caatataccg tggtgaagct aatttactcg
    14881 attgctgcga tgactacctc cgggggacaa accttatgta aatactatgg tcctgcggtg
    14941 ataatttgta tgtgatacac agcaacattt cgagat
//
LOCUS       DAZGOB010000127        14944 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGOB010000127 DAZGOB010000000
VERSION     DAZGOB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.127"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(87..1337)
                     /gene="icd"
                     /locus_tag="ACHLIF_004015"
     CDS             complement(87..1337)
                     /gene="icd"
                     /locus_tag="ACHLIF_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO9607928.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1509..2162
                     /gene="rluE"
                     /locus_tag="ACHLIF_004016"
     CDS             1509..2162
                     /gene="rluE"
                     /locus_tag="ACHLIF_004016"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO9607929.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2172..2633
                     /gene="nudJ"
                     /locus_tag="ACHLIF_004017"
     CDS             2172..2633
                     /gene="nudJ"
                     /locus_tag="ACHLIF_004017"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO9607930.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2687..3793
                     /gene="mnmA"
                     /locus_tag="ACHLIF_004018"
                     /gene_synonym="trmU"
     CDS             2687..3793
                     /gene="mnmA"
                     /locus_tag="ACHLIF_004018"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO9607931.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3829..4470
                     /gene="hflD"
                     /locus_tag="ACHLIF_004019"
     CDS             3829..4470
                     /gene="hflD"
                     /locus_tag="ACHLIF_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO9607932.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4474..5844
                     /gene="purB"
                     /locus_tag="ACHLIF_004020"
     CDS             4474..5844
                     /gene="purB"
                     /locus_tag="ACHLIF_004020"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO9607933.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6012..6683
                     /gene="phoP"
                     /locus_tag="ACHLIF_004021"
     CDS             6012..6683
                     /gene="phoP"
                     /locus_tag="ACHLIF_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO9607934.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6683..8143
                     /gene="phoQ"
                     /locus_tag="ACHLIF_004022"
     CDS             6683..8143
                     /gene="phoQ"
                     /locus_tag="ACHLIF_004022"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO9607935.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8219..9340
                     /gene="roxA"
                     /locus_tag="ACHLIF_004023"
                     /gene_synonym="ycfD"
     CDS             8219..9340
                     /gene="roxA"
                     /locus_tag="ACHLIF_004023"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO9607936.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9486..10715)
                     /gene="pepT"
                     /locus_tag="ACHLIF_004024"
     CDS             complement(9486..10715)
                     /gene="pepT"
                     /locus_tag="ACHLIF_004024"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO9607937.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10965..12101
                     /gene="potA"
                     /locus_tag="ACHLIF_004025"
     CDS             10965..12101
                     /gene="potA"
                     /locus_tag="ACHLIF_004025"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO9607938.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12085..12948
                     /gene="potB"
                     /locus_tag="ACHLIF_004026"
     CDS             12085..12948
                     /gene="potB"
                     /locus_tag="ACHLIF_004026"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO9607939.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVNELDD"
     gene            complement(13181..13330)
                     /locus_tag="ACHLIF_004027"
                     /pseudo
     CDS             complement(13181..13330)
                     /locus_tag="ACHLIF_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            13454..13539
                     /locus_tag="ACHLIF_004028"
                     /pseudo
     CDS             13454..13539
                     /locus_tag="ACHLIF_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(13562..14152)
                     /gene="espM2"
                     /locus_tag="ACHLIF_004029"
     CDS             complement(13562..14152)
                     /gene="espM2"
                     /locus_tag="ACHLIF_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFO9607940.1"
                     /translation="MPMNTSGTSFSSFGISCHREGSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSRNILDTVNRQSAKDIDGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPAYLTRAIQG"
     gene            14336..>14944
                     /locus_tag="ACHLIF_004030"
     CDS             14336..>14944
                     /locus_tag="ACHLIF_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO9607941.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCF"
BASE COUNT         3714 a         3734 c         3658 g         3838 t
ORIGIN      
        1 atgctcgata tgctattaat aatacctaca taaataaaac aacgggcgtg ttatacgccc
       61 gttgcaatat taaacacatg tagtgattac atgttcttga tgatcgcatc accaaattct
      121 gaacatttca gcagtttagc gccttccatc agacgctcga agtcataggt tacggtcttc
      181 gcattgattg cgccttccat acctttaaca atcaggtctg cggcttcagt ccagcccata
      241 tggcgtaaca tcatctcagc ggagagaata atagagccag ggtttacttt gtcctgaccg
      301 gcgtatttcg gcgcagtacc gtgggtggct tcaaacaggg cgcattcgtc accgatgttt
      361 gctccagggg cgataccgat accgccaacc tgcgctgcca gagcgtcaga aatgtagtca
      421 ccgttcaggt tcatacaggc gataacatca tattcagccg gacgcagcag gatctgttgc
      481 aggaatgcat cagcaatcac gtctttaatg acgatctctt tgccggtgtt cgggttttta
      541 actttcagcc acgggccgcc gtcgatcagt tcaccgccaa actcttcacg cgccagctgg
      601 tagccccagt ctttaaacgc gccttcggtg aacttcatga tgttgccttt gtgcaccaga
      661 gtcacagagt cacgatcgtt agcaattgcg tattcgatcg ctgcacgaac cagacgtttg
      721 gtgccttctt ccgaacacgg cttaataccg ataccacaat gttccgggaa gcgaattttc
      781 ttcaccccca tctcttcacg caggaattta atcactttct cggcgtcggc agagtctgct
      841 ttccattcga tacccgcata aatgtcttcc gagttttcac ggaagataac catatcggtc
      901 agttcagggt gtttaaccgg gcttggagtg ccttgatagt aacgtaccgg acgcaggcag
      961 atgtagagat ccagttcctg gcgcagggca acgttcagag agcgaatacc gccaccaaca
     1021 ggagtggtca gcggaccttt aatggcaacg cgatattcac gaatcagatc aagagtttca
     1081 gcaggcagcc agacgtcctg accataaacc tgtgtggatt tttcaccggt gtaaatttcc
     1141 atccaggaga ttttacgctc gcctttatag gctttctcga ctgcagcgtc gaccactttc
     1201 agcatggctg gggttacatc tacaccgatt ccatcacctt caatgtaagg gataatcgga
     1261 ttttcaggaa cgttgagttt gccgttttgc agggtgatct tcttgccttg tgccggaaca
     1321 actactttac tttccattca cctctccttc gagcgctact ggtttgctcg tctgccacca
     1381 cgttgcatat gcgtttgcgt cctgcgatac ggatgcttta gagcaatttt ttgttaatga
     1441 tttgtaattg gcttgtcaat actaccctat tgtttgccgt catgaaagat gcgcgttatt
     1501 aggctataat gcggcaattc ataatctctg aaaataccat gcaaaaaact tcttttagaa
     1561 atcaccaagt taagcgattc agctcgcaac gttctaccag gcgtaaacct gaaaaccagc
     1621 ccacgcgtgt gatcctgttc aataaaccct acgatgttct tccgcagttc accgatgaag
     1681 ccggacgcaa aacattaaaa gaattcatcc cggttcaggg cgtttatgca gcaggtcgtc
     1741 ttgaccgcga tagcgaaggg ttactggtgc tgaccaataa cggcgcgttg caggcgcgtt
     1801 taacccagcc gggcaaacgc accgggaaaa tctattatgt gcaggtggaa ggtattccca
     1861 cacaagacgc acttgaagcc ttgcgcaatg gcgtaacctt aaatgatggc cctaccctgc
     1921 ccgcaggcgc ggagctggtt gacgaacccg cgtggttatg gccgcggaat ccaccaattc
     1981 gtgaacgcaa aagtattccc accagctggc tgaagatcac cttatatgaa ggacgtaatc
     2041 gccaggtgcg ccgcatgacc gcccatgttg gtttccccac gctgcgactg attcgctatg
     2101 cgatgggaga ttactctttg gataatcttg ccaatggcga atggcgagaa gtgacagatt
     2161 aaggatgcaa aatgtttaaa ccgcacgtta ccgttgcttg cgtggtgcac gcagaaggca
     2221 aatttttagt cgttgaagag acgattaatg gtaaagcgtt atggaaccaa cctgccgggc
     2281 atctggaagc cgatgaaacc ttagtggaag ccgccgcccg tgaactgtgg gaagaaaccg
     2341 gcatcagcgc gcagccgcaa cactttattc gtatgcatca gtggattgcg ccagataaaa
     2401 cgccgttttt gcgtttcctc tttgccattg agcttgagca aatatgcccg acccagcctc
     2461 atgacagcga tatcgactgc tgtcgttggg tcagcgccga agaaatttta caggcgtcaa
     2521 atcttcgttc gccgctggtg gcggaaagta tccgttgtta tcaaagcggg caacgctatc
     2581 cgctggagat gattggcgat tttaactggc cttttacaaa gggtgtcatc taaaaggctg
     2641 cgtgatagaa tacgccgcct tgaagttcaa tgtcgtgagt gatccaatgt ctgaaaccgc
     2701 aaaaaaagta atcgtcggca tgtccggcgg tgtcgattcc tccgtttctg cctggctgtt
     2761 gcaacaacag ggatatcagg tcgaaggcct gtttatgaag aactgggaag aagacgacgg
     2821 tgaggaatat tgcacagcgg cagcggatct ggctgatgcc caggctgtct gcgacaagct
     2881 cggcattgaa ctgcacaccg ttaactttgc tgccgagtac tgggacaacg tcttcgaact
     2941 gttccttgcc gaatataaag ccggtcgcac gccgaatccg gatattctgt gcaacaaaga
     3001 gatcaaattt aaagccttcc tcgaatttgc cgccgaagat ttaggtgccg attatatcgc
     3061 taccggtcat tacgtacgtc gggccgatgt cgatggcaag agccgcctgc tgcgtggtct
     3121 ggacagcaat aaagaccaga gctacttcct ttatacgctc agccatgagc agattgcgca
     3181 aagcctgttc ccggtcggcg aactggaaaa accgcaggtg cgtaagattg ctgaagatct
     3241 tggtctggtc accgcgaaga aaaaagactc taccggcatt tgcttcattg gcgaacgtaa
     3301 attccgcgag ttcctgggcc gttatctccc ggcgcaaccg ggcaaaatca ttaccgtcga
     3361 tggcgatgaa attggtgagc accaggggct gatgtatcac actctcggtc agcgtaaagg
     3421 tctgggtatc ggtggcacca aagaaggtac cgaagaaccg tggtatgtgg tggacaaaga
     3481 cgtcgaaaac aacattctgg ttgtcgctca gggccatgaa cacccgcggc tgatgtctgt
     3541 cgggttgatt gcccagcagt tgcactgggt cgatcgcgaa ccattcaccg gcactatgcg
     3601 ttgcacggta aaaacccgct atcgccagac cgatatccct tgcaccgtca aggcgctgga
     3661 cgatgatcgc attgaagtga ttttcgatga accggttgcc gccgtgacgc cgggccagtc
     3721 tgccgtcttc tataacggtg aagtgtgcct cggtggcggt attattgagc agcgtctgcc
     3781 gctgccggtc tgattattat ctttacttaa aacaaggaag cagtgaacgt ggcaaagaat
     3841 tactatgaca tcaccctcgc cctggccggt atttgtcagt cggcacgcct ggtgcaacaa
     3901 ctcgctcacc aggggcattg tgatgccgat gcgctacacg tctcactcaa cagtattatt
     3961 gatatgaacc ccagctcgac gctggcggtt tttggcggta gcgaagccaa cctgcgcgtc
     4021 gggctggaaa ccctgctcgg cgtgctcaat gccagcagtc gccagggctt aaacgccgaa
     4081 ttaacccgct acacactcag cttgatggtg cttgagcgca aactctcctc agcgaaaggc
     4141 gcgctcgaca ctctgggcaa ccggatcaac ggcctgcaac gccagctcga acacttcgat
     4201 ttacagtccg aaacgctgat gagcgcgatg gctgctatct atgttgatgt gatcagcccg
     4261 cttggcccgc gcattcaggt caccggttcc cctgctgtac tgcaaagccc acaagtgcag
     4321 gcgaaagttc gcgcaaccct gctggcaggc attcgcgccg ccgtgctctg gcaccaggtc
     4381 ggcggcggac gtctgcaact gatgttttct cgtaatcgcc tgaccactca ggcaaaacaa
     4441 attcttgctc atttaacccc ggagttgtga tctatggaat tatcctcact gaccgccgtt
     4501 tcccctgtcg atggacgcta cggcgataaa gtcagcgcgc tgcgcgggat tttcagcgaa
     4561 tatggtttgc tgaaattccg tgtacaagtt gaagtacgtt ggctgcaaaa actggccgcg
     4621 cacgcagcga tcaaggaagt tcctgctttt gctgccgacg caatcggtta ccttgatgca
     4681 attgtcgcca atttcagcga agaagatgcc gcacgcatca aaaccatcga gcgtaccact
     4741 aaccacgacg ttaaagcggt tgagtatttc ctgaaagaaa aagtggcgga gatcccggaa
     4801 ctgcacgcgg tttctgaatt catccacttt gcctgtactt cggaagatat caataacctc
     4861 tcccacgcat taatgctgaa aaccgcgcgt gatgaagtga tcctgccgta ctggcgtcaa
     4921 ctgattgatg gcattaaaga tctcgccgtt cagtaccgcg atatcccgct gctgtctcgt
     4981 acccacggtc agccagccac gccgtcaacc atcggtaaag agatggcaaa cgtcgcctac
     5041 cgtatggagc gccagtaccg ccagcttaat caggtggaga tcctcggcaa aatcaacggc
     5101 gcggtcggta actataacgc ccatatcgcc gcttacccgg aagttgactg gcatcagttc
     5161 agcgaagagt tcgtcacctc gctgggtatt cagtggaacc cgtacaccac ccagatcgaa
     5221 ccgcacgact acattgccga attgtttgat tgcgttgcgc gcttcaacac cattctgatc
     5281 gactttgacc gtgacgtctg gggttatatc gcccttaacc acttcaaaca gaaaaccatt
     5341 gctggtgaga ttggttcttc caccatgccg cataaagtta acccgattga cttcgaaaac
     5401 tccgaaggga atctgggcct ttccaacgcg gtattgcagc atctggcaag caaactgccg
     5461 gtttcccgct ggcagcgtga cctgaccgac tccaccgtgc tgcgtaacct cggcgtgggt
     5521 atcggttatg cgctgattgc gtatcaatcc accctgaaag gcgtgagcaa actggaagta
     5581 aaccgtgacc atctgctgga tgagctggat cacaactggg aagtgctggc tgagccaatc
     5641 cagacagtta tgcgtcgcta tggcatcgaa aaaccgtacg agaagctgaa agagctgact
     5701 cgcggtaagc gcgttgacgc cgaaggcatg aagcagttta tcgatggtct ggcgttgcca
     5761 gaagaagaga aagcccgcct gaaagcgatg acgccggcta actatattgg tcgagctatc
     5821 accatggttg atgagctgaa ataaactcgt atcagtgccg gatggcgatg ctgtccggcc
     5881 tgtttattaa aattatccac tttttctttt ttcactttac ctcccctccc cgctggttta
     5941 tttaatgttt acccccataa acacataatc gcgttacact attttaataa ttaagacagg
     6001 gagaaataaa aatgcgcgta ctggttgttg aagacaatgc gttgttacgt caccacctta
     6061 aagttcagat tcaggatgct ggtcatcagg tcgatgacgc agaagatgcc aaagaagccg
     6121 attattatct caatgaacat ttaccggata ttgcgattgt cgatctcgga ttgcctgacg
     6181 aggacggtct gtcactgatt cgccgctggc gtagcaatga tgtttcactg ccgattctgg
     6241 tattaaccgc ccgtgaaagc tggcaggaca aagtcgaagt attaagtgcc ggtgctgatg
     6301 attacgtgac taaaccgttt catattgaag aggtgatggc gcgaatgcag gcattaatgc
     6361 ggcgtaatag cggcctggct tcacaggtca tttcgctacc cccgtttcag gttgatctct
     6421 ctcgccgtga attatctatt aatgacgaag tgatcaaact gaccgcgttc gaatacacca
     6481 ttatggaaac gttgatacgc aataatggca aagtggtgag caaagattcg ttaatgctcc
     6541 aactctatcc ggatgcggag ctgcgggaaa gccataccat tgatgtactg atgggacgtc
     6601 tgcgcaaaaa aattcaggca caatatccgc aagaagtgat tactaccgtt cgcggccagg
     6661 gctatttgtt cgaattgcgc tgatgaaaaa attactgcgt ctttttttcc cgctctcact
     6721 gcgggtacgt tttctgttgg caacggctgc ggtagtactg gtgctttcgc ttgcctacgg
     6781 aatggtcgcg ctgatcggtt atagcgtcag tttcgataaa actacgtttc ggctgttacg
     6841 tggcgagagc aatctgttct atacccttgc gaagtgggaa aacaataagt tgcatgtcga
     6901 gttacccgaa aatatcgaca agcaaagccc caccatgacg ctaatttatg atgagaacgg
     6961 gcagctttta tgggcgcaac gtgacgtgcc ctggctgatg aagatgatcc agcctgactg
     7021 gctgaaatcg aatggttttc atgaaattga agcggatgtt aacgatacca gcctcttgct
     7081 gagtggagat cattcgatac agcaacagtt gcaggaagtg cgggaagatg atgacgacgc
     7141 ggagatgacc cactcggtgg cagtaaacgt ctacccggca acatcgcgga tgccaaaatt
     7201 aaccattgtg gtggtggata ccattccggt ggagctaaaa agttcctata tggtctggag
     7261 ctggtttatc tatgtgctct cagccaatct gctgttagtg atcccgctgc tgtgggtcgc
     7321 cgcctggtgg agtttacgcc ccatcgaagc cctggcaaaa gaagtccgcg aactggaaga
     7381 acataaccgc gaattgctca atccagccac aacgcgagaa ctgaccagtc tggtacgaaa
     7441 cctgaaccga ttgttaaaaa gtgaacgcga acgttacgac aaataccgta cgacgctcac
     7501 cgacctgacc catagtctga aaacgccact ggcggtgctg caaagtacgc tgcgttctct
     7561 gcgtagtgaa aagatgagcg tcagtgatgc tgagccggta atgctggagc aaatcagccg
     7621 catttcacag caaattggct actacctgca tcgtgccagt atgcgcggcg ggacattgct
     7681 cagccgcgag ctgcatccgg tcgccccact gctggacaat ctcacctcag cgctgaacaa
     7741 agtgtatcaa cgcaaagggg tcaatatctc tctcgatatt tcgccagaga tcagctttgt
     7801 cggtgagcag aacgattttg tcgaggtgat gggcaacgtg ctggataatg cctgtaaata
     7861 ttgcctcgag tttgtcgaaa tttctgcaag gcaaaccgac gagcatctct atattgtggt
     7921 cgaggatgat ggccccggta ttccattaag caagcgagag gtcattttcg accgtggtca
     7981 acgggttgat actttacgcc ctgggcaagg tgtagggctg gcggtagccc gcgaaatcac
     8041 cgagcaatat gagggtaaaa tcgtcgccgg agagagcatg ctgggcggtg cgcggatgga
     8101 ggtgattttt ggtcgccagc attctgcgcc gaaagatgaa taaatatgtc catacttcac
     8161 gcattacgtt aagcatccgt tataatcggt tgcagatacc agcctgtgga tgcttaacat
     8221 ggaataccaa ctcactctta actggcccga ttttcttgaa cgtcactggc agaaacgccc
     8281 ggtggtgtta aaacgcggct ttaataattt tattgacccg atctctccag acgagttggc
     8341 gggtctggcg atggaaagcg aagttgacag tcgactggtc agtcaccagg atggcaaatg
     8401 gcaggtcagc cacggcccgt tcgaaagcta cgatcatctc ggtgaaacca actggtcatt
     8461 actggtacag gcagtgaacc actggcatga gccgaccgcc gcgctgatgc gaccgttccg
     8521 tgaactaccg gactggcgta ttgatgatct gatgatctct ttttctgtac cgggcggcgg
     8581 cgtcggcccg catctcgatc agtacgacgt gtttatcatt cagggtaccg gtcgtcgtcg
     8641 ctggcgagtg ggcgaaaagc tgcaaatgaa acagcactgt ccgcacccgg atctgttaca
     8701 ggtcgatccg ttcgaagcca tcatcgatga agagctggag cctggcgata ttctttatat
     8761 tccgccagga ttcccgcatg aaggctacgc gctggaaaat gcgatgaact attccgtggg
     8821 ttttcgcgcg ccaaatacgc gggaattaat tagcggattt gccgattatg tgctgcaacg
     8881 tgaactgggc ggcaactact acagcgatcc ggatgttcca cctcgcgctc atcctgcgga
     8941 cgttctgccg caagagatgg ataaactgcg tgagatgatg ctcgaattga tcaaccagcc
     9001 ggaacacttt aagcaatggt ttggcgagtt tatatcccag tcacgtcatg aactggatat
     9061 cgcgccgccg gaaccgcctt atcagccaga tgaaatctac gatgcgctga aacaaggtga
     9121 agtgctggtg cgcctgggtg gtctgcgcgt attgcgcatt ggcgacgacg tgtatgccaa
     9181 tggtgagaag atcgattccc cgcaccgtcc ggcactggat gcactcgcca gcaacattgc
     9241 gctgactgcg gagaattttg gcgatgcgct ggaagatccg tcattcctcg cgatgctcgc
     9301 ggcgctggtc aatagcgggt attggttctt cgaagggtaa gtttggattt aagccggatg
     9361 cggcgctgat tgcgccttat cgtgcctaca tgcactgaac gcctgtaggc acgataagta
     9421 tgtggaccag tatcaatata acggtttcat cccggtaatt ggcagtccac atacgcatcg
     9481 ggcaatcacg acttccgttg cgccgttaac tcggcaatac ggacaatcac ctgcaccgct
     9541 ttttccatac cttccagagt cacaaactca tgcttaccat gatagttgta accgccagtg
     9601 aacaggttcg ggcacggtaa tcccataaac gacaactgcg cgccgtcggt accgccgcgg
     9661 atcggtttca gttccggttc aatatcgcag tcacgcatcg cctgctgggc gatatcgata
     9721 atatgcggat gctcaaccac tttctcgcgc atattgtagt aactgtcttc aatcaccagt
     9781 tcaatgtagc aatcaggatg taaccctttg cccacttttt tggcgatctc catcatttta
     9841 cgtttacgcg cttcaaactg tttacggtcg aaatcacgga tgatgtagtg catatcggct
     9901 cggtcaacgg tgcctttcat gctcgccagg tgatagaaac cttcatagcc ttctgtcatt
     9961 tccgggcttt catccgccgg aacttccgca tgaatacgtg ccgccagcga cagcgcattt
    10021 accatcactc ctttcgccgt gcccggatga acattgttac cgacaatttt gatattgacc
    10081 gatgcggcgt tgaagttttc aaactccagt tcgcctacgc caccaccgtc aacagtgtaa
    10141 gcccagcggg catcgaaggc atcaacatca aaatgtttcg cccctttgcc cacttcttca
    10201 tccggggtaa aggcgacgcg aatatcacca tgcggaatgt ttttctgttg caataccgcc
    10261 agcgcggtca tgatttctgc aatacctgct ttgtcatcgg cacctaacaa ggttttacca
    10321 tcggtggtaa tcaacgtctg acccagtagc tgatgcagca ccgggaacat aaccggtgat
    10381 aaaacttcat cgccgatacc cagcgcaata tcgccaccgc gatagttttc aacaatttgc
    10441 ggattcacat ttttgccgct gcaatccggt gaggtatcca catgagaaat aaagccaatc
    10501 gccgggatat cgccagggac gttagccggt aacgtcgcca tcaaagtgcc cttctcactt
    10561 aaggtcacat tgataagccc catctcttcg agctgctctt tcagcagatg caataacttc
    10621 cattggcctt ccgtgctggg aacctgtctc acccctgctt ttgattgggt atccagagac
    10681 acgtagttca aaaatcgctc aagtagttta tccatgtagt caccctcact ttttgtgaca
    10741 acattattaa gaagcaagaa aagacaaata ttgcgtcagg tcacttttac ccctgcaagc
    10801 gggaatattt atcagcatta ccttaatgaa tataaagcta agccagtaat tcacaacaaa
    10861 gattggcgat tcaagcggtt tgccgtagaa ttaccgccct cattaagagt caccaaggtg
    10921 gttaaccaca aaccccgcat cggtaagcca tccgttgcgt ttacatggga cagagtaaaa
    10981 aattgaataa acaaccgagt tcgctttcac cgctggtgca attggcggga attcgcaaat
    11041 gctttgatgg taaagaggtc attccccagc tggatctgac tatcaacaat ggcgagttcc
    11101 tcacgctgct tggcccttct ggctgcggta aaacaaccgt tcttcgcctg attgcaggtc
    11161 tggaaactgt tgattccgga cgcatcatgc tggataacga ggacatcacc cacgttccgg
    11221 cggaaaaccg ctatgtgaac actgttttcc aaagctacgc acttttcccc cacatgaccg
    11281 tgttcgaaaa tgtggccttt gggttgcgca tgcaaaaaac ccccgctgct gaaattacgc
    11341 cccgcgtgat ggaagccctg cggatggtgc aactggaaac cttcgctcaa cgtaaaccgc
    11401 atcagctctc tggtggtcaa cagcaacgcg tcgccattgc tcgcgcggtg gttaacaagc
    11461 ctcgtctgtt gttgctggat gagtcgctct cagcgctgga ttacaaactg cgtaagcaaa
    11521 tgcagaacga gctgaaagcg ttacagcgta agcttggtat taccttcgtc tttgtcactc
    11581 acgatcagga agaagcgctc accatgtcag acaggattgt ggtgatgcgc gagggtcgta
    11641 ttgagcaaga tggtacaccg cgcgaaattt acgaagagcc gaaaaacctg tttgttgccg
    11701 gcttcattgg cgaaatcaat atgtttaacg ccactgtcat cgaacgtcta gacgagcagc
    11761 gcgtacgcgc caacgttgaa ggccgcgaat gtaatatcta cgttaacttc gccgttgaac
    11821 cggggcaaaa actgcatgtt ctgctgcgcc cggaagactt acgtgttgaa gagattaacg
    11881 acgacaacca cgctgaaggg ctgattggtt acgttcgtga gcgtaactac aaaggcatga
    11941 cgctggagtc ggttgttgaa ctggaaaatg gcaagatggt gatggtcagc gaattcttca
    12001 atgaagacga tcctgacttt gaccactctc tcgaccaaaa aatggccatt aattgggtag
    12061 aaagctggga ggtcgtactg gctgatgaag aacacaagta agttccagaa tgtagtgatt
    12121 gtcactattg tcggttggct tgtgttgttt gtctttctgc ccaacctgat gatcattggc
    12181 accagctttt tgacccgcga cgacgccagt ttcgtaaaaa tggtctttac gctggataac
    12241 tacacgcgtc tgctcgatcc gctctatttt gaagtgctat tgcactcgct gaatatggcg
    12301 ctgatcgcca ccctcgcctg cctggtgctg ggctacccgt ttgcctggtt tctggcgaag
    12361 ctgccacaca aggtgcgtcc gctgctgctg tttctgctga ttgttccctt ctggaccaac
    12421 tcattgattc gtatctacgg gctgaaaatt ttcctcagca ccaaaggcta tctcaacgag
    12481 tttttgctct ggctgggcgt tatcgacaca ccaatccgta tcatgttcac gcccagtgcg
    12541 gtgattatcg gtctggttta cattctgctg ccgtttatgg tgatgccgct gtactccagt
    12601 atcgaaaaac tggataagcc gttgcttgag gcggcgcgcg atctcggtgc cagcaagtta
    12661 cagactttta tccgtatcat tattccactg acgatgccgg gaattattgc cggatgtctg
    12721 ctggtgatgc tgccagcgat gggcctgttc tatgtatccg acctgatggg cggtgcgaaa
    12781 aacctgctga tcggtaacgt catcaaggta cagttcctca atattcgtga ctggccattt
    12841 ggtgcagcca ccagcattac gctgactatc gtaatgggcc tgatgttgct ggtttactgg
    12901 cgcgcttctc gtctgctgaa taagaaggtg aacgaattag atgattaatc ctttatattc
    12961 aatttattag gtttgttttt gagtgatttt gtcgtttaag gtaacggcgt tatagatcct
    13021 atcaaaatca atcaattgca ttctggtttt ggagaaagat ttttcgagag cttcggtctt
    13081 taactaaaac tgattgcgta taatcgcaat accccctccc cgactatctg aatggtcggg
    13141 gaggcacttc aagcacaaaa tatcatccct gctccgttga ttactctaca ggtgaagtcg
    13201 gccactcaat atcaggtgct gctgatgtat caacacgatt caacaacacc cgatactttt
    13261 tccaggcttc ccgcaatgaa gtctcttcct caattacgat ctccagatct gcagcatcct
    13321 gaagtggggc gcaccacgca aacatcacaa aatattgtcg ttgtgatgga cctgtgcaat
    13381 agtgaatcac gctacgtttc tgtgtacaac gtactgtagt tttctttacg atttatagct
    13441 gtactggtga gccatgagta atctgaatcc atgcatggcg tgtggtgcct gttgtgcatt
    13501 tttctgcgtc tcttttactg gtccgaagcc gatgatgctg aagcagtcct ctcctctagc
    13561 ctcatccctg tatagcacgc gtcaagtaag ctgggatatc atttcgtagt tttacaatat
    13621 caatatccga atcgatatgt ttctggtaaa acttgtccgc aagcaagttt gctatggtat
    13681 ctgatgtacg attttttaca tcccggctca taccatcaca aagagcatcc attttcttgc
    13741 caaaagaagc actccccatt attacatgat taatagaact ttgtgctgcc ttaacatcaa
    13801 gggttagttg attctcctgg ctcacaagag aaaagactct ttgcctctct tcagtggaaa
    13861 tttttgcatt tttttgaaaa cagtaattat caatctcctg gttgatcacc cttgagggat
    13921 atactatgcg ttcatctttt atccacccat caatatcttt agcactctgc ctgttcactg
    13981 tatccagtat atttctgcta aatttgcgaa cagaaaagct aatagtccgt tctccaattg
    14041 aacattttac gacctcatca ttcttccctc ggaaagagtt cctaaaggag ccttctctat
    14101 ggcaacttat accaaaagag ctgaaagacg tacctgaagt attcatcggc attataattc
    14161 ctttttataa aaaagcgttt acaagattgc atgcttgtgt ttattgtagc cagaaaaaac
    14221 atgcatgcaa ataatattct attaaaagtt gtattgtttg agtcccatct catgatcggt
    14281 atgttgactc ttgttaccaa tatagttcac aaaatgggtt gaaaaggata ataatatgcc
    14341 agtcatatta aatttttcta atggaagtgt attgccggaa aatgagctgg aggctttacg
    14401 acatattgca agaagcaatc agaatgacac tattactata ggaggccgta atatgaggct
    14461 tcattatatc cagtttatgg atggttttag tgtagagcct attcctggtg gactttggga
    14521 ccatcttggg gcaagagagt ctcatcatct cgcagacagc ctggcaagac aacttaacgg
    14581 aggtaacact tttctgcagg catacagctt atatctggag cagaggcaag ctgccccgct
    14641 tgtgcaggaa agcgtcataa aaacactact agatcgaata aattcgaatg catttcccgt
    14701 tagtttacaa gacttttcct gtactgagga gcatcttaat tgtccgataa cgctacatat
    14761 tcctgagaca ggtgtttttg tcagaaatgc tcgaaattca gaaatatgtg cattatatga
    14821 tcaggaagca ttgactgaac ttatcctgcg taacgctctc caccccctca gccgtgaccc
    14881 ctttgctcca gaaatgatta taagcaaaga caagtgtcat tttaatataa caaaacaatg
    14941 tttt
//
LOCUS       DAZGOB010000128        14822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGOB010000128 DAZGOB010000000
VERSION     DAZGOB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.128"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..176
                     /locus_tag="ACHLIF_004031"
     ncRNA           45..176
                     /ncRNA_class="other"
                     /locus_tag="ACHLIF_004031"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(360..1037)
                     /gene="narI"
                     /locus_tag="ACHLIF_004032"
     CDS             complement(360..1037)
                     /gene="narI"
                     /locus_tag="ACHLIF_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO9607942.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1037..1747)
                     /gene="narJ"
                     /locus_tag="ACHLIF_004033"
     CDS             complement(1037..1747)
                     /gene="narJ"
                     /locus_tag="ACHLIF_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO9607943.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1744..3282)
                     /gene="narH"
                     /locus_tag="ACHLIF_004034"
     CDS             complement(1744..3282)
                     /gene="narH"
                     /locus_tag="ACHLIF_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO9607944.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3279..7022)
                     /locus_tag="ACHLIF_004035"
     CDS             complement(3279..7022)
                     /locus_tag="ACHLIF_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO9607945.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7537..8928)
                     /gene="narK"
                     /locus_tag="ACHLIF_004036"
     CDS             complement(7537..8928)
                     /gene="narK"
                     /locus_tag="ACHLIF_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO9607946.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9267..11063
                     /gene="narX"
                     /locus_tag="ACHLIF_004037"
     CDS             9267..11063
                     /gene="narX"
                     /locus_tag="ACHLIF_004037"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO9607947.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            11056..11706
                     /gene="narL"
                     /locus_tag="ACHLIF_004038"
     CDS             11056..11706
                     /gene="narL"
                     /locus_tag="ACHLIF_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO9607948.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11707..13101)
                     /gene="ychO"
                     /locus_tag="ACHLIF_004039"
                     /gene_synonym="iatA"
     CDS             complement(11707..13101)
                     /gene="ychO"
                     /locus_tag="ACHLIF_004039"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO9607949.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            13286..13639
                     /gene="ychN"
                     /locus_tag="ACHLIF_004040"
     CDS             13286..13639
                     /gene="ychN"
                     /locus_tag="ACHLIF_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO9607950.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13683..14378)
                     /gene="chaC"
                     /locus_tag="ACHLIF_004041"
     CDS             complement(13683..14378)
                     /gene="chaC"
                     /locus_tag="ACHLIF_004041"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO9607951.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14536..14766)
                     /gene="chaB"
                     /locus_tag="ACHLIF_004042"
     CDS             complement(14536..14766)
                     /gene="chaB"
                     /locus_tag="ACHLIF_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO9607952.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
BASE COUNT         3589 a         4030 c         3744 g         3459 t
ORIGIN      
        1 cttttcaaaa gtccgtaaac tctcaaacga atccgcaatt aaatattctg cccaagcggg
       61 gaaggatgag aagcttcgac caaggttcga cttgagcgcc agcgagagag cgttgccgca
      121 ggcaacgacc cgaagggcga agcgcgcagc gttgagtaat ccttccccca ccaccatcac
      181 ttccttaaat aatcaaaacc tgttactccg agctaatttt gcccgatggc accacgttta
      241 ccgggcctac aatatcctcc cctgtaggtc ggataagcga agcgcatccg acaactcacc
      301 ccgcaccgtg cccataaaaa aaccccgccg aagcagggtc tatgtgaact aaaattcgct
      361 tagtgacgag cgcgcaccag ctggtactta cgtgtcagat actccaccgg tacgctccag
      421 atgtgtacca gacgcgagaa cgggaacagc aggaataacg tcatccccag caccaggtgc
      481 agacggaaga taaacgccac accatcgagg tgttgagaag cgccaccgtg gaaggtcacc
      541 accgactgcg cccagccaac cagtttcatc atctcgctac cgtccatatg ctgagcggag
      601 aacggaatgg tcaacaggcc cagcgcgcac tggataacga gcagcgacag gatcaggata
      661 tccgctccgg tagtggttgc acgcacgcgt gggctgaaca gacgacgctt cagcaacagc
      721 acgccgccaa tcagacacag cacgccgctg gcaccaccag caaacatcgc cattttctgt
      781 ttcacttcaa tcggcagcca ggcttcatac atccagtgcg gcgtcagcat accgaagaag
      841 tgaccaacaa aaatccccag aataccgata tggaacaggt tcgacgccag gttcatccct
      901 ttgcgatcca gcatctggct ggacgccgcg cgccaggtgt actgcccgta gtcataacgc
      961 agccagctac caatcaggaa gaccgccccg gctatgtacg ggtagatatc aaagaagaac
     1021 atattcagga attgcattag tgctgtcctc cggtggtgat attcagatat tgcggcgcga
     1081 cggcaccggc aaagcgacgc tgatgagcag tgattgctga atcaccgcag cctttgtcag
     1141 caaagaattt aacctgctct tcttcccaga cagcatccag cgcctgcggc gtatcatcgc
     1201 gcgcttcgtc ggcaattttt tccgccactt tgtcgctgtc gatagcggta ttggccaatt
     1261 tcagcagcag atcaaacatc acggcataac ggctttcacg ctgttgcaga cgcgcgctca
     1321 gcaatgccag aatcggcgcg atatctttca aaccttccac ggcttcgctt tgcggcagct
     1381 gcgacaggta ctccagatac agcggcagat ggtccggtaa ttcgcggctg tttaactgca
     1441 agccgtgctg ctcgtactgc gccagcaggt ccaccatcgc ctggccgcgg tcacgggatt
     1501 caccgtgaac atgttcaaat agcaacagtg acgtggcgcg gccacggtcg aacagttcgc
     1561 tgtactgggc ctgggcatcg agcggatcca tcgccgttaa atcgcgcagg aaaatgccca
     1621 gcgcatgggc atcttctttc gacagatttt tcgacgcagc aatcgcctca aacatctctt
     1681 gttgatgctg ccataaggca gcatccggat attcaaggag acgcgataca atcacgagtt
     1741 cgatcatgga tgcggctccg ttttgctggt cacatcgatg gcatcgatac gacggctgtt
     1801 gaacagattg aatttggtat ctgaaccgtg gcagccatca ccaaaggtaa agccgcagcc
     1861 atttttctcc gggaaggctt cccgtgccag ttcacgatga ctactcggca ccacaaagcg
     1921 atcttcgtag ttagcaatcg ccagataacg gtacatctcc tgtgcctggg cttcggtcag
     1981 accgacctct tccagcgcac gggtatcaac tttaccgtca acggtttcag cacgtttgta
     2041 atgacgcatc gccaacatac gtttcagtgc gcgcagtacc ggtttggtat caccggcggt
     2101 cagcagattc gccagatact gtaccggaat acgcaagctt tcgacgtctg gcagaatgcc
     2161 gttgctaccc agctcacccg cgtctgctgc agactgaatc ggagacagag gcggcacgta
     2221 ccagaccatc ggcagtgtgc gatattctgg atgcagcggc agcgccagtt tccattccat
     2281 tgccatttta taaaccggcg actgctgtgc ggcttcaata acgctcagcg gaataccgtc
     2341 tttaatcgcc tgctcgatga ctttcggatc gttcggatcg aggaacacct ccagctgacg
     2401 ctggtaaaga tctttctcgt tctcggtgct ggctgcacgt tcaatcgcgt cggcatcgta
     2461 cagcagcacg ccaagataac ggatacgacc gacacaggtt tctgagcaca cggtcggctg
     2521 acccgcttca atacgcggat agcagaagat gcacttctca gacttaccgc tcttccagtt
     2581 gaagtagatt tttttgtacg ggcatccggt gatgcacata cgccagccac ggcatttatc
     2641 ctggtcgatc aggacgatgc catcttcttc acgcttgtaa atcgcaccgc tcgggcaggt
     2701 cgccacacat gccgggttca ggcagtgttc gcacaggcgc ggcaaataca tcataaaggt
     2761 gttttcgaac tggctataca tcgccttctg gatgttgtcg aagttcttgt ctttcgccag
     2821 tttgtcaaac tcaccaccca ggtcatcttc ccagttcggc cctttttcga ttttcgccat
     2881 ccgttccccg gtaatcagcg aacgcggacg ggcaatcggc tgcgatttgc tgccttccgg
     2941 cgcggtatgc aggttctgat agtcaaaatc gaacggctcg taataatcgt cgatccccgg
     3001 cagatgcggg ttagcgaaga ttttacccag caacatggca cggttaccca tgcgcggctg
     3061 cagtttgccg ttgattttac ggatccagcc gcctttgtat ttttcctggt tttcccagtc
     3121 agtcgggaag ccctgacccg gcttggtttc cacgttgttg aaccacgcgt attccacgcc
     3181 ttcacggctg gtccagacgt ttttacaggt aactgaacag gtgtggcagc cgatgcactt
     3241 atcgagattc agcaccatgc cgacttgtga acgaattttc attttacgct ctcctgtacc
     3301 tggtcattgc cttcgccatc taaccagtca atgttcttca tcttacgcac tacaacaaac
     3361 tcatcgcggt tagaacctac ggtgccgtag tagttaaagc cgtatgccag atgggcatag
     3421 ccgccgatca tatgcgtcgg tttcggcgtg atacgggtga ccgagttatg gataccacca
     3481 cgctgttggg taatttccga accaggcagg ttaacgatac gttcctgcgc gtggtacatc
     3541 atggtcatcc ctgccggaac acgctggctg acaaccgcac gggcagtcag agcaccgttg
     3601 ctgttgaaga cttcaatcca gtcgttatcg gcgataccca gatctttggc atcggcttca
     3661 ctcaaccaga ccaccggacc accgcgacct aaagtcagca tcagcaggtt gtcgctgtag
     3721 gtggagtgga taccccactt ctggtgcggc gtcaggaagt tgagcgcttt ttccgggttg
     3781 ccattggatt tctggcccat cacttctttc accgaacggg tgtcgatcgg cggacgataa
     3841 accagcaggc tttcaccgaa atcacgcatc cattggtgat cctgatacag ctgctgacgg
     3901 ccagagagcg tacgccacgg gatcagctcg tgaacgttgg tgtaaccggc gttgtaagaa
     3961 acgtgttcat cttcaaggcc agaccaggtc gggctggaga tgattttgcg cggctgtgcc
     4021 tgaatatcgc ggaagcgaat cttctcgtct tctttattca gcgccagatg cgtatggtca
     4081 cgaccggtaa actcgctcag ggcagcccag gctttcacgg ctacctgacc gttggtttcc
     4141 ggtgccagcg tcaggatcat ctctgccgca tcaattgcgg tgttcagcat cggctggcct
     4201 ttcgccggac cttccgcttt ggtgtagttg agcttacgca gcagatccat ctcgctctgg
     4261 gtgttccagg caatcccttt accgccatta ccgattttct ccatcagcgg gccgatagag
     4321 gtaaagcgtt cgtaagtcgc cggataatcg cgctctacga ccataatgtg tggcgcggtt
     4381 ttaccaggga tcaggtcgca ctcgcctttt ttccagtctt tcacatccag cggctgcgcc
     4441 agttcagcgg cagagtcgtg ctggataggc agcgtgacga tgtcggtttc tttacccaga
     4501 tgaccgacgc acacttcgga gaatttcttc gcgatggctt tgtaaatttc ccagtcgctt
     4561 ttcgcttccc aggccggatc gaccgccgca gacagcgggt gaataaacgg atgcatatcc
     4621 gaagtattca tgtcgtcttt ttcgtaccag gtcgccgtcg gcaaaatgat gtcggaatag
     4681 agacaggtgc tcgacagacg gaagtccagc gtaaccacca gatccagctt gccttccaga
     4741 ccattgtcct gccagtccac ttcttccggc ttcacgccgc cctgttgccc cagatcttta
     4801 ccctggatac cgtgctccgt ccccagcagg tacttgagca taaactcatg acctttaccg
     4861 gaagaaccga gcaggttaga acgccagatg aacaggttgc gcgggtggtt tttaccgttt
     4921 tctggttgtt ctgccgcaaa acggatggaa ccctctttca gggatttcac cgtatagtcc
     4981 accggattca tcccggcttt ttccgcttcg ccagcgatag tcagcgggtt agtgcctaac
     5041 tgcggtgcag acggcagcca gcccatgcgt tccgcacgga cgttaaagtc gatcaagtgt
     5101 ccggtatagc gggatttgtc cgccatcggt gacagcaact cttccgccgt gacggtttca
     5161 taacgccact ggctggagtg gttatagaaa taagaagtgc tgttcatgtg acgcgccgga
     5221 cgctgccagt caagggcaaa cgccagcggc tgccagccgg tttgcggacg cagtttttcc
     5281 tgacctacat agtgcgccca gccgcccccg ctctgaccga cacagccgca gaaaatcagc
     5341 atgttgatca gaccacgata gttcatatcg aggtgatacc agtggttcag ccccgcaccg
     5401 acgataatca tcgaacgacc gtgcgtttta tcagcgttat cggcaaattc acgggcgatg
     5461 cgaataatct ggctgcgaga aacgccggta atctgctcgg cccaggccgg ggtataagct
     5521 ttcacatcgt catagctggt tgcacagtta acgtcgttca ggccacgttc cagaccgtag
     5581 tttgccagcg tcagatcata aacggtggtc accagggcgg tgctgccatc agccagttgc
     5641 aggcgtttca ccggcagttt gtgcagcagc acgttttcca gttccacttt gttgaagtgt
     5701 tccgtgccgt cgccaccaaa gtacgggaag cctacctcag cgatctcatc ctggctaccc
     5761 agcaggctca gttgcagctc ggtttcttcg ccagttttgc cgtcgcgctg ctcaagattc
     5821 catttgccct tctcgcccca gcggaagcca atagaaccgt tcggcgcaac catttcgcca
     5881 ttggtattaa aggcgacagt tttccattcc ggattgtttt cctggcccag cgcatcaacc
     5941 agatcagcag cgcgcagcat acgacctgca gcgtagtaac cgtcgcgttc ttccagcatc
     6001 accagcatcg gcatgtcggt gtagcgacgc acatagtcgg tgaaatactg gcttgggttg
     6061 tcgaggtgga attcacgcag cattacgtgg cccatcgcca gcgccattgc cgcatcggtg
     6121 ccctgtttcg gtgccagcca cagatcgcac agtttggcga tttcagcgta gtctggtgtg
     6181 acggcaacag ttttggtccc tttgtaacgc acttcagtaa agaagtgagc atccggggta
     6241 cgcgtctgcg gtacgtttga cccccaggcg atgatgtagc tggagttgta ccagtcagca
     6301 gattccggta cgtcagtttg ctcgccccag gtttgcggag acgcaggagg caagtcgcag
     6361 taccagtcgt agaagcttaa gcaagtaccg ccaatcagcg agagatagcg tgcacccgat
     6421 gcgtaagaaa ccatcgacat tgccggaatt ggcgagaaac cagcaacacg gtccgggccg
     6481 tagtttttga tggtgtaaac gttagatgcg gcgatcagtt cgttcacctc ctgccaggaa
     6541 gaacgaacaa atccaccgcg tccacgcgcc tgcttaaagc ttttcgcttt atcggcgtct
     6601 tcaatgatag aagcccatgc ctcaaccgga tcgctatgca gcgccttcgc ttcacgccac
     6661 attttcatca ggcgtttgcg catcatcggg tatttcaggc ggttggcact gtaaagatac
     6721 caggagtagc tggcaccgcg cgggcagccg cgaggttcat ggtttggcag atccggacgg
     6781 gtacgcggat agtcagtctg ctgggtttcc caggtgacca gaccgttttt gacgtagatt
     6841 ttccagctgc aggagccggt gcagtttacc ccgtgggtag agcggacgat tttgtcatgc
     6901 tgccaacgct ggcgatatcc atcctcccag tcacggttgg tattgagaag ctggccatgc
     6961 ccatcggcaa aggtttcacc cttctgtttg aagtagcgaa accggtccag gaatttactc
     7021 atcggttttc tcctgtggga gcctgtcggc cctctgataa atcgacattg ctaatgttta
     7081 agggcaaggt aacgctctga aacagcgtgg gaattgataa cgatcaagtg tgatggggat
     7141 gaaaaataaa gtaaattcct aagatacccc tttaggataa ttaacagtta aagcataaca
     7201 tgctgtaaat taagacgttt tatacctgta gttatttaaa gcatgaagat ttttatgcag
     7261 atgggtatta aggagtattc cccatgggta acatattaat ggctcttaat gaaatccggc
     7321 gaataagcca tataacatta gtatgttgta actaaataac gcataaaaaa agcgcgacat
     7381 catgccgcgc aaaggataat caaatatttt actctatata attcggttta caggaaggcg
     7441 gcaagtgaat gaatcccagg agcttactaa agtaagtgac tggggtgaat gaacgcagcc
     7501 gcagcacatg taacccgaag tatgacgagt aacggtttat tttttagaat gccgaccata
     7561 taccgcccag gtaatcacta cgcaggcgat atagaaaatc aaaaatacct tcattgcgcc
     7621 gactggcgaa cccgttaatg ccagcgagct accaaacgct ttcgggataa agaagccacc
     7681 aatagcgcca atcgcagaga tgaaacccag cgccgctgcc gtgtcggttg ccgcttcacg
     7741 catcgcacgt tcgtcagaac ccccttctgc tttcacgcga tccattgtca gtttacggaa
     7801 gatcactgaa atcatctgga aagtggaacc actacccagc ccagctgtca ggaacagcgc
     7861 caggaagacc gcgaagaacg ccatgaagct tccgccctgc ccgtcagtcg gtaaggtcag
     7921 gaacagcagg ccgctgaaaa tcgccatcag aataaagttc accagcgtga cacgagttcc
     7981 gcccagacgg tcagataatg caccacctgc agaacgcgcc agcgcaccaa taaacggccc
     8041 gaagaaagcg tattgcagaa tctgaacatc cgggaactgc gtttttgaca gcattgcaaa
     8101 gcccgcggag aagccgatga aggagccgaa ggttgccaga tacagcaggc tcataatcca
     8161 cagatgaccc cgtttgagta ccggcaactg ctccttgatg gaggctttcg aggtagcaag
     8221 atcgttcatg ccaaaccacg ccgcaatagt gaagatggca aggaacggca cccatatcca
     8281 ggacgcattc gccagataca gctcagtccc atccggctgt ttgacgccct ggctaccaaa
     8341 tactgcgaaa atcgacagtg ataccaccag cggagcaacc aactgcatga cgctgacgcc
     8401 catgttgccc agaccaccat tcagacccag cgcgccaccc tgcttctgtt tcggaaagaa
     8461 gaagctgatg tttgccatac tggaggcgaa gttcgcgcca gcaaagccgc atagcagaga
     8521 gatgatgatg aagacgctat aaggcgtgga ggtatcctgc acggcaaaac ccagccagac
     8581 gcaaggaata atcagaatac cggtgctgaa cgccgtccag cgacgaccac cgaagatagg
     8641 aaccataaag gagtatggaa cacgtaataa cgcgccagaa accgaaggca gcgcagtcaa
     8701 cataaatagc tgatcggtcg taaaattaaa gccgactttc ggtaggttca ccgcaacagc
     8761 gctgaacaac atccatacgc aaaacgccag cagcagacag ggaacggaaa tccacaggtt
     8821 gcggctggca atacgttgac cgcgttgttg ccagaacgca ggatcttccg gtcgccaatc
     8881 tgtaatgaca gctccagtag ccctttcggg ggcggatgag tgactcatag acacctctga
     8941 tactcgtttc gatgtctgcc accttagtgt ctgtagctaa aggcaatttg atgtaaatca
     9001 aacgataaga taactttatc tatcattgat atttatcatt acccatagtg agtacagtga
     9061 tttcataaaa attatgagat ttttcacggt gctgtagaat ccctaccctt aacgatgtaa
     9121 agcgactaac cacacggcaa ataaggagta actctttcca ggtatgggta tacttcagcc
     9181 aatagccgag aatactgcca ttccagaatg tatcgtcaca ttcattaagg ttattgctca
     9241 tttaaagcct gaaggaagag gtttacatgc ttaaacgttg tctctctccg ctcaccctgg
     9301 ttaatcaggt tgcgcttatt gtgttgcttt ctactgctat tggactggca gggatggcgg
     9361 tttctggctg gctggtgcaa ggcgttcagg gcagcgccca tgcgatcaac aaagcgggat
     9421 cgctgcgcat gcaaagttac cgtctgttgg cagcagtgcc attaagcgag aaagacaagc
     9481 ccttaattaa agagatggaa caaacggcat ttagcgccga gttgactcga gcagcagaac
     9541 gagacggaca actggcgcaa ttacagggtt tacaagatta ctggcgtaat gaactgatcc
     9601 ctgcgttgat gcgtgcacaa aaccgagaaa cggtgtcagc ggatgtcagc cagtttgttg
     9661 ccgggcttga tcaactggta tctggttttg accgcaccac ggaaatgcgc atcgagacag
     9721 tggtactggt ccatcgggta atggcggtat ttatggcact tttactggtg ttcactatta
     9781 tctggttgcg ggcgcgactg ctacaaccgt ggcggcaact gctggcaatg gcgagtgccg
     9841 tcagtcatcg cgattttacc caacgcgcaa acatcagcgg gcgcaacgaa atggcgatgc
     9901 ttggaactgc gttgaacaat atgtctgcag aactggccga aagttatgcc gtacttgagc
     9961 agcgggttca ggagaaaacc gccgggctgg agcataaaaa tcagatcctc tcttttttat
    10021 ggcaggctaa ccgccgtttg cattcccaag ccccgctgtg tgaacgcctg tcacctgtac
    10081 tcaacggctt acagaattta accctgctac gtgatatcga actgcgggtt tatgatactg
    10141 atgatgaaga gaatcatcag gagtttacct gccaaccaga tatgacttgt gatgataaag
    10201 gctgccagct ctgcccgcgc ggcgtattac ccgttggtga tcgcggcaca accctgaagt
    10261 ggcggctggc tgactctcat acgcagtacg gtattttgct ggcgaccctg ccgcaggggc
    10321 gtcatcttag ccatgatcaa caacaactgg tggataccct ggttgaacaa ctcaccgcca
    10381 cgctggcgct ggatcgccat caggaacgtc agcaacagtt gatcgtgatg gaagagcgtg
    10441 ccaccattgc gcgcgaactg catgattcta ttgcccaatc tctctcttgc atgaagatgc
    10501 aggtgagttg tttacagatg cagggcgatg cgctgccaga aagcagccgc gaactgttaa
    10561 gtcagatccg taacgaactg aatgcatcct gggcgcagtt gcgtgaattg ctcaccacat
    10621 tccgcttgca gctcaccgag cctggattac gtccggcgct ggaggcgagt tgcgaagagt
    10681 acagcgccaa atttggcttc ccggtgaagc tggattatca attgccgcct cgcctggtgc
    10741 cttcgcatca ggcaatccac ttgttgcaaa ttgcccgtga ggcattaagt aacgccctca
    10801 aacactcgca agcgagtgaa gtcgtggtga cggtggcgca aaacgataat caggtcaaac
    10861 tgaccgtcca ggataacggc tgcggcgtgc ctgaaaatgc catccgcagc aatcactacg
    10921 gcatgataat aatgcgcgac cgtgcgcaaa gtttacgagg cgattgccgc gtccgccgtc
    10981 gtgaatcagg tggcaccgaa gtggtggtca cctttattcc cgaaaaaact ttcacagacg
    11041 tccaaggaga tacccatgag taatcaggaa ccggctacta tcctgctgat tgacgatcac
    11101 ccgatgctgc gaactggcgt aaaacagctt atcagtatgg caccagatat caccgtggtt
    11161 ggcgaagcga gtaatggcga acagggtatt gaactggcgg agtctcttga tcccgatctg
    11221 atcctgttag atctcaatat gcccggcatg aacggtctgg aaacgctgga taaactgcgc
    11281 gaaaagtccc tctcagggcg cattgtggta ttcagcgtct ctaaccatga agaagatgtg
    11341 gtcaccgcac tgaaacgcgg cgcggatggt tatctgttaa aagatatgga accggaagat
    11401 ctgctgaaag cattgcatca ggcagctgct ggcgaaatgg tattaagcga agcattaacg
    11461 cctgttctgg ccgccagctt gcgcgctaac cgtgccacta ctgagcgcga tgttaaccag
    11521 ttaaccccac gcgagcgcga tattctcaag ctgattgccc agggtttgcc gaacaagatg
    11581 attgcccgcc gcctggatat caccgaaagc acagtaaaag tgcacgtcaa gcacatgctg
    11641 aagaaaatga agctcaagtc tcgcgtggaa gcagcggtat gggtgcatca ggagcgcatt
    11701 ttctgattac ggttcccagc gcagttcgtc gtttgacaat gcatcgaacg gttcgacaag
    11761 cgttagcgtg atctcattgg aggagacacg ctgcccctgg ttatcttcca ccaccaccga
    11821 caatcgccag tgattgcttg ccccttcccc gttctgccag tcaggcatga tcagcgtcca
    11881 gccctccgcg ctgttggctt gtgcgcccgg cgtcaaactt aatatctgcg tatcgccctg
    11941 ccaaatcagt tgccgaatac cgtaacgact gcggatttgt aatttcagcg gcactgtttc
    12001 gccaggtttt agatcccacg gcggtgtcgc cagaaacacc gttaacgttt ttcgctgtcg
    12061 gtactcaaga gtcggtagat tatttcgctg cggattgtca tagcgactac cacgtaacga
    12121 ctgactttcg gcaacctcac ccgcagaaag ttgttttttg agcggtacac caaagcggta
    12181 attaagattc agcccgaggt tattttgatt ctcgccactt tcaccctgtt tatgctgggc
    12241 cgtcacagtg actaatggca caggggtgta atttaatccc agactcaacg cgacgggatt
    12301 gtgataaccc gtaccagagt taaacaaatc aacacgatca ccaaaatact gttctacgct
    12361 gacactggta ttgaggtgtt gatagaacgg catgcgcatc cgggctgtca ggtcataccc
    12421 gcgcgccatc cgttgttcct gcgtggctgt ctgttcatgc catgcagcaa acggctgata
    12481 aaagtttgcc gatagtcgca aatattcgcc ccacgcttcg gcaccaaagc ccgctcgctg
    12541 aagattttcg tccagcaagt tgtcataaaa agtgttataa cccaccagcc agttgccgcg
    12601 cgcccagcgt tgcccaacgc ccacattgct caccaaccca tcatcctgct gagtaagacc
    12661 aagctggctc caggtgagat aacgatcatt atcttgtaac ggcacaaacc agcttccacg
    12721 actgccggtg aaatgtcctt cgttatccac tttgacgtca acactggcat ttccccacgg
    12781 tgatagccag gactctacgt gctgattaac ctgttgacta agcgcgtcgc ggacttttcc
    12841 caatgcgaaa gcttttgcct gctcgccagt atccagcccg ttatcattca tactggtttc
    12901 gccgaaatct ttcacaattt cagcaaagtg tttttcccca tcatgatttt cgggagccat
    12961 gcctaaatcc ggcagaccat cattgttatt atcaaaggga tttgccgctt tttgctcgaa
    13021 ggtagattgt gcgttagctg taccgcctgc tacaagcaag agtaaataaa acgggataat
    13081 gcgaggaacg aaacggctca aaacgtcggt aacttccggt aataatgaaa aaccatagcc
    13141 ttaaagaata acgattactg gcaacttttg tcgtattttc tcgcgctttc aggaaaatcc
    13201 ttaaaaatgt gaccgattgg tcagacgctg cagaagcagt aagatagaag atgaatgtct
    13261 aattccctga atacaggagt aaataatgca aaaaatcgtg atcgttgcca atggcgcacc
    13321 ttacgggagc gaatccttgt ttaacagctt gcggctagcc attgcgttac gagagcagga
    13381 gagcaatctg gatctgcgtc tgttcctgat gtctgatgcg gtcacagccg ggttgcgcgg
    13441 gcaaaaacca ggggaaggct acaacattca gcaaatgctg gagatcctta ccgctcagaa
    13501 tgtaccggtg aaattgtgca aaacctgtac cgacgggcgc gggattagta cacttcctct
    13561 gattgatggg gtggaaatcg gtactcttgt ggaactggcg caatggacgc tgtcagccga
    13621 taaagtgctc acattttaat gtttgagggg gcgtctgacg ccccactatg agggaagttt
    13681 actcaggcga atcccggccg taacacacca tccggataat tctccgccag cagttttttt
    13741 accgatacca gcaaatcatt cagcccatcg tcctgcattc ccagtttgat aagctcctgt
    13801 tccagtgaaa acaggtattg tgcgttggtt cccagcggac cgctcgccgc cgcaatcaac
    13861 ggcgcgatga cctgagcgcg agtgtcagat tcatattctg gatgtcgcgg gtccataata
    13921 aacacaatgg cgtttactgt gcatccatca tcaagatcaa gctgacacca ggttggcaga
    13981 taacagccgg taatcatctc gcgcttccac aacagggtta gttcctgctc cagcgtctct
    14041 tctggcagtc gataggcgac gcctgtagtg cgtccgccct ctttcagtgc aagcatccgt
    14101 cccggctggt gcgcagtccc gcgcccggcg gtcaggcgca ggcaaaacgc gcgatgccat
    14161 ccaaccagtg taccggtgca cgactcggta aactccagtg ccggattcca catcaacgat
    14221 ccataaccga agatccacac cggtccctca tcaggtcgac aagccagcgt cgccgccagc
    14281 gaagccgccc gttgctctgc tgaccataag agtgattctt caatggcacc aaatgccgtt
    14341 ttacaatcgg cattcatcaa gaaatcacgc gttatcacct tacacctccg ccactgcata
    14401 cttccctgcg acaacttttt ggtgtcttcc ggacattttt actgctcatt ttgaaaccac
    14461 taacgggaca atatgcaatc cacggaccac ttgcaaggca acaccgcacg aatagcttta
    14521 actaagctac cggttttacg attttttatg ccatttatca tcatcccctt tggcatattc
    14581 atgcttcaca gcagcccagg ccactttatg cgcggtttct tcgcgactgg cgtcatcacg
    14641 ccgatcgtct ttatctttat attgatccca tgcgctgttg aacgcttctt tatagatatc
    14701 ctgggcatga gacggtagaa cgtgctttac gctttccggc agatcgcttt tcgttttata
    14761 cggcatcgtt gactcctttt acaaagttga acattaagtg tggtcaacaa tatctactct
    14821 tg
//
LOCUS       DAZGOB010000129        14767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGOB010000129 DAZGOB010000000
VERSION     DAZGOB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.129"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..526
                     /gene="yehD"
                     /locus_tag="ACHLIF_004043"
                     /pseudo
     CDS             1..526
                     /gene="yehD"
                     /locus_tag="ACHLIF_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
     gene            607..1281
                     /gene="yehC"
                     /locus_tag="ACHLIF_004044"
     CDS             607..1281
                     /gene="yehC"
                     /locus_tag="ACHLIF_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9607953.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            1297..3777
                     /gene="yehB"
                     /locus_tag="ACHLIF_004045"
     CDS             1297..3777
                     /gene="yehB"
                     /locus_tag="ACHLIF_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO9607954.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAASYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3791..4825
                     /gene="yehA"
                     /locus_tag="ACHLIF_004046"
     CDS             3791..4825
                     /gene="yehA"
                     /locus_tag="ACHLIF_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO9607955.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKRYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(4907..5245)
                     /gene="rcnB"
                     /locus_tag="ACHLIF_004047"
     CDS             complement(4907..5245)
                     /gene="rcnB"
                     /locus_tag="ACHLIF_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO9607956.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5464..6188)
                     /locus_tag="ACHLIF_004048"
                     /pseudo
     CDS             complement(5464..6188)
                     /locus_tag="ACHLIF_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6309..6581
                     /gene="rcnR"
                     /locus_tag="ACHLIF_004049"
     CDS             6309..6581
                     /gene="rcnR"
                     /locus_tag="ACHLIF_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO9607957.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6673..6769
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6804..7592
                     /gene="thiM"
                     /locus_tag="ACHLIF_004050"
     CDS             6804..7592
                     /gene="thiM"
                     /locus_tag="ACHLIF_004050"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO9607958.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7589..8389
                     /gene="thiD"
                     /locus_tag="ACHLIF_004051"
     CDS             7589..8389
                     /gene="thiD"
                     /locus_tag="ACHLIF_004051"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO9607959.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8454..9272
                     /gene="yegX"
                     /locus_tag="ACHLIF_004052"
     CDS             8454..9272
                     /gene="yegX"
                     /locus_tag="ACHLIF_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO9607960.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            9324..10070
                     /gene="yegW"
                     /locus_tag="ACHLIF_004053"
     CDS             9324..10070
                     /gene="yegW"
                     /locus_tag="ACHLIF_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9607961.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10044..11009)
                     /gene="yegV"
                     /locus_tag="ACHLIF_004054"
     CDS             complement(10044..11009)
                     /gene="yegV"
                     /locus_tag="ACHLIF_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO9607962.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11006..12010)
                     /gene="yegU"
                     /locus_tag="ACHLIF_004055"
     CDS             complement(11006..12010)
                     /gene="yegU"
                     /locus_tag="ACHLIF_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO9607963.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(12007..13284)
                     /gene="yegT"
                     /locus_tag="ACHLIF_004056"
     CDS             complement(12007..13284)
                     /gene="yegT"
                     /locus_tag="ACHLIF_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO9607964.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13541..14593
                     /gene="fbaB"
                     /locus_tag="ACHLIF_004057"
     CDS             13541..14593
                     /gene="fbaB"
                     /locus_tag="ACHLIF_004057"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO9607965.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         3964 a         3472 c         3717 g         3614 t
ORIGIN      
        1 ttgctgccgc tgtcttttct tcttttttat gagcgctgga gtatttgctg cagacgttga
       61 taccggaaca ttaactatta aggggaatat tgcagaatct ccgtgtaaat tcgaagcggg
      121 tggtgattca gtaagtatta atatgccgac tgtaccaacc actgtctttg aaggtaaagc
      181 taaatattct acctatgatg atgcagtcgg tgtaaccagc agcatgttaa aaattagctg
      241 cccgaaagaa gttgctggtg taaaactctc gttgattacc aacgataaaa taaccggtaa
      301 cgataaggag atagccagta gcaacgatac cgtgggttac tatctctatt taggtgataa
      361 cagcgatgtc ctggatgttt ctgcaccttt taacattgag agttataaaa cagcggaagg
      421 tcaatatgct attccgttta aagcaaaata cctgaaactg acagataact cagtgcaatc
      481 aggtgatgtg ttatcttctc tggttatgcg tgtggcgcag gattaataaa taaaattgta
      541 agtttctcca ggtaataaac atggccgcta tccccatggc ggctttttaa tttaagaggc
      601 attaagatga aaggattatt atctttactc attttttcta tggtccttcc tgcacatgcc
      661 ggaattgtta tctacgggac gcgcattatt tacccggcag aaaataaaga agttatggtg
      721 cagttgatga accagggaaa ccgttcttcg ctgctgcagg cgtggattga tgatggcgat
      781 acgtcgttac caccagaaaa aattcaggtt cctttcatgt taacgccacc agtggcaaaa
      841 ataggggcaa attccgggca gcaagtaaaa atcaaaatta tgccgaataa actgcccact
      901 aataaagaaa gcatttttta tctgaatgtt ctggatattc caccaaatag tccagagcaa
      961 gaaggcaaaa atgcactgaa gtttgcgatg caaaacagaa ttaagttgtt ttaccggcca
     1021 gcgggtattg ctccggtaaa taaagcgacc tttaaaaagt tactggtaaa tcgcagtggc
     1081 aatggattgg tgataaaaaa tgactcagct aattgggtga cgatttcgga tgtcaaagct
     1141 aataatgtta aagtcaatta tgaaactatt atgattgccc ccttagaaag tcagagtgtt
     1201 aatgtcaaaa gtaataatgc aaataactgg tatctgacca ttatcgatga ccacggcaac
     1261 tatattagtg acaaaattta attgcaggag ctgcctatgt tgagaatgac cccacttgca
     1321 tcagcaatcg tagcgttatt gctcggcatt gaagcttatg cagctgaaga aacctttgat
     1381 acccatttta tgataggtgg aatgaaagac cagcaggttg caaatattcg tcttgatgat
     1441 aatcaaccct taccggggca gtatgacatc gatatttatg tcaataagca atggcgcggg
     1501 aaatatgaga ttattgttaa agacaacccg caagaaacat gtttatcaat agaagttatc
     1561 aagcggttag gcattaatag cgataacttc gccagcggta agcaatgttt aacatttgag
     1621 caacttgttc agggtgggag ctatacctgg gatatcgggg tttttcgtct cgatttcagt
     1681 gtcccgcagg cctgggtgga agaactggaa agtggctatg ttccaccgga aaactgggag
     1741 cggggtatta atgcgtttta tacctcttat tatgtgagtc agtattacag tgactataaa
     1801 gcgtcgggta atagtaagag tacatatgta cgttttaaca gcgggttaaa tttactgggg
     1861 tggcaactgc attctgatgc cagtttcagt aaaacaaata acaatccagg ggtgtggaaa
     1921 agcaataccc tgtatctgga acgtggattt gcccaacttc tcggcacgct tcgcgtgggt
     1981 gatatgtaca catcaagcga tatttttgat tctgttcgct tcagcggtgt gcggttgttt
     2041 cgtgatatgc agatgttgcc taactcgaaa caaaatttta cgccacgggt gcaggggatt
     2101 gctcagagta atgcgctggt aactattgaa cagaacggtt ttgtggttta tcagaaagag
     2161 gttcctcctg gcccgttcgc gattacagat ttgcagttgg ccggtggtgg agcagatctt
     2221 gatgtcagcg tgaaagaggc ggacggctcg gtaaccacct atctggtgcc ttatgcagca
     2281 gtgccaaata tgctgcaacc cggcgtatcg aaatatgatt ttgcggcggg tcgtagccat
     2341 attgaagggg cgagcaaaca aagtgatttt gtccaggcgg gttatcagta tggttttaat
     2401 aatttattga cgctgtatgg tggctcgatg gtcgcgaata attattacgc gtttactttg
     2461 gggactggct ggaatacacg cattggtgcc atttccgtcg atgccacgaa gtcgcatagt
     2521 aaacaagaca acggcgatgt gtttgacggg caaagttatc aaattgccta caacaaattt
     2581 gtgagccaaa cgtcgacgcg ttttggtctg gcggcctggc gttattcatc gcgtgattac
     2641 cggacattta atgatcacgt ttgggcaaac aataaagata attatcgccg tgatgaaaac
     2701 gatgtctatg acattgccga ttattaccag aacgattttg gccgtaaaaa tagcttctct
     2761 gccaatatga gccagtcatt gccagaaggc tgggggtctg tgtccttaag tacgttatgg
     2821 cgagattact gggggcgtag cggcagcagt aaggattatc agttgagtta ttccaataac
     2881 tggcggcgga taagctatat cctcgcggca agccaggcgt atgacgagaa tcatcatgaa
     2941 gagaaacgtt ttaatatttt tatatcgatt ccctttgatt ggggtgatga cgttacgacg
     3001 cctcgtcggc aaatatatat gtctaactca acgacgtttg atgatcaggg gtttgcctca
     3061 aataatacgg gattatcagg aaccgttgga agccgggatc agtttaacta tggggtcaac
     3121 ctgagttatc agaatcaggg aaatgaaacg acagctgggg cgaatttgac ctggaacgcg
     3181 ccggttgcga cagtgaatgg cagttatagt cagtcgagta cttatcgaca ggctggagcc
     3241 agtgtttcag ggggcattgt cgcctggtcg gggggcgtta atctggcgaa ccgtctttcc
     3301 gaaacgtttg ctgtgatgaa tgcgccagga attaaagatg cttatgtcaa tgggcaaaaa
     3361 tatcgcacaa caaaccgtaa tggagtggtg gtatacgacg gaatgacacc ttatcgggaa
     3421 aatcacctga tgctggatgt gtcgcaaagc gatagcgaag cagaattacg tggcaaccgg
     3481 aaaattgccg cctcttatcg cggcgcggtt gtactggtta attttgatac cgatcagcgc
     3541 aagccctggt ttataaaagc gttaagagcg gatgggcaac cattaatgtt tggttatgaa
     3601 gtcaatgata tccatggtca taatattggc gttgtcggtc agggaagcca gttatttatt
     3661 cgcaccaatg aagtaccgcc atcggttaat gtagcaattg ataagcaaca aggactttca
     3721 tgcacaatca ccttcggtaa agagattgat gaaagtagaa actatatttg ccagtaatag
     3781 tcaggtggtt atgaatattc gtataatgtt atttatagta atgatgatgg ttatgcctgt
     3841 gagctatgcg gcatgttata gtgagttatc tgttcagcac aatttggttg ttcaggggga
     3901 ttttgcactt actcaaacac aaatggcgac atatgagcat aattttaatg attcgtcatg
     3961 cgtgagtaca aatactatca cccctatgag cccgtcggat attattgttg gactttataa
     4021 cgataccatt aaattaaatt tacattttga atggaccaat aaaaacaaca tcacgttatc
     4081 aaataatcag accagtttca ccagtggtta ttcggtaacg gtgacacctg cggccagtaa
     4141 tgcaaaagtg aatgtttctg cggggggcgg cggttcagtg atgattaatg gagttgcgac
     4201 attatccagt gcttcatcat cgacacgcgg gagtgccgca gtacaatttc tactgtgttt
     4261 attaggtggc aagtcatggg atgcatgtgt aaatagctac agaaatgcat tggcacaaaa
     4321 tgcaggtgtc tattccttta atctgacatt gtcatacaac ccgataacca caacctgcaa
     4381 accggacgat ttattaatta ctttagacag tattcccgtt tcacaattac cagccacagg
     4441 taacaaagca acaataaata gtaaacaagg ggatattatt ctgcgttgta aaaatttatt
     4501 aggtcaacaa aatcaaacat cacggaaaat gcaggtgtat ttatcaagtt ctgacttgtt
     4561 aaccaacagc aacacaatac tgaaaggtgc ggaagataat ggcgtaggat ttattcttga
     4621 aagtaatggt tcgtcagtca cacttttaaa tatcactaac agcagtaaac gatacacaaa
     4681 tttaaaagaa attgcggcaa agtcaaaact tacagataca acggtttcaa ttccgataac
     4741 tgccagttac tacgtctacg atacaaacaa agttaaatct ggcgcactgg aggcaaccgc
     4801 attaatcaac gtgaaatacg actaataaca agcaagacga gcaagtggct aataataaaa
     4861 aataacaagg tgtctgcatt tccccctcat gatgaggggg ctttttttag cgatgataaa
     4921 aaatctcacc gtcgtaggct ttaatgattt taccgtcggt gtcgctgatc aacacgtacg
     4981 cgccacccat ataggtccag tgcgtcccgg catcaggcgc gggcaggtta cgtaactgcc
     5041 actgcttaat gttgtactca tcggtgcggt acatctctgg gacggtatcg cctggtttga
     5101 aatgcttaaa gtcagcaatg aaactgctta actcatactt agaaattccc gaggtattgg
     5161 tcgaacccgc ggtggctggt gcggcccagg cggcactggt aaccagcaaa agcgcaccca
     5221 gcaacatctt atttttaatg gtcataaact ctccactttg gggatctgta atatcagtcg
     5281 catatctttc ctgccataca atataactga caagcgatat tttgttagtg atcccatgat
     5341 ttcgtaaaaa gtgtaaatga aaggatgccc taaaagcatc cttagcgagc agagagtcag
     5401 cggaaatagc tgatttaaga atcatcctgg gctgaagata acatgcaagc ctaaaggatt
     5461 gagttatcgc attatgccca tgaagccatg tacgcccata tacacaccga ctaagccaat
     5521 caacagactg gaaaaatagg gggcgcgttt agcgagagtg ttaaatccgc tccagcgttt
     5581 tgcgacctgc tgaacgctga ttgctgcgcc aacgcctacg gtgacaagcg ttaacgccag
     5641 gccaatgctg aaactgacga ccagtgttgc gcccagtgtc agggctttca actgaatgca
     5701 tatcaacagc acggtaattg ctgccgggca ggggataagg ccaccggtta agccaaataa
     5761 caaaatttgc cagttggtga cctctctacc atcaaagcgt cgtttaatgt cattagcatg
     5821 ggctcgtgca tattctccag ccagttgcgc tcgccgtgcc aggtacgcca gaacatccag
     5881 aacgcggtgc taataatgat cactgcggaa atcaactgga gccacggttc tgctgattga
     5941 gcagtaaagc gcttgctgac caccatcccg ccaaaggcaa tcaaccagac cactgcggta
     6001 tgcgaaatag ttgctgccag tccgagcatc accgcctgtt taatggtgcc tttgatggcg
     6061 atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg aagcgcacca
     6121 agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag agttgtaaat
     6181 tcggtcatga taataattct tagtattaat tcggcaatct gattctactc ccccccagta
     6241 cctgatacta ccccccagta gattaatagt gctatgattt ttcatattct tgttaaccag
     6301 gtgttgccat gtctcataca atccgtgata aacagaaact gaaagcgcgt gccagtaaga
     6361 ttcagggcca ggtcgtggcg ctcaagaaaa tgctcgacga accgcacgaa tgcgctgcag
     6421 ttttacaaca gattgctgct atccgtggcg cggtaaacgg tctgatgcgg gaagtgatta
     6481 aaggtcatct gacggaacac atcgttcacc agggggatga gctaaaacgt gaagaagatc
     6541 tggatgtcgt tctgaaggtg ctggattcat atatcaaata atttattaac gcgattgtaa
     6601 aactgccgtt tttcctcgtt tacaacgcgt gcgctggaca ttaccatcct cctctgcgat
     6661 ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt gaaggctgag aaatacccgt
     6721 atcacctgat ctggataatg ccagcgtagg gaagtcacgg accaccaggt cattgcttct
     6781 tcacgttatg gcaggagcaa actatgcaag tcgacctgct gagttcagcg caatctgcgc
     6841 acgcgttaca cctttttcac caacattccc ctcttgtgca ctgcatgacc aacgatgtgg
     6901 tgcaaacctt tactgccaat accttgctgg cgctcggtgc atcgccagcg atggttatcg
     6961 aaaccgaaga ggccagtcag tttgcggcta ttgccagtgc gttgctgatt aacgttggca
     7021 cgctgacgca gctacgcgca cagtcgatgt gtgccgccgt tgagcaagca aaaagctctc
     7081 aaacaccctg gacgcttgat ccagtagcgg ttggtgcact cgattatcgc cgccgttttt
     7141 gtctggaact tctgtctcat aagccaaccg ccatacgtgg taatgcttcg gaaatcatgg
     7201 cattagctgg cattgctaat ggtggacggg gagtggatac cactgacgcc gcagctaacg
     7261 cgatacccgc tgcacaaaca ctggcacggg aaactggcgc aatcgtcgtg gtcactggcg
     7321 agatggatta tgttaccgat ggccatcgcg ccgttggcat tcacggtggc gatccgttaa
     7381 tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt tgtcgctgcc tgctgtgcgt
     7441 taccaggcga tacgctggaa aatgtcgcat ctgcctgtca ctggatgaaa caagccggag
     7501 agcgcgcagt cgccagaagc gaggggccag gcagttttgt tccacatttc cttgatgcgc
     7561 tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt aacgctctga cgattgccgg
     7621 tactgatccc agtggtggtg cggggattca ggccgatctt aaaaccttct cggcacttgg
     7681 cgcttatggt tgctcagtta ttactgcact ggtggcgcaa aatacccgtg gcgtacagtc
     7741 ggtgtatcgc attgagcctg attttgtcgc cgcccagctc gattcggtgt tcagcgatgt
     7801 gcgaatcgac accactaaaa tcggtatgtt ggcggaaacc gatattgttg aagcggtggc
     7861 agaacggttg caacgttatc agatccaaaa cgtggtactc gacaccgtta tgctggcaaa
     7921 aagcggcgac ccgctgcttt caccttcggc ggttgctacg ctgcgcagtc gattattgcc
     7981 acaggtttca ttaataacgc caaacttgcc cgaagctgcc gccttgctcg acgcgccaca
     8041 cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg ctgttggcga tgggctgtgg
     8101 cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa agcccggact ggctgtttac
     8161 ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg accaaaaaca cccacggcac
     8221 tggttgtacg ctctctgcgg cgttggctgc actacgcccg cgccatacaa actgggctga
     8281 caccgtacag gaggcaaaaa gctggctttc atcagcatta gcccaggccg acacgctgga
     8341 agttggtcac ggtattggtc cggttcacca cttccacgcc tggtggtgag caccgtacta
     8401 tagctgcttt ggttattgtc agcctttggc actgacattc agggatgaaa ccaatgcagc
     8461 taagaattac cagtcgaaaa aaactcacct ctctattatg cgctcttggg ctaatttcca
     8521 tcgttgcaat ttatccgcgt cagacggtga attttttcta ttcgacagca gttcagatta
     8581 cagactacat ccatttctac ggttatcgcc cggttaaatc tttcgctatt cgcattcctg
     8641 ccagttacac cattcacgga atagatgttt ctcgctggca ggagcggatc gactggcagc
     8701 gggtggcaaa aatgcgcgac aacggtatcc gcttacagtt tgcttttatt aaggcgacgg
     8761 aaggcgaaaa gctggtggac ccgtattttt cgcgtaactg gcaactaagc cgcgaaaatg
     8821 gcctgctgcg cggggcgtat cattatttct ccccgtcggt agcagcgccg gttcaggcac
     8881 gattatttct gcaaacggtg gatttctcac aaggcgattt ccctgcggta ctggacgtag
     8941 aagaacgggg aaaattatcg gcaaaagaat tacgcaaacg agtaagtcag tggctaaaaa
     9001 tggtcgaaaa aagtacggga aaaaagccga ttatttactc aggagccgtt ttttatcaca
     9061 ccaatctggc gggctatttc aatgaatatc cgtggtgggt ggctcactat tatcaacgtc
     9121 gtccggacaa tgacggcatg gcctggcgct tctggcagca ttccgaccgt ggacaggtag
     9181 atggcatcaa tggtccggtg gattttaatg tatttcatgg cacggtggag gagttgcagg
     9241 cattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata ctcccaccag
     9301 actccccggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt gcccaactga
     9361 acgagcgtat tttagcggcg gataacacgc cgctttatat taagtttgcc gaaacggtaa
     9421 aaaatgccgt gcgcagcggg gtgctggagc atggcaatat tttgcccggt gagcgtgatt
     9481 taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag gcgctggaag
     9541 aagagggtgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac atcttcgaat
     9601 actcgttgaa agaagcgcga ggtttttctc agcaggtggt attgcgcggg aaaaagcccg
     9661 atacgctatg ggttaacaag cgtgtcgtga aatgtcccga agaagtcgcg cagcagctgg
     9721 cggtcgaagc aggaagtgat gtctttttgc ttaagcgtat tcgctatgtc gatgaagaag
     9781 cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt gatgccatcg
     9841 ggatttcact ttatgactat ttccgcagcc agcacattta cccacaacgt acgcgttccc
     9901 gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta gatagcaaaa
     9961 taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct attgagtaca
    10021 gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag ttcctcgcgc
    10081 gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc attgcccagt
    10141 aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc acctgcatga
    10201 ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat gcagccagaa
    10261 gcgtcgttgc tgaaatacca tgcaccctct ttatcgagac gaacgatcaa cggcgcggca
    10321 aatttctcct gccattgctt gccaagtgtt gttatctctg cggataaagc aaaacgttcg
    10381 gcggcaatct cagcctcttg acgattgagt gatactaaag gtcgacaggc catgatccgc
    10441 gccagtaatg cgtccgggat atcgccgata cgtgggccaa aatcgataaa cggcgtcacg
    10501 tcttgcagct tttccagcca ttccactaac aattcgccgc agggcgaggc cagttgataa
    10561 ccggaaaaat agagcaggct gccaggcgca acggttaatc gcgccagcca ctggcgattc
    10621 cactgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg ctcaaccagc
    10681 gccagacacc agccgttatc gccttcggcg ttatcgatca ggctgattaa cccctctttc
    10741 gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa ggcattaccc
    10801 gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc gccaacgtta
    10861 acgctctgct gcttcagttc gatatcgcac ccacgccagg ggagggcata agcgtcggcg
    10921 atcacatcaa tgaccgccgc gccgacgacc atcaccggct gacgcgaggt taattcaggc
    10981 agcagcgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga tatttcgcca
    11041 gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagttcc gcctttaatg
    11101 caggatcgat agcgttaacg ccatgcaacg cgccgcaaat cgccgtcgcc atagcgccga
    11161 tggtgtctgt gtcgccgcca aggttagcgc acaggacggc gcagcgattc gggtcggttt
    11221 gtgccagttc aaccagcgca atggcgcacg gaacggactc aatagtgctg gtacctgcgc
    11281 caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcattg cgcacaattt
    11341 tcagcgcaat ctccagacgt gctgccagtg aggcgctgaa ggtagtgatg cgtttttgct
    11401 gtgcatgtcg cgcaattgaa ggcagggaat caacaatcgc tgaccagctt tctccgtcaa
    11461 tggcacgaga aatcgcccat gcgatgacta ccgcacctgc aactgccaga tcggatttat
    11521 gggtcgggct cgacgccagc gccacatcat caataaagga atcaacatca cgcgccggaa
    11581 gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggtcacgccg ttgttttcca
    11641 gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag gtcggaccta
    11701 gtacgttttt gttaaaggcg tcgaaacgca gcgcccagtc gagaatatta cgcccaatca
    11761 gatccggatc gatcttgcct tcacgttcca gtaacgcatc cgccagacac agcgccatcg
    11821 aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc cgcgttattc tcctttggtc
    11881 caggcagaaa acggtcaatc cagccaaagt gtgctttaac gcggctgcgt ggccaaagct
    11941 cggagggcat ccccatcgca tcccctaacg cctgcccata aagagcaccg agaatacgtt
    12001 ctgttttcat ttaacttccc cttgtgtcaa cgcaatatcg cgatcatcga ccttgatagc
    12061 cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac agcacggcga taatggcaat
    12121 catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa gtcagcccgt ttaccggttc
    12181 cggataagcg aacatctttt ccatcatcac accgccaaga cgatagccga gcaaactgcc
    12241 gaagccctgg cagcagagcg tgatcagccc ctgcgcggcg gtacgcatat gcacgggggc
    12301 ttttttatcg acatagatgt aagcggtaac gtagtaaaaa tcgtaactta cgccgtgaag
    12361 caaaataccg aggaacagta acgcgtaggt gaaatattca tccgcactac cgtaaataaa
    12421 gaagccatag cggatcgcag cggtgaccag accaagcaat aatacctttt tgataccaaa
    12481 gcgtttagtg aaaaacggca atgccagcat aaagaagatt tcagagaact ggccgagcgt
    12541 catccagccg gtggcgtttt tcatgccaac ttcggtcaga taaccgttgg caaagatgta
    12601 atagaacgcc agtggcatcg caaacaggaa tgaacagaag aaaaagacga ggaagttttt
    12661 atcgcgcagc aggatcagcg catccaggcc gagcatgact ttaatgtcca ttttgccggt
    12721 gctttttggc ggcgtgtcgg gcaggaaaaa cgcaaacaca ccgagcagag cagaacttcc
    12781 ggcggtaatc agcagcggga tgttagtcgg tgagatatcg gcatacccca gcatttgcgg
    12841 caagaaacca catgccagac cagaggcaat ccagccgata gtgcccatca cacgaatgcg
    12901 cgggaaatca cgctccacat ccggcacgtt ggcaaaagcg atactgttag tcagcgcaat
    12961 ggtcggcata taggttagcg agtaggccag cagtaacggg aagaacccgg caaaagtggt
    13021 ctgttgcgca gcgaaataca tcagcagcgc gcctgcgaac atcaataccg ccagcacttt
    13081 ttgcgccgag aaaaagcggt cagtgatgga gccaaccaga attggcgaca ggatcgccgc
    13141 aatggctgtg caggcatacg accagccaat ttctccggca ctaaaaccgc ttttacttaa
    13201 ccacaaccac aatggcacaa accacgcgcc ccagataaac cattcaacaa acatcatgaa
    13261 cgacagcttt gctgttgttt tcatctttta attccttcat gacaggtaat gtaaggtacg
    13321 cgatgacaat accacttaat aatacctttt aaataccttt gcggtgaaaa cttgaccgcg
    13381 ctaacatttt ttctgatgaa tcgagccaac agaaaacgct gaaaaaacat ccaaaagatg
    13441 gaaaaactcg cccgtaacga ccaggattat gattgcccgc aaaaggcggg ccaggacaat
    13501 ccatagccga tatccaatcg gaatttacgg gagcatagta atgacagata ttgcgcagtt
    13561 gcttggcaaa gacgccgaca accttttaca gcaccgttgt atgacaattc cttctgacca
    13621 gctttatctc cccggacatg actacgtaga ccgcgtaatg attgacaata atcgcccgcc
    13681 agcggtgtta cgtaatatgc agacgttgta caacaccggg cgtctggctg gcacaggata
    13741 tctttctatt ctgccggttg accagggcgt tgagcactct gccggagctt catttgctgc
    13801 taacccgctc tactttgacc cgaaaaacat tgttgaactg gcgatcgaag cgggctgtaa
    13861 ctgtgtggcg tcaacttacg gcgtgctggc gtcggtatcg cggcgttatg cgcatcgcat
    13921 tccattcctc gtcaaactta atcacaacga gacgctaagt tacccgaata cctacgatca
    13981 aacgctgtat gccagcgtgg agcaggcgtt caacatgggc gcggttgcgg ttggtgcgac
    14041 tatctatttt ggctcggaag agtcacgtcg ccagattgaa gaaatttctg cggcttttga
    14101 acgtgcgcac gagctgggta tggtgacagt gctgtgggcc tatttgcgta actccgcctt
    14161 taagaaagat ggcgttgatt accatgtttc cgccgacctg accggtcagg caaaccatct
    14221 ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg gcggaaaata acggcggcta
    14281 taaagcaatt aattacggtt acaccgacga tcgtgtttac agcaaattga ccagcgaaaa
    14341 cccgattgat ctggtgcgtt atcagttagc taactgctat atgggtcggg ctgggttgat
    14401 aaactccggc ggtgctgcgg gcggtgaaac tgacctcagc gatgcagtgc gtactgcggt
    14461 tatcaacaaa cgcgctggcg gaatggggct gattcttgga cgtaaggcgt tcaagaaatc
    14521 gatggctgac ggcgtgaaac tgattaatgc cgtgcaggac gtttatctcg atagcaaaat
    14581 tactattgcc tgatacgcct catacgaaca gcaccacccc gtaggacgga taaggcgttt
    14641 acactgcatc cggcaaccta tgcctgatgc gacgcggaag cgtcttatca tgcctacata
    14701 gcactgccac gtatgtttac accgcatccg gcataaaaac acgcgcactt tgctacggct
    14761 tccctat
//
LOCUS       DAZGOB010000130        14501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGOB010000130 DAZGOB010000000
VERSION     DAZGOB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.130"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            107..673
                     /locus_tag="ACHLIF_004058"
     CDS             107..673
                     /locus_tag="ACHLIF_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9607966.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            724..3183
                     /gene="ybgQ"
                     /locus_tag="ACHLIF_004059"
     CDS             724..3183
                     /gene="ybgQ"
                     /locus_tag="ACHLIF_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9607967.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNDVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3198..3929
                     /gene="ybgP"
                     /locus_tag="ACHLIF_004060"
     CDS             3198..3929
                     /gene="ybgP"
                     /locus_tag="ACHLIF_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9607968.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNIGNYSGSQFYLGYMD
                     DYGALRMTTLNYSGQCHLQAVEAKK"
     gene            3926..4987
                     /gene="ybgO"
                     /locus_tag="ACHLIF_004061"
     CDS             3926..4987
                     /gene="ybgO"
                     /locus_tag="ACHLIF_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9607969.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5139..6185
                     /gene="abrB"
                     /locus_tag="ACHLIF_004062"
     CDS             5139..6185
                     /gene="abrB"
                     /locus_tag="ACHLIF_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO9607970.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6182..6973)
                     /gene="nei"
                     /locus_tag="ACHLIF_004063"
     CDS             complement(6182..6973)
                     /gene="nei"
                     /locus_tag="ACHLIF_004063"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO9607971.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7009..7743)
                     /gene="pxpA"
                     /locus_tag="ACHLIF_004064"
     CDS             complement(7009..7743)
                     /gene="pxpA"
                     /locus_tag="ACHLIF_004064"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO9607972.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7733..8665)
                     /gene="pxpC"
                     /locus_tag="ACHLIF_004065"
     CDS             complement(7733..8665)
                     /gene="pxpC"
                     /locus_tag="ACHLIF_004065"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO9607973.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGSGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8659..9315)
                     /gene="pxpB"
                     /locus_tag="ACHLIF_004066"
     CDS             complement(8659..9315)
                     /gene="pxpB"
                     /locus_tag="ACHLIF_004066"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO9607974.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9338..10081)
                     /gene="ybgI"
                     /locus_tag="ACHLIF_004067"
     CDS             complement(9338..10081)
                     /gene="ybgI"
                     /locus_tag="ACHLIF_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO9607975.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10352..11833
                     /gene="dtpD"
                     /locus_tag="ACHLIF_004068"
     CDS             10352..11833
                     /gene="dtpD"
                     /locus_tag="ACHLIF_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO9607976.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11875..13293)
                     /gene="phrB"
                     /locus_tag="ACHLIF_004069"
     CDS             complement(11875..13293)
                     /gene="phrB"
                     /locus_tag="ACHLIF_004069"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO9607977.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVKTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13290..13799)
                     /gene="ybgA"
                     /locus_tag="ACHLIF_004070"
     CDS             complement(13290..13799)
                     /gene="ybgA"
                     /locus_tag="ACHLIF_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO9607978.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13950..14501)
                     /locus_tag="ACHLIF_004071"
                     /pseudo
     CDS             complement(13950..14501)
                     /locus_tag="ACHLIF_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3507 a         3864 c         3511 g         3619 t
ORIGIN      
        1 ttaataaaat taataatatt tttaagaaaa actaaaaaca ttcgacaatc aaataagata
       61 ttcttgcacc gatatacttt tttcttttct gttaaaggac tccgttatgt ttaaaggaca
      121 aaaaacattg gccgcactgg ccgtatctct gctgttcaca gcacctgttt atgcggctga
      181 tgaaggatcc ggtgaaattc actttaaagg tgaagttatt gaagcaccgt gtgaaataca
      241 tcaggatgat attgataaag aggttgaact cggtcaggtg accaccagcc acattaatca
      301 gtcacatcac agcgatgccg ttgctgtcga cctgcgctta gtcaactgtg atctggaaaa
      361 ctccagcaac ggttccggtg gcaagatttc taaagttgca gtcacctttg atagctcagc
      421 gaaaaccacc ggcgcagatc cgattctcaa caacaccagc acgggtgaag ccaccggcgt
      481 cggcgtacgt ttaatgaata aagatcaaag caatatcgtt ttaggcaccg ctactccaga
      541 tatcgacctg gctccgacct ccagcgaaca aacgctgaat ttctttgcct ggatggaaca
      601 aattgatcag gctacacctg taacgccagg cgcagttacg gcgaatgcaa cctacgtgct
      661 ggattataaa taagtattac tttggcggcg ctatcgccgc ctttcataaa atatttatca
      721 ggtatggaca cagtgaatat ttatcgactc tcttttgtat cctgcctggt cgtggcgatg
      781 ccttgcgcat tggcggtcga attcaacctt aatgttctcg ataaatcaat gcgcgaccgc
      841 attgatattt cattattaaa ggaaaaagga gtcattgctc ccggtgagta ttttgttagc
      901 gttgcggtaa ataataacca aatcagtaac gggcaaaaga ttaactggca caaaaatgac
      961 gataaaacca ttccgtgcat caatgattta ctggtcgata aatttggctt aaaacctgaa
     1021 gtccgtcagt cgttaccatt gataaatcag tgcgtcgatt ttagctcccg acctgaaatg
     1081 ctcttcaatt tcgatcaagc caatcagcaa ctaaatatca ccattccgca agcctggctg
     1141 gcgtggcact cagaaaactg gaccccaccc tccacatgga aagaaggtgt cgccggtgtc
     1201 ctgatggatt acaacttgtt tgccagcagc taccgcccac aggatggcag cagcagcact
     1261 aatcttaacg cctatggaac agccggaatc aacacggggg catggcgctt acgcagtgat
     1321 tatcagttga atcagactga tagcgatgat aaccatgaac agtcaggcga aatatcacgt
     1381 acctatcttt ttcgcccgtt accgcaatta ggttcaaaat taaccctcgg cgaaacggat
     1441 tttagttcca atattttcga cggtttttct tataccggcg cggcactggc aagtgacgat
     1501 cgaatgttgc catgggaact acgcggctac gccccacaaa ttagcggtat tgcacagacc
     1561 aatgccacgg tgacgatcag tcaatcaggc cgcgtcattt accagaaaaa agtcccacca
     1621 ggcccattta tcattgacga ccttaatcag tctgttcagg gcacactgga tgtcaaagtg
     1681 acggaagaag atggtcgggt gaacaatttc caggtttcgg cagcatcgac gcccttcctg
     1741 actcgtcagg gacaggttcg ctataaactg gccgcgggtc agccacggcc ctccatgtca
     1801 catcaaactg aaaatgaaac cttttttagc aatgatgttt cctgggggat gctgtcaaac
     1861 acctcgctgt acggcggcct gctgctttct ggtgatgact accattctgc cgcaataggt
     1921 atcgggcaaa atatgctgtg gcttggcgcg ctgtcgtttg atgtcacgtg ggccagtagt
     1981 cactttgata ctcagcagga cgagcggggc ttaagctacc gttttaatta cagcaaacaa
     2041 gtggatgcca ccaacagcac gatttccctc gccgcttatc gtttctccga tcgtcatttt
     2101 cacagctacg ccaactatct tgatcacaaa tacaacgaca gcgatgcgca ggacgaaaaa
     2161 cagacgatca gcttatctgt gggccaaccg attaccccac taaacctcaa tctttacgcc
     2221 aacctgctac atcaaacctg gtggaatgca gacgcctcca cgaccgccaa catcacggca
     2281 ggttttaatg ttgatattgg tgactggaga gatatctcta tttcgacgtc attcaataca
     2341 acccattacg aagataaaga tcgcgacaac cagatttacc tgtcgatttc gctccccttc
     2401 ggtaacggtg gtcgggttgg ctatgacatg caaaacagta gccacagcac cacacaccgc
     2461 atgtcgtgga acgatacgct ggatgaacgt aatagctggg gcatgtctgc cggactgcaa
     2521 tccgaccgtc cggacaatgg agcccaggtg agcggtaact atcagcatct gagttcagcg
     2581 ggtgagtggg atatttctgg tacctatgcc gccaatgatt acagttccgt cagcagcagc
     2641 tggagcggtt ctttcaccgc aacccaatat ggtgcagcgt ttcatcgccg cagctccacc
     2701 aatgaaccac gcctgatggt cagcaccgat ggcgtggcag atattccggt tcagggcaat
     2761 ctcgactaca ccaaccattt tggcattgcg gtggtgccgt tgatttccag ttaccagcct
     2821 tccaccgtgg cggtgaacat gaatgactta cccgacggcg taacagttgc agaaaacgtt
     2881 atcaaagaaa cgtggattga aggcgcgata ggttacaaat cactggcttc ccgttccggt
     2941 aaagacgtta acgtcatcat tcgcaacgcc agcggtcagt ttcctcccct cggagcggat
     3001 atccgccagg atgacagcgg cattagcgtg gggatggttg gcgaggaagg acatgcctgg
     3061 ttaagcggag tcgctgaaaa tcaaaagttt accgtggtct ggggtgatag ccagcattgc
     3121 tcgctccatc ttcctgaaca tatggaagac accgcaaatc gcctgatttt accttgtcat
     3181 taattaagga aatgaaaatg acatttatga aaggactacc ccttttgttg ttggttgcca
     3241 gtctgtgcag ccacgctgca ctacaacccg atcgcactcg tattgtgttt aacgctaatg
     3301 ataaagctac cagtctgcgg gtggataatc gtagcgataa actcccctac ctcgcttatt
     3361 cctggctcga aaatgaaaaa ggggagaaaa gcgatgatct tctggtcgcc ctgccgccga
     3421 tacaacgtct ggaaccgaaa gcaacaacgc aggtacggat agtaaagcaa gcctcgacca
     3481 caaaattacc gggcgatcgc gaaacgctat ttttctacaa tatgcgtgaa attcctcccg
     3541 ccccggaaaa aaacagcgac catgccgttc tgcaagtcgc cattcagagt cgtatcaagg
     3601 tgttctggcg accggctgca ttacgcaaga aagcaggaga aaaggtcgaa ctgcagttac
     3661 aggtcagcca gcagggcaac caactgacac tgaaaaatcc taccgcgtat tatctgacca
     3721 ttgcctacct gggacgaaat gaaaaaggcg ttctccccgg cttcaaaacc gtgatggttg
     3781 cgccttttag tacggtcaac acaaacatcg gaaattatag cggcagccag ttttatctcg
     3841 gttatatgga tgattacggc gcgttacgca tgacgacgct caactacagc ggacaatgcc
     3901 atttacaagc cgtggaggcg aagaaatgag tgctggcaag ggagtgttgc tcgtcatctg
     3961 tctgttattt ctgccgttga agtctgcgct tgccctgaac tgctactttg gttcatccgg
     4021 tggctcggta gaaaaatcag aagcgattca accttttgct gtaccaggca atgccaaact
     4081 cggcgataag atttgggaat ctgacgatat taaaattccc gtctattgtg acaacaatac
     4141 caacggcaat tttgaaagtg agcatgttta tgcctgggta aacccctacc cgggggtgca
     4201 ggatcgctat tatcaactcg gcgtaactta caatggcgtt gattatgacg ccaaccaggg
     4261 aaaaagccgc atcgacacta accagtgtat tgacagtaag aacattgaca tttatacccc
     4321 tgagcagatt atcgcgatgg gatggcagaa caaaatttgc tcgggtgatc ccgctaatat
     4381 tcacatgtca cgtacctttc tcgctcgcat gcggttatac gtcaaaatac gagaaatgcc
     4441 gccgcatgat tatcaaagta cgcttagcga ctatatcgtc gtgcaatttg acggtgccgg
     4501 tagcgttaac gaagatccta ctgcccaaaa cctgaaatat catattaccg gtctggaaaa
     4561 cattcgcgtg ctggattgca gcgtcaattt ttccatttca ccggaaacac aggtgattga
     4621 ttttggtaaa tttaatttgc tggatattcg tcgccacaca atgtcgaaaa cattcagtat
     4681 taagacgaca aaatcacaaa atgaccaatg caccgacgga tttaaagtca gttcctcctt
     4741 ttataccgaa gaaacgttgg ttgaagaaga taaagcattg ttgattggta atggtctgaa
     4801 gctacgtttg ctggatgaaa acgcctcgcc ttataccttc aataaatatg ctgaatacgc
     4861 cgatttcacc agcgacatgt tggtctacga aaaaacctat acggctgaac tttcgtccat
     4921 tgcaggtacc cccatcgagg ctgggccctt cgatacagtg gtgcttttta aaattaacta
     4981 taactgattt gccatgatgc cctttgcagc cggaaagggc atctctttca gaaaattgct
     5041 gattttcccc ttttccttga ttgctgttat tttttcctgg cccggctttc tttatgctgt
     5101 atcgatacac caaagagaaa ataatgaggg agcgaaggat gccagttttg cagtggggaa
     5161 tgttatgtgt gctatcactt ctcctttcta ttggtttcct cgcggtacat ctcccggcgg
     5221 cgctattact tgggccgatg atcgccggga tcatcttcag tatgcgcgga attactctgc
     5281 aacttccccg ctccgctttt ctcgccgcgc aggccattct tggctgcatg attgcgcaaa
     5341 acctcactgg ttcgattctc accacgctgg ccgccaactg gccgatcgtg cttgctattt
     5401 tactggtgac gctgctttcc agcgccatcg tgggttggtt attggtgcgc tatagctcac
     5461 tgcctggaaa taccggtgcc tggggttctt ctccaggcgg cgcagcggca atggtcgcta
     5521 tggcgcaaga ttacggcgca gatattcgcc tggtggcgtt tatgcaatat ctgcgagtgc
     5581 tgttcgtcgc gggggccgct gttctggtca cccgcatgat gctgggtgat aacgctgaag
     5641 cggttaacca gcaaatcgtc tggttcccgc cagtgagcat taatctcctg cttacgattt
     5701 tgctcgcagt cgttgccggt acggcgggat gcatgctgcg tcttccttcc ggcacgatgc
     5761 tcatcccgat gctggcgggc gcagtgctcc agtctggtca gctcatcacc atcgaactcc
     5821 cggaatggct gcttgcgatg gcgtatatgg caattggctg gcggattggt cttggtttcg
     5881 ataaacaaat cttactgcgg gcactacgcc cgctaccgca aatcctgctg tcgatttttg
     5941 ctctgttggc tatttgtgcg ggtatggcgt gggggctgac ccggtttatg catattgatt
     6001 ttatgactgc ctacctcgcc accagccccg gcgggcttga taccgtggcg gtcatcgccg
     6061 cagggagcaa tgccgatatg gcgcttatta tggcgatgca aaccctgcgc ctgttcagta
     6121 ttttgctgac gggacctgcc attgcacggt ttatttcaac ctatgcgccg aagcggtcgg
     6181 cttagtgctg gcagccaggg caccagtaaa acgggcgaga tgacagcgtg gttttctcaa
     6241 tgatgctgcc acaacgttcg cacggttcgc catctcgatg aaaaacctta aagcgaaaca
     6301 gcgccccatg atgcttattc tcatccacct gcccccgcgt agcgtaagaa aatcgaggaa
     6361 tctccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc attgagatct ttcgctttat
     6421 gatttccagt caacccaacc tgccagagga tctccacccg caaataattg ccaagtcctg
     6481 ccagaaacgc ctgctcgagc agtaatcctg caaactgacg gttacgaaag cgcggcgaca
     6541 atacccgttc tttcaccacc tccggcgtca gattcggatc cagcacatcg ggaccaacgc
     6601 gttgtaaaaa cggatgcgtg gtcagttgtt ccggggtcaa catctcaata tccgaggcgc
     6661 tataaagcag aatggttttg tcagccgttt gcagttttac ccgcaatact cgcgtggtct
     6721 gcggctcttc gccggtatca accacgcgcc agacgccgta aagctgatta tggctgtaga
     6781 gcgttaattc gttggaaaaa tgagttaaca acgccttacc acgcgtttcc acatgggtaa
     6841 cgtgttgacc gataagttgt gattgataag tttttaactg cgggaaggca aaccagacat
     6901 cagttagtgg tttgcctttg atcgccgcct ccaggttatc cgctgcacgg cggatctccg
     6961 ggccttcagg catggtgata tccttttttc attctttatt aagcggggtt atgctgcgac
     7021 aacaatcccc tttttggcaa atgtagctcg taatcggcgg gcgaacgcca gagcgtgctc
     7081 gccgtcgcca tgcaggcaga ccgtttgcgc cgcgaccgtt gcccattcgc cggtgatact
     7141 tttgactctg ccgtgttgca ccatctccag cgtttgcgcc agcgcctgtt gttcgttttc
     7201 aatcaacgcg cctggctgac ttcgcggcac cagcgagccg tcagcctgat aaccgcgatc
     7261 ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa tcagctcgct
     7321 tcctgccagc ccaacgagaa tcaatgccgg atcgcaagcg tataccgctc tggcgatggc
     7381 gtctgccagt tgtgcttctt tcgccgcctg gttgtacaac atgccgtgcg gtttgacatg
     7441 acgcattacg ccgccttgcg cacgggcaat cgttgccagc gcgccaattt gatacagcgt
     7501 ctgggcgtaa acggtttctg gcggcaactg catggcgctg cgaccaaaat tttccctgtc
     7561 gggaaaactc gggtgagcgc caattgcaac accatttttt attgcttcac gcacgcaagc
     7621 ctgcatggtt tgcgcatcgc ctgcatgaaa tccacaggca atattggcag aggaaaccag
     7681 cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca ggtcaatttt
     7741 cattgtgcag ccgccacgct aattgttcaa aataacgttg ctgatcttgc cgcgctttca
     7801 gtgcctcttc cagtgaacac tggacaaaat gaatcggctg accgagcgga atttgcgcaa
     7861 gatggtacat atcagcctca atgatgcagg caatacgcgg gtaaccaccg gtggtctgtg
     7921 cgtcgttcat caacacaatc ggctgcccgt tatgtggcac ctgcaccacg cccggtaaca
     7981 aaccgtgaga taacagttcg cgatcggtgg tgcgttttaa aatttgcccc tgtaagcgat
     8041 agcccatgcg gttactttgc gagctaagct gccagggcga acgccagaat gcatcctgcg
     8101 aggcgcgatc gaactcatga tattccggcc ccggcaaggc gcgaatgcgg ttgccccaca
     8161 gcagctgttt aacgccctgc gcttccataa aatcacgctt cgctttgcca atcgggagtc
     8221 ggtcaccatc cttcagtaaa cggccttcca gcccgccact ccccactttg agatcggtgc
     8281 tgcatgaccc cattaccggc ggaacatcaa taccacccgc gaccgccaga taactgcgca
     8341 tcccgtgctg cgggcgttta agcgttaaac gctggcctgc tttcatcggc aatcgccagc
     8401 cggtccagac ggcattatca tccagccgcg cttcgcaacc ggcacccgtc agagcaaacc
     8461 acccatcagt ttcgaactca acagttaact gaccgagcgt gatctccagc gcgggggcat
     8521 tggcgtcatt acccaccagt aggttagcaa tgcgtaacgc gggcatatcc agtgcgccgc
     8581 agtggctgat acccgactgg cgaaaaccgt gacgaccgcc atcctgcaca gtggtataca
     8641 tgcccgcacg aataatcttc agcatactcc ctccttctgc ggtacaaagc gcacgctgtc
     8701 tcccggacgt aataagatgg gttcgtcacg cgccggatca aacaggctga gtgaggtatg
     8761 accaatcaac tgccagccac ccggcgttgc cagcggataa acaccagtct gcggcccgcc
     8821 gatcccgaca gaacctgccg gaacgagtaa gcgcggttca gcgcgccgtg gcgtgtgtag
     8881 ttgttccggc aaactcccga gatacgggaa gcccggttga aaacctaaaa accagaccac
     8941 gtattccacg gaggagtgca attcaacaac ctgtttttcg ctcaacccgc aatgcgccgc
     9001 gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc accggaattt caataaagcg
     9061 agactcaggt tccagggcct cgctctcctc ccaccagcgt tgcaaacgct ctatggcatc
     9121 cagcgccagc gactcaggat tacgcaaaat caccgtaata ttgttcatgc cgggaatggc
     9181 ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt cgccagatcc gtttctggct
     9241 agccagcgtc accggcggtt ccagttccag cactaccgcc gtttcaccta tcagataaca
     9301 acgcgctcgt tgcactttcg atccctctga ttattcgtta tgcaggatta ggaatatcaa
     9361 taaaggtcac atcaagatcg gtattttcat tcagccactc gctcaatgcg cgaataccac
     9421 cacgttcagt ggcatggtga cctgcagcat aaaaatgcaa tccctgctcg cgggctgaat
     9481 gaatggtctg ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa cgcgcggcgc
     9541 tatcgataaa actttgcccg ccgcccgtgc accaggcgac gcgctggacc acctcaggtc
     9601 cggtatcgcc acaccataac ggcttacgtc ccagacgcgc ttcaatccag gaggctagtt
     9661 ccagtcccgg cacaggcatg gtcagctctc cccacggcac caacggctca atttcgccca
     9721 tgacagtgat ccccagtaac gccgccagct gtgcgttatt gcccagctca ggatgtgcgt
     9781 caagcggcaa atgccagcca tacaggttga tatcattcgc cagcaacgtt tttaaacggt
     9841 tacgcttcat tccgcgaatg accggagact ctcctttcca gaagtagcca tgatgcacaa
     9901 tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa agcctggctg gcggtgacac
     9961 cggtaacaat tttttgcacc gtctctttgc cttccacctg caaaccattc ggcgcgtaat
    10021 cactaatcgc cgcgctgttc agtttttcgt taatcagttg ttccagttcg gtgtttttca
    10081 tcttcgcgcc ttccgtattc tgatagctac accgttaaca tagcgttacc tcgcctgttc
    10141 gtcgatagcc aatcttttga catattaaga aacaaacccg acgccaaaag acgcaaattg
    10201 tttatatcat cacgcttcag ctaatgtata tttatgcaat cagacagcat attgcataaa
    10261 taatcagatc tgtatcaaag ttttaacttt aaggaaacac aagcacgccg cgcaactatt
    10321 acgtgacgtt gccgtcaatt gtggaattat tatgaataaa cacgcatcac aaccgcgcgc
    10381 tatttactat gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg gcatgcgtgc
    10441 cctgctgatt ctctatctca ccaatcaact aaaatacaac gatactcacg cctacgagtt
    10501 atttagcgcc tactgttcgc tggtgtatgt cacgccaatc ctcggtggct ttttggcgga
    10561 taaagttctc ggcaatcgca tggcggtgat gctgggggcg ttgttgatgg cgatcggtca
    10621 tgtggtgctg ggtgccagtg agatccatcc gtcattcctc tatctgtccc tggcgattat
    10681 cgtttgtggc tatggtctgt ttaaatccaa tgtcagttgc ctgctcggcg agctgtatga
    10741 gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg tatgcagcgg gtaacgtggg
    10801 gtctattatc gcacctatcg cctgtggtta cgcccaggaa gagtacagct gggcgatggg
    10861 ctttggcctg gcggcggtgg gtatgatcgc gggtctggtc attttcttat gtggcaatcg
    10921 tcatttcaca catacccgcg gcgttaataa aaaagtactg cgtgcaacaa actttctcct
    10981 gcctaactgg ggatggctgc tggttctgct ggtggcaacg cctgcgctga ttaccgtact
    11041 gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg actattattg gcctgggtgt
    11101 actggcaaaa atttatcgca aagcagaaaa ccagaaacag cgtaaggagc tggggctgat
    11161 tgtgacgctc accttcttca gtatgttgtt ctgggccttc gcacaacagg gcggcagctc
    11221 gattagcctt tatatcgacc gcttcgttaa ccgcgatatg tttggttata ccgttccgac
    11281 tgctatgttc cagtcgatta atgccttcgc agttatgctg tgcggtgtgt tcctggcgtg
    11341 ggtggttaaa gaaagcgtcg caggtaatcg taccgtgcgc atctggggaa aatttgctct
    11401 tggccttggc ctgatgagcg ccggattctg cattctgacc ttaagcgccc gctggtccgc
    11461 aatgtatggt cactcttctc tgccactgat ggtattaggc ctggcggtga tgggctttgc
    11521 ggaactgttt atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa ttccgggcgt
    11581 gaccggcgta ttaaccggca tctacatgct gctttctggc gcgatcgcga actatctggc
    11641 gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat gcttccgggg cgatcaacta
    11701 ctccatcaat gcatatattg aagtatttga tcaaattacc tggggcgcac tggcgtgtgt
    11761 aggactggta ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc gcgcgctggc
    11821 gctggagtca taataggcca gcaccatacc tgataaaacg ctcgctgcgt tttatcaggc
    11881 tcctttcctc gcttcttcgt acgctgccag cgttcgcagc cttgcctctt tgtgatcaac
    11941 tatcggttgc ggataatcca gcatcacacc tgctttctgc gcccacttcc acggctcatg
    12001 caccgctttc cctggtacat cgcgcagctc cggtaaccat cggcggataa actcaccttc
    12061 gcgatcaaat ttctcgccct gggttgtcgg gttgaaaata cgaaaatacg gcgctgcatc
    12121 agtacccgta gacgccgccc actgccagcc accgttattg gctgccaaat caccatcaat
    12181 cagctgcgac atgaaatatc gctcgccttc gcgccagtcg atcaacaaat ctttcaccag
    12241 aaaactggct gaaatcatcc gtagccggtt atgcatccag ccagtgctgt taagctgacg
    12301 catggcggca tcgacaatcg ggtatcccgt tttgccttcc tgccaggcct gtaaatgtgc
    12361 gggattgctc tgccactgca cacgatccgt ccaggcaata aacggacaat gtttacacaa
    12421 cgaggggtaa tacgtcatca gatgacggta gaactcgcgc cagatcagct cactaagcca
    12481 gacactaccg gccccaccgt ccagcgcctg cggctgttcc gccaacaagc gatgcaaaca
    12541 ctggcgaggc gataacccgc ccgttgccag gctggcggac aaacgactgg tgccttccac
    12601 tgccggaaaa tctcgttgtt gctcatattc tccggcaccg ttctggcaaa actggcgtaa
    12661 ttgcgcaatc gccgcttttt cttccaccgg gaaatgcgca gtatcgaaag actgacgagg
    12721 ataattcagc gtaatggatg gcgagggctc tatcgatccg ctactacgaa cttttggcgc
    12781 agcgacgcac tccggcatcc cttcccgcag ccgtttcagc caggcattct taaaaggcgt
    12841 aaagactttg tacatctcgt gattaccggt cttcaccgcg ccaggcggca ggatcacgct
    12901 gtcatcaaat ccttcacaca ccacgttacg cagcgctctt tcaacttcca catcccgcgc
    12961 ccgctcattc acttcatact gatagttata aaacaggtgg gtaacgctgt tttccgcgca
    13021 cacctgttta actatttcga cactggcgac aaagtcatcc acttcacgga acaataaagg
    13081 aatacctttt tccgcaagcg ctatttgtag cccattcagt tgagcattga taagttcagc
    13141 ctgacgcggc gacatgttat gcgtcgccca ctggcgtggt gtagcgatat acaacgccag
    13201 cacgcgtgca gacgaattgc ggcaggcggc agccagtgcg agattatcgt gcagacgtaa
    13261 atcctggcga aaccagacca gatgggtagt cataaaactc ctgaatggcg caagttaatc
    13321 acacgcggcc aaagttcgaa ataacgctgc cctgaaagcc aggcgtcagg ataaagcgcc
    13381 atatagtgtt tgatacgcat cagcggcgca agaagtggtt gctcgccacg gcgataactg
    13441 tcgataagcg ccgccaactg ctggcgttct gtggaatcaa tatgcgggcg aaaataaccc
    13501 tgaacgtgca tcaaaacgtt ggtgtgatcg cgcgggttgg cgggatgaga aagcaaaaca
    13561 attacgcgtt ggtagtactg gttgtaaaaa tcatccagat tttgccactg gtgaatatca
    13621 gccacgaacg ggccaagttt gcggtactcc ggctgagaat gcgccagaaa gacgagttta
    13681 tagcggctgt gataatcgag aagcgccccg cgcgttaagc catgttcttt cagtcgatgt
    13741 aactcatgaa gggcgaagat acggattagg gtgctgtcat caaatcgttg ctgattcatt
    13801 actgctcctg aacggtttac aggagtaagt gtagacgttg agaaatgata tttgtgaagt
    13861 taatcctgtt gtagcaggac aaatttacga aagcctgctc cccgcaagga ctgacgtcag
    13921 gtagtttcta accatagctg ccctttctat tcaaacgtga aatcaataag ctcatcaggg
    13981 acatcgcaaa caatatgaag acgggtttct tctctgccgt gactcttttc actaattgcg
    14041 taactgtcat gcttgggatt atttaattct ttcagcggaa atttttcctc gaaggcttta
    14101 tttagccgcc cctggtttcc ttttacagcg aataaataat cacctccctg tttttgtatc
    14161 ttctctgcaa tatctttctg gcaacccatc gcatcggttg tgatgatttt tcctttaata
    14221 tccagcatgt taagaagttc tgggatagct gtgatctcat tggatttctt atccgtcttg
    14281 atctgcccga tgaccagact gtgcattgtt gagaacgcac taatgacatg aatcgctccc
    14341 ctgcggcgac tcttgtcata agagtgccgg agcgtttttc catcaattgc aatgacgtct
    14401 ttatcatttg atgaatggca atcacgcatc cagttaataa agcactcgtg aaattttgca
    14461 ggactgatac aggatacaac tctggcaata gtatcgtgaa c
//
LOCUS       DAZGOB010000131        14289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGOB010000131 DAZGOB010000000
VERSION     DAZGOB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.131"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            220..315
                     /locus_tag="ACHLIF_004072"
                     /pseudo
     CDS             220..315
                     /locus_tag="ACHLIF_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            339..4853
                     /locus_tag="ACHLIF_004073"
     CDS             339..4853
                     /locus_tag="ACHLIF_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO9607979.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            4854..6503
                     /gene="yfaQ"
                     /locus_tag="ACHLIF_004074"
     CDS             4854..6503
                     /gene="yfaQ"
                     /locus_tag="ACHLIF_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO9607980.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKLGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6508..7284
                     /gene="yfaP"
                     /locus_tag="ACHLIF_004075"
     CDS             6508..7284
                     /gene="yfaP"
                     /locus_tag="ACHLIF_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO9607981.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            7559..10408
                     /gene="rcsC"
                     /locus_tag="ACHLIF_004076"
     CDS             7559..10408
                     /gene="rcsC"
                     /locus_tag="ACHLIF_004076"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO9607982.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(10494..11144)
                     /gene="rcsB"
                     /locus_tag="ACHLIF_004077"
     CDS             complement(10494..11144)
                     /gene="rcsB"
                     /locus_tag="ACHLIF_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO9607983.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(11161..13833)
                     /gene="rcsD"
                     /locus_tag="ACHLIF_004078"
     CDS             complement(11161..13833)
                     /gene="rcsD"
                     /locus_tag="ACHLIF_004078"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO9607984.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         3464 a         3476 c         3822 g         3527 t
ORIGIN      
        1 gcagctttat gctcttctga ttggctgtat gtttgatgac aaaaaattag cgcaagaaga
       61 aaaaattacc ttgcgctaat gctctgtctc aggtcactaa tcacacctaa ctcattgatt
      121 aattgtgact ctaaatgtag tgttttgtcg cgttttgcag tatgttattt aatctattat
      181 atgaataaca taatgatttt taatgtattt ttgtaattta ctctccttct gacatggaag
      241 gacacccgat ggatacccaa cgattccaat cccaatttca ttggcattta tcgtttaaat
      301 tttctggcgc gatagcagcg tgcttgtccc tgtctcttgt gggaacaggg cttgctaatg
      361 ctgatgattc gcttccttcc agtaactatg cgccgcccgc cgggggaaca ttctttttgc
      421 ttgctgacag cagttttagc agcagtgaag aggcgaaagt gcgactggaa gcgccggggc
      481 gtgattatcg gcgctatcag atggaagagt acggcggcgt ggacgttcgc ctgtatcgta
      541 ttcctgaccc gatggcattt ttgcgccagc agaaaaacct gcatcgcatt gtggtgcaac
      601 cgcaatatct gggcgacggg ctgaacaata cgctgacctg gctgtgggat aactggtacg
      661 gcaaatctcg ccgcgtaatg cagcgtactt tctcttctca gtcacggcag aatgtgactc
      721 aggcattacc cgaattacat ctcggcaatg ccattattaa accttcccgt tatgtacaga
      781 acaaccagtt ttccccgctg aaaaaatatc ccttggtgga acagttccgt tatccactat
      841 ggcaggctaa accggtcgag ccgcagcaag gggtaaaact ggaaggcgca tccagcaatt
      901 tcatctcgcc gcagccgggt aacatttata ttcctctcgg ccaacaagag ccgggactgt
      961 acctcgtcga ggcgatggtt ggtgggtatc gggcgacgac ggtggtgttt gtttccgata
     1021 ccgtggcgct tagcaaagtg tcaggcaacg agcttctggt gtggaccgcg ggtaaaaaac
     1081 agggtgaagc gaagcccggc tcagagatct tgtggactga cggtcttggc gtgatgaccc
     1141 gcggtgtgac cgatgacagc ggtaccttgc agttacaaca tatatcgcca gaacgttcat
     1201 acattctggg taaggatgct gaaggcggtg tttttgtctc cgagaacttc ttctacgaaa
     1261 gcgaaatcta caacacccgc ttgtatattt ttaccgatcg cccgctatat cgcgcaggcg
     1321 atcgtgtcga tgttaaagtg atgggccgcg agttccacga tccgttgcat tcatccccca
     1381 tcgtcagcgc cccggcgaag ctttcggtgc tggacgctaa cggcagtctg ttgcaaaccg
     1441 tcaatgtcac gctggatgcg cgcaatggcg ggcagggaag tttccgcctg ccagaaaatg
     1501 ccgtagccgg aggttatgag ttacgtcttg cttaccgcaa tcaggtctat agcagcagtt
     1561 ttcgcgtggc aaactacatc aagccacatt tcgagattgg tttagctctc gacaaaaaag
     1621 agttcaaaac tggcgaagcg gtcagcggca aactgcaact gctttacccg gatggtgagc
     1681 cggtaaaaga tgcccgcgtg cagttaagtt tgcgcgctca gcaattatca atggtcggta
     1741 acgatttgcg ttatgccgga cgttttcccg tgtcgctgga aggcagcgaa acggtgtccg
     1801 acgccagcgg tcatgtggcg ttaaatctcc ccgccgccga taaaccgagc cgctatttgt
     1861 taaccgtctc cgccagtgac ggcgcggcgt atcgcgtcac caccaccaaa gagatcctca
     1921 ttgaacgcgg cctggcgcat tactctttaa gtaccgccgc acaatacagt aatagcggcg
     1981 agtcggttgt gttccgttat gccgcgctgg aatcttcaaa acaggttcct gttacgtatg
     2041 aatggttgcg tctcgaagac cgcacgagcc atagcggaga gctaccgtca ggcggcaaat
     2101 cctttaccgt caatttcgct aaacctggca actacaatct gacattacgc gataaagacg
     2161 gcttaattct cgctgggtta agtcatgccg tcagcggtaa gggcagcacg gcgcatactg
     2221 gtacggtaga tatcgtggcg gataaaacgc tgtaccagcc aggcgaaact gcgaagatgc
     2281 tgattacctt tccggagcca attgatgaag cattattgac gctggaacgc gatcgcgtgg
     2341 aacagcagtc gctgctttcg catccggcaa actggctgac gttacaacgt ttaaacgata
     2401 cccagtatga agcccgggtt ccagtgagca attcctttgc gcctaacatc actttttcgg
     2461 tgctgtatac ccgtaatggt cagtacagtt ttcagaacgc cgggatcaaa gttgccgttc
     2521 ctcagcttga tatccgggtg aaaacggaca aaacccatta ccagcctggt gaactggtca
     2581 atgtcgaatt aacctcgtcg ctgaaaggta aacctgtttc tgcgcagcta acggtaggcg
     2641 tggtcgatga aatgatctac gcgctgcaac cagaaatcgc gccgaatatc ggcaaatttt
     2701 tctatccgct ggggcgtaac aatgtgcgta ccagctccag tctgtcgttt atcagctacg
     2761 accaggcgct ctccagcgag ccggttgcgc ctggcgcaac taaccgcagc gagcggcgag
     2821 taaaaatgct tgaacgtcca cggcgtgaag aggtggatac cgcggcatgg atgccgtcac
     2881 tcacaaccga taaacaaggc aaagcgtatt tcacgttcct gatgcctgat tcgttaaccc
     2941 gctggcgtat caccgcgcgt gggatgaacg gcgacgggct ggtcgggcag gggcgtgctt
     3001 atctgcgttc ggaaaaaaat ctctacatga agtggagtat gccaacggtg tatcgcgtgg
     3061 gcgacaaacc ggcggcagga ctgtttatct tcagtcagca ggataacgaa ccggtggcgc
     3121 tggtgactaa atttgcaggc gctgagatgc gccagacgct gacgctgcac aaaggggcga
     3181 attatatttc gctgacgcag aacattcagc aatctggctt gttaagtgca gaactgcaac
     3241 aaaatgggca agtgcaggac agcattagca caaaactgtc ttttgtggac aacagctggc
     3301 ccgttgaaca gcagaaaaat gtcatgctcg gcggtggcga gaacgcgctg atgttgcccg
     3361 agcaggcgag taatatccgg ctacaaagta gtgaaacgcc gcaggagatt ttccgcaaca
     3421 atcttgatgc gttagtcgat ggaccatggg gtggcgtgat caacaccggt agccgtctga
     3481 tcccgctcag tctcgcctgg cgttcgcttg ccgatcatca aagtgccgcc gctaacgaca
     3541 ttcgtcagat gattcaggat aaccgtctgc ggctgatgca actggcgggg cccggagcgc
     3601 gctttacctg gtggggtgaa gatggcaatg gtgacgcctt ccttacggca tgggcatggt
     3661 acgccgactg gcaggccagc caggcgctcg gcgtaacgca acaaccggaa tactggcagc
     3721 atatgctcga cagttatgcc gagcaggcgg ataacatgcc gttattgcat cgggcgctgg
     3781 tgctggcgtg ggcacaggag atgaatctgc cgtgcaaaac gttgttgaaa gggttggatg
     3841 aagctatcgc ccggcgcgga actaaagatg aagatttctc tgaggaagac atccgcgata
     3901 tcaatgacag cctgatcctc gatacaccgg aatctccact ggcagatgcg gtggcaaacg
     3961 tcttaaccat gacgttgctg aaaaaagcgc agttgaagtc cacggtgatg ccacaggttc
     4021 agcaatatgc gtgggataaa gcggcaaaca gcaatcagcc gctggcgcac acggttgtgc
     4081 tgctcaatag cggtggtgac gctacccaga cggccgctat tttaagtggt ttgaccgctg
     4141 agcaatccac tattgagcgc gcgctggcca tgaactggct ggcgaaatat atggcgacaa
     4201 tgcctccagt tgttttgcct gcgcctgcgg gcgcatgggc taaacataag ttaactggag
     4261 ggggcgaaga ctggcgttgg gttggtcagg gcgtgccgga cattctctct tttggtgacg
     4321 aattatcgcc gcaaaatgtg caggtccgct ggcgtgagcc ggcaaaaatg gctcaacaaa
     4381 gtaacattcc ggtgaccgtt gaacgccagt tgtatcggct tatccctggt gaagaagaga
     4441 tgagctttat tctgcaaccg gtgaccagca atgagattga cagcgatgcg ctgtatctcg
     4501 atgaaattac gcttaccagc gagcaggatg cagttctgcg ctatggtcag gtggaagtac
     4561 cgctcccgcc gggagccgac gttgagcgca caacatgggg catttcagtc aataaaccca
     4621 acgccgcgaa acagcagggg caattgctgg aaaaagcgcg taatgaaatg ggcgaactgg
     4681 cctatatggt gccggtgaaa gaactgacgg gaacggtcac tttccgccat ttgctgcgct
     4741 tctcgcaaaa agggcaattc gttctgcctc ctgctcgtta tgtgcgttcc tatgcacctg
     4801 cgcagcaaag tgttgcggca gggagtgaat ggaccgggat gcaggtgaaa taagtgaact
     4861 ggcgcagaat cgtctggcta ttggcgttgg ttactctgcc aacgcttgcg gaggagacgc
     4921 cgttacaact ggtgctgcgc ggtgcgcagc acgatcaact gtatcagctg tcgtcatcag
     4981 gcgtgaccaa agtctcagca ttacctgact cgctcaccac gccgttaggg agtttgtgga
     5041 aactgtatgt ttacgcctgg ctggaggata cccatcaacc ggagcagccc taccagtgtc
     5101 gcggaaactc cccggaagag gtttattgtt gtcaggctgg ggagagcata acgcgcgata
     5161 ccgcgctggt acgttcctgt gggctctatt ttgcgccgca gcggttgcat attggcgcag
     5221 atgtgtgggg gcaatactgg caacagcgtc aggcacctgc gtggttagcg tctcttacaa
     5281 cgctgaagcc agaaacctcg gtaacggtta aatcgctgct cgattcatta gcgacgttac
     5341 ctgcgcaaaa caaagctcag gaagtcttac tggatgtggt gctggacgag gcgaaaatcg
     5401 gtgtcgcatc catgctcggt agtcgggtac gggtgaaaac ctggagctgg tttgccgatg
     5461 ataaacagga aatccgccag ggtggttttg ctggctggtt aaccgatggt acaccgctgt
     5521 gggttactgg cagtggcacc agcaaaacgg tactaacccg ttatgcgacg gtgcttaatc
     5581 gggttttgcc agtgccgacg caggttgcca gtgggcaatg tgtagaggtg gagctgtttg
     5641 cccgctatcc gctgaagaaa atcacagcgg aaaagagtac gacggcagta aaactcggcg
     5701 tacttaatgg gcgctatcgt gtgacgttta ctaatggtaa ccacattacg tttgtcagcc
     5761 acggtgagac gaccttactg agcgaaaagg gcaaactgaa attgcagtcg catctcgatc
     5821 gtgaagagta cgttgccaga gttctggatc gcgaggcgaa aagtacgccc cctgaggcag
     5881 cgaaagccat gaccgtggcg attcgcactt ttttacagca aaacgccaat cgggaaggcg
     5941 attgcctgac catccccgac agcagcgcca cgcagcgtgt ttctgcttcg ccagcgacca
     6001 cgggggcgcg aacgatgacc gcctggacgc aggatctcat ctatgcaggc gatccggttc
     6061 attatcatgg cagtcgagct accgaaggga cactttcctg gcggcaagcc acggcccagg
     6121 cggggcaggg agagcgttac gaccagatac tcgcgtttgc ttatcccgac aacagcctta
     6181 gccgctgggg agcgccacgc tcaacctgcc agttattacc caaagcgaaa gcctggctgg
     6241 cgaaaaaaat gccgcagtgg cggcgtatat tacaaggcga gacggggtac aacgaaccag
     6301 acgtgtttgc ggtctgtcgt ttagtctcag gtttccccta taccgatcgg cagcagaaac
     6361 ggctgtttat tcgtaacttc tttacgcttc aggatcggct ggatttaacc catgagtatc
     6421 tgcacctggc ctttgatggt tatcccaccg ggcttgatga gaactatatc gaaacgctga
     6481 cccgacaatt attaatggac taatgctatg cgaaagattt ttcttccgtt gttactggtg
     6541 gcactttcgc ctgtcgctca cagtgaaggt gtgcaggaag tcgagattga cgcaccgctc
     6601 tctggctggc atccggcaga gggcgaagat gccagttttt cacaatcgat caattacccg
     6661 gcgtcgtccg tcaacatggc agatgatcaa aatatctcag cgcagatccg cggcaaaata
     6721 aagaattatg ctgcggcggg taaagttcag cagggtcggc tggtggtcaa cggtgccagt
     6781 atgccgcaga gaattgaatc cgatggttca tttgcacgcc cttatatttt cactgaaggc
     6841 agcaacagcg tgcaggtcat cagcccggat gggcaaagcc ggcaaaaaat gcagttttac
     6901 tcaacgccgg gtacaggaac gattcgtgca cgtttacggc tggttctctc gtgggatacg
     6961 gacaataccg atctcgatct ccatgttgtc acgcccgatg gcgaacacgc ctggtacggt
     7021 aacaccgtgc tgaaaaacag tggtgcactg gatatggatg tcacgacggg gtacggaccc
     7081 gagattttcg ccatgccagc accagttcac gggcgctatc aggtgtatat caactactat
     7141 ggtgggcgca gcgaaacgga attgacgacc gcccaactga cgctgatcac cgatgaagga
     7201 tcggtcaatg agaaacagga aacatttatt gtaccgatgc gtaatgctgg cgagctgacg
     7261 ctggtgaaaa gttttgactg gtgatccatc ccttcacaca acctggctct ctcttttttc
     7321 agataagact gctgggcgat ctgccgacgc caggatataa ccattaacaa gcgtcggcag
     7381 attggtcatt tcttattttt cgtactgaga actgtccatc tactccggct ttcctggtta
     7441 aggcaatggt ttctaagcga tacgcattat tgcaatggtg acagaacatt gcggtaacac
     7501 gcttttaccg ctaccttaac cacactccat cggtcacctg aggcggagct tcgccccttt
     7561 gaaatacctt gcttcttttc gtacaaccct gaaagcctcg cgctacatgt tcagagcatt
     7621 ggcgttagtg ctctggctgt tgattgcttt ttcatccgtt ttttacatcg ttaatgcgtt
     7681 acatcagcga gaatcggaaa ttcgtcagga atttaatctg agttccgatc aggctcagcg
     7741 ctttattcaa cgcacctctg atgtgatgaa agagctgaag tacatcgccg aaaatcgctt
     7801 atcggcagaa aacggtgtgc tttccccgcg tggacgagaa acgcaggcgg atgtgcctgc
     7861 gtttgaaccg ctgtttgccg actccgattg ttccgcaatg agtaacacct ggcgaggttc
     7921 tctggagtca ttggcgtggt ttatgcgcta ctggcgcgat aatttttctg cggcttacga
     7981 tctcaaccgg gtatttttaa tcggcagtga taacctctgc atggccaatt tcggtctgcg
     8041 tgatatgcca gtggaacgcg ataccgcgtt gaaagcgttg catgaacgca tcaataaata
     8101 tcgaaatgca ccacaagatg atagcggcag taacctctac tggatcagcg aaggtccgcg
     8161 ccctggcgtc gggtattttt acgcgttgac gccagtttat ctggcgaacc ggttgcaggc
     8221 gcttttgggt gtcgagcaga ccatccggat ggaaaacttt ttcttaccgg gtacgttgcc
     8281 gatgggggtt accattcttg atgaaaatgg tcataccctg atttcgctta ccggaccaga
     8341 aagtaaaatt aagggcgatc ctcgctggat gcaggaacgc tcctggtttg gctatacgga
     8401 agggttccgg gagctggtgc tgaagaaaaa tctgccaccc tcatcgctaa gcatcgtgta
     8461 ttcggtgccg gttgataagg tgctggaacg cattcgcatg ttgatcctta acgcaatttt
     8521 gctgaatgtg cttgccggag ctgcattgtt tactctcgca cggatgtacg agcgacgtat
     8581 tttcattccg gcggaaagcg acgccctgcg actggaagaa catgagcagt tcaatcgcaa
     8641 gattgtcgcc tccgcgccag tgggtatctg cattttgcgt accgctgatg gcgtcaatat
     8701 tttaagtaac gaactggcgc atacctatct caatatgctt acgcatgagg accgccaacg
     8761 actgacgcaa attatctgtg ggcagcaggt caattttgtt gatgtcctga ccagcaacaa
     8821 taccaatctg caaatcagct tcgtccattc gcgctatcgt aatgaaaacg tggccatttg
     8881 tgtgctggtg gatgtttctt cgcgcgtgaa gatggaagag tcgttgcagg agatggcaca
     8941 agcagcggaa caggcgagcc agtcaaaatc gatgttcctt gccaccgtca gtcatgagct
     9001 gcgaacgccg ctgtatggca ttatcggtaa cctggatctg ttgcaaacca aagagttacc
     9061 gaaaggcgtc gatcggctgg tgacggcaat gaacaactct tccagcctgt tgttgaaaat
     9121 tatcagcgat attctcgatt tctcgaagat tgaatcggaa cagttgaaga tcgaaccgcg
     9181 tgagttttca ccgcgtgaag tgatgaacca catcaccgcc aactatttac cgctggtggt
     9241 acgcaagcag ttaggcttgt actgctttat tgaaccggat gtgccagtgg ccttaaatgg
     9301 cgacccgatg cgtttacagc aggtcatctc caacctgctg agtaacgcca taaaattcac
     9361 cgataccggc tgtatagttt tgcatgttcg cgctgatggc gattatctct ctatccgtgt
     9421 tcgcgatacc ggcgtgggca ttccggcgaa agaagtggtg cgcttgtttg atcccttctt
     9481 ccaggtcgga acgggcgtac agcgtaactt ccaggggacc ggtctgggtc tggcgatttg
     9541 tgaaaaactg atcagcatga tggacggcga tatctcggta gattcagaac cgggaatggg
     9601 cagccagttt accgtgcgta ttccgttgta cggcgctcag tacccgcaga aaaaaggcgt
     9661 ggaagggttg agtggtaaac gctgctggct ggcggtccgc aatgcgtcgc tctgtcagtt
     9721 cctggaaacc agtttgcagc gcagcggcat cgtcgttaca acatacgaag ggcaggaacc
     9781 gactcccgaa gatgtgttaa tcactgacga ggtagtgagt aaaaaatggc agggtagagc
     9841 ggtagtgacc ttctgtcgtc gccatattgg tattccgctg gagaaagcgc caggggagtg
     9901 ggtacacagt gtggctgctc cgcatgagct accggcattg ttggcgcgta tttatttgat
     9961 cgagatggag agcgacgatc ctgctaacgc tctgccgtcg acggacaaag cggtcagcga
    10021 taacgacgat atgatgattc tggtcgtgga tgatcatccg attaaccgac gtctgctggc
    10081 agatcagttg ggatcgttgg gctatcaatg taaaaccgcg aatgatggcg tcgatgcgct
    10141 taatgtactt agcaagaatc atattgatat cgtgcttagc gacgtcaaca tgccaaatat
    10201 ggatggttac cgcttgacgc aacgcattcg tcagttggga ctgacgttgc cggtaatcgg
    10261 agtaactgct aatgcgttgg ctgaagagaa gcagcggtgt ctggagtccg gtatggacag
    10321 ctgcctgtcg aagccggtaa cgctggatgt gataaaacag acgctgacgg tatatgccga
    10381 gagggtcagg aaatcgcggg aatcgtagga cggataaggc gtttacgccg catccggcat
    10441 tcagtgcaaa tgccagataa gacactaacg cgtcttatct ggcctacagg tgattagtct
    10501 ttatctgccg gacttaacgt cacagaagag agataattca gcagggcgat atcgttctcg
    10561 acacccagct tcatcatcgc agatttcttc tggctactga tggttttaat actgcggttc
    10621 agctttttag cgatctcggt caccaggaag ccttccgcaa acaggcgcag aacttcactc
    10681 tcttttggcg agagacgctt gtcaccgtaa ccaccagcac tgattttttc caacaggcga
    10741 gaaacgcttt ccggggtaaa tttcttccct ttctgcagcg cggcgagagc tttcggcaga
    10801 tcggtcggtg caccttgttt cagcacgatc ccttcgatat ccagatccaa taccgcacta
    10861 agaatcgccg ggttgttgtt catggtcaga acaataatcg acaggcttgg gaaatggcgc
    10921 ttgatgtact tgattaaggt aatgccatcg ccgtacttat cgccaggcat ggagagatcg
    10981 gtaatcaaca catgcgcatc cagtttcggc aggttgttga tcagtgctgt agagtcttca
    11041 aattcgccga caacattcac ccactcaatt tgctcaagtg atttgcgaat accgaacaag
    11101 actatcggat ggtcatcggc aataattacg ttcatattgt tcatgtaata ggctaccttg
    11161 ctacagcaag ctcttgacat aactgtcaat gtcgctgatg tatttttcta ttcctggaac
    11221 atccttctca cgaatcagat gttccagcgt ttcacataac tgcttgccgg gtaccagatt
    11281 tagcatggca aatacgcctt taagacgatg agccgtttgg gctaacgcag caaagtcact
    11341 ggttgctgct tcagtataca gcctcttaac atcatccggt actgtgtcta caaagagcgc
    11401 ataatagccg ctggcatgga gttgcgcatt ttcatctccg cccagaggcg attctgtcac
    11461 ctcttcctgc gccagttgca cttcaattaa ttgtaagacc gcttcctgca tagcgttgct
    11521 catattgaag ttgacgcaca attgaccagg cccaatttcc cgtacgccag actcatcatc
    11581 gcttaaaagc aagccagagg cagtaagatt agacggatta tccgttaaaa agatatcata
    11641 atcttgacta attaatcttt catcgggtgt gatacaggtt gcaccccaat tttctaactg
    11701 gcgagtgaca atattccgaa tttctgccga agtaacatcc accattacgc agacatcatc
    11761 cagtaaacgc tcttcttcct cttcaacttc cgggtcagcg gcgagcattt tgatatgcac
    11821 agagtagcgt gtaccaagcc catcccgcgt tttgatgttt aaatgaccgc ccagtttacg
    11881 cgccagttga tcgctcagcc agaatgccag cgggtccgcc ttgccatagc gatcgttttg
    11941 ggtctggttg ataaacggga agtgcaaatt atccatttca tgaatactta cgccttctcc
    12001 ggtgtccaga atgcggaatg tcaggcggtc ttcagaggac tcatcctgat caacctcaag
    12061 ggtgattttt cccaattgcg ttgaggtcac ggcatattgc atcagtagca gcaaaatacg
    12121 tcgtaaggca tcgcgatcgc cgcggcgcat atcgtgtgct ttcagatgat tgttaatcag
    12181 cagttgcaga cctttacgct tgatggcagg caacactgaa ggcacaactt catcaattaa
    12241 atcctgcacg gagaacagca ccgtctcact tttccagcta tcatccgcaa gcatgttcgc
    12301 taactgtatt tcatcaacca gccgcaccag cacatctgcc tgattcgcca gttgtttgct
    12361 ttccggggcg ttgagtttag ccgcgctctc cgccagggac tgtgcaggtt ctttcagcgc
    12421 atcgccaatg tttttcataa aggtcatccg gccctgctgg tttttctcat acagacgctg
    12481 cgcctgcttg agtttcttgt ttaccagcac ttcgcgatcc tgatcgcgaa taatgaaaat
    12541 ttgtgtgcgc ggcgcgacct ggctgcggaa catgcggatc tcatacagct cgttattgat
    12601 cgtcgcctga ataatcccct gatgctgttc cgccatggtg gtgatgtttt gcagattcaa
    12661 atgcggcagc aaatgatcgg caattttgtt acttatgaca gtgcggttcg attcctgatc
    12721 gtgaaccagc aggccgagcg gcagcagtga gactatctct tcattaattg cccgtaaaat
    12781 gcgcaattcg ttattgaccg ccgtgctggg tagactttct gtactgcggc tggagaaatg
    12841 gcggaatgtg gtatagccaa ataacgccag cgccagcaaa ccgatgttca gcagcagtgg
    12901 cagcagaatg ttttgcaacg tatccagcaa taaggtgcca taaggaacct gccagaccaa
    12961 gcgcatatcg gtagagttga gtgccgagga gatttcaatc ttcgtactgt taaagtggat
    13021 actgacgcta tccgtccctt ctttctcatt atcattgttt cccgttgccg tcgcgtctgg
    13081 ctcaaggcgg aaactgtcca gcggcatacc cggtgggatc aaatcattaa tcggcagatc
    13141 aaaagccacg accgttgcca gatgtcctgg ctggttaaag gtagtacgca aggtaaagta
    13201 atgaccgttc tgccaggcca ggcggcgcat gttagaaaag ctttcgcgtt catcgagggc
    13261 gttggcctgt tgcaacatct ctgcacgacg tgaatcaaca atgtcgctga cggtcgattc
    13321 tttaaatccg gaggtgagat ctttaagggg tagggttgag atcagcacca gactgttatc
    13381 ctgaccattc aggtaataca tcgaccacgg tacattttct gcgccccaca atgtatccag
    13441 ataagtggac atccgctgag tcatctcaag cgttgagttg tcgtgagagc caaagattaa
    13501 cgcttccgtt ttgcggcgtg gtttctccag atagtagaca tcctgtttca ggcgcgtctc
    13561 ttgtaaacct tcgccggagg agggggaggt cgtcgcggca atgttgtcgt agatctgcca
    13621 ggtcacgtaa cgccaggtat cgacgcgctt ttggatagcg tgggtaatgt cgacaatctg
    13681 gtaactttta tctttcagcc atgcgttaac ggcgctttgt accattacac ccatcgtcac
    13741 cagtaacaca atgatcaaca gtaaaaagaa gcgggtaatg ctccccggta ggagtgaaaa
    13801 gcgggtcgtg gccgttgtct ctttctgacg cattcgtgtt tatgacctgt taaaacttcg
    13861 cgaagtgata gggcagtata aagggtacag tgtgaaattc cagactctta cctgattgat
    13921 gattctctat tctcccgtga tatttatcat cctgcattgg gggaaataga tgtacaagcg
    13981 ccattttgct gtacaaataa cctacaaaaa gcccaacgaa aataattgca aataaacaaa
    14041 gattgctgga aattatgctg atgttaatta tttgtgaaat agttaacaag cgttatagtt
    14101 tttctgtggt agcaaagaat aatgaaaagt gtgtaaagaa gggtaaaaaa aaccgaatgc
    14161 gaggcatccg gttgaaatag gggtaaacag acattcagaa atgaatgacg gtaataaata
    14221 aagttaatga tgatagcggg agttattcta gttgcgagtg aaggttttgt tttgacattc
    14281 agtgctgtc
//
LOCUS       DAZGOB010000132        14193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGOB010000132 DAZGOB010000000
VERSION     DAZGOB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.132"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..541
                     /gene="ykgH"
                     /locus_tag="ACHLIF_004079"
     CDS             <1..541
                     /gene="ykgH"
                     /locus_tag="ACHLIF_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO9607985.1"
                     /translation="NKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRI
                     TTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEI
                     QTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVI
                     SVMFCLVKKMVDEYQQNSG"
     gene            complement(787..1482)
                     /locus_tag="ACHLIF_004080"
     CDS             complement(787..1482)
                     /locus_tag="ACHLIF_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO9607986.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1475..2902)
                     /locus_tag="ACHLIF_004081"
     CDS             complement(1475..2902)
                     /locus_tag="ACHLIF_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO9607987.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2913..3632)
                     /locus_tag="ACHLIF_004082"
     CDS             complement(2913..3632)
                     /locus_tag="ACHLIF_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO9607988.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4159..5013)
                     /gene="rclR"
                     /locus_tag="ACHLIF_004083"
     CDS             complement(4159..5013)
                     /gene="rclR"
                     /locus_tag="ACHLIF_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO9607989.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5239..6564
                     /gene="rclA"
                     /locus_tag="ACHLIF_004084"
     CDS             5239..6564
                     /gene="rclA"
                     /locus_tag="ACHLIF_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO9607990.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6673..6909
                     /gene="rclB"
                     /locus_tag="ACHLIF_004085"
     CDS             6673..6909
                     /gene="rclB"
                     /locus_tag="ACHLIF_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO9607991.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6921..7514
                     /gene="rclC"
                     /locus_tag="ACHLIF_004086"
     CDS             6921..7514
                     /gene="rclC"
                     /locus_tag="ACHLIF_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO9607992.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7674..8543)
                     /locus_tag="ACHLIF_004087"
     CDS             complement(7674..8543)
                     /locus_tag="ACHLIF_004087"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO9607993.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            8792..9649
                     /locus_tag="ACHLIF_004088"
     CDS             8792..9649
                     /locus_tag="ACHLIF_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9607994.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(9770..14023)
                     /gene="fdeC"
                     /locus_tag="ACHLIF_004089"
     CDS             complement(9770..14023)
                     /gene="fdeC"
                     /locus_tag="ACHLIF_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO9607995.1"
                     /translation="MSHYKTGHKQPRFRYSVLTRCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSGQSTVSVAAD
                     RVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTKLTFTV
                     KDAYGNLVTGLKPDAPQFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     NADASTGQANLQVDTAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         3695 a         3576 c         3118 g         3804 t
ORIGIN      
        1 caataaagat aatataaaag tgagttacag cctaaaaata aaccagacaa caccaggcat
       61 acttgttaac tgtgatagca ataataactt tgcctgtcag accacaatga ctgaagatgt
      121 tatacagcga attactacat tttttcacac cagcccagat gtcaagaaca gagaaataaa
      181 gctggaatgg tcaggagata agagagattt accaactgct gaagcggaaa tatctcgcgt
      241 gcaggcgtct attatcaaat ggtatgcgtc agaataccat aatggcaggc aagttctcga
      301 tgagatacaa acaccttcag caattaacag tgagctttat acaaaaatga tatacctgac
      361 caggaactgg tcattgtatc cgaacggtga tggctgtgta actataagct caccagaaat
      421 aaaaaataaa taccctgctg ccatttgcct ggctctggga ttttttctaa gcattgtaat
      481 ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa taccaacaaa actctgggta
      541 gtaataaaca gcattctgat agagagtgcc tgtataaagc tgaagtgaca atgaccggga
      601 agggaacccg ttttcaggaa tggatgcctg tctttttcat tgtaaatatc ctggcattac
      661 taatgccagg atatttacag aagagcagca taaccatgac ggaacggggt taagtcaggt
      721 tgtccttaac gctattttca acctcgtatg ccttcttcag gtttatgttc agatctcata
      781 tctctctcag caatcctcaa taatcagata caccgctttc accggaccat gaaccccgac
      841 gactttgata agctcaatat ccgccgttga actagggccg ctaatgatgt taatgcagga
      901 cggcattcgt tcaccggcct gcgctttctg atgcaatttt tctgcgagtt gcgctacacg
      961 cggcaagata gtgcttttac gcaggataaa aagagaagat tccgggagca ggctcaatga
     1021 acgcccgcgc ccggcggcgg aaaaaagaac cacgcctccc gattcggtta aaccatattc
     1081 agcatacacg acacccactt tagcctgctc ggcctgcgag atattctcgg cacctttcgc
     1141 cggatcccaa acaacggcat tgcattcctg ctgcaaacgt tcactaatcc ccaattcctc
     1201 cagcctcgtg tcaccgctaa tcacgaccga ctgatctccc agctctttac acagacgtat
     1261 tgcagcttct gccgccttcg cctcgctggt cagctcacag cgcgtcaaca taacatcgct
     1321 ggcaaactga ataaacgcgt cacagcgctg ctgttggtta agttgggtaa gccgctcgtt
     1381 agcatagttg ttaagcggcg catcttctgc ttgcggttca agtcgcagcg ggcgacccag
     1441 tgcctgagca acgttattca aaaattcgcc tcgattatcc attctttttc tcctgcgcct
     1501 gatgtttctt aaaccaacta cggaaactct ctccgtcagc ttcaggaaga tcgcgtgctt
     1561 ccatccagtc gctaatcgcg ccaaatttga gtggtgtttt gccgccattg ataaaccagc
     1621 ttgccgcatg agcgccggcc atcatcccga ctttccacaa tcctggatga ctattggcat
     1681 aagcgaacat ttttatcgcc cgttgctctg cttttgcggt gatacctttt tcagccatca
     1741 cccgacgatg acgcaaaatc agttttgaca gcggaatacg caccggacac acgctgtcac
     1801 aagcagtgca taaagagcag gcgtagggta aatctttaaa atctttatag ccgccaagta
     1861 gcggagaaat caccgcacca attggccctg gataaataga gccatatcca tgaccgccaa
     1921 tatggcgata tgccggacaa gtattcatac aagccccgca gcgaatacag cgcagcacat
     1981 cccgaaattc agaggccagc acctcagaac gcccgttatc gacaataacc agatgaaact
     2041 cttcaggacc atcaacgtgc cccgcttcgc gcggtcctgt cagccaggtg ttgtatcccg
     2101 tcaaacgtgc accaacggca ctgcgcgcca gcatggtgat caatacatct acctcggcaa
     2161 acgtgggggc aatacgctcc attcccatca ctgcaatatg cgttttaggc agcgtggtac
     2221 acattcgcgc attaccttca ttggtcacca ggcataccga acctgtctct gccaccgcga
     2281 aattacagcc ggtaatacct atttcagcac tgaggaaatc ttcgcggatt ttttgccgga
     2341 tgaataaggt catcgcttca ggcgtttccg gcccctcata gcccagacgt tcgtgtagca
     2401 ctcgacggat ctgatggcga tctttatgaa ttgccgggac cacaacatga gatggcggat
     2461 cttgatccag ctggagaatg tattcaccca gatcggtttc aatcacctga atgccagcat
     2521 cctgcaacac atgattgaca ccaatctctt cggtcaccat cgatttagat ttcaccacct
     2581 tccgggcatt tttgcgttgg gcaacctgta aaatgtagcg agtggcgtct tctttagttt
     2641 ttgcaaaata gacgtgaccg ccgttttgcg tcactttttc tgagagctgg tacagataag
     2701 cgtcgagatt acttagaaca tgatcacgta tctgggcggc ccgatcgcgc cactcctccc
     2761 agtgccccaa ttcatcgacc attttttgcc gatttgcccc aatacgctgc tgcgcgtttg
     2821 ccaccgcttt gcgcatgatc ggatcttcaa tttgctgacg gatgcgtgtc ttaaaatctg
     2881 tattactggt tttgatcgac atctttatat cctcagcggc tcatcaacac ttcagcaata
     2941 tgcatcactt tgactttctg cccttcccgt tgtaatcgcc cactgatgtt cagcaggcaa
     3001 ctcacgtcag caccaattaa atactcaggg cggacttcca tcaggtgcac aaccttttct
     3061 ttcaccatct cgccggatat ttcggccatt ttgaccgaga acgtgccgcc aaatccgcag
     3121 caggtatcct gttcagcaaa ggtcaacagc tccagtccac gcacattttt cagcagcgta
     3181 agtggctcgt ccttcactcc cagcttacgg gccaggctac aagatgggtg atacacagct
     3241 ctcccttgca aactggcacc tacatcgact acccctaatt tattaacaat aaaagaggtg
     3301 agatcctgca tacgcgcggc aaccttttcg gcacgtgatg cccattcagg ttcatccgcc
     3361 agatacgtcg ggtaactttt tacggcatag gtgcaagagc cagccggtga aataatggga
     3421 tcgtcgttat cctccagtgc ggcgatcaga tttttcatcc ctggaatcgc ttctttgata
     3481 taaccgctat tgatcgcagg ctgaccgcag catccctgtt tctccgggaa atttacgcga
     3541 cagccgagtt tttccagtag cagcacggag tctcgtgcca ttcttgattt cagggcgtca
     3601 ccaatacagg tgacaaagaa attgacattc acaactaata ctccattact tcatgcccat
     3661 ttatgcccga tgtttccccc ccaccggggg atcaatcatc aataaatatg gcgttctttt
     3721 ttaattggct agcaacatac cacccctcat ttgtatgaca aatgataatc tttcatccaa
     3781 acttcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt atggggaaat
     3841 cgacgttcat ttttggcata agaaatccat ttaaaaaggc tacatccctt atacctattc
     3901 agagctactt attcaggggt tattgagata tacactgatg gtgtacaaaa aaatcaccga
     3961 caataatcac gtatcaagta atagtcgggg ttttatttaa ttgaagcaat ttagtttgca
     4021 tttttattta aaaactgagc tagagtccat cagaaaaaaa tatataaagc ccaaaccacc
     4081 gttggaaata gaaatagttg aaaggctcac aactcccgga atgcggatta tctttgccct
     4141 gatttctggc ggttttattc agggtgcaag ctgtctgacc ctttcccgat attctcccgg
     4201 agtacaacca aactcgcgga caaacgcctt gtgaaaagat gattcactgg catagcctac
     4261 tgactcagcg atcaccacaa cagggagcat ttcccgggaa aacatctggg ccgctatttg
     4321 tagacgcaac tttgttaata cagccagcgg cgtggttccg gaaacatcac ggaaaagctg
     4381 ggcaaaactt gcccgggaca tgtgagcgat gctggccagc gattcgacgg tccaggcgtg
     4441 tcctggcatt tccagcattt gctgtattac cgcaccaaga cgtggatgca gaagcaaact
     4501 gagaatgttt ttctcagtat taacctgtgc aatccactca cgcaccgcga gggtaaagaa
     4561 tgtagcgcag atctggctac acagtgcatc cactcccggc atcgccgatc tggattcctg
     4621 ttgtaaaaac gggatcgcct ccctcagcca gttatattcc acgctgtggt taaccggtgc
     4681 taaaaacagc gtttccggca aagacgttaa aaaataacgc gccgaatgtt gcaaccgaag
     4741 agtgccgcag acaatacagg tcgattcatt atcgacatga ctcaggcgat gagctgaatt
     4801 ttgtggtagc aggaccacat ttcccgggcg taatgtaaaa atctctcccg tcggcatttc
     4861 cagcttcgcc gctccttgcg ttaacgcatg ccaacgaata accgataatt ccccggcacc
     4921 atgtggaagc tgccagtcac ttcctaacac gcaattctta tcgatcgttc cttgtggagc
     4981 gttaagcatc aacaaacggc taagggcatc catatcagac tcctgagtgg tttttgatta
     5041 caaacaccaa caatcttagt aaataactaa tctaatacca tcaaatggtt aatcatgatt
     5101 tttgttttgt gattctgatt aagaaaaaga aaataccaga cgataagaca aaatatcaag
     5161 cgttgtgaag aaatggtatt tgctcttttg cgtctatagt catgatgtca aatgaacgcg
     5221 tttcgacagg aaatcatcat gaataaatat caggcagtga ttattggttt tggcaaggct
     5281 ggaaaaacat tagccgtcac gctggcaaaa gcaggttggc gagtggctct catcgaacaa
     5341 tcaaatgcaa tgtatggcgg gacctgtatt aatatcggct gcatcccaac caaaacattg
     5401 gttcatgacg cacagcagca cacagatttt gtccgtgcca tacagcgtaa aaatgaagtg
     5461 gttaattttt tacgtaataa gaattttcat aatcttgcgg atatgcccaa tatcgacgtg
     5521 atcgacggcc aggcggagtt tatcaataat catagcctgc gtgttcatcg gcctgaggca
     5581 aatctggaaa ttcatggcga gaaaattttt attaataccg gtgcacaaac cgtggttccg
     5641 ccaattcctg gaattaccac cacgccagga gtatatgaca gcaccggatt acttaatcta
     5701 aaagaattgc ctgggcattt aggtattttg ggcggcggat atattggcgt tgagttcgcc
     5761 tctatgttcg ctaattttgg cagcaaggta accattttag aagcagcttc gctgtttttg
     5821 cctcgggaag atcgggatat tgctgataat atcgcgacga ttttacgcga tcagggcgtc
     5881 gatattatcc tcaatgccca tgtggagcga atcagtcacc atgaaaatca agtgcaagtg
     5941 catagcgagc acgcccaact ggcggtggat gcactgttaa tagcttccgg tcgtcaaccg
     6001 gctaccgctt cgttacatcc agaaaatgcc ggtatcgcag taaacgagcg cggggcaatt
     6061 gtcgttgaca agcaattaca taccaccgca gacaatattt gggcgatggg agatgttacc
     6121 ggcgggctgc aatttactta tatttcactg gatgattacc gcattgtgcg tgatgagttg
     6181 ctgggagaag gcagacgtag tactgatgat cggaaaaatg tgccttattc cgtattcatg
     6241 acaccgccac tgtccagggt tggtatgaca gaagaacagg ccagagagag tggtgctgat
     6301 attcaggtgg tgacattgcc tgtagctgca attccgcgtg ccagagtgat gaatgatact
     6361 cgtggggtat taaaagcgat tgttgataat aaaacccaac gtatattagg ggcatcactg
     6421 ctgtgtgttg actcccacga gatgatcaat atagtgaaaa tggtgatgga tgccgggctg
     6481 ccttatagca tattacgcga tcagatattt actcatccgt cgatgagcga atcactcaat
     6541 gatctatttt cattagtcaa ataaactcaa aatcagacgc cagaacaaat attctggcgt
     6601 ctcagagaaa agaatcttat taattccctg ttactctata acttcttaat cacttcattg
     6661 atggtatttt atatgtttaa aaaatctgtt ttatttgcaa cactattatc tggcgttatg
     6721 gcattttcca ccaatgcaga tgataaaata attctgaaac atatcagcgt ctcgtcagta
     6781 tcagcatcac cgacagttct ggaggatacc attgctgata tagccagaaa atataatgct
     6841 tcatcctgga aagtcacatc gatgcgaatt gataataatt caaccgcaac agcagtattg
     6901 tataaataag gatgttcaca atggaaaaat acctgcacct gttaagtcgg ggagataaaa
     6961 ttggcctgac attgattcgt ctgagtattg caattgtttt tatgtggatt gggttattaa
     7021 agtttgtccc ttacgaggca gacagcatta caccattcgt cgcaaacagt ccactaatgt
     7081 cgttctttta tgaacacccg gaagactata aacagtatct gactcacgaa ggcgaataca
     7141 aaccagaagc aagggcatgg caaacggcca ataataccta tggtttttcc aacggtcttg
     7201 gcgtcgtgga ggtgattatt gctctgctgg ttttggctaa tcctgtcaat cgctggttag
     7261 gtttattggg aggactgatg gcatttacca cgccgctggt aacactctca tttttaatca
     7321 ccaccccgga ggcatgggta cccgcattgg gtgacgctca tcatggtttc ccttatttat
     7381 ccggtgctgg tcgcctggta ttgaaagata ctctgatgct ggcaggtgct gtcatgataa
     7441 tggcagattc ggcgcgggaa attcttaaac aacgcagtaa tgaatccagt tcaacgttaa
     7501 aaactgaata ttgataatcc gaacactgtc tgttgttcac tttttcaggc gtcaggcccc
     7561 ctttctgatg atgaagcctg acgccatatt accggagata tgacagagtg aagatcaata
     7621 tcactcccct gttaaaagat tcttgtcgtc tcaggtaaaa tatatgatta atatcaatca
     7681 tgtattttta cagcaagaca accacgaaca aattcaggat taaaatgttt taccgactcc
     7741 ccaacataac ccaaatcaag cgaactaatt tgtgccattt ctttctctgt cagtgagaaa
     7801 tcccagatag caaaattttc ttcgatacgc tgttgatgtg tcgatttcgg aataacgacc
     7861 actccccgtt gaatattcca acgcagaatg acttgagaaa tcgatttttg atgggcatct
     7921 gcaatactct gaagcagatt attttcaaag ggtttatgtc gtccaccacc taacggagcc
     7981 catgcttcag gctgcacgtt ataatacttc atggttgcca gcgcctctgg ttgggcaaaa
     8041 tagggatgca actcgacctg gttgaccatc ggtttaactc ttaccgtttc acaaaagttc
     8101 gccagaacat gaggatagaa attggaaacc ccaattgctt ttaatttgcc ctcttcatag
     8161 gcatcttcga gtgcacgcca cgcactgaaa taatcgcgca tagcctggtg caataaatag
     8221 aggtcgaagt actctagtcc cgattttttt aatgatgctt caatacctgc tgctgccgta
     8281 tcctgattca acatatcctg cacccacaat ttagacgtta taaataactc atctcgggta
     8341 cataaacctt cagatatagc ttcacgaacg gcttcaccga cggcatcttc attaccgtat
     8401 accgctgcag tgtcgatgag tcgataacct gtgcggatag cgttcaacac tgactgtttg
     8461 cacacgtttt tatcggtaac ctgaaaaaca ccaaatccca tcatcggcat tttcagattg
     8521 ttacttaata cggaaaattc cactgtcagt ccctccatta ggcatgaaca atgagtctac
     8581 gttaaaacgt aacctcaaat tagtacgtgg attttgatat cacttatgca aaaaattcat
     8641 taataatgca ggactgaaac ctctctattt tcggggacaa cgaagcagac gctaccagtg
     8701 cttttgcctt cgcccttgct gtttttgata cacttagggc ccagggcata acgaaaatgt
     8761 gcgatatgac gaagaatcaa tggagaatgt gatgatcagg cagaagattc tacagcagct
     8821 cctggagtgg attgagtgca atcttgagca ccctatttca atcgaagata tcgcacagaa
     8881 atctggctac agcagacgca acatccagct tctgttccga aatttcatgc atgtgccttt
     8941 gggagaatac attcgcaaac gaaggctttg tcgtgccgcc attcttgtcc ggctcaccgc
     9001 gaaatctatg cttgatattg cactctcttt gcattttgat tcacagcagt cattcagccg
     9061 cgaatttaaa aagttattcg gctgctctcc ccgtgtatac cgccaccgtg attattggga
     9121 tctcgcaaat atcttccctt cttttttaat acgtcaacag caaaagaccg agtgtagatt
     9181 agtcaacttt cctgagacac ctatttttgg caattcattt aaatatgata ttgaagtgtc
     9241 gaataaatca ccggatgaag aagtcaaact gcgacgtcat catttagcca gatgtatgaa
     9301 taattttaag acggatatct atttcgtctc cacgtttgaa ccgtcaacaa aatcggtcga
     9361 tttgctcacg gttgaaactt ttgccggtac ggtatgcaaa caaaccgatt cagagatgcc
     9421 gaaagagtgg acaattaacc gaggactgta tgcctctttc cgctatgaag gtgagtggga
     9481 gcactaccct gaatgggcgc gaaacctcta tctgatggaa ttacccgcca gggggttagc
     9541 cagagtgaac ggcagtgata ttgagcggtt ttattacaat gaaaatttcg tcgaaattga
     9601 tagcaataac attgtttgcg aaatttttat ccccgttcgt ccggtttagt tttcgctata
     9661 tctaaatgaa tttataacga gactactgtc aggaaattca ataaataatg gcgttgaaga
     9721 tgtcaacgcc attattgttt attagaatgt tactttcata ttcttaaaat tatttcatcg
     9781 cctcctcgtc ctttgccgta tcggcatcca ccgcggtaat ggtcaccgtc actggcttca
     9841 tttcgtaccc gccataggtc agggcgttaa atgtgaacgt atagacacca ggcttgctgg
     9901 tactaaattt acgcgtgatt tttccgctca cgaaatggtc atttttcgac ggcaggaact
     9961 gataatcctt ttccgtcacc ccttccggcg tctgaatgtc gacccacata ttcccgccaa
    10021 cggggttgtt tgccgacatc agtgtcacct ccagcttcgc agtcgatttc ccatccgcca
    10081 ccagagattg catatcagat gatgcggtga gtaccgcgtt tttatcatcc gcaacgaatt
    10141 tagattcggc agttttcgtt gatacctgac cgttggtctg actcacttgc gccttgagtg
    10201 tgtatgtcgc cgcaacagta gtggtagcgg tgaaaatagc ctgcccttgc tcattcgttg
    10261 tcgcagtgcc attcggttcc aggtttaacg aagccgggct ggcagtcagc gtcacttcgc
    10321 tatctttcac caaattgtta ttggcatcag tcaccgtaac tttatacgtt tgtttggctt
    10381 tgccgtccgc cagcgcatag ttgcccatca cctcaatacc ggagacggtt gcggtagcct
    10441 tatcggctac aaacgttata gtctgcgtat tcgaaggctg gctattccct gccgatgccg
    10501 tgatctcata cgttccggca gtcactgaaa ccacctgcaa ctcggctttc ccctcatcgt
    10561 tggctttcac ccagacatta tcgcctgtaa gcggcttgac accccggggc agattaaagg
    10621 tcaccaggct ccctggaaca gggttaccat ttttatcctc aacgttcgcc gtcagcgtaa
    10681 aggcatcttt gccgtttgcc actttttgag cagcggtgtc tacctgcagg tttgcctgac
    10741 cggtgctggc atccgcgttg aatttcaccg tgaccgtctt ctgagcatta ttcactgagg
    10801 ccgtgatgct gtgttccccg gcaaccgttg acatcagctc aatgtcggct ttacctgccg
    10861 catcggttgt taccgtattc cccgtcttgc tggtcacacc tttcggcaga gtcagcgtca
    10921 cattttgtcc ctgcaacggg ttaccatagg tgtccacaac ggtcagggtt acctggttag
    10981 tcgattgtcc attagccagc tggttatcaa ccttcaccgt catatcacga atctcagcct
    11041 tagatgcgtc accagcaaca ttcagcacca gtggctgagc aacggcattt tggccgttca
    11101 ctcgcggcat cacagacaac tggcccgcgg cagatcccag cgttaaggtc gccacgtaga
    11161 ccccattact tgtttctgtc cagtctcctg ttgaaggtcg ctctgtcccc gtgctggcgg
    11221 caccactaaa ctgcggtgca tctggcttca gcccggtgac aaggttcccg tacgcatcct
    11281 tcacggtgaa ggtgagtttc gtcgtcgttt tgacggtcgg agccttattg tcagcaacaa
    11341 gcgtagagtt cgctgatgac atctcccccg caatgacagt caactgcgcg gcttcggtgg
    11401 ctgctggctg gccgttgaat agcggcatga cgagaagctc acccgtcttt ccacctgttg
    11461 tcagcgtagc gacataggaa ccatcacctt tttcggtcca accagaaaca gtcgcccctt
    11521 cagacgcagc acccgtcagg cttgccgaca gggaaagacc actgatagcg ttgccatgtg
    11581 catcttttgc aataagcgtc acggttgtgc tttcgcctgc ttttacgcga tctgcggcta
    11641 cagacactgt cgactggcct gaagataacg catcagccac cacggttact ttagccgcat
    11701 tcgctgccgc atcctgtcca tttagcttcg gcataacctc taattcaccc gccgtagtgc
    11761 cgagagaaat ctgcgcagtc caggtcccgt cgccattatc cgtccagcct gatgccgtag
    11821 aacctacagc agctgcgccc gataatgacg gtgcatccgg attgagacca gttacagggt
    11881 tgtcattagc atcttttgcc gtccagattg ccgtaactgt accgccaacc accggtttat
    11941 caggattcag tgtgatggac gaatgtgctg catcaagcgg cccggcaaca aacttcagcg
    12001 tttgttgtaa tgtgcccagc tgatactgct cgctgaaagc acgcacaaca acgtttccgg
    12061 cacgggttga agaaaccgtg gcgctgtaaa cgcctggttt tatttccgaa atggcaccaa
    12121 cggttacacc attagtgtct tgcggaacaa atcgcaagcg gctggcttct cccgtcaccg
    12181 gattaccctc ggagtccacc gcagtcaacg tcaacgtata ggcttgctga ccatcagcaa
    12241 ccacatcacc tgacggctcg ttagcgctca gggtggagtt tgccacatcc atcatcgtgg
    12301 cccgcagttc tgcagtgacg gttttgctca tgccatcaac gctaacagta atcgttgcct
    12361 cacctgactg cgttccggta gtaaagacag actgatacac ccctgcttca gtttcggtga
    12421 actcacccag tgttggcttt gcctgtgatt tagtggcctt tagggaacga gtcacaatat
    12481 ttccagccgg tttgaaggct agttcagtct tgatctgatc tttcatgccc gtgactggct
    12541 gcccctcggc gtcgcgcaga gacagcacca gcggtctttg ctcattaccg ttagcaagca
    12601 tttgaatacg gctctgaccg tcaagcgtta acgccgtgcg atcggcgctc atacctgctc
    12661 cggtaatgac cacctctgtc tgcacgcgtt ttgaggcatt gcctttgtta tcgtaggcaa
    12721 ccgcagaaat cgcataataa ttgtctttgc ctggacgata agccgggagc gttacttgcc
    12781 actgactacc ctgaccggta attttgccac cctcagccag taatgacggc gcttcccact
    12841 gcacattttt cagtccgtga gttgctttgc tgaccacaag ccccagggaa agtgtctgac
    12901 cacccttacc ttcaatacgt tcaggcagag caatacggat cacttcagat ttgcggtact
    12961 caagaacgat gttgttatta cgctcaacca ggtcatagcg gctgcctgcc agtacccgac
    13021 gctcgcgaat gctgtctgta tcgagttgtt tcgccagagg ttcgccaatc cgataattaa
    13081 cttccaggcc aaagcgagtg tcattctcac cactcttgcc ctgcttatgc ccggcgctca
    13141 gtgtcagaag aggcactggc gtataggtca cctcggcaga aatagcatgc gggtctttct
    13201 ggcgcttatc tttaccaaac agcccgactt catcgccata atactgttca tacatcaggc
    13261 ttgcgccaag ctgcggccag gcgggtaaat agccctcagc acgaatatcc cagccattcg
    13321 ccgggcgttc ctgataatcc tcaacatccg gcgatttttt ccagccagaa gcccggatat
    13381 aaccattggc gctcagtttc agataatcgc gccagtattc cgcaccaaca ccaatgcggg
    13441 tatgactacg ggataaatca tggtcgataa aggtgttcac ccccgccatc cagtcatttc
    13501 ctgaaaaatg acgccagcca aaaccaatat ttgactgagt acgatcgtcg gtacgatgta
    13561 ttgctccctg agtgaacaac atatttgttg gcgtatcata aatcggataa agcatttcca
    13621 gcgaagaatc cttcagcgag aaatctttat cgacattcag tttgacgcgc gcagtaccat
    13681 atttcccgag ccactcctgt atttcctggt tagctttagc tgtggccatt ccggtaataa
    13741 agttacgtgt cgcatcgcta tctggctgac tgcttaaaaa tgtcccggca tttgcggcaa
    13801 acgacgcgac atttttctcc acgttattat cagcagttac cgtagtattt cccatgctca
    13861 accgtggctg aaccgcatgc tgcgcacgtg ccgccattac tggggtaaag gtgacagcga
    13921 gtggaaaaag aacctgaaca gagatatttg cccacgccac gcagcgggtc agaactgaat
    13981 aacgaaatcg tggttgttta tgacctgttt tataatgtga cattgagata atttcctgat
    14041 gaacgaatta acgaacatag gcagacacaa ataatggcgt ctgcttgtca gaaaaaccgg
    14101 ggatttcccg atttcctttt ccgcagaaaa ggaaatacct ttatgtaaga aagtgcaccg
    14161 ccacctaata tatccagtgc ctgtagccac tca
//
LOCUS       DAZGOB010000133        13869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGOB010000133 DAZGOB010000000
VERSION     DAZGOB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.133"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..963
                     /locus_tag="ACHLIF_004090"
     CDS             <1..963
                     /locus_tag="ACHLIF_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO9607996.1"
                     /translation="KQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSND
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEGKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            1232..3469
                     /gene="nrfB"
                     /locus_tag="ACHLIF_004091"
     CDS             1232..3469
                     /gene="nrfB"
                     /locus_tag="ACHLIF_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO9607997.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMFNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3456..6428
                     /gene="nfrA"
                     /locus_tag="ACHLIF_004092"
     CDS             3456..6428
                     /gene="nfrA"
                     /locus_tag="ACHLIF_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO9607998.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6429..7319
                     /gene="ybcH"
                     /locus_tag="ACHLIF_004093"
     CDS             6429..7319
                     /gene="ybcH"
                     /locus_tag="ACHLIF_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO9607999.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7502..8263
                     /gene="envY"
                     /locus_tag="ACHLIF_004094"
     CDS             7502..8263
                     /gene="envY"
                     /locus_tag="ACHLIF_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO9608000.1"
                     /translation="MQLSSSEPCVVILTEKEVELSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8776..9729
                     /gene="ompT"
                     /locus_tag="ACHLIF_004095"
     CDS             8776..9729
                     /gene="ompT"
                     /locus_tag="ACHLIF_004095"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO9608001.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9916..11400
                     /locus_tag="ACHLIF_004096"
     CDS             9916..11400
                     /locus_tag="ACHLIF_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9608002.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(11584..11796)
                     /locus_tag="ACHLIF_004097"
                     /pseudo
     CDS             complement(11584..11796)
                     /locus_tag="ACHLIF_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            11946..12614
                     /locus_tag="ACHLIF_004098"
     CDS             11946..12614
                     /locus_tag="ACHLIF_004098"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9608003.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(12559..12696)
                     /locus_tag="ACHLIF_004099"
     CDS             complement(12559..12696)
                     /locus_tag="ACHLIF_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO9608004.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(12669..13253)
                     /locus_tag="ACHLIF_004100"
     CDS             complement(12669..13253)
                     /locus_tag="ACHLIF_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9608005.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEEIKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(13253..>13869)
                     /locus_tag="ACHLIF_004101"
     CDS             complement(13253..>13869)
                     /locus_tag="ACHLIF_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO9608006.1"
                     /translation="FDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHSHVDGRRFN
                     TSTFKDTYQYGSTAVGSNSYQVQGAVGLGIGTMANTNSAGAHTHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT         3772 a         3376 c         3273 g         3448 t
ORIGIN      
        1 aagcaatatg gtgattttga aaatggtatt cctgttcacg atactattgc cagagttgta
       61 tcctgtatca gtcctgcaaa atttcacgag tgctttatta actggatgcg tgactgccat
      121 tcatcaaatg ataaagacgt cattgcaatt gatggaaaaa cgctccggca ctcttatgac
      181 aagagtcgcc gcaggggagc gattcatgtc attagtgcgt tctcaacaat gcacagtctg
      241 gtcatcggac agatcaagac ggatgagaaa tctaatgaga ttacagctat cccagaactt
      301 cttaacatgc tggatattaa aggaaaaatc atcacaactg atgcgatggg ttgccagaaa
      361 gatattgcag agaagataca aaaacaggga ggtgattatt tattcgcggt aaaaggaaac
      421 caggggcggc taaataaagc ctttgaggga aaatttccgc tgaaagaatt aaataatcca
      481 gagcatgaca gttacgcaat cagtgaaaag agtcacggca gagaagaaat ccgtcttcat
      541 attgtttgcg atgtccctga tgaacttatt gatttcacgt ttgaatggaa aggtctgaag
      601 aaattatgcg tggcagtctc ctttcggtca ataatagcag aacaaaagaa agagcccgaa
      661 atgacggtca gatattatat cagttctgct gatttaaccg cagagaagtt cgccacagca
      721 atccgaaacc actggcacgt ggagaataag ctgcactggc gtctggacgt ggtaatgaat
      781 gaagacgact gcaaaataag aagaggaaac gccgcagaat tattttcagg gatacggcac
      841 atcgctatta atattttaac gaatgataag gtattcaagg cagggttaag acgtaagatg
      901 cgaaaagcag ccatggacag aaactatctg gcgtcagtcc ttgcggggag cgggctttcg
      961 taatcttgcc ctgcctgctc acctcctcca ggcacttcca ctacattatg acctgctcgg
     1021 tgccaccgct ccttttcact atgacgttaa cctcccctcc ttgacaccat gcttttgtct
     1081 acattctacg caaacggcaa cttttataac cagtgtaaaa ataacgtgcc gcaatagtca
     1141 tacctattaa tggtaaaaag ctgtcacaat tcataaaaaa ccttaatata cgccacccta
     1201 aacataacca gcgttaatgt aaggtttttg tgtggactgg cttcttgatg tttttgcaac
     1261 ctggctctac ggcttaaaag taatcgcgat aacgttagcg gtcatcatgt tcatcagcgg
     1321 gctggacgat ttttttattg atgtcgtcta ctgggtacgc cgcattaaac gcaagttgag
     1381 tgtttatcgc cgctacccgc gaatgagtta ccgcgaactg tataaaccag atgaaaaacc
     1441 gttagcgatt atggttccgg cgtggaatga aacgggcgtc atcggcaata tggccgagct
     1501 ggcggcgacc acgctcgact acgaaaacta tcatatcttt gttggcacct accccaacga
     1561 ccccgatact caacgtgatg ttgacgaagt gtgcgctcgc tttccgaacg tgcataaggt
     1621 agtctgcgcg cgtcctggtc ccaccagcaa agccgactgt ctgaacaacg tgctggacgc
     1681 catcacccag tttgaacgta gcgccaattt cgcttttgct ggttttattc tgcatgacgc
     1741 cgaagatgtg atttcaccga tggaattgcg tctgttcaac tatctggtcg agcgtaaaga
     1801 tctgattcag atcccggtgt atccgttcga acgcgaatgg acgcacttca ccagcatgac
     1861 ttacattgat gagttttcag agctgcatgg caaagatgtt ccggtgcgtg aagccctcgc
     1921 cggacaagtg cccagcgcag gcgtcggcac ctgtttcagc cgccgcgccg tgaccgctct
     1981 gttagctgac ggtgacggta ttgctttcga cgtgcagagt ctgactgaag attacgacat
     2041 tggtttccgc ctgaaagaga aaggtatgac ggaaattttt gtccgttttc cggtggtgga
     2101 cgaagccaaa gaacgcgagc agcgtaaatt tttacagcac gcacggacgt caaacatgat
     2161 ctgcgtgcgc gaatatttcc ccgatacctt ttcgactgca gttcgacaaa aatctcgctg
     2221 gatcatcggc attgttttcc agggctttaa aacccacaaa tggacctcca gcctgacgct
     2281 gaactacttt ctctggcgcg accgcaaagg ggcaatcagt aactttgtca gcttcctcgc
     2341 gatgctggtg atgctccagc ttttgctgtt gctggcgtat gaaagtttgt ggcccgatgc
     2401 ctggcatttc ctttctattt ttagcggcag cgcatggtta atgaccctgc tgtggctaaa
     2461 ctttggcttg atgtttaacc gcatcgtgca gcgggtgatt ttcgtcactg gctactacgg
     2521 tctgacgcag gggctgctat ctgtcctgcg tcttttctgg ggcaacctga ttaacttcat
     2581 ggccaactgg cgcgcgctaa aacaggtact tcaacacggc gatccacgtc gcgtcgcgtg
     2641 ggataaaaca acgcatgact tccccagcgt gactggcgat acccgctcgt tgcgcccgtt
     2701 aggccaaatt ctgctggaaa atcaggtcat cactgaagaa caactcgata cagcactgcg
     2761 taatcgcgtc gaaggtctac gcctgggcgg ttcaatgctg atgcaggggc tgattagcgc
     2821 cgagcagctg gcacaggcgc tggcagagca aaacggcgtg gcgtgggaat ccatcgatgc
     2881 ctggcagatc ccgtcctcgc tgattgccga aatgccagcc tccgtagcgc tgcattatgc
     2941 agtactgccg ctgcgtctgg aaaatgatga gttaattgtc ggcagtgaag atggtattga
     3001 ccctgtttcg ctggcggccc tgacgcgtaa agtcggacgc aaagtgcgtt acgtcattgt
     3061 tctgcgggga caaattgtca cggggttacg tcactggtat gcacgccgac gcggtcacga
     3121 tccgcgggca atgttgtaca atgcggttca gcatcagtgg ctcacggaac agcagaccgg
     3181 tgaaatctgg cggcaatatg tgccgcatca gttcctgttc gccgaaatac tgaccacgct
     3241 cggtcatatt aatcgttcag caattaacgt gttgttattg cgccatgaac gcagttctct
     3301 gccgctcggc aagtttttgg tcaccgaagg cgttatcagc caggaaacgt tggatcgcgt
     3361 cctgacaatt caacgcgaat tacaagtttc gatgcaatca ctattactca aagcaggttt
     3421 aaacacagaa caggttgcgc aactggagtc cgaaaatgaa ggagaataac cttaatcgcg
     3481 tcatcggatg gtctggttta ctgctgacgt ctttattgag taccagcgca ctcgcagaca
     3541 atatcggcac cagcgcagaa gagctggggc tgagcgatta tcgccatttt gttatttatc
     3601 cccgtctcga taaggcgctg aaggcacaga aaaataacga cgaagcaacc gccatccgcg
     3661 aatttgaata tatacaccag caggtgccgg ataatattcc gctgacttta taccttgcgg
     3721 aagcctatcg ccattttggt catgatgacc gggcgcggct gttgctggag gatcaactga
     3781 aacgtcaccc aggagatgcc cgacttgagc gcagtctggc ggctattcca gttgaagtga
     3841 aaagcgttac gactgttgaa gaactgcttg cccagcaaaa agcgtgcgat gctgcgccga
     3901 ccctgcgttg tcgcagtgaa gtcgggcaga atgccctgcg gctggcacag ttacctgtcg
     3961 ccagagcgca actgaacgat gcgacgtttg ctgcatcgcc ggaaggaaaa acgctgcgaa
     4021 ccgatctgct gcaacgggca atctacctga aacaatggtc ccaggcagat acgctataca
     4081 atgaagcacg ccagcagaac acattaagcg cggcagaacg ccgtcagtgg tttgacgtgc
     4141 ttcttgccgg gcagctggac gatcggatcc tggcactgca atcacagggg atcttcaccg
     4201 atcctcagtc atatattact tacgcgaccg cgctggctta tcgtggcgaa aaagcacgcc
     4261 tccagcatta tctcattgaa aataagccgc tgtttaccac ggacgcacaa gagaaaagtt
     4321 ggctctatct gttatctaaa tacagcgcca accccgttca ggcattggcg aattatacgg
     4381 tgcagtttgc cgataaccgc cagtatgttg ttggcgcgac gctaccggtg ctgttaaaag
     4441 aaggtcagta cgacgcagcg caaaaactgc tcgccaccct ccccgccaat gaaatgcttg
     4501 aggagcgtta tgctgtcagc gtggcgaccc gtaacaaggc tgaatctctg cgtctggcac
     4561 gattgctgta tcagcaagaa ccggcaaatc ttacccgcct agatcaactg acctggcaac
     4621 tgatgcagaa cgagcagtca cgcgaagctg ccgatttatt gctgcaacgc tatcctttcc
     4681 agggcgatgc gcgtgtcagc cagactttaa tggcgcgact ggcgtctctg ctggaaagtc
     4741 atccttacct ggcaactcca gcgaaggtgg cgattttatc aaaacccttg ccgctggcgg
     4801 cgcaacgtca gtggcaaagc cagttgccgg gtattgcaga taattgcccg gcaatagttc
     4861 gcttgctggg cgatatgtcg ccttcctacg atgccgccgc ctggaaccgt ctggcaaagt
     4921 gttatcggga cacgctaccc ggtgtggcct tgtatgcatg gcttcaggcc gaacaacgac
     4981 aaccgaacgc ctggcaacat cgtgcggtag cctatcaggc gtatcaggtt gaggactacg
     5041 ccaccgcact ggcggcctgg cagaaaatca gtcttcacga catgagcaat gaggatctgc
     5101 ttgctgctgc caataccgcc caggcggcag gaaatggtgc agctcgcgat cgctggctac
     5161 aacaggcaga acaacgtgga ctgggaaaca atgccctcta ctggtggctg catgcgcaac
     5221 gttacattcc tggtcagccg gaactcgcac tgagcgatct cacgcgctca atcaatattg
     5281 cgccttctgc caacgcttac gttgcgcggg cgacaattta tcgccaacgt cataatgtcc
     5341 cggcggcggt gagtgatttg cgcgccgcgc tggaactgga accgaataat agcaacatcc
     5401 aggcagcgct tggttacgcc ttgtgggata gcggtgatat cgcacagtcg cgggaaatgc
     5461 tcgaacaggc gcataaaggg cttccggacg atccggcact gatccgacaa ctggcctacg
     5521 tgaaccagcg tctggatgac atgcctgcga cgcagcacta cgcccggctg gtgattgatg
     5581 acattgataa tcaggcgctg ataaccccac tgaccccaga gcaaaatcag caacgcttca
     5641 atttccgccg tttgcatgag gaggtcggtc gccgctggac gttcagtttc gattcttcta
     5701 tcggcttgcg ttccggggca atgagcaccg ctaacaataa tgtcggcggc gcagcgccag
     5761 ggaaaagcta tcgtagctac gggcaactgg aagccgagta ccgcatcgga cgcaatatgc
     5821 tgctggaagg cgacctgctc tcagtttata gccgcgtctt tgccgatacc ggagaaaacg
     5881 gggtgatgat gccggtgaaa aatccgatgt ccggcaccgg tctgcgctgg aagccgctgc
     5941 gcgatcagat ctttttcctc gccgtcgaac agcagttgcc gctgaacggc caaaatggcg
     6001 catccgatac catgctgcgc gccagcgcct cattctttaa tggcggcaaa tacagcgacg
     6061 aatggcaccc gaacggttca ggctggtttg cccaaaacct gtacctcgat gcggcgcaat
     6121 atatccgcca ggatattcag gcgtggacgg cagattatcg cgtcagctgg catcagaagg
     6181 tagctaacgg acagactatt gagccttacg ctcacgttca ggacaacggc tatcgtgata
     6241 aaggcactca gggcgcgcag cttggcggag tcggggtccg ctggaatatc tggaccggcg
     6301 agacgcacta cgacgcctgg ccgcacaaag tcagtctcgg cgtcgagtat caacatacct
     6361 ttaaggcgat taatcaacgt aacggagagc gcaacaacgc gtttctcacc attggagtgc
     6421 actggtaaat gcgtaagttc attttcgtat tgctgacact gcttttggtc agcccttttt
     6481 cctttgcgat gaaaggtatt atctggcaac cacaaaaccg agatagtcag gttaccgata
     6541 cccagtggca ggggctgatg agtcagttac gtttgcaagg cttcgatacc ctggttttgc
     6601 aatggacccg ttacggcgat gcattcaccc agccagaaca gcgcgcgtta ttgtttaagc
     6661 gtgccgcagc tgcgcaacag gctggcctga agctgattgt cgggctgaac gccgatccgg
     6721 aattttttat gcaccagaaa cagtcgtccg cagcgctgga aagctatctt aatcgcctgc
     6781 tggctgccga tctccagcaa gccagattat ggagcgccgc gcctggcgta acgccggatg
     6841 gctggtacat cagcgcggaa attgacgacc tgaactggcg cagcgaagct gcccgtcagc
     6901 ctttgctaac atggttaaac aacgcgcagc ggctgattag cgatgtttca gcaaaaccgg
     6961 tttatatcag tagttttttc gccggaaaca tgtcgcccga tggctatcgc caactgctgg
     7021 aacaagttaa agcaaccggc gttaatgtct gggtacagga tggcagcggc gtggataaac
     7081 ttaccgctga acagcgtgaa cgttatctac aggccagcgc cgattgccaa agtcccgccc
     7141 ctgccagcgg cattgtttat gaactgtttg tcgccggcaa aggcaaaacc tttacagcga
     7201 aaccgaaacc ggatgcagag atcgcctcgc tgttagcgaa acgttcctct tgcggtaaag
     7261 acactctcta tttctctctg cgctatttgc ccgtcgcgca cggcattctc gagtattaaa
     7321 tctcctccag gtaagtcggg tacgacctgg cttacctctt tcgctctcgt cagaattcca
     7381 tcaagattaa ttgctaaaaa gcgtgctaaa gaaagaagat cgttatcggc gaacgggccg
     7441 atcactcatg caggaagcat agatcctttg agcggagttt ctgatatctg aggagtgcga
     7501 aatgcaattg agcagcagtg aaccttgcgt ggtgatcctg accgaaaaag aggtagagct
     7561 aagcgtcaat aaccatgcta cgtttaccct tccgaaaaac tacctggccg ccttcgcgtg
     7621 caacaataac gtcattgaac tctcaacgtt aaatcacgta ttaatccccc acatcaaccg
     7681 taacatcatc aacgattatc tgttgttttt aaataagaac ttaacctgtg taaagccctg
     7741 gtcgcggctg gcaaccccgg ttatcgcttg tcatagccgt acaccggaag tgttccggct
     7801 agccgccaac cacagcaagc agcaacccag caaaccctgc gaagcggagt tgacgcgcgc
     7861 attacttttt accgtattgt ctaactttct tgagcaatcg cggtttatag ccctactgat
     7921 gtatatctta cgcagcagcg tccgcgacag cgtctgccgc attattcaaa gcgatattca
     7981 gcattactgg aatctgcgaa ttgtcgccag ttcgctatgt ttaagcccca gcctactcaa
     8041 aaagaaatta aaaaacgaaa ataccagcta tagccagatt gtcacagagt gtcgtatgcg
     8101 ttacgccgta cagatgttat tgatggataa caaaaatatc actcaggtgg cgcaattatg
     8161 tggctatagc agcacgtcgt actttatctc tgtttttaag gcgttttacg gcctgacacc
     8221 gttgaattat ctcgccaaac agcgacaaaa agtgatgtgg tgaagggcaa agcggaaacg
     8281 gataagacgg gcataaatga agaagaaatg gcgcgccctg caggattcga acctgcggcc
     8341 cacgacttag aagttcctag aacgacattt taagtcaaca aattaccgcg ccatctctgc
     8401 gctcacacgt cccactacct caaaacatgt aaagccttgc aagccattgt gaggccttat
     8461 gtgtctcagt tttgtccctc ttttttgtac taaaaaacat agcaattgag gataaacctc
     8521 atgctatttt cgcttatatg cctttaaagg catggcgctt aaatagataa aaacaccaca
     8581 aaagcataaa aaaccacaca gtaaaaccgc aacataaaac aataacagat aattaaacca
     8641 aaaacagata gcgcattgtg ataatcattc aatactaaac aaaatataaa cagtggagca
     8701 atatgtgatt gactcattaa gttagatata aaaaatacat attcaatcat taaaacgatt
     8761 gaatggagaa cttttatgcg ggcgaaactt ctgggaatag ttctgacaac ccctattgcg
     8821 atcagctctt ttgcttctac cgagactata tcgtttactc ctgacaacat aaatgcggac
     8881 attagtcttg gaactctgag cggaaaaaca aaagagcgcg tttatctagc tgaagaagga
     8941 ggccgaaaag tcagtcaact cgactggaaa ttcaataacg ctgcaattat taaaggtgca
     9001 attaattggg atttgatgcc ccagatatct attggggctg cgggctggac tactctcggc
     9061 agccgaggtg gcaatatggt cgatcaggac tggatggatt ccagtaaccc cggaacctgg
     9121 acagatgaaa gtaggcaccc tgatacacaa ctcaattatg ccaacgaatt tgatctgaat
     9181 atcaaaggct ggctcctcaa cgaacccaat taccgcctgg gactcatggc cggatatcag
     9241 gaaagccgtt atagctttac agccagaggt ggttcctata tctacagttc tgaggaggga
     9301 ttcagagatg atatcggctc cttcccgaat ggagaaagag caatcggcta caaacaacgt
     9361 tttaaaatgc cctacattgg cttgactgga agttatcgtt atgaagattt tgagctaggt
     9421 ggcacattta aatacagcgg ctgggtggaa gcatctgata acgatgagca ctatgaccca
     9481 ggaaaaagaa tcacttatcg cagtaaagtc aaagaccaaa attactattc tgttgcagtc
     9541 aatgcaggtt attacgtaac acctaacgca aaagtttatg ttgaaggcgc atggaatcgg
     9601 gttacgaata aaaaaggtaa tacttcactt tatgatcaca atgataacac ttcagactac
     9661 agcaaaaatg gtgcaggcat agaaaactat aacttcatca ctactgctgg tcttaagtac
     9721 accttttaac gaagttaacc agattttctc cccggtggta ttgtataacc ccggggaatt
     9781 tgtttacagt tacaagtagg caatatccac taattttgta agcatttaac ctatgtcata
     9841 acgtgtaatc tcttaccgca acacaagacc tcaatatatt caaatcttat ctttattccg
     9901 atattcagag acacaatgcg aaaaataatt actcatttca aagttgtttt aacgttactt
     9961 ctaccagtaa ccgtatctgc ccagcagata cagtggcaat cctgtatggc cagtcaattc
    10021 aaccactggt ttggtgagga aaaaccgtct cctgacttac tatgtggtta tttgtctgtt
    10081 ccattaaaat atacagacac aggcggagat gcttcttatg aaaaaaaatc acaagtcaaa
    10141 ctagcgttga caaaattgcc ggcaaaaagc aagcataaag gaagtatcct gataataagt
    10201 ggtggtcccg ggttaccagg cataaatcct tatattaact ttgactggcc agtcacaaat
    10261 cttcgtgagt catgggatat tattggattt gatcctcgag gcgtcggaca gtccactccg
    10321 acaataaact gccggcaatc agatacagag actcaggaaa acataaccga aaagcaacaa
    10381 gtattaaata aaattaatgc ctgtatccat aataccggag ccgaagtcat tcgccatatc
    10441 ggctctaacg aggctgtata cgatattgat cgtattaggc aagccttggg ggataaacaa
    10501 ctgacagccg tggcgtattc gtatggaact caaattgcag ccttatatgc agaacgtttt
    10561 ccctacaacg taagatctat cgttcttgat ggagtcgtcg atatcgatga cctggaggac
    10621 aacttcacat ggcaactcaa acaggcacag agttatcagg aaacgtttga tcgctttgca
    10681 tcctggtgtg cgcgtacaaa aagttgcccg ctttcttcag acagagataa ggcaataact
    10741 cagttccatg agctattatc aaaattacat cacaaacctt tattagacag taagagagaa
    10801 aatatatctt cagatgaact catatcatta acaacagacc ttctgctatg gcgttcatca
    10861 tggccaaccc ttgcaactgc catacgccag ttctctcagg ggattgtcag taatgaaatt
    10921 gaaactgcgc tcagtgctcc gatagcctca gaagagtcaa gcgatgcttc gggggtaatc
    10981 ctctgtgtag atcaggggga tgagcaatta acaccagaag agcgaaaatc ccgaaaagac
    11041 gctcttgcga atgccttccc ggctattaac tttgacaatg gacgttccga ttcacctgat
    11101 ttttgtgaat tatggccaat acatagcgac ctgaacaaaa ctcgcctgaa aaatactgtt
    11161 ctgccctctg gtttactgtt tatagcacac aaatacgacc caacaacgcc ctggattaat
    11221 gcccgtaaga tggcagagaa attttccagc ccgttactaa caataaatgg tgatgggcat
    11281 acattagctc tcaccggagt taatttatgt gtagataaag cagttgtaca tcacctgatc
    11341 actccacaaa aaatagaaaa tatatactgc ccaggaaatt ctgaagcaga aatacaataa
    11401 tttcaaaata ttccgctatt tgctcccaat gcaaaacata tattgcattg ggagcatatt
    11461 catatttttg ttattattgt atgaaatcgt ctcatgtaca cggtcctttt ttcgccgaaa
    11521 actgatatta ctttaatcaa aagttgacgt gccagattcg aagtaaattt ataaaatatc
    11581 aaatcaatat acattttgca ccttcgagat agtaatgact gttactgtaa catgagttac
    11641 aaacagcttc atacccagag ctactgatgt atcaactcga ttcaacaata cccgatattt
    11701 attccatgcc tccagcaacg atctttcttc ctccgttgcg atttccagat ctacagcatc
    11761 ctgcagtggc gcaatatact cactgaattc ctggatgtag aactgtgtgg tgacggtctt
    11821 ccagccattc ggctcctgtt gtatcgaagc ataccaggct atttcaatat cgctatgctg
    11881 cggcagcatt taaccccttg taattcatcg ccataattga tttaattcac aaataaaact
    11941 ataacatggt gaaattaatg aaaaaaaaca cagatgatgg ggctaaaatt tacacaccac
    12001 ttaccctaaa gctttatgac tggtgggttt tgggagtatc aaatcggctt gcatggggat
    12061 gtcctacaaa ggaacacctt cttccacact ttctggaaca tttaggtaac aaccatctgg
    12121 atattggtgt tggaactggg ttttacctta ctcacgtacc tgagagtagt ctgatatctt
    12181 taatggattt gaacgaagct agcctgaacg cggcatctac aagggctggg gaatcaaaaa
    12241 ttaaacataa aattagccat gatgtttttg aaccttatcc cgcggcgtta catggtcaat
    12301 ttgattccat ttccatgttt taccttcttc actgcctgcc tggaaatata tctacaaaaa
    12361 gctgtgtaat acgcaatgcg gcgcaggcct taactgacga tggaactcta tacggagcca
    12421 caattcttgg cgatggagtt gtgcacaata gcttcggtca aaaactgatg cgcatttaca
    12481 atcagaaagg catcttttca aacacaaaag attccgaaga aggcttaaca catatactct
    12541 cagagcattt cgagaatgtt aaaaccaagg ttcaaggtac tgtagtaatg ttttccgctt
    12601 cagggaaaaa atagcatcca accgcagcac gttcttgctt aagacgtgct gcggcataat
    12661 cccaatgatt actccctgac agggttcgta ggccactcaa tatcaggtgc agttgatgta
    12721 tcaacacggt tcagcaacac ccgatacttt ttccaggctt ccagcaatga ggtttcttcc
    12781 tccgttgcga tttccagatc tacagcatcc tgaagtggcg caatatgctc actggctacc
    12841 tgcatcaggc tgttttttat ttcttccgcc tctcggatcc ggaacagttt ttctgcttct
    12901 gcatctttca cccaggctgt gccgttccac ttctgaaact ccccttccgg cgataaccag
    12961 gtaacatttt ccggtaacgg accgagttca gaaataaata acgcgtcccc tgacgctacg
    13021 tcataaaccg ttttaccccg atggtcttca acgagatgcc acgatgcctc atcactgttg
    13081 aaaacagcca caaagccagc aggaatatct ggtggtgcaa tatcggtact gtttgctggc
    13141 agacctgtat gaggcggaat atatgcatca ccttcaccaa taaattcatt agttccggcc
    13201 agcagattat aaatttttat ggtccgtgct tgttcactca ttctgaatgc cattatgcaa
    13261 gcctcacaat atagttaaat gcgatgtttt tgacggtgtt ttccgcgtta ccagcagcgt
    13321 taacggtgat ggtgtgtcca tgtgagccaa tcgcaacgga gtgcgtatga gcaccaatac
    13381 cgacagtatg tgcgtgtgca cctgcgcttg cagcagtgcc ggacagtgag tgggtatgcg
    13441 cgccagcaga gttcgtattc gccatcgtgc caatgcccag accaaccgcc ccttgtacct
    13501 ggtatgagtt acttccgaca gctgtagaac catactgata ggtgtcttta aaagtgcttg
    13561 tattaaacct gcgaccatca acgtgcgagt gtgctccggc cgagcttgta gagccgctca
    13621 gactgtgcgt atgagccccg gtgttattcg tggatttagt gccgtaatca aacgacgatg
    13681 tggttttcgt ccccaaatcc gtactggatg cgctggcgct gtgggtgtgc gatttaatgc
    13741 cgtcctgttc ctgagacaat acggcacgac cactggcggg tttgccctta atcgtccagc
    13801 cacgcatatc agggatcacg cctgacggat aagcggctgc aagtttcggg tatgcagatt
    13861 tgtcaaaag
//
LOCUS       DAZGOB010000134        13496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGOB010000134 DAZGOB010000000
VERSION     DAZGOB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.134"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..880
                     /gene="yneK"
                     /locus_tag="ACHLIF_004102"
                     /pseudo
     CDS             1..880
                     /gene="yneK"
                     /locus_tag="ACHLIF_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1030..2220
                     /gene="ydeA"
                     /locus_tag="ACHLIF_004103"
     CDS             1030..2220
                     /gene="ydeA"
                     /locus_tag="ACHLIF_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO9608007.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2245..2910)
                     /gene="marC"
                     /locus_tag="ACHLIF_004104"
     CDS             complement(2245..2910)
                     /gene="marC"
                     /locus_tag="ACHLIF_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO9608008.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     MYH"
     gene            3122..3556
                     /gene="marR"
                     /locus_tag="ACHLIF_004105"
     CDS             3122..3556
                     /gene="marR"
                     /locus_tag="ACHLIF_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO9608009.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3576..3959
                     /gene="marA"
                     /locus_tag="ACHLIF_004106"
     CDS             3576..3959
                     /gene="marA"
                     /locus_tag="ACHLIF_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO9608010.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3991..4209
                     /gene="marB"
                     /locus_tag="ACHLIF_004107"
     CDS             3991..4209
                     /gene="marB"
                     /locus_tag="ACHLIF_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO9608011.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4266..5705)
                     /locus_tag="ACHLIF_004108"
     CDS             complement(4266..5705)
                     /locus_tag="ACHLIF_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO9608012.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(5730..7403)
                     /locus_tag="ACHLIF_004109"
     CDS             complement(5730..7403)
                     /locus_tag="ACHLIF_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO9608013.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(7459..7770)
                     /locus_tag="ACHLIF_004110"
     CDS             complement(7459..7770)
                     /locus_tag="ACHLIF_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO9608014.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(7798..9120)
                     /locus_tag="ACHLIF_004111"
     CDS             complement(7798..9120)
                     /locus_tag="ACHLIF_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO9608015.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFDLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(9235..9546)
                     /locus_tag="ACHLIF_004112"
     CDS             complement(9235..9546)
                     /locus_tag="ACHLIF_004112"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO9608016.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            9745..10443
                     /locus_tag="ACHLIF_004113"
     CDS             9745..10443
                     /locus_tag="ACHLIF_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9608017.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGRYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(10488..11387)
                     /gene="eamA"
                     /locus_tag="ACHLIF_004114"
     CDS             complement(10488..11387)
                     /gene="eamA"
                     /locus_tag="ACHLIF_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO9608018.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            11582..12769
                     /gene="ydeE"
                     /locus_tag="ACHLIF_004115"
     CDS             11582..12769
                     /gene="ydeE"
                     /locus_tag="ACHLIF_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO9608019.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            12896..12991
                     /gene="mgtS"
                     /locus_tag="ACHLIF_004116"
     CDS             12896..12991
                     /gene="mgtS"
                     /locus_tag="ACHLIF_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO9608020.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12991..13095
                     /gene="mgtT"
                     /locus_tag="ACHLIF_004117"
     CDS             12991..13095
                     /gene="mgtT"
                     /locus_tag="ACHLIF_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO9608021.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(13210..>13496)
                     /gene="dgcZ"
                     /locus_tag="ACHLIF_004118"
     CDS             complement(13210..>13496)
                     /gene="dgcZ"
                     /locus_tag="ACHLIF_004118"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO9608022.1"
                     /translation="YRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITHSEGHIN
                     ITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT         3685 a         3206 c         2867 g         3738 t
ORIGIN      
        1 cgctatacaa tgaaaaggtt cagtttaaac tcacttatcc cgtcaagaat ggaagacaca
       61 aagaaaatat tgaatttcag gtagtaataa atttaagccc tatttattta gataattttc
      121 gccatgatgg agaaattaat atttttgcgc tcccaaccca aagcctgtca ctatggggcg
      181 cgtatttcag accggcgtcg agcgtgtgct atttctgttt ctgaatgatt ttattgaaca
      241 atttccaatg atcaaccttg gcgttcccat caaaagagcg catacaccac atattgaacc
      301 cctgcctccg gatcgccata ccgctgcaga ttacttacgc cagtttgatt tgcttgtcct
      361 gaattttatc tctcgcggta attttgtcat actcccccga ttatggagta actctgaggt
      421 tcacagatgg ttcgtcaata aagatcctaa cttgatcacc gccattctcg acataacaga
      481 cggcgaatta aaagaggatt tattgcaaag cctgatggat tcattaggtt ctaacaaaca
      541 tgtactaccc gaagtctgca tctgcttttt atccctttta gcagaacaag aatctcctca
      601 ttttcaggac ttttttttat ttttcgccaa tatgttattg cactatcacc aatttatgaa
      661 tcccaatgaa agtgatctga atgacgtgtt gatgccagca tcattaagtg atgataaaat
      721 tatcaaacat atggcgcgca ggaccctcaa actgtttgta aaaaatgaaa cgccgccaaa
      781 agttactcac gaagacctgg tgaaaaacag gcctcgctcc cctgtcagac cacctatacc
      841 cgcaaccgcc aaaacgccag acctccctga acgtcattaa accgtgatgt taccgactct
      901 ctgacgcgga acagaatcag cgtcagagaa acggaaaacg cgatccagat cacaaatgca
      961 ttgtattcac atcattaacc gttttaagat catttcatca ctttttcgca actcacccga
     1021 taatctgtta tgacaacaaa cactgtttcc cgcaaagtgg cgtggctacg ggtcgttacg
     1081 ctggcagtcg ccgccttcat cttcaacacc accgaatttg tccctgttgg cctgctctct
     1141 gacattgcgc aaagttttca catgcaaacc gctcaggtcg gcattatgtt gaccatttat
     1201 gcatgggtag tggcgctaat gtcattgcct tttatgttaa tgaccagcca ggttgaacgg
     1261 cgcaaattac tgatctgcct gtttgtggtg tttattgcca gccacgtact gtcgttttta
     1321 tcgtggagct ttaccgttct ggtgatcagt cgcattggtg tggcttttgc acatgcgatt
     1381 ttctggtcga ttacggcgtc tctggcgatc cgtatggctc cggccgggaa gcgagcacag
     1441 gcattgagtt taattgccac cggtacagca ctggcgatgg tcttaggttt acctctcggg
     1501 cgcattgtgg gccagtattt cggttggcga atgaccttct tcgcgattgg tattggggcg
     1561 cttatcaccc ttttgtgcct gattaagtta cttcccttac tgcccagtga gcattccggt
     1621 tcactgaaaa gcctcccgct attgttccgc cgcccggcat tgatgagcat ttatttgtta
     1681 actgtggtgg ttgtcaccgc ccattacacg gcatacagct atatcgagcc ttttgtacaa
     1741 aacattgcgg gattcagcgc caactttgcc acggcattac tgttattact cggtggtgcg
     1801 ggcattattg gcagcgtgat tttcggtaaa ctgggtaatc agtatgcgtc tgcgttggtg
     1861 agtacggcga ttgcgctgtt gctggtgtgc ctggcattgc tgttacctgc ggcgaacagt
     1921 gaaatacacc tcggggtgct gagtattttc tgggggatcg cgatgatgat catcgggctt
     1981 ggtatgcagg ttaaagtgct ggcgctggca ccagatgcta ccgacgtcgc gatggcgcta
     2041 ttctccggga tatttaatat tggaatcggg gcgggtgcgt tggtaggtaa tcaggtgagt
     2101 ttgcactggt caatgtcgat gattggttat gtgggcgcgg tgcctgcttt tgccgcgtta
     2161 atttggtcaa tcattatatt tcgccgctgg ccagtgacac tcgaagaaca gacgcaatag
     2221 ttgaaaggcc cattcgggcc ttttttaatg gtacatttta atgatttcca ggatgccgtt
     2281 aataataaac tgcacaccca tacataccag caggaatccc atcagacggg agatcgcttc
     2341 aatgccaccc ttgcccacca gccgcataat tgcgccggag ctgcgtaggc ttccccacaa
     2401 aataaccgcc accaggaaaa agatcagcgg cggcgcaacc atcagtaccc aatcagcgaa
     2461 ggttgaactc tgacgcactg tggacgccga gctaataatc atcgctatgg ttcccggacc
     2521 agcggtactt ggcattgcta gcggcacaaa ggcaatattg gcactgggtt catcttccag
     2581 ctcttccgac ttgcttttcg cctccggtga atcaatcgct ttctgttgcg gaaagagcat
     2641 ccgaaaaccg ataaacgcga cgattaagcc gcctgcaatt cgcagaccgg gaatcgaaat
     2701 gccaaatgta tccatcacca gttgcccggc gtaatacgcc accatcatga tggcaaatac
     2761 gtacaccgag gccatcaacg actgacgatt acgttcggca ctgttcatgt tgcctgccag
     2821 gccaagaaat aacgcgacag ttgttaatgg gttagctaac ggcagtaaca ccaccagccc
     2881 caggccaatt gctttaaaca aatctaacat tggtggttgt tatcctgtgt atctgggtta
     2941 tcagcgaaaa gtataagggg taaacaagga taaagtgtca ctctttagct aaccttgcat
     3001 cgcattgacc aaaacttgaa ccgatttagc aaaacgtggc atcggtcaat tcatttattt
     3061 gacttatact tgcctgggca atattatccc ctgcaactaa ttacttgcca gggcaactaa
     3121 tgtgaaaagt accagcgatc tgttcaatga aattattcca ttgggtcgct taatccatat
     3181 ggttaatcag aagaaagatc gtctgcttaa cgagtatctg tctccgctgg atattaccgc
     3241 ggcacagttt aaggtgctct gctctatccg ctgcgcggcg tgtattactc cggttgaact
     3301 gaaaaaagtg ttgtctgtcg accttggagc actgacccgt atgctggatc gcctggtctg
     3361 taaaggctgg gttgaaaggt tgccgaaccc gaatgataag cgcggcgtac tggtaaaact
     3421 taccaccagc ggcgcggcaa tatgcgaaca atgccatcaa ttagttggcc aggacctgca
     3481 tcaagaatta acaaaaaacc tgacggcgga cgaagtggca acacttgagc atttgcttaa
     3541 gaaagtcctg ccgtaaacaa aaaagaggta tgacgatgtc cagacgcaat actgacgcta
     3601 ttaccattca tagcattttg gactggatcg aggacaacct ggaatcgcca ctgtcactgg
     3661 agaaagtgtc agagcgttca ggttactcta aatggcacct gcaacggatg tttaaaaaag
     3721 aaaccggtca ttcattaggc caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa
     3781 agctgaagga aagtaacgag ccgatactct atctggcaga acgatatggc ttcgagtcgc
     3841 aacaaactct gacccgaacc ttcaaaaatt actttgatgt tccgccgcat aaataccgga
     3901 tgaccaatat gcagggcgaa tcgcgctttt tacatccatt aaatcattac aacagctagt
     3961 tgataacgtg acaacgtcac tgaggcaatc atgaaaccac tttcatccgc aatagcagct
     4021 gcgcttattc tcttttccgc gcagggcgtt gcggaacaaa ccacgcagcc agttgttact
     4081 tcttgtgcca atgtcgtggt tgttccccca tcgcaggaac aaccaccgtt tgatttaaat
     4141 cacatgggta ctggcagtga taagtcggat gcgctcggcg tgccctatta taatcaacac
     4201 gctatgtagt tattgtcagg ctctgatcac agagcctgac atctccgtaa tatcttcctc
     4261 aagtctcaaa tattttcgcc attgttggcg atgagatccc gataccagta aaacgacgct
     4321 ttacgtatcc gttccagcgt tcccttccct tcgttgtctt tatcgacata aatcattccg
     4381 tagcgctttt tcatttcccc tgttccggca gaaaccaggt caatgcagcc ccacggggtg
     4441 tagccaatta agtcaacacc atcttcaact acggcttttt tcatttcccg aatatgggaa
     4501 gcaaagtaat caatgcgata gtgatcgttc accgtgccgt cagcttgtct ctggtcaacc
     4561 gcaccaaatc cattttcgac aataaacagc ggcaactgga aatgatccca gaaccagttg
     4621 agtgaataac gtagccctgc cggatcaatt tgccatcccc attcggatgt atcgatataa
     4681 gggttgctga ccaaatctcg tggttcaaca taatccagtt gcgggttatc gggcgaaaat
     4741 tgtgtcgtaa aagacatgta gtagctaaag ccgataaagt cgacacaacc actggcaaga
     4801 atcgcgttat cttctggtgt gatatcgagg ttgaatcctt tcctggcaaa gtaattcagc
     4861 atatgttgcg gataatatcc acgagcatga acatcagtaa accagtaacg acgatgcatt
     4921 gctttcgtgg ccatcatcat atcgttgggt gcacacgtca gaggatagat gggacacatg
     4981 gcgatcatac agccaatgtt aaattcagga ttgatcgatt ttccagtctg taccgccagg
     5041 gcactggcaa ctaactcgta atgtaccgcc tggtacatta tttgttcgcg ctcattgata
     5101 tcttcctctg gcgaatacaa gataccggaa ttagtaaatg gacacaggct ttcgctgaaa
     5161 ttcacctgat tattgatttc gttaaacgtc atccagtact ttactttttc tttatagcgc
     5221 gtgaagaccg ttgatgcgaa gcggataaaa aagtcgatca gtttacggtt tcgccagcca
     5281 ccatattttg tcaccagatg ataaggcatc tcaaaatgtg aaagcgtcac cacaggttcc
     5341 attccctgct taaggcattc atcgaacaga tcatcataaa attgtaaacc ctcttcattc
     5401 ggctcctgtt cgtcaccttg cggaaagatt cgtgtccagg caatggaagt tcgaaagcat
     5461 ttgaatccca tctcggcaaa taactgaata tctgttttat agcgatgata aaaatcaatt
     5521 gcttcatgat tgggataatt aagcccgtcg ataacgcctt ctgtcacttc acgcggcacc
     5581 ccgtgagcgc cagcagtcat cacatcagcg atgctgatgc cttttcctcc ttcattccag
     5641 ccaccttcca actgatgcgc ggctaccgca ccgccccata aaaaaccttt tttaaatcct
     5701 gacatacaca actccttaac taaaatagat taccaccaga cttccgcctg gatgccggcc
     5761 ataaactcgt ctttgctgtt ctcattaaac ttaaaattcg ataattcgtt atccagaata
     5821 ttgatatacg ttccatagaa gcgaatttct ggtcgtgaac ctaaaatgct ttcacccact
     5881 ttcaatgcat gccagagtgt cgttttataa gccgattcat ttagtgttac accctgctga
     5941 gttttgttct gttgcttaaa ccagcctaat tcaagccccg tctgattcca tgtattccag
     6001 atccaggccg gtcttattac agtgcgaata ctgtcaaaat cactatgagc accactttca
     6061 taactataaa catcttcgcc gtgagaatag acaagcgcgt tagccataat aatattgtca
     6121 gtaagataca tctcgccctg agagattaaa cgccaggcga tcccattggt atgatcacca
     6181 tagtaatagc gaccatgcgc catcgtatta ctggcatctg agaaactggc aaaactgctg
     6241 gcataggaat tattggcaac ctgtaacgta aattcattaa acgtatcgcg ttgcaggttt
     6301 tgccgtaaaa cagaagtcag catccagcta tctttcattt caaaataact gttgtcattt
     6361 tcgttatctt gttgttgatc cgttttatta ggtgcggaat atttagccat taatgataat
     6421 gtcgcatcat cccataacgg gatattgcga taacgaacat ccacagaatt ggtattcatc
     6481 tggctggtac gcgaaaaatc acgagagtaa acatcgacat catcacggct taaggacata
     6541 tcaaacagcc ctacaccaag tgcccagttt tcaatcccca cacccgcggc gacatccgtg
     6601 gttaaggttt tccagtccag catctggatc tcatattgcg ggagtttatg tttgccgacc
     6661 cagaagtctg cctctggtgc gaagggtaaa aaacctcgcg ttgtcaggta aatatcgctg
     6721 aactgcatga tattttcatt ggcactgtca ccaaaccagg catcgttata ctgctcaccc
     6781 acgttgccat cataggtaac gaccgcattt gccgttttac cgtcctgatt ataaacacgc
     6841 tgatttaagg tcaggtcaaa ccaaccgctc atctcattac caaaacgtcc cagagaaccc
     6901 gctgcataag tttgtggtga gccgtgatta ccagtacccc agcctgagcg gaagtacccc
     6961 tgatagctga acccaatatc atcctttata tatttactga tatctttcag ggtcacgctt
     7021 tcgatagtgg ttttgtcgcc tttactttca ataacggcaa cttgctgcga tccatttata
     7081 gtatgcgctg cggtaaacga gttctgttca ccgttttcat ttttgatgtt atcagcgacg
     7141 ggagatgtcg ctgatatttc agcaagcgag gctgatttat atttgttttc cgtgatgctt
     7201 ttttgtaagg tcgaaagtcg ggatttatat actcccagtt cattctgtgt tgctttcagc
     7261 tcttgtttat tttgcgacaa ttcattttca agcaattcaa ggcgctgctc tatggttaat
     7321 tttgttgcca tcgcgttcaa tggaaacaat gctgtaataa tagaaaaaga caaaaggctg
     7381 acgttaagcg tctttatatt catgacttat ccttaagagt ataataattt cctccagcta
     7441 tcgcactgga ggaaaaaatc aggcatggag ttcttttctt aatgaaataa tttccctggc
     7501 taaatcctgg cataatattg cattcatcaa atgatcctgt gcatgaatca gaattaaatt
     7561 aacttttatt ttgccacagc cttcatcctg actaattaat gcggtctgaa tcttatgtgc
     7621 ctgaagacag gcagattcag aactggccat tagctgagtt gcgccctgcc agtctttttg
     7681 cccggcacaa cgtatggctt ccatcgcgtc agaacgtgcc tgccccgcgt tgaccagtaa
     7741 ctccataaca agttcttcat ctgcaaacat cacaatctcc ttcctgggta aatggaatta
     7801 tccgttagtt gtcatgttat tgactttgtt ggcggcaata acaaaaggta aatagatcat
     7861 tgtcgccaca cctaaacata aaaaaccaac cagtaaagcc agccagttcc ccccggttcc
     7921 gaggaaagca attaaaggtc cgggcgtagt ccagggcacg gcataagcaa ccggtggaat
     7981 aatttccatc gaaacaaaga agtaaccaat ggcacagtta acaatgggaa ccataataaa
     8041 cggcaccatc atgatggggt taagtacaat aggaaggtca aacattatcg gttcattgat
     8101 attgaaaata ccggggataa atgacatttt tgccagatca cggtattccg cacgcttaga
     8161 agcgataaaa atagccaaca ataaccccaa cgtcatacct gaaccgccgc agttggcgaa
     8221 tgcatcatta atagaagtcc aggtaatcgg atatggcgcg ccccaggtag tgccatgttg
     8281 agcggcccag gagagattct ccagattagc ctcagaaaaa acggtttctc gaattgccga
     8341 tgtggtatta ggtccgtgga tccccagcac ccacaggaaa ttacccacag cccccaggat
     8401 aattaccgca aagatattcg ttcccatatg tttgagcggc gtctggatta atgtgtaaat
     8461 aagatcgttt agtcctgccg gggatatcag tgtcaggcaa taattaagtg cggaaaagaa
     8521 caccatgaca aaaaatattg gcaataaaac tttaaatgaa cgcgctaccg caggcggtac
     8581 agctgccggc atcgttatgg tgatttttgg gtttcgagct aagcgacaaa atatttcact
     8641 ggtgatcaat gcaacgataa cagcaacaaa caacccctgc gcgccgagat atttggtcgt
     8701 caggaatgct tgcccatcta tcatctgata tggcgtataa acaataaaaa atgctcctat
     8761 tgccgttaat ccgcataaaa gatcatcttg ctcatagcta atcgccatat tcctggcgac
     8821 caggaaagca atcataattg acatgatatt tacagaacca ttcattaccg gagtaaaaat
     8881 agcttgtgct ttttcaaggt tggggaaaat gctattcaga tgcagtaatc cggcaataaa
     8941 accgtctggc gataatatgg caaagttaat taatataatt aatgaacttg ccatgataat
     9001 tggaaacgta aaaacgaatc catctctcac cgctgcaaca tgtttttgtg agtttaactt
     9061 gatagcaact ggaacaagaa aacgttccat tccacgttcg aatgaggcca ttaatcccat
     9121 ttttttatac ctgttgtttt ggatatttat acatcccaat aaagcatgta tgccatatcc
     9181 attttatgat gtgtgaaaag ttagttcagg taatagttta ataatattag attcttactc
     9241 ttgcagtagt ttcatcgcaa attcaagaac tgcctgtccg ttcatggtgc cgtagtcttt
     9301 catctcgata acggctacag gcattttccc ctgtgcaacg gcttcaatct ctggtttctg
     9361 aaagcgaact tgcgggccaa gtaaaacaac gtcaacttcg ttatttttaa ttttcccttt
     9421 agcctctgca atcgccaatg cggaaatatt aacttcaagt gaaatagcgt tagcatgatc
     9481 aatcatacgt ttaaccagca tactggttga catgcccgca gcgcaaacta acaatatttt
     9541 tttcatattt taccttactg gtatataaca gattacattg tttaaatatc atatcccgca
     9601 gtagctatac gtcctggtac attgatcaca gtaatacagt gataaagttg ttcaacatca
     9661 gggtgattat taaacgactc ggccatacga taaaccaatc aggactcaac tccttgtttt
     9721 caatggctct taaaaggtgc aaaagtgatt tttcaaaaga ttgcccgctt acttaaatcc
     9781 gaaatcaatg gcaattcatg gcatgttggt gatttgttgc cgtcagaagc ggaactcgct
     9841 gttcgttata atgtttcgcg taataccctg cgtaaggcat tatccttgct ggaaggcgaa
     9901 ggaattattc acagaaagca tggttcagga agatacattc aaaaaaagaa ttttgttgca
     9961 cacattgatc acatgaacag tttcagtgaa attgcgcata aaagcggcaa agaggcaggg
    10021 agtcagatta tgaaatttga agtgcaggat gcttcaccca ctattgcgac tgagctgaat
    10081 ttagtgactg gtgagcaggt ttattacata aagagactgc gattcattga ggataatgca
    10141 gcacaattag aagaaacgtg gatgtcagta gcacgttttc ctgatttaac cgtgtcacat
    10201 atgcaaaaat ccaaattttc atatattgag aatgaatgcg ggatcaaaat cattggcacc
    10261 tttgaaactt tctcccctac ttttcctacc ccagaaatcg ccagtatttt acggatcagc
    10321 ccacgggatc ccatacttaa aattcagacc caggctgtgg acagtaactc tattccgctg
    10381 gattattcgt tactttacag caatattttc gagttccagg taaagtactt tttcccgcga
    10441 taaaagcaga cgcacagagt gctgacatca cattctgtgc gtatggttta gcctctaacc
    10501 tttaccgctt tacgccaccg caagccaaat acattgatat acagcccggt cataatgagc
    10561 accgcaccta aaaattgcag acccgttaag cgttcatcca acaatagtgc cgcacttgcc
    10621 agtccgacta cgggcaccag taacgataac ggtgcaaccc gccaggtttc atagcgtccc
    10681 agtaacgtcc cccagatccc ataaccaaca attgtcgcca caaacgccag atacatcagc
    10741 gacaagatgg tggtcatatc aatagtaacc agactgtgaa tcatggatgc ggaaccatcg
    10801 agaatcagcg aggcaacaaa gaagggaatg attgggatta aagcgctcca gattaccagc
    10861 gacatcaccg ccggacgcgt tgagtgcgac atgatctttt tattgaagat gttgccacac
    10921 gcccaactaa atgctgcagc cagggtcaac ataaagccga gcatcgccac atgctgaccg
    10981 ttcagactat cttcgattaa caccagtacg ccaaaaatcg ctaaggcgat ccccgccaat
    11041 tgtttgccat gcagtcgctc cccgaaagta aacgcgccaa gcacgatagt aaaaaacgcc
    11101 tgtgcctgta acaccagcga agccagtcca gcaggcatac caaagttaat ggcgcagaac
    11161 agaaaggcaa actgcgcaaa actgatggtt aatccatacc ccaagagcaa attcagtggt
    11221 actttcggtc gtgcaacaaa aaagatggcc ggaaaagcga ccagcataaa gcgcaaaccg
    11281 gccagcatca gcggtggcat gttatgaagc cccactttga tgaccacaaa atttagcccc
    11341 catacgacca ctaccagtag cgccaacacc ccatcttttc gcgacattct accgcctctg
    11401 aatttcatct tttgtaagca atcaacttag ctgaatttac ttttctttaa cagttgattc
    11461 gttagtcgcc ggttacgacg gcattaatgc gcaaataagt cgctatactt cggatttttg
    11521 ccatgctatt tctttacatc tctaaaacaa aacataacga aacgcactgc cggacagaca
    11581 aatgaactta tccctacgac gctctaccag cgcccttctt gcctcgtcgt tgttattaac
    11641 catcggacgc ggcgctacgc tgccatttat gaccatttac ttgagtcgcc agtacagcct
    11701 gagtgtcgat ctaatcggtt atgcgatgac aattgcgctc actattggcg tcgtttttag
    11761 cctcggtttt ggtatcctgg cggataagtt cgacaagaaa cgctatatgt tactggcaat
    11821 taccgccttc gccagcggtt ttattgccat tcctttagtg aataacgtga cgctggtggt
    11881 gctctttttt gcccttatta actgcgccta ttccgttttt gctaccgtgt tgaaagcctg
    11941 gtttgccgac aatctttcgt ccaccagcaa aacgaaaatc ttctcaatca actacaccat
    12001 gctaaacatt ggctggacca tcggtccgcc gctcggcacg ctgttggtga tgcagagcat
    12061 caatctgccc ttttggctgg cagctatctg ttccgcgttt cccatgcttt tcattcaaat
    12121 ttgggtaaag cgcagcgaga aaatcatcgc cacggaaaca ggcagtgtct ggtcgccgaa
    12181 agttttatta caagataaag cactgttgtg gtttacctgc tctggttttc tggcttcctt
    12241 tgttagcggc gcatttgctt catgcatttc acaatatgtg atggtgattg ctgatggaga
    12301 ttttgccgaa aaggtggtcg cggttgttct tccggttaat gctgccatgg tagttacgtt
    12361 gcaatattcc gtgggccgcc gccttaaccc ggttaacatc cgcgcgctga tgacagcagg
    12421 caccctctgt ttcgtcatcg gtctggttgg ttttattttt tccggcaaca gcctgctatt
    12481 gtggggtatg tcagctgcgg tatttactgt cggtgaaatc atttatgcgc cgggcgagta
    12541 tatgttgatt gaccatattg cgccgccagg aatgaaagcc agctattttt ctgcccagtc
    12601 tttaggctgg cttggtgctg caattaaccc attagtgagt ggcgtagtgc taaccagcct
    12661 gccgccttcc tcgctgttta tcatcttagc gttggtgatc attgctgcgt gggtgctgat
    12721 gttaaaaggg attcgtgcaa aaccgtgggg gcagcccgcg ctttgttgat ttaagtcgaa
    12781 cacaataaaa atttaattca gccttcgttt aggttacctc tgctaatatc tttctcattg
    12841 agatgaaaat taaggtaagc gaggaaacac accacaccat aaacggaggc aaataatgct
    12901 gggtaatatg aatgttttta tggccgtact gggaataatt ttattttctg gttttctggc
    12961 cgcgtatttc agccacaaat gggatgacta atgaacggag ataatccctc acctaaccgg
    13021 ccccttgtta cagttgtgta caaggggcct gatttttatg acggcgaaaa aaaaccgcca
    13081 gtaaaccggc ggtgaatgct tgcatggata gatttgtgtt ttgcttttac gctaacaggc
    13141 attttcctgc actgataacg gatcgttgac acagtagcat cagttttctc aatgaatgtt
    13201 aaacggagct taaactcggt taatcacatt ttgttcgtca ataaacatgc agcgatttct
    13261 tccggtttgc ttaccctcat acattgcccg gtctgctctt ccaatgacca catccagagg
    13321 ctcttcagga aatgcgcgac tcacacctgc tgtcacggta atgttgatat gcccttcaga
    13381 atgtgtgatg gcatggttat cgactaactg gcaaattctg acacctgcac gacatgcttc
    13441 ttcatcatta gccgctttga caataatgat aaattcttcg cccccgtagc gataaa
//
LOCUS       DAZGOB010000135        13493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGOB010000135 DAZGOB010000000
VERSION     DAZGOB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.135"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            121..1179
                     /locus_tag="ACHLIF_004119"
     CDS             121..1179
                     /locus_tag="ACHLIF_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO9608023.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1189..2331
                     /locus_tag="ACHLIF_004120"
     CDS             1189..2331
                     /locus_tag="ACHLIF_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO9608024.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2324..3097
                     /locus_tag="ACHLIF_004121"
     CDS             2324..3097
                     /locus_tag="ACHLIF_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608025.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3099..4178
                     /locus_tag="ACHLIF_004122"
     CDS             3099..4178
                     /locus_tag="ACHLIF_004122"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO9608026.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4178..5134
                     /locus_tag="ACHLIF_004123"
     CDS             4178..5134
                     /locus_tag="ACHLIF_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO9608027.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5145..6353
                     /locus_tag="ACHLIF_004124"
     CDS             5145..6353
                     /locus_tag="ACHLIF_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO9608028.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6371..6838
                     /gene="terW"
                     /locus_tag="ACHLIF_004125"
     CDS             6371..6838
                     /gene="terW"
                     /locus_tag="ACHLIF_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO9608029.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7099..7428
                     /locus_tag="ACHLIF_004126"
     CDS             7099..7428
                     /locus_tag="ACHLIF_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9608030.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7415..7795
                     /gene="tnpB"
                     /locus_tag="ACHLIF_004127"
     CDS             7415..7795
                     /gene="tnpB"
                     /locus_tag="ACHLIF_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9608031.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            7838..8668
                     /locus_tag="ACHLIF_004128"
                     /pseudo
     CDS             7838..8668
                     /locus_tag="ACHLIF_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8699..10312)
                     /locus_tag="ACHLIF_004129"
     CDS             complement(8699..10312)
                     /locus_tag="ACHLIF_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO9608032.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10343..10693)
                     /gene="tnpB"
                     /locus_tag="ACHLIF_004130"
     CDS             complement(10343..10693)
                     /gene="tnpB"
                     /locus_tag="ACHLIF_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9608033.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10690..11115)
                     /locus_tag="ACHLIF_004131"
     CDS             complement(10690..11115)
                     /locus_tag="ACHLIF_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9608034.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11169..11920
                     /locus_tag="ACHLIF_004132"
                     /pseudo
     CDS             11169..11920
                     /locus_tag="ACHLIF_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            13303..>13493
                     /locus_tag="ACHLIF_004133"
     CDS             13303..>13493
                     /locus_tag="ACHLIF_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608035.1"
                     /translation="MEFSVIFFWVITGLITILFLKSIITDLIKNLNKTDIALYILAFL
                     SAGYTFQYIYNDNIFSELWG"
BASE COUNT         3272 a         3527 c         3495 g         3199 t
ORIGIN      
        1 actgacgcac tccataacca caggagttat taatcatatt atgattgaca tgtagcttag
       61 acactcatag actcacttct caatcataaa tttgcatggc gttccggata attaaaaaaa
      121 atgaacaaga aaatttggtt tatggaaggt ttatcctccc agcgagatat tattcagggg
      181 gtaaaatcat ttgcacaaaa aaataatttt gccattaccg tttttgcctc ccaccgtaac
      241 gaaagaaatg aaatcctttc cgttgccgat tattctttga ctgaacctga agatcctcaa
      301 aaacgtcttc agtttatcca ggaaaccatt cagacctacg gcatccacca tattcatact
      361 ggccgtaaca gccagtggtt tgaagaacac cgttcagcca ttgaaccgac cggtgccacc
      421 cttactaccg gtgcaacggg cgtcgactgg ttaactctgg ctgacgaaaa agttactttt
      481 gctcagttta tggagcaaaa gggtctcccg gtcgtaccat cctggcgggt gaatacgctg
      541 gcagaattaa agacacacct cgcggccccg ccgttcactg acagcccggt atgcgtgaag
      601 ccggtgacgg gtatctatgg catgggattc tggcgctttg atgacagtgc ttcgcctatg
      661 gccgtcttta atcatcccga acatcgtctg gtcagtccgc aacagtatat tgcagcagca
      721 tcagctgctg agtcgtttaa accccttgtt ttgatgccgt acctgccagg cccggaattt
      781 tccgtcgata tcctcgcgga taagggcgaa atactcgcag ccgtgggacg ccgtaaggaa
      841 ggggctatcc agtatctggt aaacgaagga agcgcctggg aactggcgtg tgactgcgcc
      901 cgtgttatga aggccgacgg gctggtgaat gttcagacgc gaaacgatgt gaatggcaac
      961 ccggtgctgc ttgaaaccaa catgcgtccg tcaggggggg tgggttatac ccttcacagc
     1021 ggtgtgaacc ttcctgggtt atttgctgcc tttaagctcg gtctgatgtc tgaagatatg
     1081 gtacgccaga gcgctaaaaa caccttttct ccggttgcgg tgagatccat tacggatgta
     1141 attgcatacc cggaatcact ctctaacctt ctgaattaag gcgtatttat gtctgatatc
     1201 ctcgaagata tgatttatcg ccgtaccctc tcatgcggta cgattcaggt aacccgtgac
     1261 cagggtgaag tttcgctcga tgacctgttt gacatcgctg aaagacgtaa cccgaaacgc
     1321 gcctttctgt ttgtcagcaa agtactcggc aggcacattc ccgtgccacc ctcagtcatg
     1381 cgacaggcct acagacagct tgccagccag ttcccctcga cactgacagg acccgtactg
     1441 tttatcggta tggctgaaac cgccgttggg cttggggccg gtgtatttga tgaagtgcgc
     1501 caccagcatc gtgaatctgt ctatctgacc tctacccgtc acccggttga tggcacgttg
     1561 ctttgcgagt tcaaggaaga acacagccac gccaccgatc atctgatcta tctgccagat
     1621 gatgaagaga aaagacgtcg tgttaccaac gcacgaacgc tggttttgat tgatgacgag
     1681 gcaaccaccg gtaatacctt tattaacctg ctttctgccc tgcgtaatac cggcaagctt
     1741 caacatattg aacaggttat cgccgtaacg ctgaccgact ggagtggcaa tgccctgtcg
     1801 gagcgcaccc ccttgcccgt cacttcggtt tcgcttgtaa gcggtcagtg gggatggacc
     1861 ccattacctg atgcacctgt cccggacatg ccgaaagtca acgtaacttc acgaggagaa
     1921 tgggacatcc aggggaaaca gtcatggggc cgactgggga tgctcgcacc tgcggccgat
     1981 ctcggccatg aggtctccgt ccacaagggg gaacgtattc tggttctcgg gaccggggaa
     2041 ttcgtctggg agccgttcct gcttgctgaa cggctcgaag ctgccggagc acaggcattt
     2101 tatggatcga ccacccgctc ccctgtcgcc gttggttatg ccattgagtc cgccatttcc
     2161 tttacggata actacgggct gggcatcccc aattttgtct ataacgtcgc ccaccagcag
     2221 tttgaccgca ttcttgtgtg tactgagaca cccgcagaaa gtattgacac gcagcttctt
     2281 aaagcgctgg ctgaggttgc gcccgtcgtg gagattgtta cctatgaata aacccgttgt
     2341 tctgagcgac cttgacgaca cgctctttca gacccgtcgt aaaatggtgg acgagctggc
     2401 cctggagcca ttccgtaccg gtgccgttga tcgcaccctg aatccacgaa gctttatgac
     2461 ggaagaacag tccatgttgg tggactggct cctggagcag gctgaactca tacccgttac
     2521 cgcacgggga actgaagaaa tcagccgcgt acggatccct ttccactcct gggcaatcac
     2581 aacgcacggg gccgtcattc tcacgcctga aggcaaaccc gacgaggagt ggaaggccca
     2641 tatgctcggc cagctggctc cctaccagga aaagctgaca tcgatgcagc gtctgatcac
     2701 tgaaatgatg gacgcaaaag gaatcaatgc ctgggcaagg ctgaacttcg aatacggtga
     2761 aacggcggtt tatatggtga tgaaacaccg cgacagcacc cgccttgacg agctcaatgc
     2821 cattgcagat gagatagaaa cggtgtttcc gaccgagggc ttctacatcc accgcaacag
     2881 taataacgtg gcctggcttc ccaccccggt tgagaaaggg ctggcagtca gctggctgct
     2941 tgaaaaactt cgggctgaac gaggagtttt ccccgtaatt ggtttgggcg acagcctgag
     3001 cgatcatcgt tttatgaaac tgtgcagctg gtttggcatc ccgcgtcaga gtcagtttgc
     3061 agatgccatt tcacagcgaa tttttggaga aaattaaaat gcagccacat gaaacattta
     3121 ccggctcata ccagcccggt gacgtggaat ttctgctaaa gccggttgtc attgagatga
     3181 cgccggttga gaaaaaagaa gaactgattc agtccgggaa gaaacattat tcggacatgc
     3241 tcagccagga gccagcgcct acacaatggc atcttgattt gtttcaccgg gcgctggatc
     3301 ggggcgcaga gaggcttgca aaagaagtca cacagctcgc tattgctctg gccgaacgct
     3361 tcggtgatga gcccattgta ctggccagtc ttgtcagggc tggcgttccg ctcggcgtta
     3421 tgctgcacca ggccctgcgt gacatgggga aaacctcatg gcattacggt atcagcatta
     3481 tccgggatcg tggaattgac ggtgcagccc tcgacgtcat tgaagagcgg catggtacca
     3541 gcggtattgt ctttgttgac ggatggacag gtaaaggcgc aattaccgga gagcttgtac
     3601 gcgcactgaa agatcgcccg ggctatcctg agcagccgcg actggttgtc ctggccgatc
     3661 cctgtggctg ctcctggctt gcagccagtg atgatgactg gctcattcct tttggcatca
     3721 tgggggcgcc ggtatcaggc ctgatctccc ggtcagtatg gtcctctgaa ggattacacg
     3781 ggtgcatggt ttgcgagcat ctcagtgaat tcgaatgcag ccggatgctt gccgataccg
     3841 tcgctcattt ccgtaagaag ttaacaccgt catccctcgc tcccctgagc tggaatacgg
     3901 aatcagcccg ggtcttatgg cagacaagtc gcgacgttat cgcgttcctg gccgatgaat
     3961 ttaaagtgga cagcgtcaat cgtattaaac ccggtattgc tgaagcaacc cgggctgtat
     4021 tacgtcgggt accggaccat gtatttgtgc gttctattga cgacccggac gttgccttgc
     4081 ttgtagggct tgctcgtgaa aagggaatag ttgttacaga aatgggggga accctcggcc
     4141 agtatcgggc tgtaaccatt atcaagaagg tactctgatg aaaaatcgtc tttctccctg
     4201 gaatctggga gccacgctat acatgcctgc aacacgggaa gatattgctg atgccgtcct
     4261 gcacgggaaa atcccgggct tgcgctctct ggtgatttgc ctggaggatg ctgtcagtga
     4321 agcagacatc cccgtcgccc tcaaaaatct ggagcacctg ctgcacgagc tcagtaacag
     4381 catgagtagc ctgggtaaaa atgactggcc tctggtgttc attcgccccc ggcatgccga
     4441 aatgggcaga tggttaaaag cgcattatga tctttccgct gtcgacgggt ttgtgctgcc
     4501 taaatttacc ctcagttcgt tagctgaatg gtgggatatc atggtcggga ctcacctgtg
     4561 catgatgcca acgctggaaa cagaagatgt ctttgacgtg gttcagatgc gcgagctggc
     4621 tacccgtctg gaggaacatc cctgccacga ccgcattatt gcgcttcgaa tcggcggcaa
     4681 cgatcttatg aacgttgtgt cgcttcgccg tccccgggac ctgacgctgt atgacagccc
     4741 gatgggctac gtcatcaaaa tgctcgtttc ggtcttcggc ccgcgtgatt ttgccctgac
     4801 cgcgcctgta tgtgagcata ttgacgatca tgccgttatg gccagagagc tggctcttga
     4861 tatggcacac gggcttgttg ggaaaacggc cattcacccg ggtcagatag aggtcattca
     4921 aaacgcgctg atggtcactc agggtgagca ttctgacgct ctgcggatcc tgaactctac
     4981 ccaggccgtg tttaagtcgc agggagcaat gtgtgaaccc gccacacatc gccgctgggc
     5041 ggctggcatt ctggacaggg ctcgttttta tgggttacag aacgagcaaa gcgctgatgg
     5101 aatcagatta cttaccgtga cccagcatca ttaaggaaat cataatgggg ttcaggttca
     5161 gaaagtcgat caacattatt cctggcgttc gcctcaacct gagtaacggt gcaccgagcc
     5221 tgagtgtcgg gccgagaggt gcttccgttt cttttggtag ccgggggacc tatgccaatc
     5281 tgggcttgcc cggtaccggg ctgagttacc gtacccggct tgaccgggcc gcgcgttcca
     5341 gaggtgaaaa ccggacggca accgacccgg ggctcagaca ggcgcttgag cagaaagccg
     5401 ctgaactcat gtcagcggta accgcaatcc gtaatattca cgagctgacg ccggatccaa
     5461 aaacaggcat cagctgggca gagctggaag cagtatacct gcataacaga acgtcgcctt
     5521 ttcaggttcc ggcaccggtg cgtccagaaa agccagacta ccttgtattg ccggaaaagc
     5581 ctgccgagag cgaaggcatt agttttttgg gtaaatggtt tgaatcggaa tcagctaaag
     5641 ctgagcgcca cgccgaaaat cttcgccggt ggcagcagga gctgattgat gtggagcgtg
     5701 agaataccct tcgacagcac cggtaccagc aacaacggac ggcctgggcc gaacagtatg
     5761 caaactggaa gtttgaagct gaagaacatg aaaaacggct cgccacggct caggcagatg
     5821 cccggcagca gttccggaca gacgccgcgt ttttcgaatc atacctggcg ggtgtgctgg
     5881 cagaaactga atggccgcgt gaaacgcttg ttgcatttga agtaaagccg gagctatcag
     5941 cagtcctgct ggacgttgat ttagctgaga ttgaagattt ccctgataag atttacggcg
     6001 ttaatgcccg gggaacggag ctgacggaaa aagccatgac gcaaaaagcc gtacgcgaaa
     6061 actatgccca ccacgtccat ggctgcttgt tccgcctggt cggtatcgtt ttacatacgc
     6121 tacctttcga caacgtgatt gtgtcaggct ttacgcaacg ggtcagtaag cggaccggct
     6181 atctggagga tgagtatatc ctgtcctgca aatgcactcg cagccagatg tcgtcagtaa
     6241 attttgcagg cataaaacac attgatccgg ttgaagcgtt aggcgatcaa ccggttattc
     6301 gaaagatgag cagtaccttc atttttcagc ctattgaacc actaaccctt taagcaggct
     6361 aaatcttacg atgcaattaa ataccagaca ggcccggatt tttaagcttg ccaacttact
     6421 gggcaccggc aagcccgttt ccgctgccga cattataacc agtctggaat gctctgagcc
     6481 gacattaacc cgggccctca aagagctacg cgagtcatat tctgcggaga tcaaatacag
     6541 caaggcaggt cattcctatc atctcgttaa tccgggccag ttggataaaa agaccctccg
     6601 ccggatgaat gaagcgctcg cacagaatgc tgaactgaaa accggtgagt ccacagggaa
     6661 ggtcgtactc gataaggata aaaaaacagc cgtgtcatta tcgctacgca tgcggattct
     6721 aagaaaaatt gaccgtctgg ctgcgctgag tgactcgacc cgaagtgaag cggtagagaa
     6781 gctggctctg cactccgttg atgaactgat aaaagaatac agcgctaaaa agtcctgacg
     6841 ctaaatctca gaccggggtg aaaactccgg ttttgtcata tttcacctct gtattggcgt
     6901 gatgcaaaat ggcatctgaa tcgatcatgg tttggcatcc atttccgatc acagtttggc
     6961 atcacaatcg atcacgattt ggcatgcttc cgctcattga ttggcatcct ggtgtcgtta
     7021 cggggaagtc atcttcacag ggatcttcag tcagttagca ttaccgacat ccattcacca
     7081 acttaccgga gataatcaat gtcaaaaccc cgatggacgc ttgatcagaa aaaacatcac
     7141 gttgccgcat ggcgcgccag cggccttacc cgtgaacagt actgtgaact ttacgacatt
     7201 ccgtttaaat ccctgcgcca gtggccgcag gatgtcgcaa aagccgaaaa acgagccaga
     7261 gcaccagaaa ttattcctgt ttcagtatct ggctcttccg gaatgactga tggcagacca
     7321 ttgtcggatg agcctgtcac tctcttcctg cccggtggta tccggatgtg ctgccagcct
     7381 tctcagttaa ccgatgtttt ccgggctctt cgccatgctg acgcctgata acgtattcct
     7441 ggtcgtaaaa cccgtcgata tgcgccgggg tatcgacaca ctgacacagt acgtccagaa
     7501 cgaacttaac gctgcatggc atgacggtgc tgcttttgtc ttcaccaata aagtccgctc
     7561 ccgcataaaa gttctccggt gggacaaaca tggcgtatgg ctgtgtaccc gacgactgca
     7621 ccggggaagc ttccgctggc cccggaaagg tgatgccaca tggcatctca cacaggatga
     7681 gtttcactgg ctggtctttg gggttgactg gcaacaggtg aaagggcatg acctggcaaa
     7741 atgggtatac caggaagaac ccgaagatgc tcagagcaac gcaataactc aatgaaatat
     7801 aatgaatatc catgcgtgat gtcatataat ccaccgcatg gatatctcgc ttctcagtac
     7861 caccagtgac cctgaacagc ttcgcgctct ggcaattgcc atggtgcaaa aagtcatggc
     7921 tgagaatgca gaacttcaga accgtatccg catcctggaa gagcagatga agctcgcacg
     7981 ccagcagcgt ttcggtaaaa aatgcgaatc gctggccggg atgcagcgtt ccctgttcga
     8041 agaggatgtt gacgcggata tcgcagaaat ctcggcgcat ctggacaaac tgcttcctca
     8101 gaccggggac gaagaaaaaa caacaacacg tcctgttcgt aagcccttgc cttcacctct
     8161 tccccgtgct gaaaaggtta tcccgccagc tgaggaacgt tgtcctgact gtgatgcccc
     8221 cctgcacttc attcgcgatg aagtcagcga gaagctggaa tatattccgg cacaggtcgt
     8281 ggtcaaccgc tatatccgcc cgcagtacag ctgtccctgc tgtgagaaag tgttcagcgg
     8341 aaaaatgccg gcacatatcc ttccgaaaag tgctgttgag ccatcggtca tcgcacaggt
     8401 tgtcatcagc aaatataccg accatctgcc cctgtatcgc cagcagcaca ttttctcccg
     8461 aatgggggtg gagctccccg tcagtacaat ggcggatatg gtcggtgttg ctggcgctgc
     8521 actggccccg ctggcaaaat tgttacgcca cgagcttctg acacgggatg ttattcatgc
     8581 agatgaaacg tccctgaggc tccttgatac ccggaaaggc ggtaaatcct gctccggctg
     8641 gctgtgggct tacgtcagcg gagaaagagt aagcgtcaac ggagcaccgt attgacgctt
     8701 atttattggt gagtactacg ttccatggca ggagttcatc aactcggttg gaaggccatt
     8761 ctggcagtac gctcaggata tggcgcagat acgcttccgg atcgataccg ttcagacggc
     8821 aggtgccgat cagcccgtac aacagtgctc cacgctcgcc gccgtgatcg ctgccaaaga
     8881 acataaagtt tttctttccg agacagactg cacgaagcgc tctttccgca gcattattat
     8941 ccgcctccgc cagaccgtca tcactgtaat aacagagggc atcccactga ttcagtacat
     9001 agctgcacgc ttcgcccagt ctggattttt tcgacagcgt accattcttc tccaccatcc
     9061 attcatgcag tgacgtcagt aacactttgc ttcgctgctg cctgacggca agacgctctg
     9121 actccggtaa tccccgtatt tcatcctcga tggcgtacag ttcactgatt cgcttcaggg
     9181 cttcttctgc cgtcgcactt ttgctgctga tgtatacatc gtggattttt cgccgggcat
     9241 gggcccagca cgcaacttct gtcagtgcac caccttcacg ttctgcactg aacagccgat
     9301 cgtaaccgct gaacgcatcc gcctgcagga taccccggaa gggacgaagg tgttgtaccg
     9361 gatgttttcc ctgcctgtct ggtgagtagg cgaaccagac cgccggtggc tctgatgagc
     9421 ccgcattccg gtcatcccgg acatacgtcc agatgcgtcc tgtttttgcc ttttttctgc
     9481 ccggtgccag cacttttact ggtgtgtcgt cagtgtgaac cttgcgggtg ttcatcacgt
     9541 aacggtacag ggtatcattc agcagagtca ttaactgaca acatgcgtca acccagttgg
     9601 agagtaatgc ccggctcagt tcgatgccct ggcgggcaaa gatttcactc tgacgataca
     9661 gtggcaggtg ttcgcagtat tttcccgtta acacgcgggc aagtaatccg gagcccgcga
     9721 tgccgcgctc tatcgggcgg gacggcgctg gcgcttcaac tatacagtca cattttgtac
     9781 aggctttttt tacccgaaca gtgcggatca ctttcagggc gctactcacc agttccagct
     9841 gctcagcact gacttccccc agataatcca gctcactgcc acactccggg caacaacttt
     9901 cttcaggctc cagacggtgt atttcacggg gaagatgtgc cggtaacgga cgacgatgac
     9961 gtgactgtcg caactggcgg ggaacctgcg ggtcatcctc acgcccactg taacgatcgc
    10021 tttcctgttc gcgttgtttc agttgggcct cagcctgttc aacctcacgc tgcagttttt
    10081 cagaacgggt accgaacaac atccggcgca gtttttctat ctgggccctc agatgttcta
    10141 tttcccgctc ctcctcttcg atcttttcct cggcacgtgc cagtgcagag cgcaggaagg
    10201 cctccgtctc ttcaaccaga ctcagttgct gatctttctg acggagggct tcagcctgct
    10261 cagagagcag cctttccagc tcagtgatac gaatgaggta tttccgactc atgaccgttt
    10321 ttataatccg gccatgacat ttttacaaca ttgtcagtgc attaaggcgg gatgttttgg
    10381 gctgacgcca gtccagttta tcgaggagca ttgccagctg cgagcgggta atggatacct
    10441 taccgtcacg caccgcaggc cagataaact ggccttcctc cagacgtttg gtgaacaggc
    10501 acagaccatc agcatcagcc cacagaattt taatcgtgtc accccgtcgg ccgcgaaaga
    10561 taaacaggtg accggagaag gggttctcat ccagcacatg ttgtacctgt tctcccagac
    10621 cgttgaagga tttacgcata tcagtaacgc cggcaaccag ccagattcga gtgcctgatg
    10681 ggagcgagat catcgtcccc tcccggtcag ttcacggatc aacaccgtga gcagctctgg
    10741 tgaaggattt tccagcgtca tgttaccgtg gcggaactca actttacagg aactggcact
    10801 gactgtgctt tgtgaaggag tggataaaag cggagtaaga gccgccatag gctctttctg
    10861 ctcatcaggc gttatctcaa caggtaataa ttcaacgcca gcgccagaag aggttgttac
    10921 cggaagacgc cgcgatatac gcctttcgtt ctgccagagc ctgagccatt tgaacaggag
    10981 gttatcattg atatcgtgtt cccgggcaat acgggcaaca gaggctcctg gttgtgaagc
    11041 cagtttaacc atttgaagtt taaactcatt tgaaaatgat ctgcggggtt ctgaggataa
    11101 tattttctgt tccataacag gtgtccacta gttgaaaagg tgggcaccta cgttaccaat
    11161 actggcttaa tggctacata cggcggtcag tttacgctta cgagaaagaa gcggtccgcc
    11221 ggtcgtctgt tttgactcgc agaccggacg ggccctcaga tatcctgagg cctggctgca
    11281 gggctggtgt ggcggtacgc tggtgagtga cggatacagc gtgtacaaat ctctggcgga
    11341 taatgacccc ggtattacat ctgcctgttg ctggagccac gcaaggcgcg gtttcgcaaa
    11401 tctgtataag gccagcaggg agccccgtgc agccatggcg ctgagaaaaa tcgcgggtct
    11461 ttatcgcatt gagaagttca tccgtgaacg tcctgtggaa aaaatacggc aatggcgaca
    11521 acggtattcc cggccgatag tgaatgacct gtttgcctgg cctgaagagc aggagccgtg
    11581 ctgtccgccc gacggaccac taaacaaagc gattaattat atcctgaacc ggcgcgatga
    11641 actgagctgc ttcctgagtg acggagccgt gcccctggac aacaatatct gcgagcgtgc
    11701 tatccgcccg gtggtcatgg ggcggaaagc atggttattc gccggttcac tgatggccgg
    11761 gaaccgcgcg gcacagataa tgagccttct ggaaacggca aaacgtaatg gtctggagcc
    11821 gcatgcctgg ctgacagacg ttctgacgcg tctaccggag tggccggaag accgactgga
    11881 agagttgctg ccccttgagg gatttacctt ctccgggtga gtgatacctg ccatcaggtg
    11941 ttcgtgcagc gggccataac ccgcagtcgg gagttgaacg cctgacgaca ggaaatgagc
    12001 cagagcagcg accacgcggg ctgacagtga gtcagaacct gaatcagccc ggagccattc
    12061 ccggggctgt acttcatcac ctgccatcag tacccgtgtc agtacttcgc gacctgacgc
    12121 aattacccga ctcagttctt cagaacccgc ctccgatacc agcctggcgg tcgccccggc
    12181 gcaacagaat aaatcaacca tcgtcagact gcgatcattc gtatgcatcg ctgatgatgc
    12241 gcccgaaaaa cgcgtcaata tgagttcgcc ggaatattac tgacgacacg gtttgtagtt
    12301 acgttccttc ggtgagtgag tacctgtgcg tcagggtaac gcaatcagtt caggtttagc
    12361 ggggtgtcaa gtcggtttgg ctaaccgacc gcagaattaa ctgtaccttt aaaggtcgcc
    12421 cccgatatat ctgcctgtca gacatatcgc cgattcggta tctcactaca taaaactcct
    12481 taaaaaacaa tgcgattctg ttggtacgaa gctttcagta agtcagtccg gagaagcgcc
    12541 agtacgatac agaatgaggt tgaaaaaaac gttcacgtct ggtcacttct tcaaatgtta
    12601 aaaaacatct cgtaaagagc cagtttatgc gggttcgttg agtagacgtg cgagcgtcac
    12661 tttgtcagct ttaacgctga cgaaagtgac gcccgcacag cttcaaactc cacatgcaca
    12721 cgcaagcgac caaatttcaa atggtcttat gccgtgcgca cagtcataag aagcgcatgc
    12781 ggaaagtcag aaaatatctt aagacgcaga ttttaaaggc gtttttatgc cagtctgccg
    12841 ggaatacaca gtagtccggt tgtccggaag caccacggta atccgaagga aaaatcaacg
    12901 tacctcaatc accgtagtga tatccgttcc cagaggctaa acggatgtac caccataaag
    12961 gcatcctctt tcaggctacg aggatattgc aatcacccga aggcgtttct ctcagattgc
    13021 tgaaacattg gctggcagtc acccgaaggc gtccctctca gactgctgga acattggctg
    13081 gtcgcaaaag cgacagttaa tgcaaataca ggttaacaaa aagggaaatc tgccgtcaac
    13141 ccaaccatat gaacatgttg atttcattat gaatgtgatg ttctaatata aattaatcag
    13201 taacttcata aaaagaatat aagaaacaga taatgcatgt atttacacac atatcagcac
    13261 aatgatagat taccacccca gaaacacgat aaaggtataa ttatggaatt ttccgttatt
    13321 tttttctggg ttatcactgg tcttattacc atcttattct taaaatcaat aataaccgat
    13381 ctaataaaaa acctaaacaa aacagacata gcgttatata tattggcatt tttatcagcc
    13441 ggatatacat tccagtatat ttacaatgac aatatatttt cagaactatg ggg
//
LOCUS       DAZGOB010000136        12787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGOB010000136 DAZGOB010000000
VERSION     DAZGOB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.136"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..766
                     /gene="mngR"
                     /locus_tag="ACHLIF_004134"
     CDS             44..766
                     /gene="mngR"
                     /locus_tag="ACHLIF_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO9608036.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(870..1739)
                     /gene="sucD"
                     /locus_tag="ACHLIF_004135"
     CDS             complement(870..1739)
                     /gene="sucD"
                     /locus_tag="ACHLIF_004135"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO9608037.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(1739..2905)
                     /gene="sucC"
                     /locus_tag="ACHLIF_004136"
     CDS             complement(1739..2905)
                     /gene="sucC"
                     /locus_tag="ACHLIF_004136"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO9608038.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(2999..4216)
                     /gene="odhB"
                     /locus_tag="ACHLIF_004137"
     CDS             complement(2999..4216)
                     /gene="odhB"
                     /locus_tag="ACHLIF_004137"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO9608039.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(4231..7032)
                     /gene="sucA"
                     /locus_tag="ACHLIF_004138"
     CDS             complement(4231..7032)
                     /gene="sucA"
                     /locus_tag="ACHLIF_004138"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO9608040.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(7330..8046)
                     /gene="sdhB"
                     /locus_tag="ACHLIF_004139"
     CDS             complement(7330..8046)
                     /gene="sdhB"
                     /locus_tag="ACHLIF_004139"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO9608041.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYDKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(8062..9828)
                     /gene="sdhA"
                     /locus_tag="ACHLIF_004140"
     CDS             complement(8062..9828)
                     /gene="sdhA"
                     /locus_tag="ACHLIF_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO9608042.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(9828..10175)
                     /gene="sdhD"
                     /locus_tag="ACHLIF_004141"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(9828..10175)
                     /gene="sdhD"
                     /locus_tag="ACHLIF_004141"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO9608043.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(10169..10573)
                     /gene="sdhC"
                     /locus_tag="ACHLIF_004142"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(10169..10573)
                     /gene="sdhC"
                     /locus_tag="ACHLIF_004142"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO9608044.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            10954..11061
                     /locus_tag="ACHLIF_004143"
     CDS             10954..11061
                     /locus_tag="ACHLIF_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608045.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            11267..12550
                     /gene="gltA"
                     /locus_tag="ACHLIF_004144"
     CDS             11267..12550
                     /gene="gltA"
                     /locus_tag="ACHLIF_004144"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO9608046.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         3014 a         3453 c         3235 g         3085 t
ORIGIN      
        1 tatcgggcat aatggctaaa ttcgtattaa tgagatacta aaaatgggac acaagccctt
       61 ataccggcag attgccgatc gcattcgtga acaaattgcg cgtggcgagc ttaaacctgg
      121 cgatgcgtta cctaccgaat cggcattaca aacagagttt ggcgtcagcc gggttacggt
      181 gcgtcaggct ttgcgccagt tagtcgagca acagatcctc gaaagcattc agggcagcgg
      241 gacttacgtc aaagaagagc gggtcaatta cgatattttt cagttaacca gttttgatga
      301 aaaactgtcg gaccgtcacg tcgatacaca cagtgaagtc ctgatatttg aagtgattcc
      361 ggctgacgat tttcttcagc aacagctaca aatcaccgcg caggatcgtg tgtggcatgt
      421 gaagcgcgtg cgctatcgca agcaaaagcc aatggcgctg gaagaaacct ggatgccgct
      481 tgcgttgttc ccggatctca cctggcaggt catggaaaat tcaaaatatc actttattga
      541 agaagtgaag aaaatggtta tcgatcgtag cgaacaggaa atcattcctc tgatgccaac
      601 cgaagagatg agccgtctgc ttaatatcag ccagacaaag ccgattcttg aaaaagtatc
      661 gcgtggatat ttagttgatg gtcgggtgtt tgaatatagc cgcaacgctt ttaataccga
      721 tgactataaa tttaccctga tagcgcaacg aaaatcatcg cgataagcac aaaaaaggcc
      781 atcatacgat ggccaaccat gtcgaaaccg acagcccttc gccattcctg tagagggaag
      841 cggcgagggc tatttcttat tacagatatt tatttcagaa cagttttcag tgcttcaccg
      901 atatccgcca ggctgcgaac ggttttcacg cctgcggctt ccagagcagc gaatttctca
      961 tccgcagtcc ctttcccacc ggcaatgatg gcacccgcgt ggcccatacg tttgcctttc
     1021 ggcgcagtca caccagcgat gtaacccaca actggcttgg taacatgctc tttgatgtac
     1081 gctgccgctt cttcttcagc gctaccgccg atctcaccga tcattacgat cgcttcggtc
     1141 tgcggatctt tttcgaacat ttcgagaatg tcgataaagt tagagccagg gatcgggtca
     1201 ccgccgatac caacgcaggt cgactgaccg aaaccgtaat ccgtggtctg tttaaccgct
     1261 tcataggtca gtgtaccgga acgggaaacg atacccactt tacccggttt gtgaatgtga
     1321 cccggctgga taccgatttt gcattcaccc ggagtgataa cgcccgggca gttcgggccg
     1381 atcatacgaa cgccggcttc gtccagtttc actttcacag tcagcatatc cagcgtcggg
     1441 atgccttcag tgatggtgat aatcagtttg atgcctgcat caatagcttc cagaatggag
     1501 tctttgcaga acggtgctgg tacgtagata acagaagcgg tagcgccagt agcagcaaca
     1561 gcttcacgca cggtgttgaa caccggcagg ccgaggtggg tagtaccgcc tttacctggg
     1621 gttacgccgc caaccatttt agtgccgtat gcaatggcct gttctgagtg gaaagtcccc
     1681 tggctaccag taaagccctg gcagataacc ttggtgtttt tatcaattaa aatggacatt
     1741 atttcccctc cactgcggca acaacctgct gagctgcatc cgtcagacct tttgctgcaa
     1801 taatattcag gccgctgtca gccagtttct tcgcgccgag ttcggcgttg ttaccttcca
     1861 gacgtaccac gaccggtacg ttaacaccca cttctgctac cgcgccgatg ataccgtcag
     1921 cgatcaggtc gcaacgaacg ataccgccga agatgttaac cagaacggct ttcactttgt
     1981 cgtcagagag gatgattttg aacgcttcgg ttacacgttc tttggttgcg ccgccgccaa
     2041 cgtcaaggaa gttagccggt tcgccgccgt gcagtttaac gatgtccatc gtacccatcg
     2101 ccagacctgc gccgttaacc atacaaccga tgttaccgtc cagcgcaacg tagttcagtt
     2161 cccactgtgc agcctgtgct tcacgcggat cttcctgcga ctggtcacgc atttcgcgca
     2221 gatcaggctg gcggaacagt gcgttgccgt cagcgcccag tttgccgtcg aggcaaatca
     2281 gatcgccctg tttggtgatg accagcgggt tgatttcgat caacgccagg tcgcgctcca
     2341 ggaaaatggt cgccaggccc atgaagattt tggtgaactg ctgaaccagt ttaccttcca
     2401 gacccagttt gaacgccagc tcgcgtccct gatacggcat cgggccagtc agcggatcaa
     2461 gcgcaacttt gtggatcagg tgcggagttt cttccgccac tttttcgatt tccacgccgc
     2521 cttcggtgga ggccataaag accacacgac gggaactacg gtctacaacg gcaccgagat
     2581 acagctcttt agcgatatcg gtcgctgctt caaccagaat ctggttaacc ggttggccat
     2641 tggcatctgt ttgatacgtt accagacgct tgccgagcca gttttctgca aaagcacgga
     2701 tgtcttcttt gctgtttaca actttcacac cgcccgcttt accgcggcca ccagcgtgaa
     2761 cctgacattt cactacccac gggccggcac cgatttttga agcggcttct tctgcttcgc
     2821 gcggagtagt acaggcataa cccaccggtg ctggtaagcc atagcgggca aaaagttgtt
     2881 ttgcctgata ttcatgtaag ttcatgtgtt ctgtccatcc ttcagtaatc gttatctttt
     2941 aaaccgtaga ccgacaccct atccatgtcg gtctacagtg caggtgaagc ttaaactact
     3001 acacgtccag cagcagacgc gtcggatctt ccagcaactc tttgatcgtt accaggaagc
     3061 ccacggattc gcgaccatcg atcagacggt gatcgtagga cagcgccagg tacatcatcg
     3121 gcaggatctc aacctgacca ttcaccgcca tcggacgatc tttgatagcg tgcataccca
     3181 gaattgcgct ctgcggcggg ttgatgatcg gcgtagacat cagggaaccg aacacaccac
     3241 cgttggtgat ggtgaagtta ccaccggtca gatcttcaac ggtcagcttg ccgtcacggc
     3301 ctttgactgc cagctctttg attttcttct cgatgtctgc catgccgagg gtatcgacat
     3361 cacgcagtac cggtgtcacc aggccgcgcg gcgtagaaac cgccatgctg acgtcgaaat
     3421 agttgtggta aaccacgtca tcgccgtcga tagacgcgtt cacttccggg taacgtttca
     3481 gggcttcaac caccgctttc acgtagaagg acataaagcc cagacggatg ccgtggcgtt
     3541 tttcaaacgc ttcaccgtac tgcttacgca gatccataat cggcttcatg ttgacttcgt
     3601 tgaacgtggt cagcatggcg gtggagtttt tcgcttccag cagacgctct gccacacgct
     3661 tacgcaggcg agtcatcggg acacgttttt cactacgtgc agccagagcc ggttgcgccg
     3721 ccggagcagc cgctgccgga gcagactctt tcgccggggc tttcgccaga tgtttttcca
     3781 catcttcacg agtcagacga ccacccacac cggtgccttt aatggcgctg gcgtcgagat
     3841 tgtgttcagc cagcaggcga cggatcgccg ggcttaacgc atcgttgttt tgctcttcca
     3901 gagatgcctg ctggcgttgc gccggagtgg acgctttctc ttcagatttg gcgctggttt
     3961 ctttaccggc gctgttgcct tcacgcaggc gaccaaggat ctgacgagac gttaccgttg
     4021 taccttcatc ttccagaacc gcatccagaa tgccgtctgc tgatgccggt acttccagta
     4081 ccactttgtc agtttcgatt tctaccagca cttcatcacg tacgactgcg tcgccgggtt
     4141 ttttatgcca ggttgcgacg gtggcatcgg ctacggattc aggcaggtca gggaccagaa
     4201 tatctacgct actcattgtg tatcctttat ttattcgacg ttcagcgcgt cattaaccag
     4261 atcttgttgc tgtttctggt gaacggacat ataccctacc gccggagagg cggaggccgg
     4321 gcggcctgca taacgcagag aagccccaaa cggaatcact tcacggaaat gatgctggct
     4381 gcagtaccat gcgccctggt tgagcggctc ttcctggcac cagacaaaat ccttgacgtg
     4441 agcaaactgc tgcaacactt cctgcatcgc tttatgcggg aacgggtaga gttgctcgat
     4501 acgcacaatg gcgacatcgt gttgattgtt cttacgacgc tgttccagca ggtcgtaata
     4561 aaccttacca gaacacatca ctacgcgctt cacgcccttc ggatcaagct cgtcgatttc
     4621 accgatggct ggcaggaagg tgccgttcgc cagttcttcg aggctggaaa ccgccagcgg
     4681 atgacgcagc agggatttcg gcgacatcac gaccagcgga cgacgcatcc cgcgcagcgc
     4741 ctgacgacgc agcatgtggt aaacctgtgc cggggtagac ggtacgcaca cctgcatgtt
     4801 ttgctcagca caaagttgca gataacgttc cagacgcgcg gaggagtgct ccggcccctg
     4861 cccttcgtaa ccgtgcggca gcaacatcac cagaccacac atccggcccc atttctgttc
     4921 gccagaagag atgaactggt cgataaccac ctgcgcaccg ttggcgaagt caccgaactg
     4981 cgcttcccag atggtcagag tgcgtggttc tgcggtggca taaccgtatt caaacgccag
     5041 cactgcttct tcagacagta cggagtccca gacgcggaac gcgccctgcc cgttatggat
     5101 gtgttgcaac ggcgtgtaag tggaaccgtt agactggttg tggatcaccg cgtggcggtg
     5161 gaagaaggta ccgcgaccgg agtcttcacc cgacaggcga accggaatgc cttcgtcaac
     5221 cagcgtggcg taagcgaggt tttccgcacc gccccagtcg aacagtttct caccggcagc
     5281 catcgcctgg cgatcgccat aaatcttggc aacgcgagac tgcatttcaa ctgcttccgg
     5341 caccgtgctg atgcgtttcg ccagctcctg caggcgcttc atctcaactt tgttcgggta
     5401 ctcttcgtcc cattcgtggt tgaggtacgg cgaccaggtg aaagagtgca tgttcatcgg
     5461 acgccactct gccactacgc aatcgccagc atccagcgca tcgcggtaca ggttaaccat
     5521 ctcggtggca tcttccagcg tcgccacttt ttcctgctcc agcttgtcag cgtagatttt
     5581 gcgcggtgtc ggatgttttt tgattttctg atacatcagc ggctgggttg cgctcggctc
     5641 gtcggcttcg ttgtggccgt gacggcggta gcacaccagg tcgatgaaga catcacgttt
     5701 aaaggtgtta cggaaatcga gcgccagacg ggtcacaaag gcaacggctt ccggatcatc
     5761 cgcgttaacg tggaaaatcg gtgcctgaac catcttaccg atatcagtac agtacggtgt
     5821 agaacgagca tccagcggat tagaggtggt gaaaccaacc tggttgttga taacgatacg
     5881 taccgtaccg ccaacttcat aaccacgcgc tttcgacatg ttcagagttt cctgaaccac
     5941 gccctgcccg gtcactgcgg cgtcaccgtg gatggtgatt ggcagcactt tgttgctgct
     6001 cggctcatca agtctgtcca gacgggcacg aacagaaccg ataactaccg ggcttacaat
     6061 ctcaaggtga gacgggttaa acgccagcgc caggtgcacc aggccgccat cggtctggaa
     6121 gtcagacgag aagcccatgt ggtatttcac gtcacccgtg ccgaggtgtt ctttatgttt
     6181 accggcaaac tcgtcgaaca agtcttgcgg ttttttaccc agcacgttca ccagcacgtt
     6241 cagacgacca cggtgcgcca tcccaagaac cacttcgcgg gtgccgctgt tgccagcgtg
     6301 gcggatcatc tctttaagca tcgggattaa cgcgtcaccg ccttccagcg agaagcgttt
     6361 tgcgccaggg aattttgcgc cgaggtaacg ttcaaggcct tcagcggcgg tcagttcgct
     6421 taagaagcgt tttttctctt cgctattgaa agtcgcgcga ccagactcga tacgctgttg
     6481 gatccagcgt ttttcttcgg tgctggtaat gtgcatatac tcggcaccaa tcgggccaca
     6541 gtaggtttgc ttgagggctt ccagcagctc gccgagtttc atggtttctt tgccgctggc
     6601 aaatgaaccg acgttgaagg tctcctggaa gtctgcttcg gtcagatcgt ggaaagacgg
     6661 atccagatcg gccactttat cttgctgcca cagtcccagc ggatcgagat tcgcatgctg
     6721 gtgaccacgg aagcggtatg cgttaatgag ctgcaggact ttaacctgct tcacattggt
     6781 gtcagggtcg gagatcgttg aagagtaacg tgaagcgtct ttcgccaggc ggcggaaata
     6841 ttcacgcgtt tgagagtgga attgatccgg tttgactccc gtaccaggta actgctggaa
     6901 cgtcgaacgc cagttagcgt caaccgagtc aggatcggtt aagaagtctt catagagctg
     6961 ttctatccag ctctggtttg cgccagagag gtaagaagag tccaaccagg ctttcaaagc
     7021 gctgttctgc atcgtgatcc cttaagcatc ttttttatgc ttacttcgcc gtggatacta
     7081 ccacgcacag tgatgtgcgt gccggggttc acttgcgagc tcttgtctat gtagtggctc
     7141 gcgaaggaac ctttaaaaac tgtcttagtg tcaggggcag tttttaaaga tttcctgcat
     7201 ttctttgtta cgcctgatgc gctgcgctta tcaggccggg taatgacttg taggccggat
     7261 aaggcgttca cgccgcatcc ggcactggtt gcctgatgcg acgcttgcgc gtcttatcag
     7321 gcctacggtt tacgcattac gttgcaacaa catcgactta atatggccga tggcgcgcgt
     7381 cgggttcagc cccttcggac atacactgac gcagttcatg atgctgtgac agcggaatac
     7441 gctgaatgca tcgctcaaac cgtcgaggcg gctgtcagtc tcggtatcac ggctatcgat
     7501 caggaaacga tatgccgcta acaagcctgc cgggccgata aacttatcgg gattccacca
     7561 gaaagacgga caagaggttg aacaacatgc gcagagaata cattcataca gcccgtcgag
     7621 tttttcgcgc tgctctggca tctgtaaatg ctcgcgagct ggcggatttt gtccattatt
     7681 caacaggtaa ggcttaattt tgtcatattg cgcatagaat tgtcccatgt ctaccaccaa
     7741 atcgcggatc accggtaaac ctggcagcgg gcgaatcaca atcttcttgc ccggctggtt
     7801 gagtgccgaa atcggggtaa tacaggccag accattcttg ccgttcatgt tcagaccgtc
     7861 ggaaccgcac acaccttcac ggcaggagcg gcggaacgac aggctgggat ctttctcttt
     7921 cagctggata agcgcatcca gcagcatcat gtcgcgacct tcttccgctt ccagggtgta
     7981 atcctgcata cgcggagcat catcaacatc cgggttatag cgataaattg aaaactcgag
     8041 tctcattttc ctgtctccgc attagtaagt acgaatcttc ggcgggaatg ccgggcgcag
     8101 tttcggttcc atgttgacgc ttcggcgcgt catggattcc gactctggca gatacaggga
     8161 gtggcacagc cagttttcat catcacgatc cgggaagtcg aagcggctat gtgcgccacg
     8221 gctttcggta cggaagttgg cagaaacagc cgttgcatac gccgtttcca tcaggttatc
     8281 cagttccagg cactcaacgc gctgggtgtt gaactcgctg gaagtgtcat ccagacgggc
     8341 atttttcaga cgctcgcgga tcactttcaa ctgctcaagc cctttcgcca tcgcatcacc
     8401 ttcacggaag accgagaagt tatgctgcat acattcttgc agcgctttac gaatcgccac
     8461 cggatcttca ccgttacgat tattgttcca gcggttcagg cgatccagag acacttcaac
     8521 atcagactcg ctggcatcgc gcagtgcgcc ctgctcggcg atagactctt gcagatgcag
     8581 acctgccgcg cgaccaaaga ccaccaggtc cagcagcgag ttgccgccca gacggttagc
     8641 gccgtgtacc gatacacaag cgatttcacc aacggcaaac agtcccggaa caaccacatc
     8701 ttcgcctttc tcattcacag tcagcgcctg accggtaact ttggtcggaa taccgcccat
     8761 catgtagtga caggttggga taaccggaat cggctctttc accggatcga cgtgagcgaa
     8821 ggtacgggaa agctccagga tacccggcag acgggattcg agaacttctt tacccaggtg
     8881 atcgagtttc agtttcgcgt gtggccccca cggaccatca cagccgcgac cttcacggat
     8941 ttcaatcatg atggaacgcg ccaccacatc acggcccgcc aggtctttgg cgttcggcgc
     9001 ataacgctcc ataaagcgtt cgccatgttt gttcagcaga taaccgcctt caccacggca
     9061 accttcggtg accagtacgc ccgcaccggc aatgccggtc gggtggaatt gccacatttc
     9121 catatcctgc accggtacgc ctgcgcggat agccatgccg acaccgtcgc cagtgttaat
     9181 gtgggcgtta gtggtggact gataaatacg ccctgccccg ccagtcgcca gcacggtagc
     9241 gcgggctttg aaataaacca cttcaccggt ttcgatgcac agtgcggtac aacccaccac
     9301 cgcgccatcc tggtttttca ccagatccag cgcataccac tcggagaaaa tggtggtgtg
     9361 gtttttcagg ttctgctgat aaagcgtgtg caacagtgcg tgaccggtac ggtcagccgc
     9421 cgccgcagtg cgtgccgcct gctcgccgcc gaagtttttc gactgaccgc caaacggacg
     9481 ttgatagata cgaccatcat caagacgcga gaacggcagg cccatgtgtt cgagttccag
     9541 aatcgcttcc ggcccggttt tacacatata ttcaatcgcg tcctggtcac cgatatagtc
     9601 cgaccctttt acggtgtcgt acatatgcca ttcccagtta tcttcatggg tattacccag
     9661 cgcaacggta atgccgcctt gcgcagaaac ggtatgggaa cgggtcggga agactttaga
     9721 gagcagcgca caggtctggc cgctctggga aatttgcagc gccgcgcgca tacctgcgcc
     9781 accggcacca atcacaactg catcaaattc tctgactggc aatttcatca cacaccccac
     9841 accacaacga atccataaat cacgtaaacc accagtgcaa cgacaatcac cagttgcagc
     9901 atcaggcgca atgccagcgg tttaacgtag tcggtcaaca cctgccacat gccgatccag
     9961 gcatgaatca agatagaaaa cagcgccagc agggtgaaca ctttggtgaa cgcagaggcg
    10021 aagaaaccga tccagacttc atatgtcagt tcgccactgg tggcgaaaaa accgaccata
    10081 taaatgatgt agagcgtcag gacgatagcg gtagcgcgaa cgaggatgaa atcatgtacg
    10141 ccattgcgtc ctaatgcgga ggcgttgctt accatacgag gactcctgcg agaagtgaaa
    10201 gcacgacagt aataacaaag gagattttgg cggagcgttt acccgcttcg aatgtttctt
    10261 ccagatagcc aaaatccatc atcatgtggc gaatacctac gacgacgtga tacgccagag
    10321 cggtaaggat gccccacatg ataaatttga cgaagaagct gcccataatc gcggaagctt
    10381 gctcgaaacc ttcaggggaa gagaggctgg tacccagaag ccacagcagg atacccactg
    10441 caataaaggt gatcacaccg gaaacgcgat ggagaatgga cgctatcgcc gtgatgggga
    10501 accggatggt ctgtaggtcc agattaacag gtctttgttt tttcacattt cttatcatga
    10561 ataacgccca catgctgttc ttattattcc ctggggacta cgggcacaga ggttaacttt
    10621 ctgttacctg gagacgtcgg gatttccttc ctccggtctg cttgcgggtc agacagcgtc
    10681 ctttctataa ctgcgcgtca tgcaaaacac tgcttccaga tgcgaaaacg acacgttaca
    10741 acgctgggtg gctcgggatt gcagggtgtt ccggagacct ggcggcagta taggctgttc
    10801 acaaaatcat tacaattaac ctacatatag tttgtcgggt tttatcctga acagtgatcc
    10861 aggtcacgat aataacattt atttaatttt taatcatcta atttgacaat cattcaacaa
    10921 agttgttaca aacattacca ggaaaagcat ataatgcgta aaagttatga agtcggtatt
    10981 tcacctaaga ttaacttatg taacagtgtg gaagtattga ccaattcatt cgggacagtt
    11041 attagtggta gacaagttta ataattcgga ttgctaagta cttgattcgc catttattcg
    11101 tcatcaatgg atcctttacc tgcaagcgcc cagagctctg tacccaggtt ttcccctctt
    11161 tcacagagcg gcgagccaaa taacaaacgg gtaaagccag gttgatgtgc gaaggcaaat
    11221 ttaagttccg gcagtcttac gtaataaggc gctaaggaga ccttaaatgg ctgatacaaa
    11281 agcaaaactc accctcaacg gggacacagc tgttgaactg gatgtgctga aaggcacgct
    11341 gggtcaagat gttattgata tccgtactct cggttcaaaa ggtgtgttca cctttgaccc
    11401 aggcttcact tcaaccgcat cctgcgaatc taaaattact tttattgatg gtgatgaagg
    11461 tattttgctg caccgcggtt tcccgatcga tcagctggcg accgattcta actacctgga
    11521 agtttgttac atcctgctga atggtgaaaa accgactcag gaacagtatg acgaatttaa
    11581 aactacggtg acccgtcata ccatgatcca cgagcagatt acccgtctgt tccacgcttt
    11641 ccgtcgcgac tcacatccaa tggcagtcat gtgtggtatt accggcgcgc tggcggcgtt
    11701 ctatcacgac tcgctggatg ttaacaatcc tcgtcatcgt gaaattgccg cgttccgcct
    11761 gctgtcgaaa atgccgacca tggccgcgat gtgttacaag tattccattg gtcagccatt
    11821 tgtttatccg cgcaacgatc tctcctatgc cggtaacttc ctgaatatga tgttctccac
    11881 gccgtgcgaa ccgtatgaag ttaatccgat tctggaacgt gctatggacc gtattctgat
    11941 cctgcacgct gaccatgaac agaacgcctc tacctccacc gtgcgtaccg ctggctcttc
    12001 gggtgcgaac ccgtttgcct gtatcgcagc aggtattgct tcactgtggg gacctgcgca
    12061 cggtggtgct aacgaagcgg cgctgaaaat gctggaagaa attagctccg ttaaacacat
    12121 tccggaattt gttcgtcgtg cgaaagataa aaatgattct ttccgcctga tgggcttcgg
    12181 tcaccgcgtg tacaaaaatt acgacccgcg cgccaccgta atgcgtgaaa cctgccatga
    12241 agttctgaaa gagctgggca ccaaagatga cctgctggaa gtggctatgg agctggaaaa
    12301 catcgcgctg aacgacccgt actttatcga gaagaaactg tacccgaacg tcgatttcta
    12361 ctctggtatc atcctgaaag cgatgggtat tccgtcttcc atgttcaccg tcattttcgc
    12421 aatggcacgt accgttggct ggatcgccca ctggagcgaa atgcacagtg acggtatgaa
    12481 gattgcccgt ccgcgtcagc tgtatacagg atatgaaaaa cgcgacttta aaagcgatat
    12541 caagcgttaa tggttgattg ctaagttgta aatattttaa cccgccgttc aaatggcggg
    12601 ttaattttta tatgcctaaa cacaaaaaat tgtaaaaata aaatccacta tcggacattc
    12661 atatattcta aaaaaaaata tatcggtata aattataaac aaatcgatga tttttaatca
    12721 tctatcttcc tctcagttac atgaataaac caccaaatat agtgcacccc tataatttcc
    12781 aatactt
//
LOCUS       DAZGOB010000137        12617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGOB010000137 DAZGOB010000000
VERSION     DAZGOB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.137"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            56..1570
                     /gene="caiT"
                     /locus_tag="ACHLIF_004145"
     CDS             56..1570
                     /gene="caiT"
                     /locus_tag="ACHLIF_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO9608047.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1601..2743
                     /gene="caiA"
                     /locus_tag="ACHLIF_004146"
     CDS             1601..2743
                     /gene="caiA"
                     /locus_tag="ACHLIF_004146"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO9608048.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2872..4089
                     /gene="caiB"
                     /locus_tag="ACHLIF_004147"
     CDS             2872..4089
                     /gene="caiB"
                     /locus_tag="ACHLIF_004147"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO9608049.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4163..5716
                     /gene="caiC"
                     /locus_tag="ACHLIF_004148"
     CDS             4163..5716
                     /gene="caiC"
                     /locus_tag="ACHLIF_004148"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO9608050.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5825..6610
                     /gene="caiD"
                     /locus_tag="ACHLIF_004149"
     CDS             5825..6610
                     /gene="caiD"
                     /locus_tag="ACHLIF_004149"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO9608051.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6616..7206
                     /gene="caiE"
                     /locus_tag="ACHLIF_004150"
     CDS             6616..7206
                     /gene="caiE"
                     /locus_tag="ACHLIF_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO9608052.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7292..7687)
                     /gene="caiF"
                     /locus_tag="ACHLIF_004151"
     CDS             complement(7292..7687)
                     /gene="caiF"
                     /locus_tag="ACHLIF_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO9608053.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7948..11169)
                     /gene="carB"
                     /locus_tag="ACHLIF_004152"
     CDS             complement(7948..11169)
                     /gene="carB"
                     /locus_tag="ACHLIF_004152"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO9608054.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11187..12335)
                     /gene="carA"
                     /locus_tag="ACHLIF_004153"
     CDS             complement(11187..12335)
                     /gene="carA"
                     /locus_tag="ACHLIF_004153"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO9608055.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2937 a         3286 c         3360 g         3034 t
ORIGIN      
        1 aataaaacaa acgggaacgc aaaataaata ttcgttttca cagtggaatt aactcatgaa
       61 gaatgaaaag agaaaaacgg gaatagaacc gaaggttttc tttccgccgt taataatcgt
      121 cggcatactt tgttggctta cagtcagaga tctggatgca gcgaatgtcg ttattaatgc
      181 tgtattcagt tacgtcacca atgtatgggg atgggcattt gaatggtata tggtggtgat
      241 gcttttcggt tggttctggc tggtgtttgg cccgtatgcc aaaaagcgtt taggtaacga
      301 accacctgaa tttagtaccg ccagttggat ctttatgatg ttcgcctcct gtacgtctgc
      361 tgccgtactg ttctggggat cgattgagat ctactactac atctccaccc cgccgtttgg
      421 cttagaaccg aactcgacag gggcgaaaga gttggggctg gcttacagct tgttccactg
      481 gggacctctg ccgtgggcca cttacagctt cctttcagtc gccttcgctt acttcttctt
      541 tgtccgcaaa atggaagtga ttcgccccag ctcgacactg gtgccgctgg taggtgaaaa
      601 acacgccaaa gggttgttcg gcactatcgt cgacaacttc tatctcgtcg ccttgatctt
      661 cgcgatgggt accagtctgg gccttgccac gccgctggtg accgagtgta tgcaatggtt
      721 gtttggcatt ccgcataccc tgcaactgga cgctatcatc attacctgct ggattatcct
      781 caacgccatt tgcgtcgctt gcggtctgca aaaaggggta cgtatcgcca gtgacgtgcg
      841 tagttacctg agcttcctga tgctgggttg ggtgttcatt gtcagcggtg ccagcttcat
      901 catgaactac ttcaccgatt cggtggggat gttgctgatg tatctgccgc gcatgttgtt
      961 ctataccgat cccatcgcta aaggcggctt cccgcagggc tggaccgtgt tctactgggc
     1021 atggtgggtg atttatgcta tccagatgag tatcttcctc gcccgcatct cccgtggtcg
     1081 tactgtgcgt gaactgtgct tcggcatggt gctggggctg acagcgtcaa cctggatcct
     1141 gtggactgta ctcggtagta acactctgct gttgatagat aaaaacatca tcaacattcc
     1201 aaatctgatc gaacagtacg gtgtggcgcg cgccatcatt gaaacctggg ccgctctgcc
     1261 actcagcacc gccaccatgt ggggcttctt catcctctgc tttattgcca ccgttacgct
     1321 ggttaacgcc tgctcttata ccctggcgat gtccacttgc cgcgaagtac gcgatggtga
     1381 agaaccacct ctgctggtgc gtatcggttg gtcaattctg gttggcatta tcggtattgt
     1441 tctgctggcg ctcggcggcc tgaaaccgat tcaaaccgcc attatcgccg gaggatgccc
     1501 gctgttcttc gtcaacatta tggtgacgct ctcctttatt aaagacgcga aacagaactg
     1561 gaaagattaa ttaaccccca aaatatcaag aggttgaaag atggatttta atttaaatga
     1621 tgagcaggaa ctgtttgtcg ccggtatccg cgaactgatg gccagcgaaa actgggaggc
     1681 ctattttgcc gagtgcgacc gtgacagcgt ctacccggaa cgttttgtca aagcactggc
     1741 ggatatgggt atcgacagtc tgctgatccc tgaagagcac ggtggtctgg acgcggggtt
     1801 tgttactctc gccgccgtgt ggatggagct gggacgtctg ggggcaccaa cctatgtgct
     1861 gtaccagttg ccgggcgggt tcaacacctt cctgcgcgaa ggcacacaag agcagatcga
     1921 caagattatg gctttccgcg gcaccggtaa gcagatgtgg aactcagcga ttaccgaacc
     1981 gggcgcgggc tccgacgtgg gtagcctgaa aacgacttat acccgtagaa atggtaagat
     2041 ttatcttaat ggtagtaagt gttttattac cagcagcgcc tacaccccgt acatcgtggt
     2101 gatggcgcgc gacggggctt ctccggacaa acctgtctac accgaatggt ttgttgatat
     2161 gagcaaaccg ggcatcaaag tgaccaaact tgaaaagctc ggtctgcgta tggatagctg
     2221 ctgtgaaatc acctttgacg acgtggaact ggacgagaaa gacatgttcg gtcgggaagg
     2281 taacggcttt aaccgcgtca aagaagagtt cgaccatgaa cgtttcctgg tagccctcac
     2341 caactacggt acggcgatgt gcgcctttga agatgcggcg cgctacgcca atcagcgcgt
     2401 gcagtttggc gaggctattg gtcgtttcca gttgattcag gaaaaattcg cccacatggc
     2461 gatcaaatta aactccatga aaaacatgct gtatgaagca gcgtggaaag cagacaacgg
     2521 caccatcacc tctggcgatg cagcgatgtg caaatacttc tgcgccaatg cggcatttga
     2581 agttgtggat agcgcaatgc aggtgctggg cggtgtcggg attgcgggca accaccgcat
     2641 cagccgcttc tggcgtgatc tgcgtgtaga ccgcgtctcc gggggatctg acgaaatgca
     2701 gatcctgacg ctgggtcgcg cggtgctgaa gcaataccgc taagtccgtg cgctgcctga
     2761 tgcgacgcta atgcgtctta tcaggcctac gggagcacga atgtaggccg gataaagcgt
     2821 ttacgccgca tccggcagtc atgcgctaca taaatttttc aggagctaat aatggatcat
     2881 ctacccatgc cgaaattcgg gccgttggcc ggattgcgcg ttgtcttctc cggtatcgaa
     2941 atcgccgggc cgttcgccgg gcaaatgttc gcagaatggg gtgcggaagt tatctggatc
     3001 gagaacgtcg cctgggccga caccattcgc gttcaaccga actacccgca gctctcccgc
     3061 cgcaatttgc acgcgctgtc gttaaatatt ttcaaagatg aaggccgcga agcgtttctg
     3121 aaattaatgg aaaccaccga tatcttcatc gaagccagta aaggtccggc ctttgcccgt
     3181 cgtggcatta ccgatgaagt actgtggcag cacaacccga aactggttat cgctcacctg
     3241 tccggttttg gtcagtacgg caccgaggag tacaccaatc ttccggccta taacaccatc
     3301 gcccaggcct tcagtggtta cctgattcag aacggtgatg ttgaccagcc aatgcctgcc
     3361 ttcccgtata ccgccgatta cttttctggc ctgaccgcca ccacggcggc gctggcagca
     3421 ctgcataaag tgcgtgaaac cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg
     3481 atgctgcgta tgggccagta cttcatgatg gattacttca acggcggcga aatgtgcccg
     3541 cgcatgagca aaggtaaaga tccctactac gccggttgcg gtctgtataa atgcgccgac
     3601 ggctacatcg tgatggaact ggtgggcatt acccaaattg aagagtgctt taaagatatt
     3661 ggcctcgcac atctgctcgg tacgccggaa atcccggaag gcactcagct tatccaccgt
     3721 atcgaatgcc cttacggccc actggtagaa gagaaactcg atgcctggct ggcggcacat
     3781 accatcgcag aagttaaaga acgctttgcc gaactgaata tcgcctgtgc caaagtactg
     3841 accgtaccgg aactggaaag caatccacag tatgttgccc gtgaatcaat cactcagtgg
     3901 caaacgatgg atggtcgcac ctgcaaaggg ccgaacatca tgccgaaatt caaaaataac
     3961 cccggacaaa tctggcgcgg aatgccctca catggcatgg acacggctgc cattttgaaa
     4021 aatatcggct acagcgaaaa cgacattcag gagttggtca gcaaaggtct ggccaaagtt
     4081 gaggactaaa cgcgtggcgt cagtcggctt gcctgactag cgcccgccca ataacaaacg
     4141 aacaaaaatg gatagaggtg caatggatat cattggcgga caacatctac gtcaaatgtg
     4201 ggacgatctg gcggacgttt acggtcataa aacggcgctg atttgtgaat ccagcggcgg
     4261 agtcgttaac cggtatagtt atcttgagtt aaatcaggag attaaccgca cggcaaacct
     4321 gttttatacg ctggggattc gcaaaggcga caaggttgca ctacatctcg acaactgccc
     4381 ggaatttatc ttttgctggt tcgggctggc aaaaattggc gcgattatgg tgccgattaa
     4441 cgcccgcctg ttacgcgagg aaagcgcgtg gatcctgcaa aatagccagg cgtgcctgct
     4501 ggtgaccagt gcgcaattct atcctatgta tcaacagatt cagcaggaag atgccactca
     4561 attgcggcac atttgcctga cagatgtggc acttcccgct gatgatggcg tgagttcgtt
     4621 tactcaactg aaaaatcaac aacctgccac cttgtgctat gcaccgccgc tattgactga
     4681 cgatacggcg gaaattctct tcacctccgg caccacctcc cgaccgaaag gtgtggtgat
     4741 tacccattac aacctgcgct tcgctggata ttactccgcc tggcagtgtg cactgcgtga
     4801 cgatgacgtc tacctgacgg taatgcctgc gtttcatatc gattgccagt gtactgcggc
     4861 gatggcggcg ttttctgccg gggccacctt tgtgctggtc gagaaataca gcgcccgcgc
     4921 cttctgggga caggtgcaga agtaccgcgc caccattacc gaatgtattc cgatgatgat
     4981 ccgtacgttg atggtgcagc cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt
     5041 gatgttttat ctcaacttgt cggagcagga aaaagatgcg ttttgtgaac gcttcggcgt
     5101 tcgcttgctg acgtcttatg ggatgacgga aaccattgtg ggcattatcg gcgatcgccc
     5161 tggcgataaa cgacgctggc cgtcgattgg tcgggcgggg ttttgctacg aagcggagat
     5221 ccgcgacgat cacaatcgcc cgctcccggc aggtgagatc ggtgaaatct gtattaaagg
     5281 cgtaccaggg aaaaccatct tcaaagagta ttttctcaac ccgaaagcca ctgcgaaagt
     5341 gctggaagcc gacggctggc tacataccgg cgataccgga tactgcgacg aagagggctt
     5401 tttttatttc gtcgatcgcc gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg
     5461 cgtggagctg gaaaatatca tcgccaccca tccaaaaatt caggatatcg tggttgtggg
     5521 tattaaagat tcgattcgcg atgaagccat caaagcattt gtggtgctga atgaaggtga
     5581 aacattgagc gaagaggaat ttttccgctt ctgcgaacaa aatatggcga aatttaaagt
     5641 gccctcttat ctggagatca gaaaagatct gccacgtaat tgctcgggga aaataattag
     5701 aaagaatctg aaataaatga aacagcaggg aactaccctg ccagctaata atcacgctat
     5761 aaaacaatat gcattttttg cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa
     5821 aggaatgagt gaatcattac atctgacccg caatggatca attctggaaa ttacccttga
     5881 tcgtccaaaa gcgaatgcta ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct
     5941 aaatttccgt gacgatccgc aattacgtgt cgccattatt accggtgccg gagagaagtt
     6001 cttttccgcg ggctgggatt taaaagcggc agcagaaggc gaagcaccgg atgctgactt
     6061 tggcccgggt ggatttgcgg gattaaccga aattttcaat ctcgacaaac cggttatcgc
     6121 agctgtgaac ggctatgcct ttggcggcgg tttcgaactg gcgctggcgg cagattttat
     6181 tgtttgtgcc gataacgcca gcttcgccct gccggaagcc aagctgggca tcgttcctga
     6241 cagcggcggc gtgctgcgtc taccgaagat cctgccgcct gccatcgtca atgaaatggt
     6301 gatgaccggc agacgaatgg gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt
     6361 ggttagccag gcggaactga tggataacgc ccgcgaactg gcgcagcagc tggttaacag
     6421 cgccccgctg gcgattgcgg cgctgaaaga gatcttccgc accaccagcg aaatgccggt
     6481 agaagaagcg tatcgctata ttcgcagcgg cgtgttgaaa cactatccgt cggttctgca
     6541 ttcggaggat gccattgaag ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa
     6601 aggacgttaa caaccgtgag ctattacgcc tttgaggggt taattccggt ggttcacccg
     6661 acggcgtttg tccatcccag tgccgtcttg attggcgatg tgattgtggg agccggtgtc
     6721 tacatcggcc cactcgcctc actgcgtggt gactacgggc ggttgatcgt gcaagcggga
     6781 gccaatattc aggatggctg cattatgcat ggctactgcg acactgacac catcgttggg
     6841 gaaaacggcc atatcgggca cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca
     6901 ttggtcggga tgaacagcgt gattatggat ggcgcggtca ttggcgaaga gagcattgtt
     6961 gccgccatga gctttgtcaa agcaggcttt cacggcgaga aacgccagtt gttgatgggt
     7021 acgccagccc gcgctgtacg cagtgttagt gacgacgagt tacactggaa acggttgaat
     7081 accaaagagt atcaggatct tgtcgggcgc tgtcatgcat cgttacatga aacacagcca
     7141 ctgaggcaaa tggaggaaaa tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa
     7201 cggtgatctt gccggatgcg acctggttgc tggataagat gctacaacat cgcatctggc
     7261 aagcaggacg cccggtatca acgagataaa attaacgacg catactcttt gacaacatca
     7321 tctgccactt ctgctcgcgg ttaagttccg tgagcggaac ggcaggaata cgcggcgtct
     7381 ttcttacgcc gactaattta tcccggctgt tattgcgcag gcgcgcgtaa atttgctcat
     7441 cgatatcgac cacttttacc agtcgctggc actggcatcc ccgtccttcc agcttattag
     7501 ggatcatctt aacttcgcag cttatttctg cgacttccga cagaatataa gtcagggtat
     7561 taaccgcctt gctgtgttca atatcgaaat gaatagagat ctctcttgct atcacccacc
     7621 gattttcagc catcatccat tcggcgatta acaagtagag tggtttttca acatatcctt
     7681 cacacatacg aactccagtt tcacaaatta aaagtctggc tatgactcgt tttgaagcaa
     7741 ttaaacaagg ccatattaaa attgtggata tatcgatagt atgcaacttc accgtcgata
     7801 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttactta
     7861 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
     7921 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
     7981 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
     8041 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
     8101 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
     8161 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
     8221 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
     8281 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
     8341 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
     8401 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
     8461 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
     8521 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
     8581 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
     8641 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
     8701 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
     8761 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     8821 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     8881 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     8941 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     9001 ccgaccgccg agaacgtaag acggacgtac caccagcggg tagccaatct ctttcgcctt
     9061 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     9121 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     9181 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     9241 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcta gcacatcttc
     9301 cagtgttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     9361 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagtgcca gcgaggcgtg
     9421 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     9481 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     9541 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     9601 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     9661 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     9721 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     9781 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     9841 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     9901 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     9961 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
    10021 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
    10081 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
    10141 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
    10201 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
    10261 gcgcgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
    10321 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
    10381 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
    10441 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
    10501 atcacgcacc acttccatct cgtactcttt ccagccaatc agcgactcat caatcagcaa
    10561 ctctttggtc ggagagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
    10621 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
    10681 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
    10741 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
    10801 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
    10861 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
    10921 cgggcgctct ttttcaataa tcttgcgtac cacttcccag tgaatcggct cgatgtaggt
    10981 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
    11041 ccggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
    11101 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
    11161 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
    11221 tcgataaagt ggtcgaacaa cggcgcggcg tcgtgtggac cagggctggc ttcagggtgc
    11281 ccctggaagc tgaacgccgg tttatcggtg cgatgaatgc cctgtaacgt accgtcgaac
    11341 agggatttat gcgtgacacg caggtttgct ggtaatgttg cttcgtccac cgcaaaaccg
    11401 tggttctggg cggtgatcat caccacgttt ttctcaacat ctttaaccgg atggttgccg
    11461 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
    11521 tgaccgaggc agatgccgaa gaccggaata tcggtttcga ggaatttctg gatggcggta
    11581 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat accgtctgga
    11641 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
    11701 ctgtccacca acatgcgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
    11761 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
    11821 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
    11881 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
    11941 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
    12001 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
    12061 aagttgctgg caatcagcgg caggtcgcga atcaccagac cttgtgcatg tacctgagaa
    12121 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
    12181 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
    12241 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
    12301 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
    12361 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
    12421 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
    12481 ggatttttta tattattttg tggatcatgc atgttttaca ggcattaagc ggcaaaaaga
    12541 tcatataacc tgttctggaa aacgcttgcg cagcaagaag attgcatgaa ataaaagcga
    12601 gtagggaaaa gtggacc
//
LOCUS       DAZGOB010000138        12579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGOB010000138 DAZGOB010000000
VERSION     DAZGOB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.138"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            245..1075
                     /gene="yjiC"
                     /locus_tag="ACHLIF_004154"
     CDS             245..1075
                     /gene="yjiC"
                     /locus_tag="ACHLIF_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO9608056.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVENYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1216..1989)
                     /gene="uxuR"
                     /locus_tag="ACHLIF_004155"
     CDS             complement(1216..1989)
                     /gene="uxuR"
                     /locus_tag="ACHLIF_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO9608057.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(2204..3664)
                     /gene="uxuB"
                     /locus_tag="ACHLIF_004156"
     CDS             complement(2204..3664)
                     /gene="uxuB"
                     /locus_tag="ACHLIF_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO9608058.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3745..4929)
                     /gene="uxuA"
                     /locus_tag="ACHLIF_004157"
     CDS             complement(3745..4929)
                     /gene="uxuA"
                     /locus_tag="ACHLIF_004157"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO9608059.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5381..5473
                     /locus_tag="ACHLIF_004158"
                     /pseudo
     CDS             5381..5473
                     /locus_tag="ACHLIF_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5716..6618)
                     /gene="fimH"
                     /locus_tag="ACHLIF_004159"
     CDS             complement(5716..6618)
                     /gene="fimH"
                     /locus_tag="ACHLIF_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO9608060.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGSVLSNFSGTVKYSGSS
                     DPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTVDAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6638..7141)
                     /gene="fimG"
                     /locus_tag="ACHLIF_004160"
     CDS             complement(6638..7141)
                     /gene="fimG"
                     /locus_tag="ACHLIF_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO9608061.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7154..7684)
                     /gene="fimF"
                     /locus_tag="ACHLIF_004161"
     CDS             complement(7154..7684)
                     /gene="fimF"
                     /locus_tag="ACHLIF_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO9608062.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7694..10330)
                     /gene="fimD"
                     /locus_tag="ACHLIF_004162"
     CDS             complement(7694..10330)
                     /gene="fimD"
                     /locus_tag="ACHLIF_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO9608063.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10397..11122)
                     /gene="fimC"
                     /locus_tag="ACHLIF_004163"
     CDS             complement(10397..11122)
                     /gene="fimC"
                     /locus_tag="ACHLIF_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO9608064.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11159..11698)
                     /gene="fimI"
                     /locus_tag="ACHLIF_004164"
     CDS             complement(11159..11698)
                     /gene="fimI"
                     /locus_tag="ACHLIF_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9608065.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11763..12311)
                     /gene="fimA"
                     /locus_tag="ACHLIF_004165"
     CDS             complement(11763..12311)
                     /gene="fimA"
                     /locus_tag="ACHLIF_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO9608066.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3139 a         3245 c         2964 g         3231 t
ORIGIN      
        1 agacatcaat atagcaacat caaattccaa ttagtaataa gatatgcttt taaattgcat
       61 gaaaataatt tcaaatgagc gttttttgat actttagagt gtttcctacc ctttaatatc
      121 accgtgtaga gttgatatca ctacaacaga aacatgcatg tcatttgttt acttctcatt
      181 ttactacaga aaaatttatc tatttttcta aacatcagtg aaatactcat aaaaggtaac
      241 tattatgtct atgccattaa gcaataccct tcagagccaa attattacag ataatcactt
      301 ccttcatcat ccaaaagtcg acagtgagtt aacacgtaaa tatgaacgtg cacgcctgga
      361 tacggaaaat atctatcttc tgcctcttgc tcgtggcaat aaccataatt acgatggaaa
      421 aagcgttgtc gaaattagaa aacttgatat tagtaaagag tcctggccat ttaattatgt
      481 gacagaagca tgcagagaat ccgatggtat tacaacaaca ggacgtatgc tctatcgaaa
      541 cttgaaaatc acctcagctt tagacgaaat atatggtggc atatgtaaaa aggcgcatgc
      601 tgctacagaa ttagcagaag gtttacggct aaatttattt atgaagagcc ctttcgatcc
      661 tgttgagaac tatacagttc atgaaataac attaggccct ggttgcaatg ttccaggtta
      721 cgccggaacc acgataggat atatttcaac ccttccagtt tctcaggcaa aaagatggac
      781 caatgagcag ccaagaattg atatctatat tgatcagata ataactgtct caggggtagc
      841 aaattcttcc ggattcgccc ttgccgcatt acttaatgct aatattgaga tgggaaatga
      901 cccaatcatt ggtatagaag cctatcctgg taccgcggag atccatgcaa aaatgggata
      961 taaagttatc cccggtgatg aagatgcgcc actcaaacgc atgactctgc aaccgtcatc
     1021 tcttccagaa ctgtttgaat taaaaaatgg tgaatggaat tatattggta aatagccact
     1081 gagaagaatg agtactaata ttcaggttat acagatattt aaacaatacc ctgcaattta
     1141 caaagcaata ctcattgcga cttcttaatt taagaaagcg caaggaacgt ttacccttgc
     1201 gcttattata ataagtcaga cgtcgacttt atccagcggc catgaatcaa acagatagcc
     1261 gtcaaaataa atatcgtcaa cgttcgagaa ttccagcaga cgttgcttaa cgttttccag
     1321 atgctgccac attgccagct tcgccgctcg ggcatctttt ttgattaacg cggcgaggat
     1381 ctgtttgtga tcgcccaacc actctttgcg atacaggctg tcatccagat ggctgtgcaa
     1441 ctggatccac attggattgt tttcccgcca ctgccaggac tgacggaata actccaccag
     1501 catgctgtta tgcgttgctt cggcaatagc gagatggaac tgcatgtcac cgctttcgct
     1561 gctgcccggc gcactggaag ccagttcacg ctcttccagt tgcaatgcct gacgcatttt
     1621 gacgatatct tcgcgggtag cctgcaaagc ggcaaactcg gcgatgttgc tctccaataa
     1681 ctgccgcgcc tgtaacagct caaaaggacc ggcatcgttg cagacgttgg catccggact
     1741 gtctgtgttc tggctgcctg agttatcaag aacatagata ccggcacccc ggcgtacttc
     1801 caccagccct ttgatctcca gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc
     1861 aagcatttct gcaatttcac gctccggcgg cagccgttcg ccaggattgt acggcgtctt
     1921 tatgatcaga tcgcggatca tcgccccgac ttcctggtaa ggtctttgcg cagaggtggc
     1981 agatttcatg gtgttgtgtc cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt
     2041 gaacgcctta tccggcctac aacgggcaat tttacggccc ccgttaatct caaactatac
     2101 cattagcact ggaagttggc acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa
     2161 tatcagtttg attgaccagt aagtctgtag ggtaatcagt tagttacagc gcagccacac
     2221 actcgcgcgc accgcgttcg cacagctgct gatatgccgc cgtcactgcg ccaacaaagt
     2281 cggcattctg cggcagatca tcggcaaaaa taccgctcag gcccagcagc gctttcacgc
     2341 ggtctgcgcc ctgatactgc gcgttgacct tctggaactc cgccagcatc gggtcgacca
     2401 cgtcaatggc attaccctgc tcatccacgc cctgggtgta acgcatccag ccagccacgc
     2461 ccagcgccag gtgacgccag ctgccgccgt tttgcaggtg cagacgcacc gggtccagca
     2521 gacgctgcgg taacttctgg ctgccgtcca tcgcaatctg ccaggtacgg tgacgcagag
     2581 acgggttgct gaaacgctcg atcagcagcg tcgcataggc gttcaggtcc gtaccttccg
     2641 gcatcgacag cgttggcgct tgttcctgca tcatcaagga gaaggccgct ttgcgataat
     2701 ccgggttagt catggtgtcg gcaatggttt catagccgcc gaggtaaccg aggtatgcca
     2761 ggaaagagtg gctgccgttc agcatacgca gcttcatcat ttcgaacggc acaacgtctg
     2821 caacgaactg tgcgcccact ttatcccagt ccgggcgacc gttaacgaag ttatcttcaa
     2881 tcacccactg acggaacggt tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt
     2941 cagcaatttc ctgcaaggtt tctggcgtcg ccgccggaac gatgcggtca accatggtgc
     3001 acgggaaggt gacgttttct tcaatccatg ccgccagctg cgggtcacgc gcctgagcca
     3061 gtcccagtac cgcgaccttc gccacatggc cgttttcacg cacgttatcg caggacatca
     3121 ccgtaaacgc tttcaaccct ttttcacgac gcagccgcag ggcttcgacg atgtaaccaa
     3181 tcgcggactt cggcgcagtc gggttttcca gatcgtgctt gatcagtggg ttattgagat
     3241 ccagctgacc gcttgccgca tcagcgcagt agcctttttc cgtgactgtc agagagacaa
     3301 tcgccgtctg cggacgcgcc atcgcattga gaataccttc gcagccgtcg atttccggat
     3361 gcagcgcttc tttcatcgaa ccgataattt tcagctcggt gctttccgcg cctttttccg
     3421 ccacggtgta cagcagttgc tgttttttca ggttttcgat cagcacgcgg tcgttgcctg
     3481 gcatcaggtt aacttcgcag ataccccagt cgctgtcggt gctttccagc aggtgatggg
     3541 tatacagcgc ctggtgcgcg cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt
     3601 ccagacgaga atgctcccat gacgggcggg caaccggcag attgctgtca acaatagtag
     3661 tcatcgtaaa ctccagaatc cgtcttcaga gagtgacggc aaggaagaag gaaccggcac
     3721 ggcgcgcagc catgccggtg ggtattaacg gctaaagaaa gcgcgctgga tcgccagttc
     3781 gacaccgcga acttcggcca ggcctttcag acgaccaatt gcggagtaac ctgggttggt
     3841 tttcttcttc aggtcgtcca gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc
     3901 ttctttgcct tccgctttac gacggtgttc ttcttcaaca atcgctttca ccacttcgta
     3961 catatcaacg tcaccgttca ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg
     4021 catggtggag cgcagatggg tgaagtaaat acgcggaccg aactgcttga tcatatcaac
     4081 cagatcgttg tcagcacgca cgccgtagga accggtgcac atggtgaaac cgttcgccat
     4141 gctgtttacg gtatcaacca tccactgcat atcttcaatg gtggaaacaa tgcgcggcag
     4201 gccaaggatc gggcgcggcg gatcgtccgg gtgaaccgcc atacgcacgc caacttcttc
     4261 agcaactgga ataatcgctt tcaggaagac ggcaaagttt tcgcgcagct tcgctttatc
     4321 gatatctttg tacagctcca ggtgcttacg gaactggtcg agggtatacc cttcttccgc
     4381 gcccggcaga ccggcaatga tgttacgcgt cagacgcgct ttgtcttcgt cgctcatagt
     4441 ggcgaagcga acagcagcct gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg
     4501 acgtttcagg atatgcattt cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt
     4561 ggagccgtct ggcagcacgt attcgaggtc agtacgggtc cagtcgagca ccggcatgaa
     4621 gttgtagcac acggtgcgaa tgccgcactg cgccaggttg cgcagagtct gctgatagtt
     4681 agcgatccac tgctcatagt tgccagtgtg ggttttgata tcttcgtgaa ttggtacgct
     4741 ttctacgaca gaccacacca ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc
     4801 ttctacggac catacttcgc cgttcgggat atggtgcagc gcggtaacca cgccagttgc
     4861 gcccgcctga cggacatcag ctaaagaaac cggatcgttt gggccgtacc agcgccaggt
     4921 ctgttccatg atttttcctc tttcacatag tgggttccac agcaaagaga cgcggaaccc
     4981 tggagttgtt taggtatccg gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg
     5041 agatggcgta taagttctac cttttggtaa gacatgtcaa cccgaattct gaaattggtt
     5101 aaccacatca caagaatttc actctcatgg ttgttgctgc aaattgcagc aaagggtaat
     5161 cgatggttat tgatcagagg atgtgacatt catcgcaaca atggttgacc aatttacata
     5221 acatgtcgcc caaaataaca acggttcaac caggtaaggt aaaacgttat gcatgtgctt
     5281 aacattctct gggtggtatt cggcattggc ctgatgctgg tactgacaaa gaccgcaggt
     5341 tcagacacat tcgctgcatt agcaatgcct cttagataat tttgacctgt cagtaaaaga
     5401 cacgttgaaa acctggggtc tgctggagct ggtcaactcc gtggttgggc tgattattag
     5461 caaggtagcg taaaagaaca ccagagcctg ccaatggcag gctctgactg atgacaaagt
     5521 ctaaaaatgt gcccggacag tcccctcgcc cctccgggga gagggttagg gtgaggggaa
     5581 cagaccagca aagtgcgctt tgtttatgcc agatgcgacg ctgacgcgtc ttatctggcc
     5641 tacaaagggc taacgtgcag gtttttagct tcaggtaata ttgcgtaact gcattagcaa
     5701 tgccctgtga tttctttatt gataaacaaa agtcacgcca ataatcgatt gcacattccc
     5761 tgcagtcacc tgccctccgg tacgtgcgta atttgccgtt aatcccagac ttaccgccga
     5821 agtccctact gctcctaacg ataccgtgtt attcgctgga ataatcgtac cgttgcgcgt
     5881 caactgtacg ccgacgccct gcgctggtga aaacgacgcg gtattggtga aaatcgagtt
     5941 gcccgcatct acggttgtgc cggagaggta ataccccaga ttttggcttt tcgcacaata
     6001 aacggtaaga ggaattggca ctgaaccagg gtagtccggc agagtaacgg tgacatcacg
     6061 agcagaaaca tcgcagccgc cagtaggcac caccacatca ttattggcgt aaatattcca
     6121 cacaaactga aaatcatcgc tgttatagtt gttggtctgt cgcaaaataa gcacggcaat
     6181 taatgagcca gctttaatcg ccaccccgcc cgcactgctc acaggcgtca aataaagcgc
     6241 caccggccac ggcttatccg ttctcgaatt ataaacaacg cgcggcgttt cgctggtggt
     6301 aggaaatgga tcgctactgc cactatattt tacggtcccg gaaaaattag ataacacgct
     6361 gccataagcc gagcctcgtt gcagtgtgac atagtctgta atggtttccg gataatcgtt
     6421 atggcaaaag atttgcgtcg aaagatccac gaccaggttt tgccccacat tcacggcggg
     6481 cgcaaggttt acataaacat tggcgctgcc accgccaata gggatagcgg taccattggc
     6541 ggttttacag gcgaatgacc aggcatttac cgaccagccc atcagcagta cagcaaacag
     6601 ggtaataact cgtttcatta caatcatctc ttcgggttca gctgtaggta taggtgatgc
     6661 taatcactgc ctgaatggtt ccctgagtgg ctccgccatt taccgtcaat gctctgacct
     6721 gtaacgggaa gtgcgctgat tgtgaggaat catccacctg aactgttttg gttgcgccag
     6781 tattcaatgt gttgccactg tcatcctgta gctctaactg gatgttttgc gcggtcccct
     6841 ggtttttata atatccggta ctgtcggctg ccccgctgaa gctggcggtg accctcgacg
     6901 ttcccaccgg acaattagtc aactcaagcg caacatcatg ccaggccgat gccgccccgg
     6961 cagacatcag actgaaagaa taaagatcgc cgagatcaac cgtggcattg gtggtggaaa
     7021 ccgtacacgg tttggcgacg accttaccgt tcaccgtgat ggtgacatcg gctgcctgta
     7081 tcgtcgcact tgcgagcgcc aatattgccg ccaataaata cccacgtttg cgccatttca
     7141 tgagcatctc cagttactga tattcaagag tgaaggtagc cgtggcattg atatgccccg
     7201 cagtgacagg cacctgtgtc gccattagcc gggcgtaaaa attcaacgta tttggtttac
     7261 ccggcgtcag ggtcgtccac gaaatcgcgg acgatggagc attaaggggt atttgatttt
     7321 gctgctcatt cagaagctgt attcccagtc ccgaagccgc tgacaccgta ttttcaagtg
     7381 caagcagatt ggcattgtgg ctatctgcaa cgccggtaaa cccaactttt acggcagaaa
     7441 cggcattacc acagggtgac agcaaaatac gaaatggaac aacaggagtc gtcgcgccaa
     7501 tgttgttaaa ttgcttcgcc gcgttttcca tcagatcaac agtaaaattg gttgattcag
     7561 cggccacact acagccgtta tctctgacat agccgcggat agtaatcgtg ctatccgcag
     7621 ccaaagcgtg actcaccgcc agccacaaaa aagcgcacag aagataaaaa ggtttgtttc
     7681 tcatcacgcc cccttaacga cattcagctg atagctgggt taataactgc tgctgactct
     7741 ctggtggcag ttgataattg gcgacacagt gagcattttc ctcttctccc catttcacct
     7801 gaacttttcc cgctaaaggc attccgctga ggtaaacctg accattatcc gcaacaatgc
     7861 cgctactctg gctactctct gatgtcacca tcgccccaaa cggcagcggc ttattattgt
     7921 gggttagcgt catgagcagt tttatcccaa cgcgcgcttt aaactctgct cgcacgatcg
     7981 ccccacgagt gggaacaacg ttagcgaccg cgttatctaa atcgacgtta tcagccaggg
     8041 tattggtatc cagcgccact ctgttttccc tatattcagt ggcataaggc agcacggcat
     8101 aaccgcgcca gtcggtacgc acccccgtct ggttttcgac ttttgcatct tttgcgccag
     8161 gcgctttaac aagcaccacc gtatcgttta acggctgccc cagcgttacg ccattggcat
     8221 gagccaatac cccaccgctg actccgtaat agagctgctt aatatcatcg ctatggctgt
     8281 aaccgatatt ggcattgccg taaccaccgc gataattcag cgtggcgtag cctgtgcttc
     8341 cgctattacc atcgcctccc ccggcatagc cggtttgcac gctatagctg aggttgttgt
     8401 cttccagcaa cgtaccgtat acaccagcca gattggtcat ccgaccgttg agatcgtgtg
     8461 acatgctgta gctggcactg gcatgtcgcc actgagattt actgtcagaa cgcagccagt
     8521 ggctgaaagg aatattgacg ttaagcgcta acatctgatc acgtcctttt tgccaggcgt
     8581 ttttcgtcag gctatagctg agcgtccagt tgatatcttc gaacgcagta tttaatccag
     8641 cctggaattg ctcatcgaca ttactcgttc cccaataagt ttgatggcta ccactcaaat
     8701 acagtgttga tgtgcgcccg agttgctgag taacggtgag ctgtaatttc ccgcgtttgt
     8761 tataagcgag gttgtaatag tcggtgaatt tcggcttaac ctgaataact ccatcctgtg
     8821 tttcgatgtt gtagccattc attcgactgt atgttgtatc agcgaaatta aaatatccgc
     8881 tggtcgaata acggtaaccc actaactgaa tattcgtgcc tgattcattg agcgatttgt
     8941 tatagagaaa acgcaccgat tgtccgtcat gctgactgtc atcgggaagt gtggaattag
     9001 cctgcgtcat atccacagac agagcgccca gtgcccccat gtttttcccg ataccgaaat
     9061 taaaagcacg ataacgatcc gccagttgcg ttccaccata tattgtccag ccagccggaa
     9121 ggccgtggag taatgtactc tggaaaaagc ggggtttttc ctgctgcgca tttccactac
     9181 ggtattctcc tgccgtaatg gaataacgag tatgcccttc acgttgcaaa agcgggactg
     9241 acgaataggg tacggtaaaa atctgcgtgc tgccgtcagc ctctttgatc gttacctgca
     9301 agtcaccact attacctgcg gcatagatat cgttgatggt aaaaggcccc ggtggcaccg
     9361 tactattata aatgtcatac ccattttgtt taatagtgac ctgtgcagta ccacgagcaa
     9421 taccgtggat caccggggca aatcctcttt ggctatcggg taacatattg tcatctgagg
     9481 ccaattgtgc gccgcgaaag ttaataccat cgaaaatatc gccctgagta taaccatcac
     9541 ccagcgtcag ccgggaacgt aacggtatta tgtctcgctc aagccaggta ttgatatgct
     9601 gccatttatt tttgctacct gatgatctgt cgctactgtt ataactccag gtggtattgt
     9661 cgcgtaaacg ccacgcacca atatttaacc cactctgtag gtttaaatat gcataatggc
     9721 tgttaccccc aatccgattc tgtacactat ttccgctgaa attataattg agcaatccgg
     9781 cattaatacc gggatcccat aactcaggag gaatataacc acgcgcgcga ttactcataa
     9841 atgcctgagg gatcgtcagg ttcagtcgct gctgaccaac atccagatgc gcagtagcgt
     9901 cctggaccat tgtggttaat ggcacacagg catcatccgc cagcagattc ataccggcga
     9961 cagaagccgt attcagcccc atactggcga gttgcgcgcg tgtcaggcag ggaacaatcc
    10021 cttgttcact gtcgcccgta ttaaatgtga catcacgcgt tgccatataa ccattattca
    10081 aatagatatc gacgcgatac gtccctggcg gtaattcttg cccattttca aaacgcgata
    10141 aatcggccac agcctgggga tcatccgcta aaaaacgcgg gttaaaatag agttcggcag
    10201 atgacaaagg tgcctgtgcg gcaaaagcac aggcaacaaa gagccggaca aaaaaaccag
    10261 ccaaacgatg cttacgaata tgcaagcatt gtgtgtttcg ctggtaaagt cttaaattca
    10321 gatatgacat tattcctccg gtttaaagtc acccggcagt caattctttt tattcaggcg
    10381 aaaaattccc cctgcgttat tccattacgc ccgtcatttt gggggtaagt gcaccataat
    10441 catttattgt tcggtaagta atattgcttc ccgcatcaga aggcaattta accgtgcttt
    10501 cgcccattgg aggcaccaag gcattttcaa gaacccgggt tccggcattc aactctgtta
    10561 ccgtcaggta atagggtgtc gggttaatca gcgtcagaga attcgcgcta cgacgaaatc
    10621 ttaatttttc tgcggcctga tcgggtggca acgctaattt agccgggcga tagtacagtt
    10681 taatgcggct gataattgcg agctgtagcg tattctcagt caattttgat ttatccattg
    10741 acggaatcgc tttaacattc atccagaata aactttcccg atcctgtggc aattggttat
    10801 ttgttgcatc aagaatacgc aaggtattct ctttttttcc cttcatcgca aacagaggag
    10861 gcgtcacgat aaaacgacca tcctttacac catcggcatt ttccacccat gattgaatta
    10921 aataggtact attttcatca ttatttgtca cggcaagttg cacttgtttt tgccctgccg
    10981 gataaattac gcgagtcgca cctaaggcca ctcccgcttc agcgcgtccg gcaaccatca
    11041 ttgccataaa catcaggata cctgccagca agcagaatgt tatttcctgc gatttcctta
    11101 cattgacgtt tttattactc actgtcctgt tcctgttatc acattatctg ctgaacaatt
    11161 actgataggt taaagagaac caggcctggg cattggcgat gccgccagta acccgacgcc
    11221 cggtagcacg atatttggcg atgaaatgta gcgaagtaga gcctgagtaa agccgtttcc
    11281 agtttgctgg aggacgatta atcggtacga ggtttccttc atcatcaaac aacgctacgc
    11341 caatattggt ggctatccct ggcccctctc ccacggaaag cacatccgga tttttaccat
    11401 ccgcgacacc gtgaaacgcc acccccacac gttcactcac caccgtgcta cattcccgta
    11461 aatgaataac aaaaggcacc ggtgcgctat cttcccctgc cgcatgaaac cggttactgc
    11521 tgatttgccc catattgacc gtcatttgtt tatcaccggc ttcaatccgg caagtttccg
    11581 caataatgac gccctgaaat tgcatatttc cgccgggcag cgtggtattc catttatttc
    11641 cggccagagc aaacattggc agcaacgacg ctaacagaaa taatctcttc cttttcatcg
    11701 ttatttttat cgcacaaggg tgggcatccc tgcccgtaat gacgtccttg aacctgggta
    11761 ggttattgat actgaacctt gaaggttgca tccgcattag ctgcacccgg ggttgcctcg
    11821 ccgattgcat aataacgcgc ctggaacgga atggtgttgg taccgttatt cagggttgtt
    11881 tgtgcactaa atgtcgcacc gtccagcgtc agagcattgc ctgttctgtc caggatctgc
    11941 acgccaacgt ttgttgcact acctgcagca gaactctgca gagccagtac atcagtacgc
    12001 gtagcatcaa ttgccgtacc taagaaggca acagcggctt ttgtggcaac agtggtatcg
    12061 caatcattca actgaatgtt aaaaccaacg gcagagctgg ttgctccagc ctgcttcagg
    12121 ctagcggtac gaacctgtcc caactgaacg gtttgatcaa cagagcctgc atcaactgcg
    12181 caagcggcgt taacaacttc ccctttaaag tgaacggtcc caccatttac cgtcgtagta
    12241 tcggccagag ccgctgcgga actgagggac agagccgaca gaacaacgat tgccagagtt
    12301 ttaattttca tgctgctttc ctttaaaaaa actatttcta aatcgacatg ggcagtcgtt
    12361 ctgtacactt tgttttgtca acgagtttga ctgccaacac tgcacagttt tcccccaaaa
    12421 gatgaaacat ttggggccat tttgactcat agaggaaagc atcgcgcaca aactttttca
    12481 gtttatttgt tggcttaata ttctattgtt atctttattt atagattttt atattgcatg
    12541 aggtggtttt tggagagaag aatgaggaag atgcgtcga
//
LOCUS       DAZGOB010000139        12490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGOB010000139 DAZGOB010000000
VERSION     DAZGOB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.139"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(178..1602)
                     /gene="patD"
                     /locus_tag="ACHLIF_004166"
     CDS             complement(178..1602)
                     /gene="patD"
                     /locus_tag="ACHLIF_004166"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO9608067.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1624..2418)
                     /gene="ydcV"
                     /locus_tag="ACHLIF_004167"
     CDS             complement(1624..2418)
                     /gene="ydcV"
                     /locus_tag="ACHLIF_004167"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9608068.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2408..3349)
                     /gene="ydcU"
                     /locus_tag="ACHLIF_004168"
     CDS             complement(2408..3349)
                     /gene="ydcU"
                     /locus_tag="ACHLIF_004168"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9608069.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPRLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(3350..4363)
                     /gene="ydcT"
                     /locus_tag="ACHLIF_004169"
     CDS             complement(3350..4363)
                     /gene="ydcT"
                     /locus_tag="ACHLIF_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9608070.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4381..5526)
                     /gene="ydcS"
                     /locus_tag="ACHLIF_004170"
     CDS             complement(4381..5526)
                     /gene="ydcS"
                     /locus_tag="ACHLIF_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO9608071.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5771..7176)
                     /gene="ydcR"
                     /locus_tag="ACHLIF_004171"
                     /pseudo
     CDS             complement(5771..7176)
                     /gene="ydcR"
                     /locus_tag="ACHLIF_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(7255..7671)
                     /gene="hicB"
                     /locus_tag="ACHLIF_004172"
     CDS             complement(7255..7671)
                     /gene="hicB"
                     /locus_tag="ACHLIF_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO9608072.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(7717..7893)
                     /gene="hicA"
                     /locus_tag="ACHLIF_004173"
     CDS             complement(7717..7893)
                     /gene="hicA"
                     /locus_tag="ACHLIF_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO9608073.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            8115..8345
                     /gene="yncJ"
                     /locus_tag="ACHLIF_004174"
     CDS             8115..8345
                     /gene="yncJ"
                     /locus_tag="ACHLIF_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO9608074.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(8437..10398)
                     /gene="rlhA"
                     /locus_tag="ACHLIF_004175"
     CDS             complement(8437..10398)
                     /gene="rlhA"
                     /locus_tag="ACHLIF_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO9608075.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFFANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(10471..11007)
                     /gene="sutR"
                     /locus_tag="ACHLIF_004176"
     CDS             complement(10471..11007)
                     /gene="sutR"
                     /locus_tag="ACHLIF_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO9608076.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            11060..12274
                     /gene="ydcO"
                     /locus_tag="ACHLIF_004177"
     CDS             11060..12274
                     /gene="ydcO"
                     /locus_tag="ACHLIF_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO9608077.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(12314..>12490)
                     /locus_tag="ACHLIF_004178"
     CDS             complement(12314..>12490)
                     /locus_tag="ACHLIF_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9608078.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     PLKQEPTEMIQATA"
BASE COUNT         3059 a         3353 c         3058 g         3020 t
ORIGIN      
        1 aataacgata ctctaaagcc ttatgctaat caactgtgac aggatgaaaa atcgccatca
       61 aacatttagc ctaattttta cgctagttat taatgtaatc attctaatac ttaaccgcca
      121 ttaaactcag aacctaaaac gatagtggcc gtcctataac agccactaac atctaactta
      181 atgtttaacc atgacgtggc ggacgacggt gtaatcctcc agcccataaa gtgacatatc
      241 cttgccgtaa ccagaaagtt tctgcccacc gtgcggcatt tcacttacca gcatgaaatg
      301 ggtattgacc caggtacaac catattgcag ccgtgcgctg acgcgatgcg ccctgcccac
      361 atctttcgtc cataccgaag atgcaagtcc gtactggctg tcattcgccc agttcaccac
      421 ctgttcttcg ttgtcgaagg gcgtaacact cactactgga ccaaatacct ctttttgcac
      481 gatggcatcg tcctgtaatg cgccagccag cagcgtcggc gcatagtaat agccattacc
      541 cttgcgcttt tcaccgccag tgatcacttt gatgtgccct gtcgctttcg cctcttctac
      601 tgccttgctg acgcgttcga gatgcgccag cgagcttaaa ggtccaagct ccgtagactc
      661 gtcatccggc gcaccagatt ttaacgttgc caccgcagca cccagttttt ccaccagcgt
      721 atcgtaaatg cctttttgcg cgtagatccg acaagccgca gtacaatcct gtccagcatt
      781 gtaatagcca aatgtacgta caccttcgac cactgcttca atatccgcat catcaaaaac
      841 aatcactggc gctttgccac caagttccat atgagtacgc ttaatggacg acgcggtatg
      901 gctgatgatg tgctcgccgg tggcgataga gcccgtcagc gacaccatcc gcactttggg
      961 atgaccggtc agcggatcac ccaccgtttt gcctctgcca aacagtacgt taatcacgcc
     1021 tgccgggaag atatctttcg ccagctctgc caacttcaac gcggtcagcg gggtaatttc
     1081 tgatggttta agcactacgc agttccctgc cgccagcgcc ggagccagtt tccacgctgc
     1141 catcatcagc ggataattcc acggtgcgat agaagccacg acccccaacg gatcgcgacg
     1201 gatcatcgaa gtatgacctt caagatattc acctgccgcc agaccattca gacagcgcgc
     1261 cgcacccgcg aaaaagcgaa aaacatcgac aatcgccggg atttcatcat tgaacgcact
     1321 atgcagcggt ttgccacaat tacgggactc cagttcggca aaaacctgac cattttcttc
     1381 gataacatca gccagtttca gcagacattc cgcacgcact ttcggcgtgg tttgccccca
     1441 ttcggcaaat gctgcatctg ccgcgcgcac agcagcatcg acctgctctg cggatgcctc
     1501 ggcaatctcc agtaaaacgt cccccgttgc cggattatag acaggctgtt tttccccttc
     1561 gccgctaacc agttctccgt taatcagtaa cttatgttgc atagcatatt cctgtatcag
     1621 tggttattga ccattgtcgc cttcgcgggt tagccaccag gcccccagga tcggcaaggt
     1681 tgttaccaac ataaccagca gtgccaccac gttagttacc ggtacatcac gcggtcgccc
     1741 aagctgattg agcaaccaca acggtaacgt tcgttcatga cctgccgtaa aagtcgtaac
     1801 gatgatttca tcgaacgaca aggcaaacgc cagcattcct cctgccagta gcgccgaact
     1861 gagattcggc aacactacgt agcggaaggt ttgccagcca ttggccccaa gatccattga
     1921 cgcctcaacc agactccacg aggtgcggcg aaaacgggcg atgacattgt taaacaccac
     1981 cactacgcaa aaagtcgcat gaccgaccac gatggtgaaa aatcccggct ccagattgat
     2041 ggttttaaaa gcggttaata gcgccagacc agtgacaatg cccggcagcg caatgggcag
     2101 cagcagtaac agcgaaatgg cgtttttgcc gaaaaagtct cgtcgccaca gcgcggcagc
     2161 tgccagcgtc cctaacacca gcgccattaa tgtcgccagc gccgccactt taagtgacag
     2221 tgtcacggca tcaagaatat cactacgctg tgctgccacg ctaaaccagc gcagcgtcag
     2281 gccctgcggt ggaaaactaa acgccgcatc ttcagtgtta aacgcataag cggcgataat
     2341 caggatggga aaatgtagga aaaccacgcc gccccaggcc gccagtttga ggaaaaacgg
     2401 tgcgcgttca gagtgcatcg aacgctccca gacgtttcac gaacgccagg tacagtgcga
     2461 tgagaacaat cggcaccagg gtgaatgccg ccgccatcgg catattgcca atcgccccct
     2521 gctgggaata aaccatattg ccgataaaat atcctggagg accaaccagc tgcgggacga
     2581 taaaatcgcc cagtgtgagt gagaaggtaa agatagagcc agcggcaata cccgggattg
     2641 ccagcggcag caccacatag cgaaaggttt gtcgtggacg tgcgccgaga tcagccgacg
     2701 cctgcaacaa tgacggcggc aaacgctcaa gcgccgcctg aacgggcagg atcatgaacg
     2761 gcaaccagat atagagaaac accagaaagc gccccagccc ggaagttgac agcgtatttc
     2821 cgcccaccgc aggtaatgta aggaacgcag tcagcagtgg ttccagccca agatgttgta
     2881 aaaaccactg agccacgcca tcttttgcca gcaataacgt ccaggcatag gctttaacaa
     2941 tgtagctcgc ccacatcggc aacattaccg caatataaaa aaaagctttc attttcccgc
     3001 tggtatagcg cgccatatac cacgccattg gaaaagccag aatggcgctg gcgatagtga
     3061 ctgccacagc catagtcagc gtgcggagaa tgatgtcgta attcgccgga ttaaacagcg
     3121 cacggatatt cgccagcgtc agttccggcg ttaccgacat ggtgaaatcg tcaaaagtat
     3181 aaaatccctg ccataacagt gtcagcagcg agccgaaata gacaatgcca aaccacatta
     3241 gcgggccaag cagcagtaaa aacagcccca gcctcggatt acgccagaag aaaccggaca
     3301 ccttacccag acctggacgt gaaggtgatt gcaatacatt catcgccatt cacctctcct
     3361 caaccagcgg caccatcaca tcacgcgacc aggaaaccat cacctgttgt ccgggcgtga
     3421 gcgtggcagg cagttcttcg cctgtcatat tggcctgact cacaagcagt ttttcaccgc
     3481 cgttcaattt cagttcaaaa cgagttgccg cgccctgata ttgcaccgcc tggatcgtgc
     3541 cattggcctg cagttcacca ggggtgttga ggcggatatg ttccggtcgc agggcgaagc
     3601 ttcccgtcat gccacaaagt ttctctgcca tcagtccatc aaaaacgttg gatgtcccga
     3661 cgaatccggc aacaaacggc gtgcgcgggc gcatatagag atcgcgcggg gaatcgacct
     3721 gctcaatgcg tccattattg aaaaccgcca cacgatcgga catcgataac gcttcgccct
     3781 gatcgtgggt aacgaagata aaagtgatac cgagagactg ttgcagtttt ttcagttcca
     3841 gctgcatctg ctcacgcaat ttgagatcca gtgcgccgag cggttcatcc aacagcaata
     3901 cgcgcggttc attcaccaat gctctggcga tagcaacccg ctggcgctga ccaccagaaa
     3961 gttgtgacgg tttacgttga tgtacaaacc ccaacgccac tttctccagc gcctcttgcg
     4021 ccattgcgtg ccgctgcttt ttattcacgc ctttgaccat cagcccatag gcgacattgt
     4081 caagaatcga catatgcgga aatagcgcgt agtcctgaaa gacagtattc acgtcccgct
     4141 cccacggtgg cagattgctg gcgggtttac caaagataga gatagccccg ccggaaagct
     4201 gttcgaagcc agcaatcagg cgcaggcagg tggttttgcc ggagccggac ggccccagca
     4261 tagagaagaa ctcaccatct tttatcgcaa tactgacgcc atctactgcg cgcacgtcac
     4321 cgtacaaccg cgagacgttg tcaaactcca ctgcgtacgt cataaagcac cccagcgaag
     4381 ttagcgaccg cccataatgg caatgtaatc ctgcgtccag cgactgtagg gaacaaactt
     4441 gcccccttct gctataggcg ttttccagaa ggcgattttg tcgaaatagt taaaaccgtt
     4501 ggtttcacaa cctttttcgc ctaataacgg actggcttta cacccttccg gcactaccgg
     4561 taacgagcca aaccaggccg ccacatcgcc ctgcactttt ggcgttaatg accagttcat
     4621 ccatttgtag gcgcaaaccg gatgtttcgc ttcgctatgc agcatggtgg tatcagccca
     4681 accggtaaca ccctccttcg ggaaaacggt agcaacaggc tggccttcgg ctttcagggc
     4741 gttggcctga tagggccagg cactggaagc aaccacaccc tcgtttttga aatcgctcat
     4801 ttgcacggta gtgtcatgcc agtagcgatg gatcaaactg tgttgagcgc gcagcacttt
     4861 cagcaccgcc tggtactgtt cttcggttag ttgatacgga tcgctgatgc ccaactgcgg
     4921 ctgagtggct ttaacgaaca acgcagcgtc cgcaatgtag atagggccat cataagcctg
     4981 aacgcggcct ttattgctct tgccgtccgg cagattttgc tcaacaaaaa ccacttgcca
     5041 gctatccggc ggcgtcggga aggttttagt gttgtacatc agcaggttcg gcccccattg
     5101 gtaaggtgtg ccgtaaactt tgccgccaac attaaaccag tcgcctttaa ccacgcgcgg
     5161 atcgagcgtt ttccagttgg gaatcaaggc ggtattaatc ggctgcacgc gtttgcccat
     5221 aatcaaacgc agcgaggcat cgccggatgc cgtaaccaga tcgtaacccc ctttggtcat
     5281 cagactgacc atttcatcgg aagtcgcggc ggttttcaca ttcaccgcgc agcctgtctc
     5341 tttttcaaat tgcgttaccc agtcgtattg tttatcagtt tgtccgcgtt cgatgtatcc
     5401 cggccaggcg ataatatcca gtcgcccttc cggtttatct aaattggtag gtggttcggc
     5461 ggcgtgagcg gtcattattg tcatggtgag cgcacacagg ctgctgcggg caaatgtctt
     5521 gctcataagg tcttactcct gtcgtaatta attgttcagc cgccgtgccg taaaaatatc
     5581 tccttgttta accatagacg gcggcttgca ggcacacctt cgcccctgag aaaatttaat
     5641 caggttgtga gctaccgcgc attatgccgt ctgtaaggtt atcttctgga gtatagacaa
     5701 ggggttagga aatatgactg ctattcgaaa ttcatattac ccaatctaag aggaattaaa
     5761 ttaaacgcta ttacagccgt tcttgaataa gtttgcctaa ttgttttacc gcctgttctt
     5821 cacgttctcc ccactgccac gcggtattaa aacgaaaaaa acgtgaccag ttttcaccgg
     5881 tagaaaacat tttacccggc gcaatactga tatgatgcgt cagtgccgcc aggcttaatt
     5941 cgccggcatc taacggctcg gggagctcca gccagagaaa ataaccgctg tcactatgat
     6001 gaattttaac ttccgcaggc agataacgca gcagtgcctg ccaggcacgt tgtttacgtt
     6061 ccgcaagctg gcgacgcagg cgacgaagat gggcgtcgta tcggcgcgtg gaaaggtaat
     6121 ccaccagcgc aagctgcatc ggtgagctgg tggaaagcgt actcatcaac tgcaagcgtt
     6181 gaattttacg tgcatgtttt ccggcggcga cccaaccaat acgaaaacca ggcaccagac
     6241 atttcgaaaa cgaagagcaa tgcaaaacgc catcgtggcg atcccacgct ttcgcaggca
     6301 gcggtttttc ccgtccaaaa taaagttcgc tgtaaacgtc atcttcaatc agcgttacgt
     6361 tgtactgatt gagtaacgcc accagttgtg ctttttttgc ggcgttaagg taaatccgag
     6421 tggattctgg ctattagtca tcagccagca cgctttcacc ggatactcct gcaacgccag
     6481 ttccaacgcc tgaagatcta tcccttcttt aacatccgtc gccaccgata acgctttcag
     6541 ccgtagccgt tccagcgcct gcaacgcgcc atagaaacaa ggattctcta ctatcaccca
     6601 atcgcccggt tcagttaccg cttgcaaact gaggtttaat gcctctaacg ccccggcagt
     6661 aatgacaatt tcatcaggag aaatggtgat gccctgtaag gcatagcgac gagcaatagc
     6721 ctgacgcagt tctgcgtttc ctggcggtaa gttttcaatc acgctcatcg ccgtggcggt
     6781 tttgcttacc tgcgccagcg agcggtttag ttgttggagg gggaaaagtc gcgggtcggg
     6841 aaaggccgag gcaaacggaa cgaccgacgg atcgcgactg gcctgcaaca tatcaaaaat
     6901 ataagtgttg atatcgactg cttcatctcg agtgactgga atgactggcg ctttcggcat
     6961 ttttattgcc tgtggcgcaa cgtaataacc cgactgcggt cgtgcgataa tatatccctg
     7021 actttcgagc aactgatagg catggctgac agtcataaag ctcatgcctg aaagcgccac
     7081 ctggtcacgc aacgaaggca aacgatcgcc gggttgccag atacccgacg caatctgctc
     7141 gcgtaattgt tctgcaagct gctggtattt tttcattatc gaatcgtaat tatgtgcaga
     7201 tgattcggca gtctatatca gttgttaaaa atgacaactt ttcgttaact gtaattaaac
     7261 catcaccagc gataactctt tgccaagcgc ctttgccgca agctggacgg cgtcgatttt
     7321 tgtcgcatgg tgcaagttaa ataggcgagt aatctcctgt ttaggtttgc caattcgcct
     7381 ggctaactct tgctgagtga tttctgactg taaaaaagca tttaacagca atactttaga
     7441 ggcgacgctc aaaggtactt caataaagtg atcatgacta tttaatggcg aaggtaacgg
     7501 gataagctcg ttatcttcaa aataaaaatc aaatgcggtc agtaaagcat cttttgccgc
     7561 ttccatcgct tcagcgacag tttcgccctg cgtcaacgct tcagggatat ccacaaaaga
     7621 aaccatataa ccgccttccg gcgcgggtgt aagagtgacg ggataacgca taatttcgac
     7681 tgtccctcgc atgtcattaa cctttttaat tggcgattaa ctcaaaccga gttgtttcag
     7741 gattgcttta cgcaatggtt ctttaatctc atcgcagggg tgacgcggca tgacactgcg
     7801 cctcccatga aacctgagtt tcaaatggtt gctgccattc gctacatcga cgccctgaga
     7861 ttcgagccaa cgtctgaact cgctttgttt cacatcgcct ccctgccgct gagtatatca
     7921 acaaagtaaa cattagtgtt tacctccgtg tgtaatttgc tcgaaaatag cactaaatcg
     7981 ccgcaacggc agaatgccat gcaaaatcag gaagaaagac tccagaattt ctttgcgcta
     8041 atttgcaaaa ctgaataata acctcaagaa acgatcggca tcatagcggc aaaaggtaag
     8101 aaaaaggtgg caggatgttt acgaaggcgt tatcggttgt cttattaacg tgtgcgctgt
     8161 tctcaggaca actcatggca gggcacaaag gacatgaatt tgtgtgggta aagaatgtgg
     8221 atcatcagct gcgtcatgaa gcggacagcg atgaattgcg tgctgtggcg gaagagtcgg
     8281 cggaaggttt gcgcgagcat ttttactggc aaaaatcgcg caaaccagaa gcgggacaac
     8341 gttgagtcat ttgttgccgg atgcggcgag acgccttacc cggcaggcaa tatcatgcag
     8401 attcaatgag ttgtgagtaa ccagaaaact ggcgttttac ccttttcgct taggcaatgt
     8461 tttcagcagc tcatccggac ttacggatgc cactacgctt cccggtaacg gcattttcag
     8521 tatgtgattt ttgattttgc caatgacgtg catctcgcat gggcggcaat caaactttag
     8581 cgttaatact tcatcgccgt taaccagttg cattggcgtc gccttccagc ttttgatatt
     8641 gcctttcgcc tgtttcgggc acagattaaa ggcaaagcgc agacaatgct tggtgatcat
     8701 caccgggact tcgcccttct cttcatgtgc ttcatacgcc gcgtcaatca gctgcacacc
     8761 atagcgatga taaaattcac gcgctttctg gttgtatacg ttcgcgaaga aactcagatg
     8821 cgtttgcgga taaaccggcg caggatcagc aaccggttta cggctgccgc gctggtaact
     8881 ggcaagacgc gcagcatcca gcatgtcagc agcttcacgg cggaactggt ttaacagact
     8941 gtttggtaca aacagcgccc ccggcaaatt aatttgcaca tcgcgggcgt aatagagggt
     9001 ttgccccagt tttgccagac catccttcag attgttcatt gctttttcgg cgttattggc
     9061 ttcgtcgaac tgcccgtcca gcgtatgcgt gatgctgacc ccctcttcac tggtgagggt
     9121 cagaatcagt tgttcctgcc agccgcccag ttcaatgtct accgccaccc gacgttcgct
     9181 ggaggttttt gtcagtgcct gctgccagtt atgatcaaga ttacggttta gtgggtgatg
     9241 tggacgaatt ttgtgcaaat ctgctggcat ttcattgggc cagacgcggt actgattttc
     9301 tccggttttc tcgaccgtat tggcacgaaa accgacgact tcacgtttaa tcatcacgtt
     9361 caggccatca ccatttgcca gtggctcggt gacggcaaca tcgagatgat ctttcgccac
     9421 tttcaatact tcgcctaccg gcaggccgat aaatttcggc gaatcgaacg cgccaatatc
     9481 gcctttacgg gcattcacaa aataatctgt gctaccacgg tggaaagtct tttccgtcga
     9541 tggaacaaag aaatgttcag tacgacctga tgaagcgcgc gccagatcgc cacgttcttc
     9601 aataatggca tcaagcatct ggcgataatg ggcggtgata ttcttcacgt agctcatatc
     9661 tttgtaacgc ccttcaatct tgaaggagcg tacgccagca tcaatcagcg cgccgaggtt
     9721 ggcagtctga tcgttatctt tcatcgacag cagatgtttt tcataggaaa ccacccgccc
     9781 ctgatcgtct ttcaatgtgt atggcaaacg gcacgcctgc gagcaatcac cacggttggc
     9841 gctacgccct gtttgcgcat gagaaatgta gcactgaccc gaataggcca cgcacagtgc
     9901 cccatgaata aagaattcaa tggtcgcgtc cgtggcctgg tgaatcgcgc ggatctgctc
     9961 aagattcagc tctcgcgcca gcacaatctg cgtgaagcca acatcagaga ggaacttcgc
    10021 tttttctact gtacgaatgt cgcactgcgt actggcgtgc agttcaatcg gcggaatatc
    10081 aagttccaga atccccatat cctgaacaat cagcgcatcg acaccggtct ggtagaggtc
    10141 ggtaatcagc cgttgcgcgg gttccagctc atcatcatgc aaaatggtgt taagcgtgac
    10201 gaaaattttt gcaccataac gatgggcaaa cggcaccagc tcggcaatat ctttcaggct
    10261 attactggca ttatgacggg caccaaaacc agggccgccg atataaacag catcggcacc
    10321 gtgcaaaata gcttcgcggg caatggcggc atcgcgtgcc gggcttaaca gttcaagtcg
    10381 atgagaagat acggtcacat tcttatccag tagctgaaaa atggcggcta ttttagccac
    10441 aagcgcgacg ttgcgaaata gttttcccgc ttaactgcgc gggtaatgga tgagggaatg
    10501 aaaatgtacg gtttgctccc caccattacg atagatatgc gtgacgtctg cagcaaatcg
    10561 aacgccttcc ccacaattaa gggtctgcca ttccccatca acgcacagat caagttgtcc
    10621 atcaatgacg acgacatgtt cgattacccc cttctcatgg ggcgtcgatt cgcttattgc
    10681 accagatgcc atctgaatcg agaagtgttc gaagcagagt tgtggatcat aaggaaacag
    10741 cgaagtgatc accatcgcct gctgttgtgg atcataaact gaaggcgtag cggactgcgg
    10801 cggagaaata aatgttgaaa atggtacgtt caacccggtg gcaattttcc ataatgtcgc
    10861 gaccgtcgga ctggactcat tacgctcaat ctgccctaac attgctttag aaacgcccgt
    10921 cgcttctgcc agtcgcgaaa gactccagcc gcgctgctgg cgtagttgtt taagtgtggt
    10981 ggataaaaag cgagcgagat tttccatgcg actcctccgg caaaacggaa gtttatcact
    11041 tgtgcgttat aacggacaaa tgctacggtg cctgtacgct ataacgcacg aggtgactat
    11101 gcgtctgttt tctattcctc cacccacgct actggcgggg tttctggcgg tattaattgg
    11161 ctacgccagt tcagcggcaa taatctggca agcagcgatt gtcgccggag ccaccactgc
    11221 acaaatctct ggctggatga cggcgctggg gctggcaatg ggcgtcagta cgctgactct
    11281 gacattatgg tatcgcgtac ctgttctcac cgcatggtca acgcctggcg cggctttgtt
    11341 ggtcaccgga ttgcagggac taacacttaa cgaagccatc ggcgttttta ttgtcaccaa
    11401 cgcgttaata gtcctctgcg gcataacggg actctttgct cgtctgatgc gcattattcc
    11461 gcactcgctt gcggcggcaa tgcttgccgg gattttatta cgctttggtt tacaggcgtt
    11521 tgccagtctg gacggtcaat ttacgttgtg tggaagtatg ttgctggtat ggctggcaac
    11581 caaggccgtt gcgccgcgct atgcggtaat tgccgcgatg attattggga tcgtgatcgt
    11641 tatcgcgcaa ggtgacgttg tcacaactga tgttgtcttt aaacccgttc tccccactta
    11701 tattacccct gatttttcgt ttgctcacag cctgagcgtt gcactccccc tttttctggt
    11761 gacgatggca tcgcaaaacg caccgggtat cgcagcaatg aaagcagccg ggtattcggc
    11821 tcctgtttcg ccattaattg tatttactgg attgctggca ctggtttttt cccctttcgg
    11881 cgtttattcc gtcggtattg cggcaatcac cgcggctatt tgccaaagcc cggaagcgca
    11941 tccggataaa gatcaacgtt ggctggcagc tgccgttgca ggcatttttt atttgatcgc
    12001 aggtctgttt ggtagtgcta ttaccgggat gatggctgcc ctgcccgtaa gttggatcca
    12061 gatgctggca ggtctggcgc tgttaagtac cattagcggc agtttgtatc aggcgctgca
    12121 taatgagcgt gagcgcgacg cggcggtggt ggcatttctg gtaacggcaa gtggattgac
    12181 gctggtcggg attggttctg cgttctgggg attaattgcc ggaggcgttt gttacgtggt
    12241 gttgaattta atcgctgaca gaaaccgata ttgacatcct ccacgccctg aaggacgagg
    12301 atccctgcta cgttcaggct gtcgcctgaa tcatttcggt gggttcctgc ttcaacgggc
    12361 ggcctgactg caccatctct ccacaggcaa gaacggcgtg ccccgccgct aaaatgttac
    12421 gagcgccatt tacatcggcg ttcgctgtat atccacatac ctggcatctg aatttacttt
    12481 gtgacaggcg
//
LOCUS       DAZGOB010000140        12253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGOB010000140 DAZGOB010000000
VERSION     DAZGOB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.140"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(85..987)
                     /gene="yihR"
                     /locus_tag="ACHLIF_004179"
     CDS             complement(85..987)
                     /gene="yihR"
                     /locus_tag="ACHLIF_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO9608079.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1125..2366)
                     /gene="yihS"
                     /locus_tag="ACHLIF_004180"
     CDS             complement(1125..2366)
                     /gene="yihS"
                     /locus_tag="ACHLIF_004180"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO9608080.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2383..3261)
                     /gene="yihT"
                     /locus_tag="ACHLIF_004181"
     CDS             complement(2383..3261)
                     /gene="yihT"
                     /locus_tag="ACHLIF_004181"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO9608081.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3285..4181)
                     /gene="yihU"
                     /locus_tag="ACHLIF_004182"
     CDS             complement(3285..4181)
                     /gene="yihU"
                     /locus_tag="ACHLIF_004182"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO9608082.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4349..5245
                     /gene="yihV"
                     /locus_tag="ACHLIF_004183"
     CDS             4349..5245
                     /gene="yihV"
                     /locus_tag="ACHLIF_004183"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO9608083.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5279..6064
                     /gene="csqR"
                     /locus_tag="ACHLIF_004184"
     CDS             5279..6064
                     /gene="csqR"
                     /locus_tag="ACHLIF_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9608084.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6163..6762
                     /gene="yihX"
                     /locus_tag="ACHLIF_004185"
     CDS             6163..6762
                     /gene="yihX"
                     /locus_tag="ACHLIF_004185"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO9608085.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6756..7628
                     /gene="yihY"
                     /locus_tag="ACHLIF_004186"
     CDS             6756..7628
                     /gene="yihY"
                     /locus_tag="ACHLIF_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO9608086.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7625..8062
                     /gene="dtd"
                     /locus_tag="ACHLIF_004187"
     CDS             7625..8062
                     /gene="dtd"
                     /locus_tag="ACHLIF_004187"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO9608087.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8107..9048
                     /gene="fabY"
                     /locus_tag="ACHLIF_004188"
                     /gene_synonym="yiiD"
     CDS             8107..9048
                     /gene="fabY"
                     /locus_tag="ACHLIF_004188"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO9608088.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(9112..10020)
                     /locus_tag="ACHLIF_004189"
     CDS             complement(9112..10020)
                     /locus_tag="ACHLIF_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9608089.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            10249..10560
                     /locus_tag="ACHLIF_004190"
     CDS             10249..10560
                     /locus_tag="ACHLIF_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9608090.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            10561..10851
                     /gene="nadS"
                     /locus_tag="ACHLIF_004191"
     CDS             10561..10851
                     /gene="nadS"
                     /locus_tag="ACHLIF_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO9608091.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            11456..11674
                     /gene="yiiE"
                     /locus_tag="ACHLIF_004192"
     CDS             11456..11674
                     /gene="yiiE"
                     /locus_tag="ACHLIF_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO9608092.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            11893..12135
                     /gene="yiiF"
                     /locus_tag="ACHLIF_004193"
     CDS             11893..12135
                     /gene="yiiF"
                     /locus_tag="ACHLIF_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO9608093.1"
                     /translation="MNSLAGIDMGRILLNLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT         3014 a         2957 c         3221 g         3061 t
ORIGIN      
        1 ttaacttaat aaattgaaga tacataatgt tgcctttagc gagtgaaatg ttcttcaatg
       61 attttgactg ctaatcaata caagttattt tctctgtccg taaatgttaa aaaacaggcg
      121 gtgcggtttt cctggttcca gtaataataa gtcgatacct gaattaaagg cgttaggcgg
      181 gcaactcata ggttcaactg ccagtccctg acgctgtaat ttttcaccgc tataaatttg
      241 tacccagggt tgatcgcaac aaaggctaac cgaaagcgcc tgttgtggat gggtaatggt
      301 tatttcccac agatcattag cggttttaaa ggtgtggtcg atttttgtcg cggctattgt
      361 tttcgcctgc gtgaaattta aatccagctc atcaacgtgg tgaagcgttg tcggatttac
      421 gtgttcatca acggcataaa cctggtttgc cggaagctgg aacagatatt catcaacaga
      481 tgttaagttg caggttaaat aagtatgaat acccacgcca taaggcgctg cgacagtgcc
      541 gatattctgg cttgcgatct caacggaaag acccgtgtgt gcgttgagcg aataaacaac
      601 ttgtgaagcc agcataaacg gatagccgta actgggcggt agaaaagctg tcagggtaac
      661 gctggtggcg gttaattcac taatttgcca gtctcgccag gcaagcagcc cgtgaatagc
      721 ggcttttgaa ctatgttcat taatgggtaa ctgatattcc tgcccctgat agcggtaaca
      781 gccgttggcg atccggttcg gccacggaat aagcacttta cccagatgcg ccagcggcat
      841 ctcttcgggt ttgtgcggga tgactaaatg acaaccctga aaggttaatt cagccagacc
      901 agcaccgacg gtcacaatgg tcgcgtgata atcccccgcg gcaagtgaaa cggtctgtcc
      961 actgcaatgc atattggtga tttgcataca aggcacctta attaacgaca tacagcctcc
     1021 cgtgaggctg atgtcaaacc caaaaactgc accggacggt cccctcgccc ctttggggag
     1081 agggttaggg tgaggggaaa acccgcaccc tattttcaga tcagttattt cgcattaata
     1141 tccagcagac ccgctgcaac cgctggagcc atgccgggcg ctaacgggat acgcgggatc
     1201 accaggcaat gcagcaggtg ataaatatcc tgtttgccgt cccagacttt ggtggtgacc
     1261 ttattgtccg catccagctc ctgccaccag gaaccatttt catagtccat caggtactta
     1321 atgcagtact cccaccatgt ttgataccag gtttcatact ggcgatcgcc agtgacggtg
     1381 tagagcgcgt aggccgtacc cattgcttcg acgataggcc aacgtacacg ttcgcggacc
     1441 accggttttc cttcccagtc aacggtataa acaatcccgt ccgcgccatc gggtgcccag
     1501 gcatcgcgca cggtggcgtt aaacagacct ttggcatctt ctagcagcca tgctggtggt
     1561 tgttcgcaac gggcttccag ggcagcatgg atgtgcagca ttaaacggcc ccattcgatc
     1621 cagtggcctg gtgtacctcc gaacgcgcgg aagcgatgcg ccgggttatc tttgttgtaa
     1681 tccggcagcg gattccactg ggtatcgaag tgttcgttaa cgcgataatg attatttctg
     1741 gcgacgtcgt ggataatcac ggaagccacg cgaatcgcgc gatccagcca ttttttgtcg
     1801 tgagtgacgt cataaacaat caagaaagct tccaccgcgt gcatattggc attgccgccg
     1861 cggtactctt cggttttgct gaaggcttcg tcccaggatt ccaggcacat ctgctcttct
     1921 tcgctccaga aatatttctc gataatttca atggtgtaat cgagcagctt gcgcgcttcc
     1981 gggtgacccg ttgtgacggc gctggcggca cccagcagag caaagaagtg ttgatagccc
     2041 tgtttggagg catccaccac gccctcgtca tttacgcagg cataccagcc gccatatttt
     2101 ttatcacgca gtgcgccgtt catggctttg ataccgtgat caaccaacga gtaagcgcca
     2161 ggtcgaccca tcgccgcagc aacggaataa acgtgtaaca tacgagcggt gatccacaga
     2221 tgggtgccca tctcttcttt gatttgccct ttattgccta accagccaaa accagtcggc
     2281 actactgaat ttttaccaaa atcaaagatg cggtcggttt cctgttccag ccaacggttg
     2341 tggcttaggg tgttaaacca tttcattcct tttatcctca tcttagcggc gtttagccat
     2401 catttcgtcg acgatatcgc caagttgttg taatttcggt gcgcaaacgt cacgcagcat
     2461 cagctcgttg tctggtaaac cgacgaccga tgcccagact gcacggcctg ccaggaatcc
     2521 cgatgcgccc gccgtcattg ccacgcgtac ggcacgcggg aacagttttt cgtcgacacc
     2581 ggaagagagg atcacccatg gcatattgat atggtcattc agacgttgtg aagcacagag
     2641 aagctcttgt tgcggacctt tgccataaag cggcatttca actttgtaga ggtcagcgcc
     2701 actgtcgccc agctctttgg cggcatcgat gatcgcttgt tcgcgatcga atttatcgcc
     2761 acgacgcggt gggcggacga ctggctcaat gatgcttacc agaccgtgtg agtggcacag
     2821 ttcgttgaac tctttcacca tatccagacg ttgctgcgca tcttcatcac tacgccacag
     2881 caccagcagt tttaaggctt tgccaccgtc ttgtttgatc tgtagcggat tgattttgcg
     2941 gtcaataacc acgctatcga ccggaatacc gttgccagga atgaactcat cggcggcgac
     3001 aatcatggcg caacttttgg caatcgcgtt ttgctcaacc acctggcggt agcagaattg
     3061 ttgatctacc agaatcgccg aggcataagg cgagagggcc tttgcagcgt taactttgaa
     3121 atcagttaaa acgctatcgg ctaccggagc gggtgcccca gccgcggcaa acatcatgcg
     3181 catggcttcg cgctgatcga ccgccagcat ggcaaaaccg cccgatgcgc gcgtaatgtc
     3241 gttgatggtg tacttattca ttcatttatt cctttatcca gtcgttacat ttttactttg
     3301 gcagtcatcc cggcactgac acggacctgt tccagaatgg cggaccagtc ctggcgaccg
     3361 cgacccgctg cgcgcgcctg gctataaacc tcccgtgagg cggcccccag cggcattggc
     3421 acatgcagct ggttggcgac atcaagggcg atgccaagat ccttatgggc aagatcgatc
     3481 atgaaggcgg gagaaagatc tccgctgagg actttgtttg gccaggaagt ggtgaagtga
     3541 cctttaccgg cggcggtacc gctcatcact ttgacggcaa catcgaaggg aagattcagg
     3601 gcttcgcaca aaacggcggc ttctgccgaa agcgcattga gcgcgatgct catgtagttg
     3661 ttgatgagct taacgcggat ccccatgccc ggaccgccag cgttgatcaa ctcactgccc
     3721 atcgccatca ggatcggtgt ggcacgttca acttgttcag cggtgccgcc agccagcagt
     3781 aacagagtac cggtaatggc atttgcagaa gtacggccta ccggaacatc catcatgctg
     3841 aagcctttgg cttgcatatc ggcaatcaat ttatcggttt gcagcggatg gatggtggac
     3901 atatcaatga ccagcgcatc ggtagataag ccttcgcaaa cgccgttttc accgaacaac
     3961 acgttgcgca ccagatcgcc attcggcaac atggtaatga taaattcggc atctttagcg
     4021 gcctgcgccg ggttggcggc gggagtcgca cctttgtcta ccagatgccg cacagcttcg
     4081 gcgttcacat caaagacgcg aagttgatgc ccttgctgca ataaattgct cgccattggc
     4141 gaacccattt gtcctaaacc gataaacgcg attgctgcca taaccctctc ctgaatacag
     4201 tgatgtcact ttttgtcatt tatgacatgc tttgcttgtc tgtttttgat cgtatttgta
     4261 atttatcgtc aaaaaattga cagccgtcac tttttaaaca attggtgaaa ttaaaatgaa
     4321 cgcatcccaa aatgtttaag gaatgaccat gattcgtgtt gcttgtgtag gtataaccgt
     4381 gatggatcgc atctattacg tggaagggtt accgacggag agcggtaaat acgtggcgag
     4441 aaattatacg gaagttggtg gcgggccagc ggcgacggca gcggttgcgg cggcaaggct
     4501 gggggcgcag gtcgatttta ttggtcgcgt aggtgatgac gacacgggca acagcctgct
     4561 ggcagagctg gaatcctggg gcgttaacac ccgttacacc aaacggtata accaggcgaa
     4621 atcttcgcaa tccgccatca tggtggatac caaaggcgag cggataatca ttaactaccc
     4681 cagcccggat ctgctgcctg acgcagagtg gttggaggaa attgatttct ctcagtggga
     4741 tgttgtgctg gcagatgtac gctggcacga cggcgctaaa aaagccttca ccctggcccg
     4801 tcaggcgggt gtgatgaccg ttctggacgg ggacattacg ccgcaggata tcagtgagct
     4861 ggtggcatta agcgatcacg cggccttttc agaaccgggt ctggcgcgct taacgggcgt
     4921 gaaagagatg gccagtgcgc taaaacaggc acaaacgctc acaaatggtc atgtctatgt
     4981 gacccagggt agcgcaggct gcgactggct ggaaaatggt gggcgtcagc atcaaccggc
     5041 cttcaaagtt gatgtggtag ataccacagg tgcgggtgat gtttttcacg gcgctttggc
     5101 ggtggcgctg gcaacaagtg gggatttagc ggagtcagtc cgcttcgcca gcggtgtagc
     5161 ggcgttaaaa tgcacacgtc ccggtggacg agccgggatc cctgactgtg atcaaacccg
     5221 atcttttttg tcactttttg tataaaatgc cagggtgatg gtttttcgag gaattttcat
     5281 gagccttacc gaactaaccg gtaacccgcg gcacgaccaa ctcctcatgc tgatcgccga
     5341 gcgtgggtat atgaatattg atgagctggc aaatctgctg gatgtctcca cgcagacggt
     5401 ccgccgggat attcgtaaat taagcgagca aggcctgatt acgcgccatc acggtggcgc
     5461 gggtcgggct tccagcgtcg ttaatacggc gttcgagcag cgtgaggttt cgcaaaccga
     5521 ggaaaaaaaa gcgattgccg aagcggtggc agactatatt cctgatggtt caacaatatt
     5581 tatcaccatt ggtacgactg ttgagcatgt tgcccgggcg ttacttaacc ataatcattt
     5641 gcggataatc accaacagcc tgcgtgtggc gcatattctt taccacaacc cgcgctttga
     5701 agtgatggtg cccggcggta cgttgcgctc tcataatagc gggatcattg gcccttcagc
     5761 ggcgtccttt gtggctgatt ttcgtgctga ttatctggta acaagcgttg gggcgattga
     5821 gagcgatggc gcgttgatgg agtttgatgt aaacgaagct aacgtggtga aaacgatgat
     5881 ggcgcacgcg agaaatattc tgctggtcgc cgatcacact aagtatcatg cttcggcggc
     5941 ggttgaaatt ggtaacgtgg cacaggtcac tgcgctcttt accgacgagc tgccgcccgc
     6001 tgcgctaaaa tcacgcttac aagacagcca aattgaaatc attcttcccc aggaagacgc
     6061 gtagattttt gtgaccgtta acctggcttc ataccttgcc acatagccaa acccatcctt
     6121 tcccgctaca gttaatttct tgtggcgcga aaggaggcaa aaatgctcta tatctttgat
     6181 ttaggtaatg tgattgtcga tatcgacttt aaccgtgtgc tgggagcctg gagcgattta
     6241 acgcgtgttc cactggctac gcttaagaag agtttccaca tgggggaggc gtttcatcag
     6301 catgagcgtg gggaaattag cgacgaagcg ttcgcagagg cgctgtgtca tgagatggcc
     6361 ctgccgctaa gctacgagca gttctctcac ggctggcagg cggtgtttgt tgcgctgcgc
     6421 ccggaagtga tcgccatcat gcataaactg cgtgagcagg ggcatcgcgt ggtggtgctt
     6481 tccaatacta accgcctgca taccaccttc tggccggaag aatacccgga aattcgtgat
     6541 gctgctgacc atatctatct gtcgcaagat ctggggatgc gcaaacctga agcacgaatt
     6601 taccagcatg ttttgcaggc ggaaggtttt tcacccagcg atacggtctt tttcgacgat
     6661 aacgccgata atatagaagg agccaatcag ctgggcatta ccagtattct ggtgaaagat
     6721 aaaaccacca tcccggacta tttcgcgaag gtgttatgct aaaaaccatt caggacaaag
     6781 ccaggcaccg tacccgtcca ctatgggcct ggctaaaact actctggcaa cgtattgatg
     6841 aggacaacat gacaaccctg gcaggtaacc ttgcctatgt gtcgttgctc tcattagtgc
     6901 cgctggttgc cgttgttttt gcgcttttcg ccgcttttcc catgttttcc gacgtcagca
     6961 ttcagttacg tcactttatt tttgccaact ttctgcctgc tactggcgat gttattcagc
     7021 ggtatatcga acaatttgtt gccaattcca acaagatgac cgccgttggg gcgtgcgggc
     7081 tgatcgtcac cgcgttattg ctgatgtact ccatcgatag cgcgttgaat accatctggc
     7141 gcagtaaacg agcgcgacca aaaatttact cgttcgccgt gtactggatg attttaacgc
     7201 tggggccgct gctggcaggg gccagtctgg cgatcagttc ctatttgctc tccctgcgct
     7261 gggcgagcga tctcaatact gtcatcgata acgtgctgcg tatttttccg ctgctgttgt
     7321 cgtggatctc cttctggttg ctgtacagca ttgttcctac catccgcgta cctaaccgcg
     7381 acgcgattgt cggcgcgttt gtcgccgcgc tcctgttcga agcaggaaag aaaggtttcg
     7441 cgctttatat caccatgttc ccgtcatatc agctcattta cggcgtgttg gcggtgatcc
     7501 ccattctctt tgtttgggtc tactggacat ggtgtatcgt cttgcttggc gcggaaatta
     7561 ctgtcactct cggggaatac cgcaaactaa aacaagcagc tgaacaagaa gaagacgacg
     7621 aaccatgatt gcattaattc aacgcgtaac ccgtgccagc gtcaccgtgg agggagaagt
     7681 gacgggcgaa attggcgcgg gacttttggt gttattgggt gtcgaaaagg atgacgacga
     7741 acagaaagca aaccgtctgt gcgagcgtgt gctcggctac cgcatcttta gcgatgccga
     7801 aggcaagatg aatctcaacg tgcaacaggc gggcggcagt gtactggtgg tttcccagtt
     7861 taccctcgcc gcagataccg aacgggggat gcgcccaagt ttctctaaag gcgcatcacc
     7921 ggatcgcgca gaggcgttat atgattattt cgtcgaacgc tgccgccagc aagagatgaa
     7981 cacgcaaaca ggacgcttcg ctgccgatat gcaggtatcg ctggtcaatg atggccccgt
     8041 gacattctgg ttgcaggtat gagccagctt ccagggttgt cacgggaaac aagagagagt
     8101 atcgctatgt atcaccttcg ggttccacaa acagaagaag agttagagcg ttactatcag
     8161 tttcgctggg aaatgttgcg taagcccctg catcaaccaa aaggttcgga acgcgacgcg
     8221 tgggatgcga tggcgcatca ccagatggtc gtcgacgagc agggtaatct ggtggcggta
     8281 ggccgattgt atattaatgc cgacaatgaa gcgtccattc gctttatggc cgttcacccg
     8341 gacgtgcagg acaaagggtt aggaacgctg atggcgatga ccctggagtc ggtggcgcgt
     8401 caggaaggcg ttaagcgcgt gacctgtagc gcccgtgaag acgcggtgga gtttttcgcc
     8461 aagctggggt ttgttaatca gggagaaatc accacgccaa ccaccacgcc gattcgccat
     8521 tttttgatga ttaagcccgt cgccactctg gatgatattt tgcatcgcgg cgactggtgc
     8581 gcgcagctgc aacaggcgtg gtacgaacat atcccgctta gtgaaaaaat gggcgtgcgt
     8641 attcaacaat ataccgggca aaaatttatc accaccatgc cggaaaccgg taatcagaat
     8701 ccgcaccata cgctgtttgc cgggagttta ttctcactgg caacgctcac cggttggggg
     8761 cttatctggc tgatgctgcg cgaacgccat ctcggcggaa cgattattct ggcggatgcg
     8821 catatccgct acagcaagcc gattagcggt aaacctcatg cggtagccga ccttggtgcc
     8881 ttaagcggcg atctcgaccg tctggcgcgc ggacgaaaag cacgggtgca gatgcaagtt
     8941 gaaatctttg gcgacgagac gccgggtgca gtgtttgaag gcacgtatat cgttctgccc
     9001 gcgaagccat ttggcccgta tgaagagggc gggaacgaag aagagtaggg gcgttttact
     9061 tgactgaaaa ggttttcaat ctgaaaacct tttcagggcc caggtccgaa gttaccttcc
     9121 cgctctccag gcaacatccc gtagtgccga tcgcgcggcc tcactgatac cacctaactg
     9181 aaaaaatccg tggataacgc caaggtagcg ttggcaggtg cattgcactc cttgccctgt
     9241 catgcggtga tacaacgctt caccttcatc gcaaagtggg tcgtattcag cggtaatgat
     9301 atgtacaggt ggcaagccat tgaaatcatc gcgccaaaga gggctggctt caggatgcct
     9361 gcgttcaatg cctggcagat acatctcaaa gccgctcagt aaagtgtcgc gtgtgatgat
     9421 gtaatcgtga ccattgcgta tgtagctttc gaaatgcgca gtcgcatcaa gcataggata
     9481 aattaagatg agttgtgccg gttgccactc gccagcacgc tttaatcgca aagcagttac
     9541 cagcgccaga tgtccgcccg cactgtcacc ggcaagagtt atacgttgtt tatcaacgcc
     9601 gagtcgttcg gcgtgttgcc agactatttc tgcacctcgt tgcgcatcat catgggcagc
     9661 ggggaaagta tgttccggtg ccagccgata ctggacggca ataacccgac attgcccgta
     9721 ataggctaac tggcgcagct ggttgtcgtg agttgcgaac ccgccgctaa caaaacagcc
     9781 accgtagtaa taaattatcg tcgggagtaa tgtcggggca ttcagcggcg acactattcg
     9841 taaggtcatc ccttcaagtt cgatagtctg aatatctacc cgcgtttctg tctcacccgc
     9901 aaggacagcg ctggcgatat agccctccct gcgctgcgca atactttgtt gacgcgatga
     9961 ggggcgaccc gcagcgataa acttttcgac taactctgca attccttttt ccagtgccat
    10021 agtagaactt attttgctgt atgaatgtac agtcttatac acccgttttt gctggatgga
    10081 tacagggaaa ttcacgggcg tgaaaaatct ggaaagcgcc tcgcaaatct aaagacaaac
    10141 ccggcagaga cgtggttttg ctggagcact atcagtaagc tagtggtttc aagggtacgt
    10201 caatgacgta caaaaaggat tgttactcta agccaatggc gtataattat gctcttcatc
    10261 gaaacggaaa tttttactga agatgttcaa aaactgctga ccgacgatga attcagtcgt
    10321 tttcagtttt tcctggcctt gaatcctgac tatggtgagg taatacctga aactggtggg
    10381 ttaagaaagg ttcgatgggt gtcaggagga aaggggaaac gtgctggtgt cagggttatt
    10441 tactttcatc aggttaaaca ttatgagatc agattactgc ttatttatcg taagggtatt
    10501 aaagatgatc tgtctcccca ggaaaaagca atgctccggt tgttaaatac gaggtggtaa
    10561 atggataagg cactttttga gcggttgact cacagcatgg cccagatgaa tgaaattatt
    10621 gaaggaacgc gacagccttc gcggactttt gaagttgatg ctatgaaaat caaagaaata
    10681 cgccgcgctt cagggttgtc acaatctaaa tttgccgatc ttatttctgt aagcgtagac
    10741 acgttgcgta actgggagca ggggcgacgt tcaccgaccg ggcctgcaaa ggctttgctg
    10801 cgagcgattg ccaatgatcc gcaacatgtg ctgcaagcac ttaaccgtta gaggcatggt
    10861 tatgccatac agaaagcact agcattcaga actcctgtga aagcggcata gtggtcgcct
    10921 gcccaggagt tttgctatga cccgtaatcg tgctgaacgt cgccacccta tgatgcgatt
    10981 aaaaaaactc atcgtcgcgc tgatgattga cgtcaatgct atcggatgat accggggggt
    11041 tccggaagga attccttgtt tcccgaactg ttaccaacaa aattataacg atgaacaagt
    11101 aaagaccggt tggtggcagt actgaccggt ctgtgatgga tgattaattt caccttaaat
    11161 ccgggtgctg ttaggatgct gacatttttc gggtacacca ttcagattat gcaaataaag
    11221 atccaatcaa aaaataaaca tccctgaata taatatattc atagcatcca taactcatcc
    11281 tgggcgataa ctaaaccatt tttgagtaat aaatgaaaca actgcaacac tcttattatt
    11341 tctggaaaac gcctcgcaaa aacaaaacaa catgcaaaaa tccgcttttt ttaaatgatt
    11401 ttccggcgta attttcgcac atacattaat gagtaatata ggtaaggtgg tggtaatggc
    11461 gatgaatacg gtttttcttc atttatcaga agaggcaatt aaacggctga acaaacttcg
    11521 gggctggcgt aaggtttcac gatctgcaat tttacgcgaa gcggttgagc aatatctgga
    11581 gcggcagcaa tttccggtgc gtaaagcaaa gggaggcagg caaaaggggg aggtagttgg
    11641 tgttgatgat cagtgtaagg agcataagga atgaaaaaaa tttttcattc ttgaatataa
    11701 aaaaacagat gcccttattc tggtattaat acaaggctgt tttacttgaa cttataataa
    11761 ctgcaactgt tacatcgtat ctggaaaacg cctcgcaaaa ataaaaaatg atgcgtaaat
    11821 gagctttctt taaagattcg gcaagggtac ttttctctcc atagattcaa aaatgatatt
    11881 ggcgagatat ttatgaactc tctggcaggt atagatatgg gcagaatttt actcaattta
    11941 tcgaatgagg tgattaagca acttgatgat ctcgaggtgc agcgtaatct tcctcgtgcg
    12001 gacctattaa gggaagcggt agatcaatac ctgataaatc aatcgcaaac agcaagaacc
    12061 agtgttcctg gcatctggca agggtgtgag gaagatggtg tcgaatatca gcgtaagctg
    12121 cgcgaggaat ggtaatggga aaaaatgatg tcaatcagat tgctgacaac gtgcgcgttg
    12181 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aagtcatgca aattcaatat
    12241 attgaagttt att
//
LOCUS       DAZGOB010000141        12016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGOB010000141 DAZGOB010000000
VERSION     DAZGOB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.141"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..197
                     /locus_tag="ACHLIF_004194"
     CDS             <1..197
                     /locus_tag="ACHLIF_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO9608094.1"
                     /translation="PYGVLDSKGFGVFLAMICATFIISIISYNFIEKPSVSIGHAIAR
                     RMQKKSDVSSIKTESVREAP"
     gene            complement(442..1599)
                     /gene="intS"
                     /locus_tag="ACHLIF_004195"
     CDS             complement(442..1599)
                     /gene="intS"
                     /locus_tag="ACHLIF_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO9608095.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMRGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(1761..1835)
                     /locus_tag="ACHLIF_004196"
     tRNA            complement(1761..1835)
                     /locus_tag="ACHLIF_004196"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1800..1802),aa:Arg,seq:cct)
     gene            complement(1911..2843)
                     /gene="yfdC"
                     /locus_tag="ACHLIF_004197"
     CDS             complement(1911..2843)
                     /gene="yfdC"
                     /locus_tag="ACHLIF_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO9608096.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3137..3892
                     /gene="mlaA"
                     /locus_tag="ACHLIF_004198"
     CDS             3137..3892
                     /gene="mlaA"
                     /locus_tag="ACHLIF_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO9608097.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4074..5132)
                     /gene="yfdF"
                     /locus_tag="ACHLIF_004199"
     CDS             complement(4074..5132)
                     /gene="yfdF"
                     /locus_tag="ACHLIF_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFO9608098.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISNLFANKVDVDHPENKHKDFKDLEDNVEKRLQNRQAK
                     YQNEINQLSAPGVNFDDI"
     gene            complement(5497..6837)
                     /gene="fadL"
                     /locus_tag="ACHLIF_004200"
     CDS             complement(5497..6837)
                     /gene="fadL"
                     /locus_tag="ACHLIF_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO9608099.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            7209..7493
                     /gene="yfcZ"
                     /locus_tag="ACHLIF_004201"
     CDS             7209..7493
                     /gene="yfcZ"
                     /locus_tag="ACHLIF_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO9608100.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            7673..8983
                     /gene="fadI"
                     /locus_tag="ACHLIF_004202"
     CDS             7673..8983
                     /gene="fadI"
                     /locus_tag="ACHLIF_004202"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO9608101.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            8983..11127
                     /gene="fadJ"
                     /locus_tag="ACHLIF_004203"
     CDS             8983..11127
                     /gene="fadJ"
                     /locus_tag="ACHLIF_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO9608102.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            11330..11815
                     /gene="sixA"
                     /locus_tag="ACHLIF_004204"
     CDS             11330..11815
                     /gene="sixA"
                     /locus_tag="ACHLIF_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO9608103.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2986 a         2783 c         2977 g         3270 t
ORIGIN      
        1 aaccgtacgg ggtattggat agtaaaggat ttggtgtttt tctagctatg atttgcgcta
       61 cttttattat atcaatcata tcttataact ttattgaaaa accatcagta tccatagggc
      121 atgccatcgc tcgtagaatg caaaaaaaat cagatgtatc ttcaattaaa actgaatccg
      181 ttagagaagc accttgataa atagagcaat agccagatag cacctaaccg atttcgtact
      241 catagggttg tcactttacc aaactgagag gaaatacact gataacagtt ggttatgaag
      301 tactgggtga ggtggatgtg aatggtattt tgatgcattt cattaacctg tacgcctaag
      361 aaagcaaccc tgaaatctaa gacacacaag ctttgcactg gattgcaagg ctttgtgcac
      421 ttcgatagtg gttaaggtgg ttcactccac cttttcatca agccagtccg cccaccattg
      481 catcatttct ctgcgcttat cgagatactg agcatggttg taaataccgc gcacagatcc
      541 gccgttggca tgtgccagtt gcacttcaat agcgtcagca ggccattcgt gctcgttcat
      601 aatcgtgctg aattcatgcc tgaatccgtg accgctttcc agaccctcat agccgatttg
      661 tttgatcaca agtagcaccg cgttctcgca gattggcttc ttcttatcgt tgcgcccggc
      721 aaaaacaaat tctgatactg gtttggtgat ggagcttaac gtagtgagaa gttcaaccac
      781 ctggtccgac atcgggacaa catgaatttt gcgtcccttc atcacattgg cgtcaatggt
      841 gataatcctg ttttcaaaat cgacgttctt ccattgcatg gaacgaagct cttttgttct
      901 tagggctgtg tagcgtaaaa ccttggtggc aatgagcgat acgatacttc ctgaaaatgt
      961 tgccagtgct ttattgaatg ccgggatctg gtctgcagga agaaacggga agttcttctt
     1021 gcggtatccc ctcatggcgt cagcaaggtc aggtgccggg ttatatttag ccctgccggt
     1081 gacaatagcg taacggaaaa cctcgccgca tcttctgcgg gctttatttg ctcgctccat
     1141 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
     1201 aatttcaagg ccgccgatga taggcaaaat gtcgtcatca aacatttttg caagttcagt
     1261 cgcataccct actgaccata cttgcttctt gtgctcgtac cattccttgt aaatcgcact
     1321 aaaggaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc caaccgagat
     1381 gtctttcctc gcagtccatg ccttatccct tgcctcctgc aaagtcatta gcggatattt
     1441 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
     1501 cccggataca ggaacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
     1561 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggagggtaa
     1621 aattatttta tccacttttt acccgtcatg gggtgcggct gtcaacgatc tgacgcgaac
     1681 catgacgaac tgtgaatcta cggaaggctt gatattcagg ggattttgcg gactggtacg
     1741 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
     1801 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa
     1861 tggactgcgg ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgctggg
     1921 tttttatcat ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgcttta
     1981 cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa gataaaggtg
     2041 ccgccgcaga tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag
     2101 tgcagcgtgc cgttaaacac cagatagagg atttcaacag aaccgacaac gatgtgggtg
     2161 gtgtcaccca gtgcaataag ccaggtcatc aaaataatca ccacaatctt tgccgcaccc
     2221 gctgcaggaa acatccaaac catagtggcg atcagccagc cggaaatgat cgcgttggca
     2281 aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga
     2341 gtttcttcat tgaagatagg catatattca aatgcccatg cagcaatacc tgtcccgaga
     2401 atattaccca gcagcacgac gccccataat cgcataagta agccgacgtt gctcattgtc
     2461 ggtttttgca tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg
     2521 gccataatga cgataataaa accaaaggta taaccgagat tctccagtaa gaagctgcct
     2581 ggcactcctt ccagttcgac atgaaatatc ccttttgcca gtagcgaagc gcccatcgac
     2641 agacccgccg caatggctga ccacagtagc gccattgcgt cgcgttccag ctctttttca
     2701 ccatcctggc ggatatgctc atgaattgcc atcgcccggg aggggagtcg gtcttcatct
     2761 atttctattt ttttgccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt
     2821 tgatcaattt tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg
     2881 tagcggcttt tttgctcgca actggcggga gttactctga aaatgtagaa aaggctgcgt
     2941 ttgccttttt ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg
     3001 atcaaatcag caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca
     3061 cctaaacagg cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa
     3121 aaacagggag acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct
     3181 ggtggggtgt gcgagttccg gtacagatca gcaagggcgt tctgacccgt tagaagggtt
     3241 caaccgcacc atgtacaact tcaacttcaa cgtattagac ccgtatattg ttcgaccggt
     3301 cgctgtcgcc tggcgtgatt atgttccgca accggcgcgt aatggtttga gcaactttac
     3361 tggcaacctt gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca
     3421 ggggatggtc cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat
     3481 tgatgttgca gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag
     3541 tacgcttggt cattatggcg tgggttatgg gccttacgtt cagttaccgt tctacggtag
     3601 cttcacgctg cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg
     3661 gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa gggatcgaaa cccgcgctca
     3721 gctgctggat tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga
     3781 agcgtacttc cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa
     3841 tccgaacgca caagcgattc aggatgattt aaaagatatt gattctgaat aagaaacaaa
     3901 taaaaaaggt gagtcgcaaa actcaccttt tttgttgtta tccctcaatt accacaagta
     3961 aaaggagata aaccgacaat gacccggacg taatgatgta aaaatactga tgttcgtcac
     4021 tgactatttc cgatattcag gcgttgaaaa atcaaaaaac tggaaaaata attttaaata
     4081 tcatcaaaat taacacctgg cgcagatagt tgattaattt cattttgata tttcgcctgg
     4141 cgattttgaa gtcttttttc aacgttatct tcgagatcct taaagtcttt gtgtttattt
     4201 tcaggatggt caacgtcaac tttgttagcg aataaattag aaattatttc ttttacattg
     4261 tcgcgtctaa attttgcatc gtccacttga aaattatatc ttattgctcg acctaaacgt
     4321 ttaaaaactt cgttaggttc tctcagtctt tccccgttga gctcgtttac aacctgatga
     4381 tcggaacctg ttgcaaatga ttctgccatg atagctttac tgctttcaaa ccacgctgaa
     4441 gcattcctgg ctttttcact acaatattgg gtgagactat caccattaat acgaattcct
     4501 ctttgaatgt ggtagtcgtc ctgatcgtag taaccgagag ataagtaatc atttccgttt
     4561 ttatttttaa ttacaatagc gagagagtcg ccttttttat ctttaccgta ccaggtaaat
     4621 aagtccgttg gttcgctatt cccgctctca tcaaactccg ttaccgtatg tctgagtttg
     4681 tgtgatatct tatcgaagat aaaaccagga tctccatcat atatttcttt taatgcgctg
     4741 aagatcattt ccttgttatt gatatcatga gtcttctcaa ctatgttgtc attaatgata
     4801 gtacggacga gttctttgtc tttatagttc gcaggtaatt ctattggttc ttggttgtcg
     4861 ataccaatag taatatggtc tgtatcattg gagccaatat caatataagt aactgaggaa
     4921 ttattaatat taaatctata aggatcgtcg tttactcgta tggcgttttt tataagctca
     4981 agtagatgtt taatacaggt gcttattcct tccttaccat taaaaaggtt tttgagctct
     5041 tgtactaatc cattaacttc atcattgtta ttttcattaa tggattctgg gtaagttgcg
     5101 ctggtattgt tgattgaaat agatggtagc atgattttat tccattttga ttgatttgtt
     5161 acaaatatat attacctaat gaaatagact atcaaaaaac gctcatttat ttagaaccta
     5221 tctttaattt gagtgtatga gcgcacaata gccagggggg tgagtatcct ggaaattcag
     5281 aaaagtaaac tctttgagaa ggacgagcag aacgcctgaa aatatgcctt tcccttcaga
     5341 tttgtctgtg gataccgctt attgatttga aagggcaagc tgccatcagg ctgtttgcaa
     5401 aactcaggag gtaagcaatg aggacactac tttcggtgaa gtgggggagt taaagtcacc
     5461 tgctatgcag gtgactttat ccaggcgaac gcgttatcag aacgcgtagt taaagttagt
     5521 accgaacagc caggctttac cttcagactc gaactggtat gggccttcgt taattttcac
     5581 gctctgaccg tgcatataag aaacaccaac gtcgactgaa gcatctttat taaatgcgta
     5641 agtcgtacct gcactcagcc agaaacggtc ctggtccgga atggagatag aacgattctg
     5701 agccggaact gggctgtcat caaaggcgat accggtacgg aaggtccagt tatcatcgta
     5761 gtaataagtg gtacccaacg cgatgcggta agcatcttta aagccttcat gtttctggaa
     5821 cagcgtgtcg ccactggttg aggtcgcttt cagctgctgg aactgactcc agctggtgta
     5881 agccaagcta tagtgaatcg cccactgtgg atcaacacgg ttataacctg acacttccca
     5941 catttcaggc aggttcagcg tcagataacc cgattgcgtt gcgccacctg tcgcggtagg
     6001 aattggcaaa ccgtagttat taaacgcacg attaagatcg ctgctgtagt tacctttgaa
     6061 gtcaattttc acttcagaac ggtaggtcag tgcatagcgg ttatttttat ccagttcata
     6121 caggattccg gcgttccagc caaagcccca ctggttaccg ttcagatgag cgattttggt
     6181 attactgtcg ataccgttgg cggtagctgc caatgcttgc ccttgaggag tttgcccggc
     6241 aggagattgc ataatctgac cagcaaccaa ctgccccaga tcgcctgcga aacgttcaat
     6301 tttcgcgcga gcgtagacgg cgttgaaacc aagaccaaag ctccatgcat tatttaagcg
     6361 atacgcaccg cttaagttca ggttcatggt ttcaaggtca gttgtacccc cgacagagcc
     6421 gcctgcataa gtatcattaa actctgttgc cagaccatag ttagaggtaa tagaagcgcc
     6481 ccaaccaaat tggtcgttaa tcggtgcaac aaagtgcatg ttcggaaccc atgccgtagg
     6541 cgcgatgtta tcggctttca ggctacgacc agacggagac gttccgctga tattcacatc
     6601 cgggtcaata taaaccgcac ccgcagaaaa tgtcgggcgg tcaaacatgg taatcaatgc
     6661 ggggttacgg ctaacgttac ctgcatcatc ggcaattgcg ccttcccctg aataagcccg
     6721 gcccaggcca gaggaagaaa attcgtttaa ctgaaagcct gccgaccagg cctgggtgga
     6781 gataagtgcc actgcgactg cgagagcaga ctttgtaaac agggttttct ggctcatgac
     6841 cataacctca atgatttatt tttatacaaa ctatgttacg tgctgtaaca ggagcgcgaa
     6901 gtgtagggtc tgaggtacat cttagaaatc agaccagtgg cgagagtata ggtcggacca
     6961 gctggaatgt tgcaagtatg tttccatctt ttttcaaata tgatctaaga aacgcgatcc
     7021 agctggcaaa atttggaggt tgcgcactcg cggattcggg tgaattttgt tttagatcat
     7081 ttttaagtgt gatttcggtc acttatccga ttttgcagaa ttaacaactg gagcagcact
     7141 ttccggaagc gtaaaatatc cacaacgttt atctggcacc ttgggtgcac atacagagga
     7201 aatcaacgat gagtaaatgt agtgctgatg aaaccccggt ttgctgctgt atggatgttg
     7261 gcaccattat ggacaactcc gactgcaccg cgtcttacag ccgcgtgttt gcaagccgcg
     7321 cagaagcaga acaaacgctg gcggcgctga ctgaaaaagc ccgtagcgtg gaatccgaac
     7381 cgtgtaaaat caccccgact ttcactgaag agtctgacgg tgtgcgcctg gatatcgatt
     7441 tcacttttgc ctgcgaagct gaaatgctga tcttccagct gggtctgcgt taattcctct
     7501 cgggtacgta cgcccctgtc agttgctggc aggggcgttt tcttttcttt ctcctgtctt
     7561 cactgtgtca tagctcccac tttccttttt ctccgcttgg ctaaatgtaa aaaaatggtt
     7621 aagactatga tcaggtcaga ccactttatt tattttttta caggggagtg ttatgggtca
     7681 ggttttaccg ctggttaccc gccagggcga tcgtatcgcc attgttagcg gtttacgtac
     7741 gccttttgcc cgccaggcga cggcttttca tggcattccc gcggttgatt tagggaagat
     7801 ggtggtaggc gaactgctgg cacgcagcga gatccccgct gaagtgattg aacagctggt
     7861 ctttggtcag gtcgtacaaa tgcctgaagc ccccaacatt gcgcgtgaaa ttgttctcgg
     7921 taccggaatg aatgtgcata ccgatgctta cagcgtcagc cgcgcttgcg ctaccagttt
     7981 ccaggcggtt gcaaacgtcg cagaaagcct gatggcggga actatccgag cggggattgc
     8041 cggtggggca gattcctctt ccgtattgcc aattggcgtc agtaaaaaac tggcgcgcgt
     8101 gctggttgat gtcaacaaag ctcgaaccat gagccagcga ctgaaactct tctctcgcct
     8161 gcgtttgcgc gacttaatgc ccgtaccgcc tgcggtagca gaatattcta ctggcttgcg
     8221 gatgggcgac accgccgaac aaatggcgaa aacctacggc atcacccgag aacagcaaga
     8281 tgcattagcg caccgttcgc atcagcgtgc cgctcaggca tggtcagacg gaaaactcaa
     8341 agaagaggtg atgactgcct ttatccctcc ttataaacaa ccgcttgtcg aagacaacaa
     8401 tattcgcggt aattcctcgc ttgccgatta cgcaaagctg cgcccggcgt ttgatcgcaa
     8461 acacggaacg gtaacggcgg caaacagtac gccgctgacc gatggcgcgg cagcggtgat
     8521 cctgatgact gaatcccggg cgaaagaatt agggctggtg ccgctggggt atctgcgcag
     8581 ctacgcattt actgcgattg atgtctggca ggacatgttg ctcggtccgg cctggtcaac
     8641 accgctggcg ctggaacgtg ccggtttgac aatgagcgat ctgacattga tcgatatgca
     8701 cgaagccttt gcagctcaga cactggcgaa tattcagttg ctgggtagtg aacgttttgc
     8761 tcgtgaagta ctggggcgtg cacatgccac tggcgaagtg gacgatagca aatttaacgt
     8821 gcttggcggt tcgattgctt acggacatcc cttcgcggcg accggcgcac ggatgattac
     8881 ccagacattg catgaacttc gccgtcgcgg cggtggattt ggtttagtta ccgcctgtgc
     8941 tgccggtggg cttggcgcgg caatggttct ggaggcggaa taatggaaat gacatcagcg
     9001 tttaccctta atgttcgtct ggacaacatt gccgttatca ccatcgacgt accgggtgag
     9061 aaaatgaata ccctgaaggc ggagtttgcc tcgcaggtgc gcgccattat taagcaactc
     9121 cgtgaaaaca aagagttgcg aggcgtggtg tttgtctccg ctaaaccgga caacttcatt
     9181 gctggcgcag acatcaacat gatcggcaac tgcaaaacgg cgcaagaagc ggaagcactg
     9241 gcgcggcagg gccaacagtt gatggcggag attcatgctt tgcccattcc ggttatcgcg
     9301 gctattcatg gcgcttgcct gggtggtggg ctggagttgg cgctggcgtg ccacggtcgc
     9361 gtttgtactg acgatcctaa aacggtgctc ggtttgcctg aagtacaact tggattgtta
     9421 cccggttcag gcggcaccca gcgtttaccg cgtctgatag gcgtcagcac agcattagag
     9481 atgatcctca ccggaaaaca acttcgggcg aaacaggcat taaagctggg gctggtggat
     9541 gacgttgttc cgcactccat tttgctggaa gtcgctgttg agctggcgaa gaaggatcgc
     9601 ccatcttccc gccctctacc tgtacgcgag cgtattctgg cggggccgtt aggtcgtgcg
     9661 ctgctgttca aaatggtcgg caagaaaaca gaacacaaaa ctcaaggcaa ttatccggcg
     9721 acagaacgca tcctggaggt tgttgaaacg ggattagcgc agggcaccag cagcggttat
     9781 gacgccgaag ctcgggcgtt tggcgaactg gcgatgacgc cacaatcgca agctttgcgt
     9841 agtatctttt ttgccagtac ggaagtgaag aaagatcccg gtagtgatgc accgcctgcg
     9901 ccattaaaca gcgtgggaat tttaggtggt ggcttgatgg gcggcggtat tgcttatgtc
     9961 actgcttgta aagcggggct tccggtcaga attaaagata tcaacccgca gggcataaat
    10021 catgcgctga agtacagttg ggatcagctg gagggcaaag ttcgccgtcg tcatctcaaa
    10081 gccagcgaac gtgacaaaca gctggcatta atctccggaa cgacggacta tcgcggcttt
    10141 gcccatcgcg atctgattat tgaagcggtg tttgaaaatc tcgaattaaa acaacagatg
    10201 gtggcggaag tggagcaaaa ttgcgccgct cataccattt ttgcttcgaa tacgtcatct
    10261 ttaccgattg gtgatatcgc agctcacgcc acgcgacctg agcaagttat tggcctgcat
    10321 ttcttcagtc cggtggaaaa aatgccgctg gtagagatta ttcctcatgc gggtacatcg
    10381 gcgcaaacca tcgccactac ggtaaaactg gcgaaaaaac agggcaaaac gcccattgtc
    10441 gtgcgtgaca aagccgggtt ttacgtcaat cgcatcttag cgccttacat taatgaagct
    10501 atccgcatgc tgaccgaagg tgaacgggta gagcacattg atgccgcgct agtgaaattt
    10561 ggttttccgg taggtccaat ccaacttttg gatgaggtag gaatcgacac cgggactaaa
    10621 attattcctg tactggaagc cgcttatgga gaacgtttta gcgcgcctgc aaatgttgtt
    10681 tcttcaattt tgaacgacga tcgcaaaggc agaaaaaatg gtcggggttt ctatctttat
    10741 ggtcagaaag ggcgtaaaag caaaaaacag gtcgatcccg ccatttaccc gctcattggc
    10801 gcacaagggc aggggcgact ctccgcaccg caggttgctg aacggtgtgt gatgttgatg
    10861 ctgaatgaag cagtacgttg tgttgatgag caggttatcc gtagcgtgcg tgacggggat
    10921 attggtgcgg tatttggcat tggttttccg ccatttctcg gtggaccgtt ccgctatatc
    10981 gattctctcg gcgcgggcga agtggttgca ataatgcaac gacttgccac gcagtatggt
    11041 tcccgtttta ccccttgcga gcgtttggtc gagatgggcg cgcgtgggga aagtttttgg
    11101 aaaacaactg caactgacct gcaataagaa ggtcaaagct atatgaatcc gcgctgaatg
    11161 gcggagtgtt ggtcaaaata taaacgcata ttgactatac ttacgccatt gaggtaaaaa
    11221 acagcgtttc attcggtgaa tggataaggc acaatgccgg ccaccgtttt ctttctctgg
    11281 tttcagatga aagaaaacgg gcgaatctgg ttaacaaaag cggtgcaata tgcaagtttt
    11341 tatcatgcgt cacggcgacg cagccctcga tgccgccagt gattccgttc gtcctctgac
    11401 cactaatggt tgtgacgaat ctcgcctgat ggcgaactgg ctgaaaggtc aaaaagtgga
    11461 aatcgaacgc gttctggtga gcccgttcct gcgagccgag caaacactgg aagaagtagg
    11521 ggattgtctg aacctgcctt ccagtgcaga agttctgccg gagttaacgc cctgcggcga
    11581 tgttggtctg gtcagtgcct atttgcaggc gttgaccaat gaaggtgttg cttcggtact
    11641 ggtgatctct cacttaccgt tagtcgggta tctggttgcc gagttatgtc cgggcgaaac
    11701 gccgcctatg tttaccacct cggccattgc cagcgtaacg cttgatgaaa gcggaaacgg
    11761 cacattcaac tggcagatga gcccgtgtaa tctgaagatg gcaaaagcta tctgatgagt
    11821 tccaaattat gcccaatgag gtagattcat tgggcataat tgctcatcag gcagcggata
    11881 tctgtcaacc tcactactat cgaagactct ccccatctat aaataaaaca ctttctactt
    11941 tcattcggaa attattcttc tgcgcgtatc ttgattttgc ccaaagttac atgttgaaat
    12001 gaataatgtc tgtttg
//
LOCUS       DAZGOB010000142        11951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGOB010000142 DAZGOB010000000
VERSION     DAZGOB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.142"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            83..198
                     /gene="rrf"
                     /locus_tag="ACHLIF_004205"
     rRNA            83..198
                     /gene="rrf"
                     /locus_tag="ACHLIF_004205"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            521..1819
                     /gene="kgtP"
                     /locus_tag="ACHLIF_004206"
     CDS             521..1819
                     /gene="kgtP"
                     /locus_tag="ACHLIF_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO9608104.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1816..2139)
                     /gene="yfiM"
                     /locus_tag="ACHLIF_004207"
     CDS             complement(1816..2139)
                     /gene="yfiM"
                     /locus_tag="ACHLIF_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO9608105.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2185..3540)
                     /gene="pssA"
                     /locus_tag="ACHLIF_004208"
     CDS             complement(2185..3540)
                     /gene="pssA"
                     /locus_tag="ACHLIF_004208"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO9608106.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3654..6314)
                     /gene="pat"
                     /locus_tag="ACHLIF_004209"
     CDS             complement(3654..6314)
                     /gene="pat"
                     /locus_tag="ACHLIF_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO9608107.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6346..7044)
                     /gene="tapT"
                     /locus_tag="ACHLIF_004210"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6346..7044)
                     /gene="tapT"
                     /locus_tag="ACHLIF_004210"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO9608108.1"
                     /translation="MTENGVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7113..7532)
                     /gene="trxC"
                     /locus_tag="ACHLIF_004211"
     CDS             complement(7113..7532)
                     /gene="trxC"
                     /locus_tag="ACHLIF_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO9608109.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7739..8776
                     /gene="yfiF"
                     /locus_tag="ACHLIF_004212"
     CDS             7739..8776
                     /gene="yfiF"
                     /locus_tag="ACHLIF_004212"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO9608110.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8824..9513)
                     /gene="ung"
                     /locus_tag="ACHLIF_004213"
     CDS             complement(8824..9513)
                     /gene="ung"
                     /locus_tag="ACHLIF_004213"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO9608111.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9818..10201
                     /gene="grcA"
                     /locus_tag="ACHLIF_004214"
     CDS             9818..10201
                     /gene="grcA"
                     /locus_tag="ACHLIF_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO9608112.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10257..10844)
                     /gene="eamB"
                     /locus_tag="ACHLIF_004215"
     CDS             complement(10257..10844)
                     /gene="eamB"
                     /locus_tag="ACHLIF_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO9608113.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10947..11828
                     /gene="yfiE"
                     /locus_tag="ACHLIF_004216"
     CDS             10947..11828
                     /gene="yfiE"
                     /locus_tag="ACHLIF_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9608114.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNASSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2978 a         2981 c         3099 g         2893 t
ORIGIN      
        1 tgttttggcg gatgagagaa agattttcag cctgatacag attaaatcag aacgcagaag
       61 cggtctgata aaacagaatt tgcctggcgg ccgtagcgcg gtggtcccac ctgaccccat
      121 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
      181 agtagggaac tgccaggcat caaattaagc gaaaggccat ccgtcaggat ggcctttttg
      241 cgttggtgaa aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga
      301 ttcagcagcg aatacggctt ccccaacttg cccacctcca tacgtgttct cctcaccaga
      361 aatttatcct taagctcctc aataaccatt ttcctgctaa ctaaattcat ggttaaggtt
      421 gcataatgat acgcaacaaa tgtataacat tttctttacc aaaagaaata aacaaaagcg
      481 accgacaaaa gcatcggatt acggcaggag acataatggc atggctgaaa gtactgtaac
      541 ggcagacagc aaactgacaa gtagtgatac acgtcgccgc atttgggcga ttgtgggggc
      601 ctcttcaggt aatctggtcg agtggttcga tttctatgtc tactcgttct gttcactcta
      661 cttcgcccac attttcttcc cttccgggaa cacgacgact caactactac aaacagcagg
      721 tgtttttgct gcgggattcc tgatgcgccc aataggcggt tggctatttg gccgcatagc
      781 cgataaacat ggtcgcaaaa aatcgatgct gttatcggtg tgtatgatgt gtttcggatc
      841 gctggttatc gcctgcctcc caggttatga aactataggt acgtgggctc cggcattatt
      901 gcttctcgct cgtttatttc agggattatc cgttggcgga gaatatggca ccagcgccac
      961 ctatatgagt gaagttgccg ttgaagggcg caaaggtttt tacgcatcat ttcagtatgt
     1021 gacgttgatc ggcggacaac tgctagccct actggttgtc gtggttttac aacacaccat
     1081 ggaagacgct gcactcagag agtggggatg gcgtattcct ttcgcgttag gagctgtgtt
     1141 agctgttgtg gcgttgtggt tacgtcgtca attagatgaa acttcgcaac aagaaacgcg
     1201 cgctttaaaa gaagctggat ctctgaaagg attatggcgc aatcgccgtg cattcatcat
     1261 ggttctcggt tttaccgctg cgggctccct ttgtttctat accttcacca cttatatgca
     1321 gaagtatctg gtaaatactg cgggaatgca tgccaacgtg gcgagtggca ttatgactgc
     1381 cgcattgttt gtattcatgc ttattcaacc actcattggc gcgctgtcgg ataagattgg
     1441 tcgccgtacc tcaatgttat gtttcggttc gctggcagcc atttttaccg ttcctattct
     1501 ctcagcattg caaaacgttt cctcgcctta tgccgctttt ggtctggtga tgtgtgccct
     1561 gctgatagtg agtttttata catcaatcag tggaatactg aaggctgaga tgttcccggc
     1621 acaggttcgc gcattaggcg ttggcctgtc atatgcggtc gctaatgcta tatttggtgg
     1681 ttcggcggag tacgtagcgt tgtcgctgaa atcaatagga atggaaacag ccttcttctg
     1741 gtatgtgacc ttgatggccg tggtggcgtt tctggtttct ttgatgctac atcgcaaagg
     1801 gaaggggatg cgtctttagt gacgggtcag ttgccagacg gtatagccgg tgcttgcacc
     1861 ggcgacatcc caggccaaat ccttccagct ccagccgctc ccttcggggc ggctatccca
     1921 aagctctttt gacgccccca aactgacaga gaacatcaat ccaaacatgg cactgcgatc
     1981 ccggctcatc ccctgatgct gtgaatattc atttccggcg gcggaaagca tcgccgaggc
     2041 gataaagtgt tgagctttat cctgcccgct ccagctatcg ttagccatat ggctgcatcc
     2101 agaaagtaac aatagcgaac agacaaaaag aatacgcaaa acgtggcctc caaaaaacaa
     2161 accccgtaca ggacggggtt gtgattacag gatgcggcta attaatcggt cgatgcggat
     2221 acggcgcaaa cggcggatga gtttacgaac cttcaccgga taatcggcaa tactttgcag
     2281 atcgcgatag tgcttaacga tggtggtatg ctcgcggatc agctccagtt ctttctctcg
     2341 ctgcggcgca agctccagtt gtggatcgtg gatcaaaatg gcgttttcca gatccagacg
     2401 ccaggcgcgc gggttcaggt tattaccggt gatcaacatc cacttatcat caacccacat
     2461 cccttttagg tgataggtgt tgtcgtcatc tttccataac cgaaccacta gctggtcagt
     2521 attgacgtaa tactgcaaac ggctcaggaa acgacgcaga ttgatctcat agagataagg
     2581 caatgcgcca attatcttga aaggttcatc ttccggaatg tagaagtcgt tcgcggtttt
     2641 atcaccaaca ataatttcga cctttttccc ttcgcgcagc aactggataa tattgcgcac
     2701 aaggattgct ggcaggttga agtatggcgt acagatggtt agtttctgct cggcacaagg
     2761 cataagatgg aaaatggtct tgttcaacag actcgatttc cccagcccca ctagcggcgt
     2821 tacggaaagc tgatcgttgt cggcatcgcc ctggaaatga taagcggcat cacgcagctc
     2881 ctggcggaac agacgaatat cgttcttgat ttccgggctt tttggccgat taacatcatc
     2941 cagacgatta acgccgcggc cattcataat attctgtgta acccattcaa acataatgtc
     3001 tgacatctta cggttacgga taagatgata acggtcgtag cgatatttat cgtgctgatg
     3061 caggtaaaca tcgttcaggc tggcaccgct ataaagtacg ctatcgtcga tgataaagcc
     3121 tttaaagtgc agaacaccaa gggcttcacg ggtattgatt ggaacgccat aaaccggaac
     3181 atctacgccc ggattttcct gcgccatgcg gcagtaccag tcagcgttag tgttagatgc
     3241 cgcagcgcca atgcgtccac gttgtgcacg atgccagtcg accagcaccc gcacatccag
     3301 ttccggacgc tgccttttag cttcatacaa cgcgttcaga atgcctttgc caccgtcatc
     3361 ctgttcgaga tacagggcga caatgcaaat gcgctgcttc gcgctggcta ttttttccag
     3421 cagcgtctcc cggaagtcgg cgggagcgta aaagaaatcg acatcatcaa ctgattgaga
     3481 aatcttgggt agttgggcaa ggtgttgttg atgtttatta cgcttaaatt ttgacaacat
     3541 cacagtgcat ttcttctctg ttcattgaag ggtcctctgt gcaatgcaga cgacataagc
     3601 gggcaataat aacaccagtc ccgattaagt ggtcaacatt tccagtacct tactcatgat
     3661 tcctcgcgtt gggcaagatt tagcgtaagc ccaacgatcc cctcttcgag ctggatatca
     3721 acgttaaacc cgagcttgcg ggctagcgcc accatgccac gattgtttgg catcgtaata
     3781 ccattcagac gttgtagtcc gtgatctcgc gtataggtaa tcaacttttc cattaagcgt
     3841 cgacctaagc ctaacccttt gagatccgag cgaaccagca cagcaaattc ggcatcgatg
     3901 ttatcaggat cggaaatcgc acgcgtgacg ccgaggatct cttccgtttg atcaatacgt
     3961 cgtaccgcta caaacgccat ttcccgatcg tagtcgatct gtgtcatgtt ggctaaatct
     4021 tcatgggtaa attcgttgat ctcgctaaag tagcggtaat aaagatcttc tttggtgact
     4081 cgcgaaatga attgctgaag ttgtggctca tcttctggca aaatcgggcg gaacaagcag
     4141 cgttcaccgt ttttcaattc tacccattct tccagctgat gcggataagg gcgcactgcc
     4201 agccgactct cgttatcgcc ttcaaacggc gagatatcca gcgtgacatc cagcgcggta
     4261 aattcactgc cagaagccag caaaggatga atatccagac gctgaatttc cgggcaatcg
     4321 acaatcaagt tggaaacctg caccagaagc tggctcaagc ctgcaacatc caatgggcgt
     4381 agcgcactgc gtgcacgaat ctttttactt ttgatcccct gaataaccag atagcgggcc
     4441 aggttcatgt tcagcggcgg taacgcgacg acggcttgat cttcaggacg ccactccaca
     4501 ccgccttcgc ccagcatgat caacggcccg aaaaccggat cgtgctcaac cacaacccgc
     4561 aactcctgag caccagcacg gttagccata ctttgcacca acaggccgtg gacccgtgcc
     4621 tgcggccagg ccatttttac gcgatcgaaa atggcgtttg ccgcttgctg gacttcattg
     4681 gctgtacgca ggtagagcat gacgccctga acttccgatt tatgtggaat atccggcgaa
     4741 cgcaatttca gcgccaccgg ataaccaatc tgttcggcaa tatgcaccgc ttcggtgcta
     4801 tcgctggcaa tccaggtggg gagcgtgttc atgccatatg cttgcaggat aggctgaact
     4861 tcatgggtat cgagtgacgt tgctccttcg gcaatcgcct gttgcaacag aagatgcgct
     4921 tctgcggtat tggaggtcag attgctgggc aacgccggcg tttcgcgtag ttgcttctga
     4981 ttacgccggt actccaccat atgcataaaa gcagtgatgg ttccttccgg ggtacggtag
     5041 gtcggcagcc cggcttcgct gaataatcgt cgtgcctctt gcgaggaatg ttcgccacac
     5101 cagttcgtca gcagagaaac atacttgctg cggggatgat gctttaccgc ttcaattaat
     5161 acttgcgcgc tttctgttgc gggagcagcg gcgctgggcg aatgaataac catcagcgca
     5221 tcaaaatcct ggctgtggag cagaatgtcc agcgttttaa tatagtgctc actgctggcg
     5281 tcatcgcgca gatcgagcgg gttagagatt gccacatgtc ctggcagtgc atcgcgcagt
     5341 ttctggcagg tttcttcgct tagtgttgcc agcttgccat tgcgtgacca taaggcatca
     5401 agcgccagcg cggcaggcgc agcaccgttg ctgataatca tcaaccggtc gccacgcagc
     5461 gggcgcatat ggctaagggt ttccaccgcc gaaaacagtt cgtgggtgtc ctgtacccgc
     5521 aacaaaccgg cacgctgaat agccgcatcc cacgccggat ccattcctgc cgtggtgttc
     5581 aacagacgtt gagccgccgg gctacgtccg cttttaatca ccagaatcgg tttgttgcga
     5641 gaggcactac gggccgccga aacaaagcgt cgcgcgtcgc ttaactgttc gagatagagc
     5701 aggatggcgc tggttttact gtcgcgtgcc agatagtcaa gcaattcatc aacgtcgata
     5761 tccaggctgt cgccgagcgc aataaagtag gaaaagccca tcttacgctg ttgcgcccag
     5821 tcgaggatgg tgttggagac ggcagccgat tgcgaaataa acgccagctt gccgcgttta
     5881 atcggcacag gcgaaaagct ggcattcaga ccttgccagg gagccagtaa acccagactg
     5941 tttggtccaa gcaggcgcat gttatggcgc agggcgcagg cgcggagatc ttcgtgttgc
     6001 gatgccgggg cggaaagaat aatgcaggtt ttacagcctt tctcgccgag ctcttccaga
     6061 agagcaagat tacggctggc attggtacat aaaaccgcaa ggtcgggtgt aaagggcaag
     6121 ctggcaatat ccggccaggc caacacaccc aacaccgctt tccaggctgg cgtcaccggg
     6181 agtaccggtc cgttaaagcc tcccgccagc aggttacgca tcatcaggta acctgcgcga
     6241 ttgggtttca tcgacgcgcc aattaccgct atcgattttg gtcgcagtag tgcttccagt
     6301 cctcgctgac tcataccggt ctcccgcaga agtgaccgaa tgattttaaa cgctttctaa
     6361 ctgttctgct gtgatgctac ccagatgttg cgtttttcct gccagatagc gtgttttaaa
     6421 gcgggtaaaa tgctcgccta accctgctgc cgccccggta tcgctggcca tatctaacag
     6481 tgcgatggct acctcggcag tacaatattg gcctgcagcc tgggcttcac gcaggcgata
     6541 ggcagaaagc cgggaaagat cgacggaaat gacgggaaga ttatccagat acggactttt
     6601 acgaaacatc ttgcgagctt ccggccaggt accatcgagc atgataaaca gcggtggctt
     6661 accggcaggt ggtgtgaaga tcacttcccg ttgctcatca gcatacgaag cgggaaagac
     6721 caccattggc tgatagtccg ggttttgcac caactccagc aaatcctgcg agggttcggt
     6781 acgcgaccat tgaaacgcaa cggtatcagg caaaatatca gcaatcagac gcccggtatt
     6841 actgggcttc attggctcgg tgtcgaacat cagcaaacag aagcgacttt ttgcttgtgc
     6901 tggggtaatt gtcgaacaga gacataattt ctctggcaaa agacagcgtt ggcagcgacg
     6961 aacgcgatta ccgcgggcaa gaaaaggacg tgttgcgcgc gcaatacgct cggcgcgtaa
     7021 ctggagaaca ccgttttcgg tcataagaga gcgtcgaaaa aacgccattg tcgcagagga
     7081 gaaaacgggg cacaagatgc gccccggtaa gattaaagag attcgttcag ccagctatcg
     7141 aacggcgctt tcggtactgc gccattaagc atgtcgacaa cctgaccgtt tttgaaaatc
     7201 atgatcgtcg gtatactacg aattccaaag cgactgctca attcacgttc agcttcggta
     7261 ttcactttca caaagcgcac tttaccgcta cgctcttgcg cgacatcttc aaaaattggt
     7321 gcgaaattac ggcaggggcc gcaccacggt gcccagaagt cgatcaccac aggtagatca
     7381 tccttcagca atttgtcgag tgtttcaccg gtcgcattaa tcacctctcc gtcaaacaag
     7441 tcgtgaccgc agcgtccgca ttttgccgca tcttcgatcc gatcgtcggg aatgcgattg
     7501 atggcctgac aatgggtaca aacggtattc ataactaacc tcgggatgag taaggagact
     7561 tatcggcaac gacgccggta tgtttctaat atgttacata ttatcgggga cattgttcca
     7621 gacaacaatc tgttttattc aataggtatg atagtcgcag gctttggtac gaaatcatgg
     7681 cgaaccgcgc ggacttcggg taatctgcgc gcttcgcgca gcgctggtgg agaaaagcat
     7741 gaacgatgaa atgaaaggta aaagcggcaa ggtcaaagtg atgtatgtcc gcagtgatga
     7801 tgattctgat aaacgtaccc acaacccgcg taccgggaaa gggggcgggc gtccaggaaa
     7861 atctcgtgct gacggtggcc gtcgccccgc ccgcgatgac aaacagagtc agccccgtga
     7921 ccgcaagtgg gaagattcgc cgtggcgcac ggtttcccgc gcgccgggtg atgagacgcc
     7981 ggaaaaggcc gatcacggtg gcatcagtgg taaaagtttt attgatccgg aagtgttgcg
     8041 tcgtcagcgt gcggaagaaa cccgcgtcta cggcgaaaat gcatgtcagg cacttttcca
     8101 gagccgtccg gaagcgattg ttcgcgcctg gtttatccag agtgtaacgc cgcgttttaa
     8161 agaagccttg cgctggatgg cagcaaaccg caaagcgtac catgtggtgg atgaagcgga
     8221 attgacaaaa gcgtcaggca cggaacatca cggcggcgtt tgcttcttga tcaaaaagcg
     8281 taacggtaca accgtgcagc agtgggtaag ccaggcaggc gcgcaggatt gcgttctggc
     8341 actggaaaac gaatctaacc cgcataacct gggtggcatg atgcgcagct gcgcgcactt
     8401 cggcgtgaaa ggtgtcgtgg tgcaggatgc ggcactgctg gagtcggggg cggctatccg
     8461 taccgcagaa ggcggcgcag agcacgttca gccgattact ggcgacaaca ttgttaacgt
     8521 gctggatgat ttccgtcagg caggttacac cgtagtgacc acttccagcg agcagggtaa
     8581 accgctgttc aaaactagtc tgccagcgaa aatggtactg gtgctgggtc aggaatatga
     8641 agggttaccg gatgccgcac gcgatccgaa cgatctgcgc gtgaagattg acggtacggg
     8701 caacgttgcc gggctgaata tctctgtcgc aaccggcgtt ttgcttggtg aatggtggcg
     8761 tcagaataaa gcctgataaa tcagccgggt ggcaactctg ccatccggca tttccccgca
     8821 aatttactca ctctctgccg gtaatactgg catccagtca atcggcgtct cgccacgttg
     8881 ttccagccac tgatttgcca gcacaaaatg gttgcagcca aagaatccac gatgcgccga
     8941 aagcggcgac ggatgcggtg ctttcagtac atgatggcgt tgcttatcta taatcgcccc
     9001 tttcttttgc gcatgtgatc cccacaacaa aaacaccacg ccttcgcggt gctggttaat
     9061 caggctgatg actttatcag taaacgtctc ccaaccgagg ctggcgtggg aatgcgcctg
     9121 acctgcgcga accgtcaaca cagtattgag cagcagaacg ccctgacgcg cccagctttc
     9181 gagatagcca tgattagggc gggtgaagcc cggaatagta ttttccagct ctttatacat
     9241 attcaataat gacggaggaa tggcaatgcc gggacgaacg gaaaatgcca gaccatgcgc
     9301 ctgtcccggt ccgtgataag gatcctggcc gagaatcacc actttaacgt cacccaactc
     9361 ggtaaagcgg aacgcgttaa agacatcttt ttgtggtgga tagatagtga cgccggactg
     9421 ccgctcgctg gcgacggtct gaagggtatt aagaaaataa ggttgttgct tctcttcagc
     9481 cagcacgtca tgccaggtta attcgttagc catctcgctc tcctgcgaat cttcaatccg
     9541 cctagcttaa ctgcttcttt ctacagaaca aaatcatgct cattttttag acttaaacga
     9601 gtgcaaaaaa atgtaaattg gtaaaatttc acacccgcat aagttgatgt aaaacaataa
     9661 aatccattta cgaccacaat cgatgtgtgg tttttattga tttaaatcaa agaatcaagg
     9721 gtggttgagg agtatatata cactcaagca acaatggttt taccaattgg ccgcgacagg
     9781 ctgaacaaat caaataattt tgccggggag gcatcacatg attacaggta tccagattac
     9841 taaagccgct aacgacgatc tgctgaactc tttctggctg ctggacagcg aaaaaggcga
     9901 agcacgttgc atcgttgcaa aagcaggtta tgcagaagat gaagtggttg cagtaagcaa
     9961 actgggtgac attgaatacc gtgaagttcc agtagaagtg aaaccagaag ttcgcgttga
    10021 aggtggtcaa cacctgaacg ttaacgttct gcgtcgtgaa actctggaag atgcagttaa
    10081 gcatccggaa aaatatccgc agctgaccat ccgtgtatcc ggttatgcag ttcgctttaa
    10141 ctctctgact ccggaacagc agcgcgacgt tatcgctcgt acctttactg aaagcctgta
    10201 aggcttaagt agcaagttac taagcggaag agggcgacct cttccgcttt ttttcgttaa
    10261 tagaaaatgc gtaccgcgca atagaccagc aacagggcaa gcacgatatt taactggcga
    10321 ccatactggc gaaacaatcg ctgaaacaga tgccccgcca gcgcccagca cacattgcca
    10381 aacgtcccaa tcatcgccag caaaacgctg acgccaacta cccagcttag cgcctgtgtt
    10441 tgcggcagaa caaacgtcga cagtgccgta acaccgtaca aaatgatttt gacgttcaca
    10501 aactgcaaag caaagctggc ccaaaagctg attggttttg cctgaagtcc gtcttccttt
    10561 gttgggctgg tggcgatttt ccacgccagc cagacaatat atgccgcccc cgcccaactc
    10621 aaaaggtgta ccgctgccgg gtcaatcact gccagtgaaa atgaaatgcc cgcacacagt
    10681 aacatcacaa tcaaaaatcc cagactcatc cctgccagca cgcgggtact ttgacgaaat
    10741 ccatgcgacg tagcagagct aagggcgaga atattgttcg gtcctggcgt catagcggta
    10801 atcagggtgt aagtccaaaa agcacttaaa agggtcggtg tcacttgtta tctcctctgt
    10861 tttggtagac agtacccgac acggaacatt taaaaaaatg aatgttttta tattctagat
    10921 tcgatatttt cgataggttt ggggttatgg atctgcgccg ttttattacg cttaaaaccg
    10981 tggtggaaga gggttccttt ttgcgagctt cgcaaaaatt gtgctgtaca caatcgacgg
    11041 tgacttttca tattcagcag cttgagcagg agttttcagt ccagttattt gagaaaattg
    11101 gccgacggat gtgccttacc cgcgaaggaa aaaagttgct gccgcacatt tatgaactta
    11161 cccgagtgat ggatacgctt cgtgaggctg ccaaaaagga gtcagatccg gacggggaac
    11221 tacgcgttgt ttcgggcgaa acgttgctct cttaccgaat gccgcaggtg ttgcagcgtt
    11281 ttcggcaacg cgcaccaaaa gtgcgtttat cgttgcagtc gctgaactgt tacgtgatcc
    11341 gtgatgcgct actgaatgat gaggccgatg tcggcgtctt ttatcgcgta gggaatgatg
    11401 atgccctgaa ccgacgagag ttgggtgaac aatcgctggt gctggtggct tcaccgcaaa
    11461 ttgcagatgt cgattttact gagccgggaa gacataacgc cagtagcttt attatcaacg
    11521 aaccgcaatg tgtcttccgg cagatatttg agagcacgct gcgtcagcgg cggatcacgg
    11581 tggaaaacac cattgagctg ttaagcattg aaagtatcaa acgttgtgtc gcggcgaata
    11641 tcggtgtcag ttacttgccc cgctttgcgg tggaaaaaga gcttgagagc ggagaattaa
    11701 ttgaattgcc ttttggcgaa cagtcgcaga ccattacggc catgtgcgca caccatgctg
    11761 gaaaagcggt tagcccggcg atgcacactt ttattcagtg tgttgaagag agttttgtgg
    11821 ctggataaaa ataatgtcgg gattaaaccc ggcatttaac ttgtcgacaa agtgcgcttt
    11881 attcatgccg gatgcggcgt gaacgcctta tccggcctac aagatcgtgc aaattcaata
    11941 tattgcatga g
//
LOCUS       DAZGOB010000143        11588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGOB010000143 DAZGOB010000000
VERSION     DAZGOB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.143"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..225
                     /locus_tag="ACHLIF_004217"
     CDS             <1..225
                     /locus_tag="ACHLIF_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608115.1"
                     /translation="DRFNSLKQEFSGVPDDAADALSSMPELIRAAFFLLSTREYKSTG
                     LDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            218..451
                     /locus_tag="ACHLIF_004218"
     CDS             218..451
                     /locus_tag="ACHLIF_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608116.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLDPVIMELQEIDILHDRYKEQHTGE"
     gene            457..756
                     /locus_tag="ACHLIF_004219"
     CDS             457..756
                     /locus_tag="ACHLIF_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9608117.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVTIKGVEERRITY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            753..2153
                     /locus_tag="ACHLIF_004220"
     CDS             753..2153
                     /locus_tag="ACHLIF_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO9608118.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            2354..2605
                     /locus_tag="ACHLIF_004221"
     CDS             2354..2605
                     /locus_tag="ACHLIF_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608119.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2602..2907
                     /locus_tag="ACHLIF_004222"
     CDS             2602..2907
                     /locus_tag="ACHLIF_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608120.1"
                     /translation="MSEPKFGEKLYKHNGLITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2917..3327
                     /locus_tag="ACHLIF_004223"
     CDS             2917..3327
                     /locus_tag="ACHLIF_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9608121.1"
                     /translation="MTAQIAAYGRLVDDPLVKQSSKGTPMTLARMAVSLPCSQAHDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGDDDPYGDDIPF"
     gene            3338..3610
                     /locus_tag="ACHLIF_004224"
     CDS             3338..3610
                     /locus_tag="ACHLIF_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9608122.1"
                     /translation="MVHDRIAEELEAKGFYRRASARWGEVMLLVETDKERHQVTMRRL
                     ECSRKAQRPPEPPDNYGDLKKAVDRTYAEMGMDGAGDEIWRNYQDS"
     gene            3567..3695
                     /locus_tag="ACHLIF_004225"
     CDS             3567..3695
                     /locus_tag="ACHLIF_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO9608123.1"
                     /translation="MVLVMKYGEITRTANHTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            3736..3960
                     /locus_tag="ACHLIF_004226"
     CDS             3736..3960
                     /locus_tag="ACHLIF_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608124.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
     gene            4252..5409
                     /locus_tag="ACHLIF_004227"
     CDS             4252..5409
                     /locus_tag="ACHLIF_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO9608125.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGATFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            5449..6021
                     /locus_tag="ACHLIF_004228"
     CDS             5449..6021
                     /locus_tag="ACHLIF_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO9608126.1"
                     /translation="MGEKIMKSMEIRSSEITTSGSNTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            6023..7234
                     /locus_tag="ACHLIF_004229"
     CDS             6023..7234
                     /locus_tag="ACHLIF_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9608127.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNMEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            7231..7569
                     /locus_tag="ACHLIF_004230"
     CDS             7231..7569
                     /locus_tag="ACHLIF_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO9608128.1"
                     /translation="MNIGRLRDRVTIQTLKQTRAMTGEILETWLDGHTLWASVNMISS
                     KEAISSGAELATGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7566..7859
                     /locus_tag="ACHLIF_004231"
     CDS             7566..7859
                     /locus_tag="ACHLIF_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO9608129.1"
                     /translation="MTELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRRF
                     DDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            7859..8299
                     /locus_tag="ACHLIF_004232"
     CDS             7859..8299
                     /locus_tag="ACHLIF_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO9608130.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPIDGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREDEERAANRNNWMYDADE"
     gene            8283..8465
                     /locus_tag="ACHLIF_004233"
     CDS             8283..8465
                     /locus_tag="ACHLIF_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608131.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8588..8944
                     /locus_tag="ACHLIF_004234"
     CDS             8588..8944
                     /locus_tag="ACHLIF_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO9608132.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            8928..10589
                     /locus_tag="ACHLIF_004235"
     CDS             8928..10589
                     /locus_tag="ACHLIF_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO9608133.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10603..10884
                     /locus_tag="ACHLIF_004236"
     CDS             10603..10884
                     /locus_tag="ACHLIF_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608134.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRALELLAYRRRVEMYREKEKITYHWKKPERLRR"
BASE COUNT         3137 a         2737 c         3275 g         2439 t
ORIGIN      
        1 gatcgtttta attccctgaa acaggaattt tccggcgttc ctgatgatgc ggctgatgcg
       61 ctttcgtcaa tgccagaact tattagagcg gcttttttct tactttccac gagagaatat
      121 aaatcaacgg ggcttgatgt actgaatatc gccgccgatt atgcggaata tgtggcagag
      181 gcgcgttata gaagaaaatt tcctgaggat gtaagccatg cgtgatattt accaccaaac
      241 aatagaccgc gcatttcttg cactttctca cagtgaaaac atgctggaaa tattgcgcat
      301 atggcttgaa acacttggcg acaatgaacg cgacaaacaa aaatcaagaa ttgccacggc
      361 attaataacg cttcttgatc ctgtaataat ggaactgcaa gaaatagata tattgcacga
      421 cagatataaa gaacagcaca ccggagaata aaaataatga aacttaaata ttctggctta
      481 actgccagtg gcaacactca ccctaaattt acgcgcggcg atatttaccg cgaccagtac
      541 ggcggcaccg taacgattaa aggtgtagaa gagcgacgca ttacctaccg ccgtgaaggc
      601 tacgaatatg actgcgtgat gcctgtttat cagttccggc gtgatttttc tctggtacag
      661 gcagcgcccc gcagtaaacc aaccagcagg gagaaagcac gcgccaatat tcaggaaata
      721 aaaaagatgc ttaacgtatt caggggcaag aaatgaaaag cgcaccgaac ttaaaaaaac
      781 agccttacga caagatgacc gaagtcatca tttttgcggg tagtgatgcg tgggcacatg
      841 cgaaacagtg gcaggaacag gacgggcgac tggctggcga taatgtgcct cccgttgtgc
      901 tggctgatga tcaactggat gaactggcag acctgagaat catcgacgag gggcgctatt
      961 gtgtccggct gtacaaggca ggccacatca ggccatcaaa tattaatgcc attgcgcaca
     1021 agctggcggc ggcgggtgta actgatgcga attattaccc cgaagggatg cacagccata
     1081 tgcgggagaa ctggcgcgaa tacctggaac gggtgcgtgg gaaagagccg gcggaagaaa
     1141 aaaaccacca gcgaaaaacc acgctaccga tgagcgttgg atctaccgga tacgacacgc
     1201 aactggatta cgtggttaag gggattattc cggcggtatc gctatgcagc atatacgggg
     1261 ctagcgggtc ctataaatca ttccttgccg gatcgtgggc gtgccatgtt gccactggtc
     1321 gccagtgggg aggccgcagg gttgcgcatg gtgcggttct ctatgtggtt ggtgaaggcg
     1381 gtataggtgt tccgcgtcgt gtaaaagcct gggaggttgt gcacgatgag caggtgaaaa
     1441 atctgtatct ggtaaaccgc cccatctttc cggctgcccc gcttgatgtt gatgaaatgg
     1501 ttatcgctgc ccgtcaggtg gagcgggaaa cgggtaaacc tgtacgcatg attattctgg
     1561 atacgctggc gcgttgcttt ggtgggaatg atgaaaatga ttcccgtgat atgggggcgt
     1621 ttatccgtgg ttgtgacgaa ctgaaacgac gcacaggggc cacggtgctg gtggttcacc
     1681 attccggcaa ggatgagacg aaaggcgcgc gcggttccag tgcatttcgt gcttcgctgg
     1741 atgctgaata ccggatacgc agggaggacg caggaagcga agcgctggtt atctcatgca
     1801 ccaaaatgaa ggacgcggag gaactcaaag aagccgcata tgacttacgc gtggtggagc
     1861 tttttaccga cgctgacggt gaattaatca cgtcgctggt ggtggtggat gatccgcgcc
     1921 ctcctgttga actggagcgc atcgaggagg cagggaacaa gacggaaaac cataccgcgc
     1981 tatgggggtg catccgttca cgcacacaga acggcgacaa gtgcacgatc ccgctgttac
     2041 gtgatgacat gaaaaagctg gggtatgaaa tgaaaaactt ccggcgctgg ctgtacaagc
     2101 tggaaaaaga tggggttatt cgtatcgatg gggatgatgt agcgccgcta taaaagtgag
     2161 gagcaaaagc gaggtggata gaaagagggc caaaattagc ctgctctccc tcacttttcg
     2221 acctgtatac atcctcaaaa gtgaggggta aaaaaatact tatgaaacac acacatagaa
     2281 aaaccgaaaa tcccagctgc gacgaagtga gacgcttgaa aaagtgaggc gaaaaagtga
     2341 gaggttgcga gaaatgaccc aaaaacgcag agacagaaca gagccaaaat ataaagcgtt
     2401 agacatgact gagcacacct taaaggtggc aatcagaacg atagaccgcc acacgcggga
     2461 aggatacgcg aaggaacatc ccgacctgat aagcgcattc atgaccacgg cggcggcaaa
     2521 ctttgccacg ctgacagaac gggagattgc cgaagcggaa caggtaacaa ccatcaacgt
     2581 taaaaccgga gaggtggaat catgagcgaa ccaaaatttg gtgaaaaact ttacaagcac
     2641 aacggactga tcacgattct gcaaataagc gcagctacac caggctggtg ggttgaaaca
     2701 gacgaaggat cctcacccgt ggcatcatgg gcgctttgtg tgcttagtta ccctgaccgc
     2761 gatgtgtatc aggatattct gcctgtgata tccactgaca aaggcatgaa acccgtagac
     2821 atcaaaaaca tgggttttca gtgcgtaatg ctcactgaaa aaatgatgga agagatgaaa
     2881 aagaacaacg ccggatcgct tcactgaggt ggcaatatga cagcacaaat tgcagcttac
     2941 gggcgcctgg tggacgaccc gctggtaaaa cagagcagca agggcacacc gatgactctg
     3001 gcgcgtatgg cggtatcgtt gccatgcagc caggcacatg acggacaggc gacgttatgg
     3061 ttatcggtta tcgcatttgg caaacaggcc gatttcctgg ctaaacatca aaaaggcgat
     3121 gttgccagcg tatccggcac gatgcaggta agccagtgga ccggacagaa cggggaaaca
     3181 cggcaggggt atcaggttat tgcagacagc gtaatcagtg cccgtgcggc acgtcctggc
     3241 gggaacagac gcaaaaccac aggcacacag ggtaatcagc caccagcggg agacgatgac
     3301 ccctacggtg atgatattcc gttctgaggg ggtgacgatg gtacatgacc gcatagcgga
     3361 ggaactcgag gcaaaaggct tttaccggag ggcgtcggcg cgatggggtg aagtcatgct
     3421 gctggtggag acagacaagg aacggcatca ggttacgatg cgacggctgg aatgttccag
     3481 gaaggcacag aggccaccgg agccgccgga taattacgga gacctgaaaa aggcggtcga
     3541 tcgcacttat gccgaaatgg gtatggatgg tgctggtgat gaaatatggc gaaattacca
     3601 ggacagctaa tcacacagcc ggagaaatcc ggcttttttt gcaccagttg aaacggtatg
     3661 gcgcattacc gggttttcgt cacggtcagg catagttact atctgaaaca aacagacaca
     3721 acagaggaaa aaacaatgcc gatgaaattt gatgagatat taaaacagcg tgataaatac
     3781 catgctgaca acatggagac gatgagcatc aatgattacc gcgcattcct ggagacgggc
     3841 gcactgattg aaaaggatca gcatggtttt gtgagatgtg ctctgtccgg tgaaatgctg
     3901 gcggtaaatc ctgaacagat agatgcattg atagaatttc tgaaagggat cagggactga
     3961 gcacgcatac agccggagca atccggcttt ttgtcatttt ttgtaaatta tttgttcgcg
     4021 gttgttccac gttgttcact gacaggatcg gcatatttta cccgaactga atcatgatta
     4081 ttctcgcccg tggtgccagg acgctggggc cacttttccg cctgttaatg ttctcgccaa
     4141 tattcattac caggcgggaa aacgatcggt gcgattgctg atttccttat gaaaaacggt
     4201 tgagtttttg ccgcgtcctg gagttcctta cttaacccca ggactttttt tatgccgaga
     4261 ataatcgaat tacgccagca gaaaaccgcc attaaaaatc agatgcgcga catgctggag
     4321 aacgcggaaa aagaaaaccg cagtcttaac gatgctgagg gcgcaacatt tgacgaatta
     4381 cgcgctaaag ctgaatccct cgataaagac atttcccgcc ttgaagccat tgcagacgaa
     4441 gagcgcagca agccaggtaa aagcagtcag accactgacc ccgcagaact ccgcaactac
     4501 attctgacag gtgaaacccg cgcattaagt acaggcgttc ccgctgatgg tggttatacc
     4561 gttatccctg aactgaacac cgaaatcatg cgaatgctgg cggatgaatc caccatgcgc
     4621 cgcatctgta ccgtgaagaa aatcagcagc aacgagttta agcagcttgt ttccgctggc
     4681 ggtgcgaccg ttaaccacgg tgaagagggt aaggcacgcg aacagaccag caccccgcag
     4741 attaacgagg tgagcattaa gctgtatccg gtctatgcgt acccgcgcac cacacaggaa
     4801 atcgtggatt tttccgatgt ggacatcctt tcatggctga cgggtgagat tggcgacacc
     4861 ttcacggaaa ccgaagaaag cgatctggtt gtgggcgatg gtgacaaaaa agcaaaaggc
     4921 tttttatccg taccccgtgc agagaagaac gacaaagagc gtgattttgg tacgttacag
     4981 gtaattaaac cttccgaatc tctggcgtgg acatctgcgg acccgctgat cgacctgaaa
     5041 tttgcattac gtaaaaaata ccgcaaaaac gcggtctggg tggttaactc cacgacggcg
     5101 gcaaaactcc agaaggtgaa gaacgcgaac ggtgattata tctggcgcga ccgtttacag
     5161 gcgggtgatc ctgatacgtt gctgggcctt ccggtcgaat atctggagtt tatgcctgat
     5221 aacgttattg ccctgggtga cttcaaacgc ggttactaca ttgttgatca cgaaacaggt
     5281 gttcgcacca gaccggacaa cctcacagag ccgggcttca tcaaaatttt cacgcagaaa
     5341 tatttaggcg gtggcgtggt ggattcgaac gcgatcaaga ttctggaact gccacaggac
     5401 gacgattaac agcatacaga aggggcttaa aagccccttt agtgttttat gggtgaaaaa
     5461 attatgaaga gtatggaaat ccggtcatcg gaaatcacca ccagcggcag caacacgctt
     5521 acaggctacg ttgttcgctg ggataagctt tcagaactgt tatggggtga gttttacgaa
     5581 aaattccagc ggggggcgtt tactgaatgg cttgcggcgg gtaatgacgt tcgcggcctg
     5641 tatgagcatg accacagtat gttactgggg cgcacccgtt ccggcacgct gaaactggaa
     5701 gaggatgaaa cagggttacg ctttgaactg accccaccgg ataccagtac agggcgcgac
     5761 gttatcgaac tggttaagcg tggtgatata tccggcatga gctttggctt tcgttcccgt
     5821 aaggatgtat gggataccac aacagatcca tgcgtgcgca ccgtgctggt tgcggaactg
     5881 tacgaaatta ccgtcacatc cgtaccggct taccctgatt ccggcgtgga gctggcccgc
     5941 cgctccctgt acgagcagca ccccgaaaaa atgccgcgtg cggataatcg ccgctggtgg
     6001 gcggatttag cgggggtgtg atatgtggcc tttcagaaga aaaaaagagc agcgcagcat
     6061 gacgcttgat gagtttatgg cgctggctgg cacatcgaac acgggggcgg gtgagtacgt
     6121 atcatcgggg acagcggaat cactgcccgc cgtcatgaac gccgtgacgg tcatctctga
     6181 ggcggtggct accatgccgt gttacctgta cctggtacgc aatgagaagg ggaaggaggc
     6241 ccgcgagtgg cttgattctc atccggtcga tcacatcctc aacgagcgcc cgaacgcgtg
     6301 gcagactccc taccagttta agcgaatgat gatccgccac tgcctgttaa acggtaatgc
     6361 ttacgcggtg attcagtggg ggcgtgatgg ttttccggcg gctttgcatc cttacccgcc
     6421 gcagtcggtg aacatggagc agacaggaga acacaactgg cgctactgca tcactgacgc
     6481 ttacaccgga aacacccgca actatttacc gtgggaggtt ctccaccttc gttactccac
     6541 ggatgacggt tttatggggc gctcacctgt aaccatctgc cgcgaatcgc tggggcttgg
     6601 gctggcccaa caacgccacg gcgcgagcgt gatgcgtgat ggcatgatgg cggcaggggt
     6661 tatcacgtca ggcgaatggc tggacggcgt gaagggcaaa caggcattag ccgcactgga
     6721 acgctacaaa ggggccagaa acgccggaaa aacgcccatc cttgaagggg gtatgagcta
     6781 tcagcagctg ggcatgagta atcaggatgc tgaatggctg gcctcccgtc gcttcaccat
     6841 tgaagacatc gccagaatgt tcaacgtctc gccgattttt ttgcaggaat acagcaacag
     6901 cacctacagc aatttcagcg aggcaagccg cgcatttctc accatgacga tgcgcccgtg
     6961 gctggcgaac tttgagcagc agataaaaaa cgccctgctg gtggcctcgc ctgtacctgg
     7021 tatccggtat caggtggagt ttgacagcgc ggacctgtta cgggccacac ctggcgaacg
     7081 ctttgccacc tatgagcgcg gcatcaaatc cggcgttatg tgtccgaacg aagcccgcga
     7141 acgtgaagga ctgtcaccgc gtgatggtgg tgatgagttc agccaggcat ggaaacagga
     7201 agtaaaaatc agcgagggag aaaaaccgga atgaacatag ggcgactgcg tgacagggta
     7261 acgattcaga ccctgaaaca gaccagagcc atgacgggtg aaatactcga aacgtggctg
     7321 gacggtcaca cactctgggc aagcgtgaac atgatcagca gcaaggaggc catttcatca
     7381 ggtgcagagc tggcgactgg aactgtaagg atatggatac gctaccggaa ggacatcaac
     7441 gccaccagtc ggataaaggt caatacgggg ccgctggcgg ggcgtgtact gaatatcatc
     7501 gggcaaccgc tgccagatgc cgccaggaca cgccttgaaa ttctttgtcg tgagggcgcg
     7561 gaaaaatgac agaacttatc accctggaag aagtgaaact ccattgccgc atcgatggcg
     7621 acgatgaaga ccagttaatc agcggataca ttgccgcatc gcttgaggcg tgccagatac
     7681 acataggcag gcgctttgat gacgggctgg agttcacgcc agccataaag attggctgca
     7741 tgatgtttat cgctcacctg tatgaaaatc gccagattgt cgcggataac gcaaaaacgc
     7801 gcgtacccat gacgataggc gcactctgga cggcttaccg tgatgtgggg gtgtactgat
     7861 gccgtggcaa ccattaagac gatgtacaga accaggctgt aacaggcgcg ttaagtccgg
     7921 taagtgtgag gagcacaggc gggcggcatg gcgtgcagag gatgccagac gaggacaccg
     7981 ccgcgcgcgt gggtactcca gacagtggga caaataccgc gccatgtatc tgagtaaaaa
     8041 cccgttatgc gtgcgatgcc ttgagaaggg gatatatacg cccgccgtgg tggtggatca
     8101 cattatcccc atcgatggcg gcgatgatgt tctcttctgg cccgaatgga accatcagcc
     8161 attgtgtcag gcgtgtcata accagaaaac gaaatggctt gatccggcaa caaaaagcaa
     8221 gcgtgccgca ggtggatttc gtgaagatga agaacgggcc gctaaccgca ataactggat
     8281 gtatgacgct gatgaatgag cgggaacaaa accgccttat ccgtggactt ataaggcagc
     8341 gtgacgcatg gaagacacag gaaacagggc ataaagataa agcgtcagga cgcgcagaac
     8401 gcatcacagc gaagcgatta accgaccgtg accgcgaggt tatggaatgt ttccgcaatc
     8461 gctgatgagg ctgtatggcg gggtgggggg cgttttcagg acaaacccga ccccgccggg
     8521 caccgaacgc ctccccaaat ttttatgcac gggaattttt tgaaaaataa tctgacgaaa
     8581 aataagcatg gcaagaccac cgaaagcccc cgcctacctg gatgacatcg ccgtgaagca
     8641 gtggcgggaa aaatcgcggc agcttgcgga acggggagac ctgacccccg ccgactggag
     8701 caatctggaa ctgtattgcg tcaattactc gatataccgc aaagccgtcg cagaccttgc
     8761 ggcgcgcggg ttcagcattg ttaacagtca gggcggcgag agcagaaacc ccgcattaag
     8821 cgcgaaatcc gacgctgaaa gagtgatgat aaaaatggcc tccttgcttg gttttgaccc
     8881 gataagccgc cgtaaaaatc caccggaaac agaagaagag gacgagcttg accgcctgga
     8941 ataagtacgc agaagacgta aaaacgggca aaattccggc ctgtaaacgg ctgaaacagg
     9001 ccgttaaacg gtacttttcg gatcttaaaa gcccccttta cacgttcgat cgtgaggttg
     9061 tggagcggtt tattgccttt tccagggtgt gcccgcacgt aaaaggaccg atgcgtggca
     9121 gacccattga gctggagccg tggcagcagt tcgcctttgc gtgcatcctc ggctttaagg
     9181 ttaaggccac cggacggcgc aaatacacga gcgcctttat cgaagtgccg cgcaaaaatg
     9241 cgaaatcaac gaccgccgcg attctggcta actggtttct gattatggag aacggtcagc
     9301 aggatattta caccgcagcg gtgagccgtg accaggcgcg gatcgtcttt gatgatgcgc
     9361 gtcagatgtg ccttttatcc cgaccgttac gcaggcgggt gaatattcag gcgcataagg
     9421 tgatacaccc gaaaagcaac agcctgttaa agccgctggc agcaaaagcg gcaaccatcg
     9481 agggcacaaa ccccagcctt tccatcgtgg acgaatacca cctacacccc gataacggcg
     9541 tttattctgc gcttgagctg gggatgggcg cacgtcccga agggctgtta tttgccatta
     9601 ccacatcagg cagtaacgtc gtttcagcct gtaagcagca ctatgattat tgctgccaga
     9661 tcctggacgg cgaagaggtc aacgattcaa tttttgtact gatttacgaa ctggacgacg
     9721 aaaacgaggt tgatgatccg gcgatgtgga taaaagcgaa ccctaatatt gatgtttccg
     9781 tggatcgtga aaaactggcc tcaaccatcc agaaagcgcg gggtattccg tcgcagtggg
     9841 tggagatgat gacaaagcga tttaatatct ggtgtcaggg gactaccccg tggatgggta
     9901 acggcgcatg ggctgagtgt gccggaacgt tcacggagga agatttacac ggccaggagt
     9961 gttacgcggg gctggactta tcatcaacca gcgatatttc cagcgtgtgc tatgcctttc
    10021 cggtcggtaa aaccattatg ctggtttccc gtcactatct gccggaattt cagctacaga
    10081 accccgccaa taaaaaccgc gccatctatc gtcagtgggc taaagcaggc tggatacgca
    10141 caacgcctgg cgactgcatt gattacgacc ggatcagaga tgacatcatg caggacgcgg
    10201 agaaatttaa tatcaggctg gtgggctttg atacgtggaa cgccacgcat ctcagaacgc
    10261 aattacaggg ggcaggtttt gaggtggagc cgttcccgca aacctacctc agattcagtc
    10321 cggcggcgaa atcgttcgaa gtttttgtta accgcagggt gattgtgcat cgtggcgatc
    10381 cggtgttgtc ctggtcgatg agtaacgtcg tgatgcagag tgacgcgaac gccaatatca
    10441 agccgaacaa gaaaaaatca ccgaacaaga tagacccgag cgtggcggcg ctgatggcgt
    10501 ttggtacatt ccaggcagag catgaggatt ttgcttttga tataagcgac agccaccgcc
    10561 agaaactgga agaatttagc ggggtgtaat gaggtcagca gcatgacaga agccgaaata
    10621 acgggattaa tccgccgcgt tgccggaatc agccagcagg ctgacgaaca ggccacgcag
    10681 ccggacagcg tgacagccga aaactatgcg cgtgttgttg ctgaggtgat gcgccgtgat
    10741 ggtatagaac ttaatggcgt ggatatgcgc aacatacgaa ccagagctct tgagttgctg
    10801 gcctaccgtc gccgcgtgga gatgtatcgg gagaaggaga aaataacgta ccactggaag
    10861 aagccggagc ggttgcggcg gtaactggtt gatattcccg acggaccaaa aatgggtggc
    10921 tacatccctc gcttctgagg actgttaaca agctgcctga aagacagtca caaactacga
    10981 aattttcgca gtttaattaa tgatcagctt aatcgctaac ccgctgatat tttcggaaac
    11041 ctcaatttga ggaagtcggc gcggtaactc gctgaacttt aagcaaagtg caaaattgcg
    11101 cttgctgaat aatcattatg attacgcaga tgattaagga atgaccgaag gcggaaattc
    11161 gcctgtggtt aatgggggag ttgcagatct gcaactcgac catgaaacta cggaaactac
    11221 ccgtagtttg ggtagtaaga gtaacaccca gattttgggt cttactcgtg atacccaaat
    11281 ttggggtatc agtggcaacc ataacgactt tcgttacggt tgatgctttt accccattgg
    11341 gggaaaggta ttacgataat cataacacct accgaacagg taaagcccac cagcctgatt
    11401 aacgtttaac cggaaaaaaa ttccggtagg tggggatccc catatcgaca ttaacgcccc
    11461 tcatgaattg gtgcgcttcc ccctggaaag atgatccgcc tgtatatttc ttgtgtctat
    11521 ttgttccacg ttgtttcata cagtgcaccg aacggtgtag tcactggtgt agtcactttg
    11581 cgattttt
//
LOCUS       DAZGOB010000144        11415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGOB010000144 DAZGOB010000000
VERSION     DAZGOB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.144"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            130..747
                     /gene="yijF"
                     /locus_tag="ACHLIF_004237"
     CDS             130..747
                     /gene="yijF"
                     /locus_tag="ACHLIF_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO9608135.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(774..1679)
                     /gene="yijE"
                     /locus_tag="ACHLIF_004238"
     CDS             complement(774..1679)
                     /gene="yijE"
                     /locus_tag="ACHLIF_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO9608136.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(1773..3953)
                     /gene="katG"
                     /locus_tag="ACHLIF_004239"
     CDS             complement(1773..3953)
                     /gene="katG"
                     /locus_tag="ACHLIF_004239"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO9608137.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4282..5172)
                     /gene="metF"
                     /locus_tag="ACHLIF_004240"
     CDS             complement(4282..5172)
                     /gene="metF"
                     /locus_tag="ACHLIF_004240"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO9608138.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5248..5382
                     /locus_tag="ACHLIF_004241"
     CDS             5248..5382
                     /locus_tag="ACHLIF_004241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608139.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(5521..7953)
                     /gene="metL"
                     /locus_tag="ACHLIF_004242"
     CDS             complement(5521..7953)
                     /gene="metL"
                     /locus_tag="ACHLIF_004242"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO9608140.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPVSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(7956..9116)
                     /gene="metB"
                     /locus_tag="ACHLIF_004243"
     CDS             complement(7956..9116)
                     /gene="metB"
                     /locus_tag="ACHLIF_004243"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO9608141.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            9393..9710
                     /gene="metJ"
                     /locus_tag="ACHLIF_004244"
     CDS             9393..9710
                     /gene="metJ"
                     /locus_tag="ACHLIF_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO9608142.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9894..10502
                     /gene="yiiX"
                     /locus_tag="ACHLIF_004245"
     CDS             9894..10502
                     /gene="yiiX"
                     /locus_tag="ACHLIF_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO9608143.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            10647..11322
                     /locus_tag="ACHLIF_004246"
                     /pseudo
     CDS             10647..11322
                     /locus_tag="ACHLIF_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2797 a         3185 c         2877 g         2556 t
ORIGIN      
        1 tctgtgccag taatctgaaa ctcctacgtt gttcaggagt tttttatccg cttctggaca
       61 ttttctctac agaacaatcg ttagcccgga aggtcgaatc tgtgactctt attttcacgg
      121 acgaaccaga tgaaagcttc actggcgctg ctcagcctgc taacggcatt taccagccac
      181 tccttaaaat ctcccgccgt tccaccaacc gtggtacaaa tccaggccaa taccaacctt
      241 gccatcgcag acggtgccag acagcaaatt ggcagcacgc tattttacga tccggcgtat
      301 atgcagctta cctatcctgg cggtgatgtc ccgcaagaac gcggggtatg ttccgatgtg
      361 gtgatccgtg cattgcgcag ccagaaggtc gatttgcaga aactggtgca cgaagatatg
      421 gcgaagaatt ttgccgagta cccgcaaaaa tggcagttaa agcgcccgga cagcaacatc
      481 gatcaccgtc gggtgcctaa tctggaaacc tggtttaccc gccacgataa aacacgcccc
      541 atcagcaaga accccagtga ctatcaagcg ggcgatattg tctcctggcg actggataac
      601 ggactggcac atattggggt ggtgtcagat ggcttcgccc gcgacggtac gccgctggtg
      661 atacataaca ttggcgcagg cgcgcaggaa gaagatgtgc tgtttagctg gcggatggtg
      721 gggcattatc gttattttgt taaataagcg atcgccccac atgaagaaaa aattcagatg
      781 ctttttacac tgacagcttc ttttttctta cggctcacca gcgccagtgc cagcacaatc
      841 agcacaatac cgctaccttc aacggccccc ggattctcac cgagcagcca ccaggaaaag
      901 agtacgccgc aaacgggaac ggccagcgtg cttaagctgg caatactggc aggcaagttt
      961 ttcaatacaa acaaccataa gctccacgcc agcgccgtcg ccagaatcgc actgtaggcc
     1021 agcgcccaga acacggtggg ctgccagtca atttcacgtt gcggcaccag taaagcgacc
     1081 acactcatca ccagcgccgc atagagcatc tgccaggatg ttaacgacaa taaatccacg
     1141 cgcggatgac gggcatacag acgtttagca acaatcgcgc tcgcccccca actgacgccg
     1201 gagaggattg ccagcatggc acttttcatc gaagagaaat cgagttgcca cggctgcaac
     1261 accaaaaata agccgaaagc ggcaatcagt atcgcgaaat attgcccacg tcgcaggcgt
     1321 tcaccgagaa acaacgcggc gaaaatcacc acccagaacg gcatggtata gcttaggatc
     1381 gccaccttcc ccgctccgcc gctgaccagc gcccactgcg ccaggccaac catcccgcag
     1441 gtttgtaaca gggcaatggc taaggtgtat ttaaacggtg tcgggcgcat tccgcgacca
     1501 cgtaataaaa ggacgatgaa taaaacgaga gcgccgaaaa tgcagcgtaa ggcggtaaag
     1561 tcgaaggcac cgatgtagct ggtgacttgc ttcatgaaaa tccagctata actccagata
     1621 agtgtgagca caaccaggcc actgattgcc agtgggttgc tctttcctgc gacagacatg
     1681 agagatccag tgtgtagttt tcctgtggac actatacgcc ggtaaagaaa cttcagcgac
     1741 tgcctgaaag gcagtcgctg aagaaggtca gattacagca ggtcgaaacg gtcgaggttc
     1801 atcactttca cccatgccgc cacgaagtct ttaacaaact tctcgtgagc atcgctgctg
     1861 gcgtaaactt cagccaccgc acgcaggaca gagttagaac caaataccag atccgcacgg
     1921 ctggcagtgt atttcacttc gccggtttcg cggtcacggc cttcaaacag ctctttcgat
     1981 tcgtcggtcg ctttccactc gtaacgcata tccagcaagt tcacgaagaa gtcattgctc
     2041 aatacgccaa cgcggtcagt gaagacgccg tttttgctgc catcgaagtt ggcacccagt
     2101 acacgcatcc cgcccaccag cgcggtcatt tccggcgcgg tcagggtcag ttgctgggct
     2161 ttgtcgatca gcagtgactc ggtggtggaa acatccagac gagcgcgata gttacggaaa
     2221 ccgtcagcaa ttggctccag cagctcgaac atctcaatgt cagtctgatc ctgacgcgca
     2281 tcaacgcggc ccggcgcaaa cggtacatga atgctcaaac ctgcggcgct tgcggctttc
     2341 tcaacaccaa ccacaccagc cagcacgatg atatccgcca gcgaggcttt accagactct
     2401 ttctggattt tctccaaaac aggcagagca cgaacggctg cggcgttcac atcccagtcg
     2461 cgctgcggca ttaatgccag acgcgcaccg ttggcaccac cgcgtttgtc gccaccacgg
     2521 aaggtagaag cagaagccca ggccaccgat accagctcac taacagacag accagaatcc
     2581 gcaatcgcga atttcagatc gataatgtcc tgctcggtcg ggttgtagat cggctgcggc
     2641 agcggatctt gccagatcag atcttctttc ggcacttccg gcccgatgta gcgagatttc
     2701 ggccccatat ccctgtgcgt cagtttgaac caggcacggg caaaggcttc gttgaacgcc
     2761 tgcggatcgt tgaggaaacg acgagagatc ttctcgaact caggatcaaa acgcagcgtc
     2821 aggtcggtca ccagcatcgt cggtttacgt ttcttcgacg gatcaaacgg atccgggata
     2881 atttccggtg cgtctaccgc ttcgaactgg attgcgccag ccgggctgcg ggtctgtacc
     2941 cactcatact tgaacaggtt ctcgaagaaa tagttgctcc actgggtcgg cgtctgggtc
     3001 cagactactt ccagaccaga ggtaatggca tctgcgccaa cgccgctgcc gtaagtgctc
     3061 gcccaaccta aaccttgttc ttcaatcggt gcagcttctg gatcaggacc tacatttgat
     3121 gtcggaccgg caccgtgggt tttacccagc gtatgaccac ccgcaataag cgccacggtt
     3181 tcttcgtcgt tcatgcccat gttgccgaag gtcgcacgga tagctgctgc cgcagaaagc
     3241 ggttcgccgc tgtgatccgg gccttccggg ttaacgtaaa tcaggcccat ctcggttgca
     3301 cccagcggtg ctttcgccag cgcttccgga tgacggtggg tcagccaggc tttttcatca
     3361 ccccagttaa catccagatc cggttcccag acgtcttcac gaccggcacc aaaaccgaag
     3421 gtacggaagc cggagttttc cagcgccacg ttacccgcga ggataaacag gtcggcccag
     3481 gagattttct gaccatattt ctgtttgatt ggccacaaca ggcgacgcgc tttatcgagg
     3541 cttacgttat ccggccagga gttcagcggt gcaaaacgtt gctgaccacg acccgcgccg
     3601 ccgcgtccat cgattgaacg gtaagtcccc gcgccgtgcc aggccatacg aataaacaga
     3661 ccggcgtagc tgccccagtc ggcaggccac cacggctgag attctgtcag cagggctttc
     3721 agatcttttt tcaggccgta gtaatctaat ttgctgaatt ctttgcggta gtcaaagtcc
     3781 tcacccagcg ggttagaacg attagaatgt tggtttaaca ggtcaacacg gagttgattt
     3841 ggccaccagt cgcgagtggt tgtgcccgcc cccgcgctct ggtcgtgacc gccctgatgg
     3901 aacgggcatt tgccagtggc tgtcgtgtta tgaatatcgt ctgacgtgct catcaatgtg
     3961 ctcccctcta cagtgttacc gttacgatac acagcgttag agagaagtta taattgaatt
     4021 aatccacgga tgcgatagtt gagatcttac atattgttgg ttaaagagat gtagatcaaa
     4081 ttgatcgtaa ttaagctttt ttgattatgt ccgaattcgg acaactgctt tataaaaaag
     4141 ggttttagat tcctattctt caaggaagga gggcgaaaac aaagctgaca accttatttt
     4201 tacgccctca tttcgaggca gcattttgtg ctctgtttaa aatttgtgat cacagtgtga
     4261 ttttcacaaa agccacacta tttataaacc aggtcgaaca cccagcgtat ggcaaatcgc
     4321 gtaactcatt tcagcgcggt taagcgtata gaagtggaaa tctttcactc cttcacggct
     4381 taaaatcttc accatatcca tggcaatatt cgcgcccacc agcttgcgcg tttcagcatc
     4441 gtcatccagc ccgtcaaaca tttgtgccat ccacgccgga atacgcacgt tggtcatatc
     4501 ggcaaatttc ttcgcctgtt taaagttaga taccggcaaa atccccggaa taatttccac
     4561 atcaatgccc gccgatacgc agcggtcacg aaaacgcagg tagctttcga catcaaagaa
     4621 gaactgagta atcgcgcggt tggctccggc atcgactttg cgtttcaggt tgagcaaatc
     4681 cgcctgagcg ctttttgctt ccgggtgaac ttccggatac gccgccacgg agatatcgaa
     4741 atctgccact tcttttaaca gcgtcaccag gtcagaagca tacatttccg gcttaccact
     4801 tcccggcggc agatcgccac gcagcgccac gatatgacga ataccgttat tccagtagtc
     4861 gcgtgcaatg gtgcgcagct cgtcgggcgt cgcatcaatg caagtaagat gcggtgccgc
     4921 ttccagacca gtgcgatctt taatgccttt aataatgctg tgcgtacggt cgcgctcgcc
     4981 ggagttcgcg ccataggtca ccgatacaaa cttcggtttc aggctgctaa ggcgatcgat
     5041 ggagttccac agggtctgct ccatttcact ggtacgcggc gggaaaaact cgaacgaaac
     5101 gttaatctgc ccctggactt ctgccaggct ctgattcagg gcatcccgct ggctggcgtg
     5161 aaaaaagctc ataccttacc tcatcaatcg cttgtagtta tatgttgtgt tgtgcacatc
     5221 tatccattta gacgtccaga tgtaaagatg aaggaaaagc cgaagggcgt caaccgaaaa
     5281 atgaccaatg atggtgagga aaaatcaggg aagatgaaaa aacttcagga tggtaagaaa
     5341 aagaaaatcc ggtatgcaaa gcaaaccgga ctgcaaatat aaactaacca tgtacttatt
     5401 gcttcttgcc ggattcgtcg taaacgcttc atccggccta atcctatgat aattcaatat
     5461 atttcattag aaaccataga cccgaaaaca tgagtaccgg gcattattaa atttctgaaa
     5521 ttacaacaac tgtgccagcc ggttgatatc cgactgaatc gccccggcgg tgacgtcgcg
     5581 cccagcgcca ggcccacgga tcaccagcgg attatcgcgg taccagcggc tttcgatggc
     5641 aaagacgtta tcgcatggca gcagtgatgc cagcggatgg tcttcacgca ccgcttccac
     5701 gcccacacgc gctttgccat tggcatcgaa acgcgccacg tagcgcagca ccagccccat
     5761 ttcgcgggcc gcttccagcc gttgcaccat ctgctcgtta agttcatcgc cattttcaaa
     5821 gaaatggtcg atgctgccgc cttcgcaatg agcaggcacc agcgattcca cacgtacctg
     5881 gtccggttca atgttgtaac ccgcttcacg cgccagaatt accagcttgc gcattacgtc
     5941 tttgccggag aggtcgtcac gcggatcagg ctcggttaag ccctgctgcc acgcctgatc
     6001 caccagctcg gtaaacggca cgctaccgtc gaattgcagg aacagccagg agagcgtgcc
     6061 ggagaagatc ccgctgatcg acaaaatagt atcgccgctg tcgatcagat cgcgcacggt
     6121 gtggttgatc ggcaagcccg caccgacggt ggcattgtac agccagtgac gcccggtttt
     6181 ttcgaaggcg tcgtggatct ggcgatattt attgctgtcg ctggctcccg ccagtttgtt
     6241 ggcgctgata acgtggaaac cgtggctggc gaaatcaaga tactgatcag caagctgctg
     6301 gctggcggta acgtccagca ccactaaatc atcatacgga tgggcgcgca tccacaggaa
     6361 caacgactct tcatcctgct caaccgcttc atcgttgaag aaggctaacg cgcggctggc
     6421 gtccagcccg tcatagctca acaggctgcg gcggctgtcc accacacctg ccagcacaaa
     6481 ctcaaagccg gtacgtgccg aaagcgtgct ctgctcacgg gcgaacagtt ccagccaacg
     6541 ggaaccgata ttgcccttac cgaacaatac caggccgatg cgtttttctg cgcggaagac
     6601 ggactgatgc agcccctgaa tcaggctttc ggtcgggccg gtgcgcagta ctgccaccag
     6661 gctgatgccg tcatcggact gccaggtaaa ttcgaccggc tggcctttca gttgctgcca
     6721 gaagcggtgg caatgcagcg ggttacgggt gacgcctgca ccgaccatcg ccaccagcgc
     6781 cagcccctga cgcaggcgca gttcgccagg taatcccgct tcgtcgagga ttttcagcgc
     6841 actgtcggcc acttctgagg tgtagcaaaa ttgcagcaac tggcgatcgt tatgtacgcc
     6901 aaccgccagc gggcgtacct gcgcgcgttt caggatttgg tcgatctctt tatgcgccag
     6961 tttgaaatcc tgactgacgg gcacctgaaa ctcaatcaaa cagacatcat cgtggctggt
     7021 gacaatacgc gcaccagtac cggaggccag cacgcgttca atgcgcgtgg aaccttgatc
     7081 cggcgtgtag ctacagcgca gttgcaggtc gatttcgctg ccagaaaccg gctgtaaagt
     7141 acgggcgtga agaacgggag ccgccaggcg cgccagttcg ctggcctcat ccagacgcag
     7201 caacggcagc aggcaggcat ctttcacttt acgcgggtcg gcactgtata ccccggcgac
     7261 gtcgctccag atggttacgc gagaaacacc cgccagcgca ccgatttgtg tcgcggaata
     7321 gtcggaaccg ttacgcccca gcagcaccgt ttcaccggcg ttgttgcggc tgataaatcc
     7381 ggtcaccacc agacgtttgc ccggatgttg caccagcagc tgttgcagca acgggtaaga
     7441 aagcccttca tcaacctgcg gttgtgcggc gcgttcagcg cgtaaaaact cgcgggcatc
     7501 aagccaggcc gctggcagcc cttgttgatt aagtaccgca gacatcaggc gtgccgacca
     7561 tacttccccg tggcccacca cttccgcata cactgcgtcg ttaataccgc tgtcgagcag
     7621 cgccgccagg cgctcaaggt cgctgacaaa agcgctaatg aggctatcgg cttcttcagc
     7681 gggtagcaga ccgctaatca gatcgcactg atagcgacgc agcgtttgtt gaacctgatg
     7741 cgcagagaga cgatcggtct ggcttagttt caaccagtta atcaactggt tagtggtgct
     7801 accggcggcg gaaaccacca tcatatcgtc aggctgagag tactccgcca taatgcccgc
     7861 gacacgcaaa taacacttca catcagccag actactgcca ccaaatttat gcagctgacg
     7921 acctttcgcc cctgcctgcg caatcacact catttttacc ccttgtttgc agcccggaag
     7981 ccattttcca agtcggcaat taaatcttcg ccatcttcaa taccggtgga gatacgcagc
     8041 agcgtctcgg aaatcccggc ggcagcacgc gcttctggtg ccatgcctgc atgtgtcatg
     8101 gttgcggcgt gagagattaa actttccact ccccctaatg attccgccag cgtaaacaac
     8161 gacagcccgc ccaggaaacg acgcagcgtc tgctcatcgc catccagttc aaaactcaac
     8221 attgcgccaa agcctttttg ctgacgcgcg gcaatttcat gcccctgatt ttccggcagc
     8281 gacgggtgat acagtttttt caccaacggc tgggtttgca gatatttcac aatcgcctgc
     8341 gcgttgcgct gcgccagctc catacgcggc accagcgttc gcaacccgcg tagcagcaga
     8401 tagctgtcaa acgcgccgcc cgtcacgcca atattgtttg cccaccaggc aagttcagtg
     8461 acaacgtccg ggtctttagc aatcaccacg ccagccacca cgtcagagtg accgttcaga
     8521 tatttcgtac atgaatgcaa caccagatcg gcacccaatg ccagcggatt ttgtaatgcc
     8581 gggcttaaga aggtgttatc caccacgctc accgccccga cttcccttgc cagatggcag
     8641 attttcgcaa tatccacgac gcgtaacaat ggattacttg ggctttctac cagtaccagt
     8701 ttgggttttt ctgccagtgc tgcccgtaat gcctgttcat cgccttgatc aacaaacaac
     8761 acgcgatagc aaccgcgttt cgccaggctg tcgaacaggc gatagctacc gccgtagcag
     8821 tcgtgcggcg caaccagcag atcgccaggt ttcaaaaaga cggtcattac caggtgaatc
     8881 gcggacatgc cggtattagt aagtactgca ccagcaccgc cttccagttc tgccagcgca
     8941 cgctgaacca catcgcgcgt tgggttgccg cgacgcgagt aatcatgcgc gcgcggttca
     9001 ttaaatccgg taaagttata tgtgctggaa agatggatcg gtgggacaac gcaaccatac
     9061 tgttcgtcgt catttaaccc gctacgcact gcgatggtgg cctgtttacg cgtcatgtga
     9121 tgaagttccc tgggctttgt cggtgaaatg tcaggcacca gagtaaacat tgtgttaatg
     9181 gacgtcaata catctggaca tctaaacttc tttgcgtata gattgagcaa atcccaaata
     9241 gccgttaaaa ttatatgcat tatcacgccg acaggtgcat tacacgatgt cacggtaacg
     9301 cctgtacggt aaactatgcg ggtttacggt cagtacccac ataaattgtg tggtctggtc
     9361 tcaatttatt gacgaagagg attaagtatc tcatggctga atggagcggc gaatatatca
     9421 gcccatacgc tgagcacggc aagaagagtg aacaagtcaa aaagattacg gtttccattc
     9481 ctcttaaggt gttaaaaatc ctcaccgatg aacgcacgcg tcgtcaggtg aacaacctgc
     9541 gtcacgctac caacagcgag ctgctgtgcg aagcgtttct gcatgccttt accgggcaac
     9601 ctttgccgga tgatgccgat ctgcgtaaag agcgcagcga cgaaatcccg gaagcggcaa
     9661 aagagatcat gcgtgagatg gggattaacc cggagacgtg ggaatactaa agcggaaatg
     9721 agcggcgcgg agtggaatcg cctgatgcgc tacgcttatc aggcctacgt catattgcaa
     9781 tttattgaat ttgcacgaac ttgtaggccg gataaggcgt tcacgccgca tccggcataa
     9841 acaacgagca cgttgtctgc gacccaccgc tttttataca tggacgttta actatgaaaa
     9901 acaggctgct gatcctcagc ctgctggttt ctgtacctgc ctttgcctgg cagccgcaaa
     9961 ctggcgacat catctttcaa atctctcgct catcgcaaag taaagcgatc caactggcaa
    10021 cccattccga ttacagccac accggtatgc tggtgatgcg taacaaaaag ccctacattt
    10081 ttgaagcggt cggcccggtg aaatacaccc cgctcaagca gtggattgcc catggtgaaa
    10141 agggcaaata cgtcgttcgc cgcgttgaag gcggactgag tgttgaacaa cagcaaaaac
    10201 tggcgcaaac ggcaaaacgt tatctcggta aaccgtatga tttcagcttc tcatggagcg
    10261 acgatcgcca gtactgttcg gaagtggtgt ggaaggttta ccagaacgcg ctgggaatgc
    10321 gcgtgggcga acagcagaag ttgaaagagt ttgatctcag caacccgctg gtccaggcga
    10381 agctcaaaga acgctacggt aaaaatattc cgcttgagga aacggtagtt tcaccacaag
    10441 ccgttttcga cgcgccacaa ctcaccacgg ttgccaaaga gtggccgctg ttttcgtggt
    10501 aatggatgat gaaaaaaata attcccgccg tagtctgttt gttgctttgt agttgcacga
    10561 cctttccgca agctgtcgct ccggtaaacg gtgatgcctc caattagttg aattaaggat
    10621 ataatactcg cttttaaggt tgtttcatgg tcacagttaa ccttcattgc ccccgttgcc
    10681 agtccgttca ggtttaccgt cacgggcaga acccaaaaag acatgatcga ttccgcggcc
    10741 gtgactgtca ccgggtattt cagctcagtt actgttacga gccccgtaaa ccgggagtta
    10801 aagaccaaat cgctgaaatg actttcaacg gtgccggggt tcgcgatact gcccgtacgc
    10861 tgaaaatcgg cattaataca gtcatccgaa ctttaaaaaa ctctcgccac gaagaataac
    10921 gtcatccccg gtcgctcatg ctgatgtggc actcatctgc aaacttagtc agcaatggag
    10981 ctttgtcggc agtaaagcgc gacagcactg gctctggtac gtgtacaaca cgaaaactgg
    11041 tggtgttctg gcctacactt ttggcccccg gactgacgaa acctgtcgtg aattgcgggc
    11101 gctgcttacc cttttaccat cggcatgttg accactgacg aatggggtag ttacgctaga
    11161 gagttaccga aggagaagca tctgactggt aagattttta ctcagcacat tgagcgtaac
    11221 aatctgaccc tgagaacccg aatcaaacgt ctggcccgca gaaccatctg tttctcgcgt
    11281 tctgtagaac ttcatgaaaa agtcatcggt gcttttatcg aaaatatatg ttctactaat
    11341 tggagtcatc acccttctga taagctttcc ttggatgatt accagactga atacacagcc
    11401 aaagattttt acctc
//
LOCUS       DAZGOB010000145        11414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGOB010000145 DAZGOB010000000
VERSION     DAZGOB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.145"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            295..1683
                     /gene="narU"
                     /locus_tag="ACHLIF_004247"
     CDS             295..1683
                     /gene="narU"
                     /locus_tag="ACHLIF_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO9608144.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1765..5505
                     /gene="narZ"
                     /locus_tag="ACHLIF_004248"
     CDS             1765..5505
                     /gene="narZ"
                     /locus_tag="ACHLIF_004248"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO9608145.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5502..7046
                     /gene="narH"
                     /locus_tag="ACHLIF_004249"
     CDS             5502..7046
                     /gene="narH"
                     /locus_tag="ACHLIF_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO9608146.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7046..7741
                     /gene="narW"
                     /locus_tag="ACHLIF_004250"
     CDS             7046..7741
                     /gene="narW"
                     /locus_tag="ACHLIF_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO9608147.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7738..8418
                     /gene="narI"
                     /locus_tag="ACHLIF_004251"
     CDS             7738..8418
                     /gene="narI"
                     /locus_tag="ACHLIF_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO9608148.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKREMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8497..9390
                     /locus_tag="ACHLIF_004252"
     CDS             8497..9390
                     /locus_tag="ACHLIF_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO9608149.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9486..10331)
                     /gene="nhoA"
                     /locus_tag="ACHLIF_004253"
     CDS             complement(9486..10331)
                     /gene="nhoA"
                     /locus_tag="ACHLIF_004253"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO9608150.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10504..11073
                     /locus_tag="ACHLIF_004254"
     CDS             10504..11073
                     /locus_tag="ACHLIF_004254"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO9608151.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11077..11304)
                     /gene="pptA"
                     /locus_tag="ACHLIF_004255"
                     /pseudo
     CDS             complement(11077..11304)
                     /gene="pptA"
                     /locus_tag="ACHLIF_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
BASE COUNT         2689 a         2964 c         3018 g         2743 t
ORIGIN      
        1 tctggtcttg ttgataatat agttaatatg taacccttta gattcacgat ctgaagggtt
       61 gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt tagttacatt
      121 tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg ctttcttatc
      181 tattctttca atcagcactt cacccaattc atccttaatt ctaataactg gctattgcgc
      241 cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa caatatggca
      301 ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga aaatccggcc
      361 ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc agtcagttgt
      421 ctacttcttg ccttctgtgt ctggatgcta tttagcgcag ttaccgttaa tctcaataaa
      481 atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc ctccgtttct
      541 ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg acgccgatgg
      601 acggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat tgccgtgcaa
      661 aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg ttttgcaggt
      721 gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc caaacaaggg
      781 agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat gcagctggtt
      841 gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg cgtaccgcag
      901 gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc gctactggcg
      961 attgccacga tcgccgcatg gtcagggatg aatgatatcg ccagttcacg cgcctcaatt
     1021 gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct gctttacctt
     1081 gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc aaaaacccag
     1141 ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttatcgg tgccatcgcg
     1201 cggtcggttg gtggtgctat ttccgataag ttcggcggcg tgcgggtgac gttgatcaac
     1261 tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg cacaggctcc
     1321 ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgactgc gggtctggga
     1381 agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat ttatcgggta
     1441 aagatgaaag gcggtagtga tgagcaagct cagaaagaag ccgtcaccga aacggcggcg
     1501 gctctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc gcaggcgttt
     1561 ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt tttaatcttc
     1621 tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt cagccaaaaa
     1681 taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca gatcccaatt
     1741 cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa
     1801 caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg
     1861 gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt
     1921 aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt gacctgggaa
     1981 atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga acctcgcggc
     2041 tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct gaaatacccg
     2101 ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaagcaaca cagcgatccg
     2161 gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta caaacaagtg
     2221 cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct gattgccgcc
     2281 gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt ctcgccgatc
     2341 ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct gcttggcggc
     2401 acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg
     2461 ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta catcatcgcc
     2521 tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac cgaagtacgc
     2581 tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc caaattgtgc
     2641 gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc aatgggccat
     2701 gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa ctactgccgc
     2761 cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg tagctacgtt
     2821 cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag caacaatccg
     2881 cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa cggttcgatt
     2941 ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc cggtacggaa
     3001 accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt ggccttcccc
     3061 tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc ggtgctggtg
     3121 cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc ggtggtcagc
     3181 gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga tgaaaacagt
     3241 gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca aattaccggc
     3301 gtgccgcgcc agtatattga aaccatcgcc cgtgaatttg ccgataccgc ccataaaacg
     3361 catgggcgct cgatgattat cctcggcgca ggtgttaacc actggtatca catggacatg
     3421 aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca aagcggtggc
     3481 ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg gttgccgctg
     3541 gcctttgcgc tcgactggaa ccgaccacca cgccagatga acagtacctc gtttttctac
     3601 aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact ttcaccgctc
     3661 gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc cgaacgtatg
     3721 ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa agctgaagcc
     3781 gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc gggcgattta
     3841 cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt gtttgtctgg
     3901 cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa gtatctgctg
     3961 gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa
     4021 gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt gacgctcgac
     4081 ttccgcatgt ccagtacctg cctgttctcc gatatcgttc tgcccaccgc cacctggtac
     4141 gaaaaagacg atatgaacac ctcggatatg catccgttta ttcatccgct ttctgcggcg
     4201 gtcgatccgg cgtgggaatc acgcagcgac tgggaaatct acaaaggtat cgccaaagca
     4261 ttttcgcaag tgtgcgtggg tcatcttggc aaagaaaccg acgtggtatt acaaccccta
     4321 ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc
     4381 gaatgcgatc tgattccagg caaaaccgcg ccgaatattg tggcggtgga gcgcgactac
     4441 cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact tggcaatggc
     4501 ggtaaaggga tttcgtggaa tacgcaggat gaaatcgatt tcctcggcaa actcaattac
     4561 accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat tgacgcttca
     4621 gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa agcgtggcag
     4681 gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa agaggacgag
     4741 aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag ccccacctgg
     4801 tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt tcatgagtta
     4861 attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc gtggatgcgt
     4921 gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag cgtcagtgag
     4981 atgcgccaga taccgccaaa cggcttcccg gaaaaagcac ttaacttcct gacgccgcac
     5041 cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac gctctctcgc
     5101 ggtggaccga ttgtctggat cagcgaaaca gatgcccgag aactgaccat tgtcgataac
     5161 gactgggtgg aagtattcaa cgccaacggc gcgctgactg cccgcgcggt ggtcagccaa
     5221 cgtgtaccgc cgggcatgac catgatgtat cacgcccagg aacgcattat gaatattcct
     5281 ggttcggaag taactggcat gcgcggcggc attcataact cggtcacccg cgtttgcccg
     5341 aaaccaacgc atatgattgg cggttacgcg cagctggcct ggggctttaa ctactacggc
     5401 accgtcggat cgaaccgcga tgagttcatc atgatccgca agatgaagaa cgttaactgg
     5461 ctggatgatg aaggtcgcga tcaggtacag gaggcgaaaa aatgaaaatc cgttcacaag
     5521 tcggcatggt gcttaacctc gacaaatgta tcggctgcca tacctgttcg gtgacctgta
     5581 aaaacgtctg gaccgggcgc gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca
     5641 aaccgggcat tggttatccg aaaaactggg aagatcagga agagtggcaa ggcggctggg
     5701 tccgcgatgt gaatggcaag atccgtccgc gtctgggcag caagatgggc gtaataacca
     5761 aaatcttcgc caacccggtg gtgccgcaga ttgatgatta ctacgaacct ttcaccttcg
     5821 actacgaaca tttgcatagc gcaccggaag gcaaacatat ccctactgct cgcccgcgtt
     5881 cactgattga cggcaaacgg atggacaaag tgatctgggg cccaaactgg gaagaactgc
     5941 tgggcggcga gtttgaaaaa cgtgcccgcg accgcaactt cgaggccatg caaaaggaga
     6001 tgtacgggca gtttgaaaac accttcatga tgtacctgcc gcgcctgtgc gaacactgcc
     6061 tcaatcccag ttgcgtggcg acctgcccaa gcggcgctat ctacaaacgc gaagaagacg
     6121 gcattgtgct gattgatcag gataaatgcc gtggctggcg tttgtgcatt agcggttgtc
     6181 cgtacaaaaa aatctacttc aactggaaaa gcggcaagtc agaaaaatgt atcttctgtt
     6241 atccgcgaat tgagtcaggc caacccaccg tgtgctcaga aacctgcgtg ggtcgcatcc
     6301 ggtatctggg cgtgctgctt tacgacgccg accgcattga ggaagcggcg agcaccgagc
     6361 gcgaagttga cctctatgaa cgtcagtgcg aagtattcct cgatccacac gatccctcag
     6421 tgatcgagga agccctgaaa caaggtattc cacaaaacgt gattgaagct gcccagcgtt
     6481 cgcctgtcta caaaatggcg atggactgga aactggcgct accgctgcac cctgaatatc
     6541 gcaccctgcc aatggtctgg tacgttcctc cgctgtcgcc gattcagtcc tacgcagatg
     6601 cgggcggttt gccgaaaagc gaaggcgtgc tgcccgccat cgaaagcctg cgtattccgg
     6661 tgcaatatct cgccaatatg ttgagtgccg gcgataccgg tccggtactg cgggcgctga
     6721 aacggatgat ggcgatgcgc cactatatgc gttcacaaac cgtggaaggc gttactgata
     6781 ctcgtgccat cgacgaagta ggcctgagcg tcgcccaggt cgaagagatg tatcgttacc
     6841 tcgccattgc caactatgaa gatcgttttg tcatcccgac gagccatcgg gaaatggcgg
     6901 gcgatgcctt cgcagaacgc aacggctgcg gttttacctt tggcgacggt tgccacggtt
     6961 cggacagtaa attcaacctg ttcaacagta gccgtatcga tgccatcaac atcaccgaag
     7021 tgcgcgacaa agcggagggc gaataatgca gatcctcaaa gtgatcggcc tgttgatgga
     7081 gtatccggac gagctgttgt gggaatgcaa ggaggacgcg ctggcgttga tccgcagcga
     7141 cgcgccgatg cttacggatt tcacccacaa cctgcttaac gcgccgctgc tggataaaca
     7201 ggccgaatgg tgcgaagtgt ttgaccgtgg acgtaccaca tcgctgctgc tgttcgaaca
     7261 tgttcatgcc gagtcccgcg atcgcggcca ggcaatggtg gacctgctgg cggagtatga
     7321 aaaggtcggc ctgcagctgg attgtcggga actgcccgat tatctaccgc tgtatctgga
     7381 gtatttaagc gtactgcctg acgatcaggc gaaagaagga ttgctcaatg ttgcgccgat
     7441 cctcgccctg cttggcggtc gcttaaaaca acgcgaggca ccgtggtacg cgttgtttga
     7501 tgctctgctg caactggcgg gaagctccct ttcaagtgac agtgtcacaa aacaagtaaa
     7561 cagcgaagag cgcgatgaca cccgccaggc gcttgatgca gtgtgggaag aggaacaggt
     7621 gaagtttatt gaagataacg ccacggcgtg tgacagctcg ccgcttaatc aatatcagcg
     7681 acgctttagc caggatgtcg cgccgcaata tgtcgacatc agtgcgggag gtgggaaatg
     7741 attcagtatc tgaacgtctt tttttacgat atctacccgt acatttgtgc gacggtgttt
     7801 ttcctcggca gctggctgcg ctacgactac gggcagtaca cctggcgcgc ctcctcaagt
     7861 caaatgctcg ataaacgcga gatggtgata tggtcgaatc tgttccatat cggcattttg
     7921 gggattttct tcgggcactt gttcggcatg ttaacgccgc actggatgta cgcgtggttt
     7981 ttgccagtgg cagcgaaaca actaatggcg atggtgctcg gcggtatttg cggtgtgttg
     8041 acattgattg gcggcgcagg actgctgtgg cgcaggctga caaatcagcg cgtgcgtgca
     8101 acttccacta cgccggatat tatcatcatg agcattttgc tgatccagtg tctgctgggg
     8161 ctaagtacca taccgttttc agcacagtat cctgatggta gcgaaatgat gaagctggtg
     8221 ggttgggcgc aaagcattgt cactttccgt ggtggatcgt cagaaatgct taacggcgta
     8281 gcgttcgttt tccgcctgca tctggtattg ggaatgacca tcttcctgct cttcccgttc
     8341 accagattgg tgcatgtgtg gagcgcgccg tttgagtact ttactcgtcg gtatcagatt
     8401 gtgcgttcgc ggcgataatt tcaccctggc cggatagccc ttcgctctcc ggccacttgc
     8461 aggctatgat agttctccag tcacaggaga acgttaatga aaccgcaggt gtaccacgtc
     8521 gatgctttta cctctcaacc atttcgcggc aattctgccg gtgttgtttt ccccgccgat
     8581 aatcttagcg aagcgcaaat gcagcttatc gcccgcgagt taggccattc ggaaactgct
     8641 tttctgctgc acagcgacga cagcgatgtg cgcatccgct actttacgcc aacggttgaa
     8701 gtgccgattt gcggtcacgc gacagtagct gcgcactatg tacgtgcgaa ggtgttaggt
     8761 ttaggaaatt gcacgatctg gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa
     8821 aagcacaacg atgattatcg tatttcgctg gaacaaggta cgccgggctt tgagccaccg
     8881 ctggaaggtg aaacacgtga ggcgattatc aacgcgcttc atctcactga agacgatatt
     8941 ttgccgggct tgccaattca ggtcgcaaca acaggacact caaaagtgat gatcccgttg
     9001 aaaccggaag tggatatcga cgccctttcg cctgatcttg ctgcgctgag cgctatcagc
     9061 aaacagattg gctgcaatgg tttcttcccg ttccagatcc gcccaggcaa aaacgaaacc
     9121 gatggtcgta tgttctcgcc tgcgattggt attgtggaag atccggtgac tggcaatgcc
     9181 aatggcccga tgggtgcatg gttggtacat cacaacgtat tgccccacga tggcaacgtg
     9241 ttatgtgtta aaggccatca ggggcgcgca ttggggcgtg acggcatgat tgaagtgacg
     9301 gtgacaattc gtgataacca accggaaaaa gtcaccattt caggcacagc ggtgattttg
     9361 tttcatgccg aatgggcaat tgaactctga aaccgttgcc tgatgcgctt cgcctgtcag
     9421 gtctacaacc gagctaatgt atgccggata aggcgtttac gccgcatccg gcaacctgac
     9481 ataaattatt ttcccgcctc cgggtgcgta tcaaacgccg ccatcaccag cgctaactca
     9541 tccacggtaa agccatgttt cgcatcatcc acgcccagac caaactgttc ttgcatcaca
     9601 gcatataatg acgccacatc cggtagattt cgctgctcca ccgcgtgccc attttcataa
     9661 tgggtaaaat gaaaattggt cagcgttagc ttgccaccgt ccggcaaatg gcggcacatc
     9721 agcaaatgat ggcgaaaatg cgactgcggc cagtgcgccg accagaaatt gcccatcaca
     9781 taatcgcttt gttgctgctc gcagagatca aaacggtaca tcgactgcca gtgctgatga
     9841 tgattaaact gcaacaccca atcatcaccc tcctgcaaca accgataatc tccgtgtggc
     9901 gtggtctgca cgagatcgga aactaaacga atcggcgcgg ttagcgtctg cccaccgaaa
     9961 ccgacatcag caatccattt ttcctcttcc agttccacca acagcaaacg atgggtgcgc
    10021 ggcggtaatg ctggcggatt tgataacact acgcgcccta acaagctgcg aacgttaaac
    10081 tccagctcgc gtaacacccg ctcaaacacg ccattctgct caaaacagta accgccacga
    10141 cgggctatca ccagtttctc ttccagcgat tgatcatcaa gctgtatttc cctcggcagc
    10201 aaaacgtcga ggttttcaaa cggaatagtg caattgtgtt tcaggtgcaa tgcacgaagc
    10261 gtatcaatat tgaccgcagc agctcccgac cagttaatac gggcaaaata gtgattcaga
    10321 atgggcgtca tcaggcgttt cccttagtgg ttttctcaac ttataacctg gaatagacgc
    10381 cgcaccggct attttgtgcg atcgcggaca tgcatcacgt ttgcaaatcg ccaatcccgt
    10441 taatttcgtt acttattgcg ttacggacta cgcttaaaaa aaccttcttc gcgaggcgat
    10501 accatgagcc gattcatccc catagaatta caccatgcca gccgtctgtt aaatcatggc
    10561 ccaactgtca tgatcaccag tttcgatgag caatcccagc ggcgtaacat tatggcagca
    10621 gcctggtcaa tgccggtgga gtttgaaccg ccacgcgtgg cgattgtggt agataaatcg
    10681 acatggacca gggagttgat tgaacgtaac ggtaaatttg gcatcgttat cccgggcgtt
    10741 gcagcaacta actggacgtg ggcagtggga agcgtgtcgg gtcgtgaaga agataaattt
    10801 aattgctatg gcattccggt ggtgaaaggt ccggtgcttg gtttgccact tgttgaagag
    10861 aaatgcctgg cgtggatgga gtgccgcctg ctacctgcga cttctgcgca acaaaaatac
    10921 gacacgctgt ttggtgaggt ggtatcagca gcggcagacg cacgggtatt tgtcgaaggc
    10981 cgctggcagt ttgatgatga taagctcaat acgttgcatc atttaggtgc tggcacgttt
    11041 gttaccagcg gcaagcgagt tacggcgggt taagcattac atgctgtaac cgggtttctt
    11101 tatcaaagcc tccatttggg gcgcgatttc ggcatcccag atagcttgcc aagattctgg
    11161 ttgaatctgc tgtagagcaa tgcttatcga gctatcttta ctgttcagat ggcgaataat
    11221 aacgtcggta atatctgcag caagtgctgc tttttattgt tcgtccagtt cacgcggaaa
    11281 acatttaatg tcgatgtgcg gcatgattgt gctccttttg aaagatgtca gtaaaattaa
    11341 cacacattgt gaatgtcgtg tgtccggtaa tgcgttttta cgacaataat gccagggcaa
    11401 gattacgaaa gcct
//
LOCUS       DAZGOB010000146        11262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGOB010000146 DAZGOB010000000
VERSION     DAZGOB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.146"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..300
                     /gene="yoaC"
                     /locus_tag="ACHLIF_004256"
     CDS             <1..300
                     /gene="yoaC"
                     /locus_tag="ACHLIF_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO9608152.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(420..599)
                     /gene="yoaH"
                     /locus_tag="ACHLIF_004257"
     CDS             complement(420..599)
                     /gene="yoaH"
                     /locus_tag="ACHLIF_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO9608153.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            673..2034
                     /gene="pabB"
                     /locus_tag="ACHLIF_004258"
     CDS             673..2034
                     /gene="pabB"
                     /locus_tag="ACHLIF_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO9608154.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2038..2616
                     /gene="nudL"
                     /locus_tag="ACHLIF_004259"
     CDS             2038..2616
                     /gene="nudL"
                     /locus_tag="ACHLIF_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO9608155.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2800..4164
                     /gene="sdaA"
                     /locus_tag="ACHLIF_004260"
     CDS             2800..4164
                     /gene="sdaA"
                     /locus_tag="ACHLIF_004260"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO9608156.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4295..5893
                     /gene="pdeD"
                     /locus_tag="ACHLIF_004261"
     CDS             4295..5893
                     /gene="pdeD"
                     /locus_tag="ACHLIF_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9608157.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5897..7453)
                     /gene="yoaE"
                     /locus_tag="ACHLIF_004262"
     CDS             complement(5897..7453)
                     /gene="yoaE"
                     /locus_tag="ACHLIF_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO9608158.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7441..7599)
                     /gene="yoaL"
                     /locus_tag="ACHLIF_004263"
     CDS             complement(7441..7599)
                     /gene="yoaL"
                     /locus_tag="ACHLIF_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO9608159.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7916..8887
                     /gene="manX"
                     /locus_tag="ACHLIF_004264"
     CDS             7916..8887
                     /gene="manX"
                     /locus_tag="ACHLIF_004264"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO9608160.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAQAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8950..9750
                     /gene="manY"
                     /locus_tag="ACHLIF_004265"
     CDS             8950..9750
                     /gene="manY"
                     /locus_tag="ACHLIF_004265"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO9608161.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9763..10614
                     /gene="manZ"
                     /locus_tag="ACHLIF_004266"
     CDS             9763..10614
                     /gene="manZ"
                     /locus_tag="ACHLIF_004266"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO9608162.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10669..11127
                     /gene="yobD"
                     /locus_tag="ACHLIF_004267"
     CDS             10669..11127
                     /gene="yobD"
                     /locus_tag="ACHLIF_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO9608163.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2754 a         2840 c         2840 g         2828 t
ORIGIN      
        1 atgcctgctg taatagataa agccctggat ttcattggtg ccatggatgt atcagcgcca
       61 acaccaagtt cgatgaatga aagcacggcg aagggaatct ttaaatattt aaaagaactg
      121 ggagtacccg cgagtgccgc tgatattacg gcgcgagctg accaggaagg ctggaaccct
      181 gggttcacgg aaaaaatggt tggatgggca aaaaaaatgg agacaggtga acgctctgtg
      241 attaaaaatc ctgaatactt ttcaacatat atgcaggaag aactaaaagc actggtctga
      301 gttaaattta tatcagcata aatgggtcag gacgctttta atcacatata aaaagcgtcc
      361 tttttctcac aatcaaatga tcatcggctt ctataacgaa atcgatacgg attagtgtgt
      421 tactcgtctt catcctcaaa acgcgccacg attcgctcgc cggagtggtt ggcacgcagc
      481 tcttctgcca ccagcgcgat tgcctggccg ctgctcatcc cttgtgccat cagttcctgg
      541 atccgctcga cagctttttg ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt
      601 gttaactcct gctaaattgt tggcgctaat tatttcatgc tacccggcac atagccagta
      661 gagtcaggac tgatgaagac gttatctccc gctgtgatta ctttaccctg gcgtcaggac
      721 gccgctgaat tttatttctc ccgcttaagc cacctgccgt gggcgatgct tttacactcc
      781 ggctatgccg atcatccgta tagccgcttt gatattgtgg tcgccgatcc gatttgcact
      841 ttaaccactt tcggtaaaga aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact
      901 gatgacccgc tacaggtgct ccagcaggtg ctggatcgcg cagacattcg cccaacgcat
      961 aacgaagatt tgccatttca gggcggcgca ctggggttgt ttggctacga tctgggccgc
     1021 cgttttgagt cactgccaga aattgcggaa caagatatcg ttctgccgga tatggcagtg
     1081 ggtatctacg attgggcgct cattgtcgac caccagcgtc atacagtttc tttgctgagt
     1141 cataatgatg tcaatgcccg tcgggcctgg ctggaaagcc agcaattctc gccgcaggaa
     1201 gatttcacgc tcacttccga ctggcaatcc aatatgaccc gcgagcagta cggcgaaaaa
     1261 tttcgccagg tacaggaata tctgcacagc ggtgattgct atcaggtgaa tctcgcccag
     1321 cgttttcatg cgacctattc tggcgatgaa tggcaggcat tccttcagct taatcaggcc
     1381 aaccgcgcgc catttagcgc ttttttacgt cttgaacagg gtgcaatttt aagcctttcg
     1441 ccagagcggt ttattctttg tgataatagt gaaatccaga cccgcccgat taaaggcacg
     1501 ctaccacgcc tgcccgatcc tcaggaagat agcaaacaag cagaaaaact ggcgaactca
     1561 gcgaaagatc gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt
     1621 gttgccgtag ccggttcggt aaaagtacca gagctcttcg tggtggaacc cttccctgcc
     1681 gtgcatcatc tggtcagcac tataacggcg cgactaccag aacagttaca cgccagcgat
     1741 ctgctgcgcg cagcttttcc tggtggctca ataaccgggg ctccgaaagt acgggctatg
     1801 gaaattatcg acgaactgga accgcagcga cgtaatgcct ggtgcggcag cattggctat
     1861 ttgagctttt gcggcaacat ggataccagc attactatcc gcacgctgac tgccattaac
     1921 ggacaaatat actgctctgc gggcggtgga attgtcgccg atagccagga agaagcggaa
     1981 tatcaggaaa cttttgataa agttaataag atattacgcc aactggagaa gtaagacgtg
     2041 gaataccgta gcctgacgct tgatgatttt ttatctcgct ttcaactttt gcgcccgcaa
     2101 attaaccggg aaaccctaaa tcatcgtcag gctgccgtgt taatccccat cgtccgtcga
     2161 ccgcaaccgg ggttgttgct gactcagcgt tcaattcatc tgcgtaaaca cgctggacaa
     2221 gtggcattcc ctggaggtgc agtcgatgac acggacgcat cagttatcgc cgccgcgctg
     2281 cgcgaagctg aagaagaggt cgctataccg ccttccgccg ttgaagttat cggcgtgctg
     2341 ccgcccgtcg atagcgtaac tggctatcag gtaaccccag tggtcggcat tatcccgccc
     2401 gatctgccgt atcgcgccag tgaagatgaa gtctcggcgg tgtttgaaat gccgctcgcc
     2461 caggcattac atctgggtcg ttatcaccct ttagatatct accgccgtgg cgattcacat
     2521 cgggtatggc tgtcctggta cgaacagtat tttgtatggg gaatgaccgc aggcataatt
     2581 cgtgagctgg cgctgcaaat tggtgtgaaa ccctgactat acttatcttt acatctacaa
     2641 aacactactt gagacaatca tcgcaatatt agttaaatcg cggtttttga ttagtttaat
     2701 tcatgtgaat agttaagcca gtcgccgcgt tccctcttac actatgcgct gttattagtt
     2761 cgttactgga agtccagtca ccttgtcagg agtattatcg tgattagtct attcgacatg
     2821 tttaaggtgg ggattggtcc ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa
     2881 cagttcgtcg atgatctggt cgaaaaaggc ttactggata gcgttactcg cgttgccgtg
     2941 gacgtttatg gttcactgtc gctgacgggt aaaggccacc acaccgatat cgccattatt
     3001 atgggtcttg caggtaacga acctgccacc gtggatatcg acagtattcc cggttttatt
     3061 cgcgacgtag aagagcgcga acgtctgctg ctggcacagg gacggcatga agtggatttc
     3121 ccgcgcgaca acgggatgcg ttttcataac ggcaacctgc cgctgcatga aaacggtatg
     3181 caaatccacg cctataacgg cgatgaagtc gtctacagca aaacttatta ttccatcggc
     3241 ggcggtttta tcgtcgatga agaacacttt ggtcaggatg ctgccaacga agtgagcgtg
     3301 ccgtatccgt tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac cggctattcg
     3361 ctgtctggtc tcgctatgca gaacgaactg gcgctgcaca gcaagaaaga gatcgacgag
     3421 tatttcgcgc atgtctggca aaccatgcag gcatgtatcg atcgcgggat gaacaccgaa
     3481 ggtgtactgc caggcccgct gcgcgtgcca cgtcgtgcgt ctgccctgcg ccggatgtta
     3541 gtttccagcg ataaactgtc taacgatccg atgaatgtca ttgactgggt aaacatgttt
     3601 gcgctggcag ttaacgaaga aaacgccgcc ggtggtcgtg tggtaactgc gccaaccaac
     3661 ggtgcctgcg gtatcgttcc ggcagtgctg gcttactatg accactttat tgaatcggtc
     3721 agcccggaca tctatacccg ttactttatg gcagcgggcg cgattggtgc attgtataaa
     3781 atgaacgcct ctatttccgg tgcggaagtt ggttgccagg gcgaagtggg tgttgcctgt
     3841 tcaatggctg ctgcgggtct tgcagaactg ctgggcggta gcccggaaca ggtttgcgtg
     3901 gcggcggaaa ttggcatgga acacaacctt ggtttaacct gcgacccggt tgcagggcag
     3961 gttcaggtgc cgtgcattga gcgtaatgcc attgcctctg tgaaggcgat taacgccgca
     4021 cggatggctc tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg
     4081 atgtacgaaa ccggtaagga catgaacgcc aaataccgcg aaacctcacg cggtggtctg
     4141 gcaatcaaag tccagtgtga ctaatacttc ttactcgccc atctgcaacg gatgggcgaa
     4201 tttatacccg ctttctcgtc tgctgtaata ttccccacta cacttccact gttgcgtcag
     4261 gcgtttgtcg ccatacgctt acagggtggc ccgcatgcaa aaagcacaac ggatcattaa
     4321 aacctatcgc cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta cgctcgcttc
     4381 gaccctgagc gtgcgattta tttcacagcg taacttaaat caacaacggg tagtacaatt
     4441 cgccaatcac gctgtagagg aattagataa agtactgctt cccctacagg caggtagcga
     4501 agtcttgctt ccgctgattg gtctgccctg ctctgtcgcc catttgccat tacgtaaaca
     4561 ggcggcaaaa ctccaaactg tgcgatccat tggcctggtg caagacggca cactctattg
     4621 ctccagcatt tttggttatc gcaatgtgcc cgtcgtggac attctggctg aacttcctgc
     4681 accgcaacca cttttacgcc tgacgatcga ccgtgccctg attaaaggca gtccggtttt
     4741 gattcaatgg acgcctgcag cgggcagtag caatgctggg gtcatggaga tgattaacat
     4801 cgacttactg acggcaatgc tccttgagcc acaactgccg caaatcagta gcgccagcct
     4861 gacggtggac aaacggcatt tgctctatgg taatgggctg gtagattccc ttccgcaacc
     4921 tgaagacaat gaaaactatc aggtttcttc gcaacgcttt ccttttacca ttaacgttaa
     4981 tggtccgggg gctacggcgc tggcatggca ctatcttcca acacaattac cgctggcggt
     5041 gctgctaagt ttactggtgg gctacatcgc ctggctggcg accgcttacc ggatgagctt
     5101 ttcccgcgaa atcaatctgg gcctggcgca acatgagttc gaattgttct gtcagccttt
     5161 gcttaatgcg cgcagccagc aatgtattgg tgtagagatt ttgctgcgct ggaataatcc
     5221 gcgtcagggc tggatttcac cggatgtgtt tattcctatc gcggaagaac atcatttaat
     5281 tgtgccactg acccgctatg tgatggcaga aaccattcgt cagcgccatg ttttcccgat
     5341 gagtagtcag tttcatgttg gcattaacgt cgcacccagc cattttcgcc gtggtgtgct
     5401 gataaaagat ctcaatcagt actggtttag cgctcacccg attcagcaac tgatcctcga
     5461 aatcaccgaa cgcgatgcct tactggatgt tgattatcgg attgcccgcg agctacatcg
     5521 taaaaacgtc aaactggcga ttgatgactt cggcaccggc aacagttcat tttcctggct
     5581 tgaaacatta cgtcctgacg tgctgaaaat tgataagtca tttaccgcag ctataggttc
     5641 tgacgcggtt aactcgacgg tgaccgatat catcatcgcg ctggggcaaa gactgaatat
     5701 tgaactggtg gcggagggcg tggaaacgca agaacaggcg aagtatttgc gccgtcatgg
     5761 cgtgcatatt ttgcaagggt atttgtacgc acagccgatg ccgctacgtg attttcccaa
     5821 atggctggcg ggcagccaac cgccgcccgc ccggcataat ggacatatca cgcccattat
     5881 gccgttacgt taatgcttac tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa
     5941 ccagatcaac acgataatca ttggcttcaa tgatggtgat atgcagtggc cctacatcaa
     6001 tcacatcgcc cacacgggga atgtgaccat ttgccgagat cacgaggccc gcgaccgtcg
     6061 cgatatcatc gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat
     6121 ctgtaccgcc ttttaccagc cagccgtcac catcagtaat gatttccggc gtttcgtcag
     6181 cgtccgggaa ttcacccgca atggcttcca gcacatccag cggcgtgacc agaccttgta
     6241 ccacaccaaa ctcgttggtc acgataacaa agctcccgcg agcacgacgc agcacgccca
     6301 acaggttgat cggatcgagg gtttccggga cgataatcgc cggagacgcc gaagcaatcg
     6361 ccgccacatc aacgccctct tccagcgcca ccagcagttc tttagcacgt acaataccga
     6421 tgatttcatc cagttcaccg cgacataccg ggaacagact gtgcggtgaa gagagcagtt
     6481 gctcgcggat ttcatcgacc ccgagattag cgtcaaccca gcttatttca ccgcgcggcg
     6541 tcatgatccc gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc atgtaacgtt
     6601 cttcttcggc aaatgcacct tccgggatcg gcatcggcat cgggttatcg gcatcgtgct
     6661 gaacattggc ctgacgtttc ccgcccatca aacgcaggat ggcatcggca gtacgcgctc
     6721 gcagcggcaa agtcgactgg tggcgaataa agttgcgacg cgcaatctgg ttaaacactt
     6781 cgatgatgat cgagaagcca atcgcggcat acaggtaacc tttcggaatg tggaaaccga
     6841 aaccttctgc caccagactc agaccaatca ttaacaggaa gctcagacag agcaccacca
     6901 ccgtggggtg ctggttaacg aatcgcgtca gcggtttgga tgccagcaac ataaccgcca
     6961 tcgcaatcac taccgccgcc atcatcaccg gcagatggtt aaccatccct actgcagtaa
     7021 ttaccgcatc caacgagaag acggcgtcaa ggatgacaat ctgtgtgacg accacccaga
     7081 aactggcgta gcctttaccg tggccggaat catgatcgcg gttttccagc cgttcatgca
     7141 gttcggttgt tgctttgaac agcaagaata tccccccgaa caacataatc aggtcgcgtc
     7201 cggagaagga gaaatccatg acggtaaata gcggtttggt cagcgtgacc atccatgaaa
     7261 tcagcgacag cagccccaga cgcataatca gcgccagtga taaccccagc aaacgcgctt
     7321 tatcgcgttg ttttggcggc agtttgtcag caagaatggc gatgaagacc aggttatcga
     7381 tacccagcac aatttcgaga acaacaagcg tgagtagccc cgcccaaatt gaggggtcca
     7441 ttaagaattc catgacaagc tcctgcttaa ggaatagcta ttcgacgcca gaaataatgc
     7501 aggcgtaacg acaaaatgca aacgaaaggt gcggcataga gagccagaaa ggtaggcgtt
     7561 aaaaggcctg atgctgaaat gacgtcggtg acgatccata ctgcgggcta ctgccctata
     7621 ctccatggtt gttaaacggg agttaaacat atcagagacg cctctgattt ggcaaagatt
     7681 taccttcctt tgcaaacgaa tgtgacaagg atattttacc tttcgaaatt tctgctaatc
     7741 gaaagttaaa ttacggatct tcatcacata aaataatttt ttcgatatct aaaataaatc
     7801 gcgaaacgca ggggtttttg gttgtagccc ttatctgaat cgattcgatt gtggacgacg
     7861 attcaaaaat acatctggca cgttgaggtg ttaacgataa taaaggaggt agcaagtgac
     7921 cattgctatt gttataggca cacatggttg ggctgcagag cagttgctta aaacggcaga
     7981 aatgctgtta ggcgagcagg aaaacgtcgg ctggatcgat ttcgttccag gtgaaaatgc
     8041 cgaaacgctg attgaaaagt acaacgctca gttggcaaaa ctcgacacca ctaaaggcgt
     8101 gctgtttctc gttgatacat ggggaggcag cccgttcaat gctgccagcc gcattgtcgt
     8161 cgacaaagag cattatgaag tcattgcagg cgttaacatt ccaatgctcg tggaaacgtt
     8221 aatggcccgt gatgatgacc caagctttga tgaactggtg gcactggcag tagaaacagg
     8281 ccgtgaaggc gtgaaagctc tgaaagccaa accggttgaa aaagccgcgc aagcacccgc
     8341 tgccgcagca ccaaaagcgg ctccaactcc ggcaaaacca atggggccaa acgactacat
     8401 ggttattggc cttgcgcgta tcgacgaccg tctgattcac ggtcaggtcg ccacccgctg
     8461 gaccaaagaa accaatgtct cccgtattat tgttgttagt gatgaagtgg ctgcggatac
     8521 cgttcgtaag acactgctca cccaggttgc acctccgggc gtaacagcac acgtagttga
     8581 tgttgccaaa atgattcgcg tctacaacaa cccgaaatat gctggcgaac gcgtaatgct
     8641 gttatttacc aacccaacag atgtagagcg tctcgttgaa ggcggcgtga aaatcacctc
     8701 tgttaacgtc ggtggtatgg cattccgtca gggtaaaacc caggtgaata acgcggtttc
     8761 ggttgatgaa aaagatatcg aggcgttcaa gaaactgaat gcgcgcggta ttgagctgga
     8821 agtccgtaag gtttccaccg atccgaaact gaaaatgatg gatctgatca gcaaaatcga
     8881 taagtaacgt attgtgttga ttatcactca gttttcacac ttaagtctta cgtaaacagg
     8941 agaagtacaa tggagattac cactcttcaa attgtgctgg tatttatcgt agcctgtatc
     9001 gcaggtatgg gatcaatcct cgatgaattt cagtttcacc gtccgctaat cgcgtgtacc
     9061 ctggtgggta tcgttcttgg ggatatgaaa accggtatta ttatcggtgg tacgctggaa
     9121 atgatcgcgc tgggctggat gaacatcggt gctgcagttg cgcctgacgc cgctctggct
     9181 tctatcattt ctaccattct ggttatcgca ggtcatcaga gcattggtgc aggtatcgca
     9241 ctggcaatcc ctctggccgc tgcgggccag gtactgacca tcatcgttcg tactattacc
     9301 gttgctttcc agcacgctgc ggataaggct gctgataacg gcaacctgac agcgatttcc
     9361 tggatccacg tttcttctct gttcctgcaa gcaatgcgtg tggctattcc ggccgtcatc
     9421 gttgcgctgt ctgttggtac cagcgaagta cagaacatgc tgaatgcgat tccggaagtg
     9481 gtgaccaatg gtctgaatat cgccggtggc atgatcgtgg tggttggtta tgcgatggtt
     9541 atcaacatga tgcgtgctgg ctacctgatg ccgttcttct acctcggctt cgtaaccgca
     9601 gcattcacca actttaacct ggttgctctg ggtgtgattg gtactgttat ggcagtgctc
     9661 tacatccaac ttagcccgaa atacaaccgc gtagccggtg cgcctgctca ggcagctggt
     9721 aacaacgatc tcgataacga actggactaa caggtgagcg aaatggttga tacaactcaa
     9781 actaccaccg agaaaaaact cactcaaagt gatattcgtg gcgtcttcct gcgttctaac
     9841 ctcttccagg gttcatggaa cttcgaacgt atgcaggcac tgggtttctg cttctctatg
     9901 gtaccggcaa ttcgtcgcct ctaccctgag aacaacgaag ctcgtaaaca agctattcgc
     9961 cgtcacctgg agttctttaa cacccagccg ttcgtggctg cgccgattct cggcgtaacc
    10021 ctggcgctgg aagaacagcg tgctaatggc gcagagatcg acgacggtgc tatcaacggt
    10081 atcaaagtcg gtttgatggg gccgctggct ggtgtaggcg acccgatctt ctggggaacc
    10141 gtacgtccgg tatttgcagc actgggtgcc ggtatcgcga tgagcggcag cctgttaggt
    10201 ccgctgctgt tcttcatcct gtttaacctg gtgcgtctgg caacccgtta ctacggcgta
    10261 gcgtatggtt actccaaagg tatcgatatc gttaaagata tgggtggtgg cttcctgcaa
    10321 aaactgacgg aaggggcgtc tatcctcggc ctgtttgtca tgggggcatt ggttaacaag
    10381 tggacacatg tcaacatccc gctggttgtc tctcgcatta ctgaccagac gggcaaagaa
    10441 cacgttacta ctgtccagac tattctggac cagttaatgc caggcctggt accactgctg
    10501 ctgacctttg cttgtatgtg gctactgcgc aaaaaagtta acccgctgtg gatcatcgtt
    10561 ggcttcttcg tcatcggtat cgctggttac gcttgcggcc tgctgggact gtaagactgt
    10621 tgtacactac cggggccttt tggccccgtt tttttatctg gaggattaat gacaatcacg
    10681 gacctggtac tgattctttt catcgcagca ctcctggcct tcgcgatcta cgatcagttc
    10741 atcatgcccc gccgtaacgg ccccaccctg ctggcaattc ctttgctccg gcgtggtcgc
    10801 atcgatagcg ttatcttcgt cggattgatt gtcattctta tctataacaa cgtcacgaat
    10861 catggtgcgt taataacgac atggttatta agcgcactgg ctctgatggg tttttatata
    10921 ttctggatcc gcgttccgaa gatcatcttt aaacaaaaag gttttttctt cgccaatgtc
    10981 tggattgaat atagccgaat caaagcgatg aacttgtcgg aagatggcgt gttggtgatg
    11041 caattagaac agcgtcggct gttaatccgc gttcgaaata tcgacgatct ggaaaaaatt
    11101 tataagcttc tcgtttcaac tcaataagtt atgaatttag ccaaagctat gtttagtgta
    11161 tttttaataa tcagacatag cttaggctat attacctctt cccttatttg ttatttattt
    11221 taacgtttca ttgatatata aatctaaatg taaatcgtta tc
//
LOCUS       DAZGOB010000147        11053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGOB010000147 DAZGOB010000000
VERSION     DAZGOB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.147"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            177..800
                     /gene="nfeR"
                     /locus_tag="ACHLIF_004268"
                     /gene_synonym="yqjI"
     CDS             177..800
                     /gene="nfeR"
                     /locus_tag="ACHLIF_004268"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO9608164.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(954..2474)
                     /gene="aer"
                     /locus_tag="ACHLIF_004269"
     CDS             complement(954..2474)
                     /gene="aer"
                     /locus_tag="ACHLIF_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO9608165.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2892..4271
                     /gene="ygjG"
                     /locus_tag="ACHLIF_004270"
     CDS             2892..4271
                     /gene="ygjG"
                     /locus_tag="ACHLIF_004270"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO9608166.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4313..4645)
                     /locus_tag="ACHLIF_004271"
     CDS             complement(4313..4645)
                     /locus_tag="ACHLIF_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO9608167.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            4864..5847
                     /gene="ebgR"
                     /locus_tag="ACHLIF_004272"
     CDS             4864..5847
                     /gene="ebgR"
                     /locus_tag="ACHLIF_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO9608168.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            6031..9123
                     /gene="ebgA"
                     /locus_tag="ACHLIF_004273"
     CDS             6031..9123
                     /gene="ebgA"
                     /locus_tag="ACHLIF_004273"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO9608169.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQENEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            9120..9569
                     /locus_tag="ACHLIF_004274"
     CDS             9120..9569
                     /locus_tag="ACHLIF_004274"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO9608170.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9632..>11053
                     /locus_tag="ACHLIF_004275"
     CDS             9632..>11053
                     /locus_tag="ACHLIF_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9608171.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTVGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPAS"
BASE COUNT         2640 a         2977 c         2800 g         2636 t
ORIGIN      
        1 atatctgaag tatgtgaagg cattcagtgc catagcaatg ctgccggatg cggcgtgaac
       61 gtcttatccg gcctggatgt gaggtgcttc cacgtttaaa aatcattttt gtacttgcag
      121 taatgctcac atcagatata aattagatat atctaatcaa gcaaaaggag gctgatatga
      181 gccatcatca cgaagggtgt tgtaaacatg aaggccagcc acgccatgag ggctgctgca
      241 aaggtgagaa gtcagaacac gagcactgcg gacacggtca ccagcatgaa cacggtcaat
      301 gctgcggtgg tcgccacggt cgcggcggcg gtcgtcggca acgtttcttt ggtcacggtg
      361 aattgcgtct ggtgattctg gatattctct cgcgcgatga cagccacggt tacgaattga
      421 ttaaagcgat tgagaaccta acccagggga attacacccc aagcccgggc gtcatctacc
      481 caacgctgga ttttctgcag gagcagtcgc tgattaccat ccgcgaagag gaaggtggta
      541 agaagcagat tgcgctgacc gaacaaggcg cgcagtggct ggaagaaaac cgcgaacagg
      601 tggagatgat tgaagaacgc atcaaagcgc gttgcgttgg cgcggcgctg cgccagaacc
      661 cgcaaatgaa gcgggcgctg gataatttta aagcggtgct ggatttacac gtcaaccaga
      721 gcgatatcag tgatgcacaa ataaaaaaga tcattgcggt gatcgaccgc gccgcttttg
      781 atattacgca actggattaa tcgccgcatc cgccagtggc gcggtgcaat tgccggatgc
      841 gacgcttgac gcgccttatc cggcctacac ccgctacaca ccccgcaggc ctgataagat
      901 gcgccagcat cgcatcaggc attgtgctcc aaccgccgga tccggcatac cgattaatgc
      961 agcaccgtca ccgcgtcttc cagtcggctg gcgcggtgtt tcaccatcgc cgacacctgc
     1021 gcactctctt ccaccagctc ggcatttttc tgggtgatca ggttaagctc atccactgca
     1081 cgggtcaggc tggaaagccc atcggcctgt tccagcgttg aatggctaat ctgggcgatc
     1141 aactgggtga cgtttttcac ctgtgccaca atatcttcca tcgtccgtcc ggcggcgtgt
     1201 acctgctgcg aaccggattg caccttatca gcactggcat caatcagctt gcgaatatcg
     1261 ttggcagcat tagcgctgcg gctggctaaa tgacgcactt cccctgccac cactgcaaaa
     1321 cctttgccct gttcgccggc acgcgccgct tccaccgccg cattcagggc cagaatattg
     1381 gtctgaaacg caatatcgtt aatcagcgaa gtaatggtgc caatgcgctg ggtactgtcg
     1441 gcgatatcgt ccattgtctt gatcaccgtg gtcatcgcct ccccaccctg cactgccgca
     1501 ttactggcgg tgatagaaag tttatcggca gccgacgccg tggcagagtt ctgtttcacc
     1561 gacgccgcca tttggttcat ggtcgccacc gtttgctgaa cgttatcaac tgtctgctgg
     1621 gtatgttcgt tcagttcatc agtgcctttc gccagcgtct cactgccatt tctgacactg
     1681 gacacctggc ttgagacatc gttaattagc caacggcaca tcaggccaag ctgccctacc
     1741 gcgcgtaatg tcagccccag ctcatcgctg cgattcaggt gctcaacact attacgctct
     1801 ccggtcgcca ccttcagcgc ctgacgggcg acattttcta tcgggcgcac aatctgccat
     1861 tcaaaacaag cgcttgccaa caataccact aacacacaga ggaaatacgt caccaccggg
     1921 gcagcaacaa accaaagcat ggccgccagc aagataaaca tcagggtcat cactccacgc
     1981 gcccgccagc gaagcggtaa tgaaggcagt ttacccagcc agcctttacg caccaccagg
     2041 cctttatgaa tacgcttact ggtacgtccg gcgttcagcg ctttgtacag cggctccacc
     2101 gccgcaatct cttcatccgt cgcccgggta cgaatcgaca tatagccact gatttttccc
     2161 tcgcgcacca tcggtaccgc attggcccgc acccaataat ggtcgccatt tttgcggcga
     2221 tttttcacga tgccgctcca gggctcccct tttttcaggg tgaaccacat atccgcaaac
     2281 gccgcttttg gcatatccgg gtgacgcacc atgttgtgcg gctgcccttg taactcttgc
     2341 aaggtaaagc cgctcacctg cacaaaagtg tcattagcat gagtgatata gctttgcaga
     2401 tcggtagtgg acatcagagt ggtatcgtcc gccagcgggg tattttgctg ggtgacatac
     2461 ggatgagaag acatgttcgc gtcctgtgca ggttatatgg ttgttaactt cttgtcagag
     2521 tttatgtcgg ccccgctgcg gttatcttta accgattaat ttgatttaga tcgcaatttg
     2581 cgattaaagc acaaatctaa ttctttgatt taaaatactt tcactctgtt actatacgaa
     2641 aacgttaatt atcttgccca aaaatcaggc aattattgcc ctgaaaacgt gcatttgcgc
     2701 agcaatcatc aaatccatac ccgacaaaaa ccgtgcaaaa taacaacaaa tgttaacaga
     2761 tagcattaaa tattgcacaa atgataaccg aatttgtgtt tatcccgatt ttcgcgatcg
     2821 cagccggagt ggcgcaatcc ctgcaatact taaatcggta tcatgtgata cgcgagcctc
     2881 cggagcatat tttgaacagg ttaccttcga gcgcatcggc tttagcgtgc agcgcccacg
     2941 ccctgaatct cattgagaag cgaacgctgg atcatgagga gatgaaagca cttaaccgag
     3001 aggtgattga atacttcaaa gagcatgtca atccggggtt tttagagtat cgcaaatctg
     3061 ttaccgccgg cggggattac ggagccgtag agtggcaagc gggaggttta aatacgcttg
     3121 tcgacaccca gggacaggag tttatcgact gcctgggagg ttttggaatt ttcaacgtgg
     3181 ggcaccgtaa tccagttgtg gtttccgccg tacagaatca acttgcgaaa caaccgctgc
     3241 acagccagga gctgctcgat ccgttacggg cgatgttggc gaaaaccctt gctgcgctaa
     3301 cgcccggtaa actgaaatac agcttcttct gtaatagcgg caccgagtcc gttgaagcag
     3361 cgctgaagct ggcgaaagct taccagtcac cgcgcggcaa gtttactttt attgccacca
     3421 gcggcgcgtt ccacggtaaa tcacttggcg cgctgtcggc cacggcgaaa tcgaccttcc
     3481 gcaaaccgtt tatgccactg ctgccgggct tccgtcatgt gccgtttggc aatatcgaag
     3541 ccatgcgcac ggctcttaac gagtgcaaaa aaaccggtga tgatgtggct gcggtgatcc
     3601 tcgaaccgat tcagggtgaa ggtggcgtaa ttctgccgcc gccgggctat ctcaccgccg
     3661 tacgtaagct atgcgatgag ttcggcgcac tgatgatcct cgatgaagta caaacgggca
     3721 tggggcgcac gggcaagatg ttcgcctgcg agcatgagaa cgtacagccg gatatcctct
     3781 gccttgccaa agcgctcggc ggcggcgtga tgccgattgg cgcgaccatc gccactgaag
     3841 aggtgttttc agttctgttc gacaacccat tcctgcatac caccaccttt ggcggcaacc
     3901 cgctggcctg tgcggcggcg ctggcgacca tcaatgtgtt gctggagcag aacttaccgg
     3961 ctcaggctga gcaaaaaggc gatatgttgc tggacggttt ccgtcaactg gcgcgggaat
     4021 atcccgatct ggtacaggaa gcgcgtggta aagggatgtt gatggcaatt gagtttgttg
     4081 ataacgaaat cggctataac tttgccagcg agatgttccg ccagcgcgta ctggtggccg
     4141 gaacgctcaa taacgccaaa acgatccgca ttgaaccgcc actgacactg accattgaac
     4201 agtgtgaact ggtgatcaaa gcggcgcgta aggcgctggc ggccatgcga gtaagtgtcg
     4261 aagaagcgta atatcaaatc ggatggcgat acgacgtcgc catccgatct ttttatacga
     4321 cgcgtacgcc cgcaggcatc atccgctccg gcgttaacaa cacgctttca ctgccgtcat
     4381 cggtttccgc acacaacagc atgcattccg acgtttcacc acgcattttc gctttttgca
     4441 gattgcataa gaccaccacc gttttcccca tcagctcttc ttcgctgtag tacggcacca
     4501 ggctggtcac ggtttgcagt gttttttccc ccacatcaac ctgtacgatg tacagcttgt
     4561 cggcgttttc atggcgtttc acttccacaa tctttccgac gcgcatttcc agacgtgcaa
     4621 aatcagcgta agccacggtt tccattgttt cctcccttcg ggtaaaattt cactaaacta
     4681 tagcaaaagt ttttctcaat cctgtaggct aaaaatggag aatgcaggcg tgatcacatt
     4741 cgtgagccgc tgtgttaccg ttacagcgtc aaagaaacgc gctttattta ctgaaaacag
     4801 gtgacccgat aagcacttcc tctacaatgg gggcgcacat cagggaaagt aaaaaaggta
     4861 aacatggcaa cactaaaaga catcgcaatc gaagctggcg tatccctggc gacagtatcc
     4921 agggtcttaa atgacgatcc gacattgaat gtgaaagaag agacgaaaca tcgcattctc
     4981 gagatcgccg aaaagctgga gtacaagacc agtagtgccc gtaaactcca gacaggtgca
     5041 gtcaaccaac accatattct ggctatctac agctaccagc aggagctgga gatcaacgat
     5101 ccttactatc tggcgatccg ccacggcatt gaaacccagt gcgaaaagct gggcatcgag
     5161 ctcaccaact gttatgaaca cagcggctta ccagacatta aaaacgtcac cggtatttta
     5221 attgtcggca aacccacgcc cgccctgcgc gccgctgcca gcgcgttgac cgacaatatc
     5281 tgttttatcg actttcacga acccggcagc ggttacgatg cggtggatat cgatctggca
     5341 cgcatcagta aagaaatcat cgacttctat atcaaccagg gcgttaatcg tattggtttt
     5401 attggcggtg aagatgagcc tggcaaggcg gatattcgtg aggtcgcctt tgcggaatat
     5461 ggccgactga aacaagtggt acgcgaagag gatatctggc gcggcggttt ttccagttcg
     5521 tcgggttatg aactggcaaa acaaatgctg gcgcgggaag actatccgaa ggcactgttt
     5581 gttgcttccg attccattgc tatcggcgta ctgcgggcaa ttcatgaacg aggcctgaac
     5641 atcccacagg atatttcgct tatcagcgtt aacgatatcc ccaccgcgcg atttaccttt
     5701 ccgccgctct ccaccgtgcg catccattcc gagatgatgg gaagtcaggg cgttaacctg
     5761 gtgtatgaaa aagcccgcga tggtcgcgcg ctgccgctgt tagtcttcgt tcccagcaaa
     5821 ttaaaactgc gcggcacgac ccgttaaatc cccttacaca ctgtccggca atcgtttttg
     5881 ccggacagtg ctgccgttta ttttcgtgat ccagttaaag taaatgcatt cacctgctac
     5941 tttttagtaa aaattttact aaactcccca gcaattacac aaactaccat caccatgaat
     6001 ggttccgatt tctctctacc gggaggccct atgaatcgct gggaaaacat tcagctcacc
     6061 cacgaaaacc gacttgcgcc gcgtgcgtac tttttttcat atgattctgt tgcgcaagcg
     6121 cgtacctttg cccgcgaaac cagcagcctg tttctgccct taagcggtca gtggaatttc
     6181 cacttttttg accatccgct gcaagtacca gaagccttca cctctgagtt aatggctgac
     6241 tgggggcata ttaccgtccc cgccatgtgg caaatggaag gtcacggcaa actgcaatat
     6301 accgacgaag gttttccgtt ccccatcgat gtgccgtttg tccccagcga taacccaacc
     6361 ggtgcctatc aacgtatttt caccctcagc gacggctggc agggtaaaca gacgctgatt
     6421 aaatttgacg gcgtcgaaac ctattttgaa gtctatgtta acggtcagta tgtgggtttc
     6481 agcaagggca gtcgcctgac cgcagagttt gacatcagcg cgatggttaa aaccggcgac
     6541 aacctgttgt gtgtgcgcgt gatgcagtgg gcggactcta cctacgtgga agaccaggat
     6601 atgtggtggt cagcggggat cttccgcgat gtttatctgg tcggaaaaca cctaacgcat
     6661 attaacgatt tcactgtgcg taccgacttt gacgaagcct attgcgatgc cacgctttcc
     6721 tgcgaagtgg tgctggaaaa tctcgccgcc tcccctgtcg tcacgacgct ggaatatacc
     6781 ttgtttgatg gcgaacgcgt ggtgcacagc agcgccattg atcatttggc aattgaaaaa
     6841 ctgaccagcg ccagctttgc ttttactgtc gaacagccgc agcaatggtc agcagaatcc
     6901 ccttatcttt accatctggt catgacgctg aaagacgcca acggcaacgt tctggaagtg
     6961 gtgccacaac gcgttggctt ccgtgatatc aaagtgcgcg acggtctgtt ctggatcaat
     7021 aaccgttatg tgatgctgca cggcgtcaac cgtcacgaca acgatcatcg caaaggccgc
     7081 gccgttggaa tggatcgcgt cgagaaagat ctccagttga tgaagcagca caatatcaac
     7141 tccgtgcgta ccgctcacta cccgaacgat ccgcgttttt acgaactgtg tgatatctac
     7201 ggcttgtttg tgatggcgga aaccgacgtc gaatcgcacg gctttgctaa tgtcggcgat
     7261 atcagccgta ttaccgacga tccgcagtgg gaaaaagtct acgtcgagcg cattgttcgc
     7321 catattcacg cgcagaaaaa ccatccgtcg atcatcatct ggtcgctggg caatgaatcc
     7381 ggctatggct gtaacatccg cgcgatgtac cacgcagcga aggcgctgga tgacacgcga
     7441 ctggtgcatt acgaagaaga tcgcgatgct gaagtggtcg atattatttc caccatgtac
     7501 acccgcgtgc cgctgatgaa tgagtttggt gaatacccgc atccgaagcc gcgcatcatc
     7561 tgtgaatatg ctcatgcgat ggggaacgga ccaggcgggc tgacggagta ccagaacgtc
     7621 ttctataagc acgattgtat tcagggacat tatgtttggg agtggtgcga ccacgggatc
     7681 caggcgcagg atgacaacgg caacgtctgg tataaattcg gcggcgacta cggcgactat
     7741 cccaacaact ataacttctg tcttgatggt ttgatctatt ccgatcagac gccgggaccg
     7801 ggcctgaaag agtacaaaca ggttatcgcg ccggtaaaaa tccacgcgct ggatctgact
     7861 cgcggcgagc tgaaagtcga aaataaactg tggtttacca cgcttgatga ctacaccctg
     7921 cacgcagagg tgcgcgtcga aggtgaaacg ctcgcgacgc agcagattaa actgcgcgac
     7981 gttgcgccga acagcgaagc ccccttgcag atcacgctgc cgcagctgga cgcccgcgaa
     8041 gcgttcctca acattacggt gaccaaagat tcccgcaccc gctacagcga agccgggcat
     8101 tctatcgcca cttatcagtt cccgctgaag gaaaacaccg cgcaaccagt gcctttcgca
     8161 ccaaataatg cgcgtccgct gacgctggaa gacgatcgtt tgagctgcac cgttcgcggc
     8221 tacaacttcg cgatcacctt ctcaaaaatg agtggcaaac cgacatcctg gcaggtgaat
     8281 ggcgagtcgc tgctgacccg cgagccaaag atcaacttct tcaagccaat gatcgacaac
     8341 cacaagcagg agaacgaagg gctgtggcaa ccgaatcatt tgcagatcat gcaggaacat
     8401 ctgcgcgact ttgccgtaga acagagcgat ggtgaagtgt tgatcatcag ccgcacagtt
     8461 attgccccgc cggtgtttga cttcgggatg cgctgcacct acatctggcg catcgctgcc
     8521 gatggccagg ttaacgtggc gctttccggc gagcgttacg gcgactatcc gcacatcatt
     8581 ccgtgcatcg gtttcaccat ggggattaac ggcgaatacg atcaggtggc gtattacggt
     8641 cgtggaccgg gcgaaaacta cgccgacagc cagcaggcta acatcatcga tatctggcgc
     8701 agcaccgtcg atgccatgtt cgagaactat cccttcccgc agaacaacgg caaccgtcag
     8761 catgtccgct ggacggcact gactaaccgc cacggcaacg gtctgctggt ggttccgcag
     8821 cgcccaatta acttcagcgc ctggcactat acccaggaaa acatccacgc tgcccagcac
     8881 tgtaacgagc tgcagcgcag tgatgacatc accctgaacc tcgaccacca gctacttggc
     8941 ctcggctcca actcctgggg cagcgaggtg ctggactcct ggcgcgtctg gttccgtgac
     9001 ttcagctacg gctttacgtt gctgccggtt tctggcggag aagctaccgc gcaaagcctg
     9061 gcgtcgtatg agttcggcgc agggttcttt tccacgaatt tgcacagcga gaataagcaa
     9121 tgaggatcat cgataactta gaacagttcc gccagattta cgcctctggc aagaagtggc
     9181 aacgctgcgt tgaagcgatt gaaaatatcg acaacattca gcctggcgtc gcccactcca
     9241 tcggtgactc attgacttac cgcgtggaga cagactccgc gaccgatgcg ctatttaccg
     9301 ggcatcgacg ctattttgaa gtgcattact acctgcaagg gcagcaaaaa attgaatatg
     9361 caccgaaaga gacattacag gtagtggaat attatcgtga tgaaactgac cgtgaatatt
     9421 taaaaggctg cggagaaacc gttgaggtcc acgaagggca aatcgttatt tgcgatatcc
     9481 atgaagcgta tcggtttatc tgcaataacg cggtcaaaaa agtggttctc aaagtcacca
     9541 tcgaagatgg ttatttccat aacaaataac aactacggcg gcaaaaggag tttgccgcca
     9601 ccgctaccct acacattttc ggagatgtgt tatgtctgat accaaacgta atacaatcgg
     9661 caaattcggc ttgctctcgc tgacttttgc cgccgtttac agctttaaca acgttatcaa
     9721 taataatatt gagcttggac tggcctcggc accgatgttt ttcctcgcga cgatttttta
     9781 ttttattccc ttctgtctga tcatcgcaga atttgtttcg ttaaataaaa actcagaagc
     9841 cggtgtctac gcgtgggtaa aaagttcgct gggcggacgt tgggcattta ttactgccta
     9901 tacctactgg ttcgtaaacc tgttcttttt cacctcactg ttgccgcgcg ttattgctta
     9961 tgcttcgtat gccttcctcg gatacgaata tattatgacg ccggttgcca ccaccattat
    10021 cagtatggtg ctgttcgcct tctccacctg ggtttccacc aacggggcga aaatgttggg
    10081 gccaattacc tccgtcactt caacgctgat gctgctgtta acgctctcct acattttact
    10141 ggcaggtacg gcgctggttg gcggcgtaca gcctgctgac cccatcaccg ttgacgcgat
    10201 gatcccgaac ttcaactggg cgttcctcgg cgttaccacc tggatcttta tggccgcagg
    10261 tggcgcggag tccgtcgctg tgtacgttaa cgacgtcaaa ggcggttcga aatcgttcgt
    10321 taaagtgatc atcctcgccg ggatttttat cggcgtactg tattccgtct cctcggtgct
    10381 gattaacgtc ttcgtcagca gcaaagagtt gaaatttacc gtcggatcgg tgcaggtatt
    10441 ccacggcatg gcggcgtatt ttggtctacc ggaagcgttg atgaatcgct ttgtcggtct
    10501 ggtgtccttt accgcgatgt tcggttccct gctgatgtgg accgcaacgc cggtgaaaat
    10561 tttcttctcc gaaatcccgg aaggcatctt tggtaagaaa accgtcgaac tgaacgaaaa
    10621 cggcgttccg gcgcgcgcag cgtggatcca gttcctgatc gtcatcccgc tgatgattat
    10681 cccgatgctc ggttccaata ccgtgcagga tctgatgaat actattatta atatgaccgc
    10741 cgcagcgtcc atgcttccgc cgttattcat catgctggct tacctgaatt tacgcgccaa
    10801 attagatcac ctgccacgcg atttccgtat gggctcccgc cgcaccggta ttatcgttgt
    10861 ttcaatgctg attgcgatat ttgccgtagg gtttgtcgct tcgacattcc cgactggcgc
    10921 gaatattctg accatcattt tttataacgt cggcggtatt gttatcttcc ttggctttgc
    10981 gtggtggaaa tacagtaaat atataaaggg attaacggct gaagagcgcc atattgaagc
    11041 gacgccagcc agc
//
LOCUS       DAZGOB010000148        11012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGOB010000148 DAZGOB010000000
VERSION     DAZGOB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.148"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            146..907
                     /gene="adiY"
                     /locus_tag="ACHLIF_004276"
     CDS             146..907
                     /gene="adiY"
                     /locus_tag="ACHLIF_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO9608172.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1044..2381
                     /gene="adiC"
                     /locus_tag="ACHLIF_004277"
     CDS             1044..2381
                     /gene="adiC"
                     /locus_tag="ACHLIF_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO9608173.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2485..4128
                     /gene="eptA"
                     /locus_tag="ACHLIF_004278"
     CDS             2485..4128
                     /gene="eptA"
                     /locus_tag="ACHLIF_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO9608174.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4125..4793
                     /gene="basR"
                     /locus_tag="ACHLIF_004279"
     CDS             4125..4793
                     /gene="basR"
                     /locus_tag="ACHLIF_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO9608175.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4794..5894
                     /gene="pmrB"
                     /locus_tag="ACHLIF_004280"
     CDS             4794..5894
                     /gene="pmrB"
                     /locus_tag="ACHLIF_004280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO9608176.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5870..5959)
                     /gene="pmrR"
                     /locus_tag="ACHLIF_004281"
     CDS             complement(5870..5959)
                     /gene="pmrR"
                     /locus_tag="ACHLIF_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO9608177.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6071..7573)
                     /gene="proP"
                     /locus_tag="ACHLIF_004282"
     CDS             complement(6071..7573)
                     /gene="proP"
                     /locus_tag="ACHLIF_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO9608178.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7837..8715)
                     /gene="rdcB"
                     /locus_tag="ACHLIF_004283"
     CDS             complement(7837..8715)
                     /gene="rdcB"
                     /locus_tag="ACHLIF_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO9608179.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8712..10940)
                     /gene="crfC"
                     /locus_tag="ACHLIF_004284"
     CDS             complement(8712..10940)
                     /gene="crfC"
                     /locus_tag="ACHLIF_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO9608180.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALASANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2756 a         2858 c         2709 g         2689 t
ORIGIN      
        1 agaaattact ataaaaattt gtaatattag taaaactcgt tatttttatg catgtttata
       61 ttcatcatac aattatataa ccatttcccg gtatcgcttt gctttagcga gaactggtct
      121 tttatttgca ctcaggagta catgtatgag gatttgcagc gaccaacctt gtattgtttt
      181 attgactgaa aaagatgtct ggataagggt gaatgggaaa gaacctatta gccttaaagc
      241 taaccatatg gcgttattaa attgtgaaaa taatattatc gacgtctcct ctcttaacaa
      301 cactttggtt gctcatatta gtcacgacat catcaaagat tacctccggt ttctgaataa
      361 agatctctcg caaataccag tatggcaacg tagcgctacg cccatactca ccctgccatg
      421 cctgacgcca gacgtctttc gcgttgccgc gcaacacagc atgatgcccg cagaaactga
      481 gtcagaaaag gaacgaacac gcgcattatt attcacggtg ctatcccgtt ttctcgacag
      541 taaaaaattc ctttcattaa tgatgtatat gttacgtaat tgtgtaagtg acagcgttta
      601 tcaaattatt gaaagcgata ttcacaaaga ctggaatctt agtatggtag ccagttgttt
      661 atgtcttagc ccaagtctgt taaagaaaaa gttgaaaagc gaaaacacca gttatagcca
      721 aataatcacc acctgccgca tgcgttatgc cgtaaatgaa ttaatgatgg acggtaaaaa
      781 tatctctcag gtatcacagt cctgcggcta caacagtacg tcgtacttta tttctgtctt
      841 taaagacttc tacggtatga cgccgctgca ttatgttagt cagcacagag aacgcactgt
      901 cgcctgattt ttaaccttaa cgaagagcta tattaataac ggcatcagcg ataacccggt
      961 cgataataat tcaactatcg aatgcaggcg tatgatatga cgtaatttat tgtcacgaag
     1021 ctcgccttcg caggagttta attatgtctt cggatgctga tgctcacaaa gtgggcttaa
     1081 tccccgtcac cctgatggtg tcggggaata ttatggggtc aggtgttttt ctgttacctg
     1141 caaacctggc ctctactggc gggattgcca tttatggatg gttggtgacg attatcggtg
     1201 cgctggggct ctcgatggta tacgccaaaa tgtcgttcct cgatccaagt cctggtggtt
     1261 cttacgcgta cgcccgccgc tgctttggcc cgtttctcgg ttatcaaacc aacgtcctct
     1321 actggctggc ctgctggatc ggcaatatcg ccatggtggt cattggcgta ggatatttaa
     1381 gttacttctt cccgattctg aaagatccat tggtattaac catcacctgc gtcgtggtgc
     1441 tgtggatctt cgtcctgctg aacattgtcg gtccgaaaat gatcacccgt gtgcaggccg
     1501 ttgccaccgt gctggcgctg atccccatcg tcgggattgc cgtatttggc tggttctggt
     1561 tccgtggtga aacctatatg gcggcatgga acgtcagcgg cctgggcacc ttcggtgcaa
     1621 ttcaaagtac ccttaacgtt acgctgtggt cgttcatcgg tgtggaaagt gcctccgttg
     1681 ccgcaggtgt ggtgaaaaac ccgaaacgca atgtccctat cgccaccatt ggtggggtat
     1741 tgattgccgc cgtttgctat gtactttcta ccaccgcgat tatggggatg atccctaatg
     1801 ccgcactgcg cgtttctgct tcgccattcg gtgatgccgc acggatggcg ttgggtgaca
     1861 ccgccggggc cattgtttcc ttctgcgcag ctgcgggttg cttaggttca ctgggcggct
     1921 ggacgttgct ggcgggtcaa acggcgaaag ccgctgccga tgacggactg ttcccaccga
     1981 tttttgcccg tgtaaataaa gcgggcacgc cagtggcggg gctgattatc gtcggtattt
     2041 tgatgaccat cttccagctc agcagcattt caccaaacgc gaccaaagag ttcggtctgg
     2101 tttcttccgt ctcggtcatc tttacactgg tgccatatct ttacacctgt gcggcgttac
     2161 tgctgctcgg tcacggtcac tttggtaaag cacgcccggc atatctggct gttactacca
     2221 ttgccttcct ctactgcatt tgggccgtgg tggggtccgg ggcgaaagag gttatgtggt
     2281 catttgtcac cctgatggta atcaccgcca tgtatgcgct gaattacaac cggctgcata
     2341 aaaacccgta tcccttagat gtaccaataa gcaaagatta attcccctta atccagcaaa
     2401 cataaaagcc aaccttaaga acttaaggtt ggcttaattt tgctttgcga gcatatgcgc
     2461 actttgttcg atggaaacac cgtgatgttg aagcgcctac taaaaagacc ctctttgaat
     2521 ttactcgcct ggctattgtt ggccgctttt tatatctcta tctgcctgaa tattgccttt
     2581 tttaaacagg tgttgcaggc gctgccgctg gattcgctgc ataacgtact ggttttcttg
     2641 tcgatgccgg tcgtcgcttt cagcgtgatt aatattgtcc tgacactaag ctctttctta
     2701 tggcttaatc gaccactggc ctgcctgttt attctggttg gcgcggctgc acaatatttc
     2761 ataatgactt acggcatcgt catcgaccgc tcgatgattg ccaatattat tgataccact
     2821 ccggcagaaa gttatgcgct gatgacaccg caaatgttat taacgctggg attcagcggc
     2881 gtgcttgctg cgctgattgc ctgctggata aaaatcaaac ctgccacctc gcgtctgcgc
     2941 agtgttcttt tccgtggagc caatattctg gtttctgtac tactgatttt gctggtcgcc
     3001 gcactgtttt ataaagacta cgcctcgttg ttccgcaata acaaagagct ggtgaaatcc
     3061 ttaagcccct ctaacagcat tgttgccagc tggtcatggt actcccatca gcgactggca
     3121 aatctaccgc tggtgcgaat tggtgaagac gcccaccgca acccgttaat gcagaacgaa
     3181 aaacgtaaaa atttgaccat cctgattgtc ggcgaaacct cgcgggcgga gaacttctcc
     3241 ctcaacggct acccgcgtga aactaacccg cggctggcga aagataacgt ggtctatttc
     3301 cctaataccg catcttgcgg cacggcaacg gcagtttcag taccgtgcat gttctcggat
     3361 atgccgcgtg agcattacaa agaagagctg gcacagcacc aggaaggcgt gctggatatc
     3421 attcagcgag cgggcatcaa cgtgctgtgg aatgacaacg atggcggctg taaaggtgcc
     3481 tgcgaccgcg tgcctcacca gaacgtcacc gcgctgaatc tgcctggtca gtgcatcaac
     3541 ggcgaatgct atgacgaagt gctgttccac gggctggaag agtacatcaa taacctgcag
     3601 ggtgatggcg tgattgtctt acacaccatc ggcagccacg gtccgaccta ttacaaccgc
     3661 tatccgccgc agttcaggaa atttacccca acctgcgaca ccaatgagat ccagacctgt
     3721 agcaaagagc aactggtgaa cacttacgac aacacgctgg tttacgtcga ctatattgtt
     3781 gataaagcga ttaatctgct gaaagaacat caggataaat ttaccaccag cctggtttat
     3841 ctttctgacc acggtgaatc gttaggtgaa aatggcatct atctgcacgg tctgccttat
     3901 gccatcgccc cggatagcca aaaacaggtg ccgatgctgc tgtggctgtc ggaggattat
     3961 caaaaacggt atcaggttga ccagaactgc ctgcaaaaac aggcgcaaac gcaacactat
     4021 tcacaagaca atttattctc cacgctattg ggattaactg gcgttgagac gaagtattac
     4081 caggctgcgg atgatattct gcaaacttgc aggagagtga gtgaatgaaa attctgattg
     4141 ttgaagacga tacgctgtta ttgcagggat tgattctggc ggcgcaaact gaaggctacg
     4201 cgtgcgatgg cgtgacaacc gcgcggatgg cggaacaaag ccttgaggcc ggtcattaca
     4261 gcctggtggt actggattta gggttacccg acgaagatgg actgcatttt ctcgcccgta
     4321 tccggcagaa aaaatacacc ctgccagtac tgatcctcac cgctcgcgat acgctgaccg
     4381 acaaaatcgc tgggctggat gtcggtgccg atgactatct ggtgaagcct tttgcgctgg
     4441 aagagttaca cgcccgtatc cgcgccctgc tacgacgcca taataatcag ggcgaaagtg
     4501 agctgattgt tggcaatctg acgctgaata tgggtcgccg tcaggtatgg atgggcggtg
     4561 aagagttgat tctgacgccc aaagaatatg ctctgctgtc acggttaatg ctcaaagcag
     4621 gcagtccggt gcatcgggaa attctctaca acgacatcta taactgggac aatgaaccct
     4681 cgaccaacac cctggaagtg catatccaca atctgcgcga caaagtgggc aaagcccgta
     4741 tccgcaccgt gcgcggcttt ggctatatgc tggtcgcgaa tgaggaaaac taattgaatc
     4801 tgatgcattt tctgcgccga ccaatatcgc tgcgccaacg gctgatattg accatcgggg
     4861 ccattttgtt ggtgtttgag ctgatcagcg tcttctggct atggcatgaa agtaccgagc
     4921 agattcagct gtttgagcag gcgctacgcg acaatcgcaa caacgatcgc cacatcatgc
     4981 gtgagatccg cgaggcggtc gccagcctga ttgtccccgg cgtctttatg gtcagcctga
     5041 cgctatttat ctgctatcag gcggtgcgcc gcatcacccg cccgctggcg gagctgcaaa
     5101 aagagctgga agcgcgcact gccgacaact taacgcccat tgccattcac agcgccaccc
     5161 tcgaaatcga agcggtggtt tcggcgttaa acgatctggt cagtcgcctg accagcacgc
     5221 tggataacga aaggttgttt accgctgacg tcgcgcacga actgcgaacg ccactggcgg
     5281 gggtgcgttt gcatctggaa ctgctggcga aaacgcatca cattgatgta gcaccgttag
     5341 tggcacgact tgatcagatg atggagagcg tctcccagct gctgcaactg gcgcgtgccg
     5401 gacagtcatt ttcttccggt aattatcaac atgtaaaact gctggaagat gtcattctcc
     5461 cctcgtatga cgaactcagt accatgctcg accagcgaca gcaaaccctg ctattgccag
     5521 agagcgccgc cgacatcacc gttcagggtg atgcaaccct gctgcggatg ttattgcgaa
     5581 acctggtaga aaacgcccat cgttacagcc cgcaaggcag caacattatg attaagctgc
     5641 aagaagatgg cggagcggtc atggcagttg aagatgaagg accaggtatt gatgagagta
     5701 aatgcgggga gttgagtaaa gcgtttgtac gtatggacag ccgttatggc gggattggtc
     5761 tggggttaag tattgtcagc cgcattacac agttgcatca cgggcagttt ttcctgcaaa
     5821 accggcaaga gacttccggc acgcgggcct gggtacggct gaagaaagat cagaacgtgg
     5881 caaaccagat ataaagaaag ctgctgacca ccagcacgct gaacacggta gttaaacttt
     5941 cataaacacg gtttttcatt gcactctcct ccgttaacct ggaggagagt atgcgcgtca
     6001 gagattaagt gaaccttaag agttcaattg gcctgataag acagcgtcac atcaggccat
     6061 ccgtttcagc ttattcatca attcgcggat gttgctgcac caggcgggta cgtttcgcct
     6121 gcaaatcggc aatctcgtgg tcaatattat cgattttctg ctcgatatta tcgtaatgct
     6181 cgacgagaat ttccttcgct tcctgtatat ctgacgccgc cggtgtcgca cctttcaacg
     6241 gacgatttgc cgtctctttc atggttacgc cggtgattaa accaatcacc gccactacca
     6301 tcaggtaata ggcaggcatc atcagattct gcgagctttc gaccagccag gccgccagtg
     6361 ttggcgtcag accggcaacc agcaccgaaa tattaaatgc cgccgccagc gcgctgtaac
     6421 ggatatgcgt cgggaacatc gctggcaagg tagaagccat aacgcccgta aagcagttaa
     6481 ggatcaccgc cagcatcagt aacccggcaa aaatcaggcc gatgacgtta ctgttaatca
     6541 gaataaacgc cgggatcgcc aacacaaaca gggcaacact accaagtagc acaaacggac
     6601 gacggccaaa acggtcactc agcaagccca tcaccggctg gacaaacagc ataccgatca
     6661 taatggcgat aataatcagc accccgtggt cttcggagta atgcaggtta tgcgacaaat
     6721 aactcggcat ataggtcagc aacatgtagt aagtcacgtt ggtggcaatt accagaccaa
     6781 tacatgtcaa caggctgcgc cagtgtttgg tggcaatctc tttaaacgag actttcgggc
     6841 catcctgcaa accttcgcgg tcgccctgtt ccagtttatc gacatgctgc tggaacgccg
     6901 gagtttcttc cagcgcatgg cgcaggtaaa gcccgataat ccctaacggc agagcaataa
     6961 agaacggaat acgccagccc cagtcgagga agttcgcttc gccgacaatg gtcgaaatta
     7021 acaccactac gccagcaccc agcacaaacc cggcaataga accgaagtcc agccagctgc
     7081 ccataaagcc acgtttacgg tcaggtgagt attccgcaac aaatatcgac gccccggtat
     7141 attcaccgcc gaccgagaaa ccttgtgcca tcttacagat caacagcaga atcggtgccc
     7201 aaataccaat cgtgtcgtag gacggtatta agccaataca gaacgtactg atcgacataa
     7261 tcacaatagt gatagcgagg atcttctggc gaccatattt atcgcccaac ataccaaaga
     7321 agagcccgcc aagcggtcga atcagaaagg gaacggagaa agtggcaagt gcagcaacca
     7381 tctgcacgct ggggtcagcc cccgggaaaa aaactttacc taatgcgtaa gcaacaaaac
     7441 cataaacacc aaaatcaaac cattccattg cattacccag tgatgctgcg gtaatggctt
     7501 tacgcagttt accgtcatca ataatggtga catcacgaag ggtaattggt tttacttttt
     7561 tccttttcag catagctttc ctcgcagagt tggcccattt ccgcaaacgc cagaaaaatc
     7621 agcgcgacgg ccgcaaaagg ggagcgggaa ttgcaggatg caatctctgt aacaagcgta
     7681 gcgggtttgc ttacaccctc cggttaagga atgtacaatc aaaccagttg acgcctgaat
     7741 tgggcagtta atgacctttt taccctacca gtgtttaaat ttgtgatcaa cttcacacat
     7801 ggatttcgtt taggactcat tgatgtaact gattatttac gccaaattta cagagaagat
     7861 ttcctcgagc attgcccccg ccagacgctg cgctgaacag agtcgtggca tccccagcgc
     7921 gacagtttgc cagcgttggg tcactgacca gcaaacgggt tgtttatctg ccgtggtcca
     7981 ctttcccagc caacttaaaa aatcagcatg atccccgccc gtcggaacgg tcgaagaggc
     8041 aaaccactga tggtaatagt gacgcgtggt cggggccgca ttgatactct ctgccagatg
     8101 ttgtgtcgct ttcacgcgca aatcacccag caacaacgcc accgcttgcg ggcttcccag
     8161 acgacaggca tcgccaaatg ccccccaacg caactcttcg agaacgataa aacagcgccc
     8221 aggcaacgac caggcgttat acgccccagc acgccagcgg gtaaaaattt gctcgctctg
     8281 atcacctgcc tgcaccagta ggcctcgttg cgttaacgcc ttttccttcc agcggcaacg
     8341 ggctgaaaac cactcataca aggcggcaat ttgctggggg agctccggga aagcagagtg
     8401 ctggcggtga tccatttttt gcagcagcgc cagatgctgc gcgatctgcg tcagtgccag
     8461 atgaccggga tccagcatcg acgccagttt ttccaccaat gtcagacgca ggctaagcgg
     8521 cgcatttaac atgccgccgt tcgtccatgt ctgaagctgc acgcgcgcaa gaagttcctg
     8581 catcagtcgt tcacgaaact gctgctgatg ggcttgtgga aggcgggcat catcgccctg
     8641 cgccagatcc actaaaaagc ggggataaac cgactccagc acgcgaccgg ggccgtcaag
     8701 taacgtcttg gtcaatatcg ttctgccgtg aaaagtgttt gaatatcatc gcgtaacagc
     8761 tgggcgtcgg tgtaaatcca gccgtgagtc atcacagtcc gctgcaattg ctgctgcatc
     8821 agcctgacca ccgattcatt ttgttgacgc agagccagac tttcgcgtaa acgcgtctgt
     8881 aattccgtca aacatgaagc gaactcagcg aaaaaagtat tcatgcctgc cgtaacagat
     8941 tcatcgacct gctctgccag aactttagcc atttgttggc aataaagatc gacttctgcg
     9001 cttaaagctc gttgcaacac actgtaatca accgtctctg tcggggattt ctcatttccc
     9061 cgtccccagt cgggctgatt caaccagcgc gaaaaagtct cacgcacaac gcctaaacgc
     9121 gtgctctgct cgtccgttgc ttcctggcgc gaaatgactt cactgaacag ctggcgagtg
     9181 ttgaagtggg gaactgcgcc gtgaaaaaca ggaaaatgaa acccaggacg aaaccctgac
     9241 tcgctcaatt ccattttgac ttgttgctca atggggcgaa taacatcggt taacacccgg
     9301 caaagggtgg attccagctc ggcaaaacgc agcgtaaagt cgcgactgat ggtgttctgc
     9361 gccgtctgta acagtgtctc acagcgggta cgaatctcgc ttaacggctc cgaatcatcc
     9421 tgaaacaacg cggctaactg tgcattcacc gcatcttgct gttgacgcag aaagtggttg
     9481 gcggaggcca aggccagctc gatttcatgt ttaatctcgc cgctcacctg cgcctgattg
     9541 agttgcaata gctgcaaact ttcttcgacc tgatggatat tttgccgcaa ttgttcacaa
     9601 gcgacgttta acccgtgcgc acgaaaatcc aggtattccc gcgcctgctg cgcgtaattc
     9661 aacagtttat gcgcggcaga tcgcagagca tacaacgagg cgttagcgta agcggcatga
     9721 agcaacgcct gaattggctg ggcgaacagc gaatcttccc acaactgatc ggcagcatga
     9781 cgaatatgtt cgaggtccgc cagatcggca tgacgccagc gcctgccgag cgcggcatgg
     9841 gcaaaatctt ccacccagcg ttgttgctct ggcggtggta acttaccgtt gttggctaac
     9901 tcatggcgcg cccgattcgc caggtagccc cacatcgacg acaccggaaa tatctgctgt
     9961 ggcgtaatac agcctttcat cagcgtcccg gaaatcagtg cccgcacctg gtcggcgtcg
    10021 tcactgttac gatcctgttg atcgaactta ttgaccagca catacagcgg caccgattgc
    10081 cccaccgcca aaatcgcctc acggacctct tcatcggaga tcgatttcag ttgcgtataa
    10141 tccagcaccg ccagtaccgc cgaggcgcgt gccagctgct ggttaagcat tttttgcaga
    10201 tgcggttgcc cggcttcatt tggcccgggg gtatccagta acgtcaactg accgggataa
    10261 ctttccagcc ccgccagatg gacaaactcc acttcaatca cgggaatatg ctcaatggcg
    10321 gcgtaagcag aaaaaggaaa atcgacgtcc agcgccttcg ccagtcgcac taaatcattc
    10381 aaacttttca gacaatgaaa aataggctgg gcacccagat aatatttttc gaaagcgacg
    10441 ccattttcga tccgctgcat aagcgcacgc atatctttat ctatttccag cacatcggtc
    10501 agatgcttaa tatcgcaatc acgcaggcgc tgttgtaatt tttgaattaa acggtcgatt
    10561 ggcgcgacat gtgaaaaatg cagtaccggt tctttttgcc cgggcgtatg gcgaataagc
    10621 gtcggcagcg cagtcattgg gcgattacga ttaggcaaaa cctccgtacc aacaatggca
    10681 ttaatggtgg ttgatttccc tgctttcatg gtaccgacaa tcgccagcac catttccagt
    10741 cgggaaattt tacgcaactc attattcagc atcgcgtgac gttcggcgat attaggctga
    10801 ctccagggta aagccagttg tggcgcatcg tctccgggta cagagagagg cattttttcc
    10861 agtaactgca actgttggcg agaaagctgt aacaggcgtt cagcctcctg acttaactca
    10921 tacagggtct gtgtgtacat agaaaattct tccttaaagc aaattttgtt acttttattt
    10981 agccagattg tttttgagtt ctgttttcgg ct
//
LOCUS       DAZGOB010000149        10957 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGOB010000149 DAZGOB010000000
VERSION     DAZGOB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.149"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            237..1919
                     /gene="ydeN"
                     /locus_tag="ACHLIF_004285"
     CDS             237..1919
                     /gene="ydeN"
                     /locus_tag="ACHLIF_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9608181.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1971..3128
                     /gene="ydeM"
                     /locus_tag="ACHLIF_004286"
     CDS             1971..3128
                     /gene="ydeM"
                     /locus_tag="ACHLIF_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO9608182.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3094..3216)
                     /locus_tag="ACHLIF_004287"
     CDS             complement(3094..3216)
                     /locus_tag="ACHLIF_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608183.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
     gene            3419..5104
                     /gene="yddA"
                     /locus_tag="ACHLIF_004288"
     CDS             3419..5104
                     /gene="yddA"
                     /locus_tag="ACHLIF_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO9608184.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQSGVWNLADDICDISAVL"
     gene            5142..7514
                     /gene="yddB"
                     /locus_tag="ACHLIF_004289"
     CDS             5142..7514
                     /gene="yddB"
                     /locus_tag="ACHLIF_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO9608185.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSVVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7571..10354
                     /gene="pqqL"
                     /locus_tag="ACHLIF_004290"
     CDS             7571..10354
                     /gene="pqqL"
                     /locus_tag="ACHLIF_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO9608186.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVHDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10716..>10957
                     /locus_tag="ACHLIF_004291"
     CDS             10716..>10957
                     /locus_tag="ACHLIF_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO9608187.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         3242 a         2389 c         2368 g         2958 t
ORIGIN      
        1 tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata aattagttgt
       61 ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta tggactgtta
      121 tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac ttacatccac
      181 ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga acatttatga
      241 agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg gcatctggta
      301 tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg
      361 ttgctttctc agactttacg ccgactgaat acagtaccaa aggaaagcca aatattatcg
      421 tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga tcttttgacc
      481 caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata gataaagcca
      541 ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa ggcgtacggt
      601 ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca ataatgaccg
      661 gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat ggtattccgc
      721 taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact gcagcagtag
      781 gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa caaacgcgtg
      841 actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa aaccgtggct
      901 ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc ccttcactgt
      961 tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta accgatgagg
     1021 caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt tacctggctt
     1081 ataatgctcc gcacctgcca aatgataatc ctgcaccgga tcaatatcag aagcaattta
     1141 ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt gatcagggtg
     1201 taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca attattctct
     1261 ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag
     1321 gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg tggtggaaag
     1381 gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc tacccgacag
     1441 ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggc gtttccttgc
     1501 tgccctggtt gcaagataag aaacaaggcg agccacataa aaatctgacc tggataacct
     1561 cttattctca ctggtttgac gaggaaaata ttccattctg ggataattac cacaaatttg
     1621 ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta agccaattct
     1681 cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac aatcagttag
     1741 gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc aatccgcagg
     1801 tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag ccaccgctta
     1861 gcgaagtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa gcgaaataac
     1921 taaaccttca tgcggcggaa ttttccgccg ccttattgag cgagatagcg atgcacgtta
     1981 cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt taccttgaaa
     2041 aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa gagttcatca
     2101 aacaatatat cgcagcgtct ggcaatcagg tctattttac ctggcaaggc ggtgaaccca
     2161 ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc
     2221 aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat gaatggtgtg
     2281 cttttctcaa agaacatgag tttctggttg gtatctcgat cgatggcccc caggagctac
     2341 atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg atagcagcca
     2401 tcgagcgtct gaaatcatat caagttgagt ttaatacgtt aaccgtcatt aataacgtta
     2461 atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt aaacatatgc
     2521 aatttatcga attgctcgaa accgggacgc cgaatattga tttcagtggt catagtgaga
     2581 acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa
     2641 ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt cagtttgaaa
     2701 gctttgtcag ccgttttttg gggaatgggc ataccagttg tattttccag gagtcctgca
     2761 aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat tttgtctatc
     2821 cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac agtgtacaac
     2881 tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat aaacctatct
     2941 gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact gtttcttatt
     3001 tttgcgaagg ttataaaaac cttttttcaa ccatggtacc ttatatgaac gccatggtgg
     3061 agttagctaa gaacagagta ccgctttatc acattatgga tgttgcaaaa caaatggaga
     3121 ataattaata acacgccctt ttagctccat tcttagcctc cttcttatga ccttaacaag
     3181 gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt gcgaaaatcc
     3241 tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat agcataacca
     3301 taactaatga aaatgaaata tttctttata tttcatattg ttaagtgata cttaaataaa
     3361 tttcatcagt aaatcattgc catagataat cattggatgg cacaatcaat tttcgctaat
     3421 gataaccatt cccattacgt tttgtatgtt gatagcaaag tatctctgtc tgctgaagcc
     3481 cttttggctg cgtaagaata ataaaacttc agtgttgtta atcattatta tcctcgccat
     3541 gatccttggc gtggttaaaa ttcaggtctg gttaaacgac tggaataatg attttttcaa
     3601 tgcgctaagc cagaaagaaa ccgataagct ttggcagctt gtcctttggt tccctgccct
     3661 gctggggatc tttgtattaa tatccgtaaa caaaacatgg ttaattaaac tactgactat
     3721 tcgctggcgc gaatggttaa cagattacta ccttaatcgg tggtttgcag ataaaaacta
     3781 ctacttcacg cagatctacg gcgagcataa aaatacagat aaccctgacc aacgtatcgc
     3841 tgaagacatt ctcttactga tcagcaaaac gctcagcctt tcatttggct tcatccagtc
     3901 acttagcatg ctgatcacct ttaccgttat tctttggcaa agcgcgggta cgctctcatt
     3961 cactgttggt ggaacagaat ggaatatcca ggggtatatg gtctataccg ttgtgctcat
     4021 tgtgatcggt ggaactttat ttacccataa ggttggtaaa cgtattcgtc cgcttaatgt
     4081 ggaaaaacaa cgtagcgaag cgacatttcg aacaaatctt gtgcagcata ataagcaggc
     4141 agaactgatt gcgctaagca atgctgagtc tctacaacgc caggagttga gcgacaattt
     4201 tcataccatt aaagagaact ggcatcgttt aatgaatcgt cagcggtggc ttgattactg
     4261 gcagaatatt tattcgcgct cgcttagcgt tcttccctac tttctgttat tgccgcagtt
     4321 tattagcggg cagatcaatc tgggcggact gatgaaatcg cgccaggcat ttatgctggt
     4381 atcgaacaat ttaagctggt ttatttataa atatgacgaa cttgctgaac tggctgcggt
     4441 tatcgatcgt ttgtatgagt tccatcaact caccgaacag cgccctacga ataaacctaa
     4501 aaattgccaa catgcggtac aagtggctga cgcgagtatt cgtactcctg ataataagat
     4561 catattagag aacctgaact ttcatgtttc gccaggcaaa tggctattac tgaaaggtta
     4621 ctctggtgcg ggaaaaacca cactgcttaa aacattatct cactgctggc cgtggtttaa
     4681 aggtgatatt tcttctcctg ctgacagttg gtatgtgtca caaacaccgt taatcaaaac
     4741 cggcttactg aaagagatta tttgtaaagc acttcccctg cccgtagacg ataaatcgtt
     4801 gagcgaagta ctgcatcagg ttggtctggg gaaattggct gcgcgtattc atgaccacga
     4861 tcgctgggga gatattcttt ccagcggcga aaaacaacgt atcgccctgg cacgattaat
     4921 tttacgacgt ccgaaatgga tatttcttga cgaaactacc tctcatcttg aggaacaaga
     4981 ggctatccgc ttactgcgtt tagtgcgtga aaaactaccc acaagcggcg tcattatggt
     5041 tacacatcaa tccggtgtct ggaacctggc agatgatatt tgtgacatta gcgcggtttt
     5101 ataaaaacaa taaaagccct gctgtacgga gatataaata aatgaagcga gttcttattc
     5161 ctggcgtcat tttatgtggc gctgatgtgg cgcaggccgt cgatgacaaa aacatgtaca
     5221 tgcatgtttt tgaagagatg acggtctatg ctcctgtccc tgtacccgta aacggcaaca
     5281 cgcattacac cagtgaaagc atcgagcgtt taccgaccgg gaatggcaat atcagcgatc
     5341 tgctgagaac caaccctgcg gtacgcatgg attcaacgca aagtacctcg ttgaaccagg
     5401 gagatattcg cccggagaaa atctctattc acggtgcgtc gccctatcag aatgcctatt
     5461 tgattgacgg tattagtgcc actaataact tgaacccagc gaatgagtcc gatgccagta
     5521 gtgcaaccaa tattagcggg atgtcacagg ggtattatct tgatgtcagc ttactggaca
     5581 atgtgacgct ttatgacagt gttgtgccgg ttgaatttgg tcgcttcaat ggcggggtaa
     5641 ttgatgcaaa gatcaaacgc ttcaacgctg atgatagcaa ggtgaaattg ggttatcgca
     5701 ctacgcgttc ggactggtta acatcgcata tcgatgagaa taacaagagc gcatttaatc
     5761 aaggctcttc aggaagtact tattactccc cagattttaa aaagaacttt tataccttgt
     5821 cgtttaatca ggaactcgct gataacttcg gcgttaccgc cggtttatcg cgccgccagt
     5881 ctgatatcac ccgcgcggat tatgtttcga atgacggcat tgtcgccggt cgggcacagt
     5941 ataaaaacgt tatcgatact gcattgagca aatttacctg gtttgccagc gaccgcttta
     6001 cccacgattt aaccttaaaa tataccggct ccagccgtga ttataatacc agcaccttcc
     6061 cgcagtctga tcgcgaaatg ggtaataaat cctacggtct ggcatgggat atggatacgc
     6121 agctcgcatg ggccaaactg cgtaccaccg ttggttggga tcatattagt gattataccc
     6181 gtcacgatca tgacatctgg tacaccgaac tttcatgtac atatggtgat attacagggc
     6241 gttgtacccg tggcggatta ggacacattt cccaggctgt agataattac accttcaaaa
     6301 cacgcctgga ctggcaaaaa ttcgccgtgg gtgatgtttc gcatcaaccc tacttcggcg
     6361 cggaatacat ctattccgat gcatggactg aacgccataa ccagtctgaa tcctatgtga
     6421 ttaatgctgc aggaaagaaa actaaccata ccatttacca taaaggtaaa ggcagcctgg
     6481 gaattgacaa ctacacgctg tatatggcgg atcacattag ctggcggaat gtgtcgttaa
     6541 tgcccggtgt gcgttatgac tatgacaact atctgtcaaa ccacaatatc tccccgcgct
     6601 ttatgacgga atgggatatt tttgctgatc aaacctcaat gattacagca ggttataacc
     6661 gttactatgg cgggaatatt cttgatatgg gattacgtga tatccgcaat agctggacgg
     6721 aatcggtatc aggcaataaa accctgacgc gttatcagga tttgaaaacg ccttataacg
     6781 atgaactggc aatgggattg cagcagaaaa tcggtaagaa cgttattgcg cgcgcaaact
     6841 atgtttaccg tgaagcgcat gatcaaatca gcaaaagcag tcgtaccgac agcgcgacta
     6901 aaaccaccat tactgaatat aacaacgacg gcaaaacaaa aacgcattcg ttcaacctca
     6961 gttttgaact ggccgaaccc ctgcatatca gccaggtaga tattaacccg caaattgtct
     7021 ttagctatat caagagcaag ggcaacttgt cgttaaacaa tggttatgag gagagcaata
     7081 ccggtgataa ccaggtggtt tataacggta atctggtttc ttacgatagc gttccagtgg
     7141 cagattttaa taacccatta aagatctcct taaacatgga tttcacgcat caaccgagcg
     7201 ggttagtgtg ggcgaatacg ctggcctggc aagaagcgcg taaagctcgc attatcctgg
     7261 gtaagacaaa tgcgcaatac atcagcgaat attcagatta caagcagtat gttgacgaaa
     7321 aactggatag cagcctgacc tgggacaccc gcttgtcctg gacgccacaa tttctgaaac
     7381 aacaaaacct gacgatcagt gccgatattc tcaatgtatt ggatagcaaa accgctgttg
     7441 atacaacgaa taccggtgtg gcgacctacg ccagtggccg tactttctgg cttgatgtca
     7501 gcatgaaatt ttaatagttc ggattttact taaccgggta aattcgcccg gttttcgcat
     7561 ggagataatt atgagaaacc tctgtttctt actgacgtta gtggcaactc tgttgctccc
     7621 cgggcggctg attgccgccg ccttaccgca ggatgaaaag ttaattaccg ggcaactggg
     7681 caatggcttg cgatatatga tttatccgca tgctcatcca aaagatcagg taaatttatg
     7741 gctgcaaatt cataccggtt cattgcagga agaagacaat gagcgcggcg tggctcattt
     7801 tgtagaacat atgatgttta acggcacaaa aacatggccg ggtaataaag tcatcgaaac
     7861 atttgagtca atgggcctgc gttttggtcg cgatgttaat gcctatacca gctatgacga
     7921 aacggtgtat caggtgagtt tgccgactac gcagaaacaa aatctgcaac aagtgatggc
     7981 aatcttcagt gaatggagta atgccgcaac ctttgaaaaa ctcgaagtag acgctgaacg
     8041 tggcgtaatt actgaggaat ggcgtgccca tcaggatgcg aaatggcgca cctctcaggc
     8101 gcgccgccct ttcctgctgg ctaatacccg taatttagac cgtgaaccta tcggcctgat
     8161 ggatactgtc gccacggtca caccggcaca attgcgccaa ttttatcaac gctggtatca
     8221 accaaataat atgaccttta tcgtggttgg cgatatcgac agtaaagaag cgctggcgct
     8281 gataaaggat aatttaagta agcttccggc taacaaagca gctgaaaatc gcgtctggcc
     8341 gacaaaagcc gaaaaccacc tgcgctttaa tatcattaat gataaagaaa accgggtgaa
     8401 cggcatcgca ctctattatc gcctgccaat ggtacaagtg cacgatgagc aaagctttat
     8461 cgaacaagct gaatggagca tgttagttca gctgttcaat caacgtctgc aggaacgcat
     8521 acagtcgggc gagttgaaga ctatttctgg cggcactgcg cgcagcgtta aaattgcacc
     8581 cgattatcag tcgctgtttt tccgtgtaaa tgcacgagac gataatatgc aggatgctgc
     8641 gaatgcatta atggcagagt tggcaactat tgatcagcat ggtttttctg ctgaagaact
     8701 cgatgatgtt aaatctaccc gcctcacctg gctgaaaaat gcggttgatc agcaagctga
     8761 acgtgattta cgtatgctga ccagtcgcct ggcatccagc tcattaaata atacgccgtt
     8821 cttgtcgccg gaagagacat atcaactttc gaaacgtctg tggcagcaaa ttaccgtgca
     8881 aagtctggcg gaaaaatggc agcagttaag aaagaaccag gacgcatttt gggagcaaat
     8941 ggtaaacaat gagcttgccg ccaaaaaagc attgtctcct gcggctatcc tggcgctgga
     9001 aaaagagtac gccaacaaaa agctggcggc ttacatcttc ccaggcagaa atttatcgtt
     9061 aacagtagac gctgacccac aggcggaaat tagcagcaaa gaaacgctgg ctgagaatct
     9121 gacatcatta acactttcca atggtgccag ggttattctg gcaaaatccg cgggtgaaga
     9181 gcaaaagcta caaattactg ccgtatcgaa taaaggcgat ttaagtttcc ctgcgcagca
     9241 aaaatcactt atcgcgctgg caaataaagc agtaagcgga agcggcgttg gtgagctctc
     9301 ctcttccagc ctgaaacgct ggagtgcgga aaattcggta accatgagca gtaaagtcag
     9361 tggcatgaat acgttgctct ccgttagcgc gcggactaat aaccctgaac ctggttttca
     9421 gttgattaac cagcgaatca cccacagcac gattaacgac aatatttggg catcgctaca
     9481 aaatgctcaa attcaggcgt tgaaaacgct cgaccagcgt ccagcggaga aattcgccca
     9541 gcagatgtat gagacgcgct atgctgatga ccgcacgaaa ttactgcaag aaaatcagat
     9601 tgtacagttt actgccgcag atgcgctggc tgccgatcgc cagttgtttt catctccagc
     9661 ggatatcacg tttgtcattg tcggtaatgt ctcagaagac aaactcgtgg cgttaattac
     9721 gcgttactta ggatcaatca aacactctga ttcgccatta gccgcaggta aaccattaac
     9781 tcgcgcgacg gacaacgcat cggttactgt aaaagaacaa aatgaacctg tggcacaggt
     9841 ttcacagtgg aagcgttatg attcccggac acctgttaat ctggcgacgc gtatggcgct
     9901 cgatgctttt aacgtcgcac tggcaaaaga tctacgtgtt aatattcgtg aacaggcatc
     9961 tggagcatac agcgtttctt ctcgcctctc ggttgatcct caggccaaag atatcagtca
    10021 tttgctggct tttacttgtc aaccagaacg acatgatgaa ctgttaacgt tagcgaatga
    10081 agtgatggtt aagcgtctgg ctaaagggat cagtgagcaa gaactgaatg aataccagca
    10141 aaacgttcag cgcagcctcg atatccaaca gcgtagcgtt caacaattag cgaacactat
    10201 tgtaaatagt cttattcaat atgacgatcc tgcagcatgg actgagcagg agcaattgtt
    10261 gaaacaaatg acggtagaga atgttaacac tgccgttaaa caatatcttt ctcatccggt
    10321 aaatacttat accggagtat tattgccaaa ataataacag ccccgtcaac acatcgttgg
    10381 cggggatttt agcaatattc gctattttta tgtaataatt ttataaatgc gttcaaaata
    10441 ataatcaagt actaatagtg atattttaag gtctgatttt tacgtgataa ttcaggagac
    10501 acagaatgcg cataaaaata acagcataaa acaccttacc accacccaag aatttcatat
    10561 tgcattgttt ttcaatgaaa aaatattatt cgcgtaatat ctcacgataa ataacattag
    10621 gattttgtta tttaaacacg agtcctttgc acttgcttac tttatcgata aatcctactt
    10681 ttttaatgcg atccaatcat tttaaggagt ttaaaatgga taagaagcaa gtaacggatt
    10741 taaggtcgga actactcgat tcacgttttg gtgcgaagtc tatttccact atcgcagaat
    10801 caaaacgttt tccgctgcac gaaatgcgcg acgatgtcgc attccagatt atcaatgacg
    10861 aattatatct tgatggcaac gctcgtcaga acctggccac tttctgccag acctgggacg
    10921 acgaaaatgt ccacaaattg atggatttat ccattaa
//
LOCUS       DAZGOB010000150        10690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGOB010000150 DAZGOB010000000
VERSION     DAZGOB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.150"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            74..787
                     /gene="yghS"
                     /locus_tag="ACHLIF_004292"
     CDS             74..787
                     /gene="yghS"
                     /locus_tag="ACHLIF_004292"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9608188.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            819..1577
                     /locus_tag="ACHLIF_004293"
     CDS             819..1577
                     /locus_tag="ACHLIF_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608189.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1623..2972
                     /locus_tag="ACHLIF_004294"
     CDS             1623..2972
                     /locus_tag="ACHLIF_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO9608190.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            2972..3796
                     /locus_tag="ACHLIF_004295"
     CDS             2972..3796
                     /locus_tag="ACHLIF_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608191.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            3808..4368
                     /locus_tag="ACHLIF_004296"
     CDS             3808..4368
                     /locus_tag="ACHLIF_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO9608192.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4399..5469
                     /gene="lptF"
                     /locus_tag="ACHLIF_004297"
     CDS             4399..5469
                     /gene="lptF"
                     /locus_tag="ACHLIF_004297"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO9608193.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5466..6545
                     /gene="lptG"
                     /locus_tag="ACHLIF_004298"
     CDS             5466..6545
                     /gene="lptG"
                     /locus_tag="ACHLIF_004298"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO9608194.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     VLVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6581..7753)
                     /locus_tag="ACHLIF_004299"
     CDS             complement(6581..7753)
                     /locus_tag="ACHLIF_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO9608195.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYSSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQINAIIQAFATLKQ"
     gene            complement(7753..8001)
                     /locus_tag="ACHLIF_004300"
     CDS             complement(7753..8001)
                     /locus_tag="ACHLIF_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO9608196.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8033..8947)
                     /locus_tag="ACHLIF_004301"
     CDS             complement(8033..8947)
                     /locus_tag="ACHLIF_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO9608197.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDSVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8944..10632)
                     /locus_tag="ACHLIF_004302"
     CDS             complement(8944..10632)
                     /locus_tag="ACHLIF_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO9608198.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         2561 a         2668 c         2805 g         2656 t
ORIGIN      
        1 acaataaggt tgaacggata ttctgagcac ggaccacgta atattcaata cattatttac
       61 tgccgggttt ttcgtgaatc aacgtaatat gtcaataatt aattccacgc cagtgcgtgt
      121 tattgccatt gtaggatgtg atggttcagg taaatcgacc ctcacggcaa gcctggtaaa
      181 tgaactggca gcaagaatgc caacagaacg catttatctc gggcaatcgt ccgggcgaat
      241 tggcgaatgg atttcacagc tccctgttat tggcgcacct tttgggcgtt atctgcgaag
      301 taaagcggca catgtgcacg aaaagccctc aacaccgcct ggcaatatta ctgcgctggt
      361 tatctatctg ctttcctgct ggcgggcgta caagtttcgc aaaatgttgt gtaaaagcca
      421 gcaaggcttt ctgctcatca ccgaccgcta cccgcaggtt gaagtgccgg gttttcgctt
      481 tgatggcccg caattggcaa aaaccacggg cggtaacggt tggataaaaa tgttaaggca
      541 gcgcgagctg aagctgtacc aatggatggc atcttatttg cccgtattgt tgattcgtct
      601 tggcattgat gaacaaaccg cgtttgcgcg taaacctgac caccaactgg cagcgttaca
      661 ggaaaaaatc gccgttacgc cgcaactgac attcaatggc gcaaagatcc ttgaactgga
      721 tgggcgacac cccgccgatg aaattctgca agcgtcacta cgcgcaattc acgccgcgct
      781 ttcctgatca ccaaaatgga taggggatga tttaacgaat ggatgcacta caaactcaaa
      841 ctgttaatag tacaaccgcg ccgcagccca actacattcc ggggctgatt gcggtggtcg
      901 ggtgtgatgg caccggtaaa tccacactga ccaccgacct ggtgaaatcg ctgcaacaac
      961 actggcaaac cgagcggcgc tatctggggc tgctctccgg cgaagacggc gacaaaatca
     1021 aacgattgcc gttggttggc gtctggctgg aacggcgact ggcggccaaa tcctcgaaaa
     1081 cccaaagcat gaaaaccaaa tctccggcgc tatgggcggc ggtgattatg tactgcttct
     1141 cgctgcgaag aatggcgaat ctacgcaagg ttcagcgact ggcgcaaagt ggcgttctgg
     1201 tggtcagcga tcgcttcccg caggctgaaa tttcgggctt ttattatgat ggaccgggga
     1261 ttggcgtcga acgtgcgacc gggaaaatca gcatgtttct ggcgcagcgc gaacggcgtt
     1321 tataccaaca aatggcgcaa tatcgcccgg aattaattat tcgcctgggc attgatattg
     1381 agactgccat ctcccgcaag cctgaccatg actatgccga gctgcaggac aaaatcggcg
     1441 tcatgtcgac gattggctat aacggcacaa aaattcttga gattgattcc agagcgccct
     1501 acagcgaagt gctggaacag gcacagaagg cggtttctct ggttgccatc gtttctgacc
     1561 gccgaagttt aacttaaggc tggcgggata ctttttacta tcaacgataa ggtcaggctg
     1621 aattggcggg ttttaacatc aaacattggt ttgcagatgg cgcgtttcgc accattattc
     1681 gcaatagcgc ctggttaggc tccagtaatg tcgtgagcgc cttgctgggt ctgttggcgc
     1741 tctcgtgtgc cggtaaaggg atgacgcccg ccatgtttgg cgtactggtg attgtgcaat
     1801 cgtacgccaa gtcgatcagc gattttatta agtttcagac atggcaactg gtggttcagt
     1861 acggaacgcc agccttgacc aacaataatc cgcagcaatt tcgcaatgtc gtctcatttt
     1921 ccttctcgct ggatatcgtc agcggcgcgg tggcgattgt cggcggcatt gccttactgc
     1981 cattcctttc ccattcatta ggtctggatg accaaagctt ttggctggcg gcgctgtatt
     2041 gcacgctcat tccttcaatg gcttcctcca cgccgaccgg cattctgcgt gcggtagatc
     2101 gcttcgattt aattgctgta cagcaggcga cgaaaccttt tctgcgcgca gcggggagcg
     2161 tcgtagcctg gtattttgac tttggttttg cgggttttgt tattgcctgg tacgtgtcga
     2221 atctggttgg cggcaccatg tactggtggt ttgcagcgcg cgaattacgc cgcagaaata
     2281 tccataatgc cttcaaattg aatctgtttg agtctgcccg acacatcaaa ggcgcgtgga
     2341 gttttgtctg gtcaaccaac attgcccact ccatctggtc ggcgcgtaac tcgtgcagca
     2401 cagtgctggt ggggatcgtg ttaggacccg ctgccgccgg gttatttaaa atcgccatga
     2461 cattcttcga cgccgccgga acgccagcgg gtttgctggg taaaagtttt tacccagagg
     2521 tgatgcgttt agatccgcgc accaccagac cgtggttgtt aggtgtgaag tccggtttac
     2581 tggcgggcgg aatcggtatt ctggtggcgc tcgcggtgtt tatcgtcggc aagccgctca
     2641 tttcgctggt gtttggcgtt aagtatctcg aagcgtatga cctgattcag gtgatgctgg
     2701 gcgcaattgt gatttcgatg ctgggcttcc ctcaggagtc attgctattg atggcgggaa
     2761 aacagcgcgc ttttctcgtg gcgcaaacca tcgcctcaat cggctacatc gtattgctgt
     2821 tcatgttctg tcatctgttt ggtgtgctgg gagcagcgtt tgcttacttc ggtgggcaat
     2881 gtctggacgt ggcgctctcg ttaattccaa ccttaaaggc attttttcag cgccattcct
     2941 tgctttataa cgcagccgga gagaaatcct gatgatgaga aaatatttcc cgcttgaggc
     3001 gagcgagcgt ctatttgttg cgattgaaga agatgatgtc gttgatgcac aggttagttt
     3061 gccgccgact attgcactaa gttgtaccac cgaaatcatt catgataatt acgcgttatg
     3121 cctgcaattc tggctcaatg gcgttgatcg tcaggaatta ctgcgtttag ttcgcaaaca
     3181 agccaaaggc gatgagctga ctgctgatga gcgcaaacaa tttaaataca tgcgagcgcg
     3241 ctacaagcac ctgcgttttg cgcaacggct gtatttaaag aagcatcagg ccggattttt
     3301 attcggcaaa acgaccgttt ttctggggcg ttttcaggac ggctttcgta acggtaagaa
     3361 aaatattgtg tcgtattacg gcaacctgtt acggatttat ttaagctctc cggtctggtc
     3421 gttggtgaac tattcattac gccatagcca gttagaaagc gtaagcagtt ttattgccta
     3481 tcggcaaaag caaatgcata ccctgaaaga gattattgcc aaaccgcgac taaccggcag
     3541 ggaatttcat gatgtgcgaa aaatcatcag ccaacaggtt tcttattacg acaccttaag
     3601 atcgcttgat cctgaaaata aagaggcgtt acagatttcc cgtttcctcg ccgccattaa
     3661 tggcctgatg ggagataaac acgacgacat ggtggcggat gatatggaaa accgccagtc
     3721 gtatgatgca ccgttggcgc tggatagcga tattcgtcag cgcttagagc tgctgatttc
     3781 gcgttttccg ctatagccgg agcaatgatg tcatctcgtt tgattattgc gttgattatc
     3841 atgctcttag ccccgggtgt gcaggcacac aatttcgtca ctggcaaaac ggttacccct
     3901 gtttatatcc aggaaggcgg cgagctgctt ctgaatagcg acgacgaaat tcactaccaa
     3961 aagtggaaca gcacacaatt agcaggaaaa gtccgcatta ttcagtatat cgccgggcgt
     4021 aagtcggcga agaaaaagaa ctcactgtta attaaagcgg ttgaagcggc aaactttccg
     4081 caggatcgtt ttcagcccac caccattgtg aacaccgatg atgccatctt tggtactggc
     4141 tattttgttg ttggcaaaat cgaaaaaaat aaacgccgct atccgtgggc gcaattcatt
     4201 attgacggca atggacaggg acgcgttgcc tggcgcttgc cggagcagag ttcaaccatt
     4261 ctggtgctga ataaagccgg gcaaattcag tgggcaaagg acggcagcct gacgccggaa
     4321 gaggtagatc atgtcattgc gctggcgcag aaactgatca atgaataaaa aattcaaagg
     4381 attgagggcg ttagtgagat gaagctggtt gaacactaca tcatgcgtgg tacgcgccgt
     4441 ctggtgctga ttatcgtcgg ttttctgatc tttattttcg ccagctactc cgcacagcgt
     4501 tatctgaccg aagcggcaaa tgggacatta gctcttgatg ttgtgctgga tatcgttttc
     4561 tacaaagtgc tgattgcact ggagatgttg ttacctgttg gtctgtatgt gtcagttggc
     4621 gtgacgctag ggcagatgta taccgactcg gaaattaccg ctatctctgc ggcggggggc
     4681 agtccgggac gcttgtacaa agccgttctt tatctggcga taccgctaag tatttttgtc
     4741 acccttctgt cgatgtatgg tcgtccgtgg gcttatacgc agatttatca actggagcag
     4801 cagtcgcagt cggagctgga tgttcgccag ttgcgggcaa agaaatttaa cactaacgat
     4861 aacggacgaa tgatcctttc gcagacggtt gatcaggata ataatcgcct gactgacgcg
     4921 ctgatttata cttctactgc caatcgaacc cgcattttcc gcgcccgttc ggttgatgtg
     4981 gttgacccat cacccgagaa accgaccgtt atgttgcaca acggcactgc ctatcttctc
     5041 gatcatcagg ggcgtgacga caacgaacag atctaccgta atctgcaatt acatctgaat
     5101 ccgctggatc aaagccctaa cgtcaaacgc aaagcaaaat cggtcacgga gctggcgcgc
     5161 tccgcctttc ctgccgatca tgccgaactg caatggcgac aaagccgtgg cctgacagca
     5221 ttgttgatgg cgctgctggc catttcatta agtcgggtaa aaccgcggca agggcgattt
     5281 tcaacattat tgccactgac gttgctgttt attgccattt tttatggcgg cgacgtctgc
     5341 cgtacgctgg tggctaacgg cgcgattccc ctcattcctg gtttgtggtt agtacccgga
     5401 ctcatgctaa tgggcctgct gatgctggtc gcacgcgact tctctttgct gcagaaattt
     5461 tcccgatgaa tgttttcagt cgctatttaa tccgtcatct ctttctcggt tttgccgccg
     5521 ccgcagggct attgctgccg ctttttacca ccttcaacct gattaacgaa ctggatgatg
     5581 tcagcccggg cggttatcgc tggactcagg cggtgctggt ggtgctaatg accttaccgc
     5641 gcacactggt cgaactttcg ccgtttatcg cgttattggg tggtattgtt ggactggggc
     5701 agttatcgaa aaacagtgag cttaccgcca ttcgcagcac ggggttttct atcttccgta
     5761 ttgcactggt ggtgctggtt gccgggatat tgtggactgt ttcgttaggc gcgatagatg
     5821 agtgggtggc gtcgccattg cagcaacagg cgttgcaaat caaatcgacc gccaccgcgt
     5881 tgggggagga cgatgacatt accggcaata tgctgtgggc caggcgtggt aatgaatttg
     5941 tgacggtgaa atcgctgaac gagcagggcc agccggtggg cgtggagata tttcattatc
     6001 gcgacgatct ctcgcttgaa tcctacattt atgcacgcag tgccaccatt aaagacgaca
     6061 aaacgtgggt cctgcatggt gtgaatcata aaaaatggct gaatggcaaa gaaacgctgg
     6121 aaacattaga taatcttgcc tggcaatcgg ccttcaccag tatgaatctt gaagagttat
     6181 caatgccggg gaatactttt tctgtccgtc agcttaatca ttacatccat tatttgcagg
     6241 aaaccgggca acccagcagc gaataccgcc ttgcactgtg ggaaaaactg gggcaaccca
     6301 tcctgaccct ggcgatgatt ttgctggctg tgccgtttac ctttactgcc ccgcgttcgc
     6361 ctgggatggg tagccgtctc gctgtaggcg tcatcgtggg cttactcacc tggatcagct
     6421 atcaaatcat ggttaatctg ggattgctat ttgcgttaag cgcacctgtt accgcgctcg
     6481 gtttaccggt agcgtttgtg ttggtggcgt tgagcctggt gtattggtat gacagacaac
     6541 attaaacccg ccgtggcgca aacaccacgg cgggctcaca ttactgcttc agagtagcaa
     6601 atgcctgaat gatggcattg atttgctcac gactgtgggc agcatttacg ctacagcgca
     6661 gcagcgtgat gcctgcaggc gcggcaggcg gcaggataag attcacatac acgcccagcg
     6721 agataagctc gcgccagatg cgtaaacctt cctctttcga accgattatt actggcacta
     6781 ccgggctaat atgtgaactt aactcataac ccagtttttg cagaccgtca tacagatggt
     6841 tggcgttatc catcagcttt tgccgcagct ctggttgtgt ggcaatagtt cttaacgacg
     6901 agcgcacggt cgcaatgcag gacggagaag gagaggcggt aaaaatatac gggcggctgc
     6961 tgtagcgcag cacttccatc gcttccgaac cgacagcaaa accgccaatc gaggccagac
     7021 ttttactgaa agtaccaacg ataatatcca catcatcttc aacgccgaca gcttcagcca
     7081 gtccgcgccc cgttgcgcct aacacgccaa acgaatgcgc ttcatcgaca atcaaataac
     7141 caccgagacg acgtttgata tcaacaattt ccgccagcgg cgcaacgtca ccgagcatgc
     7201 tgtagatgcc ctcaacgatg ataatcgcct ctttcgcccg ttcaccgagg cgaaccatgc
     7261 gccgttccag atctttagca tcgttatggc ggaaacgaat aatttccgcg ccaccgagag
     7321 aacaggcgtc ataaatactg gcgtggctgt cggcatccag taacaccacg gcgttatggt
     7381 cggcaagtgc gctgatgacg cctaaattag cggtataacc ggttgaaaaa acgatagcgg
     7441 tagggcgatt gaaaaactcg gcaatctctt tctctaacgc caggtgcggc gcataactgc
     7501 cgtttgccat acgagaaccg gtggtgccgg tgccttgggc cgctaacgct gcctgacctt
     7561 cgctgatggc atcatgatta aaggttaaac cgagataatt attggtcccg gccagaatca
     7621 cttttttatt gccgatccgg ccttcggtgg cggaatacac ttcatcaata caggtgccaa
     7681 acggtgttaa cccgttgtca ctaaattgca gtcgctcgcc agcaaggcgt gcatatttat
     7741 cgtatagccc cattatttat tctccagcca tggaagtaga gtttccatta attgcgcagg
     7801 ggttttgaca tccagcagaa tattaatagg aatggaaata tcgaatctgt cttccagcat
     7861 cattaagaga tccatcactt tgatcgactc caggccaaga tcgttgacga gatcgctgtc
     7921 gggcttaatt tccacaccgt tttcgaccaa atcctgtaaa caggaaagga tgtaatccat
     7981 tactatttca cgatttacca tagaaacgtt aacacacctt taaacaagac aatcaaaata
     8041 gcccgttgcg taatgcgtgt tccaggctaa tcccgggaaa ccaattaata tcttcagaaa
     8101 tacggttatt acttgccgac cagtcggcgt gggttaattc acgaattttc gagcgggtca
     8161 gcataggttc tttacctgcc aggcgactca acgcggtact gatatccgca aggcaggtga
     8221 gtaccggcag aggaatacca accattcgca cggaaccgca acggacgttg gcggcaagtt
     8281 gctgtatgcg ttgccagtca tagccgccag cgacgctatc gcataattca taggtttgcg
     8341 tctgtacagt ttcggcgctt aaccactgac ccactgcttg cgcaaaatcg gtgacgtgca
     8401 ggaaagagag ctgtgtatct ggtgcaccaa gtcgtggcag caggccgcgc agcatccagt
     8461 caaacagcgg ttttaactct ttatcgccgg gaccatacac ggctgtcggg cgaaaaacgc
     8521 ccagcgtaat ttcgtcagcc attgcagtca gccgttgttc ggcgatgtgt ttggaatttg
     8581 cgtaccagga gagctcggga tggcgcgccg ccagcgaaga gataaacaga aaacgttggc
     8641 aaaagcagct ctcttttgct gcttgcatca ggcgcaggct gccgtcaacg ttacagcggg
     8701 tgaaaatctc ttctttgtgc ccgcgcactt gcccggcgca atggaccact gcgctggctc
     8761 cggcaaccag cttgctaagt gaatgagtat cttccagcga accgcgcacc caggtgagat
     8821 tatcattgac gtgagcgcgg gcagtacgcg tcaatgcgcg aacgtgaaag ccgcgggcga
     8881 gcaggttatc gataatatat ttaccgataa acccggtagc gcccgtcacc gcgacagttt
     8941 gattcatgcc agggattcct gcacattaag actggcagca taagccttct gataacgttt
     9001 tttcgcttcc gcacgggcag gcttgccgga ggacgttcgg ggaatactgt ggggcggcaa
     9061 cagatcgata gccgcggtca cgccaaattc gctttggatc cgtgccgcca gcgcgtggat
     9121 aagctgcccg cgacgttctt cgtcgctgat ccgacactgg atctgcaaaa tgattttttc
     9181 ctgggcggta acaaaagcaa tcgcatcgcc agaatgaatt tccggttctt gttccgcaat
     9241 atattcaata tcctgcggcc agatattacg gccacgaata ataatcagat ctttaatgcg
     9301 tccggtgaca tacagataac cgtccagcag ataaccgagg tcgccggtgt ctaaccagcc
     9361 cgtcgcggca atctcgtctt gcgaaacctg gtcgccaaag taaccgctca tcaggctggg
     9421 gccggagatg caaatatggc ctaccacacg ttccgcgacc ggcatacctg cttcattgcg
     9481 gatttcaatg ccatgttccg gcaacgcttt gccgcagttg acgaaagtcg atacggcgcg
     9541 tgtctctgca cccggcgcga cggctttacc ctgatattcg aggatgtcgc gatccacttc
     9601 gttaaccaca accccggagg cttcatcaga gaagctgaca gccagcgcat tttctgccag
     9661 tccgtagcac ggcatgaaag ttttattgtc aaagttaacc tgacggaaac attcagcgaa
     9721 ttgatggagt tgttctgcgg agatgggttc tgcaccaata ccagcgacgc gccagcagga
     9781 aagatccagt tcagcgagat ctttttcatt cacgcggcgc tggcacaatt catagccaaa
     9841 cggcggcgca acggaaacgg tgccgcgatt tttactgatc aatttaagcc attgcagagg
     9901 acgcatggca aaatcctgag tgcgcaaata atctactgaa agctgcgtgg cgacgggggg
     9961 caggagaaag ccgaccagtc ccatatcatg gtagaaaggc agccaggaga cgcagcggtc
    10021 gccagggcgt aatttaatgc cgtcgtggct tatagcacgt agattagcca tcacttcgcg
    10081 atgggtgata atgacgccac ggggaaaacg ggtgctgccg gaggtgtact ggaggtaggc
    10141 gatatcgttc ggaactggac gctggagcgc aacatcggct tccggtaatg ccttaaacca
    10201 ggcgtggctt aaaacatgta attcggggtt gttatgcgtc gcggcattga ccagtggcaa
    10261 ccactcatca ccagtgataa tggctgcggg ctggcaactt gccagtaaac cctggagttt
    10321 ggcgctccag gaatcccgct gaccgacgcc cattggaatc gccaacggga cggcgactaa
    10381 gccagcatac tgacaggcaa aaaaagcctc tacaaacccg ctactcgttt cggcaatcag
    10441 cgccacgcgt tcgccttttt tcaggttcag cgataacaac cgcttcgcac cagcttcggc
    10501 acgcgttttt aatgtctgat attccagttg atcttcaagt tggcaacgtc tgtcataaaa
    10561 attcattccg gcgctactca gagcggcgta gtccaaagca tcaaccagcg ttggaaaatc
    10621 ggcatagcgc ataggtaggg aatgcgtaaa gattttatta gacatataca ccacgaaaaa
    10681 attggatatc
//
LOCUS       DAZGOB010000151        10662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGOB010000151 DAZGOB010000000
VERSION     DAZGOB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.151"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            311..400
                     /locus_tag="ACHLIF_004303"
                     /pseudo
     CDS             311..400
                     /locus_tag="ACHLIF_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            874..975
                     /gene="ykgR"
                     /locus_tag="ACHLIF_004304"
     CDS             874..975
                     /gene="ykgR"
                     /locus_tag="ACHLIF_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO9608199.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            1339..1602
                     /gene="ykgM"
                     /locus_tag="ACHLIF_004305"
     CDS             1339..1602
                     /gene="ykgM"
                     /locus_tag="ACHLIF_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO9608200.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVTSEGNVARFTQRFGRFVSTKKGA"
     gene            1602..1742
                     /gene="ykgO"
                     /locus_tag="ACHLIF_004306"
     CDS             1602..1742
                     /gene="ykgO"
                     /locus_tag="ACHLIF_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO9608201.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1777..2004)
                     /gene="ykgL"
                     /locus_tag="ACHLIF_004307"
     CDS             complement(1777..2004)
                     /gene="ykgL"
                     /locus_tag="ACHLIF_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFO9608202.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            2826..3368
                     /gene="ecpR"
                     /locus_tag="ACHLIF_004308"
                     /gene_synonym="matA"
     CDS             2826..3368
                     /gene="ecpR"
                     /locus_tag="ACHLIF_004308"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO9608203.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            3442..4029
                     /gene="ecpA"
                     /locus_tag="ACHLIF_004309"
                     /gene_synonym="matB"
     CDS             3442..4029
                     /gene="ecpA"
                     /locus_tag="ACHLIF_004309"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO9608204.1"
                     /translation="MKKKVLAIALVTVFTGIGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            4087..4755
                     /gene="ecpB"
                     /locus_tag="ACHLIF_004310"
                     /gene_synonym="yagY"
     CDS             4087..4755
                     /gene="ecpB"
                     /locus_tag="ACHLIF_004310"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO9608205.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            4781..7306
                     /gene="ecpC"
                     /locus_tag="ACHLIF_004311"
                     /gene_synonym="yagX"
     CDS             4781..7306
                     /gene="ecpC"
                     /locus_tag="ACHLIF_004311"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO9608206.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAEVTQTGEENES"
     gene            7296..8939
                     /gene="ecpD"
                     /locus_tag="ACHLIF_004312"
     CDS             7296..8939
                     /gene="ecpD"
                     /locus_tag="ACHLIF_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO9608207.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            8908..9618
                     /gene="ecpE"
                     /locus_tag="ACHLIF_004313"
     CDS             8908..9618
                     /gene="ecpE"
                     /locus_tag="ACHLIF_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO9608208.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            9931..10260
                     /gene="ykgJ"
                     /locus_tag="ACHLIF_004314"
     CDS             9931..10260
                     /gene="ykgJ"
                     /locus_tag="ACHLIF_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO9608209.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(10508..>10662)
                     /locus_tag="ACHLIF_004315"
     CDS             complement(10508..>10662)
                     /locus_tag="ACHLIF_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HFO9608210.1"
                     /translation="PPLFDLPWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEI
                     PLGSNR"
BASE COUNT         2906 a         2608 c         2526 g         2622 t
ORIGIN      
        1 tagataccta agttatattt cttactctgc ggacagtgaa taattcacta ttcagagtga
       61 ataacaatga cctgccttta attaaaacta ttgaaaatac gtcgaaatag gtatatatgc
      121 caggataaac caatatattt tttatggata ttcattttcc ttcatcggac tgtaaattgc
      181 ggagatatcg aacttgcact acccatcgca acactcaaac agatttatga atttcgttca
      241 taacccctga caaattctgc cgtctaatcg agcttttccc tttacgttat gcttctcatg
      301 aacgataaca caacttgttc atgaattaac cattccggat aaactatggg atcaaattca
      361 ggcatcgaaa ggaactggct ggtttccgtt aatggattaa atatctatta ttctcccgag
      421 agtaaaacta tcaaatcaat gagtaaccga tacagcctca caaattcttt cagataatta
      481 acccgtcact gcggcacgtt ctctcataag gcgtgcctgc agtgtaatcc agtatgtatc
      541 cggcatagtt gacggtttaa tagaactcaa ttcctttccc aggttaccaa caacgatatc
      601 ttgtataaca ccccaggaga tacgacatta tgatgaaatg aggtatgctc ctcagcgcca
      661 atcaacgccc ggactgggcc agtcttgaga tcagactcat tcccggaact gacttcagtc
      721 gacatatatc aatgacgttg gtgcaataaa agatagctga tgtaatacat ctattcaaat
      781 ttgtgataca tgtcaaaaaa ttggttgacc aaactcgtta tttatataag ggcacttacg
      841 aagtgcactc ttttttaaag cgaggaagta ccaatgaaag agaataaagt acaacaaatc
      901 agtcataaac tgattaatat cgttgttttt gtcgcaattg tagaatacgc ctatttattt
      961 ctccatttct attaataacg gcaataaact gttcacttca gtgatattta aaatatgcat
     1021 cctctccctt tttgtaagta attattatat ccgtgggaga ggaatacacg ctgtcaggta
     1081 atcaatcata ctgcgataaa tcatcggcca gtaaagtgga gataacctcc attctcgaaa
     1141 aatccatact ctcagcaaaa ccatcatcaa tcactcatcc aggcgtttat gggagcgtcg
     1201 ccaacggctg ctaacaatgc cagacttccc cgttggggaa attccacata ccacaaatgg
     1261 gcacagggat tgggtgttga aatgatccgg atgagcatgt atctttacgg ttatgttata
     1321 acataacagg taaaaatgat gaagcccaat atccatcctg agtatcgtac tgtggtgttc
     1381 cacgacacca gtgttgatga gtactttaaa atcggctcga ctatcaaaac agaccgtgag
     1441 attgagctgg atggcgtaac gtatccgtac gtgacaattg atgtctcttc taaatcgcac
     1501 ccgttctata cagggaagct gagaacagtg acatcagaag gaaatgttgc acgattcacc
     1561 caacgttttg gtcgttttgt tagcacgaaa aagggggcgt gatgaaagtt cttaactctc
     1621 tgcgtaccgc aaaagaacgc catccagact gtcagattgt gaagcgaaaa ggacggttgt
     1681 atgtgatttg taaatctaat ccacgtttta aggccgttca gggtcgtaag aaaaaacgtt
     1741 gattcaaaat tcgacgaatt aacgatattt gtctgattaa taatcagatc ggattaatgt
     1801 tggtgtgttt ataacaccaa cattaatttt cctggggata tattcttcct gttcatttga
     1861 ggccaactgc ctgacgtttc tctccgaata ttccattatc ttaatgttga cttgttgacc
     1921 agcttcgccc ctgtatgctg gcatcaaccc tcttttagac tgaacacgcc actcagtctc
     1981 ctccctttgc ggcgcagcct gcattttcac tcaaactgtt aagatgataa atgtggtaaa
     2041 tctgttggta ctaacataaa aacgtttacg ccacaggaac agtctgatcc accggtaacc
     2101 cgtagccgac gttcgagtgc cagttagagt aacgcgcaca gataactgca ctcagtgccc
     2161 tgacaaagag gccatcgttc ctgtgacagc tggcagcctt cgtttaactt cacttaatct
     2221 ggctcttggg ggcttaccga acagatgacg tacatacgcc cgttcaattt tccatcactt
     2281 attgtaatga acacctataa ccattttgtg cggcatgtta atccattaaa atttcttact
     2341 gattggcaaa tcatcttcaa tgacagctca ttatagtttt atattctatc ccttaccctt
     2401 aaaacttgtt tttttactag tccatcacac agcgcattaa gactattcct aacacttcag
     2461 ggcaaagttc ctgaccaata taaaatgcaa gtaagaattg aacgttatat tgccaataac
     2521 cttatgaaac caaatgtctt tttcttctta tcaaaaaagc aatattttca gtttttctaa
     2581 atattgactt aaccattgaa ttccttttcc gttcacatat tgacactcat cgggaaaaaa
     2641 acataaattt aagcccaatc gaaaataatt aaacttaatc tcgtttaacc tttattgata
     2701 tgtactacgt atcttattta cttccggttt actaacgaaa ctgaatgcac ctgtaaaaat
     2761 tacaggtttg gaaagtagtg acatggcaaa gtgattacag cagggactat gaggttaaaa
     2821 accatatgga atgtcaaaac cgttctgata aatacatctg gtctccccat gacgcctact
     2881 tctataaagg actatctgaa ctgattgtgg atatcgacag attaatttat ctatcgttgg
     2941 agaaaattag aaaagatttc gtgtttatca atctcaatac ggattcttta tctgaattta
     3001 taaaccgtga taatgaatgg ttatccgcgg taaaggggaa acaggtcgta ttgattgcgg
     3061 ccagaaagtc agaagcctta gcaaattatt ggtattacaa cagcaatatt aggggcgtgg
     3121 tatacgcagg actgagtcgt gatattagaa aagaactggc ctatgtgatt aatggcaggt
     3181 tcctgagaaa agatattaag aaagataaaa tcacggaccg ggaaatggaa attatccgca
     3241 tgacggccca gggaatgcaa cctaaatcga ttgccagaat tgaaaattgt agtgtgaaga
     3301 cagtgtatac ccatcggcgt aatgctgagg ccaagctgta ctcaaaaata tataagttgg
     3361 ttcagtaaac tccaggctag ttagttttaa aaatgactca ctgggacatc acgtcctcaa
     3421 ttcaactcgg gaagaaatgc aatgaaaaaa aaggttctgg caatagctct ggtaacggtg
     3481 tttaccggta taggtgtggc gcaggctgct gacgtaacag ctcaggctgt agcgacctgg
     3541 tcagcaacag ccaaaaaaga caccaccagt aagctggttg tgacgccact cggtagccta
     3601 gcgttccagt atgccgaagg cattaaaggt tttaactcac agaaaggtct atttgacgtg
     3661 gctatcgagg gtgactcaac ggctaccgcc tttaaactga cctcacgtct tatcaccaac
     3721 acattaaccc agttggatac ctcaggttcc acactgaatg tgggcgtgga ttataacggc
     3781 gcggcagtcg aaaaaactgg cgataccgtg atgatcgata ccgccaacgg cgtactgggc
     3841 ggcaacctta gcccgctggc aaacggttac aatgccagca atcgtaccac cgcacaggat
     3901 ggtttcactt tctccatcat cagcggcacc accaatggta ccaccgcagt aaccgattac
     3961 agcactctac cggaaggcat ctggagcggc gacgttagcg tacagttcga cgcgacctgg
     4021 accagttaat ctctctgatg taccagcagg ggtagccccc ctgctttatt cctggacgga
     4081 attgttatga aaaagcacct tctgcctctc gctctgctgt tttccggaat atctccggcc
     4141 caggcgctgg atgtcggcga tatatcatcg tttatgaaca gtgacagcag cacgctgagc
     4201 aaaacgatca aaaacagtac cgacagtggt cgccttatca atatccgtct cgaacggctc
     4261 tcttcaccgc ttgacgacgg gcaggttatc tcaatggaca agccggatga gttgctactc
     4321 actcccgcca gcttgctgct acccgcccaa gccagcgaag tgatccgctt cttctataag
     4381 ggacccgcag atgaaaaaga gcgctactac cgcattgtct ggtttgatca ggccctcagt
     4441 gatgcgcagc gcgataatgc caaccgcagc gctgtggcca ctgcttccgc ccgcatcggc
     4501 accattctgg tcgtcgcccc tcgtcaggcg aactaccact ttcagtacgc caacggctcc
     4561 ctgacaaata caggaaatgc gacgctgcgg atcctcgctt acggcccctg cctgaaagcc
     4621 gccaatggta aggagtgtaa agagaattac tacctgatgc caggcaagtc gcgtcgtttt
     4681 acccgcgtgg acacggcgga taacaaagga cgggttgcac tttggcaggg tgataagttc
     4741 attcccgtga aatagatagc tgtgcagatg gataacgaca atgcctttac gacggttctc
     4801 cccaggactg aaagcccagt ttgccttcgg catggtcttt ttgttcgttc aacccgatgc
     4861 cagcgctgct gacataagtg cgcagcaaat aggtggggtg attattccgc aggccttcag
     4921 tcaggcgctt caggacggca tgagcgtccc gctctatatt catctcgccg gtagccaggg
     4981 tcgccaggac gatcagcgaa tcggcagcgc ttttatctgg ctggatgatg gacagctacg
     5041 catccggaaa atacagctgg aagagagtga agataacgcc agtgtcagcg aacaaactcg
     5101 acagcagctg atggctctgg cgaacgcccc gttcaatgag gcccttacca tccccctgac
     5161 tgacaacgcg cagctggatc tcagcttgcg ccaactgctg ctgcagctgg tggtcaagcg
     5221 cgaagcgctg ggcaccgtac tacgctcacg tagcgaagac atcgggcagt ccagtgttaa
     5281 caccctcagc agtaatctga gctataactt gggcgtctat aacaaccagt tgcgtaacgg
     5341 cgggagcaac acatccagct atctgtcgct gaataacgtt actgcgctgc gcgaacatca
     5401 tgtggtgctc gacggctcgc tgtacgggat cggtagcggt caacaggaca gtgaattata
     5461 taaagcgatg tatgaacgcg attttgccgg tcaccgattt gccggtggaa tgctcgacac
     5521 ctggaacttg cagtccttag ggccgatgac cgccatttca gcagggaaga tttacggcct
     5581 ttcctgggga aaccaggcca gctccaccat cttcgacagc agccagtcag ccacgccagt
     5641 gatcgccttt ttaccggcgg cgggcgaagt acatctcacc cgtgatgggc ggctactaag
     5701 cgttcagaac ttcactatgg gcaatcatga agtggatacc cggggtctac cgtacgggat
     5761 ttacgatgtg gaagttgagg tgatcgttaa cggtcgcgtg atcagcaaac gcccccagcg
     5821 ggtcaataag ctgtttagcc gggggcgcgg cgtcggtgca ccactggcgt ggcaggtatg
     5881 gggcggtagc tttcatatgg atcgctggtc ggaaaacggg aaaaagacgc gaccagctaa
     5941 agagagttgg ctagcaggtg cctcgacctc cggctcattg agtacgctta gctgggcggc
     6001 aacgggatat ggatacgata atcaggcggt gggtgaaacc cgtctgacgc tgccgcttgg
     6061 gggggcgatc aacgttaacc tgcaaaatat gctggccagt gacagctcat ggagcagcat
     6121 cggcagcatc agcgccactc taccgggagg ctttagttcg ctgtgggtta accaggaaaa
     6181 aacccgcatt ggcaatcaat tgcgacgtag cgatgccgac aaccgtgcaa tcggcggcac
     6241 actcaacctg aactcactgt ggtcgaagct gggcacattc agcatcagct acaatgatga
     6301 ccgccgttac aacagccatt attacacggc agattactat caaaatgtct acagcggtac
     6361 ctttggttcg cttggcctgc gggccggtat tcagcgctat aacaacggcg acagcaacgc
     6421 caatacaggg aaatatatcg ctctcgatct ctcgctacca ctgggcaact ggtttagcgc
     6481 agggatgact catcaaaacg gctacaccat ggcaaacctg tcagcacgca aacagtttga
     6541 tgaaggaacc attcgcactg ttggtgccaa tctgtcacgt gccatctccg gcgataccgg
     6601 tgatgacaaa accctcagcg gtggggcgta tgcacagttc gacgctcgtt acgccagcgg
     6661 aacgctgaac gtcaatagcg cggcggacgg ctacatcaat accaacttga ccgccaatgg
     6721 cagcgtcggc tggcagggta aaaacatcgc tgccagcggg cggactgatg gcaacgctgg
     6781 ggtgatattc aacaccgggc tggaggacga cggtcagatc agcgccaaaa tcaacgggcg
     6841 gattttcccg cttaacggca agcgtaacta tctcccgctc tctccctatg gaagatatga
     6901 ggtggagtta cagaacagca aaaactcact cgacagctac gatatcgtca gcggccgcaa
     6961 aagccatctg actctctatc caggcaatgt cgctgtcatt gagccagagg tgaagcagat
     7021 ggttaccgtc tccggtcgta tccgtgcgga agacggcaca ctgctggcta acgcacggat
     7081 taacaaccat atcggccgaa cccgaaccga tgaaaacggc gagtttgtca tggacgtgga
     7141 taagaaatat cccactatcg attttcgcta cagtggcaat aaaacctgcg aagtggcact
     7201 ggaactcaac caggcgcgcg gtgccgtctg ggtcggtgat gtggtctgca gcggcctctc
     7261 atcgtgggcg gaggtgacgc agacaggaga agagaatgag agttaaccta ctaataacga
     7321 tgataatttt cgcgctaatc tggccagtaa ctgagctcag agcggcagtg agcaaaacaa
     7381 cctgggcgga tgcaccggca cgcgagtttg tgtttgtcga aaacaactca gacgacaact
     7441 ttttcgtcac tcctggcggg gcgctggatc cgcgcctgac cggtgccaac cgctggaccg
     7501 gtttaaaata cactggttca ggaaccatct atcagcaaag cctcggctac attgataacg
     7561 gttacaacac cggcctttat accaactgga agtttgatat gtggctggaa aattcaccag
     7621 tttcatctcc tttaactggc ttgcgctgca tcaactggta cgctgggtgt aatatgacca
     7681 ccagtcttat cctgccgcaa accaccgacg ccagtggatt ttatggcgcg acggtgacca
     7741 gcggcggcgc gaagtggatg cacggcatgt tgtcagacgc gttttaccag tatctgcaac
     7801 aaatgcccgt cggcagcagc tttacaatga ccatcaatgc ctgccagacc tctgtgaact
     7861 atgacgccag cagcggcgca cgctgtaagg atcaggcctc cggcaactgg tatgttcgca
     7921 acgtcaccca tacgaaagca gcaaatctgc ggttaataaa tacccactcg ctggcggagg
     7981 tatttatcaa cagcgacgga gtaccgactc tgggcgaagg gaacgccgac tgccggacac
     8041 agaccatcgg cagcctttca ggattaagtt gtaagatggt caactatacc ctgcaaacaa
     8101 acggactcag caacacctca atccatatat tcccggcgat cgccaactcg tcgttagcct
     8161 cggccgtcgg ggcgtacgat atgcagttca gtctgaatgg cagttcatgg aaaccggtga
     8221 gcaataccgc ctattactac accttcaacg agatgaagag cgcagactcg atctatgttt
     8281 tcttctcgag caacttcttt aagcagatgg tgaacctcgg gatcagcgat atcaacacca
     8341 aagatctatt caactttcgc tttcagaaca ccacatcacc ggagtctggc tggtatgaat
     8401 tttctacctc caacacgctg attatcaaac cccgtgattt cagcatcagt attatctccg
     8461 atgaatatac tcagacaccg tcgcgggagg gatatgttgg cagcggcgag tcggcactcg
     8521 atttcggcta tatcgtaacc accagcggta aaacagctgc cgacgaagtg ctgatcaagg
     8581 tgaccggacc cgcgcaggtg attggcgggc gctcctattg tgtcttcagc tccgatgacg
     8641 gtaaggcgaa agtaccgttc ccggcgacac tttcctttat tacccgcaac ggagctacaa
     8701 aaacctacga tgccgggtgc gatgatagct ggcgggatat gaccgatgcg ctgtggttga
     8761 ccacaccgtg gactgatatc tctggcgaag tggggcagat ggataagacc acagtcaaat
     8821 tttcgattcc aatggataac gccatttcgc tgcgtacggt agatgataac ggctggtttg
     8881 gcgaagtcag cgcttcagga gagattcatg ttcaggcgac gtggcgtaac attaactaag
     8941 gccctgctga cagcggtctg tatgctggcg gcacctttga cacaggcgat ttcggtcggc
     9001 aatctgacat tttcgctgcc gtccgagact gactttgtca gcaaacgtgt agtgaataac
     9061 aacaaaagcg cacggatata ccgtattgcc atcagtgcta ttgatagccc gggcagcagt
     9121 gaattgcgca cccgaccggt ggatggtgaa ctgctttttg ccccccgcca actggcgttg
     9181 caggctggtg agagcgagta ttttaaattt tactatcatg gtccacggga taaccgcgag
     9241 cgctactacc gggtctcatt tcgcgaagtc cccactcgta accagacaag acgtagccca
     9301 accggcggcg aggtcagcac ggagccggtg gtggtgatgg ataccattct ggtagtacga
     9361 ccacgtcagg ttcagtttaa atggtccttc gacaaggtga caggaacggt aagtaacact
     9421 ggcaacacat ggtttaagct actgattaag ccaggatgtg attcgaccga agaggaaggc
     9481 gatgcctggt atctacgtcc gggagacgtg gttcatcagc ctgagttacg tcagccgggg
     9541 aatcattatc tggtctataa cgacaaattc attaagatta gcgattcctg tccggctaag
     9601 cccccttcgg cggactaaga cttatccatc ggtcgaggga agaattccac ctcagagctc
     9661 caaaaatcgt ttaaatgatg tggaagcgat cgtcaatgtc acctgtaacg atgggctttc
     9721 tcatatgcac accatagtgt tcaggagaca cggaaaggta tcaggcagcc tgctaatgag
     9781 tggtttaatc acgaattagt acgtaaaatc ggtaacggct ggaaatcatt caatactcac
     9841 actatcgaaa gttccccagc caaccgcggt acgttcttac atacgatgta ccgctgttct
     9901 ctttacgatt tttagctgta ctggtgaatt atgagcaatc tgaatccatg catgacgtgt
     9961 ggtgcctgtt gtgcattttt ccgcgtctct ttttactggg ccgaagccga cgatgctggc
    10021 ggtactattc ccgccaggct cactgaacaa atatcccctt ttcaccgatg catgagcggt
    10081 accaatcaga aaaacccccg atgtattgcc cttgcaggaa ccccgggcaa aaatgcctgc
    10141 tgcacgatat ataaaaatcg atcgtccaca tgcagagaat ttgccatgtc tggtgagaac
    10201 ggagtcgtca atgaggcttg caatcgtgca agggctaaat acgggctgac accgttataa
    10261 acatacaata attaattgca ctgccccgcc agcgataata gcggggcttc gtttttcagg
    10321 ggtaacaaaa cccgatactt cttctatttg ccagcaacaa tgcctctctt ctgtagcgtt
    10381 cttcggatct acatcatcct gaagtagcgc gatatactca ctgactatct gaatcagaat
    10441 ataaaaagca atgtttttaa cctataaaaa tggcgctgta tttgcgccat ttttatcatt
    10501 caatgcatta tctgtttgag cctaaaggga tctcagggtc tggctcatga gtaattctgt
    10561 ttcgtaaatc tctgcgaata atttcaatag accagaacca gactaaatgt ccaaaaattt
    10621 cagaaacatt ctcataccac gggagatcaa acagaggtgg cg
//
LOCUS       DAZGOB010000152        10314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGOB010000152 DAZGOB010000000
VERSION     DAZGOB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.152"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            17..592
                     /gene="ssuE"
                     /locus_tag="ACHLIF_004316"
     CDS             17..592
                     /gene="ssuE"
                     /locus_tag="ACHLIF_004316"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO9608211.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            585..1544
                     /gene="ssuA"
                     /locus_tag="ACHLIF_004317"
     CDS             585..1544
                     /gene="ssuA"
                     /locus_tag="ACHLIF_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO9608212.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGAVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAKVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1541..2686
                     /gene="ssuD"
                     /locus_tag="ACHLIF_004318"
     CDS             1541..2686
                     /gene="ssuD"
                     /locus_tag="ACHLIF_004318"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO9608213.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2697..3488
                     /gene="ssuC"
                     /locus_tag="ACHLIF_004319"
     CDS             2697..3488
                     /gene="ssuC"
                     /locus_tag="ACHLIF_004319"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO9608214.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3485..4252
                     /gene="ssuB"
                     /locus_tag="ACHLIF_004320"
     CDS             3485..4252
                     /gene="ssuB"
                     /locus_tag="ACHLIF_004320"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO9608215.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4459..7071)
                     /gene="pepN"
                     /locus_tag="ACHLIF_004321"
     CDS             complement(4459..7071)
                     /gene="pepN"
                     /locus_tag="ACHLIF_004321"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO9608216.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7337..8539
                     /gene="pncB"
                     /locus_tag="ACHLIF_004322"
     CDS             7337..8539
                     /gene="pncB"
                     /locus_tag="ACHLIF_004322"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO9608217.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8708..10108
                     /gene="asnS"
                     /locus_tag="ACHLIF_004323"
     CDS             8708..10108
                     /gene="asnS"
                     /locus_tag="ACHLIF_004323"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO9608218.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2316 a         2675 c         2783 g         2540 t
ORIGIN      
        1 gttataagga gagagtatgc gtgtcatcac cctggcgggt agtcctcgct ttccttctcg
       61 ctccagctcc ttgctggaat atgcgcggga aaaactaaat tgcctggatg tagaggttta
      121 tcactggaat ctgcaaaact tcgccccgga agatctgctt tacgctcgtt tcgatagtcc
      181 ggcactcaag accttcaccg aacagctgca acaggctgat ggactgatag tcgctacacc
      241 tgtgtataaa gccgcctatt ccggcgcgct gaaaaccctg ctcgacctgc tgccagagcg
      301 cgctttgcaa ggcaaagtgg tgctaccgct ggcgacgggc ggtacggtgg ctcatctgct
      361 ggcggtcgat tatgccctta agccagtttt aagtgcgatg aaagcccagg agatcctgca
      421 cggcgtgttt gccgatgact cacaagttat tgattaccat cacaaacccc agttcacgcc
      481 aaatctgcaa acccgtcttg ataccgcgct agaaactttc tggcaggcat tgcaccgccg
      541 cgatgttcag gttcctgacc ttctgtctct gcgaggtaat gtccatgcgt aacatcatta
      601 aactggcgct ggcgggattg cttagcgtct ctacttttgc ggttgctgca gaatcctcgc
      661 ctgaagcgtt acgtataggc tatcagaaag gcagtattgg tatggtattg gcaaaaagcc
      721 accagttact ggaaaaacgc tatccgcaat caaaaatctc ctgggtggag ttccccgcgg
      781 gtccgcaaat gttggaagcg ttaaacgttg gcagtattga tctcggcagt accggggata
      841 ttccgccaat ctttgcccag gctgccgggg ctgatttggt atatgtgggc gtcgagccac
      901 cgaagcccaa agccgaagtg attctggtgg cagaaaacag cccgatcaaa accgtagccg
      961 atcttaaagg tcacaaagtt gcctttcaga aaggttccag ttcacacaac cttttactgc
     1021 gtgcactgcg tcaggccgga cttaagttta ccgatatcca acccacttac ctgacgcccg
     1081 ctgatgcccg cgccgcgttc cagcaaggta acgttgacgc ctgggctatc tgggatccct
     1141 actactctgc tgcattatta cagggcgccg tgcgggtgct gaaagacggc accgatctca
     1201 atcaaactgg atcgttttat ctggcagctc gtccctatgc agaaaaaaac ggcgctttta
     1261 ttcagggcgt actcgcaacc tttagtgagg ccgatgcgtt aacccgcagc cagcgcgagc
     1321 aaagcatcgc tttactggca aaaacgatgg gcttaccggc accggtgatt gcctcttact
     1381 tagatcatcg ccctcctacc accatcaaac cggttaacgc caaggttgcc gccttacagc
     1441 agcaaacggc agatctgttt tatgaaaatc gtctggtgcc gaaaaaagtc gatattcgcc
     1501 agcgcatctg gcagcccact caactggaag gaaaacaatt atgagtctga atatgttctg
     1561 gtttttaccg acccatggtg acgggcatta tcttggaacg gaagaaggtt cacgcccggt
     1621 tgatcacggc tatctgcaac aaattgcgca agcggcggat cgtctgggct ataccggtgt
     1681 gctgatccca acggggcgat cgtgtgaaga tgcgtggctg gtggcggcgt cgatgatccc
     1741 ggtgacgcag cggctgaagt ttcttgtcgc cctgcgcccc agcgtaacct cacctaccgt
     1801 tgccgctcgc caggccgcca cgcttgaccg tctctcaaat ggtcgtgcgt tgtttaatct
     1861 ggtcacaggc agcgatccac aagagctggc aggcgacggc gtgttccttg atcatagcga
     1921 gcgctacgaa gcctcggcgg aatttaccca ggtctggcgg cgcttattgc tgggggaaac
     1981 cgtcaatttc aacggtaaac atattcatgt gcgcggagca aaactgctct tcccgccgat
     2041 tcaacagcct tatcctccgc tttactttgg cggatcgtca gacgttgccc aggagctggc
     2101 ggcagaacaa gttgatctct acctcacctg gggcgaaccg ccagaactag taaaagagaa
     2161 gatcgaacaa gtgcgggcga aagctgccgc gcatggacgc aaaattcgtt tcggtattcg
     2221 tctgcatgtg attgttcgtg aaactaacga cgaagcgtgg caggccgccg agcggttaat
     2281 ctcgcatctt gatgatgaaa ctatcgccaa agcacaggcc gcattcgccc ggacggattc
     2341 cgtaggacaa cagcgaatgg cggcgttaca taacggcaag cgcgacaatc tggagatcag
     2401 ccccaattta tgggcgggcg ttggcttagt gcgcggcggt gccggaacgg cgctggtggg
     2461 cgatggtcct acggtcgctg cgcgaatcaa cgaatacgcc gcgcttggca tcgacagctt
     2521 tgtgctttcg ggctatccgc atctggaaga agcgtatcgg gtcggcgagt tgctgttccc
     2581 acatctggat gtcgccattc cggaaattcc ccagccgcag ccgctgaatc cgcaaggcga
     2641 agcggtggcg aatgatttta tcccccgtaa agtcgcgcaa agctaaggaa gaaataatgg
     2701 caacgccagt gaagaagtgg ttattgcgcg ttgccccctg gtttttaccg gtgggcatcg
     2761 tggcggtgtg gcaactggcc tcctcagttg gctggctgtc gacgcgtatt ttgccctcac
     2821 cggaaggggt ggtgacggcg ttctggacgc tctccgccag cggcgaactg tggcagcatc
     2881 tggcgatcag ttcctggcgg gcgctgattg gcttttcaat tggcggatcg ctggggttga
     2941 ttttgggact gattagcggg ctatctcgct ggggagaacg gctgctggat acctcaattc
     3001 agatgttgcg caacgtaccg catctggcgc tgatcccgct ggtgattttg tggtttggca
     3061 ttgatgaatc cgcaaaaatc tttctggtgg cgctcggcac gctattcccc atttatatca
     3121 acacctggca tggcatccgt aatatcgatc gcggtctggt ggaaatggcg cgtagctatg
     3181 gattatccgg cataccgctg tttatccatg tgatcctgcc tggtgccctg ccctcaatta
     3241 tggtcggcgt gcgttttgcg ttgggcctga tgtggctgac gctgattgtt gccgaaacca
     3301 tttctgccaa ttcaggcatt ggttatctgg cgatgaatgc gcgggagttt ttgcaaacgg
     3361 acgtggtggt agtcgctatt attctttacg ccctgctcgg caaacttgct gacgtcagcg
     3421 cgcagctgtt agagcgcctc tggttgcgct ggaacccggc ttatcatttg aaggaggcca
     3481 cggtatgaat actgctcgtc tgaaccaggg cacaccattg ttgctcaatg cagtaagcaa
     3541 acattacgcg gaaaatatcg tcctgaacca actggattta catattccgg caggtcagtt
     3601 tgtggcggtg gtgggccgca gcggtggtgg caaaagtacc ctgctgcgcc tgctggcagg
     3661 tctggaaacg ccaaccgcag gcgatgtgtt agcgggcacc acaccgctgg ctgagattca
     3721 agaagacacg cgaatgatgt ttcaggatgc gcgtctgctg ccgtggaaat cggtgattga
     3781 taacgttggg ttaggcctta aaggccagtg gcgcgatgcc gcgcgtcggg cgttggctgc
     3841 agtaggactg gagaatcgcg ccggggaatg gcctgccgca ctttctggcg ggcagaaaca
     3901 gcgtgtggcg ctggcaagag cgttgattca tcgaccgggt ttattgttgc ttgatgaacc
     3961 gctcggggcg ctggacgcct taacgcgact cgagatgcag gatttgattg tgtcactttg
     4021 gcagcagcac ggctttaccg tactgttggt gacgcatgat gtgagcgaag cggtagcaat
     4081 ggctgaccgg gtgttgttaa ttgaagaggg aaaaattggt ctggatttga cagtggatat
     4141 cccccgtccg cgtcgattag ggtcggtgcg attagcggag ctggaagcgg aagtattgca
     4201 acgggtaatg cggcgtgggc attctgaaca actgattcgg cgtcatggtt aatatgccgg
     4261 atgtggcgca caaaaccagc attttcaacg gactagtagg cctgataaga cacgtcaagc
     4321 atcgcatcag gcagttgggc acggctgccg gatgcggcgt gaacgcctta tccagcctac
     4381 aaaaccagca atttcaacgg agttgtaggc ctgataagac gcgtcaagcg tcgcatcagg
     4441 cattttggct caaaagtatc aagccagtgc tttggtgatc ttctcgtaca gatcgccaga
     4501 gagattttcc agccctttca actgttccag cgccgcgcgc attttctcct gacgtttggc
     4561 atcataacgt ttcaggcgaa tcagcggttc aatcagacgt gaagctacct gcgggttacg
     4621 gctgttgagg tcggtaagca tttccaccag gaactggtaa ccgctgccat cttcggcatg
     4681 gaacgctgcc gggttgctgc ccgcaaacgc accaatcaac gaacgaatac ggttcgggtt
     4741 gctcatggta aatgagcgat gctgcaacag accacgcacc gtctccagca cattcgccgc
     4801 cgggctggtg gcttgcagga taaaccattt atccatcacc agaccgtcct gatgccactt
     4861 atcgtcgtac tcctgcatca gcgcgtcacg gcaaggcagc tgtgcagcaa ccgccgcaga
     4921 aagcgctgcc agcgcatcgg tcatattgtt tgcttcatgg tattgcttgc tcaccagcac
     4981 gtcagccaga tgcgtttcac caaaagcaag gaagcgcagg caggcattac gcagagtgcg
     5041 ttttgcaata tcttcatgct caacacggta ctcgctctgg taattcgcgt tgtaaatagc
     5101 cagcagctca tccgccagtt cagtcgccag agtacgagtg agtgcttcgc gtacttcggc
     5161 aatagcaatc gggtcgatga tatcgaacag ttcagccatt tcattgacag aaggcagcgt
     5221 caggatttct gccgccagcg ccggatcaat cttctcatcg agcagcaccg cgcggaaagc
     5281 gtcagccaca tgtaccggca gagacagcgg ctgcccttgc tgatgacggg cgacgttcag
     5341 cttgatgtag gttgccagca aactttgcgc cgcatcccaa cgggagaaat cattacgcgc
     5401 atgacgcatc aggaaggtca gttgctgatc gctccactta tattccagtt tcactggcgc
     5461 agagaattcg cacagcagcg caggcaccgg ctggaagtag acattatcaa aaacaaaggt
     5521 ctgttccgcc tgggtgacgt tcagcacgga attcaccgga tgaccgcctt tctgcaacgg
     5581 gatcactttg ccttcgttgt catacaattc gatggcaaat ggaatatgca gcggctgttt
     5641 ttctgcctga tccggcgtgg ctggcgtgcg ctggctgatg gtcagggtgt actgctcggt
     5701 ttccggattg tagtcgtctt tgacggtcac aatcggcgtg ccggactggc tgtaccaacg
     5761 gcggaaatgg gagagatcga cattcgacgc atcttccatc gcctgcacaa agtcgtcgca
     5821 ggttgccgcg ctgccatcat ggcgctcgaa atagagttgc atccctttct ggaagttttc
     5881 ttcgcccagc agggtgtgga tcatgcgaat cacttccgcg cccttctcgt aaacggtcag
     5941 ggtgtagaag ttgttcatct caatgaccat atccgggcgg atcgggtgcg ccatcgggct
     6001 ggcgtcttcc gcaaactgca atccgcgcat ggtgcgcaca ttattgatac ggtttaccgc
     6061 gcgggaacca aggtcagagc tgaattcctg atcgcggaag acggttaaac cttctttcag
     6121 gctgagctgg aaccagtcgc ggcaggtcac tcggttaccg gtccagttat ggaaatattc
     6181 atggccgata acgcgttcaa tatcgagata atctttgtcg gtggcggtgt cggtgcgggc
     6241 caacacatat ttggagttaa agatattcag acccttattc tccattgcgc ccatattgaa
     6301 gaaatccacc gcgacgatca tatagatgtc gaggtcatac tccaggccaa agcgttcttc
     6361 atcccatttc atggagtttt tcagcgaggt catcgcccac ggcgcgcgat caaggttgcc
     6421 gcgatcgacg tacagctcca gtgctacttc gcgaccagaa cgcgtggtaa aggtgtcgcg
     6481 cagtacatca aagtcgcctg ccaccagcgc aaacaggtag cacggtttcg ggaacgggtc
     6541 ctgccactgt acccaatggc gtccgttttc cagttcacct tgcgcaacgc ggttaccgtt
     6601 ggaaagcagg aagggatatt tggttttatc ggcaataatt ttggtggtaa aacgcgccag
     6661 cacgtccggg cggtcgagat aatacgtaat atggcggaaa ccttcggctt cacactgggt
     6721 gcaaagcgca tcgcctgact gataaagccc ttccagcgcc gtattcgccg ccgggcttat
     6781 ttcattaatg atcttaagcg taaaacgctc cggcaaatta ctgatgacca gtgcgccctc
     6841 ttcttctttc caggcggtcc acggctcatc attaatatga acagaaacca gtttgaggtc
     6901 ttcgccgttg agacgaagcg gagcatctga tgcaccatga cggacagcct ggctgaccgc
     6961 ggtaacgacc gtcttttgcg cgtcgaggtc aaaggtcaag tcaatatcag taatctggta
     7021 atccggcgca cgataatcgt gacggtattt ggcttgtggc tgttgagtca taaataacct
     7081 ttagcatctt ttatagagtc tggtgttcag tctattcctg ttgcgtaaat cgcgctatgc
     7141 agaatcttca tcttttcagg tacaaacgcc tttattgcta catttttata acatacaccg
     7201 cgtaatgcca tcgaccagaa aggtggcata tggtgtgatc ggggttcaat aaattgcgaa
     7261 acaaggtata ctccagcagt tcctgaagat gtttattgta ctaaacgctc ctgtacgagg
     7321 acgctactgc gcacctatga cacaattcgc ttctcctgtt ctgcactcgt tgctggatac
     7381 agatgcttat aagttgcata tgcagcaagc cgtgtttcat cactattacg atgtgcatgt
     7441 cgcggcggag tttcgttgcc gaggtgacga tctgctgggt atttatgccg atgctattcg
     7501 tgaacagatt caggcgatgc agcacctgcg cctgcaggat gatgaatatc agtggctctc
     7561 tgccctgcct ttctttaagg ccgactatct taactggtta cgcgagttcc gctttaaccc
     7621 ggaacaagtc accgtatcca acgataatgg caagctggat attcgtttaa gcggcccgtg
     7681 gcgtgaagtc atcctctggg aagttccttt gctggcggtt atcagtgaaa tggtacatcg
     7741 ctatcgctca ccgcaggccg acgttgcgca agccctcgac acgctggaaa gcaaattagt
     7801 cgacttctcg gcgttaaccg ccggccttga tatgtcgcgc ttccatctta tggattttgg
     7861 cacccgtcgc cgtttttctc gcgaagtaca agaaaccatc gttaagcgtc tgcaacagga
     7921 atcctggttc gtgggcacca gcaactacga tctggcgcgt cggctttccc tcacgccgat
     7981 gggaacacag gcacacgaat ggttccaggc acatcagcaa atcagcccgg atctagccaa
     8041 cagccagcga gctgcacttg ctgcctggct ggaagaatat cccgaccaac ttggcattgc
     8101 attaaccgac tgcatcacta tggatgcttt cctgcgtgat ttcggtgtcg agttcgccag
     8161 tcgctatcag ggcctgcgtc atgactctgg cgacccggtt gaatggggtg aaaaagccat
     8221 tgcacattat gaaaagctgg gaattgatcc acagagtaaa actctggttt tctctgacaa
     8281 tctggattta cgcaaagcgg ttgagctata ccgccacttc tcttcccgcg tgcaattaag
     8341 ttttggtatt gggactcgcc tgacctgcga tatcccccag gtaaaacccc tgaatattgt
     8401 gattaagttg gttgagtgta acggtaaacc ggtggcgaaa ctttctgaca gccctggcaa
     8461 aactatctgc cacgataaag cgtttgttcg ggcgctgcgc aaagcgttcg accttccgca
     8521 tattaaaaaa gccagttaat atcatcaggg agctaatcgg ctcccttttt ttacctttaa
     8581 ttccgaaatc tttcgctgca tttgcgaatt ctgcttgtct gattgcagat gccaggtaac
     8641 ataggtatcc ccccatttac gggtggacaa gtgtttattt tttccgacta ttaacagaga
     8701 gaatattatg agcgttgtgc ctgtagccga cgtactccag ggccgcgtag ccgttgacag
     8761 cgaagtcacc gtacgcggat gggtacgtac ccgccgagat tcaaaagctg gcatctcctt
     8821 cctcgccgtt tatgacggtt cctgctttga tcctgtacag gctgtcatca ataattctct
     8881 gcccaattac aatgaagacg tcctgcgtct gaccaccggc tgctcggtca ttgtgacggg
     8941 taaagtcgtg gcgtcgccgg gccaggggca acaatttgaa attcaggcca gcaaggttga
     9001 agttgctggt tgggttgaag atccagacac ttacccgatg gcggcaaaac gccacagcat
     9061 tgagtatctg cgtgaagtcg ctcacctgcg tccgcgcaca aacctgattg gtgccgtcgc
     9121 gcgcgttcgt catacgctgg cgcaggcgct gcatcgcttc tttaacgagc agggattctt
     9181 ctgggtttca acgccactga ttaccgcatc tgataccgaa ggtgcaggcg aaatgttccg
     9241 cgtttctacg ctggatctgg aaaacctgcc gcgtaacgat cagggcaaag tggatttcga
     9301 caaagacttc tttggtaaag agtctttcct gaccgtatct ggccagttga acggcgaaac
     9361 ctacgcttgc gcattatcca aaatttatac cttcggcccg actttccgtg ctgaaaactc
     9421 caacaccagc cgtcacctgg cggaattctg gatgctggag ccggaagtgg cgtttgctaa
     9481 cctgaacgat atcgcgggtc tggctgaagc catgctgaaa tatgtcttca aagcggttct
     9541 cgaagaacgc gcagacgaca tgaaattctt cgctgaacgc gtagataaag atgccgtttc
     9601 acgtctggaa cgcttcattg aggccgattt cgcgcaggtg gattacaccg acgcagtgac
     9661 cattctcgaa aactgcggca ggaagtttga aaacccggtt tactggggcg tcgatctctc
     9721 ttctgagcat gagcgttatc tggcggaaga acactttaaa gcaccggtag tggttaaaaa
     9781 ctatccgaaa gatattaaag cgttctatat gcgcctcaac gaagacggta aaaccgttgc
     9841 ggctatggac gttctggctc cgggcatcgg tgagatcatt ggtggctccc agcgtgaaga
     9901 acgtctggac gttctggacg agcgtatgct ggaaatgggc ctgaataaag aagattactg
     9961 gtggtatcgc gatctgcgtc gctacggtac tgttccgcat tcaggtttcg gtcttggttt
    10021 tgaacgcctg attgcttacg taactggcgt gcaaaacgtg cgtgatgtga ttccgttccc
    10081 acgtactccg cgcaacgcca gcttctgatt ttcttcctca aagagccagc gcctgctggc
    10141 tcttttttat cctttctgtc aagttatctg tttgttaagt caagcaatct atttgcaacc
    10201 ccgccataac gatcatcctg ttacggaata ttacattgca acatttacgt acaaaaaata
    10261 acccgcattc ttattgcgga ttagtttttt cttagctgat agcacaattt tcat
//
LOCUS       DAZGOB010000153        10255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGOB010000153 DAZGOB010000000
VERSION     DAZGOB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.153"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..615)
                     /gene="yncG"
                     /locus_tag="ACHLIF_004324"
     CDS             complement(<1..615)
                     /gene="yncG"
                     /locus_tag="ACHLIF_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO9608219.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            881..2380
                     /gene="ansP"
                     /locus_tag="ACHLIF_004325"
     CDS             881..2380
                     /gene="ansP"
                     /locus_tag="ACHLIF_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO9608220.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2495..3556)
                     /gene="yncE"
                     /locus_tag="ACHLIF_004326"
     CDS             complement(2495..3556)
                     /gene="yncE"
                     /locus_tag="ACHLIF_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO9608221.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3798..5900
                     /gene="pqqU"
                     /locus_tag="ACHLIF_004327"
     CDS             3798..5900
                     /gene="pqqU"
                     /locus_tag="ACHLIF_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO9608222.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5936..6601)
                     /gene="mcbR"
                     /locus_tag="ACHLIF_004328"
     CDS             complement(5936..6601)
                     /gene="mcbR"
                     /locus_tag="ACHLIF_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO9608223.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6799..7836)
                     /gene="curA"
                     /locus_tag="ACHLIF_004329"
     CDS             complement(6799..7836)
                     /gene="curA"
                     /locus_tag="ACHLIF_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO9608224.1"
                     /translation="MRQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8017..8535
                     /gene="mddA"
                     /locus_tag="ACHLIF_004330"
     CDS             8017..8535
                     /gene="mddA"
                     /locus_tag="ACHLIF_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO9608225.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8532..8981
                     /gene="ydcZ"
                     /locus_tag="ACHLIF_004331"
     CDS             8532..8981
                     /gene="ydcZ"
                     /locus_tag="ACHLIF_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9608226.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8982..9215)
                     /gene="ydcY"
                     /locus_tag="ACHLIF_004332"
     CDS             complement(8982..9215)
                     /gene="ydcY"
                     /locus_tag="ACHLIF_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO9608227.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9301..9474)
                     /gene="ortT"
                     /locus_tag="ACHLIF_004333"
     CDS             complement(9301..9474)
                     /gene="ortT"
                     /locus_tag="ACHLIF_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO9608228.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9669..9764
                     /gene="yncL"
                     /locus_tag="ACHLIF_004334"
     CDS             9669..9764
                     /gene="yncL"
                     /locus_tag="ACHLIF_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO9608229.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(10166..>10255)
                     /locus_tag="ACHLIF_004335"
     CDS             complement(10166..>10255)
                     /locus_tag="ACHLIF_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HFO9608230.1"
                     /translation="EEMNRLISDGNIQERDYKKFNLSEVFRGL"
BASE COUNT         2481 a         2500 c         2484 g         2790 t
ORIGIN      
        1 aataatttca ttccttttta atacttcttg taatttcggt aactgacata cagcatcagc
       61 gatcgcgctg atattctgcg cattatcctg aaaccattca tgccccggcc cccatgtgcg
      121 catggtgcag agataacagt ccaccagcgt tagttgctca ccaaaggcat aaggttcagc
      181 ggtcaattgt gaattcagcc agatataaag tgatttgcga tattcaatta cattcttctt
      241 taactgttca ggtgcgtcag atgcccaacg ctcagggtaa tcggcgaaag tgaatgttgg
      301 atagacatta gcaaccagcc agaccaatag ccgttgaaac tgctgacgtt cggcgcgccc
      361 aaccggtggc gcaaggtccg ggcgacgatc gagaaccata aatgcaatcg ccgctgtctc
      421 cgtcataatc tcgtcatttt ccagcgccag tgtcggaacc tgacacaacg ggttcagggt
      481 tttcaataac tcgcgtgaag ctccctcgtg gtcaaaacca ctgacatcaa cgaactgata
      541 aggaatatca gccagtgtta gcatcagctc actgattgta gagccccatc cgggtacgcc
      601 atacacctta atcatatgcc ctcacaatct tttaaagatt aagtgtagga cagtacacag
      661 gttaaccagc aaaaagattt gtcatttttt cgtcagttgt attattcccc ttcaggattg
      721 taaggggatg ttttgcatcg tttatcgata acgggtagga tgctgcgccg atagttttat
      781 acccataaag aataatggtg ataactatca tcgccaggat gaataaacat tgttcatggc
      841 aacttatatg actttttcat taaagcaatc agggagagca atgagtaaac acgacaccga
      901 cacttcagat caacacgccg cgaaacgccg ctggcttaat gcccacgaag aggggtatca
      961 caaagcgatg ggcaatcgcc aggtacagat gatcgccatt ggcggcgcga ttggcaccgg
     1021 cttgttttta ggtgcaggag cccgactgca aatggcgggt ccagcattgg cactggttta
     1081 tttaatttgt ggcttgtttt cgttttttat tctgcgtgca ttgggtgagc tggtgctaca
     1141 ccgcccttcc agtggcagtt ttgtctctta tgcccgtgag tttttgggtg agaaagccgc
     1201 ttatgttgct ggctggatgt acttcatcaa ctgggcgatg acggggattg ttgatattac
     1261 cgccgtcgct ctgtatatgc attactgggg tgcgtttggc ggcgtgccgc agtgggtctt
     1321 tgcgctcgct gcacttacca tcgttggcac catgaatatg atcggtgtga aatggtttgc
     1381 ggagatggag ttctggtttg cgcttattaa agtgctcgcc attgtgacct ttttggtcgt
     1441 gggtacagtg ttcctcggta gtggtcagcc gctggatggc aacaccactg gctttcattt
     1501 aattaccgat aatggcggct tcttccccca cggtttgctg cctgctctgg tgttgattca
     1561 gggcgtagtg tttgcttttg cctccattga aatggtgggt acagctgccg gagaatgtaa
     1621 agatccgcag accatggtgc ctaaagcgat taacagcgtg atttggcgta ttggcctgtt
     1681 ttacgtcggc tccgtggtgt tgctggttat gctattgccg tggagcgcgt atcaggcggg
     1741 gcaaagtccg ttcgtgacgt ttttctctaa actgggtgtg ccatatatcg gcagcattat
     1801 gaacattgtg gtgctgaccg ctgccctctc cagcctgaac tcaggtctgt actgcaccgg
     1861 acgtattctg cgctcaatgg cgatgggcgg ttccgcaccg agttttatgg cgaaaatgag
     1921 tcgtcagcat gtgccgtatg ccgggattct ggcgacacta gttgtgtatg tcgtcggcgt
     1981 attcctcaac tatctggtgc cgtcgcgcgt atttgagatt gtgttgaact tcgcgtcgct
     2041 gggaatcatc gcttcatggg cgtttatcat cgtgtgccag atgcgcctgc gtaaagcaat
     2101 taaacaaggc aaagcagcgg atgtcagttt taaactgcct ggcgcgccct tcacttcctg
     2161 gctgacatta ctgtttttac tgagtgtcct ggtgctgatg gcgttcgatt acccgaacgg
     2221 gacttatact atcgcggcgc tgccgattat cggtattctg ctggttatag gctggtttgg
     2281 tgtgcgcaaa cgcgttgctg aaattcacag cactgcgcca gtcgtcgagg aagatgaaga
     2341 aaaacaggaa attgtgttta agcctgaaac ggcgagctaa ttgcaaaatt gcccggctac
     2401 gtgccgggca aatttagatt actgacgaag gtgtgccgca tgcggcgtga taaaagtcag
     2461 gcaagtctct ccgttttcac cctcgccaca tctattacag cgcaatacga atcacatcgt
     2521 ccggctgggt agcttcctgc tgtttagtgg atttttgttt cacactgaca tacagcgttt
     2581 tgccatcggc agacagcgcc aggctgtttg gatgagtcgg cgtatcgaac gttttcacca
     2641 ctttatagct tttcgcgtca atcacactga ctttacctgc ctgacgatgc gttacgtagg
     2701 cttcattacg cgctgggtta aacagcacag ccagtgattc tggtgccgca accttcgcca
     2761 gaatattgcc attacgggta tcgaccacta acacttctgc ggctttagaa tcggtgataa
     2821 atgcacgctg cctggcggta tcaaggctaa tgttgataaa gaagtgctct ttgccgtcat
     2881 ccagcagctt tttacggctg aggattttat tgtcggcggt gtcgatggta atcaattcgc
     2941 cgtcagcgtt agtggtgtaa agacgtttgc ctttgctatc cagcgccaga ccggtactca
     3001 ttttaccggt gttctggatg gcggttttca gtttgatatt ctcgccatca acgacccaaa
     3061 tcacgctctc tttaccaata ccactgatgt aaacggtgtt cgtggcatca tcagctacca
     3121 gctcacgcgg ttgcagcggg cgcacctctt ccgtgcgctt acgatcatcc agcaccagac
     3181 ggcctttcac ctcgcccgtt ttggcatcta tcgccgtgac cgcgctgttt acggtgttac
     3241 caaaccacaa cgtctgagtc gtgttattga tggtggcacc aaacggcttg agatcgttat
     3301 ggatcgcctg cgtcacttcc agagtgaccg gatcaagacg ataaaccacg ccacctttat
     3361 ccagtttgcg gctttgcgaa gtggcgagcc acagtgcgtt ttcttgctgg ctataagcca
     3421 tttcgtaggc acctttacct accgctttac gcagcatttc ttctgcggcc tgcgtactga
     3481 atgatgaagc aacaagcaat gaacctaaca gtaatgaacc acgcaggcgc gatgaaaaca
     3541 gatgacgtaa atgcatgacg actccctttg atgaaccgat atttttgctg cttcacaatg
     3601 cttcgctaag cgtaatcatc gttacgctct tgttataaat gggaatggta atcattattt
     3661 tctcaaatgt ggagcatttc ttgccgttac gactacaatt ttctcaatta cttaacgtat
     3721 cgtgccgttt cgttttccat aaatttacaa tttcattacc ataacggcgt tttcccattc
     3781 ttaacctggc ttttttcatg aagatttttt ccgtccgaca gaccgttttg cccgcactgc
     3841 ttgtcctttc ccccgttgtt tttgccgctg atgaacagac tatgattgtc agtgccgcac
     3901 cgcaggtggt ttcagaactg gataccccag cagcagtaag cgtggtggat ggcgaggaga
     3961 tgcgcctggc aacaccgcgc attaacttgt ccgaatcact gaccggcgtg cctggtttgc
     4021 aggtacaaaa ccggcagaac tatgcgcaag atttacagct gtcgattcgc ggatttggct
     4081 cccgctccac ttacggtatt cgcggtattc gcctgtatgt ggacggtatt cccgccacca
     4141 tgcccgacgg gcaagggcaa acatccaaca tcgatttaag cagtgtgcaa aatgtggaag
     4201 tgctgcgtgg ccccttctct gccctgtatg gcaacgcgtc tggtggggta atgaatgtca
     4261 ccacccagac cggacaacag ccaccaacca ttgaagccag tagttactac ggcagttttg
     4321 gcagctggcg ctatgggctg aaagcaacgg gcgcaacggg agacggcaca cagcctggcg
     4381 atgtcgatta caccgtctca accacgcgtt ttacgaccca cggctatcgt gaccatagtg
     4441 gcgcacagaa aaatttagct aatgccaaac tgggcgtacg cattgatgaa gccagcaaat
     4501 taagtctgat tttcaatagt gtggatatca aagcagatga cccaggtggg ctaaccaaag
     4561 cagaatggaa ggctaatcca caacaagcgc ctcgtgcaga acagtacgac acgcgaaaaa
     4621 ccatcaagca aactcaggct gggttgcgct atgagcgtag cctgagcgcg caagatgata
     4681 tgagtgtgat gatgtatgcc ggagagcgag aaacgaccca gtaccagtca atcccgatgg
     4741 ccccccaact taacccgtca catgcgggcg gcgtgattac cctgcaacgc cattaccagg
     4801 ggatagacag ccgctggaca caccgtggtg aactgggcgt tccggtcacg ttcactaccg
     4861 gcctgaacta cgaaaacatg agtgaaaacc gcaagggcta caataacttc cgcctgaata
     4921 gcggcatgcc ggagtacggg caaaaaggtg agttgcgtcg cgacgaacgc aatctgatgt
     4981 ggaacatcga tccctattta cagacgcagt ggcagctgag cgaaaaactg tcgctggatg
     5041 ctggcgtgcg ctacagctcc gtgtggtttg attccaacga ccattacgtt actccgggta
     5101 acggcgatga cagcggtgat gccagttatc ataaatggct acctgccggt tcgttaaaat
     5161 atgcaatgac cgatgcctgg aatatctatc tggcagccgg gcgaggtttt gaaacgccga
     5221 cgattaatga gctgtcttat cgtgctgatg ggcaaagcgg tatgaacttt ggtttaaaac
     5281 catccaccaa cgatacaatt gagatcggca gtaaaacgcg tattggtgat gggctgctta
     5341 gtctcgcatt gtttcagacc gacactgatg atgaaattgt tgtcgatagc agtagcggtg
     5401 ggcgtacgac ttacaaaaat gccggaaaga cccgtcgtca aggcgctgaa ctggcatggg
     5461 atcaacgttt cgcaggagat tttcgcgtaa acgcgtcctg gacctggctt gatgcgacct
     5521 atcgcagcaa tgtttgcaat gaacaggatt gtaacggtaa tcggatgcca gggatcgccc
     5581 gtaatatggg ctttgcgtcg ataggttatg taccggaaga tggttggtat gcaggcacgg
     5641 aagcgcgtta tatgggcgat attatggcag atgatgaaaa tacggcaaaa gcgccgtctt
     5701 atactctcgt cggcttattc accgggtata aatacaatta ccacaattta actgtggatt
     5761 tatttggtcg tgtcgataat ttattcgata aagaatacgt tggttctgtc attgtcaatg
     5821 agtcaaacgg gcgatattac gaaccttcgc ccggacgaaa ttatggtgtc ggcatgaata
     5881 ttgcgtggag atttgagtaa ttaccttgat gcccggtatt tgccgggcat ttactttaac
     5941 gattgtattg ctggtataaa atagcaatat tttgttgcat aatttcagca agacaatgtc
     6001 tgcatccctc tttgtctttt gcttttaacg cagcgagtaa cagatgatag ttttctatat
     6061 gctcccgtaa ttccgctgga ttaatcgctt catagagata atgtaaacca ggccccatcc
     6121 tgacccacag ttgctcaatc atctcacaca ggatgggcat atttgagcga tgataaatcg
     6181 ctaagcgaaa tagcctgttt acattaatga tttgttccat gtcgcccttt tcttgcgcct
     6241 gctgtaattt ctcgagcaat tcctggagtt ccgccaggtc ttgcggggtg aggttttcaa
     6301 cagccagagc aactgccatt aattccagtt cgtagcggat ccgattgatt tcgtctaatt
     6361 gacgtttcct cacttccgga actgtaaatg cttgtgcagg tgcgacagaa agcgcattca
     6421 ccgaaaccag acgtaataat gcttcacgca caggtgtaat actcatacct aattgctccg
     6481 ccagattttt agtaatcagg cgtgcgccag gttttaatgc gccaatacta agctgatgtt
     6541 tcaggtcgtt ctcaacctgc agagtcaaac tgacatgttt catttttccc attccaggca
     6601 ttttaattcc ttcgcgtgtt ttctgtccaa ccctgcctgc acggcagaag gagttcacct
     6661 ggtgtattaa agtgaataat cataaaccac ccacctttgt tgttattagc agcagaacaa
     6721 tgacgtggtt agttaattaa agctgaaaat aagctttttg cctggatttt tatggcggct
     6781 tacgccgccg ttgaaacttt aatcatcacc cgcgacgcgg atcaccactt tgccgaagtt
     6841 tttacccttc agcaggccga taaacgtctg tggcgcattc tctaaaccat cagtaatatc
     6901 ttcgtggtag tggattttat cctctttcac ccattgcccc atctccttct gaaactcatg
     6961 gatgcggtga ccataatcct gagcgataat aaacccttgc aagcgaatac gttttttcag
     7021 caccgttgcc atcaacaggg gtaaacgatc cggaccgggt ggtagctctg tagcgttata
     7081 gctgctcact aagccacaaa cgggaatgcg cgcagatgta ttaagtaatg gtagcactgc
     7141 atcaaatacc ttaccgccca cgttttcata atagatatca ataccttttg ggcacgcttt
     7201 caccagttgt tcggcaaaat catccgcgtg gtgatcaaga caaacatcga agcccagcac
     7261 ctcgatagca tggcggcatt tttccgcgcc accggcaacc cccaccaccc tgcaaccttt
     7321 aagtttgccg atttgcccca ccgttgctcc cacaggacct gtcgccgcag ctaccaccaa
     7381 cgtttcgccc tctttaggct gaccgatatc cagcagcccc atataagcgg taaagcctgg
     7441 catccccagc acacccagcg accacgatgg attttgcgga tgatcgccaa gtttcaccag
     7501 atcatcacca ctggatatgt catagtcctg ccatccactg tagcccagca cccagtcgcc
     7561 aggctgataa tcaggatgat tcgactccac aacacggctc accgtgccgc cgaccatcac
     7621 gccgccaata tcaacaggcg gtgaataaga tggctcatcg ctcatacgtc cacgcatata
     7681 cgggtccagg gacaaataaa ctgtgcgcag taacacctgt ccttcacccg gtgtggcgac
     7741 atcatcttct tcaagacgga aattctccgg aaccggtgcg ccatgtggac gcgaggccag
     7801 aacccaacgt cgattacgct gcttttgttg cctcataatg ctctcctgtg cttttggcat
     7861 gaatccatca atagtagcag gctcacggga ttggttttgt ttgcagccgt cacatcccct
     7921 ctgcaacacg caataatttc atcgaggaat ccgttgcttc gggctgtttt cgatagtatt
     7981 gataacatca tcatcattat tttatcgagg ccagacatgt ccatccgttt tgcccgcaaa
     8041 gccgattgtg ctgccattgc ggaaatttat aaccacgccg tgttgtatac ggcggctatc
     8101 tggaatgacc aaacggttga tgcagataac cgcattgcct ggtttgaagc gcggaccata
     8161 gcaggttatc cggtgctggt gagtgaagaa gatggcgtag tgacgggata tgcctcgttt
     8221 ggcgactggc gtagtttcga tggttttcgc cataccgtgg aacattcggt ttatgtccat
     8281 cccgatcatc agggcaaagg tctggggcgt aaattgttaa gccgattgat tgatgaagcg
     8341 cgggattgcg ggaagcatgt catggtcgcc gggatcgaat cgcaaaatca ggcctcgctg
     8401 catctgcacc agtcgctggg atttgtcgtc accgcacaaa tgccgcaggt gggcactaaa
     8461 tttgggcgct ggctggatct gacatttatg cagttgcaac tcgacgaacg cactgaaccg
     8521 gacgcgattg gatgaatcag tcgctcaccc ttgctttttt gattgccgcc ggaattggtc
     8581 tggtagtcca gaacacgctg atggtacgca tcacccagac ctcctctacc attctcatcg
     8641 ccatgttgct gaactcactg gtggggattg tgctctttgt ttctatttta tggtttaaac
     8701 aaggcatggc ggggtttggc gaactggtgt ccagcgtgcg ctggtggaca cttatacccg
     8761 gcttgctggg atcgtttttt gtcttcgcca gtatcagcgg atatcagaat gtgggagcgg
     8821 cgacaactat tgcagtactg gtcgccagcc agctaattgg cggtctgatg ctggatatat
     8881 tccgcagtca tggcgtacca ctacgggcgt tgttcggccc aatttgtggc gcaattttac
     8941 ttgtggttgg cgcctggttg gtagccagac gctcatttta atcagaggat ggtgccgcct
     9001 ttggttaact gttcgtggcg cgcttccata tcttctttat gcttgcgacc gtgatgagca
     9061 atagcggtgc gcaaacgctg ctgctgggtg tagcgatctt cccgacttaa ctccgcgtca
     9121 tcgctcaggg cgattaataa ttcgttcata tgggcaatga cgctctcttc aatcgcggca
     9181 tccacgcgcg cgatgacttc atccagatgc gacatacttt ctccttaacc acttgtgaaa
     9241 tttatcgatt catccaaatg atataagtct gcgtggtgcc gtcaagccag tgagtaagat
     9301 ttaaaacagt gaaaacaaca gcgccaccgg gaaactcatc ggccatgttg cccccaccag
     9361 aaaggcgctt aagaaacgga tgcgtttctt atcgtgagaa agaaaccagg tgataagaaa
     9421 tgcgattgct gccataaccg cataaaaaac aagcatatgt tgatagagag acatgtacgg
     9481 gcgtccagcc agatgaataa aacgcgcgca aatatgcgtc attttttgac gtgcatcaag
     9541 ttttttcacc agtttgagaa aaaatcatca aacagcaact tgcccttatc catcttcaga
     9601 atgagtatta ttgcggattt tcttaacccg tactatacac ataggggcaa tgataaaagg
     9661 tggcaaaaat gaatgtttcc agtagaactg tagtactgat aaatgtcttt gctgctgttg
     9721 gtttgtttac tcttatctct atgagatttg gctggtttat ttgatgtagt gattttgcaa
     9781 gcagcacgta ctgtcagaga aatcaactct ctgacagtac gcaagatctt ctcatactct
     9841 ccaggtttac aacattatcc ttaatattta cttctttttg ctggcatcta cattgaaatg
     9901 agtagaaaac atccccctac tgtcgtctat acaaacagct tactgattca aacggaatct
     9961 tgttttcaca tttttctatc atgaatactt tatacgcgcg ggagtctata aatgctttat
    10021 actttcaagg aataacgtaa atataaaaag atggcgtaaa gtatatgcat ggcaggatgt
    10081 ttgcaacctc cttgatgacg ctgccccatt gaaattgcaa accccgccct ggttatcttg
    10141 cccatttatc atgcaaaaca gataattata ggcctctgaa tacctcagaa agattgaatt
    10201 ttttatagtc cctctcttga atatttccat ctgatatcaa tcgattcatc tcttc
//
LOCUS       DAZGOB010000154        10177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGOB010000154 DAZGOB010000000
VERSION     DAZGOB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.154"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            146..334
                     /locus_tag="ACHLIF_004336"
     CDS             146..334
                     /locus_tag="ACHLIF_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608231.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTIAVTSGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            331..1158
                     /locus_tag="ACHLIF_004337"
     CDS             331..1158
                     /locus_tag="ACHLIF_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO9608232.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITATDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGVQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKALAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGANTPFIQIK"
     gene            1199..1570
                     /locus_tag="ACHLIF_004338"
     CDS             1199..1570
                     /locus_tag="ACHLIF_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO9608233.1"
                     /translation="MNNQNTQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVINCAIESLYATLPYEEVLDERTGKTDIMATINIGELICQDEDLSGELW
                     LAGMLISAEIISIEPATNIRL"
     gene            1602..1706
                     /locus_tag="ACHLIF_004339"
                     /pseudo
     CDS             1602..1706
                     /locus_tag="ACHLIF_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            1762..2016
                     /gene="xisR"
                     /locus_tag="ACHLIF_004340"
     CDS             1762..2016
                     /gene="xisR"
                     /locus_tag="ACHLIF_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO9608234.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            2050..3336
                     /locus_tag="ACHLIF_004341"
     CDS             2050..3336
                     /locus_tag="ACHLIF_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9608235.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEIGSN
                     AFSRYQSVVRNMLPRIGEKRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPIKGRSVV
                     TVNYYMTTIAGMFQFAADNGYIVSNPFNGLTPLKRSRTEPDPLTRDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAVDALKSQAEMTRLGPQYQIDVKLREFGRTARHECTFVFNPQLVKKCQQVGH
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALSAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKQLTESVPYMPHRARL"
     gene            3320..4117
                     /locus_tag="ACHLIF_004342"
     CDS             3320..4117
                     /locus_tag="ACHLIF_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO9608236.1"
                     /translation="MEPDSEIISLNISCFSGSCRHINCVEGNTTLYALPKPEVVLRWR
                     EQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPR
                     ELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHA
                     ACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQ
                     TTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            4170..4565
                     /gene="yecN"
                     /locus_tag="ACHLIF_004343"
     CDS             4170..4565
                     /gene="yecN"
                     /locus_tag="ACHLIF_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO9608237.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            4606..5349
                     /gene="cmoA"
                     /locus_tag="ACHLIF_004344"
     CDS             4606..5349
                     /gene="cmoA"
                     /locus_tag="ACHLIF_004344"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO9608238.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            5346..6317
                     /gene="cmoB"
                     /locus_tag="ACHLIF_004345"
     CDS             5346..6317
                     /gene="cmoB"
                     /locus_tag="ACHLIF_004345"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO9608239.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(6482..8911)
                     /gene="torZ"
                     /locus_tag="ACHLIF_004346"
     CDS             complement(6482..8911)
                     /gene="torZ"
                     /locus_tag="ACHLIF_004346"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO9608240.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIRVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(8936..10036)
                     /gene="torY"
                     /locus_tag="ACHLIF_004347"
     CDS             complement(8936..10036)
                     /gene="torY"
                     /locus_tag="ACHLIF_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO9608241.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2579 a         2556 c         2400 g         2642 t
ORIGIN      
        1 tactaaccat ttatatgaaa aacaaccgtg aattaagcag agtaaaactg cttttaatcc
       61 ttgccacagt actgacaata acagaaatca ttattctctt tattgcgctg tctgtcagtt
      121 aaaaatatcg ggataccaca taccaatgag actgtatttc acaatagtaa ttttactggc
      181 aattatcgca tgcatttacg gattactcgt tccgttcctt atatccatga aggatacgat
      241 agcagttact tctggctttg cactggcgtt tctgaccccg ccctgcattt atgccattta
      301 caagggtctt tctttcacta aggataaaaa atgaaaaaaa ttatttttgc tttagccatt
      361 gttctgccga ccattggcct tgtcggctgc gatcgcgttg aacccggtaa tgttggcatc
      421 aaagtaaata aactgggcga cgataaaggc gtcggtgagg tggtcggtgt tggtcgctac
      481 tggactggct ggaatactga agtttacatc ttcccaacct tcaaacaaat gaagacctac
      541 gatgagccgt tcagcttcca gatgagtgac ggcacaacca tcggctatca catcggtgtt
      601 gcctacaaag ttgatccatc caaagttacc acggtgtttc agacctaccg caaaggcgtg
      661 gatgacatta ccgccactga cctgcgccag aagatcgccg acgcactcaa tcgactggcc
      721 agcaaaatga ccaccgacaa atttatcgac ggcggcaagt ctgaactact ggatgccgca
      781 cttaaagaca ttcaggaaga gatgaccccc atcggcgttc aggtaatgag cctctcttat
      841 gtaggtaaac cggaataccc gccaaccgtt atcgacagca ttaacgccaa agtcacggca
      901 aaccagaaaa ccctgcagcg cgaacaggaa gtcaagcaac gtgaagcgga ggccaacatg
      961 ctgcgtgcag aagctgccgg acaggctgat gccattcgaa caaaagccct ggcagaagcc
     1021 gatgccattc gtttacgcgg cgaagctctg cgccagaacc ctggcgtcat ggagctggaa
     1081 gccatcaaca agtggaacgg tacactgccg caatacatga ccagtggtgc caatacacca
     1141 tttatccaga ttaaataact tacacgcccg gcaaaccgcc gggttaatgg aaaatcagat
     1201 gaacaaccag aatactcaac cgcaaataat gaactatgac ccgaatctga cgtcgtgcgg
     1261 acgcatggca aaacaaaccg ttcgattaac tttcggacta tgggaatacc gcgaaacatt
     1321 cgaagttact gtcggcggca atctgaccgg actggatgtt atcaattgcg ctattgaaag
     1381 cctgtacgca acgctgcctt atgaagaagt cctggatgag cgcacaggga aaacggatat
     1441 catggccacc attaatattg gcgaactgat atgccaggat gaagacctgt ccggggaact
     1501 ctggcttgcc gggatgctta tctcagcaga aattatcagc attgaacccg ctacaaacat
     1561 acggctctga agttctcact attcagagag caggagaaaa aatgttcgct ctgattaatc
     1621 agggacaact gtataccgac agtgccggtt acccggtaaa aattgttcgc tgcataaaca
     1681 acaccgtgtt gtacagaaga tgtgaagcga atgtttgaag tgtggtgcca ctgccgtgga
     1741 tgaaagtttt atgaggttgg catgcagaca atcatctatc agataacccc cagcaaatgg
     1801 tgtacggaga gagtcctcat tgcatcaaca gggctaaagc ctggcaccat cgagcgagcc
     1861 agaagaaagt catggatgca gggaaaagaa taccgccatt acgctgtaga aggtgatccg
     1921 gggcattaca gtgaatgcct gtacaacatc gaagaaatta tgcgatggat cgaaaaccag
     1981 aaacaaccag gtgccaaaaa tgcaagttcc ggttaacctg ttaatgctcc tggacgtctg
     2041 ggaggtttta tgagtaacgc atcatacccg acaggcgttg aaaaccatgg aggatcactc
     2101 cgtatatggt ttcactataa tggcaaacgt gtcagagaaa acctcggtgt tcctgacacc
     2161 gccaaaaacc ggaagatcgc tggtgagctt cgcacttctg tttgttttgc aatcagaatg
     2221 gggagtttcg actacgccgc gcagttccct aattccccta acctgaaaca ctttggtctg
     2281 ggaaaaagag agataaccgt taaggcactt tcggaaaaat ggttggacct taagaaaatt
     2341 gagataggaa gtaatgcatt cagtcggtat caatccgtgg tgagaaacat gcttcctcgc
     2401 ataggggaaa aacgtcttgc ttcgtcggta acaaaggaag atttactgtt tatcaggaaa
     2461 gatttattga ccgggtatca taatctttct aacggaaaaa caacgccgat taaagggagg
     2521 tcagtagtta cggttaatta ctacatgacg acaattgcag gaatgtttca atttgcggct
     2581 gataacggct atatcgtgtc aaacccattt aacggcctga caccattaaa gagatccaga
     2641 acagaaccag atccgctcac acgtgacgaa tttattcgtt ttattgatgc ctgtcaccat
     2701 caacaaacaa aaaacctgtg gtccttagca gtatacacag gcattcgtca cggtgagcta
     2761 atatctctcg cctgggagga tattgattta aaagctaaaa caatgactat ccgtcgtaat
     2821 tatacaaaac tcggggagtt cactctacca aaaacagaag cgggaactga tcgtgttatt
     2881 catcttgttc aaccagctgt tgatgccctg aaaagccagg ctgaaatgac aagacttggc
     2941 cctcagtatc aaattgacgt caagcttcgg gagttcggtc gcaccgcacg ccatgaatgc
     3001 acgtttgttt ttaatccgca actggtaaaa aaatgccagc aagtcggtca ccactataaa
     3061 gcagattcca tcagagattc ctgggcatct gcattaaggc gagcaggact gcggcacaga
     3121 aaagcctatc agtctaggca tacttatgcc tgctgggcat tatcggcagg agcgaatcca
     3181 agcttcatag caaaccaaat gggccatgca aatgcacaaa tggtattcaa cgtttacgga
     3241 gcatggatga aagataacaa tatcgggcaa atagaactac tcaataagca gttgacggag
     3301 agtgtcccat acatgcccca tagagccaga ctctgaaata atatctttaa atatcagttg
     3361 ttttagtggt agttgccgtc acattaactg cgtggagggc aacaccacgc tttacgccct
     3421 gccgaaaccc gaggttgtcc tgcgctggcg tgaacagacc acagatgact tccgcttctg
     3481 ttttaagttt ccggcgacca tttcgcatca ggcagcatta cgacattgcg atgatttagt
     3541 gactgaattt ttgacccgca tgtcaccgtt ggctccgcgc attgggcaat actggctgca
     3601 actgcctgcc acattcggcc cacgggagct gcctgcgctt tggcattttc tcgattctct
     3661 ccctggcgaa tttaattatg gtgtggaagt ccgccatcca cagtttttcg ccaaagggga
     3721 agaggaacaa acgcttaatc gcggtttaca tcagcgcggc gttaatcggg tgattttaga
     3781 cagccgcccg gttcatgcag catgtccaca cagtgaagct attcgcgacg ctcaacgaaa
     3841 aaaacctaaa gttccggtac atgctgtact gacggcgaca aatccgctga tccgttttat
     3901 cggtagtgat gatatgacgc aaaaccggga attatttcag gtctggttac agaaattagc
     3961 gcagtggcat cagaccacta cgccttatct ttttttacat acgccagaca ttgcccaggc
     4021 cccggaactg gtacataccc tgtgggaaga cttacgtaaa acgcttccag agatcggagc
     4081 agttccggct attccacagc aatcttctct tttctgaatt tgccacctat catagacagg
     4141 tgccatcggc cattttaaag ggagtttgta tggtaagcgc gctatatgcc gttttaagtg
     4201 cgttgttatt aatgaagttc tcttttgatg tcgttcgctt gcgtttgcag taccgcgttg
     4261 cctatggtga cggtggtttt agcgaactgc aaagcgctat tcgcattcat ggtaacgcgg
     4321 tggaatatat tcccatcgcg attgtgctga tgctgtttat ggaaatgaat ggcgcagaaa
     4381 cctggatggt gcatatttgc ggcatcgttt tgcttgctgg tcgtctgatg cattattacg
     4441 gttttcatca ccgtctgttt cgctggcgac gttccggcat gagcgccacc tggtgtgcgc
     4501 tgttgctgat ggtgctggcg aatctttggt atatgccctg ggagttggtt ttctccctgc
     4561 gttagcgcac aatacgccac tttctttttc ccggattttt acgttatgtc tcaccgcgac
     4621 acgctatttt ctgcccctat cgccagactg ggcgactgga cctttgatga acgggtagct
     4681 gaagtcttcc cggatatgat ccagcgttcc gttcccggct attccaatat tatttccatg
     4741 attggtatgt tagccgagcg cttcgttcaa cctggtacgc aggtttacga tctgggttgt
     4801 tctctgggcg cggcgacgct ctcggtgcgt cgcaacattc atcatgataa ttgcaaaatt
     4861 attgccatcg acaactcccc ggcgatgatt gaacgctgcc gtcgtcatat tgacgcctat
     4921 aaagccccta cgccagtaga cgtcattgaa ggtgatattc gcgatatcgc cattgaaaac
     4981 gcatcgatgg tggtgctgaa ttttaccctg caattcctgg aaccttccga gcgccaggca
     5041 ttactggata aaatttatca aggactgaac ccgggcggcg cgctggtgct ttcggaaaaa
     5101 ttcagtttcg aagatgccaa agttggtgaa ctgttgttca acatgcacca cgactttaaa
     5161 cgtgccaacg gttacagcga actggagatc agccagaagc gcagcatgct ggaaaacgtg
     5221 atgctgaccg attccgtgga aacccataaa gcacgcctgc ataaagccgg ttttgagcat
     5281 agcgagctgt ggttccagtg ctttaacttt ggttcactgg tggcattaaa agcagaggac
     5341 gctgcatgat cgactttggt aacttttatt ctctgattgc caaaaatcat ctttcacact
     5401 ggctcgaaac gctgcccgcg cagattgcta actggcagcg cgagcagcag cacgggctgt
     5461 ttaagcagtg gtccaacgcg gtggaatttc tgcctgaaat taaaccgtat cgtctggatt
     5521 tattgcatag cgtaaccgcc gaaagcgaag agccactgag caccgggcaa attaaacgca
     5581 ttgaaacgct tatgcggaac ctgatgccat ggcgcaaagg gccgttctca ctgtatggcg
     5641 tcaacatcga taccgaatgg cgttccgact ggaaatggga tcgtgttctg ccccatcttt
     5701 ctgatttaac cggacgcacc attctggatg tcggctgcgg cagcggttat cacatgtggc
     5761 gcatgattgg cgcaggcgcg cacctcgcgg tggggatcga ccctacgcaa ctgtttctgt
     5821 gccagtttga agcggtgcgt aaactactgg gtaacgatca gcgcgcacat ttgttaccgt
     5881 taggtattga acagcttccg gcactgaaag cctttgatac cgtcttttcg atgggcgtgc
     5941 tttaccatcg ccgttcgccg ctggagcatc tctggcagtt gaaagatcaa ctggtgaatg
     6001 agggtgaact ggtgctggaa acgctggtta ttgatggcga tgagaacacg gtgctggtac
     6061 caggcgatcg ttacgcgcaa atgcgtaatg tctatttcat cccttccgcg ctggcgctga
     6121 aaaactggct gaagaagtgt ggttttgtag atatccgcgt tgtggatgtg tgcgttacca
     6181 ctactgaaga gcagcgacgc actgaatgga tggttaccga atctctgtcc gatttcctcg
     6241 acccacatga tccgagtaag acagttgaag gttatcctgc gcctaaacgc gcggtgctga
     6301 ttgcgcgcaa gccgtaaagg tctggcatta ctgccggatg cggcttgaat gccttatccg
     6361 gcctacaaac ccttgctaat tcaatctatt gcagggacta tgtaggcctg ataagcatag
     6421 cgcatcaggc agttttacgt ttgcataacc tcagcgcccg tttccgggcg ctattcactt
     6481 cttacgcctg aacagcaggc ttatcaaatg ccgttaactt cggtgcgtta cccgtatatt
     6541 tttcgatata caccagcgca gagttaccgg cacaggcatt tgccagctgc gagctgggga
     6601 tatccgccgt taacacgttc gcactgccgt ttttacacaa gccgttttcc agatctggcc
     6661 atgcaccttc atgcacgcat accacgcctt ttttgatccc gtcagtcacc accgcgcctg
     6721 tcaaaatctg accacgtttg ttccagacac gcaccagatc gccattcgca ataccaaatc
     6781 gagcagcatc ttcggtgtga atagtgatcg gttcacgatc ggcaaccgca tattttttac
     6841 gcagttccgc atagttaagc tgactatgta aacggtgtgc cggatgtgcg gtcagaagct
     6901 gcaactgctt ctcgtcggcg gtacccttcc actcatcagg cgcaagccag gttggatgtg
     6961 ccgggcaatc cttatagcca aatttttcca gcgttttgga gtaaatctca attttgccgc
     7021 ttggcgtacc cagcgcattt ttcaccggat cggcgcggaa atcagcataa cgaacgtact
     7081 gttcgttctt ctcgctgcgg cgcatttcga tcagtttatt ttgctgccag aaggcattaa
     7141 acatcggcat agtcacgcgt tgcgcacgag cacctttctg agcggcatca tagaaaaatt
     7201 tcagccacgc catttcatct ttaccttcgg tatagatctc ttttccgcca ggtttcagta
     7261 attcagcaag atcggcaaat acgtcaaaat cgttacgcgc ttcaaattgc ggagcaacag
     7321 cctgcttcat cggcacaata tgctggttgc tgtaatcacc ggtcatcgtc aagtcattgc
     7381 gctcaaacga tgtggtgatc ggtaatacga tatctgcatg tttagcagct gcggtccagt
     7441 agcattcaga aacgacgatc atctccggtt tctgccatgc tttgatcaga cgattagtat
     7501 cctggtgatg ggtaaagtta ccgccacccg cccaccagat cattttgata ttcggatacg
     7561 tttgttcttt accgttatgc tgatatttgc caccaggatt ttccagcgca tcgacgatac
     7621 gggcaacacg aatcgccgtc attccgccat catcagcggc ctcgctggcc tgtccggcaa
     7681 tcgccgcaga catttccggc agcacgccgc caacacgcgt cggattgcca ccgttggagt
     7741 aatgataaga gaaaccaaaa ccgccgcccg gtgtaccaat ctgccctaac atcgccgcca
     7801 gcgtcaccag catccagtgt ttctgttcac catattgctg gcgctgaatt ccccagccag
     7861 ccatcagcat tgtgcgattc gccgccatca gttcagcgag tttgactatc tgcgcttccg
     7921 gtacgccagt aatttccgcc gcccaggccg cgctcttcgg agtattatcg ctcttacctg
     7981 tcagatactc ttcaaactgc ggatacccgg tggtgtattt ttcaaggaac actttatcgt
     8041 gtttaccttg tgtcatcagg gtatgcgcaa tccctaacat cagtgccacg tcggtgccca
     8101 tattcggcgc gatccaggtg gcattatcac caaagaactc gatggtttcg gagcggatag
     8161 gatcaatggc aatcactggt ttgccagatt ttttcagctg atggaagtat tccagccctt
     8221 gctcatcggt actgctccag gcaattttta aggtattcag cgggttcatc ccccacagca
     8281 ccacagcctg gctgttttcc agaatcagcg gccaggaggt ctgctgttca tacacctcta
     8341 cagaaccgac cacatgcggc atgatcacct gtgctgcccc ggttgaataa tcgccgctat
     8401 gcccggaata accgcccgcc aggttcatat aacgttgcag taaggtttgc gctttatgca
     8461 acacgccaga agagcgccag ccgtatgatc cggcaaaaat agcagatggt ccgttagctt
     8521 tgcgaatgcg atcgtgttgc tcatgaatca gttttaatgc ttgctcccag ctcacctgta
     8581 cataagtatc ttcgccacga cctttcgccg gttgcagtgg attatcgaga tagctttttc
     8641 ttaccatcgg atgctgaata cgtgccgtgg tgtgtacctg atccgccgcc gtagactgta
     8701 aggagttcgg tatggttttc gccagcgcgc ctgttgaaga aacaatcttg ccgtccttca
     8761 cttctacgtt catcgctccc caacgtcccg cggtgaggat tttaccgccc ttctcttctg
     8821 cccatgcggg gagcggtgct gccgatgtca ccaccagcgc tccagcggca ataccgctgt
     8881 gtttaataaa ttcacgtctt gttaatgtca taacttcctc cctgatcaac gaggatcact
     8941 gtttctcggt aatatctttg gcgttgtact ggaaataccg cgttaaaatg tccagttcgt
     9001 tttcgctcat gctggttcgc gcacccattc ctttggcaat ggacggccat gcattgacgg
     9061 tgtaatggtc agcggcaata ggcgcatggc aaccagcgca ataggtatcg tcaagttttt
     9121 cagcgtattg ccatagcggt ttacggtccg ctaatgcggg atcggtaagc gcaccctgta
     9181 aagacgcctg acgccattga ttgccgtatt cgtcagcctg ccattccccg tttaccgtga
     9241 gcgccttgat accttcttca cttaatgtgg ctagcgccag ccgttgacct gccgccaggt
     9301 agagcgtgtt ttcactgccc tgcatttgat aaccctgcaa cagaacgatc ggctgtttgc
     9361 cactggcatc aacgacggtg agatcggtac caggattcac ggtagccagc tcgcctatgc
     9421 gagaagtttt gaaaggataa atatgtgcgc cattagtcac tgaagtagcg gcctgacttt
     9481 ccagctcatg cgccgcgttg tcatccattt ttatttctgg cggaaaatgg gcaatgcctt
     9541 tatgacaatc gatacaggtt tcgccgccct tttgtgcttt gttatgcatt ttctgcgcag
     9601 attcactttg cgaggcaata tccatggcat caaaagaatg gcaactacgg cacgttgcag
     9661 agtcagttgc ttttaattct ttccatactg tttcggccat ttcctggcga tgagtttcga
     9721 acttatcgtc actgtctatt ttgccgctaa caaattcatg ataaatatct ttagatgctt
     9781 ttaatttagc aaataaataa tccatccctg actttggaat atggcaatct gcacattccg
     9841 cacgtatccc tttctggttc gaaaagtgga cagttccctg atattcctca aaaggtttac
     9901 tcatcgagtg gcaggaaagg caaaatgctg tatccgacgt tttatgtaag gctttttgcg
     9961 ccagcaataa cccgccgcca ccaaccacaa ccgctatcag caaaaacaat aacccaatgc
    10021 gttttttccc tcgcatagat tcctctaaat aaattccaga acaccgaaat cattgtataa
    10081 ttaaatacac aacgaattaa ccgcttgcat aactcggcac aacactgcct gaaataatcg
    10141 ataaagaata tgatttatta caatgtaatc attaatt
//
LOCUS       DAZGOB010000155        10121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGOB010000155 DAZGOB010000000
VERSION     DAZGOB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.155"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            203..1576
                     /gene="aroP"
                     /locus_tag="ACHLIF_004348"
     CDS             203..1576
                     /gene="aroP"
                     /locus_tag="ACHLIF_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO9608242.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAMIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1619..2473)
                     /gene="ampE"
                     /locus_tag="ACHLIF_004349"
     CDS             complement(1619..2473)
                     /gene="ampE"
                     /locus_tag="ACHLIF_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO9608243.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2470..3021)
                     /gene="ampD"
                     /locus_tag="ACHLIF_004350"
     CDS             complement(2470..3021)
                     /gene="ampD"
                     /locus_tag="ACHLIF_004350"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO9608244.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3109..4002
                     /gene="nadC"
                     /locus_tag="ACHLIF_004351"
     CDS             3109..4002
                     /gene="nadC"
                     /locus_tag="ACHLIF_004351"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO9608245.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4205..4645
                     /gene="ppdD"
                     /locus_tag="ACHLIF_004352"
     CDS             4205..4645
                     /gene="ppdD"
                     /locus_tag="ACHLIF_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO9608246.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4655..6040
                     /gene="gspE"
                     /locus_tag="ACHLIF_004353"
     CDS             4655..6040
                     /gene="gspE"
                     /locus_tag="ACHLIF_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO9608247.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6030..7232
                     /gene="hofC"
                     /locus_tag="ACHLIF_004354"
     CDS             6030..7232
                     /gene="hofC"
                     /locus_tag="ACHLIF_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO9608248.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7267..8310)
                     /gene="guaC"
                     /locus_tag="ACHLIF_004355"
     CDS             complement(7267..8310)
                     /gene="guaC"
                     /locus_tag="ACHLIF_004355"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO9608249.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8466..8510)
                     /locus_tag="ACHLIF_004356"
     CDS             complement(8466..8510)
                     /locus_tag="ACHLIF_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608250.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8535..9155
                     /gene="coaE"
                     /locus_tag="ACHLIF_004357"
     CDS             8535..9155
                     /gene="coaE"
                     /locus_tag="ACHLIF_004357"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO9608251.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9155..9898
                     /gene="zapD"
                     /locus_tag="ACHLIF_004358"
     CDS             9155..9898
                     /gene="zapD"
                     /locus_tag="ACHLIF_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO9608252.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9908..10105
                     /gene="yacG"
                     /locus_tag="ACHLIF_004359"
     CDS             9908..10105
                     /gene="yacG"
                     /locus_tag="ACHLIF_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO9608253.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT         2484 a         2664 c         2594 g         2379 t
ORIGIN      
        1 gtttcttttt aagcaactca tcttcaacca tgcataaagc gggtgcattc gctgcctcat
       61 accattattc ttgatctgac ggaagtcttt ttgtaacaat tcaaacttct ttgatgtaaa
      121 caaattaata caacaaacgg aattgcaaac ttacacacgc atcactgcgt agatcaaaaa
      181 aacaaccacc gcacgaggtt tcatgatgga aggtcaacag cacggcgagc agctaaagcg
      241 cggccttaaa aaccgccata ttcagcttat cgcgctgggt ggcgcgatag ggaccgggtt
      301 attcctgggt agcgcctccg taatacagtc cgcagggcca gggattatcc tgggttacgc
      361 cattgctggt tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg tggtcgaaga
      421 acctgtcgca ggctccttta gccactttgc ttataaatac tggggcagtt ttgccggttt
      481 cgcctctggc tggaactact gggtactgta cgttttagtt gccatggctg agctgactgc
      541 cgtgggtaaa tacattcagt tctggtatcc ggaaatcccc acctgggttt ctgccgccgt
      601 attctttgtg gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg gcgagatgga
      661 gttctggttt gccattatca aagttatcgc ggtggtagcg atgatcatct tcggcggctg
      721 gctgctattc agtggcaacg gcggcccgca ggcgaccgtt agcaacctgt gggatcaggg
      781 tggtttcctg ccgcacggct tcaccgggct ggtgatgatg atggcgatga tcatgttctc
      841 gttcggtggt ctggaactgg tggggatcac cgcagcagaa gctgataacc cggagcaaag
      901 tataccgaaa gcaactaacc aggttatcta ccgcatcctg attttctata ttggttcgtt
      961 agccgttctg ctctcactga tgccgtggac ccgcgttacc gccgatacca gtccgtttgt
     1021 gctgatcttc cacgagttag gcgatacctt tgtggcgaat gcgctgaaca tcgtggtact
     1081 gactgcggcg ctctccgtgt acaacagctg cgtatattgc aacagccgta tgctgtttgg
     1141 tctggcacaa cagggtaatg cgccaaaagc gctggcgtct gtcgataaac gtggtgtacc
     1201 agtaaatacc attctggtgt ctgcactggt aacggcgttg tgcgtactga ttaactacct
     1261 tgccccagag tccgctttcg gactgttaat ggcgctggtg gtatctgcac tggtaatcaa
     1321 ctgggcgatg attagcctgg cgcatatgaa attccgtcgc gccaagcagg aacaaggcgt
     1381 ggtaactcgc ttccctgctc tgctttatcc gctgggtaac tggatctgcc tgctgtttat
     1441 ggcggcggta ctggtgatta tgctgatgac cccaggaatg gcgatttcgg tatacctgat
     1501 cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa ccgccaaagc
     1561 cgtaaaagcg cattaatctc tctacgccct cacccgtaca gggtgagggc aataatcttt
     1621 acaccaacgc cccgtaaatc gtcagtagtg caatcaccac cacgaccacg aacgaggttt
     1681 tcttcgccat tgaaaccgct gccctcggcg tctccacctt atcgacatgc ggttcacgcg
     1741 ccagagagaa ctgcgccaga cgcgttaaca cctgatactg cgaagtatgg aaatcaccca
     1801 gcgaagcaaa ccaggccggt aacgctttct caccatgacc gatcaaggca tataccacac
     1861 ccgcaagacg aaccggcacc caatccagta catgaagcac ggcatcaatg ccggactgta
     1921 aacgatgatg cggcgtctga tatcgcgcca gccagtattg ccatgcacgc aaaaacgcat
     1981 accccatcag cgtaacgggt ccccaggttc cccccacaat cagccagaac agcggtgcaa
     2041 gataaaaacg aaagttaatc cacagcaatg cattttgcag ctcacgcaaa tactcacgtt
     2101 cgtcgcagcc tgccgggacg ccgtgaatca tggtgagttc gccagccatc gtggcacggg
     2161 catggctatc attacgtgaa gcagctgtca gataagcatg ataatgaaga cgaactttac
     2221 ctgcgccaat acacagcaaa ccaatcagca gccacaccag tagcgtggga acgttgaaca
     2281 atactccctg caatgcgcgt aacagtaaaa aagtcacgcc catcgcaata atggtcatgc
     2341 ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa gaacgcttca agacgatgat
     2401 caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc agtaaggttg
     2461 taaatagcgt catgttgtct ccttgctgac cagcacacga aaccgtgccc aatcaaatgc
     2521 aggaccggga tcggttttcc gatccggcgc aatatcacaa tggcccgtca tgtttttagc
     2581 gatatccgga tagcaatcaa tcagtgcccg cgtaaccgcc gcaagctgtt gatactgcgc
     2641 atcggtatac gccagcgtat cggtgccttc aagctcaatc ccaatagaaa aatcattgca
     2701 gcgttcgcgc ccctgatact gagagactcc cgcatgccat gcacgtttat cgaaaggaac
     2761 atactggact atttcaccat cacggcgaat caaacagtga gcggagacgc gcaaatgggc
     2821 gatctcagca aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata atgcgtcgat
     2881 ccacggaccg ccaaactcgc ctggcggcag gctaatattg tgcaccacca gcagggtggg
     2941 tgtttcgtca tccgggcggc aatcgtaatg tggtgaggga acgcggcgcg cgccaaccag
     3001 ccacccctgt tctaacaaca tgcaggatct ccttatatgt ggtgctaata cccggttcag
     3061 agtagcatgt ttctacctta tgattcgtta gctatctgga gttttaacat gccgcctcgc
     3121 cgctataacc ctgacacccg acgtgacgag ctgctggaac gcattaatct cgatatcccc
     3181 ggcgcggtag cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc caacaatgat
     3241 attacggcaa aacttttacc ggaaaattct cgctctcatg ccacggtgat cacccgcgag
     3301 aatggcgtct tttgcggcaa acgctgggtt gaagaggtgt ttattcaact ggcaggcgac
     3361 gatgtcacca taatctggca tgtggatgac ggcgatgtca tcaatgccaa tcaatccttg
     3421 ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc gcttaatttt
     3481 gtgcaaaccc tttcaggagt tgccagtaag gtacgccact atgtcgaatt gctggaaggc
     3541 accaacacgc agttgttgga tacgcgcaaa accttacccg gcctgcgttc agctctgaaa
     3601 tacgcggtac tttgcggcgg cggagcgaat caccgtctgg ggctttctga tgccttcctg
     3661 atcaaagaaa accatattat tgcctccggc tcagtgcgcc aggcggtcga aaaagcgtcc
     3721 tggctgcacc cggatgcgcc agtagaagtc gaagtagaga atctggaaga acttgatgaa
     3781 gccctgaaag caggagccga tatcatcatg ctggataact tcgaaacaga acagatgcgc
     3841 gaagccgtca aacgcaccaa cggcaaggcg ctactggaag tgtctggcaa cgtcactgac
     3901 aaaacactgc gtgaatttgc cgaaacgggc gtggacttta tctccgtcgg tgcgctaact
     3961 aaacacgtac aagcactcga cctttcaatg cgttttcgct aatagttgac agatttatcc
     4021 gctccatcgc gggcggataa tctccccttc cccaactttc ttcgtaacgc ctcgcaaatt
     4081 tcttgtcttt cgctgcaacg aaataaaaaa cctcggaatc tgctttcccg tttcgttttt
     4141 tgcccctcgc ctgtgctgat ggcgtttgcc aaagtagcgc caactaaatc aaggagcgaa
     4201 acagatggac aagcaacgcg gttttacact tatcgaactg atggtggtta ttggcatcat
     4261 tgccatttta agcgccattg gtattcccgc ttatcaaaac tacctgcgca aagccgcact
     4321 caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt gcgcgctgga
     4381 acatggtgga ttagatacct gcgacggtgg cagcaatggc attccctcgc ctaccaccac
     4441 ccgctatgtt tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg ggcaagaaag
     4501 tctcaatggg ctaagcgtcg tcatgacacc gggttgggat aacgcaaacg gcgtcaccgg
     4561 ctggacgcgc aactgcaata ttcaaagtga cagcgcattg catcaagcct gcgaagatgt
     4621 cttccgcttt gatgacgcca actaaggagc ggcaatgaat attccacagc tcactgccct
     4681 gtgtctgcgt tatcatggag tcttgctgga tgccagcgaa gaggtggttc atgttgcggt
     4741 agtcgatgca ccttcgcatg agctactgga cgcattgcat ttcgctacca ccaaacgtat
     4801 tgagatcacc tgctggacgc gccaacaaat ggaaggtcac gccagtcgca cacaacagac
     4861 attgcccgta gctgttcagg agaagcatca gcccaaagca gagttgctaa ctcgaacgtt
     4921 acaatctgcg ctggaacaac gcgcgtctga tattcatatc gaaccagcgg acaatgccta
     4981 ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta ccggatgttt caccggatgc
     5041 cggagtcgca ttaaccgcca gattaaaagt gctgggaaac ctggatattg cggaacatcg
     5101 cctgccgcag gacgggcaat tcactgtcga actggcagga aacgccgtct catttcgtat
     5161 tgcgacctta ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac agcaggtgag
     5221 ccaggcactg gatgtcaaca cgcttggaat gcagccgtta caactggcgg actttgctca
     5281 tgccttgcaa caaccacagg gactggtgct ggtaactggc cctacaggca gcggcaaaac
     5341 ggtcacgctt tatagtgccc tgcaaacgct gaataccgct gacattaata tttgtagcgt
     5401 cgaagatccg gttgagatcc ccatagccgg actaaaccag acgcaaatcc atccgcgtgc
     5461 cggactcacc tttcagggcg tgttgcgtgc gttattgcgc caggatcctg acgtcatcat
     5521 gatcggagag atccgcgatg gcgaaacagc agagatcgct attaaagcgg cgcaaactgg
     5581 tcacctggtg ttgtctaccc tacacactaa ttccacctgc gaaacgctgg tacgtttaca
     5641 gcaaatgggg gtcgcccgct ggatgctatc atcggcgctt acgctggtaa tagcccagcg
     5701 tctggtacgc aaactttgcc cacattgtcg ccggcagcaa ggggagccca tccacattcc
     5761 agacaatgta tggccatcgc cgctgcccca ctggcaggca cccggttgtg tacattgcta
     5821 ccacggtttt tatggtcgta cggccttatt tgaagttctg cccataacgc cggtcattcg
     5881 tcagcttatt tccgctaata ccgacgttga atcgctggaa acgcacgcac gacaggcggg
     5941 tatgcgtacg ctttttgaaa acggctgcct ggccgtggag caaggcttaa ccacctttga
     6001 agagttaatc cgcgtactgg ggatgccgca tggcgagtaa gcaactctgg cgctggcatg
     6061 gcataaccgg cgacggcaat gcgcaagatg ggatgctatg ggcagagagc cgtgctttgc
     6121 tgctcatggc actacagcaa cagatggtta ccccacttag cctgaagcga atcgccatca
     6181 attctgcgca gtggcgagga gataaaagcg cggaagtcat tcatcaactg gcgacgctac
     6241 tcaaagccgg gttaacgctt tctgaagggc tggcactgct ggcggaacag catcccagta
     6301 agcaatggca agcgttgctg caatcgctgg cgcacgatct cgaacagggc attgcttttt
     6361 ccaatgcctt attaccctgg tcagaggtat ttccgccact ctatcaggcg atgatccgca
     6421 cgggtgaact gaccggtaag ctggatgaat gctgctttga actggcgcgt cagcaaaaag
     6481 cccagcgtca gttgaccgac aaagtgaaat cagcgttacg ttatcccatc atcattttag
     6541 cgatggcaat catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta
     6601 tctataagac cttcaacacc ccactaccgg cactaacgca ggggatcatg acgctggcag
     6661 actttagtgg cgaatggagc tggctgctgg tgttgttcgg ctttctgctg gcgatagcca
     6721 ataagttgct gatgcgccga ccgacctggc ttatagtgcg gcagaaattg ctgttacgca
     6781 tcccgattat gggttcactg atgcggggac aaaaactcac gcagattttt acgattctgg
     6841 cgctgacaca aagtgcaggc attacttttt tgcagggcgt agagagcgtc agagaaacaa
     6901 tgcgctgccc gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc
     6961 atcccatctg gctggcgcta aaaaataccg gggagtttag cccgctctgt ttgcaattag
     7021 tgagaacagg agaggcatcc ggctcgctgg acctcatgtt agacaacctc gcccatcatc
     7081 atcgggataa cacaatggcg ctggcggata acctcgcagc cttactggaa ccggcgttgt
     7141 tgatcataac gggaggaatt atcggtacgc tggtggtggc gatgtatctg ccaattttcc
     7201 atttaggcga tgcgatgagt gggatgggat aacgctggcg tgttgctcca caccagcgtt
     7261 ggaagattac aggttgttga agatgcggtt ttcttgttcc tgcacacgga taaatgtggt
     7321 gcgcttggtc agttctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc
     7381 gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc agcggcagct taacggtttt
     7441 accttctgct gcgcgatatt ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga
     7501 gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct
     7561 ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc
     7621 ttttgcgaca tcgcccggcg tggtgcagcc accgtcgctg acgatcattc cgcccagacc
     7681 gtgcgccgca tcggcacatt cgattaccgc agaaagctgc ggataaccga cgcctgtttt
     7741 gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga
     7801 gaggataagc tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt
     7861 cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc
     7921 cacatcaata caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc
     7981 gaaatccgca tcagacgtac cggtagaaac catcacatgt ttcagcacat cagcggaaga
     8041 attgttgata aacgcttgcc actcttcgac agaatagtgt ttatgcacag cagtcaaaat
     8101 atcaaaagaa gccagcgcag aggccataga aaatgtacct acggtgtcca tatttgcggc
     8161 gataattggc acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag
     8221 ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa
     8281 acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag
     8341 ggtaagagcg caactccagt gacgctatca tacgcactaa tcaatgcggc gcaagactgc
     8401 gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg
     8461 tttcatcaat ttcgctttcg tgcactcatt tttaacgttt tgataatcat gatgaattcc
     8521 cgggaatata aattatgagg tatatagttg ccttaacggg aggcattggc agtggcaaga
     8581 gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg
     8641 cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg
     8701 ctaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg
     8761 ccaacccgga agagaaaaac tggcttaacg ccctgctgca tccgctgatt cagcaagaga
     8821 cgcaacacca gatccagcaa gctacttccc cctatgtact gtgggttgtg ccattgctgg
     8881 tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa
     8941 cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg cgagcatgtc gaacaaatcc
     9001 ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata
     9061 acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc
     9121 ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca
     9181 tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac
     9241 cgttaattta cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagtga
     9301 attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttgaccg
     9361 gcagcaacgt aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga
     9421 agcattaatt cagcagttaa aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg
     9481 gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg
     9541 cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca
     9601 gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac
     9661 catggtgctg gatttaattc gccagtcggc tcccttccgt aaacaaacca gcctgaatgg
     9721 tttttatcag gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc
     9781 acagctttat ccgcaaattt ccggacataa gagccgtttt gccattcgtt ttatgccgct
     9841 ggacactgaa aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg
     9901 agtgaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg
     9961 gggtgaaatc agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg
    10021 agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga
    10081 ctggagcgaa gagccaaagc agtgacattt gtctgatgcc g
//
LOCUS       DAZGOB010000156        10053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGOB010000156 DAZGOB010000000
VERSION     DAZGOB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.156"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(74..676)
                     /gene="fimB"
                     /locus_tag="ACHLIF_004360"
     CDS             complement(74..676)
                     /gene="fimB"
                     /locus_tag="ACHLIF_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO9608254.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2132..2848
                     /gene="nanC"
                     /locus_tag="ACHLIF_004361"
     CDS             2132..2848
                     /gene="nanC"
                     /locus_tag="ACHLIF_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO9608255.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVDVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2868..3974
                     /gene="nanM"
                     /locus_tag="ACHLIF_004362"
     CDS             2868..3974
                     /gene="nanM"
                     /locus_tag="ACHLIF_004362"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO9608256.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWKALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4039..5019
                     /gene="nanS"
                     /locus_tag="ACHLIF_004363"
     CDS             4039..5019
                     /gene="nanS"
                     /locus_tag="ACHLIF_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9608257.1"
                     /translation="MNAIISPDYYYILTIAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(5027..5677)
                     /locus_tag="ACHLIF_004364"
     CDS             complement(5027..5677)
                     /locus_tag="ACHLIF_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9608258.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            5814..5957
                     /locus_tag="ACHLIF_004365"
                     /pseudo
     CDS             5814..5957
                     /locus_tag="ACHLIF_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(6621..6749)
                     /locus_tag="ACHLIF_004366"
                     /pseudo
     CDS             complement(6621..6749)
                     /locus_tag="ACHLIF_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(6725..6874)
                     /locus_tag="ACHLIF_004367"
                     /pseudo
     CDS             complement(6725..6874)
                     /locus_tag="ACHLIF_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            complement(6959..7156)
                     /locus_tag="ACHLIF_004368"
     CDS             complement(6959..7156)
                     /locus_tag="ACHLIF_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9608259.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(7168..7656)
                     /locus_tag="ACHLIF_004369"
     CDS             complement(7168..7656)
                     /locus_tag="ACHLIF_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9608260.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(7653..8027)
                     /locus_tag="ACHLIF_004370"
     CDS             complement(7653..8027)
                     /locus_tag="ACHLIF_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9608261.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(8117..8485)
                     /locus_tag="ACHLIF_004371"
     CDS             complement(8117..8485)
                     /locus_tag="ACHLIF_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9608262.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(8648..8869)
                     /locus_tag="ACHLIF_004372"
     CDS             complement(8648..8869)
                     /locus_tag="ACHLIF_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9608263.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8932..9408)
                     /locus_tag="ACHLIF_004373"
     CDS             complement(8932..9408)
                     /locus_tag="ACHLIF_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO9608264.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(9424..9909)
                     /locus_tag="ACHLIF_004374"
     CDS             complement(9424..9909)
                     /locus_tag="ACHLIF_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9608265.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9964..>10053)
                     /locus_tag="ACHLIF_004375"
     CDS             complement(9964..>10053)
                     /locus_tag="ACHLIF_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HFO9608266.1"
                     /translation="TRAIHSIDTDIKLNRVLWVMAETLLESLR"
BASE COUNT         2784 a         2252 c         2392 g         2625 t
ORIGIN      
        1 aatggttaat catttttgat atatcgtaag aataatgtag tttttaacac catccctggt
       61 atctcaacta tctctataaa acagcgtgac gctgtcgtcc tctggctcta tcccagatgc
      121 cgtaaaaacg ccctgcattg ctggcggtat accagacagt atgacgaata ttgcgatgcc
      181 caagataatc ctggataagt cgcgtatcta ttcccatatt cgccaaagca aaaccacacg
      241 aatggcgtaa catgtgcgga tgaatctcca gtgacaaccc ggcattacca ccggaagtcg
      301 agataatatg gtaaaactgt tgccgagaaa gcggatttcc cttacgcgat aaaaataccc
      361 attcgctctc agcatgcggg tacgaagtac ggatactcaa ccagtttttt aaagcctgaa
      421 cttctttgtt caatagcggg tgcgttgttg aaaagccttt ttttaatcga tggatatata
      481 tacactttgc cttaagatca atatccgaaa tcctcaatcg acaaatttca ctcgcccgga
      541 aaccatgaat aaaacaaagc aaagtcagac aataattacg tgctgcatga ggcccggtat
      601 ttgctgcttt aaggagtgat tcgatttcac tatgggtcag gaagttcctt tttttgttat
      661 cagccttatt cttcatcgtt tttcccttat aattacagac gcgcactagc tgtgctgggt
      721 tagcaataat ccaacatttt tatcctgatt atgtttataa aagcgaacgt ttgcttaata
      781 actaacgtaa gtggaccagt tcttctgagt gaacttaaat ggagtagcaa cagttactta
      841 taataacgtt gccatacaaa cgctatgctc ttgcatgcta tgtaccttta tatatttatc
      901 aatcggatac gaatctaata ataaccctca tcattaatgc aactaatctt atctatatat
      961 catgtgatat gttctgtaac aagtaaaatc aacataaaaa attcagcaaa atttaatatt
     1021 cgttcaacta tccgtctcac atatgacatt attagacaac tccatttgcc acaaaatggc
     1081 tatttcatgg aacgcagtaa tttcttagta gctattatca ggctaattct taggtcccta
     1141 gcgattattc ctggctggtt cgtcagttta gttcatgaga ttgttacatt cctttttatc
     1201 ctggagatat gggggaaagc aggagatgat tttttcatct gcatccgttg gtaaatatgg
     1261 ctttggtcac tgcgacttaa accttcctca atcacagctg ttcgccgggc tattcggcaa
     1321 acaagctaag tgaaacatca tcctgaaaac accaacatca acaagcctct ccagagagac
     1381 ttcaggagtg accagctaca agccacaaac caggaaacat atttcatgat gagaattatg
     1441 ctcagcctga tggcgggtaa acacttattt tcaccacgcc cactgagtta tcttgcaaat
     1501 ctttccttgt ttatactcta aataaattac ttataaaaca gataattaaa taacaaccct
     1561 tggtttgtga aggcctccgc aagtgttcgt ttggcttcac atggcatctt cttcttagaa
     1621 aaatatcgac atattttgtg acacgaattg caaatctggt tttgttgtat ggattgcgtg
     1681 atttttgatc tggtataaca ggtataaagg tgcaccaaga tagtcaatga gacagggcat
     1741 ctcgcaatct atggcaaaca tcacttcagt tctttcacat cgggtgatga aaatgcactt
     1801 cagtctgaaa ggaatatgaa aatgagatca acagacactc tattttatga ctctgggtaa
     1861 aatggattga gtaagtgata tagcttacga acattcaaat caattaaaca tcagaagaga
     1921 ttttatactc aggtatttaa tctggctctc tgtttattta aataatgtga aaagagattt
     1981 ttcacaggag accttataca aaaaatataa aatacagcta ccggttgcca aagacactat
     2041 aagcctggca aaaaaatatt acacaacata aatgctaatt gtttatgcgg gctttgtatt
     2101 gctttctgtt tcctacaaat gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt
     2161 attattactg tgcttttcgt caccattaat ttctcaagct gcgacactgg acgtacgtgg
     2221 tggatatcgt agtggaagcc acgcctatga gactcgactc aaagtcagtg agggatggca
     2281 aaatggatgg tgggcaagta tggaaagtaa tacctggaat accattcatg ataataaaaa
     2341 ggaaaatgcc gcactcaatg atgttcaggt tgatgttaat tacgcgatta aacttgatga
     2401 tcaatggacg gtgcgcccgg gaatgttaac gcattttagc agcaacggca cacgctacgg
     2461 accctacgta aaactgtcct gggacgcgac aaaagatctt aattttggca ttcgctatcg
     2521 ttacgactgg aaagcttacc gacaacaaga cttatccggt gatatgtctc gtgataacgt
     2581 tcatcgttgg gatggatatg tcacttacca tattaatagt gatttcacct tcgcatggca
     2641 aacgacgcta tacagcaaac agaacgatta tcgctatgca aaccataaga aatgggcgac
     2701 ggaaaatgca tttgttctac aataccatat gacgccggat attacgccat acatagaata
     2761 tgactacctt gaccgtcagg gtgtttacaa cggcagagat aatttatcgg aaaacagtta
     2821 tcgcattggt gtgtcattta aactgtagta gacaggagac agtcacaatg aataaaacaa
     2881 taacggcgct tgctatcatg atggcttcat ttgccgcaaa cgcgtctgta ttaccggaaa
     2941 ctcctgtgcc atttaaaagt ggtaccggag caattgataa cgacactgtc tacattggtt
     3001 taggtagcgc aggtacggca tggtacaagc tggatacaca ggccaaagat aaaaaatgga
     3061 aagcgttagc tgcattccct ggtggaccaa gagatcaagc aacctcggca tttattgatg
     3121 gcaatctgta tgtgtttggc ggcattggca aaaacagcga gggcttgact caggtattta
     3181 atgacgtaca caaatacaac cccaaaacca atagttgggt taaattgatg tcgcacgcac
     3241 cgatgggcat ggcgggtcat gtaacttttg tacacaacgg caaggcttat gttactggcg
     3301 gtgttaacca gaatatcttc aatggctatt ttgaagatct caacgaggct ggaaaagatt
     3361 caaccgctat agataaaatc aacgctcact attttgacaa aaaagcagaa gattattttt
     3421 tcaataagtt tctgttgtct tttgatccct caacacagca atggagttac gctggcgaat
     3481 cgccctggta cggaacggct ggtgcggcgg ttgtgaataa aggtgataaa acctggctta
     3541 ttaatggcga agccaaacca ggattgcgaa cggatgccgt atttgaactt gatttcaccg
     3601 gtaataattt aaaatggaat aagcttgctc ccgtctcatc accagatggc gtagctggcg
     3661 gttttgcggg gataagcaat gattctctta tatttgccgg aggggccgga ttcaaaggtt
     3721 cacgagaaaa ttaccagaac ggtaagaact atgcgcatga aggcctgaaa aaatcatata
     3781 gcactgatat tcatctttgg cataacggga aatgggataa atcgggtgaa ttatcgcaag
     3841 gtcgggccta cggagtatca ttgccctgga ataatagtct attgattatt ggcggtgaaa
     3901 ctgcaggcgg caaagcggtg acggattcag ttttgatctc tgtgaaggat aataaagtta
     3961 cagtacaaaa ctaacgcttc agggccccgg taaaggggcc ctgctatcaa tttgtcattt
     4021 caattacgcg aaatttatat gaacgcaata atatcgcccg attattacta tattcttacc
     4081 attgctggtc agtctaatgc catggcgtat ggcgaaggac tgccattacc ggacagggaa
     4141 gatgcgcctc atcccagaat caaacaatta gcgagatttg cgcatacgca tcccggaggc
     4201 ccgccatgtc actttaacga catgattcca ctgactcact gcccacacga tgttcaggat
     4261 atgcagggtt atcaccatcc tctggcaacg aatcatcaaa cacagtacgg caccgttggc
     4321 caggcactgc atatagcacg gaaattactg ccctttattc ctgataatgc aggggttctc
     4381 atcgttccgt gttgccgtgg cggatcggct tttaccgcgg gcagcgaagg gacatattca
     4441 gaacggcacg gagccagcca tgatgcttgt cgttggggaa cggatactcc gctataccag
     4501 gatttagtca gcagaacgcg agccgcactg gcaaaaaatc cgcataacaa attcctcggc
     4561 gtatgctgga tgcaaggcga atttgactta atgaccagtg actacgcgtc acaccctcaa
     4621 cactttaatc atatggttga agcctttcgt agggatctaa aacaatacca ttctcagctt
     4681 aataatatta ctgacgcacc gtggttttgc ggcgatacca cctggtactg gaaagaaaat
     4741 ttccctcatg cgtatgaagt tatttatggc aattatcaaa ataatgtttt agccaatatt
     4801 attttcgtcg acttccagca acaaggtgaa agaggactga cgaacgcgcc tgatgaagac
     4861 ccggacgatt taagcacggg atattacggt tcagcgtacc ggtcaccgga gaactggacg
     4921 acggcactgc gaagcagtca tttcagcacg gcagcccgtc gggggattat ttctgacaag
     4981 tttgtagaag caattttgca gttttggcgc gaaaggtgag cgttcattaa tttgatttat
     5041 tcgcatccat cagcgctttt aactgctccg gcgtaaacgg tggtttgcgt cggtgcaaca
     5101 tcgcatggca attagggtac acaggcagca aatcattaac tggatccagc tggtaatcct
     5161 ctttaatggc tgacagcggc accaggtgat gcacatggat aaagccttca gcgatatcac
     5221 catagatctt caccagatca acaccgcata cgttgcactg gcatccatga tgttcgaccg
     5281 ctttatctcg ggccttttta tcgcgttcat agcggtttac tgtcaccttt atcgccgcac
     5341 cttcaaggta tgtctcctgc gacggcaact catccgggaa cagaacgtcg gctatgggag
     5401 ccgcagcggc tttatccaga tgaatatgaa atccctgctc tatgagtagc ttgacacatt
     5461 tagattttat tccgccggtg aaatccgcag gggtaaattg cgtccccgtc atcaatgtcg
     5521 ctgcgatgcc agcaatcgcc ttcgaggggt aacgtcggcc atgatattca atctcataca
     5581 accgcgcagg ctggaactga tgagtttctc ccgcatccca taaatggata gcttcgatga
     5641 tataccgccg cggaagatga tcagggagtt tattcatgga gaatgtcctt atacgttctc
     5701 agaacaggca gcaaaaaaac aaatgagatc aaggtaatat atcttcactt gcaggcttga
     5761 acagcagaaa atttgcgagc atgatcgcaa ttatttcact gccagggata tcaatagctt
     5821 ccagtaaatc gctacagcag gcaattgcca atataaaaat ctggcacaag ggtgaacagc
     5881 gcgcgccgca taagccattg ttattgctat acgtattagc gggatacctg aatggacatc
     5941 cgcgcctttt cgattattgt agtggaacag tctacgagga tgcagttcga agtgagtaag
     6001 taaaggttgc gggaaacgat caatgccgcc atacaatgat tttttaggat caacaatgag
     6061 taataaaaac tacgaaagtc accgcaaagc gattgttagt aaaggtatac cacccgctct
     6121 attaaatagg ctcaccaatt cggatgttca ggtgatcaat accttcttaa cacgagtgag
     6181 caaactggaa ctatctcaac aagagaaaga ctggatcata aaaatcatct ctatggttta
     6241 gacaatttca gccttgtagc ctttcaaggg ttttgtgtgg gtacaaacat tatcgaatcg
     6301 gggtatagcc gcagctgtag ctgtatccac atcagtaagt tgatgtaggt tgacttaggt
     6361 tgagcgaaaa atagcgaaaa gccttgtgtg gcgcggattt cagtcataaa aaaacgtccg
     6421 ttgatgtcta ttgatgttcc gatggtgcga aggtgcgaag gtgcgaaggc cggactcaaa
     6481 catgtgactt aacctcatga tttaaaattg ttaataataa acaatatcat cttataccaa
     6541 cacagatacc aacacgaaaa atcactggtt ttcggcatcg aaccaggaca atcagctcct
     6601 gattgagata ccgagctctg ttactcgtca tcttcctcga caggcttcat tgcatcaagt
     6661 agtactcgca ccatcgcgat agaaccaatc cggataaaag catcttctcc atctatatct
     6721 gttaactgat atcgctcact tgatgccgga taattgtgtc gatttgcagc agtgaactct
     6781 ttcgtacagc cagaatgcgg cggatgctgg ttgagcagat aacgtccgta ttctcgttga
     6841 ccacttccgg acggtaggct gtgatatcag acataatcga tattcctttt tgcagataaa
     6901 tgaaaatgcc agccctgttc aggctggcgt cagtggaatg aagtctggtg agtaagactc
     6961 accggttctg gtgctgaagc tgcagttggc gcagggtacg gatatcctct ctggcctgtt
     7021 cgatacaggg caacagtgct gctgaatccg ttttatcctc attgttgtca aagataattc
     7081 ccgattcgca gtcgatattg tcctgcagcc acgcaatcag aatatccagt gcggtttccg
     7141 tggttaatga tttcatgttg ttcctggtta ccagttgaaa gtgggtaaac ccggcaggca
     7201 ttcagcgctg gcatccagat gaatgagact gacgccataa cgtcgggtaa gtgtgataac
     7261 cagacgacgg aacgtttttg acagccccag gcgtttaagc cgcagaaccg gatatgacca
     7321 cgcatcggta cgtaacagat atccgctacc tgtgaaatga atccattctg attcaccaac
     7381 gtcactggtc tgatgcgaca gcgagtacag ccaggcgtta tccttttccg tgatatgtgc
     7441 ggtactgcag cgtatgccgg caagggttgc aaacggtggt agtgtgcagg ttgactgttg
     7501 gtcggattcc tccaccacgc ggagtgaata accgttttca gcgaccttgt taatcagttc
     7561 agtgagatta acaccatcga cgtcaacgac aattcgcccc atgttcagtg cctgcacatt
     7621 aattctgtcg gcttccagca tcagggaaag tttcatcgtt tctccggatg cttacccagg
     7681 gtaatgttat ttaccgttct gtaattgtcg cgggtcatca ggccggtcgc cctgcgagcc
     7741 cggaggatat caatactgtt tattaactga gagcggggac aagtgctgaa tcccggctgg
     7801 tcggtacgca ccagcgcgta tttttccacg agaaaattca ccgcatcaca cagtgaaatg
     7861 ccagcttcaa tatgctgctc aatcacacgt tcatcggcga acggtgtgtc attcagtgta
     7921 aggccgtaat gctggcccag tagtcgggag agcagagtct gccagatggt gacgggagac
     7981 gggcagctcg atgcctcccg tacgtgtgta tcgggtaatg ttttcatact gaaggttttc
     8041 ctgatatgca gagatgaaaa atggaaaagt ggcgtggtga aagcaccagg ccggagcaga
     8101 aggttattcc ggtggtttac tttttcgttt cgggcgtcgg ataaacagcc agataaacgt
     8161 aaccaccgct accgagagtg tcagctttgc aggtcagacc ttttgcatac agcgtgacgg
     8221 tatgctgatg gcggggattc agttcaccgc tggtgagcat gagttccagt tgtttcatca
     8281 gcagcggaaa ggcctggtcc aggtggtacg catctgcatc gctgaacagg cctctgatac
     8341 cggcgcggtc ggcaaggtaa tgcaggcggt taccctcctg caccagacgg gcgccgaaac
     8401 agggcgtcac tgtgcagggt agtccccacc agggatggtc gtcattgtca tcgggatgcg
     8461 ttgtcccgga aagtgtgtct gacacgataa aatcctcaca gaaaatcggt gaatgatagt
     8521 ttaacgatga cgggtcagcc ggcgcgcaga ccggcttcgg taaaggtgta ttcgttgatg
     8581 agcaggacct catccacagc ggcgtctgag ctcagccagt cgtactcgtt ttccgggtga
     8641 cggaacatca gttcagggtg acgctcatca gccggacata cggaccaaaa ctgtccttac
     8701 ggcgttctgc aaacacggcc agcacaccgg gaatatcctg cacttcacga ccggtatacg
     8761 cttcagcact gccgtgccag cggtatttgc cggtacagaa cggaaaaaga cgggatgccg
     8821 gatgctgacg gtgaatacgc atggcttcgc cacgggtgat gattttcata gtgggatacc
     8881 tctgaagaca gaagataaaa gtgaaaacag gtgtgatgtg gttgtgacgg gttaaagcag
     8941 accatgttcg gcaaaggaga aaacctggct gccaccgact atcagatggt ccggcacccg
     9001 gatatccacc aggcccagtg cctgtaccag acgttccgtg ataaggcggt ctgccttgct
     9061 gggtgtgact tcaccggacg ggtgattgtg tgccagcacc acagcagcgg cattgtgata
     9121 cagggcgcgt ttaatcactt cacggggatg gacttccgtg cggttgatgg tgccggtgaa
     9181 gagggtttca ccggcaatca gctgattctg gttgttcaga tacagcaccc ggaactcttc
     9241 acgctccagt cccgccatgt tcagaatcag ccattctcgt gccgcacggg tggaggtgaa
     9301 ggccacgccg ggttcatgaa gatggcggtc cagggttttc agggcccgtt gaatgaggct
     9361 gcgctcgccg ggcgtcatct ctccgggcag aaaggaaagc tgttgcattg tacttctctc
     9421 cattcagtcg atgatgcgca taatggcgct gcattccgga tgctgcaggg cgtaatcccg
     9481 cagccggtaa taatggaccg tcatggcata acactccgta cgacaggcat gatgactgta
     9541 cgtcatcaga caggcagcaa tgccggcagc ttccggactc atttcagcgc ggttaccgtt
     9601 catggcattg aacagtaccc atgtttcgtc atcatcgtca tccggttcgg gtgccataaa
     9661 tgccccgccg ttgttcaggg tgtacagatt ccagatacca ccgcagtagt cttcacacag
     9721 acggtccatc cagccgaaga cacggggctc cagggtcacc cactgtggaa tgaggccaaa
     9781 gtgctgcggc cagaagctga tgcgctgttc atcagggacg agggtggcaa ccaactgagg
     9841 ctggtcattc cctgatgcag cggttacgga aacagaagat gtggtggaat tatgtgaaat
     9901 ggttttcatg aaatgactcc ttaaaggaca aataaatgcg tggttttcag ggtaatggct
     9961 gaatcagcgc aggctctcca gcagggtttc cgccatcacc cacaatacgc ggttgagctt
    10021 aatgtcagtg tcaatgctgt gaatggcacg ggt
//
LOCUS       DAZGOB010000157         9875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGOB010000157 DAZGOB010000000
VERSION     DAZGOB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.157"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            239..421
                     /locus_tag="ACHLIF_004376"
     CDS             239..421
                     /locus_tag="ACHLIF_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608267.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
     gene            complement(650..724)
                     /locus_tag="ACHLIF_004377"
                     /pseudo
     CDS             complement(650..724)
                     /locus_tag="ACHLIF_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(721..1146)
                     /locus_tag="ACHLIF_004378"
     CDS             complement(721..1146)
                     /locus_tag="ACHLIF_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9608268.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            1233..1448
                     /locus_tag="ACHLIF_004379"
                     /pseudo
     CDS             1233..1448
                     /locus_tag="ACHLIF_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1491..1830)
                     /locus_tag="ACHLIF_004380"
                     /pseudo
     CDS             complement(1491..1830)
                     /locus_tag="ACHLIF_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2146..2790)
                     /locus_tag="ACHLIF_004381"
     CDS             complement(2146..2790)
                     /locus_tag="ACHLIF_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608269.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(2811..3080)
                     /locus_tag="ACHLIF_004382"
     CDS             complement(2811..3080)
                     /locus_tag="ACHLIF_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9608270.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(3159..3797)
                     /locus_tag="ACHLIF_004383"
     CDS             complement(3159..3797)
                     /locus_tag="ACHLIF_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO9608271.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(3782..5005)
                     /locus_tag="ACHLIF_004384"
                     /pseudo
     CDS             complement(3782..5005)
                     /locus_tag="ACHLIF_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(5506..8187)
                     /locus_tag="ACHLIF_004385"
     CDS             complement(5506..8187)
                     /locus_tag="ACHLIF_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO9608272.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKFIREKQVSLNEYEEVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(8428..9474)
                     /locus_tag="ACHLIF_004386"
     CDS             complement(8428..9474)
                     /locus_tag="ACHLIF_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO9608273.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(9578..9727)
                     /locus_tag="ACHLIF_004387"
     CDS             complement(9578..9727)
                     /locus_tag="ACHLIF_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608274.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
BASE COUNT         2696 a         2444 c         2021 g         2714 t
ORIGIN      
        1 ggatgcaggt atcttctgat aatcatacat cccgaatttt gttcttaata gttgagtagt
       61 ggtatttggt ttccctgaga gaatataaag gtgaatcatg ctgcattgag tttgattcaa
      121 tttgtactga gacaagtatc ttcatgatta taatcaaaag ttgggtggat tggtctggcg
      181 ctcatcattt tttcgaataa actcactaat tattgccccc cccaaaggag atgatttcat
      241 gtataattgt ggtgaatcat attcagaata cgacatatca ttatctgacg atgaaaacta
      301 tgacgatgac tattatcgtg aacgcccgtg tccgggagaa gttttcggct atgataatac
      361 tgaggataat gatgaggcgg catctgatta tgcattaaaa ataaatgaaa actctgatta
      421 atacgggggt agctgataat aagacaatca atgacagata ttaatgaata aacataatca
      481 ggcgctcaat acttggttta atctgggcgc ctgattaaaa tctgaggaaa catataataa
      541 ttttcatcaa aatagtattt atgtactaac taagctggat ttgaacctct atttctgggt
      601 atttgttatc acttaatcca ttactgctcc tccttcagca tatgtcgtat ccgttgaagg
      661 atttacgcat atcggtaacg tcggcaacga gccagatgcg ggtacctgac gggagtgaga
      721 tcatcgtccc ctcccggtca gttcacggat caacagcgtg agcagctctg gcgatggatt
      781 ttccagcgtc atgttaccgt gacggaactc caccttgcag gagccggcac tgacactggt
      841 ctgagtggaa gtggataaag acggcgcaat ggccgccacc ggttctttct gctcatccgg
      901 cgttatttct acaggtaata attcaacgcc agtgtcagaa gaggtcgtta cctgaagacg
      961 ccgtgatata cgcccttcgt tctgccagag cctgagccat ttgaacagga ggttatcatt
     1021 gatatcgtgt tcccgggcaa tacgggcaac agaggctcct ggttgtgatg ccagttccac
     1081 catacgaagt ttgaattcat tcgaatagtt tttacgaggt tcttttcgcc agtcctgtaa
     1141 ttccatactt agatgtccgt ctatatcaga tgggcgtcta agttaccaat tttcgtctga
     1201 tggctacata cggcggtcag tttacgctta ctgacaacgc gatggcggag agcatcaatg
     1261 gtctttacaa agcggaggta atacaccgta agagctggaa aaaccgtgca gaagtggaac
     1321 tggccacact cacgtgggtg gactggtata acaatcgacg attgctggaa aggctgggcc
     1381 atactcctcc ggcagaagca gaaaaagctt attatgcttc catcggaaac gatgatctgg
     1441 cagcctgagt tcacagataa aacactctcc aggaaacccg gggcggttca aacctgattc
     1501 tggaaaagga ctttgtggat tgttgctatt gccagtgaga ttgaatgaag tcgttttaac
     1561 tcgctttgaa tacgtcttgc ccccagattt cgctgagaac gtaactccag aatcagagcg
     1621 acttcgcaag tgccggtttt cgttgatggt gaacgcttgg ggcgacggct gtggctttcc
     1681 agaccagcga tgccctgagc cagatatctg cgccaccatt ttacgcaatg tgggccgaga
     1741 aatgccacag cgacggcata caaatcccgc atcaccggac gtttcataga gttttaccca
     1801 ctgtagccgc tgctgaattt ccctgttcat aatttggcat tagttcagct caatcccgac
     1861 ttaaaaagtc cctacaggat gttccggacc tgaggcacag ttcatatcct tatccataaa
     1921 aaatttagct atgtctatag cattaatctt gacaagacga cgttagggcc tggcgatgaa
     1981 tcatttcccc tgccagatat cctttggggt gcaccagaac taccgtttta tatctatata
     2041 actgaaacgt ccctcgccat ccttcatatt ttgatgtagt aacaccattc actttcatct
     2101 accccccgtt ttattaacat atagtaaaaa tcacctcgcc gcattttatt cttttgaagt
     2161 aaaatcattt accagttcac ttaaagctct cttttgttca tcatcataca gagattgaat
     2221 ccggaattta atctcttctg caaccagtct tgcatcgggc tcataaaata tttttgttat
     2281 tcgctgtttc tgatgttcag gctggtcttc catatttcgt aaaaccagat gaaataatat
     2341 atcataaagt gcgcaatgcc atgaataacc atcattggtt aaaataagct cccaaacccg
     2401 ctcctgctcg gcaataagct gcagaacaaa ggactcaacc tgttctgttt gttcgggggc
     2461 aacacggtat agcggagcaa tcacagcaaa agatagttca tttaaccagg attccgcttc
     2521 cttctcgctc ctgaacggtg gacagttttg tattcttcgt tcgtattcac gggtaatctg
     2581 ctccagaata tcctccggcg gtgtcagcca cttattcctg agcaatacac tggttgatgg
     2641 atgacagctg gctatttcag caataatttc acgtaatgtc tccgggctga acttcttcag
     2701 ctgattgtag tgctcccggg taaaaccatg ttcatcgcat aaaagttgcc atgtcattga
     2761 tatactgttt gccattcctt gcttcatcat taatacctgt tacctgaata tcaacagacc
     2821 ggtgtacctt ttctgaatac ctccccggac ggagtggcaa tccccattcg cctcagcgtg
     2881 gcatctgcag ctttcgccac ctcgctgata tccagcgttt caggtagcgg cggggacgat
     2941 atctgcgcca gacaataatt ccggcgcaac aacaaccact cccgttgtgc ttctgtacat
     3001 tcaacatttc ccataacaaa aagccagcgg tcagaagctc tgcgccagca accagcagcc
     3061 tccagtttct gcgcaaccat gtcttccaca acttccgggg tactcttttt cctgctcaaa
     3121 acattaactc actgaaataa tatacatatc tgacggtctc agcgaaccgt ccatgccggg
     3181 ctgaaatcct cttcctgaaa cagctccgtc agcccgctaa tctgtttcaa tctgagaact
     3241 tcatcctggt ccatacccag ctccgtacca atgcgttcat tcgtccagcc caggcgcgac
     3301 aggtccctga caatatcaga cattgaggtt atctgatgtt tgcccctggc ccggttatgt
     3361 cggatggtcg cagcaatacg tgaggcctgt ccctttctct ccggattaat acagaccacc
     3421 ggcagccagc ccttcagacg ttttcccgta cctgcttccc tgcccagtac ctggcgatga
     3481 aaaccatcca ccaccaagta atggttcttt tcctcagaca ctaccaccgg ttgtgtgaaa
     3541 ccatctttct ccagtgactg cttcagcagt ttcttctcac ccggcgccat gacattagga
     3601 ttgtaatcat tggccaccac ctcatctgct tttacccaca gcacacagtc cacaggctcc
     3661 tgtgcaaaag gactgacctc atgaagcacc ctgcgcaggt gattaagggc ctgtatggcc
     3721 tcttcttctg gtcgttcctg gctgaagtac tgacgaatca tgttgccaag tatctccggt
     3781 gtcacagaat cccccattcc ttcctccttt ccttcatacg ctgcaggtaa cgttcatagt
     3841 gccggggttt gttagggcta aaggagaggg tccggcacca gaaatcattc tttatgagag
     3901 tcttacagat acgtcgccag gacggaatat cccggctccc caggtcattc tcctgttcat
     3961 ccggaatgtc atccgggaag ccccgcgtct gataccaacg caggtagaca gcaattttat
     4021 tacggtaatg ttctttccgg catcacatcc agcaggaaca tcgcatagct gcgccaggta
     4081 tgatgaggcg gcttcgtgat acgcttacac agggcaaaat aggcaccact ctcattggca
     4141 taaagcgccc cgctggcagc ccctgacacc cgttcacaca tcctggccca ggtttccggc
     4201 tccagaacat ggtaaagcca cagtccctta cgctgttcag gaccaaaagg ttcacagaca
     4261 cgcatgttgc gtaacggcac gccggcacgg tacatcaggt catacagagg attgtaaatg
     4321 gcacaggttc tggcgttata tatccagata tcgcgggcct tccagtcata caacggatac
     4381 atggtgtaat aaaacccttc aggcgatgcc gtggtccagg gcttatcatc ggcatatctc
     4441 agtttgcgct gagataccag tcccataaag cgattgagcg attcatcagc acggacacca
     4501 gtcagtattg ccactccaca ccggttaccg gcaaaccagg aagaaaatgc cggaacaaat
     4561 tcttcaaacg tcatggcata ccggtaaaag ggaaaatacg tcatgtctgt aatggcctct
     4621 tccggtggct ggcgaaccca ggtcactcct ggctcccagc atatccactc cggctgaaac
     4681 tgcgagacgc cgtttaccgt ggtcagggaa gtgccaccca gtaaaaggtt tccgtcacat
     4741 catggtacat ttcccgcatc ttctgaatat gttcaatagt gcaccgatac tgggcctccc
     4801 agtcaatgaa cagaacagag aaatgacgtt ttctcctgcg ggccacctct gccacaagat
     4861 ggaaaagcac agtggaatcc tttccacctg aaaaagacag gcataccgac ggaaatgttt
     4921 caaaaatcca ctcaatgcgg gcgattgcgg cacgcaaaac attttctccg gtaaggattt
     4981 tttcggttaa aagcgtatca ctcattttcc tggctttatg tcgaataatt ctgtttatgc
     5041 attcagacaa acttcattaa taactcaccg ctttggggat aaataataac aataaaactc
     5101 ttcagatatt aatctggttg ttggatttgt tatcacatcc tttatccacc tgcttcttac
     5161 tggtaacaca tctggaaaat ccatgcattt tcaacatata ccatcctttc aacttgtaaa
     5221 taaggaaata tgatgcagat ggtgaacaat tcaaatatca ttttgcagga agtattcact
     5281 gtaatagaat taccccctga taaagtttat tcactaagga attattcaac agaaccattc
     5341 cctgttagct ttgagcaata gcacttattt ttatatgtat caggaatacg tacgttgatg
     5401 taaccaaaac aaagaagcct gtgtccatgg ggttcgtaca attgtgtgtg gcacatgacc
     5461 aaatcaggtt tgagacctcc cgctgcaata ttttttattc agactttaac ctgtcaggcc
     5521 tgacagaaac gctcccaact tcccttctat atcactgtct atagtacgta atcttagcaa
     5581 tggcaggccg catttcttca gtatactgtt tttaagttca tcacgttcag cctgaacact
     5641 ggtcagatga taacctccat ccacttcaat aacccctaat ggagtcttcc cgaccttata
     5701 gtaaacaaca aagtcacaac tggcccggtt acgcatgaat aactgttcac ggtgtgtaaa
     5761 atccccccgc tccagcaaca ccaactgatt cagcgctatt tgtgaatgaa acatcatact
     5821 ccgataacta tcctgcgaca ggatatcccg caacaggcag gccacaatct gttctgactt
     5881 aaagtgtgaa tcatttgaat tcaatctact attcagtcgt tccagtgatt tgtcatactc
     5941 actgtacagc aaatcaaatg ctgatatgac cgggctctcg aaaatctctc cgtcatcagc
     6001 gtagtatttg atgtaacgaa tcagcgccgc aatatgtcca gcgtggcgtt caaatacatc
     6061 attaccagtc accagcgtaa atttatttcg ggcccttgaa acagcgacat tcaccagttc
     6121 aggattatca acaaacgcga tgttccgact gtgctggctg ctgcgcttct tatccagcac
     6181 agtggaaaac acaatttcat cgcactcccg tccctgaaat ttatgcaccg tcgatttcac
     6241 aaaatcagca ggcagcactg tttcagccag attaacctgt gcattatatg gggcgatata
     6301 tcccctgcta ctttcctcat cccagtaatg tccttccaga gattcaagct ccctgagatt
     6361 tgaaaaatta cgggtatggt ttccttttgc cgtgatgatc agagataatg aggcttcacc
     6421 tgaatcaagc gttagcggta tcagagcatt atcataaaac tgcttattac aaaattgaat
     6481 gattttagga tgacagcgat aatgctcttt aagaagagtt actggcacca tattcctgaa
     6541 caacatacaa accgaatcaa gtagactata tttctcacaa ttatagtatt ctgccggtgg
     6601 agatgggctg ttcgggagta atacaggaac atgtggaagt tgctttcgat cccctacaac
     6661 gattacatta cgggcacatc ccagtcccag aatgcccgga acaatatcct gctgtgaggc
     6721 ttcatcaata atcacataat caagaagagc ccccttaccg atagagttaa taatagaatg
     6781 cgtgctgcta ccaataaccg gaaaacgctt tataaaacgg ttaaattcat ccctgtatgt
     6841 ctctgccgag aatgaatttc ttgttgaaat atttcttcga agatgttgtt ttaaataaag
     6901 cacagaccat gatgtcaggc gctccagtaa aattttaaaa tcagattcct ttaatacttc
     6961 ctcatattca ttcagagaaa cttgtttttc ccggatgaat ttttcataat aagaaagctg
     7021 aagagaagta aatagtgcca gccttctctc cggaatcacc aatggtttga ctttcagtat
     7081 cctgaagttt aacaacaaat caattctgtt tctgaatcca atccgcctgt cagaaaggta
     7141 atgaatataa gccatcaggt cagttgtttt tcgtgaggaa agtcggtatt tgtgagaaaa
     7201 aagctcctcc ggtcgaagat tctgactctg ccattgctgc aggtatttat actcaatatt
     7261 cagctcatta atttctgctt ttaacgaagc gacctggtta atggcattca ggtgtttttt
     7321 cacttgttga agtacatcat caatcgcatt tgcagacggt gagtcaggta agacttcctc
     7381 agaacgcgaa atcgacgttg aaaaaaactg ttgcctgttc tccgtactcc ccagtcgtgc
     7441 caccacatat cccagttgct gtttgtccat cttttcataa acattactga cagctgagtt
     7501 attattggac aaaattgcca ctgttttatt ctgaattaat atattagcaa caatattgag
     7561 tatagtctgt gttttacctg ttccaggtgg tccttcaata actgaaacct gtgaggaaaa
     7621 tgcccgttcg acagccagca actgactttc attaagacca aacggaaaaa tcaaacccca
     7681 tggcgaatca cgttttttac tctgtcctga acagtatgca tgcaaggcgg tatccggatg
     7741 aggaactatt ttctttatct gcctaaggat attctcatcg atatttctgt tattaccgcg
     7801 ggcataaaaa atacgttcat tagccaccct acacaagtaa ttaaacacgg catgttctgt
     7861 aaatgctgaa cactgttgaa agtcaagacc ggtcgttttc atgatgtagg gtcgttcacc
     7921 ctcgcgataa taaacagcag catatttatc accatatata ataaccagtt cagcccggga
     7981 tgttacagcg ttccctttcc tttcgaggaa aacatttcgc aaatttacag ttggcgttat
     8041 ctgacataca gaaagcggac gagtatattt tctcccgctg cgaaaatgac aggtcagcat
     8101 aacctctctg tcgtcatttc cagcccacag cgtccagtcg gtaattttat gagtcttatt
     8161 ttcaccgtca acacaaatcg tgaccatatt cttactcaga aattatcatt taacttacgg
     8221 aaaattgtta aggcactccc gatattagct ccggaggatt ttccacaact ccgggactgt
     8281 taacatattt ctgcctgcca tatatacatt gaatttagtc ataacagtca ataccagaat
     8341 tacaggaatg cgatgccgat acgctggtta ttgaatactg gtagcccctg agcactaatt
     8401 catcacgata ctctgtattt actgttttca ctgcttttta caaccattcc cccaggtaaa
     8461 atgctgctcc tccggtgatg gctgtgtcag aaactgccat cccggaatag ccacgctgac
     8521 cgccagcaat gccgcacaga caccagccac aaacggtttc agaaacgtgg gcggggattt
     8581 ctgaagggag ggtgactgct ccgcctgcac tttccccttg cgattatcaa ggctcagtgc
     8641 atccagacgc accgcgccat ttgccgcaag cagcccttcc cggttatcca cgcttcccgg
     8701 cagactcagc gaaaaatccc ctgttcccgt ctgaatcaga ctgcctttac gatttgacag
     8761 gctttgtgcc gatatctgca gtgtgtcggc atttatcgta ccgccattac tgctgaactg
     8821 cccggcagtc cgggcggaaa gcgtatcggc actcagttgc gcattctgtg tagacagatt
     8881 tccactggtt gcattcagct cagtatgttg tccctgtatc cggctgttac ctaaatccag
     8941 cccctgctca atgtcccgta aatccagcag agaaccttcg tgtgccggga atgaggaata
     9001 caactgaata tagtcatcac tgtccggtgt cagacgcaca tgtcgcacaa caccgggaat
     9061 aataaccagc cggaggatac cgctttttaa atcctgcgaa ggtgccagaa cacgggtggt
     9121 gacataacca tgatcgacca gacggttctg caatgtactc atcagcagat taattccttt
     9181 cgcccccagg caatgcccga ccgccccgtt ggcgattttt tgtaaaggca gccagtgtgg
     9241 taacgcatca gcccccttca gttccgtctg tttaatctga aaacagggcg tttcaacagg
     9301 aaaattaatc ttatgggcaa aagagccagg tgcagaaagg cgaacatcag gggctgaagg
     9361 cgtcagttgt tgctcaaggg cttcctgacg ctgctgctga atgataaatg cttcatctcc
     9421 gctacgccgg acatccgctg cctgaagaga taaaggcgat aataaagcag acatcagtgt
     9481 cagtgacagc aaggacggac agcgccggaa taagctttgt tcctcagtcc tctcgttcct
     9541 gcaacataat tttccagtca aacgaaaaac cttttattca tgtcatacca acaatcacca
     9601 gcctcctgac gatacctccc accatgtttt gcgatggcac gctaccgccc ttccggtatt
     9661 tacccgcatc aaccggtacc agaatgctga gtaacaaacc agctagaatt gacttaattt
     9721 tgatcattat agaataatga tctgaactac agcaacatga cagatttatc gatcgatata
     9781 acacaaatga acagttcttt attctgacaa atatcgcaaa aacagcacat tacaaaattt
     9841 ataaccataa ttactggaaa agtgcagaaa attat
//
LOCUS       DAZGOB010000158         9814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGOB010000158 DAZGOB010000000
VERSION     DAZGOB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.158"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..574)
                     /locus_tag="ACHLIF_004388"
     CDS             complement(<1..574)
                     /locus_tag="ACHLIF_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HFO9608275.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLYIKISLI"
     gene            complement(571..2259)
                     /gene="yfaA"
                     /locus_tag="ACHLIF_004389"
     CDS             complement(571..2259)
                     /gene="yfaA"
                     /locus_tag="ACHLIF_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO9608276.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(2408..5035)
                     /gene="gyrA"
                     /locus_tag="ACHLIF_004390"
     CDS             complement(2408..5035)
                     /gene="gyrA"
                     /locus_tag="ACHLIF_004390"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO9608277.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5182..5904
                     /gene="ubiG"
                     /locus_tag="ACHLIF_004391"
     CDS             5182..5904
                     /gene="ubiG"
                     /locus_tag="ACHLIF_004391"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO9608278.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(6033..9767)
                     /gene="yfaL"
                     /locus_tag="ACHLIF_004392"
     CDS             complement(6033..9767)
                     /gene="yfaL"
                     /locus_tag="ACHLIF_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO9608279.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         2287 a         2727 c         2370 g         2430 t
ORIGIN      
        1 taattaatga aattttaata tacaacttcc atgtcataag ttgttgcaga ctgactgcgc
       61 gcattccgtt gtcagttttc gtggcgcttc cggtgtggta gatgacgtaa cgccccatcc
      121 agaccattaa gtgctgggca tcgccctgat cgaaaaaaat catatcgcca ggcagcgcct
      181 ggtttatgtc ctggccaata aactggctgt tgtactgaat caaattaatc gcggtcacgt
      241 aggggccggt tttcccgttc ccctgattcc agttttgcgc cagttgacgc tgtccaggtg
      301 ttagcgtcat ctctggcggc aaatactggc tagagaaacc gttacttttt agccatttac
      361 tatcgtgaac tttcagcgtc tcgttcgccg caaatcgcac caggcccgca caatcctgct
      421 gataccagcg tggacttggc ccctggcgga gctgttcttg tgcaatgcgt acaaaccagg
      481 cgcgaaacag cccggattgt tcgacattca gcatttcgct atgggcaaca acacaacaca
      541 gccagcaaat cagcgccagc agcccgtgcc tcatagcggc tgccaggtta tgggtagcca
      601 ctgccaggcg gcaccgggtt ccatctgggc cagcttcatg acataacgcg gttgttgaga
      661 taaagcgtcc agcttcggca ttaataaagt ttgtgcggcg ttataaaaaa ccggttcgag
      721 attcttcggc agactggtca acgtttcgtt acgcagcagt ttcgccacgc cttgtggatt
      781 gatatagagc ggaacgatgc catcagtggg tattacatcc accattgccg ggcggttttt
      841 attcagtgtt tgcagggcgt tattaaccag cgtgtcatcg agggagaaaa gcagcgtttt
      901 gttttgcatc gccagcgaca cgcggaaaaa ataatccgac attaattgat cgggttgcgc
      961 cgcctgcgct ttcggatact gaccgtatcg ggaactcact tcgcgacgcc agatttgcgc
     1021 ttcgccctgc tgagtctggc ttatcggcaa aacaccttct ggcgctttgc tttcgtgcgc
     1081 accaatattt tgcgtaaaca gtttccctgg caattgcgcc tgttcggcag tgccatcaaa
     1141 ctgaccgaca aacagcgggg tttgcaattt tgagtctgca taccagcaca gccccgcggc
     1201 accgtctaac gccccattca acttgtcgtt ttcctgactg atgtgcgaaa gcatctcttc
     1261 ggcaataccg tgtgaatacg gcaccaccac acagaagctg gccccggcag gcatactgtt
     1321 ccagaccggc gtaaaatcga aactggtatc tacgctggcg gattcatcat ttaacgccac
     1381 aaaactgtgc cagccgtcgt tacccatttc gaagcgtacg ccagcaaaag aaggcattaa
     1441 ccgctggtag ccaaaaccca gcaacctggc gctgaccacg atgcgctggc gtactggcgt
     1501 tttttcagca gtacgctctt ccaggccaaa gcttgcttgc cagcgttttt tgccgctcaa
     1561 caaatcacct gcgatcgccg tggcttcggt atcctgctga tcatctttaa acaacatatc
     1621 cgtgctggaa aacaccagca tcttgtcctg ataagtcgcg aacatcaggg cgttattgcc
     1681 gttatagcgc aactgataaa caggaacagt ttcactattt atcttgatgc tactgatttc
     1741 cgttttgctt aactggctgt cgctggtagc ggcaaacaac aatggctcca gcagtttgct
     1801 taaaccactg cgctggatca gcaccatata atgtgaaaga tggccttgtt tatcgtgcca
     1861 cagcgctgcc tgcgcgggct gatctaagag tgacgaaaag agcttatctt tcagcgtgag
     1921 atcgtgttca tagacaatac gacgaatgct gccttcaatg cccagacgat cggcatgatt
     1981 ctgataatag aaaacgaaat cttcgctcag aacatcgtgg agaaacggaa tggtgaggag
     2041 atctttggga agctggctca gagagtcgct gtcgagaaag aggtccggct cattgagatc
     2101 gatttgcaga ttgttgtgca ccaccagcgg cgacaacgtt ttttctggcc cactgccagc
     2161 atattgcaac gcccagacgc cagcggaaag cagtgctatt gcgccaaaac ctacaagacc
     2221 atagaaccgc cagcctttcg cctttttttc accactcatt gccacattcc ttgtgtatag
     2281 ccagccattt tttacgggca cagccaaact ttaccgtgcc ctaatacgac aaaagcccag
     2341 actttgcagc ctggactttt caattcaaac aagggagata gctccctttt ggcatgaaga
     2401 agtaaaatta ttcttcttct ggctcgtcgt caacgtccac ttccggagcg atttcatcgt
     2461 ccccttccgc ggcactgcca tcgatggtat ccagatcttc ctcgtcaacc ggttcagcaa
     2521 cacgttgcag acccactacg ttttcatctt ccgcagtacg gatgaggatc acgccctggg
     2581 tgttacggcc tacgatgctg atttccgaaa cgcgagtacg taccagcgta ccggcatcgg
     2641 tgatcatcat gatctggtcg cagtcatcta cctgtaccgc gccaaccact aaaccattac
     2701 gttcagtaac cttaatggag ataacccctt tcgtcgcacg cgacttggtt gggtattccg
     2761 ccactgcggt acgtttaccg taaccgtttt gcgttgcggt gaggattgcg ccatcgccac
     2821 gaggcacgat cagagagacg actttatcgc cttcacctaa gcgaataccg cgaacaccgg
     2881 tggtgttgca gcccatcgca cggacagaag actctttaaa gcgcaccact ttaccttcag
     2941 cggagaacag cattacttcg tcttcgccgc tggtcaggtc aacgccgatc agctcatcgc
     3001 cgtcaaccag tttgatcgcc actttaccgg cggtacgcag acggttgaac tcggtgagga
     3061 cagttttctt cacggtaccg ttagcggtcg ccatgaagac tttcacgcct tcttcaaact
     3121 cggtcactgg caggatcgca gtgatacgtt cgtcctgctc cagcggcagc aggttgacga
     3181 tcggacgacc gcgcgcgcca cgagtggctt ccggcaactg ataaactttc atcgaataga
     3241 cgcgaccacg gctggagaag cacagaatat ggtcgtgagt gttcgccacc agcagtcggt
     3301 cgataaagtc ttcttcttta atacgtgcgg cagatttacc tttcccgcca cgacgctgcg
     3361 cttcgtattc agaaagcggc tgatacttaa cgtagccctg gtgagagagc gtcactacca
     3421 catcttcctg agtgatcaga tcttccaggt tgatgtctgc gctgttggcg gtgatttcag
     3481 tacgacgttt gtcaccgaac tgttcacgaa ccagctccag ctcttcacgg atcacttcca
     3541 tcaaacgatc ggcgctacca agaatacgca acagttccgc gatctgatcc agcagctctt
     3601 tgtattcgtc gagcagtttt tcgtgctcaa ggccggtcag tttctgcaaa cgcagatcca
     3661 gaatcgcctg agcttgctgt tcggtcaggt agtacagacc atcacgcacg ccgaactctg
     3721 gctccagcca ttccggacgc gcagcatcgt cgccagcacg ttcgagcatc gcggcaacgt
     3781 tgcccagctg ccacggatta gcaaccagcg cagttttcgc ttctgcaggc gtcggcgcat
     3841 gacggatcag ttcgatgatc gggtcgatgt tcgccagcgc cacggctaat gcttcaagga
     3901 tatgagcacg atcgcgagct ttacgcagtt cgaaaatagt acgacgggtc accacttcac
     3961 ggcggtgacg aacaaacgcc gcgatgatgt ctttcaggtt catgatcttc ggctgaccat
     4021 ggtgcaatgc caccatgttg ataccgaaag aaacctgcaa ctgggtctgg gagtagaggt
     4081 tgttgagcac aacttcaccg accgcatcgc gtttcacttc aatcacgatg cgcataccgt
     4141 ctttgtcaga ctcgtcacgc agcgcgctga tgccttccac gcgtttttct tttaccagtt
     4201 ccgcaatctt ctcgatcagg cgcgctttgt ttacctgata cggaatttcg tggacgataa
     4261 tggtttcacg accggttttg gcgtcaactt ccacttctgc gcgagcgcgg atatacacct
     4321 tgccgcgacc ggtacggtaa gcttcttcaa taccgcgacg accgttaatg attgccgccg
     4381 tcgggaagtc cggccccggg atgtgttcca tcagcccttc aatgctgatg tcttcatcat
     4441 cgatatacgc cagacaaccg ttgatgactt ccgtcaggtt gtgcggcggg atgttggttg
     4501 ccatacctac ggcgataccg gaagaaccgt tcaccagcag gttaggaatt ttggttggca
     4561 tgacgtcggg aattttttcc gtgccgtcat agttatcaac gaaatcgacc gtctcttttt
     4621 cgagatcggc catcagttca tgggcaattt tcgccagacg gatttccgta taacgcattg
     4681 ccgccgcaga gtcgccgtcg atggaaccga agttaccctg accgtctacc agcatgtaac
     4741 gcagcgagaa tggctgcgcc atacggacga tcgtgtcata aaccgccgag tcaccatggg
     4801 gatggtattt accgattacg tcaccaacga cacgggcaga ttttttatag gctttgttcc
     4861 agtcattgcc tagtacgttc atggcgtaaa gtacgcgacg gtgtaccggc ttcaggccat
     4921 ctcggacatc tggcagcgca cggccaacaa tgaccgacat cgcataatcc agataggagc
     4981 tcttcagctc ttcctcaatg ttgaccggtg taatttctct cgcaaggtcg ctcatctaac
     5041 cgctatccct ctactgtatc ccggattcaa aggtcgcaaa ttataacaca gccgcgcagt
     5101 ttgaggtaaa cctatacgct ttattcacat ccaatgcctg atatactcgt ttgtcttgcc
     5161 aattacggag tagaagtgcc aatgaatgcc gaaaaatcgc cggaaaacca taacgtagac
     5221 cacgaagaga tcgctaaatt tgaagccgtc gcctcccgct ggtgggatct tgaaggtgag
     5281 ttcaaaccgt tgcaccgcat taacccgctg cgtctgggct atattgccga gcgtgctggc
     5341 ggtttatttg gcaaaaaggt gctcgatgtc ggttgtggcg gcggcattct ggccgagagt
     5401 atggcgcgcg aaggcgcgac ggtgaccggt ctggatatgg gctttgagcc attgcaggtg
     5461 gcaaaactgc acgcgctgga aagcggcatt caggtggatt atgtgcagga aaccgtggaa
     5521 gagcacgcgg caaaacatgc cgggcagtat gatgtggtga cctgcatgga gatgctggag
     5581 cacgtccccg atccgcagtc agtggtcaga gcctgtgcgc aactggtgaa accaggcggc
     5641 gatgtctttt tctcgacgct taaccgcaac ggcaagtcat ggctgatggc ggtggttggc
     5701 gcggaatata ttttgcgcat ggtgcccaaa ggcacgcatg atgtgaagaa gtttattaaa
     5761 ccggcagaac tgcttggctg ggtagatcag acaagcctta aagagcgaca tataaccggg
     5821 ctacattaca acccgctcac taatactttt aaactcggcc ccggcgtgga tgtgaactat
     5881 atgctgcaca cgcagaataa gtgaggttgt tgtttggccg agccaatgcc tgatgcgacg
     5941 cttgccgcgt cttatcagta tctacaaatg ctccccgtag gccggataag gcgtttacgc
     6001 cgcatccggc aaccgtgccg actagacagt gattaccatt tcaccgtcat cgacaaaaac
     6061 cctgccgtct gggcaaaatc atcactccct ttctgccacg ccacgctgcc gcgcagggac
     6121 actcgctgac tgatattgcc cgtgactccc acttttattt caccccgtcg cttcaccgca
     6181 tcgtcactga tagtgctgcc atcttcttca atttccgtcg catggggatc gtgataataa
     6241 ttcagatcta atgttggtgt gacaccaacc gcggtacgcc attcgctgtg taaacccagc
     6301 cgcgtctgaa tatcatcccc ctgcgattgt gacacgcgcg catggttagc ggcggtaaaa
     6361 tcatcctgct gcacgccctg ataaatcacc tgcgcctgcg gttcaatcac cacaccacgc
     6421 cccggtaacc actgataccc ggcttccagc gaggcgataa tccccgacga gtggtaatga
     6481 tctgtgccat cgtcttgttc ggaaacatca ttgttaaacc acgcgtattg cagccagcta
     6541 tccagccagg ccccctgctt ctgattaccg tgctgatacc agcttgaggt cagcccaacg
     6601 gcataaccgt ggttctggtt atcggcgcga gttccggtca tattcgagcg gctctcgccc
     6661 tgattatcac tgtagccacc aaccgcccca agcatccact cgccatcatc gccccagcgc
     6721 ccgctaaaca ggtcaccgct aagctgcacc gtagaagtgt cttcatgctg agccagttgc
     6781 cctgccgccg tgtaatgata acgtccgccg ataacacgta aattcagcgt ctgaccattg
     6841 ccacctgcat gatcgcgtcg ctccatcata aacgcctgat ttgccgcccg cagattattg
     6901 agataaccgc caactttggc attcagcacc ggctggtaag cggggattgg atcgggtgtt
     6961 gggtcaggat cgggtgttgg gtcagggtct ggatcaggtg gcaatggtgg cgttacttct
     7021 tgcgatcgca gataccagtc gttgttatct tccaccagcg tgtagtcata tgctcccata
     7081 ttgacgtagc cgctgcctgc cagactgaac ttcgcattgt tttgaaactg cgtgggatcg
     7141 gctgcgaaat caaccacttt aatgcctgtc gatgtcggct caccaatccc tgtaatggag
     7201 ttaaccacca cagttgtgtt gccagcagta ttaccgttca acaccaattg atcgcttacc
     7261 gagtcatcgc cgtttaattc gctatcgagc agtagcgtac cgccgccggt ataatcaccg
     7321 tttaccgtca gcgtatcgcc agcgacgcca ttttgcaggc taaccgcacc ggacgtattg
     7381 gtcagattgc cattaaccgt cacgtcggaa agataggtca ggctgtcagt ttcaaggttg
     7441 gtattaagct cacctgtcaa cgtcacgcca tcggtggcat tcaccagcgt cccatcgccg
     7501 ttaaacagtg aggcattaag ggcgacagaa gtatcctgcc cggtcaggcg caaaaccgta
     7561 ccatcgctga tgtcgatagt gccgctggaa gtagcatcaa tagattgaat atcctgatcc
     7621 gcgccagtaa cgaacgtcgc cccatcaccg acccacagtg acgaagcata aggaaggata
     7681 tccgcgacat cgcctttcag ggtgccttct tcgacacgta ccgccgactg cgttgtaccg
     7741 ctggcggtca gctccagcgt acccgccccc gttttagtca atgtgctacc ctcatcctga
     7801 tgctgcccgc tgctgtcagc catcagtgcg ccccactgcg tgtctacccc cgcatcaact
     7861 gccacttcac cgtcggcgcg catttcaata tcacgcccat gctcagtcgt cgtagagcga
     7921 gtatcgacat tggcagtaat ctccatcacg ctttcttgtt gcttatcggt aaagataacc
     7981 tggcggttat agtgggtatc tccaagctgt gagttgtcgc tcacctccag cataccggag
     8041 gcgatttgtg ttgtgcccaa ataactatta ttgtttgcca gacttacctg cccgtcgccc
     8101 gcatcgacca ccacgtagac gccatctttc ccgtcaccgc cgatatgcat atcgtttcca
     8161 cctgcaagat ctaccacatc ctgactgccg ataaccgttc cgctgctgcc gctgacttca
     8221 aggccatcgt tgtatgatgt gccactctgc caggtggaga aatcagagag atcgagcaca
     8281 ccgccgttta acacgatcga ctgcggatcg tcctggagag cggtaagatc tgccgcgtcg
     8341 ccttccagcg aaagcaccgc accatcatca acgactatat cgccggttag cgccatcgac
     8401 tgcgcccccg acagcacgta gctgccgttt tgcgcaatgg tgagctgacc agcaccttcg
     8461 atggtgccag caaaactgcc ttgattaaca gtaacattgc caccagcatc gatatttaaa
     8521 gtgccattct gaaagcccgt caatgagtgc acaaaggttt gttgggtgga gccaacgttt
     8581 agttctgcct gattctggta cttatcaata ctccctatcg tcagaccgta gcagtcttgc
     8641 ggatcgtcct ggcaatgcgt atcgccgaca ttcatcaggg agttgctgcg acccagggta
     8701 acttcaccgt tttcaatctg catctcacca gtaaagtcat tgttatctgc attaagtacc
     8761 agatcgccgg aacctgtttt ggtgatcaac ccggtaccag caatagagtc aacagctccg
     8821 tcattctctg tattgccaat aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca
     8881 cttaagccga gatacataaa gccacccgcc gcagtggaag gaccatctcc atagcctgct
     8941 gcgctgttgt tctcatccac taacacgccg ccgttctggc tgtagctgtc atcaacagaa
     9001 atatcaataa gatagggagc cgtcgcgcta ttggtatata tcgccccgcc ataaccttcg
     9061 gcagtgttat ttgtaaaggc ggtgttattt attatcgtat aacctgaagg atgcttgctg
     9121 tcgctattat tatcggtaac atcgattgcc ccgccatcgc catcactgta acttgttgat
     9181 gtatatgcct ggttgttatc aaaaataaca tcacttaaat aaacgtcatt attaatggta
     9241 taaattgcgc cacctttgcc atcattagcg atgttattgc gaaacatggc gttagtgaca
     9301 cgtaaatcga cgacaccagt atcattagta ccagaagaat agattgcgcc accatagccg
     9361 cctgcgacgt taccagaaaa aataacatca gtaagattca gcgttgagtt ttctttagca
     9421 aatatggccc cgccgttatt atattctcct gtaacggtgt tattggcaaa cagagtcatt
     9481 ccagtttcat tttctggtaa tagtgaaaat tccgctcctt gttgcaaaaa tacggctcct
     9541 ccgctggcgt tattggtcat atccgaaaaa accagccatt gcccatcggc gacactccag
     9601 tcctgcgtaa tgcctgaaag gctttgcctg ctggcgtgac aactcgcttt gatatcatat
     9661 ccctggcata aatcaatggc cgccgcgaaa ccgttagcag agaaaagaga tgcaatcatt
     9721 gacgggagta gagataaata ctccttgcgt agaaagataa tccgcatatt catctaacca
     9781 tcattttata taagacggca tatttaaacg catt
//
LOCUS       DAZGOB010000159         9786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGOB010000159 DAZGOB010000000
VERSION     DAZGOB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.159"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1058)
                     /locus_tag="ACHLIF_004393"
     CDS             complement(<1..1058)
                     /locus_tag="ACHLIF_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9608280.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSGNRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLADISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKLAIPLTLTIDALNISLADTLQKCREASGSETIIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREWDK"
     gene            complement(1036..1254)
                     /locus_tag="ACHLIF_004394"
     CDS             complement(1036..1254)
                     /locus_tag="ACHLIF_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9608281.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTSSLLKRIRNGKKAKS"
     gene            complement(1360..1704)
                     /locus_tag="ACHLIF_004395"
     CDS             complement(1360..1704)
                     /locus_tag="ACHLIF_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608282.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(1823..2065)
                     /locus_tag="ACHLIF_004396"
     CDS             complement(1823..2065)
                     /locus_tag="ACHLIF_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9608283.1"
                     /translation="MFALIQRGQIYTDSAGYPIKILRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQENHLKKLRAMQRR"
     gene            complement(2140..2490)
                     /locus_tag="ACHLIF_004397"
     CDS             complement(2140..2490)
                     /locus_tag="ACHLIF_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9608284.1"
                     /translation="MINRTKLEHILEYARQQRRFGQLCKILPGDMVEIVEIAMRKTGN
                     SPVSPGGWISCSERMPAQDDWILIYSKHGEYMAGQVQEEYVELSDGTLSWLGNALYWM
                     LLPEPPQGVNDELA"
     gene            complement(2787..3092)
                     /locus_tag="ACHLIF_004398"
                     /pseudo
     CDS             complement(2787..3092)
                     /locus_tag="ACHLIF_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(3089..3310)
                     /locus_tag="ACHLIF_004399"
     CDS             complement(3089..3310)
                     /locus_tag="ACHLIF_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9608285.1"
                     /translation="MADIIDSASEIEELQRNTAIKMHRLNHQAISATHCCECGDPIDE
                     LRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(3409..3624)
                     /locus_tag="ACHLIF_004400"
     CDS             complement(3409..3624)
                     /locus_tag="ACHLIF_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9608286.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(3701..3892)
                     /locus_tag="ACHLIF_004401"
     CDS             complement(3701..3892)
                     /locus_tag="ACHLIF_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO9608287.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            complement(3865..4047)
                     /locus_tag="ACHLIF_004402"
     CDS             complement(3865..4047)
                     /locus_tag="ACHLIF_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO9608288.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            complement(4047..4724)
                     /locus_tag="ACHLIF_004403"
     CDS             complement(4047..4724)
                     /locus_tag="ACHLIF_004403"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO9608289.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            complement(4721..5506)
                     /gene="bet"
                     /locus_tag="ACHLIF_004404"
     CDS             complement(4721..5506)
                     /gene="bet"
                     /locus_tag="ACHLIF_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO9608290.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            complement(5512..5808)
                     /locus_tag="ACHLIF_004405"
     CDS             complement(5512..5808)
                     /locus_tag="ACHLIF_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9608291.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            complement(5863..6027)
                     /gene="kil"
                     /locus_tag="ACHLIF_004406"
     CDS             complement(5863..6027)
                     /gene="kil"
                     /locus_tag="ACHLIF_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO9608292.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIHYPPFLFS"
     gene            complement(5996..6160)
                     /locus_tag="ACHLIF_004407"
     CDS             complement(5996..6160)
                     /locus_tag="ACHLIF_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO9608293.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            complement(6233..6601)
                     /locus_tag="ACHLIF_004408"
     CDS             complement(6233..6601)
                     /locus_tag="ACHLIF_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO9608294.1"
                     /translation="MSNIKKYIIDYDWKTSIEIEIDHDLMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDPDDMTIKAA"
     gene            complement(6751..7221)
                     /locus_tag="ACHLIF_004409"
     CDS             complement(6751..7221)
                     /locus_tag="ACHLIF_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608295.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(7230..7322)
                     /locus_tag="ACHLIF_004410"
                     /pseudo
     CDS             complement(7230..7322)
                     /locus_tag="ACHLIF_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            complement(7355..7693)
                     /locus_tag="ACHLIF_004411"
     CDS             complement(7355..7693)
                     /locus_tag="ACHLIF_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9608296.1"
                     /translation="MLMVKKTIYVNPDRGQNRKVSDRGLTSRDRRRIARWEKRIAYAL
                     KNGVTPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIEDEKYEDVMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(7696..8001)
                     /locus_tag="ACHLIF_004412"
     CDS             complement(7696..8001)
                     /locus_tag="ACHLIF_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO9608297.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLCCKKKEEVVRKNRSIYYKDSNPLGNKIHAVQKIKLYSKLPYGAY"
     gene            complement(8316..8966)
                     /locus_tag="ACHLIF_004413"
     CDS             complement(8316..8966)
                     /locus_tag="ACHLIF_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO9608298.1"
                     /translation="MNTQLMGERIRARRKELKIRQAALGKMVGVSNVAISQWERSETE
                     PNGENLLALAKALQCSPDYLLKGEDSLSNIAYHSRHDPRGSYPLISWVSAGCWMEAVE
                     PYHRRAIDNWYDTTVDCSEDSFWLDVKGDSMTAPAGLSIPEGMIILVDPEVEPRNGKL
                     VVAKLEGENEATFKKLVIDAGRKFLKPLNPQYPMIEINGNCKIIGVVVDAKLANLP"
     gene            9047..9232
                     /locus_tag="ACHLIF_004414"
     CDS             9047..9232
                     /locus_tag="ACHLIF_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9608299.1"
                     /translation="MYKKDVIDHFGTQRAVAKALGISDAAVSQWKEVIPEKDAYRLEI
                     VTAGALKYQESAYRQAA"
     gene            9342..9638
                     /locus_tag="ACHLIF_004415"
     CDS             9342..9638
                     /locus_tag="ACHLIF_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO9608300.1"
                     /translation="MAQASYSKLTQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDMQF"
     gene            9671..>9786
                     /locus_tag="ACHLIF_004416"
     CDS             9671..>9786
                     /locus_tag="ACHLIF_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9608301.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEA"
BASE COUNT         2341 a         2386 c         2133 g         2926 t
ORIGIN      
        1 attttgtccc actcccgccc gcgactgtcc ctgtaacgcg ccgccattga atctgattta
       61 tgcccgagaa gacgttgagc aaacttatcg ccaatctgat tccggtatag cctcgccgac
      121 aggctgcgca gttcatggaa tgttggcggg tctccatcaa atgagagtcc agatgcattt
      181 ctcgcctttg taaaatactt agatactgtt ttcggggaaa gcggttcgtg atgcgttgat
      241 gcaattattg tttcactgcc gctggcctcc ctgcatttct gtagtgtatc agccaatgag
      301 atattgagcg cgtcaatcgt tagcgtcagc ggaatggcga gcttagcccc tgttttaccc
      361 tgctcaatgt gaagatggtt gtcgtttatg tctgaccatt tcattctgca taaatcgcct
      421 actctctgcc ctgtaacgac agccaaatcc attgccagcc tcagccagat tgggagcggt
      481 tcggctgcat ggtaaatcgc gacatattca ttagctgtca gtcttgaacg ccttacttct
      541 gattttgctg tgcgggttgc tgttaccgga ttcgtagcca catgcccctc ggctattgcc
      601 tcacgaaaaa cgtcaacaag ggttgacctg attaattttg cggaagccgc tttaccttct
      661 gctacgtagg tgtttagcat tgctgccacc tctttcgttg atatgtcagc gagcggtttg
      721 tccggcaatt ttcttcggat tgccctgatt ttgctggcgt agtcgagtag agttttcggc
      781 ctgatacccc tctcgctgag gattgtttca tatcggtcaa gccacgcatg aagagtgatt
      841 gcgtcagcgc ctttaattct gtctatcagt gactcacgcc tgttcccgga tagcaactca
      901 atattggcct gtattgcttc agtgattgct atcctcctgt ctcggcctaa tccgaactct
      961 ttacccgtcc ttgggtccct gtagcagtaa tatccattgt ttcttatata aaggttaggg
     1021 ggtaaatccc ggcgctcatg acttcgcctt cttcccattt ctgatcctct tcaaaaggct
     1081 acttgttact ggtcgattta agtcaacctt taccgctgat tcgtggaaca gatactctct
     1141 tccatcctta accggaggag ggaatatcct gcattcgcgc acccatcgac gaactgtttc
     1201 aaggcttctt gggcgtcgct ggcgtgcatt ccactcctga agtgtcaagt acatcgcaaa
     1261 gtctccgcaa ttacacgcaa gaaaagccgc attgatgcgg caatggtagg tctggatatc
     1321 ttgagaaatg aacaggcctc attgagtgtg aggctgtggt tagtccttgc gtagctcgct
     1381 aattcttctg taagtctctg gtgctttgtt tccgtgtatc ttcatttcag acttcaacag
     1441 agcgacgagg gaatcccatt cgttgaggat gcctttgaat gccggaacgc gctttgcaac
     1501 cttgtcgaat gaatctctga tttctggaat ctgctcaaca agtgcaacgc atcgccggaa
     1561 gtctgctgcg tcatgtggag cgccgaagtg atgaccatag atattctttt tcagtccaca
     1621 tgcgattgag gcaagagttg cgctactgat gccaacatcg ccagtcgatt gccatttcaa
     1681 aaccttcata gccaaatctg acatttcttg tctccataaa acaaaacccg ccgtagcgag
     1741 ttcagataaa agaaatcccc gcgagtgcga ggattgttat tcattgccga tattcacctt
     1801 tatcgcgaac acctttaccg gtttatctcc tttgcatggc gcgtaatttt ttcagatggt
     1861 tctcctgctc tgtttcagcc agaatttgtc ggtattcctg gtgatcgatc cgttcaaaca
     1921 gttcattaaa atcgtttatt tttaccgact gtgttcgccc atccattctt ctgtacaaca
     1981 cagtgttgtt tatgcagcga agaattttta tcgggtagcc ggcgctatcg gtgtatatct
     2041 gaccacgttg aatcagagcg aacattcctt tatccccagc ggaaaagcga atacagaata
     2101 aatgccaccg cgattgcaac tcctacagcg gtgaatgctt caggccaatt catcattcac
     2161 tccctgcggc ggttctggta gcagcatcca gtacaaggcg ttccctaacc acgataaagt
     2221 gccgtcgctc aactccacgt attcctcttg tacctgtcct gccatatact caccgtgctt
     2281 tgaataaatt aaaatccaat catcttgagc gggcattcgc tcactacagc ttatccaacc
     2341 acccggactt accggagagt tgccagtctt acgcatggca atctccacga tttcaaccat
     2401 atctcctggc agaattttac aaagctggcc aaagcgcctc tgctgcctgg catactcgag
     2461 gatgtgctcc agtttggttc gattaatcat gatttatctc ccttaagcat ggctgcacgg
     2521 caggcgttcc agcctctcac ctctgcaata gctgcaacag catcaactgt gtacatgcta
     2581 agagggttag gcattggttt ttcttccggt actactggcg atggctgttt ggcttctaaa
     2641 tcagcaattc tgtcaactac ggcatcgaca gcatctgaaa aactgaaaca gttactccac
     2701 tcaggcctat ccccggttgt tgcgaagtac atatcagcta aagcagactc agcatggtca
     2761 cgctcgttaa tgagttgctc ttcgcttttc tccagttcag caatacgttt actcccatcc
     2821 gagataacac cttcgtaata ctcacgctgc tcgttgagtt ttgattttgc ttcctcaagc
     2881 tcaacacgca gcttccctac cgttagcgca atttcctcgt tctcctggtc gcggcgtttg
     2941 atgtattgct ggtttctttc ccgttcatcc agcagtgcca gcacaatcga tggtgttacc
     3001 agctcatgga aaaggtccgc atcaaatccc cagtcgtcat gcattgcctg ctctgccgct
     3061 tcacgcagtg cctgagagtt aatttcgctc acttcgaacc tctctgttta ctgataagct
     3121 ccagatcctc ttggcaactt gcacaagtcc gacaaccctg aacggccaga cgtcttagtt
     3181 catctatcgg atcgccacac tcacaacaat gagtggcaga tatagcctgg tggttcaggc
     3241 ggtgcatttt tattgctgtg ttgcgctgta attcttcaat ttctgatgct gaatcaatga
     3301 tgtctgccat cttccattaa tccctgaatt gttggttaat acgcttgagg gtgaatgcga
     3361 acaataaaaa aggagcctgt agctccctga tgattttgct tttcatgttc atcgctcctt
     3421 aaagacgccg tttaacatgc cgatcgccag acttaaatga gtcggtgtga atcccattag
     3481 cgttaccgtt tcgcggtgct tcttcagtac gctacggcaa atgtcatcga cgtttttatc
     3541 cggaaactgc tgtctggctt ttttgatttc agaattagcc tgacgggcaa tgctgcgaag
     3601 ggcgttttcc tgctgaggtg tcattgaaca agtcccatgt cggcaagcat aagcacacag
     3661 aatatgaagc ctgctgccag aaaaatgcat tctgttgttg tcatgccggg tctctctcgt
     3721 ttgcttctgc tttcgccgcc atcatttcca gcttttgtga aagggatgtg gctaacgtat
     3781 gaaattcttc gtctgtttct actggtattg gcacaaacct gactccaatt tgagcgaggc
     3841 tatgtgccat cccgatactc gttcttaatt caacaggaga tgctttgtgc atacagcccc
     3901 tcgtttatta tttatctctt cagccagccg ctgtgctttc agtggatttc ggataacaga
     3961 aaggccggga aatacccagc ctcgctttgt aacggagtag acgaaagtga tcgtgcctac
     4021 ccggatatta tcgtgaggat gcttcattac cattgctccc catatacaaa accaatttca
     4081 gccagtgcct cgtccatttt ttcgatgaac tccggcacca tctcgtcaaa actcgccatg
     4141 tacttttcat tccgctcaat cacgacataa tgcaggcctt cacgcttcat gcgcgggtca
     4201 tagttggcaa agtaccaggc atcttttcgt gtcacccaca tgctgtactg cacctgggcc
     4261 atgtaagccg attttatggc ctcgaaacca ccgagccgga acttcatgaa atcccgggag
     4321 gtaaacgggc atttcagctc aaggccgttg ccgtcactgc ataaaccatc gggagagcag
     4381 gcggtgcgca tactttcgtc gcgatagatg atcggggatt cagtaacatt cacgccggaa
     4441 gtgaactcaa agagggttct ggcgtcgttc tcgtactgtt ttccccaggc cagagcctta
     4501 gcgttaactt ccggagccac accggtgcaa acctcagcca gcagggtgtg gaagtaggac
     4561 attttcatgt caggccactt ctttcctgat cggggttttg ctattacgtt gtgaatttct
     4621 gaagctgtga tgacgccgag ccgtaatttg tgccacgcat catctccctg ttcgacagct
     4681 ctcacgtcga tcccagtacg ctgcaggata atgtccggtg tcatgctgcc accttctgtt
     4741 cagtggcttt ttgtttcagg aatccaagag cttttactgc ttcggcctgt gtcagttctg
     4801 acgatgcacg aatgtcgcgg cgaaatatct gggaacagag cggcaataag tcgtcatccc
     4861 atgttttatc cagggcgatc agcagagtgt taatttcctg catggtttca tcgttaaccg
     4921 gagtgatgtc gcgttccggc tgacgttctg cagtgtatgc ggtattttcg acaatgcgct
     4981 cggcttcatc cttgtcatag ataccagcaa atccgaaggc gagacgggca cactgaatca
     5041 tggctttatg acgtaacatc cgtttgggat gcgactgcca cggccccgtg atttctctgc
     5101 cttcgcgggt tttgaatggt tcgcggcggc attcatccat ccactcggta acgcagatcg
     5161 gatgattacg gtccttgcgg taaatccggc atgtgcagga ttcattgtcc tgctcaaagt
     5221 ccatgccatc aaactgctgg ttttcgttga tgatgcggga ccagccatca acgcccacca
     5281 ccggaacgat gccgttctgc ttatcaggga aggcgtaaat ttctttcgtc cacggattaa
     5341 ggccgtactg gttggcgacg atcagcaatg cgatgaactg cgcatcgctg gcatcacctt
     5401 taaatgccgt ctggcgaaga gtggtgatca gttcctgtgg gtcgacagaa tccatgccga
     5461 cacgttcagc cagcttccca gccagcgttg cgagtgctgt actcatccgt tttatacctc
     5521 tgaatcaata tcaacctggt ggtgagcaat ggtttcaacc atgtaccgga tgtgttctgc
     5581 catgcgttcc tgaaactcaa catcgtcatc aaacgcacgg gtaatggctt ttttgctggc
     5641 cccgtggcgt tgcaaatgat cgatgcatag cgattcaaac aggtgctggg gcaggccttt
     5701 ttccatgtcg tctgccagtt ctgcctcttt ctcttcacgg gcgatctgct ggtagtgacg
     5761 cgcccagctc tgagcctcaa gacgatcctg aatgtaataa gcgttcatgg ctgaactcct
     5821 gaaaatggct gtgaaaatat cgcccgcgaa atgccaggct gattaggaaa acaggaaagg
     5881 ggggtagtga atgcttttgc ttgatctcag tttctgtatt aatatccatt ttttataagc
     5941 gtcgacggcc tcacgaaaca tcttttcatc gccaataaaa gtggcgatag tgaatttagt
     6001 ctggatagcc ataattgttt gatccatttt tcgggactcc tggctgatta agtatgtcga
     6061 taaggcgttt ccatccgtca cgtaatttac gggtaattcg ttcaagtaaa gattcggaag
     6121 ggcagccagc aacaggccac cctgcaatgg catattgcat ggtgtgctcc ttatttatac
     6181 ataacgaaaa acgcctcgag tgaagcgtaa ttggtatgcg gtaacgccgc gctcaggcgg
     6241 ctttgatagt catatcatct ggatcaaata ttcctgatgt atcgatatcg gtaattctta
     6301 ttccttcgct accatccatt ggaggccatc cttcctgacc atttccatca ttccagtcga
     6361 actcacacac aacaccatat gcatttaagt cgcttgaaat tgctataagc agagcatgtt
     6421 gcgccagcat gattaataca gcatttaata cagagccgtg tttattgagt cggtattcag
     6481 agtctgacca gaaattatta atctggtgaa gtttttcctc tgtcattaag tcatggtcga
     6541 tttcaatttc tattgatgtt ttccagtcgt aatcaatgat gtattttttg atgtttgaca
     6601 tctgttcata tcctcacaga aaaaaatcgc cctcacatta gagggcaaag aagatttcca
     6661 ataatcagaa caagtcggct cctgtttagt tacgagcgac attgctccgt gtattcactc
     6721 gttggaatga atacacagtg cttactcgta ctaataaaat acccaatttt ctgtttcttg
     6781 gttgtgtcca aagttatatt caatatctgg tgttgatgta tcaatatttt tcatcccatc
     6841 aacaagagtt gatacaacag ccaaatcttg ttttattctc attaaatggt atttcttccg
     6901 gcgcaataaa ctttcaatag caagtttctt cgttgggaat gcaaaagatc tttctgcatt
     6961 ttttgctact ttcttaattg catatctatt tctcctttgt ttccattcct gtaaccactg
     7021 atttggtgct ggtttaaaat taacaatcca atgcgcagga accaaccatg cataatgctc
     7081 tgtctgatga aaagctatat attgaagtgc gaatattttt atcccatctt cttcaactgt
     7141 cgcctggaat ctccagaaaa caggcattcc atcatgttca gtttctgatt caggaaaagg
     7201 tacgctccat gattttgtca tatctcacct caaataagtg gtttgctgcc taatttcatt
     7261 ttctggcgac caacacaagt cacacccatt tcactgcgtg gcttgctgta ataaattagg
     7321 ttagttcagc caataaaaaa cccaccgaag tgggctatga ccatttttta tttggatttc
     7381 gttggtgagc gtgattaaca actctgtgca ttacatcctc atatttttca tcttcaattt
     7441 tttcgacatc gcgaggaaat ggtgttgcta atgctttgtc aactttgtcc attgggtctt
     7501 cattaatctt atattcagga ccgtcatcta tagcattaaa tccaggtgtt acaccgtttt
     7561 ttaatgcata tgctatcctc ttttcccatc tcgctattct cctcctgtct cgagatgtaa
     7621 gacctctatc agatactttt ctgttttgtc cgcggtcagg attaacataa atagtctttt
     7681 tcaccataag catactcaat aagcaccgta cggtagttta ctgtacaatt ttattttttg
     7741 gactgcatgt attttgtttc ctaatgggtt tgaatccttg taataaatac ttctattttt
     7801 tcgaacaact tcttctttct tcttgcagca aaggcttcct agtgatgctg ctttgtctgc
     7861 tctgacgcaa ccagagagct ttagcgcaat ttttcgcgcc agtgcttcat tactgcgtcg
     7921 ctcggcaata agttctgctc tgcgagcttt gtagcggctt tttgccgtac ctttggattc
     7981 tttccagaca atggttacca tgatggtctc ctttaagtgg ctttggcgca tgacgcgtcg
     8041 aggtgcttat cttctcgatc gctgtcttgc agctgcaatt cgcgccatcc ccaaaaccac
     8101 tcaagttctg gtctcaacgg ttaggttgag agttcgtcga tgttaaagag cctgccaatc
     8161 tgttccgttt ggcttccagc ctcctgctga cggcttaaaa ttaagacctc ttaatttttt
     8221 ggtcaagtgc atttttgaag aaaacttaat tttatgggcg cgaatttagt ttgtctttga
     8281 tttttaacgg gaaataaaaa aggggcgaaa gccccttaag gaaggtttgc tagcttggca
     8341 tcaacgacaa cgccaatgat tttacagttc ccattgattt caatcattgg gtattgtgga
     8401 ttgagtggtt tcaggaattt tctaccggca tcaataacta actttttgaa tgtcgcctcg
     8461 ttttctcctt caagtttggc gactaccagc tttccattac gtggttcgac ttctgggtcg
     8521 acgagaataa tcatcccttc aggaatactc agtcctgccg gggcagtcat tgaatcacct
     8581 ttaacgtcga gccaaaaaga gtcttcagaa caatctaccg ttgtgtcgta ccagttatct
     8641 attgcacgcc tatgatatgg ctctacagct tccatccaac atcctgcgct tacccaacta
     8701 attagaggat acgaacctct tggatcatgc ctgctgtgat aggcaatgtt tgaaagacta
     8761 tcctctcctt tcaacaggta atcaggggag cactgcaaag ccttggctaa ggccaatagg
     8821 ttttcgccat tgggctcagt ttcagatcgc tcccattggg aaatagcaac attagacacg
     8881 ccaaccatct tgccaagggc agcctgccta atcttgagtt cttttctgcg agcgcgaata
     8941 cgctcaccca tcagttgtgt attcatagtt aagacatctt aaataaactt gacttaagat
     9001 tcctttggtg gataatttaa gtgttcttta atttcggagc gagtctatgt acaaaaaaga
     9061 tgttattgac cacttcggaa cccagcgtgc tgttgctaaa gcactaggca ttagcgatgc
     9121 agcagtctct cagtggaaag aagttatccc agagaaagac gcctatcgat tggaaatcgt
     9181 tacagctggc gccctgaagt atcaagaaag tgcttaccgc caagcggcat aagtaaattg
     9241 ctctttaaca gttctggcct ttcacctcta accgggtgag caaacatcag cggcaaatcc
     9301 actgggtgtg ccgctatata tcaatatagg aaaattaaca aatggcacaa gcaagttaca
     9361 gcaagctaac acagcgagaa attgatcgcg cagaaacaga tttactcatc aacctgtcaa
     9421 cgcttaccca gcgcggtctg gcaaagatga ttggctgtca tgaatcgaag ataagcagaa
     9481 cggactggag atttattgct tcggtcttgt gtgctttcgg aatggcatca gacatcagtc
     9541 cgattagcag ggcttttaag tatgcgcttg atgaaatcac aaagaaaaaa tccccggtgg
     9601 ccgccgggga ctctaagcaa attgatatgc aattctgagg gaattactgg atcaatccac
     9661 aggagtcatt atgacaaata cagcaaaaat actcaacttc ggcagaggta actttgccga
     9721 acaggagcgt aatgtggcag atctcgatga tggttacgcc agactatcaa atatgctgat
     9781 tgaggc
//
LOCUS       DAZGOB010000160         9785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGOB010000160 DAZGOB010000000
VERSION     DAZGOB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.160"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(22..135)
                     /locus_tag="ACHLIF_004417"
     CDS             complement(22..135)
                     /locus_tag="ACHLIF_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO9608302.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            738..2021
                     /gene="ydfJ"
                     /locus_tag="ACHLIF_004418"
                     /pseudo
     CDS             738..2021
                     /gene="ydfJ"
                     /locus_tag="ACHLIF_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            2110..3570
                     /locus_tag="ACHLIF_004419"
     CDS             2110..3570
                     /locus_tag="ACHLIF_004419"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO9608303.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPAIGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNLIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3606..3809)
                     /gene="ydfZ"
                     /locus_tag="ACHLIF_004420"
     CDS             complement(3606..3809)
                     /gene="ydfZ"
                     /locus_tag="ACHLIF_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO9608304.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3987..4673)
                     /gene="rspR"
                     /locus_tag="ACHLIF_004421"
                     /gene_synonym="ydfH"
     CDS             complement(3987..4673)
                     /gene="rspR"
                     /locus_tag="ACHLIF_004421"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO9608305.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4762..5508)
                     /gene="ydfG"
                     /locus_tag="ACHLIF_004422"
     CDS             complement(4762..5508)
                     /gene="ydfG"
                     /locus_tag="ACHLIF_004422"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO9608306.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5645..7690
                     /gene="dcp"
                     /locus_tag="ACHLIF_004423"
     CDS             5645..7690
                     /gene="dcp"
                     /locus_tag="ACHLIF_004423"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO9608307.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHSVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7735..8253)
                     /locus_tag="ACHLIF_004424"
     CDS             complement(7735..8253)
                     /locus_tag="ACHLIF_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO9608308.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            8529..8921
                     /gene="ydeI"
                     /locus_tag="ACHLIF_004425"
     CDS             8529..8921
                     /gene="ydeI"
                     /locus_tag="ACHLIF_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO9608309.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            9176..>9785
                     /gene="dgcZ"
                     /locus_tag="ACHLIF_004426"
     CDS             9176..>9785
                     /gene="dgcZ"
                     /locus_tag="ACHLIF_004426"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO9608310.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDSFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVY"
BASE COUNT         2524 a         2152 c         2436 g         2673 t
ORIGIN      
        1 tctgattcag gatatgttga cttatacatc ggttttgtct gggttattgg atatgccaat
       61 ccctaatttt attagggcat gactaaaaat gctgaatatg ataaggagag aagtgattat
      121 cagtatgctg ttcatatagc ctcgaattag taatgtgtta tatatgatat agttgacaat
      181 ttttatcttg ggtgttctta aagttcgtag ataaacattg tcgtttcagg tatacaggaa
      241 tgctaacagg tggcagcaaa aatcaggcgg tttatggcgc aagctgaagc ggcaactgca
      301 aactatctta tgcagagact ctacacggat tgggtttaaa agtatacata gataacagtt
      361 tttatctgaa aaagaaaaat atcaaggtga tatagcctat atgcctttga tgtggaggaa
      421 tgaatgtgat gggagtgatg tatctgaata gttgaaaaac cgcagacaca ccttatgcaa
      481 gaacgtgctg cggttggctg gtaaattttt cgatagtgcg agtattgaat gatttccagc
      541 cgttattgat tttacgcgta aatccatgaa aaaactactt atctgttggg gagttttttg
      601 gggcatatat gggacagaaa taggccccag atagacactg agatcgaact taggatgctt
      661 ttaaaaaaat gcaactatct gaaaaaacct agaaaacgcc aaggaaacca caggatggga
      721 aaaaacacct gtgaattatg gatttccagt tatattcgct cggcgcagcg ttagtgtttc
      781 atgaaatatt ttttcctgaa tcatcaacgg caatggcgtt aattttggca atgggaacct
      841 acggtgcagg ttatgtggcg cgtattgtcg gagcatttat tttcggcaaa atgggcgaca
      901 gaatagggcg taaaaaagtg ctctttatta ccatcaccat gatggggatc tgtaccacct
      961 taattggtgt gttaccgacc tatgcacaga ttggtgtttt tgcccccatc ttgctggtga
     1021 cgctgcgtat tattcagggg ttgggtgcag gtgcggaaat ttccggtgcc ggtacgatgc
     1081 tggcggaata tgcgccaaaa ggtaagcgcg gaattatctc ctcatttgta gctatgggaa
     1141 ctaactgcgg aaccttgagc gcaacggcaa tctgggcctt tatgttcttc attctcagta
     1201 aagaggaact gcttgcgtgg ggatggcgta taccgttcct ggcgagcgtt gtcgtgatgg
     1261 tctttgctat ctggttgcgt atgaatctga aagaaagccc ggtctttgag aaggttaacg
     1321 acagcaacca acccacagca aaacctgcac ctgctggtag catgttccag agcaaatcct
     1381 tctggctggc aacagggctg cgttttggtc aggcgggtaa ctccgggtta attcagactt
     1441 tccttgcagg ctatttagtg cagacgttat tgtttaacaa agcaattcca acagatgcat
     1501 tgatgattag ttcgattctc ggctttatga ccattccgtt ccttggttgg ttatccgata
     1561 aaattggtcg ccggatcccg tatattatta tgaatacctc cgcgattgtg ctggcatggc
     1621 caatgctttc tatcattgta gataaaagct atgccccgag caccattatg gttgcactga
     1681 ttgtgattca taactgtgcg gtgctgggat tatttgctct ggaaaacatt accatggcag
     1741 aaatgttcgg ctgtaaaaac cgctttaccc ggatggctat ttctaaagaa attggtggtc
     1801 ttatcgcttc cggttttggt cctatcctgg cgggtatttt ctgcaccatg acggaatcct
     1861 ggtatccgat cgccattatg atcatggcat attcagtaat tggtttaatc tctgcgctga
     1921 aaatgccaga ggtgaaagac cgtgatttaa gtgcgctgga agacgctgcg gaagatcaac
     1981 cgcgtgttgt aagagctgcg caaccttcca gaagtctgta aatcctcaat cccttctctt
     2041 accggagagg ggatttttat tcatataaca aaacatatag cttgccatat ttatatttaa
     2101 ggaattgtta tgggaaataa tttgttatca gcaaaagcga cactccctgt ttatgatcgt
     2161 aataacctgg ccccaagaat tattcattta ggctttggtg catttcaccg tgcgcatcag
     2221 ggcgtgtatg ccgatattct tgctacagaa catttcagtg actggggata ttatgaggtc
     2281 aacttaatcg gcggcgaaca gcaaattgcc gatttacaac agcaagataa tctttatacc
     2341 gttgcggaaa tgtcggccga tgcgtggacg gctcgcgtcg ttggcgtcgt taaaaaagcc
     2401 ttgcacgtac agatggatgg cttagaaacc gtgttggctg cgatgtgtga accgcaaatc
     2461 gcgattgtct ctctgacaat caccgaaaaa gggtatttcc actctccggc gatcggacag
     2521 ttaatgctcg atcacccgat ggtcgctgcc gacgtgcaaa atccccacca gccgaaaaca
     2581 gcaacaggag tgattgttga ggcgctggcc cgccgtaaag cggcaggact tcccgcattt
     2641 accgtcatgt catgtgacaa catgccagaa aacggtcatg ttatgcgtga cgttgtcact
     2701 tcatacgcga aagctgttga tgtaaaactg gcacaatgga tcgaagataa cgtgactttc
     2761 ccatcaacaa tggtggaccg tattgtgccc gcagtgacag aggatacgct ggcgaaaatc
     2821 gaacaactta ccggtgtggg cgatcctgcg ggcgttgcct gtgaaccttt ccgccagtgg
     2881 gtaatagaag ataactttgt tgccggacgt ccagaatggg aaaaagcggg agccgaactg
     2941 gttagtgatg tgctgcctta tgaagagatg aagttgcgca tgctcaacgg cagtcattca
     3001 ttcctggcgt atttgggtta tcttgctgga tatcagcaca ttaatgactg catggaagat
     3061 gaacattatc gttatgcggc gtatggcttg atgttgcagg aacaagcgcc gacgctgaaa
     3121 gtgcagggcg ttgatttgca agattacgct aaccgattaa ttgcacgcta tagcaacccg
     3181 gcgttacgtc atcgaacctg gcagattgcg atggatggta gccagaaatt gccacagcgg
     3241 atgttggatt ctgttcgctg gcatctggcg catgacagca agttcgatct gctggcgctg
     3301 ggcgtcgcgg gttggatgcg ttatgtcggt ggtgttgatg aacagggaaa tctgatagaa
     3361 atcagtgacc cactgttacc tgttattcag aaggctgtac aaagtagtgc cgaagggaaa
     3421 gcgcgcgtcc agtcattgct ggcgattaag gcaatctttg gtgatgattt gccagacaat
     3481 agcttgttta ctgcaaaagt gacggaagcg tacttgtctt tattagcgca tggcgcgaaa
     3541 gcgaccgtgg cgaaatattc cgtgaagtaa aaaaagcaat gccgccatcg gcggcattca
     3601 cacacttagt tcatgccgag acgaatcagg tccagaggtg ccagtttctc ttcacaacct
     3661 tcaacaacta cggtttttcc gcggcggatt tgctcagcgg tcagaccttc agtatcaatc
     3721 gacaggatct tgccagtgtg accggtgccg ctaaccataa cacggctgcc agtggtgatt
     3781 gcgttacggt tacgatcgta ggtcgtcata ggtgttttct cctttctgat ttacagtcga
     3841 agcccacgta acgggcgcaa tataaatact cctgtcgagt gaaatatttt ttgtttttga
     3901 tcaagctcac atcttttttg atgcatgcat gagccgtaag gcggggggaa aagcctctca
     3961 tcccggatgg gatgagagta aatgaattac tcttcgctaa accagtcgct gttttcctgg
     4021 cggatctggc gcacggattc gctgatttcc tgcaaatgtt gcgtcattgc acgttctacc
     4081 gcatcgccat cacgtttttg cagggcagag aaaatatcaa gatgctggcg taacagcatt
     4141 tctggtggag aaacatggtc gaaactcata tagcgcacgc gatcaacggt cgctttcagg
     4201 ttctcaatgg tatcccacgc cagttgacag tcggcaatct gcgtcaggag ttgatggaag
     4261 ttgtcatcaa gttcaaaaaa atcatccagt tgcttgcgct caatggcaat gcgttgctgg
     4321 tgaagatttt gttccagttg gtagcactgg ctttcggtaa tcatgctcgc cgcccgacgc
     4381 gccaccgcgc actcaatggc ctgacggata aaactgccgt ttcgcacctg ggccatggaa
     4441 attttgttga cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt ttccgccagt
     4501 ttaataaagg cttcacgaac cggctggcgt gacacattga aacgaacaga aacttctttt
     4561 tccgacaacg gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg aagaatacga
     4621 taaatctgct gattgacagg ctgtgtggga ttaagttgcg tttcgacggt cattcgttct
     4681 tgcttgtgag tgagttaact gcatgagtct accacttttt tgtgacaagc aataacccgg
     4741 cagttacgcc gggtataaaa attactgacg gtggacattc agtccggcat agctttgggt
     4801 aaccggcatc atttccaggg tattgatatt gacgtgagca ggcagcgttg acacccacca
     4861 gacggcttcg ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg ttttttccgc
     4921 tttaccgtca tcgcctttaa agcggacatt ggaaaactcg gtgccaccca ccagacccgg
     4981 ttcgatgtcg gtgacgcgca ccgccgtacc atgcaggtcc gtacgcagat tcaggctaaa
     5041 ctgacgaaca aacgctttcg tcgcaccgta aacgttacca ccggcataag gccagctacc
     5101 tgccgttgag ccaatgttaa taatatgacc atgattacgt tcaaccatac ccggtaagac
     5161 ggcgcgcgtc atatatacca ggcctttgtt gttggtatca atcatcgttt cccagtcttc
     5221 aacgctggct ttatgcgcag gctccatgcc caacgccagg ccggcattat ttaccaggat
     5281 atcaatattg caccactcgg cagggagcga tgccagcatc tcttcaatag cggcgcggtt
     5341 gcgaacgtcc agttgggcga tatacagatt atctcccagt tcgtctttta actcctgcaa
     5401 ccgctcctgg cgacggccag tggcgataac tttatgccct tgttgaataa aacgacgagt
     5461 aatgcattca ccaaaacctg ccgttgctcc agttactaaa acgatcatct cactgttcct
     5521 caacgctttt gtgtgtcaat aacatagcac gtgatgctta agagcgggta atactggttt
     5581 gcaagattgc actccgttat cagtaagcct acgctatgga acacctcgcg ttcaggagaa
     5641 gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc tggctcctca
     5701 ttttgatcaa attgccaatc atcactatcg cccggcattc gatgagggaa tgcagcaaaa
     5761 gcgggcagaa attgctgcca tcgcgcttaa cccgcaaacg cctgatttca acaatactat
     5821 tctggcactg gaacaaagcg gagaattact tacccgcgtt accagcgtct tttttgcgat
     5881 gactgcggcg cataccaatg atgaattaca gcgtcttgat gaacagtttt ccgctgaact
     5941 ggcggaactg gctaatgata tctatctgaa cggtgaatta ttcgcgcggg tagatgctgt
     6001 ctggcagcgc cgtgaatccc tggggcttga tagtgaatcc atccgcctgg tggaggtgat
     6061 tcatcaacgt tttgtccttg ccggagccaa acttgcgcaa gctgataaag caaaattaaa
     6121 agtactgaat acagaagctg cgacactgac cagtcagttt aaccagcggt tactggcagc
     6181 aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa tgagtgagca
     6241 agagattgcg ctggcggcag aggcggctcg cgagaaaggt ctggataaca aatggctgat
     6301 tccgctgctg aataccaccc aacaaccggc gcttgccgaa atgcgcgatc gtgcgacgcg
     6361 tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa aatgatggca atgatacccg
     6421 cgctatcatt caacgtctgg tggagatccg tgcacaacag gcgacattac ttggttttcc
     6481 tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa acacctgaag cagcgctcaa
     6541 ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgcg agcgatgaat tagcctccat
     6601 acaggcggtt attgataagc agcagggcgg gtttagcgcg cagccgtggg actgggcatt
     6661 ttatgccgaa caggtacggc gggagaaatt tgatcttgat gaggcgcagc tcaagccata
     6721 ttttgaatta aacacggtgt tgaatgaagg tgtattctgg accgcgaatc agctcttcgg
     6781 tattaagttt gtcgaacgtt ttgatattcc tgtctaccat cctgacgttc gtgtgtggga
     6841 aatttttgat cataatggcg tgggactggc gttattttac ggtgatttct tcgcccgtga
     6901 ttcaaaaagc ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc ttaatgaaac
     6961 gcattcggta atttataacg tttgcaatta tcagaaaccc gctgccggtg agcctgcgtt
     7021 gttactctgg gatgatgtca taaccttatt ccatgaattt ggtcatacgc tgcacggcct
     7081 ttttgcccgc cagcgttatg ccacgctttc cggcaccaac acgccgcgtg attttgtcga
     7141 atttccgtcg caaatcaacg aacactgggc aacgcatccg caggtattcg ctcgctacgc
     7201 ccggcattat cagagcgggg cagcaatgcc tgacgaactg caacagaaaa tgcgtaatgc
     7261 cagcctgttc aacaaagggt atgagatgag cgaactgctt agcgccgcac ttctcgatat
     7321 gcgctggcat tgcctggaag aaaacgaagc aatgcaggat gtcgatgatt ttgaattgcg
     7381 ggcgctggtg gcggaaaata tggatcttcc tgctataccg ccacgctatc gcagcagtta
     7441 tttcgcccat atttttggtg gcggatatgc cgcaggttat tacgcttatc tgtggacaca
     7501 aatgttggcc gatgatggtt atcagtggtt tgttgagcag ggcggattaa cgcgtgaaaa
     7561 tgggctgcgt tttcgcgagg cgatcctttc cagaggtaac agcgaagatc tggaacgcct
     7621 gtatcgacaa tggcgcggta aggctcctca gattatgccg atgctgcaac atcgtggctt
     7681 gaacatataa acaccaccaa tggctgtcct ggagatatcg ccaggccagc ctggttatag
     7741 cagtaattgc agcagctggg cgagggcaaa cctcaccgct aaagctaata ccgtttcgca
     7801 gtcgccagca aaatgcataa ccgcagtata ggtctggcct ttaatatgcc acgcgaacca
     7861 gacggtaccc gctggcgtac catcttcacc gccctccggt ccgccgtagc cggtaatggc
     7921 aatacttaca tcagcatccg cacgctctat ggctccggtt gccatttctg ctgccacttt
     7981 ctcactcacc gcagaatatc gttcaagaga ttgctggctt acgctgagga ttttcatctt
     8041 tgcctgatcg gtgaaagtaa caaagcctgc accgtaaaat ttgggtgtat cttcagctgc
     8101 acacagggcg ctagccaact taccgccggt gcatgattcg gcggtagtta agcgtaattg
     8161 atccgcgata agtctttgac tcaacgcgga tgtcaggttt tcaatagtgt cggtatctgc
     8221 gagttgtaca attttatctt tattaatgtt cactgttttt tgtccacgct attatatgta
     8281 aaggtgattt gctttttctg tatgctcgtc aaatataata attttcaaac atataaatcc
     8341 gattattctg aaaatatgtt taagactctt caatcttctt aactttagtg agttaaattc
     8401 tctgaatatt tgacagctag tgggctgatt ttaaatgcct tataacaatt ggcagtattc
     8461 ttatttggca attataaaaa tagcaaccag acttaataag tcaaaccaaa cgcaattaag
     8521 gagttagtat gaaatttcag gccattgtat tagcaagttt tcttgtcatg ccttatgcgt
     8581 tggcagatga ccagggcggt ttaaaacagg atgcagcgcc accgccgcct catgcaatag
     8641 aagatggcta tcgcggaacc gatgatgcaa aaaaaatgac cgttgatttc gcaaaaaaca
     8701 tgcacgatgg cgcttcggtt tcactacgcg gtaatttgat ttctcacaaa ggagaggacc
     8761 gttacgtttt tcgcgataag agcggtgaaa ttaatgtcgt tattcctgcg gccgtctttg
     8821 atggacgaga agttcagccg gaccagatga tcaacattag cggcagtctg gataagaaaa
     8881 gcgcgccggc cgtcgttcgg gtcactcatt tgcagaaata aaaataaatt agcctgatgg
     8941 cctgaactac tgggccatta gggtattggg ttatgctatt tgtcatcctt ttatttacga
     9001 taatgcagag gtgatttaca gaattaaaca cttttttatc cgctgatttt gttttaaatc
     9061 acaatgttat ctcagagatg actcataact tcggatttct atgaaaatat tgacgtaatt
     9121 ttgcttttct ctcgttagaa tagcgcgcac aaggaactgt gaaaaaggag tggcaatgat
     9181 caagaaaaca acggaaattg atgccatctt gttaaatctc aataaggcta tcgatgccca
     9241 ctaccagtgg ctggtgagta tgtttcacag cgtggtcgcg agagatgcca gtaagccaga
     9301 aataacggat aaccattctt atggactgtg ccagtttggt cggtggattg atcatctggg
     9361 gccactcgat aacgatgaat taccttacgt tcggctaatg gattctgccc atcaacatat
     9421 gcataactgt ggtcgggaat taatgctggc tattgttgaa aatcactggc aggacgcgca
     9481 tttcgactcc tttcaggagg ggttgctttc ttttactgcg gcattaaccg attacaaaat
     9541 ttatttgctg acgatccgta gcaatatgga tgttttgacg ggattgccgg ggcgtcgggt
     9601 tcttgatgaa tcctttgatc atcagttacg caacactgag cctctgaatc tttatttaat
     9661 gttgttggat attgaccgat ttaaattggt taatgatacc tacgggcatt taatcggcga
     9721 tgtagtatta cgcaccctgg caacttactt agccagttgg acgcgtgatt acgaaacggt
     9781 ttatc
//
LOCUS       DAZGOB010000161         9609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGOB010000161 DAZGOB010000000
VERSION     DAZGOB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.161"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            142..1206
                     /gene="ulaG"
                     /locus_tag="ACHLIF_004427"
     CDS             142..1206
                     /gene="ulaG"
                     /locus_tag="ACHLIF_004427"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO9608311.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1314..2069
                     /gene="ulaR"
                     /locus_tag="ACHLIF_004428"
     CDS             1314..2069
                     /gene="ulaR"
                     /locus_tag="ACHLIF_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO9608312.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2066..2815)
                     /gene="yjfP"
                     /locus_tag="ACHLIF_004429"
     CDS             complement(2066..2815)
                     /gene="yjfP"
                     /locus_tag="ACHLIF_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9608313.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITTRHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2997..3326
                     /gene="bsmA"
                     /locus_tag="ACHLIF_004430"
     CDS             2997..3326
                     /gene="bsmA"
                     /locus_tag="ACHLIF_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO9608314.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3475..3750
                     /gene="yjfN"
                     /locus_tag="ACHLIF_004431"
     CDS             3475..3750
                     /gene="yjfN"
                     /locus_tag="ACHLIF_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO9608315.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3867..5492)
                     /locus_tag="ACHLIF_004432"
     CDS             complement(3867..5492)
                     /locus_tag="ACHLIF_004432"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO9608316.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5576..6739)
                     /locus_tag="ACHLIF_004433"
     CDS             complement(5576..6739)
                     /locus_tag="ACHLIF_004433"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO9608317.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6742..7380)
                     /locus_tag="ACHLIF_004434"
     CDS             complement(6742..7380)
                     /locus_tag="ACHLIF_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO9608318.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7390..7788)
                     /locus_tag="ACHLIF_004435"
     CDS             complement(7390..7788)
                     /locus_tag="ACHLIF_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO9608319.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7806..8465)
                     /locus_tag="ACHLIF_004436"
     CDS             complement(7806..8465)
                     /locus_tag="ACHLIF_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO9608320.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8516..9214)
                     /locus_tag="ACHLIF_004437"
     CDS             complement(8516..9214)
                     /locus_tag="ACHLIF_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO9608321.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9233..>9609)
                     /locus_tag="ACHLIF_004438"
     CDS             complement(9233..>9609)
                     /locus_tag="ACHLIF_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjfI family protein"
                     /protein_id="HFO9608322.1"
                     /translation="TALQTVPEQNIDVTNSESALIIKMNDYGDLQINILFTSRQMIIE
                     TFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDIL
                     LEITSLVDNALDLAEITEEYSH"
BASE COUNT         2502 a         2525 c         2285 g         2297 t
ORIGIN      
        1 ctttccacac tcaaaatcaa caacaacttc cctggcaaaa agtcaaaaaa tggtcattgc
       61 cttcctgaaa aaatagtgtc aggattaatc aaaaccaatc acatattgat tcgaattgaa
      121 ctatcatgaa ggtaaatggc gatgagtaaa gtgaaaagta tcacccgaga atcctggatc
      181 ctgagcactt tcccggagtg gggtagctgg ctgaatgaag aaattgaaca agagcaggtc
      241 gcccctggca catttgcgat gtggtggcta ggctgcaccg ggatctggtt gaaatcagaa
      301 ggtggcacca acgtttgcgt tgatttctgg tgcggcactg gcaaacaaag tcacggtaac
      361 ccgttaatga aacagggtca ccagatgcag cgcatggctg gcgtgaaaaa actgcagcca
      421 aacctacgca ccaccccgtt tgttcttgat ccgtttgcga ttcgccagat cgacgcggta
      481 ctggcgactc acgatcacaa cgatcatatc gacgttaacg tcgctgctgc cgtgatgcag
      541 aactgtgctg atgacgtacc attcattgga ccgaaaacct gcgttgattt gtggattggc
      601 tggggcgtac cgaaagagcg ttgcatcgtg gtcaaaccgg gcgatgttgt aaaagtaaaa
      661 gacattgaaa ttcatgcgct tgacgctttc gaccgtactg cactgatcac cctgcctgcc
      721 gatcaaaaag cggctggcgt actgccggac ggcatggacg atcgcgcggt gaactatctg
      781 ttcaaaacgc ctggcggctc cctgtatcac agcggcgact cccactactc taactattat
      841 gcgaagcacg gtaacgaaca tcagatcgat gtggcgttag gatcgtatgg tgagaacccg
      901 cgcggtatta ccgacaaaat gaccagcgcc gatatgctgc gtatgggtga agccctgaat
      961 gcgaaagtgg ttatcccgtt ccaccacgac atctggtcaa acttccaggc cgatccgcaa
     1021 gagatccgcg tgctgtggga gatgaaaaaa gatcgtctga agtatggctt caagccgttt
     1081 atctggcagg ttggcggcaa atttacctgg ccgctggata aagacaactt cgagtaccac
     1141 tatccgcgcg gtttcgatga ttgcttcact attgaaccgg atctgccgtt caagtcattc
     1201 ctgtaatctg tcgtttatac cggatagcgt ggagaagttc gcgctatccg gcgtagtatt
     1261 tcctggctat ttcaaatatc atctaaaaaa atcaaatttt atcggaatag ctcatgactg
     1321 aagcacaaag acatcaaatc ctcctggaaa tgctcgcaca attgggcttt gtgaccgttg
     1381 agaaagtcgt tgagcgtctg ggaatttcgc ctgccactgc gcgacgcgat atcaataaac
     1441 ttgacgaaag cggcaaactg aaaaaagtgc gcaatggcgc agaagctatt acccagcagc
     1501 gcccgcgctg gacgccgatg aatctgcatc aggcgcagaa tcacgatgaa aaagtacgta
     1561 tcgctaaagc agcctcgcag ctggttaatc cgggcgaaag cgtagtcatc aactgcggct
     1621 ccaccgcgtt tctgcttggg cgggaaatgt gtggcaagcc agtgcaaatc atcactaatt
     1681 atctaccgct ggcaaattac ctgatcgatc aagaacatga cagcgtgatc attatgggtg
     1741 gacagtacaa caaaagtcag tccatcactt taagcccgca gggcagcgaa aacagtctct
     1801 atgccgggca ctggatgttt accagcggaa aagggctgac cgcagaaggg ttgtataaaa
     1861 ccgatatgct gacagcaatg gcagagcaga agatgctgag cgtggtaggg aaactggtgg
     1921 tactggttga tagcagtaag attggcgaac gcgcgggaat gctttttagc cgtgccgatc
     1981 aaatcgatat gctgatcacc ggcaaaaatg ctaacccgga aatcctgcaa caactggaag
     2041 cgcaaggggt cagcattctg cgtgtttaaa gatgctggcg gaaaaatgtc acggcagcat
     2101 ctaacgcctc aggcgtaatg cggtgacgca cgcctggctg ccatgaacag gttagcagtt
     2161 tatcccgtcc cgtctcgctt aaggcctgct gcaaacgtag tgattcgtcg gcaggcacaa
     2221 cgtcatcgtc gaggccatgc cacagcagta gaggtctgtc cccaagttgt tccaggtggt
     2281 ttgtcgcttc ccactctgcc agcggcgcga taatgttatt gaattcattc tgctgtgctg
     2341 ccgtttcagg tatcagcggt ggaaacagtg aacgggcgag cgatgtaaaa tagcccgatc
     2401 ccatcatgct ggcggtacat ttcaccgtgg ggtggcgagt ggtaattccc agtgccgtca
     2461 tcgcgcccat cgacgcgcca ccgactgcca gacggtcatc aagcagccag ttttcttcgg
     2521 ctattgccgc acgtaaagta gtgaattcct gcatactttg tagcaagatt tgccagaatt
     2581 gatttaaccg ccgcgcttcg tcgctactaa aacggctacc gtgatcgggc gcatccggca
     2641 tgatcacccg caaaccagcc tgcgccagcg caacggcaaa atagctatac accaggctgg
     2701 atgaagtaaa gccgtgataa aaaattacgc acggtaacgg ggtatctttt tgcccgacag
     2761 gataagcatg aagaacggga atatctgcca gctcgcgtga ttctatttca atcatctgtc
     2821 tctccttttt gtctggtatg acatgaagaa atccttagca caagtttctg ctggcggtat
     2881 tagttcataa tgttgagatg tgggttacgc tttcgtcacg ttttcattcg aaaatcgcgt
     2941 accaggtaac aattcgggaa cattccccca aagttaaatt aataaggcac tacactatgg
     3001 ttagcaggaa acgtaatagc gttatttacc ggtttgccag tttattattg gtgttgatgt
     3061 taagtgcctg tagcgctctg caaggtacgc cacagccagc accgccagta acgaaccatc
     3121 cgcaagagat tcgccgcgac cagacgcaag ggttacagcg aataggtagc gtaagcacga
     3181 tggttcgggg ttctccggat gacgcattag cagaaatcag agcaaaagct gtcgctgcaa
     3241 aagctgatta ttacgttgtc gtcatggtgg acgaaaccat cgtgacagga cagtggtatt
     3301 cacaagccat tttgtaccgt aaataaatca gacaacttac atagtcagct ttacattgct
     3361 ttgcgtccct atgcgtttac ctgtgcacaa taaattaccg gctggaagcc tgggcgttta
     3421 ttaccgcgat ggaatgccct gcaacatgtg gggaaacgaa aaatggagct gacgatgaaa
     3481 caattacttg cctcaccctc gctgcaatta gtgacttatc ctgcgagcgc cacggcacag
     3541 tctgcagaat tcgccagtgc tgactgcgtg acgggcctga atgaaatagg tcagatctca
     3601 gttagcaata tttcagggga tccgcaggac gtggaacgta tcgtggcgtt aaaggccgat
     3661 gaacaaggtg cgtcatggta ccgcattatt accatgtacg aagaccagca gcctgataac
     3721 tggcgcgtac aggcgatact gtatgcatga gtattatata cagcaaatga acaatgccgg
     3781 atgcggcgcg agcgccttgt ccggcctaca atgttcgcat atttcattga gttaatcgaa
     3841 accagcgtcg catcagtcgt atacgcttac acacacactc cccccgtcgc ccgcagcaat
     3901 aaatcattct ggatctgctc tgacaaccgt acgccgccgc gcgtatctaa catcacctga
     3961 caccacgcct gcgccattgg cggagaggca tatttcaaca tttgcgcacc gcagcccagc
     4021 aggaatagct gatgagtaat ctctcgcccc agttcttcag ctggcttgcg cagctgctgc
     4081 tgtaaacgac gaaccgcgcg atcaaaatag cgatcctgcc ctttcacttc cacaaacgct
     4141 tccgacaata agtcgtatac gcccgcttgc ttattgagaa cgcgcagcac atccaggcac
     4201 ataatattgc cggaaccttc ccaaatactg tttaccggca tctcccggta aagccgcggc
     4261 agctcgctct cctcgcaata accaatgccg cccagcacct ccatcgcttc ggccacaaac
     4321 ggcatacctc ttttgcagat cacaaatttt gccgcaggcg taaataaacg cgcccacagg
     4381 gcttctttgg catcggcacg ccggtcccac gctcgcgcaa gacgaaacag caacgccgtt
     4441 tgcccttcaa gctgaagtgc catgcgactt aagacatgac gcataagggg ctgttggatc
     4501 aatggattac caaaaacatg gcgttgatgt gcatgataaa tcgccagcga aaatgcacgg
     4561 cgcatcatgg catggctacc cagggcgcaa tcaaaacgcg tcatcccacc cattttcagg
     4621 atcagacgaa ttccttcccc ttcctgcccc aacaaccagc caatggcatc ctgaaactcc
     4681 acttcgcagc tggcgttaga gcgattaccc agcttatctt tcagccgctc gaggcgaatc
     4741 gcgttgcgtt gcccgtcagg caaaaagcgc ggcacaaaaa agcaggacaa accgcccgta
     4801 gtctgcgcca gcaccagatg cgcatcgctt tgcggcaccg agaaaaacca tttatgcccc
     4861 accagccgat aagagccatc ttccaggcgc tctgcgcggg tggtgttgct catgacatcg
     4921 gaaccgccct gcttttccgt cattcccatg ccaatcaaca aaccgcgttt ttgcccacct
     4981 ggcaataagt gagaatcgta gcgatcgctc agcagcggcg tggtccagtc ctgaaacggc
     5041 gtgggtaaca tctgtaacaa caatggcgtg gcggcaaagg tcatcgttat tggacataac
     5101 gacccagcct caacctgcgc atgtaacata aaacgcgccg cgcgcgccac aaatgcgccg
     5161 gagcgagcgt cttcttccca ggcaagattg tgcacccgat tggtacataa cgcctgcatc
     5221 agcaggtgcc aggcggggtg aaaacgcaca tcgtccagac ggcgtccttg cgcatcgtag
     5281 cgcaataatt caggcggatt cacattcgcc agccgcccca gttcaagaga ttcagccgtt
     5341 cctaactgct gaccaatact ggctagaaaa tcgctatccc agccagcacc ttcacgcgtt
     5401 accgcttcac agagcgcgcc atcagacagg tataagttgc tgttatttaa tggtataggt
     5461 tgattaaaaa cggtgtgagt ttgccagtgc actgtgtctc cctccatcaa tggcagtcac
     5521 ccttagtatg gtcactgcca tggattcctg acagaaagag attgctaaaa cattcttatc
     5581 cagcaatgta atgcggaacg aaacgtgagg tgtctttggt gatcagcgtg ttatcttcac
     5641 ggatccccat tccgcacgct tcatcatcga caatccagct accgatgagt gtgtagctat
     5701 cgccaaatcg cggcagaggt tgaaacgcct ggtagatcat cggttcatcg gcgtaatcac
     5761 catcagcgtg gtcaacgacg ttattctgac cgtcgaaaat ggtgacgttt ccgccttcgc
     5821 gcgagtagat cggtttacgc acatagcttt cgccagcggc aatctgcggt ttttcgccct
     5881 caaaccagga tgcaagaagg ttaggatgac cagggaagaa gcgccaaagc agcggcatta
     5941 gccctttatt actcaagata cttttccata acggctccac ccattgctcg cgacgcttgc
     6001 gcagcagcgg accgttatcg tcacgcatca tccactccag cggatacagc ttaaatgcac
     6061 gctggatgac attatcatca agatcggtca gtacaccgcc gacgcccaaa ccgagatctt
     6121 caatgtagat aaaccgcgac tcctgccctg cctgctgggc gcagtcctgc aagtacagca
     6181 cggtagtcct gtcttcatcg gtgtcctgac agcagcagaa ataaaacggc tcccggctat
     6241 aaagctcgct aaagcgtgaa atcaggcgtt cctgaatggc attgtactga tcggcatcac
     6301 gcggaataat gccgctgcgc cgggcatcct ccagccacaa ccactggaaa taagccgact
     6361 catacaacga ggtcggcgta tcggcgttgt actccagcag cttcaccggc gcattgccac
     6421 accaggcaaa atccatccgg ccatacagcg aaggatcgcg tgcgcgccag ctttcggcga
     6481 tcacatccca gtacaacggc gggatcgcca gttgcgtcag gatctcttcg tctttaaccg
     6541 cgcgatcaac cacctcaagg cacatctgat gcagttccgc agtcggtttt tcgatctgct
     6601 cttcaatctg gcgcagagta aaacggtaag cccgactctc gtcccaatag atttcattgt
     6661 cgatgatatg aaagtcgaaa ccgttgttag cggcgatctg gtccagatcc cgtcgcacag
     6721 gaacgttgtg tctcagcatg attagcctcc ccaatgccca cgggcgctgg aagaacgacc
     6781 atagccgccg cgcgaaacgg ttgacgcttt tttggtggta aagccgcctg aagagtaaga
     6841 ctcttttttg ccagaaccgg agcgccagga gtaatcaccg gaagtgttgc gccagacggg
     6901 gcgggaagca aatgaggatc cgccgctgtt atagacgaac ggctcatcgc gatctttacg
     6961 aataacccgg cttaacaaaa atccagaaac gaccgggatc caactctgtt caacattgtc
     7021 gtagtagcaa ttttcgtact tagactgaca attctgctga gtcatattct tcggaacatc
     7081 ggcataaaat gccgctttgg cgttgttcca gccacgcgcg caaatgtcgg cattattacc
     7141 gtcatcaata caatcctgca ctgtcgaata aaacgttccg tcgccgtcgt tatcgacatc
     7201 gccactatcg ccacaacctt tcaatacaaa aaaagcggca ccgcccatta gagccaacgt
     7261 taagtatttt tgtgcgtagc gattgcgacg cggttcaaaa ggattattcg gtctaccaat
     7321 gcgactgatc gctccgtgac caattttact gttatttctt gatttgcgtt ttctggccat
     7381 gatcgtccat taccatgtca tacaagctgc gttaaaaata ccgccagcca gcgcggcggt
     7441 tcccataaac attcctgctg cggtattgtg attaataatt ttttcgctta atgcgggcat
     7501 ataaagtctg acaccagcaa aaacgagtaa ctgaatcacc agtgcaatcc cgccccaggc
     7561 gaaatagtct ggaatactca ccgcattgat tgccgcactg gataatggga taacgtaacc
     7621 taacaatgta ccactgaacg ccagtgaagc cgcggtatta ttgtttttga ttaactgcca
     7681 ttcgttgtgc ggtgtaattt tagaatagat aaaaagaaaa ataatcacca tagccacgcc
     7741 aataaaaaaa taggcgctaa aggcaagaag tgaatccagt atatgcattg taaacgtcct
     7801 tgtaattaac caataatatg aagtgatgtc agcgggatat cgacacccaa cgcgcgcgaa
     7861 aataaccact ctggctcgcc gagatcgtta aaagattcct caccatttaa cagcaaatat
     7921 tcgtaagtat cttcggttac ttgccgctgg tagcccatgg taaaattatg gacctcccat
     7981 ttggcatggt tttgattttc tactttttcc agcatgtaga cgggttcgat atttcccggt
     8041 tcttcgctgt taaaaaatcg ctgccagcgt ttttcctgcc agtttaagat cattgccccc
     8101 atcactttgg cgttgatcgc ctcgcgccag tgactctctt tactgatacc gtagctctct
     8161 tcatagacaa agagcttgat atcatcaatg tcatcaatat cttcgccgcc ggtggtattg
     8221 atttgcagaa attcatcgcc tgaagtgtag tagcggaaaa tctgactacc gccgcccaga
     8281 tcgatgtggc tgacagcggc taccgtaaat tcctcacccg gcagcgcaat cagcaattca
     8341 tcttccagca aacgaaacgc taacgtgtcg agcgtaaagc cactattgag atgaagtccc
     8401 agcggaccgc gagcgatagc aggcttatta tccttgccaa acagacgctg gaaaaaacca
     8461 gacatatttt atccttaaaa tagttgccag ccttttagcg aggctggcaa aaaaattact
     8521 cgccctgttg gcgttgcagt cttgctaata catcctgggc gctactttta ttgctaccgc
     8581 caattccggc ttccgccagc ttttcatcaa gatcgcgacc gtctgcgact ttttccaact
     8641 gtgcagcagc atccaggcga gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg
     8701 attctgccgc tgtcgaaacg ctggaagatg cgccaacggt agaggttgtt acagcctgtt
     8761 gtgcacgctg catggcttca gtggctttaa cgacttccat ttgctgctca aactgagcga
     8821 tacgctgcgc tgtcgctgta actgcttttt ccacgccatc acgggagact tccagattcg
     8881 acaacacttg ctcttcagcg gtaatgagat tctcaaggcg cgcgatttct tcagcaactt
     8941 cgttaatcaa cgacggatta acgttcttgc ttaacgcttc cagcgcacgg gcttccagac
     9001 tggctttgcg ctcacgtaaa tctttcagct tatcgtgact taatttcacc cgcgccagca
     9061 gatcaacgcg agattttccg gctttatcga gttcagcttt agcgtcacga atatgctgtt
     9121 ccagcatgcg cacgccctgg gtttcttcaa tggattcttc cgcctgggag ataaacgatt
     9181 tccccagcgt aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagtgtg
     9241 aatattcttc tgtaatttca gccagatcca atgcgttatc taccagcgag gttatctcaa
     9301 gcaggatatc ttcaagagaa gatttaagcg ataacgcccc gaaaacaatg taatactctt
     9361 cctgttgcac gctggagatt cctaccgatg acaacggcat catcttctga tttcttaata
     9421 agaaggtatt aaattcatct ggattgctga tactacttac cggacaaata aaggtttcga
     9481 taatcatttg gcgggaagta aaaagaatat tgatttgcaa atcgccataa tcattcattt
     9541 taataattaa tgcgctctcg ctatttgtca catcaatatt ttgttcaggt acagtttgca
     9601 gcgccgtcg
//
LOCUS       DAZGOB010000162         9352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGOB010000162 DAZGOB010000000
VERSION     DAZGOB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.162"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..792
                     /locus_tag="ACHLIF_004439"
                     /pseudo
     CDS             1..792
                     /locus_tag="ACHLIF_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
     gene            1128..2105
                     /gene="glaH"
                     /locus_tag="ACHLIF_004440"
     CDS             1128..2105
                     /gene="glaH"
                     /locus_tag="ACHLIF_004440"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO9608323.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2125..3393
                     /gene="lhgO"
                     /locus_tag="ACHLIF_004441"
     CDS             2125..3393
                     /gene="lhgO"
                     /locus_tag="ACHLIF_004441"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO9608324.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            3416..4864
                     /gene="gabD"
                     /locus_tag="ACHLIF_004442"
     CDS             3416..4864
                     /gene="gabD"
                     /locus_tag="ACHLIF_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9608325.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4878..6158
                     /gene="gabT"
                     /locus_tag="ACHLIF_004443"
     CDS             4878..6158
                     /gene="gabT"
                     /locus_tag="ACHLIF_004443"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO9608326.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            6469..7869
                     /gene="gabP"
                     /locus_tag="ACHLIF_004444"
     CDS             6469..7869
                     /gene="gabP"
                     /locus_tag="ACHLIF_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO9608327.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7890..8552
                     /gene="csiR"
                     /locus_tag="ACHLIF_004445"
     CDS             7890..8552
                     /gene="csiR"
                     /locus_tag="ACHLIF_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO9608328.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(8553..9002)
                     /gene="kbp"
                     /locus_tag="ACHLIF_004446"
     CDS             complement(8553..9002)
                     /gene="kbp"
                     /locus_tag="ACHLIF_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO9608329.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9086..9244)
                     /gene="yqaE"
                     /locus_tag="ACHLIF_004447"
     CDS             complement(9086..9244)
                     /gene="yqaE"
                     /locus_tag="ACHLIF_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO9608330.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2193 a         2385 c         2533 g         2241 t
ORIGIN      
        1 ttggatgaac aacaaatgtc atcactgctt tatgaaagag atgatttaag cgccattgat
       61 ttttcattgc tcatgaaaac gataaaagcc ttttcatttg gtggagatct tcaaaccctt
      121 gcatcaaaac cgggttccac aatctcaagt atcccatcag aaagacggat attgattaac
      181 attaaccacg attttcctaa caatggtaat cttttcaatg actttctatt taaccatcaa
      241 caagatgaac aattagcaat ggcatatata gccgctctgc cgttcagcag gcctttagtt
      301 tactgggatg gccaagtatt aaaatcaacg actgaaatta aaaattatga tgggtccacg
      361 cgtgtcggcg gtgaggcgta gcttaataaa ggttgctcta cctatcagca gctctacaat
      421 gaattccacg cattatatat agataaagca ggaatatgga gcgcatttga gggtgtatct
      481 gcaactaaaa acgttctggc ctttagtcgt ggggattctg tgaacattaa tcactctcct
      541 catgatggac tagttataat aaataaagga aacgaagaag ttgaaggtac ctggcctaac
      601 aaattgcaac ctggaatata caaaaacatg gggagtaata gcgttaacat tattattaat
      661 aatacccgaa aaattatccc ccctggtaaa gtatttacgc ttagaggcgg aactctaaat
      721 atcaatattc ctgggcgtag cgctcttctt ttagggaaaa caggagaacc gccgaactat
      781 ctctatttat aatttaatgt tatatctgcc ccgataaaac ggggcagata atatgtttag
      841 tttactaacg gtcattttgc agtgaagcca tttactgttt tttatcgacc agataatctg
      901 ttctctaatg ttaactcccc ctaacctgtt gctttagtta ttcatttcct gtctcacttt
      961 gccttaatac cctacgttaa atgttactaa tttgttgctt ttgatcacaa taagaaaaca
     1021 atatgtcgct tttgtgcgca tttttcagaa atgtagatat ttttagatta tggctacgaa
     1081 atgagcatcg ccatgtcacc ctacatctca taagaggatc gcttctgatg aatgcactga
     1141 ccgccgtaca caataacgct gtcgattcag gccaggacta tagcggattc actctcatcc
     1201 cgtcggcgca atccccgcgt ctgctggaac tcaccttcac cgaacagacg accaaacagt
     1261 ttctcgaaca ggttgccgaa tggcccgtgc aggcgctgga gtacaaatcg tttctgcgtt
     1321 ttcgggtagg caaaattctt gacgatctgt gtgcgaatca gctgcaacca ttgctgttga
     1381 agaccctgct aaaccgtgct gaaggtgcgc tgttgatcaa tgcggtgggt atcgatgatg
     1441 tggcgcaggc agatgagatg gtgaagctgg caacggcggt agcgcatctg attggtcgct
     1501 caaatttcga tgcgatgagc ggtcagtatt acgcgcgttt cgtggtgaaa aatgtcgata
     1561 actcagacag ctatctgcgt cagccgcacc gcgtgatgga actgcacaac gacggcacgt
     1621 atgtcgaaga gatcaccgat tacgtgctga tgatgaaaat cgatgagcaa aacatgcagg
     1681 gtggaaattc gttgctgctg catctcgatg actgggaaca tctggaccac tatttccgcc
     1741 atccactggc gcgtcgcccg atgcgctttg ccgcgccgcc gagcaaaaac gtcagcaaag
     1801 atgttttcca tccagtgttc gacgttgatc aacagggtcg cccggtgatg cgctatatcg
     1861 accagttcgt ccagccaaaa gattttgaag aaggcgtgtg gttgagcgag ctttccgatg
     1921 ccattgaaac cagcaaaggc attctttctg tacccgttcc cgttggcaaa ttcctgttga
     1981 taaacaacct gttctggctg cacggtcgcg accgctttac tccgcacccg gatctgcgcc
     2041 gtgaactgat gcgtcagcgt ggctatttcg cttacgccac tcaccactac cagacgcatc
     2101 agtaagcgca aaggaattga gcggatgtat gattttgtga ttattggcgg cggcatcatc
     2161 ggcatgtcga ccgccatgca actgattgat gtttatccgg atgcccgcat tgcgttgctg
     2221 gaaaaagagt ccggcccggc ctgtcaccag acgggccaca acagcggcgt gatccatgcc
     2281 ggggtctatt acacgcccgg cagcctgaag gcgcagtttt gcctggcggg aaaccgcgcc
     2341 accaaagcct tttgcgatca aaacgacatt cgctatgaca actgcggcaa gatgctggtc
     2401 gccacgtccg atctcgaaat ggaacggatg cgcgcgttgt gggaacgcac ggcggcgaac
     2461 ggtatcgagc gcgagtggtt aaacgccgag gaactgcgcg agcgcgaacc gaatatcacc
     2521 gggctcggcg gcatttttgt gccgtccagc ggcattgtca gctatcgcga agtcacagcg
     2581 gcaatggcaa aaatcttcca ggccagaggc ggtgagatta tttataacgc cgaagtcagc
     2641 gccctcagtg agcataaaaa cggcgtggtc gtccgaaccc gtcagggcgg cgaatatgaa
     2701 gcatcgacgc tgattagctg ttccgggctg atggctgacc ggctggtgaa aatgctcggc
     2761 ctcgaaccgg gttttattat ctgcccgttc cgtggcgagt atttccgcct tgcgccggag
     2821 cataaccaga ttgttaacca tctgatttac cccattcccg acccggcaat gccatttttg
     2881 ggcgtgcatc tcacccgaat gatcgatggc agcgtcaccg tcgggccaaa cgcggtgctg
     2941 gctttcaaac gcgaaggcta tcgcaagcgc gacttctcat ttagcgacac gctggagatt
     3001 ttgggctcgt cggggattcg ccgggtgctg caaaaccatc tacgctcagg actgggcgag
     3061 atgaaaaact cgctgtgcaa aagcggctat ctgcgcctgg tgcaaaagta ttgtccccgg
     3121 ctttcgttaa gcgatctcca gccctggccc gccggtgtgc gggcgcaggc agtatcgccg
     3181 gacggcaagc tgattgacga ttttctgttt gtcaccaccc cacgcacgat ccacacctgc
     3241 aatgcgccct ccccggcagc gacatcagca attcctattg gtgcgcatat tgtcagcaag
     3301 gtacaaacgc tgttggcaag ccagagtaac cccggacgca cgctgcgagc ggcacgtagt
     3361 gtggatgcct tacacgccgc atttaatcaa taacctttga aaacaggacg taacaatgaa
     3421 acttaacgac agtaacttat tccgccagca ggcgttgatt aacggggaat ggctggacgc
     3481 caacaatggt gaagtcatcg acgtcaccaa tccggcgaac ggcgacaagc tgggtagcgt
     3541 gccgaaaatg ggcgctgatg aaacccgcgc cgctatcgac gccgccaacc gcgccctgcc
     3601 cgcctggcgt gcgctcaccg ccaaagaacg cgccaacatt ctgcgcaact ggttcaattt
     3661 gatgatggag catcaggacg atttagcgcg cctgatgacc ctcgaacagg gcaaaccact
     3721 ggccgaagcg aaaggtgaaa tcagctacgc cgcctctttt attgagtggt ttgccgaaga
     3781 aggcaaacgc atttatggcg acaccattcc cggtcatcag gccgataaac gcctgattgt
     3841 tatcaagcag ccgattggcg ttaccgcagc tatcacgccg tggaacttcc cggcggcgat
     3901 gattacccgt aaagccggtc cggcgctggc ggcaggctgc accatggtgc tgaagcccgc
     3961 cagtcagaca ccgttctctg cgctggcgct ggcggagctg gcgattcgcg cgggcattcc
     4021 ggctggcgtg tttaacgtgg tcaccggttc ggcaggcgcg gtcggtaatg aactgaccag
     4081 caacccgatg gtacgcaaac tgtcgtttac cggttcgacc gaaattggcc gccagttaat
     4141 ggaacagtgc gcgaaagaca tcaaaaaagt gtcgctggag ctgggcggca acgcgccgtt
     4201 tatcgtcttt gacgatgccg atctcgataa agccgtggaa ggcgcgctgg cctcgaaatt
     4261 ccgcaacgcc gggcaaacct gcgtctgcgc caaccgttta tacgtgcagg acggcgtgta
     4321 tgaccgcttt gccgaaaaat tgcagcaggc ggtgagcaaa ctgcacatcg gcgacgggct
     4381 ggataaaggc gtcaccatcg ggccgctgat cgatgaaaaa gcggtagcaa aagtggaaga
     4441 gcatattgcc gatgcgctgg agaaaggtgc gcgtgtggtt tgcggcggta aagcggatga
     4501 acgcggcggc aacttcttcc agccgaccat tctggtggac gtgccggcca acgccaaagt
     4561 gtcgaaagaa gagacgttcg gccccctcgc cccgctgttc cgctttaaag atgaagccga
     4621 tgtgattgcg caagccaatg acaccgagtt tggccttgcc gcctatttct acgcccgtga
     4681 tttaagccgc gtcttccgcg tgggcgaagc gctggagtac ggcatcgtcg gcatcaatac
     4741 cggcattatt tccaatgaag tggccccgtt cggcggcatc aaagcctcgg gtctgggtcg
     4801 tgaaggttcg aagtatggca tcgaagatta cttagaaatc aaatatatgt gcatcggtct
     4861 ttaactggag aatgcgaatg aacagcaata aagagttaat gcagcgccgc agtcaggcga
     4921 ttccccgtgg cgttgggcaa attcacccga ttttcgctga ccgcgcggaa aactgccggg
     4981 tgtgggacgt tgaaggccgt gagtatcttg atttcgcggg cgggattgcg gtgctcaata
     5041 ccgggcacct gcatccgaag gtggtggccg cggtggaagc gcagttgaaa aaactgtcgc
     5101 acacctgctt ccaggtgctg gcttacgagc cgtatctgga gctgtgcgag attatgaatc
     5161 agaaggtgcc gggcgatttc gccaagaaaa cgctgctggt tacgaccggt tccgaagcgg
     5221 tggaaaacgc ggtaaaaatc gcccgcgccg ccaccaaacg tagcggcacc atcgctttta
     5281 gcggcgcgta tcacgggcgc acgcattaca cgctggcgct gaccggcaag gtgaatccgt
     5341 actctgcggg catggggctg atgccgggtc atgtttatcg cgcgctttat ccttgcccgc
     5401 tgcacggcat aagcgaggat gacgctatcg ccagcatcca ccggatcttc aaaaatgatg
     5461 ccgcgccgga agatatcgcc gccatcgtga ttgagccggt tcagggcgaa ggcggtttct
     5521 acgccgcgtc gccagccttt atgcagcgtt tacgcgcgct gtgtgacgag cacgggatca
     5581 tgctgattgc cgatgaagtg cagagcggcg cggggcgtac cggcacgctg tttgcgatgg
     5641 agcagatggg cgttgcgccg gatctcacta cctttgcgaa atcgatcgcg ggcggcttcc
     5701 cgctggcggg cgtcaccggg cgcgcggaag tgatggatgc cgtcgctcca ggcggtctgg
     5761 gcggcaccta tgccggtaac ccgattgcct gcgtggcggc gctggaagtg ttgaaggtgt
     5821 ttgagcagga aaatctgctg caaaaagcca acgatctggg gcagaagcta aaagacggat
     5881 tgttggcgat agccgaaaaa cacccggaga tcggcgacgt acgcgggctg ggagcgatga
     5941 ttgccatcga gctgtttgaa gatggcgatc acagcaaacc ggacgcaaaa ctcaccgccg
     6001 agatcgtggc acgcgcccgc gataagggcc tgattcttct ctcctgcggc ccgtattaca
     6061 acgtgctgcg catccttgta ccgctcacca ttgaagacgc tcagatccgt cagggtctgg
     6121 agatcatcag ccagtgtttt gctgaggcga agcagtagct atgtccgttt gtaagagtta
     6181 aacgttgcta agggaatgat aacgatgcag taaaaaataa taacgatgag gcgatcgaca
     6241 aagttaaaaa cgactgcgga ctgtcccctc gctcctctgg ggagagggtt agggtgaggg
     6301 gtaatcacgc ggctacaccc tcaccccggc cctctcccta agggagaggg agaaaactct
     6361 cccgctttcg tttttaagat tttgttgacc gtctcaatta aacgcgtgac ccgacgcggg
     6421 aaatgtcggg gcgctctccc aagtgacaca ctttcgagag gattcaggat ggggcaatca
     6481 tcgcaaccac atgagttagg cggcgggctg aagtcacgcc acgtcaccat gttgtctatt
     6541 gccggtgtta tcggcgcaag tctgtttgtc ggttccagcg tcgccatcgc cgaagcgggc
     6601 ccggcggtat tactggccta tctgttcgcc ggattactgg tggttatgat tatgcggatg
     6661 ttggcggaaa tggcagttgc cacgcccgat accggttcgt tttccaccta tgccgataaa
     6721 gccattggcc gctgggcggg ttataccatc ggctggctgt actggtggtt ttgggtactg
     6781 gttatcccgc tggaagccaa catcgccgct atgatcctgc actcgtgggt tccaggcatt
     6841 cccatctggt tattttccct cgtcattacc ctcgccttaa ctggcagtaa tttattaagc
     6901 gttaaaaact acggcgaatt tgagttctgg ctggcgctgt gcaaagtcat cgctatcctg
     6961 gcctttattt tccttggtgc agtcgcaatt agcggttttt acccttatgc cgaagtgagc
     7021 gggatctcaa gattgtggga tagcggcggc tttatgccca acggcttcgg tgcggtatta
     7081 agcgcgatgt tgatcaccat gttctcgttt atgggcgcag aaattgtcac cattgccgcc
     7141 gcggaatccg acacgccgga aaaacatatt gtccgcgcta ccaactcggt tatctggcgt
     7201 atttctatct tctatttgtg ctctattttt gtcgtagtgg cgttaatacc gtggaatatg
     7261 ccggggctga aagccgttgg ttcttatcgc tcggttctgg aattgctcaa tattccccat
     7321 gcgaaattaa tcatggactg cgtgatatta ctttccgtaa ccagctgtct gaactcggcg
     7381 ctgtataccg cgtcaaggat gctctactcc ttaagccgtc gcggtgatgc gcccgcgcta
     7441 atgggcaaaa tcaaccgcag taaaaccccg tatgtggcgg tgttactctc caccggagcg
     7501 gcatttttaa cggtggtggt gaactattac gcacctgcga aagtgtttaa attcctgata
     7561 gacagctccg gcgctatcgc cctgctggtt tatttagtca tcgccgtttc acagttgcgg
     7621 atgcgcaaaa ttctgcgagc agaaggaagc gaaattcgct tgcgcatgtg gctttatccc
     7681 tggctcacct ggctggtcat cggctttatt acctttgtgt tggtagtgat gctattccgt
     7741 ccggcgcaac agttagaagt gatctccacc ggcttattag cgatagggat tatctgtacc
     7801 gtgccaatta tggctcgctg gaaaaagctg gtattgtggc aaaaaacacc cgttcataat
     7861 acgcgctgat aatgatcagg agtcacacca tgaccattac gtctctggat ggctatcgct
     7921 ggctgaagaa cgatattatt cgcggtaatt ttcaaccgga tgaaaaatta cggatgagtt
     7981 tgctgacatc gcgttatgca cttggcgttg ggccgttgcg ggaagcactt tctcaactgg
     8041 tggcggaacg gctggtcacg gtggtgaatc aaaaagggta tcgggtggcg tctatgtcag
     8101 agcaggagct gctcgatatt ttcgacgccc gcgccaatat ggaagcgatg ctggtcagtc
     8161 tggcgattgc ccgcggtggc gatgaatggg aggcagacgt tctcgcaaaa gcgcatctgc
     8221 tcagtaagct ggaagcctgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc
     8281 atcaggcgtt tcatacagca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc
     8341 gtgaacggtt gtttgatctg gcggcgcgct atcgatttat ctggctgcgg cgaacggtgc
     8401 tttcggtaga aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac
     8461 tggcgcgaga taccgcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc
     8521 ccattatcca gcaggcgatg gctggcaatt aactactctt ccggaatacg caacacttgc
     8581 cccggataaa ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg
     8641 tacagattag cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta
     8701 acggtataaa actggctggc agtggctggt gtcgccgttt tcacctgatc atcgacactg
     8761 gcaataccgg aaatattccc aaccgcaaca aggattttct ccttcgcctc ctgactcagg
     8821 ccgtcaccag tgaccgtcgc tttgccgtcg gcaatttgaa tgttcacttt atcggcatca
     8881 ggtataccgg ttttgctcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc
     8941 tgacctgtaa ccgcgtccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc
     9001 atagctacct cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt
     9061 taacgccgcg ggggtgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca
     9121 aaccggggat atacccaagc agtgtcagca gaatattaat aatgaacgcc caaccgaacc
     9181 ctttaccgag cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac
     9241 ccatatgtac tccctataag aaaattactc attgtttaaa aagagatttt atctcttaag
     9301 cgtagtaatt ttagcggagc ctgccagttt cagcttacgc ttttactctc at
//
LOCUS       DAZGOB010000163         9117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGOB010000163 DAZGOB010000000
VERSION     DAZGOB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.163"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            285..1343
                     /gene="citC"
                     /locus_tag="ACHLIF_004448"
     CDS             285..1343
                     /gene="citC"
                     /locus_tag="ACHLIF_004448"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO9608331.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1358..1654
                     /gene="citD"
                     /locus_tag="ACHLIF_004449"
     CDS             1358..1654
                     /gene="citD"
                     /locus_tag="ACHLIF_004449"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO9608332.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1651..2559
                     /gene="citE"
                     /locus_tag="ACHLIF_004450"
     CDS             1651..2559
                     /gene="citE"
                     /locus_tag="ACHLIF_004450"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO9608333.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2570..4102
                     /gene="citF"
                     /locus_tag="ACHLIF_004451"
     CDS             2570..4102
                     /gene="citF"
                     /locus_tag="ACHLIF_004451"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO9608334.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLATFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4106..4657
                     /gene="citX"
                     /locus_tag="ACHLIF_004452"
     CDS             4106..4657
                     /gene="citX"
                     /locus_tag="ACHLIF_004452"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO9608335.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4632..5510
                     /gene="citG"
                     /locus_tag="ACHLIF_004453"
     CDS             4632..5510
                     /gene="citG"
                     /locus_tag="ACHLIF_004453"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO9608336.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5561..7024
                     /gene="citT"
                     /locus_tag="ACHLIF_004454"
     CDS             5561..7024
                     /gene="citT"
                     /locus_tag="ACHLIF_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO9608337.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7138..7944
                     /gene="rna"
                     /locus_tag="ACHLIF_004455"
     CDS             7138..7944
                     /gene="rna"
                     /locus_tag="ACHLIF_004455"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO9608338.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8174..8584
                     /gene="rnk"
                     /locus_tag="ACHLIF_004456"
     CDS             8174..8584
                     /gene="rnk"
                     /locus_tag="ACHLIF_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO9608339.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8722..8811
                     /gene="yldA"
                     /locus_tag="ACHLIF_004457"
     CDS             8722..8811
                     /gene="yldA"
                     /locus_tag="ACHLIF_004457"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO9608340.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8815..>9117)
                     /locus_tag="ACHLIF_004458"
     CDS             complement(8815..>9117)
                     /locus_tag="ACHLIF_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608341.1"
                     /translation="FIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEE
                     IVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2127 a         2458 c         2321 g         2211 t
ORIGIN      
        1 tattcattaa atcaattaaa tcacttaaac ctgctggtca tttcgtaatt acattaatta
       61 aaaaacttaa aaccattaaa taaataaaaa accactaact ctatgtgaaa taaatcaaaa
      121 tttcacgccg aaatactcct taggatgtat agcgaaagga gaaaaagata tacctcgatc
      181 accccccttt ctcccaagtg aaaataaaag gttatcagtt tgcaacattg aacaacattc
      241 gttgcaaatc gataacaaca tgcaccttca ggatactatt tattatgttc ggcaatgata
      301 ttttcacccg cgtaaaacgt tcagaaaata aaaaaatggc ggaaatcgcc caattcctgc
      361 atgaaaatga tttgagcgtt gacaccacag tcgaagtatt tattaccgta acccgcgatg
      421 aaaagcttat cgcgtgcggt ggaattgccg gaaatattat taaatgcgtt gctatcagtg
      481 aatccgttcg cggtgaagga ctggcgctga cattagccac tgaactgata aacctcgcct
      541 atgagcggca cagcacgcat ctgtttattt ataccaaaac cgaatacgag gcgctgttcc
      601 gccagtgcgg tttttccacg ctgaccagcg tacccggcgt gatggtgctg atggaaaaca
      661 gcgccacgcg gctgaaacgc tatgccgaat cgctgaaaaa atttcgtcat ccagggaaca
      721 agattggctg cattgtgatg aacgccaatc ccttcacgaa tggtcaccgt tatctgattc
      781 aacaagctgc ggcacagtgc gactggttgc atctgttttt agtcaaagaa gattcttcac
      841 gcttccccta tgaagaccgg ttggatctgg tgttaaaagg caccgccgat attccacgcc
      901 tgactgtgca tcgcggctcc gaatacatca tctcccgcgc tacgttccct tgctacttca
      961 ttaaagaaca gagcgtcatt aaccattgtt acaccgaaat tgatctgaag attttccgtc
     1021 agtacctcgc tcccgcgctg ggtgtaactc accgctttgt cggtactgaa cccttttgtc
     1081 gcgttaccgc ccagtacaac caggatatgc gctactggct ggaaacgccg actatctccg
     1141 caccgcccat cgaactggtt gaaattgagc ggctgcgtta ccaggagatg ccgatatccg
     1201 cttcccgggt acgtcaactg ctggcgaaaa acgatctcac ggctatcgcg ccgctggtcc
     1261 ctgcagtcac gctgcattat ttgcagaacc tgcttgagca ctcccgccag gacgcggcag
     1321 ctcgtcaaaa gacccccgca tgagaaacag gtgaaaaatg aaaataaacc agcccgccgt
     1381 tgcaggcacc cttgagtctg gggatgtgat gatacgcatc gccccactcg atacgcagga
     1441 tatcgacctg caaatcaata gcagcgttga gaaacagttt ggcgatgcaa ttcgcaccac
     1501 cattctggac gttctcgccc gctacaacgt gcgcggcgta cagctgaatg tcgatgacaa
     1561 aggcgcactg gactgcattt tacgtgcacg actggaagcc ctgctggcac gcgccagcgg
     1621 tatcccggct ctgccatggg aggattgcca atgatttccg cttcgctgca acaacgtaaa
     1681 actcgcaccc gccgcagcat gttgtttgtg cctggtgcca atgccgcgat ggtcagcaac
     1741 tccttcatct acccggctga tgccctgatg tttgacctcg aagactccgt ggcattacgt
     1801 gaaaaagaca ccgcccgccg catggtttat cacgcgctgc aacatccgct gtatcgcgat
     1861 attgaaacca ttgtgcgtgt caacgcgctg gattccgaat ggggtgttaa cgacctggaa
     1921 gccgtcgttc gcggtggtgc ggacgttgtg cgtctgccga aaaccgatac cgctcaggat
     1981 gttctggata ttgaaaaaga gatcctgcgt atcgaaaaag cctgtggtcg tgaacccggc
     2041 agcaccggcc tgctggcggc gattgaatct ccgctgggca ttacccgcgc agtggaaatc
     2101 gctcacgctt ccgagcgttt gatcggtatc gccctcggtg cagaagacta tgtgcgcaac
     2161 ctgcgtacag aacgctcccc ggaaggaact gaactgctgt tcgcacgctg ttccattttg
     2221 caggccgcgc gctctgcggg tattcaggcg ttcgataccg tctattccga cgctaacaac
     2281 gaagccggat tcctgcaaga agccgcccac atcaaacagc tgggctttga cggcaaatcg
     2341 ctgatcaacc cgcgtcagat tgatctgttg cacaacctct acgcaccaac ccagaaagaa
     2401 gtggatcacg cccgccgcgt cgtagaagcc gctgaagccg ccgctcgcga aggcctcggc
     2461 gtggtttccc tgaacggcaa gatggtggac ggtccggtta tcgatcgcgc ccgtctggtg
     2521 ctctctcgtg cagaactttc cggcatccgc gaagaataag gcaatcaaaa tgacgcagaa
     2581 aattgaacaa tctcaacgac aagaacgggt agcggcctgg aatcgtcgcg ctgaatgcga
     2641 tcttgccact ttccagaact cgccaaagca aacctaccag gctgaaaaag cgcgcgatcg
     2701 caaactgtgc gccaacctgg aagaagcgat tcgtcgctct ggtttacagg acggcatgac
     2761 ggtttccttc catcacgctt tccgtggcgg tgacctgacc gtcaatatgg tgatggacgt
     2821 catcgcgaag atgggcttta aaaacctgac cctggcgtcc agctccctga gtgattgcca
     2881 tgcgccgttg gtagaacaca ttcgccaggg cgtggttacc cgcatttata cctccggcct
     2941 gcgcggtcca ctggcggaag agatctcccg tggtctgctg gcagaaccgg tgcaaatcca
     3001 ctctcacggc ggtcgcgtgc atctggtaca gagcggcgaa ctgaatatcg acgtggcttt
     3061 cctcggcgtc ccgtcctgcg atgaattcgg taatgccaac ggctacaccg gtaaagcctg
     3121 ctgcggctcc ctcggctatg caatggttga tgccgacaac gcaaagcagg tcgtgatgct
     3181 taccgaagaa ctgctgcctt atccgcataa tccggcaagc attgagcaag atcaggttga
     3241 tttgatcgtc aaagttgacc gcgttggcga tgctgcaaaa atcggcgctg gcgcgacccg
     3301 tatgaccact aacccgcgcg aactgcttat tgcccgtagc gctgcggatg tgattgtcaa
     3361 ctcaggctac ttcaaagaag gtttctccat gcaaaccggc accggcggcg catcgctggc
     3421 ggtaacccgt ttcctggaag acaaaatgcg tagccgcgat attcgcgccg acttcgccct
     3481 tggcggtatt accgcgacga tggttgacct gcacgaaaaa ggtctgatcc gcaaactgct
     3541 ggatgtgcag agctttgaca gccatgctgc gcaatcgctg gcccgtaacc ccaatcacat
     3601 cgaaatcagc gccaaccagt acgctaactg gggttcgaaa ggcgcatcgg ttgatcgtct
     3661 cgacgtggtg gtactgagcg cgctggaaat tgacacccag ttcaacgtta acgtgctgac
     3721 cggctctgac ggcgtactgc gtggtgcttc cggtggtcac tgcgataccg cgattgcctc
     3781 tgcgctttcc atcatcgtcg cgccgctggt acgcggtcgt attccgactc tggtggataa
     3841 cgtgctgacc tgcatcaccc cgggctccag tgtcgatatt ctggtcacag accacggtat
     3901 cgcagttaac ccggcacgtc cggaactggc agaacgtctg caggaagcgg gcattaaagt
     3961 ggtttccatt gagtggctgc gcgaacgtgc gcgtctgctg accggtgaac cacagccgat
     4021 tgaattcacc gaccgcgtcg ttgccgttgt gcgttaccgc gatggctcgg tgatcgatgt
     4081 tgtgcatcag gtgaaggaat aagccatgca cctgcttcct gaactcgcca gccaccatgc
     4141 ggtgtcaatt cccgagctgc tcgtcagccg ggatgaaagg caagcacggc aacacgtctg
     4201 gctcaagcgc catcctgttc cactggtctc ctttaccgtg gttgcgcctg ggccgattaa
     4261 agacagcgag gtcacacgcc gaatttttaa tcatggcgtg acagccttgc gtgccttagc
     4321 cgcaaaacag ggctggcaaa ttcaggagca ggctgcactg gtttccgcca gcgggccgga
     4381 gggcatgttg agcattgccg ccccggctcg cgacctcaag ctcgccacca ttgagcttga
     4441 acatagtcat cctctcgggc ggttatggga tatcgatgtc ctgacgcccg aaggcgaaat
     4501 tctctcccgc cgcgactatt cactgccgcc tcgccgctgc ctgttgtgcg agcaaagcgc
     4561 agccgtctgc gcgcgtggaa aaacccatca actgaccgat ttactcaacc gcatggaggc
     4621 actgctgaac gatgtcgatg cctgcaacgt caactaaaac cacaaagctt gcgacgtcat
     4681 taatcgatga gtacgccctg ctgggctggc gcgccatgct gactgaagtc aatctgtcac
     4741 cgaaaccagg cctcgtggat cgcattaact gcggtgcgca caaagatatg gcgctggaag
     4801 atttccaccg cagcgcgctg gcgattcagg gctggttacc ccgtttcatt gaatttggtg
     4861 cctgtagtgc agaaatggca ccggaagcgg tactccacgg attacgccca attggtatgg
     4921 cttgcgaagg tgatatgttc cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt
     4981 tttctttagg gctgctatgt gcggcaattg gccgtttgct tcagctcaac cagccggtaa
     5041 cgccaacaac cgtttgttct acggcggcaa gtttctgccg tggtctgacc gatcgcgaac
     5101 tgcgtaccaa taattcacaa ctgacggcag gtcaacggtt gtaccagcag cttggcctta
     5161 ccggcgcacg cggtgaagcc gaagcgggtt atccactggt gatcaaccac gccttgccgc
     5221 attacctcac tctgctggat caggggttag atcctgaact ggcattgctc gataccttgc
     5281 tcctactgat ggcgatcaac ggcgatacca acgttgcttc gcgcggtggc gaggggggcc
     5341 tgcgctggct acagcgcgag gcgcaaacat tattgcaaaa agggggcatt cgaacccccg
     5401 ccgatctcga ttatctccgg cagttcgaca gggagtgtat cgaacgaaat ctcagtccag
     5461 gcggcagtgc cgacctgctg atccttacct ggtttttagc acagatttaa ttatttaagc
     5521 acttgataaa tttgaaaata ttaattttcg gagaacccgt atgtctttag caaaagataa
     5581 tatatggaaa ctattggccc cactggtggt gatgggtgtc atgtttctta tccctgtccc
     5641 cgacggtatg ccaccacagg catggcatta cttcgctgtg tttgtggcaa tgattgtcgg
     5701 catgatcctc gagccaattc cggcaacagc gatcagtttt attgcggtta ctatttgcgt
     5761 tattggcagt aattacctgc tctttgatgc caaagaatta gctgacccag cgtttaatgc
     5821 gcaaaaacag gcgctgaaat ggggtctggc tggtttttcc agcaccacgg tatggctggt
     5881 atttggcgca tttatttttg cattagggta tgaagtttcc gggttaggtc gtcgcattgc
     5941 ccttttcctg gtgaaattca tgggcaaacg cacgctgacg ttgggttatg cgattgtcat
     6001 tatcgacatt ctgctggcac cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt
     6061 ttttccggtc attaaaaacc tgccgccgtt gtttaaatca ttccccaacg atccgtccgc
     6121 gcgtcgtatt ggcggctatt tgatgtggat gatggtcatt agtaccagtc tgagttcgtc
     6181 catgtttgtc accggtgcgg caccaaacgt gctgggtctg gagttcgtca gcaaaattgc
     6241 cggtatccag attagctggt tgcagtggtt cctctgcttc ctgccggttg gggttatctt
     6301 gcttatcatt gcgccgtggc tttcctacgt gctgtacaaa ccggaaatca cacacagtga
     6361 agaagtggca acctgggcgg gtgatgaact gaaaaccatg ggtgcgctga cacgcagaga
     6421 gtggacactg attggccttg tattgctcag cttaggtttg tgggtttttg gcagcgaagt
     6481 cattaatgct actgcagttg gtctgctggc agtttcgcta atgctggccc tgcacgttgt
     6541 gccttggaaa gatattaccc gctataacag cgcatggaac acgctggtca acctagcaac
     6601 tctggttgtg atggctaacg gcctgactcg ttctggcttt attgactggt tcgccggtac
     6661 catgagtacg cacctggaag gattctcacc aaacgcaacg gtgattgtac tggttctggt
     6721 gttctacttt gcacactacc tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc
     6781 ggttattctg gccgtcggta aaggtattcc gggcgtacca atggaacaac tgtgtatcct
     6841 gctggtgctg tctatcggta tcatgggctg tctgacgccg tatgcaaccg gtcctggggt
     6901 gattatttac ggctgtggct atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt
     6961 cggggtgatt tacatctcta tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa
     7021 ctaatacgtt cgccaaacgc tgaataaatc atcacgcccg ccaggtaagc ccacctggcg
     7081 ggctttttta tgatttaata gatagtattc agtgtatgta tgagttccac acccattatg
     7141 aaagcattct ggcgtaacgc cgcgttgctc gcggtttctc tgcttccctt ctcttctgcc
     7201 aacgccttag cgttgcaggc aaaacagtat ggcgattttg atcgctatgt cctggccctc
     7261 tcctggcaaa ccggattttg ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa
     7321 tgtcgcctgc aaaccgaaac gaccaacaaa gctgattttc tgaccgtaca tggtctgtgg
     7381 ccaggattgc ctaaatcggt tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc
     7441 ggttgcgcta ctcgcccaat cccgaatcta ccagaagcgc acgccagccg gatgtgttca
     7501 tcgccggaaa ccggattatc actggaaacg gccgctaaac taagtgaagt catgccagga
     7561 gctggcggac gttcctgcct ggaacgctac gaatatgcca aacacggtgc ctgctttggt
     7621 tttgatccgg acgcatactt cggtacgatg gtacgcctga atcaagaaat taaagagagc
     7681 gaagccggaa aattccttgc ggataattac ggtaaaacag tgagccgccg tgactttgac
     7741 gccgcctttg ccaaaagctg gggaaaagag aacgtgaaag cagttaagct aacgtgccag
     7801 ggtaaccctg cgtatttgac tgaaattcag atctcgatca aagctgacgc aatcaacgct
     7861 ccgctttctg caaactcatt cttgccacaa ccacacccag gcaactgtgg caaaaccttt
     7921 gtgattgata aagtgggtta ttaatcatct gatttacaaa acacgctaat tcgtcttcca
     7981 taaaccgtga cgattaagaa ttatacataa tgcgctgcat tgaggaattc cctgaatgtg
     8041 acgcaaatca cttcaaagag tgagcaacag tcagtatcat catgtgagtt aaaccctcgc
     8101 cgcctgacgg tgagggtttt cttttgggta tgtttatctg cgacactcgc agtaccgacg
     8161 acatggagta aaaatgtcca gaccaactat catcattaac gacctggatg ccgaacgcat
     8221 cgatattctg ctggagcaac ccgcctatgc tggtttgcca atcgccgacg cgttaaacgc
     8281 agagttggat cgcgcccaaa tgtgttcgcc agaagagatg ccacacgacg tggtgacaat
     8341 gaacagccgg gttaaattcc gcaatcttag cgatggcgaa gtgcgtgtgc gcacgctggt
     8401 gtatccggca aaaatgaccg atagcaatac tcagctttcc gttatggctc cggtaggtgc
     8461 cgcactgctg gggctgcgcg ttggcgattc cattcactgg gaacttccgg gcggcgttgc
     8521 aacccacctt gaagtgctgg aactcgaata ccagccagaa gctgctggcg actacctgct
     8581 ttaatcccat ctgcacaagc gataactcgc tttgcccaga ggatgcactc gcatcctctt
     8641 tacgcaaaat cagcaatttt tcctggttct cactgttcac ttttgctgca aaattattcc
     8701 gtttcgtttg tgggaataac catggcagaa gcattttaca tcctgatagg atttctgatt
     8761 atggcggcga ttatcgtcat ggctgtcctt tacctcgaaa accactcctg acgatcatat
     8821 tgttcccccc ggcatcgcat tcaccagacc tgaaaccgcc ttctgcgccg cctctgcgct
     8881 ttgtgcaccg ggtaccagaa tcaccttacg gcactcctct tcacgttttt cgaaaatctc
     8941 atatccccgg gcggcctctt caaacggcat atagtgggta acaatttctt ctggtttcag
     9001 taatcctttc tcaattaacg gtaataattc tcccagccat gcgtgaacgt gggtctgtcc
     9061 cattttaaac gtcaaccctt tatcaaaggc gtcgccaaac aggaaaccgt gaataaa
//
LOCUS       DAZGOB010000164         9105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGOB010000164 DAZGOB010000000
VERSION     DAZGOB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.164"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1371)
                     /locus_tag="ACHLIF_004459"
     CDS             complement(<1..1371)
                     /locus_tag="ACHLIF_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO9608342.1"
                     /translation="MIHPGSFLDKTINNTRGKNVNANIKHSQIQEIKRNFTYKKNDDI
                     SSRFKFYSSLVKQKNSTEDVVLIGKMISDEVRNYSTIHNDRNIIINSGNWKTSFLCNL
                     ARLLYSMFNGSVYFPSRDDENSSSPSPTLPTIHQSERKELSQQKSINHLKKSDTIDEY
                     IKIRKTRGIEDQITTTSQSLIINELLNGVNRNTIPFQKISELNDIIHSYENMQTKNSR
                     KGIEILVKQGELLSSLINYDKENKQLSDNISKIINLLGIEYQSHKVDIEPFIHAVWVA
                     GAPPENTSSYITAFLNTYKNYTYLLWIDPNAFGAAKFSGILKNIAMNYAIMRLRRTNP
                     HLAEKMNDVILKIQHIQNETVDFNETRERVKELENKYKSLTRETKEKFNVFFLESMIG
                     MQDNYFTYCISNGISNTDDISRLNFLTTVLKLSPDILDDFKSTVEKNKKDIDLLKDII
                     SQKFGDR"
     gene            complement(1486..1716)
                     /locus_tag="ACHLIF_004460"
     CDS             complement(1486..1716)
                     /locus_tag="ACHLIF_004460"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9608343.1"
                     /translation="MNCWRCFPLFFIGILTTDEWGSHIRYVIEGDSSIFIQHIECMRI
                     LIKHLTRRAICFPHSVNFIKKHRYFIEKYMFY"
     gene            1639..2131
                     /locus_tag="ACHLIF_004461"
                     /pseudo
     CDS             1639..2131
                     /locus_tag="ACHLIF_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558155.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2198..2326)
                     /locus_tag="ACHLIF_004462"
     CDS             complement(2198..2326)
                     /locus_tag="ACHLIF_004462"
                     /inference="COORDINATES: protein motif:HMM:NF025189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO9608344.1"
                     /translation="MFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPE"
     gene            complement(2357..3123)
                     /locus_tag="ACHLIF_004463"
                     /pseudo
     CDS             complement(2357..3123)
                     /locus_tag="ACHLIF_004463"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(3159..3698)
                     /locus_tag="ACHLIF_004464"
     CDS             complement(3159..3698)
                     /locus_tag="ACHLIF_004464"
                     /inference="COORDINATES: protein motif:HMM:NF024408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO9608345.1"
                     /translation="MLTTSESQFRQKDQQLSLVEETEAFLCSALARAEEKIEEDEREI
                     EHLQTQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDAPQVPRQLRQ
                     PRHRRPLPAHLPHAIHRLESEESCCPECGGELDYLEEVSAEQLELVSSALKVIRTVRV
                     KKPVQNVTASLKHRRRPAR"
     gene            complement(3710..4862)
                     /locus_tag="ACHLIF_004465"
                     /pseudo
     CDS             complement(3710..4862)
                     /locus_tag="ACHLIF_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
     gene            complement(4843..5319)
                     /locus_tag="ACHLIF_004466"
     CDS             complement(4843..5319)
                     /locus_tag="ACHLIF_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9608346.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACMAQIARENGVND
                     DVIFKWLRLWQNEGRISRRLPVTTTSDAGVELLPVEITPDEPKEPMAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVKWHTEFGHLNRGDMLTSEQHRCSNEKKKF"
     gene            5406..5520
                     /locus_tag="ACHLIF_004467"
                     /pseudo
     CDS             5406..5520
                     /locus_tag="ACHLIF_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5571..7080)
                     /locus_tag="ACHLIF_004468"
                     /pseudo
     CDS             complement(5571..7080)
                     /locus_tag="ACHLIF_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7700..8707)
                     /locus_tag="ACHLIF_004469"
                     /pseudo
     CDS             complement(7700..8707)
                     /locus_tag="ACHLIF_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         2250 c         2040 g         2641 t
ORIGIN      
        1 tctatccccg aatttttgag atattatgtc ttttaataaa tctatatctt ttttgttttt
       61 ctctactgta gatttaaagt catccagaat gtctggtgaa agttttaaaa cagtagttaa
      121 aaagttaagt ctggatatat catcagtatt tgatattccg tttgatatac aatatgtgaa
      181 atagttgtct tgcataccta tcattgactc aagaaaaaaa acattgaatt tttctttggt
      241 ttctctcgtt aatgatttgt atttattttc aagttccttt accctctctc ttgtttcatt
      301 aaaatctact gtttcatttt gtatgtgttg tatttttaat atcacatcat tcattttttc
      361 cgctaaatga ggattggttc ttctcaaacg cataattgca taattcatgg ctatattttt
      421 taaaattcca ctaaacttgg ctgcaccaaa agcattggga tcaatccaga gcaagtaagt
      481 ataattttta tatgtattta aaaaagctgt tatatatgac gatgtatttt caggaggtgc
      541 acctgcaacc catactgcat gtataaatgg ctctatgtct actttatgtg actgatactc
      601 tatccctaat aaatttatta ttttagatat attgtctgat aattgtttgt tttctttgtc
      661 ataatttatt aatgatgata acagttctcc ctgcttaact aatatttcta taccttttcg
      721 gctattttta gtttgcatat tttcatatga atgtatgatg tcattgagct cacttatttt
      781 ctgaaatgga atggtatttc tattaactcc atttaacagc tcattaatta tcaaactttg
      841 agaggtagtt gttatttgat cttcaatgcc tcttgttttt cttattttaa tgtattcgtc
      901 gatggtatca ctttttttta aatgattgat gcttttttgt tgtgataact ccttcctttc
      961 agactgatgt atagtaggta gagttgggct gggggatgag ctattttcat catcacggga
     1021 aggaaaatag acgctaccat taaacatgct atatagtagt ctggcaagat tacataaaaa
     1081 agatgttttc caatttccag agttaattat gatatttcta tcattatgta tggtgctgta
     1141 atttctaact tcatctgaaa tcatttttcc tatcaaaaca acatcttctg ttgagttttt
     1201 ctgctttact agcgagctgt aaaatttaaa tctggatgag atgtcatcat tttttttata
     1261 ggtgaaattc ctttttatct cttgaatttg actatgtttg atattagcat taacattttt
     1321 acctcttgta ttgttaattg tcttatctaa aaaagaacct ggatgaatca tatgtatact
     1381 ctctagttac gcaacaatta atgaattttg attggtgaca acctcaaatg tattttgagg
     1441 ttgttatata aaacacatta ttttatatga tttggtgata tccaattagt agaacatgta
     1501 tttttcgata aagtaccggt gtttttttat gaaatttaca gagtgcggga aacagatagc
     1561 cctacgggtc agatgtttga ttaatattct catgcactca atatgctgaa taaaaataga
     1621 tgagtctcct tctataacat atctgatgtg actaccccat tcgtcagtgg tcaatatacc
     1681 aatgaaaaaa agagggaagc agcgccagca attcacaaca gtataggtat tgctgctgca
     1741 gcccgtgagc tcagcctgta tgaatcacag tctacaactg ggtagtcaac aacaaaatca
     1801 gtagacgttc ttctgaacgt gagctggaga tgtctaccga tgttgcacgt ctcaaacgcc
     1861 agctggcgga acgggatgaa gagctgacca tcctccaaaa ggccgcgaca tacttcgcga
     1921 agcgcctgaa atgaagtatg tctttattga aaaacatcag gctgtgttca acatcaaggc
     1981 aatgtgccat gtgctccagg aaggccgcag tgactggtat atgtggtgtc agcggcggac
     2041 aaggataagt acatgtcagc agttccgtca gcactgcgac agcgtggtac tcgcggcttt
     2101 tatccggtca aaacagcgct acggagcacc gtattgacgc ttatttattg gtgagtacta
     2161 cgttccatgg caggagttcg tcaacacggt tggagggcta ttccggtagt acgctcagaa
     2221 tatggcgcag atacgcttcc ggatcgatac cgttcagacg gcaggtgccg atcagcccgt
     2281 acagcagtgc tccacgctcg ccgccgtgat cgctaccgaa gaacatgtaa ttctttttcc
     2341 cgagacagac ggcacgaagc gctctttccg ctgtgttatt gtccgcctcc gccagaccgt
     2401 catcactgta ataacagagg gcgtcccact gattcagtac atagctgaac gcttcgcctg
     2461 gtctggattt tttcgacagc gtgccattct tctccaccat ccattcatgc agcgacgtca
     2521 gtaacgcttt gcttcgctgc tgcctgactt caagacgctc tgactctggt aatccccgta
     2581 tttcatcctc gatggcgtac agttcactga tgcgcttcag ggcttcttct gccgtcgcac
     2641 ttttgctgct gatgtataca tcgtggattt ttctccggac atgggctcag cacgcaactt
     2701 ctgtcagcgc accatcttca cgttctgcac tgaacagccg atcgtaaccg ttgaacgcat
     2761 ccgcctgcag ggggcggagg tgttgctccg ggtgtttccc ctgccggttc ggtgagtacg
     2821 cgaaccagac cgccggagga gatgacgaac ccgcattgcg atcattccgg acatacgtcc
     2881 agatgcgccc tgttttcgtc ttttcaggcc cagtgccagt acctttaccg gtgtgtcgtc
     2941 agtgtgaagc ttgcgggtat tcatcacata acggtacagg gcatcattca gcggagtcat
     3001 taactggcag cacgcgtcaa cccagttgga gagtaatgca cggctcagtt cgacaccctg
     3061 tcgggcaaag atttcactct gacgatacag tggcagctgt tcgcagtatt ttcccgttaa
     3121 cacgcgggca agtaatccgg ggcccgcgat accacgctct atcgggcggg acggcgccgg
     3181 tgcttcaacg atgcagtcac attttgtaca ggctttttta cccgaacagt gcggatcact
     3241 ttcagggcgc tgctcaccag ttccagttgt tctgcactga cttcctccag ataatccagc
     3301 tcaccgccac actccgggca acagctttct tcagactcca gacggtgtat tgcatgggga
     3361 aggtgtgccg gtaacggacg acgatggcgc ggctgtctca actggcgggg aacctgtgga
     3421 gcgtcttccc gcccactgta acgatcgctg tcctgttcgc gttgtttcag cagggcctca
     3481 gcctgttcaa cttcacgacg cagtttttca gaacgggtac cgaacagcat ccggcgcagt
     3541 ttttctatct gagtctgcag atgttctatt tcccgttcat cttcttcgat cttttcttcg
     3601 gcacgtgcca gtgcagagca caggaaggcc tccgtctctt caaccagact tagttgctgg
     3661 tctttctgac ggaactggct ttctgaagtg gtcaacaaaa actggccacc tagttagagt
     3721 ttttccagta tcgattttcc gattcgtttg ggggtaaccc accgttatat tcgtgcggtc
     3781 ttagtgcgct gtaatatcca acgatatagt ccgttatggc gtgagctgcc tcgctgaagc
     3841 ttacgtaacc caccaccggc atccattcgt tctcttcaga ctcctgaaga agcgttccat
     3901 tgggctgtta tcccagcagt ttccgcgccg gctcatactc tgtctgatct ggtatcgcca
     3961 caataactgc cggaactgcc tgctcgtata atgactgccc tgatcgctgt ggaacatcac
     4021 cccgccgggc ttaccacggg tttcccatgc catttcccgc gctttcatgg tgagcctgct
     4081 gtccggcgag aacgacatgg cccagcccac tggttttctt gcgaacaggt cgagaacaac
     4141 ggcgaggtac gcccagcgct tacccgtcca gatataggtc acatcaccgc accacacctg
     4201 atttggctcg gtcacggcga actgcctttc aaggtagtta gggatagcaa catgttcatg
     4261 accaccacgt ttataccggt gagtcggctg ctgacagctg accagcccca gctctttcat
     4321 gagcctgcca gcaagccagc gtcccatctg gtagcctctc cgggttgcca ttgtggcgat
     4381 gcttcttgct ccggccgaac cgtggctgat gccatgtagc tcaagtacct gactgcgtaa
     4441 tacagcccgt ctgccgtctg gtttttcagg acggtttttc cagtatctgt agctgctgcg
     4501 atgaaccccg aacacatggc agagtgtgac cacaggataa tgcgctctga gtttcccgat
     4561 tatcgagaac tgttcaggga gtctgacatc aagagcgcgg tagccttttt taatatttca
     4621 ttctccattt caatgcgttg tagctttttc ctcagcttac gtatttcgat ttgttctggt
     4681 gttatcggag aggcttttgg tgttttgccc tgacgctcat cacgcagttg tttgacccat
     4741 cttgtcattg tagaaaggcc aacatccata gctttggcgg catctgccac cgtgtatttc
     4801 tggtcaacaa ccagttgagc ggattcgcgt ttaaactctg cgctaaaatt tctttttttc
     4861 attagagcac ctgtgttgtt ctgaggtgag catatcacct ctgttcaggt ggccaaattc
     4921 agtgtgccac ttcacagtga gcagctctgg tgaaggattt tccagcgtca tgttaccgtg
     4981 acggaactcc actttgcagg agctggcact gacagtagtc tgagtggata aggatggagt
     5041 aagagcagcc atcggttctt tcggctcatc aggcgttatc tctacaggta ataattcaac
     5101 gcctgcgtca gaagtggttg tcaccggaag acgccgtgat atacgccctt cgttttgcca
     5161 gagtctgagc catttgaaaa taacatcatc attgacgcca ttttctcgtg caatctgggc
     5221 catacaagca ccgggttgtg atgccagttc aaccatacga agtttgaatt cattcgaata
     5281 ctttttacga ggttcttttc gccagtcctg taattccata cttggatgtc cgtctgtgtc
     5341 agatgggcgt ctaagttacc aattcttgtc taatggctac atactgcggt cggtttacgc
     5401 ttacttttaa cgtaaaaacc gtgatggcaa gcctgtgccg tcacggactg agggcaaagg
     5461 cttcccggaa gttcagccct taccgcacac agcctgcctg tgtcagaaaa tctgctggag
     5521 ctgtcaacga cggatgaaaa gtgcctcttc cgcaacctgg gtggagattt tcatgccagt
     5581 ggactcatta ccctctgcct gagtagttca aatcctgctc gaccatacat ctggcgtttc
     5641 agcatcttca ggcgatttac atgaccttcg acaacaacat tactccagcg actactgatt
     5701 gcctcacata tagctgcagc atcagcttcc atccccgccg ccactcgccg aaattctgct
     5761 gaaccgcttt cgtggactcg agtgaaccaa ctacttagct gtgatttatt ttgggttttc
     5821 agaatacggt agaactcgag tgccagttgc tgcgctattt tcagctcagg ttcttttcac
     5881 acatcagact aataaatcgg gaagcataat tttcttcatc cctggttatt ctccagggca
     5941 tcaaccagcg gcttacccgg gccaccattt cccgccatat ccggctggcg ttataattac
     6001 cactttctcg ttgctctttt aaccactccc tccatgggtc cagaagccct cgctttggag
     6061 gtcgggttga catttcaggg aatgttcctg aacgcaccca gcgactgact gtcacacgcg
     6121 acaggcctgt aatacgggat atttccctga aactacatcc cttgttatgc tgggccataa
     6181 cctctgtcca acgctgatgc cgttttttgc gggtttgttg ttttaggcgt tccggacgac
     6241 ggagcgatgc tgcaggctca gacagttctg gctcaggtgg tttctttggt gacaactcgc
     6301 tggcaacaag acgtatcaga ggtatatgtc tgtacatcat tcgttcaagc gcatcgccaa
     6361 tattttttag caggtggcat cgaccacctg tctggcctgt ggtacacctt cacgtgctgc
     6421 tgtcgcacag accccactgc gatcacgcga gacaatctgt atttccggat attttctgaa
     6481 ccaggtcgcc agcgtacgct gatcacggcc gggaagcagg acgaggggac ggtgagtatc
     6541 aagattgacg attaacgtac cgtaacggtg gccccgatgc cacgcccact catcaatacc
     6601 gacatgaggc gcgcctgact gtttcgtctc cggggtattg ataaccctgc gaaggagagt
     6661 atcagcactg caacggagac ccactgccgt tgctgcccgt tttccggcct cacctccggc
     6721 gattaatccc agttgacgct gtaaattttg taacgcctgt gaagactgca gatgtgaacc
     6781 accgaaggga acaaacgact cggcaaaaat ttttcgtgaa caagccgggt tacggctgta
     6841 cctgtggcgg acggaaaata caagccatag cgtctgccct gagcagggta gatgctgtat
     6901 ccggcgccga cgacaactat gaacagagtc gctacgctta aagcattcag gacattgtgc
     6961 tgttttgcgt tttccgcgga gatggagggg tataccgtca gagttaataa tttgtcgact
     7021 acatcgccat ccgcaaggta actgtagaag tgttttaaga gagtgcattg cgttacccat
     7081 aactaaaaac agcttagatc cttgttcacc ataaagtttc atccattttc tccacccagg
     7141 ttgcggaaga accacttttg caccgttcgt gacaggagca ggatttttac gccagtagcc
     7201 agagccagaa gtgggcgcga gacatcacgt acttacgtac ggatgaaggc tggctgtatc
     7261 tgggagtggt taatgtcgcc acgtatgtct gcataagaga cctcggaacg ttatgctacc
     7321 gcatgtcatt tgcaaaaatg acagatagat atacttcaat ttaaacctta acaattagca
     7381 tgggaatgct gtcaatcctc tgtatgcaaa aatggcatct gaatcgctca ttatttagcc
     7441 ctctgtgtca aagcgtaaag tgttcccaga gagcctgtac atagattcgt gtaatttcct
     7501 gattttgata tgttcatcga caggaattta tcccccggaa agattaagaa aacaaaaaac
     7561 gaacatttat tcaacacatc cggaacctca tcgggtatat atcagcgctt atattgctga
     7621 cattgattga tacagggtat cggtgacgtt tttttgctcc atttccctca aaatggacgc
     7681 aacttcccct ctgcggctct cagccgcaat actgcattcg ggccagcggc tcatgcatca
     7741 taaccagctc tgccagacgg taaccccgct tcagccccgt aaaccgcatc tgactttcgc
     7801 acaggatgca tttcagcggg tcaaccttca gtaaccgctg atacatccct ctccacctga
     7861 tttgcatcgc cgtttttatc accgtctctg ttattacgta cactaattct tccagcaacc
     7921 ggcgtttcgc cggactaagg aacccgtaga acctcaccat acggaacccc ttatccgcca
     7981 catgccagga gaacctttcc ataaacgcat caccactcat caataggtat tcttcacgtc
     8041 gtgtgcggtg actgttgtaa cgcagtccga tttcatcctg accggcatag tgctccagac
     8101 ggctcattgg caccggcggc tttttcaggt aagagccaaa gtacaccgcc acatgtgtgg
     8161 cgttatccat cacctttgat acgttgacat tcctgccccg tcggtaatga ctgtccagga
     8221 aacgattcca ttcccgttta ctgcttcctt cagcagccag ctcatccggt atcaatagtt
     8281 ccgggtattt ccgtgacagt aaccgtgtta tccggtatcg ctacatactc atcaccttac
     8341 gggcgtaaaa atgaaggttt ttccaggtgt gatgtgatgt cacaccgccg gcagttgtcg
     8401 ataaatggat aaatggatat gtggatatgt ggatgacact gctggtcccg gccccatgtg
     8461 tggattaccg tgaatatccc cggcttcaca tctgctgcta tgcggctcat ctctgccagt
     8521 aaccaccggt tgtggaacac cagggaccag tactggcagg gaagcgtgaa tacaattgct
     8581 gccacgggcc gtcggggacc agactcagca gatactgtat ccactgtgca cccgctttca
     8641 ccccgcagtg tgggcaggag cgttttttac accgaaaaca gaccttcttt gtattgcaac
     8701 agtccgggca gtacatgccg ccggcgctga taccggcaag aatggtcgta aactcccgct
     8761 ctgtacagcg ggcgatttcc ggatgccctt cgtcatcaac acgtacaaac cagaagacca
     8821 gctttttgtt tccggcatcc acaaagaacg gaatattcag gtctgcgcaa cactcaacag
     8881 tatcgtcaaa attatcgaag cgcagaatct ctacttcttc ttcgtcaaaa aaatcatctt
     8941 cgtgaagttt tacaacatag cggggaagtt tgcttctttg aggtgcgagt tttcgtttac
     9001 ggggtttagc tgaacgtgcc atataactac ctgaaagaca atgacattgt ttgtttttat
     9061 aacggtaatt tcaggccatg acaagccgca gccgctaggc tgcct
//
LOCUS       DAZGOB010000165         9088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGOB010000165 DAZGOB010000000
VERSION     DAZGOB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.165"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1209
                     /locus_tag="ACHLIF_004470"
     CDS             <1..1209
                     /locus_tag="ACHLIF_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO9608347.1"
                     /translation="YLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLI
                     YLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNK
                     KKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLW
                     AFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKL
                     FVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDI
                     NQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKI
                     IAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSE
                     GGNKVIIHHI"
     gene            1390..2880
                     /gene="dgcJ"
                     /locus_tag="ACHLIF_004471"
     CDS             1390..2880
                     /gene="dgcJ"
                     /locus_tag="ACHLIF_004471"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO9608348.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2923..3426
                     /gene="yeaK"
                     /locus_tag="ACHLIF_004472"
     CDS             2923..3426
                     /gene="yeaK"
                     /locus_tag="ACHLIF_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO9608349.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3427..3531)
                     /gene="yoaI"
                     /locus_tag="ACHLIF_004473"
     CDS             complement(3427..3531)
                     /gene="yoaI"
                     /locus_tag="ACHLIF_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO9608350.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3701..4147
                     /gene="yeaL"
                     /locus_tag="ACHLIF_004474"
     CDS             3701..4147
                     /gene="yeaL"
                     /locus_tag="ACHLIF_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO9608351.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4104..4925)
                     /gene="nimR"
                     /locus_tag="ACHLIF_004475"
     CDS             complement(4104..4925)
                     /gene="nimR"
                     /locus_tag="ACHLIF_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO9608352.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5022..6203
                     /gene="nimT"
                     /locus_tag="ACHLIF_004476"
     CDS             5022..6203
                     /gene="nimT"
                     /locus_tag="ACHLIF_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO9608353.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRTLHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6258..6605
                     /gene="yeaO"
                     /locus_tag="ACHLIF_004477"
     CDS             6258..6605
                     /gene="yeaO"
                     /locus_tag="ACHLIF_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO9608354.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6627..6881)
                     /gene="yoaF"
                     /locus_tag="ACHLIF_004478"
     CDS             complement(6627..6881)
                     /gene="yoaF"
                     /locus_tag="ACHLIF_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO9608355.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7064..8089
                     /gene="dgcP"
                     /locus_tag="ACHLIF_004479"
     CDS             7064..8089
                     /gene="dgcP"
                     /locus_tag="ACHLIF_004479"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO9608356.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8122..8220
                     /gene="yoaK"
                     /locus_tag="ACHLIF_004480"
     CDS             8122..8220
                     /gene="yoaK"
                     /locus_tag="ACHLIF_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO9608357.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8223..8297
                     /gene="yoaJ"
                     /locus_tag="ACHLIF_004481"
     CDS             8223..8297
                     /gene="yoaJ"
                     /locus_tag="ACHLIF_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO9608358.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8356..8604)
                     /gene="yeaQ"
                     /locus_tag="ACHLIF_004482"
     CDS             complement(8356..8604)
                     /gene="yeaQ"
                     /locus_tag="ACHLIF_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO9608359.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8832..>9088)
                     /locus_tag="ACHLIF_004483"
     CDS             complement(8832..>9088)
                     /locus_tag="ACHLIF_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9608360.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         2247 a         2193 c         2127 g         2521 t
ORIGIN      
        1 tacctgatgg ttgttgtggc tttgtttttc cttaacgccg tcatttttct tttcatgtta
       61 atgaaatatt tcactaacaa acaaatttta ccaacactca ttttaagcct tgcattttta
      121 agtggcctta tctatttagt tgaaaccatt gtaattatcc ataaaccaat taacggcagt
      181 acactgatcc agacaaagtc gaatgatgtt tctattttct atattttccg ccaactcagt
      241 tttatttgtt taacctcgct ggcactcttt tgttatggaa aagacaacat ccttgacaac
      301 aataagaaaa aaacgggaat cctgttgctg gcgctgattc cttttttagt ttttcccctt
      361 ctggcacaca atctgagcag ttataacgct gactattctt tgtatgtcgt cgactactgt
      421 ccggacaacc atactgcgac ctggggaatc aactatacaa aaatattggt ttgtctgtgg
      481 gcatttttac tgttctttat tatcatgcgt acacgattag ccagcgaact atggccgtta
      541 atagcattat tatgtctggc atcgctatgc tgcaacttac ttctactgac tctggatgag
      601 tataattaca ccatctggta tatcagtcgc gggattgaag tttccagtaa actgtttgtt
      661 gtgtcttttc tgatttataa catttttcag gaactgcaac tctccagcaa actggcagtt
      721 catgatgtgc tgaccaatat ttataatcgg cgctactttt tcaacagcgt agagtcatta
      781 ttgtcgcgac ctgttgttaa ggacttctgt gtcatgctgg ttgatattaa tcagttcaaa
      841 cgcatcaatg cccaatgggg acatcgtgtg ggtgataaag tgctggtttc aattgtcgat
      901 attatccagc aaagcatccg ccctgatgat attttagcgc gactggaggg tgaggtgttt
      961 ggcttgctat ttaccgaact caatagtgcc caggcaaaaa tcattgcgga acgtatgcgt
     1021 aaaaatgtcg aactcctgac cggctttagt aacagatatg atgttcctga acaaatgacc
     1081 atcagtattg gcacggtttt ttcaacgggt gacacgcgta atatctcgct tgtcatgacg
     1141 gaagcagata aagccttacg cgaagcgaaa agcgaggggg gcaacaaagt gattattcat
     1201 catatttaag tgcaaaaata ttcagagcca tgctttttgc gtggcttttg catacaattt
     1261 attactatta cccctaaatt tcccctcccc agcggcgcag agatgagtat aattagcgcc
     1321 cctgtgccag gccgcaatcg aactttatct ggttttctcg tttcactaac cgaaggagtg
     1381 ccatttatca tgaaattgca ccatagaatg ctccggcatt ttatcgccgc aagtgtcatt
     1441 gtgctgacat cttccttcct tatttttgaa cttgtcgcca gcgacagagc aatgagtgcc
     1501 tatctgcgct atatcgtgca gaaagcagac tcctcctttc tttatgataa gtatcagaat
     1561 cagagtattg ccgcgcatgt gatgcgcgct ctcgctgctg agcagtcgga agtgtcgcca
     1621 gaacagcggc gcgccatctg cgaggctttt gagtctgcca ataataccca tggcttaaac
     1681 ctgactgccc ataaataccc gggcttacgc ggcacactac aaaccgcatc cactgactgc
     1741 gacacaattg tggaagctgc agcactatta cccgcttttg atcaggcagt ggaaggcaac
     1801 cgccaccagg atgattacgg ttcaggtctt gggatggccg aagagaaatt tcactattat
     1861 ctcgatctca atgaccgcta tgtctatttt tatgagccgg ttaatgttga atactttgcg
     1921 atgaataact ggtccttcct gcagtcagga agtattggca tcgatcgcaa agatattgaa
     1981 aaggtattta ccgggcgtac cgtattgtcg agcatttacc aggatcagcg tactaaacag
     2041 aacgtgatga gtttgctgac gccggtatat gtcgcagggc agctaaaagg gattgtgctg
     2101 ctggatatta acaaaaacaa tttgcggaat atcttttaca ctcatgaccg ccctctcctc
     2161 tggcgttttc tcaatgtcac gctaaccgat accgattcgg ggcgcgacat tatcatcaac
     2221 cagagcgaag ataatctgtt ccagtacgtc agttacgtcc atgacttacc gggcggcatt
     2281 cgtgtctcgt tatccattga tattctttac tttatcacgt cttcgtggaa aagcgttctg
     2341 ttctggattt tgacggcgtt aattttgctg aatatggtcc ggatgcactt ccgtttatac
     2401 caaaatgtgt cgcgagaaaa tattagtgat gcgatgactg gactgtataa tcgcaaaatt
     2461 ttaacccctg aactggagca gcggttgcag aaactggtgc aatccggttc ttcggtgatg
     2521 tttattgcaa ttgacatgga caagttaaag caaataaatg acaccctcgg tcatcaggag
     2581 ggggatttag cgattacgtt attagctcag gcgattaaac aatcgattcg taaaagtgat
     2641 tatgccatcc gactcggtgg cgatgaattc tgcatcattc ttgtcgattc gacgccgcaa
     2701 attgcagcac aactgcctga acgtatcgaa aaacgtctgc aacatatcgc gccgcagaaa
     2761 gaaatcggct tctcttccgg tatttacgcg atgaaagaaa acgatacgtt acatgatgcg
     2821 tataaagctt ccgatgagcg tttatatgtc aataagcaga acaaaaacag ccgttcatga
     2881 taaccttctg tggttgtttg cttgtaatct caggagcgtg aaatgactga aatggctaaa
     2941 ggaagcgtga cgcatcagcg gttaatcgct ttattatcac aagaaggtgc taacttccgc
     3001 gtcgttaccc atgaagcggt aggtaaatgt gaagcggtgt cagaaattcg tggcaccgct
     3061 ctaggccagg gtgctaaggc actggtatgt aaagtcaaag gcaatggcgt aaatcaacat
     3121 gttctggcga tcctcgctgc cgatcaacag gccgatctga gccaactcgc cagccatata
     3181 ggcggattac gcgcttcgtt ggccagcccg gcggaagtcg atgaactcac aggctgtgtc
     3241 ttcggcgcga tccccccttt cagcttccat ccaaaactca aactggttgc cgaccctcta
     3301 ctcttcgaac gatttgatga aatcgccttc aatgcaggga tgctggataa atccgttatt
     3361 ttgaaaacca cggattattt gcgcattgcg caaccagaac tgctcaattt ccgccgcact
     3421 gcgtaactag cctgtccgtt caataagcag aacggacaaa accagcacaa cagcaatggc
     3481 aaaaaacgat gacgtgataa tcagtgtctc gacgaacatt tgatcgttca tagcatgccc
     3541 ccgggagata gctgttttca cgttattgtt agcgtgcaca aatggcagtt tgatgacagt
     3601 tcgcctgatt ttttatgcaa aaaacgtaaa gattttctac ttccttcctg cagcaagcgt
     3661 aaagtaagca ggcttattat tttttggcaa ggaaaccacg atgtttgatg tcactttgct
     3721 gatcctgctc gggttagctg cgctgggctt tatcagtcat aacaccactg tcgccgtttc
     3781 aattctggtg ttgatcattg tccgcgtcac accgttaagc acctttttcc cgtggattga
     3841 aaaacagggg ctaagtatcg ggataatcat tctgacgatt ggcgttatgg cccccatcgc
     3901 cagcgggacg ctaccgccct caaccttgat tcactcgttt ctgaactgga aatcgctggt
     3961 ggcaattgca gtaggcgtta ttgtctcctg gctgggtggg cgcggcgtga ccttaatggg
     4021 cagccagccg caactggtcg ccgggctgct ggtaggcact gttttaggcg tggcgctgtt
     4081 tcgcggcgta ccggtcggcc cgcttattgc cgcgggtctg gtttcgctga ttgtggggaa
     4141 acagtagtta atcccgcaat atatcggcct ggcgtttgcc ccaggccttt cttaaacata
     4201 gtaataaagg ccgttgttga atcatacccc agcgtatgag caactttctg tacagtatcg
     4261 cctttcacta atccctgaag tgccataatc agctgtaact gctgacgcca ctggcgaaaa
     4321 ctcaaccccg tctcttttac tatcagtcgc gcaagattgc gttcactcat cgcaaaaaag
     4381 ccagcccatt gccctaatga cccccactcg acgggccctt tcgccatcat ctccaccatc
     4441 gtgcggattt taggatgaga agaaacgggc aggtgcagct gttgttgtgg ctgctgaggg
     4501 agttcatcaa acaggacctg aattaatctg cgggtcatcg gctcagcacg ctgagtcgtc
     4561 gtcctgtttg ccagggttaa aatcaattcc cggcataagg gcgatatttt caatgtgcaa
     4621 catgttgtcg gcattgtcac tgcgctgggt tcgataaaca gaaaacagag ttcagcgtta
     4681 gctgtcactt gattgctatg ttccaccccg cccggtatcc aaaccgcata ttgcggcggc
     4741 accatccaca aagcattttc caccgtacag gtaattgcac catgtaatgc tagtatcagc
     4801 tgtcctttgc ggtgctgatg tacaggactg gttaactcat ccgttccggc gtgaatacaa
     4861 aatgcgaccg cggcttcatg gtgtcggtcg ggttcatagc cattgagatt caacctgtgc
     4921 atcattttgt ccgaatttag cgataatttg tcattttagc ttgattcaac ataacaataa
     4981 aaacggtaag gtacagcctc gtttgtaaca atgagaagca tatgacctgt tcaacttcat
     5041 taagcggcaa aaacaggatt gtccttatcg ctggcattct gatgattgcc actacattac
     5101 gcgtcacctt caccggcgca gcaccgttac tcgatacgat tcgttccgct tactcgctga
     5161 cgacagcgca aaccggctta ttaaccaccc tgccattatt ggcctttgcg ctaatctcac
     5221 ctttggctgc cccggtggcg cgacgttttg gtatggaacg tagcctgttt gccgcgttac
     5281 ttttgatctg tgcaggtatc gcaattcgtt ctctcccttc gccttactta ttatttggcg
     5341 gtacagcggt cattggcggt gggattgcat taggcaatgt cttactgcca ggattaatta
     5401 aacgcgattt ccctcattcc gtcgccagac ttaccggcgc atattccctg acaatgggag
     5461 ctgcagcggc actgggatcg gctatggtcg tgccgctggc tttgaacggt tttggctggc
     5521 aaggcgcgtt gttcatgctg atgtgttttc ctctgctggc tcttttttta tggctgccac
     5581 agtggcgaag ccaacaacat gcaaatttga gtacctcgcg caccttacat actcggggta
     5641 tctggcgttc gccgcttgcc tggcaggtca cattgtttct tgggatcaac tcactggtct
     5701 attacgtgat tattggctgg cttccggcga tcctcatcag tcgcggctat agcgaagcac
     5761 aggcgggttc actgcatggt ttgctgcaac tagccacagc agctcccggt ttgctgatcc
     5821 ctcttttctt acatcatgtg aaagatcagc gtggtattgc agcgttcgtt gccttgatgt
     5881 gcgcagtggg cgctgttggg ctctgcttta tgccagcgca cgcgatcacc tggactctgc
     5941 ttttcggttt tggttccggc gcaacaatga tactggggtt gacgttcatt ggtcttcggg
     6001 caagttctgc gcatcaggcg gcggcactct cggggatggc acaatccgtc gggtatttgc
     6061 tggcagcctg tgggccgccg ctgatgggta aaatacacga tgctaacggt gactggtccg
     6121 taccactgat gggtgttgcc atactttcac tactgatggc gattttcgga ctttgcgccg
     6181 ggagagacaa agaaattcgc taatatccgg tgctatagtg acgtgacaaa tcatgcgtga
     6241 aagggagaac aaacacgatg aatattcagt gcaaacgcgt ttatgatccg gctgaacaga
     6301 gcgatggtta tcgcgtactg gtcgaccgcc tctggccgcg cggtatcaaa aaaaccgatt
     6361 tagcccttga tgagtgggat aaagaaatca cgccgtcaac ggaactgcgc aaagcctttc
     6421 acggcgaagt cgtcgatttt gcaacctttc gcgagcaata tcttgcagaa ctggcgcaac
     6481 acgagcaaga aggaaagcgg ctggcggaca tcgccaaaaa acagccgctg accctgctct
     6541 acgcagcaaa aaacaccacg cagaaccatg cgctggtgct ggccgactgg ctacgtagct
     6601 tgtgatttta gtacagcatc cggcggttat ttttcaccag ccggatggtc ccgtcgccac
     6661 aatgcccatt catcaatcgt ttcaccgccc ggtaatttgc aattgttgct gaccccttgc
     6721 gctgtctgca ctggaatgag cgtcccgccc ttctgctggc aatagaccga cgccggattt
     6781 gccataccaa tctgcggcgg tttaggtgct tctggctgag aagaggttga acaaccagcc
     6841 aggaccagca agcaaggcaa agcaaaactg ataattttca tttattgatc tcacacattt
     6901 atctacgctt agagtatcgc ggtatcgttt tgttttgcag cactattttt attacattca
     6961 ctcaaaacat attacgtctt gtttcatctt tgttgatgat gttttatcat gcctgcaaag
     7021 attaaataat cagcatttac ccgccgtatc ctggagttgt ttcgtgtcag atcagattat
     7081 cgcccgcgtc tcgcaatccc ttgccaaaga acagtcacta gaaagtctgg tccgacagct
     7141 tctggagatg ctggaaatgg tcactgatat ggaatcaacc tacctgacca aagtggatgt
     7201 cgaagcgcgc ctgcagcata ttatgtttgc ccgtaacagc cagaaaatgc acatcccgga
     7261 gaattttacc gtctcgtggg attactcgtt atgcaaacgc gctattgatg aaaactgctt
     7321 tttcagcgat gaagtccctg accgttgggg cgactgtatt gcggcacgca atcttggaat
     7381 caccacattt ctgagtacac caattcactt accggatgga tcattctatg gcacgctttg
     7441 cgccgccagc agtgagaagc gccagtggag tgaacgcgcg gaacaggttt tacagttatt
     7501 cgccggactg attgcacaat atattcaaaa agaggcgttg gttgaacagc tgcgcgaagc
     7561 caatgccgcg ctgattgcgc aatcgtatac cgactcgtta accgggctac cgaatcggcg
     7621 ggcgattttt gaaaatctga cgacgctgtt ttctctcgcc cggcatctta accataagat
     7681 aatgatcgcg tttatcgatc tggataactt caaattaatc aatgatcgtt ttggtcataa
     7741 tagtggcgat ctgtttctca ttcaggttgg cgagcgcctt aatacgctcc agcaaaatgg
     7801 cgaagttatt ggtcgtctcg gcggtgatga gtttttggtt gtttcactaa ataacgagaa
     7861 tgcggatatt tcgtcgctgc gagaacgtat tcaacagcaa atacgtggag aatatcactt
     7921 aggtgatgtt gatttgtatt atcctggtgc cagtcttggc attgtagaag tcgatcctga
     7981 aacgaccgat gcagacagtg ccctgcatgc tgccgatatc gcgatgtatc aggagaaaaa
     8041 acacaaacag aaaacacctt ttgtcgcgca ttcagcgcca cattcctgag gcgtattcac
     8101 atccttttga ttggtgataa catgcgaatc ggtattattt ttccggttgt aatctttatt
     8161 acagcggtcg tatttttagc atggtttttt attggcggct atgctgcccc gggagcataa
     8221 agatgaaaaa aacaacgatt attatgatgg gtgtggcgat tattgtcgta ctcggcactg
     8281 agctgggatg gtggtaacgt cacctctaaa aaatagcaaa ggctgcctgt gtgcagcctt
     8341 tgtgcaattt aagcgttaac ttttaatctt cctgtagata aatagcacga caatcgcacc
     8401 aataacggca accacgaagc tgccaaaatt gaagccatcg actttaccaa agccaaacag
     8461 cgtgctgatc catccgccaa ctacggcacc gactatcccc agcaggatag tcataaagaa
     8521 tccaccacca tctttacctg gcatgatcca cttcgccaga ataccggcaa taagcccaaa
     8581 aataatccat gacagaatgc ccattgtttc ctcacttatc tgttttgcat tagcgggtta
     8641 gtcgctgata aaaagcatag cacaacatcg ggagggcaag atttgtgacg agcatcacgg
     8701 aggatttttt tgcggatggc acagaaattg cgccatcaac gatcagtgat aattaccaac
     8761 cacaaacatc atgttcgttt tccgtgtctt gacatcctcc acgccctgaa tgacgaggat
     8821 ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgggcgg
     8881 cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga
     8941 gcgccgttta catcggcgtt cgctgtatat ccacatacct ggcatctgaa ttgactttgt
     9001 gacaggcgat tttctttcgc tgtatgacca cagcacgcgc aacgctggct tgtgtacgct
     9061 ggcggaacag caagcacctg accgccac
//
LOCUS       DAZGOB010000166         8753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGOB010000166 DAZGOB010000000
VERSION     DAZGOB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.166"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            123..5300
                     /locus_tag="ACHLIF_004484"
                     /pseudo
     CDS             123..5300
                     /locus_tag="ACHLIF_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5523..5789
                     /gene="hipB"
                     /locus_tag="ACHLIF_004485"
     CDS             5523..5789
                     /gene="hipB"
                     /locus_tag="ACHLIF_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO9608361.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5789..7111
                     /gene="hipA"
                     /locus_tag="ACHLIF_004486"
     CDS             5789..7111
                     /gene="hipA"
                     /locus_tag="ACHLIF_004486"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO9608362.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7423..7631
                     /locus_tag="ACHLIF_004487"
                     /pseudo
     CDS             7423..7631
                     /locus_tag="ACHLIF_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8063..8626
                     /gene="fimA"
                     /locus_tag="ACHLIF_004488"
     CDS             8063..8626
                     /gene="fimA"
                     /locus_tag="ACHLIF_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO9608363.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT         2397 a         1849 c         2339 g         2168 t
ORIGIN      
        1 tgacaccttc ccacctccaa ctaacactca atacagacct ctgacaattt aataagtttc
       61 atttttaatg tctgacgact catctctttc gtggctgtat tttttgtata tggagcgttt
      121 atatgaatag aatctatcgc gtgatatgga attgcactct acaggtattt caggcctgct
      181 cggaattaac tcgcagggca ggtaaaacat cgacggttaa tttgcgtaaa tcctctggac
      241 tgacaacgaa attcagtaga ttgacgctgg gtgttttgct ggcactaagc ggttcagcgt
      301 ctggtgcaag tctggaagtt gataatgatc agattaccaa tattgatact gatgttgctt
      361 atgatgccta cctggttggc tggtatggca ctggagtgct taatattttg gctggcggta
      421 atgcctcctt aaccactatt actaccagcg tcattggcgc taatgaggac tcagagggca
      481 ccgttaatgt tttgggtggc acctggcgat tgtatgatag cggaaataat gcaaggcctt
      541 taaatgtggg tcaatccgga acggggacgc tgaatattaa acagaagggt cacgtcgatg
      601 gaggctattt aagattaggt tcttcgacag gaggcgtcgg gacggtcaat gttgagggag
      661 aggactctgt tctgacgacc gaattattcg aaatagggag ttacggtacg ggctcattaa
      721 atattacgga taagggttac gtcacgagtt caatcgtcgc cattttaggc tatcaggcgg
      781 gcagtaatgg ccaggttgtc gttgaaaagg gtggcgagtg gctaataaaa aataacgatt
      841 cctcaattga atttcaaatt ggtaatcaag gaactgggga ggcgactatt cgcgagggtg
      901 gactgattac ggctgaaaat acgattatcg gtggcaatgc caccggtgtc ggaaccctga
      961 atgtgcagga tcaagactct gtcatcacgg tacgcagact ctataatgga tatttcggta
     1021 atggcacact caatatttcc aataatggac taattaataa caaagaatat tcattagtgg
     1081 gcgttcagga cggttcccac ggtgtcgtca acgtgaccga taaagggcat tggaatttcc
     1141 tcggaacggg cgaagctttc cgctatatct atatcggtga tgctggcgac ggtgaactta
     1201 atgtctcgcg tgaaggtaaa gtagattcgg gaattatcac tgcggggatg aaagaaacag
     1261 gcacaggcaa cattactgtt aaggataaga actccgttat cactaatctc ggaactaatc
     1321 ttggttatga cggccacggc gaaatgaata tcagtaatga agggcttgtt gtcagcaacg
     1381 gaggaagctc actcggttat ggagaaaccg gcgtcgggaa tgtcagcatc accacggggg
     1441 ggatgtggga ggtcaataag aatgtctata ccaccattgg tgttgcgggc gtcggaaacc
     1501 tcaatattag cgatggcggt aagttcgtat cgcaaaatat tacttttttg ggcgataaag
     1561 caagcggtat cggcacactg aacctgatgg atgcgacatc atcgttcgat actgtgggta
     1621 tcaatgtcgg taattttggt agcggtatcg taaatgtcag taatggtgcc acccttaatt
     1681 caacgggcta tggatttatc ggaggaaatg cctccggtaa ggggatagtt aatatttcaa
     1741 cggacagtct ctggaattta aagacatcat ctactaacgc acaattgcta caggtcggtg
     1801 tattaggcaa gggtgaactg aatattacca ccggaggtat agttaaagcg cgtgatacac
     1861 agatagctct caatgacaaa agtaagggcg acgtgagggt ggatgggcag aactctcttc
     1921 ttgaaacatt caatatgtac gtagggacat ctggtacggg tacgttaacc ctgacgaata
     1981 gcggcacgct gaatgtcgaa ggtggagaag tttacttagg tgtttttgaa cctgctgtag
     2041 gaacgctaaa cattggtgca gctcacggtg aggcggcggc agatgccggg tatataacca
     2101 acgcgacgaa agtagagttt ggttctggcg aaggtgtttt tgtctttaat catactaata
     2161 acagtgatgc cggctaccag gtcgatatgc tgattacagg tgacgataaa gacggaaaag
     2221 tgatccatga tgcaggccat acggtgttca atgcagggaa tacttatagc ggtaaaacgc
     2281 tggtcaatga cggcctcctg accattgcgt ctcatacggc agatggggta acgggcatgg
     2341 gttcgagtga agtcaccatt gcaagccccg gtacgctcga cattctcgca tcaacgaaca
     2401 gtgcaggaga ttacacgctg accaatgcgc tcaaaggcga tggcttgatg cgagtgcagc
     2461 tgtcatccta cgacaagatg tttggcttta cccatgcaac agggactgaa ttcgccggtg
     2521 ttgcccaact gaaagacagt accttcactc tggaacgcga caacaccgct gcgcttactc
     2581 acgcgatgtt gcagtctgac agtgaaaata ccacatcggt aaaagtggga gagcaatcca
     2641 ttggtggact ggccatgaat ggcggtacgc tcattttcga tacggatatt cctgctgcga
     2701 cgcttgcaga gggatatatc agcgtcgata cgctggttgt cggcgcgggt gactacacct
     2761 ggaaaggccg taactatcag gtaaacggga cgggcgacgt gcctaaaccg tggaatgatc
     2821 ccatggcgaa taaccctctg acgacgctca atttgctgga acacgatgat agccatgtcg
     2881 gcgttcaact ggtgaaggcg caaacggtta ttgggtcggg tggctcatta acgttacgtg
     2941 atttacaggg cgacgaggtg gaagcggaca aaacgttaca cattgcgcaa aacggaacgg
     3001 tggtcgccga gggtgattat ggattccgcc tcacgaccgc accaggtgat ggtttgtacg
     3061 ttaactatgg gctgaaagcg ctgaacatcc atggtgggca aaagctgacg ttagccgaac
     3121 atggcggagc ctatggcgca acggccgata tgtcggcaaa aatcggtggt gaaggggatc
     3181 tggcaatcaa tacggtgcga caggtttcgc tttccaacgg tcagaacgac tatcaggggg
     3241 caacctacgt tcagatgggg acattacgta ccgatgcgga tggtgcactt ggcaacaccc
     3301 gggaactgaa catcagcaac gcggccatcg tcgatcttaa tggatcgacg cagacggtag
     3361 agacattcac cgggcagatg ggttcgactg ttttgttcaa agagggggcg ctgacggtaa
     3421 ataaaggtgg gatcagtcag ggtgaactga caggtggcgg aaacctgaat gttacagggg
     3481 gaacgctggc tatcgagggg cttaatgcac gctacaatgc gttaaccagc attagcccaa
     3541 atgcggaagt cagcctcgat aatacgcagg ggttaggcag aggaaatatt gccaataacg
     3601 gtctgttaac gctaaaaaac gtgactggcg aactgcgcaa tagcataagc gggaagggta
     3661 tcgtgagcgc aaccgccagg acagatgtag agctggatgg cgataatagc cgctttgtgg
     3721 ggcaattcaa cattgataca ggcagcgcgc tcagcgtcaa cgagcagaaa aacctgggtg
     3781 atgcttccgt tatcaataat ggcctgctca ccatctccac tgagcgtagc tgggcgatga
     3841 cgcacagtat cagcggtagc ggtgatgtga caaaactggg taccgggatc ctgactctta
     3901 acaacgattc cgcggcgtat cagggtacga cggatatcgt gggggggggg aaattgcttt
     3961 cggttccgac tctgccatta atatggcaag tcaacacatt aatatccata acagcggtgt
     4021 gatgtcggga aatgtcacca ctgcaggtga tgtgaacgtt atgcctgggg ggacactgcg
     4081 tgtcgctaaa accacaatcg gcggcaacct ggagaatggc ggcacggttc aaatgaatag
     4141 cgaagggggg aaaccgggga atgtactgac cgttaacggc aactataccg gaaacaatgg
     4201 cctgatgacg ttcaacgcga cgctgggcgg cgataattcg cccaccgata aaatgaacgt
     4261 gaaaggcgat acccaaggga acactcgcgt tcgggttgat aacattggcg gcgtcggtgc
     4321 acaaacggtc aacggtattg aactcattga ggttggcggt aattctgcag gtaacttcgc
     4381 gctgaccacc ggaactgtcg aagctggggc ttacgtctac acgctggcta aagggaaggg
     4441 gaatgacgag aaaaactggt atctgaccag taaatgggac ggcgtaacgc cagcggatac
     4501 acccgatccc atcaataatc cccctgttgt ggatccggaa ggcccatcag tttatcgccc
     4561 ggaggccgga agctatatca gcaacattgc cgcagccaac tcgctgttta gccatcgttt
     4621 acacgaccgt ctgggtgagc cgcagtatat agattcactg cattctcagg ggtcggcaag
     4681 cagtatgtgg atgcgtcatg tcggagggca cgaacgttca agggccggtg acggtcagct
     4741 aattactcag gctaaccgct atgtattgca gctaggcggc gatttggcgc agtggagtag
     4801 caacgcgcag gatcgctggc atgacggcct ctgttgaggg tgggtatacc tttgaagcgg
     4861 gaacatttag aggcagcgaa gggacgctga atacctggta cgtccagcca caggcgcaaa
     4921 tcacctggat gggtgtgaaa gattctgacc ataatcggaa agacggaaca cgcattgaaa
     4981 cggaaggcga cggaaacgtg caaacgcgac ttggggtgaa aacctacctg aatagccatc
     5041 acaagcgtga cgatggtaaa cagcgtgagt tccagcctta cattgaagcg aactggatca
     5101 acaatagcaa agtctacgcc gtgaagatga atggtcaaac cgtaagccgt gatggtgcgc
     5161 gtaatctcgg tgaagtacgt accggggttg aggcgaaagt aaataacaac cttagcctgt
     5221 gggggaatgt cggtgtgcaa ctaggtgata aaggctatag cgatactcag ggcatgctgg
     5281 gagtgaaata tagctggtaa accgtataag ccgcatgtcg agatggcatg cggcttaata
     5341 ttgccgactt caaacggcgc atcagcgcct tttttaaatc ctccttttta tccgcgatcg
     5401 cggatatcgc agcgtttatc ccgtagagcg gataagacgt gtttccaaat tgacttatcc
     5461 tcactaaagg ataaaactga taatatcctc ttatgcggat aaatttgctg tggacgtatg
     5521 acatgatgag ctttcagaag atctatagcc caacgcaatt ggcgaatgca atgaaactgg
     5581 ttcgccagca aaatggctgg acgcagagcg agctggcgaa aaaaattggc attaagcagg
     5641 cgacaatttc caatttcgaa aacaaccctg acaataccac gctcacgaca ttttttaaga
     5701 ttttacagtc gcttgaactc tcaatgacac tatgcgacac gaaaaatgcc tcgccagaat
     5761 caacagaaca gcaagatctg gagtggtaat gcctaaactt gtcacttgga tgaacaacca
     5821 gcgggtaggc gagttaacga agttagccaa cggcgcgcac acctttaagt atgcaccgga
     5881 gtggttagcc agccgttatg ccagaccgtt gtcactttcg ctgccattgc agagggggaa
     5941 tatcacctca gatgccgtat ttaacttctt cgataacctg ttacccgata gcccgattgt
     6001 acgtgaccgg atcgttaaac gttatcatgc taaatccaga caaccgtttg atttattgtc
     6061 agaaataggg cgagacagcg ttggtgccgt aacgttatta cccgaaaatg aaaccataac
     6121 gcgcccgata atggcatggg aaaagcttac tgaagccagg cttgaagaag tattaacggc
     6181 ttataaagca gatatccctc tgggcatgat tagagaagaa aatgactttc gcatctcggt
     6241 tgctggcgcg caggagaaga cagcgctgct tagaataggc aatgactggt gcattccgaa
     6301 aggaataacg ccgacgacgc acatcattaa attaccgatt ggcgaaatca gacagcccaa
     6361 tgcgacgctc gatctcagcc aaagcgttga taatgagtat tactgtctgc tgctggcgaa
     6421 agaacttggg ttgaatgttc cggacgcaga aatcattaaa gcgggaaatg tgcgcgcgtt
     6481 agcggtcgaa cgttttgaca ggcgttggaa tactgagcga acggttttac ttcgcttgcc
     6541 acaggaggat atgtgtcaga cattcggttt accttcatct gtgaaatatg aatcagatgg
     6601 aggcccaggc atcgcgcgga tcatggcgtt tttgatgggg tccagcgagg cgcttcgcga
     6661 tcgttatgat tttatgaaat tccaggtctt ccagtggttg attggcgcaa cggatggcca
     6721 tgcaaaaaac ttctccgtat ttattcaggc tggcggcagt tatcgactca cgccatttta
     6781 cgacatcatt tcagcatttc cggtccttgg cggtacggga atacacatca gcgatctcaa
     6841 actggcaatg gggcttaacg catccaaagg caaaaaaacg gcaatcgata aaatttatcc
     6901 gcgacatttt ttggcgacag caaaggtgct gagattcccg gaagtgcaga tgcatgaaat
     6961 cctaagtgac tttgccagaa tgattccggc agcactggat aacgtgaaga cttcattacc
     7021 gacagatttt ccggagaacg tggtgacggc agttgaaacc aatgtgttga ggttgcatgg
     7081 acggttaagc cgagaatacg gtagtaagtg agatatgggg tttggtcgtt gttaatgagc
     7141 acgacaatca tgaccgccaa cgactggatt cttccttggt ttatactggt gcgcataatg
     7201 tatattatgt taaattatgt ctgagtgatg cgaagtgctt ttctggtagt cgttattcgt
     7261 tcagggttca agaacttaag cctctcctct ttcataattg gcctgtgcat ggagaaatag
     7321 cctggttatt ccctgcccca tgctaagagc gcagtgtctg tcgcatagac actgcatacg
     7381 aaaacaccag agggtgctgt tacttattta aggacggatc aagcgggaat gagtccactc
     7441 cggtatcaga aatggctacg tctcaatgaa gtcaggcgac cgatgctgaa tgaacattac
     7501 gatgtcacca cagcagccta tgctgtcggt tacgaaagct atcccatttc agtcgggaat
     7561 attcgcggat gtttggagag tcacccaaga gagatattac caggttaaga aagtctgtcg
     7621 gtcagcttta ataaaccctg cacttatctg tttagagcaa ttcggtgtta gtttcagcaa
     7681 gcaaacatta accatagcta atgatttata gccatattaa ccattggtag cccttgacct
     7741 ttcctgacac ttcccttgtt cgcaataata ttctgtttag tcacggttta taaataacat
     7801 gcataatgaa atcgtcatgt tatttagcga cataaaatgt ttttgcaaat actctcttca
     7861 acaagtgcac aataaacttg ttgtgattag gtgtccttgt gatttaaaga tattatcgtt
     7921 cttcgtgaaa tgtaataacc ctggaaacta tattctcagg gcaacaacca atcaagaatg
     7981 attcttgttt ttaagcaata acagaatgac ttaattgtta accctcttta ttcagctact
     8041 taaatataaa ttttggagaa ttatgaaact caaacatgtt ggtatgattg tcgtttctgt
     8101 gttggcgatg tcgtctgctg cggtaagcgc agccgagggt gatgaatcag taacgaccac
     8161 tgttaatggc ggtgttattc attttaaagg tgaagtggta aatgccgctt gtgcaattga
     8221 ttcagaatca atgaaccaaa cggttgagct gggtcaggtt cgttcttctc gcttggctaa
     8281 agcgggtgac ctcagctccg ccgttggctt caatatcaag ctgaatgatt gtgataccaa
     8341 tgtttccagt aatgcagctg ttgcattcct gggtactact gtcaccagta atgacgatac
     8401 gttagcgctg cagagttcag cggcaggctc tgcccaaaat gtcggtattc aaattttgga
     8461 ccgtacgggt gaggtattaa tacttgatgg ggccactttt agtgctaaaa ccgacttgat
     8521 tgatggcacg aatatactgc cattccaggc tcgttatatt gctctcgggc agtccgtagc
     8581 tggtactgca aacgctgatg cgaccttcaa agttcaatat ctataattca atcttaaggt
     8641 ggctatacgt catctgaaat tgaaacaggg tgtgtgtggc atggctacgc acacccgagc
     8701 aatttaccaa tattcatctc atttttaatg agaaatggtt tgtatttgga gcg
//
LOCUS       DAZGOB010000167         8710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGOB010000167 DAZGOB010000000
VERSION     DAZGOB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.167"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..555
                     /locus_tag="ACHLIF_004489"
     CDS             <1..555
                     /locus_tag="ACHLIF_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO9608364.1"
                     /translation="DNKFDDFMRQHRFSKVDFDTQQMIHFINRFNMNKGLIDKNNNFF
                     IQLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPLFIEIEDNLRTFNTNELTRKDNIF
                     ILFSSADDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKS
                     HGKYALFPIFTASWRATRIKNIGI"
     gene            778..1275
                     /gene="ubiC"
                     /locus_tag="ACHLIF_004490"
     CDS             778..1275
                     /gene="ubiC"
                     /locus_tag="ACHLIF_004490"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO9608365.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1288..2160
                     /gene="ubiA"
                     /locus_tag="ACHLIF_004491"
     CDS             1288..2160
                     /gene="ubiA"
                     /locus_tag="ACHLIF_004491"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO9608366.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2315..4738)
                     /gene="plsB"
                     /locus_tag="ACHLIF_004492"
     CDS             complement(2315..4738)
                     /gene="plsB"
                     /locus_tag="ACHLIF_004492"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO9608367.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4909..5277
                     /gene="dgkA"
                     /locus_tag="ACHLIF_004493"
     CDS             4909..5277
                     /gene="dgkA"
                     /locus_tag="ACHLIF_004493"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO9608368.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5387..5995
                     /gene="lexA"
                     /locus_tag="ACHLIF_004494"
     CDS             5387..5995
                     /gene="lexA"
                     /locus_tag="ACHLIF_004494"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO9608369.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6068..7393
                     /gene="dinF"
                     /locus_tag="ACHLIF_004495"
     CDS             6068..7393
                     /gene="dinF"
                     /locus_tag="ACHLIF_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO9608370.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7509..7718
                     /gene="yjbJ"
                     /locus_tag="ACHLIF_004496"
     CDS             7509..7718
                     /gene="yjbJ"
                     /locus_tag="ACHLIF_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO9608371.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7760..8275)
                     /gene="zur"
                     /locus_tag="ACHLIF_004497"
     CDS             complement(7760..8275)
                     /gene="zur"
                     /locus_tag="ACHLIF_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO9608372.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8593..>8710
                     /gene="yjbL"
                     /locus_tag="ACHLIF_004498"
     CDS             8593..>8710
                     /gene="yjbL"
                     /locus_tag="ACHLIF_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO9608373.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKG"
BASE COUNT         2046 a         2072 c         2338 g         2254 t
ORIGIN      
        1 gataacaagt tcgatgattt tatgcgtcaa catcgattca gcaaggtgga tttcgatacc
       61 caacagatga tccattttat taacaggttt aatatgaata aagggctgat tgataaaaat
      121 aacaattttt ttatccaact tatcgatcag gccttacgat caacggatga tatgatcaaa
      181 gcaaatgcct ggtatcttta taaagagtgg attcgtagtg atgatgtttc acctctattt
      241 atagaaattg aagataattt aagaaccttt aacacgaatg aattaacacg aaaggataat
      301 atctttatcc tgttctcctc tgccgatgat gggccagtta tggtggtaag ctcccagcgc
      361 ttacatgata tgttgaatcc tacaaaagat accaattgga attccacgtg tatctataaa
      421 tccagacata agatgttgcc tattaatctt actcaggaaa cactttttag ctccaaatct
      481 catggtaaat atgcgctttt cccaattttt actgcgagtt ggcgagctac tcgtataaag
      541 aatataggta tttaagtaaa ggaaaacatc accgttcctg gcatcatggg cggtgatgcc
      601 cctacggttg ccctcgccag cacgggcatc ggcaaagcgt aaggttcaac agcggtttac
      661 cacttcatgc gattgttgcg tttttgttgc gcattagatc acttaatttg ctttacatct
      721 cccgtaaaca cttttctgcg atacaatgcc tttacgttat gtaacggaga gttcggcatg
      781 tcacaccccg cgttaacgca actgcgtgcg ctgcgctatt ttaaagagat ccctgccctg
      841 gatccgcaac tgctcgactg gctgttgctg gaggattcca tgacaaaacg ttttgaacag
      901 cagggaaaaa cggtaagcgt gacgatgatc cgcgaagggt ttgtcgagca gaatgaaatc
      961 cccgaagaac tgccgctgct gccgaaagag tctcgttact ggttacgtga aattttgtta
     1021 tgtgccgatg gtgaaccgtg gcttgccggt cgtaccgtcg ttcctgtgtc aacgttaagc
     1081 gggccggagc tggcgttaca aaaattgggt aaaacgccgt taggacgcta tctgttcaca
     1141 tcatcgacat taacccggga ctttattgag ataggccgtg atgccggact gtgggggcga
     1201 cgttcccgcc tgcgattaag cggtaaaccg ctgttgctaa cagaactgtt tttaccggcg
     1261 tcaccgttgt actaagagga aaaaaatatg gagtggagtc tgacgcagaa taagctgctg
     1321 gcgtttcatc gcttaatgcg tacggataag ccaattggcg cgttactgct gctctggcca
     1381 acattatggg cgttgtgggt ggcgacaccg ggcgttcctc agctctggat cctggcggta
     1441 tttgtcgcgg gtgtctggct gatgcgcgct gctggatgtg tggtgaatga ttatgctgac
     1501 cgaaagtttg atggtcatgt taagcgcacg gcgaaccgac cacttcccag cggcgcggta
     1561 acagagaaag aggcgcgcgc actgtttgta gtgctggtac tgatttcgtt tttactggta
     1621 ctgacgctga atacgatgac cattctgttg tcgattgccg ctctggcgtt ggcgtgggtg
     1681 tacccgttta tgaagcggta tacccatctc ccgcaagtgg tgctgggcgc ggcgtttggc
     1741 tggtcgattc caatggcttt tgccgctgtg agtgagtcgg tgccattgag ttgctggtta
     1801 atgttcctcg ccaatattct ctgggcggtg gcttacgaca cgcagtatgc gatggttgac
     1861 cgcgatgatg atgtgaagat tggcattaaa tccacggcaa tcctgttcgg ccaatacgat
     1921 aaattgatta ttggtatttt gcagattggc gtactggcac tgatggcgat catcggtgag
     1981 ttaaatggct taggctgggg atattactgg tcaattgtgg tggctggcgc gctgtttgtt
     2041 tatcaacaaa aactgattgc caaccgcgag cgtgaagcct gctttaaagc atttatgaat
     2101 aataactatg ttggtctggt actattttta gggctggcaa tgagttactg gcatttctga
     2161 tgatgtaaaa aagccggatg atcatccggc tttcttctgg gttgcctgat gcgcggcgct
     2221 tctcaggcct acacaacaca tcgcaattta ttgaatttgc agattatgga aggccggata
     2281 aggcgttttc gccgcatccg gcaattctct ctgattaccc ttcgccctgc gtcgcactct
     2341 caatcgtcaa acgcacgtct gatgtaatca actccgccag caactgataa accttcatcg
     2401 tttctgccgg ttcggcatcg ccgctatcgc tgatataccc ttcatcacgc agtgtcagca
     2461 ccagagaact gaacaccgcc ttgtcgaaga actccggcgc gttgatgccg tgcagcacgg
     2521 agagacgttg cgcgacggtg cggctctctt tctccagcgt accgcggttg atcgacgggt
     2581 tggcactcaa caaccagaag gtgatggcat aacgttgcag cgtttcgcgc gcgcctgcgg
     2641 cgagcagctg tagcgtgcga gaatgcaccg ggttgatatg caactcatca tcttgcaggg
     2701 taatcagccc ctgacgttgc atctcatttg ccagcgcatc aataacgtcc ggcaactcgt
     2761 cgcgatccca gcgcaggaac agctccgctt tcagcattgg gtaaagcaca ttgacgtgct
     2821 ccatcaatac gtcgcgggag atgtggcgat gctgggtgac aattgccgcc atcagcgaag
     2881 gcagcaccaa catatgcgca atgttgttgc gatagtaggt catcagcacc gcttgctcgc
     2941 gtggcagaat gatgatgtcg ccgatagtgt ctttctcgac ttcaaacttg ttcatttgca
     3001 gcgcgtgatc gataagctcg ctggcgctgg ctgaaggaac ggtagagtcc gtggagtaag
     3061 gtacgttgcg catcagatcc aggtagcagt tgagttgctc ggttaactgc tcgcgggtga
     3121 gtgagcgctg acgtgatgcc agtagcgcag tacagcacag gttcatggcg tttgccgcgc
     3181 ctgcgttgtt aatgcgtacc atcagatcgg cagcaatatt attgaccgtc ggcgttaacc
     3241 aggccggacg caccgcttcg atgggatcga tagattcacg ccagtctggt acgtgctggt
     3301 taaggtaggt catcaacggc attggttcac cgaagttgac gtaaccctga ccgagattac
     3361 gcagcttgct taaaccgcgc agcatctgcg gcaggctctc tttctctttc gtcgcgccgc
     3421 gcagttcttt ggcgtaagta cccacttcca tgacgtgctc ataaccgata tagatcggaa
     3481 tcagcgtaat cggacgcgtg ccgccacgca gcatcgcctg aatggtcatc gacagcgtac
     3541 cggttttcgg atccagcaaa cgccccgtgc gggaacgacc gccttccacg aagtactcga
     3601 cggaataacc acggctgaac agttcgccga gatactcacg gaaaacggtg gaataaagtt
     3661 tattgccttt aaaagtacgg cgaataaaga acgcccccag acggcggaaa atcggcccgg
     3721 ccggccagaa gttcaggttg atcccggcgg cgatatgcgg cggcaccagc ccctggtggt
     3781 acagcacgta agaaagtagc aggtagtcca tgtgactgcg gtggcaaggc acatacacca
     3841 gctcatgacc gtcgtgggcc agctggcgaa cgcgctcggc gttatggacg ttgatgccct
     3901 gataaagtcg gttccaggtg aagcccagaa tgcggtcagt caggcggatc atctcgtaag
     3961 agaaattcgc cgcaatctct tccatcagcg caatcgcgtt ctgctgcgct ttttcatggg
     4021 agattttttt gctgcgcgct tcatcttcta ccgctttggc aatggcgcgg gaggcgagca
     4081 gcttattaaa cagatcctga cgagcaggca gacgtgggcc cacagcagcc agacgttgac
     4141 gggcaaagtg catacgcgcc acgcgcgcca gtttctgagc gatagtttta tctgtgccgt
     4201 gttcatcggc catgcggcgc agagaaactg acggcgagaa acgcacaaaa ctgtcgcgac
     4261 cgagccacag tacggcgaaa aatttttgta cgccgttaag catacgcagc ggcgggttta
     4321 cttcgccttt ttcacgcccc ggcgcgcgac caaacatcac cgatactggc accatctgca
     4381 catccagatt tgggttgctg cggtgcaaat caagatagtc gtggaacagc ttaatagact
     4441 cttctttcgg cgtgtaatag gtgaacacac gcggcccgcc gtgaatgaac acatagcgcg
     4501 gcagtagcgt gccgtcgatt tccagcggct ctaacgggtc aggcaagtca tgagccagac
     4561 actgggcgcg caacgtcagc aaatctgctt tcgagttgta cggtaaaacg tacataattg
     4621 gacgagaggt atccagcccc agttccgggg caggatctgc cggaatagac ttgcttttta
     4681 ccaggatgct taatggtaaa ttcagtaatt tgtagtaaat tcgtggccag ccggacataa
     4741 acgatgtaaa gcctctggtt aataatgcaa atgcgcggca aggatagcag aaagtcatgg
     4801 gaaattctgt ggtatccgct catgtttcgc gcggcgttac gcaaacccga atcatcggat
     4861 ttaacggtac actgatattg acgctcataa tgtaaaaagg ttctttcaat ggccaataat
     4921 accactggat tcacccgaat tatcaaagct gctggctatt cctggaaagg tttacgcgct
     4981 gcatggatca acgaagcggc attccgtcag gaaggcgtag cggtattgtt ggcggtggtc
     5041 atcgcctgct ggctggatgt ggacgcaatt acccgcgtgc tgcttatcag ctccgtgatg
     5101 ctggtgatga ttgtggaaat cctcaatagc gccatcgaag cagtggttga ccgaattggc
     5161 tctgaatacc atgagctttc cggacgcgca aaagatatgg gatccgccgc ggtgctgatt
     5221 gccattatcg tcgccgtgat tacctggtgc atcctgttat ggtcgcattt tggataaccc
     5281 ttccagattt cgataaatct ctggtttatt gtgcagttta tggttccaaa atcgcctttt
     5341 gctgtatata ctcacagcat aactgtatat acacccaggg ggcggaatga aagcgttaac
     5401 ggccaggcaa caagaggtgt ttgatctcat ccgtgatcac atcagccaga caggtatgcc
     5461 gccgacgcgt gcggaaatcg cgcagcgttt ggggttccgt tccccaaacg cggctgaaga
     5521 acatctgaag gcgctggcac gcaaaggcgt tattgaaatt gtttccggcg catcacgcgg
     5581 gattcgtctg ttgcaggaag aggaagaagg gttgccgctg gtaggtcgtg tggctgccgg
     5641 tgaaccgctt ctggcgcaac agcatattga aggtcattat caggtcgatc cttccttgtt
     5701 caagccgaat gctgatttcc tgctgcgcgt cagcgggatg tcgatgaaag atatcggcat
     5761 tatggatggt gacttgctgg cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt
     5821 tgtcgcacgt attgatgacg aagttaccgt taagcgcctg aaaaaacagg gcaataaagt
     5881 cgaactgttg ccagaaaata gcgagtttaa accaattgtc gttgaccttc gtcagcagag
     5941 cttcaccatt gaagggctgg cggttggggt tattcgcaac ggcgactggc tgtaacatat
     6001 ctctgagacc gcgatgccgc caggcgtcgc ggtttgtttt tcatctctct tcatcaggct
     6061 tgtctgcatg gcattcctca cttcatctga taaagcactc tggcatctcg ccttacccat
     6121 gattttctcc aatatcaccg ttccgttgct gggactggtc gatacggcgg taattggtca
     6181 tcttgatagc ccggtttatt tgggcggcgt ggcggttggt gcaacggcga ccagctttct
     6241 ctttatgctg ttgctgtttt tacgcatgag caccaccggg ctgactgcgc aggcttatgg
     6301 tgccaaaaat cctcaggcat tagcccgtgc gctggtgcaa ccgttgctgt tggcgttggg
     6361 ggctggggcg ttaattgcgc tgctgcgtac accgattatc gatctggcgc tgcatattgt
     6421 tggcggtagt gaagcggtcc tggaacaggc gcggcgcttt cttgaaatcc gctggttaag
     6481 cgctccggcg tcgctggcga atctggtatt actcggttgg ttactcggcg tgcaatatgc
     6541 ccgtgcgcca gtaattttgt tagtggtcgg caatatcctc aacattgtgc tggatgtctg
     6601 gctggtgatg gggctgcata tgaacgtgca gggcgcggcg ctggcgacgg ttattgcgga
     6661 atatgcaaca ttgctgattg gtctgctaat ggtgcgtaaa atcctcaaac tacgcggaat
     6721 ttccggcgaa atgctgaaaa ctgcctggcg aggaaacttc cgtcgcttgc tggcgcttaa
     6781 ccgcgatatc atgctgcgtt cgctgttgtt gcaactctgt ttcggcgcga tcaccgtact
     6841 tggtgcgcga ctggggagtg acattgtcgc tgttaacgcg gttctgatga cgctactcac
     6901 ctttaccgcc tatgcgcttg atggttttgc ctacgcggtt gaagcgcact ccggtcaggc
     6961 atacggtgcg cgcgacggta gccagttgct ggatgtatgg cgggcagcgt gccgccagtc
     7021 ggggattgta gcgttactgt tttcggtggt ttatttgctg gcaggggaac acatcattgc
     7081 gttgctgacg tcgttaaccc agattcagca gctggctgac cgctatctta tctggcaggt
     7141 gattttgccg ttggttggcg tctggtgtta tctgctggac ggcatgttta taggcgcaac
     7201 gcgtgccgcc gaaatgcgta acagtatggc ggtggccgcc gcaggttttg cgctgacgct
     7261 ccttacgctg ccgtggctgg gtaatcatgg tttgtggctg gcattaaccg tctttctggc
     7321 gttgcgcggg ctttctctgg cggctatctg gcggcgtcac tggcgcaacg gtacctggtt
     7381 tgccgcaacg tgacggttaa aaattctgaa taaataatcc taagccaaat tgctgactac
     7441 acttaatctc acgttcagaa gaaaagtgaa cgtactctca ttcacaacct aacgatgagg
     7501 tcttgattat gaataaagat gaagccggcg gtaactggaa acagtttaaa ggtaaagtga
     7561 aagagcaatg gggcaaactg accgatgatg atatgacgat cattgaaggt aaacgtgatc
     7621 aactggtcgg taaaatccag gaacgttatg gttatcagaa agatcaggca gaaaaagagg
     7681 tcgtggattg ggaaacccgc aatgaatatc gctggtaatt aatccctcct gcccgacgtg
     7741 tacaaggatg tacgccctct taacgcggtt tctttttcac ctgcacagag tgatcatgct
     7801 ggcactgttc aggatgacga cacgcttcca cttccacgca tgccgcacag agcccatgtg
     7861 cttcaatcac attatgccgc agggcaaacc ccatttttgc cgccagcgta tgcataatgt
     7921 cttccacgcc ttctgcacac tcttctttca ccgcgccgca gcgatcgcaa ataaacatgg
     7981 ctgacgtatg ggtgggctgg tcgaacagat gacagagcac ataactgttg gtggattcca
     8041 ccttatgcac aaaaccttgc tcaagcagaa aatccagcgc gcgataaacc gttggcggct
     8101 tggcttgcgg ttcagcttcg cgcagtaaat caagcagatc ataagcgctg atagcgccat
     8161 cctgcagact catcaggcgc aacacttcca ggcgctgtgg ggtcaggcgc acattacgct
     8221 gcgcgcagat tttttcagcc tgcgctaata actcctgcgt tgtggtcttt tccatcgggc
     8281 acctcaaagt gggggttaag aatgtcctta ctttaccatg ttccaggaaa aacaccgaga
     8341 acccttattg ttgccgtaat gttgattttc tgttttgtag gtaaggtgtt gtgttgcctt
     8401 gtcgtaccat tatcaacacg ataataatta ataattcatt tttaaataaa ttgcttcaag
     8461 ttaactcgct atttatttaa ctgggtttaa acgcatacct gtagttcaga aattatgagc
     8521 gatttttgat agtgaccaga tggcaaaaga attataagga ggacacggta cgacaaaaaa
     8581 ggtagatgaa tcatgttgaa gataataccc ggtgcaactg gatactttaa taaaacactc
     8641 aattcaaatc aattcgataa tgaagatgca atcaaagata aattagataa tagaggttca
     8701 atcaaaggta
//
LOCUS       DAZGOB010000168         8705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGOB010000168 DAZGOB010000000
VERSION     DAZGOB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.168"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(15..91)
                     /locus_tag="ACHLIF_004499"
     tRNA            complement(15..91)
                     /locus_tag="ACHLIF_004499"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Asp,seq:gtc)
     gene            complement(224..955)
                     /gene="dnaQ"
                     /locus_tag="ACHLIF_004500"
     CDS             complement(224..955)
                     /gene="dnaQ"
                     /locus_tag="ACHLIF_004500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO9608374.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1020..1487
                     /gene="rnhA"
                     /locus_tag="ACHLIF_004501"
     CDS             1020..1487
                     /gene="rnhA"
                     /locus_tag="ACHLIF_004501"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO9608375.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1484..2206)
                     /locus_tag="ACHLIF_004502"
     CDS             complement(1484..2206)
                     /locus_tag="ACHLIF_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9608376.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2240..2995
                     /gene="gloB"
                     /locus_tag="ACHLIF_004503"
     CDS             2240..2995
                     /gene="gloB"
                     /locus_tag="ACHLIF_004503"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO9608377.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3067..4425
                     /gene="mltD"
                     /locus_tag="ACHLIF_004504"
     CDS             3067..4425
                     /gene="mltD"
                     /locus_tag="ACHLIF_004504"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO9608378.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4473..5243)
                     /locus_tag="ACHLIF_004505"
     CDS             complement(4473..5243)
                     /locus_tag="ACHLIF_004505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9608379.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALID
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5321..6121)
                     /locus_tag="ACHLIF_004506"
     CDS             complement(5321..6121)
                     /locus_tag="ACHLIF_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO9608380.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6362..7276
                     /gene="yafC"
                     /locus_tag="ACHLIF_004507"
     CDS             6362..7276
                     /gene="yafC"
                     /locus_tag="ACHLIF_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO9608381.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7273..8076)
                     /gene="dkgB"
                     /locus_tag="ACHLIF_004508"
     CDS             complement(7273..8076)
                     /gene="dkgB"
                     /locus_tag="ACHLIF_004508"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO9608382.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVIGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8239..8315)
                     /locus_tag="ACHLIF_004509"
     tRNA            complement(8239..8315)
                     /locus_tag="ACHLIF_004509"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8279..8281),aa:Asp,seq:gtc)
     gene            complement(8509..8624)
                     /gene="rrf"
                     /locus_tag="ACHLIF_004510"
     rRNA            complement(8509..8624)
                     /gene="rrf"
                     /locus_tag="ACHLIF_004510"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2289 a         2106 c         2110 g         2200 t
ORIGIN      
        1 ttcatgagtg aatttggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg
       61 caggtattct aaccgactga actaccgctc cgcgttgtgt tccgttggga acgaggcgaa
      121 tattacggat tgcctcaccc ttcgtcaacg gtttttctca tcttttgaat cgtttgctgc
      181 aaaaatcgcc caagccgcta tttttagcgc cttttacagg tatttatgcc cgccagaggc
      241 agcttccgcc cttcttctcc accagatcaa gacgggcttc atgagctgca agctcttcat
      301 ctgtcgcaaa aacaacgcgt aacttacttg cctgacgtac aatgcgctga attgttgctt
      361 caccttgttg ctgctgtgtc tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg
      421 tcatcgccag ataaacttcc gcaaggatct gtgcatcgag taatgccccg tgtagcgttc
      481 gtttactgtt atctatttcg tagcgggcac ataacgcatc gaggctgttg cgcttaccag
      541 gaaacatttt cctcgccacc gcaaggctat cggtgacctt acagaaggta ttggtcttcg
      601 gaatatcgcg cttaagcaac gaaaactcgt agtccataaa gccgatatcg aacgctgcgt
      661 tatggatcac caactccgcg ccgcggatat agtccatgaa ctcatcggct acttcggcaa
      721 acgtgggctt atcgagcaaa aattcatcgg caataccatg tacgccaaag gcttccggat
      781 ccaccagccg atcgggtttg agataaacat ggaagttatt gcccgtcagg cgacggttca
      841 ccacttcaac ggcaccaatc tcaatgatct tgtggccttc atagtgcgca ccaatctggt
      901 tcataccggt ggtttcggta tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg
      961 tcatttatgt cagacttgtc gttttacagt tcgattcaat tacaggaagt ctaccagaga
     1021 tgcttaaaca ggtagaaatt ttcaccgatg gttcgtgtct gggtaatcct ggacctgggg
     1081 gttacggcgc tattttacgc tatcgcggac gcgagaaaac ctttagcgca ggctacaccc
     1141 gcaccaccaa caaccgtatg gagttgatgg ccgctattgt cgcgctggag gcgttaaaag
     1201 aacattgcga agtcattttg agtaccgaca gccagtatgt ccgccaaggt atcacccagt
     1261 ggatccataa ctggaaaaaa cgtggctgga aaacagcaga caaaaaacca gtaaaaaatg
     1321 tcgatctctg gcaacgtctt gatgctgcat tggggcagca tcaaatcaaa tgggaatggg
     1381 ttaaaggcca tgccggacac ccggaaaacg aacgctgtga tgaactggcc cgtgctgcgg
     1441 caatgaatcc cacactggaa gatacaggct atcaagtcga agtttaagcc tgtggtttac
     1501 gatattgccg ggtggctcca accgcctggc gaattcgtgg cttgttttta ctctgtttca
     1561 tcggatttag cgttaaagga atagtccgtt tccgggcaac aataagttgt aagcaaccaa
     1621 gcgcaggaat atgtgcattc aatagttttc caccgtgttt gttccacggg agaacgtgga
     1681 aacggctggc gtgcagcact tcaaaattca acaaagagag ccagtccagt tgccgcatca
     1741 gagtaaacat ccggctgtta tagggcgagg tttttcgcaa taccggcaca agtttgcgta
     1801 atcccattaa actaatggga ttgaagccac taatcaccag ccagccatca tcaatcaata
     1861 cccgatcggt ttcacgcaat aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca
     1921 gtagacaaac atcaacggat ttatcggcaa aaggaagatg aagtggatcc gcctgcacct
     1981 ggacgggcat tccttgcgca gaaacattca cttgatgaga aaccgcgcac gcttcgcaat
     2041 tgatttctgc gcttaaattg ccaatcttaa gcagatgaaa accatacatt ttagtgaacc
     2101 acgggttgag ctggcgctcc agcgccttgc gataaagctc tccccagggc aaatcgcccc
     2161 agcgatcagg agccacgaca gtttgaggga ctcttgccgg tttcatcaca accttccgtt
     2221 tcacactaag aggtaatcta tgaatcttaa cagtattccc gcctttgatg acaattacat
     2281 ctgggttttg aatgatgaag caggtcgctg cctgattgtc gatcccggag acgcagagcc
     2341 agtattaaac gccattgccg ccaataactg gcaaccggag gccatatttc tcacccacca
     2401 tcatcacgat cacgttggcg gcgtaaaaga actggtggaa aagtttccgc aaattgtggt
     2461 gtatggtcca caagagacac aagataaggg aacaacacag gtagtcaaag atggcgaaac
     2521 tgccttcgtt ttggggcatg aatttagtgt aattgccaca ccgggtcaca ctttaggaca
     2581 tatctgttac ttcagtaaac cttatctatt ttgcggcgac acgctgtttt ctggtgggtg
     2641 tggtagattg tttgaaggga cagcatcaca aatgtatcaa tcacttaata agttaagtgc
     2701 gttacctgac gatacattgg tatgttgtgc tcatgaatat acgttatcaa atatgaagtt
     2761 tgctttgagt attcttccgc acgatttgtc cataaatgat tattatcgta aagttaagga
     2821 gttacgggca aaaaatcaaa taacactacc cgtaattctg aaaaatgagc ggcaaattaa
     2881 tgttttttta agaacggaag atattgattt aattaatgta attaatgaag aaacattatt
     2941 gcaacaacct gaagagcgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa
     3001 ctttcttgtc atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac
     3061 acacacatga aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt
     3121 accggcaacg ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca
     3181 aagtttacaa gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac
     3241 ttgtgggctt tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc
     3301 gaacagaaac agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca
     3361 gagccgtata tgtactggat agcagggcaa gttaaaaaac gtaacatgcc tatggaactg
     3421 gtactactac ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc
     3481 gcaggcatct ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc
     3541 aattatgacg cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa tatgatgcag
     3601 cgtctgaaca agatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc
     3661 gaaggtcgag tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc
     3721 tggtcgttac cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt
     3781 gatattctca aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt
     3841 gctctggcgc gtgtgcacct gagcagcccg gttgaaatgg cgaaggttgc agatatggcg
     3901 gggatttccg tcagcaagct gaagacattc aacgctggcg tgaaaggctc cacgctgggc
     3961 gcaagtggtc cgcagtacgt gatggtgcca aagaagcatg cagatcaact gcgtgaatct
     4021 ctggcttcag gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt
     4081 aacagccgtg tttacaccgt acgctctggc gacacgcttt caagtatcgc ttcacgtctc
     4141 ggcgtaagca ccaaagattt gcagcagtgg aacaaactgc gcggatctaa actgaagcca
     4201 ggccaaagtt tgacgattgg cgcaggcagt agcgcacagc gactggcaaa caacagcgat
     4261 agcattacgt atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc
     4321 gtgaacatca aagatgtgat gcgctggaac agcgacactg cgaatctgca accaggcgat
     4381 aagctgacgt tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa
     4441 aaaggcaccg attcccccgg tgcctttttt atttatgccg ctttatgtgc ttctaccatg
     4501 atgatatcac tggtgaaaga gccgtcattc tgcaaggcaa aatacgttct cacctctgtc
     4561 gatgcgctct gttggtaaat gcgaatagcg tctattaacg cttctggcgt acgcattctc
     4621 acgacccatg aagaaaattc cagcggtaac ttatctgtaa ttaaattatc aactatcaga
     4681 ttggcttcat tgattaacgt caaccactca ccgctggcgt agtttcgtac gtgagaggta
     4741 tcgcgtaatg cttctaccgt ctgtaaccag atgtcgcgca ctgggtgacc cggagacatt
     4801 acgtccatca caatcagcct accgccaggt ttcaatatcc ggttcacttc acgcagtgct
     4861 gcaccaacat catgccagtg atgggcagaa taacggctga taacaatatc aaatgcgttg
     4921 tcggcaaatg gcagactttc ggcataaccc tggcgggtgg tgatattttt cagttgccgg
     4981 acttcggcag cttgtgccac gacatctagc atgtgggcag ataagtcata cgccaccacc
     5041 gtgctcacgt tttgcgcagc gacaaagctg gcatgccctg ctccgcagcc catatcaagt
     5101 acacttgcac caggataatc agccagacgc accgccaggc gctgtaaatc tcgtccggat
     5161 gcatgtacgg tactggtcag atattcgctg gcttgagagc taaactgctt ttctacatgg
     5221 tcatggtggg attgtattgt cattgcgctg tccttttgtt atttgagtat aaaagggcag
     5281 cgccaaacgt tggtctgccc tgtggcagac ctgacatacc ttatttatca ggcttgccgg
     5341 gactgaattc aacgagtagc ggattgtgat cggaagcgcg cgtaaccagt acagaagctt
     5401 cgctgacgtt cagaccacgg tagaaaacaa aatcgagcgg gcgaccaaac gcccgacggc
     5461 gctgatcatc ggtaaaacgc acctggcgca gcgacatttc ccgcgcaaag cgatataacg
     5521 cgttcatcct tctacggctc caggcattga aatctcccgc cataatgacc gggccgctgt
     5581 ggtgagctat ctgatcacca ataggaagta actgcttact atagacatcc acgcccagac
     5641 tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg ggtgtcaggt aatggataga
     5701 ccgtcaccag tgccgacttc gccagacgca aaatgggttc tcgttcacgt aacgggcagc
     5761 aatacactgg atgcgccgcc gaaagggtca ttacaccgga aggatgttgt ggcagcacga
     5821 aagcgggcac ctgatcggcg gcaagatagt tagcggtcgc aaactgtact aactctggcg
     5881 ttgtctgcgc ttcctgcaat aacaccagat gtgcatcttt gccgtagttc tttaataccg
     5941 acaaccattc agcgcgttgc tgtttgtata tgttccacac caggatccga atacgctctt
     6001 cagtacttaa cggttctcca ggtggtaatg cctggccgat gctcgcaaaa gaccccggcg
     6061 gtaagatcct ttccgcaggt tgtccggcaa catagcgcat ggcataggtg ttttttcgca
     6121 cttttgactt tgaaacctct attacgtcaa cccttccgca ctcgggaggg ttctactgtt
     6181 atagggattt tagctcactc tttcaacgag caattgtata ttgttatgta agcaagtgct
     6241 tacacaggtc agaattttgc tcattattga gaacattaaa tcggatgaca tatcacgcat
     6301 cctgatggtt taatgacaaa taaattcaaa atatctcttc tattttgcaa caggagcaaa
     6361 aatgaaagcc acgtcggaag aactcgccat ttttgtttcg gtcgtagaaa gcggcagctt
     6421 tagccgggca gcggaacaat tagggcaagc aaactcagcg gtaagccggg cagtgaaaaa
     6481 gctggagatg aaacttggcg ttagcctgct taatcggacc acgcgacaac ttagcctgac
     6541 ggaagaaggc cagcgttatt ttcgtcgcgt acagtcaatt ttgcaggaga tggcagcggc
     6601 agaatcagaa attatggaga cgcgtaatac accgcgtgga ctgttacgca tcgatgccgc
     6661 aactccagtg gtgctgcact ttctgatgcc gttaattaag cctttccgtg aacgctatcc
     6721 ggaagttact ttatcgctag tctcctccga aacgattatt aatttgatcg aaagaaaagt
     6781 ggatgtcgcg atacgcgctg gtacgttaac ggattccagc ttacgtgcca ggccgttatt
     6841 taacagttat cgaaaaatta tcgcctcccc cgattatatt tcccgctacg ggaagccaga
     6901 aacgatcgac gatttaaagc aacatgtttg cctgggattc actgaacccg cttccctcaa
     6961 tacctggccg atagcctgta gtgatggaca attacatgag gtgaagtacg gtttgtcatc
     7021 caatagtggg gaaacactga aacagctttg cctgagtggc aacgggattg cgtgtttgtc
     7081 cgactacatg atcgacaaag aaatcgttcg cggagaattg gtggagttaa tggcagataa
     7141 agtgttgcca gtggaaatgc cattcagtgc agtctattac agcgaccgtg cggtaagtac
     7201 gcgcatccgg gcttttatcg atttccttag cgagcatgta aaaacagctc ccggaggagc
     7261 tgtcagagag gcttaatccc attcaggagc cagaccttcc gggctaacca ggcggtcgtt
     7321 gcaatccagt gcggcgatcg cttttttatc ttcggcatca agctgtaaat tttgtgcctt
     7381 aagattactt tccaggtttt cacgtttagt agaagaagga attactgagt aaccttcccc
     7441 catagcccac gccagaatca cttgtgccgg agtcgcattg tgtttagctg cgatacgagc
     7501 aataacctca tctttcaagg ctttcccata agccagcgtc atataggaag taatatggat
     7561 gccgtgctgt ttagcccagg caaccacttt acggttttgc agataaggag agagttcaat
     7621 ctggttagta gcgatgtttt cagcgccaat aacagcaatc gccttttcca tcaatgggat
     7681 cgtgaagttg gaaataccga tctcacgcgt cagcccttgt tttttggctt ccagcagcgc
     7741 ctgcataaac tcttcaacag agacttcatc gtttggtgac ggccagtgga ttagcgtcag
     7801 atcaacataa tcggtacgca atttttgcag gctctctttc agacttggga tcaatttgtc
     7861 tttgctgaga ttttcaatcc agattttagt ggtgatgtag agttcatgac gtggcacgcc
     7921 actttctgca atcgcctgac ctactgcggc ttcgttatca tagatttgtg cggtatcaat
     7981 tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa ataacaacgt cgtctttcag
     8041 acggaaagta cctaaaccaa atgcagggat agccataata tgcctctttt aatgctattt
     8101 tcgttaactg aggtgattat gggcttcaga ctgttgagga aaaagagcgt aaaatgcaga
     8161 ggatttttgc gattctggca ataatagata tccagacgaa aaaaaacctc gagcgttaac
     8221 tcgaggcttc ttaataagtg gcggaacgga cgggactcga acccgcgacc ccctgcgtga
     8281 caggcaggta ttctaaccga ctgaactacc gctccaccga attcttttac aaccaccggt
     8341 tttatgaccg gcttactgct taatttgatg cctggcagtt tatgaatcac ttcgtgattc
     8401 acccttcggg ccgttgcttc gcaacgttca aaagctttcg cttttgtcct actctcttat
     8461 cgagtagtga actgcctcaa agatcagtgc cattcttcgt aatttgatgc ctggcagttc
     8521 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag
     8581 ttcggcatgg ggtcaggtgg gaccaccgcg ctaaggccgc caggcaaatt ctgttttatc
     8641 agaccgcttc tgcgttctga tttaatctgt atcaggctga aaatcttctc tcatccgcca
     8701 aaaca
//
LOCUS       DAZGOB010000169         8669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGOB010000169 DAZGOB010000000
VERSION     DAZGOB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.169"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(78..956)
                     /gene="glxR"
                     /locus_tag="ACHLIF_004511"
     CDS             complement(78..956)
                     /gene="glxR"
                     /locus_tag="ACHLIF_004511"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO9608383.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1056..1832)
                     /gene="hyi"
                     /locus_tag="ACHLIF_004512"
     CDS             complement(1056..1832)
                     /gene="hyi"
                     /locus_tag="ACHLIF_004512"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO9608384.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(1845..3626)
                     /gene="gcl"
                     /locus_tag="ACHLIF_004513"
     CDS             complement(1845..3626)
                     /gene="gcl"
                     /locus_tag="ACHLIF_004513"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO9608385.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3716..4531)
                     /gene="allR"
                     /locus_tag="ACHLIF_004514"
     CDS             complement(3716..4531)
                     /gene="allR"
                     /locus_tag="ACHLIF_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO9608386.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4609..5091)
                     /gene="allA"
                     /locus_tag="ACHLIF_004515"
     CDS             complement(4609..5091)
                     /gene="allA"
                     /locus_tag="ACHLIF_004515"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO9608387.1"
                     /translation="MKLQVLLLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5321..6247
                     /gene="allS"
                     /locus_tag="ACHLIF_004516"
     CDS             5321..6247
                     /gene="allS"
                     /locus_tag="ACHLIF_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO9608388.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6316..7410
                     /gene="mnmH"
                     /locus_tag="ACHLIF_004517"
     CDS             6316..7410
                     /gene="mnmH"
                     /locus_tag="ACHLIF_004517"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO9608389.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            7526..7891
                     /gene="ylbG"
                     /locus_tag="ACHLIF_004518"
                     /pseudo
     CDS             7526..7891
                     /gene="ylbG"
                     /locus_tag="ACHLIF_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(8412..>8669)
                     /locus_tag="ACHLIF_004519"
     CDS             complement(8412..>8669)
                     /locus_tag="ACHLIF_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608390.1"
                     /translation="DYFFSYFSLTNDDGGRFKKTLTVRAREQVAKIVSKNDPDTKKVW
                     CKYGKIPGQGDGVNLFFVGEINVTHYFITNIGAGLPDACAE"
BASE COUNT         2220 a         2269 c         2033 g         2147 t
ORIGIN      
        1 caacatcttt ggctgctttc ttttttaata ttgctggccg attcattttc tgatatcggc
       61 catttttatt gcgggtatca ggccagttta tggttagcca ttaattccag cgcctgcact
      121 aacgcagagt gatccaactg gctgccaccg tttgccgcgc aggtattaaa taactcctgg
      181 caggtcgcag tgtttggcag gttcagcgct agcgctttcg cactttgcag tgccaggttg
      241 agatctttct ggtgcagcgc aattttgaaa cctggattaa aagtgcgttt aatcatgcgc
      301 tcaccgtgaa cttccagaat acgtgaggaa gcaaagccgc ccatcagcgc ctggcgcaca
      361 cgtaccgggt ccgcaccggc ttttgaagca aatagcaggg cttcagaaac cgcttcaata
      421 ttgagcgcca caataatctg atttgccact ttgcaggttt gaccatcgcc gttaccgccc
      481 acgagggtga tatttttacc gagcagttca aacagcggtt taacacgttc aaataccgct
      541 tcatcaccgc caaccataat cgacaacgtc ccttcacgcg caccgatttc accgccggag
      601 actggcgcat cgagataatc gccgcccagt tcattcacct gacgagcgaa acgcttagtt
      661 tcaatcgggg aaatggagct catatcaaca atggttttgc ccttcagcga ggctttggta
      721 caaccatttt caccgaacag aacttcttca acctgtggtg tatctggcac cataataaaa
      781 atgatgtccg atgcttccgt tacctggcgc gcagtttcaa cactgacggc acccagtgac
      841 agtaattcat cagcaaccgg accaatggtt gtgacatgca gttgatgacc ggcacgcgcc
      901 agattaatgg ccatcggtgt acccataatg cctaagccaa taaatcccag tttcataatt
      961 aacctctttt aaatttcgct tttcctgaat tcagacaaca cgatcccctg ccaacttagc
     1021 ggcctaaaaa agcattgcct gaacagcgtt acgttttaac ggtacggatc catccagcgt
     1081 aaaccggctt ccgtggtggt ttggggtgta tattcacacc caacccaacc gttgtagtca
     1141 gaattttcga ttaccttaaa gagataatca taattaattt ctccggtccc cggttcgccg
     1201 cgatgcggat tatcagcaat ttgcaggtga ccaattttat cggcccactg agtcatggtg
     1261 ttggttaatt caccttccat ccgctgcatg tgataaatgt catactgaat tttcaaattg
     1321 cagcaaccaa catcatcaat caatttcagc gcctgccgag ttccggtgag atggaaacca
     1381 ggaatatcaa aatggttaat aggttcaatc agtaataaaa tatcttcttt catcagcata
     1441 ttcgcggcat aacgcaggtt ttctacaagc gttgcgtgaa tctgttcact gctgaaacca
     1501 gccggcgttt taccgaccag acagttaatt tttttattac ccagcgcacg ggcataacga
     1561 atcgctgctg ctacgccatc ccgaaactct tcttcacggc caggaataca ggcaataccg
     1621 cgctcccccg ccgcccagtc acctgccggt aaattgtgca gcgtatgttc gagtttatta
     1681 ctcgccagca cctgttttaa ttcttcaatg tcgtagtcat aaggaaacat aaattcaacg
     1741 ccgcgaaaac cacactgcgc agctttctca aaacgggcga gaaaatcata ttcaccaaat
     1801 aacatcgata aattagcaga gaaacgtaac attatttatc tcccttattc atagtgcatg
     1861 aagcaggttt cagtcggtgc gtccgctgcg ttatcggcga tatcttcaaa ttccatgacg
     1921 ttatccagtt cgctgcccat cgaaatattg gtcacacgct caagaataac ttccacgact
     1981 accggtaccc gatattgcgc cattaaggct ttcgcctgtt caaaggctgg cgcaatatct
     2041 tccggtttga agacccgaat agctttacaa cctaaacctt ccgctacttt tacgtggtcg
     2101 acgccgtaac cgttcacttc actggagtta atattttcga acgcgagctg tacgcagtaa
     2161 tccatgtcaa aggcgcgttg tgactgacga atcagcccca gataagcgtt attgaccagt
     2221 acatggatgt acggaatgtt gaactgtgcg ccaacggcta attcttcaat caggaactgg
     2281 aagtcgaagt caccggaaat cgccaccaca ttccgttctg gatcagcagc acacacgccc
     2341 agcgccgccg gaatggtcca gcccagcggg cctgcctgac cacagttgat ccagtgacgg
     2401 tctttaaaga catgcagcat ttgtgccgca gcgatttgtg acaggccaat ggtggtgaca
     2461 taacaaacat cgcgaccaaa ggctttgttc atctcttcat acacgcgctg cggtttcacc
     2521 ggcacgttgt cgaagtgggt tttgcgcagc aaagtgcgtt tgcgctgctg gcagtcggcg
     2581 acccattctt tacgacacgg cagacgaccc gctttttgca tctcctgcgc cacttcaacc
     2641 agcagtgtca gcgccgcttt agcatcagag acaatgccga gatccggaca cagcacgcga
     2701 ccaatttgcg tcggctcaat atcgatatga acgattttgc gcccttcggt gtatttctct
     2761 accgaaccgg tatgacggtt agcaaaacgg ttaccgatac caaacaccat gtcggacgcc
     2821 agcagcgttg cgttaccgta acgatgcgcg gtttgcagac ccaccatccc ggccatcagt
     2881 tcatgatcgt ccgggataca gccccagccc atcagcgttg ggatcaccgg aacgctggtc
     2941 agttcagcaa actgttgtaa cagtgcagct gcgtcagcat taattacccc gcccccggca
     3001 acaatcaccg gacgttcggc ctggattaac atttctacag ctttttcgat ctgcatacgg
     3061 ctggcagcag gtttgtagac cggcagcggt tcgtacatat caggatcaaa ctcgatttcc
     3121 gcaacctgaa cgtcgaacgg taaatccacc agtaccggac ccggacgacc agaacgcatc
     3181 aggtgaaatg cctgttgcaa cacacgaggc accagcgccg cttcacgtac tgtaaccgcc
     3241 attttactta ccggcttggc gatagcttcg atatcaacag cctggaaatc ttctttatgc
     3301 agacgggcgc gcggagcctg gccagtaata cacagaatag gaatggaatc agcagaagca
     3361 gaatagagcg cggtgatcat gtccgtgccc gcaggaccgg aagtccccag acatacgccg
     3421 atattccctg ccgttgcgcg ggtataacct tccgccatgt gcgaagcacc ttccacatga
     3481 cgcgccagaa tgtgacgaat accgccgtgc ttacgcatcg ctgagtagaa cggattgatt
     3541 gcagctcccg gaacgccgaa ggcggtggtg ataccttctt tctccagcac atacattgcc
     3601 gcgtcaacgg ctctcatttt tgccatttta ttccaacctc tattaattgg aaaaattttc
     3661 caactttcaa attttgtcaa aaaaacccgc tccgcagagc gggatgcgac agacactatg
     3721 gatgtgcttt cagtcccaac gccgtgctga tatcgcgggc ggtgtctctg accagctcac
     3781 cctgactgac aaaacgatct tctgtcagtc ttgatgaagg cccggagatg gagatagcgg
     3841 caacaacgct accgacatca tcgtaaattg ctgaagctat gcaattcaga cctacaacat
     3901 gctcttcttt atctacggta tagcccagtt cacgcgcttg ttccaggtcc ttcagcaagg
     3961 tgggcatatc cacaagcgta gttggcgtaa actgctgcaa accggtttgc agaatgatgc
     4021 tcatcaactc ctcttccgcc agcggataaa gcaacgcctt gcccgcaccg gaagcatgca
     4081 gcggcagacg actgcccagt ggcgcacaca tcctgaccat cgatttacac tctaactgac
     4141 caattaatac cgcttcattg ccgttacgga tcgcgacatt gaccgtttcg ccggaaagta
     4201 acatcaggcg gcgcataaac ggcccggcga cggagaggac atcgcggtta tggatgtacg
     4261 ccgcaccgac gttaaagaca cctaatccta tatgccacca gcctaactga ctgtcctgat
     4321 agacaaaatc cgctgcctgt aaaaccttca gcaagcgaaa ggtcgtagag agcggcaaat
     4381 ccagattgag agaaatatcg ctaaccgacg aacttccccc acttttttcc agatattgca
     4441 gaatcgcaat tccccgctct aacgcctgtg cgcccttctg tgccacaggc tccgcctgtc
     4501 ctggcctgcc gcgccgtcta acttccgtca tacctacgtt aactccgacc tacaagtgag
     4561 cataaacaat gaacaattag tttatcttat gcaagtcggt tgcaggcgtc acgcaaaaca
     4621 gagttcctgt tcaggaatac tttcaacatc acagttgtca ctgccgccgc gatcgatggt
     4681 cagaaaatcg gtgacgcgct gccaggcgaa aagtgggtga tgccagacgt tacggtggta
     4741 attcactccc tgttcgccgt tggtaataaa cgcccgcagg gttgacaggt ctggtttgtc
     4801 gtcacctaac gccacgacca ccacaaacac ctcacctttc atcgggataa aggcctgagt
     4861 acccagcgga tgacgttcga gttcgtgaat ggtcagcggc agattcgccg gttgcgcgcg
     4921 gttaatgctg ataagcgtac ggtcttgctc aagaatctca accagcgcca aatcgtggta
     4981 acgctccacc aggccattgt taatatggaa aaaatcccgt tgctgcgttt cgattacgtc
     5041 gccataagca ctaaaggctt cctgacttaa cagtaatacc tgaagtttca tctttttgtc
     5101 cttgtaacat aattccgttc aaccaccgcc gaccggtcag cttggtttca ataacaggca
     5161 atctaaaacc ttaagagaac aacatcaata caaaagttgg aatatttttc caaaactaga
     5221 tttagtccac aaattgaaca gggttatcat gtaaaaatac tgataaatca gaggataaga
     5281 aaatcacgca acttgcggat ttaaaagggg atacctgaag atgttcgatc cagaaacctt
     5341 gcggactttc attgcggttg ctgaaacagg aagtttttca aaagcggcag aacgattatg
     5401 taaaaccacg gcgacgatca gttatcgcat taaacttctg gaagagaata ccggagtagc
     5461 actgtttttc cgcacgactc gcagcgtgac gttgacagcg gctggcgagc atctactttc
     5521 ccaggccaga gactggctga gctggctgga aagtatgcca agcgagctgc aacaggtgaa
     5581 tgatggcgtg gaacgccagg tgaatattgt catcaacaac ctgctctaca acccccaggc
     5641 cgtcgcccag ttgctggcgt ggctgaatga acgttacccc tttacccagt ttcacatctc
     5701 ccgacaaatc tatatgggcg tctgggattc gctattatac gaaggttttt cactggctat
     5761 cggcgtcacg ggaaccgagg cgctggcaaa tacctttagt cttgatccct taggatcggt
     5821 gcaatggcgc tttgtcatgg cggcggatca tccgctggcg aacgttgaag agccgctaac
     5881 agaagcgcag ttgcggcgct ttccggcggt caatattgaa gacagcgccc gcaccttaac
     5941 caaacgcgtc gcctggcgat tgcctgggca aaaagagatt attgttcctg atatggaaac
     6001 gaaaatcgcc gcccatcttg cgggcgttgg cattggtttt ttgccaaaat cgctttgcca
     6061 gtcaatgatc gataatcaac aactagttag ccgggtaatc ccaacgatgc gccctccttc
     6121 gccattgagt ctggcatggc gcaaatttgg cagcggcaaa gcggtagaag atattgtgac
     6181 cttgtttacc cagcgcaggc cggaaatcag cggattttta gaaattttcg gcaacccacg
     6241 cagttaaaaa tcaagtttcc ttgcgcgttg gcgcgtaaaa tagcgcgcaa gctgatttga
     6301 ggttcacttt taactatgca agagagacac acggaacagg actatcgtgc cctgctgatt
     6361 gctgatacgc ccattattga tgttcgcgcc cctatcgagt ttgagcaagg cgcaatgccc
     6421 gccgctatca atctgccgtt aatgaataac gatgaacgcg ccgccgttgg cacctgctat
     6481 aaacagcaag gctcagacgc agcgctggcg ctggggcata aactggtggc gggtgaaatt
     6541 cgtcagcagc gcatggacgc ctggcgggca gcgtgcctgc aaaatccgca aggtattctc
     6601 tgctgcgccc gtggcggtca gcgctcacat attgtgcaaa gctggttgta tgcagcgggg
     6661 attgattatc cgctggtgga aggcggttat aaggcactgc gccagaccgc gattcaggcg
     6721 actattgaac tggcacaaaa accgatagtg ctgattggcg gttgtactgg cagcggtaaa
     6781 acgctgttag tgcagcaaca gccgaacggt gttgatctgg aagggctggc gcgccatcgc
     6841 ggttcggcgt ttggtcgcac gttacaacca caacttagcc aggcgagttt tgaaaacctg
     6901 ctggctgccg aaatgctgaa aaccgacgcc cgtcaggatt tgcgcctgtg ggtgctggaa
     6961 gacgaaagcc ggatgatcgg ttcgaatcac ctgccggaat gcctgcgcga gcgaatgact
     7021 caggcggcga ttgcggtggt agaagatccg tttgagatcc gtcttgagcg cctgaacgaa
     7081 gagtatttct tgcgtatgca tcatgatttt acccacgctt atggcgacga acagggctgg
     7141 caggaatatt gcgaatacct gcatcacgga ctttcggcga ttaagcgtcg gctggggtta
     7201 cagcgctata acgaactcgc tgcccagctg gatacggcgc tgacaacgca actcaccacc
     7261 ggcagcaccg acggtcatct ggcctggctg gtgccgttac ttaaagaata ttacgaccca
     7321 atgtatcgct atcagcttga gaaaaaagcg gaaaaagttg tctttcgcgg tgagtgggcg
     7381 gaagtggcgg aatgggttaa ggcgcggtaa tgagggacgt atttgtatta gtcgcgactt
     7441 gatctgacac atggccttga aaggttgaga gttaccggtt ttgatatgag tgtcgaatcc
     7501 ttatccaaaa caagaggtaa ctctcatgct tcatactgcc aatcccgtca tcaaacacaa
     7561 agccggtttg ctcaatctgg ctgaagaact cagcaacgta tcaaaagcct gtaaaatcat
     7621 gggcgtttcg cgtgatacgt tttatcgtta ccgcgaactg gtcgctgaag gcggtgtgga
     7681 tgcgcagatt aatcgtagtc gccgcgctcc taaccttaag aaccgtaccg atgaggcaac
     7741 tgaacaggct gttgttgatt acgccgtcgc tttcccgaca catggtcagc accgggccag
     7801 caacgagctg cgtaaacagg gggtttttat ctccgacagt ggtgtccgtt ctgtctggtt
     7861 actccataac cttgagaatc tcaaaagacg atactgaaca aattttatca ggtgacgttc
     7921 cgtaaaaagt tgtatggagt tctcgatgca gtcggcgcga ctatcagtac ccccgctgaa
     7981 ccctggacta tcgggccgcc gtactggggc atgtcaccct ggcgtgccgc tggttttccg
     8041 ctaatggttt atttcctctt tctttcccgg caagaattta ttttaatccg gaaggattat
     8101 ttattttcag atagggactc acattaaaca cgtaacattt taattaatgc atggctgaat
     8161 atgtattaca caatgtcaga tatcgtaaaa gaccagtgca attctatgta aactctctga
     8221 ctgttcattt tatttgttgt ttcagggtcg gaactcaccc tcaataaaca actgaatacg
     8281 agtagatatt catcgtctga gctatatggc tttacacaat agccgacatg tcttttctca
     8341 gtcaggaatt ttacttttcc tcactacgaa gttgacgact tcgatatggg atagactctt
     8401 aattcaagca attactctgc acaagcatca ggcaatccag ctccaatatt tgttataaaa
     8461 taatgcgtaa cattaatttc accaacaaaa aaaaggttta caccatcccc ttgccctggt
     8521 atcttaccat atttacacca cacttttttt gtatctggat cattctttga tacgattttc
     8581 gccacttgtt ccctggccct cactgtcaat gttttcttaa acctaccccc atcatcattt
     8641 gttagggaaa aataagaaaa aaaataatc
//
LOCUS       DAZGOB010000170         8655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGOB010000170 DAZGOB010000000
VERSION     DAZGOB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.170"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(285..548)
                     /gene="rpsT"
                     /locus_tag="ACHLIF_004520"
     CDS             complement(285..548)
                     /gene="rpsT"
                     /locus_tag="ACHLIF_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO9608391.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            651..869
                     /locus_tag="ACHLIF_004521"
     CDS             651..869
                     /locus_tag="ACHLIF_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO9608392.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            877..1818
                     /gene="ribF"
                     /locus_tag="ACHLIF_004522"
     CDS             877..1818
                     /gene="ribF"
                     /locus_tag="ACHLIF_004522"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO9608393.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLGVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1861..4677
                     /gene="ileS"
                     /locus_tag="ACHLIF_004523"
     CDS             1861..4677
                     /gene="ileS"
                     /locus_tag="ACHLIF_004523"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO9608394.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4677..5171
                     /gene="lspA"
                     /locus_tag="ACHLIF_004524"
     CDS             4677..5171
                     /gene="lspA"
                     /locus_tag="ACHLIF_004524"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO9608395.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5259..5708
                     /gene="fkpB"
                     /locus_tag="ACHLIF_004525"
     CDS             5259..5708
                     /gene="fkpB"
                     /locus_tag="ACHLIF_004525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9608396.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5710..6660
                     /gene="ispH"
                     /locus_tag="ACHLIF_004526"
                     /gene_synonym="lytB"
     CDS             5710..6660
                     /gene="ispH"
                     /locus_tag="ACHLIF_004526"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO9608397.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6726..7640
                     /gene="rihC"
                     /locus_tag="ACHLIF_004527"
     CDS             6726..7640
                     /gene="rihC"
                     /locus_tag="ACHLIF_004527"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO9608398.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7807..8628
                     /gene="dapB"
                     /locus_tag="ACHLIF_004528"
     CDS             7807..8628
                     /gene="dapB"
                     /locus_tag="ACHLIF_004528"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO9608399.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1963 a         2156 c         2470 g         2066 t
ORIGIN      
        1 agagtggtct taagtttaat acctcaatgt gtatcacaat atccacattc tttgtagggg
       61 agtctggaga ttgagtagat attcttgttc agaatgtatc agccgatggt tctacgattc
      121 ttaagccacg aagagttcag aaagtgtaac ggcatgtctg caaaagccag catcttttga
      181 ctatctggca accggtagtg tgttctctca cgcatcataa aagcagcagg cataaaaaaa
      241 cccgcttgcg cgggcttttt cacaaagctt cagcaaattg gcgattaagc cagtttgttg
      301 atctgtgcag tcaggttagc cttatgacgt gcagctttgt ttttgtggat cagaccttta
      361 gcagcctgac ggtccacgat cggttgcatt tcgttaaatg ctttctgtgc agcagctttg
      421 tcgccagctt cgatagctgc gtatactttc ttgatgaaag tacgcatcat agagcgacgg
      481 cttgcgttgt gcttacgagc cttttcagac tgaatggcgc gcttcttagc tgatttgata
      541 ttagccaagg tccaactccc aaatgtgttc tatatggaca attcaaaggc cgaggaatat
      601 gcccttttag ccttcttttg tcaatggatt tgtgcaaata agcgccgtta atgtgccggc
      661 actcgttacg tagtgatggc gcaggattct accagcttgc agggtgtgaa tacagctttt
      721 ccgcgataaa aattgcagca ggcggtcact ttcttcccgt gatttgcgcc atggcaatga
      781 aaagccactt ctttctgatt tcggtactca atcgccggtt aaccttgacc gctgtacaag
      841 gtatactcgg acgattttca ctgttttgag ccagacatga agctgatacg cggcatacat
      901 aatctcagcc aggccccgca agaagggtgt gtgctgacta ttggtaattt cgacggcgtg
      961 catcgcggtc atcgcgcgct gttacagggc ttgcaggaag aagggcgcaa gcgcaactta
     1021 ccggtgatgg tgatgctttt tgaacctcaa ccactggaac tgtttgctac cgataaagcc
     1081 ccggcacggc tgacccggct gcgggaaaaa ctgcgttacc ttgcagagtg tggcgttgat
     1141 tacgtgctgt gcgtgcgttt cgacaggcgt ttcgcggcgt taaccgcgca aaatttcatc
     1201 agcgatcttc tggtgaagca tttgcgcgta aaatttcttg ccgtaggtga tgatttccgc
     1261 tttggcgctg gtcgtgaagg cgatttcttg ttattacaga aagctggcat ggaatacggc
     1321 ttcgatatca ccagtacgca aactttttgc gaaggtggcg tgcgtatcag cagcaccgct
     1381 gtgcgtcagg cgctggcgga tgacaatctg gctctggcag aaagtttact ggggcacccg
     1441 tttgccatct ccgggcgtgt agtccacggt gatgaattag ggcgcactat aggtttcccg
     1501 acggcgaatg taccgctacg ccgtcaggtt tccccggtga aaggggttta tgcggtagaa
     1561 gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa acattggaac acgcccaacg
     1621 gttgccggta ttcgccagca gctggaagtg catttgttag gtgttgcaat ggacctttac
     1681 ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca atgagcagcg atttgcgtcg
     1741 ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa ccgcccgcga attttttggg
     1801 ctaacaaaac cggcttaagc ctgttatgta atcaaaccga aacacggaac cgagaatctg
     1861 atgagtgact ataaatcaac cctgaatttg ccggaaacag ggttcccgat gcgtggcgat
     1921 ctcgccaagc gcgaaccggg aatgctggcg cgttggactg atgatgatct gtacggcatc
     1981 atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc atgatggccc tccttatgcg
     2041 aatggcagca ttcatattgg tcactcggtt aacaagattc tgaaagacat tatcgtgaag
     2101 tccaaagggc tttccggtta tgactcgccg tatgtgcctg gctgggactg ccacggtctg
     2161 ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg gtgagaaatt caccgccgcc
     2221 gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg ttgacggtca acgcaaagac
     2281 tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt acctgaccat ggacttcaaa
     2341 actgaagcca acatcatccg cgcgctgggc aaaatcatcg gcaacggtca cctgcacaaa
     2401 ggcgcgaagc cagttcactg gtgcgttgac tgccgttctg cgctggcgga agcggaagtt
     2461 gagtattacg acaaaacttc tccgtccatc gacgttgctt ttcaggcggt cgatcaggat
     2521 gcactgaaag caaaatttgc cgtaagcaac gttaacggcc caatctcgct ggtgatctgg
     2581 accactacgc cgtggactct gcctgcgaac cgcgcaatct ctattgcacc tgatttcgac
     2641 tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg cgaaagatct ggttgaaagc
     2701 gtaatgcagc gtatcggcgt gaccgattac accattctcg gcacggtaaa aggtgcggag
     2761 cttgagttgc tgcgctttac ccatccgttt atgggcttcg acgttccggc aatcctcggc
     2821 gatcacgtta ccctggatgc cggtaccggt gccgttcaca ccgcgcctgg ccacggcccg
     2881 gacgactatg tgatcggtca gaaatacggc ctggaaaccg ctaacccggt tggcccggac
     2941 ggcacttatc tgccgggcac ttatccgacg ctggatggcg tgaacgtctt caaagcgaac
     3001 gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc tgcacgttga gaaaatgcag
     3061 cacagctatc cgtgctgctg gcgtcacaaa acgccgatca tcttccgcgc gacgccgcag
     3121 tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt cactgaaaga gatcaaaggc
     3181 gtgcagtgga tcccggactg gggccaggcg cgtatcgagt cgatggttgc taaccgtcct
     3241 gactggtgta tctcccgtca gcgcacctgg ggcgtaccga tgtcactgtt cgtgcacaaa
     3301 gacacggaag agctgcatcc gcgtactctc gaactaatgg aagaagtggc aaaacgcgtt
     3361 gaagttgacg gcatccaggc gtggtgggat cttgatgcga aagagatcct cggcgatgaa
     3421 gctgatcagt acgtgaaagt gccggacaca ttggatgtat ggtttgactc cggatctacc
     3481 cactcttctg ttgttgacgt gcgtccggaa tttgccggtc acgcagcgga catgtatctg
     3541 gaaggttctg accagcaccg tggttggttc atgtcttccc taatgatctc caccgcgatg
     3601 aaaggcaaag cgccgtatcg tcaggtactg acccacggct ttaccgtgga tggtcagggt
     3661 cgcaagatgt ctaaatccat cggcaatacc gtttcgccgc aggatgtgat gaacaaactg
     3721 ggcgcggata ttctgcgtct gtgggtggca tcaaccgact acaccggtga aatggccgtt
     3781 tctgacgaga tcctgaaacg tgctgccgat agctatcgtc gtatccgtaa caccgcgcgc
     3841 ttcctgctgg caaacctgaa cggttttgat ccagcaaaag atatggtgaa accggaagag
     3901 atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag cggcacagga agacatcctc
     3961 aaggcgtacg aagcatacga tttccacgaa gtggtacagc gtctgatgcg cttctgctcc
     4021 gttgagatgg gttccttcta cctcgacatc atcaaagacc gtcagtacac cgccaaagcg
     4081 gacagcgtgg cgcgtcgtag ctgccagact gcgctgtatc acatcgctga agcgctggtg
     4141 cgctggatgg caccaatcct ctccttcacc gctgatgaag tgtggggcta tctgccgggc
     4201 gaacgtgaaa aatacgtctt taccggtgag tggtacgaag gcctgtttgg tctggcagac
     4261 agtgaagcga tgaacgatgc gttctgggac gagctgttga aagtgcgtgg cgaagtgaac
     4321 aaagtcattg agcaagcgcg tgccgataag aaagtgggtg gctcgctgga agcggcagta
     4381 accttgtatg cagaaccgga actggcggcg aaactgaccg cgctgggcga tgaattacga
     4441 tttgtcctgt tgacctccgg cgctaccgtt gcagactata atgacgcacc tgctgatgct
     4501 cagcagagcg aagtactcaa agggctgaaa gtcgcgttga gtaaagccga aggtgagaag
     4561 tgcccacgct gctggcacta cacccaggat gtcggcaagg tggcggaaca cgcagaaatc
     4621 tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa aacgtaagtt tgcctgatga
     4681 gtcaatcgat ctgttcaaca gggctacgct ggctgtggct ggtggtagtc gtgctgatta
     4741 tcgatctggg cagcaaatac ctgatcctcc agaactttgc tctgggggat acggtcccgc
     4801 tgttcccgtc gcttaatctg cattatgcgc gtaactatgg cgcggcgttt agtttccttg
     4861 ccgatagcgg cggctggcag cgttggttct ttgccggtat tgcgattggt attagcgtga
     4921 tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa gctaaacaat atcgcttatg
     4981 cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg cctgtggcac ggcttcgttg
     5041 tcgatatgat cgacttctac gtcggcgact ggcacttcgc caccttcaac cttgccgata
     5101 ctgccatttg tgtcggtgcg gcactgattg tgctggaagg ttttttgcct tctaaagcga
     5161 aaaaacaata ataaaccctg ccggataagg cctacagatt gctgcgaaat cgtaggccgc
     5221 atccggcaaa aatcctcaaa atataagagc aaacctgcat gtctgaatct gtacagagca
     5281 atagcgccgt cctggtgcac ttcacgctga aactcgatga tggcaccacc gctgagtcta
     5341 cccgcaacaa cggtaaaccg gcgctgttcc gcctgggcga tgcttctctt tctgaagggc
     5401 tggagcaaca cctgttgggg ctgaaagtgg gcgataaaac caccttctcg ctggagcccg
     5461 atgcggcgtt tggcgtgccg tcaccggacc tgattcagta cttctcccgc cgtgaattta
     5521 tggatgcagg cgagccagaa attggcgcaa ttatgctttt taccgcaatg gatggcagtg
     5581 agatgcctgg cgtgatccgc gaaattaacg gcgactccat taccgttgat ttcaaccatc
     5641 cgctggccgg gcagaccgtt cattttgata ttgaagtgct ggaaatcgat ccggcactgg
     5701 aggcgtaaca tgcagatcct gttggccaac ccgcgtggtt tttgtgccgg ggtagaccgc
     5761 gctatcagca ttgttgaaaa cgcgctggcc atttacggcg caccgatata tgtccgtcac
     5821 gaagtggtac ataaccgcta tgtggtcgat agcttgcgtg agcgtggggc tatctttatt
     5881 gagcagatta gcgaagtacc ggacggcgcg atcctgattt tctccgcaca cggtgtttct
     5941 caggcggtac gtaacgaagc aaaaagtcgc gatttgacgg tatttgacgc gacctgtccg
     6001 ctggtgacca aagtgcatat ggaagtcgcc cgcgccagtc gccgtggcga agaatctatt
     6061 ctcatcggtc acgccgggca cccggaagtg gaagggacaa tgggccagta cagtaacccg
     6121 gaagggggaa tgtatctggt cgaatcgccg gacgatgtgt ggaaactgac ggtcaaaaac
     6181 gaagagaagc tctcctttat gacccagacc acgctgtcgg tggatgacac gtctgatgtg
     6241 atcgacgcgc tgcgtaaacg cttcccgaaa attgtcggtc cgcgcaaaga tgacatctgc
     6301 tacgccacga ctaaccgtca ggaagcggta cgcgccctgg cagaacaggc ggaagttgtg
     6361 ttggtggtcg gttcgaaaaa ctcctccaac tccaaccgtc tggcggagct ggcccagcgt
     6421 atgggcaaac gcgcgttttt gattgacgat gcgaaagata tccaggaaga gtgggtgaaa
     6481 gaggttaaat gcgtcggcgt gactgcgggc gcatcggctc cggatattct ggtgcagaat
     6541 gtggtggcac gtttgcagca gctgggcggt ggtgaagcca ttccgctgga aggccgtgaa
     6601 gaaaacattg ttttcgaagt gccgaaagag ctgcgtgtcg atattcgtga agtcgattaa
     6661 gtcattagca gcctaagtta tgcgaaaatg ccggtcttgt taccggcatt ttttatggag
     6721 aaaacatgcg tttacctatc ttcctcgata ctgaccccgg cattgacgat gccgtcgcca
     6781 ttgccgccgc gatttttgca cccgaactcg acctgcaact gatgaccacc gtcgcgggta
     6841 atgtctcggt tgagaaaact acccgcaatg ccctgcaact gctgcatttc tggaatgcgg
     6901 agattccgct cgcccaaggg gccgctgtgc cgctggtacg cgcaccgcgt gatgcggcat
     6961 ctgtgcacgg cgaatcggga atggctggct acgactttgt tgagcacaac cgaaagccgc
     7021 tcgggatacc ggcgtttctg gcgattcggg atgccctgat gcgtgcacca gagcctgtta
     7081 ccctggtggc catcggcccg ttaaccaata ttgcgctgtt actttcacaa tgcccggaat
     7141 gcaagccgta tattcgccgt ctggtgatca tgggtggttc tgccggacgc ggcaactgta
     7201 cgccaaacgc cgagtttaat attgctgccg atccagaagc tgctgcctgt gtcttccgca
     7261 gtggtattga aatcgtcatg tgcggtttgg atgtcaccaa tcaggcaata ttaactcctg
     7321 actatctctc tacactgccg cagttaaacc gtaccgggaa aatgcttcac gccctgttta
     7381 gccactaccg tagcggcagt atgcaaagcg gcttgcgaat gcacgatctc tgcgccatcg
     7441 cctggctggt gcgcccggac ctgttcactc tcaaaccctg ttttgtggca gtggaaactc
     7501 agggcgaatt tacctcaggc acgacggtgg ttgatatcga cggttgcctg ggcaagccag
     7561 ccaatgtaca ggtggcattg gatctggatg tgaaaggctt ccagcagtgg gtggctgagg
     7621 tgctggctct ggcgtcgtaa cctgtcacat gttattggca tgcagtcatt catcgactca
     7681 tgcctttcac tgatatccct ccctgtttat cattaatttc taattatcag cgtttttggc
     7741 tggcggcgta gcgatgcgct ggttactctg aaaacggtct atgcaaatta acaaaagaga
     7801 atagctatgc atgatgcaaa catccgcgtt gccatcgcgg gagccggggg gcgtatgggc
     7861 cgccagttga ttcaggcggc gctggcatta gaaggcgtgc agctgggcgc ggcgctggag
     7921 cgcgaaggat cttctttact gggcagcgac gccggtgagc tggctggtgc agggaaaaca
     7981 ggcgttaccg tgcaaagcag cctcgatgcg gtaaaagatg attttgatgt gtttatcgat
     8041 tttacccgtc cggaaggtac gctgaaccat ctcgcttttt gtcgccagca tggcaaaggg
     8101 atggtgatcg gcactacggg gtttgacgaa gccggtaaac aggcgattcg tgacgccgcc
     8161 gccgatattg cgattgtctt tgccgccaat tttagcgttg gcgttaacgt catgcttaag
     8221 ctgctggaga aagcagccaa agtgatgggt gactacaccg atatcgaaat tattgaagca
     8281 catcatagac ataaagttga tgcgccgtca ggcaccgcac tggcaatggg agaggcgatc
     8341 gcccacgccc ttgataaaga tctgaaagat tgcgcggtct acagtcgtga aggccacacc
     8401 ggtgaacgtg tgcctggcac cattggtttt gccaccgtgc gtgcaggtga catcgttggc
     8461 gaacataccg cgatgtttgc cgatattggc gagcgtctgg agatcaccca taaggcgtcc
     8521 agccgcatga catttgctaa cggcgcggta agatcggctt tgtggttgag tggtaaggaa
     8581 agtggtcttt ttgatatgcg agatgtgctt gatctcaata gtttgtaacc acaaaatatt
     8641 tgttatggtg caaaa
//
LOCUS       DAZGOB010000171         8618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGOB010000171 DAZGOB010000000
VERSION     DAZGOB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.171"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(239..445)
                     /locus_tag="ACHLIF_004529"
     CDS             complement(239..445)
                     /locus_tag="ACHLIF_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO9608400.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            758..847
                     /gene="kdpF"
                     /locus_tag="ACHLIF_004530"
     CDS             758..847
                     /gene="kdpF"
                     /locus_tag="ACHLIF_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO9608401.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            847..2520
                     /gene="kdpA"
                     /locus_tag="ACHLIF_004531"
     CDS             847..2520
                     /gene="kdpA"
                     /locus_tag="ACHLIF_004531"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO9608402.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLDQHYLPLNPQQLPGLSWDLA
                     LNAAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGSVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2543..4591
                     /gene="kdpB"
                     /locus_tag="ACHLIF_004532"
     CDS             2543..4591
                     /gene="kdpB"
                     /locus_tag="ACHLIF_004532"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO9608403.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSARGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4600..5172
                     /gene="kdpC"
                     /locus_tag="ACHLIF_004533"
     CDS             4600..5172
                     /gene="kdpC"
                     /locus_tag="ACHLIF_004533"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO9608404.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5165..7849
                     /gene="kdpD"
                     /locus_tag="ACHLIF_004534"
     CDS             5165..7849
                     /gene="kdpD"
                     /locus_tag="ACHLIF_004534"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO9608405.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLTDPAEEKAVVRYARE
                     HNLGKIILGRPASCRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7846..8523
                     /gene="kdpE"
                     /locus_tag="ACHLIF_004535"
     CDS             7846..8523
                     /gene="kdpE"
                     /locus_tag="ACHLIF_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO9608406.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1825 a         2209 c         2479 g         2105 t
ORIGIN      
        1 tcatccttgt tttatcgggt tatttgtctg cttccaggca aaaccctaca gatttataag
       61 aaagccttaa gtgagatatc tgtcagataa gaacaattag gaaaatattt atatatattt
      121 catgaatata tgtttcgatt ggtataacga tgtctaaata ttcctctgaa catcttcctg
      181 atacaaaaaa ccgccagcgt ttccgctggc ggttttcaat gtattcaaaa ctcagcaatc
      241 aataaaaatc accagttgcc ttttcggcct gatccatcca caccggttta tcgctggttt
      301 tcgaccagac gcgatgcagg tagctgtaaa agcgggcgcg gtctttccag aaaagcatga
      361 aaggcagcgc cagaacgccc gctgcaaccg cataagtacg gcgtaaaaag ataagccatg
      421 caggatattc tctgtagagt tccatacttt tctccccctt ttgtatctac ccggtgaatg
      481 gtaccggaaa aatgaatttg tttatctgat gaaaatagta ccgccttttg tgtaatttta
      541 ctactcatcc gaccacttat ttttgcttat tgatggttta tttacattca tcctgtaatt
      601 aagttacaca aaagttaaat taatactaaa cattagttaa atcatggctt ttgccatttt
      661 tatacttttt ttacaccccg cccgcagatt tttgcgaaat ctttgcagcc agaattctac
      721 ccttccggta tcacttttag gccactggag gtgcactgtg agtgcaggcg tgataaccgg
      781 cgtattgctg gtgtttttat tactgggtta tctggtttat gccctgatca atgcggaggc
      841 gttctgatgg ctgcgcaagg gttcttactg atcgccacgt ttttactggt gttaatggtg
      901 ctggcgcgtc ctttaggcag cgggctggcg cggctgatta atgacattcc tcttccaggt
      961 acagcgggcg ttgaacgtat actctttcgt ttacctggcg tctctgacca tgagatgaac
     1021 tggaagcaat atctttgtgc cattctcggc ctgaacatgc tggggctggc ggtgctgttt
     1081 tttatgttgc tcgatcagca ctatctgccg cttaatccac agcagttgcc ggggctgtcg
     1141 tgggatctgg cgctgaatgc cgccgtcagc tttgtcacga ataccaactg gcaatcttac
     1201 agcggtgaaa ccacgttgag ctatttcagc cagatggctg gcttaacggt gcaaaacttt
     1261 ctttctgccg ccagcgggat tgcggtgatt tttgccctca tccgtgcgtt tacccgccag
     1321 agcatgagca cactcgggaa tgcctgggtc gatctgctac gcatcacgtt atgggtgctc
     1381 gtccctgtgg cgttgttgat cgcactgttt tttattcaac aaggtgcgct gcaaaacttt
     1441 cagccttatc aggctgtgaa taccgttgaa ggagcgcaac agctgttacc catggggcct
     1501 gtagcttctc aggaagcgat caagatgctc ggtactaacg gcggtggctt ctttaatgcc
     1561 aactcgtcgc atccgtttga aaacccaacc gcactgacca acttcgtgca gatgctggcg
     1621 atcttcttga tcccaacggc gctgtgcttt gcctttggtg aagtgacggg cgatcgccgc
     1681 caggggcgca tgttgctgtg ggcgatgtca gtgatttttg tcatctgcgt aggcgtggtg
     1741 atgtgggcag aagttcaggg taatcctcat ctgctggcgc tcggcgcaga cagcagcatc
     1801 aatatggaag gtaaagagag ccgtttcggc gtgctggtca gtagcctgtt tgcggtcgtg
     1861 actacggcgg cttcctgtgg cgcggtgatt gcgatgcatg attcgtttac cgctctcggt
     1921 ggcatggtgc cgatgtggct gatgcaaatt ggtgaagtgg tgttcggcag tgtcggttct
     1981 ggtctttacg gcatgatgct gtttgtcctg ctggcggtgt ttattgccgg gctgatgatt
     2041 ggtcgtacac cggaatatct gggtaaaaaa atcgacgtac gcgagatgaa actgactgca
     2101 ctggcgattc tggttacccc gacgctggtg ctgatgggcg cggcgctggc aatgatgacc
     2161 gacgccggac gtagcgccat gctcaaccct ggcccgcatg gttttagcga agtgctgtac
     2221 gccgtgtcat ccgccgctaa caacaacggc agcgcctttg ccggattaag cgccaactcc
     2281 ccgttctgga actgtttact ggcgttctgc atgtttgtcg gtcgcttcgg ggtgattatc
     2341 ccggtgatgg caattgccgg ttcgctggtg agtaaaaaga gccaacccgc cagctccggc
     2401 acgctgccaa cgcacggccc gctgtttgtt ggcctgttaa tcggcaccgt gttgctggtt
     2461 ggcgcactga cctttatccc tgccctggcg cttggtccgg tggcggaata tctctcctga
     2521 tgatattgag tgagcactga atatgagtcg taaacaactg gcgctattcg aaccaacact
     2581 tgtcgttcag gcgctgaaag aagcggtgaa aaaattaaac ccgcaggcgc aatggcgcaa
     2641 tccggtgatg tttatcgtct ggatcggcag tctgctgacc acctgtatta gcatcgcgat
     2701 ggcaagcggt gcgatgcctg gcaatgcgct gtttagcgcg gcaattagcg gctggctgtg
     2761 ggtcaccgta ctgttcgcta atttcgccga ggcgctggca gaaggccgca gtaaagcgca
     2821 ggccaacagt cttaaagggg tgaaaaaaac tgcctttgcc cgcaagctgc gtgagccgaa
     2881 atatggcgct gcggcggaca aagttcctgc cgatcaactg cgtaaaggtg acatcgtgct
     2941 ggtagaagct ggcgatatta tcccctgcga tggtgaagtc attgaaggcg gcgcatcggt
     3001 cgatgaaagc gccatcaccg gggaatcggc accggtgatc cgtgaatccg gcggcgattt
     3061 tgcctccgtc accggcggca cgcgtattct ttctgactgg ctggtgattg agtgtagcgt
     3121 taaccccggt gagacatttc tggatcggat gatcgcgatg gtggaaggcg cacagcgacg
     3181 caaaacaccg aacgagattg ccctgactat cctgctgatt gctctgacca tcgtcttttt
     3241 actggcaacc gccacgctgt ggccgttttc cgcgaggggc ggtaatgccg tgagtgtaac
     3301 ggtactggtg gcgctgctgg tctgtctgat cccaaccact attggcggcc tgttgtcagc
     3361 gatcggcgtc gccgggatga gtcggatgct gggcgcgaat gtgattgcca ccagcggacg
     3421 tgcagttgaa gcggcaggtg acgttgacgt tctgctactg gataaaaccg gcaccatcac
     3481 actcggtaac cgtcaggcgt cggagtttat ccctgcgcag ggcgtggatg aaaaaacgct
     3541 ggctgacgcc gcacaactgg cttcgctggc tgatgaaacg ccggaaggcc gcagtattgt
     3601 gatcctcgcc aagcaacgtt ttaacctgcg cgagcgcgat gtgcagtcgc tccatgccac
     3661 ctttgtaccg tttactgcgc aaagccggat gagcgggatc aacatcgaca accgcatgat
     3721 ccgtaaaggt tctgtcgatg ccattcgccg ccatgttgag gctaacggcg gtcacttccc
     3781 taccgatgtt gatcaaaaag tcgatcaggt tgcgcgtcag ggggccacgc cgctggtggt
     3841 ggtggaaggt tcccgtgtgc tgggcgttat tgcgctgaaa gatatcgtca aaggcggtat
     3901 taaagagcgc ttcgcccagc tgcgcaaaat gggcattaaa acggtgatga ttaccggcga
     3961 taaccgtctg actgccgccg ccattgctgc ggaagcgggc gtcgatgatt ttctcgccga
     4021 agcgacaccg gaggccaagc tggcattgat tcgtcagtat caggcggaag gccgtttggt
     4081 ggcgatgacc ggcgacggca ccaacgatgc tccagcgctg gcgcaggcag atgtcgcggt
     4141 ggcgatgaac tccggtactc aggcggcgaa agaagcgggc aatatggtcg atctcgactc
     4201 taacccgacc aagttgatcg aggtggtaca tattggcaaa cagatgctga tgacccgtgg
     4261 ctcgctgacc accttcagca ttgccaacga tgtggcgaaa tacttcgcca ttattccggc
     4321 ggcgttcgcg gcaacgtatc cgcagttaaa tgcgctgaac atcatgcgcc ttcattcgcc
     4381 cgactccgcc attctcagtg cggtcatttt caacgccttg attatcgtct ttttgattcc
     4441 tctggcgtta aaaggcgtga gttataaacc gcttaccgct tctgccatgt tgcgccgtaa
     4501 cttatggatt tacggtctgg gtgggctgct ggtgccgttt attggtatca aagtcattga
     4561 tttactgctg accgtttgcg gtctggtgtg aggtttacca tgcgtggctt acgtccggca
     4621 ttatcgacat ttatctttct gttattgatt actggcggcg tttacccgct gctgaccacc
     4681 gtgctggggc aatggtggtt tccctggcag gccaacggtt cgttgattcg tgaaggtgat
     4741 acggtgcggg gttcggcatt aatcgggcag aattttaccg gcaacggcta ttttcagggt
     4801 cgcccgtcgg caacggcaga aatgccctat aatccacagg cttctggcgg gagcaatctg
     4861 gcggtcagta accctgagct ggataaacaa atagccgcac gcgttgctgc attacgggcc
     4921 gctaacccgg atgccagtac gaacgttccg gttgaactgg tgacggcatc ggcaagcggg
     4981 ctggacaata atatcacccc gcaagcggcg gtctggcaaa tcccacgcgt ggcgaaagcg
     5041 cgtaatctca gcgttgaaca gctcacgcaa ctgatcgcaa aatacagcca acaaccgctg
     5101 gtgaaatata tcggccagcc ggtcgtcaac attgttgaac tcaatctggc gctggataaa
     5161 cttgatgaat aacgaaccct tacgtcccga ccccgatcgt ctgctggaac aaactgccgc
     5221 gccgcatcgg gggaagctga aagttttctt cggtgcctgt gcaggcgtcg ggaagacctg
     5281 ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg ctggatattg tggttggcgt
     5341 ggtagaaacc catgggcgga aagataccgc cgccatgctt gaagggctgg ctgttctgcc
     5401 gcccaaacgc caggcgtacc gtgggcggca tatcagcgag tttgatctcg atgccgccct
     5461 cgcccgccgt ccggcgctga tcttaatgga cgaactggcg cacagtaatg cgccaggttc
     5521 tcgtcatcct aaacgctggc aggatatcga agaactgctg gaagctggca ttgatgtttt
     5581 cacaaccgtc aacgttcagc atctggaaag tctgaatgat gtggtcagcg gcgtcaccgg
     5641 aattcaggta cgggaaaccg tgcccgatcc ttttttcgat gccgccgacg acgtagtgct
     5701 ggtggacttg cccccggacg atctgcgcca gcggctgaaa gaaggcaaag tctatattgc
     5761 cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa ggcaatctga tcgccctgcg
     5821 tgaactggca ctgcgccgta ctgccgatcg cgtagatgag caaatgcgcg cctggcgggg
     5881 gcatcctggt gaagagaaag tgtggcacac gcgcgacgcg atccttttat gcatcgggca
     5941 taacaccggc agtgagaaac tggtccgcgc ggcggcgcgg ttggcctcgc gcctgggtag
     6001 cgtctggcac gcggtgtatg ttgaaacccc tgccctgcat cgcttaccgg aaaaaaaacg
     6061 tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg ggcgcggaga cggctacact
     6121 tactgatcca gcggaagaaa aagcagtagt gcgttatgcc cgtgaacata atctcggcaa
     6181 gattattctc ggtcgcccgg cctcgtgccg ctggtggcgt cgggaaacgt ttgctgaccg
     6241 actggcgcgc atcgcccccg atctcgatca ggtgctggtc gcgcttgatg aaccacccgc
     6301 ccgcacgatt aacaacgcgc cggatagccg ctcttttaaa gacaagtggc gggtacagat
     6361 tcagggatgc gtggttgccg ccgcgttatg cgccgttatc actttaattg ccatgcagtg
     6421 gctgatggcg tttgatgccg ccaacctggt gatgctgtat ctgcttggcg tggtggtggt
     6481 ggcgctattt tatggacgct ggccttcggt ggttgctacc gtcattaatg tggtgagttt
     6541 cgatctcttt tttatcgccc cacgcggcac gctcgctgtc tctgatgtgc aatatctgct
     6601 gaccttcgcg gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg cgggtgtgcg
     6661 ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat atgaaatgtc
     6721 gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc gccaccagcg aacaatttat
     6781 tgcctccacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg gtaaattgca
     6841 gccgttaaca catccgcaag gaatgacgcc atgggacgat gccatcgcgc agtggagttt
     6901 tgataaaggc ctgcctgcgg gtgcgggcac cgacacgtta cccggcgtgc cgtaccagat
     6961 tttgccgcta aaaagcggcg agaaaaccta cggactggtg gtggtggaac cggggaatct
     7021 gcgtcagttg atgatcccgg agcagcagcg cctgctagag acgtttacgc tgttagtcgc
     7081 caatgcgttt gagcggctga cgctgaccgc cagtgaagaa caggcgcgga tggcaagcga
     7141 acgtgaacag atccgcaacg ccctgctggc ggcgctttcg catgatttac gcacgccgct
     7201 tacggtgctg tttggtcagg cagaaatctt aacgctcgat ctggcaagcg aaggatcgcc
     7261 ccacgcccgc caggccagcg agattcgtca gcatgtgctg aacactaccc gactggtgaa
     7321 taatctactg gatatggcgc gaattcagtc cggcggcttt aatttgaaga aagagtggtt
     7381 aacgctggaa gaagtagtcg gcagcgctct gcaaatgctg gaaccgggtt tatcgtcgcc
     7441 catcaatctt tctctgccag aaccgctgac cttaatccac gttgacgggc cactctttga
     7501 acgggtgctg attaatctgc tggagaacgc ggtgaaatat gcgggtgcgc aggccgaaat
     7561 tggtatcgat gcccacgttg agggcgaaaa tctacaactg gatgtctggg ataacggccc
     7621 cggtcttccg ccaggccagg agcagacgat atttgataag tttgctcgcg ggaataaaga
     7681 gtcggcagta ccgggggtag ggcttggact ggcaatttgt cgggcgatag tggatgtaca
     7741 cgggggcact attaccgcgt tcaaccgacc ggaaggtggt gcctgttttc gtgttacact
     7801 tccccagcaa accgctcctg aacttgaaga ctttcatgag gatatgtgac aaacgttctg
     7861 attgttgaag atgaacaggc tattcgtcgc tttctgcgca cggcgctgga gggcgacggg
     7921 atgcgcgtct atgaggccga aacgctgcaa cgcggcttgc tggaagcggc tacccgtaag
     7981 ccagatttga ttattctcga tctcggcctg cccgatggtg atgggattga gtttatccgc
     8041 gacctgcgcc agtggagcgc ggtgccggtg attgtgcttt ccgcacgcag cgaagagagc
     8101 gacaaaatcg ccgcgctgga tgccggagcg gatgattatc tgagtaagcc gtttggcatt
     8161 ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc actccgccac cgccgcgccc
     8221 gatccgctgg tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt gattcaccgg
     8281 ggtgaggaag aggtgcatct cacgccgatt gagttccgcc tgctggcggt gctgctaaat
     8341 aatgccggga aagtgctcac ccagcgccag ttactcaatc aggtgtgggg gccaaacgcg
     8401 gtcgaacaca gtcactattt gcgtatttat atgggacatc tgcgacaaaa actggaacag
     8461 gatcccgctc gcccccgcca tttcattact gaaaccggta ttgggtatcg gtttatgcca
     8521 tgaatgttta ttcaataaat gcgcttaaat aattattaat ttattattaa acctgccaaa
     8581 aatattatta tttggcaggt ttaatttctt aacgcaaa
//
LOCUS       DAZGOB010000172         8518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGOB010000172 DAZGOB010000000
VERSION     DAZGOB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.172"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            210..1301
                     /gene="ompC"
                     /locus_tag="ACHLIF_004536"
     CDS             210..1301
                     /gene="ompC"
                     /locus_tag="ACHLIF_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO9608407.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1413..2468
                     /gene="apbE"
                     /locus_tag="ACHLIF_004537"
     CDS             1413..2468
                     /gene="apbE"
                     /locus_tag="ACHLIF_004537"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO9608408.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2542..3606
                     /gene="ada"
                     /locus_tag="ACHLIF_004538"
     CDS             2542..3606
                     /gene="ada"
                     /locus_tag="ACHLIF_004538"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO9608409.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3606..4256
                     /gene="alkB"
                     /locus_tag="ACHLIF_004539"
     CDS             3606..4256
                     /gene="alkB"
                     /locus_tag="ACHLIF_004539"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO9608410.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4332..5975
                     /gene="yojI"
                     /locus_tag="ACHLIF_004540"
     CDS             4332..5975
                     /gene="yojI"
                     /locus_tag="ACHLIF_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO9608411.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGQTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6193..7839
                     /gene="mqo"
                     /locus_tag="ACHLIF_004541"
     CDS             6193..7839
                     /gene="mqo"
                     /locus_tag="ACHLIF_004541"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO9608412.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(7988..8497)
                     /gene="eco"
                     /locus_tag="ACHLIF_004542"
     CDS             complement(7988..8497)
                     /gene="eco"
                     /locus_tag="ACHLIF_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO9608413.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         2045 a         2138 c         2301 g         2034 t
ORIGIN      
        1 tccgcaataa tcttaaaaag ttcccttgca tttacatttt gaaacatcta tagcgataaa
       61 tgatacatct taaaagtttt agtatcatat tcgtgttgga ttattctgca tttttgggga
      121 gaatggactt gccgactgat taatgagggt taatcagtat gcagtggcat aaaaaagcaa
      181 ataaaggcat ataacagagg gttaataaca tgaaagttaa agtactgtcc ctcctggtcc
      241 cagctctgct ggtagcaggc gcagcaaacg ctgctgaagt ttacaacaaa gacggcaaca
      301 aattagatct gtacggtaaa gtagacggcc tgcactattt ctctgacaac aaagatgtag
      361 atggcgacca gacctacatg cgtcttggct tcaaaggtga aactcaggtt actgaccagc
      421 tgaccggtta cggccagtgg gaatatcaga tccagggcaa cagcgctgaa aacgaaaaca
      481 actcctggac ccgtgtggca ttcgcaggtc tgaaattcca ggatgtaggt tctttcgact
      541 acggtcgtaa ctacggcgtt gtttacgacg taacttcctg gaccgacgta ctgccagaat
      601 tcggtggcga cacttacggt tctgacaact tcatgcagca gcgtggtaac ggtttcgcga
      661 cctaccgtaa cactgacttc ttcggtctgg ttgacggcct gaactttgct gttcagtacc
      721 agggcaaaaa cggtagcgta agcggcgaag gcatgactaa caacggtcgt gaagcactgc
      781 gtcagaacgg cgacggcgtt ggcggatcta tcacttatga ttacgaaggc ttcggtatcg
      841 gtgctgcagt ttccagctcc aaacgtactg atgatcagaa cagcccgctg tacatcggta
      901 acggcgaccg tgctgaaacc tacactggtg gtctgaaata cgacgctaac aacatctacc
      961 tggctgctca gtacacccag acctacaacg caactcgcgt aggttccctg ggttgggcga
     1021 acaaagcaca gaacttcgaa gctgttgctc agtaccagtt cgactttggt ctgcgtccgt
     1081 ccctggctta cctgcagtct aaaggtaaaa acctgggtcg tggctacgac gacgaagata
     1141 tcctgaaata tgttgatgtt ggtgcgacct actacttcaa caaaaacatg tccacctatg
     1201 ttgactacaa aatcaacctg ctggacgaca accagttcac tcgtgacgct ggcatcaaca
     1261 ctgataacat cgtagctctg ggtctggttt accagttcta atctcgattg atatcgaaca
     1321 aagggcctgc gggccctttt ttcattgttt tcagcgtaca aacacagttt tttggtgtac
     1381 tcttgcgacc gttcgcatga ggataatcac gtatggaaat aagctttacc cgcgtggcac
     1441 tgctggctgc cgcgctcttc tttgttggtt gcgatcaaaa accacaaacc gccaaaaccc
     1501 acgctactga agttaccgtt cttgaaggca aaactatggg taccttctgg cgtgccagca
     1561 tcccgggcat tgacgccaaa cgcagtgccg aacttaaaga aaagattcag acccagctgg
     1621 acgccgacga tcagctgctt tcgacctata aaaaagattc cgcgctgatg cgctttaacg
     1681 attcgcaaag tttatcgccg tggccggtaa gtgaagcgat ggccgatatc gtcaccacct
     1741 cgctgcgcat tggcgcaagg accgatggtg cgatggatat aaccgtcggg ccgctggtga
     1801 atctgtgggg ctttggcccg gaacaacagc cggttcaaat tccgagccag gaacagatcg
     1861 atgcgatgaa agccaaaacc ggcttacagc acctgacggt cattaatcag tcgcatcagc
     1921 aatatctgca aaaagaccta ccggatttat atgtcgatct ctccaccgtc ggtgaaggtt
     1981 atgcggcgga tcacctggca cgcttgatgg agcaggaagg gatttcccgc tatctggtgt
     2041 cggtgggcgg cgcgctgaac agccgtggta tgaacggtga aggcctgccg tggcgggtag
     2101 cgattcaaaa accaaccgat aaagaaaacg cggttcaggc tgtggtggat atcaacggtc
     2161 atggtattag cacctctggc agttaccgta actattacga actggacggc aaacgtcttt
     2221 ctcatgttat cgatccgcaa accgggcgtc ccatcgaaca caatctggta tccgtgacgg
     2281 tgattgctcc gacggcgctg gaagccgatg cctgggatac tggcttgatg gtactcgggc
     2341 cggagaaagc caaagaggtt gttcgccggg aagggctggc ggtttatatg atcaccaaag
     2401 aaggcgatag ctttaaaacc tggatgtcac cacagtttaa aagcttcctt gtcagcgaaa
     2461 aaaattaaag cgcaagattg ctggtttttg cgtgatggtg accgggcaga ctaaaggcta
     2521 tccttaacca gggagctgat tatgaaaaac gccacatgct taactgacga tcaacgctgg
     2581 caatctgtct tagcccgcga cccgaatgcc gacagcgaat tcgttttcgc cgtgcgcacc
     2641 acaggcatct tttgccgtcc gtcttgccgc gccagacatg ccttgcggga aaatgtctcc
     2701 ttctacgcaa atgcctgcga ggcactcgcc gccggatttc gcccctgcaa acgctgccaa
     2761 cctgataaag ccaatgccca gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg
     2821 ctggaacagg aaacgcctgt aacgctggaa gccttagccg accaggtggc gatgagtcca
     2881 tttcatctac atcggttgtt taaagcgact accggaatga cgcctaaagc ctggcaacag
     2941 gcctggcgcg ctcgccgttt gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct
     3001 attcttaacg ccggattccc cgacagcagc agttactatc gcaaagctga cgaaacgctg
     3061 ggcatgacgg ctaaacaatt ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg
     3121 gctgattgtg agctgggtcg ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata
     3181 ttgctgggcg atgatgacgc gacactaatc agcgagttgc agcagatgtt tcccgctgcc
     3241 gacaacgcgc ctgccgatct gatgtttcag caacatgtgc gtgaagtgat cgccagcctc
     3301 aatcaacgcg atacgccgct gacgttaccg ctggacattc gcggcactgc ttttcagcaa
     3361 caagtctggc aggcactgcg cacgatacct tgcggtgaaa ccgtcagtta tcagcaactg
     3421 gctaacgcca tcggcaaacc gaaagcggta cgggccgttg ccagcgcctg tgccgccaac
     3481 aagctggcta tcataatacc ctgtcatcgg gtggtccgtg gtgatggcac actttccggt
     3541 taccgctggg gcgtgtcgcg taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag
     3601 aggtaatgtt ggatctgttt gccgatgctg aaccgtggca agagccactg gcggctggag
     3661 cggtaatttt gcaccgtttt gcttttaacg ctgcggagca actgatccgc gatattaatg
     3721 acgttgccag ccagtcgccg tttcgccaga tggtcacccc cgggggatat accatgtcgg
     3781 tggcgatgac caactgtggg catctgggct ggacgaccca tcggcaaggt tatctttatt
     3841 cgcccattga tccgcaaaca aataaaccgt ggcccgccat gccacaaagc tttcatgatt
     3901 tatgtcaacg tgcggctacg gcggcgggct atccggattt ccagccagat gcttgtctta
     3961 tcaaccgcta cgctcctggc gcgaaactgt cgctgcatca ggataaagac gaaccggatc
     4021 tgcgcgcgcc gattgtttct gtttctcttg gcttacccgc gatttttcaa tttggtggcc
     4081 tgaaacgtaa tgatccgctc aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg
     4141 gcggtgaatc gcggctgttt tatcacggca ttcaaccgct gaaagcgggg tttcatccac
     4201 tcaccggcga ctgccgctac aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat
     4261 aagaattatt attgctgtgc gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc
     4321 gggttttctg catggaactt cttgtacttg tctggcggca gtatcgctgg ccatttatca
     4381 gtgtgatggc gctaagcctc gccagtgcgg cattaggcat tggcttaatt gcttttatca
     4441 atcagcgcct tatcgaaacg gcggatacca gtctgctggt attgccggag tttctgggat
     4501 tattgctgct gttgatggca gtcactctcg gatcgcaact ggcgctcacc actttggggc
     4561 atcacttcgt ttaccgactg cgtagtgaat ttatcaagcg gattctggat accaacgtcg
     4621 agcgcattga acaactcggt agcgcctcgt tgctggcggg attaaccagc gatgtgcgca
     4681 atatcaccat tgcttttgtg cgtcttccgg aactggtgca ggggatcatt ctcactatcg
     4741 gttccgcggc gtatctgtgg atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga
     4801 tggcgatcac catctggggt ggttttgtac tggtggcgcg ggtgtacaaa catatggcga
     4861 ccctgcgtga aaccgaagac aggctgtata cggattttca aactgtactc gaagggcgca
     4921 aagagctgac tctgaaccgg gaacgcgccg agtatgtgtt taacaacctc tacattcctg
     4981 atgcgcaaga gtatcgccac catattatcc gcgcagacac cttccatctt agcgccgtga
     5041 actggtcaaa catcatgatg ttgggcgcaa tcggcctggt gttctggatg gcgaacagtc
     5101 tcggttgggc tgataccaac gttgccgcga cctattcgtt gacgctttta ttcctgcgta
     5161 cgccgctgct ttcggcggtt ggcgcattgc cgacgctgct gacggcgcag gtggcgttta
     5221 acaagctgaa caaattcgcg ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt
     5281 ttcccaactg gcaaacgctg gagctgcgta acgtgacgtt tgcttatcag gataacgcgt
     5341 tttccgttgg tccgattaat ctcaccatca aacgtggcga gctgctgttt ctgattggcg
     5401 gcaacggtag cggaaaatcg acgctggcga tgttgttgac gggcttgtat cagccacaaa
     5461 gcggcgaaat cttgctggat ggcaaacctg tcagcggcga acaaccggaa gattatcgca
     5521 aactgttttc ggcagtgttt accgatgtct ggctgtttga tcaactgctg gggccggagg
     5581 gtaaacccgc taacccgcaa ctggttgaga agtggctggc gcagctgaaa atggctcata
     5641 agcttgagtt aagcaacggg cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac
     5701 gcgtggcgct gttgctggcg ctggcagaag aacgcgatat tatcctgctg gatgaatggg
     5761 cggcggatca ggatccacac ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc
     5821 aggagatggg tcaaactatt ttcgccatca gtcatgatga tcattacttt atccacgccg
     5881 accgcctgct ggaaatgcgc aacgggcaac ttagcgagct gacgggcgaa gagcgcgatg
     5941 ccgcttcgcg tgatgccgtt gcccggacgg cataatatta cgacgctgac ctggcggact
     6001 gctgccgtca ggtcaatatg ctaaaaatcc cttcatttct aataacccta taattaatca
     6061 acgaaattat aatgtttcta aaattagaat ataatttata aacattattt aaatgttgtt
     6121 acttaagtgt taccgttgat gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa
     6181 ggcattaaca agatgaaaaa agtgactgcc atgctcttct cgatggccgt ggggcttaat
     6241 gccgtttcga tggcggcaaa agcgaaagcg tccgaggagc aggaaactga tgtactgttg
     6301 attggcggcg gcattatgag cgccacgttg gggacctatt tacgcgagct ggagcctgaa
     6361 tggtcgatga ccatggtgga gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg
     6421 aataacgccg gaaccgggca ttctgcactg atggaactga actacacccc gcaaaacgcc
     6481 gatggcagca tcagtattga aaaagcagtc gccattaacg aagcatttca gatttcccgc
     6541 cagttctggg cgcaccaggt tgagcgcggc gtgctgcgta ctccgcgttc atttatcaat
     6601 accgttccgc atatgagctt tgtctggggc gaggataacg ttaatttcct gcgcgcccgt
     6661 tacgccgcgt tgcaacaaag ctcgctgttt cgcggtatgc gttactctga agatcacgcg
     6721 cagatcaaag agtgggcacc gttagtgatg gaagggcgcg atccacaaca gaaagtagcg
     6781 gcaacacgca cagaaattgg cactgatgtg aactatggcg agatcacccg ccagttaatt
     6841 gcttccttac agaagaaatc taacttctcg ctgcaactca gcagcgaagt ccgcgcccta
     6901 aagcgtaatg acgataacac ctggaccgtt accgttgccg atctgaaaaa cggcactgca
     6961 cagaacatcc gcgccaaatt tgtctttatc ggcgcgggcg gtgcggcgct gaagctatta
     7021 caggaatcgg ggattccgga agcgaaagac tacgccggtt tcccggtggg cggacagttc
     7081 cttgtttcgg aaaacccgga cgtggttaat caccatctgg cgaaggttta cggtaaagca
     7141 tccgttggcg caccaccgat gtcggttccg catatcgata cccgcgttct ggacggtaaa
     7201 cgcgtagtgc tgtttgggcc atttgccacc ttctcaacca aattcctcaa aaacggttca
     7261 ttgtgggatc tgatgagttc caccaccacc tctaacgtga tgccgatgat gcacgtcggg
     7321 ctggataatt tcgatctggt gaaatatctg gtgagtcagg tgatgttgag tgaagaggat
     7381 cgttttgaag cgttgaaaga gtactatccg caggcaaaaa aagaggactg gcgtttgtgg
     7441 caagcggggc agcgcgtgca gattatcaag cgtgatgccg agaaaggtgg cgtactgcgt
     7501 ctgggtactg aagtggtcag tgaccagcaa gggaccattg ccgcgctgct gggggcatcg
     7561 ccaggggcgt caaccgccgc gccgattatg ttggatctgc tggaaaaagt atttggcgat
     7621 cgcgtttcca gcccgcaatg gcaggctacg ttgaaagcga tcgttccgtc ttatggacgc
     7681 aagctgaacg gtgatgtagc ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg
     7741 gggctgaaat acgacaagcc gcaggcagca gatagtacgc caaaaccgca gttgaaaccg
     7801 caacccgttc aaaaagaagt ggcggatatt gcgttgtaat gatgtgccac atccggcatg
     7861 gtatgccgga tgtggcgtat gctgataaga cgcgccagcg tcgcatcagg caccggctcg
     7921 ggcgttagat gtcggatgcg gcgtgaccgc cttattcggc ctacggggtg ccgcacttca
     7981 cagcatttta gcgaactacc gcgttgtcaa ttttctcttc cgccttccag acgcggtact
     8041 tcacatctac attgtctggc gtatacacca cgatcggcag cttgctgttg taacgcagca
     8101 ttccagcatc gcccagatac gcggtgacaa atttcttctc tttcttgcca tccgggcagg
     8161 ccatcatcgt ggaaaccggc gagctaactt tatcaaagac ataataatca tagccccagc
     8221 cttccagcgt tttgctttcc agcttcccgc cgagacgatg caaattgcaa tcgacttcca
     8281 gcgtctgacc gattaacagt tctactttca gggtagattc atcttcttgc ggggttaact
     8341 gaatcacctg acgcttcatc cctttttcag cttgtggata aggcgcgatt ttttccagtg
     8401 gctggacgct ttctgccgcc caggcggaag tggtagcgaa agcggcaaac aatactgcag
     8461 gtagaatggt cttcattttt ttgctttcct tcttcaattt ataggttttc accgatga
//
LOCUS       DAZGOB010000173         8448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGOB010000173 DAZGOB010000000
VERSION     DAZGOB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.173"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..607)
                     /gene="yeeO"
                     /locus_tag="ACHLIF_004543"
     CDS             complement(<1..607)
                     /gene="yeeO"
                     /locus_tag="ACHLIF_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO9608414.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGNTKIPLLINGSLNI"
     gene            768..843
                     /locus_tag="ACHLIF_004544"
     tRNA            768..843
                     /locus_tag="ACHLIF_004544"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:801..803,aa:Asn,seq:gtt)
     gene            complement(881..1831)
                     /gene="cbl"
                     /locus_tag="ACHLIF_004545"
                     /pseudo
     CDS             complement(881..1831)
                     /gene="cbl"
                     /locus_tag="ACHLIF_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
     gene            complement(1933..2850)
                     /gene="nac"
                     /locus_tag="ACHLIF_004546"
     CDS             complement(1933..2850)
                     /gene="nac"
                     /locus_tag="ACHLIF_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO9608415.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3176..3251
                     /locus_tag="ACHLIF_004547"
     tRNA            3176..3251
                     /locus_tag="ACHLIF_004547"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3209..3211,aa:Asn,seq:gtt)
     gene            complement(3295..4242)
                     /gene="ldtA"
                     /locus_tag="ACHLIF_004548"
     CDS             complement(3295..4242)
                     /gene="ldtA"
                     /locus_tag="ACHLIF_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9608416.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMFYERW"
     gene            complement(4304..5383)
                     /gene="cobT"
                     /locus_tag="ACHLIF_004549"
     CDS             complement(4304..5383)
                     /gene="cobT"
                     /locus_tag="ACHLIF_004549"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO9608417.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCAAHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5395..6138)
                     /gene="cobS"
                     /locus_tag="ACHLIF_004550"
     CDS             complement(5395..6138)
                     /gene="cobS"
                     /locus_tag="ACHLIF_004550"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO9608418.1"
                     /translation="MSKLFWAMLSFITRLPVPRRRSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6135..6680)
                     /gene="cobU"
                     /locus_tag="ACHLIF_004551"
     CDS             complement(6135..6680)
                     /gene="cobU"
                     /locus_tag="ACHLIF_004551"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO9608419.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6832..7005)
                     /locus_tag="ACHLIF_004552"
                     /pseudo
     CDS             complement(6832..7005)
                     /locus_tag="ACHLIF_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(6984..7172)
                     /locus_tag="ACHLIF_004553"
                     /pseudo
     CDS             complement(6984..7172)
                     /locus_tag="ACHLIF_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(8169..8448)
                     /locus_tag="ACHLIF_004554"
                     /pseudo
     CDS             complement(8169..8448)
                     /locus_tag="ACHLIF_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2281 a         2249 c         1816 g         2102 t
ORIGIN      
        1 gaatattcag gctaccgtta atcagtagcg gtattttcgt attccctgca ccacgaagtg
       61 ccccgctacc aatcaaagtg atggcaactg ctggataact gagtaccgtc agctccagat
      121 aagtcaacgc cagtgcttta acttctgtcg tggcatcacc cgcgacgaaa tcaataattt
      181 gttcgccaaa atgatgaata agcgttgcca acagtacggc aaacaacgtc atgatcacca
      241 atgactgccg cgtcgccacc ctcgctcgtc gtcgatcccg cttaccgaga ctaaatgcca
      301 caacgacagt agtaccaaga tcgatagcag caaaaaaagc cataatgacc atattgaagc
      361 tgtccgccaa tcccacgccg gccatcgcat cttttcccag ccagctgacc agaaaagtgc
      421 tcaggacacc catcaacagg acacaggcat tctccatgaa gataggaaca gcaagcgggg
      481 ttatctcgcg ccagaacaac actttgtagc tcttgcgttt agcgtgccag cgagtgccgt
      541 gaacaacctg gcgtaaagca gaggagatat tcaaagccga ccttaattgc agaaagtgaa
      601 accacatttc aaataatgag ggagaatcag caaagctgca aagattttcg ccaacaaatt
      661 gtctgcaaat gcaacaaact gttgatagaa acggcaaaca gttgggaaat ttaaaaatcg
      721 ggtttgacaa aagatttttc gccgttaaga tgtgcctcaa cagcgattcc tctgtagttc
      781 agtcggtaga acggcggact gttaatccgt atgtcactgg ttcgagtcca gtcagaggag
      841 ccaaatttct gaaaagcccg cttttatagc gggctttttg ctatatctga taatcaattt
      901 cctcttcact gttctccatc acctggcgct tgatatcctc aactgacagt cctgcattac
      961 aaagttccag aaagcgccag acatagttac gttgaagttg ccctcgcttc agtcccaacc
     1021 atacagtatt ggcatcgaaa agatgccgcg tatccagacg gattaaattc tcttcctctt
     1081 gttcgccgct ggattgttcg gcaactaatc caatccccag cccaagagca acataggttt
     1141 taatgacatc agaatcctgc gcacttaata caatatctgc cagcaaacct ttgcgggcaa
     1201 atgcgtcatc aatacgtgag caccccgtaa tcccctatcg gtaagtgatt aacggccact
     1261 tcgctattga ttccagcgtc aacggtgtaa tttgcgtcaa gggatgatcg agtggaacaa
     1321 gcaaactatg gtgccaacga aaccacggga aggcgacgag atgcgggtca ttactcaaac
     1381 gctcgctggc gataccaata tcagcttcgc cattttgcaa caacgtcgca atttcctgtg
     1441 gcgtaccctg gattagctcc agccgaacct ccgggaaaag ttcgcgaaaa gctttaatga
     1501 cctcaggcaa gctataacgt gcctgagtat gcgtcgttgc aatagtgaga acgccagacg
     1561 tatcgttggt aaacaggtct gcaagccgac gaacattact ggcttcattc agaatacgtt
     1621 ctgcaatgac cagtaatgct ttgcccggtt cagtcatacc cagcagtcgc ttacctcgtc
     1681 gaacaaatat ttcgatgcca agttcatccc ccagttcccg aatatgacgg ctgacgccgg
     1741 actgtgaggt aaaaagcata ttcgcaacct ctgtcaggtt gtaatcctga cgtgcagcct
     1801 cgcggattat ctttagttgt tggaaattca cggtaaactc cgggcagctc agatttcccg
     1861 ttattgttaa agtctaatgc ccggcataac aaataataaa aacccgcatc ttattccatc
     1921 ccgatataac gcttagctca ccaattgcca ctgccttttt tccatcactg gagaactaat
     1981 cactgacatt aacaactctt tcactgcctg tgcctgtggc gataagttcg ctctggcggg
     2041 taaatttaat gacaaagaga gactcatgga aggcgtggta atgcgtgaca tccacccatt
     2101 tactgcgcca cataacgaac gcgcggccga ttcgggtaat actgcaacgc ccatgccgct
     2161 ggcaatcgct gcggtaagcg tggcaataga ctcaatttca ccaatgactt ttgccgtgag
     2221 ccgccgtagg gaaaaagcct catcaacacg aagtctaaca gcactgtaat cactggggag
     2281 aaagaggttc atttgcgcaa tagcattaac atcaacgctt tgccccgggc aatcttgagt
     2341 tcctaccagg aaaagatctt ctttcagcaa agcctgactg gataccccag ccacagggga
     2401 atgctcataa atcaccgcca tatcgagttg gtgatttatc agtttttcgt taagcactgc
     2461 accgctattt tcatgaagat agataacgat ctccgggaat tcagcgcgaa ccgcttgtaa
     2521 taagggcatg gtgatagatg acgcagcggt tcctggcgca aagccaatcg agacttgccc
     2581 cgataatgcc tgaccaacgt tatgcaccgc cagttgggcc tgctcacact gacgtaaaat
     2641 ggcccgcgca tgggtataga gaatttttcc ggcgtctgtt ggcgtaacgc cccgttttgt
     2701 gcgaatcaaa agttgttgat ttaactcacc ttccagtgtg gcaacctgct ggctgagcgc
     2761 tggttgtgcg atatgcaata cttcagcagc ctgagtcagg ctaccaatat ctacaatttt
     2821 tacgaagtat ttcaggcgtc tgaagttcat gttgcctccg gtttttgaga atcggcccaa
     2881 gtgccgccat tacttacaat cagactgcaa gatgcttgcc agttttattt tggcgttgat
     2941 gtacaagcta accaactgtc aaataagaga ttatgataga ttcgtcattg gctcttttca
     3001 ccagcagtcg cgttcccctg ccctataaaa ggagggtatg caccacgatg gttcattacc
     3061 aataagattg aaatctcatc actttgttga aattggcagc aaacaaacaa aaaaatgcat
     3121 ttcacccttt gacatcacca tgcactgcca ttaatatgcg ccccgttcac acgattcctc
     3181 tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt
     3241 cagaggagcc aaattcaaaa aagcctgctt tctagcagga tttttgcttt ctaattacca
     3301 acgctcataa aacatctgtc ttgaaccaga actaatttgc acaggcattc ccgatcgacg
     3361 ttgcaacgca gcatttgcgc gattcacatc aacttcttgc ccgttgataa acgcccgcaa
     3421 agatggggtt accggcaatg gcacttttcg gtcagactca tattctgcac gattgcgcga
     3481 cagcggctca tgaacttcca gccagttcga gccatctggt tcagtggtgt attttactgg
     3541 ctggtcgata atttgcacac gcgttccaac aggaacatta tcaaacagat atttgatatc
     3601 gtcattgcgc agacgaatac agccctgact tacccgaagt ccaataccaa aattggcatt
     3661 ggtaccgtgg atggcataca acctgccaat ataaatcgcg tacagcccca tgggattatc
     3721 gggccccgca ggaacaaatg cgggcaaact ctcccctcgt ttcgcatatt cgcgccgagt
     3781 gttcggcgtt ggcgtccagg ttggagcttc ttgtttacgt tcaacggtag tcacccagtt
     3841 acgcggggtt tctcgcccag cctggccgat accaatagga aagacttcca cagtattact
     3901 gtctggcggg tagtaataaa gacgcatctc agcgacgtta accacaatcc ctttacgaac
     3961 agtggcgggc aaaatcagtt gctgcggaat ggtgagttgc gagccagact tcggcaagaa
     4021 aacatcagcg cccggattcg cttccagcat gttacttaac ccttgcccgt attgtgcggc
     4081 aaaagtctcc agcggctggg tattgtgatc aggaacagtt acagtaagcg actgccccac
     4141 caaacggcta ccctctggag gtaatggata agttaccgcc aggctagtat ggctggcaaa
     4201 aagcagagca aatgagcaaa gaatatttac acgacgcatc atgtcccttt cctatgtcgc
     4261 gaaagctatc cgttaagtat agcttttatc agacttttcg tttttaactg ttcaaatcag
     4321 aagtcgtatt ccccggtaga acaatattac tggcagcaag ttcgcccatg ttgttatata
     4381 tcgcacaggc agcttcgatg atgggcatcg ccagagctgc gccactcccc tcacctaaac
     4441 gcatctccat attgagataa ggctccagcc ccaaatgcga cagcgctata cgcgcccctt
     4501 tttctgccga caggtgagaa ggaatgagat acggtttgat tgcaggagac atctggcagg
     4561 ctgcgagcgc agcagcataa gaaagaaatc catccagcaa cacgggtaaa ccgcaggaag
     4621 cagcgcctaa catcacgcca gccattccga ccaaatcaaa tccccccact tttgccagaa
     4681 catcaacgcc atcctgcgga tttggttgat tcaacgtaat cgcccggcgc acaacatcaa
     4741 ttttattagc cagtttatct gtcggcaggt ttgcgccaat cccaaccact tcttcaggat
     4801 cccggccagt gattgtgctg actattgccg ctgccggtgt cgtgtttgcc atccccagtt
     4861 cacctacacc aaacagcgtg acaccgtttt ttgccagctc ccgcgtataa catatgacgt
     4921 ccaaaagcaa cttttcagcc tgacggcgac tcattgccgg agctgaagca atattgccgc
     4981 tacctcgtgc gacacgcatg ttgataagcc cggggatagg ctcagcacta tcaataccaa
     5041 catcaactac gtggacgtta gcgcccgctt gtgctgccag cacacacacg ccggttgttc
     5101 cgcgggtcat attttcagcc tgaatggctg tcacttcttt tggggaaata gcgacccctt
     5161 cctcccagac gccgtgagcg gcacacataa ccagtaccgc ttttttgccc acatgcggta
     5221 tgccattcaa ccccggcatt cccgccagtt gtatggcaag cgcctccagc cttcccaggc
     5281 taccaacagg tttgagtaac ccgtcaatat gccgttgtgc acgcgacata gcggcaggat
     5341 cgatagcagg gatcgtattc agtaaatcgg caagtgtttg catgtcacgt ctctttatag
     5401 cagtgccagc aggaagacca gttcaccaag ctctatagca gctcccagcg tatcgccggt
     5461 ttgcccgccc agcgtacgtt tgagaagctg accgaggatg aaaatcgcca ccatcgtcac
     5521 caccatagca gccacaccat gcatacccgg caataatacg gcagcaaaaa tagcggctaa
     5581 gccgagtgtg acgcaggttt gtcgcccatc aattttgcca atgaacacat tgccaagccc
     5641 ttcttcccgg gcataacgat gacgatacat caataatgcg gcagtcccgc ggctgaccgc
     5701 acatgctgcc gccagcgatg caaggatcgg ttcgccacgc agagccaact cgcttaatac
     5761 cagaatcttt gccagtacaa caaaaatcaa tgccagaccg ccatgggtgc ctaaacggct
     5821 atcacgcatg atttccaaca tgcgatcgcg gctacgtgca gaaaatacgc catcacaggt
     5881 atcggcgagg ccgtctaggt ggaatccccc ggtcatcagc gccagcacaa gtacgctaaa
     5941 cagtgccgcc agtggtacgc cacaccatgc ctgcagcacc atgaagacca gcccgctaat
     6001 cgcgccaagc aataatccaa ttaaaggaaa agtaataata ccgcgagaat aatgctcgaa
     6061 atccagtccc tgggacctgc gacgcggtac aggcagacgc gtaataaatg agagcatcgc
     6121 ccaaaataat ttactcattt gatttttact ccaatacccg aaaccaccag ccatacttca
     6181 tttgccgcag cggccaactg ctgatttacc cgcccggcaa tatcacgaaa atgtcgtgcc
     6241 agacgactct ccggcacaat ccccattccc acttcgttag tcactaatac aacctttgcg
     6301 gggcaacgtt ggcaggcagc aatcaacgac tgaatctcag cattaatcgc ctgttccatc
     6361 gcctgataat cccattcatc agggtcttta tcgccgccat aatcaaacaa cagattagtc
     6421 accattgtgg taacgcattc aagcaacaca acctcattcg ggttaatgtc tgcatgaatt
     6481 aattcatcaa gatgttgcca gcgctccact gtgcgccagt gctccgggcg gccttgccga
     6541 tgatgttcta tccgtgcagc catctcatca tcaaggattt gcgaggtagc gatatacaga
     6601 acctgtgaag agtccccaat aagcgcctct gcgtggcgac tcttcccgct ccgtgcgccc
     6661 cccgtcacca aaatcatcat accgactcct gattcaaatg tatgatggta ttcattgaac
     6721 agaacaatgg atcccccacc gttgctgacc gcaaaacatt tacgcgatta atactgcgcg
     6781 taatataatt aaaaatccag cattctcaat ccattacgaa ataagatggc attacggaat
     6841 agccagactt tctcgtgtta acgtccgaca aaatgcgtca ttgacagcgg tttctgtgga
     6901 ttgacacatt tcattcagat aatgaattaa tgctactgcc ggaacaacca gcaaacgggc
     6961 attgtgttct gaaatccagg ctattgattc aacgtcagcg acagatctgc gctgtaaaag
     7021 aacttgtaaa acaacgtaat agaattatcc ggaatggtgg cgactatgca ctagaacacc
     7081 acaatttcgc tctctcggta aggcactgtc acagcattca acagaatgtg acttgccaga
     7141 tttattagcg ccaccagatg tttaaccggg taaccacgac ccagtcgggg atatattcca
     7201 tcttctcgct gacatcatca agaataaaaa ggtttatcac actcagagca acgttcttca
     7261 gcaggcggga taattcttca acatttagaa gatgtgttat taaaggcata caaactggac
     7321 gtattgtatt ttcttttggt ggttgaccta aggtagcagt ttatcctgat gcgctgagat
     7381 ttctgtaata tctacgtcaa cattctcctc gaatagaaaa tgcagccccg gcaagtgatt
     7441 cacatttttt aacaaaacgt tgttagcgtg ccattttcag aagctctcca agaaagcgaa
     7501 ttcttttctc aaattctgca ttctcagaca cagctttcta ctccatggta atggcccaaa
     7561 tacgaagttg ctcaggatca ttagtggtgc tgagaagtta aataaccatg cggagtacca
     7621 ctcgtcatca cgtatttata tatcatgaag ctattgttgt gttttgtaca tcagtagata
     7681 tattctgata tactcctttt gctagacata acctttcacc tgcttgcaaa gcttctgtgt
     7741 tctgacattg ccaaattgtt gcaattctgt atccagcctt ctttcagtca tagcttcggg
     7801 ccgcgataag actcactgat ctgaccctga ttcctcttgc agactttata gaccaattaa
     7861 aatgcagttt ctgcaggtca acgtctgacc atcattgtca tcactctggc cattagagta
     7921 accttctgca ttcatccttt tgtaaaaagt ttatgtcagt atcagcaatt aaccggacct
     7981 gatactgata tgagtcttac cgcatatacg gtcaatttca gcaattaatt acattatcca
     8041 cgccaaagtg tttgtcatcg caatgatggt accttctttc agacaccatt ttttcaactc
     8101 cgttttccac ggaccgcact cttatgtcaa gagtgcggtg atcctaccca cgtaatatgg
     8161 acacaggcct aagcgaggtt ctggttttca aattgttccg gactgaggcc gccacaccaa
     8221 ctgtgccgcc gccaccgatt gtaatcacat tcgatataat taaacaccgt tgcccgcatt
     8281 atttcccggc tgataaagtg ttctccatgg atacattcca ctttcagcga atgaaagaag
     8341 ctttccacgc agattatgcc gcttcagttg cgcctgataa tctgctgaac agtactggcc
     8401 tccacggtcc gtgtgaacga taacgttccg gggcctctta cgccgcca
//
LOCUS       DAZGOB010000174         8412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGOB010000174 DAZGOB010000000
VERSION     DAZGOB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.174"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            17..1591
                     /locus_tag="ACHLIF_004555"
                     /pseudo
     CDS             17..1591
                     /locus_tag="ACHLIF_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1560..3296)
                     /gene="aceK"
                     /locus_tag="ACHLIF_004556"
     CDS             complement(1560..3296)
                     /gene="aceK"
                     /locus_tag="ACHLIF_004556"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO9608420.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPHFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3577..4881)
                     /gene="aceA"
                     /locus_tag="ACHLIF_004557"
     CDS             complement(3577..4881)
                     /gene="aceA"
                     /locus_tag="ACHLIF_004557"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO9608421.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4911..6512)
                     /gene="aceB"
                     /locus_tag="ACHLIF_004558"
     CDS             complement(4911..6512)
                     /gene="aceB"
                     /locus_tag="ACHLIF_004558"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO9608422.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6781..7710)
                     /gene="metA"
                     /locus_tag="ACHLIF_004559"
     CDS             complement(6781..7710)
                     /gene="metA"
                     /locus_tag="ACHLIF_004559"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO9608423.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7867..8310
                     /locus_tag="ACHLIF_004560"
     CDS             7867..8310
                     /locus_tag="ACHLIF_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9608424.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2172 a         2079 c         2066 g         2095 t
ORIGIN      
        1 acaatgagat atttaaatga ttactcgtat ccctcgtagt tctttctctg caaatattaa
       61 taatacagcc cagacaaatg aacaccaaac cctgagtgaa ttgttttata aagaactcga
      121 ggataaattt tctggcaagg agctggcgac gcctctatta aaaagcttct cagagaactg
      181 tagacaaaat ggtcgtcata tctttagcaa caaggatttt gtcattaaat tttccacgtc
      241 cgtcttacaa gctgataaga aagaaattac gataattaat aaaaacgaaa acacgacact
      301 tactcaaacc attgccccaa tatttgaaga atacctaatg gaaattttac ctcaacgctc
      361 agacactctt gataaacaag aattaaacct aaaatcagat agaaaagaaa aagaattccc
      421 aagaattaaa cttaatggtc aatgttattt tccggggcga ccccaaaacc gtatagtatg
      481 ccgacacatt gctgcacaat atattaatga tatttatcag aatgttgatt acaaacccca
      541 tcaagatgat tactcttcag ctgaaaaatt tctcactcac ttcaacaaaa aatgcaaaaa
      601 ccagactttg gcgttgattt ccagccgtcc tgaggggcgt tgtgttgctg cctgtggtga
      661 tttcgggcta gttatgaagg catatttcga caagatggaa tcaaatggca tcagtgttat
      721 ggcagccata ttactggtgg ataaccatgc tttgacggtc cggctaagaa taaagaacac
      781 aactgaagga tgtacccatt acgtggtttc ggtttatgat cctaatgtca ctaacgataa
      841 aataagaatt atgagcgaaa gcaaagagga tattaaacac tattctctga tggattttat
      901 gaatgtagat tatagcctcc tgaaatggtc aaatgatcat attattaacc aatctgttgc
      961 aataattcca gcacttccga aagaacagct attgatgtta aaaggatctg tggatgaaat
     1021 aacccctcca ttatcaccag caacgatgaa tttgctaatg gcaattggtc agaatcacca
     1081 acttacgcaa ctgatgattc agctccagaa aatgccagaa ctacatagaa cagaaatgtt
     1141 gactgcctat aatagtggac atatgaacgt tattaatact atttttaacg cataacccac
     1201 tctgtttaat acgtttaaat tcgataaaaa aaatatgaag cccctcctcc tggcaaataa
     1261 ttctaatgaa tatcccggtt tgttttcagc gatacagcat aaacaacaaa atgttgtaga
     1321 gacggtttat cttgctttat ctaaccatgc acgcctgttt ggatttaccg ctgaagatat
     1381 tatggatttt tggcaacaca aagccccaca aaaatactct gcctttgagt tggcttttga
     1441 attgggtcac cgggttattg ctgaattaat ccttaataca ttaaataaga tggctgaaag
     1501 ctttggcttt acggataacc ctcgatacat tgcggagaaa aattatatgg aagctttact
     1561 caaaaaagca tctccccata ccgtacgcta aatctttgcc tgcgccgata cgcataaaca
     1621 tcttccacat gcccttcacg tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac
     1681 aggtcggcgt gcatctcttc aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg
     1741 cgaaactctt ccgggaaaac atcgcccggc gagacgctgt accacggttc gctggcaagt
     1801 tcgtcttccg gatagcgcgg cggcgggatg tcgcggaaat tcacttccgt catgtagcaa
     1861 atttcatcgt aatcataaaa aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac
     1921 agcatgtcgc caggaaaaat gttagcagcg gcaagctggc gaatagcgtt accgtattct
     1981 tcaatggcgt cgcgcaactg ctgaccttcc acttgttcca gccagatatt gagcggcacc
     2041 atccgccgct cgatatagag atggcgaatt acaatctgct caccaagatc ggtgattttt
     2101 tccgctgctt cctggagcag taattccatt aatgccgggg aaatatgccg cttctccagc
     2161 acaaagtttt caaactcctg ggtgtccgcc attcggccca cacggtcgtg ctctttcacc
     2221 agttgatagc aggcacgaac gtgagcggca gacatctctt tctgcggcgc gaacttgtct
     2281 ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa ataccaacat caccattcca
     2341 cgaatacccg gcgcttcaat gaactgctca ttacagccct gtagataaac gagatattcg
     2401 cggtagcttt cggttttggc gtgcttctgg cagccgatag ccatatacaa ttcagcagtg
     2461 gttttacccg gcagaatttc ccgtagccac tcgaccagcg ctgcgggcag cggtgcataa
     2521 accataaaat aagaacgcgc aaagccaaaa acaatgctcg cttcggcggt cgtcgtcagg
     2581 caggtatcaa taaataactc gccgtcgtcc gtctggtgga tcggcagtaa gaagggcaac
     2641 gtgccggaag gcgtgatcag tttgcccacc agccaagcgg ctttattgcg gtaaaacagt
     2701 tcgttcgcca cctgtaaatg actttccgcg aggttgtctg tccccagcgt ttccgtcaga
     2761 tggcgaatga tgtaatgaat gtcacggctt ttattctgcc agcgcaggcg cagcggcagg
     2821 tcgctgataa cgcgcatcag tagagattcc cagccgtgat cggggtgaaa gtctttcgcc
     2881 agcgggcggg gaatggtacg aaagcggcgc tctggctgag agctaaagat aaaaagccgc
     2941 tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca cggagttaaa aaagctctcc
     3001 gcaatctcga agtgcgggta atccggcaac agccgcgtgt aatgctcttt aacgcgcagt
     3061 aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc gcagttgctc cacgaccaga
     3121 ccaacgtgat gatcgtaaag atggatacgg tttttcatcg cctgctggac agcatgccag
     3181 tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt cgaggaatcg accatactga
     3241 gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt ccaggccacg cggcatttag
     3301 cgccctcatc aggagcagag aattgccgga tgcggcgtaa acgccttatc cagcctacgt
     3361 tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg catcaggcat cgtgcaccaa
     3421 ttgccggatg cggcgtaaat gccttatccg gcctacgttc ggcaacggct gtaggcatga
     3481 taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg gcgtgaacgc
     3541 cttatccggc ctacagtcag caacggttgt tgttgcttag aactgcgatt cttcagtgga
     3601 gccggtcagc gcggtgactg aagacgtgcc gccctgaata atagtcgtca ctttatcgaa
     3661 gtaacctgta cccacttcct gctggtgaga tacgaaggta tagccatctt tcgcggcggc
     3721 aaattccggc tgctgcactt tctcaacgta gtgcttcata ccctcgccct gggcataggc
     3781 gtttgccagg tcaaacatgt tgaaccacat gctgtggata cctgccaggg tgatgaactg
     3841 gaacttgtag cccatatccg acagctgctg ctggaagctg gcaatagttt tgtcgtcgag
     3901 gtttttctgc cagttgaacg acggcgagca gttataagcc agcagtttgc ccggatattt
     3961 cgcgtggata gcttgtgcaa agcgacgcgc cagttccaga tccggcgtgg aggtttcaca
     4021 ccagaccagg tcagcatatg gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc
     4081 cgcatgagta cggaagaagc cttcactggt acgctcgcca gtaataaatt cactgtcata
     4141 cgggtcgcaa tcggaggtaa tcagatccgc cgcatcggca tcggtacgag caaccagcag
     4201 ggttggaacg cccgtcacgt cagctgcaag acgcgccgcg accagtttct gaatagcttc
     4261 ctgagttggc accaaaactt tgccgcccat gtgaccgcat ttcttcactg acgccagctg
     4321 atcttcgaag tgaactgccg ctgcaccggc ttcaatcatc gctttcatca gttcaaaggc
     4381 attcaggaca ccgccaaaac cggcttccgc atcggcaacg atcggcagga agtaatcgac
     4441 atagcgcgga tcgcccggct caatgcctgc ggaccattgg atctgatcgg cacgacggaa
     4501 ggtgttgttg atccgctcca ccacagctgg caccgagttt gccggataga gcgactgatc
     4561 cggatacatg ctggccgcca ggttagcgtc cgccgctacc tgccatcccg acagatagac
     4621 tgcttcaata cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg caccgaggct
     4681 gttgatgtag ccttttttcg actcgccatg cagcagacgc cacattttcg ccgcgcccag
     4741 ttgcgccagg gtgcattcag gattgactga accgcgtaat ttcaccacat cttccgcgct
     4801 gtatgggcga gtaatgcctt cccaacgcgg ttgagtccac tctttctgta attcttcaat
     4861 ttgttgtgta cgggttttca tgtgcagatg ctccatagtt atgtggtggt ttacgctaac
     4921 aggcggtagc ctggcagggt caggaaatca attaactcat cggaagtggt gatctgttcc
     4981 atcaagcgtg cggcatcgtc aaaacgcccc tgggagaaac gttcttcgcc cagttcgctg
     5041 gcaatgactt tcatctcttc acccagcatc tggcggaaca aggctttggt caccggtttg
     5101 ccattgctca acgttttttg atgatggatc cactgccaga ttgaggtacg ggaaatttca
     5161 gccgtcgccg catcttccat caggccataa atcggcacgc agccgttgcc ggagatccat
     5221 gcttcgatgt actgcactgc cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca
     5281 ccatcacaag gtgccagcag ctgatcggca gtaatcggcg cgtcttgttc gcgcatcact
     5341 tcaagctgat ttttacggga gccgagaatg tcgttgaata ccgccattgc cgtatccgca
     5401 aggcctgggt gagcaatcca tgtgccatcg tgaccgttat tggcttccag cgctttatcc
     5461 gcttttactt tgttgagcac ctggttattg cgctcttcat ctttgctcgg aataaacgct
     5521 gccatgccgc ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcaa caggcgtgag
     5581 taagcattca ggaatggttt atccatcgtc actgcctgtc tgtctggcag gacgcgatcg
     5641 ggatagtttt tcaacgtttt gatatagctg aagatgtaat cccaacgacc gcagttcaga
     5701 ccaacaatat ggtcacgcag cgcgtgaagg atttcatcca tctggaacac ggcgggcagc
     5761 gtttcaatca gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg atcttctgca
     5821 tagctgaaga cttcgctcca ccaggccgct tcctgccagg actgggtttt cggcagatag
     5881 aaatagggac cgctgccctt tgccaacagt gcctgatagt tgtggaagaa atagagcgca
     5941 aaatcaaaca ggctaccggg gattgcctca ccacgccagg tgacatgttt ttccggcaag
     6001 tgcagaccgc gtacccgaca aatcaaaacc gctggattgg gcttgagctg ataaattttg
     6061 cctgcttcat tggtatagct gatggtgccg ttaaccgcat cgcgcaggtt aatttgcccg
     6121 tcgatcactt tgttccagtc tggtgccagt gaatcttcga aatcggccat aaagactttc
     6181 acattggcgt tcagtgcgtt gatcaccatc ttgcgctcaa ccgggccagt tatctcgact
     6241 cgacgatctt gtaagtccgc gggaatacca cgaattttcc agtccgtatc gcgaatggaa
     6301 gctgtttccg aaataaaatc aggcaaagtt ccgttatcaa tatcttgctg ctgctgaatg
     6361 cgcgctgcca gaagtttatt gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat
     6421 tctaccgctt cggcagtaag aatttgcttc tcctgctcgc catacggcct tatgaaagcc
     6481 agttcatcgg ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat ggacccgaaa
     6541 acttccccac tgtgaacgaa ggatcgttgt gcgcttttcg ttcagcacaa ctaagactac
     6601 tcatttaaaa tgcaaaatca aaaacaattt ccattttaat taaaaatact tgataactta
     6661 ttgataacaa tatgaataaa atttattcac ctcctgaggt gcgtttcgga aataaaaaag
     6721 gcacccgaag gtgcctgagg taaggtgctg aatcgcttaa cgatcgacta tcacagaaga
     6781 ttaatccagc gttggattca tgtgccgtag atcgtatggc gtgatctgat agacgtaata
     6841 gttgagccag ttggtaaaca gcaaattacc gtgactacgc cagctcgctc gcggtgtatt
     6901 ttgcggatca ttgtgcggga aatagttata cggtacttcc gggcctagtc cggcttccac
     6961 atcgcggaaa tattcctgcg ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa
     7021 ggcaatgcgc ttatctttgc tggcaaacag atatgcatcc ccttcttccg tctctgccag
     7081 aatttccaga tcggtgtaat cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg
     7141 tggtgccagg aatgaatcat caaagccacg cgtcagaagc gcatgaggat ggagaatatg
     7201 atgctcgtaa acgccagaga gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat
     7261 attgagcgcg gcctgtaccg cccagcagac aaacagcgtc gaggtgacgt gatctttcga
     7321 ccactccagc acctgtttga tctgcggcca gtaagcgaca tcattaaact ccaccaggcc
     7381 cagcggcgca ccggttacaa tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt
     7441 acagtagaag ttgttcagat gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg
     7501 caacagctga atatcgacct gcaaaggtga gtttgaaagc aggcgcagaa actgattttc
     7561 agtttcaatc ttcttcggca tcaggttaag gattagaacc ttaagtggac gaatttcctg
     7621 accagacgca cgagaagttg tcatcacaaa gacgttttct tcacgcaaga aattgacggc
     7681 gggtagctcg tccggcacac gaataggcat aacctgatta cctcactaca tacgcttata
     7741 cgtttagaca tccagatagc tgaagataac cagaaaattt gccaatgtcg agcctgcatg
     7801 ttgaaggtga gaaagtttca aggaaaaggc tacgttagaa tataagaatg acgaaaagga
     7861 gagaaaatgg ttattagtat tcgccgctca cagcatgagg aaggggagga actcgttgcg
     7921 atttggtgtc gatctgtcga tgccacccac gattttttat cagcagagta tcgggccgag
     7981 ctggaagagc tggttcgttc ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa
     8041 cgggatcagc cggttgggtt tatgttgtta agtgggcagc atatggatgc gctgtttatc
     8101 gatcctgatg tgcgcggctg cggcgtgggt cggatgctgg tggagcatgc gctctcgatg
     8161 gcaccggaac tgacaaccaa cgttaatgag caaaatgagc aggcggttgg gttctataag
     8221 aaggtgggtt ttaaggttac gggacgctct gaggtggacg atttggggaa accgtatccg
     8281 ttgctgaatc tggcgtatgt gggggaataa gccgcccctg ccgtgcctgc ccaggctgga
     8341 gaagacgcgc cagcgtcgca tccggcattt ttttctgcgc caatgcaaaa aggccatcct
     8401 gacggatggc ct
//
LOCUS       DAZGOB010000175         8177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGOB010000175 DAZGOB010000000
VERSION     DAZGOB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.175"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            16..1005
                     /gene="ldhA"
                     /locus_tag="ACHLIF_004561"
     CDS             16..1005
                     /gene="ldhA"
                     /locus_tag="ACHLIF_004561"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO9608425.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1116..1538
                     /gene="hslJ"
                     /locus_tag="ACHLIF_004562"
     CDS             1116..1538
                     /gene="hslJ"
                     /locus_tag="ACHLIF_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO9608426.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1535..1801)
                     /gene="ydbJ"
                     /locus_tag="ACHLIF_004563"
     CDS             complement(1535..1801)
                     /gene="ydbJ"
                     /locus_tag="ACHLIF_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO9608427.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2075..5599
                     /gene="nifJ"
                     /locus_tag="ACHLIF_004564"
     CDS             2075..5599
                     /gene="nifJ"
                     /locus_tag="ACHLIF_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO9608428.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     TWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            5966..7099
                     /gene="ompN"
                     /locus_tag="ACHLIF_004565"
     CDS             5966..7099
                     /gene="ompN"
                     /locus_tag="ACHLIF_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO9608429.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            7240..7674
                     /gene="uspF"
                     /locus_tag="ACHLIF_004566"
     CDS             7240..7674
                     /gene="uspF"
                     /locus_tag="ACHLIF_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO9608430.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT         2128 a         2025 c         1997 g         2027 t
ORIGIN      
        1 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
       61 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
      121 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
      181 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
      241 tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag aactggggct gaaagtagtc
      301 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
      361 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
      421 ggtctgaccg gatttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
      481 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
      541 tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
      601 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
      661 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
      721 gcattgattg attctcaggc agcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
      781 ggtatggacg tgtatgagaa cgaacgcgat ctgttctttg aagacaaatc caacgacgtg
      841 atccaggatg acgtgttccg tcgtctgtct gcctgccaca acgtgctgtt taccgggcac
      901 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
      961 agcaatctgg aaaaaggcga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     1021 gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct attcttaaaa
     1081 tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaactagcc gcgtttgttg
     1141 cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt acgccagaac
     1201 agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg accagcgata
     1261 aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat ttccggcagc atgtgtaacc
     1321 gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg ctggcaatga
     1381 cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc
     1441 tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac
     1501 agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag ctcccggcgg
     1561 caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca atcgccgtac
     1621 catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac
     1681 tactggacat cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc tgctgaacag
     1741 gttcactact acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca
     1801 tctcttcttc ctctgatctt caagccaaac gacaccgcca taaataatag gcagcacaga
     1861 gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt
     1921 ttttgccctc tgtttgatca aaacattcat tacgctgatg tgggggacac aaaagcgaaa
     1981 atgcagaaga aagccatttg ctaaaattga aggattacta ctgggcgcgc agcaatttcg
     2041 tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg gtaatggcgc
     2101 ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc atctacccta ttacccccag
     2161 ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttgaaga acgtttgggg
     2221 agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg
     2281 cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt tgctgctgat
     2341 gatcccgacg ctgtacaaac tggcaggcga actcacaccg tttgtcctgc atgtagcggc
     2401 acgtaccgtt gccacacatg cactctctat ttttggcgat cattccgacg tcatggcggt
     2461 gcgccagacg ggttgcgcga tgttgtgtgc ggcaaacgtc caggaagcgc aagactttgc
     2521 tctcatttcg cacatcgcga cgctgaaaag ccgcgtgcca tttattcatt tctttgatgg
     2581 tttccgcacg tcccacgaaa tcaacaaaat tgtcccgctg gccgatgaca cgattcttga
     2641 tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg gcactcaacc cggaacatcc
     2701 ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg aagccaccaa
     2761 cccatggtac gacgcggtct atgaccatgt tgaacaggcg atgaatgatt tctctgccgc
     2821 gacaggtcgt cagtatcagc cgtttgaata ttacgggcat ccgcaagcgg aacgggtgat
     2881 tatcctgatg ggctctgcca ttggcacctg tgaagaagtg gttgatgaat tgctaacccg
     2941 tggcgaaaaa gtcggcgtgc tgaaagttcg cctgtaccgc cccttctcag ccaaacactt
     3001 gctacaaact ctaccgggat ccgtacgtag cgtggcggta ctggacagaa ccaaagaacc
     3061 cggtgcccag gcagaaccgc tctatctgga tgtgatgacc gcactggcag aagcctttaa
     3121 taatggcaag cgcgaaactc tcccccgtgt cattggtggg cgctatggtc tttcatccaa
     3181 agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa
     3241 agcgcgcttt acggttggta tttacgatga tgtaaccaat ctgtccctgc cgttgccgga
     3301 aaacacccag ccaaactcgg cgaaacttga agccttgttt tatggccttg gtagtgatgg
     3361 cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt aattccacgc cgtggtacgc
     3421 acagggctat tttgtttacg actccaaaaa ggcgggcggc ctgacggttt ctcaccttcg
     3481 agtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt ttgttggctg
     3541 ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac ctggcggcat
     3601 tttcctgctc aacacaccgt acagcgcaga tgaagtgtgg tcgcgcttgc cgcaagaagt
     3661 tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg attaacgcgg cgaaaatcgc
     3721 ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt ttttccatct
     3781 gacgcaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga ttgccaaaag
     3841 ttacagtagc aaaggccagg atctggtgga acgcaattgg caggctctgg cgctggcgcg
     3901 tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg cacagcgcca atcgaccgcc
     3961 agtggtttcc gatgccgctc ctgatttcgt gaaaaccgta accgctgcga tgctcgccgg
     4021 gcttggtgac gccctccccg tttcggcgct gccgccagac ggctcctggc cgatgggcac
     4081 tacgcgctgg gaaaaacgta atattgccga agagatcccc acctggaaag aggaactctg
     4141 tacccaatgt aaccactgcg ttgccgcttg cccacactca gctattcgcg caaaagtggt
     4201 gccgcctgaa gcgatggaaa acgcccctgc cagcctgcat tcgctggatg tgaaatcgcg
     4261 tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg gaagattgca ccggttgtaa
     4321 cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagccatcaa
     4381 tatgatgtct cgcctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa
     4441 cctgccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat cgcagctgat
     4501 tacaccgctg tttgaatatt caggtgcttg ctccggttgt ggcgagacgc cgtatattaa
     4561 attgctgact cagctctatg gcgaccggat gttgatcgct aacgccaccg gctgttcttc
     4621 aatttatggc ggtaacctgc cctctacacc gtataccacc gatgccaacg gtcgtgggcc
     4681 ggcatgggcg aactctctat ttgaagataa tgccgaattt ggccttggtt tccgcctgac
     4741 ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg ataaaatccc
     4801 ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca gaggttcgtc gagaacaggt
     4861 cgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc tgcgtgacgc
     4921 agatgcactg gtggaaaaat caatctggct gattggtggc gacggctggg cttacgatat
     4981 cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca ttctggtgct
     5041 ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac cgctgggtgc
     5101 agtaactaag ttcggcgagc acggcaaacg taaagcgcgt aaagatcttg gcgtcagtat
     5161 gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc tgaaccagac
     5221 ggtgaaagcg attcaggaag cggaagcgta tccggggcca tcgctgatca ttgcttatag
     5281 cccgtgtgaa gagcatggtt acgatctggc actcagccat gaccagatgc gccaactcac
     5341 agctaccggc ttctggccgc tatatcgctt cgatcctcgt cgtgccgatg aaggcaaact
     5401 gccgctggcg ctggattcac gcccgccgtc agaagcactg gaagaaacgt tacttcacga
     5461 gcaacgtttc cgtcgtctga attcgcagca gccagaagtg gcagaacagt tatggaaaga
     5521 tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg gaaaagcgga
     5581 aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa aaaaaagccc
     5641 ggacgactgt tcgggcttgt ctttttatat gttggaaaat cagtggcaat gcaatggccc
     5701 aacagaaaat aaaatctgtg acaataaagg catataaccc gcgcagaata acgtatagaa
     5761 aattaaaagt ataattttta ttttgtatgt attatcttcg atacattcac cagacttatt
     5821 atcatttcag gagaattatt tgattaaggt tttacttaag gcgtaacaaa tgatatttat
     5881 cggctaactg aacttctcct ttaggatgtt ttcactcccc aaaatgggga taaaaggcaa
     5941 ataaaataac taaaggattt attcaatgaa aagcaaagta ctggcacttt taattcctgc
     6001 cctgctcgcc gcaggtgctg cacatgcagc cgaagtttat aataaagacg gcaacaaatt
     6061 agatctgtat ggcaaagttg atggcctgca ttatttttct gataattcag cgaaagatgg
     6121 cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc caaattaacg atcaacttac
     6181 tggctacggt caatgggaat acaatattca ggcaaacaac actgaatctt caaaaaacca
     6241 gtcatggacc cgtctggcat ttgccggact gaaatttgca gattacggtt ctttcgatta
     6301 cggacgtaat tatggcgtaa tgtacgacat cgaaggctgg accgatatgc tgcctgaatt
     6361 tggcggtgac tcttatacca atgcagacaa ctttatgact ggtcgagcca atggcgtcgc
     6421 gacttatcgt aatactgatt tcttcggtct ggtaaatggt ctgaacttcg cggtgcagta
     6481 tcaaggtaac aacgaaggtg ccagtaatgg tcaggaaggc accaacaacg gacgtgatgt
     6541 tcgccatgaa aacggtgacg gctggggtct ttccacaaca tatgatttag gcatgggctt
     6601 tagcgctggt gcggcataca cctcttctga ccgcaccaat gaccaggtta accatactgc
     6661 ggcgggtggt gataaagcag acgcgtggac tgctgggcta aaatacgatg ctaacaatat
     6721 ttacctggca accatgtatt cagaaacgcg taatatgacc ccgtttggcg acagcgatta
     6781 tgctgttgca aacaaaaccc agaactttga agtcactgca cagtaccagt ttgattttgg
     6841 tctgcgtccg gcagtctctt tcctgatgtc taaaggccgt gacctgcacg ctgccggtgg
     6901 tgcagacaac ccggcaggtg ttgatgataa agatctggtt aaatacgccg atgttggcgc
     6961 gacttactat ttcaataaaa acatgtccac ttatgttgac tataaaatca acctgttgga
     7021 tgaagatgac agtttctacg ctgccaacgg catctctacc gatgatattg tcgctttagg
     7081 tctggtttat cagttctaaa tcctcctgcc cgctgttatg gcgggctttt tctgcttatt
     7141 cttcctcctt tgatctaaat taaaaatgtg aactccgtca ttacacaaaa agtgtcatct
     7201 ggcgttacac tttatgcgga tactaaaaca ggaggtttta tgaacagaac gattcttgtc
     7261 cctatcgata tttccgattc agaattaact caacgcgtga ttagccacgt tgaggcagag
     7321 gcaaagattg atgatgcaga ggttcatttc ctgacggtaa taccttcact gccctactat
     7381 gcctctctgg gtttagcgta ttccgcagaa ttaccggcaa tggatgacct gaaagcggaa
     7441 gccaaatcgc aactggaaga gatcattaaa aaatttaaac tgccaaccga cagagtgcat
     7501 gtccatgttg aggaaggctc gccaaaagac cgcattctgg aattggcgaa gaagatcccc
     7561 gctcatatga tcatcattgc ttcccatcga ccggatatca ccacttatct gctcggttct
     7621 aacgccgcag ctgtagtgcg tcacgcagag tgctccgtgc tggttgtgcg ctgacactaa
     7681 cgcccgcaca ttgctgcggg ctttttgatt catttcgcaa atgtgctgac attttcccct
     7741 ctaatccgta ccatacacgc cacagttttt atatcagatt tcttatgctg ggcgttccgg
     7801 catgacgcat actcttctga tgccatataa cgaattgagt cgcttttaaa tgtcgcaaaa
     7861 tcaagaaatt agtaagaaag aacaatacaa cctgaacaag tcagagtaaa aaccgcaact
     7921 atctgacgaa caataacttt tatgtttttc aatatgttag gcaggattaa caactacaat
     7981 aaagcacaat aatctacact ttttttgccc catttttgcc ccttttgcgg ccttttgtcc
     8041 catttttgcc acagtaaaaa tctataacta atcaatctgt gatctgtaaa ttgcgcccat
     8101 gatttctttc caatagaatc agttccggct ggaaatcatt agatactcgc actatcgaaa
     8161 agcttaccag ccaaccg
//
LOCUS       DAZGOB010000176         8136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGOB010000176 DAZGOB010000000
VERSION     DAZGOB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.176"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..6583
                     /gene="efa1"
                     /locus_tag="ACHLIF_004567"
     CDS             <1..6583
                     /gene="efa1"
                     /locus_tag="ACHLIF_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO9608431.1"
                     /translation="HLSLQSSQEANEFLEISTFIYENDFLREKIEAVKNKMNSHELYF
                     EKIKKEQNTWQDLSTKEQKLQLIKALKEISGNTEKDSHYDRLLDAFFKKHNENIHNKI
                     QRIKDEFKEYSRVTIHNIDKVIFKGQTLDRLYHEGYVFSDINTLSRYTLHGLGITGVH
                     TEENLLPAPSSSLINILKEHYNEDEISAKLPLAYDYILNKKESSSIPVEILNKLSELP
                     PHELLTPVLGQSVNPLGMGYSSDNGKITEQVIVSGADGFDNPISGLIYTYLEDLYNIH
                     VRMREGTLNSQNLRQLLENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGLPT
                     IADATLSLLSVGLPGTGKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSA
                     PVVSSLHFIAEHAKYTLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRC
                     MGLALLYLQTEDTAHYSILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEML
                     QYQGNKYITNESLLHKTAWNQERITLLFNEKGVKRALISTPNHTLVLQQLEDIYRLTD
                     PNFGHADFLSPIDALKFIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESDMVWNKLL
                     PENDAGLSTRIQHTTTDRLANLAEPVAVAGISLPVKTLYDIGATLDGRRITSPPTSEQ
                     IPSLRLNGDVLNDYLSRTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRGTQDDLVS
                     SQTRLQRQATRVKQQLAGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSGRFNLQIR
                     DGETLHTTSVEVPEVVSRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYARLLQQGHE
                     DSTLAKINLAMDIKQLSEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGMRSVATRT
                     GGTMGKALSASARVLELPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQIAFDSIS
                     LGLTAASVAFPPLIIATGPIAAIGMGASSIARNVARKEERHTQWLEYKKFLTDGSKHI
                     VVASPERGLLDFSGNKVFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLGDWQIREK
                     VGYANSISPYSSLAHGYANSKWPQTIPKIPSGEYDTIILGYGHQYQANTEIEYLSNWI
                     VWREAVPDSTSRHKRPPLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTEQAISLKD
                     YKFILRGGSGGLAVQVGGAGYYDIDANPVAKENTLSFRGLPEEFPLAFDLSKQTQSVM
                     LKTTDGEVPVMTITQKGINTLVGTTAGENWLTGNDKDNTFHTSSGGGTVISGGGNNRY
                     IIPRDLKTPLTLTLSNSSVSHEILLPETTLTELKPVAFELSLIYWAGNNIKVQLEDET
                     QLNRFAGNFRGHTSDGITLEAVSQENGIQLGVSSCDVQRWQAVYPEENNRPDAILDRL
                     HDMGWSLAPEVRFRGGETSASYDPLNRQLVYRLQERYSEFRLTGSRHYTTAVTGTPGS
                     RYIITGPDTAQISPAQIILAGDNDHPETIDLLEASPVLVEGKKDKNSVILTIATIQYS
                     LQLTISGIDESLPGTTRVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRLEN
                     LMALNQVMTFLPRVSGSAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEGEPTVPVN
                     ISDLSIPPYSRLYLIFEGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQEHIIVKP
                     DNEAPSLILSEFRRFTISSDKKFSLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGSLR
                     LTFRDFFTEVMDTTDREILEKELRPILIGDTHRFINAAYKNHLNIQLGDGVLNLADIV
                     AEYARIQKEETSKILYQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGEPFPTFHPWYTD
                     DLSGRYKSVPMARKADTLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKYNL
                     SILSEIPQNNNTVVHSILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFINGEQMLSY
                     DSHSSGRVYSREEYMMWELQQRVSEASSARTQDYWLMDAAVRNGEWKITPELLRHTPG
                     YIRSTVSKWSRGWLKTGTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGMAG
                     ADIADNAPGETRCTLRPGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNGDN
                     LFN"
     gene            6755..7105
                     /locus_tag="ACHLIF_004568"
                     /pseudo
     CDS             6755..7105
                     /locus_tag="ACHLIF_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7527..8136)
                     /gene="espF"
                     /locus_tag="ACHLIF_004569"
                     /pseudo
     CDS             complement(7527..8136)
                     /gene="espF"
                     /locus_tag="ACHLIF_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
BASE COUNT         2445 a         1812 c         1834 g         2045 t
ORIGIN      
        1 tcatcttagt ttgcaatctt ctcaggaagc aaatgagttt cttgaaatat ctacttttat
       61 ttatgagaat gatttcttga gagaaaagat tgaagcagta aaaaacaaaa tgaattctca
      121 cgaactttat tttgaaaaaa taaaaaaaga acaaaacaca tggcaggatc tgtccacaaa
      181 agaacaaaaa ttacagctta ttaaagcatt gaaagaaatt tcaggaaata cagagaagga
      241 ctctcattac gatagacttc ttgatgcttt ttttaaaaaa cataatgaaa atattcataa
      301 taaaatacaa agaataaaag acgagttcaa ggaatactcc cgtgtaacca ttcataatat
      361 agataaagtt atatttaaag ggcaaacact ggatcgtctt tatcatgaag gatatgtatt
      421 ttctgatatc aataccttgt ctcgttatac actacacgga ctaggaataa ctggtgtaca
      481 tactgaagaa aacctgctac cagctccttc atcgtcctta attaatatat tgaaagaaca
      541 ttataatgaa gatgaaatta gtgcgaaatt accactagca tatgattaca ttttaaataa
      601 aaaagaatca agctctattc ctgttgaaat tttgaacaaa ctttcagagt taccaccaca
      661 tgaactactc acacctgttc ttggccagag tgttaatcct ctgggcatgg gctactcatc
      721 cgataatgga aaaatcacag agcaagtaat agtcagtgga gctgatggat ttgataatcc
      781 catatctgga cttatatata cctatcttga agatctatat aacatccatg taaggatgcg
      841 agaaggtaca ctaaattcac agaatcttcg tcagcttctg gaaaactctg tttcttcatg
      901 ctttttgact gagcaaagta ttaataaatt acttagtgag gcagaaaaaa ggccttatca
      961 gtctttaaca gaaatacatc agcatctcac aggattacca actattgccg atgcaaccct
     1021 ttcattactt tctgttggat tacctggtac gggaaaacta ttgcgcaggg agcaggacta
     1081 tgggcgtcca ccagttacag caattcagga ttccacattt gtactccctt ataatttcaa
     1141 aggtattggt tttaacgata acattatatc ctctgcacct gtagtctcct cattacattt
     1201 tatcgctgaa catgcgaaat atactttgtt gtcatggcct gagttttatc gtcatcatgc
     1261 acagcgatgg ttcgaaatgg ctaaaggata tggaagccag aatattgatt ttcaccctca
     1321 gtctctattg gtaacccaag aaggacgctg tatgggatta gccttacttt atttacagac
     1381 tgaagatact gctcattata gcattctcca ggaaaaccta atgactgtga gtgcacttca
     1441 tcagaccagt aatcgcgata agttgccact gtccaaagat gataattcct taatgacaag
     1501 aacttatagt ctgattgaaa tgctacagta tcagggaaac aaatatatta ccaacgaatc
     1561 gctactacat aagaccgcat ggaaccaaga aagaataact ttattattca atgaaaaagg
     1621 agttaagcga gccctgataa gcacgcctaa tcatactctg gttctgcaac aactggagga
     1681 tatttaccgg ctcactgatc caaattttgg gcatgcagat ttcctttcac ctatagatgc
     1741 tctgaagttt attgaggcta tgatacaatt aactccaaca cttcaggaat attatggcct
     1801 attaaacaaa gacattaata aacatataca agtacattat gccgaatcag atatggtctg
     1861 gaataagctt ctgccagaaa atgatgctgg actgagcacc agaattcagc acaccaccac
     1921 cgaccgtctg gcgaatctgg ctgaaccagt cgctgttgca ggtatctccc tgccagtaaa
     1981 aacactttat gatatcggag ccacccttga cggtcggcgc atcacctctc ctccaacatc
     2041 ggagcaaatc ccttctctgc gtctcaacgg tgatgttctg aatgattatc tgtcccgcac
     2101 agttctgact ccagaacagg ctgataacat aagaaaaata ctgcacactc agggaatacg
     2161 cagcggtacc cgtcccatag atccggagat gattcgtggg acgcaggatg acctagtttc
     2221 gtcacagact cgtctgcaaa ggcaagcaac acgggttaaa cagcaactcg ccggcgtact
     2281 tgatactctg caacagcact tccagaacat tccacgttca tccggtcgtc atctttctgt
     2341 agagaatatt gagctggctg atatcggaag tgggcgtttc aaccttcaaa ttcgagatgg
     2401 agaaacattg cataccactt ctgtggaagt accggaagtt gtgtcccgtt ttcagaaact
     2461 ttctaccatg ctttcagccc tgcctgccag tggaatcatg gatttcgacc tcggcatgag
     2521 tgtggtcgga gtcgtccagt atgcccgcct gctacagcag gggcacgaag acagtaccct
     2581 agccaagata aatctagcta tggatatcaa gcaactttcc gaagcaactc tcggcagcat
     2641 gattcagatt gccgggaata agtttctcaa tacagaagga atccaggggt tcagactgga
     2701 aagcgccgtc gctgaaggta tgcgctcagt agcaacccgt accggaggca caatggggaa
     2761 agccctttct gccagtgccc gtgttcttga actgcctgta ctggaaacag ttctggggac
     2821 atggaacctg tacaacagcg tcattcagct ccagcaagct acatcttatt ctgagacaat
     2881 ggctgcccgg gtacagattg cgtttgattc catttctctg ggattaactg ccgcttcggt
     2941 agctttcccg ccactaatta ttgccactgg ccccattgcg gctattggta tgggagcttc
     3001 cagtattgca cgtaatgtgg cacggaaaga agaacggcat acacaatggc tggaatataa
     3061 aaaattcctg actgatggca gtaaacacat tgttgtggcc tctccggaaa gaggtctgct
     3121 ggatttctcc ggaaacaaag tttttggaaa aatggtgctg gatctgcgtc agtctcctcc
     3181 tctcttgcat ggagaaagct cttttaacgc tgaccgcaaa atcggtcatc gtccggatct
     3241 gggggactgg caaattcgtg agaaggtggg gtatgccaac agtatcagtc cctactcttc
     3301 cctggcgcac ggttacgcca acagtaaatg gccacaaaca ataccgaaaa ttccctcggg
     3361 agaatatgac accataattc tgggctacgg tcaccagtat caggccaata cggaaataga
     3421 atatctgtct aactggattg tatggcggga agccgtacca gacagtactt cccgccacaa
     3481 acgtcctcct ctggaggttc ttaatagtca gtgtactgtg atagctggag agcgtaaaac
     3541 cacagtactt cccctgagag tgctcagcga tctgacaccg gaatgcacag aacaggctat
     3601 atcgttaaaa gattataaat tcatactgag agggggaagc ggtgggctgg ctgttcaggt
     3661 cggtggcgcg ggatattatg atattgatgc aaatcctgtg gcaaaagaaa atacgctctc
     3721 ttttcgcggg ctaccggaag agtttccgct cgcctttgat ttatcaaaac aaacacagtc
     3781 ggtcatgctg aaaacaacgg acggtgaggt gccggtaatg accatcaccc agaagggaat
     3841 aaacaccctg gtcggtacaa cggccggcga aaactggcta acgggtaacg ataaggacaa
     3901 taccttccat acgagttctg gtggcggtac tgtcatctct ggtggaggga ataaccgcta
     3961 tattatcccc cgcgatttaa aaactccgct gacactgacg ctgtctaata gctcggtctc
     4021 tcacgagatc cttctgccag aaacaactct gacagaatta aaacctgtcg cctttgagct
     4081 gagtctgatt tactgggccg ggaacaatat aaaggttcag ctggaggatg agacacaact
     4141 gaaccgtttt gccggaaact tcagggggca taccagtgat ggcattactc tggaggcggt
     4201 ttcccaggaa aatggtattc aactgggggt ttcatcatgt gatgttcaac gctggcaggc
     4261 tgtttatccg gaagaaaaca acagaccaga cgccatactg gacaggctgc atgatatggg
     4321 atggagcctg gcccccgagg tcaggttccg gggaggagaa acatctgcca gctatgaccc
     4381 cctgaaccgt cagctcgttt accggcttca ggagcgttac tcggaattca ggctgaccgg
     4441 cagtcgccat tacaccacgg ctgtaaccgg aacccccgga agccggtaca ttattacggg
     4501 gccggataca gcacagatat caccggcaca aatcatactg gccggtgata atgaccatcc
     4561 ggaaacgatt gatttgcttg aggccagtcc tgttctggtt gaagggaaaa aagacaaaaa
     4621 cagcgtgata ttaacgattg ctacgattca gtattccctt caactgacaa tatccgggat
     4681 cgacgaatcg ctgcccggga caacccgtgt ggcaattcag cctcaggata cccgtttact
     4741 gggtgacgta ctccggatct taccagataa tggtaactgg gtggggattt tccggagtgg
     4801 tcatacacca acggtaaacc ggctggaaaa tttgatggca ctgaatcagg taatgacgtt
     4861 cctgccccgg gtatccggaa gtgcagagca ggtattatgc ctcgaaaacc taggtggcgt
     4921 aaggaaaaaa gtggaggggg agttactgtc agggaagctg aaaggtgcgt ggaaagccga
     4981 aggtgaacct actgttccgg taaatatctc agatctaagt atcccaccct attcacgtct
     5041 gtatctgatt tttgaaggga aaaataatgt gttgctacgc agtaaagtac atgcagctcc
     5101 gttgaaaata acatccgcag gagagatgca gttatctgaa aggcagtggc aacagcagga
     5161 acatattatt gtcaagcccg acaacgaagc cccctcatta atactcagtg aatttcgtcg
     5221 tttcactatt tcatcggata aaaaattttc tttaaaactg atgtgccatc aggggatggt
     5281 ccgcatcgac cgcagatcat tatcggtcag attgttctat ctgcgtgaac agccaggtat
     5341 cggcagttta cgtctgacgt tcagagattt tttcacagaa gtgatggata caacggacag
     5401 ggaaattctg gagaaagagc tgagaccaat tctgatagga gatacacacc gctttatcaa
     5461 cgctgcatac aaaaatcatc tgaatatcca gttaggagat ggcgttctga atctggcaga
     5521 cattgttgcg gaatacgccc gtattcaaaa ggaagaaaca tcaaaaatat tgtatcaata
     5581 tcaaggtgcc atgaaaaaaa aaacagatgg accatctgtg gtagaagatg ccattatgac
     5641 cactactgtc acaacagatt caggtgaacc attccctacc ttccacccgt ggtatacaga
     5701 tgatttatca gggcgttata agagcgtacc tatggcaaga aaagcagata ctttgtatca
     5761 cctgacacca aaaggtgatc tacagataat atatcaggta gctacaaaaa tggtgaatca
     5821 ggcgatgatt gtatccctgc caaactaccg acacgagtgg gaaaaatata atttaagcat
     5881 cttatccgaa atccctcaga acaataatac tgttgtacat tcaatcctca gggttaatgg
     5941 ccccacaatg caggtgcgca caattgacta cagaggaacg gatgaaaaca atcccatagt
     6001 atctttttca gatacaacct tcatcaatgg tgaacagatg ttgagttatg actcgcattc
     6061 atcagggcga gtctattcca gagaagaata tatgatgtgg gaattgcagc aacgggtatc
     6121 agaagcttcc agtgcccgga cacaggatta ctggctgatg gatgcagcgg taagaaacgg
     6181 agaatggaag atcacaccag aattattacg tcacacaccg ggatatatcc ggagtacggt
     6241 atcgaaatgg tccagaggat ggctgaaaac cggcacaata ctccagactc cagaagacag
     6301 aaatacggat gtatacctga ctaccataca gaacaatgta tttagtcgtc aggggggcgg
     6361 ctaccaagtg tattatcgga ttgatggaat ggctggtgcg gatatagcgg ataatgcacc
     6421 aggggaaacc cgctgcaccc tcaggcccgg aacatgtttt gaagtgacaa gtgtggatga
     6481 aaggcattat gagtggaata tcatttatgt cacgctgaaa acctgtggct ggagccgaaa
     6541 tggccaaagc aaaacgccga atggtgacaa cctttttaac taattaaaat ctctgttaaa
     6601 gatgtataac ccggcatcta tgtgctcacc agcatataga tgcaatgtga ttcccctgat
     6661 ggtcgtatca gagatggtat gcgagcctgt acatagattt gtgtaattgc ctgattttga
     6721 tatgttcaat ccaacatcaa aagcaggtta atttatggac gaaaaacagt tgcaggctct
     6781 ggctaacgaa caggccaaaa atctcaaaac ccctgaagat ctcagccagt tcgatcgcct
     6841 gctgaagaaa atcagcgttg aggcagctct caacgctgaa atgtcccacc atctgggcta
     6901 cgataaaaac cagcctaaac cgggtgccaa ctcccgcaat ggctattcca caaagaccgt
     6961 tatcaccggt gatggccccc tggaactacg cactccgcgc gatcgtgatg gctcttttga
     7021 accgtaactt gtgaagaaaa accagacccg gatcaccggg atggataacc agatcctgtc
     7081 gttgtacgcc aaaggcctga caaccgtaag cgtacagcct gaaccgtctg gtcagaatct
     7141 gacgaattag agtaagctgg acatccccag atatattatg gctgccagcg tcatatcggc
     7201 ataaatagag atatccctta acgctaacca gaaaaaaata cctataagag cctgtacata
     7261 gagttgtgtc gttgcctgat gttgatatgt caaatccaac atcaaatagc ggtaaattta
     7321 tggataaaaa attacaggct ctggttaacg aaacaccccc ctcttgctct attcccctct
     7381 ttcatcattt attacaatat ttatttcaat aaaaaccgat attaccaatc acatttatca
     7441 caatgcaact taatatcggt ttttatcttt taactctcta aacaatcaac agtaattaat
     7501 aattttaaat ctacttttta tgcctttttc gacagttcat aggcagctaa atgatctttt
     7561 aatgcctgag caataggcgg taatggccgg gatgcctcgg atgcctgtcc acttgtcggt
     7621 ggcggcggtg ccgggcggct tggcttaaga cctgaagtat caagactttt cgatttttca
     7681 taggcagcca aatgatcttt tagcgcctga gcaatgggcg gtaaagaccg ggatgaccca
     7741 gataccaacc cgcttgtcgg cggcggcggt gccgggcggc ttggcttaag gcttgaagta
     7801 tcaagacttt tcgacttttc ataggcagct aaatgatctt ttaatgcctg agcaatgggc
     7861 ggtaaagggc gggatgcccc ggaagcctgt ccacttgtcg gcggcggtgg ggccggacga
     7921 gagggagtaa atgaagtcgc cgggctactc acattaaaag tcgttctcgc attgatattc
     7981 gacgtaccgg gagaaaaagg agattgagcc ctaatcggat tcagacgaac agcctgaggg
     8041 gacactgaaa agccagataa ccccgcagag cttacccggc ttaccgcact agtaatatgc
     8101 cgtcctagtg tagaaacagc ttgactaatt ccatta
//
LOCUS       DAZGOB010000177         8026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGOB010000177 DAZGOB010000000
VERSION     DAZGOB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.177"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            214..738
                     /locus_tag="ACHLIF_004570"
     CDS             214..738
                     /locus_tag="ACHLIF_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFO9608432.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            822..1508
                     /gene="lpfB"
                     /locus_tag="ACHLIF_004571"
     CDS             822..1508
                     /gene="lpfB"
                     /locus_tag="ACHLIF_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO9608433.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1532..4063
                     /gene="lpfC"
                     /locus_tag="ACHLIF_004572"
     CDS             1532..4063
                     /gene="lpfC"
                     /locus_tag="ACHLIF_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO9608434.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            4074..5129
                     /gene="lpfD"
                     /locus_tag="ACHLIF_004573"
     CDS             4074..5129
                     /gene="lpfD"
                     /locus_tag="ACHLIF_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO9608435.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            5135..5659
                     /gene="lpfE"
                     /locus_tag="ACHLIF_004574"
     CDS             5135..5659
                     /gene="lpfE"
                     /locus_tag="ACHLIF_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO9608436.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5912..7603
                     /gene="eptB"
                     /locus_tag="ACHLIF_004575"
     CDS             5912..7603
                     /gene="eptB"
                     /locus_tag="ACHLIF_004575"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO9608437.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7695..7771
                     /locus_tag="ACHLIF_004576"
     tRNA            7695..7771
                     /locus_tag="ACHLIF_004576"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7729..7731,aa:Pro,seq:cgg)
     gene            7806..7939
                     /locus_tag="ACHLIF_004577"
     ncRNA           7806..7939
                     /ncRNA_class="other"
                     /locus_tag="ACHLIF_004577"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2105 a         1830 c         2013 g         2078 t
ORIGIN      
        1 gtttatatat catgtaaata taaatatatt cctaaaaaaa tagtcgttgg aagcgttttt
       61 gtgtgcaatt aattgaagtt gaagtacatt tcaatttatt gcaaaaacta atcttcagta
      121 aaaataattt tcttttttag caccatttgt ataatctgcg ccccgttgta tgaacaacat
      181 caagcatatt tatctatata aatggagtta taaatgaaaa aggttctgtt tgccttatct
      241 gcgcttgcgc tgacctctac ttccgtattc gcagctgatg cgggcgacgg tactgttaaa
      301 ttcaccggtg agattgttga cgccgcttgt gttgtgtctc cagacactca aaaacaagaa
      361 gttgttctgg gtcaggtgaa caagtctgta tttactgcta tgggtgataa atccacagca
      421 acgccgttca aaatcaaact ggaaaactgc gatatctcca ctttcaaaaa tgtggaaatc
      481 agctttaacg gcgttggcga tgcagacaac agcaaactgg tttctgtaag cactgaacca
      541 ggtgccgcaa ctggcgtagg tatcggtatt tatgataaca ccaataccct ggttgacctg
      601 aatactggta aatctgctac tgtcctgaaa gaaggtcaga ccgttctgta tttcaccgct
      661 aactatgtct ctaccaaaga tgcagtaacc acgggttacg gtaacgctga agtcgacttc
      721 aacctgactt acaactaatc gatcctgacg gaacgatcat ctctcaatgg caacagagac
      781 tgtgttgcca ttattcacac caatgactca gggaccgaat catgaaacgt attagctttg
      841 ctgtgctggc acttttaatg accgcacaac agtgttttgc tggtggcgta gccttaagca
      901 cgacgcgtgt tatttatgat ggcggcaaga aagaagcgtc gctgaccgtt gaaaaccata
      961 ataaaaatga agagtttttg atccagtcat ggattgatga tgtgaatggc aataaaaaaa
     1021 caccgtttat tattacaccg ccattattca aacttgatcc tgataaaaat aacgtcttac
     1081 gtatcgtcaa tattgaagat accttaccta aagatcgtga atccgtttac tggattaatg
     1141 taaaggccat tcctgcgaaa agtgctgaaa atgaaaataa aaacgtctta cagatagccg
     1201 ttcgcacccg tttgaagttg ttctatcgtc caaaaggttt gccgggaagc aacctcgagg
     1261 ccatgaaaca gttacgtttc cagcgtcaaa ataaccaaat tgtggttaat aacgactccg
     1321 cttataacct gacctttaat cagttttcca ttaatggaca aaagattgaa cgggcgggaa
     1381 tggttctcgc gaaaggaaag ttaaacatta accttccagc cggtacaggc aacgcgcacg
     1441 aagtgaaata cagcattatt aatgatttcg gtgttgctgg cgaaatgctg acgaagccga
     1501 ttaactgatc cttttacaag gcgcttttac tatgcattgg atgcatttac ctctctatca
     1561 ctacagagcg catttttcct tctctctgct ggcattaacg attgccagct cgttacctgc
     1621 gtatggcgga aaatttaacc ctaaatttct cgaaaacgtt cagggtatcg atcaacacat
     1681 cgacttgtcg gtatacgact cacctgtcgg ccagcaaatc cccggtaaat accgggtttc
     1741 ggtgtttgta aacgaagaaa aaatggcttc ccggacgctg gattttagta ccgccagcga
     1801 agcgaaacgc aaagccagcg gtgagtcgct tatgccttgc cttagccggg tgcaactgga
     1861 agaaatgggc gtgcgcgttg acagctttcc ggcgctgaag atgtcaccac cagaagcctg
     1921 cgtagcgttt gatgaaatca ttccccaggc caccagccgc tttgatttta atactcagac
     1981 gctgcatctt tcctttccac aggcggcaat gatgatgacc gctcgcggca cggttgatcc
     2041 ttctcgctgg gacgaaggaa tacctgcgct gttgctggat tacagcttta gtggcagcaa
     2101 tggacgcaat gaaggtacag gttcatcttc agattcgacc agtgacagtt attacctgaa
     2161 cctgcgcagc ggtttgaacg ttggtccgtg gcgtttacgt aataacagta tctggaaccg
     2221 taccgatggc aaaaaccaat gggataacgt tggtacatcg ttaaaccggg caattattcc
     2281 acttaaatca cagataactc tgggtgatac cgccacgcct ggcgagattt ttgatagcgt
     2341 acaaatgcgt ggggcgttac tggcatccga cgatgaaatg ttgccggaca gccagcgtgg
     2401 ctttgctccg gtagtgcgcg gcatcgccaa aagcaacgct gaagtgagta tcgaacagaa
     2461 cggctatgtc atttatcgca cttttgttca gccgggtgca tttgagatta acgatctcta
     2521 cgcaacctcc ggcagcggcg acctgacggt gatcattaaa gaagccgacg gaagtgaaca
     2581 acgttttatc cagccgtttt cggcggtggc gattttccag cgtgaagggt atctgaaata
     2641 cagtctggct gccggtgaat atcgtgctgg taattacgac agcgataaac cacatttcgg
     2701 gcagtttacc gccatgtacg gcttgccgtg gggtatgacc gcctacggtg gtgcattgtt
     2761 gtcggcagat tacaacgctt tagctctggg cctggggaaa aacttcggta ccattggcgc
     2821 agtatcggtg gatgtcactc aggcgaaaag tcagttacgc aacaatgaaa aagaggaagg
     2881 ccagtcttat cgcttcctct attcgaaaag ttttgagggt ggcactgacc tgcgtctgtt
     2941 gggctacaaa tactcaacca gcggctacta caccttccag gaagcaactg atgttcgcag
     3001 tgacgccgac agtgattacc gccgttatca caagcgcagc cagatacagg gcaatattac
     3061 ccagcaactg ggagactacg gctcggttta tttcaatatg acgcagcagg attactggaa
     3121 cgttgacggt aaggaaaact ccttaagcgc cggttatcac ggtcacattg gtcgggtgaa
     3181 ttacagcgtg gcctatacct ggacgagaag tccggaatgg gaagaagacg atcgcttgtg
     3241 gtcattcagt gtttccatcc ctctgggggg cgcgtggagc agttatcgca tgactaccga
     3301 ccagaatggc aagacgtcgc aacaggcctc tgttagcggc acgttactgg aagatcgtaa
     3361 cctgagctat aacgttcagc agggttacac cagtaatggc gtaggctaca gtggcagcgt
     3421 caacatgggg tatatgggtg gctctggtaa cattgacgta ggctataact attctaaaga
     3481 taaccaacag gttaactatg gcgtgcgcgg cggtgtcatc gttcacagtg aaggtataac
     3541 gctttcgcag cctttaggcg aaagtctggc gattgtttct gcaccgggcg cgcgtggtgg
     3601 acatgtggtt aacagcagcg gcgtagaagt tgactggatg ggcaacgccg tggtgcctta
     3661 tctcacacca tatcgtgaaa ccattgtgga attgcgtagc gacacgttgg ggcaaaacgt
     3721 tgaactacag gaagcgttcc agaaagtcgt gcctactcgt ggtgcagttg tgcgttcccg
     3781 ctttgatacc cgcgtcggtt accgtgtgct gatgagctta aaacaggcga atggcaacgc
     3841 agtaccgttt ggtgcaaccg cagcgttaat tgatgagagc aaacctgcca gcagcattgt
     3901 gggtgaagaa ggacagcttt atatcagcgg gatgccagaa gagggcgaat tgcaggtgag
     3961 ctgggggaac gagcaggcgc aacgttgccg tgtgccattc cgcttgccag agaacaagga
     4021 caatgcggct atcgtgatgg ttaatgcggt atgcgagaaa taaggatatt tagatgaaaa
     4081 ttaaaaattt gttggcaggt atggtgctgt tagggacgag cacttttgct ggaaatatat
     4141 gggctgcgga ttggggacca tgtcagacat caaatggggt ggcccatgag tatagttttg
     4201 atttcgtaca gactatccag gtaccttcag aaaataaggc tgggaaaata ttaactcagc
     4261 cctttgcttt aggtacaaaa tattccgctt attgtgaatg cccggatccc attccagata
     4321 atggagttgt gacttatttt aaaggtgtaa cgcttctgcc tgagcctgga actgtagatg
     4381 ggtactataa attcaataat tacattgata ttctcaccaa aatatatgtc tacacacaat
     4441 tagatatccc ggtgcctttt accgatcgaa gcaatggcac ggcacagaaa gaatgtacgc
     4501 cctatactgc taacaattgg gggacaggtg gtaaaggaag tatcagtatt tatatttcac
     4561 atccttttgt tgggcaaatg attattccta aaacacgagt tgcgtccctc tttgggacaa
     4621 aaaagaaagg tgtttacaat gatagtcagc ccatggccaa tgtcagtatt tccgggagta
     4681 ttacagttac tcaaggctgt gaacttgccg ctggcaccgt actggatatt ccattcggag
     4741 aatatcaggc ccatgatttt aaagggcgtg ccggacaacc accacaaaat gttcagaagg
     4801 tacaaaaaga attaagcttt gactgcaata atatttcaga tggcgtcaaa atttatctca
     4861 gtattgatgc aacaccaaat actgcctatc ccagcgcaat tgatttaggc aacgccgatg
     4921 ttggtgcagt cattgaggac ggtaaaggta atattcttaa gccgaatgac agtaatagct
     4981 tattagaaat gaacccaggc tcgctgtatg aagatgtcaa acgcaaagcg acaaccacaa
     5041 ttaccgctta tcccgttagc actaccggaa aattacccgc tgccggggat tatagcggta
     5101 ttgctaccat acatgtcgaa ctggagtaac agtcatgaag cgaacaatac ttgcgttatg
     5161 cctgctgcct ctttcctttt cgtcattcgc ggcaaccacg gatttagggc cgaaaggaac
     5221 attgaaattc acccttaaaa tcagccaggg tgcttgcgaa ctggtgaaag acagcgttga
     5281 ggttgacatg ggcaccgcgg ttctcaaaaa gccggtaaga atcggaacgg aaattaatcc
     5341 cactcctttt tccatcggcc tgaaaaactg tagcgaagtg gtcagagcct acgtgactat
     5401 ggacggtaca cccaatgcag acgactcaaa tctttttgcg ctggatgcgg gtggcgctac
     5461 aggcgttggg ctgaaaatca aaaccgctgc tggagtacaa cagttcccgg aaaatacaac
     5521 gccgacaccg gttgagtttg caattaacgg tgatggcgca catcaactga actatatcgc
     5581 cagctacgtg cctattcgcg ccgatgcaac tattggtcgc gcagatgcaa cggtcgactt
     5641 tactgtacag tacgaataat ttcacatctt aagggccagt taactggccc tttttactgc
     5701 tttttgtaac gctaaagtct cttttcaaac ttgcattttt gtaaatttgt gcttcatgca
     5761 cactctttcc ccacactttt tcgctttgct gtggtctact tattcgcgcg tgtagatttt
     5821 acttatctga ctacctccgc acttttcccc tgccgggcct gaaaagccgc taagcagggt
     5881 gttatcacct gtttgtccag ggtttgtttg catgagatac atcaaatcga ttacacagca
     5941 gaagctgagc tttttgcttg caatctatat tggccttttt atgaatggcg cggtttttta
     6001 tcgccgcttc ggcagctatg cgcacgattt taccgtctgg aaaggcgttt ctgctgttgt
     6061 tgaactggcc gccaccgtac tggtgacctt ctttttacta cgtcttctat cgctgtttgg
     6121 ccgccgcagc tggcgtattc tggcgtcgct ggtggtgctc ttttccgcag gtgccagcta
     6181 ttacatgacc ttccttaatg tggtcattgg ttatggcatc atcgcttccg tcatgaccac
     6241 cgatatcgac ctgtcaaaag aagttgttgg tctgaacttt attctctggt taatcgccgt
     6301 tagtgcattg cctcttatcc ttatctggaa taaccgctgt cgctacacct tgctccgaca
     6361 actgcgaacc ccagggcagc gtattcgcag cctggcggtc gtcgtactgg cgggtattat
     6421 ggtttgggca ccgattcgtt tgctggatat ccagcagaag aaagtggaga gggcgaccgg
     6481 cgttgatttg ccgagttatg gcggtgtcgt agcgaactct tatctgccat caaactggct
     6541 ttctgcgttg gggctgtatg cctgggcgcg ggtcgatgaa tcttccgata ataattcatt
     6601 gcttaatccg gcgaagaaat tcacttatca ggcaccgcaa aacgttgatg acacttatgt
     6661 cgtgtttatc atcggtgaaa ccacgcgttg ggaccatatg ggcattttcg gctatgagcg
     6721 taataccacg ccgaaactgg cccaggagaa aaatctggcg gcgttccgtg gttactcctg
     6781 tgataccgca accaaactct cactgcgttg catgtttgta cgtcaggggg gcgcggaaga
     6841 taatccgcag cgcacattaa aagaacagaa cattttcgcg gttctgaagc agttaggatt
     6901 cagttctgac ctctacgcta tgcagagcga aatgtggttc tacagcaaca cgatggcgga
     6961 caacattgct tatcgtgagc agattggtgc ggagccacgt aatcgtggca agccggtaga
     7021 tgatatgttg ctggtagacg aaatgcagca atcgctaggg cgcaacccgg atggtaagca
     7081 tctgatcatt ctgcatacca aaggttcgca ttttaactac acccagcgtt atccgcgtag
     7141 cttcgcgcag tggaagccgg aatgtattgg tgttgatagc ggctgtacca aagcgcagat
     7201 gatcaactcc tatgacaact cggtgaccta tgtggatcac tttatctcca gcgtgattga
     7261 tcaggttcgc gataagaaag cgattgtgtt ctacgcagct gaccacggtg agtcaattaa
     7321 tgaacgcgag cacctgcacg gcacgccgcg tgaactggca ccgccggagc agttccgcgt
     7381 accgatgatg gtctggatgt cagataaata tctggaaaat ccggccaatg ctcaggcatt
     7441 tgcgcagctg aaaaaagaag ccgacatgaa agtgccacgc cgtcatgtag agctgtacga
     7501 caccatcatg ggttgtcttg gctatacttc accggatggt ggaattaacg aaaacaacaa
     7561 ctggtgtcac atcccgcaga caaaagtggc agcggctaac taagcggcct gctgactttc
     7621 tcgccgatca aaaggcattt tgctattaag ggattgacga gggcgtatct gcgcagtaag
     7681 atgcgccccg cattcggtga ttggcgcagc ctggtagcgc acttcgttcg ggacgaaggg
     7741 gtcggaggtt cgaatcctct atcaccgacc aaattcgaaa agcctgctca gcgagcaggc
     7801 ttttttgcat ttgaagccca tgaggatgag aacctccggg ggcggggagg ttcgactcga
     7861 gcgaagcgag agaacgttgc gccagcaacg gcccgcaggg cgagccacga agtggcgagt
     7921 aatcctctat caccgaccaa attcgaaaag cctgctcaac gagcaggctt ttttgcattt
     7981 gtagctcatg aggatgagaa cctccggggg cggggaggtt cgactc
//
LOCUS       DAZGOB010000178         8018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGOB010000178 DAZGOB010000000
VERSION     DAZGOB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.178"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(203..1396)
                     /gene="tyrB"
                     /locus_tag="ACHLIF_004578"
     CDS             complement(203..1396)
                     /gene="tyrB"
                     /locus_tag="ACHLIF_004578"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO9608438.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1631..2728)
                     /gene="alr"
                     /locus_tag="ACHLIF_004579"
     CDS             complement(1631..2728)
                     /gene="alr"
                     /locus_tag="ACHLIF_004579"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO9608439.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVDLSFCNI"
     gene            complement(2781..4196)
                     /gene="dnaB"
                     /locus_tag="ACHLIF_004580"
     CDS             complement(2781..4196)
                     /gene="dnaB"
                     /locus_tag="ACHLIF_004580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO9608440.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4279..5262
                     /gene="qorA"
                     /locus_tag="ACHLIF_004581"
     CDS             4279..5262
                     /gene="qorA"
                     /locus_tag="ACHLIF_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO9608441.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5428..5670)
                     /gene="pspG"
                     /locus_tag="ACHLIF_004582"
     CDS             complement(5428..5670)
                     /gene="pspG"
                     /locus_tag="ACHLIF_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO9608442.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5804..6841)
                     /gene="dusA"
                     /locus_tag="ACHLIF_004583"
     CDS             complement(5804..6841)
                     /gene="dusA"
                     /locus_tag="ACHLIF_004583"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO9608443.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNTQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7204..7911)
                     /gene="yjbM"
                     /locus_tag="ACHLIF_004584"
     CDS             complement(7204..7911)
                     /gene="yjbM"
                     /locus_tag="ACHLIF_004584"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO9608444.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNLGDNK
                     DAMPIEQCTFDVENKNDLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(7935..>8018)
                     /gene="yjbL"
                     /locus_tag="ACHLIF_004585"
     CDS             complement(7935..>8018)
                     /gene="yjbL"
                     /locus_tag="ACHLIF_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO9608445.1"
                     /translation="DIIIAKIDLFIQRITHNLWHARKKMCF"
BASE COUNT         2089 a         2070 c         1893 g         1966 t
ORIGIN      
        1 tgcagaaatt attactccac ccaaaaaaac aggataagga attccagata attgggataa
       61 gcttaacaat aaaagaacta acaacgttta ttatctctca cgtagaacga tggcatcaaa
      121 aaaatcatct ataaagaaag agtgaaagcg acgacagttt aatttcactg caggctgggt
      181 agctccagcc tgctttcctg cattacatca ccgcagcaaa cgcctttgcc acacgttgta
      241 catttgccgt atttaacccg gcgacacaca tgcgaccgct ggcgatgaga tagacaccaa
      301 attcttcacg tagtcggtca acctgagcgg cacttaaacc ggtataactg aacatgccgc
      361 gctgattaag cagataatcg aaattgcgtt ctggcatctc tgtgcttaat accttcacca
      421 gttcctgacg cattgccaga atgcgagtac gcatctcttc tacttccgcc agccagctgg
      481 ctttcaatgc ctcgtcattc agcactgcag ccaccacctg cgcaccaaaa ttcggcgggc
      541 tggagtagtt gcggcgaact gttgctttca attgccccag tacgcggcca gcggcttcgg
      601 catcttcaca cagaacagaa agtccgccga cgcgctcgcc gtaaagggag aaaattttcg
      661 agaacgaatt gctcaccaga gcgggtaatc cagcgctggc aatggcgcga atagcgtagg
      721 catcctcttc cataccggca ccaaatcctt gataggcaat atcgaggaat ggaataagct
      781 cgcgggattt gagaatttca atcaccgcat cccactgatc attagtgaga tcggcacccg
      841 ttgggttgtg gcaacatgga tgcagcaaca caatactgcg ggcaggtaat gttttcagcg
      901 tcgccaacag gtcattaaag cgcacgccgt tagtcgcttc gtcataccag gggtaagtac
      961 tcacttcgaa tccagccccg gcgaatattg ctacgtggtt ttcccaggta ggatcgctga
     1021 cccagacgcc tgattccggg aagtagcgtt tcaggaaatc cgcgcccact ttcaatgccc
     1081 ctgagccgcc aagggtttga atggttgcta cgcgctgttg ttgcagtact ggatggtccg
     1141 caccaaacag cagcggcgca atggcatggc gatagctgtt aagcccttcc atcggtaaat
     1201 aaagcgaagc gccatgaggc tgcgcattca ggcgcgcttc cgcctccgcc acggcttgca
     1261 gttgtggaat aattccgtct tcgttgtagt acagaccgat acttaaattc actttgtcgc
     1321 tgcgagggtc ttctttaaaa cgctccataa gcgtaagaat cgggtcgcca gcgtaggcgt
     1381 caactttttg aaacacgcga tggttctcca ggtttacggg caggtggtta aaacacaata
     1441 aaccggaaga aggcgaagat cgagtggatg ttcaggagcg aacggcaatt agcaacagag
     1501 tgagactcat gacaaacgta catccgccag aggcacgacc tttataagag catgaaagaa
     1561 catcaactta ttgaattttt aggattttat tggccggata aggcattcac gccgcatccg
     1621 gcacagacaa tcaaatatta cagaacgata aatccacgta tttcatcgcg acccttgaag
     1681 tcaggcgcgt aataagttcg taagcgctta ctttcgtcat ttcagcgata cgttctacgg
     1741 gcaaaccttc gccccataaa atgaccggat ccccggcttt gtcctgcgct tgtggaccta
     1801 agtctacgca gatcatatcc atcgccacgc gcccgacaat cggtacttcg cgaccgttca
     1861 ccagcactgg cgtaccggac ggcgcggcgc gcggataacc atcgccatag cccatcgcga
     1921 ctacgccaag acgggtatca cgttcgctta cccaggttcc accataacca acaggttctc
     1981 cggctttatg ctcacgcacg gcaatcaggc tggaggttag tgacatcact ggctgacagc
     2041 caaaatcggc accggtggag cgatcttcca gcggcgagac gccataaaga atgatgcccg
     2101 ggcgcaccca gtcaaaatgc gactgtggcc acagcagaat gccacccgat gcggcaatgg
     2161 aacgttgacc aggtttgcct tcgcaaaagg tattaaagat agcgagttgt ttctcggttg
     2221 cgccacattt tggttcatcc gcgcgcgcaa aatggctgac gatattcacc ggctgacgaa
     2281 cgtttttgca ctgggtcagg cgatgataaa acgcctcagc ctgttccggc cttacgccca
     2341 gacggtgcat accggtatcg agtttcatcc agacggtaac cggctcgtcc aggctagcct
     2401 cttccagcgc agccagctgt tcttcgttat gcacggcggt atgaaaatgt tgcgcagaaa
     2461 tcgtcggcag atctctggca tcaaaaaagc cttcgagtaa cagtacaggt ttggtgattc
     2521 cccccgcacg cagtcgcaga gcttcttcga gacgggctac gccaaaggcg tcagcatcgg
     2581 ggagcgttcg cgcggtctca agaagaccgt gaccataagc gttcgctttc accaccgcaa
     2641 ccattttact ggcaggggcc agttcacgaa gacgttgcag gttgtgtcgc agagcgcggc
     2701 ggttaatcac aacagttgcc gcttgcattt gtgttccttg ataagtgttt gctttaatta
     2761 cctaattcat aaaataatta ttattcgtcg tcgtactgcg gccccgcata gttgtcgaag
     2821 cgcgaccatt gaccgttaaa ggtcaggcgt accgtcccga ttgggccgtt acgttgttta
     2881 ccgataataa tttccgcgat gccttttaaa tcactgtttt cgtgatacac ctcatcacga
     2941 tagataaaca tgatcaagtc cgcatcctgc tcgatagagc cagattcacg caggtcggag
     3001 ttgaccgggc gtttgtcggc acgttgttcc agagaacggt tcaactggga cagcgccacc
     3061 accggcacgt tcagttcttt cgccagtgct ttcagcgagc gggagatttc tgcaatttcc
     3121 agcgtacggt tatcggaaag cgccggtacg cgcatcagtt gcaggtagtc gatcatgata
     3181 agcccgatgc cgccgtgttc acgggcaata cggcgtgcgc gggaacgcac ttccgttggc
     3241 gtcaggccgg aggagtcatc gatatagata ttgcgttttt cgagcaaaat acccatggtg
     3301 ccggaaatgc gcgcccagtc ttcatcatcg agctgcccgg tacggatttt agtctggtca
     3361 acgcgcgaca gcgacgccag agaacgcatc ataatctgtt ctgaaggcat ctccagcgag
     3421 aagataagta ctggtttatc ctgcaacatc gccgcgtttt cgacgaggtt catcgcaaat
     3481 gttgttttac ccatcgacgg acgcgcggcg acgatgatca aatccgacgg ctgcaagcca
     3541 gcggtttttt tgttgagatc gtcataaccg gtgtttactc cggtaacgcc atcgtgtggc
     3601 tgctgaaaca actgctcaat acgcgccacg gttgcgtcga gcacatcggc gatgttcttc
     3661 ggcccttcgt ctttgtttgc acgactttcg gcaattttaa agacgcggga ttcagcaagg
     3721 tccagcagat cttcgctggt acgcccctgc ggatcaaaac cggcttcagc aatctcattc
     3781 gcaaccgaga tcatctcacg gacaacggca cgttcacgca cgatgtcagc ataagcactg
     3841 atgttcgccg cacttggcgt attttttgac agctctgcca gataagcaaa accaccgacg
     3901 ctatcgagtt gcccctggcg ttccagcgat tccgcaaggg taatcagatc gataggacta
     3961 ccgctttcct gcaaacgcgc catttcagta aagatatggc ggtgtgggcg ggtgtaaaaa
     4021 tcgtctgcca ccacacgctc ggctacatcg tcccagcgtt cgttatccag cattaaaccg
     4081 cccaacaccg actgctccgc ttcgatcgag tgcggaggca ctttcagccc ggcaacttgt
     4141 ggatcgcgtt cgcggggttc agcctgctgt ttgttgaagg gtttatttcc tgccatagtg
     4201 aatggagtta ccgagataaa gaatgggtcg aaactttacc atatgaagca gcccctgacg
     4261 atacgttctg gaggacacat ggcaacacga attgaatttc acaagcacgg tggcccggaa
     4321 gtacttcaag ccgtagagtt cactcctgcc gatccggcag agaatgaaat ccaggtcgaa
     4381 aataaagcca tcggcatcaa ttttatcgac acgtatatcc gcagcggcct ttacccgccg
     4441 ccatcactac ccagcggatt aggtaccgaa gcggcaggca tcgtgagtaa agtcggcagt
     4501 ggtgtaaagc atattaaggc aggcgatcgt gttgtctatg cgcagtcggc gttaggcgct
     4561 tacagctctg tgcataacat taatgcggat aaagcggcga ttctgcctgc ggcaatttct
     4621 tttgagcaag ctgcggcatc cttcctgaaa ggcttaacgg tttattatct gctgcgtaaa
     4681 acctatgaaa ttaaacccga tgaacagttc ctgttccacg cagcggctgg cggcgttggc
     4741 ttgattgcct gccagtgggc aaaagccctg ggcgcgaaac ttatcggcac cgtaggaacc
     4801 gcgcaaaaag cgcagagcgc gctaaaagcg ggcgcgtggc aggtaattaa ctatcgtgaa
     4861 gagaatctgg tcgagcggtt aaaagagatc accggcggta agaaagtgcg cgtggtgtac
     4921 gattccgtgg gcagagacac ctgggaacgg tcgctggatt gcctgcaacg ccgcggctta
     4981 atggtcagtt ttggcaactc atcaggtgcg gttaccggtg tgaacttagg cattctcaat
     5041 caaaaaggct cgttgtatgt gacacgccct tccctgcaag gctatatcac cacgcgggag
     5101 gaattaaccg aggccagtaa tgaactgttc tctttgattg ccagcggtgt gattaaggtc
     5161 gatgtcgccg agcagcagaa atatccgctg aaggatgcgc agcgtgcgca tgagattctg
     5221 gaaagccggg cgacgcaagg ttccagcctg ctgattccat aaaagaaata gggcttccac
     5281 ctgggaagcc ctttcttttt atagttcggc tgtatgtagg gtacagcacg atgaatctgt
     5341 tagaggcgca atagtgacag atttgattat caattcctat tttgttctaa ggataaaacc
     5401 ttaggttgtg atcatccgca caatccctta gtaacgccag cggtcataac gctgatattt
     5461 aggcactttt ggtgctttaa tcgccttaat aacccacacc accgcaatcg ccagtagtaa
     5521 ccacggcagc aacttaatca tcaatgccag cataccgccg aggaacataa tggccgtcgc
     5581 cacaaccagc gcggcgataa tgcccagcaa cgaaacgccg gtgaccatca gcatgacaaa
     5641 aaagccaatc acaaaaagta gttccagcat gatgctctcc caaatatgaa atctcttgct
     5701 ggcattacaa gaatcatgcc aaaaataatc tattgattta acagcaaaac gccccgcgac
     5761 ggtgcgcagg gcgtggtgaa tttgactact ttttggtgaa aagttaacgc ttatccgcca
     5821 ccagtttgag cgcgtgttcc agcacattaa tgtctgcacc cgctttatgg gcattttcac
     5881 ttaaataacg ccgccactgc cgcgcgccag gaataccctg gaacaagccc aacatatgcc
     5941 gggtaatatg gccgagatac gtcccctggc tgagttcacg ctcaatgtac ggatacatgg
     6001 cgcgcactac cgccaccgga tcggcatcgg tatccgagga accaaagatc tcccggtcta
     6061 ccgccgccag aatacccgga ttctgatacg cctcgcgccc gaccatcacg ccatccatat
     6121 gttgcaggtg tgctttggcc tcttccagcg acttgatacc accgttaatc gacattgtca
     6181 gatgcggaaa gtcacgcttc agttgataca cacgcggata atcgagcggc gggatttcac
     6241 ggttttcttt cggacttaac cccgaaagcc aggctttacg tgcgtggatg ataaacatct
     6301 cacactcgcc tttgccggaa acggtgttga tgaaatcgca gagaaattca tagctgtcct
     6361 ggtcatcgat gccaatacgc gttttcaccg tcactggaat cgacaccaca tcgcgcatcg
     6421 ctttcacgca gtcggcaacc agctgcgtat tacccatcag acacgcacca aacatgccgt
     6481 tctgcacccg gtcagacggg cagccgacat tcaggttgat ctcatcatat ccacgcgctt
     6541 ctgccagctt cgcacactgt gccagcgccg ccggatcgct accgccgagt tgcaacgcta
     6601 ccggatgttc ttcttcactg tacgccaggt aatcaccttt accgtgaata atcgcccctg
     6661 tggtcaccat ttcggtatac agcaacgtat tgcgggaaag cagacgcaag aaatagcggc
     6721 aatgtctgtc cgtccagtcg agcattggtg caacgctaaa ccgaccactc cagtgaacgt
     6781 cagttttttc aggcattgcg ctggtttggt tgattttttg catttcagaa ttaccgtgca
     6841 ttttcaaatg tagagattat tttatcgata tatcatgggg ttatgttatt cagcatcact
     6901 attcaggagg ctcaatagcg gggtactata ccataacaac aggaagcgcc tgtctcattg
     6961 caaaagaaaa ttgagatcct ctcaaggcat gaagctctca cgaagtgatg gaaataatct
     7021 tattagccgt taactttgtt aaggccaatg ataaacaatc caggttcgac gataaataaa
     7081 aaatcacaca ttaaactctg gtgatatatc tccctgctaa tagcattgat agagaaaaaa
     7141 gaacccaata aatattgggt ccttttatat aatgccttcc atactatcga agaacttcac
     7201 atattatttc tccgatttaa ccccgaacaa atcataaatt aatttagaag tgtctgtaag
     7261 tattttaatc tcttcctttg aggttgggtc aaacgacttc gcgaagctaa ttaattgtgg
     7321 tgcagcatct cgcattttgc ttaaaatatc aggatcgagc gttccttcat tcaccagatg
     7381 tgacatctta tccagatagc catcaaaatt catttctccc ccatcagcca gacgcttcat
     7441 ctctcccagg tacttcttca tatcacgttg agataatttt tcaaactgag ctttcaggta
     7501 gctctcatta ttttcattaa tatacactgt ttctgataaa tctgcgtagg cactagaata
     7561 tgaaagtgaa attaacagcg aagcaaataa ttttaagctg ttgtcatcat gttcctggca
     7621 ggcattaaca aacgttaaaa aaccagaacc tattgcttga aaatgtatat tcgctaaagg
     7681 ttcattatcc ttagattcct cataaaatag ctgaaccgtg gagggaaggt cattcttatt
     7741 ttctacatca aaagtacatt gttcaatggg cattgcatct ttattatccc caagattaca
     7801 tatactatta atcgcgaaat gaaaaatagc ccggtcaaca actgatgcaa taaagtcacg
     7861 atcgtttaaa tcctcatcag tgcaatcgtc aatatactta ttgacccaca ttttgagatc
     7921 atttatttgc tcaattaaaa acacattttt ttacgtgcat gccataaatt atgcgtgatt
     7981 cgttgaataa acaaatctat ttttgcaata attatatc
//
LOCUS       DAZGOB010000179         7942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGOB010000179 DAZGOB010000000
VERSION     DAZGOB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.179"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..740)
                     /locus_tag="ACHLIF_004586"
     CDS             complement(<1..740)
                     /locus_tag="ACHLIF_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFO9608446.1"
                     /translation="MNKKLYLFPDSNIFLQCKDLTQVNFSEITACDEVCIIITRPIQQ
                     EIDRQKGQGNSRLSKKARKAASLFNQVVDSTDMTLVIRERSPRVFLTMDLSLKPCEQL
                     SEQLDYQEADDRFVGIAAGYCADDPDSEVAIITNDSGPRFSAIKHNIICYKVPSSWLL
                     PAEPDEKDKQIKLLEAKLKQFTQSMPDFSIKVADDEQLNIVLPYYTALSEYEVDNLLC
                     QLTDAFPLVTNFDNEQTTPQSLEKIIKS"
     gene            complement(966..1766)
                     /locus_tag="ACHLIF_004587"
     CDS             complement(966..1766)
                     /locus_tag="ACHLIF_004587"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO9608447.1"
                     /translation="MTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQI
                     SEMLMDIEIDHADQTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLP
                     SFAITRSSGDRHQFRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPSKPKKEKSSSR
                     EGDYMEGAEGNVFVLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLAHGRAELYPEM
                     HGTVTGFKSEIDNQDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            1727..1819
                     /locus_tag="ACHLIF_004588"
                     /pseudo
     CDS             1727..1819
                     /locus_tag="ACHLIF_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2233..3417
                     /locus_tag="ACHLIF_004589"
     CDS             2233..3417
                     /locus_tag="ACHLIF_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO9608448.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGASCWYDVVDNGTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            3417..3929
                     /locus_tag="ACHLIF_004590"
     CDS             3417..3929
                     /locus_tag="ACHLIF_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO9608449.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            3984..4349
                     /locus_tag="ACHLIF_004591"
     CDS             3984..4349
                     /locus_tag="ACHLIF_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9608450.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            4358..4513
                     /locus_tag="ACHLIF_004592"
     CDS             4358..4513
                     /locus_tag="ACHLIF_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO9608451.1"
                     /translation="MCFEYIEDFVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            4500..7307
                     /locus_tag="ACHLIF_004593"
     CDS             4500..7307
                     /locus_tag="ACHLIF_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9608452.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGILKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVAVSDA
                     NGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLGSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SRVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRGLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAEKARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            7320..7808
                     /locus_tag="ACHLIF_004594"
     CDS             7320..7808
                     /locus_tag="ACHLIF_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9608453.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSATSSVKG
                     LL"
BASE COUNT         1848 a         1933 c         2237 g         1924 t
ORIGIN      
        1 aatgacttga tgattttttc tagtgattgt ggggtagttt gttcattgtc aaagtttgta
       61 accaatggga atgcatccgt tagttgacaa agcaaattat caacttcata ttcagataaa
      121 gctgtataat aaggaagtac aatatttaat tgctcgtcat cggctacttt aatgctgaaa
      181 tcgggcatag actgagtgaa ttgcttcagc ttagcttcca gcaatttaat ttgtttatcc
      241 ttttcatctg gctcagcagg aagcaaccat gaagaaggca ctttatagca gattatatta
      301 tgttttattg ctgaaaaacg tggaccgcta tcattggtaa tgattgccac ctccgaatct
      361 ggatcgtcag cgcaataccc tgccgcaata ccaacaaaac gatcgtcggc ctcctgataa
      421 tccaattgtt cagataactg ttcacatggt ttcaggctta gatccatggt aagaaaaact
      481 cgcggcgagc gttcacgaat aacaagagtc atgtcagttg aatcaactac ttgattaaat
      541 aggctagcag ccttccttgc ttttttggat aggcgggaat taccttgccc tttctggcgg
      601 tcaatctctt gctgaatggg ccttgtaata atgatacata cttcatcaca agcagtaatc
      661 tcggaaaaat ttacctgcgt caagtccttg cattgtagaa aaatgtttga gtctgggaat
      721 aaatacagct ttttgttcat tcaaacctca actatgtcaa tggtgttaga ttaatataat
      781 aatagttatc aattggttat caaagcatga ttcttaccac ccctgatgtc aggtaggaaa
      841 acaccatatg tttctgctct gtttttcctt agaaataggc atccttgctg gaacaaagcg
      901 agcagctata ttgactctat gttgtgagtg tgttgatccg ttctgagtta atacatttaa
      961 gttgctcact ctgtttctgc tatccattcc gggatttttg cctcaagctc aagctgcgtg
     1021 gtaaagccgc tgttatcaat ggtgtgctcg gctttcgcaa taatccagtc ctgattatca
     1081 atctcgcttt taaatcctgt taccgtgcca tgcatttcgg ggtagagttc tgcacgtcca
     1141 tgcgccagtg tgatggagaa tgatgcggct ccgcgttgta gctgctgcca ctttgccgcc
     1201 gctgcacgtc ttgctgcctg ctcgttctga taagttttgc gtaacacaaa cacgttgcct
     1261 tccgcacctt ccatatagtc gccttcccgg ctgctgcttt tctccttttt tggttttgac
     1321 ggtttgcggc gtttcacgct gacttttttc tttttcccgt aattaagatc aagccagtaa
     1381 gcgcgtaccc ccgtatacgc ttcgcggtca gcaatgcgga actgatggcg atcgccgctg
     1441 ctgcgtgtga tggcgaacga gggcaacggc tggccctgtg cgttcacgcc accaccgggc
     1501 atgatgaata acagattacc gctttttacc gtggtgattg cgcccagcat ttccgccatg
     1561 cgcgtaagga aggacatgtc gctttcttcg gtctggtcgg cgtggtcgat ttcgatatcc
     1621 atcagcattt cgctgatttg cggtttcaga ccataccgat gagcgatggc ggataccaca
     1681 cgctcaacgg tcacatcatg ccaggacacc tcacgtttaa cgttaaattc atcccgaaaa
     1741 tctgcgcttc tggctgaaac agtcagcctg tccggcggtc cttcgtgagc gatttcatca
     1801 acaatgtaag tgcctttttc tgtcagcggt tcccctttcc agccaatgag aaccgtcaga
     1861 cgcgcgcccc gtggcggtag ctgcaactgg ccataggcat catccagcgt gatggtgagc
     1921 tggtccgcct caaatccccg gttgtcggtc agtgacaggc tcatcaggcg ttctgccacg
     1981 ccggacagcg ttttaccctc ggcgagaata tcaaaatccg gcattttcac ggggtctgtg
     2041 ccctgactga gcaattgcat ggtggtgtcg gtcatctgtt ccctccctgt gcggcatggt
     2101 cgcatgtgcg tgcggagggg gttactgctt tttgttgtcg ccgtggcggg agaatagcgc
     2161 aggggtgaga ttacgcgcgt ggtgggtgat gattgttgct gaatcattta acggatacaa
     2221 ggggctgaag ctatgagtga aactcgtttt catggtgccc gtgttacgga aaataccgac
     2281 ctggtaacag cgattaacga tgttgattcc agcgttatcg gtatcgtggc aacggcggat
     2341 gatgcggacg cgaagctgtt cccgctgaac aagcccacat tgctgacccg cgtcaatgac
     2401 gtgctgggaa aatgcggaac aacggggacg ctttatcgtg cgcttaaggc catcgcagac
     2461 caggtgagca caaaggtgat cgtcgttcgc gtggctgaac acaaagaaga agacggaaag
     2521 acgcaggatc aactggttat cggtggttct gaggatgacg gcagctatac ggggatgtat
     2581 gcgctgcttg ttgcagagca ggatgaaagc atcggatacc gtccgcgtat tctggccgcg
     2641 ccggagctgg acacggaggc tgtaacaaaa tccctgtgcg tgattgcagg taaactgcgc
     2701 gcgtttgtgt atgcctcatg tcacggctgt aacacgatgg ctgaggcaat tacctaccgc
     2761 cagaaattca acgaacgtga ggtgatgctc ttatggccgg acttcatcgc ctacaacccg
     2821 aaaagtggca aaaacgaaac gttccccgcg cctgcctatg cgtgcggcct tcgtgcgtac
     2881 attgaccatg agcagggctg gcacaaatcg ctgtccaacg ttccggttaa aaatgtgctg
     2941 gggatgtcca ggcatgtgtt ctggtcgttg caggccgaag acagtgatgc caacagcctc
     3001 aacaacaaag aaatcacgac cattattcgt cgcaacgggt tccgcttctg gggcaaccgc
     3061 acaccggaaa cgaacgccta catctttgag gtgtataccc gaaccgcaca ggtgctggct
     3121 gattcaattg cggaagcgca gtttgaaacc atcgacagtc cactgacgcc tgcgaacgtg
     3181 aaagatgtta tcagtgccat cagggcaaaa ctggattcac tggttactgc cgggaaactg
     3241 attggggcgt cgtgctggta tgacgtggtg gataacggca ccacgaattt acgtcagggg
     3301 cgcgtgcgta ttcgctacaa atatacgcct gttcccccgc tggaagacat ggagctttac
     3361 cagtcgttta ctgatgaatt ctttggtccc gcatttgcgg tgctgggagg tgcctgatgg
     3421 ctgtaccaaa acatcttcgc ttttttacgc tgtttgtgga tggtgaaaac gaagtgggta
     3481 aggtgacgtc cgtcactctg cctaagctga cgcgcaaaac cgacagctac cggggtggtg
     3541 gcatgatggg tgcggtaagt attgatctcg gtctggacga ctccgcgctt gatgcgagct
     3601 ttgtcatggg gggcgcagtt cgtgagctgt tccttaagta tggcggcacg attgacggca
     3661 cgctgctgcg ttttgcgggt gaatactaca ccgatgcaga aagcgacttg tatgaagtcg
     3721 aaatgcgcgg acgtgtgacg gaaattgata tgggggaagc caaacagggc gaagccacat
     3781 cacacactta cgccattaaa aacacctact acaagctgag cgttaacgat cgcccgttgt
     3841 gggagattga cctgctgaac ttcatttacc ggaaggacgg caaggacatt gtgcccgatc
     3901 gcatccgttc cgcgcttggg cttggctgat aagtaatatg caggcggcgc agtgcgtcgc
     3961 ctctgactga aaggagtttc ctgatgaaag agacgaaaaa catcgatacc gaaaacacgg
     4021 tagttactga cactgtgaaa gaaaccagtg agcgtggcgt aaaacttacc caaccaattg
     4081 agcgaggcgg cgaaaaaatc acgtatgtgg agatcaccgg ggctattgag caggctggat
     4141 ctctgcgaga tttgtcgctg tctgatgtgc tgaatctgaa agcggaatcc atgtttacgc
     4201 tgctgtcacg cgtgacatca ccgcgactgg atgaagtgac gatcaaaaaa atggcatccc
     4261 gtgactttat tcagttatgt gtggttgccg taaatttttt gagcggtgcg gactctggcg
     4321 ggaagaacga acaggcgacg gaagcctgat cacggttgtg tgctttgagt acatagaaga
     4381 ctttgtggcg gatattgccg ccatttttaa ctggtcgccc gccgaaatct tcatgatgac
     4441 gcccggcgaa gtggttagct ggcgtgagcg ggcggcactt cgcagcggga atgcagacaa
     4501 tgaagactct tgatatccgg gtcgctttca gcgccgttga caggctgacc cggcctgccg
     4561 aaaacgcccg ccgcctgatg gggcagtttg gtgactccat ccagcgaact cagggggcga
     4621 tcaaaaatct cgagcgtcag gcgcgttcat ttgagcgcgc ccgtgacgct gtcagtaaag
     4681 cggatgctgg catattgaaa gcacgacgcc agcttaacgc ccttaatcag ttacaacgca
     4741 cgggtacagt gctcagcgaa aaacaacaaa agctgatgca gcagttaagc acccggcttg
     4801 aacgcctgaa tgaatcgcgc acacgggaaa ttcagaaaat gcgggaactt ggcggagagc
     4861 tgaaacgcca cggcatttcc ctgacaggca gcgataacac catccagcag gccatcagac
     4921 gcaccgaaca gtacaacaac cagcttgaac gcgaacggca ggcgcttgcg cgtgtaacgc
     4981 gggcgcgtga gcggtattcg cgcgcgcagg aaacagcggg aaaactgaaa acaggtggtg
     5041 cgctggcaat tggtgcggca gcggcgggcg gctatgctgc cgggcgtttt ttgcagcctg
     5101 cgatcgggtt cggcaaagag atgtcccgcg ttcaggcact gacgcgaatc gaccagaaca
     5161 gcccgcagtt taaggcgctg cgtgagcagg cgttaaaact tggctctgaa acgcagttca
     5221 ccgcaggcga tgccgccagt gggcaggcat ttcttgcaat ggctggcttc acaccacagg
     5281 ccattcaggc tgcgcttccc ggtgtgctga gcatggcaac ggctggcggt atggatctcg
     5341 gcgagacggc ggatattggc tcaaacatcc tgacgcagtt cggcctttct gccgaccaga
     5401 tggaccgggt cggtgacacg ctcaccgcag cgtttacccg taccaacact gaccttcgcg
     5461 cactgggcga aaccatgaaa tatgcaggtc cggtggcggg caagctggga atatcgctgg
     5521 agcaggccgc agcgatggcg ggcgtgctgg cgaatatggg tatcagggga agtgatgccg
     5581 ggacggcaat gcgtgccagc ctggctcgtc tggcatcacc gccaaaggcg gcagcagagg
     5641 cgctgaaaga gcttggtgtg gcagtctctg acgcgaacgg caaaatgcgc ccgatggagg
     5701 atgtgctggc cgacctttat aaagctaccc gcaaatacgg ggaagttgac cgggtatcgt
     5761 tctttaagga cattgccgga gaagaggctt tcacatcgtt tatggcgctc gttgatgcag
     5821 cgggtgacgg ttccttaccc aaactgagaa aagaacttga aggcgcgcgc ggtgaggctg
     5881 aacgcacagc aaaggttatg gccaacaacc ttgacggcga tctgaaatca ctcggcagtg
     5941 catgggaagg gttgcgcatc cgcattgcag atctgattga cggtccgctg cgttctgtca
     6001 cgcagtggct cacgcgggtg gtatcaaggg tgacggcgct ggcgcaggcc catccggcac
     6061 tgacgcgcca gctactgata gcaggcggtg cgttgctggc aatgactgca acggttggct
     6121 cgttgtcgct ggctattggt gtgcttgctg gtccgctggc aaaactgcgt cttggttttt
     6181 ctctcctgac cggatcaatg aatgctgtca ggggcctgcc agcactatgg ggaatggtga
     6241 cgggttccgt ttctttactg ggaggcgcta tcggggcgct gttcagtccg gttggtctta
     6301 tcgtggctgc gcttgccgga gctgccgtcc ttatctggaa atactgggat cccatcaggg
     6361 cattttttgc cggggtgttc agcgggatta tggaaaggct gaccccgttg cgcgaaacct
     6421 ttgaacggtt tggtcctgtt tttgacgcaa tcggaagcgg gataagccag gtgtttaact
     6481 ggtttaaatc gctgctgtca ccgatggagt ccagcaagga aacgctggat aaatgtacca
     6541 gtgctggcga gatattcggt aacgttcttg gcggtgcgtt acagcttgtt ctgacacctg
     6601 caaaaatgct actggatacg ctggcgtgga tacttgaaaa acttggcgtc cttccggatg
     6661 aagcggaaaa ggcgcgcaag aaaatcgaag acgcacagcg tgcggccatt cttcaggaca
     6721 aggttgcctt gcttcagggg gaccttgcga aaatcaatcc gccgaagcct gtggaaaatg
     6781 gcaatggcac cggaggtgat aaacccaaag acaacaaacc gctcacagac agcaataccg
     6841 ggacgctacg cagactcagc aaaattgctg ataacacagg taagctggtt gatgagacga
     6901 aaaaacgcat tggccccggc gatattgtct ttaagaacct gccccgcgca cttgctgttc
     6961 gtggggagtg gcaggagcgg aagattgcac aggtcagtaa gcctgccccc gcaattaata
     7021 tcacccccgt ggtcccggct ccgctgcctc cggcgctggt ccctgttgtt gcggccagct
     7081 cccgcccggt ggcggaggcc atacgatctc cagtggcatc agttcctgta acttcccgta
     7141 accgggagcc tgttgcctcc ggatttggtg gtgaaattca tgttcatctg cataacgttg
     7201 ttacgcagaa tccccgcgaa ctggcgaaac tggtcggtga aatggtcagg gcagaaatgg
     7261 aacggcgcgc ccgtgccggg cgtggcagtt tttacgataa agattgagga gtcatggcca
     7321 tgatgatgat ctatggcatg tttgtttttg agctgcgcac gctgccgcat cagcagttac
     7381 agcaaaacaa aagctggcgg catgtgaaaa atgaacgcgt taaccgttca gcaagctggc
     7441 agtatatcgg tgcaggtgat gatcgcatcg tgctttctgg tgtgctttat cctgaaatta
     7501 caggtggcga agtgtcgctg tcgctgctga ccacgcaggc gtatacagga cggccctggc
     7561 ctctgattga tggcgtcggg cagatttacg gcatgtatgt cctgaccgaa acgaatacga
     7621 cccgctccga gtttgatcgc tacggtaagg cgaaaaagat agaattttca ctgactcttg
     7681 aacgctgtga tgaggatttg cgggagcgcc tgcaatcctc atcgttcagc gatatgttgt
     7741 ccggcttcaa agataaggtg acatcatccc ttaatagcgc gaccagctca gttaaggggc
     7801 tgctctgatt taacgtatgt cgccaatttc ctgatgaagg tgactggcga cttgctgttg
     7861 tatgtccttc tcagaaaatt gtttttgaat aacaaacaac aggattttat aatctcttaa
     7921 ccttataata tgtgtggtct ga
//
LOCUS       DAZGOB010000180         7867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGOB010000180 DAZGOB010000000
VERSION     DAZGOB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.180"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..299
                     /gene="trbF"
                     /locus_tag="ACHLIF_004595"
     CDS             <1..299
                     /gene="trbF"
                     /locus_tag="ACHLIF_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO9608454.1"
                     /translation="FFVFVAAGTFLWVIMHSIFDACIDSWKADPELNNFRYMWNILMY
                     VIPYTLWAFAGGFLIVYVRNPLNELINGGIRIFRLKRRIRRENKLREGKNDATH"
     gene            286..1659
                     /gene="traH"
                     /locus_tag="ACHLIF_004596"
     CDS             286..1659
                     /gene="traH"
                     /locus_tag="ACHLIF_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO9608455.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            1656..4478
                     /gene="traG"
                     /locus_tag="ACHLIF_004597"
     CDS             1656..4478
                     /gene="traG"
                     /locus_tag="ACHLIF_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO9608456.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWSSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWEDNDAHTASSNTQSSHNARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAESILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSVRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGSDSPQELMKRAKEYQDKYKQ"
     gene            4494..4979
                     /locus_tag="ACHLIF_004598"
     CDS             4494..4979
                     /locus_tag="ACHLIF_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO9608457.1"
                     /translation="MIMRCLTHIALVTVIQFIACYLAGWGNAETIFMLFFIVLWLGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGALYELRRVSGCV
                     SVREWMQSQVNEQTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYD
                     E"
     gene            5028..5759
                     /gene="traT"
                     /locus_tag="ACHLIF_004599"
     CDS             5028..5759
                     /gene="traT"
                     /locus_tag="ACHLIF_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO9608458.1"
                     /translation="MKMKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQSKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            6011..>7867
                     /gene="traD"
                     /locus_tag="ACHLIF_004600"
     CDS             6011..>7867
                     /gene="traD"
                     /locus_tag="ACHLIF_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO9608459.1"
                     /translation="MTFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQMEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQA"
BASE COUNT         2013 a         1777 c         2132 g         1945 t
ORIGIN      
        1 tgttttttgt gttcgttgct gccggaactt ttttatgggt gattatgcac agtatttttg
       61 atgcctgtat tgattcgtgg aaagctgacc cggaattaaa taattttcgt tatatgtgga
      121 acatcctgat gtatgtcatt ccgtatacgt tatgggcgtt tgcaggtgga tttcttatcg
      181 tatacgtcag gaaccctttg aatgaattaa taaacggggg gatcaggata ttccggctga
      241 aacgccgtat acgccgggag aacaaattaa gggagggcaa gaatgatgcc acgcattaaa
      301 cctcttctgg ttctttgtgc tgctttactg acggtcacac ccgcagcgtc agcggatgtg
      361 aacagcgaca tgaatcagtt ctttaacaag ctgggcttcg cctccaatac cacacagcct
      421 ggcgtctggc aggggcaggc cgccggttat gcttacggtg gctccctgta tgcccgtact
      481 caggtcaaaa atgttcagct gatttccatg acgctgccgg atatcaatgc cggatgtggc
      541 ggcatcgacg cctatctcgg ctcgttcagt tttattaatg gcgagcagct acagcgtttt
      601 gttaagcaga ttatgagcaa tgctgccggt tacttttttg accttgccct gcagacaacg
      661 gtgccggaaa tcaaaaccgc aaaagacttc ctgcagaaaa tggcaagcga cattaacagt
      721 atgaacctca gttcctgcca ggcggcacag gggattatcg gcgggctctt cccccggacg
      781 caggtgtccc agcagaaagt ctgtcaggac attgccggtg agagcaatat ttttgctgac
      841 tgggcggctt cccggcaggg atgtaccgtt ggcgggaaat ctgacagcgt cagggataaa
      901 gccagcgaca aggataagga gcgggtgacc aaaaacatca acatcatgtg gaatgcgctt
      961 tccaaaaaca gaatgtttga cggcaacaaa gagctgaaag agtttgtgat gacgctgacc
     1021 ggctcactgg tgtttggtcc caacggcgaa atcacaccgc tgtcagccag aaccactgac
     1081 cgctcaatta tccgggccat gatggaaggc ggcacagcaa aaatttatca ctgcaacgat
     1141 tctgataaat gcctgaaagt ggtggcagac acaccggtga ccatcagccg ggataatgca
     1201 ctgaagtctc agattactaa acttctggcc agcattcaga acaaggcggt cagtgacacg
     1261 cctctggatg acaaggaaaa aggctttatt tccagtacca ccatccccgt cttcaaatac
     1321 ctggttgacc cgcagatgct cggtgtttcc aacagtatga tttaccagct gacggactat
     1381 atcggttacg acatcctgct gcagtacatt caggagctga tacagcaggc ccgggcgatg
     1441 gtggccacag gaaattatga cgaagcagtt atcgaacata ttaacgacaa catgaatgat
     1501 gccacccggc agattgcggc gtttcagtca caggtgcagg tacagcagga tgcgctgctg
     1561 gttgtcgatc gtcagatgag ctacatgcgt cagcagcttt ccgcccgcat gctcagtcgt
     1621 taccagaaca actatcactt cggaggaagc acgctgtgaa tgaagtttat gtgattgccg
     1681 gtggtgagtg gttgcggaat aacctgaacg ccattgccgc ctttatggga accaggacgt
     1741 gggattccat tgaaaaaatt gcgctcacat tgtctgttct cgcggtggcc gtgatgtggg
     1801 tacagcggca caacgtgatg gatttgctgg gctgggtggc cgtgtttgtg cttatcagcc
     1861 tgctggttaa tgtccgcaca tcggtgcaga ttattgataa cagtgacctg gtcaaagttc
     1921 accgggtgga taatgtgccg gtcggactgg cgatgccact ttcactgacg acccgtatcg
     1981 ggcatgcaat ggtggccagt tacgagatga tattcaccca gccggacagt gtcacctaca
     2041 gcaaaacggg gatgctgttc ggcgcagaac tggtatcaaa aagcaccgac tttttgtccc
     2101 gcaatccgga aatcgccaat ctttttcagg attatgtgca gaactgtgtg atgggggata
     2161 tttacctgaa ccacaaatac acgctggagg agctgatggc ctccgctgat ccctacacgc
     2221 tgattttttc ccgccccagt ccgctgcggg gcgtttacga cagcaataat aatttcgtga
     2281 cctgtaagga cgcgtcggtt tcgctgaaag acaagctgaa tctggacaca cagagtggcg
     2341 gaaaaacctg gcattattat gcccagcagt tatttggtgg tcgccctgat ccgaacctgt
     2401 tgttcagtac gctgattggc gacagttaca gttacttcta tggctcaagt aaatcagcca
     2461 gccagatcat ccgccagaat gtcaccatca atgcgctgaa ggaggggatc accagttatg
     2521 ccgcacgtaa tggtgattcg gccagcctgg tgaatctggc caccacatca tcgatggaga
     2581 agcaacgtct ggcacatgtc tccatcggtc atgtggcgat gcggacactg cccatgacgc
     2641 agaccatcct gacggggata gccatcggta ttttcccgct cctggtactg gcagctgtct
     2701 tcaacaaact gacgctgtcc gtcctgaaag gctatgtgtt tgccctgatg tggttgcagt
     2761 catggccaat gttgtacgcc attctgaaca gcgccatgac attttatgcg aagcagaatg
     2821 gtgcaccggt ggtgctctcg gaaatttcac agatacagct gaaatactcc gatctggctt
     2881 ccactgccgg gtatctttcc atgatgatcc cgccattatc ctggatgatg gtcaggggac
     2941 tcggggccgg attttccagt gtgtacagcc atttcgcttc ctctgctatc agcccgactg
     3001 ccagtgcggc aggtagtgtg gttgacggta attactccta cggcaacatg cagacggaaa
     3061 acgtgaacgg cttcagctgg agcaccaaca gcaccacgtc gtttggtcag atgatgtacc
     3121 agaccggcag tggcgcaact gccacacaga cccgtgacgg taatatggtg atggatgcga
     3181 gcggggcgat gtcccggtta ccggtcggca tcaatgcaac gcgtcagatt gcggcggcac
     3241 aacaggaaat ggcccgggag gcgtcgaaca gagcagaaag cgccctgcat gggttcagca
     3301 gcagtattgc cagtgcctgg agctcactca gtcagtttgg ttctaaccgg gggagcagtg
     3361 attctgtcac aggtggtgct gacagcacga tgagcgcaca ggactccatg atggccagcc
     3421 gtatgcgcag tgcagtggaa agctatgcga aggcgcataa tatcagtaat gagcaggcga
     3481 ctcaggaact ggcatccaga agtacgagag cttctgccgg gatgtatgga gatgctcatg
     3541 ctgaatgggg ggttaaacct aaaattcttg gtgttggtgg aggattaggc gttagaggtg
     3601 gtggtcgggc tggtattgat tgggaagata atgatgcgca tacggcaagt agtaatactc
     3661 agtcgagcca taatgctcgt catgatatcg atgccagagc tacacaagac tttaaagagg
     3721 ccagcgatta ctttaccagt cgcaaggtca gtgagtccgg cagccatact gacaataatg
     3781 ctgattcccg tgtggatcag ttgtctgcag ccctgaattc agcgaagcag agttacgacc
     3841 agtacacgac gaatatgacc cgcagccatg aatatgctga aatggcatcc cgtactgaaa
     3901 gcatgagtgg gcagatgagt gaagacttgt cgcaacagtt tgcacagtat gtaatgaaac
     3961 acgcgccaca ggatgcagaa tctattttga caaacaccag ttcaccggag attgcagaac
     4021 gtcgtcgggc gatggcttgg tcttttgtgc aggaacaggt acagcctggt gttgataatg
     4081 cctggcggga atcccgtggt gatattggta aaggaatgga gagcgtacct tcgggtggtg
     4141 gcagtcagga tattattgct gaccatcagg gacatcaggc cattattgag caaagaacgc
     4201 aggacagtaa tatccgtaat gatgtaaaac atcaggttga taatatggtc acagaatata
     4261 aagggaatat aggtgatact cagaacagcg tacgtggcga ggaaaatatt gtaagaggac
     4321 agtattctga actgcagaat caccataaaa cagaggcatt atctcagaat aataaatata
     4381 atgaagaaaa gtcggctcag gaaagaatgc ccgggtctga cagtccacag gaacttatga
     4441 aaagggcaaa ggaatatcag gataagtata agcaataatg ctgtttttgg gagatgataa
     4501 tgagatgttt aacccatatt gctctggtaa cggtgataca gtttatcgcc tgttatctgg
     4561 ctggttgggg gaatgccgaa acaattttta tgctgttctt catagtacta tggctgggac
     4621 tgtttatatg gttgttctct caaatacgaa aaaaacggaa tgtgtcggat gagtttaagt
     4681 ttagtaaagg cgtttggtat ataacgatgc cagtatcttc cttactctct ccattattaa
     4741 gcctgatggt ttttatcatt ggtgcactat acgaattaag gcgagttagt ggttgtgtaa
     4801 gcgtaagaga atggatgcaa tctcaggtaa atgaacaaac taatgaagat ttgcatctgg
     4861 attttgataa tatggatttt catcgtacta atcctgcaac agggttacct atgtcaggta
     4921 ttggtgtgga tatcggtggt aattcatatg gttatagttc aaaacgatat gatgagtgaa
     4981 tcttaatttg tatattatga cgttttattc aatatgaagg aacattgatg aaaatgaaaa
     5041 aattgatgat ggttgcactg gtcagttcca ctctggccct ttcagggtgt ggtgcgatga
     5101 gcacagcaat caagaagcgt aaccttgagg tgaagactca gatgagtgaa accatctggc
     5161 ttgaacccgc cagcgaacgc acggtatttc tgcagatcaa aaacacgtct gataaagaca
     5221 tgagtgggct gcagagcaaa attgctgatg ctgtgaaagc aaaaggatat caggtggtga
     5281 cttctccgga taaagcctac tactggattc aggcgaatgt gctgaaggcc gataagatgg
     5341 atctgcggga gtctcaggga tggctgaacc gtggttatga aggcgcagca gttggtgcag
     5401 cgttaggtgc cggtatcacc ggatataatt ccagttctgc cggtgccaca ctcggtgtgg
     5461 gccttgctgc cggtctggtg ggtatggctg cagatgcgat ggtggaagat gtaaactata
     5521 ccatgatcac ggatgtgcag attgcagagc gtactaaggc aacggtgaca acggataatg
     5581 ttgccgccct gcgtcagggc acatcaggtg cgaaaattca gaccagtact gaaacaggta
     5641 accagcataa ataccagacc cgtgtggtct caaatgcgaa caaggttaac ctgaaatttg
     5701 aagaggcgaa gcctgttctc gaagaccaac tggccaaatc aatcgcaaat attctctgat
     5761 tatcttccgg aggctggtct gaccggcctc ctgactgacg cctcgctttg ctcgttgtcc
     5821 aaccccggaa agaaataaaa taaattttat ttctccccgg cgtttccgaa ataaatatga
     5881 aaacactgag cggttgtgca aattctttgt gcgaccgtga agtgttgccg gcgaagccgg
     5941 aatttaacgg ttattaatct tttcatcatt aataatcagt ccgactgact tttttcttcg
     6001 gaatatcatc atgactttta acgcaaagga tatgacccag ggcggtcaga ttgcgtccat
     6061 gcgtatccgc atgttcagcc agatcgccaa tatcatgctt tactgcctgt ttattttttt
     6121 ctggatactc gttggtctgg ttttatgggt aaaaataagc tggcagacgt ttgtgaacgg
     6181 ctgtatttac tggtggtgta ccacgctgga aggcatgcgg gatttaatca agtcccagcc
     6241 ggtatatgag atccagtatt acggcaaaac cttccggatg aatgctgctc aggtactgca
     6301 tgataaatat atgatctggt gcggagagca gctatggtcc gcattcgttc tggccacagt
     6361 tgtggcactg gttatttgcc tgattacctt ctttgttgtc tcctggattc tggggcgtca
     6421 gggtaaacaa cagagcgaaa atgaagtcac aggtggtcgt cagctgacag acaatccgaa
     6481 agacgttgcc cggatgctga aaaaagatgg caaggactcc gatatacgga ttggcgacct
     6541 gccgattatc cgggattccg aaatccagaa cttctgtctg cacggcacgg tcggggccgg
     6601 taagtcggaa gttatccgtc gtctggccaa ctacgcccgt cagcgtggag atatggtggt
     6661 gatttatgac cgttcagggg aatttgttaa aagttactat gatccctcca tcgataaaat
     6721 cctgaatccg ctggatgccc gctgtgccgc ctgggatttg tggaaggagt gcctgacaca
     6781 gccggatttt gataataccg cgaacacgct gatcccgatg ggcacaaaag aggacccgtt
     6841 ctggcagggt tcaggacgta ccatttttgc ggaagcggcg tacctgatgc gtaatgaccc
     6901 caaccgcagc tacagcaaac tggtggacac actgctttcc atcaaaatcg aaaaactgcg
     6961 taccttcctg cgtaattcac cggcggccaa cctggtggaa gagaaaattg agaaaacggc
     7021 tatttccatc cgtgctgtgc tgaccaacta cgtgaaagcc atccgttacc tgcaggggat
     7081 tgagcataac ggtgagtctt ttaccatccg tgactggatg cggggtgtcc gggaagatca
     7141 gaaaaacggc tggctgttta tctcgtcgaa tgccgacacc catgcctccc tgaagccggt
     7201 gatctccatg tggctgtcca ttgccattcg tggtctgctg gcaatgggag aaaaccgtaa
     7261 ccgtcgtgtg tggtttttct gtgatgagct acccacgtta cacaaactgc cggacctggt
     7321 ggagatcctg ccggaagccc gtaagttcgg tggctgttat gtgtttggta tccagtccta
     7381 tgcccagatg gaagatatct acggtgagaa agcggctgcc acgctgtttg acgtcatgaa
     7441 cacccgtgcc tttttccgtt ctcccagcca taagattgca gagttcgctg ccggtgagat
     7501 tggtgagaaa gagcacctga aagccagcga gcagtattcc tacggtgctg atccggtacg
     7561 tgacggggta tcgaccggta aggatatgga gcgccagacg ctggtcagtt attccgacat
     7621 tcagtctctg ccggatctga cctgttatgt caccctgccc ggaccgtatc cggcagtaaa
     7681 actctcactg aaatatcagg cacgtccgaa ggttgcccct gagtttattc cgcgtgacat
     7741 caacccggaa atggagaacc gtctgagtgc cgtacttgcc gcaagggaag cagaaggtcg
     7801 tcagatggcc agcctcttcg aaccggatgt gccggaagtt gtttccggag aagacgtgac
     7861 tcaggct
//
LOCUS       DAZGOB010000181         7827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGOB010000181 DAZGOB010000000
VERSION     DAZGOB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.181"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            84..812
                     /gene="gadW"
                     /locus_tag="ACHLIF_004601"
     CDS             84..812
                     /gene="gadW"
                     /locus_tag="ACHLIF_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO9608460.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1175..4288)
                     /gene="mdtF"
                     /locus_tag="ACHLIF_004602"
     CDS             complement(1175..4288)
                     /gene="mdtF"
                     /locus_tag="ACHLIF_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO9608461.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLCCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4313..5422)
                     /gene="mdtE"
                     /locus_tag="ACHLIF_004603"
     CDS             complement(4313..5422)
                     /gene="mdtE"
                     /locus_tag="ACHLIF_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO9608462.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5530..5808
                     /locus_tag="ACHLIF_004604"
     CDS             5530..5808
                     /locus_tag="ACHLIF_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608463.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5809..6336)
                     /gene="gadE"
                     /locus_tag="ACHLIF_004605"
     CDS             complement(5809..6336)
                     /gene="gadE"
                     /locus_tag="ACHLIF_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO9608464.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7135..7707)
                     /gene="hdeD"
                     /locus_tag="ACHLIF_004606"
     CDS             complement(7135..7707)
                     /gene="hdeD"
                     /locus_tag="ACHLIF_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO9608465.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2080 a         1874 c         1838 g         2035 t
ORIGIN      
        1 ttttgcgcta agaaacagtc tggcaacatt tcattagtat actgaaattg aaataatcgc
       61 agtatgaaat ataagggata atcatgactc atgtctgctc ggtgatcctc attcgtcgtt
      121 cattcgatat ttatcatgaa cagcataaaa tatcgctgca taacgagagt atcgtgctgc
      181 tggagaaaaa tttggcagac gattttgcgt tttgttcacc ggatacgcga cgactggata
      241 tcgatgagct gacagtttgc cattacttac aaaatattcg tcagctacca cgcaatttag
      301 ggttacatag caaagaccgt ctattaatta accagtcacc ccccatgccg ctggtgacgg
      361 cgatttttga tagcttcaat gaatccgggg taaattcacc gatactgagc aatatgctct
      421 acctttcctg tttatcgatg ttttctcata agaaagaact gatcccctta cttttcaata
      481 gcatcagcac tgtttcagga aaagttgaac gccttattag ctttgatatc gccaaacgtt
      541 ggtatctgcg cgatatcgcg gaaagaatgt ataccagcga gagtctcatc aaaaaaaagt
      601 tgcaggatga aaatacctgt ttcagtaaaa tattactcgc ttccaggatg tcgatggcca
      661 gacgattact cgagttacgt caaattcctc tgcatactat tgcagaaaaa tgtggctata
      721 gcagtacgtc gtactttata aacacatttc gacaatatta tggtgtaacg ccacatcagt
      781 ttgcgcaaca ttcgccaggt accttttcct gacatatttt gcatttgaat attggtcagg
      841 atctcacacc tgcttcatgt gaaactcttc cctgatgatt tctgccgggc taccggctag
      901 ttctctttcg cagtttgcca ggcggtatag gtattttcag gaggaaaaga ctcggcatgt
      961 ttgggattat taagctgaca attcatacga ttacgggaat gtgggtcagt attgtgctgt
     1021 tcaaattgat gacgaatggc tggagtggtt tctattttca gtgctgtgta ctaagcctgg
     1081 tattcctgac ggttagctgg ttgttgagtg gtgaatggtt agcaggaaag agtaaggctg
     1141 aaccttcacg ttcaacctta ctctcattta cacgttacgc ttttttaaag cgggcaaaga
     1201 gatgttccac tacaacgaaa aagaccggaa cgaagtaaat tgccagcact gttgcggcaa
     1261 acatcccgcc catcacgccg gtacctaccg cgttttgcgc gccagaaccg gcaccatgac
     1321 tgataaccag cggcagcacg ccgagaataa aggccagaga ggtcatcagg attgggcgta
     1381 aacgcatccg cgccgcttcg atgattgcct ctatcggcgt tttcccttct ttctgcatca
     1441 tctcaacggc aaattcgaca atcaggatgg cgttcttggc ggaaagcccg atggtggtca
     1501 gcaaaccaac ctggaagtag acgtcattac ttaagccgcg cagatcggtg gccagtaatg
     1561 cgccaacgac gcctaacgga acaaccaaca tcaccgagaa cggaattgac cagctctcat
     1621 agagtgcggc gagggcgagg aacaccacga ccagtgaaat ggcatacagt gcaggagcct
     1681 ggtttgagga taacgcttcc tgatacgaca gtccggtcca tgagtagccg acgcctgccg
     1741 gaagtttagc gaccaggtct gccataaatt tcatagcgtc accggtactt ttcccggccg
     1801 ccgcttcacc taaaatctcc attgacggga tgccgttgta gcgttccaat cgcggtgaac
     1861 cataggtcca ttcggtagac gagtaggcag aaagcggtgc catcgtgcca gaggcgttgc
     1921 gtacatacca ttggttgatg ttatccggca acatacggaa cggcgtgcct gcctggacat
     1981 acactttttt cacccgcccc tggttgagga agtcgttcac gtagctgctg ccgaaggcgg
     2041 tggaaattgt ctggttgata tcagacagcg ccacacccat tgcctcagct ttcgcggcgt
     2101 tgacgttcac tttgaacatc ggcgtatctt ccaggccgtt cgggcgtacc ccggtgacct
     2161 ggtcaggcga ttgcgctgcc agtgataaca gctcgtttcg tgcctgggtt agtttttcgt
     2221 gccccaggtt accgttgtcc agcagttcca tatcaaaacc tgacgcggta cccagttcag
     2281 ccaccgcggg taagttgaac gggaagacga cggctttatt gatactgctt aacgcaatca
     2341 ttgcccgctg aatgatcgcg gtaaccgagt tttcctcacc gacacgttca gaccacggct
     2401 tgagactgat aaacgccagg ccgttgtttt gcccctggcc gctgaagcca aagccgccaa
     2461 cggtaaacac cgactgaaca ttatctttct cttttgtaag ataataatcc gtcacctgtt
     2521 gcagcacttt cgtggtgtta accatggtgg caccggaagg taactgcgcg gtggtcataa
     2581 ataccccctg atcctcttct ggtaagaaag aggtcggtgt acgcaggaac agcaccgcca
     2641 tcccggcgca aatcagcagg tagaccacca tgtagcgacc ggtacaacac aacagcgagc
     2701 gggtgctatc ggtatagtgc tgagttgatt tttcaaacag cgtgttgaag cgtgcgaaca
     2761 gggcgttagg tttgtgaccg ccttccggcg cggctttcag aatggtggcg cacagggcag
     2821 gggtcaggct cattgccaca aatactgaaa gcagcatgga ggagatcagc gtgatggaga
     2881 actggcggta gatctccccg gttgcgccgc tcataaaggc catcggcata aacactgcgg
     2941 aaagaacaac agcaataccg accagcgcac gttggatctg ccccatcgat ttatgcgtcg
     3001 cttccttcgg tggtagctta tcttccgcaa tgacacgctc gacgttctcc accaccacga
     3061 tggcgtcatc caccagtaac cctatcgcca gcaccatccc gaacatagtt aacgtgttga
     3121 tggtgaaacc gaccgccgac aagatcgcaa acgtcccgag aataaccacc ggtacggcaa
     3181 tcgtcgggat gattgtggca cggaaattct gcaaaaacag atacatgacc aggaagacga
     3241 ggatgatagc ctcaaccagt gttttaaaaa cttcctgaat agaaatttcg ataaacggcg
     3301 tggtgtcgta aggataaacc gtcttcagac ttgcggggaa ataggctgat aagcggttca
     3361 gctcctcttt gactgcccgc gaggtatcca gggcgtttgc tccggtagcc agtttgatgg
     3421 cgatcccggc agcaggtttg ccgttatagc gcgccacggt ggaataatct tccgctccaa
     3481 gttcgacgcg ggcgacatca cgcagcagca cttgcgaacc atcttgctga actttcaaca
     3541 ggattttgcc aaattcttcc ggcgtttgca gacgcgtctg cacaatgatc gaggcgttta
     3601 gttgctggtc tgccgcctgt ggcatgccac ccagttgacc accggaaatc tggttgtttt
     3661 gcaccttaat ctgggaaata acatcggaag gtaccagatt gtatttattg agtttttgcg
     3721 ggtccagcca gatacgcatg gcgtactcgg aaccaaagag ctgtacgcta ccaacgcccg
     3781 cggtacggct tagcgggtct ttgatattag acgctacata gtccgcgata tcgtactggt
     3841 tgaggctgcc gttatcagaa ataaacgccg ctaccatcag aatattactg ctcgacttat
     3901 caacgctaat cccctgctgc tgcaccgctt caggtaatga aggcatagcg agttgcagtt
     3961 tattttgcac ctgaacctgt gcgatatcag gagatgtccc agtctcgaag gtcagagtga
     4021 tagaggcatt acccgccgca tcactggttg aagacatgta catcaggcca tcaagcccat
     4081 tcatattttg ctcaatcacc tgagtgaccg agtcttctac cgtttgcgca tcggcacctg
     4141 gataggtagc gctgacggta atggtcggtg gcgcaatctg cggatactgc gcaaccggta
     4201 agttcatgat cgccagacca cctgcaagca tcataataat ggcaagtacc caggcaaaaa
     4261 ccgggcgatc aataaaatag ttagccatga aagtcccctt aagcctgcaa cgttattgtt
     4321 tcgattcggt gctggcgttt tcctggctgg aggaaattgc tcgtgctttg atacccggac
     4381 gaatgcgttg caaaccggaa acgatcaccc gatcgccagc ctgcaagcca gaggtgacaa
     4441 cccactggtc gccgatggct ttgctggctt caatttcgcg tagctgcaca acatcgtctt
     4501 tatccagaat gagcgccgtt gctttaccct gggcgttgtg ggtgacgcct tcctgcggca
     4561 ccagtaatac attctggcgg ctaccttcat ccactaatgc cgtgacgtac atgccaggca
     4621 gcaagtcacc atttgggttg gggaaaatcg cccgtaacgt cacggagccc gtggtttcat
     4681 ccaccgtcgg gtcggagaat ttcagcgtgc cggtctggct gtagcgttta ccattttcca
     4741 gattgagctg tactggcgta ctgccctgaa cctgtttgat ttgcccactg gcgacctctt
     4801 ctttcatgcg taagaaatct tgcaccgact gcgtgagatc gacataaatc gggtccagac
     4861 gttgtacggt aaccagcgaa tctgcctgat tagcggtaac gagtgcgccg acggtcaccg
     4921 acgatttccc gctgacgccc gtaatcggcg aggtgacatt cgcgtattgc agattgatcg
     4981 tcgcctgttc aacagccgct ttggcgacgg tgacattggc ttctgcttca ttcaactggg
     5041 tgcgcgcggt atcgtaatcc tgacggctaa cgtagttggt cttcagcaac gatgcctggc
     5101 ggttaaaggt gatgcgggca ttgctggcgg tagagagcgc tttcgccagc gagcctttgg
     5161 cggagtttag ctcggcctgt aaaggtgcag gatcaatctg atacagcgaa tcgccctggt
     5221 tcactttatc gccttcgata aagttgcgtt taatgataat accgcccacc tggggacgta
     5281 tctcggcaac ttcataagga acggttctac cgggcaattc gctcaacaca ttgaccgaac
     5341 cgggggagag tgtgacgaca ccgacctcag gcgtcatggc ggcggcgttt tccgccgatt
     5401 tgtcatcgca ggcggtgagc atcgcgccgc agaataacaa cggtattaac agctttcttc
     5461 ttctgttcat tttagtccct gaaaattctt gagaaataac ggttctttaa attgcgcgcg
     5521 aggagagata tgagggattt acagaccagc ggcatagtgg ggttgtcagc cagtaaggtg
     5581 ggatacaggc acagtgatcg acatggtgag gtcaacgaca aaaaaaatgt tgccgttctg
     5641 ccaacagttc ctgccagcat tcgggcaacg aagggttcta ctggtggata cacataccag
     5701 gggaataaag tcgttttagc ctctccttta gtaatcaccg ggggtaatga aatcagcatc
     5761 tgcatccctc gtcatgccag ccatcaattt cagttgctta tgtcctgact aaaaataaga
     5821 tgtgataccc agggtgacga tgtcgctcat acgtcttagt ttcagcttgt acataatgtt
     5881 gaatttctga atctttaatg tttgttgcga aatattgagc gtcgacgtga tattgctttc
     5941 atttttattt ttcagaatca gttgcataat tttgaactga ttatgtgaaa aacataattg
     6001 tgaattctta tggggcaagt gtttaccata agtaatctca agaagatttt tgatactttc
     6061 tttgcggcta acaaaataaa taggcgcttt agcttttagt ttactgatgt gataaggtgc
     6121 caaaacaata attctctcgg catctaattt ctccagaaat ttaatcgctt cttcatcaag
     6181 gatatgattg tgataggtat caatgataac cttgaagggt ttattgccta cttttttgat
     6241 ctctgacagg gaattgattt ttatcatttc gtgattatct ttcaactgcc aaaagccctg
     6301 taaaagaaaa gaatctttcg tcatgagaaa aatcataact tgctccttag ccgttatcgt
     6361 ttcgaacatg tcatcctttt cattatttac atccttgtcc gaatcgttgt tcaatatagt
     6421 aaacgcctat aagaactttg cccttcgtga actaatacgt ctattaacac taatcaatac
     6481 ttcagattta tccatcacgc actccatata ttgatcctaa gtaagattcc tggttgttat
     6541 caatttgtaa atatcaaaca aaaagttaac gtttgcggat tactggccaa gaataagtta
     6601 tcaataacca gtttgtgatc tctgaagaat attactaaag ttaaaatgta atccgattta
     6661 aatatcgagt ctccttgttt cgacttaagc tggcaattgg attgccagct ttcttttaac
     6721 gcattgaaat tacctgagta aataacgcat tcatgtcttc aggtttttat atagcgtctg
     6781 gagacacgga tatttatgca atgtaatcaa cgccagttgc aaaaaaatga acccggatta
     6841 cttatttaac cgcccatagc atcaacgctt acggaactct ttgttcactt tcccacagca
     6901 cttttaaaac attaaacctg actacggaaa atatcagcca tgataatgtt tgaaatggct
     6961 aatttgccat ggagtgaaaa aaattagatg aaattcagta ggttgaaatg atcactagca
     7021 ggtaattatt tcaatgatag tgcgcaattg atctacaaca ctgcgtagcg gagagagtat
     7081 taatcggatc atagtcacat caagtgacta tgatcctggt gacaaccggg ataattattg
     7141 ctgcttaacg aacaaactgg cgaagctgaa caggctggcg gcgctaaata tcagttcaat
     7201 tcccaccagt gtggaaacca gcgttacaga caccatcggc gttgcaccaa ggaatatcca
     7261 ggcaatgacg atatccagca caccaataac gagctgtagc cagctgcctt tcattgaacg
     7321 ctgacgatac caactcatca ggcgaataac ccctgcaaca cagaacaaac cggcaataaa
     7381 tgccgcaatg gcaaaaatgc ccagctccgg tgcgcggatg aagaaatagc cgatcaataa
     7441 ataggcgact gcgacgagga aaccggataa taccggccag aaattatgac tgcggttgct
     7501 gaataacccg acaataagcg caatacccga gcagattaat aatgcaccca ctactgtgct
     7561 taaaatatcg ccagagacga acgggaaact gatacacagc aacccgacga taaacagcag
     7621 cacggcaata aactggattg ctctgcgatg ctttttcagc atctccagat caaacttcaa
     7681 aattgttgcc ttatctatat ataacataga accaccctat aaaattaaga agaaaatccc
     7741 ctgctatcaa tctatgccaa aaacgcgtct aagaatgcag tcgatttaat aaaaatttcc
     7801 taattgcagt atctgatgca tctgtaa
//
LOCUS       DAZGOB010000182         7717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGOB010000182 DAZGOB010000000
VERSION     DAZGOB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.182"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1335
                     /gene="xanP"
                     /locus_tag="ACHLIF_004607"
     CDS             <1..1335
                     /gene="xanP"
                     /locus_tag="ACHLIF_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO9608466.1"
                     /translation="SELIYRLEDRPPLPQTLFAACQHLLAMFVAVITPALLICQALGL
                     PAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQGTSFNFVAPLIMGGTALKTG
                     GADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPLVSGVVVMIIGLSLIQVGLT
                     SIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNPYLRVASLVIAMAAGYALAW
                     FMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSLETIGDITATSDV
                     SEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYVGF
                     VVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAILI
                     IALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
     gene            1456..3165
                     /gene="yicH"
                     /locus_tag="ACHLIF_004608"
     CDS             1456..3165
                     /gene="yicH"
                     /locus_tag="ACHLIF_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO9608467.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRINGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(3218..5536)
                     /gene="yicI"
                     /locus_tag="ACHLIF_004609"
     CDS             complement(3218..5536)
                     /gene="yicI"
                     /locus_tag="ACHLIF_004609"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO9608468.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGNNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5546..6928)
                     /gene="yicJ"
                     /locus_tag="ACHLIF_004610"
     CDS             complement(5546..6928)
                     /gene="yicJ"
                     /locus_tag="ACHLIF_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO9608469.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            7221..7315
                     /locus_tag="ACHLIF_004611"
     tRNA            7221..7315
                     /locus_tag="ACHLIF_004611"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7255..7257,aa:Sec,seq:tca)
     gene            7394..7522
                     /gene="rhuM"
                     /locus_tag="ACHLIF_004612"
                     /pseudo
     CDS             7394..7522
                     /gene="rhuM"
                     /locus_tag="ACHLIF_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT         1855 a         2050 c         1945 g         1867 t
ORIGIN      
        1 agcgaactga tttaccgtct tgaagatcgt ccgccgcttc ctcaaaccct gtttgccgcc
       61 tgtcagcatc tgctggcgat gttcgttgcg gtgatcacgc cagcgctatt aatctgccag
      121 gcgctgggtt taccggcaca agacacgcaa cacattatta gtatgtcgct gtttgcctcc
      181 ggtgtggcat cgattattca aattaaggcc tggggtccgg ttggctccgg gctgttgtct
      241 attcagggca ccagcttcaa ctttgttgcc ccgctgatta tgggcggtac cgcgctgaaa
      301 accggtggtg ctgatgttcc taccatgatg gcggctttgt tcggcacgtt gatgctggca
      361 agttgcaccg agatggtgat ctcccgcgtt ctgcatctgg cgcgccgcat tattacgccg
      421 ctggtttctg gcgttgtggt gatgattatc ggcctgtcgc taattcaggt tgggttaacg
      481 tccattggcg gcggttacgc agccatgagc gataacacct tcggcgcacc gaaaaatctg
      541 ctgctggcag gcgtggtctt agccttaatt atcctgctta accgtcaacg taacccttac
      601 ttacgcgtgg cctcactggt gattgcgatg gcggccggat atgcgctggc gtggtttatg
      661 ggcatgttgc cagaaagcaa cgaaccgatg acgcaagaac tgattatggt gccaacgccg
      721 ctctattacg gtcttggcat tgaatggagt ctgctgctgc cgctgatgct ggtctttatg
      781 atcacttcgc tggaaaccat tggcgatatc acggcgacct ctgacgtttc cgaacagcca
      841 gtgtccggtc cgctgtacat gaaacgcctg aaaggcggcg tgctggcaaa cggcctgaac
      901 tcgtttgttt cggcggtgtt taacaccttc ccgaactcct gcttcgggca aaacaacgga
      961 gtgatccagt tgactggtgt tgccagccgc tatgtcggtt ttgtcgtcgc gctgatgttg
     1021 atcgtgctgg gtctgttccc ggcagtgagc ggttttgtac aacacattcc agaaccggtt
     1081 ctgggcggcg caacgcttgt aatgtttggc actatcgccg cctccggtgt gcgtatcgtt
     1141 tctcgtgaac cgctgaaccg tcgggcgatt ctgattatcg cgctgtcgct ggcggttggt
     1201 ctgggcgtgt ctcagcagcc gctgattttg cagtttgccc ctgaatggct gaaaaacctg
     1261 ctctcctccg ggatcgccgc gggcggtatt actgccatcg tgctgaatct gattttccca
     1321 ccagaaaaac agtaattcac tggcggcggt aagataatta ccgccgcagt ctcacctctt
     1381 tcaccattcc cgccttgagg attgcgcgta aatcgtgcat aactccctta tgtgcatttc
     1441 acgggatgga agaccatgaa atttattggg aagctgcttc tctacattct catcgctctg
     1501 ttagtggtga tcgctggcct ctattttctt ctgcaaaccc gctggggagc agaacatatc
     1561 agcgcatggg tttccgagaa tagcgactat catctggcct tcggggcgat ggatcaccgt
     1621 ttttccgcgc catctcatat cgtgctggag aacgtcacgt ttggccgtga tggccagccc
     1681 gcgaccctgg tggcaaaaag tgtcgacatt gcgctaagca gtcggcaact gaccgaacca
     1741 cgccatgtcg ataccatcct gctggaaaac gggacgctga atctcaccga ccagaccgcg
     1801 ccgctaccgt tcaaagccga tcgtctgcaa ctgcgtgata tggcgtttaa tagcccgaat
     1861 agcgaatgga aactgagcgc gcagcggata aatggcggcg tagttccgtg gtcaccaaaa
     1921 gccggtaaag tgctgggtac gaaggcgcag attcagttta gtgccggatc gctttcgctc
     1981 aatgatgttc ctgccaccaa tgtactgatt gaaggcagta ttgataacga tcgcgttacg
     2041 ctgactaacc tgggtgccga catcgcccgc gggacattaa ccggaaacgc gcagcgtaac
     2101 gccgacggca gctggcaagt ggaaaacctg cgcatggcgg atatacgtct acaaagcgaa
     2161 aaatcgctaa ccgacttctt tgcgccatta cgctctgtcc cgtcgttgca gattggtcgc
     2221 ctggaagtga tcgatgctcg tttgcaaggt ccggactggg cggtgaccga cctcgatctc
     2281 agcttgcgca acatgacctt cagtaaagat gactggcaga cacaggaagg caaactgtcg
     2341 atgaacgcta gcgagttcat ttatggttcg ctgcatttat ttgacccgat tataaacgcg
     2401 gaattttccc cgcagggcgt agcgctgcgc cagttcacca gccgctggga agggggtatg
     2461 gtcagaacgt cagggaactg gctgcgtgac gggaaaacgt tgatccttga tgatgtggca
     2521 attgccgggc tggaatatac cttgccgaaa aactggcaac agttgtggat ggaaacgaca
     2581 cccggttggt taaacagcct gcaactgaag agatttagcg ccagccgcaa tctgatcatt
     2641 gatatcgacc ctgacttccc gtggcagctc accgcgctcg atggttacgg tgccaacctg
     2701 acgctggtta ccgatcataa atggggcgtc tggagtggct cggcgaatct gaatgccgcc
     2761 gccgcgacat tcaatcgtgt tgatgttcgt cgcccgtcgc tggcgctgac cgccaacagc
     2821 agcacggtga atatcagcga actgagtgca tttactgaaa aaggcattct ggaagccact
     2881 gccagtgttt cacaaacgcc acaacgtcag acccatatca gcctgaatgg acgcggtgtg
     2941 ccggtgaata ttttgcaaca atggggatgg cctgaattac cgttgactgg cgacggcaat
     3001 attcagctta ccgccagtgg agatattcag gccaatgccc cgctgaaacc tacggttagc
     3061 gggcaattgc atgccgtgaa cgccgcaaag cagcaagtga ctcaaaccat gaatgctggc
     3121 atcgtttcca gcggtgaagt tacgtcggca gagctggtgc agtaaacatc aatctcgccg
     3181 ggtacgactc agacgtaccc ggcattccat caatagatta caacgtaatt gtcagcgcat
     3241 tcccttgagg cttcaccacc agcccctgct cactttcagc ctgcgaaccg tcttgcagac
     3301 catttacttt cacaacattg cgcaggcaca gtgtccagtt cttcgcctcg cccgcaccag
     3361 tcacagtaat cgtgttgcca gtacgtgctg cttttaaagt aaagatcacc gatccgtcag
     3421 cagcgggcac ttcacagacg gcttcatgcc cgtcttgcag attaaagagg tggaatgccg
     3481 tgccttcgtg ccacacgtaa tcgggacgtt gatcgttgtt gcccagcgcc agtagagtgt
     3541 tatcacgcac ataaacgggc agactcagga agccgtgctg ctgtttatgc cagcgactac
     3601 cgtcgagttc atcgttgtgc cacaggtgtg tccagcgacc ttccggcagg tagaactgca
     3661 catcgcccgc ttcagtgaac accggcgcaa ccatcacgtt gttgcctaac atgtattgac
     3721 ggtcaaggta atcacaagcc ggatcgtccg ggaactccat catcatggcc cgcatcatcg
     3781 gcgtaccccg cgcgttcgca cgcgcagctt cacgatacag atacggcatc atgcggcatt
     3841 tcagttgcgt gaagaagcgc actacatcac aggactcatc atcgtaggcc cacggcacac
     3901 gataagattt gctaccgtgt aaacggctat ggctggagag caaaccaaac gcgcaccagc
     3961 gtttgtaaac gtgcgccgga gcggtatttt caaagccgcc gatatcgtgg ctccagaagc
     4021 caaaacctga aaggccaata gacaaaccac cgcgcaggct ttccgccatt gattcgtagt
     4081 tagcgtaaca atcgccaccc cagtgtaccg ggaatttctg cgcaccgacg gaggccgagc
     4141 gggcaaacaa gacagcttct tcctcaccaa cggtgtcctt gagcacgttc cacaccagtt
     4201 cgttgtagat gtatgcataa tggttatgca ttttctgcgg atcggaaccg tcaaaccact
     4261 gaacatcagt tgggatacgt tcgccaaagt cggtcttaaa gcaatcaacg cccatcgcga
     4321 ccagaccttt cagtttgtcg gcgtaccatt tgcaggcatc cggattggta aagtcataaa
     4381 tcgccagacc tggctgccat ttatcccact gccatagcga accgtccggg cgtttgagta
     4441 aatagccttt ctcttgtaac tctttaaaga cgggggattt ttgaccgata taggggttaa
     4501 tccagacgca gattttcagc cctttcgctt tcaggcggcg gatcatccct tccgggtcag
     4561 ggaaagtcag cgggtcccac tcaaaatcgc accactggaa ggctttcatc cagaaacagt
     4621 caaagtggaa aacatgcagc ggcagattgc gttccgccat accatcgata aagctgttta
     4681 ccgtcgcttc gtcgtagttg gtggtaaatg aagtggttag ccacaggccg aaggaccacg
     4741 cgggcggcag cgccggacga ccagtaaagc gggtataacg atcaagtacc gctttcggcg
     4801 tcgggccgtc gataacaaag tattcgagat attcactctc aacgctgaac tgcactttgg
     4861 agactttctc cgatcccact tcaaaagaga cacactgggg atgattgacc agtaccccat
     4921 aaccacggtt agtcatgtag aacgggatat ttttatacgc ctgttcagta cttgtgccgc
     4981 cgtcccggtt ccaggtctct accgtctggc cattgcgcac cagggcagta aagcgctctc
     5041 ccagaccgta aactgtttcg ccaacgccaa gatcaagccg ctcaaacata taattgcgtt
     5101 gattattcgt gtcctgcacg tagccattat ttttcacctg actaccggta atacgttcgc
     5161 cgttgcgcag aaaatccagt gaccagaact cacctttgct gacacgcgcg cttaagttgc
     5221 cacttttaaa ctcagcataa cgttctgtgt tttcgattgt gaccttcacg tcctgcaaaa
     5281 tattgagcgg ataatgagga ccgttattca gcgccccctg aaaatgctca atccgcacac
     5341 cgacaatacc ttcctgtggg gagaaaaagc gcagcgtaaa taaaggcgta tcaagctgcc
     5401 aggtacgttc acgcacatca cggggggcag catagaccac catttcatta ccctgctgtt
     5461 caacctcgaa tacctgaagc gggtgaatca aattgaggcc aggttgaatc aaccagtttc
     5521 catcgctaat tttcattgcc gttccttagt tctgcacttc ttgagaggtg aattgttgct
     5581 ggcaacggcg tttaccctga gccagctgtt ccataacggt tttcagattg tgcgtcgtga
     5641 gtgagtagta gcgtttagcg ataatcgcgc tcagcaaata acagatcgcc ggaacaatcg
     5701 tgaatagcgc aataatgatg ctaatcgtgg cgctgttctg cgctttttct gccgcatcat
     5761 atccgccata agccagcatc cagccgataa gagcgccgcc gaaggccaac cccagtttga
     5821 gcacaaacag cgtgccagca aaactgatcc cggtcaggcg tttaccattg caccactcgc
     5881 cgtagtcgac ggtatcggac atcattaccc actggatagg tgtcaccagt tgatgcaaca
     5941 caccaatcac gaagatgaag acaaacatag tgatgctggc ctgcatggga acaaagaaca
     6001 tcgcgaggct aatcactgcc agcagggcgt tcgtccacca gaagatagtg actttacatt
     6061 tccagtcggt cagaggtttt gccagtgcgg aaccaatcag gttacccacg caataagtgg
     6121 tgagaaaagc gacaaacact tccggcgtgc ccaaaatcca tgtgacgtaa tacatcatcg
     6181 ccccaccgcg tacgcacacc gccaggatat tgaaaatggt tagtaaaccg acaatccgcc
     6241 actggtcgtt ttgccagata tcacgtaaat cttcccgcat agacgttgtt gtaggtggtg
     6301 cttcaacgcg ttctttagtg gtgaagaaac agaatgccag catcatgaat gccaccacgg
     6361 aaaggaccgc gataccgccc tggaaaccga gtggtttatt atcaccgcca attaaattaa
     6421 ccagtggcat catcagaaca gtagaaagca tgcctcccgc ggtcgccagc acaaaacgcc
     6481 aggattgcag cgagatacgc tgagtcgggt cattggtgat tacaccaccc aatgcgcagt
     6541 aagggatatt gacgacggta taaagtaagg taagtagggt gtaagtaatt gctgcataga
     6601 tcattttgcc gttcatactg agatctggcg tgctataggc cagtacacag acgatcccga
     6661 atggcagtgc gccaaacagt acccacggac gaaatttacc ccagcgagag cgcgttcgat
     6721 cggccaacaa ccccatgcaa ggatcggaaa tcgcatccag tgcacgagcg accaaaaaca
     6781 tggttccgac aaaaccggca ggaatgccaa aaatatcggt ataaaagaac atcatatata
     6841 acattacgtt atcgaaaata atgtggctgg cggcgtctcc catgccataa ccaattttct
     6901 ctttaacgga caacacttca ctcttcatct gcttttcctt gatgctatag gggtgctgag
     6961 accgataacg cattttgtaa accatccctg tgagagcgaa tattccgata tctggttatt
     7021 aatttatgat tcttgtttta tgtgatcgtg gtagcgttaa tttcgctcat atatcattgt
     7081 aaaatatagg ttttatatga actataatgc tttcgtgata atacgctgcg tgtattaggc
     7141 ggaaaaaact gatctggggg atgtagaaac tcaaggaagt agctataatg cgccccgcct
     7201 ccatgtagca atcgaggcgc ggaagatcgt cgtctccggt gaggcggctg gacttcaaat
     7261 ccagttgggg ccgccagcgg tcccgggcag gttcgactcc tgtgatcttc cgccaaaatg
     7321 cctcttctga cgttctccaa ccccttgtaa tactgtcaat ccagatattt tctactgctc
     7381 aatattcaat cggcttcagg aatacctggt ctaaggtttt gtgatggacg atgagcggct
     7441 gaagaatccg cctgtcggtt cttctgtcct cgaacgcatc cgcgatatgc gcgccagcat
     7501 gtgccgtgtt tatttgcagg tgatttgccc cctgctggct ctcagccaac tgtatcgcca
     7561 ttaaggtgaa gatgttgttt tactcgcccc agaacaccct tttttatacg actattctat
     7621 ttctttaacc gctggcgaaa aaaactgact ataaacttca ccaataatca gactgccatc
     7681 aagattaatt tgttcattta atatatcaaa tcaactt
//
LOCUS       DAZGOB010000183         7579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGOB010000183 DAZGOB010000000
VERSION     DAZGOB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.183"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            141..1538
                     /gene="ulaA"
                     /locus_tag="ACHLIF_004613"
     CDS             141..1538
                     /gene="ulaA"
                     /locus_tag="ACHLIF_004613"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO9608470.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGYIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1554..1859
                     /gene="ulaB"
                     /locus_tag="ACHLIF_004614"
     CDS             1554..1859
                     /gene="ulaB"
                     /locus_tag="ACHLIF_004614"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO9608471.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1869..2333
                     /gene="ulaC"
                     /locus_tag="ACHLIF_004615"
     CDS             1869..2333
                     /gene="ulaC"
                     /locus_tag="ACHLIF_004615"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO9608472.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2347..2997
                     /gene="ulaD"
                     /locus_tag="ACHLIF_004616"
     CDS             2347..2997
                     /gene="ulaD"
                     /locus_tag="ACHLIF_004616"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO9608473.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            3007..3861
                     /gene="ulaE"
                     /locus_tag="ACHLIF_004617"
     CDS             3007..3861
                     /gene="ulaE"
                     /locus_tag="ACHLIF_004617"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO9608474.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3861..4547
                     /gene="ulaF"
                     /locus_tag="ACHLIF_004618"
     CDS             3861..4547
                     /gene="ulaF"
                     /locus_tag="ACHLIF_004618"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO9608475.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4676..4951)
                     /gene="yjfY"
                     /locus_tag="ACHLIF_004619"
     CDS             complement(4676..4951)
                     /gene="yjfY"
                     /locus_tag="ACHLIF_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO9608476.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5278..5673
                     /gene="rpsF"
                     /locus_tag="ACHLIF_004620"
     CDS             5278..5673
                     /gene="rpsF"
                     /locus_tag="ACHLIF_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO9608477.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5680..5994
                     /gene="priB"
                     /locus_tag="ACHLIF_004621"
     CDS             5680..5994
                     /gene="priB"
                     /locus_tag="ACHLIF_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO9608478.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5999..6226
                     /gene="rpsR"
                     /locus_tag="ACHLIF_004622"
     CDS             5999..6226
                     /gene="rpsR"
                     /locus_tag="ACHLIF_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO9608479.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6268..6717
                     /gene="rplI"
                     /locus_tag="ACHLIF_004623"
     CDS             6268..6717
                     /gene="rplI"
                     /locus_tag="ACHLIF_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO9608480.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6788..>7579)
                     /locus_tag="ACHLIF_004624"
     CDS             complement(6788..>7579)
                     /locus_tag="ACHLIF_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO9608481.1"
                     /translation="TLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDHN
                     CDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHKATLDNIHAQKYN
                     KAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQ
                     MPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSA
                     AIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         1813 a         1833 c         2071 g         1862 t
ORIGIN      
        1 ctatccgtgt ggaattattt gcgggtcgcg tcacatttaa tcataaataa tcttgttgtg
       61 attacttttg aaaattagag tgagtgcaca acattccggg tgtgcggaat acccggttac
      121 ctcttcttca ggagatcgtt atggagatcc tctacaacat ctttaccgtg ttttttaacc
      181 aggtcatgac caatgccccg ttgttgctgg gtattgtgac ctgtctgggc tacatcctac
      241 tgcgcaaaag tgtcagcgtt attattaaag gcacgattaa aaccataatt ggtttcatgt
      301 tgttgcaggc agggtccggc atcctcacca gcaccttcaa accggtggtg gcgaaaatgt
      361 ccgaagtcta cggcattaac ggcgcaattt ccgatactta cgcgtcgatg atggcaacca
      421 tcgaccgcat gggcgatgcc tatagctggg taggttacgc ggtgttgtta gcgctggcgc
      481 tgaacatctg ttacgtgctg ctgcgtcgca ttaccggtat tcgtaccatc atgctgactg
      541 gttatatcat gttccagcag gcggggctga ttgccgttac gttgtttatt ttcggctact
      601 ccatgtggac aaccattatc tgcacggcga ttctggtttc gctctactgg ggcattacct
      661 ccaacatgat gtacaagccg actcaggaag tgacggacgg ctgcggtttc tccatcggtc
      721 accagcaaca gtttgcatca tggattgcct ataaagttgc gccgttcctc ggcaaaaaag
      781 aggaaagcgt tgaagacctc aaactgccag gctggctgaa tattttccac gacaacatcg
      841 tctccacggc gattgtgatg accatcttct ttggtgccat tctgctctcc ttcggtatcg
      901 acaccgtgca ggcgatggca ggcaaagtga actggacggt gtacatcctg caaactggtt
      961 tctccttcgc ggtggcgatc ttcatcatca ctcagggcgt gcgcatgttt gtggcggaac
     1021 tctctgaagc atttaacggt atctcccagc gcctgatccc tggtgcggtt ctggcgattg
     1081 actgtgcggc tatctatagc ttcgcgccga acgccgtggt ttggggcttt atgtggggca
     1141 ccatcggtca gctgattgcg gttggcatcc tggtcgcctg cggttcctcg atcctgatta
     1201 ttcccggctt tatcccgatg ttcttctcca acgccaccat cggcgtgttc gctaaccact
     1261 tcggcggctg gcgtgcggcg ctgaagatat gtctggtgat ggggatgatt gaaatctttg
     1321 gctgcgtctg ggcggtgaaa ctcaccggta tgagcgcctg gatgggcatg gcggactggt
     1381 cgattctggc accgccgatg atgcagggct tcttctccat cggtatcgcc tttatggccg
     1441 tcatcattgt aattgcactg gcttatatgt tcttcgctgg ccgcgcactg cgcgcagaag
     1501 aagatgcaga aaaacaactg gcagaacagt ctgcttaata aggagttttg attatgaccg
     1561 tacgtattct ggctgtgtgt ggcaacggac aaggcagttc catgatcatg aagatgaaag
     1621 tggaccagtt tttaacccaa tcaaacattg accacacggt aaatagctgc gcggttggcg
     1681 aatacaaaag cgagttgagc ggcgcggata tcatcatcgc ttctacgcac attgcgggcg
     1741 aaatcaccgt gaccggcaac aaatacgtgg ttggcgtgcg caacatgctc tctcctgccg
     1801 actttggccc gaaactgctg gaagtgatca aagcgcattt cccgcaggat gtgaagtaag
     1861 gacgcgccat gaaattacgt gattcgctgg cggagaataa atccatccgc ctgcaggctg
     1921 aagctgagac atggcaggac gcagtaaaaa tcggcgttga cctgctggtg gcggcggatg
     1981 ttgtcgagcc gcgttactac caggcgattc tggatgcggt tgaacagcac ggtccgtact
     2041 ttgtgctcgc accaggcctg gcaatgccgc acggtcgccc ggaagagggc gttaagaaaa
     2101 ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt caaccacgaa gataacgacc
     2161 cggtggatat cctcatcacc atggcggcgg tcgatgccaa cacgcaccag gaagtgggca
     2221 tcatgcagat cgtcaacctg tttgaagatg aagaaaattt cgaccgttta cgtgcctgcc
     2281 gtaccgagca ggaagtactg gatctcattg accgcaccaa cgcggcagct taagaaggaa
     2341 ttgaagatgt cattaccgat gttgcaagtc gcgctggaca accagactat ggatagcgcc
     2401 tacgaaacca ctcgcctgat tgccgaagaa gtcgacatta tcgaagtggg taccattctg
     2461 tgcgtgggcg aaggcgtgcg tgcggttcgt gacctgaaag cgctctaccc gcacaaaatc
     2521 gtactggcag acgctaaaat tgctgatgcg ggcaaaatcc tttcgcgtat gtgcttcgaa
     2581 gccaacgctg actgggtgac ggtaatttgc tgtgcggata tcaacaccgc caaaggcgcg
     2641 ctggacgtag caaaagagtt taacggcgac gtgcagatcg aattgaccgg ctactggacc
     2701 tgggaacagg cgcagcagtg gcgtgacgca ggcattcagc aggtggttta tcaccgcagc
     2761 cgtgacgcgc aggccgcagg cgtagcgtgg ggcgaagcgg acattaccgc gatcaaacgt
     2821 ctttccgata tgggcttcaa agtcaccgtc accggtggcc tggcgctgga agatctgccg
     2881 ctgttcaaag gtattccgat tcatgtgttt atcgctggtc gcagcatccg tgatgccgct
     2941 tctccggttg aagctgcacg tcagttcaaa cgttccatcg ccgaactgtg gggctaagga
     3001 gcggatatgt tgtccaaaca aatcccgctt ggcatctatg aaaaagcgct ccccgccggg
     3061 gagtgctggc tggaacgcct gcaactggca aaaacgttgg gcttcgattt tgtcgagatg
     3121 tcggtagatg aaacggacga gcgcctgtcg cgcctcgact ggagccgcga gcagcgtctg
     3181 gcgctggtga atgcgattgt tgaaaccggc gtgcgcgtgc cgtccatgtg cctttctgct
     3241 catcgtcgtt tcccgctggg cagtgaagat gacgcggtgc gggcgcaggg gctggagatt
     3301 atgcgtaaag ctatccagtt cgcccaggac gtcggcattc gcgtgatcca gctggcgggc
     3361 tatgacgttt actatcagga agccaataac gaaacgcgtc gtcgtttccg tgacggcctg
     3421 aaagagagcg ttgagatggc gagccgcgcg caggtgacgc tggcgatgga gatcatggat
     3481 tatccgttga tgaactccat cagcaaggcg ctgggttacg cgcactatct caacaacccg
     3541 tggttccagc tctacccgga tatcggcaac ctgtcggcgt gggacaacga tgtgcagatg
     3601 gagttgcagg ccggaatcgg gcatatcgtc gcggtacatg tgaaagacac caaacctggc
     3661 gtcttcaaaa acgtgccgtt tggcgaaggt gtagtggatt tcgaacgttg tttcgaaacg
     3721 ctcaaacaga gtggctattg cgggccgtac ctgattgaga tgtggagcga aacggcggaa
     3781 gacccggcgg cagaagtggc gaaagcgcgt gattgggtga aagcgcgcat ggcgaaagcg
     3841 ggcatggtgg aggcggcata atgcaaaagc taaaacagca ggtatttgaa gccaacatgg
     3901 atctgccgcg ctacgggctg gtgaccttta cctggggcaa cgtcagcgct atcgaccgcg
     3961 aacgcgggct ggtggtgatc aagcccagcg gcgtcgccta cgaaaccatg aaagcagacg
     4021 atatggtggt ggtcgatatg agcggtaagg tggtggaagg ggagtatcgc ccatcttccg
     4081 acaccgcgac gcatcttgaa ctctaccgtc gttatccgtc gcttggcggc attgtccata
     4141 cccactccac tcatgctacc gcatgggcgc aggcggggct ggcgatcccg gcgttaggca
     4201 ccacgcacgc tgactacttc tttggtgaca tcccgtgtac gcgtggatta agcgaagaag
     4261 aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat tatcgaaacg ctgggcaacg
     4321 ccgagccgct gcatacgccg ggaattgtgg tgtatcagca cggaccgttc gcctggggga
     4381 aagatgccca cgacgcggtg cataacgcgg tagtgatgga agaagtggcg aaaatggcgt
     4441 ggattgcgcg cagtattaat ccacaactca atcacatcga cagcttcctg atgaataaac
     4501 acttcatgcg taagcatggt cctaacgctt attacgggca gaagtaaaac acgcgctgcg
     4561 gaaatttcct tcttcgggag ataactggtc taattccgca gccgtttttc aaaaaaagcc
     4621 ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt tggttatacc tggtgctagc
     4681 gataaatatc cgcgctggcg tgcatattgc cgttactccc cggttcccgc atcagaatta
     4741 cgtggtagta cgttgcgccc tgcgcatctg tttcttcatt taatgcctga cgtgcttcac
     4801 tttcagtggc gaaattatga ttgatataaa tcacgcctaa gctttgcaca tcgtccatat
     4861 ttctggcctg gtggttattg atttcaatgg ctgcccatgt atttgcactt agcaaaagca
     4921 cagccagaag ggctaaaaca cgactgaaca tagatacctc ctcaacggct gactttgtgt
     4981 gctctcctct gtgatgatct tctgatttaa ttttaatcaa tgataaagaa gttgatggtg
     5041 accatttctg atgcagttgt tcgaaaaaac actgtgacgg aatgtgatga acttcaaatc
     5101 agggagttag ttcttttgag cgaaagatga gatttatttg tcggctggcc ttgagtttag
     5161 cggggcatac aagtactata acgcgtcatt tttcagccga cctttaacac gttccttgcc
     5221 tccccgggat tcggctgacc cagacaggag gctgaataat ccgtaaggag caattcgatg
     5281 cgtcattacg aaatcgtttt tatggtccat cctgaccaga gcgaacaggt tccgggcatg
     5341 atcgagcgct acactgctgc catcactggt gcagaaggca agatccaccg tctggaagac
     5401 tggggccgcc gtcagctggc ttacccgatc aacaaactgc acaaagcaca ctacgttctg
     5461 atgaacgttg aagctccgca ggaagtgatc gatgagctgg aaactacctt ccgcttcaac
     5521 gatgccgtta tccgcagcat ggttatgcgt accaagcacg ctgttaccga agcatctccg
     5581 atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg atttcgcaaa cgaaaccgct
     5641 gatgatgctg aagctgggga ttctgaagag taatttctga tgaccaaccg tctggtgttg
     5701 tccggcaccg tgtgcaggac tccccttcga aaggtcagtc catcaggaat tcctcactgc
     5761 cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg gctttcaccg gcaggcatgg
     5821 tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg ccattactca cagtataacg
     5881 gtcggcagtc gcataaccgt tcaggggttc atttcatgcc acaaggcaaa gaacggactg
     5941 agcaaaatgg ttttgcatgc cgagcagatt gaattgatag attctggaga ctagccatat
     6001 ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg ttcaagagat
     6061 cgactataaa gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta agattgtccc
     6121 aagccgtatc accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg ctatcaaacg
     6181 cgctcgctac ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg tcacggtcca
     6241 ttaatacgac tttgagagga taaggtaatg caagttattc tgcttgataa agtagcaaac
     6301 ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa cttcctggta
     6361 ccgcagggta aagctgttcc agctaccaag aaaaacattg aattcttcga agcacgtcgc
     6421 gctgaactgg aagctaaact ggctgaagtt ctggcagctg ctaatgctcg cgctgagaaa
     6481 atcaatgcac tggaaactgt taccatcgcg tctaaagctg gcgacgaagg taaactgttc
     6541 ggttccatcg gtactcgcga catcgctgac gctgtaactg cagctggcgt tgaagtggct
     6601 aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca ctggcgaaca cgaagtgagc
     6661 ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc tgaataattc
     6721 gttattcaac gagacgtaaa aacgccgacc attggtcggc gttttgcttt ctatttttcg
     6781 tcaggtatta gtttcgcaag tagatccatt caccattctc ttgtgtccat aagtcaggat
     6841 ggtttgctgg cgttagatgc atacgatcac tccttatgtc tcggtcctgc ggcactcgct
     6901 cgaaacctaa acccgagtag attgctgctg aatttgataa ggaacgtaga ccaattttca
     6961 aatcatgggg taaatcagaa tacgcgttta gcaatgcagc aatgatgaat ccgagggcat
     7021 tagaaacgcc tgtaacagtg cgaagttggt cgatataaag atcgattgtt ggtagttcag
     7081 atgaccagca attaggcatt tgggcaggaa gggttacaac gtatcctatc gtttggccgg
     7141 tatagtctgg agtaccataa ccaggtccca aaaccacttc gtgtattgtg tatttactgt
     7201 tgggttcgaa ggattcgaca tcttgcaata aatatttcag ttctgcggtt aacctcgcac
     7261 ccgcgtgagc tttattgtat ttctgggcgt gaatattatc taatgtagct ttatggttcc
     7321 atccaaaaga attatcacca ttcattgtta ctccatcgta agcattacac ttcgttgcta
     7381 acttattgaa tgcttgccag tcagagagat ctagtttgtg gatctcaaca gtacgtctgc
     7441 catcgcaatt atgatctctt ccacgatttg gcagtaatgt agcacgtgca tattgatgaa
     7501 attttaattt tactggtttg tcggtggacg aaaaatgggg tgaatatgca ggaaatgaat
     7561 aaatagtcgt tggtaatgt
//
LOCUS       DAZGOB010000184         7421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGOB010000184 DAZGOB010000000
VERSION     DAZGOB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.184"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(63..886)
                     /locus_tag="ACHLIF_004625"
                     /pseudo
     CDS             complement(63..886)
                     /locus_tag="ACHLIF_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(940..2658)
                     /gene="proS"
                     /locus_tag="ACHLIF_004626"
     CDS             complement(940..2658)
                     /gene="proS"
                     /locus_tag="ACHLIF_004626"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO9608482.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2770..3477)
                     /gene="tsaA"
                     /locus_tag="ACHLIF_004627"
     CDS             complement(2770..3477)
                     /gene="tsaA"
                     /locus_tag="ACHLIF_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO9608483.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3474..3878)
                     /gene="rcsF"
                     /locus_tag="ACHLIF_004628"
     CDS             complement(3474..3878)
                     /gene="rcsF"
                     /locus_tag="ACHLIF_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO9608484.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3996..4811)
                     /gene="metQ"
                     /locus_tag="ACHLIF_004629"
     CDS             complement(3996..4811)
                     /gene="metQ"
                     /locus_tag="ACHLIF_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO9608485.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4851..5504)
                     /locus_tag="ACHLIF_004630"
     CDS             complement(4851..5504)
                     /locus_tag="ACHLIF_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO9608486.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5497..6528)
                     /gene="metN"
                     /locus_tag="ACHLIF_004631"
     CDS             complement(5497..6528)
                     /gene="metN"
                     /locus_tag="ACHLIF_004631"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO9608487.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6716..7291
                     /gene="gmhB"
                     /locus_tag="ACHLIF_004632"
     CDS             6716..7291
                     /gene="gmhB"
                     /locus_tag="ACHLIF_004632"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO9608488.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1814 a         1925 c         1888 g         1794 t
ORIGIN      
        1 ttttgtaggc cggataaggc gtttacgccg catccggcat tttgtgcacg aggctggctt
       61 tatcactgag tcagcccaac aaagcgaccc gccttgatat aaatcccagg cttcaggtgc
      121 ccaacatact gtaactgcgt ttctggctta aacgcagaga catcaccaat acgcatatgc
      181 atcagatcgg cagggctaat ccagcccgat tgcgccagtg taagtggatg cccggctttg
      241 gcaaaggcat ccgtgacgaa ttccgaacaa aaccacgact ttttgtctcc ttcgcccaca
      301 ctgcttaact gcgctttcgc caggccgctg acgcactttg gcgaaaatcc tcggagaacg
      361 gattcagtga gcacatctgg cgagtcacca taaagggaat aaattcgaca atgccgcgat
      421 agttataacc gctatctttg attttattgg caaacgcggt gatatctgtg gcttgttgcg
      481 gggtaagatc cgggactcgt aagacgaaaa gcttatcact atgcttcatc gcttttttaa
      541 gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac gttattatca cccagaaaga
      601 ttgcaacgtg actcacagag gaagtgctga agacgcggat tccaaatgag gttaccccaa
      661 ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg tgccgttatt tcttttattg
      721 attgttcggt gaaagagctt tgatgctgga atttgacagc ccatgttttt gcctcggcat
      781 ctactgctgt ggctgatggg tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa
      841 gaaagctggg gagaaacagg cggcagtacg cctttggttt atccatttta tacaatccat
      901 gtaaaaaaag ggccctgaaa ttcaggaccc tttctggcat cagcctttaa tctgtttcac
      961 cagatattcg acgatgtcac cagtcttaat taactgtttc tcgccgttgc gacgatattt
     1021 atattcgata tcgtcgttgt cgaggttacg gtcgcccagc acaatagtgt gcggaatacc
     1081 gatcagttcc atatcagcaa acatcacgcc cggacgctct ttgcggtcat ccagcagcac
     1141 ttcgatacct tgtgcacgca gttcgctgta cagtttctca gcaagctctt gtacgcggaa
     1201 ggatttgtgc atgttcatcg gcagaatcgc cacctggaac ggcgcgatag cgtcaggcca
     1261 tacgatgcct cgttcgtcgt agttctgctc aatcgccgca gctaccacac gcgttacccc
     1321 gataccgtag caacccatcg tcaggatttg gttacggcca tcttcaccct gtacggaggc
     1381 tttcagtgct tcggagtact tggtacccag ctggaagatg tgaccaactt cgataccacg
     1441 tttgatcagc agcgtaccct ggccatccgg gcttggatcg ccagccacca cgttacggat
     1501 atctgcaact tccggggtag cgacatcgcg atcccagttg atgccgaagt agtgtttacc
     1561 atcgatgtta gcaccagcag cgaaatcact catcgccgca acggtacggt caatcaccac
     1621 cggaatcggc atgtttaccg gacccagtga acccggaccg gctttaacca cggcacgaat
     1681 ttcttcttcg gtcgcgaaag tcagcgggct tgcaacctgc ggcagttttt ctgctttaac
     1741 ttcgttcagc tcgtgatcac cgcgcaccag cagcgcaacc agcgggaagc tactgccttc
     1801 aaccgcttta accagcagag tcttaaccgt tttctcaatc ggcagattga actgttcaac
     1861 cagttccgcg atggttttcg cgttcggcgt atcaaccagc gtcatttcct gggtagcagc
     1921 agcgcgcggt tctttcggcg cgatagcttc tgccagttca atgttcgctg catagtcaga
     1981 ggtgtcggag aagaccacat cgtcttcacc gctctgcgcc agcacctgga attcgtgaga
     2041 ggcgctgccg ccgatagaac cggtgtcggc ttgtacggcg cggaaatcca gccccatgcg
     2101 gctgaagatt ttgctgtagg ccgcatacat tgcatcgtag gtttcctgca gggattcctg
     2161 agaagtatgg aaagagtaag catctttcat caggaattcg cgggaacgca tgacgccgaa
     2221 acgcggacgc acttcgtcgc ggaacttggt ctggatctga tagaagttca gcggcagctg
     2281 tttgtaagag ctaagctcgt tacgaatcag gtcagtgata acttcttcat gagttgggcc
     2341 gagtacgaac ggacgctcgc cacggtcaac aaaacgcagc agttccggac cgtactgttc
     2401 ccaacgacca ctctcttgcc acaaatcagc tggctgaacc accggcatcg acacctcgat
     2461 cgcaccggcg ttgttcatct cttcacgcac gatgttttcg acttttttca gaacgcgcac
     2521 gccggtcggc agccaggtat ataacccgga ggccagcttg cggatcatcc cggcgcgcag
     2581 catcagctga tggctgatca cctcggcgtc ggcaggtgtc tccttgagag tggagagcag
     2641 gtattggcta gtacgcatgt tgttacggtt ccagttggaa ggtagaacag gctcaaggcg
     2701 agcctgggac aaaaaaaagt gatttagttt accagtgcaa aagaaatgtc aaaagagaag
     2761 ggcgtgaatt taacgcggtt ccagcgcaaa gacttcaaaa cctgcgtcgg tgacgcgcca
     2821 gcgaacgtta aaatcatgta gccagacggc ataggttttg cccgtttcct cacctttacg
     2881 ataggccggg cgtgggtcct gcgccagtac ttcgcggata aacagcgtta actgcggata
     2941 acgcttctcc agcgtcaaaa gctgcttttc gacctctgcg gtaaaactca ccgccatctc
     3001 tgccgctggc gcactttgcg catagctggc actggcatcg ggaagcgatt cggcaaaggg
     3061 gagatacggt ttgatatcca ctaccggcgt accatcgacc agatccagac tgccgagctt
     3121 cagaatcacg ctgtctttat ggcaaacaac ctctttcagc tctaccagcg acataccaat
     3181 tgggttaggg cggaaagtag agcgtgttgc gaaaaccccc attctggcgt taccgccgag
     3241 gcgcggtgga cgcacagtcg gacgccagcc gccttccatc gtttgatgaa agacgaaaag
     3301 gatccataaa tggctgaacg cttccaggcc gcgaacggcg tcggcctggt tgtagggagc
     3361 aatgagatgc agttcgccgt tggcgctttt taccagacct ggctggcgcg gaacggcgaa
     3421 tttttcttta tagggcgagc gaataacgcc tatttgctcg aactggaaac tgctcatttc
     3481 gccgtaatgt taagcgcaga gccgatacat acagcctgac gatagcagcc tggcgtaccg
     3541 ctggtgactt cgcagctatg cagtaatacc gcattggcct tcattttcga ggcgttgatt
     3601 tgcatccgct tacgcgcggt tggaatgctc ggcggagagt cctgattaga ggcctggcaa
     3661 gagtcgccac tgacttcacc gagatcgcgg aacggtttgc cgactaattc ttctgcattg
     3721 gtataaattc ggaccggcgt ggcgcgcggc gctttcggtt ttgcaggctc cgctttcggc
     3781 tggggtgcag tgctttgaac gggttcgaca ggggatctgc ttaacatgga acagccgctt
     3841 agcatgagtg ctactaaaca gatcggtaaa gcacgcatag tatttcctca atgtatgatc
     3901 aaaacgtcaa tattgaatca ggagcttgta aaaatgacaa gacgggcaag cgcccgtcct
     3961 gaatgatatt acaaattgtg gaaacagcct aaaaattacc agcctttaac agcgccgccg
     4021 ttaaacactt tgtttgctgc ttcgtaaact tcatcagact gataagcctg gacgaatttc
     4081 ttcacgttct cggcgtcttt gttatcttca cgcgttacga tcaggtttac gtacggggag
     4141 tctttatctt cgacaaagat accgtctttc gctggagtca ggccaatctg gctggcatag
     4201 gtggtattga taactgccag agcgatttgc gcgtcgtcca gagatcgagg cagctgcggt
     4261 gcttccagct caacaatttt cagatttttt gggttctcaa caacatcaag aacggtcggc
     4321 agcaggccaa cgccatcttt cagtttgatc aagcccactt tttgcagcag cagcagtgaa
     4381 cgaccaagat tagttggatc gtttggcacg gcaacctgcg aaccatcctg cagttcatcc
     4441 agtgatttga ttttcttgga gtaaccagca atcggataaa caaaagtgtt gcctactgcg
     4501 accagtttgt agccacgatc tttcagttgc tgatcaaggt acggtttatg ctggaaggcg
     4561 ttggcgtcga tatcgccttt gctcaatgct tcgtttggca gaacatagtc gttgaaggtt
     4621 accagctcaa cgtccaggcc atatttgtct ttcgcaactt tctgcgcgac ttctgctacc
     4681 tgctgttcgg caccaacaat cacgccgact ttaatgtggt ttggatcttt ttcatcctga
     4741 ccgcagccta ccagtgccag tgatccaatc agggctccca ctgccgcaaa ggttttgaat
     4801 ttgaacgcca taccttattc cttcttcaat tatttatgtt gtgttgaacg ttacttgcga
     4861 gtgacagccc ggacgatgcg gtcgcctgcg aactgaatta aataaaccag aatgaccagc
     4921 aataccagta ccgtattcat caccgtcgcg ttatagccga tgtagccata ctgatagcca
     4981 atctgaccta aaccaccggc accgactgca ccacccatcg cggaataacc gaccagggta
     5041 atcagggtga tagttgccgc attcaccaga cccggcaatg cttccggtaa cagcacttta
     5101 cggacgatct gcatcggcgt ggcacccatt gcacgggaag cttcaattaa cccggttggg
     5161 atctccagca gagcgttctc gaccatacgg gcaataaacg gtgctgcacc aacggttaac
     5221 ggaacaatcg ctgcctgcaa accaatcgat gtaccgacaa taacgcgggt aaacggaatc
     5281 atccatacca gcaagataat gaacgggatg gaacggaaaa tgttcacaat cgcagaaacg
     5341 gtacgataca gcttcgcgtt agcaataatt tgccccgggc gcgtaacata aagcaggacg
     5401 ccgaccggca gaccaatcac aaagccaaaa aatccggata cgaaggtcat tgccagcgtt
     5461 tcccatacgc cacgaaccag caaccacatc atcggctcag acataaccca gtacctctac
     5521 ttttacatgg tgttcctgca gccaggcaat ggcggcttgc gtatcttgtt gtgtgccgtg
     5581 catttcagtc agcatgatgc cgaacttaac gccaccggcg taatccatct gcgcgctaat
     5641 aatgttgttg ttgacgttga aacgacgcgc ggtttcagaa agcagcgggg catcgaccga
     5701 ttgaccggta aactccaggc gcaacatcgg cacgcagtca gtaaatggct ccgcttgcag
     5761 acgttcctgg taatcttccg ggatatccag atgcagggtc gactgaataa acttctgcgc
     5821 cagcggcgtt ttcggatgcg agaacacttc acttaccgtg tcctgctcga tcagttcgcc
     5881 attgctgatg accgccacgc aatcacaaat acgcttcaca acgtccattt cgtgggtgat
     5941 caacagaatc gtcaaaccca gacggcggtt aatgtctttc agcagttcga gaatagaacg
     6001 tgtcgttgcc gggtccagcg cgctggtggc ttcatcacac agcaatactt tgggattgct
     6061 ggctaatgca cgggcaatcg ccacacgttg tttctgccca ccggaaagat tcgacgggta
     6121 gctatcatgc ttatcgccaa gaccaaccaa tgacagcaat tccgtcacgc gacgtttgat
     6181 ctcgtctttc ggtgtgttgt ccagctccag cggcagagcc acgttgccaa aaacagtacg
     6241 cgaagagagc aggttaaaat gctggaaaat cataccaatc tggcggcgag ctttggtcaa
     6301 ctcggattct gacagcgtgg tcagttcctg gccatcgacc agcacgctac cctcggttgg
     6361 gcgctccagc aggtttacac aacgtataag cgtactctta cccgcgcctg aggcaccgat
     6421 aacgccataa atttgtccag ctggcacatg caggctgacg ttgttcaacg cctggatggt
     6481 gcgggtgccc tggtggaaca ctttggtgat attcgaaagt tttatcattg attatttatt
     6541 atcgtcatta agttagtcgt ggcatctcga atgcctgaaa cgggcaacgc cgtcaatgaa
     6601 atggatgtta aggcatccag acgtctaaat caatcaggtt tatgcgaaga gcactttctt
     6661 gcaggtcgaa acatgcgata ctagcgtccc atgccttatt aaggagctat aaaaggtggc
     6721 gaagagcgta cccgcaattt ttcttgaccg tgatggcacc attaatgtcg atcacggcta
     6781 tgtccatgag atcgacaact ttgaatttat cgacggtgtt attgacgcca tgcgcgagct
     6841 aaaaaaaatg ggctttgcgc tggtggtagt aaccaaccag tctggcattg ctcgcggtaa
     6901 atttaccgaa gcacagtttg aaacgctgac cgagtggatg gactggtcgc tggcggaccg
     6961 agatgtcgat ctggatggta tctattattg cccgcatcat ccgcagggta gtgttgaaga
     7021 gtttcgccag gtctgcgatt gccgcaaacc acatccgggg atgcttttgt cagcacgcga
     7081 ttatttgcat attgatatgg ccgcttctta tatggtgggc gataaattag aagatatgca
     7141 ggcagcggtt gcggcgaacg tgggaacaaa agtgctggtg cgtacgggta aacctattac
     7201 acctgaagca gaaaacgcgg cagattgggt gttaaatagc ctggcagacc tgccgcaagc
     7261 gataaaaaag cagcaaaaac cggcacaatg attaaaagat gagcggttga aataaaaatg
     7321 catttttccg cttgtcttcc tgagccgact ccctataatg cgcctccatc gacacggcgg
     7381 atgtgaatca cttcacacaa acagccggtt cggttgaaga g
//
LOCUS       DAZGOB010000185         7397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGOB010000185 DAZGOB010000000
VERSION     DAZGOB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.185"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..278
                     /locus_tag="ACHLIF_004633"
     CDS             <1..278
                     /locus_tag="ACHLIF_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFO9608489.1"
                     /translation="SPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEVWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            278..5548
                     /gene="traI"
                     /locus_tag="ACHLIF_004634"
     CDS             278..5548
                     /gene="traI"
                     /locus_tag="ACHLIF_004634"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO9608490.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMIAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTVGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAREESVAQVSGVREQAILTQAIRSELKTQGVL
                     GQREVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGRIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDAPRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTDLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     TGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTELAVRDIAGQERERAVTSEREAALPESVLREPQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5568..6314
                     /gene="traX"
                     /locus_tag="ACHLIF_004635"
     CDS             5568..6314
                     /gene="traX"
                     /locus_tag="ACHLIF_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO9608491.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVVAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6369..6929
                     /locus_tag="ACHLIF_004636"
     CDS             6369..6929
                     /locus_tag="ACHLIF_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO9608492.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGFFDGDTPRLLACGIRE
                     VLLEDVSRRNIPLSHKKLRRALKAVTRSESYLGAMKAGACRYDAEGYVTEHISQEEEA
                     YAAERLDKIRRQNRIKAELQAVLDEK"
BASE COUNT         1695 a         1854 c         2439 g         1409 t
ORIGIN      
        1 tgtctcctgc catcaacgat aagaagtcag attcaggtgt gaatgttccg gcagggggga
       61 tcgagcagga gctgaaaatg aaaccggaag aagagatgga acagcaactg ccacccggga
      121 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggtatggcaa caggaaaatc
      181 atccggacat ccagcagcag atgcagcgtc gtgaagaggt gaacattaat gtgcaccggg
      241 agcgtgggga ggatgttgag ccgggagatg atttctgatg atgagtatcg cgcaggtcag
      301 atcggccgga agtgccggta actattatac cgacaaggat aattactatg tactgggcag
      361 catgggagaa cgctgggccg gcaggggggc tgaacagttg ggactgcagg gcagtgtcga
      421 taaggatgtt tttacccgtc ttctggaggg aaggctgccg gacggagcgg atctaagccg
      481 catgcaggat ggcagtaata agcatcgtcc cggctacgac ctgaccttct ccgcccccaa
      541 aagtgtctcc atgatagcca tgctcggggg ggacaagcgt cttattgaag cacataacca
      601 ggccgtggac tttgctgttc gtcaggtgga ggcgctggcc tccacacggg tgatgacgga
      661 cggccagtca gaaacggtac tgaccggtaa tctggtgatg gcactgttta accacgacac
      721 cagtcgcgat caggaaccac agttacacac gcatgcggtg gtggctaatg tcacgcagca
      781 taacggtgag tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa
      841 tgtgtacgct aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt
      901 tgaggcgctg ggctatgaaa ccgaagtggt gggtaagcac ggtatgtggg agatgccggg
      961 cgtaccggtg gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga
     1021 cgcctcgctg aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcatgt
     1081 cgatcctgag atcaaaatgg ccgagtggat gcagacgctg aaggaaaccg gattcgacat
     1141 ccgggcatat cgtgacgcgg cggaccaacg ggcggagacc cgcacgcaga cacccggacc
     1201 cgcttcacag gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag
     1261 tgaacgcaaa gtgcagttca cgtacacgga cgtgctggcc aggacggtcg gcatactgcc
     1321 tccggaaaat ggtgtgatcg agcgggctcg tgccggtatc gatgaggcca tcagccgtga
     1381 gcagcttatc cccctcgatc gtgagaaggg gctgttcacg tccgggattc atgtgctcga
     1441 tgagctgtcc gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca
     1501 tccggagaaa agtgttcccc ggacggtcgg ttacagcgat gccgtcagtg ttctggcgca
     1561 ggatcgcccg tcgctggcca ttgtgtcagg gcagggcggt gcagccgggc agcgtgagcg
     1621 ggtggctgaa ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc
     1681 tgaccgtcgt tcgcagatga acctgaagca ggatgaacgg ctgtccggtg agctgataac
     1741 cggacgtcgt cagttgctgg agggcatggc cttcacaccg ggtagtaccg ttatcgttga
     1801 ccagggcgaa aaactctccc tgaaagagac gttaactttg ctggacggtg ccgcacgtca
     1861 taacgtacag gtcctgataa ccgacagcgg gcagcgaacc ggtaccggca gtgcactgat
     1921 ggccatgaaa gatgccgggg tgaacacata tcgctggcag ggcggggaac agcgaccggc
     1981 aaccatcatc agtgaaccgg atcgtaatgt ccgctatgcc cggctggccg gagattttgc
     2041 ggccagcgtg aaagccagag aagagagcgt ggcacaggtc agcggggtac gggaacaggc
     2101 catactgaca caggccattc gcagtgagct gaaaacacag ggcgtactcg ggcagaggga
     2161 ggtgaccatg acggcgttgt caccggtctg gctggacagc cggagccgtt atctgcggga
     2221 tatgtaccgt ccggggatgg tgatggagca gtggaacccg gagacacgca gtcatgaccg
     2281 ctatgttatc gaccgggtga cggcgcagag tcacagcctg accctgcggg atgcgcaggg
     2341 tgaaacgcag gtggtgcgta tttcctccct ggacagcagc tggtcgctgt tccggccgga
     2401 aaaaatgccg gtggcagacg gcgagcgact gagggtgaca gggagaattc ccggactccg
     2461 cgtctccggc ggtgaccgcc tgcaggtggc atccgtcagt gaagatgcga tgacggttgt
     2521 tgtgccgggg cgggctgaac cggcctccct gcctgtgagc gattcaccat tcacggcact
     2581 gaagctggag aacggctggg tggaaacgcc cgggcattcc gtcagcgaca gtgcgaaggt
     2641 ttttgcctcc gtcacacaga tggcaatgga caacgccacc ctgaacggtc tggcccgcag
     2701 cggtcgtgat gtccggctgt attcctctct ggatgccccc cgtactgcgg aaaaactcgc
     2761 ccgccatccg tcctttacgg tggtttctga gcagataaag gcgcgtgccg gtgagacatt
     2821 gctggaaaca gctatcagtc tgcagaaagc cgggcttcac acgccggcac agcaggctat
     2881 tcatctggcc cttcctgtgc tggaaagtaa aaacctggcc ttcagcatgg tggacctgct
     2941 gacagaggcg aagtcgtttg ctgcagaagg aaccagtttt actgacctgg gaggggaaat
     3001 caatgcgcag ataaaacgcg gtgatttact gtatgtggat gtggcaaaag gctatggcac
     3061 aggcctgctg gtttcccgtg cgtcgtatga ggctgaaaaa agcattcttc gccatattct
     3121 cgaaggtaag gaggcggtca cgccgctgat ggagagagta cctggcgaac tcatggagaa
     3181 actgacatca ggacagcgtg ccgccacccg catgatactg gaaacgtccg accgtttcac
     3241 ggtggtgcag ggctatgccg gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc
     3301 cgtgaacatg ctgccggaga gtgagcgtcc ccgcgtcgtg gggctgggtc ccacacaccg
     3361 tgcggtcggg gagatgcgca gcgccggcgt ggatgcgcag acactggcgt catttctgca
     3421 tgacacgcag ctgcagcagc gcagcggaga aacgccggat ttcagcaaca cgctgttcct
     3481 gctcgatgag agctcaatgg tgggcaatac cgacatggca cgggcatacg ccctgattgc
     3541 gaccggtggc ggtcgtgccg tggccagtgg tgacacggac cagttgcagg ccatcgcgcc
     3601 cggtcagcct ttccgtctcc agcagacgcg cagtgctgcc gatgtggtca tcatgaagga
     3661 gattgtgcgt cagacgccgg aactgcggga ggcggtatac agcctgatta accgggatgt
     3721 ggaaagggca ctgtccgggc ttgagagtgt gaaaccgtct caggttcccc gtcaggaggg
     3781 cgcatgggca ccggagcact ccgtgacgga gttcagtcac agccaggaag cgaaactggc
     3841 agaagcgcag cagaaggcga tgctgaaagg cgaggctttc ccggatatcc ccatgacact
     3901 gtatgaagcc attgtccgcg actataccgg caggacaccg gaagcacggg agcagacgct
     3961 gattgtcaca cacctgaatg aggaccggcg cgtactgaac agcatgattc atgatgcacg
     4021 ggaaaaggcc ggtgagctgg gcaaagagca ggtcatggtg cctgtcctga acacagcgaa
     4081 tatacgtgac ggagaactgc gtcgtctctc cacctgggag aataacccgg atgcccttgc
     4141 cctggtggat agtgtgtatc accggattgc cggtatcagc aaggatgacg ggctgataac
     4201 cctggaggat gcggaaggta acacgcggct gatttcgccc cgggaggcgg tggctgaagg
     4261 cgtcacactg tacaccccgg acaccatccg ggtgggaacc ggtgaccgga tgcgcttcac
     4321 gaagagtgac cgggagcgag gttatgtggc caacagcgtc tggacggtga cagcggtttc
     4381 cggtgacagt gtcacgctgt cggacggaca gcagacccgg gtgattcgcc caggtcagga
     4441 gcgggcagag caacatattg acctggctta tgccatcacc gcccacggtg cgcagggggc
     4501 aagtgaaacc tttgccatcg cgcttgaagg cacggaaggt aaccggaaac tgatggccgg
     4561 ctttgagtca gcctacgtgg ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga
     4621 taaccgtcag ggctggacgg atgccatcaa caatgccgta cagaaaggaa ctgcccacga
     4681 tgtgtttgag ccgaaaccgg accgggaggt catgaatgca gagcggctgt tcagtacggc
     4741 gcgggagctg cgggacgtgg cggcaggccg tgccgttctt cgtcaggcgg ggctggccgg
     4801 gggagacagt cctgcacggt ttattgctcc gggacgtaaa tatccacagc cgtatgtggc
     4861 actgccggcg tttgaccgta acggcaagtc tgccggtatc tggctgaacc cgctgaccac
     4921 ggatgacgga aacgggctgc ggggattcag tggtgaaggc cgtgtgaaag gcagcgggga
     4981 tgcgcagttc gtggccctgc agggcagccg taacggcgag agcctgctgg ctgataatat
     5041 gcaggatggt gtccggattg cccgtgataa ccccgacagc ggtgtggtgg tgagaatcgc
     5101 cggtgaaggt cgtccgtgga atcccggtgc catcaccggt ggccgcgtgt ggggggatat
     5161 cccggacaac agcgtccagc cgggagccgg aaatggcgag ccggtcacgg cagaggtact
     5221 ggcacagcgg caggctgaag aggccatccg ccgtgaaacg gaacgccgcg cagatgaaat
     5281 tgtccgtaaa atggcagaga acaaacctga cttgcctgac ggcaaaacag agctggctgt
     5341 cagggatatt gccgggcagg agcgtgaacg ggctgtcact tctgaacggg aagccgcgct
     5401 gccggagagt gtactgcgtg aaccacaacg ggagcgggaa gcggtccggg aggttgcccg
     5461 ggaaaatctg ctgcaggagc gactgcagca gatggagcgg gatatggttc gtgacctgca
     5521 gaaagagaaa accctgggcg gagactgaca caggaagata aacgctgatg acaacggata
     5581 acacgaacac aacacgtaat gattcactgg ctgcccggac cgatacctgg ttgcagtcgt
     5641 tgctggtctg gtcacccgga cagcgggata tcatcaaaac ggtggcactg gtgctgatgg
     5701 tactggacca tgcaaaccgc attctgcatc tggaccagac gtggatgttt ctggccgggc
     5761 gtggggcctt tccgctgttt gccctggtgt gggggctgaa tctgtcccgg catgcacata
     5821 tccggcaacc ggccattaac cggctgtggg gatggggaat tattgcccag ttcgcatatt
     5881 atctggcagg ctttccctgg tatgagggga atatcctgtt tgcctttgca gtggtggcac
     5941 aggtgctgac gtggtgtgaa acgcgcagtg ggtggcgtac agcagcggcc attctcctga
     6001 tggccctgtg ggggcctttg tccggcacca gttacggcat tgccgggctg ctgatgctgg
     6061 cagtcagcca ccggctgtac cgggcagaag acagagcgga acgtctggcg ctggtcgcct
     6121 gcctgctggc tgttattccg gcgcttaacc ttgccaccag tgatgccgcg gcggtggccg
     6181 gtcttgtgat gacggtgctg accgttggtc tggtgtcgtg tgcaggaaaa tcattacccc
     6241 gtttctggcc cggagacttt ttcccgacgt tctatgcctg tcatctggct gtgctgggcg
     6301 ttctggcgct gtgactggtg tggtatcttt ggccgcaaga ggatgattcg tcagaggcag
     6361 aacacagcat gacagagcag aagcgaccgg tactgacact gaagcggaaa acagaagggg
     6421 agacgcctgt ccgcagccgg aaaactatca tcaatgtcac cacgccacca aagtggaagg
     6481 tgaaaaagca gaagctggcc gagaaagcag cccgggaagc agagctggcg gcaaaaaaag
     6541 cgcaggccag acaggcgctg tccatttatc tgaacctgcc cacactggat gaggccgtga
     6601 ataccctgaa gccctggtgg ccggggtttt ttgacggtga caccccccgg cttctggcct
     6661 gcggtatccg ggaggtgtta ctggaagatg tatcgcggcg gaatatcccg ctttcgcata
     6721 aaaaactgcg cagggcgctg aaggctgtta cccgttcaga aagctatctt ggcgcaatga
     6781 aggccggtgc ctgccgctat gacgcggaag ggtatgtgac ggaacatatt tctcaggagg
     6841 aggaagcgta tgcggcagag cgtctggata aaatccgccg ccagaaccgg ataaaggcag
     6901 aacttcaggc cgtgcttgat gagaaataaa aaggcctccc cgaaagagtg aggaggctga
     6961 aacaataagg cttaaaggga aataacttcc gtaccagaaa ggaagtcagt ccactgaatt
     7021 atttaacaca ataatactga tggcttaaag ggggaaaatc aaagattaac cgctcacgaa
     7081 aggtctggcc tggcgctggc agcgtgtctg aagacgctgg ggcatgggca ccttcagatt
     7141 atgtggtttc tctggcagac tgatagctgt ttccccggca aagagcggaa tacagctacc
     7201 agtgctgccg gagtaaggtg taacgaaaaa aacgccttcg ttactccagg agtcagaagg
     7261 ctgaataatg tatgagattt tagtcttata tctggtctgg cagtatgacc aggtgatttc
     7321 tattatcaca gactcgtttt atatataatg atatattatt atatttgtga aatttgtcac
     7381 aaaatattaa atctggt
//
LOCUS       DAZGOB010000186         7329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGOB010000186 DAZGOB010000000
VERSION     DAZGOB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.186"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(8..1018)
                     /gene="pyrD"
                     /locus_tag="ACHLIF_004637"
     CDS             complement(8..1018)
                     /gene="pyrD"
                     /locus_tag="ACHLIF_004637"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO9608493.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(1129..1866)
                     /locus_tag="ACHLIF_004638"
     CDS             complement(1129..1866)
                     /locus_tag="ACHLIF_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO9608494.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1832..2347)
                     /locus_tag="ACHLIF_004639"
     CDS             complement(1832..2347)
                     /locus_tag="ACHLIF_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9608495.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2355..2897)
                     /locus_tag="ACHLIF_004640"
     CDS             complement(2355..2897)
                     /locus_tag="ACHLIF_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9608496.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2909..3979)
                     /locus_tag="ACHLIF_004641"
     CDS             complement(2909..3979)
                     /locus_tag="ACHLIF_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9608497.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3970..6570)
                     /locus_tag="ACHLIF_004642"
     CDS             complement(3970..6570)
                     /locus_tag="ACHLIF_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO9608498.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(6595..7296)
                     /locus_tag="ACHLIF_004643"
     CDS             complement(6595..7296)
                     /locus_tag="ACHLIF_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9608499.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
BASE COUNT         1861 a         1877 c         1636 g         1955 t
ORIGIN      
        1 gaaataatta gatatgggta acgatttctt taatcagcgg cggaccttta aaaataaaac
       61 cagaataaat ttgcaccagt gaggcacccg cagcaatctt ttcacgcgca gcgataaccg
      121 agtcgatgcc gccaacaccg atgatcggta agcgaccgtt taattccagt gacaagcggc
      181 gaataatttc ggtgcttttt aactgaagcg gacgaccact taagccaccg gtttgatcgc
      241 aatttttcat tccctgaaca agagaacgat cgagtgtggt attggttgca ataacgccat
      301 caatattatg gcgaactaaa ctatcggcaa cctggatcaa ttcttcttca gaaagatccg
      361 gcgcgatctt cactgcgatc ggcacatatt tatggtgcat cgcttgcaaa tcattttgct
      421 tatttttaat cgcggttaag agatcatcca gcgcttcacc atattgcagc gtgcgtaatc
      481 ctggggtatt cggcgatgaa atattgatgg cgatatatcc cgcataggca tagatttttt
      541 ccatacaaat cagatagtca tctttgccct gctccactgg cgtatcttta tttttgccga
      601 tgttaatacc caggacgccg tcataatggg ccttttttac gttctctacg aggttatcaa
      661 cgccaagatt attaaagccc atacggttga tcaaaccttc ggcatctacc agacgaaaga
      721 gacgcggctt gtcattacct ggctgtggac gtggcgtgac ggtaccgatc tcgatcgatc
      781 caaatcccat cgcgcctaac gcgtcaatgc actccccgtc tttatcaaga ccggctgcca
      841 gaccaagcgg atttttaaac gtcaggccca tgcagttaac aggtttcgca ggcactttct
      901 gccgcaccag tgcttcaaac ggcgttcctg taatacggcg taattgctga aaagtaaact
      961 catgagcgcg ctctggatcg agctggaaaa gggctttacg aacgaagggg tagtacatga
     1021 actctcctgg attcccggtg tgcaaaccgg gggcgtatta tgtgcgagtt cggaccaaaa
     1081 gggaattgac ctgcggcaaa aaaaacgcaa acgttttccg aacctgtttc agggcagatt
     1141 ttgctccttc tttgcactca acccgccgta atcatttatc gcccgccagg cgacatgaca
     1201 cggtgcgatt gcctgacacc agcttgtctg acgctggctt ttaggcggca caactcccgc
     1261 attacttaac gctttaccat ttacgctcaa ctgaatcagg ttaatgtagt aaggcgttgg
     1321 gttagtgagt tgtacaccct gcgaattccg tgtccagcgt aattgttgat aagcttccag
     1381 cgggtcgccc ggcaaacctt cgggtcgcca gaacagttta aataccgagc gcatcgccac
     1441 ttttacgctc tgattttcaa ctttgccgga tggcacgctg gcaatgctta actcaaatag
     1501 cgtttcgcga tccacaggca agatgtcgct ttccgttctc agcaagcgca acgtgcctgt
     1561 agtaccgggc ttcaggagaa taagtggcgg agcggccagt aatggtgctg gcactgtcga
     1621 cgcttccccc ccggcccaac gcgttgggcg gttgatttta ctgttaataa gccaggattc
     1681 ctgactggta ttagtcagta aaatagatat cgattctctg tctgccggaa agataaatcg
     1741 cgttccgccg accaccactc ctgcccgcaa ttcacccgcc accagcagcg ataaaacggc
     1801 gaaaatcaag agcgccaatc tgttccacgt attagtcata atgaagtaca aatgtcgcgc
     1861 tggcattcac tttacccgac tgcacatcgc tattgacagg tcgcaattgc gcataaaaag
     1921 ttaatggcac cttaccgcta tgtaggctgt agtctttact cgcctggtta agcggaatca
     1981 atatcttcgc atcatccaga atcgcaattg ccagcccgct ggctgcatcc ataccacttt
     2041 caatggacag catttcgctc tgcactccgt cgggggttcc tgaaaaggtg acgtcaaccg
     2101 tgctgacatc tgttccgcaa tcctgcaaat taatgatgaa agatttctcc gggccataat
     2161 tcccgtgaga aaaacgtgtc agcgagacat cgcctaactg cacattgatt tgcttgctat
     2221 cctgtgccag cacgcatgta ttacgacgaa acgatccgct aatgttgacc gtgccatcat
     2281 gagcaaataa ctcactactc cccgttaatc ccagtaagca cagaatccag attatccgtt
     2341 tcaacatcac tccttcactg gtaaaccaga tcaaaataga gaaccgccgt cgcattaccg
     2401 cccgttgctc ccgcctttgt ggacttataa cgaagttgat atttgagtgt gttatccccg
     2461 gcttttaagg gcgtaagagg ctggacctga ttgagcggga tttcttgctg actttgcgca
     2521 tcaagaattt gcacggcaat ccccgttgcc ataccaccgc ttccgcctgt gtcagttagt
     2581 ttaagcagtg tcggcgcaag ggtgtcagcc tcgccgctaa aaaggacgtc cgcgccagta
     2641 ataccagcgg cacaaccggt gatatcgata ttcaaatcag ctgcagtgct tacggtattc
     2701 gcggcctgaa aatcactgat attaaaatcg ccaatatgaa tattttgtaa tgcacttttg
     2761 gtggcgacat cacagccctg atcgaccaca ttcccataaa tatgcatact gacttgttca
     2821 ggcccagcca tcccgacatg gatgttaaga aggctaaaga acaaaataac gtactgatat
     2881 atcgtttttt tcttcatacg ttcacgcgtt aatcaaaatt tattctcacc gttgccgtgg
     2941 cttcaaatcg cccggtttct ggtggtacgc ctgtcgtgct gactggatag acattcattc
     3001 gcgcgatact gtctgcatca tcaggaatag agagcggtag tgtcccacca gcaggggaaa
     3061 taatgttgtt ctggctatcg gtcaccacca cgcccacgcc aggacgtgac gtcttaatcg
     3121 cctggggata gctggggtct gaggttgccg tcagactcag gttgacgtta actaaccctt
     3181 cgtttcccgt gttacacctg actggaattg cgagttctgc cgttctggcc ccaaggggtt
     3241 tatgccctac aacacgaaag ttagccgcaa atacgtcgcc cagatcgacc tcgatagtct
     3301 ctccggcatt gacggaacat gagccaagtg aattgatcgt accagacagg taatacacta
     3361 aaaccggtgt acctgtagtc gtgcaggaat cacctgcggg tatgttgtag cattcaaata
     3421 atcgcgccag cgccatagga ggaataacga ccgaaccaac gaacggctgc aatattttca
     3481 gggaaaagct accgctatta ccgctagccc cctggccacc taaacgttga ccgccgggat
     3541 cgcaaatatg ccatcgtgta tctgcccctg tgcccacgtt ttcgaaagga accgcagtag
     3601 gagaactgcc tttaacctgc aataaaactt tggcggcaag atagtcatta attttgtacc
     3661 agttgtcgcc taaatagact aacggtgaat ccgcggcgaa tgcccatcgg ccacttctga
     3721 cattgtcttt atcgcaatca caggaaacat aataatcatt cgattcgttc cactgataaa
     3781 atttgtcttt aactactcca gccgtatttt cttcttcctc cagggtttta acgccaaaat
     3841 tgacgccaat atacggattc ccctgagtgg tatgacactg cccgctataa gcagcagttt
     3901 gagcgcacca tagcattaag acggcggcaa aaaatagtcg tagtaataac acgcattttt
     3961 ctccaaagat tatctgcata tggcatccat tcgcgtaatc gcagagttaa tttgttgttc
     4021 ggtaagctgg taactgatat gacaaatttg atcgggagct tcgccccagc gtacagttat
     4081 cgctccctga gcgggcaaac cggacatata gagcgagcca tcatcatcaa taatgccgct
     4141 attagtctgt atgccgtcca gcgtcgctat cgcgccaaaa cgcagcggta taccctgctt
     4201 tgatgttttc attaataccc ttgctcccgc atgggcattg aactccgcca gaaccaacgc
     4261 ccctttggtg ggaaccacgt tgactactgc attttcaaga tccacgttac gttttaacga
     4321 tgccgcatca agtgcgattc gattttcccg ataagaagtg gcatagggga caacggtata
     4381 tccgcgccag tcagttttca gcccggtgct gttcgccaca cgagtattct ctgcgcctgg
     4441 tgctgcaatc agcacgttag tttcccccag cgattgcccc agggtaatgc cctgtgaatg
     4501 ggcaactaaa ctgcctgaaa gagcgtagtt cagttgttgt tgactgccgt tatcgctgta
     4561 gttgtagccc actcgggcat ctgcaaacgc ccctttgtag tccatgctgg cgctaccatt
     4621 agccgttttc ccctcgctgt tatatccctg ttgcacgctg tagctcaggt taccgtcatc
     4681 gagtagtgtc tccgttaaac ccaggttttg tgtaatgtgc ccttcgttat cgatactggt
     4741 gttagaggtc atccaggcgt aatttttttt cctggtatag ctatcattac ttctggggag
     4801 caacagattg agcggcaggg aaaaatttag tgcaaacacc tgatcagcat caggttgacc
     4861 acgggactta ctgtagttcc aggaaacccc cagcgagaga tctttaattt gcgtgttgta
     4921 gccaaactgt aatagccgat cctgttgatc ggtatgccag taagtttgct ggctaccact
     4981 taaataaaac gaaccgtact cgcctaattg ctgggagata ttgacctgca gtttgccacg
     5041 tttggtgtaa aacaaattgt aataacgcga ccacatcggc gtatcttcat catcaccgtc
     5101 attaaattcg tagccatcca tatgtttata catggtgtcg gaaagggtgt agaaacccga
     5161 cgtcgaatag cgatagccca tcaattgaaa attagtgccc aattggttga gcgttttggc
     5221 atacagaaaa cgatatgact gacctttatg ttcgctttgg tctgccagcg tactcttcgc
     5281 ctgggtcgca tcgaacgaaa ttgctccgaa atcgccaagg ttaaatccca gaccaaacat
     5341 ggcggcacgg taatattcag catattgtcc accaccatac catgtcgttc cccacggtcc
     5401 gccccactgc aacgtggcct gggcaaattt gctctcctgc tgttcattac tattggttct
     5461 gtatttcgcc agtgtcaccg cgtatttgat tcgcccctga cgctggagta atggcacgct
     5521 ggaaaacggt acgctgtagc tattgacgct gccgtccgct tctttgattt caactaacaa
     5581 atcaccgctc gacgacgtgg aatagagatc actaatttca aaagcaccag gcgatacata
     5641 agtttggtaa atggtgtaac cattttgctt aatcgttatc tgggcattgc ttttggcgat
     5701 accacgcacc acaggcgcaa accctttcag gctgtctggc agcatgttgt catctgacgc
     5761 cagctgcaca ccacgaaagc tgacactatc gaaaaaatcg cctgcagtat aatcatcacc
     5821 tacggtcagt tcgcctttta gtggaataac cgcccgctgc aaatagctgc tgagattctt
     5881 ccattctgcg gtttggccac tactgcgact ccatgttgaa ttgttgcgca atctccatgg
     5941 gcctaaatta acgccactgt ttaaattcag aaaatagctg tcgccagaat cactgtctgc
     6001 gctgctatga atactgttag ccccgctaaa cgaatatccc agtaatagcg cgttgatccc
     6061 ttcatcccaa cgttcagggg ggacataacc acgggcatta cttttgatgg cgatttgcgg
     6121 tactgaaatt tccagacgga gtttattcac attaaacgtt acgctggcat ccggaataat
     6181 ctccgccaat ggcacacacg cttgtttgtc cagttgttta aaagccggaa acgcatcagt
     6241 attaacaccc attgcatcca ggctttcagt cgtcaaacag gcggcaatcc ccttctctgg
     6301 cgttatctca acaaaattga cgttgcgcgt atctacaatt gtctgattca gaacaatatc
     6361 gacacgataa acccctggcg ttattttttg tcctttttcg aaacgagaca aatcggccac
     6421 cattgaggga tcatctttga aaaaagcagg atcgaaccag gtttcagctt gtgcattgaa
     6481 cgctgccgac acaaacacca ccagcccacc aataaacgat ggcactccac cagagcttaa
     6541 caggctgtgt tgtcgatgag ttctatacat ctcgctctct taactgaaga cggcttacga
     6601 tttacagttg agttgcggcg tttccgcccc gtagtcattg atggttcgat acgttacgct
     6661 gctatttttg gccccctttg gcagagaaaa ttggtattta tcaaaggggg aaatcatttg
     6721 atttttaact acttttccac ctattttcag gtctgtcagg gtaatgaaat aaggtgttgg
     6781 gttactgacc gtaaccatgt cagctgacga agtgcaacgc agtttcgccg gagcctcgcc
     6841 taagggatac gctaaacctt ttggtcgcca gaaaagtttg atgacgcttt gtgtggcaat
     6901 cttcagggta tttccctctt cccgcttcgt tgttgaaggg actgcccgta cgctagtgaa
     6961 gaaaagggtt tctctgtctt tcgccaacgg cgcaccaatg tacataatgc gtaacagatt
     7021 ctcattagcc ggattcagca caaatagcgg tggtgtaata ataaaatctg ctgatttttt
     7081 attatctggg gtcatcaccc atgactgaat aagataagga acatccggac tggtgttacg
     7141 tacacccagt gaaatttgct cttcaccttc tgaataaaca agacgtgttg ctgcaagtcc
     7201 aatcccgcct ttacctgccg cccatgcgga tgagcagctg agtaatattg ccgttaaact
     7261 tacggtcaca attccctttg ttatgcaagt tttcataaat agtcactctt aaaaaatatg
     7321 aatatcccc
//
LOCUS       DAZGOB010000187         7267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGOB010000187 DAZGOB010000000
VERSION     DAZGOB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.187"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1176
                     /gene="tssI"
                     /locus_tag="ACHLIF_004644"
     CDS             <1..1176
                     /gene="tssI"
                     /locus_tag="ACHLIF_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO9608500.1"
                     /translation="QEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQMDGWRNNAET
                     ARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGTALENH
                     FAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVRFNWDRYNPADQ
                     DSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSL
                     PGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHR
                     ETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGSVHVDAVNNGG
                     EGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
     gene            1204..1665
                     /locus_tag="ACHLIF_004645"
     CDS             1204..1665
                     /locus_tag="ACHLIF_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO9608501.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1685..6484
                     /locus_tag="ACHLIF_004646"
     CDS             1685..6484
                     /locus_tag="ACHLIF_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HFO9608502.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEIPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTESNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRSEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPDDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDAQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGTHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAALGQLSRYTAADGAEWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAETGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEETTWGGLLPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRVRQAHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGEWDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLQGGEQTYRYVPNPLGYIDPLGLAKTSVPAEKISLSDKARDLFRQGK
                     VREALDVHYEDLVRRKLGGISQEIAGREYDVVTDKIIAQVKRTYSSIDNPKNFLSKST
                     RTQIKKTIELAEEQGKEAQFWFKYGVSPKVREYIESKGGKVILGMGN"
     gene            6486..6851
                     /locus_tag="ACHLIF_004647"
     CDS             6486..6851
                     /locus_tag="ACHLIF_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004014665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608503.1"
                     /translation="MSRYILIRGWFECDFEQVKKIKEANEEFFKHSEKYLLPENTKRL
                     YLNGWCYPEYPINWVSVISLGVNINQIAFDYIKDMIVNVSKTERLDGYFRVNDDEDNL
                     FLEWIVKDGYLEEIILSGS"
BASE COUNT         1853 a         1849 c         2191 g         1374 t
ORIGIN      
        1 caggaaggcc agcaccagga ctaccagcgc acgcagtatg aagtgtatga ctaccccgga
       61 cgtttcaaga gcgcccacgg gcagaacttt gcccgctggc agatggacgg ctggcgaaac
      121 aacgcagaaa ccgcgcgggg aatgagccgc tcgccggaga tatggccggg acggcgaatt
      181 gtgctgacgg ggcatccgca ggcgaaccta aaccgggaat ggcaggtggt ggcaagtgaa
      241 ctgcacggcg aacagccaca ggcggtgcca ggacggcagg gagcggggac ggcgctggag
      301 aaccattttg cggtgatccc ggcagacaga acatggcgac cacagccgtt gctgaaaccg
      361 ctggtcgacg gcccgcagag cgctgtcgtg acaggaccgg caggcgagga aatcttctgc
      421 gacgaacatg gtcgcgtgcg ggtgaggttc aactgggacc gttataaccc ggcagaccaa
      481 gacagttcgt gctggatccg tgtggcacag gcgtgggcag gcaccggttt tggccacctg
      541 gcgataccgc gtgtgggtca ggaggtgatt gtagacttcc tcaacggcga tccggaccag
      601 ccgatcatta tggggcgcac ctaccaccag gaaaaccgca cccccggcag cctgccggga
      661 acaaagacgc agatgaccat ccgctccaaa acgtacatgg gcagcggatt taatgagctg
      721 aagtttgatg atgcgacagg gagagaacag gtctacatcc acgcgcagaa gaacatggat
      781 accgaagtgc tcaacgaccg taccaccacc gtaaaacacg atcaccgcga aaccgtaaaa
      841 aatgaccaga cggtcacgat ccaggaaggt aaccgccttc ttacggtgga aaaaggccac
      901 aagatcaccg gagtactgaa agggtcttta tctgaggatg tctttcagga cagaggcacg
      961 attgccggtt cggtgcatgt tgacgctgta aacaatggtg gcgaaggcga cggtatacag
     1021 gcttatacgg cgattaagga aattttgctg gctgtggagg aaagcaaaat tgcgctgacg
     1081 ccggatggca ttcagctaca ggtcggggaa tcgacggtaa tcaggctgtc gaaggatggc
     1141 atcaccatcg tgggcggttc tgttttcatc aactgattca ctcagattat cagggagtat
     1201 tttatgcagt tcacttttaa tgaaggccac attcaactgc cgtctcaatg gcaggatcag
     1261 tcaatgcagg tcctggtgtc cacagataac agtggcatca acctggtcat tacccgcgaa
     1321 gccgttccac agggaaccct gacaccagag ctgtaccagg aaacgctggc gctgtaccag
     1381 ggcaaacttg atgggtacac cgaacatgcc tgccgggaaa tcactctggc agaggccccg
     1441 gcctggctgc tggattattc ctggaacggt ccggaagatg aggggaatca ggggcggatt
     1501 agccagatag ccgtattcca gcggcgtgga gatacgctgc tgaccttcac tttcagcacc
     1561 tccttatccc tgaagaacag tcaaaagacg atgctgctgg aggtgataaa gagcttcacc
     1621 ccgttgccac cggagaatga catccaaaag gaccagccgc gctgacataa acgagggata
     1681 acacatgagt gaaggaccag gcgggccaca gggagcgacc gcaggcggta cgctggcaat
     1741 gcgaatgctg tcacagcagg cgatggtcgc cagccagatg aaacgggcag ccaacgacaa
     1801 agccattgca cagatgctgg cagcaaagaa gtccggccca cctgccgcca ggctgggcga
     1861 tgaaattcag cataaaagtt ttctgggggc actggcaggg gccgtgctgg gggcgatagt
     1921 gaccatcgca gaaggttgcc tgattatggc cgcctgcgcc accggccctt atgcgctggt
     1981 tctggtgcct gcgctgatgt atgccagcta taaggcgagt gattatgtgg aggagaaaca
     2041 gaaccagctt gaatcatgga taaacagctt ttgtgacacg gacggcgcca tcaataccgg
     2101 ttctgaaaat gtaaacatta acggaaagcc cgctgcaagg gccgccgtca cccttccccc
     2161 tcctccccca cctggagcaa tacctgaaat cccacagggg gaaccctcat ggggtgatat
     2221 tgccactgac ctgcttgaat cggcagcgga aaaagcagta ccactggcga aggcctgggg
     2281 gaacgctgtt atcaccctga cggaaagcaa tgccggtttt atggatcgcg tcagcgccgg
     2341 cgcatcgctt ctgtttcccg ccggtccggt attaatggag tttgccacca tggtgggcgg
     2401 gcgtagcgaa atcaaaaaag atgtggattt cccggaagcc ggtgaggaca cggcgctctg
     2461 cgacaaggag aacaaaccac cgaggatagc ccagggcagt agcaacgtct ttatcaacaa
     2521 tcagcctgcc gcgcgcaagg gcgacaaact ggagtgcagt gcggcgatcg tgggaggttc
     2581 gccggacgtc tttattggcg gtgaacaggt gacctatctg gatatccagc cggagttccc
     2641 gccgtggcag agaatgatcc tggggggaat aacgatagcc agctatcttc tgccgcctgc
     2701 gggactgctg ggaaaactga agaatctggc gagactcggc aaactgggaa acctgctggg
     2761 gaaaagcggg aagctgctgg gcgcaaagct cggcgcgttg ctggggaaaa caggtaagtc
     2821 gttaaaaagt attgccaata aagtcatcag atgggtaaca gatcctgtcg atccggtaac
     2881 cggcgcatac tgcgacgaac gtaccgactt caccctgggc cagaccctcc ccctctcctt
     2941 cacccgtttc cacagttctg tactgccgct gcatggcctg accggcgtgg gctggagcga
     3001 ctcctggagc gaatacgcct gggtgcgtga acagggaaat cgggtagata ttatcagcca
     3061 gggagccacg ctgaacttcg ccttcgacgg tgacagtgat acggcggtta acccgtatca
     3121 cgcccagtac attctgcgcc gccgcgacga ttacctggag ctgttcgaca gggatgcact
     3181 gagcagccgc ttcttttatg acgcctttcc gggaatgcgt ctgcgccacc cggtgactga
     3241 cgataccagc gatgaccgcc tggcacacag ccccgacgac cggatgtata tgctgggcgg
     3301 gatgagcgac accgccagca accgcatcac gtttgagcgc gacagccagt accggatcac
     3361 gggtgtcagt cacaccgacg ggatccggct taaactgacg taccacgcca gcggctacct
     3421 gaaagccatt caccgcacgg ataacggcat acagacgctg gcgacctacg aacaggatgc
     3481 gcgggggcgg ctgacagaag cggatgcgcg gctggactac cacctgtttt atgagtacga
     3541 cgctgcggac cggatcatcc gctggtccga taacgaccag acgtggagcc gtttcaccta
     3601 cgatgcacag ggccggtgcg tgaatgtcac cggggcggag ggctattaca acgccacgct
     3661 ggactatggt gacggctgca ccaccgtgac ggacggcaag ggcactcacc gttattacta
     3721 tgatcctgac ggcaatattc tgcgggaaga agcgccggac ggcagtacca ccacgtatga
     3781 atgggatgaa ttccatcacc tgctggcccg ccactcccct gccgggcggg tggagaagtt
     3841 tgaatacaac gccgcactcg gtcagttaag ccgttacacg gcggcagacg gcgcggagtg
     3901 gctgtaccgc tatgatgagc gcggcctgct cagcaacatc accgaccctg ccgggcagac
     3961 atggacgcag cagtgtgatg agcgcggcct gccggtaagc ctggtgtcgc cacagggcga
     4021 agagacccgg ctggcataca ccgctcaggg gctgctgtcg gggatattcc gccaggatga
     4081 acggcgtctg ggcatagagt acgaccacca caaccggccg gaaacactca ccgacgtgat
     4141 gggccgtgaa caccacaccg aatacagcgg tcacgacctg ccggtgaaga tgcgcggccc
     4201 cggcggtcag tcagtgcggt tacagtggca gcagcaccat aaactgagcg gccttgagcg
     4261 ggcagaaacc ggcgcagaag gattccgcta tgaccgccac ggcaacctgc tggcgtacac
     4321 ggacggtaac ggcgttgtct ggacaatgga atacggcccg ttcgatttgc cggtggcgcg
     4381 aacggacggt gaaggccacc gctggcagta ccgctacgat aaagacacgc tgcagctcac
     4441 agaagtcatt aacccgcagg gcgagtcata ccgttatatt ctggacaact gtggccgggt
     4501 gacggaagag cgtgactggg gcggcgtggt ctggcgttac cgctatgacg ctgatggcct
     4561 gtgtaccgcc agggtcaacg gcctggagga aaccatcctc tacagccggg acgccgcagg
     4621 ccgcctggca gaagtcatca cgccggaagg caaaacgcag tatgcgtatg acaaatccgg
     4681 caggctgacg ggtatcttca gcccggacgg cacatcacag cgcaccggct atgacgaacg
     4741 cgggcgggtg aatgtcacca ctcagggccg acgggccatt gaataccact accccgatga
     4801 acacaccgtt atccgctgta tcctgccacc ggaagatgaa cgcgacagac accccgatga
     4861 atccctgctg aaaaccacgt accgctacaa cgccgccgga gaactgacag aggtcattct
     4921 gccgggggat gagacgctga cgttcagccg tgatgaggcg ggacgtgaag tgctccggca
     4981 cagtaaccgg ggttttgcct gtgaacaggg ctggaatgca gccggccagc ctgtcagtca
     5041 gcgcgccgga ttttttccgg aggaaaccac atggggcggg ctgctcccct cactggtacg
     5101 ggagtaccgt tacgacagcg cgggcaatgt gtcggctgtc accagccggg aagattacgg
     5161 acgggaaacc cggcgggagt accggctgga ccggaacggc caggtcacgg cggtgacagc
     5221 ctcaggcacc gggctgggct atggcgaagg cgatgagtcc tatggctatg acagttgtgg
     5281 ctacctgaag gcgcagtctg cgggcaggca ccggataagt gaagagactg accagtatgc
     5341 cggaggccac cggctgaaac aggccggaaa cacgcagtat gactatgacg ccgcaggccg
     5401 gatggtcagc cgcaccagac accgtgacgg ctaccgcccg gaaacagagc ggttccggtg
     5461 ggacagccgg gaccagctga ccgggtattg cagcgcacag ggtgagcagt gggaataccg
     5521 ccacgacgcc agcggcagac gaacagagaa acgctgcgac cggaagaaaa tccgcttcac
     5581 gtacctgtgg gacggcgaca gtattgcgga aatccgggaa taccgcgatg ataaactgta
     5641 cagcgtacgg cacctggtgt ttaacggctt tgagctgata agccagcagt tcagccgggt
     5701 acggcaggcg catccgtcgg tggccccgca gtgggtgacg cggacgaatc atgcggtgag
     5761 cgacctgacg ggccgcccgc tgatgctctt taacagtgaa ggtaaaaccg tctggcggcc
     5821 gggacagacc agcctgtggg ggctggcact cagcctgccc gcagacaccg gctacccgga
     5881 cccgcgcggg gagtgggacc cggaagccga ccccggcctg ctgtatgcgg gacagtggca
     5941 ggatgcggaa tcggggctgt gctataaccg gttccggtac tacgagccgg aaaccggaat
     6001 gtacctggtg agtgatccgc tggggttgca ggggggtgag cagacttacc ggtatgtgcc
     6061 taatccactg gggtatattg atccgctggg gctggcaaaa acttctgttc ccgcagaaaa
     6121 aataagcctt tcggataaag caagagatct ttttagacaa ggaaaagtaa gagaagcatt
     6181 agatgttcat tatgaggatc ttgtacgaag aaagcttggt ggtatatctc aagaaattgc
     6241 cgggcgtgaa tatgatgttg ttactgacaa gatcatagcg caggtcaaga ggacttatag
     6301 ctctatagat aatccaaaaa acttcttaag taagtctact cggacacaaa ttaagaagac
     6361 aattgagctg gctgaagaac aggggaaaga ggctcagttt tggtttaaat atggagtaag
     6421 ccctaaagta agagaatata tagaaagcaa aggtgggaaa gttatattgg gtatggggaa
     6481 ttaacatgtc aagatatata cttattagag gttggtttga gtgcgacttt gaacaagtaa
     6541 aaaaaataaa agaagctaat gaagaatttt tcaaacattc tgaaaaatat ttattaccag
     6601 aaaacacgaa acgactttat ttaaatgggt ggtgttaccc agaatatcct atcaactggg
     6661 ttagcgttat ttccttaggt gttaatataa atcaaattgc atttgattat ataaaagaca
     6721 tgattgtaaa tgtttcaaaa actgaaagat tggatggtta ttttcgtgtg aatgatgatg
     6781 aagataattt attcttagaa tggattgtaa aagatggtta tttagaggaa ataattttgt
     6841 ctggttcata aattcatctt ctttgttgag aagattaact gcctaggtga aattatcctc
     6901 tatgaccggg acgctgcagg ccacctggca gaagtcatca cgcaggaaga caagtcgctg
     6961 tatccctttg aaaaatccgg caggctaatg ggcatcttca gcccaggcgg cacatcacag
     7021 cgaatttgaa ggcaaatgcg aaaattagtg atcttgaagt tcatacagta ttggtaaaaa
     7081 caggcgaagc ttttccaatg tgtaagaatt ttcaaatttc aatatttggc gctgttgtta
     7141 caagcgggtg attatatgtg tgaattaatt aatgaagcat caaaatatgg gaaagtcaat
     7201 gaaagaaaga tggacattga tttgtgctgt aaaaaaatag aggaagaagg atattctgtt
     7261 ttcgttg
//
LOCUS       DAZGOB010000188         7210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGOB010000188 DAZGOB010000000
VERSION     DAZGOB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.188"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..181
                     /locus_tag="ACHLIF_004648"
     CDS             <1..181
                     /locus_tag="ACHLIF_004648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608504.1"
                     /translation="RNISYALKLNYKGGQTPSAHQERPGCFPGVFRQPLTPIMGSIPT
                     FIPEMNTGFNLLNKK"
     gene            305..1432
                     /locus_tag="ACHLIF_004649"
     CDS             305..1432
                     /locus_tag="ACHLIF_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO9608505.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            1438..2229
                     /locus_tag="ACHLIF_004650"
     CDS             1438..2229
                     /locus_tag="ACHLIF_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO9608506.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(2265..3190)
                     /locus_tag="ACHLIF_004651"
                     /pseudo
     CDS             complement(2265..3190)
                     /locus_tag="ACHLIF_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(3335..4684)
                     /gene="lysC"
                     /locus_tag="ACHLIF_004652"
     CDS             complement(3335..4684)
                     /gene="lysC"
                     /locus_tag="ACHLIF_004652"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO9608507.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     regulatory      complement(4776..4972)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5209..6858
                     /gene="pgi"
                     /locus_tag="ACHLIF_004653"
     CDS             5209..6858
                     /gene="pgi"
                     /locus_tag="ACHLIF_004653"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO9608508.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALVGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1838 a         1710 c         1855 g         1807 t
ORIGIN      
        1 tcgcaatatc tcgtacgcgt tgaaactgaa ttacaaaggg ggtcaaaccc cgtcggcaca
       61 ccaggaacgg ccaggctgtt ttccgggcgt gttccgtcag cctttgacgc caattatggg
      121 ttcgattccc acattcattc ctgaaatgaa tactggtttt aacttattaa ataaaaagtg
      181 agggattgcg cagtcagcga tccccgataa cgtcgccgat aaagatgccg ggtaacaggc
      241 aaaagggaat tcccctgtcg cttcgtgtct gcttccgcga cggctgttaa gcaaggaata
      301 gataatgatt aaaaaaataa gcgtaagaaa agatcagctg gcattactct ccagaaacgg
      361 cgattactac aaagtgctac atgcaggcga gcatctttta ccctggttaa atacgccaga
      421 agtcttgctg attacgctcg atggcagcga agttcccgac gtgctggcgg attatttgcg
      481 ccgctttcag cctgattggg tggagaaata ttgcctggtg gcagacttgt cggagattga
      541 agccggggcg ttgtatatgg acggtatttt gcaggagatc ctgccgccat caacgcgccg
      601 tttgtactgg cgtgtagagg atgatttgac actggtgcgg atgaatacgc agcaagtcca
      661 ggtacaaacg gaggtgatga atgcggtatt gcaaccacgg cgcaaagggg cagttaaagg
      721 ccgcgatgca atactaacgg tgcaggttcc ggcctggcat gtaggcgtgc tgaaaatcga
      781 tggtgaaacg caggcgctgt taccgccggg gctgaccgca tactggaaaa ttaaccatct
      841 ggttgacgct gaagtggttg acacccgctt gcaggtactg gaagtcagcg ggcaagaaat
      901 cttaacgaaa gataaggtca atctgcggat taacctcgcc gctaactggc gctacagcga
      961 cgtgttattg gcatttagcc agttaacgaa gccgattgat catttgtatc gggaattaca
     1021 atttgccctg cgtgaagcgg taggtacgcg tacgttggat gaattgctgg aagataaaca
     1081 ggtcattgat gacgttgtca gcgaacaggt gaaatcgcgc atgttgccat ttggtatgga
     1141 gattgcctcg ttaggggtga aagatatcgt cctgcctggc gatatgaaaa atatccttgc
     1201 gcagctggtt gaggcggaga aatccgcgca ggccaatgtg attcgccgac gtgaagaaac
     1261 ggcagcgacg cgttcgttac tcaataccgc taaagtcatg gaaaacaacc cggtagcgtt
     1321 acgcttaaaa gaactggaaa cgctggagag agtggctgaa cggatcgata acatctctgt
     1381 atttggcggt ctggatcagg ttctgcatgg gttggttaat atcaaagggt gagtaacatg
     1441 ccattaaatg gagtcagtgc tgcgatgcga gaaagggtat cgcagcagtt aaaagaaatt
     1501 gaacggcggt atgatgtgaa agtactgtac gcctgtgaat cagggagccg cggctgggga
     1561 tttgcctcgc ccgacagcga ctacgatgta cggtttttgt atgttcaccc gctggaatgg
     1621 tatctgcgag tagagtcacc gcgcgatgtt attgagctgc ccatcgacga cgagttagat
     1681 gtttccggtt gggagtggcg taaggcgctg ggattattga aaggcgcgaa cccgacgctg
     1741 atagaatggc tggattctcc ggtggtttat caacaagatg aagaaacgat aacggcgttt
     1801 aaggcaatgg tcccaatgtg gttttctccg ctgcgtgcgc gctggcatta ctattcgatg
     1861 gcgcagaaaa acttccccgg atatttgcag ggcgacgaag ttcgactgaa aaaatacttt
     1921 tacgtgctgc gtccgctgct ggccgtgcgc tgggtcgaag cgggtaaagg cgtaccgcca
     1981 atgcgctttg ctgagttact ggcgggaagc gaacttgatg cgccgttacg tgcagaaata
     2041 gacgaattac tggaacgtaa acagcgcgca ggagaagcgg aatatggtcc gcgccgtcca
     2101 ttgttacatg cgtttattcg tgcggaactg gcgcgtgggg aaattccacc gctgttaccg
     2161 gatagcagag aaggggacgt taaggaactg gatagcttaa tgtaccagac ggtaatgcgc
     2221 cgggcataaa gcccggcgtt atttcacgcc atcagctctt attattattg tgtattcccg
     2281 ctgatttcat tctgtataag cgttgcaata tcgtcattcg ttgccgcttg tgaactaaat
     2341 atcatcttct cacgcgaata caccgcaaac ttatctgaca ggttcgaagc aaacaccttg
     2401 cgggcatttt ctgccagcaa accggtgacg atatcatggt ggtttttatc cacttttcgg
     2461 gcaatatttc ccgcctcttt gtccttacta taccgttcat tggtgctttc ccagcttaat
     2521 gccgctgagg ttgtttttat catgacttct gtttggtagc ctgcatcttt gagtttttta
     2581 atgtttgtaa cggcgtcgcg gcattacgaa atgttccttc gacaacaatg ttgagtttac
     2641 tgactatcgc tctctcaatt gcacgttcga ccgtttcgcc ggaaaatttc gctgtatggg
     2701 taacaaaatc atccccataa ttttggtaga tataattaaa atcagggtgt agaaatcgaa
     2761 aatcatcacc gttaataatc attatattat cttttgtctc tcttttggct aattcaatca
     2821 gagatgattt acctgctccg ggttgcccac ctaaaacaaa accaatggga tgctgttgcg
     2881 gtgtgacttc atgatttgtc agacgctgcc aaatagaatc aaaagatgct ttattttctg
     2941 aatgcgtagt attgttgaat tcatgctgat attgagttct tacctgctga aggagaatct
     3001 taccttgtgt aaaatcaaca agttcagcgg gggtagaata aagataatcg cgatagctga
     3061 ctaatcgctt gagagcatcg cctgaatcct gatgcgacga aactgagcaa ttatcaatga
     3121 tatttgataa aaacgcgatg cactgactgg ctccgtttaa aagtgcatct ttttccatta
     3181 aagtagccat taacactcct gttaaatgat atttttcggt gattataata ctgttctaca
     3241 ggagtgattt tgcttttcag atattattta atctatgttt tcagtgagga tggcgtataa
     3301 caatcggtga gcgttatacg ccagtaggga aatattactc aaacaaatta aaatgcagct
     3361 tctgcaccac ctgctcggca tcttcgccgg gcaccaggaa gcacaggtta tggctggatg
     3421 cgccgtaaca aatcatgcga atgttgaacg gttccagtac gccgaatacc tctttgccaa
     3481 cgccgcaggc ttttgacagg tcattgccaa tcaacgcgac cagcgccaga ccttcttcta
     3541 cttccacccg gcacagtgcg gaaagctcca tcagcagaga ttgcgtcagc aacgtatcgc
     3601 cagtggaggt tgaaccggtg gtatcaaggg ttaatgccac gctcacttct gacgtggtga
     3661 ttaagtctac cgaaatatta tgccgcgcga ggatgccgaa aacttccgcg aggaagccgc
     3721 gagaatgcag catattcagg ctgtgcaaag tgagcagagt ctgattgcga cgaagcgcca
     3781 gcgcgcggaa cagcggcgga ttttcagttt tattgcacac cagcgtacca cctgcgcgtg
     3841 ggtctttgct ggagccgaca aagaccggga tatcgctgcg tactgcgggt agcaacgttg
     3901 ccggatgcag tacttttgca ccaaaagttg ccatctctgc cgcttcggca aacgcgattt
     3961 catcaatgcg ttttgctgcg gaaactacgc gtggatcggt ggtgtagatg cccgggacgt
     4021 cggtccagat atcaacacga gatgcgtgta aagcctccgc cagcaaggct gccgtataat
     4081 cgctgcctcc acggccaagc gtcgttgtac gacctttatt ttcgctaccg ataaatccct
     4141 gggtgatcac taagccttca ttgagacgtg ggagcagctg cagcgcggcc agttccgcca
     4201 gcgcggctat atctggctct gcacgaccaa atcggtcgtt ggtacgcatc actttacgta
     4261 catcaaacca ctgtgcctga acatcgcgtt cgcgcaggat ctcaacaaac agcagggtcg
     4321 acatcagctc gccgtggctg accagctcat ctgtcagcgc cggagacgtt gccagcgccg
     4381 ccgcttctgc cagaacagta atgttctcca gcagacgttc aatctcttca cggataacgt
     4441 tcgggtaacg cagacgttcc agaatggcaa actggatgtt gcggatagcg tcgagttttt
     4501 cgaatcgctc gccaggttcc agtccttcag ctaaagcgac cagcagatta gtgataccag
     4561 cagaagccga gaggacaact aaacgcacgt tggcatcaga aagcacaata tcagcgctgc
     4621 ggttcatggc gtcaaaatca gctacgctgg taccgccaaa tttggagaca acaatttcag
     4681 acataactac ctcgtgtcag gggatccatt ttcagccttg gcacaaggga agagcggaag
     4741 acgggtgggc gcagagcgat acttcgctac tattttcacc cagaagtgct ccaccacttg
     4801 cgaaacgccc gactgcgaac gcttctggtg acaacccagg ggattcagcc cctgtagccg
     4861 atgatgaacg tggccagccg ttcaatcacc tcggcgatgc accccctcag gtgttatcac
     4921 aggactggct cctccaacac cgttacttgg gcaacgcgcc tcttctggcc tgcgctagcg
     4981 caggtagtac atttataaat aaagggtgag cggggcggtt gtcaacgacg gggtcatgcg
     5041 gatttttcat ccactcctgg cggtcagtag ttcagctaat aaatgcttca ctgcgctaag
     5101 ggtttacact caacattacg ctaacggcac taaaaccatc acatttttct gtgactggcg
     5161 ctacaatctt ccaaagtcac aattctcaaa atcagaagag tattgctaat gaaaaacatc
     5221 aatccaacgc agaccgctgc ctggcaggca ctacagaaac acttcgatga aatgaaagac
     5281 gttacgatcg ccgatctttt tgctaaagac ggcgatcgtt tttctaagtt ctccgcaacc
     5341 ttcggcgatc agatgctggt ggattactcc aaaaaccgca tcactgaaga gacgctggcg
     5401 aaattacaag atctggcgaa agagtgcgat ctggcgggcg cgattaagtc gatgttctct
     5461 ggcgagaaga tcaaccgcac tgaaaaccgc gctgtgctgc acgtagcgct gcgtaaccgt
     5521 agcaataccc cgattttggt tgatggcaaa gacgtaatgc cggaagtcaa cgcggtgctg
     5581 gagaagatga aaaccttctc ggaagcgatt atctccggtg agtggaaggg ttataccggc
     5641 aaagcaatca ctgacgtagt gaacatcggg atcggcggtt ctgacctcgg cccgtacatg
     5701 gtgaccgaag cactgcgtcc gtacaaaaat cacctgaaca tgcactttgt ttctaacgtc
     5761 gatgggactc acatcgctga agtgctgaaa aaagtaaacc cggaaaccac gctgttcctg
     5821 gtagcatcta aaaccttcac cactcaggaa accatgacca acgcccatag cgcgcgtgac
     5881 tggttcctga aagcggcagg tgatgaaaaa cacgttgcga aacactttgc ggcgctctca
     5941 accaatgcca aagccgttgg cgagtttggt attgatactg ccaacatgtt cgagttctgg
     6001 gactgggtcg gcggccgtta ctctttgtgg tcagcgattg gcctgtcgat tgttctctcc
     6061 atcggctttg ataacttcgt tgaactgctt tctggcgcac acgcgatgga caagcatttc
     6121 tccaccacgc ctgccgagaa aaacctgcct gtactgctgg cgctggttgg catctggtac
     6181 aacaatttct ttggtgcgga aactgaggcg attctgccgt atgaccagta tatgcaccgt
     6241 ttcgcggcgt acttccagca gggcaatatg gagtccaacg gtaagtatgt tgaccgtaac
     6301 ggtaacgttg tggattacca gactggcccg attatctggg gtgaaccagg cactaacggt
     6361 cagcacgcgt tctaccagct gatccaccag ggaaccaaaa tggtaccgtg cgatttcatc
     6421 gctccggcta tcacccataa cccgctctct gatcatcacc agaaactgct gtctaacttc
     6481 ttcgcccaga ccgaagcgct ggcgtttggt aaatcccgcg aagtggttga gcaggaatat
     6541 cgcgatcagg gtaaagatcc ggcaacgctt gactacgtgg tgccgttcaa agtgttcgaa
     6601 ggtaaccgcc cgaccaactc catcctgctg cgtgaaatca ctccgttcag cctgggtgcg
     6661 ttgattgcgc tgtatgagca caaaatcttt actcagggcg tgattctgaa catcttcacc
     6721 ttcgaccagt ggggcgtgga gctgggtaaa cagctggcga accgtattct gccagagctg
     6781 aaagatggca aagaaatcag cagccacgat agttcgacca atggtctgat taaccgctat
     6841 aaagcgtggc gcggttaatc atcgtcgata tgtaggccgg ataaggcgtt cacgccgcat
     6901 ccggcaaccg atgcctgatg cgacgcggtc gcgtcttatc aggcctacag gtcgatgccg
     6961 atatgtacat cgtattcggc aattaataca tagcacgatt gattaaataa ccttaataac
     7021 aatgccgacg ttatgtcggc atttttttat cagataaatc cccttgtctg tgatttaacg
     7081 gaaatcatac catgaggtta atcctaaaat agatttttaa tcgttgttta tttcggaaaa
     7141 tacgcagatt aattgctttt gttttttatt ttaagtttat gatttttatt gttatttaaa
     7201 tataagttga
//
LOCUS       DAZGOB010000189         7181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGOB010000189 DAZGOB010000000
VERSION     DAZGOB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.189"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(306..1372)
                     /locus_tag="ACHLIF_004654"
                     /pseudo
     CDS             complement(306..1372)
                     /locus_tag="ACHLIF_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(1375..1737)
                     /locus_tag="ACHLIF_004655"
     CDS             complement(1375..1737)
                     /locus_tag="ACHLIF_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO9608509.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2274..4187
                     /gene="mtlA"
                     /locus_tag="ACHLIF_004656"
     CDS             2274..4187
                     /gene="mtlA"
                     /locus_tag="ACHLIF_004656"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO9608510.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSTILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGEYIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4417..5565
                     /gene="mtlD"
                     /locus_tag="ACHLIF_004657"
     CDS             4417..5565
                     /gene="mtlD"
                     /locus_tag="ACHLIF_004657"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO9608511.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5565..6152
                     /gene="mtlR"
                     /locus_tag="ACHLIF_004658"
     CDS             5565..6152
                     /gene="mtlR"
                     /locus_tag="ACHLIF_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO9608512.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6164..6373)
                     /gene="yibT"
                     /locus_tag="ACHLIF_004659"
     CDS             complement(6164..6373)
                     /gene="yibT"
                     /locus_tag="ACHLIF_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO9608513.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6658..7020
                     /locus_tag="ACHLIF_004660"
     CDS             6658..7020
                     /locus_tag="ACHLIF_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO9608514.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1823 a         1801 c         1824 g         1733 t
ORIGIN      
        1 ttttaaatgt gactgtcatc cttcagataa taaagccatc atcgcaatag atggaaaagc
       61 actcctgctc ttttttgatt agagccgtcg caggaggaac aactcatatc atcattgcga
      121 tctcgacgat gtatagtctc atcctgcggc agaacaagac ggataaaaaa tccaatgaga
      181 gcacactcat ccataactta ttaacataat gaatattaaa gaaaaatcat cgcagctaat
      241 gaagtaatat gcaaaaataa ttagtcctta caaggagcaa agttccataa tctcccccct
      301 ccccctcaat gatccaaata aagataatgc atccagctgg tcattcttag cagcactttc
      361 cgcatcaccg aaacatggct gaaatggtcg gagtaaaccg ccacctgggc gtagatgccg
      421 tcgggtaagg catcgatatc atcgttaggg tccagttcaa tggttcccag cacaccgtcc
      481 gtgccgggca cgaccgttaa tgattgcaat accccctgcg cctgataaga accgcctggc
      541 acgacaggta aaatactggt cagtttgccg tggaacacct gcccaggtag cgcgttaaac
      601 accacttctg catcatcacc aggtttcaga cgtaacagcg agttttgccg aaattgggcg
      661 acaatttgcc gtttttgctc ggggatgaaa accatcaccg gacgcaacgg caaggcagct
      721 gcgtatgtac ctgggcggat cagtacctga gtaatgtagc cattgctcgg cgcgcgaatg
      781 acagtctgct caagattata ttttgcttca gtaagttgcg ctcttaagct cacaatctgc
      841 gattgctcgc cgttaaccat actgtcgagc tggctctgga tctgcgcctg ctccgccacc
      901 gagcctttca ccagcgcatc ctgcgcgagg aaattttgcc gcgcatcgtc gatgtcacgt
      961 tccgagaacg gattcaccgc cgcctggctg cctttcagat aacgttgata atttttaaac
     1021 agacggtcgc gctccgctga aacctgggtg gtgttggcct gcgcttctgt gagctgcgcg
     1081 cgcagagtct ttatattatg cgtcgccgtc atcaggtcag cctgaagcct gtcaactcgc
     1141 gcctggtaac gaaccgggtc gagcttaaaa agcacctcgc ccttttgaat aagctgatta
     1201 ttcttgtcag tgacttcagt aacaattccc gtcacctgtg gcgtgatggg gatcgctatc
     1261 actgcctttt gcgcggtaaa agtgtaaggg tggttgtagt tcatcaacaa aatcaaacca
     1321 ctcaccagaa acacgccagc gccacgtaag ttaaaacaat caatagatcc ataattagat
     1381 ctccgggaac gtggaatagt cagtcttttt ctcggcggcg agttgatgct ccagccggga
     1441 aattcggtca gaaagtgcgg cgatttccgg gtcagtcgct ttctcctgcg acgcaacatg
     1501 tgactgcata ccccagccac gctccggttg atagagcgtc gcccagatcc acagaaacgg
     1561 ccagataaca tgcagagtaa acaggctcac ccagcccgcc gtatgaatag cgtcggcatg
     1621 gggatggttg cgctttttgg caatcagata agggatgtca tgtatcgcga tgattccata
     1681 aaaaatcacc agaaagacga agatcagcac tcccaacgcg aaatagttta gaaacatatc
     1741 tgcctcggat tcacgtttat cagtgttgtt tttgggctgg cagccagaag ggagtcaggc
     1801 tgatattttg acaataatcc gggttcgcga ttctcgccat aacaccaaag aataattttt
     1861 agaggtgatg agttgcttag ttacataacg attgtatgac gaaggcataa catgctgtag
     1921 atcacatcag gtgaacgccg taagaaaata tcttgtgatt cagatcacaa agattcaaca
     1981 aaccatcaaa acaaaaatgt gacactactc acatttaaat gccattttta gcgaaaatcg
     2041 ccgccttgtt gcttttttac acaagcgttt tgtgatgaac gtcacgtcaa ttacctctct
     2101 accccctata tttatgtgat tgatatcaca caaaaggccg tcgactggac agttaaccga
     2161 ttcagtgcca gatttcgcag tatctacaag gtccggctac ctctgccgcc acattaacaa
     2221 aaaacctcgg gcttccagcc tgcgcgacag caaacataag aaggggtgtt tttatgtcat
     2281 ccgatattaa gatcaaagtg caaagctttg gtcgtttcct cagcaacatg gtgatgccaa
     2341 atatcggcgc gtttatcgcg tggggtatca tcaccgcgtt atttattcca acagggtggt
     2401 taccgaacga gacgctggcg aagctggtcg ggccgatgat cacttatctc ctgccgctgc
     2461 tgatcggtta taccggtggt aagctggtag gcggcgaacg tggcggcgta gtcggtgcca
     2521 tcaccaccat gggcgttatc gtcggcgcag acatgccgat gttcctcggt tctatgattg
     2581 caggtccgct gggcggctgg tgcattaagc acttcgaccg ctgggtagac ggtaagatca
     2641 aatccggttt tgagatgctg gtgaataact tctccgcagg catcatcggg atgatcctcg
     2701 ctattctggc attcctcggc attggcccga ttgttgaagc cctgtccaaa atgctggctg
     2761 cgggcgttaa cttcatggtt gtccatgaca tgctgccgct ggcgtctatc tttgttgaac
     2821 cggcgaaaat cctgttcctc aacaatgcca ttaaccacgg tatcttctcg ccgctgggta
     2881 ttcagcagtc ccatgaactg ggtaaatcaa tcttcttcct gattgaagct aacccaggtc
     2941 caggtatggg cgtgctgctg gcgtacatgt tctttggtcg tggtagcgct aaacagtctg
     3001 cgggcggtgc ggcaatcatc cacttcctgg gtggtatcca cgaaatctac ttcccgtatg
     3061 tgctgatgaa tccgcgtctg atcctcgcag tcatcctcgg cggtatgact ggcgtgttca
     3121 cgctgactat cctgggcggt ggtctggttt ctccggcatc tccgggttct atccttgctg
     3181 tactggcgat gacaccaaaa ggtgcttatt tcgctaacat cgcgggtgtg tgtgcggcga
     3241 tggctgtctc cttcgttgtc tctactattt tgctgaaaac cagcaaagtg aaagaagaag
     3301 atgatattga agcagcaact cgtcgtatgc aggacatgaa agctgagtct aaaggcacat
     3361 ctccgctgtc tgcgggcgat gtgactaacg acctgagcca cgtacgtaaa atcatcgttg
     3421 cctgtgacgc cggtatgggt tcaagtgcga tgggcgcagg cgtgctgcgt aagaaaattc
     3481 aggatgcagg tctgtcacag atttcggtca ctaacagcgc gatcaacaac ctgccgccag
     3541 atgtggacct cgtcatcact caccgtgacc tgaccgaacg cgctatgcgc caggttccgc
     3601 aggcgcagca tatttcgctg accaacttcc tcgacagcgg cctgtacacc agcctaaccg
     3661 aacgtctcgt tgctgcccag cgccatactg aaaacgaagt gaaagtgaaa gacagtctga
     3721 aagacagctt tgacgattcc agtgctaacc tgttcaaact gggcgcggag aacatcttcc
     3781 tcggtcgcaa agcagcaacc aaagaagaag cgattcgttt tgctggcgag cagctggtga
     3841 aaggcggtta cgttgagccg gaatacgttc aggcgatgct ggatcgtgaa aaactgaccc
     3901 cgacttatct gggtgagtat atcgcggtgc cacacggtac ggttgaagcg aaagatcgcg
     3961 tactgaaaac gggcgtggtg ttctgccagt acccggaagg cgtgcgcttc ggtgaagaag
     4021 aagatgacat tgcccgtctg gtgattggta ttgcagcccg taacaacgag cacattcagg
     4081 ttatcaccag cctgaccaat gcactggatg atgagtccgt catcgagcgt ctggcacaca
     4141 ccaccagcgt ggatgaagtg ctggaactgc tggcaggtcg taagtaatcc aatcccaccc
     4201 tctccacatg gagaaggtgg ggttaattgc ctgatgcgct acgcttatca ggcctacagg
     4261 atgcatcaca atttgttgaa tttgcacgtt cttgtaggcc ggataaggcg cttacgccgc
     4321 atccggcgct gcccctctcc tcacggagag ggtttgggtg agggaaaagc ctcaccccag
     4381 ccctctcggg taaaaacatt gatgaaggtt aatactatga aagcattaca ttttggcgca
     4441 ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag acgcgggtat ccaactgacg
     4501 tttgccgatg tcaatcaggt ggtacttgat gccctgaatg cccgtcatag ctatcaggtt
     4561 catgtggttg gtgaaaccga gcaggtagat accgtttccg gcgtcaatgc tgtcagcagc
     4621 attggtgatg atgtcgttga tctgattgct caggtcgatt tagtcactac cgccgttggc
     4681 ccggttgtgc tggaacgtat tgctccggct atcgccaaag ggctggtgaa acgcaaagaa
     4741 caaggtaatg aatccccgct gaacatcatc gcctgtgaaa acatggtacg cggtaccacg
     4801 cagctgaaag gccatgtgat gaacgccctg ccggaagacg ccaaagcgtg ggtagaagaa
     4861 cacgttggct ttgtcgattc cgccgttgac cgcatcgtac cgccttcggc ttcggcaact
     4921 aacgatccgc tggaagtgac ggtagaaact ttcagcgaat ggattgtcga taaaacgcag
     4981 ttcaaaggcg cactgccgaa catcccaggc atggagttaa ccgacaacct gatggcattt
     5041 gtcgaacgta aactcttcac cctgaacacg ggtcatgcta taaccgcgta cctcggaaaa
     5101 ctggccggtc atcagaccat tcgtgacgct attctcgacg agaaaatccg cgcggtggta
     5161 aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc gctacggctt tgacgcagac
     5221 aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg agaacccgta tctgaaagat
     5281 gatgtagagc gcgtaggccg tcagccgctg cgtaaactga gtgctggcga ccgtctgatc
     5341 aagccactgc tcggtacgct ggaatacagc cttccgcaca agaatctgat tcaggggatt
     5401 gctggtgcaa tgcacttccg cagtgaagat gatccgcagg ctcaggaact ggcagcactg
     5461 atcgctgaca aaggtccgca ggcggcgctg gcacagattt ccggtcttga tgccaacagc
     5521 gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc aataatggtg gaccaggcgc
     5581 aggacaccct gcgcccgaat aacagattgt cagatatgca ggcaacaatg gaacaaaccc
     5641 aggcctttga aaaccgtgtg cttgagcgtc tgaatgctgg caaaaccgtg cgaagctttc
     5701 tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct tctggtgctt caggtattcc
     5761 gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt actcgacggc gacggtccgc
     5821 tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt gggcgtcatt aaccgccagg
     5881 aatacgaaga tgcggagctg ctgatggcat tgcgtgaaga actaaatcac gacggcaacg
     5941 agtacgcctt taccgacgac gagatccttg gaccctttgg tgaactgcac tgcgtggcgg
     6001 cgttaccacc gccgccacag tttgaaccag cagactccag tttgtatgca atgcaaattc
     6061 agcgctatca acaggctgtg cgatcaacaa tggtcctttc actgactgag ctgatttcca
     6121 gaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc tgcttactgc ccctctacct
     6181 gcttcggccg ataaagccga cgataatact ccagacgttg tagatataac ggcacgcttt
     6241 catcgggtat tccggacgga atcgcgttac ggggaggaag ttttttcaga tactcccgga
     6301 acgcctggct tgatgccatg aaatctacgg ctttatcgat aagaagcgga acgttttcac
     6361 ctaatttgcc catgatgata tctccgtatt accccgcgcc gggaatgcgc ggccgccaat
     6421 tttagtttag gttcccggta aagatgacat taaggaaaac gtgctgaatc ctcaataatc
     6481 atccgctgct gtttattatc ttttttctat agatttctta attaatcaac gaattgcgta
     6541 gtttttgagc atgttaatga ttacgttgca gaactattac aatgtcatca gttgtgaaca
     6601 aagcacctgg tcgcgcatac taggggctat aaatttatct ttatcagaag ccatcacatg
     6661 aaagaagtcg aaaaaaacga aatcaaacgt ctcagcgatc gcctggacgc catccgccac
     6721 cagcaggccg atctgtcgct ggttgaagcc gcagacaaat atgccgagct ggaaaaagag
     6781 aaagccacgc tggaagcaga aattgctcgc ctgcgtgagg ttcatagcca aaaactgagt
     6841 aaagaagcac agaagctgat gaagatgccg tttcagcgcg caattaccaa aaaagagcag
     6901 gctgatatgg gcaagctgaa gaaaagtgtt cgcggactgg tggttgtgca cccaatgacc
     6961 gcgctgggcc gtgaaatggg cctgcaggag atgactggat tctctaagac tgcgttttaa
     7021 gcgcctcaga cgactggttc gatcatatca acccgcatct ggcttgcctt agctggtcaa
     7081 tgcggggact ctcctgtcta ctcgcctaat taccaaacaa ttcactccct ttacgtcgat
     7141 ttaccgctgg ttgatattct gaaaatgcat ttttaccgaa a
//
LOCUS       DAZGOB010000190         7092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGOB010000190 DAZGOB010000000
VERSION     DAZGOB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.190"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            572..1561
                     /gene="araF"
                     /locus_tag="ACHLIF_004661"
     CDS             572..1561
                     /gene="araF"
                     /locus_tag="ACHLIF_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO9608515.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1631..3145
                     /gene="araG"
                     /locus_tag="ACHLIF_004662"
     CDS             1631..3145
                     /gene="araG"
                     /locus_tag="ACHLIF_004662"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO9608516.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3160..4146
                     /gene="araH"
                     /locus_tag="ACHLIF_004663"
     CDS             3160..4146
                     /gene="araH"
                     /locus_tag="ACHLIF_004663"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO9608517.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4313..5113
                     /gene="otsB"
                     /locus_tag="ACHLIF_004664"
     CDS             4313..5113
                     /gene="otsB"
                     /locus_tag="ACHLIF_004664"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO9608518.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5088..6512
                     /gene="otsA"
                     /locus_tag="ACHLIF_004665"
     CDS             5088..6512
                     /gene="otsA"
                     /locus_tag="ACHLIF_004665"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO9608519.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6519..6947)
                     /gene="uspC"
                     /locus_tag="ACHLIF_004666"
     CDS             complement(6519..6947)
                     /gene="uspC"
                     /locus_tag="ACHLIF_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO9608520.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1797 a         1642 c         1833 g         1820 t
ORIGIN      
        1 gaattttaat gatagcgctt tttgtatctt gttgatatta atcaatgaat ttgaattcat
       61 gatgaagtga tgattttata aaacgttttt tcatttttgc gagatggctc tcattatacg
      121 tgttctgtta attaatgagt acagtgataa ttaatggcga aatgaatgac gtgcattttc
      181 cacatctttg agttgcggtt attacaccat ttcaaaaact caacgccagg taatgcggcc
      241 tattgactgg ttaaaaagaa gacatcccgc atgggtacca aagacaacaa ggatttccag
      301 gctaatctta tggattaatc tgctgtgcat tcgacaattt gtctgacaaa ttggctttcc
      361 cttatgtctt ttcccgctaa atttatgcac gttctcactg taattctgcg atgtgatatt
      421 gctctcctat ggagaattaa tttctcgcta aaactatgtc aacacagtca cttatctttt
      481 agttaaaagg taatgctttg ttttccgatt aatttaacga atgtcattcg tttttgccct
      541 acacaaaacg acactaaagc tggagagaac catgcacaaa tttactaaag ccctggcagc
      601 cattggtctg gcagccgtta tgtcacaatc cgctatggcg gagaacctga agctcggttt
      661 tctggtgaag caaccggaag agccgtggtt ccagaccgaa tggaagtttg ccgataaagc
      721 cgggaaggat ttagggtttg aggttattaa gattgccgtg ccggatggcg aaaaaacatt
      781 gaacgcgatc gacagcctgg ctgccagtgg cgcaaaaggt ttcgttattt gtactccgga
      841 ccccaaactc ggctctgcca tcgtcgcgaa agcgcgtggc tacgatatga aagtcattgc
      901 cgtggatgac cagtttgtta acgccaaagg taagccaatg gataccgttc cgctggtgat
      961 gatggcggcg actaaaattg gcgaacgtca gggccaggaa ctgtataaag agatgcagaa
     1021 acgtggctgg gatgtcaaag aaagcgcggt gatggcgatt accgccaacg aactggatac
     1081 cgcccgccgc cgtactacgg gatctatgga tgcgctgaaa gcggccggat tcccggaaaa
     1141 acaaatttat caggtaccta ccaaatctaa cgacatcccg ggggcatttg acgctgccaa
     1201 ctcaatgctg gttcaacatc cggaagttaa acattggctg atcgtcggta tgaacgacag
     1261 caccgtgctg ggcggcgtac gcgcgacgga aggtcagggc tttaaagcgg ccgatatcat
     1321 cggcattggc attaacggtg tggatgcggt gagcgaactg tctaaagcac aggcaaccgg
     1381 cttctacggt tccctgctgc caagcccgga cgtacatggc tataaatcca gcgaaatgct
     1441 ttacaactgg gtagcaaaag acgttgaacc gccaaaattt accgaagtta ccgacgtggt
     1501 actgatcacg cgtgacaact ttaaagaaga actggagaaa aaaggtttag gcggtaagta
     1561 atttgccgga aaaattcccc tctgcatgat gcagaggggg tgtgaacgac cagtgattca
     1621 cggagacgtt atgcaacagt ctaccccgta tctctcattt cgcggcatcg gtaaaacgtt
     1681 tcccggcgtt aaggcgctga cggatattag ttttgactgc tatgccggtc aggttcatgc
     1741 gttgatgggt gaaaatggcg caggaaaatc aactctctta aaaatcctca gcggcaacta
     1801 tgcgccaacc acgggttctg tagtgattaa tgggcaggaa atgtcctttt ccgacacgac
     1861 cgcagcactt aacgcgggtg tggcgattat ttaccaggaa ctacatctcg tgccggaaat
     1921 gaccgtcgcg gaaaacatct atctcggcca gctgccgcat aaaggcggca ttgtgaatcg
     1981 ctcattgctg aattatgagg cgggtttaca acttaaacat cttggtatgg atattgaccc
     2041 ggacacgccg ctgaaatatc tctccattgg tcagtggcag atggttgaaa tcgccaaggc
     2101 gctggcgcgt aacgctaaaa ttatcgcctt tgatgagcca accagctccc tttctgcccg
     2161 cgaaatcgac aatcttttcc gcgttattcg tgaactgcga aaagaggggc gggtgatctt
     2221 atacgtttct caccgtatgg aagaaatatt tgccctcagc gatgccatca ctgtctttaa
     2281 agatggacgt tatgtcaaaa cctttaccga tatgcagcag gttgaccacg acgcgctggt
     2341 gcaggcgatg gtcgggcgcg acattggcga tatctacggc tggcaacctc gtagttatgg
     2401 cgaggagcgc ctgcgtcttg atgctgtgaa agcaccaggc gtgcgtacgc caataagtct
     2461 ggcggttcgc agtggtgaaa ttgttgggct gtttggtctg gtaggggcgg ggcgtagcga
     2521 attaatgaaa ggcttgtttg gcgggacgca aatcaccgcc ggtcaggttt atatcgacca
     2581 acagccgatc gatattcgta aaccgagcca cgccattgcc gcaggcatga tgctctgccc
     2641 ggaagatcgc aaagcggaag gcattattcc cgtgcactcc gttcgcgaca atatcaacat
     2701 cagtgccaga cgtaaacatg tgctcggcgg ttgtgtaatc aacaacggtt gggaagaaaa
     2761 caatgccgat caccacattc gttcgctcaa catcaaaacg ccgggcgctg agcaactgat
     2821 catgaatctc tcaggcggaa atcagcaaaa agccattctg ggccgctggt tatcggaaga
     2881 gatgaaggtc attttgctgg atgaacctac gcgcggcatt gatgttggtg ctaagcatga
     2941 aatttacaac gtgatttatg cgctggcggc gcagggtgtg gcggtgctgt ttgcctccag
     3001 cgacttacct gaagtcctcg gcgttgccga ccggattgtg gtgatgcggg aaggtgaaat
     3061 cgccggtgaa ttgttacacg agcaggcaga tgagcgtcag gcactgagcc ttgcgatgcc
     3121 taaagtcagc caggcagttg cctgagtaag gagagtatga tgtcttctgt ttctacatcg
     3181 gggtctggcg cacctaagtc gtcattcagc ttcgggcgta tctgggatca gtacggcatg
     3241 ctggtggtgt ttgcggtgct ctttatcgcc tgtgccattt ttgtcccaaa ttttgccacc
     3301 ttcattaata tgaaagggtt gggcctggca atttccatgt cggggatggt ggcgtgtggc
     3361 atgttgttct gccttgcttc cggtgacttt gacctttctg tcgcctccgt aattgcctgt
     3421 gcgggtgtca ccacggcggt ggttatcaac ctgactgaaa gcctgtggat tggcgtggca
     3481 gcggggttgc tgcttggcat tctctgtggc ctggtcaatg gctttgttat cgccaaactg
     3541 aaaataaatg ctctgatcac aacactggca acgatgcaga ttgttcgagg tctggcgtac
     3601 atcatttcag acggtaaagc ggtcggtatc gaagatgaaa gcttctttgc ccttggttac
     3661 gctaactggt tcggtctgcc tgcgccaatc tggctcaccg tcgcgtgtct gattatcttt
     3721 ggtttgttgc tgaataaaac cacctttggt cgtaacaccc tggcgattgg cgggaacgaa
     3781 gaggctgcgc gtctggcggg tgtaccggtt gttcgcacca aaattattat ctttgttctc
     3841 tctggcctgg tatctgcgat agccggaatt attctggctt cacgtatgac tagtgggcag
     3901 ccaatgacgt cgattggtta tgagcttatt gttatctccg cctgcgtttt aggtggcgtt
     3961 tctctgaaag gtggcatcgg aaaaatctca tatgtggtgg cgggtatctt aattttaggc
     4021 accgtggaaa acgccatgaa cctgcttaat atttctcctt tcgcgcagta cgtggttcgc
     4081 ggcttaatcc tgctggcagc ggtgatcttc gaccgttaca agcaaaaagc gaaacgcact
     4141 gtctgatgct tttttccaca acaatttaac gttttttccc accacagcca gccgccacaa
     4201 cggttggctg ttcttcattg caaatggcga cccccgtcac actgtctata cttacatgtc
     4261 tgtaaagcgc gttctgcgca aaacaataag aaaagagaag gaggagagcc gggtgacaga
     4321 accgttaacc gaaacccctg aactatccgc gaaatatgcc tggttttttg atcttgatgg
     4381 aacgctggcg gaaatcaaac cgcatcccga tcaggtcgtc gtgcctgaca atattctgca
     4441 aggactacag ctactggcaa ccgcaagtga tggtgcattg gcattgatat cagggcgctc
     4501 aatggtggag cttgacgcac tggcaaaacc ttatcgcttc ccgttagcgg gcgtgcatgg
     4561 ggcggagcgc cgtgacatca atggtaaaac acatatcgtt catctgccgg atgcgattgc
     4621 gcgtgatatt agcgtgcaac tgcatacagt catcgctcag tatcccggcg cggagctgga
     4681 ggcgaaaggg atggcttttg cgctgcatta tcgtcaggct ccgcagcatg aagacgcatt
     4741 aatgacatta gcgcaacgta ttactcagat ctggccacaa atggcgttac agcagggaaa
     4801 gtgtgttgtc gagatcaaac cgagaggtac cagtaaaggt gaggcaattg cagcttttat
     4861 gcaggaagct ccctttatcg ggcgaacgcc cgtatttctg ggcgatgatt taaccgatga
     4921 atctggcttc gcagtcgtta accgactggg cggaatgtca gtaaaaattg gcacaggtgc
     4981 aactcaggca tcatggcgac tggcgggtgt gccggatgtc tggagctggc ttgaaatgat
     5041 aaccaccgca ttacaacaaa aaagagaaaa taacaggagt gatgactatg agtcgtttag
     5101 tcgtagtatc taaccggatt gcaccaccag acgagcacgc cgccagtgcc ggtggccttg
     5161 ccgttggcat actgggggca ctgaaagccg caggcggact gtggtttggc tggagtggtg
     5221 aaacagggaa tgaggatcag ccgctaaaaa aggtgaaaaa aggtaacatt acgtgggcct
     5281 cttttaacct cagcgaacag gaccttgacg aatactacaa ccaattctcc aatgccgttc
     5341 tctggcccgc ttttcattat cggctcgatc tggtgcaatt tcagcgtcct gcctgggacg
     5401 gctatctacg cgtaaatgcg ttgctggcag ataaattact gccgctgttg caagacgatg
     5461 acattatctg gatccacgat tatcacctgt tgccatttgc gcatgaatta cgcaaacggg
     5521 gagtgaataa tcgcattggt ttctttctgc atattccttt cccgacaccg gaaatcttca
     5581 acgcgctgcc gacatatgac accttgcttg aacagctttg tgattatgat ttgctgggtt
     5641 tccagacaga aaacgatcgt ctggcgttcc tggattgtct ttctaacctg acccgcgtca
     5701 cgacacgtag cgcaaaaagc catacagcct ggggcaaagc atttcgaaca gaagtctacc
     5761 cgatcggcat tgaaccgaaa gaaatagcca aacaggctgc cgggccactg ccgccaaaac
     5821 tggcgcaact taaagcggaa ctgaaaaacg tacaaaatat cttttctgtc gaacggctgg
     5881 attattccaa aggtttgcca gagcgttttc tcgcctatga agcgttgctg gaaaaatatc
     5941 cgcagcatca tggtaaaatt cgttataccc agattgcacc aacgtcgcgt ggtgatgtgc
     6001 aagcctatca ggatattcgt catcagctcg aaaatgaagc tggacgaatt aatggtaaat
     6061 acgggcaatt aggctggacg ccgctttatt atttgaatca gcattttgac cgtaaattac
     6121 tgatgaaaat attccgctac tctgacgtgg gcttagtgac gccactgcgt gacgggatga
     6181 acctggtagc aaaagagtat gttgctgctc aggacccagc caacccgggc gttcttgttc
     6241 tttcgcaatt tgcgggagcg gcaaacgagt taacgtcggc gttaattgtt aacccctacg
     6301 atcgtgacga agttgcagct gcgctggatc gtgcattgac tatgtcgctg gcggaacgta
     6361 tttcccgtca tgcagaaatg ctggacgtta tcgtgaaaaa cgatattaac cactggcagg
     6421 agtgcttcat tagcgaccta aagcagatag ttccgcgaag cgcggaaagc cagcagcgcg
     6481 ataaagttgc tacctttcca aagcttgcgt aggagcggtt aatctcccgt aagtggaact
     6541 aacagcacat cgacctcact tgaggcaata actcttttcg ccgagcagga tgctcgcgaa
     6601 aagaaactgt gattgtgatt accgcaaatc accaaatcga aatgatgttt gtgacatact
     6661 tccagaatat gttcgcttaa ctcaccgtag gcaataaacg tcttgtcaac gggatacccc
     6721 gcatcctgaa ttaacttatc aagaaagctt tgcgtttctt cttgcatcac gctgcgtaaa
     6781 tcttcaagca tcggcgcggc taattgattg tacatttccg ggtcggaagc gagagtaatt
     6841 aaactgatgt gtccctttac tggcctggcg atagataccg cttttgccag cagttgctga
     6901 ctttccggtg taaccgcaac agcgacaaga atattgctat agctcataac ctgttcctta
     6961 ttctgtgaac ttcaggtgac attaaagctt tactcctaat ggagaaacga cgcaatccca
     7021 actcggtcaa acaggtaaat attagtctga ttattagtaa agacaattaa ttaattactc
     7081 ccggtgatca ta
//
LOCUS       DAZGOB010000191         7079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGOB010000191 DAZGOB010000000
VERSION     DAZGOB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.191"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            112..1446
                     /gene="adeQ"
                     /locus_tag="ACHLIF_004667"
                     /gene_synonym="yicO"
     CDS             112..1446
                     /gene="adeQ"
                     /locus_tag="ACHLIF_004667"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO9608521.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1499..1951
                     /gene="yicN"
                     /locus_tag="ACHLIF_004668"
     CDS             1499..1951
                     /gene="yicN"
                     /locus_tag="ACHLIF_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO9608522.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2162..3352
                     /gene="nepI"
                     /locus_tag="ACHLIF_004669"
     CDS             2162..3352
                     /gene="nepI"
                     /locus_tag="ACHLIF_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO9608523.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3393..3686)
                     /gene="yicS"
                     /locus_tag="ACHLIF_004670"
     CDS             complement(3393..3686)
                     /gene="yicS"
                     /locus_tag="ACHLIF_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO9608524.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIHEAIVAMERNNQSNYWEALGKVECPDM"
     gene            3908..4726
                     /gene="nlpA"
                     /locus_tag="ACHLIF_004671"
     CDS             3908..4726
                     /gene="nlpA"
                     /locus_tag="ACHLIF_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO9608525.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4730..5653)
                     /gene="yicL"
                     /locus_tag="ACHLIF_004672"
     CDS             complement(4730..5653)
                     /gene="yicL"
                     /locus_tag="ACHLIF_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO9608526.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5764..6948)
                     /gene="setC"
                     /locus_tag="ACHLIF_004673"
     CDS             complement(5764..6948)
                     /gene="setC"
                     /locus_tag="ACHLIF_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO9608527.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLGDE
                     LKARPIMVDFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMICIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1778 a         1592 c         1784 g         1925 t
ORIGIN      
        1 attaaagaat atccattaat gtgcaattga aatgtgataa ttatcactga accgttgttg
       61 tcatttgttt tacaaaagca agggattaaa tctcctccaa tggacaaaaa aatgaataat
      121 gacaataccg attacgtgag taatgaatca gggacgcttt cgcgattatt taaactacct
      181 cagcatggga ccaccgtccg cacagaattg attgcgggga tgaccacttt tttaaccatg
      241 gtgtacatcg tttttgtgaa cccgcaaatc ctcggcgcgg cacaaatgga cccgaaagtg
      301 gtgtttgtta ccacctgttt gattgccggt atcggcagta ttgcgatggg gatatttgct
      361 aacttacccg tggcgctggc tccggcaatg gggctgaacg ccttctttgc cttcgtggtc
      421 gtgggggcga tgggcatctc ctggcagacc gggatgggcg caatattctg gggcgcagtt
      481 ggactatttt tgctcacgct gtttcgtatc cggtactgga tgatctccaa cattccctta
      541 agtttacgta ttggtatcac cagcggaatt ggattattta tcgccttaat gggattaaaa
      601 aatactggcg ttattgtcgc caataaagac acgctggtga tgattggcga tttaagttct
      661 cacggcgtgt tgttaggtat tttagggttt tttattataa ccgtgttgtc atcacgtcat
      721 tttcatgccg cggtgctggt ttctattgtg gtgacgtctt gctgtggatt atttttcggt
      781 gatgttcatt ttagcggcgt ctattccatt ccgcctgata ttagcggcgt cattggtgaa
      841 gtagatttga gcggcgcgtt aacacttgaa ctcgccggta tcattttctc ctttatgctg
      901 atcaacctat ttgattcatc aggaacatta attggtgtaa ctgataaagc gggcttaata
      961 gatagtaacg gtaaattccc caatatgaat aaggcgctgt atgttgatag cgtcagttcg
     1021 gtggcggggg cgtttatcgg cacctcgtct gttactgcct atattgaaag tacttctggt
     1081 gtggcagtcg gtggtcgcac ggggctgact gcggttgtgg ttggcgttat gttcctgtta
     1141 gttatgttct tctcaccgct ggtggcgatg gttcctcctt acgcaaccgc cggagcgtta
     1201 atctttgttg gcgtgctgat gacttcgagc ctggcgcgcg ttaactggga tgattttacc
     1261 gaatcggtgc ctgcgtttat taccacggtg atgatgccct ttactttctc gatcaccgaa
     1321 gggattgcac tcggctttat gtcgtactgc atcatgaaag tatgcaccgg gcgctggcgc
     1381 gatctgaacc tgtgtgtggt ggtggtcgca gctctgtttg cactgaagat tattctggtg
     1441 gattagtaac tggcgagggt ttccagatat catgagttct gattacgcag gagaactcat
     1501 gatctggata atgctcgcca cgctggcggt agtgtttgtg gttggttttc gggtgctgac
     1561 atccggggcc agaaaagcga ttcgccgtct cagcgatcgg ctgaacatcg atgtcgtacc
     1621 cgtggagtcg atggtcgatc aaatgggaaa gtcagctggt gacgaatttt tacgttattt
     1681 gcatcgtccg gatgagtcgc acctgcaaaa cgccgcgcag gtgttgctca tctggcaaat
     1741 tgtcattgtc gatggtagcg aacagaacct gctgcaatgg catcggattt tacaaaaagc
     1801 tcgcctcgcc gcgccgatta ccgacgctca ggtcaggctg gcgctaggtt ttctgcgcga
     1861 aaccgaacct gaaatgcagg atattaatgc ttttcagatg cgctataacg cgttctttca
     1921 gcctgccgag ggcgttcact ggttgcattg aaaccgtttc gtcttatcag cctacagacc
     1981 gcgcctgacc tgtcgggtgg ataagacgtt cacgccgcat ccggtaatct ttgccctgtt
     2041 ccgtttcgat cttaactcca gctcaagcaa caccgtaaaa gttgcaaaaa cgttaaatgc
     2101 gtcacacatt tcaatgttaa agtggtcggc ttttcccctg aaacatgcca cgggtaacac
     2161 catgagtgaa tttattgccg aaaaccgcgg cgcggatgcc atcacccgac cgaactggtc
     2221 agccgttttc tcggtggcgt tttgtgtcgc ctgtctgatt atcgttgagt ttttgcccgt
     2281 cagtttgttg acgccaatgg cccaggattt aggcatttcg gaaggggttg ccgggcaatc
     2341 ggtgaccgtg accgcctttg tggcaatgtt tgccagtttg tttattaccc agacaattca
     2401 ggctactgac cgccgctacg ttgttatttt gtttgccgtt ttgctgacgc tctcctgctt
     2461 gctggtttcc tttgctaact cattcagttt gcttttaatc ggtcgtgcct gtctggggct
     2521 ggcgctgggc gggttctggg cgatgtcggc gtcgctgacc atgcgtctgg tgccgccgcg
     2581 tacggtgccg aaggcgctgt cggtgatctt cggcgcggtt tctattgcgc tggtgattgc
     2641 cgcgccgttg ggcagttttt taggcgagct tatcggttgg cgcaatgtct ttaatgcggc
     2701 ggcggtgatg ggcgtgctgt gtattttctg gattatcaaa tcattgcctt cactgccagg
     2761 cgaaccctcg catcagaaac aaaatacttt ccgcttatta caacgtccgg gtgtgatggc
     2821 agggatgatc gccatcttca tgtctttcgc cgggcagttt gctttcttca catatattcg
     2881 cccggtgtat atgaacctgg cgggattcag cgtggatggc ttaacgctgg tgctgttgag
     2941 ttttggcatt gccagcttta tcggtacgtc gctttcgtca ttcattctta aacgttcggt
     3001 aaaactggcc ttagcaggcg cgccgttaat actggctgtg agtgcgttgg tgctgacgtt
     3061 gtggggaagc gataaaatcg ttgctaccgg cgtggcgatt atctgggggc taacttttgc
     3121 attggttccc gtcggctggt caacgtggat cacccgctcg ctggccgatc aggcagaaaa
     3181 agccgggtct attcaggtgg cggttattca gcttgctaat acctgtggcg cggcaatcgg
     3241 cggttatgcg ctggataata ttggtctgac ttcgccgttg atgttgtccg gcacattgat
     3301 gttgctgact gcattgttgg ttactgcaaa ggtgaaaatg aagaaatcct gatatcaggt
     3361 aaagaagcgt tggtcagtag ttgttgctgt tcttacatat ccgggcattc taccttaccc
     3421 aacgcttccc aataattgct ctgattatta cgttccatcg ccacgatggc ctcgtgtata
     3481 tgttgtttat tgttttcatc tgacaacatt aacttttccc acgcttcatc tgataatgac
     3541 gcctgtggcg tgcaaatttt acgtaaatct tgtaataccg tggttttctc ttttgcggac
     3601 tgcagtgagc taaaaggcga ctcggcgtat acgatgcctg gcatagcgaa aaaagtgaaa
     3661 ataagcagta gtgtcgttgg tttcatggat tcaacctttt acaaatgtgt atcgaatgaa
     3721 gcataagtgt agtttgcttt catcgctctc atcatgctgt acgaaacgcg cttgcgtggt
     3781 cagtaagaag tgccagactt tatattccac ttttattcct ttttattctt atcgatagcg
     3841 tttcgttttt taaaccgcag cgaccttacc gctatagtca ggtaatcatt aataaaagga
     3901 taaaaaaatg aaactgacaa cacatcatct acggacaggg gccgcattat tgctggccgg
     3961 aattctgctg gcaggttgcg accagagtag cagcgatgca aaacacatta aagttggcgt
     4021 tataaatggc gcagaacaag atgtcgcgga agtcgctaaa aaggtggcaa aagagaaata
     4081 tggcctcgat gttgaactgg taggatttag cggttcgttg ctaccgaacg atgcgacaaa
     4141 tcatggcgaa cttgatgcga atgtcttcca gcatcgaccc tttcttgaac aagataatca
     4201 ggcgcatggt tataaactgg tagcggtggg aaatacgttt gtcttcccta tggcgggtta
     4261 ttccaaaaaa atcaaaaccg tggcgcaaat aaaagaaggc gcgacagtgg cgatccccaa
     4321 tgacccgacc aaccttggtc gcgcactttt actgttgcaa aaagagaagc taattactct
     4381 gaaagaggga aaagggttat tacctaccgc gctggatata accgataatc ctcgtcattt
     4441 gcagattatg gaactcgagg gggcgcagct cccgcgcgta ctggatgatc ccaaagttga
     4501 tgtagcgatt atcagcacca cttacattca gcagacaggg ctttctccgg tgcacgacag
     4561 cgtatttatt gaagataaga attcgccgta tgtgaatatt ttggtggcac gggaagataa
     4621 taagaatgca gaaaacgtga aggaatttct gcaatcttat caatcacccg aagtcgctaa
     4681 agcggcagaa accattttta acggtggcgc ggtgcctggc tggtaattct cactcatgcc
     4741 gcgccggacg atttattttt ctggcgcggc gacgggaatc cattgaaatc aaaattaccg
     4801 atgacagaat tagcagcgtt cccagccagt ccggtaatgt gaacgtgatc cccagcaaca
     4861 gcaaagagag tagcgcgcta cttaatggtt ctgcacagct caaaatgctg gcttttggac
     4921 cgccaattaa ttgtgctcct ttcaggtaca gactaaatgt cagggacgta ccaatgacga
     4981 tcaaataaaa aaacgccaga atcaaactgc cgttaacgac aaagtttgtt ccttgtctgg
     5041 cataaaaagg caacagaatc agaccgccaa tcagcatact ccagccgacg actggtaacg
     5101 tgccatagcg ggcaattagt gttgagggat aggtggtata gaatgcagca gcaaatgccg
     5161 aggcaatgcc ccagaacaac gcggcaggag agatcgataa tgacgtcgga ttaccgtgtg
     5221 tcaccaataa aaaagtcccg accagcgatg tcaaaatagc gcagaaaacc agaatgcccg
     5281 ggcgcgattt acgcaccagt gagaaccagg cgacgataat cgtcggtgag aggaattgca
     5341 gcaccgttgc cgtggctgcg ttcgattttt cgatggttag caaaaaagtg agctgtacag
     5401 ttagcgcgcc aaccacggaa aaaatcagca ggctaatggc atctttatga ttgttaataa
     5461 tagaaaagat tttatcgcca tgaacaaatg acagcgtcag tagaatcaaa ccggcgaata
     5521 tcaaacgcgt catagtcaaa aactgcgacg acatctggct ttgctccatg atgtattgcg
     5581 cacagacccc tgaacttccc cacaacacgg cggcaatcag aacgttcagc atcccctttc
     5641 tggtggaacc catttttccc tctgcatact gttttgtcgt ttatgtatgc aggcatgata
     5701 gcaaaaatgg cgaggatggc tctgtttgcg ccacgccgat ttttcttagc ccagactggt
     5761 gagctaaata tctttaataa acagcaggca aatcatcgca atacccaaca tccctatcgc
     5821 cagccagaac aacgcatggt agctccagat ttcaaccata attccgtcaa cagagccggc
     5881 gataatccag ccgacgcgtg aagtatttgc atataacgtt gtcgcagagc ctattttttc
     5941 aggcatcaag tcctgaaaat aaagcatgcc gataccacag agaataccaa ggaagatggc
     6001 attaagaatt tgcaattcca gctcaaccgc cggagtcgtc gccatgagta cgctggcgta
     6061 aaaacacatt ccactcacga tagcaatgag cattaatagt cgcttgccaa tacgtttcat
     6121 gtaatagcct gcgattaaca tcatcggaat ttccagaccg gcagcgatac aaatcatttc
     6181 tccagccagt ttatcggtta gatgcagttc atcaataata aatagcggca tatttatcat
     6241 gtagagatta ttcgccgccc acatcattga acagaccaca aaaagtagcc gcgtatcccg
     6301 cttcctgtgg gtggagggta aaatttctac gggttgggta acgacaggaa tatttctttg
     6361 tattgatggc aaaaacaacc agactatcag tccgcaaaca acaaatgcga tggcagcggt
     6421 gagatacatc actttaaaac taaatcccat tgccagttca taagcgagcg gtggcccgat
     6481 aacccaggca agcgagatct gagcacgtaa aaatgtactg aacatgaccg tctcacggcc
     6541 tgttctgtcg gcgtgttcac gggcgagggc gaacatttgc gggtttgcgg tggaagcaaa
     6601 actactcaga agtacgcccg ttgagaggag aatgaagtag ttgcgattcc acgcaaaaag
     6661 cgtgcaggcc agcactccaa ataagcaaca tagcagaatc agtaatttac ggtcgccttg
     6721 tttatcggag tgccttgcca gaaattgact gaccagaatt cccataatag cgctaccggt
     6781 gaagaaaaaa tctaccatta taggacgggc tttcagttca tctccgagga atatacttag
     6841 ggtaggagtc tgaagagcgc ccgcaatacc cgtcagaaag gcgacaagta aaaatgccgc
     6901 ggcagtgaga tcaagtattt ttgatggagt ggtagccgtt ttttgcattc ttctgaatca
     6961 ccgggcgaca aaatataact gagttacaaa taagtaccag tcgaatgagt attgtattaa
     7021 gaatttttga gtatcgctga tacaaccaga aagcaatgct agcgcaatat tatagggcc
//
LOCUS       DAZGOB010000192         6870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGOB010000192 DAZGOB010000000
VERSION     DAZGOB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.192"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..456
                     /locus_tag="ACHLIF_004674"
     CDS             <1..456
                     /locus_tag="ACHLIF_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO9608528.1"
                     /translation="DLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVI
                     CTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEE
                     NIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(610..2259)
                     /locus_tag="ACHLIF_004675"
     CDS             complement(610..2259)
                     /locus_tag="ACHLIF_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO9608529.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2411..3760)
                     /gene="ghxP"
                     /locus_tag="ACHLIF_004676"
                     /gene_synonym="yjcD"
     CDS             complement(2411..3760)
                     /gene="ghxP"
                     /locus_tag="ACHLIF_004676"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO9608530.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4306..4770)
                     /gene="soxR"
                     /locus_tag="ACHLIF_004677"
     CDS             complement(4306..4770)
                     /gene="soxR"
                     /locus_tag="ACHLIF_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO9608531.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4856..5179
                     /gene="soxS"
                     /locus_tag="ACHLIF_004678"
     CDS             4856..5179
                     /gene="soxS"
                     /locus_tag="ACHLIF_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO9608532.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5182..6768)
                     /gene="pdeC"
                     /locus_tag="ACHLIF_004679"
     CDS             complement(5182..6768)
                     /gene="pdeC"
                     /locus_tag="ACHLIF_004679"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO9608533.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGCFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
BASE COUNT         1905 a         1817 c         1595 g         1553 t
ORIGIN      
        1 gatttaatta actttgaaca tgctgatctg tctcgcgtca atctgaacaa agcaatcctc
       61 cagaatataa acttaattga tagcaaactc ttttgtacgt ggctaacaaa tacattcctc
      121 gaaatggtta tatgtaccgg ctctaatatg gctaatgtta attttaataa tgccaattta
      181 agcaactgcc atttcaactg ttctatttta acaaaagcct gtatgtttaa tacccgtctc
      241 tatcgggtta attttgatga ggctagcgtc cagggaatgg gcatttctat tctccgtggg
      301 gaggaaaata tccccattga tagtgatacc ctggtaacac tacagaaatt ctttgaagaa
      361 gattgtacct ctcatactgg catgtcacaa actgaggata atattaatgc agtcgctatg
      421 aagattactg cagatattat gcaacacgca gattgaggtt aagtcgaaga acagccaatg
      481 aattgacttt acaagagaga taacttattt gttttagaat catgaatgtt agcccgagcc
      541 aacctgttaa aggtgcaaaa gattgcacct ttttcagatt acctcgggcc tggttacgtc
      601 gctttcggct tactgatttt cctcaattag caacgcttca agcaaatcga gatcgtgcag
      661 taatttttgc agcgtttcgt tgctgatctc ccgcgtggcg cgcaggtggt acagttcagc
      721 acgttcagaa cgcaatgccg ccagacggaa gcgacgctca aggttctctt cctgcacgga
      781 actttcaacg tcgttacgtc catcggcgcg acgacgcagg ttaccaatga cgcgagaact
      841 gacctccgta agcagctggt tatcgatatt ctcttcggta tccgccgcca gacgctcctc
      901 cattttctgg atcgccacaa tcgccacttc tgccgttgcc gctcgcgcaa tacgttcttc
      961 tttcaattgt tgcgaatggt ccgcgacttc aatgtgttgt agcagaatag gcaacatcac
     1021 cacgccgaca aacagcgaga agagaatgac gccagccgcc aggaacacca gctcatagcg
     1081 cgccgggaag acgttaccat ccggcaagag cagcgggata gagagcacac cggccagagt
     1141 gatcgcccca cgcaccccgg cgaaagacgc gatcaggatt tctcgtgtgg tccacgaacc
     1201 aaactccatc ggcttcttct tcaggaagcg gttgctgaac tttttcatcg tccacagcca
     1261 gccgaaacgg accagcatca gcgccgcata tatcagaata atatcggtaa acagcatcca
     1321 gatttcgacg ttagggtcga tttctgccgc catcagcgac gtctccagaa tacccggcag
     1381 ctgcagacct aacagcagga acaccatgcc gttaaagaca aattccagca tcgcccaggt
     1441 gctgtttgcg cgcaggcgca ttgccagcgg cgcacggcgc atcacaccgg agcgggtgat
     1501 ggtcatcccg gcggcaaccg cagcgaggat gccggaaacg ccaatatgtt cggcaatcag
     1561 ataagaagcg aatggcagca agaacagcag aacgatctgc gtcgcgggtt catcaccgcc
     1621 ccagcggctg aggaatcgca gcgagcgacc atacagccag ctcaccacaa agccggcgag
     1681 aataccgcca atggctactt tcataaattc gaccgtcgca ccgccaacgg tgaagatcat
     1741 cgtccccatc gccactgcca cggcaaactt caacgacacc aggccggagg cgtcattcat
     1801 caacgcttcg ccctgcaaaa tacccatgat ttttttcggg atgcgccctt cgccaacaat
     1861 cccggagagc gccacagcat cggtcggaga aagcactgcc gccagcgcaa aggcggggat
     1921 cagcggaatg cccggcacca cccagtaaat aaggaagcca atgccgacca cggtgaccac
     1981 caccagcgcc agcgcgaggc caaaaatctc tcgaccatgt tcgagaaatt cacgggtcgg
     2041 cgttttccag ccatcagcga acagcaacgg cgggataaac aagactaaaa agagttcagg
     2101 atcaaactcc acatgcaaac caaacgtcgg ccacgccagt agcgcaccga tggcgatttg
     2161 cataagcgga agcgggatct gaaagggcat gacacgagtg accaccccgg acagcgagac
     2221 cacgagggtc attatcagta tggtgaagaa gatttccatg cgttccctgt ttgcgatgtt
     2281 tttttatgat ctgagaaaga cgttatgcct gaattctcat tttttcagaa tatcgcaaaa
     2341 gctcacacaa tgtgcggaga aaataaaaac gggcagccat tgccacccgt tttcagaatc
     2401 cgatgaaaga ttagatagcc cagccacccg catagaaggt caccagcgcc acggcgataa
     2461 cgaccgtacc gatgttcaac ttgcgccatt cgccggaaac cagacgacca atcaccagag
     2521 tcgcgaagcc gatcatgatg cctgttacga tgttacaggt cagcacgatg aataccgccg
     2581 taaccagacc cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc acgttgctca
     2641 gcatcagcag gccaacgtac atcagcgccg gagccgttgc ataccccgga acgaggtaag
     2701 agagcggaga gaggaacaga atcagcagga acagcacgcc aacggtgata gccgtcaaac
     2761 cggttttacc gcccgccgcc gtacccgccg cagactcgat gtataccgct gccggagcag
     2821 cacccaccag gccagagaaa acgctgctca tggagtcagt ggtcagtgct ttcccaccgt
     2881 cgatgatctg cccatcttta tccagcaggt tcgcctggcc ggcgacggca cggatagttc
     2941 cggtggcatc aaatactgcc gtcatcacca gcgccagaac gcttggcagg actacaggat
     3001 tcagcgcgcc cataatgtcc aggctgccaa tcagtgaatt gccgttttca tcgctcaatg
     3061 aaggcatggc gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa ccgacaattg
     3121 agataccgat aatggtcagc agaatgccac cagggacttt cagtttttcc aggccgatga
     3181 tcaccgccag acctactagt gacataatca ccgggaaggt cgcgaaatca cccagcgcaa
     3241 cgggcagacc atcaagcggg tttttaatca ccagaccgac accattagcg gcaatgagca
     3301 gcaggaacag accgataccg atccccgtgc cgtgcgccac accgtgcggc aagttgcgca
     3361 aaatccagct acggataccc gtggcagaaa ttaccgtaaa cagcaccccc atcaggaaca
     3421 cggcacccag cgcgacaggt acgctaatat gttgccccag caccaggctg aatgcggtaa
     3481 acgccgtcag ggagatggcg caaccaatcg ccaacggcag attagcccac agacccatca
     3541 cgatagaacc gagtccggca accagacagg ttgcaacgaa aactgccgca ggcgggaagc
     3601 ctgctttacc caacatacct ggaacgacga tgaccgagta gaccatcgcc agaaacgttg
     3661 ttaacccggc aaccacttcc tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg
     3721 cgtcgagtga accgccggta cgcgctgatg gcgtagacat aagaaaacat cccctgagag
     3781 tttaattttc gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt tatcttcctg
     3841 ctccaggttt gcgacaattt actgcgtcgc aaaaaagcaa acgtttagct ccgcgcatac
     3901 aaaacgggtg gtaaaaaaag caaacgatta tccggcgatc atagcggctt tttcaaccga
     3961 agtttgctgc ttttttctct tttattgtct tgacgatgaa aaaccaggca attgatgcgc
     4021 aaacgttatc gtcggtgcgc aagattgcag cgttatcgag ccgtataccg ttcaggattg
     4081 cgataaaaaa tcgtctgaga tgacaaaaat attgcggata aaaagtatac ttttacactg
     4141 gcgaagaaat gccggtgaca tacaattaaa gcatcaacac caaccggaac ctccaccacg
     4201 tgctcgaatg gggtgtgttg acgtcggggg aaaccctcct gtgtaccagc gggatagaga
     4261 gaaagacaaa gaccggaaaa caaactaaag cgcccttgtg gcgctttagt tttgttcatc
     4321 ttccagcaag cgtgcgccgg taccttcttc tcctaagcgg tcgcccgggt tacgcaacgg
     4381 gcaatcactg cgcgaaaggc agccacaacc aatacatccg tccagttcgt cacgcagcgc
     4441 tactaaggta tgaatgcgcc gatccaactc ttctcgccat tgggacgaaa gctgtttcca
     4501 ctctttcgca cttaacgtat gcccttcggg caacacgcca aacgcttcac caatggtcgc
     4561 cagcggaatg ccaatacgct gagcaatttt gataattgca acatatcgca acacatcacg
     4621 tttatatcgc cgctgattgc cgctgttacg gatactggta atcaaccctt tactttcata
     4681 gaaatgcagc gccgataccg ccacaccgct gcgtttcgcc acttcgccgg gggttagcag
     4741 cgctttaatg cggggtaatt tcttttccat aaatcgcttt acctcaagtt aacttgagga
     4801 attatactcc ccaacagatg aattaacgaa ctgaacactg aaaagaggca gatttatgtc
     4861 ccatcagaaa attattcagg atcttatcgc atggattgac gagcatattg accagccgct
     4921 taacattgat gtagtcgcaa aaaaatcagg ctattcaaag tggtacttgc aacgaatgtt
     4981 ccgcacggtg acgcatcaga cgcttggcga ttacattcgc caacgtcgcc tgttactggc
     5041 cgccgttgag ttgcgcacca ccgagcgtcc gatttttgat atcgcaatgg acctgggtta
     5101 tgtctcgcag cagaccttct cccgcgtttt ccgtcggcag tttgatcgca ctcccagcga
     5161 ttatcgccac cgcctgtagt tttattgccc gcgcgttaac tcccgcgcgg gtaattgctc
     5221 catccattgc ataaacacct gcggcggcat cgccttcgca aaaaaacatc cctggcaata
     5281 gcgcacgccg cgtttgcgca gccagttaac ctgctcctca gtttcgacgc cttcagcgat
     5341 cgtttttaac cccaggctgt gcgccagctc gatgatgtgt tccgcaatca aatgactggt
     5401 tttgtgggtg gtcagcgttt caacaaacga tttatcgatt ttcaaaatat cgacattcaa
     5461 tgatttaagg ttatgcaagt tagagtagcc aataccaaaa tcatcaattg ccacttcgta
     5521 accagcctgg cggaaagcca gaataatcgg cgtcattttg tcaacatcaa gaaatgcatg
     5581 ttcagtcact tcaaatttaa tttgctgcgg acgcaccgcg tattgctctg ttttctgatt
     5641 gattcgcgct atcaaccgtg acgtatgaaa atcggaggcc gacaggttaa tagaaacata
     5701 gcgatcggca tgtgttgcca agtacgcgcc cagatcacgg aagacattat caataacata
     5761 atcagttatc tgttctatca tcccctcctt ttctgccagc ggaataaact ctgccggatt
     5821 cattatttgc ccctgctcac caagccaacg taacaacgct tcagcgccga tacatttttc
     5881 tgttttgata tcgattattg gctggtaata aagacaaagt tgatgttttt cgagggcgcg
     5941 ttgcaattta cgtttgggag ataaatagtt ttgtcgaata cgtagccaga gtaatagcag
     6001 aacaagactc cccaaaatac cggcgggcaa cgcaaaaata agatgattat aaaaatgggt
     6061 tataagacgt tgatatgaag tcgcaacaat ggctgcaatt gggcgttttg tcgaataaac
     6121 tgtcgcatat aaatagccat ttctttgtac ggttaaatcc ttcaaatgaa tcagcggaga
     6181 aaacgttgct gccgaggcct ctttgcttaa cgagaaaaag gttttcgtca ccgtatcata
     6241 cacaccccat tgcaatgtcg gatcatcaga catcacttca ctccagaaga gagggttgat
     6301 aaccgccaca taatttcccc gctgcatata ggtcatttta tagccagaga aaaaaggcgt
     6361 atcgcggtaa taatagatag aaacgttagg ttcacgctta taatcggccg gtgcaatcgt
     6421 atagccgttt acaggcgcta tcagcgatga gcataaaaaa tggttatcac gggcatagat
     6481 caattcatta atataaagat agccacgaat aatattcaac attcgctttt gatgggctgg
     6541 agtgcaaact tgcccctgat agcgttcagc tgcatcgctc actaaatctg cctggcgaat
     6601 gaccagctcg gatttatcca gtgccagttg agcaaaggtt cggagctgat tattcacttc
     6661 tgattttgcc caaaggaagg caatccatag cgaaagaatg atgggaaaga gaactaaaaa
     6721 gataatgccc ggcaacgcca gtaattggtg tcgtgcacga tgactcattg ctatcaattt
     6781 cctgcctgtt tatacccggc cattgcaagg tgaaaaccct gggtaccgac gtctttagat
     6841 aaatcttacg gtataaatag cacttttgcc
//
LOCUS       DAZGOB010000193         6827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGOB010000193 DAZGOB010000000
VERSION     DAZGOB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.193"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHLIF_004680"
     CDS             complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHLIF_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO9608534.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHLIF_004681"
     CDS             complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHLIF_004681"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO9608535.1"
                     /translation="MKRRDTILRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHLIF_004682"
     CDS             complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHLIF_004682"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO9608536.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHLIF_004683"
     CDS             complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHLIF_004683"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5114..5116),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO9608537.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHLIF_004684"
     CDS             5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHLIF_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO9608538.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1496 a         1840 c         1843 g         1648 t
ORIGIN      
        1 atacgcttgt catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag
       61 gcaaacagac aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatggtgtc
      121 gtcacaccct atgagctgta atccgggcga ccagcgccac ccggaaaacg tcagagatta
      181 ctccccttca cccggaaaca ggaacgggtt gatggaactg cgggcatagc cttcttgctc
      241 cattcgcgcg tccagtacca gagaggcgag gtcatctgcc acggcttcaa ctttcggttc
      301 tttttcctga taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat
      361 cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct
      421 gcattttacg cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag
      481 accctgagtg gtgccaattt gcaccatgct ggacaccggc atagaaccac aaaccgggca
      541 atattgacgt tgctcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg
      601 cgcccagtag agcgacagtg cagcccagat aaacggcgct ttatcgctgc tgacggatga
      661 gaaatcagag gcaaacagtg cgctggccat atcttccagc tcctgagtcg atgccttctc
      721 cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaatcag
      781 cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg
      841 cttgccctgt gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc
      901 gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag
      961 cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag
     1021 ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg aacccagctc
     1081 atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca
     1141 cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg
     1201 gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt
     1261 aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa
     1321 gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa
     1381 ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact
     1441 tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt
     1501 aggttgtgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg
     1561 acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg
     1621 aaggacggga acaaaaagcc cagcccgctc accgccgcca ggatgaagca gaaggcggtg
     1681 atccagtggt tgatacgttc cggcgcggtg tagcgcagga tggtgtcacg tcgtttcatt
     1741 tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac
     1801 cgacacccac gtagtggaag atactggccg caaaggtagc cgcaaagcca acagccgcga
     1861 gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca
     1921 agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacattacg tgtgtaccac
     1981 cgacgcctgc cggatcgtac agacccgcat tgtcgtaacc gcgggttttc agctcagcaa
     2041 cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc gcgcccgttg
     2101 ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg cacagcgtac
     2161 atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa
     2221 tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact
     2281 gaataattgc accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac
     2341 agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca
     2401 ttaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc
     2461 cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga
     2521 tacaggtggt gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctgaggcg
     2581 cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat
     2641 gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg
     2701 tgttcgcatc accaacgaat ggcgttaacg tattggcaat aaagcctttt ttagctacgc
     2761 cttcatagcc ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca
     2821 gtgtacgaat acgtttggtc accaccgctt tggccttgat atagccacgg ttggaggaga
     2881 ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca
     2941 caaactgctc tggttgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaaat
     3001 gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt
     3061 cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga tgcagcgggt
     3121 tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct
     3181 tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc
     3241 tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc
     3301 atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt
     3361 tatacagaat gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc
     3421 cagacggatc ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg
     3481 cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa
     3541 gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg
     3601 ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt
     3661 tccaggtcat gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc
     3721 gcaagcgcag gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt
     3781 ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt
     3841 ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt
     3901 cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagc gggtcgatgg
     3961 tgacgaggaa cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg
     4021 caacagggtt aaagccctgg cagatatagc cattgacctt gccttctttc atcatctcga
     4081 agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat
     4141 tttccgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt
     4201 agttgcccca gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt
     4261 aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg
     4321 acagtagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc
     4381 cgccgccggc catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt
     4441 tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat
     4501 cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac
     4561 cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc
     4621 acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt
     4681 cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc
     4741 catcttcaaa gccgtaatcc tcacgcacta tcaggctggc gttggtatag gcttcggtgt
     4801 attcgcggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa
     4861 tgtcagtacc ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc
     4921 gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga
     4981 acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct
     5041 tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta
     5101 ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg
     5161 agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg
     5221 tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc
     5281 ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg atttgctgcc
     5341 atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga
     5401 aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat
     5461 cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca
     5521 tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca
     5581 gcagtttata ctgccgggtt tccgcgagcg ctacgctggg tgcaaaaccc agtgccgctg
     5641 ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca
     5701 tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcgtgtgca
     5761 gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt
     5821 tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt gtgttcttat
     5881 ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat cgaaaatgtg acaaatatca
     5941 caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca cggttagatg
     6001 aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga
     6061 tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggataa
     6121 tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag
     6181 tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg
     6241 cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg
     6301 tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc
     6361 atctcaacga ttttcagcca gtggggcaac tgactggttg tactcacgcc gctgcctgga
     6421 tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg
     6481 acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg
     6541 tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt
     6601 tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgt tgtaatctga
     6661 cgctggtggg tttttgtaag ccgggtcggg caacggttta tacccatccg cagcgtttga
     6721 gcaattaatt aaaataagtc ctgcgatata tattaaattg caggacttat tcatttcgtg
     6781 aattttatta ttttatttat aaataaaacg cgtgtagtgg cgttttc
//
LOCUS       DAZGOB010000194         6782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGOB010000194 DAZGOB010000000
VERSION     DAZGOB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.194"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            300..581
                     /gene="yjcB"
                     /locus_tag="ACHLIF_004685"
     CDS             300..581
                     /gene="yjcB"
                     /locus_tag="ACHLIF_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO9608539.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            complement(680..1216)
                     /gene="ssb1"
                     /locus_tag="ACHLIF_004686"
     CDS             complement(680..1216)
                     /gene="ssb1"
                     /locus_tag="ACHLIF_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO9608540.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            1470..4292
                     /gene="uvrA"
                     /locus_tag="ACHLIF_004687"
     CDS             1470..4292
                     /gene="uvrA"
                     /locus_tag="ACHLIF_004687"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO9608541.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(4327..4683)
                     /gene="yjbR"
                     /locus_tag="ACHLIF_004688"
     CDS             complement(4327..4683)
                     /gene="yjbR"
                     /locus_tag="ACHLIF_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO9608542.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4687..5103)
                     /gene="yjbQ"
                     /locus_tag="ACHLIF_004689"
     CDS             complement(4687..5103)
                     /gene="yjbQ"
                     /locus_tag="ACHLIF_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO9608543.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(5214..5927)
                     /gene="aphA"
                     /locus_tag="ACHLIF_004690"
     CDS             complement(5214..5927)
                     /gene="aphA"
                     /locus_tag="ACHLIF_004690"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO9608544.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKLGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            6329..6551
                     /locus_tag="ACHLIF_004691"
                     /pseudo
     CDS             6329..6551
                     /locus_tag="ACHLIF_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6581..>6782
                     /locus_tag="ACHLIF_004692"
     CDS             6581..>6782
                     /locus_tag="ACHLIF_004692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608545.1"
                     /translation="MYICTLCCFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKELS
                     LTLEFNDTIIESQKVTGNKAKHI"
BASE COUNT         1690 a         1696 c         1705 g         1691 t
ORIGIN      
        1 tcacctggat gaaaataatt cattgatatt caaaattaat ttcctacagg aatgcagagg
       61 cggcgggaag atatgccctt aatgccactg aaaaaccaca taatctgtac agcaataaaa
      121 gtcacggcct aatatggcta acaaacaatc aattgtgata tagttcacaa aattaatgaa
      181 acaaacagag tgtttcattt ttgtgttctg caagccccgc gatagccaga gtgtaccacg
      241 cctcccgtga acaacgccgc gctgtccagg gcatcggctc ttttcagagg aattgtttga
      301 tggctaccct caccactggc gtggttcttc ttcgctggca acttcttagt gccgtaatga
      361 tgtttctggc cagcacactc aacatccgtt ttcgtcggtc tgattatgtc gggcttgcgg
      421 tgatcagcag cggtctgggc gttgtttccg cctgttggtt cgcaatgggg ttgcttggca
      481 tcacaatggc ggacatcacc gctatctggc acaacatcga gttggtgatg atagaagaga
      541 tgaatcagac accgccacaa tggccaatga ttttgacctg atacgtagaa gcctccataa
      601 cggaggcttc ttttttatgg ctggcagatg ctttaatcat ccaccttaaa acaatataac
      661 ctattgtttt aatgacaaat cagaacggaa tgtcatcatc aaagtccatc ggcggctcgt
      721 tagacggcgc tgccggagcg gactgctgcg ggcgagactg cgcgccgccg ctgaactgat
      781 tgccaccctg cggctgctgt ggctgacccc aaccgccctg cggctgacca ccaccgatat
      841 tgccacctgc cggagcgcca ccaccctgac gaccacccag catctgcatg gtgccgccaa
      901 cgttcaccac gacttctgtg gtgtagcgat cctgaccgga ttgatcggtc catttacggg
      961 tacgcagctg accttcgata taaacctgag aacctttacg cagatattcg ctcgccactt
     1021 ctgccagttt gccgaacagc acaacgcggt gccattcagt ctgttctttc atctcgccgg
     1081 tcgctttatc acgccaggat tcggaagtag ccagcgtaat gttggcaact gcgccaccat
     1141 ttggcatgta gcgtacttcc gggtcctgac ccagattacc aacgagaata accttgttta
     1201 cgcctctgct ggccatgttc gtgtctcctg aaaaaaatcg ttctgaataa gtgtaaacgc
     1261 gcgattgtac cattaccaat agcgctttta ctatgttgtg acctcggttc cgggaaacaa
     1321 acctggccag acattgttac acaacactcc gggtaatgca ttccaatact gtatattcat
     1381 tcaggtcaat ttgtgtcata attaaccgtt tgtgatcgcc ggtagcacca tgccaccggg
     1441 caaaaaagcg tttaatccgg gaaaggtgaa tggataagat cgaagttcgg ggcgcccgca
     1501 cccataatct caaaaacatc aacctcgtta tcccccgcga caagctcatt gtcgtgaccg
     1561 ggctttcggg ttctggcaaa tcctcgctcg ctttcgacac cttatatgcc gaagggcagc
     1621 gccgttacgt tgaatccctt tccgcctacg cgcggcagtt tctgtcactg atggaaaagc
     1681 cggacgtcga tcatattgag gggctttctc ctgccatctc aattgagcag aaatcgacgt
     1741 ctcataaccc gcgttctacg gtggggacaa tcaccgaaat ccacgactat ttgcgtttgt
     1801 tgttcgcccg cgtcggcgaa ccgcgctgtc cggaccacga cgtcccgctg gcggcgcaaa
     1861 ccgtcagcca gatggtggat aacgtgctgt cgcagccgga aggcaagcgt ctgatgctgc
     1921 tcgcgccaat cattaaagag cgcaaaggcg aacacaccaa aacgctggag aacctggcaa
     1981 gccagggtta catccgtgct cgtattgatg gcgaagtctg cgatctttcc gatccgccga
     2041 aactggaact gcaaaagaaa cataccattg aagtggtggt tgatcgcttc aaggtgcgtg
     2101 acgatcttac ccaacgtctt gcggagtcgt ttgaaaccgc gctggagctt tccggtggta
     2161 cagcggtagt ggcggatatg gacgacccga aagcggaaga gctgctgttc tccgccaact
     2221 tcgcctgccc aatttgcggc tacagtatgc gtgaactgga gccgcgactg ttttcgttta
     2281 acaacccggc aggtgcctgc ccgacctgtg acggccttgg cgtacagcaa tatttcgatc
     2341 ctgaccgcgt gatccaaaac cccgagctgt cactggctgg cggtgcgatc cgtggctggg
     2401 atcgccgcaa cttctattac ttccagatgc tgaaatcgct ggcagatcac tataagttcg
     2461 acgtcgaagc gccgtggggc agcctgagcg cgaacgtgca taaagtggtg ttgtacggtt
     2521 ctggcaaaga aaacattgaa ttcaaataca tgaacgatcg tggcgatacc tccatccgtc
     2581 gtcatccgtt cgaaggcgtg ctgcacaata tggagcgccg ttataaagag acagaatcca
     2641 gtgcggtacg tgaagaatta gccaagttta tcagcaatcg cccatgcgcc agctgcgaag
     2701 ggacgcgtct gcgtcgggaa gcgcgccacg tttatgtcga gaatacgccg ctgcctgcta
     2761 tctccgacat gagcatcggt catgcgatgg aattcttcaa caatctcaaa ctcgctggtc
     2821 aacgggcgaa gattgcggaa aaaatcctta aagagatcgg cgatcgtttg aaattcctcg
     2881 ttaacgtcgg cctgaattac ctgacgcttt cccgctcggc agaaacgctt tccggcggtg
     2941 aagcccagcg tatccgtctg gcgagccaga ttggtgcggg cctggttggc gttatgtacg
     3001 tgctggacga gccatctatc ggcctgcacc agcgcgataa cgagcgcctg ttgggtacgc
     3061 ttatccatct gcgcgatctc ggtaataccg tgattgtggt ggagcacgac gaagacgcaa
     3121 ttcgcgccgc tgaccatgtg atcgacattg gcccgggcgc aggtgttcac ggcggtgaag
     3181 tggtcgcaga aggtccgctg gaagcgatta tggcggtgcc ggagtcgttg accgggcagt
     3241 acatgagcgg caaacgcaag attgaagtgc cgaagaaacg cgttccggcg aatccggaaa
     3301 aagtgctgaa gctgacaggc gcacgcggca acaacctgaa ggacgtgacg ctgacgctgc
     3361 cggtgggtct gtttacctgc atcaccgggg tttcaggttc cggtaaatcg acgctgatta
     3421 acgacacact gttcccgatt gcccaacgcc agttgaatgg ggcgaccatc gccgaaccag
     3481 caccgtatcg cgatattcag gggctggagc atttcgataa agtgatcgat atcgaccaaa
     3541 gcccaattgg tcgtactcca cgttctaacc cggcgaccta taccggcgtg tttacgcctg
     3601 tgcgcgaact gtttgcgggc gtaccggaat cccgtgcgcg tggttatacg ccaggacgtt
     3661 tcagctttaa cgtccgtggc gggcgctgcg aagcctgtca gggcgacggt gtgatcaaag
     3721 tagagatgca cttcctgccg gatatctacg tgccgtgcga tcagtgtaaa ggtaaacgct
     3781 ataaccgtga aacgctggaa attaagtaca aaggcaaaac catccacgaa gtgctggata
     3841 tgaccatcga agaggcgcgt gagttctttg atgccgtacc tgcactggcg cgtaagctgc
     3901 aaacgttgat ggacgttggc ctgacgtaca ttcgcctggg gcagtccgca accacacttt
     3961 ctggtggtga agcccagcgc gtgaagctgg cgcgtgagct gtcaaaacgc ggcaccgggc
     4021 agacgctgta tattctcgac gagccgacca ccggtctgca cttcgccgat attcagcaac
     4081 tgctcgacgt actgcataaa ctgcgcgatc agggcaacac cattgtggtg attgagcaca
     4141 atctcgacgt gatcaaaacc gctgactgga ttgtcgacct gggaccagaa ggcggcagtg
     4201 gcggcggcga aatcctcgtc tccggtacgc cagaaaccgt cgcggagtgc gaagcttcgc
     4261 atacggcacg cttcctcaag ccgatgctgt aatcgttaag gccgctttct gagcggcctt
     4321 ttcctttcag agttgtacca gcaatttacg cttttcttcc ggtagtaaat tcactgcctg
     4381 ctgataagac gcatccacca gataatagat ttgcgaatcc ggcagcgaac cgtcgagata
     4441 cacggtgctc cagtgcgctt tattcagatg gcggctagga cgcacatcgc tgtgctgctg
     4501 acgtagcagc tccgccagct ccgggctggt tttcagcgaa acagctgggc gattttctac
     4561 ttctttcacc atcgcaaaca gtacatcttc cactttgatc tgcgtcgctt tccagtcgtt
     4621 atgcacgctc tgttctgcgc ctggttttgc catgcaatat tgtagcaact ccgaaatggt
     4681 cattttttac tccccttgta gtgtcgcgat gatgcgacgc gatccgccgt ggatgcgatg
     4741 ttcccccagc cagatgcctt gccaggtgcc ggtctgaata cgccctttat gtaccggcaa
     4801 tacaagcgat gttcccagca ttgaggattt gatatgagaa ggcatatcgt ctgctccctc
     4861 atagtcatgc tcataatttc cgttgtcggg aacggtgcgg aggaaaaaac gctccatgtc
     4921 gtggcgtacg gtgggatcgc agttctcatt aagtgtcaga gaggcggagg tatgttgcag
     4981 caacagatgc agtaagccga tgttaacgcg cggcatatca gccagctgat tcagaatttc
     5041 atccgttacc agatgaaacc cacgagattt ggcgctaagc gtgagcgtct tttgatacca
     5101 catgtgctgc tccttgataa aactctctta atcagtttgc agcaagacag cgaaaggata
     5161 aaggtgtgat taaaaaaaca gcattcggga gagcgtcacg ctctcccgct ctgtcagtat
     5221 tctgaattga cgatcacctc ttcaccaaac gcacccgctt gtggcaaggg tttgtaggta
     5281 gagttggagg cgcgcagaat gcggatacca cgagcgccga catcgcgtgc ggcggtaata
     5341 tcattatcag aatcgccata aaaaattcgg atatttttat cctgcagcca ttgcgatttt
     5401 gtattttgcc ctagtttatc gcccgcaaag atcaccggat tcatgttggt ggcaggaata
     5461 tgaaaattat ccgccagcgt ttttgaaacc gtttctgttt tcgtcggact acgaccagtc
     5521 acaaagaaga tcgcgtcacc gcggcgtaca tgcatatcaa tcagctggcg agcgacctct
     5581 tttggaatgc tgaattcatc ccagccattg ttcatttttt cccagaacac aggatttttc
     5641 agataatctt cgctttctgg cgagaaggtt tttttgccgc gccagaagcc cggactggaa
     5701 aaaagtaccg tgtcatcgat atcaaacccc accgccattg gcggacgccc tgcgaggcta
     5761 ttttcaattt gtgcgaccga aacccaatga atgggtgcct gttcagcaag cctggcaacg
     5821 ttagtcccag ggttaagcgg tgaaggagat gaggccaggg caacagcgga actgtttaga
     5881 gcgaacaata agcaaacggc actgattgcc tgtgtgatct tgcgcatatt tttccctaaa
     5941 tagtcagtat gttgaaactt tttattgtga gatttgttgc aaaaaccatc tgaccataac
     6001 gccagctgcc ggggaaagga aggattatct gcggtttttg tgatgaaaag caggatgatt
     6061 atatttaacc cagtaaatac gttcctgtct ttttttgtat aaaaaatgtt tattactcga
     6121 tgtaaggata cattaatttt acgtttatta tgatgaatcc tcggcaaatg agcgaaattt
     6181 gatagaacat ttatctgctg tacgatttaa tgaacttaat gatacagcat tacaaaaaca
     6241 atcgaagttt ataaagatga tttctgattg accagccccc taccctacaa tggtgattaa
     6301 ttttattact cgcgatggag atgatgatat gaatatatca tatgtgaata gcaacaaaac
     6361 gacatcttta cctgttgagc tagatgcact aaataataaa gatatctctt atgccaaaga
     6421 tttttcttat gccaaagatt tctttttata catagaaaca cagttaaaaa ttgcaaaaga
     6481 tttttgtaga cctggagaag aagtatcaag ttctattgca agtaaagttt ttcacgcatt
     6541 tattgattta gttaacaaaa tcaggggtaa gaaagacttt atgtatattt gcacgctttg
     6601 ctgttttgcc gaggaggtta aaggtgatta ttctcattac aggacctttt tatttgatat
     6661 tggtaatcaa tataaggtta aacttacaca aagcggaaaa aaagagctct ctttaacttt
     6721 agaatttaac gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat
     6781 tc
//
LOCUS       DAZGOB010000195         6761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGOB010000195 DAZGOB010000000
VERSION     DAZGOB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.195"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1261
                     /locus_tag="ACHLIF_004693"
     CDS             <1..1261
                     /locus_tag="ACHLIF_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO9608546.1"
                     /translation="DNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPIWNWKISQYN
                     VFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLGI
                     MAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQPYLVI
                     NKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNCNGNYM
                     QNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPITVSSDE
                     MTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLAC
                     SQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEIT
                     TPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1451..1564
                     /gene="yhiY"
                     /locus_tag="ACHLIF_004694"
     CDS             1451..1564
                     /gene="yhiY"
                     /locus_tag="ACHLIF_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO9608547.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1627..2694
                     /gene="yhiI"
                     /locus_tag="ACHLIF_004695"
     CDS             1627..2694
                     /gene="yhiI"
                     /locus_tag="ACHLIF_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9608548.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSESRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2691..5426
                     /gene="rbbA"
                     /locus_tag="ACHLIF_004696"
     CDS             2691..5426
                     /gene="rbbA"
                     /locus_tag="ACHLIF_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO9608549.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDSLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5426..6550
                     /gene="yhhJ"
                     /locus_tag="ACHLIF_004697"
     CDS             5426..6550
                     /gene="yhhJ"
                     /locus_tag="ACHLIF_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9608550.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1595 a         1668 c         1872 g         1626 t
ORIGIN      
        1 tgataatacc gatattaatt ggtgcagcat tgtttattta cttaataata tgacagtaaa
       61 tgatgatgct aatgatgttg cagttactga gtcgtacaaa cccatctgga actggaaaat
      121 atcgcaatac aatgtatttg atattaaatt cgaaacaatg ataaaacccc agttcgctga
      181 cagaacctat ttctccaatt gttcacctgt tgatcccaca agtaccagac caacctattt
      241 tggcgatacc gatggctcag tcggtgcagt tttgtacgcg ctttttgcaa cgggacattt
      301 gggaatcatg gctgaaggag aaaatttcct tagccagttg cttaatatag aagatgaagt
      361 tctcaacgta ttgctaaggg agaactttaa tgagcaactt gacactaatg ttaatataat
      421 aatcagtatc ctgaacagaa gagacattgt tttagaaagt cttcagcctt atttggttat
      481 taataaagac gcagtcacgc catgtacgtt tctgggcgac cagactggcg atcgatttag
      541 taatatctgt ggcgataaat ttatcatcga tttattaaaa cgcataatga gtatcaatga
      601 aaacgttcat gtattagctg gaaatcatga aacgaattgc aatggtaact atatgcagaa
      661 cttcacgcgc atgaaaccac ttgatgagga tacctacgct gggataaaag attatcctgt
      721 atgtttctat ggccccaaat acaaaataat ggcaaatcat catgggataa cttttgatga
      781 tcagcgaaaa cgttacatta taggaccgat aacagtctct agtgatgaaa tgaccaatgc
      841 tctcgatccg gtcgaattag cggcaattat caataaaaag caccatgctg taattaatgg
      901 caaaaagttt aaaaatagcc gggctatctc ttgtcgatcc tttaatcgtt atttttctgt
      961 ttcgacagat tacagaccta aacttgaagc actattagca tgctcacaaa tgttaggtat
     1021 aaatcaagtt gttgcacata acggtaatgg tggaagagaa tgtataggtg aaacggggac
     1081 agttttaggt cttaacgccc gtgacagtaa acatgctgga agaatgttca gcatgcataa
     1141 ttgccaaatt aaccctggtg cagggcctga gataaccact ccctggaagt cttaccagca
     1201 tgaaaaaaac agaaatggac ttatgccttt gattagaagg cgcacaatgt tacagctctg
     1261 aaccatagac aacatgaata ttaatgttac tatctggttg agactgttga taaaacgtaa
     1321 aaaggaatgc tctgttcctg caactgaaca ggcgtgaaac gcgatccaac aggatggata
     1381 ccgttttgtc agccagtttt atagatcaca cttatgagta tgcgtgtttc atttacggcc
     1441 aggaatgctg atgatgacaa cattactacc cgttttcact aagccttccc cgttggcgct
     1501 caatgctctg cgcgctggtc gtatttgtcg tttccttctt atcccggatg gaagaatccg
     1561 ctgaacgtcg ttcggcggca gttgtaacac gcgtcgtttg cttacaggga ttatggcggg
     1621 ccgattatgg ataagagtaa gcgccatctg gcgtggtggg ttgtcggggc actggcggtg
     1681 gctgccgtcg tggcgtggtg gctgttgcgc ccggcgggtg tgccggaagg ctttgctgtc
     1741 agcaatgggc gcattgaagc gacggaagtg gatatcgcca gcaaaattgc cgggcgtatc
     1801 gacaccattc tggtgaaaga aggccagttt gttcgcgaag gtgaagtgct ggcgaagatg
     1861 gatactcgcg tgttgcagga acagcgactg gaagccatcg cgcaaatcaa agaggcacaa
     1921 agcgccgttg ccgccgcgca ggctttgctg gagcaacgac aaagcgaaag tcgcgccgcg
     1981 cagtcgctgg ttaatcaacg ccaggcagaa ctggactccg tagcaaaacg tcatatgcgt
     2041 tcccgttcac tggcccaacg aggggctatt tctgcgcaac agctggatga cgaccgcgcc
     2101 gccgctgaga gcgcccgagc tgcgctggaa tcggcaaaag ctcaggtttc tgcttctaaa
     2161 gccgctatag aggcggcacg taccaatatc attcaggcgc aaacccgcgt cgaagcggca
     2221 caagccactg aacggcgcat tgccgcagat atcgatgaca gcgaactgaa agccccgcgt
     2281 gacggacgcg tgcagtatcg ggttgccgag ccaggggaag tgctggcggc aggcggtcgg
     2341 gtgctgaata tggtcgatct cagcgacgtc tatatgacct tcttcctgcc aaccgaacag
     2401 gcgggcacgc tgaaactggg tggtgaagcc cggctgatcc tcgatgccgc gccagatctg
     2461 cgtattccgg cgaccatcag ttttgtcgcc agtgtcgccc agttcacgcc aaaaaccgtc
     2521 gaaaccagcg atgaacggct gaaactgatg ttccgcgtca aagcgcgtat cccaccggaa
     2581 ttactccagc agcatctgga atatgtcaaa accggtttac cgggcgtagc gtgggtgcgg
     2641 gtgaatgaag aacttccgtg gcctgacgac ctcgtggtga ggttgccgca atgacgcatc
     2701 tggaactggt tcccgtcccg cctgtcgcgc aactggcggg cgtgagccag cattatggaa
     2761 aaaccgttgc gctgaacaat attactctcg atattccggc ccgctgtatg gtcgggctga
     2821 ttggtccgga tggcgtcggg aagtcgagct tattgtcgtt gatttccggt gcccgcgtca
     2881 ttgagcaggg caatgtgatg gtgctgggcg gcgatatgcg cgacccgaag catcgccgcg
     2941 acgtctgccc gcgcatcgcc tggatgccgc aggggctggg caaaaatctc taccacacct
     3001 tgtcggtgta tgaaaacgtc gattttttcg ctcgcctgtt cggtcacgac aaagcggagc
     3061 gggaagtgcg aattaatgag ctgctgacca gcaccgggtt agcaccgttt cgcgatcgtc
     3121 cggcagggaa actctccggc gggatgaagc aaaaactggg gctgtgctgt gcgttaatcc
     3181 acgacccgga actgttgatt cttgatgagc caacaacggg ggttgactcg ctctcccgcg
     3241 cccagttctg ggatctgatc gacagtattc gccagcggca gagcaatatg agcgtgctgg
     3301 ttgccaccgc ctatatggaa gaggccgaac gcttcgactg gttggtggcg atgaatgccg
     3361 gagaagtgct ggcaaccggc agcgccgaag agttgcggca gcaaacgcaa agcgccacgc
     3421 tggaagaagc atttataaat ctgttaccgc aagcacaacg ccaggcgcat caggcggtag
     3481 tgatcccacc gtatcaacct gaaaacgcag agattgccat cgaagcgcgc gatctgacca
     3541 tgcgttttgg ttccttcgtt gccgttgatc acgtcaattt ccgcattcca cgcggggaga
     3601 tttttggatt ccttggttcg aacggctgcg gtaaatccac caccatgaaa atgcttaccg
     3661 gactgctgcc cgccagcgaa ggtgaggcgt ggctgttcgg gcaaccggtt gatccaaaag
     3721 atatcgatac ccgccgtcgg gtaggctata tgtcgcaggc gttttcgctc tataacgaac
     3781 tcaccgtgcg gcaaaacctt gagttacatg cccgtttgtt tcacatcccg gaagcggaaa
     3841 ttcccgcgcg agtggctgaa atgagcgagc gttttaagct caacgacgtt gaagatgttc
     3901 tgccggagtc attgccgctc ggcattcgcc agcggctttc gctggcggtg gcggtgattc
     3961 atcgcccgga gatgttaatc ctcgatgaac ctacttctgg cgtcgatccg gtggcgaggg
     4021 atatgttctg gcagttgatg gtcgatctct cgcgccagga caaagtgact atcttcatct
     4081 ccacccactt tatgaacgaa gcggaacgtt gcgaccgcat ctcactgatg cacgccggaa
     4141 aagtgcttgc cagcggtaca ccgcaggaac tggttgagaa acgcggagcc gccagtctgg
     4201 aagaggcatt tatcgcctat ttgcaggaag cggcagggca gagcaacgaa gccgaagcgc
     4261 cgcccgtggt acacgacatc acccacgcgc cgcgtcaggg atttagcctg cgccgtctgt
     4321 ttagctacag ccgccgcgaa gcactggaac tgcgccgcga tccggtccgt tcgacgctgg
     4381 cgctgatggg aacggtgatc ctgatgctga taatgggtta cggcatcagt atggatgtgg
     4441 aaaacctgcg ctttgcggtg ctcgaccgcg accagaccgt cagtagccag gcgtggacgc
     4501 tcaatctctc cggttcccgt tactttatcg aacagccgcc gctcaccagt tatgacgagc
     4561 ttgatcgtcg gatgcgtgcg ggcgatatca cggtggcaat tgagatccct cccaatttcg
     4621 ggcgcgatat cgcgcgtggt acgcctgtgg aactcggcgt ctggattgac ggagcgatgc
     4681 ccagtcgtgc cgaaacggta aaaggttacg tgcaggcaat gcaccagagc tggttacagg
     4741 atgtggcgag ccgacaatcc acacccgcca gccaaagcgg gctgatgaac attgagacgc
     4801 gttatcgcta taacccggac gtaaaaagcc tgccagcgat tgttccggcg gtgatcccgc
     4861 ttctgctgat gatgatcccg tcaatgctaa gcgcccttag cgtggtgcgg gaaaaagagc
     4921 ttgggtcgat tatcaacctt tacgtgaccc ccaccacgcg tagtgaattt ttgcttggta
     4981 aacagttgcc atacatcgcg ctggggatgc tgaacttttt cctgctctgc ggcctgtcgg
     5041 tgtttgtgtt tggcgtaccg cataaaggca gtttcctgac gctcaccctg gcggcgctgc
     5101 tgtatatcat cattgccacc ggaatggggc tgctgatctc cacctttatg aaaagccaga
     5161 ttgccgccat tttcggaacg gcgattatca cgttgatccc ggcgacacag ttttccggga
     5221 tgatcgatcc ggtagcttcg ctggaagggc ctggacgttg gatcggcgag gtttacccga
     5281 ccagtcattt tctgactatc gcccgcggga cgttctcgaa agcgctggat ctgactgatt
     5341 tgtggcaact ttttatcccg ttgctgatag ccatcccgct ggtgatgggc ttaagtatcc
     5401 tgctgctgaa aaaacaggag ggatgatgcg ccatttacgc aatattttta atctgggtat
     5461 caaagagttg cgcagtctgc tcggtgataa agcgatgctg acgctgattg tcttctcgtt
     5521 tacggtgtcg gtgtattcgt cagcgaccgt tacgccagga tcgttgaacc tcgcgccgat
     5581 cgccattgcc gatatggatc aatcgcagtt atcgaaccgg atcgttaaca gcttctatcg
     5641 tccgtggttt ttgccaccgg agatgatcac cgccgatgag atggatgccg gactggacgc
     5701 cggacgctat accttcgcga taaatattcc gcctaatttt cagcgtgatg tcctcgccgg
     5761 acgccagcca gatattcagg tgaacgtcga tgccacgcgc atgagccagg catttaccgg
     5821 caatgggtat atccagaata ttatcaacgg tgaagtgaac agctttgtcg cgcgctaccg
     5881 tgataacagc gaaccgttgg tatcgctgga aacccggatg cgctttaacc cgaaccttga
     5941 tcccgcgtgg tttggcgggg tgatggcaat catcaacaac attaccatgc tggcgattgt
     6001 attgaccgga tcggcgctga tccgcgagcg tgaacacggc acggtggaac acttgttggt
     6061 gatgccgata acgccgtttg agatcatgat ggcaaaggtc tggtcgatgg ggctggtggt
     6121 gctggtggtg tcgggattat cactggtgct gatggtgaaa ggcgtgctgg gtgtaccgat
     6181 tgaaggctcg atcccgctgt ttatgctggg cgtggcgctc agtctgtttg ccaccacgtc
     6241 aatcggcatt tttatgggga cgatagcgcg ttcaatgccg caactggggc tgctggtgat
     6301 tctggtgctg ctgccgctgc aaatgctttc cggtggatcc acgccgcgcg aaagtatgcc
     6361 gcagatggtg caggacatta tgctgaccat gccgacgaca cactttgtta gcctcgcgca
     6421 ggccatcctc taccggggtg ccggattcga aatcgtctgg ccgcagttcc tgacgctgat
     6481 ggcaattggc ggcgcgtttt tcaccattgc actgctgcga ttcaggaaga cgattgggac
     6541 aatggcgtaa atacaattgc ccgatgcgta agcttatagg gactacgcat cggtgcaatt
     6601 tattgaattt tagttgtttt taggccggat aaggcgttta tgaactgacc cccaaaacct
     6661 ggacagtttc agttaaccgg ctttttgata ctcagtcctg tattcagggc aagattacga
     6721 aagcccgctc cccgcaagga ctgacgccag gtagtttctg t
//
LOCUS       DAZGOB010000196         6629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGOB010000196 DAZGOB010000000
VERSION     DAZGOB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.196"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..458)
                     /locus_tag="ACHLIF_004698"
     CDS             complement(<1..458)
                     /locus_tag="ACHLIF_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HFO9608551.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGL"
     gene            876..1565
                     /gene="paoA"
                     /locus_tag="ACHLIF_004699"
     CDS             876..1565
                     /gene="paoA"
                     /locus_tag="ACHLIF_004699"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO9608552.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTELLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            1562..2518
                     /gene="paoB"
                     /locus_tag="ACHLIF_004700"
     CDS             1562..2518
                     /gene="paoB"
                     /locus_tag="ACHLIF_004700"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO9608553.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIHPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            2515..4713
                     /gene="paoC"
                     /locus_tag="ACHLIF_004701"
     CDS             2515..4713
                     /gene="paoC"
                     /locus_tag="ACHLIF_004701"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO9608554.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            4723..5679
                     /gene="paoD"
                     /locus_tag="ACHLIF_004702"
     CDS             4723..5679
                     /gene="paoD"
                     /locus_tag="ACHLIF_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO9608555.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            5730..6068
                     /locus_tag="ACHLIF_004703"
                     /pseudo
     CDS             5730..6068
                     /locus_tag="ACHLIF_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            6356..>6629
                     /locus_tag="ACHLIF_004704"
     CDS             6356..>6629
                     /locus_tag="ACHLIF_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HFO9608556.1"
                     /translation="MRYKPNQYLICDSYGNYYLRITLPVYMQPFFEGKRTFVRSLHTS
                     NLRVARRKRDQIADEYHCLRESVAPVNSTIENTLELLRSKAKYAKTA"
BASE COUNT         1570 a         1834 c         1817 g         1408 t
ORIGIN      
        1 agtcccatga gagggaatga aatcatatga acaaaaagtt gggctaaagc acctgccagt
       61 aagccctgcc agagtttaat ttttggaaat acttcagcga ccacacaata accgacagca
      121 aacactatcg aaaagataat gtgcgtaaca ccaacccagt taaagacatg cccggcaaag
      181 gtataaacag ccgcattggg atctgtcagc cccaaccagt ctcgaagaaa aatatacggt
      241 ggattgagaa aattacgcga gcaatcaatt tggcctgcag ccctgattaa tgattccggg
      301 ccacacgctg cattaaacat atccaccggg ctacgtggcg gcaatggaac ttcagccccc
      361 cacttcacga atgcggaaac aacgccagca atcagcccaa tgaatgcagc aagaccataa
      421 cgtctgcggt tcggtggagt ttgttcaaat atattcatat ctaccctgct tgtaccatta
      481 tgttatacac ctcttcagga gtattcataa aacaaggcaa atgtaaagaa ctgtattgtt
      541 ttgtataaca agatagtttc ctaatcgcca atgaatataa gctccatcat ttctccctat
      601 ttttatatta gaagtgacag agatttgcag ggtgatgcag agctgaaaat cacaggtttc
      661 cttattggtt tttgcatcgt acaactaaag caataaacca gctcccatca tcatcaacat
      721 cccggcgaac atgaatttat tagccgggct catagctgca ttcgcgtttt aagaataatc
      781 ctcctgctgt cgccgactat gcttaacgtt taaaaaagca tcagcactct cgcaacgcac
      841 tcttattttc ccctttagaa taccggaggc ctggtatgag caaccaaggc gaataccccg
      901 aagataatcg ggttgggaag cacgagccgc acgatttaag tttgacccgt cgcgatctga
      961 ttaaagtgag cgccgcaaca gcggcgaccg ccgtggttta tcctcattct acgctggcgg
     1021 caagcgttcc ggcagctaca cccgcgccag agataatgcc cctgacactg aaggtgaacg
     1081 gcaaaaccga gctgcttgag gtggataccc gaaccacgct actggacgct ttgcgtgaaa
     1141 atctgcattt gatcggtacc aagaaaggtt gcgatcacgg acagtgcgga gcctgtaccg
     1201 tgctggtcaa tggtcgcagg cttaatgcct gcctgacgct tgcagtcatg catcaggggg
     1261 ccgagatcac caccattgaa ggcctgggtt cgccagataa tcttcacccc atgcaggcgg
     1321 cctttatcaa gcatgatggc ttccagtgcg gctactgcac ctccgggcaa atttgctcat
     1381 cagtagcggt gctaaaagag attcaggacg gcattcccag tcacgtcacg gtcgatttgg
     1441 tttccgctcc agaaacaact gccgatgaga tccgtgaacg tatgagcggc aacatctgtc
     1501 gctgtggtgc atacgctaac atccttgccg ccattgaaga tgctgcgggg gagataaaat
     1561 catgaaggcg tttacctatg aacgagtgaa caccccagcc gaggcggcac ttagcgctca
     1621 gcgcgtaccc ggcgcaaaat ttatcgcggg cgggaccaat ctgctggacc tgatgaagct
     1681 ggaaattgaa acgcccaccc accttatcga tgtgaacggc ctcgggctcg ataagattga
     1741 agtgaccgac gcgggtgggc tgcgcatcgg cgcactggta cggaacaccg acctggcggc
     1801 tcacgagcgc gtgcgtcgtg attacgcggt actctcccgc gccctgctcg ctggcgcatc
     1861 tggtcagtta cgtaatcagg caaccaccgc aggtaatctg ctccagcgca cgcgctgccc
     1921 ctatttttac gacaccaatc agccctgcaa taagcgcctg cccgggagcg gctgcgcggc
     1981 gcttgaaggc tttagccgtc agcacgcggt ggtaggcgta agcgaagcct gcattgccac
     2041 ccatccgagc gatatggcgg tcgcaatgcg gttgctggat gcggtggtgg aaaccatcac
     2101 gccggaggga aagactcgca gtatcacact ggctgatttt tatcaccctc cgggaaaaac
     2161 gccgcacatt gaaaccgccc tgcttcccgg tgagcttatc gttgcggtga cgttacctcc
     2221 gccgctcggc ggaaaacata tctaccgtaa ggtgcgcgat cgcgcctcct acgcctttgc
     2281 cctggtatcg gtcgcggcga ttattcaccc tgacggcagc gggcgcgtcg cgctgggcgg
     2341 agtagcacat aagccctggc gcattgaggc tgcggatgct cagctatccc agggggcgca
     2401 ggccgtatat gacacgctgt tcgccagcgc ccatcccacc gctgaaaaca cctttaaact
     2461 cctgttggcg aagcgaacgc ttgcctccgt actggctgaa gcgagggcac aggcatgaaa
     2521 tttgataaac ccgcagggga aaacccgatc gatcagctga aggttgtcgg tcgtccccat
     2581 gaccgcatcg acggaccgct gaaaactacc ggcacggcac gctacgccta cgaatggcat
     2641 gaagaagccc ccaacgccgc ctatggctat atcgtcggtt ccgccattgc caaaggacgc
     2701 ctcaccgccc ttgatacgga cgccgcgcaa aaagcgccgg gcgtactggc tgtcattacc
     2761 gccagtaacg ccggggcact cggcaaaggc gacaaaaaca ccgccagact gttaggcggc
     2821 cccactattg agcactatca tcaggccatt gcgctggtag tggccgagac cttcgaacag
     2881 gcgcgagcgg cggcctcgct ggtgcaggca cactatcgcc gtaataaagg agcttactcc
     2941 ctggcggacg aaaaacaggc cgtcaatcag ccgccggaag acacgcccga caaaaacgtc
     3001 ggtgactttg acggggcttt cacctccgct gcggtgaaga ttgatgctac ctacacgacc
     3061 ccggaccaga gccatatggc gatggagccg catgcctcga tggccgtctg ggatggaaat
     3121 aagcttaccc tctggacctc aaatcagatg attgactggt gccgcaccga tctggcaaaa
     3181 acgctgaaag ttcccgtgga gaatgtgcgt attatctccc cgtatatcgg cggagggttt
     3241 ggcggcaagc tgttcctgag aagcgatgcg ctgctggcgg ccctcgccgc ccgagcggtg
     3301 aaacgtccgg ttaaagtgat gctcccccgc ccctctattc ccaataacac cacgcaccgc
     3361 cccgccaccc ttcagcactt gcgtatcggt gccgaccaga gcgggaaaat caccgctatc
     3421 tcacatgaaa gctggtctgg aaacctgccc ggcggcacgc cggaaacggc ggtacagcaa
     3481 agcgaattac tctacgccgg ggcgaatcgt cataccggcc tgcggctcgc cacgcttgat
     3541 ttgccggaag ggaacgccat gcgtgcgccc ggcgaagccc ccggtctgat ggcgctcgaa
     3601 atcgcgatcg acgaactggc ggaaaaagcg ggcatcgatc ccgtcgagtt tcgcatcctg
     3661 aatgacactc aggttgaccc cgccgacccg acgcgctgct tctctcgccg tcagcttatc
     3721 gagtgcttgc gcaccggagc ggataaattt ggctggaagc agcgcaacgc cacacccgga
     3781 caggtgcgcg acggggagtg gctagtcggc cacggtgttg cggcgggctt tcgcaataat
     3841 ctgctggaaa aatcgggtgc tcgggttcac ctcgaacaaa acggcaccgt taccgtagaa
     3901 acggacatga ccgacattgg caccggcagc tacaccattc tggcccagac ggcagcggaa
     3961 atgcttggcg taccgctgga gcaggttgcg gttcacctcg gcgattccag tttcccggtt
     4021 tctgcgggtt ctggtggaca atggggcgcg aatacctcca cctccggcgt ttacgccgcc
     4081 tgtatgaagc ttcgcgaaat gattgcctcg gcagtcgggt ttgatcctga gcagtcgcag
     4141 tttgccgacg gcaagattac caacggtacc cgaagcgcca cgctacatga agccaccgca
     4201 ggcggcagac tgacagcgga agagagcatt gaattcggaa cactgagcaa agagtaccag
     4261 cagtcgacct ttgccgggca ttttgtggag gtcggcgtgc atagcgcgac gggagaagtt
     4321 cgggtccggc gtatgctcgc tgtgtgtgct gcaggacgca tcctgaatcc gaaaactgcg
     4381 cgcagccagg tcattggcgc aatgactatg ggcatgggcg cggcactgat ggaggagctg
     4441 gcggtggatg accgtttggg ctacttcgtt aatcacgata tggcggggta tgaggtgccg
     4501 gtccatgcgg atatcccaaa acaggaggtg attttcctgg atgataccga ccccatatcc
     4561 tccccgatga aggccaaagg tgtcggtgag ctgggcctgt gcggcgtgag cgcggctatc
     4621 gccaacgcgg tgtataacgc caccggtatt cgggtacggg attatcccat cactctggat
     4681 aagctgctcg ataagctgcc ggatgtggtt taaggagaaa caatgtcata cccgcttttt
     4741 gataaagacg aacactggca taagccagag caggcgtttc tcaccgatga ccaccggacc
     4801 attctgcgct tcgccgtaga ggcgctaatg tccggtaaag gagcggtgct ggtgacgctg
     4861 gtggagatac acggcggcgc ggcgcgcccg ctcggggcgc agatggtggt tcgcgaagat
     4921 ggtcgttact gcggttttgt ctctggcggc tgcgtggagg ccgctgccgc ttttgaagcg
     4981 ctggagatga tgggctcagg ccgcgatcgc gaaattcgct atggcgaagg ttcgccgtgg
     5041 tttgacatcg ttctgccctg cggcggtggg atcacgctga cgctccataa actacgctcg
     5101 gcacagcctc tgctcgccgt gctgaaccgt ctggaacaga gaaaaccggc ggggctgcgc
     5161 tacgatccgc aagcacaatc gttggtgtgc ctgcccacgc aaacccgaac gggctggaat
     5221 ctcaatggct ttgaggtggg gttcaggcca tgcgtcaggc tgatgattta cggacgttct
     5281 cttgaggcgc aggcaaccgc gagtcttgca gcagccacag gctatgacag ccatatcttc
     5341 gatctttttc cggcctcagc cagcgctcag atcgataccg acacggcggt cattttgctg
     5401 tgccatgatc tcaaccggga gctgccagca ttgcaggccg cgcgagaagc aaaacccttt
     5461 tatctcggcg cattgggcag ccatcgaacc cacacgttac gtctgcaaaa gctccacgag
     5521 ctgggatggt ccagggagga gacagcgcaa atccgggccc ccgtcgggat attccccaaa
     5581 gcccgggatg cgcatactct ggcactctcc gtgctggcag aaatcgcctc tgtacgtctc
     5641 catcatgagg aggattcatg cctgcccccg tcgtcctgat ccttgcggcc gggcgtggag
     5701 agcgctttct cgcctccggg ggaaataccc ataagtgtat cggctggcgt cagtccccgg
     5761 aggttgcgcc ttatcgctgg ccatttgaag aaaacgggag aactttcgac cttgcgattg
     5821 aaccgcagat tacgactaat gatctgcgtc tgatggtgag gctggctctt gccggcggag
     5881 gaataacaat tgccactcag gaaactttca ggccatatat tgaaagcggt aagcttgtat
     5941 cgctgcttga tgactttctt ccacaatttc cgggcttcta tctgtatttc ccacagcgtc
     6001 gcaatattgc accaaagctc cgcgccctga ttgactacgt caaagaatgg cggcagcaat
     6061 tggtttaaat agctgcacct gcactgcctg atgtcagaac agtattttga tgaattgcca
     6121 aggttacaat ggcacaaatg cggcacagga ggaaacgtgg tgtatttaaa tatggggtaa
     6181 ctctttgatt ttaatggtgc cgataatagg agtaaaacac tcgattaatc tcgtgttaat
     6241 gccatcacct acgaccatta ctaattgatt gaatcttata gaaaaatcat cattttctat
     6301 gcgtcacaat caattttcac attacaaacg ctccgacact ttagccggag attttatgag
     6361 atacaagcct aaccaatact taatctgtga ttcttacggt aactactatt tgaggatcac
     6421 gttgcctgtg tatatgcagc ccttttttga aggaaaaagg acgtttgtca ggagtctaca
     6481 tacaagtaat cttcgtgttg cacgtagaaa gcgtgatcag attgcagatg aataccattg
     6541 tctacgggag agtgtcgccc ctgtaaacag cacaatagag aacacgctgg agctgttacg
     6601 cagtaaggct aaatacgcca aaacagcta
//
LOCUS       DAZGOB010000197         6417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGOB010000197 DAZGOB010000000
VERSION     DAZGOB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.197"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            88..297
                     /locus_tag="ACHLIF_004705"
     CDS             88..297
                     /locus_tag="ACHLIF_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608557.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(298..936)
                     /locus_tag="ACHLIF_004706"
     CDS             complement(298..936)
                     /locus_tag="ACHLIF_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608558.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(948..1100)
                     /locus_tag="ACHLIF_004707"
     CDS             complement(948..1100)
                     /locus_tag="ACHLIF_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9608559.1"
                     /translation="MDTLNLGNNESLVCGVFSNLDGTFTAMTYTRSKTFKTEAGARRW
                     LARNTD"
     gene            complement(1267..1674)
                     /locus_tag="ACHLIF_004708"
     CDS             complement(1267..1674)
                     /locus_tag="ACHLIF_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9608560.1"
                     /translation="MDTKNLTIGERIRYRRKNLKYTQRSLAKVLKISHVSVSQWERDD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPSPPIEEPVALSPKEHELLELFNALPES
                     EQDAQLTEMRARVKNFNKLFEELLKARQRTNKR"
     gene            1758..1988
                     /gene="dicC"
                     /locus_tag="ACHLIF_004709"
     CDS             1758..1988
                     /gene="dicC"
                     /locus_tag="ACHLIF_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HFO9608561.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGELVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            1972..2493
                     /locus_tag="ACHLIF_004710"
     CDS             1972..2493
                     /locus_tag="ACHLIF_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9608562.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRITFNDGEVDQRALGVNRVKIFERWKAIDTRDKREKFTALIPAIMEAIRISDFRLY
                     REITDGKSITYMIAGLNKEYGDVVESGLLFADPAVVERETDELIEKAIAFKRAYRHQY
                     QQKAGWNYEPSFC"
     gene            2474..3439
                     /locus_tag="ACHLIF_004711"
     CDS             2474..3439
                     /locus_tag="ACHLIF_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608563.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTSSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKALGLLRSEKLNKSKRDMTNFYTINY
                     DSALLDDGKVNESIGSKCATPSGQNDTMEEVKMKRSIGSKRPNVIRSKWPDDPTENTT
                     ENTTENKKLSCPDASQPDELTAEQAFLTRHPDAVVFSVKKRQWGTREDLTCAQWIWGR
                     VVNLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFSRAQRDPFWI
                     KNIKSPEKLREKWDELVIRLWRGPAQRCVNHISEPDTEIPPGFRG"
     gene            3446..4186
                     /locus_tag="ACHLIF_004712"
     CDS             3446..4186
                     /locus_tag="ACHLIF_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9608564.1"
                     /translation="MKNITSGGVLARVSRFVPQDAIPPYRTVTEWREWQLAEGRKRSE
                     EVNRLNHQTRVEKIINRSGIQPLHRKCTFGNYRVQNDGQRHALSQAKSIAAELEGGCT
                     NFVFSGRPGTGKNHLAAAIGNHLLAKGRSVIVITVADVMLALHGSYDNKNSGEKFLQG
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASLLSVGMLTNLNHAAMNTLLGERV
                     MDRMSMNGGRWVTFNWESWRPNVSQHRN"
     gene            4216..4977
                     /locus_tag="ACHLIF_004713"
     CDS             4216..4977
                     /locus_tag="ACHLIF_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9608565.1"
                     /translation="METVLHALKTMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAGEGESGVTEEQPAQSEVPDVLTGEVEQKVTADMMIEFICQDGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCIPGADLPAEPEAASVAETDGKA
                     FPQPACVALPVREAETQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKCRDILRDITATREQQR"
     gene            4974..5153
                     /locus_tag="ACHLIF_004714"
                     /pseudo
     CDS             4974..5153
                     /locus_tag="ACHLIF_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            5574..6125
                     /locus_tag="ACHLIF_004715"
     CDS             5574..6125
                     /locus_tag="ACHLIF_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO9608566.1"
                     /translation="MTTQVTVKNLSPVTYNQIPVITTELLARLYGTEAIRIQQNHHEN
                     KSRFIEGKHFFKAVGDELKNLRLVLNESQNEVKISPKTRTLILWTERGAARHAKMLET
                     DQAWEVFEKLEDSYFNQYEKERAMGKMNIDFHLAIRDDKTAFIRSYAPGQLVTVEEAL
                     ALLKSRGWLVMPRDELAGKLMEL"
BASE COUNT         1722 a         1390 c         1714 g         1591 t
ORIGIN      
        1 ccggattctt atccgtgtcc ggcgcacgac cacacgtgac agcgtgttgg tctccatttt
       61 taacccagaa cctcaatgga ggataaaatg ccaaacaaaa aaagaaatcc gcttattgaa
      121 aaacagattg aatgcttggt aaatcaactc aggcaatcag ggttattaaa aactcattca
      181 gagttgaggc tcacagaatc agcattcgac gataaattaa ataatgtcct ttataatggc
      241 attattgatt ttaatcgttc tgttggtcgc cgcggccctg ctggtgtttc cttataatta
      301 ccagtcaatc cagagtggac cgtgttcagc gtaaatataa ctgtacacat ccagattata
      361 tttgtggtct gttaagaaca ggccgcaaat acatgccgaa gcttccagtg cagcggctct
      421 gttactgaat aaccatgtag caacattcca gcgtttttct gcatcccagt ctttctcaag
      481 gcctgatacc atgaagaaac cgttagtgtt gccatcaaat aattctgttt ccaaattttt
      541 aagcaatgcc tgatggactc ttgccaggta ttccgccgga atttcgccac gaattctgat
      601 gagattgtca taaacaaaca tgttccccgc atatggcgat ttttctttct tgttttttaa
      661 accagcatca tgagcaaact gatcaatttc ttcttccgtt ggtttcgtat tgatgttttg
      721 cgctgtcgtt tctgcaattt tatttgccac actctctgag tcgtgtttat ttatagacgc
      781 acagaaatac aatccggtaa acgcatcgcg cacattacga gccatattat cagtgtcttt
      841 tttcgttacc gattccaatt caagttcgtt cagacgatga cgaagtgtgt gtgctgcaat
      901 ctcctggatt gaaggaggta aatctttaaa ttccatcgtc aacctcatca gtcagtgttt
      961 ctggctaacc agcgacgcgc gccagcttca gttttaaacg ttttgcttct ggtatacgtc
     1021 atcgcggtaa acgtgccgtc caggttggag aatactccgc ataccagaga ttcgttgttg
     1081 ccaagattga gcgtatccat gttgacctca tttcccctta acgccggggt agcggaactg
     1141 tttgctgaga acaccgtgcg gtgtcttgat gcaagcaaga ttagccatga ctaacaattt
     1201 ggtcaagtat ttttgtttgc catgactaac attttgggca accaaaaaga taacgcattg
     1261 attgcgttat cttttgtttg ttcgttgacg ggcttttaat aactcttcaa agagtttgtt
     1321 gaaatttttt actcgggcgc gcatctcggt gagctgagca tcctgttctg attcaggcaa
     1381 tgcattaaaa agctcaagga gctcatgttc tttgggggat agagcaactg gctcctcaat
     1441 aggtggtgat ggctgcttgt cttcatcgcc aaatagaatc catgttggcg agcactgcag
     1501 tactttgctg agggcaaaaa gattctttcc tgtaggttcg ctatcatccc gctcccattg
     1561 tgaaaccgat acgtgagaaa ttttcaggac tttagcaaga gacctttggg tgtatttgag
     1621 gttttttcgg cgatatctaa tgcgttcgcc aatggttaaa ttttttgtat ccatagttag
     1681 ctaatgctaa atcttattga ctatgttttt gttaacatct attttgttag tcatggctaa
     1741 caatcaaggt gctttaaatg cttaaaactg acgcactttt gtatttcggt tcaaaaacaa
     1801 aacttgcaca agctgctggt attcgtttgg cttcgcttta tagctggaaa ggggagctag
     1861 tacctgaagg tcgcgcgatg cgcctgcaag aggcatccgg cggggaactt cagtacgacc
     1921 ccaaagttta tgacgaatat cgtaaggcaa agcgggcggg gcggttgaac aatgaaaatc
     1981 acccctgaac aggtttgtga ggctctggat gcctgggtgt gccgaccagg aatgacacag
     2041 gaacaggcga cgatattaat cacggaagca ttctgggctc tgaaagaacg cccgaacatc
     2101 gatgttcaac gcatcacgtt taatgatggc gaggttgatc aacgggcact gggcgttaac
     2161 cgggtgaaga tattcgaacg ctggaaggct atcgacacca gggataagcg tgaaaaattc
     2221 acggcgctga ttccggcaat tatggaggct atccggatta gtgatttcag gttgtaccgc
     2281 gaaattactg acggaaaaag cattacgtac atgattgccg ggttaaacaa agaatatggc
     2341 gatgtggtgg agtccgggct gctttttgca gatccagctg ttgtggaacg tgagacagac
     2401 gagcttatag aaaaagcaat cgctttcaag cgtgcctatc gtcaccagta ccaacaaaaa
     2461 gccggatgga attatgagcc ttctttttgc tgaacgcccg ctggttataa acacacagct
     2521 ggcgatgaag attggtttaa acgaagccat tgtgttgcag caactgcatt actggttgag
     2581 ggataccagt tccggcatgg aatgtgatgg cgttcgctgg atttacaaca caacggaaca
     2641 atggctggaa cagtttccgt tctggtcaga gtcaacgtta aagcgtgcgt ttgcaagtct
     2701 gaaagcgctg gggcttttgc gtagcgaaaa gctcaataaa tcgaagcgtg atatgactaa
     2761 tttttacaca attaactacg atagcgcgct tttagatgat ggcaaagtga acgaatccat
     2821 cgggtcaaaa tgcgccactc catcaggtca aaatgacacg atggaagagg tcaaaatgaa
     2881 acgttccatt ggttcaaaac ggcccaatgt catcaggtca aaatggcccg atgatcctac
     2941 agagaataca acagagaata caacagagaa taaaaaactc tcttgtccgg acgcttcgca
     3001 accggacgag ttgacggctg aacaggcgtt tttaacccgc catcctgatg cagttgtgtt
     3061 cagcgtgaaa aaacgccagt ggggaacccg ggaggatttg acatgtgcgc agtggatctg
     3121 ggggcgggtt gtaaacctgt acgaacaggc tgccagtgac gatggtgaga tcacgcgacc
     3181 gaaagagccc aactggacgg cgtgggccaa tgacgtgcgc acaatgcgga tgctggatgg
     3241 aagaagtcac agacaaattt gcgaaatgtt cagccgggca caacgggatc cgttctggat
     3301 aaaaaacatc aagagcccgg aaaaactccg tgagaaatgg gatgaactgg ttattcgact
     3361 gtggcgtggt cccgcgcagc gttgcgtgaa tcatatttct gaaccggata ccgaaattcc
     3421 gccgggtttc agggggtagc gcatcatgaa aaacattacg tcaggtggtg ttctggcaag
     3481 agtcagcaga tttgtgccgc aggatgcaat ccctccgtac cgtacggtga cggagtggcg
     3541 ggaatggcag cttgctgaag ggcgtaagcg aagcgaggag gttaatcgtc tgaatcatca
     3601 gacgcgggtt gaaaaaatca ttaaccgctc cggtatccag ccgcttcacc ggaagtgtac
     3661 gttcggtaac taccgggtgc agaacgatgg tcagcgccat gccctgagtc aggcgaaatc
     3721 cattgcggca gagctggaag gcggctgtac gaattttgtt ttcagtggca ggcctggcac
     3781 aggaaaaaac cacctggcgg cggctattgg caaccacctt ctggcgaaag gtcgcagtgt
     3841 gattgtgata acagtggcgg atgtgatgct ggcgttacat ggcagctacg acaacaaaaa
     3901 ctcgggcgaa aaatttttgc agggattgtg tggcgttgac ctgctggtac tggatgaaat
     3961 tggcatgcaa cgggatacgc gtaatgagca ggtcacgctg aatcagattg ttgaccgcag
     4021 aacggcatcg ttactcagtg tggggatgct gacaaatctt aaccatgcag cgatgaatac
     4081 acttctcggc gagcgggtga tggaccgcat gtccatgaac ggtggtcgct gggtgacgtt
     4141 taactgggag agctggcgtc cgaacgtcag ccagcacagg aactgagaag taatttttat
     4201 ccggaggaag ttttaatgga aaccgtattg catgcactga aaacgatggg taaagccaat
     4261 tctgttgaac tggcggcgcg gcttgatatc agccgtgaag aagttctcaa cgaactgtgg
     4321 gaactcaaaa aaaatggcgt tgttgataaa acgggtcaca cctggtttct ggctggtgaa
     4381 ggtgaatccg gggtaaccga agaacagcca gcacagtctg aagtaccgga tgtgctgacc
     4441 ggggaggtcg aacaaaaagt taccgcggac atgatgattg agtttatctg tcaggatggg
     4501 gctaaaacgt gtgaggaact ggcggataag ttcggtgtta gcattcgcaa ggttgcttcc
     4561 acgttggcgg tcgtaacagc aacggggcgc ctggcacgcg taaatcagaa cggtaaattt
     4621 cgttactgca taccgggcgc tgatttaccg gcagagccgg aagctgcatc cgtagcggaa
     4681 accgatggta aagcctttcc tcagccagca tgtgttgcgt taccagtccg ggaagcggaa
     4741 acacaggaag aaataaaaac ggaaagtgtg gcggtcacag tgcagtcaca gccgtcgttc
     4801 accagaaagc atccggatgg gctgatttta ccatcgctgc atgtggctaa ccgcgagctg
     4861 cgccgggcaa aaggtcaggt tcagaagtgg gagcgagtct gcgccgcgct gcgggagctg
     4921 aacaagtgcc gggatattct ccgggatatt accgccacca gagaacagca gcggtgagtg
     4981 ggtggaagac gtggtgccgg gctgaaattc tgatactccg gcagtgcgcg ggaacgatga
     5041 ctgtcgaaag tatcggcagt ctgattggtc gtagcgaggc agcggtcaga gcgaaagccc
     5101 gggataaggg aataagcctg atgctgcagg gtgattttcg cctgtcagcc aaattaccgt
     5161 ataaagcgat ggactgcctg aagcgaaacg gactgattgg cgctgaggat agtttacgat
     5221 gacctggcct gaggcattcg taacggcagg agttgcaatg gcggtagcgc tggtggttta
     5281 ttcgatttgc cgctggggtt gacgtaatta ctaatccggg gctatattca ccgcacggtc
     5341 gagttgaccg tcgggattga gaccccggat attcaaccgc gacagacaca cgccgcgagc
     5401 gtgtttttta ttgtcgtatg cgcacgcaca tctgaattat ggtgaggcgt atggggcagc
     5461 cgaaaggctg gccggttggt tgatccggta gtctcaaccc tgtacgtctc gccacctgat
     5521 gattgagacc tgaaggtggt gatagttaaa ttcatcaacc agagggcgtt atcatgacga
     5581 ctcaagttac tgtcaaaaac ctttcccctg tcacttataa ccagattcct gtaatcacaa
     5641 ctgagttact cgcccgcctt tacggaacag aagcaatccg tatacagcag aatcaccatg
     5701 aaaataaatc tcgatttatt gagggaaaac acttctttaa agctgttggt gatgagctca
     5761 aaaatttgcg actagtttta aacgagtcgc aaaatgaggt gaaaatttct cccaaaaccc
     5821 gcactctcat cctctggaca gaacgcggag cagcccgtca tgcaaaaatg ctcgaaaccg
     5881 atcaggcgtg ggaagtattc gaaaaactcg aagacagtta cttcaaccag tacgagaaag
     5941 agcgggctat gggaaaaatg aatatcgatt ttcacctggc gatccgtgac gataaaaccg
     6001 ccttcatccg aagttatgcg cctggccagc tggtgacggt tgaagaagcc ctggctttgc
     6061 ttaaatcgcg cggatggctg gtaatgccac gagatgaact ggccggaaaa ttaatggagc
     6121 tttaaccgat tgagttaacc ttgatatggg aattcccata tcgggagaac attaccatcg
     6181 gggataaaat gatttgcggg gaaagaagag ttaagtagaa ttgctgcggg tgcttgaggc
     6241 tgtctgcctc gggcatgccg ccgtaaggca gacagagaaa agccccagtt aacattacgc
     6301 gtcttgcagg acgcttaaca ttaatctgag gccaatttca tgctttgcac atgtaggtta
     6361 gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa ggcgatg
//
LOCUS       DAZGOB010000198         6389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGOB010000198 DAZGOB010000000
VERSION     DAZGOB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.198"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1000)
                     /locus_tag="ACHLIF_004716"
     CDS             complement(<1..1000)
                     /locus_tag="ACHLIF_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9608567.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLNCKEMNTVTGALSIIENMGSEKYLYFTVNNKEF"
     gene            complement(1083..1571)
                     /locus_tag="ACHLIF_004717"
     CDS             complement(1083..1571)
                     /locus_tag="ACHLIF_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HFO9608568.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            complement(1662..2618)
                     /locus_tag="ACHLIF_004718"
     CDS             complement(1662..2618)
                     /locus_tag="ACHLIF_004718"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO9608569.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            complement(2637..3674)
                     /locus_tag="ACHLIF_004719"
     CDS             complement(2637..3674)
                     /locus_tag="ACHLIF_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO9608570.1"
                     /translation="MVLIKKNVLSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIE
                     FFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSL
                     QYNANRTYFKTIRKATLGYEQKIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQ
                     DNIYVHAFYRF"
     gene            3947..5179
                     /locus_tag="ACHLIF_004720"
     CDS             3947..5179
                     /locus_tag="ACHLIF_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9608571.1"
                     /translation="MRLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLIWSDATSA
                     EYMKYAATEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPKALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMTNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            5182..>6389
                     /locus_tag="ACHLIF_004721"
     CDS             5182..>6389
                     /locus_tag="ACHLIF_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9608572.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFLASIAFIVLPIVITVLVSFTNY
                     AAPNHIPPKNLVDWVGIKNFIMLFKFKIWSDTFLGVALWTFIWAICATIFTFSFGFIL
                     ALALAKKNIRFAKVWRLIFILPYAIPTFVTLLIFRLLLNGIGPVNSFLNSIGMNSVDF
                     LSVPLNAKITVIAVCVWVGAPYFMLLIAGALTNISRDLYEASEVDGATKWQQFKEITL
                     PMVLHQVAPTLVMTFAYNFNNFGAIYLLTEGGPTNPEYRFAGHTDIFITWIYKLTLNF
                     QQYHIASVIS"
BASE COUNT         1869 a         1268 c         1191 g         2061 t
ORIGIN      
        1 taaactcttt gttgttaacg gtaaagtaaa gatatttttc actgcccata ttttcaataa
       61 tacttaatgc accagtgact gtattcattt ctttgcaatt caaactcaaa gagatatgtt
      121 ctggacgaat accaaaacaa acgggtttat ctatatacgc tttgagtact gattgtttat
      181 tctcaggtat gtacaaccga atatttttgg caatgtcaat atagagttta ccgtcttcct
      241 cacgtacaac cgcatctatc aaattcattg acggggaacc aataaagcca gcaacgaatc
      301 tgttagcagg gtggttatac agattaactg gtgtgtcgac ctgcattatt cgcccctgat
      361 taagcacaca aatacgatca ccgagggtca atgcttcaat ttgatcgtgt gtaacataaa
      421 tcatggttgc tggatgaccc tcatccttga gagactgatg caactgagca attttgactc
      481 gcattgaaac acgaagtttt gcatcaaggt tagatagtgg ctcatcaaat aagaaaacat
      541 ctggcttacg gacaattgca cggcctacag cgacacgctg acgctgacca cccgacattt
      601 ctttcggttt tcggtaaagt aaatcgctaa tttcaagttt ttctgcaact tcttcgacaa
      661 ttttcttaat ctcttctttt ggcctttttt gcatttttaa accaaaagcc atattgtcaa
      721 atacagtctt atggggatac agcgcatagt tctggaacac cattgcaatg ctgcgatctt
      781 taggcggaag ggtattaaca aggcggtcgc taatgtaaat ttcaccacct gatacgtctt
      841 caagacctgc aatcatgcgt aatgtagttg atttggcaca cccagatggt ccaacaaaaa
      901 ccatgaattc accatcgttt atatcgagat caatgccgtg aacagctttg aatccattgt
      961 cataaatttt ttcaactttg cgtagaatta ccgcggccat aatctgctct cattaacaag
     1021 aattttggaa tactgatact ccactattca acgaatgttg aatagtggag tgctaaaaat
     1081 aactatttac atggtgctac catggcggct attgcctttt tatcaggacc aatttgaatc
     1141 gtccaaacat actttccatc ggcaggaata acgacttttg tatttttatc catgcctcct
     1201 ttcttgagtg ttttagcctg atttaatggc aattcaagac ctgttacagt gaactgcggg
     1261 ttccagttca tagtcgcaaa ttgcattgtt accgcacctg ctttttcaga ataaataagc
     1321 tgataaatac catctccctt ataatgcatt ttacgctctg gcttcattaa ccaatagcct
     1381 tccgggaggg ttccggaaat gtaaagaccc gcagcaattg gccctctttc atttacatca
     1441 gggaggttac attctgaata gcgaggatcc gctttcacga caccttcctg actgccagag
     1501 ggtgaattgc catcaacgca tcctgaaaga agaccagtca acacagccgc aataagcaat
     1561 ttatttttca tttaaactct caataaaagt agtatagctt agttaagcta tttctatgtc
     1621 agtgttaatg ttaatgtata taaataatac ttctcaacga ctcactgttt atatacacca
     1681 tgctgtttag ttagtttatt aaggacagcg agatttttgt tgtattctcg cgtatttttg
     1741 gcagggtaac caaaaaggtc actcttttca ggacaagaat ggcttacacc tgatttagcc
     1801 ttaattaccg aataactacc aattgatatg tgaccggcga ttccgacttg accatgtgta
     1861 ataacatggt cgcctaaaac tacgtgtcct gaaaaaccgc attgtgaaac aatcaggcat
     1921 ttattaccga ttatgcagtc atgtccaatc tgcacttgat tatcaatacg agtgccttgg
     1981 cctataatcg tatctccaag tgtccctcgg tcaatggtgt tgttacaacc gatttcaaca
     2041 tcatcaccta tgattactct accgatgcta tcaactcgga catatgaatc acgttcatcc
     2101 gacatatatt caaaactata attaccgatg gagttattag aatctatgat aacgtcattg
     2161 ccaataatag taccttcttt aatcactgta ttcgcatgaa tggcaacatt atcacctatg
     2221 gtcacacagt tcataatttt aacaccaggc ataaaatggc agttcatacc tatctggcag
     2281 tgtttaccaa tgtatacatc aggtatcgtt gaagtgtttt cttgctcaaa caaacgatac
     2341 ttatgtacct taaaatatct taaaactttg ttaagattat tcacgttcag gtaatcgact
     2401 acaatctttg ccttagcatt agaactaatg atctcttccg gtccgataat tatatccgca
     2461 ccagtcgttg gtatatgcaa caaatctcca ggtgtcataa caatcgagag tccaccaact
     2521 aaaggactat ggattggccg aatggtatca accctgagtg atggatcacc atataaagtt
     2581 ccttgcatca acgttttaat atctagtgcg ctgaacatag gtttccctct gtttaatcaa
     2641 aagcgataga aagcatgaac ataaatatta tcctgattga gcacattata tttttcttca
     2701 tctcttggat cataaaagtt tttgtgctct ttttcccagg catgtttata ttcaatcttg
     2761 gctatccagt cgtcacctat tttttgttca taacctagag tggcttttcg aattgttttg
     2821 aaataggttc tgtttgcatt gtattgcaaa ctatctgcgt tttctgtttt cccgtgtccg
     2881 aagcgggtgc gtagatacag attacctgcg ttttcaaaag catatttgat aattggctcg
     2941 tagtaagttt cggtataaat actacgatca tttattttac cgtgattaaa ttcatcgtta
     3001 tgctttttct ggtagaaaaa ttcaatacca aactcccatg caccaaaacg tttatagggc
     3061 tgaaatagta tactttgacc ttcttcttcg cgtacgcccc aggagctctt atcatttcga
     3121 ctgtataagt attcaacgtt tgaataaaaa cctgcgttcc agtcagagtt aaagtacgtc
     3181 aacgtagggc gaaaactgtt gtcaacagtt acagtatgca agcctgtaac ataagggcta
     3241 tctatatttc cgtcggtata catcaggtcg tataccagac tgaagtcgaa accgttacca
     3301 atagtgaacg ttttgtttag tgatatatag ttttgcagac ttgtttcatt ttcgtgatag
     3361 cctttttctt cttgccggcg atcgatagac ttaaggccat agattgctgc aaattgccaa
     3421 ttaggttggc tgaaataggc ctggaaaatc tcgtgtgttt tagtatattc ctttactgtt
     3481 ttacctaaat agttatcagt gcttttggtc tcatattcaa atgtagtacc aacatgtcca
     3541 ttaaagcccg cttgttcttc tgaccagtca gtgtcggagg taaagccgcg atcgtttgcc
     3601 gcaaaggctc ctgttgagcc taaaaggact gttgcaattg aagccgataa gacattcttt
     3661 tttattaaaa ccataaatga gttccttcca aaatatcaat ttaagatatg atttctgtta
     3721 ttccatatag tcgtacctca tgtactccta aacactctgc ggcaataaaa tatttttttc
     3781 ctcaacaaat tactgcaaat gagatatgca tcactatttg cagttattca tcattaaaat
     3841 cttatttgac agttgattgg ccttgatttg tgttgataat ggcgtgaatg cttatgtagg
     3901 cgtacagcat gtactactta aagatattat tcacgaggta aatataatga gactttcaaa
     3961 tatcgtcaca gttataattc ttgcaatttc cagtacgcta acaccccagg cgatggcaga
     4021 aaaattgatt cctgaaactg atgctgagtt attaatctgg tctgatgcca caagcgcgga
     4081 atatatgaaa tatgcagcaa cggaattcaa taaagatttt ggctacaagg tcaagtttac
     4141 atttcgcaat atagcgccaa tggatgcagc atcaagaatt atgcaggatg ggggtacgac
     4201 tcgtgtagct gatgtagctg aaattgaaca tgataccctg gggcggttag tcgttgctgg
     4261 cggggttatg gaaaacatgg tctcagctga gcggattaaa aaaacattta ttccaggcgc
     4321 agtatcggca gctacatata ataacatcag ctatggtttt cctgtaagtt tcgcaacgct
     4381 ggcgcttttc tataataagg atttgttaaa caccgcacca aaaacattcg aagaaatcaa
     4441 tactttcagt gaaaagttta ataattcatc cgagcataaa tatgctctgc tatgggatgt
     4501 acaaaattat tatgtttcac gtatgtttat taccttgtat ggtgccaacg aattcggtaa
     4561 aaccggtaac gatcctaaag ctctaggcat cgcttcatct gaagcgaaga aagggctaga
     4621 gacaatgaaa cgcttaaaga aagcgaatcc ttctaatcct cttgatatgg gtaatccaca
     4681 agttctaaga ggtctgttta atgaaggaaa agttgctgct gtaatcgacg gaccttggtc
     4741 catacaaggt tacattgaca gcggaatcaa ttttggcgtg acacgcatcc caacattaga
     4801 tggtcatcag cctcgcacat tttcaacagt acgactggcc gttgtaagct catttaccga
     4861 atatcctcat gccgctgaat tattcgctga ctacctgaca acggataaga tgttgatgaa
     4921 gcgttatgaa atgacgaatc tcatcccacc aatcgattca cttatgaaca aaattagcca
     4981 gacaggtagt gaagctataa aagcaattat tgctcaagct aattattctg acgcaatgcc
     5041 atcaataccg gaaatgtctt atctatggtc tccaatgact aatgctattt tggctacctg
     5101 ggttgagaat aaaacaccgg atgaagtttt aaatcatgca caaacaatta ttgaagaaca
     5161 actttcgctt caggagtaat tgtgaataca caacaactga aaatgaagag tgcacctgtg
     5221 ctacctattt cctgtttgat tatgggcggg actcagttat cacgtcatta ttatgtgaaa
     5281 ggcgggatat tctttgcaat acaagtatgc tttttgcttt atctttccga tattgtgcat
     5341 acattaatag gtttgttcac attaggtgat gtcgcgcaaa ttcgaaaagg gttgactgtt
     5401 attcagggtg acaattctat atttatgctt gtagagggcg tgatagcgac cattattgtt
     5461 ggcttattcg ccactattta catccttaat attaaagatg cgcggaactc atcttattgc
     5521 catctgactt ttaaacaaca gttatataag ctctatgagg ataaatttgc atttatagtc
     5581 ctaacgcccg catttttagc gagtatagca ttcattgttt tacctattgt aattacagtt
     5641 cttgtatcgt ttacaaacta tgctgcgcca aatcacatcc caccaaagaa cctggttgat
     5701 tgggttggga ttaaaaattt cattatgttg tttaaattta aaatatggtc tgatacgttt
     5761 ctaggtgtcg ctctctggac atttatttgg gcaatttgcg cgacgatttt taccttcagt
     5821 tttggcttta ttctggcttt agcactggct aaaaaaaata tacgtttcgc aaaagtctgg
     5881 cgattgatat ttatactgcc atatgccata cccacatttg taacgttact tattttccgt
     5941 cttcttttaa atggaattgg tccggttaac tcgtttctga acagtatcgg aatgaattca
     6001 gttgattttc tgtcggttcc attaaatgca aaaataacag taattgccgt ttgtgtttgg
     6061 gtaggggcac cgtattttat gctattgatc gcgggtgcat taacaaacat atcccgagat
     6121 ttgtatgagg ctagtgaggt cgatggcgca actaaatggc aacagtttaa ggaaattaca
     6181 ttgccaatgg tgttacatca ggttgcgcca actctggtaa tgacatttgc atataatttc
     6241 aataattttg gtgcaattta tcttctgact gaaggtggtc cgactaatcc ggaatacaga
     6301 ttcgctgggc ataccgatat ttttattacc tggatttata aactcacatt gaatttccag
     6361 caatatcata tagcttctgt aatctccat
//
LOCUS       DAZGOB010000199         6300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGOB010000199 DAZGOB010000000
VERSION     DAZGOB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.199"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..78)
                     /locus_tag="ACHLIF_004722"
     CDS             complement(<1..78)
                     /locus_tag="ACHLIF_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608573.1"
                     /translation="MREQIKQDIDLIEILFYLKKKVRVIL"
     gene            complement(291..1961)
                     /gene="betA"
                     /locus_tag="ACHLIF_004723"
     CDS             complement(291..1961)
                     /gene="betA"
                     /locus_tag="ACHLIF_004723"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO9608574.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRI
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1975..3447)
                     /gene="betB"
                     /locus_tag="ACHLIF_004724"
     CDS             complement(1975..3447)
                     /gene="betB"
                     /locus_tag="ACHLIF_004724"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO9608575.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGGGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFSPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3461..4048)
                     /gene="betI"
                     /locus_tag="ACHLIF_004725"
     CDS             complement(3461..4048)
                     /gene="betI"
                     /locus_tag="ACHLIF_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO9608576.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4177..6210
                     /gene="betT"
                     /locus_tag="ACHLIF_004726"
     CDS             4177..6210
                     /gene="betT"
                     /locus_tag="ACHLIF_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO9608577.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1298 a         1733 c         1739 g         1530 t
ORIGIN      
        1 aaggataaca cgaacctttt tcttcagata aaataaaatc tcaatcagat cgatatcctg
       61 tttgatttgt tcacgcataa tatatccaga aaataaaatc tgtcgcagat aaggttgtat
      121 taatagtctg tatcaggaat gttcgggtta aatatcagca aaaagccagc atcatggata
      181 ctggatatga agcatgagag ttacctcggt gtttatataa ggattcggtc cccctctctg
      241 gaacggtaac tctcaatctg atcggttcct gcgttagttc acatcacgac tcattttttc
      301 gctctcaccg gcatcccatt tgccacaaaa tatcccgccg tgctcctcgg cagcgcttcc
      361 tgtccacgaa tcatatccgc tattttctcg ccaatcataa ttgtcgtggc gttcaaattc
      421 ccggtgataa tctgcggcat aatcgacgca tccaccacac gcaggccttc taacccgtgt
      481 acgcggcctt cgccgtcaac cacggacatc tcgtcgtaac ccattttgca ggtaccgcac
      541 ggatggaagg cggtttcggc gtggttacgc acgaactcat cgagctgttc atccgtctga
      601 cattccgtgc cggggctgat ttcgcggcca cgatactgat ccagcgcggg ttgatgcatg
      661 atctcgcggg tgatgcgaat tgcgtcgcgg aactcctgcc agtcctgctc gtgcgacatg
      721 tagttaaaca gaatcgccgg atgctggtgc gggtcgcggg atttaatccg cacatgccca
      781 cggcttggcg agcgcattga gccgacgtgg cactggaaac cgtgctcttt cactgcattc
      841 gagccgttat agttaatcgc tactggcagg aaatggtact gaatattcgg ccacgcaaat
      901 tcctcacggc tgcgaataaa tccacctgct tcaaagtggt tgctggcacc aacgccagtg
      961 ccgccaaaca gccactccgc accgattttc ggctggttcc accactgcag ggcagggtag
     1021 agggaaaccg gttctttgca ctcatattgc agatacatct ccagatgatc ctgaagattt
     1081 tcgccgacgc cgggtaattc atgcaccagc ggaatatcaa actccgccag cagttcagcg
     1141 ttgccgacgc cggagcgttg caggatctgc ggtgaggcaa tcgcgcctgc acataacagc
     1201 acttctttgt tggccgttgc gcgggttggg atggtgctgt cgccttccag ccattcgacg
     1261 cccaccgcgc gtttgccgtc aaaaatgatg tgatcggtca tagcgtgaat acgaatggtc
     1321 aggttagggc gcgatttggc ctgatcgaga taaccgcgcg cggtactggc gcgacggccc
     1381 tgcggcgtga cggtgcgatc catcggacca aaaccttcct gctgataacc gttgagatcg
     1441 tccgtgcgcg ggtagcccgc ctgcacgccc gcttcaatca tcgcttcaaa cagcggattg
     1501 acgccgggtt tggaggtagt gacgctcacc gggccatcgc caccgtggta gtcgttctcg
     1561 cccacatcgc gagtctcggc cttgcggtag tagggcaggc agtcgagata actccagttc
     1621 tccagaccgg gttcttgcgc ccagttatcg agatccagcg cattgccacg gatgtagcac
     1681 atgccgttga tcagcgacga tccacccagg cctttaccgc gtccgcactc catgcggcgg
     1741 ttattcataa acggttcagg ctctgtttca taggcccagt tgtagcgttt gccctgtagt
     1801 gggaacgcca gcgctgcggg catctgggtg cggaagtcaa agcgatagtc cgggccgccc
     1861 gcttcaagca gcagcacgga ggtattcgga tcttcagtca gacgggtagc gagaacgttg
     1921 ccggctgagc cggcaccaat aatgatgtag tcaaattgca aataaacctc ctggttagaa
     1981 tatggactgg aatttagcca tctcaacctg gatggacttc acctgggtgt aactctggag
     2041 cgtcatcacg ccgttctcgc gaccaatgcc ggagtgtttg tagccgccaa cgggcatctc
     2101 tgccggggat tcgccccagg tgttgatcca gcaaataccc gcttccagct gatgaatgac
     2161 gcgatgcgcg cggttcaggt ccgccgtcac gatgcccgcc gccaggccgt agtcggtatc
     2221 attggcgcgg cgaatcactt cgtcctcaga ttcataagtg agaagcgaca tcaccggacc
     2281 gaagatctct tcgcgcacga tggtcatttc gtcgcggcaa tcggtgaaca cggtcggtgc
     2341 aacccatgcg ccgttatcga agccatcgcc tttcagtaca tcgccgccgc acagtacgcg
     2401 cgcgccttcc tctttgcctt tggcgatata gcgcagcacg ttatcgcgat gcgggaagct
     2461 gaccagcggg ctgaagttag tttgcggatc gaaaacgtcg cccgcgcgaa tgcgctcaac
     2521 gcgcgccaga attttctgct caaatgcggc tttgcatttc gccggaacga agacgcgggt
     2581 gccgttggta cacacctgac cggagctgaa gaagttcgcc atcatggcga tatcggcggc
     2641 gagatcgaga tccgcatcgt cgaaaacgat cagcggtgat ttaccgccca gttccatggt
     2701 cacttctttc agggaagagg ccgccgagtt agccatcact tttttgccgc tggcgacacc
     2761 gccggtaaat gacactttgg caatgcccgg atgctcggtc agatattgcc cggtctccgc
     2821 gccccccccc ggcaacacgt taaatacgcc gtccggcagg cccgcttcgc tgtaaatttc
     2881 agccagcttt aacgcggtaa gcggggtgac ttcgctcggt ttgaaaatca ttgcgttgcc
     2941 tgccgccagc gccggggcgg atttccacag ggcaatctgg atcgggtagt tccatgcgcc
     3001 aatccccgcc accacaccca gcggttcgcg gcgggtataa acaaatgacg tctcacgcaa
     3061 cgggatctgg ctgccttcca gcgccgggat cagcccggcg tagtactcca gcacgtccgc
     3121 accggtaacg atatcgacgg ttgaggtttc cgaatatgct tttccggtat cgagggtttc
     3181 cagttttgcg agttcgtcat tgcgttcacg cagaatatca acggcccgac gcagaatacg
     3241 cgagcgctcc atagcggtca tcgccgccca gattttttgc ccctgttggg cactcttcac
     3301 ggcgcgatcg acatcctcac gcccggcggc ctgcacggtc gccagcacgt taccgttggc
     3361 cgggttaatg gtctcgaagg tgcgaccgct ggtggcggag gtataaccac catgtatata
     3421 aagctgctgt tctgccattc gggacatcac gtctcctcgg ttaatcggtg ggtaaatgct
     3481 gagtaataaa gtgacgggtc agcgagtgtg cgagaggttt atccagcggt ttgccgctca
     3541 gtgccgcgcg cagccataat ccgtcaatca gcgcggcaag gccgtagccc gcttcctgtg
     3601 cctgttggcg cggtaactcg cggcgaaatt cgctcaccag attcgacaat aagcggcggc
     3661 tactgacctg ctgtaaacga tagagcatcg gctgatgcat actgctggcc cagaacgcca
     3721 gccaggcttt catcgccgca ctgctcacct gcgtttcatc gaagtttccg ccaacaatcg
     3781 cctgtaatcg ctgctctgca ctgccctgcg gaagtgcatg taatcgattc aaaaccgcgt
     3841 cacgcagctg actggtgata tcgcgcatgg ttgcttccag cagaccattt ttgtccctga
     3901 aatagtggct gatgatcccc gtagaaacgc ctgcacggcg ggcgatctgc gcgatcgttg
     3961 catcgtgcat gcccacttca tttattgctt ccagtgtggc gtcgatcagt tgtctgcgcc
     4021 ggatcgactg catccccaat ttgggcattt tcgccactcc attcatcagc gttgtttatc
     4081 tattaaagcg gttattgatt ggacgttcaa tataaaatgt gtcttaattg ttacgaattt
     4141 gattttaaat ggtaacaata acagtgggga tactggatga cagacctttc acacagcagg
     4201 gaaaaggaca aaatcaatcc ggtggtgttt tacacctccg ccggactgat tttgttgttt
     4261 tccctgacaa cgatcctgtt tcgcgacttc tcggccctgt ggattggccg cacgctggac
     4321 tgggtttcta aaaccttcgg ttggtactat ctactggcgg caacgctcta tattgtcttt
     4381 gtggtctgta tcgcttgttc gcgttttggt tcggtgaagc tcgggccaga acaatccaaa
     4441 ccggaattca gcctgctgag ttgggcggcg atgctgtttg ctgccgggat cggtatcgac
     4501 ctgatgttct tctccgtagc cgaaccggta acgcagtata tgcagccgcc ggaaggcgcg
     4561 ggacagacga ttgaggccgc gcgtcaggcg atggtctgga cgctgtttca ctacggctta
     4621 accggctggt cgatgtatgc gctgatgggc atggcgctcg gatactttag ctatcgttat
     4681 aatttgccgc tcaccatccg ctcggcgctg tacccgatct tcggtaaacg gattaacggg
     4741 ccgatagggc actcagtgga tattgcagcg gtgatcggca ccatcttcgg tattgccact
     4801 acgctcggta tcggtgtggt gcagcttaac tatggcttga gcgtactgtt tgatattccc
     4861 gattcgatgg cggcaaaagc ggcactgatc gccttgtcgg tgataatcgc cacgatctct
     4921 gtcacctccg gtgtcgataa ggggattcgt gtgttatcgg aacttaacgt tgcgctggcg
     4981 ctgggattga tcctgttcgt attgtttatg ggcgacacct cgttcctgct taatgcgctg
     5041 gtgctgaatg ttggcgacta tgtgaatcgc tttatgggca tgacgctcaa cagttttgcc
     5101 ttcgaccgtc cagttgagtg gatgaataac tggacgctct tcttctgggc atggtgggtg
     5161 gcatggtcgc cgtttgtcgg cttgttcctg gcgcgtatct cgcgtgggcg taccattcgc
     5221 cagttcgtgc tgggcacgtt gattattccg tttaccttca cgctgttatg gctctcggtg
     5281 ttcggcaata gcgcgctgta tgaaatcatc cacggcggcg cggcatttgc cgaggaagcg
     5341 atggtccatc cggagcgcgg cttctacagc ctgctggcgc agtatccggc gtttaccttt
     5401 agcgcctccg tcgccaccat tactggcctg ctgttttatg tgacctcggc ggactccggt
     5461 gcgctggtgc tggggaattt cacctcgcag cttaaagata tcaacagcga cgcccccggc
     5521 tggctgcgcg tcttctggtc ggtggcgatt ggcctgctga cgctcggcat gctgatgacc
     5581 aacgggatat ccgcgctgca aaacaccacg gtgattatgg ggctgccgtt cagctttgtg
     5641 atcttcttcg tgatggcggg gttgtataaa tctctgaagg tagaagatta ccgccgtgaa
     5701 agtgccaacc gcgataccgc accgcgaccg ctggggcttc aggatcgcct gagctggaaa
     5761 aaacgtctct cgcgcctgat gaattatccg ggcacgcgtt acactaaaca gatgatggag
     5821 acggtctgtt acccggcaat ggaagaagtg gcgcaggagc tgcggttgcg cggcgcatac
     5881 gtggagctaa aaagcctgcc gccggaagag ggacaacagt tggggcatct ggatttgttg
     5941 gtgcatatgg gcgaagaaca aaactttgtc tatcagattt ggccgcagca atattcggtg
     6001 ccgggcttta cctaccgcgc acgtagcggt aaatcgacct actaccggct ggaaaccttc
     6061 ctgttagaag gcagccaggg caacgacctg atggactaca gcaaagagca ggtgatcacc
     6121 gatattcttg accagtacga gcggcacctt aactttattc atctccatcg tgaagcgccg
     6181 ggccatagcg tgatgttccc ggacgcgtga ttgtttttgc ataaaaccgc gccatgtctg
     6241 catatggcgc gggaatcatt gactcgctgt aaaaaattat gcatatatta tatatgattg
//
LOCUS       DAZGOB010000200         6229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGOB010000200 DAZGOB010000000
VERSION     DAZGOB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.200"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(22..195)
                     /locus_tag="ACHLIF_004727"
     CDS             complement(22..195)
                     /locus_tag="ACHLIF_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608578.1"
                     /translation="MSTPLEKRSPPYQMRLPEDFRAQLEEAAKADGDTSLATWIKRVL
                     RKELQQRGIEPKG"
     gene            326..664
                     /locus_tag="ACHLIF_004728"
                     /pseudo
     CDS             326..664
                     /locus_tag="ACHLIF_004728"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            695..886
                     /locus_tag="ACHLIF_004729"
     CDS             695..886
                     /locus_tag="ACHLIF_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9608579.1"
                     /translation="MATIPTLVHSQTAFLWRFIIFGASEHQIIHVTAWTEREARSRCP
                     SGCVAVFAARIRQEVCHVQ"
     gene            876..1097
                     /locus_tag="ACHLIF_004730"
     CDS             876..1097
                     /locus_tag="ACHLIF_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608580.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            1090..1311
                     /locus_tag="ACHLIF_004731"
     CDS             1090..1311
                     /locus_tag="ACHLIF_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608581.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSISEIMRVAFFFLCTDEHR
                     DTGLNILDIAANYADFVTEAVLRKTTDGD"
     gene            1313..1546
                     /locus_tag="ACHLIF_004732"
     CDS             1313..1546
                     /locus_tag="ACHLIF_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608582.1"
                     /translation="MRDIYHETIDRAFSALAYAEGMYEILRIWLETLGDNERDKQKSR
                     IVTALITLLEPVINELQEIETLHDRYNEQHTGE"
     gene            1552..1851
                     /locus_tag="ACHLIF_004733"
     CDS             1552..1851
                     /locus_tag="ACHLIF_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9608583.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            1848..3248
                     /locus_tag="ACHLIF_004734"
     CDS             1848..3248
                     /locus_tag="ACHLIF_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO9608584.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSQKRENWREYLERERGKKPGEEKHHQRKTTLPMSVGSAGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHGEQVKNLYLVNRPIFPAAPLDVEEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPRPPV
                     ELERIEEAGNKTENHAALWQCIRTRTAHGEACTIALLRDDMKKLGYEMKHFRRWLYKL
                     ENDGVIVIDGDDVRPL"
     gene            3450..3695
                     /locus_tag="ACHLIF_004735"
     CDS             3450..3695
                     /locus_tag="ACHLIF_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608585.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
     gene            3826..4020
                     /locus_tag="ACHLIF_004736"
     CDS             3826..4020
                     /locus_tag="ACHLIF_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608586.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
     gene            4024..4185
                     /locus_tag="ACHLIF_004737"
     CDS             4024..4185
                     /locus_tag="ACHLIF_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFO9608587.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            4313..4801
                     /locus_tag="ACHLIF_004738"
     CDS             4313..4801
                     /locus_tag="ACHLIF_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9608588.1"
                     /translation="MALTTKKKRFADALSKGTNPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAANVSSNRFPIPKPPTGL
                     KH"
     gene            4813..4974
                     /locus_tag="ACHLIF_004739"
     CDS             4813..4974
                     /locus_tag="ACHLIF_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608589.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQPTEIRLQAAAVLMPYFHETLTPC
                     YQEEEDNDF"
     gene            4964..5887
                     /locus_tag="ACHLIF_004740"
     CDS             4964..5887
                     /locus_tag="ACHLIF_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO9608590.1"
                     /translation="MISKNMKGVSAVRAASVLTSCKFSHADAIEMTKKNDPAVSKFLE
                     KSIVAGMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLATSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGTV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
BASE COUNT         1815 a         1393 c         1621 g         1400 t
ORIGIN      
        1 acaccacggc gctgatagtt tttatccttt gggttctatg ccgcgttgct gtaattcttt
       61 gcggagaacg cgtttaatcc aagtggctaa agatgtgtcg ccatcggcct tagctgcttc
      121 ttctagctgc gctctgaagt cttccggtaa tcgcatttgg tatggaggcg aacgtttttc
      181 taatggtgtt gacatggtaa ttacccttca ttactatacc tacatggtaa ttaccattgt
      241 acttgccatt acacaaaaat caacgccccg cagtgctggg aacacatacg gggcgtctaa
      301 ccaacaacgt aaactaggag ccgttatggt tgccgtaaat catataccac accttgtaca
      361 cacacaaacg gcctttgtgt ggcgttttct ggccctgaat atcggagaaa aaaaccagtt
      421 gatcgccagt gtcgaaaata gctatgattc ccgcgcacct cataaaacgg gtgccgggat
      481 tggcgtcctg ggcaaaactg aagcgcataa cacgcgcccc gcgtgttttt ttgtgccgca
      541 tagtcacacc ttatcaatgg tgggctgtac gggggcggag caatccgcgc cggtttcttc
      601 agtgtccggt tacgccaacc ctgtacagtc cgccaccagc gaaattggcg tttccggtgg
      661 cggttatctt aaccacactg aggaggctgc caacatggct actatcccta cccttgtaca
      721 ttctcaaacc gcctttctct ggcgctttat catctttggc gcgtcagaac atcaaatcat
      781 ccacgtaacc gcctggacgg aacgcgaagc gcgtagccgt tgcccgtccg gttgtgttgc
      841 tgtattcgcc gcccgtattc gtcaggaggt gtgccatgtg caataaaacc acaccggacg
      901 cagcagccgc cgcgctcact acgctgatgc acgcgcttat cgatattgaa tgcactgcag
      961 agcttgcgca gggagaagaa cagaaagacc ggacacagtt cgccctggaa tgtatccgat
     1021 acatcgcaac gcggtcgctg aatgacgcta aaaatattct tgttgctgat tgtgaaaatg
     1081 gggggggtta tgcgtgatga tcgttttaat tccctgaaac aagaattttc cggcgttcct
     1141 gatgatgcgg ctgatgcgct ttcgtcaata tctgaaatta tgcgggtggc ttttttcttt
     1201 ctttgcactg atgagcacag agatacaggg ctaaatattc ttgatattgc cgctaactat
     1261 gctgatttcg tgacagaagc tgttttaaga aaaacaacgg acggggatta atatgcgtga
     1321 tatttaccac gaaacaatag accgcgcatt tagtgccctt gcttacgctg aaggtatgta
     1381 cgaaatattg cgcatatggc ttgaaacact tggcgacaat gaacgcgaca aacaaaaatc
     1441 aagaattgtc acggcattaa taacgcttct tgagcctgta ataaatgagt tgcaggaaat
     1501 agagacattg cacgacagat ataacgaaca gcacaccgga gaataaaaat aatgaaactt
     1561 aaatattctg gcttaactgc cagtggcaac actcacccta aatttacgcg cggtgatatt
     1621 taccgcgacc agtacggcgg cacggtaatg attaagggcg tggcgggacg gtgcgtaact
     1681 taccgccgtg aaggttacga atatgattgc gtgatgcctg tttatcagtt ccggcgtgat
     1741 ttttctctgg tacagaccgc gccgcataac gtgcccacca gcaacgccag ggcacgggca
     1801 aacatccaga agctgaaaac catgattaac ggattcaggg gcaagaaatg aaaagcgcac
     1861 cgaacttaaa aaaacagcct tacgacaaga tgaccgaagt cattattttt gcgggtagtg
     1921 atgcctgggc acatgcgaaa cagtggcagg aacaggacgg gcgactggct ggcgataatg
     1981 tgcctcccgt tgtgctggct gatgatcaac tggatgaact ggcagacctg agaatcatcg
     2041 acgaggggcg ctattgtgtc cggctgtaca aggcaggcca catcaggcca tcaaatatta
     2101 atgccattgc gcacaagctg gcggcggcgg gtgtaactga tgcgaattat taccccgaag
     2161 ggatgcacag ccagaagcgg gagaactggc gcgaatatct ggaacgggag cgggggaaaa
     2221 agccagggga agaaaaacac caccagcgaa aaaccacgct accaatgagc gtgggatctg
     2281 ccggatacga cacacaactt gattacgtgg ttaaggggat tattccggcg gtatcgctgt
     2341 gcagcatata cggggcgagc ggttcatata aatcgttcct tgcgggttcg tgggcgtgtc
     2401 acgtttccac gggccgccag tggggaggcc gcagggtggc gcatggcgct gttctctatg
     2461 tggttggtga aggcggtatc ggcgttccgc gtcgtgtaaa agcctgggag gttgtgcacg
     2521 gtgagcaggt gaaaaatctg tatctggtaa accgtcccat ctttccggct gccccgcttg
     2581 atgttgagga aatggttatc gctgcccgtc aggtggaacg ggaaacgggt aaaccggttc
     2641 gaatgattat tctggatacg ctggcgcgct gctttggcgg caatgatgaa aatgattccc
     2701 gtgatatggg ggcgtttatc cgtgggtgtg acgaactgaa acggcgcaca ggggcaacgg
     2761 tgctggtggt tcaccattcc ggcaaggatg aaacaaaggg ggcgcgtggt tccagtgcat
     2821 ttcgtgcatc actggacgcc gaataccgga tacgcaggga ggacgcaggc agcgaagcgc
     2881 tggttatctc atgtaccaaa atgaaggacg cggaggaact caaagaagca gcatatgact
     2941 tacgcgtggt ggagcttttt accgacgctg acggggagtt aatcacgtcg ctggtggtgg
     3001 tggataagcc gcgccctccc gttgaactgg agcgcatcga ggaggcaggg aacaagacgg
     3061 agaatcacgc cgcgctatgg cagtgcatca gaacacgaac agcacacggg gaggcgtgca
     3121 ctatcgccct tctccgcgac gatatgaaaa agctggggta tgagatgaag cacttccggc
     3181 gctggctgta caagctggaa aatgatggcg taatcgttat tgacggtgat gacgtgcgcc
     3241 cgctgtaaaa agtggggagt aaaagtgggg ggagtgggga atttaacaaa attgaaacat
     3301 aattacccac tttcccacct gtatataccc aaaaaagtgg ggagtaaaaa acatattgaa
     3361 aaacataacg ttagaatcac aaaaaaaaga ggtggggaga cggtgggtaa tttcaaaaag
     3421 tggggagcaa aagtggggag tagtggggaa tgaacagaaa aaccagagat aagacagcgc
     3481 caaaatataa agcgttagac atgacagagc tttccttaaa ggtggcaatc agaacgatag
     3541 accgccacgc gggggaagga tacgcgaagg agcatcccga actgataagc gcattcatga
     3601 ccacagcggc ggcaaacttt gccacgctga cagaacggga gattgccgaa gcggaacagg
     3661 taacaaccat caacgttaaa agcgctggag aataaccgaa gaagtgagca catagccgga
     3721 gcaatccggc tttttttcga tctcatatat cttcaaagat ggttaattaa tgataatcta
     3781 tcgaaaatac tggttatgca atgataagta tcaggagaaa acaaagtgga agagataaag
     3841 caaagcgcta ccgtgatgat tgatcatgaa atccttaacg agcttaaaaa gttgcagttc
     3901 gatattgtgc gccggatgaa tgaatcaggt atctacggca gggcggcaac accaacaata
     3961 gggcatcttg cgcgcgtact gttacgcgaa cgcctgggca ttgcaacaag tgaggcgtaa
     4021 acaatggcta tcacagaacg cgaggcaagc atcattaaag ccattggcga ggaaacaaga
     4081 gacaccatag cgccattgat ggaaaagatc gcagagcttg agacacgaat aaacaagctg
     4141 agtgatgaag ttgaaggcct gatctcgtta ctcggtgagc aatgacaggg ggggagtgtt
     4201 ccggccggaa agtcgatcgc cctggacacc gcccccagtc tcattcatag aaaattttgc
     4261 ttttcaaaaa agttacacga aaagttacac cccctcaaaa cgaggttttt gcatggcatt
     4321 aacgaccaaa aaaaagcggt ttgctgatgc gttatcaaag ggcacaaatc caacggaagc
     4381 cgcgatacat gcgggataca gcgaaaagac tgcaagggtt aaaggctggc agctatccaa
     4441 tgatccggag gtgaaaaagt atcttaaggc tgtaacttct gatgtaactt ttcaggttac
     4501 aaaaccgaaa aaagttacaa agaaagttac cgaaaaagtt acaccggaac gggcatcaat
     4561 taacaccatt gagatgatgg ataacggcct acccgacccc atcaaagcaa tgggcaggat
     4621 actgattgac aacatggaca cagatccaaa actggcttta gatgccgcgt ttaagctggc
     4681 ccagttcacc caccgcaaga tcggcagcat tgggaaaaaa gaggttaaaa aactcaatgc
     4741 cgctaatgtc tcctcaaatc ggttccccat ccctaagcca ccaactggct taaaacactg
     4801 aggtagcgaa ctatgagcga atacatcaca gaccctattg atgtactcac ggaaatcatg
     4861 aacgacaagc agcagccaac cgaaatacgg ttacaggctg ccgctgttct gatgccatat
     4921 tttcacgaaa ccctaacccc atgttaccag gaggaagaag ataatgattt ctaaaaacat
     4981 gaaaggcgtg tcggcagttc gcgccgccag cgtgcttaca tcgtgtaaat tcagccatgc
     5041 agacgcaatt gaaatgacga aaaaaaatga tccggcagta tcgaaatttc ttgaaaaatc
     5101 cattgttgcc ggaatgacta cggataatat gccgtcgctg gtggcagagc cgatagtaag
     5161 agagcttggc gagtatattt tttcgcagtc tgtaccaggc aagctaatca gcagcgcgct
     5221 gaatcttccg tttggcgcaa aattgcagtc aatatcaggc aagggtagta aatggcttaa
     5281 agaagccgaa gaaatccctg tcaaagaggg agaagtaaac gaaagtgcgc taaaactgtg
     5341 caaacttggc gctattgtga tgctgaataa cgagctaatc aaatttagca cccctggtac
     5401 caattcagcg atcagagact tgatcacatc ggaatcggtg aaagaaatag ataaaaaatt
     5461 tctttccagt gaccaggagg tggcgggcgt atcccctgag ggcgtgctta atggtgctga
     5521 tgaggccgaa aacttcatag agcttttcaa aaagcacata gcgaacggta acacgcttgc
     5581 cacgtcgtcg ctaatcttgc ccgtagaaaa cgccttacag cttactgatg ctgagttctg
     5641 gcagatagag cttttgagaa taccggtaat tgtgagcgaa caggcggatc gcatgatgct
     5701 gatagacgct caaaagctga ttattaacgt tgaagccacg gttataaccc caactgatga
     5761 ggcggtaata aaaaccgaat cagggacggt tagccttttc cagaatgacg ctacagcgtt
     5821 cagggcaatc acctattgcg gatggcaaaa actcgataag gccgtcaccg ttctggcgaa
     5881 aagctgattt ttccggcatc aatcccgata tggtgatccc catatcggga taattacgaa
     5941 attttcgcag tttaattaat ggtcagatta atcgctaacc ctctgatatt ttcggaaacc
     6001 tcaatttgag gaagtcggaa gcgtcaccct tcaaatcctg aatggtcata atgaccacgc
     6061 agaccatcaa ggcaaccata acgaacatcg ttacggttgg agcttttacc acaggaaaac
     6121 tcgcatttaa attgtacact ttttcagcaa atgattttag ttatacgttt agttatacgg
     6181 tttaaaagtt gaatcaagca tactatttaa aatcatgtag ttacatact
//
LOCUS       DAZGOB010000201         6156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGOB010000201 DAZGOB010000000
VERSION     DAZGOB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.201"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..165
                     /gene="kil"
                     /locus_tag="ACHLIF_004741"
     CDS             <1..165
                     /gene="kil"
                     /locus_tag="ACHLIF_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO9608591.1"
                     /translation="DYVLHEGRTYIASANNIKKRKLYIRNLTTKTCISDCMIKVFLGR
                     DGLPVKAESW"
     gene            165..335
                     /locus_tag="ACHLIF_004742"
     CDS             165..335
                     /locus_tag="ACHLIF_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO9608592.1"
                     /translation="MTKKIKCAYHLCNKGIEESKSIKRPLHFMRGVIPTTEMKKYCSE
                     NCAEKDQMAHEL"
     gene            409..684
                     /locus_tag="ACHLIF_004743"
     CDS             409..684
                     /locus_tag="ACHLIF_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608593.1"
                     /translation="MITNYEVTVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVTKMSLVHIGKNLPAEKKAGFLATLIAMKLKGEI"
     gene            783..3434
                     /gene="recE"
                     /locus_tag="ACHLIF_004744"
     CDS             783..3434
                     /gene="recE"
                     /locus_tag="ACHLIF_004744"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO9608594.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQE
                     DAPETEEPTGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRSMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLARDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEGKQDDEEKPQPSGAMADEQATAETVEPNATEHHQNTQPLDAQSQVNSVDAKY
                     QELRAELHEARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQN
                     QPETEKISPDAKQPEPVVQQEPEIVCNACGQTGGDNCPDCGAVMGDATYQETFGEENQ
                     VEAKEKDPEEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNEN
                     YHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRF
                     IVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQRVMALPLGQWLVE
                     SAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQ
                     DAFYSDGYEAQFGVLPTFVFLVASTTVECGRYPVEIFMMGEEAKLAGQQEYHRNLRTL
                     ADCLNTDEWPAIKTLSLPRWAKEYAND"
     gene            3427..4236
                     /gene="recT"
                     /locus_tag="ACHLIF_004745"
     CDS             3427..4236
                     /gene="recT"
                     /locus_tag="ACHLIF_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO9608595.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            4293..4487
                     /gene="ralR"
                     /locus_tag="ACHLIF_004746"
     CDS             4293..4487
                     /gene="ralR"
                     /locus_tag="ACHLIF_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO9608596.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            4480..4668
                     /locus_tag="ACHLIF_004747"
     CDS             4480..4668
                     /locus_tag="ACHLIF_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO9608597.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            4775..5056
                     /locus_tag="ACHLIF_004748"
     CDS             4775..5056
                     /locus_tag="ACHLIF_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9608598.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFT"
     gene            5022..6098
                     /locus_tag="ACHLIF_004749"
     CDS             5022..6098
                     /locus_tag="ACHLIF_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9608599.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPTWFPLAMDLALVTGQRREDITN
                     MRFSEIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVEQCRMVSRGDYLISAGI
                     RKNSPDGSLHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT         1974 a         1350 c         1448 g         1384 t
ORIGIN      
        1 gattatgttc ttcatgaagg ccggacatat atcgcctcgg caaacaatat taaaaagcga
       61 aaactttata ttcgtaacct gactacaaaa acatgcattt ctgactgcat gattaaagtc
      121 ttcctcggtc gtgatggttt acctgtaaag gcggagtcat ggtgatgact aagaaaataa
      181 aatgtgctta ccacctttgc aataaaggaa ttgaagaaag caaaagcatt aaaagaccac
      241 ttcatttcat gcgtggagtt atcccaacga cggaaatgaa aaaatattgt agtgaaaatt
      301 gtgccgaaaa agaccagatg gcacacgaac tttaattaac tgactatgcg aaactgaatt
      361 tatgccagca atggcaggga ttcgctcaac cttaattaag gagaaaaaat gattaccaat
      421 tatgaagtca ctgttgtaac taccgatgac attgttcacg aggttaatct ggaaggaaag
      481 cgtattggct acgtgattaa aacagaaaat aaagaaaccc cattcactgt ggttgatatt
      541 gacggtccat caggcaacgt aaaaacactt gatgaaggtg tcacaaaaat gagtctggtt
      601 cacatcggaa agaatctgcc cgcagaaaaa aaagccggat ttctggcaac tctgattgca
      661 atgaaattaa aaggtgaaat ctgaaaaaag aaagcctgca caacgtgcag gcctgagtga
      721 agaacctggg acatttattc atcactcgca gtaattttaa tctgagttga ggttaaaaaa
      781 caatgagcac aaaaccactc ttccttttac ggaaagcgaa aaaatcatcc ggtgaacctg
      841 acgtcgtcct gtgggcaagc gacgattttg aatcaacctg tgccactctg gactacctga
      901 tcgttaagtc aggtaaaaaa ctgagcaact attttaaagc tgttgccaca aattttcctg
      961 tcgttaatga ccttccccct gaaggtgaga tcgattttac ctggagtgaa cgctatcaac
     1021 tcagcaaaga ctccatgacc tgggaactaa aaccgggagc agcgccagac gacgttcacc
     1081 atcaggagga tgcaccggaa accgaagaac cgacgggagg ccaggaagaa aacgcgcagg
     1141 cagacgccca cgaggattgc caggattgcg aagtctctgt agccactttg cggttcacac
     1201 agcgtcttct gcacattttt acgtatgcag ccggggatcg aaaatacctg catcatgcca
     1261 cccgtgaaca acgcaaacac attactgctc ttgagatgga tcaggaaaac agctatgtcc
     1321 agaatctgct gttggccata cgcagcatgg cagaaccgac aactctggat aatgccgccc
     1381 tgctccgcct gactgatgca attaaggcag tgttctctat cacgaaaaaa catcagccct
     1441 atgaatttaa gaatttcatt tcagcctggc tggataccga acacattgat cgcggtcttc
     1501 tgacaaaaga atggcgaaaa gggaatcgtg tttcacgcat cactcgcacg gcttccggtg
     1561 ctaatgctgg cggcgggaac ctcaccgatc gcggcgaagg tttcgtccac gatctgacat
     1621 cactggcgcg cgatgtagcc actggcgtac tggcccgttc aatggacgtg gacatctata
     1681 accttcatcc ggcacacgct aaacgcattg aggaaattat cgctgaaaat aaaccaccct
     1741 tttctgtttt ccgcgacaaa ttcatcacca tgcctggcgg gctggattat tcccgcgcca
     1801 tcgtggttgc gtccgtgaaa gaagcaccaa ttgggatcga ggtcatcccc gcacacgtca
     1861 ctgaatatct gaacaaagta ctgactgaaa ccgatcatgc caaccctgat ccggaaatcg
     1921 tggatattgc ctgcggtcgc tcctcggccc cgatgccgca acgagtaaca gaagaaggaa
     1981 aacaggacga tgaagaaaaa ccacaaccat ctggcgcaat ggcagatgaa caggcaacgg
     2041 ctgaaacagt ggaaccgaat gcaactgaac atcatcagaa cacgcagccg ctggatgctc
     2101 agtcacaggt aaattctgtt gatgcgaaat atcaggaact gcgggcagaa ctccatgaag
     2161 cccggaaaaa cattccatca aaaaatcctg tcgatgccga taaattgctt gctgcatcac
     2221 gtggtgaatt tgttgacgga attagcgacc cgaacgatcc gaaatgggtt aaggggatcc
     2281 agactcgcga ttctgtgtac cagaaccagc cagaaacgga aaaaatcagc ccggatgcga
     2341 aacaacctga gccagtagtg caacaggaac cggaaatagt ctgcaatgcc tgcggtcaga
     2401 ctggcgggga taactgccct gactgtggtg cggtgatggg cgacgcaaca taccaggaaa
     2461 cattcggtga agagaatcag gttgaagcta aggaaaaaga tccggaggaa atggaaggcg
     2521 ctgaacatcc gcacaatgag aatgctggca gcgatccgca tcgcgattgc agtgatgaaa
     2581 ctggcgaagt cgcagatccc gtaatcgtag aagacataga gccaggtatt tattacggaa
     2641 tttcgaatga gaattaccac gcgggtcccg gcgtcagtaa gtctcagctc gatgacattg
     2701 ctgatactcc ggcactgtat ttgtggcgta aaaatgcccc cgtggacact acaaagacaa
     2761 aaacgctcga tttaggaacc gctttccact gccgggtact tgaaccggaa gaattcagta
     2821 accgctttat cgtagcacct gaatttaacc gccgtacaaa cgccggaaaa gaagaagaga
     2881 aagcgtttct gatggaatgc gcaagcacag gaaaaacggt tatcactgcg gaagaaggcc
     2941 ggaaaattga actcatgtat caacgcgtta tggctttgcc gctggggcaa tggcttgttg
     3001 aaagcgccgg acacgctgaa tcatcaattt actgggaaga tccggaaaca ggaattttgt
     3061 gtcggtgccg tccggacaaa attattcctg aatttcactg gatcatggac gtgaaaacca
     3121 cagcggatat tcaacgattc aaaacggctt attacgacta ccgctatcac gttcaggatg
     3181 cattctacag tgacggttat gaagcacagt ttggcgtgct gccaactttc gtttttctgg
     3241 ttgccagcac aactgttgaa tgcggacgtt acccggttga gattttcatg atgggcgaag
     3301 aagcaaaact ggcaggccag caggaatatc accgcaatct gaggaccctg gctgactgcc
     3361 tgaataccga tgaatggcca gctattaaaa cgttatcact gccccgctgg gctaaggagt
     3421 atgcaaatga ctaagcaacc acctatcgca aaagccgatc tgcaaaaaac ccagggaaac
     3481 cgtgcaccag cagcagtaaa aaataacgac gtgatcagct ttattaatca gccatcaatg
     3541 aaagagcaac tggcagcagc tctcccacgc catatgacgg ctgaacgaat gatacgtatc
     3601 gccaccacag aaattcgtaa ggttccggcg ctaggaaact gtgacaccat gagttttgtc
     3661 agtgcgatcg ttcagtgttc acagctcggc cttgagccag gtagcgccct cggccacgca
     3721 tatttactgc cttttggtaa taaaaacgaa aagagcggta aaaagaacgt tcagctaatc
     3781 attggttatc gcggcatgat tgatctggct cgccgttctg gtcaaatcgc cagcctgtca
     3841 gcccgtgttg tccgtgaagg tgacgagttt agttttgaat ttggccttga tgaaaagtta
     3901 atacaccgcc cgggagaaaa cgaagatgca ccagtgaccc acgtctatgc tgtcgcaaga
     3961 ctgaaagacg gagggactca gtttgaagtt atgacgcgca aacagattga actggtgcgc
     4021 agccagagta aggctggtaa taacgggcca tgggtaactc actgggaaga aatggcaaaa
     4081 aaaacggcta ttcgtcgcct gtttaaatac ctgcctgtct caattgaaat ccagcgtgca
     4141 gtatcaatgg atgaaaagga accactgaca atcgatccgg cagactcctc tgtattaacc
     4201 ggggaataca gtgtaatcga taattcagaa gaataattca gcctggcggt gtaatgcacc
     4261 gccaacgtga gacagttttt atgacaaaaa ttatgagata tgacgatgtt aaaccatgtc
     4321 cgttttgtgg ttgtccatct gttacagtga aagcaatttc aggatattac cgggcaaaat
     4381 gcaacggatg cgaatcccga actggctatg gtggaagtga aaaagaagca ctcgaaagat
     4441 ggaataaacg aactactgga aataataatg gaggtgttca tgtataaaat aactgccact
     4501 attgaaaaag aaggtggcac tcctactaac tggacaagat attcaaaatc taaattaacg
     4561 aaatcagaat gcgaaaaaat gctctcaggg aaaaaagaag caggcgtgtc cagagagcag
     4621 aaagtaaagc tgataaattt taattgcgag aaacttctgt cctcgtgagt tgcattatat
     4681 acaaattaga acttcatagc tgattattaa aaatcaacca cacccgccag tattctgtat
     4741 atttactggc ggtcatatcg taagaggtat ggcaatgaat cttgtgacac tcaaaacgtg
     4801 gggaaaactc agatatccgg ataacccacc atcaatatca acgctgagac gatgggcaag
     4861 gaatggaaac atttatcctg cacctgaact acacgggagg agttacaggg tggttccgga
     4921 agctttctat atcaacccaa ataaggttga taccgatata acacaccatc agcctaatgg
     4981 gcgacaaggg agagacagtc cgttactgga gaagttaaaa catgcagcgg aaaaaatacg
     5041 atcccaattt acctaaaaac ttaacatatc gaaggaggga caaagcatat tactggcgca
     5101 accctctgac gaaagaagaa tttacactag gtaaaatttc aagaagagat gcagtagcgc
     5161 aggcaattga agcaaatcat tatatataca aaaactactc tcctgctgcc ttaattgaaa
     5221 aactaaaagg gttcgactca tttactatgg cagactggat tgaacgttac aaaacgattc
     5281 ttataaggag aaaagtgtcc agaaatactt ataaaattcg ggtaaatcaa ctggagacaa
     5341 taaaagaaaa attaggaggg gttttactga cagaaataac cactcgccat attgccgagt
     5401 ttcttgattt gtggattgaa ggagggaaaa acacgatggc aggatcaatg cgttctgtgt
     5461 tatctgatat gttccgcgag gccattgttg aaggacgtat atctcaaaat ccagtaacgc
     5521 caacaagagc accgaaaata gtagttacaa gagaacggct gaaactaaag acatacaact
     5581 gcatcaggga ggcagcagat caacttccga catggttccc attagctatg gacttagccc
     5641 ttgtaacagg acaacgtcgc gaagacataa caaatatgcg gttcagtgag atttatgatg
     5701 atcgtctcca catcaggcaa attaagacag gaatgatgat tgctatcccc ctgtcactca
     5761 gccttcctgt cgctggttta cgacttggta cagtagttga acagtgccgc atggtaagta
     5821 ggggggatta tctaatcagt gccgggatta gaaaaaacag ccctgacggc agccttcacc
     5881 cggacggcct gacaaagaaa tttgtcgcag ccagaaaatt aacaggtatc cagttcagtg
     5941 aaaacccacc aacttttcac gagatcagaa gcctggctgg acgattgtac aaagaaacat
     6001 gtggggaaga atttgctcaa cgtctacttg gccacacatc ggagaagaca acaaaaatgt
     6061 atcttgatga gagagaaaaa acgtacttac tgctctgatt ttaacgtaaa tggattgtta
     6121 aatgtgtttt ggttgtgata taaccaaaaa agaccg
//
LOCUS       DAZGOB010000202         6140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGOB010000202 DAZGOB010000000
VERSION     DAZGOB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.202"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..116)
                     /locus_tag="ACHLIF_004750"
     CDS             complement(<1..116)
                     /locus_tag="ACHLIF_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9608600.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLLEA"
     gene            complement(149..448)
                     /locus_tag="ACHLIF_004751"
     CDS             complement(149..448)
                     /locus_tag="ACHLIF_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO9608601.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSEQIQMEF"
     gene            complement(587..820)
                     /locus_tag="ACHLIF_004752"
     CDS             complement(587..820)
                     /locus_tag="ACHLIF_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9608602.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALANTSVGYLDQIVYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            934..1638
                     /locus_tag="ACHLIF_004753"
     CDS             934..1638
                     /locus_tag="ACHLIF_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9608603.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLNIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
     gene            1907..2269
                     /locus_tag="ACHLIF_004754"
     CDS             1907..2269
                     /locus_tag="ACHLIF_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608604.1"
                     /translation="MTQNVLPINKSLHDRAVDEFNRLHGTMIGEISAMLKTAKVAPLV
                     DLRKKDPTFSNVVAELRTFRDVCNALLPYFRVDKTSEIAVIDKLLILANDLAQAIDAD
                     DPDALCAAIAALDVEPYI"
     gene            2285..2413
                     /locus_tag="ACHLIF_004755"
     CDS             2285..2413
                     /locus_tag="ACHLIF_004755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608605.1"
                     /translation="MTKAFDYTAVSKLLAEMRGCVERVQNLRRDFEAHINHSQKAA"
     gene            2679..2898
                     /locus_tag="ACHLIF_004756"
                     /pseudo
     CDS             2679..2898
                     /locus_tag="ACHLIF_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2876..3148
                     /locus_tag="ACHLIF_004757"
     CDS             2876..3148
                     /locus_tag="ACHLIF_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO9608606.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(3172..3711)
                     /locus_tag="ACHLIF_004758"
     CDS             complement(3172..3711)
                     /locus_tag="ACHLIF_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HFO9608607.1"
                     /translation="MSNWWQELLRFFLRGLTLQQLIHMLIILIALIIITPASIKEWVD
                     IRNPEILPDHWMYYAMLLCISYVLNRGMEFIFLASSDRYKKYLSKRDEAKVIVETERL
                     FNSLSIQEKEVLAFAVMANNKIVLKHGDPVALSLMRKGLLHRSGVTYSASGKEKFVIP
                     DVWFHECYMRFAGKADELI"
     gene            3967..4935
                     /locus_tag="ACHLIF_004759"
                     /pseudo
     CDS             3967..4935
                     /locus_tag="ACHLIF_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
     gene            4960..5091
                     /locus_tag="ACHLIF_004760"
     CDS             4960..5091
                     /locus_tag="ACHLIF_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO9608608.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            5076..5228
                     /gene="kil"
                     /locus_tag="ACHLIF_004761"
     CDS             5076..5228
                     /gene="kil"
                     /locus_tag="ACHLIF_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO9608609.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PCYRRK"
     gene            5225..5383
                     /locus_tag="ACHLIF_004762"
     CDS             5225..5383
                     /locus_tag="ACHLIF_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608610.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTEAHVH
                     AESSLVSP"
     gene            5485..6090
                     /locus_tag="ACHLIF_004763"
     CDS             5485..6090
                     /locus_tag="ACHLIF_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO9608611.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
BASE COUNT         1948 a         1290 c         1395 g         1507 t
ORIGIN      
        1 gcctcaagca gcatatttga tagtctggcg taaccatcat cgagatctgc cacattacgc
       61 tcctgttcgg caaagttacc tctgccgaag ttgagtattt ttgctgtatt tgtcataatg
      121 actcctgtgg attgatccag taattccctc agaattccat ctggatttgt tcagaacgct
      181 cggtcttgca caccgggcgt tttttattgg tgagtccatc aagcgcatac ttaaaagctc
      241 tgctaatcgg actgatgtct gatgccattc cgaaagcaca caagaccgaa gcaataaatc
      301 tccagtccgt tctgcttatc ttcgattcat gacagccaat catctttgcc agaccgcgct
      361 gggtaagcgt tgacaggttg ataagtaaat ctgtttctgc gcgatcaaca tcacgctgtg
      421 atagtttgct gtaacttgtt tgttccattt cttaagattt ccaatagtga atagctagtt
      481 gaaaggtatg cgtggaaacg catatggcct tagttggtca gatatcttgg aactcgcttt
      541 tcagcgacgt aggacgaatg tccgttgtta caaagagcgg ggttacttat gctgctgatg
      601 ctctacgcga tacgaacacc agattttcct ttttcacagg tttataaccc gtgaaattac
      661 gagtggcttc ttcgattgca ttcgctttat ctggggaagc tcttcgaaac ccatatacaa
      721 tctgatcaag atagccaact gaagtattcg ctaatgcggc gagtcgcttc cattcctcac
      781 tagaagcctc ttttcgccag cgtagtagtt cattactcat tagtgcctcc gtttatcaca
      841 cagagcgact ttaccatttt gataaataaa acgcaatgaa gatttatcaa aatgcatatt
      901 tatccatttg ctaaatagag ggagaatcgt gggatggaaa acaaagatat tcgcaaatcg
      961 aatctggcgt ttttgctaga tgagcataaa aaaatcgcgg gtaacactaa tgcaagcttt
     1021 gccgataagc ttggagttag cccttctcaa ctcacgcaag tctccggtga aaaaagcact
     1081 cgaaacatag gggataaact agcaagaaaa ttcgaagccg cacttgggtt acctaatggg
     1141 tggcttgatt tggtacatga tgtaacacca attgcatcat gctcagattc tttaactttt
     1201 gtcggtcagg taagaaaagg gttagtgcgc gtggttggtg aggcaattct tggtgttgat
     1261 ggtgccatcg agatgaccga agagcgcgat gggtggctca atatttatag cgatgatcca
     1321 gatgcctttg gtcttcgtgt gaaaggagac agcatgtggc ccagaataaa atcaggagaa
     1381 tatgtactca ttgagcctaa caccaaagta ttcccggggg atgaggtgtt tgtcagaact
     1441 gttgaaggac acaacatgat caaagttctt ggctatgaca gagatggaga ataccaattt
     1501 acaagcatca accaggacca caggccaata acgttgcctt atcatcaagt agcaaaggtg
     1561 gagtatgtgg ctggtattct gaagcaatct cgccatctgg atgacatcga ggcaagggag
     1621 tggctgaaaa gttcgtgact tcatcgtcac atagctggta accagtggcc tgaagagacg
     1681 tttgggtgat gtgatagaga tcattattca attcgattga gaaaaatcat cataaggttt
     1741 atatccattt attagatttt atgtgtataa atagttaaaa atgcacgatg tagagatatt
     1801 tgtgcttaac atcaattaat cattgctaaa tcgtgcttat tgttcagaaa tcgcccaagt
     1861 catattgaaa atctgttggc gaacacctat ataaggagta tagttagtga cccaaaacgt
     1921 attgccaatc aacaagagct tgcatgaccg agctgttgat gaatttaatc gtctgcatgg
     1981 aacaatgatt ggcgagatta gtgcaatgct gaaaacagct aaagtagcgc cattggtgga
     2041 tcttcgtaag aaagatccta ccttttcgaa cgttgtcgca gaattacgga cattcagaga
     2101 cgtatgtaat gctctgctcc catactttcg tgtagataag accagcgaaa tcgctgtcat
     2161 cgataagtta cttatactgg ctaatgacct ggctcaagcc atcgatgctg acgatcctga
     2221 cgcattgtgc gcagcaatag cagcccttga tgttgagcct tacatttaaa cgaggggaaa
     2281 aaaaatgact aaagcgtttg actatactgc cgttagtaag cttttggctg aaatgcgcgg
     2341 atgtgtagag cgcgttcaaa atctacgccg cgactttgaa gctcacatca atcattcaca
     2401 aaaagcggct tgatacttaa gttagatgac agaacccggc ctcagcgccg ggttttcttt
     2461 gcctcacgat ccccacacct aataacacct taaccaattg tatttattga aaaattgata
     2521 gatacaactt tctaaaccac gaaattctga tccccctcaa gtctcttcat tccttctgat
     2581 aaattcccgc aatcaaataa aaaaccataa taatcaacag gatatttgaa aacccaaaca
     2641 atttatcatt ttgctattgc catcagttta tcatttcgat aaagtcaact catcagcagg
     2701 acgctgtaag ccaaacggag cagactggca ggctctttaa acaacgtcga acactcgact
     2761 acgtggctga aaagccagat cacccaacca cataagctgt gggatgcaat gccgaagtaa
     2821 ccgtctcagg aggagcttcg agattgcatc gccaaagttt attcgggagg aatctatgtc
     2881 cagaaaaaca gaatttaaag gcaccgcagc ttctcgccgt agagctcgtc gcgcaaacct
     2941 gcaaagtcag gaggcgatta gctccgacaa gctacacagg ccaacccctt cacgagtggt
     3001 cttgcaatgc aagcgcaaac cagcaatgag agcagaagtt ataactctga caacgttgac
     3061 cagaaaatat gaaggttcaa cttgtcttcc aaacgtagcc atttacgcgg caggctaccg
     3121 gaaatcaaaa caactgacag cgaggtgacg atcccccccg ccgtagagga actaaattag
     3181 ctcatcagct ttaccagcaa agcgcatata acactcatgg aaccagacgt caggtataac
     3241 aaatttctct ttacctgacg cgctgtaagt cacacctgag cgatggagaa ggccttttct
     3301 cataagagat aaagcgaccg gatcgccgtg cttaagtacg attttgttat ttgccataac
     3361 agcaaatgct aaaacctctt tttcctgaat actcagagaa ttgaataggc gctcagtctc
     3421 cacaattact ttagcctcat ctctcttact gagatatttt ttatatctgt ctgaagatgc
     3481 cagaaatata aactccatcc ccctgttcag aacataactg atacacaaaa gcattgcgta
     3541 atacatccag tgatctggaa gtatttcggg gttccttata tctacccact ctttaattga
     3601 tgctggggtg attattatca aggcaattaa aataataagc atatgaatta actgttgtag
     3661 cgtaagacca cgcaggaaga aacgtaatag ttcctgccac cagttactca tcggtgaatc
     3721 tccatcattc tctctgtagg ggtgaataga gtttatccga tttctcgctg taggggtaca
     3781 cgagaaccac cgagcctgat gtggttaaaa gacaggcata cgaataaaca ctgcactgtg
     3841 tattcattcc aacgagtgaa tacacggagc aatgtcgctc gtaactaaac aggagccgac
     3901 ttgttctgat tattggaaat cttctttgcc ctccagtgtg agggcaattt ttttgacgga
     3961 ggatatatga gtgaagtaac agatttagtt gttattgaaa aagcaaatgc aatgactgta
     4021 atttagtctg ccgaccagat tgaagaaatt cttcaaaagg ttgaacgtga agttatgtcc
     4081 tttgtgcctg atatcacaac ggcaaagggc agaaaggaga tcgcttatct ggcgtataaa
     4141 gttgcgcaga cgaaaacata tctcgatggt cttggcaaag accttgttgc tgaactgaag
     4201 gaaattccaa agctaattga tgctaaccgc aagacagtgc gcgatcgcct tgatgaactg
     4261 aaagccaagg cgcgccagcc tcttactgat tatgaggaag aacaggcgcg gattaaagcc
     4321 gaagaagaag ctaaggcagc agctgaagct ctcgcaaagc aaattgagtc tgaccatgaa
     4381 atagcgattt tgatggatcg cgaatttgac cgccaaagag aagaggcaag actcaaagcg
     4441 gagcaggaaa agcgagagca tgaagaacgc ttaaaaagag aagctgaaga gaaagccaga
     4501 gctgaagccg aagcaaaggc aaaagccgaa attgaagcag cagcaaggcg agaagcagaa
     4561 gctaaggccg cagaggaacg tgcagagcgt gaacgcattg aagccgagca acgagcacag
     4621 cgcgaagcaa aagaggcagc agaacgagct gaaagagaaa agcatgcggc aattgaagca
     4681 gaacgccgta aagcacagga ggaggcagaa cgaatcctgc gcgaggctga agcaaaagag
     4741 caagccagaa tagcagaaga aaaaagaatc aaggacgaag aagagcgtag agcaaaggat
     4801 aaagctcacc ggaaagaagt aaataacaaa atacttgctg accttatcaa ggttggcgca
     4861 tcagaagatg ttgctaaaaa tatcataaca gccatcgtaa aaggcgaatt attcgcaaca
     4921 aaaataacct actaataaaa ccaacataag gaaccaccca tgatttacgc aatcgcggga
     4981 ggcgctcgca tgggtgcctt ccaattaaat gaatctttac ttgaacgaat cacccgtaaa
     5041 ttacgtgacg gatggaaaag agttgaggtc ttattatgcg caatgaaata gccatcaatc
     5101 accagatgct tcgtgctgca cagaacaagg cagtaatagc ccgatttatt ggtgattcaa
     5161 aaatgtggct tgaagcaaat aaagcgatga aatcagctat caaccttccg tgttatcgca
     5221 ggaaatgagt tttacagata actggtcaga cgaagaattc attcgtcaga tgaaagaatt
     5281 aatcggtaac gaaggagata ttcatgtcac ttgcaaccac agtgaaggag agcaagttac
     5341 agaggcgcat gtacacgcag aaagctctct ggtatcgcca taatggtgac cgcgaaggaa
     5401 tgcgggtatg ccttaatttg tcccgagtcg aagtattaaa tcagcgttat ttccttgggc
     5461 cgtgtccatt ctgagaacaa tcatatgagc aaagaatttt acgcaagact ggcagctatt
     5521 caggagaatc tgaacgcgcc aaagaatcag tacaactcat tcggcaaata taaatacaga
     5581 agctgcgaag atattcttga aggcgttaag ccgttactga atggcctgtt tttatcaatc
     5641 agcgatgaag ttgtgttgat tggtgatcgg tattatgtga aagccacggc aactattacc
     5701 gatggcgaaa acagtcatac ggcaaccgct cttgcacgag aggaagaaag caagaaagga
     5761 atggattctg cacaagttac gggagctaca agctcttatg cacgcaagta ttgcctcaat
     5821 ggtttattcg gcattgatga tgcgaaagat gcagataccg acgagcataa acatcagcag
     5881 aacgcagcag caaagcaatc aaaaccatca cctacacctg aacaggttct aaaagcattc
     5941 actgacgcag caatgcagaa aaacaccgta gaagagctta aacaggcgtt cgccaaagcg
     6001 tggaagatgc tcgaaggcac accggagcag cacaaagcgc aggacgttta caacatcaga
     6061 cgagacgaat tagaaggagc tgctgcttaa tggcacattc gattactgta agactaaaca
     6121 agcccgcaag agagtttcag
//
LOCUS       DAZGOB010000203         5934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGOB010000203 DAZGOB010000000
VERSION     DAZGOB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.203"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            418..1434
                     /locus_tag="ACHLIF_004764"
     CDS             418..1434
                     /locus_tag="ACHLIF_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608612.1"
                     /translation="MSSVRTDKFIRYGFAVVAIVAILITTITTPNPTPYQYIVYRIVL
                     VLAAGCIGAVLPGFLEVKFNGWLRASGAVAIFAIVFFISPDLLQQNPESASKPVPPTV
                     IDAYAVNSSNRDRYLELFRTGQFGQRDKLRIGCVEWSESSCVAAGNFLRLLSEAGWEI
                     DSDAVFRMQPSVPVEGVSIVSRGDDLVGLPKVPPHMGRWSRSDISYEILTAAFKLMGV
                     PVYFSSDPTLQPGTTGVYFGPKPKLDAPVMQKDENVRRELLRYVGLGMTIQRACSQQP
                     VELCGGEIFSWETSVSSFLRQQRFNSDAVNRWLALPQVNAASATADMKIKLDLLMSFV
                     LGIE"
     gene            2078..4438
                     /locus_tag="ACHLIF_004765"
     CDS             2078..4438
                     /locus_tag="ACHLIF_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HFO9608613.1"
                     /translation="MKSMQSKTGSPLVRTEAELESRLTSALNIAFPNIPREDLIEQRH
                     FTVRLGHGTYKIDSAAHWKKYGRADVLIFHRERPLAVIELKREDLTLTHDDYEQAQSY
                     ANQLTPRPPLVVVTNGKDTRVYDSSNGQQWSGGQDASAAVNKMLANSAKLAAADMRWA
                     IEALMGRETNAWVPAVREETARLLIDITDQPGHSEHPFANNLLFPRKITSLVIESAVM
                     GTAFTIIEGDAQSGKSSCLREISLKTESSDLLAVLMLRGSGPGLFQALANLFAAEFEW
                     NLTSNDARNWLRRMSNCTEGPSLMLAIDDVEPGSQMATDLEELAGIRFGNRLVVVLTT
                     YHANALLKNPNGRTPSAIGSRSKVFKTSPMSLDEFKLAQQILSDQRIVFQQGAEYADD
                     YRSPWVLRTIYDDIVRNHQYQKTDLIAYLPPSPGMELIDAAQKSYESQYDLLRYYRVL
                     ARCALADTNSHSVELMFAKANGFVVRYDALSDEARGVVNELKHMGAVRIFRLSGWEDV
                     VVPTVPAAYLLELSDAVCDELVLRAEQDPQDAGAWLGERLDATYLGDMIGAQAIRRMA
                     AKERYFSFGIIQGLLSIEPYKEPIKNGLFTLAMPDNQQVNLKIEDGLAWISKHGDDAK
                     SVLVNLEDQIPKVISKSTSWMILGQLAKLPSAEVGDDDQRIDAYILLSIGRCPFPLIR
                     TNIEGLPYFEHNFGDQGDVLCLEKASIEVATQAMADLFSAPWLYADQWVDTAIATGSI
                     HLLHRLFAALNTVILRRIPVQSDWANEALNQRVSPALKEAIRSLSS"
     gene            4615..5754
                     /locus_tag="ACHLIF_004766"
                     /pseudo
     CDS             4615..5754
                     /locus_tag="ACHLIF_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5751..5906)
                     /locus_tag="ACHLIF_004767"
     CDS             complement(5751..5906)
                     /locus_tag="ACHLIF_004767"
                     /inference="COORDINATES: protein motif:HMM:NF015301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PapB/FocB family fimbrial expression
                     transcriptional regulator"
                     /protein_id="HFO9608614.1"
                     /translation="MTKGNISERDFDILIKLSGIHSEKAILALKKYYVDGVIRKIDPT
                     HVMWTRP"
BASE COUNT         1527 a         1326 c         1474 g         1607 t
ORIGIN      
        1 cttacttggc tttagtcccg gagtgttgcc aggagtatct ttattatctc gtcggcgttg
       61 atctggtttt cctgtcgatt tagcaattgg ttttggacca ggtttaagac ccatgatagt
      121 tatccttttg agatgtaaga gggaagccct caacattggt atattggata ctgagaaatt
      181 tttcaatggg agtgataaac ggccgaatga ttaactcgat attgtgatca acgtctgttt
      241 tttgcacact gtagactgac tcttggagca gaaggttgct ctgagcgagg aatggacagt
      301 tacactcatc accaacgcca atagcctcat tacgcgtata aagctgtatt aatcattggg
      361 gagcagttta atgcgggatt ttcgacttat ggacttgatt caggtgtcaa aattaaaatg
      421 agcagtgtaa ggacagacaa gttcattagg tatggttttg cggttgtggc gatagttgcc
      481 atactcatta ctactatcac aacccccaat cccacacctt atcaatatat agtttaccga
      541 atagttctgg ttctggctgc cggatgtatt ggtgcagttc tgcctggttt ccttgaagta
      601 aaatttaatg ggtggttaag agcatctggc gcagtggcga tcttcgctat agtgtttttc
      661 ataagcccag atttgcttca acaaaatcct gaatctgcaa gtaaacctgt accgcctact
      721 gtgattgatg catatgccgt taatagttcc aatcgcgaca gatacctcga attatttcga
      781 acaggacagt tcggtcagcg ggacaagctg cgcatcggtt gtgttgagtg gagtgaatca
      841 tcgtgcgtgg ctgctggtaa ttttttgcga ttgttaagcg aagctggctg ggaaatcgat
      901 tctgacgctg tattcagaat gcaaccatcc gtgccagttg agggggtatc gattgtaagc
      961 cgtggcgatg atctggtcgg tttgccaaaa gtaccacccc acatgggccg ttggagtcga
     1021 tcagacatta gttatgaaat tttgacagcg gcatttaaat tgatgggagt tccggtttat
     1081 ttttccagtg acccgacatt acaaccaggt actaccggag tgtactttgg accgaaaccg
     1141 aaactggatg caccagtcat gcaaaaagat gagaatgtgc gacgtgagct tctccgctac
     1201 gtcggtttgg gaatgactat tcagcgagcc tgctctcagc agcccgtcga actttgtggg
     1261 ggtgaaatct tttcctggga aacgtcggta tcttcgttcc taaggcagca gagattcaac
     1321 tccgatgccg taaacagatg gcttgccttg ccgcaggtta atgcggcttc ggcaactgca
     1381 gacatgaaaa taaagctaga tttgttaatg tcatttgttc tgggcatcga gtaactatac
     1441 ctcttgcgag gatgattgag cgtctaatgg accagcttag ccgttagcgc tatttgttta
     1501 cgtaagcctg tggaatccaa ctagtgcttc tacgatgcgt tgtttggtga aaaaaaagtg
     1561 ttgaagcact ggttttagac aactaaagcc acgatttgct catgcgtcga aaatgtagtc
     1621 gtctactgtg tagcacggag gcgatattcg tacgcttaca cttgccgata ggccactggg
     1681 gagtgttctt ggcactaagc tgccaggccc gaaggtctgc tatgagcgag gggctgacat
     1741 tcctattggc attcggtgtg agttatcggg ggcggtcatg cggcatactt ccgatacagg
     1801 cgtttccagc tctgcctgtt tcagggttat attaatctgc tttagaatta atcgtggctt
     1861 tagcatctgc acatcgtgct ggagaaacct gagtgaacta aatcatattc cctttcttca
     1921 gctaatcaat tgatagttat gcggaaaaag ttactttgct tgtcaataat ccatttcaca
     1981 tccgttaatc caatgctatg cgtcttcgca ttaatctgga aaggtagtaa gtgaaataaa
     2041 aacttagcca atgactaata tctgaggata ctttgtcatg aaaagtatgc aatcaaaaac
     2101 gggctctcca ctcgttcgaa ctgaagccga gctagaaagc cggcttactt cagcactaaa
     2161 catagcattt cctaacattc ctcgcgaaga tcttatcgaa cagcgccatt ttaccgttcg
     2221 gctaggccat ggaacttaca aaatagatag cgccgcacac tggaaaaaat acgggcgagc
     2281 ggatgttttg atctttcatc gggagagacc acttgcggtt attgaactga aacgtgaaga
     2341 cctgacactg acccatgatg attatgaaca ggctcaaagc tacgctaacc agttaacacc
     2401 acgtccgcct ctcgttgtag ttacgaacgg taaagatacg cgcgtttatg actcaagtaa
     2461 tggacaacag tggtcgggtg gacaggatgc ctcggcagcg gtaaacaaga tgctcgccaa
     2521 ttctgcaaag ttggcggcgg ctgacatgcg ctgggctatt gaagccttga tggggcgaga
     2581 aactaatgct tgggtgccag ctgtgcgaga agaaacagcc cggctgctga ttgatataac
     2641 tgatcagcct gggcattcag aacatccatt tgccaacaac ctactttttc ctcgcaaaat
     2701 cacgtctctt gtaattgaat ctgctgtgat gggtacagct ttcactatca ttgagggtga
     2761 tgcacaaagc ggaaaaagca gttgcctccg tgaaatttcg ctaaaaacgg aaagtagcga
     2821 tcttttggct gtcctgatgt tacgaggttc cggaccagga ctatttcagg ctctggccaa
     2881 tctttttgca gctgaatttg aatggaattt aacctcaaat gatgcacgta actggttaag
     2941 acgaatgtct aactgcactg aaggtccatc gcttatgctt gctatcgacg acgttgaacc
     3001 aggctcccaa atggctactg atcttgagga acttgcgggt atacgttttg gcaacaggct
     3061 ggtggttgtt ctgacgacct atcatgccaa cgcgttactg aaaaatccaa acggacgaac
     3121 tccgtctgca attggtagcc ggtcgaaggt atttaaaaca tcgcctatgt ccctagatga
     3181 atttaagctg gcgcaacaga ttctctcgga ccagcggata gtttttcaac agggagcaga
     3241 atacgccgat gactaccgct cgccttgggt tcttcgcact atttatgatg acattgtgcg
     3301 caatcatcaa taccagaaaa cggatcttat cgcctatctt cctccatcac ccggtatgga
     3361 acttatagat gctgcgcaaa agagctatga gagtcagtat gatcttcttc gttattatcg
     3421 ggttcttgca cgctgcgcac tagctgacac aaactcccac tcagtagaat taatgtttgc
     3481 taaagcgaat ggatttgtcg tgcggtatga cgcactttct gacgaagctc gtggtgtagt
     3541 gaatgagtta aaacatatgg gggcagttcg tatttttcgc ctttcaggat gggaagatgt
     3601 tgtggtgccg acagttcccg cagcttacct tctggaactg tcggatgctg tttgtgatga
     3661 actggtttta cgggcagaac aagaccctca agacgccggg gcttggcttg gcgaacggct
     3721 agacgccact tatcttggag atatgatcgg tgctcaagcc atacgccgta tggcagctaa
     3781 agagagatat ttcagctttg gaattattca gggccttctc agcattgagc catataaaga
     3841 acctataaaa aacggattgt ttactttagc catgccagac aaccaacaag tcaatctaaa
     3901 gattgaggat ggattagcgt ggatttctaa acacggggat gatgcaaaga gtgttctggt
     3961 gaatttagag gatcaaatcc caaaggtcat ttctaaatca accagttgga tgatactcgg
     4021 gcaactagcg aaactgccgt cagcagaggt gggtgacgat gatcagcgta tagacgccta
     4081 tattttactg agcattgggc gctgcccatt tcctctaatt cgcacgaata tagaaggatt
     4141 gccttacttt gagcataatt ttggcgatca gggtgatgtt ctttgcctag aaaaagcgtc
     4201 tattgaagtt gctactcagg cgatggctga tttattttca gccccatggc tctatgcgga
     4261 ccagtgggtg gatacagcta tcgcgaccgg ttctatacat ttactgcatc gtttgtttgc
     4321 tgctttaaac acagtgatat tgcgtagaat acccgtgcaa tccgactggg ctaatgaagc
     4381 acttaatcaa cgtgtctcac cagcccttaa ggaagccata cgatcattat cttcgtgact
     4441 aagcaatggg attttaatac ataacgagca tctgtccacg caagttaaca attgccttgg
     4501 ctcgatattt tggtttctag tgcatatatg ctgatcttct cttcgttgat ctgatcttac
     4561 ccaccaatag tggacacagg actaagtgag taaactctca accagaggtg actcatgaca
     4621 aaaccagtat caatcagcaa gaagccccgt gaacaacatt cgcctgaatt tcgtaacgaa
     4681 gccctgaaac tcgctgaacg catcggtgtg gccgccgcag cccgtgaact cagcctttat
     4741 gaatctcagc tttatgcctg gcgcagtaaa cagcagcaac aaatgagttc gtcagagcgc
     4801 gaaagcgaac tggccgctga aaatgtccgc cttaaacgac aactggcgga gcaggctgag
     4861 gaactggcca tcctccaaaa ggccgcgaca tacttcgcga atcgcctgaa atgaagtatg
     4921 tcttcatcga aaatcatcgg gcagagttct gcatcaaagc gatgtgtcgt gtacttcggg
     4981 ttgcccgcag cggctggtat gtctggctca ggcgtcgtca ccagatgagc cttcgccaac
     5041 agtttcggct cacctgcgat gccgctgtcc ataaggcatt ctttgaggca aagcagcgat
     5101 acggtgctcc ccgccttgct gacgaactgc cggagttcaa tattaaaacc attgccgcca
     5161 gcctgcgtcg tcaggggctg cgggcgaaag ttcagcccgg tcagctaccg tgcacatggc
     5221 ctgcccgtat tggagaatct gctggagcag gacttcagcg ccagaggccc gaaccagaag
     5281 tgggcgggtg acatcacgta cttgcgtacc gatgagggct ggttgtatct cgcagtagtc
     5341 atcgacctgt ggtcacgcgc cgtcattggc tggtcgatgt caccgcgaat gacagcacaa
     5401 ctggcctgtg atgcactaca aatggcgttg tggcggagaa gacgcccgga aagcgtcatt
     5461 gttcatacgg accgtggtgg tcaatactgt tcaggggatt atcaggcgct gctgaagcga
     5521 cacaacctgc gtggcagtat gagtgcgaaa ggctactgtt atgacaatgc ctgtgtggaa
     5581 agcttctttc attcgctgaa ggtggaatgt atccacgggg aacgctttag cagccgggaa
     5641 ataatgcggg caacggtgtt taattatatc gagtgtgatt acaatcgctg gcgtcgtcac
     5701 agtgcctgtg gcggtctcag cccggaacaa tttgaaaacc ataatctcgc ttagggccgt
     5761 gtccacatta cgtgggtagg atcaattttc ctgatgaccc catcgacata gtattttttc
     5821 aaagcaagaa ttgccttctc gctatgtatt cctgatagct tgatcaggat atcaaagtct
     5881 ctttcagata tattcccctt tgtcatgtgt cactccatga atcaaatttc atag
//
LOCUS       DAZGOB010000204         5877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGOB010000204 DAZGOB010000000
VERSION     DAZGOB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.204"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..207)
                     /locus_tag="ACHLIF_004768"
     CDS             complement(<1..207)
                     /locus_tag="ACHLIF_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFO9608615.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGK"
     gene            511..1443
                     /gene="rfaD"
                     /locus_tag="ACHLIF_004769"
     CDS             511..1443
                     /gene="rfaD"
                     /locus_tag="ACHLIF_004769"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO9608616.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1453..2499
                     /gene="rfaF"
                     /locus_tag="ACHLIF_004770"
     CDS             1453..2499
                     /gene="rfaF"
                     /locus_tag="ACHLIF_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO9608617.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2503..3495
                     /gene="rfaC"
                     /locus_tag="ACHLIF_004771"
     CDS             2503..3495
                     /gene="rfaC"
                     /locus_tag="ACHLIF_004771"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO9608618.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3492..4700
                     /gene="rfaL"
                     /locus_tag="ACHLIF_004772"
     CDS             3492..4700
                     /gene="rfaL"
                     /locus_tag="ACHLIF_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO9608619.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNSVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4736..>5877)
                     /locus_tag="ACHLIF_004773"
     CDS             complement(4736..>5877)
                     /locus_tag="ACHLIF_004773"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO9608620.1"
                     /translation="VDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKN
                     PGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVI
                     VVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANA
                     DIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLA
                     AAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLA
                     DLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSIS
                     SDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         1645 a         1286 c         1304 g         1642 t
ORIGIN      
        1 ttttccgttg cgaatcgctt caacccttgg tttcaaatcg ggagaagtga aaataatcat
       61 ctcattttca agtgcggcca gacgctcaaa gtaactaaaa taaacatcaa ctgaacgagc
      121 tagtttttca cggaaccctt tattagcagt ccaatcccct ctaccaatgt caaaatatgc
      181 agtgataatt gtggtggatg atttcattac taataagacc attaatataa aaaaagaatt
      241 atatcataaa tattaacaga atgtatactg tatattatcc attcaaatgc ttccgttatt
      301 tatcatacat cggaatattg atactaaagc actattcaca tgcaaaacca acatccgcca
      361 tgaaggacta gctaaaaccc aaactagttt gttgcaatta gcatccttgc acctctatgt
      421 aaagggctga agggattcgg atgtgatggt atgattacag acattcgtgt ctgagattgt
      481 ctctgactcc ataattcgaa ggttacagtt atgatcatcg ttaccggcgg cgcgggcttt
      541 atcggcagca acatcgttaa agccctgaat gataaaggca tcaccgatat tctggtggtg
      601 gacaacctga aagacggcac caagtttgtg aacctggtgg atctgaatat cgcagactat
      661 atggataagg aagacttcct gatccagatt atggctggcg aagagttcgg cgatgtcgaa
      721 gcgattttcc acgaaggcgc gtgctcttcc accaccgagt gggacggcaa gtatatgatg
      781 gataacaact atcaatactc caaggagctg ctgcactact gcctggagcg tgaaatcccg
      841 ttcctgtacg cttcttccgc agccacctac ggcggacgca cctccgactt tattgaatcc
      901 cgcgagtacg aaaaaccgtt gaacgtctac ggttactcaa aattcctgtt tgatgaatat
      961 gttcgccaaa tcctgccgga agcgaactcg cagattgttg gcttccgcta tttcaacgtt
     1021 tatggaccgc gtgaaggcca taaaggcagc atggcgagcg tcgctttcca tctcaacacc
     1081 cagcttaata acggtgaatc accgaagctg ttcgaaggta gcgagaactt taaacgcgac
     1141 ttcgtctatg ttggcgatgt ggcagatgta aacctgtggt tcctggaaaa tggcgtttcc
     1201 ggcatcttca acctcggcac cggtcgtgcg gaatccttcc aggctgtagc tgatgctacg
     1261 ctggcttatc acaagaaagg ccagatcgaa tacattccgt tcccggataa gctgaaaggc
     1321 cgctaccagg cgttcactca ggcagatctg acaaatctgc gcgcggcggg ttacgacaaa
     1381 ccgttcaaaa ccgttgctga aggtgtaacg gaatacatgg cctggctgaa tcgcgacgca
     1441 taagagctct gcatgaaaat actggtgatc ggcccgtctt gggttggcga catgatgatg
     1501 tcgcaaagtc tctatcgcac gctccaggcg cgctatcccc aggcgataat cgatgtgatg
     1561 gcaccggcat ggtgccgtcc attattatcg cggatgccgg aagttaacga agctatccct
     1621 atgcctctcg gtcacggagc gctggaaatc ggcgaacgcc gcaaactggg tcatagcctg
     1681 cgtgaaaagc gctacgaccg cgcctacgtc ttacctaact ccttcaaatc cgcattagtg
     1741 cctttcttcg cgggtattcc tcatcgcacc ggctggcgcg gcgagatgcg ctacggttta
     1801 ctcaacgatg tacgcgtgct cgataaagaa gcctggccgc taatggtgga acgctatata
     1861 gcgctggcct atgacaaagg cattatgcgc acagcacaag atctgccgca gccattgtta
     1921 tggccgcagt tgcaggtgag cgaaggtgaa aaatcatata cctgtaatca attttcgctt
     1981 tcatcagaac gtccgatgat tggtttttgc ccgggtgcgg agtttggtcc ggcaaaacgc
     2041 tggccacact accactatgc ggagctggca aagcagctga ttgatgaagg ttatcaggtg
     2101 gttctgtttg gctcggcgaa agatcatgaa gcaggcaatg agattcttgc cgctttaaat
     2161 accgagcagc aggcatggtg tcggaacctg gcgggggaaa cacagcttga tcaagcggtt
     2221 atcctgattg cagcctgtaa agccattgtc actaacgatt ctggcctgat gcacgttgcg
     2281 gcggcgctca atcgtccgct ggttgccctg tatggtccaa gtagcccgga cttcacaccg
     2341 ccgctatccc ataaagcgcg cgtgatccgt ctgattaccg gctatcacaa agtgcgtaaa
     2401 ggtgacgctg cggagggtta tcaccagagc ttgatcgaca ttactcccca gcgcgtactg
     2461 gaagaactca acgcgctatt gttacaagag gaagcctgac ggatgcgggt tttgatcgtt
     2521 aaaacatcgt cgatgggcga tgttctccat acgttgcccg cactcactga tgcccagcag
     2581 gcaatcccag ggattaagtt tgactgggtg gtggaagaag ggttcgcaca gattccttcc
     2641 tggcacgctg ccgttgagcg agttattcct gtggcaatac gtcgctggcg taaagcctgg
     2701 ttctcggccc ccataaaagc tgaacgcaaa gcgtttcgtg aagcgctaca agcagagaac
     2761 tatgacgcag ttatcgacgc tcaggggctg gtaaaaagcg cggcactggt gacacgtctg
     2821 gcgcatggcg taaagcatgg attggactgg caaaccgctc gcgaaccttt agccagcctg
     2881 ttttacaatt gtaagcatca tattgcaaaa cagcagcacg ccgtagaacg cacccgcgaa
     2941 ctgtttgcca aaagtttggg ctatagcaaa ccgcaaaccc agggcgatta tgctatcgca
     3001 cagcattttc tgacgaacct gcctacagat gctggcgaat atgccgtatt tcttcatgcg
     3061 acgacccgtg atgataaaca ctggccggaa gaacactggc gagaattgat tggtttactg
     3121 gctgattcag gaatacggat taaacttccg tggggcgcgc cgcatgagga agaacgggcg
     3181 aaacgactgg cggaaggatt tgcttatgtt gaagtattgc cgaagatgag tctggaaggc
     3241 gttgcccgcg tgctggccgg ggctaaattt gtagtgtcgg tggatacggg gttaagccat
     3301 ttaacggcgg cactggatag acccaatatc acggtttatg gaccaaccga tccgggatta
     3361 attggtgggt atgggaagaa tcaagtagag tgtcgttcta caagtatgtc tcttgcagat
     3421 ttgccagctc aaaccgtttt tcaaaattta aatcttgaaa taataaccaa taagttgaca
     3481 tcggagataa gatgacctca acattatttt tctctctcga gaaaaaaaac tggatagcgt
     3541 actggaacag agctctcgta ttcttattca ttaccaccta ttttttgggt gggattacaa
     3601 ggtataaaca tcttattgtt attcttatga caataacgac aatcgtctat ctctgcaaac
     3661 ggccaaaaca ctatctctca ctatttaaaa catttctttt tggtagtgtt gccatattaa
     3721 ctattgctgc attgctgtca cttcttcaat cccctgatgc aggtgctagc atgaaggaaa
     3781 ttttcaaagc tattattgag aatactttac tatgcacaat agcaataccg atcatattga
     3841 gagacgagaa aagagaagat gtcgaaaaaa tcgttttctt ctcatttatt agtgcgttgg
     3901 gcttacgctg tttttctgaa ttgattacct attataagga ctatcaacaa gggataatgc
     3961 cattcgcaga ttatagacac cgtagcattt ctgactcgat ggtcttttta ttccctgcat
     4021 tgttaaatct ctggcttatc aaatcagcaa aataccgcat ttcttttgtg gttctaagcg
     4081 ttatttttat ttttctgata ttaggaactt tatccagagg ggcctggctt tccgtgttag
     4141 tcattggatt aatatggatt ctgatgttta aacaatggaa gttactatta gtaggagtaa
     4201 tggttgccat cattgcattg tcggttattt tcacacataa ggagatgacc gcaaagctaa
     4261 cgtataaact tcaacaaact aatagttctt atcgctatgc aaatggtact caaggcagcg
     4321 cactcgatct aatattagaa aatcctgtta ttggttatgg ttacggtaac gttgcatata
     4381 aagatgtcta taataaacgt gtcattgatt atccagaatg gacctttaga caatcaatag
     4441 ggccacataa ttttgcgcta ttcatctggt ttggcactgg tttattaggg ctggtaagtc
     4501 ttatgatgct atactgtgca atattgaaag agtgtataaa aaatagcgtc aagaataaat
     4561 atcgctcacc atataatgca tattatataa tcttactatc ttttataggt tattttgtta
     4621 tccgtggaaa cgtagaacaa attgaaccaa atttattagg cgtttacgcc ggcttattat
     4681 tagcgatgaa aaacaagtaa aaaataaaaa aggctgcata atgcggcctt tttatttatt
     4741 caaaccaatt atgaataacc tcttcgaatc tttgagttac gccatcccaa ctgtagtgtt
     4801 caaacacaaa gtcctgacct tgctttgcaa ctgctgttaa ttcaggattc gctaacgttt
     4861 ttaaaatatc gctgcttatg gagtctgcag tcataggttc ttttaaatgg aatcccgtag
     4921 tattttcttt gacaaattcc gtcatccccc cacgcgtact cactaaaaca ggcttaccag
     4981 cacccatagc ttcaattgcc accatgcaaa atggttcctg gaactgggaa ggaatgacga
     5041 ctaaatctgc cagtgggtaa taacaatgca tcttttcagg cggtatgctt cctaacataa
     5101 tacaacgatc cttcaaacgt tttgcaattt ccctgacatt gcgttgatat gcacctttgt
     5161 cacttttact catttctgta taatcaccaa tcacaactaa ttttaaattt ttgtgagcag
     5221 cggccaattt ttcgaatgat tgcatcaaca gcaatatgcc tttgtcaggg acgatacgcc
     5281 cagcataaaa aataattttc tcttcagggg agatattaag ttcgcttcta gtgatcggct
     5341 gaaaattaga ttgataggtt tctaaatcaa ttccatttgg tacaatctca atatctgcat
     5401 ttgctaaata agattggtaa tactttttca agtataaact aggcactatc attttaacat
     5461 tttgctcaag caattttggt tcaaatgcat tatgcatatg tataaccact cttgcctgag
     5521 gagctcgctt acgaatttga gtgtatagct tcatgctatt atgaactaca ataacacttt
     5581 ctttcgttat aggaaaatca tgtgcaatat tcaatattct ctgtgaatat ggaagaggat
     5641 caagtcttgt ccatttttga aataaccgct tatatatgcg actgaatcca accctatgaa
     5701 tactacaatt atcattaaca aaggtgtaat ttgagtatcc tggatttttg atacaaacaa
     5761 tacgatttgg aaaattggta cgctgagcaa cctgatacat ccacgtttca acagcagccg
     5821 ctccgcgagg aggaattgaa aaaataggag taaccgtaaa tattatttta tcaacca
//
LOCUS       DAZGOB010000205         5839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGOB010000205 DAZGOB010000000
VERSION     DAZGOB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.205"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..288
                     /locus_tag="ACHLIF_004774"
                     /pseudo
     CDS             49..288
                     /locus_tag="ACHLIF_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHLIF_004775"
     CDS             complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHLIF_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO9608621.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(965..1639)
                     /locus_tag="ACHLIF_004776"
     CDS             complement(965..1639)
                     /locus_tag="ACHLIF_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO9608622.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHLIF_004777"
     CDS             complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHLIF_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO9608623.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHLIF_004778"
     CDS             complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHLIF_004778"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO9608624.1"
                     /translation="MFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGM
                     GQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIG
                     VSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCT
                     PGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVI
                     NCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITA
                     CAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAE
                     DVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRY
                     IAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDI
                     NGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRT
                     VQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFLF"
     gene            complement(3810..4130)
                     /locus_tag="ACHLIF_004779"
     CDS             complement(3810..4130)
                     /locus_tag="ACHLIF_004779"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO9608625.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4139..4441)
                     /locus_tag="ACHLIF_004780"
     CDS             complement(4139..4441)
                     /locus_tag="ACHLIF_004780"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO9608626.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(4451..5230)
                     /locus_tag="ACHLIF_004781"
     CDS             complement(4451..5230)
                     /locus_tag="ACHLIF_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO9608627.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            5611..>5839
                     /locus_tag="ACHLIF_004782"
     CDS             5611..>5839
                     /locus_tag="ACHLIF_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608628.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGP"
BASE COUNT         1305 a         1533 c         1578 g         1423 t
ORIGIN      
        1 ctgtagattc aatctgtcaa tgcaacaccc ctttcaatta tctctttcac tgcccgcgac
       61 ggtatcggtt atcagtttga cctgctggct aacggtgaaa ccgtactgcg tgatgcatcc
      121 ggcgaaaaac gcatcaaggg tgaaaaagac ggtcgtatgc tcatgaatgt tggcctgaat
      181 gcggaaattc gcgacaacat ccgcttcggt ctggagtttg aaaaatcggc atctggtaag
      241 tacaacgtgg ataacgcggt gaacgccaac ttccgttatt ccttctgata aaaacaaggg
      301 gccgcctgcc ggccctgata cagaggatgt tgtgattact tacccagcat gccctgctct
      361 tcgataaagg cgatgatgtt ttgcagacct tcaccacttt tcaggttagt gaacccccat
      421 gggcgttctc cgcgcatacg taaagtatca cgctccatta cctcgaggga ggcgcccacg
      481 tatggtgcaa ggtcagtttt attgatcacg aggaagtcgg atttggtgat ccccggaccg
      541 cctttacgcg ggatcttttc tccttctgcc acatctatga catatatggt aaggtccgcc
      601 agttcggggc tgaaggtcgc actcaggttg tcgccgccgc tttccacaaa gatcagatcg
      661 agattaccaa atttctcact cagtgcttcc acggctgcca gattcattga tgcgtcttca
      721 cgaatggctg tatgagggca gccccccgtt tcgacaccga caatgcgttc cgcttccagt
      781 gctccggcct cagtgagaat gcgttgatct tctctggtgt agatgtcgtt ggtgactacg
      841 gctaattgcc aggtgtcacg catggatttg cagagggctt ccagaagcgc ggttttacct
      901 gagccgaccg gaccgccgac gccgacacgc agcggttgct tgattatgtt cattttattt
      961 tctcctaaga acggaataat cgggaatact gggtttcatg tcgcgcggaa gcgatagatg
     1021 caagtggcgt tgctgaacca attgcgtgat ccggggttgc aagggcactg tccatgttcg
     1081 ccgcatagcg ggcacacagg cgcataatta actgctgggc agccagctgg ccgtagggta
     1141 ccagcctgac gcccgccatc actgcgcttt caatccagct gtagccaagg ctcagggcca
     1201 ggtctgaaac ggatatttgc cagcgtacgc cgagccatgc cataccggtt aactggcttt
     1261 gctggcatag tttgcgccat tcggccgggc agtcgggctg ccagtcgacc agaagtctgg
     1321 tgaaggctgc accgcggtta cgttcttcat cgcgtagctc acgggtttcc ctgcaggcca
     1381 gcagataagc ggtccagcgc ccggcactgt caggatcgcc cgcttcacag gcccggtata
     1441 gccgggcaag cagtggcagg tcgacggcaa aaaaactctg ttccatctgc aactgctgcc
     1501 agtgttcaaa ggcggcgcta tcttctaccc agcctgcttc cactgcccac tccagtccct
     1561 gggaccagct gtagcctcca acagggaggc tgttactggc aagctgcatc agccgcagac
     1621 gtttttccgg agtcggcatt cagtggctgt gctcctgatc gtgattatgg gcgtgcgact
     1681 tgctggcgta agcgccagct tccggctcaa acggaagatg tgcaaaatca acatccagcc
     1741 caaactggcg cagcatatca tcgaggacgt gatcgtgatg gtagcgcagc tctccgggca
     1801 ttatttgcag cggaacgtgg cgattgccga gatgccagca ggctttcgcg agcataaaag
     1861 gatctgcgca gcgcactaca gaaacggctt catcagcggc gatcacctta atgaattcat
     1921 caccgttttc gttactgaga atatcaccat cgcgcagcag cagaccgcgt ggcagcaaca
     1981 gtccagcctg acgtccatcg ttaagggtga ctttaatacg gctttttacc cgcatgtcaa
     2041 cagggagggt aacgctggca gttgtctgag ccggggtctc cacccgccgg gtcaaataaa
     2101 gcatttgtgc tccttaaaac aggaaataac gttgcgccat tggcagaatg tccgccggtt
     2161 cactggttat cagttcgccg ttgatacgca cttcgtaggt ttgtgaatcc acggttatat
     2221 caggcaacag gctgttgtgg cgcatatcgg ctttttgtac tgtgcggcag ccttttacca
     2281 cagcagttgt gctgtgcagg ttaagctgtt cagcgacgcc atttgctgct gctgcctgcg
     2341 acaggaaagt cacacgacag cggtgacggg cactgcccaa tgcagcgaac attgggcgat
     2401 agtgcaccgg ctgcggtgtg gggatagagc cgttgatatc acccatcggc gccatggcta
     2461 tcattccgcc tttgacgata gtcgccggtt ttacgccaaa gaacgccggg gaccagagca
     2521 ccagatccgc cagttttccc acttcaatcg agccgacttc atgagcaata ccgtgggtta
     2581 atgccggatt aatggtgtat ttagcgatat agcgcttcac gcggacgttg tcgttatcac
     2641 cactttcttc cggtaacggg ccgcgctgaa ctttcatccg gtgtgccacc tgccaggttc
     2701 gtaacactac ttctccgacg cgtcccatgg cctgcgaatc ggacgaggtg agggagaacg
     2761 cgccaaggtc gtgcaggacg tcttccgcgg caatggtttc ctggcgaatg cgcgattcgg
     2821 caaaggctac gtcctcggcg atatccgggt caagatgatg gcaaaccatc agcatgtcca
     2881 gatgctcatc aatagtgttg acggtatagg gtagcgtcgg attggttgag gaaggcagaa
     2941 tattggggtg cgcgcaggcg gtgataatat ccggagcatg accaccacct gcaccttctg
     3001 tatggaaggt atggatagtg cgcccgccga tggcagtcag agtatcctca acgaatcctg
     3061 attcgttaag cgtatcactg tgtagcgcaa cctgaacgtc catttcgtcg gctacagtca
     3121 gtgcgcagtt gattaccgca ggtgtagctc cccagtcttc gtgaattttg aggccgataa
     3181 ccccggccgc gacctgctca cgaagcgcat ccggattgga gcaattgcct ttacccagca
     3241 acccgatatt gaccggcagg ctgtctgcag cctgcagcat ttgataaatg taccatggtc
     3301 ctggggtaca ggttgtggcg ttagaacccg ctgtcgggcc agtaccgcca ccgatcatgg
     3361 tggtaatgcc ggatgtcagc gcttcttcag cctgctgtgg gcagatccag tgaatatgcg
     3421 tatcgacgcc acctgcggta acgatcctgc cttctgcggc aataatttcc gtggatacgc
     3481 cgattgggat agtgacgttg ggttgtatat caggattacc ggctttgccg atagcaaata
     3541 tccttccatc tttgacgccg atatcggctt taacgatccc ccagtaatcg atgatcaggg
     3601 cattggtcag caccagatca gcgcagccgg cggagagcat ttgcccctgt cccataccgt
     3661 cgcggattac tttaccgccg ccgaatttga cctcttcgcc gtaggtagtt aaatcatctt
     3721 cgacctcgat ccacagctca gtgtctgcca gacgaatttt atcaccggtg gtagggccga
     3781 acatgtcagc ataggcctag cgtgaaatat tactcatcat ttgcctccag ctcacccatt
     3841 atatctccgc ggaaaccgtg cacgatacgg gcaccgctca gcgccaccag atcaacctca
     3901 cgcttttggc ctggttcgaa gcgcactgca gttccggcgg cgatattgag acgatagcct
     3961 cttgccttct ggcgatcgaa cttcagtgcc gggttaacct ccgcgaagtg ataatgggaa
     4021 ccgacctgaa ttggtcggtc tccgtgattt tcaacgataa tactgcaggt agcccggccg
     4081 atgttaagct ctatatagcc aggtttaacc ttatattctc cagggatcat ctgttacctc
     4141 agattatcgg attatggacg gtaaccagct tggagccgtc cggaaaggtg gcttcgacct
     4201 ggatatcggg gatcatttct ggtatgcctt ccatgacctg ctcgcgactc aggacatgcc
     4261 gtccttcttc catcagcgca gcgacgcttt tgccgtcgcg agcgccctcc attataaaag
     4321 cgctaatcag ggccacggat tcgggatagt taagtttcag gccgcgggcc agacgacgct
     4381 ctgccagcag tgcagcggta aacagtaata gtttgtcttt ttctcttgga gtcagttcca
     4441 tagcgttctc ttaagtcagc cagatccggg gaagtaccgg tgatttgtca gtcagttgcg
     4501 gccgcataaa ttgccagata tcacgcatta cctgctggca acgcagatta tcatcactta
     4561 aaaagcgaac tgtcagtaga ccatcggtga gcgtggctcc ggcgtagttt tccagtggtg
     4621 tgagtttttc gcgtatgtct tccagttgcg tttcattccc caggtagaac agcatcgttc
     4681 ccacccatgg tttccgggca acgcaggtaa gttctccatt ggccacgtgc agacgctcaa
     4741 tcaacagcgg agaaccgtcg acccatatct ccagacggtt agcgagtgtt ccgtggctga
     4801 aggtttctcc gatgaccgga cgtccaaggc acagaagatc ccaggccagc agtgtactgg
     4861 aggacgacag atgaaacacg gtatgcaacg tggcgtgagc gccgggaaaa aaaatcgaat
     4921 cctgtggcaa ccactccagc gttgcgttat tgcacagcgt caggttttgc tgaaggtgcg
     4981 cctgcgcgcc gctgctgcgg taaaatttac tggcaccagg catggtgatc agcgtatggc
     5041 aatcagagtc aattattgcg ttgatgatga gttcgtcacc gccgacgata ccaccgggtg
     5101 gatgaagcag acagaggtgg caggtttctt cttcgggata aaaaggacgc tgaacagtga
     5161 gcggaccgac atgatgacgg gtggtgagcg tggttttccc gtgcagacgc tgaaaacgga
     5221 gatccagcat ggcctgccag ccttttttat tttgtggtaa cgcggtactg agcaatctta
     5281 cgcccctgat aaataaagat aaaacgacag taaagagccg gatttccttg ttatataaaa
     5341 gatgatgcct ttccagtgag ttttcgtctg aataatacga atgtttgttt tcgtcatgaa
     5401 gcgttccggc tcccggggct atacatcgcc agaaaagcaa tgcatattgt cgggattatc
     5461 cagagaatca ggcagaaaga tccgacaatg ggctgtgatt atgccatctg gctcagaatg
     5521 atatcaaggt gttatgggga taacttgctg atacaatagc agtatgacag attcattaat
     5581 gcggaaactg ctcactgaaa cagagtgttc atgactgagc actggtcggg tcatgcagat
     5641 gataatttcc ccttttcttg gggaagcgac agtattttca tgcatgcctg tcttcagtca
     5701 tacggggaga gcatctgtga tacagtccat acatttgttg cggacgtcgc tcctgagtca
     5761 ctgactggtc tgcaaacagt ttatctgtac acggcagggc ttctgtgtga tatcgttttt
     5821 catatcgagg gggggcctc
//
LOCUS       DAZGOB010000206         5706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGOB010000206 DAZGOB010000000
VERSION     DAZGOB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.206"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..580)
                     /locus_tag="ACHLIF_004783"
     CDS             complement(<1..580)
                     /locus_tag="ACHLIF_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFO9608629.1"
                     /translation="MDQHFQTKDTPESRRVLEGQIRECYGRVVYSHKTHEKCSDILLS
                     RLSSIKFWQIILSALTTGGFLATFFGAGEVGTGVGIVVSTLLLILNAYTKDYDLGELA
                     QKHKQAANEIWLIREKYLSLLTDLAMGEKPIEQLQSERDSLLESLHSVYSGSPSTTFE
                     AYKKAQDALKNKEDLTFSEEEIDAFLPKELKRR"
     gene            complement(558..1439)
                     /locus_tag="ACHLIF_004784"
     CDS             complement(558..1439)
                     /locus_tag="ACHLIF_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFO9608630.1"
                     /translation="MGLAEWFCGFCSNLTVKDGGTISTRYRNITKRLNTDFWDSSSDT
                     SHSLYVGSYGRNTATQGLSDLDMIFQLPYSEYQKYNSYLGNGQSALLQAVKRSIEKTY
                     ATTSIRGDGQVILVPFNDGITFEVVPAFLNVSDSYTFPDANGGGRWRITNPKPEISAM
                     RLRNNITNNNLVQLCRMARAWKRKWDVPISGLLIDTLAYQFIENWAHRDKSYLYYDFM
                     SRDFFKWMADQDTSKEYWKAPGSGQYVYGKGLFQYKAKRCYNISLEAIEHEMATPKRE
                     WSAKQKWREIYGSTFPD"
     gene            complement(1974..2432)
                     /locus_tag="ACHLIF_004785"
     CDS             complement(1974..2432)
                     /locus_tag="ACHLIF_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9608631.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDRRHNIICTECRKSEVMQRILSFYQGDVRYFFEVTRLKCISSDVN"
     gene            complement(2473..3533)
                     /locus_tag="ACHLIF_004786"
                     /pseudo
     CDS             complement(2473..3533)
                     /locus_tag="ACHLIF_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
     gene            complement(3605..4030)
                     /locus_tag="ACHLIF_004787"
     CDS             complement(3605..4030)
                     /locus_tag="ACHLIF_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9608632.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDAVEKIQALLPAIEKARPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(4014..4256)
                     /locus_tag="ACHLIF_004788"
     CDS             complement(4014..4256)
                     /locus_tag="ACHLIF_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9608633.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            4648..4986
                     /locus_tag="ACHLIF_004789"
     CDS             4648..4986
                     /locus_tag="ACHLIF_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO9608634.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPNRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            5279..5431
                     /locus_tag="ACHLIF_004790"
     CDS             5279..5431
                     /locus_tag="ACHLIF_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9608635.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LTRNTD"
     gene            5443..>5706
                     /locus_tag="ACHLIF_004791"
     CDS             5443..>5706
                     /locus_tag="ACHLIF_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608636.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDTISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFMELMDIINKGFGLLMKKKGIRI"
BASE COUNT         1505 a         1432 c         1159 g         1610 t
ORIGIN      
        1 atcttctttt cagttccttg ggaagaaaag catctatttc ttcttcagaa aatgttaaat
       61 cttctttgtt ttttaatgcg tcctgtgctt ttttataagc ttcaaatgtt gtactaggag
      121 agcctgaata cactgaatgt aaactctcga gtaacgaatc ccgctctgac tgcaattgct
      181 cgattggttt ttcacccatt gctaagtctg taagcaatga taaatacttt tctcggatta
      241 accagatctc atttgcagcc tgcttatgtt tttgcgccaa ctcaccaagg tcataatctt
      301 tggtataagc gttaagaatg agaagcagtg ttgaaacaac aatcccaacc cccgtgccta
      361 cttcacccgc gccgaaaaaa gttgctagaa agcctccagt agttaaggcg gagagaatta
      421 tttgccaaaa ctttatagaa gatagtcgag ataggagaat gtctgagcat ttttcatgcg
      481 tcttatgtga gtaaacgacc cgaccataac actctctgat ctgcccttct aaaacccttc
      541 tagattcagg tgtatcctta gtctggaaat gttgatccat aaatctcccg ccatttttgc
      601 ttagccgacc attcgcgttt tggggtcgcc atttcatgct ctatagcttc gagtgaaatg
      661 ttatagcacc gttttgcttt atattggaaa agccctttac catagacata ctgaccactt
      721 cctggcgctt tccaatactc cttactcgta tcttgatcag ccatccattt gaagaaatca
      781 cgactcatga aatcgtagta aagatacgac ttatctctat gcgcccaatt ttcaatgaat
      841 tgatacgcaa gcgtatcaat gagtaaacca cttataggta catcccattt acgcttccaa
      901 gctcttgcca ttctacaaag ttgcaccaaa ttgttattgg taatgttatt tcttaacctc
      961 attgcagaaa tttctggctt tggatttgta atcctccaac ggccaccacc gttcgcatca
     1021 ggaaatgtgt aactatcact aacattgagg aaagctggaa cgacttcaaa tgtaattcca
     1081 tcattgaacg gtacaaggat cacttgaccg tcacctctaa tactagtggt agcataggtt
     1141 ttttcaatag atctcttaac cgcttgaagc aatgctgact gcccatttcc taagtagcta
     1201 ttgtattttt gatactcgga atatggtagc tgaaaaatca tatccagatc gctcaagcct
     1261 tgcgtagctg tgttccgacc atatgatcca acgtagagac tatgagaagt atcagaactg
     1321 ctatcccaaa aatccgtatt taaacgcttc gttatattcc tatatctagt tgaaattgtt
     1381 ccaccatctt ttacagtaag attggagcaa aatccgcaaa accactcagc taaacccatg
     1441 ctcaattcct tttctaaaat ctaatgaaaa attcaagggc gcatgttcca tgcatcattg
     1501 ataacttttt tcagtcagct agagcaacat aattgatact aactagctgt ttcttcaagt
     1561 gagtaaccta ctttagcatc aaggtattat acctaccaag catggggaga aaccaccact
     1621 taagccattg aagtatatag agcagatttt gttgaaaaag cactgtttgt ctatacatat
     1681 gaatatgtgc ttttaaccag caatagcgcg gctctcagcg ttttacaagc agtgtcatgg
     1741 ggtgtcgggg atcggaagtt caaatcctct cgtgcgaccc aaaagattat ataaaccagc
     1801 ctgttattgc tggtttttta tgcctacctt cctggtaggg aagctatggg gaattactgg
     1861 ggaatccccc ccctttacct gctcccatta aactaacata gcacgatgta agcaacttcc
     1921 gcttctggca caaagcggac aaccacgcta gctctaccct gtgccacaaa atgtcaattt
     1981 acatctgaac taatgcactt taatctcgtc acttcaaaaa aataccgaac atccccctga
     2041 taaaacgaca atatccgctg cataacttcg cttttacgac actcagtaca aattatatta
     2101 tgacgcctgt cgtaacgacg tatttctcca tcaggtaatg accagataag gtccggatca
     2161 accgcagatg gtttcttcag ctttgccctt gagagctttt tacgggcatt ttgccagtcc
     2221 ttacgcgcct gttcagacgg gaataacccg taaccagagt tgtatacatc gccactggca
     2281 accagctctc tggccagaac gctcatcaga tatcttgttg ccccagtttt agcttccagt
     2341 tgtcgtaacg tctcgcgccc actctggcgt acgagttcaa gaacctgccc tttaattttt
     2401 tcccgctctt cttgtgtaaa aacttttgcc acaagccctc ctgaaaatta cctcatgacc
     2461 agaaatacac acttaccccc tgaagcccgg tggaatttcg gtatccggtt cagaaatatg
     2521 attcacacaa cgctggttgt tcgtaccgct taccgggagc aaccaggggt tttcaaaatt
     2581 ccggtccggt ccaaaaaacg tcgtcgctcg ctgaacaaat tccgttcccg ttttcccggt
     2641 agccgccagg tatcttgcgt aacgcctcac accatccagc atggcctctg gtggcacccc
     2701 ctcgcgtaat ctggccttcc aggcactgaa agcggatttc ttcgggtttg ccccagcacg
     2761 caacgggtat tcccgccaga cctgttcgaa cacatccgga taatctactc gccccacagg
     2821 ctgcccggtg ttttccggga ctacccgatc ggcttcccgc tgaatggcgg aatcggcttc
     2881 aggctgctgc agttggtgtg attgctccgg ccttgcggtc atcacctgct gcacagcgcc
     2941 cgaatcggct ttcagcgcat acgctgaatc ggcttccggt gtcgtgcctg ctggctgacc
     3001 aggattgacg gtctgaacat cccctgcctg gttcgtggcg ttttttacgc catggaccat
     3061 agtgttttga tcttcttgat ctgtatcttt atctgtatct ttatctgtcg tgacatgtgc
     3121 gtgacatttc gtgacgcgcc gtgacaatcg ccattttgtt cccgctttct ttccctctct
     3181 cgctgcgccc tcttgcgctc tgcaggagat tttgcggttt gcgaaatatt gccgttgtcc
     3241 tctttaagca cctggcgttt ttcccatcca gtgattaaat caccatcaag tacccgcccc
     3301 tgcatcgtct gcaaaattga atcaattacc tcttctgtca cgtcgagcgc acttgccaaa
     3361 tcttctgtcg tgacatcaat gtgacctcgc gtgacatttc gtgacgcgct caccaggagg
     3421 tggatataca ctgccatcac tgttgcaatt ggctgccctg acaccctggc aattgttcgc
     3481 cacttagggt catttggcat gtcatgccat aatctgagcc aagcgttagc catactcacc
     3541 tcttctgata ccgaatcttt ttactcacga gttgccggaa gcgattcgat atggctattg
     3601 tcagtcaatg tactgccaca gcatttcctg ccgggccacc acggttcatc tgattgaaac
     3661 cggcgattgc cactgcgaca aaatcatcag cgtctctcac cagtcgctcc cgcgtctcca
     3721 ccagctcccg aaaataagct gaactgtggc tgcgcattct ggccaccagc aaaggtggcc
     3781 ttgccttttc gatcgctggt aacaacgcct gaattttttc aactgcatca ggggtatctt
     3841 tctctatcca gcggaaaatt ttctgggtat tgcgagccag ggcttccgga tggctgtcgt
     3901 cgtacagttc aggaaacgtc atacccaact caaaataagc ctgggttatt ccagctgccg
     3961 gaactttttc gccatcagga cgcgcccagg cattcatcgc catgcggatg tgttcatgct
     4021 tgattttcat gaatcccccc ttggttagaa ggcggattat gatcagaacc gggaatgaca
     4081 accgtcggta tgtgtaactc atatttgagc gccccggcag tgactgcctg aattagcaac
     4141 gcccatttcc acggaacctc ttccccccac atgctgactg tggtttttga cgttcctaga
     4201 gctgcggctg ttttaacaac tccgccaaaa tagcctaata cttctgattt tttcatgagt
     4261 cgctccataa aactgaacgc caaaagttta ataatcaaaa ccaaagaaag tcaagaaaca
     4321 aaaccatctg tgttttaaaa tcaaaacatg agcaagcaaa caatatctga acgcataacc
     4381 caacgtatgc atgcgctaaa cctgaaaggc aaagaccttg tcaatgccac tggcgcatca
     4441 aaaggctccg taagtcaatg gatgaacggt ggaggagcgc cgtcctcgcg ttacataagt
     4501 tcactggcaa aaatattgaa agtaaacgaa aattggcttc ttaatggagg agagttaaat
     4561 acaggtgatt cgcttgatct atctttaccg ccgataaaaa cggttccgct actatcactt
     4621 cagcaggcag caagctggag tgattatatg aaaaattcct caataacctc ttgtgtgcag
     4681 cttgtcggag aaatcccggc caataccttt gcagttgttc tagagagtga cagtatgtca
     4741 acatctggtg ggggagtttc catcccaaat ggttcaacag tttttgttga tcccaatcga
     4801 accgtacaac caggaaatat tgtccttgcc ttacccaaag ggaccacaac acctgtcatt
     4861 cgtaaactgg agatagaagg gccggatatt cttttagtcc ccacgaatcc tcgctaccct
     4921 tcaattatgc tggatgatct atcttgcata ttgggcgtat gctttaaaat tcaacaagat
     4981 atttaaccga cctcatctat ttgattaact gtatgccatc gtggtgatgg cttaacagct
     5041 gcctgcttaa aatgttttga taaaaaaaca ttgacctgaa aagttcattt ttctaaactt
     5101 cattcattcc ctctccccac cccacagaat gcagggcaat acttcgagtt accaggcagt
     5161 ggtcaggggt taagtagcca gcccgaggcg taagaacatg acggcagggt tcaactttaa
     5221 taactatgca gcaggttttt gttccgctac cccggcgtta aggggaaatg aggtcagcat
     5281 ggatactatc gatcttggca acagcgaatc tctggtatgt ggcgtgttcc ccaaccagga
     5341 cggtacgttc accgcgatga cgtataccaa aagcaaaacg tttaaaactg aagctggcgc
     5401 gcgtcgctgg ttaaccagaa acactgactg atgaggttga cgatggaatt taaagattta
     5461 ccagtaccat tccaggaaat ggcatcgaat gtggttcgct ctcaactggc gactcttgac
     5521 ctgagtaccg tagaaaaaga aaccatcgat actatatccg gtaacgtgcg tcgtgccttt
     5581 atcggtctgt acgaagagaa gcagctctct gataaccagg atttacatga aaaatacttc
     5641 atggaattaa tggacatcat taataaagga tttggcttgt taatgaaaaa gaaagggatt
     5701 cgaata
//
LOCUS       DAZGOB010000207         5684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGOB010000207 DAZGOB010000000
VERSION     DAZGOB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.207"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            146..856
                     /locus_tag="ACHLIF_004792"
     CDS             146..856
                     /locus_tag="ACHLIF_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9608637.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            898..3549
                     /locus_tag="ACHLIF_004793"
     CDS             898..3549
                     /locus_tag="ACHLIF_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9608638.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVITVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            3563..4093
                     /locus_tag="ACHLIF_004794"
     CDS             3563..4093
                     /locus_tag="ACHLIF_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9608639.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4106..4609
                     /locus_tag="ACHLIF_004795"
     CDS             4106..4609
                     /locus_tag="ACHLIF_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9608640.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4668..5582
                     /locus_tag="ACHLIF_004796"
     CDS             4668..5582
                     /locus_tag="ACHLIF_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9608641.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1640 a         1235 c         1267 g         1542 t
ORIGIN      
        1 tttcctatta aaagagaacc aacatttatt acaaacgcat taagtctgga aacccggcga
       61 ctcgccaatg tattagcaac attaatcgat tgtctgatgt aacggttttc ccgcttatcc
      121 tcttaacaca tggagaatgc atcagatgca aactacgcgt accccctact ctatttcctt
      181 tatggcgaca gtgttacttt tattgctctt tgcttgccat agcaccgttg ccaacgcagc
      241 tgtcgcactg ggtgcgacgc gggtaattta tccggcaaac cagaaacagg ttttattgcc
      301 tgtcacgaat aatgatcctg ccagtgtcta tctcattcag tcatggatag aaaatgcagg
      361 tgaccaaaaa gacacccagt ttgtcattac cccaccgctt ttttccatgc aaggtaaaaa
      421 agagaatacg ctgcggataa tcaacgctac taatcatcag ttaccaggcg accgtgaaag
      481 cctgttctgg gtgaatgtta aagctattcc tgctatggaa aaagaccaga agaatgagaa
      541 tacattacag ctggcaatta ttagccgcat aaaaatgttt tatcgcccca cgaacctggc
      601 aatggcacct gaagaagcgc ctgcaatgct tcggtttcgt cgcagcggta gcaaactcac
      661 gcttattaat ccaacgccct atttcattac cgtaacaaat atgaaagcag gaaatagcaa
      721 tctcccaaac accatggtgc cgccaaaagg tgaagtatcg gtggatatca cgcatgctgc
      781 aaccggggat atcagttttc agacgatcaa tgattacggt gcattaactc cgcgtattaa
      841 ggcaaccatg cagtaagcca gtgacctaat taataataaa ctcaatcgga attaataatg
      901 acagcctttc gtgccgcatt taaggcatat cgtatgcatc aggtgttaat aatgcctcgc
      961 tttgcacggc ttaccattgc ccttggctta gcaactgcgg tatttcccgt tgacgcagag
     1021 tattatttta atccacggtt tttaagcaat gatcttgctg aatctgtcga tttatccgcc
     1081 tttactaagg gacgcgaggc accacctggt acatatcggg tcgacattta tttgaacgac
     1141 gaatttatga ctagtcggga tattactttt attgcagatg ataataacgc agaactcatc
     1201 ccatgcctga gtacagatct tcttgtcagt cttggtataa aaaaaatagc gttattagac
     1261 aataaagaac attcggcaga aaaacatgtg ccggacaaca gcgcatgcac accacttcag
     1321 gatcgtttgg ccgacgcatc aactgagttt gatgttggtc agcaacattt gtcactctcc
     1381 gttccgcaaa tttatgttgg cagaatggct cgcggctatg tttctcctga tctgtgggaa
     1441 gaaggaataa atgctgggct tctaaactat agttttaatg gtaattctat taataatcgt
     1501 agtaaccata atgcaggaaa atccaactat gcatatttga atttacagag tggcatcaac
     1561 attggtagtt ggcgactgcg cgataactca acgtggagtt ataacagtgg gagtagcaat
     1621 tcatctgaca gcaataaatg gcagcatatc aatacgtcgg ctgaacgtga cattattccc
     1681 ttacgctcac ggttaacggt aggtgatagt tataccgatg gcgatatttt tgatagtgtg
     1741 aactttcgtg gcctcaaaat aaattcaaca gaagcgatgt tgcccgatag ccaacatggt
     1801 ttcgctccgg tgattcatgg tattgcccgt ggaaccgcac aagtgagtgt aaaacagaac
     1861 ggatacgatg tttatcagac tactgttcca cccggccctt ttactattga tgatatcaac
     1921 tctgcggcca atggtggtga tctgcaagta accataaaag aggcagacgg cagtattcag
     1981 acattatatg ttccttattc gtctgttccg gttctccaac gtgctggata tacgcgttat
     2041 gcgcttgcca tgggggaata tcgtagtgga aataacctgc aaagctctcc caagttcata
     2101 caaggtagct tgatgcatgg tctggaagga aactggacac cttatggcgg aatgcaaatt
     2161 gcagaagatt atcaggcctt caaccttggt attgggaaag atttaggact ttttggtgcc
     2221 ttttctttcg atatcacgca ggccaatacg acacttgcag atggcacccg tcacagtggg
     2281 caatcggtta aatccgtcta cagcaaatcc ttctaccaga caggaaccaa tatccaggtc
     2341 gcaggatatc gctattctac gcaaggtttt tataacttat ccgacagtgc ctacagtcga
     2401 atgagtggtt acaccgtcaa gcctcctacc ggagacacca atgagcagac acaatttatt
     2461 gattatttta atctgttcta cagtaagcgt ggtcaggaac aaataagcat ctctcagcag
     2521 cttggaaatt acggtacgac atttttcagt gccagtcgcc aaagttactg gaacacgtca
     2581 cgcagcgacc agcaaatatc atttggatta aatgtgccgt ttggtgatat tacgacttcg
     2641 ctgaattaca gctattccaa taatatatgg caaaacgatc gggatcattt actcgctttt
     2701 acgcttaatg ttcccttcag tcattggatg cgtacagaca gtcagtcggc atttcgtaat
     2761 tcaaacgcca gttacagtat gtcaaacgat ttgaaaggcg gcatgaccaa tctatcgggg
     2821 gtttatggca ctctgctgcc ggataataac ctgaattata gcgttcaggt cggtaacacc
     2881 cacggaggta atacatcgtc tggcaccagt ggttacagtt ctcttaatta tcgtggagct
     2941 tatggtaata ctaatgtcgg ttacagtcgg agtggtgaca gcagccagat ttattacgga
     3001 atgagtggtg ggattattgc tcatgctgat ggcatcacct ttggacagcc gctgggcgac
     3061 acaatggttc tggttaaggc tcctggtgct gataatgtca aaatagagaa ccagaccgga
     3121 attcataccg actggcgtgg ctatgccata ttaccatttg cgacagaata tagagaaaac
     3181 cgtgttgctc ttaacgcgaa ttcccttgca gataatgttg aactggatga aaccgtgatc
     3241 actgtcatcc caactcacgg tgctattgcc agagcaacat ttaatgcaca aatcggcggg
     3301 aaagtattaa tgacgttgaa gtacggtaat aagagtgttc cattcggtgc aattgtcaca
     3361 cacggagaga ataaaaatgg cagcattgtc gcagaaaacg gtcaggttta tctgactgga
     3421 cttccacagt cagggaaatt acaggtttca tggggcaaag ataaaaactc aaactgtatt
     3481 gtcgagtaca agcttcctga agtttctcct ggtaccttac tgaaccagca gacagcaatc
     3541 tgtcgctaag gaacaataga ttatgaaata caataacatt attttcctcg gtttatgtct
     3601 ggggttaacc acctattctg ctttatccgc agatagcgtt attaaaatta gcgggcgcgt
     3661 cctcgattat ggctgcacag tctcatcgga ttcgcttaat tttaccgtag atctccaaaa
     3721 aaacagtgcc agacaatttc caacgaccgg tagcacaagt ccagccgtcc cttttcagat
     3781 tacgttaagt gaatgcagca aagggacaac gggggttcgg gttgcattta acggtattga
     3841 ggatgcagaa aataatactt tgttgaaact ggatgaagga agcaatacgg cctccggttt
     3901 gggtatagaa atattggacg caaatatgcg tccggtgaaa ctgaatgacc ttcatgccgg
     3961 gatgcagtgg atcccactgg taccagaaca gaacaatatt ttgccttact ccgctcgtct
     4021 gaagtcaact cagaagtccg tcaatccggg actggtgagg gcttcggcaa cctttaccct
     4081 tgaatttcaa taatcagagt cagatatgaa aagacttcac aagaggttcc tgttagctac
     4141 gttttgcgcg ttattcacag caactctcca ggccgccgat gtcactatca ctgttaatgg
     4201 tcgggtagtc gctaaaccct gcactattca aaccaaagaa gctaacgtta atctcgggga
     4261 tctttatacg cgcaatctgc aacaacctgg ttctgcatct ggctggcaca atattacttt
     4321 gtcattaacc gattgtccgg ttgaaacaag tgcagtgacg gcaatcgtga caggttcaac
     4381 tgacaatacg ggttattaca aaaatgaagg tactgccgaa aatattcaga tagagctgag
     4441 ggatgaccag gatgctgcgt taaaaaatgg cgatagcaaa acggttattg ttgatgagat
     4501 cactcgtaat gcacagtttc cacttaaggc aagagctatc acggtgaatg gaaacgcaag
     4561 ccagggaacg atcgaggcgc taatcaatgt gatctacacc tggcaataac gtcgattcga
     4621 ttttcttagc cttcttttta aatcaatttt taaccttagg atgtatcatg ggtaaaacaa
     4681 tcagtataaa ggtcctgttc ggtatttatc ttctattaat ggcaggcaaa gtttttgcct
     4741 tcagttgtaa tgtcgatggc gggtcaagta ttggagcagg tacaacctct gtttatgtta
     4801 atctcgaccc tgtaatacag ccgggccaga atctggttgt agacttgtct cagcatataa
     4861 gttgctggaa tgattacggc ggctggtacg acactgatca tataaacctg gtacaaggct
     4921 cagctttcgc aggctcccta cagtcatata aaggatcgct gtactggaat aatgtgacct
     4981 accccttccc gttgacgaca aataccaacg tattggatat tggtgataag acaccgatgc
     5041 cattaccgtt aaaactctat attaccccag ttggggcggc cggtggagtt gtcataaaag
     5101 cgggggaagt cattgcccgt atacatatgt ataaaattgc aacattagga agcggaaatc
     5161 cgcgtaattt tacgtggaat attatttcaa ataacaatgt tgttatgcca acaggcggct
     5221 gcaccgttga tagtcgtaat gtcaccgtgg atctcccgga ctttcctgga agcgcagaaa
     5281 taccccttgg cgtatattgc tcaagcgaac aaaagctctc attttatctt tcgggtgcca
     5341 ccactgacag tagccgacaa gtgtttgcta acacagcacc tgatgcaaca aaagcatcgg
     5401 gagtgggtgt cactctaatg cgtaatggaa aaatactggc tacgggagaa aatgtctccc
     5461 tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag
     5521 gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat
     5581 aattgtgatt ttgaccttag tctatgcgat ccattcacga aaattgcgcg gataattatt
     5641 ttgtgaaggc tattagccta cacctgtaag aaaatccgcg caca
//
LOCUS       DAZGOB010000208         5679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGOB010000208 DAZGOB010000000
VERSION     DAZGOB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.208"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..262)
                     /locus_tag="ACHLIF_004797"
     CDS             complement(<1..262)
                     /locus_tag="ACHLIF_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608642.1"
                     /translation="MENVRFLQGVKQYFCTKALDVILLIYLFLGFILLPYKYLWKDII
                     LSLCFVGILLYALVEENRITEYMGYFVKFSNKNRLNSCAAWCA"
     gene            403..2835
                     /gene="hsdR"
                     /locus_tag="ACHLIF_004798"
     CDS             403..2835
                     /gene="hsdR"
                     /locus_tag="ACHLIF_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO9608643.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKAYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2902..4371
                     /locus_tag="ACHLIF_004799"
     CDS             2902..4371
                     /locus_tag="ACHLIF_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO9608644.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     VLELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMKTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRIENEQAWKVSIDDVIARNFNLDIKNPHQAETI
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4371..>5679
                     /locus_tag="ACHLIF_004800"
     CDS             4371..>5679
                     /locus_tag="ACHLIF_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO9608645.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWTRLINLGIWALGSGFPNVVQGSTDKEILMCKVSDMNLEGNEKFIFSTKNTISK
                     DLADEYKIKISEPGTIIFPKIGGAIATNKRRILVQDTAIDNNCLGIKPCDAISGEWFY
                     LILNTLDMSKYQSGTSIPAINQSVIGSIPIALPSLKMQEKIVSYVITLMSLCDQLEQQ
                     SLTSLDAHQQLVETLLGTLTDSQNAEELAENWARISEHFDTLFTTEASVDALKQTILQ
                     LAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEG
                     WEWCKFGLTSEFINGDRGSNYPNKNEYVSQGIPWINTGHIEKNG"
BASE COUNT         1720 a         1339 c         1339 g         1281 t
ORIGIN      
        1 gtgcacacca ggcagcacaa ctattcaacc tgtttttatt actgaactta acaaaatacc
       61 ccatatattc tgtaatgcga ttttcttcca ctaatgcata aagaagaata ccaacaaagc
      121 acagagataa aataatatct ttccacagat atttataagg gagaagtata aaaccaagaa
      181 atagataaat tagcagaatg acatctaatg ccttagtaca aaaatattgt ttcactcctt
      241 gcaagaaccg aacattctcc atatgaatcc ttatcctact attgatgaaa ctcaagaata
      301 cctttaaaac agtgacttaa cgctgataat actctttgaa cagatgaatt catctatttt
      361 tctgtgtaga atttgcgcat cgcatttaga tgagaacacc ccatggcgga actgaaccta
      421 agtaacctga cggaagcaga catcattacc aaatgcgtta tgccagccat tctcaatgca
      481 ggctgggaca acacaacgca aatcagacag gaggtcaaac tccgggacgg taaagtcatc
      541 gtacgtggta aagttgcggc acgcagaacg gtaaaatctg ctgacatcgt gctgtatcac
      601 aaaccaggca ttcccttagc cgtgattgaa gcaaaagcca acaaacatga aattggcaaa
      661 gggatgcaac agggcattga atatgcgcgc ctgctggacg ttccttttgt ttttgccacc
      721 aacggggatg gctttatctt ccgcgatgcc accgcagccg aaggtgaatg ccttgaaaag
      781 caaataacgc tggatgactt cccctccccc gctgaactct ggcaaaaatt ctgcctctgg
      841 aaaggataca cacaagctca gcttccggtg attactcagg actattacga cgatggcagc
      901 ggtaaatcgc cacgttatta ccagcttcag gcaatcaaca aaaccattga agccgtctcc
      961 aacgggcaaa accgcgttct gctggtcatg gcgaccggaa cagggaaaac ctataccgca
     1021 ttccagatca tctggcgcct gtggaaatca aaaaataaaa aacgcatttt gttccttgcc
     1081 gatcgcaaca ttctggtcga ccaaaccaaa aataatgatt tccagccatt tggtacggca
     1141 atgaccaaag tcagcggacg caccattgat cccgcttatg aaattcacct cgcgctctat
     1201 caggctataa ctggcccgga ggaagaccaa aaagcgttta aacaagtcgc accagatttc
     1261 ttcgatctga tcgtgatcga cgaatgccat cgcggcagcg catccgaaga cagcgcctgg
     1321 cgagagatcc ttgattactt cagttccgcc acccaaattg gcttaaccgc cacgccaaaa
     1381 gagacgcatg aagtctccag cacggattac ttcggcgatc cggtttacgt ctactcgcta
     1441 aaagaaggga tcgaagacgg cttcctcgcc ccttataaag ttgtccgtgt tgatattgat
     1501 gttgatctgc aaggctggcg cccaaccaaa gggcaaactg acttaaacgg cgaagtgatc
     1561 gacgatcgta tctataacca gaaagatttc gatcgcacga tggtaatcga cgaacggaca
     1621 gaactggttg ccagaaccat taccgactac ctcaagcgca ccaacccgat ggataaaacc
     1681 atcgtcttct gtaacgacat cgatcatgca gaacgtatgc gccgcgccct ggtgaatctc
     1741 aacccggagc aggtgaaaaa gaacgacaaa tacgtcatga aaatcaccgg cgatgatgaa
     1801 attggcaaag ctcagttgga taacttcatc aacccgaaaa aagcgtaccc ggttatcgcg
     1861 accacttcag agctgatgac cactggtgtg gatgctaaaa cctgcaaact ggtagtactg
     1921 gaccagaaca tccagtcgat gaccaaattc aagcaaatta tcggtcgtgg tacacgcatc
     1981 gacgaacgtt acggcaaact ctggtttacc atcctcgact ttaaaaaagc caccgaactg
     2041 tttgccgatg agcgtttcga tggcattccc gaaaaagtca tggataccac accagaggat
     2101 atcgccgatc cagaatctga ttttgaagag aaactcgaag aaatcagcga acatgacgac
     2161 gaacaggtaa caggcgttga tgaaccgcct gcgccaccat accaggttac cgataccgat
     2221 gatgtcggcc cacttccgga agaagacgag aagaaaatcc gcaagtttca cgtcaacggt
     2281 gtagcggtgg gcgttattgc gcagcgtgtt cagtattacg atgccgacgg taaactggtt
     2341 accgaatctt ttaaagatta cacccgcaaa acactgctca aagaatatgc ctcgctggat
     2401 gactttaccc gcaagtggca ggacgccgat cgcaaagaag cgatcattca cgagctggag
     2461 caacagggga tcatctggga agtactggca gaagaagtcg gtaaagatct cgacccgttc
     2521 gacatgcttt gccacgtagt gtatggtcag ccgccgttaa cccgcaaaga gcgcgccgag
     2581 aacgtgcgca agcggaacta cttcacaaaa tactctgaag cagcgcaagc cgtgctcgat
     2641 aatctgctgg ataaatacgc cgatgcgggc gtacaggaga tcgaaagtat tcaggtgctg
     2701 aaacttaaac cattcgacag catgggcact ttaccggaga ttattaaaac cggatttggc
     2761 gaccgtaacg ggtataatca ggcgctcagc gagctggaaa acgaaatcta ccaattaccg
     2821 ccccgctctg cttaacaact ctcttttgtg ccttctgcaa ccaacatgtt gcagaaggcc
     2881 ttgtttttca tggaatacaa catgtctatc agttcagtaa tcaaatcatt acaagatatt
     2941 atgcgcaaag atgccggtgt ggatggcgat gcgcagcgtc tcggtcagct ctcctggctg
     3001 ctgtttttga aaatcttcga tgcccaggaa gaggtgctgg aactggagca ggataactat
     3061 caatatccga tcccacagcg ttatttatgg cgcagttggg ccgcaaacgc tcagggcatt
     3121 accggtgatt ccctgctgga attcgttaat gatgatctgt tcccggcgtt gaaaaacctc
     3181 actgcgccta tcgataaaaa cccacgcggc tacgtggtga agcaggcgtt cagcgatgcc
     3241 tataactata tgaaaaacgg tacgctactg cgccaggtga tcaacaagct gaacgaaatt
     3301 gactttacca gcgccagcga acgccatctg tttggtgata tttacgaaca gatccttaaa
     3361 gatctgcaat ctgcgggcaa tgctggcgaa ttctatacgc cacgcgccgt cactcgcttt
     3421 atggtggatc gcgttgatcc gaaactcggc gaatccatta tggacccggc ctgcggtacg
     3481 ggcggttttc ttgcctgcgc atttgatcat gtaaagaaca aatacgtgaa gagcgtcgcc
     3541 gatcatcaga cgctgcaaca acagatccac ggtgttgaga aaaaacagct tccgcacctg
     3601 ctggcgacca ccaatatgct gctgcacggc attgaagtac cggtacaaat tcgtcacgac
     3661 aacaccctga acaaaccgct ttcctcctgg gatgaacaac tggatgtcat tgttaccaac
     3721 ccgccgtttg gtggcacgga agaagacggt attgagaaga actttccggc agagatgaaa
     3781 acccgcgaaa cggcggatct gttcctgcaa ctgattgtgg aagtactggc gaaaaacggt
     3841 cgcgcggcgg tggtattgcc ggatggcaca ctatttggcg aaggcgttaa aaccaaaatc
     3901 aaaaaactgc ttaccgaaga gtgcaacctg cataccatcg tgcgtttacc gaatggggtg
     3961 tttaacccct ataccggcat taaaaccaac ctgttgttct ttaccaaagg tcagccaacc
     4021 aaagagattt ggttctatga gcatccgtat ccggcgggcg taaaaaacta cagcaaaacc
     4081 aagccgatga agtttgaaga gtttcaggcg gagatcgact ggtggggtaa cgaggccgat
     4141 ggttttgcca gccgcatcga gaatgagcaa gcgtggaaag tcagcattga tgacgtgatt
     4201 gcgcgtaact tcaatctgga tattaaaaac ccacatcagg cggaaaccat tagccatgat
     4261 ccggacgaac tgttagcgca gtatgcaaaa cagcaggcgg agatccagac gctgcgtaat
     4321 caactgcgcg atattcttgg cgctgcgctg tctggcaagg aggttaacta atgagtgtgg
     4381 aaaagctgat cgttgatcat atggaaacct ggacctcggc gttgcaaacc cgttccaccg
     4441 ccgggcgcgg cagctccggt aagattgatt tgtatggtat taagaaattg cgtgagctga
     4501 ttctggaact ggcggtgcga ggtaaactgg tgccgcagga tccgaacgat gaaccggcgt
     4561 cggagctgct gaagcgtatt gcggcggaaa aagcagagct ggtgaagcaa ggtaaaatta
     4621 aaaagcaaaa accgctgccg gaaattagcg aggaagagaa gccgtttgaa ttgccggagg
     4681 ggtgggaatg gactcgatta attaatcttg gtatatgggc tttagggtct ggttttccaa
     4741 atgtggtaca gggaagcact gataaagaaa ttttgatgtg caaagttagc gacatgaatc
     4801 ttgaaggtaa cgagaaattc attttttcga caaaaaatac aatttcaaaa gacctagctg
     4861 atgaatataa aattaaaatc tcagaacccg gcaccatcat tttccctaaa attggcggag
     4921 caattgcaac taataaacga agaattttag ttcaggatac agctattgat aacaactgtc
     4981 ttggaataaa accatgtgat gcaatatcgg gggagtggtt ctatctaatc cttaatactc
     5041 tcgatatgtc gaagtatcaa tcaggaacct caatacccgc gataaatcaa agtgtcatag
     5101 gtagtatacc aattgcgctt ccttctttaa aaatgcaaga gaagattgtt tcttatgtca
     5161 taaccctaat gtccctttgc gaccaactgg aacagcaatc cctgaccagt ctggacgcac
     5221 atcagcaact ggttgaaacc ctgttgggaa cacttacaga cagccaaaac gccgaggaac
     5281 tggctgaaaa ctgggcgcgt attagcgagc atttcgacac cctatttacc accgaagcca
     5341 gcgtggatgc gttaaaacag accattctgc aactggccgt aatgggcaaa cttgtgccgc
     5401 aggatccgaa tgacgaacca gcctctgaac tgctcaaacg aattgcgcag gaaaaagctc
     5461 aactggtgaa agaaggaaaa ataaaaaaac aaaaaccgtt gccgccaatt agcgatgagg
     5521 aaaaaccgtt tgaacttccg gaagggtggg agtggtgtaa atttggttta acatcagagt
     5581 ttataaatgg cgatcgtgga agtaattacc ccaataaaaa tgagtatgtt tctcaaggta
     5641 ttccttggat caatactgga catatagaaa aaaatggta
//
LOCUS       DAZGOB010000209         5606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGOB010000209 DAZGOB010000000
VERSION     DAZGOB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.209"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(210..1400)
                     /locus_tag="ACHLIF_004801"
                     /pseudo
     CDS             complement(210..1400)
                     /locus_tag="ACHLIF_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131474.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1664..1864)
                     /gene="torI"
                     /locus_tag="ACHLIF_004802"
     CDS             complement(1664..1864)
                     /gene="torI"
                     /locus_tag="ACHLIF_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFO9608646.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(1922..2089)
                     /locus_tag="ACHLIF_004803"
     CDS             complement(1922..2089)
                     /locus_tag="ACHLIF_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608647.1"
                     /translation="MHFRVTGELNGEPFNRVIEAEDINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(2125..2424)
                     /locus_tag="ACHLIF_004804"
     CDS             complement(2125..2424)
                     /locus_tag="ACHLIF_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608648.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            complement(2582..2860)
                     /locus_tag="ACHLIF_004805"
     CDS             complement(2582..2860)
                     /locus_tag="ACHLIF_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO9608649.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGYVIVVTTQSGMTIQIHKPKEEK"
     gene            complement(2860..3447)
                     /locus_tag="ACHLIF_004806"
     CDS             complement(2860..3447)
                     /locus_tag="ACHLIF_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9608650.1"
                     /translation="MTISTKKPLYAEVHNVPDDYEFTDEELNRIIAGDMFTPRQDAIM
                     AREIQKLRAAMLHGAEPVSQTYKSQHTQFEQVADLYEMQFDDGRTCAFHTDAQKAAQW
                     LQACDGNRVQEYVKLERLRNALSGNSPVTPDGWICCSERMPDKLIPVMVMYEDGEMWS
                     AMWNGNRWDDGTEYPDPHSVTHWREMPAAPQQEVK"
     gene            complement(3444..4061)
                     /locus_tag="ACHLIF_004807"
     CDS             complement(3444..4061)
                     /locus_tag="ACHLIF_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608651.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPIGEVSEKRLGLVMDGTVDLGGKSTYRIIKGEKAMKLLPLGTKFYTAPPAPIALEA
                     IENAIEYIRSIAFHIDEDDYHGKHIAYFMRQALAWLEGHSCSDDSQGKSDNSPLPRYQ
                     VIELTMLVKQLVSQLKKAKPDCKLPDRAMDYLLRNGLVSAEDVLR"
     gene            complement(4065..4253)
                     /locus_tag="ACHLIF_004808"
     CDS             complement(4065..4253)
                     /locus_tag="ACHLIF_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608652.1"
                     /translation="MSLTVRQLISKLRKMPPEAVVVWQGYDQSEGEYNDFVGHVADVT
                     DENAPSFDPEVRVVALRG"
     gene            complement(4255..4923)
                     /locus_tag="ACHLIF_004809"
     CDS             complement(4255..4923)
                     /locus_tag="ACHLIF_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9608653.1"
                     /translation="MSLIDYQALRAKAEKATCGVWSLEYGESRFDCDDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDELERNQQYIKRRDQE
                     NEDIALTVGKLRVELEGKDKLIAELGKQCAEWERKALSNFEECAAMAERIEEMSKQSC
                     EARERDLFESWVMHSICISKSTLEGLRTETGYRNATLSGTDFNRMWKQWKSIRATGIR
                     IKGE"
     gene            complement(5400..5507)
                     /locus_tag="ACHLIF_004810"
                     /pseudo
     CDS             complement(5400..5507)
                     /locus_tag="ACHLIF_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5504..5606)
                     /locus_tag="ACHLIF_004811"
                     /pseudo
     CDS             complement(5504..5606)
                     /locus_tag="ACHLIF_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
BASE COUNT         1362 a         1378 c         1171 g         1695 t
ORIGIN      
        1 agctccagtt aatagagcct gaagtaattt cttagtattt catcataagc gtttatctat
       61 cgtctctttc taaattaacc ggtcgtatta caaggtgctg aatctgatta aggaatgata
      121 cttaggggat tatggctgac ttaataatta gataactgat aaatgactat cagggggata
      181 tcgttaagtc agcctttaaa atcaagcgat tatatagctg gtgactcttt tcgaagagca
      241 tcaaggtagt cagcgtacca ttgcatcatt tctcgtcttc cttcaaggta acgagcatgg
      301 ttgtatgtac cacgaatgct gtttttgtcc gtatgtgcaa gttgcatttc aatccatgca
      361 ctattgaatc cttgctcgtg tagaacagta ctcattgtat gacgaaatcc atgcccggta
      421 gcttttttgt catagccaat cttcttcaaa accatattga cgcttgcatc gctgataggc
      481 ctgttaacat catttcttcc ggcaaagatg tatctgtatc ttccagtcag ttctttgagg
      541 atttgcagag actggattgc ctgatctgac agagggacga tatgctcctt cctcattttc
      601 atccgggtcg gaggaacttc ccatatcttt tgggtaaaat cgatctcaca ccattccccc
      661 atacgtaatt ctactgttct cagccctgtg agcatcaaca attttgtagc ttcacgggtt
      721 acaatactgc cggtttagtt ttcgagtttg tataaaaact ctggtagttc aggaggaagc
      781 aggaaagggt aatgtgttcg cttatagctt ttcattgcta taaccagatc agaagctgga
      841 ttgtatcgtg cccgacctgt aacaatggca taccgaaaca cttcaccaca tcgttggcga
      901 actttattag ctagctcgca ggctcctcgg ctttcgatac gctgaagaac tgcgagaagt
      961 tcaagaggtt cgatttcact aatgggtctg tttccaatgt agggaaacac attatttttg
     1021 aatgctctgt cgacataatc agcgtagctg gcagaccagc ctgaaacttt tgcgttgtac
     1081 cactcggtgg cgattgcggc aaaagttctt ccttcggaaa ggtttttctc aagattttca
     1141 gctttcttga cttcaccggg atctttacca tcggcatgta ttttcttggc ttcatcccga
     1201 cgtttgcgag cttcagccaa tgtaatagtt ggataggtgc caaatgatag tttcttttcc
     1261 ttccccgcat agtggtactt cattcgccag tactttgagc cgttaggatt gacctgaagg
     1321 tagagcccac caccatcggc aatcttataa attttatctt tgggtttggc tgtttcaact
     1381 tgtcgagctg tcagtggcat ctgggggcct cgcaatagat tgaccaagat gtgcccctaa
     1441 ataggccccc aaatgtgact tgatttacgt tcccctgagg agaattaagg cgactatttg
     1501 agtaacataa gtaagggtat gtgctgatta taaaggcttt ttgtggggta tggtagattt
     1561 caggagaggt gtgcatggtg tcccctgcag acatctactt agagtggcag gggattgatt
     1621 agaatggtat tttttagatg tgagaaatat tttacccgct attttaccca ttagcgcggc
     1681 ttaagagctt atttttgaat tcacaatggt cacgatataa ccatcttgct cgaccgtgga
     1741 taactttggc tttaggcagg tcgccggact taatccggtc gtagatgaag gttttaccaa
     1801 agccagtatc agccatgatg aatttcaaat caaccagtga atcaggttgt agttcgtgtt
     1861 gcatgagtgc tatctccgaa tagggaatcg aacctgcaaa tcaggcaata aaaaaccgcc
     1921 atcaggcggc ttggtgttct ttcagttctt caattcgaat attggttacg tctgcatgtg
     1981 ctatctgcgc ccatatcatc cagtggtcat agcagtcgtt gatgtcctct gcttcgataa
     2041 ctctgttgaa tggttctcca ttcaattcac ctgtgactcg gaagtgcatt tgtgatttcc
     2101 ccaaaagatg cttgagtgcg cttcttattc gattcgcaca cctggtattt cgccttttga
     2161 aatggctaag tcataaattt gcgcagcact atacccatct cgcatccatg aatctaaggc
     2221 gcgaatagcc tcgctacgct ttttatcttc tctttcattt ttgatatcaa cgaggacatc
     2281 aacgcaatta aggcatatgt ggattttgtc cttacattcg atcatggcag ctttaccatg
     2341 atttccgcca cacagtgagc ataaatcttc agggtctggc tggtatttct gtaacgttaa
     2401 agggttgaat gttgaacagg ccataatcat ctccataaaa caaaactcgc cgtagcgagt
     2461 tcagataaaa gaaatccccg tcagtgcgag gattgttatt gtcttttctt cgtgcatttg
     2521 tcgcactttc ggcaccatcc agataggcac atccgtccgc aattaacaca tataggccac
     2581 atcatttttc ctcttttggt ttatgaatct gaatggtcat gccgctttga gtggtgacta
     2641 caatgacata accaggctga agactgttaa gattgaatgc ttcgtaaaac gaatccaagg
     2701 ccagcgcttt tttattcttt cggttccacc aacgccatcc cttgctacag gctacactga
     2761 caatccactg cccactcctg taagccatat aaaaccagat gagcaaaacc tgaaggaatg
     2821 ctatccagtc aataatcgta tatttcgcga aggagtccat cacttcacct cctgctgcgg
     2881 tgctgctggc atttcacgcc agtgcgtaac tgagtgcgga tccggatatt cggtgccatc
     2941 atcccagcga ttgccattcc acattgcaga ccacatctca ccgtcttcat acatgaccat
     3001 taccggaatt aacttatccg gcattcgctc actacagcat atccaaccat ccggagttac
     3061 cggagagttg cccgatagcg cattacgcaa tcgctccagc ttcacgtatt cctgaaccct
     3121 gtttccgtcg catgcctgaa gccattgcgc agccttttgc gcatcagtgt gaaaggcaca
     3181 agtgcgaccg tcatcaaatt gcatttcgta gaggtcagca acttgttcaa actgcgtatg
     3241 ttgtgacttg taagtttggc ttacaggttc ggcaccatga agcatggcgg cgcggagttt
     3301 ctgtatctcc cgtgccatta ttgcgtcctg acgaggagtg aacatatcac ctgcaataat
     3361 tctgtttagt tcctcgtcag tgaactcata atcatcagga acattgtgaa cctcagcata
     3421 aagaggcttt tttgtagaaa tggtcatcgt aaaacatcct ccgcacttac cagtccgttt
     3481 cgcaaaagat aatccatcgc cctatcaggt aatttgcaat caggttttgc ttttttcagt
     3541 tggctgacca attgtttaac cagcattgtt aattcgatca cctggtagcg cggcaatggt
     3601 gaattatcgg atttgccctg actgtcatca ctgcatgaat gcccttccag ccaggccaat
     3661 gcttgtcgca tgaaatacgc aatatgtttg ccgtggtaat cgtcttcatc gatgtgaaaa
     3721 gcgatactgc ggatgtattc tattgcattt tcaatggcct ccaacgctat cggcgctggc
     3781 ggtgcggtat aaaacttcgt ccccagcggc aacaacttca tcgctttttc tcccttaatg
     3841 atgcgataag ttgatttccc gccaaggtct accgttccat ccataacaag gccgagtcgc
     3901 ttctctgaaa cctcaccaat aggctctgct tccagcgatg ccagagcaat tcgtgccagt
     3961 tcttccgctt cttctgctgg cagtacaacg ttgctaccag gtccgtatgt ttcgcgccac
     4021 tgcttgattg tcagtagtcg ctctttggta atagtggtca tgggttatcc tcttagcgcc
     4081 acaacgcgca cttctgggtc aaaagatggt gcattctcat ctgtaacatc agcaacatga
     4141 cctacaaagt cgttatactc accctctgat tggtcatagc cctgccagac cacgacagcc
     4201 tcaggaggca tttttctgag cttgctgatt aactgcctaa ccgtcagcga catctcactc
     4261 tcctttgatg cgaatgccag tagcgcggat agatttccat tgtttccaca ttcggttgaa
     4321 gtctgtgcct gataaggttg cgttacggta tccagtttcg gtacgcaatc cttcaagcgt
     4381 cgatttggag atacaaattg aatgcattac ccatgattca aacaaatcac gctcccgggc
     4441 ttcgcaactt tgcttactca tctcttcgat acgttcagcc atcgcagcac actcttcaaa
     4501 gttgcttaat gcttttcgct cccattcggc gcattgtttt ccaagctctg caatcagctt
     4561 gtctttgcct tccagctcaa cacgcagttt ccctaccgtt agcgcaatat cctcgttctc
     4621 ctgatcgcgg cgtttgatgt attgctggtt tctttccagt tcatccagta atgccagcac
     4681 agtagccgga ttggctgcgg cgatgaattc agcattggcc tgctgttcca tttggaaatc
     4741 ttcatcgaaa ccgctttctg gatgtgctcc ttcaattctg caaatgggaa gatatccaac
     4801 aacttcacga tgaattaacg catcatcaca atcaaatcgg ctctctccat attcgagcga
     4861 ccatacacca cacgttgctt tttctgcctt ggcacgcagt gcctgatagt caatcaggct
     4921 cactggttgc ctcctttgcg aatctgttcc gcccattctt caagggattt ctccgcatat
     4981 tcaccagaca ggccatcaat cggatgcggt tcattagcca actcttcttt cgctgacaga
     5041 atcatgcgcg taacatcgaa aacttcacgt aaagacttat tgataaatcc gtggttgaaa
     5101 gcagcagcaa gacggcttgc ggcatagtta ataccctcgt tgcgtgcttc cgcacgtact
     5161 tcagccagga aagcatcggt ggctggggtt ttgatttcgt taagcgcatc actgaataaa
     5221 ccacgctcca tacctagctc tgcttcgtaa tcggcatcga atgcagcgtc tttgcagaac
     5281 ttcttcatcc ccgcactctc cgctgccagc gccgcgcact tggcctcaag gttatcaatc
     5341 gtgattccag cagaacgaca ctcccgcaac gccgtttcca gttttgattc aagttcaccg
     5401 aacttacgga caagatattc agcgtttgtt tcgttaacct ttagatctcg tgggatgcat
     5461 ttacctttca gaaaaccatc catctcaatt agtgacattt gtttcatttt ttcccactcc
     5521 gccacatagc attcagatat ttgttgtcat taacagaacc gaaactcttt ctcttaagca
     5581 attactctct cgatggcatt ggcttt
//
LOCUS       DAZGOB010000210         5588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGOB010000210 DAZGOB010000000
VERSION     DAZGOB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.210"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..358)
                     /locus_tag="ACHLIF_004812"
     CDS             complement(<1..358)
                     /locus_tag="ACHLIF_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO9608654.1"
                     /translation="MTLTLLTAYNVPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLI"
     gene            complement(625..1290)
                     /locus_tag="ACHLIF_004813"
     CDS             complement(625..1290)
                     /locus_tag="ACHLIF_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO9608655.1"
                     /translation="MTILSLENPVILLLENMLEALSTAPDSINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(1431..2072)
                     /locus_tag="ACHLIF_004814"
     CDS             complement(1431..2072)
                     /locus_tag="ACHLIF_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO9608656.1"
                     /translation="MCLSELQAGLNEDQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNIRELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAPPLPESRIALLLHLVSDDGALT"
     gene            2767..3798
                     /gene="repA"
                     /locus_tag="ACHLIF_004815"
     CDS             2767..3798
                     /gene="repA"
                     /locus_tag="ACHLIF_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO9608657.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATSFLVEPERK
                     RTRRRRGEHSTKPKCENPSWYRPARYKELSGQLGHAYNRLVKKAPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAKEISPKD
                     SKGNVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            complement(4758..>5588)
                     /locus_tag="ACHLIF_004816"
     CDS             complement(4758..>5588)
                     /locus_tag="ACHLIF_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608658.1"
                     /translation="VVSLNSNLKNEVVLSGVMTGTSKVFHLNKSGDLLLTTSKTHGGG
                     VIVIFKDFINNWWKYNLTLITVPIDDKLSDNRINITPMGITIHEIVNGDDKLFFYPTP
                     LKNGGFILHNPLYTNSFPSSYSHELFDLVEAYRNPTYSYLQNSIINRYIHTVSEHNGK
                     DGVAQMSLPIYAYSTCRFWPKESVAVGDTINLSNYEHIEFSFNSFSKDVCREQNSDIY
                     KIFFKIVALNNSIVVSENMFTQKNFLEHNTFFSVTGIDESLYEKHIIFITLKAIPPQK
                     "
BASE COUNT         1553 a         1315 c         1390 g         1330 t
ORIGIN      
        1 caatcagtct gccggtgcgg tgtacgagag gacatgtcag cagcaggcaa agtggtgcaa
       61 ggagaatgtt cggggcagga gcctgcagca gatgaataat aagaccctgc aggagaatgc
      121 cggatatacc aaaaaaacct gccagcatac agaggacgat cagcaggggg atccgtccga
      181 tgttcagcca gtccagcgcc tctcctgtca gcccctccgg taaatcaggt gtatccatag
      241 ctgaagagag ggagtgtccg cagagcaggg cgataagttc agcgatacca gtaagcagaa
      301 caaaagtcag ggctgccaga tagggtacgt tatacgcggt aagtaacgtc agtgtcattt
      361 ttcctcctgt ggcaggtcag tcaaacaaca ttatggcgtg aagcttccgc ttctttaatt
      421 gtcagaagaa gcatatcgtc agtcatcaga agacaagggg atgtctgcag gaggggatga
      481 taacaactga agcctcacgc cggaatgcgc tttatgacaa ccggagagga cggataataa
      541 aaaatgggac atccacatca gcaaaccgcg atttgcgaaa aaaagtcagc ggagagtggc
      601 tgccgttgtg gctgtcccgg tatttcagtg taacaggtga ttgctgtgat ctatgcccca
      661 ttcggctttc ggtaaacctg aatgccccac atgaagaatg cgtgacagca tgtttctgcc
      721 gctgccgttc aggctgcatt cgcgtccgtg cgcgagtata aaaccttcat cccggaatat
      781 ctcttccact tctgaagatt cgagggatgc cggatgaatt aatgaaaatg ccaccggtct
      841 gagcattctg cccgtggtgg aaaaggtgtc tctggtactg ttcagctgca ggatatgacg
      901 tttacctgtt tgcgcacggg aaagtgtctc actcagcagc tggtcgttcc atgcgcacgt
      961 gccaatctgc catcccagac ttctgcacgc ggaaagagcc gagcggaagc ggtacaggtc
     1021 gtataatgct gcgttattgc cactttccag cagtgatttc agcgtatttg ctaccggtat
     1081 gtccgtattg gtggccagca gttttcgcag agtcgtaaac tcttctgcca ttcctctgaa
     1141 tgcgctcatc tgacgcgcag attcaagcca gttcagcagg taacgccggc gacgccgttc
     1201 gttgttgata ctgtccgggg cggtggagag tgcttcgagc atattctcca gtaacagaat
     1261 gacagggttt tccagggata gaatggtcat aaaggcgtcc tgaaaaaatg cccgacagcc
     1321 gctctccgta caccgtaagg tgtcaccggt gccgggcatt gtggaaacag aaggaaaaaa
     1381 aatcccggcc ataaaggccg ggaaaagtcg tcaaattaag acagcaattt tcatgtcaac
     1441 gctccgtcat cagataccag gtgcaggagc agggcgatcc tcgactccgg cagaggcggt
     1501 gcatcaagcc ggaggaggta ctggtaacgc gcttttgtca gccagagggt ggaggcagca
     1561 tccagctcct ccctggctgc cggattgagc gcaggatccg ggtacacttt catcaaagac
     1621 tgaacgatat cctccctgac ggttttgatt tcaccggcca ttttcacgaa gttcagaaag
     1681 gcactgtgac ggcgaaggtg agtgaagctg tggcgttcgg ggtctgcttt caggaaaaag
     1741 taaccgggaa acacggcgtg tatatgtcga cggtaaccgc agactttatc cgtgcggcgg
     1801 acttttttca gtatcagggg tgtccaggga aggacgtgtt gttcggaaag ccaggaaaag
     1861 agatgttcgc ggtttttgcc ggcagggatg tactgagcca gataccagtt gcgttgatgt
     1921 aattctctga tattcaaaat ccggaacgct ccgcgaaaat cgatccccgc agaggggctt
     1981 cgatgttgtt gtgaacggcg aatacataaa gcatgtgtcg gaaacagcgg ctgatcctcg
     2041 ttcagcccgg cctgcagttc tgacaggcac atgatttacg cactcttgta agccttgtgg
     2101 acggtttcga ggccggcgaa aaccgacaaa acctgcaaac aggattaatg gctggcaaac
     2161 atattacata aggatatatc tgtggcaaga gcgaagataa gtagttaaat agatcgttat
     2221 atttgataaa gcagcatatt acgctgggtt gtgtgaggtg tttggatgat tagatcgtaa
     2281 ttacaagatc gattcatcca aaaaacatga aaaacacagc ataaaagatc gtttgtggaa
     2341 ttgtctattt tttgacacag ttcaaaaaaa cgacgttttt taggtggtta atgagaggcc
     2401 tggtaaatca gagaaatcgt gttggctttt caaagcggtg gaaaaggggt atattgcgga
     2461 tcgttatgca gtggcttttg ggatcgtcag gatccgggaa gtcagaaaat ggcaggatgc
     2521 gccataaggc attcaggacg tatggcagaa acgacggcag tttgccggtg ccggaaggct
     2581 gaaaaaagtt tcagaaggcc ataaaaggaa aacccccata atcttctcat cgaacttggc
     2641 ggaacgttag aagattatgg gggcctcaca gaatacggga taagtatata tgaaaccgtg
     2701 tcagagattc aacccttcgc agtgtattaa cgcacggcat tctgtttccg ccataagcga
     2761 cagcttgtgg caggtctgaa gaatactcca tataacgcag tacactggag tcagttagca
     2821 cccgaagagc agatccgttt ctgggaagac tatgaagcgg gaagggcgac ctctttcctg
     2881 gttgaaccgg aaaggaagcg caccaggcgc cgtcgtggcg aacactccac taaacccaaa
     2941 tgcgaaaatc cgtcctggta tcgtcctgcg cgctataagg agctgagcgg gcagctcggg
     3001 cacgcctaca accgtctggt gaaaaaggcc ccggtgaccg gcgagcagag cctgcgtatg
     3061 cgtatgtcgc ggcatccttt ttacgtgcag aaacggacgt tcgtcggtcg caaatatgct
     3121 ttccggccgg aaaaacaacg cctcctcgat gccatctggc cggttctggt cagcttcagt
     3181 gatgccggca cacacactgt ggggatgagt gtcactcgcc tggctaaaga aatcagtccg
     3241 aaagacagta agggaaatgt tattccggaa ctggaagtga cggtctcccg tctttcccgt
     3301 ctgctggccg aacaggtgcg ttttggtgtg ctgggtgttt cagaggaaac gatgtgggac
     3361 cgtgaaaccc gccagcgtct gccgcgctat gtctggataa caccggctgg ctggcagatg
     3421 ctgggcgtcg acatggtaaa acttcacgaa cagcagcaga aacgactgcg tgaaagtgaa
     3481 atccgccagc agctcattcg ggaaggtgtt ctgcgtgagg atgaagatat ctccgtacat
     3541 gcggccagaa aacgctggta tctgcagcgc agtcaggatg cgctgaaaca ccgtcgtgcg
     3601 aaagcagcag ccagtaagcg cgccagacgc ctgaagaaac tgcctgccga ccagcagatt
     3661 catgagatgg cagagtatct caggaagcgt ctgccgccgg atgaagccta tttttgttcc
     3721 gatgaccatc tgaagcgact ggccatcagg gagttgcgtc agcttgaact gacgctggct
     3781 gccccgccac cgcactagac agcaccattc cctcagcact gaatcatcgc cagcccctcc
     3841 ggggctttcg gcgcaggtat cgctcagccc aaaatccgca gtaatcacct taaatcccct
     3901 cagaggggca tatttgctca taaaaccacg catcagtcat cagaacgtgg ccacgttgtt
     3961 tcagttatcc acataaatcc gcaaacaaag aactttaaga agctgcaaac ctgaaacagc
     4021 aaacctgcaa tatagtctta accccattat ttaatcccct gcgttgcttc gccgcagcga
     4081 aagtctttat ctctgaaacc actgtgaaca aacacaaaga ggccttcgct tgcagcggcc
     4141 aaggccgcgc cgctcagaat ctaaaagcac ctcccacgct gacgcgcggg ccccgaacct
     4201 caccgttctg gaaccacaac aaaaaaacat caggaataaa aacaccacac aaacgcagca
     4261 ccgtgcccac ccctcataac tgaaaagcgc cgccgccccg gcccaaaggg ccggaacaga
     4321 gtcgctttta attatgaatg ttgtaactaa acatcatcat cgctttaaga ccccatttgt
     4381 ctagcagggc gatggcccac tgcgtgaacc atcaccctgc acacagggta ttttatgtgc
     4441 aattgtcaac cactgaatcg gaaccccaga ggggccccca cgattcagaa gttgacaatt
     4501 taagcacagg gactacatga gaaaaatcgt ggttcgtctg tcatacacgc gcgtaagcgg
     4561 cactgatagc cctcttacgc ggaaatacgc ccataagcgc taacttaagg gttgaaccga
     4621 cgccacggtg tctcgttaag acttcaattg cgttttgtag gcagtcaggg atactgtccc
     4681 atgaatggcc acctgtaagc tccagataac catttttgtt attctccaca acgaattccg
     4741 cataccggaa atcagcgcta cttctgggga ggaatggcct taagagtgat aaagattata
     4801 tgcttttcat agagagactc atctatgccg gtaacactga aaaatgtatt gtgttccaag
     4861 aagttttttt gagtgaacat attttcgctc acaacaatcg aattattcaa agccacaatt
     4921 ttaaaaaata ttttatatat atcactattt tgttcacgac agacatcttt actaaaagaa
     4981 ttaaagctaa attcaatatg ttcataatta gataaattta ttgtatctcc aacagctaca
     5041 ctttcttttg gccaaaaacg acatgttgag tatgcatata taggtaatga catctgagct
     5101 acaccgtctt ttccattatg ctcagaaaca gtatgtatat atctattgat aatactattc
     5161 tgtaaatatg aatacgttgg atttctatat gcctcaacca aatcaaataa ttcatgtgaa
     5221 tatgaagaag gaaatgaatt agtgtaaagt ggattatgta agataaaacc tccatttttt
     5281 aatggagtag gataaaaaaa caacttatca tccccattga ctatttcatg tatcgttata
     5341 cccattggtg ttatatttat cctgttatct gacaatttgt catcaatagg aactgttatc
     5401 aatgtaaggt tatatttcca ccagttattt ataaaatctt taaaaataac tataaccccg
     5461 cccccatgag tttttgaagt ggttaacaat aagtcgcctg atttatttag atgaaaaacc
     5521 tttgatgttc ctgtcattac tccagacaaa acaacctcat ttttgaggtt gctatttaaa
     5581 ctaacgac
//
LOCUS       DAZGOB010000211         5314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGOB010000211 DAZGOB010000000
VERSION     DAZGOB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.211"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            264..2969
                     /gene="malT"
                     /locus_tag="ACHLIF_004817"
     CDS             264..2969
                     /gene="malT"
                     /locus_tag="ACHLIF_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO9608659.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3012..4028)
                     /gene="rtcA"
                     /locus_tag="ACHLIF_004818"
     CDS             complement(3012..4028)
                     /gene="rtcA"
                     /locus_tag="ACHLIF_004818"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO9608660.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4032..5258)
                     /gene="rtcB"
                     /locus_tag="ACHLIF_004819"
     CDS             complement(4032..5258)
                     /gene="rtcB"
                     /locus_tag="ACHLIF_004819"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO9608661.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT         1251 a         1497 c         1338 g         1228 t
ORIGIN      
        1 atggcacttt catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa
       61 gggaaaccag tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg
      121 gaattgtgac acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag
      181 gtttctggcc gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat
      241 tttccacagt gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac
      301 tcgaccatac cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc
      361 ggctggcgct gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg
      421 cggcaggcaa aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc
      481 gtttcgccag ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat
      541 gtgagacgat ggcgcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt
      601 tcattgagct ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga
      661 tcactaatcc agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc
      721 tcaccctggt ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc
      781 gtgatcaact gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc
      841 agttttttga ttgccgtctg tcatcgccga ttgaagctgc agaaagcagt cggatttgcg
      901 atgacgtttc cggttgggcg acggcactac agctaatcgc cctctccgcc cggcagaata
      961 cccactcagc ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg
     1021 attatctggt cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga
     1081 aaagcgccat tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa
     1141 acgggcaaat gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg
     1201 ataccggcga gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc
     1261 agtgggaact ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg
     1321 cccagggatt tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc
     1381 tgcgcgatat tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc
     1441 tggaagagtc gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt
     1501 tattgcaggc gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag
     1561 cccgtgctga acatgaaatc aaggacatca gagaaggcac catgcacgca gaatttaacg
     1621 ctctgcgcgc ccaggtggcg attaacgatg gtaatccgga tgaagcggaa cggctggcaa
     1681 aactggcact ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg
     1741 tgctgggtga agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctaatgcagc
     1801 aaaccgaaca gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaatcc
     1861 agcaaagtga aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acacaggaaa
     1921 aagcgttcca gctgatcaac gagcagcatc tggaacagct gccgatgcat gagtttctgg
     1981 tgcgcatccg agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg
     2041 cgcgtagcgg gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg
     2101 caatgttgat tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagctga
     2161 accgtctgga aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca
     2221 acaaagtccg ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt
     2281 tgcgtcatac ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca
     2341 acattgcccg cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag
     2401 aactcaatga aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc
     2461 tgcttaatca actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg
     2521 acgcattaaa actggcgaat cgcaccggat ttatcagcca ttttgtcatc gaaggcgaag
     2581 cgatggcgca acaactgcgc cagctgattc aactcaatac gctgccggaa ctggaacagc
     2641 atcgcgcaca gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt
     2701 tcgatgagaa tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctgatccgca
     2761 ccagcccgtt gacccaacgt gaatggcagg tcctggggct gatttactcc ggttacagca
     2821 atgaacaaat tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca
     2881 atctgtacca aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac
     2941 tgctgaagat gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt
     3001 acatctttac atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac
     3061 cgcaccggca agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg
     3121 gcgaccttaa attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga
     3181 tattccccca ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg
     3241 gctaccacct cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt
     3301 tcactttcga cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga
     3361 atattctgtt catgcaggga aaaactcccc gccagtgtag cgatttcacg ctcagcaaca
     3421 tggcgcggca caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg
     3481 ccaagttgca aggtgttaaa cgatgccacg ggcgagactt ccgttgccac cacgccgcct
     3541 ccggcaggat aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc
     3601 agcagcggct ccagcacccg gcggataaaa tccgcaggcg gggccgacgg gttatcggtg
     3661 cctccgctca cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt
     3721 tgcagcacca gcgtacagct tccggcacta ccgatagcaa agcggtactc gccgccgcgc
     3781 acggtgccag gccggaagac cagacgctgc gaccccagct ccgcgccttc caccgttgcc
     3841 ctacaaattt ccgcagccgc ttttaccgcg gtcagatgct ggcgcaacag ccccggtttc
     3901 gcccgcccgg cacgaatgct ggtgatggta aatggctggc cggttatcat cgacaggctc
     3961 agcgccgagc gcaggatctg cccgccacct tcgccctgtg cgccatccag cgcaatcatc
     4021 cttttcatca tttatccttt tacgcacacc acctgacgca gggtatagat aacttccacc
     4081 agatcgcttt gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg
     4141 atcacttcgg catctttacg gcattccaca tgcgcggtgg cacgaatttg atcttccacg
     4201 ctgaacagtt ttttcgcttt agtacggctc attacccgcc cggcaccgtg gctgcacgaa
     4261 cagaacgact cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg
     4321 ggaataattc catattgacc agcacgcgca gacaccgcgc ctttacgcgt cacgtagatc
     4381 tcttcaccaa agtgctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc
     4441 gccagcgttt gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccacg
     4501 ttttccatca tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt
     4561 aggtaatcat caaagtattc cgtaccttcc ataaagtacg ccaggtcgcg cgatggcaac
     4621 gtttcaagct ggtcctgcat ctctttttgt gccaaatcga taaagtaagt cccgatggcg
     4681 ttaccaattc cgcgtgaacc ggagtgcagc ataatccaca cctggtccga ctcatcaagg
     4741 cagatttcaa taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg
     4801 ttaaggaaac gcggatattt ttgcgttaac cactgataac cggcttcaag ctcagcccat
     4861 ttcgcatcga cgttaacagg cgggttttcc catgcgcctt tatcacgttt acaacggcca
     4921 gtggtacgtc cgtgcggcac cgcagtttca atcgcctgac gcagctctgc caggttttca
     4981 ggcaggtctg ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg
     5041 cccaccgccg ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct
     5101 ttacccaggt gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc
     5161 gtattaataa gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt
     5221 accggggcat tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt
     5281 ttgctgtcgt gataattgca accgtcgtgc caga
//
LOCUS       DAZGOB010000212         5250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGOB010000212 DAZGOB010000000
VERSION     DAZGOB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.212"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..469
                     /locus_tag="ACHLIF_004820"
     CDS             50..469
                     /locus_tag="ACHLIF_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO9608662.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            466..1026
                     /locus_tag="ACHLIF_004821"
     CDS             466..1026
                     /locus_tag="ACHLIF_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO9608663.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDTSSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            1027..1272
                     /locus_tag="ACHLIF_004822"
     CDS             1027..1272
                     /locus_tag="ACHLIF_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO9608664.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            1269..2771
                     /locus_tag="ACHLIF_004823"
     CDS             1269..2771
                     /locus_tag="ACHLIF_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO9608665.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATELWGRGSMIALMVAEFIAINPDAELYAIAQGNGT
                     GQATAAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPF
                     TASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYHDGEATPEGIQVAIAYPKQK
                     AANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWMAHTG
                     TFGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            2780..3145
                     /locus_tag="ACHLIF_004824"
     CDS             2780..3145
                     /locus_tag="ACHLIF_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO9608666.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            3160..3636
                     /locus_tag="ACHLIF_004825"
     CDS             3160..3636
                     /locus_tag="ACHLIF_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO9608667.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            3763..>5250
                     /locus_tag="ACHLIF_004826"
     CDS             3763..>5250
                     /locus_tag="ACHLIF_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9608668.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRG"
BASE COUNT         1180 a         1366 c         1613 g         1091 t
ORIGIN      
        1 aggatgctcc ccggaaaaag accggtaaca aagcggaaca ggcccgtgca tgaattacgc
       61 cactgaaacc gatatgcggg cgcgttaccg cgaggatttg ctcaggccgt tactggctgt
      121 gccccgttcg gatgagccgg acacgcgcaa gctgaaccgg gcgctgacag atgcgtcagc
      181 ccttatcgac agctatctgt ccgcccgtta cacgttgccg ctggaggtta ttccggccgt
      241 tcttgttcag cactgttgcg cgattgcctt ttattacctg tgcgatcagc gagcctccga
      301 tcaggcgcgt gaccgttacc gtgaagcgct ggcctggctg aaagatgtca tgaacggcaa
      361 tgtgccggtc ggtgtggata cgaacggtgc ggcccctgaa tccggggatt taccacaggt
      421 tcagtctgat gcagcggtat tcgggcgcaa ccagaagggc ttcatatgat tacggaaacc
      481 gaacaggcat acatcgcccg tatccgtgag tatttcggga atgaactggt gtctgttgac
      541 acgcatcccg gcgactggag cgacagcgta ctgcgcacca tgctgattaa cgccccggcg
      601 atctacgttg cctggctggg tgccggtgaa ggccgtaccc gtggtcgcct ggtcagtcac
      661 tgggtgttct acgtcatcgg cgacatgctc aacgggcgtg aggccagccg tcccggactg
      721 tatcagattg tggcccggct gattgccgtg cttaacggct tcagaacaga aaaaacctca
      781 ccgctttact ttgaaaaggc ggtcaacggt tacaccgaaa cccaggcgga cagtggtgcg
      841 gtgatgtatg cgctttattt ctcctgcgag gaaatgatcg caccgctgac cgataccagc
      901 tcgctggacg acttcctgcg tcattacgaa acctttgtgg agccgcccgg tacgccgccg
      961 tttgaggcac atattgattt gccggggaac acgacaacgg cggtcgaacc gccggaggaa
     1021 ctgtaaatga aaaccatctt tatcaaaccc gcgccgggac gcctgattcg tgatcccgac
     1081 acaatgcgcc cgctggcaca ggagggggag gaaaagccct ttacgccgtt ctggtgtcgg
     1141 cgtcttgatg acggcgatgt cattcaggct gaaaaagccg ctgaagaggc tccggctgta
     1201 tctgcggatg caacgactgc gacagaaaaa ccggtcgccc aaccggcatc cgataaggag
     1261 aaaccgcaat gattaatttt gacgggatcg gtaatgaccg ccgtataccg ctgattgagg
     1321 ttgagtttaa taactccatg gctgtcaccg gcactccggc acagcgtcag tgtgtgctgc
     1381 tgtttggtca ggcgagaatg aaagagaatg ccgtggacgg tgccggtgtt ctggatgttc
     1441 cggtcaggat cacccgtgcg tctcaggcaa cagaactctg ggggcgcggc tccatgattg
     1501 ccctgatggt ggcggaattt atcgccatca atcctgatgc tgaactgtat gccattgctc
     1561 aggggaacgg caccggacag gcaacggctg cggccatgaa tctgaccgga acggtcaccc
     1621 gtgacgggat tgtttatgcc tgcgtggggg ggcggcgtta tacactcccg gcaccaaaag
     1681 gtaagaaagg caaagaactg accgacgagc tggccgcgct gatcaatgcc gatcctgacg
     1741 cgcccttcac ggcctcctct ggtgcaggaa gtggtgataa tggcgcaggt ctgaaagggt
     1801 ctctggggat caccgcccgc tttaccggtg aatgctccgt tcatgatgtg cgcctgaact
     1861 atcacgacgg ggaagcaacc ccggaaggaa tacaggtcgc tatagcctat ccgaaacaaa
     1921 aagcggctaa cccggatatc acccgaagcg tggcgggtat gggcgatcgc cagtacaact
     1981 atgtcgtcat gccctataag gatgatgcaa acctgaaaat catcagtgat gagcttctga
     2041 aacgctgggg accggccaaa atgtctgacg gtgtgctgtg gatggcgcat accggcacgt
     2101 ttggtgaggt gcaggcattc ggggcaaaac gcaatgattt tctggcgacc tgcacattca
     2161 ttcccaaagc ccctgaaccg gattacctct gggcagcggc ggtctgcgcc acctgtgcgc
     2221 cgtctctggg ggcagatcct gcgcgtccgc ttcagacgct ggcgcttccg tcacgcatgg
     2281 ccccggcccc ggccgatcgt ctgacgcgtg aagagcgtaa cagtctgctg tggggaggga
     2341 ttgccacggt cagtgtggct gccggtgatg tggttcagat cgaacgtcag gtgaccatgt
     2401 atcgccagaa cgtgtacggc gaaagtgatc ccagctatct ggatgtggaa accatctaca
     2461 ccctctcctg gctgcgttat tccctgcgta cttttatcac acaacgtttt ccgcgccata
     2521 aactggctga tgacgggacg cccgttcgtg ccggtcagaa tatcgtgacg ccggagatca
     2581 tgaagcttca gttaatttcg ctgggtgaag agtgggtgga actcgggctg gtcgaaaatc
     2641 tggacacctt taaaaagaat ctgcttgtgg agcgtaatgc gtcagatcga aaccgtcttg
     2701 acgtgttatg cacgccggat ctggttaacc agttccgctt cctggcagca cagatccgtt
     2761 tcattttgtg aggtaaggca tgagcggaaa acagtatcag ggcacggcca ctatccgtgt
     2821 gaacggtcag gaatacgaga cgcttgaggg ggctacattt tctccgtcag gttttgaacg
     2881 tgaagtggtg aaaggcgcga aagtctatgg ttaccgccag aaaccacgtg aagcgacact
     2941 ggactgcaaa tttccggcag gcggagaagg ttcaccagcc gccgatgaaa tcaacaactg
     3001 gactgctgtc actatcgagt ttgtggcgga taccggcgaa gtccacatga tgacgaaggc
     3061 ctggagcagt gaaccggcct cgcttgacgg tggcggggag atctccgtga agttcgccag
     3121 cgccaccagt acccgtgttc agtgatcagg aaaaataaaa tgaccacacg taagaaaaaa
     3181 acggcagttt ccgaggcagc cgtgatggag gcaatccggg aagcacttga gggggctgat
     3241 ccacgcactg ccgggctgac agagcaactg gcaaagggat atgtggatct gcttgatggt
     3301 ctgccgtttg gtgagacccg tgaatatcgt gtcacgttcc gggaactgac agcgaaagac
     3361 agtattgacg cagaagcaga agccgagcgc gtggtggaga caaacaatgg cccgatgctg
     3421 atagcgtctc cgtcgttacg cggtgttgca ctgctgcgcc gtcagattgc cgcagtggga
     3481 gacattgaag gtccgttgtc accccgtcag attggacagt taagcgagcg cgatctctcc
     3541 cgcctgatgg cagcggtcag cctgctggat accgcgcttg ccggaaagct ggcagctgac
     3601 cgggggcgat caggcgcagt gtcgggatca gattgaagaa gcggcaatta tcctggggat
     3661 ggtgacaaaa agcggtccgg aatgggcgct taacctcccc ctgtcgcaac tttaccggca
     3721 ctgccgacag acagaaaaaa tcatcagaac gaagcagtaa gtatggcccg aaatctcaga
     3781 gcatctctga tagttgattt gctcggcaat atctcagcca aatcccgcca gtggtcacag
     3841 gagctggggg cattctcacg ctccggccag gcggggctgg gtggcctggg aaatgctgcc
     3901 cgccgtgccg ggcaggaaac agatgtactc ggaagcagga tgcagcgcac gctggccggg
     3961 gtgcggggca gtatccgcca tgtcacttct gattttgacc gtcttcaggg cagtattacc
     4021 ggcaccatcg ggcggatcag caatctttac ggaatgctgg ccggtggtgc tgctgtatat
     4081 ggctttaacc ggggatttat tcgcccggca gcggagatgg aaacctatat gattcgcctg
     4141 aactcccttt acaaagggga tcgggcgaaa actgatgatg tccgcaggtg ggcaatacag
     4201 aatgcgaagg aaaccacctg ggggctggct ggcgtcatgc aggaatatac gtccagtctg
     4261 ggctttggta tgagtgacag ggaggcccga aattttgtca ccatgcttca ggatcagggt
     4321 gccgtgggcg gctggtcact gtccgatgca cagggggcat ccctgcaact gaaacagatg
     4381 tatgcaagag gcagtattca ggcagcggat gcaaatattc ttgccaccta cggcattaat
     4441 gcctaccgcg tgctggctga tcgtctgggt gtggaccaga aagtggtcag aaaacttggt
     4501 gaaaaagggc tgcttgggcc ggacagtatt cgcctgttat ttcagacgct ggcggaacag
     4561 gccaggggag cacaaaagga cgcaatgaat tcctggagtg gcctgacggc catgatgggt
     4621 gacgtctggg atcagtttgc ccgggatgtg atggacagtg ggccatttga aaaactgaag
     4681 ggtaatctca ggggatttct ttcatgggtt gacagtgcca aatctgatgg cagttaccat
     4741 tccctggcgg aaagtacggc atcagccatg aaccagggct ttgagtatgc ccgtgatgca
     4801 gtgacagggt tttatcaggc gatcagaaag gtacgcgaca cgcttcaggc tctgcgggat
     4861 gcgggctatg gtgatgcgct ggatcgcatt ggtcaggggg cacagaccgc cgcgaaatac
     4921 ctgctgtaca tgtatctcgc cacccgtgcc ctgaaaatgg cgcgggctgt cggaacgggg
     4981 attatacgtc cgggtgctgc attgctggga tacggattat cagcggcggc cttccttacc
     5041 tcacctttcc ggcgctctcc tccggccgga acaccaccag gcacatctca gggacgaggg
     5101 caacgtttta taaactttct taccggggta aatccggcag ccgttcagcc agtacttgta
     5161 acaaactggc ccacaggagg actgtccggc accggcgcga ccgatacatc ctcaggcgga
     5221 cgtcaggggc ggggccgcag gaaacgcgga
//
LOCUS       DAZGOB010000213         5219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGOB010000213 DAZGOB010000000
VERSION     DAZGOB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.213"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(57..443)
                     /gene="ridA"
                     /locus_tag="ACHLIF_004827"
     CDS             complement(57..443)
                     /gene="ridA"
                     /locus_tag="ACHLIF_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO9608669.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(516..977)
                     /gene="pyrI"
                     /locus_tag="ACHLIF_004828"
     CDS             complement(516..977)
                     /gene="pyrI"
                     /locus_tag="ACHLIF_004828"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO9608670.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(990..1925)
                     /gene="pyrB"
                     /locus_tag="ACHLIF_004829"
     CDS             complement(990..1925)
                     /gene="pyrB"
                     /locus_tag="ACHLIF_004829"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO9608671.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(1929..2063)
                     /gene="pyrL"
                     /locus_tag="ACHLIF_004830"
     CDS             complement(1929..2063)
                     /gene="pyrL"
                     /locus_tag="ACHLIF_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO9608672.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(2344..2739)
                     /locus_tag="ACHLIF_004831"
     CDS             complement(2344..2739)
                     /locus_tag="ACHLIF_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO9608673.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(2870..3583)
                     /gene="bdcA"
                     /locus_tag="ACHLIF_004832"
     CDS             complement(2870..3583)
                     /gene="bdcA"
                     /locus_tag="ACHLIF_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9608674.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3654..4247
                     /locus_tag="ACHLIF_004833"
     CDS             3654..4247
                     /locus_tag="ACHLIF_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO9608675.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            4392..4844
                     /gene="tabA"
                     /locus_tag="ACHLIF_004834"
     CDS             4392..4844
                     /gene="tabA"
                     /locus_tag="ACHLIF_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO9608676.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            4967..>5219
                     /locus_tag="ACHLIF_004835"
     CDS             4967..>5219
                     /locus_tag="ACHLIF_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608677.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAH"
BASE COUNT         1310 a         1239 c         1308 g         1362 t
ORIGIN      
        1 ttttgcgttt gtcatcagat ttaatccggg catgatagcc cggatttcca tcaagattag
       61 cgacgaacag cgatcgcttc gatctcaatt tttacgtctt tcggcagacg ggcaacttca
      121 acgcaagaac gtgccgggaa ggtggcattg tgttcggtga agaaggcttc gtaggtagca
      181 ttaacggttg cgaagtcgtt cagatctttc acaaacacgg tggttttaac gatgtcgccc
      241 actttcaggc ctgcggcttc gacgatcgct tttacgttat ccagcgactg acgtgcctgt
      301 gcagcgacgt ctgccggtac ttcgcccgtt ttcggattta ccgggatctg gccggaggtg
      361 atgatcatat tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt
      421 tccgtcgcga tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc
      481 attataggga gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg
      541 gaaaactctt tttcacagta tttgcatttg agcgcgatgt cattggcgcg ttttcgcacg
      601 gcaaagctgg aagaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc
      661 acattgtcga tgcgctccgg cagactgggg cgcgatttac ccaccacttc atagttgtcg
      721 atacggttaa ccgtggcttg cggcgcatac aatgccagtt gatctacctg atcttcactc
      781 aaaaaggtat tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaggttc
      841 agaccaatgg tgatgcgctg atccgtttcg gtcagcttga acagactcaa cagcttaaaa
      901 ccgatctggg cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat
      961 ttattatcgt gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt
     1021 gccagtaacg cctggcgagc gaaaatcccg ttgcctgcct gctggaagta ccaggcgtgt
     1081 ggcgttttat caacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc
     1141 atattggctt tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg
     1201 gcgtactcgg acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact
     1261 tccgccatca cttcttcaat agagctgtgc agactccatg cgatcccttt ttcatcgagc
     1321 atatccagaa tgtattgcgg cattgccagc gcgtccggcg cgatgaagta aaaacggttg
     1381 ccatcgaact tcgctaacgc ctgagtcagg gagtgaacgg tgcggccata tttcaggtca
     1441 ccaaccattg cgacgtggag attgtccaga cgcccctggg tttcctgaat agtgaataag
     1501 tccagcaagg tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca
     1561 ttgccggaaa actcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc
     1621 gcatcgacgt aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ctttttaccc
     1681 agtgatgtat tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatagat
     1741 gtttcgaaag agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg
     1801 tgcttcaaca gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga
     1861 ttaaggtcat cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta
     1921 gccatctttt atctcctgac gcctgggcaa aaaaaagccc ctcgattgag gggctgggaa
     1981 tgggtgatca acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca
     2041 aaatgtcgaa cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata
     2101 gccagaagcg gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat
     2161 taaaagtcaa aatgtatgca tttttatttg ctttgattct ggaggagaac tgtcgttcgg
     2221 tatcaataat tgcagacatt tcctgctttt ttctttttca ctttcacgca atcagatatg
     2281 cattttattc cattcatccg acttataggg cggagtgtga aaacgaacgg ctaacactat
     2341 tgcttacagc tcagggatgc gcgctatcac tttaatttca aaatcaaagc ctgccagcca
     2401 ggtaacgccc accgccgtcc agtttggata aggaggggcg ctaaatattt catttttgac
     2461 cgtcatgatg tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat
     2521 atcgtcaaaa gtgcatcccg cagcagccag ggtcgcatgc aaattatcaa atgccagtct
     2581 gacttgttgc tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga
     2641 aacaaacagc aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga
     2701 atgtcggcca gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac
     2761 gacatgaaca gaagatttct ctttaccggg agccgctttt agcggacgac gtgagtaaac
     2821 aaaacccaga catcatggat aatggctggg cttaattgag cgtagtcggt tatgcgccaa
     2881 acgcgccatc aatggtatgc atcgcgccgg taacaaaact ggcttctggc cctgctaacc
     2941 atgcgaccat accagcgacc tcttccggtt gcccatgtct tttgatagcc atcaaactat
     3001 gcaacatatc gcgcattggc ccgttggcgg gattagcgtc ggtatcaatt ggccctggct
     3061 ggacgacgtt aatggtgatc ccacgcggtc caaaatcacg ggccagcccg cgcgccatgc
     3121 cttgcagggc agatttgctg gcggcataag cagccatgcc tgcaacaggc atacgatcgc
     3181 cattcacgga gccgatgatt aagatgcgcc cgccttcggg catctgccgg gcggcttcaa
     3241 cagaggcatg ataaggagca tgaatattga ttttgaaaag gcgatcaata tcgtcggcat
     3301 ttaattccag ggcatcgcca aagacgccaa tacctgcatt taccaccagg atatccaatg
     3361 cgccgctctt acgaacgaca tcaatgacag cgtctctgtc agcactatct gtaaatactg
     3421 ctgtcgctcc agtctcttgt gccagatgtt cagcggcatc tttcgacccc gcataggtga
     3481 atcgtacatt ggccccatca gtgacgaaac gacgtacgat agcggcaccg attccacgac
     3541 tgccaccgag gatgagaact gtcttacctg taaaagcgcc cataaggact ccttgattta
     3601 ttatgtaaca tgcattacaa aactgtttta actttctgtc aacaagtttt gtaatggtca
     3661 ctaaaaaaca atctcgcgtt ccaggtcgtc ccagacgttt cgctcctgag caggcagtct
     3721 ctgcggcaaa agtgcttttt caccaaaaag gtttcgatgc tgtcagtgtt gctgaagtta
     3781 ctgattatct tggtattaac cccccgagcc tctacgcagc ttttggcagt aaagttgggt
     3841 tatttagccg tgtactcaat gaatatgtcg gtacggaagc tattccgctt gccgatattc
     3901 ttcgtgatga tcgtccggta ggcgagtgcc tggttgaggt attaaaagaa gcggcgcgca
     3961 gatatagcca aaacggcggc tgcgctggct gtatggttct tgaaggtatt catagtcatg
     4021 atccacaagc gcgtgatatt gccgttcaat attatcacgc cgcagaaacg accatttatg
     4081 actatatcgc caggcggcat ccacaaagcg cacaatgtgt gactgatttt atgagtaccg
     4141 tgatgtcagg gctttctgcg aaggcacggg aggggcactc catcgaacaa ctctgtgcaa
     4201 cagctgcact ggcgggggaa gcgataaaaa ctattctcaa ggagtgatgc tggccttgac
     4261 ccgaaaggcg ggaacgcgcc tgccgataag ttgtgataag acaataattc acgcgttaag
     4321 gctagcggaa ttgatcatct tttcgtataa cggtggaaat gaaacattgt tttaattaag
     4381 gagtggaaaa gatgatcatc ggaaatattc ataatcttca gccgtggcta ccccaggagt
     4441 tacgccaggc gattgagcat atcaaagcgc acgttacggc agaaacgcca aagggtaagc
     4501 acgatatcga aggcaatcgt ctgttttatc ttatctcgga agatatgacc gagccgtacg
     4561 aagctcgccg tgcggagtat catgcccgct atctcgacat tcagattgtg ttaaaaggtc
     4621 aggaaggcat gaccttcagc acgcaacctg caggcacgcc ggataccgac tggttagctg
     4681 ataaagacat cgcatttttg ccggaaggcg ttgatgagaa aacagttatc cttaatgaag
     4741 gtgattttgt tgtgttttat ccgggggaag tgcataaacc gctgtgcgca gtgggcgcgc
     4801 cagcccaggt tcgcaaagca gtagtgaaga tgctgatggc gtgatgactt ttcgccgtaa
     4861 ataactccag gtttacggcg agtttgtgaa aagagcgttt tttgatattt ttttgtgagt
     4921 agaatttgta atgcttagac gttcttatta actcaaggag tccgtcatgg caaaaatatc
     4981 agatttgaat tgttatcaac acatggcatc agccgacaat gttaaaccaa aaaatgaagt
     5041 cttaaatacc tggtgtgtgg gaatgaatga ttttgctcgt aatgccgagg ggcaggatag
     5101 cagaaggaat attctatctc ctggagcatt tttggagttt ttggcaaaaa tatttaccct
     5161 gggttatgtg gattttagca aacgctccaa cgaagcgggt agaaatatga tggctcata
//
LOCUS       DAZGOB010000214         5214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGOB010000214 DAZGOB010000000
VERSION     DAZGOB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.214"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(96..842)
                     /gene="dsbG"
                     /locus_tag="ACHLIF_004836"
     CDS             complement(96..842)
                     /gene="dsbG"
                     /locus_tag="ACHLIF_004836"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO9608678.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            1214..1777
                     /gene="ahpC"
                     /locus_tag="ACHLIF_004837"
     CDS             1214..1777
                     /gene="ahpC"
                     /locus_tag="ACHLIF_004837"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO9608679.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1948..3513
                     /gene="ahpF"
                     /locus_tag="ACHLIF_004838"
     CDS             1948..3513
                     /gene="ahpF"
                     /locus_tag="ACHLIF_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO9608680.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3634..4062)
                     /gene="uspG"
                     /locus_tag="ACHLIF_004839"
     CDS             complement(3634..4062)
                     /gene="uspG"
                     /locus_tag="ACHLIF_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO9608681.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            4283..>5214
                     /locus_tag="ACHLIF_004840"
     CDS             4283..>5214
                     /locus_tag="ACHLIF_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO9608682.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFSYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYA"
BASE COUNT         1327 a         1265 c         1319 g         1303 t
ORIGIN      
        1 aaatagcata gccacttagg gcagagcgag caattcatgc ttttttagga tggttgaaaa
       61 gcagggatgt atttcaacca tccatgaatt atcttttatt tattccccat aatgatatta
      121 agcgttttct gatcgggcaa ccccacggcc tgttgcagcg tattttcctt actcatgtaa
      181 tagatcgctg gtgtgacatt tgcccccaga tcgtccatga gtttctcgtt ggcgcttaac
      241 actttcattt gttctgtact tacgtttgca ggcacgttta gcttaagctt gccaccagag
      301 gattcatatt cttgccaggt ttttgcggga tctttggagg caagaattgc cgctgctgtc
      361 gccgggcttt ctggcttgat aaccccaacc aacaatgttc ttaattgcac tttgccagaa
      421 tctacccacg ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa cggatcggcg
      481 aacacgtaga caatgaccgg cgcatctttt ttaccgtcga ggagccagtg ggattgttcc
      541 atccgttgcc acatttcgcg tccggctggt gcgtaaattt ctttttcgat aagtgtgtta
      601 ctcaggtttt cacctttctc gttgtacatg taaccagaga tagcgtgctt accatctgga
      661 gtcaggtaga tggtgacgcc catatcctga tactttccga gataaccttt cattcctccg
      721 ggggcatcga atgttttgat gattgtaatg ccctgttttt caatcgcttt tactggagca
      781 ggaagttcct ctgcgaaggc gattgcagga agcagagcca gtaaaagtat cttttttaac
      841 attaatttgt ccttttcagt cagtgcaaaa gtcgagtaaa aggcataacc tatcactgtc
      901 ataggtaaga gcttagatca ggtgattgcc ctttgtttat gagggtgttg taatccatgt
      961 cgttgttgca tttgtaaggg caacacctca gcctgcaggc aggcactgaa gataccaaag
     1021 ggtagttcag attacacggt cacctggaaa gggggccatt ttacttttta tcgccgctgg
     1081 cggtgcaaag ttcacaaagt tgtcttacga aggttgtaag gtaaaactta tcgatttgat
     1141 aatggaaacg cattagccga atcggcaaaa attggttacc ttacatctca tcgaaaacac
     1201 ggaggaagta tagatgtcct tgattaacac caaaattaaa ccttttaaaa accaggcatt
     1261 caaaaacggc gaattcatcg aaatcaccga aaaagatacc gaaggccgct ggagcgtctt
     1321 cttcttctac ccggctgact ttactttcgt atgcccgacc gaactgggtg acgttgctga
     1381 ccactacgaa gaactgcaga aactgggcgt agacgtatac gcagtatcta ccgatactca
     1441 cttcacccac aaagcatggc acagcagctc tgaaaccatc gctaaaatca aatatgcgat
     1501 gatcggcgac ccgactggcg ccctgacccg taacttcgac aacatgcgtg aagatgaagg
     1561 tctggctgac cgtgcgacct tcgttgttga cccgcagggt atcatccagg caatcgaagt
     1621 taccgctgaa ggcattggcc gtgacgcgtc tgacctgctg cgtaaaatca aagcagcaca
     1681 gtacgtagct tctcacccag gtgaagtttg cccggctaaa tggaaagaag gtgaagcaac
     1741 tctggctccg tctctggacc tggttggtaa aatctaaatt tccttcgtct ttcgcgctac
     1801 caactccggc gcgaaatact tcggaaattc acctaattct tcgggtgctg cggcacccga
     1861 ttttcttccc cgcaccatga tgcaagctgc atccaggtag ccgcagaggc cgcttgcatg
     1921 atgatgttta aagcccagga gataaacatg ctcgacacaa atatgaaaac tcaactcaag
     1981 gcttaccttg agaaattgac caagcctgtt gagttaattg ccacgctgga tgacagcgct
     2041 aaatcggcag aaatcaagga actgttggct gaaatcgcag aactgtcaga caaagtcacc
     2101 tttaaagaag ataacagctt gccggtgcgt aagccgtctt tcctgatcac caacccaggt
     2161 tccaaccagg ggccacgttt tgcaggctcc ccgctgggcc acgagttcac ctcgctggta
     2221 ctggcgttgc tatggaccgg tggtcatccg tcgaaagaag cgcagtctct gctggagcag
     2281 attcgccata ttgacggtga ttttgaattc gaaacctatt actcgctctc ttgccataac
     2341 tgcccggacg tggtgcaggc gctgaacctg atgagcgtac tgaacccgcg catcaagcac
     2401 actgcaattg acggcggcac cttccagaac gaaatcaccg atcgcaacgt gatgggcgtt
     2461 ccggcagtgt tcgtaaacgg gaaagagttt ggtcagggcc gcatgacgtt gactgaaatc
     2521 gttgccaaaa ttgatacggg cgcggaaaaa cgtgcggcag aagagctgaa caagcgtgat
     2581 gcttatgacg tattaatcgt tggttccggc ccggcgggtg cagctgcagc aatttactcc
     2641 gcacgtaaag gcatccgtac cggtctgatg ggcgaacgtt ttggtggtca gatcctcgat
     2701 accgttgata tcgaaaacta catttctgta ccgaagactg aagggcagaa gctggcaggc
     2761 gcactgaaag ttcacgttga tgaatacgac gttgatgtga tcgacagcca gagcgccagc
     2821 aaactgatcc cagcagcagt tgaaggtggt ctgcatcaga ttgaaacagc ttctggcgcg
     2881 gtactgaaag cacgcagcat tatcgtggcg accggtgcaa aatggcgcaa catgaacgtt
     2941 cctggcgaag atcagtatcg caccaaaggc gtgacctact gcccgcactg cgacggcccg
     3001 ctgtttaaag gcaaacgcgt agcggttatc ggcggcggta actccggcgt ggaagcggca
     3061 attgacctgg cgggtatcgt tgagcacgta acgctgctcg aatttgcgcc agaaatgaaa
     3121 gccgaccagg ttctgcagga caaactgcgc agcctgaaaa acgtcgacat tattctgaat
     3181 gcgcaaacca cggaagtgaa aggcgacggc agcaaagtcg ttggtctgga atatcgagat
     3241 cgtgtcagcg gcgatattca caacatcgaa ctggccggta ttttcgtcca gattggtctg
     3301 ctgccgaaca ccaactggct ggaaggcgca gtcgaacgta accgcatggg tgagattatc
     3361 attgatgcga aatgcgaaac caacgtcaaa ggcgtgttcg cagcgggtga ctgtacgacg
     3421 gttccgtaca agcagattat catcgccaca ggcgaaggtg ccaaagcctc tctgagtgct
     3481 tttgactacc tgattcgcac caaaactgca taagaagaag taagattcac ctgcaattgc
     3541 ttagccgccg gggtcaaacc tggcggcttt tttatggcat taaaaagccc tgccgggatg
     3601 gctccgggca gggcgggata tttattctgg caattaacgc acaaccagca ccggcagatt
     3661 ggcatggcgg atcacgctcg aggcgttaga acctaacaga tgggtcgaaa tcgatgggtt
     3721 gcgagaacca ataactacaa catcagcccc cagttcttct gccaactcat tgacttcatc
     3781 ccgcacgcta ccaaaacgga catgttgttt aatgcgggaa ggatcgatgg tgaagtggct
     3841 gaccatcgtt tgcagacgtt cttctgcttc atgttgcaga tgctcttcaa aacgacgcac
     3901 atcagcggca aaacggtgca ggctcaggct ggctgacccg ggtagtacgt gaagtagatg
     3961 aataactccg tcatcctggg cgaggaattc agcgtggcga acagctttgt cgctcaattc
     4021 catttcaaat acatcaactg gcataatgat tgtcttatac ataacccttt ctccctgtta
     4081 atcatgaaca aatcattcgc catgattata atatttatcc ctgatatttg tctggttctt
     4141 tttccttacg aacggtttct gtgattaata tattctcact gaacaccagg aattctccca
     4201 aaacctgcgg taccgcccgt tttcccgctg tgatagctac ccttaaagac tgactctttt
     4261 ttgaactgtc tctggaggtt gcatgaaagc attgacttat cacggcccac atcacgttca
     4321 ggtagaaaat gttcccgatc cgggcattga acaggcagat gatattattc tgcgtattac
     4381 ggcaacggcg atctgtggct ctgacctcca tctttatcga ggcaaaatac cccaggttaa
     4441 acatggcgat atttttggtc atgaatttat gggggaagtc gttgaaaccg gaaaggacgt
     4501 aaaaaatttg caaaaaggcg accgggtggt aattccgttt gtcattgctt gtggcgactg
     4561 ttttttctgt cgaatgcagc aatatgccgc ctgcgaaaat accaatgcgg gtaaaggcgc
     4621 tgcgctcaat aaaaaacaga taccagctcc cgcggcattg tttagttata gtcacctgta
     4681 tggtggcgtt cctggtggac aggcggaata tgtccgcgtc cctaaaggga atgtggggcc
     4741 gtttaaagta ccgcctttgc tttcagatga taaagccctt ttcctttctg atattctgcc
     4801 aacggcatgg caggcagcaa aaaatgcgca gatccaacaa ggttcaagcg ttgcagtcta
     4861 tggtgccggt ccagtgggat tgttgacaat cgcctgtgca cggttgctcg gtgcggaaca
     4921 gatttttgtt gttgatcatc atccctaccg cttgcgtttc gccgctgacc gctacggcgc
     4981 gatcccgatt aatttcgatg aagatagcga tccggcacag tcaattattg aacaaacggc
     5041 aggtcaccgg ggcgtggatg cagtaataga cgccgtcggt tttgaagcga aaggcagcac
     5101 cacggaaacg gtgctgacta acctgaaact ggagggcagc agcggtaaag cgttgcgcca
     5161 gtgtattgcg gcggtcaggc gtggcggcat tgttagcgta ccgggcgtct acgc
//
LOCUS       DAZGOB010000215         5123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGOB010000215 DAZGOB010000000
VERSION     DAZGOB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.215"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..834
                     /locus_tag="ACHLIF_004841"
     CDS             <1..834
                     /locus_tag="ACHLIF_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9608683.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVANNITQSG
                     GTEWSKRDKSTYDLTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGS
                     HSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDAFVSVQL
                     A"
     gene            876..1271
                     /locus_tag="ACHLIF_004842"
     CDS             876..1271
                     /locus_tag="ACHLIF_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9608684.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            1283..1657
                     /locus_tag="ACHLIF_004843"
     CDS             1283..1657
                     /locus_tag="ACHLIF_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9608685.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRRWKGDVWP"
     gene            1648..2226
                     /locus_tag="ACHLIF_004844"
     CDS             1648..2226
                     /locus_tag="ACHLIF_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9608686.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            2223..2618
                     /locus_tag="ACHLIF_004845"
     CDS             2223..2618
                     /locus_tag="ACHLIF_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9608687.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSELITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            2626..3366
                     /locus_tag="ACHLIF_004846"
     CDS             2626..3366
                     /locus_tag="ACHLIF_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9608688.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATDMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            3382..3804
                     /locus_tag="ACHLIF_004847"
     CDS             3382..3804
                     /locus_tag="ACHLIF_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9608689.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            3786..4220
                     /locus_tag="ACHLIF_004848"
     CDS             3786..4220
                     /locus_tag="ACHLIF_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9608690.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            4213..>5123
                     /locus_tag="ACHLIF_004849"
     CDS             4213..>5123
                     /locus_tag="ACHLIF_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO9608691.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARA"
BASE COUNT         1134 a         1311 c         1641 g         1037 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaaacccaa gcatgaagtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cacagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcctgcag aacatgcgag acgaagagct ggccattgct
      181 caggtcgaag agatgcaggc agtttctgcc gtgcttaagg gcaaatacac catgaccggt
      241 gaagccttcg atccggttga agtggatatg ggccgcagtg tggcgaacaa catcacgcag
      301 tccggcggta cggagtggag caagcgtgac aagtccacgt atgacctgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggtgtg gtgaatatca tcgtgtttga tccgaaaggc
      421 tgggcgctgt tccgttcctt caaagccgtc aaggagaagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcgaag cggtgtccta taaggggatg
      541 tatggcgata cggcgatcgt cgtgtattcc ggacagtacg tggaaaacga cgtcaaaaag
      601 aacttccttc cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgcacc
      661 tatggctgca ttcaggatgc ggacgcacag cgcgaaggta ttaacgcctc tgcccgctac
      721 ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca
      781 ccgctgatgc tgctggctga ccctgatgcg ttcgtgtccg tacaactggc gtaatcatgg
      841 cccttcgggg ccattttctc tctgtggagg agtccatgac gaaagatgaa ctgattgccc
      901 gtcttcaggt gctgggtgag caactgaacc gtgatgtcag cctgacgggg acgaaagaag
      961 aactggcact ccgtgtggca gagctggaag aggagcttga tgacacggat gacgctgccg
     1021 gtcaggacac atctgtcagc ccggaaaatg cgctgaccgg acatgaaaat gaggttgtat
     1081 cagcgcagcc ggataccgtg actgatacgg ctgatctggt cacggttgtg gcactggtga
     1141 cgctgcatac tgatgcactt cacgccacgc gggatgaggc tgtggcattt gtgctgccgg
     1201 gaacggcgtt ccgtgtctct gccggtgtgg cagctgaaat gacagagcgc ggcctggcca
     1261 gaatgcaata acgggaggcg ctgtggctga tttcgataac ctgttcgatg ctgccattgc
     1321 ctgcgccgat gaaacgatac gcgggtacat gggaacgtca gccaccatga catccggtga
     1381 gcagtccggt gctgtgatac gtggtgtttt tgatgaccct gaaaatatca gctatgccgg
     1441 acagggcgtg cgcgttgaag gctccagccc gtccctgttt gtccggactg atgatgtgcg
     1501 gcagctgcgg cgcggcgaca cgctgaccat cggtgaggaa aacttctgga tagaccggat
     1561 ttcgccggat gatggcggaa gctgtcatct ctggcttggg cggggcgtac cgcctgccgt
     1621 taaccgtcgc cgctggaagg gggatgtatg gccataaaag gtcttgagca ggccgttgaa
     1681 aacctcagcc gtatcagcag aacggcggtg cccggtgccg ccgcaatggc cattaaccgc
     1741 gttgcttcat ccgcgatatc gcagtcggtg tcacaggttg cccgtgagac aaaggtacgc
     1801 cggaaactgg taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc
     1861 agaatcaagg ttaaccgggg ggatttgccc gtaatcaagc tgggtaatgc gcgggttgtc
     1921 ctttcgcgcc gcaggcgtcg taaaaagggg cagcgttcat ccctgaaagg tggcggcagc
     1981 gtgcttgtgg tgggtaaccg tcgtattccc ggcgcgttta ttcagcaact gaaaaatggc
     2041 cggtggcatg ttatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa
     2101 atcccgatgg cggtgccgct gaccacggcg tttaaacaaa atattgagcg gatacggcgt
     2161 gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc aactgaggat ggtaataaag
     2221 cgatgaaaca tactgaactc cgtgcagccg tactggatgc actggagaag catgacaccg
     2281 gggcgacgtt ttttgatggt cgccccgctg tttttgatga ggcggatttt ccggcagttg
     2341 ccgtttatct caccggcgct gaatacacgg gcgaagagct ggacagcgat acctggcagg
     2401 cggagctgca tatcgaagtt ttcctgcctg ctcaggtgcc ggattcagag ctggatgcgt
     2461 ggatggagtc ccggatttat ccggtgatga gcgatatccc ggcactgtca gagttgatca
     2521 ccagtatggt ggccagtggc tatgactacc gacgcgacga tgatgcgggc ctgtggagtt
     2581 cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc tgtaccaaat
     2641 cctgtaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg gagcggtgac
     2701 ccttatgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt taaagacctg
     2761 acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga tgaagatgcg
     2821 gactggactg cgaccgggca ggggcagaaa tcagccggag ataccagctt cacgctggcg
     2881 tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga aggtgatacc
     2941 cgtgcctata aaatccgctt cccgaacggc acggtcgatg tgttccgtgg ctgggtcagc
     3001 agtatcggta aagcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa ggtcaccaat
     3061 gtgggacgcc cgtcgatggc agaagatcgc agcacggtca cagcggcaac cgacatgacc
     3121 gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca ccacgctgac cgtggcattc
     3181 cagccggaag gcgcaaccga caagagcttc cgtgcggtgt ctgcggataa aacaaaagcc
     3241 accgtgtcgg tcagtggtat gaccatcacc gtgaaaggtg ttgctgcagg caaggtcaac
     3301 attccggtcg tatccggtaa tggtgaactt gctgcggttg cagaaatcac tgtcaccgac
     3361 agttaatccg gagagtcagc gatgttcctg aaaaccgaat catttgaaca taacggcgtg
     3421 accgtcacgc tttctgaact ttcagccctg cagcgtattg agcatctcgc cctgatgaaa
     3481 cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttacagtgga agacgccatc
     3541 agaaccggtg cttttgtggt ggcgatgtcc ctgtggcata accatccgca gaagacaaag
     3601 cagccttcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac cacctggccc
     3661 acagaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtccggtat gtatgagttt
     3721 gtggtgaatg atgcccctga acaggcagag gacgccgggc ctgcagagcc tgtttctgcg
     3781 ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact ggcgcgcgag atggggcgac
     3841 ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac tggcaccgct
     3901 tttacagtac ccattatttt catgatgttc tgctggatat gcacttttcc gggctgacgt
     3961 acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat ttcagtctgc
     4021 tgaaccggcg tgaggctgac gaagagcctg aagatgatgt gttgatgcag aaagcggcag
     4081 ggcttgccgg aggcgtccgc tttggcccgg acgggaatga agttatcccc gcttccccgg
     4141 atgtggcgga catgacggag gatgacgtaa tgctgatgac agtatcagaa gggatcgcag
     4201 gaggagtccg gtatggctga accggtaggc gatctggtcg ttgatttaag tctggatgcg
     4261 gccagatttg acgagcagat ggccagagtc aggcgtcatt tttccggtac ggaaagtgat
     4321 gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc gacaggcgct ggctgcacag
     4381 aaagcgggga tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc tgcacagttc
     4441 accgacgtgg ccacgcagct tgcaggcggg caaagtccgt ggctgatcct gctgcaacag
     4501 ggggggcagg tgaaggactc cttcggcggg atgatcccca tgttcagggg gcttgccggt
     4561 gcgatcaccc tgccgatggt gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg
     4621 tatgcctggt atcagggcaa ctcaaccctg tccgatttca acaaaacgct ggtcctttcc
     4681 ggcaatcagg cgggactgac ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg
     4741 gcagggctga cgtttaacca gaccagcgag tcactcagcg cactggttaa ggcgggggta
     4801 agcggtgagg ctcagattgc gtccatcagc cagagtgtgg cgcgtttctc ctctgcatcc
     4861 ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg
     4921 gggctgacgg cgatggcacg ccagttccat aacgtgacgg cggagcagat tgcgtatgtt
     4981 gctcagttgc agcgttccgg cgatgaagcc ggggcattgc aggcggcgaa cgaggccgca
     5041 acgaaagggt ttgatgacca gacccgccgc ctgaaagaga acatgggcac gctggagacc
     5101 tgggcagaca ggactgcgcg ggc
//
LOCUS       DAZGOB010000216         5103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGOB010000216 DAZGOB010000000
VERSION     DAZGOB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.216"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..281)
                     /locus_tag="ACHLIF_004850"
     CDS             complement(<1..281)
                     /locus_tag="ACHLIF_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO9608692.1"
                     /translation="MFGIFGKKARRAAVEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQILRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALREI"
     gene            complement(471..680)
                     /locus_tag="ACHLIF_004851"
     CDS             complement(471..680)
                     /locus_tag="ACHLIF_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608693.1"
                     /translation="MFVSEVLMEDKDNKGWVKGWAVVRSSPWHLVGVFATEEDAETEA
                     RKMGDKYEVHYGSHRTGSDDFVWGE"
     gene            complement(733..957)
                     /locus_tag="ACHLIF_004852"
     CDS             complement(733..957)
                     /locus_tag="ACHLIF_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608694.1"
                     /translation="MKKLMVTIGPFETEVSFRVVQGESVLVEDVFHGKSTGPYVKEYL
                     IDATDENIEVVYDSVNHPDLIIKAKLKPLY"
     gene            complement(1048..1124)
                     /locus_tag="ACHLIF_004853"
     tRNA            complement(1048..1124)
                     /locus_tag="ACHLIF_004853"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1088..1090),aa:Arg,seq:tct)
     gene            complement(1138..1214)
                     /locus_tag="ACHLIF_004854"
     tRNA            complement(1138..1214)
                     /locus_tag="ACHLIF_004854"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1178..1180),aa:Arg,seq:tcg)
     gene            complement(1222..1297)
                     /locus_tag="ACHLIF_004855"
     tRNA            complement(1222..1297)
                     /locus_tag="ACHLIF_004855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1262..1264),aa:Ile,seq:cat)
     gene            complement(1802..2554)
                     /locus_tag="ACHLIF_004856"
     CDS             complement(1802..2554)
                     /locus_tag="ACHLIF_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9608695.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSSLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEIQLRKVTR"
     gene            complement(2568..3617)
                     /locus_tag="ACHLIF_004857"
     CDS             complement(2568..3617)
                     /locus_tag="ACHLIF_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9608696.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSVFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIALPSHTHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            complement(3619..3888)
                     /locus_tag="ACHLIF_004858"
     CDS             complement(3619..3888)
                     /locus_tag="ACHLIF_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608697.1"
                     /translation="MAHDSRLHNSAALASRQGRCSHVFKTDWFQHPLCTEEQAEWLIQ
                     CYRRRGYEFQKDLSFDRRHWIISARLPYSERPPRPSRTFQQRIWR"
     gene            complement(3942..4136)
                     /locus_tag="ACHLIF_004859"
     CDS             complement(3942..4136)
                     /locus_tag="ACHLIF_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608698.1"
                     /translation="MKIPASLIPGFYESTRPVVVYRNSDGSFQCGFVMRSNEVVVSLS
                     LLSEVRELAGLPVDDIQKQL"
     gene            4393..4764
                     /locus_tag="ACHLIF_004860"
     CDS             4393..4764
                     /locus_tag="ACHLIF_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9608699.1"
                     /translation="MRIFKNAWFERFARKHRISDKSLRKIVEQADKGIISANLGSGVI
                     KQRLARSGGGKSGGYRTIIFYRVAEKAFFIYAYAKNERENITAIEENAFRKAAPHVLN
                     LTDEQLAQLIQQGQITEVPNE"
     gene            4757..5074
                     /locus_tag="ACHLIF_004861"
     CDS             4757..5074
                     /locus_tag="ACHLIF_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9608700.1"
                     /translation="MSKNYRSDALASVHEMMESLHDIGAITKQTMREFDETCLQPAPV
                     MSPERIRALREREHLSQPVFARYLNVSKNLISDWERGVKRPGGAALRLLSVVEKNGIQ
                     VIS"
BASE COUNT         1344 a         1330 c         1117 g         1312 t
ORIGIN      
        1 tcaatttcac gcaatgccgc tcgacgacca attttaaagt tcgtatccag ctgaccgata
       61 atggttgcgc taatcgcatt aatttctgac gtaaacgcgg acaacgcagg ctggttacgc
      121 aagatctgct cgatcttcgc tttctctgaa gcctcacatt caccatctgc ataggccacc
      181 agataggcag cattaataac cgcctgtgcc agatcacgtt tctcaaactt tttaatttcc
      241 actgccgctc ggcgggcttt tttaccaaaa ataccaaaca tcgtgacgtt cctttgggtg
      301 ggtgagccaa cgcccgggag cgatctgccc acagagaaag tcacactgac cactccgtaa
      361 gctcaccccc gaaaggctct gtggttgata tgcgccgggc gtggcgcgga tacaaaaaag
      421 gccgccaata gcgacctcag ttacgggatt attctggggt taaacgactg ttactccccc
      481 cagacaaaat catcacttcc tgttcgatgc gagccatagt gaacctcgta cttatctccc
      541 atctttcttg cttccgtttc tgcgtcttcc tctgtcgcaa aaaccccaac aagatgccag
      601 ggcgagcttc ttaccacagc ccaaccttta acccatcctt tgttgtcctt atcttccatt
      661 aacacttcag aaacaaacat atttatctcc ttgtgggtac ccagagatat tttatgattg
      721 ctcccggtca gatcaataaa gtggcttcaa ttttgcctta atgatcaaat cagggtgatt
      781 gacggaatcg tacaccacct caatattttc atccgtggcg tcgataagat attcttttac
      841 ataaggacct gttgattttc catgaaatac atcttcaaca agtacactct ccccctgaac
      901 aacacgaaaa ctaacttctg tttcgaacgg accaatcgtc accatcagtt ttttcacata
      961 gcctcctgat aagcactcga tttattagtt aatggtgtaa cgcagataca aaaaaaggcc
     1021 cgcaaaagcg agccaagtaa ataaatatgg cgcgttgtac tggattcgaa ccagtgaccg
     1081 attgcttaga aggcaattgc tctgtccgac tgagctaaca acgcatgatg ctgataatgg
     1141 accgccatcg ggaacttgaa ccccgcacag ccagcttcga aggctgacgc tctatcccga
     1201 tgagctaatg gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat
     1261 tatgagtcgc tcgctctcac cactgagcta aagggccggg cgcaggataa taacggtacg
     1321 taactaattc tgcaatatca tccgttctga ctgactacat tctgaacttc cctgaccgtc
     1381 tgctcaaaac gcccgctctc cagctcaacg ccaattgcac gacgccccag cgccattgct
     1441 gctttgacgc tacggacata aaaaagccag ccactggggg aggctggcga aactcgtaga
     1501 gcaaaagtgt tgttacacta acttcgtcac agggttatcc tgcaatactc aaaatacaca
     1561 atatttacaa aactaatagt atacaaggcg atctttaaga ttttgttatt aattgtggtc
     1621 gcaccttcct ttctgtgtac tttccgtata gctcacagga ttctgggtac aaaaaaaccc
     1681 gcgcatcggc gggttcttaa atcttatcaa cggtagacat acaaagccca tcattgggaa
     1741 aatcttatcc atattttttg aaaaatgcaa gcatcatgtc gtcatcttcg gcgaaaacca
     1801 tttatcttgt cacctttctc aattgtatct ctgcatatgc ttcttcctgc cagcactttg
     1861 taaccagttt atcaatgaca tctgcatatc ctttgtacca ctgataatcc gtcaggtctg
     1921 gtaccagctt ctggacatga agccgcgcca gtgtggttgg taaactgcta aaccggtttc
     1981 cattgcaacg cccacaaacc ttataaacag gcgtgccatg aagccgggtt cttttttcat
     2041 ccaggacaat acctttaccc ttgcaccctc tgcatgctgt gctgacttct cccttaccat
     2101 gacaatgctg acatagttcc ttcacccact cttccttaat aacagattcc ccgcttctgg
     2161 agtgtttcac cacctcgcgc aatacattat gaaatccagt accagcacaa tgctcacagc
     2221 gagccttact tgccgcagac ctggaataat cagcaaaggc aaaattcaca aggtaaggaa
     2281 tgatctgtag ccgggtttct tcactcaatt tattcaatgt cgggttatcc agtgccatcg
     2341 cgtaattgag cagaccttca atcgcaaact gaggatcctg aacaccaact tttgccagga
     2401 ataaagcaaa cccaagcggt gcttttgact gcaccatccc ctgcgcagcc atcacatccg
     2461 taattgttaa accaccagag cctgtcgccg gtgcgtcatc gctcagtttt ggagatttcg
     2521 gggagtaata tttcggtaag gcttcaaggt tcatgctcgt tctccactta cgccagtacg
     2581 ccaattgcca gcgcacgatc gataaaacga aatatcagct ccagctgaga gccatacttc
     2641 tcttcaaatg ccacggtatc cgcatgaagc tcgttgtgat gctcccgaca cagcggtagc
     2701 gtgaaaatat cgtgagattt tgtccccatt ccgccctgac catgaccaat caggtgatgg
     2761 ggatcgtcgg ctggcttacc acaacacgca cacggctgtg tctttaccca gcgtgtgtat
     2821 ttctcattta cccagcggcg acgtttaggt cgtttcatga aggattccgg agactccgga
     2881 tcaacggcaa tgctgaccac cgtcttttcc tgtggtgggt tctgttgctg gtgggtgtga
     2941 gacggtagcg caatattttt tgtgcgctgc ttcagtatgc tggtggcggt ctgttctccc
     3001 ggtatgatgt cgctttcacg gtatacggag cggatttttt ccgcacgcaa ccccagcgaa
     3061 cgacgtaata ccgcttccgg tagcgcgtcc gccacctgat tgcggaccgc ccaccaggat
     3121 aattcagcca gcgataattc ccgttcctgt atgccattca ttgcgtggcg gatgacgtca
     3181 atcatccatg ctgacaggtt ttgttgagca agttgctcga gtgattctga tgtctggtcg
     3241 cgcagctggt tgtcacagtg ccagcacaac accatcgcgc cggtaccata acggtgaatg
     3301 actgtttcgc tgtgatgata atcgccgtgt ggccactggc aggatttcac gtgacgtaat
     3361 aaccagtcag acagtgcacc tgcaccacct gctgcacgaa taacccgctc atcgctgaaa
     3421 aatggcagta atgttttatc ctctgccagc ggctggcgaa cggcaggaac gactccggac
     3481 ggcagaccgc gcatgttttt cggttccggc tccaccaata ttctgccgtt atggaatact
     3541 gacattgatt cacggcctgg cttaacgata accagaccga gttccggtac cagaacaggt
     3601 cgaagtaata cccgcacgtt acctccagat gcgctgctgg aatgtgcggg acggacgcgg
     3661 tgggcgttcg gaataaggga gcctggcgga gattatccag tgacgacgat cgaagctgag
     3721 atctttctga aactcgtaac cacgcctgcg gtaacactgg atcagccatt cggcctgttc
     3781 ttcagtgcat aggggatgct ggaaccagtc ggttttaaat acgtgcgaac accgtccttg
     3841 cctactggca agggcggcag aattgtgcaa tctgctatcg tgcgccatcg ggatctccgg
     3901 tggcacggtg tttctcagcg acggttcaag tcagcctgat tttatagctg cttctgaata
     3961 tcatcaacag gtaatcctgc caactctctt acctcagaaa gaagagaaag gcttacaaca
     4021 acctcattac ttcgcataac aaaaccacat tgaaaagaac catcactgtt tctgtaaaca
     4081 acaaccgggc gcgtgctctc ataaaacccc gggatcaaac tggctgggat tttcacaaca
     4141 cctcctgacg taaaggaaat gaaatgcatt atcgcttctc tcggactata accatgaaaa
     4201 gagacgcatt tcactcagta aatctgagga ttttatgcgc aagaacaatg actttttctg
     4261 tctgccgttt atacaatctg aattacgtct gttttttgaa caccgataaa tcagcaagca
     4321 ttctcaaata aagattgcct ccatagtcca catggtgtaa cactatgtgt tatataaaat
     4381 gcagaggcag gtatgcgaat tttcaaaaac gcctggtttg aacgtttcgc tcgaaaacat
     4441 cggatttccg ataaatcgct gcgcaaaatc gtggagcagg ccgataaggg gatcatatcc
     4501 gcaaatttgg gtagtggtgt cattaaacaa agattagccc gaagtggtgg cggaaaatca
     4561 ggcggttacc ggacaataat tttttaccgc gttgcagaaa aagccttttt catctacgca
     4621 tacgcaaaga atgaacgaga gaatatcact gctatagagg aaaatgcttt tcgaaaagcc
     4681 gctccccatg tcctcaatct tactgatgaa cagctggcac aattgattca acaaggccag
     4741 atcacggagg tacccaatga gtaaaaatta ccgcagtgat gcacttgcat ctgtacatga
     4801 aatgatggag tcactccatg atatcggtgc aatcacaaaa caaactatgc gcgaattcga
     4861 tgaaacttgt cttcagcctg cgccggtaat gtctccggaa aggatccgtg cactgcgaga
     4921 acgagagcat ctgtctcaac ctgtttttgc cagatacctc aacgtcagta aaaacctgat
     4981 atcagactgg gaacgaggag tgaaacgccc gggaggtgca gctcttcggc ttctttcagt
     5041 tgtcgagaaa aacgggatcc aggtaatatc ctgatattct aatacaacaa aacccgccga
     5101 agc
//
LOCUS       DAZGOB010000217         5066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGOB010000217 DAZGOB010000000
VERSION     DAZGOB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.217"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            17..538
                     /locus_tag="ACHLIF_004862"
     CDS             17..538
                     /locus_tag="ACHLIF_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9608701.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            596..829
                     /locus_tag="ACHLIF_004863"
     CDS             596..829
                     /locus_tag="ACHLIF_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO9608702.1"
                     /translation="MSEYFRILQGLPDGPFTREHAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            893..2857
                     /locus_tag="ACHLIF_004864"
     CDS             893..2857
                     /locus_tag="ACHLIF_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO9608703.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGILTADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTTRQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEYLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEIRAGSGVVVSWCYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSSPARIYLECGHYSLTSAAPSGKEGTAFMALMAEHFRL
                     AALLPDEWERDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIDALNDAGLSGAARDAEKMNKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQAETETTEYRDNQADAA"
     gene            2926..3360
                     /gene="psiB"
                     /locus_tag="ACHLIF_004865"
     CDS             2926..3360
                     /gene="psiB"
                     /locus_tag="ACHLIF_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO9608704.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3357..4119
                     /locus_tag="ACHLIF_004866"
                     /pseudo
     CDS             3357..4119
                     /locus_tag="ACHLIF_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            4298..4447
                     /gene="mok"
                     /locus_tag="ACHLIF_004867"
                     /pseudo
     CDS             4298..4447
                     /gene="mok"
                     /locus_tag="ACHLIF_004867"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4389..4514
                     /locus_tag="ACHLIF_004868"
     CDS             4389..4514
                     /locus_tag="ACHLIF_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO9608705.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(4812..5066)
                     /locus_tag="ACHLIF_004869"
                     /pseudo
     CDS             complement(4812..5066)
                     /locus_tag="ACHLIF_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1098 a         1345 c         1590 g         1033 t
ORIGIN      
        1 cagaaggaga cacatcatgg cagttcgtgg aattaacaag gtcattcttg tcggtcgtct
       61 gggaaaagat ccggaagtcc gttacatccc caacgggggc gcagtggcaa acctgcaggt
      121 ggccacgtca gaaagctggc gtgacaaaca gacgggggag atgcgggagc agacagaatg
      181 gcatcgcgtg gtgctgttcg gcaagctcgc ggaagtggca ggtgaatatc tgcgtaaggg
      241 cgcgcaggtc tacatcgagg ggcaactccg tacccgtagc tgggaagata acggtattac
      301 ccgttacgtc actgaaatcc ttgtgaaaac cacgggcacc atgcagatgc tggggagtgc
      361 accacagcag aacgctcagg cacaaccgca gcctcagcag aatggtcagc cacagagtgc
      421 tgacgcgacg aaaaaaggtg gcgcgaaaac gaaaggccgt ggacgtaagg ccgcgcagcc
      481 ggagcctcag cagccgtctg aacccgcgta tgacttcgat gacgatattc cgttctgaac
      541 gggctgactg tgataaccgc cccgtcctgt gcggggcatc accggagaga tggggatgag
      601 cgaatatttc agaatacttc agggactgcc ggacggtcct tttacccgcg aacatgccga
      661 agccgttgcc gcacagtacc ggaacgtctt tatcgaggat gaccacgggg aacattttcg
      721 cctggttgtc cgtaataacg gaacaatggt ctggcgtacc tggaattttg aggacggtgc
      781 cgggtactgg atgaaccatg tcatccggga tttcggcatt cttaagtaag agaggtgccg
      841 gacgggaacg cgtccggcag acatacaacc atcccggtca aaggagaaat atatgtcagt
      901 tacagagtct aagacaaaaa cggagcgtaa atccagtcgt aaacctgcaa aaacgcagga
      961 aacagtcctg tctgccctgc tggcgcagac ggaggaagtg agcgtgccgc tggcctcact
     1021 gattaaatca ccgctgaatg tgcgcacggt gccgtattct gcggagtccg tcagcgaact
     1081 ggctgagtcc attaagggcg ttggcctgtt gcagaatctg gtcgtgcata ccctgcctgg
     1141 tgaccgttac ggtgtcgccg caggtggtcg ccgactggct gcactcaaca tgctggcaga
     1201 gcgtggcatc cttacggctg actggcctgt acgcgtgaag gttattccgc aggaactggc
     1261 gactgccgca tcaatgactg aaaacggtca tcgtcgggat atgcaccctg ccgaacagat
     1321 tgccggattc cgcgcaatgg cgcaggaagg caaaacacct gcgcagactg gcgacctgct
     1381 gggctattca ccccgccacg ttcagcgaat gctgaaactg gctgaccttg cgcctgtcat
     1441 cctcgatgcg ctggcagaag accgcatcac caccgagcac tgtcaggcgc tggcgctgga
     1501 gaacgacacc acgcgtcagg tgcaggtgtt tgaagccgcc tgtcagtcgg gatggggcgg
     1561 taaaccggat gtgcgggtta tccgcaatct gattacggaa agtgaagtgg cggtggcggg
     1621 gaacagtaaa ttccgcttcg tgggggctga tgctttctca cctgacgaac tgcgtactga
     1681 tttgttcagc gatgacgggg acggttatgt ggaccgtgtg gcgctcgatg ctgccctgct
     1741 ggaaaaactc caggctgtcg cagagtacct tcgggaagcc gaaggctggg agtggtgcgc
     1801 cgggcgcatg gagcctgtcg gtgagtgtcg tgaggatgcc ggaacatacc gctgtctgcc
     1861 ggagccggaa gcggtgctga cggaggcgga agaagaacgc ctgaacgaac tgatgacgcg
     1921 ttacgacgcg ctggaaaatc agtgtgagga atccgacctg ctggaagcag aaatgaagct
     1981 gatgcgctgc atggcgaagg tcagggcgtg gacgccggag atacgtgccg gaagcggtgt
     2041 ggtggtgtcc tggtgttatg gcaacgtatg tgtccagcgt ggtgtgcagt tgcgcagtga
     2101 ggatgacgtg gctgacgacg ctgaccgcac ggaacaggtg caggagaaag catcagtgga
     2161 ggaaatcagt ctgccactgc tgacgaaaat gtcttccgaa cgcacgctgg cagtccaggc
     2221 ggcactcatg cagcaaccgg acaaatctct ggcactgctg gcatggacgc tctgcctgaa
     2281 tgtgtttggc agcggggcgt acagtagtcc cgccagaatt tacctggaat gtgggcatta
     2341 ctcactgacc agcgctgcgc cgtcagggaa ggaaggtacc gcattcatgg cgctgatggc
     2401 agagcacttt cgccttgctg ccctgctgcc ggatgaatgg gaacgggaca tgacgacgtt
     2461 cctgtcactc agccaggagg ttctgttatc cctgctcagt ttctgcactg catgcagcat
     2521 ccacggcgtt cagacccgtg agtgtggtca cacgtcacgc agtccgctgg actcgctgga
     2581 aacggctatc ggattccaca tgcgcgactg gtggcagccg acaaaagcaa acttcttcgg
     2641 acacctgaaa aagccgcaga ttatcgacgc cctgaatgac gccggactgt ccggtgctgc
     2701 acgggacgcg gagaagatga ataaagggga cgcggctgaa catgcagagc accacatgaa
     2761 agacaaccgc tgggtgcctg gctggatgtg tgcgccacgt ccacaggcag agacggaaac
     2821 cacggaatac cgtgacaacc aggccgatgc cgcctgaccg ccagttataa accatttatc
     2881 acgccgcccc gccggagacg gggcggcagc aagggagata ccgtgatgaa aactgaactg
     2941 accctgaatg tattacagac catgagcgca caggaatatg aagatatccg ggctgccgga
     3001 agtgatgaac gtcgtgagct gacacacgcc gtgatgcggg agctggatgc gccggataac
     3061 tggacgatga acggcgagta cggcagcgag tttggcggat ttttccccgt tcaggtccgt
     3121 ttcacgcctg cccacgaacg ttttcacctg gcgttatgtt caccgggcga tgtctctcag
     3181 gtctgggtgc tggttctggt gaatgccggt ggtgagccgt ttgcggtggt tcaggtacag
     3241 cggcggtttg caccggaagc cgtcagccat tcactggcac tggcggcgtc actggacgcg
     3301 caggggtaca gcgtcagtga catcatccat atcctgatgg cggaaggagg tcaggcatga
     3361 gcgcacgttc acaggcactg gttcctctca gcacagagca acaggccgca tggcgggcgg
     3421 tggcagagac cgaaaaacgc cgccatcagg gtaacacgct ggctgaatat ccgtatgcgg
     3481 gggcgttttt ccgctgtctg aacggaagcc gcaggatatc gctgtctgac ctgcgatttt
     3541 tcatgccgtc cctgaccgca gaagaactgc acggcaaccg tcttcagtgg ctgtatgcca
     3601 tcgatgtgct gattgaaaca cagggcgagg tttgcctcct tccccttcca ggcgatgcag
     3661 cagaacgctt gttcccgtcg gtgcggttcc gtgtcaggga acgccgtcgc cataaatccg
     3721 cgctggtcat gcagaagtac agccgccagc aggcacggga ggcagagcag aaagcccgtg
     3781 cgtaccaggc gctggtcgct caggctgaaa tcgaactggc attccattcg ccggaaacag
     3841 tcggaagctg gcatgcacgc tggagtgacc gggtggcaga gcatgacctg gaaaccctgt
     3901 tctggcagtg gggcgagcgt tttccgtcac tggccggaat ggaacgctgg cagtggcagg
     3961 atatgccgtt ctggcaggtt attgcggaag ccagtcttgc agcaagagag gccggtcacg
     4021 ctgtccgtga gatggagcgg tggatggtgc cgaacaaact ccgggaggca gcgtgatgcg
     4081 gcaacaatca cacggatttc ccgtgaacgg actgaatgag cggattattt tcagggaaag
     4141 tgagtgtggt cagcatgcag gtatatgggc tatgatgtgc ccggcgcttg aggctttctg
     4201 cctcatgacg tgaaggtggt ttgttgccgt gttgtgtggc agaaagaaga tagccccgta
     4261 gtaagttaat tttcattaac caccacgagg catccctatg tctagtccac atcaggatag
     4321 cctcttaccg cgctttgcgc aaggagaaga aggccatgaa actaccacga agttcccttg
     4381 tctggtgtgt gttgatcgtg tgtctcacac tgttgatatt cacttatctg acacgaaaat
     4441 cgctgtgcga gattcgttac agagacggac acagggaggt ggcggctttc atggcttacg
     4501 aatccggtaa gtagcaacct ggaggcgggc gcaggcccgc cttttcagga ctgatgctgg
     4561 tctgactact gaagcgcctt tataaagggg ctgctggttc gccggtagcc cctttctcct
     4621 tgctgatgtt gtacgggcat gaacaacctg acttcaggaa ggtcgtttcc cttcaggaac
     4681 ggggatgaac gtgcgcctgc ggcgcccgtt cttttccccc gccttctctc tttatggcct
     4741 gtcggaaatc acatcacctt ccctgtgatt attctctttt ccctcctgtt ctgattctga
     4801 ttactgctgt cctgattact gtctgttttg ttgtcctcct gcctgtgctt ttcccgggcg
     4861 tccccctggc ggggcgggct gtcgtgaacg gagccagcga agctgtctcc gccctggcgg
     4921 gcttccatcc ctgacgcgaa acccgggctt gcagcgaccg ttccggtcgc gccgcctctg
     4981 tcgcgccgtg gcctgcgctc cgggcatccc ggcaaaaggc cgccgggatg taaggcgcaa
     5041 cgccgtttcg caaaccgcgc ttcacg
//
LOCUS       DAZGOB010000218         5063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGOB010000218 DAZGOB010000000
VERSION     DAZGOB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.218"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            133..435
                     /locus_tag="ACHLIF_004870"
     CDS             133..435
                     /locus_tag="ACHLIF_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO9608706.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            438..797
                     /locus_tag="ACHLIF_004871"
     CDS             438..797
                     /locus_tag="ACHLIF_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9608707.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(844..1236)
                     /locus_tag="ACHLIF_004872"
     CDS             complement(844..1236)
                     /locus_tag="ACHLIF_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9608708.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            1363..1623
                     /locus_tag="ACHLIF_004873"
     CDS             1363..1623
                     /locus_tag="ACHLIF_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9608709.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            1620..2057
                     /locus_tag="ACHLIF_004874"
     CDS             1620..2057
                     /locus_tag="ACHLIF_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9608710.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            2144..3154
                     /locus_tag="ACHLIF_004875"
     CDS             2144..3154
                     /locus_tag="ACHLIF_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO9608711.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNSDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            3147..3608
                     /locus_tag="ACHLIF_004876"
     CDS             3147..3608
                     /locus_tag="ACHLIF_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9608712.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKLLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            3642..4367
                     /locus_tag="ACHLIF_004877"
     CDS             3642..4367
                     /locus_tag="ACHLIF_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9608713.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMVISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDIVRQITDSSRRVVSEK"
     gene            4383..4775
                     /locus_tag="ACHLIF_004878"
     CDS             4383..4775
                     /locus_tag="ACHLIF_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9608714.1"
                     /translation="MAKEFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            4772..>5063
                     /locus_tag="ACHLIF_004879"
     CDS             4772..>5063
                     /locus_tag="ACHLIF_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO9608715.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLTPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVA"
BASE COUNT         1385 a         1079 c         1409 g         1190 t
ORIGIN      
        1 ctgcgttctg tggggcgggg tgggtttatg gatacaattt acaaattaaa atttaactgg
       61 tcaacatgtt tattattaaa tttgtaattg cgggctgttg catcagtccc aaaatgggac
      121 tactatacag ttatgaagat tatctcagtt aaaacactca gggatttttg ggcggagaat
      181 cctgatgcag aacaaccgct aagggcatgg gtggatgagg cggcaaaagc tgactggaaa
      241 agcccagcag acattaaggc acaataccga acggccagta tattgaaaaa ccggcgcgtg
      301 gtattcaaca taaaaggtaa tcattaccgt ttaattgttg caattgcgta tcagcgagga
      361 tgggtatttg ttaaattcat cggaaaccac aaggaatacg atgctattga tgctgatacc
      421 atcgaactgg agtaagcatg aacatcaaac ctattcgtac agagcaagat tatgaagccg
      481 cgttgcgtgc tgttgaaccc atgttcgaca atgagcccga aattgacacg cctgaggggg
      541 atttttttga ggtgatgtgt ttgctaatag aggaatatga aaaaaaacat tatcccattg
      601 agccaccatc cccaattgaa gctataagat ttcgcatgga acagcagggg ctgactgtga
      661 aagatttgga acccgcaatt gggaaaaaaa atcgggttta tgaggtgttg aatggtacca
      721 gaagccttac gttaccaatg attcgccgtc ttcataatca atttggtatc cccctggaaa
      781 gcctggttgg attataaaat ctgctagtca tttgcctgat gctcgttcca gaaaaggaat
      841 gcatcaggca gttttgtttt tctgccgcag taactcttca agtttccgtt tatagaaatc
      901 gcgtttttgc tccatatcac gaatgatctg ctctgcgtcg ctttgaggta actcatctaa
      961 aagcgatatg atttttcgtt gttgttctgt aagttgcggt tggttgtcat tactggatac
     1021 agccatttta tcgccgagag tttcttcttc cataaagaac caatggacgg gatgttgtga
     1081 gagctctgct aatttttcca gtttatccat tcttggcatc acgcctttca accaaccttg
     1141 cacggattgg ggttttacac caagacgtct tcccagctct gactggttta tattcaattc
     1201 ctgcaacacc tgctgaaggc gttttacaaa gatcatcacc acccctcgta aactagttcc
     1261 gcgatcctac agaaaaaatt gataagtggc attacaaata gaagttgaaa tttaaattta
     1321 aatttgtaat tatcggtgtc atcgtaaagt tcggagggaa acatgcaaaa aagtactcaa
     1381 gtgaaaatcc tgtcaataat gagccaatca gaattagggc gtcgtcttgg taaaacaccg
     1441 caaaccataa gtgggtggtt taaaaaacga gtgccagcgg aggaggttat accagcatgt
     1501 gaggcgcttg actggggagt aactccgcat gaattgcgcc ctgataaata ccctaatcca
     1561 accgatggtt tacctgttga gtatcaggct aacgcacaag cagcggcggg agttgattca
     1621 tgaaaatcaa gcatgagcac atccgcatgg cgatgaatgc gtggctgctt tatccgaggg
     1681 tagggcgcaa aaaaatcgct gatgatatag cgacagcata ttttgagctt gaaatgactt
     1741 atccaccaat gcatgacacc tctacgacag agggtattgg attgaacata caaaatattt
     1801 ttcgctggct tgaaaaggat acgcctgatg ctgttgaaaa aattcaggca ctaattccag
     1861 ctatcctgac tgttcttccg cgtgaactgc gttatcacct cagtattttt gacactgttg
     1921 agcgccgtgc attactggcg gcccaggaag cgttgagcac ggcaattgat gcgcatgatg
     1981 atgcagtcca ggcagtttac cggaaagcgt atttcagcga cggcgggtca tccggcgagt
     2041 ctgttgtggt gcattgatat ttatgccaga cccctgctga ttctgttgat tggggaatca
     2101 cagaatatca ccagaggatg gttcgtcaca agatgaggca attatggccg cattaccata
     2161 catgcaactg tacatagctg attacctggc tgacaccatg catttgtccg cagaggagca
     2221 cggtgcgtat ttgttgctga tgttcaatta ctggcaaaca gggaaaccaa tacctaaaaa
     2281 caggctggca aaaatttccc gtctgactaa cgagcgatgg gctgatgtgg aaccatcctt
     2341 gcgggagttt ttttgcgata acagcgatga atgggtgcat cttcggattg aggaagatct
     2401 ggcatcagtc agggaaaaat tgaccaaaaa atcagccgcc ggaaaagcat ctgttcaggc
     2461 cagaagaagc agaaaggaag catatgttca aacaaaacaa gagagagatt taacaggtgt
     2521 tcaaacaaat gttggtgttg tgtttgaaca tgacgcaaac acaaaagcaa ctaataaaga
     2581 tacagatcta aaagaattaa accccacaca taacgtgcgt gaacgcgaga gtattccgac
     2641 cagtgagtcg catggtgcgc cgttgcagac agccgaacct gaatacctgg acggcctgag
     2701 cgaaccgatc gggaaatttt cgatgactac tgtctggcag ccgtcgccgg attttcgaca
     2761 acgggcagca gtgtggggta tggctctgcc tgagccggaa tttacacctg ctgagcttgc
     2821 cgcattccgg gattactgga tggcggaggg gaaggttttc acgcaggttc agtgggagca
     2881 gaaatttgcc cgccacgtgc agcacgtcag ggcacaggta aaaccagtca gcaagggggt
     2941 aagccatgca gcatcaggtg gcacggcatc acgggcagtt caggaaatcc gggcagcacg
     3001 cgaacagtgg gaacgtgaaa acggatttat cagcaacgga aacggcctgg aagctgtggg
     3061 agcttatggg ggaggtgtat tcgaaccgct ggactcagaa gaacggggcc gcaccttcga
     3121 agctctggat tgcccagatt ggtgcgatga ctgaacagca aatccgtctg gtctgccgtc
     3181 agtgcatgga ccgctgccgg gcgggtgaaa cgtggccccc ggacctggct gagtttgttg
     3241 cgctgatttc ggagagtggg gcaaatccat ttggtcttac ggtggatgcc gtgatggaag
     3301 agtaccggcg ctggcgcaat gaatcctggc gatacgacgg gagtgataaa tacccgtggc
     3361 cacagcctgt gctgtaccac atctgcctcg aaatgcgtac cagagggatt gagcgccaga
     3421 tgacgcaggg tgagttaaaa ctacttgcag aacggcaact gacgaaatgg gcaaagcatg
     3481 ttggtaacgg gatgagtgtt ccgccagtgc gacgacaact ggaaggggcg aaacacccgc
     3541 aagggccaac gccaattgag cggctgaaac aggaatacga acgccggaag gcagctggtt
     3601 ttatttgaat ctgagaaacg attttgtcgg aggaaatttt aatggaaacc gtatttgacg
     3661 cactgaaagc actgaaaaga gcctcttcac aggtagtggc ggcccgcctt ggaatcagcc
     3721 gtgaagatgc ggtcaacgaa ctgtggaaac tgaagcgccg cggtgaagcg gataacaagg
     3781 ggtcgatgtg gtggctgatt caggctggtg aaagtgaacc ggtgtcaccg gtaccgaaag
     3841 tgacagcgca aatgctgact gaggcgattg aacaacatgg cccacaaacg gcggatgagc
     3901 tggcactgat gttcgggatt acctcccgcc gggcgaattc atcgctggcc atggtaatca
     3961 gcaaagggcg tctgattcgc gtgaatcagg gcggtaaatt tcgttactgc ataccgggcg
     4021 ctgatttacc ggcagagccg gaagctgcat ccgtagcgga aaccgatggt aaagcctttc
     4081 ctcagccagc aggtgttgcg ttaccagttc gggaagcaga aacacaggaa gaaataaaaa
     4141 cggaaagtgt ggcggtcaca gtgcagtcac agccgtcgtt caccagaaaa catccggatg
     4201 gtctgatttt accatcgctg catgtggcta accgcgagct gcgccgggca aaaggtcagg
     4261 ttcagaagtg ggagcgagtc tgcgccgcgc tgcgggagct gaacaagcac cgggatattg
     4321 ttcgacagat tactgattct tcccgccgtg ttgtatcgga aaagtgattg ccggaggcgc
     4381 ttatggcaaa agaatttaca ccagaagagc gggaagaagt gaaggcgcgc attgtggaat
     4441 tcgtgcgcct gagcggacga gaaacttttc gacaactggc agataaaacg ggtgtcagta
     4501 agaccgctat tcgtcgttta tctggtgcgc ttgcggccag tggtgatgtc tggctctctg
     4561 gttgcggggt atttccatca gagcaggcgt atcgcgtatg gcgtaagaca ccggagaagg
     4621 ctgctgaccc gacactgatt cgaaagttac ctgacggaga aatacgtcgt tacaacagac
     4681 ggcagaacat aatttgtcgt gagtgccgcc agagcgaagt tatgcagcgt gtgctggcgt
     4741 tctatcgggg aaactttcag gaggtgatgg agtgagggtg agagtttata ttgccggtcc
     4801 aatgacggga tatgaaaatt tcaaccgtga ggcgtttcac aaggcggaag aggaactgaa
     4861 acgggaaggg cataccgtct taactccggc agtacttccg gacgggctga cacagccgca
     4921 ctacatggat atttgcatgg caatgattcg ttgtgtggat gcgatttaca tgctgaatgg
     4981 ctggcagcgg tcagcgggcg ctaaggcaga gctggcactg gcggagaaac tggggcatgc
     5041 ggtgatttat caggaggtgg ctc
//
LOCUS       DAZGOB010000219         4990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGOB010000219 DAZGOB010000000
VERSION     DAZGOB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.220"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..168
                     /locus_tag="ACHLIF_004880"
     CDS             <1..168
                     /locus_tag="ACHLIF_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO9608716.1"
                     /translation="HGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            611..4513
                     /gene="espP"
                     /locus_tag="ACHLIF_004881"
     CDS             611..4513
                     /gene="espP"
                     /locus_tag="ACHLIF_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFO9608717.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4761..>4990)
                     /locus_tag="ACHLIF_004882"
     CDS             complement(4761..>4990)
                     /locus_tag="ACHLIF_004882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608718.1"
                     /translation="AQNQPAETVRAAAAATGRFAVAFPVRVAPGSGCFMKTNAPRLFF
                     VDKESGVSFDVFHVLSVSFPVTACATHDGGM"
BASE COUNT         1515 a         1133 c         1113 g         1229 t
ORIGIN      
        1 catggtggtg agcggggagc gctactgtac agcctgatcg ggacgtgcaa actgaatgac
       61 gtggatccag aaagctacct tcgccatgtg cctggcgtca tagcagactg gccggtcaac
      121 cgggtcagcg aactgcttcc gtggcgcata gcactgccag ctgaataaca catccccgtc
      181 aatacggccc tcgctgtacg cttacgcata aatattgaat acatttactg agcatgcaaa
      241 agaaaaagac aaactaatcc cggcctttca cagaatttcc tctatcagag gctgagttga
      301 caacctgctc acccaggaaa tagtaacacc cgaaaacatg aagagactct gataaacaat
      361 ctcgatacat attcctcatc tttcacagca tctcacttct ttaacaggaa ttattattat
      421 ctacgcctcc tcctttcatg tatccctcat gaatgattaa gataaacata ctgtgtttgt
      481 tatccgtatg ataacaaaca cataaaggag taaacattta tctggatgat gattacaatg
      541 ttcacgactg tcagagacac acctctgata cattagaaaa accaatgttt cccttaaaaa
      601 tggagcttat atgaataaaa tatactctct taaatacagc catattacag gagggttaat
      661 cgctgtttct gaattatccg gcagagtatc atcaagagca actggtaaga aaaaacacaa
      721 acgcatactt gcattatgtt ttttaggctt attacaatcc tcatattctt ttgcgtcaca
      781 gatggatatt tcaaatttct acatccgtga ctatatggat tttgcacaga acaagggcat
      841 atttcaggct ggcgcaacaa atattgaaat agtgaagaaa gatggctcca ccctgaaact
      901 accggaagta ccatttcctg acttctcacc ggttgcaaac aaagggtcaa ccacatctat
      961 tggtggtgca tacagtatca cagccacaca caatacgaaa aaccaccact cagttgcgac
     1021 gcaaaactgg gggaacagca cgtacaaaca aactgactgg aatacttcac atcctgattt
     1081 tgcagtatcc cgacttgaca agtttgttgt tgagacccga ggtgcgactg aaggcgcaga
     1141 tatttcgtta tcaaaacagc aggcacttga acgttacggg gttaattata aaggagaaaa
     1201 gaaacttatc gcattcagag ccggctctgg tgtggtatcc gttaaaaaaa atggacgcat
     1261 aactccattt aatgaggttt cttataagcc agaaatgtta aatggctctt tcgttcacat
     1321 tgatgactgg agtggatggc tgatattaac caacaaccag tttgatgagt ttaataacat
     1381 tgcctctcag ggtgacagcg gttcagcact gttcgtctat gataaccaaa agaaaaagtg
     1441 ggttgtcgct ggaactgtct gggggattta taattacgcc aatggcaaaa accacgcagc
     1501 atacagtaaa tggaaccaga caaccattga caacctgaag aacaagtatt cttacaacgt
     1561 ggatatgtca ggagctcagg ttgcaaccat tgaaaatgga aaactgacag gcactggctc
     1621 agacaccacc gatataaaaa ataaggactt aatatttact ggcggtggag atatcctcct
     1681 gaaatcctct tttgataatg gtgctggcgg tcttgtcttt aatgataaaa agacctatcg
     1741 agtaaacggg gatgatttca cctttaaagg tgccggtgtt gatacaagaa acggcagcac
     1801 cgttgagtgg aatatccggt atgataataa agacaacctt cacaaaattg gtgatggcac
     1861 attagatgtc cgaaaaaccc agaacaccaa cctgaaaaca ggtgagggtc ttgtcattct
     1921 tggagctgaa aaaacattca ataatatcta cataaccagt ggtgatggaa ctgtccgact
     1981 gaatgcagaa aatgcactgt ctggcggtga atacaacggt attttctttg cgaaaaatgg
     2041 cggaactctt gacctgaacg gatataatca gtctttcaat aaaattgctg caactgattc
     2101 aggtgctgta ataaccaata cgtcaaccaa aaaatccatt ttatccctga ataatactgc
     2161 tgactatatc tatcacggca acataaacgg gaatctggac gtacttcagc atcatgagac
     2221 gaaaaaagag aaccgtcgtc ttattcttga tgggggcgtg gacacaacaa atgatataag
     2281 cctgcgtaat acacaactgt ccatgcaggg acatgccact gaacatgcca tttatcggga
     2341 tggagctttc tcttgttcac taccagctcc tatgcgcttt ttgtgtggca gtgattatgt
     2401 tgcaggaatg caaaatacag aagctgatgc tgtaaaacaa aacggaaatg cctataaaac
     2461 caacaatgct gtctctgatt tatcgcagcc agactgggaa accggaacat tcagatttgg
     2521 aacgctacat cttgaaaatt ccgatttttc tgttggtcgt aatgcaaatg taatcgggga
     2581 cattcaggcc agtaaatcaa acattactat tggtgacact acagcatata ttgatttgca
     2641 tgctggtaaa aatattaccg gtgatggttt tggcttccgc cagaatattg tgcgtggaaa
     2701 ctcacaagga gaaacgctgt ttacaggagg gatcacagca gaagacagca ctatcgttat
     2761 taaagataaa gcaaaagcat tattttcaaa ttatgtatac ctgctgaaca caaaagcaac
     2821 catagagaac ggtgctgatg tgacaactca aagtggtatg ttctccacga gcgatatcag
     2881 catctctggt aatctgtcca tgacaggcaa tcccgacaaa gacaataaat tcgagccctc
     2941 aatatatctg aatgatgctt cttatctact gactgacgac tccgccagac tcgttgccaa
     3001 aaataaagca tctgtggtgg gagatataca ctccactaaa agtgcatcca tcatgtttgg
     3061 tcatgatgaa agcgacctct cgcagttgtc tgacagaacc tcaaaagggc ttgcacttgg
     3121 tcttttaggt ggctttgatg tctcatatcg cggttcagtc aatgccccgt cagcatctgc
     3181 cactatgaac aatacctggt ggcaactaac cggagattct gcgctgaaaa cactgaaaag
     3241 tacaaacagc atggtctatt tcactgacag cgcaaacaat aagaaattcc atacgctgac
     3301 ggtcgatgag ctggcaacca gcaacagcgc ctatgcgatg cgtacaaacc tttctgaatc
     3361 agacaaactg gaggtcaaaa aacacctgtc tggtgagaac aatattttac tcgttgattt
     3421 ccttcagaaa ccaacgcctg aaaaacaact gaatattgaa ctggtaagcg cgccaaaaga
     3481 caccaatgaa aatgtcttta aagccagcaa acaaaccatt ggtttcagtg atgtaacgcc
     3541 ggtcattaca accagggaaa ccgatgacaa aataacatgg tcactgacag gctataacac
     3601 ggtagcaaac aaggaagcaa cccggaatgc cgccgccctg ttctctgttg actataaagc
     3661 gtttctgaac gaggtcaaca acctgaacaa acgtatgggt gacctgcgtg atatcaacgg
     3721 cgaagccggt gcatgggcac gcatcatgag cggtaccggc tctgccagtg gtggtttcag
     3781 tgacaactac acgcacgttc aggtcggggt cgacaaaaaa cacgagctgg acggactgga
     3841 tttgtttacc ggtttcactg tcacacacac tgacagcagt gcctccgccg atgttttcag
     3901 tggtaaaacg aagtctgtgg gggctggcct gtatgcttcc gccatgtttg attccggtgc
     3961 ctatatcgac ctgattggca agtatgttca ccatgataat gagtacactg caacctttgc
     4021 cggactcgga acccgtgatt acagcacgca ttcatggtat gccggtgcag aagcgggcta
     4081 ccgctatcat gtcactgagg atgcctggat tgagccacag gctgagctgg tttacggttc
     4141 tgtatccggt aaacagtttg catggaagga ccagggaatg catctgtcca tgaaggacaa
     4201 ggactacaat ccgctgattg gccgaacggg tgtggatgtg ggtaaatcct tctctggtaa
     4261 ggactggaaa gtgacagccc gtgccggtct gggctaccag ttcgacctgc tggctaacgg
     4321 cgaaaccgta ttgcgggatg catctggtga aaaacgcatc aaaggtgaaa aggacagccg
     4381 tatgctgatg tccgttggcc tgaatgcaga aatcagggat aacgtccgct ttggactgga
     4441 gtttgagaaa tccgcctttg gtaagtacaa cgttgataat gcagtcaacg ctaatttccg
     4501 ttactcgttc tgatactccg tattcaaaag ggcctgcggg ccctttacgc tcagtgcctg
     4561 ccgccctgat atgtctgaga gacatttccg ggatgtcagt cgactggttt acttaactgc
     4621 gatgactaaa gctggcagta atgccggctt ttttacggcc acatacgtct tcctctcatc
     4681 cggaactgtg atacaaatcc tgcatacagg attgaccgcc ggttcctgca aatggcacat
     4741 tttcagtaag atagcgaagg ttacattccc ccgtcatgtg tggcacaagc tgtcaccggg
     4801 aaagaaacgg aaagcacatg aaaaacatca aaactcacac cggactcctt atccacaaag
     4861 aacaaacgcg gcgcgttcgt cttcatgaaa cacccactgc ctggtgccac acgcaccggg
     4921 aatgctacag caaaacgacc ggtcgccgct gcggcagccc tgacagtctc agcaggctga
     4981 ttctgagcag
//
LOCUS       DAZGOB010000220         4982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGOB010000220 DAZGOB010000000
VERSION     DAZGOB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.221"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..261
                     /locus_tag="ACHLIF_004883"
     CDS             <1..261
                     /locus_tag="ACHLIF_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO9608719.1"
                     /translation="ENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLI
                     AAGLESNLTVYTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
     gene            complement(403..1527)
                     /locus_tag="ACHLIF_004884"
     CDS             complement(403..1527)
                     /locus_tag="ACHLIF_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO9608720.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(2257..2454)
                     /locus_tag="ACHLIF_004885"
     CDS             complement(2257..2454)
                     /locus_tag="ACHLIF_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9608721.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(2520..2790)
                     /locus_tag="ACHLIF_004886"
                     /pseudo
     CDS             complement(2520..2790)
                     /locus_tag="ACHLIF_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3095..3283
                     /locus_tag="ACHLIF_004887"
     CDS             3095..3283
                     /locus_tag="ACHLIF_004887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608722.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            3611..3805
                     /locus_tag="ACHLIF_004888"
     CDS             3611..3805
                     /locus_tag="ACHLIF_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608723.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            4148..4276
                     /locus_tag="ACHLIF_004889"
     CDS             4148..4276
                     /locus_tag="ACHLIF_004889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608724.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(4586..4921)
                     /locus_tag="ACHLIF_004890"
     CDS             complement(4586..4921)
                     /locus_tag="ACHLIF_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608725.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1487 a         1130 c         1033 g         1332 t
ORIGIN      
        1 gagaataata cctcctgggt aagtgagcag atactttcag cgcctccgaa agatgtaaat
       61 attcgacttg gagtcggttc tttagaaggt acaaccgtct cacatgttca gcgattgcat
      121 cattcgttaa tcgcagctgg tttggaaagt aacctcactg tctataccgg tggtcacgat
      181 tatgcctggt ggcgcggagc aattattgat gaattagcaa attataattg caggaaggta
      241 tcaaataata actctgtgta aaatatcgtt ttatcgtcag ataactcttt ctgcaatatt
      301 attatgggag actatctcat ttatttagtg atgtccttca tttatgtgat gaaggacatt
      361 atattactca taacatttct cgttgaaaat ttcagcaata cgctacaatt gtttcccgtt
      421 gttttttaat tttctcatca aaacctctgc aatctctgca ggactgggtt gttcagccat
      481 ttcagcggct acctgatctg aacagaagcg tagtgaatca tcataaagaa ggcgatttac
      541 caaatcactg gtcagtccat gcttgtccgg aataatccca cacccacgct tcgcaacaat
      601 ttctgcatta acagagcgat ctgcaccttc gccaaacaca atctgtggta ttccactata
      661 cagcgctgtc agggtatttc ctgcgccacc gtgatgaata aatccatcag ctccattaag
      721 gaatacaccc ataggtatcc agtcaaccag acgtacattt gatggtagtt ttcgtaaacc
      781 agtacgagca tttattgcaa gttgcaaaat gatatcagca tcaacttcat ttgcagaatc
      841 cataacccat gaaatcagct ccagaccatc aaccattggt tttacagtgc caaggctgat
      901 aagtaaacgt ttccgatcag aatccctgtc ccaccatgtt tcctttacag cacctccgtt
      961 ataaggaata tatctcattg agataaccgg ttctccagca tttttaagaa tgctcatact
     1021 tgggggagca acatcaatcc atgctaaatc acatagtgga ccgctgactc catgccgcct
     1081 gtaagcattg cttaaagaac ggtttaacat ctgaatatgg gcagatgtat gcgcgaatcc
     1141 aacagccagc atcactgaag gaattctata tttagcagca accaatgggc ctgccggatc
     1201 aagaggggga tagactatta aatctggcct ccattttcct gcaaaatcga tgaggttatc
     1261 tgccatttca tcgctaaaaa aagagaaatg accaataata ttactttttt tcctcaactc
     1321 ttcctgatgg cgatatccag cctctgaatc taaacctgga actgcatcaa aaacaaccag
     1381 tccagcttct gctgctttat tcgcgaattt gccagcgcta ctaattacta catcatgtcc
     1441 aattacacga aaggcctgag ccagagaaat caatgggtat aacaaaccgt acagcggtgg
     1501 gccaataaaa agaatacgtt ttcgcattaa aaaagtccgt cttctgctta cctgtttatt
     1561 tttctatctc taagaaaaca taattaatat tatttttatg tgagcttatg cacaaaaata
     1621 accaagtata ttatcttctg gtagagattc aggatacagt ttgtaggagc tttctttttt
     1681 gaacaatgat ttgcaggaca gttatgctaa actagcggaa tcagtatcac aacaataaat
     1741 gactgatcat acctgaaatt tgaattaaca ggcatcatga atgtcaatag tctaagcggt
     1801 gactacttat atcgcttgca taaatactaa acactgacta gaaaagtatt ggataacccc
     1861 gtcactaaaa gtaacgcttc ggttatttgc tcggaatgta ttaacgccaa gattgttagc
     1921 cgttacgcct tatatcactg gcgctgacaa atgtttcttt tctaatttac tgaaccggcg
     1981 cagacacgca ggaaagcacg ccggcaccgg tgtaatgcca gttctaatca ataagaatgg
     2041 aatgacgaat cagtcaggaa gggataacaa cccgctgctg aacggaaggt atcagcagga
     2101 agatgaacgg gaatatcgcc agcaccaagg gcagggccgg gttccacaat accagcgctg
     2161 gcattttgca cattgccagc gtaccggcat tacaacacgt ttcatgctgc tgccccccag
     2221 gattcctcga ttcgttgcta catccactgc tctacttcac ttccactgct ccacttcact
     2281 tttgtaccag cggctgctgc gccccagttt gatgggttta gggaaatggc cttcactgat
     2341 caacttgtaa aaccatttgt cggtcatacc ggtgaaactg gtgatatagt tcatgtcgat
     2401 cagggaatta tcggcacgca gaaatacgct gtcgtggctt gttgtagtca acatggtcag
     2461 aatgtcctgt gagatttatg aagattgtgc gaatgcggga aatctactga gctgtgcttt
     2521 cagaactggc ctgttacggg ataccaggga ttaccggcgg ggtaacgggc ttccggatca
     2581 tacacaccac gattatcgcg gacaaaatca ctgaacgccc atatcacctc tttaagtatg
     2641 tcttcgcagt ccggtaaatg acgatccagc gccacatccc gggtggtaca gctttgatgc
     2701 gcccggtgac acaaagcccg gattgttcca gacatcctga atcaaacgcc ccagattagg
     2761 ggcgtcgaaa tatgcctctc tgaccattat attccggcgt ataggtagca ggtctgaagt
     2821 gacaatgcgt cacctgacgt taaaagtcac ccaagatgac cccagtgggg gcaacaagta
     2881 taaaaccgtt ttttgattaa gtggtcggat atgaaaaggg gaaggtttat gtgaaaaata
     2941 cctgtgtttt ctttgaaaac acacaacagt atcaggttat ttatgtttat acagtgcgtt
     3001 ccaaactgac ctgaataatt acgcaagttg caacaataaa acatttttgc cgtagtgtga
     3061 ggtactccgg taaattgctt cccaggtgca gacaatgact aacgaagaac tacaggcaat
     3121 cgcattatcc atgcttgagc gccagcgcga aaaagtacac cagcaacgag agtctttgct
     3181 taacccatct ccattcacca ggccaaaacg attggagctt gaagcattct tggacaaata
     3241 cccacggcgg cttaaatcag gcaaaaccag accaccaggc tgatagctca cagtttgttc
     3301 tccgattcaa taaaacggat aagaaccatc agcgcggtag cgtaaggcac tacagcgcat
     3361 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
     3421 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
     3481 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
     3541 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
     3601 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
     3661 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
     3721 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
     3781 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
     3841 gcatcgttat cagcgcactt gattttcttt atatccactg acgagtttgc cggtcattct
     3901 ggtatcatgc agatatttac ggcgtacaag cacactagca tgacattgta ataaggcctg
     3961 agcctaacgc tttaatgtgt gtcatacact cagtggcgta atcgttgaca atatggtgca
     4021 tgtaatcgta agcgtccagc gaaatccatc tggtcataat ctgaagcatc cgacaaagtg
     4081 gtgtccacca aataagtgat ctgcgcccaa tatcctgagc cggaactttg acaaaggcca
     4141 acgaaaaatg ggagcggatg ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg
     4201 ccagttctcc gatctgttta accgggaaat tatctcctac agcttgtcag aaaggtcggt
     4261 gatggagcat cgttaatacg atgctatacg atgcattcac agtgcccggc cagaggatgc
     4321 cccgtcgctg catatggatc agggttggca atatcgaatt gcaggctata ggcaaagtta
     4381 aagccccatg gagtagcaca aaatatgccg cacaaaggaa acggtctgaa taacgcagtg
     4441 atgaagaact tcttcagcac actgctaaaa cgcatagtga tcgagcgctg attctggcga
     4501 acaactgaac taatacatca gaatctgcat tatgttaaat aaatataaaa agatggttta
     4561 aataccccgt tacttttgac ttacactata cggtatcgca tcgtttaata ttcgcaccgg
     4621 ccagattttt atttctatta gttgtcacaa tactgaatgc gtacgaccac agtattctgg
     4681 ctcctgtgtg gttatgcttt aattctgcgt tccgggcaga taagcagttg cttgcaggaa
     4741 tccttcttgt gttaatgtca gttccccttt taccagtgct gatttccaca ttccgtccaa
     4801 cagagcttat agcctttccc tggattatag cattgtccgg ctgaagttct ttttgaataa
     4861 taatagaagc actgctggca gatccagtcc gtttttcata acccactgta ctgataacca
     4921 taatctaatc agtagaaatt gagtcgaaaa taagcactac tccatacagg ataattagag
     4981 gt
//
LOCUS       DAZGOB010000221         4972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGOB010000221 DAZGOB010000000
VERSION     DAZGOB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.222"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..874)
                     /gene="chbG"
                     /locus_tag="ACHLIF_004891"
     CDS             complement(116..874)
                     /gene="chbG"
                     /locus_tag="ACHLIF_004891"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO9608726.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(887..2239)
                     /gene="celF"
                     /locus_tag="ACHLIF_004892"
                     /gene_synonym="chbF"
     CDS             complement(887..2239)
                     /gene="celF"
                     /locus_tag="ACHLIF_004892"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO9608727.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(2344..3186)
                     /gene="chbR"
                     /locus_tag="ACHLIF_004893"
     CDS             complement(2344..3186)
                     /gene="chbR"
                     /locus_tag="ACHLIF_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO9608728.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(3194..3544)
                     /gene="chbA"
                     /locus_tag="ACHLIF_004894"
     CDS             complement(3194..3544)
                     /gene="chbA"
                     /locus_tag="ACHLIF_004894"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO9608729.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEVKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(3595..4954)
                     /gene="chbC"
                     /locus_tag="ACHLIF_004895"
                     /pseudo
     CDS             complement(3595..4954)
                     /gene="chbC"
                     /locus_tag="ACHLIF_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
BASE COUNT         1361 a         1310 c         1056 g         1245 t
ORIGIN      
        1 agcgcatcag gcagttttgc gtttgtcagc agtctcaagc ggcgcagtta cgccgccttt
       61 gtaggaatta atcgccggat gcaaggttca cgccgcatct ggcaaacatc ctcacttaca
      121 catcccgata actccccaac cgataaccac gctgagcgat agcacctttc aacgacgctg
      181 atgtcaacac atccagctcc gttaagcgtg ggaaacagta agcactctga cggatagtat
      241 tatcgataaa cgccggatga cacatcacct ccagcgaacg atcaccccga tggcttgcgt
      301 catccagcac ttgcaggaac agtgcctcgc taatctcttc accataaaac gcgctactga
      361 atccctgaga actgcgcaga ttagccggta aatcaccagc gttagatagc ggctgacggt
      421 cgatgcgcaa cgcaatcccc tcttcagccg caaacctggc aacaatcggg aaaatctgcg
      481 ggaacatatg cacatgatga tggctatcaa gatgcgtagg tttgcgtcca aatagctcaa
      541 tgaaacgcaa atactgactg gcaagctcct gagtaatttc ttccagcggt aaagcatctt
      601 cttctgccaa ctgccagatc cattttccca gcacaccatc gcgggttaac cccggcatag
      661 ctgtcagggg cttgcccata gtaaggacaa agtgcatccc tatggccaga ctcggttcat
      721 cacgactcaa ctgcaccgca tggtcaatag cctgcccatt caccagcgcc gtcgttgatg
      781 tgacaatccc attgcgacag gcctcgataa tgccgtagtt ctgacctttg cttaagccaa
      841 aatcatcggc attaacaatc agtaagcgtt ccataatcag cctcggttaa tgtgcttttt
      901 taagctctgc gatgcagtcg gcaaaatttg gcagccattt ctcgtgcgcc agaatcatct
      961 cgcgtgccag cagctcagca tcgcgatcgg aatgcaccaa cggactaagg tttagcgcca
     1021 gtaacacatc gttaaattct ccgctaagtg cggcgttgct ggcagcaatc tcgaagcctt
     1081 taatggtgtg aatcagcccc atcactttat catcgaaatg cgtaatgcgc ggatgtggcg
     1141 tcgcgccatc gcgccccagc ttacaggtca tttctaccgc ccagtctgcc ggaatattat
     1201 caatatgccc atgatgcggg atattaacgt aatgttcagc ttgcttgtcg ttgtagatag
     1261 cgttgatcac ttcgcacgct gcatcagagt aataagcccc accgcgctgt tccagttctt
     1321 tcggcttaac tttcaactcc ggatttttat acagctcaaa aagttgtttc tcgactttct
     1381 gtactacctg tgctcgtgcg ccgcctttgt agtattcgcc catttcaata gccagcatct
     1441 ctttctgctt gaagtaatac agcagataag aacatggcag cagatttaac gaacgaatta
     1501 agccctcact aaatggcaga tcgaaaatat tttttacaga ggacgctttt aactgccctg
     1561 acgccacacc atcaagcaat tcggcaaagc gcgacttgcc atttaccagc acatccttaa
     1621 tgaacaccat atggttgagg ccgaacagat cgatagataa atcatcgctg tctttcagca
     1681 tcagaacatc gcgaataaac atcttcatgc cgatcggaat attacacacg ccgataaagc
     1741 gtttaaatcc ggtatgacga taaacggctt cagtgaccat tcccgccggg ttagtgaagt
     1801 taatcaccca tgcattcgga caaagttctt cgacatcttt tacgatgtca aaaatcaccg
     1861 gaatggtacg cagaccttta aacagaccgc ccgcgccgtt ggtttcctga ccaagataac
     1921 catgacttaa tggaatacgt tcatccagtt cgcgcgccgg taattggcct acgcgcagtt
     1981 gggtagtaac gaaatcagca tctttcaatg cttcgcggcg atccagcgtt ttataaagct
     2041 tcatcgggac gccagcgtta tcaatcatcc gttggcagag ttcaaaaatg atatccagtt
     2101 tctctttacc atcttcgaca tccaccagcc ataattcgct gaccggcaat tcgtgataac
     2161 gcttaataaa tccttccagt aactccgggg tatagctgct cccgccacca atagtgacga
     2221 cttttaattt ctggctcata atttctccct tcagtacaga atactgatat ctggcatatc
     2281 tgcccccccg gacataaata atccagcaac aggacagata tgtgaattgt caggtataac
     2341 gacttactga ttaaattcag tcaatttctt acgatagctc ttaggcgtaa aggacgttaa
     2401 tttcttaaac gttttaataa acaaactcgg gctactataa ccggcctcaa acgcaatatc
     2461 cgtcacggaa tagttggtca tttccagttg ttttttggca aaattaatac ggatctcatt
     2521 aataatctgc attggtgttt tgccataata tcgttgagtg gctcgcgtca aatattcctg
     2581 tgatttggct gacaacgtca ccatattctc cagcgccgat tcactaaact gctctttatc
     2641 atgcatcttt tctaccgtac ttttcagcca ctgcggtaca tcatcaatca cctgttcttc
     2701 gcggtaatga cgtaaacggt taatgacata aaaagtaacc atctcaacaa actcttccag
     2761 ccctgtttcg cggaaattca atgaagaaat cactgtttcc acataggtga gaaaagcgtt
     2821 attggtccgg tatacctgcg aagcgacaaa gcaataaggc aacaacggca ggtaatgctg
     2881 ctcaaaaaag cgtttactga tcccaacgtt caatatacgc gtggcaccaa actcataaaa
     2941 actttgatgg tgcgaaccta acggaataaa aacaaaatcg ccccgttcca gtaacacgcg
     3001 cttaccgtta atctcctgga aataacgccc ggttaatacc agagtaaatt cataatagtc
     3061 gtgctgatgc agcccgctga tactctcagt tttgttatag ataaacacat ggaagttttt
     3121 gccattaaac aactgctgtt ctcgggcagt ggcaatttcc ggcgcgttaa tcactggctg
     3181 catcatcgac tccttatgcc ttcagttttt catgaagctc aattaattca gtaatcagtt
     3241 cacgcgcaag catggacgtc attaaatgat cctgagcgtg gaccagcacc agactcactt
     3301 tcatcttaac ttcgcccgca tcgccttcaa tcagtttcgt ctgtaccaga tgcgcttcat
     3361 tcaatgccat ccgtgactga tccatcatgg cttttgctgc agcaaaatcg ccctgcttcg
     3421 cctgtttcag tgccgcatac gccaggctgc gcgcttgtcc ggagttgatg ataagcccca
     3481 tcaccacttc ttccagctct tcagcttctg tttgcgtatc aggaatatta tcgagatcca
     3541 tcatacatcg ttcctctctc ttcttaccgg caagattacc cgtaccggca tcgattaaaa
     3601 tttcagggcg ttagcgatat cttcttcgct ctcttcttta tcaatcgcat tttgtgcttt
     3661 gttagccacc acaacaaagg gcagataaat taaggttgcg atgccaaggt tgaagagtgc
     3721 gaccagcaat gcggcgacgc taccgttggt gttaaagaag gctcccagac cggttggcat
     3781 ggtccacggt gcaatattgg tcaccggagg aatgatgccc atgtagtacg ctgcgagggt
     3841 gattgccgcc agaatcggtt gtaccagtac aaacgggata aacatcaccg ggttcatgat
     3901 aattggcaga ccaaacagaa tcggttcgtt aatctggaag atgccggacg gcagcgccag
     3961 ttttgccacc tgacgataat cagcacggcg agaggcgata aagatagcca ggatcaggcc
     4021 taaagtcgca ccactgcccc caaggaaaat aaaggagtcc agcatcggct tggcccagat
     4081 atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa ccatattgct gataggtcgc
     4141 gatattttcc agcgcccacg gcgtcataat gccgttgtcc agtgcggtca gcgccagcgc
     4201 gccatgaata ccgaagaacc agagcagtgg aacaaagatc acataggccc agcccaccac
     4261 gctacccaac gatgccagtg gggttgagat ggtatccata atgatctgat ggaagttggt
     4321 gccccaggta ttcaacgccc aggcaataat ccccatcacg gaaagaataa taaagccggg
     4381 aattaatgcc gagaaggaac gcgatactga agcaggtacg ctgtcgggca gtttaatgac
     4441 ccaattgcgg cggacaataa aggtaaacat ttctgccacc accaggccaa taataatccc
     4501 ggagatgata ttcgccccac ctaaccagtt tgcgccaacc gcataggcct caccgacact
     4561 atatgggggt gacggtcata aatgctgcaa cggataacaa ccccgccgcc agcgcatcga
     4621 ctttacgctc ttctgccagc gccatgccaa taaagaacgg tgccattaaa gacattatcc
     4681 ctaatgttcc gttatatacg ttgccgccaa tacctttcag gccattaagt gtttcaatgg
     4741 ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga cccctcccca aagcttagaa
     4801 aaacgttgtt aattaataca aacatggccc ccgcaagggt taacggcatt aagcgaataa
     4861 agccattttt gattgcatta acgtgtggct gctttcctat tttaactgca aaagggagga
     4921 gtaccttttc aagcgatgca ataacattac tcatagaaaa atacccttaa aa
//
LOCUS       DAZGOB010000222         4905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGOB010000222 DAZGOB010000000
VERSION     DAZGOB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.223"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(52..813)
                     /gene="kduD"
                     /locus_tag="ACHLIF_004896"
     CDS             complement(52..813)
                     /gene="kduD"
                     /locus_tag="ACHLIF_004896"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO9608730.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(843..1679)
                     /gene="kduI"
                     /locus_tag="ACHLIF_004897"
     CDS             complement(843..1679)
                     /gene="kduI"
                     /locus_tag="ACHLIF_004897"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO9608731.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1966..3147)
                     /gene="yqeF"
                     /locus_tag="ACHLIF_004898"
     CDS             complement(1966..3147)
                     /gene="yqeF"
                     /locus_tag="ACHLIF_004898"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO9608732.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3402..4631
                     /gene="yqeG"
                     /locus_tag="ACHLIF_004899"
     CDS             3402..4631
                     /gene="yqeG"
                     /locus_tag="ACHLIF_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9608733.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1289 a         1254 c         1065 g         1297 t
ORIGIN      
        1 ggtataaaaa accctgccat gcggcagggt cataaaagta agaagaatga attaacgcgc
       61 cagccaaccg ccatccacgg caatggtata accattcaca taatctgaag cgctggaggc
      121 aaggaacact atcggcccca tcaggtcact cggcagtccc caacgaccag ctggaatgcg
      181 gtcgagaatt tccgcgctac gttgttcatc tgcccgtagt tgttgagtat tgttggtcgc
      241 catgtaaccc ggagctatcg cattaacatt aatgttgtgt ttagcccatt cgttcgccat
      301 caatcgcgtc acacccatca cgccgctttt tgatgcggta taagaaggca cacggatccc
      361 gccctggaag gagagcattg acgcgatatt gataatcttg ccgccattgc cttgcgcgat
      421 aaagtgtttc gccgctgcct gagacatgaa gaatacgctc ttgatattca ggttcatgac
      481 atcgtcccag tccttttcgc tgaactcgag agcatcttcg cggcgaatca atccggcgtt
      541 attcaccagg atatcaatat gaccaaactc cgctaccgcg cgatccagca gtccaggaat
      601 gccatcaatc tttcgcagat cggcggtcag gcttaaaaaa cgacgcccca gcgccgtgac
      661 ctgctcgatg gtttcagtcg gttcaacgat gttaatgcca acaatgtcac agcccgcttg
      721 cgccagcccc aacgccatcc cctggcccag cccagtatca caaccagtga cgaccgcaac
      781 tttaccttcg agagaaaatg cacttaaaat cataacaata ccttattcgt ttatgcccac
      841 aactagcgca aatctttaac ggccacatgg tccatatcat caaagacctg gttttcaccg
      901 accatgcccc agataaaggt ataagctttg gttccgacgc cggaatgaat cgaccagctc
      961 ggggagatca ccgcctgctc gttatgcatc acaatgtgac gcgtttcttg cggctgcccc
     1021 atcatgtgga aaacgcaggc gtcatcatcc atattgaaat agaaataaac ttccatccgg
     1081 cgctcgtgag tgtgacacgg catggtgttc cacaagttac ctggagccag ctccgtcagc
     1141 cccatactca attggcaggt ttccagcaca tccgggacaa aatatttgtt aattgtgcga
     1201 cggttactgg tgaggttatc gcctaacgtg actggagata cttcgtccgg tgtgactttt
     1261 ttggtgggat acgtcgtatg cgcgggtgcg caattgtaat aaaactttgc cggagtggcg
     1321 gtatcaatac tggcaaagac aacttctttt gcacctttac ccacatacag ggcgtcgcgg
     1381 tgaccgattt catagcattg gccatcgaca gtaatcgtac cggcaccgcc aatattgata
     1441 acacctaact cgcgacgttc aaggaaatag cttacgccga gttgtttacc aacttccccg
     1501 ccaacggaaa ccgttttagt tatcggcata atgccgccaa caataattcg gtcaatgtgg
     1561 ctgtaaacca tggtgtactc atcggcgaca aatacctctt caaccaaaaa ttcattgcgc
     1621 agcccttggg tatccagcgt ttttgcgtgc gcactgtgga tgctctgtct tacgtccaca
     1681 tcaacctccg ataatcagtg aagcgtgaaa atagaaaacg atagtgaaac actgtttcgt
     1741 tttctctata aagggatgat atgcccatgg gctagggttt tcaattaaaa ataaaacaat
     1801 gtttcatttt taagttaaga attaaaaaaa gtgcagtgtc gatcacgaat atgtcatgag
     1861 aatggaaaaa tgagggagcc cagaaaatgt atgtcgactt gccagccatc attattcaac
     1921 agattaatga cggcagatat ttatcggtaa ggaggaaatg cagagctact cgtcacgttc
     1981 aatggtcaat gccacgccct ggcccccgcc gatacaaagc gttgccagcc ctttgcgggc
     2041 attacgtttc accatttcat gaaccagaga aaccaggatt cggcaaccgg aagcgcctat
     2101 cgggtgaccg agtgcgatcg cgccaccatt gacattgacc cgacgctcat cccactcaag
     2161 catcttgcca accgaaagcg cctgtgcagc aaacgcttca ttagcctcga taagatcgac
     2221 atcagccaac tgccagccta cacgctccag gcaacggcgg gtcgcataca ccggcgcaat
     2281 tcccatcaat gccggatcta caccaacgct ggcaaatgcg cggatgcggg ccagcacggg
     2341 taaattcaac gctcgtgctt tggcttcgct catcatcatt accgcagctg cgccatcgtt
     2401 tatggatgat gcattccccg cggtcacaga accgagacta tcaaatgaag gatttaaacg
     2461 ggctaaacct tctgcactgg cgtcagtgcg tggctgttca tcggtatcaa caaccaacgt
     2521 ctgaccgtta ctttggatca ttaccgggac gatctcatct ttaaatcgtc cggcgtcaat
     2581 cgccgctcgc gctttttgtt gcgagctaag tgcgtaagca tcctgcaact gacggctgat
     2641 gccatattcg cgagccagat tttcggcggt gacaccaata tgataatcat tgaaggcatc
     2701 ccacaaccca tcatgcacca gactgtcaac caactggcta ttgccaagct gtgcaccggt
     2761 gcggctatca gtcagaacat gtggtgcgcg gctcatgttt tcctggccac cggcgatgac
     2821 aatatcagcc tcgccacact gtatcgcctg agtagccaga tgcagtgctt taagcccaga
     2881 accgcaaacg tcattaatag tgattgcaga aacgctatta ggaagaccac ctttaatagc
     2941 cgattgcctt gccggattct gccctgcccc tgcagtcaac acctgaccaa gaattacttc
     3001 atccaccgca tatgcaggaa cgccggtacg ttctattaac gctttcacga ccagactacc
     3061 aagttccacg gcggaatgac ccgctaacgc accgcgaaag cagccgatag gtgtccgtaa
     3121 cgccccgaca atcacaacgt ctttcatcac aacctcgcac caattaacag tgcaatagta
     3181 aatgattgtt aattacagtt atcttaattg tttaaaaaaa gtgattttta tcacaaagga
     3241 aatatgcctg agcagtagtc agagtcataa ctggcacgta aggtttgcaa ccactaaccc
     3301 accaatagag gggtagatag ggcgttaatc tcccatactt aacctggttt atggtaaatt
     3361 gccctccatt ttgtttaatt tgtagatgat acgttcagat aatgtctaat atttggtcta
     3421 aagaagaaac tctgtggagt ttcgcgctct acggcacagc cgttggtgca ggcacgctct
     3481 tccttcctat tcagttaggt tcggcagggg ctgtggtcct gtttattact gctctggtcg
     3541 cctggccttt aacatattgg ccacataaag ccttatgcca gttcatcctc tcatcgaaaa
     3601 catcagcagg tgaagggata acgggcgcag taacacacta ctatggcaag aagattggta
     3661 atctgattac cacgctgtac ttcatcgcct tttttgtcgt cgtgttgata tatgcagtgg
     3721 caattaccaa ctcacttacg gaacagctgg caaagcatat ggttattgat cttcgcatcc
     3781 gtatgttggt gagtctgggt gttgtattaa tcctgaatct catttttctg atgggacgcc
     3841 atgcaaccat tcgggtaatg ggttttttgg tattcccatt gattgcctat ttcttatttc
     3901 tttccattta ccttgtcggt agttggcaac ctgatctatt aacgacccag gtagagttca
     3961 atcagaatac ccttcaccag atatggatat cgattcccgt gatggttttc gcctttagcc
     4021 atacgcccat tatttctacg tttgccatag acagacgtga aaaatatggc gaacacgcta
     4081 tggataaatg caaaaaaatt atgaaagtcg cttatctcat catctgcata agtgtactgt
     4141 tctttgtctt tagctgcctg ctttctattc caccttcgta tattgaagcg gctaaagaag
     4201 aaggggtcac cattttatcg gcgctttcta tgctgccgaa cgccccagca tggctgtcaa
     4261 tttccgggat tattgtcgca gtagttgcga tgtcgaaatc attcctgggt acgtactttg
     4321 gcgttattga aggtgccacg gaggtcgtca aaacaacact acagcaggtt ggtgtaaaga
     4381 aaagtcgtgc atttaaccgc gcactatcaa ttatgttggt atcgctgatc accttcattg
     4441 tttgttgcat taacccgaac gcgatttcga tgatttacgc gatcagcggc ccgctcattg
     4501 ccatgatact tttcatcatg cctacgttgt caacgtatct gatcccggcg cttaaaccct
     4561 ggcgttccat cggaaatctg attacgctga tcgtgggtat cctgtgcgta tcggtaatgt
     4621 tctttagctg atatccattg tgaatggttc aaaattgcta caattttgaa ccattccagt
     4681 tttcataatg ttcattcgtt attattccgc actatcactt ccctcatcat atcctagtat
     4741 tatcagttag ttaaacacac aaagagatct gaacatctta aataagacta tttaagatgc
     4801 ataacttaga ttcgcaagat atcttccggt cattcaaaaa caatttgcga tatagatatt
     4861 ctcataccat gagatagtta tctgaaccac cctcaaccag gtaag
//
LOCUS       DAZGOB010000223         4880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGOB010000223 DAZGOB010000000
VERSION     DAZGOB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.224"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..435
                     /gene="cesT"
                     /locus_tag="ACHLIF_004900"
     CDS             <1..435
                     /gene="cesT"
                     /locus_tag="ACHLIF_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFO9608734.1"
                     /translation="AEKIGIGSISFNENRLCSFAIDEIYYISLSDANDEYMMIYGVCG
                     KFPTDNPNFALEILNANLWFAENGGPYLCYESGAQSLLLALRFPLDDATPEKLENEIE
                     VVVKSMENLYLVLHNQGITLENEHMKIEEISSSDNKHYYAGR"
     gene            496..3315
                     /gene="eae"
                     /locus_tag="ACHLIF_004901"
     CDS             496..3315
                     /gene="eae"
                     /locus_tag="ACHLIF_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type beta"
                     /protein_id="HFO9608735.1"
                     /translation="MITHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENY
                     FKLSSDSKLLTQNAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMM
                     KAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHGGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTAD
                     KTSAKADGIEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATVTL
                     KSATPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITYTV
                     RVMKEGAPVVDQKVTFSKDFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSGVG
                     TEVKATTVEFFAPLSIDGDKVTVIGTGITGALPKNWLQYGQVKLQATGGNGKYTWKSS
                     NTKIASVDNSGVITLNEKGSATITVVSGDNQSATYTINAPGSIVIAVDKNTRVTYFDA
                     ENKCKTNSANLAQSKELLANIYSTWGAANKYPYYSGSKSLTAWIKQSSSEQSSGVSST
                     YDLVTKNQLINVGVNNKNAFSVCVK"
     gene            complement(3530..4750)
                     /gene="escD"
                     /locus_tag="ACHLIF_004902"
     CDS             complement(3530..4750)
                     /gene="escD"
                     /locus_tag="ACHLIF_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO9608736.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
BASE COUNT         1458 a          903 c         1062 g         1457 t
ORIGIN      
        1 gcagaaaaaa ttggtattgg atctatttca tttaatgaaa atagattgtg ttcttttgct
       61 attgatgaga tttattatat ttcgttatct gatgctaatg acgaatatat gatgatctat
      121 ggcgtctgtg ggaaattccc gacagataat cctaacttcg ctcttgagat tttgaatgca
      181 aacttatggt ttgcagagaa tggtggacca tacctgtgtt atgaatcagg agcacaatcg
      241 ctgttgttag cgttacgttt tccgctcgat gatgctacac ctgaaaaact tgagaatgaa
      301 atagaagtcg tcgttaagtc aatggaaaac ctgtatttgg tattacataa tcagggaata
      361 acattagaaa acgaacatat gaaaatagag gaaattagtt caagcgataa taaacattat
      421 tacgccggaa gataaaagat tatttattaa tataatttac tattcattct aactcattgt
      481 ggtggagccc ataacatgat tactcatggt ttttatgccc ggacccggca caagcataag
      541 ctaaaaaaaa catttattat gcttagtgct ggtttaggat tgttttttta tgttaaccag
      601 aattcatttg caaatggtga aaattatttt aaattgagtt cagattcaaa actgttaact
      661 caaaatgccg ctcaggatcg ccttttttat acgttaaaaa caggtgaaac tgttgccaat
      721 atttctaaat cacagggtat cagtttatcg gtaatttggt cactgaataa acatttatac
      781 agttccgaaa gcgaaatgat gaaggctgga cctggtcagc agatcatttt gccactcaaa
      841 aaactgtctg ttgaatatag tgccttacct gtcttaggtt cggcacctgt tgttgctgca
      901 ggtggtgtcg ctggtcatac gaataaaatg actaaaatgt ccccggacgc gactaaaagc
      961 aacacgaccg atgacaaggc tctaaattat gcggcacaac aggccgcgag ccttggtagc
     1021 cagctccagt cgcgctcact gaacggcgat tacgcgaaag ataccgctct tggtatggcc
     1081 agcagccagg cttcgtcaca gttgcaggcc tggttacaac attatggaac ggcagaggtt
     1141 aatctgcaga gtggtaataa ctttgacggt agttcactgg acttcttatt accgttctat
     1201 gattccgaaa acatgctggc atttggtcag gtcggtgcgc gttacattga ctcccgcttt
     1261 acggcaaatt taggtgctgg ccagcgtttt ttccttcctg aaaatatgtt gggctataac
     1321 gtcttcattg atcaggattt ttctggtgat aatacccgtt taggtattgg tggcgaatac
     1381 tggcgagact atttcaaaag tagcgttaac ggctatttcc gcatgagcgg ctggcatgag
     1441 tcatacaata agaaagacta tgatgagcgc ccggcaaatg gttttgatat ccgctttaat
     1501 ggctatttac catcatatcc ggcattaggc gccaaactga tgtacgaaca gtattatggt
     1561 gataatgttg ctttgtttaa ttccgataag ttgcagtcga atcctggcgc ggcgaccgtt
     1621 ggtgtaaact acactccgat tcctctggtg acgatgggga tcgattaccg tcatggtacg
     1681 ggtaatgaaa atgatctcct ttactcaatg cagttccgtt atcagtttga taaaccgtgg
     1741 tctcagcaaa tcgagccaca gtatgttaac gagttaagaa cattatcggg cagccgttac
     1801 gatctggttc agcgtaataa caatattatt ctggagtaca aaaagcagga tattctttct
     1861 ctgaatattc cgcatgatat taatggtact gaacacagta cgcagaagat tcaattgatc
     1921 gttaagagca aatacggtct ggatcgtatc gtctgggatg atagcgcatt acgcagtcag
     1981 ggcggtcaga ttcagcatgg cggaagccaa agcgcacaag actaccaggc tattttgcct
     2041 gcttatgtgc aaggcggcag caatatttat aaagtgaccg ctcgcgccta tgaccgaaat
     2101 ggtaatagtt ctaataatgt acagctcact attaccgttt taccgaatgg gcaggttgtg
     2161 gaccaggttg gggtaacgga ctttacggct gataaaacat cggctaaagc ggatggcata
     2221 gaagctatta cctataccgc gacggttaaa aagaatggtg tagctcaggc taatgtccct
     2281 gtaacattta gtattgtatc cgggactgca actcttgggg caaatagtgc cagaacggat
     2341 ggtaacggta aggcgaccgt aacgctgaag tcggctacgc caggacaggt cgtcgtgtct
     2401 gctaaaaccg cggagatgac ttcgccactt aatgccagcg cggttatatt tgttgatcaa
     2461 accaaggcca gtattactga gattaaggct gataaaacaa cagcgaaggc agatggttct
     2521 gatgcgatta cctatactgt cagagtgatg aaggaggggg cacccgtagt agatcagaaa
     2581 gtgacctttt ctaaggattt tgggaccctg aataagactg aagcaacaac cgatcagaat
     2641 ggttatgcta ctgtaaaatt atcatccaat actcctggca aggccattgt tagtgcaaaa
     2701 gtgagtggag taggtacaga agttaaggct actaccgttg agttttttgc cccgttgagt
     2761 attgatggtg ataaagtgac cgtaattggt actggtatca cgggggctct gccaaagaac
     2821 tggttacagt atggtcaggt taagctacag gcaacagggg gcaatggaaa atacacatgg
     2881 aaatccagta atactaaaat tgcttctgtt gataactcgg gagtgataac cttaaatgaa
     2941 aaagggagtg ccacaattac tgtagtatct ggtgataatc agagtgcgac atacacaatt
     3001 aatgcaccgg gtagtattgt aattgctgtg gataaaaata ctcgagttac gtattttgat
     3061 gccgaaaaca aatgtaagac aaatagcgca aatttagcac agtcaaaaga actattggcc
     3121 aatatctatt caacatgggg tgctgcaaat aaatatcctt actattctgg ttctaaatca
     3181 ttgactgctt ggattaaaca atcctcttct gaacagtcat caggtgtatc aagcacatat
     3241 gatttggtta cgaagaacca gttgatcaat gttggagtaa acaataagaa tgctttttct
     3301 gtttgtgtaa aataatttaa ttacatctca aatcccggaa aataaaattc attttccggg
     3361 attttttatt gtacctactt gaatctttta ataaacgttt catgttaaaa tcaatgtctc
     3421 atttcggaat aagtttttcg tgtatcttgt ttgatagaga ccttgtttac catatctaca
     3481 acatctgcac aataaaaaac cctccgaaga gggggaagat aataataagt taataagaca
     3541 acggaatatg tatatattcg tctgggcggg agatgttgat accatcttta ggatcaatcg
     3601 cgtctatgac atagcctcct ttcagaatgc taccaattcc caggcttgta ccgttatcaa
     3661 gagcaattgt tggattcttc atcgtgccgc tgattctgag tatttttccg gggaaaatat
     3721 ctgctgaaat gtacggctga atgttctgaa atagtatctt gacgttacta tttttgttca
     3781 tcgcatttat aatggcaagg atcttactct cgttctgttg agaaacctcc cccgcaacaa
     3841 tgatgacatc attctttttg aaaatattga catagtttat taatttattc tttttgaact
     3901 ctgatgccag ttcattgatt ttattgttac tgttgttatg tatttcccat ccagcaaccc
     3961 ctggcgtatt cagtaacaga ttttcaactt cgctccattt tggtgaaggg ggaatgtatc
     4021 ccgataacag gaagaatcca ggcttattac cttttgtaat gatgatatcc ttataaccgt
     4081 attccgttaa aatatcatta atcgcggaga tgatttggtt attgcaaatg atattgttgt
     4141 aataaaaaat atttttttct ttcaggtaat taaaaaatgg agttaaatca gtggttgatt
     4201 cgcaacgccc ataaagcgcg acatttttcc catgccatac cagagtgatt gcatgaagtt
     4261 tgttttcttt tagctgctgg tatacatcag ctctggtaaa cgctgggggt tcagcttttg
     4321 gggaaagcaa aacgtaactg ccgatggcgc cagacaacgc aatcgcagca ccaatcacgc
     4381 tagccaggat aagacgcttt ttacgtcgat ttttggcaga gaacctttct ggcacatcag
     4441 acaatggcaa ggtgtggtca atatcaccaa tgatgaagca acatcccgct atatcgatga
     4501 cctttccgac aggtaatgac tgatctgctt catagggggt gggctgtcca tcaatccaaa
     4561 attcaaccgg agataaaagg aaaacacctt cctcacgtat atcaagtaaa aactgattga
     4621 ggccttgttc aaggggaaaa taaacaatat cgttatcttc tgtgcctatc gttaggtttc
     4681 ccatcggaag ttgtaattcc cgattacgca tcgcaccatt gagaagtttt attttatatg
     4741 aggataacat ttttaaattt tattcatcct ggtggttgaa caatgagaaa aaatatggtg
     4801 aacttacatc aactaagaat agagtagaaa ggaagctgtt gtacagtact atttaatgga
     4861 atattcataa ttaatgatta
//
LOCUS       DAZGOB010000224         4869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGOB010000224 DAZGOB010000000
VERSION     DAZGOB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.225"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            78..1994
                     /gene="mngA"
                     /locus_tag="ACHLIF_004903"
     CDS             78..1994
                     /gene="mngA"
                     /locus_tag="ACHLIF_004903"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO9608737.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLGSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            2012..4645
                     /gene="mngB"
                     /locus_tag="ACHLIF_004904"
     CDS             2012..4645
                     /gene="mngB"
                     /locus_tag="ACHLIF_004904"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO9608738.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            4670..>4869
                     /locus_tag="ACHLIF_004905"
     CDS             4670..>4869
                     /locus_tag="ACHLIF_004905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608739.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKI"
BASE COUNT         1188 a         1143 c         1346 g         1192 t
ORIGIN      
        1 gtgaaattga tcacataatg gtattgtttt atcgggcaca ctggcgcgac tataaaaacg
       61 atcaagtgag gatcatgatg aatctgacga ctctgaccca ccgcgatgcg ttgtgtctga
      121 atgcgcgctt taccagccgt gaagaggcca tccacgcgtt gactcaacgt cttgctgctc
      181 tggggaaaat ttccagtact gagcaatttc tggaagaagt gtatcgccgt gaaagccttg
      241 gcccgaccgc cttaggtgaa gggttggctg tgccgcatgg caaaactgct gcggtaaaag
      301 aagcggcgtt tgcggtcgcc acactcagcg agccgcttca gtgggaaggc gttgatggcc
      361 cggaagcggt tgatttagtg gtgctgctgg cgattccccc caatgaagcg ggtactacgc
      421 atatgcaact gctgacagcg ctgaccacgc gcctggcgga tgatgagatt cgggcgcgta
      481 tacagtcggc gacgacgcct gatgagttgc tctcggcgct ggatgacaag ggaggcacgc
      541 aaccttctgc ctctttttcc aacgcgccaa ctatcgtctg cgtaacggcc tgtccggcgg
      601 gtattgctca cacctatatg gctgcggaat atctggaaaa agccggacgc aaactcggcg
      661 taaatgttta cgttgaaaaa caaggcgcta acggcattga agggcgttta acggcggatc
      721 aactcaatag tgcaaccgcc tgtatttttg cggctgaagt cgccatcaag gagagtgagc
      781 gttttaatgg cattcccgcg ctttcagtgc ctgttgccga gccgattcgc catgcagaag
      841 cgttgatcca acaagcgctt accctcaagc gtagcgatga gacgcgtacc gtacagcaag
      901 atacgcaacc ggtgaaaagt gtcaaaacgg agctgaaaca ggcactgttg agcggaatct
      961 cttttgccgt accgttgatt gtcgcggggg gcacggtgct ggcggtcgcg gtattactgt
     1021 cgcaaatctt cgggctacaa gatctgttta atgaagaaaa ctcctggctg tggatgtacc
     1081 gcaagctggg cggcgggctg ctcggaattt tgatggtacc ggtgctcgcg gcctataccg
     1141 cctattctct ggcagataaa ccggcgttag cgccaggctt tgcggctgga cttgccgcca
     1201 acatgatcgg ctccgggttt ctcggcgcgg tcgttggcgg attgatagcc ggttacttga
     1261 tgcgctgggt gaaaaatcac ttgcgtcttg gcagtaaatt caatggcttc ctgacttttt
     1321 atctctaccc ggtgctcggt acgttgggag cgggcagtct gatgctgttt gtggtgggag
     1381 aacctgtcgc atggatcaat aactcgctta ccgcctggct gaacggtctg tcaggaagta
     1441 acgcgctgtt gctgggtgcc attctcggtt ttatgtgttc ctttgacctt ggagggccag
     1501 tgaataaagc cgcttatgca ttctgcctgg gcgcaatggc gaacggcgtt tacggcccgt
     1561 atgccatttt cgcctccgtc aaaatggttt cggcatttac cgtaaccgct tccacgatgc
     1621 tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg gaaatccacc tggctgttag
     1681 ggctggcagg tattaccgaa ggggcgatcc cgatggcgat tgaagatccg ctgcgggtta
     1741 ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat tgtcggtgcg atgaatatcg
     1801 gcctttcgac acccggtgcc ggcattttct cgctcttttt acttcatgat aatggcgcgg
     1861 gcggtgttat ggcggcaatt ggctggtttg gtgcggcatt ggtgggggct gcaatctcga
     1921 ctgcaattct cctgatgtgg cggcgtcacg cggttaagca tggcaactat ctgactgatg
     1981 gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagcg gtatctcgcg ttcacatcac
     2041 cccgcatatg cactgggatc gggagtggta tttcaccacc gaagagtcac gcattctgct
     2101 ggtcaataat atggaagaga tcctgtgccg actggaacag gacaacgaat acaaatatta
     2161 cgtactcgac gggcaaacgg cgatcctcga agattatttc gcggtgaaac cggaaaacaa
     2221 agaccgtgtg aagaaacagg tagaagccgg caagttgatt atcggcccct ggtataccca
     2281 gaccgatacc acgattgttt ctgcggaatc catcgtccgt aatctgatgt acggaatgcg
     2341 tgactgcctc gcgtttggcg agccgatgaa aataggttat ttaccggatt cctttggcat
     2401 gtccgggcag cttccgcata tctacaatgg atttggcatt acccgcacca tgttctggcg
     2461 tggatgttct gagcgccacg gtactgataa aaccgagttt ttgtggcaaa gcagtgacgg
     2521 tagcgaagtg acggcgcaag tgttgccgct gggctacgcc atcggtaagt acttacctgc
     2581 cgacgaagac ggattacgca agcgcctcga cagttatttt gatgtgctgg aaaaagcgtc
     2641 agtaaccaaa gaaattttat tgccgaatgg tcatgaccag atgccattgc agcaaaatat
     2701 cttcgaagtg atggataagc tacgtgagat ctaccctcaa cgtaagtttg tgatgagccg
     2761 ctttgaagag gtatttgaga agatcgaagc gcagcgagat aatctggcaa ccctgaaagg
     2821 ggaatttatt gatggcaaat atatgcgcgt gcatcgcacc atcggttcta cgcgtatgga
     2881 tatcaaaatt gcccacgcgc gtattgaaaa taagattgtt aatctgctgg aaccgctggc
     2941 aacactggcc tggacgttgg gttttgaata ccaccacggc ttgctggaga aaatgtggaa
     3001 agagatctta aaaaatcatg cccacgacag tatcggttgt tgttgcagtg acaaagttca
     3061 tcgcgaaatc gtcgcccgct tcgaactggc tgaagacatg gcggataatc tgattcgttt
     3121 ctacatgcgc aaaattgccg acaacatgcc gcagagcgac gccgacaaac tcgtcctgtt
     3181 taacctgatg ccctggccgc gtgaagaagt gatcaacacc actgtgcgcc tgcgcgccag
     3241 ccagtttaat ttgcgggacg atcgcggcca gcctgtgccg tattttattc gccatgcccg
     3301 tgagatcgat ccaggcctaa tcgatcggca aattgttcat tatggcaatt atgatccctt
     3361 tatggagttc gacatccaga tcaaccagat tgtcccttct atgggctatc gcacgcttta
     3421 tattgaagcg aatcagcctg gcaacgtgat tgcggcaaaa agtgacgctg aagggattct
     3481 ggaaaatgct ttctggcaga ttgcgctcaa tgaggatggt tctctgcaac tggtagataa
     3541 agacagcggt gtgcgctatg accgggtatt gcaaattgaa gaaagctctg atgatggtga
     3601 tgaatatgac tattcacccg caaaagaaga gtgggtaatt accgcggcga acgcgaaacc
     3661 gcaatgcgat attattcatg aagcctggca gagcagggct gttatccgtt atgaaatggc
     3721 ggtgccgcgt aatctgcaag aacgcagcgc aagacaatcc actggcagag taggggtgga
     3781 gatggttgtc actctgagtc ataacagcag acgtattgat gtggatatca accttgataa
     3841 ccaggctgac gatcatcgcc ttcgcgtcct gatccctaca ccttttaaca ccgacagtgt
     3901 tctggcagat acgcagtttg gttcgctaac gcgccccgtg aacgacagtg caatgaacaa
     3961 ctggcagcaa gaaggctgga aagaagcgcc ggttccggta tggaatatgc tcaactatgt
     4021 tgccttacag gaagggcgta acggcatggc tgtctttagc gaagggctac gtgaatttga
     4081 agtcatcggt gaagagaaga aaacctttgc cattacgttg ctgcgtggcg tgggcttact
     4141 gggcaaagaa gatctgcttt taaggcctgg gcggccttcg ggaattaaaa tgccagtccc
     4201 ggactcacaa ctacgtggtc tgctttcttg tcgcctaagt ttattgagtt ataccggtac
     4261 gccaaccgcc gctggtgtag ctcagcaggc gcgagcatgg ctgactccag tacagtgtta
     4321 caacaaaatc ccatgggatg cgatgaagct caacaaagcc ggattcaacg tgccggaaag
     4381 ttatagtttg ttgaaaatgc ccccagtggg atgcctgata agcgcactta agaaagctga
     4441 agaccgacaa gaagtgattt tacggctgtt taatccggct gaatcagcaa cctgtgatgc
     4501 gactgttgct ttcagtcgcg aggtgatttc ttgctcagaa acgatgatgg atgaacacat
     4561 taccaccgag gaaaatcaag gttcaaatct atcggggcct tttttacccg gccagtcacg
     4621 gacgttcagt taccggcttg cctgaatagc aatcaaaccg aagccacata tgcgcggcca
     4681 gattgttgac aaagggcgct ttgttcatgc cggatacggc atgaacgctt tattcggtct
     4741 acaaaagcag gcaaattcaa tatattgcag agatgatgta gacactggca agcgtagcgc
     4801 atcaggtaat tttgcgttta tcttcactct caagccacgt atatgtggct ttatttttaa
     4861 caaaataat
//
LOCUS       DAZGOB010000225         4829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGOB010000225 DAZGOB010000000
VERSION     DAZGOB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.226"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            170..1144
                     /gene="hemB"
                     /locus_tag="ACHLIF_004906"
     CDS             170..1144
                     /gene="hemB"
                     /locus_tag="ACHLIF_004906"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO9608740.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1250..2101)
                     /gene="tauD"
                     /locus_tag="ACHLIF_004907"
     CDS             complement(1250..2101)
                     /gene="tauD"
                     /locus_tag="ACHLIF_004907"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO9608741.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTIAPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2098..2925)
                     /gene="tauC"
                     /locus_tag="ACHLIF_004908"
     CDS             complement(2098..2925)
                     /gene="tauC"
                     /locus_tag="ACHLIF_004908"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO9608742.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2922..3689)
                     /gene="tauB"
                     /locus_tag="ACHLIF_004909"
     CDS             complement(2922..3689)
                     /gene="tauB"
                     /locus_tag="ACHLIF_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO9608743.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3702..4664)
                     /gene="tauA"
                     /locus_tag="ACHLIF_004910"
     CDS             complement(3702..4664)
                     /gene="tauA"
                     /locus_tag="ACHLIF_004910"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9608744.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            4642..>4829
                     /locus_tag="ACHLIF_004911"
     CDS             4642..>4829
                     /locus_tag="ACHLIF_004911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608745.1"
                     /translation="MLRDEIAMIVCSPVLLCEQYSGIKTFIKRISRYRTDDFILSKKT
                     AKERLNMQLLRSNNWENY"
BASE COUNT         1110 a         1396 c         1240 g         1083 t
ORIGIN      
        1 agattaaaaa tattttcagt gaatatgaaa ttattgctat ttctattaca aagatgtcct
       61 ggtctctctt tcattctctt cttgtcaaca tcacgacaac tttcgtaaaa cactcctgcc
      121 ccgcttcagg tatactgtgc accttaattc cacaaacatc aggcagacca tgacagactt
      181 aatccaacgc cctcgtcgcc tgcgcaaatc tcctgcgctg cgcgctatgt ttgaagagac
      241 aacacttagc cttaacgacc tggtgttgcc gatctttgtt gaagaagaaa ttgacgacta
      301 caaagccgtt gaagccatgc caggcgtgat gcgcattcca gagaaacatc tggcacgcga
      361 aattgaacgc atcgccaacg ccggtatccg ttccgtaatg acttttggca tctctcacca
      421 taccgatgaa accggcagcg acgcctggcg ggaagatgga ctggtggcgc gtatgtcgcg
      481 catctgcaag cagactgtgc cagaaatgat cgtcatgtca gacacttgtt tctgtgaata
      541 cacttctcac ggtcactgcg gtgtgctgtg cgagcatggc gtcgacaacg acgcaactct
      601 ggaaaattta ggcaagcaag ccgtggttgc agctgctgca ggcgcagact tcatcgcccc
      661 ttccgccgcg atggatggcc aggtacaggc tattcgccag gcgctggacg ctgcaggctt
      721 taaagatacg gcgattatgt cgtattcgac caagttcgcc tcttcctttt atggtccgtt
      781 ccgtgaagct gccggaagcg cattaaaagg cgaccgcaaa agctatcaga tgaacccaat
      841 gaaccgtcgc gaggcgattc gtgaatcact gctggatgaa gcccagggcg cagactgcct
      901 gatggttaaa cctgctggag catacctcga catcgtgcgt gagctgcgtg aacgtactga
      961 attgccgatt ggcgcgtatc aggtgagcgg tgagtacgcg atgattaagt tcgccgcgct
     1021 ggcgggtgct atagatgaag agaaagtcgt gctcgaaagc ttaggttcga ttaagcgtgc
     1081 gggtgcggat ctgattttca gctactttgc gctggatttg gctgagaaga agattctgcg
     1141 ttaatttttt tcctctcaga aagggggagt taacctgttg aaagtacggt gaacgccccc
     1201 tcaccctaac cctctcccca agggggcgag ggaaccgtcc gcactcgtat caccccgccc
     1261 gataaaacgg tttatcccca aggatcgtcg cccgatgcat aatccgccgc tgtggcaggt
     1321 aatcggcatt ggcatagtgc tgagtcacgc ggttatccca aatcgcaata tcattcggtt
     1381 gccagcgcca acgcacctga aactccggtt tagtgatatg ggcgaataaa aagcctaaca
     1441 aggcttcgct ctctttctcg ctgacatcga caattcgcgt agtaaagcct tcattcacaa
     1501 acagcgcctg tttaccgctc accggatgcg ttcgcaccac cggatgtagc aacggaggat
     1561 ttttcgcgac cgcctcgcgc cagcgttgat gttcctcctc ggttttgcgg tatttgtatt
     1621 ccgggaacga tttacggaaa tcatgctccg cacgcagccc gctcagcagc tggcggaagg
     1681 gaacagagag cgcctcatag gccgcaatac cgctggtcca gagcgtatca ccgccggtcg
     1741 aaggtaactc tttagctgcc agaatcgccc ctgcgggtgg cgtttcaata aatgtcacat
     1801 cggtatgcca gttgtcgtta tccggcggat tatcgttatg ggtatccagc acgatgatct
     1861 cgtcaacccc ttcggcatga gggtaaacag ggtgaatatg cagttcgcca aaacgctggg
     1921 ccagcgcgcg ttgctgctgc ggcgcaatag tttgatcgcg cagaaacacc acctgatggc
     1981 gcagcaccgc atggtaaagc tgttcaaact gattatcgct taacgggcgc gtcaggtcgg
     2041 cacccgaaat ttgtgcgccg atatacggcc ccagcggggt aatgctcaga cgttcactca
     2101 ttgtacttct ccatgccagg gcgtcagacg gcgctgtaac gcgcgcaaac ccagttctaa
     2161 aagaaaggcg ataatcgcaa tcaccgcgat ccccgccagc accacgtcag tcgcgagaaa
     2221 ttcacccgct gactgaacca taaatcctaa accgcgcgtc gcggcaatca gctccgccgc
     2281 taccagcgta gaccagccca cacccagacc aatgcgtaat ccggtgagga tttccggcag
     2341 cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg ctggcaccca gcgactgggc
     2401 ggcacgaatg cgaacctgct gcgcgctttt cacccccgcc agcgccgaca tcgccaccgg
     2461 tgcaaaaatc gctaaataga tcagtaagat cttcgaggtt tcaccaatac caaaccagat
     2521 caccatcagc ggcaaataag ccagcggcgg caccggacga taaagttcga ttaccggatc
     2581 cagaatgccg cgcaccgtcg ggcttagccc catggcgatc ccgaccggaa taccgatcac
     2641 caccgccgcg agtagcgcca gcacaatgcg cgtcaggctg gctgccagat gctgccacag
     2701 cgtggcgtcc ataaatccct gcggtccggc aatggtgagc agttttgcca gtacttgttg
     2761 cggcggcggc aaaaacagcg ggctaatcag ttgcagcgcc gccaccgtcc accatacagt
     2821 gagtaaaacc gctaacgtgc caatgcttaa ggtcacctga cgcgagagcg gccagcgcca
     2881 tttcagccgc cgcgaatgca gtttttcatt aatgagcaca ctcatgagaa cgcctcccgt
     2941 tgctcaaata cgcggcttaa aacatattcg cgcatggcga tgaattgtgg atcggacttg
     3001 atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa agttgagcgg cagccgctcc
     3061 agcacgcggc cagggccggg tgaaagcaga accagttcag tcgccataaa caccgcttct
     3121 tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct cctgccagag tttcagcagc
     3181 agggtttgca tctggtcgcg ggtgaaggcg tccagcgcac caaacggctc gtcgagtaat
     3241 aacagctggg gattcgccgc cagcgcgcgg gcaatcccca cccgctgacg ttgaccaccg
     3301 gaaagctgcc agatatagcg tttttctgcg ccttccagcc ccactttttt cagcatctgg
     3361 tgcgcgattt ccagtcgctg cattttctct atacctgcca attgcaggcc gaacgccacg
     3421 ttgtcctgta cattgcgcca cggtagcagc ccttcattct gaaaaaccac cccgcgctct
     3481 gctcccggtc cctcaatacg cttacccgcc agttgaatgc tgccatgctg ataaggcaca
     3541 aaaccggcaa tcagattcag cagggtggtt ttaccgcagc cggacggccc cagcaccacc
     3601 agtagctcgc cgctttccag cgtcaggttg atatcttcca gtgccggttt gccgccataa
     3661 tcggcgtaaa gatgagagat ttgcagcatc tgcgcctcct tttattgcac gaagcgcgag
     3721 gtaacgtact ggctgtaatc attcgctaca gccgggacct tgccctgctc tttcaaaaac
     3781 tgcgcggtgt cgatgatcgc tttgttcacc ggtccggtca gttctgccgt ttgttgctgc
     3841 ggcgtcagat aggtattccc cttcaccagc cccggaacgt caccttccgg cacgccgctt
     3901 aaacgcgcca gtttgctgat gttttccggc tgtttcagcc acgcgtctgg gttagcaatg
     3961 tacggttgct gagcatcgat ggcgctttta gcgaacgctt tcacgacctc aggatgtttc
     4021 tcggcaaaat ctttgcgcac cacccagacg tccagcgttg gcgcacccca ctgcccgacc
     4081 tgttcagaat cggtcagcac cttgccgtct ttttccaggg cgttaaccgc cggtgcccag
     4141 acataagcac catcaatatc tccccgctgc caggcagcga taatcgcggg cggctgcagg
     4201 ttcacaatct ccacttgccc gggtttaatg ccccagtgtt tcagcgccgc cagcaggctg
     4261 tagtgggtgg tagagataaa cggtacggcg atgcgtttgc caatcagatc ttccggtttg
     4321 ctgatagttt tctttaccac cagcgcttcg gagttaccca gttttgacgc cagcaagaaa
     4381 acttcaatcg gcacctgttg gctggctgca accgctaacg ggctggaacc gaggttgccg
     4441 atttgcacat cgccagaagc cagcgcccgc acgatgctgg ctccgctgtc aaacttacgc
     4501 cagtccacgg ttgctccgct ttctttagca aaggtgttgt cggcctgagc cactttcgcc
     4561 ggttcggctg aggtttgata cgcaacggtg acgttcaccg cctgtgcctg aaaagcgatg
     4621 aatgccagtg cggcaagaag tgtgttacgc gatgaaattg ccatgattgt ctgctcccct
     4681 gtcttgttat gtgagcagta ttcaggaata aaaacattca ttaaaagaat tagtcgttat
     4741 cgcacagatg attttattct tagcaaaaaa acggcaaagg aaagattaaa tatgcaatta
     4801 ctccgttcta acaattggga aaattacta
//
LOCUS       DAZGOB010000226         4805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGOB010000226 DAZGOB010000000
VERSION     DAZGOB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.227"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..851
                     /locus_tag="ACHLIF_004912"
                     /pseudo
     CDS             1..851
                     /locus_tag="ACHLIF_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(891..1183)
                     /locus_tag="ACHLIF_004913"
                     /pseudo
     CDS             complement(891..1183)
                     /locus_tag="ACHLIF_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1263..2884)
                     /locus_tag="ACHLIF_004914"
                     /pseudo
     CDS             complement(1263..2884)
                     /locus_tag="ACHLIF_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3168..3578)
                     /locus_tag="ACHLIF_004915"
     CDS             complement(3168..3578)
                     /locus_tag="ACHLIF_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO9608746.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            3764..4732
                     /locus_tag="ACHLIF_004916"
     CDS             3764..4732
                     /locus_tag="ACHLIF_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO9608747.1"
                     /translation="MSQPGSLCVDIDVSKAILDIAASSAIEQFSVGNDSDGFDAIIAE
                     LRMYAIALVLMKATGGLETAIVCALQAEGFELAVVNPRQARDFARAMGYLAKTDSIDA
                     RVLSQMAEVLNRHPERERFIRALPDTECQVLTAMVVRRRQLITMLVAERNRLHPAHPL
                     NRKSINIIIKALEDELARLDADMNSHIHSHFKELAKRLSSIKGVGTMTAAALLAEVPE
                     QGNLSRREISVLVGMVPVNRDSGTMRGRRTIFGGRAGVRTALYMAALVATRFNPVIKV
                     FYMRLLAAGKPKKVALVTCIRKLLTILNAMLRKNEEWNESYHHVAP"
BASE COUNT         1183 a         1260 c         1164 g         1198 t
ORIGIN      
        1 cttattgctg cgctgataat atggaccgca aacctattga agatgtgatc ttcgaaatta
       61 ataactttat ctcgctcggt ggtcgcacca ttgttgatgc cacagggtct gagtctatcg
      121 gacgagatgc gcaggcatta cgagaagtcg cattaaaaac aggtctgaat attgttgctt
      181 cctccggtcc ctacctggaa aaatttgaaa gccagagaat tcataaaacg gttgatgaac
      241 tggcggcaac gattgataaa gaattgaatc aagggattgg cgatacggat attcgtgccg
      301 gaatgatcgg tgaaataggt gtctcaccga catttaccga agccgagcat aacagcttgc
      361 gggctgcctc gctggcacag attaacaatc ctcatgtggc gatgaatatt cacatgccgg
      421 gctggcttcg tcgcggtgat gaagtactcg acattgtgtt aggtgaaatg ggcgtctcgc
      481 caaataaagt ctctctcgca cactcggatc cgtcaggaaa agacgtggcg tatcagcgga
      541 aaatgcttga taagggtgtc tggctggaat tcgacatgat tggcctagac attaccttcc
      601 cgaaagaggg aatagcgcca ggggtgcagg agactgccga tgccgtcgct catctcattg
      661 agttgggata cgccgatcag cttgttctca gccacgatgt cttccttaaa caaatgtagg
      721 ctaaaaatgg cggtaatggc tggggatttg ttccagatgt ttttctggcc tatctggcgg
      781 agcgcggcgt cgataaaacg atcctcaaaa aactctgtat cgataatccc ggacgattat
      841 tgaccgcata aaaccattat cccccggcac actgcgccgg gggatgtcaa ttaatacccc
      901 gccgttaaat catccaccaa gcgcgagtcg gatgcgccgt acaactcacc atccgaccca
      961 accataatgc tttgcgtgct gcccatcgcc tcattcagcg ccactttctg accttttgct
     1021 tccagcagct tgagcgtatc cggactaaac ccttttcaac acgcagctcg tccggcaacc
     1081 actgatggtg gaaacgaggc gcattggtcg cttcggcaac attcattcca caattgatgc
     1141 tgttcaccac catttgcagc acggtagtga tgattcagct accggtaaca aagtgagtac
     1201 ataaaggcca tactgtcacg aacagtgcaa aagtggctct tccgcaacct aggtgaagat
     1261 tttcatgcca gtggactcat taccctccgc ctaagtagtt caaatccggc tcgaccatac
     1321 atctggcgta tcagcacctt caggcgattt acatgacctt cgacaacacc attactccag
     1381 cgactgctga ttgcctcaca tatagccgca gcatcagctt ccatccccgc agctacgcac
     1441 cgaagttctg ctgagccgct ttcgtggact cgagtgaacc agctgcttag ctgtgattta
     1501 ttttaggttt tcagaatacg gtagaactcg agtaccagtt gctgtgctat tttcaactcc
     1561 ggttcttttt cacacatcag actaataaat cgggatgcat aattttcttc gcccctgatt
     1621 attctccagg gcatcaaccc gcggcttacc cggaacactg aaggaagtct tgcggcagta
     1681 gtaaccggtg ggttccagcc tttacgccat ttggcaacag tatccctgac gatggtttca
     1741 ctgcctgtcc ccccccctgg gccaccattt cccgccatat ccggctggca ttataattac
     1801 cactttctcg ttgctctttt aaccactccc tccatgggtc cagaagccct cgctttggag
     1861 gccgggttga catttcaggg aatgttcctg aacgcaccca gcgactgact gtcactcgcg
     1921 acaggcctgt aatacgggat atttccctga aactacatcc cttgttatgc agggccataa
     1981 cctctgtcca atgctgatgc cgttttttgc gggtttgctg tttaaggcgt tccggacgac
     2041 ggagcgatgc tacaggcaca gatatttctg gctcaggtga tttcttcagt gataactctc
     2101 tgacaacaag acgtatcaga ggcatatgtc tgtacatcat tcgttcaggc tcatcaccaa
     2161 tattttttag caggtgccat cgatcggcca cctgtctggc ctgaggtgca ccttcacgtg
     2221 ctgctgtcgc atagactcca ctgcgatcac gcgagacaac ctgtatttcc ggatattttc
     2281 tgaaccaggt cgccagcgta cgctgatcac ggccgggaag caggacgagg ggacggtgag
     2341 tatcaagatt gactattaac ataccgcagc agtggccccg atgccacgcc cactcatcaa
     2401 taccgacatg aggcgcgcct gactgtttcg tctccggggt attgataacc ctgcgaagaa
     2461 gagtatcagc actgcaacgg agacccgctg ccgttgcagc ccgttttcca gcctcacctc
     2521 cagctattaa tcccagttga cgttgtaaat tttgtaacgc ctgtgaagac tgttggtgtg
     2581 aaccggcgaa gggagcaagc gactcggcaa aaattttacg tgaacaagct gggttacggc
     2641 agtaccagtg gcggacggaa aatacaagcc atagcgtctg tcctgatcag ggtagatgct
     2701 gtatccggcg ccgacaacaa ctatgaacag agtcgctacg cttaaagcat tcaggacatt
     2761 gtgctgtttt gcgttttccg tggagatgga gggttatacc gtcagagcta ataatttgtc
     2821 gactgcatcg ccatccgcaa ggtaactgta gaagtgtctt aagagagtgc attgggttgc
     2881 ccataactta aaacagctta gatctttgtt cgccagaaag tttcatccat tttcttccac
     2941 ccaggttgcg gaagaaccac ttttcatccg tcgttgacag atgtgaagta tgcgggagaa
     3001 aaagtgaata tctaactgta ataactcaga ccggtacgag tactcggggc gtctacaatc
     3061 attcagggca aagtgctaaa atttctctat cccgggatgc ccctaccaac gcagcactcc
     3121 cggagatatc attacagctc tgcaactgat ttccgcgact gaaccgctca gttcctatcc
     3181 ttccagttgc cttcctgcag ccccccctcc ctgctgtata ccccagtacc acaacgcggg
     3241 cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
     3301 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     3361 gggtcaagcc aggtgcatcg cagcaggatt ttcgcagcaa cagtcatctg gtcatgttca
     3421 aaccacggcg gttgtccgtc atttccccgc ttaagtgtta acggacaacc acaggacaca
     3481 cagcacaaaa cgtcctgtcg gttgtgcttc gcggcttctt tcgccgtaac aaaatggcct
     3541 ttgctgtcat tcgccatata gcagttcaga attcgcatat ctcctccttg tatcttgaca
     3601 attggttaaa ataactgtga ttagatttgg caaggtgtat gcagcccgat ccctcaaggg
     3661 gttcatagcc cgtagctcag cgaatggcgc atacactgag ccactcatct gaaacccgaa
     3721 cagttgtcag tacgccataa cgcatttgca aggatgggta atcatgagtc agccaggttc
     3781 actttgtgtg gatatcgatg tttcgaaagc gatactggat attgccgcca gcagcgctat
     3841 tgaacaattt tctgtcggca atgacagtga tggctttgac gccatcattg ctgagttgag
     3901 aatgtacgcc attgcgctgg ttttaatgaa agccactgga ggacttgaaa ccgccatagt
     3961 ctgtgcgctt caggcagaag gttttgagct tgccgtggtt aaccccagac aagctcgtga
     4021 ctttgcccgc gccatgggat acctcgcgaa aactgatagt attgatgcca gagtgctttc
     4081 ccagatggct gaggttctca accggcatcc ggagcgggaa cgctttatca gagcactgcc
     4141 ggatactgaa tgtcaggttc tcactgcaat ggtagttcgt aggcggcagt tgattacgat
     4201 gctggttgcc gagagaaacc ggttgcaccc tgctcatcca ttgaacagga aaagcattaa
     4261 tatcatcatc aaagcactgg aagacgagct tgcgcgacta gatgctgaca tgaacagcca
     4321 tatccatagc cacttcaaag agcttgctaa gcgactgagc agtattaaag gtgttggcac
     4381 catgacggct gctgcgctgc tggcggaagt acccgaacag ggtaacctct ccaggcgtga
     4441 aatcagtgtg cttgtcggta tggttccagt aaaccgggac tcgggcacga tgagagggcg
     4501 acgaaccatc tttggcggca gggctggtgt gcgaacagcc ctgtacatgg cagcgcttgt
     4561 cgcaacccgc tttaacccgg taataaaggt gttttacatg cgactgcttg ccgcaggaaa
     4621 acccaaaaaa gtggcgctgg ttacctgtat acgcaagctg ctgactatcc tgaacgcaat
     4681 gctcagaaag aacgaagaat ggaatgaatc gtaccatcac gttgctccat aatttttacg
     4741 ttcaagacag ttgctgagct gtccggggaa gactgaaacc tccggtctgg cagtgtgtgc
     4801 agtac
//
LOCUS       DAZGOB010000227         4772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGOB010000227 DAZGOB010000000
VERSION     DAZGOB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.228"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(57..632)
                     /locus_tag="ACHLIF_004917"
     CDS             complement(57..632)
                     /locus_tag="ACHLIF_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO9608748.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(701..1279)
                     /gene="terD"
                     /locus_tag="ACHLIF_004918"
     CDS             complement(701..1279)
                     /gene="terD"
                     /locus_tag="ACHLIF_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO9608749.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1328..2368)
                     /gene="terC"
                     /locus_tag="ACHLIF_004919"
     CDS             complement(1328..2368)
                     /gene="terC"
                     /locus_tag="ACHLIF_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO9608750.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2391..2846)
                     /locus_tag="ACHLIF_004920"
     CDS             complement(2391..2846)
                     /locus_tag="ACHLIF_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO9608751.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(2869..4026)
                     /locus_tag="ACHLIF_004921"
     CDS             complement(2869..4026)
                     /locus_tag="ACHLIF_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO9608752.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4026..4607)
                     /locus_tag="ACHLIF_004922"
     CDS             complement(4026..4607)
                     /locus_tag="ACHLIF_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO9608753.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         1203 a         1281 c         1156 g         1132 t
ORIGIN      
        1 agcggatcga tacccaaaaa aaaccccggc attgccgggg tcagactcag cgtcttttag
       61 acattgacgc cgtgctggga agcaagcgct gacagaccac cggcaaaacc ctggccaaca
      121 gctttaaact tccactcagt accatggcga tacagttcac cgaagaccat tgcggtttcg
      181 gttgaggcgt cttcagacag atcgaaacgg gcaatttccg tcccgttgtc gttgttgtaa
      241 acgcgcatga agctgttgct caccatgccg aagttttgtt tacgcgcttc tgcatcatag
      301 atggtaacgg caaataccag ttttttgatg tctgctgaga ctttggtcag atcgattttg
      361 acctgctcat cgtcgccgtc gccttcaccg gtacggttgt cgccctggtg ctctactgcg
      421 ccatcagggc tggttttatt attgaagaaa atgaaatggg catctgacag tactttaccg
      481 tcttcaccta ctgcgaatac ggaagcgtcc agatcaaaac cctgaccatc ggttacacgg
      541 gcatcccagc ccaggccaac catagcgaca ttcatggttg gtgcttcttt ggtcagagat
      601 acgttgccgc cttttacgag agaaactgcc atttttagct cctgcaaaca gttgaatgag
      661 gctgaattaa cagccccaga tatgaaaagt tacttttcga tcaggacgcg ttaatgccgt
      721 actgagcaca tacagatgcc agaccaccag cataaccctg gcctactgcg cggaatttcc
      781 actcaccatt gtggcgatac agctcgccga acagcatggc agtctcagtg gacgcatctt
      841 cggtcagatc gtagcgagcg acttcagtct ggttatcgtc attaaccaga cgaataaacg
      901 caccggatac ctgaccaaag ctctggcgac gagcctgagc atcgtggatg gtcacaacga
      961 agatgatctt gtcaacttca gacgggacgg cgtccagttt aattttcagc gattcatcat
     1021 caccatcgcc ctcaccggtg cggttatcgc cggtgtgcgt tacggaaccg tcggatgacg
     1081 tcaggttgtt atagaagatg aaatctgaat cgccgcgcac tttgccgttt gaggccagca
     1141 ggaatgctga agcatccagg tcaaagtcct gaccgtctgt tgaacgcgca tcccagccaa
     1201 ggcccaccag gacatttttc attgacggag ctgctttact cagggagacg ttcccgcctt
     1261 tggaaagaga aacactcata aaataacctc ttcgattagt aattgttcag gttaacactt
     1321 aaggggatta actccccttt tcctcagatt caggtttgcc cgggaacatg acgcttgcga
     1381 gaatgcccag cgccagtaca cccagtacaa catacaggct ggttgttgcc gcgatgctgt
     1441 aaccatgatg ccagatgtga tcggtcgcat tcaggccgag ttttgccgcg atgaagaaca
     1501 gcagcacgat aacggccttc tccagatgaa ccaggtactg tttcagtgcc tcaaggacaa
     1561 aatacagagt acgcaggccc aggatagcaa acatcatggc actatagacg ataagcggtt
     1621 cacgactgac ggcaatgatt gccggtaccg agtcaaacgc gaacattacg tccgagagtt
     1681 caaccacagc cacacacagg aacagcgggg tcgcatacag cgccgctttt ttaccacggc
     1741 caatggtgac atcgctgttt tctggtttcg ccagttcagc atccacttcc ttctggttaa
     1801 gtaggaaggc atgccctctg agcttcggcc agatagggaa gaagcgttta accatacggt
     1861 aagccagatg ctgggagtaa tcctcaattt catcatcgtc atcaccgctt ttaagcatca
     1921 tgaccgccgt ccaggcaacg ataatagcga agacaacttc aacgtacggc cccagactca
     1981 gcaggctcgt accgatggcg acaaagatgc ccctgaagac gatggcacca atgatccccc
     2041 agtagagaac acggtggcga taacgatccg gaacggcgaa ccaggagaag attgccatca
     2101 tgacgaacag gttatcgacc gacagcactt tctccagcgc ataacccgtg acaaacagac
     2161 tggcaacctc agcaccgtgg tggatataga ggaaaccggc aaatgccatc gcaaccacaa
     2221 cccagaatac ggaccagagc gcagcactct tcagcgagat tggcttgtca tcacggtgca
     2281 taaacaggtc aataaagatg gcaccgactg aaagcgcaat aaaaacaatg accgtttcag
     2341 tcgggaagcc gatgtgtgtg gaaaccatat tttaccctta gggatgtgga tcagaggcca
     2401 aattcagccg ggtcgaagcc caacgcgata gcgatctcgc gggaggccag tttctcgtcc
     2461 tgatcgaagt taccgtcact tttcgcaacg gcaataccaa cacgtaaggc cagctgagcg
     2521 gcctcaggct gatctttcag cgccaggatg tatttcatgg tttcgccctt gccaatttca
     2581 acatcgaaat cgaagcttga aaccagttta ttgaagaact caatcacctc attggtatcg
     2641 aagaccttca gctcttctga agagcgcaga aagcccatca ttttctgctt ttcttccgaa
     2701 cttacgccgt cactcgatac ggcaatacgg gcacatacag caacggtgcc ctgcatgaat
     2761 tttttgtttt tgaaacggcc aacctggcgg gtcagttcgt cacggcctga gttaatggca
     2821 cctttaactt tgtcgaaaaa gctcatacct gtttcctttg ttgctgtgtt atttagaacc
     2881 ggcgctccag cgaaatcccc agccaaatgc ccggtccatt tcgtcctggc ctttgaagta
     2941 ctggttaatt cgctcgactt taattgcgcc gttttcgttt acgagtctgg caatggcgca
     3001 caatgtgcga cggttctcac cttcggtcag gcgggtctca atgggcggtt gatccggcac
     3061 atgaatggtg actacaccat cagtcttgtc ccagctggga acaccttcat aaataaaggc
     3121 gtaaatcagc acttcgcgga tatgcttcca ttcacgtcca ttgatatgca gccactcgcc
     3181 gtctgataca tcacccgtcc ggtcatcgcc cttgagctga acatacggtt catcgcgata
     3241 atcaccgaat gcattaccga gcgcctgaat gaccgattta tacccgtcct gaagctcaac
     3301 aaaggccccc agatccaggt cgattccttt ggagccgaac atacctgcaa agcctgattt
     3361 gctactgccc cggtgccagt tcaggttgat ccggatttca ccgaagttat cccgtttggt
     3421 caggctgatt gcaggcttct cttttgtcag cgataccttg ctcaggctga tttttgtctc
     3481 aactggcgga ggggttacaa ccggaggagt tggggctgcg ggagccggtt cgtcggcaat
     3541 atttacgccg aaatgctctg ccagcggttt aagtccaccg ttgaacccct gcgcaacgaa
     3601 gcggaatttc cagtcattat tacgacggta aaattccccc agaattaagg ctgcttcctg
     3661 acggccgctc agttcgacac tgccgctaac caggccggta gccccttgct ccacgtcgat
     3721 ggaaagatta cgaagaccgg acaccgtctg cccaccgtca caggtcacgg tgaacgcaat
     3781 tttctggaca tcaggtttca gccggttgag cgcgacagta aaggttgagt actggccttc
     3841 actgaccagg ctgacggtac catcatcgtt acgtggctga ccataaaaca ccatgtccgc
     3901 atcaccctgc accttcccgt cggcatacag gcgaaacgcc gaagcatcca cctggccgcc
     3961 cgaggtgatc cggacccgca actcctgggc tggaacgggg gcattgcccc ccggcgtcag
     4021 gttcattagc gaataaccgc tgaaacgata tcggagtgca tatcgtcaat tgtgcggccg
     4081 cgacaggcat ggccaagggc ggtaaagtcc cagttgccgt tattacggcg cagcgaagaa
     4141 ataacgatcc cggtatgtga gccctgctca gtcagcttgt agcgagccag ctctttgccg
     4201 gtttgatcga caacgcggca gaaagcgttt tcgacgtcat tgaacgactg accacggaag
     4261 ctgtttaccg taaacgccag gtattcaacg tttgcaggca ggcgggagag gttgacctta
     4321 atcacttcat cgtcaccgtc accctcaccg gtcaggttat cgccactgtg cacaacagcg
     4381 ccgcaggtgg atttcagttt gcggaaccag atggtgtcaa ttgttttacc tgtgctgtcc
     4441 attaatacac agccagcatc gagatcgatt gagtcgttgc ctccaaacaa tcctccaaga
     4501 agaccttttt tcttaaccgg atcccaaccg agaccgaaat gaagctggct taatgcagat
     4561 gattctttgc tgagtgatac cgtctggttc ttaactaatg aaaccatgtt ttattcctta
     4621 aaatggtaat gaccgagaca atcaaaggta tatttagtaa ctgacgaaga aaatcatatc
     4681 atgatgaagt tctctgacaa gggaaatttt tgtggttttc tgtaaaaaca caaaaagaaa
     4741 tccttttcag ggtgttagtt tttctatttt ta
//
LOCUS       DAZGOB010000228         4730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGOB010000228 DAZGOB010000000
VERSION     DAZGOB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.229"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(465..680)
                     /locus_tag="ACHLIF_004923"
     CDS             complement(465..680)
                     /locus_tag="ACHLIF_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608754.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPIDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            complement(767..2143)
                     /locus_tag="ACHLIF_004924"
     CDS             complement(767..2143)
                     /locus_tag="ACHLIF_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO9608755.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDTSSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERNKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGPLGTVYQRFCNGHFVACDQDEARQICTASNAPASRGR
                     RYAQGADV"
     gene            complement(2140..2961)
                     /locus_tag="ACHLIF_004925"
     CDS             complement(2140..2961)
                     /locus_tag="ACHLIF_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9608756.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEIIDSLLMADLT
                     VRQMKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAESANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            complement(2948..3109)
                     /locus_tag="ACHLIF_004926"
     CDS             complement(2948..3109)
                     /locus_tag="ACHLIF_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608757.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(3145..3423)
                     /locus_tag="ACHLIF_004927"
     CDS             complement(3145..3423)
                     /locus_tag="ACHLIF_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO9608758.1"
                     /translation="MELTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVEDEELAELAKKVAMVLTKEKPQDCGNSFEA"
     gene            complement(3533..3757)
                     /locus_tag="ACHLIF_004928"
     CDS             complement(3533..3757)
                     /locus_tag="ACHLIF_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9608759.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGLVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            3869..4576
                     /locus_tag="ACHLIF_004929"
     CDS             3869..4576
                     /locus_tag="ACHLIF_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO9608760.1"
                     /translation="MKTFAERLNAAMSSAGVSQSQLADMVGISQPAIQKMSSGKTNGS
                     RKMVELANALKVRPEWLSSGIGEMRDGAHEEPSNVRDSSLKAVVWEDIKRNDDEFVAL
                     PLLNVSLSAGSGSCELEESSEFSLVFRKHYLKKMGVSERSAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTTIRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEF
                     HETVRIIGRVFWSSHSW"
BASE COUNT         1179 a         1182 c         1042 g         1327 t
ORIGIN      
        1 tcactctcca tcgatgattt tttgggttac caataatatt tgatagtcgc cataattatc
       61 ggtagcaacg cgcagacaac tgaaaaccgt aaaacaaaac ctactcccaa tatgcgatat
      121 ccattattcc agagaacaaa actcatcatc agaaggaatc catgaaaaat cgcgacaaga
      181 aataaactac aaataaatgc atttaccatc ggtacttacc cctcgctctt aatccaataa
      241 aaaagggttt gcttcactga acactccttt attttttatg cctgtaaccc cattcttcca
      301 gcaaccttgc ggcgtaccac ccaagaaaca aaggaaagaa cattacaatg agatattccc
      361 cgccacggtc aatgttcgaa attgaccaga ttacgatgta accagtgcag gacaggaata
      421 ttacaaaccc caaaaagcta cttcttcgac tcatgctcac tccttcactt taaatccaga
      481 ctccggatta ttctgttgcg ctgaaactca ttgttgagtt tgaacaaccg tcgaaggaca
      541 cggtcacgcg gatagcgtcg tgcagcaggt gaatgctcat acaactcatc aatcggcaaa
      601 ctggacgatg aacgataccg ataccaacgc accaactctt cacgaaaatt agccctgaca
      661 agctcagcta tcgtactcat ttcttaaagc ctccaattcc ctctccccca aataaaaagg
      721 cctgcgatta ccagcaggcc tgttattagc tcagtgatgt agatggtcat acgtcagccc
      781 cttgtgcata tcgtctgcca cgcgaagcag gtgcatttga tgctgtgcaa atctgtctgg
      841 cttcatcctg gtcacatgca acaaagtgtc cgttacagaa ccgctggtaa accgtaccaa
      901 gtgggccaaa ccggtttttc gtcacaatga tttcagcaaa tggtgcggcg ctactgttct
      961 cgtcatatac cgcttcccga tagagcatga tgattgagtc tgcgtcctgc tcaatgcttc
     1021 ctgaatcacg caaatctgcg tttgtcgggc gtttgtttgg tcgcttctca acatcgcgcg
     1081 aaagctgact tagggagata acaggcgttt tcaggtcttt cgccatcgcc ttcaggcttc
     1141 ctgagatgtg agcaattgcg aggtcgttgc ggtctgcttt cggcttctca atcaggccaa
     1201 gataatccgc catgatgagt gacaggtttg gattttcctg tttgttccgc tctgcgattg
     1261 agcgaatttc ttctaccgat aaccgcgagg catcgactac ccatacatcc aaatctgcaa
     1321 gctgactcat gccgttagca acacgcgccc agccttcgtc atccatcgat gcaggatttc
     1381 gcagtacgct aaccgacatc ctcccggcgt tggcaatgct ccgctctgca atctgcaatg
     1441 cgctcatttc catcgagaaa atcaacactc cgcgccggac gtcagaacca ggaataacgc
     1501 ggcttgcaac accttcggca atcttcagcg ccagttcggt tttccccata ccaggacgag
     1561 cggcgataat caccaggtct tccgcgttca tccctccggt gatggcgtca agttcttcga
     1621 ttccggtctt cagggtatct gactcttctc cgttcctcag acgcctgtca agcgtgtcag
     1681 tgtagtcagt gatgatttcc cctaaccgta caggtttaac ctcgtcacgg ggctttctga
     1741 tggctgagag acgctttaca agctcgtcca tcgcctgact cgatgtatcg atggttccgc
     1801 tctgaattgg ttcacgcatt tcgtccatga tttccagcac cagacggcgg tgatagttat
     1861 ccgcgaccat tccggcatat cccttcaggt ttgcggcact cgggcagttt ttgctggtca
     1921 tcaggattga cgtgaaatgc tcctctccgc acgcctcggc aaccatcagc gcgtcgatta
     1981 ggtttctgtt tctcgcctgc ttacggataa cttcgaaggc tttccggtag agcggaattg
     2041 aaaacgcttc cggctccagc gttgccagaa cgtcgctggc ggttggtgtt aatccaccaa
     2101 tcagcaagcc accgataacg ctcgcttcga tatcctgtct catgcaatcc ccctgtctgc
     2161 aaacttccct tcccgaactc ccgttaacga gtcttccctc agcaggtaat caaaatctgc
     2221 cgtccagccc gtgtcgttgt ctccgaagta aaacggcttg gcctgatgca caaacgccct
     2281 gacatacgct ctgaaaccgt ccacgtttgg cgttttcagt tgcgggatga ttttcttcag
     2341 gcggcgtttt cgtttctcgt tgaccgcaac agcgtgtgga agtctgtcac cgacttcggt
     2401 gttgtaggcg ttcaggaagg attcgtagtc gattcgttct gccttgcgac gttcaggttt
     2461 aacctgccca tcgccgcccc cgttaggggg taagggggta tttgtattta ttgtcttttg
     2521 tatattgtct tttgtgttta gctgacttgg cttataccca ttagccgact cggctaatgt
     2581 tttattagct gttttagcta atgttaagct gtcctggcta atccactgag aaaccacctt
     2641 gttcactccg attttcacgc catcagcaat gaggaattta cgctcaataa gctggcgctt
     2701 ggcagcgcaa acatgagtgt gatgaatacc tgtcatggct gctatctgcg tgtttgtgag
     2761 tcgatccatc ggcttattga atccgtatgt cttgcgcatg atagcgagca tcaccttcat
     2821 ctgccggacg gttaaatcag ccatcagcag actgtcgata atctcgttag caacgcgcat
     2881 gaaaccatct tcggtatctg ccacgcgatg ctccacgacc tccagttgag gcctgtaatc
     2941 agctaactta acgacgccca tgtttcactc ctgctttggc tagtctgtaa acaccaacaa
     3001 ggcgctctgc gaacgccctg ttatttgctg cggctaccac taatccctca ggtgaatcag
     3061 ggtgtcgaat ctcttctttt tcctggtatt tcttacgacg ttttgtcata attactcctg
     3121 tggattgata cagtctttct acaatcaggc ctcaaaactg ttgccgcagt cttgaggctt
     3181 ttcttttgtc agcaccatgg ctactttctt tgccagctcc gctaattcct cgtcttcaac
     3241 accccactcc agcacagcca gaagcatggc catctttggg ataaagctgt ctttccatcg
     3301 cgaaatttgc gattcattga tccctaatgc atcagcaacc tttcgctgac cacgtacagc
     3361 aattcgattc aggatgttgc ttgtaattgc attcgctttc ttgcgagtac ttgtaagttc
     3421 catatgtaag tatttcctta acaaataaga agttatgcgc atcaacttat gcgcgttgta
     3481 ttcccgcatt tcggcgggaa tgaggaccat gactgttaaa gagcggtgtt acttatgctg
     3541 cctgattcgg ttttggaaac aggtgtggca aatcggggcg aatttcgtaa gccttgatct
     3601 gccctccagt ggcgttaacg atggcggtaa ctttctctgg agagaccaac ccgcctttca
     3661 gccatttgtg tactgctggc tgcgttacac cacacttgtc ggcaaggcgc ttttggctac
     3721 cgacaatttt caaggctcgt tgaattacta aattcatgag catacctctt gtggtcatta
     3781 cttataacca aagataactc aagttataaa aaacagcaat aacctttgtt attttacttt
     3841 ggataaccgt agttatagat ttgtgggtat gaaaacattc gcagaaagac taaatgcagc
     3901 catgagctca gcaggggtat cacaatcaca gcttgctgac atggttggaa tatctcagcc
     3961 agccatacag aagatgtcgt ccggtaaaac aaacggatct cgcaagatgg ttgaattagc
     4021 caatgcttta aaagtgcgcc ctgaatggct tagttctggt attggtgaaa tgagggatgg
     4081 tgcacatgaa gaaccatcca atgtccgtga ttcatcttta aaagctgtgg tatgggaaga
     4141 cattaaaaga aacgatgacg agtttgttgc gttgcctctt cttaacgttt cgctttcagc
     4201 tggaagcggt agctgcgagc tagaggaatc atcggagttc tctttggttt tcagaaagca
     4261 ctatctgaaa aagatgggag tatctgaaag atcagccaag ctagttaggg ttgtagggca
     4321 aagcatggaa ccaacgcttc acgatggcga tgttgttggt gttaacacgc aagataccac
     4381 aatcagagat ggtaaaacct acgctatttg ccagtctgat ttgttacgag taaaaacatt
     4441 aatcgccacc cctacatcgg tgataatcag atcaataaat cgcgaagagt acccggatga
     4501 agtaatggat agagatgaat ttcatgaaac cgtaaggatt attggcagag tattctggtc
     4561 gtctcatagt tggtaaccga taatcagaag aagacttacg gaagtgcgga gggataatgg
     4621 aatttctgat agtttttgtt gttgttttgg tcatcattct ttttgttttg ctaagcatta
     4681 gtaaaaagct atctcaaatg attgaacata gctctaatcg tgcaaaagaa
//
LOCUS       DAZGOB010000229         4592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGOB010000229 DAZGOB010000000
VERSION     DAZGOB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.230"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..596)
                     /locus_tag="ACHLIF_004930"
     CDS             complement(<1..596)
                     /locus_tag="ACHLIF_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO9608761.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(711..786)
                     /locus_tag="ACHLIF_004931"
     tRNA            complement(711..786)
                     /locus_tag="ACHLIF_004931"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Thr,seq:ggt)
     gene            complement(793..867)
                     /locus_tag="ACHLIF_004932"
     tRNA            complement(793..867)
                     /locus_tag="ACHLIF_004932"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Gly,seq:tcc)
     gene            complement(984..1068)
                     /locus_tag="ACHLIF_004933"
     tRNA            complement(984..1068)
                     /locus_tag="ACHLIF_004933"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Tyr,seq:gta)
     gene            complement(1077..1152)
                     /locus_tag="ACHLIF_004934"
     tRNA            complement(1077..1152)
                     /locus_tag="ACHLIF_004934"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1117..1119),aa:Thr,seq:tgt)
     gene            1514..2464
                     /gene="coaA"
                     /locus_tag="ACHLIF_004935"
     CDS             1514..2464
                     /gene="coaA"
                     /locus_tag="ACHLIF_004935"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO9608762.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHLIF_004936"
     CDS             complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHLIF_004936"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO9608763.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHLIF_004937"
     CDS             complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHLIF_004937"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO9608764.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1173 a         1162 c         1040 g         1217 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcatactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaagat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      481 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      541 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      601 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      661 acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat
      721 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      781 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      841 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      901 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      961 ctacgccatc gcgtggccga aattggtggt gggggaagga ttcgaacctt cgaagtctgt
     1021 gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccgg acttgatggt
     1081 gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct ctacctactg
     1141 agctaagtcg gcatcaagta gcgcgcattc tatggagaca tgcgagttca tgcaactaaa
     1201 aaattgcata atttgtttta ttggtcacat tttatgcgac acgatgaaga aacagccgaa
     1261 cggagggcag attagcacac tttttcaaca tcattgtgct caacaatgcg ctcctgctaa
     1321 accataattc tttttatcag atggaatatc tgtcacattg cttttcaacg atagcttcct
     1381 ggcagagatt ttttcttatt attcctcccc atctggtgtt accctcctgc ccattaaccc
     1441 attcaacaga actgtgacgc gccatggcaa atatcgcttt gccgatagag ctatgaccgc
     1501 cagaaacatg cttatgagta taaaagagca aacgttaatg acgccttacc tacagtttga
     1561 ccgcaaccag tgggcagctc tgcgtgattc cgtacctatg acgttatcgg aagatgagat
     1621 cgcccgtctc aaaggtatta atgaagatct ctcgttagaa gaagttgccg agatctattt
     1681 acctttgtca cgtttgctga acttctatat aagctcgaat ctgcgccgtc aggcagttct
     1741 ggaacagttt cttggtacta acgggcaacg cattccttac attatcagta ttgctggcag
     1801 tgtcgcggtg gggaaaagca caaccgcccg tgtattgcag gcgctattaa gccgttggcc
     1861 ggaacatcgt cgtgttgaac tgatcactac agatggcttc cttcacccta atcaggttct
     1921 gaaagaacgt ggtctgatga agaagaaagg cttcccggaa tcgtatgata tgcatcgcct
     1981 ggtgaagttt gtttccgatc tcaaatccgg cgtgccaaac gttacagccc ctgtttactc
     2041 gcatcttatt tatgatgtga tcccggatgg agataaaacg gttgttcagc ctgatatttt
     2101 aattcttgaa gggttaaatg tcttacagag cgggatggat tatccacacg atccacatca
     2161 tgtatttgtt tctgattttg tcgatttttc gatatatgtt gatgcaccgg aagacttact
     2221 tcagacgtgg tatatcaacc gttttctgaa attccgcgaa ggggctttta ccgacccgga
     2281 ttcctatttt cataactacg cgaaattaac taaagaagaa gcgattaaga ctgccatgac
     2341 attgtggaaa gagatcaact ggctgaactt aaagcaaaat attctaccta ctcgtgagcg
     2401 cgccagttta atcctgacga aaagtgctaa tcatgcggta gaagaggtca gactacgcaa
     2461 ataatttgca ggggagcgaa tactcccctt tcttattttt ctgcactacg cagggatatt
     2521 tcaccgccca tccagggttt tattattcca tcctgctcaa gtaataaagc cccctgttta
     2581 tctattccgc gtgaaatgcc aaatatttct ttatcaccaa tgataagttt cactgggcga
     2641 ttaataaaat tatccagctt ttcccagcgc gacagataag gtgccaatcc ttcttgttcg
     2701 aagagttcca acgcagcacg taattcacgt attagcatgg ccgccaacgt attacgatcg
     2761 agattgatcc ccgcttcctg cagcgtgatc cacccctgat taacgacact ctcttcaaca
     2821 cggcgcattg ccatgttgat cccggctcca atgactattt gcgccgcatc gccagttttg
     2881 ccagtcagct ccacaagaat gcctgccagc ttgcgatcct gcagatagag gtcattaggc
     2941 catttgacac gaactttatc tgctcccagc ttgcgtaata cttccgccat cacgataccg
     3001 ataaccagac ttaaaccaat cgccgccgcc gggccttgtt ccagacgcca gaacatcgac
     3061 aaatataagt ttgcgccaaa aggcgaaaac catttccgcc cccggcgacc acggccagcc
     3121 tgctggtatt ctgcaacaca ggcatcgccc gatttaagct ctccgatacg atcaagaagg
     3181 tactgattcg tggagtcaat aactggcagc acggctacac taccgccatc cagctgaccc
     3241 aatatctgtt cagcattaag taactggatg ggctcaggca ggctgtatcc tttacccgga
     3301 acggtaaaga catcaacgcc ccagtcacgc agtgtctgaa tgtgtttatt aatagccgcc
     3361 cggctcattc ccagcgtttc acccaactgc tcgccagagt gaaattcacc gttcgctaac
     3421 agggcaatca atttcagtgg cacggtgtta tccttcatga aattgtctcc actgcgctca
     3481 cttcacctga tgcaccaata aagcggactt caggctcaag ccagacatta aatttttcac
     3541 ccactttttg tcttacatgg tgtgccagtt gcaccacatc ttcgcttttt gcattgtctt
     3601 cattaatgag aactaacgcc tgttgacggt gcaccgcagc cccacccatt tgcatccctt
     3661 ttagctggca ctgatcaata agccaacctg ctgccagttt tactgaacca cccgcctggg
     3721 ggtaatttgg tgctgttgga aattgcgcca gtaatgcttt agccgtttcg gcagatacaa
     3781 cagggttttt gaagaaacta ccggcattgc cattcacttt tggatcaggg agtttggtgg
     3841 tgcgcatatg acacaccgca ttaaatactt gctgtggcgt tactgttgta ggatccagac
     3901 gagttaagtc accatacgtt agtacaggtt gccactcttt tggcagacgc agacctacgg
     3961 ctacaatggc gaagcgatcc tggtattcat gtttaaaaat actgtcgcga tagccaaaac
     4021 ggcactcttt ggcagttaag cgcacttgct tgcctgtcgc cagttcaaca caatcaacat
     4081 aagcgcaaac tcgctgtaat tctacgccat aagcaccaat attctggata ggtgatgagc
     4141 cgacacaacc aggaattaat gccagatttt ccagaccagg cataccttcc tgcaaagtgt
     4201 attttaccag gcgatgccag ttttctccgg ctcctacatg taaataccac gcatcaggtt
     4261 catcatgaat ttcgatacct ttgatccggt tgatgatcac cgtgccgcga tagtcttcca
     4321 gaaaaagtac attacttcct tcacccagaa taagaacggg ttgtccttct gcggttgcat
     4381 actgccaggc attgagtaat tgttgttcgt cttcggcaca tacaatgtgc tgagcattat
     4441 gatcaatgcc aaatgtgttc cagggtttta aggagtggtt catagctgct ttcctgatgc
     4501 aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg acgacaggaa
     4561 gagtttgtag aaacgcaaaa aggccatccg tc
//
LOCUS       DAZGOB010000230         4561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGOB010000230 DAZGOB010000000
VERSION     DAZGOB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.231"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..318)
                     /locus_tag="ACHLIF_004938"
     CDS             complement(<1..318)
                     /locus_tag="ACHLIF_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO9608765.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(616..954)
                     /gene="osmE"
                     /locus_tag="ACHLIF_004939"
     CDS             complement(616..954)
                     /gene="osmE"
                     /locus_tag="ACHLIF_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO9608766.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1156..1983
                     /gene="nadE"
                     /locus_tag="ACHLIF_004940"
     CDS             1156..1983
                     /gene="nadE"
                     /locus_tag="ACHLIF_004940"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO9608767.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2213..3100
                     /gene="cho"
                     /locus_tag="ACHLIF_004941"
     CDS             2213..3100
                     /gene="cho"
                     /locus_tag="ACHLIF_004941"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO9608768.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3060..3635)
                     /gene="ves"
                     /locus_tag="ACHLIF_004942"
     CDS             complement(3060..3635)
                     /gene="ves"
                     /locus_tag="ACHLIF_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO9608769.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3838..4323)
                     /gene="spy"
                     /locus_tag="ACHLIF_004943"
     CDS             complement(3838..4323)
                     /gene="spy"
                     /locus_tag="ACHLIF_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO9608770.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPIMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIVASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1141 a         1102 c         1102 g         1216 t
ORIGIN      
        1 atttgctgcg gcttttttaa tcgctgcaac cgcagcctta agcacgccta aaccatcgac
       61 tttgccataa agcagcgagt caattacttc aaccggtttg ttgggtaaca aacgctggat
      121 ttcgggcaac atataagcaa tctgcggccc taataacacg acatcggcat tctgaccttt
      181 ttcaccagcc agtgtttccg gaaatgcttc aataatgacc ggaacttcat atttttctgc
      241 ctgtgcgcgc atttttgata ccagtaaaga ggtagacatg cccgcagaac aaaacagata
      301 aatgtgtttc ttttccataa aactgccctc atcgacgatt atctgtcagc cagacactcc
      361 gcaagcctta acctgcttcc atgctctggg taacttgcga aaccaacatg acttttttgt
      421 ggctgaaagg agtatacggt atagacctga gcaatagtat ttctttgacc ttataaattg
      481 tttctccttg acctgtggtt atgaccctct tcacatttcg ggcaaataat tcgcgaagat
      541 aattaagaac tcaggccaaa aaaaaccggc gcaatggccg gtttccgttg ttactcaagc
      601 tttcagacga attgattact tcgcagcctg tggatcagtg tcgtattcag cacaggtctg
      661 ataaccggag ttgatgacat gtccggtatc atctaacgcg acaaagtagg tttctgcttt
      721 accatcacgt tgacccagga tgtaggtctg gcaagtaccg cgagcatgga tcatgctcac
      781 ttcagacgaa ggtttacccg caatttgtgc aacctgcgcc cggctcatgc cttttttgac
      841 gtctttcacc acaggctgta caaactggtc tttggtacga tcataagccg tacaacccgc
      901 cagcatggtt aataccgccg ctgcactcag aattcctgcc atattcttgt tcatattccg
      961 tcctcttgtt tatcagcgtg ttagataagc ctggaataca ttgggcgctt tttcaagccc
     1021 gtgaacgaaa cggctccgct ttcagaggat tcctgtatga cgttttaacc accattcagc
     1081 ccgctgtcgc ttgtcgtttc agtagcaacg ggttagcttt aaggaagttt tgtcttttct
     1141 gtctggaggg gttcaatgac attgcaacaa caaataataa aggcgctggg cgcaaaaccg
     1201 cagattaatg ctgaagagga aattcgtcgt agtgtcgatt ttctgaaaag ctacctgcaa
     1261 acttatccgt tcattaaatc actggtgctc gggatcagcg gcggtcagga ctccacgctt
     1321 gccggaaagc tgtgccagat ggcgattaat gagctgcgcc aggaaactgg caacgaatca
     1381 ctgcaattta ttgccgtacg cctgccctat ggtgttcagg ctgacgaaca agattgccag
     1441 gatgccattg cctttattca accggatcgc gtattaaccg ttaatatcaa gggcgcggta
     1501 ttggctagcg agcaggcatt gcgggaagca ggcattgaac tgagcgattt tgtccgtggc
     1561 aatgaaaaag cgcgtgagcg gatgaaagca caatatagca ttgcgggtat gaccagcggt
     1621 gtcgtggtgg gcaccgatca tgcagcagaa gccattaccg gattcttcac taaatatggt
     1681 gacggcggta cggatattaa tccgctgtat cgtctcaaca aacgccaggg taaacagttg
     1741 ctgacggcat taggttgccc ggaacacctt tataagaaag ccccaacggc cgatctggaa
     1801 gatgatcgcc cttctctgcc agatgaagtg gcactcggcg tgacctatga caatatcgac
     1861 gactatctgg aagggaaaaa cgtacctgaa caggtcgcca gaacaataga gaactggtat
     1921 ctgaaaaccg aacataaacg ccgtccgcca attaccgttt tcgatgattt ctggaaaaag
     1981 taataatttg cacataatgg tttgtgcgaa aaagagtaat ttgttcacgc cggatgcggc
     2041 gtgaacgcct tattcgacct acaaaactat gcaaattcaa tatattgcag gagtcgaata
     2101 ggcctgacag gcgtagcacg tcagacggtg taacctttgt catcgacccg cctctttttt
     2161 aatcgcttcc cgcctgttac actggataga taaccagcat tcggagtcaa cagtggtacg
     2221 gcgtttaact tctccgcggc tcgaatttga agctgcggca atttatgaat atcccgaaca
     2281 tttacgttca ttccttaatg acttacccac ccgaccaggg gtgtatctgt ttcatggcga
     2341 aagtgacacc atgccgctct atatcggcaa aagcgttaat atccgcagtc gggtgctttc
     2401 tcatttacgc accccggatg aagccgccat gctacggcaa tcccggcgaa ttagctggat
     2461 ctgcaccgca ggcgaaatcg gcgctctgct ccttgaagcg cgattaatca aagaacaaca
     2521 gccgctgttt aataaacggt tgcgccgcaa tcgccagctc tgtgccctgc aattaaatga
     2581 aaagcgcgtc gatgtggtgt atgccaaaga ggtggatttt tcacgagccc ccaacctgtt
     2641 tggcctgttt gccaataggc gcgcagcttt gcaagcattg cagagcatcg ctgatgaaca
     2701 aaaactttgt tatggcctgc tgggactgga accgttaagt cgcggtcgtg catgttttcg
     2761 ttcagcgcta aaacgttgcg ccggagcatg ctgcggtaaa gagagccatg aggaacatgc
     2821 gctacgcttg cgccagtctc tggagcgttt gcgggtggtg tgttggcctt ggcaaggggc
     2881 ggtggcgctg aaagaacagc acccggaaat gactcaatat catattattc aaaactggct
     2941 gtggctgggg gcggttaatt cgctggaaga cgcgacaacg ttaattcgga cacccgccgg
     3001 gtttgatcac gacggttata aaattctttg taagccgctg ctttccggta actatgaaat
     3061 tactgaactt gatccggcga atgaccagcg agccagttga tttcactgaa caacagttta
     3121 ccttgcggtt gtaaaagccg cagggtgtgc ctgccatcga accagcacgc gccctggtcg
     3181 gtagtgagca gtttgtcgcc cagttgccac gccccgttaa tcacaaacac taccccgccg
     3241 cgtgagccaa aggtagtaaa cgtacgttcg gcaattctga ctttcgcctt acagacatcc
     3301 aggcgcgtca taatattgaa atccatcgac atctgccctg ccgtcagttt cgcttttacc
     3361 acctggtccg ccgcaaaggc aaaaggctgt aacggtttta aggtatggtt aaagtggtct
     3421 gcgctttcaa ggaacatctc gccgccttcc agcaacgtca ctatcctttc cataccggga
     3481 aacaaagaaa actccccatt cgccgcgatg gatgcaatgc tggcacgcca gtaaaaatca
     3541 cgttttgccg gtgggaaagt gcaaatttcg cgcgtttcac cggcagcatt tcgccacaga
     3601 ttcaccgaca ttttacgcat atcaaagtat tccatactcg ctcccggccc gttcttttca
     3661 ttgttatcgt tcaggtaacc gtggatggtt atcggcaact cgggtgattt acgttagtgg
     3721 tgatcaggct ttttttacaa ctgtttgaga agagaaaaga aaaccgccga tcctgtccac
     3781 cgcattactg caaggtagtg gacaagaccg gcggtcttaa gttttttggc tgaaagatta
     3841 ttcagcagtt gcaggcattt taccttttgc cgctggacgt tctgtcagac gcttctcaaa
     3901 attagcatta aattgctttt tctgttccgg cgtcaggatg ttgtaaattt tgttctgggt
     3961 ttccatgtgc gccagcatgt tagctttgcg ctgttcttcc atttttgcga tctgcgcttc
     4021 agcttttgct ttatcgaagg tatcgctggc aacgatgtca tgcattgcgc ggcgttcttc
     4081 cagcggcgga cgtttcatct ggtcacgctg gcctttcatg atttcgcgga tctgctgttt
     4141 ctgcgcgtcg gtcaggttca ggtctttgaa catcatgtcc tgatgcggac cgaacttgcc
     4201 tttgtggtgc ataatcggct tcgcgtcagc cggtgctgcg gtagtggtgt ctgcggcatg
     4261 ggccaggtta gccgcgccaa gagccagggt agaggcaaca aacagtgcag ttaatttacg
     4321 catattctat atccttcctt tcagttattt attacggctt tcttaagtag cgtgccgtgt
     4381 tgacgagatt aactttactg actttagcgt caattaatca gagcaacggt aaaacaatga
     4441 aagtgtaaaa aacacttttg cgccaattat ggagaaaaaa agaaaatttg atggagagtg
     4501 atgagagaat attacaacac gatgattttg cagagattat gaagaactat accggatgac
     4561 t
//
LOCUS       DAZGOB010000231         4550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGOB010000231 DAZGOB010000000
VERSION     DAZGOB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.232"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            83..1663
                     /locus_tag="ACHLIF_004944"
     CDS             83..1663
                     /locus_tag="ACHLIF_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO9608771.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMGK
                     FIPVLAIPLIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
     gene            1663..2871
                     /locus_tag="ACHLIF_004945"
     CDS             1663..2871
                     /locus_tag="ACHLIF_004945"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFO9608772.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNVACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            2840..3436
                     /locus_tag="ACHLIF_004946"
     CDS             2840..3436
                     /locus_tag="ACHLIF_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO9608773.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            3508..4455
                     /locus_tag="ACHLIF_004947"
     CDS             3508..4455
                     /locus_tag="ACHLIF_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO9608774.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
BASE COUNT         1262 a          831 c         1060 g         1397 t
ORIGIN      
        1 ctggaagaga aactatatct ttctattcgc atcaaagtat taatggacta attatacata
       61 acattttcat gagataacca gtatgaaaca aaagaaagcc tggagttttt ttcagagcct
      121 ggggaaggca tttatgtatc ccatcgctct gttaagtgta tgtggtatga tgttagggct
      181 gggaagtggt ttagccagcg atgatatggg aaaattcatt ccagtgttgg ctattccgtt
      241 aattaaaacc atacttgatt ttattgttag ccttggtttg tttgccttcg ttaatttacc
      301 tgtattgttt gcgatagcta tacctttggg attattaaaa gagaaagatg ataaagccta
      361 tggcgctttt tctggcttaa ttggctttat ggcgatgcat cttggaacga acttttatct
      421 taaacagcac gacttattgg tagctgctga ccaaatgtcg acacatgggc aaaccatcat
      481 actcgggatc cagtcctaca ataccagcgt gttgggtggg attgtggcag ggttattagt
      541 cgccagcatg tataaaaaga tcgttaactt acgtattcct gaatcattag ggttttatag
      601 tggcccacgc cttgtgccta tcattacact gattgtgatg agtggatttg gtctgttcat
      661 tccttttatc tggccgccat ttttcaatct tttcatgctc attggtcact ggatttcaac
      721 ttccggtcct gttggttatt tcttctatgc tgttgccgaa cgcgtgacga taccttttgg
      781 cttaaatcac ttggtgacat cagttttccg ctttacccca atcggcggat cggcagtgat
      841 tggcggtgaa gagtattacg gcaccctgaa catgtttatg gcatacgtca aagagaatgc
      901 ggtcattccg ctggatttgg cggggaaaat ggagcagggc aaactgatga ttcagtatgg
      961 tctggctgga gccgcgctgg cgatgtatcg cactgctcat gctcaaaaca gaagggctat
     1021 caaagcattg ctgatttccg gtgtgctcac ggtaattatt ggcggcgtca gcgagccgat
     1081 tgaatttctg ttcttatttg tcagcccgct gttgtttgtc ttccatgcct ttatgaatgg
     1141 attggctaac atggttctgc catatatggg cgtgaagatg ggctttactg gcgatctgat
     1201 tcaatttatt agctttggtg tcttacgtgg cacaagaacg ggatggccat tagcggtatg
     1261 tgttgaagtg gcttatttct tcatttatta ctttgtgttc cgttggacca ttctcaaatt
     1321 taacctgatg actgtaggcc gtgaagagtc cagtcctgtc acgctgaacg ctcacgaaga
     1381 aactgcaata gctaacatcc cagttcatga taaatcagag ttgcaggcag cggagcaaat
     1441 ggttaaggct ctgggtggta aagaaaatat taaatcactg gataattgtg taactcgttt
     1501 gcgtttaact atcgcggata tgggattgat agatgaggct gcaataaaaa gggccggagg
     1561 aattgcggtt gtgaaacttg atcaaaatac cttacaagtc attatcggta caaaagtcat
     1621 cgccctacgt cgggatatgg ataattacat ggggatatgt tgatggatat ttttaatacc
     1681 tcaatttctc gtaaaggaac atattgcaca caatgggact tttgtgaaga cagatttggt
     1741 gtaaaagatg tattaccatt ctctatttca gatatggatc ttccgatacc ggaagctatc
     1801 atcagaacat taaaaaaacg tctggaacac ccgattttag ggtacagtcg ttggcagcat
     1861 gatgactatc tgaattcgat agtcaattgg tatcatcaac aatataaaac agagattcaa
     1921 ccagaatgga tcacctatag cccaagcgtg atgtattcaa tatctaaggc aatagaaata
     1981 ttgacattga atggagataa tattctcatt ttcacgcctg tttataacgc attttttgat
     2041 gtgattaagc ataatggtcg aaacatgcta acaacctctt tgatgaaaga aaatgggaga
     2101 ggctacgtta tcaactggca agactttgag cttaaaatta aaagcgcgaa gatggtgtta
     2161 ttatgtaatc ctcataaccc gacaggaacg gtctggtcgg aagacgatct tcacagaatc
     2221 gcagcatgct gtgcaaggca taatatttgg ttatgttcag atgaaataca tagtgatttt
     2281 gtttttaatc atagtttcac gtcagcgcta acaataaaag atgacaaagt ggtggtgttt
     2341 aattcgattt cgaaaacatt taatgttcct gcgctaaccg gatcatatat gatttcaact
     2401 gacgataatt ttaatcataa gttcagaacg atatcgaggt atcgcgattt cgtaaactcc
     2461 ccatcagtac tgaatgtaat tgcgaccatt gttgcctata atgaatgtga agaatggtta
     2521 aagtcgttaa aggtacatat tgcctctaac attcaattta ccttgcagta tttaaatgaa
     2581 aatattccgg aattgatcgt atggccagct gagggctgct atttttcgtg ggttgattgc
     2641 tcggcccttg gttatccctt tgataaattt taccgtcgat taattaacga agggagagta
     2701 ggtattatgg cggggcatgt ttatggtact gaaggagagg gctatctgcg tcttaatgtg
     2761 gcctgcagtc gagaaaaatt atatatgggg cttgcgcgcc ttgcgagtgt aattaaaaat
     2821 attaatcagg aaaaagaaaa tgaacaatac ggatcttatc catcttatta aacgttttat
     2881 gcgtaacgaa cataaagcag tagaagaggt tattgattcc cccctttcgg aagtcgcgaa
     2941 tttaattaaa atattacaat cttgccaggg aaaagttgtt tttattggtg ttgggaaatc
     3001 tggcattatt gccagaaaac ttgctgcaac atttgccagc accggaactc cctcgttttt
     3061 tgttcacggt acggaagcgg tacacggcga ccttgggatg gtggcgaaag acgatgttgt
     3121 tattcttatt tcaaacagtg gtgagacggc ggaaatcctg gcgacactgc ctagtttgaa
     3181 aaaaatgggt aattatttga tctcttttac ccgcagtcat cactcatcgc ttgcgataag
     3241 ttgtgattta tctgttgaaa tccccgtcaa aagtgaggcg gataatttag gtttagcacc
     3301 gtcatgctcg tctactgttg ttctggttgt tggtgatgcg gtggcgcttg cactctctga
     3361 attgaaaaaa tttactcgtg ctgatttcgg cttatatcat ccaggaggtg cactcggcat
     3421 taaagcaaat tcataatttc aaagtataaa tacagtaacc ggtcacgcca tcttttgggg
     3481 tggcgttcat tgaagtgagt gtcagctatg aaaaatgttc tggtcaccgt ccccagcttt
     3541 tcagcacgct gtgtttccgc cagcaagttg ctaagagaga ataattttaa tcttattatt
     3601 aagaataatg tggagcatct gcttaaatca gaatccactg cattacgaga atctatttgc
     3661 gcggtcatcg ccggaaaaga ttgttatcag gcggacacgc tatcattgct gcctggtgtg
     3721 aaaataatat cgagattcgg gacggggata gataatattg atcttcgtgc agcacaacaa
     3781 tccggtattg tagtcaataa tgcggttggg attaattcaa atgctgtcgc ggaatttatc
     3841 attggactta tcttcgcgag catgagaaat attccaggca gctatcatgc gatgcaaaat
     3901 ggctactggg gtgagtcgca tggctatgaa ttacaaggca aacggattgg catggttggc
     3961 tacggcaata taggtaaaac cctggcaaaa aggttatccg gttttgatgt tgagctttta
     4021 gcttatgaca aacaacccga ttatcaggtt gctgacaaag ttggagttca gtttgtatca
     4081 attgaagata tctttatgca gtcgcatgtc atcattgttc ttttgccttt ttctcctgag
     4141 ctggagaact tcattagtca taaatatttg tcgatgatgc gcaatggtgc tctaatcatc
     4201 aatgccgcaa ggggaaagtt gctggatgag ggcgctttac tacaggtgat tgaagaacga
     4261 aatgtgtttg cggcgctgga tgtgtttagc tctgaaccgt tggcgcaatt tagcccactg
     4321 ctacatgcca aaaatatcat tacaacgccg catattgcgg ccgccaccgt tgagtcatac
     4381 cagcaaaccg gtacacacgt tgcccagtcg ataattgatt acttcgcagg aagggagata
     4441 aaaaacgtgt tgtgagttca gcgaacgaag tttttaaata caaaaaaaac cgcaatttgt
     4501 ggaacacatt gcggtttatc agcattctaa gtattggttg cgggggccgg
//
LOCUS       DAZGOB010000232         4378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGOB010000232 DAZGOB010000000
VERSION     DAZGOB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.233"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(49..125)
                     /locus_tag="ACHLIF_004948"
     tRNA            complement(49..125)
                     /locus_tag="ACHLIF_004948"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Arg,seq:tct)
     gene            complement(223..298)
                     /locus_tag="ACHLIF_004949"
     tRNA            complement(223..298)
                     /locus_tag="ACHLIF_004949"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(263..265),aa:Ile,seq:cat)
     gene            complement(348..1397)
                     /locus_tag="ACHLIF_004950"
     CDS             complement(348..1397)
                     /locus_tag="ACHLIF_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO9608775.1"
                     /translation="MINTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWEGDDDYLKWLDQCLAQFWRVLKPAGSLYLFFGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYVAQGRELKQHVMA
                     PLISYFRDARKSLGITSKQIAEATGKKNMASHWFGTSQWQLPNEGNYNKLQALFARVA
                     AEKHQRGELEKPHHQLVSTYSELNRQYTELLSEYKNLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIINASSRPGDLVADFFMGSGSTVKAAMALGRCAIGVELET
                     GRFEQTVREVQDLIV"
     gene            complement(1548..1745)
                     /locus_tag="ACHLIF_004951"
     CDS             complement(1548..1745)
                     /locus_tag="ACHLIF_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9608776.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(1977..2519)
                     /locus_tag="ACHLIF_004952"
     CDS             complement(1977..2519)
                     /locus_tag="ACHLIF_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO9608777.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGKLRMWGRWSYIGGGKPGN
                     MFNQLLASKKLTKTAINEALRRIRESGIDKPELEAFLREMIAGRQKSWLSHCTDAEAL
                     RIDGVISKALARYPGLIDILRQRYEGRGMSKRKMAELLNEVHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKMS"
     gene            complement(2528..2893)
                     /locus_tag="ACHLIF_004953"
     CDS             complement(2528..2893)
                     /locus_tag="ACHLIF_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9608778.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQRVPGGRLGVKIYKIESE"
     gene            complement(2894..3949)
                     /locus_tag="ACHLIF_004954"
     CDS             complement(2894..3949)
                     /locus_tag="ACHLIF_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9608779.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRNQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWATINNVADALPETVLRRSLGLRAEKIRSVYRESDIVPGEQTATSMLKQR
                     TKNIALLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPRREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            complement(3951..4223)
                     /locus_tag="ACHLIF_004955"
     CDS             complement(3951..4223)
                     /locus_tag="ACHLIF_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608780.1"
                     /translation="MVHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            4170..4349
                     /locus_tag="ACHLIF_004956"
     CDS             4170..4349
                     /locus_tag="ACHLIF_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608781.1"
                     /translation="MPDGKVGIRIVKFGFVHHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
BASE COUNT         1022 a         1187 c         1026 g         1143 t
ORIGIN      
        1 tatattccag tataaggctc tgccggaaga aatctggaag aatgaatatg gcgcgttgta
       61 ctggattcga accagtgacc gattgcttag aaggcaattg ctctgtccgg ctgagctaac
      121 aacgcataat gcagataatg gattgccatc ggggacccgg gccccacaca gccagtttcg
      181 aaagctgacg ctctctgccg atgagctaat ggcggtatgt gatggtggcc cttgctggat
      241 ttgaaccagc gacctggcga ttatgagtcg ctcgctctca ccactgagct aaagggccgg
      301 tagcagaata ataatggtgc gtaattaatt ctgcaatccc atccgtttca aacgattaaa
      361 tcctgaactt ccctgactgt ctgttcaaaa cgtcctgtct ccagctcaac accaatcgca
      421 caacgcccca gtgccatcgc cgcttttacc gttgaacctg aacccataaa aaaatctgca
      481 accaggtctc ccggacgact gctcgcgttg attatctgct gcagcatttc tgccggtttt
      541 tcgcacggat gtttccctgg atagtactgc accggtttat gcgtccagac atcggtgtac
      601 ggaacctgcg ccgtcacacc gaaataccgc cgcaaatttt tatattcact cagcagttcc
      661 gtatactgcc ggttcagctc actgtatgtg ctgaccagct ggtggtgtgg cttttccagt
      721 tccccgcgct gatgtttttc tgccgcaaca cgcgcaaaca acgcctgcaa tttgttgtaa
      781 ttaccctcgt tcggtaactg ccactgactg gtaccaaacc agtgcgaagc catgtttttc
      841 tttccggtgg cttccgctat ctgttttgac gttattccca gtgatttacg cgcatcacga
      901 aagtaagaaa tcagcggggc catgacgtgc tgttttagct cgcgcccctg tgccacataa
      961 ccatcatctt tcgggcgata cggtccctga taatgttctg caaacagaat gcgctctgtt
     1021 gccggaaaat acgcccgcag actttcctta ttgcacccgt tccagcgtcc ggacggcttc
     1081 gcccagataa tatggttcag cacattaaag cgctcacgca tcatgatttc ggtgtcagat
     1141 gccaggcgat gaccaaagaa caggtaaaga cttccggcag gcttcagtac ccgccagaac
     1201 tgcgccagac actggtccag ccatttcagg taatcatcgt cgccctccca ctggttatcc
     1261 cagccctcgg gcttcacttt aaagtatggc gggtctgtga ctatcagatc gacagagttt
     1321 tccggtaagg tctggataaa ttccaggcaa tcagcgttga ttaactcaca actggatatt
     1381 tttacagtat taatcataga tcaataagca cttctctgat aggctcatac cgcttttgcg
     1441 caaagcagat gggcctgagg tttgcttgtg accccaacgc atgagcagat ggctggcagg
     1501 tgccgctaac acccaccagc cgcccattac cacaaattaa aaagccttca ctgcggaagg
     1561 cgtctgtaac aaccgaactg ataatctgcc agacccgcca taacaagctg agtcagtatt
     1621 aactggcagc gttcgcgtga aaggtaagta ttctgcgcaa tttccccgac ggtcgccggt
     1681 tcggtgacgc ttaattcatt aaacaccact ctggcggttt cggtcatatc ctgctgtttt
     1741 agcatgcttt tttccctttt ccggttaacg tgacatacca atacctcttg tcgaaaaagc
     1801 cagcaagctg aaagaccagt attcacaact accagcgcgt ttaatgttct gtgccgtttt
     1861 tcagacataa aaaaacccgc ataaagcggg ttctttcagg tgtccatgtc tgctattcgc
     1921 ctcgcggtat agctttgcga agcgtagctg gattgaaaca gtttatgcgt aaaaaatcaa
     1981 gacatttttt gagcaaacga ttctcgcata gggatgtata gcgcatattc agcaacagcc
     2041 aaccaattag caattcgctt ttcgcatgta ctaaaacacc attccgggtg cacctcattc
     2101 aacaattcag ccattttgcg tttactcatc ccccgccctt cgtacctttg ccgcaggata
     2161 tcaatcaatc caggataacg tgcaagcgct ttacttatca ccccatcaat gcgtaacgcc
     2221 tctgcatcag tacagtgaga caaccagctc ttctgtctgc cagcgatcat ctctcgcaag
     2281 aatgcttcca gctctggttt atcaatccct gactccctga ttctacgcag ggcttcattg
     2341 attgcggttt ttgtcagttt tttggatgcc agcaactgat tgaacatatt tcctggtttg
     2401 ccaccaccta tatacgacca acgcccccac atccgtaatt tcccctggat ccagacggct
     2461 tccagcgttt ttagacgtaa atgctcgccg cttttgcctg taatttccgg gtatatcata
     2521 tttacgatca ctcactctca attttgtaaa tcttcacgcc cagccgtccc ccaggaacgc
     2581 gctgaccgcg cacaatattg atttcatcaa actgctcgtc gtctatgaga agtccggcat
     2641 gcgtcagcgc atccagtggt gctttcagga tattgtccag gtcacgacga cgtttatccg
     2701 gtggctctgc aataatcttt atcgccagcc ttccggacag gtttaatttc agccgctgct
     2761 ggcgaacaat aagcgccaca tcacggcgat aacgctcacc ggcttttgat acaaaatatg
     2821 tgctgccacg acgtcgccag taagtgttca ccgttggcgg gtaaggcaaa acaaattcta
     2881 tgcgttcagt cattcatgct ttccacttca ggacacccga atttctcgcg tgcattaaaa
     2941 aacgaatcag caacaacagc tggctgccgt gtttttcttc aaaatctttt accccggcgt
     3001 gtagttcgct atggcattta cggcacagcg gaataacaaa caaatcatca gcctttgttc
     3061 ccatccctcc cagtccatga ccaatgatgt gatgcggatc atctgcctga ttaccacacg
     3121 tcatgcattt ctgcgttttt acccaacgcg tgtatacagg catctcttcc cgtcgtggtt
     3181 tctggcgctg gagatactga gccggtgact ccggatcaac ggcaatgctt accaccgtct
     3241 tttcctgtgg cgggttttgc tggtgggcgt gaggcagtag cgcaatattt tttgtgcgct
     3301 gcttcagcat gctggtggcg gtctgctctc ccggcacgat gtcgctctcg cggtatactg
     3361 agcgaatttt ttccgcgcgt aatcccagtg aacgacgtaa caccgtctcc ggtagtgcgt
     3421 ccgctacgtt atttatggtt gcccaccagg ataattcagc cagcgataat tcccgctcct
     3481 gcgtgccatt cattgcgtgg cggatgacat caatcatcca tgctgacagg ttttggtgag
     3541 caagctgccc gagtgattcg gaagtctggt tacgcagctg gttgtcgcag tgccagcaca
     3601 acaccatcgc gccggtaccg taacgatgta tgacggtttc actgtgatgg tagtcaccat
     3661 gaggccactg gcaggattta atgtggcgta acagccagtc agacaatgca ccagcaccac
     3721 cagcagcacg aatcacccgc tcatcgctga aaaatggcag taatgattta tcctccgcca
     3781 gcggctggcg aacagcagga acgactccgg acggcagacc gcgcatgctt ttcggttccg
     3841 gctccaccag aactcgaggg ttatgaaata cctgcatgga ttcacggccc ggttttagca
     3901 ccaccagccc aagttccggt accggaacag gtcgaagtaa tacccgcacg ttacctccag
     3961 atgcgttgct ggaatgtgcg ggacggacgc ggtgggcgtt cggaataagg gagcctgaca
     4021 tagattatcc agtgacgata atcgaggctg agggctttct taatctcgta tccgcgtctg
     4081 cggtagttat gaattagcca ttcggcctgt tcttcagtac atggtgggtg ttggtaccag
     4141 tcggttttaa atgcgtgtga acgccgccca tgccggatgg caaggtcggt atcagaattg
     4201 tgaaatttgg ttttgtgcac catctgtttt ctctgctggc gcagcaggtg tcaggtgttc
     4261 aggctgacgt gcgaattgta aaccagaatg ccaggaaaaa acaaaacccg ccgaagcggg
     4321 ttaagtgcgg gtgcgttgag gatgcctgac tcatcagagg tggcgaggga tttctccc
//
LOCUS       DAZGOB010000233         4369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGOB010000233 DAZGOB010000000
VERSION     DAZGOB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.234"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            12..365
                     /gene="cesL"
                     /locus_tag="ACHLIF_004957"
     CDS             12..365
                     /gene="cesL"
                     /locus_tag="ACHLIF_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFO9608782.1"
                     /translation="MNLLIKRNVEEFLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDSN
                     VFITFALEYNENAFFSFFNALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFSLMNTF
                     QQHVQIIERVRRMTS"
     gene            362..2389
                     /gene="escV"
                     /locus_tag="ACHLIF_004958"
     CDS             362..2389
                     /gene="escV"
                     /locus_tag="ACHLIF_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO9608783.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            2373..3713
                     /gene="escN"
                     /locus_tag="ACHLIF_004959"
     CDS             2373..3713
                     /gene="escN"
                     /locus_tag="ACHLIF_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO9608784.1"
                     /translation="MILEHDSVLERYPRIQKVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVKSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            3773..4093
                     /locus_tag="ACHLIF_004960"
     CDS             3773..4093
                     /locus_tag="ACHLIF_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO9608785.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKEDLQHVNKSIEKLSFAIKE
                     HYFD"
     gene            4086..>4369
                     /locus_tag="ACHLIF_004961"
     CDS             4086..>4369
                     /locus_tag="ACHLIF_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFO9608786.1"
                     /translation="MTSISLKRNLIEPVSFQPSDDWPQGEESFNDIFNRIKKRTPDTS
                     FIRMASLWKRLFYAPGQRRRYFEQGERSFSIICGKLRGIVLTIKCSNGTI"
BASE COUNT         1244 a          772 c          995 g         1358 t
ORIGIN      
        1 agagcctgca tatgaatctt ttaattaaaa gaaacgtaga agagttttta agattattag
       61 gaaatgattt ttatttgttt gataatcgtg tggaaattga ttttaatggt ttttcttttt
      121 ttattgaaat aattgatagt aatgtgttta ttacgtttgc cttagagtat aacgaaaatg
      181 cgtttttttc ttttttcaat gcccttgccc ctgaacggac gcagggagtg attgaacata
      241 tttttgtcta cgataacaaa ttgtgcttaa gttgtttgtt aaccaatatt gatgtgtttt
      301 ctctaatgaa tacttttcag cagcatgtgc agattattga acgagttcgc aggatgacat
      361 catgaataaa ctcttaaata tatttaaaaa agcagagtca tatcacgatc ttattctggc
      421 tctcttcttc tttatggctg taatgatgat gattattcca ttgccaacag ttgtggtgga
      481 tatcattatc gcgattaata tatcgacagc gttgctttta ttaatgctct caatctatat
      541 aaaaaatcct cttgagctaa cttcttttcc tacaatcctg ttgattacga cgttgatgcg
      601 cctgtcgctc agtgttagta caaccagact tattctgctg catcacgatg cgggtgacat
      661 tatttactcc ttcggtaact ttgttgttgg cggtaatatt gttgttggcc tggttatttt
      721 taccattatc accattgtac aatttatggt tattacaaaa ggggctgagc gtgttgctga
      781 agtgagtgct cgtttttccc ttgatggtat gccaggaaaa caaatgagta ttgatggtga
      841 tatgcgcgca ggcgttattg atccgcttga ggcaaaagtt cttcgctccc gagtgcaaaa
      901 ggaaagccag ttttatggtt caatggacgg ggcgatgaag tttgtaaaag gggacgctat
      961 tgccggtatc atcattgttt tagttaacct gtttggtggc gtgctcattg gaatgtggca
     1021 atttgacatg ccatttagtg aagcacttag cctgttttct gtattgtctg tcggtgatgc
     1081 tttggttgcc cagatccctg cgcttattat ttccgtcact gcgggggttg ttgttactcg
     1141 tgttcccggt gaaagcgaaa aagaagaaaa ccttgcaggt gatattgttc aacaggtttc
     1201 tgttaatagt cgcccttttt taatcagtgc cgcactcatg atcgtaatgg cgattattcc
     1261 tggctttcct acattggtct tcttgtttct ggcggtttgc ctgttgggta tcgcctggaa
     1321 attacaaaag aaaagaacgt ttggaaccgg aaataataag gacgctatgg gagctgattt
     1381 gtctaatagt caaaatatct caccgggtgc cgagccgttg attttaaact taagtagtaa
     1441 tatttatagc tcagatatta cacagcagat tgaggttatg cgttggaatt tctttgagga
     1501 aagtggaatt ccattgccta agattattgt taatccagtt aaaaataatg atagcgctat
     1561 agaatttttg ctctatcaag agtcaatata caaagatact cttacagatg atactgttta
     1621 ttttgaggct gggcatgcag agatatcatt tgaatttgtc caggaaaagc tttcggctaa
     1681 ctctatcgtg tataaaacga atgaggctaa ccaacaacta gctcacgtct caggtatgga
     1741 tgtttatgca aaaacaaatg acaagataac gtttttgcta aagaaacttg tattgtctaa
     1801 tgccaaagag ttcatcggcg tacaagaaac gcgttatctg atggacatca tggagaggaa
     1861 atataacgaa cttgtgaaag agcttcagcg tcagcttggt ctgagcaaga ttgtggatat
     1921 tcttcagcgt ctcgtagagg aaaatatctc aattagagat ctacgaacta tttttgaaac
     1981 gcttattttt tggtccacaa aagaaaaaga tgtggttatc ttgtgcgaat acgttcgtat
     2041 cgccctccgt cggcatattt taggtcgcta tagcgttcgc ggtacacttc tgaatgtctg
     2101 gcttattggc tctgatattg aaaatgagtt acgagagtcg atcagacaaa cgtcatcagg
     2161 ttcgtatttg aatatctcac cggagcgaag tgagcagatt attggcttct taaaaaatat
     2221 tgtgaatcca acggggaatg gcgtcattct gaccgcttta gatatcaggc gctatgtgaa
     2281 gaaaatgatt gaaggttcgt tcccgtcagt ccccgtgctc tcttttcagg aagttgggaa
     2341 taatatcgaa cttaaagtat taggaacggt aaatgatttt agagcatgat tctgtattgg
     2401 aaagataccc acgtatccag aaagtgctca atagcactgt gccgacatta tcattaaatt
     2461 cgtctaccag atatgaaggc aagattacca atatcggcgg gacgattatc aaggcgcgtt
     2521 taccaaaagc gcgtattggt gccttttaca agatagaacc cagtcaacgt ttagctgagg
     2581 tgatcgctat tgatgaagat gaggttttcc tgctgccttt tgagcatatt tccggcatgt
     2641 attgtggcca gtggctaagt tatcaggggg aagagttcaa gattcgtgtt ggggacgaac
     2701 tccttgggcg actggttgat ggtataggca gaccaatggg gagcaatatt actgcacctt
     2761 atcttccgtt tgaacgcagt ttgtatgctg aaccccccga tcctttatta aggcaggtta
     2821 ttgatcagcc atttacgctt ggcgtcaggg caatagatgg tttacttact tgcggtattg
     2881 ggcaacgcat cggtattttc gcggggtccg gcgttggtaa aagtacgctt ttgggaatga
     2941 tttgtaatgg cgcatccgcg gatattattg tccttgccct tatcggcgag cgtggtcgcg
     3001 aagtaaatga attcctcgcg cttttacctc aatccacgct ttctaaatgt gtactggtcg
     3061 tcacaacgtc agaccgtccc gcgctggaaa gaatgaaggc cgcatttacg gcgacaacca
     3121 ttgcagagtt cttccgggat cagggcaaaa atgtattatt aatgatggat tccgtaactc
     3181 gttatgcccg agccgcgcgt gatgttgggc ttgcatcggg agagcctgat gtaagagggg
     3241 gattccctcc gagtgttttt tcctcgttac ccaaattatt agagcgtgcg ggacctgcgc
     3301 caaaaggttc aataactgcg atttatacgg tgctgttaga aagcgataat gtgaatgatc
     3361 ccatcggtga tgaagttcgc tctattcttg atggacatat tgtgcttacc cgagaacttg
     3421 cagaggaaaa ccatttccct gcaattgata ttggtttaag cgccagtcgt gttatgcata
     3481 acgttgttaa atcggaacat ttacgtgcag cggctgaatg caaaaagctt attgcaacct
     3541 ataagaatat tgaactgctt attcgtattg gtgagtacac gatggggcaa gatcctgaag
     3601 cggataaggc aataaaaaac aggaaagcaa ttcagagctt tatacaacaa agcaccaaag
     3661 atatcagtag ttacgaaaaa acgattgaaa gcctattcaa agtggttgcc tgaatatgtt
     3721 ggacagaatt ttatctattc gtaaaagcag agcgaatcga ttgagagaat caatggctaa
     3781 gattaactct cagataaaag aggtggatgg taaactcgat gactgcgagc aagccattaa
     3841 agagtcgatt gcctctaagc aggcgtattg tgcatcgctg gtgaatctgg ataaagtgtc
     3901 gttgtataaa taccagatta aaaacaatgc atttgatgag caaaagcagc gattatatga
     3961 gaaaaaatcc tcactttcta aagaaaagcg ttcattgctc gattcacaaa aacgaacaaa
     4021 agaggatcta caacatgtta ataaatcaat agagaaatta tcttttgcca taaaggaaca
     4081 ttactttgac tagcatttct ctaaaaagaa atttgataga gcctgtttct tttcagccgt
     4141 cagatgattg gccgcaggga gaagaaagtt ttaatgatat ttttaatcgc ataaaaaaaa
     4201 gaacacctga cacttcgttc atacgaatgg cttctttatg gaaacgattg ttctacgctc
     4261 ctggacagag gcgacgctac tttgaacaag gagagcgttc atttagtatt atttgtggaa
     4321 agttacgcgg aattgtttta acaataaaat gtagtaatgg aaccattta
//
LOCUS       DAZGOB010000234         4343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGOB010000234 DAZGOB010000000
VERSION     DAZGOB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.235"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            56..1309
                     /gene="ynfM"
                     /locus_tag="ACHLIF_004962"
     CDS             56..1309
                     /gene="ynfM"
                     /locus_tag="ACHLIF_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9608787.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1358..1477)
                     /locus_tag="ACHLIF_004963"
     CDS             complement(1358..1477)
                     /locus_tag="ACHLIF_004963"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO9608788.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            1733..2041
                     /gene="asr"
                     /locus_tag="ACHLIF_004964"
     CDS             1733..2041
                     /gene="asr"
                     /locus_tag="ACHLIF_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO9608789.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            2134..2217
                     /locus_tag="ACHLIF_004965"
     CDS             2134..2217
                     /locus_tag="ACHLIF_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608790.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2317..3138
                     /gene="ydgD"
                     /locus_tag="ACHLIF_004966"
     CDS             2317..3138
                     /gene="ydgD"
                     /locus_tag="ACHLIF_004966"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO9608791.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3177..3506)
                     /gene="mdtI"
                     /locus_tag="ACHLIF_004967"
     CDS             complement(3177..3506)
                     /gene="mdtI"
                     /locus_tag="ACHLIF_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO9608792.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3493..3858)
                     /gene="mdtJ"
                     /locus_tag="ACHLIF_004968"
     CDS             complement(3493..3858)
                     /gene="mdtJ"
                     /locus_tag="ACHLIF_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO9608793.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            4270..>4343
                     /locus_tag="ACHLIF_004969"
     CDS             4270..>4343
                     /locus_tag="ACHLIF_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608794.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIIL"
BASE COUNT         1102 a         1074 c         1037 g         1130 t
ORIGIN      
        1 ttttcctaac ctatgtataa gcctgatcca caggcatatt tagcaaggat ttcaagtgag
       61 ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca
      121 gccaaatcaa tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt
      181 ctctgccgga ctggcaacat ttgcacttct ctattgtgtg cagcctatcc ttccggtgct
      241 ttcgcaggag tttggcttaa cgcccgcgaa cagtagtatt tcactgtcca tttccacggc
      301 gatgttggct attggtttgc tgtttactgg ccccctatcc gatgccattg gtcgcaaacc
      361 agtgatggtc acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac
      421 cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtggc
      481 agctgttggc atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcgat
      541 ggggttgtat atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagtggtgt
      601 cttcacggac tttttcaact ggcgaattgc tctggcggca atcggttgtt tcgcgctagc
      661 ctcggcgttg atgttctgga aaatcctccc tgaatcacgc cattttcgcc cgacttcgct
      721 gcgacctaaa acgttgttta tcaactttcg tctgcactgg cgtgaccggg gattaccgtt
      781 attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg
      841 atatcggttg atgctctccc cctggcatgt cagtcaggcc gtggttggct tattatcgct
      901 ggcttatttg accggtacat ggagctcacc caaagccgga accatgacca cccgctacgg
      961 gcgcggtccg gtgatgttgt tttcgacggg ggttatgctg tttggtttac tgatgacctt
     1021 attcagctcg ctgtggctga tctttgccgg aatgttactc ttctcagcag gattcttcgc
     1081 agcccactca gttgccagca gctggattgg cccccgcgca aaacgcgcta aaggccaggc
     1141 ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg
     1201 tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt
     1261 cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa aaataagccc
     1321 ggactgcggt aactacacgt ccggacttat tgcttgctca aaccaacgtt aatagccatc
     1381 ctaaaataga cgaagcgcca gccaattccc gcagcgcgtc tagcgtcatc aggattataa
     1441 gtacccaaat aaacggattc attttgctgt gtgtcattta ttactgatgc gcagttattc
     1501 taccgctttg taagtagtaa aatagttaac ccgatcaaga ctactattat tggtagctaa
     1561 atttccctta agtcacaata cgttattatc aacgctgtaa tttattcagc gtttgtacat
     1621 atcgttacac gctgaaacca accactcacg gaagtctgcc attcccaggg atatagttat
     1681 ttcaacggcc ccgcagtggg gttaaatgaa aaaacaaatt gagggtatga caatgaaaaa
     1741 agtattagct ctggttgttg ccgctgctat gggtctgtct tctgccgcct ttgctgcaga
     1801 gactgcgacc acacctgctc cgactgcgac gaccaccaaa gcagcgccgg cgaaaacgac
     1861 acatcataaa aaacagcata aagcagcacc tgcccagaaa gcgcaggcgg ctaaaaagca
     1921 tcataaaaat acgaaagctg aacagaaagc ccctgaacaa aaagcgcagg cagcgaagaa
     1981 acacgccggg aaacacagcc atcagcaacc ggcaaaacct gctgcacaac ccgcagcgta
     2041 agttttcaac agtaatgctg gcgcgccccc tcgcgcctga aaattacggt gctaagcagg
     2101 taacgtttag caccgccttt agcggagggt agtatggtgg gccgttatcg ctttgagttc
     2161 attcttatca tccttatttt atgcgcactg attgccgccc gtttttatct ttcctgactg
     2221 tagttatctg attttactcc cactttcatc ccgtcccgtc tatagtattt atgagggttt
     2281 gcttttaata atcataatta cccaccagag tgtgatatgc gtacaaccat tgctgtagtg
     2341 ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag ataaaccaga cgttgccaaa
     2401 tcggcaaacg atgaggtcag caccctgttt tttggtcatg atgatcgtgt gccagtgaat
     2461 gacacgaccc aatcaccgtg ggatgcggtt gggcaactgg aaacggccag cggcaattta
     2521 tgtacggcga cgctgattgc acccaatctg gcattaacgg caggacactg tttattgacg
     2581 ccgcctaagg gtaaagcgga taaggcggta gcgctgcgtt ttgtgtcaaa taaaggtctt
     2641 tggcgctatg acatccacga catagaaggc cgcgttgatc cgacactggg aaagcggtta
     2701 aaagcagatg gggatggttg gattgttcct cccgcagctg caccatggga cttcggcttg
     2761 attgtgctgc gtaatccccc ttctggcatt acaccgttgc cgttatttga gggagataaa
     2821 gccgcgctta ctgccgcatt aaaatcggct ggtcgtaaag tgactcaggc gggatatcct
     2881 gaagatcatc tcgatacgtt atacagtcat caaaactgtg aagtgactgg ctgggcgcaa
     2941 acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg acagcggttc gcctttgatg
     3001 ttgcataccg atgacggctg gcaattaatt ggggtgcaaa gttcggctcc tgccgcgaaa
     3061 gatcgctggc gcgccgataa ccgggccatt tctgttaccg gttttcgcga caagctggat
     3121 caactgtcgc aaaaataatg ttcaaacgct gcccgacagt gcgggcagcg tgttcatcag
     3181 gcaagtttca ccatgatcat tccagccaac agcaagacca ggccaatcca gcctttacga
     3241 tttaaccgtt gaccaaacaa gatccaacct gcggctaacg tggcggcaat accaaacccg
     3301 ccccacaatg cataagcgac agacaagtcg atccctttta cggcctgaga aagcgcacta
     3361 aaggcagcca gcaccgccgc cagggagagc aagccaaata ttttgcgacg aaagccgtca
     3421 gaaaatttca aaaagacgtt agcaacgatt tccagcacga ttgccaatgc cagccaggcg
     3481 gcgtgaaccc attcaaactg cgccatggtt cacttccagt tcaggtttac gcgctttacg
     3541 ggtacctgat ttgatcaaca caatcccggc gaccagggtg gttaacccgg caattttcat
     3601 cagcgataaa ctttcgtcga ataacaaaac gctaaacaag gtaataaata aaataccgat
     3661 accttcccac agcgcataag ctacgcctaa ggcgatcttt ttaacggcga aagagagaaa
     3721 tatatacgac agagaaatca tcaccagcat taaaataaag ccgccatttc cctcactgac
     3781 gctcgcccat ttcattgaca gcgtaccggt aatttctgta gcaatagcca ggcctaataa
     3841 aatccaataa atatacattg tccttctcct gcaagagaat tattttaatt ttcgcttaat
     3901 tcagcgaaac caaagtaaag tggtcaagcc ccgcgcatag ggcaagctta ggcagaagaa
     3961 aggactaaag cgcgttgcgc cagtgctgct cacctacgag caagatagtg gatgaggtac
     4021 gaagatgggt aaatgttgaa aataacgtcc tgaacaaatt gtccataata ttacaattat
     4081 ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc tcggtagttg aacacgcctg
     4141 atttgtatca tagcttaaga attaactcaa aatattttca cttctttacc tgagcggttt
     4201 gattttcgtt atgatgacgg agcgaaaaag acattattat tagcaaagga agaaaaaacg
     4261 gggacaagca tggcaaagcc gatcatcacg ctcaatggcc taaaaatcgt cattatgttg
     4321 ggaatgctgg tcattattct ctg
//
LOCUS       DAZGOB010000235         4324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGOB010000235 DAZGOB010000000
VERSION     DAZGOB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.236"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            186..1268
                     /locus_tag="ACHLIF_004970"
     CDS             186..1268
                     /locus_tag="ACHLIF_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFO9608795.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTQANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGKNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1457..1840)
                     /gene="quuD"
                     /locus_tag="ACHLIF_004971"
     CDS             complement(1457..1840)
                     /gene="quuD"
                     /locus_tag="ACHLIF_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO9608796.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1926..2066)
                     /locus_tag="ACHLIF_004972"
     CDS             complement(1926..2066)
                     /locus_tag="ACHLIF_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO9608797.1"
                     /translation="MMFEFYMAERLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2063..2425)
                     /gene="rusA"
                     /locus_tag="ACHLIF_004973"
     CDS             complement(2063..2425)
                     /gene="rusA"
                     /locus_tag="ACHLIF_004973"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO9608798.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2422..2712)
                     /locus_tag="ACHLIF_004974"
     CDS             complement(2422..2712)
                     /locus_tag="ACHLIF_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO9608799.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2705..2875)
                     /locus_tag="ACHLIF_004975"
     CDS             complement(2705..2875)
                     /locus_tag="ACHLIF_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO9608800.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2875..3330)
                     /gene="ybcN"
                     /locus_tag="ACHLIF_004976"
     CDS             complement(2875..3330)
                     /gene="ybcN"
                     /locus_tag="ACHLIF_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO9608801.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3327..3428)
                     /locus_tag="ACHLIF_004977"
     CDS             complement(3327..3428)
                     /locus_tag="ACHLIF_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608802.1"
                     /translation="MRTYNLNYLLPSQMQKCTCDFLYPAFDLCGGEA"
     gene            complement(3552..3953)
                     /locus_tag="ACHLIF_004978"
     CDS             complement(3552..3953)
                     /locus_tag="ACHLIF_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608803.1"
                     /translation="MGKSTLVIGRELTKDMEFSGQFLLKKLKLQNLTIDAAMWFYYPD
                     LSWRYILVISDFSERGPAEIYRKISEINRNSISKKYKPIPLEAIEAKGDSAFVYKMLK
                     GFARVNDGKVRVSNSMVNGLEIVDCLIYELK"
     gene            complement(3932..>4324)
                     /locus_tag="ACHLIF_004979"
     CDS             complement(3932..>4324)
                     /locus_tag="ACHLIF_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HFO9608804.1"
                     /translation="LSDTRLNEAKCLLDHGFFHGAYYLCGYAVECALKACIAKSFLQH
                     EFPNKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKDWSEQFRYDNN
                     ISKAMAEQLFDAVGDQNSGVLKWVKAHW"
BASE COUNT         1213 a          997 c          887 g         1227 t
ORIGIN      
        1 atttttattc agccaagagc catgaatagg attcgataga aaaagttcag ataaaaatag
       61 agatctactt cacaaatcaa atgagaaacc aaaacttaca tcttgaaata atcacattga
      121 ttagatgaat atttatcgcg cagtgacatc attttttaat aatagttcaa aaaaaagggc
      181 tcacgatgaa aaaattaaca gtggcaattt ctgctgtagc tgcatcagta ctgatggcga
      241 tgtctgctca ggcagctgaa atttataata aagacagtaa caagctggat ctgtacggga
      301 aagttaatgc taagcactac ttctcctcta atgatgcaga tgatggtgat actacttatg
      361 cccgtcttgg cttcaaaggt gaaacccaaa tcaacgatca actgactggt ttcggtcagt
      421 gggaatatga attcaaaggc aaccgcgctg aatctcaagg ttcctccaaa gataaaaccc
      481 gtcttgcctt cgctggcctg aaattcggtg actacggctc catcgattat ggccgtaact
      541 acggtgtagc atacgatatt ggtgcatgga ccgacgttct gccagaattc ggtggcgata
      601 cctggaccca aacagatgta ttcatgactg gtcgcaccac aggtgttgca acttatcgta
      661 acaatgactt ctttggtctg gttgatggtc tgaactttgc tgctcagtat cagggtaaaa
      721 atgaccgtac tgacgtaact caagctaatg gtgatggttt cggtttctcc actacttatg
      781 agtatgaagg tttcggcgta ggtgcaacct atgctaaatc tgatcgcact aataatcagg
      841 ttatctacgg taacaacagc ctgaatgcat ctggtcaaaa tgctgaagta tgggcagctg
      901 gtctgaaata tgatgcgaac aacatctatc tggctactac ctattctgaa acccaaaaca
      961 tgactgtttt tggtaaaaac catattgcca acaaagcaca aaacttcgaa gtagttgcac
     1021 aatatcagtt cgacttcggt ctgcgtccgt ccgttgctta tctgcaatct aaagggaaag
     1081 acttgggtgc ttggggtgat caggacctgg ttgaatatat tgatgtaggt gcaacctatt
     1141 acttcaacaa aaatatgtcc acttttgttg actacaaaat caacctgatt gataagagcg
     1201 atttcacgaa agcatctggc gttgctaccg atgatatcgt tgctgtaggt atggtttacc
     1261 agttctaatt tgattactaa aacatatgtt gtgggaggct ttgcctcccc aacatataag
     1321 tggctccctc aagccacttc ctttagaagc acaaccttgc ttctaactat ataaaccttc
     1381 tgttatatat taccctttat ttttgggggc gtttcaacgc cccattctta ataattttta
     1441 gtaaacaatt ggcatattaa ttagagttat taacaacgat atccatctct aaccggatat
     1501 ctaatgccat taacatccct tcaattatgc cctcagcctt ctgtaacctt ttcccgatat
     1561 aaccatcaga gcagcaatgc ttacctgcca gtgacatgaa tgtcataccg actacataat
     1621 aatctactaa taaatcgtgc aaatcgctgt tgttcttttt cagacgggcc atgcacccgc
     1681 aaatgatcat cgcgtcatcg tcacaacatt gcgggcgaga ttttactttt gaagtaatta
     1741 atcccttaaa accggcggca atggacgacc aggtcacatc ttcatgatta ttagccgccc
     1801 acgctcccca acgctcaaga accatctgaa tatcacgcat caacttactc cacaaaaatc
     1861 agaccagaac gccaattaca agcaaaaatc aacgaaacag tattagttga ttgttatctc
     1921 tgacttcata ctcctgctcc tgtcagggtt ttggcgtaat tcttcagtat tcggtaatcg
     1981 gtcaaaacag aaccggggaa acgatataag cgcagacgcc cccagcggtg gcgaagacgt
     2041 tctgccatat aaaactcaaa catcattcat tccccatttc ggtgatggtc agttccagcc
     2101 tcccaccttt ggtaacaggc atcttcacaa cgcggtaatc aacgacctga gcatcatcca
     2161 gccagaaacc tgctttagtg agtgcgtcaa aagcggcttt ttgcagatta tccaggtcac
     2221 ggcgacggcg atccggcatg tggcactcaa tgcggatttt cacaggcata gccaagccga
     2281 tatccagcat tgcgttttta atgattcggg cgacgttatc gcggtatgcc tgcccctctg
     2341 cgctgacgtg cgtgcgcccg cgattatggc ggtaatagcg attattgctc ggaggccagg
     2401 gtaatgtgat gctgtaggta ttcacgcctt gattaccccc tctttcagcc agataacctg
     2461 cgttctcgcc ataccttcca gcgcgcattc ttttgcgtac tcagcatcga caaaatgcgt
     2521 gcggcggtcg atttcgtcgt gacaggcaga acaggcaatg gtggcaataa ggtctggcgg
     2581 tttaataccg gtgccacaca gtccagccag ccggatatgt gccagtacag acgtttcagg
     2641 gttgccattg catacgccag ggattcttac ctggcattcg cgaccacgcg ctgcttttct
     2701 caaatcagcc atgattcctc cttgctgcca gtcgcaacca ttttttatca accaggctgg
     2761 cggtatatcc gagcagtgtt ggtatttcgg atggttttag ctcaggctta cgcttacgac
     2821 gatttggtac tctgtagatg tgtccgttca tgacacgaat aagcggtgta gccattacgc
     2881 ctcctgcttg tcgcgcagca gctggaactc gcagctctgc ggaatagtca ggtggcagcc
     2941 aatattcatc gcccaggctt caaccttaca caggaagaca tacatctctc cggtatcaag
     3001 atcggaggta tggcgtaacg actggatagt agtgatttcg ccggttacga catcaaccag
     3061 gtccttggtt tcataaccga ggtatgtgtg tttgagagca tcttttaccc atgctgcggt
     3121 agcgaacgat ttccccctgc ggataaggta ttcactgatt tcgctgtacc acatgtggct
     3181 aagtgcattc tgggaaagac tgcgtctctc gcgccacggt ttaagcacca tgcgaaagca
     3241 cttgccgtct tccaaataag gctggagctg ctgaccgata gcggtgaagt taccgcgatg
     3301 cagtttgatg ccgtcttgtg ggaggttcac gcttcacctc cgcagaggtc aaacgctgga
     3361 taaagaaaat cgcaggtgca tttctgcatc tgtgacggga gaagatagtt tagattgtat
     3421 gtgcgcataa acgtccccgt ttagcgcaga agtcaccgga ggagttcaaa ctccggtgac
     3481 ttaattatga caagttgatt attgaaaatc aaaagtcatt taaacaataa tgatacccag
     3541 caagagattt cttattttaa ctcatagatc agacagtcaa cgatttctaa accatttacc
     3601 atagaattag aaacgcgaac tttaccatcg ttgactctag caaatccttt taacatttta
     3661 taaacaaaag ctgaatcccc cttagcctca attgcttcta atggtatcgg cttatacttt
     3721 tttgatatgc tatttctatt tatctcactg atttttctat atatttctgc cggcccacgt
     3781 tctgagaagt cactgataac taaaatatat ctccaagata gatctgggta ataaaaccac
     3841 attgcagcat caatagttaa attctgtaac ttgagttttt ttaacaaaaa ttgacctgag
     3901 aattccatat cttttgtcaa ctctctgccg attaccagtg tgcttttacc catttcaaaa
     3961 ctccagaatt ttggtcacct acagcatcaa acaattgttc ggccatagct ttacttatgt
     4021 tattgtcgta tctaaattgc tcgctccagt ctttaacgac cgaccagtta atctccaacg
     4081 aaacatcatt ttttgcgtca gcaatcaaaa tttgatgtaa gttggcaatt ttgagaagtt
     4141 gcgacaaatc atgagtatat gaatcattta cgactttttt gtttggaaac tcatgttgta
     4201 aaaatgactt ggcaatacag gccttcaatg cacattcaac tgcataccca caaagataat
     4261 atgcaccatg aaaaaagcca tgatcaagca agcatttggc ctcattaagc ctagtgtcag
     4321 ataa
//
LOCUS       DAZGOB010000236         4317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGOB010000236 DAZGOB010000000
VERSION     DAZGOB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.237"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            381..989
                     /locus_tag="ACHLIF_004980"
     CDS             381..989
                     /locus_tag="ACHLIF_004980"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO9608805.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1087..2478
                     /gene="selA"
                     /locus_tag="ACHLIF_004981"
     CDS             1087..2478
                     /gene="selA"
                     /locus_tag="ACHLIF_004981"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO9608806.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2475..>4317
                     /gene="selB"
                     /locus_tag="ACHLIF_004982"
     CDS             2475..>4317
                     /gene="selB"
                     /locus_tag="ACHLIF_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO9608807.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          965 a         1105 c         1266 g          981 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggcaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgccgccgg ttttccgctc atctgctcat ccttgtttta tcgggttatt
      181 tgtctgcttc caggcaaaac cctacagatt tataagaaag ccttaagtga gatatccatc
      241 agatagaaac aattaggaaa atatttatat caccatgata aatatgtgat ttatttggaa
      301 gtaacaaacc atcaacattt tctgcccttt ttcccatcac caggccatac tgatattttt
      361 cctcgcacaa aggactcggc atgaaactcg tcggtagcta caccagcccg tttgtacgca
      421 aactttctat tctgttgtta gaaaagggca taactttcga atttattaat gaactgccct
      481 ataacgcgga caacggcgtg gcgcaattta acccgttagg aaaagtgccg gtgctggtga
      541 ccgaagaggg cgaatgttgg tttgattcgc cgatcatcgc tgaatatatt gaattaatga
      601 atgtcgctcc ggcgatgttg ccgcgcgatc cgctggagtc gttgcgggtg cgcaaaattg
      661 aggcactggc ggatggcatt atggatgccg ggctggtatc ggtgcgtgaa caggcgcgtc
      721 cagcggcgca gcagtctgaa gatgaattgt tacgccagcg ggagaaaatc aaccgcagtc
      781 tggatgtgct ggaaggatat ctggtcgatg gcacactcaa aaccgatacg gtcaatctgg
      841 cgactatcgc cattgcctgt gccgtcggat atctcaattt ccgccgcgtt gcgccaggct
      901 ggtgtgtcga tcgcccacac ttagtcaaac tggtcgaaaa cctgtttagc cgcgaaagtt
      961 ttgctcgcac cgaaccgcca aaggcttgat gcgcgatatg tcctcctgac ccatctcacg
     1021 ttacaatccg tggttatgtt aaacgccctt ctccgtgtga gagggccttg atcagccagg
     1081 tttcctatga caaccgaaac gcgttccctc tatagtcaac ttccggctat tgatcgctta
     1141 ttgcgcgata gctccttcct ttctttgcgt gatacttatg gtcacacccg cgtggtggaa
     1201 ttgttgcgtc agatgctcga cgaagcgcga gaagtgattc gtggcagcca gacgctgcct
     1261 gcgtggtgtg aaaactgggc gcaagaagtc gatgcccggt tgacgaaaga agcgcagagc
     1321 gcgctgcgtc cggtgatcaa cctgacggga accgtgctgc ataccaacct tgggcgagct
     1381 ttacaggcgg aagccgcggt ggaagccgtt gcgcaggcta tgcgttcgcc agtgaccctc
     1441 gagtacgatc tggacgacgc tggacgcgga catcgcgatc gtgcgctggc gcagttgctg
     1501 tgccgcatta cgggggcgga agatgcctgt atcgttaata acaatgcggc ggcggtgtta
     1561 ttgatgttgg cggccactgc cagcgggaaa gaggtggtgg tttctcgcgg cgaactggtg
     1621 gagattggcg gcgcgtttcg tattcccgat gttatgcgtc aggcaggctg caccctacac
     1681 gaagtaggga ccaccaaccg cacgcacgcg aatgattatt gtcaggcggt gaatgaaaat
     1741 accgcactgt tgatgaaagt acataccagt aactacagca ttcaggggtt caccaaagcg
     1801 atagatgaag cggaactggt ggcgctcggc aaagagctgg atgttcccgt agtgactgat
     1861 ttaggcagtg gctcgctggt cgatcttagc cagtacggtt tgccgaaaga gccaatgccg
     1921 caggagttga ttgcggcggg cgtcagtctg gtgagtttct ccggcgacaa gttgttaggc
     1981 gggccgcagg caggaattat tgttggtaaa aaagagatga tcgcccgcct gcaaagccac
     2041 ccgctgaagc gtgcattacg cgcggataaa atgaccctcg cggcgctgga agccacgttg
     2101 cgtctttatt tacaccctga agctctgagt gaaaaattac cgaccctgcg cctgcttacc
     2161 cgcagcgcag aggtcattca aatccaggca caacgtttac aggcccccct tgccgcacat
     2221 tacggcgcgg agtttgcggt acaggttatg ccatgtcttt cgcagattgg cagtggttcg
     2281 ctgccggttg atcgcctgcc gagcgcggca ttaacgttta caccccatga tggacgcggt
     2341 agccaccttg agtcattagc cgcccgctgg cgtgaattgc cagtgccggt gattggtcgt
     2401 atttatgacg gacgattgtg gctggattta cgctgccttg aagatgagca acggtttttg
     2461 gagatgttgt tgaaatgatt attgcgactg ccggacacgt tgaccacggc aaaacaacct
     2521 tattgcaggc gattactggc gtaaatgctg accgtctgcc ggaagaaaaa aagcgcggca
     2581 tgaccatcga tctcggctat gcctactggc cgcagccgga tggtcgcgtg cctggtttta
     2641 tcgacgttcc cggtcatgaa aaatttcttt ccaacatgct ggcgggcgtt ggtggtatcg
     2701 atcacgcgct gttggtggtg gcgtgcgatg acggtgtgat ggcacagacc cgtgagcatc
     2761 tggcgatttt gcagctgacc ggtaacccga tgctgacagt ggtgctgacc aaagccgatc
     2821 gcgtggacga agcgcgtgtt gatgaggttg aacgtcaggt aaaggaggtg ctgcgggaat
     2881 acggttttgc tgaggcaaaa ctgtttatca ccgcagcaac cgaaggtcga ggaattgatg
     2941 ccctgcgcga gcatctgctt cagttaccag aacgtgaaca cgccagccaa catagtttcc
     3001 gcctcgcgat cgaccgcgca tttaccgtaa aaggtgccgg gctggtcgtc accggtacgg
     3061 cgttaagcgg ggaagtgaag gtaggcgatt cactatggct aactggtgta aataaaccga
     3121 tgcgtgtacg tgcgctgcat gcacaaaacc agccaacaga aaccgcccat gccgggcagc
     3181 gtatcgcgct taacatcgcg ggtgatgcgg aaaaagagca gattaaccgt ggcgactggc
     3241 tgcttgccga tgcgccgcca gagccgttca cacgggtgat tgtcgagctt caaacccata
     3301 cgccgctgac ccagtggcag ccgctgcata ttcaccacgc cgccagccac gttacgggac
     3361 gcgtttcgct gctggaagat aaccttgccg agctggtgtt cgacacgccg ctgtggctgg
     3421 cggataacga tcgcttagtg ctgcgcgata tctcggcccg caacacgctg gcgggggcgc
     3481 gtgtggtgat gcttaacccg ccgcgtcgcg gtaaacgtaa gccggaatat ctgcaatggc
     3541 tggcgtctct tgcacgggcg cagagcgatg ccgatgcgtt atctgttcat ctggaacgcg
     3601 gcgcggttaa ccttgcggat ttcgcctggg cgcgccagct caacggcgaa gggatgcgcg
     3661 aattgctgca acagcctggt tatattcagg ctggttatag cttgttgaat gcgccggttg
     3721 ccgcccgctg gcagcggaaa attctcgaca cattagcgac ttatcatgag caacatcgcg
     3781 atgaacctgg tcctgggcgc gaacgtctgc gacgtatggc gttgccaatg gaagatgaag
     3841 cgctggtact gttgctgatt gaaaagatgc gcgaaagcgg cgacatcctc agtcatcacg
     3901 gttggctgca tctgccggat cacaaagcgg gcttcagcga agagcagcag gccatctggc
     3961 aaaaagcaga gccgctgttt ggtgacgagc cgtggtgggt gcgtgatctg gcaaaagaga
     4021 cgggtaccga cgagcaggca atgcgcctga cgctacgcca ggcggcgcag caaggaataa
     4081 ttaccgcgat cgtcaaagat cgttattacc gtaacgatcg gattgtcgaa tttgccaata
     4141 tgatccgcga tctcgatcag gagtgtggtt caacctgcgc ggcggatttc cgcgatcgct
     4201 taggcgtagg ccgaaagctg gcaattcaga ttctggaata ttttgaccgc attggcttta
     4261 cgcgtcgtcg tggaaatgat catttattgc gcgacgcatt attatttccg gaaaaat
//
LOCUS       DAZGOB010000237         4312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGOB010000237 DAZGOB010000000
VERSION     DAZGOB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.238"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..365)
                     /locus_tag="ACHLIF_004983"
                     /pseudo
     CDS             complement(1..365)
                     /locus_tag="ACHLIF_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(325..444)
                     /locus_tag="ACHLIF_004984"
                     /pseudo
     CDS             complement(325..444)
                     /locus_tag="ACHLIF_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(457..2067)
                     /locus_tag="ACHLIF_004985"
     CDS             complement(457..2067)
                     /locus_tag="ACHLIF_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO9608808.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSNGSMTLMLKEMSNLINDEGIK"
     gene            complement(2742..3731)
                     /locus_tag="ACHLIF_004986"
     CDS             complement(2742..3731)
                     /locus_tag="ACHLIF_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608809.1"
                     /translation="MTMTKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALALAK
                     AVKTMLNGVLKSAQDNIPFPIEILPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDAKSSTGFTQGTLQQDALNICDNVVRTINSRMV
                     DVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTEPSFGSEVADYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGEDRGVFMMAKRFTALSFGCFRHDGDHITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(3806..>4312)
                     /locus_tag="ACHLIF_004987"
     CDS             complement(3806..>4312)
                     /locus_tag="ACHLIF_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608810.1"
                     /translation="SSKASALFNNQDWKLSVTDINLYENTVSLDGQFYPLSLAIKTLI
                     PGYLSGLPSTSRESMELLEALAEAGVTIGNFFSNDLMTAYGRRQQNKRAEAERIAKEQ
                     RIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLES
                     GGNWMDNL"
BASE COUNT         1117 a         1001 c          941 g         1253 t
ORIGIN      
        1 caaatacttt acctacgtgc aaacgattat attttagttc aggatgcttt agatttaacg
       61 gggaactgga taaactgagc cattccccta ttaacctttc cgtttctggc attaaagcca
      121 tcccagcgag ctcagatgaa attaagggaa atatttctcc aagcaatacc catccagacc
      181 agctgccact ctcttctgct atcggtaacc atttagcact aaattcgggc attaataatg
      241 caacatcttc gccattttct ttccaatttt gaaaagtggt ttcaaacgta tgagtgtttc
      301 gatttacttt cctcagaccg aacattaact ttcatccttc tgatcgtttt cgaatttgct
      361 ttcattttct tgctcctcat aaggctgatg tcgttgtcct tgtcgttcat cttgctcagt
      421 caatacccaa cgttgtgctg taactcatta tgaaaactat ttaattccct catcattgat
      481 aaggttggac atctctttca acatgagagt cattgaacca ttcgaccaat ttagcggaat
      541 gatagggctt ggcgcttcat gcaaagttcc cgacttgtgg atatagttat agctttcggg
      601 aagtgccatt tcaggtacac tcctgccatt tgcaccaatc atattctcgc ctgttatttg
      661 ggccaatgat ctattcataa attgcactga gtcattcaaa tactcttcct ttcttgattc
      721 tttataaaca attgctgctg attttgcata ccatgagtca aaaaaccatt ctgcttctgt
      781 tgatggaatg aagctctttt ctcttttcgc atgtgaattt tgatcggtat cggttttaat
      841 atcattgaac caaaaatttg ccgactgata attatctttc tcgtatcttt ttataccata
      901 cggacctgct agttttttaa ctatttttag aacctgctct tttcttctgg tgtttatctt
      961 tgcaagattt gcaggataaa taacattaag taaagccgca tcggcttctc tgtaatgttt
     1021 atctttttcc agataacctg gggattctcc ccctaaatca agctgtaacg ttattctttc
     1081 ataaccttta tctatgaggt ggttcaacct cgttgttgaa agaggttcat ccagttcatt
     1141 ggctttagct tcgcgtagta aatcagagac aaaaacagag tctttcttcg agagtaagtt
     1201 cgacaaacgt tccagtccag aggtcaccag tgcaacggaa gatgtgttta atcgggcatc
     1261 ttcttcccat gctccggaat cttccataac atagaaattg gctttatcca gataagcaat
     1321 cagcagcgca accgatttta atcggtcacc tttttgccaa tcttcagcgt taattgtatc
     1381 agtgtcgatt gcttgtataa ggagatcaag ataaagacct aacgcatcat tttgtttatg
     1441 gttccacagc tgcggcttgc cttcttcctg cacatctgcc atcacggggg agttgctgtc
     1501 aaatcggata tgcacaacat tcatttgccc gggaactcca tccagtcgtt tggggttgct
     1561 aatgacgtcc tgcatccgct taatctggtc gggcgtagac atataatccc acagggtgag
     1621 cagaaccttt tttgcggcta cactatttcc ctgatctgat actaatgcca tatagcccca
     1681 taaactatcc ctcagccaga cagcatcata gtgggtttct gccgctgttt tgtcatcttc
     1741 gaaagtagaa gcaagaataa acgttcccca gggtttctcc gtcaacgtta actttcgtgt
     1801 gttttctgtt aatgttagca atccttgaat ttcgtctttg gtatagtggc tctttatttc
     1861 attttgtaat ttggctgagt gaatcaaaag acgataatta ggatacttat ctggatttaa
     1921 cgataaataa cttttatctg ttgctgcccc cgtggaatta agaatgttaa atacagattc
     1981 actcgtcact tctgcccctg tcgcctggca tgtgccgcac gaaaatatac cagcgagcag
     2041 aagagttaat gcagatttcc ctttcataaa atattccatc aatcgtttgt taaaagcgta
     2101 gataaccact tatttaacaa cttgaaaata aggtgtttta cctgggttgt tacaaaagga
     2161 ttgcattgcg taaacgcttt ttatttacaa caaaatgggg aagtattacg gcgattgcga
     2221 aaagtctgaa gcgattgaat taagataata tagaaaatca gatacagcaa aaatggcaga
     2281 ttgatcaggt gaagataaat tggagcgggc gaagggagcc tacgacaaat cgaagacaac
     2341 cagctaacct cttgtttctt aaagactgat gaatccagtt ggttgtagtg atagtcacac
     2401 atttagcgat agacggtcaa gacttgaagc acgtaatatt ccctctaaag gaatagaact
     2461 cacggtctga ctacccggcc cttttggtct gactcagggt cgggctatgg ggacttttgg
     2521 ctcgctcttt atattagatg aggtctcaca tttttcagtc aaaactcatc gtctaatagc
     2581 ccatagccag accaccagtc ccagctttag caggaagacc acgagataga ctttgtgtag
     2641 gtccatttgg tcctcctccc ttctgtattc acgaactaga ttactagtac aaaataaagg
     2701 ccagacatcc agttcggaag tctgaccaat agtcgttatt gttagttagc ttctttagtg
     2761 aagctcaggc gtttgactac aggcaggaca gcagtagccg agccagtccc catcttgatg
     2821 gtcgtagcag cttcagctac aacaaacacc ttgccgagaa tctcaaggtc gtgagactgg
     2881 gagtcaccat cacgtgaaag gaccacagta atgtggtccc catcgtgacg gaagcaaccg
     2941 aagctcagag ccgtaaaacg cttagccatc atgaagacgc cacggtcttc accgttgtaa
     3001 taggcgatgt cagtccccaa catatcgacg atagcgtctt caccaccaaa gccctgctta
     3061 gcagcaagac gtgacagtgc cttgaggtgt gactcatggg cgatgatgcc gtaatcagca
     3121 acctcagaac cgaaagaagg ctcggtgttc tcgtacagcg cgtccatgat agtgtctgcc
     3181 ttagctgtca ggctaccagt caggaccgga agctctacag tcttcaacag cttggaggat
     3241 tccagtacat cgaccatgcg actattgatg gtacggacca cgttatcgca gatgttcaga
     3301 gcgtcctgct ggagcgtccc ctgagtgaaa ccagtggaag acttagcgtc ccatagcttg
     3361 gtctggtggg acacgaggat agcttgctca atcggctcaa cggtgatttc agtaccagac
     3421 ggagcggtca cgacttcagg tcgcttacca ccacggacag gaacgaccac agagcggtca
     3481 gccagagtgt gagcaatgat tggggtcggt aagctattgc gagttggcag aatctcaatc
     3541 gggaatggaa tgttatcctg agcggacttc agcacaccat tcagcatggt cttgacagct
     3601 ttagccagtg ccagagcata gacctgtggg tcagcattgg ttggcagacc ttcgttaacc
     3661 tgaatgttgt ctgtgtcggt gatacgtact ggctcaatgc cgaactgata gctgtcttta
     3721 gtcatagtca taataatcaa tctcctaaat tagtttagat acgtgggtcc cgacatcagg
     3781 tcaggaccac ttgtgtgcgt ctgtcttaca ggttgtccat ccagttaccg cctgactcaa
     3841 ggtcagcgac gatagcggca cgggagcgac cagcggaatg ggtagcacta cgaaggttct
     3901 caccgtaggt ccggcgttca gctttgacct gttcacggcg ctgtagctct ttctgccact
     3961 ctgcatcagt catgttctct tcacgcatgc gctcggcctg aatgcgctgc tctttagcga
     4021 tacgctcagc ctcagctcgc ttgttctgct ggcggcgacc ataggcggtc atcaggtcat
     4081 tactgaagaa gttaccaatg gtcacgccag cttcagccag ggcttccaat aactccatag
     4141 actcgcggct ggtagacggc aggcctgaca ggtagcccgg aatcagggtc ttgatagcga
     4201 gggacagcgg atagaactga ccgtccagac tcacagtatt ctcgtagaga ttgatgtcgg
     4261 tgactgacag tttccagtct tggttattga ataacgcact agctttgcta ga
//
LOCUS       DAZGOB010000238         4256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGOB010000238 DAZGOB010000000
VERSION     DAZGOB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.239"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..168
                     /locus_tag="ACHLIF_004988"
     CDS             <1..168
                     /locus_tag="ACHLIF_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608811.1"
                     /translation="WARKFSEQIFKITGDENAAKNELEPWTPEGNAPNYCWWEVDPVD
                     VANEAMSYHND"
     gene            202..384
                     /locus_tag="ACHLIF_004989"
     CDS             202..384
                     /locus_tag="ACHLIF_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608812.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            377..553
                     /locus_tag="ACHLIF_004990"
     CDS             377..553
                     /locus_tag="ACHLIF_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608813.1"
                     /translation="MADFGSTKYSVSFEEWHELLMEYAELRGGSAADAEAWRDDYEAG
                     KNPVEAYCDEWGDE"
     gene            550..1047
                     /locus_tag="ACHLIF_004991"
                     /pseudo
     CDS             550..1047
                     /locus_tag="ACHLIF_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            1273..1491
                     /locus_tag="ACHLIF_004992"
     CDS             1273..1491
                     /locus_tag="ACHLIF_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO9608814.1"
                     /translation="MTTLFRKEYPRKSRATEFLFLIIFIVLMIPIPPLLLVWFIVKII
                     EPVIELYNDVVWASFSTLHNKINPYKES"
     gene            1493..1858
                     /locus_tag="ACHLIF_004993"
     CDS             1493..1858
                     /locus_tag="ACHLIF_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO9608815.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            1855..2199
                     /locus_tag="ACHLIF_004994"
     CDS             1855..2199
                     /locus_tag="ACHLIF_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608816.1"
                     /translation="MTTFTREQLIAHAEETIETQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            complement(2404..2703)
                     /locus_tag="ACHLIF_004995"
     CDS             complement(2404..2703)
                     /locus_tag="ACHLIF_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9608817.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDSRKVFIVGIYHGGQSYETDFL"
     gene            complement(2709..2966)
                     /locus_tag="ACHLIF_004996"
     CDS             complement(2709..2966)
                     /locus_tag="ACHLIF_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO9608818.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            3102..3374
                     /locus_tag="ACHLIF_004997"
     CDS             3102..3374
                     /locus_tag="ACHLIF_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608819.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            3376..>4256
                     /locus_tag="ACHLIF_004998"
     CDS             3376..>4256
                     /locus_tag="ACHLIF_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9608820.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQKPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTHWVKTQPCA
                     CCGKPADDPHHLIGHGQG"
BASE COUNT         1199 a          918 c         1134 g         1005 t
ORIGIN      
        1 tgggcaagaa aattctctga gcagattttt aaaattactg gcgatgagaa tgcggcaaaa
       61 aatgaattag aaccgtggac acctgaagga aacgcaccaa attattgctg gtgggaggtt
      121 gatccggttg atgttgcaaa tgaagccatg agttaccaca acgattaatg tcaggaggcc
      181 gcccgaaagg gcggtagtta aatgcgaaag tttaaaataa ttattgaaac gggaatagcc
      241 ggtggagatt tcgaggatga attcgaagtg gatgatgatg cgacgcctga tgaaatacat
      301 gacgaagcaa aagatatttt ctttaactac tgcaattatt catatcacga aataaaagac
      361 gaagaggaag aacaaaatgg ctgattttgg ttcaactaaa tacagcgtca gttttgaaga
      421 atggcatgaa ctgttaatgg aatatgcaga gttacgtggt ggcagtgctg ctgatgctga
      481 agcatggcgt gatgattatg aagcaggaaa aaatccggtc gaagcatatt gtgatgagtg
      541 gggcgatgaa tgagcgagat tgactatcag gcgctgcgtg aagcagcaga gaaagcaact
      601 aaaggatgct acatcgtagg gcatacatcg ggcaatcagc atgggaatat aacaggagtt
      661 tttgtttgtc aaaaatggaa aggagagccc ggtggcgtaa ttgcagaatg tcatgttaac
      721 tgcctggttg aaacagatgt tcaggcttat gcaaacgctg aatttattgc tgcttttaat
      781 ccaaatgttg cgctggcact actggatgaa cgggaagccc aaaacaaacg aattgcagag
      841 ctgaaagcta atcttgtggc gctggcggcg gagaatgcgg taataaagtc tgcaattcca
      901 gaaccacggg atattgagga cgacaatgac aatatggatg acgtatctct tgctgaagat
      961 ttcgggttca atcatgcaat agaacggatg aggagacgga ttcctgaaac tccggccacc
     1021 gacgctttcc tggctgaagt acgggcgggg ttgtttaacg agctttgcgc ggcgtttgtc
     1081 aggtacgaaa aagttgcagg actggatgat agcgatacag taacgcttaa ggaagcgaca
     1141 gaagcattgc tggattgtgc ggaacagctt atcgcgcctg aataattaaa tttagttaaa
     1201 aatttaatcc ttaaccggag ggatttctgc accctcaaaa catcaggagg ccgcctgaaa
     1261 gggcggtaag aaatgactac attattcaga aaagaatatc cgagaaaaag tagagcgaca
     1321 gaatttttgt ttctcattat atttatcgtg ttgatgatac cgataccccc gctattactg
     1381 gtctggttta tcgtgaaaat aattgagcca gttattgaat tgtataacga cgtggtgtgg
     1441 gcgtcgttca gcacactgca caataaaatt aatccgtata aggaaagctg atatggcact
     1501 gacgaaaaaa caacgtgcag agctgcgcat gaagttcggc ggtcgctgtg cttattgcgg
     1561 ctgcgaactt ggcgaaaagt ggcatgcaga ccatgtaaaa ccggtcattc gttttgatgg
     1621 aaatatgctt caccaggaac gtgacgatat atccaacatg gttccggcat gccacccatg
     1681 caatctgcac aagcattgca gtagtctgga agattatcgg cgaattatca gtgatggtcg
     1741 tcgtgaattc cttgcgtccg ggaaaggcaa agcgctggtt cgtatgggat tggttgaaat
     1801 gaaatctgac ccagttgtgt tctggtttga aaaatatcaa gaaggggcta cggcatgacg
     1861 acttttacca gagagcagtt aatagctcac gcagaggaga ctattgaaac acagagactg
     1921 tgcataccgg gcacaatcga ccatgacatc atccgcacat ataagatgga tattgctgtt
     1981 ctggaaatcg cactggtatc gctggcagca gagccagccg gtaaattgca tgaatacaaa
     2041 ccagtgggat atcagcgtct ggtcgatgag ttaaccatgc tggtaaagca gttaacctgg
     2101 caactgagga aagcgaagcc agactgcaaa ttaccggata aggcgatgag ttatctggag
     2161 cggaacggac tgataagcgt ggaggatatt ttacgatgac ctggcctgaa gcattcacaa
     2221 cggtaggaat tgcgatggcg gtggcgctgg tggtgtattc gatttgccgc tggggataaa
     2281 tcgccgaaaa aagatcccga cataaacatg agccgggatc tttgatttat atagcctacg
     2341 aatccgccag taagagaggg ggcggacggt taattctaac accggaatga tgtgggtaaa
     2401 agtttataag aaatcggttt cataactttg cccaccatga tagataccga caataaagac
     2461 ttttctgcta tcaacggcaa aagcaataat cgttctgtgg cggaaatgag ttacccgcat
     2521 cccctggcga atatcatcgc gtttattgcc ccgatgcggg aatgtagaaa acccatcaag
     2581 ataatcaaga agcgcattgg cataattgtc agcaatgacg ttccctgctt tctccgttat
     2641 atacctgtgc aggttgatta tttgttgttc ggcctcagga gtaatgatga cttcatatgt
     2701 catgcagatt acttcccgga tcgaatcgcg gcgcgaacct gtgaaatgga gcgtccgttg
     2761 tttggatttt cgcggataga atcaagagag ggggcggctg aatgcgttaa ccacgcttcg
     2821 attgctttat cgcgctcatt cagtgcgcga agcccttcac gaatgacctc gctttctgaa
     2881 gcataggcac cggaagccac acgggcgcgc accatgtcag ccatttcgtt agttaatgta
     2941 atgctgaatt gttgggttgt acgcatggta aacctcacgg agtaggatag aacaccattc
     3001 gatgatagca cgttgcctgt tgacgacaac agaaatcaga gacaatattg ccgcacgcca
     3061 gcttgaacaa ctggcacctg ctgcgccagc agagaaaacc gatggcgcac aataccaaac
     3121 atcacaattc tgatatcgcc cctgccagca ggcaagggcg gtgttctcac acattcaaat
     3181 atgactggta tcagcacgat ccctgcactg aagaacaggc cgaatggctg attcataact
     3241 accgcagacg tgggtatgag tttaagaaag cccttagcct cgactaccgt cactggataa
     3301 tctacgtcag gctcctttat tccgaacgcc cgccgcgtcc gtcccgcaca ttccagcaac
     3361 gcatctggag gtaacgtgcg ggtattactt cgacctgttc tggtaccgga actcgggctg
     3421 gtgatcgtta agccgggtcg tgaatccatg ccggtattcc acaatacccg ggtactggtg
     3481 gagccggaac cgaaaagcat gcgtaatctg ccgtccgggg ttgttcctgc cgttcgccag
     3541 ccgctagtgg aagacaaaac attgctgccg tttttcagta acgcacgggt gattcgtgct
     3601 gctggtggtg ctggtgcatt gtctgactgg ctgttgcgcc atattaaatc ctgccagtgg
     3661 ccacacggcg attatcatca cagcgaaacc gtcattcacc gttatggtac cggcgcaatg
     3721 gtgttgtgct ggcactgcga caaccagctg cgcgaccaga catccgaatc actcgggcag
     3781 cttgctcaac aaaatctgac agcctggatg attgacgtca tacgtcacgc aataagtggt
     3841 gcacaggaac gggaattatc tctggctgaa ttatcctggt gggcggtccg caatcaggtg
     3901 gcggacgcgc taccggaagc ggtattacgt cgttcgctgg ggttgcgtgc ggaaaaaatc
     3961 cgctcaatgt accgtgaaag cgacatcgta ccgggagagc agaccgccac cagcatactg
     4021 aagcagcgca caaaaaatct tgcgccgctg cctcacgctc accagcaaaa accgccacag
     4081 gaaaagacgg tggtcagcat tgccgttgat cctgagtctc cggaatcttt catgaaacga
     4141 cctaaacgtc gccgctgggt taacgagaaa tacacgcact gggtgaagac acagccgtgt
     4201 gcgtgttgtg gtaagcctgc tgacgatccg catcacctga ttggtcatgg tcaggg
//
LOCUS       DAZGOB010000239         4215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGOB010000239 DAZGOB010000000
VERSION     DAZGOB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.240"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            419..982
                     /locus_tag="ACHLIF_004999"
     CDS             419..982
                     /locus_tag="ACHLIF_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9608821.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            1137..3497
                     /locus_tag="ACHLIF_005000"
     CDS             1137..3497
                     /locus_tag="ACHLIF_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO9608822.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(3659..3835)
                     /locus_tag="ACHLIF_005001"
     CDS             complement(3659..3835)
                     /locus_tag="ACHLIF_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608823.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(3973..4215)
                     /locus_tag="ACHLIF_005002"
                     /pseudo
     CDS             complement(3973..4215)
                     /locus_tag="ACHLIF_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1076 a          866 c         1065 g         1208 t
ORIGIN      
        1 ggtctgtata ccatcatttt tatggtgaag ttaaatatct gtagaggcct caagctgggt
       61 cgctgttttt acttaatggt acagaagtat taatagcggt gttgcctgtg gctacccgat
      121 actggctttt gtgaatgtca gattacttcc tgtaagtgat tgaatggtac ttccttaaac
      181 tcaattctat gaggtgtgtt tttaccattg taattcgtac tggtcctcag tttaagtgag
      241 tcttactgta ctctgaaata ttacctcttt ggagtaatga ggtaatacga aacagtttta
      301 tcaggttgtc tgtgctgctg gttacctcgt ttattaatgc cgcggtacgg cgtttctgta
      361 ctctcagtat ttatgtaatt ccaccttcct gtaactttta taaagtaagg actataaaat
      421 gtataatgct aatcccaatt acgaaatgga cttcatgatt ctgaaagacg tgaatgaaca
      481 catggaaggt ctgtttcaac gtttttctaa actgttacct ttcagaattg attttgccta
      541 caggaaagac acgcccagtt ttggtcattc ctgtaggcac agtatgtgta ttgaaatgca
      601 ccgcttactt agtgaaaccc aaacgatgct ggcgggatat tactgggtaa tggaatatac
      661 gtcaaacaag ggacttcata ttcattttat cggctacctg gatgggcaac gtcacaacaa
      721 atcttatcgg atatccaggc aactgggaga tatctggagg cggatcacgg aaggggaggg
      781 ttattttcac ctgtgcaggg ctaaagacaa atacccggtg cgtatcgatc acgtaattca
      841 ctatagtgat aaatctgcgg tagacgactt acgttacgcc cttagctatc tggccaaaca
      901 ggatcagaaa gaacacggta ttattctgag gcgtagcagg cttcccgaaa aaagtaaccg
      961 gggccgacca cgacataact gaattacagc tgtattaggt attagaatta agtttctgta
     1021 agttgttggg aggtgcttga gttaataggc agaagagggc ggggcgcttt gtcatcagtg
     1081 taggggaccc gaatttctgc cgtggtgcct cagtggatcg gatacaggta acgaatatgg
     1141 ttcataaatc tgacagtgat gaattagctg cattgagggc agaaaatgcc cgtctggtct
     1201 cattacttga agctcatggg attgaatggc gacgtaaacc gcagactcct gtgcagcgcg
     1261 tttctgtatt atccaccgac gagaaggttg cattatttcg tcggttgttt cgtgggcgtg
     1321 atgatgtatg ggcacttaga tgggaaagta aaaccagcgg caaatcaggg tactctcctg
     1381 cctgcgctaa cgaatggcag gcgagaatat gcggtaaacc ccggataaaa tgtggggact
     1441 gcgctcaccg tcagttgatt cctgtatccg atctcgtcat ctaccaccat ctggccggta
     1501 cccatactgt cgggatgtat ccgttgctgg aagatgattc ctgttatttt ctggcagttg
     1561 attttgatga agctgaatgg cagaaggatg catccgcatt catgcgatcc tgtgatgagc
     1621 tgggtgtacc tgctgcgctg gaaatatccc gttcacgtca gggggcgcat gtctggatat
     1681 tttttgcctc acgagtttcg gcccgcgaag ctcgccgtct ggggactgct attattagct
     1741 atacgtgtag tcggacccga cagctgcgat tggggtctta tgaccgatta tttcctaatc
     1801 aggatactat gccaaaaggg ggatttggta atctcatagc gttacctctg caaaaaagac
     1861 cgcgtgaatt ggggggaagc gtttttgttg atatgaatct ccagccttat cctgatcagt
     1921 gggcttttct tgtatcggtg accccgatga atgtgcagga tattgaaccg acgatattac
     1981 gggctacagg gagtatccat cctctggatg tgaattttat caacgaagaa gacctgggta
     2041 cgccgtggga agagaaaaaa tcatcaggaa acagactgaa tatttctatt gcagaaccgc
     2101 tgaaaatcac gctggcaaac cagatctatt tcgaaaaagc gcaattacct caggtgctga
     2161 ttaaccgact tattcggctg gcagcatttc cgaaccctga gttttataag gctcaggcaa
     2221 tgcgtatgtc agtctggaat aagccccgtg ttataggttg tgcggagaat tacccgcaac
     2281 acattgcgtt gccccgggga tgtctggaca gcgtattatc tttccttagg gacaacaata
     2341 ttgctgcaga attaatcgat aaacgatttg ccgggacgga atgtaatgcc gtttttatgg
     2401 gaaacctcag agcggagcag gaagaggccg tttcggcatt actccgttat gacactggtg
     2461 tgctttgtgc gccaacggct tttggtaaga cagttaccgc agcggcagtg attgccaagc
     2521 ggaaagtgaa tacactgata ctggtacacc ggactgaatt gctgaagcag tggcaggagc
     2581 gtctcgcggt gtttctgcag gccggtgaca gtattggtat tatcggggga ggtaaacata
     2641 aaccctgtgg caatattgat attgcggtgg tgcagtccat atccagacaa ggagaagttg
     2701 aacctctggt caggaattat gggcaaatca ttgtggatga gtgccatcat attggcgcgg
     2761 tttcattttc tgcgattctg aaggaaacga atgccagata tctgcttggc ctgacggcaa
     2821 caccaatccg acgggatggt ctgcatccca ttatttttat gtactgtggt gccattcgcc
     2881 atacagcggt ccgcccgaag gaaagcccac ataatctgga ggtactgatc cgttcccgtt
     2941 ttacatctgg tcatttacca tcggatgcga gaatccagga tattttcaga gaaattgctc
     3001 tggatcatga cagaacggtg gcgatagctg aagaagccat gaaagctttc gggcaggggc
     3061 gaaaagttct ggtactgact gaacgtacag atcatctgga tgagatagca tcagtgatga
     3121 attcactgaa attgtctccc tttattctcc atggtcgact atcgaaaaaa aagcgtgcga
     3181 tgctgatatc cgggctgaat gctcttcctc ccgattctcc tcgaattttg ttgtcaacag
     3241 gcagacttat tggtgaggga tttgaccacc ctccgctgga tacgctgatt cttgccatgc
     3301 ctgtgtcatg gaaagggaca ttacagcagt atgcagggcg tcttcacaga gagcataccg
     3361 gtaaaagcga tgtcaggatc attgattttg tggataccgc gtatcctgtg ctgctcagaa
     3421 tgtgggataa acgtcagcgg ggttataaag cgatggggta caggattata gctgacggcg
     3481 atgaatcagt gatttaattt taaggagtac tttacccttt ttaaataatt tgagctcccc
     3541 gggttgttcg ggcaacaaag agtcccagta tacaggcttc aggaatatgt tgtagtatca
     3601 ggcttttcat cgctttaaaa tgactaccgg cagtgagaac gtcatcaaaa ataacaatct
     3661 agggcctgca cccttccagt aattgttcat ccagttggta gtttgccata atcgcttctg
     3721 gtaagggccg gtttgttgcg gtatgagagg ctattgttga tacgttttgc acaaccagtt
     3781 cccagaaatc cagccatggt tcgtaggttt gcgcaattcg cagaatttct gtcatacggt
     3841 catcgtaagc ggcatctgta cggcatttgg atggtggtac gggaacaaat gtgtattcct
     3901 caagattacc gatatttgat atgagcatct gagcaacata ctcaatagca gcctttttat
     3961 agtggtaatc aggctacgaa agtacagcgt tttataaatt gtctcaggtg atattcgcag
     4021 cgttttttga cgtggttttg ttcgccttaa ccatcctgat atttgctctg gagaccattt
     4081 catctccagc ttttccagaa caagctttcg caatggtaaa ttttgatcca gtaagcacgg
     4141 ttttggcctt ttcgccattc tgttggctcg gttattagca tcaacagctt tgtaatagcg
     4201 tctgccccga ttacg
//
LOCUS       DAZGOB010000240         4147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGOB010000240 DAZGOB010000000
VERSION     DAZGOB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.241"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..943
                     /locus_tag="ACHLIF_005003"
     CDS             <1..943
                     /locus_tag="ACHLIF_005003"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HFO9608824.1"
                     /translation="KKINKEIFEKIDYPHYLHGALSGRDYISNAAVHTRKRTVICLDI
                     TNFYPSISKKDVCSIFKNLMRFSPEVSLCLTELVTLNNKVPQGGGCSSYIANLLFFNS
                     EYNLYNRLKSMGLSYSRLLDDITISSDKDLSSEEKTKVIKLVHGMVNQYRLSINESKT
                     TIEHSKDSSSKLSVTGLWVKHGVPKLTKENRRYIRYLVYICKKQGAYERHTKEYHDLW
                     NRCSGKVAQMSRLGHVQAVELRAILSEIMPVYDDYKISKLKLMAKHYLNKFTPPLTDE
                     QIRKIDRMLYDFDIVGRTNKNLAKLYRRKLVALLPDR"
     gene            948..1151
                     /locus_tag="ACHLIF_005004"
     CDS             948..1151
                     /locus_tag="ACHLIF_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Se72 family effector protein"
                     /protein_id="HFO9608825.1"
                     /translation="MENGFVNFYDHVKGYGFIRRERGRDVFFRYDDFLFLGHDVDICK
                     GILVRFKLEKTDKGFKAVAIQKV"
     gene            complement(1988..2182)
                     /locus_tag="ACHLIF_005005"
     CDS             complement(1988..2182)
                     /locus_tag="ACHLIF_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO9608826.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEELQNSIQTRFEAENRTGI"
     gene            2467..2790
                     /gene="mobA"
                     /locus_tag="ACHLIF_005006"
     CDS             2467..2790
                     /gene="mobA"
                     /locus_tag="ACHLIF_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO9608827.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            3040..3378
                     /locus_tag="ACHLIF_005007"
     CDS             3040..3378
                     /locus_tag="ACHLIF_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608828.1"
                     /translation="MNISIKNNDLKTIARFDGKTLRDAFSRKIATFDGKILRDASLKK
                     IATFDGKVLRDASLKKVATFDGKVLRDASLKKVATFDGQNLRDASSKIILSADAEVGI
                     VVMAFVAELI"
     gene            complement(3927..>4147)
                     /locus_tag="ACHLIF_005008"
     CDS             complement(3927..>4147)
                     /locus_tag="ACHLIF_005008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608829.1"
                     /translation="IPFKDLLGSKICLSFLFFPVSTLNCLYDIDTDLTIQSNLFLSMP
                     NIADKESKDATGVPLNLGLFMMFELKVD"
BASE COUNT         1142 a          779 c          988 g         1238 t
ORIGIN      
        1 aaaaaaaata aataaagaaa tttttgagaa aattgattac cctcattatt tgcatggggc
       61 gttaagtggt cgcgattata tatctaatgc cgctgttcat actaggaaaa gaacagttat
      121 atgtcttgat atcaccaatt tttatcccag cattagtaaa aaagatgttt gttctatttt
      181 taaaaattta atgcgttttt ctcctgaggt ttctttgtgc ttaacggaac ttgtgacgtt
      241 gaataataag gttcctcagg gtgggggctg ttctagttat attgcaaatc ttttattttt
      301 taatagtgag tataatctct ataatagatt gaagtctatg gggctttcat attcaaggct
      361 tttagatgat attactatat cctctgataa ggatctttca tcagaggaaa aaacaaaagt
      421 aataaaactc gttcacggaa tggtgaatca gtacagactt tcaataaatg aaagtaagac
      481 aacgatagag cactcaaaag attcgtcttc taaacttagt gtcactgggt tgtgggtaaa
      541 gcatggcgtt cctaagttaa caaaagaaaa tagacgttat atcaggtact tagtttatat
      601 atgtaaaaaa caaggtgctt atgaacgtca tacgaaagag taccatgatt tatggaatag
      661 atgctctggc aaggttgctc aaatgtcgag gcttgggcat gtccaggcgg ttgaattgag
      721 ggcgattctt tctgagataa tgccagttta tgatgattac aagatatcta aattaaagtt
      781 gatggctaag cattatctaa ataaatttac gccgccttta actgatgagc agataaggaa
      841 aatagatagg atgctctatg attttgatat agtagggcgg actaataaga atcttgcaaa
      901 actatataga cgtaagcttg tggcattact tccagatagg tgattatatg gaaaatggat
      961 ttgttaattt ttatgaccat gttaaagggt atggctttat aaggcgtgag cgtggacgtg
     1021 atgttttctt tcggtatgat gattttttgt ttttagggca tgatgtcgat atatgtaaag
     1081 gtattttagt taggtttaaa ttagaaaaaa cagataaagg gtttaaagct gtagcgattc
     1141 aaaaggtata acatcgtgga tgtgtcagct tgtttttatg atatgagaac ttgagatcct
     1201 ttctatctcg tcgtaatctc ttgctgtgta aacgaaaaaa ccgccttgca gggcggtttt
     1261 tcgaaggttc tcagagctac caactctttg agccgaggta actggcttgg aggagcgcag
     1321 tcaccaaaac ttgttctttc agtttagcct taacaggcgc atgacttcaa ggctaactcc
     1381 tctaattcgg ttaccagtgg ctgctgccag tggcgataag tcgtgtcttc tcggtttgga
     1441 ttcaagtcga tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat
     1501 acagtccagc ttggagcgaa cgacctacac cgggccgaga taccaacagc gtgagctatg
     1561 agaaagcgcc acgtttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1681 tgtcgggttt cgccacctct gacttgagcg tcggtttttg tgatgctcgt caggggggcg
     1741 gagcctatgg aaaaacgcct cctgctcgcc cttcttctgg gcctttgtct tttgctcaca
     1801 tgttctttcc ggctttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1861 ctgacaccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg
     1921 aagagcgcct tatgtgacat tttctcctta cgctcctcta tgccgttctg catcctgtcc
     1981 tgatgcgtta tatcccggtg cgattttccg cttcaaagcg tgtctgtatg ctgttctgaa
     2041 gttcttccgc gagttcgtgc agcttctcac acatggctgc ctgttcgtca gcatcaagtg
     2101 cgtccagttt ttcgagcagc gtcaggctct ggctttttat gaatcctgcc atgttgagta
     2161 cggtttgctg ctgcttgttc atctttctgt tttctccgtt ctttctgtca tctgctttgt
     2221 gtgattatat cgcgtaccac ttttcgactg ttttaccgtc ggtattctgc tgtctgactc
     2281 tttgacgggc atttctgtca gacaacactg tcactgccaa aaaactgccg tgcctttgtc
     2341 ggtaattcga gcttgctgac aggacaggat gtgtaattgt tataccgcgc atacatgcac
     2401 gctattacaa ttaccctggt cagggctttg ccccgacacc ccatgtcaga tacggagcca
     2461 tgttttatga caaaacgaag tggaagtaat acgcgcaggc gggctatcag tcgccctgtt
     2521 cgtctgacgg cagaagaaga ccaggaaatc agaaaaaggg ctgctgaatg cggcaagacc
     2581 gtttccggtt ttttacgggc ggcagctctc ggtaagaaag ttaactcact gactgatgat
     2641 cgggtactga aagaagttat gagactgggg gcgttacaga aaaaactctt tatcgacggc
     2701 aagcgtgtcg gggacaggga gtatgcggag gtactgatcg ctattacgga gtatcaccgt
     2761 gccctgttat ccaggcttat ggcagattag cttcccgggg agaaaactgt cgaaaacaga
     2821 cggtatgaac accgtaagct cccaaagtga tcaccattcg ctttcatgca tagctatgca
     2881 gtgagctgaa aacgatcctg acgcatcctt cctgtttttt cggggctaaa cctctctttt
     2941 tgcggtattt gcgtcagaat tgcgttcagc gcgtttcagc ggtgcgtaca attaagggat
     3001 tatggtaaat acaattggtt aaattactat tggtgataaa tgaatatttc aataaaaaac
     3061 aatgacttaa aaactattgc tcggtttgac ggtaaaacct taagagatgc cttttcgaga
     3121 aaaatagcga cttttgatgg caagatttta agggatgcta gcttaaaaaa aatagcgact
     3181 tttgatggca aggttttaag ggatgcctct ttaaaaaaag tggcgacttt tgatggcaag
     3241 gttttaaggg atgcctcttt aaaaaaagta gcaacctttg acggtcagaa tttaagagat
     3301 gctagttcaa aaattatcct gagtgccgat gctgaggttg ggattgttgt tatggccttt
     3361 gtagctgagt tgatttgaat ctcatgctgc atacctttta gcctatcgct ggcaggataa
     3421 ggcgctttct tgttggtgac agaaagataa aaaactttat aataatcagc gaagcgggcg
     3481 aaccacgcct ggctctcgct ctttaacaaa tggttacgtg tatgttgcgc aaggaaaccg
     3541 atgccttgtt tttaaggcga tctcgcatga gatcatgacc ggcctggccg ctaattaatg
     3601 gtgagaacgc ccctgagcgt gtatcagcac taaaagaata gcgacgattg tctggcaatg
     3661 tgagttaaaa gtcccaaaga gccaatagag tttttccttg ctttcctact ggtctggcaa
     3721 aatttcataa ccaataggag ttgcatcatg tttattatca acatcaacat taacgtaacc
     3781 aagagctctt catcttgttc tggagacggt cagatgaaga aaaaaagaga ggttggctct
     3841 ctggaggcac tgaaaaaaac acagtttttg ttttctatgt gcttgattat gtttaaagta
     3901 atagattacg tatcgaaact ttttaactaa tcgactttta actcaaacat catgaataaa
     3961 cccaggttta atggaactcc tgttgcatct ttggattctt tatctgcgat gttaggcata
     4021 gaaagaaaaa gattggactg gatagtcaag tctgtatcga tgtcgtacaa gcagtttaag
     4081 gttgaaacag ggaagaataa aaaagaaagg caaatttttg aacccaagag atctttgaaa
     4141 ggaattc
//
LOCUS       DAZGOB010000241         4130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGOB010000241 DAZGOB010000000
VERSION     DAZGOB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.242"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(37..444)
                     /gene="cesD2"
                     /locus_tag="ACHLIF_005009"
     CDS             complement(37..444)
                     /gene="cesD2"
                     /locus_tag="ACHLIF_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO9608830.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            complement(451..1395)
                     /gene="espB"
                     /locus_tag="ACHLIF_005010"
     CDS             complement(451..1395)
                     /gene="espB"
                     /locus_tag="ACHLIF_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO9608831.1"
                     /translation="MNTIDYTNQVMTVNSVSENTTGSNAITASAINSSLLTDGKVDVS
                     KLMLEIQKLLGKMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGA
                     ISSVLGILGSFAAINSATKGASDIAQKTASTSSKAIDAASDTATKTLTKATESVADAV
                     EDASSVMQQAMTTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAA
                     VDKITGSTPFIAVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQ
                     EVRRAQDDIASRLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            complement(1416..2558)
                     /locus_tag="ACHLIF_005011"
     CDS             complement(1416..2558)
                     /locus_tag="ACHLIF_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFO9608832.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKILGQVFGWLGVAVTAIAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRIVSGRV"
     gene            complement(2571..3149)
                     /gene="espA"
                     /locus_tag="ACHLIF_005012"
     CDS             complement(2571..3149)
                     /gene="espA"
                     /locus_tag="ACHLIF_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO9608833.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISDLNAELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
     gene            complement(3207..>4130)
                     /locus_tag="ACHLIF_005013"
     CDS             complement(3207..>4130)
                     /locus_tag="ACHLIF_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFO9608834.1"
                     /translation="QNELAMITSSSLSETIEGLSLGYRKGSARKEEEGTTTEKLLNDM
                     QELLTLTDSDKLKELSLKNSGLLEQHDPNLAMFGNMPKGEIVALISSLLQSKFVKIEL
                     KKKYAKLLLDLLGEDDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSEKGASLLD
                     WFMEIKDLPEREKHLKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYT
                     DIIAISINKDKDVILNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKDFFA
                     SLPDACFIDREQRDNTLLMIGKVIDYKEDVI"
BASE COUNT         1158 a          970 c          766 g         1236 t
ORIGIN      
        1 taaattttcc tcaatagtta atagaaataa aaatttctaa ctatttacat tcattacgaa
       61 ccaaattccg tcttctgttt cttgctgcac agcgcccttt ctgatgaagt aattaaacaa
      121 ctcttcgcgc ttttgacgtt ctgaatctga accaatctta gatttcatcg cactgatttt
      181 cttaataccc gaaaaatggt ggtacaactc ttcgagcaag cgagtcagtg ataaaacagg
      241 gccgttaagc cctgtttggt tacgtgcttc aaaagagcac aaaatcagtt cttgttcatc
      301 aagtctgtac gctaatgaga aaaaagaggt ctcaatccgc catccgataa aaaaggcact
      361 gccacaaaga aactccttct gtattgtgta ccctttttgt tcaagatagt gattaagcac
      421 ttcatcatta aacgtatcga ccatgatcaa ttacccagct aagcgaaccg attgacccat
      481 acgattctga agatcagtga gatcgcgagc ggctgttgtc atatcacgca gacggctagc
      541 gatatcatcc tgcgctctgc gaacttcttg cttatacaga tccaaattac cctgctggaa
      601 gttctccaca gacttataac gctgttcgct aatctcatgg ttagatttga ctgattcaga
      661 taccgttgtt ggcaatgtct tcgtgccttc ggcaagactg gtaacggcaa taaatggtgt
      721 agagccagta atcttatcta ctgcagccat aaagcggctt gcccgacttg ccttctgagc
      781 ggcatctgca gcgttttcag ccagctgaga agctctctga gcagaatcgg caatgtcatc
      841 agcaacgtcg gatgtacggc tggccgctct cgttgctgta gtcatcgctt gctgcatcac
      901 gctggatgca tcttcaacag catcagcaac gctttccgtt gccttagtca acgttttagt
      961 cgcagtatca gaagccgcat caatagcctt agaagatgta gaggcggttt tttgagcaat
     1021 atcactcgcg cctttcgtag cactgttaat tgctgcaaaa gagcctaaga tccccaatac
     1081 tgatgaaata gccccaccaa ccagagcggc tgttgcagcg gccttttttt catcaatggc
     1141 tttattctgg ctctcaaaaa cggccagttg gatctgatag ctctgcgaca actgttgctg
     1201 ttggtaatcc tgcaatatac gcaccatctt gcccaggagt ttttgaattt ccagcatcag
     1261 tttagaaaca tcgaccttac catcggtaag caaagatgaa ttaatagcag atgcggtaat
     1321 tgcattagag ccggtagtat tctccgaaac agaattaacc gtcattactt gattagtata
     1381 atcaatagta ttcataatta aattctcttt aataattaaa ctcgaccact aacaatacgg
     1441 ctatttactc gtgctgagtc ggatattaac tgagaagcat tttgtaaata gcttgcctgg
     1501 tttcgcaact cggctgcagc tttatccagc tcaaccttcg cactctcacc caggttttca
     1561 ctctgtcgag ttaagttttg taccgcgtaa gcagataact gagccaatat gctgtattgc
     1621 gcagaatcat tattcagcac agttgtccca atagaacgtg cagaggtcgt aaatccattt
     1681 gccgttgccg caacttttga aacaacgttc tcaacaaata catcggccag tttagtaact
     1741 gttttcacga tgtttgaacc aactttgtta gcgacatcgc caactttaga cataagtgaa
     1801 gacaccccgc caatgccggc tgtcagaata cttgcagcaa tagatactcc gccaaagact
     1861 tgcgctgctg tctttaacgc ctgcggggcc ttatcgccca ttacatcaac tgcagtttgc
     1921 agtgccattg ctgttgcgct aacggcaaca acagcccaga gtgccgggtt aaaaatagct
     1981 gcaatagcag ttaccgcgac gcccaaccaa ccaaagacct gaccaagaat tttacttttt
     2041 tgtgctttct cttcggcttt ctgttgttct tcgagctgct ttttaaactc cagcgtttta
     2101 ttatccagcg ccttattttg cccatccgtg tagatctcgt tggagttttt catactcgca
     2161 actttctgcg ctgaagcatc cagggataac agggtgacca tcatcatcat ggcctgaggg
     2221 tcaacgttat tcacctgaga aagataagga tagccagagc ttgcattctg agtgctgcgc
     2281 tgtggttcgg ttccctctgt tcgactccta acttcaccac taataccacc aaacagttta
     2341 ctcaggacat cctcagcaac agagggcgtc actaatgagt ggcctgccgg tggcgttggt
     2401 aaagcggtac tttctgtcca acttgctgat ggggccgtgg atttaaccag ttgtaaatcc
     2461 agcgcggaag tcacttcagg ctgattaata cctgaagtag ccgtagcagc gctggcccca
     2521 gacctcatag actggatatc gctatttaca ttaagcatag ttatctccgg ttatttacca
     2581 agggatattg ctgaaatagt tctgtattgc agtgactgaa tatcagaacg tgcactcgtt
     2641 aataggttta acgtatttga catttgctgt atttcaagct ggctattatt cactaccgtg
     2701 gtcaagttat tcgatttggc cgaaatagcg gccttcaccg tttgcaaatc accagcgcct
     2761 aattcagcat ttagatcgct aataccactt actgtaatgt cattgcgagg atcatttata
     2821 tagtcaatca cttcttgagg aagtttggct ttcttattct tgtcagaact actctgaaca
     2881 tcagcaattt tagcatccac aagattagcc attttttggg ctgtggttga ctccttagat
     2941 gcctcattca tatcagcaaa ctttgcaatc gacagatcgc tttgtgcctg atacatatag
     3001 gcaaacatga gaagcgcagc ctgaaaaaca ccgactttat taaatagctg aactacttcg
     3061 tctttcgaca tactgcctaa gtcatagact gtcgatgtcg aagtactcgc gttcgcacta
     3121 gcaactgatg ttgcagttga tgtatccatg tatatacctc ttaataatct ttcttagcaa
     3181 gaccaaaaac gattaaaaaa ccaatatcaa ataacatcct ccttataatc aattacttta
     3241 ccaatcatta ataatgtatt atctctctgt tccctatcaa taaaacaagc gtcaggtaat
     3301 gatgcaaaaa agtctttcaa taggtggaaa taataaacat gtttatcttc gacaatcgat
     3361 acccgagaag ggctttccag gagccagtct tctgttaacc atacatgttc aataatagac
     3421 aatacttcat ttaaaatgac atctttatct ttattaatag agattgcaat aatatctgta
     3481 tagttatcaa gcgataaaaa aatgattacc cggcataaat cgctaataat tgaagatgtt
     3541 ttcaccttat cttcaaaaga agacatataa gacagatcga acgatagcgc cctaataata
     3601 acctttaagt gcttctcacg ctctggaaga tccttaatct ccataaacca gtcaagtaaa
     3661 gaggctccct tttcagaatc ctcatccttg gccttttcat aaagcttttt gatcttctgg
     3721 ataccttcct gatttaactc acccacccct aaccaggaaa gcagagccaa ctcccaatca
     3781 tcttcgccta ataaatctaa taataatttg gcgtatttct tttttaattc aatcttaaca
     3841 aacttagatt gtaataaaga agatataagt gcaacaatct ccccttttgg catattacca
     3901 aacatagcca aattaggatc atgctgttcc agcagcccac tgttctttaa tgatagttct
     3961 tttaatttat cgctatcggt cagagtaagc agctcttgca tatcattaag cagcttctcc
     4021 gttgtcgtac cttcctcttc tttccttgca ctgcctttgc gatatcccag gcttagccct
     4081 tcaatcgttt cagaaagcga tgaactagta atcatcgcta attcattttg
//
LOCUS       DAZGOB010000242         4060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGOB010000242 DAZGOB010000000
VERSION     DAZGOB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.243"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1820
                     /locus_tag="ACHLIF_005014"
     CDS             <1..1820
                     /locus_tag="ACHLIF_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9608835.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDIGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1817..2023
                     /locus_tag="ACHLIF_005015"
     CDS             1817..2023
                     /locus_tag="ACHLIF_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO9608836.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2020..3621
                     /locus_tag="ACHLIF_005016"
     CDS             2020..3621
                     /locus_tag="ACHLIF_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9608837.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSNEQRDKLAGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            3602..>4060
                     /locus_tag="ACHLIF_005017"
     CDS             3602..>4060
                     /locus_tag="ACHLIF_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9608838.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDI"
BASE COUNT          925 a         1053 c         1254 g          828 t
ORIGIN      
        1 ccaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
       61 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgcgaggtg aatgtggtga
      121 agtctgcccg tgttggttat tccaaaatgc tgttgggtgt ttatgcctac ttcatagagc
      181 ataagcagcg caacaccctt atctggttgc cgacggatgg cgatgccgag aactttatga
      241 aaacccacgt cgagccgacc atccgtgata ttccgtcgct gctggcgctg gccccgtggt
      301 atggcaaaaa gcaccgggat aacacgctca ccatgaagcg tttcaccaat gggcgtggtt
      361 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcagtg gatgtggcgg
      421 gttatgatga acttgctgcc tttgatgatg atattgaaca ggaaggctct ccgacgttcc
      481 tgggtgacaa gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
      541 aagtgagagg cacctgtcag attgagcgtg cagccagtga atccccgcat tttatgcgtt
      601 ttcatgttgc ctgcccgcac tgcggggagg agcagtacct taaatttggc gataaagaga
      661 cgccgtttgg cctcaaatgg acgccggatg atccctccag cgtgttttat ctctgcgaac
      721 ataatgcctg cgtcatccgc cagcaggagc tggacttcac tgatgcccgt tatatctgcg
      781 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
      841 ttgagccgcc ggacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
      901 gggtgcagat tgtcaaagac tggatgaaaa cgaaagggga tacgggaaaa cgtaaaacct
      961 tcgtgaacac cacgctcggt gagacgtggg aggcgaaaat tggcgaacgt ccggatgctg
     1021 aagtgatggc agagcggaaa gagcattatt cagcgcccgt tcctgaccgt gtggcttacc
     1081 tgaccgccgg tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc
     1141 cgggtgagga aagctggctg attgaccggc agattattat gggccgccac gacgacgagc
     1201 agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag
     1261 aaatgtcggt atcccgtatc tgctgggata ttggcgggat tgacccgacc attgtgtatg
     1321 aacgctcgaa aaagcatggg ctgttccggg tgatccccat taaaggggca tccgtctacg
     1381 gaaagccggt ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa
     1441 ttggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg
     1501 atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatctgaccg
     1561 aagcgcagca gctgacagct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa
     1621 tactgtggga cagcaaaaaa cgacgcaatg aggctctcga ctgcttcgtt tatgcgctgg
     1681 cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcgctg ctggcgagcc
     1741 tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagattac gcccgtgcct
     1801 tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca
     1861 tgacctgatg acaggtaaac gggtggcaac agtacagaaa gacggacgaa gggtggagtt
     1921 tacggccact tccgtgtctg acctgaaaaa atatattgca gagctggaag tgcagaccgg
     1981 catgacacag cgacgcaggg gacctgcagg attttatgta tgaaaacgcc caccattccc
     2041 acccttctgg ggccggacgg catgacatcg ctgcgcgaat atgctggtta tcacggcggt
     2101 ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgagtgaaag tgtggatgca
     2161 gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt acgcaataac
     2221 ggctatgccg ccaacgccat ccagctgcat caggatcata tcgtcgggtc ttttttccgg
     2281 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     2341 tcccgcgagg ttgaagcggc atggaaagaa tttgccgagg atgactgctg ctgcattgac
     2401 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     2461 aacggagaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     2521 cagttccgga tggtcagccc gaagcgcatc agcaatccga acaataccgg cgacagccgg
     2581 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     2641 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     2701 ggcgggcgcg cctcgttcat tcacgtcttt gaacccgtgg aggacgggca gacccgcggt
     2761 gcaaatgtgt tttacagcgt gatggagcag atgaagatgc tcgacacgct gcagaacacg
     2821 cagctgcaga gcgccattgt gaaggcgatg tatgccgcca ccattgaaag tgagctggat
     2881 acgcagtcag cgatggattt tattcttggc gcgaacagta atgagcagcg ggacaagctg
     2941 gcgggctgga ttggtgaaat tgccgcgtat tacgccgccg cgccggtccg gctgggaggc
     3001 gcaaaagtgc cgcacctgat gccgggtgac tctctgaacc tgcagacggc tcaggacacg
     3061 gataacggct actccgtgtt tgagcagtca ctgctgcggt atatcgctgc cggactgggt
     3121 gtctcgtatg agcagctttc ccggaattac gcccagatga gctactccac ggcacgggcc
     3181 agtgcgaacg agtcgtgggc gtactttatg gggcggcgaa aattcgtcgc atcccgtcag
     3241 gcgagccaga tgtttctgtg ctggctggaa gaggccatcg ttcgccgcgt ggtgacgtta
     3301 ccttcaaaag cgcgtttcag ctttcaggaa gcccgcagcg cctgggggaa ctgtgactgg
     3361 ataggctccg gtcgtatggc catcgatggt ctgaaagaag tacaggaagc ggtgatgctg
     3421 atagaagccg gactgagtac ctacgagaaa gagtgcgcaa aacgcggtga cgactatcag
     3481 gaaatttttg cccagcaggt ccgtgaaacg atggagcgcc gcgcagccgg tcttaaaccg
     3541 cccgcctggg cggctgcggc atttgaatcc ggactgcgac aatcaacaga ggaggagaag
     3601 agtgacagca gagctgcgta atctcccgca tattgccagc atggctttta atgagccgct
     3661 gatgcttgaa cccgcctatg cgcgggtttt cttttgtgcg cttgcaggcc agcttgggat
     3721 cagccgcctg acggatgcgg tgtccggcga cagcctgact gcccaggagg cactcgcgac
     3781 gctggcatta tccggtgatg atgacggacc acgacaggcc cgcagttatc aggtcatgaa
     3841 cggcatcgcc gtgctgccgg tgtccggcac gctggtcagc cggacgcggg cgctgcagcc
     3901 gtactcgggg atgaccggtt acaacggcat tatcgcccgt ctgcaacagg ctgccagcga
     3961 tccgatggtg gacggcattc tgctcgatat ggacacgccc ggcgggatgg tggcgggggc
     4021 atttgactgc gctgacatca tcgcccgtgt gcgtgacata
//
LOCUS       DAZGOB010000243         4018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGOB010000243 DAZGOB010000000
VERSION     DAZGOB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.244"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..625)
                     /gene="espL2"
                     /locus_tag="ACHLIF_005018"
     CDS             complement(<1..625)
                     /gene="espL2"
                     /locus_tag="ACHLIF_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO9608839.1"
                     /translation="MPIINKSASNYVEYISKNNPPYLSKKRDASINLNGKVSDCNGEI
                     IWCRHIASYWSEFFCSNSGKIDYETFSSPQLLSKAIVIQENKGTNNIKGDVYFVENES
                     WGSVIYNLFLQLEKENKSHTSLEVHSPGHAMALGIKIKNDKENKFVINFYDPNQTATH
                     KRVFFCTNNICDIINLTAYDFLSEQCLKCYGLKEDTLSLFVDKTKSND"
     gene            complement(616..1179)
                     /locus_tag="ACHLIF_005019"
                     /pseudo
     CDS             complement(616..1179)
                     /locus_tag="ACHLIF_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(1413..2090)
                     /locus_tag="ACHLIF_005020"
     CDS             complement(1413..2090)
                     /locus_tag="ACHLIF_005020"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO9608840.1"
                     /translation="MWHYLHYKVSRGKPHAECDAKRRAGSPPGPDAHQLQRSQHKKSR
                     QQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYEQRWLIEEYHKAWK
                     SGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGSISEETQNDSCEKILTPTEWKLLWV
                     KLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGWFRHQDMAEGYLVM
                     KSLDQEI"
     gene            2288..3907
                     /locus_tag="ACHLIF_005021"
     CDS             2288..3907
                     /locus_tag="ACHLIF_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HFO9608841.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGAPHV
                     GIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSPKKSPDPEPSV
                     PAASLRRPERLKQQTRKKRHQRWTEVMALHNKECSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLDPWREWLKEQRESSNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLA
                     LEFYRILKTQNKSQLSSWFNRVHESGSAEFRRVAAGMEADAAAICEAISSRWSNGVVE
                     GHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
BASE COUNT         1017 a          971 c          910 g         1120 t
ORIGIN      
        1 tgtcattcga ttttgtttta tccacaaaaa gtgacagtgt gtcttctttt agcccataac
       61 atttcagaca ttgctcacta agaaaatcat atgctgtcag gtttattata tcacaaatgt
      121 tattagtgca aaaaaatact cgtttatgcg tcgcggtctg attgggatca tagaaattaa
      181 taacaaactt attttccttg tcgtttttta ttttaatgcc cagagccatt gcatgtccgg
      241 gagaatgaac ttctaatgat gtgtgagatt tattctcttt ctcaagttga agaaaaaggt
      301 tatatataac gcttccccaa gattcatttt caacaaaata cacatcaccc ttaatattat
      361 ttgtcccctt gttttcctga attacaatag ctttgcttaa taattgaggg gatgaaaatg
      421 tttcgtagtc tatctttcct gaattactac aaaaaaactc actccaatat gatgctatat
      481 gtcgacacca tatgatttcc ccattgcaat cactaacctt tccatttaga tttatactgg
      541 catctctctt ttttgataaa taagggggat tgttttttga aatatattca acataatttg
      601 atgccgattt gtttattatt ggcatatgtt tgtatccttt ctataaattt tccgcctgac
      661 agtggaaata atatattcta ctgatttttg tagtttaaac gatttatgaa gaattttact
      721 gttgtacagt accagatact cagccagttt cagattgaac atattcagct attcaaagag
      781 caattcttca ttaaactcaa taaattgttc cctcagtgtt cggttaaaat gctcacttat
      841 ggcatttatt ttcggcgtat agagataagt ccatacgaac ccgacggtgg cttcctgtag
      901 cgttttgtcg aattttcccg agaactcttt gcccatcatc agtgatgatc tggttgatac
      961 caacaaggaa caactgggct acacagcaga agaaatgcct gatgatatca cttttaagcg
     1021 atagtacggc cagagagtag ttgctgtgtt cgtcgagcat ggtaatgaaa taacggtaca
     1081 gctcgccatt ctgagctcaa tagtatccat gccactgagc ttacaggtct ttatcgggcg
     1141 atagtgcttt attcttcggg gctttgtgga tgatttttcg aaatgttcat gatagttagg
     1201 taaagctacc gcatatcatt tgcaaaatga cagatagata tacttcaatt tgaatcttaa
     1261 caattagtat gggaatgctg tcaatcctct gtatgcgtcc accacatatt gaaatttttg
     1321 ttctctaata tattccacgg gcgtcaagag tgtgaggcct gtgctgttat acagcaccag
     1381 acgttgtccc ccatctttga cctgtctctt gatcagatct cctgatcaag agacttcatc
     1441 accaggtaac cctcagccat atcctgatgt ctgaaccagc catcccacat aactacccaa
     1501 ccggggctgc ttgtgcgttt gctgtcatgc catcgcccca gtttagccag tttcaaacag
     1561 gcccatttca gggtcggtgt ttgcgctgga agctgctttc cttccagctt aacccacagt
     1621 agtttccact ctgtcggcgt cagtattttc tcacagctgt cattttgtgt ttcttcactg
     1681 atacttccct gccgcaggcc cagcacccgc accgcgataa ccaccatgcg ctcaaggtta
     1741 tcccgggtct gcattcgcag cgattccaca catgtaccac cacttttcca cgctttgtgg
     1801 tattcctcta tcagccagcg ttgctcgtaa tggctgacga tacgtcgcgc atcggcggca
     1861 ctcgccactt tttctgatgt cagcggatac cagcaggctc cgtcctctcc tgctccccgc
     1921 aacagacata cgtgagcgag agcgcctggc cgctgttgtc gggatttttt atgctgactt
     1981 cgctgtaact gatgagcatc cgggcctggc gggctgcccg cccgcctttt tgcatcacat
     2041 tcagcgtgtg gcttcccgcg gctgacttta taatgcaggt aatgccagat atccgcttcc
     2101 cggtcacaga cagtgattac ctgtttctgt atctccccca gccgttcggc catgcgctcc
     2161 gaagcctgct gccagcggta actttctttt tctgtcacga acggtgcaat agtggttctt
     2221 ccgcaacttg ggtggaagaa aatggatgaa actttctggt gaacaaagat ctaagctgtt
     2281 ttaagtcatg ggcaactcaa tgcactctct taagacactt ctacagttac cttgcggatg
     2341 gcgatgcagt cgacaaatta ttagctctga cggtataacc ctccatctcc acggaaaacg
     2401 caaaaaagca caatgtcctg aatgctttaa gcgtagcgac tctgttcata gttgtcgtcg
     2461 gcgccggata cagcatctac cctgctcagg acatacgcta tggcttgtat tttccgtccg
     2521 ccactggtac tgccgtaacc cggcttgttc acgtaaaatt tttgccgggt cgcttgctcc
     2581 cttcgccggt tcacaccaac agtcttcaca ggcgttacaa aatttacaac gtcaactggg
     2641 attaatcgcc ggaggtgagg ccgggaaacg ggcagcaacg gcagtggggc tccgttgcag
     2701 tgctgatact ctccttcgca gggttatcaa taccccggag acgaaacagt caggcgcgcc
     2761 tcatgtcggt attgatgagt gggcgtggca tcggggccac cgttacggta cgttaatcgt
     2821 caatcttgat actcaccgtc ccctcgtcct gcttcccggc cgtgatcagc gtacgctggc
     2881 gacctggttc agaaaatatc cggaaataca aattgtctcg cgtgatcgca gtggagtcta
     2941 tgcgacagca gcacgtgaag gtgcacctca ggccagacag gtggccgatc gatggcatct
     3001 gctaaaaaat attggcgatg cgcttgaacg aatgatgtac agacatatac ctctgatacg
     3061 tcttgttgcc agtgagttgt caccaaagaa atcacctgat ccagaaccat ctgtgcctgc
     3121 agcatcgctc cgtcgtccgg aacgcctaaa acaacaaacc cgcaaaaaac ggcatcagcg
     3181 ttggacagag gttatggccc tgcataacaa ggaatgtagt ttcagggaaa tatcccgtat
     3241 tacaggcctg tcgcgtgtga cagtcagtcg ctgggtgcgt tcaggaacat tccctgaaat
     3301 gtcaacccga cctccaaagc gagggcttct ggacccatgg agggagtggt taaaagagca
     3361 acgagaaagt agtaattata acgccagccg gatatggcgg gaaatggtgg cccggggatt
     3421 tacaggcagt gaaaccatcg tcagggatgc ggttgccaaa tggcgtaaag gctggaaccc
     3481 accggttact actgccgtca gacttccttc agtgtcccgg gtaagccgct ggttgatgcc
     3541 ctggagaata accagggatg aagaaaatta tgcttcccga tttattagtc tgatgtgtga
     3601 aaaagaacct gagctgaaaa tagcgcagca actggcactc gagttctacc gtattctgaa
     3661 aacccaaaat aaatcacagc taagcagttg gttcaatcga gtccacgaaa gcggttcagc
     3721 agaatttcgg cgcgtggcgg cggggatgga agctgatgct gcagctatat gtgaggcaat
     3781 cagtagtcgc tggagtaatg gtgttgtcga aggtcatgta aatcgcctga agatgctgaa
     3841 acgccagatg tatggtcgag caggatttga actactcagg cagagggtaa tgagtccact
     3901 ggcatgaaaa tctccaccca ggttgcggaa gagccacttt ccgtcgttga cactgacgac
     3961 atcttcctgc tgaaacagcg cctggccgaa caggaagcgc tgatccacgc cctgcagg
//
LOCUS       DAZGOB010000244         3989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGOB010000244 DAZGOB010000000
VERSION     DAZGOB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.245"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..144)
                     /locus_tag="ACHLIF_005022"
     CDS             complement(<1..144)
                     /locus_tag="ACHLIF_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608842.1"
                     /translation="MYNFITIMYDVFSCFGVLAKNQNSRDIRNIKNFSSHQHSLGDMF
                     DELI"
     gene            341..1077
                     /locus_tag="ACHLIF_005023"
                     /pseudo
     CDS             341..1077
                     /locus_tag="ACHLIF_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1256..1552
                     /locus_tag="ACHLIF_005024"
     CDS             1256..1552
                     /locus_tag="ACHLIF_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9608843.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1860..1976
                     /locus_tag="ACHLIF_005025"
     CDS             1860..1976
                     /locus_tag="ACHLIF_005025"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608844.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            2195..3628
                     /locus_tag="ACHLIF_005026"
     CDS             2195..3628
                     /locus_tag="ACHLIF_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO9608845.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
BASE COUNT         1225 a          752 c          867 g         1145 t
ORIGIN      
        1 tattaattca tcaaacatgt cgcccagtga atgttgatgt gaggaaaaat ttttaatatt
       61 tcggatgtca cggctattct ggtttttagc cagaacacca aaacatgaaa agacatcata
      121 cattatagtg ataaaattat acataacatt ctccttttat tgcactatat aaaattttaa
      181 cattatatca ttttgctttt ctgttaactg gttttcattt ttgaatcaat attttctgaa
      241 cagggtaata gtacacgtat taacagatta tgtcagaagg ccccggtgat gccttctgac
      301 gatagattaa tgttgtattc tattggtatc tggagaccat atggcaacag ttacagtata
      361 ttctccccgc tgtaattcct ataaagtgta ccgttatgat cacagcacct cagatcatga
      421 gcgtttccag tgctaatcct gcaaacgtgt atttcaactg aattacatct acgaaacccg
      481 aaaaccaggc attaaagagc aaattgttga aatggttcat aacggagccg gggttcgcga
      541 tagctccaga acgctgaagg tggatatcaa caccgtcatt ctcactttaa aaaacgctca
      601 ccacgtaaag taaccatatc tccagccgcc agtgtcgatg tggcacttat ctgtgaactt
      661 gatgaacaat ggagttttgt cgaaaacaaa gctcgtcagc agtggcactg gtacgcgtat
      721 aagaccaaag ctgacggtgt gctggcttac acttttggtc ctcgcactga tgaaacatgc
      781 cgtgagttgc cggaattcct gaagccattc tccgcaggca tgataaccag agacaaccgg
      841 agcagttata ccagagaaat gccacaggat aagcatcttg tcggcaagat atttacccgg
      901 cgcattgagc gcaacaatct gacacttcgt actcacatca aacgacctgc ccggaaaacg
      961 atttgctttt tacgctccct ggaaattcac gaaaagccat tggttcattt atcgaaaaca
     1021 catgtactac tgacaggagt cattaccagg gcctcttttg ctgttttttt gccatgaatg
     1081 ataatgagaa tcgacagcga taagggaaaa gtaaaataac actaatgctg tcgttttcta
     1141 tcatattgat ttttaattat ttattgtctt ttttctcaac ccattttttt acgcagacaa
     1201 atttacccgg ggaatacaaa ctgtaacgac agttcaccac actatggggg ataaaatgcc
     1261 agatcaatcc ggacactgca ggactccttc acgcatctta cggatgcctg aactgagcca
     1321 attgttaggt atatctcgtt cgacaattta cgaaaaaatg aatccgttat cgaaatacta
     1381 cgatgcaacc ttccccaggc cggtacgtct tggtagtggt tctgtgggct ggcgttcatc
     1441 agctattgac gaatggctta ctttacatac tgtacctgcc agatcagcag tcaaaggtgt
     1501 taatgacgaa gtgagacata cgctttcttc agatatgacc atgagcagat aagtttttgt
     1561 catcacgtga tcacctcagg aggaaaaaga agcataagaa caatttagcc agaagtttaa
     1621 ttgataaaca gaatttccag cattcattgt tgaaagagca gctattgtcg ctattgtagc
     1681 ggtaattgct gctataaccg aaacgtatta cgaaaaagga agcatttatg ccttacatca
     1741 aaccggatat gtcgtattca gtatatgcat gaccatcaat gatagggaag gcaataatta
     1801 aagctcaata caataaacaa gtacctctga ctctggttgc aatgtcagta ttaacagcga
     1861 tgtcaattgc ctgtcagaat caggttgacg tatgcagtcc cggaaattta cgtggggccg
     1921 ttaatattta cactatggtt ctggagaaag ggaaaagacc gggggaaagg tattagtgaa
     1981 ataatttccg gaggtattaa gaatgagaaa tatgaatgat atttcgtggt gaataataca
     2041 gtcttcactg aattaacctt atcggtttat taaacatcag atctacaaat gctctgtatt
     2101 tctcagagca tcaggtctga atagcagcta ttttcgctat tgtagcggga tttgctgcta
     2161 ttttactaac ccattaatga aaagagaggc ttttatgtgc ttacttgcac cagaaaatcc
     2221 ttatccgata tatgcactac caccgctggt gagaaatgca ataattgaaa ctcaaaaaaa
     2281 tacacaggca cctttggcta tggtggcaac atccgcatta acagcgatct caattgcctg
     2341 tcagaatcag attgacgtgt gcagacctgg aaatttacat gggcctgtta atctttactc
     2401 tctgattctg gctgattccg gtgaaaggaa aacgaccgtg gataaagtgt ttatgaaagc
     2461 attttatctc agggatgaag ccctggcgga tgaatacgcg aaactggttg agaattacag
     2521 tacagaaaag gaaatatggg agcaaaaaca aaaagcgctg gaatcaaaat ttcataaaga
     2581 gattcgtgcc ggtaaagatt acaaggcaac agaatcagag cttgaaacgc atctgaataa
     2641 acctcctgtt ccgccgcaga tacgtcgaac aatttttaat gagacaacga tagagggaat
     2701 gttaaaatat tactccgata gcaatcgctc ttttgctctt gtatccagtg aagggggggt
     2761 aatttttgac agccgggcca tgagtaaact gggaattatt aacactttgt gggatggagg
     2821 ttctcttttc atcgacagga aatcatctcc cggaattaat ttgaaggagc caagactgac
     2881 gatatcggtg atgattcagc ctgatgttta ccacaaaggt ttttgtacgc gaaaaaaaga
     2941 aattgtgaaa acgtcaggac atcatgcaag gtttttgatg tgtcaaccaa catcaacgca
     3001 ggggacaagg ataataaccg gagataatta ttcatcacag tatcaggatt tgtttgaaga
     3061 gaggatcaat gagcttattg atgaaagtct ggcgatgagt ggtgaacgac gttgccttca
     3121 cttttctcct caggctgcca gaatctggac ggattattac aatgatgtgg aatcgaagct
     3181 ggggggattg gggcctttaa gacattgccg ggaatatgct gctaaaaatg cagagtatat
     3241 ggcaagactg gcaggactta tttaccattc gagcggtgaa gagggggaga tttcccctta
     3301 cactgcagaa atggcgagag aattagcaat atggtacggt aatgagtatg tgcggttgtc
     3361 taatccgtta acttttgaca actctgccct gaccgtacct gtgcgactca ttccagagga
     3421 gcttgaactt ttcaactgga taaaaagcta ttgtattgag aaggggatcc tctgtatgaa
     3481 aaaaaatgac attttacagc gtggtccgaa ccgtttccgg aaaaaggata aaatcaactg
     3541 gttactggat ttactgtatg aacaaaacag agttgtaccg gttattgagg gaaaaacgtt
     3601 gtgtgtcgca cctaactttg acctctgaac gacttaatcg ttgagtacat atagtctcct
     3661 ctgctacttg cggaggagac taatgtcttt accatgtcag cgttgtatta gaaaactctg
     3721 gtacaggtca gtcagtaaat ataactggtg taaccactat ttactattgg atagagtgtt
     3781 actcaggata ttgtcggctt tagtctgagg tgaggtgggg ccttcattgt tttcgtgttc
     3841 aataaataat ttaggttaat tagtattatt agtggaagtt atgtgatctg ttatgttgtc
     3901 ttgtacgcac ttaacactgg ttacgtaccg gaacactatt agagtatgtt atggctctgt
     3961 tacagtttat aggtatgaaa aaatataat
//
LOCUS       DAZGOB010000245         3969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGOB010000245 DAZGOB010000000
VERSION     DAZGOB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.246"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..138)
                     /locus_tag="ACHLIF_005027"
     CDS             complement(<1..138)
                     /locus_tag="ACHLIF_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9608846.1"
                     /translation="MTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YE"
     gene            complement(385..858)
                     /locus_tag="ACHLIF_005028"
     CDS             complement(385..858)
                     /locus_tag="ACHLIF_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608847.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNENAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            complement(1013..1606)
                     /locus_tag="ACHLIF_005029"
     CDS             complement(1013..1606)
                     /locus_tag="ACHLIF_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO9608848.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIQTGD
                     AERILSRGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGAAHQQSPEHRS"
     gene            complement(1644..1853)
                     /gene="hha"
                     /locus_tag="ACHLIF_005030"
     CDS             complement(1644..1853)
                     /gene="hha"
                     /locus_tag="ACHLIF_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO9608849.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(1899..2360)
                     /locus_tag="ACHLIF_005031"
     CDS             complement(1899..2360)
                     /locus_tag="ACHLIF_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO9608850.1"
                     /translation="MLKYISLVLFIFSWPVLSADIHGRVIRVLDGDTIEVMASREAVR
                     VRLVNIDAPEKKQNYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFLVRAGAAWVYEQYNTAPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(2362..>3969)
                     /locus_tag="ACHLIF_005032"
     CDS             complement(2362..>3969)
                     /locus_tag="ACHLIF_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012552789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO9608851.1"
                     /translation="EKVLNYMNIKFHGIIATMYLSFVYTAHAEDTNIINGENNSIHGN
                     NNYISGNNNTIVNNIHNNNNRIIGNNNYLNYKAYNNTIIRDGVTVNPPPTQNSGDSYG
                     NVIIGSGDAFSVTSRNIIIGDDARPLEPLGNTSGALIIGQSARAKAYGTAIGHDSKAT
                     GHMSVALGDHASASGYLSVALGWNSQAKQDREMNIGNRFISGLRDGSAATDASTVGQM
                     QRSDAETLSNSKSYTDTRLTDISKTINDRITSTEKLLNDTITAKIRDETTARTQAINA
                     ESARTDSLLLHEQNARKQGDTSTLQSANNYTDSKISDTKAELNTSIDNVTDRAISSAN
                     SYTDSKISDTKAELNTSINKAKNDTISTSNSYTDNKISDTKTELNTNINNAKNEAIST
                     SNNYTDKKYQQGISYTDGKYEQSIQYAQNATEKAEQNANNYTDNRFNKLSNQLNQRFE
                     QLNSKIERAEKRLNAGIAGVTAISSIPYVAENSFSYGIGVGNYQNGNSIAAGIQYKTS
                     LNTNVRLNVSWDSSHNTALGAGLAGGW"
BASE COUNT         1022 a          918 c          755 g         1274 t
ORIGIN      
        1 ttcgtaggcc agagttacct gcaccactgt atttcccctg tgaatattca gctcacataa
       61 ccgttccctg aatatcagtg aaaaacacaa caccgtggcg cacacggcga gcaccccgat
      121 aagggcatac ttcgtcataa actttgacct ctctgcaagg aagaggctac aatcagtagt
      181 gcaatgtact tcatgcagac ctcacgagtt aatggattaa caagtggggt cttcgcattt
      241 ctggtacctg cgtcttgcct gaaccagaaa ggctcagaca cccgccgcga taataccaca
      301 ttccctgttg ctgactatta cctgcattgc ccgcttttta ctacagaagc acgaaggcgt
      361 cactccagcc aataagtatg ctttttattt acatccattt ttaatccggt accgacgttt
      421 ccctccagtt aactcgtttt tcgtatgact catggcaata aaattttctt taccatatga
      481 caacatccca ataatatcaa agttgtcttt taatttgatg gcatcctttt cacttacagc
      541 gacaataagt tgtttaccaa attttctgac catcattgca aactgtgata ctgctgatcc
      601 accacctaaa tcaggccacc cagcacaatt aaaaatgatt gtctgctctt cactattcat
      661 tgcatccctg ataaaggaga cagcattctc atttctctct acatctgaat cactgttaat
      721 ctgagtgaaa tcataatatt tagaacctgg tatagaatac cccttaaaaa acttatcaat
      781 gtgggaggca gagttcccaa caataagaat attgtgttca cgcttaatca tcaattctga
      841 tacatcatca tatttcatat attcaccatt attatataaa gtttgggtac aggtagttcc
      901 ggaatttact ctgtgaccac catttcccgt tttttattac agaaacgccg gggacgggga
      961 aaacttgctt tttcccgttt ccggggttgg acaactgagc aacgcgaagg cgtcagctac
     1021 gatgttccgg tgactgctga tgagcagccc ctgttgtatt cagccattct ccggtcatct
     1081 gcagcagttt tctggcatcg tggcttctga gcaacgggat accagcctgc ctcagaacct
     1141 cttccagaag aatatcccgg cgccggcgtt ccggtcgtaa atggctggca tcatccagct
     1201 ctactgcagc aacaatactg aacgaacgcc gttcaacgat aaccacatca acatgccact
     1261 gagacaccat cctgaataac tgccaccact ggcgtgatcg tggccggata ttcccgttca
     1321 gttggacgat gtccgcgacc cgtacctgtg gacacagata ccaccgcttc atatccacaa
     1381 ttctgaacaa tcctttcata aaactgactt ccctttctgt cagaagcgtc ttctgacgtt
     1441 gccagtattc acctctggac agtattcgct ctgcatcacc ggtctgtata cctgccgcat
     1501 gcaattgcat cagcaaagga gaatgctgat ttacacttct gaggataatc cgaataagta
     1561 gcgctgcaat aatcagaaca atgagaataa aaataatcaa cttcattatt tatctgctcc
     1621 tggcaattaa ttatgctgaa ttattacctt acatatttcc atatctcttt tggtatcctg
     1681 tcatataact ttccggtgat taactccgct aaccggtggt ctgctgctga attaaatgat
     1741 tcacgttcac tggttgataa actgtctctg tttttatgaa taattttttc cagcgtattg
     1801 actgatgaac atcgccgtaa ctgatacagc cattcctgtt ttgtttttgc catttttatt
     1861 cccgctgaga acagatgaat gataaaatta ccagtatttt atttacggtg tctccatatc
     1921 cagggtggaa ccggatcagc atctgaccag agcccacgtt tttgttgtct ggcttcgttc
     1981 tgaaggacag gcagcacggg agctgtgttg tactgctcat atacccaggc agcgccggcc
     2041 cgaaccagaa actgattaac attcattccg tctggtgcat aaacctgccc gagaatacga
     2101 ccataacgat ctcgctgaaa atatgtgacg gtaaccgttt ttcctgcaac cagtgatttc
     2161 atcatgtctg tcgaccaccg gccataattc tgtttctttt ccggcgcatc aatatttacc
     2221 agcctgaccc gtactgcctc ccgtgaggcc attacctcga tggtatcacc gtccagtaca
     2281 cgaataacac gcccgtggat atctgcagat aatacaggcc atgaaaaaat aaataatacc
     2341 agtgaaatat atttcagcat attaccatcc cccggcaaga cctgcaccaa gagcagtatt
     2401 atgtgatgaa tcccatgaga cgttaagtcg cacgtttgtg tttaatgacg ttttatattg
     2461 aatgcctgct gcaatcgaat ttccattctg ataattacca acaccaatac catatgagaa
     2521 actattctca gcaacatacg ggattgatga tattgctgtt acacctgcaa tacctgcatt
     2581 aagacgtttt tctgcacgtt caattttact gtttaattgt tcgaaccgct gatttaattg
     2641 attacttaac ttattgaatc ggttatctgt gtagttattc gcattttgtt cagctttttc
     2701 ggttgcattc tgagcatact gtatactttg ttcgtatttc ccatcggtat aactgatacc
     2761 ttgctgatat tttttatctg tataattgtt tgatgtactg attgcctcat tttttgcatt
     2821 gttgatattg gtattcagct ctgtttttgt atcactgatt ttgttatcag tgtaactgtt
     2881 ggatgtactg attgtgtcgt tctttgcctt gttgatactg gtgttaagtt ctgccttcgt
     2941 atcactgatt ttactgtcag tgtagctgtt tgccgagctg atggcgcgat ctgttacatt
     3001 atcaatgctg gtattcagtt ctgctttcgt gtcactgatt ttactgtcag tgtaattatt
     3061 tgcagattgt agtgtgctgg tatccccctg tttacgtgca ttctgctcat gaagaagaag
     3121 actatcagta cgtgccgatt cagcattaat ggcctgggtt cgtgctgttg tttcatccct
     3181 gatttttgct gtaatggtat cattaagcaa tttttctgta cttgtgatcc tgtcattgat
     3241 tgttttactg atatcagtca gtcttgtatc tgtataactt tttgagttac tcagcgtttc
     3301 cgcgtcactg cgttgcatct gacctaccgt agaggcatcg gtggctgcgc ttccatcacg
     3361 taaaccggaa ataaaacggt taccgatatt catttcccga tcctgttttg cttgcgagtt
     3421 ccatccaaga gcgacagaga gatagccaga agctgaagca tgatcaccca gcgccacaga
     3481 catatgtccg gttgcctttg aatcatgacc aatggcagta ccataagctt ttgcccgtgc
     3541 actctgtccg ataattaatg caccacttgt gttccccagt ggctccaatg gtctcgcgtc
     3601 gtcaccaatg ataatattcc tgctggtaac agagaacgcg tcaccacttc caatgatgac
     3661 gttaccgtaa gaatcgccgc tgttttgagt aggaggagga ttgacagtca caccatcacg
     3721 gataatagtg ttgttatagg ctttataatt caggtaatta ttgttaccta taattctgtt
     3781 gttgttatta tgtatattat ttacaattgt attattattc cctgatatat aattattatt
     3841 tccgtggatg ctgttatttt ctccattgat aatattagta tcttctgcat gagcagtgta
     3901 tacaaaggac agatacatag ttgcaataat accatggaat ttgatgttca tatagtttaa
     3961 aaccttttc
//
LOCUS       DAZGOB010000246         3941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGOB010000246 DAZGOB010000000
VERSION     DAZGOB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.247"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..472
                     /locus_tag="ACHLIF_005033"
     CDS             <1..472
                     /locus_tag="ACHLIF_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9608852.1"
                     /translation="DKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDL
                     VCAQWIWGRIVSLYEQAASYDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFG
                     RLQRDSFWVKNIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            513..935
                     /locus_tag="ACHLIF_005034"
     CDS             513..935
                     /locus_tag="ACHLIF_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9608853.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKPKKPSAVDPDLIWSLPDG
                     EIRRYDRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
     gene            932..1186
                     /locus_tag="ACHLIF_005035"
     CDS             932..1186
                     /locus_tag="ACHLIF_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608854.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            1179..1490
                     /locus_tag="ACHLIF_005036"
     CDS             1179..1490
                     /locus_tag="ACHLIF_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608855.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGNGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            1796..2374
                     /locus_tag="ACHLIF_005037"
     CDS             1796..2374
                     /locus_tag="ACHLIF_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HFO9608856.1"
                     /translation="MRDIDVRKAVHAKILRDHHKDPDTLIIDEFTMNLGASRADIAVI
                     NGLIHGYELKSKSDNLLRLPAQVQHYSSVMDKVTLVVSDCHLYDALSIVPSWWGIKQV
                     TQGARQGIHLKTIRTSKLNPQVDKLSLTMLLWKDELLSLLSDVGELQNLKNKPKRVLW
                     SKLANSMDVGELREAVRVKLKARKEWRVAQQP"
     gene            complement(2334..3431)
                     /locus_tag="ACHLIF_005038"
     CDS             complement(2334..3431)
                     /locus_tag="ACHLIF_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFO9608857.1"
                     /translation="MTISYIPILKAKRSELSALSQLSIEKKSKILPLLEIEPVPIDPD
                     SGIALKSYNETLIEFGKKVSKSCSDMQGVYIDGLLIEEHFISPEDHYPIINAVNQVRD
                     MEIRVIPVSSPTRPSNYKRAIDELMQNEICLRLTTLDLVNPQLITHYINHLGIPLSNI
                     DIIIDLRDELTEDKINSGELYTLAMGLINNLAHLNEYRKVILSGGSFPTDLSDISVGL
                     YSQPRIEWILWQSLMNRKELARNVIYSDYGVQHPDFNRLSTRFPSVSASVRYSGDNDF
                     WVFRGRVANRFGYEQYGKHSEDILAHREYSGPTFCAGDRDIEYYANEYQAYKANPSGN
                     YKFGSPEVWRRIGQNHHITKVVEQLATLYGL"
BASE COUNT         1180 a          751 c          913 g         1097 t
ORIGIN      
        1 agataaaaga tcttcttgtc cggacgcttc gcaaccggac ccgcagacgg ctgaacagga
       61 ttttttaacc cgacaccctg acgcggttgt gttcagtgcg aaaaaacgcc agtggggaag
      121 tcaggaagat ttggtgtgcg cacagtggat ctggggacga atcgtgagtc tttacgagca
      181 ggcggccagc tatgatggcg agatcactag accgaaagaa cccaactgga cagcatgggc
      241 caatgacgtt cgcacaatgc ggatgctgga tggcagaact cacagacaaa tttgtgaaat
      301 gtttgggcgt ctccagcggg attcgttctg ggtaaaaaac atcatgagtc cggcaaaact
      361 ccgggaaaaa tgggatgaac tggttatccg cctggggcgt tcgcctgcgc agcgttgcgt
      421 gaatcacatt tctgaaccgg acactgaaat tccgccgggc ttcagggggt aagtgttaat
      481 ttctggtcat gaggtaattt tcaggagggc ttgtggcaaa agtttttaca caagaagagc
      541 gggaaaaaat taaagggcag gttgttgaac tcgtacgcca gagtgggcgc gagacgttac
      601 gacaactgga aactaaaact ggggcaacaa gatatctgat gagcgttctg gccagagagc
      661 tggttgccag tggcgatgta tacaactctg gttacgggtt attcccgtct gaacaggcgc
      721 gtaaggactg gcaaaatgcc cgtaaaaagc tctcaagggc aaagccgaag aaaccatctg
      781 cggttgatcc ggaccttatc tggtcattac cagatggcga aatacgccgc tacgacaggc
      841 gtctgaacat aatctgtctc gagtgccgga agagcgaagt tatgcagcgc gtactggcgt
      901 tttatcaggg gaattttgag gaggtggtgc ggtgagtgaa tcaaaatgcc aggttaatgg
      961 caacaagata gaaccatgtg cagcactggc aaagtccctt gagcatgatg ctgaatacac
     1021 gatgcgaaaa ggtctgctga tatacaaaat ctggaatgag agtttaactc gcggtcctga
     1081 ttttgtgatg ttgcgttccg gtgaattttc taaattacca gttcgggttt cattttgtcc
     1141 gttctgtggt gaaagtctga aaacgtggga gaacagaaat gaatgaaatt aaagaaatac
     1201 cagtagtacg tgatgaatat ggctgctgga cgcatcctga atatgaaaaa ttctgtgacg
     1261 gtcgggaata tatttcaacg gaagagttta acgcctggat ggaggaaaat aatcttcaat
     1321 acgtcctctg cttcagagat gaaggatgtg ctgaccttga tgcgtgtgat gctgatattt
     1381 ctgcatggga accggaacga ccagagggca atggatggtt tattggttca atacatgaca
     1441 ccgaagatgg cccggtttgt gtatggctga gaaataaggc cgaagcataa aggctataaa
     1501 ccgactaaca actaaatact gaagatttaa atcagaaacg atttttatta aacccttaac
     1561 cggagggatt ctgcaccctc agaacatcag gaggccgtct gaaagggcgg aacagataat
     1621 gcttacgttg aaacatttta tcgacatacc aacatggtta gccgtcattg cttttgttaa
     1681 aatacacatc cacttttctg tgcaatgttt aaccactggt catatcaaat ggcattcatg
     1741 cgaaccatga tatagaatca tggcttgaga gagtcgatga aagcgcaact atggtatgag
     1801 agacattgat gtaagaaagg ctgtgcatgc caagattctg agagatcatc ataaagatcc
     1861 tgacacccta atcattgatg agtttacgat gaatctaggg gctagcagag ctgatatagc
     1921 agtgatcaat gggcttatac atggttatga gttgaagagc aagagtgata acttgctcag
     1981 attaccagcg caggtgcaac attactcatc agtgatggat aaagtaactt tggttgtctc
     2041 tgattgccat ctttatgatg ctttaagcat agttccatca tggtggggga taaagcaagt
     2101 tacgcaaggt gcacggcaag gtatccattt aaaaacaatt cgaactagca agttgaatcc
     2161 acaagtggac aaactttcct taacaatgct tctttggaaa gatgaattgc tttccctatt
     2221 aagtgatgta ggggagctac agaatttgaa aaataaacct aaacgcgtct tatggtcaaa
     2281 actcgccaat agtatggatg ttggcgagct tcgtgaagct gttcgagtta aacttaaagc
     2341 ccgtaaagag tggcgagttg ctcaacaacc ttagttatgt gatggttttg cccaatccta
     2401 cgccatacct ctgggctacc aaatttatag ttaccagagg gattggcttt gtaggcttga
     2461 tactcgtttg cataatattc tatgtctcta tctcccgcac agaatgtagg ccctgaatat
     2521 tctcgatgag caagaatatc ctcactatgt ttaccatatt gttcataacc aaagcgatta
     2581 gctactcttc ctcgaaatac ccaaaagtca ttatctccag agtatctgac gctggcagat
     2641 acgctaggga atcgcgtcga aagcctatta aagtcggggt gctgtactcc ataatcacta
     2701 taaatcacat ttctggcaag ttcttttcta ttcattaaac tctgccataa aatccactcg
     2761 attcgaggtt gagaatatag accaacagaa atatcactga gatctgtagg aaatgaaccc
     2821 ccagaaagaa tcacttttct gtattcattg aggtgcgcca gattgtttat caatcccatt
     2881 gccaaagtat atagttcgcc ggaattaatt ttatcctcgg ttaactcatc tcttaagtca
     2941 ataattatat caatatttga taaaggaatt cccagatgat taatgtaatg cgttattaat
     3001 tgtgggttaa ccagatctaa cgtggttaat ctcaagcata tttcattctg cattaattca
     3061 tcaatcgctc ttttatagtt agatgggcga gttggtgaac tgacaggaat aactcttatc
     3121 tccatatctc taacttgatt aaccgcattt attatagggt aatgatcttc aggagaaata
     3181 aaatgctctt caattaataa tccatcaata taaacacctt gcatatctga gcaagatttt
     3241 gagactttct ttccgaactc tataagagtc tcgttataac tctttaaggc aatacctgaa
     3301 tcagggtcaa ttggcactgg ttcaatttcg agtaatggca aaatttttga tttcttttca
     3361 atggatagct gcgataaagc tgataactca gaacgtttcg ctttcagaat aggaatatat
     3421 gaaattgtca tgttaatacc ttatacagaa tcaattaaca attaatggtt cttcggacat
     3481 tgaaatttat atcgcaaaaa ttggcaaact ctcctcgact actcacttgt gggtatcctg
     3541 gcgtaaaagg aatgacttgg caatacccgg tgtgatcata agtcattgaa aatgatcatt
     3601 tttatcagtc tttcttccat gatgaatgct aatgccattt gatttgttga ggtgaaaact
     3661 gttaaaaatc aaaacgatgt aattgaaatg aacgttcggt agcattcacg ctttaaatgt
     3721 ttcttttgtg ctgattggat gaatttgggt cacttctgat gagagatgtt gcaggaaaag
     3781 aagttggcat tgatctattg gatagttaga attgctgcgg gtgcttgagg ctatctgcct
     3841 caggcatgaa caccaaaagg cagatagaga aaagccccag ttaacattac gcgtccggca
     3901 agacgcttaa cattaatctg aggccaattt catgctttgc a
//
LOCUS       DAZGOB010000247         3905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGOB010000247 DAZGOB010000000
VERSION     DAZGOB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.248"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            98..583
                     /locus_tag="ACHLIF_005039"
     CDS             98..583
                     /locus_tag="ACHLIF_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9608858.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            599..1075
                     /gene="radC"
                     /locus_tag="ACHLIF_005040"
     CDS             599..1075
                     /gene="radC"
                     /locus_tag="ACHLIF_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO9608859.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1144..1365
                     /locus_tag="ACHLIF_005041"
     CDS             1144..1365
                     /locus_tag="ACHLIF_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9608860.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1528..1896
                     /locus_tag="ACHLIF_005042"
     CDS             1528..1896
                     /locus_tag="ACHLIF_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9608861.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            1986..2363
                     /locus_tag="ACHLIF_005043"
     CDS             1986..2363
                     /locus_tag="ACHLIF_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9608862.1"
                     /translation="MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAKR"
     gene            2360..2509
                     /locus_tag="ACHLIF_005044"
                     /pseudo
     CDS             2360..2509
                     /locus_tag="ACHLIF_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            2609..2689
                     /locus_tag="ACHLIF_005045"
                     /pseudo
     CDS             2609..2689
                     /locus_tag="ACHLIF_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3505..3876
                     /locus_tag="ACHLIF_005046"
     CDS             3505..3876
                     /locus_tag="ACHLIF_005046"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO9608863.1"
                     /translation="MLFLPPLQLSAPGSIGFKNRRQLAAWLGLVPRQCFSVGKNTLGS
                     ISKRDDVYIRTLLFHGAKTVSNARKGRQVQESWLGRPVSRRNKNVATVALNNKNARVL
                     WVLLTNDKEISPEEVMVAAYM"
BASE COUNT          976 a         1041 c          981 g          907 t
ORIGIN      
        1 attgtgggtg atggcggaaa ccctgctgga gagcctgcgc tgattcagcc attaccctga
       61 aaaccacgca tttatttgtc ctttaaggag tcatttcatg aaaaccattt cacataattc
      121 caccacatct tctgtttccg taaccgctgc atcagggaat gaccagcctc agttggttgc
      181 caccatagtc cctgatgaac agcgcatcag cttctggccg cagcattttg gcctcattcc
      241 acagtgggtg accctggagc cccgtgtttt cggctggatg gaccgtctgt gcgaagacta
      301 ctgcggtggt atctggaatc tgtacactct gaacaacggc ggggcattta tggcccccga
      361 accggatgac gatgatgacg aaacatgggt actgttcaat gccatgaacg gcaaccgcgc
      421 tgaaatgagc ccggaagccg ctggcattgc tgcctgtctg atgacgtaca gtcatcatgc
      481 ctgtcgtacg gagtgttatg ccatgacggt ccattattac cggctgcggg attacgccct
      541 gcagcatccg gaatgcagcg ccattatgcg catcatcgac tgaatggagg cagggacaat
      601 gcagcagctt tcctttctgc ccggagaaat gacgcccggc gagcgcagtc tcattctgcg
      661 ggctctgaaa accctggacc gccatcttca tgaacccggt gtggccttca cctccacccg
      721 tgcggcacgg gaatggctga ttctgaacat ggcgggactg gagcgtgaag aattccgggt
      781 gctgtatctg aataaccaga atcagctgat tgccggtgaa accctcttca ccggcaccat
      841 caaccgcacg gaagtccatc cccgggaagt gattaaacgt gccctgtatc acaatgccgc
      901 agccgtggtg ctggcacaca atcacccgtc cggtgaagtc acccccagta aggcagaccg
      961 ccttatcacg gaacgtctgg tacaggcgct ggccctggtt gatatccggg tgccggacca
     1021 tctgatagtc ggtggcagcc aggttttctc ctttgcggaa cacggtctgc tttaacccgt
     1081 caccgtcaca accacatcac acctgttttc acttttatct tctgtcttca gaggtatcac
     1141 actatgaaaa tcatcacccg tggtgaagcc atgcgtattc accaacaaca tccgacatcc
     1201 cgtctttttc cgttctgtac cggcaaatac cgctggcacg gcagtgctga agcgtatacc
     1261 ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt ttgcagaacg ccgtaaggac
     1321 agttttggtc cgtatgtccg gctgatgagc gtcaccctga actgatgttc cgtcacccgg
     1381 aaaacgagta cgactggcag agctcagacg ccgctgtgga tgaggtcctg ctcatcaacg
     1441 aatacacctt taccgaagcc ggtctgcgcg ccggctgacc cgtcatcgtt aaactatcat
     1501 tcaccgattt tctgtgagga ttttatcgtg tcagacacac tttccgggac aacgcatccc
     1561 gatgacaatg acgaccatcc ctggtgggga ctaccctgca cagtgacgcc ctgtttcggc
     1621 gcccgtctgg tgcaggaggg taaccggttg cattaccttg ccgaccgtgc cggtatcaga
     1681 ggccggttca gagatgcgga tgcgtaccct ctggaccagg cctttccgct gctgatgaaa
     1741 caactgaaat taatgctcac cagtggtgaa ctgaatcccc gccatcagca taccgtgaca
     1801 ctgtatgcga aggggctgac ctgtgaagcc gacaccctcg gctcctgtgg ttacgtttat
     1861 ctggctgttt atccgacacc tgaaacgaaa aagtaaacca ccggaataac cctctactcc
     1921 ggcctggtgt tttcaccacg ccactgtccc attttttatc tctgcatatc aggaaaatct
     1981 tcagtatgaa aacattaccc gacacgcatg tacgggaggt atcgtgctgc ccgtctctcg
     2041 tcaccatctg gcagacactg ctcacccgac tgctggacca gcactacggc cttacgctga
     2101 atgacacacc gttcgctgat gaacgtgtga ttgaacagca tattgaggca ggcatttcac
     2161 tgtgtgacgc ggtgaacttt ctcgttgaaa aatacgcgct ggtacgtacc gaccagtcgg
     2221 gattcagcgc ctgtacccac tctcagttaa taaacagcat cgatatcctc cgggctcgcc
     2281 gggcaacagg cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggga
     2341 agcatccgga ggcgaaacga tgaaactggc cctgacgctg gaagccgaca gcgttaacgt
     2401 gcaggcgctg aacatggggc gcattgtcgt tgacgtcgat ggtgttaatc tcgctgaact
     2461 gattaacaag gtcgctgaaa acggtgattc actccgcgtg gtggatgaac gtgactctac
     2521 cgaaacaccg gcaacttatg ccagccctca ccagttgctg taagacaatg cagtgataca
     2581 gggtatagtg ccattaacca cagaaacagc actgttgtcc tgtattaagc aggcaagaga
     2641 agatgtcctt accctgcgcc atctgcagct tctgcaccag aaccggtgaa tctcactcaa
     2701 caggcttcat ttcccctgac gccagcctga atacagctgg cgttttcatt tataaacaga
     2761 aaggaaaacc gataataatg gaatggacgc ccctaccatg aagcaacggt aggatattac
     2821 tgataaacct tccagtggag gtgcgttatg gccgttttaa cgattagcat tgacctgaca
     2881 aaaaatgaat tccaaatcca cggtctgggt aggaacagaa aaatctaagc tcagaaaacg
     2941 gattaaacgt tccctgatgc ccgtattctt cgcgaatatg tcttcctgtc ggattgggat
     3001 ggaggtctgc gccggagcgc gtcattagtg agaatgctgt ctacttaggg gcataatgtc
     3061 agactaatgc ccgcgatttg taaagccata tgtgaaaaca aacaagaatg atatggctgc
     3121 tgccgaggcc atctgtgaag ccgttacccg acctaatatg cggtttgtca gcatcaagac
     3181 gcctgagcag tagtcattac tgtcttttca tcgtgcccaa acgggatttg ttaaatcaag
     3241 ggctgcgcag ataaaccaga tcagagaatt actggctgaa tttggcatcg ttttcccgcc
     3301 cgacattgta gcattcagtc gtcatgtccc tgccctgcct gaagacgcgg aaaactgttt
     3361 aacaatgccg ttcagacagc tgtcgtccta tctctaatga aaatgtgaaa caactgagcg
     3421 tacacattga aaccatagaa gccgtgagca acgaacacgt cgggaacgac gaactgtgta
     3481 ggaaaattgc caccatttcg gggattgcta ttcttaccac cactgcaatt gtcagcaccg
     3541 ggtagtatcg gcttcaaaaa ccggcgtcag cttgctgcat ggcttggact ggttccccga
     3601 cagtgcttca gcgtaggtaa aaacacgctg gggtctatca gtaaacggga cgatgtctat
     3661 atcagaacgc tactgttcca tggtgcgaag acagtgtcaa acgccagaaa gggcagacag
     3721 gtccaggaaa gttggctggg cagaccagtc tcgcggcgga ataaaaatgt tgccaccgta
     3781 gcgctgaaca acaaaaatgc cagagtgctc tgggtattgc taaccaacga taaagagatt
     3841 tcaccagaag aagtgatggt agctgcatat atgtaaatga aactacttct gtctgttgaa
     3901 aaata
//
LOCUS       DAZGOB010000248         3866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGOB010000248 DAZGOB010000000
VERSION     DAZGOB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.249"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..230
                     /locus_tag="ACHLIF_005047"
     CDS             <1..230
                     /locus_tag="ACHLIF_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
                     /protein_id="HFO9608864.1"
                     /translation="LYPAQEHLLLQCSYDDKYDFNASTHRGIIGESLTRLGAMFSGKE
                     YQILLPGEIHIKESGKREYKGRDSGLFMQPL"
     gene            complement(238..591)
                     /gene="cmi"
                     /locus_tag="ACHLIF_005048"
     CDS             complement(238..591)
                     /gene="cmi"
                     /locus_tag="ACHLIF_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO9608865.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYHCEEGYVEYAND"
     gene            complement(764..1546)
                     /locus_tag="ACHLIF_005049"
     CDS             complement(764..1546)
                     /locus_tag="ACHLIF_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO9608866.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(1548..1961)
                     /locus_tag="ACHLIF_005050"
     CDS             complement(1548..1961)
                     /locus_tag="ACHLIF_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608867.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            2521..2751
                     /gene="vapB"
                     /locus_tag="ACHLIF_005051"
     CDS             2521..2751
                     /gene="vapB"
                     /locus_tag="ACHLIF_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFO9608868.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            2748..3164
                     /locus_tag="ACHLIF_005052"
     CDS             2748..3164
                     /locus_tag="ACHLIF_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO9608869.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(3326..>3866)
                     /locus_tag="ACHLIF_005053"
     CDS             complement(3326..>3866)
                     /locus_tag="ACHLIF_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608870.1"
                     /translation="ICKSITASADCEEIIKLIYLRRYFEIVDERGDAYQLLSNLFHRR
                     VAPLDYREPAAAGSGYPKMAPEKIQQALRDIREYVDSFDYPRLQALVSSPDEIKNLYR
                     RCRNGYEKLQVFRLLELDQDHPVIRKFVNETYHIENEFICQLDPSRFDLIPEYVIMEC
                     DKLIALPPAANQSSVARIA"
BASE COUNT          967 a          998 c          959 g          942 t
ORIGIN      
        1 aattatatcc agcacaggaa catcttcttt tacaatgttc atatgatgat aaatacgatt
       61 ttaacgccag cactcaccgt ggcatcatcg gagagtcgct cacaaggctc ggggcgatgt
      121 tttctggtaa agagtaccag atactgcttc ctggtgaaat tcacattaaa gaaagtggta
      181 agcgagaata taagggccgg gattccggcc tttttatgca gcctttataa cagtgtatta
      241 gtcattcgca tattcaacat atccctcctc acaatggtat atgccataat acattctctt
      301 accctccggg ccttcagctt taaatgggac aagaagtgtt ttttcatctt caacatctgt
      361 taaacttctc tcaatctttt cccatgtaat tttcgggatt tctgtaccaa gaagttgaac
      421 atcgttgtcc cagcgtttca ttctttgcag gtaatcattt tttacaaacg ttctggcctg
      481 ttcatcgctg acctgatagc atgccggacc tttatcttca gaccagaaaa aaacagcagc
      541 aagagcaata atcgttaaaa taaaaataaa tttcatgcta attactttca taaaatctcc
      601 acaacttctg tcgttcattc gatataaaaa aacattacga atatatccgt acaatgtgag
      661 catactgtga agtggcttta acaattatga aataatacaa gagatcacat attgcagtgg
      721 tctctttttt atagaagaaa tcacaattca taattgtgac ttattatgca tgtttgtttt
      781 tcagcattgt gaccgcatcg gactcaggca tcgaaaactg cacccggtgc cgggcagcga
      841 catccagcgc aaacaccttc gtgtagacct ccgttgagct gatggactta tgccccatca
      901 gactctgcag gaccttcagc ggtataccgg catacagcat atgcatcgca taggaatggc
      961 ggaacgtatg tggcgtgacc gggacagaga acgtcacacc atcagcggca gcggcggcaa
     1021 ccgcctcccc aatccaggtc ctgaccgttc tgtccgtcac ttcccagatc cgcgctttct
     1081 ctgtcctgcc tgttcgttta ttacgccgtt ccatggggat tttcaatgtg gccaccatcg
     1141 tctgcagctg gctgacgtac caggagtcgg agagcggaac cagccggtga gtctgctgcc
     1201 cggcgggcgt tcttcctgcc gtcctggcgg ctttttcggt ccgttgtttc agggtcgcaa
     1261 gctgcacaaa cggatacgga ggcgtgagcg aaaaatcccc ccgcgtcagc gccagtgctt
     1321 cattaatgcg cgcaccggtg ttccacagtg tggccagcag catcttgcgg cgcagatccg
     1381 ggacgtaatg gagcagggcg ctcacttcag gggccagcag gtatttgggc agttcatcat
     1441 gaaccatcga catctggcgg agtgccagcg ctgccggata atcgatggca acaggaagct
     1501 gagcggcatc ccgggcatgg tggatcggtg ccggcaggtg ctgcatatca gtgttcactc
     1561 cacggggaaa cgtcgggcag gttttccaca acccggtact ttcccggata acggagctgg
     1621 ttaccgccac gcgtccagac ggtgacaaca aaaccccctc cctggttgtt ttcggggagc
     1681 aggcgatcgc actgctcact cagcatgtgg atcacgaacg gcgcatcgct gccgtcttca
     1741 aatatcagtt ccagcgcatc gcggccatgc agcgttccct tactgatgat gacgtactca
     1801 gccccccgca tctccctgag taaatgtttt gccgcatccg ggacaagcag cctggctgcg
     1861 ccggcattcc agctgaggta gacatatccg gcctgcgcct gtacagactg ccagtaatcc
     1921 gttctggcga cagcctgacc ttcattctca ataaaaaaca tgggtgactc cttttttctt
     1981 ttccgggcag ggacggtcat gcgataacgt catccatcat cactgctctt accgtcacgc
     2041 catgtgataa catcatgccg ttatcatcat caccatgata acaagcatcc atataaccac
     2101 gctgttaaat taccggagga ccatttccga cagatccgat ttttctgtta ttgacgggtt
     2161 ttcggaagtg tatcacgctg aggaaataat catatcgctg ttttccgccc ctgcgcgacg
     2221 catcacaggt cagggagatg acacaacata tagtgtcatc agcagatgaa tccggcacca
     2281 catgttgtga tgacgggagg tggcgttgtg tggggggaga ggaagatcct gaacaacctt
     2341 ttttcggatc tccgataaaa aagaaagaaa tagagatatg ctaaacatat gcctggcatc
     2401 tacatatgct accgctatac atatgccaca catctgccta tactaaacat atgcacacac
     2461 ccggtgcttt gaatattccg gatatgtttc acatatccat atgctcggga gagaaagaac
     2521 atgagaaccg tatcgatatt taaaaatggc aacaaccgcg ccatccgtct gcctcgtgat
     2581 ctggattttg agggggtgag cgagctcgag atcgtccggg aaggggacag catcatcctg
     2641 cgccccgtcc ggccaacctg gggatcattc ctagagtacg aaaaagccga tccagatttt
     2701 atggcggagc gcgaggatgt tgtcagcgac gaagggcgat ttaacctgtg aacaagactt
     2761 atatgcttga tacctgtatc tgctcgttca tcatgcgtga acagccagaa gccgtcctga
     2821 agcgtctgga gcaggcggtg ctacgcggcc accgcatagt ggtctcggcc atcacctact
     2881 ctgaaatgcg cttcggggct accggcccaa aggcctcacc acgtcacgtc caactggtcg
     2941 atgaattctg cgcccgcctc gatgccatcc tgccctggga tcgcgccgca gtagacgcca
     3001 ccacgaagat taaagtggca ctgcggctgg ccgggacgcc gatcggcccg aacgacacgg
     3061 cgattgccgg gcacgccatc gccgccgggg cgatactggt aacgaataat acaagagaat
     3121 ttgagcgggt gcctgacctg gtgctggaag actgggtgaa gtaagacact cttttcaaag
     3181 tcaggagata agggcaccaa gaaacagccc gtatacgggc tgttttaaca gatatctaca
     3241 gtaagcggtc ctaaactgta gggtatcttc gaagatcaga ctatcaggga gctaaagcct
     3301 atcttccggc ggcaaaaaca gtggctcaag cgatacgggc aaccgagctc tggtttgctg
     3361 ccggtggcag ggcgatgagc ttgtcacatt ccataatcac gtactccggg atgagatcga
     3421 atctggacgg atctagttga cagatgaact cattttcaat gtggtacgtt tcgttaacga
     3481 attttcgtat taccggatgg tcctgatcca gttccagtaa acggaatacc tgcagttttt
     3541 catagccgtt acgacagcga cggtacaggt tttttatttc atctgggctg ctgacgagag
     3601 cctgaagtcg cgggtaatca aaactgtcca catactcccg gatatcccgc aaggcctgct
     3661 gaattttctc aggagccatt ttggggtagc ccgagcccgc agcggcaggc tcgcgataat
     3721 ccagcggggc gacgcgtcga tggaatagat tggatagaag ctgataagca tcaccacgtt
     3781 cgtcaacgat ttcaaagtag cgacgaagat agatgagctt aatgatctcc tcacagtctg
     3841 ctgacgcagt gatggatttg cagatc
//
LOCUS       DAZGOB010000249         3783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGOB010000249 DAZGOB010000000
VERSION     DAZGOB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.250"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1386
                     /gene="dcuC"
                     /locus_tag="ACHLIF_005054"
     CDS             1..1386
                     /gene="dcuC"
                     /locus_tag="ACHLIF_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO9608871.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1427..2107)
                     /gene="dpiA"
                     /locus_tag="ACHLIF_005055"
                     /gene_synonym="citB"
     CDS             complement(1427..2107)
                     /gene="dpiA"
                     /locus_tag="ACHLIF_005055"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO9608872.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2076..3734)
                     /gene="dpiB"
                     /locus_tag="ACHLIF_005056"
     CDS             complement(2076..3734)
                     /gene="dpiB"
                     /locus_tag="ACHLIF_005056"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO9608873.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          864 a          996 c          911 g         1012 t
ORIGIN      
        1 atgctgacat tcattgaact ccttattggg gttgtggtta ttgtgggtgt agctcgctac
       61 atcattaaag ggtattccgc cactggtgtg ttatttgtcg gtggcctgtt attgctgatt
      121 atcagtgcca ttatggggca caaagtgtta ccgtccagcc aggcttcaac aggctacagc
      181 gccacggata tcgttgaata cgttaaaata ttactaatga gccgcggcgg cgacctcggc
      241 atgatgatta tgatgctgtg tggatttgcc gcttacatga cccatatcgg cgcgaatgat
      301 atggtggtca agctggcgtc aaaaccattg cagtatatta actcccctta cctgctgatg
      361 attgccgcct attttgtcgc ctgtctgatg tctctggccg tctcttccgc aaccggtctg
      421 ggtgttttgc tgatggcaac cctatttccg gtgatggtaa acgttggtat cagtcgtggc
      481 gcagctgctg ccatttgtgc ctccccggcg gcgattattc tcgcaccgac ttcaggggat
      541 gtggtgctgg cggcgcaagc ttccgaaatg tcgctgattg acttcgcctt caaaacgacg
      601 ctgcctatct caattgctgc aattatcggc atggcgatcg cccacttctt ctggcaacgt
      661 tatctggata aaaaagagca catctctcat gaaatgttag atgtcagtga aatcaccacc
      721 actgctcctg cgttttatgc cattttgccg ttcacgccga tcatcggtgt actgattttt
      781 gacggtaaat ggggtccgca attacacatc atcactattc tggtgatttg tatgctgatt
      841 gcctccattc tggagttcct ccgcagcttt aatacccaga aagttttctc tggtctggaa
      901 gtggcttatc gcggaatggc agatgcgttt gctaacgtgg tgatgctgct ggttgccgct
      961 ggggtattcg ctcaggggct tagcaccatc ggctttattc aaagtctgat ttctatcgct
     1021 acctcgtttg gttcggcgag tatcatcctg atgctggtat tggtgattct gaccatgctg
     1081 gcggcagtca cgaccggttc aggcaatgcg ccgttttatg cgtttgttga gatgatcccg
     1141 aaactggcgc actcttccgg cattaacccg gcgtatttga ctatcccgat gctgcaggcg
     1201 tcaaacctgg gccgtaccct ttcgcccgtt tctggcgtag tcgttgcggt tgccgggatg
     1261 gcgaagatct cgccgtttga agtcgtaaaa cgcacctcgg taccggtgct tgttggtttg
     1321 gtgattgtta tcgttgctac agagctgatg gtgccaggaa cggcagcagc ggtcacaggc
     1381 aagtaaatag taatgccgga ggcaagtttc ctccggcttt atcatgtcac ccactgtggt
     1441 atatgcgttg cggtctgcca actttgccgt gaacaatttc agcaataatc agatggcggc
     1501 tggcgcaata ttcaagataa cgcctggcag tggtgcgact gatggtcagt gcctgcgcca
     1561 ccgtttctgc cgtatgttgc acaccaggct ctttaaacag ttttcgcacc gcgtttagcg
     1621 tcaagggatc aatgccggtc ggcagttcgt ctttaggttc accgcgagca taggcattaa
     1681 acatctcatc aatttgcttc tggctggcgc tatcaatact ttccagcata tgtttacgct
     1741 ggcggaaacg ggttagcgtt tgccccagcc gttcataggc aatgggctta atgagataat
     1801 caaatacacc acaacgtact gcttcagaca ccgtttccat atcgctggct gcagtggtaa
     1861 acaccacgtc gccgggataa tgcgcctgca ccagttcatg caataaatta atccctctgc
     1921 cgtcaggaag atagttatcg agcaagatta gccccggctt aaaacgctcg atcatcattc
     1981 gggcctgcgc caggtttccc gccagtaata tctgactgaa tccgggaatg tgacgaatat
     2041 attccgcatg catctctgcc agcggcgttt cgtcctcaac gatcaatagg gttaatggag
     2101 ctgtcattag gtttcacttt cggaatatag attgaaaaca aggtaccgca gggatcatta
     2161 tcttcgagag tgataacacc accgcagcgc gttacgtagc tggcaatcaa gtacaaccca
     2221 atgccatgtt caccgggctc gtcagcacgc gtactgaccc cctgctcaaa tattttgtct
     2281 cgtagagact ctggaacgcc gcagccctga tcggcgactt caatcaccac atcatcgcct
     2341 tcatcgctga ggaataattc aacgatcttg tttccttcat cgctacgcag gctggcttcg
     2401 aaggcgttat caagtaaatt gcccacaatg gctgcaaact cggtgctatc cagtcctggc
     2461 ggcagttgcg aaagctggct accggggaca atgatcattt ttagccccag ttcccgggcg
     2521 cgctgcactt taccaaaaag cagccccgcc acctggcgat cggcaaacgc ctcacgcagg
     2581 ctgtcaataa gctgttgctg ggcctgagac tccccctgca ccatcgccag cacgcgatca
     2641 tactctttca tctgcaacag accattgagc gtcgacatcc aattgagatg ctcgtgtcgc
     2701 aatgtacgaa ggctctcgac gtattgtttt atttgcgtca gttgcgcatt gagggtggaa
     2761 atttcgtctt tactacgaaa gctgataatg gcccccagca aatcatcacc tgaacgaata
     2821 gcttcccgat tggcaataac gctcagaccg ttaaagttag ccaccacatc ctgacgtttt
     2881 tcatcaatct gttcggtaaa gaaatcggcg ggcctgacca cttcagcaat gggtttaccc
     2941 aaccattgcc gtccgggaga gctcagcccc agcatctttc ttgcgttacg attgatggcg
     3001 gtaatgtaac catgcggatc caccgcaatc agcccttcat aaaccgaact aaacagcgcc
     3061 tcttgctgac ggaccacgcg tgcgatttgc tttggctcca tgcccatcat ttgccgacgg
     3121 atatgcgctg ccaggaacca cgacagcaac atcagaatcc ctaacagcac gacaaacacg
     3181 cctgccatcg gtaataaaaa ctcagcccgc cagctatcga ttttactcac caggtagcca
     3241 atcgacacca cgccgatgac ttttccatcg tcatcaaaga ttggcgtttt ggcgcgcatc
     3301 gccatcccca ttgacccttt cccggtaatg aagtagctct cccctttctc cagcgcgccc
     3361 tgtttggtga actgcatagg ataaccaatt ttctccggat taggatggta aaggcggatc
     3421 gagtgccggt ccccaatcac cacataatca aaatcggtat ctctttgtaa tttgttagcg
     3481 atggtcgcca gccgtttgta gtcacgcgtt ttcaccgcag agatgacact gtcattggag
     3541 gcaataattt tcgcctgatt cattgccatg tcgcgtacat gaagcgttaa atagtcctca
     3601 aaactggccg taaaatattg cgccagggct gcaatgacaa atattgagaa caccagaatc
     3661 agcaaaaaga tacgcagcgg aaatgccagt ctttggaaaa atgcaaactg tttattctcg
     3721 ttaagctgca acattgtttt ccttgcgggt tgtcccgagc ctctttgacg gatgtaatta
     3781 aaa
//
LOCUS       DAZGOB010000250         3782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGOB010000250 DAZGOB010000000
VERSION     DAZGOB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.251"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            109..2388
                     /gene="ydeP"
                     /locus_tag="ACHLIF_005057"
     CDS             109..2388
                     /gene="ydeP"
                     /locus_tag="ACHLIF_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO9608874.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKWMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2636..2833
                     /gene="safA"
                     /locus_tag="ACHLIF_005058"
     CDS             2636..2833
                     /gene="safA"
                     /locus_tag="ACHLIF_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO9608875.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            2908..3669
                     /gene="ydeO"
                     /locus_tag="ACHLIF_005059"
     CDS             2908..3669
                     /gene="ydeO"
                     /locus_tag="ACHLIF_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO9608876.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
BASE COUNT         1052 a          841 c          870 g         1019 t
ORIGIN      
        1 agtagtcccc tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat
       61 catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa
      121 attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca
      181 gtacgtaagc agatggatat acgccaggat gttattgcca tgtttgacat gaataagcca
      241 gagggctttg actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac
      301 atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc
      361 tctttctttg ctgagaatac ggttcaatca ttactttcct ggggagatca cgagcttgag
      421 gctgcgggac gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca
      481 ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc
      541 aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag
      601 ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa
      661 ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa
      721 gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct
      781 cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat
      841 cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg
      901 acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg
      961 gcgttgctca agtggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca
     1021 ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag
     1081 ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt
     1141 cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt
     1201 tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtacagca actggtcaat
     1261 ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt
     1321 cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt
     1381 ctggctcgcc tgggtgagcg ctatggcttc accccacctc atgcacctgg acatgctgca
     1441 attgccagca tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc
     1501 aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat
     1561 ttggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc
     1621 tatatcctgc cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg
     1681 gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc
     1741 ggtgtaatgc tgaaatcaga gtgtgctgtg gtcgcgggaa tcgcccaggc aacactaccc
     1801 cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt
     1861 gaagctgtgc tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt
     1921 cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc
     1981 attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg
     2041 gcgacagtac gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat
     2101 cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc
     2161 cgtgtaaaaa acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc
     2221 tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg
     2281 acctattttc cagaatcgaa tcacatgcta actcttgata accacgatcc attaagtggc
     2341 attcctggtt ataaaagtat tccggttgaa ttagaaccat caaattaatt cgcctcctca
     2401 tttcttctgc tgtcatccgc acagcagaag aatttctcat tgactattat cgcgcaattt
     2461 gctcacattg attaaattaa accacacact ataagatata aacctctgcc tacagctgta
     2521 agaaactccg ctcagtactg aagtgccagt cctatttcct cttttctcca gcctgttata
     2581 ttaagcatac tgattaacga tttttaacgt tatccgctaa ataaacatat ttgaaatgca
     2641 tgcgaccaca gtgaaaaaca aaatcacgca aagagacaac tataaagaaa tcatgtctgt
     2701 aattgtggtt gtcttattac tgacccttac gttgatagcc attttttcgg caattgatca
     2761 gctgggtatt tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag
     2821 tttgcaagac tgaaaataaa cagcaaatta taaatatgaa atgttaaaaa agtatcgata
     2881 aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat atttattcat
     2941 catgccttca acgctaacat tttagataaa gattacgcct tctctgacgg cgagatcctg
     3001 atggtagata acgctgttcg tacgcatttt gaaccttatg agcggcattt taaagagatc
     3061 ggatttactg aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg
     3121 gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa
     3181 atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga acttttgctt
     3241 ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt
     3301 gtgctatccg tttccggaaa agtgagaaat attgtcaaca tgaagctggc gcacccatgg
     3361 aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta
     3421 aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca gcacgcaaaa
     3481 aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt
     3541 acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa gagagtttct
     3601 aaggagtacc gttgtcaaag tcacacgggt atgaatacgg gcaacacgat gagtgcttta
     3661 gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga attatatggg
     3721 gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat aaataagtga
     3781 ca
//
LOCUS       DAZGOB010000251         3560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGOB010000251 DAZGOB010000000
VERSION     DAZGOB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.252"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            129..2339
                     /gene="katP"
                     /locus_tag="ACHLIF_005060"
     CDS             129..2339
                     /gene="katP"
                     /locus_tag="ACHLIF_005060"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFO9608877.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            2383..2772
                     /locus_tag="ACHLIF_005061"
     CDS             2383..2772
                     /locus_tag="ACHLIF_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO9608878.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            2886..3560
                     /locus_tag="ACHLIF_005062"
                     /pseudo
     CDS             2886..3560
                     /locus_tag="ACHLIF_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3166..3276
                     /locus_tag="ACHLIF_005062"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1018 a          717 c          928 g          897 t
ORIGIN      
        1 tgcggcttgg aaccccttag tattttttgt ctgtagtatc tatcccagca ataggtatat
       61 cctgttgcat caataaagtt gacttttgta tacaacatgc gaatttccct taatccggag
      121 ctattcgtat gataaaaaaa actcttcctg ttctgattct tctggcgcta tcggggagct
      181 tttctaccgc tgtagccgct gataaaaaag agactcaaaa tttctactat ccagaaacac
      241 tggatttaac tcctctgaga ttacacagcc ctgaatcaaa tccctggggg gctgattttg
      301 attatgccac cagatttcaa cagctggata tggaggctct gaaaaaagat atcaaagatt
      361 tgctgacaac ttcccaggat tggtggcctg cggattatgg tcattatggt cctttcttta
      421 ttcgtatggc ttggcacggt gccggaacat acaggacata tgatggccgg ggaggcgcca
      481 gtggtggtca gcaacgtttt gaaccgctga acagctggcc ggataacgtt aatctggata
      541 aagcccgtcg attgctgtgg ccagtcaaga aaaaatacgg ctccagtatt tcctggggag
      601 acctgatggt cctgactggt aatgttgccc ttgaatccat gggatttaaa acgctgggat
      661 ttgctggcgg aagagaagat gactgggagt cggacctggt atactggggg cctgacaaca
      721 agcctcttgc agataaccgg gataaaaacg ggaaacttca gaaacctctt gccgccacgc
      781 agatgggact tatttatgtc aatcctgaag gccccggtgg aaaaccagat cctctggctt
      841 ccgcgaaaga tatcagggaa gctttttcac gtatggccat ggatgatgag gagactgtgg
      901 ccctgatcgc gggagggcat acatttggta aagcacatgg tgcagcgtct cctgaaaaat
      961 gtattggcgc agggcctgat ggtgcacctg tggaggagca gggactggga tggaaaaata
     1021 aatgtggtac aggaaacggc aaatatacca tcaccagtgg cctggaagga gcctggtcga
     1081 catcgccaac ccagttcaca atgcagtatc tgaagaattt atataaatat gaatgggagc
     1141 tgcacaagag tcctgccggt gcttatcagt ggaagcctaa aaaagcggca aatatagttc
     1201 aggacgcgca tgatccgtct gtcctgcatc cgttgatgat gtttacgacg gatattgctc
     1261 ttaaagttga tcctgaatat aagaaaataa ccacccgttt cctgaatgat ccaaaagctt
     1321 ttgagcaggc attcgcaaga gcatggttta aactgaccca ccgggatatg ggaccggcag
     1381 cccgatatct tggtaatgaa gttcctgcag aatcatttat ctggcaggat cctcttcctg
     1441 cggcggatta tacaatgatt gatggtaaag acattaagtc gctgaaagag caggttatgg
     1501 atttgggtat ccctgcatct gagctgataa agacagcctg ggcttcagct tccacatttc
     1561 gtgtgactga ttatcgtggg ggaaataatg gtgcccgcat caggttacag cccgaaatta
     1621 actgggaagt taatgagcct gaaaaactga agaaagtact ggcatccctg acctcattac
     1681 agcgtgaatt taacaaaaaa cagtctgacg gaaagaaagt gtcgttggct gatttaattg
     1741 ttctttcggg taatgctgca atcgaagatg cggccagaaa agccggggtg gaacttgaga
     1801 ttccctttac tccgggaaga actgacgcct ctcaggagca gacggatgtt gcctcattca
     1861 gtgtactgga gccgacagca gatggattca gaaattatta ctcaaaaagc agaagtcata
     1921 tatcgccggt tgaaagcctc attgataaag ccagtcagct ggatctcacc gttcctgaaa
     1981 tgacggcatt actgggtggt ctgcgggtaa tggatattaa tacaaataat tcttcgttgg
     2041 gagtgtttac cgatacccct ggtgttctgg ataacaagtt ttttgttaat ctgctggata
     2101 tgtcaacacg atggagtaaa gcagataaag aagatacata caatggattc gatcgtaaaa
     2161 cgggagcatt aaaatggaaa gcatcctctg ttgatttaat cttcagttca aatcctgaat
     2221 tacgtgcggt ggcagaagta tatgcctcgg atgatgcgag aaataagttt attcatgatt
     2281 ttgttaaatc gtggaataaa gttatgaata gcgatcggtt tgatttaaac aataaatgat
     2341 aaaactgatg agggagggca cctccctcat gaaggagaaa ttatgcagaa aagcaataaa
     2401 gttataatca gctgtggtat ggcattatat tttttgtgtg gggggattac agtggcagaa
     2461 gaacttgatg aaagcatgca agttcttgca aggaatatga gtgtacttca gtcatcaatg
     2521 gataaaaagg agcttcttga agctcttgac agaatggatg atgctgtaga tgagtcaatg
     2581 aagcgtcttc ctgagagcat ttcaccggct gatgaagaag ggaagaatga ctatataaaa
     2641 gaacttcaaa agcttgaaaa agagattatg ctggcgaaac aaaaagtctt atcgggacag
     2701 ttatcagata tacccgattc ggtaaacaag atgaataaaa tcagagttga ggggcatgat
     2761 aaattcagat aaatcagttg gttgattcct gcgggacgaa tacatacgtc aggaacataa
     2821 ctttatggca gtgaaccgcc ccgggaatcc tggagactaa acttcctgag aaagaggtaa
     2881 acaggatgac taaaaatact cgtttttccc ccgaagtccg tcaacgggcg attcatatgg
     2941 ttctggaaag tcagggcgaa tatgactcac aatgggcggc aatttgttcc attgcccaaa
     3001 gattggctgt acaccggaga ctctgcgtgt ctggggacgc cagcatgagc gggataccgg
     3061 gagcggtgat ggtgggctca ccagcgctga acgtcagcgt ctgaaagagc tggaacgtga
     3121 aaaccgtgaa ctgcgccgca gtaacgatat ccttcgccag gcttccgctt attttgcgaa
     3181 ggccgagttc gaccgcctct ggaaaaaata atgccactgc tgctgcgtga gcagtacggg
     3241 gtcggaccgg tatgcagtga actgcatatt gccccgtcaa cgtattacca ctgtcagcaa
     3301 cagcggtatc atccttataa acgtagtgcc tgtgcacagc gcgatgactg gctgaagaaa
     3361 gagatactgc gcttatacga tgagaatcat caggtatacg gtggcgtaaa gtctgacgtc
     3421 agttgttaca ggaaggtatc agggtggcca gatgtatagt ggcgcgcctc atggcggtta
     3481 tgggacttgc cggtgttctc cggggtaaaa aggtccgtac taccgtcagc cggaaagccg
     3541 tttccgcagg cgaccgcgta
//
LOCUS       DAZGOB010000252         3344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGOB010000252 DAZGOB010000000
VERSION     DAZGOB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.254"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..647)
                     /locus_tag="ACHLIF_005063"
     CDS             complement(<1..647)
                     /locus_tag="ACHLIF_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO9608879.1"
                     /translation="MIIDAHTHIGDFVKIRMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKY
                     PNVNFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQSIKICGSDKILFG"
     gene            complement(669..2468)
                     /locus_tag="ACHLIF_005064"
     CDS             complement(669..2468)
                     /locus_tag="ACHLIF_005064"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFO9608880.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTEAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPDCEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNCRPEISPEGVEPWGSAPTSFNFMGGDLWGVIDKLDYLEDLGINGIYFC
                     PIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGADSLI
                     WLDVVRNGANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEE
                     CREYLLSIVRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIKPECYILGEIWHEG
                     TPWLRGDQFDSLMNYPLTYGIIDYFALQDTTKQEFMTSVTRSYLCYPKNITEVMFNLL
                     DSHDTARILSVCLNDKRKVKLAYLFMLTQAGSPCIYYGSEIGIDGFKSMTLENNRKCM
                     IWDENKQDLELRQFIRWLIRLRKKHPQWCVASIQWKDVEHPTVIAYQRDNITFFLNNS
                     EDTANFIYDGRSMEISGFSYEIEGLPAADLYDF"
     gene            complement(2488..3321)
                     /locus_tag="ACHLIF_005065"
     CDS             complement(2488..3321)
                     /locus_tag="ACHLIF_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO9608881.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVLFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
BASE COUNT         1101 a          720 c          556 g          967 t
ORIGIN      
        1 ccaaataaaa tcttatcact accacagatt tttatcgact ggtaaaccat atccgcccta
       61 acccacgcag tatcgccata gagattagga agttttgcaa tcaattcgat cgcttcctga
      121 ttatctgtcg cgagccccat atgacacata acaaaattca catttggata tttaagtgcc
      181 atctcataaa ccaactgtgg gctggattca taatcattag aagtatggct aactaccggg
      241 agatcatatt tttgagcaag gcgtatatat tcctgaactt tatctgaatt gaaagccatt
      301 tttgaatgat atgggtgaac tttgatgcca taaatgatat ctcgattgct ggcaatcata
      361 tcttcaaaat cttgatcaca actttccaga cgaggcttaa tccacataaa cgcgccaatt
      421 cgctttgaat gttgacgaac aagtctgagc atacgttcat tattatcatg ctgtgtaact
      481 tgatcttcat ccggaatcgg gttctggtca tggtcaactt ctactgcaga tccacaagaa
      541 cataatgcaa aatcaatatt atacttgtcc agagatgcca gaaaaacatc ttctggcata
      601 cggattttca caaagtcacc aatatgagtg tgtgcatcaa ttatcataac aaacatccta
      661 attgaaattt aaaaatcata aagatcagca gcaggtaaac cttcaatttc ataactgaaa
      721 ccggagatct ccatacttct tccgtcataa ataaagttgg cagtatcttc actattattt
      781 aagaagaaag tgatgttatc gcgttgataa gcgatcaccg taggatgttc aacatctttc
      841 cattgtattg atgccacaca ccattgtgga tgttttttac gtagacgtat taaccaacgt
      901 ataaattgcc gtaattcaag atcttgtttg ttttcatccc atatcataca ttttctatta
      961 ttctcaagcg tcattgattt aaaaccatca attcctattt cgctaccgta gtagatacaa
     1021 ggagagcccg cttgagttaa cataaataaa taggcgagtt taacttttcg tttatcattc
     1081 aaacaaacgc ttaaaatacg cgcggtgtca tgactatcca gaagattaaa cataacttca
     1141 gttatatttt taggataaca aagataagaa cgtgtaacgg atgtcataaa ttcttgtttt
     1201 gttgtatcct gtaacgcaaa ataatcaata atcccatatg ttaagggata attcattaat
     1261 gaatcaaact gatcaccacg caaccagggg gtgccctcat gccatatctc acccaaaata
     1321 taacattcag gtttgatatc tttaatcacc tttctgaaat ctcgccagaa atgatgatcc
     1381 acttcattcg caacatccag acgccaacca tcaatatcaa aattttgcgt ccaatagcga
     1441 actatactaa gtaagtattc acggcactct tcattctcag tatttagctt gggcatttca
     1501 attacattgc caaaggtttc atagttaaag tttttaaaat cccattcact ttttggagta
     1561 tcgggataaa caggaaattt atgaatccag aaccaatcgg cgtaacgtga attggcacca
     1621 ttgcgtacaa cgtcaagcca tattagagag tcagcgccta aatgattgaa tactgcatcc
     1681 aacatgattt ttattccacg cttatgcgct tcttcaatca aagtatggaa agcaatattc
     1741 ccacctaaat gtggatcaac actaaaatga tcgatggtgt catatttatg gttggaagct
     1801 gaggtaaaaa taggacaaaa ataaatccca ttaataccca gatcttccag ataatcgagt
     1861 ttatcaataa ccccccacag atccccaccc ataaagttga atgaagtcgg tgcactgccc
     1921 cacggttcta ccccttctgg ggatatttca ggtctgcagt tacagaaacg gtctggaaaa
     1981 atttggtacc ataccgtatt ttttacccag gagggggtat ttagaacgtc aattttatta
     2041 agataaggga agcaaaaaaa gttatttaag cggcttaact ctcgttcttc gcagtccggt
     2101 gaactgatat caacacacct tttctcacca aaaagaaact tctctccctc tttcccaaaa
     2161 agtataaaac cgtaacggca acggcgtttt tgaggtttga acactgcaaa ccagtgatca
     2221 tgaaattcgg tgacagcttc ttgttccatc tcgatctcgt tgccgccaat ccagccgaac
     2281 gcttctgtcc cggccatgtt accgccgtca agaccacctt cagcccaatt atatggatcg
     2341 cctatccaca gaataacttt atcaacttct cctcttaatg tgcgtaaccg tacatgcaaa
     2401 gtttcgttat cataggcata gctatcttca ctttttgctg agtgttttaa cgaactaact
     2461 gtaatcataa atactaatcc ctaaaaatta ttccttaacg gcaccgctaa caagtccagt
     2521 agttatatgc ttctgtgtga atacaaataa caatgtgata ggtactgcaa ctaaaagcgc
     2581 cccagcagca aaaagcgtat aattctctgc tgaattagaa gcaatccaac taaaaattcc
     2641 aatcgccagg gtcattttat cctcactacg aagcaccaac gttggcaaga tgaagtccat
     2701 ccatggtcca gtgaacaaaa ctaatccaac gaaaacgaga atcggcttag ccaatggcaa
     2761 tattatctga ataaaaatag tcagatgccc tgctccatct attttagcgg cctcatccaa
     2821 agaagttgga atagcatcaa aataaccttt taccaaccac gtcatgaagg gcaatgaacc
     2881 ggttacataa accagaagca acccagtata tgtgtctatc aaattggcct ttgatagcag
     2941 gatataaatt gcagtcattg ataaaaatgc aggaaacatt tgcaatacaa gaatactcat
     3001 taaagtatta cgctttccac gaaatcgata ccttgaaaaa acgtaggcgg tcatcgtcac
     3061 aacgaacagt gaaattaaca tgttagcggt agcaagaatg aaagtgttca aataccactg
     3121 catatatggc gtatccgtga acaacgttat gtagtggtca aatgtaaact cgatgccact
     3181 aaatgttgaa ctaaatagat tattcccttt tttaaaagat gccattatcg tccatatcac
     3241 tggcccaagc acaagaatgg agttgcagat taaaaaaaga taaactaaga gcgttgatat
     3301 tttacgtaaa aatttatcca ttgtcacatc cctacatcat cttt
//
LOCUS       DAZGOB010000253         3320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGOB010000253 DAZGOB010000000
VERSION     DAZGOB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.255"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..276
                     /locus_tag="ACHLIF_005066"
     CDS             <1..276
                     /locus_tag="ACHLIF_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9608882.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
     gene            328..711
                     /gene="gpFI"
                     /locus_tag="ACHLIF_005067"
     CDS             328..711
                     /gene="gpFI"
                     /locus_tag="ACHLIF_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9608883.1"
                     /translation="MATKEENQKHLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            704..1057
                     /locus_tag="ACHLIF_005068"
     CDS             704..1057
                     /locus_tag="ACHLIF_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9608884.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1072..1605
                     /locus_tag="ACHLIF_005069"
     CDS             1072..1605
                     /locus_tag="ACHLIF_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9608885.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            1602..1997
                     /locus_tag="ACHLIF_005070"
     CDS             1602..1997
                     /locus_tag="ACHLIF_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9608886.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTM
                     VQQGYDYRRDDDMALWSSADLTYSITYEM"
     gene            2005..2757
                     /locus_tag="ACHLIF_005071"
     CDS             2005..2757
                     /locus_tag="ACHLIF_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9608887.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWISSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTG
                     VKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            2771..3202
                     /locus_tag="ACHLIF_005072"
     CDS             2771..3202
                     /locus_tag="ACHLIF_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9608888.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTGSPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGPVHVDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            3229..>3320
                     /locus_tag="ACHLIF_005073"
     CDS             3229..>3320
                     /locus_tag="ACHLIF_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9608889.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRMHYF"
BASE COUNT          773 a          764 c         1047 g          736 t
ORIGIN      
        1 gtctggcgtc tgctgaatgg ttttaagctg ttccgcgaaa aactggatac ccgtcgcggc
       61 tcaaattcac aactcgaaac ggcagtgaaa gacctggggg cggtggtgtc tttcaaaggg
      121 tattacggcg atctggccat tgtggtggcc tcttcccgtt atccgaaaca ctggctgacg
      181 gtaggggatc ccgcccgtga atttaccatg acgcagtccg cgccgctgat ggtgttgccg
      241 gacccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcggggg cgaaatgccc
      301 ccgtgtcttt tttcacaggg ggctgatatg gcaacaaaag aagaaaatca gaaacatctt
      361 cgtcaactgg ctggcctgct ggggcgcgag gcggatatgt cggggagtgc tgcggatatt
      421 gcgcaacgtg tgtctgagtg ggaagaggag cttgctgctt cccgggaggg cattatgcct
      481 ggtgatgaga gcgggcctga gcaaaatcac acagacgatg gtgagcagtt gcacaacact
      541 gatgctacgg atgatgttaa agcggtccgt gtgcggaaat gcctgcatgt gatggggtat
      601 tgcccggaga caggccgtcc cgttgaactg acgtaccggg gcatgcgtgt tctggtgcca
      661 tcaccactgg cgacagccat gatacagcac ggaacggctg agcatgcgtg attttcagaa
      721 tgcctttgat gctgcccttg ccggggtgga cagcacgatt gttgaagtga tggggctctg
      781 tgcgcagttc acctcggggg cacagtgtgg cagcgaagtt cagggggttt ttgacgatcc
      841 ggagtcgctg gggtttgccg gtagcggggt ccgtattgaa ggaagctgcc cgtcattatt
      901 tgtacggacg gatacggttc gtgctgtgcg gcgtggtgac acgctgacca ttaatggtga
      961 gacattctgg gtggatcgtg tttctccgga tgacgggggc agttgttatc tctggctcaa
     1021 ccgtgggcaa ccaccggcag ttaaccggcg acgataaacg cagggtgaat tatggcgata
     1081 aaagggcttg atcaggcgat tgaaaatctg agccgggttc gtaaaaacgc cattccggcg
     1141 gcttcagcaa tggccattaa ccgcgtggcc acaacggcga ttaatcagtc ttcgtcacag
     1201 gttgcccggg agaccagggt gagccggaaa ctggtaaagg aacggtccag actgaaacgg
     1261 gccacggtca gaaatccgaa tgccagaatt atcgttaacc gcggtgatct cccggtgatt
     1321 aagctgggga tcaggatgcc ggggcgtcgt ccggacagca tactcaaagc cggtcagcat
     1381 cggtatcagc gggcatttat tcagcgatta aaaaatggtc gctggcatgt catgcagcgt
     1441 gtggccggga aaaaccgtta ccccattgat gtggtgaaaa tcccgatggc ggccccactg
     1501 aaacaggcgt ttgatgagaa tgttgaccgt atccggcgtg aacgcctgcc cggagaactg
     1561 gcatatgcgc tgaaacaaca actgaggatt gcgataaaac gatgaaacat actgatatcc
     1621 gtgctgcagt gctggatgca ctggagctgc atgaacacgg ggcgacgctg tttgatggtc
     1681 gccccgttgt ttttgacgaa gaggattttc ccgcggtcgc ggtttatctg acggatgcag
     1741 agtataccgg tgaagagctg gatgcggata cctggcgggc cacactgcat attgaagttt
     1801 tcctgcctgc tcaggtgccg gattcagagc tggattcgtg gatggagtcc cggatttatc
     1861 cggcgatgtc cgcgatccct gcactggcag ggatgattac cacgatggtt cagcagggct
     1921 atgactatcg tcgtgatgac gatatggcgt tatggagctc tgcagatttg acttattcca
     1981 ttacatacga gatgtgagga cgatatgcca acaccaaatc ccctggcgcc ggtaaaaggt
     2041 gccggtacca ctctgtgggt ttacaccggc aagggtgatg cttatgcaaa cccgttgtca
     2101 gatgatgact ggcagcgact ggctaaggtg aaggatctga cgccgggcga gatgacggca
     2161 gaatcctacg atgataacta cctggatgat gaagacgcgg actggagcgc gaccgggcag
     2221 ggacagaaat ctgcaggtga taccagtttt acactggcct ggaaaccggg agaagaaggt
     2281 cagaaagggc ttataggctg gtttgaaagc ggggatgtgc gggcctataa aatccgtttt
     2341 ccgaatggca cggtggatgt gtttcgtggc tggatcagca gtatcggtaa ggcagtgacg
     2401 gcgaaagaag tgatcacccg aacagtgaaa gtgaccaacg tgggcaaacc ttccgtggcg
     2461 gaagaacgca gcgaaattac gccggccact gcaattaagg tgacaccgac atccggtacc
     2521 gtggaaaaag gaaaaacaac caccctgact gtttcttttg agccggaaag tgcaaccgac
     2581 aagacgttca gagcggtttc cgccgatccg tcaacgggaa ccattgctgt gaaagatatg
     2641 gtgatcactg tgacgggggt taaggctgga aaagtgagta tccccgtgat ttccggtaat
     2701 ggtcagtttg ccacggtagc tgaagtcacc gttactgaag cgggcgctgc agggtaaacg
     2761 gaggtcatac atgtttctga aaacagaaca atttgaatat aacggtgtgt ctgtcacgct
     2821 ttccgaattg tctgcgctgc agcgtattga gcatcttgcc ctcctgaaac ggcgtgcaga
     2881 acaggcagaa tccagcggca acctgcaggt aagcgtggaa gatctcgtca gaaccggcgc
     2941 gtttctggtg gcgatgtccc tgtggcataa ccatccgcag aaaacggggt caccgtcaat
     3001 gaatgaggcc gtgatgcaga ttgagcagga agtcctgacc acctggcctg ctgatgccat
     3061 tgcccgggcg gaagatgtgg tgttgcgtct gtccgggatg agcgggcctg ttcatgtgga
     3121 tacggatatt accgaagtgg cgaaaaataa cgcgctgact gatgatgatt tttctgcggg
     3181 aaagtcttcg acggcgagct gaattttgcc ctcagactgg cgcgtgagat ggggaggcct
     3241 gactggcgcg ccatgcttgc cgggatgaca tccaccgaat atgccgactg gcgacatttt
     3301 taccgcatgc attattttca
//
LOCUS       DAZGOB010000254         3201 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGOB010000254 DAZGOB010000000
VERSION     DAZGOB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.256"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>3201)
                     /locus_tag="ACHLIF_005074"
     CDS             complement(<1..>3201)
                     /locus_tag="ACHLIF_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9608890.1"
                     /translation="DNLKSTQLLSVIDAISEGPIEGPVDGLKSVLLNSTPVLDSEGNT
                     NISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTITSANIDRLRFTFG
                     VQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTTSQYLASVVVDNLPPR
                     PFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALVGVQVDSEQFGSQQVS
                     RNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCLWDMLTHPRYGMGKRL
                     GAADVDKWALYVIGQYCDQSVPDGFGGTELRITCNAYLTTQRKAWDVLSDFCSAMRCM
                     PVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPN
                     NGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFS
                     VGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISL
                     VDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIRENDD
                     GTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLA
                     RWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQLALGRYTLTVRAVNA
                     WGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIAD
                     IRQVETTARYLGTALYWIAASINIKPGHDYYFYICSVNTVGKSAFVEAVGQPSDDASG
                     YLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSA
                     AIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTSDGMQSQVLLAADR
                     IAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKN
                     ADISGSVNANAGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPS
                     GTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDI"
BASE COUNT          625 a         1002 c          828 g          746 t
ORIGIN      
        1 aaatatcagt atgctctctc tcatgtttag cgccgctgaa tgccaccgcc ggaataacaa
       61 tctgccggtc aaacggctga tcgtcataaa ccctgacggt aatggtccct gatggccacc
      121 gttccggtgc acgggagtcc cgggggaaag ctttgcccac tgttttaacg agatcgcctt
      181 caatctggtt cgcggacagt tttcccagaa cccgacagtt ctcgttaatc gtgacattgt
      241 tgagcgtccc ggcgttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct
      301 ttccgtccgg tgtcagggaa aatgccggtg gatttccacc gctggtaatg gtgggggccg
      361 tcaggcgttt caggaacacg tcgttcatga atatctgatc gccctgacca acaaacatcg
      421 gttttgtgtt gccattcgca ggattaacca tcgcaatcct gtccgccgcc agcagcacct
      481 gactctgcat gccgtcagag gtgttctcaa taccggcacc aatacccgcg atataaaggc
      541 gtccgtcctg catctgctgc agcttcacag cccacatgct gttcaggtta ttatttgtat
      601 caacctgaac cttctgtatc tgctgaattg ccgcactctg gtcttccagt ttcttattga
      661 cggtctgcgt gatttcattg ctgacatccg taatggacgt cctgatttca gccaggtcag
      721 gcgcaagctg accgttatca atctgcgtcc acaactcctg agccagatgg gttttcccta
      781 tctcgccttt gaaaaaatcc agatagccgg atgcatcatc actcggctgg ccgacggcct
      841 ccacaaatgc cgatttgcca acggtgttca cactgcagat gtaaaaataa taatcatggc
      901 ccggcttaat attgatactg gcagctatcc agtacagcgc cgtgccaaga tagcgggctg
      961 tggtttcaac ctgcctgata tccgcaatcc gcttttccga gaaccagaat tcaaactgta
     1021 ccgtcggatc ataaaccgca agatgcggcg tggcggttat ctgaaaatag cccggcgtca
     1081 gctcaatccg cgacggcgct gccggtgcgg caatccggaa cgataccgac gccggatcgc
     1141 cctgctgtcc ccacgcattt accgcccgga ctgtcagcgt gtaacgcccc agcgccagtt
     1201 gcctgaagcg gtatgtggtt tccgtcgtcc gggccgtgct gaccagccgc tcactgccgt
     1261 catccgctgc cacggtcagg cgaagaagga agctaacgcc cttcaccacc ttcggcgtgt
     1321 cccagcgcgc cagcacctga tattccccgc tgtctgcggt gacttcggca gtcaggtgct
     1381 gtaccgctgg cggcgtgaca ccattcaccg tgccgctctg gtcgccgtca aagtgcgccc
     1441 cgttatccac gatggcttct ttttccggta catgctgcac ggcagtgatg gcatacgtgc
     1501 cgtcatcgtt ctcacggata ctcacgcagc ggaacaggcg ctggcgcagc gtcggcaact
     1561 tcagccccca tacgctgtat tcagcaacgc cgtcaggaac acggctcact ttcaccttca
     1621 cgccgtcggt gacggactga acctccacgc tgaccggatt gccacttccg tcaaccaggc
     1681 ttatcagcgt ggtaccggag gatggcagcg tgatttcacg gtcgagcgtc agcgtccggg
     1741 tctggctgtt caccgccagc acgcgcccgc cggtgctgat accggcatag tcatcatcgc
     1801 agatttcaat gacatcgccc ggcacatggc gaagcccttc tgcgcccacg ctgaagtcca
     1861 cggtctgcgt ttccagcagt tctgttttaa tcagccacag cccggcgcgg tgtgcctgcc
     1921 cccggctggt acagccaaaa gcatccatct tcgtgacgtt acgaccgtaa cgggcaatgg
     1981 cctgcgtatc ctccacaagc tctgtcgccg tctcccagcc gttgttcggg tcaatccagt
     2041 tcacctcaac ggcattatgg cggtccttca gggcgctgaa gctgtagcgg aacggcgcgc
     2101 catcatccgg catcaccaca ttactgcggt tataggtcca caccttatcc gacggtcggt
     2161 cctgcacgaa cgtcagcgtc tgcccgttcc ataccggcat acagcgcatc gccgagcaga
     2221 aatcactgag cacatcccac gccttgcgct gtgtggtcag gtacgcatta caggtgatgc
     2281 gcagctccgt gccgccaaag ccgtccggca ctgactggtc gcagtactgg ccgatgacat
     2341 acagcgccca tttgtccaca tccgccgcac caagacgttt ccccatgccg tagcgcgggt
     2401 gggtcagcat atcccacaga caccaggcca tgttgttgct gtatgctggt tttaacgttc
     2461 cgtcccagat accgctgtat tgtcgcgtct gcgggttata gttcgacggc acctgcagaa
     2521 tgcgcccgcg aagatgataa ttacgactca cctgctggct gccgaactgc tccgagtcca
     2581 cctgtacgcc gaccagtgcc gtgttcgggt agcactgttt cacatcgatg atttcggtgt
     2641 atgacgacca gagcgttttg ttctgcagct ggtctgtggt gctgtccggc gtcatcctgc
     2701 gcatccggat attaaacggg cgcggaggca ggttatccac caccaccgag gccagatact
     2761 gcgaggtggt tttgccctta atggtgatgt ctttttccgt cacccagcca ccgttacgct
     2821 gtatctgaac cagcaggcgg acttccgacg gattccggtc cccctttgag gtggtttcca
     2881 ccagtgcctg cacaccgaag gtaaagcgca gacggtcgat gtttgccgac gtgatggtgc
     2941 gggtgatcgg cgtgtcatat ttcacttccg tacccagcac cgtctcggag ccggaggatt
     3001 caaaaccttc cggcggtgtc tgctcctgct cacctgcccg gaacaccacc gtgacgccgg
     3061 agatgttggt attcccctca ctgtccagca ccggcgtact gttcagcagc acgcttttta
     3121 atccatccac cggaccttca atcggccctt cgctgatggc atcaatcaca ctcagcagct
     3181 gcgtggattt caggttatct t
//
LOCUS       DAZGOB010000255         3189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGOB010000255 DAZGOB010000000
VERSION     DAZGOB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.257"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>3189
                     /gene="toxB"
                     /locus_tag="ACHLIF_005075"
     CDS             <1..>3189
                     /gene="toxB"
                     /locus_tag="ACHLIF_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFO9608891.1"
                     /translation="TICPSITTIIKKTEYHGHQLTNAMTVASVVKPYDFSNLGAINSI
                     DKSVSDIPALHTIVEQAKYNLLSWNDFYNTHASIWDTIARQHKSTNIEFHPQSLLFDR
                     DSKGKCLGLSLLYLDTGGYGSRYQKLRHNIETASALYQTKYNDNIKLSNSDDFFLRKT
                     QRIITMSNELGNNRLKNSQLEILELKDPVLTEGILYQRRISSLLITTEYHSLALQQVS
                     SFWRVTDPNFGHCDFHSLAQALTFIKNITSNGNFSSLYGSGIIKIYFSESPNNWKYIK
                     LPLIQNNSLSRDIYLTTPEKLSATGDSLNIMGHLVPVSFIYDIGGVFNGNRISESTDI
                     KNKIKNLKINGDILQHYINTHYLSESQTQKIKDIVDFLGIQDNTKRVKLESDIKHISE
                     VQQPLHSIISRQKEHVKNLLSGLLNEFSNKLRQQGLSLKTNVLSVNNFKASKINSDTV
                     EVTVTDLQGRLYSVDIDTRVIGLTFKEGINSLSEALEHMNIDAIMSVIGLVQYTRMIK
                     TNDNISAIDHAGAVSDIKNIVDKFLGGILTFTNNRVYNPGGISGASLEGFISSGLEVC
                     ASRIGGTAGRYLSNVAKVIKLPLLDIGINIWSLYDSSLNHARATTQIEYISTAIDVSF
                     SSINTALSIGAIAYPPLAIAIVPITIFSHEVKNYAVYVNQINERHKLWLEAEKYLDNG
                     SAKVLSINKDTGIIDLSNNQVLGNVHLDMRENPPILHGEKSYNSGKNIGSHPDMTDRE
                     IMESGAYNFACTKLSDAGEPDIFGWGDKDVCNSMELSESQLANGYSNRQWPSQSPVIP
                     EGIYNTVYLGYGETLRANTEVTLSSTGYFYEIARAYTDDELSEPLLTVCNQRSSVIGG
                     KEPLTIIIPALEHGMLGSNLHMIERFKNYNFSISGGKGGIKLMVGGIGDYNIECTPGV
                     RNIISFEQLSRDFNLDLDLSDERKQNFYFHHPSGFYSGKVMSITQKGINSVVGTKQGY
                     DKIRGNNLDNTFSLGNGGGVIYSGGGSNTYFVPATLQHNLHIYISEKSNGNHIILGET
                     HSRLSIECHFSDDKREFIKMDLYNGGDVILES"
BASE COUNT         1129 a          478 c          592 g          990 t
ORIGIN      
        1 gactatatgt cccagtataa caacaataat aaaaaaaaca gaatatcatg gacatcagtt
       61 aacaaatgct atgacagttg cttcagtggt taagccatat gactttagta atcttggtgc
      121 cataaatagt atagataagt ccgtttctga tatacctgca ttgcatacaa ttgttgaaca
      181 agcgaagtat aatttacttt catggaatga tttttataat actcatgcgt caatatggga
      241 tacaatagca aggcagcata aaagtacaaa tatcgaattt cacccacaaa gtcttctttt
      301 tgatagagat tcgaaaggaa agtgccttgg tttgtctctc ctgtatcttg atacaggagg
      361 ttatggaagc agatatcaaa aactgagaca caatattgaa actgccagcg cattgtatca
      421 aacaaaatat aatgacaata ttaaattatc taatagcgat gatttctttt taagaaaaac
      481 acaacgtatc attacaatgt ccaatgagtt aggcaataat agattaaaaa actcacagtt
      541 agaaatccta gaattgaagg accctgtact aactgaaggt atattatacc agcgtcgaat
      601 atcatctctt ttaataacaa cagaatatca ctcactcgcg ttacaacaag tatcttcttt
      661 ttggagagta accgatccta attttgggca ttgtgacttc cattccttag cacaggcttt
      721 aacgtttata aaaaatataa cgagcaatgg taacttttca tctttatatg gaagtggtat
      781 tattaaaatt tactttagtg agtctcctaa taattggaag tatataaaac tccctcttat
      841 tcagaataat tctttgtcaa gggatatata tttgacaact ccagagaaat tatccgcaac
      901 tggagattca ttaaatatta tgggacattt agtgcctgtc agttttattt atgacattgg
      961 aggagtcttt aatggcaaca gaataagtga aagcacagat ataaaaaata aaatcaagaa
     1021 tctgaaaata aatggggata ttttacaaca ttatataaat acgcattatt tgtcagagtc
     1081 gcaaactcag aagataaaag atattgtgga ttttttaggc attcaggaca acacaaaaag
     1141 agttaagctt gaaagtgata ttaaacatat aagtgaagtt caacagcccc ttcattctat
     1201 tatctcacgg cagaaagaac atgtaaaaaa cttactatct ggattactta atgagttttc
     1261 aaataagtta agacagcaag gcctttcact taaaacaaat gttctttcag ttaataactt
     1321 caaagcatca aagataaata gtgatactgt ggaggttaca gtaactgatc tgcaaggaag
     1381 actttatagt gttgatatag atacaagagt tattggctta acttttaaag aggggataaa
     1441 tagtttatct gaagcactcg aacacatgaa tatagatgca attatgtctg ttattggctt
     1501 ggttcaatat acacgaatga taaaaacaaa tgacaatatt agcgcaatag atcatgcagg
     1561 agcagtatca gatattaaaa atattgtaga caaattttta ggcggaattt taacttttac
     1621 taataatcgc gtatataacc ctggtggtat atctggtgcc tcgcttgaag gatttatatc
     1681 ttctggttta gaagtgtgcg cgtcaagaat aggtggcacc gctgggcgtt atcttagcaa
     1741 tgtggcaaaa gtaataaaat tacctttgct agatataggc ataaatattt ggtcattata
     1801 tgactcttca ttaaatcatg ccagagccac cacacaaatt gaatatatat caacagcgat
     1861 agatgtatct ttttcatcta taaatactgc tttatcaata ggcgcaatcg catatccacc
     1921 tttggcaata gctatagttc cgataactat cttttctcat gaagtaaaaa actatgctgt
     1981 ttatgtgaat caaattaatg agcgacataa gttatggctt gaagctgaaa aatatctgga
     2041 caatggaagc gcaaaggttc taagcattaa caaagataca ggcataatcg atttatcaaa
     2101 taatcaggtg ttaggtaatg ttcatttaga tatgagagaa aaccctccga tattacatgg
     2161 tgaaaaatca tataatagcg ggaagaatat tggaagtcat ccagatatga ctgacagaga
     2221 aattatggag tctggagcat acaattttgc atgtacaaaa ctctcagatg caggtgaacc
     2281 cgatattttc ggttggggag ataaggatgt atgtaattca atggaactgt ctgaatctca
     2341 attagctaat gggtattcta accgccaatg gccttcacaa agtccagtta ttcctgaagg
     2401 gatttataat acagtatatc ttgggtatgg agaaacattg cgagctaata cagaagttac
     2461 attgagtagc actggttact tttacgagat tgcaagggca tatactgatg atgaactatc
     2521 tgagccatta ttaacagtat gtaaccaacg tagtagtgta attggtggaa aagaaccatt
     2581 aacaataatc attccagctc ttgaacatgg tatgcttggc tctaatctac atatgattga
     2641 acgttttaaa aactacaatt tttcaatatc aggagggaag ggcgggataa aattaatggt
     2701 tggtggtatt ggtgattaca acatagaatg tacccccggt gttagaaata ttatttcttt
     2761 tgaacaatta tcacgtgact tcaatctgga tttggattta tcagatgaga ggaaacaaaa
     2821 tttttacttt catcatccct ctggttttta ttcaggaaaa gttatgagta taacgcaaaa
     2881 gggcataaat tcggttgttg gtactaaaca gggatatgat aagattcgtg gtaataattt
     2941 agataatacc ttttcccttg gaaatggggg gggggttata tattctggtg gaggaagcaa
     3001 tacatatttt gttcctgcta cactacagca taatttgcat atatatatat cagaaaaatc
     3061 aaatggtaac catattattt taggtgagac acactctcgc ttatctattg aatgccactt
     3121 tagtgatgat aaaagagagt ttataaagat ggacttatat aatggaggtg atgtaatttt
     3181 agaaagtga
//
LOCUS       DAZGOB010000256         3156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGOB010000256 DAZGOB010000000
VERSION     DAZGOB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.258"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            221..868
                     /locus_tag="ACHLIF_005076"
     CDS             221..868
                     /locus_tag="ACHLIF_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO9608892.1"
                     /translation="MPINLTPYLTASGGLGEIPQDTLSGIRTLAFNGGAQVQMGSTIV
                     TIRSVFLGFFMGSVSPEGLSERALRTALNNVNRLERDLNGGLSGRQILASIPSPYASP
                     PRPSVMQTELVLEQIEKCSFNVNSSSLRATEEALTCPITLCIPEHGVIMRNAGDSDVC
                     TLYDKESLKHLVNTSSPHPLSREKITESMIVKENSCYFDFTSGSIKNVRGEITRL"
     gene            complement(1128..1347)
                     /locus_tag="ACHLIF_005077"
                     /pseudo
     CDS             complement(1128..1347)
                     /locus_tag="ACHLIF_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1562..2884)
                     /gene="nleA"
                     /locus_tag="ACHLIF_005078"
     CDS             complement(1562..2884)
                     /gene="nleA"
                     /locus_tag="ACHLIF_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO9608893.1"
                     /translation="MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVV
                     HIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     SQMALEIPSTVPGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGINFKDLPGPL
                     KNALSNSNIPTTVSTAASTIASATTSTVTTASKDPIPWFGLTAQVVRNHGVELPIVKT
                     ENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDLD
                     NPNTKYAVLTNAAANALGAVGGFAVSRFTGTDPMLSPHIGAMVGQAAGHAIQYNTPGL
                     KPDTILWWAGTTLGLADLNKAETGEGRFTDYPRIWWHAREGAIFPNKEDIARATGADI
                     RAMEEGIPVGQRHPNPEDVVIDIESNGSPHHNPSNYVDTFDIIQETRV"
BASE COUNT          890 a          574 c          675 g         1017 t
ORIGIN      
        1 ggatgaagta acccgacagc ttcaaaaaac cggagtctgg ctccggtttt ttgttgtcat
       61 gtcatggtga tgtttgttaa tgaagttaaa tttattttgg cttgataatg gagtcttatc
      121 ttttgcaaga ctgataatgg tgtgtgaaag cattgtgata agtaagatgc ttcaacagga
      181 aatcaagtaa aatgttaatg aagttaatgg gggttttatt atgccaataa atttaacacc
      241 atatctaaca gcgagtgggg gactggggga aataccgcag gatactcttt ctggcatacg
      301 aacactggct tttaacggag gagctcaggt tcagatgggg agcactattg ttacaatacg
      361 ttctgtcttt ttaggcttct ttatgggaag tgtaagccct gaaggattat cagagagagc
      421 tttacgaact gcattgaata atgttaatcg tcttgaacgc gaccttaatg gagggctatc
      481 cggccgccag attttagcaa gtatacccag cccgtatgct tctccacctc gtccttctgt
      541 aatgcaaaca gagttggtgt tagagcaaat agaaaaatgc tcatttaatg ttaactcgtc
      601 atctctgagg gcgactgaag aggcattaac atgcccgatt acattatgca tccctgagca
      661 tggtgtgatt atgagaaatg ctggagattc agatgtatgt actttatatg ataaagaatc
      721 attaaaacat cttgttaaca caagttcacc tcatcctctg agcagagaaa aaataacaga
      781 gtcaatgatt gttaaggaaa actcgtgcta ttttgatttc acatcaggaa gtattaaaaa
      841 tgttcgtggt gagataacta gactataaga tgttagaaca tattgactga taagtgtaat
      901 tcacagacat cttttcagtt ttttctgtta attttctaat tgatggactg tatacaggga
      961 taaaaggaga gggtattgtc tggacatttc tgttagcata attggaaatg tgcgtgaaaa
     1021 aaagcataac agacatcatg tgttatgtat gtattcagag tgcccataat tcaccagtgc
     1081 agggataaat tgtaaaaaaa tcgcagtacg tcgtatgcag aacggtgcgg cgtacgcagt
     1141 tccagtggac cgtcgcctgt gataacggtc tttgtggaat aaccgttgcg ggagttagct
     1201 cctgatctgg actgattttt ctcattccca agatggtgtg tcatctctgc attgagagcg
     1261 gcttcaacgc tgagcttttt cagcaaccga tcaaactgac tgaggtcttc tgagggggct
     1321 gaggtttttg gccagttcgt tagcaataca ccagaaaaat tctaaacgag cctgtacata
     1381 gatttgtgta attgcctgat tttgatatgt tcaacccaac atcaaatgaa ggttaattta
     1441 tggatgaaaa acagttacag gctatttgtt ttgtgggatt tttaggaaaa agatatatta
     1501 atccttatta ataatagctg tcatccattt cagctattat ttttaaataa ataagtaaag
     1561 cttagactct tgtttcttgg attatatcaa aggtatcaac ataatttgat gggttatgat
     1621 gtggtgagcc attgctttcg atatcgatga ccacatcctc tggatttgga tgtcgctgtc
     1681 caacgggtat accttcttcc atagctcgta tatcagcccc tgttgcacgg gcaatatctt
     1741 ctttattagg gaagatggca ccttctcttg cgtgccacca tatacgagga tagtcagtga
     1801 atctgccttc acctgtctcg gctttgttta aatctgccag ccccagagtc gtacctgccc
     1861 accataaaat agtgtctggc tttaatccgg gggtattata ctgtatggca tgccccgctg
     1921 cttgcccaac cattgcaccg atatgaggac ttaacattgg atctgtacca gtaaatctgg
     1981 atactgcaaa tccccctaca gcaccaagcg catttgcggc agcattggta agaacggcat
     2041 atttagtatt agggttatct aaatcccact tgaaatgtgc gccaaactcc agccctagag
     2101 ttgcctgtag tggagatgca ccatatttaa aatctgcttc tcccggtctg atcacccact
     2161 cttcagtata atttgctttg gggccatcag gagtaagagg agtttctcca acaagcttcc
     2221 atccattttc agttttgact ataggaagtt ccacaccatg attacgaact acttgagctg
     2281 ttaatccaaa ccatggtatt gggtcttttg acgcggtggt taccgtcgaa gtagtggctg
     2341 atgcaatagt ggatgcagca gtcgatacag tggttggtat attgctgttg cttaatgcat
     2401 tttttaatgg accaggaagg tctttaaaat taatacctat ttcatcatgc aaagggaata
     2461 tctgtaaaaa ataattacct gatgtaggat gaatatctgg ttttacaatg tttagctgga
     2521 caccatattt accagagata cccggaacgg tgctgggtat ttctaatgcc atctgagaga
     2581 cattgaataa accaaacata tccaatgctt tttttcctgc agagccttct tcaagatgag
     2641 gtgcaagata tttatccagc ccagagagca gaccttcatc ttttatcaag ctcagtagag
     2701 ctgttgtttc accgcattcc ggtgcatgtc gctgcatatc ctctgggata tgaacaacaa
     2761 atcccgtttc gcatccatta ggtaattcgg attgcggtat ttgtgtaggg gacgtttgtt
     2821 ctgcgtgatg atgtcgattg ttttgtgtgg tgattccgga tgttacgatc ggttgaatgt
     2881 tcatgataaa tccttattgc atgtttatat ccattaataa tatcaatcca ttgatattat
     2941 tagtgtcaca tatccgatgt ggacagcttt aatatatagc tgtttgaatg cttgattgat
     3001 gtgacttaga tctcaaaatt aagttgttaa atttaccagt ttatcgcttg aacacttaaa
     3061 ataaattata aatccttctg taattgtttt tataattcaa attattgttg gttgtataac
     3121 ttgattacat ttttactcta agtgttataa atattt
//
LOCUS       DAZGOB010000257         3144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGOB010000257 DAZGOB010000000
VERSION     DAZGOB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.259"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..87
                     /locus_tag="ACHLIF_005079"
     CDS             <1..87
                     /locus_tag="ACHLIF_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608894.1"
                     /translation="PEGADPNYCWREVDPVDAANEAMSYHND"
     gene            120..337
                     /locus_tag="ACHLIF_005080"
                     /pseudo
     CDS             120..337
                     /locus_tag="ACHLIF_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
     gene            339..602
                     /locus_tag="ACHLIF_005081"
     CDS             339..602
                     /locus_tag="ACHLIF_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608895.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            613..738
                     /locus_tag="ACHLIF_005082"
                     /pseudo
     CDS             613..738
                     /locus_tag="ACHLIF_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1294..1482
                     /locus_tag="ACHLIF_005083"
     CDS             1294..1482
                     /locus_tag="ACHLIF_005083"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9608896.1"
                     /translation="MPDTKTAVLVARDFGRKGDWRMKWATYIPGHPDANDGWIIPGAS
                     WIPSHWMPLPEPPQEVRQ"
     gene            1598..1702
                     /locus_tag="ACHLIF_005084"
     CDS             1598..1702
                     /locus_tag="ACHLIF_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608897.1"
                     /translation="MSDDISLAMEGALAVVAVVGVYCLVVFLMDRLGN"
     gene            1949..2104
                     /gene="hokD"
                     /locus_tag="ACHLIF_005085"
     CDS             1949..2104
                     /gene="hokD"
                     /locus_tag="ACHLIF_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO9608898.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2272..2550
                     /locus_tag="ACHLIF_005086"
     CDS             2272..2550
                     /locus_tag="ACHLIF_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608899.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSRTFKSDWFQHPPCTEEQAEW
                     LIQCYRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            2552..>3144
                     /locus_tag="ACHLIF_005087"
     CDS             2552..>3144
                     /locus_tag="ACHLIF_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9608900.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEHLAQQNLAAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRS"
BASE COUNT          830 a          731 c          836 g          747 t
ORIGIN      
        1 cctgaaggag ccgacccaaa ttattgctgg agggaggttg atccagttga tgctgcaaat
       61 gaagctatga gttatcacaa cgattaatgt caggaggccg cccgaaaggg cggtaatgaa
      121 tggtcacatt atttagaaaa aatatccgcg aaagagtaga acaacagaat ttctgtttct
      181 cattctgttt atcgtgttga tgataccgat atccccgtta atcctagtct ggataatcgg
      241 aaaaataatt gagccagtta ttgaattgta taacgacgtg gtatgggcgt cattcaacac
      301 actgcacaat aaaattaatc cgtataagga aaactgatat ggcaactttg acaaaaaaag
      361 aacgggcatg gttgaacgaa ttacaggaag ttcttgatcg ctgtccatca ccgaaaaaaa
      421 ttggctttta caccattggc gataaaagca tttacctgta tgacctacgc cgcatggatg
      481 aaatcatgga ggctcttgat aatcgttcgt cgatggattg gtgtgttgct gttcatgata
      541 tgaatgcagg gtttgatgaa aagattttgt tcccctcatc agttgaaagc actgcgggtt
      601 aaggagtaac acatgaccac tattaccaaa gaacgtattg aattgttcat taaaaatccg
      661 cttgaaaacg ggcttacccg tggtgaacaa atggaactgg cacggattgc gctggcatcg
      721 ctggaagcag agccggtggc aaagattata gctcattacc cattaggggt tgacgtaggc
      781 aaacaaaaat ttgtacaggc cattagagag cttcctgact ttggcggata tctatttgcc
      841 gcccctccag cgccgatagt gccggaagaa atgtattggc aggatgcgcc agttgaaggc
      901 agcagcaaag cggctgcata cgctacaggc tggaacgatt gccgcgaagc catgtttcag
      961 tccggaaact ttcgggaaaa taaagattcg tcaaccaata attttcggaa aatcccggaa
     1021 gcgtcaacca gctctccggt aactccggct cttctgcctg gtggtttcac cattgaggag
     1081 gcgaaggaat tacatgaaga cctggcacgc agccacataa gcaaggcctt aagtggcgaa
     1141 aagatgaaaa agaaagatcg cgatgctgat ttgcgctgga ttcatggcgt tatagttcag
     1201 gcagcgtggt ttgtaaaagc atcactggag cagaatgcac tatcgggcaa ctatccggta
     1261 actccggata gttggataag ctgtagtgag cgaatgccgg ataccaaaac agccgttctt
     1321 gttgccaggg attttggcag gaaaggtgac tggcgaatga aatgggcgac ttacatcccg
     1381 gggcatcctg acgctaatga tgggtggata atacctggtg cgtcgtggat accatcacac
     1441 tggatgcctc taccagaacc gccgcaggag gtgcgccaat gaactggcct gaagcatttg
     1501 caattacagg cgttgctatg gctatcgctt ttttagtata tgttatttgt cggtgagggt
     1561 aaaaacgttc gccgggatta acaccaaagg agggaatatg tcggatgata tatcactggc
     1621 aatggaaggt gcgctggctg ttgttgctgt tgtgggcgtt tactgcctgg ttgtgttttt
     1681 gatggatcga ctagggaact gaattcatta cgatatggga attcccatat cgggtaaaaa
     1741 cggtttgcgg gaaaaggaga gttaagtaga attgctgcgg gtgcttgagg ctatctgtct
     1801 caggcatgaa caccaaaagg cagatagaga aaagccccag ttaacattac gcgtcctgca
     1861 agacgcttaa cattaatctg aggcccaatc tatgtctcac aaatgtaggt tagcctctta
     1921 cgtgccgaaa ggcaaggaga agcaggctat gaagcagcaa aaggcgatgt taatcgccct
     1981 gatcgtcatc tgtttaaccg tcatagtgac ggcactggta acgaggaaag acctctgcga
     2041 ggtacgaatc cgaaccggcc agacggaggt cgctgtcttc acagcttacg aacctgagga
     2101 gtaagagacc cggcggggga gaaatccctc gccaccgctg atgtgtcagg catcctcaac
     2161 gcacccgcac ttaacccgct tcggcgggtt ttgttttttc tggtcgttct ggtttacaat
     2221 ccatccgtca gcctgaacaa ctggcacctg ctgcgccagc agagaaaaca gatggcgcac
     2281 gataccaaat tttacaattc ggataactct gccgcccctg ccagcaggca cgggcggcgt
     2341 tcccgcactt tcaaatctga ctggttccag caccccccat gcactgaaga acaggccgag
     2401 tggctaattc agtgctaccg cagacacgga tacgagatta agaaagccct cagcctcgat
     2461 tatcgtcact ggataatctc cgtcaggctt ccttactccg aacgaccacc gcgtccgtcc
     2521 cgcacattcc agcaacgcat ctggaggtaa cgtgcgggta ttacttcgac ctgttctggt
     2581 accggaactc gggctggtga tcgttaagcc gggccgtgaa tccatgccgg tattccacaa
     2641 tacccgggta ctggtggagc cggaaccgaa aagcatgcgt aatctgccgt ccggggtcgt
     2701 tcctgccgtt cgccagccgc tggcggagga taaatcatta ctgccatttt tcagcgacga
     2761 acgagtgatt cgtgctgctg gtggcgctgg cgcattgtct gactggttac tgcgccatgt
     2821 taaatcctgc cagtggccac acggtgatta tcaccacagt gaaaccgtca ttcaccgtta
     2881 tggtaccggc gcaatggtgt tgtgctggca ctgcgacaac cagctgcgcg accagacctc
     2941 cgaatcactg gaacatctgg ctcaacaaaa cctggcagcc tggatgattg acgtcatacg
     3001 ccatgcaatg aatggctcgc aggagcgtga attatctctg gctgaattat cctggtgggc
     3061 ggtccgcaat caggtggcgg acgcgctacc ggaagcggta ttacgtcgtt cgctggggtt
     3121 gcgtgcggaa aaaatccgct caat
//
LOCUS       DAZGOB010000258         3133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGOB010000258 DAZGOB010000000
VERSION     DAZGOB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.260"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(97..1956)
                     /gene="gss"
                     /locus_tag="ACHLIF_005088"
     CDS             complement(97..1956)
                     /gene="gss"
                     /locus_tag="ACHLIF_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO9608901.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQEGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNINLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2161..3027
                     /gene="yghU"
                     /locus_tag="ACHLIF_005089"
     CDS             2161..3027
                     /gene="yghU"
                     /locus_tag="ACHLIF_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO9608902.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          746 a          833 c          784 g          770 t
ORIGIN      
        1 aatgaataaa tattggaata tataaatatt gaatattttg attaatcccc gtactaatta
       61 ttcttcataa tcagtacggg gttgctacaa catgtattac tttttcacca caattaacgg
      121 ttcaatatca ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc
      181 gtagttgccg ccaacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa
      241 acaccacagt tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag
      301 cacctcttca tgatggctga ccaggttgat attgctacca cagcgaccgg caatcggttt
      361 cactgcgtaa cctgttttca ccaattcatc attaacagtg aaatcggtat ccagcaggta
      421 acgatggtgc gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac
      481 cgtccacagc ggctcaaaga ctagcacttc cgggcgcagc aatacgtcga taagacgcac
      541 ttcgttttgc ggatgaccgg tacggattgg caccgcagca aactcacggt cgctaacttc
      601 acgaatctga tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagccg
      661 tccttcccca tcaatcagtt gcccggcagc atcccagccc agttcatcca gcccgcgcaa
      721 gatacgcgtt tcaaagccct cctggtgcag cgcctgctcc ataaactgcg cgtgatagtt
      781 ttcctcgata tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca
      841 ggcaccagcc agttcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc
      901 ctgctccgcc caacgttcga ggatcaagcc cgcttcggta tgacatgagg cagagtcggc
      961 gttgtattcg taaaccttca ggccacgctc atccatgcag aaatccatac gaccagtgat
     1021 catatggtga cggcgacgct gccaggagag acgcaaacgt ggccagagga ttttcgggat
     1081 gtcgaacagc gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat
     1141 caggtgcagc tcgttggtgg ctttaattag ctcctgctcg gcactctcgg taatggtgta
     1201 gtaatgataa ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgcag
     1261 cggatctttt tcatccagcc atttaccgtc aaactggcct ttgttttcca ggcgcgctcc
     1321 gctgattttc agcagctcgc ctgcaatttc cggctgcggt aagctgtatt cagtatcttc
     1381 cgtctggatc atccagccca gaatggtggt gtcatcaaaa gtgtctttca gggtatagca
     1441 gccgttttcg accaccatct ccagctcacg cgtccactgt tgcccttgcg gcaacgggga
     1501 atgaatcacg ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc
     1561 gacatggcca gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac
     1621 cggcgcacgc ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac
     1681 ttctcgcagg aagcgcagcg agaaaatctc ccacgccata cccacgtcag taaagaccac
     1741 accgtaattc agaaagagaa aacggcgagc aaattcaacg cattgccact tgtggcccat
     1801 atattcgtcg tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag
     1861 agaactgtaa tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc
     1921 gaacggggca tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc
     1981 taagcagttg gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga
     2041 ggtcagacta atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat
     2101 tctgtacagc aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact
     2161 atgacagaca atacttatca gcccgcgaaa gtctggacgt gggataaatc cgctggcggc
     2221 gcgttcgcca atatcaatcg cccggtttct ggtccgacgc atgaaaaaac gctgcccgtt
     2281 ggcaaacacc cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt
     2341 atgcttgagg agctgctggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt
     2401 cgtattggcg atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag
     2461 atcccggcgc tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga atctggttcg
     2521 atcctgcttt atctggcgga gaaatttggc tacttcctgc cgcaggattt ggcaaagcgt
     2581 actgaaacga tgaactggct gttctggtta cagggcgcgg caccgttcct cggcggtggt
     2641 tttggtcact tttaccatta cgcgccgatg aaaattgagt acgccatcaa ccgctttacc
     2701 atggaagcca aacgtctgct cgacgtgctg gataagcaac tggcgcagca taagtttatt
     2761 gcgggcgatg agtacaccat tgcggatatg gcgatttggc cgtggtttgg caatgtggtg
     2821 ttaggcggcg tgtatgatgc cgcagagttt cttgatgcgg gcagttataa gcatgtacaa
     2881 cgctgggcga aagaagtagg cgaacgtccg gcggtgaaac gtgggcgtat tgttaaccgc
     2941 accaacggac cgctgaatga gcagttgcat gagcgccatg acgccagtga tttcgagacg
     3001 aatacggaag ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag ccatccaggc
     3061 cggataaggc gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc gacgctgccg
     3121 cgtcttatca ggc
//
LOCUS       DAZGOB010000259         3112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGOB010000259 DAZGOB010000000
VERSION     DAZGOB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.261"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            67..399
                     /gene="hdeA"
                     /locus_tag="ACHLIF_005090"
     CDS             67..399
                     /gene="hdeA"
                     /locus_tag="ACHLIF_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO9608903.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            515..841
                     /gene="hdeB"
                     /locus_tag="ACHLIF_005091"
     CDS             515..841
                     /gene="hdeB"
                     /locus_tag="ACHLIF_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO9608904.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            905..1552
                     /locus_tag="ACHLIF_005092"
     CDS             905..1552
                     /locus_tag="ACHLIF_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO9608905.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGIAGVKGVSIS"
     gene            complement(1594..2124)
                     /locus_tag="ACHLIF_005093"
     CDS             complement(1594..2124)
                     /locus_tag="ACHLIF_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO9608906.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2280..2846)
                     /gene="slp"
                     /locus_tag="ACHLIF_005094"
     CDS             complement(2280..2846)
                     /gene="slp"
                     /locus_tag="ACHLIF_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO9608907.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          909 a          608 c          674 g          921 t
ORIGIN      
        1 ggttataacc tcagtgtcaa aattgattcg tgacggctct ttcactttat agttgaggat
       61 attacgatga aaaaagtatt aggcgttatt cttggtggtc tgcttcttct gccagttgtg
      121 agcaatgcag cggatgcgca aaaagcagct gataacaaaa aaccggtcaa ctcctggacc
      181 tgtgaagatt tcctggctgt ggacgaatcc ttccagccaa ctgcagttgg ttttgctgaa
      241 gcgctgaaca acaaagataa accagaagat gcggttttag atgttcaggg tattgcaacc
      301 gtaaccccag ctatcgttca ggcttgtact caggataaac aagccaactt taaagataaa
      361 gttaaaggcg aatgggacaa aattaagaaa gatatgtaat tccgggaatg cgttacatcg
      421 tacttccttg catattgaac aggccggaat atcttcttta aaagcagcta ttcctcctgt
      481 tcatatataa tctctatatt gaatgggtta caaaatgaat atttcatctc tccgtaaagc
      541 gtttattttt atgggcgctg tagcggcttt gtcactggtg aacgcacaat ctgcgctggc
      601 agccaatgaa tccgctaaag atatgacctg ccaggaattt attgatctga atccaaaagc
      661 aatgaccccg gttgcatggt ggatgctgca tgaagaaaca gtatataaag gtggcgatac
      721 cgttacttta aatgaaaccg atctcactca aattcctaaa gtgatcgaat actgtaagaa
      781 aaacccgcag aaaaatttgt ataccttcaa aaatcaagca tctaatgact tgccgaatta
      841 atgaggtaca agtaaaaagg agtagcaagt tgagccatct tgctgctcct ttttgcattt
      901 ttatatgaca gcagaattta ttatacgtct tatactcgcg gcaattgcct gtggcgctat
      961 tggcatggaa aggcaaatgc gcggcaaagg agcagggtta cgcacacatg tattaattgg
     1021 catgggaagc gccctgttta tgattgtttc gaaatatggt tttgctgacg tgctgtcttt
     1081 agatcacgtc ggactcgacc ccagccgtat tgctgctcag gtggtgacgg gcgtcgggtt
     1141 tatcggtgca ggtaacattc tcgttcgtaa ccaaaatatt gtaggtctga cgacggcagc
     1201 ggatatctgg gtgaccgccg ccataggtat ggttattggc agcggtatgt acgaactggg
     1261 tatttatggt tcagtgatga ccttgttggt gctggaagtc ttccatcaat taaccttccg
     1321 cctgatgaac aaaaattacc atttacagct gacattagtg aacggtaata cggtatcaat
     1381 gctcgactgg tttaagcagc aaaaaataaa aacggattta gtttcgttgc aggaaaatga
     1441 agatcatgaa gtggtcgcca ttgatatcca gcttcatgcc acaacgtcga tagaagacct
     1501 gttgcgatta ttaaaaggta tagcaggggt gaaaggcgtt tcaattagct aatctgtgtg
     1561 gcagcgtagc cagactcacc gtaagcctga aattcacacc agataatcaa tatgctgatg
     1621 gcgtaccagc tcattgatcc gcttaagatt gaggatcatc atgatattgt gtttatggca
     1681 gtagaacgtt tttagcgata ttttcagttt ctccagaata tcttcagttg tcatttgatt
     1741 cgcaatgcag ctcagaacct gatgttgctt gcgcgaaagt gacacattcg cttcaggcac
     1801 agagcagtaa ctgccattga tgagtgcgac aaaatcaatg atagtgatag tccgcggaac
     1861 aaaggtcacc ggtactttcc ccagacagcg tttaatatta aagggggaga aaacaatgac
     1921 atggacaggt tttaatcgtt caatttgagt gagaaaatta gaatgaaata cattattcat
     1981 taatgtatca ataatgacga aggcattctg atgcaacatt acgtcgatct cttcttcgtt
     2041 ctgtatatga acgacattac ctttactcag aatgtttttc atcgcggtga agaacatcgt
     2101 atccctggta attataagaa acataaccga ctcctggtgc ggacattaac tttataatca
     2161 gtttgccaaa ggtaattaac aggacagggc ttataataat aatagttagc ttactgtata
     2221 ttaattttat gtaatacatg atgcagatgc tgagaaagca tctgcatctt tcggtggtgt
     2281 tatttgacca gctcaggtgt tacctgactc accgcattgg tgtagtaagg cgcaccccag
     2341 cccggttccg gccagaatgc accatagccg taatcccaca ggttataggt ggtattaacc
     2401 acttctctca aatgccacac ctggatgccc tgcatattca cttccaggaa gttatacggg
     2461 actttattga taaagcctgg ttgttcaccc tgaatggtac cgaggatggt aacaaagtga
     2521 ttacgatagt tcactggatc aaggaagccg ctttgtctgg cgagcagtcg gccctgatag
     2581 ttggcttcaa tatcaggctt cgcatagcta tccaacggta atacagcgat ttctaacaac
     2641 gtatccgttt tgccattgat aacgttgata accttcccac caaagcgcgc ttgttgacca
     2701 acatataacc ccggctggtt atgaacagca acaaaacttt tttgaatatc aggttggtta
     2761 ttgcctttga tattttgcgg aattgaacta catgcggcaa gcaaaaatga aaggctgagg
     2821 atgagtgcac cttttgtcat gttcatgtta ctatccttat caacaaatat taaagccata
     2881 atagatattt ctttatagtt ttcatctgat tctgaggtga tatgcctgat agtaacgtaa
     2941 acctgtctga tgtttatatc ccttttgttt agttcaattc cattgatttt ggggcgtggc
     3001 atgagtatcc atcgccccat aatataatcc tttgttttaa aattacataa aatctatttt
     3061 tattcaataa ttataaagtg ctaataatca atattatggt attatattct ga
//
LOCUS       DAZGOB010000260         3059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGOB010000260 DAZGOB010000000
VERSION     DAZGOB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.262"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            454..1020
                     /locus_tag="ACHLIF_005095"
     CDS             454..1020
                     /locus_tag="ACHLIF_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9608908.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
     gene            995..1597
                     /locus_tag="ACHLIF_005096"
     CDS             995..1597
                     /locus_tag="ACHLIF_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO9608909.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAAEKKLRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCG
                     TLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEA
                     VEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            1594..2259
                     /locus_tag="ACHLIF_005097"
     CDS             1594..2259
                     /locus_tag="ACHLIF_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO9608910.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            2256..2879
                     /locus_tag="ACHLIF_005098"
     CDS             2256..2879
                     /locus_tag="ACHLIF_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9608911.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
BASE COUNT          940 a          608 c          733 g          778 t
ORIGIN      
        1 gtcgtcaata tagtctaact atagttatca ccaaaaactt gcctcgattt tagatttttc
       61 cagtatttgt agatattgca ctgaacaccg aatacgtagc agagggtgtc tacacgataa
      121 cgttctatga gctaccatgt tgtcgaaaaa ttgtttagtg agtatgacat caggaatgtg
      181 gtggtctgtt ttaatatttc tatttctatt tctatttcta tttctatttc tatttctatt
      241 tctatttcta tttctgtttg ttgtggtttt ttctttagtt catgtatttc gatttgtttc
      301 tgaattatcg ggggtagtgt cttccctttt ccctgaggct tatcatgcag ttattttcgc
      361 catcttggta ttgtggaaat accgacatcc atagctttgg tgagctctgt tcaggtggcc
      421 aaattcagaa aaccattacg gaggaagaag gcgatggcta aaccagcgcg aagacgatgt
      481 aaccgtaaaa gagaagattt aactgttaaa aggatatttg agttactaag tttcgataaa
      541 tctaccgggg tatttagatg gaaagttccc actcagggaa ggatagcatt aaatagtgtt
      601 gctggaactt ttgattccaa cggttattca atgatcatga tagatgggcg tagatataaa
      661 actcacgtct tagtttttta cataactcat aatcgttggc ctgctggtca aattgaccac
      721 gttaatggaa ttaggaccga caataggcca gaaaatttaa gagaatgcct gccaatagaa
      781 aattcaagaa atataaggat ccgaaagaat agcaaatcag gttgcagagg ggttacttgg
      841 cacaaacgac agaaaaaatg gaatgttagg ctaggattcc atggcaagag taaacacttc
      901 ggatgctttg atgatctgga gttagcggta ctagttgctg aagaagcccg agataagtat
      961 tacggtgatt tttccggcaa cgaaaggagc acttatgcga atctatcgaa ggaaatgtaa
     1021 atgttgcaat gaatggttta taccaaaata tcaaaatcaa tattggtgta atgagatttg
     1081 tggaaccaag atagcactcg aacgacgaag taaagaacgc gaaaaagcgg aaaaagcagc
     1141 agagaagaaa ctacgacgag aggagcagaa acagaaagat aaactgaaga ttcgaaaact
     1201 cgccttaaag ccccgcagtt actggattaa acaagcccaa caagccgtaa acgccttcat
     1261 cagagaaaga gaccgcgact taccatgtat ctcgtgcgga acgctcacgt ctgctcagtg
     1321 ggatgccgga cattaccgga caactgctgc ggcacctcaa ctccgatttg atgaacgcaa
     1381 tattcacaag caatgcgtgg tgtgcaacca gcacaaaagc ggaaatctcg ttccgtatcg
     1441 cgtcgaactg attagccgca tcgggcagga agcagtagag gaaatcgaat caaaccataa
     1501 ccgctatcgc tggactgtcg aagagtgcag ggccatcaag gcggagtatc aacagaaact
     1561 taaaaaactg cgaaacagca gaagtgaggt tgcatgaata tctacgaaag aattgatggc
     1621 agcaaatacc gaaatatttg ggtagttggc gatctgcacg gatgctacac gaacctgatg
     1681 aaaaaactgg agacgatagg attcgacacc aaaaaagacc tgcttatctc ggtgggcgat
     1741 ttggttgatc gcggtacaga gaacgtagaa tgcctggaat taatcacatt cccctggttc
     1801 agagctgtac gtggaaacca tgagcaaatg atgattgatg gcttatcaga gcgtggaaac
     1861 gtcaatcact ggctgcttaa tggcggtggc tggttcttta atctcgatta cgacaaagaa
     1921 attctggcta aagctcttgc ccataaagca gatgaacttc cgttaatcat cgaactggtg
     1981 agtaaaggaa aaaaatatgt catctgccac gccgattatc cttgtgataa atacgagttt
     2041 ggaaagccag ttgatcatca gcaggtaatc tggaaccgcg aacgaatcag caactcacaa
     2101 gacgggatcg tgaaagaaat caaaggcgcg gacacgttca tctttggtca tacgccagca
     2161 gtgaaaccac tcaaatttgc caaccagatg tatatcgata ctggcgcagt gttctgcgga
     2221 aacctcacat tgattcaggt acagggagaa ggcgcatgag actcgaaagc gtagctaaat
     2281 ttcattcgcc aaaaagcccg atgatgagcg attcaccacg ggctacggct tctgactctc
     2341 tttctggtac tgatgtgatg gctgctatgg ggatggcgca atcacaagcc ggattcggaa
     2401 tggctgcatt ctgcggtaag catgaactca gccagaacga caaacaaaag gctatcaact
     2461 atctgatgca atttgcacac aaggtatcgg ggaaataccg tggtgtggca aagctcgaag
     2521 gaaatactaa ggcaaaggta ctgcaagtgc tcgcaacatt cgcttatgcg gattattgcc
     2581 gtagtgccgc tacgccgggc gcaagatgca gagattgcca cggtacaggc cgtgcggttg
     2641 atatagccaa aacagagcag tgggggatag ttgctgagaa agagtgcgga agatgtaaag
     2701 gcgtcggtta ttcaagaatg ccagcaagcg ccgcatatcg cgctgtgacg atgctaatcc
     2761 caaaccttac ccaacccacc tggtcacgca ctgttaagcc gctgtatgac gctctggttg
     2821 tgcaatgcca caaggaagag tcaatcgcag acaacatttt gaatgcgatc acacgttagc
     2881 gccatgattg ccacggatgg caacatatta acggcataat attgactttt tgaataactt
     2941 tggggaaact tgacaccaat aatgggcgtt ttttacatgt cattgatgag tctcaataac
     3001 ctgccgccga gtagttttta tgctctgaat tgtatttgtg tagtaaacat gctgactgc
//
LOCUS       DAZGOB010000261         2976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGOB010000261 DAZGOB010000000
VERSION     DAZGOB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.263"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..2377)
                     /gene="katE"
                     /locus_tag="ACHLIF_005099"
     CDS             complement(116..2377)
                     /gene="katE"
                     /locus_tag="ACHLIF_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO9608912.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            2560..2823
                     /gene="cedA"
                     /locus_tag="ACHLIF_005100"
     CDS             2560..2823
                     /gene="cedA"
                     /locus_tag="ACHLIF_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO9608913.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT          713 a          724 c          765 g          774 t
ORIGIN      
        1 caatacattg aatttatacg actttgtagg ccggataagg cgttcacgcc gcatccggca
       61 taaacaaagc gcactatgta aatcatttga ggcggcgcaa ttgcgccgcc tcccatcagg
      121 caggaatttt gtcaatctta ggaatgcgtg accacacgcg gtgtgctgcc atcagcgtta
      181 gcagttcatc cataaaacta ccgtcagcgc tgtcagcttc cacaatccct tcttcaccct
      241 ggtcagcgac cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt
      301 taaggtgttt gtaggcttcc atcaggtagt agttggcatc gccgttgtca gcgatatccg
      361 cgatattgcc gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg
      421 tagcggctat aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga
      481 gcagtttggc atgaacgcct ttggccttca gggccttgag aatggccaga aggtctgccg
      541 atctcacttc atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa
      601 tggcgtacaa acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga
      661 tattcagctg gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag
      721 tgagatcaat atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt
      781 tgcttaactc aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta
      841 gccagaacag acgcggatgg gaataatatt cgccaaacga tggactgcgc tcacgaactt
      901 tatttccttc cacgcgttcc tgatatgatt caaaaccgcc gcgtttcggt cccggcggtg
      961 tttcgcgcgg ccagttatcg ttgatcgagt tcggttcgta attcgccggg ttagtgtcga
     1021 tccccatacg atgcatgcca tcacgctgga agttgtggta agggcaggtc ggacggttaa
     1081 tcggaatctc atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga
     1141 acaaacgtcc ctgcaacagc ggatcgttgg tgaagtccag accaggaaca atatgacctg
     1201 gatggaatgc tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca
     1261 ttttgccgac acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa
     1321 gatcgaagtc gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt
     1381 ccggaaaatc gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac
     1441 gtccggtgag tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc
     1501 agtggaaacg tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac
     1561 cgaagccttc catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca
     1621 cgttgtgcag agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt
     1681 gcccttgtgg aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt
     1741 tatgcgcatc ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct
     1801 cttcagtata gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag
     1861 cgccaccctg aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga
     1921 ggaaatccgc tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg
     1981 ctgatccgcg tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt
     2041 tctcgcgcag aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt
     2101 tttgatcatc ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt
     2161 tgcgtacgtc ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc
     2221 caggggcggt aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt
     2281 cctcaggtgc cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact
     2341 ggtgctgatg tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt
     2401 actgaaaggg ccgctgtttt ggtcaaaaaa ccctctaact atagaccatc agccagatcc
     2461 cgcttcggag actattctgg aaacggtgtt atgaaataca cgcaaagtgg gacgtacagg
     2521 ggctaaatcg gctaaactac agtttttctg atttttaata tgcgtttagt gaagccagta
     2581 atgaagaaac cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa
     2641 cccgcgccgc cagaacatgc gataaagatg gattcttttc gtgatgtctg gatgctgcgt
     2701 ggcaaatatg ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact
     2761 gtgcctgaat ccgcccagcg ttgggcaaat cagatccgcc aggaagggga agtgactgag
     2821 taaagattac gtacctcccg gttttgccgg gagttacatg acctgaaatt ctgtatttca
     2881 ttgctacatt cattcaaacc accagtaaca aaaagctgcc tttgacgatc tattgctata
     2941 aataagtgat cttttttctt taattgttta tttatt
//
LOCUS       DAZGOB010000262         2972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGOB010000262 DAZGOB010000000
VERSION     DAZGOB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.264"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..81
                     /locus_tag="ACHLIF_005101"
     tRNA            6..81
                     /locus_tag="ACHLIF_005101"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHLIF_005102"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHLIF_005102"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(300..1058)
                     /gene="yhdZ"
                     /locus_tag="ACHLIF_005103"
     CDS             complement(300..1058)
                     /gene="yhdZ"
                     /locus_tag="ACHLIF_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO9608914.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1066..2169)
                     /gene="yhdY"
                     /locus_tag="ACHLIF_005104"
     CDS             complement(1066..2169)
                     /gene="yhdY"
                     /locus_tag="ACHLIF_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO9608915.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2179..>2972)
                     /locus_tag="ACHLIF_005105"
     CDS             complement(2179..>2972)
                     /locus_tag="ACHLIF_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO9608916.1"
                     /translation="FRNIPPLLQIFFWYFAVLRNLPGPRQAVSAFDLAFLSNRGLYIP
                     SPQLGDGFIAFILAVVMAIILSVGLFRFNKTYQIKTGQLRRTWPIAAVLIIGLPLLAQ
                     WLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYG
                     QHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAAIGYPDMVSLF
                     AGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          808 a          789 c          723 g          652 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcaa aaaggccatc cgaaaggatg gcctttttgc ttttcgaact aacattcaat
      301 taatggatta cctgcgataa aaatgccctc gtacgctctg atttaggatg cgtaaaaaat
      361 tcatcaggtg cagcttgctc cactatttcc ccacgatcca taaaaatcac ccggtcagcg
      421 acggttcgcg caaaccccat ctcatgcgtt acacacaaca ttgtcatacc cgattgcgcc
      481 agcccaatca tcgtatccag cacctctttc accatctccg gatcgagcgc cgacgttggc
      541 tcatcaaaca acataatttt cggcttcata cacagcgaac gcgcaatggc aacgcgttgc
      601 tgctgaccac cagaaatttg tccgggaaac ttatgcgcat gttcggcaat tctcacccgc
      661 tcaaggtaat gcatcgccag agcttcagcc tctttctttg gcatcttgcg tacccaaatc
      721 ggtgccaggg tacagttctg tagaacggtc agatgaggga agagattgaa atgctgaaag
      781 accattccca cttcctgccg gacgcgctca atattgcgga tatcttcatt aagttcgatg
      841 ccatctacca cgatccgtcc ctgttgatgt tcttccagat gattaataca acgaatggtt
      901 gtcgatttac cggaacctga agggccacac agaacgatcc gttctcccgg ttgcacggtt
      961 aaatttatat ttttcaaaac atggaattgt ccataccatt tattgacgtt ttccagcgta
     1021 atcatcgcgt tagcaggttg cagtaaaatt tggctcatag tgtcctcaat gcggtgtacg
     1081 cccggtgtta aaacgttttt ccagatgctg gctatagcgc gacatgctga aacagaagat
     1141 ccagtagatc agcgcggcga aaacataccc ttccgtcgac atacccaacc aggtgggatc
     1201 aacggttgcc tgctgaacgc tactgaaaag atcgaacaac ccgataatga tcaccaggct
     1261 ggtatctttg aagagtgcga tgatggtatt taccagccca ggaattacca gcttcaacgc
     1321 ctgtggcaga ataaccagcc cctgagtttt ccagtaaccc aacgccagcg actctgccgc
     1381 ttcatactgc cctttaggca gtgcctgtaa tccgcctcgc acgacttccg caacatatgc
     1441 tgactgaaac aggatcacgc caaccagcgc gcggatcagt ttgtcgatac tggtgccttc
     1501 tgccataaac aacggcagca tgaccgaaga cataaacaat acggtaatca gcggtacgcc
     1561 gcgccagaac tcgataaaaa tgaccgataa gatacgcaca atcggcatat gggagcggcg
     1621 acctaacgcc agtaagatcc cccacggtag cgcccccgca atcccaacgg atgcaataat
     1681 taaagttagc gtcagcccgc cccattgccg ggtttcaacc cgctccagac caaaaaaacc
     1741 gccatacatc agccaccaga caatcagtgg gtaaatcacc gcccagaccg caatatagcg
     1801 accgcgatgc gggagtattt tccagaacat tggtgcgatt gacacaaggc caatcagtaa
     1861 tgccaggtta attcgccagc gttggtcgtg tgggtaaagt ccatacataa actgaccaaa
     1921 tcgttcgtgg atgaagaccc aacaggcacc ggcttttgtg cagtcggcac gcgttgagcc
     1981 aacccaatta gcctgcaaaa atgcccagtt cagcaacggt gggatcagtt cccacatcaa
     2041 ccatatgcag ccaatagtca gcaggctatt gctccagctg gagaacagat tttttcgcac
     2101 ccacaccatc gcgcggcttg agttatggct cgctgggcgc gagggctgag acagcaatac
     2161 ttttgtcatc atgactcctt agcgttcaac gatcgcgatg cggcggttat agatattcat
     2221 cagcagcgag atagtcaggc tgataatcag atagaccgac atggtcatgg ctatcgtctc
     2281 gatggcttgc cccgtctggt tcagcacggt gccagcaaac agcgaaacca tatcgggata
     2341 gccaatagcg gcggcaagag aggagttttt gacgatgttg agatactggc tggttaacgg
     2401 cggaataatc actcgcagtg cctggggaat aatgacctgg cgtagcgtaa ccgggttggg
     2461 taatcccagt gaccgagccg cttcatgttg accataaggc actgcctgga tcccagcgcg
     2521 gataatctcg gcgataaatg cagatgtata aaccgaaagt gccagcgtta aggctgccag
     2581 ttcaggaatt aaaaccatcc cgccgcggaa gttaaagcct cgtagggctg ggacatccca
     2641 gtgtagtgct gcgccaaaaa gccattgcgc cagtaaaggc aaaccaatga tcaacactgc
     2701 ggcgatcggc caggtgcggc gcagttgtcc ggtctttatc tggtatgttt tattaaagcg
     2761 gaatagccca acagaaagga ttatagccat aacaacagcc aggataaacg caataaatcc
     2821 gtctcccagc tgcggtgacg gaatataaag cccacgattg ctcaaaaagg ccagatcaaa
     2881 cgcgctcaca gcctggcgtg gtccgggcaa attgcgcaac acggcaaagt accagaagaa
     2941 gatttgcagc agcgggggaa tattacggaa ga
//
LOCUS       DAZGOB010000263         2899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGOB010000263 DAZGOB010000000
VERSION     DAZGOB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.265"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..937
                     /locus_tag="ACHLIF_005106"
     CDS             <1..937
                     /locus_tag="ACHLIF_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO9608917.1"
                     /translation="FNWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDF
                     LNGDPDQPIIMGRTYHHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQV
                     YIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQII
                     NVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKV
                     GNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKE
                     QVNGDSLLINWNCAASKSPPKPPDEKQDTPDMREY"
     gene            1005..>2899
                     /locus_tag="ACHLIF_005107"
     CDS             1005..>2899
                     /locus_tag="ACHLIF_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO9608918.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQIRDDALILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGR
                     TLAYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPL
                     SAPSFPDTLPGTEYGADSGIRLSAVWLMHDPEYPENLPAAPLVCYDWTPRGELAAVYD
                     RSGTQMRHFTYDDKYRGRMVGHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSR
                     SGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQ
                     MTAVHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQY
                     DAWGKAV"
BASE COUNT          656 a          788 c          950 g          505 t
ORIGIN      
        1 gttcaactgg gaccgttata acccggcaga ccaagacagt tcgtgctgga tccgtgtggc
       61 acaggcgtgg gcaggcaccg gttttggcca cctggcgata ccgcgtgtgg gtcaggaggt
      121 gattgtggac ttcctcaacg gcgacccgga ccagccgatt attatggggc gtacctacca
      181 ccatgaaaac cgcacacccg gcagcctgcc ggggacgaag acgcagatga cgatccgctc
      241 gaaaacctat aagggcagcg ggtttaatga actgaagttt gacgatgcga cagggaaaga
      301 acaggtctac atccacgcgc agaagaacat gaacaccgag gtgctgaata accgcaccac
      361 ggatgtgata aacaaccatg ctgagaccat tggcaacaat cagatgattg cggttaccaa
      421 caatcagata cagacggtgg gcgttaacca gatagagacg gtgggcagta accagatcat
      481 taacgtgggt tctgttcagg tggaaacgat tggacttgtt cgtgcgctga ccgtgggcgt
      541 ggcgtaccag acgacggtag gtggcattat gaacacttcg gtggctctga tgcagtcctc
      601 gcagatcggt ttgcataaat cgttgagggt cgggctgggt tatgacgtca aagtcggaaa
      661 taacgttacc ttcaccgttg gtaaaacgaa aaaggatgat accgggcaga ccgcgattta
      721 ctccgccggt gagcatctgg agctctgctg tggtaaggca aggctggtgc tgacgaagga
      781 cggacaaatt tttctcaacg gcacaaaaat tcatttgcag ggtaaggagc aggttaatgg
      841 tgactcactg ttgattaact ggaactgtgc agcctcgaaa tccccaccga agccccctga
      901 tgaaaagcag gatacgccgg atatgagaga gtactgatcc cgtacctgtt cggaattcag
      961 taacaaacca gcattaaaaa taaacagaag aaggaatttg aatcatgagc ggaaaaccag
     1021 cggcgcgtca gggagatatg actcagtatg gcggtcccat tgtccagggt tcggcaggtg
     1081 taagaattgg cgcgccaact ggcgtggcgt gctcggtgtg tccgggcggg atgacctcgg
     1141 gcaacccggt gaacccgctg ctcggtgcaa aagtacagcc cggcgagacg gaccttgcgc
     1201 tgcccggccc gctgccgttc attctctccc gcacctacag cagctaccgg acgaagacgc
     1261 ctgcaccggt gggcgttttc ggccccggct ggaaagcgcc ttctgatatc cgcttacaga
     1321 tacgcgatga tgcactgata ctcaacgaca acggcgggcg gagcattcac tttgagccgc
     1381 tgctgccggg ggaggcggtg tacagccgca gtgagtcaat gtggctggtg cgtggcggta
     1441 aggcagcgca gccggacggc cacacgctgg cacggctgtg ggcgtcgctg ccaccggata
     1501 tccggttaag cccgcatctt tacctggcga ccaacagcgc acaggggccg tggtggatac
     1561 tggggtggtc tgagcgggtg ccgggtgctg aggacttact gccagcgccg ctgccgccgt
     1621 accgggtgct taccgggatg gcggaccgct tcgggcggac gctggcgtac cggcgtgagg
     1681 ccgccggtga cctggccggg gaaatcaccg gcgtgacgga cggtgccggg cgggagttcc
     1741 gtctggtact gaccacacag gcacagcggg cggaagaggc ccgtaaaccg cacaccgctt
     1801 cgttatcttc ccctgacagt ccccgccctc tatcagctcc ttccttcccc gacacactgc
     1861 ccggtacgga atacggcgct gacagcggta tccgtctttc ggcggtgtgg ctgatgcacg
     1921 acccggaata cccggaaaat ttacctgccg cgccactggt gtgctacgac tggacgccac
     1981 gcggtgagct ggcggcggtg tatgaccgca gcggtacgca gatgcgccac tttacttacg
     2041 atgataaata ccggggccgg atggtggggc accgttatgc aggcaggccg gagatgcgct
     2101 accgctacga cgatgcgggg cgggtggtgg agcaacttaa cccggcaggc ctgagttacc
     2161 gctaccagta tgagcaggac cgcatcaccg tcacggacag cctgaaccgg cgtgaggtgc
     2221 tgcatacaga aggcggggcc gggctgaagc gggtggtgaa aaaagaactg gcggacggca
     2281 gcgtcacgca cagcgggtat gacgcggcag gaaggctcac ggcgcagacg gacgcggcgg
     2341 gacggcggac agagtacggt ctgaatgtgg tgtccggcga tatcacggac atcaccacgc
     2401 cggacgggcg ggagacgaaa ttttactata acgacgggaa ccagctgacg gcggtggtgt
     2461 ccccggacgg gctggagagc cgccgggaat atgatgaacc gggcaggctg gtatcggaga
     2521 catcgcgcag cggggagaca gtacgctacc gctacgatga cgcgcacagt gagttaccgg
     2581 cgacgacaac ggatgcgacg ggcagcaccc ggcagatgac gtggagccgc tacgggcagt
     2641 tgctggcgtt cactgactgc tcgggctacc agacccgcta tgaatacgac cgcttcggcc
     2701 agatgacggc ggttcaccgc gaggaaggta tcagccttta ccgccactat gacaaccgtg
     2761 gccggttaac ctcggtgaaa gacgcacagg gccgtgaaac gcagtatgag tacaacgccg
     2821 caggcgacct gactgccgtt atcaccccgg acggcaaccg gagcgagaca cagtacgatg
     2881 cgtggggaaa ggcggtcag
//
LOCUS       DAZGOB010000264         2871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGOB010000264 DAZGOB010000000
VERSION     DAZGOB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.266"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..500
                     /locus_tag="ACHLIF_005108"
     CDS             <1..500
                     /locus_tag="ACHLIF_005108"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9608919.1"
                     /translation="FHLMGSHGPTYYLRYPEPHKYFKPTCDRSDIENCTHEQLINTYD
                     NTIRYTDYIISKLIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAEQT
                     HVPLITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDI
                     ISSCR"
     gene            complement(593..952)
                     /locus_tag="ACHLIF_005109"
     CDS             complement(593..952)
                     /locus_tag="ACHLIF_005109"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO9608920.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
     gene            1638..1928
                     /locus_tag="ACHLIF_005110"
     CDS             1638..1928
                     /locus_tag="ACHLIF_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO9608921.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            1952..2203
                     /locus_tag="ACHLIF_005111"
     CDS             1952..2203
                     /locus_tag="ACHLIF_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO9608922.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(2251..>2871)
                     /gene="traT"
                     /locus_tag="ACHLIF_005112"
     CDS             complement(2251..>2871)
                     /gene="traT"
                     /locus_tag="ACHLIF_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO9608923.1"
                     /translation="QYTSTIWLEPASERTVFLQIKNTSDKDMSGLQGKIADAVKARGY
                     QIMTSPDKAYYWIQANVLKADKMDLRESQGWLSRGYEGAAVGAALGGGITAYNTGSAG
                     TTLGVGLATGLIGMAADAMVEDINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKI
                     QTSTETGNQHKYQTRVVSSANQVNLKFEEAKPHLEDQLAKSIANIL"
BASE COUNT          812 a          570 c          601 g          888 t
ORIGIN      
        1 tatttcattt gatgggtagt catggtccta cctactatct ccgttatcct gagccacata
       61 aatattttaa accaacatgt gatcgtagtg atatcgaaaa ttgtacgcat gagcaattga
      121 ttaataccta tgataataca attcgctata ctgattatat aatatcaaaa ttaattgaca
      181 agcttatcga atataaagat gagtatgata ctgttttatt atatgtctct gaccatggag
      241 aatctctggg agaaaatgga ttatacttac atggaacacc atataatgtt gctccagctg
      301 agcaaacgca tgtgcctctt attacctgga tgtctccggg gttcgtttcc tctaaaaaaa
      361 ttgatttgaa ttgtttgtca gaacatgctc taaacagaac agtaagtcat gataatatat
      421 tttcatcgtt actggggtta tggaatgtaa atacttcagt ttataataaa gaagatgata
      481 ttatatcctc ctgcagatag atactgacat ttttctgagg ataagaggtg aatacctctt
      541 atccttaagt aaatattaac caggtaactg tgaataattt agatgttggg cgttatgaaa
      601 gtaataaaac tccccatata atgatagcaa ctaaaatcgt aatcaacact gcggctgaac
      661 ccatatcttt tgctctgcct gataattcgt gatgttctga accaattctg tcaacaactg
      721 cttcaattgc actgttaatt atttcaacaa taattaccat cattgccgaa ccaatgagta
      781 atactcgagt aacagagtca acatccagcc aaaaggatat tatcagtgct gacagaacca
      841 gaatgcactc ctgacgaaag gctgcttcat agcgccatgc cgcacataag cctttccatg
      901 aataacccgc tgctttaata attctggtaa caccagtggt cgtattagcc ataaatacct
      961 cttatttaat tatatgtctg aatatgcatt ataaaatatc agttaacatt aacaggattt
     1021 tgtaataagc cacatttatt ctttcgggaa ttaaaaaaca taacgaaatt ttgcctcatt
     1081 ctgcatttca ttgaaatagt atgtttctca tcgaaagcaa gtttgtcaat ataaatttgt
     1141 aacagaaatg actttccggg gataaacaaa actgttcatg gcaatggctt tctctgtcgc
     1201 caacatggat acattgttcc ggataaagca ctgatgtcat tgactgacgc agactatcag
     1261 gccatctgat gctttctttt ccttcaccgg tattgatgat atgccttatg aagggggact
     1321 tattcctgta acccaatcac aggcactgtg attactggtt tatgattgat gctgatttga
     1381 gcagatatgc acagcagttg tggattcagg ggatattttc tgccagatat tcataccaga
     1441 ttgatgacct ctttgctcag atgctcttcg ttctgagcca gccaggattt tgtggccaca
     1501 tacattgttc gccgatgggg aatcgagaca ggcgattggc tgtgatgcga ttttgccgct
     1561 aactgaggat gagtccgccc ctcccgggga attcctgtaa gttaagccgt acctgttgat
     1621 tagcactaaa ctgggtaatg aatttatctg ccagtatgaa tgatgccggg gacggttttg
     1681 aactcctgat gtcgggtaat atgcaggatt ttaatatttg ttgtcgtcgg gataatcatt
     1741 atcctgaaca taaactgata cctttatcag agttatttga cgttaattat ggactgaatc
     1801 ttgagctgaa taaactggag aaagattctt caggaattaa ttttgtttca agaacgtcca
     1861 aaaataatgg cgtctccgcc agggttaaat taaaagatgg tattagtcca tgccagcagc
     1921 tgtgttaact gtagcagcag gaggttctgt tatggaatcg tttgtacagg attcaccatt
     1981 ttatagtgga agagatcttt attggttacg tcctaaagtt gaactgactc tggaagagaa
     2041 attgtactac tgctcttgta taagaagaaa cagacacaaa tacagctacg ggcgtcaggc
     2101 aaaccgaacg ctgaagaatt tactggtacc gtcattagat tctgttccgg catgggtata
     2161 tggtgtgaca gggaaaataa tatctgaatt gtctgaacgt tgagactaag agggccggag
     2221 gccaggtgaa tggccaccgg gatatttata tcagagtata ttggcgattg attttgccag
     2281 ctggtcttcc agatgaggtt tagcttcttc aaacttcaga ttaacctggt tggcactgga
     2341 tacaacccgg gtctggtatt tatgctggtt gccggtttct gtgcttgtct gaattttgga
     2401 gcctgatgtt ccctgacgca aagctgcaac attatccgtt ctgacgctgg ttctggttcg
     2461 ctcggcaatc tgaacatctg taatcattgt gtaatttata tcttccacca ttgcatccgc
     2521 ggccatgcca atcagaccgg tagccagtcc aacaccgaga gtggtacctg ccgacccggt
     2581 attgtacgct gtgattccac ctccaagagc agctccgact gctgcacctt cataaccgcg
     2641 actcagccat ccctgagatt cacgcagatc cattttgtct gctttgagaa cgttagcctg
     2701 gatccagtag taagctttat ccggggaagt catgatctga taacctctgg ctttgactgc
     2761 atcggcaatt tttccctgta gtccactcat gtctttatcc gacgtgtttt tgatctgcag
     2821 gaaaacggtg cgttcgctcg caggttcaag ccagatggtt gaggtgtact g
//
LOCUS       DAZGOB010000265         2825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGOB010000265 DAZGOB010000000
VERSION     DAZGOB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.267"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1472
                     /gene="escC"
                     /locus_tag="ACHLIF_005113"
     CDS             <1..1472
                     /gene="escC"
                     /locus_tag="ACHLIF_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO9608924.1"
                     /translation="LEKRLGKNEYFIITKSSPVRAILNDFAANYSIPVFISNSVNDDF
                     SGEIKNEKPMKVLEKLSRLYHLTWYYDENILYIYKTNEISRSIITPTYLDIDSLLKYL
                     RDTISVNKNSCNVRKITTFNSIEVRGVPECIKYITSLSESLDKEAQSKAKNKDVVRVF
                     KLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLPSTGKGAVERNGNLFDNSVKI
                     SADPRLNAVVVKDREITMDIYRQLISELDIEQRQIEISVSIIDVDANDLQQLGVNWSG
                     TLNAGQGSIAFNSSTAQANLSSSVISNANNFMIRVNALQQNSKAKILSQPSIITLNNM
                     QAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRILDDNSNSLTGKRRERVRLLLD
                     IQDGNQSTNQSNAQDASSILPEVQNSEMTTEATLSAGESLLLGGFIQDKESSSKDGIP
                     LLSDIPVIGNLFSSTTKQKHSVVRLFLIKATPIKSASSE"
     gene            1486..1941
                     /gene="cesD"
                     /locus_tag="ACHLIF_005114"
     CDS             1486..1941
                     /gene="cesD"
                     /locus_tag="ACHLIF_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO9608925.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
     gene            complement(2327..2740)
                     /gene="grlA"
                     /locus_tag="ACHLIF_005115"
     CDS             complement(2327..2740)
                     /gene="grlA"
                     /locus_tag="ACHLIF_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFO9608926.1"
                     /translation="MGSKNKNGDYVIPDSVKNYDGEPLYILVSLWCKLQEKWISRNDI
                     AEAFGINLRRASFIITYISRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIES
                     PVSTGPKRKNYRVGNGIVGQSNIWNEMIMRRKKEN"
BASE COUNT          944 a          537 c          503 g          841 t
ORIGIN      
        1 ctctggaaaa aagattaggc aagaacgagt attttattat tacaaaaagc tctccggtac
       61 gtgcaatcct caatgatttt gccgctaatt atagcatccc tgttttcatc agtaattctg
      121 tgaatgatga tttctctggt gaaataaaaa atgaaaaacc aatgaaagta ctggaaaaac
      181 tgtccaggct ctatcatctc acatggtatt atgatgagaa cattttgtat atttataaaa
      241 caaatgaaat atcaagaagc ataattacgc caacatatct ggatattgac tcactgttaa
      301 aatatctgcg tgatacaatc tcagtaaata aaaactcctg taacgtgaga aaaatcacaa
      361 catttaactc gattgaagtt cgtggcgttc ctgaatgcat aaaatacatt acatctttat
      421 ctgaaagtct tgataaagaa gcacagtcta aagcaaaaaa taaagatgtc gttagagtat
      481 ttaaattaaa ctatgcatca gcgacagata taacgtataa ataccgcgac caaaatgttg
      541 tcgtgcctgg cgtcgtctcc atcctgaaga cgatggcaag taatggctca cttcccagca
      601 caggcaaagg tgctgtagaa agaaacggca acctgtttga taacagtgta aagatttccg
      661 cagatccacg tcttaatgca gttgttgtaa aagatcgtga aataactatg gacatatatc
      721 ggcaattaat ttctgagctt gatattgagc aaaggcaaat tgagatttct gtttcaatta
      781 ttgatgttga tgcaaatgat cttcagcaac tgggagtaaa ctggtccggt acattaaatg
      841 cagggcaagg ttctattgcc tttaacagct caacggcgca agctaatctc agttcttctg
      901 tgataagcaa cgccaataat ttcatgatca gagtgaatgc gttacaacag aactcgaaag
      961 caaaaatact ctctcagcca tcaattataa ccttaaataa tatgcaggcg attcttgata
     1021 aaaacgtaac gttttataca aaagtttccg gagaaaaggt ggcatctctg gaaagtatta
     1081 ccagcggaac attactgaga gtaacaccac gtatacttga tgacaactcg aatagtctta
     1141 ccggaaaacg tcgggaacga gttcgtttgt tacttgatat tcaggatggc aatcaatcga
     1201 ctaaccaaag taatgctcag gatgccagca gcatcttacc tgaagtacaa aattcagaaa
     1261 tgacaacaga agcgacatta agcgctggag aaagtctgct gcttggcgga tttatccagg
     1321 ataaagagag ttcaagtaaa gatggtatac cgttgttaag tgatattcct gttattggta
     1381 acttattctc cagtacaaca aaacaaaaac acagcgttgt tcgactcttt ttgattaaag
     1441 cgaccccgat aaaatcagca tctagcgaat aataaatgag gctttatgag caggaaattt
     1501 agctctctac aggatatcta tgatttctac caggatggtg gtacgttagc atcactgaca
     1561 aatttgacgc aacaagatct caatgatctt cattcttatg cctatacagc atatcaatct
     1621 ggtgatgtta taaccgcaag aaatctattt catctgctaa catatctgga gcactggaat
     1681 tatgactaca ccctatctct ggggttgtgt catcagcgtt tatcaaatca tgaagatgca
     1741 caactgtgtt tcgctcgctg tgcaacttta gttatgcaag atcccagggc atcttattat
     1801 tctgggatta gctacttatt ggtcggaaat cggaaaatgg ccaaaaaagc ctttaaggct
     1861 tgtttaatgt ggtgtaatga acaagaaaaa tacgctacat ataaagaaaa tattaaaaaa
     1921 ttgttaggta atacagatta aaaactacca tctgccatac caatccccag gctgtctggg
     1981 gattttctat cggaaataat aaagctgctt tggtgaagtg cgcccaacgt tgagcaccga
     2041 ccgctggcag atttatctac aggagtgggt tactccatta accaggattt tatgcgcctg
     2101 aaccatcttc tgctgtactc aatgtcattt ccgcttatag aataagcagt atatatctta
     2161 acttagcatc agaaatctga ctgtttcaat catctatagc ccagagtgta tttgtcagga
     2221 aatgactttc aaactttggc agagaaaaag gcttatcctg gaaaacaaag cccttgaata
     2281 gagctttatt tttattcttc tataaaatat actcaaaaaa atacgtttaa ttctcctttt
     2341 tccgcctcat gatcatttcg ttccagatat tagactgccc cacaatacca ttaccgactc
     2401 ggtagttttt tctttttggt ccggttgata ccggactttc tatcggaaca gcctcgaggt
     2461 taacatcata aatgaaaatc tcaaggcgct tataatgcaa gttaccataa ctaacatatc
     2521 tgacacgaaa tgaaattttt tcttttcttc tcgatatata agttataata aatgatgctc
     2581 tcctcaggtt tataccgaat gcttcggcaa tatcattgcg agaaatccat ttctcctgca
     2641 atttacacca aagagaaacc aagatataca gaggctcacc atcgtaattc tttactgagt
     2701 caggaattac atagtcgcca tttttatttt tagatcccat tttccatgtt ctttttattt
     2761 gataaataaa tcgacataaa aacattcata aacaaaaatt attttaaata aaacttatga
     2821 tattt
//
LOCUS       DAZGOB010000266         2822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGOB010000266 DAZGOB010000000
VERSION     DAZGOB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.268"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..270
                     /locus_tag="ACHLIF_005116"
     CDS             <1..270
                     /locus_tag="ACHLIF_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9608927.1"
                     /translation="VATNNLKLGTFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQVGTP
                     FTLTQSPVSAFAADKLHVTDITRGATYPVLIDSIAVEVNSTDTAA"
     gene            406..585
                     /locus_tag="ACHLIF_005117"
     CDS             406..585
                     /locus_tag="ACHLIF_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9608928.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDVWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            626..898
                     /locus_tag="ACHLIF_005118"
     CDS             626..898
                     /locus_tag="ACHLIF_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9608929.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            975..1190
                     /locus_tag="ACHLIF_005119"
     CDS             975..1190
                     /locus_tag="ACHLIF_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9608930.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGK"
     gene            1195..1539
                     /locus_tag="ACHLIF_005120"
     CDS             1195..1539
                     /locus_tag="ACHLIF_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO9608931.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPIILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1590..2123
                     /locus_tag="ACHLIF_005121"
     CDS             1590..2123
                     /locus_tag="ACHLIF_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9608932.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            2283..2420
                     /locus_tag="ACHLIF_005122"
     CDS             2283..2420
                     /locus_tag="ACHLIF_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608933.1"
                     /translation="MFDFSKLIREIRMMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            2422..2760
                     /locus_tag="ACHLIF_005123"
                     /pseudo
     CDS             2422..2760
                     /locus_tag="ACHLIF_005123"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2732..>2822
                     /locus_tag="ACHLIF_005124"
     CDS             2732..>2822
                     /locus_tag="ACHLIF_005124"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO9608934.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGIISSSEN"
BASE COUNT          736 a          634 c          807 g          645 t
ORIGIN      
        1 gtggcgacaa acaacctgaa actgggaacc tttggcgcat ttgataacga atggcatacg
       61 ctggctttcc gctttgccgg gaataacagc cttcaggtga cgccggttat tgatggtcag
      121 gttggcacac cgttcacgct gacgcagtca ccggtcagtg cctttgcggc ggataaactg
      181 catgtgacag acattaccag aggtgcgact tacccggtac tgatagacag cattgcggtg
      241 gaagtgaaca gcacagacac tgcggcatga taaaaaaacc gccagcgaca ggaatggacg
      301 ctggcggtgg tgatacctat ggagaaaaaa taaaggaacg atactttcgt actctggttt
      361 tttaatgaaa acagttctta ttgtcaacaa taacggaaag aaattatgac atttctgaac
      421 cagttaatgc tgtacttctg tacggtggtc tgtgtgctgt atctcctttc gggtgggtac
      481 agggccatgc gtgacgtctg gcgcagacag attgacaaaa gggccgctga gaaaatcagc
      541 gccagtcagt cagccggaag caaacccgaa gagccgctca tttagcggca actttcttaa
      601 tcacatcttt cgacgagaaa atcccatgtc agaaattaca tccctggtca ctgctgaagc
      661 agtgaaggaa gtcctgcgct ctgaagaagt ccggagcgca ctgaaacaaa aacttcgcca
      721 taacctggaa gcgcgtcttg atgcagaggt tgatgccatt ctggatgagc tgcttggtgt
      781 acaggcagag ccaccgactg aagcgggaga taccaccgca gagagcggtg aagttcagcc
      841 tgaatcaccg gtcgccgatg cgactgaacc tcaacccgaa tcggtcatga tgctgtaacg
      901 gggagtcagg gccatcagta gttaactggt ggctttttta ttgttgtcag cttccggata
      961 acgggagacg gggtatgtac cagatggaaa aaatcacaac gggtgtgtca tacaccacgt
     1021 cagcggtggg gacgggatac tggttactgc agctgctgga caaagtctct ccgtcccagt
     1081 gggtggcaat cggtgtgctg gggagtctgc tgtttggcct gctgacgtat cttactaacc
     1141 tgtatttcaa aatcagagag gaccgtcgta aggcggcgcg gggaaagtaa agcgatgaag
     1201 aaaaaatacg aactggttgt taaagggata aataattacc cggataagat tactgttact
     1261 gtggcactgg aaattggtgg gtatccgtca ctgttgttgc cagatgtggc gattagtctt
     1321 gaccgtactg aaggtgccac gctggagttt tacgaagctg aggcgaaaaa gcaggcgaag
     1381 cagtttttca tggatgttgc tgccgggtta tgtgaagggg atggtccgtt gccggaaaag
     1441 cggcccatca ttttagaggc gcaggatgtg ttgataacct acagaggaaa actaccggga
     1501 ataattactg gttctctgaa gactccaccg ctggcctgaa gacttaatat atccagggat
     1561 ttgaaatcga taaaccctga taaatatcca tgaacgcaaa aatcagatac ggcctgtcgg
     1621 ctgccgttct ggcgctgatt ggtgcagggg cgtctgcgcc tgaaatcctc gaccagtttc
     1681 ttgacgaaaa agaaggtaac cacaccacgg cataccgtga tggtgcgggg atctggacca
     1741 tctgccgcgg tgccatcctg gtggatggta aacctgtcgt cccgggcatg aagttgtcga
     1801 aggaaaaatg cgaccaggtt aacgccattg aacgtgataa ggcgctggca tgggtggaga
     1861 aaaacatcag agtgccactg accgaacccc agaaagcggg gattgcgtca ttctgtccgt
     1921 acaacattgg ccccggtaag tgtttcccgt cgacgtttta cagacggatt aatgccggtg
     1981 accgcagggg agcatgcgag gcgattcgct ggtggattaa ggacggtggc agagactgcc
     2041 gtattcgctc aaacaactgc tacggtcagg tctcacggcg tgaccaggag agcgcgctgg
     2101 cgtgctgggg aattgacaga taagcagaat attttgctga aaaatgcgat ttgctcacgc
     2161 ggacggataa cacgaaatcc tgcgaactga caaaaactaa gtgaataaaa gtaaaaaccc
     2221 cgtttgttgg ctgcaagcgg ggttttgtgt ttcctgactc cggaaaagtc aaaggagaaa
     2281 gtgtgtttga ttttagcaaa ctgattcggg agattcgaat gatggctgaa aaattatcca
     2341 cctggaagtt catccttatc tggctggtgt ttgtgattat ggcttccggt tatttcattg
     2401 gtcagatacg ctggtggtga aatgaaccgc gtactgtgcg tggtcatcat tgccctgctg
     2461 gtggcctgtg gtgcgcttag tctggggctg aatcattacc gtgataacgc cataacctac
     2521 aaagagcagc gcgataaaaa agtcagtgag ctggagctgg caaatgcaac cattactgat
     2581 atgcagcagc gccagcgtga tgttgctgca cttgatgcca gatactcgag ggaattagcc
     2641 gatgcgagag ctgaaaatga aactctgcgc gctgatgttg ccgctggtcg taagcgcctg
     2701 cggatcaacg ccacctgccc cggtaccgtg cgtgaagcca ccggcacctc cggcgtggat
     2761 aatgcaaccg gcccccgact ggcagacacc gctgaacggg attatttcat cctcagagaa
     2821 cg
//
LOCUS       DAZGOB010000267         2761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGOB010000267 DAZGOB010000000
VERSION     DAZGOB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.269"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..281
                     /locus_tag="ACHLIF_005125"
     CDS             <1..281
                     /locus_tag="ACHLIF_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9608935.1"
                     /translation="DGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDS
                     FWVKNIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            288..1029
                     /locus_tag="ACHLIF_005126"
                     /pseudo
     CDS             288..1029
                     /locus_tag="ACHLIF_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1055..1816
                     /locus_tag="ACHLIF_005127"
     CDS             1055..1816
                     /locus_tag="ACHLIF_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9608936.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAPKVTADMMVEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMSGGNLPADPKAAPVTKNDGKA
                     FPQPAGAALPVREAATQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKCRDILRDITATREQQR"
     gene            1849..2130
                     /locus_tag="ACHLIF_005128"
     CDS             1849..2130
                     /locus_tag="ACHLIF_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9608937.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            2127..2354
                     /locus_tag="ACHLIF_005129"
     CDS             2127..2354
                     /locus_tag="ACHLIF_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608938.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            2347..2658
                     /locus_tag="ACHLIF_005130"
     CDS             2347..2658
                     /locus_tag="ACHLIF_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608939.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
BASE COUNT          735 a          572 c          855 g          599 t
ORIGIN      
        1 atgatggcga gatcacgaga ccgaaagaac ccaactggac tgcatgggcc aatgacgtgc
       61 ggacaatgcg gatgctggat ggcagaactc acagacaaat ttgtgaaatg tttgggcgtc
      121 tccagcggga ttcgttctgg gtaaaaaaca tcatgagtcc ggcaaaactc cgggaaaaat
      181 gggatgaact ggttatccgc ctggggcgtt cgcccgcgca gcgttgcgtg aatcacattt
      241 ctgaaccgga cactgaaata ccgccgggat tcagggggtg acgtgtcatg aaaaacattg
      301 cggcagttgg ggttcttgaa cgtattcgca gacttgcacc acaggggggc ggttccaccg
      361 taccggacgg tggaggagtg gcgggaatgg caacttgctg aaggacgaaa acgcagcgag
      421 gagattaacc gcctgaatca tcaggtgcgg gttgaaaaaa tcctgaaccg tgcgggcatc
      481 cagccgcttc acaggaagtg ctcattcggg aactaccggg tgcagaacga cggtcagcgc
      541 catgctctga gccaggcgaa atccattgcc gatgaattga tgaccggatg tacaaacttc
      601 gtgttcagcg gtaaacctgg taccggtaaa aatcacctgg cagcagcgat tggcaatcgg
      661 ctgatggcga aggggagaag cgtgattatc gtcaccgtgt ccgatgtcat gagcgtgttg
      721 catgacggct acgacaacgg ccagtccggg gaaaaatttt tacaggagct ttgtggagtt
      781 gaccttctgg tccttgatga aattggcatg cagcgggata cgcgcaacga gcaggtcacg
      841 ctgaaccaga tagtcgaccg cagaacggct tcgatgcgta gtgtcggaat gctgacgaac
      901 ctgaatcacg cagcgatgag cacactcctc ggagatcggg tgatggaccg tatgaccatg
      961 aatggtggtc gttgggtgaa ttttaactgg gagagctggc ggtcaaacgt tggacgtcag
     1021 ggtatgtgag aatttttgac gaggtaaatt ttcgatggaa actgtattgc atgcactgaa
     1081 agcgatggga aaagccaatt ctgttgaact ggcggcgcgg cttgatatca gccgtgaaga
     1141 agttcttaac gaactgtggg aactcaaaaa aaatggcgtt gttgataaaa cgggtcacac
     1201 ctggtttctg gctgtcgaag gtgaagccgg ggtaaccgaa gggcaggcac tacaacctga
     1261 agcgccggat gtggtaaccg aagaggtcgc tccaaaagtt accgcagaca tgatggttga
     1321 gtttatcggt caggatggtg ctaaaacgtg tgaggaactg gcgggtaagt tcggcgtcag
     1381 tactcgcaag gttgcttcca cgctggcggt ggtaaccgca acggggcggc tggcacgcgt
     1441 taatcagaac ggtaaatttc gttactgcat gtcggggggt aatttaccag cagatccgaa
     1501 agccgcgccg gtaacgaaaa atgatggtaa ggcctttcct cagccagcag gtgctgcgtt
     1561 accagtccgg gaagccgcaa cacaggaaga aataaaaacg gaaagtgtgg cggtcacagt
     1621 gcagtcacag ccgtcgttca ccagaaagca tccggatggt ctgattttac catcgctgca
     1681 tgtggctaac cgcgagctgc gccgggcaaa aggtcaggtt cagaagtggg agcgagtctg
     1741 cgccgcgctg cgggagctga acaagtgccg ggatattctc cgggatatta ccgccaccag
     1801 agaacagcag cggtgagtgg gtggaagacg tggtgccggg cggaaatcat gatactccgg
     1861 cagtgtgcgg gaacgatgaa ggtaaaaagc gttggcgcac ttatcggacg aactgaagcg
     1921 gcagtgagaa cgaaggcacg ggagctgggc atcagcatga tgttacgtgg tgattttcac
     1981 ccgtcggcaa aatattctca gcgtgatatt gagctggcgc ggcaactgca tcagagaggc
     2041 atgcaaagaa gggaaattgc cagaaaatta ggcatgccgc tgcgcatagt gaataactac
     2101 gtttatttcg acaggagggt gtctgcgtga aaatcctgta tcaggattac ggcccggtgg
     2161 ggcaggtggt tatcagcagt actgtaatgg agtttcggaa gcataaccgt gtggtggatg
     2221 ctgtgctgtt aacctgtccg gggatatcgg cgagtcgtgc aggtgtgttt attatgaaga
     2281 cgaaattata tggcagtaag gcgtggataa agaaggcgta tcgtgtagcg ttgcaggagg
     2341 ttaacagtga gtgaaattaa agaaatgccg gtagttcgtg acggatatgg ctactggaca
     2401 catcctgaat atgaaaaatt ctgtgatggt cgggaatata tttcaacgga agagtttaac
     2461 gcctggatgg aggaaaataa tcttcaatac gtcctctgct tcagagatga aggatgtgct
     2521 gaccttgatg cgtgtgatgc tgatatttct gcatgggaac cggaacgacc agagggcgat
     2581 ggctggttta ttggttccat tcatgatacg gaagatggcc cggtttgtgt ctggttgcga
     2641 aataaggctg aagcataaag gcgataaacc aactaacaac taaatactga agatttaaat
     2701 cagaaacgat ttttattaaa tccttaaccg gagggattcc tgcaccctca gaacatcagg
     2761 a
//
LOCUS       DAZGOB010000268         2722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGOB010000268 DAZGOB010000000
VERSION     DAZGOB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.270"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(60..1250)
                     /gene="aspC"
                     /locus_tag="ACHLIF_005131"
     CDS             complement(60..1250)
                     /gene="aspC"
                     /locus_tag="ACHLIF_005131"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO9608940.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMALLCEAI
                     VAVL"
     gene            complement(1435..2523)
                     /gene="ompF"
                     /locus_tag="ACHLIF_005132"
     CDS             complement(1435..2523)
                     /gene="ompF"
                     /locus_tag="ACHLIF_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO9608941.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          689 a          664 c          648 g          721 t
ORIGIN      
        1 atgttgcgtt tgtcatcagt ctcagcccgc ttttcagcgg gcttcattgt ttttaatgct
       61 tacagcactg ccacaatcgc ttcgcacagc agagccatgt tatctggtgt catcccggcc
      121 acatttacgc gaccagaagc aaccgcatat acgccaaact cttcgcgcag acgcagcact
      181 tgttcttttg tcaggccact gaaggagaac atgccgttct gtttgatgat aaagctgaag
      241 tcgcggtttg cgcctttttc ctgcagcgta ttgacgaaca actgacgcat acgctgaata
      301 cgctggcgca tatcagtcag ctcttgttcc caaatcgcac gtaacgcatc gttgctcagg
      361 atggtggcaa caacagaagc gccgtgtgct ggtgggttag agtagttagc gcgaatcgcc
      421 gctttcattt ggctgaatgc gcgatcaacg gtttcactgt cggcagcaac cagagtacaa
      481 gcgccaacac gctcgttgta caggccaaag tttttagagt aggaactggc aacaatcagc
      541 tctttatgca tagccgcgaa agcgcgcagt ccttcagcat cttcttccag accacgggca
      601 aaaccctggt aagcgaagtc aaacagcggt aaccagcctt tctcaactga gagttgtgcc
      661 agtgtctgcc attgttccag cgtagggtcg ataccggttg ggttatggca gcagccatgg
      721 aacagcacta cgtcgccagc ctgagcttcg ttcaggctgt taatcagtgc atcgaagtca
      781 agggtgtggt tttctgcatc ataataagcg tattcacgaa cttccagacc tgcagagtta
      841 aagacgctct tatggttcgg ccagcttggg ttgctcaccc acactcgctt aacgctggta
      901 ttttttgcca ggaaatcggc agctacgcgt agtgcgccag tgccccccgg agtctgtgcc
      961 gtgcgagcac gtttgtcatt gatcagggcg ctacctttac caaacagcag ttcctgagtg
     1021 cagcgaccaa attcagggat gccgtcaatg ccgaggtaat ttttggtggt ttcattttcg
     1081 agcagatact gttcagcctt tttcacgctg gtcaataccg gggttttgcc cgtctcatct
     1141 ttatagacac caatcccgag gttaattttg ccgggacgtt catcggcacg aaacagatcg
     1201 gccaggccca gaatcgggtc ggcaggagcg gcggtaatgt tctcaaacat gacgaggttc
     1261 cattatggtt acagaaggga agtccgctat cagggtaacg ggagatttac aaaattccaa
     1321 ctattactga tgaaaacgca ggctgttttt gcaagacgtg agattgctct ggaaggcata
     1381 aaaaaaacag gaccaaagtc ctgttttttc ggcatttaac aaagaggtgt gctattagaa
     1441 ctggtaaacg atacccacag caacggtgtc gtcagaacct acgcccagtt tgttgtcaga
     1501 atcgatctgg ttgatgatgt agtcaacata ggtggacatg tttttgttga agtagtaggt
     1561 tgcgcccact tcaaagtagt tcaccagatc aacatcaccg ataccttcta cgtctttcgc
     1621 tttagatttg gtgtaagcga tggacggacg cagaccgaaa tcgaactggt attgcgcaac
     1681 taacagaacg tcttgcgttt tgttggcgaa gccgctggtg tttgtaaatt tattagtgat
     1741 cggcgtagcg ttacgggttt caccgtagtt cgctgccagg tagatgttgt tcgcgtcgta
     1801 cttcagacca gtagcccact gttcagcttt tttaccgttg ccaagaggtt gagcttcttg
     1861 caggttggta cggtcagctg caccataagc accaacgata ccaaagcctt cgtattcgta
     1921 gctgatagaa ccgccaacac cgtcgccgtt agaacggcgt gcagtgtcac gctcgttttt
     1981 acccaggtac tgaacagcga agttcaggcc atcaaccaga ccaaagaagt tggagttacg
     2041 ataggtagca acgccgccaa cacgaccaac gaagaagtca tcgctgtatg cagtatcacc
     2101 accaaattct ggcagcatat cggtgtaacc cagtgcatca taaaccacac cgtagttacg
     2161 gccgtaatcg aaagaaccaa cgtcagcgta tttaagaccc gcgaatgcca gacgcgtttt
     2221 gttaccagtt tgagcgtcag cgccttcaga gttgttaccc tggaagttat attcccactg
     2281 accataaccg gtcagatcgg aattgatttg agtttcccct ttaaaaccaa gacgggcata
     2341 ggtcatgtcg ccattgccac cgtaactgtt ttcaccgtta cccttggaaa aataatgcag
     2401 accaacagct ttaccgtaca gatctacttt gttgccatct ttgttataga tttctgcagc
     2461 gtttgcagta cctgctacta acagagcagg gacgatcact gccagaatat tgcgcttcat
     2521 cattatttat taccctcatg gtttttttta tgacacctgc cactgccgtc aataagttct
     2581 gtcaataaaa atttacggaa ctattgatga gagtttggtg tctttatgtg tctgcaggca
     2641 tctttccatt caaactaacg tttcgctacc gtgaaagtgc tacaaagata aagatttagt
     2701 ttcaaaaaga aaaaatatgt aa
//
LOCUS       DAZGOB010000269         2715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGOB010000269 DAZGOB010000000
VERSION     DAZGOB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.271"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..278)
                     /locus_tag="ACHLIF_005133"
     CDS             complement(<1..278)
                     /locus_tag="ACHLIF_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO9608942.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALRE"
     gene            complement(448..524)
                     /locus_tag="ACHLIF_005134"
     tRNA            complement(448..524)
                     /locus_tag="ACHLIF_005134"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(488..490),aa:Arg,seq:tct)
     gene            complement(538..614)
                     /locus_tag="ACHLIF_005135"
     tRNA            complement(538..614)
                     /locus_tag="ACHLIF_005135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(578..580),aa:Arg,seq:tcg)
     gene            complement(624..699)
                     /locus_tag="ACHLIF_005136"
     tRNA            complement(624..699)
                     /locus_tag="ACHLIF_005136"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(664..666),aa:Ile,seq:cat)
     gene            complement(728..1441)
                     /locus_tag="ACHLIF_005137"
     CDS             complement(728..1441)
                     /locus_tag="ACHLIF_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9608943.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     ATDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRHGYGQAIAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(1576..1773)
                     /locus_tag="ACHLIF_005138"
     CDS             complement(1576..1773)
                     /locus_tag="ACHLIF_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9608944.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(2015..2545)
                     /locus_tag="ACHLIF_005139"
     CDS             complement(2015..2545)
                     /locus_tag="ACHLIF_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO9608945.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(2554..>2715)
                     /locus_tag="ACHLIF_005140"
     CDS             complement(2554..>2715)
                     /locus_tag="ACHLIF_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9608946.1"
                     /translation="RRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQPVSGGRLG
                     VKIYKIESE"
BASE COUNT          727 a          725 c          557 g          706 t
ORIGIN      
        1 atctcgcgta acgcggcacg acgaccaatt ttaaaattgg tatccagctg accgataata
       61 gttgcgctaa tcgcattaat ttccgaggta aacgcagaca acgcaggctg attacgtaag
      121 acctgttcga tcttcgcttt ctcggatgcc tcgcattcac catctgcata ggccaccagg
      181 tatgcagcgt taatcaccgc ctgtgccaga tcgcgtttct caaacttttt aatttccgct
      241 gccgctctgc gggttttctt tttgaagatt ccaaacatcg tgacgttcct ttgggtgggt
      301 gagccaacgc ccgggagcga tctgcccaca gagaaagtca cactgaccac tccgtaagct
      361 cacccccgaa aggctctgtg gttgatatgc gccgggcgtg gcgcagatac aaaaaaggcc
      421 cgccgaagcg agcctggaaa ataagtgtgg cgcgttgtag tggagtctaa ccactgaccg
      481 attgcttaga aggcaattgc tctgtccgac tgagctaaca acgcatgatg ctgataatgg
      541 accgccatcg gggacttgac ccccgcgcag ccagcttcga aggctggcgc tctgtcccga
      601 tgagctaata gcggtatgta atatggtggc ccttgctgga tttgaaccag cgacctggcg
      661 attatgagtc gctcgctctc accactgagc taaagggccg agccaaaaaa taataatcag
      721 atgaaaatca ataatcaagc ccttgcctgg atacatatct gtctggcggg aagccataat
      781 agcggtgaaa tacagaaata aagtaggacc tgcttgaata accgcatttt tctgctatag
      841 cctgtccata tccatgccgg gaacataaca tatttacagc aacacgcatc cgctcttcca
      901 gcaacaagcg actgaacatg cccccttcat ttttcagttt tgtctttaac aaactctcac
      961 tcatatgcaa ctgtagagca atcgcaccaa gcgtccagct tgctgatata tctgtctgaa
     1021 ttatcgccct gactttggca cttatgctgg ataaacatcc acttaaaaac aatgacatcc
     1081 gttcatctgt ttcaaacaga gacaggcagg ccatcataag aaacatatcc gtggcctctc
     1141 cggaaaatcc ctggctggta attaaagccg cagccaacgc aggattgttg ggttccagca
     1201 acaggtaaag cggaatgtca gtcagacgag tcctcgtcag cttatgctga ctttccagat
     1261 attgacttac gacggattcg cttatatcga caattttaac tttgccataa tgcataagga
     1321 aaagctccct gatgcatttg gtggccagaa cgactgagcc tggcttaagt gacaacgtat
     1381 ccttttcaag aaaaatatta attggggagc aaaccatgat aactgaacag acaacagcca
     1441 ttataatttt actttcatta gcaattggtt agctcaatta tagccccaaa aggtaaatta
     1501 tcatcaacac ataagcaaag gactgacagg tgtcgccccc ccaccagccg cccattcacc
     1561 acaaataaaa agccttcagg actgaaggcg tctgtaacaa ccgcactgat agtctgccag
     1621 acccgccata acaagctggg tcagtattaa ctggcagcgt tcgcgtgaaa ggtaagtatt
     1681 ctgcgcaacc tccccgactg tcgccggttc ggtaacgctt aattcattaa acaccactct
     1741 ggcggtttcg gtcatatcct gctgtttcag catgtctttt tccctttttc ggttaacgtg
     1801 acacaccaat aactcttgtc gaaaaagcca gcaagctgaa agaacggtat taataaccac
     1861 cagcgaattt attgcgctgc tgtatattac ggacacaaaa aaaccacctt ccggtggctt
     1921 ccttgtgcga aaaaacttgc atttcgcctc gcgatacagc tttgcgaagc ttacaggaat
     1981 tcaagctgtt tctgcgtaaa aaagcaagct ttttttatcg aaatgaatcg tgcataggta
     2041 cataaagcat gtgttcagcc acggctaacc aacctgcaat acgtttctca catgtgctga
     2101 aacaccattc cgggtgagta cgatttaaac attctgccat ttttctctta ctcattcccc
     2161 gtccttcgta cctttgccgg agaatattga ttagcccggg atattcccca agcacctcac
     2221 tgataacgcg atcaataatc aacgcctctg tgtctgtaca atgtgacaac cagctcttct
     2281 gcttccctct ggtcatatcc cgaaaaaatg cctcaagttc cggtttttcc agcccggatt
     2341 tcttcatgct gcgtaaaacc tcattaactg ctgttttcgt cagctttttc gaaaccagta
     2401 accggttaaa catatttccg gatttacccc caccgatata cgaccaccgc ccccacatcc
     2461 gtaatttccc ctggatccag actgcttcca gcgtgttcag gcgtaaatgt tcgccgcttt
     2521 tgcctgtaat ttccggatat atcatattta cgctcactca ctctcaattt tgtaaatctt
     2581 cacacccagc cgtccaccag atactggctg accgcgcaca atattgattt catcaaactg
     2641 ctcgtcatca atgagcactt ccgcatgcgt cagcgcatcc agcggtgctt tcagaatgtt
     2701 gtccaggtca cggcg
//
LOCUS       DAZGOB010000270         2674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGOB010000270 DAZGOB010000000
VERSION     DAZGOB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.272"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            188..277
                     /locus_tag="ACHLIF_005141"
                     /pseudo
     CDS             188..277
                     /locus_tag="ACHLIF_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
     gene            282..690
                     /locus_tag="ACHLIF_005142"
                     /pseudo
     CDS             282..690
                     /locus_tag="ACHLIF_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1337..2314
                     /locus_tag="ACHLIF_005143"
     CDS             1337..2314
                     /locus_tag="ACHLIF_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO9608947.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            2498..>2674
                     /locus_tag="ACHLIF_005144"
     CDS             2498..>2674
                     /locus_tag="ACHLIF_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9608948.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREG"
BASE COUNT          728 a          611 c          649 g          686 t
ORIGIN      
        1 ggctgagagc cgcatcggag aagttgcgtc cattttcagg ggaatggggt aaaaaaccat
       61 cagtgatatg cagtatcaat cgataagatc catttaattg acggcggtgc actcatggca
      121 cgcaggcagt gttgaataaa catccgtttt tgggtgtttt tttaatcttt ttgggattta
      181 aattcctatc gatgaacaat taattatcgc tgttgtagct gtgggtaaac gtgagcgtag
      241 tgacgtttat aatcttgcca gtgaaagaat gagataatta acggcggatt atcggttcag
      301 ccctctcatt aaccgctgat gctgagttgg tgtgtatggc tttgcgtaat gcactggaaa
      361 cacgaccgcg tgatggtcgc ctgctgtttc attcagatca aggtgttcag tataaaagca
      421 ataaatacag aaagttactc tggcgttatg gggtaatgca aagtatgagt cgcagaggga
      481 attgtctgga taattcgccg atggaaaaag tctttcgcag ccttaaaagt gaatggcttc
      541 cgaaaggtgg ttatggtgat tgccatgcag tacgcgaata aatcagtgga taaatgctta
      601 ttacaacgtt tatcgacctc atacaaacaa tggtggttta tcgccttgcc gcatgaagaa
      661 aaatggaaac aggttattcc ggtgtcctga ttttatgatc cactacacat tcctgacgaa
      721 cgccactgca taaatcacat tgcacatcct cagagagtac gcagtatagg aggggtatat
      781 atacattatc ggtatatcag gcgacttctg tctgtatgtt gtcggtgctc gccgcagacc
      841 ggtgaccggg cgaagcccgc gaaccggcga ggaagcgcaa tgtatctgta gttacagtga
      901 gcagatacgc cctgaagctt cctcataaaa cagttgtgta aactgttttc ccggaatgat
      961 ttatccacat atccggcggc ccgatccagt aaacagatcc ataaatgatc aagaaatgat
     1021 ccattaaaga tccccatatc gctgcaaacc ttgtcactca tgctccggga ccacgatcac
     1081 ataagctgtg gcatgtttct gataaactgt agcaggatca tgataagctg tattcagtaa
     1141 tgtatatact gtagtaagtt aatgacataa attatggtca gtacgccagg ctcagctgtt
     1201 aaatacagac tgcaggtttt tcttcagtca gttgacgggg ttctgacact caattttctg
     1261 tttattcttt tactgtccac aggcaggagg ctttctggaa aacgaaaatt cagacatcaa
     1321 aaaactgttc ggcgaggtgg ataagtcgtc cggtgagctg gtgacactga caccaaacaa
     1381 taacaacacc gtacaacctg tggcgctgat gcgtctgggc gtttttgtac cgacccttaa
     1441 atcactgaag aacagtaaaa aaaatacact gtcacgtact gatgccacgg aagagctgac
     1501 acgtctttcc ctggcccgtg ctgagggatt tgataaggtt gagatcaccg gcccccgcct
     1561 ggatatggat aacgatttca agacctgggt gggaatcatt cattcctttg cccgccataa
     1621 cgtaattggt gacaaagttg aactgccttt tgtcgagttt gcaaaactgt gtggtatacc
     1681 ttcaagccag tcatcccgca ggctgcgtga gcgcatcagc ccttccctga aacgcattgc
     1741 cggtaccgtg atctcctttt cccgcaccga tgagaagcac acccgggaat atatcaccca
     1801 tctggtacag tcagcctact acgatactga acgggatatt gttcagttac aggctgatcc
     1861 ccgccttttt gaactgtacc agtttgaccg gaaagtgctg ctgcagctca aggccatcaa
     1921 tgccctgaag cgacgggagt ccgcccaggc actttacacc tttatagaga gcctgccccg
     1981 ggatccggca ccgatatcgc tggcgcggct gcgtgcccgc ctcaatctga agtctcctgt
     2041 attttcccag aaccagacgg tcagacgggc aatggagcag ttgcgcgaga ttggatatct
     2101 tgattacacg gagatccagc gggggcggac aaaattcttc tgtattcact accggcgtcc
     2161 ccggttaaaa gcgccgaatg atgagagtaa ggaaaatccg ttgccacctt cacctgcgga
     2221 aaaagtcagt ccggagatgg cggagaaact tgccctgctt gaaaaactgg gcatcacgct
     2281 ggatgacctg gaaaaactct tcaaatcccg ctgaacataa actgtagtca gtgaagagtg
     2341 ctcctttact gactacagct tatattatca ggtgcagtga gtggtctgct cactgcagtt
     2401 tatatgtaag cgtacagcct gaaccgtctg gtcagaatct gacgaattag acaaagtggt
     2461 gtccaccaaa taagtagtgg gaaccaaagt gtcagatatg cagaaaaatg tgactcccgg
     2521 caggcgaaaa ggctgcccta attatcctcc cgaatttaaa cagcagctcg ttgctgcctc
     2581 ctgtgaaccc gggatatcca tctcaaaact tgctcttgaa aatggcatta acgccaatct
     2641 gttgttcaaa tggcgacaac aatggcgcga ggga
//
LOCUS       DAZGOB010000271         2656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGOB010000271 DAZGOB010000000
VERSION     DAZGOB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.273"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2379)
                     /locus_tag="ACHLIF_005145"
     CDS             complement(<1..2379)
                     /locus_tag="ACHLIF_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9608949.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYVAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVT
                     VDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQQAVNDAVANIPQPADGKSLTPDDV
                     RPMLEQMVKEAVSHIPVPRDGRDYDPEVLKQAVNDAVANIPVPADGKSITPDDVRPML
                     EQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPVPADGKSITPDDVRPMLEQMV
                     KEAVSHIPVPRDGRDYDPDVLQKAVLEAVNALPAPQDGRDATALEVLPAIDDQKSFPR
                     GTYATHLGGFWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVIRHSSGQ"
     gene            complement(2325..2546)
                     /locus_tag="ACHLIF_005146"
     CDS             complement(2325..2546)
                     /locus_tag="ACHLIF_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608950.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
BASE COUNT          544 a          880 c          628 g          604 t
ORIGIN      
        1 ctggccactg ctgtgccgga tcaccacaga gaacaaccgc tcccccgtca tactgacgtc
       61 aatatccgcc accccgtcaa ccaggcattc ccatccccgc attccgtgcg ttttttcata
      121 cgcccgccag aatcctccca ggtgtgtggc atacgtgccc cggggaaagg atttttgatc
      181 gtcaatagcg gggagcactt ccagagccgt ggcatcacgc ccgtcctgcg gagccggcag
      241 ggcattcacc gcctccagaa cagccttctg cagaacatcg ggatcatagt cacgaccatc
      301 acgcggaaca ggaatatggc ttaccgcctc tttcaccatc tgttcaagca tcggacgcac
      361 atcatcgggg gtaatacttt tgccgtccgc cggtaccgga atattcgcga ccgcatcatt
      421 caccgccttc tgcagtactt ccggatcgta atcacgacca tcacgcggaa caggaatatg
      481 gcttaccgcc tctttcacca tctgctcaag catcggacgc acatcatcgg gggtaatact
      541 tttgccgtcc gccggtaccg gaatattcgc aaccgcatca ttcaccgcct gcttcagtac
      601 ttccggatcg taatcacgac catcacgcgg aacaggaata tggcttaccg cctccttcac
      661 catctgttca agcatcggac gcacatcatc cggggtgaga cttttaccgt ccgccggctg
      721 cggaatattt gcgaccgcat cattcaccgc ctgctgcagt acatccggat cataatcacg
      781 accatcacgc ggtaccggaa tggcccccac agcgtcatcc accatcgcct gcagaaccgg
      841 acgcacctca tccaccgtca catgcttctg taataccgcc gacagggaag tcagtttctc
      901 ttcaaacgct tgtgcctgcg aggccatctt cccctcaaat gtgcgctgta aatccgccag
      961 cactgtggag aattcttctc ccagtgcacg aataatggac agttcccgtt ccgtcatttt
     1021 ctcagtatcc cccctgaaca tcgctttcac cgcatcatgc tctgtttcac tgattgcctt
     1081 attaccgtca gatgcgccgt caggcagctg tgatgaaact gttttcccgg cagacgcgaa
     1141 cggatcctca cgggcatcac gacgggacag cgcctccaga ctgtagttct gctgctgaag
     1201 atacagtgca tcaccgccgg ccaggggcgg caggttctcc cgtttacggg cctcattggg
     1261 cgtgagaagc gtatttttca ccgcatcccc cagcgttttc atgcgccgct cactgtccat
     1321 tctcagcagc gtggtgacat caaattctgt actctcgttt tcccccgttt ccagcgcctc
     1381 atccagtaac agttcaatgg actcaatcag cgtctgcagg cactgggaat aatactgctg
     1441 ctccagcgcc tccacgttgt cactggaagg cggttgtccc acgccaatct tgtaggccgg
     1501 gacacggaac accgaacaga caatttcagc ggtcatcttc agttgttcca ccgtctgcgc
     1561 atccacaggt gaaaacgtcg tggggttgta ttttgccccg ttgctcagaa tggccgtttt
     1621 ccccgcattt tcgcctgtat acccgctgtc ccagttgctc ttcagttttt tcgcattttc
     1681 ttccgtaata ctgccgggga tctcaatcac cccggacggc ctgccgccat ttctgaaaaa
     1741 agacgtcgaa ttttcctgaa tatgatgccc ctgcgtggcc gccagcccgg cgacatacac
     1801 cggcggcaat cctataagcg gatgaaaaaa acagttaaac cggtcgtgga tcacttcccg
     1861 ggcaggcacc gtcaccgcct ccgtgatccc gcagttccgg tccggcgtga tgcggtagaa
     1921 cacctcgccg tcatccgcca ccagaggttc aacccggctc cagtccagaa tacgcagttc
     1981 tttgatctgc ccccgggcat tacggatttt cagcaccacc gtattgccat gacgcagttt
     2041 ggcgttcagc cacagttcaa aaaactggat gcggttctgc tgggcgttgg gacgacgaca
     2101 gaggcgggca atatcccccc ggcgcgtttc cctgcgtatc ccatgcgcat ccgtctgcat
     2161 aagacgcagc cgcattttgg cgatatcctg ggatatcagc gaaatacatg caaacaccgc
     2221 atgaaaggag aggacggctt caggatcggc tttcacgccc tgctgccagg cgccggaaaa
     2281 gggctcagcc accgcctgaa acaggctggt ccagcccgcc tctcttacgt cacgtcctga
     2341 tttctggttt tttcgggttc gccgtaaaag gttccacatt cgccatgctc cgcatcacgt
     2401 ttctttttct gacctgccgg acgtcgcacc gtgatgtact ccgcctttcc caggcgaacc
     2461 agcacctccg cacacggctg tgccacatca cggatatccc cggcccgggc atcatgcgtg
     2521 ccctgcagat atcggatctt tgccataacc tgttacggga ggcgcacgcc tcccgtcctc
     2581 cttatcagac tcagccgccg gacgcactgc cgtagttcac tccggtgatc accgccaccg
     2641 ccacaccgcc gtcatc
//
LOCUS       DAZGOB010000272         2653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGOB010000272 DAZGOB010000000
VERSION     DAZGOB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.274"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1353)
                     /locus_tag="ACHLIF_005147"
     CDS             complement(<1..1353)
                     /locus_tag="ACHLIF_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFO9608951.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     S"
     gene            complement(1442..2251)
                     /locus_tag="ACHLIF_005148"
     CDS             complement(1442..2251)
                     /locus_tag="ACHLIF_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9608952.1"
                     /translation="MIHLVNKLINFGFTRTDAQVYISLLKNGRSSGYKIAKEISLSRS
                     SVYSSIDNLYKNGYIFLVDGETKEYEAKSPELILSQIEKSTIDNIKILKKELSQMMFQ
                     NEREFIYNVSGFDNLHQKAKEMLSSAECEIYLNTDFDLHFLKNEICEALNRHVRVIVF
                     SFNKLITPDPRVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSNNRLL
                     INIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
BASE COUNT          839 a          491 c          470 g          853 t
ORIGIN      
        1 gcttaaatca tgagggtatt cagttaattg tgtaatttta attgcaacat tattaaatgt
       61 gccataaaac tcacacgggc cataaaataa tgcagtaaaa ccattgttat tttcttgaaa
      121 catattttcg acaaaagccg gtatggttct tgctgtattc ggtacgcagc agacagcagc
      181 ctcctcatga gtaggggagt atttatatct ggtattaaac tgattactgt ccggatgttt
      241 cctttcattt gcttcataac aattatcagt cttacagtac attatcgcgt gaagtatggg
      301 atgctgcatg ccaaaactag cattatagaa tagccactca gcatcatcgg ccagaccaat
      361 attagtggtc agttttatta acataacata agtgctaaat agttcatgaa cagaacaata
      421 ttcatagcct gtataagtgg cattagctgt gcgtcctgta atccactcat caccaatggg
      481 agcgccagat ggtgtaagat aatatttcaa ctgggataaa aaaatatcta atgcagtttg
      541 atactgcggc cgttggctcg ctgcgatggc taatgcccta atatgctcat acgtatgtac
      601 gccatgacct ttaagaaggt aaccagggtc tatgacattt ttatattgca gatcttgctc
      661 cgacacgcat cctgcggaat aatcttcata cagccacaag caatatgaga gatattcaat
      721 gttattggtt atttcataaa ggcggtagaa aacatctgtt attgttaatc cgtggctatg
      781 tccagcaaaa ccctgttcca caacgaaagg atgtgacgtt tctttagggt agccagccat
      841 aatgcattgt gcggcttggg taagagaagt taataaactc tcattacggg tggactcata
      901 acatccaagc acaaaacgca aggcagtagc ttgtgcccag aactctccat tttctgcttt
      961 acagttaaat tttaatgatg aatcataaat tccaatatag ccatcatcat cttgcgtatc
     1021 gataagttcc aaacataaat cgctcgcttt ttcctgccat tctttattat ctaacaagta
     1081 ggcactgcgg caatatccat ccttccaatt cgattgagtt tcactatccc accaataata
     1141 ttgcatcatg ttatcatcgg gattattctg ttttttaata acgccatctt cagcagatac
     1201 agtgcgtgga gttatacgat ctcttccata tattttatgc tccgtaataa gatggggaac
     1261 taatttatcc agttctccta tatgaccgtt catataccat tccatcttat tctttaacca
     1321 accagtgggc tttatatcgt taaaaaaaat cataaatata cctttgcata aaaaagttta
     1381 gacgttataa aagacaaata gattgtgatc gcctacagac gatcacaatg aaaggaaaat
     1441 attaacggtt tttgctatca ctcaaatgtt tttcatcctg caccatatca ttgccagatt
     1501 catgcaaagt atttattaat gcattggcat ctggatattg ttgctgatac ttggttagat
     1561 atatatcact atggatatgt tcggcaatta tattgataag caagcgatta ttcgagtata
     1621 accccagtga ttcagctcgt tgtgaaaaaa ccattactgt cttcatatca acaacaagca
     1681 taaaacggtg agaagggtaa tattcttctt catggttaaa gcggtaatac agttctacac
     1741 gtgggtcggg agtaataagt ttattaaatg aaaaaacaat aactcgaaca tgtcgattca
     1801 gagcctcaca aatttcattt ttcaaaaaat gtaagtcaaa atccgtgttg aggtaaattt
     1861 cacactctgc agaagaaagc atctctttag ctttctgatg taagttatcg aaaccagaga
     1921 cattataaat gaattcacgt tcattctgaa acatcatctg tgataattct ttttttagaa
     1981 tttttatatt gtcaatagtg cttttttcaa tttgtgaaag tatgagttca ggagattttg
     2041 cttcatattc tttagtttca ccatcgacaa gaaagatata accgttcttg taaagattat
     2101 caatagatga gtatacagac gaacgagaaa gtgaaatttc ttttgctatc ttgtaaccac
     2161 tgctacgacc gtttttcaac agactgatgt aaacctgtgc atcggtgcga gtgaaaccaa
     2221 aatttatcaa tttatttacc aaatgtatca tagacgcgct cctgaaatga aaatgaaatc
     2281 ttaccaattt taacattatt aattaacatt ttcgaaaaaa ttgctgagct aaaatgtgga
     2341 tttatcgtaa ttcatcctca agctggttac aggtctacag tacgtaagca ttgtgaactg
     2401 cgaatctgaa tgccctccaa ctatggtcag aagacatgct ctgacctaaa tagtaggctc
     2461 actgagtaag cggtgttatc aacggtttat tcatatatgc tcttttttat ggatggaatt
     2521 ttgtaactca attaccttga taagaatcag ttcgtggagt taatgacatt taccaataga
     2581 cagttcgagc agtaagaagc cttatggaat tgtaaggttt aataccgtta ttcaacctgg
     2641 ggttattgtc ata
//
LOCUS       DAZGOB010000273         2636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGOB010000273 DAZGOB010000000
VERSION     DAZGOB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.275"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            478..621
                     /locus_tag="ACHLIF_005149"
     CDS             478..621
                     /locus_tag="ACHLIF_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO9608953.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            634..843
                     /gene="cspE"
                     /locus_tag="ACHLIF_005150"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             634..843
                     /gene="cspE"
                     /locus_tag="ACHLIF_005150"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO9608954.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            1009..1818
                     /gene="rlmA"
                     /locus_tag="ACHLIF_005151"
     CDS             1009..1818
                     /gene="rlmA"
                     /locus_tag="ACHLIF_005151"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO9608955.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1815..2381)
                     /gene="mntP"
                     /locus_tag="ACHLIF_005152"
     CDS             complement(1815..2381)
                     /gene="mntP"
                     /locus_tag="ACHLIF_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO9608956.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          694 a          616 c          629 g          697 t
ORIGIN      
        1 cggatttgtg attaagcgtg gtattattta ttagcgaaac gtttctctct tgattttttg
       61 ctcattcatc aatttttctt attttaaatt ttcaatccct ttggggattg acttctcttt
      121 agggtaattg atagccgtta actgactgtt ttatgagaaa aagtgatata actttttatt
      181 cattgcatag caaaaaatgt gatattgcac gcactatgta ataacttctc ccactggcct
      241 ggaacaactg aacttattga actatgttag aaaatacgcc agtttaagta tctgcctgaa
      301 ctggcaaggt taagcacaat gatatatcgg cgcgtattcc gttgcataag tgtgcaaaaa
      361 aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac atgtttaaaa atggcttgcc
      421 ataattaacg ttgtatgtga taacagattt cgggttaaac gaggtacagt tctgtttatg
      481 tgtggcattt tcagtaaaga agtcctgagt aaacacgttg acgttgaata ccgcttctct
      541 gccgagcctt atattggtgc ctcatgcagt aatgtgtcag ttttatctat gttatgcctg
      601 cgggcgaaga aaacaatcta aggaattttt caaatggcaa agattaaagg tcaggttaag
      661 tggttcaacg agtctaaagg ttttggcttc attactccgg ctgatggcag caaagatgtt
      721 ttcgtacact tctccgctat ccagggtaat ggcttcaaaa ctctggctga aggtcagaac
      781 gttgagttcg aaattcagga cggccagaaa ggtccggcag ctgttaacgt aacagctatc
      841 tgatcgaatc cactgatctg aagtgtgaat acgcttcaat ctcgctataa agcctcgtcg
      901 aatgcgaggc tttttactat gctttatctt cgctcctggc gttcggatat ttgcccgccg
      961 cgtgattcgc gttacacttg cggcctttag tatcctgccg gagttgtcat gtcgttttcc
     1021 tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca gctatatctg tccccagcga
     1081 catcagtttg atatggcgaa agaagggtat gtcaatctgc tgcccgttca gcataaacgg
     1141 tctcgtgatc cgggcgacag cgcggaaatg atacaagcac gccgcgcatt cttagatgcc
     1201 ggacattatc agccgctgcg tgatgcaatt gtcgcccaac tgcgggaacg gcttgatgaa
     1261 aaggccacgg cggtgctgga tattggctgt ggtgaagggt attacacaca cgcatttgcc
     1321 gatgtgttgc ccgaaatcac cacgtttggc ctggatgtct cgaaggtagc gataaaagcg
     1381 gcggcgaaac gctatccgca ggtcactctt tgtgtcgctt ccagccaccg tttgccgttt
     1441 tccgatacca gtatggacgc cataatacgt atttacgcgc cgtgtaaagc agaagaatta
     1501 gcacgagtag tgaagcccgg cggctgggtc attactgcca cgccgggacc gcgacatttg
     1561 atggagctga aggggctgat ttacaatgaa gtacatcttc atgcacctca tgcagaacaa
     1621 ctggaaggtt ttacattaca gcagagtgat gagttgtgtt atccgatgcg tcttcgcggt
     1681 gatgaagccg tcgcattatt gcagatgacg ccgtttgcct ggcgtgcgaa gccagaagtc
     1741 tggcaaacac tggcagcaaa agaagtgttc gactgccaga cggactttaa tattcacctc
     1801 tggcagcgtt cttattaacc gtggaagtgc gtccagagga tctggacgcc gatgccgatc
     1861 agcaccagcc cgccgagaat ttccgctttt ttcccaataa ttgagccgat aaagcgacca
     1921 accatcatcc ctaatgtcga cataatcaag gttgcacaac caatggccaa tgcggtcgcg
     1981 ataatgttga cctgcaggaa agcaagacca acacccacag ccatggcatc caggctggtg
     2041 gcaatcgcgg tggttaccag tagccagaaa ccgtgccgac ggcgcggctc ttcatcttca
     2101 tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc cgaggaatat cagcagcaca
     2161 aacgcaatcc agtggttcca ttcaaggaca aaccggctgg ctaacatgcc cattccccag
     2221 ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa ggccggttcg caatgcttca
     2281 gaaaatttcg gtttatggag ggtggcacct ttaccgattg atgcagcaaa tgcatccatc
     2341 gacataccaa acgcaagaag aacagtagca gtgatattca tgacaatgtc ctgaccgggg
     2401 tatccataaa acacatcatc gcccccagca aacatgcata catgaacaac aagccgcgat
     2461 ggttttcgcg ttcatgcagg catagcgatt gatgtgcata tggtctcgcc tgactgaaag
     2521 tattcagtcc gtacgtgcca cgttttgcaa cgagtatgtt gacacgtaca tttccggaat
     2581 ctggaaatcg gctactcccc aaatgagggc gcaaccttaa catattttga gaatat
//
LOCUS       DAZGOB010000274         2612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGOB010000274 DAZGOB010000000
VERSION     DAZGOB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.276"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(201..1559)
                     /locus_tag="ACHLIF_005153"
     CDS             complement(201..1559)
                     /locus_tag="ACHLIF_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO9608957.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            1841..1972
                     /locus_tag="ACHLIF_005154"
                     /pseudo
     CDS             1841..1972
                     /locus_tag="ACHLIF_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2292..2585)
                     /locus_tag="ACHLIF_005155"
     CDS             complement(2292..2585)
                     /locus_tag="ACHLIF_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFO9608958.1"
                     /translation="MVKLLTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVI
                     EEAVNALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          649 a          654 c          600 g          709 t
ORIGIN      
        1 tttgttcgga ctgcttatgg cctttttcca ctggtgtagc aggaaagtta accggctgcc
       61 accatgagat gacgtgtaaa ccgtgcaggc atgttttcac gtggtgacgc tttcattggg
      121 ggtctacgcc ggaaccccaa tattgttcac atacatgaat ttgaaaaact aagcgatact
      181 gctgtccgta ccttataaac ttatttcaac gcttccggca gcgtcacaat ccacagttca
      241 ctgtcagact ccggtttctc cagtggcttc actctgaggg tcgtttcaac ctgcttgtcg
      301 tcaagcgtca gctttccttc cgcgttcacc cgataatcct tgaatgtctt gccctcgacc
      361 ggatgttgca tcgccacctt cacgccaaag tcgttatcgt tcgccaccgc cagcgttttg
      421 ctgtcgatca gcgccaaccc ttcagccttc tcctgctgcc agcccaagga gcgcagatca
      481 acaacctgcg ttttctgcgc aagggtaatg ccgcgctgtg acagggtttt ctcatcatca
      541 aactccggat actcacccgg cttgtcgaag cctgacaggt cgctcgcctt attgagatcg
      601 acctcgtaaa tcaggttacg cattctgttg tttttatctc ttccctgttc gatcagcagg
      661 atgtgctggt tatcgagcgc cacgatgtct ccgattttag cgtcgctgtt tttgctgtag
      721 gccgcgctgt cgataggata accgtacatc gcggtttttc cgcttgccgg atcgaagctc
      781 accagacgcg taaacagcgc ctttttcttg ctcttagcgt cgatatccag cgtactttgc
      841 acggcgacaa taatacgccc gtccggcata cgggtgaggc cttcaaagcc gcggttcgcc
      901 tgacgccatt tgaggatatt tggcaaaccg cccgcaatcg ctttttcccc ttccgccgcc
      961 tgcggaccgt ggattgccag gattttccct ttactgtcga tgttaatcag gaacgggcca
     1021 tactcatcgc acagccagta acctccttta ccatccggag tgatcccttc cgtatccagc
     1081 ccgcgattat cccccttcag tctatgtaac gtgtcgctga aggccacttc attggtggag
     1141 ccaatcacgt cgcttgccag cggcagaccg ttgatcgcgc ctttatcgtc atgcagagga
     1201 cggggatcga tggcctccgc tttgccgttt tgtacacgaa tcgtcatcag cagcggcgcg
     1261 aaatctggcg taacgaaaat tttagtttcg tttttcccct ctttcggtga atccgcgttc
     1321 ggaccgcgat cggtaatggt tgcgaatgtc agggcatcgc cctgtttacc ggtgaacagc
     1381 aggccagaac ctatcccaac cggaaggccg ttcgggaatg cactggcaaa tgcgccggca
     1441 tagttcacgt gcgtgccttc tggaaaactg acgacatagc gctcagccgt aggttgtgcc
     1501 gccagggcag agaaagagag cgtacaaccg attagcacgg gaataatttt tcttttcata
     1561 ttgttagcat ccataaagtg aagatcacac tacaccataa agaagaaacg agtcaggaaa
     1621 atgacatcga atggcaacat gcgcgggcct gaggccaaag aaatataaaa cactgttgtt
     1681 atttgtaaca acggaaacat ttacggaaat tatttgtctt tcttcgtgat caaaaatgct
     1741 gtgtcttgtt ggattggagt gatgagggct gcgtttagga aacgccctat attgtacggt
     1801 tgcattatcg cggtaggggt aatgcacgtg tcagtatgat gagctgacca cactcacgtg
     1861 ggtggactgg tataacaatc gacgattgct ggaaaggcag ggccatattc caccggcaga
     1921 agcagaaaaa gcttattatg cttccatcgg aaacgatgat ctggcagcct gagttcacag
     1981 ataaaacact ctccaggaaa cccggggcgg ttcaattgct ggataagatc ataacgatac
     2041 accgggttaa acaggtggtc actgcttcac tgaagctgcg tgattatgat ggtcaggtag
     2101 cagaagctat gcccatgata ccaaaatgac gaaagccgaa atgtctgaaa gcgtgtgtat
     2161 tacctgaaaa tatgaccggc tccggggtac gtaaccgatt caacaaagcc aatcgatatt
     2221 acaaaacgag agggactata tatttcgttt tcagataaga ctccttgaga gattatcttt
     2281 tatgtaaaat ctcaaatatc acctgatgta atgtcaatga taatattaag tgcgctctga
     2341 acaacatctt tcttggtgtg attaacatct gacaaaaaga acatcagctt ctttattatg
     2401 ttcttgttgt ttattatttc acgctcacag aataaagcgt tgactgcctc ttctatgaca
     2461 tttctctcgt aaaaaaattg agagttagct tcatataaaa tattggccaa tttttcacca
     2521 aaaaccatat tataataatc actgtgcatc tttctccccc agggttcagt caataatttt
     2581 accatgccta attatgacag gtgtaacaag ca
//
LOCUS       DAZGOB010000275         2609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGOB010000275 DAZGOB010000000
VERSION     DAZGOB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.277"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..346
                     /locus_tag="ACHLIF_005156"
     CDS             <1..346
                     /locus_tag="ACHLIF_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO9608959.1"
                     /translation="PPLNPPRGNRGVKKFDPLDIALPNWISVSLWREWVEFRQALRKP
                     IRTEQGANGAIRELEKFRQQGFSPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVS
                     LPDSAIPPGFRG"
     gene            353..1099
                     /locus_tag="ACHLIF_005157"
     CDS             353..1099
                     /locus_tag="ACHLIF_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9608960.1"
                     /translation="MKNIATGDVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEAQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIEK"
     gene            1122..1883
                     /locus_tag="ACHLIF_005158"
     CDS             1122..1883
                     /locus_tag="ACHLIF_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9608961.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPMQAELPEETTTATVVKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCMPGDNLPAEPKAASVAETDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIVDSSSRIVSEK"
     gene            1899..2321
                     /locus_tag="ACHLIF_005159"
     CDS             1899..2321
                     /locus_tag="ACHLIF_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9608962.1"
                     /translation="MAKVFTQEEREKIKEQVVELVRQSGRETLRQLEAKTGATRYLMS
                     ILARELVASGDVYNSGYGLFPSEQARKDWKNARKKLSRAKVKKTVVVDPDLIWSLPDG
                     EIRRYDRRQNIICRESRRSEVMQRVLAFYRGNFQEVME"
     gene            2318..>2609
                     /locus_tag="ACHLIF_005160"
     CDS             2318..>2609
                     /locus_tag="ACHLIF_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO9608963.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKVEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVA"
BASE COUNT          698 a          577 c          775 g          559 t
ORIGIN      
        1 tcccccccta aatccccccc gggggaatcg aggtgtcaaa aagtttgacc ctctggatat
       61 tgctttgccg aactggattt ctgtctcgct ttggcgtgag tgggttgaat ttcgccaggc
      121 attgcgaaaa ccgattcgaa cggagcaggg cgctaacggg gcgatacggg agctggaaaa
      181 attccgccag cagggttttt cacctgagca ggtgattcga cacagcatcg ccaatgaata
      241 ccagggcttg ttcgcgccga aaggtgttcg acctgagacg ttactccgac aggttaacac
      301 cgtctcgtta ccggatagtg cgatcccgcc aggcttcagg gggtaactga ccatgaaaaa
      361 tattgcgaca ggcgacgttc ttgaacgtat ccgcagactg gccccgtcac atgtaaccgc
      421 gccattcaag acggtagcgg agtggcgcga gtggcaactt tccgaaggcc agaaacgttg
      481 tgaggagatc aaccgtcaga atcgtcagtt gcgggtggaa aaaattctga atcgctctgg
      541 catccagcca ttgcaccgca aatgctcgtt ttcgaattac caggtgcaga acgaagcgca
      601 gcgatacgcg ttgagtcagg cgaaatccat cgctgatgaa ctgatgaccg ggtgtacaaa
      661 ttttgcgttc agcggaaaac ctggtaccgg gaagaaccac ttagcggcag ctatcgggaa
      721 tcgcctgctg aaagacggtc agacagtgat tgtggttacc gtggctgatg ttatgagtgc
      781 cctgcacgcc agctatgacg atgggcagtc aggcgaaaaa tttttgcggg aactatgcga
      841 agtggatctg ctggttcttg atgaaattgg cattcagcgc gagacgaaaa acgagcaggt
      901 ggtgctgcac cagattgttg atcgccggac agcgtcgatg cgcagcgtgg ggatgctgac
      961 aaacctgaac tatgaggcca tgaaaacatt gctcggcgag cggattatgg atcgcatgac
     1021 catgaacggc gggcgatggg tgaattttaa ctgggagagc tggcgtccga atgtcgtcca
     1081 gccaggaatt gagaagtaat ttttaccggg aggaaatttt tatggagact gtttttgacg
     1141 cactgaaagc aatgggaaaa gccacatcca tagaacttgc tgcgcgactt gatatcagtc
     1201 gtgaagaagt gctgaacgaa ctatgggaac tgaaaaaagc tggtttcgtt gataaaagcg
     1261 cgtacacctg gcgtgtggct gataacaacg ttcagcagga acagccaatg caggcagaac
     1321 tgccggaaga aaccaccacg gcaactgtag tgaaaatctc agagtgcgat ttaaccgcga
     1381 cgattgaaca acgcggacca caaacggcgg atgaactggc tacgctgttc ggtacaacat
     1441 cccgcaaagt tgcttcaacg ctggcaatgg caatcagcaa aggacgcctg attcgcgtaa
     1501 accagaacgg taaatttcgt tactgcatgc cgggcgataa tttaccagca gagccgaaag
     1561 ctgcatcggt agcggaaact gatggtaaag cctttcctca gccagccggt gttgcgttac
     1621 cagtacagga agcggcaaca caggaagata ttaaaacaga aactgtggcg gacattgtgc
     1681 agtcgctgcc atcgtttact gaaacgcaag cggatgacct agttttacca tcgctgcata
     1741 tggcaaaccg cgaactgcgt cgggcgaaaa atcatgtcca gaagtgggag cgagtctgcg
     1801 ccgcgctgcg ggagctgaac aagcaccggg atattgtccg acagattgtc gattcctcca
     1861 gtcgtattgt gtcggaaaag tgattgccgg aggcgcttat ggcaaaagtt tttacacagg
     1921 aagagcgtga aaaaattaag gagcaggttg ttgagttggt acgccagagt gggcgcgaga
     1981 cgttaaggca actggaggcc aagacaggtg cgacaagata tctgatgagc attcttgcca
     2041 gagagttggt tgccagtggc gatgtataca actctggcta tgggttattc ccgtctgaac
     2101 aggctcgtaa ggattggaaa aacgcccgca aaaaactatc cagggcaaag gtgaaaaaaa
     2161 cggttgtggt tgatcctgac cttatctggt cattaccaga cggagaaata cgccgctatg
     2221 acaggcgtca gaacataatt tgtcgtgaga gcaggaggag cgaagttatg cagcgtgtgc
     2281 tggcgttcta tcggggaaac tttcaggagg tgatggagtg agggtgagag tttatattgc
     2341 cggtccaatg acgggatatg aaaatttcaa ccgtgaggca tttcacaagg tggaagagga
     2401 actgaaacgg gaagggcata ccgtcttaaa cccggcagta cttccggacg ggctgacaca
     2461 gccgcactac atggatattt gcatggcaat gattcgttgt gtggatgcga tttacatgct
     2521 gaatggctgg cagcggtcag cgggcgctaa ggcagagctg gcactggcgg agaaactggg
     2581 gcatgcggtg atttatcagg aggtggctc
//
LOCUS       DAZGOB010000276         2576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGOB010000276 DAZGOB010000000
VERSION     DAZGOB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.278"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..119
                     /locus_tag="ACHLIF_005161"
     CDS             <1..119
                     /locus_tag="ACHLIF_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO9608964.1"
                     /translation="LLVWIIGRTFEPVIELYTDVTWESFSALHNKINPYKEN"
     gene            121..678
                     /locus_tag="ACHLIF_005162"
     CDS             121..678
                     /locus_tag="ACHLIF_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9608965.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            969..1124
                     /locus_tag="ACHLIF_005163"
     CDS             969..1124
                     /locus_tag="ACHLIF_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9608966.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1244..1588
                     /locus_tag="ACHLIF_005164"
     CDS             1244..1588
                     /locus_tag="ACHLIF_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO9608967.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1710..1982
                     /locus_tag="ACHLIF_005165"
     CDS             1710..1982
                     /locus_tag="ACHLIF_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608968.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            1984..>2576
                     /locus_tag="ACHLIF_005166"
     CDS             1984..>2576
                     /locus_tag="ACHLIF_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9608969.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSV"
BASE COUNT          660 a          592 c          709 g          615 t
ORIGIN      
        1 tattactggt ctggataatt ggaaggacat ttgaaccagt tattgagcta tataccgatg
       61 tgacatggga atcattcagc gcactgcaca ataaaattaa tccgtataag gaaaactgat
      121 atgagcacta ttaccagaga acgcgcggag attaaatcat acatcacagg cttcctgagc
      181 gactcggcgc acgataacaa gtcttcagac agcctgctgg ctaatgtgtt tcgtatcgcg
      241 ctggcatcac tggaagcaga gccgatagca atggtagtgc ctgatgaaat ggatttgctt
      301 acctgccatc tcgacggtgt aactaaaaca tatgctgatg gctggaacgc ctgccgcgtc
      361 gccatgcttc aggccggaaa ctttcgggaa aataagaatt cgtcaaccaa caattttcgg
      421 gaaatctcgg aaacgtcaac cagatctccg ataactctgg atggctggat aagctgtact
      481 gagcgaatgc ctgaaaagag ccagaacgtg cttatttcga tgaatatcga tagcgaggct
      541 gggccattaa tatattccgc acgctatctc ggaggcacgt tccggcgcgg aggtatagca
      601 gttagtccgg gtaatgatct taggcaagca acccactgga tgtcgctacc agaaccgccg
      661 caggaggtga atcaatgacc tggcctgaag cattcacaac ggtaggaatt gcgatggcgg
      721 tggcgctggt ggtgtattcg atttgccgct ggggataaaa acggtttgcg ggaaaaggat
      781 agttaagtag aattgcagcg ggtgcttgag gctatctgcc tcgggcatga acaccaacgg
      841 cagatagaga aaagccccag ttaacattac gcgtcctgca agacgcttaa cattaatctg
      901 aggctcaatc catgctgaac acatgtaggt tagcctctta cgtgccgaaa ggcaaggaga
      961 agcaggctat gaagcagcaa aaggcgatgt taatcgccct gatcgtcatc tgtttaaccg
     1021 tcatagtgac ggcactggta acgaggaaag acctctgcga ggtgcgaatc cgaaccggtc
     1081 agacggaggt cgctgtcttc gtagactacg aatctgagaa gtaagagacc aggcggggga
     1141 gtaatctccc gccacctctg atgtgtcagg catcctcaac gcacccgcgc tttaccatac
     1201 tgaaaatgct gtttgaatgt tcatctctga aagaggacta tgaatgaaaa aggtattgat
     1261 tgcagcactt atttccggtg tgtcttttgg cgcttttgca cagcagggtg gtttccaggg
     1321 gccagaagca gagcgttcaa cagtagcgca ggcaaaagaa ctgaaggatg atgcatgggt
     1381 tatccttgaa gggagcatcg ttaaaaaagt gggtgatgaa cgttatgagt ttcgtgacaa
     1441 tagcgggaca attgtcacgg atattgatga cagcgtatgg gccgggcaga atgtttctcc
     1501 gaaagacaaa gtaagaattg agggtgaaat tgataaagac ctgagcagtg ttgaagttga
     1561 tgtaaaggca ctgaaattat taaagtaacc gcccctgctt gttaagcccg tcttactgac
     1621 gggttttctg tttgtacatt ccggcgtatt gccttacaat tcgcgcagtc agcctgaaca
     1681 actgacacct gctgtcaccg gagaatccga tgacacaaca cataaaatcc cacaattctg
     1741 aagccgaccc ggaaattaag caggggaggc gttttcgtgc gcctcagtat ggctggtttc
     1801 actatctgtt ctgtacgatc gatgaggcag atatgcttca agaggcgtat ctgcgtcgcg
     1861 gtgtccgtgt ggagcggagt ctgaacgctg atcgtctgac ctggaccgtt tctgtatatc
     1921 ttcctgttcg tgcacatctg ccacggacac atgcctgcta ccgtcagcgc gtctggaggt
     1981 aatgtgcggg tattacttcg acctgttctg gttccggaac tcgggctggt ggtccttaag
     2041 cccggtcgtg aatcattgcc agtttttcat cgcggcaggg tgctggtgga gccggaaccg
     2101 aaaaacatgc gggcgctgcc atctggagcg gttcctgctg ttcgccagcc gctggcggaa
     2161 gataaatcac tgctgccatt tttcagcgat gagcgggtga ttcgtgcagc tggcggcgct
     2221 ggtgcactgt ctgactggtt attacgtcac gtgaaatcct gccagtggcc acacggcgat
     2281 tatcatcaca gcgaaaccgt tattcacagt tacggtgctg gcgcaatggt gttgtgctgg
     2341 cactgcgaca accagctgcg cgaccagacc tccgaatcac ttgagcaact tactcaacaa
     2401 aatctgacag cctggatgat tgacgtcata cgccatgtaa tgaatggcac gcaggagcgg
     2461 gaattatcgc tggctgaatt atcctggtgg gcagtctgca atcaggtggt ggacgcatta
     2521 cctgaggcag tatcgcgtcg ctctctggga ttaccggcgg aaaaaatccg ctccgt
//
LOCUS       DAZGOB010000277         2546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGOB010000277 DAZGOB010000000
VERSION     DAZGOB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.279"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            235..600
                     /locus_tag="ACHLIF_005167"
     CDS             235..600
                     /locus_tag="ACHLIF_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO9608970.1"
                     /translation="MSDMTISLPKEVNGHVICSTRIRHILAIRKSILMQIYMLFRQQA
                     GISALRQELEVKTPYRAMNHPVIGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAV
                     NNNGVTELFALLHTEEGCC"
     gene            complement(701..1030)
                     /gene="tmaR"
                     /locus_tag="ACHLIF_005168"
     CDS             complement(701..1030)
                     /gene="tmaR"
                     /locus_tag="ACHLIF_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO9608971.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1202..2260)
                     /gene="yeeA"
                     /locus_tag="ACHLIF_005169"
     CDS             complement(1202..2260)
                     /gene="yeeA"
                     /locus_tag="ACHLIF_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO9608972.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLIDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(2458..>2546)
                     /locus_tag="ACHLIF_005170"
     CDS             complement(2458..>2546)
                     /locus_tag="ACHLIF_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase inhibitor"
                     /protein_id="HFO9608973.1"
                     /translation="CFEVYLNNGAEDGYWDIEMYVAVQPKHH"
BASE COUNT          705 a          614 c          590 g          637 t
ORIGIN      
        1 cctactgatt gctaaggata aacaagtatt aaaggccaga ctgataagac cgcgactaag
       61 tgaacctgta tctgacatgg catagcttcg ctaatgtgtc atcatgatta ggactaaatc
      121 tgtgaattcc tttaggaagc acggacacag gccaatctga gtccatatgt atgattggaa
      181 tcttacacag atgacttttt gacacgggga cttggcaaac aggggcgacc atgtatgtca
      241 gatatgacaa tctcacttcc gaaagaagtc aacgggcacg ttatctgttc aaccagaatc
      301 cgacacattc tggctatacg aaagagcata cttatgcaaa tctatatgct tttccggcaa
      361 caagcaggaa tctcagcgct gaggcaggaa ttagaggtaa aaacgcccta ccgggccatg
      421 aatcacccgg tcattggagt agtaaccaaa gcagatttag ccagcatgga acacatatct
      481 ttggtgaaat gttggttacg ggaagctgga gcgcacaacg tgttagtaac cagtgcggtt
      541 aataacaatg gagttacaga actttttgcc ttgctgcata cagaagaagg ctgttgctaa
      601 tttcgatagg ttctcagatg agaatgatga cggtgataca cagataagaa tcaatgagca
      661 atgagggttg ccgggcaacc ctcattgaat aaaacaggaa ttacttcgct tcgccgtttt
      721 tcatttcgcc catagctttc agctttttgg agatatcgcg gcgttctttg gagagctcgg
      781 catttttgat gatgtaatcg tcaacgcgat cttcatagtc acctttcatg ctggcgatga
      841 tgccctggat tgcttcaacg ctcatcccgg gcttgatgta atcgctcagg ttgtccagca
      901 acaggacgcg cttctggttg tcacggatct ttttctcaac gtcctgaatt tcacgttgca
      961 gtttgttctt acgacggaac agacgaacaa attccagtac gtcctggaat gaaggcttgg
     1021 tagtttccat ttttataccc ctgataatgt gagagtcgga ttcgtttaag caaccgctat
     1081 tcgttagggc caacattact catagctgcc gcagatgaca atgcttttat cccttttcac
     1141 tatcataccc tttatccttg ctgaatcgaa gcagcagcaa gatgattctg aagttcagga
     1201 attatttgcg caaggcccgg caaatcagac tcgatagcaa ctcaagctga tgtgccgttt
     1261 ccatgttcag ccacacataa ccatagattg gtgtttccac aaccttcagg tcatggtggt
     1321 tattcagcaa ctgacgcagc tcttccacag catcgatcaa tttttccgta ttggcaaaaa
     1381 ccggctgcgg attaccttcg tacagcgcat gaacaaggct cagcagtatt tgctgcatca
     1441 tgtgctgggt atcacgaagt ttttgcgcgt tcaataacac gaaatggctg gggcgcgtgg
     1501 cccagtatgc attgatttgc aactccagca tacaaaccag attgcggtta atggtctgga
     1561 taccttcata tatcgatttt ggaatacgag tttctttgct ggcgggcgca atcagcccac
     1621 gcattttcac ggcatcggtc aggagttttt gtagatggct ttccagacgt gggcgttcga
     1681 gtaagttcgg tgagaatgca gattgataaa cccgattata ctcggtcaga cttttcgcca
     1741 gttgaatgcg ccagtggatg aacgcccgtt gtggccagat accggtaaac aacattgcca
     1801 gtaaagagcc gaggatcaca tcgccgcttc gccataacgc cgtatcaatt tcacctgtcg
     1861 gggaacccac aacaattgcc agcgtcaccc caatcaataa tccttgatac ggtttcttgc
     1921 ccagcgccag ccaaccgcaa agaaacatag ccgccgcgca ccagactaac atcagcggta
     1981 acgaaattaa ctccagttgc agagcgataa gacctaaaat cgaacccaac accgtaccgc
     2041 caatacgttc gaaggcgcga gggacaacgt taccccagaa cgagattggc cccataatca
     2101 ccaccatggt gaccagcggc caggtgcttt ccgggatagt aaacaggcgg ataatgagaa
     2161 aagtgagcag gaatgccagc gcgacccgag taccatgcac aatgcggtaa tggcggtata
     2221 cccggatttc aaacgggctt aatgacttat cggcacgcac gcagaaactc cgtaatgaag
     2281 attaaaagtg ctcataactc atctgtcggc cagaaggccc gcctctgaat tatccgacgg
     2341 acaaaccggg aacagtgtaa tttcccggca ggagcgaaaa aattgaaagg cgcatcttac
     2401 tcttttcgct ttcaaaaaaa gagtggtcat cgcgttaaca caccgccctg agatgaatta
     2461 gtgatgtttt ggctgcaccg caacatacat ttcgatatcc cagtacccat cttccgcgcc
     2521 attgttcaaa taaacctcga agcagg
//
LOCUS       DAZGOB010000278         2545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGOB010000278 DAZGOB010000000
VERSION     DAZGOB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.280"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(324..698)
                     /locus_tag="ACHLIF_005171"
     CDS             complement(324..698)
                     /locus_tag="ACHLIF_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608974.1"
                     /translation="MEFKDLPMPFQEMAANVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(721..939)
                     /gene="ydfC"
                     /locus_tag="ACHLIF_005172"
     CDS             complement(721..939)
                     /gene="ydfC"
                     /locus_tag="ACHLIF_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO9608975.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(969..1097)
                     /gene="ydfB"
                     /locus_tag="ACHLIF_005173"
     CDS             complement(969..1097)
                     /gene="ydfB"
                     /locus_tag="ACHLIF_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO9608976.1"
                     /translation="MDFNTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(1099..1254)
                     /locus_tag="ACHLIF_005174"
     CDS             complement(1099..1254)
                     /locus_tag="ACHLIF_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9608977.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTENGARRW
                     LERNSGE"
     gene            complement(1527..2243)
                     /locus_tag="ACHLIF_005175"
     CDS             complement(1527..2243)
                     /locus_tag="ACHLIF_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO9608978.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            2293..2508
                     /locus_tag="ACHLIF_005176"
     CDS             2293..2508
                     /locus_tag="ACHLIF_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9608979.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
BASE COUNT          641 a          570 c          563 g          771 t
ORIGIN      
        1 cgccaaacgt caccagaaaa ttgataacag agggcgttgc agcggggttg tcacttaagc
       61 gtatggtcaa cctgacaacc cggtgtcctc aacggggaag gaataacccc gccatactta
      121 ccgccgcgcc atttcgcgga ttgccacaac cggaagcgca cgatcgaatt aaatttaacg
      181 acgacctata cagagagact aacttcgccg ggcgctttcg tgttatgccc tgacttttca
      241 gggatatatc cttttcagta aactgtcagt gccggattct tatccgtgtc cggcgcacgc
      301 actctacctc acctgtgaat aaattaatga ttaattgata ttttgttgtt tgattcaact
      361 ttcccatcgg atgtgtgatg ctttaaatca caggaattaa tactgcttgc tgtaaaatga
      421 ttttcaaggg gagctattcg aatccctttc tttttcatta acaagccaaa tcctttatta
      481 atgatgtcca ttaattccag aaagtatttt tcatgtaaat cctggttatc agagagctgc
      541 ttctcttcgt acagcccgat aaaggctcgg cgtacgttac cagatatatt gtcgatggtt
      601 tctttttcta cggtactcag gtcaagagtc gccagttgag agcgaaccac attcgctgcc
      661 atttcctgga atggcattgg taaatcttta aattccatta ttagcctcgt tggttagcta
      721 ttaacgcggg tatgtaatca ttctggcaat gcttaatgcc gctgcttttt ccagcctggt
      781 gatatcctgc tccagagcgg acagattttc agcctgctta gccctggctt cattggccca
      841 tttcaggtcc tgcgcagcct taattttctg gtgcatccac tcataaagtt catcatcggt
      901 atagtctggc gcgatgatga cgggttctcg tttctgcata ctgattcctc gcggtgctgt
      961 ttcgcttatc agccgttaga ttttgccgaa ctggaaagcg cctgtttaaa ctcactgaag
     1021 ctgagggctt cttcgccttc ggcaaggcct tcgaagtatt cttcgtaagc cttttccatg
     1081 attgtgttga aatccatatc actcacctga gtttctttcc agccagcgac gggcaccatt
     1141 ttcggtttta aacgttttgc ttctggtata cgtcatcgcg gtgaacgtac cgtcctggtt
     1201 ggggaacacg ccacatacca gagattcgct gttgccaaga tcgatagtat ccatgctgac
     1261 ctcatttccc cttaacgccg gggtagcgga acaaaaacct gctgcatagt tattaaagtt
     1321 gaaccctgcc gtcatgttct tacgcctcgg gctggctact taacccctga ccactgccgg
     1381 gtaactcgaa gtatttccct gcgttctgtg gggcggggtg ggttggtatg ttgttaaggt
     1441 aacaagagtt acctttcgag tcaatacaat gttgcaaaag gtacatttga gggcataaaa
     1501 aacccgcaat gaatgcgggt tctgactcag tctaagtatt gatgtatttg tgaaacttta
     1561 cctttaatgg tgtaaccacc attcagttcg atgggtttgt aaagcggatt cagtgacaac
     1621 agatagatgt ttggtccgtc aatcgcaact ttttttagtg ttacgtttgg cgttccttcc
     1681 aattggatta agattatttt tcccaccagt tctctaatgt tacttgagca tggtgtgatc
     1741 agcacggtag atccgtcggg gatggttggg aggccgttag agtttgtcat cgcatctccc
     1801 tcaacatgca ataaaaaaga gttttcagcg gtttttgtca tgacatcaac ccagttctta
     1861 ataccaggaa tcttggttac tggacaactc atatcccaat aaccagcctg ttcccacgtt
     1921 aaaacgggca accgggcgat gttgtcacta atgtaagggt actgattcag acgcagatca
     1981 tcggttttat cgtgaccgtc ctttccataa agaatccatt caggagattt ggaaagcaat
     2041 tttgacagta gatacaaatt ctcaccgtca ggttttgaag agccattttc ccattttgtt
     2101 acggatacac gagatatgcc gattgctttc gcaacctgct gttgggttaa tccaacgtct
     2161 tttcgacgat tccgaatacg ttcgctgata gtgtttttca tgtaaccaat gttactacca
     2221 agtgatgttg ctatggttga cattgttatg taactattgt tacccttctg ctcgaaataa
     2281 caggagagtt ttatgttcaa agatgatgtt ctgcgctatt tcaaaaaaaa gcgactagta
     2341 gctgaggctc ttggaatttc acatgtggct gttgtgcggt ggaaagcagt tattcccaaa
     2401 cttcgcgcaa tggaactgga tgaaattact aacggtgaat tgaaatacaa cccagaactt
     2461 tacaagaagc aggatagcac ctcgaacgaa ggaaagaatg attcatgaaa atcaagcatg
     2521 aacacatccg catggcgatg aatgt
//
LOCUS       DAZGOB010000279         2507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGOB010000279 DAZGOB010000000
VERSION     DAZGOB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.281"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..971
                     /locus_tag="ACHLIF_005177"
     CDS             <1..971
                     /locus_tag="ACHLIF_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9608980.1"
                     /translation="NTIEEKIHSLLKKWADEIKVDRSDYYAKTISVVDSIDNDSQIEN
                     VFNLLDSLYVESVDTLTFKYQSIIKGLDRLFEGINLDSAFSLSEEERSYFEEKAKSLN
                     ALAQLGISVEIISHELEEMDSMVTRGLNSLPTSVKEHPGFSLALNAHRSLTQQIRFLS
                     PLKISGYQSRQRITGKNIMDYVLKFFGERFERQRITIEFSEEFKQIAITDIPLRIYPV
                     FTNIINNAMYWVSLSNNRLIKIGFVNSLVIIANSGPAIDTDDIPRLFELFYSKRANGH
                     GVGLYLCRENLAVAHHKIWYSEPDEGDNYLIKDGANFVIQFNGVEF"
     gene            973..>2507
                     /locus_tag="ACHLIF_005178"
     CDS             973..>2507
                     /locus_tag="ACHLIF_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFO9608981.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIRKSDLIIVDYHLDNNAPD
                     KTLKLLQDLKDSDHLNMIVIYTRENLETVWMQISSTLKGALDINSLIIDYDNEDVQSY
                     WEDVVLPNLNDNGNKALTRDEIIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMI
                     AEYAVSRNAIISSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTL
                     NDSLIEWKPSYYQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWLNEILKSDSPDIRC
                     RNIDFVFGNLSEELYQRLKNNNTLDEFIKSVFDSYSNEYANSGVAALLQYCSSKMDLP
                     SNNDTYHEMYHALNMNLSSKNFEDGHISTGTIFFDTESNKWYLCVSAACDLVPTQGND
                     PHHVRLSPHRLIKVLELFNASQSKALPFAEHSKYIYVMHKNQRKYLSIFEGDKTLPVV
                     DYMV"
BASE COUNT          855 a          377 c          470 g          805 t
ORIGIN      
        1 ataacacgat agaggagaag atacattctc ttttaaaaaa atgggccgat gaaataaagg
       61 ttgatcgaag tgattattat gctaaaacta tatcagttgt tgattcaata gataatgatt
      121 cacaaattga aaatgtgttt aatttgctcg atagtttata tgttgagtca gttgataccc
      181 taactttcaa atatcaatca ataataaaag gtctcgatag attatttgaa ggtataaact
      241 tagattcagc attctcatta tctgaagaag aacgctcata ttttgaagaa aaagctaaaa
      301 gtttaaacgc gcttgcacag ttaggtatta gtgttgagat aatatctcat gaacttgaag
      361 aaatggattc tatggtaacc agaggactaa actctcttcc tacttctgta aaagaacacc
      421 ctggtttttc attggcgtta aatgctcaca gatcgcttac tcaacaaata cgtttcttat
      481 cacctttgaa aatatcaggt tatcaatcca ggcagagaat aactggaaaa aatatcatgg
      541 attatgtcct gaagttcttt ggggagcgtt tcgaacggca acgaataact attgaattta
      601 gtgaagagtt taagcaaatc gcaataacag atataccatt aaggatctat cctgttttta
      661 ctaatattat caacaatgca atgtattggg tcagtctgtc aaataatagg ctcataaaga
      721 ttggttttgt gaattctttg gttatcatag caaattctgg tccggcaatt gataccgatg
      781 atatcccgcg actatttgaa ctattttata gcaaaagagc aaatggacat ggggtaggtc
      841 tgtatctatg tcgagaaaac cttgctgttg cacatcataa aatatggtat tcagaacctg
      901 atgaaggcga taactattta ataaaagatg gcgctaattt tgtgatccag ttcaatggag
      961 tggagttcta atatgacagt ggcaaattat aattctcttg tccagaaaac tttctgcgaa
     1021 aatgcaattc gttccgttgt catgattgat gacgattttc tgacgtattc tgaatcaatc
     1081 agggcgttga ataacgaagt tgatttagac tacaacaaaa ttgactcatc taaacgagcc
     1141 gctactcttg agagcttttt tcaatctaaa aatatgattt gtgatgttga caatggttct
     1201 gttaatttcg atgtggatcg gattagaaaa tcagatctta ttattgtaga ttatcatctt
     1261 gataataatg cacctgataa aacacttaaa ctattacaag atttgaaaga ctccgatcat
     1321 ttaaatatga ttgtaatata tactagagag aatttagaaa cggtttggat gcagatatca
     1381 tcgactctca aaggtgctct ggatatcaac agcttgatca ttgactacga taatgaagat
     1441 gtccaaagtt attgggaaga cgttgtatta ccgaacttaa atgataatgg taataaagct
     1501 ctcacaagag atgaaataat agcctatatt aaagacagta agccttgtag aagaattaaa
     1561 agattaatac atgatgatgc tgtgttggag gatcaaaagg ataaaaactt cattgcaaaa
     1621 atgattgcag aatatgctgt gtctagaaat gcaattattt ctagcaacac atctggcaat
     1681 gtcattcggg gtgatgaaag cggagtaaaa tggattcaat gtggtaatat ctttgtctcc
     1741 ctatttcata aggttcaaga tgatcatgaa aacgatggag ataggatttg gcaaactctc
     1801 aatgattctc tcattgaatg gaaaccatct tattatcagt taataaaatc tgaaattcag
     1861 aatgcaatcg aagctgaggc tttatctttt gtaaatcatt tggctaacga tcattacggt
     1921 caagctgcgt ggttaaatga gatattaaaa tcagactcgc ctgatattag atgtagaaat
     1981 attgactttg tatttggtaa tttatcagaa gagctttatc aaagacttaa aaataataat
     2041 acgctggatg aatttatcaa aagtgttttt gatagctatt caaatgaata cgctaacagc
     2101 ggagttgctg cattgctcca atattgctct tcaaaaatgg atctgccatc aaataatgat
     2161 acttatcacg aaatgtatca tgctttaaat atgaatttgt cttcaaagaa ttttgaagat
     2221 ggtcatattt ctactggcac tattttcttt gatacagagt cgaacaaatg gtatttatgt
     2281 gtatctgcgg catgtgattt ggttcctact cagggtaacg accctcacca tgtaagatta
     2341 agtccgcaca ggctcattaa agttctggag ctttttaacg ccagtcagag taaagcattg
     2401 ccatttgctg aacattcgaa atatatatat gtaatgcata aaaatcaaag aaaatatctc
     2461 tctattttcg aaggggataa aacgcttcct gttgttgatt atatggt
//
LOCUS       DAZGOB010000280         2507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGOB010000280 DAZGOB010000000
VERSION     DAZGOB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.282"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..373
                     /locus_tag="ACHLIF_005179"
     CDS             158..373
                     /locus_tag="ACHLIF_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9608982.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            373..870
                     /gene="rrrD"
                     /locus_tag="ACHLIF_005180"
     CDS             373..870
                     /gene="rrrD"
                     /locus_tag="ACHLIF_005180"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO9608983.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            867..1304
                     /locus_tag="ACHLIF_005181"
     CDS             867..1304
                     /locus_tag="ACHLIF_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9608984.1"
                     /translation="MSRVTVIISALVICIIACQSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTSDAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            1454..2071
                     /locus_tag="ACHLIF_005182"
     CDS             1454..2071
                     /locus_tag="ACHLIF_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9608985.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(2259..2453)
                     /gene="ylcI"
                     /locus_tag="ACHLIF_005183"
     CDS             complement(2259..2453)
                     /gene="ylcI"
                     /locus_tag="ACHLIF_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9608986.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
BASE COUNT          647 a          561 c          657 g          642 t
ORIGIN      
        1 tttataacct gaatcagggg aggccggaat gttatctggc atttttagca gagcctgaat
       61 gccataatca cggctcccgg cgttggccgt cagtgggtga cactggcggc ttttttgttt
      121 ttctttactt tcattttctg tcggcggtga cggagacata catcagatgg aaaaaatcac
      181 aacaggtgtg tcatacacca cgtcagcggt ggggacggga tactggttac tgcagctgct
      241 ggacaaagtc tctccgtccc agtgggtggc aataggtgtg ctgggaagtc tgctgtttgg
      301 cctgctgacg tatctgacaa atctttattt caagattaaa gaagataagc gtaaggctgc
      361 gagaggtgaa taatgtcgcc gtcattacgc aaggctgttg cagctgctat tggtggtggg
      421 gctgttgcca tagcgtctgt gctcatcact ggtccaggtg gtaacgatgg tctggaaggt
      481 gtcagctaca taccatacaa agatatcgtt ggcgtatgga ctgtatgtca cggacacacc
      541 ggaaaagaca tcatgcccgg taaaacgtat accgaagcag aatgcaaagc cctcctgaat
      601 aaagaccttg ccacggtcgc cagacaaatt aacccgtaca tcaacgtcga tataccggaa
      661 acaacgcgcg gcgctcttta ctcgttcgtt tacaacgtgg gcgctggcaa tttcagaaca
      721 tcgacgcttc ttcgcaaaat aaaccagggc gatatcaaag gcgcatgtga tcagctacgg
      781 cgctggacat acgctggcgg taagcaatgg aaagggctga tgactcgccg cgagattgag
      841 cgtgaagtct gtttgtgggg gcaacaatga gcagggtaac cgttattatc tccgctctgg
      901 ttatctgcat tatcgcctgc cagtcatggg ctgttaatca ttaccgtgat aacgccatcg
      961 cctacaaaga gcagcgcgat aaagccacat ccatcatcgc tgatatgcag aagcggcaac
     1021 gtgatgtagc agaacttgac gccagataca caaaggagct tgctgatgct aatgcgacta
     1081 tcgaaagtct ccgtgctgat gtttctgctg ggcgtaagcg cctgcaagtc tccgccacct
     1141 gtgcaaagtc aacgaccgga gccagcagca tgggcgatgg agaaagccca ggacttacat
     1201 cagatgctga actcaattat taccgtctcc gaggtggaat cgacaagata accgcgcagg
     1261 ttaactacct gcaggaatac atcaggacgc agtgcttaaa ataattttaa tttcactgaa
     1321 atttaacaag tgactttcag gaaaatgcct cgcagatgcg gggcattttt gtaccggtat
     1381 ttcaccgcgc accgcagcgc acaataaaca ccgaacctga ccctttggaa tgggcctttg
     1441 aggataccag ttagtgctgg cgagcctcgg tgggctggtt tcctgtgcgg caaaggttca
     1501 tttcaaagaa gcaggcaacg ccatgaatga attaattgcg aatcatgact tcgactttcg
     1561 ccagttagtt accgcagcag aaggtcaacc ggtaactgac accttccaga ttgccagggc
     1621 atttggtaaa cgccatcagc atgtgattag ggctattaaa tgtttgagat gttctgagga
     1681 attctcgaca acccattttt gggccgtcga gaaaatcaat gacttaggta tttttgacaa
     1741 gaaacagatt tactaccgca tggactttag tggcttcgtt atgctggtta tgggatttaa
     1801 cggggcaaaa gccgatgctg ttaaagaagc ctatatcaat gcgtttaact ggatgtcagc
     1861 agaactccgt aagtacagcg aaagttatga agcagaacgt aacgccgtaa tgctggagta
     1921 catgaaagag aaggatgtcg ccagcatgtc aggccgtctg ctcaatcgct gggggagaac
     1981 gaaaaaacct caattgcttg caaagctgga acgtctggag agacagggac agtttttatt
     2041 accgggattc gataaaggta ttcaagcctg acacattatg cgctgtatcg tcgccgtatt
     2101 cccgcattaa ccatgaccgt agcccgacgg ggaattcctt ctgcgtgagt gtgcgggaat
     2161 aatcaaaaac gatgcacacc gggttttact gtgctgacag acgcagggtt accctcatag
     2221 tcgcttttcc ggtgcgatgg tggaagaaac cgggatgttc atccatcatc actttggatt
     2281 gatgtatatg ctctcttttc tgacgttagt ctccgacggc aggcttcaat gacccaggct
     2341 gagaaattcc cagacccttt ttgctcaaga gcgatgttaa tttgttcaat catttggtta
     2401 ggaaagcgga tgttgcgggt tgttgttctg cgggttctgt tcttcgttga catgaggttg
     2461 ccctgtattc agtgtcgctg atttgtattg tctgaagttg tttttac
//
LOCUS       DAZGOB010000281         2490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGOB010000281 DAZGOB010000000
VERSION     DAZGOB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.283"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1270..2142
                     /locus_tag="ACHLIF_005184"
     CDS             1270..2142
                     /locus_tag="ACHLIF_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9608987.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKITRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT          727 a          633 c          513 g          617 t
ORIGIN      
        1 actcagggga aactcatcac aaggctgacc gaatgacata gtaacctcac tatacacaga
       61 tataaaagca ctgcaattct ggctattaat agcaaaaata gcagtcaaat caatatgagg
      121 taaaattttt tgttgattta atggttaaat aataaccacg gtgtattttg ctgctgaaga
      181 aagacaaaat gaatctgtgt gtcccctcct tcccttcgga gcacatccac tattcaaatt
      241 ttacacaacc acgtacgaac aaaaagcatc atcccgggta ttgcagatac cggtttcctg
      301 ccgggaagaa tcataaacag aacaccggga ataacgcctt caggaatcac cccccctcct
      361 gaagaagaat aaaataaaga agactacagt tcctgcactg aatgctgagt acaatattct
      421 atggacagga tgacattttt ataaataaca aaaaccattt aatactgata catcaataaa
      481 atacttcttc cctgctgaca cagataaaat gcaccatttt ttcttcaaat acttctgtat
      541 aatcgccagc caatgatgag aatggattaa aataacactt cttcgacaaa cacaaaaaca
      601 gccttcattt tacatcatca tccagtttca gttaaccccg gacgagcgac agtgtttcag
      661 tacaggaatt aaaagccagc ttttgctcag taaaacacac tcaactaata atatctcagc
      721 agatattatc ctgaccagga cacctcacat tactgaattc tcttccgaca gtatcactgc
      781 acaaataaaa aagaacggga taaatattca gaccggaaga atggtgtcac aaaagcattt
      841 aatcattacg catgaccagg aagggaaaca cttaaccaca tggtatcaac agatatcatt
      901 acgtgtgcac tcttataaaa aacacacaga agaagtaaga taattataaa cgtaaattta
      961 atatctccca ctgacatctc ccccacaata cactaaaaaa tactcacagc agtaaacaca
     1021 gacacacctg ataatcatac agccgataaa tattttcccc tttacggaga aatcagcctg
     1081 ctgtttaaca ataacggcgt attttcatgg aaggagatca cccagtaacg gataagtcct
     1141 gatctccttc atgttaatac gcaacaatcc catcgattca aaacaatttc agacagatat
     1201 tatctccgtg aatcccccgc cacctttccg gtgcgcgggg ttttgtcttt tttcaccggg
     1261 aatacatgta tgaatccgtc tgatgccatt gaggcaattg aaaaaccgct ctcctccctg
     1321 ccttactcac tttcccgtca catcctggaa catctgcgca aaatcacccg tcacgaaccc
     1381 gtgattggca ttatgggtaa aagcggggcc ggtaaatcct cactctgtaa tgcactgttt
     1441 cagggggagg tcaccccggt cagtgatgtt cacgccggca cccgggaagt acggcgcttc
     1501 cgtctgagtg gccacggtca cagcatggtt atcactgacc tgcccggggt gggcgagagc
     1561 cgggacaggg atgcagagta tgaggccctg taccgtgaca ttctgcctga acttgacctg
     1621 gtactgtggc tgattaaagc cgatgaccgt gccctgtctg tggatgagta tttctggcga
     1681 cacatcctgc agtgcggaca tcagcaggtg ctgtttgtgg tgacgcaggc cgacaaaacg
     1741 gagccctgcc atgaatggga tatggccggt attcagcctt ctcctgcaca ggcacagaat
     1801 attcgcgaaa aaacggaggc ggtattccgt ctgttccggc ccgtacatcc ggttgtggcc
     1861 gtatcggccc gcaccggctg ggaactggat acgctggtca gtgcgctcat gacagcgctt
     1921 cccgaccatg ccgccagtcc cctgatgacc cgactgcagg acgagctgcg cacggaatct
     1981 gtccgcgctc aggcccgtga acagtttacc ggtgcggtgg accggatatt tgacacggcg
     2041 gagagcgtct gtgttgcctc tgttgtccgt acggccctgc gcgctgttcg tgacacggtg
     2101 gtctctgttg cccgtgcggt atggaactgg attttcttct gaacctgtcg tgactgatgc
     2161 cctccctgac tctgagtctg ctcacaaaag cactgttttc gttactgtct ctcttgtccg
     2221 tgcaatagct caataataga ataaaacgat caatatctat tttatcgatc gtttatatcg
     2281 atcgataagc taataataac ctttgtcagt aacatgcaca gatacgtaca gaaagacatt
     2341 cagggaacaa cagaaccaca attcagaaac tcccacagcc ggacctccgg cactgtaacc
     2401 ctttacctgc cggtatccac gtttgtgggt accggctttt ttattcaccc tcaatctaag
     2461 gaaaagctga tgaaacgaca tctgaatacc
//
LOCUS       DAZGOB010000282         2401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGOB010000282 DAZGOB010000000
VERSION     DAZGOB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.284"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..452)
                     /locus_tag="ACHLIF_005185"
     CDS             complement(<1..452)
                     /locus_tag="ACHLIF_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9608988.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLQVTDNKGKTHDVLTGSDDG"
     gene            complement(523..>2401)
                     /locus_tag="ACHLIF_005186"
     CDS             complement(523..>2401)
                     /locus_tag="ACHLIF_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9608989.1"
                     /translation="PDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTF
                     AITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWD
                     TPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQ
                     QGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQ
                     VETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLD
                     FFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSNEITQTVNKKLEDQSAAIQ
                     QIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAM
                     INPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADI
                     SGSVNANSGALNNVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNG
                     TVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIAS
                     RETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISI
                     R"
BASE COUNT          513 a          704 c          581 g          603 t
ORIGIN      
        1 ccgtcatcac ttccggtcag cacatcatgc gttttcccct tgttgtcggt cacctgcaga
       61 taatctccgg agaaagtcga cacacggctg taagccacgc ccgccatcgc atacgcgctg
      121 aaccattcat tcacgcgcac agacggcccc gccattacgc tgaaccagcg gttacgaacg
      181 gaatcttcat gccagcgggt atcgctgtaa tgggtcagct ggcgattctt gtctcctgca
      241 tagctgaacg acgtcaccat ccccagtgtg tccgtaaact cataacggta tttcacgtta
      301 atcccgttca gttcatcgct gccaggaacg ttcgtcgaga catgaagata ccccgcgctc
      361 agcgtggact gatgttcaga cgcccatgca ggcgcaccgg atacggccag acagatggct
      421 gcggacaaaa tggcggcata aagtttacgc ataattacct ctcgcttttc tgcaataaaa
      481 aaggcgtcat tcctgacgcc ctttattggg gttataaata tttcaacgaa tactgatgcc
      541 ggaagcagct tttttggtca caatcaccgt acagtcggtg atattgcctg ccccctgatt
      601 gcctttctgg aaaatcttaa actccagagt gacgctacca ccaccactag gcatatcaat
      661 aactgcactg taactaccgg gaatggcccc tttagtttct ctggatgcga ttaatacgcc
      721 gtttttgcga acttcaaaac cataccccgt gtatcgcgtg cctcctgggt tatttccgct
      781 ccctggatcg tcatacgcta taccgttaaa aataatgggc ggaataataa tctggcggtc
      841 aaagttatga tcatcgctga tggtgactgt aaccgtaccg tttggtgttt ccgtgttacc
      901 ccacgtaccg acttttttcg ggaaggcttt tgatacagct ttaacgaaat cccctctgac
      961 ctgggtcgcc tccagcatgc ccttaatcgt acagttctgg ttaatcgtga cattgttgag
     1021 cgctcctgag ttcgcattca cactgccact gatatccgca tttttcgccg tcagtcgccc
     1081 gtctggtgtc agggaaaatg ccggaggatt accgccgctg gtaatggtgg gagccgtcag
     1141 atatttcagg aacacttcat tcatgaatat ctgatcgccc tgaccaacaa acatcggctt
     1201 tgtgttgcca ttcgcaggat taatcatcgc aatcctgtct gctgccagca gcacctgact
     1261 ctgcatgccg tcgggggtgt tctcaatacc ggcaccgata cccgcaatat aaaggcgtcc
     1321 gtcctgcatc tgctgcagct tcactgccca catgctgttc aggttattat ttgtatcaac
     1381 ctgaaccttc tgtatctgct ggatcgctgc actctggtct tccagtttct tattgacggt
     1441 ctgtgttatt tcattgctga catccgttat ggacgtcctg atttcagtca ggtcaggcgc
     1501 aagctgaccg ttatcaatct gcgtccacag ctcctgagcc agatgggttt tccctatctc
     1561 gcctttgaaa aaatccagat agcctgatgc atcatcactc ggctgaccga cagcctctac
     1621 aaatgccgat ttgccaacgg tgttcacact gcggatataa aagtaataat catggcccgg
     1681 tttgatattg atactggcag ctatccagta cagcgccgtg ccaagatagc gggctgtggt
     1741 ttcaacctgc ctgatatcag caatccgctt ttccgagaac cagaactcaa actgtaccgt
     1801 cggatcataa accgcaagat gcggcgtggc ggttatctga aaatagcccg gtgtcagctc
     1861 aatccgagac ggcgctgccg gtgcggcaat ccggaacgat accgatgccg gatcgccctg
     1921 ctgtccccac gcatttaccg cccggactgt caacctgtag ttccccggcg ccagttgcgt
     1981 gaagcggtat gtggtttctg tcgtcctggc cgtgctgacc agccgctcac tgccgtcatc
     2041 cgctgccacg gtcaggcgaa gcaggaagct cacgcccttc accaccttcg gcgtgtccca
     2101 gcgcgccagc acctgatatt ccccgctgtc tgcggtgact tcggcggtca ggtgctgcac
     2161 cgctggcggc gtgacaccat tcaccgtgcc gctctggtcg ccgtcaaagt gcgccccgtt
     2221 atccacgatg gcttcttttt ccggcacgtg ctgcaccgcc gtgatggcaa aggtgccgtc
     2281 cgtgttttcc cggatggaga cacagcggaa caggcgacga cgcagtgacg gcagggagag
     2341 tccccataca ccgtatgtct ccacaccatc aggcagggtg ctgacctgta tccggtccgg
     2401 c
//
LOCUS       DAZGOB010000283         2399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGOB010000283 DAZGOB010000000
VERSION     DAZGOB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.285"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..192
                     /gene="etgA"
                     /locus_tag="ACHLIF_005187"
     CDS             <1..192
                     /gene="etgA"
                     /locus_tag="ACHLIF_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO9608990.1"
                     /translation="INISVAGYILASNIKSRGNTWDAVGAYNAGYFNTPNAVELRRQY
                     AMKIYKTYTKLKNNEQIID"
     gene            complement(189..1226)
                     /gene="escU"
                     /locus_tag="ACHLIF_005188"
     CDS             complement(189..1226)
                     /gene="escU"
                     /locus_tag="ACHLIF_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO9608991.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GASFFVEVVELVNTTIDSLNRPFLYAIREILGAVLNIFLLYILPISLIVFVGTVTTGV
                     SQTGFIFAVEKIKPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVIITLIFYFMGHSY
                     ANEFANFTRLNAYQTLVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKKMKMSKD
                     EVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICLYYKLGE
                     TPLPLVIETGKDAKALQIIKLAELYDIPVIEDITLARSLYKNIHKGQYITEDFFEPVA
                     QLIRIAIDLDY"
     gene            complement(1219..1995)
                     /gene="escT"
                     /locus_tag="ACHLIF_005189"
     CDS             complement(1219..1995)
                     /gene="escT"
                     /locus_tag="ACHLIF_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO9608992.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIVLKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFITVIFVIHGGIQSVLDKLYLSYEILPLQAD
                     IAFNRALVDFLFSLWDSFIKLMLSFSIPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            complement(1995..2264)
                     /gene="escS"
                     /locus_tag="ACHLIF_005190"
     CDS             complement(1995..2264)
                     /gene="escS"
                     /locus_tag="ACHLIF_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO9608993.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(2264..>2399)
                     /locus_tag="ACHLIF_005191"
     CDS             complement(2264..>2399)
                     /locus_tag="ACHLIF_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO9608994.1"
                     /translation="ISNILLALGMMMVSPVTISIPFKILLFILVGGWQKLFEFLLVVN
                     "
BASE COUNT          856 a          444 c          387 g          712 t
ORIGIN      
        1 ataaacatat ctgttgctgg ttatatctta gcctctaaca taaaatccag ggggaataca
       61 tgggatgcag ttggtgcgta taatgcaggc tactttaaca caccaaatgc agtagaactc
      121 agaagacaat atgcaatgaa aatatataaa acatacacta aattaaaaaa caatgaacaa
      181 attatcgatt aataatcaag atctattgct atacgaatca attgtgctac aggttcaaaa
      241 aagtcttctg ttatgtattg tcctttatgt atgttcttat aaagacttct tgccaaagta
      301 atatcttcaa tcactggaat atcatagagt tcagccagtt taatgatctg tagagctttg
      361 gcatcttttc ctgtttcaat aactaaaggc aatggagtct ccccgagttt atagtatagg
      421 caaatcgcaa tgtgagtcgg gtttttgacg atcacggttg attttttgat gttattagcc
      481 agacttccac tttgtatctc agaatgaagg cgacggcgct cccctttaat ttcagggtta
      541 ccatcagtat ccttggcttc tcgtttcacc tcatctttac tcattttcat tttcttcagt
      601 ccctcatgct tctggaacca gaaatcaaat actgaaaaga gtagatatcc gaataacacg
      661 cctttccata aaagaaaaac aaaaaaggca acaacgacaa gagtttgata tgcgttcagt
      721 cttgtgaaat ttgcaaactc atttgcatat gaatgcccca taaagtaaaa aatgagagta
      781 attatcacta acttaaatac tgacttaagc agctcaaaaa tactctttac agaaaaaata
      841 tttttcaggt tattttttac actaatcttc tgagccgatg gttttatttt ttcaaccgca
      901 aagatgaatc ctgtttgtga tacaccagtt gtaacagttc caacaaagac aatcaaagaa
      961 attggcaaaa tatacaataa aaatatgttt aataccgcac ctaatatttc acgaatggca
     1021 tacaaaaacg gtctatttag cgagtctata gtcgtattaa ctaactcaac aacctcaacg
     1081 aaaaaactcg caccatataa agaaaaaaat gaaaataaga ttaatgactg cactgcagcc
     1141 attacctctt cactttttgt tacatcgccc ttctttttta gatcccttag ttttttgggt
     1201 gtgggctttt ctgttttttc actcattaat catgctcggt aacgatctgt caattatgtc
     1261 agaatgaata tttgccgtaa taaaatcagg gaaaacatga ataactaata acaatataaa
     1321 gattgctatc aaacttttta ccggcagtga taatgtgaat acgtttagct gaggcgctgt
     1381 tttattgaga aacccaaacc ccatatcaca taagaagata ccgataatca tgggaattga
     1441 aaatgataac atcagtttaa taaatgaatc ccatagcgaa aataaaaaat ctactaaagc
     1501 acgattgaat gcaatatcgg cttgtaatgg taatatctcg taggataaat acaacttatc
     1561 cagaacgctt tgtatcccac catgaataac aaagatcaca gtgataaatt ggtataaaat
     1621 aacaccagtg atagaagatg aatcacttat agacgggtta aaaattgaag atattgttga
     1681 acctcttaaa gtatcaataa tctgcccagc cgcatctatt gcccaaaaaa gaatagtaaa
     1741 tgataaccca atgaaaaagc ctatacttat ttcttttagc actatgcctg tcaactgaaa
     1801 aattccggat ggtaattttt ccgaaaatgt atagttctct actataattg gaagtgtaaa
     1861 cgcaatcata attgagttgc gtataaagtt actgagcaaa acacctgttg aaaatattgg
     1921 tagaataata aacatcccca gcggcctgag tatacaaaaa aatgatgata ctatgaccgt
     1981 cattatttca ttcattagcc attcaccttc ggaatcattt caaatattat cgacgagaag
     2041 ttgatgattg ttgttcccat ccagtgataa gtcagggcaa gcgtagcaaa cactgctatt
     2101 atttttagca aaaaaggcaa tgtttgatcc tgtaactgcg ttatagcctg aaccaggcta
     2161 ataataatgc cgacaacaga ggccgctatg actgtaggca gtgagaggat aaatattatc
     2221 caaagcgttt gcacacataa ttgaacaaaa tatccagttt ccatcaattc accaccaaca
     2281 gaaattcgaa taatttttgc cagcctccaa caagaatgaa taacaagatc ttaaaaggaa
     2341 tcgaaattgt tactggcgat accatcatca tacccaaggc caataagata ttggaaatg
//
LOCUS       DAZGOB010000284         2391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGOB010000284 DAZGOB010000000
VERSION     DAZGOB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.286"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..270
                     /locus_tag="ACHLIF_005192"
     CDS             <1..270
                     /locus_tag="ACHLIF_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9608995.1"
                     /translation="NIIEIGYNIINTDLVIGEKIHFRTLSNRSLYFNLTQPPLIATIK
                     DDVFCTLHDYYNHNNETKSTIDNYISKIKDNHNTPWLLNNENVQE"
     gene            complement(1141..1281)
                     /locus_tag="ACHLIF_005193"
     CDS             complement(1141..1281)
                     /locus_tag="ACHLIF_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9608996.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1583..1846)
                     /locus_tag="ACHLIF_005194"
     CDS             complement(1583..1846)
                     /locus_tag="ACHLIF_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO9608997.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            2269..>2391
                     /locus_tag="ACHLIF_005195"
     CDS             2269..>2391
                     /locus_tag="ACHLIF_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO9608998.1"
                     /translation="MKTKKVMMIALVSSALALSGCGAMGTAIKKRNLEVKTQMSE"
BASE COUNT          741 a          552 c          439 g          659 t
ORIGIN      
        1 aatataattg aaataggcta caatataata aacaccgatc tggtcattgg cgaaaaaatt
       61 catttcagga cactaagcaa tcgttcttta tattttaacc tcacccaacc cccattaatc
      121 gccacaataa aagatgatgt gttttgcact cttcatgatt actataacca caataatgaa
      181 acgaaatcca ctattgacaa ttacatatca aaaataaagg ataatcataa taccccatgg
      241 ttattaaaca acgaaaatgt tcaggaataa aaaaaagaga caattcccga gataaaacaa
      301 aagagcataa acatatgaac cggaaattct atttcaaaaa tatcctgtgg ggaatgtgcc
      361 ccattataat atccatattg catctgatat atgacacacc aacagaagca tcgataaaag
      421 ttgacttatt tctgatactc agtggcctgg cccttccttg ttcaatatac ctgacaaaaa
      481 caatcacact aaaatacaca tccatatcat tctggaaaaa acctcttttc tggtcttatg
      541 gtatatatca ttttcatctt gtttatataa tgacatgtat agttttagca ataccttctg
      601 caattatcgt tttattcttt cacttttatc aaaacatcac cagaaactgg aagtgacaac
      661 cgcaataatg aacataaaaa ggcaaatcag aatggcagta aatcaataaa gtaccatttc
      721 atatgaatca tcagaaaaac ggtggacgcg acatccagca cgaggcaaaa cacagcaaca
      781 aaataagccg gatatcgcgc cctgtacttt tctaactcaa ctcccggcat tgtgcaagtt
      841 ttaatcgcag gtggattcag agccacgccc ccattgattc agcctgaaga cacgtatatc
      901 cttggagaaa gcaggaatct cattgctgtg aaattcctta cagaatcttc agccccattt
      961 tatggggctt tttttggtgc ccccacgctg ttaccacact ttcgggatgc gaataaaaaa
     1021 gcccacagtt tttacactgc ggacttttgt tttttctttg tgatttgtgt gcaagggtgc
     1081 gactttcgac cgaacctttg gcaaccggat agcggggact gttgtccccg ctgcggtttc
     1141 ttacggttta cactgcaaga acgccgcaat ttccgaccac atgaagctta ccctgaattc
     1201 acacggtgat gcgtgtaaca cccagacggt cagcaacatg gtcaggcata acatgcccac
     1261 aatgatcgtt ttttgcggca tagcacttgc tccttttaca gagaggcgct aaccttccac
     1321 ttgctgaagg tagatggtca gggcctcggg ttaacgttta agttgactcg gggcctttcc
     1381 acatccggcc ttcaggtaat ccttccagcc gtcagccgaa aggcacccgc gcgtaatgta
     1441 ctggttttca gagaggtccg caatcttccg gtgacgagat catctgcaca cgccccggtg
     1501 ggacgggcta acggtatatt caggtgcagg atctctctgt ctggtgcgtt tttaaattgt
     1561 caaacgcacc ctggcggcaa aatcagccac cttttttcgc atcaacaaaa cgcccaaaac
     1621 gctgggtaaa gcgtgcaaca ttaccttctg aagcgacagt tctcagttta cctgtataaa
     1681 acggatgcga cttagaagaa acgtcaattg taacatatgg gtaagttaca ccatccagtt
     1741 caattacccg gtcagttttg atcgtcgaac cgatcctgaa gtactcatca atactggtat
     1801 cgtgaaatac tactgtacgg tattccggat ggatattcgg tttcacaaca gttttccccc
     1861 tttaagtgaa aattccgcat aaaggtacat attcagacgg tcagattcaa ctgtctgttc
     1921 ttataacctc actctctttc agtattacaa tatgttatat aattataaac tggacttccc
     1981 gaaaaaatcc attaaataag aagcgagccg ttgcggaggc ttgctctctc ctttaacctc
     2041 atgtgcaata tccgtaacgc gctcacataa atcatcatct gataatataa caacacccat
     2101 tattttattc tgacctaaaa acttcttatg aaataaacgt ttacccgacg tatctgatcc
     2161 cgctgttttt acctgtatcc tttaacatac ttgagaagcc cgctgaaaaa tataccgtaa
     2221 aggcggttct ctgtcttcca taaacctgtc ggataagaag ggcattaaat gaaaaccaaa
     2281 aaagttatga tgattgcact ggtcagctct gcgctggctc tttcaggatg tggagccatg
     2341 ggcacagcga tcaaaaaacg aaatctggaa gtaaaaaccc agatgagtga a
//
LOCUS       DAZGOB010000285         2376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGOB010000285 DAZGOB010000000
VERSION     DAZGOB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.287"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..681
                     /locus_tag="ACHLIF_005196"
     CDS             <1..681
                     /locus_tag="ACHLIF_005196"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9608999.1"
                     /translation="TPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNV
                     GRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRR
                     GLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKL
                     NVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAIMD
                     FMTARGYH"
     gene            complement(730..2229)
                     /gene="pitB"
                     /locus_tag="ACHLIF_005197"
     CDS             complement(730..2229)
                     /gene="pitB"
                     /locus_tag="ACHLIF_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO9609000.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            2166..>2376
                     /locus_tag="ACHLIF_005198"
     CDS             2166..>2376
                     /locus_tag="ACHLIF_005198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609001.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPL"
BASE COUNT          645 a          609 c          573 g          549 t
ORIGIN      
        1 acacctccat taattgccgt tattggtagc gatggttcag gcaagtcaac ggtgtgtgaa
       61 catcttatta ccgttgtcga aaaatatggt gctgccgaaa gagttcattt aggaaaacaa
      121 gccggaaatg tcggtcgtgc agtgacaaaa ttaccgttga tgggaaaatc cttacataaa
      181 acaattgaac gaaatcaggt gaaaacagca aaaaaattgc caggaccagt tccggcgctg
      241 gtaattacag cgtttgtcgc ccgtcgctta ctgcgctttc gtcatatgct tgcctgtcgt
      301 cgtcgcgggt taattgttct aaccgaccgt tatcctcagg accaaattcc tggcgcttac
      361 gatggtacgg tgttcccacc taacgttgaa ggtggtcgtt ttgtctcatg gctggcaagc
      421 caggaacgta aagcgtttca ctggatggcg agccataagc ctgatctggt catcaaactc
      481 aatgttgacc ttgaagttgc ctgcgcgcgt aagcctgacc acaaaaggga gtcgctggca
      541 agaaagatag ccataacgcc acagttaacc tttggtggtg cacaactggt tgatatcgat
      601 gccaatcagc cactggaaca ggtgttggtt gatgcagaaa aagcgattat ggattttatg
      661 accgcgcgtg gttatcacta gccaaaaatg gaaatgcccg atcgccagga ccgggcattt
      721 tcaggaaggt taaatcaact gcaatgctat ccagtacagc ccaccagaaa gaaaaatcgc
      781 cgccggtaaa gtaaataccc acgccatcag gatgctggtt accgttttac gctgtaaccc
      841 accgccgtcc accaccatcg tccctgcaac tgcagacgag aggacgtgtg ttgtggagac
      901 gggcatccca atataactgg caagaccgat agacactgcc gccgtcattt gtgccgccat
      961 gccttgcgca tacgtcatgc cgcgcttacc aatcttctca ccgatggtca tcgctacacg
     1021 acgccagcca atcatggtgc caatgccgag cgccagtgct accgccatga tgatccacac
     1081 cggagcgtac tcaatggtgc ttaacatatc gctgcgcagt tttttcagca ggttctggtc
     1141 ttctttactg acgcctggca gtttcgccag cttcgcggag gtatctgaga tgcacagcat
     1201 aatgcggcgc agctggctgc gctgactcac gcttaacggc tcgtaacttt ccatattgcc
     1261 tggcagcatc gttttaacgc gcgcaatcgc atcaaaggta tttgccggat gacagtgaaa
     1321 ctctgttact tgcgcgccat cagtcgatgc cgcaggcaat ggaggttcca tcgcaatcaa
     1381 cttctgcggc agttcaggat gctgttgcag atagtgttcg aagttggaga cggcatcgcg
     1441 agtacgggta atttcatagc cggacgcgtt catattgacg acgaagccag caggggcaat
     1501 ccccaccagt accagcatca ccaggccgat ccctttctgc ccgtcgttcg cgccgtgcga
     1561 aaacgccacg cccgcagcgg aaacaatcag cgcaatacgc gtccagaatg gcggtttacg
     1621 tttgcctttt ttctttttgc gatcttccgg aatgcggtga atacggtcac gctttttcgt
     1681 cccgctccag aaacggcgca gcaggaaaat cagaccaccc gcaatgacca ggccgacgat
     1741 aggggaaaca atcagcgagg agaaaatttt ggtcacttca cgcaggttta acgcatccat
     1801 caccgatgag ccggttaaca gcgcgttggt taaaccgatg ccgataatcg cgccaatcaa
     1861 ggtgtgcgaa ctggaggccg gtaaaccgaa gaaccacgtc cccaggttcc atataatcgc
     1921 cgccagcagc atggaaaaga ccatcgccag accgtgggtt gaccccatat tcagcaacaa
     1981 atcggttggt aacatatgga caatggcata ggcaacgcta agtccgccca ataacacgcc
     2041 aaaaaagtta aaaaatgccg ccatcaccac agcaagttgt ggttgcatgg cacgagtata
     2101 aataacggtt gccaccgcat tcgccgtgtc atgaaaacca ttgattgctt cgtagaacaa
     2161 cacaaatgcc agagcaagca ataacaaaag ccctgtgtat atatcaaggc caacaaataa
     2221 atttagcata tattagatta cgccattttg aatttacgaa cggacgcatt atcagtgact
     2281 ttaacggcat gggcaaagtg aaatatcatt ttttaacgat aaaaagagtg ttatttcttg
     2341 tgtatcagac aataagcatt cattcaaaac ctttag
//
LOCUS       DAZGOB010000286         2359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGOB010000286 DAZGOB010000000
VERSION     DAZGOB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.288"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..587)
                     /locus_tag="ACHLIF_005199"
     CDS             complement(<1..587)
                     /locus_tag="ACHLIF_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9609002.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVRVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLV"
     gene            complement(645..992)
                     /locus_tag="ACHLIF_005200"
     CDS             complement(645..992)
                     /locus_tag="ACHLIF_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO9609003.1"
                     /translation="MVTKTITEQRVEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(1029..>2359)
                     /locus_tag="ACHLIF_005201"
     CDS             complement(1029..>2359)
                     /locus_tag="ACHLIF_005201"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9609004.1"
                     /translation="EYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTGY
                     DGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTAC
                     SAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGNQ
                     FEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLAD
                     ELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLAT
                     MLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPEM
                     TVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAA
                     GSGKAEPVSASTTAMFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          466 a          754 c          602 g          537 t
ORIGIN      
        1 ttcacaagac cgctggcctg gtcgcagtag aggtcaagat catgtgtagg atcgaacgtg
       61 tcacgatcct gttttgacca ctccttacca ctaccctgag tgatgttatt cttcgtcgac
      121 ctgccaaaat cgacctcaat tttctcgaac tggtctcctt ccatcgtgta tttgccatac
      181 agcacggcat tcaccgcctg catttcttcc acccggacaa tggcgtgctc ttcctgtttg
      241 aggttatcag tgatgatacg cagacgacgg taggccgggt cgttcagctg agccggatct
      301 tcaccgggaa gacgctcaac cgcctgctgg taattaaatt cgtgtttcgg cttgacgtag
      361 cccggacgta acacgcgggt ttcaccacca cgatgacgca gcacttttcc ttcaacaacc
      421 ggggaaacat aggccgccac cggcgttttt ccggtaattt tgtccagcat cacctcttcg
      481 gtatggaaat tcaccgtacg gcggaaaaac agctccagaa acagcgcacg aaatttcact
      541 ttttgttcgg tataaccgag taactggcgg gtcgtaaaca atcccataaa tcagttcctt
      601 tcattcagaa atcagtcagg ccaccgcggt ggcctgataa cgtgttacgg cagcgccgcg
      661 tgactcaggg cactgccggc aaaggcgttg gcctttttgt gttcatccac actttcaggc
      721 cagcggattg cctccgtcgc aaaggtcccc gacttgtaat aggtcagcgc cgtctctgtg
      781 ccttcaagcg gcagtaccag tatgccaacc gcactaccgg ctttctgtcc gtcccagacc
      841 accagtttcc cggtggcctc atccagcatc aggggcgtca gtgccggtgt tgccgaggaa
      901 atcccgctgc tgcctgtggc ggtatgagcc ggatcattac cggcaaaaat acgtacttcc
      961 acacgctgtt cagtgatagt tttcgtcacc atattgtaaa aacctcctgt tgatggtcag
     1021 cactggcttc atggcatggc catgagcatt ttcacgtccg catcaccgtc tgctgacgtc
     1081 tgtggcacgc caccctgtac cgctgccggt gaatggttcg ccatgatgcg ttcaaacatg
     1141 gcggttgtgg atgcagagac cggttctgcc ttacctgatc ccgcagccag cacagcccgg
     1201 gcgctctcca cagtcattcc cgggcaggca gccagctgtt cagcctgcgc ctcagcccct
     1261 tttgcctcat ccagtgccat gatctgatca cggagtgagg gtccggcatc cgcctgcggt
     1321 gaagcagcca ggatcgggcg ggctttttcc accgtcatct ccggcatcgc cgccagcgtt
     1381 gccgccagtt gttcacgacc tttcgcttct tcacacgcca taatgcgatc ggcttcactc
     1441 tgcgcggatg ccaccggctg ctgtggtgcc gccgcggcca gaatcgcccg ggcctgttca
     1501 acgctcatgc cctgttgtcc tgccagcatc gtggcaagct gttcacgtcc tttcgcttcc
     1561 tggcatgtca ggatccccat cactcgctgg ttctcctgcg cggcggcttc cgttgcagtt
     1621 aattgcggca tagtgcctcc tctgacatta ctgttcagcg ccgtggccat cacactgatg
     1681 gcatccgacg cattgattaa ttcatccgcc agcccggcct caatgccgga ctgaccttca
     1741 aaaacggcgg cctctgttcc cgtgacagca tcaacagaca gaccggtata catcgctact
     1801 ttttcagcaa acatccggcg cgccgcatca atgcgctgtt gcatgtcctg gcgaacctct
     1861 gccggcaacg cttcaaactg attgccatcc accttgtgcg cccctgagta aatcagcgtg
     1921 atatcaacac cggcctgcgc cagatgaccg gcatagctga catggctcat catcacgcca
     1981 atggagccga tacgggatgt ctgggtaacc agccgtcggg agcaggccga cgccagcagc
     2041 atggctgcag agcaggccgt gtcattgcac agtgcccaga ccggcttctg ctgacggagg
     2101 cggtaaatca tgtcagcgca gtcaaacgcg ccggcggcct gcccgcccgg actgtcaatg
     2161 tccagcagta cgccccgcac ctggctatcc gccattgcct gctgaagaca ggcgacaatg
     2221 ccgtcatagc ctgtcattcc ggaaaatggc cgcatacccc ccagccggtg caccagcgtg
     2281 ccggtcaccg gcagtacagc aataccgttc accacccggt aaacacgggc cggtcgttta
     2341 cctccggcca tgtactcgt
//
LOCUS       DAZGOB010000287         2356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGOB010000287 DAZGOB010000000
VERSION     DAZGOB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.289"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(45..626)
                     /locus_tag="ACHLIF_005202"
     CDS             complement(45..626)
                     /locus_tag="ACHLIF_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9609005.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQA"
     gene            complement(623..1366)
                     /locus_tag="ACHLIF_005203"
     CDS             complement(623..1366)
                     /locus_tag="ACHLIF_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9609006.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVSRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMEGT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            complement(1377..2075)
                     /locus_tag="ACHLIF_005204"
     CDS             complement(1377..2075)
                     /locus_tag="ACHLIF_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9609007.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRSDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(2075..>2356)
                     /locus_tag="ACHLIF_005205"
     CDS             complement(2075..>2356)
                     /locus_tag="ACHLIF_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609008.1"
                     /translation="PSVVRVRFGDGYEQRRPTGLNPQLKTFQAVFRVTDESTRRWLEE
                     FLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYSDFSCTIEQVVN"
BASE COUNT          495 a          797 c          583 g          481 t
ORIGIN      
        1 ctcccgcagt tcctgtccgt gagcactgcg ggattttttc gcttttatgc ctgccgcccg
       61 ataaccacga cctttccgcc cccgccttca tcacgggtac tgatgtcctg ggatatacgg
      121 cgggagccaa ccagcatttc cccgtaaggc accggcatcg ggttaccctg ggcaatcatg
      181 ttgtccagcg acgaaaagta cgtgttctgt ttaccgttat ccgttgccct gtattccggt
      241 gtttttggct tcggggccag catctgtgcc acaccaccca gtatcatgct ggcacccagt
      301 gaaaacagca tcgtggtggc agaaaaaccg ccggcactca gcgctgcacc ccaggctgcc
      361 atcgatgctc cggccgtgaa gaaagagccc acgatggctg ccgcccccag cacaatctgc
      421 agtccaccct ttccggcccc ggccagtcgc ggcacaatgt ggatgaccgt tccctcaccc
      481 agctgttcgt gaagacgggc gtacaccgcc tccggtgccg tgtcatcacc ggcaatacgt
      541 atctggtacc agccttcgtt catctgacgg cggaatccgg gcacctgtaa cgacagcgcc
      601 cggatggctt ccgctgccgt gttcacatac aggctgaggc ggcggccaaa tcgttgtaaa
      661 tccccgtgaa ggcagatgcg tgccagtggc ggtgacgcca gacagaatgc gttcgtcgtt
      721 gccatttttc ggaatacctc tcccgtttac tcagttgttc aggcagatgg tgaagcagct
      781 caccgttgcc acagtaaatg gcggcatggt tcggtaccga agcaccaaag cagcacagca
      841 gaatatcgcc cgcctgtgca gaggacaggg gcacccggta aaagcccgtt ccctccatat
      901 tgtccaggta caggttctgg ccgttgcgcc accagtcatc ctcgcgatga aaatccggca
      961 tttcagtccc cgccagatgg tacgcatccc ggaacagggt gtaacagtcc gtcaccccgt
     1021 gttcaaagcg ccgtccggtc aggtgcggaa cgcagcggaa tttgtgaatg tcaccccggc
     1081 tgaccagcca ccagggcagt gcgcttttta tctgcagccg ccggtcagcc tcgctcagcc
     1141 agggcagacc accgggatga ctgtgaacca gcgccacaat ctccccctgc atctctgcct
     1201 gcagccagtc ttccggtgcg atacgaaaat acgcctccgg ctctgcggaa atattcacac
     1261 aagggatata ccactccccc tccggcgtgc ttatcacgaa gccgcacgac tccgcaggcg
     1321 cacaccgccg ggcatgcgcc agaatcgctg attcagtctg tgtcataaac cgggatttac
     1381 tgcgaaagtt tattaatgga aaggaagccg ccaaagttgc cgacattcct gcgcagttca
     1441 cacccgcgca tgcatttact gcatctgtcc ttccggatat ccgtggtggg gttgtcgaac
     1501 tcatccgcca ctgccggacc gttatacccg cattcatcag agcggtaatc ccacatacag
     1561 gtgttcgcca gcatgatgcg accgggaaac agcgcaccgt ccgtctccgt cggtgtcgcc
     1621 agcacaaacg aggccgtcat ggccgtcagc gatgacatct gctccaccac ccaccggtcc
     1681 gtcagctcct gctccgggtc tgcctcagga ttgcctgcca caaaattcac cgcatccaga
     1741 aaacgcgcat acacccggcg acggaccacc gtggcaccca ccaggctctg caaatcctcc
     1801 gccatcccgg tgacaaggcc gaaaagattg gacactgtca gcgacgggcg ggcactgctg
     1861 ccctttccgt tcatctcaaa gccactcccc tcaatcgggt acgcctgata ttcacgcccc
     1921 tgccaggtca ccggctcccc tttttcattc agctcattac agaaaaaata ccgctcacca
     1981 ccctgtaccg tcaggtcgat ttcccagagt accacccgcg gtgactgctc tgacttaacc
     2041 gactcgttca gactttcttc gcgaatatcc tgcatcagtt caccacctgc tcaatcgtac
     2101 aactgaaatc actgtacctg gcgttatctg tgacgctcca ctcccggcac accaccctca
     2161 ccgtccggtt atgtttcggc ggtcgccaca aaaaggcacg gtaaccacca tgccacgata
     2221 aaaactcttc cagccagcgc cgggttgact catccgtcac ccggaacacc gcctgaaacg
     2281 tcttcagttg aggattcagc cctgtggggc ggcgctgttc ataaccgtca ccaaaccgca
     2341 ccctcaccac cgacgg
//
LOCUS       DAZGOB010000288         2349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGOB010000288 DAZGOB010000000
VERSION     DAZGOB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.290"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(87..1499)
                     /gene="pykF"
                     /locus_tag="ACHLIF_005206"
     CDS             complement(87..1499)
                     /gene="pykF"
                     /locus_tag="ACHLIF_005206"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO9609009.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1507..1701)
                     /locus_tag="ACHLIF_005207"
     CDS             complement(1507..1701)
                     /locus_tag="ACHLIF_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609010.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2056..2265
                     /gene="fumD"
                     /locus_tag="ACHLIF_005208"
     CDS             2056..2265
                     /gene="fumD"
                     /locus_tag="ACHLIF_005208"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO9609011.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          617 a          511 c          570 g          651 t
ORIGIN      
        1 attttttatt gcttctggtt atcgattaaa taaaaaaagc gcccatcagg gcgcttcgat
       61 atacaaatta attcacaaaa gcaatattac aggacgtgaa cagatgcggt gttagtagtg
      121 ccgctcggta ccagtgcacc agaaaccata actacaacgt cacctttgtg tgccagaccg
      181 ctctgcagag ccagttcttt acccagacgg tagaaatcat cagtagaagt gatctcttta
      241 accagctgcg gcacaacgcc tttgctcagt accaactgat gagccgtttt ttcgttggtg
      301 gtcagtgcca ggatggtggc atccgggaag tatttacgta ctgcgcgagc agatttaccg
      361 ccctgagtag caaccacgat cagcggagca tccagttttt cagcagtttc aacggcacca
      421 cggcataccg cttcggtaat gcgcagttta cggttgtcat tgttgaactc gagacggctg
      481 ttcatcacgc ggtcggtacg ttcgcaaatg gtcgccatga tagaaaccgc ttccagcggg
      541 tatttacctt ttgcggattc accagacagc atcactgcat cagtaccgtc gaggatggcg
      601 tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt ttttgatcat ggaatccagc
      661 atctgggtcg cagtgataac gactttacgt gcacggatac atttttcgat catcatcttc
      721 tgggcgaaga taacttcttc taccgggatt tctacaccca ggtcgccacg cgcaaccatg
      781 atgccgtcag aggcttcgag gatttcgtcg aagttgttga ggccttcctg gttttcgatt
      841 ttggagatga tgtggatgtt ttcgccgccg tgcgctttca ggtgctcacg gatttcgata
      901 acgtcagaac gcttacgaat aaaggaagca gcaacaaagt ctacgccttg ttcgcaacca
      961 aagatcaggt cctgtttgtc tttttcagcc agtgctggca gagcaatgga aacgccaggc
     1021 aggttcacac ctttgttttc gcccaggtca ccgttgttca gcactttaca gataactttg
     1081 ttaccttcaa tggcggtaac ttccataccg atcagaccat cgtcaaccag tacggtgttg
     1141 ccaactgaca ggtcagtagt gaaaccttca tacgttaccg caaccatttc gctgttgccg
     1201 ataacagatt tatcagtggt gaaagtaaag gtctgaccag ctttcagaga aacgtcgtta
     1261 ccgccttcca gtttcatggt gcggatttcc ggacctttgg tatcaagcag gatagcggcg
     1321 gttttaccag ttttgctcat cacgttgcgc agattctgaa tgcgctgacc gtgttctgca
     1381 tagtcaccat gagagaagtt cagacgcata acgttcatgc cagcgtccag cattttagct
     1441 aacatctctt cagattcggt tttcggtccg atggtgcaaa caattttggt ctttttcatg
     1501 acagtcttag tctttaagtt gagaaggatg ggagaaactt gctttctggg cgcactgccg
     1561 gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct ctaaggatag gtgacatcga
     1621 aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa cgtgagtgcc tgtttttgag
     1681 tcgttgagtt ctacagtcca aagtgctgaa accattcaag agtcaattgg cgcgcattat
     1741 acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt caaaaaatat acaacttgtt
     1801 agacaataaa ttgttatcaa agatttaacg ttccagggaa aaggttacgc aaaaacttcg
     1861 ctgtttgcgc aacgtctggt gccaaagttg ttagtgtgag gataagtgaa agttcatccg
     1921 gtgtataaat atatcatcgc tcaattgcta tagttaaaat aatttatcgc gttatttgtc
     1981 tgattttgtt tgatttttgt ttaattggtt agattgatat atgctgatag ttggcatgat
     2041 aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg agaaatgtgc
     2101 agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc aaagcatatt
     2161 gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg cagtgagctg
     2221 gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt caggatgagg
     2281 cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaatca ttctaaataa
     2341 caactgctg
//
LOCUS       DAZGOB010000289         2346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGOB010000289 DAZGOB010000000
VERSION     DAZGOB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.291"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..110
                     /locus_tag="ACHLIF_005209"
     CDS             <1..110
                     /locus_tag="ACHLIF_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609012.1"
                     /translation="VEILKEFNICPEKVAEYLSEFSGKHFLDVYNYYSN"
     gene            292..477
                     /locus_tag="ACHLIF_005210"
     CDS             292..477
                     /locus_tag="ACHLIF_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9609013.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            718..1707
                     /gene="nleB"
                     /locus_tag="ACHLIF_005211"
     CDS             718..1707
                     /gene="nleB"
                     /locus_tag="ACHLIF_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO9609014.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFLSLENEYQNIKIIYRADVDFSMYDKKLSDIYLENIHKQESYPASERDNYLLGLLRE
                     ELKNIPEGKDSLIESYAEKREHTWFDFFRNLAMLKAGTLFTETGKTGCHNISPCSGCI
                     YLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKS
                     KVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTSSSW"
     gene            1756..>2346
                     /gene="nleE"
                     /locus_tag="ACHLIF_005212"
     CDS             1756..>2346
                     /gene="nleE"
                     /locus_tag="ACHLIF_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO9609015.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIARE"
BASE COUNT          817 a          372 c          449 g          708 t
ORIGIN      
        1 atgtagaaat tttaaaagaa ttcaatatat gcccagaaaa ggtagcagaa tacctaagcg
       61 agttcagcgg aaaacatttc ctcgatgtat ataattatta ttccaattga aaaaaatata
      121 aagtatctat cacggcactc tcagttgagt atcacaggca catgtatttt agccccctga
      181 gtctacagac aaccaatatt acttaaataa gtgataatct taatgctgtt ttttagaaca
      241 gatgattgat atttttgggc cggagaaacg cagacaggct ttgttgaata aatcgagctt
      301 ttgctgagtt taaggatcag atcactcatt ttcctgcaaa agctcattgg gcgaaacaat
      361 ctgactcaga gaacccggat taaacgtctg gcccgcagaa ccatctattt ctcacgcact
      421 gtagtacgta ataaaaaagt catcggtact tttatcaaaa aacatatatt ctattaatta
      481 ttgccatcac ctttttctta ttgacaacat tactctgctg attgttaaaa tctaacctgt
      541 attcagggtt acgattctat acaatagaac agggagggga aaggatgtca tcgttaatga
      601 gactttttgt taatgtcaac aatgctatga atttatttta gaaaaacaat taagctgacg
      661 tagtgttccg tctaaatgac ggggcgatat taacatgatt agaacaagag gaattttatg
      721 ttatcttcat taaatgtcct tcaatccagc ttcagaggaa agacagcctt atcaaatagt
      781 acacttctcc agaaagtttc ttttgctgga aaagaatatc ctctggaacc tattgatgaa
      841 aaaaccccta ttctttttca gtggtttgaa gcaaggccag agcgatacga aaaaggagaa
      901 gtaccaatat tgaataccaa agaacatccg tatttgagca atattataaa tgctgcaaaa
      961 atagaaaatg agcgtataat cggtgtgctg gtagatggaa attttactta tgaacaaaaa
     1021 aaggaatttc tcagtcttga aaatgaatat caaaatataa aaataatcta ccgagcagat
     1081 gtggatttca gcatgtatga taaaaaacta tctgatattt accttgaaaa tatccataaa
     1141 caagaatcat accctgccag tgagagggat aattatctgt taggcttatt aagagaagag
     1201 ttaaaaaata tcccagaagg taaggactct ttgattgagt catatgcaga aaaaagagaa
     1261 catacttggt ttgatttttt caggaatttg gccatgttga aggctggaac tttgtttaca
     1321 gagacgggaa aaactggatg ccataacata tcgccctgta gcggatgtat atatcttgat
     1381 gccgacatga ttattaccga taaattagga gtcctgtatg ctcctgatgg tatcgctgtg
     1441 catgtagatt gtaatgatga gataaaaagt cttgaaaatg gtgcgatagt tgtcaatcgt
     1501 agtaatcatc cagcattact tgcaggcctc gatattatga agagtaaagt tgacgctcat
     1561 ccatattatg atggtctagg aaagggtatc aagcggcatt ttaactattc atcgttacac
     1621 gattataatg ctttttgtga ttttattgaa tttaagcatg aaaatattat accgaatacc
     1681 agtatgtata ccagcagttc atggtaagtc ttccggttgt ggttgaaaaa tatcgttctt
     1741 taggtataga ttcagatgat taatcctgtt actaatactc agggcgtgtc ccctataaat
     1801 actaaatatg ctgaacatgt ggtgaaaaat atttacccgg aaattaaaca tgattacttt
     1861 aatgaatcac ccaatatata tgataagaag tatatatccg gtataaccag aggagtagct
     1921 gaactaaaac aggaagaatt tgttaacgag aaagccagac ggttttctta tatgaagact
     1981 atgtattctg tatgtccaga agcgtttgaa cctatttcca gaaatgaagc cagtacaccg
     2041 gaaggaagct ggctaacagt tatatccgga aaacgcccaa tggggcagtt ttctgtagat
     2101 agtttataca atcctgattt acatgcatta tgtgagcttc cggacatttg ttgtaagatc
     2161 ttccctaaag aaaataatga ttttttatac atagttgttg tgtacagaaa tgacagccct
     2221 ctaggagaac aacgggcaaa tagatttata gaattatata atataaaaag agatatcatg
     2281 caggaattaa attatgagtt accagagtta aaggcagtaa aatctgaaat gattatcgca
     2341 cgtgaa
//
LOCUS       DAZGOB010000290         2346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGOB010000290 DAZGOB010000000
VERSION     DAZGOB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.292"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            228..419
                     /locus_tag="ACHLIF_005213"
     CDS             228..419
                     /locus_tag="ACHLIF_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9609016.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(472..705)
                     /locus_tag="ACHLIF_005214"
     CDS             complement(472..705)
                     /locus_tag="ACHLIF_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609017.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(801..1424)
                     /locus_tag="ACHLIF_005215"
     CDS             complement(801..1424)
                     /locus_tag="ACHLIF_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9609018.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1513..2022)
                     /locus_tag="ACHLIF_005216"
     CDS             complement(1513..2022)
                     /locus_tag="ACHLIF_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609019.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          683 a          603 c          498 g          562 t
ORIGIN      
        1 tttttgattt cagtcattga aagttgcaat gtttgacgat tcaacagcat ttaagtgagt
       61 ttcaggatat gggaaggaca tgaaaggagg agcgcaataa aatacacagg aagaagggga
      121 aaatgtttcg taaaaacaga aaattaacag cgcacatcag gaaacgcagt ctcctgattc
      181 agtgctacca catgaaaatt tagttaactg ttaccgtact aaaatttatg tcagaagatg
      241 ttccattacc gaaagtcaat cagcgataca aagacgatca tggtgcgctt gtcaccctga
      301 ccagtgttga ggaaacccgg gtggtgttta tgcaggacgg ctatccgcat ccctgcatgc
      361 gaccgatgta caactttctg ggaaaattca aacccgaacc ccgggaagaa acagaataaa
      421 acgaaatccc tgccggcaat cacactacct gatagcagca taatccactt attaattctc
      481 atccccggag acaagcccag aaaaccactc cgggtgttca ctgtatgccg tgttcagcgc
      541 atccctggaa acgtttatat aaaagctgtc ctgcaatttg cgggtaacaa cttctgccag
      601 gcgattccgg ttatccacca gtccggcata acctctctct gccagccact cattcagttc
      661 atgttccaga gacagattga taatatcata atcggacctt gccattcccg tttccttagc
      721 acatgttttc aggcacgggg ataaagtata aagaatgtca tactgctgct gccagagcaa
      781 tattccccct gatggttgta tcagagatgg tatgccggta ctttgcgggt tgttcccgtc
      841 aggttatcgt gcacctccac ggtcgtggtc accaccggca ttccggcctt tctcagcctc
      901 agaacatcag ccgcaatacg ctgactgcca aaccagaaca ggccgtccag tgcagtcacc
      961 agtaaacccg cctccagtgc atacttcagc cgttcacggg gcgctttcac ttcccgggca
     1021 acctgctgat atggcaatga tgtgttttca ttcccaacca cgcgacgaat acggtgagac
     1081 agatgatacc ggtatgtatc cggcacacca gaaaggctgg ccttcaggct gtacacgcag
     1141 ccaaaccgtt tatcgttaaa caccacattt ttctggctga tgccccattc ttcacgcagc
     1201 gcggcaatca attgcggtgt acgggtaagc agcaagcgaa aaggcagttc aaaactggtg
     1261 acgtaatcca cattcagcag tgcaatacgc aaccgttctt ctggcccggc ctctgtctgc
     1321 cggcactcct ccagaacatc ctgccactgc cggcgaagac ggggggattc gttccgttct
     1381 gtaaaacagt atttatccgc cagatagtca attcgtgtac gcatactgaa aggaattccg
     1441 tataaagatt cagcagacaa aactttatca gccagtaaaa attaacggaa gcattgatat
     1501 tcctcccgac aatcaccgga tgattgttgc aataacctgc gacataaagg actgaacagc
     1561 cgtttttaac gccacgtatt gccctgatga accaggccgg gccacccgaa aacatctttc
     1621 cacccggcat tgtccgccag cacttatcac cgaccaggtc tgttgcagta aatccaccag
     1681 ttgcgcacat tcttcatcag ccatgacgcc gactcgcgga tggcaaatac tcgtctggct
     1741 gagcagcaac agacatcgta tctgtgctcc ttcatgttgt tcccgcaacc agcgtaatac
     1801 caccggcgct gatgggctga ccgtgtgatt atgctccgta atacgttcgt gtacccgtcg
     1861 gtgaaaggaa ctacaaaatg tctgaatctg ttgaccatag attccttctg ccgaatagag
     1921 cgtgaagtga aatgccaaca gatttggcca gtggtccacc gcctgctgaa caaaacgccg
     1981 gatttccccc ggctctgaaa ttaaggcttc ggttatttgc acgaaatgtt ccttttcaac
     2041 ccctgttgaa ttttgtcaag ccggtgcaga cacagtaaag cgcgcatcaa cacctgaaag
     2101 gtacgataca aacacaagtt acaaacaata agaacggaag gacaattaat cagtgaggaa
     2161 taataactaa attaaccaaa agaatgttac tgacaatact accaatactt aaatagcaat
     2221 atattaatta atgtaagaac agatgcccgg taggacatat acacaataca tccacaaaca
     2281 cactcttttc attaaatcaa tcacctgatt aaaccatgaa agtgtgatgt aagtcaaaat
     2341 tactga
//
LOCUS       DAZGOB010000291         2259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGOB010000291 DAZGOB010000000
VERSION     DAZGOB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.293"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            774..929
                     /gene="hokD"
                     /locus_tag="ACHLIF_005217"
     CDS             774..929
                     /gene="hokD"
                     /locus_tag="ACHLIF_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO9609020.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1096..1374
                     /locus_tag="ACHLIF_005218"
     CDS             1096..1374
                     /locus_tag="ACHLIF_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609021.1"
                     /translation="MAHDTKLHNSDDSAVFASRHGRRYRTFKSDWFQHPPCTEEQAEW
                     LIQCDRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            1376..>2259
                     /locus_tag="ACHLIF_005219"
     CDS             1376..>2259
                     /locus_tag="ACHLIF_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9609022.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVHQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLRAEKIRPLYRESDIVPGEQTAISILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPEPPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQG"
BASE COUNT          578 a          578 c          570 g          533 t
ORIGIN      
        1 tcttgaagat gtattaccag aaaaaacacc acaaattaat tcagcaaaaa aaatcattga
       61 cgatgcaata aacgaaagag aaaaagagat agaaaagcgt ttctaagcat aacaatagac
      121 tagtctctgg gaggccgtac cagcggcctt tcttcttccc gtaatctagt aaaatcgtcg
      181 gtttttaatc aattaattaa ctcatctatt accaactcct agttggttgc taatgatgtc
      241 atagtgtcgc taacggcctg aattactcag tccgttcttc gctcaaagcg ggctagaagg
      301 ttagcttgcg tcggacttgg cgtatttaaa gaagtgctgg tggtgacagg ttattatgtt
      361 ccatttctac agaacaaaat cacagaaact atacccaata gttgtattga atcactgacg
      421 agacagcctc atatttatca ggactggtgt acgtccaata caggaggttg tggtgctggt
      481 tctcaaatgt gcgctggcta ttgctgctgt aatggcaatt tattgccttg ctgttgttct
      541 tatggatcgc ctttctgact gatttcatat tggcgaggta acgggagtta agtagaatgg
      601 ctgcgggtgc ttgaggctat ctgtctcagg catgaacacc aaaggcagat agagaaaagc
      661 cccagttaac attacgcgtc ctgcaagacg cttaacatta atctgaggct caatctatga
      721 acggcaaatc taggttagcc tcttacgtgc cgaaaggcaa ggagaagcag gctatgaagc
      781 agcaaaaggc gatgttaatc gccctgatcg tcatctgttt aaccgtcata gtgacggcac
      841 tggtaacgag gaaagacctc tgcgaggtac gaatccgaac cggccagacg gaggtcgctg
      901 tcttcacagc ttacgaacct gaggagtaag agaccaggcg ggggagaatc cctcgccacc
      961 tctgatgtgt caggcatcct caacgcaccc gcacttaacc cgcttcggcg ggttttgttt
     1021 tttcctagca ttctggttta caattcgcac gccagcctga acaactggca cctgctgcgc
     1081 cagcagagac aaccgatggc gcacgatacc aaattacaca attctgatga ttctgccgtc
     1141 tttgccagca ggcacgggcg gcgttaccgc actttcaaat ctgactggtt ccagcatccc
     1201 ccatgcactg aagaacaggc cgagtggcta attcagtgcg accgcagaca cggatacgag
     1261 attaagaaag ccctcagcct cgattatcgt cactggataa tctccgtcag gcttccttac
     1321 tccgagcgcc caccgcgtcc gtcccgcaca ttccagcaac gcatctggag gtaacgtgcg
     1381 ggtattactt cgacctgttc tggtaccgga actcgggctg gtgatcgtta agccgggccg
     1441 tgaatccatg ccggtattcc acaatacccg ggtactggtg gagccggaac cgaaaagcat
     1501 gcgtaatctg ccgtccgggg ttgttcctgc cgttcaccag ccgctagtgg aagacaaaac
     1561 attgctgccg tttttcagta acgcacgggt gattcgtgct gctggtggtg ctggtgcatt
     1621 gtctgactgg ctgttgcgcc atattaaatc ctgccagtgg ccacacggcg attatcatca
     1681 cagcgaaacc gtcattcacc gttatggtac cggcgcaatg gtgttgtgct ggcactgcga
     1741 caaccagctg cgtgaccaga catccgaatc actcgagcaa cttgctcatc aaaacctgtc
     1801 agcatggatg attgacgtca tcggtcacgc aataagcggt acgcaggagc gtgaattatc
     1861 tctggctgaa ttatcctggt gggcggtctg caatcaggtg gcggacgcgc ttccggaggc
     1921 agtattacgt cgttctctgg ggttacgtgc ggaaaaaatc cgccccttgt accgcgaaag
     1981 cgacatcgta ccgggagagc agaccgccat cagcatactg aaacagcgca caaaaaatct
     2041 tgcgccgctg cctcacgccc accagcaaca gaacccacca caggaaaaga cggtggtcag
     2101 cattgccgtt gatcctgagc ctccggaatc tttcatgaaa cgacctaaac gtcgccgctg
     2161 ggttaacgag aaatacacac gctgggtgaa gacacagccg tgtgcgtgtt gtggtaagcc
     2221 agccgacgat ccccatcacc tgattggtca tggtcaggg
//
LOCUS       DAZGOB010000292         2243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGOB010000292 DAZGOB010000000
VERSION     DAZGOB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.294"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>2243
                     /gene="agn43"
                     /locus_tag="ACHLIF_005220"
                     /gene_synonym="flu"
     CDS             <1..>2243
                     /gene="agn43"
                     /locus_tag="ACHLIF_005220"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO9609023.1"
                     /translation="TGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPG
                     ETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSG
                     GLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQV
                     VKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRTEGTANTTV
                     VYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQK
                     GRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLD
                     VLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSGAAVSGTRSDGKAFSIG
                     GGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSGKT
                     VNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDS
                     TVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAG
                     QIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTIL
                     NLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESW
                     YLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHD
                     NNGGIARG"
BASE COUNT          547 a          606 c          696 g          394 t
ORIGIN      
        1 acgggcgctt tcgtggttgc ctccgaactg gcccgcgcac ggggtaaacg tggcggtgtg
       61 gcggttgcac tgtctcttgc cgcagtcacg tcactcccgg tgctggctgc tgacatcgtt
      121 gtgcacccgg gagaaaccgt gaacggcgga acactggcaa atcatgacaa ccagattgtc
      181 ttcggtacga ccaacggaat gaccatcagt accgggctgg agtatgggcc ggataacgag
      241 gccaataccg gcgggcaatg ggtacaggat ggcggaacag ccaacaaaac gactgtcacc
      301 agtggtggtc ttcagagagt gaaccccggt ggaagtgtct cagacacggt tatcagtgcc
      361 ggaggcggac agagccttca gggacgggca gtgaacacca cgctgaatgg tggcgaacag
      421 tggatgcatg agggggcgat agccacagga accgtcatta atgataaggg ctggcaggtc
      481 gtcaagcccg gtacagtggc aacggatacc gttgttaata ccggggcgga agggggaccg
      541 gatgcagaaa acggtgatac cgggcagttt gttcgcgggg atgccgtacg cacaaccatc
      601 aataaaaacg gtcgccagat tgtgagaact gaaggaacgg caaataccac tgtggtttat
      661 gccggcggcg accagactgt acatggtcac gcactggata ccacgctgaa tgggggatac
      721 cagtatgtgc acaacggcgg tacagcgtct gacactgttg tgaacagtga cggctggcag
      781 attgtcaaaa acgggggtgt ggccgggaat accaccgtta atcagaaggg cagactgcag
      841 gtggacgccg gtggtacagc cacgaatgtc accctgaagc agggcggcgc actggttacc
      901 agtacggctg caaccgttac cggcataaac cgcctgggag cattctctgt tgtggagggt
      961 aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac cggacacaca
     1021 gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg tggcaccgcc
     1081 accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc cgctgtcagt
     1141 ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga tgccctgatg
     1201 ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga taccacggta
     1261 aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac gctgaataac
     1321 ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac catccgtgaa
     1381 ggcgatgcac tcctgcaggg aggctctctc accggtaacg gcagcgtgga aaaatcagga
     1441 agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa cctgaatgaa
     1501 ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca gcgcggtaca
     1561 gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac gaatgtcact
     1621 ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc ggtggtggat
     1681 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
     1741 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc
     1801 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggcggcag ggcaaccgga
     1861 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt
     1921 aagggtattc aggtggttga agccattaac ggtgccacca cggaggaagg ggcctttgtc
     1981 caggggaaca ggctgcaggc cggtgccttt aactactccc tcaaccggga cagtgatgag
     2041 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg
     2101 ctgacacagg caatggacta tgaccggatt gtggcaggct cccgcagcca tcagaccggt
     2161 gtaaatggtg aaaacaacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
     2221 aacaatggcg gtattgcccg tgg
//
LOCUS       DAZGOB010000293         2104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGOB010000293 DAZGOB010000000
VERSION     DAZGOB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.295"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..357)
                     /locus_tag="ACHLIF_005221"
     CDS             complement(<1..357)
                     /locus_tag="ACHLIF_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9609024.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVP"
     gene            485..1345
                     /locus_tag="ACHLIF_005222"
     CDS             485..1345
                     /locus_tag="ACHLIF_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9609025.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNMKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQIEDIISVINWAEKQACIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCIVSQLAFADGDALITGEMNELERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLNDDESKVFFEKVKAQHPEMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGTFFERVAA
                     VQTQWFKKYI"
BASE COUNT          561 a          437 c          525 g          581 t
ORIGIN      
        1 cggaaccaga cggacttcat cttttggcgg gcgtccgcgt cgcgcgcgca ctttttcgga
       61 caggatccgc acaaacggtc ttacaccatc cagatcaaat gattccggtg tcagcatccg
      121 ggcttcgcca atacgcattc cggtattcca gagggtggcg aacagcatat ggtgacgctg
      181 atccggcata tagaaaagaa gggcactcac ttccggggcc agcaggtagg ccggtgtggc
      241 ccctgtggaa gtggccattc ttctcaggga gagcgctgtt gcaaaatcca cccccggcgc
      301 aatgggtaac agggagacgc gttctggtga attctgcagg ggaatgacat tgttcatgag
      361 ttatttttca cgtagcacac gcactgttta tggatactga ttgcgctggg cattctggta
      421 ttgtgactgg tatgatgctg gcgctgattc tgatgattaa taaacaacat aacacaggat
      481 atttatgaaa attacggatc acaaactttc tgaaggtatt gctctgacat ttcgtgttcc
      541 tgaaggaaat atgaaacatc ctttgattat cttatgccat ggattttgcg gtatccggaa
      601 tgttctgctc ccttgctttg cgaatgcttt cacagaagca gggttcgcaa cgattacttt
      661 tgattatcgt ggattcggtg agagtgaagg agaacggggc cgtctggttc cagcaatgca
      721 gatcgaagat attatttcag ttatcaattg ggcagaaaag caggcatgta ttgataatca
      781 gcgtatcggg ttatggggaa catcgctggg tggttgtcat gtgttcagtg cagctgcgca
      841 agatcaaaga gtgaaatgta ttgtcagcca gctggctttt gctgatggtg atgctctgat
      901 taccggagag atgaatgaat tggagagagc ttcttttctg tccacactga ataaaatggc
      961 agagaaaaag aaaaacacag ggaaggagat gtttgtgggg gttacacggg tactaaatga
     1021 tgatgagtcg aaggtattct tcgagaaagt caaagctcag catcccgaaa tggatataaa
     1081 aatacctttc cttactgtca tggaaacgtt gcaatataaa ccggccgaga gcgcagccag
     1141 agtccagtgt ccagtattgg ttgttattgc tggccaggat agtgttaatc caccggaaca
     1201 gggaagagca ttatatgatg ctgttgcttc tggtactaaa gaattatatg aggaagctga
     1261 cgcatgccat tatgatattt atgagggcac attttttgag cgggtagcag ctgtgcaaac
     1321 acaatggttt aaaaaatata tttaatagtg agcctgtgtt taccattctg tgtaaatact
     1381 ttttcttaaa agtgaccgtc aaggcggtca ctaaacttta taataaagag gcatcgttca
     1441 tttccgggag gcagactgga ttgccagccc cccacttttt accaagctgc aggtatgggg
     1501 gacacggaca ctggtgttca cagaaaaaaa taaccatctc tgaaatgtct gaaccgcccc
     1561 ggttttcctg gagagtgttt tatctgtgaa ctcaggctgc cagatcatcg tttctgatgg
     1621 aagcataata agctttttct gcttctgccg gagggatatg acccagcctt caggaaaacc
     1681 ggggcggttc aaaccacttt tgcaccgatc gtgacatttt cccttaataa agcatctgta
     1741 actgaaacaa tcagcatatt aataatatat tgacaggcat cattgctgtc tgtgaaaaat
     1801 aagtatctac aaacatgcaa agccttttag ccagtgtctt cttcaggtca gtcgctggcc
     1861 cttttttatc atgatgtagg tgtatttatc tccagcacca gactttctat ctcaacgcca
     1921 tacgctgcat tttttgtaac atccgtcagc atcagcacta tgctgacgca tgacgtggag
     1981 cctgtgattg acacgctgaa gtcagtcaga aggttgttca gtaatcaggt gaccgcctcc
     2041 tgcctgcgcc tgtctgctgg agtcatagaa gaattacctg ttaacgacgg cgatatcatg
     2101 acat
//
LOCUS       DAZGOB010000294         2079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGOB010000294 DAZGOB010000000
VERSION     DAZGOB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.296"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(12..1564)
                     /locus_tag="ACHLIF_005223"
                     /pseudo
     CDS             complement(12..1564)
                     /locus_tag="ACHLIF_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1584..1931)
                     /gene="tnpB"
                     /locus_tag="ACHLIF_005224"
     CDS             complement(1584..1931)
                     /gene="tnpB"
                     /locus_tag="ACHLIF_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9609026.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(1928..>2079)
                     /locus_tag="ACHLIF_005225"
     CDS             complement(1928..>2079)
                     /locus_tag="ACHLIF_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9609027.1"
                     /translation="NKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIREM
                     KGGTR"
BASE COUNT          393 a          598 c          570 g          518 t
ORIGIN      
        1 tggggtaaaa attagtttac agattgagtg acattccagg gcaacaactc tttcacgcgg
       61 ttggcaggcc aggtgttgat tacactgatc acgtggcgta gccacgtttc cggctcgatt
      121 ccgttaagtt tgcagctacc gatcaggctg tacatcactg ccgcactctc gcctccacca
      181 tcagagccga agaacatgta gttacgccgc cccagtgcaa ctacccggag ggcgttttca
      241 cacaggttat tgtcgatctc cacccagcca ttgcggcagt attcgttcag agcgtcccat
      301 tgcttcagca gataggtgaa cgctttcgct gtatccgagt ggcgcgacag cgtgctcatc
      361 tgcccctgga gccactcata caacgactgc attagcggta ccgttctggc ttttctgacc
      421 gccagtcgct cttctgccgg actgccgcgg atctcagcct cgatagcgta cagttccccg
      481 atacgacgga gcgcttctcc cgttactgtc gttggatggc ggacatgtac atcgtggatc
      541 ttgcgtcggg catgtgccat acaagccgct tcggttacct gaccgctttc gtaaagagca
      601 ttgtaacccg catatgcatc ggcctgcagg atacctctgt agtccgccag atgttgctgt
      661 gggtggatgc ctttgcggtc gggagagtat gagaaccaca ccgctgccgg catggtcgaa
      721 ccggcgttgc gatcgtcacg aacatagacc cacagccgtc cggtacgggt tttaccctga
      781 cccggctcca gtacatttac cggcgtgtca tcggcatgca ctttacccgc catcagcaca
      841 tagtgcttca gttcatcata cagcgggcga agctgctctc ccatgatgtc aacccagcgc
      901 cccatcgtat tgcggtgcag ctccacgccc tggcgggcat agatttccga ctgacggtac
      961 agcggcagat gctcggcgaa cttagccatg attatgcggg ccagcagagc cggactggcg
     1021 taactgcgct cgatgggttt tggtggctgc ggagcctgaa ctatacagtc gcaccggctg
     1081 caggccagtt ttgggcgaac cgtttcgatt accctgaacg cggtgttgat gatatccagt
     1141 tgttcagaga tgctttctcc cagcggtttc agtttgccgc tgcagacggg gcattcggtt
     1201 tctgccgggg agataacctg cctgtcacgg ggaagtgttg ccggaagtgc tttgcggacg
     1261 ggagagtctg atgttttcgg cgctgtctct ccggccattg aggtgagttg caactgcgcc
     1321 tcaccaagcc tgttctggag ctcggttata cgcgtttctg cccgtgcgat cttcttttct
     1381 atcttctcgc ggcttttctc gctgctgcga ccgaacaaca ttctctgcag tttagcgacc
     1441 agcgctctga gtgagctgat ctcgcgggca tagccggtta tttcaccaga cagacgataa
     1501 cagcctgctg tgcgatcagc agggccttca gttgctcgat gtcgtcgggg agtgtgttgt
     1561 tcattcccct gttttatcac gggttatatc cggatgccag gccgttctgt ccgtttggga
     1621 tgttgccacg cgatcccctc cagtagcatg gataactgag ctggcgtcag gtgcactttc
     1681 ccttcccggg ttaccggcca gacgaagcgg ccccgttcca ggcgtttggc gaacaggcat
     1741 aacccgtcac gatcggccca cagtattttc accattttgc cactgcggcc ccggaagacg
     1801 aagatatgcc cggagaacgg gtcatctttc agcgtgttct gcaccttcga agccaggccg
     1861 ttgaagccac aacgcatatc tgtgatgcca gcgatgatcc agattctggt accggttggc
     1921 agcgttatca tcgggtacct cctttcattt cgcggattag cgcccgtaac atttccggag
     1981 tgagagggtc aaacagtttt accacacctg atttaagatg cagctcgcac cgtgggacgt
     2041 ttccgggatc actctcaggg cactcatcag gcttgttac
//
LOCUS       DAZGOB010000295         2058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGOB010000295 DAZGOB010000000
VERSION     DAZGOB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.297"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..184)
                     /locus_tag="ACHLIF_005226"
     CDS             complement(<1..184)
                     /locus_tag="ACHLIF_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9609028.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDII"
     gene            complement(184..399)
                     /locus_tag="ACHLIF_005227"
     CDS             complement(184..399)
                     /locus_tag="ACHLIF_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9609029.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            542..940
                     /locus_tag="ACHLIF_005228"
     CDS             542..940
                     /locus_tag="ACHLIF_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609030.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(1021..1179)
                     /locus_tag="ACHLIF_005229"
     CDS             complement(1021..1179)
                     /locus_tag="ACHLIF_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO9609031.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(1265..2008)
                     /locus_tag="ACHLIF_005230"
     CDS             complement(1265..2008)
                     /locus_tag="ACHLIF_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609032.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
BASE COUNT          690 a          513 c          342 g          513 t
ORIGIN      
        1 gaatgatgtc ttttccggtg tgtccgtgac atacagtcca tacgccaacg atatcttcgt
       61 atggtatgta gctgacacct tccaggccat cgtcaccact cggaccagtg atgagcacag
      121 acgctatggc aacagcccca ccaccaatag cagcagcaac agccttgcgt aatgatggcg
      181 acattattca cctctcgcag ccttacgctt atcttcttta atcttgaaat aaaggtttgt
      241 cagatacgtc agcaagccaa acaccaggct acccaatacg cctattgcca cccactggga
      301 tggggagact ttgtccagca actgcagtag ccagtatccc gtccccaccg ctgacgtggt
      361 gtatgacaca cctgttgtta ttttttccat ctggtacata ccccgtctcc cgttatccgg
      421 aagctgacaa caataaaaag ccaccagtta attcctgatg gccctgatgc ataaacgtca
      481 taatacctga ctgttatgat tgacaataat gataatgttt atatagaaag gttcccgatg
      541 tgtgttacat atcatttctc cacggggaat atccccacgc cagcgcagac tcttttaccc
      601 gttctcttct gcgctggctc ttttttatta tgctgctgca tttacctctg gcaccatgct
      661 ttctatctca acacaatacg tggtacttct tgtaaccaat atcataacga ttaatcgaca
      721 tagaatttct cccgtgtaca ggaacagagt taaaaagccg gaaccggaat caaatcacag
      781 gatgaccatc tgccagtggc aggtcataaa aaaaaggccg cgccatgcgc agccagaact
      841 cacaaggaaa atgatagaag gaaataacat tagtgatgta cgcatggcgc ctcccgctaa
      901 gttctgcaat gatcaaacag aactcgctac gtgcccttaa aactcgatca tttagcccct
      961 ccaaggagga ttcaccatgc ggttgatttt ttaataaaca gtaaacaaaa aagtcaagaa
     1021 ttattcattc tgttctttca tcatcggcca cagcaatacc acaatgccgc agaccagagc
     1081 gccatcagtc agtaccaaca ttatcctgct ggtgaaatcc atcatcacca tcactaaaag
     1141 caggatcaca acagcaagca gacacagttt ataaaacaat gttcagaaaa cgcattcagc
     1201 atgcctaagg ttctattcct acgaatagcc aacttgcaac ttaaaatatt atttatgcag
     1261 ccaattaaat tctggtcctt acaatatcaa cctgaagatt cttatcttgt gctgattgat
     1321 aaatgacaaa ccttttacta cctgcattga aagaagtaga caaaaccaga caattatcat
     1381 aacgagcaag aacataatac caaccatcat tataattaat catttcatat tctttcttaa
     1441 actgtggttt gtaatatcct gtcagaaatg aaaaaagcca aaaatatgcc acaaaagcaa
     1501 tcatcacaat ctcaaaaaaa tgttttttta taaatggctt atcatagaag catgataccg
     1561 ataaaaatcg cccataagat cttatcgaaa ttgtaaccgc cagcgcaatc gctgctgaca
     1621 gtagcaaaag aggtacctga atcttctgtc tcaatataga aaactcaata attgccggca
     1681 caaacaataa ttccacagca aaataaaggc gaaatacatt tagctcttgc atagaatgtt
     1741 ttcttttcac tgcgaaaaag aatacaacac caatacccca accgataaga aatatagcaa
     1801 tgacgataac tgcaaaaaat aaacttctgg caacatcatc aacacctgca cctacaatcc
     1861 accatgggaa gccgtagtaa aaagaagtac cccatccata gaaataagca ctcccccatc
     1921 caaggcatcc catgtaggca ataaaaagtg aagaactcct gagcagcgca ccatccttca
     1981 taaccacccc aatacaagat gataacattg gcttacaact cataacaaaa gcaattcaat
     2041 gccgtcaaga ggttacag
//
LOCUS       DAZGOB010000296         2040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGOB010000296 DAZGOB010000000
VERSION     DAZGOB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.298"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..1711
                     /locus_tag="ACHLIF_005231"
     CDS             2..1711
                     /locus_tag="ACHLIF_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO9609033.1"
                     /translation="MSGCGPESENKESQQQQPSTPTDQQVLAAQQAAIKEAEQRSVAD
                     KATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKAASQIANPGTARYKQF
                     DDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAVRVEEIVNYFPYDWDI
                     KDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKSEELPASNLVFLIDTS
                     GSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAEINAAID
                     SLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIESMVKKQ
                     RESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQTLITVA
                     KDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLFELTLNG
                     QKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTINAPSEDM
                     RFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFIRLIELADGVTD
                     ISQ"
     gene            complement(1782..>2040)
                     /locus_tag="ACHLIF_005232"
     CDS             complement(1782..>2040)
                     /locus_tag="ACHLIF_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO9609034.1"
                     /translation="MAAQEVIEQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQH
                     RLVNRNCYLDKYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          583 a          447 c          500 g          510 t
ORIGIN      
        1 tttgtcagga tgcgggccgg aatctgagaa taaggaaagt cagcaacaac agcccagtac
       61 ccccacagac cagcaagtgc ttgccgcgca acaagctgca ataaaagagg ccgagcaaag
      121 atccgtcgcc gataaagcca ctgcagacgc gaaagcaaaa gccttagccc agcaagaagc
      181 gcaacaatat tcagacaaac aggctttgca ggggcgctta caggcggctc caaaatatca
      241 acatgcagcc agagaaaaag cagcgtccca aatcgcaaat ccaggaaccg ctcgctacaa
      301 gcagttcgat gataatccgg ttaagcaggt agcgcaaaac ccgttggcga cgtttagcct
      361 tgacgtcgac accggcagtt atgcgaatgt acgccgtttt ctcaatcatg ggctgttacc
      421 tccgccagac gctgtgcggg tggaggagat agtcaattat ttcccgtatg actgggatat
      481 caaagacaaa caatctattc cggccactaa gccaatacct ttcgctatgc gctacgaact
      541 ggctcctgcg ccgtggaatg aacagctaac attgctgaaa attgatatcc tggcgaaaga
      601 tcataaaagt gaagagttac cagcttctaa tctggtcttt cttatcgaca cttctggttc
      661 aatgatttct gatgaacgtt tgccacttat ccagtcttcg ttgaaattat tggtcaaaga
      721 acttcgtgag caggataaca ttgccatcgt gacctacgct ggcgactccc gtattgcgtt
      781 gccttctatc tccggtagtc acaaggcgga aattaatgcc gcaattgatt cgctggatgc
      841 cgaaggcagt accaatggcg gtgccgggct ggaactggct tatcagcagg cggcgaaggg
      901 gtttattaag ggcggcatca atcgcatttt attagccact gacggtgact ttaacgttgg
      961 cattgacgat ccaaaatcga ttgaatcaat ggtcaaaaaa cagcgggagt ctggtgttac
     1021 tctgtcgacg tttggcgtgg ggaatagcaa ttacaacgag gcaatgatgg tgcgaattgc
     1081 cgatgttggt aacggcaact acagctacat tgataccctc tctgaagcgc agaaagtatt
     1141 gaatagtgaa atgcggcaga cgttgattac cgtagcaaaa gatgtcaaag cgcaaattga
     1201 gtttaacccc gcgtgggtaa cggaataccg tcagattggt tatgaaaagc gccaacttcg
     1261 ggcggaagat tttaataacg acaacgttga tgcgggggat attggcgcag gcaaacatat
     1321 aacgttgtta ttcgaattaa cgctgaacgg gcaaaaagca tcaattgata agttacgcta
     1381 tgccccggat aacaaatcag tgaaatcgga caaaacgaaa gaactggcct ggttaaagat
     1441 tcgctggaaa tacccgcagg gaaaagaaag tcagttagtt gaattcccgc tggggccaac
     1501 aataaacgcc ccctctgaag atatgcgttt ttgcgcagca gtagctgcat atgggcaaaa
     1561 gttacgcggt tctgaatacc tgaacaatac ctcctggcag cagatcaaac agtgggctca
     1621 gcaggcaaaa ggggaagatc cacagggtta cagggcggaa tttattcgcc tgattgaact
     1681 ggcggatggt gtgactgaca tcagtcagtg atgactgttt agcaaactat gttcgaccag
     1741 tcagcatatt tgctgactgg tcgaattgat taacaatgat gttaactcac tcttttgccg
     1801 gatgctctat ttttgggttg tgagtgtttt atttctaatt gtgtataaga agcattgatt
     1861 cgtgcatcgc catatttatc taaataacaa tttctgttta ccagtcgatg ttgaaaatca
     1921 atttcgtact gctcttctgc tgaatatttt ttaaatctgt cagcatagtt ttttaataac
     1981 gtataggggg cgctgtcaga attgctttcc ctttgttcaa tcacttcctg ggctgccatg
//
LOCUS       DAZGOB010000297         2027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGOB010000297 DAZGOB010000000
VERSION     DAZGOB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.299"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            552..824
                     /locus_tag="ACHLIF_005233"
     CDS             552..824
                     /locus_tag="ACHLIF_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609035.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLRCKKADECSGSICLPNVAIYAAGYRKSKQLTAR"
     gene            827..1189
                     /locus_tag="ACHLIF_005234"
     CDS             827..1189
                     /locus_tag="ACHLIF_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination N domain protein"
                     /protein_id="HFO9609036.1"
                     /translation="MGQEEKYELKKLIGEDAIEEIAALTTAIKNIRYALNTLISSCDK
                     NSREFLILGAALGIVDAATLHLITHDDILIEPYETLLLVRQKMADAAANGDLQLYIDL
                     RKVLRRMVKTEGDIPLTK"
     gene            complement(1220..1714)
                     /locus_tag="ACHLIF_005235"
     CDS             complement(1220..1714)
                     /locus_tag="ACHLIF_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609037.1"
                     /translation="MDVLSGITAAKQAYDLLKTIKETRDDAVIAKAIGDLHQRITDLQ
                     MLNAELSGLYQAEKEIAMKLRDENRKIKMFVVQAENYELHTTEGGSVVYRPKSHSDPS
                     IQQHNLCAHCFGEHKISILQPSTVTIKSNGFFVHSCPRCKNEYRMYRAPDPKPVYVPP
                     LTNY"
BASE COUNT          661 a          437 c          436 g          493 t
ORIGIN      
        1 atacacaaca gatctaattg aacagtataa aataccaacc ccaaacgaga gaaaagcaat
       61 agatcaatat cgtattgact tagaaatcga cgaaatgcta agcaaaagaa aagactaaaa
      121 acacccggcc tcagcgccgg gttttctttt cctgccgttc accacccaat caaccatcct
      181 catcatagac aagcatcaag ccaaaggtaa caccttcacc ccgcacgcaa gccctcaaac
      241 accaatcaat cagcaacatt tacaaaaata aaataccttt gttatccatc acttataacc
      301 tatttaccat aaaatataaa ttaggttatt gaccacacct ataacctaag ttatctttaa
      361 gccatcagca ggacgctgga agccaaacgg aacagattgg caggctcttt aacattgatg
      421 ggattgtccc gccgaaatgc gggaaccaaa gagtagttgg ctttggggtg acgtgaagtg
      481 cagctgcaag acggcaaccg gaagataagc acgcggcgcg tcaccgccaa agtcaatcat
      541 cggaggtaaa catgacagta gtcattacat atctggctga cgataacgcc agaaatcgcc
      601 gcagagcacg cagacaggct caacgtgaac aggcaatgca agaacagcga ttggcacgaa
      661 aaattgcgct aaagctctct ggttgcgtca gagcagataa agcagcatca ctcggaagcc
      721 ttcgctgcaa gaaggcagat gaatgcagtg gaagtatttg cctgccaaac gtagccattt
      781 acgcggcagg ctaccggaaa tcaaaacaac tgacggcgag gtaattatgg gtcaggaaga
      841 aaaatatgag cttaaaaagc tcattggaga agacgccata gaagaaattg cagcattaac
      901 aacagctata aagaatatta ggtatgcgct aaatacgctt atctcctcat gcgacaaaaa
      961 tagcagggaa tttttgatac ttggcgcagc tctaggaata gttgatgcgg caacgcttca
     1021 cctaattact catgacgata ttcttattga gccgtatgaa acattactgc ttgtcaggca
     1081 aaaaatggct gatgccgcag caaatggaga ccttcaactt tacatcgact taaggaaagt
     1141 attaaggcga atggtcaaaa ctgaaggaga tatccccctg acaaagtaag ggggtgaaga
     1201 ggccatttca ggaataaaat taataatttg tcagcggcgg tacatataca ggcttagggt
     1261 ctggcgctct atacatccgg tattcattct tacagcgagg acatgaatgc acaaagaacc
     1321 cattagattt tatagtaacc gtgcttggtt gaagtatcga tattttatgt tcgccgaagc
     1381 aatgtgcaca aaggttatgc tgctgaatag aaggatcgga atgactctta ggtcgataca
     1441 cgacagaacc accttcagtt gtatgaagtt cataattctc agcttgcaca acaaacatct
     1501 tgatttttct attttcatct cgaagcttca ttgcgatctc cttctcggcc tgataaaggc
     1561 cagagagctc cgcattaagc atctgtaagt cagttattct ttggtgtaga tcaccaatag
     1621 ctttagcgat aacagcatcg tctctggttt cttttattgt cttcagcaga tcataagcct
     1681 gtttcgcggc agttatacca gataacacat ccatataaat tactctctta ctgtaggggt
     1741 aagaggattt tactattttt ctcgctgtag gggtacacga gaaccaccga gcctgatgtg
     1801 gttaaaagac aggcacaatc tttactaccg caagccacgc agtgaaatgg gtgtgacttg
     1861 tgttggtcgc cagaaaatga aattagacag caaaccactt atttgaggtg agatatgaca
     1921 aaatcatgga gcgtaccttt tcctgaatca gaaactgaac atgatggaat gcctgttttc
     1981 tggagattcc aggcgacagt tgaagaagat gggataaaaa tattcgc
//
LOCUS       DAZGOB010000298         2017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGOB010000298 DAZGOB010000000
VERSION     DAZGOB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.300"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            164..916
                     /locus_tag="ACHLIF_005236"
     CDS             164..916
                     /locus_tag="ACHLIF_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFO9609038.1"
                     /translation="MTTVNQIPDANITPSLPPKIREAVDKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRAEIALGRETLDEFE
                     DLLAAQAAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            913..1467
                     /locus_tag="ACHLIF_005237"
     CDS             913..1467
                     /locus_tag="ACHLIF_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO9609039.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLDTEEK
                     PAAYASQLEDLRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEMRTPVISAAYKSIIDETGLTVDASMRADAS
                     ASFTPAQHKVFLARLNRLNEKGVC"
     gene            1469..1741
                     /locus_tag="ACHLIF_005238"
     CDS             1469..1741
                     /locus_tag="ACHLIF_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO9609040.1"
                     /translation="MALKCPECGAVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPRCNEQESDKAKIPEKQQQTLNRYGSASKLSNRQQIPV"
BASE COUNT          575 a          493 c          502 g          447 t
ORIGIN      
        1 tgaatagtat gtattaaagg aagcgtgtag atcagacatg acacctttct ctggctagcc
       61 agaacaaggt cattgttcag tcactgacac aatcctcctc ggtagtgcgc ttgtatggac
      121 cccggcacaa ttgacacaac aacaaaacac taccggagca gtcatgacta ctgttaacca
      181 gatccctgat gcaaacatta caccctccct cccaccgaaa attcgcgaag cggtggataa
      241 agttaaggca gcaaaagctg tctggcagga agaacgacga aaacaaactg aagctgctgc
      301 aatgattgaa actatccgta aacgtcagga agatacaaaa acggaaacgc aggcgcttaa
      361 tgatgaatgg cgaaacctgt ttcgtgagaa tcagggaaat atgacgccac gcatgaaaaa
      421 actgcgggca gaaatcgccc tcggacgcga aacactggat gagttcgaag atttgctggc
      481 agctcaggcg gcagaaaatg aattcctgcc ctggaaaact gcggatgctg caaaccacta
      541 catcagcgaa cataatcgcc tgattgaaac tcatgcagtg tggctctgga atgagtttat
      601 gaaggaacac ggtcagaaac ttattcagat ccttggactg ctgaaaatga ctctgggccg
      661 aagcgcttct gctgttatcg gtgtagttca taccgtaaac gaccccgaaa gtgtgctgaa
      721 gcaatttatc agcgagcaac tcaccactcc ggcactgtcc tgtaacgtat cttcaaccga
      781 tgatattacc ctgccgggga tcagcattta tgcggacgat aaagccatcc aggatgccag
      841 acaatccccc agccctgcag cgcgttcacg gatgcttaaa cagcgtgaca tggttaaagg
      901 gggcgagaag aaatgaatgc cggaaccatt actcaggaag cactcaacga ttaccgctcg
      961 gcgataaaaa gctggctgac actacgtaat acgcaaagta cctaccagct tcgtctggcg
     1021 gctctgctgg atactgaaga aaaaccggca gcatatgcca gccagcttga ggatcttcgt
     1081 gagcgtcttg cagtcctcga atggcagatt aactgtgctg cacgggatgg tctttatgct
     1141 catcagattg tgctggagag ttgtgttaca agcgcaacgg aaaacttcat gagcgagcat
     1201 ggtgatgcac tcactgacgc tctggctcct tttctttgcg ctccatacgg gcttgaggcg
     1261 gcaatgaaaa tattacgaac cgctgtagcc cgacaaacgg aaatgcgtac tccggtaatt
     1321 tcagcagcat ataagagcat tatcgacgaa accggattaa cggtggatgc atcaatgcgc
     1381 gctgatgctt cagccagttt caccccggca caacataaag ttttcctggc ccgccttaac
     1441 cgacttaatg aaaaaggagt gtgctgatat ggccctgaag tgtcctgaat gtggcgcggt
     1501 cgctcacgcc agaaccagcg cctatgaagc tccgtcggtt aaacgctcat ggtatcagtg
     1561 ccagaatctg gaatgttcct gcacatttac cgcactggaa agcgtggata cgataattat
     1621 gaagcctcgg tgcaatgaac aggaatcaga caaagcaaaa ataccggaaa aacaacagca
     1681 aactctcaat cgctatggct ccgcgtcaaa gctgtcaaac cgtcagcaaa ttcctgtctg
     1741 attagcaaaa tacgaccacg aagtcccggt ccagtaccgg gattttttac gcctttttcc
     1801 tggctggcct gagagcgtat gagtgcatat ctatggcgca tgaaaacgca tgagtctcat
     1861 gcgccatttt tgacgcgaaa gcccttgtgt ggtggcttct gagacgattt acggggtgca
     1921 tgaaaaccag tctgttaagc gaagcgggca ggcgggcggg gcattgcgcg caggaatgtg
     1981 tataccgttt tagtaatgat cgctgacatc tcacttt
//
LOCUS       DAZGOB010000299         1977 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGOB010000299 DAZGOB010000000
VERSION     DAZGOB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.301"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            233..370
                     /locus_tag="ACHLIF_005239"
                     /pseudo
     CDS             233..370
                     /locus_tag="ACHLIF_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(478..1713)
                     /gene="aslB"
                     /locus_tag="ACHLIF_005240"
     CDS             complement(478..1713)
                     /gene="aslB"
                     /locus_tag="ACHLIF_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO9609041.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(1860..1936)
                     /locus_tag="ACHLIF_005241"
     tRNA            complement(1860..1936)
                     /locus_tag="ACHLIF_005241"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1900..1902),aa:Pro,seq:tgg)
BASE COUNT          515 a          528 c          444 g          490 t
ORIGIN      
        1 actaaaaaaa gcatcaataa tcaacgcgat ataataaacc tgccttacat atcaactgcg
       61 ccagaggtag gattgaaaac gctctcctga ttttccaatt cattttctgg ataaataaat
      121 aatttatttt tgtcactatt atttatgtaa tcatcctgtc agggagaggg atctcaatta
      181 tcaatgctta attacgtcat cattttgatg acatgaaacc ggagcatttt gcatggaatt
      241 ttcgttttca cccaaacttc ttgttgttgc tgtcgccgcc gctcttcctc tcatggccag
      301 cgcagcagat accccgtcaa ctgccaccgc acgcaaaggc tttgccggat acgatcaccc
      361 aaaccagtat ccacagaacg ctgcaattta ttgaatttgc acgatcatgt aggccggata
      421 aggcgtttac gccgcatccg gcaatcaacc gcaggcggcc acaaaatggc cgctttttta
      481 cttactcacc accagcaaat gcgcatgcat aatgtcactg gccgggcgac cgtgcgccag
      541 caaatcagcc attgctttaa gatatggcgg tagatggcgg aaataacgct gatacccggc
      601 acacaaataa ttcagccccg gtttgccgct ggcatcgagc atgaagcggt gtttcgggca
      661 gcctccccag cacgctttta acacgttaca actgcgacac tgcgccggta attgcttaaa
      721 tttatcttca ccaaacacct gctgttgcgg ggaatcgatc atttctgcaa ttgtttgctg
      781 atgcatattc cccagccgat attgcggata aacatagtga tcgcaggcgt aaacgtcgcc
      841 gttgtgctca acaatcaccg agcgcccaca ggttggctga tgatggcaaa ccgcacccgg
      901 cgcaccgaca aaattggcaa acgcccattc gatattcatc acgaaaatct tgccgacgtc
      961 gcgtttgatc cagtggtcga atatcgccac cagaaattca ccaaattcat cggggcgcac
     1021 cgaccattcc gtcagttccc cctgaatatc accaggcgca tgtagtttca gtccatcgct
     1081 ggcagctgtt tcatcagcca ggcgctcgac caccggaata aactggatga attcgactcc
     1141 cgcatcgcac aaaaaatcat aaacctgcaa cggttgctgc gcgctggtgc ggttgacgca
     1201 gaccagcacg ttatagtcga catgatgttt ttgcagcagc gtcagggcac gcatcaccag
     1261 cttatgcgtt ggtctgccac ctttggtcac gcgatattga ttgtggatct cagccggacc
     1321 gtccagcgat aacccaacaa gaaaatgatt ttctgccaga aatgcacacc atttatcatc
     1381 gagcagcacg ccgttagtct ggaagctgtt acttatcttc ctgccagcac cgtatttcgc
     1441 ctgtaacttt acggcacggc ggtaaaaatc cagcccgagt aacgttggtt cgccgccctg
     1501 ccaggtaaaa gccacttcgt tttgcggttc gctggcagcg atatagtgac ggacatacgc
     1561 ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg cggtaaaggg attgtttttc
     1621 gagataaaaa cagtagtcac agttcagatt acaatcggaa ccactcggtt tcgccatcac
     1681 atgaaaagca cgcgttggaa cctgttgcag catggtcact ccttgcctga agtcatcctt
     1741 caatacgtta agggcgaaat cataccgtgg ggcaatgagt tattacgggc gtttattgcg
     1801 aagggagggt gacgaaatgc acagaaaaca aaaaaccccg ccgaagcggg gttcaaaatt
     1861 ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt gcgctaccaa
     1921 gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattctt taaagtc
//
LOCUS       DAZGOB010000300         1966 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGOB010000300 DAZGOB010000000
VERSION     DAZGOB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.302"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1230
                     /locus_tag="ACHLIF_005242"
     CDS             <1..1230
                     /locus_tag="ACHLIF_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9609042.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRL
                     TTIQNDRTRIQTIYQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGEDGGSVVFP
                     PVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDH
                     RGLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHR
                     YYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
     gene            1227..1796
                     /gene="ybfC"
                     /locus_tag="ACHLIF_005243"
     CDS             1227..1796
                     /gene="ybfC"
                     /locus_tag="ACHLIF_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO9609043.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
BASE COUNT          590 a          425 c          493 g          458 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtgtggcg acgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccgcaag tgacctggta cggctgggac
      121 ggcgaccgcc tgaccacaat acagaacgac agaacccgca tccagacgat ttatcagccg
      181 gggagcttca cgccactcat cagggttgaa accgccaccg gtgagcaggc gaaaacgcag
      241 cgccgcagcc tggcggatac ccttcagcag tccggcggcg aagacggtgg cagtgtggtg
      301 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      361 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatgcaa
      421 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      481 cgcggcctgc cgctggcgct tgtcagcacg gaaggggcaa cagaatggtg cgcagaatac
      541 gatgaatggg gcaacctgct gaatgaagag aacccgcatc agctgcagca gcttatccgc
      601 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
      661 gacccgctgc aggggaggta tatcactcag gatccgattg ggctgaaggg gggatggaat
      721 ttttatcagt atccgctgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
      781 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      841 ccaagtaaat tgccggacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      901 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      961 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgattcccag
     1021 tatccaggga tggatacaaa gacagcaagt gattacttgc cacagacaaa ttggccaaca
     1081 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
     1141 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taagatgaaa
     1201 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
     1261 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
     1321 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
     1381 ggagatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
     1441 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
     1501 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
     1561 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
     1621 agagcagtga tccagttcag ttgttggcag tccttgataa ttcaatcatg gtaaaatatt
     1681 ttaaagatga gaaaccaact tatgctcgaa tgacagcaca tctcccgaac aaaaatgagt
     1741 cttacgaatg tttaataaag atacagcatg aactaattcg tagtgaagag aaataaaatc
     1801 tcttcctgag aaaacaaatt aatcattgtg aaaaattata taactcatgg aataggtgtt
     1861 tattttgcgt tccatttgca gggaaagatc acgtaacgct acttttttgt actaaataat
     1921 tcgaatttta agttcaacaa ttgagatact acttattgtc taaagc
//
LOCUS       DAZGOB010000301         1959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGOB010000301 DAZGOB010000000
VERSION     DAZGOB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.303"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..771
                     /locus_tag="ACHLIF_005244"
     CDS             <1..771
                     /locus_tag="ACHLIF_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO9609044.1"
                     /translation="RTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGG
                     YLNLIHNASGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLETGLPFSITDNLML
                     EPQLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLR
                     DSAKHSVSELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGL
                     EARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            892..>1959
                     /locus_tag="ACHLIF_005245"
     CDS             892..>1959
                     /locus_tag="ACHLIF_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YeeR"
                     /protein_id="HFO9609045.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYC"
BASE COUNT          436 a          518 c          542 g          463 t
ORIGIN      
        1 agaacagagg ttgccggtat gtctgtgacc gcgggggtat atggtgctgc tggccattct
       61 tccgttgatg ttaaggatga tgacggctcc cgtgccggca cggtccggga tgatgccggc
      121 agcctgggcg gatacctgaa tctgatacac aacgcctccg gactgtgggc tgacattgtg
      181 gccctgggaa cccgccacag catgaaagcg tcaacggaca ataacgactt ccgcgcccgg
      241 ggttggggct ggctgggttc actggaaacc ggtctgccct tcagtatcac tgacaacctg
      301 atgctggagc cacaactgca gtatacctgg cagggacttt ccctggatga cggtaaggac
      361 aacgccggtt atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc cggtttccgt
      421 ctgggcagcc acaacgatat gacctttggc gaaggcacct catcccgtgc ccccctgcgt
      481 gacagtgcaa aacacagtgt gagtgaatta ccggtgaact ggtgggtaca gccttctgtt
      541 atccgcacct tcagctcccg gggagatatg cgtgtgggga cttccactgc aggcagcggg
      601 atgacgttct ctccctcaca gaatggcaca tcactggacc tgcaggccgg actggaagcc
      661 cgtgtccggg aaaatatcac cctgggcgtt caggccggtt atgcccacag cgtcagcggc
      721 agcagcgctg aagggtataa cggtcaggcc acactgaatg tgaccttctg acagaaccat
      781 cgcctctctg tggtcccggt catcatgacc gggacccgga ccggcgcaac ggatcttcaa
      841 cgccacattc gctggcatta acaataacat gatattcatc acggagtgac tatgttacag
      901 atagtcggcg cgctgatcct gctgatcgca ggatttgcca ttcttcgcct tttgttcaga
      961 gcattaatca gcacggcttc tgcgctggca gggctcatat tgctgtgtct gttcggcccg
     1021 gccttactgg ctggctatat caccgaacgc ataacccggt tgttccatat tcgctggctg
     1081 gcaggcgtat ttctgacgat tgccggaatg atcatcagct tcatgtgggg acttgatggt
     1141 aaacatatcg cgctggaggc tcacaccttt gactctgtga aatttattct gaccaccgct
     1201 ctcgccggtg gtctgctggc tgttcccctg cagatcaaaa acattcagca gaacgggatc
     1261 acaccagaag atatcagcaa ggaaattaac gggtattact gctgttttta tactgccttt
     1321 ttccttatgg cgtgttctgc atgcgcacca ttgatcgcgt tacagtacga tatttcaccg
     1381 tcactgatgt ggtggggcgg gttgttgtac tggctggctg cattagtgac gctgctatgg
     1441 gcggccagcc agatccaggc gctgaaaaaa ctgacctgtg ccatcagcca gacactggaa
     1501 gaacaaccgg tgctcaacag taaatcgtgg ctgaccagtt tgcaaaacga ttacagcctt
     1561 cctgactcac tgacggagcg catctggctg acgctcattt ctcaacggat ttcccgggga
     1621 gagctgaggg aatttgaact ggcagacgga aactggttac tgaacaatgc ctggtatgaa
     1681 agaaacatgg cagggtttaa cgaacagttg aaagagaacc tgtcattcac acctgatgaa
     1741 ctgaaaacgc tcttccggaa ccgcctgaat ttatcaccgg aagcgaatga cgattttctc
     1801 gatcgttgcc tggacggcgg tgactggtat cccttttcag aaggtcgccg ttttgtatca
     1861 ttccatcacg tggatgagct tcgtatctgt gcctcctgcg ggctgacaga agtacatcat
     1921 gccccggaaa atcataagcc ggatccggaa tggtactgc
//
LOCUS       DAZGOB010000302         1916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGOB010000302 DAZGOB010000000
VERSION     DAZGOB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.304"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            234..1850
                     /gene="tir"
                     /locus_tag="ACHLIF_005246"
     CDS             234..1850
                     /gene="tir"
                     /locus_tag="ACHLIF_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO9609046.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMG
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPITTDPDAAANTAEAAAKDQLT
                     KEAFQNPDNQKVNIDENGNAIPSGELKDDVVAQIAEQAKAAGEQARQEAIESNSQAQQ
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRN
                     DSLARISEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     RLGMGGLTGGGESAVSTANAAPTPGPARFV"
BASE COUNT          532 a          404 c          481 g          499 t
ORIGIN      
        1 ttattaaagt ttcacgtggc atttattttt atatcgcatg attattttca tttaattaca
       61 ctgatgtgta tttttctgca ttattttctt gcatcaaaaa tcagactgtg atttatttgg
      121 tcgatgttat gttcagccgt atatcgacta cgtactgtat accattacat tattatgtaa
      181 tagcaatata aaggattatt atctatttac ataaaataaa aaggagatat tttatgccta
      241 ttggtaatct tggccacaat cccaatgtga gagctttaat tccacctgca ccgccattac
      301 cttcacaaac cgacggtgca ggaggtgccc gtaatcagct cattaactca aatggcccga
      361 tggggtctcg tttgctattt acgcctataa ggaattctgt tgctgatgct gctgattctc
      421 gtgccagtga tattcccgga cttcctacaa atccactgcg ctttgctgcg tccgaggtat
      481 ctttgcatgg tgcgcttgaa gttcttcatg ataaaggggg gcttgatact cttaactctg
      541 ctattggatc ttcgttattc cgtgttgaaa ctcgggatga tggcagccat gttgctatcg
      601 ggcaaaaaaa tggcctcgag accactgttg ttttaagtga gcaagagttt tctagcttac
      661 agtcccttga tcctgaaggt aaaaacaaat ttgtatttac tggaggccgc ggtggcgcag
      721 ggcatgctat ggtcacggtt gcttcagata tcgccgaagc ccgtcagagg ataatagata
      781 aattagaacc aaaggataca aaggagacga aggagccagg ggatccaaat agtggcgagg
      841 gaaaaatcat tgaaattcat acctcaacct caacttctag cctccgtgca gatcctaaac
      901 tttggttgtc attggggact attgctgcag gtctgatagg gatggctgcg acggggattg
      961 cacaggctgt tgcgttgact ccagagccgg atgacccaat cactaccgac cctgatgctg
     1021 cagcaaacac agctgaagca gcggcaaaag atcagttaac gaaagaagca ttccagaacc
     1081 cagataacca gaaagttaat atcgatgaga acggaaatgc aattccgtcc ggggaactaa
     1141 aagatgatgt tgttgcgcaa atagcagaac aagctaaagc ggcgggtgaa caggccagac
     1201 aggaagctat tgaaagtaat tctcaggcgc agcaaaaata tgatgaacag catgctaaac
     1261 gcgaacagga aatgtctctt tcatcggggg ttggctacgg tattagtggt gcgctgattc
     1321 ttggcggggg aattggtgcc ggtgttactg ctgctcttca tcggaaaaac caaccggcag
     1381 aacaaacaat cactacacgt acggtagtcg ataatcagcc tacgaataac gcatctgcgc
     1441 agggcaatac tgacacaagt gggccagaag agtccccggc gagcagacgt aattcgaatg
     1501 ccagcctcgc atcgaacggg tctgacacct ccagcacggg cacggtagag aatccgtatg
     1561 ctgacgttgg aatgcccaga aatgattcac tggctcgcat ttcagaggaa cctatttatg
     1621 atgaggtcgc tgcagatcct aattatagcg tcattcaaca tttttcaggg aacagcccag
     1681 ttaccggaag gttagtggga accccagggc aaggtatcca aagtacttat gcgcttctgg
     1741 caagcagcgg cggattgcgt ttaggtatgg gaggattaac gggggggggc gagagcgcag
     1801 taagtactgc caatgccgca ccaacgccgg gacccgcacg tttcgtttaa atatatccgt
     1861 gagtatttag ttgaggttgg gaggggggga gttttactaa tattaatgtt tcagag
//
LOCUS       DAZGOB010000303         1912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGOB010000303 DAZGOB010000000
VERSION     DAZGOB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.305"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..432
                     /locus_tag="ACHLIF_005247"
     CDS             <1..432
                     /locus_tag="ACHLIF_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspJ"
                     /protein_id="HFO9609047.1"
                     /translation="FVAVRIQSNQFTDLKNKTIQGHKDTVAKVIDWYNPQKNAFGIMM
                     GTPRRSADIAKEESRNALNFMIMEKDTFNEKILNSNANLQKKYGTTENSSWVSASVGS
                     LLDKGAKVYPDISCSLRLGEPFIITLPETVRLDVNIHPLKK"
     gene            complement(445..666)
                     /locus_tag="ACHLIF_005248"
     CDS             complement(445..666)
                     /locus_tag="ACHLIF_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609048.1"
                     /translation="MDIQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQ
                     GEFDFYRQIYPVTGGEHKVEALMWTHLPA"
     gene            complement(750..1130)
                     /locus_tag="ACHLIF_005249"
     CDS             complement(750..1130)
                     /locus_tag="ACHLIF_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609049.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQ"
     gene            complement(1331..1906)
                     /locus_tag="ACHLIF_005250"
     CDS             complement(1331..1906)
                     /locus_tag="ACHLIF_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO9609050.1"
                     /translation="MPLTSDIGSRSFNLGLEVFRARIAANGRGDITVGGETVSIVYDA
                     TNGRFSSSGGNDGLLSELLILGFNNGPRALSERMLSMLSDSGEAQSQESIQDKISQCK
                     FPVSSGNFQCPPESIQCPITLERPEEGVFVKNSDSSAVCTLFDVDALSRVVNDGSVHP
                     LTRAPITPSMIVKPEECKYDPARGSFIIKDS"
BASE COUNT          612 a          420 c          335 g          545 t
ORIGIN      
        1 ttcgttgctg taagaatcca aagtaatcag tttactgatt taaaaaacaa gacaattcag
       61 ggtcataaag acactgtcgc aaaagtcata gactggtata acccacaaaa aaacgcattt
      121 ggtatcatga tgggcacacc aagacgttct gctgatatag caaaggaaga atccagaaac
      181 gctctaaatt tcatgataat ggaaaaggat acatttaatg aaaagatact taacagcaat
      241 gctaatttac aaaaaaaata cggcacaaca gaaaatagta gttgggtatc ggcttctgtt
      301 ggttctcttc tggacaaagg agccaaagta tatccagata tatcatgctc tctgagactg
      361 ggagaaccat tcattattac acttccagaa acagtcaggc tggatgttaa tatccaccca
      421 ctcaaaaaat gatcctgaac gtttttatgc aggcaaatgc gtccacatca aggcttcaac
      481 cttgtgctct ccccctgtca caggataaat ttgcctgtaa aaatcaaact ctccctgctc
      541 attaccaata ccaatcagtt gaatgcagtt gccatctaca ataaattttt ccgctgacgt
      601 aaccaaaata aaggcttccg gatgttcatt aggaaaggtg ttagcaagag tacactgaat
      661 atccatctcc aacaaaatct cctgattatt agcgtaaact catcgacgaa atcgttacta
      721 tcccctgggc acatatgtac cattgaatac tactgttctc cagattctga taatccatgg
      781 cgaacaataa gaacactaca gccgctttgc tgattagtta gccataacgg tgaactttgc
      841 tggatagttt gagaaatatc atttcgccgg aggcttatac actgattact caagcttaca
      901 actgcaggtg catcaaagcg ctccggacta tcagtatcac gcatagctgt aaacagaata
      961 taatcactac cagccatatc atattccgtt tcttttagtc ttaacaagcc atcctctctt
     1021 gtaatattaa ctgtaattaa agatgtctca tcatgatggg catagcgctg tgtattaaga
     1081 attaaatgct cttccagaaa ttttacaggg ttactacgta tttcattcat tatacaccga
     1141 agctctgaac gatgcggcac cagaaagtgc ccggtatttt gtgaaataat actacttacc
     1201 ttgattgttt tctctccagc tcatgaaaaa taactcgcgt attatattgt tttaccaacc
     1261 acaggccccg aatgcctgtg gtgtttttat ataaattatc ggaagttaat attgtttcat
     1321 ttgaaacatc ttaactatct tttataataa agcttcccct tgcagggtca tatttacact
     1381 cttctggttt aacaatcatt gatggggtta ttggagctcg tgtcagaggg tgaactgaac
     1441 cgtcattaac cacgcgagaa agtgcatcaa catcaaataa agtgcatact gctgaactat
     1501 ctgaattttt gacaaacact ccttcttcgg gtctctctag tgtaattgga cactgaatag
     1561 actctggcgg gcactggaaa tttcctgaac taacaggaaa cttacattga gatattttgt
     1621 cctgaatact ctcttgcgat tgtgcttcac ctgagtccga aagcatactt agcattctct
     1681 cactaagggc tcgaggacca ttattaaatc ccaaaatcaa taactcagaa agcaatccgt
     1741 cattaccgcc actggatgaa aagcgcccat tagtagcatc atacacaata ctgacagttt
     1801 caccacctac tgtaatatct ccgcgcccat tggctgcaat tcgggcacga aaaacctcca
     1861 gcccaagatt aaatgaacgt gatccaatat ctgaggttaa tggcataaca ag
//
LOCUS       DAZGOB010000304         1879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGOB010000304 DAZGOB010000000
VERSION     DAZGOB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.306"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            140..772
                     /locus_tag="ACHLIF_005251"
     CDS             140..772
                     /locus_tag="ACHLIF_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9609051.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQY"
     gene            1106..>1879
                     /locus_tag="ACHLIF_005252"
     CDS             1106..>1879
                     /locus_tag="ACHLIF_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9609052.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADC"
BASE COUNT          634 a          340 c          346 g          559 t
ORIGIN      
        1 ttaaaatgtc ttaatcagaa ttatagcaaa tacagattaa tcgcaatttt tcccagcaaa
       61 attacaaaat catggtgtat tgacatcatc aatcaccaat gaaattatgc aattatatac
      121 ggatagggag gttcttaaca tggggctttg tagtcgttat aaaagtctta catgcaatag
      181 ttgctcaatg cattgccaaa taatgccaga agagtcaccg cgtttgcagt actgcgcaaa
      241 ctcgtgtttt tgtatgtggc ccgaagaaag ttcatatttt aatcgtggtg tggtagaagg
      301 tatcttaaca aaaaaccaca atgcgagatt aagcggatat atttttgtcg atttttcagt
      361 aagtttttta cgtctattcc ttgaaaaaga ctggattgac tatcttgcaa gtactgacat
      421 ggggattgtt ttagtcagcg accgcaatat gcaatcgtta gctaattact ggcgaaaaca
      481 caactcagca atatctgctg tcatatataa tgatgatggt cttgatgttg ccaatgaaaa
      541 gatcagacaa ctatttattg gtcgttattt atcatttacc agaggtaaca cattaactca
      601 gatggaattc accataatgg ggtatatggt ttctggttat aatccatatc agattgctga
      661 agttctggat atggatatcc gtagcatcta cgcgtacaag caacgaatcg aaaagagaat
      721 gggtggtaaa ataaacgaat tatttattcg ttcacattcg gtccaatatt gatactcaac
      781 gaccagccag aatcatactc tgtttatacg ggcatttatt gaaatgtccg tattttataa
      841 ataggccgtc ccctcaagag taaacaccat agtatttaaa accatcacac gaaaaattca
      901 gaagcattac gaaatatgaa tttacaaaca atagtggcat aaatgttaac catgttaatt
      961 tacgtaaagt tttacgttgc aacattaaag cctcatttca atcatcatga taaatataaa
     1021 attaatatat atttatgccg taaataccaa tatacttagc aaactatgtg atctccattt
     1081 cgattgattt agtgtttatt gacgtatgta ctggattatt aacgataata tcgagttctg
     1141 gcctgagcac cgaaaattaa tatcggtaca taacgccgat cttaacgtcg ttctgacaac
     1201 gccagccagt cgatgtttat cacttctact tgaagctttt cctgatgtgg ttgcacaaca
     1261 agattttttc accagagtct gggaagaaga aggtatgcgt gtgcctacta acacgttata
     1321 tcagaacata tccattatca gacgcggatt tcgcgctgtt ggtgatacta cccactcgct
     1381 aattgcaacc gtgccgagaa gaggattcaa gatccataat gacattaaca tacaaaatca
     1441 tgtaataaac tcgtcaacag acgcacatac acacaatgcc ccacctgcca taaaagttaa
     1501 tgcgggatac aaagagagca ttggtggcgc aaagaatttc aataacaaaa tcctcaaaca
     1561 tataaaaagt catctaatta tgttgagcgc atttgtcata ggcgcatatt ctgcatattg
     1621 gctatggaat aataatcaac cgaagccatt tttcaaggat tacaagactg ttgcggaaat
     1681 taatggctgc cattttaatg taacagaaga tacaattgac gggttgaaag agttcgataa
     1741 atataaaaca cggatactgg attcggggat taactgtaaa aaacatccgt ggttatactt
     1801 ccctcttgca aaatcctcac cggggatgat tgttatggca tgcaataaaa attataacca
     1861 acatgaagtg gcagactgt
//
LOCUS       DAZGOB010000305         1861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGOB010000305 DAZGOB010000000
VERSION     DAZGOB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.307"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            96..398
                     /locus_tag="ACHLIF_005253"
     CDS             96..398
                     /locus_tag="ACHLIF_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFO9609053.1"
                     /translation="MDAANLSPSGGVLPLAATINGNVSVDENTGVMIPENGVSRNIRI
                     AAGIALATTALAALGTGIAMACTTDASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
     gene            482..859
                     /gene="sctI"
                     /locus_tag="ACHLIF_005254"
     CDS             482..859
                     /gene="sctI"
                     /locus_tag="ACHLIF_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO9609054.1"
                     /translation="MNNINQSENINIHFNKVSQTNFVDDHTPLAPTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            861..1433
                     /gene="escJ"
                     /locus_tag="ACHLIF_005255"
     CDS             861..1433
                     /gene="escJ"
                     /locus_tag="ACHLIF_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO9609055.1"
                     /translation="MRKYIKNTFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNVTISVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1439..>1861
                     /gene="sepD"
                     /locus_tag="ACHLIF_005256"
     CDS             1439..>1861
                     /gene="sepD"
                     /locus_tag="ACHLIF_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO9609056.1"
                     /translation="MNNNNGIVKNDSDWLTALDFVKDVKGSPKHLTFHIYQKNAFLHD
                     FGSYWVLYIELNGDFRQVPTDTFIRLCNILAVSKEYKQMGIFQSNKKWYLCQLFNKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQASTSDTTMPPT"
BASE COUNT          642 a          345 c          351 g          523 t
ORIGIN      
        1 attgcaacta tttaaataat ataatagttg cccatgggat aatttggtta ttgataacca
       61 gacaaatgtc taaattagac aatggagaaa agttaatgga tgcagcaaat ttaagtcctt
      121 ctggcggagt attaccactg gcagccacaa tcaatggaaa tgtttcggtg gatgaaaata
      181 caggagtaat gataccagaa aatggggtaa gtcgtaatat cagaattgca gccggaattg
      241 cactcgctac tacagctctg gctgctttag gtactggtat cgcaatggca tgcacaacag
      301 atgcaagctc ccaagaattc ttaggtttag gtattgctac cggtgtatta ggtggtgtta
      361 ctgcgcttgg cggcggatta gcgatgaaat atgcctaatc atcgcatcgc ataaataaaa
      421 tagagaggtc atggatgcat tatgccatta cctctctcat gaaaagagac taaccgtaaa
      481 tatgaacaat attaatcaat cagaaaatat taatattcat tttaataaag tatcccaaac
      541 aaatttcgtt gatgaccata ccccgttagc accaacgccc tctgcggctg gagcggctca
      601 gtttcttgat caattacttc ctaaaacagc tggtgtatct tctccagagc aagttttaat
      661 tgaagaaatt aaaaagagac atcttgcaac aatgaacacc gatctcagtt tcgatgccct
      721 atctgcaggt ggactctcgc cagaagatgt gctcacatta caaaagaatg tgctcaacgc
      781 aaacgtcaat gttgatgtcg tatccaagtt agcaagtctt ctttcaacat cggttacaaa
      841 actggtttca atgcaataaa atgagaaaat acattaaaaa caccttttta tttgttttta
      901 tttgtttgac agttgcatgt aaagagcaat tgtatacagg attaacggaa aaagaagcca
      961 atcagatgca agcattgttg ttatccaatg atgtcaatgt atctaaagaa atggacaaaa
     1021 gtggaaatgt aacaataagt gtggaaaaag aggattttgt ccgtgcgata accattttaa
     1081 ataacaatgg cttcccgaaa aagaaatttg cagatattga agttattttt ccgcccagtc
     1141 agctagtggc atcgccatca caagaaaatg caaaaatcaa ctatctcaaa gaacaagata
     1201 tagaaaggtt actaagtaaa atccccggtg ttatcgattg cagcgtttca ttaaatgtta
     1261 acaataacga gagccaacca tcctcagcgg ctgttttagt cattagctcg ccagaggtta
     1321 acttagcacc atcagtcatt cagataaaaa acttagttaa gaatagcgta gacgatctca
     1381 aactagaaaa tattagtgtc gttatcaagt catcctcagg acaggacggg taatatgtat
     1441 gaacaataat aatggcattg ttaagaatga tagtgactgg ctaacggctc ttgattttgt
     1501 aaaagatgtc aaaggttcac caaagcatct tacttttcat atctatcaaa aaaatgcctt
     1561 cttacacgac tttggaagtt actgggttct gtatatcgag cttaatggcg attttcgcca
     1621 ggttccaaca gataccttca tcagactctg taatattctg gccgttagta aagaatataa
     1681 acaaatgggc atttttcaat ctaataaaaa atggtatctt tgccaactgt ttaataaaga
     1741 taataatcat cgcgcaaaca tgtcaaaagc aataatgcaa cacacactcg cctctttgct
     1801 tgacaagcaa tttgataagt tagagcaagc atctacatca gatacaacga tgcctccaac
     1861 a
//
LOCUS       DAZGOB010000306         1837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGOB010000306 DAZGOB010000000
VERSION     DAZGOB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.308"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            80..451
                     /locus_tag="ACHLIF_005257"
     CDS             80..451
                     /locus_tag="ACHLIF_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609057.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            472..663
                     /locus_tag="ACHLIF_005258"
     CDS             472..663
                     /locus_tag="ACHLIF_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO9609058.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            665..943
                     /locus_tag="ACHLIF_005259"
     CDS             665..943
                     /locus_tag="ACHLIF_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9609059.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            1230..1382
                     /locus_tag="ACHLIF_005260"
     CDS             1230..1382
                     /locus_tag="ACHLIF_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9609060.1"
                     /translation="MDTIDLGNNASLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LAKNTS"
BASE COUNT          552 a          392 c          435 g          458 t
ORIGIN      
        1 tttttttgca agtttagtgt ttaataaacc attaagtcaa gtatttgctt gtttagtgat
       61 tactaaagat aattaccaca tgcaaaaaaa agaaattcgc cgtttacgtc tcaaggagtg
      121 gtttaaagat aaaactctgc cacccaaaga gaagagctac ctatctcaac taatgagtgg
      181 gagagcctcg tttggagaaa aggctgccag aagaatagag caaacatacg ggatgccgga
      241 agggtatctg gatgcggaat acgcagaaca accgggggtt tctccaccac atgcagggtt
      301 aacgtctaat caactggaat tattgcagat tttttcagcc ttccctgagg atgagcaacg
      361 ccagataatc agcgagttaa agcagaaaaa agaatcaatg gaagatctca tagcgagatg
      421 gattgcggcg caaaaatgcc gccgcgcctg agttataaaa ccggaggaaa catgaataga
      481 gccctttcac caatggtttc tgaatttgaa accattgaac aagaaaacag ttacaacgaa
      541 tggctgcgtg cgaaagtagc aacgagcctt gcagatccgc gcccagcaat tccccatgac
      601 gaagttgagc gcagaatggc agaacgcttt gctaagatgc gcaaggaacg gagcaagcag
      661 taaaatgtta cccgtgttat ggcttgaaag cgcagatacc gacctagatg atataactag
      721 ttatattgct cgtttcgaca tagatgcggc tgaacgctta tggcagcgat taaggggttg
      781 tgtgctgccg ttatccgaac atccgtattt atacccacca agcgacagag tacctggctt
      841 gcgtgagatt gtagcccacc ctaactatat aattctatac cgcgtaacaa catcaagcgt
      901 tgaagtagta aacgtgatcc acgcaagacg ccagtttccc taactttcac taccaataga
      961 aacataacaa ccgcaacgac tttatcaaaa gcgttgtgtt tgttatgtcc cgcggtttag
     1021 tttttacttg acttaagttt aatgtttatt aaactaaaaa taccaccccg ccccacagaa
     1081 cgcagggaaa tacttcgagt tacccggcag tggtcagggg ttaagtagcc agcccgaggc
     1141 gtatgaacat gacggcaggg ttcaacttta ataactatgc agcaggtttt tgttccgcta
     1201 ccccggcgtt aaggggaaat gaggtcagca tggatactat cgatcttggc aacaacgcat
     1261 ctctggtgta cggcgtgttt ccaaaccagg acggcacgtt caccgcaatg acgtatacca
     1321 aaagcaaaac gtttaaaacc gaaaatggtg cccgtcgctg gcttgccaaa aataccagct
     1381 aaaccattta ttggattaat tcaatattct tgctgtaggg gtatagccga ggccaccaaa
     1441 gcccggaggt ggtgaaataa aaccgggcac aacacgaagg cgcatttccg gtatccataa
     1501 agagtcggtc ttgtctgtta aatttaaatg gtgggagtgc gcctccggtt gtgaataaca
     1561 acactgctgt gtgtagtctt ggcggcatca gttttttctt gaagttcgac tgatgtccgc
     1621 cctttttaaa gtgaattttg tgatgcggtg aatgcggcta agcgcacgcg gcacagttaa
     1681 aagtcatgtt agtccttatt ggtttgggtg ggaaagccga ctgtaattgt taactggttg
     1741 cagtcacctg gaggcaccag gcaccgcatc aacaaagttc atttgtaaaa atggagataa
     1801 ttatgattgc acatcacttc ggaactgatg aaatacc
//
LOCUS       DAZGOB010000307         1830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGOB010000307 DAZGOB010000000
VERSION     DAZGOB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.309"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..537
                     /locus_tag="ACHLIF_005261"
     CDS             <1..537
                     /locus_tag="ACHLIF_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO9609061.1"
                     /translation="KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRR
                     ATAFAISVINDKATIPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDK
                     NEEVRIEAIIGLSYRKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLY
                     KFDDNEIITSAIDKLKRS"
     gene            565..1523
                     /locus_tag="ACHLIF_005262"
                     /pseudo
     CDS             565..1523
                     /locus_tag="ACHLIF_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1535..>1830
                     /locus_tag="ACHLIF_005263"
     CDS             1535..>1830
                     /locus_tag="ACHLIF_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO9609062.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGV"
BASE COUNT          573 a          338 c          430 g          489 t
ORIGIN      
        1 aagagcgctt gcgttcgagc tacggcaatc gagtcaacgg ctcagcgatg caagaaaaac
       61 ccaatttatt cacctaaaat agtagaacaa tctcaaatta ctgcttttga taaatcgact
      121 aatgtcagac gtgctacagc atttgctatt tctgttatca atgataaagc aacaattcca
      181 ctattgatta atctgttaaa agatccaaat ggagatgtca gaaactgggc cgcatttgca
      241 ataaatatca ataaatatga taatagtgat attagggatt gttttgtgga gatgcttcag
      301 gataaaaatg aagaagtccg tattgaagca ataatcggac tttcctacag aaaagataaa
      361 agggttttat ctgttttatg cgatgagtta aaaaaaaata ctgtttatga tgatatcatt
      421 gaagctgcgg gtgaattagg tgataaaacg ctacttcctg ttttagatac tatgttgtac
      481 aagtttgatg acaatgaaat tataacttcc gctattgata agctgaagcg ttcatagcgt
      541 gagttgccta tgcacagtgg gggattcccg ccggcacggt gcagatgctc gaccggctgg
      601 aaagtgaaat cctggctgac cgggtgagtg aggaaagccg ccgctggctg gcatcgtgcg
      661 gcctgaccgt ggagcagatg aaaaaccaga tggacccggt gtacacgccg gcgcgaaaaa
      721 tccacctgta ccactgcgac catcgcggcc tgccgctggc gctcatcagc acggaagggg
      781 caacagcgtg gtgcgcagaa tatgatgaat ggggcaacct gctgagtgat gagaacccgc
      841 atcatctgca gcagctcatt cgcctgccgg gtcagcagta tgatgaggag tccggcctgt
      901 attacaaccg ccaccgctat tatgacccgc tgcaggggcg gtatatcact caggatccga
      961 ttgggctgaa ggggggatgg aatttttatc agtatccgtt gaatccggtc ataaatgtag
     1021 atccgcaagg tttggttgat ataaatttat accccgaaag tgatcttatc cattctgtag
     1081 ctgatgagat taatatccca ggcgttttca caatcggggg gcatggtacc cccacatcta
     1141 ttgaatccgc aacgcgcagt atcatgacag ctaaagatct agcatatcta attaaatttg
     1201 atgggaatta taaagatggg atgacagttt ggttattttc ttgtaataca ggtaaaggac
     1261 aaaattcatt tgctagccaa ttagctaaag agttacatac aaatgtaata ggacctgaca
     1321 cgctatggac gtggtggggg cgaggaacta atggtaagtt aaaaatggat acagtgctaa
     1381 cagcaccaac gaaccttaat tcaaataagg atctaatggc tataacaaca aaagaccttg
     1441 gtaattggat aacatatggg ccatctgggc accccatttc taatatgcaa ggtacgccag
     1501 aaaaacccag tgatataaga taggttgtag atgtatgaaa gcatgcttgt tactattttt
     1561 ttatttttct cttattggtc aattgcatgg tgctgatgtg aaaataaaag aaaacgaaag
     1621 tgtgatggga tctacagcaa tgacctatga tttaagtgaa gaaaagctga tgaaactgaa
     1681 gtataaatca caacatggtg attcagaggc atcatttcga ctatatcaat attactgctt
     1741 tactaaaaat aatattgata aacaattgcg attcttggaa agatcagcat ctcaggggaa
     1801 tgttacagcg cagtttaatt atggggtctt
//
LOCUS       DAZGOB010000308         1829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGOB010000308 DAZGOB010000000
VERSION     DAZGOB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.310"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..1774
                     /locus_tag="ACHLIF_005264"
     CDS             2..1774
                     /locus_tag="ACHLIF_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609063.1"
                     /translation="MIPNSTSNKGYVCIDMQCSSTSEPTASTSSNRSIKLAASTNVYP
                     ITRNDSELTLNDFLDNSSSTSSLDYINELGSQLTLNDFLDNIKTNEVDRTCTDVVINI
                     PQEIQTNTQENDLLLSDKNNSICIEIDERITKILTCKQKYQLDSIIHEIIPKENENAE
                     TVLHLMRVLNDQYHQVYNQSGCFYKAYMAIHNKIEQILPYAFRAGGGISIHLLIQALF
                     FNGDYNKSPSQSQQPSLYTSPSPTINTEAFLSNVLSLDITQVRILGDLLSATLFHAPT
                     IFYQYPKLIDEVKYCISNKKITGSVIARFTLCLTSTLLTMSPLLMLNGAVKTGSIVRT
                     IGRGVSYVDIPLALAILGDSWYKAYKHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLS
                     LMSSLSGAIMRSLEKGTPPQMMSLFIVNILNLLFHQNPYEGASASANALKRSTYSHNP
                     DILNTQAIALCVDLQHTKNITMPLFKTKDRISYAFNGQRTSPEDQKQILKEVINSCTQ
                     GERAILNTSQSETCKHKIDEIYEKRFSETELNTLPNEMKNFLIFLNKTHEKDISRLSM
                     GNEVNEKIIAVIVKTLAYRESMLC"
BASE COUNT          685 a          348 c          285 g          511 t
ORIGIN      
        1 cttgattcca aattcaactt caaataaagg ttatgtatgc attgacatgc agtgttcgtc
       61 aacctctgaa ccaacagctt ctacctccag caaccggagt attaaattag ccgcttccac
      121 aaatgtatat ccgataacaa gaaacgactc cgagctcact ctgaacgatt ttcttgataa
      181 tagctcttct acgtcatcat tggactacat taatgaattg ggttctcaac tgacgttaaa
      241 tgattttctt gacaacataa agacaaatga ggtggataga acatgtacgg atgtggtaat
      301 taatatccca caagagatac aaacaaatac acaggaaaat gatttgttat tatccgataa
      361 aaataattca atatgcattg aaatcgatga aagaattaca aaaatcctaa catgcaagca
      421 aaaatatcaa ctggacagca tcattcatga aattatacca aaagagaatg agaatgcaga
      481 aactgttctc catcttatga gagttctgaa cgatcaatat catcaggtat ataatcagtc
      541 aggatgtttt tataaagcct atatggccat acacaataaa atcgaacaga tacttccata
      601 tgcgttcaga gccggaggcg gaatcagcat tcacttgctc atacaggcat tattttttaa
      661 tggcgactat aacaaatcac cttcacagtc tcaacaacca tctttatata catcaccttc
      721 tccaacaata aatacagaag cattcttaag taatgtatta tcactagata taacccaggt
      781 acgcatactt ggtgatttac tatcagcaac tttatttcat gcaccaacaa tattctatca
      841 atatcctaaa ctaatagatg aagttaagta ttgtataagt aataaaaaaa taacaggttc
      901 ggttatagca cgatttactc tatgtttaac aagtacatta ctaaccatgt caccactgtt
      961 aatgcttaat ggagcagtta aaacaggtag catagtaaga actataggta ggggagtgag
     1021 ttatgttgat ataccattgg ccttagctat attaggtgac tcgtggtata aagcttataa
     1081 acatggttct tctgataacc caaattctgc tcagagattt atatcgcaag aagcggcctt
     1141 taaaaccacc cagcgggtat taacacaagg attaagtctg atgtcctctt tatcgggagc
     1201 aatcatgcgc tctcttgaga aaggcacacc accacaaatg atgtctttat tcatcgtaaa
     1261 catactaaat ctattatttc atcaaaatcc atatgaaggg gcatcagcaa gtgctaatgc
     1321 tttgaaaaga tcaacttact cccataatcc ggacatacta aatactcagg caatagctct
     1381 ttgtgttgac ctccagcata caaaaaatat aaccatgcca cttttcaaaa caaaagacag
     1441 gatatcttac gcattcaatg gacaaagaac atccccagaa gaccaaaaac aaatactgaa
     1501 agaagttata aactcctgta cccaaggaga aagagccatt ttaaatacat cacaatcaga
     1561 aacatgcaaa cataaaatcg atgagattta tgaaaaaaga ttctcagaaa cagaactaaa
     1621 tacattacca aacgaaatga aaaatttctt gatattttta aacaaaactc atgaaaaaga
     1681 tatttcgcgt ttaagcatgg gtaacgaagt taatgaaaaa ataattgcag ttatagtcaa
     1741 gacgttagca tatagagagt ctatgttgtg ttagacttta actctatatt gatataacat
     1801 tagccaaata ttcaacagtg tatgcgacc
//
LOCUS       DAZGOB010000309         1815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGOB010000309 DAZGOB010000000
VERSION     DAZGOB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.311"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..103
                     /locus_tag="ACHLIF_005265"
     CDS             <1..103
                     /locus_tag="ACHLIF_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFO9609064.1"
                     /translation="KPMPSREELLKRKSFGSVNDNKYLNAMWRSGKK"
     gene            100..657
                     /locus_tag="ACHLIF_005266"
     CDS             100..657
                     /locus_tag="ACHLIF_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609065.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVDETNDEYLVRKFGELESKLE
                     TALRECRSAGITIDNLEAKCAKMAAENSSLKQSEKEFNDFCREEFSEWEDDITETPAT
                     DAFLAEVKTEARKEGAYFVANRMLAAWEAGFIDDTAKNAADIARMILTSTEFMANAPE
                     GDFDRSFSDGVLEDIAEQLRKGVIQ"
     gene            654..1211
                     /locus_tag="ACHLIF_005267"
     CDS             654..1211
                     /locus_tag="ACHLIF_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9609066.1"
                     /translation="MSKIDYQALREAAERAIPAMERLLMLPVDDDLLTEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKRRDQKNEDIALTVGKLRVELEAVQKTS
                     AARIEAIDRTHKMFQREKDRADAAEKCIAELSASHSKLRDTMAGIHNTIRMDGGYTPL
                     AAILNAAKRAYEESASAAGIRIKGE"
     gene            1213..1350
                     /locus_tag="ACHLIF_005268"
                     /pseudo
     CDS             1213..1350
                     /locus_tag="ACHLIF_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          528 a          387 c          479 g          421 t
ORIGIN      
        1 aaagccaatg ccatcgagag aggaattgct taagagaaag agtttcggtt ctgttaatga
       61 caacaaatat ctgaatgcga tgtggcggag tgggaagaaa tgaaacaaat gacactaatt
      121 gagatggatg gttttctgaa aggtaaatgc atcccaagtg atttaaaggt tgacgaaaca
      181 aacgatgaat atcttgtccg taagttcggt gaacttgaat caaaactaga aacggcgttg
      241 cgggagtgtc gttctgctgg aatcacgatt gataaccttg aggctaaatg cgcgaagatg
      301 gctgctgaaa attcctcgct taagcaatct gagaaggaat ttaatgactt ttgtcgtgag
      361 gagtttagcg aatgggaaga tgatattact gaaaccccag ccaccgatgc ttttctggct
      421 gaagtgaaga ctgaagcacg caaggagggc gcttactttg tggcgaacag aatgctggct
      481 gcctgggaag ctggttttat tgatgatact gcgaagaacg ccgctgatat tgcccggatg
      541 attcttacct ctactgagtt tatggccaat gcgccggaag gcgattttga ccgttcattc
      601 tctgatggcg ttctcgaaga tatcgccgaa cagcttcgta aaggagtcat ccaatgagca
      661 agattgacta tcaggcactg cgtgaggcgg cagagcgtgc aattccggca atggaacgcc
      721 tgttaatgtt gccagttgat gatgatttgt taactgaaca ggaacttaag gattacggtg
      781 tggatattga tgcgctcaac gccttcaaat ttctgaccgg gccagaaacc gtgctggcac
      841 tactggatga acgggaaaga aaccagcaat acatcaaacg ccgcgaccag aagaacgagg
      901 atattgctct tacggttggg aagctgcgtg ttgagcttga agccgtgcag aagacatcag
      961 cggcacgaat cgaagctata gaccgcactc acaaaatgtt ccagcgtgaa aaagatcgag
     1021 ccgatgccgc agagaagtgc atagcagaac tgtctgctag ccacagcaaa ttgcgcgaca
     1081 caatggctgg catccacaac acaatccgaa tggatggcgg ctatacgcca ctggcagcaa
     1141 tccttaacgc tgctaaacgc gcatatgaag aatcagcaag cgcagctggc attcgcatca
     1201 aaggagagtg atatgagcac tatcactaaa gaacgtatcg aattgttcat taaatccccg
     1261 cttgataacg gacttactag tggcgaacaa atggaactgg cacggattgc tctggcatcg
     1321 ctggaagcaa aaccaatagg tgcattccac attgcagaac agcaagttga cggcacaagt
     1381 gactacctca aggatggaga atggcctatt gataatggaa ttattgaggt ctacgccgct
     1441 ccgccagtac cggtagtacc tgctgcatta cctgagaacg acgatgagga cgggcatgac
     1501 attgattatc ttgatccatc tgaagtttac gcgcttgggc gaacagctgg ctggaacgcc
     1561 tgccgcgccg ccatgcttca gggtaaatca gaacaaccac aaaacgcaca acaaaatatt
     1621 ccggaaaata ttcccggtgg caactctccg gtaactccgg atggttgggt tatggtgccg
     1681 aaggagttaa ccccggaaat gatgagagcc gttcagatta gaagtgagct tggagggtat
     1741 gctacttcca atctatctgg tgcatacaat atgttttctg agttctggaa tgttgctgtc
     1801 tcagctgcgc ctaag
//
LOCUS       DAZGOB010000310         1813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGOB010000310 DAZGOB010000000
VERSION     DAZGOB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.312"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1813)
                     /locus_tag="ACHLIF_005269"
     CDS             complement(<1..>1813)
                     /locus_tag="ACHLIF_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFO9609067.1"
                     /translation="TNDLKSLDNVEIGGIPAKKYLEHGLFSEYRQDGTIPYVVSTLNI
                     SGPDMIMRQMKKYYKSLGRIGEVHIKDNKLSDMNFMGVYASSDKENKSFNWLNPVSVG
                     VNDITPDDESSWAVRNNDINKILFEKINCHVPEKLPTSLYYEIDSRVFFHGWDNKSIQ
                     YVTEINKDLIKDINLLLTSSNVDVKLLIKLDRELYAISSKIENPLALRSIRTLQLQLT
                     NYVTSNTFEPENTINFIYDFYSKKQNDLLSAIKLFSRNDVETKIIVWYNSTMEKNVFL
                     REVISCVLWTKKVDSYIKENKKHLSTEDAEALRDYAKLKIKELFSMLDDDGYKRIITT
                     NSYIKERDKLSGIIHNIENSIISGHESSDIIRSHQHEWGDLSTVEQFKKFEFYVKSEL
                     SFSKSIFDDIKTKYITDPETKRNALYHQLDSDIKERIAFLDISHYAYPGSLLEKLQLS
                     GYVFSDINIIAEYLLSSYGISGHYSHGVVYPAPSDKLFELLRRHTNSNSDWIEKIIPY
                     VYDILSGNVSSFLHPPLSEEQKKILSDIKLEISESVSEQYFMKLTEQKSSVIGIKYSV
                     DFDRYNENLFLSLPINQNLTLPFMYRYFEMLYDIHIGI"
BASE COUNT          569 a          290 c          263 g          691 t
ORIGIN      
        1 agatcccaat gtgaatgtca tataacatct cgaagtagcg atacatgaaa ggtagagtta
       61 aattttgatt tataggtaaa gacaaaaata aattctcatt gtatctatca aaatcaacag
      121 agtatttaat gccaataact gatgattttt gttctgtcaa cttcatgaaa tactgttctg
      181 atacagattc actaatttct aattttatat cactcaatat ttttttctgt tcttctgata
      241 agggagggtg caagaagctg gacacattac ctgataagat atcataaaca tatgggatta
      301 ttttttctat ccaatcagaa ttggaatttg tatgtcttct taataactca aataatttat
      361 cagatggagc tgggtatacc acgccgtgag aataatgccc agaaattccg taactagaca
      421 ataagtattc tgcaattatg ttgatgtcag aaaaaacgta gccgcttaac tgtaattttt
      481 ctaataaact acctggatat gcgtagtggg atatgtcgag aaaggctatt cgctctttga
      541 tatcactatc taattgatgg taaagtgcgt ttcgttttgt ttcgggatct gttatatatt
      601 tggtttttat atcatcaaaa attgacttgg aaaatgataa ctcactttta acataaaatt
      661 caaatttttt aaattgctct accgttgata aatctcccca ctcatgttga tggcttcgta
      721 ttatatcaga tgattcatga cctgatataa tagaattttc gatgttgtgt ataatgcctg
      781 atagtttatc cctctcttta atatatgaat ttgttgttat gattctttta taaccatcat
      841 catccaacat cgaaaatagt tcttttattt ttaattttgc ataatctctt aaagcttcag
      901 catcttccgt agataagtgt tttttgtttt cttttatata agagtctact tttttagtcc
      961 atagaacaca tgatataacc tctcggagga atacattttt ttccattgtt gaattgtacc
     1021 aaacaatgat ttttgtctcg acatcattac gcgaaaataa cttaattgcg ctaagtaaat
     1081 cattttgttt tttactataa aaatcataaa taaaattaat tgtattttca ggttcaaatg
     1141 tgtttgaggt tacataatta gttaattgca gctgtaaagt ccttatagag cgcaaggcta
     1201 acggattctc tattttgctg cttattgcat ataattcacg atctagcttt attaaaagtt
     1261 taacatcaac attagacgat gttaacaata aatttatatc tttaataagg tctttattaa
     1321 tctcagtaac atattgtatg cttttattat cccatccatg aaaaaaaact cgcgagtcga
     1381 tttcatagta tagggaggta ggtagttttt cgggtacatg acagtttatt ttttcgaata
     1441 aaattttatt aatatcatta tttctaacag cccatgagct ttcatcatcc ggtgtaatat
     1501 cattaacacc aacactcact ggattcagcc agttaaaact tttattttct ttatctgaac
     1561 ttgcatatac ccccataaaa ttcatatcac ttaatttatt gtctttaata tgtacttcac
     1621 ctatgcgccc tagagattta tagtattttt tcatttgtcg cataatcatg tctgggccag
     1681 atatatttag agtacttact acataaggaa ttgtaccgtc ctgtcgatat tcagaaaaca
     1741 aaccgtgttc taaatatttt tttgcaggta ttcctcctat ttccacgtta tctaaagatt
     1801 ttagatcatt tgt
//
LOCUS       DAZGOB010000311         1809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGOB010000311 DAZGOB010000000
VERSION     DAZGOB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.313"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..918
                     /locus_tag="ACHLIF_005270"
     CDS             1..918
                     /locus_tag="ACHLIF_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HFO9609068.1"
                     /translation="MNLLNSQLNMKINDFYLPLLSVIGTGRLYITPEGHACHAYFREV
                     SGNGIRFTLTCSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDVYPVIENNNGENRLNVIILEITVQSLQYLINENWQFVPHS
                     NTIFFDGYIAPGWTDYPVTELTVGDSLRLYHVDDSKERECWLVINNPLATVKLCDNNL
                     FIADVQAADLLCTISNEAVMDRIYCVIGTIHVDIHMLRNAKKDDIIDSTGYHLFGGCR
                     LIRNNTTIAYGSIVKINEDFYFTVSLVCD"
     gene            949..1464
                     /locus_tag="ACHLIF_005271"
     CDS             949..1464
                     /locus_tag="ACHLIF_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HFO9609069.1"
                     /translation="MSSSLSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKAR
                     TQQRHHRVYSEMADYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSF
                     LSGGVTKALESKLNERHITPLSPTWFPRTPLEGVLTERGLSSLLRRVQSTENLDYSNA
                     MISNSFSNSVV"
     gene            complement(1683..>1809)
                     /locus_tag="ACHLIF_005272"
     CDS             complement(1683..>1809)
                     /locus_tag="ACHLIF_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HFO9609070.1"
                     /translation="LKEDSVLLNIAFPADKVNITEFINLMENGYLLKNEVISLLS"
BASE COUNT          552 a          292 c          368 g          597 t
ORIGIN      
        1 atgaacctat tgaattctca attgaatatg aaaattaatg atttttactt gccattgttg
       61 tcagtcatag gaaccggtcg cctctatatt acgcctgaag ggcatgcttg ccatgcatat
      121 tttcgcgaag tcagcggtaa tggtatccgc ttcacgttaa catgctcagg ctatgagggg
      181 cgtttttgga tatcagaaga acagttcatt cagtggtgcc aggagttatt cccgtatagc
      241 gaaagccggc tgataccgga agatatgata aaactcatga tcttatgggt aatgcagact
      301 gctttaccag aaggtgatgt gtctgttgat gatgttcaat ttacgatgct aaataaagat
      361 gtttatccgg taatagaaaa taataatgga gaaaacagac ttaatgtaat tatactcgaa
      421 ataacggtac agagtttaca atatctaatt aatgaaaatt ggcaatttgt tccacattct
      481 aataccattt tctttgatgg atatattgct cctgggtgga ctgattaccc ggttacagag
      541 ctgactgttg gtgatagttt aaggctctat catgttgatg acagcaaaga aagagagtgt
      601 tggcttgtta ttaataaccc tttagcaacc gtaaaacttt gtgataataa tttatttatt
      661 gcggatgtcc aggctgcaga tttgttatgt actatcagca atgaggctgt aatggatcgc
      721 atttattgtg ttattggaac aattcatgtt gatatacata tgttgcgcaa tgccaaaaaa
      781 gatgatatta tcgattcaac tggatatcat ctttttggtg ggtgccggtt aatacgcaat
      841 aatacgacta tagcatacgg aagcattgta aaaatcaacg aagattttta ttttactgtt
      901 agtttagtat gtgattaatc aggaataagt tatataggga gatgtattat gtcgtcatcg
      961 ttatcaggaa taatactcaa tacttcgctg acaagtcacc atatgtcatg gggcaagttt
     1021 agcagaactc ttcatgtatt gaatgaagat atcgtagatg aaattcagct taaagcccga
     1081 acacaacaac gccaccatcg agtatattca gaaatggcgg attactccgt tgtctccttt
     1141 cggggcaaag aatattctgt tcgttttatt aaagattaca aaacagataa ttatgtgtac
     1201 aaagtacaaa agatgactca ggaaagtggg tgtctctcaa gattatttag ctttctttct
     1261 ggcggtgtga ctaaggcttt ggaaagtaaa ctaaatgaga ggcatatcac tccactttct
     1321 ccaacctggt ttcctcgaac tccactggag ggggtcttaa cggaaagagg attatcttct
     1381 ttgttacgtc gcgtacagtc tactgaaaac cttgattata gtaatgctat gatatcaaac
     1441 tctttctcta actccgtagt gtgattatca tttgagcttt cctcctggtt taactccaga
     1501 ataattgtga ttctggagtt tttctgtttg ttttatgtcg ataatacatt gtttttagga
     1561 aaatatggga tgaatattca gagtgagttt ggctggcaga agtcagagat tctgtgatga
     1621 ggaggggatt gagctgaatg gatgaattcc ttccagctca taatttatca ggataaaaca
     1681 tcttacgaca ataaactaat tacttcattc tttaataaat aaccattttc cattaaattg
     1741 ataaactctg taatgttaac cttatctgca gggaatgcaa tattcaaaag tacgctatct
     1801 tcttttaat
//
LOCUS       DAZGOB010000312         1804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGOB010000312 DAZGOB010000000
VERSION     DAZGOB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.314"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            214..1566
                     /locus_tag="ACHLIF_005273"
                     /pseudo
     CDS             214..1566
                     /locus_tag="ACHLIF_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1579..>1804
                     /locus_tag="ACHLIF_005274"
     CDS             1579..>1804
                     /locus_tag="ACHLIF_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO9609071.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQ"
BASE COUNT          600 a          351 c          336 g          517 t
ORIGIN      
        1 atttcagaat gaaattaact gtgatgatta ttaattcact acagaggaat aaaaaacaat
       61 ctgaagcatg ctctgaaaca tcctactctg tcactctaat gatggcacct tgctcttctt
      121 ataaagaagg gaacaattaa gaatattaaa tcttcaaact ttcatcaact tcacatcaag
      181 aaattaaaaa atttaaacat taatctaaat aataacaacc tgatcacata atcgggtacg
      241 attttatccg ataactataa tgctataact ctgggaaatg gttggaatac accgctgggt
      301 gccatctctt tttacgctgc ccgatcgagt agtaagttaa ataatgatac cagacacgaa
      361 ggtacgagct atcaggtcgc ctataataaa tatctagtac aaacagccac tcgatttagc
      421 gtggccgcct ggcgttatgc ttcgcaggat tacaggacat tcagcgacca tctttacgaa
      481 aatgataaac actatcatca gagtgattat gatgattttt atgatattgg cagaaaaaat
      541 agcctttctg ctaatattat gcaaccttta tccaataatc tgggaaatgt atcattaagt
      601 gcgctttggc ggaattactg ggggcgaagt ggaaatgcta aagattacca attcagttac
      661 tccaataact ggcaacacat tagttatact ttctctgcca gccaatctta tgatgaaaat
      721 aataaagaag aggagcgttt taatctgttt atctccattc ctttctactg gggggatgat
      781 attgccaaaa cacgtcacca aattaactta tcgaattcga cctcattttc caaagatggc
      841 tattcctcca acaatactgg aattactggc atagccggtg aacatgatca gttaaattat
      901 ggtatatatg ttaatcagca acaacaaaat aatgatacct cgcttggtac gaatttaagc
      961 tggagaactc ccatcgccat aatagatggc agctatagtc attctaaaaa cgcctggcaa
     1021 agtggtggaa gtattagtag tggattagtt gtctggcccg gtggtattaa tatcactaac
     1081 cagttatccg atacatttgc aattctggat gcgcctgggt tagaaggtgc acatattaat
     1141 ggacaaaaat acaatcgaac aaacagcaaa ggccaggttg tttacgacct gataatacct
     1201 catcgtgaaa accatctggt acttgatata gcaaacagtg aaagtgaaac agaattgcag
     1261 ggcaatcgtc aaattattgc accttaccgt ggagcagttt cttatgtgca gtttacaact
     1321 gaccaacgta agccctggta tatacaggca ctgcgtcccg atggttcgcc attaaccttt
     1381 ggctatgacg tactggatct ccaggaaaat aatattggag tcgttggcca gggtagtcgc
     1441 ctttttattc gcgtagatga aattcccact ggcataaaag ttgctctcaa tgatgaacag
     1501 aacttattct gtactattac ttttcaacac gttatcgatg aaaacaaaac atatatatgc
     1561 cagtaaaagg agtttactat gaagtggctt ttattaatca ccttgagtct atattctttt
     1621 attgtgcaaa gtgctccctg tgcattgacc aatgtcggtg aacaacgtgg aacatatata
     1681 ctcaagccac tgagtatgaa aggtaatcta accgccgaaa aaattgaaaa caccggaaat
     1741 atcattgagt gtacagactt gcaagagaca accaacgcca ttaaagttca ttttttaaca
     1801 caga
//
LOCUS       DAZGOB010000313         1790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGOB010000313 DAZGOB010000000
VERSION     DAZGOB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.315"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(190..300)
                     /locus_tag="ACHLIF_005275"
     CDS             complement(190..300)
                     /locus_tag="ACHLIF_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609072.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHANTIKLIFEM"
     gene            complement(353..757)
                     /locus_tag="ACHLIF_005276"
     CDS             complement(353..757)
                     /locus_tag="ACHLIF_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO9609073.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(978..1709)
                     /gene="bluR"
                     /locus_tag="ACHLIF_005277"
     CDS             complement(978..1709)
                     /gene="bluR"
                     /locus_tag="ACHLIF_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO9609074.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          498 a          376 c          375 g          541 t
ORIGIN      
        1 atgagaataa ggtttcaatt tttgagttat ataggaaagg tttaacctgt tcctggctaa
       61 aatacatata actggatgat gactaaacca aaacacatgt gcgttaagta ttgaaacggg
      121 cgtatggcac acccgttgtt tttataaata tattaaccgt tataaaataa cgtatagaaa
      181 gtcaagtgat cacatttcaa atatcaattt gatagtattg gcatggtgat tatttatggg
      241 tagcaataaa aggacagtat ttatcatcca tagggatagt ctctgtactt ttattcccat
      301 tatgctaatg ccttactgaa ttatgaagca tttcttaagc atccaacttt agctagatta
      361 atggtttatt attttctaca tcttcaatat ataaaagcgt attatcaatg gcgtagtaac
      421 tgcgtttgtt atgattaaca tcagtaaccc accggaaaac gcccgcgcct gctagtgttg
      481 aacagtattc ccgaaatgta gattttccgc aaatatgaag caatgcggcc tcttttattt
      541 tagcagggtt cttggtcgta ctaactttta acaggttcct ggttcctctt aataacaaaa
      601 ccgtgtcatc gtgagtaata attctgatgt tatccgtagc cagataataa atgtaatgtg
      661 caatacggtg atgttttaat tctgaataaa accaggagaa gttttgctct tttctcactt
      721 gctcaaacat cttttgaaaa acaacgacct gatccatcag gataataacc tcttgttagt
      781 tgtgagactg cgtagtgtgc acgatcggtt ttaccacttc aatctggtct gtcctttggc
      841 tgtgatatgt acagagtgtg atagagggaa tatctgaatt ctcccggtga gcattttgca
      901 acggaccagc tccggtacaa acgctgttgt gggttcagat tataacattc tgtctaaggg
      961 gcgggataaa ggtgaaatta gggggcatga aagatgactt tataaccttg ctcaccccaa
     1021 tgttgtaaaa gttcgttttg ccttctcgtt ggtgccatgc ctgtccagac aatcaatgtt
     1081 tgcgtcggga acagttccgg gcgcggcgat tcaatgggat cagcaagaac agaaatgtgc
     1141 catcctgaca atgataaacg ccatgcttcc agccacaatc gtgctctctc ttcaacattc
     1201 cacgccatca gaatggcttc tttaccgggc ttacggcgca tttcgaaaag cgaggttgct
     1261 gcgtattcaa ttaatgcgcc gtcaaacata ctgctcataa tgcgggaggt gttgtgatca
     1321 agcacgagac gctggcgaac aggaaggtaa acatgattaa tcaattgatc tactgggtac
     1381 tctcgaccca gtgaaataat tctcgcgcgt agtttggcag gattagccat gcgaagaatt
     1441 gacatcatct cttcttgcag gcggctccag tcatcttccg tatcctggct ggtggtttcc
     1501 agtaatgctt taactttgcc tacagggacg ccattactta tccaacgctt gatctcttcg
     1561 atgcgttgta tgtcttcttc atcaaagagt cggtgtccgc cttcactgcg ctgtggtttt
     1621 aacaaaccgt agcggcgttg ccaggcccgg agagtgacag gattaatccc gcaacgttca
     1681 gcaacatcac caatgctgta ataagccaca attcctcctt gcggtcacaa atctccgtcg
     1741 cctgtacacg acccaataat actttgtaca atatacgcta aaattgtaca
//
LOCUS       DAZGOB010000314         1787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGOB010000314 DAZGOB010000000
VERSION     DAZGOB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.316"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1412)
                     /locus_tag="ACHLIF_005278"
     CDS             complement(<1..1412)
                     /locus_tag="ACHLIF_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9609075.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEA"
     gene            1389..1531
                     /locus_tag="ACHLIF_005279"
                     /pseudo
     CDS             1389..1531
                     /locus_tag="ACHLIF_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1577..>1787
                     /locus_tag="ACHLIF_005280"
     CDS             1577..>1787
                     /locus_tag="ACHLIF_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609076.1"
                     /translation="MAKHGAGCLPVNSVSAPESAIIPYTWLLIAPPHRGIHHAEVFLI
                     NSKQKNQALCRLFLFITGHSNTTMPQ"
BASE COUNT          397 a          539 c          431 g          420 t
ORIGIN      
        1 gcctccttgg tcagcttcac tgccttacct ccggttccct ccgcatcgct gaccacttct
       61 gccctgccgc cactggctga ccatccctgt actttcaggc ttccctctga ctcgctggca
      121 aggtaagaga gcagtgttgt gacgcctgtg gcttctgcac cggaaggcga tgaagggcgc
      181 acctctgata ctgtcgatga tgcccccgcg tttagcgcca ctcttcccgc atggcgcaaa
      241 atcgccgttg ccagacggtc ggaaataatc ccacggcgtg cccaggcgct gaaatggctc
      301 gccctgtcct gtgacgtcca ggtggctgag ctgtcacgcc atttcgaacc gtaatatccg
      361 atacccggaa tgtccgggtc ttcttccggt ttgttcgtcg gcacattcac cccgttctca
      421 tcggtcatga acggtacgaa atggatattc ttttccgttt tgtttttata gctgccgtac
      481 accgtctggt acgtggattc gttcttctgc ttccagaaat acgtcgtatc tccacatatc
      541 cagggaacac cgccagcaga gccaccgacg cactgacctg ccatatccgc caggtctgca
      601 cggaatttat caaccagcgc accaaactgt gctgcgtgat ttgccggcgt accgccaaaa
      661 tcaaattccc cctgcatcca caccacggca aacagcacat ttttcggatt cttcttcagt
      721 gctgcttttg ttcgaccgat aaggtcctta tacagcggct tgtccacacc ccagcgggtt
      781 gaattctccg aggcaccact cgcgtcactg tatgtgccat cggctccggt ggtgaacgct
      841 gaaccaccac gacagcacgg aaccagcaga atgcccgcat tcgccggtat aaacggcagc
      901 aattttttgg cgatatgcag cccttgcccc acggttccgt actgtccctt tgacaggtcc
      961 gctttcggat ggttaaggcg gctcatgtcc tgcacatcat gcagacaatg gtccgccgga
     1021 atgatgtcgt tatatttaca ggcgacaccg cccggtgtca ccgtactgcg acgcgccagc
     1081 tgcttaatgc gcgggtccgg acggtcatat gtctccggca gcggaaggcc ttcaccatat
     1141 gccatgctgt ttgactgccc cgccagaacc acaacaaagt aatactccgg gtctctggtg
     1201 gcgctgatta ctgtgccttc tccatccgac ggcttcacca acacaggtgt ggtgacatca
     1261 ccttccgccg caatggcctg catcagagta taaggcgtga tggccaccgg gctgccaaat
     1321 ggctgccacc cctccttcag tttttgtgtc agcttttccg caaggtctga cggcgacgcc
     1381 gccctgacaa catcataatg tttaatcgac atcgaatttc tcccgtgtag aggaacagag
     1441 ttaaaaagcc ggaagcggaa tcaaatcaca ggatggccat ctgccagtgg ctggtcgtaa
     1501 aaaaaggcca cgccatgcgc agccggaaat aaagggataa cgatgatagt ttgagaaaaa
     1561 cagaaacaac acttttgtgg caaagcatgg tgccgggtgc ctcccggtga attcagtatc
     1621 agcacctgaa tccgcgatta tcccatatac ctggttgctg atcgcccctc cgcacagggg
     1681 gattcaccat gcagaagtgt ttttaataaa cagcaaacaa aaaaatcaag cattatgcag
     1741 gctgtttctt tttatcaccg gccacagcaa taccacaatg ccgcaga
//
LOCUS       DAZGOB010000315         1779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGOB010000315 DAZGOB010000000
VERSION     DAZGOB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.317"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..438)
                     /locus_tag="ACHLIF_005281"
     CDS             complement(<1..438)
                     /locus_tag="ACHLIF_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFO9609077.1"
                     /translation="MLFVLILSHRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWADVEPSLQEFFCDNGEEWVHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTD"
     gene            complement(451..873)
                     /locus_tag="ACHLIF_005282"
     CDS             complement(451..873)
                     /locus_tag="ACHLIF_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9609078.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKTQREKIQKLIPAILAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(870..1124)
                     /locus_tag="ACHLIF_005283"
     CDS             complement(870..1124)
                     /locus_tag="ACHLIF_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9609079.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADVILTIGRNKTTTKKLMGVDS"
     gene            1204..1623
                     /locus_tag="ACHLIF_005284"
     CDS             1204..1623
                     /locus_tag="ACHLIF_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9609080.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
BASE COUNT          493 a          424 c          364 g          498 t
ORIGIN      
        1 atctgtttga acacctgtta aatttctctc ttgttttgtt tgaacatctg cttcctttct
       61 gcttcttctg gcctgaacag atgcttttcc tgcggctgat tttttggtta atttttccct
      121 gactgatgcc agatcttcct caatccgaag atgcacccat tcctcgccgt tatcgcaaaa
      181 aaactcctgc aaggatggtt caacatcagc ccatcgctcg ttagtcagac gggcaatttt
      241 tgccagcctg tttttaggta ttggctttcc tgtttgccag taattgaaca tcagcaacaa
      301 atacgcacca tgctcctctg ctgacaaatg catggtgtca gccaggtaat cagctatgta
      361 cagttgcatg tatggtaatg cggccataat tgccccgtat gatgctgccc ggtggcttag
      421 aataagcaca aacagcatgg aaacttttgc ttaatgaaca atgacagaat cgtcggaaga
      481 cccgccgccg ctgaaatgcg ctttccggta aacggcttgg actgcatcat catgcgcatc
      541 aattgccgta cttaacgctt cctgcgccgc cagtaatgca cggcgttcca gggtatcgaa
      601 gatgcagagt cggtgacgca gctcgcgcgg aaggattgcc agaattgctg ggatcagctt
      661 ctgaattttt tctctttgcg ttttcgtttc acctttcaac caacggtgat agatattctg
      721 ctgattattc cagtccttgc ctggtaccag gggcaattcg ccgcccccct ggcgcagata
      781 ttcttcagta atggcattgg ctacccatgc ctgccctttt tcggctgcca gggctaacaa
      841 cactgactcg atgtgctcat gcttgatttt catgaatcaa ctcccatcag ctttttcgta
      901 gtagttttat ttctgccaat agttaaaatt acatcagcag aaaataatcc gtttgatgca
      961 tgagcgattt tttcagcgta atttgtttcg ccggtatatt ctgtgcgagg caattttccg
     1021 ttatccatcc atttgtagat tgctctttgg ctgacaccac aaacgtcggc cacaacagaa
     1081 acgcgaacag ttttgattac atcttcaagt gttttctggt tcatatcatc ctcacaatgt
     1141 gaactttgag tacatgctat aacagaactg acagtacatt caagagcgaa tatcattgaa
     1201 cttatggttc atgaagataa agcgcgtaaa gagttcgcca gtaggcttgc gctagcctgt
     1261 gaaaacgctg gttatgaaca acatggaagg caggcagaaa ttgcccgtcg aatgaaatta
     1321 acaccaaaag cggttagcaa atggtttaat ggcgaaacaa ttcctcgccg agagaaatta
     1381 agggaattag caacactcat tggaacaaca ccaacctatc ttttgggaga ggatacagaa
     1441 gaaagtggac aggtacgttt ctatcaggag ttaaatccaa gacaaaaaat catcattgac
     1501 cttctggacg agctccctga cagtgagaca gatgaacttt taaaaactct tgaggagaaa
     1561 aaacagaagt acaatgcaat ttacgaagag ctagcacgaa agaaaaaaca aaaagcctct
     1621 taaaccagca taaatccggt agcgtccccc tccgggtttg tgcttcactt tatcccatct
     1681 cattttttta cacacaaaat gtacttaaag tactttacat caatgaacac aaagtacatt
     1741 atatacctac caacccaccc cgccccacag aacgcaggg
//
LOCUS       DAZGOB010000316         1750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGOB010000316 DAZGOB010000000
VERSION     DAZGOB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.318"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..518)
                     /locus_tag="ACHLIF_005285"
     CDS             complement(<1..518)
                     /locus_tag="ACHLIF_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9609081.1"
                     /translation="MRKLYAAILSAAICLAVSGASAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDIRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVSYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVA"
     gene            complement(589..>1750)
                     /locus_tag="ACHLIF_005286"
     CDS             complement(589..>1750)
                     /locus_tag="ACHLIF_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9609082.1"
                     /translation="RITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTV
                     GKSAFVEGVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWK
                     DANDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGN
                     ETPMFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGRVNAN
                     AGTLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTIS
                     DDHNFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAI
                     PGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          399 a          498 c          394 g          459 t
ORIGIN      
        1 gccacggatt cggtcgggtt aaactgcacg ccagctcccc acgccagaga cgtgttgctg
       61 tggcgaccgt catcacttcc ggtcagcacg tcgtgcgttt tccccttgtt gtcagttacg
      121 cggagataat ccccggagaa agtcgacaca cggctgtaag acacacccgc catcgcatac
      181 gcgctgaacc attcattcac gcgcacagac ggccccgcca tcacgctgaa ccagcggtta
      241 cgcacggaat cttcatgcca gcggatatcg ctgtaacggg taagctggcg attcttgtct
      301 cctgcatagc tgaatgacgt caccagcccc agtgtgtccg taaactcata acggtatttc
      361 acgttaatcc cgttcagatc atcgctgccg ggaacgttcg tcgaggcatg aagatacccc
      421 gcgctcagcg tggactgatg ttcagatgcc catgcagacg caccggatac ggccagacaa
      481 atagctgcgg acaaaatggc tgcataaagt ttacgcataa ttacctctcg cttttctgca
      541 ataaaaaagg cgtcattcct gacgcccttt attggggtta taaatatttc aacgaatact
      601 gataccggaa gccgcttttt tggtcacaat caccgtacag tcggtgatat tccctgcccc
      661 ctgattgcct ttctggaaaa tcttaaactc cagagtgacg ctaccaccac cactaggcat
      721 atcaataact gcactgtaac taccgggaat ggccccttta gtttctctgg atgcgattaa
      781 tacaccgttt ttgcgaactt caaaaccata acccgtgtat cttgtacctc ccgggttatt
      841 accacttccc ggatcgctat acgctattcc gttaaagata atgggcggaa taatgatttg
      901 acggtcaaag ttatgatcat cgctgatggt gactgtaacc gtcccgtttg gtgtttccgt
      961 attaccccac gtaccagcct gtttcgggaa tgatttggat acagctttaa cgaagtcacc
     1021 tctgacctga gtcgcctcca gcatgccctt aatcgtacag ttttcattta ccgtgacatt
     1081 gttgagcgtc ccggcgttcg cattcactct gccactgata tccgcatttt tagcggtcag
     1141 ctttccgtct gatgtcaggg aaaataccgg aggactgcca ccgctggtaa tggtgggggc
     1201 tgtcaggcgc ttcaggaaca cgtcgttcat gaatatctgg ttgccctgcg ccacaaacat
     1261 cggcgtttca ttcccgtttg ccgggtcaat aaacgcgata cggttagcgg caaccagaaa
     1321 ctggctcagc ttgccttcct ccgtgtcctc catgctgagg ccaatacccg cgacataatg
     1381 tttgccgtct ttggtctgct caattttgac gccccacatg gcattccatt tatcgttagc
     1441 gtccttccac tctttcgaaa actcatccag tctgctggcg ttatcctccg tcaaatcaac
     1501 tttttccagc agctctttac cgagatggga ttcggttatc ttgcctttga aaaaatccag
     1561 gtaaccttcc gcatcatcgc tcgcccgacc gacgccctcc acgaatgccg atttgccaac
     1621 ggtgttcaca ctgcggatat aaaagtaata atcatggccc ggtttgatat tgatactggc
     1681 ggctatccag tacagcgccg taccaagata gcgggctgtg gtttcaacct gcctgatatc
     1741 ggtaatccgc
//
LOCUS       DAZGOB010000317         1735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGOB010000317 DAZGOB010000000
VERSION     DAZGOB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.319"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1735
                     /locus_tag="ACHLIF_005287"
     CDS             <1..>1735
                     /locus_tag="ACHLIF_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO9609083.1"
                     /translation="ASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAAAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAETPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTLTGFTNFAWV"
BASE COUNT          475 a          493 c          503 g          264 t
ORIGIN      
        1 acgcgtccgc ggtggcacag aacacggcag ccgcgaagaa gtcagccagc gatgccagca
       61 catcagcccg tgaggcggca acccatgcga ctgatgctgc aggctcagca cgcgcagcca
      121 gcacgtcagc cggacaggcc gcgtcgtcgg ctcagtcagc gtcttccagc gcaggaacgg
      181 catcaacaaa ggccactgaa gcatcaaaaa gtgctgccgc tgcagagtcc tcaaaaagcg
      241 cggcggccac cagtgccgct gcggcgaaaa cgtcagaaac gaatgcggca gcgtcacaac
      301 aatcagcagc cacttctgca tccaccgcga ccacgaaagc gtcagaagct gccacctcag
      361 cccgggatgc gtcggcttca aaagaagcgg caaaatcatc agaaacgaac gcatcatcaa
      421 gcgccagtag tgccgcttcc tcggcaacgg cggcaggaaa ttccgcgaag gcggcaaaaa
      481 cgtccgagac gaacgctaag tcttctgaga cggcagcggg acagagcgcc tcagctgcgg
      541 caggctcaaa aacagcggct gcgtcgtctg ccagtgccgc gtcaacaagt gccgggcagg
      601 cctcagccag tgccaccgcc gccggaaaat cggcagaaag cgccgcatca tccgcttcaa
      661 cagccacaac gaaggctggc gaagccactg aacaggccag cgcagcagcg agttctgctt
      721 ccgcagcgaa gacatccgaa acgaacgcga aagcgtcgga aaccagcgca gaatcctcaa
      781 aaacggctgc cgcatcgtca gccagttcgg cggcgtcatc ggcatcatcg gcgtctgctt
      841 caaaagatga ggcgaccaga caggcgtcag cagcgaagag cagcgccacg acggcatcca
      901 cgaaggcgac agaggcagct ggcagtgcga cggcggcagc tcagagcaaa agtacggcgg
      961 aatccgcggc aacgcgcgcc gagacagcag ctaaacgggc agaggatatt gcatccgccg
     1021 tggcgcttga ggatgcgagc acgacgaaaa aggggatagt acagctcagc agtgcgacta
     1081 acagcacttc cgagtcactg gcggcaacgc caaaagccgt taaggccgcg tatgacctgg
     1141 ctaacgggaa atacaccgca caggatgcaa cgacagcaca gaaagggatc gttcagctca
     1201 gcagcgcgac caacagcaca tctgaaatgc tggcggcaac gccaaagtcg gtaaaggcag
     1261 cctatgacct tgctaacggg aaatataccg ctcaggacgc tacgacagca caaaaaggaa
     1321 ttgtccagct cagtagtgca accaacagcg catctgaaac gcttgccgag acaccgaaag
     1381 cagtgaaagc agctaatgat aatgcgaatg gtcgggtacc ttctgcccgt aaggtgaatg
     1441 gtaaggcgct ttcagcggat ataacactga cgccgaaaga tattggtacg cttaactcaa
     1501 caacaatgtc attcagcggt ggtgctggtt ggttcaaatt agcaacggta accatgccac
     1561 aggcgagttc tgttgtttca attacgttga ttggtggcgc tggatttaac gtggggtcac
     1621 ctcaacaggc aggtatatct gaacttgttt tacgtgcagg taatggtaat ccgaagggga
     1681 ttactggtgc tttatggcag cgcacattga cagggtttac aaattttgcc tgggt
//
LOCUS       DAZGOB010000318         1729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGOB010000318 DAZGOB010000000
VERSION     DAZGOB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.320"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1285
                     /gene="rhsB"
                     /locus_tag="ACHLIF_005288"
     CDS             <1..1285
                     /gene="rhsB"
                     /locus_tag="ACHLIF_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO9609084.1"
                     /translation="EPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYG
                     WDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADTLQQSGGEDG
                     GSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIH
                     LYHCDHRGLPLALISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGL
                     YYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTN
                     GLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPN
                     RGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSF
                     YLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            1257..1640
                     /gene="yhhH"
                     /locus_tag="ACHLIF_005289"
     CDS             1257..1640
                     /gene="yhhH"
                     /locus_tag="ACHLIF_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO9609085.1"
                     /translation="MSAEFMVICKKLLFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
BASE COUNT          529 a          349 c          462 g          389 t
ORIGIN      
        1 agagccgctg gtcgaaagtc gctatcttta cgacccgctg ggccgcaggg tggcaaaacg
       61 ggtatggcga cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga aaccggaagt
      121 gacctggtac ggctgggacg gcgaccggct gaccacgata cagaacgaca gaacccgcat
      181 ccagacgatt tatcagccgg ggagcttcac gccactcatc agagttgaaa ccgccaccgg
      241 tgagctggcg aaaacgcagc gccgcagcct ggcggatacc cttcagcagt ccggcggcga
      301 agacggtggc agtgtggtgt tcccgccggt gctggtgcag atgctcgacc ggctggaaag
      361 tgaaatcctg gctgaccggg tgagtgagga aagccgccgc tggctggcat cgtgcggcct
      421 gactgtggag cagatgcaaa accagatgga cccggtgtac acgccggcgc gaaaaatcca
      481 cctgtaccac tgcgaccatc gcggcctgcc gctggccctt atcagtaagg aaggggcaac
      541 agaatggtgc gcagaatacg atgagtgggg caacctgctg aatgaagaga acccgcatca
      601 gctgcagcag cttatccgcc tgccggggca gcagtatgat gaggagtccg gcctgtatta
      661 caaccgccac cgctattatg acccgctgca ggggcgatat atcactcagg atccgattgg
      721 actgaagggg ggatggaacc tgtatggata tcaattgaat ccgatatcag acatcgaccc
      781 cctgggttta tctatgtggg aggatgcaaa atcgggggca tgtactaatg gtctttgcgg
      841 cacactatcc gctatgatag gtccagataa atttgattct atagatagca ccgcatatga
      901 cgccttaaat aaaataaata gccaatctat ttgcgaagat aaagagttcg ctggtttaat
      961 atgtaaggat aatagtggca gatatttctc aacagcacct aaccgaggag aaagaaaagg
     1021 atcatatcca ttcaatagcc cttgccctaa tggtactgag aaagtatcag cttatcatac
     1081 tcatggtgca gatagtcatg gagaatattg ggacgaaata ttttcaggta aagatgagaa
     1141 aatagttaaa agtaaagata acaatatcaa gtcattttat ttaggtacgc ccagtggtaa
     1201 ttttaaagca atagataacc acgggaagga aataacaaac agaaaaggat tacctaatgt
     1261 ctgcagagtt catggtaata tgtaaaaaac tattgtttag gaactgtgtc attgtatctt
     1321 tgtttgtttt tacatacaac acatgggcgc agtgtaataa taatattaaa ataatgcgca
     1381 agtatgaaag tgaaggtaaa tataccgtta gaaatttggt taaaaataaa gctatagcat
     1441 tggaattagc tgagatatat gttaagaatc gttatggaca ggatgccgca gaagaagaaa
     1501 aaccatacga aatcactgag ttaacaacaa gttgggttgt tgaaggtacc attcactcag
     1561 accaaattgc tggtggggtt tttattatag aaataggcaa aaatgatggg agaattctga
     1621 attttggcca cggaaaataa gtatgtaagg ataatcaaac atcgtgtgcg ctgatggcag
     1681 agtaggtgga ggactccaga cagtcaaacg atagaaaaag atagccttt
//
LOCUS       DAZGOB010000319         1728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGOB010000319 DAZGOB010000000
VERSION     DAZGOB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.321"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            334..1311
                     /locus_tag="ACHLIF_005290"
                     /pseudo
     CDS             334..1311
                     /locus_tag="ACHLIF_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          513 a          391 c          400 g          424 t
ORIGIN      
        1 tcattatatg agaacaccaa taagaataat aatatttgat cacaactgaa gatgcttttt
       61 acaatgaaga attattgtta aaaaacgctt tgttgaatca accgaactgt tgctgagttg
      121 caggatcaca tcacgtattt tcctgaaaac gcaggctgct tagtagcaaa gcagaagttc
      181 aaaatcacca actaaatagc tgcgccgaat agtagatcaa tgaaagggaa ctcagcccgg
      241 attgtgcgat ctgatcaatc gccaaaaatc cccccctaac cggactgagc gatgccgatc
      301 atagcaccaa ttccccgcga cgaatgacgc ctgatgcaaa aagcaatcta taaaactcac
      361 gataaaaatt atgcccgcag gctgaccgcc atgctgatgc tgcaccggag cgctcgcgtc
      421 agtgacgttg ccagaacgct ctgctgcgcc cgttcctccg tcaggcgctg gattaactgg
      481 ttcacgctgt cgagtgttga aggtctgaaa tcattacccg caggacgttc ccgccgctgg
      541 ccgtttgagc atatctgcac attgtttcgt gaactgataa aacactctcc cggatatttt
      601 ggctatcagc gttcacgctg gagtacagaa ctgctggcaa taaaaatcaa cgagataacc
      661 gagtgccagt tacatgccgg aaccgttcgc cgctggttgc cattagtcgg gcttgtgtgg
      721 cgcagggctg cgccaaccct gcgtacccgt aacccgcata aagcagcaat tcacaaagca
      781 ctggacgaat gtagtgcgga gcatccggtc ttttatgaag atgaagtgta tatctatctt
      841 aatcccaaaa ccggtgcgga ctggcaactt cgcagacagc aaaaacatgt ggtaacaccg
      901 gggcagaatg aaaaatacta tctggctggt gctttacata gtggaacggg caaagtcagc
      961 tatgtgggtg gcaacagcaa aagctcagcc ttatttatca gtctgttgaa acatctgaaa
     1021 ttgacatacc gacgggataa acctatcacg ctcatagtgg acaactacat catccataaa
     1081 agtcgggaaa cacagcgctg gctgaaagaa aatccgaagt tcagagtgat ttatcagcca
     1141 gtttactcgc cgtgggtgaa tcatgttgag cggttatagc aggcgcttca cgatacgata
     1201 acgcgcaatc atcagtgccg ttcaatgtgg taactgctta aaaaggttca ttattttatg
     1261 gaaaccgtga gcccattccc cggtggtaaa tatgggctgt taaaagtgta gcggtattcg
     1321 gcgcagctat ttaggatggt caggtcgcag aagttctgtg ccactacagt gcgtgtacta
     1381 aacaaaatga cgagggcacg aatgtcataa tacgtgcgta ttacctgcag tagctaccaa
     1441 ctgagggggc ttatcccaaa cctgatttat tcaacaaagc tggactggac ttatccttac
     1501 tctgaattat tttccagaca tatgcatcat gtactggctc gcttggttca ggaaatacag
     1561 gagcaaatct acagctatta gatttattcc tatttgtatg caaagtcgca ttgcataatg
     1621 cggagctatg ttacagatac gcctgacgtg cgtagtaaaa ttataaagca acatatatat
     1681 acaaggagaa accgagaaaa ataaatgagt cgtatgttgg gtagcatc
//
LOCUS       DAZGOB010000320         1723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGOB010000320 DAZGOB010000000
VERSION     DAZGOB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.322"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            171..842
                     /gene="queE"
                     /locus_tag="ACHLIF_005291"
     CDS             171..842
                     /gene="queE"
                     /locus_tag="ACHLIF_005291"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO9609086.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          438 a          396 c          420 g          469 t
ORIGIN      
        1 ttaaatccat tttctttatt ttcttctgac ataatacgtt actcccttta attattaacg
       61 atatgtttcg catttttctt taaatctgcc tgtaacagaa tgttaataag acatgattgt
      121 tgtggcagga tctgcaataa ttaacaatta tccacttcac agagaatgct atgcagtacc
      181 cgattaacga gatgttccag accctgcaag gtgagggtta ctttaccggc gtccccgcca
      241 tttttattcg tttacaggga tgcccggttg gctgtgcctg gtgcgacacc aaacacacct
      301 gggaaaagct tgaggatcgg gaagtctccc ttttcagcat tctggcgaag accaaagaga
      361 gtgataagtg gggggcggcg agcagtgaag atttgctggc tgtcattggt cggcagggat
      421 ataccgcgcg gcatgtggtg attacgggtg gtgagccttg cattcatgat ttgctgccac
      481 tgactgacct gctcgaaaag aacggtttta gctgccagat cgaaaccagt ggaactcatg
      541 aagtacgctg cacaccgaat acctgggtta ccgtatcgcc aaagctgaac atgcgcggcg
      601 gctatgacgt gttgtcacag gcactggagc gagccaacga aatcaagcat ccagtggggc
      661 gcgtacgtga tattgaagca ctggatgaac tactggcaac gctgaccgat gataaaccgc
      721 gagtcattgc actgcagccg attagccaaa aggatgatgc cacacgtttg tgcattgaaa
      781 cctgcattgc gcgtaattgg cgtttgtcga tgcaaacaca taaatatcta aatattgcct
      841 gattaaacat ttataagcgt tataaatggg tggaacctgt aaagacttct actcatttat
      901 attgtttgtc gcctctgaaa actcctccat tttacccatc cagggctaat cattagcatt
      961 ctctacaaat tctgtggcat taatttttcg ctggagtgaa aattattgcg gtaaagtttg
     1021 gtagatttta gtttgtatag agttatttta aatatttacc tttttaatca atggattaag
     1081 tgctctttaa cataatggat gtgttgtttg tgtgatacta taaagttggt agattgtgac
     1141 tggcttaaaa aatcattaat taataatagg ttatgtttag agtgttcccc gcgccagcgg
     1201 ggataaaccg gatatttggg cgtaataccg cccggatagt gtgtgttccc cgcgccagcg
     1261 gggataaacc gtggtacgga acatgctgtc tatatttcga ccagtgttcc ccgcgccagc
     1321 ggggataaac cgccagtgga gccgccgcga atttacacac gattgtgttc cccgcgccag
     1381 cggggataaa ccgtaaggcc gtcgccggat cagcctggct atgccgagct ccccgcgcca
     1441 gcggggataa accgttcttg cgggtgttgc aaatattctt cacgtagagc tccccgcgcc
     1501 agcggggata aaccggagcc tgacgagact actgaggccg ttctgtcgag ttccccgcgc
     1561 cagcggggat aaaccggacg ccgccgccgc gaagccgttt ccgatgttga gctccccgcg
     1621 ccagcgggga taaaccacca tataacccgt tatctctttc tcaagttttt atattagcag
     1681 tacttgtaat aagcaacata tccacgtaac acctcatgtt caa
//
LOCUS       DAZGOB010000321         1701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGOB010000321 DAZGOB010000000
VERSION     DAZGOB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.323"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..67)
                     /locus_tag="ACHLIF_005292"
     CDS             complement(<1..67)
                     /locus_tag="ACHLIF_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609087.1"
                     /translation="MKHTDIRAAVLDALEQHEHGAT"
     gene            complement(64..639)
                     /locus_tag="ACHLIF_005293"
     CDS             complement(64..639)
                     /locus_tag="ACHLIF_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609088.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPSASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELGYALKQQLRIVIKR"
     gene            complement(654..1007)
                     /locus_tag="ACHLIF_005294"
     CDS             complement(654..1007)
                     /locus_tag="ACHLIF_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9609089.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHLIF_005295"
     CDS             complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHLIF_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9609090.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(1426..>1701)
                     /locus_tag="ACHLIF_005296"
     CDS             complement(1426..>1701)
                     /locus_tag="ACHLIF_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9609091.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          389 a          530 c          375 g          407 t
ORIGIN      
        1 gcgtcgcccc gtgttcatgc tgctcgagtg catccagcac tgcagcacgg atatcagtat
       61 gtttcatcgc tttatcacaa tccttagttg ttgtttcaac gcatagccca gttcttttgg
      121 cagacgttct cgccggatac ggttaacgtt ctcatcaaaa gcctgcttca gtggggccgc
      181 catcggaatt ttaaccacct gaatgggaag acgattacgc tttttccttc ctttatcgtc
      241 attgcccttc gcataccggg cttctggcaa acgttgcata acatgccagc gcccattatt
      301 taatcgctgg atgaatgccc gctgataacg atgctgaccg gctttaagta tgctgttcgg
      361 acgatgacca agcatcctga tccccagctt aatagcaggg agatcaccgc ggttaacgat
      421 aattctggca ttcggatttc tgaccgtcgc ccgtttcagt ctggaccgtt cctttaccag
      481 tttccggctc accctggtct cccgggcaac ctgtgatgaa gactgattaa tcgccgttgt
      541 agccacgcgg ttaatagcca ttgctgaagc cgacggaatg gcgtttttac gaacccggct
      601 cagattttca atcgcctgat caagcccttt tatcgccata attcaccctg cgtttatcgt
      661 cgccggttaa ctgcgggtgg ttgcccacgg ttcagccaga gataacaact gcccccgtca
      721 tccggagaaa cacgatccac ccagaacgtc tcaccattaa tggtcaacgt gtcaccacgc
      781 cgcacggcac gaaccgtatc cgtccgcaca aataatgacg ggctgcttcc ttcaatacgg
      841 atcccactac tggcaaaccc cagcgactcc ggatcgtcaa aaaccccctg aacttcgccg
      901 ccacgctgtg caccggaggt gaactgcgca ctgatgccca tcacttcaac aatcgtactg
      961 tccaccccgg caagggcggc atcaaaggca ttctgaaaat cacgcataaa cagccattcc
     1021 accatcaacg tgtgtttttg catctgagga cataatcaga atcacccgac caacatccgc
     1081 aagctcaacg gattcccccg tttcaccatc aacgccacag agatggaggc aggtcagaac
     1141 tctgatgcgc gttaacgcgc cggatgtttc ctcacgaaca tcatgagccg cgttttccct
     1201 ctcccggata tccatattca taacctgtac atcatcgccg gatgactgca tttcctcttc
     1261 ccattctgcc acccgctgcg ctatctctgc ggcactcccg gatatatccg gctcacgccc
     1321 cagaatcagg gccagttcat caagccgttt cagattttgc tctttcgttg ccatatcatc
     1381 cccctgtgaa aaaagacacg ggggcatttc gcccccgctc acggattatt tcacctgtac
     1441 caccacaaac tcatccgggt ccggcaacac catcagcggc gcggactgcg tcatggtaaa
     1501 ttcacgggcg ggatccccta ccgtcagcca gtgtttcgga taacgggaag aggccaccac
     1561 aatggccaga tcgccgtaat accctttgaa ggacaccacc gcgcccaggt ctttcactgc
     1621 cgtttcgagt tgtgaatttg agccgcgacg ggtatccagt ttttcgcgga acaatttaaa
     1681 gccattcagc agacgccaga c
//
LOCUS       DAZGOB010000322         1684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGOB010000322 DAZGOB010000000
VERSION     DAZGOB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.324"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..102
                     /locus_tag="ACHLIF_005297"
     CDS             <1..102
                     /locus_tag="ACHLIF_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO9609092.1"
                     /translation="IGVLGSLLFGLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(358..630)
                     /locus_tag="ACHLIF_005298"
     CDS             complement(358..630)
                     /locus_tag="ACHLIF_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609093.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            790..1323
                     /locus_tag="ACHLIF_005299"
     CDS             790..1323
                     /locus_tag="ACHLIF_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9609094.1"
                     /translation="MHEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1544..1657
                     /locus_tag="ACHLIF_005300"
     CDS             1544..1657
                     /locus_tag="ACHLIF_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609095.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
BASE COUNT          446 a          320 c          455 g          463 t
ORIGIN      
        1 ataggtgtgc tgggaagtct gctgtttggc ctgctgacgt atctgactaa cctgtatttc
       61 aaaatcagag aggaccgtcg taaggtggcg cggggagagt agtcgatgaa taaacaatac
      121 gaactggttg taaaatgaat atttctaact gaaaaaacgt tccatgaggt aagaaaaggt
      181 cacaggcaat caataacagg acgtgatgaa agacccttgc atttgtgcgc tttctcttta
      241 gatagcagca gatactgaaa atctgagttg tcggggagtc agggatacag ctgtgcaaga
      301 gttggtcatt gtgattccat tgaaatcctg tatgccatga agggcaggat tttatggcta
      361 cctgagcttt ggtgatagta agttgaaaat tcgcattttt tgctgacatg cgtaacgaga
      421 atcccataag cagggaggac ttaattcttc attaacccat gcgttgatat tatgtttcag
      481 ccgttgaagc atcagcggtg ttaatgttgt ggtaataata tccagcgttt tatgtgagat
      541 cttaccgtaa gggtctgcaa gaatgctgct tgttgcttcg ttattatctg ccatcagaag
      601 aagtaactct gatttaacgt tttctgtcat tagttgtaaa aatcttctgc gcaaactttc
      661 tttactgttc atttatatgg cttcatttgt tgtaatctgc tgcgtctcaa gggatatgtt
      721 tatgagagcg accatgagtg ttggattata tacctaacat atcaagggat tagaaatcga
      781 taaatcccca tgcacgaaaa aataaaatac ggcctgtcgg ctgccgttct ggcgctgatt
      841 ggtgcaggtg cttctgcgcc tgaaatcctc gaccagtttc tggatgaaaa ggaaggtaac
      901 cacaccacgg cataccgtga tggtgcgggg atctggacca tctgccgtgg tgccattctg
      961 gtggatggta agcctgttat tcctggcatg aagctgtcaa aggaaaaatg cgaccgggtt
     1021 aatgccatcg aacgtgacaa ggcgctggca tgggtggaga aaaacatccg ggtgccgctg
     1081 accgaacccc agaaagcggg gatcgcgtca ttctgtccgt acaacattgg ccccggtaag
     1141 tgcttcccgt cgacgtttta taaacgaatt aatgcaggcg atcgaaaagg tgcctgtgag
     1201 gcgattcgct ggtggattaa ggacggtggc agagactgcc gtatccgttc aaataattgc
     1261 tacggtcagg tctcacggcg tgaccaggag agcgcgctgg cgtgctggga catcgacaga
     1321 tagcagaata ttttcctgaa aaatgacgtt ggccaacgcg ggtggataac acgaaatcct
     1381 gaaaactggt aaaacctaag tgaataaaag taaaaacccc gtttgttggc agcaagcggg
     1441 gttttgtgtt ttctgacctt gagcaaggca agggagaaat tatgggtagg gaggtacttt
     1501 ccctgtgagg aagtataaaa gattctttct gaggttgtcc attatgaaag gcattgaagt
     1561 ggagacgcca gccagtctgg atttaacaag agcggcagct tttgccattc gtattgtggc
     1621 cattgctgtt ctggtctggg caatccgttg gtggtgatat gaaccgtgtt ctgtgcgtgg
     1681 ttat
//
LOCUS       DAZGOB010000323         1677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGOB010000323 DAZGOB010000000
VERSION     DAZGOB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.325"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..202
                     /locus_tag="ACHLIF_005301"
     CDS             <1..202
                     /locus_tag="ACHLIF_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609096.1"
                     /translation="ELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFGSI
                     FLWVIIGVAPVAVAKMAWRFVS"
     gene            210..656
                     /locus_tag="ACHLIF_005302"
     CDS             210..656
                     /locus_tag="ACHLIF_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO9609097.1"
                     /translation="MKQIYMLRNEAIRNNAIDAILSLPIDDKSPHEVHIKEPKRSNPQ
                     NRLMWALLQDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            653..1180
                     /locus_tag="ACHLIF_005303"
     CDS             653..1180
                     /locus_tag="ACHLIF_005303"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO9609098.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            1177..1359
                     /locus_tag="ACHLIF_005304"
     CDS             1177..1359
                     /locus_tag="ACHLIF_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO9609099.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRYRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
BASE COUNT          452 a          349 c          462 g          414 t
ORIGIN      
        1 tgagttgatt ttttctgcat tgaggattct cggtgctatg tggatggtgg cgacgttcat
       61 tgtggttgcc agcagttttg tccggctggt aggcgaaggt aaagacctgg ttggtgtgct
      121 tttcggtagc attttcctgt gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat
      181 ggcgtggcgt tttgtgagtt gaggtgacaa tgaagcaaat atacatgctt cgcaacgaag
      241 caatcagaaa caacgccata gacgcaatac tctcacttcc gatcgacgac aagtcacctc
      301 acgaagtcca cattaaagaa ccaaagcgga gcaatcctca aaaccgcctt atgtgggcgt
      361 tattgcagga cgtatcacgt caggtgcttt ggcatggaca gagacttgcg ccggaggact
      421 ggaaagacct gttcactgcc ctgtggctta agaccaaaaa actggagcaa cgaagtgtgc
      481 ctggtatcga tggtggcgtt gtcatgcttg gcgtgcgtac cagcaaaatg cgaaaggcca
      541 gcatgactga gcttatcgaa atcatgtttt ggttcggctc agagcgcaac gtgcggtgga
      601 gtgatgactc ccggcgagag tatgaatggt cacaacgaaa aggtagggct gcatgactat
      661 caaatcaaat acaccagcac acgacaagga ctgctggcaa acgccgcttt ggctttttga
      721 tgcgctggat attgagtttg gattctggct ggattcagct gcgagcgaca aaaatgctct
      781 gtgcgctcac tggctaactg aggccgacga cgcactcaat tctgagtgga taagccacgg
      841 tgcaatctgg aataacccac cgtacagcaa tatcaggccg tgggtggaaa aagccgctga
      901 gcagtgcata caacagcgac agacggtagt gatgcttgtg ccagaggata tgtctgtcgg
      961 atggttcagc aaggctctgg agagtgttga cgaagttcgc attatcactg atggacggat
     1021 taattttatc gaaccatcga cagggctgga gaagaaggga aacagtaaag gctccatgct
     1081 gctgatttgg cgaccgttca tcagtcctcg acggatgttt actaccgtat ccaaagcggc
     1141 attgatggcg atcgggcagg gcgtcaggag ggcggcatga ggcgacagcg acgaagtttc
     1201 accgacatca tctgcgaaaa ctgcaaatac cttccaacga aacgctacag aaataaacgc
     1261 aagccaatcc caaaagaatc tgacgtaaaa accttcaatt acacggctca cctgtgggat
     1321 atccggtggc taagacatcg tgcgaggaaa acaaggtaat tgactaaaat cgaagttacg
     1381 aacaagaaag cgtcgagcgg gcttcagtgt acactgagtg gattctatct aggcttagtg
     1441 catacagaag attgctggta aaggacatgc caggcaaaac gatgaggact gatatttatg
     1501 aaaacatctg attttttact gttcttgcat gcggtacagg aggggctttg accgggcatt
     1561 ttatcgtgaa tattttcact tggtatttct ttggttttag agattacttc actcgatggg
     1621 ttttaaatag ttttcgtcgg tttatcgggt gcaagcctga tatgagaatt tataaag
//
LOCUS       DAZGOB010000324         1667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGOB010000324 DAZGOB010000000
VERSION     DAZGOB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.326"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..261
                     /locus_tag="ACHLIF_005305"
     CDS             <1..261
                     /locus_tag="ACHLIF_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9609100.1"
                     /translation="NEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTL
                     PLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
     gene            274..648
                     /locus_tag="ACHLIF_005306"
     CDS             274..648
                     /locus_tag="ACHLIF_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9609101.1"
                     /translation="MRIEFVLLYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGLVKK"
     gene            645..1466
                     /locus_tag="ACHLIF_005307"
     CDS             645..1466
                     /locus_tag="ACHLIF_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9609102.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
BASE COUNT          430 a          339 c          475 g          423 t
ORIGIN      
        1 aatgagaaat acacacgctg ggtaaagaca cagccgtgtg cgtgttgtgg taagccagcg
       61 gacgatcctc atcatctgat tggtcatggt cagggtggaa tgggaacaaa atcccacgat
      121 attttcacgc taccgctgtg tcgggagcat cacaacgagc ttcatgcgga tccgctggcg
      181 ttcgaagaaa agcatggttc ccaggttgat ttaatttttc gttttcttga tcacgccttt
      241 gcaaccggcg tgctcgggta aaagaggtta ctgatgcgta tagagtttgt tttgctttac
      301 ccgccgacgg tgaacaccta ctggcgacgt cgtggcagca catattttgt atcaaaagcc
      361 ggtgagcgtt atcgccgggc tgtggcgctt attgttcgcc agcagcggct gaaattaagc
      421 ctgtccggaa ggctggcgat aaagattatt gccgagccac cggataagcg ccgccgtgac
      481 ctggacaata ttctgaaagc gccgctggat gcgctgacgc atgcgggagt gttaatggac
      541 gatgagcagt ttgatgaaat caatatcgtt cgtggtcagc cagtatctgg tggacgtctg
      601 ggggtgaaga tttaccccat aatgcttgaa gggctggtca aaaaatgaaa ctggaagatt
      661 taccgaaata ctactcccca aaatcccccg gcctgactga tgcatcggcc tcaacgtcga
      721 aagatacgct gagtatcact gatgtgatgg ccgcgcaggg catgacacag aattgggctg
      781 agatggggtt ttctgcgttc cttgggaaaa tgggcattag tatgaatgac agagagcggg
      841 caacagaatt gctgacagaa tatgcactca gtcggtgtga tcgcgtggcg gcgttaagaa
      901 aactcccggc agaaataaaa ccggcagtga tgcgtattat ggcttcgtat gcgtttgaag
      961 attatgcccg tagcgcggcg agcaaaaaac agtgcccctg ttgtcacgga aaaaaattta
     1021 ttgaaagcga ggtttttaca aacaagatcc agtatccgga tggtaagccg ccagtgtggg
     1081 caaagtgcac aaaaggcgtg tatccgtctt actgggagga atggaaaaaa gtcagggagg
     1141 tggtaaaagt tgcctgtccg gagtgtggag ggaaggggga ggtttccacc gcctgtaaag
     1201 attgtcgtgg gcgcggtgtt gccattcatc gtgaagagtc ggtaaaacgt ggtatgcctg
     1261 ttatcagaga ctgccagcgt tgtggtggtc gtggctatga aagattacct tcaacggagg
     1321 catttaatgc catatgtaat gtaaccgatg ccatatctct tgatacatgg aaaaaaacag
     1381 ttaaacgttt ttacgatacg ctggtggtgc agtttgatat tgaagaagca tgggcagaac
     1441 aacaactgaa aaaggtgacc agatagcttt gttgattttt cccgaatctg tggtaaattt
     1501 gccctaacga tgggcgtttt atgcctgacg ttagaagatt ttttacaccc gtcgccaggc
     1561 gggttttttt atgactgaaa tcacgccagt acagtaaacg cgctggtggt tgtgaatacc
     1621 ggtctttcag cttgctggct ttttcgacaa gagttattgg tgtgtca
//
LOCUS       DAZGOB010000325         1665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGOB010000325 DAZGOB010000000
VERSION     DAZGOB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.327"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..210)
                     /locus_tag="ACHLIF_005308"
                     /pseudo
     CDS             complement(1..210)
                     /locus_tag="ACHLIF_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspG"
     gene            270..967
                     /locus_tag="ACHLIF_005309"
     CDS             join(270..519,519..967)
                     /locus_tag="ACHLIF_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFO9609103.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            complement(991..1209)
                     /locus_tag="ACHLIF_005310"
                     /pseudo
     CDS             complement(991..1209)
                     /locus_tag="ACHLIF_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
     gene            complement(1385..>1665)
                     /locus_tag="ACHLIF_005311"
     CDS             complement(1385..>1665)
                     /locus_tag="ACHLIF_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9609104.1"
                     /translation="GERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISK
                     RYTQRIERHNLNLRQHLARPGRKSLSFSKSVELHDKDIGHYLNIKHYQ"
BASE COUNT          447 a          401 c          387 g          430 t
ORIGIN      
        1 catattacct gtattaagat cgtaatggat tatactcaac tcattcaatt tacatatcaa
       61 ctgcagataa aggctctcta gattatcagg aatatcagct gtatcaatat cactaagggg
      121 aatccctggc acgcgtagca ttcggagata cacatcaccg ccatactgta taacaactga
      181 tgcgtcatcg ccataaaaag tattaaaaag ggcaggtgat gctgccaact tactgattta
      241 gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct
      301 gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc
      361 tctgctctca ctgccgtaaa acatggcaac tgcagttcac ttacaccgct tctcaacccg
      421 gtacgcacca gaaaatcatt gatatggcca tgaatggcgt tggatgccgg gcaactgccc
      481 gcattatggg cgttggcctc aacacgattt tacgtcactt aaaaaactca ggccgcagtc
      541 ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca
      601 gtggggctat gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt atgacagtct
      661 ccggaagacg gttgttgcgc acgtattcgg tgaacgcact atggcgacgc tggggcgtct
      721 tatgagcctg ctgtcaccct ttgacgtggt gatatggatg acggatggct ggccgctgta
      781 tgaatcccgc ctgaagggaa agctgcacgt aatcagcaag cgatatacgc agcgaattga
      841 gcggcataac ctgaatctga ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt
      901 ctcaaaatcg gtggagctgc atgacaaagt catcgggcat tatctgaaca taaaacacta
      961 tcaataagtt ggagtcatta ccaaaagggc agattcttgc ctagccattt ccagaacttc
     1021 attgtgctgg ttaccaacaa gatcatactt tttatataaa gcagacgaat cattcatatc
     1081 ttcaaagatt tctgctgttg aaccttgccc tattaactta ccgagaatag gctccgttac
     1141 tacgccagta tgagaattat tattctcaaa tgggaaaggt gtttgaataa cagtggatat
     1201 tattttcatg acacgccctt gttatgaaat ttaaagatta caagatattt ttacataaaa
     1261 taatattacg cgctaccacc tcagaaacca cgtaagtaca tatgctatta catagataac
     1321 cgccatgatc agggaagccc catacaagca cagtgtagag aggcctttag gtaatgactc
     1381 caacttactg atagtgtttt atgttcagat aatgcccgat gtccttgtca tgcagctcca
     1441 ccgattttga gaacgacagt gacttccgtc ccggccttgc caggtgttgt ctcagattca
     1501 gattatgtcg ctcaatgcgc tgagtgtaac gcttgctgat aacgtgcagc tttcccttca
     1561 ggcgtgattc atacagcggc cagccatccg tcatccatac cacgacctca aaggccgaca
     1621 gcaggctcag aagacgctcc agtgtggcca gagtgcgttc accga
//
LOCUS       DAZGOB010000326         1658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGOB010000326 DAZGOB010000000
VERSION     DAZGOB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.328"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(20..156)
                     /locus_tag="ACHLIF_005312"
     ncRNA           complement(20..156)
                     /ncRNA_class="other"
                     /locus_tag="ACHLIF_005312"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            375..1577
                     /locus_tag="ACHLIF_005313"
     CDS             375..1577
                     /locus_tag="ACHLIF_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9609105.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT          438 a          349 c          443 g          428 t
ORIGIN      
        1 acgagctctt ctttaaatat ggcggtgagg gggggattga ctcgcttcgc tcgccctgcg
       61 ggcagcccgc tcactgcgtt cacggtctgt ccaactggct gttgccagtt gtcgaacccc
      121 ggtcggggct tctcatcccc ccggtgtgtg aaatatacga aaaaaaagcc cgtactttcg
      181 tacgagctct tctttaaata tggcggtgag ggggaccgcc tgaaataaat ctgacttaat
      241 ccaggggtgt tatataattt cattataaac aatgtattac actttttgtt gtccgatatt
      301 atctaatgta ttcttagtga agcgagagac taatggcata ctggatggca taccagagtg
      361 acgggaatta gcgtatggca gtattgacgg atacgaaagc aagacatatc aaacctgatg
      421 acaaaccatt gccccatggg ggaattaccg gactgaccct tcatccttct tcagtaaagg
      481 ggcgggggaa atgggttttt cgttatgtaa gtccggtgac acaaaaaaga cgtaatgctg
      541 gattgggaac ttacccagag gtcagtattg ctgaagctgc acgtactgcc cggataatgc
      601 gagagcaact tgctgcaggt gatgatcctc tggagattaa aaaggctgaa tctgagaaag
      661 tcgctatccc aacatttgcc gatgcagcca ggcgtgtaca tgcagaactg tctcctggat
      721 gggaaaatcc aaagcatgta aggcagtggt tatcgacgct tgagaattac gcgtttcctc
      781 aactgggagc aaaaacgctg gattcgatta cggctgcgga cgtggcagaa acactgcgtc
      841 cagtctggtt aaccttgtca gaaacggcaa gccgggttaa acagcgcatt catgttgtta
      901 tgcagtgggg atgggcgcac ggtttttgtg tagcaaatcc tgttgatgtg gttgaccatt
      961 tgcttcctca gcagacaaga ggacgtgatg aacaccaacc cgcaatgccc tggaggcagt
     1021 taccgctttt tgtggcgacc agtgtgtata ccgatgaacc ttataatgtt acccgcgcac
     1081 tgttattaat ggtgatactt acagcaactc gctcgggcga agccagggga atgcgctggg
     1141 ctgaaattga ttttcataag cgggtatgga ctatacctgc agaaagaatg aaagccagga
     1201 tacagcatcg tgttccttta tcccggcagg ctatttacat tctggaaaat atacgtggcc
     1261 tgcatgatga actggtgttc ccttcaccca gaaagcagca gatcctttcc gatatggtgt
     1321 tgacaagttt tctgcgtaaa aagaaagccg tcagtgacat tccggggcga gttgccacgg
     1381 cacatggttt tcgctcaaca ttcagggact ggtgtagcga acaggggtat tcgcgggatc
     1441 tggcggaaag ggcgctcgct catacgctga aaaataaggt tgaggcggca tatcatcgta
     1501 ctgatctact ggagcagcgt gtaccgatga tgcaggcatg ggcggattat gtgatgtctc
     1561 aaattgtgaa taaataatag tgaaaatgta tattgtacga tcatgatttg atatgtgatc
     1621 gtagcagtag taattaagaa accgctttta tgtcaaat
//
LOCUS       DAZGOB010000327         1656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGOB010000327 DAZGOB010000000
VERSION     DAZGOB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.329"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..102
                     /locus_tag="ACHLIF_005314"
     CDS             <1..102
                     /locus_tag="ACHLIF_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO9609106.1"
                     /translation="IGVLGSLLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            106..354
                     /locus_tag="ACHLIF_005315"
                     /pseudo
     CDS             106..354
                     /locus_tag="ACHLIF_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            1409..>1656
                     /locus_tag="ACHLIF_005316"
     CDS             1409..>1656
                     /locus_tag="ACHLIF_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9609107.1"
                     /translation="MNAKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLTKEKCDQVNAIER"
BASE COUNT          466 a          337 c          431 g          422 t
ORIGIN      
        1 ataggtgtgc tggggagtct gctgtttggc ctgctgacgt atcttactaa cctgtatttc
       61 aaaatcagag aggaccgtcg taaggcggcg cggggagagt agacgatgaa taaacaatac
      121 gaactggttg ttaaagggat aaatatttac ccggataaga ttactgttac tgtggcgctg
      181 gaaactggtg ggtatacgtc actgttgttg ccaaatgtgg tgattgatct tgatcgtgtt
      241 gaaggtgccc cgctggagtt ttacgaagct gaggcgaaaa agaaggcgaa gcagtttttc
      301 atggatattg ctgccgggtt atgtgaaggg aacgaaccgt tgccggaaaa gcgcctctgt
      361 tcagaagagc ggtataccat acaaattaac aatgcatata acacgataca gtcagaaaaa
      421 gatgatatcg aatcacgcat tgaaaaactg gagaacagcg tcgttgaact gaataaaaaa
      481 ctgtcggtgc agatcccttc cgaaggtgaa aaaaaacgcc gtgatgagca gttcgcggcg
      541 ttttacgatt attgccggaa agtgatgagc agaaatctcg cagagtgttt ccgtattcaa
      601 aaaaacagag agcgctctgc aaatagtgag attctggata attttttaaa ggggacgcat
      661 ctcgccagtg attgtttcat tgctcattct tttggtggct ttcatgaggc catcaaacat
      721 gatgttgaga gaagtccagg tatcctctgt tccattcccg taagcatcga aatagacaca
      781 aatacgataa ccggtgaact cgttaccgcc ggagtcggta tattccgtca tgaggggaac
      841 gatgccaaag gttcctttac aaaaatcggg ccgttctttc tgaaggtaga gcgatagttt
      901 gttaagtacc catccgacag ctcgctcatt ttggtttatt ttcgtatcgc gaaacaaaaa
      961 ttcgaagcgg ccagatgacg catgggtgca accaaatacc ggggcgtatt catcaacctt
     1021 tggtgttcct gaaaacatgc agtccgtgct ctcgaatgca gatgaattgc tgttgatcca
     1081 cttaacaaaa gaaaaaaagg tctgaatcgc ttgtctgtct gaatattcaa caaaatcctc
     1141 aaggtgtgtc tcgatcagtt ccgggtgttt acgaaagtca taatattgac cgggacgata
     1201 tttatgagtg gttgttttat cccatggttg tgctctgcgt ccctgtaaac ggaggtctgc
     1261 cgctggatag ataagcataa gtaatctcct ggtggaatgt aatccacgat catccgggga
     1321 aattaagaac ccgccagtgc ccaccactgg tggggtgaag gcttaacata tccagggatt
     1381 cggaaccgat aaatcctgat aaatatccat gaacgcaaaa atcaaatacg gcctgtcggc
     1441 tgccgttctg gcgctgattg gtgcaggggc gtctgcgcct gaaatcctcg accagtttct
     1501 ggatgaaaag gaaggtaacc acaccacggc ataccgtgat ggtgcgggta tctggaccat
     1561 ctgccgtgga gccacccggg tggatggtaa gcctgttatt cctggcatga aactgacgaa
     1621 ggaaaaatgc gaccaggtta acgctattga acgtga
//
LOCUS       DAZGOB010000328         1640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGOB010000328 DAZGOB010000000
VERSION     DAZGOB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.330"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..65
                     /locus_tag="ACHLIF_005317"
     CDS             <1..65
                     /locus_tag="ACHLIF_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO9609108.1"
                     /translation="CCAELMSDPNSSMYEEEDDE"
     gene            58..780
                     /locus_tag="ACHLIF_005318"
     CDS             58..780
                     /locus_tag="ACHLIF_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO9609109.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEETAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            780..1070
                     /locus_tag="ACHLIF_005319"
     CDS             780..1070
                     /locus_tag="ACHLIF_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO9609110.1"
                     /translation="MANLRKESRGRECQVRIYGICNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            1067..1429
                     /locus_tag="ACHLIF_005320"
     CDS             1067..1429
                     /locus_tag="ACHLIF_005320"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9609111.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            1426..1614
                     /locus_tag="ACHLIF_005321"
     CDS             1426..1614
                     /locus_tag="ACHLIF_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO9609112.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
BASE COUNT          495 a          377 c          422 g          346 t
ORIGIN      
        1 actgctgcgc agaactgatg agcgatccga atagctcaat gtacgaggaa gaagacgatg
       61 aatgagttaa taaatggcaa tgccatcaaa atgacaagca ttgaaatagc tgagttggtg
      121 ggtaagcgtc atgacaatgt gaaacgtacc atcgaaacgc tggctaaaaa tggtgttatc
      181 cggcttcctc aaattgaggt ttccgaaaga atcaataact tagggttcaa tgttcagtac
      241 gagcattacg tcttcgaggg cgaacaaggt aagcgagaca gtattgtcgt tgttgcccag
      301 ttgtcgccag agttcaccgc tcgtcttgtt gaccgttggc gagagcttga agaaactgcg
      361 gttaatatcc ccaaaacgtt accggaagcg ttgcgccttg ctgctgatct tgctgagcag
      421 aaaatgcaac tggaaaacca gctcgcaatt gccgcaccta aagttgagtt tgccgatcgc
      481 gttggcgagg ccagcggaat tttgattgga aactttgcaa aggttgttgg tattggtcca
      541 aacaaactgt ttgcgtggat gcgcgatcac aaaatcctta ttgcttcagg ttcccggcgc
      601 aatgtgccaa tgcaggaata tatggatcgc ggctatttca cagtgaaaga aacagcggtc
      661 aacacaaatc acggaataca gatatcgttc accacaaaaa tcaccgggcg tggtcaacag
      721 tggctgacca gaaagctgct cgataacgga atgctgaaag taacagggga ggctgcttaa
      781 tggctaacct acgcaaagaa tctcgcggca gagaatgcca ggtacgtatt tacggcatat
      841 gcaatggcaa tcctgaaact acagttctgg cacattaccg gatggctgga atttgcggaa
      901 cgggaatgaa gcctgacgac ctgatcggcg catgggcttg tagcgcgtgt cacgatgaaa
      961 tcgaccgacg cacccataac ctcgacaaca aagacgccag actttaccac ctcgaaggcg
     1021 tgatcaggac gcaggcgata ctgctgaagg aggggaagat taagtcatga acgaatatca
     1081 gtttgtgctt ccatacccgc cgtcggtgaa tacctactgg cgaagacggg gaagccaata
     1141 ctacatcagc gataaaggcc agaaataccg aaaagacgtt cagcaaatca tccaccaact
     1201 caagttagat attttcacca aatcacgact ccgcatcaaa gtaatcgcag acgttccaga
     1261 ctcccgccgc cgcgacctag ataacatcct gaagggttta ctcgactccc ttatccatgc
     1321 cggatttgcg gaagacgacg agcaattcga tgacattcgc gtaattcgtg gtgtgaaagt
     1381 accaggcgga cggcttggaa taaaaatcac cgaactggag aacgcatgaa cgccacaatt
     1441 caaacgatac cagagcttct tatccagaca cgaggcaatc agaccgaagt ggcgaggatg
     1501 ctttcctgcg caagaggaac agtgctcaag tacaaccgag acagcaaagg cgagcgtcat
     1561 gtaatagtta acggcgtcct gatggtcaaa cagggcaaga ggggaagacg atgagactcg
     1621 aaagcgtagc taaatttcat
//
LOCUS       DAZGOB010000329         1636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGOB010000329 DAZGOB010000000
VERSION     DAZGOB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.331"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..677)
                     /locus_tag="ACHLIF_005322"
     CDS             complement(<1..677)
                     /locus_tag="ACHLIF_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO9609113.1"
                     /translation="MERLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRRVKFS"
     gene            685..1110
                     /gene="tnpA"
                     /locus_tag="ACHLIF_005323"
                     /pseudo
     CDS             685..1110
                     /gene="tnpA"
                     /locus_tag="ACHLIF_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1174..1467)
                     /locus_tag="ACHLIF_005324"
                     /pseudo
     CDS             complement(1174..1467)
                     /locus_tag="ACHLIF_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT          431 a          389 c          361 g          455 t
ORIGIN      
        1 ttgctgaatt tgaccctgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
       61 ttctgaaaac tgtttacagg ctcaaagact gtgccatctg acagcgtggc gagtttagcc
      121 acgccagcat ccagcccgac cattgatgct gaagggtgaa ccggagtgga tacttcactt
      181 tctgtctgaa tactgatgta ccacttaccg caggactggc tgacagtgac atttttcaca
      241 actcccgtga cctgacggct attccggtag cgcatccagc caagtttcgg cagaaaaata
      301 cggctgtttt cctgatcgag cttaacgccc tgcgggtagc ggaatgcatc attctgtccc
      361 cgctttttga atcggggaaa agcagcccga ttctggaaga agtttttgta agcccgctca
      421 aggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca ttgcgtttca
      481 gtggcgtttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
      541 ccggcctcat gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg
      601 cctgcgaagc gcctcatttc acgctcctgt tgaccaccgg gtcttaactg gaatttaaat
      661 gcctgtagtc gttccacagg tcaaattata ttttggtctg tgaaaaaaga aaccgatatc
      721 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccaaatac
      781 cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
      841 tgtgctgatt ttgaagctga actggttgaa atggatggcg aaccagatca cgtccatttg
      901 ttaattagct atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta
      961 tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
     1021 gttttgtgga gtcctggcta ttttgccaat agctgcggag gtgcgccaat atccgtcatc
     1081 cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc
     1141 cgccctgaag gacggggttt tacggcgcac cagataaacc aggccaagcg tatgctggtt
     1201 gatcaaataa tatcccccaa gcgcagcaat attaatattc acctgcaatg ggtcgatacc
     1261 tggcttaaaa atattgtatt tcttcccttc atccaataag gattccatta actgcaagtg
     1321 cgcatggtta atttctaaga gacgctgtga ttttgcataa tgcacacctt tactttgatt
     1381 ttcactatgc acaattttta aaaaccaagg gttagcaata taataacccc atgtaaaatc
     1441 aataattttc tcaatggctt tttgtggctg gttaaccaga tgattaacaa acgcaccgat
     1501 accaacagcg tagaataatt acataccgca caacaagaat gtatggtgga aggcgtggaa
     1561 gtcattttct ccccgggggc atcgttatat ccataatgag cgatttttga tattaatatc
     1621 tttcaccatc cgctat
//
LOCUS       DAZGOB010000330         1603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGOB010000330 DAZGOB010000000
VERSION     DAZGOB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.332"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            730..1203
                     /locus_tag="ACHLIF_005325"
     CDS             730..1203
                     /locus_tag="ACHLIF_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609114.1"
                     /translation="MDIDTLGGQELRDKIFAGLKIYEGKPFIERFGLFMGKAQLLEFG
                     LKKILVSFPGYNLDEEKLERLTLGQTRVELKKLGLRTDYNAYLKSFKDQRNTMAHEFL
                     ANFAVTQQLLDGAALIRTFERELDHACYAVEQLIILFDFINGAGDVTAWLEPTAP"
BASE COUNT          443 a          353 c          345 g          462 t
ORIGIN      
        1 ttttggcaac tatttttgat tgtttttcaa gaataattcg aaagctatta attaatggct
       61 ggaactaata ggttccagtt ttcaactaat ccagtgacct gctccccgct gatcaactca
      121 gactgttgtt agcaacttac gctcctggta cggagcgggc attgattacc cgctccgttc
      181 acgttccgcc tggaagttct gcaatttatg tctatcggct attgaaaacc ttccggtcct
      241 tcccaatatc gaaactggca cctttgccag ataacaaaga gatacgggaa catgatgtac
      301 gtctacagaa gacctccaaa tcagctcctt tccatttctc gtttgaagca gtatttgttg
      361 catgaggtgg aatcaaatca acacatcatg agacagtggg aatctgccag aacggcactt
      421 cgaaacatcc gtaacaaagg gcatgatcca gacaccgcct cctgggtttc acttaaaaat
      481 gatccccgag tcttcacgaa tggcgcagat gaaaagcatg ggttcatttt tgttgacgac
      541 gacgatcaat cagtccttgt tgtggcctcc tgggtgatag atgagtacgg ttcccccctc
      601 actacatatt aatccctgta cctggacaga agagttttct gtccacaact accaatagct
      661 ttacataatg tgtgcatcta acttcaataa gttacttcat taccaataat gtctaaaaag
      721 gaaacgcgca tggatattga tacgctgggt ggccaggaac ttcgggataa gatttttgct
      781 gggctcaaaa tatacgaggg taaacccttc atcgaacggt tcggtctgtt tatgggcaag
      841 gcacagttgc tagagtttgg cctaaaaaaa attcttgttt cttttccggg atacaacctt
      901 gatgaagaaa aattagagag gctgacatta ggccagaccc gcgttgagct taaaaagctg
      961 gggctgcgca cggactataa tgcttacctg aagtcattca aagatcagcg caatacgatg
     1021 gctcacgagt tccttgctaa ttttgcggtc acacagcagc ttttagacgg agctgcactt
     1081 attcgcacat ttgagcgtga gttagaccat gcctgttacg cggtggagca gttgatcatc
     1141 cttttcgact tcatcaacgg tgccggtgac gtaaccgcct ggcttgagcc tactgccccc
     1201 tgatacttcg tatgctttta tctctaggct ggacagaata gtaattctgt ccaggtatgc
     1261 agaatggctg tctatatatc ttcattagtt cacagtaggt taccggcggc tttgagcgaa
     1321 cagcggaact tcgcccttta ataaatgata ataaattttc attatcactt tagttgaaaa
     1381 tggtgtcgaa gatgaagaga aatgtcatat tagcctacgg agtacttgtg aaataaattc
     1441 atagcattcc ctaacatcta aacttagggc tcactgaacg atggtgaacc ctatgccatt
     1501 gggttaataa ctattgataa ttttgtgaag tgcagggtta attgtcgatg aaatatcaaa
     1561 atcggaattt ctaatcgacc cagtgctcca tccctctttg aaa
//
LOCUS       DAZGOB010000331         1596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGOB010000331 DAZGOB010000000
VERSION     DAZGOB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.333"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..139)
                     /locus_tag="ACHLIF_005326"
     CDS             complement(<1..139)
                     /locus_tag="ACHLIF_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO9609115.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YN"
     gene            complement(155..889)
                     /locus_tag="ACHLIF_005327"
     CDS             complement(155..889)
                     /locus_tag="ACHLIF_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO9609116.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            complement(886..1217)
                     /gene="sctI"
                     /locus_tag="ACHLIF_005328"
                     /pseudo
     CDS             complement(886..1217)
                     /gene="sctI"
                     /locus_tag="ACHLIF_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            complement(1237..1477)
                     /locus_tag="ACHLIF_005329"
                     /pseudo
     CDS             complement(1237..1477)
                     /locus_tag="ACHLIF_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
BASE COUNT          477 a          316 c          270 g          533 t
ORIGIN      
        1 gattgtactg cagaacaatc atttcattgg taatagatct aagcaccgga ttgttaatag
       61 gtgtattatt caagcttact cgttgaggat gaatataact aattggatca tatataatct
      121 ttcttagggc aagattcata acgctctcat atgtctactt aattttcaac ctgactaaat
      181 tagttagaat ggccctatac ttccataaca gccagcaagt cgctacggat attaatgcaa
      241 gtaagataga aaccggcata gccttatcat aagcaaaaac aggttcgcta atttcatatg
      301 ttggtgcttg ctcaataatg tctcttcgtt ttgacaatac aacagaaata ttttcatatt
      361 gtacgcttgc agagctatta acaataaatc tcttgatatc atttattttt atttctgggt
      421 tgatatcttt ttcatatact gcaagtacag aaatatgaat tggtaaagca gttttaccac
      481 tatcgccatt atcaacatcg taactaacat gtactctcga agaaataatg ccatccataa
      541 ttttgagaga ttgctctaac cgctgctcaa tagcagaata tagccttgct ttttccgctc
      601 gtggagatga aacgagtgca tctgcaggga acatctgcga tatttgaata tcaggcttac
      661 ccggtagatt gtagattttt agccaatcca ccgcagaagc aaaatccgtt ggttcgacaa
      721 atattgaaaa tcctgttttg ccttgatcct tcttttcagc attaatatta tgtctttgta
      781 aaacagcaag gacatcatta gcttgctgtt gatcaagatg gttcaataat tcctgctgct
      841 tgcagccgca caacagcagg ataaacaata ataatgatat atatttcatg agcgggtcaa
      901 cgtctcaact gcagcgacaa tctttcttgc taatgttcca ccaatgaaac ctcgacatta
      961 taattaagca catgatcctg caagactaat aatttttcag gagttaaccc ctctttatca
     1021 gaccttaatg cttgtaacgc attttgctgc caattttcag tttgtacagc atagttggca
     1081 aaagcggaga tcattttatc atccgggttg tcaagattga gaacccggtc atttaaatca
     1141 atcactttcg cttgctgtat ttcatgagca gttgattgct cagctctatc aataggtggc
     1201 atattgctta ctaacataat aaaatcctta tctagtttag cggaagtttt gtatgattgc
     1261 tgaatcaaga tctttatacg cttttacaac gttagattgc gcatttcgat ataatgtata
     1321 ctcagctaat gcactttgat attcggcaag aaatttcgtg tcggagggat tggttggcta
     1381 aatcctccaa cgctttttca acttgctggt tcagatcatc aacgccctga tcaaattgat
     1441 tgctgatatc cataatataa ccattccaat ctgccataag tcctcctcaa ttattcctga
     1501 aatgtccagt tgctaccttg agacttctcg aatcggttat ctccgtagct aaatgaacta
     1561 ttatttgtgt cctgattttt caaattcaca ttaaat
//
LOCUS       DAZGOB010000332         1587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGOB010000332 DAZGOB010000000
VERSION     DAZGOB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.334"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(125..334)
                     /locus_tag="ACHLIF_005330"
     CDS             complement(125..334)
                     /locus_tag="ACHLIF_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO9609117.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVSLEDAMLCW
                     GEDEDKVESDPFAALEDALSFAAMS"
     gene            complement(430..804)
                     /locus_tag="ACHLIF_005331"
     CDS             complement(430..804)
                     /locus_tag="ACHLIF_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO9609118.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(819..1535)
                     /locus_tag="ACHLIF_005332"
     CDS             complement(819..1535)
                     /locus_tag="ACHLIF_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO9609119.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRMKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
BASE COUNT          352 a          506 c          368 g          361 t
ORIGIN      
        1 gcacgctgga gctccgactg gaatgactgt gaatttgccg aaattttgat aatcagttca
       61 cgtaacgtcg ccattcacct ttctccgggc aaaaaaacct gccacagcag gttttcatca
      121 ttatttatga cattgctgca aagctcagcg cgtcttccag cgccgcaaac ggatctgact
      181 ccactttatc ctcatcctcg ccccagcaga gcatggcgtc ctccagtgaa acattcatcc
      241 cctgcgcccc gaaaaccgct ttcacgatct gcgcattacg gatatccccg cgctcatcgc
      301 ccagcgggga caccctgtcg aactccatcc acatcatcgc ctcgctcgca ctcagactgt
      361 ggcccagttc ggataaggtg cgccccagac ggagcgcaag tcgcatcaga aagcgaattt
      421 ccgggcgggc tacttttttc tggccgactc tgcatcagcg atcagttcca gtgcctgacg
      481 cagcaaccgg gcatgtaccg gaccatagac ggccagcacc tgctcacggt cgtccggagt
      541 gaacacccgc tgcagatccg tatcacacag gacatcgcag aacagcgtca catccgcttc
      601 caggttacgg cgggttttcg ccaccaccga cagggtatcg tcatcctctc catcaccatt
      661 gagcacttcc tgccacagat accaggcctc tgccgaaggc tcccgcagca ccacgctgac
      721 attaccccat tccggcacct tcaccgtttt atgacgaaac cctgacagtc tggccagcgc
      781 cagcgttttc agatcctttt tcatgatgac ccatcccctt atccggcggc tgcgctcact
      841 gtcacggtgc attcaacaga cgtcacactc tgtgctttct ctgccgaatc ggtcaccacg
      901 caggtatatt tccccgcatc agcggactgc gcacctggct tactgaaggt gtctgtcgtc
      961 tgcccgtcaa ccggctgacc atccttcttc caggcgtatt tatacggcgg cgttcccccg
     1021 ttggcactga ctgacattgt cagcagcgca cctgtattca cggtaagtgt tttatccaga
     1081 tttttcacaa acgccagcgg taccacaaag gacaccggtt tgcctttcat acgcagtgaa
     1141 aacgttgctg ccaccacgcc gttggtaccg gatgaccagg tgtgctgacg cacttccgcc
     1201 aggaacttaa agcccttacc ggacggaaac agcaccttaa acgcatacaa cgcgtcattg
     1261 tcataggcat cacgcagggc gttctgggcc tgattcagat aaaaattacc cgacatggaa
     1321 atctcagacg acgcccccag accgttgatg ttctcctgct ctgtggagca gagcgtggtc
     1381 acatcaatat cctgtttctg accggcggtg aactggactt ccttgatggt gcaatccagg
     1441 cgcagatatt ccgccttatc catagtttca gcagtcgccg gggcagatga aatcatcacc
     1501 tgcgtcagct gtgagcgttc atacaaagca gacattctgc ctcctgataa taaaaaaccc
     1561 gcacgcggcg gggtatgggt tttgtag
//
LOCUS       DAZGOB010000333         1582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGOB010000333 DAZGOB010000000
VERSION     DAZGOB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.335"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1431
                     /locus_tag="ACHLIF_005333"
     CDS             <1..1431
                     /locus_tag="ACHLIF_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9609120.1"
                     /translation="HPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRT
                     DDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMS
                     LSRKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLA
                     EKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQV
                     EPEYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRL
                     PGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYN
                     LYQLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATAC
                     GHAGIGGGINAAITYSETKSLPASGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTE
                     SVGIGAGVDASVMTLACRTWQECWVN"
     gene            1443..>1582
                     /locus_tag="ACHLIF_005334"
     CDS             1443..>1582
                     /locus_tag="ACHLIF_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HFO9609121.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQTGKYRDIYK
                     ITS"
BASE COUNT          403 a          348 c          484 g          347 t
ORIGIN      
        1 cacccggaca gtaccctcac ggcgtggccg gataaccgca tcgcagagga tgcgcactat
       61 gtttaccact atgatgaata cggcagactg acggagaaga cggaccttat cccggcgggt
      121 gttatccgga cggacgacga gcggacccac cactaccact acgacagcca gcaccgtctg
      181 gtgttctaca cgcggataca gcatggcgag ccactggtcg agagccgcta cctctacgac
      241 ccgctgggac ggcgaatggc gaaacgggta tggcggcggg agcgtgacct gacggggtgg
      301 atgtcgctgt cgcgtaaacc ggagatgacg tggtacgggt gggacggcga ccggctgacg
      361 acggtacaga ccgacaccac gcgtatccag acggtatatc agccggggag cttcgcgccg
      421 ctcatccgca tcgaaacaga caacggcgag cgggagaaag cgcagcgccg cagcctggcg
      481 gagaagctgc agcaggaagg gagcgaggac gggcacggcg tggtatttcc ggctgaactg
      541 gtgcggctgc tggacaggct ggaggaagaa atccgggcag accgcgtgag cagtgaaagc
      601 cgggcgtggc ttgcgcagtg cgggctgacg gtggagcaac tggccagaca ggtggagccg
      661 gaatacacac cggcgcgaaa agttcatttt taccactgcg accaccgggg cctgccgctg
      721 gcgctcatca gcgaagacgg caatacggcg tggcgcgggg agtatgatga atggggcaac
      781 cagcttaatg aagagaaccc gtattacctg caccagccat accgtctgcc ggggcagcag
      841 catgatgagg aatcagggct gtactataac cggaaccggt actatgaccc gctacagggg
      901 aggtatatta cacaagaccc cattgggctg gcggggggat ggaacctgta taattaccca
      961 ctgaatccga taataaggat ggatcctttg ggtttgtata atttatatca attattatat
     1021 gatgtttggc atgatgattc atatggaaca tcatcaattg atattactgg cagtggagat
     1081 ctaatatcat taggtggtca tgcaggactt ggcgttgcgt ttgctaaaaa gaaaggtgaa
     1141 atgttatctg atatttgtat ttatgctaca gcatgcggac atgcaggaat tggtggtggg
     1201 ataaatgcgg ctatcacata ttctgagacc aagtctttac ctgcatcggg agtcagcaat
     1261 tcagtaggtg taacggttgg cggcggagtt ggggggcatt ttgcgtatac ttatgtagtg
     1321 gatgttgata atccagaatc atcgacagaa tctgttggta tcggtgcagg tgttgacgct
     1381 tcagttatga ctctggcttg tagaacgtgg caagaatgct gggtcaatta acaggaggaa
     1441 atatgaaaaa atatatattt ttagttttat ttttattgtg tggttgttct ccagtggaca
     1501 tagagccagc tagacaagaa gttaatatct ttcgtggttt atatcagaca gggaaatacc
     1561 gtgatatata taaaatcact tc
//
LOCUS       DAZGOB010000334         1574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGOB010000334 DAZGOB010000000
VERSION     DAZGOB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.336"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..354)
                     /locus_tag="ACHLIF_005335"
     CDS             complement(<1..354)
                     /locus_tag="ACHLIF_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609122.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHCPGEV"
     gene            complement(645..1220)
                     /locus_tag="ACHLIF_005336"
     CDS             complement(645..1220)
                     /locus_tag="ACHLIF_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO9609123.1"
                     /translation="MPLTSAIASNSFSTGMQVLRAQMAASGGGEITVGGQTVRITYSE
                     TDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCK
                     FSVNTESLQCPSEAVRCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHP
                     LTREPITSSMIVSQEQCIYDQTKGNFVIKDK"
     gene            complement(1333..1572)
                     /locus_tag="ACHLIF_005337"
     CDS             complement(1333..1572)
                     /locus_tag="ACHLIF_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9609124.1"
                     /translation="MCGCGKHDGREHVQSLTAQLRLGPADILESDENGIIPEQDRVIT
                     QVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          435 a          440 c          268 g          431 t
ORIGIN      
        1 cacctcacca ggacagtgaa gtttttcaat atcaacagta aaggtacatt ccgatatttt
       61 ggcctgcaaa gagttctctt gtcgctgcgc caccattcca ggaagcacgc ttaacaccct
      121 gtttctgagc gcatcaactc catcattaaa accaatattc aataattctg cataaaccag
      181 tcccagccct tcactttctg ctaaaaaacg gcctgtatct ggcatataag aaactctaac
      241 tctctcccct cccaggagaa atcctccaat ctgcccttcc attacctgct gacgcatttc
      301 cgccatctca ttcgcgaatg aagtcgatga tatatatgat actaatccag gcattaccat
      361 attccttcat acagataaat atttcctctg ggcttcataa caaacatctc cctgacatga
      421 caacaaaaac cggagccgga ctccggtttt tgtgaatccg tcggctattt catcccgcca
      481 atatttcccc gtcagcacgc cagatttgca gcggcctcac cactcactgt atatgctttt
      541 tagctgcatc cagtacacca attaccacat ccttataccc tgaacatctg tagcatttca
      601 tataagcaac tggcagttaa catatagctt gggtaatatt tatttcactt atcctttatg
      661 acaaagtttc ctttggtttg gtcataaata cattgttctt gacttacaat cattgatgac
      721 gttattggtt ctcgtgttag cgggtgcttc ccaccgtcgc gaaccaaatg agaaaaagaa
      781 accgaatcaa ataaggtaca aaccaaagaa ccttctgaat ttttaacaaa cacaccttct
      841 tctggtttat ccagtataat tgggcatcga accgcctcgg atggacactg aaggctctcc
      901 gtattaacag aaaacttaca ttgtgatatt ttcccctcaa tatcaccatg tgattgtgtg
      961 ttacctgaac ctgaaaccat tcttaacatt atatccctga gcgcttcagg accaccatta
     1021 agccctgtaa gtaataatcc agaaagcaaa ctgttattgc ccccactcgc cagaaagcga
     1081 ccatccgttt cactatatgt gatacggacc gtctgcccgc ctactgtaat ttctccaccg
     1141 ccactggcgg ccatttgagc acgaagaacc tgcattccgg tggaaaatga attggatgca
     1201 atagctgatg ttaacggcat gacaaaactc cctatttaaa tattcattcc aaacacaggg
     1261 agtcataaca aacagccccc atacatgaca acaaaaaccg gagccggact ccggttttgt
     1321 gaagctgtcg ggttacttca tcccgccaat attttcccac gtcccgtcag cacgcagaat
     1381 ttgcagcggt cttaccacac actgtatctg ctttttatcc gcatccagta tcaccacctg
     1441 cgtgattacc ctgtcctgct ccgggataat accattctca tcggactcca ggatgtctgc
     1501 cggccccagt cgcagttgtg ctgtaagcga ctgcacgtgt tcacggccat catgctttcc
     1561 gcaaccacac agac
//
LOCUS       DAZGOB010000335         1564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGOB010000335 DAZGOB010000000
VERSION     DAZGOB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.337"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            377..526
                     /locus_tag="ACHLIF_005338"
     CDS             377..526
                     /locus_tag="ACHLIF_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO9609125.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            1338..>1564
                     /locus_tag="ACHLIF_005339"
     CDS             1338..>1564
                     /locus_tag="ACHLIF_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9609126.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          431 a          361 c          396 g          376 t
ORIGIN      
        1 aaacacatga gatcaaatac atgagttcat atatgtgaaa taaacacggt gagtgtgatc
       61 agtgggcatg atgagaacgc agtagcaaca gactgcgcta tccctgatag ctggcgtata
      121 acctgcgtat ataaaacgta ccagcaacgc acgtgaacag agtgcattaa gcatccgtcg
      181 ttgacaccga cctggtgagt gacagtctga gagaccggtt aagtggtggt acgggagtaa
      241 tgagtttggg gagggtacca gaatgtcagt ctggtacggt atgctgacag tatcacgtta
      301 attgcagcat aaagtgatga gaggctaatg gaagttaaaa ccagagcagt tccgtcgttg
      361 ttcatcccgg gagacactgg aaaaagtgat ttcccacacg cgttataagc ttactccagc
      421 ggagatggaa gccttcaact ctgcggtcga tcaccgactg gcagaactga caatgaacaa
      481 gctttacgat cgtgttcccg cttcagtctg gaaatatgtc atctgagcgt tcggtaatca
      541 caactgcatc gcgctaggtg ttatccgctt tgtgattctt attggctctt ctcaggttat
      601 taaaagctga atatgttcct gttgtcggga accaggataa taccgggagc gttaagcggt
      661 atagcgacaa caaagaattc gaaatcaaaa gggaatggtg ccggaggcgt gggcggtaat
      721 aggctgattt tcaggtatta ccggcttaca acgtgtggtt gatattgagg ggctgtgagt
      781 ttcgcgtatt tgtcagaaac ccagatgagt tttcctgtat gggacgaaat aaggcagcgg
      841 caatacctgg aaaatttaat atggtatgaa ggagaacctg tcggttacgc ttaatataca
      901 cggtacactg tcagctcagg ggatacatga accgttatct caccagctac acagtataca
      961 cacactgacg ctgacagtag tcagcaggac cacaataatc agcgccagca gtatggtaac
     1021 gatacggtct gtattccggt agtagccgaa ggggtcgaca gaaccacaat acgggcaaca
     1081 cactgtatta ttagtaatcg cgtttccaca gtccctgcac ttcttttgcg tatcaccata
     1141 actccctcct cacagcctta cccgtaaccg gttttttaca ttaaaaaaca acgctgaaaa
     1201 ttcaaccgtc agtccggaga cgaccgttcg ggttatcaca gagtgcctga gacagtgtcc
     1261 tgccggaggt cacttcaggg actctgcgca ttttttacga cgtggctatt ccgtatgaac
     1321 catacggaga tttaaccatg acctacaaat acaacccctt ctggcagcaa cgtattcgtg
     1381 agacggtgcg gcacgcactg gatgttcatc cccgtctgac ggcattgcgg gttgatctgc
     1441 gtctcccgga tgtaccggca gcaacggacg cagctgtgat atcccgcttc atcaatgccc
     1501 tgaaagcccg aatcgacgct taccagaaac gtaagcatcg ggaaggtaaa cgcgtgcatc
     1561 ccac
//
LOCUS       DAZGOB010000336         1559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGOB010000336 DAZGOB010000000
VERSION     DAZGOB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.338"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1559
                     /locus_tag="ACHLIF_005340"
     CDS             <1..>1559
                     /locus_tag="ACHLIF_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9609127.1"
                     /translation="EVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALM
                     KMPEVNAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVV
                     DLARARANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGS
                     IHRWAKKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNK
                     YICEYLTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVERE
                     GPFYFLFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTA
                     EDSEQPEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQ
                     TRDSVNQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKACTACGQTGGGN
                     CPDCGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGE
                     TADHPIKVNGH"
BASE COUNT          503 a          360 c          399 g          297 t
ORIGIN      
        1 gaagtacaga acgctgtcat ggttaagttc gacacatgtg aaaacatcac cgttgatatg
       61 gttattagcg cacaggaatt gttgcaggaa gacatggcaa cattcgacgg acatatcgtt
      121 gaagcgttga tgaaaatgcc agaagttaac gccatgtatc cggagcttaa gttgcacgcc
      181 attgggtggg ttaagcataa atgtattcct ggtgctaaat ggcccgaaat tcaggcagag
      241 atgcgcatct ggaaaaaacg tcgcgaaggt gaacgcaagg aaaccggaaa atacacgtct
      301 gttgttgatc tcgcccgcgc cagagccaat caacagtaca ctgaaaattc aacaggaaaa
      361 atcagcccgg tcattgctgc cattcatcgc gaatacaagc agacatggaa aacactggat
      421 gacgaactgg cctacgctct ctggcctggt gatgtggatg ccggaaacat tgacggcagc
      481 atccatcgct gggcaaaaaa agaagttatc gacaacgacc gcgaagactg gaagcgtatc
      541 tcggcatcaa tgcgcaaaca gcctgatgcc cttcgctacg accgccaaac tatttttggc
      601 cttgtccgtg agcgtccgat cgacattcac aaagatcccg tagcactgaa caaatatatc
      661 tgcgaatacc tgacgacaaa gggcgtgttt gagaatgaag aaacagacct gggcactgtt
      721 gatgttctcc agtcatcaga aacacaaact gatgcagtgg aaactgaggt atctgatatc
      781 ccaaaaaatg aaaccgcgcc ggaagctgaa ccatctgtag agcgtgaggg gccgttctat
      841 ttcctcttcg cagataagga cggagaaaaa tacggtcgcg caaacaaact ttctggtctg
      901 gataaggcac tggctgctgg cgccactgaa atcacaaaag aagaatattt tgcccgaaaa
      961 aatggcacat acacgggctt accgcaaaat gtagataccg ctgaagattc agaacaacca
     1021 gagccgataa aagttaccgc tgacgaagta aacaaaatta tgcaggcagc caatatcagc
     1081 cagcctgacg ccgataagtt gcttgctgca tcacgtggtg aatttgttga agggattagt
     1141 gacccgaatg atccgaaatg ggttaagggg atccagaccc gcgattctgt gaaccagaac
     1201 cagcatgaat cggaacggaa ctaccaaaaa gcggaacaaa acagtccaaa tgcgttacaa
     1261 aacgagccag aaacgaaaca gcctgaacca gtggcgcaac aggaagtgga aaaagcctgc
     1321 accgcctgcg gtcagaccgg cggcggcaac tgccctgatt gtggcgcggt gatgggcgac
     1381 gcaacatacc aagaaacatt cgatgaagag tatcaggttg aagttcagga agatgatccg
     1441 gaggaaatgg aaggcgctga acatccacac aaggagaaca ctggcggcaa tcagcatcac
     1501 aatagcgata atgaaactgg cgagacggca gatcacccaa ttaaggtgaa cggtcatca
//
LOCUS       DAZGOB010000337         1557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGOB010000337 DAZGOB010000000
VERSION     DAZGOB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.339"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..375
                     /locus_tag="ACHLIF_005341"
     CDS             <1..375
                     /locus_tag="ACHLIF_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9609128.1"
                     /translation="GAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
     gene            650..1219
                     /locus_tag="ACHLIF_005342"
     CDS             650..1219
                     /locus_tag="ACHLIF_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO9609129.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
     gene            1219..1368
                     /locus_tag="ACHLIF_005343"
     CDS             1219..1368
                     /locus_tag="ACHLIF_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609130.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVILVAIFFSTAWRVLTL
                     SGLVG"
     gene            1376..>1557
                     /locus_tag="ACHLIF_005344"
     CDS             1376..>1557
                     /locus_tag="ACHLIF_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9609131.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     ELANATITDMQQRQRDV"
BASE COUNT          422 a          322 c          431 g          382 t
ORIGIN      
        1 ggcgccatca tggtggatgg taagcctgtg attcctggca tgaagctgtc gaaggaaaaa
       61 tgcgaccggg ttaacgctat cgaacgggat aaggcgctgg catgggtgga gcgtaatata
      121 aaagttccac tgaccgagcc acaaaaagca ggtatcgcgt cattttgccc ctataacatt
      181 ggccccggta agtgtttccc gtcgacgttt tataagcggc ttaatgcagg cgatcgcagg
      241 ggagcgtgtg aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgt
      301 tcaaacaact gttacggtca ggtatcaaga cgtgaccagg agagtgcgct ggcatgctgg
      361 ggtatcgaca ggtaagcaga atattttgct gaaaaatgac gttggccaac gcgggtagac
      421 aacacgaaat cctgcgaact ggcaaaatgt aagtgaataa agtcaacaag attgtttcat
      481 gaagaggcac cgtaatggtg cctttgtcat ttctgcgctt cgcacaagcg taaataaacc
      541 aaagaacctt tcaggatgag ccctggtgga taaccggcag tggtctggtt aaccctcttt
      601 gggctggtta ttcctgtgcg cagggttcat cactaaaagg aaataaccga tgaatatgat
      661 gaccgtgccg tttcacggcg attctcttta tgtggttaac cataacggcg aaccatacgt
      721 tcccatgaaa cctgtcgttg cggggatggg gctggcctgg caatcacagt tggctaagtt
      781 aagacagcgt tttgcgtcaa ctataacgga aatcgttatg gttgctgagg atgggaaacg
      841 acgcaatatg gtgtccctgc cgcttcgaaa acttgcaggc tggttacaaa ccatcaatcc
      901 caacaaagta aaacccgaaa tccgcggcaa ggtaatccag tatcaggaag agtgtgacga
      961 tgttctctat gaatactgga cgaagggttt tgtcgttaat ccccgtcgaa tgagtgtgat
     1021 ggaagaactc aatcaggctt gcgctgacat gaaacgggat aaaaacattg ccagtgtgtt
     1081 tgctaccggg ctgaatgagt ggaaacaggt taaatccgcg catgtatcaa aaatccgcac
     1141 attgataaac gaagcgaatc tgctgattga ttttgtcctg gctgatacag acaaagggaa
     1201 aataacaaag gcggattgat ggagtggtgg ctaatgatat cggataaact cataacgctg
     1261 gcgaagatcc tctgtgtaat cgtcggcatt tcatttttag tcattctggt tgccattttc
     1321 ttttccaccg cttggcgagt cctgacgtta tcgggactgg tggggtgaaa gagagatgaa
     1381 ccgtgttctg tgtgtggtga ttattgtcct gctggtagcc tgtggtgcac ttagtctggg
     1441 gctgaatcat taccgcgata acgccataac ctacaaagag cagcgcgata aaaaagtcag
     1501 tgagctggag ctggcaaatg caaccattac tgatatgcag cagcgccagc gtgatgt
//
LOCUS       DAZGOB010000338         1551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGOB010000338 DAZGOB010000000
VERSION     DAZGOB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.340"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1551
                     /locus_tag="ACHLIF_005345"
     CDS             <1..>1551
                     /locus_tag="ACHLIF_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9609132.1"
                     /translation="VYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEICYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLKAQTDAAGRTTEYSPDVVTGLTTPDGRASAFYYNHHNQLTSATGPDGLELRRE
                     YDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCS
                     GYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEYNIAGDLTA
                     VIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLD
                     RLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWYYDEADRLTHRTVKGETAERWQY
                     DERGWLTDISHISEGHRVAVHYGYDSKGRLASEHLTVHHPQTEALLWQHETRHAYNAQ
                     GLAKRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETLRRFGRYELTTA
                     YTPAGQLQR"
BASE COUNT          381 a          456 c          470 g          244 t
ORIGIN      
        1 ggtgtatgac cgtagtggca aacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgggccg accggaaatc tgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagt tacacgtatc agtatgaaaa
      181 agaccgcatc accatcaccg acagcctgaa ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcg gtgggcaggc ttaaggcaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcaccac gccggatggc agggcatcgg cgttttacta
      421 taaccaccac aaccagttaa cgtcagccac cgggcctgac gggctggaat tgcgccggga
      481 atatgatgaa tccggccgtc tgattcagga aactgcccct gacggcgata tcacccgcta
      541 ccgttatgat aatccacaca gtgacttacc ctgcgcaacg gaagatgcca ccggcagccg
      601 gaaaaccatg acgtggagcc gttacggtca gttgctgagc ttcaccgact gttccggtta
      661 tgtaacccgc tatgaccatg accgttttgg tcaggtgacg gcggtgcacc gcgaggaagg
      721 cctgagccag taccacgcat acgacagccg tggacagtta actgcggtga aagacacgca
      781 gggccatgaa acgcggtatg aatacaacat cgccggtgac ctgaccgccg tcattgcccc
      841 ggacggcagc agaaacggga cacagtacga tgcgtgggga aaggccatct gtaccacgca
      901 gggcgggctg acgcgcagta tggaatacga tgctgccgga cgggtcatcc gcctgaccag
      961 tgaaaacggc agccacacca ccttccgtta cgatgtactc gaccggctga tacaggaaac
     1021 cggctttgac ggccgcacac agcgttatca ccacgacctg accggcaaac ttatccgcag
     1081 cgaggatgag gggctggtca cccactggta ctatgacgaa gcagaccgcc tcactcaccg
     1141 caccgtgaag ggtgaaaccg cagagcggtg gcagtatgac gaacgcggct ggctgacaga
     1201 catcagccat atcagcgaag ggcaccgggt ggcggtgcac tatggttatg acagtaaagg
     1261 ccgcctcgcc agtgaacacc tgacggtgca tcatccgcag acggaagcac tgctctggca
     1321 gcatgagacc agacatgcgt acaacgcgca gggactggcg aaacgctgta taccggacag
     1381 cctgcccgcc gtggaatggc tgacctacgg cagcggttac ctggcaggca tgaaactcgg
     1441 cgacacaccg ctggtggagt acacccgcga ccgcctgcac cgggaaacgc tgcgccgctt
     1501 cggccgttat gaactcacca ccgcttatac ccctgccggg cagttacagc g
//
LOCUS       DAZGOB010000339         1533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGOB010000339 DAZGOB010000000
VERSION     DAZGOB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.342"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..901
                     /locus_tag="ACHLIF_005346"
     CDS             <1..901
                     /locus_tag="ACHLIF_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9609133.1"
                     /translation="ATNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDA
                     KNLFLIASNHLPVDELHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLA
                     GKKELYDFTPESIYRAMTIFDILQNKSDIQTLKTECYCLLAECHMSLALHGKSELELA
                     AQKALELLDYVSDITTVDGKILAIMGLITGLSGQAKVSHILFEQAKIHPTDIASLYYY
                     RALVHFHNEKIEEARICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETE
                     SESHRVIIDNILKLKQLTRICMR"
     gene            1069..1287
                     /gene="ygeI"
                     /locus_tag="ACHLIF_005347"
     CDS             1069..1287
                     /gene="ygeI"
                     /locus_tag="ACHLIF_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFO9609134.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            1370..>1533
                     /locus_tag="ACHLIF_005348"
     CDS             1370..>1533
                     /locus_tag="ACHLIF_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO9609135.1"
                     /translation="MRSPKVKFLTIFTFCIFITKMSFASNSCSNEAGTMFRIEPNLIK
                     AIALVESNLK"
BASE COUNT          572 a          272 c          241 g          448 t
ORIGIN      
        1 tgcgacaaat ttttgtaatg atcacatatc tcaaaattca ttcttgagca gattcaagcc
       61 agattatttc gttacaggaa gaataaacca gaataatgca gtgaacactt tatacattga
      121 gttgatcgac gctaaaaacc ttttcctcat cgccagtaat catctccctg ttgatgaact
      181 acataataca tcacaattta ttatagataa tatccttcaa acggtacata aaccagaacg
      241 atctgtaaga ttagctaagc aggaccaagg atataagaat cattatttat cagatgaaat
      301 gttagccgga aagaaagaac tttacgactt cacccctgaa agcatttaca gggccatgac
      361 tatatttgat atattacaaa ataaaagtga tatacagacg ctaaaaacag aatgttattg
      421 ccttctagcg gaatgccata tgtctttggc acttcatgga aaaagtgaac ttgaacttgc
      481 tgctcaaaaa gcattagagc ttttagatta tgtatcagac ataaccactg tcgatggaaa
      541 aattttagct attatgggac tgataactgg tctgtctgga caagcaaaag tatctcatat
      601 cttatttgaa caggctaaga tacacccaac tgatatagcc tctctctact actatagggc
      661 actagtccac tttcataatg aaaaaattga agaggcaagg atttgtatag acaaatcact
      721 acaactcgaa cccagaagac gaaaagcagt tgtgataaaa gaatgtgtag atatgtatgt
      781 gcctaacccg ctcaaaaaca acattaaact ctactataaa gaaactgaga gtgaaagcca
      841 tcgagttata attgacaaca ttttgaaatt aaagcagctg acgagaattt gtatgcgata
      901 aatttttatt gacgcaattt tattatttag atgtatgcaa cttgttattt aaacaacaga
      961 gataaaaata tctaagatat tcaccttatt gcaagatatt taaaatgctc tagagttaaa
     1021 agtatgatgt aaagaccatt ggcctggaca attaaaaaag ggagctttat gactaatcca
     1081 atcggtatca ataatttatc tcaaagttca aatatagcga atgcaacggg tgatgaagtc
     1141 gtatcattag ataaacacat caacaccagt gctactgata cagatcaaat acaagcattt
     1201 atagtttcaa catggatggc gccttttcaa aatgatatgt attcagaaga taatcctatc
     1261 tcaccttact ataaaattga atggtagacg attgaccaaa taaatcataa cgagatactt
     1321 ttataatagt atctcgtccc tttcattcaa tcctcacaca tgaaaaaata tgcgctcacc
     1381 aaaagttaaa ttcttaacca tcttcacgtt ttgtatattt attacaaaaa tgtctttcgc
     1441 cagcaactct tgctctaatg aagcaggaac aatgttcaga atcgaaccaa atttaattaa
     1501 agctatcgct cttgttgaaa gcaatctaaa aaa
//
LOCUS       DAZGOB010000340         1486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGOB010000340 DAZGOB010000000
VERSION     DAZGOB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.343"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1196
                     /locus_tag="ACHLIF_005349"
     CDS             <1..1196
                     /locus_tag="ACHLIF_005349"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9609136.1"
                     /translation="LTDAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLP
                     VSGTLVSRTRALQPYSGMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCA
                     DIIARVRDIKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAAL
                     EKQGVEITLIYSGSHKVDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQA
                     VLDTEAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTV
                     SATASQADVTGVVPAMEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQ
                     ARVLAETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDL
                     LNTPV"
     gene            1206..>1486
                     /locus_tag="ACHLIF_005350"
     CDS             1206..>1486
                     /locus_tag="ACHLIF_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO9609137.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKASAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAAS"
BASE COUNT          312 a          435 c          474 g          265 t
ORIGIN      
        1 gcctgacgga tgcagtatcc ggcgacagcc tgactgccgg agaggcaccc gcggcgctgg
       61 cgttatccgg tgatgatgac ggaccacgac aggcccggag ttatcaggtc atgaacggca
      121 tcgccgtgct gccggtgtcc ggtacgctgg tcagccggac gcgggcgctg cagccgtatt
      181 cggggatgac cggttacaac ggcattatcg cccgtctgca acaggctgcc agcgacccga
      241 tggtggacgg cattctgctc gatatggaca caccgggcgg aatggtggcg ggagcatttg
      301 actgtgctga catcatcgcc cgtgtgcgtg acataaaacc ggtatgggcg ctggccaacg
      361 acatgaactg cagtgcaggt cagctgcttg ccagcgccgc ctcccggcgt ctggtcacgc
      421 agaccgcccg gacaggctcc atcggcgtca tgatggctca cagtaattac ggtgctgccc
      481 tggagaaaca gggcgtggaa atcacgctga tttacagcgg cagccataag gtggatggca
      541 acccctacag ccatctaccg ggtgatgtcc gggaaacact gcagtcccgg atggatgcaa
      601 cccgccggat gtttgcgcag aaggtgtcgg catataccgg cctgtccgtg caggctgtgc
      661 tggataccga ggctgcagtg tacagcggtc aggaggccat tgatgccgga ctggctgatg
      721 aacttgtcaa cagcaccgat gcgatcaccg ttatgcgtga tgcactggat gcacgtaaat
      781 cccgtctctc aggagggcga atgaccaaag agactcaatc aacaactgtt tcagccactg
      841 cttcgcaggc tgacgttact ggcgtggtgc cagcgatgga gggcgaaaac gccagcgcgg
      901 cgcagccgga cgtgaacgcg cagatcaccg ctgcggttgc ggcagaaaac agccgcatta
      961 tggggatcct caactgtgag gaggctcacg gacgcgaaga acaggcccgc gtgctggcag
     1021 aaacccccgg tatgaccgtg gaaacggccc gccgcattct ggccgcagca ccacagagtg
     1081 cacaggcgcg cagtgatact gcgctggatc gtctgatgca gggggcaccg gcaccgctgg
     1141 ctgcaggtaa cccggcatct gatgccgtta acgatttgct gaacacacca gtgtaaggga
     1201 tgtttatgac gagcaaagaa acctttaccc attaccagcc gctgggcaac agtgacccgg
     1261 ctcataccgc aaccgcgccc ggcggattga gtgcgaaagc gtctgcaatg accccgctga
     1321 tgctggacac cgccacccgt aagctggttg cgtgggatgg caccaccgac ggtgctgccg
     1381 ttggcattct tgcggttgct gctgaccaga ccagcaccac gctgacgttc tacaagtccg
     1441 gcacgttccg ttatgaggat gtgctctggc cggaggctgc cagcga
//
LOCUS       DAZGOB010000341         1466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGOB010000341 DAZGOB010000000
VERSION     DAZGOB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.344"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..270)
                     /locus_tag="ACHLIF_005351"
     CDS             complement(<1..270)
                     /locus_tag="ACHLIF_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609138.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTS"
     gene            complement(365..571)
                     /locus_tag="ACHLIF_005352"
     CDS             complement(365..571)
                     /locus_tag="ACHLIF_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9609139.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            1328..1453
                     /locus_tag="ACHLIF_005353"
                     /pseudo
     CDS             1328..1453
                     /locus_tag="ACHLIF_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
BASE COUNT          379 a          341 c          414 g          332 t
ORIGIN      
        1 actggtgcat aaattcacta tcagatcatc atccaggggc ggtgtggccc gtttgtcagg
       61 gccataaatc agccggtgca ggctctgggg tacgatggca atctccggtt tttcaccatt
      121 gaatgacacc caaccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      181 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcca
      241 ttcgtagcgg gtctgatact gttgtttcat tttcatattc ctcacgggga tataacaggc
      301 agcagaatgc cgccggcagg ccggagatta cgtctgctgc gggtgaacgg ataacggtca
      361 gaaattacgg acgggactgt gcaatacgcg cctgcagcca ggcttccact tcacttttca
      421 gccagcggga gctgcgctcc attttgatgg gggcaggaaa gcccccatct ttgatgagtt
      481 tgtaaaacca cttatcggtt aagccggtaa gttgagtgat aaacgccatg tcgaccatct
      541 ggtcatccat cagcgaaaca ggggtagcca tgagaatgtt cctccggatg ttgttaaacc
      601 agccggtgtg gtcatgaaaa cagcgcgcca ccttcactgg cataacaacc ggggagaaac
      661 gtaaaaaata atcgggaatg tggctgtccg gagataaacg gggggattgc gatggcagag
      721 ccgtagcgga atcccgggtg gttgttgcga aaagatgctt ccgcacgcac gcgcgataaa
      781 ggagggagga ctttcgtgag acaaggggaa aactagcgtt ctgataattg cagagttgtt
      841 ttcggaggtg gttacagtga gtcagcccgt tcctggcgag gaggagactg atatcaccga
      901 tgggataagg ctgtctgatt ggtattataa atataagaag tcatcagaga gtaacgtaaa
      961 ggatttgcca gagtaagcta agagagtaaa tctctccggt aacagagcgc tgtgaatcgt
     1021 tcatgtatca gagtgcagat acatgaacgc tgatgcgtaa aatcagcagt gacacgccag
     1081 ctccccctgt gcccattcct gtataagaca tgaactgagc gtattaatgc tcctgccctg
     1141 agactgagcc ggtgagtgag gggctgagaa atccctcttg acactgctct aaaccagaaa
     1201 tacttccccg gttgttgcgg tgcgtccgga tccccgataa cggaacacta aggctgtatt
     1261 gcgggacacc tagtaagtgg cagcccgtaa tacgtcagga gacgcgggaa aagtgaattc
     1321 ccacacgcgt tataaactta cccttgcgga gctggaagcc tttaactctg ccgttgacaa
     1381 ccggctggca gaactgacaa tgaacaaact ttacgatcgc gcgccggctt ccgtctggaa
     1441 atatgtcacc tgaatattga agcagc
//
LOCUS       DAZGOB010000342         1466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGOB010000342 DAZGOB010000000
VERSION     DAZGOB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.345"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..507
                     /locus_tag="ACHLIF_005354"
     CDS             <1..507
                     /locus_tag="ACHLIF_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609140.1"
                     /translation="AITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGKAFPRN
                     NSYASGTVTVTVYDDQGFDRQIIIPPVLFRGTKHQNFNSPNQQSYWYSTCKLQVLKNG
                     VEIFHEPATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYISDLLVVVMKK
                     STAGISIS"
     gene            576..1199
                     /locus_tag="ACHLIF_005355"
     CDS             576..1199
                     /locus_tag="ACHLIF_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9609141.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGSASGDWRTDGFIVGVGYKF"
     gene            1264..>1466
                     /locus_tag="ACHLIF_005356"
     CDS             1264..>1466
                     /locus_tag="ACHLIF_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO9609142.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFP"
BASE COUNT          354 a          338 c          434 g          340 t
ORIGIN      
        1 gcaattaccg cgaataccgg cacgctcaat aatgtcacca ttaacgagaa ctgtgtcatc
       61 agagggaaac tgtctgcaaa ccagattgaa ggcgacctgg tgaagacggt ggggaaagcc
      121 tttccccgga ataacagtta tgccagcggg acggtaaccg tcacagttta cgatgaccag
      181 ggattcgacc ggcagattat cattcccccg gtgctgtttc gcgggacgaa acaccagaat
      241 ttcaacagcc cgaatcagca gtcgtactgg tattccacct gtaagctgca ggtgctgaag
      301 aacggggttg agattttcca tgaaccggca acggatgtca gccgggtgtt ctcatcggtg
      361 atagatatgc cggcagggcg gggtcatgtc accctgacgt ttaatgtgtc gtcggccggt
      421 gcgaacaact ggacgccgac aacgtacatc agtgatttac tggttgtggt catgaaaaaa
      481 tccacggcag ggatcagtat cagctgacgg tttattaacc cggacgggca cctcaggagg
      541 tgcctttttt attgactgaa aacaaagagg taatcatgcg gcatttatac gcaacgatat
      601 tattgtttac taccctgctg gcaggaattg cctttcctgc acaggctgaa agcggacacg
      661 gtgcattttc cgtgggatat gctcaggttc acccgggcgg cgtaccggca ttgtccggta
      721 ccggtgcgcg tgcaggtgat ttaaaaggga ttaatgtgaa ataccgttat gagttcacgg
      781 atcacctggg cggcattgtc gcgctgagtt atgcatcggt gaagaaaagt gacacgatga
      841 agacgggtga aaataccttc cattatgaaa gcctgcgcgg tcgttatgtc agtctgatgg
      901 ccggccctgt ctggcagctc agtgagcggg tcagtctcta tggcatggcc gggatggcgt
      961 acacccgctg gtctgacagt gttcaggatt accggcgtga tgaagtgaaa ccggggtatg
     1021 tgaaggagac caccaccgcc agtgatggcc atactgcgcg tcatctgtcg ccggcctgga
     1081 atgccgggat tcagttcagt cccgtagaga cggtggttat tgaccttgct tatgaaggtt
     1141 ccgccagtgg cgactggcgc actgacggtt tcatcgtggg tgtcggttat aagttctgat
     1201 tagccaggta acacagtgtt atgacagccc gccggttcag gcgggctttt ttgtggggtg
     1261 aatatggcag taaagatttc aggtgtactg aaagacggca caggaaaacc ggtacagaac
     1321 tgcacaatcc agctgaaagc aaaacgtaac agtaccacgg tggtggtgaa cacgctggcc
     1381 tctgaaaatc cggatgaagc cggacgttac agcatggacg ttgagtacgg tcagtacagc
     1441 gtcattctgt tggtggaggg atttcc
//
LOCUS       DAZGOB010000343         1463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGOB010000343 DAZGOB010000000
VERSION     DAZGOB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.346"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..231
                     /locus_tag="ACHLIF_005357"
     CDS             <1..231
                     /locus_tag="ACHLIF_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9609143.1"
                     /translation="GVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVPY
                     GEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
     gene            477..>1463
                     /locus_tag="ACHLIF_005358"
     CDS             477..>1463
                     /locus_tag="ACHLIF_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9609144.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSQGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYL"
BASE COUNT          365 a          370 c          462 g          266 t
ORIGIN      
        1 ggtgtggccc agatgctggc cccgaaggca aagacgccgg agtacaaaag tacggataac
       61 ggtaaacaga acacgtattt ttcgtcactg gacaacatga ttgcccaggg gaacccgatg
      121 ccggtgcctt acggtgaaat gctggttggc tcccggcgaa tctcccagga catcagcacc
      181 cgtgatgaag gtggtggcgg gaaggtggtg gttatcgggc ggcaggggta aagcataaaa
      241 aaatcccgca gtgtatggag gctgcgggaa cagaaaatga agattaacca cagggggttt
      301 tgtttttatt ggcccgaaaa aactgtaacg cccgggaatg atatctgcca cggggcgtac
      361 agaaaatgtg aagaaattca gaaattttat tccgtcatga cacaggcacc ctccggggtg
      421 cctgtcgttt ttggggcata aacagattca gacatcagac aggagagggg gacagagtgg
      481 gtaaaggtgg cggcagggcg cacacgccgg tagaggcaaa ggacaatctt aagtccacgc
      541 agatgatgag cgtgattgac gccattggtg aagggccgat tgaaggtccg gtgaaggggc
      601 tgcagagtat tctggtgaac aaaaccccgc tgacggacac ggacggtaat cctgtgatac
      661 atggtgtgac agcggtctgg cgcgccgggg agcaggagca gacaccgccg gaaggttttg
      721 agtcatccgg ctctgaaacc gcactgggcg tggaagtgac gaaggcaaag ccggtgacgc
      781 gcaccattac gtccgcgaac attgaccgcc tgcgggtcac cttcggggtg cagtcactgg
      841 tgcagaccac ctcacagggt gaccgtaacc cggcatccgt ccgcctgctg attcagctgc
      901 agcgtaacgg taactgggtg acggaaaagg atgtcaccat taacggcaag accacctcac
      961 agttcctcgc ttcggtgatt ctggataatc tgcctccccg cccctttaac atccggatgg
     1021 tcagggagac ggcggacagc accacggacc agctgcagaa taagacgctc tggtcgtcat
     1081 acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcgattgtg gggctgcagg
     1141 tggatgcgga gcagtttggc ggtcagcaga tgacggtgaa ctaccatatc cgcggtcgca
     1201 tcatccaggt accgtcaaac tatgacccgg aaaaacgcac gtacagcggc atctgggacg
     1261 gcagcctgaa accggcatac agcaacaacc cggcctggtg cctgtgggac atgctgactc
     1321 acccgcgcta cggcatggga aaacgtctgg gggcggcaga cgtggacaaa tgggcgctgt
     1381 atgccattgc gcagtactgc gaccagacgg tcccggatgg tttcgggggc acagagccgc
     1441 ggatgacctt taatgcgtac ctg
//
LOCUS       DAZGOB010000344         1439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGOB010000344 DAZGOB010000000
VERSION     DAZGOB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.347"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1439
                     /locus_tag="ACHLIF_005359"
     CDS             <1..>1439
                     /locus_tag="ACHLIF_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9609145.1"
                     /translation="TPYTLMQAIAAEGDVTTPVLVKPSDGEGTVISATRDTEYYFVVV
                     LAGQSNSMAYGEGLPLPETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDV
                     QDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGAD
                     GTYSDASGASENSTRWGVDKPLYKDLIGRTKAALKKNPKNVLFAVLWMQGEFDFGGTP
                     VNHAAQFGALVDKFRADLADMAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGS
                     YKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRAS
                     HFSSWARRGIISDRLATAILRHAGRVALNAGASSTVSEVRPSSPSGAEATGVTALLSY
                     LASESEGSLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAAL
                     LQKGGQIRCRFKVSGALAANQYVMAFYWPV"
BASE COUNT          339 a          346 c          467 g          287 t
ORIGIN      
        1 acgccttata ctctgatgca ggccattgcg gcggaaggtg atgtcaccac acctgtgttg
       61 gtgaagccgt cggatggaga aggcacagta atcagcgcca ccagagacac ggagtattac
      121 tttgttgtgg ttctggcggg gcagtcaaac agcatggcat atggtgaagg ccttccgctg
      181 ccggagacat atgaccgtcc ggacccgcgt attaagcagc tggcgcgccg cagtacggtg
      241 acaccgggcg gtgcagcatg caaatataac gacatcattc cggcggacca ttgtctgcat
      301 gatgtgcagg acatgagccg ccttaaccat ccgaaagcgg acctgtcaaa ggggcagtac
      361 ggaaccgtgg ggcaagggct gcatatcgcc aaaaaattgc tgccgtttat accggcgaat
      421 gcgggcattc tgctggttcc gtgctgtcgt ggtggttcag cgttcaccac cggagccgat
      481 ggcacataca gtgacgcgag tggtgcctcg gagaattcaa cccgctgggg tgtggacaag
      541 ccgctgtata aggaccttat cggtcgaaca aaagcagcac tgaagaagaa cccgaaaaat
      601 gtgctgtttg ccgtgttgtg gatgcagggg gaatttgatt ttggcggtac gccggtaaat
      661 cacgcagcac agtttggtgc gctggttgat aaattccgtg cagacctggc ggatatggca
      721 ggtcagtgcg tcggtggctc tgctggcggt gttccctgga tatgtggaga tacgacgtat
      781 ttctggaagc agaagaacga atcctcgtac cagacggtgt acggcagcta caaaaacaaa
      841 acggaaaaga atatccattt cgtaccgttc atgacggatg agaacggggt gaatgtgccg
      901 acgaacaaac cggaagaaga cccggacatt ccgggtatcg gatattacgg ttcgaaatgg
      961 cgtgacagct cagccacctg gacgtcacag gacagggcga gccatttcag ttcatgggct
     1021 cgccgcggga ttatttccga ccgtctggca acggcgattt tgcgccatgc gggaagagtg
     1081 gcgctaaacg cgggggcatc atcgacagta tcagaggtgc gcccgtcatc gccttccggt
     1141 gcagaagcca caggcgtcac agcactgctc tcttaccttg ccagcgagtc agagggaagc
     1201 ctgaaagtac agggatggtc agccagtggc ggcagggcag aagtggtcag cgatgcggag
     1261 ggaaccggag gtaaggcagt gaagctgacc aaggaagccg gtaaaagcag ctgggtgctg
     1321 gagtacgccg cgggcaacgg tgcggctctg ttacagaaag gggggcagat tcgctgccgc
     1381 tttaaggttt cgggagcgct ggctgcgaac cagtatgtta tggcgtttta ctggccggt
//
LOCUS       DAZGOB010000345         1419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGOB010000345 DAZGOB010000000
VERSION     DAZGOB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.348"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(57..281)
                     /locus_tag="ACHLIF_005360"
     CDS             complement(57..281)
                     /locus_tag="ACHLIF_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9609146.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIEA
                     CRRRLINEKYSQFVPNKDKHDQSTCSDRFT"
     gene            323..688
                     /locus_tag="ACHLIF_005361"
     CDS             323..688
                     /locus_tag="ACHLIF_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9609147.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSVSETASGMPD
                     PTLPEVSGGHGRADDIIGL"
     gene            980..>1419
                     /locus_tag="ACHLIF_005362"
     CDS             980..>1419
                     /locus_tag="ACHLIF_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9609148.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPAVMS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTH"
BASE COUNT          335 a          324 c          406 g          354 t
ORIGIN      
        1 gttaccataa gaaaaagcga ctttagtggt cgctttttgt gtcatatata agtcgtttaa
       61 gtaaacctgt ctgaacaggt gctctggtcg tgtttgtctt tgttgggtac aaattgagaa
      121 tatttttcat taattaatct tcttctgcag gcttcaataa cccacgctga aaaattttct
      181 gaacctttct ggtcaagagc gatgttaatt tgttcaatca tctggtttgg aaatcggatg
      241 ttgcgggttg ttgttctgcg gggccggttt ttcgatgaca ttttctttcc tctggtgaca
      301 agctatatgg cgaggatttt acatggctgt gcttcgtacg ttaccgggca gaatcaaaac
      361 tctgaacacc cggcgggtga atattctgaa gggtgaacag cgtcgggtca gtggcagtgc
      421 acgtgtttcc ctcaagcgtc atatctggct cagggatgcc gggcagtgct gtctctgtgg
      481 tcgtgtggtt gacctctgtg acagtgaact cgatcaccga attgcacttc agttcggtgg
      541 tggtaatgag gagacgaatc tctggacgct ctgtaccgaa tgccatcgcc agaagtcagt
      601 cagtgaaacg gcgagtggta tgccggaccc gacgctgccg gaggtgtccg gaggtcatgg
      661 cagggcagac gatatcatcg gactgtgacc cgccccgggg ggggggatca tccggcgaaa
      721 aaaacgatcg ccccggacac cgccccccgt ctcacgcaga gaaaaaattc ccgtttcagg
      781 ccagttaaca tgttaactgg ctgtccgggc atttttgcgg tttttatctt tattattcag
      841 tttgttgcgt ggaaaaaatg ttaacaggct ttttcagcaa atgttaacca ggcagcagtt
      901 aacatttgcg gcatgagacg ccgggaaaaa tgggctgaac catacccggc tgagtgcgtt
      961 atggacccgg gaggaggctg tgctgacaac gcaaaaacga aaatttgcgc tggcgctcat
     1021 gtccgggaaa aacaaaacag cgtcagccat tgccgctggt tattcggcga agaccgccag
     1081 ggttaaaggc tcgcagctgg caaaagatcc ggaggtgctt gcgtttatag cccgtaaaca
     1141 atgcgagacg gtggaggtgg atgaggttcc tgtttaccgg cagaaaaaat cagagccgga
     1201 ggataaaccc cgtcgccgtg aggcggctgc aataccacag ccggacgaaa caaatccgga
     1261 gatgccaccg cccgcggtga tgtctcctgg tattgaatat atggaggacg gtcttcccga
     1321 tccggtgaaa gcgatggggc gtcttctggt ggagaacatt aataccgacc ccaggctggc
     1381 gctggatgcg gcttataagc tggcgcagtt cacgcacca
//
LOCUS       DAZGOB010000346         1417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGOB010000346 DAZGOB010000000
VERSION     DAZGOB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.349"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..292
                     /gene="tnpB"
                     /locus_tag="ACHLIF_005363"
     CDS             <1..292
                     /gene="tnpB"
                     /locus_tag="ACHLIF_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9609149.1"
                     /translation="RNGFNGLASKVQNVLKDDPFSGHLFIFRGRRGDQIKVLWADSDG
                     LCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINWKHPKRTERAGIRI"
     gene            312..>1417
                     /locus_tag="ACHLIF_005364"
     CDS             312..>1417
                     /locus_tag="ACHLIF_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFO9609150.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIG"
BASE COUNT          330 a          405 c          405 g          277 t
ORIGIN      
        1 gcgaaatggc tttaacggcc tggcatcaaa agttcagaac gtcctgaagg atgacccgtt
       61 ctccggacac ctgttcatct tccgcggacg ccggggtgac cagataaaag tgttgtgggc
      121 tgacagtgac ggactgtgcc tcttcaccaa acgcctggag cggggccgct tcgtctggcc
      181 agtcacccgt gacggcaagg tgcaccttac tccggctcag ttatccatgc ttcttgaagg
      241 tatcaactgg aagcacccga aacgaacgga acgcgctgga atccgcatat aacccgttgt
      301 aaagtgagga tatggacacc tcacttgctc atgagaacgc ccgcctgcgg gcactgttgc
      361 agacgcaaca ggacaccatc cgccagatgg ctgaatacaa ccgcctgctc tcacagcggg
      421 tggcggctta tgcttccgaa atcaaccggc tgaaggcgct ggttgcgaaa ctgcaacgta
      481 tgcagttcgg taaaagctca gaaaaacttc gtgcaaaaac cgaacggcag atacaggaag
      541 cacaggagcg aatcagcgca cttcaggaag aaatggcgga aacgctgggt gagcaatatg
      601 acccggtact gccatccgcc ctgcgccagt cttcagcccg taaaccgtta ccggcctcac
      661 ttccccgtga aacccgggtt atccggccgg aagaggaatg ctgtcctgcc tgtggtggtg
      721 aactcagttc tctgggatgt gatgtgtcag agcaactgga gcttatcagc agcgccttta
      781 aggttatcga aacacaacgt ccgaaacagg cctgttgccg gtgcgaccat atcgtgcagg
      841 caccagtacc ttcaaaaccc attgcacgca gttatgccgg agcggggctt ctggcccatg
      901 ttgtcaccgg gaaatatgca gaccatctgc cgttataccg ccagtcagaa atataccgtc
      961 gtcagggagt ggagctgagc cgtgccacac tggggcgctg gacaggtgct gttgctgaac
     1021 tgctggagcc gctgtatgac gtcctgcgcc agtatgtgct gatgcccggt aaagtccatg
     1081 ctgatgatat ccccgtcccg gtccaggagc cgggcagcgg taaaacccgg acagcccggc
     1141 tgtgggtcta cgtccgtgat gaccgtaacg ccggttcaca gatgcccccg gcggtctggt
     1201 tcgcgtacag tccggaccgg aaaggtatcc atccacaaaa tcacctggcc ggttacagcg
     1261 gtgtgcttca ggccgatgct tacggtggtt accgggcgtt atacgaatcc ggcagaataa
     1321 cggaagccgc gtgtatggct catgcccgga gaaaaatcca cgatgtgcat gcaagagcgc
     1381 ccacctacat caccacggaa gccctgcagc gtatcgg
//
LOCUS       DAZGOB010000347         1383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGOB010000347 DAZGOB010000000
VERSION     DAZGOB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.350"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1236)
                     /locus_tag="ACHLIF_005365"
                     /pseudo
     CDS             complement(1..1236)
                     /locus_tag="ACHLIF_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            1213..1383
                     /locus_tag="ACHLIF_005366"
                     /pseudo
     CDS             1213..1383
                     /locus_tag="ACHLIF_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          313 a          396 c          366 g          308 t
ORIGIN      
        1 acggacccga tggcgtgttg ccgccgggcg agggtttgat ttccggtgcc ttaccactga
       61 tgaaggctga ggtgcgcccg gctgcgttca gaatagcggt tgccagacga tccggaataa
      121 tgctcctgcg cgcccatgaa ctgaaatgtg tcgggcggtt tgatgatacc tggtttccat
      181 tcgttctcga tgccgcaccg taatatcctg atgccggaat atccggatct tctgccggcg
      241 cgttagtggc ggtattgacg ccgttaccgt ctgtcatgaa gggcacaaaa taaacgccct
      301 cactctccct gtttttatac ccgccgtaca cggtgtcgta ctgggtagcg tatgtatttt
      361 tccagtaata cgtcgtgtca ccacaaaccc acggcacatc tgcagcactg ccaccatggc
      421 actgcgcgtt aaacacggag aggtcagcac gaaactgtgt cagcatggct gtaaacagcg
      481 caggttgctg tacgtgggtg gcggcgctca tgtcaaactc accctgcatc cagcagacgg
      541 ccagcagaac gtttttggga tttttctgca atgccgcttt tgtgcgggaa atcagatcct
      601 gatataacgg cttgcccacc ccccagcgtg ccgaatcctg actggccccc gtggactcgc
      661 tgaatgtccc ctccgcgccc tgggtaaatg ccgaaccacc acgacagcat ggtaccagca
      721 ggatccccgc gttattcggg atatacggga gcagtttttt ggcaatatgt aaaccctggc
      781 cgacacagcc gtactgccct ttgctcaggt cagccctcgg atgattcagc gtactcatat
      841 cctgcacatc atgcagacag tggtcagccg gaataatatc gttatatctg caggcagccc
      901 cgcccggcgt cactgtactg cggcgcgcca gctgtttaat gcgcggatcc ggagcatcgt
      961 atgaatccgg cagcggaagc ccttcaccgt aagcccttca ccgtaagcca tggcattgga
     1021 ttgcccggcc agtacgatga cgtagtacca atccggctca gatgaagggc cgacctgtgg
     1081 ctctccttca atagccaccg cctgcatcag tgtgtacggc gtaatggcaa ccggtccgcc
     1141 gtatggctgc cagccctctt tcagtttgtg tgtcagcttt tccgcaaggt ctgacggcga
     1201 cgccgccctg acaacatcgt aatgtttaat cgacatcgaa tttctcccgt gtagaggaac
     1261 agagttaaaa agccggaagc ggaatcaaat cacaggatga ccatctgcca gtggcaggtc
     1321 ataaaaaaaa ggctgcgcaa tgcgcagcca gaactcacaa ggaaaatgat aaaaggaata
     1381 aca
//
LOCUS       DAZGOB010000348         1372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGOB010000348 DAZGOB010000000
VERSION     DAZGOB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.351"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..298)
                     /locus_tag="ACHLIF_005367"
     CDS             complement(<1..298)
                     /locus_tag="ACHLIF_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609151.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHI"
     gene            complement(560..>1372)
                     /locus_tag="ACHLIF_005368"
     CDS             complement(560..>1372)
                     /locus_tag="ACHLIF_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO9609152.1"
                     /translation="KVLYELLNTRDMILNELHKHVFLKDDAITPCIFLGDHTGDRFST
                     IFGDKYILTLLNSMRNMESNKDSRINKNVVVLAGNHEINFNGNYTARLANHKLSAGDT
                     YNLIKTLDVCHYDSERKVLTSHHGIIRDEENACYCLGALQVPFKQMKNPTDPEELANI
                     FNKKHKEHMNDPLLHLIRSNIMKPTAVYADYINNTTDFRPKPERIFICGETLKCEDPS
                     IYIRQKYGHHGVGVDQNQQFDNGVMGLNSTKKVLDKNNKIIYSSGLSCFQSH"
BASE COUNT          418 a          256 c          219 g          479 t
ORIGIN      
        1 gaatatgagc gcgcatgccc gatagcatac tatatttttc tgaatgattc gtttgtatgg
       61 taaatgaaat tttgtcatct cgaatagatt gttggaaacg ttctatttgc caatcatcaa
      121 ttttttccac aacttcaccg agcattgtcc tggcaatact tgatatatca tcacctatac
      181 catttgcaat atacccaaac agttctttag tgaagttagt aacatgctct tgcaccatat
      241 catttgttgc gattgtcgtt gtactgtcgt tagtgcctgc cacagttcca attttcatct
      301 tttacctcat ataattcacc gcgaggaatg catagttaac atttaatctt atgacataca
      361 cctcatgttc gatggaaaaa taattatatt ttgcacaaat cattcagcct taaagcataa
      421 tatgaaattt cttatctcat agcagagaaa gtccctaaag attgtagagg cgtcatcagt
      481 acatacctct acaatcgatt aagaaaacct atgaaatact ggcaggatta aaaaggcata
      541 ccaccgtatt catggagttt taatgagatt gaaaacaact aagccctgag gaataaatta
      601 ttttgttatt tttatcaagt actttcttcg tggaatttag ccccataaca ccattatcaa
      661 attgttgatt ctgatctacc ccgaccccat gatggccata tttttgtcta atgtatatgc
      721 tgggatcttc acatttgagt gtctctccac atatgaagat acgttcaggt tttggtctga
      781 aatcagtagt gttattaata taatcagcat atacagctgt gggtttcata atattgcttc
      841 ggattagatg taatagagga tcattcatat gttctttatg ttttttatta aatatatttg
      901 ctaattcctc aggatcagta ggatttttca tctgtttaaa tggaacctgc aaagcaccga
      961 ggcaataaca tgcattttct tcatctctta ttattccgtg atgactggtt aaaactttcc
     1021 tttctgagtc ataatgacaa acatcgagcg ttttaattaa gttatacgta tcaccggcag
     1081 agagtttatg atttgctagt cgtgcagtat agttaccatt aaaattgatt tcatgatttc
     1141 cagccagaac gacgacattt ttattaatac ggctatcttt attactctcc atatttcgca
     1201 tagaatttag caaagtaagg atatatttat caccaaaaat agtactaaat ctatcgcctg
     1261 tatgatcccc aaggaagata caaggggtta tcgcatcgtc ctttaaaaaa acatgtttgt
     1321 gcaactcatt caatatcatg tctctcgtat ttaataactc gtagagcact tt
//
LOCUS       DAZGOB010000349         1372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGOB010000349 DAZGOB010000000
VERSION     DAZGOB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.352"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1372
                     /locus_tag="ACHLIF_005369"
                     /pseudo
     CDS             1..1372
                     /locus_tag="ACHLIF_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          334 a          324 c          430 g          284 t
ORIGIN      
        1 tttaactgtt tttttcatcc gcttgtgggg ctgccgccgg tgtatgccgc aggactggcc
       61 gccatgcagg ggcatcatat tcaggcaaat tcgacgtatt ttttcagaaa tggcggcagg
      121 ccgtccgggg tgattgagat ccccggcagt ataacggaag aaaatgcgaa aaaactgaag
      181 agcaactggg acagcgggta taccggcgaa aatgcgggga aaacggccat tttgagcaac
      241 ggggcaaaat acaaccccac gacgttttca ccggtggatg cgcagacggt ggaacaactg
      301 aagatgaccg ctgaaattgt ctgttcggtg ttccgtgtcc cggcctacaa gattggcgtt
      361 ggccacccgc cttccagtga caacgtggag gcgctggagc agcagtatta ttcccagtgt
      421 ctgcagacgc tgattgagtc cattgagctg ttactggatg aggcgctgga aacgggggaa
      481 aacgagagca cggagtttga cgtcaccacg ctgctgagaa tggacagcga acggcgcatg
      541 aaaacactgg gtgaatcggt gaaaaatacg cttctcacgc ccaatgaggc ccgtaaacgt
      601 gagaacctgc cgccccttgc cggcggtgat gcactgtatc ttcagcagca gaactacagt
      661 ctggaggcgc tgtcccgtcg tgatgcccgt gaggacccgt tcgcgtcggc cgggaaaaca
      721 gtttcagcac aactgcctga cggcgcatct gacggtaata aggcaatcag tgaaacagag
      781 catgatgcag tgaaagcgat gttcaggggg atactgagaa aatgacggaa cgggaactgt
      841 ccattattcg tgcactgggc gaagaattct ccacggtgct ggcggattta cagcgcacat
      901 ttgaggggaa gatggccgcg caggcacaag cgtttgaaga gaaactggct tccctgtcgg
      961 cggtattaca gaagcatgtg acggtggatg aggtacatcc ggttctgcag gcgatggtgg
     1021 atgacgctgt ggggaccatt ccggtaccgc gtgatggtcg tgattatgat ccggatgtac
     1081 tgcagcaggc ggtgaatgat gcggttgcga atattccggt accggcggac ggcaaaagta
     1141 tcacccccga tgatgtgcgt ccgatgcttg agcagatggt gaaagaggct gtaagccata
     1201 tccctgctcc gcgtgacggt cgtgattatg atccggaagt actgaagcag gcggtgaatg
     1261 atgcggtcgc aaatattccg gtaccggcgg acggcaaaag tatcaccccc gatgatgtgc
     1321 gtccgatgct tgagcagatg gtgaaagagg ctgtaagcca tatccctgct cc
//
LOCUS       DAZGOB010000350         1349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGOB010000350 DAZGOB010000000
VERSION     DAZGOB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.353"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..159
                     /locus_tag="ACHLIF_005370"
     CDS             <1..159
                     /locus_tag="ACHLIF_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9609153.1"
                     /translation="RRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQPVSGGRLG
                     VKIYPIMH"
     gene            174..995
                     /locus_tag="ACHLIF_005371"
     CDS             174..995
                     /locus_tag="ACHLIF_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9609154.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            1139..1214
                     /locus_tag="ACHLIF_005372"
     tRNA            1139..1214
                     /locus_tag="ACHLIF_005372"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1172..1174,aa:Ile,seq:cat)
BASE COUNT          356 a          276 c          395 g          322 t
ORIGIN      
        1 cgtcgcgacc tggacaacat tctgaaagca ccgctggatg cgctgacgca tgcgggagtg
       61 ttaatggacg atgagcagtt tgatgaaatc aatatcgttc gtggtcagcc agtatctggt
      121 ggacgtctgg gggtgaagat ttaccccata atgcattaag agcaggtcaa aaaatgaaac
      181 tggaagattt accgaaatac tactccccaa aatcccctgg cctgaccgat gcatcggcct
      241 caacgtcaaa agatgcgctg agtatcactg atgtgatggc cgcgcagggc atgacacaga
      301 atcgggctga gatgggtttt tctgcgttcc tggggaaaat gggcatcagt atgaatgaca
      361 gggcgcgggc aacagaatta ctggcagatt atgcactcag tcggtgcgat cgtgtggcgg
      421 cgttgagaaa acttccggca gaaataaaac cggtagtgat gcgcattatg gcttcgtacg
      481 cttttgagga ttatgcccgc agcgcagcga gtaaaaagca gtgcccttgt tgctatgggg
      541 aaaaatttat tgaaagcata gtttttacaa acaaggtcca gtatccggat ggtaagccgc
      601 cggtatgggc aaagtgtacg aaaggtgtgt atccgtctta ctgggaagaa tggaaaaaag
      661 tcagggaggt ggtaaaagtt gcctgtccgg agtgtggcgg aaagggtgag gtttccaccg
      721 cctgtaagga ttgccgtggg cgtggtgtcg ccattcatcg tgaagagtcg gtaaaacgtg
      781 gtatgcctgt tatcagagac tgccagcgtt gtggtggtcg tggctatgaa agactaccat
      841 caacggaggc atttaatgct atatgcgagg tgacaaacca gataacacgc gcgtcatggg
      901 aaaaaacagt taagaaattt tatgatgcgc tggtgacccg gtttgatatt gaagaagcat
      961 gggctgagcg gcagttaaaa aaggtaacta ggtaacaagg ttgatttttc cggaatctgt
     1021 ggtaaattcg tcataacgat gggcgtttta tgcctgacgt tagaagagtt tctacaaccc
     1081 gccgctgagc gggtttttta ttgcgaaatt aattacggac cgttattatt ctgctcccgg
     1141 ccctttagct cagtggtgag agcgagcgac tcataatcgc caggtcgctg gttcaaatcc
     1201 agcaagggcc accatcacaa accgccatta gcttatcagg aagagcagac gacacgataa
     1261 cagggttgtt ggtgcggggg gcgggtcccc gatggcggtc cattatcggt attcagcgtt
     1321 gttagctcag ccggacagag caattgcct
//
LOCUS       DAZGOB010000351         1347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGOB010000351 DAZGOB010000000
VERSION     DAZGOB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.354"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..364)
                     /locus_tag="ACHLIF_005373"
     CDS             complement(<1..364)
                     /locus_tag="ACHLIF_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609155.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDIPESPTGNNLEALAPGIEKPKQ
                     TSIEMVTLLNTLQPDGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQKISTG"
     gene            complement(386..925)
                     /gene="yhaB"
                     /locus_tag="ACHLIF_005374"
     CDS             complement(386..925)
                     /gene="yhaB"
                     /locus_tag="ACHLIF_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO9609156.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1181..>1347)
                     /gene="tdcR"
                     /locus_tag="ACHLIF_005375"
     CDS             complement(1181..>1347)
                     /gene="tdcR"
                     /locus_tag="ACHLIF_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO9609157.1"
                     /translation="AARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDR
                     TSFCECNRFP"
BASE COUNT          410 a          233 c          257 g          447 t
ORIGIN      
        1 tgcctgtgga tattttctgg ttctttaatg gttcattaat ggtaagttcg caggtattgt
       61 ccgatcgttg aataatttgg gcgttataac ctaaaaaatc cagacgaaga gacgagtcat
      121 tataaagaat tacattttgt aagtacgcta attctttttg aaaatcacca gtgataatgc
      181 attttccatc aggttgtaac gtgttaagta aagtgaccat ttcaatagag gtctgttttg
      241 gtttttctat acctggtgct agtgcttcga ggttatttcc tgttgggctt tcaggaatgt
      301 cattcatttt tcgacggact aaatcactac taatatcatt accaattgat gaaacaggga
      361 acattatttt atcctttata aataatcaat aaaaatcatt attaaaatga tcgggtgaaa
      421 aaaagagaat ttgccaacaa tctgaagtaa tgctggtttt attaaagtgc acaaggggtg
      481 ttaagatggc ttcttttcgg gtgaattttg cacaatactt agaaatcgtc tccttaatac
      541 attccagctc ttttttattg acggctgagc aagtacattg ttcgccaaaa ggtgcaaaag
      601 tgcctacatc taatgtttgt atgatataat catcatgttc ccctgtagaa atatctatga
      661 tgaatggact catattttcc ggttggattc ggatcattac agaaggaaca tcgccttgat
      721 agaaattcaa tgcagtaatt gcagataata ctctccgcag ttcattactt atactgtaaa
      781 ttttattaac gtcattggtg gagacaattt ctgcaaactt tctttttacc gtccaataat
      841 caatatttct attgtgattg ttaaccactg cgtgcattgg cgggattgaa gggtgaaaaa
      901 tatgtgcaat tgggaagcct ttcattgatg cctgccttgt cgtcatgaca aacaataata
      961 gatgcttatt ttcctcgcag ggttctttcg cactatcaaa aaacgctcat ttacccggcg
     1021 ttacagtaaa cgaagcaaca ttctgtggcc tacgacgatc aaaagctctg tgaaaatttc
     1081 ccttgttgtc tttgtctgac cttatcgcgg cgaccagcaa ttattcgttt tgtgatcctc
     1141 aacacaaaat gctattcgtc gcctatgagg ggagaagaat ttatggaaaa cgattgcatt
     1201 cacaaaatga cgtcctgtca gaagagaaaa agttgacgtt aatcaattgt tcgagatgat
     1261 caatacctgt attcgaaatt ttaaaatatt gataaaataa atgtttgttc tccaacaaat
     1321 agcgtgaaaa aatgtgacgt gccgcag
//
LOCUS       DAZGOB010000352         1345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGOB010000352 DAZGOB010000000
VERSION     DAZGOB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.355"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1345
                     /locus_tag="ACHLIF_005376"
     CDS             <1..>1345
                     /locus_tag="ACHLIF_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO9609158.1"
                     /translation="VMANWDDESSTLSVYSIDMIAWRDKNKTEKEPLPLDVIQLTNWR
                     IHNSCSLFYDKYQVDIEANKLTYTILFPDTELPVQLNYASVIYGNHGAKYTFLDSGSK
                     AITIHITDESGDYDTFDFRNIVFEHHTNDMFISFDNQGGFVISISNNATNELTDINVF
                     RKNMTSFDGCRSLIYLPSGDVFYISDIYKMSRGRKSVKLNVEKKPDINDIINVAILET
                     SYLQIKKIPNNDDGSYVLCLDNPNLSSYTLHFNDLSGYISSIWDNIRGSFTPFHNNTV
                     NIAPNEKKFISLTEPDKLSFNIDVFREALEVKNSYKLSKFTWETYGDIVVSPEDRISH
                     LELDGFNYFSQPELDAQISDTFSYLYDNFQIADGDVHIKLLHLDRETKQITPHRIILQ
                     KYFIDSFAKTSISDRENNIYPVICDSPDHFTNDIYRHPFRIVLGNKTLYPSEELI"
BASE COUNT          476 a          211 c          214 g          444 t
ORIGIN      
        1 gagttatggc aaactgggat gatgaatcaa gtactctgtc tgtttattca atagatatga
       61 ttgcctggag ggataaaaat aaaactgaaa aagagccttt accacttgat gtgattcagt
      121 taactaattg gaggatacat aactcatgct ctttatttta tgataaatat caagttgaca
      181 ttgaagccaa taaattaacg tatacaattt tatttcctga tacggagtta cctgttcaac
      241 ttaattatgc tagtgttatt tatggtaatc atggggcaaa atatacattc ctcgactcag
      301 gaagtaaagc tataaccatc cacattactg atgaaagtgg cgattatgat acttttgact
      361 tccgcaatat tgtttttgaa catcacacca atgacatgtt tataagcttt gataatcaag
      421 gaggatttgt tatatcaata tcaaataatg ccacaaatga actgacagat attaatgtat
      481 tccgaaaaaa catgacttct tttgatggct gtcgttcatt gatatatttg cctagcggtg
      541 atgtttttta tatttcagat atctataaaa tgtccagagg aagaaaatcg gttaaattaa
      601 atgtagaaaa gaaaccggac attaacgaca tcatcaatgt ggctatttta gaaacttctt
      661 atctgcaaat aaaaaaaatc ccaaataatg atgatggcag ttatgtttta tgtctagaca
      721 accctaacct ctcatcttac acattacact ttaatgactt atctggatat atatcaagta
      781 tatgggataa tattagagga agcttcactc cttttcataa taatactgtg aatatcgccc
      841 ctaatgaaaa aaaatttatt tccctaacag agccagataa gttgtcattc aacattgatg
      901 tttttagaga agcattagag gtaaaaaata gttataagtt aagtaaattt acatgggaaa
      961 cgtatggtga tatcgtggta tcacctgaag atagaatttc tcatctggaa ttagatggat
     1021 ttaattattt ttcacaaccc gagttagatg ctcaaatatc agacaccttt tcatatctat
     1081 atgataattt tcagattgct gatggtgatg ttcatattaa gcttcttcat ctcgatagag
     1141 aaacaaagca aataacgcca catcgtatta tccttcaaaa atactttata gactcatttg
     1201 ctaaaacaag tatctctgac agagaaaaca atatatatcc tgttatttgt gattcacctg
     1261 atcacttcac aaatgatata tataggcatc catttaggat agttctcggg aataaaacat
     1321 tgtatccatc agaagaactt ataaa
//
LOCUS       DAZGOB010000353         1344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGOB010000353 DAZGOB010000000
VERSION     DAZGOB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.356"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..448
                     /gene="yqeJ"
                     /locus_tag="ACHLIF_005377"
     CDS             <1..448
                     /gene="yqeJ"
                     /locus_tag="ACHLIF_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO9609159.1"
                     /translation="VFISGFILFTVYSYTAEKMIYNETCTANWVIFNDQGRANLTIDF
                     MYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTSKKVNKFEIMDQV
                     DDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR"
     gene            complement(481..561)
                     /locus_tag="ACHLIF_005378"
     CDS             complement(481..561)
                     /locus_tag="ACHLIF_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609160.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(597..1022)
                     /gene="yqeK"
                     /locus_tag="ACHLIF_005379"
     CDS             complement(597..1022)
                     /gene="yqeK"
                     /locus_tag="ACHLIF_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO9609161.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            1216..>1344
                     /locus_tag="ACHLIF_005380"
     CDS             1216..>1344
                     /locus_tag="ACHLIF_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609162.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLN"
BASE COUNT          489 a          229 c          213 g          413 t
ORIGIN      
        1 agtttttatc agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat
       61 atataacgaa acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct
      121 taccatcgac tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac
      181 ctggcaacaa ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat
      241 tgaaaactat gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga
      301 tcaagttgat gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa
      361 aagtgtaagc tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa
      421 cagagcaata atgcattgtg caaggtaaac taaaatagat aacaaaaaga tggaacctcg
      481 ttaatcataa aacaataaaa ttaaatattc tcgcagtata tggcagtcta aagcatcaaa
      541 gttttgatca acatctttca ttttagacat ctccttgcaa tgtaaaatat catgtatcaa
      601 caacatctgg tgcaaatcca tgagtcgaac tcgacattca tcttagctcg attattattc
      661 cttcgtacag tcgatgtaaa caatacagaa agaggattat taagaaccag tttactcagt
      721 gaatcaatag aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa
      781 ctaaactcat cattgacaga tcgtgagata taactgcttt taactttact ctttacgttg
      841 actttattga cagaattaac attcacaaat cttgaattta atgcccatga tgttgtttgc
      901 gtgagaacct tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag
      961 tccgaattta caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc
     1021 attatatacc tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc
     1081 aatactaaaa ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaatgtc
     1141 tccataaggt gatatttgac attatcagaa gctgcgaatt cggattttgc tctaatcaaa
     1201 tgagagaaat gtaaaatgaa gccacgaaat attaataata gcctaccact gcaaccatta
     1261 gttcctgatc aggagaacaa aaataagaaa aatgaagaga aatccgttaa tccagttaaa
     1321 atcacaatgg ggtctggttt aaat
//
LOCUS       DAZGOB010000354         1343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGOB010000354 DAZGOB010000000
VERSION     DAZGOB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.357"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(142..486)
                     /locus_tag="ACHLIF_005381"
     CDS             complement(142..486)
                     /locus_tag="ACHLIF_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609163.1"
                     /translation="MSDLSMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEKIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(515..682)
                     /locus_tag="ACHLIF_005382"
     CDS             complement(515..682)
                     /locus_tag="ACHLIF_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609164.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(755..1039)
                     /locus_tag="ACHLIF_005383"
     CDS             complement(755..1039)
                     /locus_tag="ACHLIF_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO9609165.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            complement(1032..1175)
                     /locus_tag="ACHLIF_005384"
     CDS             complement(1032..1175)
                     /locus_tag="ACHLIF_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609166.1"
                     /translation="MKVWVYDPETILNVEERRRTEVYIHNLNEDGFAPVLVKATPQEV
                     NRG"
     gene            complement(1185..1334)
                     /locus_tag="ACHLIF_005385"
                     /pseudo
     CDS             complement(1185..1334)
                     /locus_tag="ACHLIF_005385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
BASE COUNT          331 a          325 c          280 g          407 t
ORIGIN      
        1 tggcgtgcat tccactcctg aagtgtcaag tacatcgcaa agtctccgca attacacgca
       61 agaaaagccg cattgatgcg gcgatggtag gtctggatat cattgagcca tgaacaggcc
      121 tcatcgagtg tgaggctgtg gttagtcctt tcgtaactcg ctgattctcc tgtaagtctc
      181 tggtgctttg tttccgtgta tcttcatttc agacttcaac agagcaacga gggaatccca
      241 ttcgttgagg atgcctttga atgccggaac gcgctttgca accttgtcga atgaatctct
      301 gatttctgga atcttctcaa caagtgcaac gcatcgtcgg aagtctgctg cgtcatgggg
      361 agcgccgaag tgatgaccat agatattctt tttcaggcca catgcgattg aggcaagagt
      421 tgcgctactg atgccaacat cgccagtcga ttgccatttc aaaaccttca tagacaaatc
      481 tgacatttct tgtctccaat aaaaaaaacc tccatcaggc ggcttggtgt tctttcagtt
      541 cttcaattcg aatattggtt acgtctgcat gtgctatctg cgcccatatc atccagtggt
      601 catagcagtc attgatgttc tctgcttcga taactctgtt gaatggttct ccattccatt
      661 cacctgtgac tcggaagtgc atttatcatc tccataaaac aaaatccgcc gtagcgagtt
      721 cagataaaag aaatccccgc gagtgcgagg attgttattc attgccgata ttcaccttta
      781 tcgcgaacac ctttaccggt ttatcaccga agtgcggatg tgtgattgtc ttgatttcat
      841 atccgtcata cggaacatca attctgcggc tggaatcgtc gcgcttcgga tatccctttg
      901 tgataatcag gcggtcatac tcccggaaca taattcgctt attccaatag tcattacaca
      961 ggcgatactc ttccgttttc tctcctcgaa tcatggcatc gaagtattca cctttgacgg
     1021 caagttgtag gttagccacg gttaacctcc tgcggagtag cctttacaag cactggtgca
     1081 aatccatctt cattaaggtt atgaatatag acttctgttc tcctgcgctc ttcaacgttt
     1141 aatattgttt ctggatcata aacccatact ttcattcgac tattccatga agaaatcgct
     1201 tcagatctag ttcgtttttc tggtccttgg gcaccacatt tgcatgacac atagcgcatt
     1261 ttccctctga tactgaatga gtatccgatg ttaagcacag tggaaccaca gaatgggcag
     1321 cgatatagat tcataaatca tcc
//
LOCUS       DAZGOB010000355         1342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGOB010000355 DAZGOB010000000
VERSION     DAZGOB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.358"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..298)
                     /locus_tag="ACHLIF_005386"
     CDS             complement(<1..298)
                     /locus_tag="ACHLIF_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9609167.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTSAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSV"
     gene            complement(366..>1342)
                     /locus_tag="ACHLIF_005387"
     CDS             complement(366..>1342)
                     /locus_tag="ACHLIF_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9609168.1"
                     /translation="NSQLADEMAEMKTSITETRNEITQTVSKTLEDQSATIQQIQRVQ
                     KDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESG
                     NSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPAFSLTPDGRLTAKNADISGSVNA
                     NSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKF
                     DRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSV
                     IDMPAGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
BASE COUNT          285 a          366 c          327 g          364 t
ORIGIN      
        1 tcacgctgaa ccagcggtta cgcacggaat cttcatgcca gcgggtatcg ctgtaacggg
       61 taagctggcg attcctgtct cctgcatagc tgaatgacgt caccagcccc agtgtgtccg
      121 taaactcata acggtatttc acgttaatcc cgttaagatt atcgctaccg ggagcgctcg
      181 tccgggcatg aagatacccc gcgctcagcg tggcctgctg ctcagacgcc catgcaggcg
      241 cgccggatac ggccagacag atggctgcgg acaaaatggc ggcataaagt ttacgcataa
      301 ttacctctcg cttttctgca ataaaaaaag gcgtcatttc tgacgcccgt tctgggttat
      361 aaaattcagc tgatactgat acctgctgtg gattttttca tcaccacaac cagcagatcg
      421 ctgatactgg ttgttggtgt ccagttattc gctcctgatg aagatacggt gaatgtcagt
      481 gtcagcgtcc cctgtccggc aggcatatct ataactgagg aaaatacgcc ctgagcatcc
      541 gtcgtggact gattaaaaat ctcctgacca ttgcgggtca ctcttaaccg gcaggttgaa
      601 taccagtatg actgttggtt attactgttg aaattctcat gcttaccacc gcggaataac
      661 actggcggta tcatgacctg ccggtcaaac ttctgatcat cactgattct taccgtgatg
      721 gtgccactgg cataagtgct cgtgcggggg aaagacttgc tgaccgtttt gacaatatcg
      781 ccttcaatct ggttggctga cagtttcccc ttaatctgac agttctcatt aatcgtgacg
      841 ttgttgagcg tccctgagtt cgcattcaca ctgccactga tatccgcatt tttcgccgtc
      901 agtcgcccgt ccggcgtcag ggaaaatgcc ggaggattgc cggatgacgt gatgctcacc
      961 gcaaacagtc gtttcaggaa cacgtcgttc atgaacagct gattcccctg cgccacaaat
     1021 aacggcgtgc tgttgccgct ctccggattt atcatcgcga tacggtcagc cagcagcagt
     1081 atgttgctca gtggctggcc atcagtatcc tcaatccctg caccaatccc ggccacatag
     1141 ggaatgccgt ctttcgtttt ttgaaccttc agcatgtaca gcgcagccag gtcatcattt
     1201 gtgtccttct gcacgcgctg tatctgctgt atggtggcgc tctggtcctc cagcgtttta
     1261 ctgaccgtct gtgtgatttc attgcgggtt tccgtgatgc tggtcttcat ctccgccatc
     1321 tcatccgcaa gctggctgtt at
//
LOCUS       DAZGOB010000356         1326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGOB010000356 DAZGOB010000000
VERSION     DAZGOB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.359"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            55..279
                     /locus_tag="ACHLIF_005388"
     CDS             55..279
                     /locus_tag="ACHLIF_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9609169.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLINEKYSQFVPNKDKHDQSTCSDRFT"
     gene            complement(361..675)
                     /locus_tag="ACHLIF_005389"
     CDS             complement(361..675)
                     /locus_tag="ACHLIF_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9609170.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
     gene            complement(1141..1275)
                     /locus_tag="ACHLIF_005390"
     CDS             complement(1141..1275)
                     /locus_tag="ACHLIF_005390"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO9609171.1"
                     /translation="MDNATGPRLADTAERDYFILRERLMAMQKQLEGAQEYIRTQCIP
                     "
BASE COUNT          397 a          334 c          221 g          374 t
ORIGIN      
        1 gtaaaaacaa cttcagacaa tacaaatcag cgacactgaa tacggggcaa cctcatgtca
       61 acgaagaaca gaacccgcag aacaacaacc cgcaacatcc gctttcctaa ccaaatgatt
      121 gaacaaatta acatcgctct tgacctgaaa ggttcaggta atttttcagc gtgggttatt
      181 gaagcctgca gaagaagatt aattaatgaa aaatattctc aatttgtacc caacaaagac
      241 aaacacgacc agagcacctg ttcagacagg tttacttaaa cgacttatat atgacacaaa
      301 aagcgaccac taaagtcgct ttttcttatg gtaacaggca ataactctct cagatatttt
      361 ttagcatttt tttgaccgcg cgtttccgga cgtattctgt tctcctgtcc ctttatatcg
      421 tcggaatacc cgccgctctt caaatcccat tcccaactca gaatgtagtc tgttgaccgc
      481 ttgttttatt tcggtcaggt tcaccggtga aaccggagtc cggcgcgcct tacgcaaaca
      541 ctctgctcgt ttctgtgccg ccacttttct tttctggtca tcacttagct gtaccatcac
      601 ttttgcccat cgttcagctg ctctccggta cagtcctttt ttctccagac attctgccac
      661 gtgatcatgt agcataagtg acctccgatt atctacagac tgccatcctg aatttacctt
      721 cccttaatga aataacaata aaaaacaaac cacgcaaaaa caataaaaca acacacaaaa
      781 aaaactaaat aataaacaaa aataatcacc ttattttatt attttttgag ggagcaatta
      841 ctgaacaaaa aacgctgact atatactcaa aaccaaacaa ctattctgcc aatcaggtat
      901 catggcaaca cacggaatta ccgtgttttt gccttctctg cccatacaat acgggcatat
      961 acttcattct ctattgtaat atttctatcc atgtgcccca ctccatttac ctgtaaataa
     1021 tattcaaaat atttatcaca gaaatcgttt ttggccatga actgagcaca ctataaagtc
     1081 cggaactgac tctttgttaa attaccttaa cgttaccagt aacaccttca taacaaaata
     1141 tcacggtata cactgggtac ggatatattc ctgtgctcct tccagttgct tctgcattgc
     1201 catcagccgt tctctgagga tgaaataatc ccgttcagcg gtgtctgcca gtcgggggcc
     1261 ggttgcatta tccacgccgg aggtgccggt ggcttcacgc acggtaccgg agcaggtggc
     1321 gttgat
//
LOCUS       DAZGOB010000357         1325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGOB010000357 DAZGOB010000000
VERSION     DAZGOB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.360"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            82..405
                     /locus_tag="ACHLIF_005391"
     CDS             82..405
                     /locus_tag="ACHLIF_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609172.1"
                     /translation="MGRNMSAARTDGFIRNIHSRNPFDVIRADVVISRLEKQAHWGCG
                     LHYEIYEANLFDMAMNHLSRLPLKDRPVFIGAAARKGLFLTMEETRLAREAKDALMAE
                     LALEY"
     gene            464..706
                     /locus_tag="ACHLIF_005392"
     CDS             464..706
                     /locus_tag="ACHLIF_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609173.1"
                     /translation="MGYITYVTDQRPGEPDILTGNTFADLEIRDSDGHLLLKVSAPEA
                     GWTHESLNLVQPQEVQEGNDAFDAYLNGIWIGSTEV"
     gene            complement(907..1325)
                     /locus_tag="ACHLIF_005393"
                     /pseudo
     CDS             complement(907..1325)
                     /locus_tag="ACHLIF_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          277 a          335 c          381 g          332 t
ORIGIN      
        1 agcaggtccc cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacagat gatgggggca
       61 aaaaacagag gtgtattaaa gatgggcagg aatatgtcag cagcacgtac cgatggcttt
      121 attcgtaata ttcattcccg caatcccttt gatgttatcc gggctgatgt ggtgatcagc
      181 agactggaaa agcaggctca ttggggatgt ggtctgcact atgaaattta tgaagcgaat
      241 ttgtttgata tggcgatgaa tcatctttcc cggcttcctc tgaaggatcg tccggtattc
      301 attggtgctg ctgcacgaaa ggggcttttt ctgacaatgg aagaaaccag gctggcacgg
      361 gaagcgaaag atgctctgat ggctgaactt gctctggaat actgaaaatc gggccgcacc
      421 gtcgtcccgg atagtgctga aatatttaat gtggagagaa tggatgggat atatcacata
      481 tgttacagat caacgacccg gtgaaccaga tatcctgacc gggaacacat ttgctgatct
      541 ggagattcgt gattctgatg ggcatcttct gctgaaagtg tctgcacctg aagccggctg
      601 gacacacgaa agcctgaacc ttgttcagcc gcaggaagtg caggagggga atgatgcatt
      661 tgatgcttat ctgaatggta tatggatcgg ttcgacagag gtctgaacaa atcacgccag
      721 cagaagacgt tgctggcgtc ttctgcattc ccgggggagc tgtcgcgcgg catgtttgct
      781 tatacccgtg ccccgagatg attattcgtc tgcgggcctg aagacgccgg gcaggcggga
      841 ggataaatgc ctgtctgccc gcaccgggaa aagaccgcac gccggtggcg ctgaaagggg
      901 ccgcgctcac gcgccgcccc tttcattacg cggatgttct gctgtgtgct tctgtctttt
      961 catgcctgtc atttgtctgt tactgatgat gttgttgttg ttcctgctac tgttcttctt
     1021 ttttctgctg ctctgattac tctcagtttt gttgttctcc tggcagtgcc ttttccgggc
     1081 gtccccctgg cggggcgggc tgtcgtgaac ggagccagcg gagctgtctc cgccctggcg
     1141 ggcttccatc cctgacgcaa aaacccgggt ttgcagcgac cggaacggtc gcgccaccct
     1201 tgtcgcgccg tgacctgcgc tcctggcatc ccggcaaaaa gacgccggga tgtaaggagc
     1261 aacgccgttt cgcaaaccgc gcttcacgtc gttgcggcgg atccctgccc gttgttctgt
     1321 ccggt
//
LOCUS       DAZGOB010000358         1325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGOB010000358 DAZGOB010000000
VERSION     DAZGOB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.361"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..212
                     /locus_tag="ACHLIF_005394"
     CDS             <1..212
                     /locus_tag="ACHLIF_005394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609174.1"
                     /translation="ADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILA
                     GISAGGMYCPECGTVHWPDGVPPPF"
     gene            212..>1325
                     /locus_tag="ACHLIF_005395"
     CDS             212..>1325
                     /locus_tag="ACHLIF_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFO9609175.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRRLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQEWHPHI
                     HLSTTTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGGQMRFTG"
BASE COUNT          296 a          318 c          412 g          299 t
ORIGIN      
        1 gcgcagacct gaatattccc ttctttgtgg atgccggaaa caaaaagctg gtcttctggt
       61 ttgtacgtgt tgatgacgaa gggtatcctg aaatagcccg ctgcacggag cgggagtttg
      121 cgaccattct tgccggtatc agcgccggcg gcatgtactg cccggagtgt ggcacggttc
      181 actggccgga cggagtcccc ccgcccttct gatgcttccc cgttttgccg acatttttca
      241 gcagggaaac cgctggctta actggctgga gaaacaaccg gaaggttcag tgcgtccggt
      301 agtcattgag tctgtgacaa aaatcatggc ctgcgggacc acgctgatgg ggtacacaca
      361 gtggtgctgt tcatctccgg actgcagcca cataaaaaag gtctgcttcc ggtgtaaaag
      421 tcgctcctgc ccgcactgcg gagtgaaggc tggcgcacag tggatacagt atctgctgag
      481 tctggttccc gactgtccgt ggcagcatat tgtgttcaca cttccctgcc agtactggtc
      541 cctggtgttc cacaaccgga ggttactggc agagatgagc cgcattgctg cggatgtgat
      601 acaggaaatc tgccgccagg cagatgtggt gccggggata ttcacggtga tccacacatg
      661 gggacgtgac caggagtggc atccgcacat tcacctgtcg acaacgaccg gcggcgtgac
      721 atcagaccac acctggaaaa accttcattt ttacgcccgt aaggtgatga gtatgtggcg
      781 ttaccggata acgcggttac tgtcacggaa atatccggac ctggtgatac cggatgcgct
      841 ggcagcagaa ggaagcagta aacgggactg gaatcgcttc ctggacagtc attaccggcg
      901 gggctggaat gtcaacgtat cccgggtgat ggataacgcc acacatgtgg cggtgtactt
      961 cggctcttac ctgaaaaaac cgccggtgcc gatgagccgt ctggagcact atgctggtca
     1021 ggatgaaatt ggtctgcgtt acaacagtca ccggacaaaa cgggaagaat acctggtgat
     1081 gagtggtgat gagtttatgg aaaggttctc ctggcatgtg gcggataagg ggttccgtat
     1141 ggtgaggtac tacggtttcc tgagtccggt gaagcgccgg ttactggaag atgttgtgta
     1201 cgtcataacg gagacggtga gaaagacggc gatgcaaatc aggtggagag ggatgtatca
     1261 gcggttactg aaggttgacc cgctgaagtg catcctgtgc ggaggtcaga tgcgttttac
     1321 ggggc
//
LOCUS       DAZGOB010000359         1307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGOB010000359 DAZGOB010000000
VERSION     DAZGOB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.362"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHLIF_005396"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHLIF_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO9609176.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          387 a          244 c          270 g          406 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaa
//
LOCUS       DAZGOB010000360         1302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGOB010000360 DAZGOB010000000
VERSION     DAZGOB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.363"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..281)
                     /locus_tag="ACHLIF_005397"
     CDS             complement(<1..281)
                     /locus_tag="ACHLIF_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO9609177.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAAS"
     gene            complement(291..1169)
                     /locus_tag="ACHLIF_005398"
                     /pseudo
     CDS             complement(291..1169)
                     /locus_tag="ACHLIF_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
     gene            1210..>1302
                     /locus_tag="ACHLIF_005399"
     CDS             1210..>1302
                     /locus_tag="ACHLIF_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9609178.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPR"
BASE COUNT          243 a          394 c          364 g          301 t
ORIGIN      
        1 tcgctggcag cctccggcca gagcacatcc tcataacgga acgtgccgga cttgtagaac
       61 gtcagcgtgg tgctggtctg gtcagcagca accgccagaa tgccaacggc agcaccgtcg
      121 gtggtgccat cccacgcaac cagcttacgg gtggcggtgt ccagcatcag cggggtcatt
      181 gcaggcgctt tcgcactcaa tccgccgggc gcggttgcgg tatgagccgg gtcactgttg
      241 cccagcggct ggtaatgggt aaaggtttct ttgctcgtca taaacatccc ttacactggt
      301 gtgttcagca aatcgttaac ggcatcagat gccgggttac ctgcagccag cggtgccggg
      361 gcaccctgca tcagacgatc cagcgcagtg tcactgcgcg cctgtgcact ctgtggtgct
      421 gcggccagaa tgcggcgggc cgtttccacg gtcataccgg gggtttctgc cagcacgcgt
      481 gcctgttctt cgcgtccgtg agcctcctca cagttgagga tccccataat gcggctgttt
      541 tctgccgcaa ccgctgcggt gatctgcgcg ttcacgtccg gctgcgccgc gctggcgttt
      601 tcgccctccg tcgctggcac cacgtcagta acgtcagcct gcgaagcagt ggctgaaaca
      661 gttgttgatt gagtctcttt ggtcattcgc cctcctgaga gacgggattt acgtgcatcc
      721 agtgcatcac gcatgacggt gatcgcatcg gtgctgttaa caagttcatc agccagtccg
      781 gcatcaatgg cctcctgacc gctgtacact gcagcctcgg tatccagcac agcctgcacg
      841 gacaggccgg tatatgccga caccttctgc gcaaacatct ggcgggttgc gtccatccgg
      901 tactgcagtg tctcccggac gtcatccgga agatggctgt aggggttgcc atccacctta
      961 tggctgccgc tgtaaatcag cgtgatttcc acaccctgtt tctccagcgc agcaccgtaa
     1021 ttactgtgag ccatcatgac gccgatggag cctgtccggg cggtctgcgt gaccagacgc
     1081 cgggaggcgg cactggcaag caactgacct gcactgcagt tcatgtcgtt ggcaagcgcc
     1141 cataccggtt ttatgtgtaa ccggctcatt taaaccgtct ggtctgtttc ctccggctct
     1201 acaaaaataa tgtccatcat ttttaatgga cactatcgta tgaaacaccg gacctggatc
     1261 actgaagctt tacgtcttca ctttgaagaa catttacccc gg
//
LOCUS       DAZGOB010000361         1293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGOB010000361 DAZGOB010000000
VERSION     DAZGOB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.364"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..377
                     /gene="stpA"
                     /locus_tag="ACHLIF_005400"
     CDS             3..377
                     /gene="stpA"
                     /locus_tag="ACHLIF_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO9609179.1"
                     /translation="MRTLRAMAREFSIDVLEEMLEKFRVVTKERREEEEQQQRELAER
                     QEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDVNGETKTWTGQ
                     GRTPKPIAQALAEGKSLDDFLI"
     gene            complement(424..948)
                     /gene="ygaP"
                     /locus_tag="ACHLIF_005401"
     CDS             complement(424..948)
                     /gene="ygaP"
                     /locus_tag="ACHLIF_005401"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO9609180.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(958..1257)
                     /locus_tag="ACHLIF_005402"
     CDS             complement(958..1257)
                     /locus_tag="ACHLIF_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO9609181.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          342 a          315 c          312 g          324 t
ORIGIN      
        1 acattcgcac tctccgtgcg atggctcgcg aattctccat tgacgttctt gaagaaatgc
       61 tcgaaaaatt cagggttgtc actaaagaaa gacgtgaaga agaagaacag cagcagcgtg
      121 aactggctga gcgtcaggaa aaaattagca cctggctgga gctgatgaag gctgacggaa
      181 ttaacccgga agagttatta ggtaatagct ctgctgctgc accacgcgct ggtaaaaaac
      241 gccagccgcg tccggcgaaa tataaattca ccgatgttaa cggtgaaact aaaacctgga
      301 ccggtcaggg ccgtacaccg aagccaattg ctcaggcgct ggcagaaggt aaatctctcg
      361 acgatttcct gatctaatta ctgaggccgg atgtcgctgc tagggcgcgt ccggcgtcgc
      421 ttctcaagtt cgttggttcc acggcatctt atctaacaac cttgccattc cacaaaaacc
      481 gctgattcct gcaaacagta acccggcacc aacaaagcca cttaataaga agaaaccgct
      541 atttacggta taacccagta caacgccgat taatattaaa ccacccgcag cgatctgcac
      601 ctggcgcatt aacggcaagg gttgagattt atttaccgct actggcaatc cagctctttt
      661 ccagccatca atcccatctt cgagtaaaaa tatttctgcg ggggcggcaa tcgctgctaa
      721 tttatcggcg ttattactgg tgcgtttacc tgcctggcag tggaatataa tttgctcgcg
      781 acgtaattta ggtggaagac ctgactgttc cagcacggat aatggagcca gatctgcttc
      841 aggaatatgt tcacgaagat attcatcagc gtcacgaata tcgattaact ttgcgccgcg
      901 tgcgattaat tcttgtgcat catgcggcga aatggttgtc aaagccatag tgactcctta
      961 cggacaatag acatttttca gggtggcgat aatggcattt accgcctcat ttttaatgga
     1021 atatagaatg cgttgggcat cccgttggct gtcgataagc ccttcgtccc gcatacgagc
     1081 gagatgctgt gatgtcgcag aggcactcag tccggtaatg cgcgtcagct ctcccgcgct
     1141 ggtgccgggg gaaccgctaa gcatgcacag aatcagcaat cgtttaggat ggctcattgc
     1201 tttcaataag gccgctgcct gttcggcact ggcctgtaat tgcgcgagtt cagtcatatt
     1261 tatttaagta ttttctaaat taagtaaact cta
//
LOCUS       DAZGOB010000362         1286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGOB010000362 DAZGOB010000000
VERSION     DAZGOB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.365"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..285
                     /locus_tag="ACHLIF_005403"
     CDS             <1..285
                     /locus_tag="ACHLIF_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_100495227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFO9609182.1"
                     /translation="QSIAEIRSAFIPLSIMSFAIFMGIYNFMFGSVGLSIRGYKKEFS
                     YIVAITGVSTIILSLCLSYFFAEIGAAIAYVFAEFILLILILRIYKVKRL"
     gene            351..>1286
                     /gene="wzy"
                     /locus_tag="ACHLIF_005404"
     CDS             351..>1286
                     /gene="wzy"
                     /locus_tag="ACHLIF_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O26 family O-antigen polymerase"
                     /protein_id="HFO9609183.1"
                     /translation="MYYIIFVMVLGLWIIAFNYARANKLSNLVIVLIITTLFIINRQN
                     QDYEAYVDIFNVNELYAEIGYRWLIYGVKYLGGTHEVIIGLLGLFLGTTFLRLIQYSK
                     YTAFGLLLYMLCPMPIDIVQIRNTFLFLFVINSLIELEKEHKFNSLCFVFMAPLFHSL
                     GIVYVLAWVIIQFRTWRGYNKLMVFGLVLSFIVVPLLIKMLILLFNTRTLHAYIADGI
                     KVHSLIIWAGPYLFDLFLLCYFRKKIVITDLHTKQWIDIIFSLMLFLSVFSPLLLYID
                     EINRIFRNALLLKYLVMMSIAQYLSRPTRYILYSYL"
BASE COUNT          372 a          181 c          228 g          505 t
ORIGIN      
        1 caaagtatcg ctgaaattag aagcgcgttc atccctttat caataatgtc ttttgcaata
       61 tttatgggga tatacaattt tatgtttggt tcggttggat tgtccataag agggtataaa
      121 aaagaatttt cttatatagt ggccattacg ggtgtttcaa ctattatttt atcattatgc
      181 ctgagttatt tctttgctga aataggcgct gcaattgctt atgtatttgc tgagtttatc
      241 ttacttattc tcatacttag aatttataaa gtgaaacgat tataattccc ggtaagaact
      301 gatagtgcaa aatcatcatg gctatagact gaataaattg cggggaaaga atgtattaca
      361 tcatatttgt gatggtttta ggtttatgga ttattgcatt caactacgct agagcaaaca
      421 agctaagtaa tttggtaatt gtgcttatta ttactacttt atttattatt aataggcaga
      481 atcaagacta tgaagcgtat gttgatatat ttaatgtcaa tgaactttat gccgagattg
      541 gttatcgctg gttaatttat ggtgttaagt atttaggcgg tacccatgaa gtcataattg
      601 gcttgctggg tttattcctt gggaccacat tcctacgatt aatacaatac agtaagtata
      661 cagcatttgg tttgctgctt tacatgttgt gtccaatgcc tattgacatt gttcaaatca
      721 ggaatacatt tcttttttta tttgtcataa attctcttat cgagttagaa aaagagcata
      781 aatttaactc tctttgtttt gtctttatgg cacctctttt ccatagttta ggcattgttt
      841 atgtattggc gtgggtaata atccaatttc gtacctggag gggttataat aagttaatgg
      901 tttttggact ggtgctgtct tttattgtag tgccactctt gattaaaatg ttaattttat
      961 tatttaacac taggacgtta catgcatata tagctgatgg cattaaggtt cattctctta
     1021 ttatatgggc aggtccttat ctttttgatc tttttctgtt atgttacttc agaaaaaaaa
     1081 tagtaataac tgatttacat actaaacaat ggattgatat aatattctcg ctaatgttat
     1141 ttctttccgt attttcacca ctacttttat atatcgacga aataaatagg atttttcgaa
     1201 atgcgctatt actgaagtat ctggtaatga tgtctatcgc acagtattta agtagaccga
     1261 ccagatatat actttattca tattta
//
LOCUS       DAZGOB010000363         1286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGOB010000363 DAZGOB010000000
VERSION     DAZGOB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.366"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..763)
                     /locus_tag="ACHLIF_005405"
     CDS             complement(<1..763)
                     /locus_tag="ACHLIF_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9609184.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAITAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLAPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATYAQQPALFTAML"
     gene            complement(1170..>1286)
                     /locus_tag="ACHLIF_005406"
     CDS             complement(1170..>1286)
                     /locus_tag="ACHLIF_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO9609185.1"
                     /translation="VMVDFTSRIMSVLADGVLVCGIVVLLWPVIKRNSLHNA"
BASE COUNT          307 a          381 c          301 g          297 t
ORIGIN      
        1 tcagcatggc tgtaaacagc gcaggttgct gtgcgtaggt ggcggcgctc atgtcaaact
       61 ctccctgcat ccagcacacc gccagcaaca catttttcgg gttcttctgt aatgcagctt
      121 tagtgcgcgc aatcaggtcc tgatataacg gtttacccac accccagcgt gccgaatcct
      181 ggctggcccc cgtgtccgca ctgaatgtcc cctccgcgcc ctgggtgaat gccgaaccac
      241 cacgacagca tggtgccagc aggatccccg cgttattcgg gatatacgga agcagttttt
      301 tggcaatatg taagccctgg ccgacacagc cgtactgccc tttgctcagg tctgccttcg
      361 gatgattcag cgtactcata tcctgcacat catgcaggca gtggtcggcc ggaataatat
      421 cgttatatct gcaggcagcc ccacccggcg tcactgtact gcggcgcgcc agctgtttaa
      481 tgcgcggatc cggagcatcg tatgaatccg gcagcggaag cccttcaccg taagccatgg
      541 cattggactg cccggccagt acgatgacgt agtaccactc cggctcagtt gcaccactga
      601 ccaccacatc accttctgct gtaatcgcct gcatcagggt ataaggggtt atggccaccg
      661 gactcccaaa cggctgccag ccctctttca gtttgtgtgt cagcttttcc gcaaggtctg
      721 acggcgacgc cgccctgaca acatcataat gtttaaatgt cattattcct cccggccggg
      781 atagtgtatt aaatcagata tggagtgggc tgtagtccgg aagcctgaat gacacacggg
      841 gactacagcc caagaaatga aaaaaggcca cgcagttgcg cagcctgata aaccctggtt
      901 aaaatccaca cgataacaac acaacaatat cagtatctca tgctattgcc cgaacccatt
      961 cgggcatttt ttacccataa aaaagcccct ccggagaggg gcatgtttgc atgcacattc
     1021 tttttcttgc atggtgccgg gtgcctcccg gtgaattcag tatcagcacc tgaatccgcg
     1081 attatcacat atacctggtt gctgattgcc cctccgcaca gggggattca ccatgcagta
     1141 gtatttttaa taaacagtaa acaaaaaaat caagcattat gcaggctgtt tctttttatc
     1201 accggccaca gcaataccac aatgccgcag accagcaccc catccgccag caccgacatg
     1261 attctgctgg tgaaatccac catcac
//
LOCUS       DAZGOB010000364         1273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGOB010000364 DAZGOB010000000
VERSION     DAZGOB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.367"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1037)
                     /locus_tag="ACHLIF_005407"
                     /pseudo
     CDS             complement(1..1037)
                     /locus_tag="ACHLIF_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(1046..>1273)
                     /locus_tag="ACHLIF_005408"
     CDS             complement(1046..>1273)
                     /locus_tag="ACHLIF_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO9609186.1"
                     /translation="TIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTVKSALAF
                     LILIIYFAPILAERVMPLSFFPEQLQLYIEK"
BASE COUNT          418 a          243 c          210 g          402 t
ORIGIN      
        1 ttgaacaggt tgcccagcat tctccacatc ttcaagccac agcagaagac gtaatatttc
       61 atctatattt tcgaagctaa cataatcgta gcgacgatgg gtagcataat tttttcgtgc
      121 taatttttta tctgtgataa ttggtattcc gatttccttt gcatatttcc ttacgcctaa
      181 ggccacctca ttagttactc ttacagaaat caatggaata ggtgaaagat gtggcttaaa
      241 gtatatccct attgcaatgt gagtagggtt ggaaatcatc aaacgactat tactgacatc
      301 agacttcaat tgctcagaaa gaatttcctg atgcgctctc tgcgtttaga cttaatttct
      361 ggatttcctt cttgctcctt gtattctctt ttaacttctt gtttatccat tttcatatct
      421 ttcataaata aaaaatattc agcgataaaa tcaaaaatta agacaattat catactgccg
      481 agacaataca gtatgagaag gaatagcatc tctccccaat ctgataataa agatatgata
      541 tctccatcaa gagttttaaa aagcagtgat ttatgattac tccaaaatac tttgatcgcc
      601 aatgcaaaaa aaacaatata aagaattgct ttaacaaatt cttttacggt ttttaagcca
      661 aatatacgct ttaagccatt tactggattc aatgaatcaa acttaaattt aacagcttca
      721 gttgctaatt gcattttact ttgaaaccag gagacaagta ttgttgtgaa catgcaaacc
      781 agcaaaaaag gaatgttcgt tttcaaccaa ccaaccccca tagctttaaa atagtcccaa
      841 atatctggtt tagcgttatg atcaaggatg tattcaagaa tcgacataat gtgatgcaca
      901 tcaaaaacat atccgagata taaagtaccc acaagcatta ttacagagat ggttaagtct
      961 ctactttata gaaattgccc ttttttagaa gcatcctgga gtttcttttg tgttggcttt
     1021 tcagttttat tggccatgaa acactctatt tctcaatgta tagttgcaat tgttctggga
     1081 aaaaggataa cggcattact cgttcagcca gtattggggc aaaataaata attaatatta
     1141 aaaatgccag cgcacttttg acagtcagtg aaatggcaaa agcatttagt tgtgaagcat
     1201 aacgtgccag caatcccagg acagcctcac cgcccagcat aacggcaatc acggggcttg
     1261 cgtaaacgat agt
//
LOCUS       DAZGOB010000365         1272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGOB010000365 DAZGOB010000000
VERSION     DAZGOB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.368"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            10..1044
                     /gene="fnlA"
                     /locus_tag="ACHLIF_005409"
     CDS             10..1044
                     /gene="fnlA"
                     /locus_tag="ACHLIF_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     4,6-dehydratase/5-epimerase"
                     /protein_id="HFO9609187.1"
                     /translation="MFKNKTLVITGGTGSFGNAVLKRFLDTDITEIRIFSRDEKKQDD
                     MRKKYNNSKLKFYIGDVRDYNSVLNATRGADFLYHAAALKQVPSCEFHPMEAVKTNVL
                     GTENVLEAAIANGIKRVVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVIC
                     GTRYGNVMASRGSVIPLFVDLIKAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIQTLAIALKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDM
                     GDYYRVPPDLRDLNYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKELLLKLPFIRAL
                     RSGEDYELDS"
     gene            1046..>1272
                     /locus_tag="ACHLIF_005410"
     CDS             1046..>1272
                     /locus_tag="ACHLIF_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO9609188.1"
                     /translation="MKILVTGAAGFIGRNLVFRLKEAGYNELIAIDRNSSLTDLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNCDLTQQ"
BASE COUNT          390 a          211 c          291 g          380 t
ORIGIN      
        1 ggtgaaaata tgtttaagaa taaaacactc gttatcactg gtggcactgg ttcttttggt
       61 aatgccgtac ttaagcgttt tctagataca gatattactg aaatacgaat atttagcagg
      121 gatgaaaaaa aacaagatga tatgcggaaa aaatataata actcaaaatt aaaattttat
      181 ataggtgatg tgcgagacta taattccgtt ctaaatgcaa cgcgtggtgc cgattttctg
      241 tatcatgcag cagcccttaa acaagttcct tcatgtgaat ttcaccctat ggaggcggtt
      301 aagacaaatg ttctgggtac ggaaaatgtt ctggaggctg ctattgcgaa tgggattaaa
      361 cgcgtggtgt gcttgagtac cgataaagcc gtttatccta tcaatgcaat gggcatatct
      421 aaggcaatga tggaaaaagt tattgttgca aaatcacgta atcttgacag ttcaaaaaca
      481 gttatctgtg gaactcgtta tggaaatgta atggcttcac gtggatcggt catcccatta
      541 tttgttgatc taatcaaagc tggtaaacca ttgaccataa ccgatcccaa tatgactcgt
      601 ttcatgatga cgcttgagga tgctgtcgat ctggtccttt atgctttcga acatggaaat
      661 aatggtgaca ttttcgttca gaaagcacct gcggcaacaa ttcaaacatt agccattgca
      721 cttaaggaat tgctaaatgc ccatgagcat ccaatcaata ttattggaac tcgacacggg
      781 gaaaaacttt acgaagcgtt attgagccga gaggaaatga ttgcagcgga agatatgggt
      841 gattattatc gtgttccacc agatctccgc gatttgaact atggaaaata tgtggaacat
      901 ggtgaccgtc gtatctcgga agtggaagat tataattctc ataatactga gagattagat
      961 gttgagggaa tgaaagaatt actgctaaaa cttcctttta tccgggcact tcgttctggt
     1021 gaagattatg agttggattc ataatatgaa aattttagtt actggcgctg cagggtttat
     1081 cggtcgaaat ttggttttcc gccttaagga agctggatat aacgaactta ttgcgataga
     1141 tcgtaactct tctttgacgg atttagagca gggacttaag caggcagatt tcatttttca
     1201 tcttgctgga gtaaatcgtc ctgtgaagga gagtgagttt gaagagggga attgcgatct
     1261 aactcaacag at
//
LOCUS       DAZGOB010000366         1266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGOB010000366 DAZGOB010000000
VERSION     DAZGOB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.369"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1266
                     /locus_tag="ACHLIF_005411"
     CDS             <1..>1266
                     /locus_tag="ACHLIF_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO9609189.1"
                     /translation="STPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKET
                     PFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGE
                     EIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGER
                     PDAEVMAERKEYYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMG
                     RHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIP
                     IKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFP
                     NNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISR
                     WQLDLSALLASLQEEDGAATNKKTLAEYAR"
BASE COUNT          317 a          321 c          371 g          257 t
ORIGIN      
        1 ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg aatccccgca
       61 ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc ttaaatttgg
      121 cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca gcgtgtttta
      181 tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta ctgatgcccg
      241 ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt tttcgtcatc
      301 cggtgaagag attgaaccgc ctgacagtgt gacctttcac atctggacgg cgtacagccc
      361 gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaag acgaaagggg atacgggaaa
      421 acgtaaaacc ttcgtgaaca ccacgctcgg tgagacgtgg gaagcgaaaa tcggcgaacg
      481 tccggatgct gaagtgatgg cagagcggaa agagtattat tcagcgcccg ttcctgatcg
      541 tgtggcttac ctgaccgccg gtatcgactc ccagctggac cgctacgaaa tgcgcgtatg
      601 gggatggggg ccgggtgagg aaagctggct gattgaccgg cagattatta tgggccgcca
      661 cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct atacccgccg
      721 gaatggtgca gaaatgtcgg tatcccgtat ctgctgggat actggcggga ttgacccgac
      781 cattgtgtat gaacgctcga aaaaacatgg gctgttccgg gtgatcccca ttaaaggggc
      841 atccgtctac ggaaagccgg tggccagcat gccacgtaag cgaaacaaaa acggggttta
      901 ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct tcacactgac
      961 gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc cggatatttt
     1021 tgatcttacc gaagcgcagc aactgactgc tgaagagcag gtcgaaaaat gggtggatgg
     1081 caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg actgcttcgt
     1141 ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc tcagtgcact
     1201 gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac tggcagaata
     1261 cgcccg
//
LOCUS       DAZGOB010000367         1265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGOB010000367 DAZGOB010000000
VERSION     DAZGOB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.370"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..643)
                     /locus_tag="ACHLIF_005412"
     CDS             complement(<1..643)
                     /locus_tag="ACHLIF_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9609190.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKF"
     gene            complement(643..972)
                     /locus_tag="ACHLIF_005413"
     CDS             complement(643..972)
                     /locus_tag="ACHLIF_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609191.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(969..>1265)
                     /locus_tag="ACHLIF_005414"
     CDS             complement(969..>1265)
                     /locus_tag="ACHLIF_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9609192.1"
                     /translation="FVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQASG
                     TFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          266 a          420 c          303 g          276 t
ORIGIN      
        1 ggaacttaca accgctcagg catttgctgc atttatcctt cgtgatatcg gacgttggct
       61 ggtcatattc atccgcgaca gccggaccgc tataaccgca ctcgtcaccg cgataggtcc
      121 aggtgcaggt gttggccagc atgatacgtc ccggaaaaac agcgccatcc gtttccgtcg
      181 gcgtggacag tacaaaggag gcactcaccg cgctcagttc gctgcactgc tcaatgcgcc
      241 agcggctgat cacctcctgc tccggatcgg cgtaactgtt tccgttgacg aagttcaccg
      301 catccagaaa acgggcgtaa accttacgcc tgaccaccgt tccgccgacc agactctgca
      361 tatcttccac cataccggtg accatgccgt gcaggttaga aaccgtcagt gtcggacggg
      421 cagcactgcc cttgccgttc agttcaaatc ccgtcccctg aatgggatac gcctgatact
      481 gtcgcccctg ccaggtgacc ggctcacctt tttcgttctg ctcattacag aaaaaataac
      541 gttctccacc gacctctgtc aggtcgattt cccagagcac cacgctggcc gactgctccg
      601 cacgggtgca ttcattcagt gtttcctgcc ggatatcctg catcagttca ccacctgttc
      661 aaactctgcg ctgaactcaa cacgcagcat actgacccgc gacgaccatt ttgcgcaggt
      721 cacctttatc tgccgccact cataaggcgg cgtccacaga aaggatttcc agcccccgtg
      781 ctcttccaga aacgactcca gtaccgtggc ctcctcacgg gggacagaaa gcgtcacgct
      841 gtacgttttc aggttggcat tcagcccggc aggcgctcgc tgggaatagc catcaccaaa
      901 gcgcaccttt cttacagaag gggccgaagc cacatccata ccgggtttca ctttccagcg
      961 gaaggttttc atcgtccacc tccggagaac aggccaccat cacgcatctg tgtctgaatt
     1021 tcatcacggg cacccttgcg ggccatgtca tacaccgcct tcagagcagc cggacctatc
     1081 tgcccgttcg tgccgtcgtt gttaatcacc acatggttat tctgctcaaa cgtcccggac
     1141 gcctgcgacc ggctgtcagc cagactgccc ggtgtaccga cataaccacc ggtggcatag
     1201 ccgcgcatca gccggtagag attccccacg ccaatccggc tggttgcctc cttcgtgaag
     1261 acaaa
//
LOCUS       DAZGOB010000368         1265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGOB010000368 DAZGOB010000000
VERSION     DAZGOB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.371"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..643)
                     /locus_tag="ACHLIF_005415"
     CDS             complement(<1..643)
                     /locus_tag="ACHLIF_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9609193.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKF"
     gene            complement(643..972)
                     /locus_tag="ACHLIF_005416"
     CDS             complement(643..972)
                     /locus_tag="ACHLIF_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609194.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRVPAGLNADLKTY
                     SVTLSVSREEATALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSQVSMLRVEFSA
                     EFKQVVN"
     gene            complement(969..>1265)
                     /locus_tag="ACHLIF_005417"
     CDS             complement(969..>1265)
                     /locus_tag="ACHLIF_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9609195.1"
                     /translation="FVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASG
                     TFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          265 a          421 c          307 g          272 t
ORIGIN      
        1 ggaacttaca accgctcagg catttgctgc atttatcctt cgtgatatcg gacgttggct
       61 ggtcatattc atccgcgaca gccggaccgc tataaccgca ctcgtcaccg cgataggtcc
      121 aggtgcaggt gttggccagc atgatacgtc ccggaaaaac agcgccatcc gtttccgtcg
      181 gcgtggacag tacaaaggag gcactcaccg cgctcagttc gctgcactgc tcgatgcgcc
      241 agcggctgat cacctcctgc tccggatcgg cgtcactgtt tccgttgacg aagttcaccg
      301 catccagaaa acgggcgtaa accttacgcc ggaccaccgt tccgccgacc agactctgca
      361 gatcttccgc catcccggtg accataccgt acaggttaga aaccgtcagc gtggggcgcg
      421 tactggtgcc tttgccattc agttcaaaac cgctcccctg aatgggatac ggctgatact
      481 gtcgcccctg ccaggtgacc ggctcacctt tttcgttctg ctcattacag aaaaaataac
      541 gttcaccacc gacctctgtc agatcgattt cccagagcac cacgctggcc gactgctccg
      601 cacgggtgca ttcattcagt gtttcctgcc ggatatcctg catcagttca ccacctgttt
      661 aaactctgcg ctgaactcaa cacgcaacat actgacctgc gacgaccatt ttgcgcaggt
      721 cacctttatc tgcctgtaac cataaggcgg cgtccacaga aaggccttcc agcccccgtg
      781 ctcagccaga aacgactcca gcgccgtggc ctcctcacgg gagacagaca gcgtcacgct
      841 gtacgttttc aggtcagcgt tcagcccggc aggcacacgc tgggaatagc catcaccaaa
      901 gcgcacctcc ctgacggaag gggccgatgt cacatccata ccgggtttca ctttccagcg
      961 gaaggttttc atcgtccacc tccggagaac aggccaccat cacgcatctg tgtctgaatt
     1021 tcatcacggg cacccttgcg ggccatgtca tacaccgcct tcagagcagc cggacctatc
     1081 tgcccgttcg tgccgtcgtt gttaatcacc acatggttat tctgctcaaa cgtcccggac
     1141 gcctgcgacc ggctgtctgc catgctgccc ggtgtaccga cataaccgcc ggtggcatag
     1201 ccgcgcatca gccggtaaag attccccacg ccaatccggc tggttgcctc cttcgtgaag
     1261 acaaa
//
LOCUS       DAZGOB010000369         1257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGOB010000369 DAZGOB010000000
VERSION     DAZGOB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.372"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..630
                     /locus_tag="ACHLIF_005418"
                     /pseudo
     CDS             1..630
                     /locus_tag="ACHLIF_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
     gene            complement(641..712)
                     /locus_tag="ACHLIF_005419"
                     /pseudo
     CDS             complement(641..712)
                     /locus_tag="ACHLIF_005419"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            775..921
                     /locus_tag="ACHLIF_005420"
                     /pseudo
     CDS             775..921
                     /locus_tag="ACHLIF_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            948..1052
                     /locus_tag="ACHLIF_005421"
                     /pseudo
     CDS             948..1052
                     /locus_tag="ACHLIF_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          361 a          283 c          296 g          317 t
ORIGIN      
        1 cactgccgga catcagcgct atctctgctc tccttgccgt aaaacatggc aactacagtt
       61 cacttacacc gcctctcagc ccggtaagca ccagaaaatc attgatatgg ccatgaatgg
      121 cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg ttttacgtca
      181 cttaaaaaaa tcaggccgca gtcggtaacc tcgcgcatac aaccgggcag tgatgtgatt
      241 gtctgcgctg aaatggacga acagtggggc tacgtcggtg ctaaatcacg tcagcgctgg
      301 ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtctt cggtgaacgc
      361 actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt cgtggtatag
      421 atgacggatg gctggccgct gtatgaatca cgcctgaagg gaaagctgca cgttatcagc
      481 aagcgttaca ctcagcgcat tgagcgacat aatctgaatc tgagacaaca tctggcaagg
      541 ctgggacgga agttactgtc gttctcaaaa tcggtggagt tgcatgacaa ggtcatcggg
      601 cattatctga acataaaaca ctatcagtaa gttggagtca ttacctcctc ccgaactcaa
      661 agctatcata cagataatta ctggttaacc ctatataggg cattttaaag tgttgtgatc
      721 ttacccagca atagtggaca cgcggctaag tgagtaaact ctcagtcaga ggtgattcac
      781 aagacaaaac agtttcaacc agtaaaaaaa cgtaaacacc attcgcatga atttcgtagt
      841 taagatctga agcttgcaga atgcatcggt gttactgccg gagttcgtga acttagtctg
      901 catgaatcac cacagcttta catctgtcgc agtaaacagc aaaatcagca aacttactta
      961 attatatcga gtgcgattac aatcgctggc gatggtacag ttggtgtggc ggtctcagtc
     1021 cggaacaatt tgaaaatcag aatctcactt aaaacggcat ccattttaca aaagatggcc
     1081 gtctgtgtat ttgtaatgaa taccttctac atgtttagag tgcataatcc acgacaaggc
     1141 gatatcctaa aaattagcta gcaggcaact ttgatggcat agataatgtt tttagtaaaa
     1201 aagagttgac tgaatataat acagcctctt cctacataag aaggaatgtg ctcacaa
//
LOCUS       DAZGOB010000370         1252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGOB010000370 DAZGOB010000000
VERSION     DAZGOB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.373"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(288..1163)
                     /locus_tag="ACHLIF_005422"
     CDS             complement(288..1163)
                     /locus_tag="ACHLIF_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO9609196.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTPGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
BASE COUNT          351 a          251 c          239 g          411 t
ORIGIN      
        1 gacaccataa gtcagcattt cataattatg gatgcagacc aacagaaaca gcattgtaaa
       61 taaaatgtaa taattacata ctattagtga ttctcatgca tcgtaagcgg ctcgccagaa
      121 ccgtattgat atttactgag cgctcagatc aactttccat ggcaacagat cgcgtacccg
      181 gtttgccggc cagtcctgga tatgctcccg ccgacaatat taatggccag gaaatgttta
      241 aaacaatgat tctgtttcct aaccatcaag tgtatatgct ggctcaatta ctcaatatct
      301 atgggttgag ttctacccaa agtggctctg tagtcgcttc ttcttacagt atgttcaggt
      361 gttgcaggca cagttgtgga gcgtagatgc gttgttggtt taccatgctc tggtacataa
      421 aaaacgccag tgccatggta acctgatata gaaacgttaa ttccgcgacg ttcaaactcg
      481 gcatagacgt gttctgctaa agacctcttt tgaccaaata atgccctggc caaccagccg
      541 ttattcatat ttgcggattt ttctatttca tcaggagaga agtctttgtt ttttattatg
      601 ttggctgagt tacaggatgt taatctgata tcatctattt catgaagatt atgttgaaca
      661 atgcggtcga caatatctga cggtgataaa aattcatctc cgcacttaag aagaggtaga
      721 ccagcagaac catgcccaga cagataaatt ttgttgtatt ttcctggtgt taaatcatct
      781 ggcaggatgg ttttcatctc tgtcgctgta atacttatag ctgcagcagc aacaacatcg
      841 ctattacttg attgtagatg gcttttattt gctccaggat atgtaaactc cattttcctt
      901 ttatcaaaat cttgttttgt agcatcattc aggaataata aatcatacgg ttttttcccc
      961 gttgtgactc ttgaaacatt ttggccaaga atattaagag tatagtcatt aacggatttc
     1021 attcctaaaa aaacaagtaa tccttctttc tcttgcgctt tttcgattgt tctccctgct
     1081 aaagttattg ggatggattg agattgtttt gtcggaactg atacgctggc gctaataggt
     1141 agtagggttc ttatgctaaa catacaacct ctttcttaat tttcgaacct gtttaggatt
     1201 ctgtgtaaat tcaaaataaa cctgtctgaa cattcctaac aaacatccac cg
//
LOCUS       DAZGOB010000371         1245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGOB010000371 DAZGOB010000000
VERSION     DAZGOB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.374"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            72..1198
                     /locus_tag="ACHLIF_005423"
                     /pseudo
     CDS             72..1198
                     /locus_tag="ACHLIF_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          444 a          175 c          289 g          337 t
ORIGIN      
        1 ttggctttaa cgcctacagg tgatcaaaat ttaacctttg gaataactaa aaagataaaa
       61 aaggacgcca ggtgagtatt caaagtttgc ttgattatat ttcagtgatc cctgatatac
      121 gacaacaagg aaaggttaaa cataaattat ctgctatttt gtttctcacc gtatgtgcag
      181 taattgcagg tgccgatgag tggcaggaaa ttgaagattt tggacatgaa agacttgaat
      241 ggctaaagaa atatggtgat tttgataatg gcattccggt cgatgacacc attgcacgcg
      301 ttgtgagtaa cattgacagt ttggcctttg aaaagatgtt tattgaatgg atgcaggagt
      361 gccatgaaat cactgatggt gaaattatag caatagatgg aaagaccata agaggctcct
      421 ttgataaggg aaaaagaaaa ggagcaatcc atatggtgag tgcattctcg aacgaaaatg
      481 gtgttgtact ggggcaggtg aaaacggaag ccaaaagtaa tgagattaca gccattccag
      541 agttgcttaa cctactgtat ttaaagaaaa atttgataac cattgatgct atgggctgtc
      601 agaaagatat cgcttcgaag atcaaagata aaaaagcaga ttatcttctg gcagtaaaag
      661 gcaatcaggg gaaattacat catgcattcg aggaaaaatt tcctgtaaat gtgttttcta
      721 attataaagg cgattcgttt agtacgcagg agataagtca tggaagaaaa gaaacacgtt
      781 tgcatattgt cagtaacgta acgcctgaac ttttgtgatt ttgaattcga atggaaggga
      841 ttaaaaaagc tttgtgtagc attgtcattc aggcagaaga aagaagataa atcagcagaa
      901 ggtgtaagca tccgatatta tatttcatca aaggatatgg atgctaaaga atttgcacat
      961 gctatcagag cgcactggct gatcgagcac agtcttcatt gggtgttaga tgtaaaaatg
     1021 aatgaagatg ccagccggat aagaagagga aacgcagcct aaataatatc tggaataaag
     1081 aagatggcac tgaatttatt aagggattgc aaagacatta agggtgaaga ggaaaagaaa
     1141 gaaggctgcg ttaaacacag ggaaagatca tcagaagttc actttttgta ctaaataatt
     1201 cgcattttat gtttaaaaat tgagatattc ctcattacct gaagc
//
LOCUS       DAZGOB010000372         1208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGOB010000372 DAZGOB010000000
VERSION     DAZGOB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.375"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..816
                     /locus_tag="ACHLIF_005424"
     CDS             <1..816
                     /locus_tag="ACHLIF_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609197.1"
                     /translation="EIHVPMEMVVSDGEERFTELDFYYGSKALEGTSEIVTVLTNTIL
                     NREVVTKIPSIEGITASFRTSYEGSFRQRFMLKITGEEQRRNYGAIGEGAFLDVMSYY
                     LTRPLLTPYNFTYKRAARIVKELESDYSSLMKRLYGPLLKLHKPIEEQNYTVELKRRR
                     TNLARYDHTTLENLIVERADDERIIIEASITRFNRLTGTGRLILEEDSESVSFEPSYL
                     WRDFPADQKRWLSRNLDVNTDNGDFTRVRLEVTSIRNFLGVIKKYSLHRVITD"
     gene            818..>1208
                     /locus_tag="ACHLIF_005425"
     CDS             818..>1208
                     /locus_tag="ACHLIF_005425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609198.1"
                     /translation="MIKYVAFLVISVTWLIYFFNFDPLNGFPIDKSEWGTFGDFVGGV
                     TNPILTFFTMIMLIKSINLQKEANISIIKQNLHMHEVSERQKEIDDLRSFESSFYSLA
                     EVSRREFDALRITGRNKKNYSASDAVTY"
BASE COUNT          399 a          203 c          243 g          363 t
ORIGIN      
        1 gaaattcatg ttcccatgga aatggtagta agtgatggag aagaaagatt cacagagctt
       61 gatttttact atgggtcaaa agcacttgaa ggcacgtcag agatagttac agtcctcact
      121 aatacgattc taaatcgcga agtagttact aaaatcccat caatcgaagg cataaccgca
      181 tcttttcgta ccagttatga agggtcgttt agacaacgtt tcatgctcaa gatcacaggt
      241 gaggaacaac gtagaaacta tggcgcaatc ggagaggggg catttctaga tgttatgtct
      301 tattatctta caagaccatt attaacacct tataatttca catataaaag ggctgctaga
      361 attgttaaag aacttgagtc agattatagt tcattaatga agaggcttta tggcccacta
      421 ttgaagcttc ataagcccat agaagaacaa aattatactg ttgagctgaa acggagaagg
      481 acaaatcttg ctcggtatga tcacacgacc cttgaaaatc taattgttga acgtgccgat
      541 gatgaaagga tcattattga agcttctatt acaagattta atcggctaac ggggacggga
      601 aggctcattc ttgaggaaga ttctgagtct gtaagttttg aaccttcata tctttggaga
      661 gattttccag cagatcaaaa acgctggcta tctagaaact tagacgtcaa cacagataat
      721 ggtgatttta ctcgtgtacg gctagaggtg acatcaatca gaaactttct tggtgtcatt
      781 aaaaaatata gtcttcaccg agttataacg gattgatatg ataaagtatg ttgccttttt
      841 agttatatca gttacatggt tgatttattt cttcaatttc gatccgttaa atggttttcc
      901 aattgataag tctgaatggg gaacatttgg tgatttcgtt ggcggcgtaa ccaaccccat
      961 tttgacattt tttacaatga ttatgttaat taaatcaata aacttacaaa aagaggctaa
     1021 catcagcatt attaaacaaa atttgcacat gcatgaggtt tcagaaagac aaaaagaaat
     1081 tgatgacttg cgctcttttg aatcatcgtt ttatagcctc gctgaagtat ctagaagaga
     1141 gtttgatgcc ctcagaataa ctggtagaaa taaaaaaaac tactcagcct cagatgcggt
     1201 aacctatt
//
LOCUS       DAZGOB010000373         1192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGOB010000373 DAZGOB010000000
VERSION     DAZGOB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.376"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..832
                     /gene="yeeO"
                     /locus_tag="ACHLIF_005426"
     CDS             <1..832
                     /gene="yeeO"
                     /locus_tag="ACHLIF_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO9609199.1"
                     /translation="GLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKP
                     LNFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLP
                     GSALGSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYT
                     QDPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVG
                     YVLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE
                     "
     gene            933..1008
                     /locus_tag="ACHLIF_005427"
     tRNA            933..1008
                     /locus_tag="ACHLIF_005427"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Asn,seq:gtt)
BASE COUNT          257 a          240 c          326 g          369 t
ORIGIN      
        1 gggactggga tttgtcgggg ctgggttggg tttaaccatt tctcgttata ttggcgcagt
       61 tgcaattttg tgggtgctgg cgattggttt taatcctgcg ctaaggattt cgttaaagag
      121 ctattttaaa ccgctgaatt ttagcattat ctgggaagtc atggggattg gtattccagc
      181 gagtgtcgaa tcagtgttat ttaccagtgg tcggttatta acccaaatgt tcgttgccgg
      241 gatggggacc agtgttattg ccggaaattt tatcgcgttt tcaattgcgg ctcttatcaa
      301 cttacccgga agtgcgctcg gctctgcttc tacgatcatt acaggccgaa ggttaggggt
      361 agggcagata gcgcaagcag agattcagtt gcggcatgtc ttctggctgt ccactcttgg
      421 attaacggcc atcgcctggc taacggctcc ctttgccggg gttatggcat cgttttacac
      481 ccaggatcca caggttaaac atgtcgttgt gattctgatt tggctaaatg ctttatttat
      541 gcctatttgg tccgcctcat gggtgctacc cgctggattt aagggggctc gtgacgcccg
      601 ttacgctatg tgggtttcta tgttgagcat gtggggttgc cgggttgtcg tcggttatgt
      661 gctgggcatc atgcttggct ggggtgtggt tggtgtctgg atgggaatgt ttgccgactg
      721 ggctgtgcgg gccgtgctgt tttactggcg aatggttact ggacgttggc tatggaaata
      781 ccctcgaccc gaaccgcaaa agtgtgaaaa aaagccagtt gtgtcggaat aaacaacaaa
      841 atgcagatta tttcagcaaa cgatttcaaa tttaaaaaac aggctttgac attgtgggtg
      901 ggcatcgcta atattcgcct cgttctcacg attcctctgt agttcagtcg gtagaacggc
      961 ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa ttttagtttt
     1021 gagacatctc tatgagtctc aaggcagtta aaaaataaaa attattttta cccggtcagt
     1081 ttgtaaaaaa gtggccgggt tttttgattt ctgtgtttcg tgcgcttaat cgagtctcac
     1141 gcttcagtga gcaaattgct cctccccaac agacatcacg attcacaagg tc
//
LOCUS       DAZGOB010000374         1189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGOB010000374 DAZGOB010000000
VERSION     DAZGOB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.377"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..756)
                     /locus_tag="ACHLIF_005428"
     CDS             complement(<1..756)
                     /locus_tag="ACHLIF_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609200.1"
                     /translation="MLKSFCLNFDGMYLSIVSFLSEKFNLKRVGKMELESKEDYEETT
                     HHNVASSVVTATHDNLDGREFFELCSNPALVFSFETHKKFNELPVDSKESPLDLFIAN
                     TKQLHLLRSGDISNTLSHLLLLGYVSAAESYLRALIRNLINHDEYIQSIVSDKAVSYA
                     AAQHHKKELLPEALLEDFSLASPYNVFETLKDIIGMKGQRPIEMMKCSSEFMKVCELR
                     HCCVHRFGKLGSKNAIRLGLAEHMKHLEKPIILN"
     gene            complement(734..>1189)
                     /locus_tag="ACHLIF_005429"
     CDS             complement(734..>1189)
                     /locus_tag="ACHLIF_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9609201.1"
                     /translation="ERVFNEGSRGFSPSQIIQKILRKNAKHPVRRNISSLFNAEQLAR
                     LKLTLPPCNNCKTPRLTDNQKFCHHCGNQLIDESAYNQCMSIPLSKVPHLTSWQMQKL
                     SGLEKVKTIGDVLSLQDPGSELRKIHRIGPKIANKIMDKVLLHVEEFLS"
BASE COUNT          358 a          228 c          195 g          408 t
ORIGIN      
        1 attgagtatt atcggttttt ctaagtgctt catatgttca gccaatccta gtcggattgc
       61 atttttcgaa cccaacttac caaatcgatg aacacaacaa tgccttaact cgcaaacttt
      121 catgaattcg cttgagcatt tcatcatttc gattggtcgt tgtcctttca ttccaataat
      181 gtcctttaat gtttcaaaaa cattataagg gcttgccaat gaaaagtcct caagtaaagc
      241 ttctggtaat aactcctttt tatggtgttg tgccgctgca taactaacgg ccttatcaga
      301 aactatagac tgtatatatt catcatgatt aattagattt ctgataagag ctcgcaaata
      361 actttcggct gcagacacat aacctaataa caacagatgg cttaatgtgt ttgaaatatc
      421 accagatctt aacaagtgaa gttgctttgt attagctata aaaagatcta acggggattc
      481 cttgctatca acaggtaact cattaaattt tttgtgtgtc tcaaacgaaa aaactagagc
      541 tggattacta catagttcaa aaaactctct tccatctaag ttatcatggg ttgcagtgac
      601 aactgaggac gccacattat gatgagtggt ctcttcataa tcctcttttg attctagttc
      661 cattttcccg actcttttta aattaaactt ttcagataag aaagacacga ttgacaagta
      721 catgccatca aaattaagac aaaaactctt caacatgtaa taacactttg tccataattt
      781 tattggcaat tttgggtcct attctgtgaa tttttcttaa ctcactccca gggtcttgta
      841 aagacaatac atctcctata gtttttactt tttccagccc agatagtttt tgcatttgcc
      901 aagatgttag atgtggtact tttgacagtg gaatagacat gcactgatta taggccgact
      961 catcaatcaa ttgattccca caatgatggc aaaatttttg attgtcagtc aatcgcggag
     1021 tcttgcagtt attgcatgga ggtaaagtta gtttaagtct cgcaagttgc tcagcattga
     1081 ataatgatga aatgtttctt ctgacaggat gcttagcatt ctttcttagt attttttgaa
     1141 taatctgaga aggagaaaaa cctctacttc cttcgttaaa cactctttc
//
LOCUS       DAZGOB010000375         1189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGOB010000375 DAZGOB010000000
VERSION     DAZGOB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.378"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..567)
                     /locus_tag="ACHLIF_005430"
     CDS             complement(<1..567)
                     /locus_tag="ACHLIF_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609202.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLL"
     gene            complement(654..860)
                     /locus_tag="ACHLIF_005431"
     CDS             complement(654..860)
                     /locus_tag="ACHLIF_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9609203.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT          285 a          294 c          337 g          273 t
ORIGIN      
        1 caggagcacg tactgatcca tcagatccgt cagtgctgcg ctctgcttct gacggttgat
       61 gatccattcc tccagctctt tttctgattt ccgtttcaga ttctccgtcc agtcgtcaag
      121 gcagtttccg gtcattaaat caagccagca aagccagacc agtttgaggc gcaactccgt
      181 gccaccaaac tgacggaaga ggctgatcag tttatttgcc agcgaacgct gcctcagacg
      241 ttcacattca gacaggagaa tgccttccag catcggatga tgaaccagca gatgttcact
      301 ggtgcataaa ttcactatca gatcatcatc caggggcggc gtggtccgtt tgtccggacc
      361 ataaatcagc cggtgcagac tctggggtac gatggcaatc tccggttttt caccattgaa
      421 tgacacccac ccgggcgtca gattatggaa gtagtagata ttcggatgac acacgcccca
      481 ggatacggcg tgccgggtga agccggtcag ccatttctgg tagctttcgt ggagcagctc
      541 gtaacgggtc tgatactgtg gtttcatttt catattcctc acgggggata acaggcagca
      601 gaacgccgcc ggcaggccgg agactgcgtc tgctgcagat gaacggttaa gaattacgga
      661 cgggactgtg caatacgcgc ctgcagccag gcttccactt cgcttttcag ccatcgggag
      721 ctgcggccca ttttgatggg cgcaggaaag tcaccatcct tgatgagttt gtaaaaccac
      781 ttatcggtca ggccggtcag ttgagtgata aatgccatgt cgaccatctg gtcatccatc
      841 agtgaaactg gggtgatcat gagaatgttc ctccggatgt tgttaaacca gccggtgtgg
      901 tcatgaaaac aacgcaccac cttcaccggc ataacaaccg gggagaaacg taaaaaataa
      961 tcgggaatgt gaccgccagg agataaacgg ggaggttacg gtggctgagc cgtaccggta
     1021 tgccgggtgg ttgttacgaa acgatgcttc cgcacgcgcg cgcgataaag aagggatgat
     1081 tttcgtgagg aagaggggaa aactggcgtt ctgataattg cagagttatt tacggggcgg
     1141 ttacgatggg gcagaccgtg tccggtgggc aggcgacagg tatcaccga
//
LOCUS       DAZGOB010000376         1189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGOB010000376 DAZGOB010000000
VERSION     DAZGOB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.379"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            179..472
                     /gene="iss"
                     /locus_tag="ACHLIF_005432"
     CDS             179..472
                     /gene="iss"
                     /locus_tag="ACHLIF_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO9609204.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(563..745)
                     /gene="rzoD"
                     /locus_tag="ACHLIF_005433"
     CDS             complement(563..745)
                     /gene="rzoD"
                     /locus_tag="ACHLIF_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO9609205.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(962..>1189)
                     /gene="rrrD"
                     /locus_tag="ACHLIF_005434"
     CDS             complement(962..>1189)
                     /gene="rrrD"
                     /locus_tag="ACHLIF_005434"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO9609206.1"
                     /translation="EVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGACDQ
                     LRRWTYAGGKQWKGLMTRREIEREICLWGQQ"
BASE COUNT          302 a          285 c          270 g          332 t
ORIGIN      
        1 ctaaaagggt aatggacaga tagagcggtt tatttcattc cacaggattc tgagtgcccc
       61 cctcctccaa taggctgagc atccacctat atagttttaa ttttcatcaa tccatttaac
      121 tatcgtttaa ttgttgtcac ataggattct gccgttttta acaatgcagg ataataagat
      181 gaaaaaaatg ttgttttctg ccgctctggc aatgcttatt acaggatgtg ctcaacagac
      241 gtttactgtt ggaaacaaac cgacagcagt aacaccaaag gaaaccatca cccatcactt
      301 cttcgtttcg ggaattggac aggagaaaac tgttgatgca gccaaaattt gtggcggcgc
      361 agaaaatgtt gttaaaacag aaacccagca aacattcgta aatggattgc tcggttttat
      421 tactttaggc atttatactc cgctggaagc gcgtgtgtat tgctcacaat aattgcatga
      481 gttgcccatc gatatgggca gctctatctg cactgctcat taatatacgt ctgggttcct
      541 tccagttgtt tttgcatagt gatcagcctc tctctgaggg tgaaataatc ccgttcagcg
      601 gtgtctgcca gtcgggggga ggctgcatta tccacgccgg aggcggtggt ggcttcacgc
      661 actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac atcatcacgc
      721 agagcatcat tttcagcttt cgcatcagct aactccttcg tgtattttgc atcgagcgca
      781 gcaacatcac gctgacgcat ctgcatgtca gtaattgccg cgttcgccag cttcagttct
      841 ctggcatttt tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta atgattaaca
      901 gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc ggttactctg
      961 ttcattgctg accccacaaa cagatttcac gctcaatctc acgacgagtc atgagacctt
     1021 tccattgctt accgccagca tatgtccagc gacgtagctg atcacatgcg cctttgatat
     1081 cgccctggtt tattttgcga agaagcgtcg atgttctgaa attgccagca cccacgttgt
     1141 aaacgaatga gtaaagagcg ccgcgcgttg tttccggtat atcgacttc
//
LOCUS       DAZGOB010000377         1181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGOB010000377 DAZGOB010000000
VERSION     DAZGOB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.380"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1181
                     /locus_tag="ACHLIF_005435"
     CDS             <1..>1181
                     /locus_tag="ACHLIF_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9609207.1"
                     /translation="PRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTAL
                     VGVQVDLEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWC
                     LWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQR
                     KAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSA
                     LKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGL
                     WLIKTELLETQTVDFSVGAEGLRHVPGDVIEIFDDDYAGISTGGRVLAVNSQTRTLTL
                     DREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPT
                     LR"
BASE COUNT          263 a          310 c          376 g          232 t
ORIGIN      
        1 ccgcgcccgt ttaatatccg gatgcgcagg atgacgccgg acagcaccac agaccagctg
       61 cagaacaaaa cgctctggtc gtcatacacc gaaatcatcg atgtgaaaca gtgctacccg
      121 aacacggcac tggtcggcgt acaggtggac ttggagcagt tcggcagcca gcaggtgagt
      181 cgtaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
      241 cgacaataca gcggtatctg ggacggaacg tttaaaccgg catacagcaa caacatggcc
      301 tggtgtctgt gggatatgct gacccatcca cgctacggca tggggaaacg tcttggtgcg
      361 gcggatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtaccg
      421 gacggctttg gcggcacgga gccgcgcatc acctgtaatg catacctgac cacacagcgc
      481 aaggcgtggg atgtgctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
      541 gggcagacgc tgacgttcgt gcaggaccga ccatcagata aggtgtggac ctataaccgc
      601 agtaatgtgg tgatgccgga tgatggcgcg ccgttccgct acagcttcag cgccctgaag
      661 gaccgccata atgccgttga ggtgaactgg attgacccga acaacggctg ggagacggcg
      721 acagagcttg ttgaagatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
      781 gatgcctttg gctgtaccag ccgggggcag gcacaccgcg ccgggctgtg gctgattaaa
      841 acggaactgc tggagacgca gaccgtggat ttcagcgtgg gtgctgaagg gcttcgccat
      901 gtaccgggcg atgttattga aatcttcgat gatgactatg ccggtatcag caccggtggt
      961 cgcgtgctgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     1021 ccatcctccg gtaccacgct gataagcctg gttgacggaa gtggtaatcc ggtcagcgtg
     1081 gaggttcagt ccgtcaccga cggacttaag gtgaaagtga accgggttcc tgacggcgtt
     1141 gcagaataca gtgtgtgggg gctgaagctg ccgacgttgc g
//
LOCUS       DAZGOB010000378         1179 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGOB010000378 DAZGOB010000000
VERSION     DAZGOB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.381"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..704)
                     /locus_tag="ACHLIF_005436"
     CDS             complement(<1..704)
                     /locus_tag="ACHLIF_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609208.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEIKTPIKPPKLGMYEEKYRDYRDKFNEFSAYILQMKLAINKL
                     DSELNKLEPSLSAKNHL"
     gene            complement(856..>1179)
                     /locus_tag="ACHLIF_005437"
     CDS             complement(856..>1179)
                     /locus_tag="ACHLIF_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO9609209.1"
                     /translation="WRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCY
                     DNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFE
                     NKNLA"
BASE COUNT          321 a          268 c          202 g          388 t
ORIGIN      
        1 aggtgatttt tcgctgacaa tgaaggctca agtttattta attcagaatc aagtttatta
       61 attgctaact tcatttgtag aatatacgca gagaactcat taaatttatc tctgtagtct
      121 ctatatttct cttcatacat tccaagtttt ggaggtttga taggcgtttt aatttcactg
      181 cgcaacgcat ctaagtttgt aagatcactg tctataattt taagatagtt taaatctaac
      241 gaaccatcag ttttatcaag cttagttttc agccttttaa cagcctccaa ggaagcatca
      301 agaactggtg tctgattctt caaagcttct gaaaaacttt tttgtgttga ttttcgagct
      361 tgttgttgag cagattttct taactctttt tcacgcttaa cttgttctaa aagctcttta
      421 cggtcatcag aacgtgaacc aaaaaatcta tcagcaagtt cagttaacaa attagatata
      481 atagttttca gttctcttgc agcctggttt cttatgaatc cctctctccc cgacttatct
      541 ttcagctctt tattactgga ttgagtaatt ccaatataac caaaaatcct tctattagac
      601 caataatatc gccctgcatt ccatgaacgt ctttcttcta tctggaagaa atcattatct
      661 actcgaccat aaggtaatac tctcaagcta tccctaaaaa tcattagtcc tgcatacttt
      721 ttggccttaa gatcaaagtg ggaatgttca cgttcagtat gtgatgtatt ttgtgaaagg
      781 aattcaaacg ttcctatctg aagctcaaat gggccgaccc ctgcgtgatc ctacccacgt
      841 aatatggaca caggcctaag cgaggttctt gttttcaaat tgttccggac tgaggccgcc
      901 acaccaactg tgccgccgcc accgattgta atcacattcg atataattaa acaccgttgc
      961 ccgcattatt tcccggctga taaagtgttc tccatggata cattccactt tcagcgaatg
     1021 aaagaagctt tccacgcagg cattatcgta gcagcaacct tttgcgctca tacttccacg
     1081 cagattatgc cgcttcagtt gcgcctgata atctgctgaa cagtactggc ctccacggtc
     1141 cgtgtgaacg ataacgttcc ggggcctctt acgccgcca
//
LOCUS       DAZGOB010000379         1177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGOB010000379 DAZGOB010000000
VERSION     DAZGOB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.382"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            323..583
                     /locus_tag="ACHLIF_005438"
     CDS             323..583
                     /locus_tag="ACHLIF_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO9609210.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEDIPWHDLPASHRGNNQMSLPLPQN"
     gene            774..914
                     /locus_tag="ACHLIF_005439"
     CDS             774..914
                     /locus_tag="ACHLIF_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9609211.1"
                     /translation="MSQKSLTTITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT          354 a          279 c          271 g          273 t
ORIGIN      
        1 aacagaaatg gtgcagacat acattcaaga gcagaagaaa agacagaaaa gttctattag
       61 cgcacaaatc aaaacatgag ggtgtactca aataatacaa tgctatagta aatctataag
      121 tgcataacta tttcctgatc ttttcccaaa atagatgaca ggataaaata cattccaatc
      181 ttttacaggc tttggataaa tcgtatcaaa gcctatttct tcaagagtta aatcaccgct
      241 tttcgctcga aacggactgg ctgttggagt atgagccaga acaacttaga atagcccctg
      301 cactacgtta agggaggtcg ctatgttccg ttgtccgctt tgtggcgcat ctgcccgtat
      361 ccgcaccagt cgtccggaaa atgattcaaa caccgtgcgg caaaagtatt accagtgtaa
      421 caatctggaa tgcggcgtat gcttctcaac actggaagct ttccataaat tcacatcaaa
      481 acacgcctcc ggcgttcact cttcagaaga tatcccgtgg catgatctgc cagcttcaca
      541 caggggaaac aatcagatga gcttgccttt acctcagaat taacaggcag aattgccgga
      601 gtaacaaaaa agcgatagat tacgcgcggg tgcctttcgg ctgatggtcg gagggaatac
      661 ccgaaggcca gatgtggaaa ggccccggaa aacatctctg tttaaccgag gccctaaccg
      721 cattaccttg acaagtgaaa ggttagcgcc tctccggaaa aggagcaagt gctatgtcgc
      781 aaaaatcgct tacgaccatc acgttctgcg tgacggcaat cctcatcatc tggatgttgc
      841 acggttcgct gtgtgaaata cggatgagct tctggggagc ggagtttgcg gcgttcttac
      901 agtgtaagca gtaaggaaac cgcgacgggg gagcaatccc ccgtcaatcg gttgccaggg
      961 taaggccgga taaggcaccc tatctcacag atatgaacaa caaacccgca gcgtaaaaac
     1021 tgcgggtttt ctttttggtg ccctcaccga cttgaaacag tgataagact ataagacaaa
     1081 gcccgcaatg tagcgggctt actcccctca ccctccactg aatgtggaca cacagtggaa
     1141 cgaagcggca taagatatta taaatcaatc agttatt
//
LOCUS       DAZGOB010000380         1164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGOB010000380 DAZGOB010000000
VERSION     DAZGOB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.383"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..896)
                     /locus_tag="ACHLIF_005440"
     CDS             complement(<1..896)
                     /locus_tag="ACHLIF_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609212.1"
                     /translation="MDMIPSYESMYLTLRGIPTDSIGGEDIYFIHDPQGGWYPNRGNH
                     LYAIDALCVNIHDALANSIFDGLENYHKFLYMAPEFLSTAGLNSESVVSKETFVLFID
                     KFKGHTDVNKGLYLFDCCKIVSSIQECSKEVLQLQGEFYYTLNFEPLFFPNIEEEDGI
                     RYVTSPVVTKLFALLGFIYIRMYSLLDYITKLAIEIENLKTQFSSYVKLTSKNSQYGD
                     KKKVSLNNYKGSLFEQCPFINEIESVRNHIIHDGLLDDMPKAYRVIRNNACIEKYLLF
                     PDQTSEGRFESYKSRNLFYGGDT"
BASE COUNT          369 a          218 c          187 g          390 t
ORIGIN      
        1 gtatcaccac cataaaataa atttctactt ttatacgatt caaaacgtcc ttctgatgtt
       61 tggtcaggga aaagcaaata tttttcaata catgcgttgt tccggataac tctgtatgct
      121 tttggcatat cgtctaaaag accatcatgt atgatatggt ttcgtacact ttctatttca
      181 ttaataaatg ggcattgctc aaacaaagat cctttataat tatttaagct cactttcttt
      241 ttatcaccat attgagagtt cttggatgtg agtttaacat aagaagagaa ctgtgttttt
      301 aaattttcaa tttctatagc tagttttgtt atgtagtcga gcaaactgta catgcggata
      361 taaataaatc caagtaatgc aaagagctta gtcacgactg gagatgtaac atatcttata
      421 ccgtcctcct cttcaatgtt agggaaaaac aagggttcaa aattcaacgt gtaataaaac
      481 tccccttgta gttgcaacac ttccttgctg cattcctgta tgctacttac aattttacag
      541 caatcaaaaa ggtaaaggcc tttatttacg tcagtatgcc ctttgaattt atcaataaat
      601 aaaacaaatg tttcttttga aactacggat tcagagttca aacctgctgt tgataaaaac
      661 tcaggggcca tataaaggaa cttgtggtag ttttctaatc catcaaaaat gctattagct
      721 agagcatcat gaatattaac gcataatgca tcgatcgcat aaagatgatt ccctctgttc
      781 gggtaccatc caccttgagg atcatgtata aagtaaatgt cctcacctcc aattgagtca
      841 gttggtatcc ctcttaaagt caaatacatg ctttcatatg aaggtatcat atccatgttt
      901 ttctttaatt cctcttatga atcgaaaaag tgaggtttcg tatataggtt aatttaagca
      961 agataatcat actctgaaag taataatttt tcatatccgg gtttatcaaa cgcagggaat
     1021 tttatgggct tttctttact caacctaacg cccgcttctg gcacagagcg gactgtcaga
     1081 ttagattcag acctgtactc attagctgat acaaatcagc cacatatacc tgcattccaa
     1141 ataatttcag acagatatta tcgt
//
LOCUS       DAZGOB010000381         1161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGOB010000381 DAZGOB010000000
VERSION     DAZGOB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.384"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..161)
                     /locus_tag="ACHLIF_005441"
     CDS             complement(<1..161)
                     /locus_tag="ACHLIF_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9609213.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALA"
     gene            complement(142..555)
                     /locus_tag="ACHLIF_005442"
     CDS             complement(142..555)
                     /locus_tag="ACHLIF_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9609214.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGIYGGVRYVPASG"
     gene            complement(582..1004)
                     /locus_tag="ACHLIF_005443"
     CDS             complement(582..1004)
                     /locus_tag="ACHLIF_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9609215.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHY
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            complement(1018..>1161)
                     /locus_tag="ACHLIF_005444"
     CDS             complement(1018..>1161)
                     /locus_tag="ACHLIF_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO9609216.1"
                     /translation="KATISVKDMTITVNGVATGKVQIPVVSGNGQFAAVAEVTVTEAG
                     AAG"
BASE COUNT          261 a          356 c          263 g          281 t
ORIGIN      
        1 gccagcgcct gtcggctcag cccctgttca acagtactgg cggtttttct ggcatccgcc
       61 tccagactgg aaaaatgacg gcgtacccgg gccatctgtt catcaaaccg ggccgcatcc
      121 agactcaaat caataaccag atcacccgct ggctgggaca tatctcacac ctccgtaaat
      181 ccccgctgaa gccatcatta atgcaacatc atcctcgctg acatccacca catccgcaga
      241 aggtgaaata tcgcgccctc cgtcccctcc gaaccggacg cctccggcaa ctcctgtcgc
      301 tttctgcatc agcatttctt cctcgtccgg catctccgtc tgctcttcct cacacgctgg
      361 agcaagcaga ctgaaatccg ccggatgcat atccggatcg ccaaaaaaca ggctgagtac
      421 ggcgtacatc agcccggaaa aatgagcgtc cagttgggta tcctgaaaat aatgcgtgca
      481 gtaaaaacgt cgccagtcgg catattcggt ggatgtcatc ccggcaagca tggcgcgcca
      541 gtcgggtctc cccatctctc gcgccagttt caggacaaag ttcagctcgc cttcgaatgc
      601 ttttttgatg ttaccggctc agtcgcttct gctttcccgg tttgttcagg atcggcatcg
      661 tgccggttat ccagcatacc tgaaagataa agcacccggt tcgttgcctg attcagtgca
      721 tcagcaggcc atcccagcat cacttcacgg cggatctgct gcatctctgt ctccggagag
      781 gccagagtgc ctttgaggga atgggaatgc catagcgaca tcgccacaag cagggatgcc
      841 gtttccagat atcgctggtt aatgtgcacg acataatgct tcgttgtctc ctgttgttct
      901 gcgtctgaaa caaactttat ataatcaaac cgctgcagcg cagacaattc ggaaagcgtg
      961 acggacacac cgttatattc aaattgttct gttttcagaa acatgtatta cctccgttta
     1021 ccctgcagcg cccgcttcag taacggtgac ttcagccact gcggcgaact gaccatttcc
     1081 gctcaccaca gggatctgca ccttacctgt cgccacgccg tttaccgtaa ttgtcatatc
     1141 tttcacacta atggtggctt t
//
LOCUS       DAZGOB010000382         1149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGOB010000382 DAZGOB010000000
VERSION     DAZGOB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.385"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1149
                     /locus_tag="ACHLIF_005445"
     CDS             <1..>1149
                     /locus_tag="ACHLIF_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO9609217.1"
                     /translation="YKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISDSFGGPL
                     SLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAAGGGVAV
                     LAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIRQAAENL
                     ARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYN
                     FLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIWDDVKTW
                     TSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETTKNVPGV
                     GALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRA"
BASE COUNT          265 a          271 c          382 g          231 t
ORIGIN      
        1 gtataaggct gccatgcgca cactgcccgc acagttcacg gatattgtca ctcagcttgc
       61 cggtggtcag aatcccttcc ttatcatgct gcagcagggg gggcagatca gcgattcatt
      121 cggtggaccg ctcagcctgc ttaccctgct gaaggaggaa cttctcggga tcagggatgc
      181 ctctgaatca tcagaggagt cgctgtcaga tacggcaaat gcactggctg aaaatgcccg
      241 gaatgccggt gagctgggac gatttatgtc ggtggcccgt gtggcggcag gtggcggggt
      301 tgccgtactg gccgcgcttg ctgccgccgc ctggcaggca gagcaggctg accgggcctt
      361 attgcgttca ctgatcctga ccggaggggc ggctgccacc acaacggcag aattgtggaa
      421 aatggccggg gtgatcagcg atgaagccgg tggtggtatc agacaggcgg cagaaaatct
      481 ggcccgtctg gcagaaagcg ggaaatatac cgccgggcag ctacggatca tgggggaaac
      541 ctctcagaga tggctgcaga cggtggggga cgatgccggg aaggtggaaa aagcctttga
      601 agggattgca gcagatccgg tgaaggcgct ggcctccctg aatcagcagt ataacttcct
      661 gagcgtttcc cagttacgcc atattgatga gcttgagcgc acgaaaggta aacaggctgc
      721 ggtgacggag gcgatgtccc tgtttgcgga tgtcatgaat gcacgtctgg agcaacttga
      781 taaagcggcc acgccggtgg aaaaaatctg ggacgatgtt aaaacctgga cttctgacgc
      841 atgggcatgg ataggtgatc atacactggg ggcactcagt ctgatcactg acgtggtggc
      901 cggaaccgtt gaacaagtga agctgctgct tgtgcagggg gatctggcgc tggctgaatt
      961 tattcagtca gcctgggaaa cgacaaagaa tgtgcccggc gttggtgcgt tgtttggtga
     1021 actggcagaa gagaaccgcg tatttattga gaaaacaaaa cgcgatgaac tggcgctgag
     1081 aaaatccatt gcggaacggg atgcgcgtat acgccagggg gaaatggggt acatcaaccg
     1141 ctcgcgtgc
//
LOCUS       DAZGOB010000383         1145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGOB010000383 DAZGOB010000000
VERSION     DAZGOB010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.386"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(33..>1145)
                     /locus_tag="ACHLIF_005446"
     CDS             complement(33..>1145)
                     /locus_tag="ACHLIF_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO9609218.1"
                     /translation="LIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVYTTTQSNEPP
                     AGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNLGYSV
                     DEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSLDD
                     ILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVVRRKSEASRF
                     QDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQILDSLAEAGIP
                     DESVVGISQGWKLGGAIKTTERKLAEGVLIHGDQPLMAWCVGNARVEPKGNAILITKQ
                     ASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT          250 a          382 c          268 g          245 t
ORIGIN      
        1 agcggttcgt gatggttaca gtgtgaacag ggttatatga aaaaaaccgc atattctttc
       61 tttttcggtt ccgggttaag ggacatcagg gagaccgcat tgaacagcgc catcagcggg
      121 tcaatttttc cccgtccgct ggcctgtttg gtgataagaa tggcgttacc tttaggctcc
      181 acccgggcat taccgacaca ccaggccatc aggggctgat caccgtgaat cagcacccct
      241 tcagccagtt tgcgctcggt ggttttgatg gccccgccca gcttccagcc ctggcttatc
      301 cccaccacac tctcatcggg gatcccggct tccgccagtg aatccagaat ctgccccaca
      361 cctgacgggt caataccgat atgctccagt aactcagcct catgaatacg acgcacatac
      421 tccgccactt ccgccgtgtc atccccgacc cgacggacaa tcgtcatgtc tccacaggcc
      481 acaaaatcct gaaaacggga tgcctcactc ttccgtctga ccaccgcggt ttcatgcgcc
      541 caggcatggc cccagcccag ccattcgcgg gtttccctgt cacggccaat cacgtacatt
      601 cccagcagat catccaggcc cccgccgtca atccccaccg tcaccacatc agcgcgctgc
      661 aggatatcgt ccaggctgac gcgcctgccc tgctgctccc agaaatccgc acccgcccag
      721 cggtcagaac gcagggcaag accaatttcc acattggcat gttttgacat gaagccacga
      781 aatgcttcct caccagcctc ccgggcttta cggtactccc ggtacagaaa agcctcatcc
      841 accgaataac cgagattcgg attgaccatg gcgaggtttt ccatcagcag gtgagccccg
      901 ctttccacca tttcaggagg gtgttcaaat atcaccggca gaaaatgcgg atcatgaatt
      961 ttgccgtcac ggacatcccg ggcgtactgc agtttctgtc tgaacacccc ggctggcggc
     1021 tcattcgact gggtggtcgt atacaccaca aacccttccg gacgggaggc aagcccgccg
     1081 atggcttcac gtagcatgtc ttccgccttg tactgcttgc caaacagcca cagttcatca
     1141 atcag
//
LOCUS       DAZGOB010000384         1143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGOB010000384 DAZGOB010000000
VERSION     DAZGOB010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.387"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..808
                     /locus_tag="ACHLIF_005447"
     CDS             <1..808
                     /locus_tag="ACHLIF_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO9609219.1"
                     /translation="RMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGK
                     RFINGAVIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQ
                     DLSPSGPLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEY
                     KKLFALPDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNAL
                     LNILESQFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
BASE COUNT          305 a          251 c          287 g          300 t
ORIGIN      
        1 cagaatgacg ttatccctca tggtgcaacc cggtcttttt gaccgatata tggagcgaaa
       61 aggttctgtt gccagggaca gtggttttct tgcccgttgt cttatcagta aacctgcaac
      121 cacgcagggg aaacgcttta ttaacggagc tgtgattccg ggaggatcat taacggcatt
      181 tcatgaacga ttaatggaac tggccagagg cagtattgaa aaatccagtg aggatgaacg
      241 gtattgtctg catttttcgc ctgaagcgca aaaaatattt attgagcact ataatgttct
      301 ggagcaggat ttatcccctt ccggaccctt aagcccgttt cggggacatg tttcaaaaaa
      361 aacagaaaac attgcaagaa ttgcagcgtt atttcagtat ttcagctatg gtgaagggaa
      421 aatctctgcc gatatcatga cgtctgctgt cgtaataagt tcgtggtaca cagatgaata
      481 taaaaaatta tttgcgttac ctgatgaatc tgaactgcag cagaaggacg ccgaggagct
      541 gttcgactgg ctcattgagg agtgccgcgg agaatgcccg ccccgtgtca ggaaaaatta
      601 tattctccag tgtggcccgg gccggttcag aaacaggaaa aaactgaacg cattgctgaa
      661 cattcttgaa tctcagttca ggctttctgt cgtgccggaa ggaaaaacca tgtatgttct
      721 gcttccccag atagccagtt taaagttgtc tgatgtctct ggtattttta cctcgggtta
      781 tcactacaat aagctgagag cgaagtaacc ggtgagcagt ttttctccat tatgcttcag
      841 acttagatga ttgattatta tggggatttc atcaccgata ttcagatgta tgaatatcgg
      901 tgatgaacta aagctcagga gctgcgtggt ggtgataaat caacaggcat ataactgata
      961 cccgccggtc agaagttcca gcctgcgact ttcctgtctg cgtgcccgca gtccggcgtt
     1021 caggtgcagc gtgtgacaga acgcacggag agcgaaacgg caggcagaca ggccgttcag
     1081 tcagccctgg caccctgccc ggataccagc ttccctgaaa caactggcat caggtgcgct
     1141 tat
//
LOCUS       DAZGOB010000385         1137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGOB010000385 DAZGOB010000000
VERSION     DAZGOB010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.388"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..272)
                     /gene="ybfB"
                     /locus_tag="ACHLIF_005448"
     CDS             complement(<1..272)
                     /gene="ybfB"
                     /locus_tag="ACHLIF_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO9609220.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFL"
     gene            complement(272..>1137)
                     /locus_tag="ACHLIF_005449"
     CDS             complement(272..>1137)
                     /locus_tag="ACHLIF_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9609221.1"
                     /translation="VSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLP
                     LALISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDP
                     LQGRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMGGTGERSGPDI
                     WGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVETD
                     NSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNIG
                     RFIRFVFTGVM"
BASE COUNT          311 a          292 c          215 g          319 t
ORIGIN      
        1 agaaaataaa gccatattat tatcgccata atatacttac tatcaatcct tacgccaata
       61 atgtctgata cagtccctat aaatattaga gcaataaaga atactatgcc tgttggaaaa
      121 catattacca tcattccata agacatgaga ctaattaact cgctcacatc acgagtggag
      181 tctaatagtg aaagtctatg catggaaaag aatagtatta aaagcgataa agccagccat
      241 atagctctga ataaaaaaat aatatatttc attacatcac tccggtgaat acaaatctaa
      301 taaaacgtcc aatgttattt ccggagcact tcatcttaca tgtttctaaa gaatcattag
      361 accagtcttg gcagttttta aatagcccta aatttatagg tgtgaatata tcaggcaata
      421 cagaataacg cggccttacc tctttaaact tcccttttaa acagttctcc acacaactat
      481 tatcggtttc aacgaggtca caacggccag cagcttcttt tgtatcatta cttgctttac
      541 ctggccccca tagaccatct ccttttgatt ctccccgttg gtcttggcca ccacaagtat
      601 agtccccttt accatctggg acacaaagat attgatgata gaacggattc ccccatatat
      661 ctggaccgct tctttcacca gttccgccca ttgaatgcag tggcttaatg cattttagtg
      721 tttctaatcc taatggatct atatttgaga tcggattcaa cggatactga taaaaattcc
      781 atcccccctt cagcccaatc ggatcctgag tgatataccg cccctgcagc gggtcataat
      841 agcggtggcg gttgtaatac aggccggact cctcatcata ctgctgcccc ggcagccgga
      901 taagctgctg cagctgatgc gggttctctt cattcagcag gttgccccat tcatcatatt
      961 ctgcgcacca cgctgttgcc ccttccgtgc tgatgagcgc cagcggcagg ccgcgatggt
     1021 cgcagtggta caggtggatt tttcgcgccg gcgtgtacac cgggtccatc tggttttgca
     1081 tctgctccac ggtcaggccg cacgatgcca gccagcggcg gctttcctca ctcaccc
//
LOCUS       DAZGOB010000386         1130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGOB010000386 DAZGOB010000000
VERSION     DAZGOB010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.389"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..393)
                     /locus_tag="ACHLIF_005450"
     CDS             complement(<1..393)
                     /locus_tag="ACHLIF_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO9609222.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKN"
     gene            complement(1028..>1130)
                     /locus_tag="ACHLIF_005451"
     CDS             complement(1028..>1130)
                     /locus_tag="ACHLIF_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO9609223.1"
                     /translation="EFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          323 a          235 c          193 g          379 t
ORIGIN      
        1 attcttatct tttattttat ttactgtttc aatgcaatca gcggcaagtt ggagtggatt
       61 tagatttttc agatatggtc ttatgatctt tcccttactt aataaatagc atgtatagga
      121 ttcggaaagt ccagcatcta ttttgtacct cattatatta gataaggatg tgagaaattc
      181 cttgttatca ttcgcagcca tatatgccag tccgagttca gcctctgcgg ccttacaggc
      241 aaaatagctt agtgaacacg cttttcgtcc ctctttagta ctggctgata ttagtgccca
      301 taatattgct ttgtctgcca aatctaactc tctctgaaat tgtcttgcag taactgcact
      361 cgcagaaata gagaaaaaaa tacaattgtt aataaccttg ccatattttt tagtccgtaa
      421 ctgttaatgt gctatctggc tctgaaaata ttaactcatt taatttttgc atgtcattag
      481 ataaaccagt tatacaaccc tcggaacaga atcgaggtcc taaagacgga ccaaaagcgt
      541 ttaaacaact atgtattgaa aatccattgc gattataagt tcctgtatta actgacggta
      601 ctaatcgtat gccgtcaggt tttctattgc ttgctcccgg gtcattaaca ttctaactcc
      661 ataggccttg aggtattggc catctattta taaatgtgta caacggggat tatttttaca
      721 ttgcatatta ttaccagaag cgactggaat actaagcaca tcataaaaat tatcatcaca
      781 tggtatatct gaaacacacg aaagcatacc taaagttata ctgtataaac aggtggctaa
      841 tcccaagggg atcgattcca gaaatcgggt tcaacggata ctgataaaaa ttccacctac
      901 cttccagccc aatcggagcc tgagtgttat accgcccctg ctgcggttaa tgttaccgat
      961 aatagttata gtacagccct gattcatcat catactactg ctgcggcagt ctgatgaact
     1021 attgctttta cggattctcc ttattcttcc ttctatccat tcgctttagc tttttgagct
     1081 cttgaagttt gctctttgca tcaatattac cgttatttac agcgaattcc
//
LOCUS       DAZGOB010000387         1128 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGOB010000387 DAZGOB010000000
VERSION     DAZGOB010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.390"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            67..1128
                     /locus_tag="ACHLIF_005452"
                     /pseudo
     CDS             67..1128
                     /locus_tag="ACHLIF_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          440 a          217 c          161 g          310 t
ORIGIN      
        1 ccattcctgt tctaattatc caatttaaac atcttagcat aaaacaaatg atgaataagg
       61 aattttatgt caattgactt taccccaggt ataataaata catatcacgg cgatatttat
      121 aactgcacaa caaataccga taatgctaaa acgccagaca caccgaagtg gccttgcgat
      181 aattgggaag aacaataacc cattaattcc acattctctg gagaaggata tatctctgat
      241 caatatgatt tagcgcaaca ccaacttcaa caaattaatg cgtgccacac gaacactaca
      301 tatacaaatg cagactactc aaaagttgtg gctcaacttg taagtcttat tactaacatt
      361 gaaacgataa gttcgacaca acttacgcaa cagacacaat caatacttaa tcagattaat
      421 aacattagat atgaaaaaaa caaaagtgct gaatgtcgaa tcattgttgt cgctaatcct
      481 aaacccgaca aggcaatcat aactaaaatt agcgtagaag agggaatacc cataacattc
      541 agtgtccaaa ccatgttttc agatactaat tttattgctg aacaacgagc tgacttacca
      601 actaatataa aagatattca aagcctgtat caaaaaatga cgaagttata tattgagcac
      661 agtgaaaata aaaacaggat gaaagtcttt gccggaacca actttattga tttcaatatg
      721 acaggacaga acctgtccgg gttcgttcta accttgtcaa ggttttattt cgaagatcta
      781 cttaatataa atttcactga cgaaaattta ggtgacacta ttttttacat aaagaacacc
      841 ctacccccaa attatataaa gatggacaat atcttgacaa acaaatcgaa ggtttatttt
      901 caacattatt aaccattaat gacaactttt tacgagcaaa agcggaaatt gcttcgacaa
      961 taattaaatt tttagaggcc aggataacca atctttcata caatgacata ttaacatatc
     1021 agcaagaatt ccaaaaacaa tgttataagc aagttaaagc ctttacgaca ctctctcgat
     1081 acaataaaat tcaaacatgg gcagaaatgt ctgaatatca gttcgaag
//
LOCUS       DAZGOB010000388         1127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGOB010000388 DAZGOB010000000
VERSION     DAZGOB010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.391"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1127
                     /locus_tag="ACHLIF_005453"
     CDS             <1..>1127
                     /locus_tag="ACHLIF_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609224.1"
                     /translation="SSKVIIEISLSTTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFER
                     KDDSSLHLITNKVLACYQREANKNMALLLNNHQLNWKDAIDYNELLEKPLASTLEKIK
                     KEHLLLMPHVCDDTIPYLLGEGGILEEINDLKALHDTRIASGTEGNDEINNIKINLSN
                     ILIDSLDDAKVNLEKVIDSMLKTFLKLPYINDVKILEWCFNQSMQYSDDTTKIKHARS
                     VIDHIDFNCDQSKIAETLFFNLNKEPYKNSPELQELIWEKLVVYVNDFNLSNQEKSRL
                     ILRLFDDVKLLFNEVPVSILVNDIFLKDFFMKQPDFAKWYFYQLIKKYEGEQLYLNEL
                     GYVYGNEEKTNEIVKKLPGYVIKIFEEKMGNELKIRTRMMK"
BASE COUNT          407 a          152 c          209 g          359 t
ORIGIN      
        1 attcatctaa ggtgattata gaaatttcac tttctactac cactactatg gggactcgtc
       61 aaggacatac agccattata tttccacaat ctgatttttt gagttttcgt tatgaaggta
      121 agtcttttga aagaaaagat gatagttcat tacatctgat tactaacaag gttctggcgt
      181 gttaccaacg cgaagctaac aagaatatgg cgcttctatt aaataatcat cagttgaact
      241 ggaaggacgc aattgattat aacgagttat tagaaaagcc attagccagt acattagaaa
      301 aaataaagaa agaacatctg ctattaatgc ctcatgtatg tgatgacacg attccatact
      361 tactaggcga aggtggtata cttgaagaaa taaatgatct gaaagcgtta catgatacca
      421 gaattgctag tggtacagaa ggtaatgatg aaattaataa catcaaaatt aacctgtcta
      481 atattcttat tgattcctta gatgatgcaa aagttaatct cgaaaaggtc atcgattcga
      541 tgttgaagac ttttttaaaa ttgccatata ttaatgatgt aaaaattctg gagtggtgtt
      601 ttaatcaaag tatgcaatat tctgatgata ctacaaagat aaagcatgcc cgctccgtga
      661 tcgatcatat tgattttaac tgcgatcagt ctaaaatagc tgagacatta tttttcaacc
      721 tcaataaaga accctataaa aacagccctg agttacagga attgatttgg gagaagttgg
      781 ttgtatatgt caatgatttt aacttaagca atcaagaaaa atcacgctta atactaaggt
      841 tatttgatga tgttaagttg ctatttaatg aagtgcctgt cagcatttta gtaaatgata
      901 tttttttgaa ggattttttc atgaagcaac ctgattttgc gaagtggtac ttctatcaat
      961 taattaagaa gtatgagggc gaacagctgt atcttaatga gttaggatat gtttatggta
     1021 atgaagaaaa aacaaatgaa attgtaaaaa aacttccggg ttatgtcatt aagatttttg
     1081 aagaaaagat gggtaatgaa ttaaaaatta gaacaagaat gatgaaa
//
LOCUS       DAZGOB010000389         1122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGOB010000389 DAZGOB010000000
VERSION     DAZGOB010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.392"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..192
                     /locus_tag="ACHLIF_005454"
                     /pseudo
     CDS             1..192
                     /locus_tag="ACHLIF_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            321..566
                     /locus_tag="ACHLIF_005455"
     CDS             321..566
                     /locus_tag="ACHLIF_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609225.1"
                     /translation="MDIRHSLNEWWTLWQASRAAIELDIDWPESNDDFWKDGEEGAYA
                     MGYEDGRDKTVIAVMKAIRAAGIKEKNFDEANNIPPN"
     gene            538..948
                     /locus_tag="ACHLIF_005456"
     CDS             538..948
                     /locus_tag="ACHLIF_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO9609226.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            945..1121
                     /locus_tag="ACHLIF_005457"
     CDS             945..1121
                     /locus_tag="ACHLIF_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO9609227.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
BASE COUNT          339 a          229 c          291 g          263 t
ORIGIN      
        1 ctcgtcgcac tcaaatgcgt tggcgtttga tggattcttc tgacgttatt tattgttctg
       61 catagctaca ttcgtcttgt gaatgacggt aaagacccat ggtatacgtt gtttggcgct
      121 gcatttgtct gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat ggcgtggcgt
      181 tttgtgagtt gaactgaggg taagtatcga tggacgaatc aagaaagcag tttgaggaat
      241 acgttgccaa aaaattgaga ttaccattcg agatgataac cgaggcaaga aatggtgata
      301 ggtacttcgc attttcaagc atggatattc gtcactcctt aaatgagtgg tggactttat
      361 ggcaggcatc gcgagcagct attgaactgg atatcgactg gccagaatcg aatgacgact
      421 tttggaaaga tggtgaagaa ggtgcttatg cgatgggtta tgaggatggg cgtgacaaaa
      481 cggtaattgc agtaatgaaa gctatcagag ccgctggaat taaagagaag aatttcgatg
      541 aagcaaacaa tattcctccg aactaagcaa caacagcaag ccgcaatcaa cgccatcctc
      601 gcaacaccac tcgataaaga caagccagtc accatccgca ttactgacta caagcgcaac
      661 cttgaccaga acgcaaaatt tcacgcgatg ctggcggata tcgcacgtca ggttcaatgg
      721 tgcgataagt ggttaaaacc agaacaatgg aaggttttgt tgattagcgg tcatgcagtg
      781 gcaacaaagc aggaagctga tgttttgccc ggccttgaag gcgaatacgt caacattcgc
      841 gaaagtagcg cgcagatgag tgtgaagcgt atggcaagtc tgattgagta cacgacagca
      901 tgggctattg gtcagggcgt cagatttacc gacaggaggt acgaatgaga cgacagcgac
      961 gaagtatcac cgacataatc tgtgaaaact gcaaatacct tccaacgaaa cgctccagaa
     1021 ataaacgcaa gccaatccca aaagaatctg acgtaaaaac cttcaactac acggctcacc
     1081 tgtgggatat ccggtggcta agacatcgtg cgaggaaatg ac
//
LOCUS       DAZGOB010000390         1122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGOB010000390 DAZGOB010000000
VERSION     DAZGOB010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.393"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            358..546
                     /gene="dicB"
                     /locus_tag="ACHLIF_005458"
     CDS             358..546
                     /gene="dicB"
                     /locus_tag="ACHLIF_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO9609228.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            543..731
                     /locus_tag="ACHLIF_005459"
     CDS             543..731
                     /locus_tag="ACHLIF_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO9609229.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPLKKVSLYLNNETPAG"
     gene            827..>1122
                     /locus_tag="ACHLIF_005460"
     CDS             827..>1122
                     /locus_tag="ACHLIF_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9609230.1"
                     /translation="MSKVFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETES"
BASE COUNT          296 a          245 c          268 g          313 t
ORIGIN      
        1 tgtggcaatc cgcgaaatgg cgcggcggta agtatggcgg ggttattcct tcccccgttg
       61 aggacaccgg gttgtcaggt tgaccatacg cttaagtgac aactccgctg caacgccctc
      121 tgttatcaat tttctggtga cttttggcgg tatcagtttt actccgtgac tgctctgccg
      181 ccctttttaa agtgaatttt gtgatgtggt gaatgcggct gagcgcacgc ggaacagtta
      241 aaaccaaaaa cagtgttatg ggtggattct ctttatccgg cgttaattgt taactggtta
      301 acgtcacctg gaggcaccag gcactgcatc acaaaattca ttgttgagga cgcgataatg
      361 gaaacgttat taccaaacgt taatacgtct gaaggttgtt ttgatattgg tgttctgctc
      421 agtaaccggg agtttactga agatgccatt aatatgagga aatatgagcc ttatctgctc
      481 aatgataatt ccatactttc ccgaattgct cttcttgaac ttggtatttt cggagaacgt
      541 caatgacttc agcatttgca ctgatgatga cggtttttct tataacgggt gaatcacaga
      601 atgtgattac cggaatttat gcaagtaaag aatcctgcct ccaggcaaga gacgagcaaa
      661 aaatttctgg tgaatgcctc ccgctaaaaa aagtatcgct gtacctgaat aacgaaacac
      721 cggctggata atcctccagc catattaaca ccataccaac ggattaaaaa tgccagcaat
      781 ggcagggatt cgttcaccct gaaatctgta atgaggttaa aacaaaatga gtaaggtctt
      841 tatttgcgct gctattcctg atgaacaggc cataaaagaa gatagcgctg ttgcggtggc
      901 cactgccatt gaagctggtg atgagcgtcg cgcacgcgca aaatttcatt ggcaatttct
      961 ggagcaattc cctgcagctc aggactgcgc ttataaattt attgtctgtg aggataaacc
     1021 cggcataccc cgccctgccc tcgattcctg ggatgctgaa tatatgcagg aaaaccgctg
     1081 ggatgaggcg tctgcttcct ttgtcccggt tgagactgaa tc
//
LOCUS       DAZGOB010000391         1121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGOB010000391 DAZGOB010000000
VERSION     DAZGOB010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.394"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1121
                     /gene="rhsB"
                     /locus_tag="ACHLIF_005461"
     CDS             <1..>1121
                     /gene="rhsB"
                     /locus_tag="ACHLIF_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO9609231.1"
                     /translation="VYDRSNTQVRSFTYDDKYRGRMVAHRHTSRPEIRYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLE
                     IRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYAQLLSF
                     TDCSGYVTRYDHDRFGQMTAVHREEGLSQYHAYDSRGQLIAVKDTQGHETRYEYNIAG
                     DLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRY
                     DVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHW"
BASE COUNT          283 a          330 c          334 g          174 t
ORIGIN      
        1 agtgtatgac cgcagcaata cacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgagccg accggaaatc cgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagc tacacgtatc agtatgagaa
      181 agaccacatc accatcaccg acagcctgga ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg gcagggcatc
      421 ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg acgggctgga
      481 aatacgccgg gaatatgatg aattgggccg tctgattcag gaaactgccc ctgacggcga
      541 tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa cggaagatgc
      601 caccggcagc cggaaaacca tgacgtggag ccgttacgct cagttgctga gcttcaccga
      661 ctgttccggt tatgtaaccc gttatgacca tgaccgcttc gggcagatga cggcggtgca
      721 ccgcgaggaa ggcctgagcc agtaccacgc atacgacagc cgtggacagt taattgccgt
      781 gaaagacacg cagggccatg aaacgcggta tgaatacaac atcgccggtg acctgaccgc
      841 cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg gaaaggccgt
      901 ccgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg gacgggtcat
      961 ccgcctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac tcgaccggct
     1021 gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc tgaccggcaa
     1081 acttatccgc agcgaggatg aggggctggt cacccactgg c
//
LOCUS       DAZGOB010000392         1118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGOB010000392 DAZGOB010000000
VERSION     DAZGOB010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.395"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1118
                     /locus_tag="ACHLIF_005462"
     CDS             <1..>1118
                     /locus_tag="ACHLIF_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609232.1"
                     /translation="AIGEGPIEGPVKGLQSILVNKTPLTDTDGNPVIHGVTAVWRAGE
                     QEQTPPEGFESSGAETGLGVEVTKAKPVTRTITSANIDRLRVTFGVQSLLETTSKGDR
                     NPSSVRLLIQLQRNGNWVTEKDVTINGKTTSQFLASVILDNLPPRPFNIRMVRETADS
                     TSDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVP
                     SNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAI
                     AQYCDQTVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQD
                     RPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEV"
BASE COUNT          248 a          307 c          362 g          201 t
ORIGIN      
        1 gccattggtg aagggccgat tgaaggcccg gtgaaggggc tgcagagtat cctggtgaac
       61 aaaaccccgc tgacggacac ggacggcaat cctgtgatac acggtgtgac cgcggtctgg
      121 cgcgccgggg agcaggagca gacaccaccg gaaggctttg agtcctccgg agcggaaacc
      181 ggactgggcg tggaagtgac gaaggcaaag ccggtaacgc gcaccattac atccgcgaac
      241 attgaccgcc tgcgggtcac cttcggggtg cagtcactgt tggagaccac ctcaaagggc
      301 gaccgtaatc cctcttctgt ccgactgctg attcagttgc agcgtaacgg taactgggtg
      361 acggaaaagg atgtcaccat taacggcaag accacctcgc agtttctggc gtcggtgatt
      421 ctggataatc tgcctccccg cccctttaac atccggatgg tcagggagac ggcggacagc
      481 acctcggacc agctgcagaa cagaacgctg tggtcgtcat acaccgaaat catcgatgtg
      541 aaacagtgct acccgaacac ggcgattgtg gggctgcagg tggatgcgga gcagttcggc
      601 ggtcagcaga tgacggtgaa ctaccatatc cgcggtcgca tcatccaggt gccgtcaaac
      661 tacgacccgg aaaaacgcac ttacagcggt atctgggacg gcagcctgaa accggcatac
      721 agcaacaacc cggcctggtg cctgtgggac atgctgactc acccgcgcta cggcatggga
      781 aaacgtctgg gggcggcaga cgtggacaaa tgggcgctgt atgccattgc gcagtactgc
      841 gaccagacgg tcccggatgg cttcgggggc acagagccgc ggatgacctt taatgcatac
      901 ctgtcacaac agcgtaaggc gtgggacgtt ctcagtgatt tctgctcggc gatgcgctgt
      961 atgccggtat ggaacggcca gacgctgacg ttcgttcagg accgcccgtc ggatgtggtg
     1021 tggccgtaca ccaacagcga tgtggtggtg gatgataacg gcgtggggtt ccgctacagc
     1081 ttcagtgccc tgaaggaccg gcacacggcg gtggaggt
//
LOCUS       DAZGOB010000393         1112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGOB010000393 DAZGOB010000000
VERSION     DAZGOB010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.396"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            306..626
                     /locus_tag="ACHLIF_005463"
                     /pseudo
     CDS             306..626
                     /locus_tag="ACHLIF_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            838..988
                     /locus_tag="ACHLIF_005464"
                     /pseudo
     CDS             838..988
                     /locus_tag="ACHLIF_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          313 a          245 c          265 g          289 t
ORIGIN      
        1 gaacctttta tgcaggcccc gggtttatct gtggttgctg gttaaagcca gtattccgtg
       61 tctgatatca acagcatgcc cttcggatat atgttacttt tgcgacttat gaaggaacgg
      121 gccctggaaa ataagatacc cggtcaatga aaattcaaat tcaacaggta tagaaacttc
      181 agcgttatga aaaatggtgt cataccatat ttacgcctca ccgaatatcc agcgtccaga
      241 actaagccta gggaacatat ataaacgctg cgcattaata ataatcaaaa atcaggagag
      301 ttaatatgaa tttacaatcc ggacaaaaca taccccttca gcaatctacg atcaggctga
      361 atcttcagta cccagccaaa tccggcttta aaggcgaacc cgatacctgc ctgttcatgc
      421 ttaatgctca gggaaaggtc agtggcgatt ctgactttat cttttataat aatttgtctt
      481 ctcctgaggg ggcagtcagg ctcgttaccg gttcgcagca ggccagcatt gagatagcac
      541 tggatcgtgt tccggcgaac gtcagtaaaa ttgcgatcac cgttgtcatt gatggtgaag
      601 ataccatcag ttcaggctgt acgcttactg acaaaatggc gattgagaag gtgcaattga
      661 cttatttgga tttggtcgtg gcgaggtggt gaccctgcat tctgctaacc gggttatgag
      721 tcaggagtca gcgatagaca aagggactta tctgaccggg cccctggaaa aatggcggga
      781 gagaatgata ccttcctgct gacgagaccg gactttgaga tgacgagcga tagtgcggca
      841 gtgatgtaca gcctgatcgg tagctgcaaa acttaacgga atcgagccgg aaacgtggct
      901 acgccacgtg atcagtgtaa tcaacacctg gcctgccaac cgcatgaaag agttgttgcc
      961 ctggaacgtc actctctctg taaactaatt tttaccccac gtccttcacg gtgcgcttac
     1021 actctggaca agtatgtaaa tgataagtta aaaataatcc gtgcatttcc tgtgacgata
     1081 ttagttgttg gtaacgaaat ctcattagcg ga
//
LOCUS       DAZGOB010000394         1109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGOB010000394 DAZGOB010000000
VERSION     DAZGOB010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.397"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..150
                     /locus_tag="ACHLIF_005465"
     CDS             <1..150
                     /locus_tag="ACHLIF_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9609233.1"
                     /translation="DENGIIPEQDRVITQVVILDTDKKLIQCVVRPLQILRADGTWEN
                     IGGMK"
     gene            256..>1109
                     /gene="nleH"
                     /locus_tag="ACHLIF_005466"
     CDS             256..>1109
                     /gene="nleH"
                     /locus_tag="ACHLIF_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HFO9609234.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDRTRNEFNPIDISSYNISERSWSENQIMQ
                     SYHGGKQDL"
BASE COUNT          340 a          178 c          278 g          313 t
ORIGIN      
        1 gatgagaatg gcattattcc ggagcaggac agggtaatca cacaggtggt gatactggat
       61 acagataaaa agctgataca gtgtgtggta agaccgctgc aaatcctgcg tgctgacggg
      121 acgtgggaaa atattggcgg gatgaagtaa cccgacagct tcacaaaacc ggagtccggc
      181 tccggttttt gttgtcatgt catggtgatg tttgttaaga aagtaaagat tgattcattt
      241 tgaaggttga aatgtatgct atcaccatct tctgtaaatt tggggtgttc atggaattct
      301 ttaaccagaa acctgacttc tcctgacagt cgtattttat cctctgtaag ggatgcagct
      361 gctagctcgg ataatggggc gcaagtaaag gtgggcaaca gaacatatcg tgttgttgtc
      421 actgataata agttttgcgt tacaagagaa agtcatagtg gttgctttac taatctgttg
      481 cacaggctgg gatggcctaa gggagagatt agcaggaaaa ttgaggtgat gctgaattca
      541 tcgccagtga atagggctat ggaaagaggt gctgttcatt cgaatagacc tgatttacct
      601 cctgttgatt atgcaccgcc agagttaccg agtgtggact ataacagctt gcctgtgcct
      661 ggtaatgtta ttggcaaagg gggtaacgct gtagtatatg aagatgctga ggatgcaaca
      721 aaagtcctga agatgtttac tacatctcaa agcaatgaag aggtaacaaa cgaagttcgt
      781 tgcttcaacc aatattatgg tgccgggagt gcagaaaaaa tatatggcga taatggcgat
      841 attattggta ttagaatgga taaaataaat ggagaatcgc ttttaaatat ttcgtccttg
      901 ccggcacagg ctgagcatgc catttatgat atgtttgaca ggcttgagca aaaaggaatt
      961 ctttttatcg atacaacaga gacaaatgtc ttatatgatc gcacgagaaa tgaatttaat
     1021 ccaatagata tatcatctta taatatttct gaacgttcat ggagtgaaaa tcaaataatg
     1081 caatcttatc atggaggaaa gcaagatct
//
LOCUS       DAZGOB010000395         1101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGOB010000395 DAZGOB010000000
VERSION     DAZGOB010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.398"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            123..1082
                     /locus_tag="ACHLIF_005467"
     CDS             123..1082
                     /locus_tag="ACHLIF_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO9609235.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          378 a          209 c          210 g          304 t
ORIGIN      
        1 aaaaacgaat aaatcaattg tctcacgcaa ttataaaaca tcatattgat tacgcacctt
       61 gtattacaaa ctcatgtatg taaaatacgc gcaccattca aaaaaaagga agacaataac
      121 atatgaaaaa aagtgtcatc gctggcgtct ttattgctct gtcatttacc acgtgttcag
      181 ctatcgcgaa cagccttgca ttatcattag caaatgatga tgcagggaag tttcaaccaa
      241 tacttaatga tatttatggc aataaacatg aaaacagaga tgattactca caaggcttat
      301 ttctgggata tagccacgat atctcagact cgagccaatt atctctccat attgcgcaag
      361 atatttactc tccatcaggc agtaataaaa gacacaacac agctgtaact ggagacagag
      421 cttttagtgc atacactcac actggtattg aatggaactc ccttgcgaat gactggattc
      481 gctatcgatt aggtactgac ataggtgttg ttggccccga cgcaggcggt cagaaagtac
      541 aaaataaagc tcatgagatt attggggcag aaaaatatca tgcatgggat gatcaaatag
      601 agaatcgcta cggttatact gtaaaaggga tgctatccat gacaccaagt atggatattt
      661 taggtgctaa tgttggatta taccctgaag tttctgctgt tactggaaac ttatttcaat
      721 atgtagcata tggcgcaacc attgccattg gtaatgataa aaccttcaat tcggataatg
      781 gctttggtct gctggctccc cgtggtttaa tgcatatgtc cgatacaagc ggattcaaat
      841 acaagatttt tgcaggtatg gaaagacgag atgtcaatcg caactatact ctcgaaggaa
      901 aaacaataca gacgaaacaa acaacagtat cgctaaacaa aactgttgat gaatatcaag
      961 ttggcgcaac aattgggtat gcacctgtag ccttcacact agcatttaat aaagtaacat
     1021 cagaattcaa gacaggggat gactattcat ttataaatgg agcaatcacc ttcttttttt
     1081 aactgaattg aattcaatca a
//
LOCUS       DAZGOB010000396         1097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGOB010000396 DAZGOB010000000
VERSION     DAZGOB010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.399"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(22..327)
                     /locus_tag="ACHLIF_005468"
     CDS             complement(22..327)
                     /locus_tag="ACHLIF_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9609236.1"
                     /translation="MATGSKNAKSQSLTARIPHDVIEGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENLLISSLNALAQVEKLGIKAAEEIGQLVTVAREELQRRKTKEPE"
     gene            524..724
                     /locus_tag="ACHLIF_005469"
     CDS             524..724
                     /locus_tag="ACHLIF_005469"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9609237.1"
                     /translation="MRNHTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RLQSPAGCVMVFAARIRQGVGL"
     gene            721..951
                     /locus_tag="ACHLIF_005470"
     CDS             721..951
                     /locus_tag="ACHLIF_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609238.1"
                     /translation="MSQEITLQQAVERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLIDEQERRTLISNEKKCGGVHVQ"
BASE COUNT          302 a          277 c          270 g          248 t
ORIGIN      
        1 acaccacggc gctgatggtg attactctgg ttctttagtc ttgcggcgct ggagttcttc
       61 acgtgcgaca gtgacgagct gcccgatctc ctcggcggct ttgattccga gtttttccac
      121 ctgcgccagt gcattgagcg aagaaatcag gaggttttct ccgcttcctt ctgcctggcg
      181 gcgggcgatt tcaccgcgca tggcggttac tatgaatccg gcgttgcttt caccgtccag
      241 ttttacggat tccatgcctt caataacatc atgcgggatc cgagctgtca gtgattgtga
      301 ttttgcgttt tttgaacctg tagccatctg taatcctctc aatgaaagtg taagacaata
      361 tacacataaa aagtcttaca taaaagcatt gacatgtaag ccacctataa ataaagttac
      421 ttacaccttg ttaatgcaag gtgcagaaac gacgaaaccc cgcactgtag gagcagtaac
      481 ggggcttcta accaccaacg ataacgagag tatcgaggta gctatgagaa atcataccac
      541 acacccgcaa gggcgggact cgcacaacct gaataaatac atttggcgtt ttatcgccct
      601 gagcactgca caaccgcgcg tgattaccat tgaggccacc agcgaacagg aagcacgcct
      661 gcaatccccg gctggctgcg tgatggtatt cgctgcccgt attcgtcagg gggtaggctt
      721 atgagccagg aaatcacact acaacaggca gtagaacgcg cccaccaaat cgaagttatt
      781 tgcgcactgg cagaggatta cccaggcatg atgaccgaca gcgaatcagg ggcaatcatc
      841 ggcttactta aacgccttag cggtgaggtc tgcgtattcc tgatcgatga acaggaaaga
      901 agaacgctta tttctaacga aaaaaaatgc ggaggggtac atgtgcaata aaaccacacc
      961 ggacgcagcc gccgccgcac tcactacgct gatgcacgcg cttattgata ttgaatgcac
     1021 agcagagctt gcacaaaagg aagaaaggga agaaagggaa gaatacacac tattcgccct
     1081 ggaatgtatc cgataca
//
LOCUS       DAZGOB010000397         1081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGOB010000397 DAZGOB010000000
VERSION     DAZGOB010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.400"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1081
                     /locus_tag="ACHLIF_005471"
     CDS             <1..>1081
                     /locus_tag="ACHLIF_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609239.1"
                     /translation="EAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSILVNKTPLTDTD
                     GNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTITSANIDRLRV
                     TFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTTSQYLASVILENL
                     PERPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQ
                     QMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMG
                     KRLGAADVDKWALYAIGQYCDQRVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCSAM
                     RCMPVWNGQTLTFVQDRPSDVVWPYT"
BASE COUNT          246 a          287 c          351 g          197 t
ORIGIN      
        1 gaggcaaagg acaatcttaa gtccacgcag atgatgagcg tgattgatgc gattggtgaa
       61 gggccgattg aaggtccggt gaaggggctg cagagtatcc tggtgaacaa aaccccgctg
      121 acggacacgg acggtaatcc tgtgatacat ggtgtgacag cggtctggcg cgccggggag
      181 caggagcaga caccacctga aggctttgag tcctccgggg cggaaaccgc actgggcgtg
      241 gaagtgacga aagcaaagcc ggtgacgcgc accattacgt ccgcgaacat tgaccgcctg
      301 cgggtcacct tcggggtgca gtcactgttg gagaccacct caaagggcga ccgtaatccc
      361 tcttctgtcc gactgctgat tcagttgcag cgtaacggta actgggtgac ggaaaaggat
      421 gtcaccatta acggcaagac cacctcacag tacctggcgt cggtgattct ggagaatctg
      481 cctgagcggc cctttaacat ccggatggtc cgggagacgg cggacagcac cacggaccag
      541 ctgcagaaca gaacgctgtg gtcgtcatac accgaaatca tcgatgtgaa acagtgctac
      601 ccgaacacgg ccattgtggg gctgcaggtg gatgcggagc agttcggcgg tcagcagatg
      661 acggtgaact accatatccg cggtcgcatc atccaggtgc cgtcaaacta cgacccggaa
      721 aaacgcactt acagcggtat ctgggacggc agtctgaaac cggcatacag caacaacccg
      781 gcctggtgtc tgtgggacat gctgacccac ccgcgctacg ggatggggaa acgcctgggg
      841 gccgcggacg tggacaaatg ggcgctgtat gccatcgggc agtactgtga ccagcgtgtt
      901 ccggacggct tcggagggac agagccgcgg atgacgttta atgcgtacct gtcacagcag
      961 cgtaaggcgt gggacgttct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg
     1021 aacggccaga cgctgacgtt cgttcaggac cgtccgtcgg atgtggtgtg gccgtacacc
     1081 a
//
LOCUS       DAZGOB010000398         1074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGOB010000398 DAZGOB010000000
VERSION     DAZGOB010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.401"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..375
                     /locus_tag="ACHLIF_005472"
     CDS             <1..375
                     /locus_tag="ACHLIF_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO9609240.1"
                     /translation="GATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
     gene            535..672
                     /locus_tag="ACHLIF_005473"
     CDS             535..672
                     /locus_tag="ACHLIF_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609241.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKYILIWLVFVIMASGYFIGQIRW
                     W"
     gene            674..1012
                     /locus_tag="ACHLIF_005474"
                     /pseudo
     CDS             674..1012
                     /locus_tag="ACHLIF_005474"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            984..>1074
                     /locus_tag="ACHLIF_005475"
     CDS             984..>1074
                     /locus_tag="ACHLIF_005475"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO9609242.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGIISSSEN"
BASE COUNT          279 a          244 c          308 g          243 t
ORIGIN      
        1 ggagccaccc gggtggatgg taagcctgtt attcctggca tgaagctgtc gaaggggaaa
       61 tgcgaccagg ttaacgccat tgagcgtgat aaggcgctgg catgggtgga gaaaaacatc
      121 aaagtgccac tgactgaacc ccagaaagcg ggtattgcgt cattctgtcc ttacaacatt
      181 ggcccaggta agtgtttccc gtcgacgttt tataaacgaa ttaatgcagg agatcgaaaa
      241 ggtgcctgcg aagcgattcg ctggtggatt aaggacggtg gcagagactg ccgtattcgc
      301 tcaaataact gttatggtca ggtctcacgg cgtgaccagg agagcgcgct ggcgtgctgg
      361 ggaattgaca gataagcaga atattttgct gaaaaatgcg gtttgctcac acggacggat
      421 aacacaaaat cctgcgaact gacaaaaact aagtgaataa aagtaaaaac cccatttgtt
      481 ggctgcaagc ggggttttgt gtttcctgac tccggaaaag tcaaaggaga aagtgtgttt
      541 gattttagca aactgattcg ggagattcga gtgatggctg aaaaattatc aacctggaag
      601 tacatcctta tctggctggt gtttgtgatt atggcctccg gttatttcat cggtcagata
      661 cgctggtggt gaaatgaacc gcgtactgtg cgtggtcatc attgccctgc tggtggcctg
      721 tggtgcgctt agtctggggc tgaatcatta ccgcgataac gccatcacct acaaagcgca
      781 gcgcgataaa aaagccagag agctggagct ggcaaacgca actattactg atatgcaggt
      841 gcgccagcgc gatgttgctg cgctcgatgc aaaatactcg agggaattag ccgatgcgag
      901 agctgaaaat gaaactctgc gcgctgatgt tgccgctggt cgtaagcgcc tgcggatcaa
      961 cgccacctgc cccggtaccg tgcgtgaagc caccggcacc tccggcgtgg ataatgcaac
     1021 cggcccccga ctggcagaca ccgctgaacg ggattatttc atcctcagag aacg
//
LOCUS       DAZGOB010000399         1073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGOB010000399 DAZGOB010000000
VERSION     DAZGOB010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.402"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..260
                     /locus_tag="ACHLIF_005476"
                     /pseudo
     CDS             1..260
                     /locus_tag="ACHLIF_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            299..>1073
                     /locus_tag="ACHLIF_005477"
     CDS             299..>1073
                     /locus_tag="ACHLIF_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO9609243.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKIFYSNF"
BASE COUNT          376 a          212 c          171 g          314 t
ORIGIN      
        1 ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata
       61 acaaggatgg taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg
      121 atgataagcg tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc
      181 aaatcaatga tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg
      241 atgaagccaa cggctcgcgc ggcaagaatc tctgattgag ttaagtgccc atcacaatat
      301 gatcactgac cttatattac acaatcatcc caggatgaaa acaatcactt taaacgacaa
      361 ccatattgca catttaaacg ccaaaaacac tacaaaactg gaatatttaa acttaagcaa
      421 taacaattta ctgccaacca atgacattga tcaactaata tcatcaaaac atctttggca
      481 tgtattagtt aacgtcatca acaatgatcc acttgcacaa atgcagtact ggactgcagt
      541 aagaaatata attgatgaca ctaatgaagt gaccattgat ttatcaggac ttaatttaac
      601 cactcaacca ccagggctgc aaaacttcac ctctatcaat cttgataata accaactcac
      661 acattttgat gcaaccaact acgatagact cgtaaagcta agtctgaata gtaatgctct
      721 tgagtcaata aattttcctc aaggcagaaa tgtaagtatt acacatatat ctatgaataa
      781 taatgctctc agaaatattg atatagatag gctttcatca gttacttatt ttagtgcggc
      841 acataatcaa ctagagtttg tgcaattaga atcttgcgaa tggctgcaat acctgaatct
      901 cagccataat caattaactg atattgttgc aggtaataaa aatgaactct tactgctgga
      961 tctatcccat aataaactaa caagtttaca caatgactta tttcccaact tgaatacgtt
     1021 acttattaac aacaatttgc tttctgaaat taaaatattc tatagcaact tct
//
LOCUS       DAZGOB010000400         1056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGOB010000400 DAZGOB010000000
VERSION     DAZGOB010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.403"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(26..183)
                     /locus_tag="ACHLIF_005478"
                     /pseudo
     CDS             complement(26..183)
                     /locus_tag="ACHLIF_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            512..>1056
                     /gene="nleG7"
                     /locus_tag="ACHLIF_005479"
     CDS             512..>1056
                     /gene="nleG7"
                     /locus_tag="ACHLIF_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO9609244.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSRE"
BASE COUNT          340 a          236 c          157 g          323 t
ORIGIN      
        1 tgcatacgac gtgccgcgga tcctattatt ctagtatgaa atccctaacc gatttcaaat
       61 tctccagact gctcagattc tcttttatcc cacgctggcg ctgataaatc tcgtcattgc
      121 aatcgacctg cgctatcgct gccgcagttc ttccagtttc tgcatcgata actccatctt
      181 ctgagcaata aacctcacct atgcaatatt ttatgtcaat tatttcaatt gcattaatat
      241 cttgaatgga ttacatagag ttaatgtatc cagtactccc tattctcttg catataatac
      301 atgttgcaac ttacatctca gcgctatgaa aaaacaccac ctctctcttt atgaaattct
      361 ggatttacca agcgctaatt tatcatttca atctaccttt aagcattgca tttatctccc
      421 tacgaggtca tactttagga agttaaatat gaatgataat atacctacag cgcgaaatca
      481 caaacaatcg acttgtatta cagaaaaaaa catgcctatc tttttaaact tcacagcagg
      541 cagtatctta cctgagaatg agctagcatc tttacgttat attgtgcagc aaaatcaaaa
      601 tgatactgta atcataaaag aacgttataa aatggatatc cgttatatcg aatcagtcaa
      661 tggttttaca gtaaatcctg tatgcagtaa tcatttctcc atatttatgg cgagacaaaa
      721 cactattgct cgcaacctgg aacagcagat caacaacgga cgaagttttg cacaaatatc
      781 tcaggatttt atgcttcaat tatcttcaaa tataggatgg aaaaaagggg ccgaaaacgc
      841 ccttaaaaat aaaatccatt ctcattcatt tgttgtaaat cctgatgaat tctcttgcga
      901 cacacaattt cttaagtgcc caataacatt atgcgttcca gaaaaagggg tttttgtcaa
      961 gaacgcactg aactccaaca tatgcactct ttatgataag tctgcgttca tgaatctcac
     1021 aagagaacat ctaccccacc ctctcagcag ggaaaa
//
LOCUS       DAZGOB010000401         1049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGOB010000401 DAZGOB010000000
VERSION     DAZGOB010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.404"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..321
                     /locus_tag="ACHLIF_005480"
     CDS             <1..321
                     /locus_tag="ACHLIF_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9609245.1"
                     /translation="TRRVNVLKGEQRRVSGSARVSLKRRIWLRDAGQCCLCGRVVDLC
                     DSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPDPTLPEVSGGSGRADD
                     IIGL"
     gene            610..>1049
                     /locus_tag="ACHLIF_005481"
     CDS             610..>1049
                     /locus_tag="ACHLIF_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9609246.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTH"
BASE COUNT          245 a          254 c          322 g          228 t
ORIGIN      
        1 acccggcggg tgaatgtcct gaagggtgaa cagcgtcggg tcagtggcag tgcccgggtt
       61 tccctcaagc gtcgtatctg gctgagggat gccgggcagt gctgtctctg tggtcgtgtg
      121 gttgacctct gtgacagtga actcgatcac cgaattgcac ttcagttcgg tggtggtaat
      181 gaggagacga atctctggac gctctgtacc gaatgccatc gacaaaagtc tgctcgtgaa
      241 gcggcgggtg gtatgccgga cccgacgctg ccggaggtgt ccggaggtag tggcagagcg
      301 gacgacatca tcggactgta acccgaccgg gggggtatca ttcggcgtaa aaaacgatcg
      361 ctccggacac cgccccccct ctcacgcaga gaaaaaattc ccgtttcagg gcagttaaca
      421 tgttaactgg ctgtccgggc atttttgcgg tttttatctt tattattcag tttgttgtgc
      481 gaaaaaaatg ttaactggct ttttcagcaa atgttaacca ggcagcagtt aacatttgcg
      541 gcatgagacg ccgggaaaaa tgggctgaac catacccgtc tgagtgcgtt ctggacccgg
      601 gaggaggctg tgctgacaac gcaaaaacga aaatttgcgc tggcgctcat gtccgggaaa
      661 aacaaaacag cgtcagccat tgccgctggt tattcggcga agaccgccag ggttaaaggc
      721 tcgcagctgg caaaagatcc ggaggtgctt gcgtttatag cccgtaaaca atgcgagacg
      781 gtggaggtgg atgaggttcc tgtttaccgg cagaaaaaat cagagcagga ggataaaccc
      841 cgtcgccgtg aggcggctgc aataccacag ccggacgaaa acaatccgga gatgccaccg
      901 tccgcggtga tgtctcctgg tattgaatat atggaggatg gtcttcccga tccggtgaaa
      961 gcgatggggc gtcttctggt ggagaacatt aataccgacc ccaggctggc gctggatgcg
     1021 gcttataagc tggcgcagtt cacgcacca
//
LOCUS       DAZGOB010000402         1042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGOB010000402 DAZGOB010000000
VERSION     DAZGOB010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.405"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..130
                     /gene="kil"
                     /locus_tag="ACHLIF_005482"
     CDS             <1..130
                     /gene="kil"
                     /locus_tag="ACHLIF_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO9609247.1"
                     /translation="HQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINLPWYRRK"
     gene            206..376
                     /locus_tag="ACHLIF_005483"
     CDS             206..376
                     /locus_tag="ACHLIF_005483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609248.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            387..992
                     /locus_tag="ACHLIF_005484"
     CDS             387..992
                     /locus_tag="ACHLIF_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO9609249.1"
                     /translation="MSKDFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
BASE COUNT          352 a          213 c          252 g          225 t
ORIGIN      
        1 tcaccagatg cttcgtgcgg cacaaaacaa agcagtaata gccagattta ttggtgattc
       61 aaaaatgtgg cttgaagcaa ataaagcgat gaaatcagct atcaaccttc cgtggtatcg
      121 caggaaatga gtttcacaga taactggtca gacgaagaat tcattcgtca gatgaaagaa
      181 ttaatcggta acgaaggaga tattcatgtc acttgcaacc acagtgaagg agagcaagtt
      241 acagagacgc atgtacacgc agcaggcgtt aatgtatcgc cagaagggag atcgtgaagg
      301 tgttcgcgta tttttaaatg cggcaaagac tgaagtatta aatcagcgtt atttccttgg
      361 gccatgccca ttctgagaac aaacatatga gcaaagactt ttacgcaaga ctggcagcta
      421 ttcaggagaa cttgaacgcg ccaaagaatc agtacaactc attcggtaaa tataaataca
      481 gaagctgcga agacattctt gaaggcgtta agccgttact gaatggcctg tttttatcaa
      541 tcagcgatga agttgtgttg attggtgatc ggtattatgt gaaagccacg gcaactatta
      601 ccgatggcga aaacagtcat acggcaaccg ctcttgcacg agaggaagaa agcaagaaag
      661 gaatggattc tgcacaagtt acgggagcta caagctctta tgcacgcaag tattgcctca
      721 atggtttgtt cggcattgat gatgcgaaag atgcagatac cgacgagcat aaacatcagc
      781 agaacgcagc agcaaagcag tcaaaaccat cacctacacc tgaacaggtt ctaaaagcat
      841 tcactgacgc agcattgcag aaaaacaccg tggaagagct taaacaggcg ttcgccaaag
      901 cgtggaagat gctcgaaggc acaccggagc agcacaaagc gcaggacgtt tacaacatca
      961 gacgagacga attagaagga gctgctgctt aatggcacat tcgattactg taagactaaa
     1021 caagcccgca agagagtttc ag
//
LOCUS       DAZGOB010000403         1040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGOB010000403 DAZGOB010000000
VERSION     DAZGOB010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.406"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1040
                     /locus_tag="ACHLIF_005485"
     CDS             <1..>1040
                     /locus_tag="ACHLIF_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9609250.1"
                     /translation="TPYTLMQAITAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGL
                     PLPDSYDAPDPRIKQLARRSTVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLS
                     KGQYGCVGQGLHIAKKLLPYIPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSA
                     RWGVGKPLYQDLIARTKAALQKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQ
                     FRADLSVFNAQCHGGSAADVPWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVP
                     FMTDGNGVNTATNAPAEDPDIPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDR
                     LATAILNAAGRTS"
BASE COUNT          242 a          269 c          310 g          219 t
ORIGIN      
        1 accccttata ccctgatgca ggcgattaca gcagaaggtg atgtggtggt cagtggtgca
       61 actgagccgg attggtacta cgtcatcgta ctggccgggc agtccaatgc catggcttac
      121 ggtgaagggc ttccgctgcc ggattcatac gatgctccgg atccgcgcat taaacagctg
      181 gcgcgccgca gtacagttac gccgggtggg gctgcatgca gatataacga tattattccg
      241 gccgaccact gcctgcatga tgtgcaggat atgagtacgc tgaatcatcc gaaggcagac
      301 ctgagcaaag ggcagtacgg ctgtgtcggc cagggcttac atattgccaa aaaactgctc
      361 ccgtatatcc cgaataacgc ggggatcctg ctggtaccat gctgtcgtgg tggttcggca
      421 ttcacccagg gcgcggaggg gacattcagt gcggacacgg gggccagcca ggattcggca
      481 cgctggggtg tgggtaaacc gttatatcag gacctgattg cgcgcactaa agctgcatta
      541 cagaagaacc cgaaaaatgt gttgctggcg gtgtgctgga tgcagggaga gtttgacatg
      601 agcgccgcca cccacgcaca gcaacctgcg ctgtttacag ccatgctgac acagtttcgt
      661 gctgacctct ccgtgtttaa cgcgcagtgc catggtggca gtgctgcaga tgtgccgtgg
      721 atttgtggtg acacgacgta ttactggaaa aatacatacg ctacccagta cgacaccgtg
      781 tacggcgggt ataaaaacag ggagagtgag ggcgtttatt ttgtgccctt catgacagac
      841 ggtaacggtg tcaataccgc cactaacgcg ccggcagaag atccggatat tccggcatca
      901 ggatattacg gtgcggcatc gagaacgaat ggaaaccagg tatcatcaaa ccgcccgaca
      961 catttcagtt catgggcgcg caggagcatt attccggatc gtctggcaac cgctattctg
     1021 aacgcagccg ggcgcacctc
//
LOCUS       DAZGOB010000404         1023 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGOB010000404 DAZGOB010000000
VERSION     DAZGOB010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.407"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1023
                     /locus_tag="ACHLIF_005486"
     CDS             <1..>1023
                     /locus_tag="ACHLIF_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609251.1"
                     /translation="GPIEGPVKGLQSILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQT
                     PPEGFESSGAETALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLLETTSKGDRNPSS
                     VRLLIQLQRNGNWVTEKDVTINGKTTSQFLASVILDNLPPRPFNIRMVRETADSTTDQ
                     LQNKTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYD
                     PEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYC
                     DQTVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSD
                     VVWPYT"
BASE COUNT          229 a          279 c          325 g          190 t
ORIGIN      
        1 aagggccgat tgaaggtccg gtgaaggggc tgcagagtat cctggtgaac aaaaccccgc
       61 tgacggacac ggacggtaat cctgtgatac atggtgtgac agcggtctgg cgcgccgggg
      121 agcaggagca gacaccacct gaaggctttg agtcctccgg agctgaaacc gcactgggcg
      181 tggaagtgac gaaagcaaag ccggtgacgc gcaccattac gtccgcgaac attgaccgcc
      241 tgcgggttac cttcggggtg cagtcactgt tggagaccac ctcaaagggc gaccgtaatc
      301 cctcttctgt ccgactgctg attcagttgc agcgtaacgg taactgggtg acggaaaagg
      361 atgtcaccat taacggcaag accacctcgc agttcctggc gtcggtgatt ctggataatc
      421 tgccgccccg gccctttaac atccggatgg tcagggagac ggcggacagc accacggacc
      481 agctgcagaa taagacgctc tggtcgtcat acaccgaaat catcgatgtg aaacagtgct
      541 acccgaacac ggccattgtg gggctgcagg tggatgcgga gcagtttggc ggtcagcaga
      601 tgacggtgaa ctaccatatc cgcggtcgca tcatccaggt gccgtcaaac tatgacccgg
      661 aaaaacgcac gtacagtggt atctgggacg gcagcctgaa accggcatac agcaacaacc
      721 cggcctggtg cctgtgggac atgctgactc acccgcgcta cggcatggga aaacgcctgg
      781 gggccgcgga tgttgacaag tgggcgctgt atgccatcgg gcagtactgc gaccagacgg
      841 tcccggatgg tttcgggggc acagagccgc ggatgacctt taatgcgtac ctgtcacaac
      901 agcgtaaggc gtgggacgtt ctcagtgatt tctgctcggc gatgcgctgt atgccggtat
      961 ggaacggcca gacgctgacg ttcgttcagg accgtccgtc ggatgtggtg tggccgtaca
     1021 cca
//
LOCUS       DAZGOB010000405         1018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGOB010000405 DAZGOB010000000
VERSION     DAZGOB010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.408"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(113..802)
                     /locus_tag="ACHLIF_005487"
     CDS             complement(113..802)
                     /locus_tag="ACHLIF_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO9609252.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(817..939)
                     /locus_tag="ACHLIF_005488"
     CDS             complement(817..939)
                     /locus_tag="ACHLIF_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO9609253.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          268 a          260 c          194 g          296 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaacc cactcggcag
       61 cgggtttata cattttttac aacataccaa atttgcatga agtatatggc ttttaatcca
      121 gttttgcaat attttgctgt aaaaatgctg ccttttgttt tgaacgtgtt ctcgtcacaa
      181 gcaataaagc atcactatca agctgtagaa aaatgtgctt cattgcaacc cagcgttcag
      241 taaatgtctc ggaccagttt tttgttgtca ctcccaccaa tgatgccagt gtctggtatt
      301 cataggcctc acgccctgca agttcgctct tcacatcctg tgcagccagc cagattaacg
      361 tcttcaggcg atccagtgtc ttacctgcaa tttttctgtt acttaacaaa tctttaaact
      421 cgctccatgc ccattgcgtt atggtgacct gatgccccca tcgaacgctt tcgctgtaac
      481 accaaagcaa ccatgctttc tgatgttcat cgagagacaa aaccgcgcgg cgccatgaag
      541 aggttgagaa ttcaaccggg ctgaccaaag caatggatga accttttgcg tacgactgct
      601 taccggaagt cggcgtatta tccagcgtaa tcatcttgcc agttaccaca tccagaatgc
      661 gcggcttctt tcgtttgtat gtaccagtat caaattgtgc atgctcctgc caggcttcga
      721 gctggccttt cgttgctccg ttcaagtcag cagtagctgc cataagttgc tcacgaacat
      781 actgtaaata ttgggtattc atgcagtaaa tcctttctat attttggcat aattcttcaa
      841 cattcggtaa tcgttcaaaa ccgaatcggg gaaacgacat aagcacagga gcccccagcg
      901 acagcgaagg agttctgata tataagactc agacatcatt cattccccgg ttctccaata
      961 tctgtttcac tcatcatcca taactacctg taattgcccc cccttttttt gtaacagt
//
LOCUS       DAZGOB010000406         1016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGOB010000406 DAZGOB010000000
VERSION     DAZGOB010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.409"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1016
                     /locus_tag="ACHLIF_005489"
     CDS             <1..>1016
                     /locus_tag="ACHLIF_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO9609254.1"
                     /translation="NQSATDTLSSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPF
                     YFLFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTT
                     AQPEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRD
                     SVNQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPD
                     CGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETAD
                     HSIKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKT
                     IDLE"
BASE COUNT          344 a          245 c          256 g          171 t
ORIGIN      
        1 aaatcagagc gcaactgata ctctctcgtc gccagtacca gaaactgatg cagtggaaac
       61 ggcaattccg ggcaacgaaa aaaccgaatg caaagtggaa gtcgaaccat ctgtagagcg
      121 tgaggggccg ttctacttcc tcttcaccga caaggatggc gaaaaatacg gtcgcgcaaa
      181 caaactttct ggtctggaaa aagcactagc cttgggagct acggaaatca caaaagagga
      241 atacttcgca cgtaaaaacg gcacgtactc aggttcacaa caaaatactg gtgcatctga
      301 cacgaccgca caaccagagc cggtaaaagt taccgctgac gaagtaaaca aaattatgca
      361 ggcagccaat atcagccagc ctgacgccga taagttgctt gctgcctctc gcggagaatt
      421 tgttgcaggg attagcgacc cgaatgatcc gaaatgggta aaggggattg aaacccgcga
      481 ttctgtaaac cagaaccagc aagaatcgga acagaacgac cagaaagcgg aacaaaacag
      541 cccaaatacg caacaaaacg agccagaaac gaaacaacct gagccagtag cgcaacagga
      601 accggaaaaa gtctgcaccg cctgcggtca gaccggcggc ggcaactgcc ctgattgtgg
      661 cgcggtgatg ggcgacgcaa cataccagga aacattcgat gaagagtatc aggttgaagt
      721 tcaggaagat gatccggagg aaatggaagg cgctgaacat ccacacaagg agaacactga
      781 cggcaatcag catcacgata gcgataatga aactggcgag acggcagatc actcaattaa
      841 ggtgaacggt catcaagaaa tcacatccac cagcaggacg tgtgaccatc taatgatcga
      901 ccttgaaacc atgggaaaaa atcctgatgc cccgatcatc tcaataggtg caatattttt
      961 cgatccgcaa accggagata tggggccgga atttagtaag actatcgatc tggaaa
//
LOCUS       DAZGOB010000407         1013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGOB010000407 DAZGOB010000000
VERSION     DAZGOB010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.410"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1013
                     /locus_tag="ACHLIF_005490"
     CDS             <1..>1013
                     /locus_tag="ACHLIF_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9609255.1"
                     /translation="IPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGS
                     GEDSRLVTTAITADTEHRSSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAKPAT
                     IELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWSVSGP
                     HIKPGKDFWFYVRSVNLVGKSAFVEASGQASNDAEGYLDFFRGEIGKTHLAQGLWELI
                     DNSQLADEMAEMKTSITETRNEITQTVNKTLEDQSATIQQIQRVQKDTNDDLAALYML
                     KVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPENGNSTPLFVAQGNQL
                     FMN"
BASE COUNT          260 a          256 c          312 g          185 t
ORIGIN      
        1 attccaccgg cagtgcagca cctcacggtg gaggtgagcg cggctgacgg ccagtatctg
       61 gcacaggcga aatgggacac gccgcgggtg gtgaaggggg tgcgcttcag tctgcgactg
      121 accagcggaa gcggagaaga cagccgtctg gtgaccaccg ccatcactgc ggatacagag
      181 catcgttcca gtggtctgcc gctcggggaa tacaccctga cagtcagggc aattaacagt
      241 tatggccagc agggcgaacc ggccaccacc accttccgga ttaacgcgcc agcaaaaccc
      301 gccaccattg aactgacgcc ggggtatttt cagataacgg cggtcccgcg tcttgcggtg
      361 tatgacccga cggtacagtt tgagttctgg ttttcggaga caaaaatcgc agacacatct
      421 caggtggaaa cctctgcccg ttatctgggg accggcagtc agtggagtgt atccggcccg
      481 cacattaagc ccgggaagga tttctggttt tacgtgcgca gcgtcaatct ggtgggcaag
      541 tctgcgtttg tggaagccag cgggcaggcc agcaatgatg cagaagggta tctggacttt
      601 ttcagaggag aaataggaaa aacgcatctg gctcaggggt tgtgggaact gatagataac
      661 agccagcttg cagatgagat ggcggagatg aagaccagca tcacggaaac ccgcaatgaa
      721 atcacacaga cggtcaacaa aacgctggag gaccagagcg ccaccataca gcagatacag
      781 cgcgtgcaga aggacacaaa tgatgacctg gctgcgctgt acatgctgaa ggttcaaaaa
      841 acgaaagacg gcattcccta tgtggccggg attggtgcag ggattgagga tactgatggc
      901 cagccactga gcaacatact gctgctggct gaccgtatcg cgatgataaa tccggagaac
      961 ggcaacagca cgccgttatt tgtggcgcag gggaatcagc tgttcatgaa cga
//
LOCUS       DAZGOB010000408         1008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGOB010000408 DAZGOB010000000
VERSION     DAZGOB010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.411"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            196..>1008
                     /gene="cif"
                     /locus_tag="ACHLIF_005491"
     CDS             196..>1008
                     /gene="cif"
                     /locus_tag="ACHLIF_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFO9609256.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENIQYQKHDNFSFQLFEYDTDNIE"
BASE COUNT          306 a          195 c          230 g          277 t
ORIGIN      
        1 gcatggatgg aggtggtaaa aagtggtgtg gctggcaatc caggccgcgt cacagaaatg
       61 gacaatgccg ctgagagact ggcgaatggc atacaggcac cagaaaaatt tcagacatga
      121 cataatgccc atgccagaag ttctttttgc acctcgcctg tatttataga taaatataga
      181 gtgaatgaat gagatatgaa agacattacc cttccccccc cgacgtccgc gtcctgtctg
      241 acaggggcca tatctgtaaa tactgaagct gtattatctc ccatgcaaca cacttcagcc
      301 ttacatgtaa gagattttgc ttccctgtgc tcacagaacc tcaaagctaa tgtattgcta
      361 aatagtgatg accacgaagt acctatacat cagaaaaatc ctgctgcaat aatgcaaaat
      421 atcgactcta acatcaaaca gatggcaaca gactggggga tgtcgattga ggaggttgag
      481 gttattatag ggcgagagaa aggcattgtg gaaccctcct gcggagttac cgctaatgct
      541 attatgaaac tatttctgga caaggatggc ttcagttact gctttgaaaa tgaacagaca
      601 ctatcgctcg agcagcttca ggagcgcctg tcctgtatgc ctgaatgtaa gagctttgta
      661 ttacgtgtta atgatggtgc gcttggtcat gcttacattg tcgatattcc caaaggagaa
      721 aactcttgtc gtcctgcatt cttgtatcag tcagatttag gagagggcgt caccagaaag
      781 ttaagatttg aggactggat gacgcataaa gcattgactc cgattttgct ggatgatatt
      841 tgtaattact tctcctgcat gtctcaaaat aagacagatt tggagcagat tgcaacgtta
      901 tttgatattg atggaaatgt taaaatgtta cgaaaggaaa atattcaata tcaaaagcat
      961 gacaatttta gtttccagtt gtttgagtat gacaccgata atattgaa
//
LOCUS       DAZGOB010000409         1004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGOB010000409 DAZGOB010000000
VERSION     DAZGOB010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.412"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..867
                     /locus_tag="ACHLIF_005492"
     CDS             <1..867
                     /locus_tag="ACHLIF_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO9609257.1"
                     /translation="AISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRD
                     LLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTL
                     ELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLL
                     DFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTR
                     EATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLR
                     VQSTSKTAPQAK"
BASE COUNT          301 a          227 c          231 g          245 t
ORIGIN      
        1 gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa tttcattgca
       61 ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat gtctgtcatg
      121 gaactgcgcg acctgctgat tgcgctggaa agtcgtatgc atcaaaagca taaactggat
      181 gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa aatggcagaa
      241 aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg tcgggttaac
      301 acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac gctacatgac
      361 ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctagc gcgagcaatc
      421 attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac ctcactgtta
      481 gatttcctgc cgttatatga cgttgactgc caggaaaatg gcaatctgga atatgacacc
      541 tattcccaac cggaatggaa acataacctg ttcgaccatt atctggcggt gctttaccgc
      601 tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac gcgtgaagca
      661 acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag ccaacgactg
      721 aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc gaataacgcc
      781 caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt tcaatccacc
      841 agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc tggagccatc
      901 cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt aaacgcctta
      961 tccggcctac aaattctcaa gcattcaatc agttgcaatg cctg
//
LOCUS       DAZGOB010000410          993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGOB010000410 DAZGOB010000000
VERSION     DAZGOB010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.413"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            19..111
                     /locus_tag="ACHLIF_005493"
                     /pseudo
     CDS             19..111
                     /locus_tag="ACHLIF_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            124..213
                     /locus_tag="ACHLIF_005494"
     CDS             124..213
                     /locus_tag="ACHLIF_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609258.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            233..>993
                     /gene="nleL"
                     /locus_tag="ACHLIF_005495"
     CDS             233..>993
                     /gene="nleL"
                     /locus_tag="ACHLIF_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO9609259.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSIL"
BASE COUNT          295 a          166 c          219 g          313 t
ORIGIN      
        1 ttggcgggat gaagtaaccc gacagcttca caaaaccgga gtccggctcc ggtttttgtt
       61 gtcatgtccg gggaatattt gttagataaa aaagaggaga taattcaata gggagttaaa
      121 ttaatgccga taaatctgac atcttatttg tgggtacagg gacagaaagt tgtcccggca
      181 gttgtgtttt ctaattttac tttagtggac taagtaaaaa ggagtgagat aaatgctgcc
      241 cactacaaat atctctgtaa attctggagt aatatctttt gaaagtcctg tagattcacc
      301 atctaacgag gatgttgaag ttgccctcga aaagtggtgc gctgagggag aatttagcga
      361 aaatcgtcat gaggttgcat caaaaatact tgatgttata agtactaatg gagagacttt
      421 atcaatcagt gagccaataa caacattacc agacttgctt ccaggttctc tgaaagaact
      481 ggttttgaat ggatgtacag agcttaaatc aataaactgc ttacccccca acttatcttc
      541 attaagtatg gttggatgct catcattaga ggttataaat tgcagcatac ctgaaaatgt
      601 cattaattta tctttatgcc attgtagttc tttgaaacat atagaaggtt cctttcctga
      661 ggcactcaga aattccgtat atttaaatgg ctgtaattca ttaaatgaat cgcaatgtca
      721 attccttgca tatgatgtca gtcaaggccg tgcctgcctg agcaaagctg agcttactgc
      781 tgacttaatt tggttgtcgg ctaaccgaac gggtgaagag tctgctgaag aattgaatta
      841 ctctggatgt gacttgtcag gtctaagtct tgtagggctg aatttatcat cagtaaattt
      901 ttctggagca gtgcttgatg atacagatct caggatgagt gatttgtctc aggctgtatt
      961 ggaaaactgt tcttttaaaa actcgatttt gaa
//
LOCUS       DAZGOB010000411          983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGOB010000411 DAZGOB010000000
VERSION     DAZGOB010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.414"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..490)
                     /gene="sctT"
                     /locus_tag="ACHLIF_005496"
     CDS             complement(<1..490)
                     /gene="sctT"
                     /locus_tag="ACHLIF_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO9609260.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFN"
     gene            complement(492..752)
                     /locus_tag="ACHLIF_005497"
     CDS             complement(492..752)
                     /locus_tag="ACHLIF_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO9609261.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(762..>983)
                     /locus_tag="ACHLIF_005498"
     CDS             complement(762..>983)
                     /locus_tag="ACHLIF_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO9609262.1"
                     /translation="EIKSAFIIGFYIYLPFVVVDLVISSVLLTLGMMMMSPVTISTPI
                     KLILFVAMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          348 a          216 c          162 g          257 t
ORIGIN      
        1 aattaaaatt gcctgaaggc cataaattat aactttgcca aagagtttcc agaataaagt
       61 tcattccacc tttagtaaga taaaccgcag cagagaaaag gttaaataat cttgctaatt
      121 ccgaggtatc gacacctgta gccggatcca aagtactact taatgttgca ccgcgttggt
      181 tatcgataaa gctacctata gcaaggaaaa tccaaaacgg ggatgctaat aagcatgcaa
      241 taaacagccc tatgataatt tctctggcag cgaacaaagc aatatcgatt tcatttaagt
      301 tcaataattc atagtggtaa tgctgaacta atgcacatga taccactatt attattgggt
      361 gacgaactac tgttggtata ttcccactgg cgaaaaatgg taacaaatag aaagttggtg
      421 ccaatcggca aaaaccgagc gctgtagcag ccagcagact atgcaactga tagagtatcg
      481 cctcccccat gctatcctct cgcgaatgct aaattcagca tttcgatacc aaatgaatat
      541 aatttttcac cataccaccc gctcattaaa aaaaagcaaa tacttacgca caataatttc
      601 acgccaaatg gcaacgtctg ttcttgcaac tgcgttaccg tttgaaaaag ccctaccaat
      661 aaaccaacaa atgtcgcaac tgcaataggt cctgcagaca taactaaaat cagatacagc
      721 gccctattcc ccgcaaaaac aatatcgtcc atattttttt ctcagggatt aatggatagg
      781 tcaaaatatt gtaaaatgag cccttttgat aacattgtcc aaccgtccat cgcgacaaac
      841 aaaattaatt ttatcggtgt agatatggtt accggactca tcatcatcat acctaacgtt
      901 aacagaacgc tggatataac cagatccact acgacaaagg gtaaatagat ataaaaacct
      961 ataataaatg cactttttat ctc
//
LOCUS       DAZGOB010000412          982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGOB010000412 DAZGOB010000000
VERSION     DAZGOB010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.415"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..366)
                     /locus_tag="ACHLIF_005499"
     CDS             complement(<1..366)
                     /locus_tag="ACHLIF_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9609263.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEK"
     gene            complement(366..>982)
                     /locus_tag="ACHLIF_005500"
     CDS             complement(366..>982)
                     /locus_tag="ACHLIF_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609264.1"
                     /translation="FDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFN
                     PSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          237 a          246 c          226 g          273 t
ORIGIN      
        1 tttttctgct tctgcatctt tcacccaggc tgtgccgttc cacttctgaa actccccttc
       61 cggcgataac caggtaacat tttccggtaa cggaccgagt tcagaaataa ataacgcgtc
      121 ccctgacgct acgtcataaa ccgttttacc ccgatggtct tcaacgagat gccacgatgc
      181 cttatcactg ttgaaaacag ccacaaagcc agcaggaata tctggtggtg caatatcggt
      241 actgtttgct ggcagacctg tatgaggcgg aatatatgcg tcaccttcac caataaattc
      301 attagttccg gccagcagat tataaatttt tatggtccgt ggttgttcac tcattctgaa
      361 tgccattatg caagcctcac aatatagtta aatgcgatgt ttttgacggt gttttccgcg
      421 ttaccagcag cgttaacggt gatggtgtgt ccatgtgaac caatcgcaac ggagtgcgta
      481 tgagcaccaa taccgacagt atgcgcgtgt gcacctgcag atgctgctgt gccggacagt
      541 gaatggctat gattaccatc tgtactggta ttcgctaacc accccgtaga catacctact
      601 gagccttgta caccccaggt attttgacct gagcttgtat aaccatattg ataagtatct
      661 ttaaaaacac tggggttaaa tcgacggcca tctctatggc tgtgattacc agctgcattc
      721 gtgctgccac ttaaactatg ggtatgcgca ccagtgttat tcgtggattt agtgccgtaa
      781 tcaaacgacg atgtggtttt cgtccccaaa tccgtactgg atgcgctggc gctgtgggta
      841 tgcgatttaa tgccgtcctg ttcctgagac aatacggccc gaccactggc aggtttgccc
      901 ttaatcgtcc agccacgcat atcagggatc acgcctgacg gataagccgc tgcaagtttc
      961 gggtatgcag atttgtcaaa ag
//
LOCUS       DAZGOB010000413          981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGOB010000413 DAZGOB010000000
VERSION     DAZGOB010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.416"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>981
                     /locus_tag="ACHLIF_005501"
     CDS             <1..>981
                     /locus_tag="ACHLIF_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9609265.1"
                     /translation="KGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSIL
                     VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTI
                     TSANIDRLRVTFGVQSLVQTTSKGDRNPTSVRLLIQLQRNGNWVTEKDVTINGKTTSQ
                     FLASVILDNLPERPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYL"
BASE COUNT          235 a          264 c          313 g          169 t
ORIGIN      
        1 aaaggtggcg gcagggcgca cacacctcgt gaggcgaagg acaatctcaa atccacgcag
       61 atgatgagcg tgattgacgc cattggtgaa gggccgattg aaggtccggt gaaggggctg
      121 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggtaatcc tgtgatacat
      181 ggtgtgaccg cggtctggcg cgccggggag caggagcaga caccacctga aggctttgag
      241 tcctccgggg cggaaaccgc actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc
      301 accattacat ccgcgaacat tgaccgcctg cgggtcacct tcggggtgca gtcactggtg
      361 cagaccacgt caaagggtga ccgaaacccg acatccgtcc gactgctgat tcagttacag
      421 cgtaacggta actgggtgac ggaaaaggat gtcaccatta acggcaagac cacctcgcag
      481 tttctggcgt cggtgattct ggataatctg cctgagcggc cctttaacat ccggatggtc
      541 cgggagacag cggacagcac ctcggaccag ctgcagaata agacgctctg gtcgtcatac
      601 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cgattgtggg gctgcaggtg
      661 gatgcggagc agttcggcgg ccagcagatg acggtgaact accatatccg tggtcgcatc
      721 attcaggtac cgtcaaacta tgacccggaa aaacgcacgt acagcggtat ctgggacggc
      781 agcctgaaac cggcatacag caacaacccg gcctggtgcc tgtgggacat gctgactcac
      841 ccgcgctacg gcatgggaaa acgtctgggg gcggcagacg tggacaaatg ggcgctgtat
      901 gccattgcgc agtactgcga ccagacggtc ccggatggtt tcgggggcac agagccgcgg
      961 atgaccttta atgcgtacct g
//
LOCUS       DAZGOB010000414          976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGOB010000414 DAZGOB010000000
VERSION     DAZGOB010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.417"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..954
                     /locus_tag="ACHLIF_005502"
                     /pseudo
     CDS             1..954
                     /locus_tag="ACHLIF_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          286 a          227 c          254 g          209 t
ORIGIN      
        1 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
       61 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      121 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      181 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      241 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      301 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      361 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      421 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      481 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      541 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      601 aaagctctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      661 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtttggtctg
      721 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
      781 ggaacgctga aaaatgagag cctgagccac tatcgtttta ataaccggga tgaagccatc
      841 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
      901 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag
      961 aacaaatggt agtgtc
//
LOCUS       DAZGOB010000415          959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGOB010000415 DAZGOB010000000
VERSION     DAZGOB010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.418"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..882
                     /locus_tag="ACHLIF_005503"
     CDS             <1..882
                     /locus_tag="ACHLIF_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9609266.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     RFISPFEYAKINNPLKQ"
BASE COUNT          267 a          229 c          219 g          244 t
ORIGIN      
        1 ttccccttta tcggcaatcg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgccgtaa tgcgttatgc cgtacagtct ggcatcatca gatataatcc tgctctggat
      181 atggctggcg cattgactac ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc
      241 ctgcctgaac ttttgtcgcg tattggcagt tacaaggggc aacctgtcac ccagcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg atccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggttgtag ccatactggc agaacttcag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccacg taaaccaatc agtgaaaaca ccgtaaataa agccctgagg
      601 gttatggggt atgacacaac ccaggatgtc tgtggtcatg gattccgggc gatggcatgt
      661 agtgcgttaa tagaatcagg tttgtggtcc cgcgatgcag ttgaacgtca gatgagccat
      721 caggaacgta acggtgtacg tgctgcttac attcataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt ctggatgcaa acagagaaag atttatcagt
      841 ccatttgaat atgcaaagat taataatcca ttaaaacagt aatcatcccg ggcaaatgcc
      901 cgggaaccta ctccaggatt attttctttg ttaaaaaaac agacaaaacg gtattaact
//
LOCUS       DAZGOB010000416          954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGOB010000416 DAZGOB010000000
VERSION     DAZGOB010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.419"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            29..670
                     /locus_tag="ACHLIF_005504"
     CDS             29..670
                     /locus_tag="ACHLIF_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO9609267.1"
                     /translation="MPVDLTPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEM
                     LENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMN
                     SNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
     gene            701..823
                     /locus_tag="ACHLIF_005505"
                     /pseudo
     CDS             701..823
                     /locus_tag="ACHLIF_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
BASE COUNT          301 a          147 c          222 g          284 t
ORIGIN      
        1 attaagttat taaaatggag ggagaggaat gcctgtagat ttaacgcctt atattttacc
       61 tggggttagt tttttgtctg acattcctca agaaaccttg tctgagatac gtaatcagac
      121 tattcgtgga gaagctcaaa taagactggg tgagttgatg gtgtcaatac gacctatgca
      181 ggtaaatgga tattttatgg gaagtcttaa ccaggatggt ttatcgaatg ataatatcca
      241 gattggcctt caatatatag aacatattga acgtacactt aatcatggta gtttgacaag
      301 ccgtgaagtt acagtactgc gtgaaattga gatgctcgaa aatatggatt tgctttctaa
      361 ctaccagtta gaggagttgt tagataaaat tgaagtatgt gcatttaatg tggagcatgc
      421 acaattgcaa gtgccagaga gcttacgaac atgccctgtt acattatgtg aaccagaaga
      481 tggggtattc atgaggaatt caatgaattc aaatgtttgt atgttgtatg ataaaatggc
      541 attaatacat cttgttaaaa caagggcggc tcatcctttg agcagggaat caatcgcagt
      601 ttcaatgatt gtaggaagag ataattgtgc ttttgaccct gacagaggta acttcgtttt
      661 aaaaaattaa gatagcaatg gcgggttaac ttttcgtaaa atgaaattaa ttctcggtac
      721 ggcatgtaaa acgcggcacc tcgtatgcaa gaacgtactg cggttggttg gtgaactttc
      781 gatagtgcta gtattggatg atttccagcc gttaccgatt ttatataact tcagtggaat
      841 tattcaacac acaggcaaaa cggaataagc tgagaaaaaa agcgtgcttt cccaatctgg
      901 ggtaaatcac gtctgaatga cacctgaaaa caggtggtgg cctcaagaag gctt
//
LOCUS       DAZGOB010000417          951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGOB010000417 DAZGOB010000000
VERSION     DAZGOB010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.420"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..270
                     /locus_tag="ACHLIF_005506"
     CDS             <1..270
                     /locus_tag="ACHLIF_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9609268.1"
                     /translation="VATNNLKLGTFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTP
                     FTLTQSPVSAFAADKLHVTDITRNATYPVLIDSIAVEVNSTDTAA"
     gene            406..585
                     /locus_tag="ACHLIF_005507"
     CDS             406..585
                     /locus_tag="ACHLIF_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9609269.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SAGQSAGSKPEEPLI"
     gene            626..871
                     /locus_tag="ACHLIF_005508"
     CDS             626..871
                     /locus_tag="ACHLIF_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9609270.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
BASE COUNT          248 a          217 c          276 g          210 t
ORIGIN      
        1 gtggcgacaa acaacctgaa actgggaacc tttggcgcat ttgataacga atggcatacg
       61 ctggctttcc gctttgccgg gaataacagc cttcaggtga cgccggttat tgatggtcag
      121 gatggcacac cgttcacgct gacgcagtca ccggtcagtg catttgcggc ggataaactg
      181 catgtgacag acattaccag gaatgcgact tacccggtac tgatagacag cattgcggtg
      241 gaagtgaaca gcacagacac tgcggcatga taaaaaaaac cgccagcgac aggaatggac
      301 gctggcggtg gtgataccta tggagaaaaa ataaaggaac gatactttcg tactctggtt
      361 tttaatgaaa acagttctta ttgtcaacaa taacggaaag aaattatgac atttctgaac
      421 cagttaatgc tgtacttctg tacggtggtc tgtgtgctgt atctcctttc gggtgggtac
      481 agggcagtgc gcgattgctg gcgcaggcag attgacaaaa gggccgctga gaaaatcagc
      541 gccggtcagt cagccggaag caaacccgaa gagccgctca tttagcggca actttcttaa
      601 tcacaccttt cgacgagaaa atcccatgtc agaaattaca tccctggtca ctgctgaagc
      661 agtgaaggaa gtcctgcgct ctgaagaagt ccggagcgca ctgaaacaga aacttcgcca
      721 taacctggaa gcgcgtcttg atgcagaagt ggatgccatt ctggatgaac tgctgggcgc
      781 accggcagct ccggagccgg aaggcatcgc gggtgagggg agtgcttcag atagcggtga
      841 ccccacaccg gacagcgaca tgatgatgta agcatgcgtc agggaccatc ggtgtgtgcc
      901 ggtggtcttt tttattgttg tgagcttccg gattgcggga ggcggggtat g
//
LOCUS       DAZGOB010000418          947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGOB010000418 DAZGOB010000000
VERSION     DAZGOB010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.421"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>947
                     /locus_tag="ACHLIF_005509"
     CDS             <1..>947
                     /locus_tag="ACHLIF_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609271.1"
                     /translation="CTSRGQAHRAGLWVIKTGLLETQTVDFTLGSQGLRHTPGDIIEI
                     CDNDYAGTMTGGRVLSIDAASRTLTLDREVTLPETGAATVNLINGSGKPVSVAITAHP
                     APDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEA
                     IVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRL
                     TSGSGQDSRLVTTAITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPA
                     VPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIA"
BASE COUNT          194 a          283 c          302 g          168 t
ORIGIN      
        1 tgcaccagtc gcggtcaggc ccaccgtgcc gggctgtggg tgataaagac cggactgctg
       61 gaaacgcaga cggtggattt cacgctcggg tcacaggggc tgcgtcacac acccggtgac
      121 atcattgaaa tctgtgataa cgactacgcc ggtaccatga ccggcggacg tgtcctgtcc
      181 atcgatgccg ccagccgcac cctgacactg gaccgtgagg tgaccctgcc ggagacaggt
      241 gccgccacgg tgaacctgat taacggcagc ggtaagccgg tgagcgtggc catcactgca
      301 caccccgcgc cggaccggat acaggtcagc accctgcctg atggtgtgga gacatacggt
      361 gtatgggggc tctccctgcc gtcactgcgt cgtcgcctgt tccgctgtgt ttccatccgg
      421 gaaaacacgg acggtacctt tgccatcacg gcagtgcagc atgtgccgga gaaagaagcc
      481 atcgtggata acggggccag ctttgagccg cagtcaggca ccctgaacag cgtcatccct
      541 ccggcagtgc agcacctgac ggtggaggtg agcgcagctg acggccagta tctggcgcag
      601 gcgaaatggg acacgccgcg ggtggtgaag ggcgtgcgct tcagtctgcg cctgaccagt
      661 ggaagcggtc aggacagccg tctggtgacc accgccatca ccgcggatac agagcaccgt
      721 ttcagcggtc tgccgccggg ggaatacacc ctgacggtca gggcgattaa cagttatggc
      781 cagcaggggg aaccggccac caccacgttc aggattaatg cacctgcggt acccgccacg
      841 attgagctga caccgggcta ttttcagata acagcggtcc cgcgtcttgc ggtgtatgac
      901 ccgacggtac agtttgagtt ctggttttcg gagacaaaaa tcgcaga
//
LOCUS       DAZGOB010000419          937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGOB010000419 DAZGOB010000000
VERSION     DAZGOB010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.422"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(447..937)
                     /locus_tag="ACHLIF_005510"
                     /pseudo
     CDS             complement(447..937)
                     /locus_tag="ACHLIF_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway;
                     frameshifted; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
BASE COUNT          339 a          207 c          117 g          274 t
ORIGIN      
        1 acgatttgcc cagaaataac gaacataatt ttcgttgtaa ttaaaatcat atttcatcta
       61 tagtgaccat tcacagcaaa acaaaatcaa ttatcacatt caaaaaaaat actgacaaca
      121 aagatactct tcagacaaag ttgtcatatg taaattttcc cttaaaacgt tgctcaaaaa
      181 aaatataaaa atcatatgcc ctaatcaaca tcctcaagca ctacgatata tgcagtatcc
      241 tattcatata ttaccaccat atcagacaaa tcgaatcaca acgagttcta tcgtactctt
      301 tcatcttcga cactgtaaat tttaagaaca ttcggctcat gccactaaac caagacataa
      361 taacaacgga gcaataaatg ttcccactga acatgtgcaa acctaaaaag acagtgttac
      421 aataatattc atccatggtc tctaaatcaa cgccacgcaa caggataata caatgaaaat
      481 atttcaggaa aagattcttt tgcatgctcc gttagcatat cagtcagcac agcggtacaa
      541 gtaaaaacat tatttcttcc ttgtagcctg tctaaaatat cattgtaata ataaactgag
      601 ccaaatacct gaggatctgt tttataagct tgttcaatca atccatttgc atatctccgt
      661 agaatctcag gagaatcata ctctgtacca aacatatcac tcgatgagta tttacagaaa
      721 ccccccccct aaacaaaata atatttcagc ctgttttctt agtggcatat ctttcaatac
      781 atggctcccc ataatcatat ctacatgctg agcgttcagt tcccaaccat caagaaaggg
      841 attccagaca caggcaagtt tcctttgctg atcatcagca atcattttat aatctgattt
      901 attggaatta agtgcctcaa taaatctctc tttgagt
//
LOCUS       DAZGOB010000420          935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGOB010000420 DAZGOB010000000
VERSION     DAZGOB010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.423"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            430..899
                     /locus_tag="ACHLIF_005511"
                     /pseudo
     CDS             430..899
                     /locus_tag="ACHLIF_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          267 a          181 c          193 g          294 t
ORIGIN      
        1 acagcgtcat caccttattc agtcgacctt caaaataaac gtttaacaga gacaatcacc
       61 atttggattg tccgctgagg aggcgaacaa tttcctagac agctttatca tgtcagccca
      121 cattgttggc gctggcaata ctaaggacaa acgatgggga agtagctttt tagcgttatt
      181 ttcgggagta aaaaacgtgg tatctttccg tatgtcagtt gtatcttgct ggtattcgta
      241 gtcgtgataa ggcatacaac gatatttatc ttgacgttag tgaagaataa gttgttttgt
      301 gatctatgtt aatctgggat tacaatccgg gcttgataca tcgcatcacg acttttattc
      361 tcttcagaat agaatatgtt actccccgct ttacaggctc aggatttgaa ttacaaatta
      421 tacttttaag tgaacaataa ccaagggtat ttcttatttc ctgaaaccag gcttggccca
      481 tttcgggatg agtttgaaaa aactcttgac gcatacgata gtgatgaaat aaacgaaaca
      541 aattacgtca acaaactcag gcgtcttgcc tgacatgctc ctgacatcat tgatattcac
      601 gctcatctgg catatgcatt tgcgctcttt atgctgcaat tctggcgaat gttcatttac
      661 agcgtcatca ggatgccgtt atactcattg ataaaatact aacatataac cctgagaata
      721 atcagggaac acgctggtta ctgggggcgg aattgttacg tgctggcaag catggcaggg
      781 ctctgtacgt attgacagga catgtcaatg aatttccccc ctactggtac gagcttgctc
      841 ttttacattt tatgaatgag gcatatatta atgctgcaac ggcatttcga tatggatttt
      901 taacaagtat atattgttga aatgctttgt tagaa
//
LOCUS       DAZGOB010000421          935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGOB010000421 DAZGOB010000000
VERSION     DAZGOB010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.424"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..224
                     /locus_tag="ACHLIF_005512"
     CDS             <1..224
                     /locus_tag="ACHLIF_005512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609272.1"
                     /translation="QRYDDSGVICTGGPGTLSGIATTKNSKSKGNGAGGVGGNRLIFR
                     YYRPVSAEGGSYLSGLRIPLDSITDWRNT"
     gene            complement(337..533)
                     /locus_tag="ACHLIF_005513"
                     /pseudo
     CDS             complement(337..533)
                     /locus_tag="ACHLIF_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            709..>935
                     /locus_tag="ACHLIF_005514"
     CDS             709..>935
                     /locus_tag="ACHLIF_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9609273.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          247 a          249 c          234 g          205 t
ORIGIN      
        1 agcagcgcta cgatgactcc ggtgttatct gcaccggagg accgggaaca ttaagcggta
       61 tagcgacaac aaagaattcg aaatcaaaag ggaatggtgc cggaggcgtg ggcggtaata
      121 ggctgatttt caggtattac cggcctgtat ctgctgaagg gggctcgtat ttgtcggggc
      181 tgcgtattcc gctcgattcg atcacggatt ggcggaatac ctaactgtga ttgattcagg
      241 taccattttg cagggatccg gtgcgttact gtctgacaca acaaacgcag cgggtcgctc
      301 tgctccgccg attcctgata atccctcccc agtaacctac tgcaacagta caaatacact
      361 gacgcctgaa acggtaacca gaatcagcac cagcagtaac tcagtaaaca gagcagacga
      421 tcggtcttcc ggtaataacc ggaaggaacg acagcgccac aatacaggca acacactgta
      481 ttatgagtaa tcgaattacc gcaatccccg catttctttt tgcgtatcac gatagctccc
      541 tcgccacgcc ttatccgtaa ccggtttttt acattaaaaa aaacaacgcc gggaattcaa
      601 tcgtcagtcc ggagacgacc gttcgggtta tcacagagag cctgagcagg ggggctgcgc
      661 attttttacg atgtggctat tccgtatgaa ccatacggag atataaccat gacctacaaa
      721 tacaacccct tctggcagca acgtattcgt gagacggtgc ggcacgcact gaatgttcat
      781 ccccgcctga cggcattgcg ggttgacctg cgtttcccgg atgtaccggc agcaacggac
      841 gcagctgtga tatcccgctt catcaatgcc ctgaaagccc gaatcgacgc ttaccagaaa
      901 cgtaagcatc gggaaggtaa acgcgtgcat cccac
//
LOCUS       DAZGOB010000422          932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGOB010000422 DAZGOB010000000
VERSION     DAZGOB010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.425"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..688)
                     /locus_tag="ACHLIF_005515"
     CDS             complement(<1..688)
                     /locus_tag="ACHLIF_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9609274.1"
                     /translation="MTETESAILAHARRCAPAESCGFVISTPEGDRYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDACHLAGITLPDFHREDEWWRNGQNLYLDNMEAT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWH"
     gene            complement(693..>932)
                     /locus_tag="ACHLIF_005516"
     CDS             complement(693..>932)
                     /locus_tag="ACHLIF_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9609275.1"
                     /translation="TPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPT
                     TDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
BASE COUNT          199 a          300 c          245 g          188 t
ORIGIN      
        1 cgtgccagtg gcggtgacgc cagacagaat gcgttcgtcg ttgccatttt tcggaatacc
       61 tctcccgttt actcagttgt tcaggcagat ggtgaagcag ttcaccgttg ccgcagtaaa
      121 tggcagcatg gttgggtacc gatgcgccaa agcagcacag caggatatcg cctgcctgtg
      181 ccgaggacag gggcacccgg taaaaaccag tggcctccat attgtccaga tacaggttct
      241 gaccattgcg ccaccactca tcctcgcggt gaaaatccgg cagcgttatc cccgccagat
      301 ggcaggcatc cctgaacagc gtgtaacagt ccgtcacccc gtgttcaaaa cgacgcccgg
      361 tcaggtgcgg cacacagcgg aatttatgaa tgtccccccg gcagaccagc caccagggca
      421 gggcactttt tatctgcagc cgccggtcgg cctcgctcag ccagggcagg ccaccgggat
      481 ggctgtggac cagtgccaca atctccccct gcatctctgc ccgcagccag tcttccgggg
      541 caatacgaaa atatgcctca ggctctgcag agatattcac gcacggctgg taccggtcac
      601 cctccggcgt gcttatcacg aagccgcaag actccgcagg cgcacaccgc cgggcatgcg
      661 ccagaatcgc tgattctgtc tctgtcatgg atttactgcg aaagtttatt aatggaaagg
      721 aaaccgccaa aattagccac catgccgcgc atctcacacc cgcgcatgca cttgctgcat
      781 ctgtccttac ggatatccgt ggtggggttg tcgaactcat ccgccaccgc aggaccgtta
      841 tacccgcatt catctccccg gtaatcccac atacaggtgt tcgccagcat gatgcgacca
      901 ggaaacagcg ccccgtccgt ctccgtcggt gt
//
LOCUS       DAZGOB010000423          931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGOB010000423 DAZGOB010000000
VERSION     DAZGOB010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.426"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..117
                     /gene="gpW"
                     /locus_tag="ACHLIF_005517"
     CDS             <1..117
                     /gene="gpW"
                     /locus_tag="ACHLIF_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO9609276.1"
                     /translation="RRIEYTAASLDELNRAINDAESVLGTTRRRRRPLGVRL"
     gene            114..>931
                     /locus_tag="ACHLIF_005518"
     CDS             114..>931
                     /locus_tag="ACHLIF_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9609277.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          189 a          224 c          321 g          197 t
ORIGIN      
        1 cgcagaattg aatatacggc ggcttctctg gatgagctta accgggcgat caatgatgcg
       61 gagtcggtac tggggacaac ccggcgtcgc cgtcgtccgc tgggagtgag gttatgaaac
      121 gaacgcctgt cctgattgat gtgaacggcg ttccgcttcg tgagagtctc agctacaacg
      181 ggggcggtgc aggatttggc gggcaaatgg ctgagtggtt gccaccggcg cagagtgccg
      241 atgcggccct gctgcccgcg ttgcgtctgg ggaatgcccg ggcagatgat ctggtgcgca
      301 ataacggaat agcggctaat gcggtggctc tgcataagga tcacattgtc gggcatatgt
      361 ttctgatcag ctaccgtccg aactggcgct ggctggggat gcgggagacc gcagcaaaaa
      421 gctttgtcga tgaggtggag gcggcctggt cggaatacgc cgaagggatg tctggcgaga
      481 tcgacgtgga aggaaaacgc acgttcacgg aatttatccg tgaaggtgtg ggcgttcatg
      541 cgtttaacgg cgaaatcttt gtgcagccgg tctgggatac ggaaaccacg cagttattcc
      601 gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatgggga
      661 accgttttct gcgggccggg gtggaggtcg atcgatatgg ccgtgccgtt gcgtaccata
      721 tctgtgagga tgattttccg ttctccggga gtggacgatg ggaacggatc ccgcgtgaac
      781 ttcccaccgg gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc
      841 gtggggccaa ccagttttac agcgtcatgg aacggctgaa gatgctcgat tccctgcagg
      901 caacacagct tcagtcggcc atagtgaagg c
//
LOCUS       DAZGOB010000424          917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGOB010000424 DAZGOB010000000
VERSION     DAZGOB010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.427"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>917
                     /locus_tag="ACHLIF_005519"
     CDS             <1..>917
                     /locus_tag="ACHLIF_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFO9609278.1"
                     /translation="IAIQSIISKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALTNIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICP
                     YAFMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTHADAFRESLPIIS
                     GELYFEAHQGCFTSESATKAHNRIMENKL"
BASE COUNT          289 a          193 c          181 g          254 t
ORIGIN      
        1 aaattgcaat ccaatccata attagtaaaa acaataatcc ttcagagttc aggattatgg
       61 ctactggtca tgcccattta gatatagcgt ggatgtggcc tttaagagaa ggacgcagaa
      121 aagcaataag aacatttgcc accgccctga caaacattga aaaatatcct gattacattt
      181 ttggcgctag tcagtaccaa ctttttcact ggataaaaaa agattatccc tatttcttcg
      241 aaaagttaaa aaaacaaatc gccgcaggcc gctttgagtt acaaggttgc ttttgggtcg
      301 aatgcgattt aaatctggta agtggtgaat cgctgataag gcaaatcatg catgggacac
      361 gattcacact gcagaatttt tcaaagaaga taaattatgt ctggcaaccg gatgtctttg
      421 gattccccgc cacgcttcca caaattctca aaaaatcagg cattaactat attgcatcgc
      481 aaaagctctc acaaaacaag attaataaat ttaataatta ccttttccgc tggcaaggcc
      541 tggatggctc agaaattctg atgcataact ttcctgaaga tacgtatgat agccgagccc
      601 gcgctcgctc tttagaatat attgaacaaa actataatga aaaagaaatc tgtccctatg
      661 cgtttatggt gtatggtgtc ggtgatggcg gcgctggccc cggtgaggaa catatagaac
      721 gattaacacg catacgcaat attgatggct tacctcatgt tgatttcagc cgtgttgata
      781 agttcttcac tcacgccgac gcctttcgcg aatccttgcc cattatttcg ggtgaacttt
      841 acttcgaggc tcatcaaggg tgctttacat cagagtcagc aactaaagcc cataatcgaa
      901 ttatggaaaa taaactc
//
LOCUS       DAZGOB010000425          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGOB010000425 DAZGOB010000000
VERSION     DAZGOB010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.428"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>916
                     /locus_tag="ACHLIF_005520"
     CDS             <1..>916
                     /locus_tag="ACHLIF_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO9609279.1"
                     /translation="TVNLIKSARVGYTKMLLGVVGYFIEHKSRNSLLFQPTDSAAEDF
                     MKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLGGAAAKNYREKSV
                     DVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIKGTCQIEKAANES
                     AHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQ
                     SNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDA
                     LKDPNGLKTFVNTTLGETWEEAVGEKLD"
BASE COUNT          210 a          230 c          260 g          216 t
ORIGIN      
        1 gcactgttaa tctgattaaa tctgcccgtg ttggctatac aaagatgttg ctgggggtgg
       61 tcgggtattt tattgagcat aaatcccgaa acagtctgct ttttcagccc acggattctg
      121 ccgctgaaga ttttatgaag tctcacgtgg aggcgacgat tcggaacgtg ccatgcctga
      181 aagacctttc cccatggctg ggtcgtaaac atcgtgacaa tactctcacg ctgaaacgct
      241 tttcatcggg cgtcggtttc tggtgcctgg gcggcgctgc cgccaaaaac taccgtgaaa
      301 aatccgtgga cgtggtctgc tatgacgaac tttcctcgtt cgagccggat gtcgaaaaag
      361 agggctcgcc aaccctgctg ggggataagc gtattgaggg gtcggtgtgg ccaaaatcca
      421 ttcgcggctc gacgcctaaa atcaaaggca cctgccagat cgaaaaagcc gctaacgagt
      481 cggcgcattt tatgcgtttt tatgtgccct gcccgcactg tggggaggag cagtatctga
      541 aatttggcga tgagtccacg ccttttgggc ttaaatggga gaaggacagc cctgaaagtg
      601 ttttctacct ctgtgaacat catggctgcg tgatccatca gtctgaactg gaccagagca
      661 acgggcggtg gatctgtgaa aacacgggca tgtggacccg tgacggtctg acgtttttca
      721 gcgcccgggg tgatgaaatt ccgccgccgc gctccatcac gttccatatc tggacggcgt
      781 acagtccgtt caccacctgg gtacagattg tctatgactg gctggatgca ctgaaagatc
      841 ccaacggcct gaaaaccttt gtgaacacca cgctgggcga gacctgggaa gaggccgtgg
      901 gcgaaaaact cgatca
//
LOCUS       DAZGOB010000426          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGOB010000426 DAZGOB010000000
VERSION     DAZGOB010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.429"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..495
                     /locus_tag="ACHLIF_005521"
     CDS             61..495
                     /locus_tag="ACHLIF_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO9609280.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          222 a          204 c          274 g          216 t
ORIGIN      
        1 gtactgcaca cactgccaga ccggaggatt cagtattccc cggacaactc aggaggaaat
       61 atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa
      121 gaagctgcga agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta
      181 acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc
      241 gctgaaaaaa tcctgctgcg atgtacctgg cttgacccgg cggagaaaga ggcccgtcgt
      301 ttgcatctgc agggcatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt
      361 gtgatgtgtg acgaagatta tgagggggaa aagtgctgcc cacgctgcgg taccggggta
      421 tacagcaggg cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg
      481 ttacagaggc tgtaatggtt gcctccggag cagtttgcgg tgatgccttt cccgggaatg
      541 gattgccagt gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccgttgattt
      601 tatgccaaca gccccctgtg actgacaggc tgcctcgtca ttccattcgt atctccagta
      661 acagggggtt gtattgtatt ttattgtgcc ggttcaggtg tgcgtttccc ggcgcgtctg
      721 caccggctta accaaattca acagggataa aatagtggta agcgtacagc ctgaaccgtc
      781 tggtcagaat ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaaccaaa
      841 gtgtcagata tgcagaaaaa tgtgactccc ggcaggcgaa aaggctgccc taattatcct
      901 cccgaattta aacagc
//
LOCUS       DAZGOB010000427          915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGOB010000427 DAZGOB010000000
VERSION     DAZGOB010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.430"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(173..391)
                     /gene="ydfC"
                     /locus_tag="ACHLIF_005522"
     CDS             complement(173..391)
                     /gene="ydfC"
                     /locus_tag="ACHLIF_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO9609281.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(463..762)
                     /locus_tag="ACHLIF_005523"
     CDS             complement(463..762)
                     /locus_tag="ACHLIF_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609282.1"
                     /translation="MALYIDISAIAGQVRIIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IKLRGLIFSYKVTTIFADGSRETVRAMRFKGCVKDFATTFWARKLDCIHNQFPLE"
BASE COUNT          195 a          240 c          208 g          272 t
ORIGIN      
        1 atctatgaac cagctacctc gccgtgcgct ttcgcgttat ggtctgactt ttcagggaaa
       61 tatcctttca gtaaactgtc agtgccggat gctcacccgt gtccggcgca cgcactccac
      121 ctcacccgtg gagaactcct taattaccaa ccttagcttc gttggttagc tattaacgcg
      181 ggtatgtaat cattctggca atgcttaatg ccgctgcttt ttccagattg gtgatatcct
      241 gctccagagc ggacagattt tcagcctgct tagccctggc ttcattggcc catttcaggt
      301 cctgcgctgc cttaattttc tggtgcatcc actcataaag ttcatcatcg gtatagtctg
      361 gcgcgatgat gacgggttct cgtttctgca tgtcggctcc ttgtggttag cgctgcctgc
      421 ttttaaccac gtcaggcgag gtggtatcct ctgaggggtc tgttactcga gaggaaattg
      481 gttatgaata caatcaagtt ttcttgccca gaatgtggtg gcgaagtctt tgacacatcc
      541 tttaaaccgc atggctctga cagtttcgcg ggagccatct gcaaaaattg tggtcacctt
      601 gtaactgaag atgagtcctc gcagtttgat gacgaaatcg ttgacaatat cttcggtgca
      661 ctcaccagag actttctgaa gtaaaggcgc ataccgctta gttaccgctc tgataattct
      721 tacctgtccg gcaatggcgc tgatatcaat ataaagcgcc atcgctgttt ctttgctgat
      781 ccctggatgc cttccattct gatgtttgac ttcgcccact gagaaatcct ctgtttcccc
      841 ttaacgccgg ggtagcggaa caaaaaacct gctgcatagt taaacgtaat ccccgccgtc
      901 atgttcatac gcctc
//
LOCUS       DAZGOB010000428          912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGOB010000428 DAZGOB010000000
VERSION     DAZGOB010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.431"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            160..756
                     /gene="fimE"
                     /locus_tag="ACHLIF_005524"
     CDS             160..756
                     /gene="fimE"
                     /locus_tag="ACHLIF_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO9609283.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          265 a          165 c          201 g          281 t
ORIGIN      
        1 atcagtgata ttttattttt gtatgatatt taatgtaatt gactgatagc cacatcactt
       61 cgtgtgtggt tatcttttta tctattgggc taattttgac cgattgaggt ttcctatagg
      121 tattcattca aatatatctc agtcaggagt actactattg tgagtaaacg tcgttatctt
      181 accggtaaag aagttcaggc catgatgcag gcggtttgtt acggggcaac gggagccaga
      241 gattattgtc ttattctgtt ggcatatcgg catgggatgc gtattagtga actgcttgat
      301 ctgcattatc aggatcttga ccttaatgaa ggtcgaataa atattcgccg actgaagaac
      361 ggattttcta ccgttcaccc gttacgtttt gatgagcgtg aagccgtgga acgctggacc
      421 caggaacgtg ctaactggaa aggcgctgac cggaccgacg ctatatttat ttctcgccgt
      481 gggagtcggc tttctcgcca gcaggcctat cgcattattc gcgatgccgg tattgaagct
      541 ggaaccgtaa cgcagactca tcctcatatg ttaaggcatg cttgcggtta tgaactggcg
      601 gagcgtggtg cagatactcg tttaattcag gattatctcg ggcatcgaaa tattcgccat
      661 actgtacgtt ataccgccag taatgctgct cgttttgccg gattatggga aagaaataat
      721 ctcataaacg aaaaattaaa aagagaagag gtttgattta actcattgat aataaagtta
      781 aaaaacaaat aaatacaaga caattggggc caaactgtcc atatcataaa taagttacgt
      841 attttttctc aagcataaaa atattaaaaa acgacaaaaa gcatctaact gtttgatatg
      901 taaattattt ct
//
LOCUS       DAZGOB010000429          911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGOB010000429 DAZGOB010000000
VERSION     DAZGOB010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.432"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..283
                     /locus_tag="ACHLIF_005525"
                     /pseudo
     CDS             1..283
                     /locus_tag="ACHLIF_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(302..911)
                     /locus_tag="ACHLIF_005526"
                     /pseudo
     CDS             complement(302..911)
                     /locus_tag="ACHLIF_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT          294 a          180 c          147 g          290 t
ORIGIN      
        1 ggttaatgca gataaaccag atgcatattc ccctgttaaa aaaacgtgga ataataaaag
       61 atgaacgaga tttgttagat aacccttgtc tgaacattaa aataggaacc gagattttat
      121 ataaccattt ttcacgttgt ggagtaacgt ggcaatgcct ggggacttat aatgcgggtt
      181 ttgcaatgga taaccaaaaa aagagacagc aatatgcccc aaaatataca ttgtatatac
      241 caggcttaat gaactagaca atcgtaaggc ttcagcaaaa tgagcctaaa gcctcttttt
      301 tttatatagt gcaaacaccc atacgtaaag ctgttttgat caactcgata ccactgtgaa
      361 catctaattt cttcatgata ttgagtctat gagtttcgac tgtttttctg cttaactgca
      421 agaaattagc gatttctttg tttgttttac catcagctat gagttgtaat atttctgact
      481 cacgatttgt aagttgatta tcagaggaat acctggaaga tattttttca caatccatat
      541 gtacactatc aaaataagtg taaccattac taatcgaatc aatagcacga tttagtttcg
      601 ccgggtgact ggttttccat acgcatcctt tcgcacctgc ttcaagcaat tttattgaat
      661 caatatacgg catctttgct acaaatgtaa taatatgagc atctatctta tgcgatttaa
      721 cccattttac gagttcggac cccatcattc cacctgcaaa ttctcctaaa aaaaatattt
      781 aggctgtaga tttgttacac tccgctatag ctttcttcag atctttatag cccccaacaa
      841 cctcatataa atcaggataa tgtccgagaa atccaattat cccatcaatc ataaacggct
      901 gctgatctga a
//
LOCUS       DAZGOB010000430          907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGOB010000430 DAZGOB010000000
VERSION     DAZGOB010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.433"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(106..626)
                     /locus_tag="ACHLIF_005527"
                     /pseudo
     CDS             complement(106..626)
                     /locus_tag="ACHLIF_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            670..750
                     /locus_tag="ACHLIF_005528"
                     /pseudo
     CDS             670..750
                     /locus_tag="ACHLIF_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          262 a          171 c          196 g          278 t
ORIGIN      
        1 aactgcaatt atttgtaaac ataataataa gtattatgac gttaaaattt aaccatacgg
       61 taaaatgatt caggaaaacc ggttcagcat agcctaggct gaacttttga ggatatcaac
      121 gaacggaatt tttgaacatt tatgagtaga tcgttgttga agggcttcaa tgagcatgtc
      181 aagttcatca actgctggtc tacgattacg acggtttgat ttaccaatca aagcaagttt
      241 aagtagatat ggacgagcac ttttcgccgg gtttgatgtg taattaattc cgtatatagg
      301 tttggctgca tccagaacac tgccaagata actaacatcg tgactgactg ttgctggacc
      361 tgcaccagcg ttgtttctca gcctgcaatg ttcaattacg tcattttctg tcagttcaga
      421 tagtttgatc gcggagatgt cactatccat aagcagttct agcacatatc ttttagtacg
      481 gtctgcttta cctccggcat ttggtcattt aaatatttgt gtagtaagtc acggactgta
      541 agtccgtcaa cagcatttga tgatgaaatg ccatatagat ctaataccat cactttctgt
      601 gtgccccatg ttttagcatg agcatgaacg cggcggctat ctcacgggtg atcatccctt
      661 tggcatactg aaaagtatcg ggtgttactg aaccgtgttg atacatcaac tgcactggat
      721 attgagtggc cgataatacc gacgttataa cctataaatg ccaatcaggc tgaatgttga
      781 aaggatagaa tgaacagccc gacacacaaa aaacggagtc cggctccggt ttttgtgttg
      841 ccatataagg cagatgtttg ttacagctat ttaagtctgg agttcaaatt aaaataggga
      901 gttttgt
//
LOCUS       DAZGOB010000431          903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGOB010000431 DAZGOB010000000
VERSION     DAZGOB010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.434"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..607
                     /locus_tag="ACHLIF_005529"
     CDS             <1..607
                     /locus_tag="ACHLIF_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9609284.1"
                     /translation="ISLLPAAGEAAAQGWSIKDGGIQLSDGVFKITKQSNKTWSLTHP
                     VDDAITLLTQGGRLTCKFRLSGALTNNQFGLGIYLYTDAPVPDGVAMTGTGNPFLMSY
                     FTQTTDGRVNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGSATVTPKLNGVAGPAF
                     QVIKDSLTLGLNALTLTDVTKNAAYGVEIESLVLEINAPAA"
BASE COUNT          241 a          194 c          254 g          214 t
ORIGIN      
        1 aatctccctg ctgccggcag ccggagaggc tgctgcgcag ggctggagca ttaaggatgg
       61 cggaattcag ttgtcagatg gtgtatttaa gatcaccaag cagagcaata aaacctggtc
      121 cctgacgcat ccggtggatg acgcaattac cctgctgaca cagggcggca gactgacctg
      181 taagttccgc ctgtcaggcg cactgaccaa caatcagttc gggctgggga tttatctgta
      241 tacggatgct cccgttcctg atggtgtggc gatgacgggt accggtaatc cgttcctgat
      301 gtcgtacttc actcagacca ctgacggcag agtgaatctg atgcatcaca ggaaagccgg
      361 aaacacgaag ctgggggagt tcggcgatta cggtaacgac tggcagacgc tggagctggt
      421 gttcaccgcc ggcagtgcca cggttactcc gaaactgaat ggagtggctg gcccggcatt
      481 ccaggttata aaagacagtc tgacactggg actgaatgcg ctgacgctga cggatgttac
      541 aaaaaatgca gcgtatggcg ttgagataga aagtctggtg ctggagataa atgcaccggc
      601 agcataataa aaaaagagcc agcgactgac ctgaaagaag acgctggcta aaaggcctta
      661 tatgtttgta gagacttatt tttcacagac agcaatgatg cctgtcaata tattatcaat
      721 atgcggattg tttcagttac agatgcttta ttaaggaaaa aaacagccag cactgacttt
      781 cggtggagag gtgctggctc agaaggatag ttggatttca catgatactt atgcctggcg
      841 gtatattttc tgacagacag tgacgggtgt tgtcaagata ttgtgtcatt tataacctga
      901 atc
//
LOCUS       DAZGOB010000432          895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGOB010000432 DAZGOB010000000
VERSION     DAZGOB010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.435"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..153)
                     /locus_tag="ACHLIF_005530"
     CDS             complement(<1..153)
                     /locus_tag="ACHLIF_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9609285.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDA"
     gene            complement(143..>895)
                     /locus_tag="ACHLIF_005531"
     CDS             complement(143..>895)
                     /locus_tag="ACHLIF_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9609286.1"
                     /translation="SELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNNVTLGG
                     VKIPHLFPGDDLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSA
                     RASANESWRYFMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSR
                     AEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQK
                     AGLSRPVWIAQAYEQQIAESRRPEEETTPRET"
BASE COUNT          207 a          270 c          217 g          201 t
ORIGIN      
        1 agcatcaaac tgtacctgct gttgtggtac cgaaagactt gatgccccca tctcgcgccc
       61 gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggtt cattgaatgc
      121 tgcggcaata atgtgtgaaa gattacgtct cacgtggtgt tgtctcctct tccggcctgc
      181 gactctccgc tatctgctgc tcatacgcct gcgctatcca caccggacgt gagagtccgg
      241 ctttttgtcg ctcagcagat tccctgacct gctggcggaa aatgtcctga taatcctcgc
      301 ccatcagcgc cagctctttc tcatacgtgc tcagtccggc ctcaatgcgc atcactgatt
      361 cctggacttc cttgagcccg tcaatggcca ttcttccggc tccaatccac tcagcccgtg
      421 accaggctga tcgcgcctga taaaaatcaa aacgcgcccg tggcggacga ataatccccc
      481 gaagaagtgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg gacgcaataa
      541 attttcgtcg tcccataaaa tagcgccacg actcattggc ggaggcgcgg gcacttgaat
      601 aactgacctt cgagtaatcc cgggacaact gttcgtagga aacgccaaga ccggcggcga
      661 tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc tgcgcagtct
      721 gcagtttcag atcatcaccg gggaaaaggt gcggaatttt gacaccgccc agcgtcacgt
      781 tattcgtgtc ataccaggtg gagaacttat ccagaatatt aataagcgga ttatccttct
      841 gcccctgcgg cgcaccggcg atatattcaa aggccttttc ggtatcaagt tcact
//
LOCUS       DAZGOB010000433          887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGOB010000433 DAZGOB010000000
VERSION     DAZGOB010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.436"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..243
                     /gene="gpU"
                     /locus_tag="ACHLIF_005532"
     CDS             <1..243
                     /gene="gpU"
                     /locus_tag="ACHLIF_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO9609287.1"
                     /translation="ALDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPAMSAIPAL
                     SGLITTMVTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            251..>887
                     /locus_tag="ACHLIF_005533"
     CDS             251..>887
                     /locus_tag="ACHLIF_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO9609288.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGQGDAYANPLSDDNWQRLAQVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     VTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDM"
BASE COUNT          224 a          200 c          279 g          184 t
ORIGIN      
        1 gcgctggaca gcgatacctg gcaggcggag ctgcatattg aagttttcct gcctgctcag
       61 gtgccggatt cagagctgga ttcgtggatg gaaagccgga tttatccggc gatgagtgcg
      121 atcccggcac tgtcaggcct gattaccacg atggttacgc agggctatga gtatcgtcgt
      181 gatgacgata tggcgttatg gagttctgct gatttgactt attccattac atacgagatg
      241 tgaggacgat atgccaacac caaatcctct ggcaccggta aaaggtgccg gtaccaccct
      301 gtgggtttat aacggtcagg gtgatgccta tgcaaacccg ttgtcagacg ataactggca
      361 gcgactggca caggtaaagg atctgacgcc gggcgagatg acggcagaac cctacgatga
      421 taactacctg gatgatgaag acgcggactg gactgcgacc gggcaggggc agaagtctgc
      481 aggagatacc agttttacgc tggcctggaa accgggagaa gaaggtcaga aagggcttat
      541 aggctggttt gaaagcgggg atgtgcgggc ctataaaatc cgtttcccga acggcacggt
      601 ggatgtgttc cgtggctggg tcagcagtat cggtaaggcc gtaacggcga aggaagtgat
      661 cacccgtacg gtgaaagtga ccaacgtggg taaaccttct gtggcggaag aacgcagcga
      721 aattacgccg gtcactgcga ttaaggtgac gccgacatct ggtacggtgg caaaagggaa
      781 aacaacaacc ctgacggttt cttttgagcc ggaaagtgca accgacaaaa cgttcagagc
      841 ggtttccgcc gatccgtcaa cgggaaccat tgctgtgaaa gatatgg
//
LOCUS       DAZGOB010000434          886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGOB010000434 DAZGOB010000000
VERSION     DAZGOB010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.437"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>886
                     /locus_tag="ACHLIF_005534"
     rRNA            <1..>886
                     /locus_tag="ACHLIF_005534"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          209 a          202 c          279 g          196 t
ORIGIN      
        1 cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt
       61 cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc cacccgagac
      121 tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg gaaagacccc
      181 gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg ataggtggga
      241 ggctttgaag tgtggacgcc agtctgcatg gagccgacct tgaaatacca ccctttaatg
      301 tttgatgttc taacgttgac ccgtaatccg ggttgcggac agtgtctggt gggtagtttg
      361 actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct aatcctggtc
      421 ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt gacggcgcga
      481 gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc catcgctcaa
      541 cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca tatcgacggc
      601 ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg tcccaagggt
      661 atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt gagacagttc
      721 ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt acgagaggac
      781 cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc cggtagctaa
      841 atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgcc
//
LOCUS       DAZGOB010000435          878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGOB010000435 DAZGOB010000000
VERSION     DAZGOB010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.438"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>878)
                     /locus_tag="ACHLIF_005535"
     CDS             complement(<1..>878)
                     /locus_tag="ACHLIF_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9609289.1"
                     /translation="LSAYEIACDIEDSEYLDLSTILIADKEFLINLIVRLEQGLSPSL
                     EQENIVQLISVSSVISSLSSLCDWLERKRVIILLDDAAISLTPEYLYEFFDIVRSLKT
                     SKIALKASVYPGTTEYGPRFHVAHDAETIDAWISVQHPNYSSVMDAIAQARFPGYDGI
                     PDDLKELFKFSAFGIPRSFLMMLRDYQTTLSQTTQQKFNKIIERYVELRLSEYSSLKD
                     KLPRLFDIVSLGESLLLKVVELLKESNSQYKEEKQVIIGIEKNDNVYFSRLTKLLIET
                     GLFYNLPDISHGDGRT"
BASE COUNT          294 a          164 c          143 g          277 t
ORIGIN      
        1 taagtacgcc catcaccatg agaaatatca ggaagattat aaaaaagccc tgtttcaatt
       61 aatagcttag tgagtcgaga aaaataaaca ttatcatttt tctctatacc gattatgact
      121 tgtttctctt ctttatattg ggagttactt tccttgagta attcgactac ttttaataaa
      181 agtgattcac ctaatgaaac aatgtcaaac aatcgaggta gcttatcttt tagtgaactg
      241 tattcgctga gtctaagctc aacatacctt tcaattattt tattaaattt ttgctgagtt
      301 gtctgactta aagttgtttg ataatctctt aacatcatca aaaaagaacg tggaatacca
      361 aatgctgaga atttaaataa ctctttcaag tcatccggaa tgccatcata cccaggaaac
      421 cttgcctgcg ctatagcatc catcactgag gaataattag ggtgctgtac cgaaatccaa
      481 gcatcaattg tttcagcatc atgagcaaca tgaaaccttg ggccatattc ggttgttcca
      541 ggataaaccg atgcctttaa agctattttt gaggttttta gactacgtac aatatcaaaa
      601 aactcgtaaa gatattcagg agttaagctt attgctgcat cgtctaataa aataatgact
      661 cttttacgtt caagccaatc acatagtgaa ctcaacgagg atatgacaga agaaacactg
      721 atcaattgaa caatgttttc ttgttctaat gatggcgaaa gcccctgctc aagccttacg
      781 attaaattaa ttaggaactc tttatccgct atgagtatcg ttgacaaatc aagatattca
      841 gaatcttcaa tatcgcaagc aatttcataa gcagagag
//
LOCUS       DAZGOB010000436          870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGOB010000436 DAZGOB010000000
VERSION     DAZGOB010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.439"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..363
                     /locus_tag="ACHLIF_005536"
     CDS             <1..363
                     /locus_tag="ACHLIF_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9609290.1"
                     /translation="KVPAAKITKAYFELGMTFPELYDDSHPEALARNTQKIFRWLDKD
                     TPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYREIVERRDRLVKDVDDFVASAVV
                     LYDQMNRGGPAGNAVVMH"
     gene            386..>870
                     /locus_tag="ACHLIF_005537"
     CDS             386..>870
                     /locus_tag="ACHLIF_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9609291.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     P"
BASE COUNT          226 a          194 c          255 g          195 t
ORIGIN      
        1 aaagtaccgg ctgcgaaaat taccaaagcg tatttcgagc tgggaatgac gttcccggaa
       61 ctgtatgacg acagccatcc ggaagccctg gcccgtaata cccagaaaat tttccgttgg
      121 ctggataaag acacccctga tgctgttgaa aaaatgcagg ctctgttacc ggcgatcgaa
      181 aaggcgatgc cgcctttgct ggtggcccgt atgcgcagcc acagttctga atattaccgt
      241 gagatcgtcg aacggaggga tcggctggtg aaggatgtcg atgattttgt tgcgtcagcg
      301 gttgttttgt atgaccagat gaatcgcggc ggcccggcag ggaatgctgt ggtgatgcac
      361 taaaagcacg gtgttcgggg gttttatgag cagcaagctt catggtcttg tctgggaagg
      421 gtgcgccttc accggcatga tcttatccag ggtggcggtt atggcccgtc ttgcagacta
      481 cagcaatgac gagggcgtgt catggcctgc cattgaaact atccggcgtc agatcggtgc
      541 aagaagtgaa tccacagtga aatcggctat tgcagaactg gcgaaagagg gctggctgac
      601 gaaggaagag cgtaaggtcg gtgggcgtaa tgtaagcaat atctatcggc ttaatgtgga
      661 aaaactcgaa gcagctgcgg cggcggcgcg tgagtcatat aaaccgaaaa gaaaaattag
      721 cccggcaaaa aatgacccgt taacagttga cccgtcaaat attgccccct caacggttga
      781 cccgtcaaat tttgatggat caactgttga taacaaactg ccgattaggg gggcgatgat
      841 tgaccccgat ccgtcagtat taaaacctga
//
LOCUS       DAZGOB010000437          863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGOB010000437 DAZGOB010000000
VERSION     DAZGOB010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.440"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>863)
                     /locus_tag="ACHLIF_005538"
     CDS             complement(<1..>863)
                     /locus_tag="ACHLIF_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9609292.1"
                     /translation="TRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNT
                     VGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSIT
                     DVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGA
                     GIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITS
                     GGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIV
                     KTVGKAFPRDSR"
BASE COUNT          185 a          239 c          196 g          243 t
ORIGIN      
        1 cgggagtccc gcggaaaagc cttacccact gttttgacta tatcgccttc aatctggttg
       61 gctgacagtt tccccttaat ctgacagttc tcattaattg tgacattgtt gagcgtcccg
      121 gcgttcgcat tcacactgcc actgatatct gcatttttag cggtcagctt tccgtccggt
      181 gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt caggcgtttc
      241 aggaacacgt cgttcatgaa tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg
      301 ccattcgcag gattaatcat cgcaatcctg tctgccgcca gcagcacctg actctgcatt
      361 cctgctggcg tattctcaat accggcaccg atacccgcaa tataaaggcg tccgtccttc
      421 atctgttgca gcttcacagc ccacatgctg ttcagattat tatttgtatc aacctgaacc
      481 ttctgtatct gctggatcgc tgcactctgg tcttccagtt tcttattgac ggtctgcgtg
      541 atttcattac tgacatccgt gatggacgtt ctgatttccg ccaggtcagg cgcaagctga
      601 ccgttatcaa tctgagtcca caactcctga gccagatggg ttttccctat ctctcctttg
      661 aaaaaatcca gatagccgga tgcatcatca ctcggctggc caacagcctc cacaaatgcc
      721 gatttgccaa cggtgttcac actgcggata taaaagtaat aatcatggcc cggtttgata
      781 ttgatactgg cggctatcca gtacagcccc gtgccaaggt agcgggctgt ggtttcaacc
      841 tgcctgatat cggtaatccg cgt
//
LOCUS       DAZGOB010000438          853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZGOB010000438 DAZGOB010000000
VERSION     DAZGOB010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.441"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(393..>853)
                     /locus_tag="ACHLIF_005539"
     CDS             complement(393..>853)
                     /locus_tag="ACHLIF_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9609293.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRA
                     QAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          206 a          225 c          228 g          194 t
ORIGIN      
        1 ggtgttcaga tgtcgtttca tcagcttttc cttacagagg gtgaataaaa aagccggtac
       61 ccacaaatgt ggataccggc aggtacaaaa cagcatgctc agataaagtg gtttttctcg
      121 gacataaatg cttcccctct ctcatccgcc agagaagtat cagtccgttg tgtgcaattc
      181 cgttctgcat gatgaagaga ttcagctgcc ttttcgcaga ccattaacca ctataaacga
      241 tcgatagcaa cgatcgatac ctgttttatc gatcgtttta ttctattaac gacaaggcgg
      301 tcacgcaaga gaaacaggag gagtatgagg ggtttgtgtc atgttttcag ttggttaacg
      361 taatgttatc aggcatttcc ctggcagact gttcagaaga aaatccagtt ccatacagcg
      421 cgggcaacag agaccaccgt gtcacggacg gcacgcagaa ccgtacgggc aacagaggca
      481 acacagacgc tctccgccgt gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg
      541 gcctgagcgc ggacagattc cgtgcgcagc tcgtcctgca ggcgggtcat caggggactg
      601 gcggcatggt cgggaagtgc tgtcattagc gcactgacca gcgtatccag ttcccagccg
      661 gtgcgggccg atacggcaac aaccggatgt acgggccgga acagacggaa taccgcctcc
      721 gttttctccc gaatgttctg tgcctgtgcg ggagaaggct gaatgccggc catatcccat
      781 tcatggcagg gctccgtttt gtcggcctgc gtcaccacaa acagcacctg ctgatgtccc
      841 cggtgcagga tgt
//
LOCUS       DAZGOB010000439          850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGOB010000439 DAZGOB010000000
VERSION     DAZGOB010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.442"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            63..266
                     /locus_tag="ACHLIF_005540"
     CDS             63..266
                     /locus_tag="ACHLIF_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO9609294.1"
                     /translation="MSNLFLTEDELLILTGCKYASHQRKWLTENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            266..>850
                     /gene="xerC"
                     /locus_tag="ACHLIF_005541"
     CDS             266..>850
                     /gene="xerC"
                     /locus_tag="ACHLIF_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO9609295.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAAR"
BASE COUNT          286 a          179 c          189 g          196 t
ORIGIN      
        1 ttaatgttca accctaattg ccgctaaccg tatatagtta gcggcggtta tgagatatag
       61 ctatgagcaa cttattttta accgaagatg aattgctaat attaacgggc tgcaaatatg
      121 caagccacca gcgaaaatgg ttaacggaaa acgggcttcc gttctatacc aatcgtagtg
      181 gcaaaccgat tgtcagccgg gatctattta cctgcaataa aactttacca ccacgcgagg
      241 tagagccgaa ttttggtgca atctaatggg aagacgaagg aaaaatcctg aacacgaaaa
      301 actacctcca aatgtatacc caaataaata tagttatgta tggaaaccaa catccagaga
      361 atctgtcaca ctaaccgcca tcaaggatgg tttagctgct ttatggaaaa agtatgagga
      421 aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc ttgtgggaaa aattcctcgc
      481 cagcgcctat tacagtgacc tcagtccaag aacacaaaaa gattatctgc aacatcaaaa
      541 aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc ataaaaccag aacacatccg
      601 tcgatacatg gacaagagag gggaacagag taaaacgcaa gccaaccatg aaaaaagcag
      661 tatgtcccgc gtttacagtt gggggtatga gcgagggtac gtgaaggcta acccatgtgc
      721 aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat gtaaccgaca aagaatacca
      781 ggcagtatta agcgttgcac ctcttcctgt ttttatcgca atggaaattg cctatctgtg
      841 tgcagcgagg
//
LOCUS       DAZGOB010000440          845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGOB010000440 DAZGOB010000000
VERSION     DAZGOB010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.443"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>845
                     /locus_tag="ACHLIF_005542"
     CDS             <1..>845
                     /locus_tag="ACHLIF_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9609296.1"
                     /translation="GQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALT
                     KTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAWRNNEANKP
                     KSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKTLTEMQKQE
                     LLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARERMRELQQI
                     RDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAESDVQRADWQ
                     KGLQEG"
BASE COUNT          232 a          195 c          281 g          137 t
ORIGIN      
        1 ggtcagcata cggaattcaa ccgtctgctg gggctgggga atgaactgct tcagcagcgt
       61 caggggctgg tgaatgtacc gttacggctg ccacaggcca ccctggatga taaacagcag
      121 agtgccctga caaaaacaga gcgtgagctg gccctgtccc gactgaaggg ggaggaaaaa
      181 gagcgtgccc ggctggggta tgcggcggat gaccttggtt ttgtggggga ttcgtatcag
      241 gaggcgagac agcgttatat cagtaatgcc ctggaagcct ggcgtaataa cgaggcgaac
      301 aaacctaaat cccggggtgg aaaatcagag acggaaaaag cggaagacag tttttcccgg
      361 ctactgaagc agcagaaaga gcaactggca ctggcgggtc agaatacaga gctggcgaag
      421 ctgaagtacc agactgcgca gggcgaactg aaaaccctga cggagatgca gaagcaggaa
      481 ctgctgcgta acgcggccct gattgaccag caaaaaatcc gggaacagtt gcgatcccgg
      541 gaagagaccc tgaagaatga taatgtggct gcgcgtgcat caaatgaagc cgaactgctg
      601 ggatacgggc agggagaacg agccagggaa cgcatgcggg agttgcagca gatccgcgac
      661 agcttccgcc agaaggatgc ggaccttcag tctcagtatc agaccgggga tatcagtgag
      721 gatttttaca gacaggcacg ggcacagaac gcgcagtatc tgagcgaacg ccttaaggac
      781 caggcagcct tttatgccga atcggatgtg cagcgtgcgg actggcagaa agggctgcag
      841 gaggg
//
LOCUS       DAZGOB010000441          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGOB010000441 DAZGOB010000000
VERSION     DAZGOB010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.444"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..746
                     /locus_tag="ACHLIF_005543"
     CDS             <1..746
                     /locus_tag="ACHLIF_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO9609297.1"
                     /translation="VSVINPALGKAFAQSEGLRNKTDTVDARMLAEFCRQKRPAAWEA
                     PHPLERALRALVVRHQALTDMHTQELNRTETAREVQRPSIDAHLLWLEAELKRLEKQI
                     KDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRY
                     ESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEWGRAFRDRLAANGKKGKVILGA
                     MMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          204 a          205 c          267 g          158 t
ORIGIN      
        1 tagtgtcagt cattaatcct gcgctgggta aagctttcgc tcagagtgaa ggactgcgta
       61 acaagactga taccgtggat gcgcgcatgc tggcagagtt ctgtcgtcag aagcgccctg
      121 cagcctggga agcgcctcac ccgcttgaac gcgcgttgcg tgccctggta gtccgccacc
      181 aggcgctgac agatatgcac acgcaggaac tgaatcgcac tgaaacggcg cgggaagtcc
      241 agagaccgag cattgatgct caccttctgt ggcttgaagc agagctgaag cgtcttgaga
      301 agcagataaa agacctgaca gacgatgatc cggatatgaa acaccgcagg aaactgctgg
      361 aaagcatccc gggtatcgga gagaaaacat ctgcggtatt gctggcttat atcggtctga
      421 aggaccgctt cgcccatgcc agacagttcg ccgcttttgc gggtctgaca ccacggcgtt
      481 atgaatcagg tagcagtgtg agaggggcga gccggatgag taaggccgga catgtgtcgc
      541 ttcgcagggc gttgtatatg cccgcaatgg tagccaccag taagactgag tggggacggg
      601 cgttccgcga ccgtctggcg gctaatggca agaaaggaaa ggtgattctc ggcgcgatga
      661 tgcgcaagct ggcacaggtg gcgtatggag tgctgaagtc aggcgtgccg ttcgatgcgt
      721 cacggcataa tccggtagcg gcgtaaaaat cgcggaaggg atgaaaaaaa cagcgcctga
      781 cggcgctgtg tctggcatgc ctgcaatccg ggaaaccgga ccaggaaaaa actt
//
LOCUS       DAZGOB010000442          824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGOB010000442 DAZGOB010000000
VERSION     DAZGOB010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.445"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(59..604)
                     /locus_tag="ACHLIF_005544"
     CDS             complement(59..604)
                     /locus_tag="ACHLIF_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO9609298.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
BASE COUNT          193 a          234 c          197 g          200 t
ORIGIN      
        1 ctgtggctcc ggcctgaaca gtgagcgaag cccggcgcgg acaaaatgcc gcagcctgtt
       61 aacctgactg ttcgatatat tcactcagca accccggtat cagttcatcc agcgcggctg
      121 ctttgttcat ggctttgatg atatcccgtt tcaggaaatc aacatgtcgg ttttccagtt
      181 ccggaaaacg ccgctgcacc gacaggggga tcccgtcgag aatactggca atttcacctg
      241 cgatccgcga cagcacgaaa gtacagaatg cggtttccac cacttcagcg gagtctctgg
      301 catttttcag ctcctgtgcg tcggcctgcg cacgcgtaag tcgatggcgt tcgtactcaa
      361 tagtccctgg ctggagatct gtctcgctgg cctgccgcag ttcttcaact tcccggcgca
      421 gcttttcgtt ctcaatttca gcatcccttt cggcatacca ttttatgacg gcggcagagt
      481 cataaagcac ctcattaccc ttgccaccgc ctcgcagaac gggcattccc tgttcctgcc
      541 agttctgaat ggtacggata ctcgcaccga aaatgtcagc cagctgcttt ttgttgactt
      601 ccattgttca ttccacggac aaaaacagag aaaggaaacg acagaggcca aaaagctcgt
      661 tttcagcacc tgtcgtttcc tttcttttca gggggtgttt taaataaaaa cattaggtta
      721 cggcgaagaa gaacggaaac gccttaaacc ggaaaatttt cataaatagc gaaaacccgc
      781 gaggtcgccg ccccgtaacc tgtcggatcg ccggaaagga cccg
//
LOCUS       DAZGOB010000443          820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGOB010000443 DAZGOB010000000
VERSION     DAZGOB010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.446"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>820
                     /locus_tag="ACHLIF_005545"
     CDS             <1..>820
                     /locus_tag="ACHLIF_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9609299.1"
                     /translation="YNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKK
                     LLPYIPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIART
                     KAALQKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGS
                     AADVPWICGDTTYYWKNTYGTQYNTIYGAYKNRESEGVYFVPFMTDGNGVNTATNAPA
                     EDPDIPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRMATAILNAAGRTS"
BASE COUNT          199 a          211 c          240 g          170 t
ORIGIN      
        1 gatataacga tattattccg gccgaccact gcctgcatga tgtgcaggat atgagtacgc
       61 tgaatcatcc gaaggcagac ctgagcaaag ggcagtacgg ctgtgtcggc caggggttac
      121 atattgccaa aaaactgctc ccgtatatcc cgaataacgc ggggatcctg ctggtaccat
      181 gctgtcgtgg tggttcggca tttacccagg gcgcggaggg gacattcagt gcggacacgg
      241 gggccagcca ggattcggcg cgctggggtg tgggtaaacc gttatatcag gacctgattg
      301 cgcgcaccaa agctgcatta cagaagaacc cgaaaaatgt gttgctggcg gtgtgctgga
      361 tgcagggaga gtttgacatg agcgccgcca cccacgcaca gcaacctgcg ctgtttacag
      421 ccatgctgac acagtttcgt gctgacctct ccgtgtttaa cgcgcagtgc catggtggca
      481 gtgctgcaga tgtgccgtgg atttgtggtg acacgacgta ttactggaaa aatacctacg
      541 gcacccagta caacaccatt tacggggcgt acaaaaacag ggagagtgag ggcgtttatt
      601 ttgtgccctt catgacagac ggtaacggcg tcaataccgc cactaacgcg ccggcagaag
      661 atccggatat tccggcatca ggatattacg gtgcggcatc gagaacgaat ggaaaccagg
      721 tatcatcaaa ccgcccgaca catttcagtt catgggcgcg caggagcatt attccggatc
      781 gtatggcaac cgctattctg aacgcagccg ggcgcacctc
//
LOCUS       DAZGOB010000444          816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGOB010000444 DAZGOB010000000
VERSION     DAZGOB010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.447"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..197)
                     /locus_tag="ACHLIF_005546"
     CDS             complement(<1..197)
                     /locus_tag="ACHLIF_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609300.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTV"
     gene            complement(261..>816)
                     /locus_tag="ACHLIF_005547"
     CDS             complement(261..>816)
                     /locus_tag="ACHLIF_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO9609301.1"
                     /translation="GRDRETREWLGWGHAWAHETAVVRRKSEASRFQDLVACGDMTIV
                     RRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWK
                     LGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMA
                     LFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT          178 a          272 c          200 g          166 t
ORIGIN      
        1 accgtacact gccccaccgg gcgggaatgg tcatgctgcc agagaaacgg tatcgcactg
       61 ccaaactccg cgccctccgg ctccaggatg tcaccatccc gatccggaga aggcgttgac
      121 gcaatcccgg tgatcacccg ttcatcctca ctgaaggatt tcaccgtcag cagggaacag
      181 gcccgtttaa gagtcacatc agcctcctga aaataaaaaa accgccggag cggttcgtga
      241 tggttacagt gtgaacaggg ttatatgaaa aaaaccgcat attctttctt tttcggttcc
      301 gggttaaggg acatcaggga gaccgcattg aacagcgcca tcagcgggtc aatttttccc
      361 cgtccactgg cctgtttggt gataagaatg gcgttacctt taggctccac ccgggcattg
      421 ccgacacacc aggccatcag gggctggtca ccatgcacca gcaccccttc agccagtttg
      481 cgctcggtgg ttttaatggc cccgcccagt ttccagccct ggcttatccc caccacaatt
      541 ccgtcgggga tcccggcttc cgccagtgaa tccagaatct gccccacccc tgacgggtca
      601 ataccgatat ggtccagtaa ctcagcctca tgaatgcgac gcacatattc cgccacttcc
      661 gccgtgtcat ccccgacacg ccggacaatg gtcatatctc cacaggcaac aagatcctga
      721 aaccgggacg cctcgctctt ccgtcggacc accgcggttt catgcgccca ggcatggccc
      781 cagcccagcc attcgcgggt ctcccggtca cggcca
//
LOCUS       DAZGOB010000445          806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGOB010000445 DAZGOB010000000
VERSION     DAZGOB010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.448"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            140..649
                     /locus_tag="ACHLIF_005548"
     CDS             140..649
                     /locus_tag="ACHLIF_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9609302.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            621..>806
                     /locus_tag="ACHLIF_005549"
     CDS             621..>806
                     /locus_tag="ACHLIF_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609303.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMN"
BASE COUNT          241 a          161 c          202 g          202 t
ORIGIN      
        1 ggtttctctt caagttaact atatgaaaaa tataaaaaca ggtcttctgt gaaccggaca
       61 tgcacaaaaa atagatatgt aaaccggaca tgaccggttt tgttgtgatt gtgaggtgag
      121 agtttttgcg aggtgaggag tggctacgca gactgaagtt gccaggcatt taagtctgac
      181 cgatcgccag cttcgcagat tgcagaaatt gccgggtgcc ccgatatcaa ataagcgagg
      241 gcaactggat ctggatgcct ggcgcgattt ttacatatcg tatctgagga gaagtaaaaa
      301 cgatgtgcct gatggcgata gcgaagacga ctatgaggaa aaattgctta ttgccagatg
      361 ggaactgaca gcagaacagg ctgttacaca gcagttaaaa aatgaggtgt caaaaggaaa
      421 acttattgac accgggttct gtatttttgc cctcagtaag ctggcaatgg cgttatccag
      481 tacgcttgat tccatccctt tatccatgca acgacagttt cctgatttaa caccgcgcca
      541 tcttgaccat ctgaaaaccc ttattgcgaa gggggcaaat cagtgtgcgc gggcggggga
      601 taaattaccg gatttactcg atgaatatat cagagcaaca actgaataat atgatgagtg
      661 ctgtcacaac agcattacag cccctgataa gggcattgcc ggtgacgcca gttgaatggg
      721 ctgatcaaaa ttattatctg cctaaagaat cttcatatgg tgagggagaa tggaaaacgc
      781 tgccattcca gatcgccatc atgaac
//
LOCUS       DAZGOB010000446          804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGOB010000446 DAZGOB010000000
VERSION     DAZGOB010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.449"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            76..>804
                     /locus_tag="ACHLIF_005550"
     CDS             76..>804
                     /locus_tag="ACHLIF_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxin"
                     /protein_id="HFO9609304.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESKSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYAN"
BASE COUNT          315 a          139 c          145 g          205 t
ORIGIN      
        1 cttggttaag ggtgttaata ataagatttt ctcacttatt aaaacataaa taatttttta
       61 agcggaaatg taattatgag actgccagag aaagttcttt ttcctcctgt cactagtggc
      121 ctgtcagggc aggaaaaaca aaaaaaaccg aagagcatta ccggatttca ggaaaattat
      181 caacgcaata tcaggccaat caaaacagca tcagaagccc gactacgctt ctttgataaa
      241 atggtttcga aagaaaactc tctggaagat gttgtttctt taggtgaaat gattcagaag
      301 gaaatttatg ggcatgaaca aagaacattt tcaccagttc atcatacagg taactggaaa
      361 tcatcattgt tacacaacgc gctccttggt ctggcaaatg tttataatgg cttacgggaa
      421 acagaatacc ctaacacttt caacagagat ggtataaaaa gtactaactc ttttagagat
      481 aacttattga caaaaacaag aactcccaga gataattttg aggaaggaat aaaacatcct
      541 gaacatgcaa caataccata tgacaacgac aatgaaagta ataaattgct aaaagcagga
      601 aagatagctg gtaacaataa cgagctgttg atggaaataa aaaaggaatc caaaagcgac
      661 catcaaatcc ccctgtcaga taagttcctg aaaaggaaaa aacggtctcc tgtagctgaa
      721 gataaagttc aaaactcgtt aacaccagaa aattttgttc agaaaatttc acttagtgat
      781 gagcttaaaa caaaatatgc aaat
//
LOCUS       DAZGOB010000447          804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGOB010000447 DAZGOB010000000
VERSION     DAZGOB010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.450"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            110..>804
                     /locus_tag="ACHLIF_005551"
     CDS             110..>804
                     /locus_tag="ACHLIF_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HFO9609305.1"
                     /translation="MNTNIGMRYIDVVANGNAGEFYFAYWISSNFIWPCRILDIDMGL
                     DAQVEVYDDQNHSTGMFIGVQVKTTATTLEENPNVSVPMKNIAYWESINDPVVIIRTC
                     LNNNSKEPSLYWKHLPKSELRKYLNRAEEKGYETVTIMFEEDKNSLLKTDKSAWLKIF
                     LSDYDKEIIESVVGIKSKLDKLGKYLKDNYIDEQFICGFPSDDFPSELNDTLNKYDEL
                     AVAVKVNPRLKYLS"
BASE COUNT          281 a          104 c          175 g          244 t
ORIGIN      
        1 ggttgtaggt tgtttagttg aagtttaaaa gcagcgcttt agcagttgat tgcgttaaac
       61 cattgtaata tacaaagggg aaatagtatt tagccgtggg ggagtattaa tgaatacaaa
      121 tatcggtatg agatatatag acgtagtggc aaatggtaat gccggagagt tttattttgc
      181 atactggata tcaagtaatt tcatctggcc ttgccgtatt cttgatattg atatggggtt
      241 agatgctcaa gttgaagttt atgatgacca aaatcattca actggaatgt ttattggtgt
      301 gcaggtgaaa acaaccgcga ctacgctaga agaaaaccct aatgtttctg tacccatgaa
      361 aaatatagct tattgggaaa gcataaatga tccagttgtt attattcgga cctgtcttaa
      421 taacaattca aaagagccta gcttgtactg gaagcatctt ccgaaaagtg aattaagaaa
      481 atatttaaat agagccgagg aaaaaggtta tgaaacagta accataatgt tcgaggagga
      541 taagaactca ttgctgaaaa ccgataagtc agcatggcta aagatatttt tgagcgatta
      601 tgataaggaa atcattgaaa gtgtagtagg gattaaaagt aaactagata agttaggtaa
      661 atacttaaaa gataattata ttgatgagca gtttatttgt gggtttccaa gtgacgattt
      721 cccatcagaa ctgaacgata ctttgaataa atatgatgaa ttagcagttg ctgttaaagt
      781 aaaccctcga ctaaaatatc tttc
//
LOCUS       DAZGOB010000448          790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGOB010000448 DAZGOB010000000
VERSION     DAZGOB010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.451"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            118..645
                     /gene="sodC"
                     /locus_tag="ACHLIF_005552"
     CDS             118..645
                     /gene="sodC"
                     /locus_tag="ACHLIF_005552"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO9609306.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          209 a          204 c          187 g          190 t
ORIGIN      
        1 ggtattatta atcgcagtga taggatattg cacagctatt gcgcgatatc atcagaacgc
       61 tgtttgttac cctgtaacca gcaagctcag tctgttaacg gaattaatga gggttttatg
      121 aaatgtaaaa tcattgctgc cattgccatg ctgacagcag catcatgcgg atacgcagca
      181 gaacaggaag tcccaatgaa ccttgtcagt gctgacggaa aagaagtcag cattggaaaa
      241 ataaccattc aggagacccc ctacggtctg ctgttcacac cagcccttca ctctctgtct
      301 gaaggcattc atggttttca tgtgcacgaa aaaggaaatt gcgccccggc actgaaagac
      361 ggaaaaccgg tcgcagcatt atcggctggc ggtcactttg acccgaaaaa caccggcaaa
      421 catcttggcc cctggtctcc ggatggacac ctgggcgacc tccctgcgct gttcgtgacg
      481 catgacggaa aagcgaacta cccggtcctg gccccgagac tgaactcatt aaaagagatt
      541 aaagggcgtt ctctcatgct tcatgctggc ggtgataacc atcatgacca tccggagccc
      601 ctgggcggtg gtggtgcgag aatggcctgc ggcatcattc aataatcagt caggtaaggg
      661 gcgggcccct tacctttatt cctcaggacg ataaatcctt tctccctgaa aagaacggca
      721 catcctccct ctctgagtta atgtttttgt cgtgacataa gaataattcc ttacactcaa
      781 tcttcgtaac
//
LOCUS       DAZGOB010000449          785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGOB010000449 DAZGOB010000000
VERSION     DAZGOB010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.452"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            197..568
                     /gene="ler"
                     /locus_tag="ACHLIF_005553"
     CDS             197..568
                     /gene="ler"
                     /locus_tag="ACHLIF_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO9609307.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEDEIIPLKNI"
     gene            583..>785
                     /locus_tag="ACHLIF_005554"
     CDS             583..>785
                     /locus_tag="ACHLIF_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscE/YscE/SsaE family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HFO9609308.1"
                     /translation="MITITELEDEIIKNKEAANIFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRIIEIMLI"
BASE COUNT          300 a          123 c          155 g          207 t
ORIGIN      
        1 aatgtgtttt acacattata catcggtgat taataacttt tataaggatt ttgtttgatg
       61 aagtagatgt gttctaattt gatagataaa acgttatctc acataattta tatcattcga
      121 ttaattgttg atccttcctg ataaggataa gattgctaat agcttaatat attaaagcat
      181 gcggagatta tttattatga atatggaaac taattcgcac acaacaagcc catacattca
      241 gcttattgag caaattgaag tgttacaaca ggaagcaaag cgactgcgag agcaggaaat
      301 tcaaagtgta attgagtcga ttcaaaagca gattacttat tacaatataa ccctacaaga
      361 gctgggatat actaatgtgc ctgatgatgg ccttgctcgc cggaactcat cgaaaggagt
      421 ttattatcgc aatgaagaag ggcagacctg gtcgggagtt ggccgacagc cacgctggct
      481 taaagaagca ctgttgaatg gaatgaagaa agaagatttt cttgtgaagg acaccgaaga
      541 cgaaataata ccgctgaaaa atatttaaca tgaaatcatt aaatgataac gataactgaa
      601 ctggaagacg aaataataaa aaataaagaa gccgcaaata ttttcattga aaaaataaac
      661 gacaaaaaga acgaaatcca tgaaaaaatg aaacacccct tggataaagt tacctacaat
      721 gaagcaaagg aactgctcat tgcctgcgat gcggcaatca ggatcataga gataatgcta
      781 atcag
//
LOCUS       DAZGOB010000450          785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGOB010000450 DAZGOB010000000
VERSION     DAZGOB010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.453"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..321
                     /locus_tag="ACHLIF_005555"
     CDS             <1..321
                     /locus_tag="ACHLIF_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9609309.1"
                     /translation="REFGECKGKKHYHLMLLVNRDTWCRAGDYRAPGSLAGMIKQAWC
                     SALGVDVGCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKEHTKVHCTGERNFG
                     CSRG"
     gene            487..>785
                     /locus_tag="ACHLIF_005556"
     CDS             487..>785
                     /locus_tag="ACHLIF_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9609310.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHC"
BASE COUNT          174 a          215 c          226 g          170 t
ORIGIN      
        1 cgggagtttg gggagtgcaa aggtaaaaaa cactatcacc tgatgctgct ggtcaaccgg
       61 gatacctggt gtcgtgccgg tgattaccgc gctccgggat cactggccgg gatgattaaa
      121 caggcgtggt gcagtgccct gggagtggat gtcgggtgcc atgccacgct ggtgcatttt
      181 ccggcctggc cggcggtgtg gctggcgcgt aatgatgaca ccggctttca gcaagtgctg
      241 gaacgtgctg actatctggc gaaggagcat accaaagttc actgcaccgg tgagcgcaac
      301 tttggctgca gtcgtggctg agccagacag acgagttatc tgacttcagt gtgatgacgt
      361 gttcacggcc accacactga ttttccctta tgagactgaa ctgaaaccgc cattcacacc
      421 ggaccacgct gcccgtaccg ggacggctgt tgcctgcgtg tcgtctgacg gtaaggaaca
      481 tatactatgt acgcaaaatc ctttctcgct cttgatggca acggacgtct gacgggcgcc
      541 cgtacagcac aggccgcacc ttatgctcac tacacctgcc acttgtgtgg cagtgcactc
      601 agataccatc cgcaatacga cactgaactt ccctggtttg aacacactga cgacaggctg
      661 acagagcacg gtcaacagtg cccttatgtc aggccggagc gcagagaaat acagttgatt
      721 aaacgtctgc agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc cagctggcac
      781 tgcag
//
LOCUS       DAZGOB010000451          780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGOB010000451 DAZGOB010000000
VERSION     DAZGOB010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.454"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            65..>780
                     /locus_tag="ACHLIF_005557"
     CDS             65..>780
                     /locus_tag="ACHLIF_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9609311.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          261 a          135 c          169 g          215 t
ORIGIN      
        1 gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat
       61 tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac
      121 gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgccaaac aaggtgaacc
      181 cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt
      241 tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt
      301 cgtacaggga cgttatgaag gctttggtcc taatggctca atgattattg ctgagacatt
      361 gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg
      421 caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg tgattgtatt
      481 taaaggaaca gaccctgacc atatttttga aattttgctt gaagctgaag ttgatgttcg
      541 tgatgtaact gaagaagaag gtaatattgt tatttatact gaacctactg accttcataa
      601 aggaatcgcg gcgctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat
      661 gattgctcaa tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt
      721 tgatgccctt gaagatgacg acgatgtaca aaaagtttat cataacgtcg caaatctcta
//
LOCUS       DAZGOB010000452          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZGOB010000452 DAZGOB010000000
VERSION     DAZGOB010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.455"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>778)
                     /gene="espL2"
                     /locus_tag="ACHLIF_005558"
     CDS             complement(<1..>778)
                     /gene="espL2"
                     /locus_tag="ACHLIF_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO9609312.1"
                     /translation="LPDNILQGVVINFAMGAGLREIIKKVYNDTRFTDLTKSQMKILC
                     ESKNVNNVPGLLLALQNGHDNVIDEYGILIKKSNLNKEELIHILSARTLDGTIPGLYQ
                     ALQNGHAQAIKSYGNLVLDTIDKNIDLEYLLSAFKYEAHSSNKYTPGLFSAFQNGHAD
                     AIKAYCGVLGNSNLKRGEIIRMLEARNYDGAPGLLLAYQNGDINTIQSFFDSLIMLDI
                     SKDFIEELLTAKHYDFTGLSLAISHRHDHVVKLYGKLFKKL"
BASE COUNT          258 a          143 c           99 g          278 t
ORIGIN      
        1 agttttttaa ataactttcc gtataatttg acaacatgat catgtctatg actgatcgct
       61 aatgataacc ccgtaaaatc ataatgtttt gcagtcaata actcttcgat aaaatccttt
      121 gatatatcta acattattag tgagtcaaaa aaagactgaa tcgtattaat atctccattt
      181 tgatacgcaa gaagtaatcc tggtgcacca tcataatttc tcgcctcaag catcctgatt
      241 atctcacctc ttttcaaatt actattgcca agtacgccac aatatgcctt tattgcgtct
      301 gcatgaccat tttgaaatgc agaaaataat ccaggagtat atttattaga gctatgtgcc
      361 tcatatttaa atgctgaaag caaatactct aaatctatat ttttatcaat agtatccaga
      421 actaagttac cataactctt aatagcttga gcatgtccat tttgaagggc ctgatataaa
      481 ccaggtatgg ttccatccaa agttcttgca gataatatat gtattaattc ttctttatta
      541 agatttgact ttttaattaa aataccatat tcatcaatta cattatcatg tccattttgt
      601 agagctaaaa gcaacccagg aacattatta acattttttg attcacacaa gattttcatc
      661 tgagattttg taagatctgt aaatcgagta tcgttatata ccttttttat tatctctctt
      721 agtcctgctc ccatagcaaa attaataaca acaccttgga gaatattatc aggtaatt
//
LOCUS       DAZGOB010000453          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGOB010000453 DAZGOB010000000
VERSION     DAZGOB010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.456"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            7..618
                     /locus_tag="ACHLIF_005559"
     CDS             7..618
                     /locus_tag="ACHLIF_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFO9609313.1"
                     /translation="MFSPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQ
                     SNFMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVI
                     GKETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGVDPITRFN
                     TQTRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
BASE COUNT          251 a          140 c          174 g          213 t
ORIGIN      
        1 tattgtatgt ttagtccaat gacaatggta ggtagatcgt tagctcaggc ggctacacaa
       61 actcttagac ctgcggtcac cagagctgcg acacaagcgg gggtgggagc cagtggcatg
      121 aagttcgtgc ccatgcagtc gaactttatg attaatcatg gcaaactgac taaccagcta
      181 cttcaggccg tggccaaaca aacccgtagc ggtgatactc agcaatggtt caaacaggag
      241 caaattactt atatatccag aacagtaaac agagctattg atgactattg catgagtaat
      301 aattcggtaa ttggtaagga aaccaaaagc cacattttca aggctgttga aagcgcgata
      361 aagcagcctc tcgatatgaa ttgtgcacaa acgtcgattg gccatttctt acagtccaac
      421 aaatacttta ataaaaaagt tgatgagcag tgtggcaaag gggtggatcc aattacacgc
      481 tttaacaccc agaccaggat gatagaacaa gtctcacaag aaatctttga acagaaattt
      541 ggtggtttga aagttagtga tatcaaagct ctgactcaga gcacgattgc acagcatgta
      601 caggatactc ggttgtagga ataacctgtt atatatgcgt gttgggcata agttaagctt
      661 cctggtgtat agcgtagtct ttgctgatgt aagaaaaggc tatatacgga aacttgatat
      721 actataaact tcattattta ttatattgaa aaagttattt aattgatttg ttttgtaa
//
LOCUS       DAZGOB010000454          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGOB010000454 DAZGOB010000000
VERSION     DAZGOB010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.457"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..584)
                     /locus_tag="ACHLIF_005560"
     CDS             complement(<1..584)
                     /locus_tag="ACHLIF_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9609314.1"
                     /translation="MQDIHEETLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFD"
     gene            complement(584..>778)
                     /locus_tag="ACHLIF_005561"
     CDS             complement(584..>778)
                     /locus_tag="ACHLIF_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609315.1"
                     /translation="VTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGW
                     SARVGMLRVEFSAEFKQVVN"
BASE COUNT          163 a          291 c          174 g          150 t
ORIGIN      
        1 ttgtcgaact catccgccac cgcaggaccg ttatacccgc attcatctcc ccggtaatcc
       61 cacatacagg tgttcgccag catgatgcga ccaggaaaca gcgccccgtc cgtctcggtc
      121 ggtgtcgcca gcacaaacga ggccgtcatg gccgtcagct ctgacatctg ctccaccacc
      181 cagcggtcgc tcagctcctg ctccgggtcc gcctccggat tgcccgcaac gaaattcacc
      241 gcatccagaa aacgcgcata cacccggcgg cggaccaccg tggcacccac caggctctgc
      301 aaatcctccg ccatcccggt gacaaggccg aaaagattgg acactgtcag cgacgggcgg
      361 gcactgctgc cctttccgtt catctcaaag ccactcccct caatcgggta tgcctgatat
      421 tcacgcccct gccaggtcac cggctcccct ttttcattca gctcattgca gaaaaaatac
      481 cgctcaccgc cctgcaccgt caggtcgatt tcccagagca ccacccgcgg tgactgctct
      541 gacttaaccg actcgttaag agtttcttcg tgaatatcct gcatcagttc accacctgct
      601 taaactccgc gctgaactca acgcgcaaca tcccgacccg cgcagaccac ccggcacagg
      661 tcacctttat ctgccggtat gcatagggtg gcttccacaa aaatgccttc cagcccccgt
      721 gctctgccag aaacgcttcc agatgccggg cctcctcccg ggtcacggaa agcgtcac
//
LOCUS       DAZGOB010000455          770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGOB010000455 DAZGOB010000000
VERSION     DAZGOB010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.458"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHLIF_005562"
     CDS             <1..198
                     /locus_tag="ACHLIF_005562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609316.1"
                     /translation="FNNHDGNVIIVNTSSRFELRKKYVGTLKINSGVSTVDPHWPPVS
                     KRIKLIFFSPTLHSFSCIGVS"
     gene            234..>770
                     /locus_tag="ACHLIF_005563"
     CDS             234..>770
                     /locus_tag="ACHLIF_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609317.1"
                     /translation="MSRRKVGNMGRGDFDRLCNASGLIYNSSSEDDSGGWDAIVEFPL
                     LGGIALLNSLNEQPIQCLVQIKSSDGEKKGVQVKLSNMKRFCDTPLPCFFFFARYNKN
                     ANIESAYLVHINSDIMFQVLKRIRQNDIGKKSPLHKLSFTVNYSDEDEIDLTNEYALK
                     NAIEKHIPHGMKKYSEDKL"
BASE COUNT          242 a          132 c          162 g          234 t
ORIGIN      
        1 tttaataatc atgatggtaa tgtgattatt gtgaatacga gctcaaggtt tgagctccgg
       61 aaaaaatacg ttgggacgtt aaaaatcaac tcgggagtat ccacagttga tcctcattgg
      121 ccccctgtaa gcaagcgcat caagctaata tttttcagcc cgacacttca tagtttttcc
      181 tgcattgggg taagctaaac ctagtatcgt acatatgaaa gagggatgag tttatgagtc
      241 gaagaaaagt tggtaatatg ggacgtggtg attttgacag gctttgcaat gcatctggct
      301 tgatttacaa cagctcctct gaggatgatt ccggagggtg ggatgccatt gtcgaatttc
      361 ctctgttagg tggtatagcc ttactgaata gtttgaatga acagcctata caatgtttag
      421 tgcagataaa gtcttctgac ggagaaaaga aaggtgtaca ggtcaaactt tctaacatga
      481 aacgtttctg tgacacacct ctaccctgtt ttttcttttt tgcgcgttat aataagaatg
      541 ctaatatcga atcagcttac cttgtccaca ttaatagtga tatcatgttt caggttctga
      601 agcggattcg tcaaaatgat ataggaaaaa aatcaccact tcacaaactt tctttcacgg
      661 taaattactc tgatgaggat gagatagacc tgactaatga atatgcactc aaaaatgcta
      721 ttgaaaaaca tattcctcat ggaatgaaaa aatatagtga agataaattg
//
LOCUS       DAZGOB010000456          768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGOB010000456 DAZGOB010000000
VERSION     DAZGOB010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.459"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>768
                     /locus_tag="ACHLIF_005564"
     CDS             <1..>768
                     /locus_tag="ACHLIF_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFO9609318.1"
                     /translation="SSLPDTDKIGGILVKKYLSGSLFSKFRQDTIIPEALSTLQISGP
                     DLIQRKMLQFFRSRGVLGEEFINERKLSDKAYIGVYKTTGTGKYDWLNPESIGVNDVT
                     PADESTWCIGKGRCVDDFLFKDVSKLKTENLPELFLTKIDTDTFFSQWSTKTKKDLQK
                     KIQDLTVRYNELIDSSTIDFKNLYEIDQMLHMIMLEMNDDIAKRSLFSLQVQIAEKIR
                     RMTIPVDNIINIYPDLHKKNDNDLSMSIKGFLASNPH"
BASE COUNT          278 a          132 c          145 g          213 t
ORIGIN      
        1 tgtcctcact tccagataca gacaaaattg gagggattct ggtaaaaaaa tacctttcag
       61 gaagtctttt ttcaaaattc agacaagata ctattatccc ggaggcattg agcaccctcc
      121 agatatcagg tcctgacttg attcaaagaa agatgttgca atttttcagg agtagagggg
      181 tgttaggtga agagttcatt aatgaaagaa aactgagtga taaagcttat attggtgtct
      241 acaaaacaac tggcacaggg aaatatgact ggttaaaccc tgaatcgatc ggcgttaatg
      301 atgtcacgcc cgcagatgaa agtacctggt gtataggaaa aggccggtgt gttgatgact
      361 tcctgttcaa agatgtttca aaactaaaaa cagaaaatct tccagaatta ttcttaacaa
      421 aaatagatac tgatacgttt ttctctcagt ggtcaaccaa aaccaagaaa gatctgcaaa
      481 aaaaaataca agaccttact gtacgttata atgagctaat tgactcgtcg actatcgact
      541 ttaaaaatct atatgaaata gatcaaatgc tccatatgat tatgctagag atgaatgatg
      601 atatagccaa aaggtcattg ttttcattgc aagttcaaat agccgaaaaa attcggagga
      661 tgaccattcc tgtagacaat ataattaaca tctatcctga tctacataaa aaaaatgaca
      721 atgatctgag tatgtccata aaaggctttc ttgcgagtaa tccacata
//
LOCUS       DAZGOB010000457          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGOB010000457 DAZGOB010000000
VERSION     DAZGOB010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.460"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>767)
                     /locus_tag="ACHLIF_005565"
     CDS             complement(<1..>767)
                     /locus_tag="ACHLIF_005565"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9609319.1"
                     /translation="LLVIAFHFRVYLNGVYAQKDLGDILFGIGEVGVDIFFVISGFII
                     TYSSMNKAKNRPMVFAAKRFFRLYPVYFIILTLAIYLNYEEIYTSSQIIKSYLLIPID
                     YNFIGPWSGYSIIIPAWTLTYEIWFYVVFFFSLCISHKYRTAIASFLLIIICVLGQLY
                     FHGDVFLNPIAQPPSGDGIIRNLVFVSNPIIYDFILGMIIAEAYIRLPSRLINKMPVV
                     QFLNITLAIALLFLCLGLRNGPGIVAWGAYSFMIIAP"
BASE COUNT          290 a          141 c          127 g          209 t
ORIGIN      
        1 attggggcta taatcataaa tgaataagcc ccccatgcta caattccagg tccattcctt
       61 aaccctagac ataaaaaaag caaggcaatc gctaatgtaa tattaagaaa ttgaacaaca
      121 ggcattttat ttataagtct agaaggtagc ctaatgtagg cctctgcaat aatcatcccc
      181 aaaataaagt catatattat tggatttgat acaaatacca gattacgaat aattccatca
      241 ccagatggcg gttgcgcgat aggatttaga aaaacatctc cgtgaaagta cagttgacca
      301 agaacgcaaa taatgatgag aagaaatgat gcgatcgcag ttctatattt atggctgatg
      361 cataatgaaa aaaagaaaac cacatagaac caaatttcat acgttagtgt ccatgcaggt
      421 attattatac tataacctga ccatggccct ataaaattat agtcaattgg tatgagcaag
      481 tagctcttta ttatttgtga tgatgtatat atttcctcat agttgaggta tatagccaga
      541 gtaaggatta taaagtaaac agggtacaac ctaaaaaaac gcttagcagc aaacaccatt
      601 ggccggtttt ttgccttatt cattgaagaa taagtaatga taaatccact tataacaaag
      661 aatatatcaa caccaacctc acctatacca aaaagaatgt cgcccaagtc cttttgggca
      721 tacactccat ttaaatatac tctaaagtga aacgcaatga ctagaag
//
LOCUS       DAZGOB010000458          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGOB010000458 DAZGOB010000000
VERSION     DAZGOB010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.461"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>767)
                     /locus_tag="ACHLIF_005566"
     CDS             complement(<1..>767)
                     /locus_tag="ACHLIF_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9609320.1"
                     /translation="RITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTV
                     GKSAFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNTSRLDEFSKEWK
                     DANDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGN
                     ETPMFVAQGNQIFMNDVFLKRLTAPTITSGGSPPAFSLTPDGKLTAKNADISGSVNAN
                     SGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFP"
BASE COUNT          163 a          219 c          179 g          206 t
ORIGIN      
        1 gggggaaagc tttgcccact gttttaacga gatcgccttc aatctggttc gcggacagtt
       61 ttcccagaac ccgacagttc tcattaatcg tgacgttgtt gagcgtcccg gagttcgcat
      121 tcacactacc actgatatcc gcatttttag cagtcagctt tccgtccggt gtcagggaaa
      181 atgccggagg actgccacca ctggtaatgg tgggggccgt caggcgcttc aggaacacgt
      241 cgttcatgaa tatctggttg ccctgcgcca caaacatcgg agtttcattc ccgtttgccg
      301 ggtcaataaa cgcgatacgg ttagcggcaa ccagaaactg gctcagcttg ccttcctccg
      361 tgtcctccat gctgaggcca atacccgcga cataatgttt gccgtctttg gtctgctcaa
      421 ttttgacgcc ccacatggca ttccatttat cgttagcgtc cttccactct ttcgaaaact
      481 catccagtct gctggtgtta tcctccgtca gatcgacttt ttccagcagc tccttgccga
      541 gatgagattc ggttatcttg cctttgaaaa aatccagata accttccgca tcatcgctcg
      601 cccgaccgac ggcctccacg aatgccgatt tgccaacagt gttcacactg cggatataaa
      661 agtaataatc atggcccggt ttgatattga tactggcggc tatccagtac agccccgtgc
      721 caaggtagcg ggctgtggtt tcaacctgcc tgatatcggt aatccgc
//
LOCUS       DAZGOB010000459          761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGOB010000459 DAZGOB010000000
VERSION     DAZGOB010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.462"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..441
                     /locus_tag="ACHLIF_005567"
     CDS             <1..441
                     /locus_tag="ACHLIF_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HFO9609321.1"
                     /translation="ALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFKRHIRHRE
                     ADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYDLRYGHPI
                     NADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            419..>761
                     /locus_tag="ACHLIF_005568"
     CDS             419..>761
                     /locus_tag="ACHLIF_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609322.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVKLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRVTK"
BASE COUNT          223 a          155 c          211 g          172 t
ORIGIN      
        1 gcgcttcgtg aggcagcaca aaactatcag tcgacgctgg cgtggtatca ggctaccccg
       61 gacagcccaa atgctgaacg ggattgtgat gcggctcttg ctgcgtttaa gcgtcatatc
      121 cgtcatcggg aagcggatat tatcgctggt ttgctggatg gactggaaga agcaaaatca
      181 caactcaacg agcagcgtga gtattacgaa ggcgttatct ctgatgggag caagcgtatt
      241 gctgaactgg atgcgcggga agttcaatta ccgactcgct acgaccttcg atatggacac
      301 ccgataaatg cagatgagcg acatgtcatg atacctaaag aaaatggcag ttggctttac
      361 ctgattgacc tagaacacgc attacgcgtc gctggcattc gcatcaaagg agaggagcat
      421 ggaaataaaa ccagaggatg agttaagcaa tatcgtttta tttccggtaa aagaggatga
      481 ccctcgtaat caggttaatt ttctttatga gccatcggaa agaccatatt gtcatcacgc
      541 ctctgtccgg gttgacgaaa aagagcgtca ggtccgctgt aaaatctgcg gtgcagttgt
      601 ggagccattt gactggatgc tctctgtggc gaaaagagaa actaggctgg cagatgatgt
      661 aaagctattg cgccaggagg aacaggaaag gcggaaaaat atagaaaagt taattcagat
      721 tgagcgtaac gcgaaagcgc ggatacgcag ggtgacaaaa t
//
LOCUS       DAZGOB010000460          761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGOB010000460 DAZGOB010000000
VERSION     DAZGOB010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.463"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>761
                     /locus_tag="ACHLIF_005569"
     CDS             <1..>761
                     /locus_tag="ACHLIF_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9609323.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVRDKLDTRRGS
                     HSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPARE"
BASE COUNT          179 a          204 c          233 g          145 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaagccgaa gcatgaggtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cgcagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcatgcag aacatgcgtg acgaagagct ggccattgcc
      181 caggtcgaag agatgcaggc agtttctgcc gtgcttaagg gcaaatacac catgaccggt
      241 gaagcctttg atccggttga ggtggatatg ggccgcagtg cggcaaacaa catcacacag
      301 tccggtggca cggagtggag caagcgtgac aagtccacgt atgacccgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggcgtg gtgaatatca tcgtgtttga tccgaaagga
      421 tgggcgctgt tccgttcctt caaagccgtc agggataagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcaaag cggtgtctta taagggaatg
      541 tatggcgatg tggccatcgt cgtgtattcc ggacagtacg tggaaaacgg cgtcaaaaag
      601 aacttcctgc cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgtacc
      661 tatggctgta ttcaggatgc ggacgcacag cgcgaaggca ttaacgcctc tgcccgttac
      721 ccgaaaaact gggtgactac cggcgatccg gcgcgagagt t
//
LOCUS       DAZGOB010000461          753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGOB010000461 DAZGOB010000000
VERSION     DAZGOB010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.464"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            27..617
                     /locus_tag="ACHLIF_005570"
     CDS             27..617
                     /locus_tag="ACHLIF_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9609324.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          233 a          161 c          169 g          190 t
ORIGIN      
        1 acaccaattt cgtatagagt ctcactatgt ctcgaatttt tgcttactgt cggatatcaa
       61 cgctggatca gaccatcgaa aatcaacgcc gggaaatcga aagtgcaggt tttaaaatca
      121 aacctcagca aataatcgaa gaacacatta gcggctcagc agcaaccagt gagcgtcctg
      181 gttttaaccg gttgcttgct cgcctgaaat gtggtgatca attgattgtg acaaaactgg
      241 atcgccttgg ttgtaatgca atggatatca ggaaaacagt ggaacaactg accgaaacag
      301 gtatcagagt gcattgctta gcattggggg gcattgacct gaccagtcca acaggaaaaa
      361 tgatgatgca cgtaatttca gcagtcgctg aatttgaacg agacctttta cttgaacgca
      421 ctcattccgg gatagtaaga gcccgcggcg cagggaaacg ttttggtcga ccacctgtgt
      481 taaatgaaga acagaaacag gtggtattcg aacgaattaa gtcaggtgta agtataagtg
      541 ccattgcccg ggaattcaaa acctcgcggc aaaccatttt aagagccaaa gcaaaacttc
      601 agacacctga catataaaaa ataatctcgg tgtgagatgc tttacgtctt ccaagccccc
      661 ttccttgccg taaatggaaa gatacatcta attatagaat ttatatgttt taccctacgg
      721 cagtgctggc cattcaatat cctgtgcagt tga
//
LOCUS       DAZGOB010000462          746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZGOB010000462 DAZGOB010000000
VERSION     DAZGOB010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.465"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHLIF_005571"
     CDS             complement(<1..245)
                     /locus_tag="ACHLIF_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9609325.1"
                     /translation="MQHIHGESLNESVKSEQSPRVVLWEIDLKAQGGERYFFCNELNE
                     KGEPVTWQGRKYQAYPIDGSGFEMNGKGSSARPSLTVS"
     gene            complement(245..586)
                     /locus_tag="ACHLIF_005572"
     CDS             complement(245..586)
                     /locus_tag="ACHLIF_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609326.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNVRYS
                     DFSCTIEQVVN"
     gene            complement(579..>746)
                     /locus_tag="ACHLIF_005573"
     CDS             complement(579..>746)
                     /locus_tag="ACHLIF_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9609327.1"
                     /translation="SAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVML
                     SMRRDGGMLSG"
BASE COUNT          157 a          267 c          161 g          161 t
ORIGIN      
        1 gacaccgtca gcgacgggcg ggcactgctg cccttcccgt tcatctcaaa gccactgccg
       61 tcaatcgggt atgcctggta cttccgcccc tgccaggtca ccggctcccc tttttcattc
      121 agctcattac agaaaaaata ccgctcacca ccctgggcct tcaggtcaat ttcccagagc
      181 accacccgcg gtgactgctc tgatttaacc gactcgttca ggctttcccc gtgaatatgc
      241 tgcatcagtt caccacctgc tcaatcgtac agctgaaatc actgtaccgg acgttatccg
      301 tgacgctcca ctcccggcac accaccctca ccgtccggtt atgtttcggc ggtcgccaca
      361 aaaaggcacg gtaaccacca tgccaggata aaaattcatc cagccagcgc cgggttgact
      421 catccgtcac ccggaacacc gcctgaaacg tcttcagttg aggattcagt cctgtggggc
      481 gacgctgttc ataaccgtca ccaaaccgta ccctcgccac cgacggtttc tcactcacct
      541 gcatcccttc acgcgggacc agatgcagcg tttttatctc agccactcag cattcctccg
      601 tcacgtcgca tggacaacat caccgcctgc acccgctggt caatcagctg cacaagactg
      661 cctgccgcct ccggccctat ctggccatta gtcccgtcat tctgaatggc gatatggtag
      721 accggggaat acaccaggcc cgcact
//
LOCUS       DAZGOB010000463          745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGOB010000463 DAZGOB010000000
VERSION     DAZGOB010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.466"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..545)
                     /locus_tag="ACHLIF_005574"
                     /pseudo
     CDS             complement(1..545)
                     /locus_tag="ACHLIF_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          223 a          158 c          130 g          234 t
ORIGIN      
        1 tctgcaatat ctttctggca gcccatcgca tcagttgtga tgatttttcc tttaatatcc
       61 atcatgttaa gaagttcagg aatggctgta atctcattgg atttctcatc cgtcttgatc
      121 tgtccgagga ccagactatg cattgttgag aacgcactaa tgacatgaat cgctcccctg
      181 cgacgactct tgtcataaga gtgccggagc gtttttccat caattgcaat gacgtcttta
      241 tcatctgaag aatggcagtc acgcatccag ttaataaagc actcgtgaaa ttttgcagga
      301 ctgatacagg atacaactct ggcaatgata tcgtgaacag gaataccatt ttcaaaatca
      361 ccatatcact tcaaaacatc gggatgtgtt tccccaaaat cctctatatc ttcccaacct
      421 tctgcaccag aaataacggc acaaatagtc aacagtagaa tatccgataa tttatgttcc
      481 actttccagg tttgtctgta atcggggata atagaaatat gttccatcaa ttttttaagt
      541 tccattttgt tctccttaat taggtaagga gtatttgatc atgtataagc aataaaaaac
      601 agcttcaggt aataaggaat atctcaattt ttaaacataa aatgcgaatt atttagtgca
      661 aaaagtgaac ttttgatgat ctttccctgg ccgttgactc gattgccgta gctcgaacgc
      721 aagcactctt atcattcaat gccat
//
LOCUS       DAZGOB010000464          744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGOB010000464 DAZGOB010000000
VERSION     DAZGOB010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.467"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..663
                     /locus_tag="ACHLIF_005575"
     CDS             <1..663
                     /locus_tag="ACHLIF_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9609328.1"
                     /translation="NLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYI
                     TQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKE
                     YGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVT
                     KENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQG
                     SN"
BASE COUNT          262 a          117 c          169 g          196 t
ORIGIN      
        1 aacctgctga atgaagagaa cccgcatcag ctgcagcagc ttatccgcct gccggggcag
       61 cagtatgatg aggagtccgg cctgtattac aaccgccacc gctattatga cccgctgcag
      121 gggcggtata tcactcagga tccgattggg ctgaaggggg gatggaacct gtatacatat
      181 ccgctgagcc cggtgaatag catggatcca ttaggattat atgaatttaa atcaaaaaat
      241 atagatgata ttggaatatt tgcattggca atgtgtaatg gagaatcaat taacgagaat
      301 aaagaatatg gtggactaat atgtaagaag caaggtgaat atttccccat gaatccgata
      361 agttcaaatg ataatgatag tgtagacttg cgaaatataa aatgccctga aggttcagag
      421 agagtaggcg attatcacac tcacggtttt tactctgacg ataaaggaaa taaagtaaca
      481 aaagaaaatg atgtttatga tagtctaaat ttttcaagca aagatttaac gaattcttat
      541 atgaatggaa tgggaaaaaa agaatacagt agttacttgg gaacaccaaa taacacctat
      601 ctaaaatata atcccaaagc taaagggaat ggagttacaa ttatcaggca agggagtaat
      661 taaaatgaag gcaatacaat gttgttattt ccttgcttta gtctccagtc atttaatggc
      721 tggagttatg ggtgatgata atat
//
LOCUS       DAZGOB010000465          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGOB010000465 DAZGOB010000000
VERSION     DAZGOB010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.468"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..192)
                     /locus_tag="ACHLIF_005576"
     CDS             complement(<1..192)
                     /locus_tag="ACHLIF_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609329.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFS"
     gene            complement(173..731)
                     /locus_tag="ACHLIF_005577"
                     /pseudo
     CDS             complement(173..731)
                     /locus_tag="ACHLIF_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          218 a          163 c          110 g          240 t
ORIGIN      
        1 ggaaaaaaaa tctgggagcc atgtaaaaac tccatcattt tctggtattg ccatatagtt
       61 tatgtaatgt ttacttacaa gagattcatc tttgtataaa taaagataag taattacgca
      121 taaaacaaca atgattataa tagcaaaaat aaatattatc atctttgata gattacttga
      181 gatagccagc atcttgtaaa gcctttatcg tttttttatg ctctggatta atataatcac
      241 tacatctatc tgagcaatct gttgttgatg gacatgtcaa cccatggtca tttacagcca
      301 agtctttttt attatcttca atcatttcag aatgggatag ctttactttt ctgccgtaca
      361 taccgaacag atttaacccg taatctctaa tctctttttc ataacccagc cctttcgccg
      421 atcggctaac accagcgtca tttaatttag acactcgaca aaatgccata caatggaaaa
      481 attgatcagt cccacctaaa tttaatctct tcatatcttc ataagtatca gataatatat
      541 cccatgctct ttgatggttt agttgatctt ttcttcttat tagcgctaca tctgcgggac
      601 ttaaccctaa tggatcaata ccattcaccg gattcagcgg gtacgcatac agactccatc
      661 cccccctcca gccctatcgg gtcctgagtg atataccgcc cctgcaacgg atcgtagtac
      721 cggttccggt t
//
LOCUS       DAZGOB010000466          728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZGOB010000466 DAZGOB010000000
VERSION     DAZGOB010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.469"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..728
                     /locus_tag="ACHLIF_005578"
                     /pseudo
     CDS             1..728
                     /locus_tag="ACHLIF_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          191 a          201 c          198 g          138 t
ORIGIN      
        1 gacaaaaaca gtatcaacca gtaaaaaacc ccgtaaacag cattcgcctg aatttcgcag
       61 tgaagccctg aagcttgctg aacgcatcgg tgttactgcc gcagcccgtg aactcagcct
      121 gtatgaatca cagctctaca actggcgcag taaacagcaa aatcagcaga cgtcttctga
      181 acgtgaactg gagatgtcta ccgagattgc acgtctcaaa cgccagctgg cagaacggga
      241 tgaagagctg gctatcctcc aaaaggccgc gacatacttc gcgaagcgcc tgaaatgaag
      301 tatgtcttta ttgaaaaaca tcaggctgag ttcagcatca aagcaatgtg ccgcgtgctc
      361 cgggtggccc gcagcggctg gtatacgtgg tgtcagcggc ggacaaggat aagcacgcgt
      421 cagcagttcc gccaacactg cgacagcgtt gtcctcgcgg cttttacccg gtcaaaacag
      481 cgttacggtg ccccacgcct gacggatgaa ctgcgtgctc agggttaccc ctttaacgta
      541 aaaaccgtgg cggcaagcct gcgccgtcag ggactgaggg caaaggcctc ccggaagttc
      601 agcccggtca gctaccgcgc acacggcctg cctgtgtcag aaaatctgtt ggagcaggat
      661 ttttacgcca gtggcccgaa ccagaagtgg gcaggagaca tcacgtactt acgtacagat
      721 gaaggctg
//
LOCUS       DAZGOB010000467          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGOB010000467 DAZGOB010000000
VERSION     DAZGOB010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.470"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..125)
                     /locus_tag="ACHLIF_005579"
     CDS             complement(<1..125)
                     /locus_tag="ACHLIF_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9609330.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYT"
     gene            385..>712
                     /locus_tag="ACHLIF_005580"
     CDS             385..>712
                     /locus_tag="ACHLIF_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO9609331.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQ"
BASE COUNT          267 a          146 c          105 g          194 t
ORIGIN      
        1 gtataagggg ttatggccac cggactacca aacggctgcc agccctcttt cagtttatgt
       61 gtcagctttt ccgcaagatc tgatggcgac gccgccctga caacatcata gtgtttaaat
      121 gccatggttc tttccaccat ctgaaaaata attctttaaa atacctgaca tgtaatacag
      181 aaaaaacaca aaaccatacc ttaaataaaa acctgattat caagcagata tgcatggata
      241 aactacaaga cgagatataa accaccctgt atttaaataa acaataaaca acatcagaaa
      301 aataattctg ctctatggtt taattcaaaa atatcattta tacttttcag aacatcacca
      361 gcaaggcata aacaaggaaa gtaaatgaag tggattgtga ttgatacagt tatccagcca
      421 tcatgcggaa tatctttttc agtcatatgg agtaaaataa aattaataat ctggtatcaa
      481 tcggatgctt tcttacctcc tgaaagtata tttacactga ctcacacagg catcatgctc
      541 aataacaaag tgctacctgt aaccatttac aacgtagtac cattcaataa aacattctgg
      601 aatttaatca aaaacagcca ggaatgccct acaaatacag ataacgtatt gaatgaatgc
      661 tttaataacc gttgcactct gcaaatatgt ccttatggac taaaacaaca aa
//
LOCUS       DAZGOB010000468          707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZGOB010000468 DAZGOB010000000
VERSION     DAZGOB010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.471"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..529)
                     /locus_tag="ACHLIF_005581"
     CDS             complement(<1..529)
                     /locus_tag="ACHLIF_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO9609332.1"
                     /translation="MSFGQPCDEFPLSALPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGK"
BASE COUNT          197 a          163 c          146 g          201 t
ORIGIN      
        1 atttaccaca aagctgattc ttaattgccg cagtcgtggc atcattaaaa atgaatttat
       61 acctcacagg ttcttccgca ctgtttttct gaagtacttc cagttgtctg agggtttctg
      121 actcatccgc gcctttgcgg agttctttat tcagttttga caacaatgct tttttctgag
      181 cctttaaaag ctcttccttg tttttccaga cagccagttc acttttgtat ctggaatata
      241 actgcatctc ctgctggtac aggggcttca ttaataattt gtccactgcg gtttttcgtt
      301 ctccggactc ggcaagcgtc aggaaaaaaa gagagaccgg cccccgcagt gtattcagtc
      361 tgcagacatc aatcagattc tggcagacaa gtgaaacagc gcccagggca gacgctgcca
      421 caagcccaag gggagcctgc gtaatttgct gagcttcaat aacggcatca cgaatgagtg
      481 gaggcagcgc actcagggga aactcatcac aaggctgacc gaatgacata gtaacctcac
      541 tacacacaga tataaaagca ctgcaattct ggctattaat agcaaaaata gcagtcaaat
      601 caatatgagg taaaaatttt tgttgattta atggttaaat aataaccaca gtgtgttttg
      661 ctgctgaaga aagacaaaat gaatctgtgt gtcccctcct tcccttc
//
LOCUS       DAZGOB010000469          706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZGOB010000469 DAZGOB010000000
VERSION     DAZGOB010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.472"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>706)
                     /gene="nleL"
                     /locus_tag="ACHLIF_005582"
     CDS             complement(<1..>706)
                     /gene="nleL"
                     /locus_tag="ACHLIF_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO9609333.1"
                     /translation="MNTSDDKYNHDKVRAAEELIKKISLDELAAFRPYVKMSLADSFS
                     IHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMENGSLLQAGMYFERHPGAMVS
                     YNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDD
                     SDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGYSLS
                     NLFSESFPIFSIPYHKA"
BASE COUNT          247 a          149 c          101 g          209 t
ORIGIN      
        1 agctttatga taaggaatac tgaaaattgg aaatgattca gaaaaaagat tgctcaatga
       61 atacccaaat gtattttgaa cttctccatc cttatataaa aaaaaggaca tccagttagt
      121 tgatgtatta ggagataaca tatcaaccat atgactcaaa gaacacatca ttgccatacc
      181 atcatcctgt gatgataaca aaacccagtt ataagccaaa tcggaatcat catcccagtc
      241 aggcttccca gagccatcgc acaatccaaa atcactctgc tgtgtgacag gataaacttt
      301 ttcatttttt aaatatactt catagagttc cctaaatcgt tcctgcattc catcacgccg
      361 tgaaccattc attacaattt gtataaagga actattataa gataccatcg cacctggatg
      421 tcgctcaaaa tacatccctg cctgcaataa actaccattc tccatataca tcattattga
      481 attattaaac caagacatta tagtatcaaa aaagtcatca catataggct cgagccattg
      541 ctgtatattt gcgttgttca aataaggatg aatactaaat gaatcagcca aagacatctt
      601 aacatagggc cggaacgccg ctaattcgtc aagagatatt tttttgataa gttcttcagc
      661 ggcccttact ttatcatggt tatacttatc atcagatgta ttcata
//
LOCUS       DAZGOB010000470          706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGOB010000470 DAZGOB010000000
VERSION     DAZGOB010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.473"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..698
                     /locus_tag="ACHLIF_005583"
     CDS             <1..698
                     /locus_tag="ACHLIF_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HFO9609334.1"
                     /translation="PAVLFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRI
                     TEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKT
                     KPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENAL
                     RMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRV
                     GELLPWRVALPTE"
BASE COUNT          167 a          196 c          220 g          123 t
ORIGIN      
        1 cgccggcggt gttgttcgct tacagcccgg acagaaaagg catccatccg cagacccatc
       61 ttgcggggtt cagtggtgta ctgcaggcgg atgcatacgc cgggttcaac gagctgtacc
      121 gggatggccg gataacggaa gccgcctgtt gggctcacgc ccgccgtaaa atccacgatg
      181 tgcacgttcg caccccgtca gccctgacgg aggaagcgct gaaacggatc ggcgaactgt
      241 acgccatcga ggcagagata aggggaatga cggcggagca gcgccttgcc gaacgtcagt
      301 tgaaaacgaa accgctgctg aaatccctgg aaagctggct gcgtgaaaag atgaaaaccc
      361 tgtcgcgaca ctcagaactg gcgaaagcgt tcgcatacgc cctgaaccag tggccggcgc
      421 tgacgtacta tgcagatgat ggctgggctg aggcggacaa taacatcgct gaaaatgcgt
      481 tgcggatggt cagtctgggc cgcaaaaact acctgttctt cggttcggat catggaggag
      541 agcggggagc gctgctgtac agcctgatcg ggacgtgcaa actgaacgga gtggagccag
      601 aaagctacct ccgctatgtc cttgacgtca tagccgactg gccgataaac cgggtcggcg
      661 aactgctccc ctggcgcgta gcactgccga ctgaataaca catccc
//
LOCUS       DAZGOB010000471          699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGOB010000471 DAZGOB010000000
VERSION     DAZGOB010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.474"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(312..>699)
                     /locus_tag="ACHLIF_005584"
     CDS             complement(312..>699)
                     /locus_tag="ACHLIF_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9609335.1"
                     /translation="PLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAVRR
                     AEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFALDM
                     AATLVVPFTGDGRDAAEILRTLPPLK"
BASE COUNT          144 a          199 c          182 g          174 t
ORIGIN      
        1 cgtcatagca gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact
       61 gccagctgaa taacacatcc ccgtcaatac ggccctcgct gtacgcttac gtttatattc
      121 agtttcctgc agtgctgcct gtggctgagc tgccatctgc ctgtccctta cgtgagtcac
      181 cccgtaacct gatgctgagg cattgctccc ttcataaaac atggtttact cactacagct
      241 tatctacctg ctccagctta tgttatgtct gttctgctga ccactgctta tataaggaag
      301 agcgtatgct gtcacttcag gggaggcagt gtacgcagga tctctgcagc atcgcgcccg
      361 tcacctgtga aaggcactac cagcgtggca gccatatcca gcgcaaaaac tctggtataa
      421 acctccatcg aacgtggatc cctgtgacca gccagtgcct ggatgacttt ccggggctgg
      481 cggtgataga gcatgtgcat gatatagctg tgccggaagg tgtgtggtgt gaccggaatc
      541 gaaaagtgta ccccgtcagc ttcggcccgt ctgacagcct gcttcagcca gttgcgcatg
      601 gtttcgtcag tcacggccca taatggttca cgacgacggg gccgggtggt gatcatccag
      661 ctttccatct gcctgacata gcttatatct gtcagcgga
//
LOCUS       DAZGOB010000472          696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGOB010000472 DAZGOB010000000
VERSION     DAZGOB010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.475"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..425)
                     /locus_tag="ACHLIF_005585"
     CDS             complement(<1..425)
                     /locus_tag="ACHLIF_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO9609336.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCT"
     gene            complement(538..>696)
                     /locus_tag="ACHLIF_005586"
     CDS             complement(538..>696)
                     /locus_tag="ACHLIF_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9609337.1"
                     /translation="LESDENGIIPEQARVITQVVILDADKKQIQCVVRPLQILRADGT
                     WENIGGMK"
BASE COUNT          202 a          192 c          120 g          182 t
ORIGIN      
        1 gtacatacat ctgaaccatc tgaattcttc acaaaaatac ctttttcagg ctgctccagt
       61 gtaattggac attgaatagc ctcaagcggg cactgaagtc tctctggaca aacagaaaac
      121 ttacattgag atattttgtt ctgaatactc tcttgcgatt gtgcttcacc tgagtccgaa
      181 agcatactta gcattctctc accaagggct cgaggaccac tattaaatcc caaaagcaat
      241 aactcagaaa gcaatccgcc attaccgcca ctggatgaaa agcgcccatt agtagaatca
      301 tacacaatac tgacagtttc accaccgact gtaatatctc cgcgcccatt ggctacaatt
      361 cgggcacgaa caacctccac cccaagatta aatgaatgtg atctaatatc tgaggttaat
      421 ggcataacaa agctccctat tttaatttga actccagact taaatagctg taacaaacat
      481 cccccataca tgacaacaaa aaccggagcc ggactccggt ttttgtgaag ctgtcggcta
      541 tttcattccg ccaatatttt cccacgtccc gtcagcacgc agaatttgca gcggtcttac
      601 cacgcactgt atctgctttt tatccgcatc cagtatcacc acctgcgtga ttaccctggc
      661 ctgctccggg ataatgccat tctcatcgga ctccag
//
LOCUS       DAZGOB010000473          695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGOB010000473 DAZGOB010000000
VERSION     DAZGOB010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.476"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..653
                     /gene="espW"
                     /locus_tag="ACHLIF_005587"
     CDS             <1..653
                     /gene="espW"
                     /locus_tag="ACHLIF_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO9609338.1"
                     /translation="IEDFHKKYNETRLKANTNIQQKQIADEVKSLTYLIPSEKKEMWI
                     YKNNGKDNAKPNLGERDVRMFENISPDDTDKITGRKFSELGEYLYSGNVIKLSQLSIR
                     YLPNINSISLIETKQSLLLHRLYSDEVLQRNGTLIPTPLHEEKSIPADNIKTMLNNIP
                     TYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSPRNYGLHHNIKTWDPLVRN"
BASE COUNT          286 a          121 c          113 g          175 t
ORIGIN      
        1 gcattgaaga ttttcataaa aagtataatg aaactaggct aaaggccaat accaatatcc
       61 aacaaaaaca aattgcagat gaagtaaaat cacttacata tttgattcct tcagaaaaaa
      121 aagagatgtg gatatataaa aacaatggaa aagataatgc aaaaccaaac ttaggagagc
      181 gagacgtaag aatgttcgaa aatattagtc ctgatgatac agataagata acaggaagga
      241 aattttcaga gttaggtgag tatctttatt caggaaatgt aataaaactc agtcagttat
      301 caattcgtta cttacccaat atcaactcaa tctcattaat agagacaaaa cagagtttgt
      361 tgctacatcg attatattca gatgaagtac ttcagagaaa tggaacactt atcccgacac
      421 cactacatga agaaaaatca attccagctg acaatataaa aacaatgctc aacaacatac
      481 cgacttacaa aatgttaccg ccattcacag aaacacaagg taattgttct tctggcgcag
      541 ccacgttttt acgcaaatca ggcgccgaag aaaaagatat tcttgcatgt agcccccgaa
      601 attatgggct gcatcataac ataaaaacat gggacccctt ggttagaaat taaggatcca
      661 aatattatat atttcatata aagcaaggaa aatat
//
LOCUS       DAZGOB010000474          694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGOB010000474 DAZGOB010000000
VERSION     DAZGOB010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.477"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            296..511
                     /gene="essD"
                     /locus_tag="ACHLIF_005588"
     CDS             296..511
                     /gene="essD"
                     /locus_tag="ACHLIF_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO9609339.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            511..>694
                     /gene="rrrD"
                     /locus_tag="ACHLIF_005589"
     CDS             511..>694
                     /gene="rrrD"
                     /locus_tag="ACHLIF_005589"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO9609340.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIM"
BASE COUNT          170 a          138 c          170 g          216 t
ORIGIN      
        1 tttgttgaga tttgtttatg tatttgtagt ggtgttttca atactcggta gcattctcgc
       61 aaatatcatt tagtggttta cgtacgtaaa aaattggtta tgctgttaag agtggttact
      121 tcgtcacaca gcttaaaccc gccgtcgagc gggtttttcc attttttgag tctcgatatt
      181 agctgataac ccaatacctg agttattcac tgactccgag tctgttacgt ttctgctttt
      241 ttgcgatacg ttgtattccc tcaatttaca cccgctttgt ctgcgaggtg gggttatgaa
      301 atccatggat aagttaacaa cgggtgtcgc ctatggcacc tcagcaggta gtgccggtta
      361 ctggttttta cagctgctcg ataaagtcac gccctcacag tgggcagcaa taggtgtgct
      421 gggtagcctg gtatttggcc tgctgacgta cctgacaaac ctttatttca agattaaaga
      481 agataagcgc aaggctgcga gaggtgaata atgcctccat cattacgaaa agccgttgct
      541 gctgctattg gtggcggagc aattgctata gcatcagtgt taatcactgg cccaagtggt
      601 aacgatggtc tggaaggtgt cagctacata ccatacaaag atattgttgg tgtatggact
      661 gtatgtcacg ggcatacagg aaaagacatc atgc
//
LOCUS       DAZGOB010000475          689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGOB010000475 DAZGOB010000000
VERSION     DAZGOB010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.478"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(103..477)
                     /locus_tag="ACHLIF_005590"
                     /pseudo
     CDS             complement(103..477)
                     /locus_tag="ACHLIF_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515619.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT          185 a          133 c          159 g          212 t
ORIGIN      
        1 ttttatttgt taatattaat tttagcaggg ctaactttcc ggtaagaatt aattactggt
       61 catatgctcc ttggttaata aaattctatg ccttacttgg tgctagctgg ctttaatttc
      121 tccatgtgga actacaaccc agtcgatatg gttttgcgtg tagattttag ttgatttcgc
      181 atcgctgtgt gccattcgtc cttgtggatc gatgccctgc tgatcaaaaa gatgtgctgc
      241 taaggcgcga atttcgtgaa aggttggcct ttcatccatc ggccgtttgt catataaacc
      301 cagcttgtca cgcgtagctg aaaacgaccg actcaaatag tctggtgcaa cttgagtggg
      361 atgagaaact tctttgctgc gtttaacctg ccgttccggg atctggtgaa cgataaatgg
      421 gctggccacg ttattgcgac tttcatcaat gatccgcttc aattcatcac caatcggtga
      481 ccgctacatg acgggaacgc ttacaatcta ttaatgtcta ctaagtgcta ggagcggaca
      541 ttggttaatg gggatatgtc attcgcctgg ggcagatcaa gaaagtaaaa atcccagctg
      601 tcgataattg gaatccatga taaggtttac gggtaactga ttgataaata aattgaatgg
      661 aatatggaaa aatgacaaag tcaatagcg
//
LOCUS       DAZGOB010000476          683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGOB010000476 DAZGOB010000000
VERSION     DAZGOB010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.479"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>683
                     /locus_tag="ACHLIF_005591"
     CDS             <1..>683
                     /locus_tag="ACHLIF_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9609341.1"
                     /translation="QRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKI
                     SGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMA
                     DDGQLTADKVVPALISQLGVLRDEYAAMPETVSGSITKVENAFMAWVGGVNEASGVTK
                     TLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAE
                     AQFRGTQIAT"
BASE COUNT          139 a          155 c          238 g          151 t
ORIGIN      
        1 cagcggacag gtaccgcttt ttcggataat gcggccctgt ttgcccgttc ggctgcctcg
       61 atgcgtgaat atggttacag tgctgatgat gtgctgaagg tgacggaggc catttccacg
      121 gggctgaaaa tctccggtgc cagtacggct gaggcgggtt cggtgatcac ccagttcagc
      181 caggcgctgg cacagggtgt gttgcgcggt gaggaattta attcggtcaa tgaaagtggt
      241 gaccggatca ttcgtgcact ggctgcaggc atgggcgtgg cccgtaaaga tctgaaggcg
      301 atggcggacg atggtcaact gacggcggat aaagtcgttc ctgcgttaat cagccagctg
      361 ggggtattgc gtgatgaata tgccgccatg ccagaaacgg tttccggtag tatcacgaag
      421 gtggaaaacg cctttatggc ctgggtgggc ggagtgaatg aggccagcgg ggtgacaaaa
      481 acgctctccg gcgtgctgaa cggtgttgcc ggacagattg ataatgtggc aacagctgtg
      541 ggtgcgctgg ttgccgtcgg ggttgcccgg tactttggca atatggcctc cggagcgatg
      601 tctgccacgg caggacttgt gacggctgca cgtaatgaag ttgcactggc ggaagcacag
      661 ttcaggggaa cgcagattgc cac
//
LOCUS       DAZGOB010000477          676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGOB010000477 DAZGOB010000000
VERSION     DAZGOB010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.480"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..369
                     /locus_tag="ACHLIF_005592"
     CDS             <1..369
                     /locus_tag="ACHLIF_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9609342.1"
                     /translation="RFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLAT
                     PTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDKPTTDIRKDRCSKCMRGC
                     EMRGMVANFGGFLSINKLSQ"
     gene            375..>676
                     /locus_tag="ACHLIF_005593"
     CDS             375..>676
                     /locus_tag="ACHLIF_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9609343.1"
                     /translation="MTQTESAILAHARRCVPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEI"
BASE COUNT          132 a          159 c          239 g          146 t
ORIGIN      
        1 cgttttctgg atgcggtgaa tttcgttgcg ggcaatccgg aggcggaccc ggagcaggag
       61 ctgagtgacc gctgggtggt ggagcagatg tcgcagctga cagccatgac ggcctcgttt
      121 gtgctggcta caccgaccga gacggatggg gcgctgtttc ccggtcgcat catgctggcg
      181 aacacctgta tgtgggatta ccggggagat gaatgcgggt ataacggtcc tgcggtggcg
      241 gatgagttcg ataaacccac caccgatatc cgtaaggaca gatgcagcaa gtgcatgcgc
      301 gggtgtgaga tgcgcggcat ggtggctaat tttggcggtt tcctttccat taacaaactt
      361 tcgcagtaaa tccaatgaca cagacagaat cagcgattct ggcgcatgcc cggcggtgtg
      421 tgcctgcgga gtcgtgcggc ttcgtggtga gaacgccgga gggggagcgg tatatccctt
      481 gtgtgaatat ctctgcagag ccggaggcgt attttcgtat tgcaccggaa gactggctgc
      541 gggcagagat gcagggggag attgtggcac tggtccacag tcatcccggt gggctgccct
      601 ggctgagcga ggctgaccgg cggctgcaga taaaaagtgc actgtcctgg tggctggtct
      661 gccgggggga aattca
//
LOCUS       DAZGOB010000478          648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGOB010000478 DAZGOB010000000
VERSION     DAZGOB010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.481"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..211)
                     /locus_tag="ACHLIF_005594"
     CDS             complement(<1..211)
                     /locus_tag="ACHLIF_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609344.1"
                     /translation="MSTGLRFTLEVDGLPPNAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQR"
BASE COUNT          210 a          134 c          146 g          158 t
ORIGIN      
        1 ggcgctgtac ttcatcgccc tgccatatcg tcaggtaggc cattttgtcc agcacctgcg
       61 caaattcaag ggagagaaac tgctggctga ccagggagag atcgagggaa aagagcgaag
      121 agagtgactg gttcagatga aaggaaacca ccgcaaaagc attcggcggc aggccgtcca
      181 cttccagtgt gaaacgtaat ccggttgaca tagcaacctc cgtgaaaatg aataataatc
      241 cgcacatatt gcatgtacaa catgtcgggc ggatgagata agcgagtcga catctggcgt
      301 taccggggaa tatcagaaaa atgtcatggt tatgtgatga tttagtctgg gattttactt
      361 cagagaaacg ggtgtctgtt tagtcaacaa aataatcaca aacgcaacgg atgggttctc
      421 gacgttattc acgacaggct cgtacattac agcgagtaat aaaaaacctg atatgcaaag
      481 tcaaaccaat attcataatg tctttttatt cacaacaata aataattcaa taacaatcat
      541 tgaaatgaat gatggtttgt ataaggaata tacgctaccg ccccaccctg ataacatgat
      601 ctgcacattg ccatcttaac gtcgttaaac actagcttaa tagtggat
//
LOCUS       DAZGOB010000479          647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGOB010000479 DAZGOB010000000
VERSION     DAZGOB010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.482"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            10..468
                     /locus_tag="ACHLIF_005595"
     CDS             10..468
                     /locus_tag="ACHLIF_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609345.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            568..647
                     /locus_tag="ACHLIF_005596"
                     /pseudo
     CDS             568..647
                     /locus_tag="ACHLIF_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          210 a          115 c          111 g          211 t
ORIGIN      
        1 agttactata tgaaaatata ctggacaaga aaacgtattc ctgaactttc agcacttcca
       61 ccttcattaa gaaagaaaaa ttttactgac gcatataatg cagcatcttc tcatatcgaa
      121 tactggatag gtgctggcgt ctcatttatc tcgatgatga ttttatccag agtatatgac
      181 tttcttttac ccgcccaaga tactttcccg ggtgatatca tcagaagttt atgtgttgtg
      241 tgtccatcca tattaatctg gtttcagttt tcggtatatg tcatgaggaa atattacaga
      301 catattctgg tgcgaggaaa agaaaccgaa accatttcag aaaggttgat acgtgaggct
      361 gatacacgag aatatgaact atggcgtccg gtccgtcgct ttttctccat tgttttcctc
      421 attgttctat taggttgcat tcattcactg ataacaacta taaaataaaa gttatggctg
      481 ttctgataca ggaaaataaa cgttcattct atggttacct cttcctgtta tatagaagaa
      541 ttttaaacga caagtaaggg aatataaatg aaaataacaa aaaataaaaa tgcaactata
      601 aaaaacagtc atcagtcggc tgcttttcaa tgtttacctt atcattt
//
LOCUS       DAZGOB010000480          647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGOB010000480 DAZGOB010000000
VERSION     DAZGOB010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.483"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            120..338
                     /locus_tag="ACHLIF_005597"
     CDS             120..338
                     /locus_tag="ACHLIF_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609346.1"
                     /translation="MFKYAEMLMAEEDDKVCELIFKQQDNLILKINVRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLIVCTLGVNS"
BASE COUNT          206 a           87 c          120 g          234 t
ORIGIN      
        1 tgaatactgc caaatattat tgcaaaatat tctctaaatt atgaattaaa tatccataag
       61 cattattgcc tgggaactgg acatataatt actcttctgt gctaaaggaa cgttctgaaa
      121 tgtttaagta tgcagagatg ttgatggcgg aagaagatga taaagtatgt gaacttattt
      181 ttaaacaaca agataatttg attttaaaaa taaatgttag atttcccaga ataattataa
      241 agaaataccg aacattacaa ggtaaagtcg tatggctatg gatattgcca tttgttattg
      301 aatggttaat tgtgtgtact ttgggtgtaa acagttagct tggtaaaagt aaatatcctg
      361 attgttgttt gttaaggagt gagatgcctg tgcgtctggt gtctattata ttcccaatat
      421 tattggcgct attcttgctg gcgtaggcat cgccatacta tgaatattgt gtttgtcgtg
      481 attcaatttt tatttataac ctctacccgg agcaaattca ggtaggtcat gcataaaaat
      541 atctttctat actacgaaaa ttatttttgt gaaagtattg acaacgctaa ctatttttaa
      601 ttatatattc atgatgctct aggcatcaca ttttctccat ggggtat
//
LOCUS       DAZGOB010000481          645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGOB010000481 DAZGOB010000000
VERSION     DAZGOB010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.484"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>645
                     /locus_tag="ACHLIF_005598"
     CDS             <1..>645
                     /locus_tag="ACHLIF_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO9609347.1"
                     /translation="DKNSRNGLKLLQKQADLLKIIMEDTSVTENIFKNIEMAIADIKK
                     EYYSHTVDIEKNIHAIWVAGSPPESISDYIKTFLKTYKEFTYYLWVDEKAFGAAKFTS
                     VLKQIAFDLACRTIQQNTPQKNIDFINLYNEIRKKYNNNPSGQQEYLNKLRELYATYQ
                     KISTPLKHMFNSFFLENMIKLQDNFFNYCIVKGVTEINDELRINYLKNVIKLSDD"
BASE COUNT          254 a          107 c           99 g          185 t
ORIGIN      
        1 gataaaaaca gtcgtaatgg tctaaaactt ctacagaagc aagctgattt actaaaaata
       61 atcatggagg atacctctgt tacagaaaat atctttaaaa acatagagat ggctatagca
      121 gatataaaaa aggagtacta ttcgcataca gttgatattg agaaaaatat tcatgccata
      181 tgggttgcgg gttccccacc tgaaagcatc tcggactata ttaagacttt tctcaaaacc
      241 tacaaagagt ttacttacta tctttgggtt gatgaaaaag catttggagc cgcgaaattt
      301 accagtgtct taaaacaaat tgcatttgat ttagcatgca gaactataca gcagaatact
      361 ccacaaaaaa atattgattt tattaatcta tataatgaaa taaggaagaa atacaacaac
      421 aacccatcgg gacaacaaga gtacttaaac aaactcagag agctttatgc tacttatcaa
      481 aaaatatcta cccctctgaa acacatgttt aattcatttt ttctagaaaa catgattaaa
      541 cttcaggata atttcttcaa ctattgcatt gtcaaaggtg ttacagagat taatgatgag
      601 ttacgaataa actaccttaa aaatgtaata aaactgtcag acgat
//
LOCUS       DAZGOB010000482          643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGOB010000482 DAZGOB010000000
VERSION     DAZGOB010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.485"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            117..305
                     /gene="dicB"
                     /locus_tag="ACHLIF_005599"
     CDS             117..305
                     /gene="dicB"
                     /locus_tag="ACHLIF_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO9609348.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            302..490
                     /locus_tag="ACHLIF_005600"
     CDS             302..490
                     /locus_tag="ACHLIF_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO9609349.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
BASE COUNT          202 a          130 c          139 g          172 t
ORIGIN      
        1 aaccaaaaac agtgttatgg gtggattctc tgtatccggc gttaattgtt aactggttaa
       61 cgtcacctgg aggcaccagg caccgcatca caaaattcat tgttgaggac gcgataatgg
      121 aaacgttatt accaaacgtt aatacgtctg aaggttgttt tgatattggt gttctgctca
      181 gtaaccggga gtttactgaa gatgccatta atatgaggaa atatgagcct tatctgctca
      241 atgataattc catactttcc cgaattgctc ttcttgaact tggtattttc ggagaacgtc
      301 aataacttca gcatttgcac tgatgatgac ggtttttctt ataacgggtg aatcacagaa
      361 tgtgattacc ggaatttatg ccagtaaaga atcctgcctc caggcaagag acgagcaaaa
      421 aatttctggt gaatgcctcc cggtaaaaaa agtatcgctg tacctgaata acgaaacacc
      481 ggctggataa ccctccagcc atattaacac cataccaacg gattaaaaat gccagcaatg
      541 gcagggattc gttcaccctg aaatctgtaa tgaggtaaaa caaaatgagt aaagtcttta
      601 tttgcgccgc cattccggac gaacaggcaa taaaggaaga agg
//
LOCUS       DAZGOB010000483          638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGOB010000483 DAZGOB010000000
VERSION     DAZGOB010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.486"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..76)
                     /locus_tag="ACHLIF_005601"
     CDS             complement(<1..76)
                     /locus_tag="ACHLIF_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609350.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGF"
     gene            complement(60..>638)
                     /locus_tag="ACHLIF_005602"
     CDS             complement(60..>638)
                     /locus_tag="ACHLIF_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9609351.1"
                     /translation="NRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLD
                     AIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLM
                     CRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACI
                     EKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          183 a          160 c          108 g          187 t
ORIGIN      
        1 caaacccaag gtaaacaaca aataaacaaa gggtaattaa aaaaatgaca agtttgcctc
       61 taataatttg aagcatctga actcccttgc tttgctttta ttggattatc tgcttttata
      121 ccatctaacc cgaagagttg cccagtgtta tatttttcaa tacatgcatc gctacatttg
      181 gcagaaaaat cgccacaagc aataccgaca gtgttatttt taagatccat gattctttct
      241 ttagctggct gaccttgtct atcccctgcc gcctcatgat ttttacctat cacgtcagct
      301 attgttgatc caaatgtctt agtcattcga cacatcaagt aacaatgtct cattgcatcg
      361 gcggccccat tatgagctcc gccaacaata gcatccatat cataaccgaa ctggtaagca
      421 ctgttagtaa tgctgtttgc agcaatcaat ccaggtacac cagatacggc ataaattagt
      481 ccacctgatg tcatattctc aatcgcatct aaccccattg ggtctatcac ttgtatggga
      541 ttcaacggat actgatacat attccatcca cctctcaacc caatcgggtc cggggtgata
      601 taccgcccct gcaacggatc gtagtaccgg tgacggtt
//
LOCUS       DAZGOB010000484          635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZGOB010000484 DAZGOB010000000
VERSION     DAZGOB010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.487"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..211)
                     /locus_tag="ACHLIF_005603"
     CDS             complement(<1..211)
                     /locus_tag="ACHLIF_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO9609352.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDELQR"
BASE COUNT          214 a          125 c          134 g          162 t
ORIGIN      
        1 ggcgctgtaa ttcatcgccc tgccatatcg tcaggtaggc cattttgtcc agcacctgcg
       61 caaattcaag ggagagaaac tgctggctga ccagggagag atcgagggaa aagagcgaag
      121 agagtgactg gttcagatga aaggaaacca ccgcaaaagc gtccggcggc aggccgtcca
      181 cttccagtgt gaaacgtaat ccggttgaca tagcaacctc cggtaaaatg aataatgatc
      241 cgtaccgtac aactgcggat aatgtgataa gcgggacgac ttcagacgtt attagggaag
      301 gaacagaaaa tgctatggtt atgtgagaat tttgtttaaa tattacttta cagaaacggg
      361 tgtctgttca gtcaaaaaaa taatcacaag ctcaacctct tgtttcttgg cgttattcag
      421 gacaggtacg catatcacaa cgagtaacaa aaacgcaaag ttaaaccaat attcataata
      481 tatttttatt cacagcaata aataatccaa ttaacattat taaaatgaat gattgcataa
      541 gaaatatatg ctaccgctcc accctgataa catgatctgc acattgcaca tcttaacgtt
      601 gttaaatact ggctcaatag tggatatttt aaatt
//
LOCUS       DAZGOB010000485          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGOB010000485 DAZGOB010000000
VERSION     DAZGOB010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.488"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..465
                     /locus_tag="ACHLIF_005604"
     CDS             61..465
                     /locus_tag="ACHLIF_005604"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9609353.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            462..>628
                     /gene="tnpB"
                     /locus_tag="ACHLIF_005605"
     CDS             462..>628
                     /gene="tnpB"
                     /locus_tag="ACHLIF_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9609354.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKL"
BASE COUNT          175 a          165 c          150 g          138 t
ORIGIN      
        1 tctggcagat cctgaaattc ctgagagaat agtggacacc aaatatggtg gacgctatcc
       61 atgaaatcat taaccgcagt gcgtaaaaaa agccctaatt atcccgttga gttcaaaatc
      121 aaaatggttg aactctcgca tcgaccagag atctccgtag cgcaactcgc tcgtgagcat
      181 gggatcaacg ataatttgct gttcaagtgg cgccagtact ggcgcgaagg aaaactacgt
      241 cctccttcaa caacagaaaa caacgtgcct gagctgctcc cgataacact tgatgccgaa
      301 gatgttgtcc ctacaacctc cccccggtca caacctgtag ctgctgcgac acctgaatca
      361 ctcaatatca gctgtgaagt gacgttccgg cacggatcac tccgtctgaa tggtgccatc
      421 agcgaaaata tcctgaacct gctgatacgg gagctcaaac gttgatccca ttaccatcag
      481 ggacaaagat ctggctggtc gctggcatca ccgatatgag aaacggcttc aacggcctgg
      541 cggcaaaggt gcagacgacg ctgaaagacg atccgatgtc aggtcacgtt tttatcttcc
      601 gtgggcgtaa tggcagtcag gtaaagct
//
LOCUS       DAZGOB010000486          627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZGOB010000486 DAZGOB010000000
VERSION     DAZGOB010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.489"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(26..355)
                     /locus_tag="ACHLIF_005606"
                     /pseudo
     CDS             complement(26..355)
                     /locus_tag="ACHLIF_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
     gene            complement(375..>627)
                     /locus_tag="ACHLIF_005607"
     CDS             complement(375..>627)
                     /locus_tag="ACHLIF_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFO9609355.1"
                     /translation="EAFFIDPERASNRIFIERVNDYIERAGYNMIPVGECSNGHITLL
                     YGENNVIYGGLDDYLWKYGDNLSSSIDILINGKEAVEIP"
BASE COUNT          146 a          172 c          139 g          170 t
ORIGIN      
        1 accggcgtta catgatcttt ccctggctcc acgtctggtc gttatcggac cagcggatga
       61 tccggtccgc agcgtcgtac tcataaaaca ggtggtagtc cagccgcgca tccgcttcgg
      121 tcagccgccc ccgcgcatcc tgttcgtagg tcgccagcgt ctgtatgccg ttatccgtgc
      181 ggtgaatggc tttcaggtag ccgctggcat ggtacgtcag tttaagccgg atcccgtcgg
      241 tgtgactgac acacgtgatc cggtactggc tgtcgcgctc aaacgtgatg cggttgctgg
      301 cggtgtcact catcccgccc agcatataca tccggtcgtc ggggttgtgt gccaggtgtc
      361 atatcaagtt ggccttacgg aatttcaact gcctccttgc cattaatcaa tatatcaatt
      421 gatgaagata aattatcccc atacttccaa agataatcat ccaagccgcc atatatcaca
      481 ttattttccc catataataa tgtgatatga ccattactgc actcgccaac aggaatcata
      541 ttgtaaccag ctctctcaat ataatcattg actctttcta taaaaatgcg attagaggcc
      601 ctttctggat ctataaaaaa agcctca
//
LOCUS       DAZGOB010000487          626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZGOB010000487 DAZGOB010000000
VERSION     DAZGOB010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.490"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..612
                     /locus_tag="ACHLIF_005608"
     CDS             <1..612
                     /locus_tag="ACHLIF_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HFO9609356.1"
                     /translation="NQLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLRGRYI
                     TQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKA
                     LTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDG
                     KKTDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
BASE COUNT          200 a          131 c          151 g          144 t
ORIGIN      
        1 aaccagctga atgaagagaa cccgcaccag ctgcagcagc tcatccgcct gccggggcag
       61 cagtatgatg aggagtccgg cctgtattat aaccgccacc gctattatga cccgctgcgg
      121 gggaggtata tcactcagga tccgattggg ctgaaggggg gatggaattt ttatcagtat
      181 ccgttgaatc cagttacgaa tacagatcct ctggggttag aagtttttcc tagaccattc
      241 cccttgccaa ttccatggcc caaaagccct gcacagcagc aagcagatga taatgctgca
      301 aaagcattga caaaatggtg gaacgataca gcatcacaaa gaatatttga ctctctaata
      361 ttgaataatc cgggactagc attagatata acaatgatag cttctcgtgg aaatgttgca
      421 gacacaggga taactgatcg tgtcaatgac ataataaatg acagattctg gagtgatggg
      481 aaaaaaaccg acagatgtga cgtacttcag gaactaattg attgtggtga tattagtgct
      541 aaagatgcaa aaagcacaca gaaagcctgg aattgtcgtc actccagaca gtcaaacgat
      601 aaaaaaagat agcccttgtg gaggtt
//
LOCUS       DAZGOB010000488          626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZGOB010000488 DAZGOB010000000
VERSION     DAZGOB010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.491"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(7..141)
                     /locus_tag="ACHLIF_005609"
     CDS             complement(7..141)
                     /locus_tag="ACHLIF_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609357.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            complement(217..528)
                     /locus_tag="ACHLIF_005610"
     CDS             complement(217..528)
                     /locus_tag="ACHLIF_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HFO9609358.1"
                     /translation="MKLRVWHIPQVPMKPFIAEVASVEEGVRLMGALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEVGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
BASE COUNT          158 a          154 c          138 g          176 t
ORIGIN      
        1 taatggctat ttcattgcgc ataacgcctc cagttgtttc tttgctgctc taattaattg
       61 tttaactcgg cgtgataatt cagattcgtg cgggtagaaa gcggacatga cgccgctacc
      121 cgcgagctga aagtgcatca tgggtaactc cttatatttg attgcataac gaaaacgcct
      181 cgagtgaagc gttattggta tgcggtaaag ccgcgcttag gcggctgatg tttcttcttt
      241 caggctttcg agatatttac gtgggtcgtc gtaacattgg cattcgctgt accaatccac
      301 ccagcgatca gtaagcccca cctctgataa atcttcatcg gtaaggctct catcccacat
      361 ctcaaggccg ttagcattgc agtaatcagg cttgatgttg ttgtcatact gaaaggcgtc
      421 ataatcagcc agtgcgccca tcaggcgaac accctcttca acacttgcca cttctgcaat
      481 gaacggtttc ataggtactt gcgggatatg ccagacacgt aatttcatat ttcctccgtc
      541 aaaaaaatcg ccctcacatt ggagggcaaa gaagatttcc aataatcaga acaagtcggc
      601 tcctgtttag ttacgagcga cattgc
//
LOCUS       DAZGOB010000489          623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZGOB010000489 DAZGOB010000000
VERSION     DAZGOB010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.492"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>623
                     /locus_tag="ACHLIF_005611"
     CDS             <1..>623
                     /locus_tag="ACHLIF_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9609359.1"
                     /translation="QQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPA
                     WCLWDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQ
                     QRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVADNEGVGFRYSF
                     SALKDRHTAVEVSYVDPHNGWQTSTELVEDPEAILRYGRNLLKMDAFG"
BASE COUNT          137 a          162 c          203 g          121 t
ORIGIN      
        1 cagcagatga cggtgaacta ccatatccgc ggtcgcatca tccaggtgcc gtcaaactat
       61 gacccggaaa aacgcacgta cagtggtatc tgggacggca gtctgaaacc ggcatacagc
      121 aacaacccgg cctggtgtct gtgggacatg ctgactcacc cgcgctacgg catgggaaaa
      181 cgtctggggg cggcggatgt ggacaaatgg gcgctgtatg ccattgcgca gtactgcgac
      241 cagacggtgc cggatggttt cgggggcaca gagccgcgga tgacctttaa tgcgtacctg
      301 tcacaacagc gtaaggcgtg ggacgttctc agtgatttct gctcggcgat gcgctgtatg
      361 ccggtatgga acgggcagac gctgacgttt gtgcaggacc gtccgtcaga tgtggtgtgg
      421 ccctacacaa acagtgatgt ggtggctgac aatgaaggtg tgggattccg ctacagtttc
      481 agcgccctga aggaccgtca tactgcggtg gaggtcagtt acgtcgaccc gcataacggc
      541 tggcagacct ccacggaact ggtggaagac ccggaagcca tactgcgcta cgggcgcaac
      601 ctgctgaaga tggatgcgtt cgg
//
LOCUS       DAZGOB010000490          623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZGOB010000490 DAZGOB010000000
VERSION     DAZGOB010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.493"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>623
                     /locus_tag="ACHLIF_005612"
     CDS             <1..>623
                     /locus_tag="ACHLIF_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9609360.1"
                     /translation="FNCFFHPLVGLPPVYAAGLAAMQGHHIQANSTYFFRNGGRPSGV
                     IEVPGSITEENAKKLKGNWDSGYTGENAGKTAILSNGAKYSPTTFSPVDAQTVEQLKM
                     TAEIVCSVFRVPAYKIGVGHPPSSDNVEALEQQYYSQCLQTLIESIELLLDEALETGE
                     NESTEFDVTTLLRMDSERRMKTLGESVKNTLLTPNEARKRENLPPLAG"
BASE COUNT          157 a          152 c          190 g          124 t
ORIGIN      
        1 tttaactgtt tttttcatcc gcttgtgggg ctgccgccgg tgtatgccgc aggactggcc
       61 gccatgcagg ggcatcatat tcaggcaaat tcgacgtatt ttttcagaaa tggcgggcga
      121 ccatcaggcg tgattgaggt ccccggcagt attacggaag aaaacgcgaa aaaactgaag
      181 gggaactggg acagcggata tacgggcgaa aatgccggga agacggccat actgagcaac
      241 ggggcaaaat acagccccac gacgttttca ccggtggatg cgcagacggt ggaacaactg
      301 aaaatgacgg ctgaaattgt ctgttcggtg ttccgtgtcc cggcctacaa gattggcgtt
      361 ggccacccgc cttccagtga caacgtggag gcgctggagc agcagtatta ttcccagtgt
      421 ctgcagacgc tgattgagtc cattgagctg ttactggatg aggcgctgga aacgggggaa
      481 aacgagagca cggagtttga cgtcaccacg ctgctgagaa tggacagcga acggcgcatg
      541 aaaacactgg gtgaatcagt gaaaaatacg cttctcacgc ccaatgaggc ccgtaaacgt
      601 gagaacctgc cgccccttgc cgg
//
LOCUS       DAZGOB010000491          622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZGOB010000491 DAZGOB010000000
VERSION     DAZGOB010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.494"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(479..559)
                     /locus_tag="ACHLIF_005613"
     CDS             complement(479..559)
                     /locus_tag="ACHLIF_005613"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9609361.1"
                     /translation="MLRERLMAMQKQLEGAQEYIRTQCIP"
     gene            complement(541..>622)
                     /locus_tag="ACHLIF_005614"
     CDS             complement(541..>622)
                     /locus_tag="ACHLIF_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO9609362.1"
                     /translation="PPAWIMQPVPDWQKPLNGIISSSENG"
BASE COUNT          213 a          146 c           91 g          172 t
ORIGIN      
        1 gtgatcatgt agcataagta acctccgttt atctacagac tgccatcctg aatttaccgt
       61 ccttaataag acaacaataa aaaactaatt atataaaaac aataaaaata atatacaaaa
      121 aaactaaaca acaaagaaaa gcaaatcata tctggcattt taattgaaag agcaattacc
      181 gaacaaaaga cgctgactat atactcaaaa ccaaacaaca attctgccaa tcaggtatca
      241 tggcaacaca cggaattacc atgtttttgc cttctctgcc catacaatac gggcatatac
      301 ttcatactct attgtaatat ttctatccat gcgccccact ccatttacct gtaaataata
      361 ttcaaaatat ttatcacaga aatcgttttt ggccatgaac tgagcacact ataaagtccg
      421 gaactgactc tttgttaaat taccttaacg ttaccagtaa caccttcgta acaaaacatc
      481 acggtataca ctgggtacgg atatattcct gtgctccttc cagttgcttc tgcattgcca
      541 tcagccgttc tctgaggatg aaataatccc gttcagcggc ttctgccagt cggggaccgg
      601 ttgcattatc cacgccggag gt
//
LOCUS       DAZGOB010000492          614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZGOB010000492 DAZGOB010000000
VERSION     DAZGOB010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.495"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..>614
                     /locus_tag="ACHLIF_005615"
     CDS             2..>614
                     /locus_tag="ACHLIF_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO9609363.1"
                     /translation="MHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNR
                     LFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQIL
                     NEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSK
                     DLANLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNV"
BASE COUNT          242 a          107 c          104 g          161 t
ORIGIN      
        1 tattcatcac cagatcattg aaaaccaact ggatacagaa acaataacta ttagttatga
       61 atttatcctc tgtcaacatc ttatcgcaaa taccgagttc agctatttgg cactccctga
      121 aaattataac cgtttgtttt taccaaattc aaaaaaccaa acaaataatc gtttcaaaac
      181 gttaaacagc aaagctattg gcaggctact tgctgctggt ggcgtataca atgggaacat
      241 tgaaggattc agagatactg cggaaaaact gggtggagat gcaataaaag gctatgatca
      301 aatactaaat gaaaaaacag cgggcatagc gatagcaaca gcatctattc ttttaacaaa
      361 gcgttctaat gttgatacat atacagaaat aaatagttac ttaggcaaac ttagaggtca
      421 acaaaaactt cttgatggta tagacataat agaaataata tacattaaga gaccttcaaa
      481 agacttagct aacttacgaa aggagtttaa taaaactgta agaaaaaatt ttcttatcaa
      541 acttgcaaaa acctccgaag catctggaag attcaacgcc gaagaccttt taagaatgag
      601 aaagggcaat gttc
//
LOCUS       DAZGOB010000493          611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZGOB010000493 DAZGOB010000000
VERSION     DAZGOB010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.496"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..526
                     /locus_tag="ACHLIF_005616"
     CDS             <1..526
                     /locus_tag="ACHLIF_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO9609364.1"
                     /translation="GLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQFPSGKG
                     FKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVNTGALLT
                     MSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSAEQPQSI
                     TSDACTVTVNGAGG"
     gene            532..>611
                     /locus_tag="ACHLIF_005617"
     CDS             532..>611
                     /locus_tag="ACHLIF_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO9609365.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEW"
BASE COUNT          147 a          138 c          188 g          138 t
ORIGIN      
        1 cggtctgggg gcgtcgtccg agatttccat gtcgggtaat ttttatctga atcaggccca
       61 gaacgccctg cgtgatgcct atgacaatga cacggtgtat gcgtttaagg tgcagtttcc
      121 gtccggtaag ggctttaagt tcctggcgga agtgcgtcag cacacctggt catccggtac
      181 caacggcgtg gtggctgcaa cgttttcact tcgcctgaag ggtaaaccgg tgtcctatgt
      241 ggtaccgctg gcgtttgtga aaaatctgga gaagacactt accgtgaata ccggtgcgct
      301 gctgacaatg tcagtcagtg tcaacggggg aacgccgcct tataaacacg cctggaagaa
      361 ggatggtcag ccggtagagg gacagactac tgacactttc agtaaagcca atacgcagtc
      421 aggtgataag ggggcttata cctgcgaggt aacggattct gcagaacagc cgcagagcat
      481 tacctctgat gcgtgtacag taacggttaa tggtgcgggc ggataaggct tatggcaaaa
      541 gatctgaaaa cactggcgct ggccagactg tcggggttcc gtcataaaac ggtgaaggtg
      601 ccggaatgga g
//
LOCUS       DAZGOB010000494          610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZGOB010000494 DAZGOB010000000
VERSION     DAZGOB010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.497"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>610
                     /locus_tag="ACHLIF_005618"
     CDS             <1..>610
                     /locus_tag="ACHLIF_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9609366.1"
                     /translation="ARKNGTYSGSQQNTGASDTTAQPEPVKVTADEVNKIMQAANISQ
                     PDADKLLAASRGEFVAGISDPNDPKWVKGIQTRDSVNQNQHESERNYQKAEQNSPNAL
                     QNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCGAVMGDATYQETFDEEYQVEVQE
                     DDPEKMEGAEHPHKENTGGNQHHASDNETGETADHSIKVNGH"
BASE COUNT          202 a          151 c          161 g           96 t
ORIGIN      
        1 tcgcccgcaa aaacggtaca tactcaggtt cacaacaaaa tactggtgca tctgacacga
       61 ccgcacaacc agagccggta aaagttaccg ctgacgaagt aaacaaaatt atgcaggcag
      121 ccaatatcag ccagcctgac gccgataagt tgcttgctgc ctctcgcgga gaatttgttg
      181 cagggattag cgacccgaat gatccgaaat gggttaaggg gatccagacc cgcgattctg
      241 taaaccagaa ccagcatgaa tcggaacgga actaccaaaa agcggaacaa aacagcccaa
      301 atgcgttaca aaacgagcca gaaacgaaac agcctgaacc agtggcgcaa caggaagtgg
      361 aaaaagtctg caccgcctgc ggtcagaccg gcggcggcaa ctgccctgat tgtggcgcgg
      421 tgatgggcga cgcaacatac caggaaacat tcgatgaaga gtatcaggtt gaagttcagg
      481 aagatgatcc ggagaaaatg gaaggcgctg aacatccaca caaggagaac actggcggca
      541 atcagcatca tgccagcgat aatgaaactg gcgagacggc agatcactca attaaggtga
      601 acggtcatca
//
LOCUS       DAZGOB010000495          602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZGOB010000495 DAZGOB010000000
VERSION     DAZGOB010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.498"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>602
                     /locus_tag="ACHLIF_005619"
     CDS             <1..>602
                     /locus_tag="ACHLIF_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO9609367.1"
                     /translation="DLQKQFGENRISIKDVNSLTSLSKSENNHNYQTEMLLRWNYPAA
                     SDLLRMYILKEHGGIYTDTDMMPAYSKQVIFKIMMQTNGDNRFLEDLKLRRAISDGVL
                     RYVNNQNIDEVNYNEISDADKNIIKKILTEISKMPEDSIFTKINTRIPRDTMPILRRY
                     HLWPDGWNIRGLNGFMLSHKGSEVIDAVIAGQNQAYRELR"
BASE COUNT          216 a          102 c          111 g          173 t
ORIGIN      
        1 gatttacaga aacaatttgg tgaaaaccgt atttcaatta aagatgttaa ctctttaacc
       61 tctctttcaa aatcagaaaa taatcacaat tatcaaactg aaatgttgct tcgatggaac
      121 tatcctgccg cctcagacct gctcaggatg tatatcctta aagagcatgg tggtatttat
      181 acagacacag acatgatgcc tgcatactct aaacaagtaa tttttaaaat tatgatgcag
      241 acaaacggag ataatcgttt cttggaagat ttgaaactac gtcgtgcaat atctgatggt
      301 gtattaagat atgttaataa ccaaaatatt gatgaagtta actataatga aatcagtgat
      361 gcagataaaa acattatcaa gaagatatta acagaaatat ctaaaatgcc agaagatagc
      421 atttttacta agatcaatac aaggattcct cgagacacaa tgcccatcct tcgtcgttat
      481 cacctatggc ctgatggatg gaatattcgt gggctcaatg gattcatgct atcacataaa
      541 ggtagtgaag tgattgatgc tgttatcgca ggccagaatc aggcttacag ggaactaaga
      601 ag
//
LOCUS       DAZGOB010000496          602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZGOB010000496 DAZGOB010000000
VERSION     DAZGOB010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.499"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>602
                     /locus_tag="ACHLIF_005620"
     CDS             <1..>602
                     /locus_tag="ACHLIF_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9609368.1"
                     /translation="GQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAE
                     LRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETLEYK
                     RQLAELGDKVEYQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRL
                     RDVYGDNPAALAKATSALKNTWSAEEQLRGSWMAGLKSGWG"
BASE COUNT          158 a          148 c          210 g           86 t
ORIGIN      
        1 gggcagcagg aagccgtcag ccgtctggct gaagagctga caggtaaaaa gcatacatca
       61 ccgaaaacgc gctctgccgg ggagagggaa gaggagcagg caagagaggc tctgcttgcc
      121 cttgaagctg agctcaggac gctggaaaaa cacagcggtg cgaatgagaa aatcagccgg
      181 cagcgccgtg atttatggaa ggcggaaagt cagtatgcgg tcctgaaaga ggctgccacg
      241 aaacgacagt tatctgagca ggaaaaatcc ctgctggcgc ataaagacga gacgctggag
      301 tacaaacgcc agctggctga gctgggcgac aaggttgaat accagaaacg cctgaatgag
      361 ctggcacagc aggcggtgcg gtttgaagag cagcagagcg cgaagcaggc cgccatcagc
      421 gcaaaagccc gcggtctcac tgaccgtcag gcgcagcggg agtctgaagc gcagcgtctt
      481 cgggacgtgt acggtgataa tccggctgcg ctggcgaagg ccacatcggc actgaagaac
      541 acctggtctg cggaggagca gcttcgtgga agctggatgg ccgggctgaa gtccggctgg
      601 gg
//
LOCUS       DAZGOB010000497          602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZGOB010000497 DAZGOB010000000
VERSION     DAZGOB010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.500"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>602
                     /locus_tag="ACHLIF_005621"
     CDS             <1..>602
                     /locus_tag="ACHLIF_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9609369.1"
                     /translation="GQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAE
                     LRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSWQEKSLLAHEKETLEYK
                     RQLADLGDKVEHQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRL
                     RDVYGDNPQALARVTGALKQTWADEDMLRGDWLAGLKSGWG"
BASE COUNT          155 a          150 c          212 g           85 t
ORIGIN      
        1 gggcagcagg aagccgtcag ccgtctggct gaagagctga caggtaaaaa gcatacatca
       61 ccgaaaacgc gctctgccgg ggagagggaa gaggagcagg caagagaggc tctgcttgcc
      121 cttgaagctg agctcaggac gctggaaaaa cacagcggtg cgaatgagaa aatcagtcgg
      181 cagcgccgtg atttatggaa ggcggaaagt cagtatgcgg tcctgaaaga ggctgccacg
      241 aaacggcagt tatcctggca ggaaaaatcc ctgctggccc atgagaaaga gacgctggag
      301 tacaaacgcc agctggctga cctgggcgac aaggttgaac accagaaacg cctgaatgag
      361 ctggcacagc aggcggtgcg gtttgaagag cagcagagcg cgaagcaggc cgccatcagc
      421 gcaaaagccc gcggtctcac tgaccgtcag gcgcagcggg agtctgaagc gcagcgtctt
      481 cgggacgtgt acggtgataa tccgcaggcg ctggcccggg tcactggggc actgaaacag
      541 acatgggcgg atgaagacat gctgcgcggt gactggctgg ccgggctgaa gtccggctgg
      601 gg
//
LOCUS       DAZGOB010000498          595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZGOB010000498 DAZGOB010000000
VERSION     DAZGOB010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.501"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>595
                     /locus_tag="ACHLIF_005622"
     CDS             <1..>595
                     /locus_tag="ACHLIF_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFO9609370.1"
                     /translation="SNKIGQRVYTVVSEMSIDAWITKEPQPFVYRRSGAYQKLTIGSD
                     WGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITNKASSYGVP
                     PDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATCNQLLKSLYY
                     DVEVLFDWINDGQKFESIHPKGIIPEKIITKRSERTD"
BASE COUNT          168 a          113 c          139 g          175 t
ORIGIN      
        1 tgtcgaataa aataggtcag cgtgtttata ctgttgtctc tgaaatgtcg attgatgcat
       61 ggataacaaa agaaccgcag ccttttgttt atcgccgtag cggcgcttac cagaaactca
      121 ccattggctc agactggggt caactgtttg actgtgcctg gatgcgagtt tatggtaaac
      181 gtccagcaga taaatttaga cacaaactcg tcgctctcgt tgatatcggt ggcgaaggtc
      241 ttattgtgga taagaatggt tcacccatct gcggcatcac gaataaagca tcatcttatg
      301 gcgtaccgcc agataaacca ggtaaatggg tggttgacct ttctctcgtt agtgaaaatg
      361 acgaggtcga gttttggatt gatgctgcgt gcaacgattt atttggctat gtcactaatg
      421 gagggattat tactgatgtt catatcgcga catgtaatca actactgaaa tccctttatt
      481 atgatgttga agtgcttttc gactggatta atgatggtca gaagtttgaa tccatacatc
      541 ctaaaggtat tatcccagaa aaaattatca ccaaaagaag cgagcgtact gacga
//
LOCUS       DAZGOB010000499          591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZGOB010000499 DAZGOB010000000
VERSION     DAZGOB010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.502"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>591
                     /locus_tag="ACHLIF_005623"
     CDS             <1..>591
                     /locus_tag="ACHLIF_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9609371.1"
                     /translation="ALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTLWYYDESD
                     RITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDKGRLTGERQTVENPE
                     TGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGDTPLLEYTRDR
                     MHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQHLN"
BASE COUNT          141 a          175 c          191 g           84 t
ORIGIN      
        1 gcgctggacc ggttggtaca gcagggcggc tttgacgggc ggacgcaacg ttatcattat
       61 gacctgaccg gaaaactcac gcagagtgaa gatgaggggc ttgtcaccct ctggtactac
      121 gatgaatcgg accgtatcac tcaccgcacg gtgaacggcg aaccggcaga gcagtggcag
      181 tatgatgacc acggctggct gacagacatc agccacctga gcgaaggcca ccgtgtcgct
      241 gtccactacg gctacgacga taagggccgc ctgaccggcg aacggcagac ggtggagaac
      301 ccggagacgg gggaactgct gtggcagcat gagacaacac acgcctacaa cgaacagggg
      361 ctggcaaacc gcgtcacgcc ggacagcctg ccgccggtgg agtggctgac ctacggcagc
      421 ggttatctgg cgggcatgaa gcttggcgac acgccgctgc tggagtacac gcgcgaccgc
      481 atgcaccggg agacggtgcg cagctttggc agcatggcag gcagtaatgc cgcatataaa
      541 ctgaccagca catacacccc cgcaggccag ttacagagcc agcacctgaa c
//
LOCUS       DAZGOB010000500          590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZGOB010000500 DAZGOB010000000
VERSION     DAZGOB010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.503"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>590)
                     /locus_tag="ACHLIF_005624"
     CDS             complement(<1..>590)
                     /locus_tag="ACHLIF_005624"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9609372.1"
                     /translation="MFASLLIIFAIAFFFYKDYASVGRNNSSLNKEIIPTNYIYSGFK
                     YVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARSQNYALNGYSRGTNDFT
                     KKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVNLL
                     WIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCY"
BASE COUNT          185 a          117 c           94 g          194 t
ORIGIN      
        1 tcataacatg agtttttctt gcataatgta ttatctgaat ttgatggctc aatattaatt
       61 gtagggattc gcttacatac acctttacat cccccgtcat tttcaatcca gagtaaattt
      121 acccccgttc tgtacagaat atccagtaca ttttccgaat ttaccgcttt gcgggaattg
      181 aactcttttc gcttcatatc tgaaaacatg catggaacag atatggctgt ggatgtgcca
      241 cacgactgca cattatgaaa ggatataagt tcattatatt tctttgtaaa atcattggta
      301 ccacgagaat atccattcag agcatagttt tgacttcttg ctgtctcccc gacaactaaa
      361 aacataatta ccggcttttg tttttcattt attgtgcggg atgcatccgt tccagtctga
      421 cgaaactcgc caggagaaac aaaaaaatcc cttacatatt taaacccact gtagatataa
      481 ttagtaggta ttatttcttt atttaatgaa gagttgtttc tgccaactga tgcataatcc
      541 ttataaaaga aaaacgcaat cgcaaatata atcaagagag atgcaaacat
//
LOCUS       DAZGOB010000501          587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZGOB010000501 DAZGOB010000000
VERSION     DAZGOB010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.504"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..399
                     /locus_tag="ACHLIF_005625"
     CDS             <1..399
                     /locus_tag="ACHLIF_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609373.1"
                     /translation="PFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQE
                     QVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSII
                     RQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            475..>587
                     /locus_tag="ACHLIF_005626"
     CDS             475..>587
                     /locus_tag="ACHLIF_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609374.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDT"
BASE COUNT          147 a          144 c          149 g          147 t
ORIGIN      
        1 ccatttggtg cactgacagg gcgtgtggtt ggcggtgttc tggggggaat gattgcctcc
       61 gctgtatcag gaaaaattgc cggagcgctg gttgaagaag atcgcgtcaa aattctggca
      121 atgattcagg agcaggtgac atggcttgcc ggcagtttcc tgctgaccgg acatgagatt
      181 gaaaatctga acgagaatct ggcccgtgtt atcgatcaga atgctctgga gatcattttc
      241 gccgccggta tacaacaacg ggccgcgacc aatatgttaa tcaaaccact ggtggtcagt
      301 atcatcaggc aacgccccgt catggaatat gatgcatccc atctcggcaa tatggttaac
      361 cgactggaag aagcattacc cccggagtta ccggcataac ggctattctg cgccttttac
      421 gctttatcac ctgatttgtc agtgtcatcg tcatgactca gagaggtaaa taccatgatt
      481 cacctgttca aaacctgcat gattaccgcc ttcattctgg ggttaacgtg gtctgcccca
      541 ctccgggcac aggatcaacg ttacatcagt atacgcaata cagatac
//
LOCUS       DAZGOB010000502          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZGOB010000502 DAZGOB010000000
VERSION     DAZGOB010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.505"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..533)
                     /locus_tag="ACHLIF_005627"
     CDS             complement(<1..533)
                     /locus_tag="ACHLIF_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9609375.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSASGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTA"
BASE COUNT          130 a          185 c          160 g          110 t
ORIGIN      
        1 tctgccgtgc tgatctcctg agaaaccacg cgagacccca cgcgcatttc cccgtacaga
       61 acaggcagaa cattgccctg ggcaaccatg ttatccagtg aggagaaata ggtgttctgc
      121 ttaccgttat ccgttgtctg tgtacgggga gttctggctt tcggtgccag catctgcgcc
      181 acaccaccga gcaccatact ggcaccgaga gaaaacagga tgccggtcat accaccggcc
      241 ccaatggctg ccccccatgc tgcaagggtg gctccggcgg taaagaatga tccggcaatg
      301 gctgcagccc ccaggacaat ctggaatacg ccacctgact tggccccggc gactctggga
      361 acaatatgaa tcacagcgcc atcaggcaga gtctcatgta actgcgccgt taacccggac
      421 gcgctgacgt cccgcccggc aatccgtacc tgataccagc cgtcgctcag tttctgacga
      481 aacgccggga gctgtgtggc cagtgcgcgg atggcttcag cccccgtttt cacacgaagg
      541 tcgatgcggc ggccaaatcg ttgcaaatcc ccgtaaaggc agatg
//
LOCUS       DAZGOB010000503          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZGOB010000503 DAZGOB010000000
VERSION     DAZGOB010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.506"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>581
                     /locus_tag="ACHLIF_005628"
     rRNA            <1..>581
                     /locus_tag="ACHLIF_005628"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          164 a          127 c          177 g          113 t
ORIGIN      
        1 ataatgggtc agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga
       61 aaccgagtct taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc
      121 catgggcagg ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa
      181 aattagcgga tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt
      241 ctccccgaaa gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt
      301 tcggcaaggg ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt
      361 tatcacggga gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac
      421 cgccagctaa ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag
      481 ccaggatgtt ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga
      541 gtcggcctgc gcggaagatg taacggggct aaaccatgca c
//
LOCUS       DAZGOB010000504          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZGOB010000504 DAZGOB010000000
VERSION     DAZGOB010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.507"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..563
                     /gene="tnpA"
                     /locus_tag="ACHLIF_005629"
     CDS             <1..563
                     /gene="tnpA"
                     /locus_tag="ACHLIF_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO9609376.1"
                     /translation="VPKSTVCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPV
                     VRPESTVISEAPVVKKRPRRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFN
                     WRHQYRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPA
                     GTLRLKGKLTPALLQTLIREIKGSSH"
BASE COUNT          143 a          165 c          148 g          125 t
ORIGIN      
        1 gtgtaccaaa atcaacagtt tgtagtatgt tcgtgcgctt tcggagagct ggcctttcgt
       61 ggcctttgcc cgcaggcatg tcggagcagg aacttgatgc ctgcctttac ggacaatttt
      121 ccacggtacc agtcgtacgt cctgaaagca ccgttatatc cgaagccccc gtggtaaaaa
      181 aacgtccccg gcggcccaac ttcccttatg agtttaaaat cgccttagtg gagcagtcac
      241 tgcagcccgg agcctgtgtg gcgcagatcg cccgggaaaa cggaatcaac gataacctgc
      301 tcttcaactg gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc
      361 cggcactgct tcccgtgacg ttaacgccgg agccggataa taaaatcccg gcccccgcac
      421 aggaaccaga gcagataaat acaccgtccg acagtctgtg ttgtgagctg gttctgccgg
      481 ccggaactct caggcttaaa ggtaaactga cgccggcgtt attacagaca cttatccgcg
      541 aaataaaagg gagcagccac tgatgatatc tctccctgca g
//
LOCUS       DAZGOB010000505          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZGOB010000505 DAZGOB010000000
VERSION     DAZGOB010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.508"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            193..345
                     /locus_tag="ACHLIF_005630"
                     /pseudo
     CDS             193..345
                     /locus_tag="ACHLIF_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(370..567)
                     /locus_tag="ACHLIF_005631"
     CDS             complement(370..567)
                     /locus_tag="ACHLIF_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609377.1"
                     /translation="MRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAW
                     ILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          171 a          109 c          110 g          178 t
ORIGIN      
        1 tatgttattc atttgcattt gccttggtta tatttaagct caatatcaat tcgagaacaa
       61 atgctagggt aaaaaaatat ttaaaaaaga agtaaacttc acattttttc attacatctc
      121 tgaagagaac ctcgttaatg attggtcttg ctcattgtta ataacaaaat gttgaattta
      181 ccgattagga aactttttcc tacgcggatt gcctccagct atggcatgaa ctttgagttt
      241 atgccagaac tgaagtgggg cttcggctac cctggcgcga ttatctttat gatcctcgcg
      301 ggcctgacac cgtatctgta ctttaagcgt aagaactggc tgtaactata cgatgtcgct
      361 gtgagtaagt tagtgtgctg acagcgacat tttagccgag ggaatcgcag ataacataat
      421 tgttgtgaat aatatccatg ccacgttgag ttggacgctt aatgactcac atctcacccc
      481 ttcaattgct gcagagataa tagaaaaaag tgtcaagccc aatgaaatca atacaaagta
      541 gattgcctta catccagtat ctcttatt
//
LOCUS       DAZGOB010000506          563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZGOB010000506 DAZGOB010000000
VERSION     DAZGOB010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.509"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            20..520
                     /locus_tag="ACHLIF_005632"
                     /pseudo
     CDS             20..520
                     /locus_tag="ACHLIF_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          199 a           98 c           87 g          179 t
ORIGIN      
        1 ttagagtaca ggtgggcata tgcaagtctt ttccagtgat gtctacttca ctgtaggcac
       61 taatgcatta ttagcatccc aaaaagaata ttacagcgat ttagttgcgt tggttgatct
      121 tggacattcg ttcgttgtta ttgatgagca tcaacatcga aatctcaaac caaatacaga
      181 acctgtaaac atccttttga gcaacaattt catcaggatc aataagaata ttacattatc
      241 tgatttaacg catttcctgg tttcaaatct tcatacacaa aatgtgtatt ccacacaaga
      301 accacttacc catgatgaga tagatatcct gcggttatgt gtttcttact cgttaaaaca
      361 aatagccatt attaaaggaa ttgattataa aacaatatca tatcacaaaa taagagccct
      421 aaataaactt aacataaaag gtactgttta gttgtttata gcactctgtg aatgggataa
      481 acattatttt aaactccaat cttgcgtaag agaaagttga tatgtcatta gtaggatata
      541 atagtaacat cataataacc atc
//
LOCUS       DAZGOB010000507          552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZGOB010000507 DAZGOB010000000
VERSION     DAZGOB010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.510"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>552
                     /locus_tag="ACHLIF_005633"
     CDS             <1..>552
                     /locus_tag="ACHLIF_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609378.1"
                     /translation="KVDNNEDYKSLISATLGYPTRINVKDVRGWDTRFDINFQMEELT
                     AEQAIIEIRSVEAFSTGSIIFEDEKEDVSFKCEFFVSPIALNAPRDLASFRICSDFFD
                     LLVGIKNNKLKIDFESYDKPFHLADIRELLLLMKILNAPSGDLKLTIKNNEGNETSFL
                     ASDNSFSANPITNEIIKAEQIALA"
BASE COUNT          192 a           83 c          104 g          173 t
ORIGIN      
        1 aaagttgata ataatgagga ttataagtct cttattagtg ccacgcttgg atatccgacc
       61 agaataaatg taaaagatgt tcgcggttgg gatactcgat ttgatattaa ttttcagatg
      121 gaagaactga cagctgaaca agcaataatc gaaataagat ctgttgaagc attctcaaca
      181 ggctctataa tattcgaaga tgaaaaagaa gatgtctctt ttaagtgtga atttttcgtt
      241 tctcctattg cacttaatgc tccaagggat ctggcaagct ttagaatttg ctcggacttt
      301 ttcgatttac tggttggtat taaaaataac aaattgaaaa tagattttga atcatatgat
      361 aagccttttc atttagctga cattagagaa ttactgttat taatgaagat attaaatgcc
      421 ccaagcggag atctgaagtt aacaataaaa aacaatgagg gtaacgagac atccttttta
      481 gcctcggata attcattttc tgcgaatcca attactaacg aaataataaa ggcagaacag
      541 atagccttgg ct
//
LOCUS       DAZGOB010000508          551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZGOB010000508 DAZGOB010000000
VERSION     DAZGOB010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.511"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..155
                     /locus_tag="ACHLIF_005634"
                     /pseudo
     CDS             39..155
                     /locus_tag="ACHLIF_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(249..380)
                     /locus_tag="ACHLIF_005635"
     CDS             complement(249..380)
                     /locus_tag="ACHLIF_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO9609379.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
BASE COUNT          154 a          164 c          104 g          129 t
ORIGIN      
        1 aacagcccct ccggagaggg gctggagagt ggcgctatgt gccattgcat ggtgccgggt
       61 gcctcccggt gaattcagta ccagcacctg aatccgcgat tatcccatat acctactcgc
      121 tgattgcccc tccgcacagg gggattcacc atgccagttt cttttaacaa actccccgca
      181 aaccagacaa cagtcaaccg cctgaattgt gaagtattta aaaatttctc ccgctaactg
      241 atacccggct aacagtctgg cgttttcttt ttcagcaacg ggaaagcaac aaccaccaca
      301 cccgccacca gcacaccgtc agccagcact gacattatcc ggctgctgca atgccattca
      361 caaaaacagt aagcaatcac tttttaccgt aacaggtgat aatccagata tgtatctacc
      421 ccagatgagt aatccgaagt tcatccatac cacaggtcct ggctattctg ttgtactcct
      481 gaacaagagc aaataattct gaattagcaa ccatgaactc atcgcaaacc ctctgtatag
      541 catcactatt c
//
LOCUS       DAZGOB010000509          551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZGOB010000509 DAZGOB010000000
VERSION     DAZGOB010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.512"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>551
                     /locus_tag="ACHLIF_005636"
     CDS             <1..>551
                     /locus_tag="ACHLIF_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9609380.1"
                     /translation="RPALDSWDAEYMQENRWDEESASFVPVETESDPINVTFDKLAPE
                     VQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLH
                     AIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARARANQQHTENS
                     TGKISPVIAAIHREYKQTWKTLD"
BASE COUNT          166 a          128 c          142 g          115 t
ORIGIN      
        1 cgccctgccc tcgattcctg ggatgctgaa tatatgcagg aaaaccgctg ggatgaggag
       61 tctgcttcct ttgtcccggt tgagactgaa tcagatccga taaacgtcac ttttgacaag
      121 ctggcccctg aagtacagaa cgctgtcatg gttaagttcg acacatgtga aaacatcacc
      181 gttgatatgg tgattagcgc gcaggaactg ttgcaggaag acatggcaac attcgacgga
      241 catatcgttg aagcgttgat gaaaatgcca gaagttaacg ccatgtatcc ggagcttaag
      301 ctgcatgcca tcgggtgggt taagcataaa tgtaagccag gtgccaaatg gcccgaaatt
      361 caggcagaga tgcgcatctg gaaaaaacgt cgcgaaggtg aacgcaagga aaccggaaaa
      421 tacacgtctg ttgttgatct cgcccgcgcc agagccaatc aacagcacac tgaaaattca
      481 acaggaaaaa tcagcccggt cattgctgcc attcatcgcg aatacaagca gacatggaaa
      541 acactggatg a
//
LOCUS       DAZGOB010000510          550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZGOB010000510 DAZGOB010000000
VERSION     DAZGOB010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.513"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..346)
                     /locus_tag="ACHLIF_005637"
     CDS             complement(<1..346)
                     /locus_tag="ACHLIF_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO9609381.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFF"
     gene            complement(336..550)
                     /locus_tag="ACHLIF_005638"
                     /pseudo
     CDS             complement(336..550)
                     /locus_tag="ACHLIF_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
BASE COUNT          192 a          116 c           72 g          170 t
ORIGIN      
        1 caaaaaagct aaccaactca ggttcagaat attttatcaa atatgattta taaccactca
       61 tgcctgtctc aacaaaatta acaaccgatg ctacattatt gaatgaaaga ttctcattct
      121 gactgttatt atatatttcc ttcccaaccg gcatcattac gaacattgac aatagcaaag
      181 caaccccatt aagtgtcata tttgatggca cttgctgaag tcccagcgcg tttcggacaa
      241 taacaaaaac aatcgagaat ttaataaaac atgtcccgga tgctattata aacggcaata
      301 gtgtaaataa tgataatatc gctatcagcg aaatgctatt tgacattatt attccctgat
      361 aaccaagaat gaatttctac gcccaattta tcatccactt caacaagttc gccatagcca
      421 gttagcgcac catttacacg tatctctata ttttctcaga ttcaggaagc agagatatta
      481 ttctttttgc acagagatcg tctatttcat aaagattaat tattttctta ccaagaacaa
      541 actcaatttt
//
LOCUS       DAZGOB010000511          547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZGOB010000511 DAZGOB010000000
VERSION     DAZGOB010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.514"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>547
                     /locus_tag="ACHLIF_005639"
     CDS             <1..>547
                     /locus_tag="ACHLIF_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO9609382.1"
                     /translation="IAESKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDD
                     ENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSS
                     EACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPG
                     QLFMDPKRMIEACDENTIGVVP"
BASE COUNT          141 a          137 c          148 g          121 t
ORIGIN      
        1 ctatcgcgga gtcaaaacga tttccgctgc acgaaatgcg cgatgatgtc gcattccaga
       61 ttatcaatga tgaattatat cttgatggca acgctcgtca gaacctggcc actttctgcc
      121 agacctggga cgacgaaaac gtccataaat tgatggattt gtcgatcaat aaaaactgga
      181 tcgacaaaga agaatatccg caatccgcag ccatcgacct gcgttgcgta aatatggttg
      241 ccgatctgtg gcatgcgcct gcgccgaaaa atggtcaggc cgttggcacc aacaccattg
      301 gttcttccga ggcctgtatg ctcggcggga tggcgatgaa atggcgttgg cgcaagcgta
      361 tggaagctgc aggcaaacca acggataaac caaacctggt gtgcggtccg gtacaaatct
      421 gctggcataa attcgcccgc tactgggatg tggagctgcg tgagatccct atgcgccccg
      481 gtcagttgtt tatggacccg aaacgcatga ttgaagcctg tgacgaaaac accatcggcg
      541 tggtgcc
//
LOCUS       DAZGOB010000512          536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZGOB010000512 DAZGOB010000000
VERSION     DAZGOB010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.515"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            101..>536
                     /locus_tag="ACHLIF_005640"
     CDS             101..>536
                     /locus_tag="ACHLIF_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO9609383.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSAF"
BASE COUNT          127 a          161 c          146 g          102 t
ORIGIN      
        1 tatcgactgg cggcagccta aaagactgct tacgtccctg actatgttgt aagcctcttt
       61 atcctggtcg acgctgaatg agcctggtaa tatacccggt atgagcagct cacttcctga
      121 cgatatcaat gcactgaaac gtctccttgc cgaacaggag gcgctgaacc gtgccctgct
      181 ggaaaagctg aacgagcgtg aacgcgaaat agaccatctg caggcacagc tggataagct
      241 gcgccggatg aacttcggca gccgctccga aaaagtctcc cgtcgtatcg cacagatgga
      301 agctgacctg aaggcacttc agaaagaaag tgataccctt accggtcggg ttgacgaccc
      361 ggccgtgcag cgcccgctgc gtcaaacccg cacccgcaaa ccgttccccg aatcactccc
      421 ccgcgatgaa aaacggctgc tgccggcagc atcatgctgc ccggaatgtg gaggctcact
      481 gagctatctg ggtgaggatg ccgccgaaca gctggagttg atgcgcagcg ccttcc
//
LOCUS       DAZGOB010000513          534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZGOB010000513 DAZGOB010000000
VERSION     DAZGOB010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.516"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..283)
                     /locus_tag="ACHLIF_005641"
     CDS             complement(<1..283)
                     /locus_tag="ACHLIF_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609384.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNH"
BASE COUNT          169 a           98 c          105 g          162 t
ORIGIN      
        1 catgattcag gtaatatgta gagatagtcc cgttaacagt gtcaccatta aaggtggtat
       61 cgaaatcacc gtttgtgttg ttatagtaga caaagccact ataattcaca tagttaccat
      121 ctacagtacc cggtgcatcc atattgctcc agtcaagagc ggtagttgga ttgtcgtaat
      181 aagtccaggt gtcataagta gttgcattag cctgagaggt gatagccaga gtgcaagcca
      241 atgctaattg tgatactaca agtttctttt tccaggagtg catttgtcat ccctcctcag
      301 ggacgtaata agttgatcca tcaattatca atgcatagaa aacatattat gtttcgcgct
      361 aatgattatg ctgcgttaca gacgaatggt tcaattattc tttcccctaa gtccgaatgt
      421 ggacatatat aacctatgga gaaaaacacg catcaaattg ataaaagggc gatatttcaa
      481 catgttgcag ataatgttat ttttgataca tacgcgttat aaatatcaaa aaca
//
LOCUS       DAZGOB010000514          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZGOB010000514 DAZGOB010000000
VERSION     DAZGOB010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.517"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            26..>530
                     /locus_tag="ACHLIF_005642"
     CDS             26..>530
                     /locus_tag="ACHLIF_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9609385.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAAKKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGN"
BASE COUNT          154 a          108 c          129 g          139 t
ORIGIN      
        1 ttacgaagag atcaggatga tgacgatgaa aaaaagtgta ttgacggcgt ttataactgt
       61 ggtatgtgca acgtccagcg ttatggcagc tgatgataat gctatcacgg atggcaaagt
      121 aacatttaat ggtaaagtta ttgctccagc ttgtaccctg gtagctgcga agaaagattc
      181 agtagtcaca ttgccaaatg tcagcgccac caagttgcaa actaatggag cagtctctgg
      241 cgttaaaact gatgtaccaa ttgccttaga aggctgtgat gttactgtaa ctaaaaatgc
      301 gacgtttacc tttagtggta cggctgacgg cgtgcaacct acagcatttg ccaaccaggc
      361 taccaccgat gcagcgacga atgttgctct acaaatgtat ctgccagatg gttctacatc
      421 ggtaactccc ggtaccgaaa cgtcgaatat tcagttagcg gattctgctg agcaaacagt
      481 gacctttaaa gttgattata tcgctacggg gaaagcgact tctggtaatg
//
LOCUS       DAZGOB010000515          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZGOB010000515 DAZGOB010000000
VERSION     DAZGOB010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.518"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..380
                     /locus_tag="ACHLIF_005643"
     CDS             <1..380
                     /locus_tag="ACHLIF_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO9609386.1"
                     /translation="PLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLSAIPVKINEKA
                     RYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQLPLPLASTLLE
                     RITFYAKKNRDELDKISCKWCCD"
BASE COUNT          180 a           84 c           96 g          170 t
ORIGIN      
        1 ttccattgtt ctgtttattc agtggttatc acttttatcg agaaaggttt gcagaacgcg
       61 gattttttta taaggttcct gctgtgctaa gggattattt atcagctata ccggtgaaaa
      121 taaatgaaaa ggcacgatac aaacccggca tcgctagcta tcacaatatc atcacatgtg
      181 gtttttcaac gttgtcaccc tatataaggc aacaaccatt ggcaatgcag caacgattta
      241 atttattatt tcctgatttt gttgatcaca tacaattgcc gttaccactt gcatcaactc
      301 ttttagagag aattacattt tatgcgaaaa aaaatagaga tgagcttgat aaaatctcct
      361 gcaaatggtg ttgtgattaa aagaaaaatt tctgatggat tgaaataaat agtttctctt
      421 aaagaaagta tccttcttga aaccagcgca aaaattcaga gcattgaggt aaagagagaa
      481 gaaaaattta ttcaaggtta ttatgatggc tatacaaaag gcattattga
//
LOCUS       DAZGOB010000516          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZGOB010000516 DAZGOB010000000
VERSION     DAZGOB010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.519"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>528
                     /locus_tag="ACHLIF_005644"
     CDS             <1..>528
                     /locus_tag="ACHLIF_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9609387.1"
                     /translation="AEYMQENRWDEESASFIPVEPESDPMNVNFDKLSLEVQNAVLVK
                     FGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEINAMYPERKLFAIGWVKHK
                     CNPGAKWPEIQAELRNWKKRQDAERKETGKYTSVVDLASARVNQQNTENSAGKINPVT
                     AAICREYKQTWKTLD"
BASE COUNT          166 a          120 c          130 g          112 t
ORIGIN      
        1 tgctgaatat atgcaggaaa accgctggga tgaggaatcc gcttccttta ttccggtcga
       61 accagaatcc gatccgatga acgtcaattt tgacaagctg tcccttgaag tacagaacgc
      121 ggtcctggtt aagttcggta catgtgaaaa catcaccgtt gatatggcga ttgacgcgca
      181 ggaattactg caggaagacg ttgctacctt tgacgggcat atcgttgaag cactgatgaa
      241 aacgcctgaa attaacgcta tgtatccgga acgcaaactg ttcgctatcg gatgggttaa
      301 acacaaatgt aatccgggtg ccaaatggcc cgaaattcag gctgaattac gtaactggaa
      361 aaaacggcag gacgcagagc gcaaagagac tggaaaatac acgtctgttg ttgatctcgc
      421 cagcgccaga gtcaatcaac agaacactga aaactcagca ggaaaaatca acccagtcac
      481 tgccgccatt tgtcgcgaat acaagcagac atggaaaacg ctggatga
//
LOCUS       DAZGOB010000517          523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZGOB010000517 DAZGOB010000000
VERSION     DAZGOB010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.520"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            228..>523
                     /locus_tag="ACHLIF_005645"
     CDS             228..>523
                     /locus_tag="ACHLIF_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO9609388.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPVSNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEEDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTG"
BASE COUNT          151 a          100 c          145 g          127 t
ORIGIN      
        1 gtgggtcctt tccggcgatc cgacaggtta cggggcggcg acctcgcggg ttttcgctat
       61 ttatgaaaat tttccgggat ccatgtccgg tttctcttca agttaactat atgaaaaata
      121 taaaaacagg tcttctgtga accggacatg aacaaaaaac agacatgtaa accggacatg
      181 accggttttg ttgtgattgt gaggtgagag tttttgcgag gtgaggagtg gctacgcaga
      241 ctgaagttgc caggcattta agtctgaccg atcgccagct tcgcagattg cagaaattac
      301 cgggtgcccc ggtctcgaat aagcgagggc aactggatct ggatgcctgg cgcgattttt
      361 acatatcgta tctgagaaga agtaaaaacg atgtgcctga tggcgatagc gaagaagact
      421 atgaagagaa attgcttatt gccagatggg aactgacagc agaacaggct gttacacagc
      481 agttaaaaaa tgaggtgtca aaaggaaaac tgattgacac cgg
//
LOCUS       DAZGOB010000518          520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZGOB010000518 DAZGOB010000000
VERSION     DAZGOB010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.521"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>520
                     /locus_tag="ACHLIF_005646"
     CDS             <1..>520
                     /locus_tag="ACHLIF_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9609389.1"
                     /translation="KKQCEEILKNCHTHINPKAIKYPLSFLLQNTGYTIAERAVVTFT
                     AKGDFRLCGFNWEIMEELTESKRIDLPSAPTAPKGKWRSVCGVTERMPYAGGALETPL
                     SSIISKEIGPLLSLTPTERDKNDFYYKEKRNEPVQVVSLECEEWRHQVKEEIFPLFVY
                     TANKPKKVNGAIE"
BASE COUNT          184 a           91 c          115 g          130 t
ORIGIN      
        1 aaaaagcagt gtgaagaaat actgaaaaac tgtcatactc atattaatcc gaaggcaata
       61 aaatacccgc tgtcgttttt attacaaaac acaggttata ctatcgctga acgtgctgtg
      121 gtaacattta cagcaaaagg agattttcgt ctgtgtggat ttaattggga gataatggaa
      181 gagctgactg aaagcaaaag gattgaccta ccttcagccc ctaccgcccc caaaggaaaa
      241 tggcgtagtg tatgtggcgt aacagaaaga atgccttacg ctgggggagc tttggaaaca
      301 ccgctatcca gtataatttc aaaagaaatt ggtcctttgt tatctcttac tccaacagaa
      361 agggataaaa acgattttta ttataaagaa aagagaaatg agccagttca agttgtatcg
      421 ctggaatgcg aagagtggcg tcatcaagtc aaagaagaaa tattcccgtt gttcgtatat
      481 acagccaaca aaccaaagaa agtgaatggt gcaattgagg
//
LOCUS       DAZGOB010000519          518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZGOB010000519 DAZGOB010000000
VERSION     DAZGOB010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.522"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..113
                     /locus_tag="ACHLIF_005647"
     CDS             <1..113
                     /locus_tag="ACHLIF_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609390.1"
                     /translation="DILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            94..>518
                     /locus_tag="ACHLIF_005648"
     CDS             94..>518
                     /locus_tag="ACHLIF_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO9609391.1"
                     /translation="MSQPVGDLIIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTATAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMLRGLAGAITLPMVGVTSLAVATGALAYAWYQG"
BASE COUNT           95 a          133 c          175 g          115 t
ORIGIN      
        1 gcgatatttt atcgtctgcg gatgtggcgg atgtcatggt ggatgatgcc gcattaatga
       61 tggcttcagc ggggattccg ggaggtgtga gatatgtccc agccggttgg tgatcttatt
      121 attgacctga gtctggatgc ggtccgtttc gatgagcaga tgagccgggt aaggcgtcat
      181 ttttcaggac tggatactga cgccagaaaa accgccactg ctgttgaaca gggcctgagc
      241 cgtcaggcgc tggctgcgca aaaagccggg atttccgtcg gacagtataa agcggccatg
      301 cgcaccctgc ccgcacagtt tacggatatc gccacgcagc ttgccggtgg tcagaatccc
      361 tggctgatcc tgctgcaaca gggcggtcag gtgaaggact ccttcggcgg gatgatcccc
      421 atgctcaggg ggcttgccgg tgcgatcacc ctgccgatgg tcggggtcac ctcgctggcg
      481 gtggcgacag gtgcgctggc gtacgcctgg taccaggg
//
LOCUS       DAZGOB010000520          512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZGOB010000520 DAZGOB010000000
VERSION     DAZGOB010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.523"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            243..>512
                     /locus_tag="ACHLIF_005649"
     CDS             243..>512
                     /locus_tag="ACHLIF_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609392.1"
                     /translation="MKVRSIGFTINNNNKNINTVDVMNAFINASNREHSRTDYTRKIL
                     ISDVNDFYYGLVVTFRNQKKNCKSQFVDGKFQLKIEDLQGSDKLAN"
BASE COUNT          181 a           97 c           90 g          144 t
ORIGIN      
        1 tgacatacca ataactcttg tctaaaaagc cagcaagata aaaagccagt attcacgacc
       61 accagcgtgt ttactgtact gcacaaggtt tacaggtaca aaaaaaccgc tcagcggcgg
      121 gtttaagttg tgtggcgaag taaccactct taacacgata taatactttt tgcgtacgcg
      181 ttaggatttt tataaactat gcgtccccat tctcgctatc tttagtcggt cctggaatac
      241 acatgaaagt tagaagcata ggatttacaa taaataacaa taacaaaaat attaataccg
      301 ttgacgtaat gaatgctttt atcaacgcat caaacagaga acacagtcgc acagactata
      361 ctcgtaaaat tctcatttcg gatgtgaatg atttctatta tggattagtt gtcacattca
      421 gaaaccaaaa aaagaactgt aagtcgcaat ttgttgatgg taaattccag cttaaaattg
      481 aagatcttca aggaagtgac aaacttgcta at
//
LOCUS       DAZGOB010000521          511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZGOB010000521 DAZGOB010000000
VERSION     DAZGOB010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.524"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..185
                     /gene="escF"
                     /locus_tag="ACHLIF_005650"
     CDS             <1..185
                     /gene="escF"
                     /locus_tag="ACHLIF_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO9609393.1"
                     /translation="GKTLSDSVPELLNSTDLVNDPEKMLELQFAVQQYSAYVNVESGM
                     LKTIKDLVSTISNRSF"
     gene            191..469
                     /locus_tag="ACHLIF_005651"
     CDS             191..469
                     /locus_tag="ACHLIF_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HFO9609394.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAESILNVLPQIIPDKKDI
                     AHLEFIILYGLNRKNDAVKALEDFMDDETSQLLCSLVHENNRSWTLMF"
BASE COUNT          165 a           79 c          107 g          160 t
ORIGIN      
        1 taggtaaaac gctgagtgat tctgtgccag agttacttaa tagcaccgat ttggttaatg
       61 accctgaaaa aatgttagag ctgcagttcg cggttcagca atattctgct tatgttaacg
      121 tagaaagtgg aatgctgaaa acgataaaag atttggtctc aaccatttcc aaccgtagct
      181 tttaattttt atggttaatg atgtttctgc caacaagata ctggtgtggg cggctgtagc
      241 tgcggcaaat cataagcttc ccaaatatgc agaatctatc ctgaatgtat tgccgcaaat
      301 tatacccgat aaaaaagata tcgcacattt agaatttatt attttatatg gattaaatag
      361 aaaaaatgat gcggtaaagg ctctggagga ctttatggat gatgagacaa gccagttgtt
      421 atgcagtctg gtccacgaga ataatagaag ctggacacta atgttttaat ctcatattga
      481 gtttaaacaa ttgataaatt tcattttggt a
//
LOCUS       DAZGOB010000522          501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZGOB010000522 DAZGOB010000000
VERSION     DAZGOB010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.525"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..372
                     /locus_tag="ACHLIF_005652"
     CDS             <1..372
                     /locus_tag="ACHLIF_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9609395.1"
                     /translation="IVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASM
                     ILGGVAQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRIS
                     QDISTRDEGGDGKVVVIGRQA"
BASE COUNT          118 a          117 c          160 g          106 t
ORIGIN      
        1 attgtgctgg gggcggcagc catcgtgggc tctttcttca ctgccggggc atcaatggcg
       61 ttatggggtt cagccctggc agccggtggt ttttctgcca ccacgatgct gttttcactg
      121 ggggccagca tgatactggg tggtgtggct cagatgctgg ccccgaaggc taaagtaccg
      181 gagtacaaaa gcacggataa cggcagacag aacacgtact tttcctcgct ggataacatg
      241 attgcacagg ggaacccgat gccggtgcct tacggtgaaa tgctggttgg ctcacggcga
      301 atctcccagg acatcagtac ccgtgatgaa ggcggtgacg ggaaggtggt ggttatcggg
      361 cggcaggcat aaaagcgaaa aaatcccgca gtgaccgaag gctgcgggaa cagaaaatga
      421 agattaacca cagggagttt tgtttttatt ggcccgaaaa aactgtaacg cccgggaatg
      481 atatctgcca cgggggcgta c
//
LOCUS       DAZGOB010000523          500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZGOB010000523 DAZGOB010000000
VERSION     DAZGOB010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.526"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..384)
                     /locus_tag="ACHLIF_005653"
     CDS             complement(<1..384)
                     /locus_tag="ACHLIF_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO9609396.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTYPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGI"
BASE COUNT          167 a           99 c           73 g          161 t
ORIGIN      
        1 aatgcctggc aatgaatact gccaaacttc atttttttct tttacagcaa atttgagacc
       61 taaaacttct atttgctgat tcaatgttaa cgttctatct tcaggttctt gtgctgttaa
      121 ttctctgaat gttactacca gatcagggta ggtagtaatt attatctcaa aactacctgt
      181 aagctcgcca taaggaacga ctattgtgtt ttctggtaac tcagaaaaag catcacttct
      241 tcctaacgaa acatcattac caatgataac aagtagacgc ccattaagta tttcatactc
      301 gcatccattc aatggtccgc tcaacaaacg aatggcataa gattccaata agtcttgtga
      361 taagaattta tcattatttt ccatgaatac cacccgctaa ttccataagc tctagtttag
      421 agtagtgtaa caagacggaa atttcatgac caaattatga tgacacagaa taaaaaaatt
      481 ataattccga gaactatttt
//
LOCUS       DAZGOB010000524          499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZGOB010000524 DAZGOB010000000
VERSION     DAZGOB010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.527"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>499
                     /gene="elaD"
                     /locus_tag="ACHLIF_005654"
     CDS             <1..>499
                     /gene="elaD"
                     /locus_tag="ACHLIF_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase ElaD"
                     /protein_id="HFO9609397.1"
                     /translation="LFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFHINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSE"
BASE COUNT          183 a          100 c           78 g          138 t
ORIGIN      
        1 cctgttcaaa aagctattct ccccttcgaa attaatgaca attatcggtg atgaaattcc
       61 tcttatatct gaaaaacagt cgctttcaaa ggtgctttta aatgatgaga ataatgaact
      121 gagtgatgat acaaacttct gggataaaaa tcgtcaatta accacagatg aaatagcttg
      181 ctatcttcag aagatcgccg ctaatgcaaa aaatactcaa gtcaattatc ctactggtct
      241 ctacgtcccc tactccacca gaactcacct ggaagacgct ctcaatgaaa atattaagag
      301 cgatccatca tggccgaatg aagtccagtt attccacata aatactggcg gacactggat
      361 attagtttcg ctacagaaaa tagtaaataa aaaaaataat aaactacaaa taaaatgcgt
      421 catattcaac tcattgcgtg cactaggcca tgataaagaa aattcactta agcgtgtcat
      481 taatagtttt aattctgaa
//
LOCUS       DAZGOB010000525          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZGOB010000525 DAZGOB010000000
VERSION     DAZGOB010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.528"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>485
                     /locus_tag="ACHLIF_005655"
     rRNA            <1..>485
                     /locus_tag="ACHLIF_005655"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAZGOB010000526          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZGOB010000526 DAZGOB010000000
VERSION     DAZGOB010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.529"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..293)
                     /locus_tag="ACHLIF_005656"
     CDS             complement(<1..293)
                     /locus_tag="ACHLIF_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO9609398.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVFYDVKTVL
                     EIDRERRQHNQRIPDDEGDLEERLLRARAELTEEQAVAQKLKNQVTEGKLIDTG"
BASE COUNT          139 a          126 c           73 g          147 t
ORIGIN      
        1 ccggtgtcaa taagcttacc ttcggttacc tgatttttaa gtttttgagc tacggcctgt
       61 tcttctgtca gttcagctct ggcccgaagc agcctttcct ccagatcgcc ctcgtcatca
      121 ggtattctct gattgtgttg tcgccgctcg cgatctatct ccagtacagt tttaacgtca
      181 taaaaaactt ccctcccccg acgctcaaca ggaggaacgc cccatttatc aaatgcctga
      241 acagatatac cgatggagga ggccatatca cttttattca ataaaaaggc catctcctct
      301 ccataagcca tcgataaaaa gcgatacaac aaccatgtgt ttttacaaaa ccatttgata
      361 tcattgacat ttttcgcatt gacgacatca aaacacatcg taaggttgtt gtatttattt
      421 tattttcacc ttacttatca attagatata ccaaacaatt aaacaacaac tccccctcaa
      481 aaaat
//
LOCUS       DAZGOB010000527          484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZGOB010000527 DAZGOB010000000
VERSION     DAZGOB010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.530"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>484
                     /locus_tag="ACHLIF_005657"
     CDS             <1..>484
                     /locus_tag="ACHLIF_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609399.1"
                     /translation="QIIKQKYFNDIVPMILALDIRNTYRPDNEMAFYYHIHSFLTQIP
                     DNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKDIFDGVYEFIRNIRKNSTPGKTKLI
                     ATINTCKETCKHLLYLSNEDKEKIISDLDKVQVACYYLTILLAFERRTSLTSILATLY
                     K"
BASE COUNT          179 a           90 c           73 g          142 t
ORIGIN      
        1 caaataataa aacaaaaata ttttaatgac attgtaccaa tgattctcgc actcgacatc
       61 aggaacacat atcgcccgga taatgagatg gcattttact atcatattca ttcttttctc
      121 actcagatac cggataatga ggatgatata tatcatgctg caaggacata tctgcgaaat
      181 tacgttaagt tatgtttatc cggatacacg ccagcgaatg cgcatttcaa agatatcttt
      241 gatggcgtat atgaattcat tcgtaatatt cgcaaaaaca gtacaccagg aaaaacaaaa
      301 cttatcgcaa ctatcaacac atgcaaagaa acctgtaaac atctgcttta tttaagtaat
      361 gaagacaagg aaaaaataat ttctgactta gataaagttc aggttgcatg ttattatctc
      421 actatattac tggctttcga aagacgaact tcattaacaa gcatcctggc aactttatat
      481 aaaa
//
LOCUS       DAZGOB010000528          482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZGOB010000528 DAZGOB010000000
VERSION     DAZGOB010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.531"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>482
                     /locus_tag="ACHLIF_005658"
     CDS             <1..>482
                     /locus_tag="ACHLIF_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9609400.1"
                     /translation="YRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARSAAQNAL
                     NSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDEIAH
                     KTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTGD
                     G"
BASE COUNT          115 a          124 c          165 g           78 t
ORIGIN      
        1 taccgtgctc agcaggccgt ggcggcagcc cgcgggacgg agatgcagat tgctgcagag
       61 gcccgtctgg cggccacaca ggaacgcctg aacagaaata ttgctgccag aagcgccgcc
      121 cagaatgcgc tgaacagtac aacggcggtg ggctcacgtc tgatgagcgg tgcgctgggg
      181 ctggttggtg gcgtacccgg actggtgatg ctgggggctg cagcatggta cacgctgtac
      241 cagaatcagg agcaggccag ggagtctgcg cgccagtatg cactgacgat agatgaaatc
      301 gcgcataaaa cgccgtcaat gtctttgcct gaagcctcag ataatgaagg acgaacacgg
      361 gcggcgctga cagagcagaa ccggctgatt gatgaacagg ccagtcgggt gaaatccctg
      421 caggaaaaaa tcgcaggata tcagtatgtt ctggcgaacc cgggctggac gaccggtgac
      481 gg
//
LOCUS       DAZGOB010000529          479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZGOB010000529 DAZGOB010000000
VERSION     DAZGOB010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.532"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>479
                     /locus_tag="ACHLIF_005659"
     CDS             <1..>479
                     /locus_tag="ACHLIF_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609401.1"
                     /translation="QGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAATRTLTLDREVT
                     LPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRL
                     FRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEV"
BASE COUNT           96 a          147 c          149 g           87 t
ORIGIN      
        1 caggggctgc ggcacacacc cggtgacatt attgaaatct gtgataatga ctatgccggg
       61 accctgaccg gcggacgtgt cctgtccatt gatgctgcca cccgcaccct gacgctggac
      121 cgtgaggtta ccctgccgga gacaggtaca tcggcggtga acctgattaa cggcagcggt
      181 aagccggtga gtgtggacat caccgcacac cccgcgccgg accggataca ggtcagtacc
      241 ctgcctgatg gtgtggagac atacggggtg tggggactct ccctgccgtc actgcgccgt
      301 cgcctgttcc gctgtgtctc cgtccgggaa aacacggacg gcacctttgc catcacggcg
      361 gtgcagcacg taccggaaaa agaagccatc gtggataacg gtgcccgctt tgagccgcag
      421 tcaggttccc tgaacagcgt catcccaccg gcagtacagc acctgacggt ggaggtgag
//
LOCUS       DAZGOB010000530          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZGOB010000530 DAZGOB010000000
VERSION     DAZGOB010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.533"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>476
                     /locus_tag="ACHLIF_005660"
     rRNA            <1..>476
                     /locus_tag="ACHLIF_005660"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZGOB010000531          475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZGOB010000531 DAZGOB010000000
VERSION     DAZGOB010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.534"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..155)
                     /locus_tag="ACHLIF_005661"
     CDS             complement(<1..155)
                     /locus_tag="ACHLIF_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO9609402.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTA"
     gene            complement(163..>475)
                     /gene="gpU"
                     /locus_tag="ACHLIF_005662"
     CDS             complement(163..>475)
                     /gene="gpU"
                     /locus_tag="ACHLIF_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO9609403.1"
                     /translation="AVFDEADFPAVAVYLTGAEYTGEELDSDTWQAELHIEVFLPAQV
                     PDSELDAWMESRIYPVMSDIPALAGLITTMVTQGYEYRRDDDMALWSSADLTYSITYE
                     M"
BASE COUNT          113 a          149 c          107 g          106 t
ORIGIN      
        1 gccgtcatct cgcccggcgt cagatccttc accttagcca gtcgctgcca gtcatcgtct
       61 gacaacgggt ttgcataagc atcacccttg ccgttgtaaa cccacagagt ggtaccggca
      121 ccttttaccg gctcaagggg atttggtgtt gccatatcgt cctcacatct cgtatgtaat
      181 ggaataagtc agatctgcag aactccataa cgccatatcg tcatcacgac gatactcata
      241 gccctgcgta accatcgtgg taatcagtcc tgccagtgcc gggatatcgc tcatcaccgg
      301 ataaatccgg gactccatcc acgcatccag ctctgaatcc ggcacctgag caggcaggaa
      361 aacttcaata tgcagctccg cctgccaggt atcgctgtcc agctcttcgc ccgtgtattc
      421 agcgccggtg agataaacgg caactgccgg aaaatccgcc tcatcaaaaa cagcg
//
LOCUS       DAZGOB010000532          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZGOB010000532 DAZGOB010000000
VERSION     DAZGOB010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.535"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          123 a          103 c          105 g          141 t
ORIGIN      
        1 gtttcggttt tttcaatcgc aaaggattag ataaatacat catggatttt atggatgtaa
       61 agaactttta ttagttatca agataatttg accctttgct agccgttccc ctatcatcat
      121 gatagtaagc cgtatgttta gatggtttat gtctgagatt ttgagtatca aatctttggg
      181 tctcgtaaaa aggtttgaat cgctaaggat aatctggacg tctccagggt cgttaattag
      241 actgacccca ccccgataga cgatcactcc cctatagtaa taagcaccat ttcggacttc
      301 cgcttctcgc tcacaacgga ccttcagctc agccgctccg gccgcttcat gcaatgaatg
      361 tacgcagcca gtcagaatat taaggtgcgg atttcagttc acttgtcagc cctgtgatgc
      421 aacaagttgc ggctccggtt cgtgggagag atgaaacgac ggtagctgaa gt
//
LOCUS       DAZGOB010000533          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZGOB010000533 DAZGOB010000000
VERSION     DAZGOB010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.536"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..446)
                     /locus_tag="ACHLIF_005663"
     CDS             complement(<1..446)
                     /locus_tag="ACHLIF_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9609404.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNG"
BASE COUNT          104 a          145 c          123 g           99 t
ORIGIN      
        1 ccgttaaagg cgtgcatggc cacaccttcc cgaatcatca tggtaaacgt gcgttttcgc
       61 tcaacgtcaa tgcagcaaca gtcatcttcg gcaaactctt tccatgccgc ttcaacctcg
      121 cgggaaaagg cacgggcttc ttcctccccg atgcccagat agcgccagct tgggcgatga
      181 ctgagtcgga aaaaagaccc gacgatatga tcctgatgca actggatggc gttggcggca
      241 tagccgttat tgcgtaccag atcgtctgcg cgggcattgc cacgggtaaa gttgggcagc
      301 agggctgcat ccacactttc acccggtggg ttccacgccc gcaactgccc accaaatccg
      361 ctgccaccgc cgtgataacc ggcatattca cgcagcgatg tcatgccgtc cggccccaga
      421 agggtgggaa tggtggacat tttcatacat aaaatcctgc tggtcccctg c
//
LOCUS       DAZGOB010000534          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZGOB010000534 DAZGOB010000000
VERSION     DAZGOB010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.537"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(304..>468)
                     /locus_tag="ACHLIF_005664"
     CDS             complement(304..>468)
                     /locus_tag="ACHLIF_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFO9609405.1"
                     /translation="YFISSEKKLTIHIVQMFHLLSQVFYNLKKFLMMDMLGVGDAINI
                     NANKNIRQVC"
BASE COUNT          145 a           95 c           71 g          157 t
ORIGIN      
        1 aggggaccat ttccaaagca ctgcaccacc acctggtgcc ggatgatctt gcttcagtgg
       61 taagagtacc tgatgcattg ttcccaaaat ggtgttttcc cctgcccctc caataatgtg
      121 tagctctcac ttgataatct attgattagg ttatattaag aatcaacatt taaaatacat
      181 ccattttctt gaataaatca acatgtttat taatcaattg ctgttttagc tctgactcat
      241 caagctctcc atatttgtcc tacagataat tgcgaataaa tacagagaac gccatttatc
      301 cttttagcat acctgccgga tatttttatt tgcattaata ttaattgcat ctccaactcc
      361 gagcatatcc atcattaaaa actttttgag attatagaat acctgtgata acaagtgaaa
      421 catctgcaca atgtgaatag ttagtttctt ttcagaggaa atgaaata
//
LOCUS       DAZGOB010000535          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZGOB010000535 DAZGOB010000000
VERSION     DAZGOB010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.538"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(177..404)
                     /locus_tag="ACHLIF_005665"
     CDS             complement(177..404)
                     /locus_tag="ACHLIF_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9609406.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
BASE COUNT          121 a           79 c          101 g          167 t
ORIGIN      
        1 ggaatctgtt caataaaaaa actgccgtgt tggagtcaca gcagtaatgt actgattggg
       61 tagaagatta ttattgttat gctttatttt tattctatat ggctgattat ttcaattcgg
      121 aattaataca gctaatgtct gtgatttttt ataaattcag caatataaag aaatagttat
      181 atgaacagcc atcgcagagc atactgtgta tcattctttt ttatagtcaa ctgacgggca
      241 tattttatgt ctgctgccag ctcccggcgg caagattcaa tgacccacgc agaaaaattt
      301 tctgaacctt tctggtcaag agcgatgtta atttgttcaa tcatctggtt tggaaatcgg
      361 atgttgcggg ttgttgttct gcggggccgg tttttcgatg acattttctt tcctctggtg
      421 acaagctata tggcgaggat tttacatggc tgtgcttcgt acgttacc
//
LOCUS       DAZGOB010000536          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZGOB010000536 DAZGOB010000000
VERSION     DAZGOB010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.539"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            106..>467
                     /locus_tag="ACHLIF_005666"
     CDS             106..>467
                     /locus_tag="ACHLIF_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFO9609407.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVL"
BASE COUNT          133 a          117 c          105 g          112 t
ORIGIN      
        1 caaccaaatt ctccccacat caacctcatt gtgtgggtat aattgcgggc ataccccagt
       61 tcgacagaat ttgtaccctc tttaacgcat tcagaaggct gaactatggc actgactgac
      121 gcaaaaatcc gggctgcaaa gcccactgac aaggcttata aactcactga cggggctggc
      181 atgttcctgc tggtacatcc taatggttcc cgttactggc gtctccgtta tcgtattctg
      241 ggtaaagaga agactctggc acttggtgtg tatccagaag tatctctctc cgaagctcgt
      301 acaaaacggg atgaggcccg aaaactgatt tcggaggggg ttgacccttg cgaacagaaa
      361 agagctaaaa aagtagtccc tgatttacaa ctctctttcg aacatattgc acgacgctgg
      421 catgccagta ataaacaatg ggcacaatca cacagcgata aagtact
//
LOCUS       DAZGOB010000537          463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZGOB010000537 DAZGOB010000000
VERSION     DAZGOB010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.540"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>463)
                     /locus_tag="ACHLIF_005667"
     CDS             complement(<1..>463)
                     /locus_tag="ACHLIF_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO9609408.1"
                     /translation="SIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGK
                     NNCPTMQLMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYPIKKAYTPYN
                     IHTYKTEEVVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFS"
BASE COUNT          135 a           69 c           85 g          174 t
ORIGIN      
        1 ggaaaacgat ctgtccagaa ggtctatcgg ttcctctctt attgcctgat tgtaataatc
       61 gttattacag taagtattgt ttatttcatc taaggtcatg tttttaactt ttatattcct
      121 ctggtttaca acttcctctg tcttgtaagt gtgtatatta tatggggtat atgctttttt
      181 aataggatag tgaaacaatt cgttatccac atctattccg tcagagtgaa cttcaaatgc
      241 accaggcgaa agaattgcca ggccagtatt aagcttaagt gctggggaaa acaaattaaa
      301 taacatgagc tgcatagtag ggcaattgtt cttcccagaa acatttaaaa aatttaagtt
      361 tttgctctgt tttattatgg ggaataaagt tccaatatcg gatgtattat tagtatcaat
      421 gtgggttatt ttattattgt ccagtcttaa acttttgata gat
//
LOCUS       DAZGOB010000538          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZGOB010000538 DAZGOB010000000
VERSION     DAZGOB010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.541"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            69..293
                     /locus_tag="ACHLIF_005668"
     CDS             69..293
                     /locus_tag="ACHLIF_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609409.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
BASE COUNT          122 a           98 c          122 g          115 t
ORIGIN      
        1 gtggaagggg cacaggacta tatccgcact cagtgtacta agcaggcttt ttattatccg
       61 gaggatgtat gaagaaatta caggtaacgg taaaaccttt tcagggaaca attccgttcc
      121 gtgttttgca acatggccgt gttctgcttg aagaggtgtt cagaggtaaa tgcactgaat
      181 gttattcacg aacatatgaa gtgaatgcca cgcatgaaga attcaccgtt gagtgtgtga
      241 tgaatactga taaatgccga atggtatccg ctgaattaca gccagtgtgt tgagcgacct
      301 tattatccat gcgcggtatt gtcgccgtat tcctgcatta acagagaccg cagcccgaca
      361 gggagactcc tctgcgagag tgtgcgggga taatcaaaaa cgatacacac cggggtttac
      421 cgcgtaaacg gagcgcggcg ttctcccctc atggtcg
//
LOCUS       DAZGOB010000539          454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZGOB010000539 DAZGOB010000000
VERSION     DAZGOB010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.542"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..108
                     /locus_tag="ACHLIF_005669"
     CDS             <1..108
                     /locus_tag="ACHLIF_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609410.1"
                     /translation="EDAIKMRKYEPYLLNDNSILSRIALLELGIIGEQQ"
     gene            105..293
                     /locus_tag="ACHLIF_005670"
     CDS             105..293
                     /locus_tag="ACHLIF_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO9609411.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            389..>454
                     /locus_tag="ACHLIF_005671"
     CDS             389..>454
                     /locus_tag="ACHLIF_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9609412.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAV"
BASE COUNT          150 a           97 c           95 g          112 t
ORIGIN      
        1 gaagatgcca ttaagatgag aaaatatgaa ccttatcttc tcaatgataa ttccatactt
       61 tccagaattg cccttcttga acttggtatt atcggagaac agcagtgact tcagcatttg
      121 cactggtgat gaccgttttt cttataacgg gtgagccaca aaatgtgatt accggaattt
      181 atgacagtaa gtcatcctgc attcaggtaa gggacgaaca aaaaatcccc ggtgaatgcc
      241 tcccgttaaa aaaagtatcg ctgaacctga ataacgaaat accggctgga taacccgcca
      301 gccatattaa cgccatacca acggattaaa aatgccagca atggcaggga ttcgttcacc
      361 ctgaaatctg taatgaggtt aaaacaaaat gagtaaagtc tttatttgcg ccgccattcc
      421 ggacgaacag gcaataaagg aagaaggtgc cgtc
//
LOCUS       DAZGOB010000540          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZGOB010000540 DAZGOB010000000
VERSION     DAZGOB010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.543"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>453
                     /locus_tag="ACHLIF_005672"
     CDS             <1..>453
                     /locus_tag="ACHLIF_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO9609413.1"
                     /translation="VRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          110 a          101 c          142 g          100 t
ORIGIN      
        1 gtgcgttaca cggcggcggt gcctgcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggctc cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtcct gatgaggaag agacgctgtt acgtgtggat
      181 gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgactat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgca tctgtctatg gcaagccggt gatcaccatg
      361 ccaaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZGOB010000541          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZGOB010000541 DAZGOB010000000
VERSION     DAZGOB010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.544"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>453
                     /locus_tag="ACHLIF_005673"
     CDS             <1..>453
                     /locus_tag="ACHLIF_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO9609414.1"
                     /translation="VRYTAAVPSRVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEGTLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          108 a          103 c          143 g           99 t
ORIGIN      
        1 gtgcgttaca cggctgcggt gccttcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggcac cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtccc gatgaggaag ggacgctgtt acgtgtggat
      181 gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgaccat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgcg tctgtctatg gcaagccggt gatcaccatg
      361 ccgaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZGOB010000542          451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZGOB010000542 DAZGOB010000000
VERSION     DAZGOB010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.545"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>451
                     /locus_tag="ACHLIF_005674"
     CDS             <1..>451
                     /locus_tag="ACHLIF_005674"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO9609415.1"
                     /translation="FSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMGGNHPLNRFTT
                     HMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKGGIAIGDGAV
                     IAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYS"
BASE COUNT          135 a           70 c           96 g          150 t
ORIGIN      
        1 atttctccag tgtcggcgct ttttcatttc cgacctgcca tttttcaggg aatatacgta
       61 taggtcgatt ttgtagtatt gcatcaaatg taaaaataat gggtggaaat catcctttga
      121 atcgttttac tactcacatg atgacttata acggtgaatt tgataagttt gctatgagtg
      181 aatttgaacg tagctggaca ttgaaaccat tcattaccaa acctgaaaat ccgattatag
      241 gaaatgatgt ctggatagga aatgatgttg tattgaaagg tggcatcgca ataggtgatg
      301 gtgctgttat tgctgccaat tccgttgtta ccaaagatgt gcccccatat gccatagttg
      361 caggagttcc tgcaaaaata atcagattta ggtttgattc aaatgttatt gatgaactgt
      421 tgagaataaa atggtggaat tataactact c
//
LOCUS       DAZGOB010000543          445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZGOB010000543 DAZGOB010000000
VERSION     DAZGOB010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.546"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>445)
                     /gene="tnpA"
                     /locus_tag="ACHLIF_005675"
     CDS             complement(<1..>445)
                     /gene="tnpA"
                     /locus_tag="ACHLIF_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO9609416.1"
                     /translation="RSYLPDALRLRFEDKLTIRAIAQRLGLSHSTIHTLFQRFIASGI
                     AWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSREFKVRLAKQALQP
                     GAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT"
BASE COUNT          111 a          108 c          111 g          115 t
ORIGIN      
        1 agtcagagcc accgggacag gcatgcattc ctgtatgtca tcatcgctca gtaagccgtc
       61 ctcgtactgg cttttccatt taaacagcag gttatcattg ataccgtgct ctctggcgat
      121 ccgggcaaca acagcaccgg gctgtaatgc ctgcttagcc agacggacct taaattcacg
      181 gctgtagctg gctcgccgtt cttttcgcca tgtgccttcg ctgatttgag gctctgttaa
      241 ttccttcttt ctgttggcat aaaggatggc atcaagctga gctaatgaaa ctgaatcggg
      301 caatggccat gcgataccgg atgcaataaa tcgctgaaaa agcgtatgta ttgtggaatg
      361 actgagacct agacgctgag cgatggcccg gatggtcagt ttatcttcaa atcttaaacg
      421 cagagcatca ggcaaataag aacgg
//
LOCUS       DAZGOB010000544          442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZGOB010000544 DAZGOB010000000
VERSION     DAZGOB010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.547"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>442
                     /locus_tag="ACHLIF_005676"
     CDS             <1..>442
                     /locus_tag="ACHLIF_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9609417.1"
                     /translation="VRLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEANKPKSRG
                     GKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTAQGELKTLTEMQKQELLRN
                     AALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERAR"
BASE COUNT          127 a           96 c          150 g           69 t
ORIGIN      
        1 gtccgactgg ggtatgcggc ggatgacctc ggttttgtgg gtgatccgta tcaggaggcg
       61 agacaacgtt atatcagtaa tgccctggaa gcctggcgca ataacgaggc gaataaaccc
      121 aaatcccggg gtggaaaatc agagacggaa aaagcggaag acagtttttc ccggctgctg
      181 aagcagcaga aagagcaact ggcactggtg gggcagaata cagagctggc gaagctgaaa
      241 taccagactg cgcagggcga actgaaaacc ctgacggaga tgcagaagca ggagctgctg
      301 cgtaatgcgg ccctgattga ccagcaaaaa atccgggaac agttgcgatc ccgggaagag
      361 acactgaaga atgagaatgc ggctgcgcgt gcgtcgaatg atgctgaact gctggggtac
      421 gggcaggggg aacgagccag ag
//
LOCUS       DAZGOB010000545          439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZGOB010000545 DAZGOB010000000
VERSION     DAZGOB010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.548"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(84..380)
                     /locus_tag="ACHLIF_005677"
                     /pseudo
     CDS             complement(84..380)
                     /locus_tag="ACHLIF_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          101 a          116 c           89 g          133 t
ORIGIN      
        1 actttggttc ccactactta tttgttggac accactttgt ctaattcgtc agattctgac
       61 cagacggttc aggctgtacg cttacgatta cgctgaactt cacgtgagat cgtcgaagga
      121 ctgcgattca gcgcagtagc tatcgcacga atgctcattt tggctgacaa accagctcgt
      181 atctcctcgc gctcagacag tgtcaggtga gctacagccc gcttacgctc atggggtttt
      241 atgccgccag tatcccttaa catagtgaag atcgttccgg gttttgaacc caggatattc
      301 gctatttcac tgaagcctgt tccgttcttc catagttcaa aaacagaggc tttttcctct
      361 gctgtaaatg ttcgtctcat tcaaaaaacc tccgcaaccc catgttttca cataactgtt
      421 gcgttgacca attgaatct
//
LOCUS       DAZGOB010000546          434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZGOB010000546 DAZGOB010000000
VERSION     DAZGOB010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.549"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>434)
                     /locus_tag="ACHLIF_005678"
     CDS             complement(<1..>434)
                     /locus_tag="ACHLIF_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO9609418.1"
                     /translation="LIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVYTTTQSNEPP
                     AGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNLGYSV
                     DEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAG"
BASE COUNT           99 a          135 c          108 g           92 t
ORIGIN      
        1 cccgcccagc ggtcagaacg cagggcaaga ccgatttcca cattggcgtg ttttgacatg
       61 aagccacgaa atgcttcctc accagcctcc cgggctttac ggtactcccg gtacagaaag
      121 gcctcatcca ccgaataacc gagattcggg ttaaccatgg cgaggttttc catcagcagg
      181 tgagccccgc tttccaccat ttcaggagga tgctcaaaaa tcaccggcag aaagtgcgga
      241 tcatgaattt tgccgtcacg gacatcccgg gcatactgca gtttctgtct gaacaccccg
      301 gcgggcggtt cattcgactg ggtggtcgta tacaccacaa acccttccgg acgggaggcc
      361 agcccgccga tggcttcacg tagcatgtct tccgccttgt actgcttgcc aaacagccac
      421 agttcatcaa tcag
//
LOCUS       DAZGOB010000547          432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZGOB010000547 DAZGOB010000000
VERSION     DAZGOB010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.550"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>432
                     /locus_tag="ACHLIF_005679"
     CDS             <1..>432
                     /locus_tag="ACHLIF_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO9609419.1"
                     /translation="NYIADDIEQVIDKGSKVQLEVYNLLSEEKIFEHKIMNNWTRSIK
                     NILTTYLFMSSGAVTARNVQTFSPTINNESRIRLPRALPVGHPYPEEHKASGFSPFMM
                     GGLSGDILPEILTGNGPSIFFNGKHNNQHDGAFGKIIDFTQ"
BASE COUNT          156 a           64 c           88 g          124 t
ORIGIN      
        1 taactatatt gcagatgata tagagcaagt aatcgataaa ggaagcaaag ttcaattgga
       61 ggtatataat ttattatccg aagaaaagat atttgaacat aaaattatga ataattggac
      121 aaggagcata aaaaatatat tgacgacata tttgtttatg tcatcaggag cggtgacagc
      181 cagaaatgtt caaacctttt ctccaacaat aaataatgag tcaaggattc gattgccgag
      241 agcattgcca gtaggccatc catatcctga ggaacataag gcttctggtt tctccccttt
      301 tatgatgggg gggctgagtg gtgatattct tccggaaatt ttaacgggga atggaccatc
      361 tatatttttt aacggaaaac ataataacca acatgatgga gcttttggaa aaataataga
      421 ttttacccaa aa
//
LOCUS       DAZGOB010000548          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZGOB010000548 DAZGOB010000000
VERSION     DAZGOB010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.551"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            47..>430
                     /locus_tag="ACHLIF_005680"
     CDS             47..>430
                     /locus_tag="ACHLIF_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000479108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9609420.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTESPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHVDTDITEVAKNNA"
BASE COUNT          103 a          104 c          134 g           89 t
ORIGIN      
        1 gtcaccgtta ctgaagcggg cgctgcaggg taaacggagg taatacatgt ttctgaaaac
       61 cgaacaattt gaatataacg gtgtgtccgt cacgctttcc gagctgtctg cgctgcagcg
      121 tatcgagcat cttgccctgc tgaaacgacg ggcagaacag gctgaagcca gcggtaacct
      181 gcaggtgagc gtggaagacc ttgtcagaac cggcgcgttt ctggtggcga tgtccctgtg
      241 gcataaccat ccacagaaaa cggagtcacc atcaatgaat gaggctgtga tgcagatcga
      301 acaggaggtg ctcaccacct ggcctgctga tgccattgcc cgggcggaag acgtggtgtt
      361 gcgtctgtcc gggatgagcg gggctgttca tgtggatacg gatatcaccg aagtggcgaa
      421 aaataacgcg
//
LOCUS       DAZGOB010000549          424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZGOB010000549 DAZGOB010000000
VERSION     DAZGOB010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.552"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..415
                     /locus_tag="ACHLIF_005681"
     CDS             <1..415
                     /locus_tag="ACHLIF_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO9609421.1"
                     /translation="LAARKARAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWD
                     ALNVYCSNGWVEIDNNIAENALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNN
                     VEPEKWLRYVIEHIQDWPANRVRDLLPWKVDLSSQ"
BASE COUNT          115 a          100 c          125 g           84 t
ORIGIN      
        1 tctggcggca agaaaagcca gagccgcgcc actgatgcag tcactgtatg actggataca
       61 gcaacagatg aaaacactgt cgcgtcactc agatacggca aaagcgttcg catacctgct
      121 gaaacagtgg gatgcactga acgtgtactg cagtaatggc tgggtggaaa tcgacaacaa
      181 catcgcagag aacgccttac ggggagtggc cgtaggccgg aaaaactgga tgttcgcggg
      241 ttccgacagc ggtggtgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct
      301 gaacaatgtg gagccagaaa agtggctgcg ttacgtcatt gaacatatcc aggactggcc
      361 ggcaaaccgg gtacgcgatc tgttgccctg gaaagttgat ctgagctctc agtaaatatc
      421 aata
//
LOCUS       DAZGOB010000550          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZGOB010000550 DAZGOB010000000
VERSION     DAZGOB010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.553"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>412)
                     /locus_tag="ACHLIF_005682"
     CDS             complement(<1..>412)
                     /locus_tag="ACHLIF_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9609422.1"
                     /translation="WRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASAST
                     GTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGY
                     AEGGYVGGAGSSAQMRRTEGINFNQNNHVVIQNDG"
BASE COUNT           92 a          144 c           98 g           78 t
ORIGIN      
        1 gccgtcgttc tgaatcacca cgtgattgtt ctgattaaaa ttaatgcctt ccgtccgccg
       61 catctgcgcc gaacttccgg caccgcccac ataaccacct tccgcatagc cccgcatcag
      121 gcggtacagg ttgccgacgc caatccggct ggttgcttcc ttcgtgaaga caaactcccc
      181 gcgatggaca ataccggcag gttcgtattt gccaccggtt cccgtaaatc ctccggtcgc
      241 gaaatggaag ttcgccgccg cagcctgaat ggccgtcccc gtggaggcag acgcaccacc
      301 accgaaagca ccgccaatgg cgctgccgat actcccgact atccccacca tcgcctgctt
      361 cagaaaaatc tctgtcagca tggagagcac agaacgggtg aaaccacgcc ag
//
LOCUS       DAZGOB010000551          407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZGOB010000551 DAZGOB010000000
VERSION     DAZGOB010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.554"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            207..326
                     /locus_tag="ACHLIF_005683"
                     /pseudo
     CDS             207..326
                     /locus_tag="ACHLIF_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          103 a          110 c           67 g          127 t
ORIGIN      
        1 tcacatagga tcagatctcg tctgatcgtt ccccctgcgc tcactaccat ggctctttac
       61 cacagcagcc ggggttgttt gggaccgact tccgacagca gatcccggtg ggcctgccac
      121 catctcttca caagcttatg cttattacat gcaaaacgtc ccgttttgca tctttgtgcc
      181 tacggcatcc ctgtgatctt tccctgaatc tgcccctgag aacctgaatc aaacgtctga
      241 ctcgcagaac catctgtttc tcgcgctctg tagaacttca tgaaaaagtc atcggtgctt
      301 ttatcgaaaa atacatgttc tactaattga agtcatcatc aaaaaattta attaatcgaa
      361 tcatacctat aaccttttaa ttttactttt ttgtcttcaa cattaaa
//
LOCUS       DAZGOB010000552          406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZGOB010000552 DAZGOB010000000
VERSION     DAZGOB010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.555"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..324
                     /locus_tag="ACHLIF_005684"
     CDS             <1..324
                     /locus_tag="ACHLIF_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609423.1"
                     /translation="MVSIRLPFISIYWLNREATNYWYTCARAAFNDPDWFVKNHHAVR
                     QAKRKANMTYMKAYQKAWKEHRDRYQQDMEKLESENMELRRKLGEAKRDIDAYKRLFN
                     GESHA"
     gene            284..>406
                     /locus_tag="ACHLIF_005685"
     CDS             284..>406
                     /locus_tag="ACHLIF_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO9609424.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHV"
BASE COUNT          140 a           86 c           94 g           86 t
ORIGIN      
        1 atggtgtcta tccggttgcc atttatcagt atttattggc taaacaggga ggcaacaaat
       61 tattggtata catgcgccag agcagcattt aacgaccctg actggtttgt gaaaaaccac
      121 cacgcagttc gtcaggcaaa gagaaaggcc aatatgacat acatgaaggc gtatcaaaaa
      181 gcatggaaag aacaccgcga tcgataccaa caagacatgg aaaagcttga atcagaaaac
      241 atggaattaa gacgaaagct cggtgaagca aaacgagaca ttgatgctta caagcgactt
      301 tttaatggtg aaagccatgc ttagcccatc ccaatccctt caataccaga aagaaagcgt
      361 cgagcgagct ttaacgtgcg ctaactgcgg tcagaagctg catgtg
//
LOCUS       DAZGOB010000553          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZGOB010000553 DAZGOB010000000
VERSION     DAZGOB010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.556"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>404)
                     /locus_tag="ACHLIF_005686"
     CDS             complement(<1..>404)
                     /locus_tag="ACHLIF_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609425.1"
                     /translation="DLTGVNQVSFRHLRESSSTRNQLNKILRQEIQDKISGVELSDIN
                     KEGFLYQSIGKIRLLALSSALFEIQCPDYIFSRLYRETLIREIGYQNVKQLSFYWQGG
                     QCKPEYGEERFCSELIKYGAGNLEWLFSDNPL"
BASE COUNT          129 a           84 c           55 g          136 t
ORIGIN      
        1 ataagggatt atcagaaaat agccattcaa gatttcctgc accgtatttt atcagttcag
       61 aacaaaaccg ttcttcgcca tattctggtt tacattgccc tccctgccaa tagaatgata
      121 actgcttcac attttgatat cctatctctc tgatcagagt ttctcgatat aatcgtgaaa
      181 aaatatagtc agggcattga atttcaaata atgcactact tagagcaagc aatcttattt
      241 tcccaattga ctgatataaa aaaccttcct tatttatatc agataattcc actccagaaa
      301 ttttatcttg tatttcctga cgcaatatct tgttgagttg attacgagta gaactacttt
      361 ctcttaaatg ccgaaaacta acctgattga caccggttaa atca
//
LOCUS       DAZGOB010000554          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZGOB010000554 DAZGOB010000000
VERSION     DAZGOB010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.557"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>404
                     /gene="espX1"
                     /locus_tag="ACHLIF_005687"
     CDS             <1..>404
                     /gene="espX1"
                     /locus_tag="ACHLIF_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO9609426.1"
                     /translation="LCFESDLSNTIFSNGEINNLFIKKSNIFGASFTNTRIKNLLCED
                     IMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVY
                     KELNKFMHDYDKTSSEMRKYLKSINECMFLMK"
BASE COUNT          155 a           70 c           63 g          116 t
ORIGIN      
        1 ttatgttttg aaagcgactt atcaaacacc atcttttcaa atggagaaat taataatctc
       61 ttcattaaaa aaagcaacat atttggagca tcattcacta ataccagaat aaaaaatttg
      121 ctctgtgaag atattatgcc tggtcgatgg accactcaac ttgtcaataa gcacttaggt
      181 tacagataca caggagtatt caaaaccctg gcaagtattg atgacaaacc gtcccgtttc
      241 gaaattttga taccattggt acaaacttta gttcgcgaca atgtaaagct aaataatgat
      301 gtgtataaag aacttaataa gtttatgcac gattacgaca aaacatcatc agaaatgaga
      361 aaatacctta aaagcatcaa cgagtgtatg ttccttatga aaaa
//
LOCUS       DAZGOB010000555          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZGOB010000555 DAZGOB010000000
VERSION     DAZGOB010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.558"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>403
                     /locus_tag="ACHLIF_005688"
     CDS             <1..>403
                     /locus_tag="ACHLIF_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609427.1"
                     /translation="TGGRILSIDAASRTLTLDREVTLPETGTSTVNLINGSGKPVSVD
                     ITAHPAPDRIQVSTLPDGVATYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHV
                     PEKEAIVDNGARFEPMSGSLNSVIPPAVQHLT"
BASE COUNT           78 a          128 c          126 g           71 t
ORIGIN      
        1 tgaccggcgg acgtatcctg tccatcgatg ccgccagccg caccctgacg ctggaccgtg
       61 aggtgacact gccggagaca ggtacatcga cggtgaacct gattaacggc agcggtaagc
      121 cggtgagtgt ggacatcacc gcacaccccg cgccggaccg gatacaggtc agtaccctgc
      181 cggatggcgt ggcgacatac ggtgtgtggg gactctccct gccgtcactg cgtcgtcgcc
      241 tgttccgctg tgtttccatc cgggaaaaca cggacggcac ctttgccatc acggcggtgc
      301 agcatgtgcc ggaaaaagaa gccattgtgg ataacggggc ccgctttgag ccgatgtccg
      361 gctcactgaa cagcgtcatc ccgccggcag tgcagcacct gac
//
LOCUS       DAZGOB010000556          400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZGOB010000556 DAZGOB010000000
VERSION     DAZGOB010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.559"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..366
                     /locus_tag="ACHLIF_005689"
                     /pseudo
     CDS             13..366
                     /locus_tag="ACHLIF_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310463.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          132 a           59 c           80 g          129 t
ORIGIN      
        1 atgaggtggg agatgttttc tacaaactta atacagagta attatggtga tttaaatatt
       61 aaaactctgg cttttgactc ttttaaagaa aggcttgagt ctaccatggc ggcactcact
      121 ttctttatat caacgggtca atgtgactgc gatgaaatag cagaatcaaa cttcaatcgt
      181 atgattgcct atatgtctaa cattaactat gatgcctcaa agccaggagc gccagcactt
      241 agttttgata catatctgca ggacaatgtt aaatatagag taataattaa taatctttat
      301 ggcggtgagt ttcggataag agatattaat gagaattttg ttgggatgga ttttacctgt
      361 gaattctgac ccgaaaaaat gacgaatttg atcaatataa
//
LOCUS       DAZGOB010000557          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZGOB010000557 DAZGOB010000000
VERSION     DAZGOB010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.560"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>399
                     /locus_tag="ACHLIF_005690"
     CDS             <1..>399
                     /locus_tag="ACHLIF_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9609428.1"
                     /translation="TGLKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRI
                     VRALAAGMGVARKDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKV
                     ENAFMAWVGGANEASGVTKTLSGMLNGVAGQ"
BASE COUNT           93 a           86 c          141 g           79 t
ORIGIN      
        1 acagggctga aaatctccgg tgccagtacg gcagaggcgg gctcggtgat cacccagttc
       61 agtcaggcgc tggcgcaggg tgtattgcgt ggtgaggagt ttaattcggt caatgaaagt
      121 ggtgaccgga tcgtacgtgc actggctgcg ggtatgggcg tggcccgtaa agatctgaag
      181 gcaatggcgg atgacggaaa actgacagcg gataaagtgg tccccgcgtt aatcagccag
      241 ctggggatat tacgtgatga atatgcggcc atgccggaaa cggtttccag tagtatcaca
      301 aaggtggaaa acgcctttat ggcctgggtg ggcggtgcga atgaggccag cggggtgaca
      361 aaaacgctct ccggcatgct gaacggtgtt gccggacag
//
LOCUS       DAZGOB010000558          388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZGOB010000558 DAZGOB010000000
VERSION     DAZGOB010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.561"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          156 a           69 c           54 g          109 t
ORIGIN      
        1 tagtattagt gacctgagac agagcattag cgcaaggtga ttttatgtct tcttgcgcta
       61 attttttgtc ataaaaacat gtggcagact ttgtatatct ctgctttgcc acgccacacc
      121 atcatgcatt atggctatgc ctaaacagaa tcaataagaa tagaacataa aaaatgacaa
      181 ctctactaga caattaacta caaaaatgat agcaattagt ttatataata taagacttcc
      241 tatcaaaagt tcatccatat gaagacaagg aatttattat gccaatcata aagaactgct
      301 tatcatcaat tagtaacata ttacgcaacg aaaaaacatc atattctcta ataaaaacag
      361 agcaaacagg aaaactgtta aacagaaa
//
LOCUS       DAZGOB010000559          387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZGOB010000559 DAZGOB010000000
VERSION     DAZGOB010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.562"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>387
                     /locus_tag="ACHLIF_005691"
     CDS             <1..>387
                     /locus_tag="ACHLIF_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFO9609429.1"
                     /translation="VRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDV
                     IDIKFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKTLLNGKYFDSGYIPCSRA
                     DESIQFSYADMESFSYSTSPEGKQYIE"
BASE COUNT          121 a           67 c           67 g          132 t
ORIGIN      
        1 gttcgttttg ctctcagttt ccgttacccc ggctgggcaa aaatgatgat tattaatggt
       61 gttactttta ctaaaaatga tactcaaaac tcacttatta ttttagatag aacatggcaa
      121 catcatgatg tgattgacat taaatttata gctgatatac agtttaatac tgacttatgt
      181 ggtgacactt atatttccag aggtcccgtt ttatatgcaa ttgagcttga gagcgatatt
      241 ttaatcaaaa aaactctgct gaacgggaaa tattttgact caggatacat accctgtagt
      301 agagctgatg aatctatcca gttctcttat gctgatatgg aatctttttc atattcaacc
      361 tcaccagagg gcaaacaata cattgaa
//
LOCUS       DAZGOB010000560          384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZGOB010000560 DAZGOB010000000
VERSION     DAZGOB010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.563"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>384)
                     /locus_tag="ACHLIF_005692"
     CDS             complement(<1..>384)
                     /locus_tag="ACHLIF_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9609430.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFSNGRGFWCLGGKA"
BASE COUNT           89 a          108 c           98 g           89 t
ORIGIN      
        1 ccgctttacc gcccaggcac cagaagccac gaccattgga aaaacgcttc atagtgagcg
       61 tgttatcccg gtgctttttg ccataccacg gagccagcgc cagcagcgac ggaatatcgc
      121 ggatggtcgg ctcaacgtgg gttttcataa agttctcggc atcaccatcc gtcggcaacc
      181 agataagtgt gttgcgctgc ttatgctcta tgaagtaggc ataaacaccc agcagcattt
      241 tggaataacc aacacgggca gacttcacca cattcacctc gcggatgtag tcgctgccca
      301 tcgcattcat gatggcccgc tgaaagggca gtgtttccca gcgcccttcc tggtatgcgg
      361 attctttcgg gagatagtaa ttgg
//
LOCUS       DAZGOB010000561          380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZGOB010000561 DAZGOB010000000
VERSION     DAZGOB010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.564"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            90..>380
                     /gene="grlR"
                     /locus_tag="ACHLIF_005693"
     CDS             90..>380
                     /gene="grlR"
                     /locus_tag="ACHLIF_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO9609431.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKVLSNDSNVTLHCHVRGNEK"
BASE COUNT          131 a           51 c           86 g          112 t
ORIGIN      
        1 gagaaaaaga aaggtctccg ttattcttga tatcgcttat ggatagaaca aattgaaagg
       61 agtgaggtta gtatgaaact gagtgagtta tgattatgaa ggatggcatc tatagcatta
      121 tctttattag caatgaagat tcctgtgggg aaggtatact gattaaaaat ggaaatttga
      181 tcactggcgg agatattgct tctgtgtatc agggggtcct gtctgaagag gaggacatca
      241 tacttcatgt ccatcgctat aatcatgaaa ttccctcggt gctaaacatt gaacaagatt
      301 atcaattagt tatccctaaa aaagtactga gtaatgatag taatgtcaca ttacattgcc
      361 atgtaagagg aaatgaaaaa
//
LOCUS       DAZGOB010000562          377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZGOB010000562 DAZGOB010000000
VERSION     DAZGOB010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.565"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>377)
                     /locus_tag="ACHLIF_005694"
     CDS             complement(<1..>377)
                     /locus_tag="ACHLIF_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO9609432.1"
                     /translation="DVISKVSFLSSNLQHVTFECKEIGDCNFTTAIVDNVIFRCRRLH
                     NVIFIKASGECVDFSKNILDTVDFSQSQLTQSNFRECQIRNSNFDNCYLYASHFTRAE
                     FLSAKEVSFIKSNMTAVMFDHVR"
BASE COUNT          131 a           69 c           60 g          117 t
ORIGIN      
        1 ttcgcacatg atcaaacata acagctgtca tattcgattt aataaatgat acttctttgg
       61 cagacagaaa ctctgctctg gtgaagtgcg aagcgtaaag ataacaatta tcgaagtttg
      121 aatttctaat ctgacattcg cgaaaattac tctgagtaag ttgactctgc gagaagtcaa
      181 ctgtatcaag aatatttttg ctgaaatcga cacattcacc actcgctttg ataaaaatca
      241 cattgtgtaa acgtcgacat ctaaatatga cattatcaac tattgcagta gtaaaattgc
      301 aatccccaat ttctttacat tcgaatgtta catgttgtag atttgatgat aaaaaagaca
      361 ccttcgatat aacgtca
//
LOCUS       DAZGOB010000563          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZGOB010000563 DAZGOB010000000
VERSION     DAZGOB010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.566"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>368
                     /locus_tag="ACHLIF_005695"
     CDS             <1..>368
                     /locus_tag="ACHLIF_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFO9609433.1"
                     /translation="SPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSSENSGESS
                     DQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIR
                     LMRERDGRNHRDMCVLFRWA"
BASE COUNT          114 a           87 c           94 g           73 t
ORIGIN      
        1 tcccctaaaa cgagggataa aacatccctc aaattagggg attgctatcc ctcaaaacag
       61 ggggacacaa aagacactat tacaaaagaa aaaagaaaag attattcgtc cgagaattct
      121 ggcgaatcct ctgaccagcc agaaaacgat ctttctgtgg ttaaaccgga tgctgcaatt
      181 cagagcggca gcaagtgggg aacagcagaa gacctgaccg ccgcagagtg gatgtttgac
      241 atggtgaaga ccatcgcacc atcagccaga aaaccgaatt ttgcagggtg ggctaacgat
      301 atccgcctga tgcgtgaacg tgacggacgt aaccaccgcg atatgtgtgt gcttttccgc
      361 tgggcctg
//
LOCUS       DAZGOB010000564          367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZGOB010000564 DAZGOB010000000
VERSION     DAZGOB010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.567"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            31..>367
                     /locus_tag="ACHLIF_005696"
     CDS             31..>367
                     /locus_tag="ACHLIF_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9609434.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSY"
BASE COUNT           97 a          108 c           91 g           71 t
ORIGIN      
        1 taaatcttaa ccaccgataa ggagcaaatt atgcgactag ccagtcgttt tggttatgcg
       61 aaccagatac gccgtgaccg tccgctgaca cacgaagaac tgatgcacta tgtgccgggg
      121 attttcgggg aagataaaca cacgtcccga agccagaact atacgtacat ccccaccatc
      181 accgtactgg aaagcctgca gcgggaaggc tttcagccat tcttcgcctg ccagacccgt
      241 gtgcgcgacc cgggccgccg gggatacaca aaacacatgc tgcgtctgcg gcgggccgga
      301 gagataaacg gagaacatgt ccctgaaatt attctgctca actctcatga cggtacctcc
      361 agctacc
//
LOCUS       DAZGOB010000565          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZGOB010000565 DAZGOB010000000
VERSION     DAZGOB010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.568"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..311
                     /locus_tag="ACHLIF_005697"
     CDS             <1..311
                     /locus_tag="ACHLIF_005697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609435.1"
                     /translation="IRAGVQYYDRQTKKKEWTNYSAVVFAKPGAQADYYRSVLVEGGI
                     VEITGENIRVDVYQGQNGQSITLELLNAKIGFAASGNGPQQQSSNQQNTPVYDDSIPF
                     "
BASE COUNT          112 a           75 c           90 g           89 t
ORIGIN      
        1 acatccgtgc tggcgttcag tattacgatc gccagacaaa aaagaaagaa tggacaaact
       61 acagcgccgt tgtatttgcc aagccgggag cgcaagcgga ttactaccgt agtgttcttg
      121 ttgaaggtgg cattgtggaa attaccggag aaaacatcag ggttgatgtt tatcaggggc
      181 aaaatggtca atcaatcact cttgaattac tgaatgcaaa gattggattt gcagcttcag
      241 gaaatggccc gcagcagcaa agtagtaacc agcagaacac tcctgtatac gacgattcca
      301 tcccattctg atttagaaaa ataaggattt aattatgcca gcgcctctgt atggtgcgga
      361 tgacgc
//
LOCUS       DAZGOB010000566          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZGOB010000566 DAZGOB010000000
VERSION     DAZGOB010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.569"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            169..>360
                     /locus_tag="ACHLIF_005698"
     CDS             169..>360
                     /locus_tag="ACHLIF_005698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9609436.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGT"
BASE COUNT           90 a           89 c           89 g           92 t
ORIGIN      
        1 aaaatcggcc agttttctct gcctgcagtc cgcatgccgt atcgggcctt gggttctaac
       61 ctgttgcgta gatttatgca gcggactgcc tttctcccaa agtgataaac cggacagtat
      121 catggaccgg ttttcccggt aatccgtatt tgcaaggttg gtttcactat ggaacatgaa
      181 cttcattata tcggtatcga caccgctaaa gagaaactgg atgtcgatgt gttgcgtcct
      241 gatggtcgtc atcgcaccaa aaaattcgct aacaccacta aagggcacga tgagctggtg
      301 agctggctga aaggtcacaa gattgaccat gcgcatatct gcatcgaagc gaccggcacc
//
LOCUS       DAZGOB010000567          358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZGOB010000567 DAZGOB010000000
VERSION     DAZGOB010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.570"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>358
                     /locus_tag="ACHLIF_005699"
     rRNA            <1..>358
                     /locus_tag="ACHLIF_005699"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          109 a           81 c          112 g           56 t
ORIGIN      
        1 cataggttaa tgaggcgaac cgggggaact gaaacatcta agtaccccga ggaaaagaaa
       61 tcaaccgaga ttcccccagt agcggcgagc gaacggggag cagcccagag cctgaatcag
      121 tatgtgtgtt agtggaagcg tctggaaagg cgcgcgatac agggtgacag ccccgtacac
      181 aaaaatgcac atgctgtgag ctcgatgagt agggcgggac acgtggtatc ctgtctgaat
      241 atggggggac catcctccaa ggctaaatac tcctgactga ccgatagtga accagtaccg
      301 tgagggaaag gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga aaccgtgt
//
LOCUS       DAZGOB010000568          354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZGOB010000568 DAZGOB010000000
VERSION     DAZGOB010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.571"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>354
                     /locus_tag="ACHLIF_005700"
     CDS             <1..>354
                     /locus_tag="ACHLIF_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609437.1"
                     /translation="YKFINCQEHETNNIHSAMINNLLQEVDIALGKINDIIDSRNISA
                     PHELANILTREKILTTREKKGNLISLFDGFTLFHCVGMITFLIHYLRTPEEKVENIFM
                     LYGADKNNKLRRRLI"
BASE COUNT          140 a           63 c           54 g           97 t
ORIGIN      
        1 tttataagtt cataaactgt caggaacatg aaaccaataa cattcactca gctatgataa
       61 ataacctatt acaggaagtt gatatagccc ttggtaaaat aaatgacatt atagacagca
      121 gaaacatatc agccccccat gaactggcaa atattcttac ccgcgaaaaa atacttacaa
      181 cacgggaaaa aaaaggaaac ctgataagcc tgtttgatgg attcacttta ttccattgtg
      241 ttggaatgat aacctttctt atccattatc tcagaacacc tgaagaaaaa gttgaaaata
      301 tatttatgtt atatggtgca gataaaaaca ataaactacg cagaagactg attt
//
LOCUS       DAZGOB010000569          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZGOB010000569 DAZGOB010000000
VERSION     DAZGOB010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.572"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..102
                     /locus_tag="ACHLIF_005701"
     CDS             <1..102
                     /locus_tag="ACHLIF_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609438.1"
                     /translation="KSAKGDAAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            99..>353
                     /locus_tag="ACHLIF_005702"
     CDS             99..>353
                     /locus_tag="ACHLIF_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609439.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVF"
BASE COUNT           77 a           89 c          111 g           76 t
ORIGIN      
        1 aaatcggcaa aaggtgacgc ggcgaaaaaa gcggctaacc gttttgcggt gccaccacca
       61 ccccgcctgg tggtgaataa tgataatgag ggcaacggat gatacctgtg tggagcacgg
      121 cctgcccgga ctgggcagag cgcctgaaaa aggggctgtc gattattccg gctccgattt
      181 atccggacca ggctgcacat gcactggcga tttttaaaca actgcgaatt gtggatgcac
      241 cgggtagccc gacattcggg gagtcctgtg caccgtgggt gtttgacctg gtggcggccc
      301 tgtttggctc ctacgatgcg cagaccggtg ttcgccatat caaggaagtg ttt
//
LOCUS       DAZGOB010000570          350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZGOB010000570 DAZGOB010000000
VERSION     DAZGOB010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.573"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(2..292)
                     /locus_tag="ACHLIF_005703"
     CDS             complement(2..292)
                     /locus_tag="ACHLIF_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO9609440.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
BASE COUNT           70 a          108 c           69 g          103 t
ORIGIN      
        1 gtcatctttt aactccatat accgccaata cccgtttcat cgcggcactc tggcgacact
       61 ccttaaaaat taggttcgtg ctcatctttc cttcccgttc ttccttggta gcaaaccggt
      121 aatacaccgt tcgccagacc ttaccttcga taaccagaag acctgcccgt gccattttag
      181 ccgcggcctg atttatgctg gttactgttg cgcctgttag cgcggcaacg tccggcgcac
      241 agaagctatt atgcgtcccc aggtaatgaa taattgcctc tttgcccgtc atacacttgc
      301 tcctttcagt ccgaacttag ctttgagttc tgcgatcttc gccagagcct
//
LOCUS       DAZGOB010000571          349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZGOB010000571 DAZGOB010000000
VERSION     DAZGOB010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.574"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHLIF_005704"
     CDS             complement(<1..245)
                     /locus_tag="ACHLIF_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9609441.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVS"
     gene            complement(245..>349)
                     /locus_tag="ACHLIF_005705"
     CDS             complement(245..>349)
                     /locus_tag="ACHLIF_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9609442.1"
                     /translation="PYAWRQIKVTCAAWSSRVRMLRVEFSAEFKQVVN"
BASE COUNT           76 a          122 c           71 g           80 t
ORIGIN      
        1 gacaccgtca gcgacggtct ggcactgctg cccttcccgt tcatctcaaa accgctgccg
       61 tcaatcgggt atacctgata ttgccgcccc tgccaggtga ccgcctcccc tttttcattc
      121 agctcattac agaaaaaata ccgctcaccg ccctgcaccg tcaggtcaat ttcccagagc
      181 accacccgcg gtgactgctc tgatttaacc gactcgttca gactttcttc gtgaatatcc
      241 tgcatcagtt caccacctgc ttaaactcgg cactgaattc aacccgcagc atgcgaaccc
      301 gtgatgacca ggcggcacag gtcaccttta tctgccgcca ggcataagg
//
LOCUS       DAZGOB010000572          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZGOB010000572 DAZGOB010000000
VERSION     DAZGOB010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.575"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..178)
                     /locus_tag="ACHLIF_005706"
     CDS             complement(<1..178)
                     /locus_tag="ACHLIF_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HFO9609443.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNESSKI"
BASE COUNT          115 a           52 c           71 g          106 t
ORIGIN      
        1 caatttttga agattcatta ttgaataaat aacaataaaa aaacaggacc tcagattgag
       61 gtgcacactc cagtatccat tcatgaccat cgtgatcatg gaaagatagg gcgttagttt
      121 gttcttcaag agttattccg aacgtatgtg caagatctgt gcgaaattgt tcattcatat
      181 tgtagtcctg tcattctttc aatgagggta tgcttaaacg taattaactc gtgagctctg
      241 gcacaaataa taagacgaaa atataagatg caagtaatag tgttgcatat ttatgatagc
      301 atgtgataaa tgtagtgcaa ctacttgtag atagtgccgg agga
//
LOCUS       DAZGOB010000573          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZGOB010000573 DAZGOB010000000
VERSION     DAZGOB010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.576"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            25..>344
                     /locus_tag="ACHLIF_005707"
     CDS             25..>344
                     /locus_tag="ACHLIF_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO9609444.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKA"
BASE COUNT          124 a           67 c           79 g           74 t
ORIGIN      
        1 gtagcctttt gaagaggatc agaaatggga agaaggcgaa gtcatgagcg ccgggattta
       61 ccccctaatc tttatataag aaacaatgga tattactgct acagggaccc aaggacgggt
      121 aaagagtttg gattaggccg agacaggagg atagcaatca ctgaagcaat acaggccaac
      181 attgagttat tttcaggaca caaacacaag cctctgacag cgagaatcaa cagtgataat
      241 tctgttacgt tacattcatg gcttgatcgc tacgaaaaaa tcctcgccag cagaggaatc
      301 aagcagaaga ctctcataaa ttacatgagc aaaattaaag caat
//
LOCUS       DAZGOB010000574          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZGOB010000574 DAZGOB010000000
VERSION     DAZGOB010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.577"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>335)
                     /locus_tag="ACHLIF_005708"
     CDS             complement(<1..>335)
                     /locus_tag="ACHLIF_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO9609445.1"
                     /translation="EITATLVKPVPDMPSQLAARLDEVWAAIKTGLVRGLSVGFRPHE
                     YTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFSAASGNRKPVVKIASS
                     AGAAAQSTTV"
BASE COUNT           67 a          103 c          101 g           64 t
ORIGIN      
        1 acggttgttg actgtgccgc agcgccggca gaagatgcga ttttcaccac cggtttccgg
       61 ttgccggacg cggcagaaaa cgggcggtcg taagatttaa tggtccggat ggtgcattcc
      121 gcattcgcgg gcacggtgac ggcagacacc tccatcagtt cccagcgcag aaaatgcagt
      181 ccgcctccgt ccagaaaggt gtattcatgg ggacggaagc ccacggacag ccccctgacc
      241 agcccggtct taatggccgc ccagacctca tccagccggg cagccagttg cgacggcata
      301 tccggtacgg gcttcaccag tgttgccgtg atttc
//
LOCUS       DAZGOB010000575          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZGOB010000575 DAZGOB010000000
VERSION     DAZGOB010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.578"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>335
                     /locus_tag="ACHLIF_005709"
     CDS             <1..>335
                     /locus_tag="ACHLIF_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9609446.1"
                     /translation="DPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQ"
BASE COUNT           87 a           77 c          113 g           58 t
ORIGIN      
        1 gacccgacgt cgggactgat ggcgatggcg cgccagttcc gtaacgtgac ggcagagcag
       61 attgcgtatg ttgcacagct gcagcgttcc ggagacgagg ccggggcatt gcaggcggcg
      121 aacgatatcg ccacgaaagg ctttgatgag cagacccgtc gcctgaaaga aaacatggga
      181 acactggaga cctgggcgga taaaacaggg aaggcattca aatcgatgtg ggatgccatt
      241 ctggatatcg gtcgtccgga atcctcagcg gatatgctcg ccagtgcgca gaaggcattt
      301 gatgaggcgg ataaaaaatg gcagtggtac cagag
//
LOCUS       DAZGOB010000576          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZGOB010000576 DAZGOB010000000
VERSION     DAZGOB010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.579"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>335)
                     /locus_tag="ACHLIF_005710"
     CDS             complement(<1..>335)
                     /locus_tag="ACHLIF_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO9609447.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIA"
BASE COUNT           78 a           96 c           91 g           70 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg ccccagtttg
      301 gcgatttcat ccgccagata agcggcaacc tggta
//
LOCUS       DAZGOB010000577          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZGOB010000577 DAZGOB010000000
VERSION     DAZGOB010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.580"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..266
                     /locus_tag="ACHLIF_005711"
     CDS             <1..266
                     /locus_tag="ACHLIF_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO9609448.1"
                     /translation="APWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTDRFG
                     QVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
BASE COUNT           63 a           85 c           92 g           83 t
ORIGIN      
        1 ttgccccctg gttaccccgt gatgaaacaa cggcgctcac cgaggaggca ttcatcgggt
       61 gtatggccac catcaccggt tatcacgcag tggcgggttc cccctgtgaa ggtcgcttca
      121 cagaccgctt tgggcaggtt cattacctgt tgctggagcc ggaggccggg aaagaattca
      181 gacgaggaga caaagtgatt attgtctgcc gtctttccgg ttcccgctgg ctgggcgaac
      241 tgaatccctg gccggatgtt ctttaatccc gacatataca gaggttctca ctatgtttaa
      301 tcttccggga tggttgtagt tgc
//
LOCUS       DAZGOB010000578          321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZGOB010000578 DAZGOB010000000
VERSION     DAZGOB010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.581"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>321)
                     /locus_tag="ACHLIF_005712"
     CDS             complement(<1..>321)
                     /locus_tag="ACHLIF_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO9609449.1"
                     /translation="QVQSDLPWWLVCRGEIHKFRCVPHLTGRRFEHGVTDCYTLFRDA
                     YHLAEIEMPDFVREDDWWRNGQNLYLDNLEATGLYQVPLSAAQPGDVLLCCFGSSVPN
                     HAAIY"
BASE COUNT           86 a          110 c           73 g           52 t
ORIGIN      
        1 gtaaatagcg gcgtgattcg gcaccgatga accaaaacag cacagcagca catcgcccgg
       61 ctgcgccgct gacaacggca cctgatacag ccctgtggcc tccagattat ccagatagag
      121 attctgaccg ttacgccacc agtcatcctc acgcacaaaa tccggcatct caatctccgc
      181 cagatgataa gcatcccgga acagtgtgta acagtccgtc acaccgtgct caaagcgccg
      241 cccggtgaga tgcggcacac agcggaactt atgaatctca ccccggcaga ccagccacca
      301 cggcaaatca ctctgcacct g
//
LOCUS       DAZGOB010000579          318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZGOB010000579 DAZGOB010000000
VERSION     DAZGOB010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.582"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..265)
                     /locus_tag="ACHLIF_005713"
     CDS             complement(<1..265)
                     /locus_tag="ACHLIF_005713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609450.1"
                     /translation="MPLYEWLINKRLRYQYLTLLAFSILALLALYLLYRNTPKVSVNF
                     FEFYHKNLRGYLFSGFISVGSFLLSLHTFVIINLRDKVFATQEY"
BASE COUNT          109 a           54 c           56 g           99 t
ORIGIN      
        1 tatattcctg agttgcaaaa accttatcgc gtaaatttat tatgacaaag gtatgcaggc
       61 tcaataaaaa tgagcccacg gaaataaaac cggagaagag atagcctcgt aagtttttat
      121 gataaaactc aaaaaagtta acacttactt taggtgtgtt tctgtacaat aggtaaagtg
      181 caagcaatgc caggatcgag aatgcaagca gtgtaaggta ctgatacctc aatcttttat
      241 ttattagcca ttcatataaa ggcattttta ttcgtcccgt tcagcatttc ttcttttatc
      301 atatcaaaaa cagggttt
//
LOCUS       DAZGOB010000580          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZGOB010000580 DAZGOB010000000
VERSION     DAZGOB010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.583"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>314
                     /locus_tag="ACHLIF_005714"
     CDS             <1..>314
                     /locus_tag="ACHLIF_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO9609451.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VY"
BASE COUNT           80 a           70 c           88 g           76 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acat
//
LOCUS       DAZGOB010000581          313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZGOB010000581 DAZGOB010000000
VERSION     DAZGOB010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.584"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..263
                     /locus_tag="ACHLIF_005715"
     CDS             <1..263
                     /locus_tag="ACHLIF_005715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFO9609452.1"
                     /translation="RRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEKEFRHCIWLAEK
                     EERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
BASE COUNT           95 a           81 c           71 g           66 t
ORIGIN      
        1 cacgccgctg ttccggcaat tccgtatcgg aggtgctgga taaattcaga aaaaactacg
       61 atcggataat gtcgctaccg caggaaacga aagaggaaaa ggaatttcgc cattgtatat
      121 ggcttgcaga gaaagaagaa cgcgagcgaa tttaccagac atcaatccga ccattccgca
      181 aagccacata tacccacttc cctgaaattg acccgcgcct gcgtaattac cgctcacgct
      241 atggcgctat cagtaatgac tgaggaattt accatgagag gacttgcata caatcccggc
      301 attcttccgg cag
//
LOCUS       DAZGOB010000582          312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZGOB010000582 DAZGOB010000000
VERSION     DAZGOB010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.585"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>312
                     /locus_tag="ACHLIF_005716"
     CDS             <1..>312
                     /locus_tag="ACHLIF_005716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO9609453.1"
                     /translation="LLRWNYAAASDQVRMNILKEYGGIYTDTDILPAYSDEVSQIINK
                     KSDGDMFFEDLKLRRFISEAILSLIKGEKYSIKHDSLDEKTRNQLNAILSEIEKLTID
                     N"
BASE COUNT          123 a           37 c           63 g           89 t
ORIGIN      
        1 tgctattaag atggaactac gccgctgcat cagatcaggt aagaatgaat atattgaaag
       61 agtatggagg gatttatacc gatactgata ttcttcctgc atattcagat gaagtgtcac
      121 agataataaa taaaaaaagt gatggagata tgttttttga agatttaaaa ctaagaaggt
      181 ttatatcaga agctattctg tccctcataa aaggggaaaa gtactcaatt aagcatgata
      241 gcttagatga aaaaacacgt aatcagttaa atgctatatt gagtgaaata gaaaagctga
      301 cgatagataa tt
//
LOCUS       DAZGOB010000583          302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZGOB010000583 DAZGOB010000000
VERSION     DAZGOB010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.586"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>302)
                     /locus_tag="ACHLIF_005717"
     CDS             complement(<1..>302)
                     /locus_tag="ACHLIF_005717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9609454.1"
                     /translation="QDIAKMRLRLMQTDVQGIRREKRQGDTARLCRRPNAQQNRIQFF
                     ELWLNSKLRHGNTVVLKIRNPRGQIKELRILDWNRVEPLVADDGDVFYRITPDRNC"
BASE COUNT           63 a           90 c           78 g           71 t
ORIGIN      
        1 cacagttccg gtccggcgtg atgcggtaga acacgtcgcc gtcatccgcc accagaggtt
       61 caacccggtt ccagtccaga atacgcagtt ctttgatctg cccccggggg ttgcggattt
      121 tcagcaccac cgtattgccg tgacgcaatt tggaattcag ccacagttca aaaaactgga
      181 tgcgattctg ctgcgcatta ggacgacgac agagacgggc agtatctccc tgccgctttt
      241 cccggcgtat tccctgtaca tcagtctgca tcaggcgaag ccgcattttg gcaatatcct
      301 gg
//
LOCUS       DAZGOB010000584          297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZGOB010000584 DAZGOB010000000
VERSION     DAZGOB010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.587"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            5..>297
                     /locus_tag="ACHLIF_005718"
     CDS             5..>297
                     /locus_tag="ACHLIF_005718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609455.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKA"
BASE COUNT           80 a           68 c          106 g           43 t
ORIGIN      
        1 gacaatgggc aaaggtggcg gcaaggcgca cacgccggta gaggcaaagg acaatcttaa
       61 gtccacgcag atgatgagcg tgattgatgc gattggtgag ggaccgatag aaggtccggt
      121 gaagggactg cagagtattc tggtgaacaa aaccccactg acggacacgg acggcaatcc
      181 cgtgatacac ggtgtgaccg cggtctggcg tgccggggag caggagcaga caccaccgga
      241 aggctttgag tcctccggag ctgaaaccgg actgggcgtg gaagtgacga aggcaaa
//
LOCUS       DAZGOB010000585          297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZGOB010000585 DAZGOB010000000
VERSION     DAZGOB010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.588"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>297)
                     /locus_tag="ACHLIF_005719"
     CDS             complement(<1..>297)
                     /locus_tag="ACHLIF_005719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO9609456.1"
                     /translation="LSIIPENFFTLLSGNFKADWRWDVSKETFPKDKFAGYVKSVFSK
                     IDFYKQCGVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELL"
BASE COUNT          102 a           66 c           45 g           84 t
ORIGIN      
        1 tttaataatt cacataataa actccaaccc ttttccctga tgcctatatg ccctgaaacg
       61 agcaatgcat aaagtacagc gcctactctt ccatctgtat cgccaaaata agccgtgttg
      121 gcattttgtg ggttaatcac accacattgt ttataaaagt ctattttaga aaacacagat
      181 ttaacatagc cagcaaactt atccttagga aacgtctctt tagagacatc ccaacgccag
      241 tcagctttaa aattaccgct aagaagagta aagaaatttt caggtatgat agaaagc
//
LOCUS       DAZGOB010000586          296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZGOB010000586 DAZGOB010000000
VERSION     DAZGOB010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.589"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>296)
                     /locus_tag="ACHLIF_005720"
     CDS             complement(<1..>296)
                     /locus_tag="ACHLIF_005720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9609457.1"
                     /translation="RQAAAAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHL
                     ISLADEWSSVNARLKQASQSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAAS"
BASE COUNT           63 a           95 c           85 g           53 t
ORIGIN      
        1 gaggcagccg aacgggcaaa cagggccgca ttatccgaaa atgcggtgcc tgtccgctgg
       61 ctgatatcca tcagcacttt ctgtgatgac gcaaattcat ccgatgactg cgacgcctgt
      121 ttcagacggg cattcacgga gctccattca tccgccagcg aaatcaggtg tccggtggca
      181 aaggcaccgg caaacgcacc ggtcattccg acagccgaag cgcggatttc cgtcaactgg
      241 ctgtgcagct cagccagagc ccggcgctgc tccctggctg ccgcagcagc ctgacg
//
LOCUS       DAZGOB010000587          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZGOB010000587 DAZGOB010000000
VERSION     DAZGOB010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.590"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..257
                     /locus_tag="ACHLIF_005721"
     CDS             <1..257
                     /locus_tag="ACHLIF_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9609458.1"
                     /translation="KFAARLGVSPSYLSQMASGRTAISPTRALMIESATEGQVSRAEL
                     RPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
BASE COUNT           76 a           63 c           80 g           75 t
ORIGIN      
        1 agaaattcgc ggccagactt ggtgtatcgc catcttactt atcgcaaatg gcgtctggac
       61 gaacagctat atctccaacc cgcgccctta tgatcgaatc tgcgacggaa ggccaagtaa
      121 gtagggcgga gctacgacct catgattggg agcttatttg gcctgagtat gcgagcggca
      181 ttcgtttggg gcaaacacat gtagttcatg ctgaaggtga ttgtagtgca tgcttatctg
      241 atggagttga ttcatgaaaa tcaagcatga acacatccgc atggcgatga atgt
//
LOCUS       DAZGOB010000588          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZGOB010000588 DAZGOB010000000
VERSION     DAZGOB010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.591"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>294
                     /locus_tag="ACHLIF_005722"
     CDS             <1..>294
                     /locus_tag="ACHLIF_005722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9609459.1"
                     /translation="CDAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEM
                     YGRQGVELSRSLLSGWVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPG"
BASE COUNT           48 a           90 c           98 g           58 t
ORIGIN      
        1 gtgcgatgcc atcgtgcagg cccccgcgcc ttcacggccc atcgagcggg gtatcgcagg
       61 accggggctg ctggcccgcg tgctgatctc aaagtatgca gagcacaccc cgctgtaccg
      121 ccagtctgaa atgtacggcc gccagggcgt ggagctgagt cgttcactgc tgtcgggctg
      181 ggtggatgca tgctgccggc tactgtcacc gctggaagaa gcgcttcagg actatgtgct
      241 gactgacggt aagctccatg ctgatgacac gcctgtcccg gtgctgttgc cagg
//
LOCUS       DAZGOB010000589          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZGOB010000589 DAZGOB010000000
VERSION     DAZGOB010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.592"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>287
                     /locus_tag="ACHLIF_005723"
     rRNA            <1..>287
                     /locus_tag="ACHLIF_005723"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a           67 c           80 g           53 t
ORIGIN      
        1 cctgcttcca ggaaaagcct ctaagcatca ggtaacatca aatcgtaccc caaaccgaca
       61 caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag gaactaggca
      121 aaatggtgcc gtaacttcgg gagaaggcac gctgatatgt aggtgaagtc cctcgcggat
      181 ggagctgaaa tcagtcgaag ataccagctg gctgcaactg tttattaaaa acacagcact
      241 gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc ccggtgc
//
LOCUS       DAZGOB010000590          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZGOB010000590 DAZGOB010000000
VERSION     DAZGOB010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.593"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          100 a           63 c           45 g           77 t
ORIGIN      
        1 tgtcaaacgg ctgaaggcct ttgctcgtgc tgcattgact atatcaatac gctgcctaag
       61 gatgaaacac cccgacgcct catcaatatt cactctaccc acaccatatg aatgataaat
      121 atttaatact gaaaaaacca ttagaccgta taacaaacac tcaatcaata cttaacagaa
      181 cttttatttt tgacaaacat ataatatttt caacaatatc ctgagccagg tatatttcag
      241 tataaggctc tgccggaagg aatctggaag aatgaatatg gcgcg
//
LOCUS       DAZGOB010000591          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZGOB010000591 DAZGOB010000000
VERSION     DAZGOB010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.594"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>284
                     /locus_tag="ACHLIF_005724"
     CDS             <1..>284
                     /locus_tag="ACHLIF_005724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HFO9609460.1"
                     /translation="ADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNP
                     LALNNVMSEQKKTWAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAA"
BASE COUNT           81 a           67 c          101 g           35 t
ORIGIN      
        1 aggcggataa attcgcacag cagcaacggg caaaacgggc cgccattgat gcgaaaagcc
       61 gggggctgac tgaccggcag gcagaacggg aagccacgga acagcgcctg aaggaacagt
      121 atggcgataa tccgctggcg ctgaataacg tcatgtcaga gcagaaaaag acctgggcgg
      181 ctgaagacca gcttcgcggg agctggatgg caggcctgaa gtccggctgg agtgagtggg
      241 aagagagcgc cacggacagt atgtcgcagg taaaaagtgc agcc
//
LOCUS       DAZGOB010000592          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZGOB010000592 DAZGOB010000000
VERSION     DAZGOB010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.595"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>284
                     /locus_tag="ACHLIF_005725"
     CDS             <1..>284
                     /locus_tag="ACHLIF_005725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609461.1"
                     /translation="HLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASV
                     FWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHID"
BASE COUNT           57 a           84 c           75 g           68 t
ORIGIN      
        1 catctgactt atactcaggc ccgtaatatc acccggttcg ggaccatcga atcggtcact
       61 tatgatattg ccgagggttc ggttgtcagt ctggcggccg gagggatcag ttttgccctg
      121 acggcatcgg tcttctggct cagcaccggc gatcgcgatg ctgccctgca gacagctgct
      181 gtccaggcag gaaaaacctt cacccgcaca ctggctgtct acgtcacaac ccagcaactc
      241 caccggctca gtgttgttca gggtatgctg aagcacattg attt
//
LOCUS       DAZGOB010000593          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZGOB010000593 DAZGOB010000000
VERSION     DAZGOB010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.596"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..274
                     /locus_tag="ACHLIF_005726"
     CDS             <1..274
                     /locus_tag="ACHLIF_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO9609462.1"
                     /translation="HAGIVVHFAVDAGENLVLRQNTTIGQIDGDMPGSRVKIGSNVDI
                     GANCCIIGLSRKIGDNVKIGAMSFINKDIPSNCTYITKKSGVVLYK"
BASE COUNT           97 a           34 c           63 g           90 t
ORIGIN      
        1 tcatgctgga attgttgttc attttgctgt tgatgctggt gaaaatctgg tgttacgaca
       61 gaatactacc attggacaga tagatggtga catgcctggt tcaagagtaa aaattggtag
      121 taacgttgat atcggagcta attgttgcat cattggatta tcacgtaaaa ttggggataa
      181 tgtaaaaata ggtgcaatgt cttttataaa taaagatata ccatcgaact gcacatatat
      241 aactaaaaag agcggtgttg tattgtataa atagagtaca taaa
//
LOCUS       DAZGOB010000594          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZGOB010000594 DAZGOB010000000
VERSION     DAZGOB010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.597"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>281)
                     /locus_tag="ACHLIF_005727"
     rRNA            complement(<1..>281)
                     /locus_tag="ACHLIF_005727"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           57 a           96 c           57 g           71 t
ORIGIN      
        1 agccttgatt ttccggattt gcctggaaaa ccagcctaca cgcttaaacc gggacaaccg
       61 tcgcccggcc aacatagcct tctccgtccc cccttcgcag taacaccaag tacaggaata
      121 ttaacctgtt tcccatcgac tacgcctttc ggcctcgcct taggggtcga ctcaccctgc
      181 cccgattaac gttggacagg aacccttggt cttccggcga gcgggctttt cacccgcttt
      241 atcgttactt atgtcagcat tcgcacttct gatacctcca g
//
LOCUS       DAZGOB010000595          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZGOB010000595 DAZGOB010000000
VERSION     DAZGOB010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.598"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHLIF_005728"
     CDS             <1..198
                     /locus_tag="ACHLIF_005728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO9609463.1"
                     /translation="LWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEET
                     TRPTLKELAAKLSGGVNGYSR"
     gene            182..>281
                     /locus_tag="ACHLIF_005729"
     CDS             182..>281
                     /locus_tag="ACHLIF_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HFO9609464.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRI"
BASE COUNT           70 a           65 c           91 g           55 t
ORIGIN      
        1 ctgtgggata acaaaaagcg gcgtaacgaa gcgctggact gcctggtgta tgcctacgcg
       61 gcattacgtg tgtccgtgca acgctggcag cttgatctgg ctgtactggc aaaatcccgg
      121 gaagaagaga cgacccggcc aaccctgaaa gaactggcag cgaagctgtc cggaggagtg
      181 aatggttaca gtcgctgaac tgcaggcgct gcgtcaggcg cgccttgatt tattaaccgg
      241 taaacgggtg gtgtctgtcc agaaagatgg acgaagaatt g
//
LOCUS       DAZGOB010000596          276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZGOB010000596 DAZGOB010000000
VERSION     DAZGOB010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.599"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>276
                     /gene="gpFI"
                     /locus_tag="ACHLIF_005730"
     CDS             <1..>276
                     /gene="gpFI"
                     /locus_tag="ACHLIF_005730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9609465.1"
                     /translation="ELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDTDDAA
                     GQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDAL"
BASE COUNT           66 a           69 c           89 g           52 t
ORIGIN      
        1 atgaactgat tgcccgtctc cgctcgctgg gtgaacaact gaaccgtgat gtcagcctga
       61 cggggacgaa agaagaactg gcgctccgtg tggcagagct ggaagaggag cttgatgaca
      121 cggatgacgc agccggtcag gacacgtctg tcagcccgga aaatgcgctg accggacatg
      181 aaaatgaggt ggtatcagca cagacggata ccgtgactga tacggctgct ctggtcacgg
      241 ttgtggcact ggtgacgctg cataccgatg cacttc
//
LOCUS       DAZGOB010000597          276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZGOB010000597 DAZGOB010000000
VERSION     DAZGOB010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.600"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>276)
                     /locus_tag="ACHLIF_005731"
     CDS             complement(<1..>276)
                     /locus_tag="ACHLIF_005731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO9609466.1"
                     /translation="QVYPYVVPLLDGRPSVALPWVVFSLISSVSADVMGGQAESSVSV
                     QIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENRCYRAT"
BASE COUNT           60 a           96 c           73 g           47 t
ORIGIN      
        1 gggttgcacg gtaacagcgg ttttccggaa tatagtcctg catttcactg acggatcccg
       61 gggccagcag cattatggct tcacgggcgt cctgacgtat ctgacgcgcc tgcgtcacag
      121 tcccggcata aacgtctatc tgcaccgaca ctgaggactc cgcctgcccg cccatcacgt
      181 ccgccgacac cgatgaaatc aggctgaaaa ccacccacgg aagcgccacc gacggcctgc
      241 catccagcag ggggaccaca tacgggtaca cctgcc
//
LOCUS       DAZGOB010000598          273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZGOB010000598 DAZGOB010000000
VERSION     DAZGOB010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.601"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            19..95
                     /locus_tag="ACHLIF_005732"
     tRNA            19..95
                     /locus_tag="ACHLIF_005732"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Ile,seq:gat)
     gene            138..213
                     /locus_tag="ACHLIF_005733"
     tRNA            138..213
                     /locus_tag="ACHLIF_005733"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Ala,seq:tgc)
BASE COUNT           71 a           62 c           73 g           67 t
ORIGIN      
        1 gagcagtaaa acctctacag gcttgtagct caggtggtta gagcgcaccc ctgataaggg
       61 tgaggtcggt ggttcaagtc cactcaggcc taccaaattt gcacggcaaa tttgaagagg
      121 ttttaactac atgttatggg gctatagctc agctgggaga gcgcctgctt tgcacgcagg
      181 aggtctgcgg ttcgatcccg catagctcca ccatctctgt agtgattaaa taaaaaatac
      241 ttcagagtgt acctgcaaag gttcactgcg aag
//
LOCUS       DAZGOB010000599          268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZGOB010000599 DAZGOB010000000
VERSION     DAZGOB010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.602"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>268)
                     /locus_tag="ACHLIF_005734"
     CDS             complement(<1..>268)
                     /locus_tag="ACHLIF_005734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO9609467.1"
                     /translation="LKALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSL
                     NELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGV"
BASE COUNT          100 a           60 c           35 g           73 t
ORIGIN      
        1 attacaccag agttaaaata gctaccgtta aaatcctcat tacacaaacg ctcagcgctt
       61 ttactttgca tagcatctac atcaattacc actgccccat attcatcttt aaactctaat
      121 gctattaact cgttcaatga acccttacag acgatatcag catcaagata taataatgaa
      181 tccaatcgcg cagaaaaata atcaaatgaa agcaaacgat aataaataga tactggccag
      241 atatttgatt gaggtagcgc cttcaatg
//
LOCUS       DAZGOB010000600          267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZGOB010000600 DAZGOB010000000
VERSION     DAZGOB010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.603"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            122..197
                     /locus_tag="ACHLIF_005735"
     tRNA            122..197
                     /locus_tag="ACHLIF_005735"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Glu,seq:ttc)
BASE COUNT           70 a           65 c           67 g           65 t
ORIGIN      
        1 taaatagcaa ggcgtttacg cgttgggagt gaggctgaag agaataaggc cgttcgcttt
       61 ctattaatga aagctcaccc tacacgaaaa tatcacgcaa cgcgtgataa gcaattttcg
      121 tgtccccttc gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa
      181 tcccctaggg gacgccactt gctggtttgt gagtgaaagt cgccgacccc aatatctcaa
      241 aacagactgt taagtcttgt ttgagat
//
LOCUS       DAZGOB010000601          267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZGOB010000601 DAZGOB010000000
VERSION     DAZGOB010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.604"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..200
                     /locus_tag="ACHLIF_005736"
     CDS             <1..200
                     /locus_tag="ACHLIF_005736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609468.1"
                     /translation="LLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFRHGT
                     LRFNGNVSEKLLTLLIQELKR"
BASE COUNT           65 a           77 c           66 g           59 t
ORIGIN      
        1 tgctattacc ttcttcagag agcccccagc tacttcctgt gactctcgat gcagctgccg
       61 aacagccaga atcgctcgca gaggacccgg aaaccctcag tatcagctgt gaggtaacgt
      121 tccggcacgg gacgctccgc ttcaatggca atgtcagcga aaagctcctg actctgctga
      181 tacaggaact gaagcgatga tcccgttacc ttccgggacc aaaatttggc tggttgccgg
      241 tatcaccgat atgagaaatg gcttcaa
//
LOCUS       DAZGOB010000602          264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZGOB010000602 DAZGOB010000000
VERSION     DAZGOB010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.605"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..159
                     /locus_tag="ACHLIF_005737"
     CDS             <1..159
                     /locus_tag="ACHLIF_005737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9609469.1"
                     /translation="TGYWFLQLLDRVSPSQWAAIGVLGSLLFGLLTYLTNLYFKIRED
                     RRKAARGE"
     gene            164..>264
                     /locus_tag="ACHLIF_005738"
     CDS             164..>264
                     /locus_tag="ACHLIF_005738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9609470.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDE"
BASE COUNT           52 a           62 c           86 g           64 t
ORIGIN      
        1 acgggctact ggttcctgca gttgctggac agggtttccc cgtctcagtg ggcggcaata
       61 ggcgtgctgg ggagtctgct gtttggtctg ctgacgtacc tgacgaacct gtatttcaaa
      121 atcagagagg acaggcgtaa ggcggcacgg ggagagtaag ctgatgagca ggaaactccg
      181 ctatggttta tcggctgccg ttctggcgct gattgccgca ggtgcttctg cgcctgaaat
      241 cctcgaccag tttctggatg aaaa
//
LOCUS       DAZGOB010000603          262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZGOB010000603 DAZGOB010000000
VERSION     DAZGOB010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.606"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..71)
                     /locus_tag="ACHLIF_005739"
     CDS             complement(<1..71)
                     /locus_tag="ACHLIF_005739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609471.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGA"
     gene            complement(68..>262)
                     /locus_tag="ACHLIF_005740"
     CDS             complement(68..>262)
                     /locus_tag="ACHLIF_005740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFO9609472.1"
                     /translation="GAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGL
                     PVPDATGGRLEILCRLGGEK"
BASE COUNT           56 a           95 c           65 g           46 t
ORIGIN      
        1 gcaccactca gaagctgcaa atcgcggctg atgtcctcca gccccgaaaa atccagcagg
       61 gtttcgatca tttttcacct cccagccgac agagaatttc cagacgtccg ccggtcgcat
      121 ccggcacggg cagcccgaca acgttcagga tccggtcacg ccagggacca ctcagcacat
      181 gaagtcgtga cgctgccgtg atttcccgac cggactgacc gcgcacccag atgcggattt
      241 ccgcctgcgc catttccgca cc
//
LOCUS       DAZGOB010000604          262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZGOB010000604 DAZGOB010000000
VERSION     DAZGOB010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.607"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..71)
                     /locus_tag="ACHLIF_005741"
     CDS             complement(<1..71)
                     /locus_tag="ACHLIF_005741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609473.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGA"
     gene            complement(68..>262)
                     /locus_tag="ACHLIF_005742"
     CDS             complement(68..>262)
                     /locus_tag="ACHLIF_005742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFO9609474.1"
                     /translation="GAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGL
                     PVPDATGGRLEILCRLGGEK"
BASE COUNT           54 a           97 c           65 g           46 t
ORIGIN      
        1 gcaccactca gaagctgcaa atcgcggctg atgtcctcca gccccgaaaa atccagcagg
       61 gtttcgatca tttttcccct cccagccgac agagaatttc cagacgcccg ccggtcgcat
      121 ccggcacggg cagcccgaca acgttcagga tccggtcacg ccatggacca ctcagcacat
      181 gaagtcgtga cgctgccgtg atttcccggc cggactgacc gcgcacccag atgcggattt
      241 ccgcctgcgc catttccgca cc
//
LOCUS       DAZGOB010000605          261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZGOB010000605 DAZGOB010000000
VERSION     DAZGOB010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.608"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>261
                     /locus_tag="ACHLIF_005743"
     CDS             <1..>261
                     /locus_tag="ACHLIF_005743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9609475.1"
                     /translation="VLDIGRPDTAQEMLIKAEAAFKKADDIWNLRKDDYFVNDEARAR
                     YWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEASRLKYT"
BASE COUNT           74 a           57 c           80 g           50 t
ORIGIN      
        1 gtgctggata ttggtcgtcc tgataccgcg caggagatgc tgattaaggc agaggctgcg
       61 tttaagaaag cagacgacat ctggaatctg cgcaaggatg attattttgt taacgatgaa
      121 gcgcgggcgc gttactggga tgatcgtgaa aaggcccgtc ttgcgcttga agccgcccga
      181 aagaaggctg agcagcagac tcaacaggac aaaaatgcgc agcagcagag cgataccgaa
      241 gcgtcacggc tgaaatatac c
//
LOCUS       DAZGOB010000606          260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZGOB010000606 DAZGOB010000000
VERSION     DAZGOB010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.609"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>260
                     /locus_tag="ACHLIF_005744"
     CDS             <1..>260
                     /locus_tag="ACHLIF_005744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9609476.1"
                     /translation="NISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRK
                     YGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFNP"
BASE COUNT          108 a           49 c           43 g           60 t
ORIGIN      
        1 taacatctct aaggatgaat gggaaaacct ccatgcatta aaagaaaagc gtttaaatgg
       61 aatgcctaaa acaacaccca aaagtgacca ggttattatg ttgcaaaatc aatacgttcg
      121 tgaaatgcga aaatatggcg tacgaggttt acgttatgat gcggcaaaac actcaaaaca
      181 tgaacaaata gaaagatcaa taaccccacc gcttaaaaat tataatgagc ggttacacaa
      241 tactaactta tttaacccaa
//
LOCUS       DAZGOB010000607          255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZGOB010000607 DAZGOB010000000
VERSION     DAZGOB010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.denovo.610"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..170)
                     /locus_tag="ACHLIF_005745"
     CDS             complement(<1..170)
                     /locus_tag="ACHLIF_005745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9609477.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSP"
BASE COUNT           77 a           56 c           67 g           55 t
ORIGIN      
        1 atcggcgaaa cgtacagcgc catgtttacc agtcccggaa tttgtgagag atagactttc
       61 tccgtggtga agggatagct ctcacggaaa aagagacgca gaaacagcgg atcaaactta
      121 aatttctgct catttgccgc cagcagctgg gcggttgtgt acatcgacat aaaaaaatcc
      181 cgtaaaaaaa gccgcacagg cggcctttag tgatgaaggg taaggttaaa cgatgctgat
      241 tgccgttccg gcaaa
//
LOCUS       DAZGOB010000608         4995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.guided.219, whole genome shotgun sequence.
ACCESSION   DAZGOB010000608 DAZGOB010000000
VERSION     DAZGOB010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.guided.219"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..135)
                     /locus_tag="ACHLIF_005746"
     CDS             complement(<1..135)
                     /locus_tag="ACHLIF_005746"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="poxA; regulates pyruvate oxidase poxB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P lysine(34) lysyltransferase"
                     /protein_id="HFO9609478.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     Q"
     gene            460..2268
                     /gene="frdA"
                     /locus_tag="ACHLIF_005747"
     CDS             460..2268
                     /gene="frdA"
                     /locus_tag="ACHLIF_005747"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO9609479.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2261..2995
                     /gene="frdB"
                     /locus_tag="ACHLIF_005748"
     CDS             2261..2995
                     /gene="frdB"
                     /locus_tag="ACHLIF_005748"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO9609480.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3006..3401
                     /gene="frdC"
                     /locus_tag="ACHLIF_005749"
     CDS             3006..3401
                     /gene="frdC"
                     /locus_tag="ACHLIF_005749"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO9609481.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3412..3771
                     /gene="frdD"
                     /locus_tag="ACHLIF_005750"
     CDS             3412..3771
                     /gene="frdD"
                     /locus_tag="ACHLIF_005750"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO9609482.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            3834..4967
                     /gene="ampC"
                     /locus_tag="ACHLIF_005751"
     CDS             3834..4967
                     /gene="ampC"
                     /locus_tag="ACHLIF_005751"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO9609483.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
BASE COUNT         1237 a         1298 c         1343 g         1117 t
ORIGIN      
        1 ctggctcata caaggcgtct ccacctccag cactccacga tcggcaaaga aacgacggat
       61 ctccgccata atcgccgcgc gttttaataa gttaggaatg gatgcgctcg gctgccagga
      121 tgccgtttcg ctcatagtta aatctccagt ttttgacaag ggcacgaagt ctactcgcaa
      181 cgcgacggcg agacaaattt tacgcaggaa tcaaacagcg gtgggcagtg actaaaaaaa
      241 gcacgatctg atggtttagt aattaaatta atcatcttca gtgataattt agccctcttg
      301 cgcactaaaa aaatcgatct cgtcaaattt cagacttatc catcagacta tactgttgta
      361 cctataaagg agcagtggaa tagcgttcgc agaccgtaac tttcaggcac ttaccctgaa
      421 gtacggggct gtgggataaa aacaatctgg aggaatgtcg tgcaaacctt tcaagccgat
      481 cttgccattg taggcgccgg tggcgcggga ttacgtgctg caattgctgc cgcgcaggca
      541 aatccaaatg caaaaatcgc actaatctca aaagtatacc cgatgcgtag ccataccgtt
      601 gctgcagaag ggggctccgc cgctgtcgcg caggatcatg acagcttcga atatcacttt
      661 cacgatacag tagcgggtgg cgactggttg tgtgagcagg atgtcgtgga ttatttcgtc
      721 caccactgcc caaccgaaat gacccaactg gaactgtggg ggtgcccatg gagccgtcgc
      781 ccggatggta gcgtcaacgt acgtcgcttc ggcggcatga aaatcgagcg cacctggttc
      841 gccgccgata agaccggctt ccatatgctg cacacgctgt tccagacctc tctgcaattc
      901 ccgcagatcc agcgttttga cgaacatttc gtgctggata ttctggttga tgatggtcat
      961 gttcgcggcg tggtagcaat gaacatgatg gaaggcacgc tggtgcagat ccgtgctaac
     1021 gcggtcgtta tggctactgg cggtgcgggt cgcgtttatc gttacaacac caacggcggc
     1081 atcgttaccg gtgacggtat gggtatggcg ctaagccacg gcgttccgct gcgtgacatg
     1141 gaattcgttc agtatcaccc aaccggtctg ccaggttccg gtatcctgat gaccgaaggc
     1201 tgccgcggtg aaggcggtat tctggtcaac aaaaatggct accgttatct gcaagattac
     1261 ggcatgggcc cggaaactcc gctgggcgag ccgaaaaaca aatatatgga actgggtcca
     1321 cgcgacaaag tttctcaggc cttctggcac gaatggcgta aaggcaacac catctccacg
     1381 ccgcgtggcg atgtggttta tctcgacctg cgtcacctcg gcgagaaaaa actgcatgaa
     1441 cgtctgccgt tcatctgcga actggcgaaa gcgtacgttg gcgtcgatcc ggttaaagaa
     1501 ccgattccgg tacgtccgac cgcacactac accatgggcg gtatcgaaac cgatcagaac
     1561 tgtgaaaccc gcattaaagg tctgttcgcc gtgggtgaat gttcctctgt tggtctgcac
     1621 ggtgcaaacc gtctgggttc taactccctg gcggaactgg tggtcttcgg ccgtctggcc
     1681 ggtgaacaag cgacagagcg tgcagcaact gccggtaatg gcaacgaagc ggcaattgaa
     1741 gcgcaggcag ctggcgttga acaacgtctg aaagatctgg ttaaccagga tggcggcgaa
     1801 aactgggcga agatccgcga cgaaatgggc ctggcaatgg aagaaggttg cggtatctac
     1861 cgtacgccgg aactgatgca gaaaaccatc gacaagctgg cagagctgca ggaacgcttc
     1921 aagcgcgtgc gcatcaccga cacctccagc gtgttcaaca ccgacctgct ctacaccatt
     1981 gaactgggcc acggtctgaa cgttgctgaa tgtatggcgc actccgcaat ggcacgtaaa
     2041 gagtcccgcg gcgcacacca gcgtctggac gaaggttgca ccgagcgtga cgacgtcaac
     2101 ttcctcaaac acaccctcgc cttccgcgat gctgatggca cgactcgcct ggagtacagc
     2161 gacgtgaaga ttactacgct gccgccagct aaacgcgttt acggtggcga agcggatgca
     2221 gccgataagg cggaagcagc caataagaag gagaaggcga atggctgaga tgaaaaacct
     2281 gaaaattgag gtggtgcgct ataacccgga agtcgatacc gcaccgcata gcgcattcta
     2341 tgaagtgcct tatgacgcaa ctacctcatt actggatgcg ctgggctaca tcaaagacaa
     2401 cctggcaccg gacctgagtt accgctggtc ctgccgtatg gcgatttgtg gttcctgcgg
     2461 catgatggtt aacaacgtgc caaaactggc atgtaaaacc ttcctgcgtg attacaccga
     2521 cggtatgaag gttgaagcgt tagctaactt cccgattgaa cgcgatctgg tggtcgatat
     2581 gacccacttc atcgaaagtc tggaagcgat caaaccgtac atcatcggca actcccgcac
     2641 cgcggatcag ggtactaaca tccagacccc ggcgcagatg gcaaagtatc accagttctc
     2701 cggttgcatc aactgtggtc tgtgctacgc cgcgtgcccg cagtttggcc tgaacccaga
     2761 gttcatcggt ccggctgcca ttacgctggc gcatcgttat aacgaagata gccgcgacca
     2821 cggtaagaag gagcgtatgg cgcagttgaa cagccagaac ggcgtatgga gctgtacttt
     2881 cgtgggctat tgctccgaag tctgcccgaa acacgtcgat ccggctgcgg ccattcagca
     2941 gggcaaagta gaaagttcga aagactttct tatcgcgacc ctgaaaccac gctaaggagt
     3001 gcaacatgac gactaaacgt aaaccgtatg tacggccaat gacgtccacc tggtggaaaa
     3061 aattgccgtt ttatcgcttt tacatgctgc gcgaaggcac ggcggttccg gctgtgtggt
     3121 tcagcattga actgattttc gggctgtttg ccctgaaaaa tggcccggaa gcctgggcgg
     3181 gattcgtcga ctttttacaa aacccggtta tcgtgatcat taacctgatc actctggcgg
     3241 cagctctgct gcacaccaaa acctggtttg aactggcacc gaaagcggcc aatatcattg
     3301 taaaagacga aaaaatggga ccagagccaa ttatcaaaag tctctgggcg gtaactgtgg
     3361 ttgccaccat cgtaatcctg tttgttgccc tgtactggta aggagcctga gatgattaat
     3421 ccaaatccaa agcgttctga cgaaccggta ttctggggcc tcttcggggc cggtggtatg
     3481 tggagcgcaa ttattgcgcc ggtgatgatc ctgctggtgg gtattctgct gccactgggg
     3541 ctgtttccgg gtgatgcgct gagctacgag cgcgttctgg cgttcgcgca gagcttcatt
     3601 ggtcgcgtat tcctgttcct gatgatcgtt ctgccgctgt ggtgtggttt acaccgtatg
     3661 caccacgcga tgcacgatct gaaaattcac gtgcctgcgg gcaaatgggt tttctacggt
     3721 ctggctgcta tcctgacagt tgtcacgctg attggtatcg ttacaatcta acgtatcgcc
     3781 aatgtaaatc cggcccgcct atggcgggcc gttttgtatg gaaaccagac cttatgttca
     3841 aaacgacgct ctgcgcctta ttaattaccg cctcttgctc cacatttgct gcccctcaac
     3901 aaatcaacga tattgtgcat cgcacaatta ccccgcttat agagcaacaa aagatccccg
     3961 gtatggcggt ggcggtaatt tatcagggta aaccttatta ctttacctgg ggctatgcgg
     4021 acatcgccaa aaagcagccc gtcacacagc aaacgttgtt tgagttaggt tcggtcagca
     4081 aaacatttac gggcgtgctt ggtggcgacg ctattgctcg aggggaaatc aagttaagcg
     4141 atcccacaac aaaatactgg cctgaactta ccgctaaaca gtggaatggg atcacactat
     4201 tacatctcgc aacctacact gctggcggcc tgccattgca ggtgccggat gaggtgaaat
     4261 cctcaagcga cttgctgcgc ttctatcaaa actggcagcc tgcatgggcg ccaggaacac
     4321 aacgtctgta tgccaactcc agtatcggtt tgttcggcgc actggctgtg aagccgtctg
     4381 gtttgagttt tgagcaggcg atgcaaacac gtgtcttcca gccactcaaa ctcaaccata
     4441 cgtggattaa tgtaccgccc gcagaagaaa agaattacgc ctggggatat cgcgaaggca
     4501 aggcagttca tgtttcgcca ggggcgttag atgctgaaac ttatggtgtg aagtcgacca
     4561 ttgaagatat ggcctgctgg gtacgaagca atatgaatcc ccgtgatatc aacgacaaaa
     4621 cacttcagca agggatacaa ctggcacaat ctcgctactg gcaaaccggc gatatgtatc
     4681 agggcctggg ctgggaaatg ctggactggc cggtaaatcc tgacagcatc attaacggca
     4741 gtggcaataa aattgcactg gcagcacacc ccgtaaaagc gattacgccc ccaactcctg
     4801 cagtacgcgc atcatgggta cataaaacag gggcaaccgg cggatttggt agctatgtcg
     4861 cgtttattcc agaaaaagag ctgggtatag tgatgctggc taacaaaaac tatcccaatc
     4921 cagcgagagt cgccgccgcc tggcagattc tcaacgctct acagtaaaat ttcatcgggt
     4981 ccgaattttc ggacc
//
LOCUS       DAZGOB010000609         3542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.guided.253, whole genome shotgun sequence.
ACCESSION   DAZGOB010000609 DAZGOB010000000
VERSION     DAZGOB010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.guided.253"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..341
                     /gene="prfH"
                     /locus_tag="ACHLIF_005752"
     CDS             <1..341
                     /gene="prfH"
                     /locus_tag="ACHLIF_005752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO9609484.1"
                     /translation="LGIGRFTADEQEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHL
                     ASGISVKVQSERSQHANKRLARLLIAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRR
                     TFTGMAFIEG"
     gene            complement(398..1855)
                     /gene="pepD"
                     /locus_tag="ACHLIF_005753"
     CDS             complement(398..1855)
                     /gene="pepD"
                     /locus_tag="ACHLIF_005753"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO9609485.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2116..2574
                     /gene="gpt"
                     /locus_tag="ACHLIF_005754"
     CDS             2116..2574
                     /gene="gpt"
                     /locus_tag="ACHLIF_005754"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO9609486.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2666..>3542
                     /gene="frsA"
                     /locus_tag="ACHLIF_005755"
     CDS             2666..>3542
                     /gene="frsA"
                     /locus_tag="ACHLIF_005755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO9609487.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKA"
BASE COUNT          833 a          910 c          928 g          871 t
ORIGIN      
        1 ttctgggcat tgggcgtttt accgctgatg agcaggaaca atcggatgca atccgttatg
       61 agacgttgcg ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac
      121 gcgccacgca tttggcatcc ggtattagcg tgaaggttca gtcagagcgt agtcagcatg
      181 ctaacaagcg gctggcacga ttgctgattg cctggaagct ggagcagcag caacaggaaa
      241 atagcgcggt gctgaaatcg ctgcggcgaa tgttccatca ccagattgaa cgtggcaacc
      301 cgagacggac atttacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg
      361 aaggcgatta aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca
      421 gcagttcagt cagcagcgtc cagtaatgac ctacgctttc gatgtgaact tgctcatccg
      481 gagagtgtgg accggtgata gttggcccga tagaaaccat gtccatttcc ggatacggtt
      541 ttttgaacag accacattcc aggcccgcgt ggataatctg gatgttcggt gttttgttga
      601 acagacgctg ataggtttca cgtaccagat gcatcaccgg agaattagcg tccggctgcc
      661 agccaggata tgcgcctttc gcttcggttt tcgcgccagc tagtttaccc agcgaatcca
      721 gcatgctcac cacgtagtct ttaccgctgt cgatcagtga acggatcagg cagtgaattt
      781 ctacgttatt gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt
      841 ttgccacgtc ggaattgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg
      901 tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc tacagagtcc agcaacaagg
      961 ccagattttt ctctttctct gccagctcgt ttttcaggat ctcctgatag gtattcacca
     1021 gagatttcag gacgtcgact ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg
     1081 ggatggcgtt acgcagtgtg ccgccgttga aatcgataag gcgcagatcc agttcttccg
     1141 catgacccgc caggaagcgc accagtagtt tgttggcatt acccaggcca acgtggattt
     1201 caccgccgga gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag
     1261 ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac
     1321 ccatgtagat ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc
     1381 agttgctctg taagccgaac gcaccgtcca taccggcttc ttcggtcatg gtcagcagca
     1441 cttccagcgg gccgtgaacc acgttttcgt cagccagaac cgccagcgca gacgccatac
     1501 caatgccgtt atctgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat
     1561 aaggctggat aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca
     1621 tatcgaggtg ggcctgtaag acgaccggtt tacgattttc catacctgcg gtagcaggtt
     1681 tacgaatcag gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc
     1741 aaccaacaat gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga
     1801 ttttggcaaa aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa
     1861 gtctccttgt cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt
     1921 gtcaacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
     1981 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
     2041 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     2101 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
     2161 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
     2221 agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
     2281 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
     2341 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
     2401 actgctgttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
     2461 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc
     2521 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
     2581 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcaggttac aatagtttcc
     2641 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
     2701 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
     2761 accgcctgtg cagtcggctc ttgatggtaa aaccatccct cactggtatc gcatgattaa
     2821 ccgtctgatg tggatctggc gcggtattga cccacgtgaa atcctcgacg tccaggcacg
     2881 tattgtgatg agcgatgctg aacgtaccga cgatgattta tacgatactg tgattggcta
     2941 ccgtggtggc aactggattt atgagtgggc cacccaggcg atggtgtggc aacaaaaagc
     3001 ctgtgcggaa gaagatccgc aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta
     3061 caacattgcc gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt
     3121 atcaaaccgc gcctatgaag aggccgccca gcgtctgccg ggcacgatgc ggcagatgga
     3181 gtttaccgta cccggcggtg cgcccatcac cggctttttg catatgccga aaggcgatgg
     3241 cccgttcccg acagtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag
     3301 cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg ctgactattg atatgccatc
     3361 ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt
     3421 cttaaaggcg ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg cctttggttt
     3481 ccgtttcggc gctaacgttg ccgtgcgtct ggcatatctt gaatcgccgc gtctgaaagc
     3541 gg
//
LOCUS       DAZGOB010000610         1545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48220
            SAMN43283552-rid23084563.guided.341, whole genome shotgun sequence.
ACCESSION   DAZGOB010000610 DAZGOB010000000
VERSION     DAZGOB010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283552
            Sequence Read Archive: SRR30325530
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351792.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:51:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 96
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 105 of 253
            Pseudo Genes (incomplete)         :: 144 of 253
            Pseudo Genes (internal stop)      :: 60 of 253
            Pseudo Genes (multiple problems)  :: 48 of 253
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283552-rid23084563.guided.341"
                     /serotype="O 26:H11"
                     /isolate="2211S48220"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            120..1079
                     /locus_tag="ACHLIF_005756"
     CDS             120..1079
                     /locus_tag="ACHLIF_005756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO9609488.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1091..1360
                     /locus_tag="ACHLIF_005757"
     CDS             1091..1360
                     /locus_tag="ACHLIF_005757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO9609489.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          415 a          309 c          383 g          438 t
ORIGIN      
        1 cactggttcg aatccagtac aacgcgccat atttatttac caggctcgct tttgcgggcc
       61 ttttttatat ctgcgccggg tctggtgctg attacttcag ccaaaaggaa cacctgtata
      121 tgaagtgtat attatttaaa tgggtactgt gcctgttact gggtttttct tcggtatcct
      181 attcccggga atttacgata gacttttcga ctcaacaaag ttatgtctct tcgttaaata
      241 gtatacggac agagatatcg acccctcttg aacatatatc tcaggggacc acatcggtgt
      301 ctgttattaa ccacaccccg ccgggcagtt attttgctgt ggatatacga gggcttgatg
      361 tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat ttatatgtgg
      421 ccgggttcgt taatacggca acaaatactt tctaccgttt ttcagatttt acacatatat
      481 cagtgcccga tgtgacaacg gtttccatga caacggacag cagttatacc actctgcaac
      541 gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg gtttcatcat
      601 atctggcgtt aatggagttc agtggtaata caatgaccag agatgcatcc agagcagttc
      661 tgcgttttgt cactgtcacg gcagaagcct tacgcttcag gcagatacag agagaatttc
      721 gtcaggcact gtctgaaact gctcctgtgt atacgatgac gccgggagac gtggacctca
      781 ctctgaactg ggggcgaatc agcaatgtgc ttccggagta tcggggagag gatggtgtca
      841 gagtggggag aatatccttt aataatatat cagcgatact gggtactgtg gccgttatac
      901 tgaattgcca tcatcagggg gcgcgttctg ttcgcgccgt gaatgaagag agtcaaccag
      961 aatgtcagat aactggcgac aggcccgtta taaaaataaa caatacatta tgggaaagta
     1021 atacagctgc agcgtttctg aacagaaagt cacagttttt atatacaacg ggtaaataaa
     1081 ggagttaagt atgaagaaga tgtttatggc ggttttattt gcattagttt ctgttaatgc
     1141 aatggcggcg gattgtgcta aaggtaaaat tgagttttcc aagtataatg aggatgacac
     1201 atttacagtg aaggttgacg ggaaagaata ctggaccagt cgctggaatc tgcaaccgtt
     1261 actgcaaagt gctcagttga caggaatgac tgtcacaatc aaatccagta cctgtgaatc
     1321 aggctccgga tttgctgaag tgcagtttaa taatgactga ggcataacct gattcgtggt
     1381 atgtgggtaa caagtgtaat ctgtgtcaca attcagtcag ttgacagttg cctgtcagac
     1441 tgagcatttg ttaaaaaaat ttcgcatggt gaatccccct gtgcggaggg gcgactggtg
     1501 tataaggtgt catctctgac gacaacctgg atcgagatac gcggg
//
